LOCUS       DATISU010000001        21073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_745305, whole genome shotgun sequence.
ACCESSION   DATISU010000001 DATISU010000000
VERSION     DATISU010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 21073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21073
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745305"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(310..1314)
                     /locus_tag="VLR27_00005"
     CDS             complement(310..1314)
                     /locus_tag="VLR27_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01850.1"
                     /translation="MTSADDHFASAIGLRTADPEEPGIVRRRQGRGFSFRWPDGSVVK
                     GANRERCEALVLPPAWTDVWVCRRADGHLQAMGTDDAGRRQYRYHDRWTEARAAANFD
                     RLVEVGESLAGVRRAVADDLEANDRCTRALATMVRLLDTSLERIGNPDSVELHGSRGI
                     STLAPSNIDVSRQSVRLSFTGKGGVEHDVTIVDRDLAAAIADLEADAEEWLFEVDGTV
                     IDAVDANAYLDEHSAGCLDCKDLRTWGGSAAALTARAEGAEKEPEIADAAAAVLHNTR
                     TVARNSYVHPAVFDAEDDQVAEVWGASRRSKWYGRGERALLNLLEGSPPLLEGYTTAR
                     "
     gene            complement(1374..2765)
                     /gene="thrC"
                     /locus_tag="VLR27_00010"
     CDS             complement(1374..2765)
                     /gene="thrC"
                     /locus_tag="VLR27_00010"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HSP01851.1"
                     /translation="MTTTTSTLRYVSTRGDAPQLDFADTMLTGLAPDGGLYVPVQFPR
                     LPDLDGAESYAEVAGAVMWPFLEGTIERATFDAIVADAYATFDHPDVCPLVRLDERRW
                     LLELFHGPTMAFKDVALQLVGRLFDHYLQERGRRATILVATSGDTGSAAMAATVGRAA
                     LDCVVLFPDGGRVSDVQRRQMTTIDAPNVHAVAATGSFDDCQDIVKAAFADRLFRDEV
                     QLSAMNSINWGRVMAQVVYYVTAARAVRAETGQAPVFSVPTGNFGNVLAGWVAQEMGL
                     DVAGFVVASNANDVLTRWLETGAITAEPVVPTISPSMDIQVSSNLERLLFDLLGRDGR
                     ATAELLSEYRATGHVEAPRPHGWWGARLDDDETRAVIAEHHERSGVLLDPHSAVGVGA
                     ADRVADLPDAPVVCLATAHPAKFPDAVESATGVRPPLPPHYADLYEREERVEEVSAEL
                     PAVQALVRRLIQH"
     gene            complement(2762..4048)
                     /locus_tag="VLR27_00015"
     CDS             complement(2762..4048)
                     /locus_tag="VLR27_00015"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HSP01852.1"
                     /translation="MAPTTIRVGLLGCGVVGGSLVGLLASRGDDIEARTGLRFELGPV
                     AVRSLDKARSAEIDPSHLTTDALSVVEDPDVDVVIELMGGLEPAGTLVAKALAAGKPV
                     ITANKALLAGSGADLFAIADAAGVDLLFEAAVAGGIPFIRPLRESLAGEHISRVMGIV
                     NGTTNYILTKMTEDGAEYADALAEAQALGYAEADPTADVEGHDAAAKAAIIGSIAYGS
                     RIVTDDVYVEGITNIGASDIEQATKLGYVVKLLAIVESFGVDGIAARVHPTLVPETHP
                     LASVRDSFNAIFVEGAAVGDLMFYGRGAGGGPTASAVLGDLIDAAGNLRKRTHASIYT
                     SDPAHIVPIEQLRSEYYLNLDLHDRPGALAAVAGIFGQRDVSIRSMEQIDVGADARVL
                     FITHTAREADLRATLDDLREHPDVGQVNTVLRVLGA"
     gene            complement(4109..5383)
                     /gene="lysA"
                     /locus_tag="VLR27_00020"
     CDS             complement(4109..5383)
                     /gene="lysA"
                     /locus_tag="VLR27_00020"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HSP01853.1"
                     /translation="MAGPLPTHLLPEHSAIDADGRLSIGGCDLLEVAAEFGTPLFVYD
                     EQHLRNRCREAVAAFPDGVAYATKAFLCVAMAKLVHEEGMDLDVATGGEMHVALTAGV
                     PADKLVLHGNNKSLAELAYARTVGVGRIVVDSFDELERLTRLHQDDGYAPKVLLRITP
                     GIEAHTHEFVMTGQEDSKFGFGLRSGAAGRAIELASASPAVDLVGIHMHIGSQVFVAD
                     SFHDAVEAMVPFVTTHDLPEFSIGGGLGVPYVEGETAPTMADWGGAIQAAAREAGITA
                     RITAEPGRSIAAAAAVTLYTVGTIKDVPGVRTFLSVDGGMSDNPRPVLYGSGYETFLP
                     RATDAERTKPVRVVGKHCESGDLLVREGWVPDDVAVDDVLATPVTGAYGHSMGSNYNK
                     ILRPAVVFCRDGDARSVVRRESFDDLVRNDVF"
     gene            complement(5383..7005)
                     /gene="argS"
                     /locus_tag="VLR27_00025"
     CDS             complement(5383..7005)
                     /gene="argS"
                     /locus_tag="VLR27_00025"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HSP01854.1"
                     /translation="MIRDTLAAAVESAAAGIGLDPAAVTWTMERPARREHGDWSCNIA
                     MASAKAAGRPPREIAGDLVAALEQADIAHVDAVEIAGPGFVNFRLAPSWLHDVLVEVV
                     EGGTDGYARPDVGGGTKVNIEFVSANPNGPLHAGHGRGAAYGDSVARIMERTGFDVTR
                     EFYLNDRGVQMARYAESLLARKRGEPVPEDGYGGEYVADWAAEMPDDVADAGIDAVRA
                     WGYDRALADQREVLAAFHVHFDVWSSEQALVDSGAMDAALADLRERGHAYDADGATWL
                     RSTDFGDDKDRPLLKSDGDPTYLLPDIAYHRDKFARGFDLLIDVWGADHHGYVPRMRA
                     GIEALGHDPDEFEVPIVQIVRLERDGKEVRLSKRAGDIIELRDLIDEVGADVARLTYL
                     LQSVDSQQAIDLDLVTSRSNDNPVFYVQYAHARIHSIMGKAAAAGVVRAPLADADLSL
                     LTHEREIELLRTLSELPEVLVRAARERAPHTITNWVRELADRFHGFYHDCYVIGDVPA
                     ELTQARLWLVDAARVGFAIGLDLLGVSAPESM"
     gene            complement(7043..7420)
                     /locus_tag="VLR27_00030"
     CDS             complement(7043..7420)
                     /locus_tag="VLR27_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSP01855.1"
                     /translation="MPAPTVLVVEDDPTILQLLEVNFEMEGYIVLRAEDGEQGLAVAR
                     ESRPDVIVSDVMMPKMSGLELVQALKTSAATKAIPVILLSAKAQGADVRMGLEAGADD
                     YVTKPFEPLDLIDRVNAVLLRNG"
     gene            7505..7579
                     /locus_tag="VLR27_00035"
     tRNA            7505..7579
                     /locus_tag="VLR27_00035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7538..7540,aa:Arg,seq:ccg)
     gene            7739..8251
                     /locus_tag="VLR27_00040"
     CDS             7739..8251
                     /locus_tag="VLR27_00040"
                     /inference="COORDINATES: protein motif:HMM:NF018765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HSP01856.1"
                     /translation="MTTTPGERFRQHAEEYSEDADDERPLGAYTVLLVAYISVVTALV
                     VFGRKRLPGTLAPSDLALGAFATFIGTRTLTKHPIASPIRMPFTKFAGVSGPSELEED
                     VRVSGWRHAIGELLTCPFCLSQWMATTLVTGVVVAPRLTRTVMSVLAIVGASDFLQLA
                     YVRAQNLATD"
     gene            complement(8268..9629)
                     /locus_tag="VLR27_00045"
     CDS             complement(8268..9629)
                     /locus_tag="VLR27_00045"
                     /inference="COORDINATES: protein motif:HMM:NF027518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HSP01857.1"
                     /translation="MPARRRWVAVAIPLVVLAVAALLRLVDLGHIERIHFDESFYAND
                     ANALVERGVEVERPAHPPVGKWVLAAGIAVLGFDPVGWRIGSAVAGTAMVLVTYGAGL
                     LLLRDRWLAAAAALLVAVDGLAITTSRIAMLDAVLALFVSLALLLLLRYHRVRARAPV
                     TRRFTPAGPELWLAGVALGLAVGTKWSAAPMLAVAVALVGWTEVRRRRPGRGTLRAVL
                     EGGVATVLPLVVVPFVVYVASYGGFFLNYTESKVYEQRCPELDCDAPVTERVAGLVFH
                     HRDLVRMHRALEHTHPYRSAAPTWPILERPVLSYLERCPTTDGAPCVVPEGTTARVVM
                     VGNPALWWTMLAALPLVLWQVVRRRERALVGFLALGASLYVPWLGLGRGYIFYLAPLV
                     PFAALALVAGTRSLPGRAARIAPWVVVAAAVAMLAFLFPIWYGLPLDADATEARLLLD
                     SWR"
     gene            complement(9632..11110)
                     /locus_tag="VLR27_00050"
     CDS             complement(9632..11110)
                     /locus_tag="VLR27_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSP01858.1"
                     /translation="MHQLGNFVRRHRASALALGACVVLGALAGALSFALQPDTVGSLG
                     PGSVTIDVGVRASETNVDLPPLGHLVADSHRGPVGFDVRVDRIDLAQAGALAGELARG
                     SDPAQRLRADIDDDLSPLLRSLVLRSLGVALVAGVVVGLLLPQRRVRYVAATTAGSMG
                     FVVVAGAMAFASFSPTAFDQPRFEGSLAAAPDIVNTVQRHIDEVGVVESRLEALSARL
                     VGLYRSVEGTGPSLTDTTLLHVSDLHSNPVGIELVEQTAERFDVDAIIDTGDITSFGS
                     SVEQLVVQRIARFGVPYYLVPGNHDHSSIRRALVDAGVEVLDPGVAEVGDVRILGMGD
                     PAFTADNQLARDDYDELLARASESLARRVQLTRPDVVAVHNSDQLDAAIGTFDVGLAG
                     HTHRFAVEYREGSVVAHSGSAGATGVGALTESADLPYEMQLLQFDGSRLVAIDRLSFE
                     GTEGAFRLERVLIDPTEVDGYPDPEAITFERGPFGPFLRFAN"
     gene            complement(11155..11571)
                     /locus_tag="VLR27_00055"
     CDS             complement(11155..11571)
                     /locus_tag="VLR27_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP01859.1"
                     /translation="MDSTDDIGGGREVRVVIADDNALLVDTLVVALTRHPQLRVVATA
                     GNGPATLAAVAEHRPDVVLMDLRLGGTWGLDLVPEMRATDPAPAVVVFSAALDDAARR
                     AVEVGEVDGHVRKGAPLDELLGALLAAGSPADPPSA"
     gene            11637..12707
                     /locus_tag="VLR27_00060"
     CDS             11637..12707
                     /locus_tag="VLR27_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HSP01860.1"
                     /translation="MLDIPDSAEQARLGQLRVHLETVQRIAGLGSWEADVRTEELTWS
                     ANVYRILGLPETVEPSYDRFLGAVHPDDRHRIARAHADAIEGRNAYSLEHRIVRPDGT
                     IRHVHEEATAEREDGEVIRLLGTMQDITDQMALTRSLIDTESRRRELLHRLVRASEHE
                     RAQLAGDLHDGPIQMLTVAAMRLEHLGMVETDTPDWLPEAVQTMRETVVQLRDVLVEL
                     HPRAGAGAGLEATLTQLALTVVPDLDVRIEVRGDADDAESRAVFGIVQEALWDVREGD
                     RSRHLDIRVDVGEDALEVVLTGARRADGRGPELLQRAGLLGVRERTEGVGGRCTVDST
                     DGIPAVRCTLPRTAPEIAAPRA"
     gene            12700..13737
                     /locus_tag="VLR27_00065"
     CDS             12700..13737
                     /locus_tag="VLR27_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HSP01861.1"
                     /translation="MPEESTELNRVLVHLQSAHRLAGIGSWEAEVAGPPRLLWSAEVH
                     EIAGLPLDQQPTFEDFVAMVHPDDRALFLDARAAALSGERPYDIDLRVILPDGGQRQI
                     HIVAEVVRDEGGNAVRLVGAVQDRTEELGALRRLRLTEVARRDLLQRLLDSADFERNR
                     LGRHLESGPIDRLVEIEQRLAREIPDGASPRWTDALISVRKAIDSLHQTLTDIRAEPS
                     TGDLAQILEDLAAETAPGVHMSIEVDVAASLRPPVQATLLRVVQEALHNVRKHAGAAH
                     ASVRCVVDGSCVDVTVADDGRGFDVDTVERLPGHLGLVAMRDRLVSLGGHLDVRSRPG
                     RTVVEARIPLA"
     gene            complement(13755..14414)
                     /locus_tag="VLR27_00070"
     CDS             complement(13755..14414)
                     /locus_tag="VLR27_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP01862.1"
                     /translation="MTERQSAACRVVIVDDHAMVRESIALALGRSPELDVVGSASSVR
                     EGRAMVQELHPDVAVLDYSLPDGTGIELARSIHEDEPPVRTILLTASEGVQAAAEAVA
                     AGCSGFVRKSADLDDLAAGVLRVHSGEAIFDAQTLSAAIGWLNRPTGPPVGLTERELD
                     VLRCLAEGRTTVDISELLYLSHHTVRNHVRNILGKLGARSQLEAVVIGASKGLVSVGR
                     D"
     gene            14509..15639
                     /locus_tag="VLR27_00075"
     CDS             14509..15639
                     /locus_tag="VLR27_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP01863.1"
                     /translation="MDDGRDRHARHVRGLVAAGDVLARPGLSAGSRLRRLCDIAHAAL
                     GAHRTAVVVTDDRGAFHIDHLVGAPLTEAEEMFGLARLGDHETVERLTRREAIVVDEG
                     AQLPGRGALVVCPMALDGDLWGCLCVEGAPDGRQHVDDLTVDTLRGLANMAAHVVLAE
                     QLRIDRAEILSRELLTEQRNRRRLANRLHDGPQQILVSLRMRLQMLQRDVSDELEREV
                     GLLADHVESARDELRGLILDLDTMTGHAMLSNALRSLLETNSAMAGWQTSFHAEEGVD
                     DVNEEAQITIERVVQEALANARQHAEASSVQLRITTSGDMVTVAVIDDGVGIPSHVST
                     ASTGSGGIRAMHERIWLVGGTLHIESDAGTGTRVVITAPRHA"
     gene            15721..16407
                     /locus_tag="VLR27_00080"
     CDS             15721..16407
                     /locus_tag="VLR27_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP01864.1"
                     /translation="MQNPRTAPPGDQVLARRVTSVALIEDQQVLAGAIQRSLSVMGDL
                     RVVGSATSLGEGVALVRQTQPDVLVSDYRLGDGDVPSRLAEILAGSPETRVLVFTGWP
                     DENSFLEAMAAGASGFLDKAGPFEEFIDAIRRVAAGEVVVAPRFLPALTRRATNADST
                     ALSRRELEVLQLLAEGLSTAEVADRLILSVNTVRNHITHVLTKLGVRSRLEAVNVGVR
                     RGLIRFDPPA"
     gene            complement(16417..17235)
                     /locus_tag="VLR27_00085"
     CDS             complement(16417..17235)
                     /locus_tag="VLR27_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01865.1"
                     /translation="MSISDGSHAPVRGTLAVVAGPDVDAGVLGILAAAGHEVRCLPGL
                     PDAIALARTEPVAVLAVAAEALDEAHAAAGRWTSALPGVGELFALHRADDVPSMWRSY
                     RRGAVPWVLGSTSVPGLAPPEMSGPDLALDALHEELVGRHTEACRLLAAIRDQLVIAE
                     AIEVLVAAGASALERLRGRSRYPVPADLLVQHLPPHVGTLEGVDFALWWGMVESVAFR
                     ASGLRLPAFDLDLAERPHAVAFGAFHAASTIVRFTARRQWRQPVDLLGDLAAVE"
     gene            complement(17232..17735)
                     /locus_tag="VLR27_00090"
     CDS             complement(17232..17735)
                     /locus_tag="VLR27_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01866.1"
                     /translation="MLRLLVVAEDPARERDMVWRAEAALEELSSVPEVEIVVSPRTDG
                     LLDVARLDAWLFTRPLPDRLIVVGRHPSCTPKELAFLDTMLDLRPDLSAVVAAETSGP
                     GVDALLAHGGIFRGGPLCQVGGFAGDGRRPVRARIEASGFRCSLLSDRPMMADGGLSR
                     GMHPVPA"
     gene            complement(17867..19330)
                     /locus_tag="VLR27_00095"
     CDS             complement(17867..19330)
                     /locus_tag="VLR27_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HSP01867.1"
                     /translation="MSGKHLVASGRRSFGQRALIAVGALLTFVLLLSAAGASYLNVRF
                     GQISRFDVAIDQVEAGKPRNYLLVGSDTRDGLDPDDPEDAQFFDDEGTFTEGTVQRTD
                     TIMILRVDPDAGEAYLLSLPRDLWVPIADTGERNRINVAYSRGRDVLVDTIRENFDIP
                     IHHYVEVDLVGFRGLVEAVGGVPVYFSEAVRDDYSGLRVDSPGCVNLDGQQALGFARS
                     RFLEIQDPDTGRWRTDPSGDLGRITRQQEFIRRAISKAVSKGLTNPVTLNELVGVGVE
                     FVGLDPTLSASDIVSLGRRFASFDAESLQTFSVPAEPFRTAGGASVLDLDEREAERIF
                     NVFRGLDPGELTPGLVEVTVLNGIGEQGLAGDVTAALDQIGFTTGTPGDTVEPETSTG
                     IYHAPGDEAAAALLARHVSGPVEFGVDEDLSSGEVVLVAGADFTTLHEQPSPTVPELP
                     STTGSTGGDEVTDMSSTTTTTEVVGYVPEPPPGSDCA"
     gene            complement(19385..20632)
                     /locus_tag="VLR27_00100"
     CDS             complement(19385..20632)
                     /locus_tag="VLR27_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP01868.1"
                     /translation="MDLTYPEEAETFRGEIRAWLEANLPEDWEDREMTAEEKAAFSEK
                     WNEKLFEGGWICASWPKEYGGKGLTTMQNVVLNEEFARVGAPMRGDFFGDTLVGPTIL
                     QWGTEEQKQHFLPGILQGKTAWCQGFSEPNAGSDLASLKCKAELDGDEWVINGQKVWT
                     TQAQHADYCFLLARTDPEAKKHAGISYLLVPMDQEGVEIRPIVQPDGSAEFNEVFFTD
                     ARCPKDNVVGGVNNGWKVAMTTLGFERGSSATTGHRRFEKELDQILDIAKENGAVDDP
                     MIRQRFVHQWSKIQIMRVNGLRTLSASLTGKKDLGVAALGATNKMFWSETHRDTMELA
                     IDVLGLHGQILTGDAEADFVPGIGKRDAAREYPVSPLQAQFFFSRSETIWGGTAEVQR
                     NIVGERVLGLPKEPKPENELPNG"
     gene            20717..>21073
                     /locus_tag="VLR27_00105"
     CDS             20717..>21073
                     /locus_tag="VLR27_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSP01869.1"
                     /translation="MALTDVVTGETARWTNQWLELKAEVIDTGLCTGCAGCVVACPHD
                     VIGYDHESGGYKPFHLEEELGPDDCIHGQKGCTSCTRACPRFRSWEPEADEHLFARYR
                     KDDELSGIYQDILLTKA"
BASE COUNT         3033 a         7517 c         7668 g         2855 t
ORIGIN      
        1 cttgccgccc agcgcgccga cgatcttctg cagctcgtcg cggtccttgc gttcgagggt
       61 ctcccgctcg agggcgttgg catccacgct catcgtgctc gtcgtgcttt cgtcgtgttc
      121 ggatggaggt ccgcgccggt cgcgcctggg acgggccgag gacggagagg aggttcgccc
      181 gtgagaccac gatcccctgg ccggctcctt gcgccggacc cgagccggtg tgccgcgcac
      241 gccagaccag gtcgcacggg acgatagccc tccgccggcg gcgcctccaa caccgccccg
      301 ggcgaccggc taccgggccg tcgtgtaccc ctcgagcagc ggcgggctgc cctcgagcag
      361 gttcaggagg gctcgctccc cccggccgta ccacttggag cgtcggctgg cgccccacac
      421 ctcggcgacc tggtcgtctt cggcgtcgaa caccgcgggg tgcacgtagc tgttgcgggc
      481 gacggtccgg gtgttgtgca gcaccgcagc ggccgcatcc gcgatctcgg gctccttctc
      541 ggccccctcg gcccgagcgg tgagggcggc ggcgctcccg ccccaggtcc gcaggtcctt
      601 gcagtcgagg cagccggcgc tgtgctcgtc gaggtaggcg ttggcgtcga ccgcgtcgat
      661 gaccgtgccg tcgacctcga agagccactc ctccgcgtcg gcctcgaggt cagcgatggc
      721 ggcggcgagg tcccggtcca cgatcgtcac gtcgtgctcg accccgccct tgccggtgaa
      781 cgacagccgc accgactggc gggacacgtc gatgttggag ggcgccagcg tggagatgcc
      841 ccggctcccg tggagctcga ccgagtccgg gttgccgatc cgctcgaggc tggtgtcgag
      901 caggcgcacc atcgtggcca gcgcccgggt gcagcggtcg ttcgcctcga ggtcgtcggc
      961 cacggcccgg cgcacgccgg cgagggactc ccccacctcc acgagccggt cgaagttggc
     1021 cgcggcccgc gcctcggtcc agcggtcgtg gtagcggtac tggcggcggc cggcgtcatc
     1081 ggtgcccatc gcctggaggt ggccatcggc ccggcggcag acccagacgt cggtccacgc
     1141 cggcggcagc acgagcgcct cgcagcgctc ccggttggcg cccttgacga ccgatccgtc
     1201 gggccaacgg aagctgaacc cccgaccctg gcggcggcgg acgatgccgg gctcctcggg
     1261 atcggcggtg cgcaggccga tggccgatgc gaagtggtcg tcggcgctgg tcatggccgc
     1321 ttccattcgc cagacgggcc cccgaccaga ccacccggct gcgacggggt ggttcagtgc
     1381 tggatcagcc ggcggacgag cgcctgcacg gcgggcagct cggcgctgac ctcctcgacg
     1441 cgctcctcgc gctcgtagag gtcggcgtag tgcggcggca gcggcgggcg caccccggtg
     1501 gcggactcga ccgcatcggg gaacttcgcc gggtgggcgg tggccaggca cacgaccggc
     1561 gcgtcgggca ggtcggcgac ccggtccgcg gcgccgacgc ccacagcgga gtgcgggtcg
     1621 agcagcacgc ccgagcgctc gtggtgctcg gcgatgacgg cacgcgtctc gtcgtcgtcg
     1681 aggcgggcgc cccaccagcc atgagggcgg ggtgcctcga cgtggccggt ggcccggtac
     1741 tccgacagca gctcggcggt ggcccggccg tcccgaccga gcaggtcgaa cagcagccgc
     1801 tcgaggttgg acgacacctg gatgtccatg ctcgggctga tcgtgggcac gaccggttcg
     1861 gcggtgatgg ccccggtctc gagccaccgg gtgagcacgt cgttggcgtt cgaggccacg
     1921 acgaagccgg ccacgtcgag gcccatctcc tgggcgaccc agccggccag cacgttgccg
     1981 aagttgccgg tggggacgga gaacaccggc gcctggccgg tctcggcccg cacggcccgg
     2041 gcggcggtga cgtagtacac gacctgggcc atcacccggc cccagttgat ggagttcatc
     2101 gccgacagct ggacctcgtc gcggaacagc cggtcggcga aggcggcctt cacgatgtcc
     2161 tggcagtcgt cgaagctgcc ggtcgccgcg acggcgtgca cgttcggcgc gtcgatcgtg
     2221 gtcatctggc ggcgctgcac gtcggagacg cgaccgccgt cagggaacag gacgacgcag
     2281 tcgagcgccg cccgcccgac ggtggcggcc atggcggcgg agccggtgtc gcccgaggtg
     2341 gccacgagga tcgtcgcccg ccgcccccgc tcctggaggt agtggtcgaa gagccgcccg
     2401 acgagctgga gggcgacgtc cttgaacgcc atcgtcgggc cgtggaacag ctcgaggagc
     2461 cagcgccgct cgtcgaggcg gaccagcggg cacacgtcgg ggtggtcgaa ggtggcgtag
     2521 gcgtcggcga cgatggcgtc gaaggtcgcc cgctcgatgg tgccctcgag gaagggccac
     2581 atgaccgccc cggcgacctc ggcgtaggac tcggcaccgt ccaggtcggg cagccggggg
     2641 aactgcaccg gcacgtacag gccaccgtcg ggcgccaggc cggtgagcat cgtgtcggcg
     2701 aagtccagct ggggggcgtc gccccgggtg gagacgtagc gcagcgtgct ggtggtggtc
     2761 gtcatgcgcc gagcacccgc agcaccgtgt tcacctggcc cacgtcgggg tgctcccgca
     2821 ggtcgtcgag ggtggcgcgc agatcggctt cgcgagccgt gtgggtgatg aacaggaccc
     2881 gggcatctgc gccgacgtcg atctgctcca tcgaccggat gctcacgtcg cgctggccga
     2941 agatgccggc gaccgcggcg agggcgccgg gccggtcgtg caggtcgagg ttcaggtagt
     3001 actccgaccg cagctgctcg atgggcacga tgtgcgccgg gtcggacgtg tagatgctgg
     3061 cgtgggtgcg cttgcgcagg ttccccgcgg catcgatgag gtcgcccagc accgcgctgg
     3121 ccgtcgggcc gccacccgca ccgcgcccgt agaacatgag gtcaccgacg gccgcgccct
     3181 cgacgaagat ggcgttgaac gagtcgcgga ccgacgcgag cgggtgggtc tccggcacca
     3241 gcgtggggtg gacccgggcc gcgatgccgt ccaccccgaa ggactccacg atggcgagca
     3301 gcttcacgac gtagccgagc ttggtggcct gctcgatgtc ggacgcaccg atgttggtga
     3361 tgccctccac gtagacgtcg tcggtgacga tccggctgcc gtaggcgatc gagccgatga
     3421 tcgccgcctt ggccgcggcg tcgtggccct cgacgtcggc ggtcgggtcg gcctccgcgt
     3481 agcccagcgc ctgggcctcg gccagcgcat cggcgtactc ggcgccgtcc tcggtcatct
     3541 tggtgaggat gtagttggtc gtcccgttga cgatgcccat cacccgggag atgtgctcac
     3601 cggcgaggga ctcccgcagc ggccggatga acgggatgcc gccggccacc gcggcctcga
     3661 agagcaggtc gaccccggcg gcgtcggcga tcgcgaagag gtcggcgccg gacccggcca
     3721 gcagcgcctt gttcgccgtg atcaccggct tcccggcggc gagcgccttg gccaccagcg
     3781 tcccggccgg ttcgagcccg cccatgagct cgatgacgac atcgacgtcg gggtcctcca
     3841 ccacggagag cgcgtcggtg gtcaggtgcg agggatcgat ctcggccgaa cgggccttgt
     3901 cgagcgagcg gaccgcgacc ggaccgagct cgaagcgcag gccggtacgg gcctcgatgt
     3961 cgtcaccgcg ggaggccagg agcccgacga gtgacccgcc gacgacgccg cagcccagca
     4021 gcccgacgcg gatcgtggtt ggtgccatgg gcggtcacgg tagcgagccc ccctcgtggc
     4081 ccccgcaccc gatcgggagc ccacgcccct agaagacgtc gttgcgcacc aggtcgtcga
     4141 aggactcgcg gcgcaccacc gaccgggcgt cgccgtcccg gcagaacacc acggcagggc
     4201 gcaggatctt gttgtagttc gaccccatgg agtgcccgta ggccccggtg accggcgtgg
     4261 ccaggacgtc gtcgaccgcc acgtcgtcgg gcacccatcc ctcgcgcacg agcaggtcgc
     4321 cggactcgca gtgcttgccc acgacacgca ccggcttggt gcgctcggcg tcggtggccc
     4381 ggggcaggaa cgtctcgtag ccggacccgt agagcaccgg gcgggggttg tccgacatgc
     4441 ccccgtcgac cgacaggaag gtgcgcacgc cgggcacgtc cttgatcgtg cccaccgtgt
     4501 agagcgtcac ggctgccgcg gccgcgatgg accgcccggg ctcggcggtg atcctcgcgg
     4561 tgatgccggc ctcgcgggct gcggcctgga tggcaccgcc ccagtcggcc atcgtcggtg
     4621 ccgtctctcc ctcgacgtag ggcacgccga gcccgccgcc gatggagaac tccggcaggt
     4681 cgtgggtggt cacgaagggg accatggcct cgaccgcgtc gtggaacgag tcggcgacga
     4741 agacctggga cccgatgtgc atgtggatcc cgacgaggtc gaccgcaggg gacgccgacg
     4801 cgagctcgat ggcccggccg gcagcaccgg atcgcaggcc gaacccgaac ttggagtcct
     4861 cctgcccggt catcacgaac tcgtgggtgt gggcctcgat gccgggggtg atccgcagca
     4921 ggaccttcgg cgcgtagccg tcgtcctggt ggaggcgggt gaggcgctcg agctcgtcga
     4981 agctgtccac cacgatccgg ccgacgccga cggtgcgggc gtaggccagc tcggccaggg
     5041 acttgttgtt gccgtgcagc acgagcttgt cggccgggac gccggcggtg agggcgacgt
     5101 gcatctcacc gccggtggcg acgtcgaggt ccatcccctc ctcgtgcacc agcttcgcca
     5161 tggccacgca caggaacgcc ttggtcgcgt aggcgacgcc gtcggggaac gcggcgacgg
     5221 cttcgcggca ccggttgcgc aggtgctgct cgtcgtacac gaacagcggc gtgccgaact
     5281 cggcggcgac ctcgagcagg tcgcatccac cgatcgagag gcgcccgtca gcgtcgatcg
     5341 ccgagtgctc cggcagcagg tgggtgggga gcggaccggc cactacatgg actccggcgc
     5401 cgacacgccg agcaggtcga gcccgatggc gaagccgacg cgggcggcgt cgaccaacca
     5461 gagccgggcc tgggtgagct cggccggtac gtcaccgatg acgtagcagt cgtggtagaa
     5521 gccgtggaac cggtcagcca gctcgcgcac ccagttggtg atggtgtgcg gggcccgctc
     5581 ccgggcggcg cggaccagca cctcgggcag ctccgacagc gtgcgcagca gctcgatctc
     5641 ccgctcgtgg gtgagcagcg acaggtcggc atcggccagc ggggcgcgga cgacgccggc
     5701 ggcggcggcc ttgcccatga tcgagtggat ccgggcgtgg gcgtactgca cgtagaagac
     5761 cgggttgtcg ttggaccggc tcgtgacgag gtcgaggtcg atcgcctgct ggctgtcgac
     5821 ggactggagc aggtaggtca gccgggccac gtcggcgccg acctcgtcga tgaggtcgcg
     5881 cagctcgatg atgtcgccgg cccgcttgga caggcgcacc tccttgccgt cccgctcgag
     5941 gcgcacgatc tgcacgatcg gcacctcgaa ctcgtcgggg tcgtggccca gcgcctcgat
     6001 gccggcccgc atccgcggca cgtagccgtg gtggtcggca ccccagacat cgatgagcag
     6061 gtcgaagccc cgggcgaact tgtcccggtg gtaggcgatg tcgggcagca ggtaggtcgg
     6121 gtcgccgtcg gacttgagca gcggccggtc cttgtcgtca ccgaagtcgg tggagcgcag
     6181 ccaggtggca ccgtccgcgt cgtaggcgtg gccccgctcg cggaggtcgg ccagggccgc
     6241 gtccatcgcc ccgctgtcga cgagcgcctg ctcggaggac cacacgtcga agtgcacgtg
     6301 gaaggcggcg aggacctcgc gctgatcggc cagcgcccgg tcgtagcccc aggcccgcac
     6361 cgcgtcgatg ccggcgtcgg cgacgtcgtc gggcatctcg gccgcccagt cggcgacgta
     6421 ctcgcctccg tagccgtcct ccggcacggg ctcgccccgc ttgcgggcga ggagggattc
     6481 ggcgtagcgg gccatctgga cgccgcggtc gttcaggtag aactcccgcg tcacgtcgaa
     6541 gccggtgcgc tccatgatcc gggccaccga gtcgccgtag gccgcaccgc ggccgtggcc
     6601 ggcgtgcagc ggaccgttgg ggttggccga gacgaactcg atgttcacct tggtgccacc
     6661 accgacgtcg ggccgggcgt agccgtcggt gccgccctcc accacctcga cgagcacgtc
     6721 gtgcagccac gacggggcga gccggaagtt cacgaacccg gggccggcga tctccaccgc
     6781 atcgacgtgg gcgatgtcgg cctgctcgag ggcggcgacc aggtcgccgg cgatctcgcg
     6841 cggcggacgg ccggcggcct tggccgatgc catcgcgatg ttgcaggacc agtccccgtg
     6901 ctcacggcgc gccggacgct ccatcgtcca ggtgacggcg gcggggtcga ggccgatgcc
     6961 ggcggctgcc gactcgactg cagcagcgag cgtgtcgcgg atcaacaggg ggctccaggt
     7021 gtcgtggggg gggtgtgcgg tgtcagccgt tgcgcagcag gacggcgttg acgcggtcga
     7081 tcaggtccag cggctcgaag ggcttggtga cgtagtcgtc ggcgccggcc tcgaggccca
     7141 tgcgcacgtc ggcgccctgg gccttggccg acagcaggat gaccgggatg gccttcgtgg
     7201 cggctgaggt cttcagcgcc tgcaccagct ccaagccgga catcttgggc atcatgacgt
     7261 cggagacgat cacgtcgggg cgggattcgc gagccacggc caggccctgc tcgccgtcct
     7321 cggcccgcag cacgatgtag ccctccatct cgaagttcac ctcgaggagc tggaggatcg
     7381 tggggtcgtc ctccaccacg agcacggtgg gcgcgggcat ggcgcgagac tacgggggcc
     7441 cgtgcgggac ggccgaagcg gatgcggcgc ccccgatcgc ccgcgctacg atccgagtcg
     7501 ctccgccctc gtagctcagc ggatagagca tcgccctccg gaggcgtgtg cgggcgttcg
     7561 agtcgccccg agggcaccac agatctgtgc agtgcgtcac gtccgtcggc ggtcgatcgt
     7621 gacggcttcg ccgccacagg gcaccgtgac aggggtcgcc tcgccttcgg cgcggcgccg
     7681 ggcagacgaa gggcctgcag cctgtctcaa cgtggcggcg agcgggtacc gacccgcgat
     7741 gaccacgacg ccgggtgaac ggttccgcca gcacgccgag gagtacagcg aggacgccga
     7801 cgacgagcgt ccgctcggcg cctacacggt gctgctggtc gcctacatca gcgtcgtcac
     7861 cgccctggtg gtcttcggac gcaagcggct gccgggcacc ctggcgccgt cggacctggc
     7921 cctcggggcc ttcgccacct tcatcggcac ccgcacgctc accaagcacc ccatcgccag
     7981 cccgatccgc atgccgttca cgaagttcgc cggcgtcagc ggtccgtccg agctggagga
     8041 ggacgtccgg gtgagcggct ggcgccacgc catcggcgag cttctcacct gcccgttctg
     8101 cctcagccag tggatggcga ccaccctcgt gaccggcgtg gtcgtcgccc cccgactcac
     8161 ccgcaccgtg atgtccgtcc tggccatcgt cggcgcgtcc gacttcctcc agctcgccta
     8221 cgtccgggcc cagaacctgg cgaccgactg agcgagggcg ggtcgcccta ccgccaggag
     8281 tccagcagca gccgggcctc ggtggcatcg gcgtccagcg ggaggccgta ccagatcggg
     8341 aagaggaacg cgagcatcgc gaccgctgcg gccacgacca cccagggggc gatcctcgcc
     8401 gcccgtccgg gcagggaccg ggtgccggcg accagggcga gcgccgcgaa ggggaccagc
     8461 ggcgccaggt agaagatgta gccccgcccc agcccgagcc aggggacgta cagcgacgcg
     8521 ccgagcgcga ggaaccccac gagggcgcgc tcccggcggc gcacgacctg ccagagcacc
     8581 aggggcagtg cggcgagcat cgtccaccac agcgccgggt tgccgaccat gaccacccgc
     8641 gccgtggtcc cctccggcac cacgcagggg gcgccatctg tggtggggca gcgctcgagg
     8701 tacgacagca cgggacgctc caggatcggc caggtcggcg ccgcggaccg gtacgggtgg
     8761 gtgtgctcca gcgctcggtg catgcggacc aggtcccggt ggtggaagac cagaccggcc
     8821 acccgctcgg tgaccggggc gtcgcagtcg agctccgggc aacgctgctc gtacaccttg
     8881 gactcggtgt agttcaggaa gaagccgccg tagctggcga cgtacacgac gaacggcacc
     8941 acgacgaggg gcaggacggt cgccacgccc ccctcgagca cggcacgcag cgtgccccgc
     9001 cccgggcggc gacggcgcac ctcggtccag ccgacgagcg ccacggcgac ggcgagcatc
     9061 ggcgccgccg accacttcgt gccgaccgcg agcccgaggg cgacgccggc gagccacagc
     9121 tcgggccccg ccggggtgaa gcgccgggtg accggggcgc gggcacggac ccggtggtac
     9181 cgcagcagga gcagcagcgc cagcgagacg aacagcgcca ggaccgcatc gagcatcgcg
     9241 atgcggctcg tcgtgatggc cagaccgtcg acggcgacga gcagcgccgc ggccgccgcc
     9301 agccagcggt cccgcaggag caggagcccg gccccgtagg tgacgagcac catcgccgtg
     9361 ccggccaccg cggagccgat gcgccagccg accgggtcga agccgaggac ggcgatgccg
     9421 gcagcgagca cccacttgcc gacgggcggg tgggcgggcc gctcgacctc cacgccccgc
     9481 tcgaccaggg cgttggcgtc gttcgcgtag aagctctcgt cgaagtggat gcgctcgatg
     9541 tggccgaggt ccaccaggcg caggagcgcc gccacagcca ggacgacgag ggggatcgca
     9601 acggcgaccc aacggcgacg agcgggcacg atcagttggc gaaccgcagg aacggaccga
     9661 aggggccccg ctcgaacgtg atggcctcgg ggtcggggta gccgtcgacc tcggtcgggt
     9721 cgatcagcac ccgctccagg cggaacgctc cctcggtccc ctcgaacgac aggcggtcga
     9781 tggcgacgag gcgggaaccg tcgaactgca gcagctgcat ctcgtagggg aggtctgcgg
     9841 actcggtgag cgcgccgacc ccggtggcac ccgcgctgcc ggagtgggcc acgaccgagc
     9901 cctcccggta ctcgacggcg aagcggtgcg tgtgcccggc gaggccgacg tcgaaggtgc
     9961 cgatcgccgc gtcgagctgg tccgagttgt gcacggcgac cacgtcgggg cgggtgagct
    10021 gcacccgtcg ggccagcgac tccgatgccc tggccagcag ctcgtcgtag tcgtcccgag
    10081 cgagctggtt gtcggcggtg aaggccggat cgcccatccc gaggatccgg acgtcgccca
    10141 cctccgccac gcccgggtcg agcacctcga cgccggcatc gacgagcgca cgccggatgc
    10201 tcgagtggtc gtggttgccg gggaccaggt agtacggcac gccgaagcgt gcgatgcgct
    10261 gcacgaccag ctgctcgacg ctggagccga agctggtgat gtcgccggtg tcgatgatcg
    10321 cgtcgacgtc gaagcgctct gcggtctgct ccaccagctc gatgcccacc gggttcgagt
    10381 gcaggtccga gacgtgcagc agggtggtgt cggtcagcga cgggccggtg ccctccaccg
    10441 atcggtagag gcccacgagc cgagccgaca gcgcctcgag ccgcgactcg accaccccga
    10501 cctcgtcgat gtggcgctgg acggtgttga cgatgtcggg ggcggcagcg agggaaccct
    10561 cgaaccgggg ctggtcgaag gccgtcggcg agaagctggc gaaggccatc gcaccggcga
    10621 ccacgacgaa gcccatcgac ccagcagtgg tcgcggcgac gtagcgcacc cggcgctgcg
    10681 ggagcaggag cccgaccacc acgccggcca ccagggcgac ccccaggctg cgcaggacga
    10741 ggctgcgcag cagcgggctc aggtcgtcgt cgatgtcggc tcggaggcgc tgcgccgggt
    10801 ccgagccgcg cgccagctca ccggccagcg cccccgcctg ggccaggtcg atgcggtcga
    10861 cgcgcacgtc gaacccgacc ggtccccggt ggctgtcggc cacgaggtgc ccgagcggcg
    10921 ggaggtcgac gttggtctcg ctggcgcgca cgcccacgtc gatggtgacc gaccccggac
    10981 ccagcgaccc gaccgtgtcg ggctggaggg cgaagctcag cgcgccggcc agggccccga
    11041 gcaccacgca ggcgcccagg gcgagggccg acgcacggtg gcgcctcacg aagttcccca
    11101 gctggtgcac ggccccctct gtacccgctg gccgggcgcg ctcgcatggc gcggtcacgc
    11161 cgacggcggg tcggctgggg accccgccgc gagcagggcg cccaggagct cgtcgagcgg
    11221 cgcacccttg cggacgtgcc cgtcgacctc gcccacctcc acggcccgcc gggccgcgtc
    11281 gtcgagcgcg gcggagaaga cgacgaccgc gggcgccgga tcggtggccc gcatctcggg
    11341 caccaggtcg agcccccacg tgccgcccag gcgcaggtcc atgagcacga catcgggacg
    11401 gtgctcggcc accgcggcca gcgtcgccgg tccgttgccg gcggtggcga cgacccgcag
    11461 ctgcggatga cgggtcagcg cgacgacgag ggtgtcgacg aggagcgcgt tgtcgtcggc
    11521 gatcacgacc cggacctcgc ggccgccacc gatgtcgtcc gtgctgtcca cgaccccatg
    11581 ctgcccgctg gccacggggc gtggtgggac ggctccggac gtgcgatcat cgcggcgtgt
    11641 tggacattcc ggacagcgcc gagcaggccc gcctcgggca gctgcgcgtc cacctggaga
    11701 cggtccagcg catcgccggc ctggggagct gggaggccga cgtccgcacc gaagagctca
    11761 cgtggtcggc caacgtctac cggatcctcg gcctgccgga gacggtcgag cccagctacg
    11821 accgcttcct gggggccgtg caccccgatg accgccaccg gatcgcacgc gcccacgccg
    11881 acgccatcga gggacggaac gcctactcgc tcgagcaccg catcgtccga cccgacggca
    11941 cgatccgcca cgtgcacgag gaagcgaccg ccgagcggga ggacggcgag gtcatccggc
    12001 tcctcgggac gatgcaggac atcaccgacc agatggcgct cacccgcagc ctcatcgaca
    12061 ccgagtcccg ccggcgcgag ctcctccacc gcctggtccg ggccagcgag cacgagcggg
    12121 cccagctcgc cggcgacctc cacgacggtc cgatccagat gctgaccgtg gcggcgatgc
    12181 gcctcgagca cctcggaatg gtcgagaccg acacgcccga ctggctgccc gaggccgtcc
    12241 agaccatgcg cgagaccgtg gtgcagctcc gcgacgtact cgtcgagctc cacccccgcg
    12301 ccggcgccgg cgccggcctc gaggccacgc tcacccagct ggccctcacc gtcgtgcccg
    12361 atctggacgt gcgcatcgag gtccggggcg acgccgacga cgccgagagc cgggccgtct
    12421 tcggcatcgt gcaagaggcc ttgtgggacg tgcgggaggg cgatcgctca cgtcacctcg
    12481 acatccgcgt cgacgtcggt gaggacgcgc tcgaggtcgt gctcaccggg gcccggcggg
    12541 ccgacggccg cggacccgag ctgctccagc gagccggcct gctcggcgtc cgcgagcgca
    12601 ccgagggggt cggcggtcgc tgcacggtcg acagcaccga cggcatccct gcggtccgct
    12661 gcacgctccc gcgcacggcc ccggagatcg cagcgccccg tgcctgagga gtccaccgag
    12721 ctgaaccggg tgctcgtgca cctgcagagc gcccaccggc tggccggcat cggcagctgg
    12781 gaggccgagg tcgctggccc ccctcgactg ctgtggtcgg cggaggtgca cgagatcgcc
    12841 gggctcccgc tcgaccagca gcccacgttc gaggacttcg tcgccatggt gcaccccgac
    12901 gatcgggcgc tcttcctcga tgcccgcgcc gccgccctca gcggcgagcg gccctacgac
    12961 atcgacctgc gggtgatcct cccggacggg ggccagcgcc agatccacat cgtggccgag
    13021 gtcgtccggg acgagggtgg caacgccgtc cggttggtcg gggccgtgca ggaccggacc
    13081 gaggagctcg gcgccctccg tcgactgcgg ctcaccgagg tggcgcggcg tgacctcctg
    13141 caacggctgc tggactccgc cgacttcgag cgcaaccgcc tcggccggca cctggagagc
    13201 gggccgatcg accggctcgt cgagatcgag cagcggctgg cccgggagat ccccgacggc
    13261 gcctcgccgc ggtggaccga tgcgctgatc tccgtccgca aggccatcga ctcgctccac
    13321 cagacgctca ccgacatccg ggccgagccg tccaccggcg acctggccca gatcctcgag
    13381 gacctcgccg cagagaccgc ccccggggtg cacatgtcga tcgaggtcga cgtcgccgcc
    13441 tcgctccgtc cgccggtcca ggccacgctg ctgcgagtcg tgcaggaggc gctacacaac
    13501 gtccgcaagc acgccggagc cgctcacgcc agcgttcgtt gcgtggtcga tggcagctgc
    13561 gtggacgtca cggtcgccga cgacgggcga gggttcgacg tcgacaccgt cgagcgcctg
    13621 cccggccacc tcggtctcgt ggccatgcgc gaccggctcg tgtcgctcgg cgggcacctc
    13681 gacgtccggt cccgtccggg ccgcacggtg gtggaggcac ggatccccct ggcgtaggcc
    13741 cgtgccggtg gcggtcagtc ccggccgacg gagacgagcc ccttggatgc cccgatcacc
    13801 acggcctcga gctgggagcg cgcgccgagc ttgcccagga tgttgcgcac gtggttccgg
    13861 acggtgtggt ggctgaggta cagcagctcg ctgatgtcga ccgtcgtgcg cccctccgcc
    13921 aggcagcgca gcacgtcgag ctcccgctcg gtgagcccga ccggcgggcc ggtgggacgg
    13981 ttcagccatc cgatcgccgc cgacagcgtc tgcgcatcga agatggcctc gccgctgtgc
    14041 acgcgcagca ccccggcggc caggtcgtcc agatcggcgg acttgcggac gaagccggag
    14101 cagcccgccg ccacggcctc ggcggccgcc tgcacgccct cgctggcggt gaggaggatc
    14161 gtcctgaccg gcggctcgtc ttcgtggatg ctgcgggcca gctcgatgcc ggtgccgtcg
    14221 ggcaggctgt agtcgagcac ggcgacatcg gggtgcagct cctgcaccat cgcgcgaccc
    14281 tcccgcaccg acgacgccga gccgaccacg tcgagctctg gcgaccgtcc gagcgcgagt
    14341 gcgatcgact cgcgcaccat ggcgtggtcg tcgacgatga ccacccggca cgctgcgctc
    14401 tgcctctccg tcacgacgtc tcgatcctgt ccagcatgtc cccgaccggc ggaccctatc
    14461 cagggacgag gcgacctcgg tccgatcagg acggataccg tcccgatgat ggacgacggc
    14521 cgggaccgcc acgcacgcca cgtgcggggt ctggtcgcag cgggcgacgt gctggcccgg
    14581 cccggcctct cggccggcag ccggttgcgc cggctgtgcg acatcgcaca tgcggcgctc
    14641 ggcgcgcacc ggaccgccgt cgtggtcacc gacgaccggg gagcgttcca catcgatcac
    14701 ctcgtcggcg cacccctcac cgaggcggag gagatgttcg ggctggcccg gctgggtgac
    14761 cacgagaccg tcgaacgcct caccaggcgc gaggccatcg tcgtggacga gggcgcccag
    14821 ctccccgggc ggggagccct cgtcgtgtgc cccatggccc tcgacggcga tctgtggggg
    14881 tgcttgtgcg tggaaggtgc acccgatggg cgtcagcacg tggacgacct gaccgtcgac
    14941 acgttgcgag gcctcgccaa catggcggcc cacgtcgtgc tggccgagca gctccgcatc
    15001 gaccgggccg agatcctgtc ccgggagctg ctcaccgagc agcgcaaccg tcgtcggctc
    15061 gccaaccgcc tccacgacgg cccccagcag atcctcgtgt cgctgcggat gcggctccag
    15121 atgctccagc gcgacgtctc cgacgagctc gaacgggagg tcggcctgct ggccgaccac
    15181 gtggagtccg ctcgtgacga gctgcgaggg ctgatcctcg acctcgacac gatgacgggg
    15241 cacgcgatgc tgtccaacgc cctgcgatcg ctgctcgaga ccaactcggc catggccggc
    15301 tggcagacct ccttccacgc cgaggagggc gttgacgacg tcaacgagga agcccagatc
    15361 accatcgagc gcgtggtgca ggaggccctg gccaacgcgc gccagcacgc tgaggcgtcc
    15421 agcgtccagc tccgcatcac cacctccggc gacatggtca ccgtggcggt catcgatgac
    15481 ggcgtcggca tcccgtcgca cgtctccacg gccagcaccg gaagcggcgg gatccgggcc
    15541 atgcacgagc ggatctggct cgtcggcggc accctccaca tcgagagcga cgccggcaca
    15601 ggcacccggg tcgtgatcac cgcccctcgc cacgcgtgac gccgcccggg gccgcgaggc
    15661 ctgggcgaga tgactcatct gcgccttgcg ccacgctgag cggccgccga cactcggatc
    15721 gtgcagaacc cccgcaccgc tcccccaggt gaccaggtcc ttgcccggcg cgtgaccagc
    15781 gtggccctga tcgaggacca gcaggtcctc gccggcgcca tccagcgctc gctgtcggtc
    15841 atgggcgacc tgcgcgtcgt cggttcggcg acgtcgttgg gcgagggggt cgccctcgtc
    15901 cgccagaccc agcccgacgt cctggtcagc gactaccgcc tcggagacgg agacgtgccg
    15961 agccgcctgg ccgagatcct ggccggatcg cccgagaccc gggtcctggt gttcacgggc
    16021 tggccggacg agaacagctt cctcgaggcc atggccgccg gtgcgtcggg gttcctcgac
    16081 aaggccgggc ccttcgagga gttcatcgat gccatccggc gggtcgccgc cggcgaggtc
    16141 gtcgtggccc cgcgcttcct gccagcgctc acccggcgag cgaccaacgc cgacagcacc
    16201 gccctgtccc ggcgagagct cgaggtgctc cagctgctgg ccgagggcct cagcacggcg
    16261 gaggtggccg accggttgat cctgtcggtg aacaccgtcc ggaaccacat cacccacgtg
    16321 ctgaccaagc tcggggtccg gtcccggctc gaggcggtca acgtcggcgt ccgccgcggg
    16381 ctgatccgct tcgacccccc ggcctgaccg ttcgcctcac tcgaccgcag cgaggtctcc
    16441 gagcaggtcg accggctggc gccactggcg ccgagcggtg aagcgcacga tggtgctggc
    16501 ggcgtggaac gcgccgaacg cgacggcgtg gggccgctcg gccaggtcga ggtcgaacgc
    16561 cggcaggcgc aggccggatg cgcggaaggc caccgactcg accatccccc accacagggc
    16621 gaagtcgacg ccttccagcg tcccgacgtg cggtggcagg tgctggacga gcagatcggc
    16681 cggcaccggg tagcgggacc ggccgcggag ccgctcgagc gcggacgctc cagcggccac
    16741 gaggacctcg atcgcctcgg cgatcaccag ctggtcgcgg atggcggcga gcagtcgaca
    16801 ggcctcggtg tggcgaccga ccagctcttc gtgcagcgcg tcgagcgcga ggtcggggcc
    16861 ggacatctcg ggcggcgcca accccggcac cgacgtcgat cccaggaccc acggcaccgc
    16921 cccccgccgg tagctgcgcc acatggaggg cacgtcgtcg gcacggtgca gggcgaagag
    16981 ctcaccgacc ccgggaaggg ccgacgtcca gcgcccggca gcggcgtggg cctcgtcgag
    17041 cgcttcggcc gccaccgcga gcaccgcgac gggctccgtc cgagcgaggg cgatggcgtc
    17101 gggcagaccc ggaaggcaac gcacctcatg gccggcggcg gccaggatgc ccaggacacc
    17161 ggcgtcgacg tcggggccgg ccacgacggc cagggtgccc cgcaccggag catgcgatcc
    17221 gtcggagatg ctcacgccgg caccgggtgc atcccccggg acaggccgcc gtccgccatc
    17281 atcgggcggt ccgacagcag ggagcaccgg aagccggagg cctcgatccg cgctcggacc
    17341 ggacggcggc cgtcgccggc gaacccgccc acctggcaca gggggccgcc ccggaagatg
    17401 ccgccgtggg cgagcagcgc gtcgacaccg ggccccgacg tctctgcggc caccacggcg
    17461 ctcaggtcgg gacggaggtc gagcatcgtg tccaggaacg ccagctcctt cggcgtgcag
    17521 gacgggtgcc gtcccacgac gatcagccgg tcgggcagcg ggcgggtgaa gagccaggcg
    17581 tcgaggcgag cgacgtcgag caacccgtcg gtgcgtgggg agaccacgat ctccacctcc
    17641 ggcaccgagg acagctcctc gagcgcggcc tcggcgcgcc acaccatgtc gcgctcacgt
    17701 gcggggtcct cggcgacgac gagcagtcgg agcatcggtg gtcctctcca tcgacggatg
    17761 ttgtcgaatg gatcggcaga tcccgacccg acgcgcagat gcgggcggac ctcgcgcacc
    17821 ggtcgatgtg gacgccatgg ggtggtgcac ctgcaccacc cgacgctcag gcgcagtcgg
    17881 agcccggcgg gggctcgggc acgtacccga cgacctccgt ggtcgtcgtg gtcgagctca
    17941 tgtccgtcac ctcgtcgcca ccggtggacc cggtggtcga gggcagctcg ggcacggtcg
    18001 gcgacggctg ctcgtgcagg gtggtgaagt cggcgccggc gaccagcacg acctccccgc
    18061 tcgagaggtc ctcgtccacg ccgaactcca ccgggcccga gacgtggcgg gcgagcagcg
    18121 cggcggcggc ctcgtcgccc ggggcgtggt agatccccgt gctggtctcg ggctcgaccg
    18181 tgtcgccggg ggtgccggtc gtgaacccga tctggtccag ggcggcggtg acgtcgccgg
    18241 ccaggccctg ctcgccgatg ccgttgagca cggtcacctc gaccaggccg ggggtgagct
    18301 ccccggggtc gaggccgcgg aagacgttga agatccgctc ggcctcgcgc tcgtcgaggt
    18361 cgaggaccga cgcaccgccg gcggtgcgga agggctcggc gggcacgctg aacgtctgga
    18421 gggactcggc atcgaaggac gcgaagcggc ggccgaggga gacgatgtcg ctggccgaga
    18481 gcgtggggtc gagcccgacg aactcgacgc cgacgccgac cagctcgttc agggtcacgg
    18541 ggttcgtgag gcccttggag acggccttgc tgatcgcccg ccggatgaac tcctgctggc
    18601 gggtgatccg cccgaggtcg ccgctggggt cggtgcgcca gcggccggtg tcagggtcct
    18661 ggatctcgag gaaccggctg cgggcgaagc ccagcgcctg ctgcccgtcc aggttcacgc
    18721 agccaggtga atcgacccgg agaccggagt agtcgtcgcg caccgcctcg ctgaagtaca
    18781 ccggcacccc gccgaccgcc tcgacgaggc cgcggaagcc gacgaggtcg acctcgacgt
    18841 agtggtggat cgggatgtcg aagttctccc ggatcgtgtc gaccagcaca tcccgaccgc
    18901 gggagtacgc gacgttgatg cggttgcgct cgccggtgtc cgcgatcggg acccacaggt
    18961 cccggggcag ggacagcagg taggcctcgc ccgcgtcggg gtcgacccgc aggatcatga
    19021 tggtgtcggt ccgctggacg gtgccctcgg tgaaggtgcc ctcgtcgtcg aagaactggg
    19081 cgtcctcggg gtcatcggga tcgagtccgt cccgggtgtc ggagccgacc agcaggtagt
    19141 tgcgtggctt ccccgcctcc acctggtcga tggcgacgtc gaagcgtgag atctgaccga
    19201 accggacgtt caggtacgaa gcgccggccg ccgacaggag cagcacgaag gtgagcagcg
    19261 cgccgaccgc gatgagggcg cgctggccga aggagcgccg cccggaggcg accaggtgct
    19321 tacccgacac cgcatcgacg gtagtggtgg gccttcgcgc gcacccagtc gcagccggtg
    19381 cccactagcc gttgggcagc tcgttctccg gcttgggctc cttgggcagg cccaggaccc
    19441 gctcccccac gatgttgcgc tgcacctcgg cggtgccgcc ccagatcgtc tcggagcggg
    19501 agaagaagaa ctgggcctgc agcggcgaga ccgggtactc ccgggcggcg tcgcgcttgc
    19561 cgatgccggg cacgaagtcg gcctcggcgt cccccgtgag gatctggccg tggaggccga
    19621 gcacgtcgat ggcgagctcc atggtgtccc ggtgcgtctc ggaccagaac atcttgttgg
    19681 tcgccccgag tgccgccacg ccgaggtcct tcttgccggt gagcgacgcc gacagcgtcc
    19741 gcaggccgtt gacccgcatg atctggatct tggaccactg gtgcacgaac cgctggcgga
    19801 tcatcgggtc gtcgacggcg ccgttctcct tggcgatgtc gaggatctgg tccagctcct
    19861 tctcgaagcg ccggtgcccg gtggtggccg acgagccgcg ctcgaagccg agggtggtca
    19921 tcgccacctt ccagccgttg ttgacgccgc cgaccacgtt gtccttgggg cagcgggcgt
    19981 cggtgaagaa gacctcgttg aactcggccg agccgtcggg ctggacgatc gggcggatct
    20041 ccacgccctc ttggtccatg ggcacgagca ggtaggagat gccggcgtgc ttcttggcct
    20101 cgggatccgt gcgggcgagg aggaagcagt agtcggcgtg ctgggcctgg gtggtccaga
    20161 ccttctggcc gttgatgacc cactcgtcgc cgtcgagctc ggccttgcac ttgaggctgg
    20221 cgaggtcgga gccggcgttg ggctcggaga agccctggca ccaggccgtc ttgccctgca
    20281 ggatcccggg caggaagtgc tgcttctgct cctcggtccc ccactgcagg atggtgggcc
    20341 cgacgagggt gtcaccgaag aagtcgccgc gcatcggggc accgactcgg gcgaactcct
    20401 cgttgagcac cacgttctgc atggtcgtca ggcccttgcc gccgtactcc ttgggccacg
    20461 acgcgcagat ccagccgccc tcgaagagct tctcgttcca cttctcggag aacgccgcct
    20521 tctcctcggc ggtcatctcc cggtcctccc agtcctcggg caggttcgcc tcgagccagg
    20581 cgcggatctc gccgcggaac gtctcggcct cttcgggata cgtcaggtcc atgcatccca
    20641 tggttgccgt ggcccgtggc gccgggcaac tcaccatggt tcggcctttg cacggtgatc
    20701 gccgtagtat ttcgccatgg ctctgaccga tgtcgtcacc ggagagaccg ctcgctggac
    20761 caaccagtgg ctcgagctga aggcagaggt catcgacacc ggcctgtgca ccggctgtgc
    20821 cggctgcgtg gtggcctgcc cccacgacgt gatcggctac gaccacgagt ccggcggcta
    20881 caagccgttc cacctcgaag aggagctcgg ccccgacgac tgcatccacg gtcagaaggg
    20941 ctgcacgtcc tgcacccggg cctgcccccg cttccgcagc tgggagcccg aggccgacga
    21001 gcacctcttc gcccggtacc gcaaggacga cgagctgtcc ggcatctacc aggacatcct
    21061 gctcaccaag gcc
//
LOCUS       DATISU010000002         8355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_792113, whole genome shotgun sequence.
ACCESSION   DATISU010000002 DATISU010000000
VERSION     DATISU010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792113"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VLR27_00110"
     CDS             complement(<1..507)
                     /locus_tag="VLR27_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HSP01870.1"
                     /translation="MVDSLAGVALGAGAGERLRPLSQLRPKVLCPVGDDPLIDHAIGR
                     LSSVVDDVAVNVHESQPALIRHLDHRVHLSLEQGEALGTAGGVANLRGWLDGRDVVVV
                     NGDTWCPGGLEHLVDGWDGTSIRILVAGDEPFGPRAALAASLMPWDVVQDLPVTPSGL
                     WEVCWRQAL"
     gene            506..868
                     /locus_tag="VLR27_00115"
     CDS             506..868
                     /locus_tag="VLR27_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01871.1"
                     /translation="METRRWINHSLPQTLGNAVLLLYISAAFDAVLGGYLFSPVGIAI
                     VAGKAAAGYGIANEKKWGYNLGLAMAFLPFLLRFLIGGFSLSSIVGPSIISLMFEILL
                     VALLLHKDSKEHQRIWFS"
     gene            967..2598
                     /locus_tag="VLR27_00120"
     CDS             967..2598
                     /locus_tag="VLR27_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HSP01872.1"
                     /translation="MDFNLADVYEAVADAIPDNDAIIWGERRWTYRQLDERANALANV
                     LHGAGHGARSSRAGLGGHETHQDHLAIYLYNGNEYLESMLGAFKARVAPFNVNYRYVE
                     EELLYLLDNCQANAIVFDSRFADTLAAVLPELPELRTLIQVGDGPLLEGAVAYEDVLA
                     TASTLAPSVERSPDDLYILCTGGTTGMPKGVLWRQADIFMGSMGGRDLSTGTEKGSRE
                     EIVDAAKNSGGAKVLPAPPFMHGAAQWVAFSGFNGGSSIVIQDDVVRLDPADVWRAVD
                     REGVNVMLIVGDAFGRPLVEELEQGDHDGSSLLAIVSGGAPLNSTLKDRFLAALPSVM
                     ILDAVGASETGSQMSHVSAAGAKSSTGRFTPGPSATVVSADLSTVLEPGHEGDGWLAQ
                     KGRVPLGYLGDADKTERTFPVIDGTRYAVPGDRARLTAEGEIELFGRDSVTINSGGEK
                     IFAEEVEQALAHHPDVFDVVVAGRPSDKWGNEVIAIVQLRDGATPDEQALRDEAATHV
                     ARYKLPKAFVFVDSVMRSPAGKADYRWAKEQALNV"
     gene            complement(2588..3070)
                     /locus_tag="VLR27_00125"
     CDS             complement(2588..3070)
                     /locus_tag="VLR27_00125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP01873.1"
                     /translation="MTVTHDLEACDRRIDDLESCERRVEARAAFTPGDDYRQVVDLVL
                     AAAAVLDPPVPRLRLDDLRLAVTEACSNAVKVHRPDALGDPIVVSCQIDDTCVHVEVR
                     DRGPGFDPTERDPLPEPEDPERLRHEHGLGVELMTLLADHVTFRPEEDGTVVVLCVRR
                     "
     gene            3266..4435
                     /locus_tag="VLR27_00130"
     CDS             3266..4435
                     /locus_tag="VLR27_00130"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HSP01874.1"
                     /translation="MEAPDGTRWIGVGTSSDRDARCAGIEAARAALLGRDACVLIAFA
                     SDDLDLEAFAAGLAEIAGDVPVVGCSTAGEIGPGGTSDGAAVVVAFGGPGFHARTALA
                     RSASADLRRAGADVTAAALPADGDHRAVVLLSDGLAGDQQEVVRGAHSVAGALIPLVG
                     GCAGDEMRMERTRQIYGTEVLTDAVIAIGLTSDAPIGVAAQHGWRRVGEPMLVTAADA
                     NQVRELDGRPALDVYVERLGIPPTVAENPTAFAAFALTHPLGLARRGGEEVRFIAGAD
                     LADRSLRCIAEVPVGALVQLMEGDAVSVLGATDAACEDAVAALGDHDPLGLVVFDCVA
                     RRAVLVGDGVDQEMDSIRSHANGAPIAGFYTYGEIARSVGIKGFHNQTLVVLALA"
     gene            4432..6183
                     /locus_tag="VLR27_00135"
     CDS             4432..6183
                     /locus_tag="VLR27_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSP01875.1"
                     /translation="MTGTPTSHVQQLTELLVQLSSTAEDVDALVQRALETTVDAVDAE
                     VAAVVRGDVVVASVGFERDRVPAPALRALAAGRRAELALGGDPVHLAALRLDDDGLLV
                     VGRREETFAPPEIALAQGMASALALCLRSADLVARERTARLRSDRQAREARLDPLTGL
                     PNRAVFFERLAASTTRARTDDSRLAVLFMDLDGFKHVNDTLGHDVGDELLRAVAGRLA
                     GAVRRTDLVARLGGDELAVLVEQVESTARVEAMADHLCDALAVPISVRGRMVSVRTSI
                     GIALGPHPGDDPQLLLRDADVAMYRAKSDRPGSHVVFDQPMRDAVTARAELADDLRHA
                     VGERQLDVVYQPVVELGTLRTVGVEALLRWDHPTKGTIAPTTFVPLAEELGLIFELGD
                     WVMDMACRDLVRLQQRGILDVNVALNVSSHQLHDSGFALRVRRLLATHGVSPTSLVLE
                     ITETAALSDDTVVFEQLESLHEMGIPIALDDFGTGYSSLSHLSRLPISILKIDRHFVS
                     RLLTSARHAALTESVVALGRRLDLDVVAEGIEDTDQLLALEAVGCPYGQGFLLGRPGP
                     LEDIEAGAATSTASSRC"
     gene            6491..6622
                     /locus_tag="VLR27_00140"
     CDS             6491..6622
                     /locus_tag="VLR27_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01876.1"
                     /translation="MRFPRRFREERAHAHAADDSRKLRELQRAYSYELYRNPSLPLR"
     gene            complement(6669..8078)
                     /locus_tag="VLR27_00145"
     CDS             complement(6669..8078)
                     /locus_tag="VLR27_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HSP01877.1"
                     /translation="MNAKAHFHVLGIPVRIEPFFWIAAVLLAYNLGDARLILMWVAVV
                     LVSVLVHELGHAVALKAFGQSSAIVLHGFGGLTFSQRKLDRARSIAVTLAGPLAALVL
                     LGIPAMLLRDSDYGVELSFDYQLSDQVFGIWPVIVFVAYVNIWWSIANLLPIRPLDGG
                     NVLTELVGIQPARLVSIVVGTAAAVWAYTHTDAFRYAAFFAGFLVLINVSEYRKARQG
                     VRAPSAFDVEGPPVPGGGGARPGEHGGPARARPEHPAPRRGGRPTGPPAARPDTAPAP
                     GLPGGIDAASGESMVWNLLRRGDVPGARRVLSRVNGAVSPFVPATVAVAAGEGTDALV
                     DAYLARPSGPANLVPASTVSDAGAAPELARRLVDAGPSGVEAAAGLQTHLHYADRFAD
                     AAAVGELVHGAAGASRAQVAFDTACAWSRAGDPERGLAWVSTALDDGFHAPGLLDGEP
                     DLEAVRARPGWPDVRARLT"
     gene            8156..>8355
                     /locus_tag="VLR27_00150"
     CDS             8156..>8355
                     /locus_tag="VLR27_00150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP01878.1"
                     /translation="MTTDAVGTHPLPDADHLPEGEEKARAVRAMFDTIAPRYDLVNRI
                     MTFRMDVGWRRRAIKALDLGARS"
BASE COUNT         1198 a         3129 c         2913 g         1115 t
ORIGIN      
        1 gagcgcctgg cgccagcaca cctcccacag ccccgacggg gtgacgggca ggtcctgcac
       61 gacgtcccac ggcatcaacg acgcggccag cgcggcacgg ggcccgaacg gttcgtcgcc
      121 ggcgacgagg atgcggatgg atgtgccgtc ccacccgtcg acgaggtgct cgagcccgcc
      181 ggggcaccag gtgtcgccgt tgacgaccac gacgtcccgg ccgtccagcc agccccgcag
      241 gttggccacg ccaccggccg tgccgagcgc ctccccctgc tcgagcgaga gatggacgcg
      301 gtggtcgagg tgccggatga gcgccggctg gctctcgtgc acgttcaccg cgacatcgtc
      361 gacgaccgag gacaaccggc cgatggcgtg atcgatcagc ggatcgtcgc ccaccgggca
      421 cagcaccttg ggccgcagct gtgacagcgg tcgcagccgc tcgcccgctc ctgcgccgag
      481 cgcgacacct gcgagactgt caaccatgga gacccgccgg tggatcaatc actcgctgcc
      541 ccagacgctc ggtaacgcgg tcctcctgtt gtacatcagc gcggccttcg acgccgtgct
      601 cggcggctac ctcttctcgc ccgtcggcat cgccatcgtc gccggcaagg ccgcggcggg
      661 ctacggcatc gccaacgaga agaagtgggg ctacaacctc ggcctcgcca tggcgttcct
      721 gccgttcctc ctgcgcttcc tcatcggtgg gttcagcctc agctcgatcg tcggcccgag
      781 catcatcagc ctgatgttcg agatcctcct cgtcgctctg ctgctccaca aggactccaa
      841 ggagcaccag cgcatctggt tcagctgacc cggtcggttc cggcggaccg ccgattggga
      901 agttgaccga tcggtcaata gcgtccctgc gaccgattcc gaggtgctgc agggggtagc
      961 tggcagatgg acttcaacct ggccgacgtc tacgaggcgg tcgccgatgc gatccccgac
     1021 aacgacgcga tcatctgggg agagcgacgc tggacctacc ggcagctgga cgagcgggcg
     1081 aacgcgctcg ccaacgtcct gcacggcgcc ggccacggcg cccgcagctc ccgcgccggt
     1141 ctcggcggtc acgagaccca ccaggaccac ctggccatct acctctacaa cggcaacgag
     1201 tacctcgagt ccatgctcgg ggcgttcaag gcccgggtgg cgccgttcaa cgtcaactac
     1261 cgctacgtcg aggaggagct gctctacctg ctcgacaact gccaggcgaa cgccatcgtg
     1321 ttcgacagcc gcttcgccga caccctcgcc gccgtcctcc cggagctgcc cgagctccgc
     1381 accctgatcc aggtcggtga cggcccgctg ctcgagggcg ccgtggccta cgaggacgtg
     1441 ctcgccaccg cctcgacgct ggccccctcg gtcgagcgct cccccgacga cctctacatc
     1501 ctctgcaccg gcggcaccac cggcatgccc aagggcgtcc tgtggcgcca ggccgacatc
     1561 ttcatgggct cgatgggcgg gcgcgacctg tccaccggca ccgagaaggg ctcgcgcgag
     1621 gagatcgtcg acgcggccaa gaacagcggt ggcgccaagg tgctgcccgc cccgcccttc
     1681 atgcacggtg ccgcccagtg ggtggcgttc tcgggcttca acggcgggtc gagcatcgtc
     1741 atccaggacg acgtcgtccg cctcgacccg gccgacgtgt ggcgcgcggt cgaccgcgag
     1801 ggcgtcaacg tcatgctcat cgtcggcgac gcgttcggac ggcccctggt cgaggagctc
     1861 gagcagggcg accacgacgg ctcgtccctg ctggcgatcg tgtccggcgg cgcaccgctg
     1921 aactcgacgc tcaaggaccg cttcctcgcc gcgctcccct cggtgatgat cctcgacgcc
     1981 gtcggcgcgt cggagaccgg ctcgcagatg agccacgtgt ctgccgccgg cgccaagtcc
     2041 tccaccggcc gtttcacccc tggcccgagc gccaccgtcg tgtcggctga cctctcgacg
     2101 gtgctcgagc cggggcacga gggcgacggc tggctggccc agaaggggcg cgtgcccctg
     2161 ggctacctgg gcgacgccga caagaccgag cggaccttcc ccgtcatcga cggcacccgc
     2221 tacgccgtgc ccggcgatcg cgcccgcctg accgccgagg gcgagatcga gctgttcgga
     2281 cgcgactcgg tcacgatcaa ctcgggtggc gagaagatct tcgccgaaga ggtcgagcag
     2341 gcgctcgccc accaccccga cgtcttcgac gtggtcgtcg ccggccgtcc cagcgacaag
     2401 tggggcaacg aggtcatcgc catcgtgcag ctgcgcgacg gcgcgacgcc cgacgagcag
     2461 gccctgcggg acgaagccgc gacccacgtc gcccgctaca agctgcccaa ggcctttgtg
     2521 ttcgtcgact ccgtcatgcg ctcccctgcc ggcaaggccg actaccgctg ggccaaggag
     2581 caggcgctca acgtctgacg cagagcacca ccacggtccc gtcctcttcg ggtcggaacg
     2641 tgacgtggtc cgcgagcagg gtcatcagct cgacaccgag gccgtgctcg tggcgcaggc
     2701 gctcggggtc ctcgggctcc ggcagcggat cccgctcggt cgggtcgaag cccggcccgc
     2761 ggtcgcggac ctcgacgtgc acgcaggtgt cgtcgatctg gcacgagacc acgatcggat
     2821 cgccgagcgc gtcgggccgg tggaccttca ccgcgttgct gcacgcctcg gtcaccgcga
     2881 ggcggaggtc gtccagccgc agccgcggca ccgggggatc gagcacggcg gcggccgcca
     2941 gcaccaggtc cacgacctgc cggtagtcgt cgccgggggt gaaggcggcc cgggcctcga
     3001 cccggcgctc gcacgactcg aggtcgtcga tgcgcctgtc gcatgcctcg agatcgtggg
     3061 tcaccgtcac catcacccct gggcgttcgg tccgctgctg cctgcgatcc gtacccatgg
     3121 gcatcggcgg taggccggga cgaagtgcgc atctcgcccc ttctccggtg gggtttcggg
     3181 caaagcggac gagccggcag ggaacgccgg agaccgatga tcgcgaagcc tccagatcgg
     3241 gggtcggcct gccgatgggg aaggggtgga ggcaccagat ggcacccggt ggatcggcgt
     3301 cggcacgtcg agcgatcggg acgcgaggtg cgccggcatc gaagccgccc gggcggcgct
     3361 gctcggacga gacgcgtgcg tgctgatcgc gttcgcctcc gacgacctcg atctcgaggc
     3421 cttcgccgcc gggctcgccg agatcgccgg cgacgtcccc gtcgtcggct gctcgacggc
     3481 cggcgagatc ggcccgggcg gcacctccga cggcgcggcc gtggtcgtcg cattcggcgg
     3541 accgggcttc cacgcccgca ccgccctcgc ccggagcgcg tccgccgacc tgcgtcgggc
     3601 cggcgctgac gtgacggcag ccgcgctgcc ggcagacggc gaccaccgtg cggtcgtcct
     3661 gctgagcgat ggcctcgccg gcgaccagca ggaggtggtg cgaggcgcgc actcggtggc
     3721 gggggcactg atcccgctgg tcggcggctg cgccggcgac gagatgcgca tggagcgcac
     3781 ccgccagatc tacggcacgg aggtcctcac cgacgccgtg atcgccatcg gcctcacctc
     3841 cgacgcaccc atcggcgtcg ccgcgcagca cgggtggcgt cgcgtcgggg agcccatgct
     3901 cgtcacggcg gcggacgcga accaggtgcg agagctcgac gggcgcccgg cgctcgacgt
     3961 gtacgtggag cgcctcggga tccccccgac ggtggccgag aacccgacgg cgttcgccgc
     4021 cttcgccctc acccacccgc tggggttggc ccgacgaggc ggcgaggagg tccggttcat
     4081 cgccggggcg gacctggccg accgctccct gcgctgcatc gccgaggtgc ccgtcggcgc
     4141 cctcgtgcag ctgatggagg gcgacgccgt gtccgtgctc ggcgccacgg acgcggcgtg
     4201 cgaggacgcc gtggccgcgc tcggcgacca cgacccgctc ggcctcgtcg tgttcgactg
     4261 cgtggcccgc cgggccgtgc tggtcggcga cggcgtggac caggagatgg actcgatccg
     4321 ctcgcacgcc aacggggcac cgatcgcggg cttctacacc tacggggaga tcgctcgcag
     4381 cgtcggcatc aagggcttcc acaaccagac actcgtcgtc ctcgcgctgg catgaccggc
     4441 acccccacct cccacgtcca acagctcacc gagctcctcg tccagctcag cagcacggct
     4501 gaggacgtcg acgcgctcgt gcagcgtgcg ctggagacca ccgtcgacgc ggtcgacgcc
     4561 gaggtggctg cggtcgtccg aggcgacgtg gtcgtggcat ccgtcgggtt cgagcgcgac
     4621 cgcgtgcctg cgccggcgct gcgcgccctc gccgccggcc gccgtgccga gctggcgctc
     4681 ggcggcgatc ccgtccacct cgcggcactg cgcctggacg acgacggcct cctcgtggtc
     4741 gggcggcggg aggagacctt cgctcccccg gagatcgccc tcgcccaggg gatggccagc
     4801 gcgctcgccc tgtgcctgcg atcggccgac ctcgtcgccc gcgagcggac ggcgcgcctg
     4861 cgcagcgacc gtcaagcacg cgaggcccgg ctcgatccgc tcaccggcct ccccaaccgg
     4921 gccgtgttct tcgagcgcct cgccgcatcc acgacacgcg cccggaccga cgactcccgc
     4981 ctcgccgtgc tgttcatgga tctcgacggc ttcaagcacg tcaacgacac gctcggccac
     5041 gacgtcggcg acgagctgct gcgtgccgtc gcgggacgcc tggccggcgc ggtccgccgc
     5101 accgacctgg tcgcccggct cggcggtgac gagttagccg tgctggtcga gcaggtcgag
     5161 agcacggcgc gggtcgaggc gatggccgac cacctctgcg atgccctggc cgtgccgatc
     5221 tcggtccgcg gccggatggt gtcggtccgg accagcatcg gcatcgccct cggtccgcac
     5281 ccgggcgacg atccccagct gctgctgcgg gacgcggacg tggccatgta ccgcgccaag
     5341 tccgaccgtc ccggcagcca cgtcgtgttc gaccagccga tgcgagacgc ggtgaccgcc
     5401 cgagccgagc tcgccgacga cctccggcac gccgtcggcg aacgccaact cgacgtcgtc
     5461 taccagcccg tcgtcgagct cgggacgctg cggaccgtcg gtgtcgaggc cctcctgcgg
     5521 tgggaccacc ccacgaaggg caccatcgcc cccaccacgt tcgtgccgct cgccgaggag
     5581 ctcggcctca tcttcgagct cggcgactgg gtcatggaca tggcgtgccg cgacctcgtg
     5641 cgcctgcagc agcgcgggat cctcgacgtc aacgtcgcgc tcaacgtgtc cagccaccag
     5701 ctgcacgaca gcgggttcgc gctgcgcgtc cgtcgactgc tcgcgaccca cggcgtctcg
     5761 ccgaccagcc tcgtgctcga gatcaccgag acggcggccc tcagcgatga caccgtcgtg
     5821 ttcgagcagc tcgagtccct ccacgagatg ggcatcccga tcgcgctcga cgacttcggg
     5881 accgggtact cgtcgttgag tcacctgagc cgcctcccga tctccatcct gaagatcgac
     5941 cggcacttcg tcagccggct cctgaccagt gcccggcacg ccgcgctgac cgagagcgtg
     6001 gtggccctgg gccgtcgcct cgacctcgac gtggtcgcgg agggcatcga ggacaccgac
     6061 cagctcctcg ccctggaggc cgtgggctgc ccgtacggcc agggcttcct gctgggccgg
     6121 ccgggaccgc tcgaggacat cgaggccggc gcagccacct ccaccgcctc atctcgctgc
     6181 tgatcggaaa ttcgcttgcc acgccggcca cggcgcgatc ggatggcgcc gtcctgcccc
     6241 gccgagtgag aggagcgccc atgctgcagc acatgatcca gcccgtcgct cccacgctcg
     6301 tgggcagcac cgggggcttc gacccccgca ccggcgccga cgcccgctga ccgccgcccg
     6361 tcgacccgac ggtcaccgcg cgctgcgccc accccaccgg tccgccagct cggctgcgga
     6421 ccctgatcag acgtggcgcg cccggcgcgc cccgatgatg caccacccca cccggaagga
     6481 ggtgacgacg gtgcgattcc cacgacgctt ccgcgaggag cgggcccacg cccacgccgc
     6541 ggatgactcc agaaagctgc gtgagctgca gcgggcctac agctacgagc tgtaccgcaa
     6601 cccctcgctg ccgctgcgat gagcagcgac gacccgggcc cggcacccag gtgccgggcc
     6661 cgttcgcgtc aggtgagacg ggcgcgcacg tccggccacc ccggtcgggc ccgcaccgcc
     6721 tcgaggtcgg gttcgccgtc gagcaggccc ggggcgtgga acccgtcgtc cagggcggtc
     6781 gacacccagg ccagaccccg ctcggggtcg ccggcgcggg accacgcgca ggcggtgtcg
     6841 aaggcgacct gggcgcgcga cgcgccggcc gcgccgtgca ccagctcacc caccgcggcc
     6901 gcgtcggcga agcgatcggc gtagtggagg tgcgtctgca ggccggcggc ggcctcgacg
     6961 cccgaggggc cggcgtccac gaggcgccgg gcgagctcgg gtgcggcgcc ggcgtcggag
     7021 accgtggatg ccggcaccag gttggccgga ccggacggcc gggccaggta ggcatcgacc
     7081 aaggcgtcgg tcccctcgcc ggcggcgacc gccacggtgg cggggacgaa cggcgagacg
     7141 gcgccgttca cccgggacag cacccgccgg gccccgggca catcgccccg gcgcaggagg
     7201 ttccacacca tcgactcccc cgaggcggca tcgatgcccc ccgggagccc gggagcgggt
     7261 gcggtgtcgg gccgggcggc cggcggtccg gtgggacgcc caccccgtcg gggcgcgggg
     7321 tgctcgggcc gggcacgggc cggaccaccg tgctcgccgg gccgggcgcc gccgcccccg
     7381 gggacgggcg ggccctcgac gtcgaacgcg gagggagccc ggaccccctg gcgtgccttg
     7441 cggtactcgc tgacgttgat caggaccagg aagccggcga agaacgcggc gtagcggaag
     7501 gcatcggtgt gcgtgtaggc ccacaccgcg gcggcggtgc cgaccacgat cgacacgagt
     7561 cgagcgggct ggatcccgac cagctcggtg agcacgttgc caccgtcgag cggtcggatc
     7621 ggcagcaggt tggcgatcga ccaccagatg ttcacgtacg cgacgaacac gatcaccggc
     7681 cagatcccga agacctggtc gctcagctgg tagtcgaagg acagctcgac gccgtagtcg
     7741 ctgtcgcgca gcagcatcgc cgggatgccg agcagcacga gcgcggccag cgggccggcg
     7801 agggtcaccg cgatgctgcg ggcccggtcg agcttgcgct gggagaacgt gaggccaccg
     7861 aagccgtgga gcacgatcgc cgacgactgc ccgaacgcct tcagcgcaac ggcgtggccg
     7921 agctcgtgga ccagcaccga cacgaggacg accgccaccc acatcaggat cagccgggca
     7981 tcgccgaggt tgtaggcgag cagcacggcg gcgatccaga agaacggctc gatccgcacc
     8041 gggatgccga gcacgtggaa gtgcgccttg gcgttcacct cgtgatgcta cgcagcgccg
     8101 ggcgtcaggc ccggcgcgct gcgtgcccgc ccacgtgccc ccgtatcgtt gccctgtgac
     8161 cacggacgct gtcggaaccc acccgctgcc cgacgccgac cacctgcccg agggcgagga
     8221 gaaggcacgg gccgtccggg cgatgttcga cacgatcgcc ccccgctacg acctggtgaa
     8281 ccggatcatg acgttccgga tggacgtggg ctggcgccgg cgagcgatca aggcactgga
     8341 cctcggcgcc cgctc
//
LOCUS       DATISU010000003        34089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_698952, whole genome shotgun sequence.
ACCESSION   DATISU010000003 DATISU010000000
VERSION     DATISU010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 34089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34089
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698952"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VLR27_00155"
     CDS             <1..202
                     /locus_tag="VLR27_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HSP01879.1"
                     /translation="KVFTEIQRLGQVSDDEMAKVFNMGIGMIAVVPAEEVYAALDVLR
                     TAGHRAVQIGTVEPGSGLVRYA"
     gene            234..737
                     /locus_tag="VLR27_00160"
     CDS             234..737
                     /locus_tag="VLR27_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01880.1"
                     /translation="MRTVVRTVSWVLLFAALGAAGASWFTPTPKLEADHASDLALGAL
                     REAEVDVERVKPPVRMVHETRDDVLVDAWRVVVDVQAQGAVEEVELRVQESAGQLVFV
                     DDLIGADGTERLLSEEQFEVMSQHRDDTLADRWVLRNAMAAVAAIGIAATAYILATRS
                     DPLWSAR"
     gene            734..1315
                     /gene="pyrE"
                     /locus_tag="VLR27_00165"
     CDS             734..1315
                     /gene="pyrE"
                     /locus_tag="VLR27_00165"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HSP01881.1"
                     /translation="MTPSRTVPAEAHAALVAHLKAHAIREGDFVLKSGKPTTWFCDAK
                     QTTCRPDGMLLVADAALAVLPDDVTAIGGLTMGADAVAFGVAAIAATRGRELRAFSVR
                     KEVKDHGGGGRVAGALEPGDRVVIVEDAVTRGVSMLEAADVIAAEVGAEAVLLMPVVD
                     RGGTVAAMAAERGLPLQALVTAPDLGFDYEGPL"
     gene            complement(1333..1590)
                     /locus_tag="VLR27_00170"
     CDS             complement(1333..1590)
                     /locus_tag="VLR27_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01882.1"
                     /translation="MFATASHRPSSAHPDDADARRRARIGFALSHPTVVIGLAMGADA
                     TTGIIGAAILALHGLVELQWHLTDRLEPAGVPAASLYSGPS"
     gene            complement(1661..2782)
                     /locus_tag="VLR27_00175"
     CDS             complement(1661..2782)
                     /locus_tag="VLR27_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HSP01883.1"
                     /translation="MTAASTVFETMDAVDGLPDAGDFEQVAFCHHRPSGLRAIVAIHS
                     TVLGPALGGTRFWPYDTETEALTDACRLARGMTYKHAAAGLDQGGGKAVIWGDPATIR
                     SEALIRAYGRFVHGLAGRYLTAEDVGTTQADMDLIRRETPHVTGVSPSLGGSGDPSPA
                     TAWGVLWALRAAAEVRWGSPDLSGRHVTVSGVGKVGRALVDHLVDEGATVTVADIRDD
                     AVRAVHDDHPEARQRLRTVEPDQAHAVDCDVFAPCALGAVLDERTIPELRCEVVVGSA
                     NNQLATPEDDQRLVDHDVLYVPDYVANAGGVINIAEEPAGYDQTRAWARIAGIHETVL
                     AVLERARTQSIPPGEAADRHAEDRIESVARVARLRTGRP"
     gene            2946..3152
                     /locus_tag="VLR27_00180"
     CDS             2946..3152
                     /locus_tag="VLR27_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HSP01884.1"
                     /translation="MADQEDLDTPEALLTPSEVAAMFRVNPKTVTRWARAGKLNAIRT
                     LGGHRRFKASEIRACLEEMSQSEV"
     gene            3199..4599
                     /locus_tag="VLR27_00185"
     CDS             3199..4599
                     /locus_tag="VLR27_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HSP01885.1"
                     /translation="MATPTAAFSLHLRVRLENTPGSLGRLAVAIGDAGGNITQLEGFD
                     VRGEYLDEDIVVNCISEAHQEEVKAAISGLDGVEITTCKDRTFEMHEGGKIEVLARMP
                     IRDADDLSMAYTPGVARVCMAIHADRERSRDLTIRKNTVAIVSDGTAVLGLGDIGPHA
                     AMPVMEGKALLFKNFAGVDAFPVCIDTTDTEEIIATVERLAPTFGGINLEDIAAPKCF
                     EIEERLKASLDIPVFHDDQHGTAVVVLAALQNALKLTDKKMSDLRVVIAGVGAAGVAC
                     GKILLEAGVAEIIGADRKGAIWEGRDDLNPAKQWFAEHTNRDRRKGAVGELLAGGDVF
                     IGVAGPGIIDPTDLDRMNTDPFVFALANPDPEIRPEQAKGRAKIIATGRSDFPNQINN
                     VLAFPGIFRGALDAKATDITEGMKLAAAEAIADAIPMSELRPDFIIPSVFDATVAPRV
                     AAAVAEAAVAEGVCRD"
     gene            4617..4895
                     /locus_tag="VLR27_00190"
     CDS             4617..4895
                     /locus_tag="VLR27_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01886.1"
                     /translation="MTDRGPDELDVDLRWSESDEPDAGPVELTWNRPATGEERSAATG
                     TVRRGVRLRPGASRTTSALRAEVDELRAEVERLRAELDALRAGLEQSG"
     gene            complement(4909..5643)
                     /locus_tag="VLR27_00195"
     CDS             complement(4909..5643)
                     /locus_tag="VLR27_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HSP01887.1"
                     /translation="MGADDLARRFLERSLAAVLLLLAVPLIALTAAVSFACYRAWPFF
                     VQDRVGLGGQTLRFVKVRSLPPDTARYADKYSLQASRIPAVMRTVRRLHLDELPQLLH
                     VARGEMAFVGPRPEMPNLHARLPEPFASERVSVRPGITGLWQISPHCHQLIGERTEYD
                     RLYIWHRTATFDAWILLRTLRKMSLGRTTQLYEVPHRLVRHAPSPAPVFPPIAIAERP
                     APSLLDAPPVHPNPATAALAVAGSAD"
     gene            complement(5765..6535)
                     /locus_tag="VLR27_00200"
     CDS             complement(5765..6535)
                     /locus_tag="VLR27_00200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSP01888.1"
                     /translation="MHPVDGVVVTHGTNDDLTSCITSVSRDVRSLVIVDNLPPTPLPD
                     VPNGVVVTNPQPRSFAENVNLAVARTEAPYVAVVNPDCVPDPGAISRLCAFLDDHPRA
                     GIVAPQLRWPDGRRQPTGRSFPTVGATLVRRTPLRLLFPPFDLQRRHYRLGDQADPWP
                     ADWLLGAFWVVRRRAFDEVGGMDERYPMYVEDIDLCARLRAAGHERWVLPEAGAVHEY
                     QAVIDRRFLSRRNVWHLRGMARFARKHPALLVRPDRAA"
     gene            complement(6522..7691)
                     /locus_tag="VLR27_00205"
     CDS             complement(6522..7691)
                     /locus_tag="VLR27_00205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSP01889.1"
                     /translation="MTATPKPRVLVIGQVPPPMSGQHVMVQRLLDALRAEGTWEVRHV
                     SPRFSESIGSMRHVSLGKVVTLLATMVALVRERRHGRADVAVYPVGGVAPQSSLRDAA
                     ILPFVRLAARRTVLHMHGAGHAAHWRPMRWWMRLARTAYRRADRAVVMTQFNREDPTF
                     LGVAETSVVPHPIPDRAGTRRPVDGADRGALHLLYVGHLGPQRGTPALLGALGDAASR
                     TDVALHLHLVGDCLEPYTVESLRADIASVPEPATVHWDPELRGDATWEAFAGADCFVF
                     PSVFPCESFGLVLAEALMFALPIVVTEWRGNLDVVTEACDRVVVQPGGSADDLRRSLA
                     DGLVEAASRWSPGEARSERNRREYERRFADDLRWGMLDVVAELAGSREGIVDAPR"
     gene            complement(7688..9148)
                     /locus_tag="VLR27_00210"
     CDS             complement(7688..9148)
                     /locus_tag="VLR27_00210"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HSP01890.1"
                     /translation="MRSRPPAFVASGALLGVLAIGTAARVGGAIAIIVGGALVIGDVV
                     LLRWHRHGRSTPTPTPAGDVLDDRVLSAIAASWVVVGLLPMHDIGRQSASAAVSTLTL
                     QTGLELLIYGAAGAVALLLLLAVVPWQPVGAPITLVPVWVLASAAWAAAPAYALARGA
                     EYVALLLLAVVTAGIGWRSRDALDRMLGLVLRWLVLATLALIAAGVALGPMFVVVTAA
                     NRDRFTWIGAHPTESGFMLGTALLVLLATDARRLRVPAWFRAGAVLAVAVALYQNQTR
                     TVLAGVVVGGLALLLLWVRSGSGAAWFATYATGALGVVAALFVGGAISSYILRGGDVS
                     RLTTLNGRTDLWDVGLAALDGPTDWLVGLGYGATRTVFIGDFAFAGNAHSSLLGTLVG
                     LGLVGAALLVAALVRAGSLLVSSGLLRVDAGRALLAMFLFSVVGAVSSDGLAEPHLAL
                     TVLFLVTGAAQGWRAIPRTDTTERGADLHQRAGAAT"
     gene            9252..10430
                     /locus_tag="VLR27_00215"
     CDS             9252..10430
                     /locus_tag="VLR27_00215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSP01891.1"
                     /translation="MRIALVTLQYPPTGVGGIGTYVETLASALAGAGHEVTVVCSARD
                     QRRSSAVEGGVRVERFSTLGPGWLRDRLVAPHQALRVRILHALSSAWALWRVRSRFDV
                     VEVPEWKAQGLLLRFVRRGAVVVHLHLGFELQAAWNGATASRGWRISFALERWTARLA
                     RVRTATSEQTRRLPDGSTWLADEEVEVVAPPLRSGPWRSCPPVQDTEPVVLFVGRLER
                     RKAPEVLVEALGSIAAEIPGCRVVFVGRVMGADDRSYADLVQARSAELGIACELHPPT
                     ADPHALAALYGRARVVAVPSRFETLSMVVFEALACGRPVVMTDQIGAVEWLGAELPEL
                     VVPAEDVAALAAALRSHLLDADHAASVGERGRRAVASITADERVVADRLRVYQRAMGA
                     "
     gene            10439..11659
                     /locus_tag="VLR27_00220"
     CDS             10439..11659
                     /locus_tag="VLR27_00220"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HSP01892.1"
                     /translation="MPRTLQHRIGSAGRRLSLPLLRMLDVVAQRVVPVRHDRICYSSM
                     PDYTDNAYWVFRYLLDTREGLEHVWLLRDPAVADRIRADFAGAEARGHRLVLVPWTMR
                     SYLQFLRARVNFHTHGVYGFSRPGGRRISVCLWHGMPVKAIRRLHQGDHDLFPVHGTV
                     HLATSSFFRYIIAGVFDAHPSDVLVSGLPRTDVLKGFVAPGHDRQAIAEALGLDPDRR
                     WVLWMPTHRSEPARGRQPARTFLEATDRSLIDALDGACERLGAQVIVKLHPFDLLNDD
                     PVELEQPNLSLLTSAQWQATGIQLYDLVAASDALLTDVSSVLIDYLHTGNPVGVLGYD
                     PAAYTRDTLFDPELLLRCRAVHALATPDDVERFVAGMWESGGEPSDTADLTVVFNEDH
                     QIASTAHVVDAVGL"
     gene            11689..12138
                     /locus_tag="VLR27_00225"
     CDS             11689..12138
                     /locus_tag="VLR27_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HSP01893.1"
                     /translation="MRKIITFGTYDIFHVGHLRLLERAKALGDHLTVGLSTDELNERK
                     KGRLPVYPFDERLEILTSLRFVDAVFPEESLEAKRDYIVEHGADVLVMGDDWAGRFDE
                     LADICHVQYLPRTPAVSTTAVIEKIRRPLVSTGSAVGSAPAIESIRT"
     gene            12147..13067
                     /locus_tag="VLR27_00230"
     CDS             12147..13067
                     /locus_tag="VLR27_00230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSP01894.1"
                     /translation="MSSPADPVTVVVPNWNGEQLLPSVLDAVASQTRPPAAVVVVDNG
                     STDGSADVAADRPEVEVVRLPTNGGFGAAANAGLERCRTPNLVVLNSDARPEPDWLEA
                     LAGDGPCGDDIWAWGSVLVSAATGDVESAGDFVRWTGSSGKFLRGCPVGELPDEPYEV
                     LAPPGAAPLLRAEVVRDLGGWFEPYFLYYEDLDLAMRARLAGYRAHMVPSARVTHDLA
                     GSSGGRPPWDHIARSSTWCTIRTNPALSLQGFARLHAREYRIARRHGGTAGWARGKAR
                     ALPAIPRRVAERRRIMRAATDRAWVPGPPR"
     gene            13064..13732
                     /locus_tag="VLR27_00235"
     CDS             13064..13732
                     /locus_tag="VLR27_00235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HSP01895.1"
                     /translation="MSAIRRARRVLRRALARRDPHLTTRALNGPRTTRARLWLRTRRN
                     GSLLQAPFRITGAGAPHALGLIGVGSGTRIGHLAWFSLVTRDARIEIGADCMLSASLS
                     ISVVDRVEIGAGTAIGERVLITDHGHDHMTYLEPAIEGEEPPHFGYGTTEGHPVRIGS
                     GVHIGTNVYIGPGVTVGDGAVIGVNSVVTRSVPPYTVVAGIPAREIRSLRPTPTGGPA
                     CSQS"
     gene            13717..14397
                     /locus_tag="VLR27_00240"
     CDS             13717..14397
                     /locus_tag="VLR27_00240"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP01896.1"
                     /translation="MLAELKAELKYDLGHGVPRRFLNRTLEEFARSVPAGARTLEIGC
                     GYYDHRPHFAERLLRMDLYTEHEPDLNGDALAIPIADDAIDASICISVLEHVHDPYQA
                     VREWHRIMAPGGRVLAWIPFFFGVHGYPGDVSRFTEEGARHLFERAGFEIESTDTDTY
                     SGLFLNLGDAVHFTVPRTHHRPSVRALNRSLALACRAGLPLDRWLRLKRLYAGTTVLA
                     VKPGASSR"
     gene            14394..15083
                     /locus_tag="VLR27_00245"
     CDS             14394..15083
                     /locus_tag="VLR27_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01897.1"
                     /translation="MSWSSSLRATARRTARWRLARRRTLRWWVVYGPVRSGTTYMMEV
                     IAANARVRVSDWGLRHGLGLPPDLPHIRFDRARARADFSRNVLANAPAGGGRTLDLVC
                     KSAQMRPDELQALTEMWGPPERVIFCLRAPSGYLASAVRKFPDVDVQAFRDEYLADIE
                     THEAVGGDLFVYRPDLDTEDYRRFLAPLDIPDDPDLRFTYQGSTADDLVTPEMVDAFE
                     RVTGLVRARSR"
     gene            15080..15649
                     /locus_tag="VLR27_00250"
     CDS             15080..15649
                     /locus_tag="VLR27_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01898.1"
                     /translation="MTWRDRLRGRRGRFWSLQRLARLDADQLEALVGLARVVGREHVA
                     IRHESAGISERALISPLASLRFGDRLEIGPKATIGPYCAVWGGFEATTRIGAGALLAP
                     GAVVVAGNHRIDGEGWVRDLGFDEHDASVGEGAWIGAHATIVGCHVGAGAVVAANAVV
                     VDDVPADAVVAGAPARVVRMRRAGEGVPT"
     gene            15646..16326
                     /locus_tag="VLR27_00255"
     CDS             15646..16326
                     /locus_tag="VLR27_00255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP01899.1"
                     /translation="MTGTIGAPGSKADVYEAFGNPALLRAVVDHLAPGAAVLDVGCAS
                     GGLLARLATHAGRREGIEVDPVAAAAAADVADRVHVGSVDQMAPEGPPFDVVVLGDVL
                     EHVPDPAATLDRVLAWLSPEGRLVISLPNVAHWSVRLSLVAGRWEYRDSGILDDTHLR
                     FFTWASGRELVEGAGLRIVDRRPVISGLGAHLGRRLPATVDRAWRRIGVRRPNLFAYQ
                     QIIVAERG"
     gene            16328..17332
                     /locus_tag="VLR27_00260"
     CDS             16328..17332
                     /locus_tag="VLR27_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP01900.1"
                     /translation="MVGEPAVRLRGLTKVYEPSPRWMQLMTRSASKTSKVALDGVDLD
                     VHPGTICAVVGPNGAGKSTMFRLLMGLTTPTAGSAHVLGFDCAKRSESRRTPIGFMPA
                     DERSLFLRLSCKENLAFQAALRGYGGSRLDVRVAEVLDMVGLSESRDTAAFALSSGMK
                     ARLQLARAMVHEPAVLILDEPTSTVDPVAAGDLLELIQRLTSEQSLAVMLSSHRLEEI
                     DALDEQVVLLDDGNVVFQGDLRALRRIAALTEVVLELSGEGAAQDVAAALELVPEVAS
                     VARDGRVVELTANLTLGAVLASIGSRTADVVHVDERSSSTRSLLREVYANSRATRSAS
                     "
     gene            17329..18159
                     /locus_tag="VLR27_00265"
     CDS             17329..18159
                     /locus_tag="VLR27_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP01901.1"
                     /translation="MTAVVEHAQLLGRSARAFYLVGKSDALAYPLAFMLAIFSRLTNV
                     FLYYFVARIVTTDESVVAGGYFAFSVVGTLVLWVLGAGLELFGSFVQRTIDQGHLELY
                     LVQPVAWPLLPFLWFQWLVVERVVAGIATVVVAYALGVRFATDRLLLAVVVLALGVAA
                     THAIGVIAASVRMLSKRADPVLLAYTVASTVFSGLFVPVQVLPGILQSLSWLIPHTYV
                     VDALRQLLLDGGEASSRVSVGNAVLALGSFTVVAYAVGLVLFSKSLHYARRRGILGSY
                     "
     gene            18159..19085
                     /locus_tag="VLR27_00270"
     CDS             18159..19085
                     /locus_tag="VLR27_00270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSP01902.1"
                     /translation="MGAGDVVLPVVVVHYRAPEWCRSAVRAIRASTVAVDVVVVDNSG
                     ELGEAGVPGAAVVAPETNTGYAGGANAGIRHLLGNHPEATHIVVCSHDFHPEPGCFEL
                     LLGSASDAGLGIVAPRLTGPKPSIGNWFDGRRSTNIEPRPAATAVVDSDWVSGTCMAV
                     RVDVLVTVGGLDEGFGSYMEDVDLCLRARRAGWRVGTVVAAAGHGLGSVSDERFRLTA
                     VNVALLAAKREGARAGWALTARYAVRCLRSVLVAVLPSTRGLARRRASLRHARSRGGA
                     VLDLVRSRRIGTYARDPHHFEPRFAVHDRSHA"
     gene            19082..19750
                     /locus_tag="VLR27_00275"
     CDS             19082..19750
                     /locus_tag="VLR27_00275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HSP01903.1"
                     /translation="MSRALSLGFEVRRLVLVARLRVLAALKRADLDVVIDRGVRIEGR
                     VLVRVHGGTRTRVRIGRGTRIGDSVRLVLDGGELVVGERVLLRTGVVLHVRGRLTLED
                     ECLLSYYSVVHCDEEVHVGGRVGLGEHTTISDSTHVTPPPGDWWYHHIATAPVRLGAR
                     TWGGAKVTITRGVDIGADAVLAAGSVVTSDVPAGMLSGGMPARVLGPSPLSPASPDRT
                     PSGG"
     gene            complement(19754..21769)
                     /locus_tag="VLR27_00280"
     CDS             complement(19754..21769)
                     /locus_tag="VLR27_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01904.1"
                     /translation="MSATRSVAGFAGLALVSGAWWLVGTTLLRRWVPPLRSPLRQVSA
                     GVLALVGLLLAAAALGTVGWFRPWPLVVVSAIAGAAAIATMPRRQPVARDDEVAVSRR
                     DRWLVAGSVGLVAVRWGAELLQTMARGFSHADELHYHLTHSALFVQSGRTWPIRFTSV
                     GDGAAYHPAHAELLHGIGLSVLGSDFASIFVNLAFAAMAVAAGWAIGGRAGLPAAGAL
                     IVAAVLSLPLVVAESGSTLNDTMAIALLLAAAALLLAAVDRAGDLRLVAVAGAAAGAA
                     VGTKLTVVVAVVALTTLVAAGPVGTRLRAAGTYVGAALVAGGYWYVRNLAATGNPVPA
                     LSFGPLPGPELELQRAVEFPVTDYLTDLDIWRDHFVPGLAGFFGPLWPLLVGAVAVAA
                     VLALTSGVRRTAPQWALLAVCGLLSLVGYAVTPTSAAGGPGNPVLFQFNLRYAVPGAL
                     LCALAVLGHPGLRRRPHLATGLTAAAFVAANLHGVHPRRAVLAAAATVVVAVVVWALA
                     RRPLPRPRAALAGVALVLLALPVAVVAHDRYLDRRWSADLPRWAAFEAGDAATGLAVG
                     VTGYPQSYPFFGTGLDNRVVTLGDDRLGDELVPFETCGDWWQAVTDAELDVVVVLAER
                     GLHGTELGRWLVDEPARWLGAAGAPNLLQARDAAVFDVRGVQPACPA"
     gene            complement(21772..22584)
                     /locus_tag="VLR27_00285"
     CDS             complement(21772..22584)
                     /locus_tag="VLR27_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSP01905.1"
                     /translation="MQHAASSVAIAIPAYNEAEGIASFLHEIDEALHGHVDDVTFVVA
                     DDCSTDDTVEVLEALRPRLRADLVVESTGTNCGHGPAALAAYRRALDTGADHVIGVDG
                     DGQFLGTDIRRVLVLLEDGGDGVCGVRRFRYDPWFRMSMTRLLRVYIHRVFGVPTRDA
                     NCPLRGYRAPLLDELLRWIPDGSLIPNVHLTILAARRGATLVEVDVNHRVRRGESNTG
                     TMFASAGTIGVLKRLASFSVKALAESWRFRHDLNSGRRPMLDARHESHLRAA"
     gene            complement(22594..23727)
                     /locus_tag="VLR27_00290"
     CDS             complement(22594..23727)
                     /locus_tag="VLR27_00290"
                     /inference="COORDINATES: protein motif:HMM:NF015247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSP01906.1"
                     /translation="MKVLVIGAGWSGAVVARELHDQGVEVHVVERGEVVGGHARVEVL
                     NGVVYEPNGAHIFHTSNQRVAEYVQRFGMTRPYEHRVLTEVFLRDDDDTPRLLSWPPQ
                     IEELRALPDWPAIERELAELPPHPAGDDFESWVVSLMGRRLYELFIEGYTRKQWGCEP
                     SELSSRFAPKRVDLRDDGYTRLFRDTWEFFPADGANGIIEAILQPVPVTCGIEVGVDD
                     VDDLFGDVDDVVVTAPLDDFLGRPGELAWRGIRMVSRYQPSETEDGTVTPAYVVNRPS
                     TRVPHTRTVETKHASGQRVHGTVVSEEHPGAPARHYPVPTVDGRHEAQNAALQQEVRE
                     QLAKYRVHFCGRLSTYTYIDQDQAIDRAFACADGVLGGRILST"
     gene            complement(23724..24662)
                     /locus_tag="VLR27_00295"
     CDS             complement(23724..24662)
                     /locus_tag="VLR27_00295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP01907.1"
                     /translation="MGEAGADWGCPVCGSRHGTPAYRRQGDHIVDLRADELAPAATAF
                     GATVGEVVRCRACGHGSLDRPPSTDDLSAAYAHVEDASSLEEADGQTTTARRDLLEVR
                     DHLGAAPGRLLDIGCWTGSLLDAAADLGWTVSGVEPSTWAAARAVERGHDVTVGTLDD
                     VTLDAGSVQVVTCCDVLEHLDDPAAAARRIAELLEPGGLLFATVPDAGSVLARVLGRR
                     WWSVLPMHVQYFTRASLVALLTGAGFEVAAVHTHPKVFTYRYYADRLGEFVPAVGRPL
                     AGIVGRSPVAERAFGPDLRDRIAVVARRPRPAGGLR"
     gene            24783..26315
                     /locus_tag="VLR27_00300"
     CDS             24783..26315
                     /locus_tag="VLR27_00300"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HSP01908.1"
                     /translation="MLPSSEQRLRAYASVLVRHRLAVIGTTVAVLLVGLVPALLADET
                     YASTASVRVRPETAGSPFSDTDEPNAQNRSRELVTDVEVIQSDRLRDLVGDRVDGDFG
                     TAEATLVGFSEVIEITVSAGDPDLAAAAANAYADVYVSERQRRAVETLVDQSEELRRR
                     SDEAAAEMAAIDAQLGAPGTDPVTAENLRVRRASLADQVLDFSARADELEVEAALREG
                     GTEIVNPAKPEPDPVSPDPVRSALMAGVLGLLAGIGVAVVLDVVQDRISSPDDLVVLD
                     PSLPVLTSVPHLARAPQRPVDLDPRGREAFRYLRASLHFRSLDRPIQSLLVASAVAEE
                     GKTTTAVNLAVACAEAGARVVLVDADLRRPSVHQCFDLELEPGLTDVLAGRVTFADAI
                     TYVEPNLAVLPAGASAAGSHELLERPAFAQLLQAAAKKSDLVVVDAPPVLQAADALVA
                     ARAIDAALVVARVGQVRRREVRALLRRLRDARIPLIGFVANGGTAEVSYGEYAPLAGD
                     RA"
     gene            complement(26317..27057)
                     /locus_tag="VLR27_00305"
     CDS             complement(26317..27057)
                     /locus_tag="VLR27_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01909.1"
                     /translation="MILSHRHRFVFVKTGKTAGTSLKIALAKLCGPEDVVPPTMDPSH
                     DLAPRNHLGRANPLPELRIARRAGIVLDAAGTRQMLLRRKKFGGHTPAWIIRARAPRE
                     WREYTTFCVERNPWDKVLSGLSWVRTQYGQDWDVDTYLDFVEDRIAKGYHGTGVSPYN
                     HRSYTDPSNGEVIVDRILRYEHLDEQLPVLLDELGLPVPELPRVYGDRRRSGVPTALT
                     EGQIARVARLFAPEIELHGYRPPEPVGG"
     gene            complement(27070..28815)
                     /locus_tag="VLR27_00310"
     CDS             complement(27070..28815)
                     /locus_tag="VLR27_00310"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSP01910.1"
                     /translation="MRSLLPHRLRLLTMVALAALLAVLAVPSASAEAQPSGDPADGAT
                     EQPLPATALTFRVDGSFSVQDVWPDGSLELPFPTVGWAIAATESLRAAGQPVRIVVAP
                     GTYRERVKITGTGADAPLVLESEEPGGAVITGADVEDRWTPVAGTPLARAPWTANWGL
                     APIPGSWGDAVAPSDGVRRREAMFVDGEPLVQVLRFEDLVPGSFLVDETGDAIYAHPP
                     AGASPDLSGNLVEVATRARVLDIGNGAANVTVRGFTFQAGAAPFTKFMANVSESTDVV
                     VEQNTFRHSSWGGLFVCCTEGVTVRDNVSEANGGNGIDTYRAHDVVVQGNVATGNNVR
                     GARNGYLGWSVAGSKNMLLHGAVFRDNVWDGNWARGLWFDTDVVDVLVDGDRSCGNAR
                     DGVFLEALAGPITIRNSTFCGNARAGVIVSSAGGVTLEGSTLADNPYGQLVFSGERLR
                     TWTNHVTGTPELVQDFTDWTLRDNVLQSDATNSPLVFSPVLTQAEWRAKLSAGEVRAS
                     GTIWDHPSIAEAIIIYGKAFSIAEWNSLTGDGTATADPAPEPVVEEPTDPSTSRGNSG
                     KGDKSGPPQGKGPRS"
     gene            complement(28898..29683)
                     /locus_tag="VLR27_00315"
     CDS             complement(28898..29683)
                     /locus_tag="VLR27_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HSP01911.1"
                     /translation="MSKEPMLRRALAVAIAVVASAGIVSAGVVTNPVWADAALERAEV
                     VASPDAQRVLSAAAGEPAVPLAPPPAAATEQAPEPPPPAPAPAPAPDPTQAPAAAPSP
                     TAPPPTFAPAPAPAPVATTAPAPPPPQPAAARTGSRDAECESSMLRWMNETRAGAGRH
                     ALAWDDAILHVAVDWSHEMADRSELAHNPRYGDQVFAARAEAMTAAENVGWGNGSARA
                     VYDEFLRSPSHRDKILSGALTHAAVGCVRDGAGELWVTVNFWG"
     gene            complement(29792..30211)
                     /locus_tag="VLR27_00320"
     CDS             complement(29792..30211)
                     /locus_tag="VLR27_00320"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HSP01912.1"
                     /translation="MTDTTPAPEILVVCTHNAGRSVAARVLLDHHGQGRVTVRSAGSA
                     PGDDINPAVAQILTERGLDVSKEFPKPLTDEAARAADVIVTMGCGDACPIFPGKRYLD
                     WDLTDPAGKPVEEVRPIVDDIDRRARALLAELLPPAG"
     gene            complement(30208..31293)
                     /gene="arsB"
                     /locus_tag="VLR27_00325"
     CDS             complement(30208..31293)
                     /gene="arsB"
                     /locus_tag="VLR27_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HSP01913.1"
                     /translation="MTVVTEPRPEDAPVLEKLSTLDRFLPVWIGAAMALGIALGKLFP
                     GLDETLDRIKIDTVSLPIAIGLLLMMYPVLAKVRYGNLGEVTADRRMLGLSLVLNWVI
                     GPALMFALAWLLLPDLPEYRTGLIIVGLARCIAMVLIWNDLACGNREAAAFLVAVNSV
                     FQIAAYSLLGYFYLELLPSWLGFDQEALDVSIWEIAKSVLIFLGIPLLAGFLTRLIGE
                     RRKGTEWYEGTFLPRIGPVALYGLLFTIVVLFALQGDAITSEPFDVARIALPLLIYFA
                     IMWGSSFAIGLRMGLPYDRNTTVAFTAAGNNFELAIAVAISVFGVTSGQALAGVVGPL
                     IEVPVLVGLVYVALWARRRYYPTGDPS"
     gene            complement(31290..31613)
                     /locus_tag="VLR27_00330"
     CDS             complement(31290..31613)
                     /locus_tag="VLR27_00330"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSP01914.1"
                     /translation="MTVDVEVAAPPTAVLKALAEPNRWRIVELLSCEELCVCHLAEDL
                     DMAQPLVSHHLKVLRDAGLVESEKHRQWTYYRLRRDQLEALGGQLRSITSCCPAPGER
                     QRACT"
     gene            31695..32885
                     /locus_tag="VLR27_00335"
     CDS             31695..32885
                     /locus_tag="VLR27_00335"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HSP01915.1"
                     /translation="MSRPRVVILSGARTPIGKLSGSLSSFSAMELGGFAVSEALTRGG
                     LRGEQVDYVYMGHVLQAGAGQITARQAAVNGGIPMDVPATTVNKVCLSGMDAIYQADR
                     RIRAGDAEIVMAGGMESMTNAPHLLPGARAGYRIGDGTIVDSMMYDGLYCAFDSCAMG
                     EGTERYSQAHGMEREHMDAMAAESHRRADAAQQAGLFEHEIVPVTVPQRKGDALTVTH
                     DEGVRGETTIESLAGLRPAFDRNGHITAGNASQISDGASATVITTLEVAERLGVSPLA
                     EILGYGQVAGPDASLLHQPSRAIKDALKRTGLSLADLDLFEINEAFAAVGVASADDLG
                     ISREVVNVNGGAIAVGHPLGMSGNRIALHLALELARRGGGTGAAALCGGGGQGDAVIV
                     RTMS"
     gene            32896..33354
                     /locus_tag="VLR27_00340"
     CDS             32896..33354
                     /locus_tag="VLR27_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSP01916.1"
                     /translation="MSAPSADPGRTYQAHAETEGTGVGQIRTKRAVVRFDSSPSQGDE
                     LPGPADLLTSAFAACIIKNVERMGEMLPFAYDVASVEVTAERQDRPPKIVRIRYVVTV
                     VTDEPPHRVNLLHRNIQRHGTIFNTLAAVCEVAGTIEARPSPRVGDAHPG"
     gene            complement(33378..>34089)
                     /gene="arsM"
                     /locus_tag="VLR27_00345"
     CDS             complement(33378..>34089)
                     /gene="arsM"
                     /locus_tag="VLR27_00345"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HSP01917.1"
                     /translation="DAIHTQVRAHFAQAATSPDPSCSSPDDEPWGTKHYDHLDEDLPA
                     EAVLASLGCGNPTAVADLQPGDTVLDLGSGGGIDVLLSARRVGPTGYVYGLDMTPEML
                     DLARRNAAEARATNVEFLEGTIEQVPLPDSSVDVIISNCVVNLAADKHAVFDEMFRVL
                     RPGGHIGVSDVVTDDDLSDDDRAATGSYVGCIAGALRITEYEAGLTAAGFTDVRVTVT
                     NDVDDGVHSAIIKATKPD"
BASE COUNT         4672 a        12273 c        12580 g         4564 t
ORIGIN      
        1 gaaggtcttc accgagatcc agcgcctcgg ccaggtcagc gacgacgaga tggccaaggt
       61 gttcaacatg ggcatcggca tgatcgccgt cgtccccgcc gaagaggtct acgccgccct
      121 cgacgtgctg cgcaccgccg gacaccgcgc cgtgcagatc ggcaccgtcg aacccggaag
      181 cggcctcgtc cggtacgcgt agttcgcccc cggtccctgt acggtcgccc gccgtgcgca
      241 cggtggtccg gacggtgtcg tgggtgctgc tcttcgcggc gctgggagca gcgggcgcgt
      301 cgtggttcac ccccacgccg aagctggagg cagaccacgc ctctgacctc gccctcggcg
      361 ccctgcgcga ggccgaggtg gacgtcgagc gcgtgaagcc accggtgcgg atggtgcacg
      421 agacccgaga cgacgtcctc gtcgacgcct ggcgcgtcgt ggtcgacgtg caggcccaag
      481 gtgcggtcga ggaggtcgag ctccgcgtcc aggagtccgc cggccagctc gtcttcgtcg
      541 acgacctcat cggcgccgac ggtacggagc ggctgctgtc cgaggagcag ttcgaggtga
      601 tgagccagca ccgcgacgac accctggccg accgctgggt gctccggaac gcgatggccg
      661 cggtcgccgc catcggcatc gccgccaccg cctacatcct cgccacccga tccgatccgc
      721 tctggagcgc ccgttgaccc cgtcccgcac cgtccccgcc gaggcccacg ccgcgctggt
      781 ggcccacctg aaggcccacg ccatccgcga gggcgacttc gtgctcaagt ccggcaagcc
      841 gacgacctgg ttctgcgacg ccaagcagac gacgtgccgg cccgacggca tgctgctggt
      901 cgccgacgcc gcactcgccg tcctgcctga cgacgtcacc gcgatcggcg ggctgacgat
      961 gggtgccgac gccgtggcgt tcggggtcgc cgcgatcgcc gccacacggg gccgcgagct
     1021 gcgggccttc agcgtgcgca aggaggtcaa ggaccacggc gggggtgggc gcgtcgccgg
     1081 cgcgctcgaa ccgggggacc gggtcgtgat cgtcgaggac gcggtgaccc ggggcgtctc
     1141 gatgctggag gccgccgacg tgatcgcggc ggaggtcggt gccgaagccg tgctgctgat
     1201 gccggtggtg gaccggggcg ggacggtggc ggccatggcc gccgagcggg ggctgcccct
     1261 gcaggcgctg gtgaccgcgc cggacctggg cttcgactac gaggggccgc tgtagagggc
     1321 tggcagccgg gactacgagg ggccgctgta gaggctggca gccgggaccc cggcgggctc
     1381 gagccggtcg gtcaggtgcc actggagctc gaccaggccg tggagggcca ggatcgccgc
     1441 accgatgatg ccggtggtgg cgtcagcccc catggccagg ccgatgacga cggtgggatg
     1501 ggacagggcg aacccgatgc gggcccgacg tcgggcgtcg gcatcgtcgg ggtgggcact
     1561 gctcggtcgg tggctggcgg tcgcgaacat cgatgcgtgc gtacccggcc gccgcgacca
     1621 ccagaacagg aatccctgac ggggtggctg ccagttccgc tcaggggcga ccggtgcgca
     1681 ggcgggcgac ccgggcgacg gactcgatgc ggtcctcggc gtggcggtcg gcggcctcgc
     1741 ccggcgggat gctctgggtc cgggcccgct ccagcacggc caacaccgtc tcgtggatgc
     1801 cggcgatccg ggcccacgcc cgggtctggt cgtaaccggc cggctcctcg gcgatgttga
     1861 tcacgccgcc ggcgttggcg acgtagtccg ggacgtagag cacgtcgtgg tccacgaggc
     1921 gctggtcgtc ctcgggagtg gcgagctggt tgttggccga gccgacgacg acctcgcagc
     1981 gcagctcggg gatggtgcgc tcgtcgagga ccgcaccgag ggcgcacggg gcgaagacgt
     2041 cgcagtcgac ggcgtgggcc tggtcgggct cgacggtgcg cagccgctgg cgggcctcgg
     2101 ggtggtcgtc gtggacggcc cggacggcgt cgtcgcggat gtcggcgacg gtgacggtgg
     2161 cgccctcgtc caccaggtgg tcgacgagcg cccggccgac cttgccgacg ccggacacgg
     2221 tgacgtggcg tccggacagg tcgggtgagc cccaccggac ctcggccgcg gcgcgcagcg
     2281 cccacaggac cccccacgcc gtggccgggg acgggtcgcc cgacccgccc agcgacgggc
     2341 tgacgccggt gacgtgcggg gtctcgcgcc ggatgaggtc catgtcggcc tgggtggtgc
     2401 cgacatcctc ggcggtcagg tagcggccgg ccaggccgtg cacgaagcgg ccgtaggcgc
     2461 ggatgagggc ctcggaccgg atcgtggccg ggtcccccca gatcaccgcc ttgccaccgc
     2521 cctggtcgag gccggcggcg gcgtgcttgt aggtcatgcc ccgagcgagg cggcaggcgt
     2581 cggtgagcgc ctcggtctcg gtgtcgtagg gccagaaccg ggtgccaccg agggcggggc
     2641 cgagcaccgt ggaatggatg gccacgatcg ctcgcaggcc cgaggggcgg tggtggcaga
     2701 aggcgacctg ctcgaagtcg ccggcgtcgg gcaggccgtc caccgcgtcc atcgtctcga
     2761 acacggtgct ggctgcggtc atgtccggac cgtaccgctg accgcaagcg gacagaacgg
     2821 gactagtccg tcaccccctc aggaaatgac ccgagtgggc tatcgccgtt ctgcggccca
     2881 ctccgtacct ttgggaagga accggacaca ccagtggacc ggagaacgag gaagtcacag
     2941 cacccatggc agatcaagaa gatctcgaca cccccgaagc gctcctcacc ccgtccgagg
     3001 tcgccgcgat gttccgcgtc aaccccaaga cggtcacccg ctgggcgcgc gccggcaagc
     3061 tcaacgccat ccggaccctc ggcggccacc gccgcttcaa ggcgtccgag atccgtgcgt
     3121 gcctcgagga gatgtcccag tcggaggtgt gagcgtcggc tgcgcccgac cgcagcacac
     3181 gccgtagtct cggccgccat ggccactcca accgctgcct tctccctcca cctgcgggtc
     3241 cgcctcgaga acacccccgg gtcgctcggg cgactggccg tggccatcgg tgacgcgggc
     3301 gggaacatca cccagctcga gggcttcgac gtgcgcgggg agtacctcga cgaggacatc
     3361 gtcgtgaact gcatctccga ggcgcaccag gaggaggtca aggcggcgat cagcggcctc
     3421 gacggcgtcg agatcacgac ctgcaaggac cgcaccttcg agatgcacga gggcggcaag
     3481 atcgaggtcc tcgcccggat gcccatccgg gacgccgacg acctctcgat ggcgtacacc
     3541 cccggcgtcg cccgggtctg catggccatc cacgccgacc gcgagcgcag ccgcgacctc
     3601 accatccgca agaacaccgt cgccatcgtc tccgacggca ccgccgtgct cggcctcggc
     3661 gacatcggcc cccacgccgc catgcccgtg atggagggca aggcgctgct gttcaagaac
     3721 ttcgccggcg tcgacgcctt cccggtgtgc atcgacacca ccgacaccga ggagatcatc
     3781 gccaccgtcg agcgcctggc gccgaccttc ggcggcatca acctcgagga catcgccgca
     3841 cccaagtgct tcgagatcga ggagcgcctc aaggccagcc tcgacatccc ggtgttccac
     3901 gacgaccagc acggcaccgc cgtcgtggtg ctcgccgccc tgcagaacgc cctcaagctg
     3961 accgacaaga agatgagcga cctgcgcgtg gtcatcgccg gcgtcggtgc ggccggggtg
     4021 gcctgcggca agatcctgct cgaggccggg gtcgctgaga tcatcggcgc cgaccgcaag
     4081 ggcgccatct gggagggtcg cgacgacctc aaccccgcca agcagtggtt cgccgagcac
     4141 accaaccgcg accgccgcaa gggtgcggtg ggcgagctgc tcgccggcgg cgacgtcttc
     4201 atcggcgtgg ccggcccggg gatcatcgac cccaccgacc tcgaccggat gaacaccgac
     4261 ccgttcgtgt tcgcgctggc caaccccgac cccgagatcc gccccgagca ggccaagggc
     4321 cgggccaaga tcatcgccac gggtcgcagc gacttcccga accagatcaa caacgtgctg
     4381 gccttcccgg gcatcttccg gggtgcgctc gacgccaagg ccaccgacat caccgagggc
     4441 atgaagctgg cggcggccga ggccatcgct gacgccatcc cgatgtcgga gctgcgtccc
     4501 gacttcatca tcccgtcggt gttcgacgcg acggtggccc cgcgcgtcgc cgccgcggtc
     4561 gccgaggccg ccgtggccga aggcgtctgc cgggactgag cgggacctgt gatcccatga
     4621 ccgaccgcgg gcccgacgag ctcgacgtgg acctccggtg gtccgagtcg gacgagccgg
     4681 acgccgggcc ggtcgagctg acctggaacc gtccggccac gggcgaggag cgaagcgccg
     4741 ccacgggcac cgtccgtcga ggggtgcgcc tccgccccgg tgcgagccgg accacgagcg
     4801 cgctgcgtgc cgaggtcgac gagctgcgcg ccgaggtcga gcgcctccgg gccgagctcg
     4861 atgcgctccg ggccgggctg gagcagtccg gctgagcctc ccagtgcgtc agtcggcgct
     4921 gccggcgacg gccagcgcgg cggtggcggg gttcgggtgc acgggcggtg cgtccagcag
     4981 cgatggtgcc ggacgctcgg cgatggcgat gggtgggaag acgggcgcgg gcgacggcgc
     5041 gtggcggacc aggcggtggg gcacctcgta gagctgcgtg gtccggccga gggacatctt
     5101 gcgcagggtg cggagcagga tccaggcgtc gaaggtcgcg gtgcggtgcc agatgtagag
     5161 ccggtcgtac tcggtgcgct cgccgatgag ctggtggcag tgcgggctga tctgccacag
     5221 gccggtgatg ccggggcgca ccgacacccg ctcggacgcg aagggctccg gaagccgggc
     5281 gtggaggttc ggcatctccg ggcggggccc gacgaaggcc atctcgcccc gcgccacgtg
     5341 cagcagctgg gggagctcgt cgaggtgcag tcgccggacc gtgcgcatca ccgccgggat
     5401 ccgcgacgcc tggagggagt acttgtcggc gtaccgggcc gtgtcgggcg gcaggctccg
     5461 caccttcacg aaccgcaacg tctgcccgcc gaggccgacc cggtcctgga cgaagaacgg
     5521 ccaggcccgg tagcaggcga acgacacggc ggcggtgagc gcgatcagcg ggactgcgag
     5581 caggagcagg acggcagcca ggctgcgctc caggaacctg cgtgcgaggt catcggcacc
     5641 catgtgcgat ctgcccttcc ccgacccccg tcgtggctgg tgactggacc cttcgtctcc
     5701 ctgcggggag attcctgccg gtgcgtccga aaatgttcgc cacccgttca ccgggtggcc
     5761 acggtcaggc ggcccggtcc ggccggacga gcagggcggg gtgcttgcgg gcgaagcggg
     5821 ccatcccgcg caggtgccag acgttgcgcc gggacaggaa tcggcggtcg atgacggcct
     5881 ggtactcgtg caccgccccg gcctcgggca ggacccagcg ctcgtggccg gcggcgcgga
     5941 ggcgggcgca gaggtcgatg tcctcgacgt acatggggta gcgctcgtcc atgccgccga
     6001 cctcgtcgaa cgcgcggcgg cggaccaccc agaacgcgcc gagcagccag tcggccggcc
     6061 acgggtcggc ctggtcaccc aagcggtagt gccggcgctg gaggtcgaag ggcgggaaca
     6121 ggagccgcag cggcgtgcgg cgcacgaggg tggcacccac ggtggggaag gaccgtccgg
     6181 tcggttgccg gcgcccgtcg ggccaccgca gctgcggtgc gacgatgccc gcccgggggt
     6241 ggtcgtcgag gaacgcgcac aggcgggaga tggcgccggg gtccggcacg cagtccgggt
     6301 tgaccacggc gacgtagggc gcctcggtgc gggcgaccgc caggttcacg ttctccgcga
     6361 aggacctggg ctgggggttg gtgacgacca cgccgttggg cacgtccggc aacggcgtcg
     6421 ggggcaggtt gtcgacgatg accagcgagc gcacgtcacg gctcaccgag gtgatgcacg
     6481 aggtcaggtc gtcgttggtc ccgtgggtga ccacgacgcc gtcaacgggg tgcatcgacg
     6541 atgccctccc gggatccggc gagctcggcg acgacgtcga gcatccccca gcgcaggtcg
     6601 tcggcgaagc gccgctcgta ctcgcgccgg ttgcgctcgg agcgagcctc gcctggcgac
     6661 cagcgcgacg cggcctcgac gagcccgtcg gccaggctcc gtcgcaggtc gtcggcggac
     6721 ccgccgggct ggaccaccac gcggtcgcag gcctcggtga cgacgtcgag gttgccgcgc
     6781 cattcggtca ccacgatggg gagcgcgaac atgagcgcct cggcgagcac gaggccgaag
     6841 gactcgcacg ggaacaccga cgggaagacg aagcagtcgg cgccggcgaa ggcctcccac
     6901 gtcgcgtcac cgcgcagctc cgggtcccag tggacggtgg ccggctccgg caccgaggcg
     6961 atgtcggccc ggagggactc gaccgtgtag ggctcgaggc agtcgcccac caggtgcagg
     7021 tgcagcgcca cgtcggtgcg cgacgcagcg tcgccgaggg ccccgagcag cgccggggtg
     7081 cctcgctggg gcccgaggtg gccgacgtag agcaggtgca gggcacctcg gtcggcgccg
     7141 tcgaccgggc gccgggtgcc ggcccggtcc gggatcgggt ggggcaccac cgaggtctcg
     7201 gccacgccca ggaacgtcgg atcctcccgg ttgaactgcg tcatgaccac ggcccggtcc
     7261 gcccgccggt aggcagtccg ggcgaggcgc atccaccacc gcatcgggcg ccagtgggcg
     7321 gcgtggccgg caccgtgcat gtggagcacc gtgcggcggg cggccaggcg cacgaacggc
     7381 aggatcgccg cgtcgcgcag cgacgactgc ggggccacgc cgccgaccgg gtagacggcc
     7441 acgtcggccc gaccgtgccg gcgctcccgg acgagggcga ccatcgtggc caggagcgtc
     7501 acgaccttgc cgagcgacac gtggcgcatc gagccgatgg actccgagaa gcgaggtgac
     7561 acgtggcgga cctcccaggt cccctcggca cggagcgcgt ccagcaggcg ctgcaccatg
     7621 acgtgctgcc cggacatggg tggcgggacc tgcccgatga ccagcacccg gggcttcggc
     7681 gtcgccgtca cgtcgccgct ccggctcgct ggtgcaggtc ggccccgcgc tcggtcgtgt
     7741 cggtcctcgg gatcgctcgc cagccctgcg ccgcgccggt caccaggaag agcacggtga
     7801 gcgccaggtg gggttcggcc aggccgtcgc tgctcacggc gccgacgacg ctgaacagga
     7861 acatcgcgag cagggcgcgc ccggcgtcga cgcggagcag cccgctggac accagcaggg
     7921 acccggcccg caccagcgcg gcgacgagca gcgcggcccc gaccaggccg agcccgacga
     7981 gggtgccgag cagcgagctg tgggcgttgc cggcgaacgc gaagtcgccg atgaacacgg
     8041 tgcgggtcgc gccgtagccc agcccgacca gccagtcggt gggcccgtcg agggcggcca
     8101 ggccgacgtc ccacaggtcg gtgcgcccgt tgagcgtggt cagccggctc acgtcgccgc
     8161 cccggaggat gtaggacgag atcgccccgc cgacgaacag cgcggcgacc accccgaggg
     8221 ccccggtggc gtaggtggcg aaccacgcgg ccccggagcc gctgcgcacc cacaggagca
     8281 ggagcgcaag gcctccgacc accacgccgg cgagcaccgt gcgcgtctgg ttctggtaca
     8341 gcgcgaccgc gaccgcgagg acggcgccgg cccggaacca cgccgggacc cgcaggcggc
     8401 gggcgtcggt ggcgagcagc acgagcagcg cggtgccgag catgaagccg gactcggtgg
     8461 ggtgggcgcc gatccaggtg aagcggtccc ggtttgccgc ggtgacgacg acgaacatcg
     8521 gcccgagcgc gaccccggcg gcgatgagcg cgagcgtggc gagcacgagc cagcggagca
     8581 ccaggccgag catccggtcg agcgcgtcgc gggagcgcca gccgatgccg gccgtgacca
     8641 cggcgagcag cagcagggcg acgtactcgg cgccgcgggc gagcgcgtag gcgggtgcgg
     8701 cggcccaggc ggccgaggcc agcacccaca ccgggaccag ggtgatcggc gcacccaccg
     8761 gctgccacgg cacgacggcg aggagcagga ggagcgccac ggcaccggct gcgccgtaga
     8821 tcaggagctc gagcccggtc tgcagggtca gcgtggagac ggccgcgctc gccgactgcc
     8881 ggccgatgtc gtgcatcggt agcaggccga cgacgaccca cgacgcagcg atcgcggaga
     8941 gcacgcggtc gtcgaggacg tcgcctgcgg gggtcggtgt cggggtcgag cggccgtgcc
     9001 ggtgccaccg caggagcacg acgtcgccga tgaccagggc accgcccacg atgatggcga
     9061 tcgcaccgcc gacccgggcc gccgtgccga tcgccagcac accgaggagc gcgcccgagg
     9121 cgacgaaggc gggtgggcgc gagcgcatca ccgcgtgggc ttcgccaccc cccggcgaac
     9181 ctcctctcgc cgatcccacg gcacgcgtcc ctggcaggcg ctcgcgccgc gagggctacc
     9241 gtgcccgccc catgcggatc gccctcgtga ccctgcagta ccccccgacg ggtgtgggcg
     9301 gcatcggcac gtacgtcgag acgctcgcct cggcgctcgc aggggccggc cacgaggtca
     9361 cggtggtgtg ctcggcacgg gaccaacgtc gctcctcggc ggtggagggc ggggtccggg
     9421 tggagcgctt ctcgaccctc ggacccgggt ggctgcggga ccgcctcgtc gctccccacc
     9481 aggccctgcg ggtccggatc ctccatgcgc tgtcgtcggc gtgggcgctg tggcgcgtcc
     9541 gctcgcggtt cgacgtcgtg gaggtgccgg agtggaaggc gcagggcctg ctgctccgct
     9601 tcgtccgccg cggcgccgtc gtggtccacc tgcacctcgg cttcgagctg caggcggcgt
     9661 ggaacggcgc gaccgcctcg cgggggtggc gcatctcgtt cgcgctggag cgctggacgg
     9721 cgaggctcgc ccgggtgcgc acggccacgt cggagcagac acgccggctg cccgacggct
     9781 ccacctggct cgcggacgag gaggtcgagg tcgtcgcgcc gccgctgcgc agcggaccct
     9841 ggcgcagctg ccccccggtg caggacaccg agccggtggt gctcttcgtc ggccggctgg
     9901 aacgacgcaa ggcaccggag gtgctggtcg aggccctcgg ctcgatcgct gcggagatcc
     9961 cggggtgccg ggtggtcttc gtcgggcggg tgatgggtgc cgacgaccgc tcctacgccg
    10021 acctcgtcca ggcgcggtca gccgaactgg gcatcgcctg cgagctccac ccgcccacgg
    10081 ccgacccaca tgcgctcgcc gcgctgtacg ggcgggcgcg ggtcgtggcc gtgccgagcc
    10141 ggttcgagac gctgtcgatg gtggtcttcg aggcgctggc ttgcggccgg ccggtggtga
    10201 tgaccgacca aatcggggcg gtcgagtggc tcggtgccga gctgcccgag ctggtggtcc
    10261 cggccgagga cgtcgcggcg ctcgccgccg ccctccggtc ccacctgctg gatgccgacc
    10321 acgccgcatc ggtgggggag cgcggacgtc gtgccgtcgc gtcgatcacg gcggacgagc
    10381 gggtcgtcgc cgaccgcctc cgggtgtacc agcgggcgat gggggcctga tcgtggcgat
    10441 gccccgcacc ctccagcacc ggatcggcag cgccggtcgc cgcctcagcc tcccgctcct
    10501 gcggatgctc gacgtcgtcg cccagcgggt cgtgcccgtc cgccacgacc gcatctgcta
    10561 ctcgagcatg ccggactaca ccgacaacgc gtactgggtg ttccggtacc tgctcgacac
    10621 gcgagagggg ctcgagcacg tgtggttgct gcgggacccc gccgtggctg atcgcatccg
    10681 cgccgacttc gccggcgccg aggcgcgcgg ccaccgactg gtgctcgtgc cgtggacgat
    10741 gcgctcgtac ctgcagttcc tgcgagcgcg ggtgaacttc cacacccacg gcgtgtacgg
    10801 gttctcccgg cccggcggtc gccggatctc ggtgtgcctg tggcacggca tgccggtgaa
    10861 ggccatccgc cgcctccacc agggggacca cgacctgttc ccggtgcacg gcacggtgca
    10921 cctggccacg tcgagcttct tccgctacat catcgccggc gtcttcgatg cgcacccctc
    10981 cgacgtcctg gtgagcggct tgccccgcac cgacgtcctg aagggcttcg tggcgccggg
    11041 ccacgaccgg caggccatcg ccgaggccct gggcctcgac cccgaccgcc gttgggtgct
    11101 gtggatgccc acgcaccgct ccgaaccggc gcggggccgt cagccggcgc gcaccttcct
    11161 cgaggccacc gatcgctcgc tgatcgatgc cctcgacggg gcctgcgagc gcctgggcgc
    11221 ccaggtcatc gtgaagctgc accccttcga cctgctcaac gacgacccgg tcgagctcga
    11281 gcagccgaac ctgtcgctgc tgacgtcggc gcagtggcag gccaccggca tccagctgta
    11341 cgacctggtc gcggcatccg acgccctcct gaccgacgtg tcctcggtgc tcatcgacta
    11401 cctccacacc gggaaccccg tgggcgtgct cggctacgac cccgcggcgt acacccgtga
    11461 cacgctcttc gaccccgagc tgctgctccg ctgccgtgcc gtccacgccc tcgccacgcc
    11521 cgacgacgtc gagcgcttcg tggcagggat gtgggaatcc ggtggcgaac cgtcagacac
    11581 cgccgatctg accgttgtct tcaacgaaga ccaccagatc gcgtcgacgg cccacgtcgt
    11641 cgacgccgtc gggctgtagt ccggcatgcc gacacgacgg ggtagacgat gcgaaagatc
    11701 atcacgttcg ggacctacga catcttccac gtgggtcacc tgcggctcct cgagcgggcc
    11761 aaggcgctcg gcgaccacct caccgtcggc ctgagcacgg acgagctgaa cgagcgcaag
    11821 aagggccgtt tgcccgtgta cccgttcgac gagcgcctcg agatcctcac cagcctccgg
    11881 ttcgtggacg ccgtcttccc cgaggagtcc ctggaggcca agcgcgacta catcgtcgag
    11941 cacggcgccg acgtcctggt catgggcgac gactgggcgg gccgcttcga cgagctcgcc
    12001 gacatctgcc acgtgcagta cctgccccgg acccctgcgg tgtcgaccac cgccgtgatc
    12061 gagaagatcc gccggccgct ggtgtcgacc gggtccgccg tcgggtccgc gccggccatc
    12121 gagtcgatcc ggacctgacc gtgcccgtga gctcgccggc cgatcccgtc acggtcgtcg
    12181 tgcccaactg gaacggggag cagctgctgc cgtcggtgct cgacgccgtc gcgagccaga
    12241 cccgaccgcc ggccgcggtc gtcgtcgtcg acaacggctc caccgacggc tccgctgacg
    12301 tcgccgccga tcgtcccgag gtcgaggtcg tccgcctgcc cacgaacggc gggttcggtg
    12361 ccgccgccaa cgccggactc gagcggtgcc gcacgcccaa cctggtggtg ctcaactccg
    12421 acgcccgtcc cgaacccgac tggctcgagg ccctcgcggg cgacggcccc tgcggggacg
    12481 acatctgggc atggggcagc gtgctggtct ccgcggccac cggcgacgtc gagtccgccg
    12541 gcgacttcgt ccggtggacg ggcagctcgg ggaagttcct gcgcggctgc ccggtcggcg
    12601 agctgcccga cgagccctac gaggtgctgg ccccgcccgg cgccgcgcct ctgctccggg
    12661 ccgaggtcgt ccgcgacctc ggcggctggt tcgagccgta cttcctctac tacgaggacc
    12721 tcgacctcgc catgcgggcc cgcctcgccg gctaccgggc ccacatggtc ccgtcggccc
    12781 gcgtgaccca cgacctcgcc ggctccagcg ggggccgacc gccgtgggac cacatcgccc
    12841 ggtccagcac ctggtgcacg atccgcacga acccggcgct gtcgctccag gggttcgccc
    12901 gcctccacgc acgcgagtac cgcatcgccc gacgccacgg cggaacggcc ggctgggccc
    12961 ggggcaaggc ccgggcgctg cctgccatcc cgcgacgggt ggcggagcgg cgccggatca
    13021 tgcgcgccgc caccgaccgc gcctgggtgc cggggccgcc gcgatgagcg ccatccgacg
    13081 cgcccgacgg gtcctgcgcc gcgcgctcgc tcggcgggat ccgcacctga cgacccgcgc
    13141 cctcaacggc ccgcggacca cccgcgcccg cctctggctc cgcacccgcc gcaacgggtc
    13201 gctgctgcag gccccgttcc gcatcacggg tgccggcgca ccgcacgcgc tcggtctcat
    13261 cggcgtcggc tccggcaccc ggatcggcca cctcgcctgg ttcagcctcg tgacccgcga
    13321 cgcccgcatc gagatcggtg ccgactgcat gctgagcgcc tcgctcagca tctcggtcgt
    13381 cgaccgcgtg gagatcggcg cgggcaccgc catcggtgag cgggtcctga tcaccgacca
    13441 cggccacgac cacatgacgt acctcgagcc cgccatcgag ggcgaggagc cgccgcactt
    13501 cggctacggc accaccgagg ggcaccccgt gcggatcgga tccggggtgc acatcggcac
    13561 caacgtctac atcgggcccg gcgtgaccgt gggcgacggc gccgtcatcg gcgtgaactc
    13621 cgtcgtgacc cggtcggtgc ccccgtacac cgtcgtcgcc ggcatccccg cccgggagat
    13681 ccgctccctg cgacccacac ccaccggagg acccgcgtgc tcgcagagct gaaggcagag
    13741 ctgaagtacg acctcggcca cggcgtcccc cggcggttcc tcaaccggac cctcgaggag
    13801 ttcgcccggt cggtcccggc cggcgcccgc accctggaga tcgggtgcgg ctactacgac
    13861 caccggcccc acttcgccga gcgcctgctg cgcatggacc tctacaccga gcacgagccg
    13921 gacctgaacg gtgacgcgct ggcgatcccc atcgccgacg acgccatcga cgcatccatc
    13981 tgcatctccg tgctcgagca cgtccacgac ccgtaccagg ccgtccgcga gtggcaccgg
    14041 atcatggccc cgggcggccg ggtgctggcc tggatcccgt tcttcttcgg ggtgcacggc
    14101 taccccggcg atgtcagccg cttcaccgag gaaggcgctc ggcacctgtt cgagcgggcc
    14161 gggttcgaga tcgaatcgac cgacaccgac acctactcgg gcctgttcct caacctcggc
    14221 gacgccgtgc acttcaccgt gccgcgcacc caccaccgcc cgtcggtccg ggccctgaac
    14281 cggtcgctcg ccctcgcctg ccgcgccggc ctcccgctcg accggtggct gcggctgaag
    14341 cgcctctacg ccggcacgac ggtgctcgcg gtgaagccgg gagcctcgtc ccgatgagct
    14401 ggagcagctc gctgcgggcg accgcccgtc gcaccgccag gtggcgcctc gcccggcgtc
    14461 gcacgctgcg ctggtgggtc gtgtacgggc cggtccggtc cgggacgacc tacatgatgg
    14521 aggtcatcgc cgcgaacgcc cgggtgcgcg tcagcgactg gggcctgcgc cacggactcg
    14581 gcctgccccc ggacctcccg cacatccgct tcgaccgggc ccgggcccgt gccgacttct
    14641 cccggaacgt gctggccaac gcgccggccg gcggcggccg caccctcgac ctggtctgca
    14701 agtcggccca gatgcgaccc gacgagctgc aggccctcac cgagatgtgg ggcccgccgg
    14761 agcgggtcat cttctgcctg cgggcgccgt ccggctacct cgcctcggcg gtgcggaagt
    14821 tccccgacgt ggacgtgcag gctttccgcg acgagtacct cgccgacatc gagacccacg
    14881 aggccgtggg cggggacctg ttcgtctacc gcccggacct ggacaccgag gactaccgcc
    14941 gcttcctggc gccgctcgac atcccggacg acccggacct gcggttcacc taccagggct
    15001 cgaccgccga cgacctcgtg accccggaga tggtcgacgc gttcgagcgc gtcaccggcc
    15061 tcgtccgagc acgaagccga tgacctggcg cgatcgcctc cgcggccggc gcgggcggtt
    15121 ctggagcctg cagcggctgg cccgcctcga cgccgaccag ctcgaggcgc tcgtcggcct
    15181 cgcccgcgtg gtcgggcggg agcacgtcgc gatccgccac gagagcgcag gcatcagcga
    15241 gcgggcgctg atctcgccgc tggcgtcgct ccgcttcggt gaccggctgg agatcgggcc
    15301 gaaggcgacc atcggtccct actgcgccgt gtggggtggc ttcgaggcca cgacccggat
    15361 cggcgccggt gcgctgctcg cgccgggcgc cgtggtcgtc gccgggaacc accgcatcga
    15421 cggtgagggg tgggtccgcg acctcggctt cgacgagcac gacgccagcg tcggcgaagg
    15481 ggcgtggatc ggcgcccacg ccaccatcgt cggctgccac gtcggtgccg gggcggtggt
    15541 cgccgccaac gcggtcgtgg tcgacgacgt gccagcggat gcggtggtcg ccggcgcccc
    15601 agcacgcgtg gtccggatgc gccgggccgg cgagggggtg ccgacgtgac cgggacgatc
    15661 ggggcaccgg gctcgaaggc ggacgtgtac gaggcgttcg gcaacccggc gctgctccgc
    15721 gccgtcgtgg accacctcgc cccgggcgcc gccgtgctgg acgtcgggtg cgcgtcgggt
    15781 ggcctcctcg cccggctcgc cacccacgcc ggtcgccggg aggggatcga ggtcgacccg
    15841 gtggccgccg cagcagcggc ggacgtcgcc gaccgggtgc acgtgggctc ggtcgaccag
    15901 atggcgccgg agggtccccc gttcgacgtg gtggtgctcg gcgacgtgct ggagcacgtg
    15961 cccgatccgg cggcgaccct cgatcgggtg ctggcctggc tctcgcccga aggtcgcctc
    16021 gtcatcagcc tgccgaacgt ggcccactgg tcggtgcggc tgtcgctggt cgcgggacgc
    16081 tgggagtacc gggacagcgg catcctcgac gacacccatc tgcggttctt cacgtgggcc
    16141 agcgggcgcg agctcgtcga gggcgccggc ctgcgcatcg tcgaccggcg cccggtcatc
    16201 agcggcctcg gcgcccacct cggccggcgg ctgccggcca ccgtcgaccg ggcgtggcgc
    16261 cggatcggcg tgcgccgtcc caacctgttc gcgtaccagc agatcatcgt cgcggagcgg
    16321 ggctgaggtg gtgggcgaac ccgcggtccg cctgcgtggg ctgacgaagg tctacgagcc
    16381 gagcccgcgg tggatgcagc tcatgacgcg cagcgcgtcc aagacctcga aggtcgcgct
    16441 cgacggggtg gacctcgacg tgcaccccgg caccatctgc gcggtcgtcg gaccgaacgg
    16501 ggccggcaag tcgacgatgt tccgcctcct gatgggcctg accacgccga ccgccgggtc
    16561 cgcccacgtg ctcggcttcg attgcgccaa gcggtccgag agccggcgca cgcccatcgg
    16621 gttcatgccc gccgacgagc gcagcctgtt cctgcggctc agctgcaagg agaacctggc
    16681 gttccaggcc gccctccgcg gctacggcgg ctcccgcctc gacgtccggg tggcggaggt
    16741 gctcgacatg gtcgggctct cggagagccg ggacaccgcc gccttcgcgc tgtcctcggg
    16801 catgaaggcc cggctccagc tcgctcgggc gatggtccac gagccggcgg tgctcatcct
    16861 cgacgagccg acctccacgg tcgacccggt ggcggccggc gacctgctcg agctcatcca
    16921 gcgcctcacg tccgagcagt ccctggccgt catgctcagc tcccaccgcc tcgaggagat
    16981 cgacgccctc gatgagcagg tggtactgct cgacgacggc aacgtggtgt tccagggcga
    17041 cctccgagcg ctgcggcgca tcgccgccct caccgaggtc gtgctcgagc tgtccgggga
    17101 gggggcggct caggacgtgg cggcggccct cgagctggtc cccgaggtcg catccgtggc
    17161 gcgcgacggg cgcgtcgtcg agctcacggc gaacctgacc ctcggcgcgg tcctggcgtc
    17221 gatcgggtcc cgcacggccg acgtcgtgca cgtcgacgag cggtcgagct ccacccgctc
    17281 gctgctccgc gaggtgtacg ccaacagccg ggccacgagg agcgcgtcgt gaccgcggtc
    17341 gtcgagcacg cccagctcct cggtcgctcc gcccgcgcct tctacctcgt cggcaagagc
    17401 gacgccctcg cctaccccct ggcgttcatg ctcgcgatct tctcccggct cacgaacgtg
    17461 ttcctctact acttcgtcgc ccgcatcgtc accacggacg agtcggtcgt cgccggcggg
    17521 tacttcgcgt tctccgtcgt cgggaccctc gtgctctggg tgctcggcgc cggcctcgag
    17581 ctgttcggga gcttcgtgca gcgcaccatc gaccagggtc acctcgagct ctacctggtg
    17641 cagcccgtgg cctggccgct gctgccgttc ctctggttcc agtggctcgt cgtcgaacgg
    17701 gtcgtggcgg gcatcgccac cgtggtcgtc gcctacgcgc tcggcgtccg gttcgcgacg
    17761 gaccggttgc tgctggcggt cgtggtgctc gccctcggcg tcgccgccac gcacgccatc
    17821 ggcgtcatcg cggcgagcgt gcggatgctg tccaagcgcg ccgaccccgt cctgctcgcc
    17881 tacaccgtcg cgagcacggt cttctcgggg ctgttcgtcc cggtgcaggt gctccccggc
    17941 atcctgcagt cgctcagctg gctcatcccg cacacctacg tcgtggatgc gctgcgtcag
    18001 ctgctcctcg atggcggaga ggccagctcc cgggtgtcgg tgggcaacgc cgtgctggcg
    18061 ctcggcagct tcaccgtcgt cgcctacgcc gtcggcctcg tcctgttctc caagtcgctg
    18121 cactacgcgc gccggcgagg gatcctgggc tcgtactgat gggggctggt gacgtggtcc
    18181 tgccggtcgt ggtcgtgcac taccgggcgc ccgagtggtg ccgctcggcg gtccgcgcca
    18241 tccgggcctc cacggtggcc gtcgacgtgg tcgtcgtcga caacagcggc gagctcggcg
    18301 aagccggcgt gcccggcgcc gcggtcgtcg caccggagac gaacaccggc tacgccggcg
    18361 gtgccaacgc tgggatccgg cacctgctcg ggaaccaccc cgaggcgacc cacatcgtcg
    18421 tgtgctcgca cgacttccac ccggagcccg ggtgcttcga gctgctgctc ggatccgcgt
    18481 ccgacgccgg cctcggcatc gtcgcccccc gcctcaccgg gccgaagccg agcatcggca
    18541 actggttcga cggccgacgg tcgacgaaca tcgagccccg tccggcggca accgccgtgg
    18601 tcgactcgga ttgggtgtcg ggcacatgca tggccgtgcg cgtcgacgtg ctggtcacgg
    18661 tcggcgggct ggacgagggc ttcgggtcct acatggagga cgtcgacctc tgcctgcggg
    18721 ctcggcgggc gggctggcgg gtgggcaccg tcgtcgccgc ggccgggcac gggctcggct
    18781 ccgtgtcgga cgagcggttc cgcctgacgg cggtcaacgt cgcgctgctg gccgccaagc
    18841 gggagggggc acgcgctggg tgggcgctca ccgcccgcta cgccgtgcgc tgcctgcgca
    18901 gcgtcctcgt cgccgtgctg ccgtcgaccc ggggcctggc gcggcggcgg gcgtcgctgc
    18961 gccacgcccg atcgcggggc ggcgccgtgc tcgacctcgt ccgatcccgg cgcatcggca
    19021 cctacgcgcg cgacccacac cacttcgaac cgcggttcgc cgtgcacgac aggagccacg
    19081 cgtgagtcga gccctgagcc tcgggttcga ggtccgccgc ctggtgctgg tggcccgcct
    19141 gcgcgtcctc gccgccctga agcgagccga cctggacgtc gtcatcgatc ggggggtccg
    19201 catcgagggt cgcgtgctgg tgcgcgtcca cggggggacg cgcacccgcg tgcgcatcgg
    19261 gcgcgggacc cgcatcgggg acagtgtccg cctggtcctc gacggaggcg agctcgtcgt
    19321 gggcgagcgg gtcctcctgc gcaccggggt cgtgctgcac gtgcggggtc gcctcaccct
    19381 cgaagacgag tgcctgctgt cgtactactc ggtggtccac tgcgacgagg aggtccacgt
    19441 cggcggacgg gtcggcctcg gcgagcacac cacgatctcc gactcgaccc acgtgacgcc
    19501 cccgccgggg gactggtggt accaccacat cgccaccgca cccgtccgcc tgggcgcacg
    19561 cacctggggc ggcgccaagg tcacgatcac ccggggggtc gacatcggcg ccgacgccgt
    19621 cctggccgcc ggctcggtcg tcacgtcgga tgtgccggcc ggcatgctct ccggcggcat
    19681 gccggcccgc gtgctcggtc cctcgcccct gtcgccggcg tcgcccgacc gcaccccgtc
    19741 cggcgggtga cgctcaggcc gggcaggccg gctgcacgcc ccgcacgtcg aacaccgcgg
    19801 catcccgcgc ctggaggagg ttcggtgcgc ctgccgcacc cagccaccga gcgggctcgt
    19861 cgaccagcca ccgaccgagc tcggtgccgt gcaggccccg ctcggccagc acgaccacga
    19921 cgtccagttc ggcgtcggtg accgcctgcc accagtcgcc acaggtctcg aacggcacca
    19981 gctcgtcgcc gagtcggtcg tcgccgaggg tcaccacgcg gttgtcgagc ccggtgccga
    20041 agaaggggta gctctgcggg tagccggtga caccgacggc gaggccggtg gcagcgtcgc
    20101 cggcctcgaa ggccgcccat cggggcaggt cggcggacca gcggcgatcg aggtaccgat
    20161 cgtgggccac gacggcgacc ggcagggcca gcaggaccag ggccacgccg gcgagggcgg
    20221 ctcgcggtcg aggcagcggc cgtcgtgcca gggcccagac cacgacggcg acgaccacgg
    20281 tcgccgccgc agcgagcacg gcccgccggg gatggacgcc gtgcaggttg gcggcgacga
    20341 aggcggcggc ggtgagcccg gtggccaggt gcgggcggcg acggaggccg gggtgcccca
    20401 gaaccgccag cgcgcagagc agcgccccgg gcaccgcgta gcggaggttg aactggaaca
    20461 ggaccgggtt gcccggaccg cccgccgccg acgtgggggt gacggcgtac ccgacgaggc
    20521 tgagcaggcc gcacacggcc agcagggccc actggggagc ggtgcgacgc accccggacg
    20581 tgagggcgag gaccgccgcc acggccacgg cgccgacgag gagcggccac aggggcccga
    20641 agaagccggc caggcccggg acgaagtggt cgcgccagat gtcgaggtcg gtcaggtagt
    20701 cggtcaccgg gaactcgacc gctcgctgga gctcgagctc cggtccgggc agcggtccga
    20761 aggacagcgc cggcaccggg ttcccggtcg cggccaggtt gcgcacgtac cagtagccgc
    20821 cggcgacgag agcggcgccg acgtacgtgc ccgcagcgcg cagacgggtc ccgacgggac
    20881 cggctgcgac cagggtggtc agcgccacca cggccacgac cacggtgagc ttcgtgccga
    20941 cggccgcccc ggcggccgcc ccggcgacgg ccacgagccg caggtccccg gctcggtcga
    21001 ccgctgccag cagcagggcg gcggccgcca gcaggagcgc gatcgccatc gtgtcgttca
    21061 gcgtcgatcc gctctcggcc accaccaggg ggagcgacag gacggcggcc acgatcaggg
    21121 ccccggcggc gggcaggccg gcgcgcccgc cgatggccca accggctgcg acggccatgg
    21181 ccgcgaaggc caggttgacg aagatggagg cgaagtcgga tccgagcacc gacagcccga
    21241 tgccgtgcag cagctcggcg tgcgcggggt ggtaggcggc accgtcgccg acggaggtga
    21301 accggatcgg ccacgtccgc cccgactgca cgaacagcgc cgagtgggtc aggtggtagt
    21361 gcagctcgtc ggcgtgggag aagccgcgcg ccatggtctg cagcagctcg gcgccccacc
    21421 gcaccgccac cagcccgacc gacccggcga ccagccagcg gtcccggcgg gacacggcca
    21481 cctcgtcgtc ccgggcgacc ggctgccggc ggggcatcgt cgcgatcgcg gcggcaccgg
    21541 cgatggccga gacgaccacg aggggccagg gccggaacca gccgacggtg ccgagcgccg
    21601 ctgcggcgag cagcaggccg accagggcga gcacgccggc ggagacctga cggagcggtg
    21661 accgcagcgg cggcacccac cgccggagca gggtcgtgcc gacgagccac caggctcccg
    21721 acaccagggc gaggccggcg aagccggcca cgctgcgcgt ggcgctcacc gtcaggcggc
    21781 gcgcaggtgg ctctcgtgcc gggcgtcgag catcggccgg cgtccgctgt tcaggtcgtg
    21841 gcggaagcgc caggactcgg ccagcgcctt gaccgagaag ctggccaggc gcttgagcac
    21901 cccgatggtg ccggccgacg cgaacatcgt gccggtgttc gactcgcccc ggcggacccg
    21961 gtggttgacg tccacctcca cgagggtcgc gccccgccgg gcggccagga tcgtcaggtg
    22021 cacgttgggg atgagcgagc cgtccgggat ccagcgcagc agctcgtcga gcagtggcgc
    22081 ccggtaaccg cgcagcgggc agttggcgtc acgggtcggc acgccgaaca cccggtggat
    22141 gtacacgcgc agcagccggg tcatggacat gcggaaccag gggtcgtagc ggaaccggcg
    22201 cacgccgcag acgccgtcgc cgccgtcctc cagcagcacg agcacgcggc ggatgtcggt
    22261 gccgaggaac tggccgtcgc cgtcgacgcc gatgacgtgg tcggcgccgg tgtccagcgc
    22321 ccgtcggtac gcggcgagcg cggccgggcc gtggccgcag ttggtgccgg tggactccac
    22381 gacgaggtcg gcgcgcaggc ggggccgcag cgcctcgagc acctcgacgg tgtcatcggt
    22441 ggagcagtcg tcggccacca cgaaggtgac gtcgtcgacg tgcccgtgga gcgcctcgtc
    22501 gatctcgtgg aggaacgagg cgatcccctc cgcctcgttg tacgccggga tggcgatggc
    22561 gacgctggaa gcggcgtgtt gcatcggact ccttcaggtg ctcaggatcc gacccccgag
    22621 gacgccgtcg gcacaggcga acgcccggtc gatggcctgg tcttggtcga tgtaggtgta
    22681 ggtggacaac cgaccgcaga agtgcactcg gtacttcgcc agctgctccc ggacctcctg
    22741 ctggagcgcc gcgttctggg cctcgtggcg gccgtcgacc gtgggcaccg ggtagtggcg
    22801 ggcgggcgcg ccggggtgct cctcgctcac gacggtgccg tgcacccgct ggcccgacgc
    22861 gtgcttggtc tccaccgtgc gggtgtgggg cacccgggtg ctggggcggt tcacgacgta
    22921 ggccggcgtg accgtgccgt cctcggtctc gctgggctgg tagcgagaga ccatccggat
    22981 gccccgccag gccagctcgc ccggccgtcc gaggaagtcg tcgagggggg cggtgaccac
    23041 gacatcgtcc acgtcgccga agaggtcgtc gacgtcgtcc acgccgacct cgatcccgca
    23101 cgtgaccggg accggttgca ggatcgcctc gatgatcccg ttcgcgccgt cggccgggaa
    23161 gaactcccag gtgtcccgga acaggcgggt gtagccgtcg tcgcggaggt cgacccgctt
    23221 gggggcgaac cggctggaca gctcggaggg ctcgcagccc cactgcttgc gcgtgtagcc
    23281 ctcgatgaac agctcgtaga gccgccggcc catgagggac acgacccagc tctcgaagtc
    23341 gtcgccggca gggtggggcg gcagctcggc cagctcgcgc tcgatcgcgg gccagtccgg
    23401 cagggcccgc agctcctcga tctgcggcgg ccatgacagc agccggggcg tgtcgtcgtc
    23461 gtcccgcagg aagacctcgg tgagcacccg gtgctcgtag gggcgggtca tgccgaagcg
    23521 ctggacgtac tcggccaccc gctggttcga cgtgtggaag atgtgggcgc cgttgggctc
    23581 gtacaccacc ccgttgagga cctcgacccg ggcgtggcca ccgacgacct ccccccgctc
    23641 gacgacgtgc acctcgaccc cctggtcgtg cagctcccgg gcgacgacgg cgcccgacca
    23701 accggcaccg atgacgagga ccttcatcgc agacctcctg cgggacgtgg gcgacgcgcg
    23761 acgacggcga tccggtcgcg caggtcgggc ccgaaggccc gctcggccac cgggctgcgg
    23821 ccgacgatgc cggccagcgg gcgcccgacg gccgggacga actcgccgag gcggtcggcg
    23881 tagtagcggt acgtgaacac cttcgggtgg gtgtgcacgg cggcgacctc gaagccggcc
    23941 cccgtgagca gcgccaccag cgacgcgcgc gtgaagtact ggacgtgcat cggcagcacc
    24001 gaccaccacc gccgccccag cacgcgggcg agcacgctgc cggcatcggg cacggtggcg
    24061 aacagcaacc cgcccggttc gagcagctcg gcgatgcgcc ttgctgccgc cgcgggatca
    24121 tcgaggtgct cgaggacgtc gcagcacgtg accacctgca cggacccggc gtcgagggtg
    24181 acgtcgtcga gcgtcccgac ggtcacgtcg tggccgcgct cgacggcccg ggccgcagcc
    24241 caggtggaag gctcgacgcc cgacaccgtc cacccgaggt cggcggcggc gtcgagcagc
    24301 gagccggtcc agcaaccgat gtccaggagc cggccgggtg ccgcgccgag gtggtcgcgg
    24361 acctcgagga ggtcgcggcg ggctgtcgtg gtctggccgt cggcctcctc gagcgacgag
    24421 gcatcctcga cgtgggcgta ggcagccgag aggtcgtcgg tgctcggtgg ccggtcgagc
    24481 gagccgtggc cgcacgcacg gcagcggacc acctcgccga ccgtcgcccc gaacgcggtc
    24541 gccgccggtg cgagctcgtc ggcccgcagg tccacgatgt ggtccccctg gcgacggtac
    24601 gccggcgtgc cgtgccggct cccgcacacc gggcaccccc agtcggctcc cgcctcgccc
    24661 atggccgagg accgtaatgc accgaacagg gacaattgcc atcgatggcg aagcatccct
    24721 caccacggcg cgggcggtga caggaccggc gcgcccctga ccgaccgacc gaagcgagag
    24781 acgtgctgcc gagctcagaa cagcggctcc gggcctacgc cagcgtgctg gtccgccacc
    24841 ggctggccgt catcgggacc accgtcgccg tgctcctcgt ggggttggtc ccggcgctgc
    24901 tggccgacga gacctacgcc tccacggcga gcgtccgggt ccggccggag accgcgggct
    24961 cgcccttctc cgacaccgac gagcccaatg cccagaaccg cagccgggag ctggtcaccg
    25021 acgtggaggt catccagtcc gaccgcctgc gcgacctggt gggcgaccgg gtcgacgggg
    25081 acttcggcac cgccgaggcg acgctggtgg gcttcagcga ggtgatcgag atcaccgtct
    25141 cggccggtga tcccgatctg gccgctgcgg ccgcgaacgc gtacgcggat gtgtacgtga
    25201 gcgagcgcca gcgccgggcg gtcgagaccc tggtcgacca gtccgaggag ctccgtcgcc
    25261 gctccgacga ggccgcggcc gagatggcgg ccatcgacgc ccagctgggg gcgccgggga
    25321 ccgatcccgt cacggccgag aacctccggg tccgccgggc gtccctcgcc gaccaggtgc
    25381 tggacttctc ggctcgggcc gacgagctgg aggtcgaggc cgcgctccgg gagggcggca
    25441 ccgagatcgt caacccggcc aagcccgaac ccgacccggt gagccccgac cccgtgcgca
    25501 gcgccctcat ggccggggtg ctcggcctgc tggccggcat cggcgtcgcc gtcgtcctcg
    25561 acgtggtgca ggaccgcatc tcgtcaccgg acgacctggt ggtgctcgac ccgtccctgc
    25621 cggtcctcac gtcggtgccc cacctcgctc gcgcgcccca gcgccccgtc gacctcgacc
    25681 ctcgtgggcg ggaggcgttc cgctacctgc gagcgtcgct gcacttccgc agcctcgacc
    25741 gcccgatcca gtcgctgctc gtggccagtg ccgtcgccga ggagggcaag accacgaccg
    25801 cagtcaacct cgcggtggcg tgcgcggaag cgggcgcccg ggtggtgctc gtcgacgccg
    25861 atctgcgccg cccgtcggtg caccagtgct tcgacctcga gctcgagccc gggctcaccg
    25921 acgtcctcgc cggccgggtg accttcgccg acgcgatcac ctacgtcgag ccgaacctcg
    25981 ccgtcctccc ggccggcgca tctgccgccg gatcccatga gctgctcgag cggcccgcct
    26041 tcgcgcagct cctccaggcc gcagcgaaga agtccgatct ggtcgtggtg gatgcgcccc
    26101 cggtgctgca ggccgccgat gccctcgtcg cggcccgtgc catcgacgcc gcgctcgtgg
    26161 tggccagggt cggacaggtg cggcggcggg aggtgcgggc gctgctccgc cggctccggg
    26221 acgcccggat cccgctcatc gggttcgtgg ccaacggcgg caccgccgag gtctcctacg
    26281 gcgagtacgc gccgctcgcc ggagaccgcg cctgactcaa ccgccgacgg gctccggggg
    26341 ccggtacccg tggagctcga tctcgggggc gaacagccga gccacgcgtg cgatctggcc
    26401 ctccgtgagc gcggtcggca cgccggatcg acggcggtcg ccgtagaccc tcggcagctc
    26461 cggtacgggc aggccgagct cgtcgagcag gaccggcagc tgctcgtcca ggtgctcgta
    26521 ccgcaggatg cggtcgacga tcacctcgcc gttcgacggg tcggtgtagc tgcggtggtt
    26581 gtacggggac acgccggtgc cgtggtagcc cttggcgatg cggtcctcga cgaagtcgag
    26641 gtaggtgtcg acgtcccagt cctggccgta ctgggtgcgg acccaggaca gccccgacag
    26701 caccttgtcc cacgggttcc gctcgacgca gaacgtcgtg tactcccgcc actcgcgggg
    26761 ggcgcgggcc cggatgatcc aggccggggt gtggccaccg aacttcttgc gccggagcag
    26821 catctggcgg gtgccggccg cgtccaggac gatgccggcc cgtcgggcga tccgcagctc
    26881 ggggagcggg ttggcccggc cgaggtggtt ccgcggcgcc aggtcgtggg acgggtccat
    26941 cgtcggcggg accacgtcct ccgggccgca gagcttcgcc agcgcgatct tcagcgacgt
    27001 gccagcggtc ttgccggtct tgacgaagac gaaccggtgg cggtgggaga ggatcacctc
    27061 ccctccgttc tacgaccggg gacccttccc ctgcggtggg ccgctcttgt cgcccttgcc
    27121 gctgttgccc cgggaggtcg acgggtcggt cggctcctcc accaccggct ccggcgcggg
    27181 gtcagcggtt gcggtgccgt cgcccgtcag cgagttccac tcggcgatgg agaaggcctt
    27241 gccgtagatg atgatcgcct cggcgatcga cgggtggtcc cagatcgtgc ccgaggcccg
    27301 cacctcgcct gcgctgagct tcgcacgcca ctcggcctgg gtcagcaccg gggagaacac
    27361 gaggggggag ttcgtcgcat cgctctggag cacgttgtcg cgcagcgtcc agtcggtgaa
    27421 gtcctggacg agctcgggcg tgccggtgac gtggttcgtc caggtgcgca ggcgctcccc
    27481 ggagaagacg agctgcccgt aggggttgtc ggccagggtg gacccctcga gggtcacgcc
    27541 gccggcggag ctgacgatga ccccggcccg ggcgttgccg cagaacgtgc tgttgcggat
    27601 cgtgatgggg ccggcgagcg cctcgaggaa cacgccgtct cgggcgttgc cgcacgagcg
    27661 gtcgccgtcg acgaggacgt cgaccacgtc ggtgtcgaac cacaggcccc gggcccagtt
    27721 gccgtcccag acgttgtccc ggaagacggc gccgtggagg agcatgttct tggagccggc
    27781 gacggaccag ccgaggtagc cgttgcgggc accccggacg ttgttgccgg tggcgacgtt
    27841 gccctgcacg accacgtcgt gggcccggta cgtgtcgatg ccgttgccgc cgttggcctc
    27901 ggagacgttg tcccgcaccg tcacgccctc ggtgcagcag acgaacagtc cgccccagct
    27961 cgagtggcgg aacgtgttct gctcgacgac cacgtcggtt gactcggaca cgttggccat
    28021 gaacttggtg aagggggcgg cacccgcctg gaacgtgaag ccgcggacgg tgacgttggc
    28081 ggcgccgttg ccgatgtcga gcacccgggc ccgcgtggcg acctccacga ggttcccgct
    28141 caggtcgggg ctggccccgg cgggcgggtg ggcgtagatg gcgtcgcccg tctcgtccac
    28201 caggaacgaa cccgggacca ggtcctcgaa ccgcagcacc tggacgaggg gttcgccgtc
    28261 gacgaacatc gcttcccgac gccgcacgcc gtcgctcggc gccaccgcgt cgccccagct
    28321 ccccgggatc ggggccaggc cccagttggc cgtccacggc gcccgcgcca gcggggtgcc
    28381 cgcgaccggc gtccagcggt cctcgacgtc ggcgccggtg atcaccgcgc caccgggctc
    28441 ttcggactcg agcaccagcg gtgcgtcggc ccccgtcccg gtgatcttca cgcgctcccg
    28501 gtaggtgccc ggagccacga cgatgcgcac cggctggccg gcggcccgga gcgactcggt
    28561 ggcggcgatg gcccagccga cggtggggaa cgggagctcg agcgagccgt cgggccagac
    28621 gtcctggacg gagaacgagc cgtcgacgcg gaaggtgagg gcggtggcgg ggagcggctg
    28681 ctcggtggcg ccgtcggccg ggtcgccgct cggctgcgcc tctgcggatg ctgacgggac
    28741 ggccagcacg gcgagcaggg cggcgagcgc caccatggtg agcaggcgga ggcggtgcgg
    28801 caggagcgaa cgcatccgac cagtatcgct ttgtccgatt cgtccgcaca tggacacatc
    28861 gggccatttc cgcatggcgc ggtcggtgcg ctgggtgtca accccagaag ttgacggtca
    28921 cccagagctc gccggcgccg tcgcgcacgc agcccacagc ggcgtgggtg agcgccccgg
    28981 acaggatctt gtcccggtgc gagggggagc ggaggaactc gtcgtacacg gcccgggcgc
    29041 tgccgttgcc ccagcccacg ttctcggccg cggtcatggc ctcggcccgg gcggcgaaga
    29101 cctggtcgcc gtagcggggg ttgtgcgcca gctcgctccg gtccgccatc tcgtggctcc
    29161 agtcgacggc gacgtggagg atggcgtcat cccaggcgag cgcgtggcgt ccggcgccgg
    29221 cccgggtctc gttcatccac cgcagcatcg aggactcgca ctcggcgtcg cggctgccgg
    29281 tcctggcggc cgcaggttgc ggcggtgggg gcgctggtgc cgtcgtggcg accggggccg
    29341 gggcgggcgc cggtgcgaac gtgggcggcg gtgctgtcgg gctcggggcg gccgcaggtg
    29401 cctgggtcgg atcgggagca ggggccgggg ccggggccgg cggtggtggt tcgggggcct
    29461 gctcggtcgc tgcagccggc gggggtgcca gcgggaccgc cggctcaccc gcggcggcgg
    29521 agagcacccg ctgggcatcg ggcgaggcga cgacctcggc ccgctccagc gcggcgtcgg
    29581 cccacaccgg gttggtgacc acgccggcgg acacgatgcc ggcggatgcg acgacggcga
    29641 tggcgaccgc gagcgctcgg cgcagcatcg gctccttcga catggcgacc cccggcgtcg
    29701 ggccggcacg gttgccggct ccgctggttc ttcgccatcg ctcccggatc ccctgccacg
    29761 gggcggagcc ccgtcctcga ccgcctcggg gtcagcctgc cggggggagc agctcggcga
    29821 gcagcgcccg ggcccggcga tcgatgtcgt cgacgatggg acggacctcc tcgaccggct
    29881 tgccggccgg gtcggtgagg tcccagtcga ggtagcgctt cccggggaag atcgggcacg
    29941 cgtcgccgca ccccatggtg acgatcacgt cggcggcgcg ggcggcctcg tcggtgagcg
    30001 gcttggggaa ctccttggac acgtccaggc cccgctcggt gaggatctgg gccacggccg
    30061 ggttgatgtc gtcaccgggc gcggacccgg cggagcggac cgtcacccgc ccctggccgt
    30121 ggtggtcgag caggacgcgg gcggcgacgg agcggccggc gttgtgggtg cacacgacga
    30181 ggatctcggg tgcgggggtc gtgtcggtca cgaggggtct ccggtggggt agtagcggcg
    30241 gcgggcccac agggccacgt agacgagccc gacgagcacg ggcacctcga tgagcgggcc
    30301 gaccacgccg gcgagggcct ggccggaggt gacgccgaag acgctgatgg ccacggcgat
    30361 ggcgagctcg aagttgttgc ccgccgcggt gaaggcgacg gtggtgttgc ggtcgtaggg
    30421 aaggcccatg cgcaggccga tggcgaacga cgacccccac atgatggcga agtagatgag
    30481 cagcggcagg gcgatgcggg ccacgtcgaa gggctcggac gtgatggcgt cgccctgcag
    30541 cgcgaagagc accacgatgg tgaacagcag gccgtacagg gcgaccgggc cgatgcgggg
    30601 caggaacgtg ccctcgtacc actccgtgcc cttgcgccgc tcgccgatca ggcgggtgag
    30661 gaacccggcc agcagcggga tgcccaggaa gatgagcacc gacttggcga tctcccagat
    30721 cgacacgtcg agggcctcct ggtcgaagcc cagccagctc gggagcagct ccaggtagaa
    30781 gtagccgagc agcgagtagg cggcgatctg gaacaccgag ttcaccgcga ccaggaaggc
    30841 ggccgcctcg cggttgccgc aggccaggtc gttccagatg aggaccatgg cgatgcagcg
    30901 ggccaggccg acgatgatca ggccggtccg gtactcgggc aggtcgggca gcagcagcca
    30961 ggccagggcg aacatcagcg ccgggccgat cacccagttg aggaccagcg acaggccgag
    31021 catgcgccga tccgcggtga cctcgccgag gttgccgtag cggaccttgg ccagcaccgg
    31081 gtacatcatc agcagcaggc cgatggcgat gggcagcgag accgtgtcga tcttgatgcg
    31141 gtcgagggtc tcgtcgaggc cggggaacag cttgccgagg gcgatgccga gcgccatggc
    31201 agcgccgatc cacacgggca ggaaccggtc gagcgtggag agcttctcga ggaccggggc
    31261 gtcctcggga cggggttcgg tgacgacggt catgtgcagg cccgctggcg ttcgcccggt
    31321 gccgggcagc acgacgtgat cgaacggagc tggccgccga gcgcctcgag ctggtcacgg
    31381 cggagccggt agtacgtcca ctgccggtgc ttctcggact cgaccaggcc ggcgtcgcgg
    31441 agcaccttca ggtggtgcga caccagcggc tgggccatgt cgaggtcttc agccagatgg
    31501 cagacgcaca gctcctcgca ggagagcagc tccacgatcc gccaccggtt gggctcggcc
    31561 agcgccttca gcaccgccgt cggtggcgcg gccacctcca catcaacagt catgcataga
    31621 cattagcgtt gatgtccgtc cggggaggac cttgggctct ggttcctggt cctcggtgcg
    31681 gggatgctcg ccccatgagc cgtcctcgcg tcgtcatcct gtccggtgcc cgcaccccga
    31741 tcgggaagct gtccggctcc ctgtcgtcgt tctcggccat ggagctgggc ggcttcgcgg
    31801 tctccgaagc actgacgcgg ggcggcctgc gcggcgagca ggtcgactac gtctacatgg
    31861 gccacgtgct ccaggccggc gcaggccaga tcaccgcccg ccaggcggcc gtgaacggcg
    31921 gcatcccgat ggacgtgccg gccaccacgg tgaacaaggt gtgcctgtcc gggatggacg
    31981 ccatctacca ggccgaccgg cgcatccggg ccggtgacgc cgagatcgtc atggccggcg
    32041 gcatggagtc gatgaccaac gccccgcacc tgctgcccgg ggcgcgggcc ggctaccgca
    32101 tcggcgacgg caccatcgtc gattcgatga tgtacgacgg cctgtactgc gcgttcgatt
    32161 cgtgcgccat gggcgagggg accgagcgct actcccaggc ccacggcatg gagcgcgagc
    32221 acatggacgc catggccgcc gaatcccacc gccgagccga cgccgcgcag caggcgggtc
    32281 tcttcgagca cgagatcgtc cccgtcacgg tcccgcagcg gaagggcgac gcgttgaccg
    32341 tgacccatga cgagggcgtg cggggcgaga ccaccatcga gtcgctcgcc gggctgcgac
    32401 cggccttcga tcgcaacggg cacatcaccg ctggcaacgc cagccagatc agcgacgggg
    32461 cgtccgccac cgtcatcacg acgctcgagg tcgccgagcg cctcggcgtg agcccgctgg
    32521 cggagatcct cggctacggg caggtggcgg gccccgatgc ctcgctgctc caccagccct
    32581 cccgggcgat caaggacgcc ttgaagcgga ccggcctgtc cctcgccgac ctcgacctgt
    32641 tcgagatcaa cgaggccttc gccgcggtgg gcgtcgcctc ggccgacgac ctcggcatct
    32701 ccagggaggt cgtcaacgtg aacggcgggg ccatcgccgt cggccaccca ctcgggatgt
    32761 cgggcaaccg catcgcgctg cacctcgccc tcgagctcgc ccggcgcggc ggtggcaccg
    32821 gcgccgcagc cctctgtgga ggcggcgggc agggcgacgc cgtcatcgtc cgcaccatga
    32881 gctgatcgga gccacgtgtc cgctcccagc gccgacccag gccgcaccta ccaggcccac
    32941 gccgagaccg agggcacggg cgtgggccag atccgcacga agcgggccgt cgtgcggttc
    33001 gactcgtcgc cgtcgcaggg cgacgagctg cccgggccgg cggacctgct cacctccgcg
    33061 ttcgcggcct gcatcatcaa gaacgtcgag cggatgggcg agatgctccc gttcgcctac
    33121 gacgtcgcat cggtcgaggt gacggccgag cgccaggacc gtccgccgaa gatcgtgcgg
    33181 atccgctacg tggtcaccgt cgtcaccgac gagcccccgc accgcgtgaa cctgctgcac
    33241 cgcaacatcc agcgccacgg gaccatcttc aacaccctcg ccgccgtgtg cgaggtggcc
    33301 ggcaccatcg aggcgcggcc gagccctcgc gtcggcgatg cgcacccggg gtgaaggccg
    33361 ttcggtgatc cgtcgtctca gtcgggcttc gtggccttga tgatggcgct gtggacgccg
    33421 tcatccacgt cgttggtgac ggtgacgcgc acgtcggtga acccggcagc ggtgagaccg
    33481 gcttcgtact cggtgatccg gagcgcgccc gcgatgcatc cgacgtagga gccggtcgcg
    33541 gcccggtcgt cgtcagagag gtcgtcgtcg gtgacgacgt cgctcacgcc gatgtgcccg
    33601 ccgggacgga gcacccggaa catctcgtcg aagacggcgt gcttgtcggc agcgaggttg
    33661 acgacgcagt tggagatgat cacgtcgacc gacgagtcgg gcagcgggac ctgttcgatg
    33721 gtgccttcga ggaactcgac gttcgtggct cgggcttccg ccgcgttgcg gcgggcgagg
    33781 tcgagcatct ccggggtcat gtcgagcccg tacacgtagc cggttgggcc gacccggcgt
    33841 gcggacagca gcacgtcgat gccgccgccg gaaccgaggt cgaggacggt gtcaccgggt
    33901 tggagatcgg ccaccgcggt cgggttgccg cagccgaggc tggcgaggac agcctccgcg
    33961 gggaggtcct cgtcgaggtg gtcgtagtgc ttcgtgcccc acggctcgtc gtccgggctg
    34021 gaacacgacg gatcggggct ggtcgcggcc tgcgcgaagt gcgctcggac ctgggtgtgg
    34081 atcgcgtcg
//
LOCUS       DATISU010000004         8589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_512670, whole genome shotgun sequence.
ACCESSION   DATISU010000004 DATISU010000000
VERSION     DATISU010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512670"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1769
                     /locus_tag="VLR27_00350"
     CDS             <1..1769
                     /locus_tag="VLR27_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP01918.1"
                     /translation="GSLATLLRSRSRRLLGSLVAPHRRAMGWATVLIAVNMVAHLSGP
                     WFVGQASDEGIPPLLEGGSGSIRPLLTVVGAYLVATVVAAATFNAFLLVSGRIGQEVI
                     VDIRRRLARHFTTLSLSFHQRYTSGRVISRQTSDVDAIAELLGHGLLQLTTSSLTLVG
                     ITIILVVLDPLLALAALSVVPILFLLTRWFRHHSERAYRATREAIALVIVHFVESLGG
                     IQAVQSFRRERRNQEIFDDLDGRYRDANVWSARLAAIYGPGVQFAGRATVAVVLLYGG
                     TRVLDGAIEIGVLAAFVLYLRRFFEPLTELSQFYNLFQAAAAATEKLSGVLDEEPTVA
                     PPVDPVPLPEAAGAITFDGVRFAYGDEVVLHDLDLEISAGQTVAVVGQTGAGKSTIAR
                     LAARFWDPTVGTVRLDGVALTDLAEDDLRRAVVVVTQESFLFSGTVADNIEFGRPGAG
                     RAQIEAAAEAIGADDFIRGLPQGYDTDIQAKGARLSAGQRQLLAFARAFLADPAVLIL
                     DEATSSLDIPSERLVQRALRTLLADRTAIIIAHRLTTVEIADRILVVDGGRIVEDGTP
                     ADLRTGAGAYGDLHRAWLDSLI"
     gene            1787..2233
                     /locus_tag="VLR27_00355"
     CDS             1787..2233
                     /locus_tag="VLR27_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSP01919.1"
                     /translation="MHGPPTSSVGVDLLLRSIRRRYRQVEPGQLAAEVGAGSLVVDIR
                     PVAQRARDGALLGSVVIDRNVLEWRLDPTSPHRIDGVAPDRRIVLVCNEGFSSTLAVG
                     TLLDLGRPDVTDLRGGFQGLLAADRVPASGPEPPDLSPPPPPRRWI"
     gene            2325..2951
                     /locus_tag="VLR27_00360"
     CDS             2325..2951
                     /locus_tag="VLR27_00360"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HSP01920.1"
                     /translation="MPRFTAENTILAKEIIGRYPHPKSALIPLLHLAQEQDGYLAEDA
                     MVHIAELIDHTPAEVIGTASFYEMFKREPVGDYLINVCTNIACMLMGGEELLHHLEDR
                     LDIRPGATTADGMFTLEDVECIAACTEAPCLTVNYRYFHKISTDEADQLLEDLRAGRR
                     AHEIPKHGTLARTRQAIPADRRAGAAVPGECGQPVWMTPKDEAEDAPA"
     gene            2948..4315
                     /gene="nuoF"
                     /locus_tag="VLR27_00365"
     CDS             2948..4315
                     /gene="nuoF"
                     /locus_tag="VLR27_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HSP01921.1"
                     /translation="MSDQSTSRTVPQMITGRFQYDDAHTIERYQATEGYAALRKALQM
                     HPEDVHAEIKEASLLGRGGAGFPAGVKWGFCPPGVWPRYFVVNGDESEPGTYKDRLLM
                     ERDPHQLIEGVLIGAYATGCAQAFLYVRGEMALAQERIAAALNEAYAAGLVGKDILGS
                     SFSCDIVLHWGAGAYIVGEETALIESLEGNRGMPRLKPPYFPAAKGLYMQPTIVNNVE
                     TMANVPWIIRNGAEAYTAFGAESSRGTRMFAVSGHVKKPGVHEVEFGTTTFRDLIYGH
                     CGGIRGNHTLKAFIPGGASAPWFYEEHLDLPLEAGAVGAAGSMLGSGAIVVMDETTDA
                     VKAAWRVVRFFARESCGKCTPCREGTNWLEKVLRRMLDGQGRPEDIGLLLDLCDNISP
                     SIAWPPKQTTICPLGPSAVSPIASAIMRFKSEFQAYCGGGDVPTSVTINGKAYLPPEG
                     APAGV"
     gene            4308..7058
                     /gene="nuoG"
                     /locus_tag="VLR27_00370"
     CDS             4308..7058
                     /gene="nuoG"
                     /locus_tag="VLR27_00370"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HSP01922.1"
                     /translation="MSEQPVKITIDGREIGAEDGELVIAAAERTGTYIPRFCWHPRMN
                     PVGMCRMCLVEIDTGRGPALQPSCMIPASEGMTVVTDSDMVKKAQEGVLEFLLANHPL
                     DCPVCDKGGECPLQDQAYSHGPGESRFVEEKVHFEKPIAISDLVDLDRERCILCDRCT
                     RFADEVAGDPLIHFIDRGAQTQINTFPDEPFASYFSGNTVQICPVGALTSRAYRFKAR
                     PWDLDQVESTCTSCSVGCRVAVQSSRNQVLRYQGVDVDAVNWGWLCDKGRFDFESIHA
                     EGRLGEPLLRADEGHVTATWSDALGRAAGAIEAALASSGPSGVGVIGGARLTNESAYA
                     WAKLAKGVIGTDNVDAQLGDGLPAEAVLGLPRATIDQACAPGGTVVVLGVDPKEELPV
                     LYLRLRHAVVEDGVTLVEVVPHATGVSGLAAHSLVHGPGAVADVVTQMLAESPTDAGG
                     IGAAALAAAGAAVREGPVTVILGRGNLADRAELVASAASRLAEGLDDVRFLSGLRRGN
                     VHGALAAGLAPGMLPGSVSLDDGREWFHDHWSTVPSDRGLDTEGILGAAADGKLDVLV
                     LLGADPLSDFPDHDLARRALAGARTVVAVDTLPNASVRQADIVLPAAGYAETDGTTTN
                     IEGRISRLSQKVTPPGTARADWVIAAELAYRLGDDLGLESLDDVFAELARVAPSHHGL
                     TATDVGVDGVLVPLSRREAGSEARTAEDPIGLQTAGGDEEPGADVAPEAAEAAAEAEA
                     ADTEAGEADAEAEATEAQADAEAADAATPDEGDGAEPVADDEGEPAGPGLPSPVRFSA
                     ATVQVPSPDTYSLRLLTTRKLYDLGTTTQASRSLAKLAPGAVLHLHPHDFDRLGIEVD
                     GQVTVSNREGGRGSVTVPVRPSATVPKGAALLAFLQPGGHAAELVDASAVVTDVRVEV
                     AR"
     gene            7055..8284
                     /gene="nuoH"
                     /locus_tag="VLR27_00375"
     CDS             7055..8284
                     /gene="nuoH"
                     /locus_tag="VLR27_00375"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HSP01923.1"
                     /translation="MIGAIYALDPLYLDGVGLAEVLITLGKVLVTFLILLLSVLFMVW
                     FERKVIADMQNRVGPNRAGPFGLLQTLADGLKLIVKEDLIPNRSRRFVFALAPFLSVI
                     PAFLVFAVVPLGGTFSEGDPGVVTIAGHETFAQLADPPIGVLWILLVSSIGVYGVMLA
                     GWSSGSKYPLLGSVRATAQMVSYEAAMGLSVATLVLTTGALSTRTMVAVQAGEGFAGF
                     VPNWNVILTGIVPFAIFFIAALAETNRPPFDLVEAEQELVGGFHTEYSSIRFGMFFVA
                     EFMHPVTMSAVIVTLWFGGPAGPLLPGFLEPVSGIFWLVAKVFVFLYIFVLIRGTLPR
                     FRYDQLMDLGWKVLIPLQLGWLLLLAALNVGRDEGWNYAVVIGIGFFVVGGATFFLLS
                     RASEVSRQRRELGEVDA"
     gene            8284..>8589
                     /locus_tag="VLR27_00380"
     CDS             8284..>8589
                     /locus_tag="VLR27_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HSP01924.1"
                     /translation="MGYLDGFFITIKKTFAKGSQSGENVTSPYVKDEGGKRAKPIRTH
                     GRHVLNKYEDGMEKCIGCELCAGVCPARCIYVRGADNDPADPVSPGERYGFIYEINYL
                     "
BASE COUNT         1164 a         3378 c         2795 g         1252 t
ORIGIN      
        1 agggcagcct ggccaccctg ctgcggtcgc gcagccggcg gctcctcggc tcgctcgtcg
       61 ccccccaccg gcgcgccatg ggctgggcca cggtgctgat cgccgtgaac atggtcgccc
      121 acctgtccgg gccctggttc gtgggccagg ccagcgacga ggggatcccg cccctgctcg
      181 agggtggcag cggctcgatc cggccgctgc tgaccgtggt cggcgcctac ctcgtcgcca
      241 cggtggtcgc cgccgccacg ttcaacgcct tcctgttggt ctcgggccgc atcggccagg
      301 aggtcatcgt cgacatccgc cgccggctcg cgcgccactt caccaccctc agcctgagct
      361 tccaccagcg ctacacgtcg ggccgggtga tctcgcgcca gaccagcgac gtcgacgcca
      421 tcgccgagct cctcggccac ggcctgctgc agctcaccac gtcctcgctg acgctcgtcg
      481 gcatcaccat catcctcgtc gtcctcgatc cgctgctggc gctggcggcg ctgtccgtgg
      541 tgccgatcct gttcctgctg acccgttggt tccgccacca ctccgagcgg gcctaccggg
      601 cgacccgcga agccatcgcg ctggtcatcg tccacttcgt cgagtccctc ggcggcatcc
      661 aggcggtgca gtcgttccgc cgggagcggc gcaaccagga gatcttcgac gacctcgacg
      721 gccgctaccg cgacgcaaac gtgtggtcgg cccgcctcgc cgcgatctac gggcccggcg
      781 tgcagttcgc cggccgggcc acggtcgccg tcgtgctgct ctacggcggc acccgggtcc
      841 tcgacggggc catcgagatc ggggtgctgg ctgcgttcgt gctctacctg cgccggttct
      901 tcgagccgct gaccgagctg agccagttct acaacctgtt ccaggctgcg gccgccgcca
      961 ccgagaagct gtcgggcgtg ctcgacgagg agcccacggt cgcgccgccg gtcgatccgg
     1021 tgccgctccc cgaggccgcc ggcgccatca ccttcgatgg tgtgcgcttc gcctacggcg
     1081 acgaggtcgt cctccacgac ctggacctcg agatctccgc cggacagacc gtcgccgtgg
     1141 tgggccagac cggtgcgggc aagtccacca tcgcccgcct cgccgcccgc ttctgggatc
     1201 ccacggtggg caccgtgcgc ctcgacggcg tggcgctcac cgatctcgcc gaggacgacc
     1261 tgcgccgggc cgtggtcgtg gtcacccagg agagcttcct gttcagcggg acggtggccg
     1321 acaacatcga gttcggccgc cccggcgccg gccgggccca gatcgaggcc gccgccgagg
     1381 ccatcggcgc cgacgacttc atccggggcc taccccaggg ctacgacacc gacatccagg
     1441 ccaagggtgc ccggctgtcc gccggccagc gccagctgct ggccttcgcc cgggcgttcc
     1501 tcgccgaccc ggccgtgctg atactggacg aggccaccag ctccctcgac atccccagcg
     1561 agcggctcgt gcagcgcgcg ctgcgcacgc tgctggccga ccggaccgcg atcatcatcg
     1621 cccaccgcct cacgacggtg gagatcgccg accggatcct cgtggtcgac ggcggccgca
     1681 tcgtcgagga cgggacgccg gccgatctcc gaaccggggc cggggcctac ggcgacctgc
     1741 accgggcctg gctcgactcg ctcatctagg ccgctaccgt cgagccgtgc acggtccgcc
     1801 gacgtcctcc gtcggggtcg acctcctgct ccgctcgatc cggcgccgct accgccaggt
     1861 cgagcccggc cagctggccg ccgaggtcgg cgccggctcg ctcgtggtcg acatccgccc
     1921 ggtcgcgcag cgggcgcggg acggggccct gctcgggtcc gtggtgatcg accgcaacgt
     1981 gctcgagtgg cgcctcgacc ccacgagccc gcaccgcatc gacggggtcg cgccggaccg
     2041 ccgcatcgtg ctcgtgtgca acgaggggtt cagctcgacc ctcgcggtgg ggacgctgct
     2101 cgacctgggc cgtcccgacg tcaccgacct gcgaggcggg ttccaggggc tgctcgcggc
     2161 cgatcgcgtg ccggcatcgg gccccgagcc ccccgacctg agcccaccgc ccccgccacg
     2221 acgctggatc tgacccgagc cggaggccag gggtagcgtg cccgattcgc ggcccatccc
     2281 cgacgttcgg gcgtcctcgg acgcttgtgc aaggattcac gacgatgccc cggttcactg
     2341 ccgagaacac gatcctcgcc aaggagatca tcgggcggta cccgcatccc aagtcggcgc
     2401 tgatccccct cctgcacctc gcccaggagc aggacgggta cctggcggag gacgcgatgg
     2461 tccacatcgc cgagctcatc gaccacacgc cggccgaggt catcggcacc gccagcttct
     2521 acgagatgtt caagcgcgag ccggtggggg actacctcat caacgtgtgc accaacatcg
     2581 cttgcatgct catgggcggc gaggagctgc tgcaccacct cgaggaccgc ctcgacatcc
     2641 gcccgggcgc caccaccgcc gatggcatgt tcaccctcga agacgtggag tgcatcgccg
     2701 cgtgcaccga ggcgccgtgc ctcaccgtga actaccggta cttccacaag atcagcaccg
     2761 acgaggccga ccagctcctc gaggacctgc gcgccgggcg ccgggcgcat gagatcccca
     2821 agcacggcac gctcgcccgc acccgccagg ccatcccggc cgaccgccgc gccggtgccg
     2881 ccgtccccgg cgagtgcggc cagcccgtgt ggatgacgcc caaggacgag gcggaggacg
     2941 ccccggcatg agcgaccagt ccacctcgcg caccgtgccg cagatgatca cgggccgctt
     3001 ccagtacgac gacgcacaca cgatcgagcg ctaccaggcc accgagggct acgccgccct
     3061 gcgcaaggcg ctgcagatgc accccgagga cgtgcacgcc gagatcaagg aggcgtcgct
     3121 cctcgggcgc ggcggcgccg gcttccccgc cggcgtcaag tggggcttct gcccgcccgg
     3181 cgtgtggccc cggtacttcg tggtcaacgg cgacgagtcc gagcccggca cctacaagga
     3241 ccgcctcctc atggagcgcg acccccacca gctgatcgag ggcgtgctca tcggcgccta
     3301 cgccaccggc tgcgcccagg cgttcctgta cgtccggggc gagatggccc tggcccagga
     3361 gcgcatcgcc gctgccctga acgaggccta cgccgccggc ctggtcggca aggacatcct
     3421 cggctcgagc ttctcctgcg acatcgtcct gcactggggt gcgggcgcct acatcgtcgg
     3481 tgaggagacc gcgctcatcg agtccctcga gggcaaccgg ggcatgcccc gcctgaagcc
     3541 gccgtacttc ccggccgcca agggcctgta catgcagccc accatcgtca acaacgtcga
     3601 gacgatggcc aacgtgccgt ggatcatccg caacggcgcc gaggcctaca ccgcgttcgg
     3661 cgccgagagc agccggggca cccgcatgtt cgcggtgtcc ggccacgtga agaagcccgg
     3721 cgtgcacgag gtcgagttcg gcaccaccac cttccgcgac ctcatctacg ggcactgcgg
     3781 tggcatccgg ggcaaccaca cgctcaaggc gttcatcccc ggtggcgcat cggccccgtg
     3841 gttctacgag gagcacctcg acctgcccct cgaggccggc gccgtcggcg cggccggctc
     3901 catgctcggc tccggcgcca tcgtcgtgat ggacgagacc accgacgccg tcaaggcggc
     3961 gtggcgggtg gtgcggttct tcgcgcgcga gtcctgcggc aagtgcacgc cgtgccgcga
     4021 gggcaccaac tggctcgaga aggtcctgcg gcgcatgctc gacggccagg gtcggcccga
     4081 ggacatcggc ctgctgctgg acctgtgcga caacatctcg ccgtccatcg cctggccgcc
     4141 caagcagacc accatctgcc cgctcggtcc gtccgcggtc agcccgatcg ccagcgcgat
     4201 catgcggttc aagtccgagt tccaggccta ctgcggcggt ggcgacgtcc ccacctcggt
     4261 gaccatcaac ggcaaggcct acctgccgcc cgaaggagcc ccggccggtg tctgagcagc
     4321 ccgtgaagat caccatcgac gggcgcgaga tcggcgccga ggacggcgag ctcgtcatcg
     4381 ccgccgccga gcgcaccggc acctacatcc cgcgcttctg ctggcacccc cgcatgaacc
     4441 cggtcggcat gtgccgcatg tgcctggtcg agatcgacac cggccgcgga ccggcgctgc
     4501 agccctcctg catgatcccg gcgtccgagg gcatgaccgt ggtcaccgac tcggacatgg
     4561 tgaagaaggc ccaggagggc gtgctcgagt tcctcctcgc caaccacccc ctcgactgcc
     4621 cggtgtgcga caagggtggc gagtgccccc tgcaggacca ggcgtactcc cacggccccg
     4681 gcgagtcccg gttcgtcgag gagaaggtcc acttcgagaa gccgatcgcg atctccgacc
     4741 tcgtcgacct cgaccgcgag cgctgcatcc tctgcgaccg ctgcacccgc ttcgccgacg
     4801 aggtcgccgg cgacccgctg atccacttca tcgaccgcgg cgcccagacc cagatcaaca
     4861 cgttccccga cgagcccttc gcctcgtact tctcgggcaa caccgtccag atctgcccgg
     4921 tcggcgccct cacctcccgg gcgtaccggt tcaaggcccg gccgtgggac ctcgaccagg
     4981 tcgagtccac gtgcacctcc tgctcggtcg gctgccgggt cgccgtccag tccagccgca
     5041 accaggtgct gcgctaccag ggcgtcgatg tcgacgccgt caactggggc tggctctgcg
     5101 acaagggccg cttcgacttc gagtccatcc acgccgaggg tcgcctcggc gagccactcc
     5161 tgcgcgccga cgagggccac gtcaccgcca cctggtccga cgccctcggt cgcgccgccg
     5221 gcgccatcga ggccgccctc gcctcctcgg gcccgtccgg ggtcggcgtc atcggcggtg
     5281 cccgcctgac caacgagtcg gcctacgcct gggccaagct ggccaagggc gtcatcggca
     5341 ccgacaacgt cgacgcccag ctcggcgacg gcctgccggc cgaggccgtg ctcggcctgc
     5401 cccgcgccac catcgaccag gcctgcgccc ccggcggcac ggtcgtggtc ctcggtgtcg
     5461 accccaagga ggagctgccg gtcctgtacc tgcgcctccg ccacgccgtc gtcgaggacg
     5521 gcgtcaccct cgtcgaggtc gtgccccacg ccaccggcgt ctcgggcctc gccgcccact
     5581 cgctcgtgca cggccccggt gccgtcgccg acgtcgtgac ccagatgctc gccgagtcgc
     5641 ccaccgacgc cggcggcatc ggtgccgccg cgctcgccgc cgccggcgcc gccgtgcggg
     5701 agggtccggt caccgtcatc ctcggccgtg gcaacctcgc cgaccgggcc gagctcgtgg
     5761 cctcggcggc cagccgcctc gccgagggcc tcgacgacgt ccgcttcctg tccggcctgc
     5821 gccggggcaa cgtccacggc gccctcgccg ccggcctggc acccgggatg ctccccggca
     5881 gcgtctccct cgacgacgga cgcgagtggt tccacgacca ctggtccacc gtcccgagcg
     5941 accgcggcct cgacaccgag gggatcctcg gcgccgccgc cgacggcaag ctcgacgtcc
     6001 tcgtgctgct cggtgccgac ccgctgtcgg acttccccga ccacgacctc gcccgccggg
     6061 cgctcgccgg cgcccgcacc gtggtggccg tcgacacgct gccgaacgcc tcggtccgcc
     6121 aggccgacat cgtgctgccc gccgccggct acgccgagac cgatggcacc accaccaaca
     6181 tcgaaggtcg catcagccgc ctgtcgcaga aggtgacgcc ccccggcacg gcccgcgccg
     6241 actgggtcat cgctgccgag ctcgcctacc gcctgggcga cgacctcggg ctcgagagcc
     6301 tcgacgacgt gttcgccgag ctggcccggg tcgccccctc gcaccacggc ctcaccgcca
     6361 ccgacgtcgg cgtcgacggg gtgctcgtcc ccctgtcccg ccgcgaggcc ggctccgagg
     6421 cccgcacggc cgaggacccg atcggcctgc agaccgccgg tggcgacgag gagcccggcg
     6481 ccgacgtggc accggaggcg gccgaggccg ccgccgaagc cgaggccgcc gacaccgagg
     6541 ccggtgaagc cgacgccgag gccgaggcca ccgaggccca ggccgacgcc gaggccgctg
     6601 acgccgccac ccccgacgag ggcgacggcg ccgagccggt cgccgacgac gagggcgagc
     6661 cggccggacc gggcctgcct tcacccgtgc ggttctcggc cgcgacggtc caggtcccgt
     6721 caccggacac ctactcgctg cgcctgctca ccacccgcaa gctgtacgac ctcggcacca
     6781 ccacccaggc gtcgcgctcg ctggcgaagc tggcgcccgg cgcggtgctc cacctgcacc
     6841 cccacgactt cgaccgcctc ggcatcgagg tcgacggcca ggtcaccgtg tccaaccgcg
     6901 agggcggccg gggctcggtc accgtgcccg tccgcccgtc ggcaaccgtg cccaagggcg
     6961 cggcgctgct cgccttcctc cagcccggcg gccacgccgc cgagctcgtc gacgcctcgg
     7021 ccgtggtcac cgacgtccgc gtggaggtcg cccggtgatc ggggccatct acgcgctgga
     7081 ccccctctac ctcgacgggg tcggcctcgc cgaggtcctc atcacgctcg gcaaggtgct
     7141 ggtcaccttc ctgatcctgc tgctctcggt cctgttcatg gtctggttcg agcgcaaggt
     7201 catcgccgac atgcagaacc gcgtcggccc caaccgggcc ggccccttcg gcctcctgca
     7261 gaccctggcc gacggcctga agctgatcgt caaggaagac ctcatcccca accggtcccg
     7321 gcgcttcgtg ttcgcgctgg ccccgttcct gtcggtcatc cccgccttcc tcgtgttcgc
     7381 cgtcgtcccc ctcggtggca ccttcagcga gggcgacccc ggcgtcgtca cgatcgccgg
     7441 ccacgagacc ttcgcccagc tggccgaccc gcccatcggc gtgctctgga tcctgctcgt
     7501 gtcctccatc ggcgtctacg gcgtcatgct cgccggctgg tcgtcgggct cgaagtaccc
     7561 gctgctcggc tcggtgcgcg ccaccgccca gatggtgtcc tacgaggcgg ccatgggcct
     7621 ctcggtggcc acgctcgtgc tgaccaccgg tgcgctgtcc acccgcacca tggtcgcggt
     7681 gcaggccggc gagggcttcg ccgggttcgt gcccaactgg aacgtcatcc tcaccggcat
     7741 cgtccccttc gccatcttct tcatcgccgc gctggccgag accaaccgcc cgccgttcga
     7801 cctcgtcgag gccgagcagg agctcgtcgg cggcttccac accgagtact cctcgatccg
     7861 cttcggcatg ttcttcgtcg ccgagttcat gcacccggtc accatgtcgg cggtcatcgt
     7921 caccctgtgg ttcggcggtc ccgccggccc gctcctgccg ggcttcctcg agccggtgtc
     7981 gggcatcttc tggctcgtcg ccaaggtgtt cgtcttcctc tacatcttcg tgctcatccg
     8041 cggcacgctg ccccggttcc gctacgacca gctcatggac ctgggctgga aggtcctgat
     8101 cccgctccag ctcggctggc tcctgctgct ggccgcgctc aacgtgggcc gggacgaggg
     8161 ctggaactac gccgtcgtca tcgggatcgg gttcttcgtc gtggggggcg cgacgttctt
     8221 cctgttgagc cgagcatccg aggtctcgcg gcagcgccgc gagctgggag aggtggacgc
     8281 ctgatgggat acctcgacgg gttcttcatc acgatcaaga agaccttcgc caagggcagc
     8341 cagtccggcg agaacgtcac gtcgccgtac gtgaaggacg agggcggcaa gcgggccaag
     8401 ccgatccgca cccacggccg ccacgtcctc aacaagtacg aggacggcat ggagaagtgc
     8461 atcgggtgcg agctgtgcgc cggcgtgtgc ccggcccgct gcatctacgt gcgcggcgcc
     8521 gacaacgacc cggccgaccc ggtgagcccc ggcgagcgct acggcttcat ctacgagatc
     8581 aactacctg
//
LOCUS       DATISU010000005         2220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_512671, whole genome shotgun sequence.
ACCESSION   DATISU010000005 DATISU010000000
VERSION     DATISU010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512671"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VLR27_00385"
     CDS             complement(<1..662)
                     /locus_tag="VLR27_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSP01925.1"
                     /translation="MSDVLLTERRGRVLVMTLNRPDQRNALNAELADALKASLEELDA
                     DGELTAGVLTANGKNFCAGMDLKEFATKGPPASMGAIYNQPWQKPLIGGVHGPVLAGG
                     LELALVCDLLVAAEGSSFGVPEVKRGLLAAGGALVRLPSRLPYAVAMEMSLTGDPISA
                     DQAEQYGLVSKVVPQEQVLDEAIALAERIAANAPLSIAASKQMIRNSAGRTEEEGWAE
                     QGP"
     gene            complement(715..1647)
                     /locus_tag="VLR27_00390"
     CDS             complement(715..1647)
                     /locus_tag="VLR27_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HSP01926.1"
                     /translation="MARIVVTGGAGFLGSHLCDRLLERGDEVVVLDNLITGNVANIEH
                     NFGHPGFTFVEHDVSTFIWVPGEVDAVLHFASPASPKDYLEMPIQTLKVGSLGTHNCL
                     GLAKEKGATFFLASTSEVYGDPQVHPQTEEYWGHVNPVGPRGVYDEAKRFAEAMTMAY
                     HTHHGLDVRIVRIFNTYGPRMRPKDGRVVSNFVMQALQGVPITIYGDGSQSRSFCYVD
                     DEVRGFLALLDSDQIGPINIGNPGEFTMVELAELVLELTGSSSEIVHEPLPEDDPTQR
                     KPDITKARELLGWEPEVELREGLQRTIEHFARLV"
     gene            complement(1648..>2220)
                     /locus_tag="VLR27_00395"
     CDS             complement(1648..>2220)
                     /locus_tag="VLR27_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HSP01927.1"
                     /translation="GIGYDKRIGSEFLRPGHGFWGSCFPKDTRAMVKIAEEGGYDFHL
                     LKGVIQVNDEQYERTAEKAIELMGGAVDGKQVAAWGLTFKARTDDLRESPSLEVLGRL
                     AARGAKVRAYDPAVSHPLEGIEVVSDPYDACDGADVLLVLTEWDEFKWLDLGEVKARM
                     ATPAVVDARNLLDRSALIRRGFTFRGIGRR"
BASE COUNT          329 a          736 c          804 g          351 t
ORIGIN      
        1 gggccctgct cggcccagcc ctcctcctcg gtgcgaccgg cggagttgcg gatcatctgc
       61 ttggaggcgg cgatggacag cggcgcgttg gcggcgatgc gctcggccag ggcgatggcc
      121 tcgtcgagca cctgctcctg cgggacgacc ttgctgacca ggccgtactg ctcggcctgg
      181 tcggcgctga tgggatcgcc ggtgagcgac atctccatgg ccacggcgta gggcaggcgg
      241 ctcggcaggc gcacgagggc accgccggca gccagcaggc cccgcttgac ctcgggcacg
      301 ccgaagctgg agccctcggc agcgaccagc aggtcgcaca ccagggccag ctcgaggcca
      361 ccggccagca ccgggccgtg cacgccaccg atgaggggct tctgccacgg ctggttgtag
      421 atcgcaccca tcgacgcggg cgggcccttg gtggcgaact ccttgaggtc catgccggcg
      481 cagaagttct tgccgttggc ggtgaggacg ccggccgtga gctcgccgtc ggcgtcgagc
      541 tcctcgagcg acgccttcag ggcgtcggcc agctcggcgt tgagcgcgtt gcgctggtcg
      601 gggcggttga gggtcatgac caggacccgg cctcggcgct cggtcagcag gacatcggac
      661 acgtggggtg ctccttcagg gagtcggcaa cagacgttgc caaggtacgc gccgctacac
      721 gagtcgggcg aagtgctcga tcgtgcgctg caggccctcg cgcagctcga cctcgggctc
      781 ccagccgagc agctcccgtg ccttggtgat gtcgggcttg cgctgggtgg ggtcgtcttc
      841 ggggaggggc tcgtggacga tctccgacga ggacccggtg agctcgagca ccagctcggc
      901 cagctccacc atcgtgaact cgccggggtt gccgatgttg atggggccga tctggtcgga
      961 gtcgagcagg gcgaggaacc cgcgcacctc gtcgtcgacg tagcagaacg accggctctg
     1021 ggagccgtcg ccgtagatcg tgatcgggac cccctgcagc gcctgcatga cgaagttgga
     1081 gaccacccgc ccgtccttgg gccgcatccg agggccgtag gtgttgaaga tgcgcacgat
     1141 gcgcacatcg aggccgtggt gggtgtggta cgccatcgtc atcgcctcgg cgaagcgctt
     1201 ggcctcgtcg tagacgccac ggggaccgac cgggttcacg tgcccccagt actcctcggt
     1261 ctgggggtgg acctgcgggt cgccgtagac ctcggacgtg gaggcgagga agaaggtggc
     1321 gcccttctcc ttggccaggc ccaggcagtt gtgggtgccg agggagccga ccttcagggt
     1381 ctggatcggc atctccaggt agtccttcgg cgacgccggc gaggcgaagt gcagcaccgc
     1441 gtcgacctcg cccggcaccc agatgaacgt cgagacgtcg tgctccacga acgtgaaccc
     1501 ggggtggccg aagttgtgct cgatgttggc gacgttgccg gtgatcaggt tgtcgaggac
     1561 cacgacctcg tcgccccgct cgaggaggcg gtcgcagagg tgggagccga ggaagccggc
     1621 gccaccggtg acgacgatgc gggccactca gcgccgcccg atgccacgga aggtgaaacc
     1681 ccggcggatg agcgcagagc ggtcgagcag gttgcgggcg tcgacgacgg caggcgtcgc
     1741 catgcgggcc ttcacctcgc ccaggtcgag ccacttgaac tcgtcccact cggtgagcac
     1801 caggagcacg tcggcgccat cgcaggcgtc gtaggggtcg gacacgacct cgatgccctc
     1861 gagcgggtgc gagacggccg ggtcgtaggc ccggaccttc gccccgcgtg cggcgaggcg
     1921 cccgagcacc tcgagcgagg gcgactcgcg caggtcgtcg gtgcgggcct tgaacgtgag
     1981 gccccacgcc gccacctgct tgccgtcgac ggcgcccccc atcagctcga tggccttctc
     2041 ggctgtgcgc tcgtactgct cgtcgttgac ctggatgacc cccttgagga ggtggaagtc
     2101 gtagccaccc tcctcggcga tcttcaccat cgcccgggtg tcctttggaa agcatgaacc
     2161 ccagaagccg tgtcctggcc gcaggaactc cgagccgatc cgcttgtcgt acccgatgcc
//
LOCUS       DATISU010000006         4473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_233250, whole genome shotgun sequence.
ACCESSION   DATISU010000006 DATISU010000000
VERSION     DATISU010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233250"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VLR27_00400"
     CDS             <1..451
                     /locus_tag="VLR27_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HSP01928.1"
                     /translation="GLDVPVERITIRGRAVRKVHEQLREEMEDFADRLGVDTAELAIG
                     GRSFGGRMCSMLVAAGEPVAGLVLLSYPLHPPGKPDDLRVDHFADITVPTLFVSGTKD
                     PFASPEELEHHAGAITGPVDFVWLDGEPHSPRDDAAVVAAVAGWLGG"
     gene            complement(480..1076)
                     /locus_tag="VLR27_00405"
     CDS             complement(480..1076)
                     /locus_tag="VLR27_00405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP01929.1"
                     /translation="MTSWMEIVAENPGHSSWYIERFRTMAANGDDLEGEARFVDAMVP
                     RGSRILDAGCGPGRVGGALFQRGHDVVGVDVDPALIEAAEHDHPGPTWLVGDLAELDL
                     PARGIDGGFDVIVCAGNVITFVPADDRRPALEHMGHHLADEGRIAIGFGDHRGYGFDE
                     FRADVAAAGLEVEIELSTWDLRPFRQSSDFLVALLRRT"
     gene            complement(1087..1746)
                     /locus_tag="VLR27_00410"
     CDS             complement(1087..1746)
                     /locus_tag="VLR27_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HSP01930.1"
                     /translation="MQGTAPAEQALPRPRLRGLIHRYASVAFAIGFIVLVVAGDPAER
                     PWLAVYGLAVTTMLAVSAAYHSGRLSPAAQVVFKRIDHSTILLAIAGSYTGIVGLGLD
                     GSSRTRMLVLVWILAVIGMAIRMLWLHAPYPIVAVVYLAVGWVALIDIDALTDALTGT
                     ELALVVLGGVLYSVGAAVYALHAPNPWPATFGYHELFHLLVVLAAAVHLAAVASLVAS
                     G"
     gene            complement(1749..3278)
                     /locus_tag="VLR27_00415"
     CDS             complement(1749..3278)
                     /locus_tag="VLR27_00415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP01931.1"
                     /translation="MTTVEVTDTVDDEGLAAFLAPRDDVVREVAPTGPPDDLCYGLDV
                     GPFQRYERRLRVRPAADGRHEVIQTTDFALGIGFWSFLFLPLIRRATRRPGGKSPWWA
                     PPQVLDAQAGASLASLASLSLVGGYLGTLITQTLTYAADEFEVGERAQSDTFAAVRIG
                     VLLAMAVAAWADRNGRRRALLWCAAAGIVATSLGALAPNMVVLGGTQLVARGLSTALA
                     LLIVVVVAEEMPAGSRAYGVSLLTMAAGLGAGNVLWLLPLVELDERAWRILYVVPLLF
                     LPLVKITAPLLPESRRFVRRHATARFAGHGRRLALLAGSALALAAFSSPASQLQNEYL
                     REDFGFSAAQITAFTILTITPGGLGIVLGGRLADVRGKRLIGGIGVAGGTIFTALMYL
                     TDSPPLLWAASAVGSVIGGLAVPALSVYGPELFPTSLRTKANVAITVLGVSGSVVGLL
                     IAGRLAERWSLGPGVAALSVGPAIVVLLLLPRYPETAHRELEELNPEDALGVPGAGGI
                     L"
     gene            3447..3704
                     /locus_tag="VLR27_00420"
     CDS             3447..3704
                     /locus_tag="VLR27_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HSP01932.1"
                     /translation="MEEQIQKVIEAIRPAVQADNGDIFLRSVDTETGIVEVELVGACV
                     SCPASTLTLKAGVERILKDRVEGVTEVRNIGETFIESSVSL"
     gene            3716..>4473
                     /gene="meaB"
                     /locus_tag="VLR27_00425"
     CDS             3716..>4473
                     /gene="meaB"
                     /locus_tag="VLR27_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HSP01933.1"
                     /translation="MTGPGRDAEQLMDAAAGGDRRSVARLLSMVERGGDDARAAGALA
                     HARSGAAYTVGITGAPGAGKSTLTSALLGELRSRGERVSVLAIDPSSPFTGGAILGDR
                     IRMQDHALDDGVFIRSMATRGHLGGLALATPQAVRVLDAAGFPWVLVETVGVGQVEVE
                     IVGAADTTVVVVNPGWGDAVQANKAGLMEIADVFVINKADRPGAEETRRDLDGMLDLT
                     DYGEWRPPIVDAVATEGTGVSELWEQILAHRAHL"
BASE COUNT          676 a         1594 c         1668 g          535 t
ORIGIN      
        1 gggcctcgac gtgccggtgg agcggatcac catccggggc cgggccgtgc gcaaggtgca
       61 cgagcagctg cgggaggaga tggaggactt cgccgaccgc ctcggcgtcg acaccgcgga
      121 gctggcgatc ggcgggcgct cgttcggcgg gcgcatgtgc tcgatgctgg tggccgccgg
      181 tgaaccggtg gccgggctcg tcctgctcag ctacccgctg cacccgccgg gcaagcccga
      241 cgacctgcgc gtcgaccact tcgccgacat caccgtgccg accctgttcg tgtccggcac
      301 caaggacccc ttcgccagcc ccgaggagct cgagcaccac gccggcgcca tcaccggccc
      361 ggtcgacttc gtgtggctcg acggggaacc ccactcgccg cgagacgacg ccgcggtggt
      421 ggctgcggtc gctggctggc tgggcggctg acgcgcgaca ggggcactgc cagttcgggt
      481 caggtccggc ggaggagagc aaccaggaag tccgagctct ggcggaacgg gcgcaggtcc
      541 cacgtcgaca gctcgatttc gacctcgagc ccggccgcgg cgacgtcggc gcggaactcg
      601 tcgaacccgt agccgcggtg gtcgccgaag ccgatggcga tgcgaccctc gtcggccagg
      661 tggtgaccca tgtgctcgag ggctggacgg cggtcgtcgg ccgggacgaa ggtgatgacg
      721 ttgccggcgc agacgatgac gtcgaacccg ccgtcgatgc cccgtgccgg caggtccagc
      781 tcggccaggt caccgaccag ccaggtcggg ccggggtggt cgtgctcggc ggcctcgatc
      841 agcgccgggt cgacgtcgac ccccacgacg tcgtggccac gttggaacag cgcgccgccg
      901 acgcgacccg gaccgcagcc ggcgtcgagg atgcgcgagc cccgcggcac catggcgtcc
      961 acgaagcggg cttcgccttc gaggtcgtcg ccgttggcgg ccatcgtgcg gaaccgctcg
     1021 atgtaccagc tcgagtggcc ggggttctcg gccacgatct ccatccacga cgtcatggcc
     1081 ccagtcctac ccgctggcga ccagcgacgc cacggcggcg aggtgcacgg ccgcggccag
     1141 caccaccagc aggtggaaca gctcgtggta cccgaacgtc gccggccagg ggttcggggc
     1201 gtgcagcgcg tagaccgccg cgccgaccga gtacaggacg cccccgagca ccacgagggc
     1261 cagctcggtg ccggtgagcg cgtcggtgag ggcgtcgatg tcgatcaggg ccacccaccc
     1321 cacggccagg tacaccacgg ccacgatcgg gtacggggcg tgcagccaca gcatgcggat
     1381 cgccatgccg atgacggcga ggatccagac caggaccagc atccgcgtgc gcgacgagcc
     1441 gtcgagcccg agcccgacga tgccggtgta ggagccggcg atcgccagca ggatcgtcga
     1501 gtggtcgatc cgcttgaaga cgacctgggc ggcgggggac agccggccgc tgtggtaggc
     1561 cgcgctcacg gcgagcatgg tggtgaccgc cagcccgtag acagcgagcc agggccgctc
     1621 ggcggggtcg ccggcgacca cgagcacgat gaacccgatg gcgaacgcga cggatgcgta
     1681 gcggtggatg aggccgcgga gccgtggtct cggcagcgcc tgctcggccg gtgcggtgcc
     1741 ctgcatgctc acaggatgcc tcccgcgccg ggtacgccga gggcgtcctc ggggttcagc
     1801 tcctcgagct cgcggtgcgc ggtctcgggg tagcggggga gcagcagcag gaccacgatg
     1861 gcgggcccga ccgagagggc ggccacgccc gggcccagcg accaacgctc ggccaggcgg
     1921 ccggcgatca gcagaccgac caccgaaccg gagaccccga ggacggtgat ggccacgttg
     1981 gccttcgtgc gcagggacgt cgggaacagc tcgggcccgt agaccgagag cgccggcacg
     2041 gcgaggccgc cgatgaccga gccgacggcg gaggcggccc acagcagggg cggggagtcg
     2101 gtgaggtaca tgagggccgt gaagatcgtc cccccggcga cgccgatgcc gccgatgagc
     2161 cgcttgccgc gcacgtcggc caggcgccca ccgaggacga tgccgagccc gcccggcgtg
     2221 atggtgagga tggtgaaggc ggtgatctgg gcggcggaga agccgaagtc ctcccgcagg
     2281 tactcgttct ggagctggga ggccggcgag ctgaaggcgg ccagcgccag cgcggagccg
     2341 gccagcaggg cgaggcggcg gccgtgcccg gcgaagcggg cggtggcgtg gcggcgcacg
     2401 aaccgtcgcg actcgggcag cagcggtgcc gtgatcttca ccagcggcag gaacagcagg
     2461 ggcacgacgt agaggatccg ccacgcccgc tcgtccagct ccacgagcgg cagcagccac
     2521 agcacgttgc cggcacccag gccggcggcc atcgtcagca ggctgacgcc gtaggcccgc
     2581 gagccggccg gcatctcctc ggccacgacc accacgatca gcagggcgag cgccgtcgac
     2641 aggccgcggg cgaccagctg cgtgccgccg agcacgacca tgttcggtgc cagcgctccg
     2701 agggaggtgg ccacgatgcc cgcggccgcg caccacagga gcgcgcggcg gcgaccgttg
     2761 cggtcggccc aggccgccac cgccatcgcc aggagcacgc cgatgcgcac ggcggcgaac
     2821 gtgtcgctct gggcccgctc accgacctcg aactcgtcgg cggcgtaggt cagcgtctgg
     2881 gtgatgaggg tccccaggta cccgccgacc agggagagcg acgccagcga cgccagggag
     2941 gccccggcct gggcgtcgag cacctggggc ggcgcccacc acgggctctt gccgcccggt
     3001 cgccgggtcg ctcgccggat cagcggcagg aacaggaacg accagaagcc gatcccgagg
     3061 gcgaagtcgg tggtctggat gacctcgtgg cgcccgtcgg ctgccgggcg cacccgcagg
     3121 cggcgctcgt agcgctggaa cgggccgacg tcgaggccgt agcagaggtc gtcgggggga
     3181 ccggtcgggg cgacctcgcg caccacgtcg tcgcgtgggg ccaggaacgc cgccagcccc
     3241 tcgtcgtcga cggtgtccgt gacctcgacg gtcgtcatcc ggccgcgtcg ggggaggggg
     3301 caccggtgcg ggtcaccggc acactgtccc attcgcggcg tgatcgctca cagccatgcg
     3361 ggaacaggcg gggccgccac gttgttcagc aagggtgccc ctcgccagac tgagggaccc
     3421 ccgcccccgt gaggagcccc gttccggtgg aagagcagat ccagaaggtc atcgaagcca
     3481 tccgtccggc ggtgcaggcc gacaacggtg acatcttcct gcgcagcgtg gacaccgaga
     3541 cgggcatcgt cgaggtcgag ctcgtcggcg cgtgcgtgtc gtgccccgcg tccaccctca
     3601 cgctcaaggc cggggtcgag cgcatcctga aggaccgcgt cgagggcgtc accgaggtgc
     3661 gcaacatcgg cgagaccttc atcgagagct ccgtcagcct gtagaagttc ggtccatgac
     3721 cggacccgga cgggacgccg aacagctcat ggacgccgcc gccggcggcg accgccgatc
     3781 ggtggcgcgc ctgctctcca tggtcgagcg gggtggcgac gacgcccggg ccgccggcgc
     3841 cctcgcccac gcccgcagcg gcgcggccta caccgtcggc atcaccggcg cgcccggtgc
     3901 cggcaagtcc acgctcacct ccgcgctgct cggcgagctc cggtcccggg gcgagcgggt
     3961 ctcggtgctg gccatcgacc cgtcctcgcc gttcaccggc ggcgccatcc tcggcgaccg
     4021 catccgcatg caggaccacg ctctcgacga cggggtgttc atccgctcga tggcgacgcg
     4081 gggtcacctc ggcgggctcg ccctcgccac cccccaggcc gtgcgcgtcc tcgacgccgc
     4141 cggcttcccg tgggtgctgg tcgagacggt cggcgtgggc caggtcgagg tcgagatcgt
     4201 cggcgccgcc gacaccaccg tcgtggtcgt gaaccccggc tggggcgacg ccgtccaggc
     4261 caacaaggcc ggtctcatgg agatcgccga cgtcttcgtc atcaacaagg ccgaccgacc
     4321 cggcgccgag gagacccgcc gcgacctgga cggcatgctc gacctgaccg actacggcga
     4381 gtggcgtcca ccgatcgttg acgcagtggc caccgagggc accggggtct cggagctgtg
     4441 ggagcagatc ctcgcccacc gcgcccacct cga
//
LOCUS       DATISU010000007         9732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_421, whole genome shotgun sequence.
ACCESSION   DATISU010000007 DATISU010000000
VERSION     DATISU010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9732
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1047
                     /locus_tag="VLR27_00430"
     CDS             136..1047
                     /locus_tag="VLR27_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSP01934.1"
                     /translation="MDLLIVILGAALVVGAVGVLVWALTSERELDTAQANLGRGGMLT
                     DSRQIALSASAKTRILDPFFSAVGNRARGFTSAGMGDRVQRKLDLAGLYEKGWTVERM
                     LVIRFLALAVGAGLIVLVLAGGTGRQSLGLAALIAALAYLGPEAWLDRKAGERQGLIE
                     KQLPDVIDQLTVSVEAGLGFDAAMARSAEGRTGPLADELARVLQDLQVGVDRQVALDR
                     MVERTDVPDLKAFVVAIRQSTRHGLPIARVLHVQSQELREKRRARVEEKAAQLPVKIV
                     FPLVFCILPSLFVIILGPAGINIMDSF"
     gene            1068..2240
                     /gene="ribF"
                     /locus_tag="VLR27_00435"
     CDS             1068..2240
                     /gene="ribF"
                     /locus_tag="VLR27_00435"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HSP01935.1"
                     /translation="MQIVRDTLSCPELGQGTAVTIGAYDGLHLGHRAVIAEVQREASA
                     RGLASAVVTFDRHPASVVRPESAPKLLCDLDQKLELLESTGIDLVVVIRFDEQRADET
                     ADEFVQEVLVDCLKARTVIVGADFHFGKGRGGDVALLNRLGPDLGFDVHGMALVDVDG
                     RPTADDRRVSSTAIRRALVAGEVEAAAGMLGRPHEVRGVVHQGDQRGRDLGFPTANVA
                     VPGSILLLADGIYAGWFVRADGQVLPTAIRRALVAGEVEAAADMLGRPHEVRGVVHQG
                     DQRGRDLGFPTANVAVPGSILLPADGIYAGWFVRADGQVLPTAISLGRRPTFYETADA
                     SLLEAHVLDFSGDLYDERVAVRFVTRLRGEERFDTVEDLIEQMQRDCEDARRILAS"
     gene            2237..3484
                     /locus_tag="VLR27_00440"
     CDS             2237..3484
                     /locus_tag="VLR27_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSP01936.1"
                     /translation="MTGTGFPDDVTIIPVTVGHVDPLEPVYDDDESREDRSHLLPRER
                     ARRALSSVLPEAEMPVVGPESAGAAERGLVAAPRLARHVFRLSDGHEVGVTVSGRGIP
                     LVMVHGFTAEGFLYAQTLSRLVSMGFKVVAIDTANHGSTQGLPSGGANFREYATLLGR
                     ALDELGIEQAFFVGHSMGGRVITQLCAAEPERALGIILIDAIVGDAWDRLVNVGRLFP
                     PAMAGVVVLLALDAMTTPPVLKNPEQALKLGRLLAPTLVGHAVRPWRLLGPAVSILRS
                     RGSRWMLQRLAQEQIPVVAVHGTRDFAVPLQTAKDAARQARGEFVVIDGANHCWPLRD
                     PETLPAIVSDLLHGSFGEAYRQAVRDHGLEPDLARLDEVERAFYPPDAPIRDMTPKLE
                     FARLTARRRRPAYSYDIKPALPQ"
     gene            3673..3945
                     /gene="rpsO"
                     /locus_tag="VLR27_00445"
     CDS             3673..3945
                     /gene="rpsO"
                     /locus_tag="VLR27_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HSP01937.1"
                     /translation="MSEQIDRLKPELIAEHRLHESDTGSPEVQIAILTKRINHLNDHL
                     STHKKDHHTRRGLLKLVGRRRRLLDYVRKNDVERYRAIIATHGLRR"
     gene            4118..6598
                     /locus_tag="VLR27_00450"
     CDS             4118..6598
                     /locus_tag="VLR27_00450"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HSP01938.1"
                     /translation="MTTEPTRVSAPIPGADKELVFETGKLAQQSQGAVLASIGGTKTL
                     VTANAAKSVREGMDFFPLTVDVEERMYAAGRIPGSFFRREGRPTDLAILTCRLTDRPL
                     RPSFPAGYRNETQIVSTIMGSDGRNPYDVLTINGASAALMLSGLPFDGPIGAVRMAFD
                     IDGEWVPHPEHDEIEESTFDLVVAGREAEDGSIAIMMVEANGTEKAWQHYEAGAPFVT
                     EEAIAQGLEESKKSIKDSIDAQRKLVEEYGARPPLEWSAADDYQDDVLEKVREVGTDR
                     LREVGSIAIKADREAAERAVKAEIRDELSEEFAGRDKEIGNAIKTVLKDVIRSRIVNE
                     GVRIDGRGPADLRPVTAEVGILDTAHGSGLFQRGETQVLNVLTLGMPKMDQLLDNLGP
                     QEKKRYMHHYNMPPHANGETGRVGSPKRREVGHGLLAERALLPVIPTMDEWPYTFRLV
                     SEVLASNGSTSMASVCSSSLSLMDGGVPIKAAVAGIAMGLVYADGKYTTLTDILGAED
                     AFGDMDFKVAGTRDFITALQLDTKIDGLPADVLADALAQARDARNTILDIMDECIAEP
                     RDDVQGSAPKIISFEIPVDKIGEVIGPKGKVINQIQGETGADIGVDDDGVVGVVTIGG
                     TDKGAVEEARRQIELIVNPPSAHPGEIYMGKVVNITKFGAFVNILPGRDGLLHISKIG
                     GKRRIDKVEDELELGQEIEVRVDDIDPNGKISLSPASEPADEGGDDDGPKRERSDRGG
                     RDGGRGRSSDRGDRGGSKDSKEDTGGTAAGGTASSGEFVSFEDAFDEELKEEFGDLGP
                     EGSSGGGDGGNRGGGRGRGRSGGGGRRR"
     gene            6649..7875
                     /locus_tag="VLR27_00455"
     CDS             6649..7875
                     /locus_tag="VLR27_00455"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSP01939.1"
                     /translation="MANGVRVVSDVMPHARSVTTGVWVAVGGRDEPAELAGASHFLEH
                     LFFKGTEARSARSIAEAVDAVGGDMNAFTAREHTTFVTRLPAAHWRLGMELIGDILSA
                     PALRPHEIDAEREVILEELWAALDTPEDRVHTLSMEALFPGHALGREVLGDEDTVRAM
                     TRDDILGFFETHYRPANLVVAAAGKVDHDELCEVVSERLGGGDGGAEPARHAPTPDVD
                     DLRTQVRDGEQVHVILGWRGLPLDHEDRYALHVADQVLGGGSSSRLFQAIREERGLAY
                     SVYSWVSSYSDAGLLGVYAGTSPGRSPQLLDALESEIDTIRTGGVTADEVRVATGYLA
                     GATELGLEDSGSRMTRLGRMLLSTGSVVSIDEQLERLHAVTVDDVNAVLQQVLSAPRS
                     VAAVGPVDTTRLARAV"
     gene            8032..8826
                     /gene="dapB"
                     /locus_tag="VLR27_00460"
     CDS             8032..8826
                     /gene="dapB"
                     /locus_tag="VLR27_00460"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HSP01940.1"
                     /translation="MTIRVGVFGAGGRMGSTVCAAVAADRECELVAAVDPHHEGIDLH
                     LVARGTDLPPLTVAGGAEEFERAEVEVVVDFTHLEAACDNLAWAAEHGVHAVVGTTGF
                     SAADLARFESTFTDSNCMIVPNFAIGAVLMMRFAEMASPFFQTAEVLEYHHDEKVDAP
                     SGTAMLTVERMAAASEEWAEDPTRKEVVAHARGGEGPGGIHVHSVRMRGMVAHQEVLL
                     GTEGQTLSIRHDSYDRSSFMPGVLLAVKAIGDRPGLTIGLDEVLGL"
     gene            complement(8887..>9732)
                     /locus_tag="VLR27_00465"
     CDS             complement(8887..>9732)
                     /locus_tag="VLR27_00465"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSP01941.1"
                     /translation="LNVVLSALLLVPIFWAIRTFDTWWLVGGLLFLGIALFLNFVYPV
                     LIMPRFNKFTPMEPGPLRTRIEEIAVLADTSIEGVYTMDGSKRSRRGNAFVAGFGPTK
                     RVVVYDTMMEFPLEQIAQVVAHEIGHYRLNHIPKTFPLAGLQMLITLAFVQYVIGSDT
                     VLGWAGVDDLGDPGAAPLFLLGLGLPMAALGLVSSWVSRRNEREADLEALELLGDPTS
                     FVAMWPSFVALNKADLEPGWWDKLNASHPGVAERMQFGLDWAELNGVPAERPDKAAVP
                     EPTEP"
BASE COUNT         1450 a         3668 c         3244 g         1370 t
ORIGIN      
        1 tctacagctc caaccccgat tacatggaac cgctgttcag ccgcaccatc gggctgatca
       61 tgctcggcgt cgccgtggcg ctgctcgtcg ccggtgcgct gtggctgaag aagctgatcg
      121 acgtggagta ctgagatgga cctgctcatc gtcatcctcg gagccgccct cgtcgtcggg
      181 gccgtcggcg tcctggtctg ggcgctcacc tccgagcggg agctcgacac cgcccaggcc
      241 aacctcggtc gcggcggcat gctcaccgac agccgccaga tcgccctgag cgcctcggcc
      301 aagacccgca tcctcgatcc cttcttcagc gcggtcggca accgggcccg ggggttcacc
      361 tccgccggca tgggcgaccg cgtccagcgc aagctcgacc tggccggcct gtacgagaag
      421 ggctggacgg tggagcggat gctcgtcatc cggttcctgg ccctcgccgt cggtgccggg
      481 ctcatcgtgc tggtcctcgc gggcggcacc ggtcggcagt cgctcggcct ggccgcgctc
      541 atcgccgcgc tggcctacct cggccccgag gcgtggctcg accgcaaggc cggtgagcgc
      601 cagggcctca tcgagaagca gctgcccgac gtcatcgacc agctcaccgt ctcggtggag
      661 gccggtctcg gcttcgacgc cgccatggcc cgctcggccg agggccgcac cggccccctc
      721 gccgacgagc tggcccgcgt cctccaggac ctgcaggtcg gcgtcgaccg ccaggtcgcc
      781 ctcgaccgca tggtcgagcg caccgacgtg cccgacctca aggcgttcgt ggtggccatc
      841 cgccagtcga cccgccacgg cctgcccatc gcgcgcgtgc tccacgtcca gtcccaggag
      901 ctgcgcgaga agcgccgggc ccgggtcgag gagaaggcgg cccagctgcc ggtgaagatc
      961 gtgttcccgc tggtgttctg catcctgccg tcgctgttcg tgatcatcct cggcccggcc
     1021 ggcatcaaca tcatggactc gttctaggca caagtaccct cgggctcgtg cagatcgtcc
     1081 gcgacacgct cagctgcccc gagctcgggc agggcaccgc ggtcacgatc ggcgcctacg
     1141 acggcctcca cctgggacat cgcgccgtca tcgccgaggt ccagcgcgag gccagcgccc
     1201 ggggcttggc gtcggccgtc gtcaccttcg accggcaccc cgcatcggtg gtgcgcccgg
     1261 agtcggcgcc gaagctgctc tgcgacctgg accagaagct cgagctgctc gagagcaccg
     1321 gcatcgacct cgtcgtcgtc atccgcttcg acgagcagcg ggccgacgag accgcggacg
     1381 agttcgtcca ggaggtcctg gtcgactgcc tcaaggcccg cacggtcatc gtcggcgccg
     1441 acttccactt cggcaagggc cgtgggggcg acgtcgccct gctcaaccgg ctcggccccg
     1501 acctcggctt cgacgtccac ggcatggcgc tggtcgacgt cgacggccgg cccaccgccg
     1561 acgaccgccg cgtgtcctcc accgccatcc gccgcgccct ggtcgctggg gaggtcgagg
     1621 ccgctgccgg catgctcggc cgcccccacg aggtgcgcgg cgtggtccac cagggcgacc
     1681 aacggggccg cgacctcggc ttcccgaccg ccaacgtggc cgtgcccggc tcgatcctgc
     1741 tgctggccga cggcatctac gccggctggt tcgtgcgggc cgacggccag gtgctcccca
     1801 ccgccatccg ccgcgccctg gtcgccgggg aggtcgaggc cgccgccgac atgctcggcc
     1861 gcccccacga ggtgcgcggc gtggtccacc agggcgacca gcgaggccgc gacctcggct
     1921 tcccgaccgc caacgtggcc gtgcccggct cgatcctgct gccggccgac ggcatctacg
     1981 ccggctggtt cgtgcgggcc gacggccagg tgctccccac cgccatctcc ctcggtcgcc
     2041 ggcccacgtt ctacgagacg gccgacgcca gcctgctcga ggcccacgtg ctcgacttct
     2101 ccggcgacct ctacgacgag cgcgtcgccg tgcgcttcgt gacccggctg cggggcgagg
     2161 agcgcttcga caccgtcgag gacctcatcg agcagatgca acgggactgc gaagacgccc
     2221 ggcggatcct ggcctcatga ccgggaccgg cttccccgac gacgtgacga tcatcccggt
     2281 caccgtcggg cacgtcgacc ccctcgagcc ggtctacgac gacgacgaga gccgcgagga
     2341 ccgcagccac ctcctaccgc gcgagcgagc ccggcgggcg ctgtccagcg tgctccccga
     2401 ggccgagatg cccgtggtcg ggccggagtc cgccggcgca gccgagcggg gcctcgtcgc
     2461 cgccccccgc ctcgcccgcc acgtgttccg cctgtccgac ggccacgaag tcggcgtcac
     2521 ggtgtccggc cggggcatcc ccctggtgat ggtgcacggc ttcaccgccg aggggttcct
     2581 gtacgcccag acgctctcgc gcctggtgtc gatgggcttc aaggtcgtgg ccatcgacac
     2641 cgccaaccac ggcagcaccc agggcctgcc ctccggcggc gccaacttcc gcgagtacgc
     2701 cacgctgctc gggcgggcgc tcgacgagct cggcatcgag caggcgttct tcgtcgggca
     2761 ctccatgggc ggccgggtca tcacccagct gtgcgccgcc gaacccgagc gggccctcgg
     2821 catcatcctc atcgacgcca tcgtcggcga cgcctgggac cgcctcgtca acgtcggccg
     2881 cctcttcccg ccggccatgg ccggggtcgt ggtcctcctc gccctcgatg ccatgaccac
     2941 cccgcccgtg ctgaagaacc ccgagcaggc cctgaagctc ggtcggctgc tggcgcccac
     3001 cctggtcggc cacgccgtgc ggccctggcg cctgctcggc ccggcggtgt cgatcctgcg
     3061 cagccggggc agccgctgga tgctgcagcg cctcgcccag gagcagatcc cggtcgtggc
     3121 cgtgcacggc acccgggact tcgccgtgcc gctgcagacg gccaaggacg ccgcccgcca
     3181 ggccaggggc gagttcgtgg tcatcgacgg cgccaaccac tgctggcccc tgcgcgaccc
     3241 cgagacgctg cccgccatcg tgtcggacct gctgcacggc agcttcggcg aggcctaccg
     3301 ccaggcggtg cgggaccacg gcctcgagcc cgacctcgcc cgcctcgacg aggtcgagcg
     3361 cgccttctac ccgccagatg ccccgatccg ggacatgacg cccaagctcg agttcgcccg
     3421 gctgacggcc cgccggcgcc ggcccgccta ctcctacgac atcaagcccg ccctgcccca
     3481 gtagtcgggg tgtcgagtct gcaagtcgca tcctcgtgca actgcagtgg tagggtggcc
     3541 gaaggtctcg tgccgtcgcg cctgggggtt ccacccccac ttgtgacgca cggcgccacc
     3601 gcttcccctc ctcgcacagc cggttggggc agccgggtcc catacccacg caagcaacgg
     3661 agtccttcac gtatgtctga gcagatcgac cgcctcaagc cggagctcat cgccgagcac
     3721 cgcctccacg agtcggacac gggttcgccc gaggtccaga tcgcgattct cacgaagcgc
     3781 atcaaccacc tcaacgacca cctctcgacc cacaagaagg accaccacac ccgacgcggc
     3841 ctgctcaagc tggtcggtcg ccgccggcgg ctcctcgact acgtccgcaa gaacgacgtc
     3901 gagcgctacc gggcgatcat cgccacgcac ggcctgcgcc gctgaggggt ccgaacaccc
     3961 cggtggcagc ctcggtcagc tgccagtcgc cgatctccga accacttcgg gggtgcgccg
     4021 actgggaact ggccctgcca ccacgatccg gcccggatct gccacacaca gcccgagggg
     4081 gcgtggcgat caccgggcca caggagacca acccaacatg accaccgaac ccacgcgggt
     4141 gtccgcgccc attcccggcg ccgacaagga gctcgtcttc gagacgggca agctcgcaca
     4201 gcagagccag ggcgcagtcc tggccagcat cgggggcacc aagaccctcg tcaccgccaa
     4261 cgccgccaag agcgtccggg aggggatgga cttcttcccc ctcaccgtcg acgtggaaga
     4321 gcgcatgtac gccgcgggtc gcatcccggg ctcgttcttc cgccgtgagg gccgtcccac
     4381 cgacctggcc atcctcacct gccggctcac cgaccgtccg ctgcgcccca gcttccccgc
     4441 cgggtaccgc aacgagaccc agatcgtgtc cacgatcatg ggctccgacg gccgcaaccc
     4501 ctacgacgtg ctcaccatca acggcgcctc cgctgcgctg atgctgagcg gcctgccctt
     4561 cgacggcccc atcggcgccg tccgcatggc cttcgacatc gacggcgagt gggtcccgca
     4621 ccccgagcac gacgagatcg aggagtccac cttcgacctc gtggtcgccg gccgggaggc
     4681 cgaggacggc tccatcgcca tcatgatggt ggaggccaac ggcaccgaga aggcctggca
     4741 gcactacgag gccggcgccc ccttcgtcac cgaagaggcc atcgcccagg gcctggaaga
     4801 gtccaagaag tccatcaagg actccatcga cgcccagcgc aagctggtcg aggagtacgg
     4861 cgcacgcccg cccctcgagt ggagcgccgc tgacgactac caggacgacg tgctcgagaa
     4921 ggtccgcgag gtcggcaccg accgcctccg cgaggtcggc tccatcgcca tcaaggccga
     4981 ccgcgaggcc gccgagcgtg ccgtcaaggc cgagatccgc gacgagctgt ccgaggagtt
     5041 cgccggtcgt gacaaggaga tcggcaacgc catcaagacg gtgctcaagg acgtcatccg
     5101 ctcccgcatc gtgaacgagg gcgtccgcat cgacgggcgt ggcccggcgg acctccgccc
     5161 ggtcaccgcc gaggtcggca tcctcgacac cgcgcacggc tccggcctgt tccagcgtgg
     5221 cgagacccag gtcctcaacg tcctgacgct cggcatgccg aagatggacc agcttctcga
     5281 caacctgggt ccccaggaga agaagcggta catgcaccac tacaacatgc cgccgcacgc
     5341 caacggcgag accggtcgcg tgggcagccc caagcgccgc gaggtgggcc acggcctgct
     5401 cgccgagcgc gccctgctgc cggtcatccc gaccatggac gagtggccct acacgttccg
     5461 cctcgtctcc gaggtgctcg cctccaacgg ctccacctcg atggcgtcgg tctgctcgtc
     5521 gtcgctgtcc ctgatggacg gtggcgtgcc gatcaaggcc gccgtcgccg gcatcgccat
     5581 gggcctcgtg tacgccgacg gcaagtacac gaccctcacc gacatcctcg gtgccgagga
     5641 cgccttcggc gacatggact tcaaggtcgc cggcacgcgg gacttcatca ccgccctgca
     5701 gctcgacacc aagatcgacg gcctccccgc cgacgtcctg gccgacgcgc tggcccaggc
     5761 ccgggatgcc cgcaacacga tcctcgacat catggacgag tgcatcgcgg agccccgtga
     5821 cgacgtccag ggctcggccc cgaagatcat cagcttcgag atccccgtcg acaagatcgg
     5881 cgaggtcatc gggcccaagg gcaaggtcat caaccagatc cagggcgaga ccggcgccga
     5941 catcggcgtc gacgacgacg gcgtcgtcgg tgtggtcacc atcggtggca ccgacaaggg
     6001 tgccgtcgaa gaggctcgcc gccagatcga gctgatcgtc aacccgccct ccgcccaccc
     6061 gggcgagatc tacatgggca aggtcgtcaa catcaccaag ttcggcgcct tcgtcaacat
     6121 cctccccggc cgcgacggcc tgctccacat ctccaagatc ggcggcaagc gccgcatcga
     6181 caaggtcgag gacgagctgg agctgggtca ggagatcgag gtccgggtcg acgacatcga
     6241 tccgaacggc aagatcagcc tgtccccggc cagcgagccg gccgacgagg gtggcgacga
     6301 cgacggtccc aagcgcgagc gcagcgaccg tggtggccgc gacggtggcc ggggtcgcag
     6361 cagcgaccgc ggcgatcgcg gtggcagcaa ggacagcaag gaagacaccg gcggcaccgc
     6421 tgccggtggc accgcctcct ccggcgagtt cgtgtccttc gaggacgcgt tcgacgagga
     6481 gctgaaggag gagttcggtg acctcggtcc cgagggttcc tccggtggtg gcgacggtgg
     6541 caaccgtggc ggtggccgcg gtcgtggccg cagcggcggc ggcggtcggc gccgctgagc
     6601 gtttctgagg gcggtgcggg gctcgatcct gagccccgca ccacccccct ggccaacggc
     6661 gtccgcgtcg tcagcgacgt catgccccac gcccggtcgg tcacgaccgg cgtgtgggtg
     6721 gccgtcggcg gccgtgacga gccggccgag ctggccggcg cgtcgcactt cctcgagcac
     6781 ctcttcttca aggggaccga ggcccgctcg gcgcggtcca tcgccgaagc cgtcgacgcg
     6841 gtcggcggcg acatgaacgc gttcacggcg cgggagcaca cgacgttcgt gacccggctc
     6901 ccggctgcgc actggcggct cgggatggag ctgatcggtg acatcctgtc cgcgcccgcg
     6961 ctgcgacccc acgagatcga cgccgagcgc gaggtcatcc tcgaagagct gtgggctgcg
     7021 ctcgacaccc ccgaggaccg cgtccacacg ctgtccatgg aggcgctgtt ccccggccat
     7081 gccctcggcc gcgaggtgct gggggacgag gacaccgtgc gggccatgac ccgcgacgac
     7141 atcctcgggt tcttcgagac ccactaccgc ccggccaacc tcgtcgtcgc ggcggccggc
     7201 aaggtcgacc acgacgagct gtgcgaggtc gtctccgagc gcctcggcgg cggcgacggc
     7261 ggcgccgaac cggcccgcca cgcgccgacc ccggacgtcg acgatctgcg cacccaggtg
     7321 cgcgacggcg agcaggtgca cgtcatcctc ggctggcgcg gcctgcccct cgaccacgag
     7381 gaccgctacg ccctccacgt cgccgaccag gtcctgggcg gcggttcgtc gtcacgcctg
     7441 ttccaggcga tccgcgagga gcgcggcctg gcctactcgg tgtactcctg ggtgtcgtcg
     7501 tactccgacg ccgggctgct cggcgtctac gccggcacct cgccgggccg gtcaccccag
     7561 ctgctcgacg ccctcgaatc cgagatcgac acgatccgaa ccggcggcgt caccgccgac
     7621 gaggtccggg tcgccaccgg ctacctggcc ggcgccaccg agctcggcct cgaggactcc
     7681 ggctcccgga tgacccgcct cggtcgcatg ctgctgtcga ccggctcggt cgtgagcatc
     7741 gacgagcagc tcgagcgcct ccacgccgtc accgtcgacg atgtcaacgc cgtgctccag
     7801 caggtgctca gcgcaccccg ctcggtcgcg gcggtcggcc cggtcgacac cacgaggctc
     7861 gctcgcgccg tctgaagccc gccgcgtggc cacggcagga cggtcgacgg ccacgacggg
     7921 cggggcgagg gcggcagcgg gctggcatgg agcggacggt accgacggcc acggcgcccg
     7981 tcccgctgtt gacgccgcgt caacgtgctc gggttgccta ctcttccgaa catgacgatt
     8041 cgggtgggtg tcttcggagc cggcgggcgc atgggcagca cggtgtgtgc tgctgtcgct
     8101 gcggatcgtg agtgcgagct ggtcgcggcc gtcgacccgc accacgaggg catcgacctg
     8161 cacctggtcg cccgaggcac cgacctcccc ccgctcaccg tcgccggcgg tgccgaggag
     8221 ttcgagcgcg ccgaggtcga ggtcgtggtc gacttcaccc acctcgaggc cgcgtgcgac
     8281 aacctggcgt gggccgccga gcacggggtg cacgccgtcg tgggcaccac cgggttctcc
     8341 gccgccgacc tcgcccgctt cgagtcgacc ttcaccgact ccaactgcat gatcgtgccg
     8401 aacttcgcca tcggcgccgt gctcatgatg cgcttcgccg agatggcctc gccgttcttc
     8461 cagaccgccg aggtgctcga gtaccaccac gacgagaagg tcgacgcccc ctccggcacc
     8521 gccatgctga ccgtcgagcg gatggccgcc gcgtccgagg agtgggctga ggaccccacg
     8581 cgcaaggagg tcgtggccca cgcccgcggc ggtgagggcc ccggcggcat ccacgtccac
     8641 tcggtgcgca tgcggggcat ggtcgcccac caggaggtcc tgctcggcac cgagggccag
     8701 accctctcga tccgccacga ctcctacgac cgctcgtcgt tcatgcccgg cgtgctgctg
     8761 gccgtgaagg cgatcggcga ccgccccggt ctcaccatcg gcctcgacga ggtcctcggc
     8821 ctctagagga ttcgttctgg cctacatccg tgccacgtgg tggtcatgca tgtaggccag
     8881 aacggctcag ggctccgtcg gctcggggac ggcggccttg tcgggacgct cggcgggcac
     8941 gccgttcagc tcggcccagt cgaggccgaa ctgcatccgc tcggccacgc cggggtggct
     9001 ggcgttgagc ttgtcccacc agcccggctc caggtcggcc ttgttgagcg ccacgaacga
     9061 gggccacatg gccacgaagc tggtcgggtc accgagcagc tcgagggcct cgaggtcggc
     9121 ctcccgctcg ttgcgccggg acacccagga cgagaccagc ccgagcgccg ccatgggcag
     9181 gccgagtccc agcaggaaca gcggcgcggc gcccgggtcg ccgaggtcgt cgacgccggc
     9241 ccagccgagg acggtgtcgc tgccgatcac gtactgcacg aacgcgagcg tgatcagcat
     9301 ctgcagcccg gccagcggga acgtcttggg gatgtggttc agccggtagt ggccgatctc
     9361 gtgggccacc acctgggcga tctgctcgag cgggaactcc atcatcgtgt cgtagaccac
     9421 cacccgcttc gtcggtccga agccggcgac gaacgcgttg ccccggcggg atcgcttcga
     9481 gccgtccatc gtgtagacgc cctcgatgct ggtgtcggcc agcacggcga tctcctcgat
     9541 gcgggtgcgc aggggacccg gctccatcgg ggtgaacttg ttgaacctcg gcatgatcag
     9601 caccgggtag acgaagttca ggaacagggc gatgccgagg aacagcaggc cgcccaccag
     9661 ccaccacgtg tcgaacgtgc ggatggccca gaagatcggg accagcagca gggcggagag
     9721 cacgacgttg ag
//
LOCUS       DATISU010000008         4396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_279871, whole genome shotgun sequence.
ACCESSION   DATISU010000008 DATISU010000000
VERSION     DATISU010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279871"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..753
                     /locus_tag="VLR27_00470"
     CDS             175..753
                     /locus_tag="VLR27_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HSP01942.1"
                     /translation="MTANPDVDQYLERSETWPDEVAALRPVLLGCGLTEQLKWSKPCY
                     SHDGNNIVIVQEMKDFLALMFFKGALLRDPDGVLREQGPNSRSARRMELTSVDEVRAS
                     AGTIEAYVAEAVAVEEAGLEVPAAEVELVDELQGRLDEDPGLKEAFESLTPGRQREYN
                     LHIGAAKQSKTRVARVDRCVERILSGRGLRDR"
     gene            complement(769..1512)
                     /locus_tag="VLR27_00475"
     CDS             complement(769..1512)
                     /locus_tag="VLR27_00475"
                     /inference="COORDINATES: protein motif:HMM:NF018755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HSP01943.1"
                     /translation="MPFRTPLSVRHDIGEEWCLLEPLIYEGNRDFVVVRQGFRTDFAS
                     IPRPVRWLFDTTGTNSEPGVLHDALWRESKRDDGRTPRVDAWDADGLFRRALRESGAT
                     ALARGLMWIAVRAAAIAAGRFGRSGPPWPVKLSKMAGMATVGLVTVGAPTAVAFVGRI
                     FYWVVEWVVAAPWSVVERLRGREPNLPWPLGVRHEQREEPPREYLLLIPVDSPGGQAL
                     ATMLGPEGDTVIHDEQLDRLPSETLESAY"
     gene            1764..2210
                     /locus_tag="VLR27_00480"
     CDS             1764..2210
                     /locus_tag="VLR27_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSP01944.1"
                     /translation="MVSIRGEFDLATRAYLSDTIDRAARLDDGDVVVDLSGVTFMDAS
                     TIGAIVDAHNRLRARARSLSVRAPLPRARRLFDVCDLGFLIEDPPERPQAGAALKSWV
                     AVPASDRAPDAGRPRSTPTPPGAPARTWAEGAVERAGATLRHRAPS"
     gene            2207..2938
                     /locus_tag="VLR27_00485"
     CDS             2207..2938
                     /locus_tag="VLR27_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HSP01945.1"
                     /translation="MTDGGHDRRLAILDRLLGHQEGFATERLCQVCADVTGASGAGIM
                     LMAGDIPHGSVCTTDDVSALIEQLQYDLGEGPCVDAYEKDRPVLEPDLAHPTVPRWVA
                     FAGPAVDAGARAVFGFPLQVGAVRLGALNLYNDEPGSLTDDQYNDALVMADVAAQAVL
                     LLQADAPPESVAAELESNANFQYVVHQASGMVAAQLEISVGLALIRLRAHAFGSGGSL
                     TGVARAVVDRKLRFDADGLAEDRKP"
     gene            3042..3779
                     /locus_tag="VLR27_00490"
     CDS             3042..3779
                     /locus_tag="VLR27_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HSP01946.1"
                     /translation="MTRESVLARTLVELADTLVDDFDVVDLLTLLADRCVEVLDVDAA
                     GIMLAAPDGQLRVMASSSEAMRVLELFEIQAQEGPCLECHRTGRPVVNQDLDAGDGRW
                     PRFAAEALDSGFRAAHALPMRLRGSVIGALNLFRADVGEMGPADIEVAQAFADVATIA
                     ILQHRAALEAHVLSQQLTHALNSRVMLEQAKGMVAERLTLDMEQSFSALRNHARSNNL
                     RLSDVAQSVIEGSLAPSDLQAAAAGNR"
     gene            complement(3789..4379)
                     /gene="def"
                     /locus_tag="VLR27_00495"
     CDS             complement(3789..4379)
                     /gene="def"
                     /locus_tag="VLR27_00495"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HSP01947.1"
                     /translation="MPFRLPGRCPPITLYGNPVLRTHAQPVTAFDRDLASLVDDLFDT
                     MYAIETGVGLAANQIGRLERVFVFDCGEDETGYVVNPIVEVIGDGAQTGAEGCLSVPG
                     VSVPTTRGARARVQGLDVRGEPVTYEGEGLVARCFQHEVDHLDGMLYVDRHPDLLPAI
                     DRHLQDSDWFGTAALDPRSAQYRRAQAADRPFDPVV"
BASE COUNT          650 a         1612 c         1512 g          622 t
ORIGIN      
        1 gcgaagtcgt agctgcggat gatcaggtcg gcggagtggg tcccgccgag ccgctcccgc
       61 accccctcgt tgatcagccg ctcgtactcg atcgagctct cccagctcat tccgccgagc
      121 aggccgatgc aacgcatcag cagaacgtag acggaccgac ctactgtcac cggggtgacg
      181 gcgaaccccg acgtcgatca gtacctcgag cgctccgaga cgtggccgga cgaggtcgcc
      241 gcgctgcgtc ccgtcctgct cgggtgtggg ttgaccgagc agctcaagtg gtccaagccc
      301 tgctacagcc acgacgggaa caacatcgtc atcgtccagg agatgaagga cttcctggcg
      361 ctcatgttct tcaagggagc gctgctccgc gaccccgacg gggtgctgcg cgagcagggt
      421 ccgaactcgc gatctgcccg gcggatggag ctcacgtccg tggacgaggt ccgcgcgtcg
      481 gccgggacga tcgaggccta cgtcgccgag gcggtcgccg tcgaggaggc cggcctggag
      541 gtgccggcgg ccgaggtcga gctcgtcgac gagctgcagg gtcgcctcga cgaggacccc
      601 gggctgaagg aggcgttcga gtcgctgacg cccggccggc agcgtgagta caacctccac
      661 atcggcgcgg ccaagcagtc gaagacccgc gtcgcccggg tcgacaggtg cgtcgagcgg
      721 atcctgtccg ggcggggcct gcgcgaccgc tgacgcaggc ggcagagctc agtacgccga
      781 ctcgagggtc tcgctcggca accggtccag ctgctcgtcg tggatgaccg tgtcgccctc
      841 cggcccgagc atcgtggcca gcgcctgccc gccgggtgag tccaccggga tgaggagcag
      901 gtactccctc ggcggctcct cgcgctgctc gtgccgcaca ccgagcggcc atgggaggtt
      961 cggctcccgg ccgcggagcc gctcgaccac cgaccagggc gcggcgacga cccactccac
     1021 gacccagtag aagatgcgcc ccacgaacgc caccgcggtc ggtgcaccga cggtgaccag
     1081 gccgacggtc gccatgccgg ccatcttcga cagcttcacc ggccacggcg gcccgctgcg
     1141 gccgaaccgg ccggcggcga tcgcggcggc gcggacggcg atccacatga gcccgcgggc
     1201 gagtgccgtc gcccccgact cgcgcagcgc tcggcggaac aggccgtcgg cgtcccacgc
     1261 gtcgactcgt ggcgtccggc cgtcgtcccg cttcgactcc cgccagagcg cgtcgtggag
     1321 gacgcccggc tcgctgttgg tgccggtcgt gtcgaacagc cagcgcaccg gccgggggat
     1381 cgaggcgaag tcggtccgga agccctggcg cacgaccacg aagtcccggt tgccctcgta
     1441 gatgagcggt tcgaggaggc accactcctc accgatgtcg tgtcggacgc tcagcggggt
     1501 gcggaagggc acgcccctga cggtagatgg atgccccgca cgagaccggg gaccgaggtg
     1561 tacggtggac tcgacacgcc gaacgaagcc ctgacgtgtc gggccagcgc acatgctggt
     1621 ccgggagcgg tactccggac cccggtagcg cgcgttgctc gcaacgaccg aagccaggag
     1681 ctgtcatggg taccaacgaa cggccgtcag cggcggtgta cccccgcgga tctgccgaac
     1741 cggagagcga tcccctccgc accgtggtct cgatccgagg cgagttcgac ctcgccacgc
     1801 gggcgtacct ctcggacacc atcgatcggg ctgcccgtct cgacgacggc gatgtcgtcg
     1861 tcgacctcag cggcgtcacg ttcatggacg cgtcgacgat cggtgccatc gtcgatgccc
     1921 acaaccggct ccgcgctcgc gctcgttcgc tgtccgtccg tgcgccgctg ccgcgggccc
     1981 ggcgactctt cgacgtgtgc gatctcgggt tcctgatcga ggacccgccg gagcgtccgc
     2041 aggccggcgc cgcgctgaag tcgtgggtcg ccgtacccgc atcggatcgc gctccggatg
     2101 ccgggcgacc gcgctccacg cccacccctc cgggggcacc ggctcgcacc tgggccgaag
     2161 gagccgtcga gcgggcaggg gcgacgctgc gacatcgagc tccgtcgtga ccgacggcgg
     2221 ccacgatcgt cggctggcga tcctcgatcg cctgctgggc caccaggagg ggttcgcgac
     2281 cgagcggctc tgccaggtgt gcgcggatgt caccggtgcc tccggcgccg ggatcatgct
     2341 catggccggc gacatccccc acggttcggt gtgcaccacc gatgacgtga gcgccctcat
     2401 cgagcagctg cagtacgacc tgggcgaagg accgtgcgtc gacgcctacg agaaggaccg
     2461 tccggtgctc gagcccgacc tcgcccaccc gaccgtgccc cggtgggtgg ccttcgccgg
     2521 gccggccgtg gacgccggcg ctcgggccgt gttcgggttc ccgctccagg tcggcgccgt
     2581 ccgcctcggt gccctgaacc tctacaacga cgagcccggg tcgctcaccg atgaccagta
     2641 caacgacgcg ctggtcatgg ccgacgtcgc tgcgcaggcg gtcctcctcc tccaggccga
     2701 cgcacccccc gagtccgttg ctgcggagct ggagtccaac gccaacttcc agtacgtggt
     2761 gcaccaggca tcgggcatgg tcgcggccca gctcgagatc agcgtcggcc tcgccctgat
     2821 ccgcctccgg gcccatgcgt tcggcagcgg tggctccctc accggcgtgg cccgagccgt
     2881 cgtcgaccgg aagctgcgct tcgatgccga tggcctcgca gaggaccgga agccgtgagc
     2941 accgccgccg gacagcgccc gaacccgcca cgccgtacca tctgtcagac accgatcgag
     3001 cgacgagagc cattggcgac ctccgttcga aggagcaccc aatgaccaga gaatccgtcc
     3061 tggctcgaac actcgtcgag ctggccgaca ccctggtcga cgacttcgac gtcgtcgacc
     3121 tcctcaccct gctcgccgac cgctgcgtcg aggtgctcga cgtggacgcg gcggggatca
     3181 tgctcgcggc gcccgacggg cagctccggg tcatggcctc ctccagcgag gccatgcggg
     3241 tcctcgagct gttcgagatc caggctcagg aagggccctg cctcgagtgc caccgcaccg
     3301 gtcggcccgt ggtgaaccag gatctcgacg ccggggacgg ccggtggccc cggttcgcag
     3361 ccgaggcgct cgactccggg ttccgtgctg cgcatgccct tcccatgcgc ctgcggggct
     3421 cggtgatcgg tgcgctcaac ctgttccgcg ccgacgtcgg ggagatgggg cccgcggaca
     3481 tcgaggtggc ccaggccttc gcagacgtcg ccaccatcgc gatcctgcag caccgagccg
     3541 cactcgaggc ccacgtcctg tcccagcagc tgacccacgc cctgaacagc cgggtcatgc
     3601 tcgagcaggc caagggcatg gtcgccgaac ggctcacgct cgacatggag cagtccttct
     3661 cggccctccg caaccacgcc cgcagcaaca acctccggct cagcgacgtc gcgcagtccg
     3721 tcatcgaggg ctcgctcgcc ccctcagacc tccaggccgc cgctgctggc aaccgctgac
     3781 gccggcggtc acaccaccgg gtcgaacggc cggtccgcgg cctgcgcccg ccggtactgc
     3841 gcgctgcggg ggtcgagcgc ggcggtcccg aaccagtcgc tgtcctgcag gtgccgatcg
     3901 atggcgggca gcaggtcggg gtggcggtcg acgtagagca tgccgtcgag gtggtcgacc
     3961 tcgtgctgga agcaccgggc gacgaggccc tcgccctcgt aggtcaccgg ctccccgcgg
     4021 acgtccaggc cctgcacccg cgcccgggca cctcgagtcg tcggcacgga caccccgggg
     4081 accgacaggc agccctcggc gccggtctgc gcgccgtcac cgatgacctc gacgatcggg
     4141 ttcacgacgt agccggtctc gtcctcgccg cagtcgaaga cgaagacccg ctcgagccgg
     4201 ccgatctggt tggcggcgag cccgacgccg gtctcgatgg cgtacatcgt gtcgaacagg
     4261 tcgtccacca gcgaggccag gtcacggtcg aacgccgtca ccggctgggc gtgcgtccgc
     4321 agcacggggt tcccgtagag cgtgatgggc gggcacctgc caggcagtcg gaacggcacc
     4381 cggtgacgct atcccg
//
LOCUS       DATISU010000009         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_559549, whole genome shotgun sequence.
ACCESSION   DATISU010000009 DATISU010000000
VERSION     DATISU010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559549"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VLR27_00500"
     CDS             complement(<1..380)
                     /locus_tag="VLR27_00500"
                     /inference="COORDINATES: protein motif:HMM:NF017697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate hydrolase family protein"
                     /protein_id="HSP01948.1"
                     /translation="MAYHGGDLEVGTDQIADVVAERTGASLYAVRQPEGMRHHLPSKR
                     FRPEHSERLAAFVDHVDVVITLHGYGRRSMFRTVLLGGGNRGLAGVVAASLRRTLPGY
                     EVIDDLDDIPRELRGVHPHNPVNLP"
     gene            complement(452..1309)
                     /locus_tag="VLR27_00505"
     CDS             complement(452..1309)
                     /locus_tag="VLR27_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HSP01949.1"
                     /translation="MIDAPLALALASGMVAAVNPCGFAMLPAYVTFFLGHEGDRVPSR
                     GESVARAIPVALAVSLGFVVVFGVVGIALRPISSTVQEYAPWATIVIGVGLAILGVAM
                     VAGKELKVRVPVLDRGGRSGGLGSMFLYGVSYAIASLTCTIGIFIANIVNAFSRTDFV
                     SGVAVLVTYAAGMGLVITALTVAIALARDSVVRVLRSGMRHANRAAAVLMVVMGLYVA
                     WYGVFSLRVRSDPGTRAGPVDRVEQWSADATRFVSDVGATRLGIYLLAAIAAMVSISV
                     LVTSLRRQP"
     gene            complement(1306..1911)
                     /locus_tag="VLR27_00510"
     CDS             complement(1306..1911)
                     /locus_tag="VLR27_00510"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSP01950.1"
                     /translation="MTPLSRSRLPWQAVVAATVLAVLAALAVVAVLGGGDPDDGVTTG
                     SDSTPSDTLQLTPAEDAATGEPLDIPVAWPDGAEVALAEVIEGPTVVNFFASWCPPCI
                     AEMPDFESVSQEAGDDVEFIGVAVQDRTADATRIVEDTGVTYRWARDARGDVANAAQV
                     TQMPSTMFVDADGEVVSLHAGALDADQLRDLLESELGVTVG"
     gene            complement(2076..2471)
                     /locus_tag="VLR27_00515"
     CDS             complement(2076..2471)
                     /locus_tag="VLR27_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HSP01951.1"
                     /translation="MLAQPEQLVEISPRDNVLDVALEVDTSFWSEAACAVDGGIQAHL
                     FFSEELLDIAAAKRICAECPVLERCLEGALARKEPLGVWGGQLFSNGKVLASKRRRGR
                     PPKNPRPEDEFVHVEVPAALQDLVELRTA"
BASE COUNT          412 a          890 c         1051 g          336 t
ORIGIN      
        1 gggaggttca cggggttgtg ggggtgcacg ccgcgcagct cccgcgggat gtcgtcgagg
       61 tcgtcgatga cctcgtagcc cggcagggtc cgtcgcagcg acgcggccac cacgccggcg
      121 aggccccggt tgccgccgcc cagcagcacg gtgcggaaca tcgaccgccg gccgtagccg
      181 tgcagcgtga tcacgacgtc gacgtggtcc acgaaggcgg cgaggcgctc cgagtgctcg
      241 ggccggaacc gcttggacgg caggtggtgg cgcatgccct cgggctggcg cacggcgtag
      301 agcgaggcac cggtgcgctc ggccaccacg tcggcgatct ggtcggtgcc gacctcgaga
      361 tcgcccccgt ggtacgccat cacgccgacg cgcgagccga gcgccaggtc ctcgacgacg
      421 ccgtcggccg ccagcagcga gcggaggtcg ttcagggctg tcgccgcagg ctggtgacca
      481 gaaccgagat cgacaccatg gcggcgatgg cggcgagcag gtagatgccg aggcgggtgg
      541 cgccgacgtc ggagacgaag cgggtggcgt cggccgacca ctgctcgacc cggtcgaccg
      601 ggccggcccg ggtgccgggg tcgctgcgca cccgcagcga gaagacgccg taccaggcca
      661 cgtacagccc catgaccacc atcagcaccg cggcagcccg gttggcgtgg cgcatccccg
      721 agcgcaggac gcggaccacc gagtcgcggg ccagggcgat ggcgacggtg agggcggtga
      781 tgaccagccc catgccggcg gcgtaggtga cgagcacggc caccccggag acgaagtcgg
      841 tgcggctgaa ggcgttgacg atgttggcga tgaagatgcc gatggtgcag gtgagcgagg
      901 cgatggcgta ggagacgccg tagaggaaca tcgacccgag cccgcccgag cgcccgccgc
      961 ggtcgagcac cggcacccgg accttcagct ccttgccggc gaccatcgcc acgccgagga
     1021 tggccagccc gacgccgatg acgatggtgg cccacggcgc gtactcctgg accgtggagc
     1081 tgatcgggcg cagcgcgatg ccgacgacgc cgaagaccac gacgaagccg agcgacacgg
     1141 cgagcgccac cgggatggcc cgggccacgg actcgccccg gctggggacc cgatcgccct
     1201 cgtggccgag gaagaacgtg acgtaggccg ggagcatggc gaacccgcac gggttcacgg
     1261 ccgccaccat gccggaggcc agcgcgaggg cgagcggggc gtcgatcaac ccaccgtcac
     1321 cccgagctcg gactcgagca ggtcacggag ctggtcggcg tcgagggcgc cggcgtgcag
     1381 ggagacgacc tccccgtcgg cgtcgacgaa catggtcgac ggcatctggg tgacctgggc
     1441 cgcgttggcg acgtcgccgc gcgcgtcgcg ggcccagcgg taggtgacgc cggtgtcctc
     1501 gacgatccgc gtcgcgtcgg cggtgcggtc ctggacggcg acgccgatga actcgacgtc
     1561 atcgccggcc tcctgggaca cggactcgaa gtccggcatc tccgcgatgc acggcgggca
     1621 ccaggaggcg aagaagttga cgaccgtggg accctcgatc acctcggcca gcgcgacctc
     1681 ggcgccgtcg ggccaggcga cggggatgtc gagcggttcg ccggtggccg cgtcctcggc
     1741 cggggtgagc tgcagggtgt ccgacggggt gctgtcggac ccggtggtga cgccgtcgtc
     1801 ggggtcaccg ccgccgagga cggcgacgac cgccagtgcg gccagcacgg cgaggacggt
     1861 ggcggccacc acggcctgcc acgggaggcg agagcgagag agcggggtca tggcgggcaa
     1921 gccgcgacgg tactgccgcc ctcgtcgccg gtcacagcgg tgatgctcgc cgtgaccggc
     1981 ggggggaggt tgggccgcgg gcgggccgga gctcgatgcg ccgttgctcc ggcccgccgt
     2041 ccctgcggcg gggggttcga tcgattgcgc tcgacctaag cggtgcggag ctccacgaga
     2101 tcctggagcg ccgcggggac ctcgacgtgg acgaactcgt cctcggggcg agggttcttg
     2161 ggcggccgac cccggcgccg cttggacgcc aggaccttgc cgttgctgaa gagctggccg
     2221 ccccagaccc cgaggggctc cttgcgggcg agggcgccct cgaggcaccg ctcgagcacg
     2281 gggcactcgg cgcagatgcg cttggcggcg gcgatgtcca gcagctcctc ggagaagaag
     2341 agatgggcct ggatcccacc gtcgaccgca catgcggcct cggaccagaa cgaggtgtcg
     2401 acctcgaggg ccacgtccag gacgttgtcc cggggactga tctcgacgag ctgttcgggt
     2461 tgagcgagca tcgcccgctc ccttctcttg ctgggtggat gttgtcggtg ggtgggtggt
     2521 gaggcagggc cggaacgtgg cccgagaaag cgaaaggccg tcgggatgtt cccgacggcc
     2581 tctgcggtcc ttggtgcctg accagtggtc aggtggtgga acacagaggc cttcgatcgt
     2641 ggaacgccgg cacggtcatg gagaggcgcg aggtggcgag ccaggaacc
//
LOCUS       DATISU010000010        27097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_326534, whole genome shotgun sequence.
ACCESSION   DATISU010000010 DATISU010000000
VERSION     DATISU010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 27097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27097
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326534"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VLR27_00520"
     CDS             <1..555
                     /locus_tag="VLR27_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HSP01952.1"
                     /translation="WQRFANLRTLYGWMWGHPGKQLLFMGCEFAQEREWSESRSLDWH
                     LLDDPRHAGVQQLISALNQVHAEHPAMWRRDTEPAGFDWLDASDAGQSVLAFQRNGRP
                     DEDDPVVCVANFTPVPRHGYRIGLPRAGAWREVLNTDDERFGGAGIVNSAIESEDVPW
                     QGREQSAVVTLPPLGVAWFAPESP"
     gene            793..1635
                     /locus_tag="VLR27_00525"
     CDS             793..1635
                     /locus_tag="VLR27_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HSP01953.1"
                     /translation="MTTEVQTCYRHSDRRAGVRCQRCERPICPSCMNQASVGFHCPEC
                     VKGAGQKVFTARTMPSSANPIVTTTLVAMNVAIYVASSLGAGLSEPSFQVQLDYALIG
                     RGLSSEGLLGVAEGEWYRLISGGFLHGSLVHLGFNMFVLWVLGKQLEPALGRVGFAAV
                     YFASMLAGSFGVMLLDPGALTVGASGAVFGLFGYAVVAQFVRGINPLQTGLGAVILLN
                     LAFTFLVPGISIGGHVGGLVGGLVAGALHDLVRPKLGAPPQVGVALVTAFGVACAVGS
                     VLVL"
     gene            complement(1632..2216)
                     /locus_tag="VLR27_00530"
     CDS             complement(1632..2216)
                     /locus_tag="VLR27_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP01954.1"
                     /translation="MADAVSTPIPDQRSAILSAALAVLARDGEAAFTVRRIAEEAGCS
                     TTGVYTWFGGKAGLVDAIFIDGFESFDAALAPAYASGDVGASGRAYRRWALENPTQFL
                     VMFGRAVPDVVPGDAALERGYESFLRLVHHVHAIRPDLSEGDAFDWAYHVNATVHGYV
                     LTEITGMSTAPDRAEELYELGLRRLLQPLVGERG"
     gene            2271..3671
                     /locus_tag="VLR27_00535"
     CDS             2271..3671
                     /locus_tag="VLR27_00535"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HSP01955.1"
                     /translation="MTATEQPTDQSGYGNRYLTGPYAPVTEEVTAFDLPIEGELPEEL
                     AGRYLRNGPNPIDDVDPATHHWFLGDGMVHGVRLLDGRAEWYRNRYVGSQHVGEVRGT
                     GDVPGPNWNDSPGGPNTNVGGFAGTTWAMVEAGGTPVELTYELETVGRNDFSGTLPGA
                     FTAHPKLDPDTGEMHAMVYAPANWMDHIQYVVVAPDGRVRKALDIPLGMTMLHDMSLT
                     QRYAVIYDQPCTVDIELAMAGDSFPMRWNPDYGNRLGLLPREGAAEDIIWIDVPIGYV
                     FHPMNAFDDADGKVVIDLCRYDKMFDVDVLGPFGDGGHATLERWELDPVARTVSTTVV
                     DERANEFPRHRGSLTAKPYRYGYCASPSLDDGAGWPTLKHDLQTGQRWEFDHGAGRAA
                     GEPVFVARQGGTDEDDGWLLTFVHDLGSGDTDFVVMDAADLDRRDYVARVRLPQRVPL
                     GFHGNWVSDRAVPPPM"
     gene            3704..5425
                     /locus_tag="VLR27_00540"
     CDS             3704..5425
                     /locus_tag="VLR27_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP01956.1"
                     /translation="MTDHYREAWDELTAPGGSFEIETIDVRGVPIQVFKSAPPNMRVL
                     WELSAGHGDKDYVVYEDERYTYAEVHAQVRALAHLLRETHGVREGDRVAIAMRNYPEW
                     VVSYWATLSLGAAAVGMNAWWTGPEMEYGLTDSRPKVLIADDERLERVLPHLDAVRAE
                     QPMHVITVRSDRELPDDAARWTDVVRAEDAPDALPEATIDPDDDACIFYTSGTTGFPK
                     GAQLTHRGCVHNILHIVFMTTAAGMAAQKAGTAPAPDPDAAPSQAVFMAPTPLFHVTA
                     NNCLLHPCTLAGGRIVFTYKWDPGRALELIERERVTNFSGVPTMSRELLAHPDWEKRD
                     TSSVQGMGGGGAALQPDLVDKIDKSLKSGAPSTGYGLTETCGIVTANSAGNYVAKPAS
                     CGPVVPTLEAKLVDEAGNDLPPGPDTVGQLCVRGSVVIKGYLNRPEATAEAIQDGWFN
                     TGDIARIDEDGFVFIVDRAKDMVIRGGENIYCSEVESAIYEHDDVAEVAVFGVPDERL
                     GEEVGAAIVLRNGASLDEEGLRSFLSERVAKFKIPAHVWFRTEPLPRNANGKFVKRDL
                     RDEYVGA"
     gene            complement(5422..6531)
                     /locus_tag="VLR27_00545"
     CDS             complement(5422..6531)
                     /locus_tag="VLR27_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HSP01957.1"
                     /translation="MTTFLLRLDPDEGGGPTVAVKDLIDIAGTPTTCASRPVAEAARP
                     ATADAACITSIRAAGGRIVGKVNLHELAFGGSGINPYTGTPQNPLDPTRIPGGSSSGS
                     AVAVATGEADIAIGTDTAGSVRIPSTCCGVVGLKTTFGRIPTAGVRPLAPSLDTVGPM
                     ATTVAGVVAGMALLEAGFAPGTAAPSLGRFRLPGAHPEIEAAIDRLLDASGLHIAPTA
                     LPGWEEAADAGTAILFGEAARVHASLVRDHADELGDETRDRLAKGAGITEGALDAARA
                     HAEPWRAELDAALARTPVIATAGIMDEPPLLTEPQRLDTRAPNVAVNLAGNPAIVVPV
                     ARPGHLPTAVQLIGRRGEEEVLVATAAVLEAASAA"
     gene            complement(6528..6989)
                     /locus_tag="VLR27_00550"
     CDS             complement(6528..6989)
                     /locus_tag="VLR27_00550"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSP01958.1"
                     /translation="MARITVSTTIDASPAAVWAAVEDVGSHVEWMADAEAIRFTSDHT
                     SGVGTTFECDTKVGPLRLTDLMEITRWEPGTAMGVRHVGLVTGEGAFTLAPAGRRLRR
                     RSATRFEWSERLVFPWWMGGPIGGIVGGRIMRVIWKGNLRRLKAIVEGRTA"
     gene            7052..8305
                     /locus_tag="VLR27_00555"
     CDS             7052..8305
                     /locus_tag="VLR27_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HSP01959.1"
                     /translation="MAVLLALLAAVVAAGLAALVVARLTTTQLSARAVEEREAAVAAA
                     VDAVVAQAGHAFVAEREHTLRAAVESATSVATSKLQDSMTAGSRELDLRSRSFEQQVT
                     HIADALGTLGGLVQSLQKERAEQTGSLVAQLEHVTRTQQSLAQHTDQLRQALASPKQR
                     GQWGERMAEDVLRAAGFREGVSYRRQTAIEGGTIPDFRFLLPRDLELRMDVKFPVDNY
                     LRVLECDSDADRARHTKAFVADVRTHVRGLAGRGYVDHRDTVDCLLLFIPNEAVYAFL
                     HESDPDIVDVALGHQVVLCSPSTLFAVLAVVRRSVEQFQLERRSDDILRCLGGLTGEW
                     DKLVGAIEKVGRQLDLTHKSFHDELNGTRRRVFQRSLDEIESLQSATERPVLSAGEAL
                     AAQKAAGDVRALPVDDSDRGVAEAG"
     gene            8413..8658
                     /locus_tag="VLR27_00560"
     CDS             8413..8658
                     /locus_tag="VLR27_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01960.1"
                     /translation="MSDYEIPKPEPKEIDSVKWLEWKDVMERATLKGHPYKDERCDNC
                     LYYLDPDEEISYCWHQTLRILVGGDWWCQWWEPIEEE"
     gene            8661..8852
                     /locus_tag="VLR27_00565"
     CDS             8661..8852
                     /locus_tag="VLR27_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01961.1"
                     /translation="MAGDELPDDVPEADALEQRRAVDDQPDADADAVLDDDSFEVPEA
                     DALDQARAVPTDDEPDPEG"
     gene            complement(8901..9437)
                     /locus_tag="VLR27_00570"
     CDS             complement(8901..9437)
                     /locus_tag="VLR27_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSP01962.1"
                     /translation="MNVITLLEKDHQEVDELFRRVNLSDKPESIDELTKQIVHDLSVH
                     AAVEEQFVYPLLRAKVDGGNAMADEAIDEHQQAKRLLADLEKLDAGSAEHGEAMEALI
                     ETIRHHVEEEESDVLPKLRDAVDASTLDKMGDLVEQAKKVVPTHPHPLVPGTATAQLL
                     AGPWAATVDKVRDLLGGR"
     gene            9603..10040
                     /locus_tag="VLR27_00575"
     CDS             9603..10040
                     /locus_tag="VLR27_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01963.1"
                     /translation="MPELIQDHQWEPGARPRVLVEAVEWGTREHAAAALRDHGYDTVT
                     CPGPEGAGQRCRLAAGEGCSAAEGVDVVVHALRPWDPRNVEALRALRRRLPGLRVVVE
                     VAAPDVARQNLDLEGCVVLEAPASAAGLVAAVGRALGAQPEGG"
     gene            10123..10416
                     /locus_tag="VLR27_00580"
     CDS             10123..10416
                     /locus_tag="VLR27_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01964.1"
                     /translation="MNSTLTRRIAGAGFAAALLLGGAACGDDDDTTEEIVDTETETNT
                     EDVDVTETEGVTETDTETLELETETQTETVEETNTETDTEVVEEETETVTEED"
     gene            10524..11087
                     /locus_tag="VLR27_00585"
     CDS             10524..11087
                     /locus_tag="VLR27_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HSP01965.1"
                     /translation="MGSLLAAMGVVFLAELGDKTQLVALGFGARHRLGPVLAGVALAY
                     AATNLLSVVVGGLLGAALPTRALGLGGGVLFIGFALWSLRPEEDDDEEGVAAAAGHGR
                     VVLSVAAAMFVAELGDKTMLATATLAAQGNPVLVWIGATVGIIASGSVGVFVGRATGA
                     RLPERTIRIGSAALFAAFGIALIALNI"
     gene            11199..11648
                     /locus_tag="VLR27_00590"
     CDS             11199..11648
                     /locus_tag="VLR27_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSP01966.1"
                     /translation="MRAIEGIRRAGVSIGPDGTMRDAAELMERSGVGALAVLDGDHLV
                     GIVTDRDLVRRGLARRLEPEARVDSLMTSPAVTIEADADLRTAYAIFRENAVRRLAVT
                     ERGRFVGMLAIDDLLINLSADLADLVRPVTAETIYGHRDSPVPATTT"
     gene            11651..13375
                     /gene="acsA"
                     /locus_tag="VLR27_00595"
     CDS             11651..13375
                     /gene="acsA"
                     /locus_tag="VLR27_00595"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003313.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HSP01967.1"
                     /translation="MGTAGERSEVWRRARAALDGLPGDRGLNIAHEAVDRHLGTDREG
                     RCAIRWLGRHDEREDLTYADLAERTARFANVLADLGVGAGDAVFALSGRTPLLYVAAL
                     GTLKHRAVFSPLFAAFGPEPVRTRMQIGRGRVLVTTEAQYRRKVAALRTDLADLEHVL
                     LVDVDAASAPAGTHSLPALLDAASSTYEIGPTDPEDVALVHFTSGTTGTPKGAVHVHE
                     AVVAHHATGRLALDLRDGDVFWCTADPGWVTGTSYGIISPLTHGATLIVDEGEFDAER
                     WYGILGDEGVDVWYTAPTAVRMLMRAGAAAVPCGALARLRFVASVGEPLHAEAVAWGQ
                     EALGTPIHDGWWQTETGGIMVANQPGQEIRPGSMGRPLPGVGAAVLAVDEHGELRRDS
                     SGDPVRCGPDETGELALRTGWPSMFRGYLDQDERYRRCFADGWYRSGDLVREDADGYL
                     WFVGRGDDVIKSAGHLIGPFEVEKVLLEHPAVVEAGVIGVPDPVAGEVVQAFVSLAEG
                     HHPGDELRRELLGHARSRLGPAVAPRMIAFDQDLPKTRSGKIMRRLLRARALGLDEGD
                     TSTLEVGR"
     gene            13372..14358
                     /gene="pdhA"
                     /locus_tag="VLR27_00600"
     CDS             13372..14358
                     /gene="pdhA"
                     /locus_tag="VLR27_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HSP01968.1"
                     /translation="MTRLDPAHGRHLLEQMLRVRRMEERCAELYTQSKIRGFLHLYVG
                     EEAAATGVIEQLQPDDAVVATYREHGHALLKGTPMSAILAEMYGVAEGCSGGRGGSMH
                     LFDMDHRVYGGNAIVGGGLPVAVGLALADQLTGRQRVTACFFGEGAVAEGEFHESMNL
                     AALWDLPVLFCCENNLYAMGTALARSESETDMAAKAAAYEMPAWPVDAMDVVAVERAA
                     ARAVTAVRNGGGPHFLELRTYRFRAHSMFDPERYRDKDEVRTWRERDPIDLLVGRLGE
                     EGSLDEGELAGIEERVASEVEAAVAFAEAGTPEPVETLTRHVTSEPTGGALR"
     gene            14355..15335
                     /locus_tag="VLR27_00605"
     CDS             14355..15335
                     /locus_tag="VLR27_00605"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HSP01969.1"
                     /translation="MSTTTYREAMHDALAAALRDDDRVFLMGEDVGAYGGCFGVSLGL
                     FEEFGPERIRDTPLSEAAFVGAGIGAALGGMRPIVEIMTVNFSLLALDQIMNNAATLL
                     HMSGGQCNVPIVIRMTTGAGRQLAAQHSHSLEGWYAHIPGLRIVVPATVDDARWMLQT
                     ALDDPDPVLIFEHGALYGAKGELTDDLGPVDLDRAAVRRQGTDVSLITYGGTLPTVLA
                     AAEELAGRGIDAEVIDLRTLRPLDDDAILGSVARTHRAVVIDEGWRSGSISAEISARI
                     TESAFYDLDAPVGRVCSAEVPMPYARHLEIAALPSVDGVVQAVEELGLRG"
     gene            15328..16683
                     /locus_tag="VLR27_00610"
     CDS             15328..16683
                     /locus_tag="VLR27_00610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HSP01970.1"
                     /translation="MGEFCMPSLGADMDEGTLLEWLVAPGDRVHRGDIVAVVDTEKSD
                     IEVEVFEDGVVGELLVDVGATVPVGTPLARIEPASGRTGSAAPPPTPTPAPAPAEGPP
                     RGVATTARTAGRVLSPIVRHLAEERHLDLQHLVGTGSGGVITRHDVEAAVAPPPDPTP
                     TRAERSVVRPTPATGRTRSSPYARRLAAERGLALTAVPGSGPGGAVLAADVPDAAPVD
                     RDAGMRDAIARAMSRSKREIPHYYLQTTIDLGRALDWLRLRNEERPVVERVLPAVLLL
                     KATAVAAAEHPALNGSWEDGHRPAADVHLGVAIALRGGGLVAPGIDAADRMSLAELMA
                     ALRDLTQRARAGRIRQREWTGPTITVTNLGDLGVDAVWGVIRPPQLAMVGFGGVHDEV
                     LAVDGAPAVRPVVVASLAADHRTSDGIEGAAFLSRIAALLQEPDALDRTAPHPTGGVP
                     S"
     gene            16680..16934
                     /locus_tag="VLR27_00615"
     CDS             16680..16934
                     /locus_tag="VLR27_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HSP01971.1"
                     /translation="MTALTQADAHGLIAKILADIAPEVDLADARSDGQLQEELGLDSM
                     DFLNLMIGIHDETGLTVPERDYPELSTLDGAVRYLTERTA"
     gene            complement(16941..19772)
                     /locus_tag="VLR27_00620"
     CDS             complement(16941..19772)
                     /locus_tag="VLR27_00620"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HSP01972.1"
                     /translation="MTSSRDPWADADVDRFGIFVPAIMSAGLLFSIVTLLRDAVHGRR
                     HRHRDRRVEIAAAATAGHVTTGASATAALEQVLRPRSAYLLTALTCIGAAMYVVIGAV
                     GNYGRDNGYVSDIAWLLALSVITGFLLLWLGIGAGLLYARYPDTPAWATELLISTPLG
                     QAPDSDRAGSRRRFLLGWSATVVGTTFALITLSVGSSRGAFADADERLSDTVRAWEWV
                     DQLEGVHLLGRTEVAVAAALLIGVATLRCLPFAAAYAGAVVASLGATTLVREVIERDR
                     PPGTIMTGLEDSYPSGHMVQSVVLAGLVPLALLVLTRSALLARVAGVLLAVAIGLGAL
                     VRVQRGLHWPTDVLGGLALGVTIVLVVRWALARPALHARCRGCPWSPTPGPAHTFVHL
                     PDLAHGLLGLASRAVALVAIATFGILALTIGIPRNPRGESVAAPVEVWGTLGTLALLA
                     LALALSLRHPLLGAIGLAGGGLVLGALSSVAYHPLVSVAVAASFGIPAIGLWLTWQRG
                     RTRRAIVVVAVLCALSGTSVYATAATVHDHFYGPAHPVSDTVALPVDLVEWAWSGGVR
                     PDGFQVVARLDGDHDEAVLVVTPDAGGPVTRSEPVAVSAERVVRLEADGLAAGTDHRW
                     SVEVDGRVDGSRGSGRTRTAASGPTDVRIAVASCARTGSSGQVFDAIRAADPDLFVHM
                     GDFHYGDLATNDLDRYRALLERVVTAPSQAALYRAVPIAYVWDDHDYGPNDADSRSPS
                     RDAARTGYRELVPNVAAPTGPINQAFTLGRVRVVMTDTRSAKTPDSMLGDRQLRWLLD
                     ELRSSSRTHAVVLWVNSVPWIAAEEPGRDDWGGYPEEREEILAAIRDASIENLVMVSG
                     DAHMVALDDGTNSGGFPVLHAAALDRPGNVRGGPYSDGAFPGAGQYGLVEVDDDGGGH
                     VEVTLSGRTWEDELLVSRTFRFAVPRPAR"
     gene            complement(19769..22135)
                     /locus_tag="VLR27_00625"
     CDS             complement(19769..22135)
                     /locus_tag="VLR27_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HSP01973.1"
                     /translation="MSSRVVLLGDGGEHGADLGGKGASLARMVGWGHPVPRTAVVPIT
                     EYRRVASDPELGSYLDALATATEPPTAEEVDRRFLAVPLPEGLEDAIRSMAPALGPRI
                     AVRSSASSEDLRDQSFAGQYRSLLDVPTTGPDLLDAIRLVWASLWHPAPWAYRRAWGI
                     PHEDAAMAVVLMEMVPSERSGVLFTVDPGRPTDDLRVELVSGLGEALVSGERTPDVWH
                     VARSGSLPAGAPGYFAALRDGAMALEAAEGVPLDVEWAWDGGRLWLVQSRPITVDAAE
                     GDGFDTPVDDHELTSEGISEMLPGVLPPLRWGVASFLVEEAFRNVLSSLHALPDGVTS
                     PHAFVRRVRGRAVLDLDLLKAAAEQIPGASASDIEAQYFAEATTPPAVQPRASWMRLL
                     RRDLRAMATRRRSRFDGDVVIAVARAVDADPAPLARLDDRQLLSRRLRLLDLGVRAMT
                     AELGVAAAAAGAYAGVEQVLGRYLDAADAARHTQEVTLGATGVRTPAPTASRSVFAGP
                     TWSEAPQLLDRHGTRARPDAGSALADLEEILVADPRWRRTRVLSGQVVDVRLHMLRRT
                     IGDAIEDLGRREGVKAAVLALGGHVRLIHLEAGARLVDRGLLDAAEDVELLGEDELRR
                     ALLDGAAVPRGMLGRRRRHLARWREEDELPSRFTGMPPVTVSEPPTGDCLTGIAASPG
                     RFTGRAVVVRDPTDAPPPPGSVIVARATDAAWSPLFIDAGAVVVERGGPLSHAAIVAR
                     ELGLPAVLDVHGATRHLDGRTVTVDGSAGIVAVHEDERTPPPTEVAVG"
     gene            complement(22132..22437)
                     /locus_tag="VLR27_00630"
     CDS             complement(22132..22437)
                     /locus_tag="VLR27_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01974.1"
                     /translation="MPGSSPRALLVLVLATVVSFVGLVDATVSRTWDFAVVFALAVGL
                     QLLLVVRTQTGRAGLTVRRDLAAWASEQAVATGETADEVVDRCVAAVRADVVDRPGP"
     gene            22582..23478
                     /locus_tag="VLR27_00635"
     CDS             22582..23478
                     /locus_tag="VLR27_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01975.1"
                     /translation="MDAGPDRETVLPSTVRLPDGRAVVVRNAGPADVPGLMALFERLG
                     PDDRYRRFFSGFRPDDDFVRALVMRPPEEGRLLVAEVTGPDGTDIVAEAEFARLSDGD
                     GELALTVDRGWRGWLGPFLLDALLQVAAADDIPDLRAEVLAQNRPMLALLRARGCAMV
                     ESGDPCVTEVVVGTHGAAPSWAPGSAHPRVLLEAAGGRWQLTTKLGEAGVPVLRCPGP
                     GVRPANVPCPLLQGERCPLADGADVIVHALDPDDPRSVTVLDAHRRAGGRVVEVGPPG
                     TDDVDAIVAEVVRAPTRAPDGG"
     gene            23480..24904
                     /locus_tag="VLR27_00640"
     CDS             23480..24904
                     /locus_tag="VLR27_00640"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HSP01976.1"
                     /translation="MLFPTIRFALFFMVTWGVLLGVRDRPIARRGVLLGASWVFYAAW
                     DWRWLALLWAMIGVNHLAVRGVAWAPTAAGRRAALALGVGADLALLGTFKYYDFFAES
                     MVGTLGLLGVQASAPLLQVALPLGISFLTFQAISYLVEVHRGVTAPAPLLDEAVWLSF
                     FATVVSGPITRASELIPQLDRVPRAVDPGASFALVIRGLFKKVVLASYLATAVVDEAF
                     ATPAAFSATEVLFAVYAYGALIYVDFSGYTDIARGVAGLLGFHLPENFDAPYRAASVQ
                     EFWTRWHMTLTRWLRDFLFTPLGLRYGRRRVIGPLLPLLIMLAAGLWHGAAWTFVVFG
                     GIHGLALVAERADRVRRRRLGLGRAPDTPGRRLVRHVVTLQVVSLAWLFFRAESMGAA
                     WAMLHRIATGPLATGQVTLLAVATVVGIYGVQLVRAPAVAERAQAWATHAGPVTQAVG
                     LAGALTVVDVLGPAGVAPFIYFGF"
     gene            24904..25962
                     /locus_tag="VLR27_00645"
     CDS             24904..25962
                     /locus_tag="VLR27_00645"
                     /inference="COORDINATES: protein motif:HMM:NF016218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HSP01977.1"
                     /translation="MALTTPATATFPPDELPAGPSPRSGRVWRPFAVAAVALVLAALL
                     NADELRQTAERQPVGWEREALVDLAAIPKEVADATRLDRPRAWAESLLGTQEPAAIAV
                     GPPEPPPARPPDTQVPVTDPAGSSTSTSTTTTVPERRTPTAGAPLQVAVIGDSMMQVL
                     GQSIVNGAAGDDRLETSLEYRVSTGLTRPDYFDWPGRITELLAAGPLDAVVVMYGAND
                     AQGMVLPTGSEPFGTPVWLDEYRRRVGWVMDALVAGGAETYWVGQPVMRSDSFSARMA
                     SLNEVYASEAALRDRVTYVDAWAVLSVDGAYTSHIDDGGGPTSVRQSDGIHLSRAGGD
                     RLAAVVLDQIRSDWRLDP"
     gene            25959..26690
                     /locus_tag="VLR27_00650"
     CDS             25959..26690
                     /locus_tag="VLR27_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01978.1"
                     /translation="MRPLRTIGVVVTCLALVAGCGGSSTQTATVRSTTTATTSTSSTT
                     VAPSTTAQPVPSTTTSAPTTVAPSTTAPTTTPPTTARPPSPTGRWVFVLGDSALLGAR
                     DSVPAALPGWQVVFDAVGSRRLPQAIEVLRARRSEIGSVAVIQQGNNYLESEGSFGAQ
                     IDEAMRVLDGVDRVVWLTVAEKWPSRVEINQHIRAAAGRWPQIVVAEWAPLIAAHPEY
                     TEDMLHLSAAGRVAIAELIAGAVTA"
     gene            complement(26712..26894)
                     /locus_tag="VLR27_00655"
     CDS             complement(26712..26894)
                     /locus_tag="VLR27_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01979.1"
                     /translation="MSETVLFVVGAVVFAITVYGAVMAGGLALTKSSIIENEQYRRRV
                     PDDQVAAGHLPTDSKF"
     gene            complement(26891..>27097)
                     /locus_tag="VLR27_00660"
     CDS             complement(26891..>27097)
                     /locus_tag="VLR27_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01980.1"
                     /translation="PRHLVVRLPFLVAGFASFVFSVYLYFVADETTAGIFVGLWVPSI
                     HSLGTLLLTPVHADARDLADAVRS"
BASE COUNT         3714 a         9929 c         9858 g         3596 t
ORIGIN      
        1 tggcagcgct tcgccaacct gcgcaccctc tacggctgga tgtgggggca cccgggcaag
       61 cagctcctgt tcatgggctg cgagttcgcc caggagcggg agtggtccga gtcgcgcagc
      121 ctcgactggc acctgctcga cgacccccgc cacgccggcg tgcagcagct catcagtgcc
      181 ctgaaccagg tccacgctga gcacccggcc atgtggcgtc gggacaccga gccggccggc
      241 ttcgactggc tcgacgcgtc ggacgccggc cagtccgtgc tcgccttcca gcgcaacggg
      301 cggcccgacg aggacgatcc cgtcgtctgc gtggccaact tcacgccggt gccccgccac
      361 ggctaccgca tcggcctgcc ccgcgccggc gcctggcgcg aggtgctcaa caccgacgac
      421 gagcgcttcg gcggggccgg catcgtcaac agcgcgatcg agagcgagga cgtcccctgg
      481 cagggccggg agcagtccgc ggtggtcacc ctgccgcccc tcggcgtcgc ctggttcgcg
      541 cccgaaagcc cttgacgcgc ccggatccga ggcgcaacat cgtcatacag agcgaacgtc
      601 gaagggacga ttgcccggag gattgccagc ggttccgccc gcccaagaca gcgctgcggc
      661 gctgtggcca cggagcaccg gccgtgcagc caccgagcat cggtcccagg acacgagccc
      721 ccgacggggg ctcgtgtcgc gtccggtcct ccgaagtccg gaccaggccg ctcgccggta
      781 ccgtcgtggc ccgtgacgac cgaggtccag acctgctacc gacacagcga ccggcgcgcc
      841 ggcgtgcgct gccagcgctg cgagcggccg atctgcccgt cgtgcatgaa ccaggcctcg
      901 gtcggcttcc actgcccgga gtgcgtcaag ggcgccggcc agaaggtctt cacggcgcgc
      961 acgatgccgt cgtcggccaa cccgatcgtc accacgacgc tcgtggccat gaacgtcgcc
     1021 atctacgtgg cgtccagcct cggcgccggg ctcagcgagc cctccttcca ggtccagctc
     1081 gactacgccc tcatcggccg gggcctgtcg tccgagggcc tcctcggcgt cgccgagggg
     1141 gagtggtacc gactgatcag cggcggcttc ctgcacggca gcctcgtgca cctcggcttc
     1201 aacatgttcg tgctgtgggt cctcggcaag cagctcgagc cggcgctcgg tcgggtcggc
     1261 ttcgccgccg tgtacttcgc gtcgatgctc gccgggtcct tcggcgtgat gctgctcgac
     1321 cccggggccc tcaccgtcgg tgcctccggt gcggtgttcg gcctgttcgg ctacgccgtg
     1381 gtcgcccagt tcgtgcgggg catcaacccg ctccagaccg gcctcggtgc ggtcatcctg
     1441 ctcaacctcg cgttcacgtt cctcgtgccc ggcatcagca tcggtggcca cgtcggcggc
     1501 ctcgtcggcg gcctcgtcgc cggcgccctg cacgacctcg tgcgtcccaa gctcggcgcg
     1561 ccgccccagg tcggcgtcgc cctcgtcacc gccttcggcg tggcgtgcgc cgtgggatcc
     1621 gtcctcgtcc tctagccgcg ctcgccgacc agcggctgga gcaggcggcg gaggccgagc
     1681 tcgtagagct cctcggctcg gtcgggggcg gtgctcatcc cggtgatctc cgtgagcacg
     1741 tagccgtgga ccgtcgcgtt cacgtggtac gcccagtcga aggcgtcgcc ctcgctcagg
     1801 tcgggacgga tcgcgtgcac gtggtggacc aggcggagga acgactcgta gccccgctcg
     1861 agggcggcgt cgcccggcac gacgtcgggc acggcgcggc cgaacatgac caggaactgg
     1921 gtcgggttct cgagggccca gcgccggtac gcccgaccgg aggcgccgac gtcgccagag
     1981 gcgtacgccg gggccagggc ggcgtcgaag gactcgaagc cgtcgatgaa gatcgcatcc
     2041 accaggccgg ccttgccgcc gaaccaggtg tagacgccgg tggtcgagca gccggcctcc
     2101 tcggcgatgc gccgcaccgt gaaggcggct tcgccgtcgc gggccaggac ggcgagggcg
     2161 gcggacagga tcgcgctgcg ctggtcgggg atcggggttg acacggcgtc ggccacgtaa
     2221 caatgttacg cagataacaa agttacgcga cgaaccccac gggagatccc atgaccgcca
     2281 ccgagcagcc caccgaccag tcgggctacg gcaaccggta cctgaccggg ccgtacgcgc
     2341 cggtcaccga ggaggtcacc gccttcgacc tgccgatcga gggcgagctg cccgaggagc
     2401 tggccgggcg ctacctgcgc aacggcccga accccatcga cgacgtggac ccggcgacgc
     2461 accactggtt cctcggcgac gggatggtcc acggcgtgcg cctgctcgac gggcgggccg
     2521 agtggtaccg caaccgctac gtgggctccc agcacgtcgg tgaggtgcgc ggcacgggcg
     2581 acgtccccgg tccgaactgg aacgacagcc cgggcgggcc caacaccaac gtcggcggct
     2641 tcgcgggcac cacgtgggcc atggtcgagg ccggtggcac cccggtcgag ctcacctacg
     2701 agctcgagac cgtgggccgc aacgacttct ccggcaccct gcccggcgcc ttcaccgcac
     2761 accccaagct cgacccggac accggcgaga tgcacgccat ggtctacgca cccgccaact
     2821 ggatggacca catccagtac gtcgtcgtgg cccccgatgg ccgggtccgc aaggcgctcg
     2881 acatccccct cggcatgacg atgctgcacg acatgtcgct cacccagcgc tacgcggtga
     2941 tctacgacca gccctgcacc gtcgacatcg agctggccat ggccggcgac tccttcccga
     3001 tgcggtggaa ccccgactac ggcaaccgcc tcgggctgct gccccgggag ggcgcagccg
     3061 aggacatcat ctggatcgac gtgcccatcg gctacgtgtt ccacccgatg aacgcgttcg
     3121 acgacgcgga cggcaaggtg gtcatcgacc tgtgccgcta cgacaagatg ttcgacgtcg
     3181 acgtgctggg tccgttcggc gacggcgggc acgccaccct ggagcgctgg gagctggacc
     3241 cggtggcccg cacggtttcg accacggtcg tcgacgagcg ggcgaacgag ttcccccgtc
     3301 accggggatc gctcacggcc aagccctacc ggtacggcta ctgcgcctcg ccgtcgctcg
     3361 acgacggcgc cggctggccc accctcaagc acgacctgca gaccggccag cggtgggagt
     3421 tcgaccacgg cgcgggccga gccgcggggg agccggtgtt cgtcgcccgc cagggcggca
     3481 ccgacgagga cgacggctgg ctgctcacgt tcgtccacga cctcggcagc ggcgacacgg
     3541 acttcgtggt gatggatgcc gctgacctgg accgtcgcga ctacgtggcc cgcgtccgac
     3601 tgccccagcg ggtgccgctc gggttccacg gcaactgggt gagcgaccgg gcggtgccgc
     3661 ccccgatgtg acgctcgtct ccccggtggg ccaggatcgg aacgtgaccg atcactaccg
     3721 cgaagcatgg gacgagctca cggcaccagg gggctcattc gagatcgaga cgatcgacgt
     3781 ccggggcgtg ccgatccagg tcttcaagag cgcgccgccg aacatgcggg tgctctggga
     3841 gctctccgcc ggccacggcg acaaggacta cgtcgtctac gaggacgagc gctacaccta
     3901 cgccgaggtg cacgcccagg tccgcgccct ggcccacctg ctgcgcgaga cccacggggt
     3961 ccgggagggc gaccgggtcg ccatcgccat gcggaactac cccgagtggg tcgtgtcgta
     4021 ctgggccacc ctcagcctcg gcgccgccgc cgtggggatg aacgcgtggt ggaccggtcc
     4081 ggagatggag tacggcctca ccgactcccg tcccaaggtc ctgatcgccg acgacgagcg
     4141 cctcgagcgg gtcctgcccc acctcgacgc tgtgcgggcc gagcagccca tgcacgtcat
     4201 caccgtgcgc tccgaccgcg agctgcccga cgacgcggcc cgctggaccg acgtggtgcg
     4261 ggccgaggac gcccccgacg ccctccccga ggccaccatc gaccccgacg acgacgcctg
     4321 catcttctac acgtcgggca ccaccgggtt cccgaagggt gcccagctca cgcaccgcgg
     4381 ctgcgtgcac aacatcctcc acatcgtctt catgaccacg gccgccggca tggcggccca
     4441 gaaggcgggc accgcaccgg cccccgaccc ggatgccgcg ccgtcgcagg cggtgttcat
     4501 ggccccgacg ccgctgttcc acgtcaccgc caacaactgc ctgctgcacc cgtgcacgct
     4561 ggccggtggg cgcatcgtgt tcacctacaa gtgggatccg ggccgagcgc tcgagctgat
     4621 cgagcgggag cgcgtcacca acttctccgg cgtgcccacc atgagccgcg agctgctcgc
     4681 ccacccggac tgggagaagc gggacacgtc cagcgtgcag ggcatgggtg gcggcggcgc
     4741 cgcgctgcag cccgacctgg tcgacaagat cgacaagtcg ctgaagtccg gtgcgccctc
     4801 caccggctac ggcctcaccg agacctgcgg catcgtcacc gccaactcgg ccggcaacta
     4861 cgtggccaag ccggcgtcct gcggtccggt ggtgcccacc ctcgaggcca agctcgtcga
     4921 cgaggccggc aacgacctgc cgcccggccc ggacaccgtc ggccagctct gcgtgcgggg
     4981 ctcggtggtg atcaagggct acctgaaccg ccccgaggcc acggccgagg cgatccagga
     5041 cgggtggttc aacaccggtg acatcgcccg catcgacgag gacggcttcg tgttcatcgt
     5101 cgaccgggcc aaggacatgg tcatccgggg cggcgagaac atctactgct ccgaggtcga
     5161 gtcagcgatc tacgagcacg acgacgtcgc cgaggtcgcg gtgttcggcg tgccggacga
     5221 gcgcctcggc gaggaggtcg gcgccgccat cgtgctgcgc aacggtgcct cgctcgacga
     5281 ggagggcctg cggtcgttcc tgagcgagcg ggtcgccaag ttcaagatcc ccgcccacgt
     5341 gtggttccgc accgagccgc tgccgcgcaa cgccaacggc aagttcgtca agcgcgacct
     5401 gcgcgacgag tacgtcggcg cctaggccgc cgaggcagct tcgagcacgg cggcggtggc
     5461 gacgaggacc tcctcctcgc cccgccgccc gatcagctgc acggccgtgg gcaggtgccc
     5521 cggtcgggcg accggcacga cgatcgccgg gttgcccgcc aggttcacgg ccacgttcgg
     5581 ggcccgggtg tcgagccgct ggggctcggt gagcagcggg ggctcgtcca tgatgccggc
     5641 ggtggcgatg accggggtcc gcgccagcgc ggcgtccagc tcggcgcgcc acggctcggc
     5701 atgggcccga gcggcgtcga gcgcgccctc ggtgatcccg gcccccttcg cgagccggtc
     5761 ccgggtctcg tcaccgagct cgtcggcgtg gtcgcgcacg agcgaggcgt gcacgcgggc
     5821 ggcctccccg aacaggatcg ccgtgccggc gtccgcggcc tcctcccagc cggggagcgc
     5881 ggtgggcgcg atgtggagcc cgctcgcgtc gagcagccgg tcgatggcgg cttcgatctc
     5941 ggggtgggcg ccggggaggc ggaaccggcc gagcgacggc gcagcggtcc cgggcgcgaa
     6001 gcccgcttcc agcagcgcca tgccggcgac gacgccggcg acggtggtgg ccatgggccc
     6061 gaccgtgtcg agcgacgggg ccaggggtcg cacgccggcg gtggggatgc gaccgaaggt
     6121 cgtcttgagc ccgaccacgc cgcagcaggt cgacgggatg cgaacggagc ccgcggtgtc
     6181 ggtgccgatg gcgatgtcgg cctcgccggt ggccaccgcc accgccgagc cggacgacga
     6241 gccgccgggg atgcgggtcg ggtcgagcgg gttctgcggc gtcccggtgt aggggttgat
     6301 cccgctcccc ccgaaggcca gctcgtgcag gttgaccttg ccgacgatgc ggccgccggc
     6361 ggcccggatg ctggtgatgc acgcggcgtc agccgtggcc ggcctcgccg cctcggcgac
     6421 cgggcgtgac gcgcaggtgg tgggggtgcc ggcgatgtcg atgagatcct tcaccgcgac
     6481 cgtgggcccg cccccttcgt ccgggtcgag ccggagcagg aacgtggtca cgccgttcga
     6541 ccttcgacga tggccttgag ccggcgcagg ttccccttcc agatcacgcg catgatccgg
     6601 ccgccgacga tgccaccgat cggaccaccc atccaccagg ggaagacgag gcgctcggac
     6661 cactcgaacc gcgtcgccga ccgtcgacgg aggcgccgtc ccgccggtgc cagggtgaac
     6721 gccccctcgc cggtgaccag accgacgtgg cggaccccca tcgccgtgcc cggctcccag
     6781 cgggtgatct ccatcaggtc ggtgagccgg agcgggccga ccttggtgtc gcactcgaac
     6841 gtggtgccca ccccgctggt gtggtcggag gtgaaccgga tggcctcggc gtcggccatc
     6901 cactcgacgt gggagccgac gtcctcgacc gccgcccaca ccgccgccgg gctcgcgtcg
     6961 atggtggtgg agacggtgat cctggccatg gccgaacggt aggcacgccc gggtccgcgt
     7021 tcctgtcacc ccccgtgcgg atactggacc catggccgtc ctcctcgctc tcctcgccgc
     7081 cgtcgtggcc gccgggctgg ccgccctcgt ggtcgcccgg ctcaccacca cccagctgtc
     7141 ggcccgggcg gtggaggagc gggaggcagc ggtggctgcc gccgtcgacg ccgtcgtggc
     7201 ccaggccggg cacgccttcg tggccgagcg cgagcacacg ctgcgcgccg cggtggagag
     7261 cgccacgtcg gtggccacct ccaagctgca ggactccatg accgccggca gccgggagct
     7321 cgacctgcgc tcccgctcgt tcgagcagca ggtcacccac atcgccgatg cgctcggcac
     7381 gctcgggggg ctggtccagt cgctccagaa ggagcgggcc gagcagaccg ggtcgctcgt
     7441 ggcccagctc gagcacgtca cccgcaccca gcagtccctg gcccagcaca ccgaccagct
     7501 ccgccaggcc ctggccagcc ccaagcagcg gggccagtgg ggcgagcgca tggccgagga
     7561 cgtgctgcgc gccgccggct tccgcgaggg cgtcagctac cgccgccaga ccgcgatcga
     7621 gggcggcacc atccccgact tccggttcct gctgccccgc gacctcgagc tgcgcatgga
     7681 cgtgaagttc ccggtcgaca actacctgcg cgtgctcgag tgcgactccg acgccgaccg
     7741 cgcccggcac accaaggcct tcgtcgccga cgtgcgcacc cacgtgcgcg gtctggcggg
     7801 gcggggctac gtggaccacc gcgacacggt cgattgcctg ctgctgttca tccccaacga
     7861 ggccgtctac gcgttcctgc acgagtccga ccccgacatc gtcgacgtcg ccctcggcca
     7921 ccaggtcgtc ctgtgctcgc cgagcacgct gttcgccgtg ctcgccgtgg tccggcgctc
     7981 ggtcgagcag ttccagctcg agcgccgcag cgacgacatc ctccgctgcc tcggcggcct
     8041 gaccggggag tgggacaagc tggtcggtgc catcgagaag gtcgggcgcc agctcgacct
     8101 cacccacaag agcttccacg atgagctcaa cggcacccgc cggcgcgtgt tccagcggtc
     8161 actcgacgag atcgagtcgc tccagtcggc gaccgagcgc ccggtgctca gcgcgggcga
     8221 ggccctggcc gcgcagaagg cggccggcga cgtccgggcg ctgcccgtcg acgacagcga
     8281 ccgtggtgtg gccgaggccg gctgacgccg tggtggcgtg ccgccaccgc tgcggccgga
     8341 tgttctagaa cgagggggcg cccgggaacc gacccggcgt acccaccgtc gagaccggga
     8401 gaagaccagc gaatgtccga ctacgagatc ccgaagcctg agcccaagga gatcgactcc
     8461 gtcaagtggc tcgagtggaa ggacgtgatg gagcgagcca ccctcaaggg ccatccgtac
     8521 aaggacgagc gctgcgacaa ctgcttgtac tacctggatc cggacgagga gatctcgtac
     8581 tgctggcacc agaccctgcg catcctcgtg ggtggcgact ggtggtgcca gtggtgggag
     8641 ccgatcgagg aggagtgatc atggccggcg acgagctgcc cgacgacgtc cccgaggccg
     8701 acgcgctgga acagcgccga gcggtcgacg accagcccga tgccgacgct gacgccgtgc
     8761 tcgacgacga cagcttcgag gtgcccgagg ccgacgccct cgaccaggcc cgggccgtcc
     8821 ccaccgacga cgaacccgac ccggagggct gagcccaccc gcctgcgccc cggacgcgat
     8881 cgcgtccggg gcgtgtcgcg tcagcgtccg ccgagcaggt cccgcacctt gtcgaccgtg
     8941 gccgcccatg ggccggcgag gagctgcgcg gtggccgtgc cggggacgag cgggtgcggg
     9001 tgcgtgggca ccaccttctt ggcctgctcg accagatccc ccatcttgtc gagggtcgag
     9061 gcatccacgg cgtcgcggag cttggggagg acgtcggact cctcctcctc gacgtggtgc
     9121 ctgatcgtct cgatcagcgc ttccatggcc tcaccgtgct ccgcggagcc ggcgtcgagc
     9181 ttctcgaggt cggccaggag acgcttggcc tgctggtgct cgtcgatggc ctcgtcggcc
     9241 atggcgttcc cgccgtcgac cttggcccgc agcagcgggt agacgaactg ctcctctacg
     9301 gcggcgtgga cggagaggtc gtggacgatc tgcttggtca gctcgtcgat cgattcgggc
     9361 ttgtccgaca ggttgacgcg gcggaacagc tcgtcaacct cttggtggtc cttctccagc
     9421 agcgtgatga cgttcattcg gggtccttcc ggggatgggt ggtgtgcgct gctctccgtt
     9481 cccggcctgg cccgggccca tgtcggaggg gacgttggtc cctggtgcgc cctggctggg
     9541 tggtcgacca tcgcggaggg accgggaacc gacccggcac gtggagaagg ggagcgcgcg
     9601 cgatgcccga gctcatccag gatcaccagt gggaacccgg cgcccgaccc cgggtcctgg
     9661 tggaggccgt cgagtggggc actcgcgagc acgccgcagc ggcactgcgc gaccacgggt
     9721 acgacacggt cacctgcccc ggcccggagg gcgccggcca gcggtgccgg ctggcggcgg
     9781 gggaggggtg ctcggccgcg gaaggggtcg acgtggtggt ccacgcgctt cgcccgtggg
     9841 acccgcggaa cgtggaggcc ctccgcgcgc tgcgacgtcg cctcccgggt ctccgcgtcg
     9901 tcgtcgaggt cgccgcgccc gatgtcgcgc ggcagaacct ggatctcgag gggtgcgtgg
     9961 tcctggaggc gccggcgagc gccgcggggc tggtcgcggc ggtcggtcgg gccctcgggg
    10021 cgcagcccga gggtggctga gcacgcgcgg accgggttct atcgatctcc tcctcgggta
    10081 gatggcgaac tccgcgccat cccccctacg gaggtatgac agatgaattc gaccctgacc
    10141 cgccgcatcg ccggtgccgg cttcgccgcc gcactcctgc tcggaggcgc cgcctgcggc
    10201 gacgacgacg acacgacgga agagatcgtc gacaccgaga ccgagaccaa caccgaggat
    10261 gtcgacgtca ccgagaccga gggcgtcacc gagaccgaca cggagaccct cgagctcgag
    10321 accgagaccc agaccgagac cgtcgaggag accaacaccg agacggacac cgaggtcgtg
    10381 gaagaggaga ccgagacggt caccgaagag gactgacccg cctcggccgg tcccgccggc
    10441 tgaagcgatc gcacaccggg gcgaagggcg cagcgcacgc tgcgcccttc gtcgcgtccg
    10501 ggcccgcctc gtaggttcgc agcgtgggat cgctgctggc tgccatgggc gtcgtcttcc
    10561 tggccgagct gggtgacaag acccagctgg tcgcgctcgg gttcggtgcc cggcaccggc
    10621 tcggtccggt gctcgccggc gtggccctgg cctacgccgc cacgaacctg ctctcggtcg
    10681 tggtgggcgg gctgctgggg gccgccctgc ccacccgggc cctcggcctg ggcggcggtg
    10741 tcctgttcat cggcttcgcg ctctggtcgc tgcgacccga ggaggacgac gacgaggagg
    10801 gcgtggccgc cgctgccggc cacggccgag tcgtgctgtc ggtcgccgct gccatgttcg
    10861 tcgccgagct cggcgacaag acgatgctgg ccaccgcgac cctggccgcc cagggcaacc
    10921 ccgtgctcgt gtggatcggg gccaccgtcg gcatcatcgc ctccggttcg gtcggggtgt
    10981 tcgtcggtcg cgccaccggc gcccgcctgc ccgagcgcac catccgcatc ggctcggctg
    11041 cgctcttcgc ggccttcggg atcgccctca tcgccctgaa catctgaacc gggcaccagg
    11101 gcaccagcgc ccctgggggg catgaccttc tgccctgtcc ccggccccgg gacaggagtc
    11161 tgatcggggg agagatcgtc acccgaggag gaggcaccat gagggccatc gaagggatcc
    11221 gacgtgcggg cgtgtccatc ggcccggacg ggaccatgcg cgacgccgcg gagctcatgg
    11281 agcgatccgg cgtcggggca ctggccgtcc tcgacgggga tcacctcgtc gggatcgtca
    11341 cggaccggga cctggtccgc cggggtctgg cccgtcggct ggagccggag gcgcgggtcg
    11401 actcgctgat gaccagcccg gccgtcacga tcgaagccga cgccgatctg cgcaccgcgt
    11461 acgccatctt ccgggagaac gccgtccgcc gcctcgccgt gaccgagcgc gggcgcttcg
    11521 tcggcatgct ggcgatcgac gacctgctca tcaacctgtc ggccgatctg gccgatctcg
    11581 tccgcccggt cactgccgag accatctacg gccaccggga cagcccggtg ccggcgacca
    11641 ccacctgatc gtggggacgg ccggggagcg gtcggaggtc tggcggcgcg cccgtgcggc
    11701 gctcgacggg ctccccggcg accgcgggct gaacatcgcc cacgaggcgg tggatcgcca
    11761 cctgggcacc gaccgggagg gtcggtgcgc catccgctgg ctcggacgcc acgacgagcg
    11821 cgaggacctg acctacgccg acctcgccga gcggaccgcc cggttcgcca acgtcctggc
    11881 cgacctcggc gtgggcgccg gcgatgccgt gttcgccctg agcggccgca caccgctgct
    11941 gtacgtggcg gccctgggca cgctgaagca ccgcgccgtg ttctccccgc tgttcgcggc
    12001 cttcggtccc gagccggtgc gcacccgcat gcagatcgga cgcggacggg tgctcgtgac
    12061 gaccgaagcc cagtaccggc ggaaggtcgc ggccctccga acggatctgg cggatctcga
    12121 gcacgtgctg ctcgtggacg tggacgccgc ctccgctccc gcgggcacgc actcgctgcc
    12181 ggccctgctg gatgcggcgt cgtccaccta cgagatcggc ccgaccgacc cggaggacgt
    12241 ggcgctggtg cacttcacca gcgggaccac cgggacgccc aagggagccg tgcacgtgca
    12301 cgaggcggtc gtcgcccacc acgccaccgg ccggctcgcc ctcgacctgc gcgacggcga
    12361 cgtcttctgg tgcaccgccg accccggctg ggtcaccggg acctcctacg gcatcatctc
    12421 gccgctcacc cacggcgcga cgctgatcgt ggacgagggc gagttcgacg ccgagcgctg
    12481 gtacggcatc ctcggggacg agggcgtgga cgtctggtac acggcgccca ccgcggtgcg
    12541 gatgctcatg cgggccggcg ccgccgccgt cccctgcggc gcgctggcac gcctgcgctt
    12601 cgtcgccagc gtcggcgagc cactgcacgc cgaggcggtg gcgtggggcc aggaggccct
    12661 gggcaccccg atccacgacg gctggtggca gaccgagacc ggcgggatca tggtcgcgaa
    12721 ccagccgggt caggagatcc gtcccggctc catgggacgt ccgctgccgg gggtgggggc
    12781 ggcggtcctc gccgtcgacg agcacggcga gctgcgtcgg gacagctcgg gcgacccggt
    12841 gcgctgcggt ccggacgaga ccggcgagct cgcgctccgg acggggtggc catcgatgtt
    12901 ccgtggctac ctggaccagg acgagcgcta ccgccgctgc ttcgccgacg gctggtaccg
    12961 atccggtgac ctcgtgcgcg aggacgccga tggctacctg tggttcgtcg ggcggggtga
    13021 cgacgtcatc aagtccgccg gccacctcat cggccccttc gaggtcgaga aggtgctgct
    13081 cgagcacccg gccgtggtcg aggccggcgt catcggcgtc cccgacccgg tcgccggcga
    13141 ggtggtgcag gccttcgtca gcctcgccga gggccaccac cccggcgacg agctgcgccg
    13201 cgagctgctc ggccacgcca ggagccgcct cggccctgcg gtggcgccac ggatgatcgc
    13261 cttcgaccag gacctgccca agacgcgcag cggcaagatc atgcggcgtc tgctgagggc
    13321 ccgggcgctg ggtctcgacg agggcgacac gtcgaccctg gaggtgggga gatgacccgg
    13381 ctcgaccccg cgcacgggcg gcacctgctc gagcagatgc tgcgggtgcg gcgcatggag
    13441 gagcgctgcg ccgagctgta cacgcagtcc aagatccgcg gcttcctgca cctctacgtg
    13501 ggggaggagg cggcggccac cggcgtcatc gagcagctgc agccggacga cgccgtggtc
    13561 gccacgtacc gcgagcacgg ccacgccctg ctgaagggga cgccgatgtc cgccatcctc
    13621 gccgagatgt acggcgtggc ggaggggtgc agcggcggcc ggggcggttc catgcacctg
    13681 ttcgacatgg accaccgcgt gtacgggggc aacgcgatcg tgggaggcgg cctgccggtc
    13741 gcggtcggcc tcgccctggc cgaccagctc accggtcggc agcgcgtgac ggcctgcttc
    13801 ttcggcgagg gcgccgtcgc cgaaggcgag ttccacgagt ccatgaacct ggcggcgctg
    13861 tgggacctgc cggtgctgtt ctgctgcgag aacaacctgt acgccatggg gacggcgctg
    13921 gcgcgcagtg agtccgagac ggacatggct gcgaaggccg cggcctacga gatgccggcg
    13981 tggccggtcg acgccatgga tgtcgtcgca gtcgagcggg cggcggcgcg ggccgtcacc
    14041 gccgtgcgca acggcggcgg gccgcacttc ctcgagctgc gcacctaccg cttccgggcg
    14101 cactcgatgt tcgaccccga gcggtaccgg gacaaggacg aggtccgcac ctggcgcgag
    14161 cgcgatccca tcgacctgct ggtcgggcgg ctcggcgagg agggatcgct cgacgagggc
    14221 gagctcgccg ggatcgagga gcgcgtcgcc tccgaggtgg aggcggcggt cgccttcgcg
    14281 gaggcgggca caccggaacc ggtggagacc ctcacccgcc acgtgaccag cgagccgacg
    14341 ggaggtgcgc tccgatgagc accacgacgt accgggaagc gatgcacgac gccctggccg
    14401 cggccctgcg ggacgacgac cgggtcttcc tgatgggtga ggacgtcggc gcgtacggcg
    14461 ggtgcttcgg ggtcagcctc ggcctcttcg aggagttcgg tcccgagcgc atccgggaca
    14521 cgccgctgtc ggaggcggcc ttcgtcggcg ccgggatcgg cgccgcgctg ggcgggatgc
    14581 ggccgatcgt cgagatcatg accgtgaact tcagcctgct cgccctcgac cagatcatga
    14641 acaacgcggc cacgctgctg cacatgagcg gcggccagtg caacgtgccg atcgtgatcc
    14701 gcatgacgac cggcgccggc cgccagctcg cggcccagca ctcccacagc ctcgagggct
    14761 ggtacgccca catccccggt ctccgcatcg tcgtgccggc gacggtggac gacgcccgct
    14821 ggatgctgca gaccgcgctc gacgaccccg accccgtgct gatcttcgag cacggcgccc
    14881 tctacggggc gaagggtgag ctcaccgacg acctgggtcc cgtcgacctc gaccgcgccg
    14941 ccgtccgccg ccaggggacc gacgtgagcc tgatcaccta cgggggcacg ctccccacgg
    15001 tgctcgccgc cgccgaggag ctcgccgggc ggggcatcga cgcggaggtc atcgacctgc
    15061 ggaccctgcg acccctcgac gacgacgcga tcctcggctc ggtggcccgc acccaccggg
    15121 cggtcgtgat cgacgagggc tggcgatcgg ggagcatctc ggccgagatc agcgcccgca
    15181 tcaccgagag cgccttctac gacctggacg caccggtcgg ccgggtctgc tcggccgagg
    15241 tgccgatgcc ctacgcccgc cacctcgaga tcgccgccct tccgagcgtc gacggggtcg
    15301 tgcaggccgt ggaggagctc ggtctccgtg ggtgagttct gcatgccctc cctcggcgcc
    15361 gacatggacg agggcacgct gctcgagtgg ctcgtcgcgc ccggcgaccg ggtgcaccgc
    15421 ggcgacatcg tcgccgtggt cgacaccgag aagtccgaca tcgaggtgga ggtcttcgaa
    15481 gacggcgtgg tcggtgagct cctcgtcgac gtgggcgcga ccgttccggt gggcacgccg
    15541 ctggcgcgca tcgaaccggc gtccggccgc accggatcgg ccgctccgcc ccccaccccg
    15601 accccggcgc cggcgccggc cgaggggcca ccacgagggg tggcgaccac cgcccggacc
    15661 gccggccggg tgctgtcccc gatcgtgcgg cacctggccg aggagcggca cctggacctc
    15721 cagcacctgg tcgggaccgg gtccggcggg gtgatcaccc ggcacgacgt cgaggcggcc
    15781 gtcgcgccac caccggaccc gaccccgacc cgagcggagc ggtcggtggt gcggccgacg
    15841 cccgcgaccg gtcgcacccg gtcgtccccg tacgcccgcc ggctcgccgc cgagcggggc
    15901 ctggcgctga cggcggtgcc gggctcgggg ccgggcggcg cggtgctggc ggcggacgtc
    15961 cccgacgccg caccggtgga ccgggatgcc ggcatgcgcg atgccatcgc ccgggcgatg
    16021 tcgcgctcga agcgcgagat cccgcactac tacctgcaga ccaccatcga cctcgggcga
    16081 gcgctcgact ggctgcggct ccggaacgag gagcgcccgg tcgtggagcg ggtgctcccg
    16141 gccgtgctgc tgctgaaggc gaccgcagtc gcggccgccg agcaccccgc cctcaacggg
    16201 tcgtgggagg acgggcaccg gcccgccgcg gacgtgcacc tcggcgtggc catcgccctg
    16261 cgtggcggtg gcctggtcgc acctgggatc gacgccgccg accgcatgtc cctcgccgag
    16321 ctgatggccg cgctgcggga tctcacccag cgcgcccgtg ccggacgcat ccgccagcgc
    16381 gagtggacgg gtccgacgat cacggtcacg aacctggggg acctcggggt cgacgccgtg
    16441 tggggggtga tccgaccccc gcagctggcg atggtcggct tcggtggcgt gcacgacgag
    16501 gtactggccg tcgacggtgc cccggccgtg cgcccggtgg tggtggcctc cctcgcggcc
    16561 gaccaccgga ccagcgacgg catcgagggc gcggcgttcc tgtcgcggat cgccgccttg
    16621 ctgcaggagc ccgatgccct cgatcgaacc gcaccccacc ccaccggagg agtcccgtca
    16681 tgaccgccct cacccaggcc gacgcccacg gcctcatcgc caagatcctc gccgacatcg
    16741 caccggaggt cgatctcgcc gacgcccgat ccgacggcca gctgcaggag gagctcggcc
    16801 tcgactcgat ggacttcctc aacctgatga tcggcatcca cgacgagacc ggcctcaccg
    16861 tgcccgagcg cgactacccg gagctcagca ccctcgacgg cgccgtccgc tacctgaccg
    16921 agcgcacagc ctgaccgccg tcaacgagcg gggcggggca cggcgaagcg gaacgtccgg
    16981 ctgacgagca gctcgtcctc ccacgtgcgg ccgctcagcg tcacctcgac gtggccgccg
    17041 ccgtcgtcgt cgacctccac gaggccgtac tgcccggccc cggggaacgc cccgtcggag
    17101 tacgggccgc cccggacgtt gccgggccgg tcgagcgcag ccgcgtgcag caccgggaag
    17161 ccgccgctgt tcgtgccgtc gtcgagcgcc accatgtgcg cgtcgccgct gaccatcacg
    17221 aggttctcga tcgatgcgtc ccggatcgcg gcgaggatct cctcccgctc ctcggggtac
    17281 ccaccccagt cgtcccggcc cggttcctcg gccgcgatcc acgggaccga gttgacccag
    17341 aggaccacgg catgggtccg cgacgagctg cggagctcgt cgaggagcca ccggagctgc
    17401 cggtcaccca gcatcgagtc cggggtcttc gcggagcggg tgtcggtcat caccacccgg
    17461 acccgcccga gcgtgaacgc ctggttgatc ggtccggtcg gtgccgcgac gttgggcacc
    17521 agctcccggt agccggtcct ggccgcgtcc cgactggggg agcggctgtc ggcgtcgttg
    17581 gggccgtagt cgtggtcgtc ccagacgtag gcgatcggga ccgctcggta cagcgcggcc
    17641 tgcgatgggg ccgtgaccac ccgctcgagc agggcccggt accggtcgag gtcgttcgtc
    17701 gccaggtcgc cgtagtggaa gtcgcccatg tggacgaaca ggtccgggtc ggcggcgcgg
    17761 atggcatcga acacctgccc gctcgatccg gtccgggcgc aggaggccac cgcgatgcgc
    17821 acgtcggtcg gtcccgaggc ggcggttcgg gtccggcccg acccgcgtga cccgtcgacc
    17881 cgaccgtcca cctccaccga ccaccggtgg tcggttccgg cagcgaggcc gtcggcctcg
    17941 aggcgcacga cgcgctcggc cgacacggcg accggctcgc tcctggtgac cgggccaccg
    18001 gcgtccgggg tgacgacgag gaccgcctcg tcgtggtccc cgtccaggcg ggcgaccacc
    18061 tggaagccat cggggcggac ccctccggac caggcccact cgacgaggtc gacgggaagc
    18121 gcgaccgtgt cggacaccgg gtgggccggg ccgtagaagt ggtcgtggac cgtggctgcc
    18181 gtcgcgtaca ccgacgtgcc cgacagcgcg cacaggacgg ccacgaccac gatcgctcgc
    18241 cgggttcgtc ctcgctgcca cgtgagccac agcccgatgg cggggatgcc gaacgacgcg
    18301 gcgacggcga ccgagacgag ggggtggtac gccaccgagg acagggcgcc gagcaccagg
    18361 ccgccgccgg cgaggccgat cgctccgagg agcgggtgcc ggagggacag ggcgagggcg
    18421 agggcgagca gggcgagcgt gccgagcgtg ccccagacct ccaccggagc ggcgaccgac
    18481 tcgccccggg ggttcctcgg gatgccgatc gtcagggcca ggatcccgaa ggtcgcgatc
    18541 gcgaccaggg cgacggcccg ggaggccaac ccgagcaggc cgtgggcgag gtcgggcagg
    18601 tggacgaacg tgtgggcggg ccccggcgtc ggtgaccacg ggcagccccg gcagcgggcg
    18661 tggagggcgg gacgggcgag cgcccagcgg accacgagca cgatggtcac gccgagcgcg
    18721 aggccgccca ggacgtcggt gggccagtgc agcccacgct gcacccgcac cagggcaccg
    18781 agcccgatgg cgactgcgag gagcacgccg gcgacacggg cgaggagcgc cgaccgggtg
    18841 aggacgagca gggccagggg caccagcccg gcgagcacca ccgactgcac catgtgaccg
    18901 ctggggtagg agtcctccag cccggtcatg atcgtgccgg gcgggcggtc gcgctcgatc
    18961 acctcgcgga ccagcgtggt ggccccgagg gaggcgacga cggcgccggc ataggcggcg
    19021 gcgaagggga ggcaccgcag ggtggccacg ccgatgagca gggccgcggc gacggcgacc
    19081 tcggtccgtc ccaggaggtg gacgccttcg agctgatcga cccactccca ggccctgacc
    19141 gtgtccgaca gccgctcgtc cgcgtcggcg aaggcgccgc gcgaggaccc gacgctcagg
    19201 gtgatcagcg cgaaggtggt gccgaccacc gtggccgacc acccgagcag gaaccggcgg
    19261 cgggacccgg cccgatcgct gtcgggcgcc tgtccgagcg gcgtggagat gagcagctcg
    19321 gtggcccagg ccggcgtgtc cgggtagcgg gcgtagagca ggccggcgcc gatcccgagc
    19381 cacagcagca ggaacccggt gatgaccgac agcgccagga gccaggcgat gtcggacacg
    19441 tagccgttgt cgcgcccgta gttgcccacc gcgccgatga ccacgtacat cgcggcgccg
    19501 atgcacgtga gggccgtgag gaggtacgcc gaccgcggcc gcagcacctg ctcgagcgcg
    19561 gcggtcgccg atgcccccgt cgtcacgtgg ccggccgtgg ctgcggcggc gatctcgacc
    19621 cggcgatccc ggtggcggtg ccgccggccg tgcacggcgt cccgcagcag cgtcacgatg
    19681 ctgaagagca ggccggcgga catgatcgcg gggacgaaga tgccgaaccg gtcgacgtcc
    19741 gcgtcggccc acgggtcgcg ggagctggtc atccgacagc gacctccgtg gggggcggcg
    19801 tgcgctcgtc ctcgtggacc gccacgatgc cggcgctgcc gtcgaccgtg accgtccggc
    19861 cgtcgaggtg gcgcgtcgcc ccgtgcacgt cgaggacggc gggcaggccc agctcgcggg
    19921 cgacgatcgc ggcgtgcgag agcggcccac cccgctcgac gaccaccgcg ccggcgtcga
    19981 tgaagagcgg cgaccacgcg gcgtcggtcg cccgggccac gatgacggag ccgggcggcg
    20041 gtggcgcgtc ggtcgggtcg cggacgacca cggcccgccc ggtgaagcga ccggggctcg
    20101 cggcgatccc cgtgaggcaa tcgccggtgg gcggctcgct cacggtcacc ggcggcatgc
    20161 cggtgaaccg ggacggcagc tcgtcctcct cccgccagcg cgccaggtgc cggcgacgtc
    20221 gaccgagcat cccgcggggc accgccgccc cgtcgagcag cgcccggcgc agctcgtcct
    20281 cgccgagcag ctccacgtcc tcggccgcgt cgagcagacc ccggtccacg aggcgggcac
    20341 ccgcctccag gtggatgagc cgcacgtgcc cgccgagcgc gagcaccgcg gccttgaccc
    20401 cctcccggcg ccccaggtcc tcgatggcgt cgccgatcgt gcgtcgcagc atgtggagcc
    20461 gcacgtccac gacctgcccc gagagcacgc gggtccgtcg ccagcgtgga tcggcgacca
    20521 ggatctcctc gaggtcggcc agtgcggagc cggcatcggg acgggctcgt gtgccgtgcc
    20581 ggtcgaggag ctggggggcc tcggaccagg tcggcccggc gaacaccgac cggctggcgg
    20641 tcggggcggg ggtgcggacc ccggtcgcac cgagggtgac ctcctgggtg tggcgggcgg
    20701 cgtcggcggc gtccaggtag cggcccagca cctgctccac gccggcgtag gccccggccg
    20761 cggcggcggc cacgccgagc tctgccgtca tggcccggac accgagatcg agcaggcgga
    20821 gtcgccgcga caggagctgg cggtcgtcga gccgggcgag cggagccgga tcggcgtcca
    20881 ccgctcgagc cacggcgatg accacgtcgc cgtcgaagcg cgagcgccgg cgcgtcgcca
    20941 tcgcccggag gtcccgacgg agcaggcgca tccacgacgc gcggggctgg accgcgggcg
    21001 gcgtcgttgc ctcggcgaag tactgggcct cgatgtcgga ggcagacgcg cccgggatct
    21061 gctcggccgc cgccttcagc aggtcgagat cgagcacggc ccgcccccgg acccgacgca
    21121 cgaaggcgtg ggggctggtc acgccgtcgg gaagcgcgtg cagcgagctg agcacgttcc
    21181 ggaacgcctc ctcgaccagg aacgaggcga ccccccaccg caggggcggc aggacgcccg
    21241 ggagcatctc gctgatcccc tccgaggtga gctcgtggtc gtccaccggc gtgtcgaagc
    21301 cgtcaccctc ggcggcgtcg acggtgatgg gtcgggactg caccagccac agccggcctc
    21361 cgtcccaggc ccactcgacg tcgagcggca cgccctccgc ggcttcgagc gccatcgccc
    21421 cgtcacggag ggcggcgaag tacccgggtg cgccggcggg gagcgacccg gatcgggcga
    21481 cgtgccacac gtcgggggtg cgctcgccgg acacgagggc ctcgccgagc ccggagacga
    21541 gctcgacgcg gaggtcgtcg gtcgggcggc cggggtcgac ggtgaagagc acgccggatc
    21601 gctcggacgg caccatctcc atgagcacca cggccatggc tgcgtcctcg tgcgggatgc
    21661 cccacgcccg gcggtaggcc cagggggcgg ggtgccacag cgacgcccac accaggcgga
    21721 tcgcgtccag caggtcgggg ccggtggtcg gcacgtcgag gagcgagcgg tactggccgg
    21781 cgaaggactg gtcgcgcagg tcctccgacg aggccgacga gcgcacggcg atgcggggtc
    21841 cgagcgccgg tgccatgctg cggatcgcgt cctcgagccc ctcgggcagc gggaccgcga
    21901 ggaaccgacg gtcgacctcc tcggccgtcg gtggctccgt cgccgtcgcc aacgcgtcga
    21961 ggtacgaccc gagctcggga tcggaggcga cccgccggta ctccgtgatc ggcaccaccg
    22021 ccgtgcgcgg caccgggtgg ccccagccga ccatgcgggc gagcgatgcc cccttgccgc
    22081 ccaggtcggc gccgtgctca ccgccatcgc cgaggaggac gacgcgcgag ctcatggccc
    22141 cgggcggtcc accacgtccg ctcgcaccgc agccacgcag cgatccacca cctcgtctgc
    22201 ggtctccccg gtcgccaccg cctgctcgga tgcccacgcc gcgagatccc gtcgcaccgt
    22261 caggccggcc cgtcccgtct gcgtccgcac caccaggagc agctgcagac ccacggcgag
    22321 ggcgaacacg acggcgaagt cccaggtcct gctgaccgtc gcgtccacca ggccgacgaa
    22381 ggacacgacc gtggccagca ccaggaccag gagtgcccgg ggtgaggaac caggcatggg
    22441 tgcatcgtgc gcccgcccgg tgcgcgctcc taggggagaa ggtcctccac ggacggcacg
    22501 atggatggac gttcggctct ggtgcccggg accgtgcggg ggtcccctgg gtgcagcgag
    22561 gacgacgacc gaggtgcgcc catggatgca ggtcccgacc gggagacggt gcttccgagc
    22621 acggtccggc tgcccgatgg tcgcgccgtc gtcgtccgca acgccggccc ggccgacgtg
    22681 cccggcctga tggcgctctt cgagcggttg ggccccgatg atcggtaccg gcggttcttc
    22741 tccgggttcc gtcccgacga cgacttcgtg cgcgccctcg tcatgcgccc accggaggag
    22801 ggccggctgc tcgtggccga ggtcacgggc ccggacggga ccgacatcgt cgccgaggcg
    22861 gagttcgccc gtctgtccga cggtgacggc gagctggccc tgacggtgga ccggggctgg
    22921 cgcggttggc tcgggccctt cctcctcgat gccctcctgc aggtcgccgc cgccgatgac
    22981 atcccggacc tccgggccga ggtgctcgcc cagaaccggc cgatgctcgc cctcctgcgc
    23041 gcccgcggct gcgccatggt ggagagcggg gacccctgcg tcacggaggt cgtcgtgggg
    23101 acccacggcg cggcgccctc gtgggcaccg gggtcggcgc acccccgggt cctgctcgaa
    23161 gcggcgggtg ggcggtggca gctgacgacg aagctcggcg aggccggcgt gccggtgctg
    23221 cgctgccccg ggccgggcgt gcggcctgcg aacgtgccgt gccccctcct gcagggggaa
    23281 cggtgcccgc tggcggacgg tgccgacgtc atcgtccacg ccctcgaccc ggatgacccc
    23341 cgcagcgtca ccgtgctcga cgcccaccgc cgggcgggtg gtcgcgtcgt ggaggtgggg
    23401 cccccgggca cggacgacgt cgacgcgatc gtcgcggagg tcgtgcgagc tcccacgcgg
    23461 gcgccggacg gaggctgacg tgctcttccc cacgatccgc ttcgccctgt tcttcatggt
    23521 gacgtggggc gtgctgctcg gcgtgcggga tcgccccatc gcccgccggg gcgtgctgct
    23581 cggcgcgagc tgggtcttct acgccgcgtg ggactggcgg tggctggcgc tgctgtgggc
    23641 gatgatcggc gtcaaccacc tcgccgtccg cggggtcgcc tgggcgccga ctgccgctgg
    23701 tcggcgcgcc gcgctcgcgc tgggcgtggg cgccgacctg gcgctgctcg gcaccttcaa
    23761 gtactacgac ttcttcgccg agtccatggt cggcaccctg ggcctgctcg gcgtgcaggc
    23821 ctccgcgccg ttgctgcagg tcgccctccc gctcgggatc tcgttcctca cgttccaggc
    23881 gatcagctac ctcgtggagg tgcaccgggg cgtcaccgcg cctgccccgc tcctcgacga
    23941 ggcggtctgg ctgagcttct tcgccaccgt ggtgtccggc ccgatcaccc gggccagcga
    24001 gctcatcccc cagctcgacc gggtgccgag ggccgtcgac cccggcgcgt cgttcgccct
    24061 cgtcatccgg ggcctgttca agaaggtcgt gctggccagc tacctggcca ccgccgtcgt
    24121 ggacgaggcc ttcgccacgc ccgcagcgtt ctccgccacc gaggtcctgt tcgccgtcta
    24181 cgcctacggc gccctgatct acgtcgactt cagcggatac accgacatcg cccgcggcgt
    24241 cgccggactg ctcgggttcc acctgccgga gaacttcgac gccccctacc gcgccgccag
    24301 cgtgcaggag ttctggaccc ggtggcacat gacgctcacc cggtggctgc gggacttcct
    24361 cttcaccccc ctcgggctcc gctacgggcg acggcgcgtc atcgggccgc tgctcccgct
    24421 cctgatcatg ctggcggcgg gcctgtggca cggcgcggcc tggaccttcg tggtgttcgg
    24481 cggcatccac ggcctcgccc tcgtcgccga gcgggccgac cgcgtgcgcc gccgccggct
    24541 cgggctcggc cgggccccgg acaccccggg gcgccgtctc gtgcgccacg tcgtcaccct
    24601 ccaggtcgtg tcgctcgcct ggttgttctt ccgggccgag tcgatgggag cggcgtgggc
    24661 gatgctgcac cgcatcgcga ccggaccgct cgcgacggga caggtcaccc tgctggcggt
    24721 ggcgaccgtc gtcggcatct acggcgtcca gctcgtccgt gccccggccg tcgccgagcg
    24781 ggcccaggcc tgggcgacgc acgccgggcc ggtcacccag gccgtcgggc tggccggcgc
    24841 cctcaccgtc gtggacgtgc tcggtcccgc cggcgtcgcc cccttcatct acttcgggtt
    24901 ctgatggcgc tgacgacccc cgccactgcg acgttccctc ccgacgagct gccggccggt
    24961 ccgtcacccc ggtcgggtcg ggtgtggcgt cccttcgccg tcgctgccgt ggcgctcgtg
    25021 ctcgccgcgc tgctcaacgc agacgagctg cggcagaccg ccgagcgcca gccggtcggg
    25081 tgggagcgag aggcgctggt cgatctcgct gcgatcccca aggaggtcgc cgacgccacc
    25141 cggctggatc gcccgcgagc gtgggcggag tcgctgctcg gcacgcagga accggccgcc
    25201 atcgcagtcg gtcctccgga accaccgccg gcccggccac ccgacacgca ggtcccggtc
    25261 accgacccgg ccggctcgtc cacctcgacg tccaccacca cgaccgtccc ggagcgccgc
    25321 acgccgacgg cgggcgcacc gctgcaggtg gccgtgatcg gggactcgat gatgcaggtc
    25381 ctcgggcagt cgatcgtcaa cggagcggcc ggcgacgatc gcctcgagac gagcctcgag
    25441 taccgggtgt ccacggggct gacccgaccc gactacttcg actggccggg tcgcatcacc
    25501 gagctgctcg cggccggccc gctcgacgca gtggtggtca tgtacggggc gaacgacgcc
    25561 cagggcatgg tcctgccgac cgggtccgag cccttcggca cccccgtctg gctcgacgag
    25621 taccggcgtc gggtcggctg ggtcatggac gccctcgtcg ccgggggagc ggagacctac
    25681 tgggtgggcc agccggtcat gcgatcggac tcgttctcgg cccggatggc gtcgttgaac
    25741 gaggtctacg catcggaggc ggcgctgcgg gaccgcgtga cctacgtgga cgcctgggcg
    25801 gtcctctcgg tggacggtgc ctacacatcg cacatcgacg acggtggcgg cccgacctcg
    25861 gttcgccaga gcgacgggat ccacctctcc cgcgccggcg gcgaccggct cgccgccgtc
    25921 gtgctcgacc agatccgcag cgactggagg ctcgacccat gagacccctt cgaacgatcg
    25981 gggtggtggt gacctgcctc gccctcgtcg ccgggtgcgg cgggtcgagc acgcagaccg
    26041 cgaccgtccg gtcgaccacc acggccacga cctccacgag ctcgaccacg gtcgcgccga
    26101 gcacgaccgc gcagccggtc ccgtccacga cgacgtcggc acccaccacg gtcgcgccga
    26161 gcacgaccgc accgaccacg accccaccga cgacggcgcg tcctccgtcg cccacgggtc
    26221 gctgggtgtt cgtgctcggc gactccgccc tgctcggtgc ccgggactcg gtgccggccg
    26281 ccctccccgg ttggcaggtc gtcttcgatg ccgtcggcag caggcggctc ccgcaggcca
    26341 tcgaggtgct gcgagcccgg cggtcggaga tcggttccgt cgccgtcatc cagcagggga
    26401 acaactacct cgagagcgag gggtccttcg gtgcgcagat cgacgaggcg atgcgggtgc
    26461 tcgacggcgt cgaccgggtc gtctggctga cggtggcgga gaagtggccg tcccgggtcg
    26521 agatcaacca gcacatcaga gcggccgccg gtcgctggcc ccagatcgtc gtggccgagt
    26581 gggcgcccct catcgccgcc cacccggagt acaccgagga catgctccac ctgtccgccg
    26641 ccggtcgcgt cgccatcgcc gagctcatcg ccggcgcggt caccgcgtag gcgacggcgg
    26701 agggcagctc gtcagaactt ggagtcggtg gggaggtgcc cggcggcgac ctggtcgtcg
    26761 gggacccggc gccggtactg ctcgttctcg atgatggagc tcttcgtgag ggcgaggccg
    26821 ccggccatga cggcgccgta gacggtgatg gcgaagacca cggcaccgac gacgaagagc
    26881 acggtctcgc tcatgaccgc accgcgtcgg ccaggtcacg ggcgtcggca tgcaccggcg
    26941 tgagcaggag ggtgccgagg gagtggatcg agggcaccca gaggccgacg aagatgccgg
    27001 cggtggtctc gtcggcgacg aagtagaggt acacggagaa gacgaacgac gcgaagccgg
    27061 cgacgaggaa cggcagccga acgacgaggt gccgggg
//
LOCUS       DATISU010000011         1510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_606080, whole genome shotgun sequence.
ACCESSION   DATISU010000011 DATISU010000000
VERSION     DATISU010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606080"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VLR27_00665"
     CDS             <1..507
                     /locus_tag="VLR27_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HSP01981.1"
                     /translation="ARAMEQSGKVGIARVVMRTKQYLCAVRPQDGKLVMSTMVYADEV
                     NDPAQIPELAQLDDVEVSDKELVMAEQLIASLSETFDPAKYTDTYRTKVLEVIERKAA
                     GEEDVIATPEPQEEKVVDLMAALEASVAAAKEARGRHPTAATPGTVSGEEIAGEDAPA
                     DEDAAQTA"
     gene            504..1397
                     /gene="ligD"
                     /locus_tag="VLR27_00670"
     CDS             504..1397
                     /gene="ligD"
                     /locus_tag="VLR27_00670"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HSP01982.1"
                     /translation="MTERLVELDGQRLKLTNLEKVLYPETGFTKAEVIDYYARIAPVM
                     LPHIAGRGITLKRFPDGVEGGSFFEKRCASHRPEFVDTIDGPGDRGGAIGYCNLDSIP
                     ALAWAGNMAALEIHAPMALGADIESPQLCVFDLDPGEPATIIECARVGLELRDVLAGI
                     GLDCVAKTSGSKGMQLYVPLNSPHTHDHTSEFAHAVARVLEKHRPDDVTSVMAKAQRP
                     GKVFIDWSQNSRHKTTVAAYSLRAKPHPTVSTPVSWDEVSDCADGQPLSFTAPEVLTR
                     VADLGDLFAPCATQVQHLPPT"
BASE COUNT          259 a          557 c          497 g          197 t
ORIGIN      
        1 gcccgggcca tggagcagtc gggcaaggtc ggcatcgccc gggtggtgat gcgcaccaag
       61 cagtacctgt gcgcggtgcg gccccaggac ggcaagctcg tgatgtcgac gatggtctac
      121 gccgacgagg tgaacgaccc ggcccagatc cccgagctcg cccagctcga cgacgtcgag
      181 gtctccgaca aggagctggt gatggccgag cagctcatcg cctccctcag cgagaccttc
      241 gatccggcca agtacaccga cacctaccgc accaaggtgc tcgaggtcat cgagcgcaag
      301 gccgccggcg aggaggacgt gatcgccacg cccgagccgc aggaggagaa ggtcgtcgac
      361 ctcatggccg ccctcgaggc atcggtcgct gcggccaagg aggcccgcgg acgccatccc
      421 acggcggcca cccccgggac cgtctccggt gaggagatcg ccggcgagga cgcacccgcc
      481 gacgaggacg ctgcgcagac cgcgtgaccg agcggctcgt cgagctggac ggccagcgcc
      541 tgaagctcac caacctcgag aaggtgctgt acccggagac cgggttcacc aaggccgagg
      601 tcatcgacta ctacgcccgc atcgcgccgg tgatgctgcc ccacatcgcc gggcggggca
      661 tcacgctgaa gcgcttcccc gacggcgtcg aggggggttc gttcttcgag aagcgatgcg
      721 cctcgcaccg accggagttc gtcgacacga tcgacgggcc cggcgaccgg ggtggagcga
      781 tcgggtactg caacctggat tcgatcccgg cgctggcgtg ggcggggaac atggctgcgc
      841 tcgagatcca cgccccgatg gccctcggtg ccgacatcga gtcaccgcag ctgtgcgtgt
      901 tcgacctcga ccccggtgag ccggcgacca tcatcgagtg cgcccgggtc ggtctggagc
      961 tgcgcgacgt gctcgccggc atcggcctcg actgcgtggc caagacgtcg ggctccaagg
     1021 gcatgcagct ctacgtgccc ctcaactcgc cccacaccca cgaccacacc agcgagttcg
     1081 cccacgccgt cgcccgggtc ctcgagaagc accgtcccga cgacgtcacc tcggtgatgg
     1141 ccaaggccca gcgcccgggc aaggtcttca tcgactggag ccagaacagt cgccacaaga
     1201 cgacggtggc cgcatactcg ctgcgagcca agccccaccc gacggtgtcg acacccgtga
     1261 gctgggacga ggtcagcgac tgcgccgacg gccagccgct ctccttcacc gcccccgagg
     1321 tgctgacccg cgtcgccgac ctcggcgacc tcttcgcccc ctgcgccacc caggtccagc
     1381 acctcccccc gacctaaacg cggggtgggg gaggaggcgg aagaaactgg cagccccctc
     1441 gtcacaccgg cgacgtgcac gcatcgtccg agctacgttg ccgtcggctg cgcgacggcc
     1501 gacttccccc
//
LOCUS       DATISU010000012        11520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_792377, whole genome shotgun sequence.
ACCESSION   DATISU010000012 DATISU010000000
VERSION     DATISU010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 11520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11520
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792377"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VLR27_00675"
     CDS             complement(<1..292)
                     /locus_tag="VLR27_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HSP01983.1"
                     /translation="MSLPRVIAVAPGSPAARAGLVEGDEILSLDGVQPRDVIEWHLLV
                     DEPEVVLDVRRGGLEVQVTVSKGSGEPLGADVDAAIFDEVQTCDNHCEFCFIY"
     gene            complement(352..1992)
                     /locus_tag="VLR27_00680"
     CDS             complement(352..1992)
                     /locus_tag="VLR27_00680"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HSP01984.1"
                     /translation="MPGTMTTRLARALLVALLACTGLATGASPASAAACTDPFTPDLH
                     AAIARDFPGQRVTAAVHDVRTGCWSHLAPGTVMTTASVIKAQFLAGVLLRAQDEGRGL
                     TAWERDRVIPMMERSHNPPASDLYVSLGGDAGQQRIDQRFGLTNTTSTSRWGLTASTA
                     EDRTRLATGLLHGGGPLAAPGRDEAWRIMTAVHPTQQWGISAGVPTGWTVALKNGFYP
                     TAGAARWRIGSTGFVRHDATGSGYAITVMTDQNPDHATGQRLVELVSRAVAGSLTTGE
                     AAPRPVDRSVCVATTTGESWAQVAGRLGTGDVAGVRSVSGGPDQPLSGMRACRPDLRV
                     EYDPDSAVAHHVRASFDLFLGRAPSADELEWHTVAIESGSISRLGFARSLAASQEWID
                     ATIDALYRSALGRPAEEAGLAHWRARVAAGMPITDLVALIHGSDERYATVGGTDDAFV
                     RGLYRDLLHREPDGAGLQHWIGQLRAGVGRTTAVASFHGSLESRRIRVTAAYQRVLSR
                     PPDAGGLRHWAQVLSRLDDVALDAHLAASGEFFRSAQP"
     gene            2068..3237
                     /locus_tag="VLR27_00685"
     CDS             2068..3237
                     /locus_tag="VLR27_00685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP01985.1"
                     /translation="MELTIESIRSGDRERHHALMRQAFGGTDAFDPSAPQTDADKFVC
                     AYLGDQLVGSVLTFDFAMTWGGRRVRCGGVSGVVVSPQARGRGAAQRMLDESLRRMHD
                     QGQVLSALYPTTASLYRRAGYEVVGHYRRTRIPMAAIQPTGEALEWREVDPSSEVLSA
                     LHDDLAGRLDGWFRVDPQWWAFRSHRQAADTSTNRFTYVGARAGSDVAAVQYRYAKAD
                     DFYDLEVEVLGGLDVEAVGAALGLLAGHGTTARNVVVALPTSLLGPHVPQLQLTRSES
                     DWPWMLRLVDAPGAVAARGWPTSVSGSVELDVIDDRCPGNAGPHVLEIGGGEAGLLRG
                     GSGRIRVTSQDLAMVYSGCDVPGLRAAGRLVEASAADLDLLAAACVSNPSIPLFF"
     gene            3475..4206
                     /locus_tag="VLR27_00690"
     CDS             3475..4206
                     /locus_tag="VLR27_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01986.1"
                     /translation="MPSSPLRRRARLLLSATLAAAIAVTGLAAAPAGAQTIEPAAEAG
                     VIDPDTRFDSALATLLRVIYRAENGGADVEPTPEDLEAKAKVDCVRAGPADADVPTMV
                     AEIFRCRLAEAGFDQKRIDNWTADALVVSHCESLWDTTAVVFNGRYLDSPHPNGNRYS
                     AAGVFQFIRATADKWIEGGYANVHDARLNIDAAARLFIHNRTLGLRGWEDWACAAAHD
                     GFKVGSVLPGWPGGPAELPAWAWDH"
     gene            4244..5284
                     /gene="ispH"
                     /locus_tag="VLR27_00695"
     CDS             4244..5284
                     /gene="ispH"
                     /locus_tag="VLR27_00695"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HSP01987.1"
                     /translation="MPVEQVLLAAPRGFCAGVEMAIKALAWMVRAFEPPVYCYHEIVH
                     NQLVVQRFRDLGVVFVDDIDEVPPGRPLMLSAHGSAPEVVAAAQASGGYVVDAVCPLV
                     TKVHHEVKVRAGKGYQIVYVGHEGHEEAVGTMAVAPDAIHRVESTDEVDALPHFDQPV
                     ALLAQTTLSHRDWEGVLDRTRERFPDVWTPGRSDLCFATTNRQAALTALAERCDAIVV
                     IGSANSSNTRALESLARDMGCERVHRVNTPEEVPDDLSGVVGVTAGASAPEELVCWVI
                     DKLDPANGIEEIRITEEEEYFPPPRNLRELLDALDVVATLMVGGTLDDRPTVVDRETK
                     ASEVLGALVVGP"
     gene            complement(5281..5808)
                     /locus_tag="VLR27_00700"
     CDS             complement(5281..5808)
                     /locus_tag="VLR27_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSP01988.1"
                     /translation="MDRRRFLQVSAVTVAGAGLGACGDDDSGPEIAEEGQQLGTPDEV
                     AAAIEEGGGAWYIPEAQTYVVPVPEEHREALAAAVPESIRPGIEGGLIALWQKCPHQG
                     CRVPWCESSGWFECPCHASRFSPFGELRRGPARRGMSYLPITLKPDAVRLLPGPVDGL
                     EDDITQIDAAGPHCV"
     gene            complement(5820..6536)
                     /locus_tag="VLR27_00705"
     CDS             complement(5820..6536)
                     /locus_tag="VLR27_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSP01989.1"
                     /translation="MAEPVPAADPRGNPVEEQGPPTKGQLRFYRAVTAVIVGFCRLFW
                     RLEVHGLDKVPDGPFVLSAVHRSNVDSLLVAAVARRRMRFMGKHTMWKYDLPGRFFDA
                     MGGIPVRRGSADREAMRSAVTALGYGEPVVMFPEGQRRTGPVVEDLFEGPAFVAGRTG
                     VPIVPVGIGGSEAAMPPGAKWIKPVKVVLVIGDPIPAPVGEDGKRPSRRQVRETTALL
                     QTRIQALFDDAQRRVGRLGD"
     gene            complement(6529..7179)
                     /gene="cmk"
                     /locus_tag="VLR27_00710"
     CDS             complement(6529..7179)
                     /gene="cmk"
                     /locus_tag="VLR27_00710"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HSP01990.1"
                     /translation="MSNDGLRVIAIDGPAGSGKSTVARLLAERLGLEYLDTGAMYRAV
                     TFAALRRNLDPADAEPVAALAAKVDLAVTLDGVVVDGVDATIEIRGPEVTRAVSTVAA
                     NPGVRAEMVSRQREWARRRGGGVLEGRDIGSVVFPDAALKVYLDARPEVRAARRSKEV
                     SDLDYETVAADIARRDALDQGRDASPLTRAGDAVEVDTSDLTIDEIVELLAEQIDG"
     gene            complement(7190..8488)
                     /gene="aroA"
                     /locus_tag="VLR27_00715"
     CDS             complement(7190..8488)
                     /gene="aroA"
                     /locus_tag="VLR27_00715"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HSP01991.1"
                     /translation="MPDPLLIVPVDTPIDATVTLPGSKSITNRALVCAALADGTSVLT
                     GVLYADDTEAMVEALRTLGVEVTGTGHAELTVVGSTERRAGAVDARQSGTTARFLLPL
                     LALGEGMSTLDGDVQLRDRPMGPTFDALRSAGVRVEEQAGAGRLPVTVAGPLRTGSLA
                     VPGDVSSQFLSGLLLSGPATPDGLELTVTTELISRPYVDMTVAVMEAFGARVDHDEGG
                     GRETWHVAPEPYRPTTYAIEPDASAASYAWAAALVTGGRVTVPGLHRGSLQGDVDFAD
                     VLGQMGADVTWHDDAVTVAAGDHLHGVDVDMAHISDTVPTLAALAALADSPTTIRGVG
                     FIRDKESDRIATPVAELQWCGIDAAATDDGMVIRPGPLHSATFDTYRDHRMAMAFGLL
                     GLVVPGMAVRDPGCVAKTFPGYWDLLDDLRAGSVAPSRQR"
     gene            complement(8491..9561)
                     /locus_tag="VLR27_00720"
     CDS             complement(8491..9561)
                     /locus_tag="VLR27_00720"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HSP01992.1"
                     /translation="MPGASQPRANVVGVGLIGGSIALALRAEGWHVSGVDHDEARTDR
                     ARELDVIDQVGLDADADITFVATPVRTVAEAARKALAGTSGLVTDVGSVKAPIVAAVD
                     DVRFVGGHPMAGSEQEGVDGATADLFEGAVWVLTPTADTDDAAYARIRGIVSTFGAEV
                     IALPPDRHDELVAVVSHVPHLTAASLMRIADERADEHAALLRLAAGGFRDMTRIASGH
                     PGIWPDICEENQVAIVAGIDRLIAALAEARDQVASSDRDALLADLTRARAARVNLPSR
                     YTRPSELAEVRVPVPDRTGVLADVTTLATELDVNIVDLEIAHSSEGQQGVLIVLIEGA
                     LVERFRGGLLARGYRPSIQHLG"
     gene            complement(9573..10298)
                     /locus_tag="VLR27_00725"
     CDS             complement(9573..10298)
                     /locus_tag="VLR27_00725"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HSP01993.1"
                     /translation="MSEPERLQKILARVGIASRRVVEEMIEDGRVVVNGEVAELGQRA
                     DPDVDAIEVDGVLVGVRPDTVWYLLNKPAGVVSTASDPQGRPTVVQLVPEEPRVFPVG
                     RLDAETEGLLLLTNDGEVTHRLTHPSYGVEKEYLAHVEGTPSRGALRTLREGVELEDG
                     ITAPATAAMVSPGVVRLVIHEGRNRQVRRMCEAVGHPVVRLVRTRIGPLVDTKLGPGE
                     WRPLRQDEVRALERAAVPTAPPT"
     gene            complement(10295..10975)
                     /gene="scpB"
                     /locus_tag="VLR27_00730"
     CDS             complement(10295..10975)
                     /gene="scpB"
                     /locus_tag="VLR27_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HSP01994.1"
                     /translation="MTEPEPTAGEDDRERELRRAAEALLLVTEEPITVELLAQLLEVS
                     TDRVEALCVELAAEYDATDRGFQLVKVAGGWRYQTHPDLAPYVERYVLDGQSGRLSNA
                     ALETLAIVAYKQPMSRAQIAAIRGVNVDGVMRTLVQRGYVDEVGRDHGPGQAVLYGTT
                     PTFLEKLGLNSLDELPALGEFVPGADVYEALEHGLRPDPLDADTTAPVDGDGDGEHAS
                     DAADDESE"
     gene            complement(10978..>11520)
                     /locus_tag="VLR27_00735"
     CDS             complement(10978..>11520)
                     /locus_tag="VLR27_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HSP01995.1"
                     /translation="DDELALWEERDLLLSRLVECKTFKDAAAAMAAIANEAGRSYPRL
                     AGVDEQFLGLLPDLLATTTPDQLRDAFLRAVEPKPVPRVHIDHIHQIRVTVTDAVAEL
                     LDELPRIGRATFAELTADLVERLEVVVRFLAVLELYKQGWIDLQQVNSLGSLTVVWTG
                     RPDDDELDLDDLAFVDAYDG"
BASE COUNT         1553 a         4170 c         4251 g         1546 t
ORIGIN      
        1 ggtagatgaa gcagaactcg cagtggttgt cacacgtctg gacctcgtcg aagatcgcag
       61 cgtcgacgtc tgcgccgagg ggctccccgg accccttcga cacggtgacc tggacctcga
      121 gcccgccacg gcgcacgtcg aggaccacct ccggctcgtc gaccaggagg tgccactcga
      181 tgacgtcgcg cggctggacc ccgtcgaggg agaggatctc gtctccttcg acgagacccg
      241 ctcgggctgc gggtgatccg ggcgcgaccg cgatgacgcg gggcaggctc accggcgaca
      301 gggtaccgcc ggaacggccc ggaaccccag gtcgcggact accggcaccc ctcagggctg
      361 cgcggagcgg aagaactccc cgctggccgc gaggtgcgcg tcgagggcga cgtcgtcgag
      421 ccggctgagc acctgggccc agtggcgcag gccaccggca tccggaggcc gcgacaagac
      481 ccgctggtag gcggccgtga cccggatccg gcgggactcg agcgaaccgt ggaaggaggc
      541 caccgcggtc gtgcgaccca cgccggctcg gagctggccg atccagtgct ggaggccggc
      601 gccgtcgggc tcccggtgca gcaggtcccg gtagaggccg cggacgaagg cgtcgtcggt
      661 gccaccgacc gtggcgtagc gctcgtcgga gccgtggatg agcgccacca ggtcggtgat
      721 cggcatgccg gcggcgaccc gggcgcgcca gtgcgccagc ccggcctcct cggccggccg
      781 gccgagggcg ctgcggtaca gcgcatcgat cgtggcatcg atccactcct gcgacgccgc
      841 cagggatcgg gcgaagccca gccggctgat cgagccggac tcgatggcga ccgtgtgcca
      901 ctcgagctcg tccgcggacg gtgcccggcc gaggaacagg tcgaacgacg cgcggacgtg
      961 gtgggcgacg gcgctgtccg ggtcgtactc gacgcgcagg tccgggcggc acgcccgcat
     1021 ccccgacagc ggttggtccg gtcccccgga caccgaacgg acgccggcca cgtccccggt
     1081 gccgaggcgc ccggccacct gggcccagga ctcccccgtc gtcgtggcca cgcacaccga
     1141 gcggtcgacg ggtcgcggtg cggcctcgcc ggtggtcagc gacccggcca cggcccggct
     1201 gaccagctcg accaggcgct gcccggtggc gtggtccggg ttctggtcgg tcatgaccgt
     1261 gatcgcgtag ccggagccgg tggcgtcgtg ccggacgaag cccgtggacc cgatgcgcca
     1321 gcgcgccgct cccgccgtcg ggtagaagcc gttcttgagc gccacggtcc agccggtcgg
     1381 gacgcccgcg gagatgcccc actgctgggt cgggtgcacg gccgtcatga tccgccacgc
     1441 ctcgtcccgg ccgggggcgg ccagcggccc gccgccgtgg agcagtccgg tggcgaggcg
     1501 ggtgcggtcc tcggccgtgg acgcagtgag cccccaccgg gacgtgctcg tggtgttcgt
     1561 cagcccgaag cgctggtcga tgcgctgctg tcccgcgtcg ccgccgaggg agacgtagag
     1621 gtcgctggcc ggcgggttgt gcgagcgctc catcatcggg atgacccggt cgcgctccca
     1681 cgcggtgagc ccgcggcctt cgtcctgggc ccggagcagg acgccagcga ggaactgggc
     1741 cttgatgacc gacgccgtgg tcatgaccgt gcccggcgcc aggtgggacc agcagcccgt
     1801 ccgcacgtcg tgcacggcgg cggtgacccg ctgaccgggg aagtcccggg cgatggcggc
     1861 gtgcaggtcc ggggtgaagg ggtcggtgca ggcggcagcc gacgcaggcg acgccccggt
     1921 cgccagcccg gtgcaggcga gcagcgcgac gagcagcgcc cgcgcgagac gcgtggtcat
     1981 ggtccccggc atggcgcggg atcgtagcga tggcccgtcg tgaactcgtc cgaccccacg
     2041 gtcgccaccg acgtaggttc cgcgtccatg gagctcacga tcgagtcgat ccggtccggc
     2101 gaccgggagc ggcaccacgc cctcatgcgc caggcgttcg ggggcacgga cgcgttcgac
     2161 ccgtcggcgc cgcagaccga cgcggacaag ttcgtctgcg cgtacctcgg cgatcagctg
     2221 gtcggatcgg tgctcacgtt cgacttcgcc atgacctggg gcggacgccg ggtccgctgc
     2281 ggtggggtgt ccggcgtcgt cgtgagcccc caggcgcgag ggcgcggagc agcgcagcgg
     2341 atgctggacg agtcgcttcg ccgcatgcac gaccagggac aggtcctgag cgccctgtac
     2401 cccaccaccg cctccctgta ccgccgcgcc ggctacgagg tggtgggcca ctaccggcgg
     2461 acccgcatcc cgatggccgc catccaaccc acgggcgagg ccctggagtg gcgcgaggtg
     2521 gacccgtcga gcgaggtcct ctccgccctc cacgacgacc tggccggtcg cctcgacggg
     2581 tggttccggg tcgacccgca gtggtgggcg ttccgcagcc accgccaggc tgcagacacc
     2641 tccaccaacc gcttcaccta cgtcggcgcc cgagccgggt cggacgtggc tgccgtgcag
     2701 taccgctacg ccaaggccga cgacttctac gacctcgagg tcgaggtgct gggcggactg
     2761 gacgtggagg ccgtcggcgc cgccctcggg ctgctggccg ggcacggcac caccgcccgc
     2821 aacgtcgtgg tcgccctgcc cacctccctc ctcggccccc acgtgcccca gctccagctg
     2881 acccgcagcg agtcggactg gccctggatg ctgcgcctcg tcgacgcacc cggcgccgtc
     2941 gccgcccggg gctggccgac cagtgtctcg gggtcggtgg agctggacgt gatcgacgac
     3001 cgctgcccgg gcaacgcggg cccccacgtg ctcgagatcg gtggtggtga ggccggactc
     3061 ctgcggggcg ggtcgggccg gatccgggtc accagccagg acctggccat ggtgtactcc
     3121 ggatgcgacg tgccgggtct gcgggccgcg ggtcgcctgg tcgaggcgtc ggccgccgac
     3181 ctcgacctgc tggcagccgc ctgcgtgtcg aacccctcca tcccgctgtt cttctgacct
     3241 cgggaccttc gaccctcgga acggtctcac ggagcggtct ggaggcgatc tgacgcgctt
     3301 tcgccactct gcatgggtcc atcgtcactt tgcgatcaga tgacgaggga ggcgcccgca
     3361 ggtgcgcttg actgatgtcc agcgccaagg agcgccgctg cgaaccgcag cgatcagacc
     3421 acctccgccg tcttccacgc ccccgagcgg gccgtccccc cggagaagtc ctcgatgccc
     3481 tcgagccccc tgcgccgccg cgcgcgcctg ctgctgagcg ccaccctggc cgccgccatc
     3541 gccgtgaccg gtctggccgc cgcacccgcg ggcgcccaga ccatcgagcc ggccgccgag
     3601 gccggcgtga tcgaccccga cacccgcttc gactccgccc tcgccaccct gctccgggtg
     3661 atctaccggg ccgagaacgg tggcgccgac gtggagccga cccccgagga cctcgaggcg
     3721 aaggcgaagg tcgactgcgt ccgcgccggt ccggccgacg ccgacgtccc gacgatggtc
     3781 gccgagatct tccggtgccg cctggccgag gccgggttcg accagaagcg catcgacaac
     3841 tggaccgcgg acgccctcgt cgtctcccac tgcgagtcgc tctgggacac cacggccgtc
     3901 gtgttcaacg gccggtacct cgacagcccc caccccaacg gcaaccgcta ctccgccgcc
     3961 ggcgtgttcc agttcatccg ggccacggcg gacaagtgga tcgagggcgg ctacgccaac
     4021 gtccacgacg cccggctgaa catcgacgct gccgctcgcc tgttcatcca caaccggacc
     4081 ctcggcctgc ggggctggga ggactgggcc tgcgccgccg cccacgacgg gttcaaggtc
     4141 ggttccgtgc tccccgggtg gcccggcggc cccgccgagc tgcccgcctg ggcctgggac
     4201 cactgatccc agcgatgtga gcagacgtcg gtagcgtgga gtcgtgcccg tcgaacaggt
     4261 cctcctggct gcaccccgtg gcttctgcgc cggcgtggag atggccatca aggccctcgc
     4321 ctggatggtc cgggcgttcg agccgccggt gtactgctac cacgagatcg tccacaacca
     4381 gctcgtggtg cagcgcttcc gggacctcgg cgtcgtgttc gtggacgaca tcgacgaggt
     4441 cccgccgggg cgtccgctca tgctctcggc ccacggctcg gctcccgagg tggtcgccgc
     4501 cgcacaggcc agcggcggct acgtcgtcga cgccgtctgc cccctcgtga ccaaggtgca
     4561 ccacgaggtg aaggtgcgag ccggcaaggg ctaccagatc gtctacgtgg gccacgaggg
     4621 ccacgaggag gcggtcggca ccatggccgt ggcgcccgac gcgatccacc gggtcgagtc
     4681 caccgacgag gtcgacgccc tcccccactt cgaccagccc gtcgccctcc tcgcccagac
     4741 caccctgtcg caccgcgact gggagggcgt cctcgaccgc acccgagagc gcttccccga
     4801 cgtgtggacc ccgggtcgca gcgacctctg cttcgccacc accaaccgcc aggcggccct
     4861 gacggcgctg gccgagcgct gcgacgccat cgtcgtgatc ggttcggcga actcgtccaa
     4921 cacccgcgcc ctcgagtcgc tggcccggga catgggctgc gagcgggtgc accgcgtcaa
     4981 cacgcccgag gaggtccccg acgacctctc cggcgtggtc ggggtcaccg ccggcgccag
     5041 cgcaccggag gagctggtgt gctgggtcat cgacaagctc gacccggcca acggcatcga
     5101 ggagatccgc atcaccgagg aggaggagta cttccccccg ccccggaacc tgcgcgagct
     5161 gctcgacgcg ctcgacgtgg tcgccacctt gatggtcggc ggcacgctgg acgaccggcc
     5221 caccgtggtc gatcgcgaga ccaaggccag cgaggtcctc ggcgccctgg tcgtcggccc
     5281 ctagacgcag tggggaccgg cggcgtcgat ctgggtgatg tcgtcctcga gaccgtccac
     5341 cggaccgggc aggagccgca cggcgtcggg cttgagggtg atcggcaggt agctcatccc
     5401 ccggcgggcc ggcccacggc gcagctcgcc gaacgggctg aaccgggagg cgtggcacgg
     5461 gcactcgaac cagccggacg actcgcacca cggcacccgg cagccctggt gcgggcactt
     5521 ctgccacagc gcgatcaggc cgccctcgat tccagggcgg atcgattcgg ggacggcagc
     5581 ggcgagcgcc tcgcggtgct cctcgggcac cgggaccacg taggtctggg cctcggggat
     5641 gtaccaggca ccgccacctt cctcgatcgc tgcggcgacc tcgtcggggg tgccgagctg
     5701 ctggccctcc tcggcgatct ccggcccgct gtcgtcgtcc ccgcacgcgc cgagacccgc
     5761 gccagcgacg gtgaccgcgc tcacctgcag gaagcgtcgt cgatccatgg gtcgtctcct
     5821 cagtcgccga ggcgcccgac gcgacgctgc gcgtcgtcga acagcgcctg gatgcgggtc
     5881 tggagcagcg cggtggtctc ccgcacctgc cggcgcgacg gtcgcttgcc gtcctcgccg
     5941 accggtgccg ggatcggatc gccgatcacc agcaccacct tcaccggctt gatccacttc
     6001 gcgccggggg gcatcgccgc ctcggacccg ccgatgccga ccggcacgat cgggaccccg
     6061 gtccgtccgg cgacgaaggc cggaccctcg aacaggtcct cgaccacggg accggtgcgg
     6121 cgctgaccct cggggaacat caccaccggc tcgccgtagc cgagcgcggt gacggccgag
     6181 cgcatcgcct cgcggtcggc ggaaccacgg cgcaccggga tgccgcccat ggcgtcgaag
     6241 aagcggcccg ggaggtcgta cttccacatg gtgtgcttgc ccatgaatcg catccggcgg
     6301 cgggcgacgg cagccacgag cagggagtcc acgttggagc ggtggaccgc ggacagcacg
     6361 aagggcccgt cgggcacctt gtcgaggccg tggacctcca ggcgccagaa cagccggcag
     6421 aagccgacga tgaccgcggt cacggcgcgg tagaagcgca gctgaccctt ggtgggcggg
     6481 ccctgctctt cgaccgggtt gccgcggggg tcggcggcgg ggacgggctc agccatcgat
     6541 ctgctcggcc agcagctcga cgatctcgtc gatggtcagg tcgctggtgt ccacctcgac
     6601 cgcgtcgccg gcgcgggtga gcggcgaggc gtcgcggccc tggtcgagcg catcccggcg
     6661 ggcgatgtcg gcggccacgg tctcgtagtc gaggtcggag acctccttgg accgccgagc
     6721 ggcccgcacc tccggccggg cgtccaggta caccttcagc gcagcgtcgg ggaacacgac
     6781 cgagccgatg tcgcgacctt cgaggacgcc gccgccccgg cgacgggccc actcccgctg
     6841 gcgggacacc atctcggccc ggacaccggg gttggcggcg accgtgctca cggcccgcgt
     6901 cacctcgggg ccacggatct cgatggtggc gtcgacgccg tccaccacga cgccgtccag
     6961 cgtgacggcc aggtcgacct tggcggcgag ggcggcgacc ggttcggcat cggcggggtc
     7021 caggttccgg cgcagcgccg cgaacgtgac ggcccggtac atcgcgccgg tgtcgaggta
     7081 ctccaggccg aggcgctcgg ccaggaggcg ggccacggtc gacttgccgg agccagccgg
     7141 gccgtcgatg gcgatgacgc ggaggccgtc gttggacatg gcggcgacgc taccgctgcc
     7201 gggacggggc gacggagccc gctcgcaggt cgtcgagcag gtcccagtag ccggggaacg
     7261 tcttggccac gcagcccggg tcgcggaccg ccatgccggg gacgacgagg ccgagcaggc
     7321 cgaaggccat cgccatgcgg tggtcccggt aggtgtcgaa cgtggcggag tgcagcgggc
     7381 cggggcggat gaccatgccg tcgtcggtgg cggcggcgtc gatcccgcac cactgcagct
     7441 cggcgacggg cgtcgcgatc cgatcggact ccttgtcccg gatgaagccg acgccccgga
     7501 tcgtcgtggg gctgtcggcg agggcggcga gggcggcgag cgtgggcacg gtgtcggaga
     7561 tgtgggccat gtcgacgtcg acgccgtgca ggtggtcccc tgcggcgacg gtgacggcgt
     7621 cgtcgtgcca cgtcacgtcg gcgcccatct gcccgagcac gtcggcgaag tccacgtcgc
     7681 cctgcagcga cccccggtgg agcccgggca cggtgacccg cccaccggtg acgagggcgg
     7741 cagcccaggc gtaggaggcg gccgaggcgt cgggctcgat ggcgtaggtc gtggggcggt
     7801 acggctcggg ggcgacgtgc caggtctcgc gccccccgcc ctcgtcgtga tcgactcggg
     7861 caccgaaggc ctccatgacc gcgacggtca tgtccacgta gggacgggag atgagctccg
     7921 tggtgaccgt gagctcgagg ccgtccggtg tggccggccc gctgagcagg agccccgaca
     7981 ggaactggct ggacacgtcg cccgggaccg ccagcgagcc cgtgcggagc gggccggcca
     8041 ccgtcacggg caggcggccg gcgccggcct gctcctcgac gcggaccccg gccgaccgga
     8101 gggcgtcgaa ggtcggaccc atggggcggt cccgcagctg cacatcaccg tcgagggtgc
     8161 tcatcccctc gccgagcgcc agcagcggca gcaggaagcg cgccgtggtg ccgctctggc
     8221 gggcgtccac tgcgccggcc cggcgctcgg tggacccgac cacggtcagc tcggcgtggc
     8281 cggtgccggt cacctcgacg cccagggtcc gcagtgcctc caccatcgcc tcggtgtcgt
     8341 cggcgtagag cacccccgtc agcaccgagg tgccgtcggc cagggcggcg cacaccagcg
     8401 cccggttggt gatgctcttg gagccgggga gggtgacggt ggcgtcgatc ggggtgtcga
     8461 cggggacgat caggagcggg tccggcacgg tcagcccagg tgctggatcg acgggcggta
     8521 cccacgggcg agcaggccgc cgcggaagcg ctccacgagc gcgccctcga tgagcacgat
     8581 gagcacgccc tgctggcctt cggaggagtg ggcgatctcg aggtcgacga tgttgacgtc
     8641 gagctcggtg gccagcgtgg tcacgtcggc gagcacgccc gtgcggtcgg gcaccggcac
     8701 gcggacctcg gccagctccg acggtcgggt gtatcgggac gggaggttga cccgcgccgc
     8761 ccgggcccgg gtgaggtcgg cgagcagggc gtcgcggtcc gacgacgcca cctggtcccg
     8821 cgcctcggcg agcgcggcga tgagccggtc gatcccggcg acgatggcca cctggttctc
     8881 ctcgcagatg tcgggccaga tgccggggtg ccccgaggcg atgcgggtca tgtcgcggaa
     8941 gccgccggcc gcgaggcgga gcagcgcggc gtgctcgtcg gcgcgctcgt cggcgatccg
     9001 catcagcgac gcggcggtca ggtggggcac gtgggacacg acggccacca gctcgtcgtg
     9061 gcggtccggc ggcagggcga tgacctcggc gccgaatgtg gagacgatgc cgcggatgcg
     9121 ggcgtaggcg gcatcgtcgg tgtcggccgt gggggtgagc acccacacgg caccctcgaa
     9181 caggtcggcg gtggcgccgt ccacaccctc ctgctccgag cccgccatcg ggtgcccgcc
     9241 gacgaagcgg acgtcgtcca cggccgccac gatcggggcc ttcaccgagc cgacgtcggt
     9301 gaccagcccg gacgtcccgg ccagggcctt gcgggcggcc tcggccaccg tgcgcaccgg
     9361 ggtggcgacg aaggtgatgt cggcgtcggc gtcgagcccg acctggtcga tcacgtcgag
     9421 ctcgcgggcc cggtcggtcc gggcctcgtc gtggtcgacg ccgctgacgt gccagccctc
     9481 ggcgcgcagg gcgagggcga tcgaaccgcc gatcaggccg acgccgacga cgttggcgcg
     9541 tggctgggag gcaccgggca cggtgctcga tcctacgtgg ggggtgccgt ggggaccgca
     9601 gcccgttcca gggcccggac ctcgtcctgg cgcagcggcc gccactcccc cgggccgagc
     9661 ttggtgtcga ccagcgggcc gatccgcgtc cgcaccaggc ggaccacggg atgcccgacg
     9721 gcctcgcaca tccgccggac ctggcggttc cggccctcgt ggatcaccag gcgcaccacc
     9781 cccgggctga ccatcgccgc cgtcgccggc gccgtgatcc cgtcctccag ctcgacgccc
     9841 tctcgcaggg tgcgcagcgc gccgcgcgac ggtgtcccct cgacgtgggc caggtactcc
     9901 ttctccacgc cgtagctcgg gtgggtgagg cggtgcgtga cctcgccgtc gttggtgagc
     9961 agcagcaggc cctcggtctc ggcgtcgagg cgacccaccg ggaacacccg gggctcctcg
    10021 ggcacgagct gcacgaccgt cgggcgaccc tgggggtccg acgccgtgct gaccaccccg
    10081 gccggcttgt tcagcaggta ccagaccgtg tcgggccgga cgccgacgag gacgccgtcg
    10141 acctcgatgg catcgacgtc ggggtcggcc cgctggccga gctcggcgac ctcgccgttc
    10201 acgacgaccc gtccgtcctc gatcatctcc tccacgaccc ggcggctggc gatgccgacg
    10261 cgggcgagga tcttctgcag gcgctcgggc tcgctcactc cgactcgtcg tcggcggcgt
    10321 cggaggcgtg ctcgccgtcg ccatcgccgt ccaccggtgc cgtcgtgtcg gcatcgagcg
    10381 gatccggacg gagcccgtgc tcgagggcct cgtacacgtc cgccccgggc acgaactcgc
    10441 cgagggccgg cagctcgtcg agggagttga ggccgagctt ctcgaggaag gtgggggtcg
    10501 tgccgtagag gacggcctgg cccggtccgt ggtcgcggcc gacctcgtcc acgtagccgc
    10561 gctggacgag cgtgcgcatc acgccgtcga cgttcacgcc tcggatggcg gcgatctggg
    10621 cccgggacat cggctgcttg taggcgacga tggcgagggt ctcgagcgcc gcgttcgaca
    10681 gccggccgga ctggccgtcg agcacgtagc gctcgacgta gggggccagg tccgggtgcg
    10741 tctggtagcg ccaccctccg gcgaccttca ccagctggaa gccccggtcg gtggcgtcgt
    10801 actcggcagc gagctcgacg cacagcgcct cgacgcggtc ggtgctcacc tcgagcagct
    10861 gggccagcag ctcgacggtg atgggctcct cggtgacgag cagcagggct tcggcagctc
    10921 gccgcagctc ccgttcccga tcgtcctcgc ctgcagtggg ttccggctca gtcactctca
    10981 gccatcgtag gcatcgacga aggcgagatc gtcgaggtcg agctcgtcgt cgtcgggacg
    11041 accggtccac accacggtga gcgagccgag gctgttcacc tgctggaggt cgatccagcc
    11101 ctgcttgtac agctcgagca cggccaggaa gcggaccacg acctcgaggc gctcgacgag
    11161 gtcggcggtg agctcggcga aggtggcacg accgatgcgg ggaagctcgt cgaggagctc
    11221 ggcgacggcg tcggtgaccg tgacccggat ctggtggatg tggtcgatgt ggacccgggg
    11281 caccggcttg ggctcgaccg cccggaggaa cgcatcgcgc agctggtcgg gcgtggtggt
    11341 ggcgagcagg tccggcagca ggcccaggaa ctgctcgtcg acaccggcga ggcgcgggta
    11401 ggaccgaccc gcctcgttgg cgatggccgc catggccgcg gccgcatcct tgaacgtctt
    11461 gcactcgacc aggcgggaca gcagcaggtc gcgctcctcc cacagggcga gctcgtcgtc
//
LOCUS       DATISU010000013         1828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_47181, whole genome shotgun sequence.
ACCESSION   DATISU010000013 DATISU010000000
VERSION     DATISU010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47181"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1828
                     /locus_tag="VLR27_00740"
     CDS             <1..>1828
                     /locus_tag="VLR27_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HSP01996.1"
                     /translation="GTFIINGTERVVVSQLVRSPGVIFQPGERFRLRNLSKHQLVTGT
                     IHPYRGEWIEFDVEQKPGKPVTAGTRVARKRRLSMFVLLRALGYDEENAPGFLEAFVR
                     EFDVLEDQREKDRDLAPTQDDALVEIYKRARPGEPPSVDSARNYFRSAFFENRRYDLS
                     RVGRYKLNKKLGPELGRLAEKFPMIELDLPESDQPVLSRCEVLASMTYLLNLVRAEPG
                     YRLDDQDHFANRRIRSVGELIQNQVRIGLSRMERVVRERMTTQDVEAITPQTLINIRP
                     VVAAIKEFFGTSQLSQFMDQVNPLSGLTHRRRLSALGPGGLSRERAGFEVRDVHFSHY
                     GRMCPIETPEGPNIGLIGGLATFAKVNEFGFIQSPYRKVIDGTVTDEIVYLTADEEED
                     YVVAQANALFDENGKFIREKVLVRKSPQAATLGELKLMLERDVFFGATTEIATVPPDE
                     VQMMDVSPKQIISVATALIPFLEHDDANRALMGANMQRQAVPLVRAEAPYIGTGIEGR
                     CAVDAADTIIATDSGTCVEATGDRIVVEYDNEGRKVFNLLKFERSNQDSCINQRPRVV
                     PGQKFAEGDLIADGPSTDMGELALGKNLLVAFMSWEGYNFEDA"
BASE COUNT          310 a          671 c          575 g          272 t
ORIGIN      
        1 cggcaccttc atcatcaacg gcaccgagcg cgtcgtggtg tcccagctcg tccgctcgcc
       61 gggcgtgatc ttccagccgg gtgagcggtt ccgcctgcga aacctgagca agcaccagct
      121 cgtcaccggc accatccacc cctaccgggg tgagtggatc gagttcgacg tcgagcagaa
      181 gccgggcaag ccggtcaccg cgggcacccg cgtggcccgc aagcgccgcc tgtcgatgtt
      241 cgtgctgctg cgcgccctcg gctacgacga ggagaacgcc ccgggcttcc tcgaggcctt
      301 cgtgcgcgag ttcgacgtcc tcgaggacca gagggagaag gaccgcgacc tcgctccgac
      361 ccaggacgac gcgctggtcg agatctacaa gcgggcccgc ccgggtgagc cgccgtcggt
      421 ggactcggcc cggaactact tccggtccgc gttcttcgag aaccgccgct acgacctgtc
      481 ccgggtcggc cgctacaagc tgaacaagaa gctcggtccc gagctcggcc gcctggccga
      541 gaagttcccg atgatcgagc tggacctgcc cgagagcgac cagccggtgc tctcgcgctg
      601 cgaggtcctc gcgtcgatga cgtacctgct caacctggtg cgggccgagc ccggctaccg
      661 cctggacgac caggaccact tcgccaaccg gcgcatccgc tcggtcggcg agctgatcca
      721 gaaccaggtc cgcatcggcc tgtcccgcat ggagcgggtc gtgcgcgagc gcatgaccac
      781 ccaggacgtc gaggcgatca cgccccagac gctgatcaac atccggcccg tggtggcggc
      841 gatcaaggag ttcttcggga cctcccagct gtcgcagttc atggaccagg tgaacccgct
      901 gtcgggtctg acgcaccgcc gtcgcctctc ggcgctcggc cccggtggcc tgtctcgcga
      961 gcgagccggg ttcgaggtgc gagacgtgca cttctcccac tacggccgca tgtgcccgat
     1021 cgagacgccg gagggcccca acatcggcct gatcggcggc ctggccacct tcgccaaggt
     1081 caacgagttc ggcttcatcc agtcgccgta ccgcaaggtc atcgacggca ccgtcaccga
     1141 cgagatcgtc tacctcaccg ccgatgagga agaggactac gtcgtcgccc aggccaacgc
     1201 cctgttcgac gagaacggca agttcatccg cgagaaggtc ctggtgcgca agtcgcccca
     1261 ggccgccacc ctgggtgagc tcaagctcat gctcgagcgc gacgtgttct tcggtgccac
     1321 caccgagatc gccaccgtcc cgccggacga ggtccagatg atggacgtct cgcccaagca
     1381 gatcatctcg gtcgccaccg cgctgatccc cttcctcgag cacgacgacg ccaaccgcgc
     1441 cctgatgggc gccaacatgc agcgccaggc cgtgccgctg gtccgggccg aggcccccta
     1501 catcggcacc ggcatcgagg gccgctgcgc cgtcgacgcc gccgacacca tcatcgccac
     1561 cgactcgggc acctgcgtcg aggccacggg cgaccgcatc gtggtggagt acgacaacga
     1621 gggccgcaag gtcttcaacc tgctcaagtt cgagcgctcc aaccaggaca gctgcatcaa
     1681 ccagcgcccc cgcgtcgtcc cgggccagaa gttcgccgag ggcgacctca tcgccgacgg
     1741 cccgtccacc gacatgggcg agctggccct gggcaagaac ctgctcgtgg ccttcatgtc
     1801 ctgggagggc tacaacttcg aggacgcc
//
LOCUS       DATISU010000014         6863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_419797, whole genome shotgun sequence.
ACCESSION   DATISU010000014 DATISU010000000
VERSION     DATISU010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419797"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..552
                     /locus_tag="VLR27_00745"
     CDS             55..552
                     /locus_tag="VLR27_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01997.1"
                     /translation="MEQHAPGAGAAEGARIVRPRWRARLVRGFVAVVLVGHAVLLVRG
                     YADPHKFFAFQPFNESSTWRADVVRVTADGRRVPIEEPWPGGYDWDELVRWGVLEQPD
                     TMRHAYSGLGTSLDFLDKALDWVADHTPEDDETLYLEATTEAYRNTRGPEVRVFRSDV
                     REEAR"
     gene            549..1667
                     /locus_tag="VLR27_00750"
     CDS             549..1667
                     /locus_tag="VLR27_00750"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HSP01998.1"
                     /translation="MTATATEPVPGAPRLTDRVDDAFDRPASVRALAVLRLALGPVVL
                     VHLWPFLADTLAGTTYEDHFWVRWWGFVPEVPGAVQVALVWTGAVAAVGLTLGWRTRL
                     VAPITCMCVAGNLFLSQHHFRHNRAFLLFLLAAVALADSGRVLSLDARRRRARTGASP
                     TDPGDDLGTVWPLWLLRVLAASVYLASGFSKLIDPDWSGGLVLWDRAVRHQHLVRERV
                     PEPIADAVVELITARWVHTITSPIAVAMELFIGIAIWFGRTRLAAIWVAIFFHASIEL
                     SASVEVFSLAAIVALVIWVTPSTRDRVAVVPDATWVRRLDWLARFEVHERPGAELRVV
                     DRDGTVHTGGTARWLLWSRLPLVFPFVAVAHVLRRARP"
     gene            1664..2815
                     /locus_tag="VLR27_00755"
     CDS             1664..2815
                     /locus_tag="VLR27_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP01999.1"
                     /translation="MNVRRRTMRALVAWAVGASIMRLTLWAPEVCPPIARSEARLAAT
                     QAADWIVVNQADDGEFLYDFHRGRDESSDDYNLVRHAGVTMSLYQLVRGGEVRYLDAA
                     DRGLAWMLDRLQPTGGGGTAFTDVDRAKLGASSLLVVSLAHRRLATSDTQHDQLMQDL
                     GTFLVEQQRADGSMLNFHDIETGEPVPGETSVFATGEALWGLALLHEAFPQVGYDDPA
                     WSTLDYMATSRDLDEDIFPQPWPDQWAAYSLGEMGDWGLADEHIEYARRLIERFAMIV
                     RFDAQRGSTYGSITHGPAPRGGGVGTWLEGLAALRPLTLTDERLADLEDPLEDTLACA
                     AGVLAERQVEPGADVSEQEAGAWFYDDLTRMDDQQHAASGLLLSLPVLP"
     gene            2841..3383
                     /locus_tag="VLR27_00760"
     CDS             2841..3383
                     /locus_tag="VLR27_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HSP02000.1"
                     /translation="MILALLGFLATVNPAAAALALVRDRRTDRPRSVSAGVAVAALVL
                     VLLAAAADPILDLLDINLGTYRLGAGVVIAVAGLRWLVAGAGPACEEPDEDRRLAGFV
                     AFPVLLTPGAVVLAVSVGAEHGWIEAAVGIVVAVVLGGLGLYLRRSLPHLFIGGLVRM
                     LGAAATLLGVVVAVDGIRTL"
     gene            complement(3405..4325)
                     /gene="gluQRS"
                     /locus_tag="VLR27_00765"
     CDS             complement(3405..4325)
                     /gene="gluQRS"
                     /locus_tag="VLR27_00765"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HSP02001.1"
                     /translation="MADGRFAPSPTGPLHVGNLRTALLSWLYARVDGGRFLVRIEDLD
                     PVASREELVEEHLGDLAALGLDHDGPVLRQSDPATRGRHEAAIAQLEADGALYPCFCS
                     RREIREASQAAHGPLPEGAYPGTCRDLGDAERAERAAARPGRPPSLRVRADDARVTIR
                     DRLHGERTGVVDDFVVRRGDGVPAYNLAVVVDDAHQDIAEVVRGDDLLEGTPRQAWLC
                     DRLGLPRPRWVHVPLVVGPDGERLAKRHGSVTRSELAVHGIDATGLLGRMGASLGLAS
                     HGERVDATTLLTRFDPDAVPLERWVFDAPI"
     gene            complement(4380..5054)
                     /locus_tag="VLR27_00770"
     CDS             complement(4380..5054)
                     /locus_tag="VLR27_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HSP02002.1"
                     /translation="MSKTTIRAGAGLAIALSLAAFSWPAANGDDSADTSQAVHAAAAS
                     GPNGIRPVDETAAGDASAALAELGDQIAAEAAAAQQARDLEALAAAEEAANLERLVAA
                     IEAEKLARLAAAIEANEAREAEERRAAAAASAPAGSLEAIIQRHFGSAAPAAIRIAQC
                     ESSMNPNARSATNDHGLFQINIVHRGQFEAVTGAPWSSVYDPELNTIYARHLYAQQGW
                     GPWTCA"
     gene            5364..5455
                     /locus_tag="VLR27_00775"
     tRNA            5364..5455
                     /locus_tag="VLR27_00775"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5400..5402,aa:Ser,seq:gct)
     gene            5552..5636
                     /locus_tag="VLR27_00780"
     tRNA            5552..5636
                     /locus_tag="VLR27_00780"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5586..5588,aa:Ser,seq:tga)
     gene            5657..6034
                     /locus_tag="VLR27_00785"
     CDS             5657..6034
                     /locus_tag="VLR27_00785"
                     /inference="COORDINATES: protein motif:HMM:NF018777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HSP02003.1"
                     /translation="MIKLTFCLRRRPGLSREEFQAYWLETHAPLVQARAEVLGVRRYV
                     QCHTADLPGLHAAFQARNGGAPEPYDGVAELWFDSLEAMTGGAPDPEAAVAAAAELLE
                     DERSFIDLPSSPIWLGYEHEIVG"
     gene            complement(6053..>6863)
                     /locus_tag="VLR27_00790"
     CDS             complement(6053..>6863)
                     /locus_tag="VLR27_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP02004.1"
                     /translation="YLHEIGQVPLLDAARERELAQRIEAGRAARERLDAGEVDVELKR
                     AARLGARAKDEFIRANLRLVVSLAKRYPLPTGMELLDLIQEGNLGLEHAVDKFDWRKG
                     FKFSTYATFWIRQSIGRALDQKSSLIRLPGDRSAQLRAAVRAADANDVELDADLAELQ
                     RLTSPTSLDKPLGDDGDGDFGDLFAGAEQGPEAAAVQQLHQQLVLTLLDELNPRSRHA
                     VRLRLGLDDGRERSFREVGEELGVTAEAARRLVKRAMDELRDVARELELAA"
BASE COUNT          831 a         2516 c         2527 g          989 t
ORIGIN      
        1 cggaccctac cgatgcccgc gagcgctgcc gccgacgcgc cacactcgtc cggcgtggag
       61 cagcacgcgc cgggggcagg cgccgccgag ggagcgcgga tcgtgcgccc ccgctggcgg
      121 gcgaggctgg tgcggggctt cgtcgccgtc gtgctcgtcg gccacgccgt gctcctggtg
      181 cggggctacg ccgacccgca caagttcttc gcgttccagc ccttcaacga gtcctccacg
      241 tggcgggccg acgtcgtgcg ggtcacggcc gacggtcgcc gggtgccgat cgaggagccg
      301 tggcccggtg gctacgactg ggacgagctg gtccgctggg gcgtgctcga gcagcccgac
      361 acgatgcgcc acgcctactc cgggctcggc acgtcgctgg acttcctgga caaggccctc
      421 gactgggtgg ccgaccacac ccccgaggac gacgagaccc tctacctgga ggccacgacc
      481 gaggcctacc ggaacacgcg tggcccggag gtccgagtgt tccgcagcga cgtgcgggag
      541 gaggcacggt gaccgcgacc gccacggaac cggtcccggg cgcgccgagg ctgacggacc
      601 gcgtcgacga cgcgttcgac cgtccggcct cggtgcgcgc cctcgccgtg ctgcgcctgg
      661 cgctcgggcc cgtggtgctg gtgcacctct ggccgttcct cgccgacacc ctcgccggca
      721 cgacctacga ggaccacttc tgggtccgct ggtggggctt cgtccccgag gtgccgggtg
      781 cggtccaggt cgcgctggtg tggacggggg cggtcgccgc cgtggggctc acgctcggct
      841 ggcgcacccg cctggtcgcc ccgatcacct gcatgtgcgt ggccggcaac ctcttcctct
      901 cccagcacca cttccggcac aaccgggcgt tcctgctgtt cctgctggcg gccgtggcgc
      961 tcgccgacag cggtcgggtg ctctcgctcg acgcccgccg gcgacgggcc cgcaccggcg
     1021 cctcccccac cgacccgggt gacgacctcg gcacggtctg gccgctgtgg ctgctgcgcg
     1081 tgctggccgc cagcgtctac ctggcctcgg ggttctcgaa gctgatcgac ccggactggt
     1141 cgggtgggct ggtgctgtgg gaccgggccg tgcgccacca gcacctggtg cgagagcggg
     1201 tgcccgagcc gatcgccgac gccgtcgtcg agctgatcac cgcccgctgg gtgcacacca
     1261 tcacctcgcc gatcgccgtg gccatggagc tgttcatcgg gatcgccatc tggttcgggc
     1321 gcacccgcct cgccgcgatc tgggtcgcca tcttcttcca cgcctccatc gagctgtcgg
     1381 cgtcggtgga ggtgttcagc ctggccgcca tcgtcgccct ggtgatctgg gtgacgccgt
     1441 cgacccggga ccgcgtcgcc gtcgtcccgg acgccacgtg ggtccgccgc ctcgactggc
     1501 tggcgcggtt cgaggtccac gagcgacctg gggccgagct gcgcgtcgtc gaccgggacg
     1561 gcaccgtgca caccggcggc accgctcggt ggctgctgtg gagcaggctt cccctcgtgt
     1621 tcccgttcgt cgccgtcgcc cacgtcctgc gccgggcgcg cccgtgaacg tccgtcgccg
     1681 caccatgcgg gccctggtgg cctgggccgt gggggcctcg atcatgcgcc tgacgctgtg
     1741 ggcaccggag gtctgcccgc cgatcgcccg ctccgaggcc cggctggcgg cgacgcaggc
     1801 cgccgactgg atcgtcgtga accaggccga cgacggcgag ttcctctacg acttccaccg
     1861 gggccgggac gagtccagcg acgactacaa cctggtccgc cacgccggcg tcaccatgag
     1921 cctctaccag ctggtgcggg gcggcgaggt ccggtacctc gacgccgccg accgggggct
     1981 ggcgtggatg ctcgatcggc tgcagcccac cggcggcgga ggcaccgcgt tcaccgacgt
     2041 cgaccgggcc aagctcggcg cgtcgtcgct cctcgtggtg tcgctcgccc accgccggct
     2101 ggccacatcg gacacccagc acgaccagct catgcaggac ctcggcacgt tcctggtcga
     2161 gcagcagcgc gccgacggct cgatgctcaa cttccacgac atcgagaccg gcgaaccggt
     2221 gcccggcgag acatcggtct tcgccaccgg cgaggcgctg tggggcctgg cgctgctcca
     2281 cgaggccttc ccccaggtcg gctacgacga ccccgcctgg agcaccctgg actacatggc
     2341 cacgagccgc gacctggacg aggacatctt cccccagccg tggccggacc agtgggcggc
     2401 gtactcgctc ggcgagatgg gcgactgggg cctggccgac gagcacatcg agtacgcccg
     2461 ccgcctcatc gagcgcttcg ccatgatcgt gcgcttcgac gcccagcggg gctcgaccta
     2521 cggctcgatc acccacggcc ccgcaccccg cggtggcggc gtcggcacct ggctggaggg
     2581 cctcgccgcg ctgcggccgc tcaccctcac cgacgagcgc ctcgccgacc tcgaggaccc
     2641 gctggaggac accctggcct gcgccgccgg ggtgctcgcc gagcgccagg tcgagccggg
     2701 agcggacgtc tccgagcagg aggccggcgc ctggttctac gacgacctca cccgcatgga
     2761 cgaccagcag cacgcggcgt ccggtctgct gctctccctg cccgtcctcc cctgaccccg
     2821 atccccaagg acaccccacc gtgatcctcg ccctcctcgg cttcctcgcc accgtgaacc
     2881 cggccgccgc ggcgctggcc ctcgtccgcg accgtcgcac ggaccggccc cggtcggtga
     2941 gcgccggcgt cgcggtggcc gcgctggtgc tcgtcctgct cgccgcggcg gcggacccca
     3001 tcctcgacct gctcgacatc aacctgggca cctaccggct cggcgccggc gtggtcatcg
     3061 cggtggcggg tctgcgatgg ctggtcgccg gcgcggggcc ggcgtgcgag gagcccgacg
     3121 aggaccgccg gctcgccggg ttcgtcgcct tccccgtgct gctgacgccg ggtgccgtcg
     3181 tgctggccgt ctcggtcggc gccgaacacg ggtggatcga ggcggccgtc ggcatcgtcg
     3241 tcgccgtcgt cctcgggggg ctggggctgt acctgcgccg atcgctcccc catctcttca
     3301 tcggcgggct cgtgcgcatg ctcggggcgg cagcgacgct gctcggcgtc gtcgtcgccg
     3361 tggacggcat ccgcaccctc tgacgtcgcg tcggggtcgc tggctcagat cggggcgtcg
     3421 aacacccaac gctcgagcgg gacggcatcg gggtcgaacc gggtcagcag cgtcgtggcg
     3481 tcgacgcgct cgccgtgcga cgccaggccc agcgacgcac ccatccggcc gagcagcccg
     3541 gttgcgtcga tgccgtggac ggcgagctcg ctgcgggtga ccgacccgtg ccgcttggcc
     3601 aggcgctcac cgtcgggccc gaccaccagc ggcacgtgca cccagcgggg ccggggaaga
     3661 ccgaggcggt cgcacagcca cgcctggcgc ggcgtgccct cgagcaggtc gtcaccgcgg
     3721 acgacctcgg cgatgtcctg gtgggcgtcg tcgaccacga ccgcgaggtt gtaggcgggg
     3781 accccgtcac cccgtcgcac gacgaagtcg tcgaccaccc ccgtgcgctc gccgtgcagg
     3841 cggtcgcgga tggtcacgcg ggcgtcgtcg gcccggaccc gcagtgaggg gggacgtccg
     3901 ggccgggcgg cggcgcgctc ggcccgctcg gcgtcaccga ggtcccggca cgtgcccggg
     3961 tacgcgccct cggggagcgg tccgtgggcg gcctgggatg cctcgcggat ctcccggcgg
     4021 gagcagaagc aggggtagag cgcgccgtcg gcctcgagct gcgcgatggc cgcctcgtgg
     4081 cggccccggg tggccgggtc ggactggcgc agcaccgggc cgtcgtggtc gaggccgagg
     4141 gcggcgaggt cgccgaggtg ctcctcgacc agctcctcgc ggctggcgac ggggtcgagg
     4201 tcctcgatcc gcaccaggaa gcgcccgccg tcgacccgcg cgtacagcca cgacagcagc
     4261 gccgtgcgca ggttgcccac gtgcagcggc ccggtggggc tgggggcgaa gcgaccgtcg
     4321 gccatggcgg ccgagcctac gatcgccccc tcaccccacc ggtcgccagg gcggctcggt
     4381 caggcgcagg tccaggggcc ccagccctgc tgggcgtaga ggtgccgggc gtagatcgtg
     4441 ttcagctcgg ggtcgtacac cgaggaccac ggggcgccgg tcacggcctc gaactggccg
     4501 cggtgcacga tgttgatctg gaacaggccg tggtcgttgg tggcgctgcg ggcgttgggg
     4561 ttcatgctcg actcgcactg ggcgatccgg atggcggcgg gggccgcgga gccgaagtgg
     4621 cgctggatga tggcctcgag ggaaccggcc ggcgcgctgg ctgcggcggc ggcacggcgc
     4681 tcctcggcct cgcgcgcttc gttcgcctcg atcgcggcgg cgaggcgggc gagcttctcg
     4741 gcctcgatgg cagcgaccag gcgctccagg ttggctgcct cctcggcggc ggcgagcgcc
     4801 tcgaggtcac gggcctgctg ggcggcggcc gcctcggcgg cgatctggtc accgagctcg
     4861 gcgagcgccg cgctggcgtc gccggccgcg gtctcgtcga ccgggcggat cccgttgggg
     4921 ccgctcgccg cggcggcgtg cacggcctgg ctggtgtcgg cgctgtcgtc gccgttcgcc
     4981 gccggccagg agaaggcagc gaggctgagc gcgatggcga ggccggcgcc ggcacggatg
     5041 gtggtcttgg acatgcgtgg tcctttccgc cggtgcaggc gcgcgcctgg acgccggcgt
     5101 ggggcatcgg ggggaaggca gccggtcggc ggcgaggcga gtggggatcg cccggcaccc
     5161 gatcgcgctg gcgaccccgg gtccaggggg cgcagcggcg cggtcagcga ccagcagctg
     5221 gttcgatgtt gcagagtctg tgccattcgc aaccgaaacg caacattcct ccctcgtgaa
     5281 cgtggtcaca aggtgcgaac ggcgcgagcg agccgtcgcc ttcggacctg ccggcccgag
     5341 ggggctatcc tccgtcgttc cttggagtgg tgaccgagtc aggccgaagg tgcttccctg
     5401 ctaaggaagt gagggttcac gctctccgag ggttcgaatc cctcccactc cgccaagcgc
     5461 tccgttctgg cctacatcca tgccacctgg tggccaccga tgtaggccag aacgtcgggc
     5521 aggcgggcgc gggctagcct cccggatccc aggaggggtg ccagagcgga cgaatggaac
     5581 ggtcttgaaa accgtcgagc cttcgggttc cgcgggttcg aatcccgccc cctccgccga
     5641 ctgccatcat ccctggatga tcaagctcac gttctgcctg cgacgccgac cgggcctgtc
     5701 ccgggaggag ttccaggcct actggctgga gacccatgcg cccctggtgc aggcgcgggc
     5761 cgaggtcctg ggcgtgcgcc gctacgtgca gtgccacacc gccgacctgc ccgggctcca
     5821 cgccgcgttc caggcgcgca acggcggcgc gcccgagccc tacgacggcg tggccgagct
     5881 gtggttcgac agcctcgagg ccatgaccgg cggcgccccg gatcccgagg ccgcggtcgc
     5941 cgccgcggcc gagctgctcg aggacgagcg cagcttcatc gacctgccca gctcaccgat
     6001 ctggctggga tacgagcacg agatcgtcgg ctgaagcggg atcagcgcct gatcaggcgg
     6061 ccagctccag ctcccgggcc acgtcgcgca gctcgtccat ggctcgcttc acgaggcgac
     6121 gtgcggcctc ggccgtgacg ccgagctcct caccgacctc gcggaagctg cgctcgcggc
     6181 cgtcgtccag gccgaggcgc aggcgcaccg cgtgccggga gcgggggttg agctcgtcga
     6241 ggagcgtgag caccagctgc tggtggagct gctgcacggc tgcggcctcg ggaccctgct
     6301 cggcaccggc gaagaggtcg ccgaagtcgc cgtcgccatc gtcgccgagg ggcttgtcga
     6361 ggctggtcgg tgacgtgagc cgctggagct cggccaggtc ggcatcgagc tcgacgtcgt
     6421 tggcatcggc ggcgcgcacc gcggcacgca gctgggcgga ccggtcgccg ggcaggcgga
     6481 tcaggctgga cttctggtcc agggcccgac cgatggactg gcggatccag aacgtggcgt
     6541 aggtcgagaa cttgaacccc ttgcgccagt cgaacttgtc gaccgcgtgc tcgaggccga
     6601 ggttgccctc ctggatgagg tcgagcagct ccatgccggt cggcagcggg tagcgcttgg
     6661 ccaagctcac caccaggcgc aggttggccc ggatgaactc gtccttggcg cgggcaccca
     6721 accgggcggc gcgcttcagc tcgacgtcca cctcgccggc gtcgagccgc tcgcgggcgg
     6781 ctcggccggc ctcgatccgc tgcgcgagct cacgctcccg ggcggcatcg agcagcggca
     6841 cctggccgat ctcgtgcaag tac
//
LOCUS       DATISU010000015         4880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_140419, whole genome shotgun sequence.
ACCESSION   DATISU010000015 DATISU010000000
VERSION     DATISU010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140419"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..2113
                     /gene="fusA"
                     /locus_tag="VLR27_00795"
     CDS             56..2113
                     /gene="fusA"
                     /locus_tag="VLR27_00795"
                     /inference="COORDINATES: protein motif:HMM:NF009891.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HSP02005.1"
                     /translation="MTERIRNVALVGHAGNGKTMVAEALLLRAGVLGRMGSVEAGTTA
                     SDTDDEERQRGQSLSLSVLPFAWGDHHVNLLDTPGYADFAGEVEAALRGADLLVFVID
                     AVAGVQSEDERLWRVASERGIPRLIFVNKMDRDRADFDRVLAEVRERFSPTGIEPVEL
                     PIGQAADFHGIADLVTEHVWLYDSGRGEERDVLPDDIAAREQVEHEHLVEDVVEQDDQ
                     LLERYLEGDQPTAEELERALHEGVDRAVVFPVLLGSAVGPDGPVAVDRLAEFICHVGP
                     SPAEVAPAVAEAAGDVVEVPCDPDGPPVAFVFKTVTDPYVGQLSMFKVLSGTVAVDDV
                     LIDARTGEKQRLHGLVRVRGAEHEALGRVVAGDVGAVTKLHGLAAGDTLVADGFPVRL
                     PPLELPEPVHAVAITAESQADEDKLAVALQKVLHDDPSLQVVHDEETGQTVLRGAGET
                     QLQVTLERITRRHGVAVEVQPLAVAYRETLAAPAEVEGRHKKQSGGRGQFGVVQVRFE
                     PLERGAGFEFVDAVVGGAVPRNLIPAVEKGIVESMRRGGPHGYRVVDLRATLLDGKHH
                     AVDSDEASFKMAGSLALRAAFEEAGSAVLEPISDVDVTVPTEAQGDVIGDVNARRGQV
                     TGTEAGPGAGEVTVHALVPTAELTTYATDVRSMTHGRGRFRATFDRYEAMSEQLVERL
                     ADR"
     gene            2254..3510
                     /locus_tag="VLR27_00800"
     CDS             2254..3510
                     /locus_tag="VLR27_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02006.1"
                     /translation="MTVLGAVLLIGPPLALATVTPLPTEEAPVVVSVNGTPIDADVGQ
                     MAEGCTVVVEVTGLDPDAPPVSVGVTISAVPPTVPEGTTSVLVQDVRSTAQGQLTAEY
                     DMAPLVEAAGFELKTNGHRLAVAVTLDDTAMGSRELWLACGAPQDGEPNRIVFEVRWL
                     DAAGQPLAVPIDSTMPSGWRDAYAVRASSSRGTATCTVLPGSEQLVCDYDNPGHGSEP
                     GLVVPGGKRHTYVVAQDGLPAGWQLDASTVGTFLGRETCPTEGCGGGDHDDGGGSHDD
                     GSCGGHEEEVTASAATEAEDGATCVHTVVNLQRAPAVPPPIPPPTTTTTTTTTTTTTT
                     TTTVATSTTTSAPPTTTTTVAASVLPTEETATPSPSGPPATVGTVTLPRTGSGFAGLA
                     LVGLALLVSGLVLRVSERTGSTKSPR"
     gene            3521..4048
                     /locus_tag="VLR27_00805"
     CDS             3521..4048
                     /locus_tag="VLR27_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02007.1"
                     /translation="MHTRRRAIAASVAVLALSYGTLGCGDDDTTSADTAETTETQSEL
                     TPATDRDRDRDQDGEGDRDRDRDRDQIHDAIGAISAACQGDQDRLRDMVGDQLRDQVQ
                     DRLRDQSCLLDESDDVEVTEEDIQVDGDTATARFTFQHRHRGELQEDAMTWRFRWTEQ
                     DGWVLDDLDSLFAED"
     gene            complement(4067..>4880)
                     /locus_tag="VLR27_00810"
     CDS             complement(4067..>4880)
                     /locus_tag="VLR27_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP02008.1"
                     /translation="DPAAALRAIEEHGITNMFGVPAMYLFMSQVPGFDQADLSSVRVF
                     VVGGAPCPEPLIEKYLAKGAPFAQGYGLTETAPMASFLLPRDALAKVGSAGQPPLYTD
                     VVCMGEDGEPVAAGQPGEICIKGPNVMAGYWNRPDATAEAIDADGWFHSGDLGYFDDE
                     GYLYVVDRLKDMVISGGENVYPAEVESVLQGHDKVADVAIVGLPDDRWGEAVTAIVVP
                     SGDAPTLEELRDFAGTQLARFKLPTRLQVVDELPRNPAGKILKFELRDRFDG"
BASE COUNT          738 a         1723 c         1787 g          632 t
ORIGIN      
        1 cggtcggggc aggctgcggg acagcgaacc atctcggagg tgcctcgtgg agccagtgac
       61 cgaacgcatc cgcaacgtcg ccctggtggg ccacgccggc aacggcaaga cgatggtggc
      121 cgaggcgctg ctgctgcgcg ccggcgtgct cggccggatg ggctcggtgg aagccggtac
      181 caccgcgtcg gacaccgacg acgaggagcg ccagcgcggg cagtcgctct cgctcagcgt
      241 gctgccgttc gcctggggcg accaccacgt gaacctgctc gacacgcccg gctatgccga
      301 cttcgccggg gaggtggaag cggcgctccg cggcgcggac ctgctcgtgt tcgtcatcga
      361 tgcggtggcg ggcgtccaga gcgaggacga gcggctgtgg cgggtggcgt ccgagcgcgg
      421 catcccgcgc ctgatcttcg tcaacaagat ggaccgtgac cgtgctgact tcgatcgggt
      481 gctcgccgag gtccgggagc ggttcagccc caccggcatc gaacccgtcg agctgcccat
      541 cgggcaggcg gcggacttcc acgggatcgc cgacctggtc accgagcacg tgtggctcta
      601 cgactccggc cgcggtgagg aacgagacgt cctcccggac gacatcgcgg cacgcgagca
      661 ggtcgagcac gagcacctcg tggaggacgt ggtcgagcag gacgaccagc tcctcgagcg
      721 ctacctcgag ggggaccagc ccacggccga ggagctggag cgcgcgctgc acgagggcgt
      781 cgaccgggcc gtggtgttcc ccgtgctgct cggctcggcc gtgggaccgg acggaccggt
      841 cgccgtggac cgcctggccg agttcatctg ccatgtcggc ccctcgcccg ccgaggtcgc
      901 cccggcggtg gcggaggcgg ccggcgacgt ggtggaggtg ccctgcgacc ccgacggacc
      961 tccggtggcg ttcgtgttca agaccgtcac cgacccctac gtcggccagc tctccatgtt
     1021 caaggtgctg tccggcacgg tggctgtcga cgacgtcctc atcgatgcgc gcaccggcga
     1081 gaagcagcgg ttgcacggcc tcgtccgggt gcgtggggcc gagcacgagg cgctggggcg
     1141 cgtggtggcc ggggacgtcg gggcggtgac gaagctgcac ggtctggccg ccggcgacac
     1201 cctcgtcgcc gacggcttcc cggtgcggct cccgccgctc gagctgccgg agccggtcca
     1261 cgccgtggcc atcacggccg agagccaggc cgacgaggac aagctggccg tggcgctgca
     1321 gaaggtgctc cacgacgacc cgtcgctgca ggtcgtgcac gacgaggaga ccgggcagac
     1381 ggtgctccgc ggcgccggcg agacccagct gcaggtgacg ctcgagcgga tcacccggcg
     1441 ccacggcgtc gcagtcgagg tgcagccgct cgccgtcgcc taccgggaga cgctggcggc
     1501 gccggcggag gtcgagggcc ggcacaagaa gcagtcgggc gggcggggtc agttcggtgt
     1561 ggtccaggtg cgcttcgagc cgctcgagcg cggcgccggc ttcgagttcg tcgacgccgt
     1621 cgtcggtggc gccgtccccc gcaacctcat ccctgcggtg gagaagggca tcgtcgagtc
     1681 gatgcggcga ggcggaccgc acggctaccg cgtcgtcgac ctccgagcca cgctgctcga
     1741 cggcaagcac catgctgtgg actccgacga ggcgagcttc aagatggcgg gttcgctcgc
     1801 cctgcgagcc gcgttcgagg aggcaggttc ggcggtgctc gagccgatct ccgacgtcga
     1861 cgtgacggtg cccaccgaag cccagggcga cgtgatcggc gacgtcaacg cccggcgcgg
     1921 ccaggtcacc ggcaccgaag ccggccccgg ggcgggtgag gtgacggtcc acgccctcgt
     1981 gcccaccgcc gagctgacga cctacgccac cgacgtgcgg tcgatgaccc acgggcgggg
     2041 gcggttccgg gccacgttcg accgctacga ggccatgagc gagcagctgg tggagcgact
     2101 cgccgaccgg tagccgcgac acggcaccgt gccccggcgg tggtgaccgc agactctggc
     2161 ggagggcagc agcccagcgc gaacgctcga tccatggcgg cgcacacatc tcgaaggcgg
     2221 accctccggc cgaaggcaca gggaacgaca gcgttgacgg tgctcggcgc cgtgctgctg
     2281 atcggccctc ctctggcgct ggcgacggtc acgccgctcc cgaccgagga ggctccggtc
     2341 gtcgtcagcg tcaacggcac gcccatcgac gccgacgtgg gacagatggc cgagggttgc
     2401 accgtcgtgg tcgaggtcac cggcctcgat cccgacgcgc ccccggtgtc cgtcggcgtc
     2461 acgatcagcg cggtgcctcc gaccgtgccc gagggcacca ccagcgtcct cgtccaggac
     2521 gtccgctcca ccgcccaggg gcagctcacc gccgagtacg acatggcacc gctcgtggaa
     2581 gcggcggggt tcgagctcaa gaccaacggt caccggctcg ccgtggccgt cacgctcgat
     2641 gacaccgcga tgggatcgcg ggagctctgg ctcgcctgcg gcgcgccgca ggacggcgag
     2701 ccgaaccgca tcgtgttcga ggtgcggtgg ctcgacgccg ccgggcaacc cctggcggtg
     2761 ccgatcgaca gcaccatgcc gtccggctgg cgagacgcct acgcagtccg cgcatcgagc
     2821 agccgcggga cggccacgtg cacggtcctg cccgggtcgg agcagctggt gtgcgactac
     2881 gacaaccccg gccacggctc ggaacccggc ctcgtggtac ctggcggcaa gcgccacacc
     2941 tacgtggtgg cacaggacgg cctgcccgcg ggctggcagc tcgatgcatc gacggtcggc
     3001 accttcctgg gacgggagac ctgccccacc gaggggtgcg gcggtggcga ccacgacgac
     3061 ggtggcggga gccacgacga cggcagctgc ggcgggcacg aggaggaggt gaccgcgagc
     3121 gcggccacgg aggccgagga cggcgcgacc tgcgtccaca cggtcgtcaa cctgcagcgt
     3181 gcgccggcgg tgccgccgcc gatcccgccc ccgacgacca ccaccaccac cacgacgacg
     3241 acgaccacca cgacgaccac gacggtggcg accagcacca cgacgagcgc gcctccgacg
     3301 accacgacga cggttgccgc ttcggtcctc ccgaccgagg agacggcgac gccgtccccc
     3361 tcggggccgc cggcgacggt cgggaccgtg acgttgcccc gaacggggtc cgggttcgcc
     3421 gggctggcgc tggtcggact ggcgctgctc gtcagcggcc tggtcctacg ggtctcggag
     3481 cggaccggaa gcacgaagtc acctagatga aggagatccc atgcacacca gacgacgggc
     3541 gatcgctgcg agcgtcgccg tcctcgcgtt gagctacggc acgctcggct gcggtgacga
     3601 tgacacgacg tccgcagaca ccgccgagac gaccgagacc cagtcggagc tgacccccgc
     3661 gaccgaccgc gaccgtgacc gtgaccagga cggcgaaggg gaccgtgacc gtgaccgcga
     3721 tcgtgaccag atccacgacg cgatcggggc catctcggcg gcgtgccagg gagaccagga
     3781 ccgcctgcgc gacatggtcg gcgaccaatt gcgcgaccag gtgcaggatc ggctgcggga
     3841 ccagagctgc ctgctcgacg agtccgacga cgtcgaggtc accgaggagg acatccaggt
     3901 cgacggtgac acggcgaccg cccgcttcac gttccagcac cgccaccgtg gcgagctgca
     3961 ggaggacgcg atgacctggc ggttccgctg gaccgaacag gacggctggg tcctcgacga
     4021 cctcgactcg ctgttcgccg aggactgacc gcggctgagg gctgcgtcag ccgtcgaagc
     4081 ggtctcgcag ctcgaacttg aggatcttgc cggccgggtt ccgggggagc tcgtccacca
     4141 cctggagccg ggtcggcagc ttgaaccggg ccagctgggt ccccgcgaag tcgcgcagct
     4201 cctccagggt cggggcgtcg ccgctcggca ccacgatggc ggtgacggcc tcgccccacc
     4261 ggtcgtcggg caggccgacg atggccacgt cggcgacctt gtcgtggccc tgcagcaccg
     4321 actcgacctc ggccgggtac acgttctccc cgccggagat gaccatgtcc ttgaggcggt
     4381 ccacgacgta caggtagccc tcgtcgtcga agtagccgag gtcgccggag tggaaccagc
     4441 cgtcggcgtc gatggcctcg gcggtggcgt cgggccggtt ccagtacccg gccatgacgt
     4501 tgggaccctt gatgcagatc tcgccgggct gacccgcagc gaccggctcg ccgtcctcac
     4561 ccatgcagac gacgtcggtg tacagcggcg gctggcccgc cgagccgacc ttggccagtg
     4621 cgtcgcgggg cagcaggaag ctggccatgg gcgcggtctc ggtgaggccg tagccctggg
     4681 cgaacggggc acccttggcc aggtacttct cgatgagcgg ctccgggcac ggcgccccgc
     4741 cgaccacgaa cacccgcacg ctcgacaggt cggcctggtc gaacccgggc acctggctca
     4801 tgaacaggta catggccggc accccgaaca tgttggtgat gccgtgctcc tcgatggccc
     4861 gcagcgcggc agccgggtcg
//
LOCUS       DATISU010000016        16086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_699313, whole genome shotgun sequence.
ACCESSION   DATISU010000016 DATISU010000000
VERSION     DATISU010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 16086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16086
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699313"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VLR27_00815"
     CDS             complement(<1..842)
                     /locus_tag="VLR27_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP02009.1"
                     /translation="MAIGAYFHVPDPSPVVADEHAIATLQEAIAEVVPDRECIVWRDR
                     RLTWRQVTERSRRLANGLLDAGVGGDVVHDADAPGWVSPHDHVALYLTNGNEYLEAML
                     GAWKAGAAAINVNYRYTAEELAFVLADAGAVAVVYHARFAPVLAVALPHVPSLRLLLV
                     VDDGTGTTPVEGSIGYEALLAGASGARPDLTWSADDRYVVYTGGTTGNPKGVLWRQGD
                     FLHTALGVRGSQDELVERARRPDRPRALPAPPFMHGAAHWTALSCLLAGGTVVIQHDT
                     EHLDA"
     gene            835..1659
                     /locus_tag="VLR27_00820"
     CDS             835..1659
                     /locus_tag="VLR27_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSP02010.1"
                     /translation="MATPSADLTTVLAEDVRPGVRLLTMNRPDRLNAMDHQLVSELHD
                     RFDEVAADPDLRCVILTGAGRGFCAGLDLKGSAAVPGTEELGRPQRGLATQQHIATLI
                     PHLRSLKVPVIAAVNGPAAGGGFALALGADVRVCATSARFNVAFVRIGLSGCDIGVSW
                     MLPRLIGAGRAFELLLTGRIFDAQEADDLGLVTKVVEDGAVVDAALEIADNVVANSPF
                     GVWMTKEVMWSQLEIPSLQAGIDLENRTQILSSFTEDSTEAIQAFLGKRAPEFKNR"
     gene            complement(1656..2132)
                     /locus_tag="VLR27_00825"
     CDS             complement(1656..2132)
                     /locus_tag="VLR27_00825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSP02011.1"
                     /translation="MAKPYDMQIDLDTVYEVTIDTDKGTIVGDLDPQLAPKTVNEFVS
                     KARDGFYDGLTFHRVVPDFVIQGGDPEGTGRGGPGYKFEDEPVKGEYTEGAFAMANAG
                     PNTNGSQFFITIDDCTRKLDKLYNLFGYVTQGMDVAKSIAVGDTMKSVKIEEKSRS"
     gene            complement(2152..2763)
                     /locus_tag="VLR27_00830"
     CDS             complement(2152..2763)
                     /locus_tag="VLR27_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP02012.1"
                     /translation="MVSEQRRSRTRLDPAVRRRQIAEAAARVFSEHDPSEVSFELIAD
                     EAGVSRSLVYSYFGDRGSLFAAAYNHELERLDTEIDGALESLGSDRERLARAVSAYLG
                     FAWRHRTSWQLIASASSSRHPAVREAIQARTDRIAASITDTPEARLLVRGVIGMLEAA
                     AIHTLENEDVDPDGLAELLTQIIWDGISSLDGDGPRAPEAPPE"
     gene            complement(2792..3829)
                     /locus_tag="VLR27_00835"
     CDS             complement(2792..3829)
                     /locus_tag="VLR27_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HSP02013.1"
                     /translation="MAGTGTAHLRPEADVLLRVEDLRVEFPSGRGRVVHAVSDISFDI
                     APGETLGLVGESGCGKSTTGKAIMRLNPITSGRVVFDGRDLAKLSGSELRKVRPELQM
                     IFQDPVSSINPRRRIFDVVAEPLKVWDRMDGAEELIDRVLDDVGLTPEIAKPKRRHEF
                     SGGQCQRICIARALVLDPKLIICDEPVSALDVSVQAQILNVLEDMKERYGLTMMFIAH
                     DLAVVKNISDRVAVMYLGKMCEVASPDDLFARPAHPYTATLLSAIPIPDPDVVPSAMD
                     LDGELPSPLDPPSGCRFRTRCPRAAERCTTEEPLVREVGPDHYVACHFPLIGEEAPPE
                     ASTPVEVGSQQ"
     gene            complement(3829..4932)
                     /locus_tag="VLR27_00840"
     CDS             complement(3829..4932)
                     /locus_tag="VLR27_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP02014.1"
                     /translation="MARRRIVAEPSKDQTEPLLVVDDLHTTFRTPSGRVKAVNGVSFE
                     LQQGRTLGIVGESGSGKSVLSRTIMGLIPSNNVEVTGSAKYRGNELVGAKSNVLRSLW
                     GSELSMIFQDPMTSLNPVMRIGKQITEVLRHHLDMSNDEADEMAVSLLRSVKIPEPEK
                     RVRQYPHELSGGMRQRVVIAVALACGPHLLFADEPTTALDVTVQAQILDLLDEQQRER
                     FMSMVLVTHDLGVVAGRAHEIAVMYAGRIVEMAPTRTLFQNMRMPYTQALFESIPKLD
                     LPSHTRLTVIPGRPPDLVNPPTGCSFAPRCERAQDRCLEEQPPLMEAETPDHRYACFY
                     PVGTAEGDEALAENRRRGYQAAGVPVVIQEKAS"
     gene            complement(4953..5861)
                     /locus_tag="VLR27_00845"
     CDS             complement(4953..5861)
                     /locus_tag="VLR27_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP02015.1"
                     /translation="MAGPTATDEDSGVTLSKTKRLGPIGWLAIMWLALVALMAVVPQL
                     FPVPGLTERFPDALRERDFGPASGHPLGFDTSGRDLLAKLVYGTRASMIVSVAAITFG
                     LVVGGTLGLIAGYFRGKIDTVITNAFNVGLAIPQLVLAITLAAVFASDANAGYGQRVA
                     VVTFAIGFSSIPILGRITRANTLTWANREFVMAAKTVGAKTPRIIIREVLPNVAPAMF
                     SIALLGIAVAIVVEGGLALIGVGIPADITSPSWGNLIATGRSQMLLGEAAQVMTASLM
                     VFFTVLSLNYLGDVVRARFDVRDSAL"
     gene            complement(5905..6855)
                     /locus_tag="VLR27_00850"
     CDS             complement(5905..6855)
                     /locus_tag="VLR27_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP02016.1"
                     /translation="MNNALRKLGQLALVLFLVTLFTALLVSLLPGDPAEVIIPFGDES
                     DREALREELNLDDPLPVRYVAWLGDFVTGDLGNYYRQSITEPVADRVTQAAPVSLLLM
                     LYAQVLALVIAIPLGVLTAQRAGSLFDKFTNTTAFGILAIPNFVLALVLSYVVGVELG
                     WLPPQGYVRFSNDVGEHFKSMILPAVSLAAGQVAVYMRLLRTDMVATLQEDFILMAKA
                     KGLSNKRVLWRHALRPSSLTLLTVAGLNVGTLIGGALIIEIIFTLPGLGVLIFQAISE
                     RQYVAIQSLVALIAVAFVLVNVAVDMLYAALDPRIRSARG"
     gene            complement(6898..8610)
                     /locus_tag="VLR27_00855"
     CDS             complement(6898..8610)
                     /locus_tag="VLR27_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSP02017.1"
                     /translation="MTSRNPTRRWIRLLALLLGLSMFAAACGDDDDGGEGATPDENGT
                     ETETEDSVDSTPEDAEGDPVMGGDLTYALEAETNGGFCLPEAQLAIAGMQVATTVYDT
                     LTKPDADGEIQPYLAESVEPNEDFTQWTITVPEGRTFHDGSPLDAQVVANNLDAYRGA
                     YEGRNPALFVFVFQNIESVEVIDPMTVQVNMATPWTAFDWFLWGSNRVGILAQAQLDD
                     AETCGTNLIGTGPFQLEEWTVNEEFVATKFADYWQTDEDGNQLPYLDSITYVPIPEVA
                     SRGNALESGQVDVLHTSDTEQIAQRLQPLADSDEIKLLQTDAYGEVNYLMLNTSQAPF
                     DNKNARLALAHALDRQLLIDVRGGGLGQVADGPFAEDVTGYVDDPGFPQYDVEMARQF
                     AQAYEDETGSALSFSYTFVSTESGQLTAQEIQTQLADAGITINLDPAGDQATTINNAL
                     AGDFQALGWRNHPGADPDTQYNWWATGSPVNFGRIDDPELQSLLDEGRQAPPGEEREG
                     IYQDLTRRFAEEVYNVWTSTTVWGIAADPSVQNLLGYGPQGGSGAFPGLGTGHDVTGI
                     WISE"
     gene            8983..10416
                     /locus_tag="VLR27_00860"
     CDS             8983..10416
                     /locus_tag="VLR27_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSP02018.1"
                     /translation="MSDAVDVVVIGAGPNGLAAAIEVARAGCSVTVLEARDTPGGGAR
                     TSELTGPGFHHDVCSAIHPLATGSPFFRSVPLAEHGCELVHPDIPVVHPLDGGRAGVF
                     HRSVAETVEAAGVDGPAWEALFGPLVEHWEDIETHLLGPVVRPPRHPISMARFGLQAI
                     RRAESVSAGFRTDETRALFAGVAAHAFLPLDRVLTASFGLMLGGLAHARGWPMVRGGT
                     QQLTDALVSYLEDLGGTVVTDCHVTSLDELPPHRAVIADISPHQLVPMAGDRLDARGR
                     RRLERFRHGPGACKVDFALSGPVPWANEEARRAGTVHLAGTYEELALSETITAAGGHP
                     DRPFVLAAQQSLFDDTRAPEGQHTLWTYAHVPNGSTLDVSERMTDQIERFAPGFRDLI
                     IDKHVITAAEYERYNPTFVGGDIGGGSHGGTQLVLRPWPAIDPYATPVEGVFLCSAST
                     PPGGGVHGMCGSHAARSTLRWLGKQTA"
     gene            10471..11292
                     /locus_tag="VLR27_00865"
     CDS             10471..11292
                     /locus_tag="VLR27_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HSP02019.1"
                     /translation="MSDRVQIDVADGVAHVRLNRPDKMNALDGAMFAAIAEAGETVKA
                     DPSIRAVVLSGEGRAFCSGLDFGSFQAMADGPKADDGDTASMGAIGETGDRITHLGQQ
                     VAHVWQEVPVPVIAAIHGVALGGGIQIALGADIRIIAPDARMSVLEVKWGLVPDMTGT
                     WMLRRLVGLDVAKELTFTGRMVEGEEAVRIGLATRVAADPLAEAKALAAEIAGKSPDA
                     VRAAKRLLNQSGVVPIEQQFADERREIGALIGSPNQVEAVMSGFEKRDPSFTDPS"
     gene            complement(11316..12908)
                     /locus_tag="VLR27_00870"
     CDS             complement(11316..12908)
                     /locus_tag="VLR27_00870"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HSP02020.1"
                     /translation="MPAPLVPFHTLRARKGMVCSVDHLASSAGIGVLRAGGNAVDAAI
                     ATSAALAVTTQHMCGMGGDLFALVHHADGQEPTCLNASGRAGSGADAGQLRNEGHHRM
                     PTDEDIRAVTVPGCVDGWAALHERHGRLPLTDVLEPARVLAEEGFPASPLLSFMVPII
                     EQVDGADDYTRQPVHEGTVIRRPGVARTLAAIASDGRAAFYEGEFGEGLRRVGGGLFS
                     ADDLARSQADWIAPLGVDLWGHRAWTVPPNSQGYLSLAAARIAELAGFGPDRAHRPGS
                     EEWAHLLVEAARFAGHDRIAVLHDEADGDALLADDRLAPMADRFDPDRRAAPPAPSGA
                     GDTIYLCTADGEGQAVSLIQSNARGWGVHLAVPEVGIFLHNRGQGFSLEPGHPAEYRP
                     GRRPPHTLAPALVQRGDGSLRAVLGTMGGDTQPQVVLQLLARLLVADQDPARCIGAPR
                     WRLGDGGFDTWEGDGPGLVGVERGAPDAWVEGLRRRGHDVSVQDPWNSGYGHAHLIER
                     TTDGTLAGAADPRALTGATATW"
     gene            12940..14343
                     /locus_tag="VLR27_00875"
     CDS             12940..14343
                     /locus_tag="VLR27_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSP02021.1"
                     /translation="MEAHRLRSMLRALRSVVGDEHVLVDPDVVASHAADWTGGFVGTT
                     PAVVRPGSTAEVAEVVAACRRDGVAVVAQGGNTGLVGGSVPLAGEVVLSTSRLVGITD
                     VDTDAGQLTAGAGETLAAVQTAALGAGWRYAVDLAARDSATIGGTVATNAGGLRVLRH
                     GMTRHQLLGVEAVLGTGDVVSRLGGLVKDNTGYDLAGLLCGSEGTLGIVTRARLALVP
                     AAGSSVTSLIGLATVADAVALVGHLRRAVPTLEAAELVLADGARLVAAQTGVAPVLDP
                     VPPVLVVVEAVGPPDPTDALAVALESAPGVLDVAVATDAARAAALWHVREAHTEAIAR
                     IGVPRKYDVTVPAPRLARFVEEVPGLVRDVDPDAQVWIFGHLGDGNLHVNVTSTPSRA
                     APSRTGSAAVAPDPVAEAVLGRVVADGGSVSAEHGIGTAKRAWLLRDRSAGDVAAMRS
                     IKAALDPDGILNPGVLL"
     gene            complement(14377..14715)
                     /locus_tag="VLR27_00880"
     CDS             complement(14377..14715)
                     /locus_tag="VLR27_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HSP02022.1"
                     /translation="MGPGHAERLAEERARAVRQVASLQGSFDDIVAASEQVATDDEHD
                     PEGHTIAWERQQVAALLEAARRHLAELDLAIRRVEGGDYGRCEVCGRSIPDERLEAIP
                     TASTCVSCAD"
     gene            complement(14696..>16086)
                     /locus_tag="VLR27_00885"
     CDS             complement(14696..>16086)
                     /locus_tag="VLR27_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP02023.1"
                     /translation="LTYELDVGRGDVVAWLCGNTHELLEAYYGVLLAGAVLLPLNIRL
                     SGAELRGVLEQSRATTLFRHPDQPDPGSPVRQVVVDAGFEEALARQPSGPVEPDVRDE
                     DAPAEIFFTSGSTGMPKGAVLTHRNLYLHAVHNALTNAITGDDVILHLVPLFHVNGWG
                     TPHYLTGLGGTHVMLPRFDAGEVLRLIEAHGVTRLFAVPAMVRMLLDHPDIATRDLSS
                     VRTASIGGAPVSPAMLTEIETAFGCTAICGYGMTEASPTLTRSLDKPGEAPSAERRAT
                     TGLPIVGADVRVLGDGDVEVPWDGETVGEVCARSNHVMAGYLDAPEATAEVIRDGWLR
                     TGDLAIVRPDGYLQIVDRKKDLIISGGENIATPEVEHALCEHPAVREAAVVGRPDARW
                     GEVPVAYVSLHTGASAAPEELVAFVRERLAHFKAPKSVVVLAELPKVGTGKIDKSGLR
                     MQTGGSDGAGPR"
BASE COUNT         2182 a         5513 c         6028 g         2363 t
ORIGIN      
        1 gcgtcgaggt gctcggtgtc gtgctggatc acgaccgtgc cgccggcgag caggcaggag
       61 agggcggtcc agtgggccgc accgtgcatg aacgggggag caggcagcgc tcgtggccgg
      121 tcggggcgac gagcgcgctc gaccagctcg tcctgcgagc cgcggacgcc gagcgccgtg
      181 tggaggaagt cgccctggcg ccacaggacg cccttggggt tcccggtggt gccgcccgtg
      241 tagacgacgt agcggtcgtc ggccgaccac gtgaggtcgg gccgggcacc cgacgcgccg
      301 gcgaggagcg cctcgtaccc gatcgaaccc tcgacggggg tggtgccggt gccgtcatcg
      361 acgacgagga gcaggcgcag cgacggcacg tgggggagcg cgacggccag caccggggcg
      421 aagcgggcgt ggtagacgac ggcgaccgcg ccggcgtcgg ccagcacgaa cgcgagctcc
      481 tcggcggtgt agcggtagtt gacgttgatg gccgcggcgc cggccttcca cgcgccgagc
      541 atcgcctcga ggtactcgtt gccgttcgtg aggtagagcg cgacgtggtc gtgcgggctg
      601 acccagcccg gggcgtccgc atcgtggacg acgtcgccgc cgaccccggc gtcgaggagg
      661 ccgttggcca ggcgtcgtga ccgctccgtg acctgtcgcc acgtgagcct gcggtcccgc
      721 cacacgatgc actcgcggtc ggggacgacc tcggcgatgg cctcctgcag ggtggcgatc
      781 gcgtgctcgt cggcgacgac ggggctgggg tcgggcacat ggaagtatgc accgatggcc
      841 accccctccg ccgacctgac caccgtgctc gccgaggacg tccgacccgg cgtccgcctg
      901 ctgacgatga accggcccga ccgtctcaac gccatggatc accagctggt gtccgagctg
      961 cacgaccgct tcgacgaggt cgccgccgac cccgatctgc gctgcgtcat cctcaccggt
     1021 gccggccgtg gcttctgcgc cggcctcgac ctgaagggca gcgccgccgt gcccggtacc
     1081 gaggagctcg gtcgccccca gcgcgggctg gccacccagc agcacatcgc caccctgatc
     1141 ccgcacctgc gctccctgaa ggtgccggtg atcgcggcgg tgaacggacc ggcggccggc
     1201 ggcggcttcg ccctggcgct cggcgccgac gtgcgcgtgt gcgccacctc ggcccgcttc
     1261 aacgtggcct tcgtgcgcat cggcctgtcg ggctgcgaca tcggggtcag ctggatgctg
     1321 ccccgcctga tcggtgccgg tcgggccttc gagctgctgc tcaccggccg gatcttcgac
     1381 gcgcaggagg ccgacgacct cggcctggtc accaaggtcg tggaggacgg cgccgtggtg
     1441 gacgccgccc tcgagatcgc cgacaacgtg gtggccaaca gccccttcgg ggtctggatg
     1501 accaaggagg tgatgtggag ccagctggag atcccgagcc tgcaggccgg catcgacctg
     1561 gagaaccgga cccagatcct gtcgtcgttc accgaggact cgaccgaggc gatccaggcc
     1621 ttcctcggga agcgagcccc cgagttcaag aaccgctagg agcggctctt ctcctcgatc
     1681 ttcaccgact tcatggtgtc gcccacggcg atggacttgg cgacgtccat gccctgggtg
     1741 acgtagccga acaggttgta gagcttgtcc agcttgcggg tgcagtcgtc gatggtgatg
     1801 aagaactggc tgccgttggt gttggggccg gcgttggcca tggcgaatgc gccctcggtg
     1861 tactcgccct tcaccggctc gtcctcgaac ttgtagcccg ggccgccgcg ccccgtgccc
     1921 tcggggtcgc cgccctggat cacgaagtcg ggcacgacgc ggtggaaggt gaggccgtcg
     1981 tagaacccgt cgcgggcctt gctgacgaac tcgttcacgg tcttgggggc gagctgcggg
     2041 tcgaggtcgc cgacgatcgt gcccttgtcg gtgtcgatgg tgacctcgta gacggtgtcg
     2101 aggtcgatct gcatgtcgta gggcttggcc atgggcttcg accctagcgg cctactccgg
     2161 cggtgcctcc ggggcccttg ggccgtcgcc gtcgagggag gagatgccgt cccagatgat
     2221 ctgggtgagc agctcggcga ggccgtcggg gtcgacgtct tcgttctcca gggtgtggat
     2281 cgccgccgcc tcgagcatcc cgatgacgcc ccggacgagc agccgggcct cgggggtgtc
     2341 ggtgatcgac gccgcgatgc ggtcggtgcg ggcctggatc gcctcgcgca cggcggggtg
     2401 ccgcgacgag ctggctgacg cgatgagctg ccaggacgtg cggtgccgcc acgcgaagcc
     2461 gaggtacgcg gacacggccc gggccaggcg ctcccgatcc gatccgagcg actcgagggc
     2521 gccgtcgatc tcggtgtcga gccgctcgag ctcgtggttg taggcggcgg cgaacaggct
     2581 gccgcggtcg ccgaagtagg agtacaccag gctgcgcgac acgccggcct cgtcggcgat
     2641 cagctcgaag gagacctcgc tcggatcgtg ctcggagaac acccgcgccg cagcctcggc
     2701 gatctgacgt cgccggaccg cggggtcgag ccgcgtcctc gatctccgtt gttcgctcac
     2761 cacggcctcg gtcccccctc cggccggtcg atcactgctg ggatccgacc tccaccgggg
     2821 tggatgcctc gggcggtgcc tcctcgccga tgagcgggaa gtggcaggcc acgtagtggt
     2881 cggggccgac ctcgcggacg aggggctcct cggtggtgca ccgctccgcg gcccgcgggc
     2941 agcgggtgcg gaaccgacag ccggacgggg ggtcgagcgg ggagggcagc tccccgtcga
     3001 ggtccatggc gctcgggacg acgtcggggt cggggatcgg gatcgccgac agcagcgtcg
     3061 cggtgtacgg gtgggcgggg cgggcgaaca ggtcgtcggg agaggcgacc tcgcacatct
     3121 tgccgaggta catcaccgcg acccggtccg agatgttctt cacgaccgcg aggtcgtggg
     3181 cgatgaacat catcgtcagg ccgtagcgct ccttcatgtc ctcgaggacg ttcaggatct
     3241 gggcctggac cgacacatcg agggcggaca ccggctcgtc gcagatgatc agcttcgggt
     3301 cgagcaccag ggcccgggcg atgcagatgc gctggcactg gccgccggag aactcgtggc
     3361 ggcgcttggg cttggcgatc tccggtgtga ggccgacatc gtcgagcacg cggtcgatga
     3421 gctcctcggc gccatccatg cggtcccaga ccttcagcgg ctcggcgacc acgtcgaaga
     3481 tgcgacgccg cgggttgatc gaggacaccg ggtcctggaa gatcatctgc agctcgggac
     3541 ggaccttgcg cagctcggac ccgctcagct tggccaggtc gcgaccgtcg aagaccacgc
     3601 ggcccgaggt gatcgggttg aggcgcatga tcgccttgcc ggtggtcgac ttgccgcagc
     3661 ccgactcgcc cacgagcccg agggtctcgc cgggtgcgat gtcgaagctg atgtcggaca
     3721 cggcgtgcac gacgcggccg cgaccggacg ggaactcgac ccgcaggtcc tcgacccgca
     3781 ggaggacgtc ggcctccggt cggaggtgtg cggtaccggt accagccatc agctcgcctt
     3841 ctcctggatc acgacgggga cgccggcagc ctggtacccg cggcgacggt tctcggccag
     3901 cgcctcgtct ccctcggcgg tgccgacggg gtagaagcag gcgtagcggt ggtcgggagt
     3961 ctcggcctcc atgaggggcg gctgctcctc gaggcagcgg tcctgggccc gctcgcaccg
     4021 gggggcgaac gagcaccccg tgggcgggtt caccaggtcc ggcggacgac ccgggatgac
     4081 cgtgagccgg gtgtggctgg gcaggtcgag cttggggatc gactcgaaca gcgcctgcgt
     4141 gtacggcatg cgcatgttct ggaacagcgt ccgggtcggg gccatctcga cgatgcgtcc
     4201 ggcgtacatg accgcgatct cgtgggcccg gccggcgacg acgccgaggt cgtgggtgac
     4261 cagcaccatc gacatgaagc gctcgcgctg ctgctcgtcg agcaggtcga ggatctgggc
     4321 ctgcaccgtc acgtcgaggg cggtggtggg ctcgtcggcg aagagcaggt gggggccgca
     4381 ggccagggcg acggcgatga cgacgcgctg gcgcatgccg ccggacagct cgtgggggta
     4441 ctggcgcacc cgcttctcgg gctcggggat cttcaccgag cggagcaggg acacggccat
     4501 ctcgtcggcc tcgtcgttgc tcatgtcgag gtggtggcgc aggacctcgg tgatctgctt
     4561 gccgatccgc atgaccgggt tcagcgacgt catcggatcc tggaagatca tcgacagctc
     4621 cgagccccac agggatcgca gcacgttgga ctttgcgccg acgagctcgt tgccgcggta
     4681 cttggccgac ccggtcacct cgacgttgtt cgaggggatc aggcccatga tggtgcgcga
     4741 cagcacggac ttgccggagc cggactcacc cacgatcccc agggtccggc cctgctgcag
     4801 ctcgaaggac accccgttga cggccttcac ccgtccggac ggcgtccgga acgtggtgtg
     4861 caggtcgtcc acgacgagca gcggctcggt ctggtccttc gatggttcgg cgacgatgcg
     4921 acgtcttgcc acgttccccc ctgtgctttc ggtcagagtg cgctgtcgcg tacgtcgaag
     4981 cgtgcccgca cgacgtcgcc caggtagttg aggctgagga cggtgaagaa caccatcaac
     5041 gatgcggtca tcacctgtgc tgcttccccg agcagcatct gggaccgccc ggtggcgatg
     5101 aggttgcccc acgacggcga ggtgatgtcg gccgggatgc cgacgccgat cagggcgagg
     5161 ccgccctcca cgacgatggc gacggcgatg ccgagcaggg cgatggagaa catggccggg
     5221 gccacgttcg gcaggacctc gcggatgatg atgcgcgggg tcttggcgcc gacggtcttg
     5281 gccgccatca cgaactcgcg gttggcccag gtgagcgtgt tggcccgggt gatgcgaccg
     5341 aggatcggga tggaggagaa gccgatggcg aaggtcacca cggccacccg ctggccgtag
     5401 ccggcgttgg cgtcgctggc gaagacggcg gcgagggtga tggccagcac cagctggggg
     5461 atggcgagac cgacgttgaa ggcgttggtg atgaccgtgt cgatcttgcc gcggaagtag
     5521 ccggcgatga ggccgagggt gccgcccacg acgagcccga aggtgatggc ggccacgctc
     5581 acgatcatgg aggcccgggt gccgtagacg agcttggcga gcaggtcgcg accggaggtg
     5641 tcgaagccga gcgggtgacc cgaggcgggt ccgaagtcgc gctcgcgcag ggcgtcgggg
     5701 aaccgctcgg tgaggccggg taccgggaac agctggggca ccacggccat cagggccacc
     5761 aacgccagcc acatgatggc cagccacccg atggggccga ggcgcttggt cttggacagc
     5821 gtgacgccgc tgtcctcgtc ggtggcggtg ggtccggcca cggggccttc gcctgcggac
     5881 gggtcgatct ggacggaggt gggctcaacc tcgggcactg cggatcctcg ggtcgagggc
     5941 ggcgtagagc atgtcgacgg ccacgttcac gagcacgaac gcgacggcga tgagggcgac
     6001 cagcgactgg atcgccacgt actggcgctc gctgatggcc tggaagatca ggactccgag
     6061 accgggcagg gtgaagatga tctcgatgat gagggcgccg ccgatcaggg tgccgacgtt
     6121 gaggccggcc accgtgagca gcgtcaggct ggacgggcgc agggcatggc gccacagcac
     6181 ccgcttgttg gacaggccct tggccttggc catgaggatg aagtcctcct gcagggtcgc
     6241 caccatgtcg gtgcgcagca ggcgcatgta gacggcgacc tgtccggccg ccaggctgac
     6301 cgccggcagg atcatcgact tgaagtgctc gcccacgtcg ttgctgaagc ggacgtagcc
     6361 ctgcggtggc agccaaccca gctccacgcc gaccacgtac gacagcacga gcgcgagcac
     6421 gaagttgggg atggccagga ttccgaaggc cgtggtgttg gtgaacttgt cgaagaggct
     6481 gccggctcgc tgcgccgtga gcacgccgag cgggatggcg atgaccaggg ccaggacctg
     6541 tgcgtagagc atgagcagga gcgagacggg cgccgcctgg gtgacccggt cggcgacggg
     6601 ctcggtgatg ctctggcggt agtagttccc caggtcgccg gtgacgaagt cgccgagcca
     6661 ggcgacgtag cggaccggga gggggtcgtc gaggttcagc tcctcgcgga gcgcctcccg
     6721 gtccgactcg tcaccgaagg ggatgatgac ctcggccgga tcgcccggca ggaggctgac
     6781 gagcagggcg gtgaacaggg tcaccaggaa cagcacgagc gcgagctgtc ccagcttccg
     6841 tagtgcgttg ttcacgtgtc ctgcttcctg ctcgtcagcc tctgggtcac cgctggatca
     6901 ctcgctgatc cagatgccgg tcacgtcatg tccggtgccg agacccggga aggctcccga
     6961 gccgccctgc gggccgtagc cgagcaggtt ctgcacgctc gggtcggcgg cgatgcccca
     7021 tacggtcgtg gaggtccaca cgttgtagac ctcctcggcg aagcgccggg tgaggtcctg
     7081 gtagatgccc tcgcgctcct cgccgggcgg ggcctggcgg ccttcgtcga gcagcgactg
     7141 gagctccggg tcgtcgatgc ggccgaagtt cacgggcgag ccggtggccc accagttgta
     7201 ctgcgtgtcc ggatcggcgc cggggtggtt gcgccagccc agggcctgga agtcgccggc
     7261 gagggcgttg ttgatggtgg tggcctggtc gccggccggg tccaggttga tcgtgatgcc
     7321 ggcatcggcc aactgggtct ggatctcctg ggcggtcagc tggcccgact ccgtggagac
     7381 gaaggtgtag gagaacgaga gggcagagcc cgtctcgtcc tcgtaggcct gggcgaactg
     7441 gcgggccatc tccacgtcgt actgcgggaa gcccgggtcg tcgacgtagc cggtcacgtc
     7501 ctcggcgaac gggccgtccg cgacctggcc gaggccaccg ccacgcacgt cgatcagcag
     7561 ctgccggtcg agggcgtggg cgagcgcgag gcgggcgttc ttgttgtcga acggcgcctg
     7621 cgacgtgttc agcatgaggt agttgacctc gccgtacgcg tcggtctgga gcagcttgat
     7681 ctcgtcagag tcggcgagtg gctgcaggcg ctgggcgatc tgctcggtgt cggaggtgtg
     7741 gagcacgtcc acctggcccg actcgagggc gttgccccgg ctggcgacct cggggatcgg
     7801 cacgtaggtg atcgagtcga ggtagggcag ctggttgccg tcctcgtcgg tctgccagta
     7861 gtcggcgaac ttggtggcga cgaactcctc gttcaccgtc cattcctcga gctggaacgg
     7921 gccggtgccg atgaggttgg tgccgcaggt ctcggcgtcg tcgagctggg cctgggccag
     7981 gatgccgacg cggttggagc cccagaggaa ccagtcgaac gcggtccacg gggtggccat
     8041 gttgacctgg acggtcatcg ggtcgatgac ctcgacggac tcgatgttct ggaacacgaa
     8101 cacgaacaga gccgggttcc ggccctcgta ggcgccgcgg taggcgtcga ggttgttggc
     8161 cacgacctgc gcgtcgaggg ggctgccgtc atggaacgtg cggccctcgg ggacggtgat
     8221 ggtccactgg gtgaagtcct cgttgggctc caccgactcg gccaggtacg gctggatctc
     8281 gccgtcggca tcgggcttgg tcagcgtgtc gtagaccgtc gtggcgacct gcatgccggc
     8341 gatggccagc tgggcctcgg ggaggcagaa gccgccgttg gtctcggcct cgagggcgta
     8401 ggtgaggtcg ccgcccatga ccgggtcgcc ttcggcgtcc tcgggcgtgg agtcgacgct
     8461 gtcctcggtc tcggtctccg tgccgttctc gtccggcgtg gcaccttcgc cgccgtcgtc
     8521 gtcatccccg cacgcagcgg cgaacatcga caaccccagc agcagagcga gcaaccggat
     8581 ccaccggcgc gtcgggtttc ggctcgtcac atttccccct tggtctcggc gtcgggaggg
     8641 tcccgcacgc ccgttgtgca ccgtcctcgg gtggcgacgg tcacgtctgc gggacgctaa
     8701 gtcgcttgtt ggcacagtgt caatagcgct cggcaagtta cttgaccgga cggtcaggtt
     8761 tcccagcccg ggtgacacta ggcacaggga cggctgtcga acgtgcaccc ccgccgccgt
     8821 catggagtcg tcacggaggc cgtcgccgcc ggaaacgggc aggcggggcg aatcggtcag
     8881 cgtgtcggct cctgcgtgag gacggcggca cgctgcgtgc ggaacggtgc gatccggggc
     8941 gtggtcggtg cgtccggggc ccgcacgggc caggctggac ccatgagcga cgcagtggac
     9001 gtggtggtga tcggcgcggg ccccaacggg ttggcggcgg cgatcgaggt ggcccgcgcc
     9061 gggtgctcgg tgacggtgct cgaggcgcgc gacacgccgg gcggtggggc ccgcaccagc
     9121 gagctcaccg gccccgggtt ccaccacgac gtctgctcgg cgatccaccc cctcgccacc
     9181 gggtccccgt tcttccgcag cgtcccgctg gccgagcacg gctgcgagct ggtccacccc
     9241 gacatcccgg tggtgcaccc gctggacggc ggtcgggccg gggtcttcca ccgctcggtc
     9301 gcggagacgg tcgaggcagc aggcgtcgat ggcccggcgt gggaggcgct gttcggcccg
     9361 ctggtcgagc actgggagga catcgagacc cacctgctcg gtccggtcgt gcgcccgccc
     9421 cgccacccga tctcgatggc ccgcttcggc ctccaggcga tccgccgggc cgagtcggtg
     9481 tcggccggct tccgcaccga cgagacgcgg gcgctcttcg ccggggtggc ggcgcacgcc
     9541 ttcctgcccc tggaccgggt cctcaccgct tcgttcgggc tcatgctcgg cgggctggcg
     9601 cacgcccgcg ggtggccgat ggtgcggggc ggcacccagc agctcaccga tgcgctggtg
     9661 tcctacctcg aggacctcgg cggcaccgtc gtcacggact gccacgtcac ctccctcgac
     9721 gagctgccgc cgcaccgcgc cgtcatcgcc gacatcagcc cccaccagct cgtgccgatg
     9781 gcgggcgacc gcctggacgc ccggggccgc cgtcggttgg agcgcttccg ccacggtccg
     9841 ggcgcgtgca aggtcgactt cgccctctcc ggccccgtcc cgtgggcgaa cgaggaggcc
     9901 cgccgggccg gcaccgtcca cctcgccggc acctacgagg agctcgcgct gtcggagacg
     9961 atcaccgccg ccggtggcca ccccgaccgt cctttcgtgc tggccgcgca gcagagcctg
    10021 ttcgacgaca cccgggcgcc ggagggccag cacacgctct ggacctacgc ccacgtgccg
    10081 aacggctcga ccctcgacgt ctccgagcgc atgaccgacc agatcgagcg cttcgccccc
    10141 ggcttccgcg acctgatcat cgacaagcac gtgatcaccg cggccgagta cgagcgctac
    10201 aaccccacct tcgtcggcgg ggacatcggc ggcggctccc acggcggcac gcagctggtg
    10261 ctgcgtccct ggcccgccat cgacccctac gccaccccgg tcgagggcgt gttcctctgc
    10321 tcggcctcga ccccgcccgg cggtggcgtg cacggcatgt gcggctccca cgcggcccgc
    10381 tcgacgctgc gctggctcgg caagcagacg gcgtaggcca gactggcaac gctcgggacc
    10441 agaacccgcc ccactgacag gagtgcagac atgagcgatc gcgtccagat cgacgtcgcc
    10501 gacggcgtgg cccacgtccg gctgaaccgc cccgacaaga tgaacgccct ggacggggcg
    10561 atgttcgccg ccatcgccga ggccggcgag acggtgaagg ccgatccctc catccgggcc
    10621 gtcgtgctct ccggcgaggg ccgggcgttc tgctcgggcc tcgacttcgg ctcgttccag
    10681 gccatggccg acgggcccaa ggccgacgac ggcgacaccg cgtcgatggg cgccatcggc
    10741 gagaccggcg atcgcatcac gcacctcggc cagcaggtcg cccacgtctg gcaggaggtg
    10801 ccggtgccgg tcatcgccgc catccacggg gtggccctgg gcggcggcat ccagatcgcc
    10861 ctcggcgccg acatccgcat catcgccccc gacgcccgaa tgagcgtgct cgaggtcaag
    10921 tggggtctgg tgcccgacat gaccggcacc tggatgctgc ggcgcctggt cggcctcgac
    10981 gtcgccaagg agctcacgtt caccggccgc atggtcgagg gtgaggaggc cgtgcgcatc
    11041 ggcctggcca cgcgggtcgc cgccgatccg ctcgccgagg ccaaggccct ggccgccgag
    11101 atcgccggca agagccccga tgccgtgcga gccgccaagc ggctgctgaa ccagtccggc
    11161 gtggtgccga tcgagcagca gttcgccgac gagcggcgcg agatcggtgc cctgatcggc
    11221 agccccaacc aggtcgaggc cgtgatgtcg ggcttcgaga agcgggaccc gtccttcacc
    11281 gaccccagct gatccacggg ctgatcagcg gctgatcacc aggttgccgt cgcgcccgtg
    11341 agggcgcggg ggtcggccgc acccgcgagc gtgccgtcgg tggtgcgctc gatcaggtgg
    11401 gcgtggccgt agccgctgtt ccacgggtcc tggacggaga cgtcgtggcc ccgccggcgc
    11461 aggccctcga cccaggcgtc gggcgcgccg cgctcgacgc cgaccagccc gggcccgtcg
    11521 ccctcccagg tgtcgaagcc gccgtccccg aggcgccagc gaggcgctcc gatgcagcgc
    11581 gccgggtcct ggtcggcgac gagcaggcgg gccagcagct ggagcacgac ctggggctgg
    11641 gtgtcgcccc ccatggtccc gagcacggcg cgcaggcttc cgtcgcctcg ctgcaccagc
    11701 gccggggcga gggtgtgcgg gggccggcga ccgggccggt actcggccgg gtggccgggc
    11761 tcgagggaga agccctgccc ccggttgtgc aggaagatcc cgacctcggg cacggcgagg
    11821 tgcacccccc aaccccgggc gttggactgg atgagggaga cggcctgccc ctcgccgtcg
    11881 gcggtgcaca ggtagatggt gtcgccggct ccggacggcg ccggcggggc ggcgcggcgg
    11941 tccgggtcga agcggtcggc catgggggcg aggcggtcat cagcgaggag ggcatcgcca
    12001 tcggcctcgt cgtgcaggac ggcgatgcgg tcgtggccgg cgaagcgggc cgcttcgacc
    12061 agcaggtggg cccactcctc gctgcccggg cggtgagccc ggtcggggcc gaagccggcg
    12121 agctcggcga tgcgggcggc ggcgaggctg aggtagccct ggctgttcgg cggcacggtc
    12181 caggcccggt gcccccagag gtcgacgccg agcggggcga tccaatcggc ctggctgcgg
    12241 gcgaggtcgt cggcgctgaa caggcccccg cccacccggc gcagcccctc cccgaactcg
    12301 ccctcgtaga acgcggcgcg cccgtccgag gcgatcgcgg ccagcgtgcg ggccaccccg
    12361 ggccggcgga tgacggtgcc ctcgtgcacc ggctggcggg tgtagtcgtc ggcgccgtcc
    12421 acctgctcga tgatcgggac catgaacgac agcaggggtg aggcggggaa gccctcctcg
    12481 gccaggaccc gagccggttc gagcacgtcg gtgaggggga ggcggccgtg gcgctcgtgc
    12541 agcgccgccc acccgtcgac gcagcccggc acggtgacgg cgcggatgtc ctcgtcggtg
    12601 ggcatgcggt ggtgaccctc gttccggagc tgcccggcgt ccgcgccgga gccggcccgc
    12661 ccgctggcgt tcaggcacgt cggctcctga ccgtcggcgt ggtggacgag cgcgaacagg
    12721 tcaccgccca tcccgcacat gtgctgggtg gtgacggcga gcgccgcgct ggtggcgatg
    12781 gcggcgtcga cggcgttgcc gccggcccgg agcacgccga tgccggcgct cgacgcgagg
    12841 tggtcgacgc tgcagaccat gcccttccgg gcgcggaggg tgtggaacgg cacgaggggg
    12901 gcgggcacgg cgccggaccg taccgggcac acttggcggg tggaggccca ccgactgcgc
    12961 tcgatgctgc gggcgctccg gtccgtcgtc ggggacgagc acgtgctcgt cgatcccgac
    13021 gtcgtggcgt cccacgccgc cgactggacc ggcggcttcg tcgggacgac cccggcggtg
    13081 gtgcgccccg gctcgaccgc cgaggtggcc gaggtcgtcg ccgcctgccg ccgcgacggg
    13141 gtcgccgtcg tcgcccaggg cggcaacacc ggcctggtcg gtggcagcgt gcccctcgcc
    13201 ggcgaggtgg tgctctccac gtcgcgcctg gtcggcatca ccgacgtcga caccgacgcc
    13261 ggtcagctca ctgccggtgc gggcgagacg ctggccgcgg tgcagacggc ggcgctcggc
    13321 gccggctggc gctacgccgt ggacctcgcc gcccgtgact ccgccaccat cggcgggacc
    13381 gtcgccacca acgccggcgg cctccgggtg ctgcgccacg ggatgaccag gcaccagctg
    13441 ctcggcgtcg aggcggtgct cggcacgggc gacgtggtgt cccggctggg cgggctggtc
    13501 aaagacaaca ccggctacga cctcgccggc ctgctctgcg gcagcgaggg caccctcggg
    13561 atcgtgacca gagcacgcct ggccctggtg cccgccgccg gttcgtcggt gaccagcctg
    13621 atcgggctgg ccaccgtcgc cgacgccgtg gcgctggtcg gccacctgcg ccgggcggtg
    13681 ccgacactcg aggccgccga gctggtcctg gccgacggcg ctcggctggt ggcggcccag
    13741 accggcgtcg ccccggtgct cgatcccgta cccccggtgc tggtggtcgt cgaggcggtc
    13801 ggcccgcccg acccgaccga cgcactcgcg gtcgccctcg agtccgcgcc cggggtgctg
    13861 gacgttgcag tcgccaccga cgcggcccgg gccgcggccc tgtggcacgt ccgggaggcc
    13921 cacaccgagg ccatcgcccg catcggcgtg ccccgcaagt acgacgtgac cgtgccggca
    13981 ccccgcctgg cccgcttcgt cgaggaggtc cccggcctgg tccgcgacgt cgaccccgac
    14041 gcccaggtat ggatcttcgg tcacctcggc gacggcaacc tccacgtcaa cgtgacctcc
    14101 accccctccc gggccgcccc ctcccgaact ggcagtgctg cggtcgcgcc ggaccccgtc
    14161 gccgaggccg tcctcggacg cgtggtcgcc gacggcgggt cggtgagcgc cgagcacggg
    14221 atcggcacgg ccaagcgggc gtggttgctg cgcgaccgct ccgccgggga cgtcgccgcc
    14281 atgcggtcga tcaaggccgc cctcgacccc gacgggatcc tgaaccccgg cgtgctgctg
    14341 tgaccagccc ggtccgaccg gcccgcctcg aggtgactag tccgcgcagc tgacgcaggt
    14401 gctggcggtg gggatcgcct cgaggcgctc gtcggggatg gaccggccgc agacctcgca
    14461 gcgcccgtag tcgccgccct ccacccgccg gatggcgagg tcgagctcgg ccaggtggcg
    14521 gcgggcagcc tcgaggaggg cggccacctg ctggcgctcc cacgcgatgg tgtggccctc
    14581 cgggtcgtgc tcgtcgtcgg tggccacctg ctcggacgcg gcgacgatgt cgtcgaagct
    14641 cccctggagc gacgcgacct gccggacggc cctggctcgc tcctcggcga gccgctcagc
    14701 gtggcccggc cccatctgac ccgcccgtct gcatccggag cccgctcttg tcgatcttgc
    14761 cggtgccgac cttcggaagc tcagccagga cgaccaccga cttcggcgcc ttgaagtggg
    14821 cgagccgctc gcgcacgaac gcgaccagct cctcgggcgc ggcggacgcg ccggtgtgca
    14881 gcgacacgta ggcgacgggc acctcgcccc agcgggcgtc gggccggccc acgacggcgg
    14941 cctcgcggac ggcagggtgc tcgcacaggg cgtgctcgac ctcgggggtg gcgatgttct
    15001 cgccgccgct gatgatcagg tccttcttgc ggtcgacgat ctgcaggtag ccgtcgggtc
    15061 gcacgatggc caggtcgccg gtgcgcagcc agccgtcgcg gatcacctcg gcggtggcct
    15121 cgggcgcgtc caggtagccg gccatgacgt ggttcgaccg ggcgcagacc tcgccgacgg
    15181 tctcgccgtc ccacgggacc tcgacgtcgc cgtcgccgag cacgcgcacg tcggcgccga
    15241 cgatgggcag cccggtggtg gcgcgccgct cggcggaggg cgcctcgccg ggcttgtcca
    15301 gcgaccgggt cagcgtcggg ctggcctcgg tcatgccgta gccgcagatg gccgtgcagc
    15361 cgaacgcggt ctcgatctcg gtgagcatcg ccgggctgac gggcgccccc ccgatggacg
    15421 ccgtgcgcac cgacgacagg tcacgggtgg cgatgtcggg gtggtcgagc agcatccgca
    15481 ccatggcggg caccgcgaac aggcgggtga cgccgtgggc ctcgatcagg cgcagcacct
    15541 cgccggcgtc gaagcggggg agcatcacgt gcgtgccgcc caggccggtg aggtagtgcg
    15601 gcgtgcccca gccgttgacg tggaagagcg ggacgaggtg gaggatgacg tcgtcgccgg
    15661 tgatggcgtt ggtcagcgcg ttgtggacgg cgtgcaggta caggttgcgg tgggtgagca
    15721 cggcgccctt gggcatgccg gtgctgccgc tggtgaagaa gatctcggcc ggggcgtcct
    15781 cgtcgcgcac gtcgggttcg accggcccgg atggctgacg agccagcgcc tcctcgaagc
    15841 cggcgtcgac cacgacctgg cggaccggcg accctgggtc gggttggtcg gggtggcgga
    15901 acagcgtcgt cgcacgtgac tgctcgagca cgcctcgcag ctccgccccc gacaggcgga
    15961 tgttcagggg gagcagcacg gcaccggcga ggagcacgcc gtagtacgcc tcgagcagct
    16021 cgtgggtgtt cccgcacagc caggcgacga cgtcgccacg ccccacgtcc agctcgtagg
    16081 tgaggt
//
LOCUS       DATISU010000017         7798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_839086, whole genome shotgun sequence.
ACCESSION   DATISU010000017 DATISU010000000
VERSION     DATISU010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839086"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VLR27_00890"
     CDS             <1..245
                     /locus_tag="VLR27_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSP02024.1"
                     /translation="EEEAIAIANDSMYGLSGGVQGGDVERAKAVARQIRTGQIEVNQG
                     AFNPNAPFGGYKQSGYGREYGTHGFEEFLETKSMQL"
     gene            complement(322..1401)
                     /locus_tag="VLR27_00895"
     CDS             complement(322..1401)
                     /locus_tag="VLR27_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HSP02025.1"
                     /translation="MTQDPGQPEPDAETGADPAGIRVGPVTEWLAEHVGTVAPLRFER
                     LAGGHSNLTYRIDDAEGRRVVLRRPPEGELLPTAHDMGREHRLIEALGPTDVPVPEAL
                     ATCTDAAVIGAPFYVMAHVDGVVLHTAADAEAAFDESERARIGRHFVDVLARLHAVDP
                     DAVGLGDLSRKEDYIARQLKTWWRQYEASQTGEDPLLGQVNEWLHGNIPEQGPARVVH
                     GDYRLGNTISSPDARIAAVLDWEIATLGDPLADLGYVLATWPRPGDEHVATTSATSMA
                     AGFPERAELLQAYEAATGADLAGIDYYVAWANWKTACIVQGVYRRYLDGQLSTEGVDV
                     EGFRHTVEAATELAATAIRRVDPTF"
     gene            1484..2443
                     /locus_tag="VLR27_00900"
     CDS             1484..2443
                     /locus_tag="VLR27_00900"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HSP02026.1"
                     /translation="MRAPRVQPGSDGLEKPMDGERAANVSVRVGGQAVLEGVMMRAGQ
                     RACVAVRRPDGEIDTRMLDVPGWAHRAESIPFVRGLAGLGESLVVGVEALRWSEGAAG
                     FRPSDRTPAPKWLLVSIALVAVVVLIVVLPTGLAGLVAGGTAWFAAVEAAARITVLGT
                     YVALVGRRRDVRRVFEYHGAEHLVVSGYEDRKVLRPDQLRGGSIHHPRCGTSFLLVVA
                     AIAAVVHPFLPHGSLVLQLAGRILAVPLIVAVSYEVLTALGRVAALRPGSRLDRILVW
                     PQRFTTRRPDDAQVEVAIAALQGALGPVSVVPTGPGVLAASRS"
     gene            2645..3643
                     /locus_tag="VLR27_00905"
     CDS             2645..3643
                     /locus_tag="VLR27_00905"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HSP02027.1"
                     /translation="MPASKPPVTVAVTGAAGQIGYSLLFRIASGALLGPDQPVHLKLL
                     EITPAMGALEGVVMELDDCAFPLLAGYTTSDDPNVAFDGAQVALLVGSMPRKAGMERA
                     DLLEANGGIFTVQGKALNDNAADDVKVLVVGNPANTNALIAMHSAPDIPRERFTAMTR
                     LDHNRAIAQLAKKAGATVNDIRKMTIWGNHSATQYPDVFHAEVDGKNGFEAAGSDQSW
                     LESDFIPTVQKRGAAIIDARGSSSAASAANAAIDHVHDWVLGSPEGDWVSMAVPSDGS
                     YGVPEGIISSFPCTTEDGRWSIVQGLEIDDFSRARIDASAGELVEERDTVASQGLI"
     gene            complement(3701..4537)
                     /locus_tag="VLR27_00910"
     CDS             complement(3701..4537)
                     /locus_tag="VLR27_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HSP02028.1"
                     /translation="MPELPEVQAHAERLTEEFAGAVLERFQPIAFTALKTFSPDPAVT
                     HGEPLESVGRRGKLLLLRFPSATHVVHLMQGGRLRPDPKQAAKPKGGQARWRFDDGRA
                     LLLSEAGTERKAGVWVVDGDPEDHEPLDHLGPDATEVDADAMLALLHEENTRVHGFLR
                     DQRRLAGLGRRLANEVLHRAKLSPFAMTAKLGPHDAAALVAAITGAVDEGLAYERTRD
                     DMSSSKDRPGAVHHRTGEACPVCGDTIREVAYTRYTVHYCPTCQTGGKVLADNTTSKF
                     LK"
     gene            complement(4539..4919)
                     /locus_tag="VLR27_00915"
     CDS             complement(4539..4919)
                     /locus_tag="VLR27_00915"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HSP02029.1"
                     /translation="MQFLSDEWLAAMDGAARARPVPDPDPLADATVTIEQVVRDGPRW
                     RLVVDRGRCTVTAGGDGEPDVRLTSDRETAVDIATGRRPALDAFIAGDLVLGGDVRAL
                     LDNRAALEVLGDLFAEVKAETVFS"
     gene            4951..5850
                     /locus_tag="VLR27_00920"
     CDS             4951..5850
                     /locus_tag="VLR27_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02030.1"
                     /translation="MAPRPADEHPHEPVDGDWSETWSFDFASADGELGGWAALTRFTD
                     HAWYQAALTGPRRQLLMVLDHHVPFRSNPMEVRTTGLWADHICETPFDHWTLGLETFA
                     LGVDDPRELHGRQHGDNVALGFDLEWETDGPVAEHPQPAGVHGYELACRVYGEVLVGR
                     DVIDFEGVGSRSHHWGPLDWVVDDGWHLSGVVEGPTRWSVDHRDGTTTALLETDGTTR
                     RVAASGEVEIDEDGLPVAARVEVDGTELRVEVLAPTPVPIELRDRQIRRPRALCRLRS
                     SGGWTGVGWLELRAGAGGSLGAG"
     gene            complement(5858..6781)
                     /locus_tag="VLR27_00925"
     CDS             complement(5858..6781)
                     /locus_tag="VLR27_00925"
                     /inference="COORDINATES: protein motif:HMM:NF015951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HSP02031.1"
                     /translation="MRFRSQLPMQVFRGFCMGAADVVPGVSGGTVALVFGIYATLVAQ
                     IRQGARALGQLVRGRPGGFVEELRKVEWRFLLPLLLGIGVALVSLASVISHFLEEEPI
                     RTAAVFFGLVVGSLFSTVHLVHRWDARNVTTAAVVAVATFLVLGLGSGQIQDPPLAFV
                     FVGGSIAICAMILPGISGSFLLLMLGLYQFVLDSVDERDLVVIAVFGTGAVLGLALFS
                     SLLSWALERHEQPILAGLIGLMFGSLRVLWPWPNGTGSEESTNGAELALPAGDVWWPI
                     ALALLAGAAIVIVARLAGASEAVEHAEVPAR"
     gene            complement(6825..7625)
                     /locus_tag="VLR27_00930"
     CDS             complement(6825..7625)
                     /locus_tag="VLR27_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HSP02032.1"
                     /translation="MPRHPFRYLKHAAAVAAAGTSAVGGVGLLAYVQARMAGIGGAAS
                     YPVHWLDGDVGPADPSAHRVVWLGDSLAAGLGAIVPDVTLPRLVASHSERGTRLHVFA
                     TPGANSNDVVDVQLPALLQLRHGLGQIGQRIDAVGVTVGANDIAAFTSRRRFRRNIAT
                     IAAAADAPLIVVSIPGLADAIRLPHPLRALASVRGRWLDRVLQDAARSSDRIHYANVW
                     ERPPWIERRHRAHFLSGDRYHPSGAGYAVWADRVAHAFELALRPVTPA"
BASE COUNT         1091 a         2705 c         2965 g         1037 t
ORIGIN      
        1 ccgaggagga ggcgatcgcc atcgccaacg actcgatgta cgggctctcc ggtggcgtcc
       61 agggcggcga cgtcgagcgg gccaaggccg tcgctcgcca gatccgcacc ggccagatcg
      121 aggtcaacca gggcgcgttc aacccgaacg ctcccttcgg tggctacaag cagtccggct
      181 acggccgcga gtacggcacg cacggcttcg aggagttcct cgagaccaag agcatgcagc
      241 tctagccagc acggctgacg aacgcatcgg tggcgggccc tcgggcccgc caccggcgcg
      301 tccgggctcc catcgtcggg ttcagaacgt ggggtcgacc cggcggatgg cggtggcggc
      361 cagctcggtg gcggcctcga ccgtgtgccg gaagccctcg acgtcgacgc cctcggtgga
      421 gagctggccg tccaggtagc ggcggtacac gccttgcacg atgcacgccg tcttccagtt
      481 ggcccaggcg acgtagtagt cgatgccggc caggtcggcg ccggtggccg cctcgtaggc
      541 ctggagcagc tcggcgcgct cggggaagcc ggccgccatc gaggtggccg aggtggtcgc
      601 gacgtgctcg tcgcccggcc gtggccaggt ggccagcacg tagccgaggt cggcgagggg
      661 gtcgccgagg gtggcgatct cccagtcgag caccgccgcg atgcgggcgt cggggctgct
      721 gatcgtgttg ccgagccggt agtcgccgtg cacgacccgg gccgggccct gctcggggat
      781 gttcccgtgc agccactcgt tcacctgtcc gagcagcggg tcctcgcccg tctgcgatgc
      841 ctcgtactgg cgccaccagg tcttgagctg gcgggcgatg tagtcctcct tgcgggacag
      901 gtcgccgagg cccacggcat cggggtccac ggcgtgcagc cgggccagga cgtcgacgaa
      961 gtggcgaccg atgcgcgccc gctcggactc gtcgaaggcg gcctcggcgt cagccgcggt
     1021 gtgcagcacc acgccgtcga cgtgggccat cacgtagaag ggggcaccga tcacggcggc
     1081 gtcggtgcag gtggcgaggg cctccggcac ggggacgtcg gtggggccga gcgcctcgat
     1141 caggcggtgc tcgcggccca tgtcgtgcgc ggtcgggagc agctcgccct ccgggggtcg
     1201 ccgcagcacg acccgtcgcc cctcggcgtc gtcgatccgg taggtcaggt tggagtgccc
     1261 gccggcgagg cgctcgaagc gcagcggtgc gacggtgccg acgtgctcgg cgagccactc
     1321 ggtgacgggc cccacccgga tgcccgccgg gtcggctccg gtctcggcgt cgggctcggg
     1381 ctggccgggg tcctgggtca cggtcgaaac ctactcacgc ctcccggatc cgggccgccc
     1441 tgacgggact cggtggcggg tgcgccgata cggtctgccg cgggtgcggg ccccccgggt
     1501 gcaaccgggc agcgacggac tggagaagcc aatggacggc gagcgcgccg cgaacgtcag
     1561 cgtccgggtc ggcggtcaag ccgtcctcga gggtgtgatg atgcgagccg gacagcgggc
     1621 ctgcgtggcc gtgcgccggc ccgacggcga gatcgacacc cggatgctcg acgtgccggg
     1681 ctgggcccac cgggccgagt cgatcccgtt cgtgcggggc ctggccgggc tgggggagtc
     1741 cctggtggtg ggagtggagg cgctgcggtg gtccgagggc gcagccgggt tccggccgag
     1801 cgaccggacg cccgcaccca agtggctgct ggtcagcatc gccctcgtcg ccgtcgtcgt
     1861 gctgatcgtc gtgctgccca cggggctggc cggccttgtc gccggcggca cggcgtggtt
     1921 cgcggcggtc gaggccgcgg cgcgcatcac cgtcctcggc acctacgtgg ccctggtcgg
     1981 ccgccgtcgc gacgtgcggc gcgtgttcga gtaccacggt gccgagcacc tggtcgtgtc
     2041 cgggtacgag gaccggaagg tgctgcgtcc ggaccagctg cgaggtgggt cgatccacca
     2101 cccccgctgc gggacgagct tcttgttggt cgtggcggcc atcgccgccg tcgtccaccc
     2161 gttcctgccg cacggttcgc tcgtgctgca gctggccggt cgcatcctgg cggtcccgct
     2221 catcgtggcg gtgtcctacg aggtgctgac cgcgctcggc cgggtggccg cgctccggcc
     2281 cgggagccgg ctcgatcgaa tcctggtgtg gccgcagcgc ttcaccaccc gccgaccaga
     2341 tgatgcccag gtcgaggtgg ccatcgcggc cctccaggga gcactcggcc cggtcagcgt
     2401 ggtgccgacc gggccagggg tgctcgctgc atcgcggtcc tagaagatca ggatcaggat
     2461 gaggatgagg aggatgaggc cgccgattcc gatgtacatg tgaagtcctt tcgaggtggg
     2521 tggggacgcc cgaacccgta cccctggcgg gcgctcggca gaacgaccac gcgtggaacc
     2581 acccgttcgg gccagcccgc gccggggttc ccgtgccatg acgaagcgga atagggtccg
     2641 ctccatgcct gcctccaagc ctcccgtcac cgtcgccgtc accggtgcag ccggccagat
     2701 cggctactcg ctcctcttcc gcatcgcgag cggtgccctg ctcggcccgg accagccggt
     2761 gcacctcaag ctgctcgaga tcaccccggc catgggcgcg ctcgagggcg tggtcatgga
     2821 gctcgacgac tgcgccttcc cgctgctcgc cggctacacc acctccgacg atcccaacgt
     2881 ggccttcgac ggcgcccagg tcgccctgct cgtgggttcg atgccccgca aggccggcat
     2941 ggagcgggcc gacctgctcg aggccaacgg cggcatcttc accgtccagg gcaaggcgct
     3001 caacgacaac gccgccgacg acgtgaaggt gctggtggtc ggcaacccgg ccaacacgaa
     3061 cgccctcatc gccatgcact cggccccgga catcccgcgc gagcgcttca cggccatgac
     3121 ccgcctcgac cacaaccggg ccatcgccca gctggccaag aaggccggcg ccaccgtgaa
     3181 cgacatccgc aagatgacga tctggggcaa ccactcggcc acccagtacc ccgacgtgtt
     3241 ccacgccgag gtcgacggca agaacggctt cgaggccgcc ggctccgacc agtcctggct
     3301 ggagagcgat ttcatcccca ccgtgcagaa gcgcggcgcc gccatcatcg acgcccgggg
     3361 ttccagctcc gcggcctcgg ccgccaacgc cgccatcgac cacgtgcacg actgggtgct
     3421 cggctcgccc gagggcgact gggtctccat ggccgtgccg tccgacggct cctacggcgt
     3481 gcccgagggc atcatctcca gcttcccgtg caccaccgag gacgggcggt ggagcatcgt
     3541 gcagggcctc gagatcgacg acttcagccg ggcccgcatc gacgcctccg ccggcgagct
     3601 cgtcgaggaa cgcgacacgg tcgcgagcca gggcctcatc tgaacgagga aatcccgtgg
     3661 attcgacacc acgggtggtt cgaatccacg ggatttccgt tcacttgagg aacttggacg
     3721 tggtgttgtc ggcgagcacc ttgccgccgg tctggcaggt cgggcagtag tgcaccgtgt
     3781 agcgggtgta ggccacctcc cggatggtgt ctccgcacac cgggcaggcc tcgccggtgc
     3841 ggtggtgcac ggcgccgggg cggtccttgg aggacgacat gtcgtcgcgg gtgcgctcgt
     3901 aggcgaggcc ctcgtcgacg gccccggtga tggcagcgac gagcgcggcc gcgtcgtggg
     3961 gcccgagctt ggcggtcatc gcgaacggtg acagcttcgc ccggtgcagc acctcgttgg
     4021 ccaggcgtcg ccccagcccg gcgaggcggc gctgatcccg gaggaagccg tgcacgcggg
     4081 tgttctcctc gtggaggagg gcgagcatgg cgtcggcgtc gacctcggtg gcgtcggggc
     4141 cgaggtggtc gaggggctcg tggtcctcgg ggtcgccgtc gacgacccac accccggcct
     4201 tgcgctcggt gccggcctcg gacagcagca gtgcccggcc gtcgtcgaag cgccagcggg
     4261 cctggccgcc cttgggcttg gccgcctgct tcgggtcggg ccgcagccgc ccgccctgca
     4321 tcaggtgcac gacgtgggtg gcggacggga accgcagcag gagcagcttg ccccgccggc
     4381 ccaccgactc gagcggctcg ccgtgggtga cggccgggtc gggggagaag gtcttgaggg
     4441 cggtgaaggc gatgggctgg aaccgctcca ggacggcgcc ggcgaactcc tcggtcagcc
     4501 gctcggcgtg ggcctggacc tcggggagct cgggcacgtc aggagaacac cgtctcggcc
     4561 ttgacctcgg cgaacaggtc gccgagcacc tcgagggcgg cccggttgtc gagcagcgcc
     4621 cgcacgtcgc cgcccagcac caggtcgccg gcgatgaagg cgtcgagggc gggtcggcgg
     4681 ccggtggcga tgtccacggc ggtctcccgg tcgctggtca ggcgcacgtc gggctccccg
     4741 tcgcccccgg ccgtcaccgt gcagcggccc cggtccacga ccaggcgcca ccggggcccg
     4801 tcgcgcacga cctgctcgat ggtgacggtc gcgtcggcga gggggtccgg gtcggggacg
     4861 ggtcgggcgc gggcggcacc gtccatggcg gcgagccact cgtcggagag gaactgcacg
     4921 tcctgcatcg tggcacgcga cgatggcccc gtggcccccc gacccgccga cgagcacccc
     4981 cacgagccgg tggacgggga ctggagcgag acctggtcct tcgacttcgc ctcggccgac
     5041 ggcgagctcg gcgggtgggc cgccctcacc cgattcaccg accacgcctg gtaccaggca
     5101 gcgctcaccg gtcctcgccg ccagctgctc atggtgctcg accaccacgt gccgttccgc
     5161 agcaacccca tggaggtccg caccacgggg ctgtgggccg accacatctg cgagacgccc
     5221 ttcgaccact ggacactggg gctggagacc ttcgcgctgg gcgtggacga cccccgggag
     5281 ctgcacggtc gccagcacgg cgacaacgtg gcgctcggct tcgacctgga gtgggagacc
     5341 gacgggccgg tcgccgagca cccccagccc gccggggtgc acggctacga gctggcctgc
     5401 cgggtgtacg gcgaggtgct ggtgggccgc gacgtgatcg acttcgaggg cgtcggcagc
     5461 cggtcacacc actggggacc cctcgactgg gtcgtcgacg acggctggca cctgagcggg
     5521 gtggtggagg gcccgacccg ctggtcggtc gaccaccggg acgggacgac caccgcgctc
     5581 ctcgagaccg acggcaccac gcggcgggtg gcggcgagcg gcgaggtgga gatcgacgag
     5641 gacgggctcc cggtggccgc tcgggtcgag gtcgacggca ccgagctgcg ggtcgaggtg
     5701 ctggctccga cgccggtgcc catcgagctc cgggaccgcc agatccgtcg tcctcgtgcc
     5761 ctgtgccggt tgcgctcgtc cggcgggtgg accggcgtcg gatggctcga actgcgggcc
     5821 ggcgccggag ggtcgctcgg cgccgggtga gaggtggtca gcgggccggg acctcggcat
     5881 gctcgacggc ctcgctggcg cccgccaggc gggccacgat gacgatggcg gcgccggcca
     5941 ggagggcgag ggcgatcggc caccacacgt caccggcagg gagggcgagc tcggcaccgt
     6001 tggtggactc ctcgctgccg gtcccgttgg gccacggcca cagcacccgc agcgacccga
     6061 acatcagccc gatgagcccg gcgaggatcg gctgctcgtg gcgctccagg gcccagctga
     6121 gcagggacga gaacagcgcc agccccagca cggcgccggt gccgaagacg gcgatgacca
     6181 cgaggtcccg ctcgtcgacc gagtcgagga cgaactggta gaggccgagc atcagcagca
     6241 ggaacgagcc ggagatgccg ggcaggatca tggcgcagat ggcgatgctg cccccgacga
     6301 acacgaacgc caggggcggg tcctggatct ggcccgaccc gagcccgagg accaggaacg
     6361 tggccacggc cacgacggcc gcggtcgtga cgttgcgggc gtcccagcgg tgcacgaggt
     6421 ggacggtgga gaacagggac ccgacgacca ggccgaagaa gacggcggcg gtgcggatcg
     6481 gctcctcctc caggaagtgg ctgatgacgc tggccaggga gaccagggcc acgccgatgc
     6541 ccagcagcaa cgggagcagg aaccgccact cgaccttgcg gagctcctcg acgaagccac
     6601 cgggccgccc ccgcaccagc tgtccgagag cgcgggcgcc ctggcggatc tgggcgacga
     6661 gcgtggcgta gatgccgaag acgagggcca cggtgccgcc ggacacgccg gggacgacgt
     6721 cggcggcgcc catgcagaag cctcggaaga cctgcatggg gagctgggag cggaatcgca
     6781 cggcggaacg ctagcgaccg gcaccccggg ggcccgaagg cgggtcaggc cggcgtgacg
     6841 gggcggagcg cgagctcgaa ggcgtgagcg acgcggtcgg cccagacggc gtagccggcg
     6901 cccgaggggt ggtagcggtc accggagagg aagtgcgccc gatggcgccg ctcgatccac
     6961 ggcggccgct cccagacgtt ggcgtagtgg atgcggtcgc tggaccgggc cgcgtcctgc
     7021 agcacccggt cgagccagcg gccccgcaca gacgccaggg cgcggagcgg gtgggggagg
     7081 cggatggcgt cggccaggcc ggggatggag acgacgatca gcggcgcatc ggccgcggcg
     7141 gcgatggtgg cgatgttgcg gcggaagcgg cgccggctcg tgaacgcagc gatgtcgttg
     7201 gcgccgacgg tcaccccgac ggcatcgatg cgctggccga tctggccgag gccgtggcgc
     7261 agctgcagca gcgccggcag ctggacgtcg acgacgtcat tgctgttcgc ccccggcgtc
     7321 gcgaacacgt ggagccgggt gccccgttcg ctgtgggagg cgacgaggcg gggaagggtg
     7381 acgtcgggga cgatggcccc gaggccggca gccaggctgt cgcccagcca caccacccgg
     7441 tgcgccgacg ggtcggccgg gccgacgtcg ccgtcgagcc agtggaccgg atacgacgcc
     7501 gcgccgccga tcccggccat ccgggcctgg acgtaggcga gcagcccgac cccgccgacc
     7561 gcgctcgtgc ccgcagccgc gacggcggcc gcgtgcttca ggtaccggaa ggggtggcgg
     7621 ggcatccgtt ccgaggctac ggcgagatca gcgctgtccg gcgagcttgc ggttgcggag
     7681 ctgggcccgg acgtagtcgc ggttcatgaa cgcgatgtag tcgagcggga tctccttggg
     7741 gcacgccgcc gtgcactcgc cgtggttcgt gcaggagccg aagtactgct ccatggtg
//
LOCUS       DATISU010000018         3680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_280234, whole genome shotgun sequence.
ACCESSION   DATISU010000018 DATISU010000000
VERSION     DATISU010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280234"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VLR27_00935"
     CDS             <1..609
                     /locus_tag="VLR27_00935"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HSP02033.1"
                     /translation="ATFLDLDHGTYPYVTSSNPVAGGATVGSGIGPRHVDRVVGVGKA
                     YLTRVGSGPFPTELFDDLGDRIVDLGHEYGVNTKRRRRPGWFDAVMMRHAVRLNSLSE
                     LAITKLDVLDDFDTIKVCVAYELDGERIERMPDNQSVIHKCTPIYEELPGWKTDVSAS
                     TEPHHLPANARTYIEFLAEQCGVPISLLGVGPSRDQYVQLAS"
     gene            785..1645
                     /locus_tag="VLR27_00940"
     CDS             785..1645
                     /locus_tag="VLR27_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02034.1"
                     /translation="MWTGLVLVLLGASTVVAAPVGAGEESSGNLTCEAVIGDLGLTDV
                     LMAKDDVQRTAPYEGTAVNGDFVVTYEFEAAPEGAPEAFVVTFTSDDLVFGVFVKQAA
                     EKHELSRDAVFPAGTLTGSVYVPDGEFSHVSFCTGTVPTTEPPVTEPPTTEPPTTEPP
                     TTEPPTTEPPTTEPPTTEPPTTEPPTTEPPETPTTEPPVTEPPETPTTDVGGEVVVPT
                     PEPEAEPEAEAAPEPEAAPTVLGDTVVRSLPRTGSATVELGVVGVVMVLLGSALLALG
                     GRDLRNQAAG"
     gene            1755..2990
                     /gene="purD"
                     /locus_tag="VLR27_00945"
     CDS             1755..2990
                     /gene="purD"
                     /locus_tag="VLR27_00945"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HSP02035.1"
                     /translation="MKVCVVGSGGREHALAVVLARTADVVVTPGNPGIPGSVDTPPGD
                     IDADLYVIGPEQPLVDGLADQLRGAGKLVFGPGADGAKLEGSKAFMKEVLAAAGVPTA
                     RYADFDDPDEAIAFLDTLPAGPDGGYVIKTDGLAAGKGVLVTTDRAEAEADIREKLAG
                     ESFGDAGRRVVIEERMVGPELTVLAVCDGQRAVALAPSQDYKRVGDGDAGPNTGGMGC
                     YSPVPAADAGVVQTVLDDFVAPTLAELRRRGIDFRGVLYAGLMLTPEGPKLVEYNVRF
                     GDPESQVVLPRLTSDLAQLLAEAAAGELRSEPTFTDDACVAVACAAEGYPTSTRTGDV
                     IEGLDRLDDLDGVEVLFAGVAAGDEPGTLVTAGGRVLYVCGTAPDIDQARALAYQGIA
                     RISWPGMHHRSDIAAVPTT"
     gene            complement(3042..3563)
                     /gene="purE"
                     /locus_tag="VLR27_00950"
     CDS             complement(3042..3563)
                     /gene="purE"
                     /locus_tag="VLR27_00950"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HSP02036.1"
                     /translation="MTDTTGQPVVGIVMGSDSDWPVMEQAALALDELGVAYEADVVSA
                     HRMPDDMIEYGRTAADRGLRVIIAGAGGAAHLPGMLASVTVLPVVGVPVPLKHLDGMD
                     SLLSIVQMPAGIPVATVSIAGARNAGLLAARILAAGTDAAAMDLRERLATFADELRDL
                     AVEKGRRLRERGE"
BASE COUNT          523 a         1390 c         1266 g          501 t
ORIGIN      
        1 gccaccttcc tcgacctcga ccacggcacg tacccctacg tgacgtcgtc gaacccggtg
       61 gccggcggcg cgacggtcgg ctcgggcatc ggtccccgcc acgtcgaccg ggtcgtcggc
      121 gtcggcaagg cgtacctgac cagggtcggt tccgggccct tccccaccga gctgttcgac
      181 gacctcggcg accgcatcgt cgacctcggt cacgagtacg gcgtgaacac caagcgccgc
      241 cgccggccgg gctggttcga cgcagtgatg atgcgccacg ccgtgcggct caactcgctg
      301 tccgagctgg ccatcaccaa gctcgacgtg ctcgacgact tcgacaccat caaggtctgc
      361 gtggcctacg agctcgacgg cgagcgcatc gagcggatgc ccgacaacca gtcggtgatc
      421 cacaagtgca cgcccatcta cgaagagctc cccggctgga agaccgacgt gtccgcgtcg
      481 accgagcccc accacctgcc ggccaacgcc cgcacctaca tcgagttcct cgccgagcag
      541 tgcggcgtgc cgatcagcct cctcggcgtc ggtccgtccc gggaccagta cgtccagctc
      601 gccagctgat cccgctgcac gctccgtagg tattccgcta cccctcagga cctttcggcc
      661 actcccggtg accggctcct ctactcgctc tgcgtggttc ctgttccact gtcagtgacc
      721 ggaaatcccg gccaccgtca ggaatcgagg agcgatgaca cgccatcgtt cgaccaagag
      781 gaaggtgtgg acagggctgg tgctggtgct gctcggcgcc tccacggtgg tggcagcccc
      841 cgtcggtgcc ggtgaggagt cctccgggaa cctcacctgc gaggccgtga tcggcgacct
      901 cggcctcacc gacgtgttga tggccaagga cgacgtgcag cggacagctc cctacgaggg
      961 caccgccgtc aacggcgact tcgtggtcac gtacgagttc gaagcggcac ccgagggtgc
     1021 tccggaggcc ttcgtcgtga cgttcacctc ggatgacctc gtgttcggcg tcttcgtcaa
     1081 gcaggccgcg gagaagcacg agctgagccg agacgcggtg ttcccggccg gcacgctgac
     1141 cggttcggtc tacgtccccg acggcgagtt cagccacgtg agcttctgca ccggcacggt
     1201 cccaacgacc gaaccgccgg tgaccgagcc gccgacgacc gagccgccga ccacggagcc
     1261 ccccacgacg gagcccccca cgacggaacc gcccacgacg gaaccgccca cgacggagcc
     1321 gccgaccacg gaaccgccca cgacggaacc gccggagacc ccgacgaccg agcccccggt
     1381 gacggagccg ccggagaccc cgacgacgga cgtcggcggt gaggtggtgg tccccacccc
     1441 ggagcccgaa gcggagccgg aggccgaggc ggcccccgag cccgaggcgg ccccgacggt
     1501 cctcggtgac acggtggtcc ggtccctgcc ccgcaccggg tccgccacgg tggagctcgg
     1561 cgtcgtcggc gtggtgatgg tgctgctcgg cagcgccctc ctcgccctcg gcggccggga
     1621 cctgcggaac caggcagccg gctgagcgcc ggcacccagc acagctggct cgaggggccg
     1681 gcggaggatc tccgccggcc ccttccgcgc gccccttcgg gggccgctcc gggccgcgtc
     1741 taacgtcgcc gtccgtgaag gtctgcgtgg tcggttccgg tggtcgtgag cacgcgctgg
     1801 ccgtggtgct ggcccggacg gccgatgtcg tcgtcacgcc cggcaacccc ggcatccccg
     1861 gctcggtcga caccccgccc ggggacatcg acgccgatct gtacgtcatc gggcccgagc
     1921 agcccctcgt cgacggcctc gccgaccagc tccgcggtgc cggcaagctc gtgttcggcc
     1981 ccggcgccga cggcgcgaag ctcgagggct ccaaggcgtt catgaaggag gtcctggccg
     2041 cagccggcgt cccgaccgcc cgctacgccg acttcgacga ccccgacgag gcgatcgcct
     2101 tcctcgacac cctccccgcc gggccggacg gcggctacgt catcaagacc gacgggctcg
     2161 cggccggcaa gggcgtgctc gtcaccaccg accgggccga ggccgaggcc gacatccgcg
     2221 agaagctcgc cggcgagagc ttcggcgacg ccgggcgccg ggtggtcatc gaggagcgca
     2281 tggtcggccc cgagctcacc gtgctcgccg tctgcgacgg ccagcgtgcc gtcgccctgg
     2341 ccccgtccca ggactacaag cgcgtcggcg acggcgacgc cggaccgaac accggtggca
     2401 tgggctgcta cagcccggta ccggctgcgg acgccggggt cgtgcagacg gtgctcgacg
     2461 acttcgtcgc ccccaccctg gccgagctgc ggcgccgcgg catcgacttc cgcggggtgc
     2521 tctatgccgg cctgatgctc acccccgagg ggccgaagct ggtggagtac aacgtccgct
     2581 tcggcgaccc cgagtcccag gtcgtcctgc cccgcctcac cagcgacctg gcccagctgc
     2641 tggccgaggc cgcggccggt gagctgcgca gcgaaccgac cttcaccgac gacgcctgcg
     2701 tggcggtcgc gtgtgccgcc gagggctacc ccaccagcac gcggacgggc gacgtgatcg
     2761 aggggctcga ccgcctcgac gacctggacg gggtcgaggt gctgttcgcc ggcgtcgccg
     2821 ccggcgacga gccgggcacc ctggtcaccg ccggcggtcg ggtcctctac gtgtgcggca
     2881 cggcgccgga catcgaccaa gctcgtgctc tcgcctacca aggcatcgcc cgcatctcct
     2941 ggccgggcat gcaccaccgc agcgacatcg cggccgtccc caccacctga acgcagcacc
     3001 acggtgttcc gtggtcgctg ccagttcctc gcggaggtgc gctactcgcc tcgctcgcgg
     3061 aggcggcggc ccttctcgac ggccaggtcg cgcagttcgt cggcgaacgt cgccagtcgc
     3121 tcccgcagat ccatggcggc ggcgtcggtg ccggcggcga ggatcctcgc ggcgagcaga
     3181 ccggcgttgc gggccccggc gatggacacg gtggcgaccg ggatcccggc gggcatctgg
     3241 acgatggaca gcagcgagtc catcccgtcg aggtgcttca gcgggacggg gacgccgacg
     3301 acgggcagca cggtgaccga ggcgagcatg ccgggcaggt gcgcagcacc gccggcgccc
     3361 gcgatgatca cccggaggcc ccggtcggcg gcggtgcggc cgtactcgat catgtcgtcg
     3421 ggcatccggt gggccgacac cacgtcggct tcgtacgcca cgccgagctc gtcgagcgcc
     3481 agcgccgcct gctccatgac gggccagtcg ctgtcgctgc ccatgacgat gcccacgacg
     3541 ggctggccgg tggtgtcggt catccgttcg ctcctcgaag ctggtcggcg gcaccgcggg
     3601 cccgcagtcg gaggtcgcgg aggtcctcac cgcagagcgt gacgtggccg atcttgcggc
     3661 cgggtcggac ctgcttgccg
//
LOCUS       DATISU010000019         6339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_606276, whole genome shotgun sequence.
ACCESSION   DATISU010000019 DATISU010000000
VERSION     DATISU010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606276"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VLR27_00955"
     CDS             <1..868
                     /locus_tag="VLR27_00955"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HSP02037.1"
                     /translation="LRVDTMMLHVHDDTDEAIRTFYPSSEEPHRDPEELDLDVGDAHW
                     VSATRSGFVQATDIDSLLEVAEREDLVVRIEARAGDQVVIGTPLATVWGRGGRPLDAV
                     SPETQDAIRDVVRLGYERTLDQDSGFGFRQLEDIAVKAMSPSINDPVTAAHAVGHMAD
                     LIVGLLGCRLGPTLHVDGQGEGRAIVPDRDLRYYLDLMCAQLRRFAATEPSVLASLLR
                     ALRDIGLSCRDDHQRDEVRRAADLVVASMDQGVIEPDAAFVQEHRDRVEAAIAGDRLG
                     AYADRAGEARSM"
     gene            complement(961..1362)
                     /locus_tag="VLR27_00960"
     CDS             complement(961..1362)
                     /locus_tag="VLR27_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02038.1"
                     /translation="MHEARRSIDGLDKLPRLHCRTMSSAANIRPAHSTGSGANWSRLH
                     LSASPSTIPFVKAFNLVSAVVTATVLAVATLQLTLPGQEGTPGWRIAAAWLLAALVWA
                     VIYSFVRDGIRTTLSALVPHRRTDHRGASPT"
     gene            complement(1355..2053)
                     /locus_tag="VLR27_00965"
     CDS             complement(1355..2053)
                     /locus_tag="VLR27_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02039.1"
                     /translation="MLDEIADLVCAHGGPRTLVGIDGRSGAGKSTFADELAVRLAARG
                     LAAVRSTTDSFHRSRGERLSRGATSGDGYYLDSHQLDVIVDELLEPFAQGAANVRTAA
                     FDEPSDTPVDTVVAVEPVAVLVFDGLFLQRIELADHWDITVFLDADGRRDREWLSFLL
                     DELPDGDSARAATLDARLTRARWPRYRDGWRTYVDRVNPASAATLLVDNNDFGRPTLL
                     RTRREDGSDDAERA"
     gene            complement(2239..2514)
                     /locus_tag="VLR27_00970"
     CDS             complement(2239..2514)
                     /locus_tag="VLR27_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02040.1"
                     /translation="MAKAGAWLIAVGLSLGALSTALYVARQGELTQGRVPFDASPYVI
                     WGGIAGVAVTCGTVLIAAAQVIVALGTDDAPPPRTRESTNGPRSHYE"
     gene            complement(2524..3051)
                     /locus_tag="VLR27_00975"
     CDS             complement(2524..3051)
                     /locus_tag="VLR27_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02041.1"
                     /translation="MLALVLAVVLSFTYVPSGAQAWDDPTLGGGTAWTPWHRWTTVLW
                     MLAAGASIVTLVVLTVVSDATRRGAVTALGCAVVTGIATVVALATRGLVQWDQLALWA
                     VAVGGGLDGYWPALDDGVRFVLIEGTEVDPGDYGAAVAVHLLAHAVGVAAAAGTLALV
                     VRRWRLSPASDPAVE"
     gene            complement(3205..4743)
                     /locus_tag="VLR27_00980"
     CDS             complement(3205..4743)
                     /locus_tag="VLR27_00980"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSP02042.1"
                     /translation="MTAARPTESLLAPNDPRQLGPWKLLSRLGSGGMGAVYLGTSEGV
                     LAAIKVIHPGLAQDPTFTRRFAREIAVSSRVAGPHVAKVLDADPDGDPPWLATEFIDG
                     PTLTDGIEADGPLRGTSLDAFAAALISALQSIHAAGIIHRDLKPSNVILTPDRPVVID
                     FGIASASDETSFTQTGTALGSAGWMAPEQVEGKRAGTAADVYAWAGTVAFAATGRPPF
                     GTGPAHAVLYRVVHHEPDLAGIQQPLAGLLERALAKDPQDRATVDSIARAYSHDGTIA
                     DFVAGTWVAPPEAAQTRVHPTPTSVELASEPTGGPARRILWPAAIAVVAAVFIALIAF
                     AVRDNEGDTAASTDTVIDTTTTTPPATTTTSAPEDTTTTTATTTTTSTVPTTTSPPAP
                     AEPVFSSAFGAATPDALGLLDFFSEHELETVRVNATVHDLSPEDLYRMDDTDVLFVPG
                     TCDQFCEGDAYELLFRELDSVPDAAVYYDSGTIEIKGRFVVNSVQIQTGGVFSVALRA
                     VPIA"
     gene            4873..4946
                     /locus_tag="VLR27_00985"
     tRNA            4873..4946
                     /locus_tag="VLR27_00985"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4907..4909,aa:Arg,seq:cct)
     gene            complement(4956..5831)
                     /locus_tag="VLR27_00990"
     CDS             complement(4956..5831)
                     /locus_tag="VLR27_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP02043.1"
                     /translation="MGVPGHLLHLFREAPSKRRLLRSLALGGRGGDRTVTSADGTRLS
                     VRRSGDGVPVVLVHGTLDGISAFSLIELTIAERHAVWVYDRRGRGGSGDQAEYSLQRE
                     IEDLEAVLAAAGSPAHVVGHSFGALIALAAAASGTAMRSLVLYEPPLRQDQLDHRVIT
                     QVEALVACGDLDRAITVMATELAGVSEDEIAVPRRIKPMWNQLRDGVGATPRELEVVS
                     DVRWDRFDLPVRDRPVLVIRGDRDHAPAYPRPEDLPRFVTDPEITTLPGQGHLATTFA
                     PARFAEVVLDFIDRH"
     gene            5981..>6339
                     /locus_tag="VLR27_00995"
     CDS             5981..>6339
                     /locus_tag="VLR27_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP02044.1"
                     /translation="MVMVEGEVQAARRVGFVAELERHGDAVAVVDRDGSTCTYADLAS
                     RADAVAARLGERRRLVLVEAVNDLDGIVAYLGALRGGHAVLLASPGDGRSADLVSTYR
                     PDVVIGGGLAIAEQRTGT"
BASE COUNT         1016 a         2115 c         2254 g          954 t
ORIGIN      
        1 cctgcgcgtc gacacgatga tgctccacgt ccacgacgac accgacgaag cgatccgcac
       61 cttctacccg tcatccgagg agccccaccg ggacccggag gaactcgacc tcgacgtcgg
      121 tgacgcccac tgggtgtccg caacgcgcag cggcttcgtc caggccaccg acatcgactc
      181 actcctggag gtggcggagc gtgaagacct cgtcgtccgc atcgaggcgc gcgccgggga
      241 ccaggtcgtg atcgggacgc cgctcgccac ggtctgggga cggggtggca ggccgctcga
      301 tgcggtctct cccgagaccc aggatgcgat ccgtgacgtg gtgcggctcg gctacgagcg
      361 cacgctcgac caggacagcg gcttcgggtt ccgccagctc gaggacatcg cggtgaaggc
      421 gatgtccccg tcgatcaacg acccggtcac cgccgcccac gccgtcgggc acatggccga
      481 tctgatcgtc gggctcctcg gatgccggct cggcccgacc ctgcacgtcg acggccaggg
      541 cgagggccgc gccatcgtgc ccgaccgtga tctgcgctac tacctcgacc tgatgtgcgc
      601 ccaactccga cggttcgcgg ccaccgagcc gtcggttctg gcttccctac tgcgcgcact
      661 gcgcgacatc ggcctctcct gtcgggacga ccaccagcgc gacgaggttc gccgggcggc
      721 ggacctcgtg gtggcctcca tggaccaagg cgtcatcgaa cctgacgctg ccttcgtgca
      781 ggagcaccgc gaccgggtcg aggctgccat cgccggggac cggctcggcg cctacgccga
      841 ccgtgcgggc gaggcccgct ccatgtgaac cgaccagcgc cgccggagac accgccgatc
      901 aagagccact cgaacacgcc cggggtggcc agctgggcga ctctcgcctg ttcgacttca
      961 tcaggtaggc gaggcacctc ggtggtcggt gcgtcggtgt gggaccagcg ccgacaaggt
     1021 ggtccggatg ccgtctcgca cgaagctgta gatgacagcc caaaccagcg ctgcgagcag
     1081 ccacgccgcg gcgatgcgcc acccgggggt cccctcctga cctggcagcg tcaactgcag
     1141 cgtcgccacc gcgagcaccg tggcggtcac caccgccgag accaagttga acgccttcac
     1201 gaagggaatc gtgctgggag acgctgacag gtgcaaccgg gaccagttgg cgccggatcc
     1261 cgtggagtgc gccgggcgga tgttcgccgc cgatgacatc gtgcggcaat ggagccgtgg
     1321 gagcttgtcg agaccgtcga tggatctgcg ggcttcatgc acgttcagca tcgtcgcttc
     1381 cgtcttcccg acgggtgcga agcagtgtgg gacgtccgaa gtcgttgttg tctacgagca
     1441 gggtggcagc ggacgccggg ttcactctgt cgacgtaagt gcgccaaccg tcccgatagc
     1501 gcggccagcg ggcgcgggtg agacgggcgt cgagcgtggc ggcgcgggcg ctgtcgccat
     1561 cgggcagctc atcgagcagg aagctgagcc actcccggtc gcggcgcccg tcggcatcga
     1621 ggaagaccgt gatgtcccag tgatctgcga gctcgatacg ttgcaggaag aggccgtcga
     1681 aaacgagaac ggcaacgggt tcgacggcca caacagtgtc gacaggggtg tccgagggct
     1741 catcgaaggc tgcagtacgc acattcgctg cgccctgggc gaacggttcc aggagctcgt
     1801 cgacgatgac atcgagctgg tgggagtcca ggtagtagcc gtcgccagat gttgcgcccc
     1861 gggagagccg ctcgccgcgg gatcggtgga aggagtcggt ggtcgagcgg accgctgcaa
     1921 gtcccctcgc cgcaaggcgt accgccagct cgtcagcgaa agtcgacttc cccgcacccg
     1981 aacgcccgtc gatgccgacg agcgttcgtg gtccaccgtg agcgcacacg agatcggcga
     2041 tctcgtcgag cacacgatcg cgttgcggtg tgaccaccgg gtcgtggaac ccgtcaagtc
     2101 cggccggcac agcacccagc ctgccgcacc gcgagcacga gctgcggcgt cgatgacggt
     2161 gacctgtgcc cgatccgttg cgaaggccag cccgtgaggt cagctccagc gtgcaccgca
     2221 tcggtgcctg acaaggggct actcgtagtg actgcgcggt ccgttcgtgc tctcgcgtgt
     2281 ccggggtggc ggtgcgtcat cggtcccaag cgcgacgatc acctgggccg ctgcgatgag
     2341 gaccgtgccg cacgtgacgg ccacgccggc aatgccaccc cagatcacgt acggcgatgc
     2401 gtcgaacggg acacggccct gggtgagctc accctgccga gccacgtaga gcgcagtcga
     2461 gagcgctccg agactgagcc cgactgcgat caaccacgcg cccgccttgg ccaccgcgac
     2521 ggtctactcc acggccgggt cgctcgccgg cgagagccgc cagcggcgaa ccaccagggc
     2581 gagggtcccg gccgccgccg ctacgccgac ggcgtgggcg agcaggtgca cggccacggc
     2641 ggcgccgtag tcgccggggt cgacctcggt cccttcgatc agaacgaacc gcacaccgtc
     2701 atcgagggca ggccagtagc catcgagacc accaccgacg gcaaccgccc acagcgccag
     2761 ctggtcccac tggaccaatc cgcgagtcgc aagcgcaacc acggtcgcga tccccgtcac
     2821 gactgcacag cccagggcgg tgaccgctcc gcggcgagtg gcatcggaaa cgacggtcaa
     2881 gacaacgagg gtgacgatgg aggcgccggc ggcgagcatc cacagcacag tcgtccaccg
     2941 gtgccacggc gtccacgccg tcccgccacc gagggtcgga tcgtcccacg cctgcgcgcc
     3001 ggacggcacg tacgtgaacg acagcacaac ggcgagcacg agcgcgagca cggcaagagc
     3061 cgcggcggca ccagtcacga cgacagcggt ccggtgggca gtcgtcggcg gcacccgagg
     3121 agcatgtcag ccgttgagcg gtcgtcgggt catccgagac gcggtcgctc ggcttccgaa
     3181 gcgtgacctg gtcgcctgac ttcctcatgc aatgggcacg gcgcgcagtg cgacggagaa
     3241 gactcccccg gtctggatct gcacgctgtt cacgacgaag cggcccttga tctcgatcgt
     3301 tccgctgtcg tagtagacgg cagcgtcagg aacggaatcg agttctcgga acaggagctc
     3361 gtacgcatcg ccttcgcaga actggtcgca agttccaggg acgaacagaa cgtcggtgtc
     3421 gtccatgcgg tacaggtcct caggggacag gtcgtgaacg gtcgcgttga ctcggacggt
     3481 ttcgagttcg tgctcgctga agaagtcgag cagtcccagc gcatccggcg tcgctgcgcc
     3541 gaaggcggag ctgaagactg gttctgccgg tgccggtggc gatgtcgtcg tgggaaccgt
     3601 ggacgtcgtc gtcgtggtcg cggtcgtagt ggtcgtgtct tctggagcgg acgttgtggt
     3661 ggttgcgggt ggggtcgtcg tggtcgtgtc gatgaccgtg tcggtggacg ccgccgtgtc
     3721 gccttcgttg tcgcgaacgg cgaaggcgat cagcgcgatg aagacggcag ccacgacggc
     3781 gatcgccgct ggccacagga tccgcctggc tggcccgccg gttggctcgc tggcgagctc
     3841 caccgaggtt ggcgttgggt gcactcgggt ctgtgcggct tcgggtgggg cgacccaggt
     3901 gccagcgaca aagtcagcaa tcgtgccgtc gtggctgtag gcccgtgcga tcgagtcaac
     3961 cgtcgcccgg tcttgtgggt ctttcgcgag cgccctctcg agcagaccgg cgagcggctg
     4021 ttggatccca gcgaggtcgg gctcgtggtg gaccactcgg tagagcacgg cgtgggcagg
     4081 tccagttccg aagggtgggc ggccggtcgc agcgaaggcg accgtgcctg cccaggcgta
     4141 gacgtctgcc gctgtgccag cacgtttgcc ttcgacctgt tctggggcca tccatcctgc
     4201 cgagcccagc gccgtgcccg tctgcgtgaa ggatgtctcg tctgaagctg aagcgatgcc
     4261 gaagtcaatg acgaccgggc ggtcgggggt gaggatgacg ttggacggct tcaggtcacg
     4321 gtggatgatg cctgctgcgt ggatggattg gagcgcagag atcagcgcag ctgcgaaggc
     4381 atcgagggat gtgccgcgta agggcccgtc ggcctcgatg ccgtcggtga gggtcgggcc
     4441 gtcgatgaac tcagtggcca accacggagg atcgccatct gggtcagcgt ccaacacctt
     4501 cgcgacgtga ggtcctgcga cgcgggacga gacggcgatc tcgcgggcga accggcgagt
     4561 gaaggttggg tcctgcgcaa gacctggatg gatcaccttg atcgctgcga gcacaccctc
     4621 gctcgtgccc aggtagacgg cacccatgcc cccggacccc aaacgactga gcagcttcca
     4681 cggcccgagc tgtctcgggt cgttcggcgc caggagagac tcggtgggtc gggcagcggt
     4741 catgacgagc aatggactgt aggagtccgt gtgctcagaa cctcggagta tccgaactta
     4801 gaagaggccg caactaccct cctgtgctga cctagacgcg gaactccacg cggagctcca
     4861 cgcggaagtc ccgccctcgt agctcagtcg gatagagcga ccgcctccta agcggtaggc
     4921 cacaggttcg attcctgtcg ggggcacgcg acgcctcagt ggcggtcgat gaagtcgagc
     4981 accacctcgg cgaaccgggc cggagcgaac gtcgtggcca ggtgcccctg ccccgggagc
     5041 gtggtgatct cggggtcggt gacgaagcgg ggcaggtcct ccgggcgcgg gtacgccggc
     5101 gcgtggtcgc gatcgccccg gatgaccagc accggacggt cgcgcacggg caggtcgaag
     5161 cggtcccagc ggacgtcgga gaccacctcg agctcgcggg gggtggcccc gaccccgtcg
     5221 cggagctggt tccacatcgg cttgatccgg cgggggacgg cgatctcgtc ctcgctcacg
     5281 ccggcgagct cggtcgccat gaccgtgatc gcacggtcga ggtcgccgca ggccacgagc
     5341 gcctcgacct gggtgatgac ccggtggtcg agctggtcct ggcgcagcgg tggttcgtag
     5401 agcacgagcg accgcatggc agtgccggat gccgctgcgg cgagggcgat cagcgcgccg
     5461 aaggagtggc cgaccacgtg ggccggcgaa ccggcggcag ccagcaccgc ctcgaggtcc
     5521 tcgatctccc gctggagcga gtactcggcc tggtcgccgc tcccgccccg gccccgccgg
     5581 tcgtacaccc acacggcatg gcgctcggcg atcgtgagct cgatcaggga gaacgcgctg
     5641 atcccgtcga gggtcccgtg gacgagcacc accgggacgc cgtcgcccga ccgccgcacg
     5701 gacaggcggg tcccgtcggc cgaggtgacc gtcctgtcgc caccccggcc ccccagcgcc
     5761 agcgaccgga ggagacgccg cttgctcggc gcctcgcgga acaggtgcag caggtgaccg
     5821 ggtacgccca tcgagcctcc ctcgactcgg ccgaacggcg gtcgggccgg tcggcgccgg
     5881 tgacccgacg gtaccgcgct ccaggagtcg gtgggagggc aggttccgct cctccgctct
     5941 ggttaggcat gcttacccta tgtcattagg gtgactcggc atggtcatgg tcgagggtga
     6001 ggtgcaggcg gcgcgccggg tcgggttcgt cgccgagctc gagcggcacg gcgacgccgt
     6061 cgcggtcgtg gaccgggacg ggtccacctg cacctatgcc gacctggcct cgcgagcgga
     6121 tgccgtggcc gcgcggctgg gtgagcgccg ccggttggtg ctcgtcgagg cggtcaacga
     6181 cctcgacggc atcgtcgcct acctgggcgc cctgcgcggc ggccacgcag tgctcctcgc
     6241 cagccccggc gacggacggt cggccgatct ggtctcgacc taccggcccg atgtcgtcat
     6301 cggcggaggt ctcgccatcg ccgagcagcg aacgggcac
//
LOCUS       DATISU010000020         5273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_280361, whole genome shotgun sequence.
ACCESSION   DATISU010000020 DATISU010000000
VERSION     DATISU010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280361"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /gene="atpD"
                     /locus_tag="VLR27_01000"
     CDS             <1..835
                     /gene="atpD"
                     /locus_tag="VLR27_01000"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HSP02045.1"
                     /translation="KIDENTSVLDKAALVFGQMDEPPGVRRRVALSALTSAEYFRDER
                     NQDVLLFIDNIFRFVQAGSEVSTLLGRMPSAVGYQPTLADEMGTLQERITSTKGRSIT
                     SLQAVYVPADDYTDPAPFTTFTHLDATTELSRDIAALGIYPAVDPLSSTSTILSPEVV
                     GERHYNVARRVQETLQRYKELQDIIAILGLDELSEEDRITVARARKIQRFLSQPMFVA
                     EVFTSLPGVFTPAEETVDSFEQLINGELDDLPEQAFLNVGGADDARRKAEQLRKSTGG
                     E"
     gene            841..1257
                     /gene="atpC"
                     /locus_tag="VLR27_01005"
     CDS             841..1257
                     /gene="atpC"
                     /locus_tag="VLR27_01005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HSP02046.1"
                     /translation="MPMQVELVSPERILWSGQAEMVIARTLGGGDIAFLPGHAPFVGA
                     LGTGIVTIRPSSGGADEKVACHGGFVEVSGDPESSHVTILSDVAELSSQIDVERAREA
                     KERAEVALRHEHDTEAEAALRRAHARLEAAGQATNH"
     gene            1286..1804
                     /locus_tag="VLR27_01010"
     CDS             1286..1804
                     /locus_tag="VLR27_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HSP02047.1"
                     /translation="MPRRRFAVALVIPPPVSVEIDGLRRALGDRQLGRIDPHITIIPP
                     INLHEDQIGEALAVVQAAARVAQPMALTLGPVTTFAETSPVRFLAVDPWEPVVELYRS
                     CWTDVLERPEKRPFHPHCTVDIDGGPTDGPDPAVELLAAYQVEVELDRVTVLENVDRG
                     WDPYIAYRFGLG"
     gene            complement(1806..2795)
                     /gene="glpX"
                     /locus_tag="VLR27_01015"
     CDS             complement(1806..2795)
                     /gene="glpX"
                     /locus_tag="VLR27_01015"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HSP02048.1"
                     /translation="MRQPQAPDRNLALELVRVTEAAALAASRWMGRGDKEGADGAAVD
                     AMRIVLGSVPMDGIVIIGEGEKDEAPMLYNGEHIGDGNPPKVDIAVDPIDGTTLTALG
                     RSGAISVIAMSEQGSMFDPGPCMYMEKIATGPEARDAIDLNKSPTENLKAVAAAKGDA
                     VRDLTAVILDRDRHKDLIAEVREAGARVRLIPDGDVAGAISTCWPQSGADILFGIGGT
                     PEGVITAAALKAMGGAHLGRLWPRNEAERKAAIDAGYDLDRVLTEDDLVRSDNCFFAA
                     TGITDGELLRGVHYDNRGASTQSLVMRSKSGTVRLIEAQHKIDKLADYASVDF"
     gene            2911..3675
                     /locus_tag="VLR27_01020"
     CDS             2911..3675
                     /locus_tag="VLR27_01020"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSP02049.1"
                     /translation="MINTKRQPPVSTTTLPVLMEAIADRDATVASVSAVVGHDPGLAA
                     RVLALANSSQFGLCRRVTDLAQAVTIVGTGVVQTLAIANTAALVDTSGIIADAHDHAI
                     RVAIAARMLAPAAGVDPDDAFAAGLLHDIGELLLLQDSPSEYASLHRTFATHADQLRA
                     EKRAFGTDHALAGAEHLLDWRVPDVIADAVADHHDPFASSDPTTIVVAAADELISAET
                     GRHHALDLLELDVAAADGLRSRVATEAGTLIGLVGT"
     gene            3685..4287
                     /locus_tag="VLR27_01025"
     CDS             3685..4287
                     /locus_tag="VLR27_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02050.1"
                     /translation="MLNEPGQDDHDGELELDTSALDGPDPRAEVGDGSDDCPPLDDAK
                     EQVGYELFEWDPTELDELDDVLHELGLPHEWVADGYEVVVHVEDEARVDALLPTIRFP
                     DELPAEVDDGDDTDVQVLGDLFTAADRLHGGASTDAVNGFLDAAERIGERPPYGLDDK
                     EWGQVVDQVDEIIDLFHAAGPAEEITEAAYRLRRRLRPLV"
     gene            4304..5029
                     /locus_tag="VLR27_01030"
     CDS             4304..5029
                     /locus_tag="VLR27_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02051.1"
                     /translation="MDALAELAIYHSRPFAPTRRLALAPSHLPVDPAPGPGGVLLGGI
                     VAHHRNELDEDLFESLLKLTLQLQEGQRIVQPRMRHRFQDDRHGLTRSRARLRREDGG
                     LVIDFENKGTGLQLMLGAVYTAGSLEPGPRRAVMDAVRKGLRWGGPLDRRLYAHLSGL
                     DGGQEWSADVYEDPVRWALQTLGMRAATDGSAADADMPDRKQVQRLFRDALRAAHPDH
                     GGATDDAAKRINDLTEARRILLS"
     gene            5026..>5273
                     /locus_tag="VLR27_01035"
     CDS             5026..>5273
                     /locus_tag="VLR27_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase"
                     /protein_id="HSP02052.1"
                     /translation="MSPKKKPARPAAAAGLLVTPGASADRDNRTLVALDEGLDVPVER
                     ITIRGRAVRKVHEQLREEMEDFADRLGVDTAELAIGGRS"
BASE COUNT          752 a         1980 c         1810 g          731 t
ORIGIN      
        1 caagatcgac gagaacacgt cggtcctcga caaggccgcc ctcgtgttcg gccagatgga
       61 cgagcccccg ggcgtgcgcc gccgcgtcgc cctctcggcc ctcaccagcg ccgagtactt
      121 ccgtgacgag cgcaaccagg acgtgctgct cttcatcgac aacattttcc gcttcgtgca
      181 ggccggttcc gaggtctcca ccctcctcgg ccgcatgccg tccgccgtgg gctaccagcc
      241 caccctcgcc gacgagatgg gcaccctcca ggagcgcatc acctcgacca agggccgctc
      301 gatcacgtcg ctgcaggccg tgtacgtgcc tgccgacgac tacaccgacc cggcgccgtt
      361 caccaccttc acccacctcg acgccaccac cgagctctcc cgtgacatcg cggccctcgg
      421 catctacccc gccgtggatc cgctttcgtc gacctcgacc atcctctccc ccgaggtggt
      481 cggcgagcgc cactacaacg tggcccgccg ggtgcaggag accctgcagc gctacaagga
      541 gctgcaggac atcatcgcca tcctcggcct cgacgagctc tccgaggaag accgcatcac
      601 ggtggcccgg gcccgcaaga tccagcggtt cctgtcccag ccgatgttcg tggccgaggt
      661 cttcaccagc ctccccggcg tgttcacccc cgccgaggag accgtcgact ccttcgagca
      721 gctcatcaac ggcgagctgg acgacctgcc cgagcaggcc ttcctgaacg tcggcggcgc
      781 cgacgatgcc cgacgcaagg ccgagcagct gcgcaagtcc accggcgggg agtgatcggg
      841 atgccgatgc aggtcgagct ggtctcgccc gagcgcatcc tgtggtccgg tcaggccgag
      901 atggtcatcg cccgcaccct gggcggcggc gacatcgcgt tcctgcccgg gcacgccccc
      961 ttcgtcggtg cgctcggcac cggcatcgtc acgatccgcc cctcgagcgg tggggccgac
     1021 gagaaggtgg cctgccacgg tggcttcgtc gaggtctccg gtgacccgga gtcgtcccac
     1081 gtcacgatcc tctccgacgt cgccgagctc tcgagccaga tcgacgtcga gcgggctcgc
     1141 gaggccaagg agcgggccga ggtggcgctg cgccacgagc acgacaccga ggccgaggcc
     1201 gcgctgaggc gggcgcatgc ccgcctcgag gccgccggcc aggccaccaa ccactgagcc
     1261 gctgagcggg cggagcgctg acgacgtgcc ccgtcgtcgc ttcgccgtcg ctctcgtcat
     1321 cccgccgccg gtctcggtcg agatcgacgg cctgcggcgc gccctcggcg accgccagct
     1381 cggccggatc gacccccaca tcacgatcat cccgccgatc aacctccacg aggaccagat
     1441 cggcgaggcc ctcgccgtgg tgcaggccgc cgcccgcgtc gcccagccca tggcgctgac
     1501 cctcgggccg gtgacgacct tcgccgagac ctccccggtc cggttcctgg ccgtggaccc
     1561 gtgggagccg gtggtcgagc tgtaccggtc gtgctggacc gacgtgctcg agcggccgga
     1621 gaagcgcccg ttccaccccc actgcaccgt cgacatcgac ggcgggccca ccgacggtcc
     1681 ggacccggcg gtcgagctgc tcgccgccta ccaggtggag gtcgagctgg accgggtgac
     1741 ggtgctggag aacgtggacc ggggctggga cccctacatc gcctaccgct tcgggctggg
     1801 ctgaatcaga agtcgacgct ggcgtagtcg gcgagcttgt cgatcttgtg ctgggcctcg
     1861 atgaggcgga ccgtgcccga cttggagcgc atgaccagcg actgggtcga ggcgccgcgg
     1921 ttgtcgtagt ggacgccacg cagcagctcg ccgtcggtga tgccggtggc ggcgaagaag
     1981 cagttgtcgc tgcgcaccag gtcgtcctcg gtgaggacgc ggtcgaggtc gtagccggcg
     2041 tcgatggccg ccttgcgctc ggcttcgttg cggggccaga ggcgaccgag gtgggcgccg
     2101 cccatcgcct tgagggcagc ggcggtgatg acgccctccg gcgtgccgcc gatgccgaag
     2161 aggatgtcgg cgccggactg gggccagcag gtggagatcg cgccggccac gtcgccgtcg
     2221 gggatcagcc gcacccgtgc acctgcctcg cgcacctcgg cgatgaggtc cttgtgccgg
     2281 tcccggtcga ggatgaccgc ggtgaggtcg cgcaccgcgt cgcccttggc ggcggcgacg
     2341 gccttgaggt tctcggtggg gctcttgttc aggtcgatgg cgtcgcgcgc ctcgggaccg
     2401 gtggcgatct tctccatgta catgcacggg ccggggtcga acatcgagcc ctgctcggac
     2461 atggcgatca ccgagatcgc cccgctgcga cccagggcgg tgagcgtcgt gccgtcgatc
     2521 gggtcgacgg cgatgtcgac cttgggcggg ttgccgtcgc cgatgtgctc gccgttgtag
     2581 agcatcggcg cctcgtcctt ctcgccctcg ccgatgatga cgatgccgtc catggggacc
     2641 gacccgagca cgatgcgcat ggcgtccacg gcggcgccgt cggcgccctc cttgtcgccc
     2701 cgccccatcc agcggctggc ggcgagggcg gcggcttcgg tgacgcggac gagctcgagg
     2761 gcgaggttgc gatcgggagc ctggggctgg cgcatgtcgc gaaccgtacc cgcggcgcga
     2821 gccggaggcg gtagcccgtt cgaccacgga gagtgctcaa gatcgatggt tcgactgccg
     2881 atggtgctgc atgcgtcagg aggagccggc atgatcaaca cgaagcggca gccgccggtg
     2941 tccaccacga ccctcccggt cctgatggag gccatcgccg accgggacgc gaccgtcgcg
     3001 tcggtgtccg ccgtcgtcgg tcacgacccc ggcctcgccg ctcgggtgct ggccctggcc
     3061 aactcgtcgc agttcgggct gtgccgccgg gtgaccgacc tggcgcaggc cgtcacgatc
     3121 gtcggtaccg gcgtcgtgca gacgctggcc atcgccaaca ccgccgcctt ggtcgacacc
     3181 agcgggatca tcgccgacgc ccacgaccac gccatccggg ttgccatcgc cgcccggatg
     3241 ctggcgccgg ccgccggtgt ggaccccgac gacgccttcg ccgccggact gctccacgac
     3301 atcggcgagc tgctgctcct ccaggacagc ccgagcgagt acgccagctt gcaccggacg
     3361 ttcgcgaccc acgccgacca gctgcgcgcc gagaagcgcg ccttcggcac cgaccacgcc
     3421 ctcgccggcg ccgagcacct cctcgactgg cgggtccccg acgtcatcgc cgacgccgtg
     3481 gccgaccacc acgacccctt cgcctcctcg gatccgacca ccatcgtcgt ggccgcggcg
     3541 gacgagctca tctccgccga gacgggacgg caccacgccc tcgacctgct cgagctggat
     3601 gtggcggccg cggacggact gcggtcccgg gtcgccacgg aggccgggac gctgatcggg
     3661 ctggtcggca cgtagcctgc gagggtgctc aacgagcccg gacaggacga ccacgacggc
     3721 gagctcgagc tggacacgtc cgcgctcgac ggtcccgacc cccgcgccga ggtcggcgac
     3781 ggcagcgacg actgcccacc gctggacgac gccaaggagc aggtcggcta cgagctgttc
     3841 gagtgggacc ccaccgagct ggatgagctc gacgacgtcc tccacgagct cggcctgccc
     3901 cacgagtggg tcgccgacgg ctacgaggtc gtcgtgcacg tcgaggacga ggcgcgcgtc
     3961 gacgccctgc tgcccaccat ccgcttcccc gacgagctcc cggccgaggt cgacgacggc
     4021 gacgacaccg acgtgcaggt cctcggcgac ctgttcaccg ccgccgaccg gctccacggc
     4081 ggggccagca ccgatgcggt gaacgggttc ctcgacgccg ccgagcgcat cggcgagcgc
     4141 ccgccctacg ggttggacga caaggagtgg ggccaggtgg tcgaccaggt cgacgagatc
     4201 atcgacctgt tccacgccgc cggccccgcc gaggagatca ccgaggccgc ctaccgcctg
     4261 aggcgacggc tgcgccccct ggtgtgaccg gtagcgtcga tccatggacg ccctggccga
     4321 gctggcgatc taccactccc ggcccttcgc tcccacccgg cggctcgccc tcgcgccgag
     4381 ccaccttccc gtcgacccgg cccccggtcc cggcggcgtg ctgctcggcg gcatcgtcgc
     4441 ccaccaccgc aacgagctcg acgaggacct cttcgagagc ctgctgaagc tgaccctgca
     4501 gctgcaggag ggccagcgca tcgtgcagcc ccggatgcgg caccggttcc aggacgaccg
     4561 ccacgggctc acccgctccc gggcccggct gcgccgcgag gacggcgggc tggtcatcga
     4621 cttcgagaac aagggcacgg gcctgcagct gatgctcggc gcggtctaca ccgccggcag
     4681 cctggagccc ggcccccggc gggcggtcat ggacgcggtc cgcaagggac tgcggtgggg
     4741 tggcccgctc gaccggcggc tctacgccca cctgtccgga ctggacggtg gtcaggagtg
     4801 gtccgccgac gtctacgagg atccggtccg ctgggcgctc cagaccctcg gcatgcgggc
     4861 cgccacggac ggaagcgccg ccgatgccga catgcccgac cgcaagcagg tgcagcgcct
     4921 gttccgcgac gccctgcgcg ctgcgcaccc cgaccacggc ggtgccaccg acgacgccgc
     4981 caagcgcatc aacgacctga ccgaggcccg gaggatcctg ctgtcgtgag cccgaagaag
     5041 aagcccgctc gccccgccgc cgccgccggg ttgctcgtca cccccggcgc cagcgccgac
     5101 cgagacaacc gcaccctggt cgccctcgac gagggcctcg acgtgccggt ggagcggatc
     5161 accatccggg gccgggccgt gcgcaaggtg cacgagcagc tgcgggagga gatggaggac
     5221 ttcgccgacc gcctcggcgt cgacaccgcg gagctggcga tcggcgggcg ctc
//
LOCUS       DATISU010000021        15428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_699615, whole genome shotgun sequence.
ACCESSION   DATISU010000021 DATISU010000000
VERSION     DATISU010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 15428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15428
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699615"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VLR27_01040"
     CDS             <1..327
                     /locus_tag="VLR27_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HSP02053.1"
                     /translation="DGVNDAAALAQADLGLSMGTGTDVAIEASDITLVRGDLRSAADA
                     IRLARRTLGTIKGNLFWAFAYNVAALPLAAAGLLNPMLAGAAMAFSSVFVITNSLRLR
                     RFRAAS"
     gene            403..909
                     /locus_tag="VLR27_01045"
     CDS             403..909
                     /locus_tag="VLR27_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HSP02054.1"
                     /translation="MPRLTAHDSTTAPRHAGERLAALEARHGDVAVMTRTMAGSASLL
                     IGYLELSKAMKRSRLDRGVAERMSIAVQQELGCATCLDAHVHAARAAGVPDYEIDLAK
                     QATSADGRTAALVAFAHRVHTDPSSITATDLDALRDLGVTETEILDTVGLVALNVLTG
                     TLNLVAGL"
     gene            complement(937..1674)
                     /locus_tag="VLR27_01050"
     CDS             complement(937..1674)
                     /locus_tag="VLR27_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HSP02055.1"
                     /translation="METELLYLRDAHLRAFEARVTEVDDEGGRVALDRTAFYPTGGGQ
                     PHDTGTLETPAGHRTVTSVRKEGGVVWHALEGGDLPGVGDPVTGQLDWQRRHDLMRTH
                     TALHVLCGVIWNEWGVPVTGGNMEPLAARMDFEFDPLPDGFKERVEDLVNIELAADRP
                     IEVSFLPRSVAVQDEDLIRTKVNLIPESVPEIRVVDIVGLDKQADGGTHVAHTGEVGR
                     IRITKTESKGKGNKRIRLEVVDAPGGM"
     gene            1743..3119
                     /locus_tag="VLR27_01055"
     CDS             1743..3119
                     /locus_tag="VLR27_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSP02056.1"
                     /translation="MPSPHRRPRWHTSLLAALVLAAACTSGGDEDAGPDTTESTVAVT
                     TRCPDGTEIPDAAPAGATPEIDATSAGPEPRDGALDIGTLLPRSGDLAFLAPAAIAGV
                     ELAVDDITAAGGVLGAPVGLRHGDSADGTPGVVDEEAARLLGAGVDVLIGPLSSSAAA
                     SVLPQVVAADAVLVSPAATSGGLDALDEAGRLFRTAPTESLQGRALADLIRSDGHRTV
                     SIAARDDEHGRTIADGLVERLARTGGAVASRVDHDPASDDLGTTVVEQLDLTADAVVL
                     VGLAETALILDALVDAGEGPRERAVYGTDGNLGERLGDLVEDRADLACMRGLLPVARA
                     DAELADRIRSHDPALAEVGDAALDLAAESYDAVVLAALAAAAAGSDAGGEIAAAMAPV
                     SDGGTTCREPATCLDLLAAGTDVSYVGRTGPIALDEAGNRSDAGLTVVAFDGDGHLAR
                     LAAHRLGS"
     gene            3215..4555
                     /locus_tag="VLR27_01060"
     CDS             3215..4555
                     /locus_tag="VLR27_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSP02057.1"
                     /translation="MRKPGKFRLLVALFLSLSLVAAACGDDDDEATDTTDDTADTEET
                     TETDETDETTEEASGGSDCEVGEGDGVLKIGTVLPETGNLAFLGPPEFAAAELAVQDI
                     NAAGGVLDADVELFQGDSGDTSTDVASQTVDRHLNEGVDAFIGAASSGVSFTFIDKVT
                     GACKIQFSPANTSPDFTDYDDNGLYFRTAPSDVLQGRVLADLVVADGNESATVMALQD
                     PYGEGLLQYTLEPLEEQGVVVDEDFVYDPFAENFDAEVQRVVDADSDALVLIGFDESA
                     RILTGLFENGFTPESKSIYLVDGNVGNALGEQVSASMVGVKGTLPAAEITDEFRSRLL
                     EVDPELEDFSYAPETYDAVVITALAALVAESDDAVAIAAEINGVTRDGETCTTFEECA
                     GMIAEGTDIDYDGPSGPQTFGEAGEPSEASFAILQYGEGNTIDDSLTEYRFAQL"
     gene            complement(4714..5448)
                     /locus_tag="VLR27_01065"
     CDS             complement(4714..5448)
                     /locus_tag="VLR27_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP02058.1"
                     /translation="MATGEPLLIADELVAGYLPGVNILNGCNITVHDGELVGIIGPNG
                     AGKSTCLKAVFGLIPVRDGNVTLRGEDIANLKAHELVERGVGYVPQNNNVFPRLTVRE
                     NLEMGVYLRPKSFKDSFARVVDMFPRLGERKDQRAGALSGGERQMLAMGRALMMEPSV
                     LLLDEPSAGLSPALQDDVFLRCRRINEAGVSILMVEQNASKCLQICDRGYVLDQGANA
                     YTGSGRELLEDPKVVELYLGTLAANR"
     gene            complement(5448..6302)
                     /locus_tag="VLR27_01070"
     CDS             complement(5448..6302)
                     /locus_tag="VLR27_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP02059.1"
                     /translation="MVTDAFDQVEPRPGSPKRDTILSVDGLVRRFGGITAVDVEHLEI
                     QANAITGLIGPNGAGKTTFFNLITGFDEITAGTFQFKGESLEGVPAYRVARKGLVRTF
                     QLTKALSKLSVIDNMKLGAVGQVGENPFKALIRPIWGNQEREIEARADELLKRFKLYD
                     KRDDYAGELSGGQRKLLEMARALMADPEVICLDEPMAGVNPALTQSLLQHVKELKDEG
                     LTVIFVEHDMDVVRDISDWVVVMAEGAIIAEGSHASIGKNQAVIDAYLGTSHEIDLGT
                     IDLDPEKR"
     gene            complement(6292..7281)
                     /locus_tag="VLR27_01075"
     CDS             complement(6292..7281)
                     /locus_tag="VLR27_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSP02060.1"
                     /translation="MNWMGILESTLEAMVTKNAVVFALAAIGLNIHYGYTGLLNFGQA
                     GFMAAGAYGTGIAITQWSWPIVPAAGFGLVLSVVLALLLGLPTLRLRADYLAIVTIAA
                     AEIIRRVVKSTEYRGVTGGSGGINGIGRGFYDNNPIDSGLDLGIVDFSARQLWFMVVG
                     WTIVALCMVLTYLLVRSPWGRVLKAIREDEEAVRSLGKNVTWYKMQSLILGGLFGALG
                     GIMLTLEQQSVQPDQFFTPTTFYAFTALILGGAGRVFGPVVGSMLFWGIMQFTSVVLR
                     QGIRNGHIPESVMDGVQAGQVPLIVAGVLLGALMVFRQQGIFGNKREVALDGH"
     gene            complement(7281..8654)
                     /locus_tag="VLR27_01080"
     CDS             complement(7281..8654)
                     /locus_tag="VLR27_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSP02061.1"
                     /translation="MSAITRRLVLLLVALTSVIGFSFVGAAVASAQEATEEPGDPEAN
                     PEAQEAADQDIESVIGSLKYDDAEDNEVPVEGVEISATNADGEEVASDTTAEDGTFLL
                     ELPEPGEYTISVDPETIPEGVSLRSEERGSTTFELSPGQQRNVLFPLVEGEGGGSERT
                     GPSRLDRVPQLAFEGFKFGLIIAMCAVGLSLIFGTTGLVNFAHGELVTFGALLAFLFN
                     VTFGIHLLIAAPIAVVLSGAFGALFDRGFWRPLRNRGTSLIALMVVSIGASLALRHMF
                     LYQFGGRTRPYDDYAIQTDTIDFLNITMVRKDIWIVAVSIIRLVLVALALERTRIGKA
                     TRAVADNKDLAESSGIDVEKVILVVWAAGTALAAVGGIFLGLTEQVSWLGGFQLLLLM
                     FAGVVLGGLGTAYGALVGSIVVGLAVQLSVLFISSELKNVAALVILALVLLVRPQGIL
                     GRKERVG"
     gene            complement(8720..9040)
                     /locus_tag="VLR27_01085"
     CDS             complement(8720..9040)
                     /locus_tag="VLR27_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HSP02062.1"
                     /translation="MDRALMGETDHDPTTATGRRHLVVTLQLPDRPGALGSVASRIGA
                     LGADITDVRVSSRRAGVAEDVFHLDLPAVEGVDTVGLLLAEIGHVDGVVAPSVSYPSP
                     GCCG"
     gene            complement(9111..9812)
                     /locus_tag="VLR27_01090"
     CDS             complement(9111..9812)
                     /locus_tag="VLR27_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HSP02063.1"
                     /translation="MSDAPATPAPSSPADGQAVLHLFFALTPLADADAVSEAVASAEA
                     DGYQVVPVAVLGHKADIGFMALGADQWRLRLLQTELVSAGLELVDSYVSLTEISEYAQ
                     GVPDQMKQARLFPQLPPEGKPAFCFYPMSKRRTVEGNWYTLPYEERMELMMEHGKSGR
                     TFAGRVLQVVTGSTGVDDFEWGVTLFAQKPDDLKEVVYTMRYDRASAIYAEFGTFITG
                     MTGSLPEVLDAVGLR"
     gene            complement(9848..10642)
                     /locus_tag="VLR27_01095"
     CDS             complement(9848..10642)
                     /locus_tag="VLR27_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HSP02064.1"
                     /translation="MTRGRTNRLLTTWVDADGRSRSPDEVAVEEPLEIRLDGHLVGTT
                     MRTPGHDFELAVGFCHTEGLLGDAPVRSAKYCRGEGPGAVVESEFNVVTVSTGGLAPV
                     PEPRLGVTSSACGMCGTTAVDELRTRIHPLPVGEPIPDDVLLAAPSELLGDTPIFAAT
                     GAVHAAALFSPTGEAVVVREDVGRHNAVDKVVGHQLLAGELPRHGFGLAVSGRVGFEI
                     VQKAWAAGITTVVAKGAPTSLAVEAARMARITLIGWATSAGGRAYD"
     gene            10735..12525
                     /locus_tag="VLR27_01100"
     CDS             10735..12525
                     /locus_tag="VLR27_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HSP02065.1"
                     /translation="MTDEPLRAARPLHRPHRRSRTALAAALLALLASIVAVPTAMAAP
                     SAPAEGVAVLRLDGRGHGHGVGLSQWGARSMAAQGADAATILAHYYPGTETGSAGGEV
                     VVGIASGARSTISLPQGGELRSARGGAQAEGFPIPLGPGEVVTIHHDGSGWRVERGGV
                     RGLDAGNAQMFQEADDCVLLCPPEDTPDPQPAPEPGTEPEPEPQPEPEPDDCALCGPP
                     EEQPDAQPAPQPAPGPQPGADQQAPRSPSPIWAVPVDGGTVHSVDRGRTYRGLLEVGG
                     GAGSVRARNHVDIEDYLRGMAEVPGTWPAAAVQAQTVAARTYALRAMAGAGEICDSES
                     CQVYAGVARESAGQDAAVAATRGWVLTHGGALAATFYSASAGGHSATVQEGFGSSYDI
                     PYLPARPEPTDDTRPWNLEVSLGDVAARLGYPGRLTDVRVDAAGPSGRPLAMTLVGDA
                     GERPVDPQVFRRRLGLRSTFFTVQSGVAGQAPPPPPAPVESDQLLTADAVVSDDVGPV
                     RPARARSMVVASPRSLTPADGLAAARTGQVAVLALLAAAATWFAVTRLHAVGRTTSSR
                     GLGTAPLGAAPIGWREQLAATMTPWRNHNP"
     gene            12504..12929
                     /locus_tag="VLR27_01105"
     CDS             12504..12929
                     /locus_tag="VLR27_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02066.1"
                     /translation="MEEPQPLTATRVVLRGLLRRCPLCGSTGIFESWFRQVERCPRCN
                     YPTTRVEDQWIGSLGMNIIVSFTLLLGVIAVGFAVTYPDPPVGVLLVLAVSVAALFPV
                     FFFPISRSLWSAIDLAMRPPDPDDDVDPRFLPPTRHGPG"
     gene            complement(12997..13656)
                     /locus_tag="VLR27_01110"
     CDS             complement(12997..13656)
                     /locus_tag="VLR27_01110"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSP02067.1"
                     /translation="MTTTRIVLVRHGEAQSHVEGVVGGRRGCTGLSELGRRQATALRD
                     RLDRTAELGEVHALYASTLPRASETASIIAPAIGGLDVVERDDLREFDIGPEGDGMAW
                     EELEQRFPTPDVWTPFHHHVPGAETWAGFAARVGGALDDIARTHRGETVVVACHGGVV
                     EHSFTAFHGRGMTGWLGSVLIQNCGVTEWQHQERPEMPWVKERTWSLVRHNDHSHVPT
                     A"
     gene            complement(13738..15363)
                     /gene="groL"
                     /locus_tag="VLR27_01115"
     CDS             complement(13738..15363)
                     /gene="groL"
                     /locus_tag="VLR27_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799237.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HSP02068.1"
                     /translation="MAKLIEFNEEARRALERGMNTLADAVKVTLGPKGRNVVLDKKWG
                     APTITNDGVSLAKEIDLEDPYERIGAELVMEVAKKTDAVAGDGTTTATVLAWAMVREG
                     LRNVAAGANSMSLKKGIEQAVEAAVASIKDQAVEVDSKGQIAQVASISSADEEIGTMI
                     SEAIDKVGKDGVITVEESQTFGMDMDLVEGMRFDKGYISPYFVTDQDRMEAVLDDAYI
                     LLVSSKITAVRDLLPVLEKVMQSGKPLAIIGEDIEGEALATLVVNKIRGTFKAVAVKA
                     PGFGERRKALLQDMAILTGGQVISEEIGLKLENTTLDLLGTAAKIVVTKDETTIVDGG
                     GSDDDLQGRIGQIKAEIDNTDSDYDREKLQERLAKLSGGVAVLKVGAATEVELKEKKH
                     RIEDAVSTTKAAIEEGVVAGGGTTLLRAKAKVDQLVGDLVGDEATGAKIVAHALQEPV
                     KQIARNAGLEGGVVVDRVLAMSGSTEGLNAATGNYEDLTKAGIIDAAKVTRSALQNAA
                     SIAALFLTTEAVIADKPEKDGAGGGMPGGMDDF"
BASE COUNT         2205 a         5595 c         5388 g         2240 t
ORIGIN      
        1 gacggggtca acgacgccgc cgcgctggcc caggccgacc tcgggctgtc gatggggacc
       61 ggcaccgacg tcgccatcga ggccagcgac atcaccctcg tgcgcggcga cctccgatcc
      121 gccgccgacg cgatccggct cgcccggcgc accctcggca ccatcaaggg gaacctgttc
      181 tgggccttcg cctacaacgt cgccgccctg cccctcgccg ccgcgggtct cctcaacccg
      241 atgctggccg gcgccgccat ggccttctcc agcgtcttcg tcatcaccaa cagcctgcgg
      301 ctccgacgct tccgcgccgc cagctgagct cccaggggcg ggcgggcaca ccttcccgcc
      361 cgcccccacg ccaaccggaa ccccgaacct ggaggtggct cgatgccacg cctgaccgcc
      421 cacgactcga caaccgcacc ccgccatgcc ggcgaacgcc tcgctgcgct cgaagcacgg
      481 cacggcgacg tcgccgtcat gacccgcacc atggccggat cggcctccct gctcatcggt
      541 tacctcgagc tgagcaaggc catgaagcgc tcccgcctcg accgaggtgt cgccgagcgg
      601 atgtcgatcg ccgtgcagca ggagctcgga tgcgccacgt gcctcgacgc acacgtgcac
      661 gccgcacggg cggccggggt gcccgactac gagatcgacc tggccaagca ggcgacctca
      721 gcagacggtc gcaccgccgc cctggtcgcc ttcgcccacc gcgtccacac cgacccgtcc
      781 tcgatcaccg ccaccgacct cgacgcgctg cgggacctgg gcgtcaccga gaccgagatc
      841 ctcgacaccg tggggctcgt cgccctgaac gtcctcaccg ggacgctcaa cctcgtcgcc
      901 ggtctctgac ctgcccagag gagcccccga cccgggctac atgccgccgg gcgcgtcgac
      961 gacctcgagc cggatgcgct tgttgccctt gcccttggac tcggtcttgg tgatgcggat
     1021 gcgcccgacc tcacccgtgt gcgcgacgtg ggtgccgccg tcggcctgct tgtcgaggcc
     1081 gacgatgtcg acgacgcgga tctcgggcac ggactcgggg atcaggttga cctttgtccg
     1141 gatcaggtcc tcgtcctgca ccgccacgga tcggggcagg aacgacacct cgatgggccg
     1201 gtcggcggcc agctcgatgt tgaccaggtc ctcgacccgc tccttgaacc cgtcgggcag
     1261 cgggtcgaac tcgaagtcca tgcgggcggc caggggttcc atgttccccc cggtcaccgg
     1321 cacgccccac tcgttccaga tgaccccgca gagcacgtgg agcgcggtgt gcgtgcgcat
     1381 gaggtcgtgc cggcgctgcc agtcgagctg acccgtgacc gggtcgccga caccgggtag
     1441 gtccccgcct tccagggcgt gccagacgac gccgccctcc ttgcgcacgg acgtgacggt
     1501 ccggtggccg gcgggcgtct cgagcgtccc ggtgtcgtgg ggctggccac cgccggtcgg
     1561 gtagaaggcg gtccggtcga gcgccacgcg accgccctcg tcgtccacct cggtgacgcg
     1621 agcttcgaag gctcgcaggt gggcgtcgcg caggtagagc agctcggtct ccatccacgc
     1681 gagtctgtcc catcgacacc gggccgatcc gacgtcgtcc gtcgtagcct ccgcccatcg
     1741 ccgtgccctc ccctcaccga cgaccgcggt ggcacaccag cctcctggcc gctctcgtgc
     1801 tggccgccgc ctgcacctcg ggcggcgacg aggacgccgg ccccgacacc accgagtcga
     1861 ccgtggccgt cacgacccgc tgcccggacg ggacggagat ccccgacgcc gcgccagctg
     1921 gcgcgacgcc ggagatcgac gcgacgtcag ccggtccgga acctcgcgac ggtgccctcg
     1981 acatcggcac cctcctgccc cgctccggcg acctcgcctt cctcgcccca gccgccatcg
     2041 ccggcgtgga gctcgccgtc gacgacatca ccgccgcggg cggggtcctc ggcgcccccg
     2101 tgggtctccg tcacggcgac tcggccgatg ggacgcccgg cgtggtcgac gaggaggccg
     2161 cccgcctgct cggtgccggg gtcgacgtcc tgatcggccc gctctcctcg agcgcggcgg
     2221 cgagcgtgct gccccaggtg gtcgcagccg atgcggtgct ggtgtcgccc gcggcgacct
     2281 ccggcgggct cgatgcgctc gacgaggccg gtcgcctctt ccgcaccgcg ccgaccgagt
     2341 cgctccaagg acgggcgctc gccgatctca tccggtccga cggccaccgg acggtctcga
     2401 tcgccgcccg ggacgacgag cacggccgga ccatcgccga cgggctggtc gaacggctcg
     2461 cccgcaccgg cggcgccgtc gccagccggg tcgaccacga ccccgcgtcc gacgacctcg
     2521 ggacgacggt cgtggagcag ctcgacctga cagccgatgc cgtcgtgctg gtcggcctcg
     2581 ccgagacggc cctgatcctc gacgctctcg tcgacgctgg cgagggtccc cgggaacggg
     2641 cggtctacgg caccgacggc aacctcggcg agcggctcgg ggacctcgtc gaggaccgcg
     2701 ccgacctcgc ctgcatgcgg gggctgctac ccgtcgcccg cgcggacgcg gagctcgccg
     2761 accggatccg gtcccacgac cccgcgctcg ccgaggtggg cgacgccgcg ctcgacctcg
     2821 cggccgagtc ctacgacgcc gtggtgctcg cggcgctcgc ggccgccgca gccggatcgg
     2881 acgccggagg cgagatcgct gctgcgatgg caccggtctc ggacggcggg accacctgcc
     2941 gggagcccgc cacctgcctg gacctgctcg ccgccggcac cgacgtctcc tacgtcggcc
     3001 ggaccggtcc gatcgcgctg gacgaggccg ggaaccggtc cgacgccggg ctcacggtcg
     3061 tcgccttcga cggcgacggc cacctggctc ggctggccgc tcatcggctg gggtcgtgag
     3121 ccgaaacccg gcattcacgt ccaacgcccc catcaccgag ccaggaccgg tactctctgc
     3181 ctccctgttg gtgaacacaa aggaaaccca tcacatgcgc aagccaggca agttccgcct
     3241 gctcgtggcg ctgttcctgt ccctgtctct cgttgctgct gcgtgcggcg acgacgacga
     3301 cgaggccacg gacacgaccg acgacacggc agacaccgaa gagaccaccg agaccgacga
     3361 gaccgacgag acgaccgaag aggcgtccgg cggttccgac tgcgaggtcg gcgagggcga
     3421 cggcgtgctc aagatcggca ccgtgctgcc cgagaccggc aaccttgcct tcctcggccc
     3481 gcccgagttc gcagctgcgg agctggctgt ccaggacatc aacgccgctg gcggcgtcct
     3541 cgacgccgac gtggagctgt tccagggcga ctccggcgac acgtccaccg acgtggcctc
     3601 ccagaccgtg gaccgtcacc tgaacgaggg cgtcgacgcc ttcatcggtg ccgcctcctc
     3661 cggcgtgtcc ttcaccttca tcgacaaggt gaccggcgcc tgcaagatcc agttcagccc
     3721 ggccaacacg tcgccggact tcaccgacta cgacgacaac ggtctgtact tccgcaccgc
     3781 tccgtccgac gtcctccagg gccgcgtcct cgccgacctc gtcgtcgccg acggcaacga
     3841 gagcgccacg gtcatggccc tgcaggatcc ctacggtgag ggcctgctgc agtacacgct
     3901 cgagcccctc gaggagcagg gtgtcgtcgt cgacgaggac ttcgtctacg acccgttcgc
     3961 cgagaacttc gacgccgagg tccagcgcgt ggtcgacgcc gacagcgatg ccctcgtgct
     4021 catcggcttc gatgagtcgg cccgcatcct gaccggcctc ttcgagaacg gcttcacgcc
     4081 ggagtccaag agcatctacc tggtcgacgg caacgtcggc aacgccctcg gcgagcaggt
     4141 ctcggcctcg atggtcggcg tgaagggcac cctgcccgcc gccgagatca ccgacgagtt
     4201 ccggtcccgc ctgctcgagg tcgaccccga gctcgaggac ttctcgtacg ccccggagac
     4261 ctacgacgcc gtcgtcatca ccgccctcgc tgccctcgtg gccgagagcg acgatgccgt
     4321 ggcgatcgcc gcggagatca acggcgtgac ccgcgacggt gagacctgca ccaccttcga
     4381 ggagtgcgcc ggcatgatcg ccgaaggcac cgacatcgac tacgacggtc cctcgggccc
     4441 gcagacgttc ggtgaggcgg gcgagcccag cgaggcgagc ttcgccatcc tgcagtacgg
     4501 cgagggcaac accatcgacg actcgctcac cgagtaccgg ttcgcccagc tctagacctg
     4561 agctgagcac cagaacctga ggaggccggg cccacgggcc cggcctcttc gcgtggtgcg
     4621 atcggcactg ccggcgagcg atcggccgcc gcgacgagcg cgacagcagc actgccagaa
     4681 cggaacgggt cgcccctcga agggggcggt ggttcagcgg ttggctgcga gcgtgccgag
     4741 gtagagctcg acgaccttgg ggtcctcgag cagctcgcga cccgaaccgg tgtaggcgtt
     4801 ggcgccctgg tccagcacgt agccgcggtc gcagatctgg aggcacttgc tggcgttctg
     4861 ctcgaccatg aggatcgaga cgccggcctc gttgatgcga cggcagcgca ggaacacgtc
     4921 gtcctgcagc gccggggaca gcccggcgga gggctcgtcg agcaggagga cgctgggctc
     4981 catcatgagc gcccggccca tggccagcat ctggcgctcg ccgccggaga gggcaccggc
     5041 gcgctggtcc ttgcgctcac ccaggcgggg gaacatgtcg accacgcggg cgaaggagtc
     5101 cttgaacgac ttcgggcgca ggtacacgcc catctccagg ttctcccgca ccgtgaggcg
     5161 cgggaacacg ttgttgttct ggggcacgta gcccacgccg cgctcgacga gctcgtgagc
     5221 cttcaggttg gcgatgtcct cgcctcgcag ggtcacgttg ccatcgcgga cggggatcag
     5281 cccgaacacc gccttcaggc aggtggactt gccggcgccg ttcgggccga tgatcccgac
     5341 gagctcgccg tcgtggacgg tgatgttgca gccgttgagg atgttgacgc ccggcaggta
     5401 gccggcgacc agctcgtcgg cgatgaggag gggttcgccc gtggccatca gcgcttctcc
     5461 gggtccaggt cgatggtgcc gagatcgatc tcgtgcgagg tgccgaggta ggcgtcgatg
     5521 accgcctggt tcttgccgat ggaggcgtgg gagccctcgg cgatgatcgc gccctcggcc
     5581 atgaccacga cccagtcgga gatgtcgcgg accacgtcca tgtcatgctc gacgaagatg
     5641 accgtgaggc cctcgtcctt gagctccttg acgtgctgga gcagcgactg ggtgagggcg
     5701 gggttcacgc cggccatggg ctcgtccagg cagatgacct cgggatcggc catgagggca
     5761 cgggccatct cgagcagctt gcgctgaccg ccggacagct cgccggcgta gtcgtcgcgc
     5821 ttgtcgtaca gcttgaagcg cttgagcagc tcgtcggccc gggcctcgat ctcgcgctcc
     5881 tggttgcccc agatcggtcg gatcagggcc ttgaacgggt tctcccccac ctggccgacg
     5941 gcgccgagct tcatgttgtc gatcaccgac agcttggaga gcgctttggt gagctggaac
     6001 gtgcggacca gccccttgcg ggccaccctg taggccggca cgccctcgag cgactcgccc
     6061 ttgaactgga acgtgcccgc cgtgatctcg tcgaagccgg tgatcaggtt gaagaacgtc
     6121 gtcttgccgg cgccgttggg gccgatcagg ccggtgatcg cgttggcctg gatctcgagg
     6181 tgctcgacgt cgacggcggt gatgccgccg aagcgccgca cgaggccgtc cacggacagg
     6241 atcgtgtccc gcttgggcga cccgggtcgg ggttcgacct ggtcgaaggc atcagtgacc
     6301 atcgagcgcc acctcccgct tgttgccgaa gatcccctgc tggcggaaga ccatgagggc
     6361 accgagcagc acgccggcga cgatcagggg gacctggccg gcctgcacgc cgtccatcac
     6421 cgactcgggg atgtggccgt tgcggatgcc ctggcgcagg accacgctgg tgaactgcat
     6481 gatgccccag aacagcatcg agccgacgac ggggccgaag acgcgcccgg cgccgccgag
     6541 gatgagggcg gtgaaggcgt agaacgtggt cggcgtgaag aactggtcgg gctgcaccga
     6601 ctgctgctcg agggtgagca tgatgccgcc gagggcgccg aacaggccac ccaggatcag
     6661 gctctgcatc ttgtaccagg tgacgttctt gccgaggctg cgcacggcct cctcgtcctc
     6721 gcggatggcc ttcagcacgc gcccccacgg gctgcgcacc agcaggtacg tgagcaccat
     6781 gcacagcgcc acgatggtcc agcccacgac catgaaccac agctggcgag ccgagaagtc
     6841 cacgatgccg aggtcgaggc cgctgtcgat ggggttgttg tcgtagaagc cgcgcccgat
     6901 gccgttgatg ccgccggagc cgcccgtgac gccgcggtac tcggtggact tcacgacacg
     6961 tctgatgatc tctgctgctg cgatcgtcac gatggccagg tagtccgccc gcaatcgcag
     7021 ggtcggcaga cccagcagca gggcgagcac gacggagagc acgaggccga acccggcggc
     7081 ggggacgatg ggccagctcc actgggtgat ggcgatgccg gtgccgtagg cgccggcggc
     7141 catgaacccg gcctggccga agttgagcag gccggtgtag ccgtagtgga tgttgaggcc
     7201 gatggcggcg agggcgaaca cgaccgcgtt cttcgtgacc atcgcctcga gggtggattc
     7261 gaggatgccc atccagttca tcagcccacc cgttccttcc ggcccaggat gccctggggt
     7321 cgcaccagca ggaccagcgc gaggatgacc agcgcggcga cgttcttcag ctcagaggag
     7381 atgaacagca ccgagagctg cacggcgagg ccgacgacga tgctgcccac gagggcgccg
     7441 taggcggtgc cgaggccgcc gaggaccacg ccggcgaaca tgagcagcag gagctggaag
     7501 ccaccgagcc agctgacctg ctcggtgagg ccgaggaaga tgccaccgac cgccgcgagc
     7561 gcggtgcccg cagcccagac cacgaggatg accttctcca cgtcgatgcc ggaggactcg
     7621 gcgaggtcct tgttgtcggc cacggcccgg gtggccttgc cgatccgggt gcgctcgagg
     7681 gcgagggcca cgaggaccag ccggatgatc gacaccgcga cgatccagat gtccttgcgc
     7741 accatcgtga tgttgaggaa gtcgatggtg tcggtctgga tcgcgtagtc gtcgtaggga
     7801 cgggtgcgac cgccgaactg gtagaggaac atgtgccgaa gggccagcga ggcaccgatc
     7861 gacacgacca tcagggcgat gaggctggtg ccgcggttgc gcagggggcg ccagaagccg
     7921 cggtcgaaca gcgcgccgaa ggcgccggac agcacgacgg cgatgggcgc ggcgatcagc
     7981 aggtggatgc cgaaggtgac gttgaagagg aaggcgagca gggcgccgaa ggtgaccagc
     8041 tcgccgtggg cgaagttcac gaggccggtg gtgccgaaga tcaggctgag cccgacggcg
     8101 cacatggcga tgatgaggcc gaacttgaag ccctcgaagg ccagctgcgg gacgcggtcg
     8161 agccggctcg gcccggtgcg ctcggagcca ccgccctcgc cctcgaccag cgggaacagg
     8221 acgttgcgct gctgtccggg gctcagctcg aacgtggtgg agccccgctc ctcgctgcgc
     8281 agcgacacgc cctcggggat ggtctccggg tccaccgaga tcgtgtactc accgggttcc
     8341 ggcagctcga ggaggaacgt gccgtcctcg gcggtggtgt cggaggcgac ctcctcgccg
     8401 tcggcgttgg tggcggagat ctccaccccc tcgaccggga cctcgttgtc ctccgcgtcg
     8461 tcgtacttga gcgacccgat caccgactcg atgtcctggt cagcggcctc ctgagcctcc
     8521 gggttggcct ccggatcgcc aggctcctcc gtcgcttcct gcgcgctggc gacggcggcc
     8581 cccacgaagg agaagccgat caccgacgtg agggcgacga gcaggagcac cagcctgcgg
     8641 gtgatggcgg acacgatcgg gggatcatag gcaaacgaac cggtgacacc agacacccag
     8701 gcgattcgcg cagttctgct cacccgcagc agcccggcga cgggtagctg accgagggcg
     8761 cgaccacccc gtcgacgtga ccgatctccg ccagcagcag cccgaccgtg tcgacgccct
     8821 cgaccgcggg cagatcgagg tggaagacgt cctcggccac cccggctcgc cgggacgaga
     8881 cccgcacatc ggtgatgtcg gcgccgagcg cgccgatccg cgaggcgacc gatcccagcg
     8941 cccccggccg gtccggcagc tggagggtga cgaccaggtg gcgccggccg gtcgccgtcg
     9001 tcgggtcgtg gtcggtctcg cccatcagcg ctcggtccat gcgtgcaggg aacccggcgc
     9061 gcgttcccgg ccgaccaccc gcgggtgaac gcttcgtgaa cggtgcggct tcagcgcaga
     9121 cccaccgcgt cgaggacctc gggcaggctg ccggtcatcc cggtgatgaa cgtcccgaac
     9181 tcggcgtaga tcgccgatgc ccggtcgtag cgcatcgtgt agacgacctc cttgaggtcg
     9241 tcgggcttct gggcgaacag ggtgacgccc cactcgaagt cgtcgacgcc ggtggagccg
     9301 gtgaccacct ggagcacccg gccggcgaag gtccgcccgg acttgccgtg ctccatcatc
     9361 agctccatgc gctcctcgta gggcagcgtg taccagttgc cctcgacggt gcgccgcttg
     9421 gacatcgggt agaagcagaa cgccggcttg ccctccggcg gcagctgcgg gaagaggcgg
     9481 gcctgcttca tctggtccgg cacgccctgg gcgtactcgc tgatctcggt gaggctcacg
     9541 tagctgtcga ccagctcgag gccggcggac accagctcgg tctggagcag gcggaggcgc
     9601 cactggtcgg ccccaagcgc catgaagccg atgtcggcct tgtgcccgag caccgcgacg
     9661 gggaccacct ggtagccgtc ggcctcggcg gacgccaccg cctcggacac cgcatcggcg
     9721 tcggcgaggg gcgtcagggc gaagaacagg tggagcacgg cctgcccgtc ggcggggctg
     9781 gagggggcgg gcgttgcggg ggcgtcgctc atgggccaca gcctacggag cacccccggc
     9841 cggtcggtca gtcgtacgcg cgcccgccgg cgctggtcgc ccagccgatg agcgtgatgc
     9901 gagccatccg ggcggcctcc acggcgaggg aggtcggggc ccccttggcc accacggtgg
     9961 tgatgccggc cgcccaggcc ttctgcacga tctcgaagcc gacccggccg ctgacggcga
    10021 ggccgaaccc gtgccgcggc agctcgccgg ccagcagctg gtggcccacc accttgtcca
    10081 cggcgttgtg gcgacccacg tcctcccgca ccacgaccgc ctccccggtg gggctgaaca
    10141 gcgccgcggc gtggaccgca ccggtggcgg cgaagatcgg ggtgtccccc agcagctccg
    10201 atggtgcggc gagcaggacg tcgtcgggga tgggctcgcc caccgggagc gggtggatgc
    10261 gggtccgaag ctcgtccact gcggtggtgc cgcacatccc gcatgcgctg ctggtgacgc
    10321 cgagccgggg ctcgggcacc ggcgccagcc caccggtgct gacggtgacg acgttgaact
    10381 ccgactcgac cacggcaccc gggccctcgc cgcggcagta cttggccgag cggaccgggg
    10441 catcgccgag caggccctcg gtgtggcaga agcccacggc cagctcgaag tcgtggccgg
    10501 gcgtgcgcat cgtggtgccc acgaggtgac cgtcgaggcg gatctcgagg ggctcctcca
    10561 ccgccacctc gtccggactc cgggagcggc cgtccgcgtc cacccacgtg gtgagcaggc
    10621 ggttggtgcg tccccgggtc acggacccag cgtaggagcg actcgcccgc gtccggcggg
    10681 ggcaggagga ggcgggtcgc gggacgaaca acaccggcgg ggaacacgac gaccatgacc
    10741 gacgaaccgc ttcgagccgc gcgcccgctc caccgaccgc accgacggtc ccgcaccgcc
    10801 ctggcggcgg ccctgctcgc gctccttgcc agcatcgtcg ccgtcccgac ggccatggcc
    10861 gccccctcgg cacccgccga aggcgtcgcc gtgcttcgcc tggacggccg tggccacggc
    10921 cacggcgtgg gcctgtcgca gtggggcgcc cgatcgatgg cggcccaggg cgccgacgcc
    10981 gccaccatcc tcgcccacta ctacccgggc accgagaccg gctcggccgg cggcgaggtg
    11041 gtcgtcggca tcgccagcgg cgcccgcagc acgatctcgc tcccccaggg cggggagctg
    11101 cgctccgcgc gcggcggcgc ccaggccgag ggcttcccga tcccgctcgg tcccggtgag
    11161 gtggtcacca tccaccacga cggcagcggc tggcgcgtgg agcgcggcgg cgtgcggggc
    11221 ctcgacgccg gcaacgccca gatgttccag gaggccgacg actgcgtgct gctctgcccg
    11281 ccggaggaca ccccggaccc gcagcccgca ccggagcccg gcaccgaacc ggaaccggaa
    11341 ccccagccgg agcccgagcc cgacgactgc gccctgtgcg ggccccccga ggagcagcct
    11401 gacgcgcagc ccgcgcccca gccggcgccc ggcccgcagc cgggcgcaga ccagcaggcg
    11461 cctcgctcgc cgtccccgat ctgggcggtg cccgtcgacg gcggcacggt ccactccgtc
    11521 gaccgggggc gcacctaccg gggtctgctc gaggtgggcg gcggtgccgg ttccgtgcgg
    11581 gcccgcaacc acgtcgacat cgaggactac ctccgcggca tggccgaggt ccccggcacc
    11641 tggcctgccg cggccgtgca ggcccagacc gtcgcagccc ggacctacgc cctgcgggcg
    11701 atggccggcg ccggggagat ctgcgactcg gagtcatgcc aggtgtacgc cggcgtggcc
    11761 cgcgagagcg ccgggcagga cgctgccgtg gccgccaccc gcggctgggt cctcacccac
    11821 ggcggggccc tggcggccac cttctactcg gcttcggccg gcggccactc cgccaccgtc
    11881 caggaggggt tcggctcgtc ctacgacatc ccatacctgc ccgcccgtcc cgagcccacc
    11941 gacgacaccc gcccctggaa cctcgaggtc tccctcggcg acgtcgccgc ccgcctcggc
    12001 tacccgggtc gcctcaccga cgtgcgcgtc gacgccgccg gcccctccgg ccgcccgctc
    12061 gccatgaccc tggtcggcga cgccggtgaa cggccggtgg acccgcaggt cttccgccgc
    12121 cgcctcggac tccgctccac cttcttcacc gtccagtccg gcgtcgccgg gcaggccccg
    12181 ccgcccccgc cggcgcccgt cgagagcgac cagctcctca ccgccgatgc cgtcgtctcc
    12241 gacgacgtcg ggccggtccg gccggcccgg gcccgcagca tggtcgtggc cagcccccgc
    12301 tcgctgaccc cggccgacgg gctggcggcg gcacggaccg ggcaggtcgc ggtcctcgcc
    12361 ctgctcgccg cggccgccac gtggttcgcc gtcacccggc tccacgccgt gggccggacg
    12421 acgtcctcca gggggctcgg caccgcgccg ctcggcgccg cgccgatcgg gtggcgggag
    12481 cagctcgctg cgacaatgac cccgtggagg aaccacaacc cctgacggcc acgcgggtcg
    12541 tgctgcgcgg gctgctgcgc cgctgcccgc tgtgcgggag cacggggatc ttcgagagct
    12601 ggttccgcca ggtcgagcgc tgcccccggt gcaactaccc gacgacccgg gtcgaggacc
    12661 agtggatcgg ctcgctcggg atgaacatca tcgtgagctt cacgctgctc ctcggggtca
    12721 tcgccgtcgg cttcgcggtg acctacccgg accctcccgt cggggtgctg ctcgtgctgg
    12781 cggtcagcgt ggccgccctg ttccccgtgt tcttcttccc gatctcccgc tcgctgtggt
    12841 cggccatcga cctggcgatg cgcccacccg accccgacga cgacgtcgac ccccgcttcc
    12901 tgccgccgac ccgccacggt ccgggctgac ctccagacgt tcccttcgcg tgatccgacc
    12961 gctcagcgtc ggatcgcgcg aacagaagca ggagggtcag gcggtgggga cgtgggagtg
    13021 gtcgttgtgg cggacgagcg accaggtccg ctccttgacc cacggcatct ccgggcgctc
    13081 ctggtgctgc cactcggtga cgccgcagtt ctggatgagc accgatccga gccagccggt
    13141 catcccccgc ccgtggaacg cggtgaagct gtgctccacc acgccgccgt ggcacgcaac
    13201 cacgacggtc tcgccccgat gggtgcgagc gatgtcgtcg agcgcgcccc cgacccgggc
    13261 cgcgaagccg gcccaggtct ccgctccagg gacgtggtgg tggaacgggg tccagacgtc
    13321 gggggtcggg aagcgctgct cgagctcctc ccacgccatg ccgtcgccct cgggcccgat
    13381 gtcgaactcg cgcaggtcgt cgcgctcgac gacgtcgagg ccgccgatgg caggggcgat
    13441 gatcgaagcc gtctccgacg cccggggcag ggtcgaggcg tagagggcgt ggacctcgcc
    13501 gagctcggcg gtgcggtcca ggcgatcccg cagggccgtg gcctggcgtc ggccgagctc
    13561 ggacaacccg gtgcagcccc ggcggccacc caccactccc tcgacgtggg actgggcctc
    13621 gccgtggcgg acgaggacga tgcgcgtggt cgtcatgagg tgaccgtacg cgggacgggc
    13681 gccccgcagg gcgcccgttc cggtgttcgt ggttgcggcc ggagccgtgg acgactccta
    13741 gaagtcgtcc atgccgccgg gcatgccgcc gccggcgccg tccttctcgg gcttgtcggc
    13801 gatgacggcc tcggtggtga ggaacagcgc cgcgatcgag gctgcgttct gcagcgccga
    13861 gcgggtcacc ttggcggcgt cgatgatgcc ggccttggtg aggtcctcgt agttgccggt
    13921 ggccgcgttg aggccctcgg tcgaacccga catggccagc acccggtcga ccacgacgcc
    13981 gccctcgagg ccggcgttgc gggcgatctg cttgaccggc tcctgcaggg cgtgggccac
    14041 gatcttggcg cccgtggcct cgtcaccgac gaggtcgccg acgagctggt cgaccttggc
    14101 cttggcccgc agcagggtgg tgccgccgcc ggcgaccacg ccctcctcga tggcggcctt
    14161 ggtggtgctc accgcgtcct cgatgcggtg cttcttctcc ttgagctcca cctcggtggc
    14221 tgcgccgacc ttcaggacgg cgacgccgcc ggacagcttg gcgaggcgct cctggagctt
    14281 ctcgcggtcg tagtcggagt cggtgttgtc gatctcggcc ttgatctggc cgatgcgacc
    14341 ctgcaggtcg tcgtcggagc cgccaccgtc gacgatggtg gtctcgtcct tggtgacgac
    14401 gatcttggcg gcggtgccga gcaggtcgag ggtggtgttc tcgagcttga ggccgatctc
    14461 ctcggagatg acctggccac cggtgaggat ggccatgtcc tgcagcagcg ccttgcggcg
    14521 ctcgccgaag ccgggggcct tcacggccac ggccttgaag gtgccacgga tcttgttgac
    14581 cacgagggtg gcgagggcct cgccctcgat gtcctcgccg atgatggcga gcggcttgcc
    14641 ggactgcatg accttctcga gcaccggcag caggtcgcgc acggcggtga tcttggagga
    14701 gacgaggagg atgtaggcgt cgtcgagcac ggcctccatg cggtcctggt cggtgacgaa
    14761 gtacggggag atgtagccct tgtcgaagcg catgccctcg acgaggtcca tgtccatgcc
    14821 gaaggtctgg ctctcctcga cggtgatgac cccgtccttg cccaccttgt cgatggcctc
    14881 ggagatcatc gtgccgatct cttcgtcagc ggaggagatg gaggcgacct gggcgatctg
    14941 gcccttgctg tcgacctcga cggcctggtc cttgatggac gccaccgcag cctcgacggc
    15001 ctgctcgatg cccttcttga gcgacatgga gttggcaccg gcggccacgt tgcgcaggcc
    15061 ctcgcggacc atcgcccacg ccagcaccgt ggcggtggtg gtgccgtcac cggcgacggc
    15121 gtcggtcttc ttggcgacct ccatgaccag ctcggcgccg atgcgctcgt acgggtcctc
    15181 gaggtcgatc tccttggcga gggagacgcc gtcgttggtg atggtggggg cgccccactt
    15241 cttgtccagc acgacgttgc ggcccttggg gccgagcgtc accttgaccg cgtcggccag
    15301 cgtgttcatg ccacgctcga gggcgcggcg cgcttcctca ttgaactcaa tcagctttgc
    15361 cagggtgttt cgggagtcct cccgtcaagt ggggccggag ccccggagtg tgcagtgtcg
    15421 ggtgaacc
//
LOCUS       DATISU010000022         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_746149, whole genome shotgun sequence.
ACCESSION   DATISU010000022 DATISU010000000
VERSION     DATISU010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746149"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VLR27_01120"
     CDS             <1..321
                     /locus_tag="VLR27_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HSP02069.1"
                     /translation="DPDWYIHRHRTEDEVLPWDHLSAGLHKDFLWMDWQDALAEVGLP
                     DCRWTPCYDCGACTGYSIEHIVASATPPAGGSQGTGVDLSAGHSVPVELTPRIPSGAP
                     GSQR"
     gene            318..1115
                     /locus_tag="VLR27_01125"
     CDS             318..1115
                     /locus_tag="VLR27_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HSP02070.1"
                     /translation="MTPPRTERIRIRFDKFGRIRFTSHRDVARVWERSLRRSRLPVSY
                     SEGFSPRPRLRFGLALATAHESCGEYLDVDLEPGAVSSGRVDIDELVPLVNPMLPPGM
                     TVQATAHVEPGAPSLQESVDSCTWLVDVPQMTPAQAHDLVERALAAEELPVTRTRKGK
                     SVTDDIRPNVLALRVADPDERPPAADWQPAGALLRAELGTHPRACRPAELLAAVDPTL
                     EEGRVVRLHQWTMVDGVRREPVPLEATPPTDAGIARSSTAHAIERAS"
     gene            1131..>2000
                     /locus_tag="VLR27_01130"
     CDS             1131..>2000
                     /locus_tag="VLR27_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HSP02071.1"
                     /translation="MSDTQHGSSTSNDQRPDGAGKAADAARDGDDTSKPKIGDTRPSP
                     RADDGGSDGGGDDGGAKPGRRRRRRGGRNRGKGRADDQQRPVELDTGFDAVELDEAQL
                     EARRGKHRNGKPVGRYLMAVHRTEQATQIAVLEGRQLIEHYVAKPADDISQIHGNIYL
                     ARVANVLPGMEAAFVDIGTPKNAVLYRGDVAYDPDDVEGGGKPKIEDVLKSKQTIVCQ
                     VTKNPIGVKGARLTQEVSLPGRFVVLVPNSSTYGISKRLPDDERKRLRKILDGVKPDQ
                     HGVIVRTAAENVTA"
BASE COUNT          321 a          801 c          653 g          225 t
ORIGIN      
        1 gaccccgact ggtacatcca ccggcaccgc accgaggacg aggtcctgcc ctgggaccac
       61 ctctccgccg gcctgcacaa ggacttcctc tggatggact ggcaggacgc gctggccgag
      121 gtcggcctgc ccgactgccg ctggaccccc tgctacgact gcggtgcctg caccggctac
      181 tcgatcgagc acatcgtcgc ctcggccacc ccgcccgccg gcgggagcca gggcacgggc
      241 gtcgacctga gcgccgggca cagcgtcccg gtcgagctca cgccccgcat cccgagcggc
      301 gcgcccggga gccagcgatg acaccgccga ggaccgagcg gatccgcatc cgcttcgaca
      361 agttcgggcg catccgcttc acctcccacc gagacgtggc ccgcgtgtgg gagcgcagcc
      421 tccgccggag ccgactgccg gtgtcctaca gcgagggctt ctcgccccgc ccccggctcc
      481 gcttcggcct cgccctggcc accgcccacg agtcctgtgg cgagtacctc gacgtcgacc
      541 tcgagcccgg cgcggtgtcg tcgggccgcg tcgacatcga cgagctcgtc ccgctcgtga
      601 acccgatgct gccgcccggc atgaccgtcc aggcgaccgc acacgtcgag cccggcgcgc
      661 cgagcctgca ggagtcggtc gactcctgca cctggctcgt cgacgtgccg cagatgaccc
      721 cggcgcaggc gcacgacctg gtcgagcgcg ccctcgccgc agaagaactc ccggtcaccc
      781 gaacgcgcaa gggcaagtcg gtcaccgacg acatccgccc gaacgtgctc gccctccgag
      841 tcgccgaccc cgacgagcgc ccgcctgctg ccgactggca gccggccggc gccctgctcc
      901 gagccgagct cggcacgcac ccgcgtgcct gccggcccgc cgagctgctc gccgcggtcg
      961 acccgacgct cgaggagggt cgggtggtcc ggctccacca atggacgatg gtcgacggcg
     1021 tccggcgcga acccgtcccg ctggaggcaa cgcctccgac ggacgcaggg atcgcccggt
     1081 cgtcgaccgc gcacgccatc gagcgtgcgt catgagaagg gacccccacc atgtccgaca
     1141 cccaacacgg gtcgtcgacg tcgaacgacc agcgccccga cggcgccggg aaggccgctg
     1201 acgccgcccg ggacggcgac gacacctcca agcccaagat cggcgacacc cgcccctcgc
     1261 cccgcgccga cgatggcggc agcgacggcg gtggtgacga cggcggcgcc aagcccggcc
     1321 gtcgccgccg gcgccgcggc ggtcgcaacc gcggcaaggg ccgtgccgac gaccagcagc
     1381 gcccggtcga gctcgacacc ggcttcgacg ccgtggagct cgacgaggcc cagctcgaag
     1441 cccgccgcgg caagcaccgc aacggcaagc cggtcggccg ctacctcatg gccgtgcacc
     1501 gcaccgagca ggccacccag atcgccgtgc tcgagggccg ccagctcatc gagcactacg
     1561 tcgccaagcc cgccgacgac atctcccaga tccacggcaa catctacctg gcccgggtgg
     1621 ccaacgtgct gccgggcatg gaggccgcgt tcgtcgacat cggcacgccg aagaacgcgg
     1681 tgctgtaccg cggcgacgtg gcctacgacc ccgacgacgt cgagggcggc ggcaagccca
     1741 agatcgaaga cgtcctgaag tccaagcaga ccatcgtctg ccaggtcacc aagaacccga
     1801 tcggggtcaa gggtgcccgc ctcacccagg aggtcagcct cccgggtcgc ttcgtcgtgc
     1861 tggtccccaa ctccagcacc tacggcatct ccaagcggct gcccgacgac gagcgcaagc
     1921 gcctccgcaa gatcctggac ggcgtcaagc cggaccagca cggcgtgatc gtgcgcacgg
     1981 cggccgagaa cgtgacggcc
//
LOCUS       DATISU010000023         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_47619, whole genome shotgun sequence.
ACCESSION   DATISU010000023 DATISU010000000
VERSION     DATISU010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47619"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VLR27_01135"
     CDS             <1..970
                     /locus_tag="VLR27_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HSP02072.1"
                     /translation="EVRKFANGALARTEFSSATGGWSAGGTFPVVEDAGDAVLSNPNH
                     SWKTTLTRERIETKYPSIGTLTDIQILDRNGIGDMGGRVLELRVVGTDGDVERTGNQF
                     RSDFVLKSDWFAITVPPPPPVTARPITTACPEGEVPRDVFDDVDPDSVHALAVDCVAW
                     REIAEGTSATTFSPNTSVTRGQMASFVARFVIAAGGSLPEDPADAFDDDEGSVHEDAI
                     NQLAAVGIVRGTGVRTYEPSTRIDRGQMASLIARALDHLDVPLEESPPSPFRDDNDSV
                     HELAIDQLAAEGVVTGTAPGTYSPKDTARRDQMASTLARALDLALS"
     gene            complement(987..>2465)
                     /locus_tag="VLR27_01140"
     CDS             complement(987..>2465)
                     /locus_tag="VLR27_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSP02073.1"
                     /translation="VIDTGVAQPSATGGSGPPDLAGTSFVPGWDFVEGDDTPQDENGH
                     GTHVASTIAATTGNGIGVAGVAPGTTIMPLRVLDEDGRGEDWHVAAAVRWAVDNGADV
                     ANLSIGSTTASQVLLDAIDYAHRNGVTVVAASGNEAQLTGKVLWPARVPSVVAVGAVL
                     RDGVRASYSNFGPELDIVAPGGDLRIDEDGDGRPDGILQESLDPADRTRYCYCYLAGT
                     SMAAPQVSAVAAMLISLGVSEPAEVKRILEASTWDLGPVGRDDEYGNGLLDARAAVLH
                     AIGEPPPAAGTVRGIRQACPPDQVPPGRFDDVGDTVHAAAIDCVAWWGVTSGATATSY
                     EPRGTVSRAQMASFLVRLIADAAGVPLPASAPDAFTDDEDSVHEDAIDQLHALGVIRG
                     RADGTYGPGDPVTRAEMATFLARTHDLIADPALRPGADRFADDDGSVHERSIGAVAAV
                     GVAAGTSETRFQPNVPVRREQMATFLVRLLDLLVDAGDVAEP"
BASE COUNT          328 a          932 c          880 g          325 t
ORIGIN      
        1 cgaggtgcgg aagttcgcca acggcgccct ggcccgcacc gagttctcct ccgccaccgg
       61 tggctggtcg gccggcggca ccttcccggt cgtcgaggac gccggcgacg ccgtgctgag
      121 caacccgaac cacagctgga agaccacgct cacgcgggag cggatcgaga cgaagtaccc
      181 cagcatcggc acgctcaccg acatccagat cctggatcgc aacggcatcg gcgacatggg
      241 cggccgcgtg ctcgagctgc gcgtggtcgg cacggacggc gacgtcgagc gcaccggcaa
      301 ccagttccgc agcgacttcg tgctcaagtc cgactggttc gccatcaccg tgccgccacc
      361 gccaccggtg accgcccggc cgatcaccac ggcgtgcccg gagggagagg tcccccgcga
      421 cgtcttcgac gacgtcgacc cggacagcgt ccacgccctc gccgtcgact gcgtcgcctg
      481 gcgcgagatc gccgagggca ccagcgccac caccttctcc cccaacacct cggtgacccg
      541 ggggcagatg gcctccttcg tcgcccgctt cgtcatcgcc gccggcgggt cgctgccgga
      601 ggacccggcg gacgcgttcg acgacgatga gggctcggtc cacgaggacg ccatcaacca
      661 gctggcggca gtcggcatcg tgcgcggcac cggcgtgcgc acgtacgagc cgagcacacg
      721 catcgatcgc gggcagatgg catcgctcat cgcccgggcg ctcgaccacc tcgacgtccc
      781 gctcgaggag tcgccgccct cgccgttccg tgacgacaac gactccgtgc acgagctcgc
      841 catcgaccag ctggctgccg agggcgtggt gaccggcacc gccccgggca cctactcccc
      901 caaggacacc gctcggcgcg accagatggc cagcaccctc gcccgggccc tcgacctggc
      961 cctgagctga tccggtccgg gcccgctcag ggctcggcga cgtcgccggc gtcgaccagc
     1021 aggtcgagca ggcggaccag gaaggtggcc atctgctcgc gccgcaccgg cacgttcggc
     1081 tggaacctcg tctccgacgt gccggccgcg acgccgacgg ccgccaccgc accgatgctg
     1141 cgctcgtgga cggagccgtc gtcatcggcg aagcggtctg cgcccggccg gagggccggg
     1201 tcggcgatca ggtcgtgggt gcgagcgagg aacgtggcca tctcggcacg ggtgaccggg
     1261 tcacccggcc cgtaggtgcc gtcggcacgg ccacggatga cgccgagcgc gtgcagctgg
     1321 tcgatggcgt cctcgtgcac cgagtcctcg tcgtcggtga aggcatcggg cgccgacgcg
     1381 ggcagcggga ccccggcggc gtcggcgatg aggcggacca ggaacgacgc catctgggcc
     1441 cgactcaccg tgccgcgggg ttcgtagctc gtcgccgtcg cgcccgacgt gacgccccac
     1501 cacgccacgc agtcgatggc ggcggcgtgc acggtgtcgc ccacgtcgtc gaaccgcccc
     1561 ggcggcacct ggtccggcgg gcaggcctgg cggatcccgc gcaccgtccc cgccgccggt
     1621 ggcggctccc cgattgcgtg gaggacggcg gcccgggcgt cgagcaggcc gttgccgtac
     1681 tcgtcgtccc ggccgaccgg cccgaggtcc cacgtcgatg cctcgaggat gcgcttcacc
     1741 tcggcgggtt cggacacgcc gaggctgatc agcatcgccg ccaccgcgct gacctggggg
     1801 gccgccatcg acgtgccggc gaggtagcag tagcagtacc gggtccggtc ggcggggtcg
     1861 aggctctcct ggaggatgcc gtcgggccgt ccgtcgccgt cctcgtcgat gcgcaggtcc
     1921 ccgcccggcg cgacgatgtc cagctccggg ccgaagttgc tgtacgacgc ccgcaccccg
     1981 tcgcggagga ccgctcccac ggcgaccacg ctgggcaccc gggccggcca caggaccttg
     2041 ccggtgagct gggcctcgtt gccgctcgcg gccacgaccg tcacgccgtt gcggtgggcg
     2101 tagtcgatgg catcgagcag gacctgcgac gccgtggtgg agccgatgct caggttggcc
     2161 acatccgcgc cgttgtcgac ggcccaccgg acggcggcgg cgacgtgcca gtcctcgccc
     2221 cggccgtcct cgtcgaggac ccgcagcggc atgatcgtgg tcccgggggc caccccggcc
     2281 acgccgatcc cgttgccggt ggtggcggcg atggtgctcg ccacgtgggt gccgtggccg
     2341 ttctcgtcct ggggggtgtc gtcaccctcc acgaagtccc agcccgggac gaagctggtg
     2401 ccggccaggt ccggcggccc ggagcccccg gtggcgctgg gctgggcgac gccggtgtcg
     2461 atgac
//
LOCUS       DATISU010000024        15128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_792788, whole genome shotgun sequence.
ACCESSION   DATISU010000024 DATISU010000000
VERSION     DATISU010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 15128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15128
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792788"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VLR27_01145"
     CDS             <1..262
                     /locus_tag="VLR27_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02074.1"
                     /translation="GCRAVNVGTGTGSSVLEVLAAAERAVGHEIPHEVVDRRAGDAPC
                     VFADTTLATELLGWSPSRTLDDMCADHWRWQRENPEGFGSTR"
     gene            259..720
                     /locus_tag="VLR27_01150"
     CDS             259..720
                     /locus_tag="VLR27_01150"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSP02075.1"
                     /translation="MRGSGDGFVQCSDGHVRWGRYGAAGVLFVHREADGPRVLLQKRS
                     QWAHEGGTWSVSGGALDQGETPEEAALREASEEVGDIPAHRIVGEYVFAPADDWSYTT
                     VVVEVDGRFGFSANFETDAVGWFRLDEVDDLPVHAGFAAAWPALRRIVEGG"
     gene            817..1371
                     /gene="infC"
                     /locus_tag="VLR27_01155"
     CDS             817..1371
                     /gene="infC"
                     /locus_tag="VLR27_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HSP02076.1"
                     /translation="MARRTPFPRRSESKHRINGEIRAERVRLIDETGEQVGEMPLPAA
                     LEASRRAGMDLVEVAPDARPPVARIMDHGKYAYEQSVKARAKKKRSGGGVKEMKYRPN
                     IGDHDFLTKTRKVADFLTKGNKVKLTVWLRGRERSMPGLAEDVVERVVTEVDDMVDDD
                     ITVEAPVRGDGPQRSAVIATSAGD"
     gene            complement(1378..3093)
                     /locus_tag="VLR27_01160"
     CDS             complement(1378..3093)
                     /locus_tag="VLR27_01160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HSP02077.1"
                     /translation="MDGVDTVPPMDARQRAQDLARMRDEVFDIVVIGGGVTGAGVALD
                     AVTRGLSVALVEQRDFAAGTSSRSSKLFHGGLRYLEQLELGLVTEALHERNLMLNRLC
                     PHLARPVTFLYPLTHRVWERAYVGAGIALYDVLAAFGSNPLPRHRHLSKRAARRLVPS
                     LRDDVLTGAITYSDAQVDDARHTMSLVRTAHHHGAAVAASVQVVDLLRDGGRVTGVRA
                     RDVESGDELDIRARQTVNATGVWTDEIQDMAGGAALRVRASKGVHLVVPRDRIDAGAG
                     LICRTPTSVLFVIPWGDFWLVGTTDSDWNLDLARPAASAADLDYLLGQANRWLRPALT
                     HGDIVGVYAGLRPLLHGESDSTSKLSREHAVTAVTPGLLSVAGGKYTTYRVMAADAVD
                     AAVAAMGVDAPASCTETVPLVGATGYQAMRNRRTQLAEESGLPVDRIDRLLGRYGSEL
                     PVLLELLRADPAMAECLPGTDDYLAVEARFAVAQEGALHLDDVLTRRTRLSIESPDRG
                     VSAAPVVAGIMADVLGWDEATVAREVDYYSKRVEAERESQQAVDDHTADARRLGAEDI
                     RGNLT"
     gene            3183..4877
                     /locus_tag="VLR27_01165"
     CDS             3183..4877
                     /locus_tag="VLR27_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02078.1"
                     /translation="MTSGGQSGPSRRAPRRRTLLRTAVAGATIGVTLAGAALAGVVAA
                     PVDDPVGDAVGGIAGPVEGVLDGSGGGDDGAMVEELAAVEAAPTSSGLRAGAAKHSIA
                     PRPEDFGGTWVTDPAACRTLDPGFLERLGTTPEDAAHLAHTGTPWPENPDCIYMGGFG
                     LGPMNPVSSFDTELGLWVRSIAVGDGADTVVLTIVDGEGWLWDYANKCDDCGAKQLSE
                     DLGAELGIDPAGIVIAATHSHAAPDFLGGWGFVPDWYMTQVTETIRRSVRDAVAAMEP
                     AVVEFGEVQARGFNRERRDTYRSAEEQQVGWVRAYVPGTPSKNPNKPGEPPRVIATLG
                     AYAAHPTTRGTNDGVAHPDWPGEFERAVEDRFGGIGLHFMTGLGNMSAAGETGPGIAA
                     LVPPVGQGQAVAVPSVSASQARWQQPVTNVPLSALGLPGFFDRRFEPVPASVSVGKGD
                     TAPCTSASAVSVELAASAIRIGDRLAITTGPGELFSNLTNTIKERSPAAVTLPLAQAN
                     DALGYMPQSFELDPVGQQGLGFAFSGYLFVNYEDSYAVDRCVGDMVLETTLDLLGG"
     gene            complement(4874..5644)
                     /locus_tag="VLR27_01170"
     CDS             complement(4874..5644)
                     /locus_tag="VLR27_01170"
                     /inference="COORDINATES: protein motif:HMM:NF015820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HSP02079.1"
                     /translation="MLVLVPPSEGKAVGSRGAPLDLDGLSFPELTATRRRVLDALVSA
                     SGRPDALAVLDAPKGAADRVAANTDLWSAPTLRVADLYTGVLYDALDLGSLDTAAKRR
                     ATRRLVVVSALFGALHPNDRVPSYRLSMGVDLPGVGPLARSWREPLGEVLPAAAGRGV
                     VVDLRSSTYAAAWKPAGELAERTVAVHVVRDGPGGRTAVSHMAKHTRGLVARELLVSG
                     ADPRTPDELVESLSGSFSVDIDEARPGRARRLEVVEPG"
     gene            complement(5644..6567)
                     /locus_tag="VLR27_01175"
     CDS             complement(5644..6567)
                     /locus_tag="VLR27_01175"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HSP02080.1"
                     /translation="MTVPPMTTADVDALRTRLSNWDRWGADDQLGALNHIRAEHRAAA
                     AATVRSGRTVSCARPLPTLPAPENPSPAEHHMIGTHTEGYGADWIGVASHGYATSHLD
                     ALCHIFWDGRTYNGHPTEVVTAHGAEQLGVHHLRDGVVGRGVLLDIPLVRGVDALEPG
                     TPITVEDLEAAESRAGLTVGTGDILLVRTGRWVWHREHGGWNPRDQLAGLHASCLPWL
                     HEREVATLGSDGVSDVHPSHVEPTDGVPTRLPVHTVGIVAMGLHLLDNLDLDDLAAAC
                     AEEDRWAFLLTVAPLIVVGGTASPVNPIAVL"
     gene            complement(6564..7208)
                     /locus_tag="VLR27_01180"
     CDS             complement(6564..7208)
                     /locus_tag="VLR27_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HSP02081.1"
                     /translation="MSGTADPEEPGDVGVGPHPEPWPDDPRLDPELLAGGDRRNVVDR
                     YRYWRLDAIVADLDQRRHPFHVAIENLQHDLNIGTVVRNANAFLAEAVHIVGRRRWNR
                     RGAMVTDRYQHVHHHPDVDALGAWAAAHDLPVVGIDNLPGSVPLEQVALPRRCVLLFG
                     QEGPGLSEAARDRLDLVCSIAMFGSTRSINAGVASGIAMHAWIRQHALGEDARS"
     gene            complement(7205..7798)
                     /locus_tag="VLR27_01185"
     CDS             complement(7205..7798)
                     /locus_tag="VLR27_01185"
                     /inference="COORDINATES: protein motif:HMM:NF039596.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HSP02082.1"
                     /translation="MSSRLPAARRREQLLEVALRVFASRGFHQTSMNDVADAAGVTKP
                     VLYQHFTSKRALYLELLELVSLRLMAAIDEATSAAAGPREQVERGLRAYFRFVVDSPD
                     EYQLMFGGGTRRDAEFAQEAARLERSIAEVIADLITVEGLAEHERVLLAHGIVGLAEG
                     ATRHWLGDDLDLDADEVTDRVADLAWRGLRGIRSEPR"
     gene            7914..8078
                     /locus_tag="VLR27_01190"
     CDS             7914..8078
                     /locus_tag="VLR27_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02083.1"
                     /translation="MPSRNSAPAATTRRPAQPIRPVPATRLAADLLAVKARRRRFQQI
                     LPPRAGERGW"
     gene            complement(8092..8388)
                     /locus_tag="VLR27_01195"
     CDS             complement(8092..8388)
                     /locus_tag="VLR27_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02084.1"
                     /translation="MPDPARPDRSDPVLERRRRIAGLVRTGQRVGYGLFGVAIALFAV
                     GLVVGFGGAITPGIVASIVIGSIVLAPAIVFGYGVKAAEREDRSQGRSTLGRPE"
     gene            8576..9976
                     /locus_tag="VLR27_01200"
     CDS             8576..9976
                     /locus_tag="VLR27_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02085.1"
                     /translation="MQMQLNAFKRTLAMGAVWTVAMGGLLATSPAASGQEGVEPGLGN
                     AYAQGIKVDPRSGRLSFGITYGMALAGHQNNVAVAESRSADLGVIGTTLGGEGCDGGD
                     PTLPKEDQPQPLVARSTEEGAGEEKVESENGVEKRALASGEPLARSVAVTAASGEDAV
                     LKIGATKSTTESGIIDGARVARAVTEVSGVSFAGGQVVLRGLRWEAAYQTAPTEATSG
                     SFTIDGIEVAGEKLPIPEENPFEPLAEANAVLEPLGFRITPPTVRNESGIQFVDPMRI
                     GIIPATARESVLGPVFNGIQPVRESVFDAIIEADCGNAAYITVLDLVLGSVSGAGSLG
                     LELGGVTATSNEIAFTSFLGSLGDAPSLPPLSSGAGSSPSLSGSGSSPSLSGGSSAPA
                     GAAATTPPTTVASAPADGGGGEQAAIADVEPLTGSRGGALAGVGLGGLALLAALAAAD
                     RAQMRRAQRSILMEVS"
     gene            9978..11699
                     /locus_tag="VLR27_01205"
     CDS             9978..11699
                     /locus_tag="VLR27_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSP02086.1"
                     /translation="MPAPTPQPTPQGSFPVNKRILRGYGPLAVFAAMLVLMAMIVPTT
                     VPNQQVSADGGTPGAGQGFSTSGSSSGSSGTSSAGTATTAPAGGADPAAAPAEGGGEG
                     VASDGEGSGIAPVEGVQACPDRQEQIPGDPYSPPCVAWAGGNNGGGTTKGVTEDTILV
                     SYRVLGGEKGFQQTLAELAGASLSDTPETIQNTITAFSEYFNQRFEFFGRQIKVQFYD
                     GQGSNTNELLGKGRDRAEADATKVAEEIGSFADLSATSEPYAGALASRGVIGFGTPYL
                     SREWHEQRAPFAWSLATDGTRVSELAAEYGNKRLHGKNADFAGGNLKGQPRKFATIAP
                     ENSWYQESVNNARRITEEAGNDPGMNVKYQLDLSTMSSQANNIIPKLKNSGVTTVVCG
                     CDPVLPVFLSGVANREQYFPEFIIAGTALTDADIVGQLWEPAFAKQAFGVSSLTDFVP
                     PTQTIAYEAFKTVRPNEEPAFSVDLIYYQMYMLAIGLQGAGPNLTPQSFSDGMFNYPA
                     RSGPVGLWKFGPGDRTAANDVREIYFDINAISPYNGKQGAYISTSAERWETGQIPEGN
                     PPIPGGA"
     gene            11699..13891
                     /locus_tag="VLR27_01210"
     CDS             11699..13891
                     /locus_tag="VLR27_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP02087.1"
                     /translation="MTAVLDRVPGAAALDEMSTKAKAGLAIAALVVGYALLYVLPGTG
                     DFLRDKAPYGVLFIGTVQGTTTALLAMGLILIYRTNRFVNFSYAAMGALPGAFALGLH
                     LEQGLPFFAALALAVVIGVGVGFLTELLIRRFKGASRLILTVASIGLAQIFGVLSLLA
                     NKAIGFISLAGGFTIPLDVRFTLDVKTMTGDEILIIAVVPAVIAGLAWFLLKTDAGVA
                     IRAAAENEDRALLLGIPVYKLGRIVWMIAGGLAALTYVLQAPSAGVSPSVVSAGPAVL
                     LPALAAAVVARLESLPMAFAAGIGLGMLDQLVRWNGTGSPSITTMFFLAVILIALLLQ
                     RASLSRAMNSESAAFSSEGVIKPVPLELRHLPEIRIAKVVIGVAVLALLVFVPLSWGP
                     SNQLLAAIAMVWAMVAVSLVILTGWGGHISLGQFAIAGMGGLLAGNVLQRGDIDYFVV
                     LTGAGLVGALTALIVGLPALRIRGLFLAVSTLAFAVALNSYVLNRDNFSSIIPGDVTR
                     PVLWERWDLERGYPMYAVCLSFLLLSVLAARSVRGTRAGRVIIATRDNERAADSASVP
                     TTRIKLAAFLVAGTIAGIAGALHVTILHGLNQGTYSPEMSLEVFSTAVIGGLSSVSGA
                     IGGVLLFQWLGSITALGDVRQLISGAGLLIVLLVFPGGLAQLACNVRDRFLRRIAERH
                     DILVPSLVADKRVEEEDKGEDESLLRGALGGDDEPTTTTHDELVGSAR"
     gene            13888..>15128
                     /locus_tag="VLR27_01215"
     CDS             13888..>15128
                     /locus_tag="VLR27_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSP02088.1"
                     /translation="MTSTNGRSVTTSRRRAPLRAEAGGVPDLGTDETLVLDTPVANGH
                     RPADAADRAAAPEVGTETILKCSGIDVSYGPVQILFGVDLEVKRGEVVALLGTNGAGK
                     STLLKAICGLKAPKAGTVEFKGEDITGLSADLVADKGAALMPGGKGIFPTLTVAENLR
                     LASWLIKDDPQRVKESKAEVLELFPILSERSNQLAGDLSGGEQQMLALGGALMTSPEL
                     LMIDELSLGLAPTIVARLLEVVDEIHRRGTTIIVVEQSVNVALNLAERAVFMEKGEFR
                     FTGPTRELLDRPDILRSVFIAGGKADEADDADKAETGAQPKRVSTVAEDARAVLQCRG
                     VTKRFGGITAVDSVDVDLHDGEIIGLIGHNGAGKTTLMDCISGFLAIEDGRIGIQGLD
                     VTDWQPHERARAGLGRSFQDAR"
BASE COUNT         2097 a         5641 c         5341 g         2049 t
ORIGIN      
        1 cggctgccgg gccgtgaacg tcggcaccgg caccggctcg tcggtgctcg aggtgctcgc
       61 tgcggccgaa cgggccgtcg gccacgagat cccccacgag gtggtggacc ggcgggcggg
      121 cgacgccccg tgcgtcttcg ccgacacgac cctggccacc gagctgctcg gctggtcgcc
      181 ctcgcgcacc ctcgacgaca tgtgcgccga ccactggcgc tggcagcggg agaaccccga
      241 gggcttcggg tcaacccgat gaggggcagc ggcgatggct tcgtccagtg cagcgacggc
      301 cacgtccgct ggggccggta cggggcggcg ggcgtgctct tcgtgcaccg ggaggccgac
      361 ggtccccgcg tgctgctgca gaagcggtcg cagtgggccc acgagggcgg gacgtggtcg
      421 gtgtccgggg gcgcgctcga ccagggggag accccggagg aggccgcgct gcgggaggcc
      481 agcgaggagg tcggggacat cccggcccac cgcatcgtcg gcgagtacgt cttcgcgccg
      541 gccgacgact ggtcctacac gacggtggtc gtggaggtcg acggccgctt cgggttcagc
      601 gccaacttcg agaccgacgc cgtgggctgg ttccgcctcg acgaggtcga cgacctgccg
      661 gtgcacgccg ggttcgcggc ggcctggccc gcgctgcggc gcatcgtcga aggcggttga
      721 gacccggggt tctgtgcgcg acggtctcgt ggaaggaacc agggcgcgcc tccggcgcga
      781 cccatccctg acgcaccacc acgaggagat cgaaccatcg cacgtcgcac gcccttcccc
      841 cgacgctccg agagcaagca ccgcatcaac ggcgagatcc gcgccgagcg ggtccgactg
      901 atcgacgaga ccggggagca ggtcggcgag atgcccctcc ccgccgcgct cgaggcctcc
      961 cgccgggccg gcatggacct ggtcgaggtc gcccctgacg ctcgtccgcc ggtggccagg
     1021 atcatggacc acggcaagta cgcctacgag cagtcggtga aggcccgggc gaagaagaag
     1081 cgcagcggcg gcggcgtcaa ggagatgaag taccgcccca acatcgggga ccacgacttc
     1141 ctcaccaaga cccgcaaggt ggccgacttc ctcaccaagg gcaacaaggt gaagctcacc
     1201 gtgtggctcc ggggccgtga gcgctccatg cccggcctgg ccgaggacgt cgtcgagcgg
     1261 gtcgtcaccg aggtcgacga catggtcgac gacgacatca cggtcgaagc gccggtgcgc
     1321 ggcgacggcc cgcagcgctc ggccgtcatc gccacgtccg ccggcgactg accgaactca
     1381 ggtgaggttg ccgcggatgt cctcggcacc gaggcgacgg gcgtcggcgg tgtggtcgtc
     1441 gacggcctgc tggctctccc gctcggcctc gacccgcttg gagtagtagt cgacctcgcg
     1501 ggcgacggtg gcctcgtccc acccgaggac atcggccatg atcccggcga cgacgggcgc
     1561 ggcgctgact ccccggtcgg gtgactcgat ggacaggcgc gtgcggcggg tcaggacgtc
     1621 gtcgaggtgg agcgcgccct cctgcgccac cgcgaaccgg gcctcgacgg cgaggtagtc
     1681 gtcggtgccg ggcaggcact cggccatcgc cgggtcggcc cggagcagct cgagcagcac
     1741 cggcagctcg gagccgtagc gcccgagcag gcggtcgatg cggtcgaccg gcaggccgga
     1801 ctcctcggcc agctgcgtgc ggcggttccg catcgcctgg tagccggtcg ccccgaccag
     1861 cggcacggtc tcggtgcacg acgccggcgc gtcgacgccc atggccgcca cggcggcgtc
     1921 gaccgcatcg gcggccatca cccggtacgt cgtgtacttg ccgccggcca cggacagcag
     1981 cccgggggtc accgcggtga cggcgtgctc ccggctgagc ttcgacgtgc tgtccgactc
     2041 cccgtgcagc agcggtcgca gtcccgcgta cacgccgacg atgtcgccat gcgtcagggc
     2101 ggggcgcagc cagcggttgg cctggccgag caggtagtcg aggtcggccg cgctggctgc
     2161 gggtcgggcg aggtcgaggt tccagtcgct gtcggtggtg ccgaccagcc agaagtcccc
     2221 ccacgggatc acgaacagca ccgacgtcgg ggtgcgacag atcaggccgg cgccggcgtc
     2281 gatgcggtcg cgcggcacca cgaggtgcac gcccttggac gcccgcaccc ggagggcggc
     2341 cccaccggcc atgtcctgga tctcgtcggt ccacacgccc gtggcgttga cggtctggcg
     2401 ggcccggatg tcgagctcgt ccccgctctc cacgtcgcgg gcgcggacac ccgtcacgcg
     2461 cccgccgtcg cgcaggaggt cgaccacctg caccgaggcc gccactgcgg cgccgtggtg
     2521 gtgcgcggtg cggaccaggc tcatcgtgtg gcgggcgtcg tcgacctggg cgtcggagta
     2581 ggtgatcgcg ccggtgagca cgtcgtcgcg cagcgagggg accagccgcc gggcggcccg
     2641 cttggacagg tgccggtggc gggggagggg gttggagccg aacgcggcga gcacgtcgta
     2701 gagggcgatg ccggcgccga cgtaggcccg ctcccagacc cgatgggtga gcgggtagag
     2761 gaaggtcacc ggtcgggcca ggtgcgggca caggcggttc aacatgaggt tgcgctcgtg
     2821 cagcgcctcg gtcaccaggc cgagctccag ctgctcgagg tagcggaggc cgccgtggaa
     2881 gagcttggac gaccggctgg acgtgccggc ggcgaagtcc cgctgctcga ccagggccac
     2941 cgacagcccc cgggtcaccg cgtcgagcgc cacgccggca ccggtgacgc cgccgccgat
     3001 caccacgatg tcgaagacct cgtcgcgcat gcgggccagg tcctgggccc gctgccgggc
     3061 atccatcggc ggcaccgtgt cgactccgtc caccgggtct tcctaccagt cctccgatcc
     3121 gttctggcag ccacgaggtc acaccgggag gggaccggcg cgcgtgcgcc gaacagtgga
     3181 ccatgacctc gggtgggcag tccggaccgt ctcgtcgtgc gcctcgtcgc cgcacgctcc
     3241 tgcgcacggc ggtcgccggt gcgacgatcg gcgtcacgct cgccggcgcc gcgctcgccg
     3301 gggtggtggc tgcgcccgtc gatgatccgg tcggcgatgc cgtcggcggc atcgccggac
     3361 cggtggaggg cgtgctcgac ggttcgggcg gcggggacga cggggcgatg gtcgaggagc
     3421 tcgccgcggt cgaggctgcg ccgacgtcga gcgggttgcg cgccggcgcg gccaagcact
     3481 cgatcgcccc gcgacccgag gacttcggcg gcacctgggt caccgacccg gctgcctgcc
     3541 gcacgctcga cccgggcttc ctcgaacgcc tcggcaccac gcctgaggac gcggctcacc
     3601 tcgcgcacac cgggacgccg tggccggaga accccgactg catctacatg ggcgggttcg
     3661 gtctcggacc catgaacccg gtgtcctcct tcgacaccga gctcggcctg tgggtgcgct
     3721 cgatcgccgt cggagacggg gccgacacgg tcgtgttgac catcgtcgac ggcgagggct
     3781 ggctctggga ctacgcgaac aagtgcgacg actgcggcgc gaagcagctc agcgaggacc
     3841 tcggcgccga gctcgggatc gacccggccg gcatcgtgat cgccgccacc cacagccacg
     3901 ccgcccccga cttcctcggt ggctggggct tcgtgccgga ctggtacatg acccaggtca
     3961 ccgagacgat ccgtcgttcg gtgcgcgacg cggtggctgc gatggaaccg gcggtcgtcg
     4021 agttcggcga ggtccaggcc cgcgggttca accgtgagcg ccgggacacc taccgcagcg
     4081 ccgaggagca gcaggtcggc tgggtccgtg cctacgtgcc cggcacgccg tccaagaacc
     4141 cgaacaaacc gggtgagccg ccccgggtga tcgccaccct cggggcctac gccgcccacc
     4201 cgacgacgcg ggggaccaac gacggcgtcg cccacccgga ctggcccggc gagttcgagc
     4261 gggcggtcga ggaccgcttc ggcggcatcg gcctccactt catgaccggc ctcggcaaca
     4321 tgtcggcggc cggcgagacc ggtccgggca tcgccgccct cgtccccccg gtcgggcagg
     4381 ggcaggccgt ggcggtcccg tccgtgtccg cctcgcaggc ccggtggcag cagccggtga
     4441 ccaacgtgcc gctcagcgcc ctcgggctgc caggcttctt cgaccggcgc ttcgagccgg
     4501 tgcccgcctc ggtgtccgtc ggcaagggcg acaccgcccc gtgcacctcc gcctccgcgg
     4561 tctcggtgga gctggccgcg tcggccatcc ggatcggcga ccgcctggcg atcaccaccg
     4621 gtcccggcga gctgttcagc aacctcacca acaccatcaa ggagcggagc ccggcggccg
     4681 tcacgctccc cctggcccag gccaacgacg ccctcgggta catgccgcag tccttcgagc
     4741 tggatcccgt cggccagcag ggactcggct tcgccttcag cggctacctg ttcgtcaact
     4801 acgaggactc ctacgccgtc gaccgctgcg tcggcgacat ggtcctcgag acgaccctgg
     4861 acctcctcgg cggctagccg ggctcgacga cctcgagccg gcgggcccgg cccggcctcg
     4921 cctcgtcgat gtcgaccgag aaggagcccg acagcgactc gaccagctcg tcgggcgtgc
     4981 gcgggtcggc gcctgacacc agcagctccc gggcgacgag cccccgggtg tgcttggcca
     5041 tgtgactgac cgcggtgcgc ccgcccggcc cgtcgcgcac gacgtggacg gccaccgtcc
     5101 gctcggcgag ctcgccggcg ggcttccagg ccgccgcgta ggtcgaggag cgcaggtcca
     5161 ccacgacgcc ccggccggcc gcagccggca ggacctcgcc gagcggttcc cgccaggacc
     5221 tggcgagggg acccaccccg gggaggtcca cgcccatcga caggcggtag gacgggaccc
     5281 ggtcgttcgg gtggagggcg ccgaacaggg cgctcacgac caccaggcgg cgggtggcgc
     5341 ggcgcttggc ggcggtgtcg agcgacccga gatcgagggc gtcgtagagc acgccggtgt
     5401 acaggtcggc gacccgcagc gtcggcgccg accacaggtc ggtgttggcc gcgacccggt
     5461 cggcggcgcc cttcggcgcg tcgagcaccg ccagggcgtc ggggcggccc gacgccgaga
     5521 ccagcgcgtc cagcacgcgc cgacgtgtgg cggtgagctc ggggaacgag agcccgtcga
     5581 ggtcgagcgg tgccccgcgg gagccgacgg ccttgccctc ggagggcggc accaggacga
     5641 gcactacagg acggcgatgg ggttgacggg cgacgccgtg ccgcccacca cgatgagcgg
     5701 ggccacggtg agcaggaacg cccaccgatc ctcctcggcg caggcggcgg cgaggtcgtc
     5761 gagatccagg ttgtcgagca ggtgcaggcc catggccacg atgccgaccg tgtggacggg
     5821 cagccgggtg gggacgccgt cggtgggctc gacgtgggac gggtggacgt cgctgacccc
     5881 gtcggagccg agcgtcgcca cctcgcgctc gtggagccac ggcaggcacg acgcgtgcag
     5941 cccggccagc tggtcacgag ggttccagcc gccgtgctcg cggtgccaga cccagcgacc
     6001 ggtgcgcacg agcaggatgt ctcccgtgcc gaccgtgagc ccggccctgg attcggcggc
     6061 ctccaggtcc tcgaccgtga tcggcgtgcc cggctcgagg gcgtcgacgc cccggaccag
     6121 ggggatgtcg aggagcacgc cccgcccgac cacgccgtcg cgcaggtggt gcacgccgag
     6181 ctgctcggcc ccgtgggcgg tgacgacctc ggtggggtgg ccgttgtagg tccggccgtc
     6241 ccagaagatg tggcagaggg cgtcgagatg gctggtggcg tagccgtggg acgcgacccc
     6301 gatccagtcg gcgccgtagc cctcggtgtg ggtgccgatc atgtggtgct cggccggcga
     6361 ggggttctcc ggcgccggca gcgtcggcag cggacgggcg cacgagacgg tgcgaccgct
     6421 gcgcacggtg gctgccgccg cggcgcggtg ctcggcgcgg atgtggttga gggcgccgag
     6481 ctggtcgtcg gcgccccacc ggtcccagtt cgacagccgg gtgcggagcg cgtccacgtc
     6541 cgccgtggtc atcgggggca cggtcatgac cgggcatcct cgccgagcgc gtgctggcgg
     6601 atccaggcgt gcatggcgat gccggacgcc acgccggcgt tgatcgaccg ggtcgagccg
     6661 aacatggcga tggagcacac gaggtcgagg cggtcgcgtg ctgcctccga cagcccgggc
     6721 ccctcctggc cgaagagcag cacgcagcgc cgggggagcg cgacctgctc gagcggcacg
     6781 ctgccgggca ggttgtcgat gcccacgacg ggcaggtcgt gcgccgccgc ccacgccccg
     6841 agcgcgtcca cgtcggggtg gtggtggacg tgctggtacc ggtcggtgac catggcgccc
     6901 cgccggttcc agcgcctccg gcccacgatg tggacggcct cggcgaggaa ggcgttggcg
     6961 ttgcggacga cggtgccgat gttcaggtcg tgctggaggt tctcgatcgc cacgtggaac
     7021 gggtgccggc gctggtcgag gtcggccacg atggcgtcca ggcgccagta ccggtagcgg
     7081 tcgaccacgt tgcgccgatc accgcccgcc agcagctcgg gatcgagccg cgggtcgtcc
     7141 ggccacggct cgggatgcgg tccgacgccg acgtcgccgg gctcctccgg gtcggccgtg
     7201 ccgctcaccg gggttcgctg cggatccccc gcagaccgcg ccaggccagg tcggccacgc
     7261 ggtcggtgac ctcgtcggca tcgaggtcga ggtcgtcgcc cagccagtgg cgggtggcgc
     7321 cctcggcgag gccgacgatg ccatgggcga gcaggacccg ctcgtgctcg gcgagcccct
     7381 cgaccgtgat gaggtcggcg atcacctcgg cgatgctgcg ctcgaggcgg gcggcctcct
     7441 gggcgaactc ggcgtcgcgc cgggtgccgc cgccgaacat cagctggtac tcgtccggtg
     7501 agtccaccac gaagcggaag taggcgcgca ggccacgttc gacctgctcg cggggcccgg
     7561 ccgccgcgct cgtcgcctcg tcgatggcgg ccatgaggcg caggctgacc agctccagca
     7621 gctcgaggta cagcgcccgc ttcgaggtga agtgctggta cagcacgggc ttggtgaccc
     7681 cggcggcgtc ggcgacgtcg ttcatggacg tctggtggaa cccgcgcgac gcgaacacgc
     7741 ggagggccac ctccaggagc tgttcccgcc tgcgggcggc ggggagccgg gacgacatgg
     7801 gacgtggcta ccaccgccac cgccgtcgag gcacatcccg gctgctcccg gcggcccacg
     7861 atttcccgac gccggaggaa ggaggaagga ccgggtcggt cgaaccctcg gtcgtgccca
     7921 gccgcaacag cgcccccgcc gccacgacgc ggcgcccggc ccagccgatc cgtcccgtgc
     7981 cggccacccg gctggccgcc gatctgctcg cggtgaaggc ccgccgacgc cgcttccagc
     8041 agatcctgcc gccccgcgcc ggcgagcgcg gctggtagcg cggcccttcg gctactcggg
     8101 ccgccccagc gtggagcggc cctgcgaacg gtcctcccgc tcggccgcct tcacgccgta
     8161 gccgaagacg atggccggtg cgagcacgat cgacccgatc acgatcgagg ccacgatccc
     8221 gggcgtgatc gcaccgccga agcccacgac gaggccgacg gcgaacagcg cgatggccac
     8281 cccgaacagg ccgtagccga cccgctggcc ggtgcggacg agcccggcga tgcgccggcg
     8341 gcgctcgagc acggggtcgc tccggtcggg gcgggcggga tcgggcacgg caggcatggt
     8401 cgcacaccgt cgggagccca gccgaggccg atcagggggt taccggcggt cagatttctg
     8461 catccggtga acaggagtca gtggcgcctg tcacgaagaa cgttccggaa cacaacaacc
     8521 ggtcgcagga catggcctcg gagtcggtcc tgcgcagtcg aacacggggt ggttcgtgca
     8581 gatgcagctg aacgcgttca agcgaacgct ggcgatgggg gccgtctgga cggtcgccat
     8641 gggtggcctg ctggccacgt cgccggccgc ttccggccag gagggcgtcg agcccgggct
     8701 cggcaacgcc tacgcccagg gcatcaaggt cgatccccgg agcggtcgac tgagcttcgg
     8761 catcacctac ggcatggccc tcgccggcca ccagaacaac gtcgccgtcg ccgagtcccg
     8821 ttccgccgac ctcggcgtga tcggcacgac cctcggcggt gagggctgcg acgggggcga
     8881 cccgaccctg ccgaaggagg accagcccca gccgctcgtg gcccgctcca ccgaggaggg
     8941 cgccggcgag gagaaggtcg agagcgagaa cggcgtcgag aagcgtgcgc tcgcctcggg
     9001 tgagcccctg gcccgttccg tcgccgtcac cgccgcctcc ggcgaggacg ccgtgctgaa
     9061 gatcggcgcc accaagagca cgaccgagtc gggcatcatc gacggcgccc gcgtcgcccg
     9121 ggccgtgacc gaggtcagcg gcgtgagctt cgccggcggc caggtcgtgc tccgaggcct
     9181 ccggtgggag gcggcgtacc agacggcccc caccgaggcg accagcgggt ccttcaccat
     9241 cgacggcatc gaggtcgccg gcgagaagct gccgatcccc gaggagaacc cgttcgagcc
     9301 gctggccgag gccaacgccg tgctcgagcc cctcggcttc cggatcaccc cgccgaccgt
     9361 gcgcaacgag tcgggcatcc agttcgtcga cccgatgcgg atcggcatca tccccgccac
     9421 cgcccgcgag tccgtgctcg gccccgtgtt caacggcatc cagccggtgc gcgagagcgt
     9481 cttcgacgcc atcatcgagg ccgactgcgg caacgccgcc tacatcacgg tcctcgacct
     9541 cgtgctcggt tcggtctccg gcgccggttc gctcggcctc gagctcggcg gcgtcaccgc
     9601 cacctccaac gagatcgcct tcacgtcgtt cctcggcagc ctcggcgatg ccccctcgct
     9661 gcccccgctg agctcgggcg ccggctcgtc gccgtcgctg tcgggcagcg gcagctcccc
     9721 ctcgctgtcc ggtgggtcgt cggcccctgc cggtgctgct gccaccacgc ccccgaccac
     9781 cgtggcctcg gccccggcgg acggcggcgg tggtgagcag gcggcgatcg ccgacgtcga
     9841 acctctcacc ggcagtcgtg gcggtgccct cgcgggcgtc ggcctcggcg gcctcgcact
     9901 cctcgctgca ctcgccgccg ccgatcgggc ccagatgcgt cgtgcgcagc gttccatcct
     9961 catggaggtc agctgacatg cctgcgccca ccccgcaacc caccccccag ggatcgttcc
    10021 cggtcaacaa gcgcatcctg cgcggctacg gcccgctcgc cgtcttcgcg gcgatgctcg
    10081 tcctgatggc gatgatcgtc cccacgacgg tcccgaacca gcaggtctcc gccgacggcg
    10141 gcaccccggg tgccggccag ggcttcagca cctccggcag ctcgtcgggc tcgtcgggca
    10201 cctcgtccgc cggcaccgcc accaccgccc ccgccggcgg tgccgacccg gctgctgccc
    10261 cggccgaggg cggcggcgag ggcgtcgcca gcgacggcga gggcagcggc atcgccccgg
    10321 tcgagggcgt ccaggcctgc cccgaccgcc aggagcagat ccccggcgac ccctacagcc
    10381 cgccctgcgt cgcctgggcc ggcggcaaca acggcggcgg caccaccaag ggcgtcaccg
    10441 aggacaccat cctcgtgtcg taccgggtcc tcggtggcga gaagggcttc cagcagacgc
    10501 tggccgagct cgccggcgcc agcctgtcgg acacgcccga gaccatccag aacacgatca
    10561 cggcgttctc cgagtacttc aaccagcgct tcgagttctt cggccgccag atcaaggtcc
    10621 agttctacga cgggcagggc tccaacacga acgagctgct cggcaagggc cgcgaccggg
    10681 ccgaggccga cgccacgaag gtggcggagg agatcggctc cttcgccgac ctgtccgcca
    10741 cctccgagcc ctacgccggc gcgctcgcct cccgcggcgt gatcggcttc ggcaccccct
    10801 acctgtcccg tgagtggcac gagcagcggg cccccttcgc ctggtccctc gccaccgacg
    10861 gcacccgggt gtccgagctg gccgccgagt acggcaacaa gcgcctgcac ggcaagaacg
    10921 ccgacttcgc cggcggcaac ctcaagggcc agccccgcaa gttcgccacc atcgccccgg
    10981 agaactcctg gtaccaggag tcggtgaaca acgcccggcg catcacggag gaggccggca
    11041 acgaccccgg gatgaacgtc aagtaccagc tcgacctgtc caccatgtcg agccaggcca
    11101 acaacatcat cccgaagctg aagaacagcg gggtcaccac cgtcgtgtgc ggctgcgacc
    11161 cggtgctgcc ggtgttcctg tccggcgtgg ccaaccggga gcagtacttc ccggagttca
    11221 tcatcgccgg caccgccctc accgacgccg acatcgtcgg ccagctgtgg gagccggcct
    11281 tcgccaagca ggccttcggc gtgtcgtcgc tcaccgactt cgtgccgccc acccagacga
    11341 tcgcctacga ggcgttcaag acggtgcggc ccaacgagga gccggcgttc tccgtcgacc
    11401 tcatctacta ccagatgtac atgctggcca tcggcctgca gggcgccggg ccgaacctga
    11461 ccccgcagtc gttctccgac ggcatgttca actacccggc ccgctccggc ccggtcggct
    11521 tgtggaagtt cggccccggt gaccgcacgg ccgccaacga cgtgcgcgag atctacttcg
    11581 acatcaacgc catctccccg tacaacggca agcagggcgc ctacatcagc accagcgccg
    11641 agcgctggga gaccggccag atccccgagg gcaacccgcc gatcccgggc ggcgcgtgat
    11701 gaccgccgtc ctcgaccgtg tcccgggcgc cgcggcgctc gacgagatga gcaccaaggc
    11761 caaggccggg ctcgccatcg ctgcgctcgt cgtcgggtac gcgctgctct acgtcctgcc
    11821 cggcaccggt gacttcctcc gggacaaggc cccctacggc gtgctgttca tcggcacggt
    11881 gcagggcacc accaccgccc tgctggccat gggcctgatc ctcatctacc gcacgaaccg
    11941 gttcgtgaac ttctcctacg ccgcgatggg tgcgctgccc ggggccttcg ccctcggcct
    12001 ccacctggag cagggcctgc cgttcttcgc ggcgctcgcc ctcgccgtgg tcatcggcgt
    12061 gggcgtcggg ttcctcaccg agctgctgat ccggcggttc aagggggcga gccggctgat
    12121 cctgaccgtg gcctccatcg gcctcgccca gatcttcggc gtgctgagcc tgctggccaa
    12181 caaggccatc gggttcatct ccctcgccgg cgggttcacc atccccctcg acgtgcggtt
    12241 caccctcgac gtcaagacca tgaccggcga cgagatcctc atcatcgccg tggtccctgc
    12301 ggtcatcgcc ggcctggcct ggttcctgct caagaccgac gccggcgtgg ccatccgtgc
    12361 cgcagccgag aacgaggacc gcgccctgct gctcggcatc cccgtctaca agctcggccg
    12421 catcgtgtgg atgatcgccg gcggcctggc cgccctcacc tacgtgctcc aggccccgtc
    12481 ggccggggtg tcgccctcgg tggtgtcggc cggccccgcc gtgctgctgc ccgccctcgc
    12541 cgccgcggtc gtcgcccgcc tcgagtcgct gccgatggcc ttcgccgccg gcatcggcct
    12601 cggcatgctc gaccagctcg tgcgctggaa cggcaccggc agcccgtcga tcaccaccat
    12661 gttcttcctg gccgtgatcc tgatcgccct gctcctgcag cgggcctcgc tgtcccgggc
    12721 catgaactcg gagagcgcgg cgttcagctc cgaaggggtc atcaagcccg tccccctcga
    12781 gctgcgccac ctccccgaga tccgcatcgc caaggtggtc atcggcgtcg ccgtgctggc
    12841 cctgctggtc ttcgtccccc tgtcatgggg cccgtccaac cagctgctcg cggccatcgc
    12901 catggtctgg gccatggtcg ccgtgtcgct cgtgatcctc accggctggg gcggtcacat
    12961 cagcctcggc cagttcgcca tcgccggcat gggcggcctg ctcgccggca acgtcctgca
    13021 gcgtggcgac atcgactact tcgtggtgct caccggcgcc ggcctggtcg gggcgctcac
    13081 cgcgctgatc gtcggcctgc ccgcactgcg catccggggc ctgttcctgg ccgtgagcac
    13141 cctggccttc gccgtggcgc tcaacagcta cgtgctcaac cgggacaact tctcctcgat
    13201 catccccggc gacgtcaccc gcccggtgct ctgggagcgc tgggacctcg agcggggcta
    13261 cccgatgtac gcggtctgcc tgtcgttcct gctgctgtcg gtgctcgccg cccgcagcgt
    13321 ccggggcacc cgggccggtc gggtcatcat cgccacccgc gacaacgagc gcgcggcgga
    13381 ctcggcctcc gtgcccacga cccggatcaa gctggccgcc ttcctcgtgg ccggcaccat
    13441 cgccggcatc gccggcgcac tgcacgtcac catcctccac ggcctgaacc agggcaccta
    13501 cagcccggag atgagcctcg aggtgttctc caccgcggtc atcggcgggc tcagctcggt
    13561 gtcgggtgcc atcggcggcg tgctgctgtt ccagtggctc gggtccatca ccgccctcgg
    13621 cgacgtccgc cagctgatct ccggtgccgg cctgctcatc gtgctgctgg tcttccccgg
    13681 cggcctggcc cagctcgcct gcaacgtccg ggaccggttc ctgcgccgca tcgccgagcg
    13741 ccacgacatc ctcgtgccct ccctcgtcgc cgacaagcgg gtcgaggagg aggacaaggg
    13801 cgaggacgag agcctcctgc gaggcgccct cggcggcgac gacgaaccca ccaccaccac
    13861 acacgacgag ctcgtgggga gcgcccgatg acctccacca acggacgaag cgtcacgacc
    13921 agccgccgcc gggcccccct gcgggccgag gccggcgggg tgccggacct gggtaccgac
    13981 gagaccctgg tgctcgacac ccccgtcgcc aacggccacc gaccggcgga cgccgccgac
    14041 cgggccgcgg cgccggaggt cgggaccgag acgatcctga agtgctcggg catcgacgtc
    14101 agctacggcc cggtccagat cctcttcggc gtcgacctcg aggtgaagcg gggcgaggtc
    14161 gtggccctcc tcggcaccaa cggcgccggc aagtccaccc tgctcaaggc catctgcggg
    14221 ctgaaggccc ccaaggccgg cacggtcgag ttcaagggtg aggacatcac cggcctgtcc
    14281 gccgacctgg tcgccgacaa gggcgccgcc ctcatgcctg gcggcaaggg catcttcccg
    14341 acgctgaccg tggccgagaa cctccgcctc gcctcctggc tcatcaagga cgacccccag
    14401 cgggtgaagg agtccaaggc cgaggtcctc gagctgttcc cgatcctctc cgagcgctcc
    14461 aaccagctgg ccggcgacct ctccggcggc gagcagcaga tgctcgccct cggcggcgcg
    14521 ctcatgacca gccccgagct gctcatgatc gacgagctct ccctcggcct cgccccgacc
    14581 atcgtcgccc gcctcctcga ggtcgtcgac gagatccacc gccgcggcac caccatcatc
    14641 gtggtggagc agtcggtcaa cgtggccctc aacctggccg agcgggccgt gttcatggag
    14701 aagggcgagt tccgcttcac cgggcccacc cgggagctcc tcgaccgccc cgacatcctc
    14761 cgctcggtgt tcatcgccgg cggcaaggcc gacgaagccg acgatgcgga caaggccgag
    14821 acgggcgccc agcccaagcg ggtgtcgacc gtcgccgagg acgcccgggc cgtgctccag
    14881 tgccggggcg tcaccaagcg cttcggcggc atcaccgccg tcgacagcgt cgacgtcgac
    14941 ctgcacgacg gcgagatcat cggcctcatc ggccacaacg gtgccggcaa gaccaccctc
    15001 atggactgca tctcgggctt cctcgccatc gaggacggcc gcatcggcat ccagggcctc
    15061 gacgtcaccg actggcagcc ccacgagcgg gcccgggccg gcctcggccg ctccttccag
    15121 gacgcccg
//
LOCUS       DATISU010000025        25679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_140826, whole genome shotgun sequence.
ACCESSION   DATISU010000025 DATISU010000000
VERSION     DATISU010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 25679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25679
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140826"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VLR27_01220"
     CDS             <1..888
                     /locus_tag="VLR27_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HSP02089.1"
                     /translation="ALLRAGRFDRQVVIPLPTAGERLAILEVHARGKKLAADVDLDLV
                     ARGSPGMSGADLANLLNEAALIAVRRGADEIGAGDLDAARDRVLLGVPRGALVLTSEE
                     KRTVAYHEAGHAVLASVLPNSDPVHKVTILPAGIALGATQQVPVTERQLRRRGELDDI
                     LAVRLGGRAAEELVFAEVTTGAADDLTSATQLARSMVREWGMSERLGEMAWGSAGPVF
                     LGEDLVHTRDYSDETARVIDEEVERILEEQAQRARTELQQSMDALHAVASELLERETL
                     EADDIARILHPPGPVAAAG"
     gene            962..1165
                     /locus_tag="VLR27_01225"
     CDS             962..1165
                     /locus_tag="VLR27_01225"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HSP02090.1"
                     /translation="MSDVARVTEISARSTVSFEDAINVGVERASETLRNVTSAWVKEH
                     QVRVEDGQVAEYQVNLLVTFVLE"
     gene            1169..2509
                     /locus_tag="VLR27_01230"
     CDS             1169..2509
                     /locus_tag="VLR27_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HSP02091.1"
                     /translation="MFSDRVDAGKQLAGRLDHLRGDDVVVVGLPRGGVPVAAEVARAL
                     GAPLDVIVVRKLGVPFQPELGMGALGEDGVKVLNPDVIVMAGVTDRELAAVEARESAE
                     LRRRGERFRAGRERVPLTGRVVVVVDDGVATGSTARAACEVARAQGASRVILAVPVAP
                     ADWTRRLASAADELVCVATPSPFFGIGQFYDDFSQTTDDEVVRHLRAAAAPATPTSPE
                     PGRPVAGDDPAIRDEEVEVEAGPFHLGGHLTVPETAHAVVVFAHGSGSSRHSPRNRYV
                     ATMLNDAGLATLLFDLLTPAEESDRANVFDIELLGQRLAAVTAWVHQQPDVAGLPVGY
                     FGASTGAAAALAAAAAPDVDVFAVVSRGGRPDLADRLLGSVRAPTLLIVGGRDDVVLG
                     LNREAQVRLRCPSQLTVIPGATHLFEEPGTLQAAAAAARDWFLHHAPRARTVAS"
     regulatory      2585..2692
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2715..3476
                     /locus_tag="VLR27_01235"
     CDS             2715..3476
                     /locus_tag="VLR27_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP02092.1"
                     /translation="MVMTAAPNRVVWVDLSTTDLESAIHFYERIFGWHYEEHDSGMQT
                     NVVARVAGGEVGGMMAQPPEQAASGVPSAWTVFVGCGHLENALTWARELGGSVLQAPV
                     SIPGGARVAVIADPSGAALALAEGALAVGTAVRDEPGALAWVECQSRDLARSRGFYEE
                     LFGWQSEEGTDGYVVFTADGERAGGLMAMPRTVPAEVHSHWLVYFAAEVAATADAVTE
                     LGGTVVVPAHEIDEGRFAVLEDPTGAVFAIYERSP"
     gene            3513..5123
                     /gene="groL"
                     /locus_tag="VLR27_01240"
     CDS             3513..5123
                     /gene="groL"
                     /locus_tag="VLR27_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797913.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HSP02093.1"
                     /translation="MHTELLFRDEARTKLLAGANAVADAVRATLGPEARSVLLERKWG
                     TPLVCDDGVTITKQIKLKDQVENLGAQLLRNAASETGDAVGDGTTTSTLLAHAIFSEG
                     VRNVVVGTSAVGIKRGLQRGLDAAIGSLHELSRPVKDRTDTEHVATVSAHNDPEIGRL
                     VADAVDQVGPEGVVEVEEAKGTETMLEVVEGMQLDKGYLSPYFVTDPERMEAVVDRPL
                     VLLVDRKITAVNELLPVLEEVAKSGRGILIIAETVEGEALAALVVNRLRGSLAAAAVK
                     APGFGERRKAMLEDIGILTGTRPVSEELGRKLEAVTVDELGSAERVVITKDTTTIIGG
                     GGDPGAVNGRCDELRRQIKDTTSDYDREKLEERLAKLSGGVALIRVGAISEAELKRQK
                     EAFDDAISSTKAAVAEGIVPGGGAALLRTIDAVEAEEQRCEGVERVGVHILRNALEIP
                     ARQIAKNAGIDDGPVVEKIRNGTGFFGFDARTKDFAELDERGIIDPTKVVRIALQNAV
                     AVAGVLLLAEATMVEVEEEAPPQLPEFG"
     gene            complement(5323..6003)
                     /locus_tag="VLR27_01245"
     CDS             complement(5323..6003)
                     /locus_tag="VLR27_01245"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSP02094.1"
                     /translation="MRCEMRVRDVMTTPAITVSPETPFPELVDRLLSHGVSGLPVVDT
                     SGVMVGIVTEADLVPKEAYGGHKRRLIQVLADLVAGGETRWAIKARGRTAAHVMTRDV
                     TTVGPDVQIRVAARRLVEAGVKRMPVVDETGHVLGVVSRRDMLQILHRSDEEIRHGIE
                     AALVDPRRAPEHLDVEVAVEDGVVTLTGTALFPQDVAVLTSMIWRIPGIVDVSNHVQS
                     REPDPKPY"
     gene            6461..7945
                     /locus_tag="VLR27_01250"
     CDS             6461..7945
                     /locus_tag="VLR27_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSP02095.1"
                     /translation="MRRGAGATFWPCPASLPSASTRPMSTASIHRLAIFEQGPSARDS
                     GRPVGAVSSSSGSMPTGRGRRHLKRERRTAVAVRRQQTVFAFAPTGMRVGGWSAVTGP
                     DPEHHRVGDLRLWSLVAGRASDGVMSPEGLHIGLIAPPWVAVPPPRYGGTEVVIDHLA
                     RGLVAAGHRVSLFTTGDSTCPVEKHWVVPEAVGTTGSLTVELGHVQAAYEQLAGCDVI
                     HDHTLLGPLWAVVDGVQIPMVTTNHGEFTPELTALYRSVGRHVAVVAISHHQASTAPT
                     VPVSQVIHHGIDVDTFPVGRGDGGYVVFLGRMHPDKGVHRAIAVARTAGKRLLIAAKM
                     WEPAEHRYFAEQVEPLLGPDVVYLGQVGGADKHDLLAGAEALVNPIRWPEPFGLVMIE
                     ALAAGTPVLAFPEGAAPEIVDHGRTGFLCRNEADMAARLVTVGAIDRAACRHAAESRF
                     STRRMVDDHIALYERITAAHPPVVDLRDASHRGTASGPRLRNLA"
     gene            8020..8424
                     /locus_tag="VLR27_01255"
     CDS             8020..8424
                     /locus_tag="VLR27_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSP02096.1"
                     /translation="MPAWFGTRRLFDWPESWTELMGAEPQLKVEEFREDGQLVVRAEM
                     PGIDPDKDVEITVAGDILHLRAERRSETKTEGASGYRSEFHYGSFSRSLRLPAGATEA
                     DIKATYTDGILEVRIPIDTATAEAKKIPVSHG"
     gene            8790..9023
                     /locus_tag="VLR27_01260"
     CDS             8790..9023
                     /locus_tag="VLR27_01260"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HSP02097.1"
                     /translation="MMGEKGDQVKGRAKEAAGVLSGDADLESEGKADRRAGEAKEKFG
                     HAKDKVEAAVEKVEDKAGEVIDKAKDALHRKRT"
     gene            complement(9132..9908)
                     /locus_tag="VLR27_01265"
     CDS             complement(9132..9908)
                     /locus_tag="VLR27_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSP02098.1"
                     /translation="MITVLAAIDDSPTAECVLRAAHIWSSLLTAELRAVHVNEDGPPD
                     VLASAARAGLDVQVLDGDPGRVLLDLASDPSVGLVVIGSRRAVDGARPAGHIAIALVE
                     HAGVPVLVVPPEWVSTTETRVRKVLVPLEGSAATTSPIAAILRQLADAGVALLAVHVF
                     APGDVPRFSDHSLHAEEGLAASFAERWCTEAPTEVRLLVGAEPETVMEAVDAERPDAV
                     ALTWAQDLSPGHAGVVRAALERSHRPVLLVPQGAVAPPGT"
     gene            complement(9905..12016)
                     /locus_tag="VLR27_01270"
     CDS             complement(9905..12016)
                     /locus_tag="VLR27_01270"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HSP02099.1"
                     /translation="MVPDLIDGAEPQHEGPPSPETLGRPSAWTLQVGDHRGHHGTLAA
                     LANGVIGVVGDVGPDPVGSRATFVAGAYGVAADGMVRPLPGPAWTAIGGDPPGTAWAL
                     DLRDGLLSRIDRRGRTTAARLVSVVRPTIGVLWAAGDVGGGWPAPLAPPDVPRALCAD
                     HTCEISSGGDGTDTAVVRGGREVVVATASQQVRRPHRMERIVSMARGPGAEGTARDAL
                     DEAVALGVDGLVAEHRAAWTSRWEDADIEIVGDPEAQLAIRFALFHLLSCAAPGDESP
                     VGARGLTGLAYAGHVFWDTDVYILPALAAVAPAAARSILEYRIRRLPAARARARTGGL
                     AGARFPWESADSGEDVTPRFGRDMHGEVVPITTGQLAEHITSDVAWAAVHYLDWTGDP
                     SFLASGGRDLIVDTASYWMDRSQLDEWGLAHVYDVTGPDEYHECVDDDAYTNLMVRWH
                     LGQAAHLVAAEGDDERASALRRRAAALVVGRRAGGRHEQFAGFDQLEAVDLADLGPTP
                     VAADIVLGRARTVRSKVLKQPDVLMAHHLLPTAFSPAELQADLDGELPLICHGSSLSP
                     AVCAAVLARAGRADEALELFRLASRIDLDDLTGSTSSGLHLATMGGIWQAVVFGFVGV
                     IPGGRTLQVDPHLPTSWESVRVRFRHQGVLVELHVDHRTVSVEAEEPIPVRVQGVQLT
                     APVSQAIRHDHLRDQRAEMTS"
     gene            complement(12032..13171)
                     /locus_tag="VLR27_01275"
     CDS             complement(12032..13171)
                     /locus_tag="VLR27_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSP02100.1"
                     /translation="MPDRLRIGMIAPPWVPVPPRGYGGTELVVDELARGLERAGHHVV
                     LWTTGDSTCPVERRGVLDRAAGTEASTDVEVHHVADAYDSLRDCDLIHDHTFLGPLWA
                     VNEPRSAPVAATVHGTFTPASRALYGSVAGRVSVVAISAHQRSTAPEVDVRAVIHHGI
                     DPPSDWRRTGDGGYLAFLGRMNPDKGVVEAIRIARAAGRRLVIAAKMWEPAERAYFDA
                     EVAPLLGPDAEYIGHVSGPAKHELLAGADALLNPIRWPEPFGLVMIEAFGAGTPVITL
                     AGGAAPEIVEHGRTGFVCTDESEMVAAIGRVEEIERWACRRAAVTRFSAERMVARHLE
                     LYRRMLDPATPVRSRSVRIGRPRPADPPPSRRPGGSGAPDRAHSA"
     gene            13612..15933
                     /gene="ppsA"
                     /locus_tag="VLR27_01280"
     CDS             13612..15933
                     /gene="ppsA"
                     /locus_tag="VLR27_01280"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HSP02101.1"
                     /translation="MTDPTTAISWFDELSRTDTATAGGKGANLGELASAGLPVPDGFV
                     VNARGYLGAMDEGGVRDELQAAFATACGEASTPTALADAAERLQSLVRKAGVPAGLRS
                     EVLQAYHRLGDRVPVAVRSSATAEDTAGTSFAGMHETFTDVVGDDAVLARLVDCWVSL
                     FGQRAISYRASQRMTDEPVIAVVVQRLVAADRAGVLFTADPSTGDRSRLVIEGAFGLG
                     EVVVSGAVEPDTYVVGKDGPRIQLARIGHKTHRLVAEPDGSVTRVDLDDTEADARVLV
                     DDEVLDLARLGLQVEDHYGGEPQDVEWAIAAGRLYLLQARPITTLAAPEAASPPEAET
                     ADAGVPLVRGLSASPGVVAGSVRVLASPAEGHVLKLGEVLVAPMTNPDWVPTIRRAAA
                     VVTDGGGMTCHAAIVSRELGVPCVVGARDATTRLRDGDIVTVDGGAGEVYEGDRVGAR
                     TVPATASAPSVVPSGEALATRIYVNLAIADHAEAVAAQPVDGVGLLRAEFMITDALGG
                     VHPRALLERGGRAEFLDRMSASLLRITRAFAPRPVIYRTIDFRSNEFRGLEGGAAFEP
                     VEENPMIGYRGCFRYVREPDLFALELELLGRVREETPNLHLMIPFVRTTWELEACLEA
                     IDASVLGRQRGLHRWVMAEVPSVAYRIPEYAAMGIDGVSIGSNDLTQLMLGVDRDSAV
                     CAELFDESDAAVLDAIGRIIGACQAAGITSSLCGQAPSNKPAFAEYLVRLGIDSISVI
                     PDAVDAARRVVASAERRLLLDAAREAGRRPAPR"
     gene            15930..17417
                     /locus_tag="VLR27_01285"
     CDS             15930..17417
                     /locus_tag="VLR27_01285"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSP02102.1"
                     /translation="MTPARPPPQAGGTEPYAMAVETHTGAVLLVGDLVYKVKKPVSVG
                     FLDWTDRADRVRAIEDELVLNRRLAPDVYLGTGLLPVPGGEDEPVLVMRRLPADRRLS
                     TLVEQGRDVDDALRALAHQLAAFHLRGATTTAAAAAAGPAETLRRWEVNHERMRPAFG
                     EWIDGEAAELLLERARAYLRGRATLLEERVSRGWARDGHGDLLAEDVFCLDDGPRVLD
                     CLDFDERLRVGDVLADMAFLAMDLERLGRADLGWRVLELHREMTGDSWPPSLAHHHVA
                     YRAQVRALVSCVRAAQGDAAAGVQAAPLLDIAGRHAAAARVRMVLVGGAPATGKSTLA
                     AGLGDRLGATVLRSDEIRKELAGRSVRDDCGAELGAGLYSEAWTDRTYGELLRRAGRL
                     LARGECVVLDATWAEHQRRRAARVVAEATSTDLTELRCVVDVETARHRAGARARAGMD
                     VSDADAAVASALAASFDPWPDAPEISTASTPETALDAALVVLERR"
     gene            complement(17426..17794)
                     /locus_tag="VLR27_01290"
     CDS             complement(17426..17794)
                     /locus_tag="VLR27_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02103.1"
                     /translation="MGARSEPGVALVSASRLTALDAEPLGVGGGAVHRTTWTDGRSSA
                     GVMHIDAGHHLGEHTHRRHHHHVWVVTGSTRVLGEDLPAGSYAHIPAGVEHDFEAVGD
                     EGCTVFYLYLEQGQEGPATD"
     gene            complement(17867..18127)
                     /locus_tag="VLR27_01295"
     CDS             complement(17867..18127)
                     /locus_tag="VLR27_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02104.1"
                     /translation="MQIEASRRRRTWTETDRGMLVLVKLVMADDWSAGRAAAQLVRLI
                     RDPRVLGRLTVRVRAAQSDRPSEFGERAEQTLLRAEAQLGAA"
     gene            18242..19519
                     /locus_tag="VLR27_01300"
     CDS             18242..19519
                     /locus_tag="VLR27_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HSP02105.1"
                     /translation="MTSVPTGIVRGPDQTDESSLERLPLAVLELDGDLRAVDVNQGWV
                     QLCGRSQHDSRGSLWWQSVDPRDRAALQRALSSPAPSDDVRAEHRVVGAGDPRWTRWW
                     WRHDGDRTWACVADVDADWSRTRDLWRRATHDPLTGLANRTEFMTLLERALDRAIDHP
                     ALAVVFVDLDHFKGVNDSAGHRAGDAVLAAVAHAIVEAVRPFDVAGRLGGDEFAVLCP
                     GLTSEDETTALATRIRDAVAASEAVTGIPISGSAGVAVSVAGDGSADSLVARADQAMY
                     RDKSARRPPSPSGGGRPAPLSADVGEQHVAAVLVPMLFRLGLSLHAVADAADDLTASR
                     VRDVVEELDDVIASLRHAAFAHLRDTADAASVRVAFRRVESAIAGAEHAVRQEWAKTA
                     TSASDDPGLALRLVRSSRLLRAAERALEPDALG"
     gene            complement(19466..19927)
                     /locus_tag="VLR27_01305"
     CDS             complement(19466..19927)
                     /locus_tag="VLR27_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSP02106.1"
                     /translation="MARSREVLLVEDDPAWALLTSEAFADAAPEVSVQRCSTGRDALA
                     RFRVEPWPDAVLLDLNLPDLDGVDVLRALRGLPGAQDLVVVVLSASRAAADRSLAEQL
                     GATAYREKPTTYSELLALARDVAHGATEAFPPDRSTQRVWFERSLRGPEQP"
     gene            complement(19929..22061)
                     /locus_tag="VLR27_01310"
     CDS             complement(19929..22061)
                     /locus_tag="VLR27_01310"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HSP02107.1"
                     /translation="MTSRERKRDGPRWTRSLAVLAPLAVVAAGAVATALAADYEDRVR
                     ERDLRQRADLIGDALADELSALLETQIGGAAATAAALSVDPDLDRAAFARYAGGIADQ
                     LPTLLGVGYVRRVAATDLDSFVAGARADGAPDFELQDLSTGPEHAILLYNEPTAELRP
                     SWGVDVTSVDEAARALQRATDGGRTAVTEQLVLAVDRDVAPAERPVGYVAYAPVFAPG
                     APTGSVEQRRQAVVGWSNLPFRAQELLDELRLPTGVRVVLRDGEPDRPAIAAAGPVPD
                     GATARTLQPLRSPNAEIGWVLEATAPSSIVQPQLDRSAAAIAAGTSLTALLAVLVGLV
                     ARGSTRWAGTAKRATQTLIESEERLRATIDAAPDLLLVVDAAGTIMSASPRARDLLGT
                     DAAALGGEHISQLLPAIHDPASIGDRTEHQVRRNDGSRVVVELAARPLRPEDPASPRV
                     VVVRDVTAQRQARRQLRARSRQLERSNQDLQAIATIAAHDLSEPLTVIGGYADLLVQK
                     YQVGSTVDDAAATHLRTIAGGAQRMRAMLDALLHMSSMETHEPPAPVPLDQVVRDALA
                     NTESALRSCDAEVVVGPLPTVVGHRDLLVELMQNLVANAVRHADADDPLRIGIESVRD
                     GARWRITVSDNGSGVPPEQRRRIFQAFRRLDGNRPGLGIGLSIARRIVQLHGGRIKVE
                     DAPDGGAAFTFTLPAVHPDGSTSASEPR"
     gene            complement(22058..22714)
                     /locus_tag="VLR27_01315"
     CDS             complement(22058..22714)
                     /locus_tag="VLR27_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP02108.1"
                     /translation="MGEPLAMLCVDDQRTFLDALRIAITSDPVGDLRCAGGATSVAEA
                     LEILDHTAVDVVLMDFDLPDVDGIDGTRRVKSRHPDVRVVILTALADLDVYLRAAAAG
                     ADGFIAKDTPIDQLIRMIRMDARGLTLDESILRSLRARVGDEGSISGRTWRPDLTLRE
                     KEVLALLAAGIDATNIARQLGITVHTCRGYVRNVLTKLGAHSQLEAVAVAHQAGLLLR
                     "
     gene            complement(22707..23300)
                     /locus_tag="VLR27_01320"
     CDS             complement(22707..23300)
                     /locus_tag="VLR27_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02109.1"
                     /translation="MDSNDQAPRRPAKPEDDVLGAVVALCRGLDDAATAQEILRRALP
                     IVRSVLGDRVAFDLRAVAPNGGSSGAADVVVPLQADGRHFGDLRASGIDARSTVAHVF
                     ASAAGHVIGTALLLHQERAERAGSAASTTDVLSHRLRGALATIGVAASTLRVRGGELE
                     PAMQLQLLLDIEANVAALRDVVDALGTGTDAPGGHDG"
     gene            complement(23361..25541)
                     /locus_tag="VLR27_01325"
     CDS             complement(23361..25541)
                     /locus_tag="VLR27_01325"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HSP02110.1"
                     /translation="MGTEPSSADAPSPFAGTTPVPSSATVGVVTLVEGQSFCVSGRAG
                     DITADFPHGLFLLDTRIVSSWSLQLNGHRVEPLGVDVHEPFGATFLGRSAPAEGHADA
                     DVVVLRHRHVGQGMRERIVVRNHGLRSAPMALHLTVDADFADLFAVKEGRARALGQIR
                     SEEESGSLHFEQRSEAGSKDVRISASPPGTTGSDSIAWEQTLEPGASFEVCIQVAATI
                     RGIAVEPRFLCGQQDHESLPRQRMTTWHATLPTVETASAPLAQTLARSAEDLGALRIF
                     DAEHPELPILAAGAPWFMTVFGRDSLLTAWMTLIADPSLAHGVLETLARFQGRDVDPK
                     TEEEPGKILHEMRFGSTTNLALGGGERYYGSVDATPLFVMLLGELRRWDARDELVDRL
                     LPHADRALAWIDEHGDRDGDGYVEYQRSTEAGLANQGWKDSWDAVRHRDGELATTPIA
                     LCEVQGYVYAAYVARVHLAREAGDAATAERYAAKASELRRRFNEDFWSDEHGWYVLAL
                     DGDKRPVDVLSSNIGHCLWSGIIDPARAGVVADRLLSTDMFSGFGVRTLSASAAAYNP
                     VSYHNGSVWPHDNALCAAGLARYGFVDAAHRIIEAQLDVARAHDGRLPELFTGFSRDE
                     FAVPASYPASCAPQAWAAASPMLWLRTLLRFDPPASDGTIWLDPQLPDGFGDLTVSGI
                     RVGPHDLAVTVSDGHVHVDGAADLRVVRHARPPLSEVVHAPPGP"
BASE COUNT         3644 a         8950 c         9409 g         3676 t
ORIGIN      
        1 gcgctgctgc gtgcgggccg gttcgaccgt caggtcgtca tccccctgcc gaccgcgggg
       61 gagcgactgg cgatcctgga ggtccacgcc cgtgggaaga agctcgccgc cgacgtcgat
      121 ctggacctcg tcgcccgggg cagccccggc atgagcggcg ccgacctggc caacctgctc
      181 aacgaggccg cgctgatcgc ggtgcgtcgc ggcgctgacg agatcggggc tggtgatctc
      241 gatgcggccc gtgaccgggt gctgctcggg gtgccccgcg gtgcgctcgt cctcacgtcg
      301 gaggagaagc ggacggtcgc ctaccacgaa gccggccacg cggtcctcgc gtcggtgctg
      361 cccaactccg acccggtgca caaggtgacg atcctgccgg cagggatcgc gctcggcgca
      421 acgcaacagg tacccgtgac cgaacgccag ctgcgacggc gcggcgagct cgacgacatc
      481 ctcgccgtcc gcctcggtgg tcgggccgcc gaggagctcg tgttcgctga ggtcacgacc
      541 ggcgcggccg acgacctgac gtcggcgacc cagctcgccc gcagcatggt gcgcgaatgg
      601 ggcatgtccg agcgcctggg agagatggcc tggggctcgg cgggtccggt cttcctgggc
      661 gaggacctgg tccacacccg ggattactcc gacgagacgg cacgggtcat cgacgaggag
      721 gtcgagcgca tcctcgaaga acaggcacag cgcgcccgca ccgagctgca gcagtcgatg
      781 gacgcactcc atgccgtggc gtccgagctg ctcgagcgcg aaacgctcga agccgacgac
      841 atcgcccgca tcctccaccc gcccggcccc gtcgcggccg cggggtgacc ttcgccccta
      901 caccgggcgg gagggtccgc ggttcactcg cgtgcacgac ggcgaacaag ggggcaggaa
      961 catgagcgat gtcgcacggg tgaccgagat ctcggcacga tccacggtga gcttcgaaga
     1021 tgcgatcaac gtcggggtcg agcgggcgtc ggagacgctg cggaacgtga cctcggcgtg
     1081 ggtgaaggag caccaggtgc gggtggagga cggccaggtg gccgagtacc aggtcaacct
     1141 gttggtcacc ttcgtcctcg agtagctggt gttctccgac cgcgtcgacg ctgggaagca
     1201 gctggccggg cgactcgacc atctccgggg agacgacgtg gtggtcgtcg ggctgccccg
     1261 gggtggtgtc ccggtggccg cagaggtggc tcgggcgctc ggtgcgcccc tcgatgtgat
     1321 cgtcgtccgc aagctcgggg tgcccttcca acccgagctg gggatgggcg cactcggtga
     1381 ggacggggtc aaggtcctca accccgacgt catcgtcatg gccggggtga ccgaccgtga
     1441 gctcgccgcg gtggaagccc gggaatccgc tgagctccgc cgccgtggcg agcggttccg
     1501 agccggccgg gagcgggtcc cgttgaccgg tcgggtggtc gtggtggtcg acgacggcgt
     1561 cgcgaccggt tcgaccgctc gagctgcctg tgaggtggcc agggcccagg gtgcgtcgcg
     1621 ggtgatcctc gcggtgccgg tggcgccggc cgattggact cgacgcctcg ccagcgccgc
     1681 cgatgagctc gtgtgcgtgg cgaccccgtc cccgttcttc gggatcggac agttctacga
     1741 cgacttcagc cagaccaccg acgacgaggt ggtccgccac ctgcgggccg ctgctgcacc
     1801 ggcgactccg acctctccgg agcccgggcg tcccgttgcc ggtgacgacc ccgcgatccg
     1861 cgacgaagag gtcgaagtgg aggccggacc attccacctc ggcggtcacc tcacggtgcc
     1921 cgagacggca cacgccgtgg tcgtcttcgc ccacggcagc ggcagcagcc gccacagccc
     1981 tcgcaaccgc tacgtggcaa cgatgttgaa cgacgcgggg ctggccacgt tgctgttcga
     2041 cctgctgaca ccggccgagg aatccgaccg ggcgaacgtg ttcgacatcg agttgctcgg
     2101 tcagcgactg gccgccgtca cggcctgggt gcaccaacag cccgatgtgg ccgggctgcc
     2161 ggtcggctac ttcggcgcca gcaccggagc cgccgcggca ctcgcggccg ctgccgcacc
     2221 cgacgttgac gtcttcgccg tcgtgtcgcg gggcggacgc ccggatctcg ccgaccggtt
     2281 gctcggctcc gtgcgtgcac cgacgctgct catcgtcggt ggtcgcgacg atgtggtcct
     2341 cggcctcaac cgtgaggcac aggtacggct gcgctgcccg agccagctca ccgtgatccc
     2401 cggggccacc cacctcttcg aggaaccagg cacgctccag gcggccgcgg cggcggcgcg
     2461 tgactggttc ctccaccacg ccccccgggc acgcacggtg gccagctgac ctcgtcgacg
     2521 tcccacggga ccttcgaccc tgttggcgag tgggtatggg gtgcagagtg ctggtcagga
     2581 gttgcgagat gtgagaggga gccctccggg gctgagaggc tggatgagtc cagtgacccc
     2641 cacgacctga tccggacaat gccggcgtag ggagatcatc ccgcaacccc acttcgcgtc
     2701 gaggggtggg gtggatggtg atgacggcag cgccgaaccg tgtggtgtgg gttgatcttt
     2761 cgaccaccga tctggagtcg gcgatccact tctacgagcg gatcttcggc tggcactacg
     2821 aggagcacga ctccggcatg cagaccaacg tggtggctcg ggtcgctggc ggtgaggtcg
     2881 gagggatgat ggcccagcct cccgagcagg ccgcctccgg ggtcccgtct gcctggacgg
     2941 tcttcgtggg ctgcgggcac ctcgagaacg cactgacctg ggcacgtgag ctaggtggat
     3001 cggtgctcca ggcgcccgtg tcgatcccag gcggggctcg cgtcgcggtg atcgccgatc
     3061 cgagcggcgc ggcgctggcc ttggcggagg gtgcgctggc cgtggggacg gccgtgcgag
     3121 atgagcccgg tgcgctggct tgggtcgagt gccagtcgcg cgacctcgcg aggagtcgag
     3181 ggttctacga ggagctcttc ggctggcagt ccgaggaggg caccgatggc tacgtggtgt
     3241 tcaccgccga tggcgagcgg gccggtgggc tcatggccat gcctcgcacc gtgcctgccg
     3301 aggtgcactc gcactggctg gtgtacttcg ccgccgaggt cgctgcgacc gcagacgcgg
     3361 tgaccgagct cggcgggacg gtcgttgtgc cggcgcacga gatcgacgaa gggcgcttcg
     3421 cggtgctcga ggacccgacc ggcgcggtct tcgcgatcta cgagcgctca ccgtgaccgc
     3481 catcgacagc ggcagatgaa gggggagacg tcgtgcacac cgagctgctg ttccgagatg
     3541 aggcgaggac caagctgctt gctggtgcga acgcggtggc cgacgctgtg cgcgccacgc
     3601 tcggtccaga ggcacgcagt gtcttgttgg aacgcaagtg gggcacaccg ctggtctgtg
     3661 atgacggtgt cacgatcacc aagcagatca agctgaagga ccaggtcgag aacctcggtg
     3721 cccagttgct gcgcaacgcc gcatcggaga cgggtgatgc ggtcggagac ggcacgacga
     3781 cgtcgacgct gctcgcccac gcgatcttca gcgagggggt gcgcaacgtg gtggtcggca
     3841 cgagcgccgt cgggatcaag cgcggcctgc agcgcggcct cgacgccgcc atcggctccc
     3901 ttcacgagct gtcgcggccg gtgaaggacc ggaccgacac cgagcacgtg gccaccgtgt
     3961 cggcccacaa cgaccccgag atcggccgtc tggtcgccga tgccgtcgac caggtcgggc
     4021 cagagggcgt ggtcgaggtc gaggaggcca agggaaccga gacgatgctc gaggtcgtcg
     4081 agggcatgca gctcgacaag gggtacctgt cgccgtactt cgtcaccgac cccgaacgca
     4141 tggaagcggt cgtcgaccgg ccgctcgtgc tgctcgtcga ccgcaagatc accgcggtca
     4201 acgagcttct gcccgtgctc gaagaggtgg ccaagtccgg gcgggggatc ctcatcatcg
     4261 ccgagacggt cgaaggagag gccttggctg ccctggtggt caacaggctc cgtggctcgc
     4321 ttgccgcggc tgcggtgaag gcgcccgggt tcggtgagcg gcgcaaggca atgctcgagg
     4381 acatcggcat cctcaccggt acccgtcccg tctccgaaga gctcggccgc aagctggagg
     4441 cggtgacggt cgacgagctc ggcagtgccg agcgcgtggt gatcacgaag gacaccacga
     4501 cgatcatcgg tggaggcgga gaccccggcg cggtgaacgg acgctgcgat gagctgcgcc
     4561 gccagatcaa ggacacgacg tcggactacg accgcgagaa gctcgaggag cggctcgcca
     4621 agctgtccgg tggtgtcgcg ctcatccggg tcggcgcgat ctcggaagcc gagctgaagc
     4681 gccagaagga ggccttcgac gacgccatca gctccaccaa ggccgccgtg gccgagggca
     4741 tcgtgcctgg cggcggcgcc gccttgctgc gaaccatcga cgcggtggaa gccgaagagc
     4801 aacgctgcga aggtgtcgaa cgcgtcggcg ttcacatcct gcgcaacgcg ctcgagatac
     4861 cagcccgtca gatcgcgaag aacgccggca tcgacgatgg ccccgtcgtc gagaagatcc
     4921 gcaacggcac cgggttcttc ggcttcgacg cccgcaccaa ggacttcgcc gaactcgacg
     4981 agcgcggcat catcgatcca accaaggtcg tgcgcatcgc gctgcagaac gccgtcgccg
     5041 tcgccggtgt cctcctcctc gccgaggcca ccatggtcga ggtcgaggag gaagcgccgc
     5101 cgcagctgcc cgagttcggc tgagggagac cgggtgtcac caggcgacca cgaggggccg
     5161 tggtcaccca caacgaccgt cgtaccgagg ggcgccccgt tggcggtcga acgtcgtctg
     5221 ccagccactg cacgtcctgt tctggaggtc gggtgagctg gattcctgca ccgacacgat
     5281 gccgccggag tgctcatcgt ggccgatgat gctgaacctc ggtcagtagg gcttcgggtc
     5341 gggttcccgg ctctgcacgt ggttggagac gtcgacgatg cccggtatcc gccagatcat
     5401 cgacgtcagc accgcaacgt cttgggggaa gagcgcggtg ccggtgagcg tgacgacgcc
     5461 gtcttcgacg gcgacttcga catcgaggtg ctcgggagcg cgccgcggat cgacgagcgc
     5521 agcctcgatc ccgtgtcgga tctcttcgtc gctgcggtgg aggatttgga gcatgtcccg
     5581 gcgagacacg acacccagga cgtggccggt ctcgtcgacg acgggcatcc gcttcactcc
     5641 ggcctccacg aggcggcggg ccgcgacccg gatctgaacg tcgggcccga cggtcgtcac
     5701 gtcgcgggtc atgacgtggg ctgctgtccg gccccttgcc ttgatggccc accgtgtctc
     5761 acctccggcc accaggtcgg ccaacacctg gatgagccgg cgcttgtggc ccccgtaggc
     5821 ctccttggga acgaggtcgg cctcggtcac gatgccgacc atcaccccgc tggtgtcgac
     5881 cacgggcagg ccgctcaccc cgtggctcag gagccggtcc acgagctccg gaaacggcgt
     5941 ctcgggagag accgtgatcg ctggcgtcgt catgacatca cgcacgcgca tctcgcacct
     6001 cacttctctt cgagagcatg tcgcgttcgg tctgcggcgg aacagaggcg ttcgtcccga
     6061 cgacagacag gaggtgcgct cgcccaggag atcgagtctg gcgtgaacgg cttcagggcc
     6121 gtgacgaacg cccaatcgtg ggcgaggggg ccgagtcgct gtccggttcg cgcaccgggt
     6181 cgatctcgtt gtgctcgaga cgagcgggat cggcaccagg acgtacgcca ggtcacggga
     6241 tacccgagcc catcgatgcc atccaccggc gctgccgaac cggtccgatc accgtcagcg
     6301 cgggtgaagc tgaatggcgg cgaggtggct caggcctggt gttggggtag ctggacccgg
     6361 ggtgggtccg tttcccgcgt cagctcctcc gccgtgtgta ccgatggagc gaaggccatg
     6421 tgatccgatt gggtcagcgt cggccagctc gactgcaccg gtgcgtcgtg gtgccggtgc
     6481 caccttttgg ccatgtccag catcgcttcc ctccgcctct acgcgtccga tgtcgactgc
     6541 gagcatccat cggctggcca tcttcgaaca gggtccttcg gcccgagact ctggtcggcc
     6601 cgtcggcgcg gtgagttcga gctctgggag catgcccacc gggcgcgggc ggcgacatct
     6661 gaagcgtgag cgtcggaccg cggtcgccgt caggagacaa cagacggtgt tcgcgttcgc
     6721 gccgacgggc atgcgtgtgg gcggctggtc ggcggtgacg gggccggacc ccgagcatca
     6781 ccgcgtcggg gaccttcggc tctggtcgtt ggtcgccggt cgggcatccg atggtgtgat
     6841 gagccccgag ggcctgcaca tcgggttgat cgccccaccg tgggttgcag taccgccgcc
     6901 acgctacggc ggcaccgagg tcgtgatcga ccatctggcc cgcgggctgg tcgcggctgg
     6961 gcatcgggtc agcctgttca ccaccgggga ctcgacctgt ccggtggaga agcactgggt
     7021 ggtcccggaa gccgtgggca cgaccggttc gctcaccgtc gagctcggcc atgtccaggc
     7081 tgcgtacgag cagctcgctg gttgcgacgt gatccacgat cacacgctgc tcgggccgct
     7141 gtgggcggtc gtcgacggcg tccagatccc gatggtgacc accaaccacg gcgagttcac
     7201 cccggagctg acggcgctgt accgcagcgt cggtcgacat gtggcggtgg tggcgatctc
     7261 ccaccaccag gccagcaccg cccccaccgt tcccgtgagc caggtcatcc accacggcat
     7321 cgacgtggac acgttccccg tcgggcgcgg cgacggtgga tacgtggtgt tcctgggacg
     7381 catgcacccc gacaagggcg tgcaccgggc catcgccgtc gcccggaccg ccggcaagcg
     7441 gctgctgatc gcagcgaaga tgtgggaacc cgccgaacat cggtacttcg ccgaacaggt
     7501 cgagccgctg ctcggcccgg atgtcgtgta cctcggacag gtcggcggag cggacaagca
     7561 cgacctgctc gccggcgcag aggcgctcgt caacccgatc cgctggcccg aacccttcgg
     7621 gctggtcatg atcgaagcgc tcgccgccgg cacccctgtc cttgcgttcc ccgagggcgc
     7681 tgccccggag atcgtcgatc acggacgcac cggcttcctg tgccgaaacg aggcggacat
     7741 ggccgctcgg ctcgtcacgg tcggtgccat cgatcgcgcc gcctgccgcc atgccgcgga
     7801 gtctcgcttc tccacccggc ggatggtcga cgaccacatc gcgctgtacg agcgcatcac
     7861 cgctgcccac ccacccgtcg tcgacctccg agacgcgagc catcgcggca cggcgagcgg
     7921 cccacgactc cgcaacctgg cttgaccaca acccaccaca gaccagacag caaggggagc
     7981 gacatgacac tgacgcgacg cacacgaagc gaaccggacg tgccggcatg gttcggcaca
     8041 cgacgcctgt tcgactggcc cgagtcctgg accgagctca tgggagctga gccccagctc
     8101 aaggtcgagg agttccggga ggacggacag ctcgtcgtcc gcgccgagat gcccggcatc
     8161 gatcccgaca aggacgtgga gatcacggtg gccggcgaca tccttcacct acgggcggag
     8221 cggcgatccg agaccaagac cgaaggcgcg tcgggctacc gctcggagtt ccactacgga
     8281 tccttctccc ggtcgctccg gctgcctgcc ggcgcaaccg aggccgacat caaggccacc
     8341 tataccgacg gcatcctcga ggtccgcatc cccatcgata ccgcaaccgc tgaggccaag
     8401 aagatccccg tctcccacgg ctgatcccat gatcccgtgg tgaacaggcg ggagtttgcc
     8461 agggagcggc agcggtgttt cgatcctggg ccctctcccg ccccgccggg gacggcctgc
     8521 gacttccggc gccgacgcac ggtcgaacca acaccactgt gcgccggttc gccctctcag
     8581 ttgccgtcct ggctcttccg gggagcaggt cggttgggcc tgtctggagg cgactgatct
     8641 cgcgttcgtg ctcccgtcga gccgggtgtt cgggacgtgg tgacaatcgc ctctggtggg
     8701 gccggggcat ctggcccatc gttcgagttg ggcgcacgac cggttcgggc gctgcgcccg
     8761 gttcccacga cgacgaagac ggacggtgca tgatgggcga gaagggcgat caggtgaagg
     8821 gtcgggccaa agaggccgcc ggagtgctga gcggcgacgc ggatctcgag tccgaaggca
     8881 aggccgatcg gcgcgcaggt gaggccaagg agaagttcgg ccacgcgaag gacaaggtcg
     8941 aagcggcggt cgagaaggtc gaggacaagg cgggcgaggt catcgacaag gcgaaggacg
     9001 ctctgcatcg gaagcgcacg tgatcgtagg aagaccccgg cagaccgggt gaccttcgcc
     9061 tcttccgacg ccgccaccgc cgaggcaacg aagatctccg tctcccacgg ctgatcggct
     9121 ccaccgacgt ctcacgtccc cggtggggcg accgctccct gcggcaccag cagcaccggt
     9181 cggtggctgc gttcgagcgc ggccctgacg accccggcgt gcccgggtga caggtcctgg
     9241 gcccaggtga gcgcgacggc gtcgggacgc tcggcgtcga ctgcctccat cacggtctcc
     9301 ggctccgctc cgaccaggag gcgcacctcg gtcggtgcct cggtgcacca ccgctcggcg
     9361 aagctggcgg cgagaccctc ctcggcgtga agcgagtgat cggagaacct cggcacgtcg
     9421 ccgggagcga agacgtgcac ggcgaggagc gcgaccccgg cgtcggccag ctgccgcagg
     9481 attgccgcga tcgggctggt cgtcgccgcc gagccttcca gagggacgag gaccttgcgg
     9541 acccgggtct ccgttgtcga gacccactcg ggaggtacga ccaggaccgg gaccccggcg
     9601 tgctcgacca gcgcgatcgc gatgtggccg gcaggtcgcg cgccgtcgac tgctcgccgc
     9661 gacccgatca ccacgaggcc cacggacgga tccgacgcga ggtcgaggag gaccctgccc
     9721 gggtcgccgt cgagcacctg cacgtcgagc ccggcccgcg ccgcactcgc caggacgtcg
     9781 ggtgggccgt cctcgttcac gtgcacggcg cgcagctccg ccgtgagcaa ggagctccag
     9841 atgtgggccg cccgcagcac gcactcggcg gtcgggctgt cgtcgatggc ggcgaggacc
     9901 gtgatcatga ggtcatctct gctctctggt ctcggaggtg atcgtggcgg atcgcctggc
     9961 tgacgggtgc ggtgagctgg acgccctgga cgcggaccgg gatgggctcc tcggcctcca
    10021 ccgacacggt ccggtggtcc acgtggagtt ccaccagcac cccctggtgc cggaaccgga
    10081 cccgcaccga ctcccagctc gtcggcaggt gcgggtcgac ctgcagcgtg cgtcctccgg
    10141 ggatcacgcc cacgaagccg aacacgacgg cctgccagat cccacccatc gtcgccaggt
    10201 ggaggcccga gctggtgctc ccggtgaggt catcgaggtc gatccgggac gccaggcgga
    10261 acagctcgag cgcctcgtcg gcccgcccgg cgcgcgcgag caccgccgcg cagacggctg
    10321 gcgacagcga gctgccgtgg cagatcagcg ggagctcgcc gtcgaggtcc gcctgcagct
    10381 cggcgggaga gaacgcggtc gggagcaggt ggtgcgccat gaggacgtct ggttgcttca
    10441 ggaccttgct ccgcacggtg cgcgctcgtc ccaggacgat gtcggcggcg accggcgtcg
    10501 gtcccaggtc ggccaggtcc accgcctcga gctggtcgaa cccggcgaac tgctcgtggc
    10561 gaccgcccgc ccgccgtccg acgaccagcg cggcagcgcg gcgacgcagc gcggacgcgc
    10621 gctcgtcgtc gccctcggcg gccaccaggt gggcggcctg cccgaggtgc caacggacca
    10681 tgaggttcgt gtaggcgtcg tcgtcgacgc actcgtggta ctcgtcgggt ccggtcacgt
    10741 cgtacacgtg cgccaggccc cactcgtcga gctgcgaccg gtccatccag tagctggcgg
    10801 tgtcgacgat caggtcacgg ccgccggagg ccaggaacga cggatcaccg gtccagtcga
    10861 ggtagtgcac cgccgcccac gccacgtcgg acgtgatgtg ctcggcgagc tggccggtgg
    10921 tgatcggcac cacctctccg tgcatgtccc gcccgaagcg tggcgtgacg tcctcgccgg
    10981 aatccgccga ctcccacggg aagcgggcac cggcgagccc gccggtccgg gcacgcgcgc
    11041 gggcggcagg aaggcgacgg atccggtact cgaggatcga tcgtgccgcc gccggcgcca
    11101 cggcggccag ggccggcagg atgtacacgt ccgtgtccca gaagacgtga ccggcgtagg
    11161 cgagaccggt gagaccccga gcgcccaccg gcgattcgtc gccgggagca gcgcaggaga
    11221 ggaggtggaa gagggcgaag cggatcgcca gctgcgcttc gggatccccg acgatctcga
    11281 tgtccgcatc ttcccagcgc gaggtccatg cggctcggtg ctccgcgacg agcccgtcga
    11341 cgccgagggc gaccgcctcg tccagcgcgt cccgggcggt gccctccgcc cccgggccgc
    11401 gcgccatcga cacgatgcgc tccatgcggt gcgggcgccg gacctgctgg ctggcggtgg
    11461 ccaccaccac ctcgcgaccg ccgcgcacca ccgcggtgtc cgtgccatcg cccccgctgc
    11521 tgatctcgca cgtgtggtcg gcgcagagcg cacgcggcac atccgggggc gcgagcggtg
    11581 ccggccaccc tccgccgacg tcgccggcag cccagaggac accgatggtc ggccgcacga
    11641 ccgacaccag gcgcgctgcg gtcgtgcggc cgcgccgatc gatgcgggag aggaggccgt
    11701 cgcgcaggtc gagggcccac gccgtgcccg gggggtcacc gccgatggcg gtccaggccg
    11761 gtccggggag cgggcgcacc atcccgtcgg ccgcgacccc gtacgccccg gcgacgaagg
    11821 tggctcgtga tccgaccggg tcggggccga cgtcgccgac cacgccgatg acgccgttgg
    11881 cgagcgcggc cagggtgccg tggtgcccgc ggtggtcgcc gacctgcagc gtccacgccg
    11941 atggccggcc gagcgtctcg ggtgacgggg gcccttcgtg ctgcggctcg gcaccgtcga
    12001 tcaggtctgg gaccacgggg acggctccgg gtcaggccga gtgcgcacga tcaggtgcac
    12061 cggagcctcc tggtcgacgt gacgggggcg gatcagccgg ccgaggtcgc ccgatccgga
    12121 cggagcggct gcggaccggg gtcgcggggt ccagcatccg gcggtacagc tccaggtggc
    12181 gcgccaccat gcgctcggcc gagaagcggg tgacggctgc ccgtcggcag gcccaccgct
    12241 cgatctcctc gacccgtccg atcgccgcga ccatctcgga ctcgtcggtg cagacgaacc
    12301 ccgtgcgccc gtgctcgacg atctcgggcg ctgcgccgcc ggccagggtg atcacggggg
    12361 tgccggcgcc gaacgcctcg atcatcacga gcccgaacgg ctcgggccag cggatggggt
    12421 tgaggagggc gtcggcaccg gcgagcagct cgtgcttggc cggtccgctc acgtgcccga
    12481 tgtactcggc gtccgggccg agcaggggcg ccacctcggc atcgaagtac gcccgctcgg
    12541 cgggctccca catcttggcg gcgatgacga gtcgtcggcc tgcggcgcgt gcgatccgga
    12601 tcgcctccac cactcccttg tccgggttca tccggccgag gaacgccagg tagccaccgt
    12661 cgccggtccg ccgccagtcg ctcggcgggt cgatgccgtg gtggatgacc gcccgcacgt
    12721 cgacctccgg cgcggtcgac cgctggtggg ccgagatcgc gacgacggag acgcgtccgg
    12781 ccaccgaccc gtacagcgcc cgggacgccg gcgtgaacgt cccgtgcacg gtcgccgcga
    12841 ccggcgccga ccgtggttcg ttcaccgccc agagggggcc gaggaaggtg tggtcgtgga
    12901 tcaggtcgca gtcgcgcagg gagtcgtagg cgtcggcgac gtggtgcacc tcgacgtcgg
    12961 tgctcgcttc ggtcccggct gctcggtcga gcacgccccg ccgctcgacc ggacaggtcg
    13021 agtcgcccgt cgtccacagc accacgtggt ggccggcacg ctccagtccc cgagccagct
    13081 cgtcgacgac gagctccgtg ccgccgtagc cgcggggcgg gaccggcacc cacgggggtg
    13141 cgatcatgcc gatgcggagc cggtcgggca tggctccagg ttcctcctgc gacacgtccg
    13201 ccaccagggc cgttcgtcct gcaaccgtcg cctgcggtgc ggcagccgcg gtcgagcgag
    13261 ccgaggggac gatcgctcac gttgcgcggt cgccggtcga cggggagcag cggttgtcgg
    13321 gaccaggacc ttcggccctt ccggggaccg gcgggcgggg gtctgatgga acccaccgga
    13381 ccgatccgag gagcatcaga tgcaggcgac caggggcgac aggatcatca tccgcggtca
    13441 tcgggtcggt gagccggacc gggacggcga ggtcctggac gtgcgcggtg aggatggagg
    13501 cccgccgtac gtggtgcgct gggcggactc cggccacgag accctcttct tcccgggctc
    13561 ggacgcgacg gtccagcact tccggtctgt gacaccggcg gagacgggac catgaccgac
    13621 ccgaccacgg cgatctcctg gttcgacgag ctgtcccgca ccgacacggc gacggcaggc
    13681 gggaaggggg cgaacctcgg tgagctggcg agtgccggcc tcccggtccc ggatggcttc
    13741 gtcgtcaacg ctcgcggcta cctgggtgcg atggacgaag gcggggtccg ggacgagctc
    13801 caggccgcct tcgccacggc ctgcggcgag gcgtcgacgc cgaccgccct ggcggacgcc
    13861 gcggagcgcc tgcagtcgct ggtgcgcaag gccggcgtgc cggccgggct gcgatcggag
    13921 gtcctgcagg cgtaccaccg cctgggtgac cgcgtcccgg tcgccgtccg gtcctcggcg
    13981 acggccgagg acacggccgg cacgtcgttc gcaggcatgc acgagacgtt caccgacgtg
    14041 gtcggggacg acgcggtcct cgctcggctc gtcgactgct gggtgtcgtt gttcggccag
    14101 cgcgccatct cgtaccgggc gagccagcgc atgaccgacg agccggtcat cgccgtcgtc
    14161 gtgcagcgcc tcgtcgccgc agatcgggcg ggcgtgctct tcacggccga tccctccacc
    14221 ggcgaccgca gccgcctcgt catcgaaggg gcgttcggtc tgggcgaggt cgtcgtgtcg
    14281 ggggcggtcg agccggacac gtacgtggtc ggcaaggacg gtccccggat ccagctggcg
    14341 aggatcggcc acaagaccca ccgcctcgtc gccgagccag atggatccgt cacccgggtc
    14401 gacctcgacg acacggaggc cgacgcgcgg gtcctggtcg atgacgaggt cctcgacctc
    14461 gcgcggctcg ggctgcaggt cgaggaccac tacgggggag agccccagga cgtggagtgg
    14521 gcgatcgccg ccgggcgcct gtacctcctc caggcccgtc ccatcaccac gctggcggca
    14581 cccgaggcgg cgtcgccgcc ggaggccgag acggctgatg ccggtgtgcc cttggtccgc
    14641 gggttgtcgg cctcacccgg ggtggtggcc ggatcggtgc gggtgctggc ctcgccggcg
    14701 gaggggcacg tcctcaagct gggtgaggtg ctcgtggcgc ccatgacgaa ccccgactgg
    14761 gtcccgacca tccgacgtgc ggctgccgtc gtcaccgatg gcggcggcat gacctgtcac
    14821 gccgcgatcg tctcccgtga gctcggcgtg ccctgcgtgg tcggtgcccg cgacgccacc
    14881 acccggctgc gggacggcga catcgtgacg gtggacggcg gtgccggcga ggtctacgaa
    14941 ggcgatcggg tcggcgctcg gacggtgccc gccaccgcgt cggctccgtc ggtggtgccg
    15001 tccggcgagg cgctggcgac gcgcatctac gtgaacctgg ccatcgcgga ccacgccgag
    15061 gccgtcgcag cccagcccgt ggacggggtc gggctgctgc gggcggagtt catgatcacc
    15121 gatgcactcg gtggggtcca cccgcgagcg ctgctcgagc ggggcggtcg cgccgagttc
    15181 ctcgaccgga tgtcggcgtc gctgctccgc atcacgcgtg cgttcgcgcc acggccggtg
    15241 atctaccgga cgatcgactt ccggtcgaac gagttccgag ggctggaggg cggcgcggcc
    15301 ttcgagccgg tcgaggagaa ccccatgatc ggctaccggg gctgcttccg ctacgtgcgc
    15361 gagcccgacc tgttcgcgct ggagctggag ctgctcggcc gggtgcgcga ggagacgccg
    15421 aacctccacc tcatgatccc cttcgtccgc accacgtggg agctcgaggc ctgcctggag
    15481 gcgatcgacg cgagcgtgct cggtcgccag cgagggctcc accgatgggt gatggccgag
    15541 gtcccctcgg tcgcctaccg gatcccggag tacgcggcga tgggcatcga cggggtgtcg
    15601 atcggctcga acgacctcac ccagctcatg ctcggcgtcg atcgcgactc ggcggtctgc
    15661 gcggagctgt tcgacgagtc ggatgcggcc gtcctcgacg cgatcggccg catcatcggt
    15721 gcctgccagg ctgctggcat cacctcgtcg ctgtgcggtc aggcgccgtc caacaagccg
    15781 gccttcgccg agtacctggt gcgcctcggc atcgactcca tctcggtcat ccccgacgcg
    15841 gtcgatgcgg cgcggcgggt cgtcgcctct gcggagcggc gcctgctgct cgatgcggcg
    15901 cgcgaggccg gtcggcgccc tgcgccgcga tgacccccgc ccgaccacca ccgcaggccg
    15961 ggggcaccga gccgtacgcg atggcggtcg agacccacac cggcgcggtg ctgctcgtcg
    16021 gtgatctcgt ctacaaggtg aagaagccgg tgtccgtcgg gttcctggac tggaccgacc
    16081 gggccgaccg ggtgcgcgcg atcgaggacg agctggtcct gaaccgccgc ctcgcaccgg
    16141 atgtgtacct cgggaccggg ctgctccccg tcccgggggg cgaggacgag ccggtgctgg
    16201 tgatgcgccg cctgcccgcg gaccgccgcc tctcgacgct ggtggagcag ggtcgagacg
    16261 tcgacgacgc cctccgagcg ctggcgcacc agctcgcggc gttccacctc cgcggcgcga
    16321 ccacgacggc cgctgcggcg gcagccggac ctgcggagac gctgcgccgc tgggaggtca
    16381 accacgagcg catgcggccg gcgttcgggg agtggatcga cggtgaggcg gccgagctcc
    16441 tgctcgagcg agcccgcgca tacctccgcg ggcgggccac gctcctggag gagcgcgtgt
    16501 cccgtggctg ggcgcgcgac ggccacgggg atctcctggc cgaggacgtc ttctgcctcg
    16561 acgacggacc gcgggtgctg gactgcctcg acttcgacga gcggctccgg gtcggtgacg
    16621 tgctcgccga catggcgttc ctggccatgg acctcgagcg cctcggccga gcggacctcg
    16681 gttggcgcgt gctcgagctg caccgggaga tgaccggcga cagctggccc ccgtcgctcg
    16741 cgcaccacca cgtggcctac cgcgcccagg tccgcgccct cgtgtcctgc gtgcgcgctg
    16801 cgcaggggga cgctgcggcg ggtgtgcagg ccgcaccgct gctcgacatc gccggccggc
    16861 acgccgccgc agcccgagtc cggatggtgc tcgtgggggg cgcaccggcg acgggcaagt
    16921 ccaccctcgc ggcagggctg ggtgatcgcc tgggtgcgac ggtgctgcgc tccgacgaga
    16981 tccgcaagga gctggccggt cgatcggtgc gtgacgactg cggtgccgag ctgggggcgg
    17041 gcctctacag cgaggcgtgg accgaccgca cgtacggcga gctcctccgc cgcgccggtc
    17101 ggctgctcgc ccgaggcgag tgcgtggtgc tcgatgcgac ctgggccgag caccagcgtc
    17161 gccgagcggc acgcgtcgtc gccgaagcca cgagcaccga cctcaccgag ctgcgctgcg
    17221 tggtcgatgt cgagacggct cgtcaccggg ccggcgcccg cgcccgagcg ggtatggacg
    17281 tctcggatgc ggacgccgcc gtcgcctccg ccctggctgc gtccttcgat ccgtggccgg
    17341 atgctccaga gatcagcacg gcgtcgaccc cggagacggc gctcgacgcc gcccttgtgg
    17401 tcctcgagcg caggtagcgc tcccctcagt cggtcgctgg accttcctgg ccctgctcca
    17461 ggtagaggta gaagacggtg cagccctcgt ccccgaccgc ttcgaagtcg tgctcgaccc
    17521 cggccgggat gtgcgcgtag gaaccagccg ggagatcctc tccgagcacc cgcgtggacc
    17581 ccgtcaccac ccacacgtgg tggtggtggc ggcgatgggt gtgctcgccg aggtggtgtc
    17641 cggcgtcgat gtgcatgaca ccggcgctcg agcgcccgtc ggtccacgtc gtccggtgga
    17701 ccgcaccgcc cccgaccccc agcggctccg cgtcgagggc ggtcaaccgg cttgccgaga
    17761 cgagggccac gcctggttcg gatctcgctc ccatgcaggc agcgtcgggc atcgcgccgc
    17821 tgcacgccag ggcccaaagg cactccggat gggatccagt cctcggctac gcggcgccga
    17881 gctgtgcctc cgcgcgcaag agggtctgct cggcacgttc cccgaactcg gagggtcgat
    17941 ccgactgcgc cgcccgcacc cggacggtca gccggccgag gacgcgaggg tcccggatga
    18001 ggcggacgag ctgcgccgcc gcccgaccgg ccgaccagtc gtcggccatg accagcttga
    18061 cgagcacgag catcccccga tcggtctccg tccaggtccg tcgccgtctg ctggcctcga
    18121 tctgcatcga tctccacctc cgctgagccg tctccgagcg aaggtagggc ccggcgtggc
    18181 gacgacggac ccccaatccg tgggtgctgg cgacgtcggc cgggtcatac cgtcgggatc
    18241 catgacctcc gtccccacgg gcattgtgcg cggacccgac caaaccgacg agtcttcgct
    18301 cgagcgcctg cccctcgccg tgctggagct ggacggtgac ctgcgcgcgg tcgacgtgaa
    18361 ccaggggtgg gtccagctgt gcggccgtag ccagcacgac tcgcgcggct cgctctggtg
    18421 gcagagcgtc gacccacgtg accgcgccgc ccttcagcgc gccctgtcgt caccggcgcc
    18481 gtcggatgac gtgcgagcgg agcaccgcgt ggtcggggcg ggcgaccccc gttggacgcg
    18541 gtggtggtgg cggcacgacg gggaccgcac gtgggcgtgc gtcgcggatg tcgacgccga
    18601 ctggtcccgc acgcgcgacc tgtggcgacg ggccacccac gacccgctga ccgggttggc
    18661 gaaccgcacc gagttcatga cgctgctcga acgggcgctc gatcgagcga tcgaccaccc
    18721 ggccctggcc gtggtcttcg tcgacctcga ccacttcaag ggcgtgaacg actcggctgg
    18781 tcaccgggcc ggggacgcgg tgctcgcagc cgtcgcacac gcgatcgtcg aggccgtgcg
    18841 cccgttcgac gtcgcggggc ggttgggagg cgatgagttc gccgtgctct gccccgggct
    18901 gacgtccgag gacgagacca ccgcgctcgc cacccgcatc cgggacgcgg tcgccgcctc
    18961 ggaggcggtg accggcatcc ccatctcggg cagtgcaggg gtggcggtga gcgtcgccgg
    19021 tgacggctct gccgacagcc tggtggcgcg ggcggaccag gcgatgtacc gcgacaagtc
    19081 ggctcgtcgt ccgccctccc cctccggtgg cggtcgaccc gcaccgctgt ccgctgacgt
    19141 cggcgaacag cacgtcgcag cggtcctcgt gccgatgctg ttccggctcg gtctgtccct
    19201 ccacgcggtg gcggacgcag ccgacgacct gaccgcgtcc cgggtgcgag acgtcgtcga
    19261 ggagctcgac gacgtcatcg cctcattgcg ccacgcggcc ttcgcgcacc tccgggacac
    19321 cgccgacgct gcatcggtcc gggttgcgtt ccgccgagtc gaatcggcca tcgctggtgc
    19381 ggagcacgcg gtgcgtcagg agtgggcgaa gacagccacg tccgcctcgg atgatccggg
    19441 gctcgccctg cggctggtcc gcagctcacg gctgctccgg gccgcggagc gagcgctcga
    19501 accagacgcg ctgggttgat cggtcaggcg ggaacgcctc ggtcgcgccg tgggcgacgt
    19561 cccgggcgag cgcgagcagc tccgagtagg tggtcggctt ctcgcggtac gccgtcgccc
    19621 cgagctgctc cgccagcgag cggtctgctg cggcacgcga tgcgctcagg acgaccacga
    19681 ccaggtcctg ggcgccaggc aggccccgga gcgcccgcag gacgtcgacc ccatccagat
    19741 ccgggaggtt caggtcgagc agcactgcgt cgggccacgg ctccacccgg aaccgggcga
    19801 gtgcgtcgcg gccggtgctg caccgctgca ccgacacctc gggcgcggcg tcggcgaacg
    19861 cctcggaggt gagcagggcc caggcagggt cgtcctcgac gagcagcacc tctcgcgacc
    19921 gcgccatgtc agcgaggctc cgaggccgac gtcgacccgt ccggatggac ggccggcaac
    19981 gtgaacgtga acgctgcgcc gccatccggc gcgtcctcga ccttgatccg cccaccgtgg
    20041 agctgcacga tccggcgggc gatcgacagt ccgatgccga gacctggtct gttgccatcg
    20101 aggcgtcgga acgcctggaa gatgcgccgg cgctgctcgg gcgggacgcc gctgccgttg
    20161 tccgacaccg tgatgcgcca ccgggcgccg tctcggacgc tctcgatccc aatgcgcagc
    20221 ggatcgtccg cgtcggcgtg gcggacggcg ttggccacca ggttctgcat gagctccacg
    20281 agcaggtcgc ggtgtccgac gaccgtcggg aggggcccca cgaccacctc ggcgtcgcac
    20341 gaccgcaggg cggactcggt gttggccagt gcgtcgcgga cgacttggtc gagcgggacg
    20401 ggagctggtg gctcgtgggt ctccatgctc gacatgtgga ggagcgcgtc gagcatggcg
    20461 cgcatccgtt gcgcacctcc ggcgatcgtg cggaggtggg tcgccgctgc atcgtcgacc
    20521 gtcgagccga cctggtactt ctggacgagg aggtccgcgt acccgccgat caccgtcagg
    20581 ggctcggaga ggtcgtgcgc agcgatcgtc gcgatcgcct ggagatcctg gttcgagcgc
    20641 tcgagctgtc gggaacgggc gcggagctgc cgtcgcgcct gccgctgggc ggtgacgtcc
    20701 cggaccacga cgacgcgagg tgacgccggg tcctccggtc ggagcggacg ggcagcgagc
    20761 tcgacgacca cccgtgaccc gtcgtttcgg cggacctggt gctcggtccg gtcgccgatc
    20821 gacgccggat cgtggatcgc agggagcagc tgactgatgt gctcaccccc gagcgccgct
    20881 gcgtcggtgc ccagcagatc gcgggcacga ggagaggcgg acatgatcgt gccggcggca
    20941 tcgaccacca gcaacaggtc gggcgcggcg tcgatcgtcg cccggagacg ttcctcggac
    21001 tcgatgagcg tctgcgtcgc ccgcttcgcc gtgccggccc accgcgtgct cccccgggcc
    21061 accagcccga ccagcacggc caggagcgcc gtcagcgacg ttccggccgc gatcgctgcg
    21121 gcagatcggt ccagctgcgg ttggacgatc gacgatggcg cggtcgcctc cagcacccaa
    21181 ccgatctcgg cgttcgggga gcggagcggc tgcagcgtcc tcgccgtcgc tccgtcaggc
    21241 accggtccgg ccgcggcgat cgcaggccgg tctggttcgc cgtcgcgcaa gaccacgcgc
    21301 acgccggtcg ggagtcgcag ctcatcgagg agctcctgcg cgcggaacgg gaggttcgac
    21361 cagccgacga ccgcttggcg gcgctgctcg acgctgccgg tcggtgcgcc gggtgcgaac
    21421 accggcgcgt aggccacgta gccgaccgga cgctctgccg gcgcgacgtc gcggtcgacg
    21481 gcgagcacca gctgctccgt cacggccgtc cggccgccgt cggtggcccg ctgcagcgcc
    21541 cgggcggctt cgtcgacgga ggtcacgtcc acgccccagg acggacgcag ctcggcggtg
    21601 ggctcgttgt agagcaggat cgcgtgctcc ggcccggtgc tgaggtcctg gagctcgaag
    21661 tcgggggcac cgtcggcgcg cgcaccggcc acgaagctgt ccaggtcggt ggcggcgaca
    21721 cgccggacgt agccgacgcc gaggagcgtc gggagctggt ctgcgatgcc gccggcgtag
    21781 cgcgcgaacg cggcccgatc gaggtccggg tccacggaca gggccgccgc cgtcgccgcc
    21841 gccccgccga tctgggtctc gaggagcgcc gagagctcgt ccgccaacgc atcgccgatc
    21901 aggtcggccc gctggcgcag gtcgcgctcg cggacccggt cctcgtagtc ggcggccagc
    21961 gcggtggcga ccgctccggc cgccaccacg gcgaggggtg cgaggacggc gaggctcctc
    22021 gtccagcgcg ggccgtcccg cttccgctcc cgggaggtca tcgcagcaac agcccggctt
    22081 ggtgcgcgac ggccaccgct tccagctgcg agtgcgcgcc gagcttggtc aggacgttgc
    22141 ggacgtagcc gcggcacgtg tgcacggtga tgcccagctg gcgagcgatg ttggttgcgt
    22201 cgatgcccgc agccaggagc gcgagcacct ccttctcgcg caacgtgaga tccgggcgcc
    22261 aggtgcgccc gctgatggaa ccctcgtccc cgacgcgtgc tcgcagcgac cgcaggatcg
    22321 actcgtcgag cgtgagccct cgggcatcca tgcggatcat gcggatgagc tgatcgatcg
    22381 gggtgtcctt ggcgatgaac ccatcggccc ctgcggctgc ggcccggagg tacacgtcca
    22441 ggtcggcgag cgcggtgagg atcacgaccc gcacgtccgg atgtcgggac ttgacccgac
    22501 gcgtcccgtc gatgccgtcc acgtcgggca ggtcgaagtc catgagcacc acgtcgaccg
    22561 ctgtgtggtc caggatctcg agtgcctccg caaccgaggt cgcaccccct gcgcagcgga
    22621 ggtcgccgac gggatccgag gtgatcgcga tccgcagggc gtcgaggaac gtgcgctggt
    22681 cgtcgacgca gagcatggcc agcggctcac ccatcgtgcc cacccggtgc gtcggtgccg
    22741 gtgccgagcg cgtcgacgac atcccgcagg gcggccacgt tcgcttcgat gtcgaggagg
    22801 agctggagct gcatcgccgg ctcgagctcg ccacctcgga cgcgcagggt ggatgcggcg
    22861 acaccgatcg tggcgagggc accgcgcagg cggtgcgaca ggacgtcggt cgtgctcgcc
    22921 gcggatccgg cgcgttccgc tcgttcctgg tgcagcagca gggcggtgcc gatgacgtga
    22981 ccggcagcgc tcgcgaagac gtgcgcgacg gtcgaacgcg cgtcgatccc gctggcgcgc
    23041 aggtcgccga agtgtcgacc gtcggcctgc agcggtacca cgacgtcggc cgcgccggac
    23101 gatccgccgt tcggcgcgac ggcccgcagg tcgaaggcca cgcggtcacc cagcacggac
    23161 cgcacgatcg ggagcgcccg ccggaggatc tcctgggcgg tggctgcgtc gtcgagcccc
    23221 cggcacaggg cgacgacggc gccgagcacg tcgtcctcgg gcttggccgg ccgccggggt
    23281 gcctggtcgt tcgagtccac gccccgaggg tcccccggga tgcacggcag gggaagaggc
    23341 gttggtcatg gccgtcccgg ctacggcccc ggcggagcgt ggaccacctc ggacagcggc
    23401 ggacgggcgt ggcggacgac ccggaggtcg gcggcgccgt ccacgtggac gtgcccgtcc
    23461 gacacggtca ctgccaggtc gtgcggaccg acgcggatgc cggagacggt gaggtcaccg
    23521 aagccgtcgg ggagctgggg gtcgagccag atcgtcccgt cggaggcggg ggggtcgaac
    23581 cggagcaggg tgcgcagcca gagcatcggc gatgccgccg cccacgcctg cggcgcgcac
    23641 gaggccgggt acgaggcggg gacggcgaac tcgtcccggg agaagccggt gaacagctcg
    23701 ggaagccgac cgtcgtgggc gcgcgccacg tcgagctggg cctcgatgat gcggtgcgca
    23761 gcgtcgacga agccgtaccg ggcgaggccg gccgcgcaga gggcgttgtc atggggccac
    23821 acggagccgt tgtggtagct caccgggttg taggccgctg ccgacgcgga cagcgtgcgg
    23881 acgccgaagc cggagaacat gtcggtcgag agcagccgat ctgcgacgac gccggcacgt
    23941 gcggggtcga tgatgccgct ccacaggcag tggccgatgt tggacgacag gacgtcgacg
    24001 ggtcgcttgt ccccgtccag ggccagcacg taccagccgt gctcgtcgga ccagaagtcc
    24061 tcgttgaacc ggcggcgcag ctcgctcgcc ttcgcggcgt agcgctccgc cgtggcggcg
    24121 tccccggcct cgcgcgccag gtgcacgcgc gcgacgtagg cggcgtagac gtagccctgc
    24181 acctcgcaca gcgcgatggg ggtcgtcgcg agctcgccgt cacggtggcg tacggcgtcc
    24241 caggagtcct tccagccctg gttggcaagg ccggcctccg tcgagcgctg gtactcgacg
    24301 tagccgtccc cgtcccggtc gccgtgctcg tcgatccagg ccagggcgcg gtcggcgtgg
    24361 ggcagcaggc gatccaccag ctcgtcccgc gcatcccacc ggcgcagctc cccgagcagc
    24421 atcacgaaca gcggggtcgc gtcgaccgag ccgtagtagc gctcgccacc accgagggcc
    24481 aggttcgtcg tggacccgaa gcgcatctcg tgcaggatct tcccgggctc ctcctcggtc
    24541 ttcggatcga cgtcccggcc ctggaagcgc gccagcgtct ccagcacccc gtgcgccaac
    24601 gacgggtcgg cgatcaaggt catccaggcg gtcagcaggg agtcgcgccc gaacacggtc
    24661 atgaaccacg gggcgccggc ggccaggatc gggagctcgg ggtgctcggc gtcgaagatg
    24721 cgcagggcgc ccaggtcctc ggccgagcgg gccaacgtct gggccagggg tgcgctggcg
    24781 gtctccaccg tcggcagggt ggcgtgccag gtggtcatgc gctgccgcgg gagcgactcg
    24841 tggtcctgct ggccgcacag gaacctcggc tcgacggcga tgccccggat cgtggctgcc
    24901 acctggatgc acacctcgaa ggaggcgccc ggctcgaggg tctgctccca ggcgatcgag
    24961 tccgacccgg tggtccccgg tggagaggcg ctgatgcgca cgtccttcga ccccgcctcc
    25021 gaccgctgct cgaagtgcag cgacccgctc tcctcctccg accggatctg gccgagcgcc
    25081 cgagcgcgac cctccttcac ggcgaacaga tcggcgaagt cggcgtcgac ggtgaggtgg
    25141 agggccatcg gcgcggatcg gaggccgtgg ttgcgcacga cgatgcgctc ccgcatgccc
    25201 tgccccacgt ggcggtgacg caggacgacg acgtcggcgt cggcgtggcc ctccgccggt
    25261 gcgctccgcc cgaggaaggt cgcgccgaag ggctcgtgca cgtcgacccc gagcggttcc
    25321 acccggtgcc cgttcagctg gagggaccag ctcgacacga tccgggtgtc caggaggaac
    25381 aacccgtggg ggaagtcggc ggtgatgtcg cccgcccgcc cggacacgca gaaggactgg
    25441 ccctccacga gcgtgacgac gcccacggtg gccgacgacg gcaccggcgt ggtcccggcg
    25501 aagggagagg gtgcgtcggc ggatgacggc tccgttccca tgtggatgac gctggcgcga
    25561 ccgacggcgt tgcgcaaggg ccaaaacgcc gtcgcgtccg accgacggtc gtcatggtgg
    25621 tccgtccgcg ccgtcgagct gggtactccg gcgctgcgcc ttgtcaacgg cgtcgacgg
//
LOCUS       DATISU010000026         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_746253, whole genome shotgun sequence.
ACCESSION   DATISU010000026 DATISU010000000
VERSION     DATISU010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746253"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VLR27_01330"
     CDS             complement(<1..602)
                     /locus_tag="VLR27_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HSP02111.1"
                     /translation="MARDLAIDLGTANTLVYAKGQGIVMNEPTVIALNKRNGDVLAMG
                     REAWQMIGRTPSYIIAVRPLRQGAITDFDITQRMIRLILQRAGVNRFNRPRVVICVPS
                     AITEVERRAVTEAARRAGAADAQLLEQPMAAAIGAELPIHEPVANMVIDVGGGTTETA
                     LISLGGIVALQAVRVGSFDIDAAIQSYVRREYGIAIGERTA"
     gene            complement(605..805)
                     /locus_tag="VLR27_01335"
     CDS             complement(605..805)
                     /locus_tag="VLR27_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02112.1"
                     /translation="MIPFLLVIVVIVVVGVSVLAYRGRPESSIESGIRSFRREMRALA
                     PPPEHRGGPDDQHPDAPHRDAQ"
     gene            complement(802..1308)
                     /locus_tag="VLR27_01340"
     CDS             complement(802..1308)
                     /locus_tag="VLR27_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HSP02113.1"
                     /translation="MIPSATLEPPARGHDWFALAEEPLPVQVATDWVVRPDCGGVVTF
                     VGTARDHSEGRPGVEVLEYEAYETEVVPRLARVAAEARARWATVGNIALLHRIGPLQV
                     TEAAVVVSVSAPHRDEAFAAARFCIDALKQSVPIWKKERWDGGESWGLEAQHLQDPGL
                     VPGPEVDA"
     gene            1400..2611
                     /locus_tag="VLR27_01345"
     CDS             1400..2611
                     /locus_tag="VLR27_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSP02114.1"
                     /translation="MPPLHPGDPGDGHGEDGNDPDDVGYTPPLPPEDRLWRHPSEVAA
                     DRAVPAEVPRKGSTDRRTAGLMVLSGLAGATLAVGAVAALGGFETRVVERQVAAQTST
                     VDPLDDGLEDVAHRTAPSVAALLVHRGEEQTPASAVVWRRDGYLVTDGPSVAGADEID
                     VVLQGGDAGVARLVAVDDLTGIAVLHVDAELEHAVLTDDVDPVGVGVRTVAVGASPDG
                     GWDLTVSSGVVSAVGRRLEAPDGATRHGMILVDKAFAPGTAGGALVDRHGVVVGIISG
                     ASASSGGATFGVATPIDLARHVADQLVEHGRAEHVWLGLRGTDLDVDEAMAMGLAGGA
                     LVEAIDDGPAGQAGIEPGDVVVSVDGDPTPTMSALIASLRRHMPGDVVVLGIHRAGET
                     RDVRVTLAGKG"
BASE COUNT          341 a          963 c         1036 g          346 t
ORIGIN      
        1 gccgtgcgct cgccgatggc gatgccgtac tcgcgccgca cgtaggactg gatggcggcg
       61 tcgatgtcga aggagccgac gcgcacggcc tgcagggcga cgatgccgcc gaggctgatc
      121 agcgcggtct ccgtggtgcc accgccgacg tcgatgacca tgttggcgac cggctcgtgg
      181 atcggcagct cggcgccgat ggctgcggcc atgggctgct ccaggagctg ggcgtcggcg
      241 gcgccggccc ggcgggcggc ctcggtgacg gcgcggcgct cgacctcggt gatggccgag
      301 gggacgcaga tcacgacccg ggggcggttg aagcggttca cgcccgcccg ctgcaggatc
      361 aggcggatca tgcgctgggt gatgtcgaag tcggtgatgg cgccctggcg cagcggccgc
      421 acggcgatga tgtagctggg cgtgcggccg atcatctgcc aggcctcccg gcccatggcg
      481 agcacgtcgc cgttgcgctt gttgagcgcg atgacggtgg gctcgttcat gacgatgccc
      541 tggcccttcg cgtacacgag cgtgttcgcg gtgccgaggt cgatggcgag gtcgcgtgcc
      601 acggctactg ggcgtcccgg tgcggggcgt cggggtgctg gtcgtcgggt ccgccgcggt
      661 gctcgggcgg cggcgccagc gctcgcatct cgcgacggaa cgaccggatg ccggattcga
      721 tggagctctc cgggcggccg cggtaggcga gcacgctgac gccgacgacg acgatcacca
      781 cgatgaccaa caggaacggg atcatgcgtc gacctcgggg ccgggcacga ggccgggatc
      841 ctggaggtgc tgggcctcga ggccccagct ctcgccgccg tcccagcgct ccttcttcca
      901 gatcggcacc gactgcttca gcgcgtcgat gcagaaccgg gcggcggcga aggcctcgtc
      961 gcggtggggt gcggacaccg acaccacgac cgcggcctcg gtgacctgga gcgggccgat
     1021 gcggtggagc agggcgatgt tccccaccgt ggcccaccgg gcccgggctt cggcggcgac
     1081 ccgagcgagg cggggcacga cctcggtctc gtaggcctcg tactccagca cctcgacgcc
     1141 gggccggccc tcgctgtggt cccgcgccgt gccgacgaag gtgaccacgc cgccgcagtc
     1201 gggccgcacc acccaatcgg tggccacctg caccggcagg ggttcctcgg cgagcgcgaa
     1261 ccagtcgtgg ccgcgcgccg gaggctcgag ggtggcggag gggatcatct cgtccaagac
     1321 tacgatccgg cgcgcagaac cggtgaagcg catcgggggc gacgtccacg gcccgcaggg
     1381 cgtcggtagg gtcgctccgg tgcctccgct gcatccgggc gatcccggcg acgggcacgg
     1441 cgaggacggc aacgaccccg atgacgtcgg gtacaccccg ccgctgcccc cagaagaccg
     1501 gttgtggcgc cacccgagcg aggtggcggc ggaccgcgcc gtccccgcgg aagtcccgcg
     1561 caaggggtcg acggaccgcc gcacggccgg cttgatggtg ctgtccggcc tggccggcgc
     1621 caccctcgcc gtcggcgccg tcgccgccct cggcggcttc gagacgcgcg tcgtcgaacg
     1681 ccaggtcgcg gcgcagacct ccaccgtcga ccccctcgac gacggcttgg aggacgtggc
     1741 ccaccgcacc gcgccatcgg tcgccgcgct gctggtccac cgcggcgagg agcagacccc
     1801 ggccagcgcg gtcgtctggc ggcgcgacgg gtacctggtg accgacggtc cctcggtcgc
     1861 cggcgccgac gagatcgacg tggtcctgca gggcggcgac gccggggtgg cccgcctcgt
     1921 cgccgtggac gacctgaccg gcatcgccgt gctgcacgtc gacgccgagc tcgagcacgc
     1981 cgtgctcacc gacgacgtcg atccggtcgg cgtcggggtc cgcacggttg cggtcggcgc
     2041 ctcccccgac ggcggctggg acctcacggt gtcctcgggc gtcgtgtcgg cggtggggcg
     2101 gcggctggaa gcaccggacg gtgcgacccg ccacgggatg atcctggtgg acaaggcctt
     2161 cgcgcccggg acagccggcg gcgcgctcgt cgaccgccac ggcgtggtcg tgggcatcat
     2221 cagcggggcg agcgcgtcga gcggtggggc cacgttcggg gtcgccacgc cgatcgacct
     2281 cgcccgccac gtcgccgacc agctcgtgga acacgggcgg gccgagcacg tctggttggg
     2341 cctgcggggc acggacctcg acgtggacga ggcgatggcc atgggcctcg ccggcggtgc
     2401 gctcgtggag gccatcgacg acgggccggc cggccaggcc ggcatcgagc ccggggacgt
     2461 cgtcgtgtcg gtggacggcg atccgacgcc caccatgtcc gccctcatcg cgagccttcg
     2521 gcggcacatg cccggcgacg tggtcgtgct cggcatccac cgggccggcg agacccgcga
     2581 cgtgcgggtc accctggccg gcaagggctg atcagcgcag cgtccgccgc accgccgccg
     2641 cggccccgac gccgccggcc accaccacgg cagcggcagc ggcgag
//
LOCUS       DATISU010000027        27434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_886120, whole genome shotgun sequence.
ACCESSION   DATISU010000027 DATISU010000000
VERSION     DATISU010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 27434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27434
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886120"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VLR27_01350"
     CDS             <1..247
                     /locus_tag="VLR27_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HSP02115.1"
                     /translation="AGHPTVVTVEDGYKAGGAGSLLASGIRECCEERLVPRIEILGVP
                     TQYIDHAKVDVIMGRLGLDADGIRSSVLRTVRAPSPL"
     gene            complement(688..1353)
                     /locus_tag="VLR27_01355"
     CDS             complement(688..1353)
                     /locus_tag="VLR27_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HSP02116.1"
                     /translation="MATTESEAPQTTTVDDAPVTDDEVVLTVTETAIAKILELRESED
                     DPADLALRIAVTGIRGPEYTYDLTFEAVAEASETDIVYHQGELAVMVPADSMDSLQGA
                     TLDLPGTDGQGGLVLRNPNRPDPLAGIDLDLTGSTAEKVQQLLEQQINPGLAAHGGFA
                     ELKGVDDDKVYVLMGGGCQGCAVSAMTLREGIAASITAAIPEITEVIDVTDHDAGENP
                     YYS"
     gene            1414..2232
                     /locus_tag="VLR27_01360"
     CDS             1414..2232
                     /locus_tag="VLR27_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HSP02117.1"
                     /translation="MSDETTTDEPDPVLYEVADHVATITLNRPHRRNAISTRMLSMLS
                     DALTEADRSADVRAIVLTGAGKGFCAGLDIKDAMSGSGIGSEGSGAAANMMNTRDLPT
                     SILFEIDTPIIAAVNGAAAGYGLDLALGCDMRILGEGSRLLPGFAKRGVVPESGGTWY
                     LPRLVGWAKAAEIGFLGRDLDAQQSLELGLANAVVADDALVAEAVSWGKEIATNAPLA
                     IRAMKRLYRHGLTEDFPAHTHHVLLQTMQLFGTKDFFEGISSFAEGRDPEFHGR"
     gene            2466..3470
                     /locus_tag="VLR27_01365"
     CDS             2466..3470
                     /locus_tag="VLR27_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02118.1"
                     /translation="MLPGRVLDLFARQYSLIGRHQLLDRCTAAERRRLVRHPDIDRAG
                     PRVLRHRAGVVELGQQLLLPVLDAGPGALLWGHAATHWWGFGRFRAFPVHVTRPRNGT
                     RTPHIGIVHVTKHLDPVLDATVHRGIPIVRPERLLLWLARADTERYGHDIGALRLERA
                     LDHAWRERLIDGHRIHELAARCSGKGNSGIVILRQLLESRPPDYRPTDSNLETRWEEV
                     VGPLARSFRRQIVLGDDAPIGRFDQVHVDRPLVVEIHSEKHHTMPSDVADDEDRADRL
                     VEAGFAVVIYWEHDIWHDAPTVRRTLRAILARPHEARIHRPTRAPHLALPVPGAPNDR
                     "
     gene            complement(3570..4352)
                     /locus_tag="VLR27_01370"
     CDS             complement(3570..4352)
                     /locus_tag="VLR27_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSP02119.1"
                     /translation="MSNGELQGRVALVTGGGRGVGRAISLALAEAGADVAVNYRRDEE
                     SAVNTAAEIQDMGRKAIAVQASIDDADADAEMVERVTGELGPVSILVNNAGLASRGQT
                     VADTDPSEAARLLATHAVGPHHLCRLVLPGMRTCDRGDIVFISSVATDHHAANGAPYN
                     MGKAAMEALASTLAKEEQRHGIRVNTVAPGLVETEMGRRLAKAGGADIEDLHAHYPFG
                     RVCQPEDVAAVVSFLVGEQGSYVSGVRIRVDGGGQQMIRPPR"
     gene            complement(4376..6166)
                     /locus_tag="VLR27_01375"
     CDS             complement(4376..6166)
                     /locus_tag="VLR27_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HSP02120.1"
                     /translation="MLVDATGLRAARPDRVLFEDLSLAIASGDRLAVVGVNGTGKSTL
                     LGALAGSRTPDDGTIRRGRGARIAHLDQDAPLPAGTVASVTGDRWEAAAVRDRLGLAG
                     LEDRPTDELSGGQIKRVALARALLECGPPGGGPPDDADLLILDEPTNHLDVDAIAWLE
                     EWLSTFRGGLLMVTHDRHVLDRVCNRMLELDRGGSYLHEGGYASYLEAKAERIDRAAS
                     AETKRKNLAKVELAWLRRGAPARTSKPKHRIESATALVEGGPSGGDVRDQALALHQGT
                     PRLGDKVIEIHGLGADVEGRTLFDGVDLLLDPRERLGIVGPNGAGKTTMLRILAGERE
                     PERGTIDVGPTVRLAMFSQDAAELDPDARVRDLVTGGKKEVSWQDTALMERFWFDSDT
                     QWAPVSTLSGGERRRLQLLLVLLQQPNVLLLDEPTNDLDLDTLRLLEDFLDEWPGALI
                     VVSHDRALLERTVADVLVIDGKGGAARRPGGLAAYLESRRTNRAKGKTEAKAPSGSKA
                     AQPAAAPAQRSTSPSTLRRELNAAERNLAKATTEVERIEAELHDAIGDHQRLSDLGQQ
                     LATARAAMDAAEERWLTLAEEAEAVNPGLL"
     gene            6203..7393
                     /gene="dusB"
                     /locus_tag="VLR27_01380"
     CDS             6203..7393
                     /gene="dusB"
                     /locus_tag="VLR27_01380"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HSP02121.1"
                     /translation="MDSDRRWWWIDEIFLGDWSTVPLFPPLQIGPLSADPPVVLAPMA
                     GVTNAPFRALCRRFGAALYVSEMVGARAVVEGNERTFHIARFADDEPVRSIQLYGTDP
                     AVMGEAVRRVRDQVGVDHIDLNFGCPARKITRHGGGSALPLRPRLFGAVVAAAVDGAG
                     DIPVTVKMRVGLDDEHRTWLVAGKMAEEAGAAAVAVHARTAEQLYSGVADWSTITQLK
                     ETVTTIPVLGNGDIWEAADAVEMMRVTGCDGVVIGRGCLGRPWLFRDLVAAFAGEPVP
                     DPPSLGEIIEMVRTHGEMLAEWFGEESGLRELRKHTGWYLQGFPVGRPLRAALRSMST
                     LAELDALLGQLDPHLPFPRSAMRMQRGHTTGPRPVKLPEGWLDDPDALEPPSRLADLA
                     VSGG"
     gene            7396..7971
                     /locus_tag="VLR27_01385"
     CDS             7396..7971
                     /locus_tag="VLR27_01385"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HSP02122.1"
                     /translation="MGATVVLASGSPRRLELLRRIGVEPVVRPADIDETPGPDESPAA
                     TVARLARTKAHTVEHTPDDLVVAADTEVVLRDAVLGKPADADAAAAMLRSLSGRAHRV
                     VTGVHVVCGEREAAAVEETIVHVRELTDAEIDAYVATGEPFGKAGAYAIQGAGGMFVE
                     RIEGSDSNVIGLPLATVVRLAGDVGVTILPR"
     gene            complement(8010..10382)
                     /locus_tag="VLR27_01390"
     CDS             complement(8010..10382)
                     /locus_tag="VLR27_01390"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HSP02123.1"
                     /translation="MQLARWCYAHRRPVVVVWIAAVVLTGVAVAIVGSDFTGNPEAPE
                     SESRDGFEVLEEHFDGLGAGPAGSIVFQADQGVTDPEVQAAMEELFAETDEIPFVEVT
                     SPYAPEAFGQIAEQGELAGRVAFARVEVGEEVGFSQSGEIGVEISDRAEEIESEIDGV
                     RIEVGGEVLAGFEPPEAELIGLAFAVLVLIVSFGSVLAMGLPIAVALAGVGLGIMLTN
                     LISNVTPMPDFALQLGAMIGLGVGIDYALFIVTRFREYLHRDHDPEVATMAAIDTAGR
                     AVVFAGITVVVSLLGLLLIGLEFVAGMGLAAAITVAVTMVASTTLLPALLGFAQLKVE
                     MTRWRGLVAAACVSVALLGAGLGIPMLLLGVPLAIVVLVVGTFLPLLRREVPRRTPKP
                     VRETGWYRWSHLIQRRPWPFAIGGTLLLLVLALPLLGIRLGFSDEGNYPEETTTRQAY
                     DLLSDGFGPGFNGPFVVVAEVDGPEGFATFQQVVEAVSSDPGVASVTPALPNDFEQPV
                     AALARVVPTSAPQDEATEDLINRLRGEVIPSVVGDGGPDVHVTGFVAVSVDFSDYLAG
                     RSFLFFGVVLAMSFVLLMAVFRSLLVPLKAVILNMLSIAGAYGIVVAIFQWGWLGDLT
                     GVEPAPIEPFIPMMMFAIVFGLSMDYEVFLLSRIKEEYERTGDARNSVADGLAATARV
                     ISAAAAIMVVVFGSFLLEDSRIIKVFGLGLGMAVLLDATLVRMLLVPATMELLGERNW
                     WLPRWIDRLLPALHVEGAPDHEQQLATAAARVDADRGDESDDVREPVGVS"
     gene            complement(10420..11004)
                     /locus_tag="VLR27_01395"
     CDS             complement(10420..11004)
                     /locus_tag="VLR27_01395"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HSP02124.1"
                     /translation="MTSTTVRTTSIETPTGPVRLAVAVDDDGERVVALAFADHFVRVA
                     AKVRVRFAHAEWVEGETAAADAVRRYLDGDLAAVDDIAVDATGTPFQQQVWDALRAIP
                     AGETRSYAQIAAAIGSPAAVRAVGTANGANPIWLVVPCHRVVRSDGSVGGYGGGPDRK
                     EWLLDHERSTKGANGTDVTSRSSAASVKVSPDRP"
     gene            complement(11001..12440)
                     /locus_tag="VLR27_01400"
     CDS             complement(11001..12440)
                     /locus_tag="VLR27_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HSP02125.1"
                     /translation="MSLDHQQCYEALRSRDARFDGRFWVGVRTTGVYCRPVCPSRTPL
                     SRNVDFFAHPAAAEDAGLRPCRRCRPEAAPGSSGWKGAAGVVDRAVRLIDEGALDDGD
                     VAALARRLHVGDRHLRRLFVEHLGTTPDAVARSRRAHLARRLLADTDLRVADIAFAAG
                     FGSVRQCNDVLKATFRATPTELRADLRPTGRTPSRTGSAGVASCALTLRLAVRPPFDG
                     AALLDWFAARAVAGLEVVDAETYARRVSVGDRTGSVTIRPGADAVVLGVDLPPDTHLG
                     SLVAGARRAFDLGADPVAVDEHLGADPLLGPLVARRPGIRVPGSWGGFEAGVRAVIGQ
                     QISVAAARTVLGRIVAAHGRDGAFPEPEDLAGAPLEQLGIIGQRADTIRRLAESVLTG
                     AVVLDGSVPHEELVASLVALKGIGPWSAEYVALRLGEPDAFPAGDLHLRRMLDATNDR
                     TAIARAEPWRPWRAYAAMHLWQTPREERP"
     gene            12537..13241
                     /locus_tag="VLR27_01405"
     CDS             12537..13241
                     /locus_tag="VLR27_01405"
                     /inference="COORDINATES: protein motif:HMM:NF004858.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HSP02126.1"
                     /translation="MTDPVQLTIEDGVAVVRLDDGKANAVSHTLIDQVHAALDTAQAD
                     ATAVAFIGREGKFSAGFDLSVMTQGADATRGLVKAGGELLMRVYTHPQPTVAAVTGHA
                     LAAGALLCLASDTRIGSADLPAKIGLNETAIGMGLPWYGIRLGEARLSLRHRQRSVLQ
                     AEIYDMAGALDAGYLDELVDADAVVDTAIEQARRLGQLPGHAYAFTKRRLRQALADEV
                     LLGLDADMETMVPPGS"
     gene            complement(13317..14378)
                     /locus_tag="VLR27_01410"
     CDS             complement(13317..14378)
                     /locus_tag="VLR27_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSP02127.1"
                     /translation="MTGQDLPPFDLAAHLRDALGVDPELVAAAEVDGPEQLRRLAQAV
                     VLLGEPPRLRPPDAWQRTGADEELARALWRAMGFAHVPDEAVALTDADVTALAAINDF
                     LQATGISSETAIRFARLLGQTMSRVADALHSIVDHAIDEMDALPPGENDDLMVLAAQL
                     VNPLIEQELSYLLRRHLYAGAIRRIAGHDLEQPDVTVGFADVVSFTRLSGQLAEADLA
                     ELLETFEATTADMITERGGRVVKLIGDAVLFVFDDPEAAAHLALELVESFGGDQPDLR
                     VGLTHGSVLSRLGDLFGPPVNLASRLVSYARPGSVLVDQMFVDRIGEVDDLRFRSIRP
                     RDLKGIGTTALYVLRRDER"
     gene            complement(14386..15015)
                     /locus_tag="VLR27_01415"
     CDS             complement(14386..15015)
                     /locus_tag="VLR27_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02128.1"
                     /translation="MHRRLRHRRLALALVLVGSLLAAGCGDDDAGDDGAAPEDGTEER
                     DERSERGPRFCDAYLEYLAEPSPEHLDTVVESADDAEVDELAAIIAEDDRTGRVLAAD
                     SDLQAIARDQCQAEWVGAAQGGGDTAGAAQAFFDALVAGDPIGARNVASANAIAVFEP
                     WAPVEPDPDAGTPALLEIGDRAFSFALDPATIAECQAEAGVVIACTVAE"
     gene            complement(15044..20512)
                     /locus_tag="VLR27_01420"
     CDS             complement(15044..20512)
                     /locus_tag="VLR27_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HSP02129.1"
                     /translation="MVDFRRIAIVNRGEAAMRLVHAVRELDGSFTTIALHTRAERRAM
                     FVREADEAVCFEDLGVPIAGTPYLDLDVLAAALTAARAEAAWVGWGFVAERPEFAERC
                     AELGVAFIGPSAECMRLLGDKIEAKRLAEEAGVPLAPWSGGPVADVDEAVAAGERVGY
                     PLMVKAAAGGGGRGIRRVDDPAELASAFERARDEGAKSFGDPTVFLERVVSDARHVEV
                     QIIADQHGNVWAPGVRDCSIQRRNQKVIEESASTALGPGHEARLRDAAVRLAKASGYV
                     NAGTVEFLYQPEEDLLAFLEVNTRLQVEHPVTELTTGLDLVRLQIEVALGHPLVGEPP
                     EPNGHAFEARLNAEDPQRGFAPAAGRIERLVLPTGPGVRVDTGVAEGDVIAAEYDSMI
                     AKVIAWGADREQARRRLRRALSQTTVLVEGGTTNKSFLLDLVDRPEVVVGDVDTAWLD
                     RLTGSDGHRTDRFAEIALLVAAIDVHDHERLLDRARFLSSAARGRPESDLEAGHDVEL
                     RWEQDEYRLHVATGDLTGWYRVTMDGVVADVVLDRLDDAHSRLVVGGRTYRVVSHAHR
                     TEHLVEVEGVSHRFSRDDGGLLRAPAVALVVSVEVRPGDQVVAGQRVAVVEAMKMETA
                     VVAPSDGVVEEVFVSPNVQVDVGAPLLRIGASDGNGEHDEGTRQRLRIAPAGRASETD
                     RTTSRLDVLRSLLLGFDVADRDDRIIEAHRDEADAADPTVRRRELELLRVFADLCGLT
                     RDRRGSEGDHGLELRSPLEHFHAYLRTLDADHESLPERFRTRLQDALAHYGVRSLDRT
                     TALEAAVHSIHRAVQRRQEQLPVVQALLERRLDHCGDPEERDAVRDTLDRLIAATQAQ
                     YPAIGNLARSVRHRCIDRPLLDHARADVHDEVRRSLRALADDPGDRAASDRLVATPVP
                     LMSVIAGEDALGRDAALSAAIVEVLTRRFYKIRDLEGLERHLGALPAVTAGYEHRGRR
                     VEVIGVACDEATLGDAVADVTRRAGGDATHVVDLYVRLAEPRTADDLVARVDEAVAAA
                     DIPGAVERIAIVAAAAGADHGEVHHLSWDRTAEGSFREVTVFRGLHPMIGQRLQLWRL
                     ENFEVARVPGPEDVHVFDCVSVEQPGDERLVAVAEVRDITPVRDASGALIALPELEQV
                     LVSCLDGIRRSLSTDRRRRRLEWNRVMLFVWPTVEISLEEVTEVAKRLVPLTNGLGIE
                     QVLVQGRVTDPGSGDTADVVFRLGFQAGRGLTMQVTEPPTAPMQPLDDYTQKVLACRR
                     RGTVYPYEIVPMLTTEAEHGEGHFTELDLDGDRLVPVDRPPGHNVAGVVVGLVTAATE
                     VHPEGMTRVAILGDPTRSMGSIAEAECRRLLAAIDLAEQRGLPVEWFALSAGARIAMD
                     SGSENLDWVASVLRRIVEFTQAGGEINVVVAGVNVGAQPYWNAESTMLMHTKGILVMT
                     PDSTMVLTGKQAIDFSGGVSAEDNLGIGGHERVMGPNGQAQYWAPDMVGAVDILFAHY
                     DHSYVAPGEPGPRPTLTTDPIDRDVTDEPHAGAGTTFTTVGEIFSDATNPGRKKPFDI
                     RSVMRAVIDHDLEPLERWADMAEAEGAVVLDAHLGGLPICMVGIESRPLPRTGQLPAD
                     GPSQWSAGTLFPLSSKKVARAINAASGNRPVVVLANLSGFDGSPESLRRLQLEYGAEI
                     GRAIVNFDGPIVLCVISRYHGGAFVVFSGRLNDRMQVLAVEGSHASVIGGAPAAAVVF
                     TREVDARTQADARVAELEQRLATAPPQERARLRAELDAVRSSVRNEMVGEVAAEFDRV
                     HSVERARDVGSVHEIIEPARLRPALHEAVSRGLTSVDG"
     gene            complement(20555..21079)
                     /locus_tag="VLR27_01425"
     CDS             complement(20555..21079)
                     /locus_tag="VLR27_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02130.1"
                     /translation="MAGAVVLATVLAGCGDDDAEPAAAEEVAVCAELRQRENELIQVA
                     NEALSELARAEDDETRAAALADGYDRLVETLAAQDPVVVDGEPELTERFAAGQRSAVA
                     ELERERDRFVAEVRAVTQADEHGRAGELQNALEKAFSELEPPRSAYVAAGLDEAIDAD
                     PGCRHVTQRGDPAR"
     gene            complement(21120..21806)
                     /locus_tag="VLR27_01430"
     CDS             complement(21120..21806)
                     /locus_tag="VLR27_01430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HSP02131.1"
                     /translation="MFQPGELSPRVRNLAAGAIQRSWEWICAVGATSPDDAHGRRFRS
                     MGERSMIAFPPGQLFNEHWIEIGAFTLIGPHVSMSVGMWNEDLDPSAPPVIRIGDRCN
                     IGRGGSLVARIGIAIGDDVTIAPDLYVTDHNHAYDDVEVPIARQWPHEAPVSIGSGSW
                     IGAGVVILPGTTIGEHVTVAAGSVVRGDIPDRSVVAGVPGRVVRRWHDGAWDPPLEPR
                     ATEPPDDWPS"
     gene            21838..24459
                     /locus_tag="VLR27_01435"
     CDS             21838..24459
                     /locus_tag="VLR27_01435"
                     /inference="COORDINATES: protein motif:HMM:NF023454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HSP02132.1"
                     /translation="MARRLVDFLPRPTGGEPDPDAIIEAFTDWAIERGLELYPHQEEA
                     ILELLSGSNVILATPTGSGKSLVALAAHAKSVAGGGRSWYTAPVKALVSEKFFDLCEQ
                     LGPENVGLLTGDSSVNADAPVICCTTEILAHVALKDGVEADAETVVLDEFHFYGDPDR
                     GWAWQVPLLELPQSSFLLLSATLGDVRFFREDLTRRTGRPTALVDDAVRPVPLHFEYR
                     RTLLHHTIEELLAKDAAPLYLVNFSQKDAVAQATAMASVTKLDDDAKEIVTAELTKAK
                     LPAGFGRDLARLLRQGVGVHHAGMLPRYRRLVERLTQMGVLKVISGTDTLGVGVNLPI
                     RTVVLTRLYKYDGTDTRVLTAREFHQIAGRAGRAGYDTAGLVIALAPEHVAENEVAQA
                     KAADDPKKKKKLKKSQPPKGFVHDDEETFTTLQEAKPEPLESSFRMTPGMLRQLLDRP
                     GDAWAHGRSLLLDSHEPRSRQRRHVRSTIGLYKALRTAGVVRLLDEPDKYGRFVEVDH
                     ELQDDFALNQLLAPFLLHAVPLRDRDDEGYPYAVLALVEAVVDNPFPILMAQKDKLKD
                     EAMAEMKAAGVEYEQRIEELDKIQYPQPMREQIYDVFDIWRVANPWIGDRNIAPKGVV
                     RDLWDRAMDFPAFVRHYGIKRSEGLLLRYLSDVYKTMLRTVPDEAKTPEVVELQDWLG
                     AVIRATDSSLLDEWTAMLAGGDADPASLAREVAEDPGIPDVTTDRHAFRALVRTRIFR
                     WVQYAATGQWTAWSEDLTEVGDRGWNERKLLEQFAPYLEAYGDDAPQPDPDAPTARPG
                     EERPAVVPRPRIGIDGDARGPARFVVADEGDEWEIEQVLVDPEGHDEWYLAVTIDLPA
                     SAEQGDVVAHLEGLRRR"
     gene            complement(24496..25407)
                     /locus_tag="VLR27_01440"
     CDS             complement(24496..25407)
                     /locus_tag="VLR27_01440"
                     /inference="COORDINATES: protein motif:HMM:NF037405.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HSP02133.1"
                     /translation="MSAELVAAGRILAHQQSLITTSQAEGVGIGRKRCQRLVDRGLWE
                     RLDLGVYGPTGVALTWQRRLMLALLLAPDGSLGSHRSSAALLGVGGFTRPSPEVTIPR
                     GTTLRRPWLVVHESTDLELADRRRIDGIPITGPRRLAMDLGSVVSPSQYRQAMRGLRA
                     SHGVQFPALLRTYLRHKRSGRNGGAALRDWLDRYVDVGGVPESGLEQVVLDAFLDAGL
                     RPVAQHWVEVGPNRYRLDLAFPDLMVAIEIDGSQHEEKPAQDADAIRQGALEAVGWTV
                     ERIRVATFTADLVRAIEAVRRASVVAR"
     gene            25542..25997
                     /locus_tag="VLR27_01445"
     CDS             25542..25997
                     /locus_tag="VLR27_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HSP02134.1"
                     /translation="MARLLIVHHTPSPSLHTMLGAIRDGAGADGLEEVEVVTAAALSC
                     TAADVLAADGYVLLTPANLGTMAGALKHFFDTIYYPCLEETQGRPFGCAVHGNSDVDG
                     ALRDVTKITTGLGWSQATDHVRVLDTPEAEARDACWNLGATVAATVLET"
     gene            complement(26058..26846)
                     /locus_tag="VLR27_01450"
     CDS             complement(26058..26846)
                     /locus_tag="VLR27_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02135.1"
                     /translation="MQKRAIAAFVALALVITACGDDDDEVTEGDTSAETDDTTAETED
                     TAETDDTTASGEGEQAAGDGEFCSAYQELLAGDPTPDAIREVAGIAPDGAVEPLETLA
                     AGFEDDPEGFFDTEEFGAAFSEVGSVANKECADEVIAVTAIDYGYEGIDAELSVGVYG
                     VDFSNEGDEFHEMIVLRKGDDTTESFEDILALDEEAAMALVQEIGATFAPPGGESGGL
                     FDMSEPGEYAAICFIPVGSTPDAEAESEGPPHFTQGMIAEFTVS"
     gene            complement(26950..27216)
                     /locus_tag="VLR27_01455"
     CDS             complement(26950..27216)
                     /locus_tag="VLR27_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02136.1"
                     /translation="MRSALRRTSSFALALALVLGGAAGLSACGDDDGADVREIGETEG
                     SGSGSGSGSGSGSGSGSASGVESETEDTGSASEADDTTSTTDAP"
BASE COUNT         3797 a         9892 c        10042 g         3703 t
ORIGIN      
        1 cgccgggcac cccaccgtcg tcaccgtcga ggacggctac aaggccggcg gtgccgggtc
       61 gctgctcgcc tccgggatcc gggagtgctg cgaggagcgg ctcgtccccc gcatcgagat
      121 cctgggcgtg cccacccagt acatcgacca cgccaaggtc gacgtgatca tgggccgcct
      181 cggcctcgac gccgacggca tccggtcctc ggtcctgcgg accgtgcggg cgcccagccc
      241 cctctaggac cccgcacctg gggcgccttc gctggggtcg gagaacgaag gcgccccggg
      301 cagggaacac aacgccggga gggaaccgtg gcgttgctgg gtggggaacg gccgtcgtcg
      361 acggaccttg cggtgggatc cggaatttct ccgcgccccc gtcggagccg cacgcgacga
      421 ggcccggacc gtggtccggg cctctccccc tggtcgcccc cacttggaag gtgacaacga
      481 acacagcatt ccagccgagc gcgcagaatg caagcggcga cggtcgtcga ccgtcgccgc
      541 ttgcacgttc cgcccagtta cgggcagtta cacacgcgcg aaggtcaggc ccggagccga
      601 agccgaaggc gaggcgaccc agaggcacgg ttggttcggc ggcgaagccg ccatctcgcc
      661 gtcggtgggt ggaacgaccg cgaccagcta cgagtagtag gggttctcgc cggcgtcgtg
      721 gtcggtgacg tcgatgacct cggtgatctc ggggatcgcc gccgtgatcg acgccgcgat
      781 gccctcgcgc agggtcatgg ccgacacggc gcagccctgg cacccgccgc ccatgaggac
      841 gtacaccttg tcgtcgtcga cgcccttgag ctcggcgaag ccgccgtggg cggcgaggcc
      901 ggggttgatc tgctgctcga ggagctgctg gaccttctcg gcggtggagc cggtgaggtc
      961 gaggtcgatg ccggcgaggg ggtcgggccg gttggggttg cgcagcacga ggccgccctg
     1021 gccgtcggtg ccgggcaggt cgagggtggc gccctggagc gagtccatcg agtcggccgg
     1081 caccatcacc gccagctcgc cctggtggta cacgatgtcg gtctcggagg cctcggcgac
     1141 cgcctcgaac gtgaggtcgt aggtgtactc cgggccgcgg atgccggtga ccgcgatgcg
     1201 cagggccagg tcggccgggt cgtcctcgga ctcccgcagc tccaggatct tggcgatcgc
     1261 cgtctcggtg acggtgagga ccacctcgtc gtcggtgacg ggcgcatcgt ccaccgtggt
     1321 ggtctggggt gcctcggact cggtcgtggc catgtgccta cgctaccgac gaaccatcgg
     1381 aagaccatga gccgaccggc tcgggggcgt gacatgagcg acgagaccac caccgacgaa
     1441 ccggatccgg tcctctacga ggtggccgac cacgtcgcca cgatcacgct caaccggccc
     1501 caccgccgca acgcgatctc cacccgcatg ctcagcatgc tcagcgacgc cctcaccgag
     1561 gccgaccgca gcgccgacgt ccgggccatc gtgctcaccg gcgccggcaa gggcttctgc
     1621 gccggcctgg acatcaagga cgccatgtcg ggctcgggca tcggcagcga gggctccggc
     1681 gccgccgcga acatgatgaa cacgcgggac ctgccgacct cgatcctgtt cgagatcgac
     1741 acgccgatca tcgccgcggt caacggcgcc gccgccggct acggcctcga cctggcgctc
     1801 ggctgcgaca tgcgcatcct cggcgagggc tcccggctcc tgcccggctt cgccaagcgc
     1861 ggcgtcgtgc cggagtcggg cggcacctgg tacctgcccc gcctcgtggg ttgggccaag
     1921 gccgccgaga tcggcttcct cggccgggac ctcgacgccc agcagtccct cgagctcggg
     1981 ctggccaacg ccgtcgtcgc cgacgacgca ctcgtggccg aggccgtgtc ctggggcaag
     2041 gagatcgcca ccaacgcccc cctcgcgatc cgggccatga agcgcctcta ccgccacggc
     2101 ctcaccgagg acttccccgc ccacacccac cacgtgctcc tgcagaccat gcagctgttc
     2161 ggcacgaagg acttcttcga ggggatctcc agcttcgccg agggccgcga ccccgagttc
     2221 cacggccgct agaagctcgc ccccagtcgt tcctcacgcg cggaacgact gcaggcagtc
     2281 gaatcgcgcg cgggaaacgg tcccccaccg gttcccggcg cgcagaacga ctgtccttgg
     2341 tcgttctgcg cgcacggaac ccgatggggt gcggaccccc gttggctgtc ggtgggtgcc
     2401 ggtaccgtcg acggtgtcgg tccgccggct cgcttccccc agcgcaccga ccatcggagg
     2461 tcaccgtgct accgggacgc gtcctcgacc tgttcgcccg ccagtactcg ctcatcgggc
     2521 gtcaccagct gctcgaccgc tgcacggccg ctgagcggcg tcgcctcgtc cgccacccgg
     2581 acatcgaccg tgcggggcct cgtgtcctac gccaccgagc cggcgtcgtg gagctcggac
     2641 agcagctgct cctccccgtg ctcgacgccg ggcccggcgc gctgctctgg ggtcacgccg
     2701 ccacgcactg gtggggcttc ggccggttcc gcgcctttcc ggtgcacgtg acccgacccc
     2761 ggaacgggac gcgcacgccg cacatcggga tcgtgcacgt caccaagcac ctcgatccgg
     2821 tcctcgacgc gacggtccac cgaggcattc ccatcgtgcg gccggagcgc ctgttgctct
     2881 ggctggctcg cgccgacacc gagcggtacg gccacgacat cggcgccctg cggctggagc
     2941 gggccctcga ccatgcctgg cgcgagcgcc tgatcgacgg gcaccgcatc cacgagctgg
     3001 ccgcccgctg ctccggcaag ggcaacagcg gcatcgtcat cctgcggcag ctgctcgaga
     3061 gtcgcccgcc cgactaccgg cccaccgaca gcaacctcga gacgcgttgg gaggaggtcg
     3121 tcggaccgct ggcccggtcg ttccgcaggc agatcgtgct cggggacgac gcgccgatcg
     3181 gtcgcttcga ccaggtccac gtcgatcgtc ccctcgtcgt cgagatccac agcgagaagc
     3241 accacacgat gccgtcggac gtcgcggacg acgaggaccg ggcagaccgc ctcgtggagg
     3301 cggggttcgc agtcgtcatc tactgggagc acgacatctg gcacgacgcg ccgaccgtcc
     3361 gccgcaccct ccgggcgatc ctcgcgcggc cgcacgaggc ccgaatccat cgaccgaccc
     3421 gtgccccgca cctcgccctc cccgttcctg gcgcgccgaa cgaccgttga cggtcgtgtc
     3481 gcgcgccagg aatggtgggg atcgggttca gtgcgcgccg atcgaccggc gacggtcgga
     3541 tcgcgcgcga ggaacgagat cggggtgggt cagcggggtg ggcggatcat ctgctggccg
     3601 cccccgtcga cgcggatgcg cacgccgctg acgtaggagc cctgctcgcc gacgaggaac
     3661 gacacgacgg cggcgacgtc ctcgggctgg cagacccgac cgaacgggta gtgcgcgtgc
     3721 aggtcctcga tgtccgcgcc gccggccttg gccaggcgcc ggcccatctc ggtctcgacc
     3781 aggccggggg cgacggtgtt cacccggatg ccgtggcgct gctcctcctt ggccagcgtc
     3841 gacgccaggg cctccatcgc ggctttgccc atgttgtacg gggcgccgtt ggcggcgtgg
     3901 tggtcggtgg ccaccgagga gatgaacacg atgtcgccgc gatcgcacgt gcgcatgccc
     3961 gggaggacca gacggcacag gtggtggggg ccgacggcgt gggtggccag caggcgggcg
     4021 gcctcggagg gatcggtgtc ggcgacggtc tggccccggg aggccaggcc ggcgttgttc
     4081 acgaggatgg agaccggacc gagctcgccg gtgacgcgct cgaccatctc ggcgtcggcg
     4141 tcggcatcgt cgatcgaggc ctggacggcg atggccttgc ggcccatgtc ctggatctcg
     4201 gcggcggtgt tcaccgccga ctcctcgtcg cgccggtagt tgacggcgac gtcggctccc
     4261 gcctcggcga gcgccaagga gatggcgcgg cccacccccc gtccaccacc ggtgaccagc
     4321 gcgactcgac cctgcagctc tccgttgctc atggcggcgg agcgtagggg ctcgctcaga
     4381 gcaagccggg gttgaccgcc tcggcctcct cggccagcgt caaccaccgc tcctcggcgg
     4441 cgtccatcgc cgcccgggcc gtggccagct gctggcccag atcgctgagg cgctggtggt
     4501 cgccgatggc gtcgtgcagc tcggcctcga tccgctcgac ctccgtggtc gccttggcca
     4561 ggttccgctc agcggcgttc agctcgcggc gcagcgtgga cggcgacgtg ctccgctggg
     4621 ccggggccgc cgccggctgt gcggccttgg agccgctcgg ggccttggcc tcggtcttgc
     4681 ccttggcccg gttcgtccgg cgggactcga ggtaggcggc gaggccgccg ggccggcgag
     4741 cggcgccgcc cttcccgtcg atcacgagca cgtcggccac ggtgcgctcg agcagggccc
     4801 ggtcgtggga gacgacgatg agcgcgccgg gccactcgtc gaggaagtcc tcgagcaggc
     4861 gcagcgtgtc gaggtcgagg tcgttggtgg gctcgtcgag cagcagcacg ttgggctgct
     4921 ggagcaggac gagcagcagc tgcaggcggc gccgctcgcc gccggacagg gtgctgacgg
     4981 gcgcccactg cgtgtcgctg tcgaaccaga accgctccat gagcgcggtg tcctgccagg
     5041 acacctcctt cttgccgccg gtgacgaggt cgcggacccg ggcgtcgggg tcgagctcgg
     5101 cggcgtcctg gctgaacatg gcgaggcgga cggtggggcc gacgtcgatc gtcccccgct
     5161 ccggctcgcg ctcgccggcg aggatccgca gcatcgtggt cttgcctgcg ccgttcggcc
     5221 cgacgatgcc gagccgctcg cgggggtcga ggaggaggtc gacgccgtcg aagagcgtgc
     5281 ggccctcgac gtcggcgccg aggccgtgga tctcgatgac cttgtccccg agccggggcg
     5341 tgccctggtg gagggcgagg gcctgatccc ggacgtcacc gccggatggg ccgccctcga
     5401 ccagcgcggt ggcggactcg atgcggtgct tcggcttgga cgtccgggcg ggcgcgcccc
     5461 ggcgcagcca ggccagctcg accttggcca ggttcttgcg cttggtctcg gcactggccg
     5521 cccgatcgat gcgctcggcc ttggcctcga ggtacgacgc gtagccgccc tcgtgcaggt
     5581 aggagccccc gcggtcgagc tcgagcatgc ggttgcacac ccggtcgagc acgtggcggt
     5641 cgtgggtcac catcagcagc ccaccccgga aggtgctgag ccactcctcg agccaggcga
     5701 tggcgtccac gtcgaggtgg ttggtgggct cgtcgaggat gagcaggtcg gcgtcgtcgg
     5761 gcggcccgcc accgggtggt ccgcactcga gcagggcccg ggccagggca acgcgcttga
     5821 tctggccgcc ggacagctcg tcggtcggtc ggtcctccag gccggcgagg ccgaggcggt
     5881 ctcggacggc ggcggcctcc caccggtcgc cggtgacgct cgcgacggtg ccggccggca
     5941 gcggggcgtc ctggtcgagg tgggcgatgc gggcgccccg gccccgtcgg atggtgccgt
     6001 cgtcgggcgt gcggctgccg gcgagggccc cgaggagggt ggacttgccg gtgccgttca
     6061 cgcccaccac cgccaggcgg tcgccggagg cgatggccag cgagaggtcc tcgaagagca
     6121 cccggtcggg tcgggcggca cgcagaccgg tggcatcgac gagcatcacg accgtcgatc
     6181 gtgccacccg acccgacatc ccgtggattc cgaccgccgc tggtggtgga tcgacgagat
     6241 tttcctggga gactggtcga ccgtgcccct gttcccgccc ctgcagatcg gcccgctgtc
     6301 ggccgacccg ccggtggtgc tcgccccgat ggccggcgtc accaacgccc ccttccgcgc
     6361 cctgtgccgc cgcttcggtg ccgccctgta cgtgtcggag atggtcgggg cccgggccgt
     6421 ggtcgagggc aacgagcgca cgttccacat cgcccgcttc gctgacgacg agccggtccg
     6481 atccatccag ctctacggca ccgacccggc cgtcatgggc gaagcggtgc gccgggtgcg
     6541 cgaccaggtg ggcgtcgacc acatcgacct gaacttcggc tgcccggccc gcaagatcac
     6601 ccgccacggc ggcggttcgg ccctccccct gcggccccgc ctcttcggcg ccgtcgtcgc
     6661 cgcagccgtg gacggcgccg gcgacatccc cgtcacggtg aagatgcgag tcggcctcga
     6721 cgacgagcac cgcacgtggc tggtggccgg gaagatggcc gaggaggccg gcgcggctgc
     6781 ggtggcggtc cacgcccgca cggccgagca gctctactca ggcgtggccg actggtcgac
     6841 catcacccag ctgaaggaga cggtcaccac catcccggtg ctcggcaacg gcgacatctg
     6901 ggaggccgcc gacgccgtgg agatgatgcg cgtcaccggc tgcgacggcg tggtcatcgg
     6961 ccggggctgc ctgggacgtc cctggctgtt ccgggacctg gttgcggcct tcgccggcga
     7021 accggtgccc gatcccccgt cgctcggcga gatcatcgag atggtgcgca cgcacggcga
     7081 gatgctggcc gagtggttcg gcgaggagtc cggcctgcgg gagctgcgca agcacaccgg
     7141 gtggtacctg caggggttcc ccgtcggccg gccgctgcgc gccgcgctcc gctcgatgtc
     7201 cacgctggcc gagctcgacg cgctgctcgg ccagctcgac ccgcacctgc cgttcccccg
     7261 ctccgccatg cggatgcagc gcggccacac caccggcccc cgtccggtga agctgcccga
     7321 gggctggctc gacgaccccg acgcgctcga accgcccagc cggctcgccg acctggccgt
     7381 ctccggcggc tgagggtggg cgccaccgtc gtgctggcgt cgggatcgcc ccggcgcctg
     7441 gagctgctcc gccgcatcgg cgtcgaaccg gtcgtccgcc ccgccgacat cgacgagacg
     7501 ccgggcccgg acgagtcccc cgccgccacc gtcgcgcggc tcgcccgcac caaggcgcac
     7561 accgtcgagc acaccccgga cgacctggtg gtcgccgccg acaccgaggt cgtgctccgc
     7621 gacgccgtcc tcggcaagcc ggccgatgcg gacgcggccg ccgccatgct gcgctcgctg
     7681 tcgggtcgcg cccaccgagt cgtcaccggc gtgcacgtcg tgtgcgggga gagggaggcc
     7741 gccgccgtgg aggagacgat cgtccacgtg cgggagctga ccgacgcgga gatcgatgcc
     7801 tacgtggcca ccggcgagcc gttcggcaag gccggcgcct acgccatcca gggcgcgggc
     7861 gggatgttcg tcgagcgcat cgagggcagc gacagcaacg tgatcgggct accgctggcc
     7921 acggtggtgc gcctcgccgg cgacgtcggc gtcaccatcc tgccccgctg acgcatcacc
     7981 agcactgcca gttcgagagg ggggtcggtt caggagacgc cgaccggttc gcggacgtcg
     8041 tcggactcgt cgccccggtc cgcgtcgacc cgggcggctg cggtggcgag ctgctgctcg
     8101 tggtcggggg caccctcgac gtgcagcgcc ggcaggagcc ggtcgatcca cctcgggagc
     8161 caccagttgc gctcgcccag cagctccatc gtggcgggaa ccaggagcat gcgcacgagg
     8221 gtggcgtcga ggagcacggc catgccgagg ccgaggccga acaccttgat gatgcggctg
     8281 tcctcgagca ggaagctgcc gaagacgacg accatgatcg cggcagcggc gctgatgacc
     8341 cgggcggtgg cggccagccc gtcggcgacc gagttccggg cgtcgccggt gcgctcgtac
     8401 tcctccttga tgcgggagag gaggaagacc tcgtagtcca tggacaggcc gaagacgatg
     8461 gcgaacatca tcatcgggat gaacggctcg atcggcgcgg gctcgacccc ggtgaggtcg
     8521 cccagccacc cccactggaa gatggcgacg acgatgccgt aggcgccggc gatcgagagc
     8581 atgttgagga tcacggcctt cagcggcacg agcagcgagc ggaacacggc catgagcagc
     8641 acgaacgaca tggccagcac cacgccgaag aagaggaagc tgcgcccggc caggtagtcg
     8701 gagaagtcga ccgacaccgc gacgaagccg gtcacgtgga cgtcggggcc gccgtcgccg
     8761 acgacggacg ggatgacctc gccgcgcagc cggttgatca ggtcctcggt ggcctcgtcc
     8821 tgcggcgcgc tggtgggcac gacgcgagcg agggcggcga ccggctgctc gaagtcgttc
     8881 ggcaacgccg gcgtgaccga ggcgaccccg gggtcggacg acaccgcctc gacgacctgc
     8941 tggaaggttg cgaagccctc ggggccgtcg acctcggcga cgacgacgaa gggaccgttg
     9001 aagccagggc cgaagccgtc gctcagcagg tcgtaggcct ggcgggtcgt ggtctcctcg
     9061 gggtagttgc cctcgtcgga gaacccgagg cggatgccga gcagcggcag ggcgagcacc
     9121 agcagcagga gcgtgccgcc gatggcgaag ggccacggcc ggcgctggat gaggtggctc
     9181 cagcggtacc agccggtctc ccggaccggc ttcggcgtgc ggcggggcac ctcgcggcgg
     9241 agcagcggga ggaaggtgcc cacgacgagg accacgatcg cgagcgggac gccgaggagg
     9301 agcatcggga tgccgagccc ggcaccgagg agggcgaccg agacgcaggc ggcggcgacc
     9361 aggccccgcc agcgggtcat ctccaccttc agctgcgcga agcccagcag ggcgggcagc
     9421 agggtggtgg acgcgaccat cgtcaccgcc acggtgatgg cggcggcgag ccccatcccg
     9481 gccacgaact ccagcccgat gagcaggagg ccgagcaggg agacgacgac ggtgatgccg
     9541 gcgaagacga ccgcccgtcc ggcggtgtcg atggcggcca tggtggccac ctccgggtcg
     9601 tggtcgcggt gcaggtactc gcggaaccgg gtgacgatga acagcgcgta gtcgatgccg
     9661 acgccgaggc cgatcatggc gccgagctgc agcgcgaagt ccggcatggg cgtcacgttg
     9721 gagatcaggt tcgtcagcat gatgccgagg ccgacgccgg ccagggcgac cgcgatcggc
     9781 aggcccatgg cgagcacgga cccgaacgac acgatgagca ccaggacggc gaaggcgagg
     9841 ccgatcagct cggcctccgg gggctcgaac ccggccagca cctcgccgcc gacctcgatc
     9901 cgcacaccgt cgatctcgga ctcgatctcc tcggcccggt cggagatctc gacaccgatc
     9961 tcgccggact gggagaagcc gacctcctcg ccgacctcga cccgggcgaa cgcgacccgc
    10021 ccggcgagct cgccctgctc ggcgatctgg ccgaaggcct ccggcgcgta ggggctcgtg
    10081 acctccacga acgggatctc gtcggtctcg gcgaacagct cctccatggc cgcctgcacc
    10141 tcggggtcgg tgaccccctg gtcggcttgg aacacgatgg aaccggccgg gccggcaccg
    10201 agcccgtcga agtgctcctc gaggacctcg aagccgtcgc ggctctcgga ctccggtgct
    10261 tcggggttgc cggtgaagtc cgaccccacg atggcgacgg cgacgccggt gagcaccacg
    10321 gccgcgatcc agacgacgac gaccggtcgg cggtgcgcgt agcaccaccg ggcgagctgc
    10381 atgtacacgg cgttcctcct gtggaccggc cccgggaggt tatgggcggt cgggtgacac
    10441 cttcacactc gcggcggacg agcgggatgt gacgtccgtc ccgttcgcgc ccttcgtgga
    10501 ccgctcgtgg tcgagcaacc actccttgcg atccgggcct ccgccgtagc cgccgacgct
    10561 cccgtcgctg cggaccaccc ggtggcaggg cacgaccagc cagatcgggt tcgccccgtt
    10621 ggcggtgccc acggcccgca cggcagcagg cgatccgatg gccgccgcga tctgggcgta
    10681 gctgcgggtc tcccccgcgg ggatggcgcg gagggcatcc cacacctgct gctggaacgg
    10741 ggtgccggtg gcgtcgaccg cgatgtcgtc gacggcggcg aggtcgccgt cgaggtagcg
    10801 ccgcacggca tcggctgcgg ccgtctcccc ctcgacccac tcggcgtggg cgaagcggac
    10861 ccgcaccttc gccgccacgc ggacgaagtg gtcggcgaag gcgagggcca ccacccgctc
    10921 gccgtcgtcg tcgaccgcga ccgccaggcg gaccggcccg gtcggggtct cgatggaggt
    10981 ggtgcgcacc gtggtgctgg tcatggtcgt tcctctcgtg gggtctgcca caggtgcatg
    11041 gcggcgtagg cccgccaggg tcgccacggc tcggcccggg cgatggcggt ccggtcgttc
    11101 gtcgcgtcga gcatccggcg gaggtgcagg tcgccggccg ggaaggcatc aggctcgccg
    11161 aggcggagcg cgacgtactc cgccgaccac ggaccgatgc ccttcagcgc gaccagggag
    11221 gcgacgagct cctcgtgggg gacgctgccg tcgaggacca cggcgccggt gaggaccgat
    11281 tcggccaacc ggcggatggt gtcggcgcgc tggccgatga tcccgagctg ctccagcggc
    11341 gcaccggcca ggtcctcggg ctcggggaac gcgccgtccc ggccgtgggc cgcgacgatg
    11401 cgcccgagca cggtgcgggc cgcggcgacc gagatctgct ggccgatcac ggcccgcacg
    11461 ccggcctcga acccgcccca gctccccggc acccggatgc ccggtcggcg ggcgacgagc
    11521 gggccgagga gcggatcggc gccgaggtgc tcgtcgaccg ccaccgggtc ggctccgagg
    11581 tcgaacgccc ggcgggcgcc ggccacgagg gagccgaggt gggtgtcggg cggcagatcg
    11641 acgccgagca cgaccgcgtc ggcgcccggg cggatcgtga ccgagccggt ccggtccccc
    11701 accgacaccc gccgcgcgta cgtctcggcg tccacgacct cgaggccggc gacggcgcga
    11761 gcggcgaacc agtcgaggag ggcggcgccg tcgaacggcg gtcggaccgc gaggcggagg
    11821 gtcagggcgc acgacgcaac accagcactg ccagttcggg agggggtccg cccggtgggg
    11881 cgcaggtcgg cgcgcagctc ggtcggggtg gcgcggaacg tggccttgag cacgtcgttg
    11941 cactggcgca cgctgccgaa gccggcggcg aaggcgatgt cggccacccg caggtcggtg
    12001 tcggcgagca ggcgccgggc caggtgggct cgtcggctgc gggcgacggc gtcgggggtg
    12061 gtcccgaggt gctcgacgaa gaggcggcgg aggtggcgat cgccgacgtg cagcctgcgg
    12121 gccaaggcgg cgacgtcgcc gtcgtcaagg gcgccctcgt cgatgagccg cacggcccgg
    12181 tcgacgaccc cggccgcgcc cttccacccg ctggacccgg gcgcggcctc cgggcggcag
    12241 cgccggcacg gtcgcagccc ggcgtcctcc gcggcagccg ggtgggcgaa gaagtcgacg
    12301 ttccgggaca gcggcgtgcg ggacgggcac accgggcggc agtacacgcc ggtggtgcgc
    12361 acgccgaccc agaaccgccc gtcgaagcgg gcgtctcggc tgcgcagcgc ctcgtagcac
    12421 tgctggtggt ccaggctcac gagacgatcc tgccccatcc ccgccgatgc gtctggcggg
    12481 atccggacat gggagtgccg gtcgtgggcg gcgcgggccg tggctacggt gcgaccgtga
    12541 ccgatcccgt gcagctgacc atcgaggacg gcgtggccgt cgtccgcctc gacgacggca
    12601 aggccaacgc cgtctcccac acgctcatcg accaggtgca cgccgcgctc gacactgccc
    12661 aggccgacgc gaccgccgtg gcgttcatcg ggcgggaggg gaagttctcc gccggcttcg
    12721 acctgtcggt catgacccag ggcgccgacg cgacccgtgg cctggtgaag gccggcggcg
    12781 agctgctcat gcgggtctac acccaccccc agccgaccgt cgcggcggtc accgggcacg
    12841 cgctcgccgc cggtgcgctg ctctgcctgg ccagcgacac gcgcatcggg tcggcagacc
    12901 tgcccgccaa gatcgggctg aacgagaccg ccatcggcat ggggctgccc tggtacggca
    12961 tccgcctcgg ggaggcccgc ctctcgctgc gccaccgcca gcggtccgtg ctgcaggccg
    13021 agatctacga catggccggt gccctcgacg ccggctacct ggacgagctg gtggacgccg
    13081 acgccgtggt ggacaccgcg atcgagcagg cccgccggct cgggcagctc cccggccacg
    13141 cctacgcgtt cacgaagcga cgcctgcgcc aggcgctggc cgacgaggtc ctgctcggcc
    13201 tcgacgccga catggagacg atggtcccgc ccggcagctg agcggtcgcc gccgttccgt
    13261 ggccgctgcc agttcctcct ccacctggaa ctggcagcga ctgcggaaca gtcgggtcag
    13321 cgctcgtcgc ggcgcagcac gtagagggcc gtggtgccga tccccttcag gtcgcggggc
    13381 cggatggacc ggaaccgcag gtcgtcgacc tcgccgatgc ggtcgacgaa catctggtcc
    13441 acgaggaccg aaccgggacg ggcgtaggag acgagtcggc tggccaggtt cacgggcggg
    13501 ccgaacaggt cgccgagccg ggacagcact gagccgtgcg tgagcccgac ccgcaggtcc
    13561 ggctggtcgc cgccgaagga ctcgaccagc tccagggcga ggtgagcagc ggcttcggga
    13621 tcgtcgaaca cgaacagcac ggcgtcgccg atgagcttga cgacccgacc gccgcgctcg
    13681 gtgatcatgt cggccgtggt cgcctcgaac gtctcgagca gctcggccag gtcggcctcg
    13741 gccagctggc ctgacagccg ggtgaaggag accacgtcgg cgaagccgac cgtcacgtcg
    13801 ggctgttcga gatcgtggcc ggcgatgcgg cggatggcgc cggcgtagag gtggcgccgc
    13861 agcaggtatg agagctcctg ctcgatcagc gggttcacca gctgggcggc cagcaccatc
    13921 aggtcgtcgt tctccccggg cggcagggcg tccatctcgt cgatggcatg gtcgacgatg
    13981 gagtggagcg cgtcggcgac gcggctcatc gtctggccga gcagccgggc gaagcggatc
    14041 gccgtctccg acgagatgcc cgtcgcctgc aggaagtcgt tgatggcagc gagcgcggtg
    14101 acgtcggcgt ccgtgagcgc cacggcctcg tccggcacgt gggcgaagcc catcgcccgc
    14161 cacagcgctc gggcgagctc ttcgtcggcg ccggtgcgct gccaggcgtc gggtgggcgg
    14221 agccgaggtg gctccccgag cagcaccacc gcctgcgcca accgccgcag ctgctcggga
    14281 ccgtcgacct ccgcggcggc cacgagctcg ggatcgaccc cgagggcgtc gcgcaggtgc
    14341 gcagcgaggt cgaagggcgg gaggtcctga ccggtcacgg caggctcact cggccacggt
    14401 gcaggcgatg acgaccccgg cctcggcctg gcactcggcg atcgtcgccg ggtcgagggc
    14461 gaagctgaag gcacggtcgc cgatctcgag cagcgccggc gtcccggcgt cgggatcggg
    14521 ctcgaccggc gcccacggct cgaagacggc gatggcgttg gccgatgcca cgttgcgggc
    14581 accgatgggg tcgccggcca cgagcgcgtc gaagaacgcc tgcgccgcac cggcggtgtc
    14641 cccaccgccc tgggcggcac ccacccactc ggcctggcac tggtcgcgtg cgatcgcctg
    14701 caggtcgctg tcggcggcga gcacccgtcc ggtccggtcg tcctcggcga tgatggcggc
    14761 cagctcgtcg acctcggcgt cgtcggcgga ttcgaccacg gtgtcgaggt gctcggggct
    14821 cggctcggcc aggtactcca ggtaggcgtc gcagaaccgg gggccgcgct cggagcgctc
    14881 gtcgcgctcc tcggtcccgt cctccggggc cgccccgtcg tcgccggcgt cgtcgtcacc
    14941 gcacccggca gcgaggagcg aaccgacgag cacgagcgcg agggcgagtc ggcggtggcg
    15001 gaggcgtcgg tgcacggtcg gcaccctagg gaaaccggcc cgatcagcca tcgacggatg
    15061 tcaggccgcg ggacacggcc tcgtgcagcg ctgggcgcag gcgcgccggc tcgatgatct
    15121 cgtgcaccga accgacgtcg cgggcccgct cgacgctgtg gacccgatcg aactcggcag
    15181 ccacctcgcc gaccatctcg ttgcgcaccg acgagcgcac ggcatcgagc tcggctcgca
    15241 gcctggcccg ttcctggggc ggtgcggtgg ccaggcgctg ctcgagctcg gcgacccgcg
    15301 catcggcctg ggttcgggcg tcgacctccc gcgtgaacac gacggccgcc gcaggcgcgc
    15361 cgccgatgac cgacgcgtgc gaaccctcca cggcgagcac ctgcatgcgg tcgttgagcc
    15421 gcccggagaa cacgacgaag gcgccgccgt ggtagcggga gatcacgcac aggacgatcg
    15481 gtccgtcgaa gttcacgatc gcccggccga tctcggcccc gtactcgagc tgcagccggc
    15541 gcagggactc gggcgagccg tcgaacccgg acaggttggc gagcaccacg acggggcggt
    15601 tcccgctcgc cgcgttgatg gcccgggcca ccttcttcga cgacaggggg aacagcgtgc
    15661 ccgccgacca ctggctcggc ccgtcggccg gcagctggcc ggtgcgcggc agcggacggg
    15721 actcgatgcc gaccatgcag atcggcaggc ccccgaggtg ggcgtcgagc acgacggcgc
    15781 cctcggcctc ggccatgtcg gcccagcgct cgagcggctc gaggtcgtgg tcgatcaccg
    15841 cgcgcatcac cgagcggatg tcgaagggct tcttgcgccc cgggttggtg gcgtcggaga
    15901 agatctcgcc gaccgtggtg aacgtggtcc cggcgccggc gtggggttcg tcggtgacgt
    15961 cgcggtcgat ggggtcggtg gtgagcgtcg gacgaggacc gggctcaccg ggggccacgt
    16021 agctgtggtc gtagtgggcg aagaggatgt cgacggcacc gaccatgtcc ggcgcccagt
    16081 actgggcctg gccgttgggt cccatgaccc gctcgtggcc gccgatgccg aggttgtcct
    16141 cggccgacac gccgccgctg aagtcgatgg cctgcttgcc ggtgaggacc atcgtcgagt
    16201 ccggggtcat cacgaggatg cccttggtgt gcatcagcat cgtcgactcg gcgttccagt
    16261 acggctgggc gccgacgttc acgccggcca cgacgacgtt gatctcgccg cccgcctggg
    16321 tgaactccac gatccggcgc agcaccgagg cgacccagtc caggttctcg ctgccgctgt
    16381 ccatggcgat gcgggcgccg gccgagaggg cgaaccactc gacgggcagt ccccgctgct
    16441 cggccaggtc gatggcggcg agcagccggc ggcactcggc ctcggcgatg gagcccatcg
    16501 agcgggtcgg atcgccgagg atcgccaccc gggtcatgcc ctcggggtgg acctcggtgg
    16561 cggcggtgac gaggccgacg acgacgccgg cgacgttgtg cccgggcggt cggtcgacgg
    16621 gcacgaggcg gtcgccgtcc aggtcgagct cggtgaagtg gccctcgccg tgctcggcct
    16681 cggtggtgag catcgggacg atctcgtacg ggtacaccgt gccccggcgc cggcaggcca
    16741 gcaccttctg ggtgtagtcg tccagcggct gcatcggggc cgtcggcggc tccgtgacct
    16801 gcatcgtgag cccccgcccg gcctggaacc cgaggcggaa cacgacgtcg gcggtgtcgc
    16861 cggatccggg gtcggtcacc cggccctgca ccagcacctg ctcgatgccg agaccgttgg
    16921 tgaggggcac cagccgcttg gccacctcgg tgacctcctc gaggctgatc tccacggtgg
    16981 gccagacgaa gagcatcacc cggttccact cgaggcgccg gcgacggcgg tcggtggaga
    17041 gcgagcgccg gatgccgtcg aggcacgaca ccaggacctg ctcgagctcg gggagggcga
    17101 tgagcgcccc gctggcgtcg cggaccgggg tgatgtcacg cacctcggcc acggccacca
    17161 gccgctcgtc tccgggctgc tccaccgaca cgcagtcgaa gacgtggacg tcctcgggac
    17221 cggggacgcg ggccacctcg aagttctcga gccgccacag ctgcaggcgc tggccgatca
    17281 tcgggtgcag gccacggaac accgtgacct cgcggaacga gccttcggcg gttcggtccc
    17341 aggacaggtg gtggacctcg ccatggtcgg ccccggcggc cgcggcgacg atcgcgatgc
    17401 gctcgaccgc gcccgggatg tccgcggcgg ccacggcctc gtcgacccgt gccaccaggt
    17461 cgtcggcggt gcgcggctcg gcaaggcgca cgtagaggtc gacgacgtgg gtcgcatcgc
    17521 ccccggcgcg gcgggtcacg tcggcgacgg cgtcgcccag cgtggcctcg tcgcaggcga
    17581 caccgatgac ctcgacccga cgaccccggt gctcgtaacc ggcggtcacg gcgggcaggg
    17641 cgccgaggtg gcgctcgagg ccctcgaggt cccggatctt gtagaagcga cgggtgagca
    17701 cctcgacgat ggcggccgag agcgcggcgt cgcgcccgag cgcgtcctcg ccggcgatca
    17761 ccgacatcag cggcaccggt gtcgccacca gccggtcgga ggcggcacgg tcgccgggat
    17821 cgtcggccag ggcccgcagc gaccggcgca cctcatcgtg tacatcggct ctggcgtggt
    17881 cgagcagcgg ccgatcgatg cagcggtggc ggacgctgcg cgccaggttg ccgatggctg
    17941 ggtactgcgc ctgggtggcg gcgatcagcc ggtcgagggt gtcgcgcacc gcgtcgcgct
    18001 cctcggggtc gccgcagtgg tcgaggcggc gctcgagcag cgcctgcacc acgggcagct
    18061 gctcctgccg gcgctgcacg gcccggtgga tgctgtgcac cgcggcttcg agggcggtcg
    18121 tgcggtcgag ggagcggacg ccgtagtggg cgagcgcgtc ctgcagccgg gtgcggaacc
    18181 gctcggggag gctctcgtgg tcggcgtcca gcgtgcgcag gtaggcgtgg aagtgctcga
    18241 gcgggctgcg cagctcgagg ccgtggtcgc cctcggaccc gcgccggtcc cgggtgaggc
    18301 cgcagaggtc ggcgaacacg cggagcagct cgagctcgcg ccggcgcacg gtggggtcgg
    18361 cggcatcggc ctcgtcgcgg tgggcctcga tgatccggtc gtcccggtcg gccacgtcga
    18421 acccgagcag gagcgagcgc agcacgtcca ggcggctcgt ggtccggtcg gtctccgagg
    18481 ctctacccgc cggcgcgatg cgaagtcgct ggcgcgtgcc ctcgtcgtgc tcgccgttcc
    18541 cgtcgctggc gccgatgcgc agcagcgggg cgccgacgtc gacctggacg ttgggggaga
    18601 cgaagacctc ctcgaccacc ccgtcgctcg gcgcgaccac ggccgtctcc atcttcatcg
    18661 cctcgaccac cgccacccgc tggccggcga cgacctggtc cccgggccgc acctcgaccg
    18721 agaccaccag ggccacggcg ggtgcccgga ggagaccgcc gtcgtcccgg gagaagcggt
    18781 ggctcacgcc ctcgacctcg acgaggtgct cggtgcggtg ggcgtgggag accacccggt
    18841 aggtgcggcc gccgacgacg aggcggctgt gggcgtcgtc gaggcggtcg agcaccacgt
    18901 cggcgacgac gccgtccatc gtcacccggt accagccggt gaggtcgccg gtggccacgt
    18961 gcaaccggta ctcgtcctgc tcccagcgca gctccacgtc gtggccggcc tcgaggtcgg
    19021 attcggggcg gccgcgcgcc gccgagctga ggaagcgggc ccggtcgagc aggcgctcgt
    19081 ggtcgtggac gtcgatggcg gcgacgagca gggcgatctc ggcgaagcgg tcggtgcggt
    19141 gcccgtcgga accggtgagg cggtcgagcc aggcggtgtc gacgtcgccg acgacgacct
    19201 cgggacggtc gacgaggtcg agcaggaacg acttgttggt ggtgccgccc tcgaccagca
    19261 cggtggtctg ggagagcgcc cggcggaggc ggcggcgggc ctgctcccga tcggcacccc
    19321 aggcgatgac cttggcgatc atcgagtcgt actcggcggc gatcacgtcg ccctcggcga
    19381 cgccggtgtc gacccgcacg ccggggccgg tgggcaggac caggcgttcg atgcgcccgg
    19441 cggcgggggc gaagccgcgc tgggggtcct cggcgttcag gcgggcctcg aaggcgtgcc
    19501 cgttcggctc cgggggttcg ccgaccaggg ggtgcccgag ggcgacctcg atctgcagtc
    19561 gcaccaggtc cagcccggtg gtgagctcgg tgacggggtg ctcgacctgc aggcgcgtgt
    19621 tgacctcgag gaaggcgagc aggtcctcct cgggctggta caggaactcg acggtgccgg
    19681 cgttgacgta gcccgacgcc ttggccaggc gcaccgcggc gtcgcgcagg cgggcttcgt
    19741 gcccgggccc gagggcggtg gaggcggact cctcgatcac cttctggttg cggcgctgga
    19801 tgctgcagtc gcgcacgccg ggcgcccaga cgttgccgtg ctggtcggcg atgatctgga
    19861 cctcgacgtg gcgggcgtcg ctgacgaccc gctcgaggaa gaccgtcggg tccccgaacg
    19921 acttcgcacc ctcgtcccgg gcccgctcga acgccgaggc cagctcggcg gggtcgtcga
    19981 cgcggcggat gccgcgaccc ccgccaccgg cggccgcctt gaccatgagc gggtagccga
    20041 cgcgctcgcc cgcggccacg gcttcgtcca cgtcggcgac cgggccgccg ctccacgggg
    20101 ccagcgggac gccggcctcc tccgcgaggc gcttcgcctc gatcttgtcg ccgagcaggc
    20161 gcatgcactc ggcgctcggg ccgatgaagg cgacgccgag ctcggcgcag cgctcggcga
    20221 actcgggccg ctcggccacg aagccccacc cgacccatgc cgcctcggcc cgggctgctg
    20281 tgagggccgc ggccagcacg tcgaggtcca ggtagggcgt gccggcgatc gggacgccga
    20341 ggtcctcgaa gcagaccgcc tcgtcggcct cgcgcacgaa catcgcccgc cgttcggccc
    20401 gggtgtgcag ggcgatggtg gtgaaggagc cgtcgagctc gcggaccgcg tgcacgaggc
    20461 gcatcgcggc ctcaccccgg ttgacgatcg cgatgcgccg gaagtcgacc acggacgtca
    20521 gaccgtcccg gcgcgagccg gttacacccc cggctcagcg ggccgggtcg cctcgctggg
    20581 tcacgtgccg acagcccggg tcggcgtcga tggcctcgtc caggccggcg gccacgtagg
    20641 ccgagcgcgg gggctccagc tccgagaacg ccttctccag ggcgttctgc agctcgccgg
    20701 cccgcccgtg ctcgtcggcc tgggtgacgg cccggacctc ggcgacgaac cggtcccgct
    20761 cgcgctccag ctcggcgacg gccgaccgct ggccggccgc gaaccgctcg gtcagctcgg
    20821 gctccccgtc cacgaccacc gggtcctgcg ccgccagggt ctcgaccagg cggtcgtatc
    20881 cgtcggccag cgcggcggcg cgggtctcgt cgtcctcggc ccgggccagc tcggacaggg
    20941 cctcgttggc cacctggatc agctcgttct cccgctggcg gagctccgcg cacaccgcca
    21001 cctcctccgc ggccgcgggc tcggcgtcgt cgtcgccgca cccggcgagg accgtcgcca
    21061 ggacgacggc cccggccacc cctgtcccga accggacgcg gatccgacgc accggacgct
    21121 cagctcggcc agtcatcggg cggctccgtg gcccggggct cgagcggcgg gtcccaggcg
    21181 ccgtcgtgcc agcggcgcac cacccggccg ggcacgccgg cgacgacgct gcggtcgggg
    21241 atgtcgccgc gcaccacgga tccggccgcc acggtgacgt gctcgccgat ggtggtgccc
    21301 gggaggatga cgacgccggc gccgatccag gagccgctgc cgatggacac cggcgcctcg
    21361 tggggccact gccgggcgat cgggacctcg acgtcgtcgt aggcgtggtt gtggtcggtg
    21421 acgtagaggt cgggcgcgat ggtcacgtcg tcgccgatcg cgatgccgat ccgggcgacg
    21481 agcgagccgc cccggccgat gttgcagcgg tccccgatgc ggatcaccgg cggcgcgctg
    21541 gggtcgaggt cctcgttcca catcccgacc gacatcgaca cgtgcgggcc gatgagggtg
    21601 aaggcgccga tctcgatcca gtgctcgttg aacagctgcc cgggcgggaa cgcgatcatc
    21661 gaccgctccc ccatggagcg gaaccgccgg ccgtgggcgt cgtcgggcga ggtggcgccg
    21721 accgcgcaga tccactccca gctgcgctgg atggcgccgg cggcgaggtt gcgcacccgc
    21781 ggcgacagct ctccgggctg gaacacgacc gggacgctag ccagccacac tgggcgggtg
    21841 gcccgccgac tcgtcgactt cctcccccgc cccaccggcg gtgagcccga ccccgacgcc
    21901 atcatcgagg cgttcaccga ctgggccatc gagcggggcc tcgagctcta cccgcaccag
    21961 gaggaggcga tcctcgagct gctgtccggg tcgaacgtca tcctcgccac gccgaccggg
    22021 tcggggaagt cgctggtcgc cctggctgcg cacgccaagt cggtggccgg cggcgggcgc
    22081 tcctggtaca cggcgccggt gaaggcgctg gtgtcggaga agttcttcga cctgtgcgag
    22141 cagctcggcc cggagaacgt cggcctgctc accggcgact cctcggtcaa cgccgacgcg
    22201 ccggtgatct gctgcaccac cgagatcctc gcccacgtcg cgctgaagga cggcgtggag
    22261 gccgacgccg agaccgtcgt gctcgacgag ttccacttct acggcgaccc cgaccgcggc
    22321 tgggcctggc aggtgccgct gctcgagctg ccccagtcct ccttcctgct gctgtcggcc
    22381 accctcggcg acgtgcggtt cttccgcgag gacctgaccc gccgcaccgg ccgccccacc
    22441 gcactggtcg acgacgccgt gcgcccggtg cccctgcact tcgagtaccg gcgaacgctg
    22501 ctgcaccaca ccatcgagga gctgctggcc aaggacgctg cgccgctcta cctcgtgaac
    22561 ttctcccaga aggacgccgt cgcccaggcc acggcgatgg ccagcgtcac caagctcgac
    22621 gacgacgcca aggagatcgt caccgccgag ctgaccaagg ccaagctgcc cgccggcttc
    22681 gggcgcgacc tggcccgtct gctccgccag ggcgtcggcg tgcaccacgc cggcatgctc
    22741 ccccgctacc ggcgcctcgt cgagcggctc acccagatgg gcgtgctcaa ggtcatctcc
    22801 ggcacggaca cgctcggcgt cggcgtgaac ctgccgatcc gcaccgtcgt gctcacccgc
    22861 ctctacaagt acgacggcac cgacacccgg gtgctcaccg cccgcgagtt ccaccagatc
    22921 gccgggcgtg cgggccgggc gggttacgac accgccggcc tggtcatcgc gctggccccg
    22981 gagcacgtgg ccgagaacga ggtcgcccag gccaaggccg ccgacgaccc caagaagaag
    23041 aagaagctga agaagtccca gccgccgaag ggcttcgtcc acgacgacga ggagaccttc
    23101 accacgctgc aggaggccaa gcccgaaccg ctcgagtcct cgttccgcat gacgccgggg
    23161 atgctgaggc agctgctcga ccgacccggc gacgcgtggg cccacggtcg ctcgctgctg
    23221 ctcgacagcc acgagccccg gtcgcgccag cgccgccacg tgcgctcgac catcgggctg
    23281 tacaaggcgc tgcgcaccgc cggcgtcgtg cggctgctcg acgagccgga caagtacggc
    23341 cggttcgtgg aggtcgacca cgagctgcag gacgacttcg ccctcaacca gctgctcgcc
    23401 ccgttcctgc tccatgccgt gccgctgcgc gaccgcgacg acgagggcta cccctacgcc
    23461 gtgctcgcgc tggtcgaggc cgtggtcgac aacccgtttc cgatcctcat ggcccagaag
    23521 gacaagctca aggacgaggc catggccgag atgaaggccg ccggcgtcga gtacgagcag
    23581 cgcatcgagg agctggacaa gatccagtac ccgcagccga tgcgggagca gatctacgac
    23641 gtgttcgaca tctggcgggt ggccaacccg tggatcggcg accgcaacat cgcacccaag
    23701 ggcgtcgtgc gcgacctgtg ggaccgggcc atggacttcc ccgccttcgt gcgccactac
    23761 ggcatcaagc gctccgaggg cctgctgctg cgctacctgt ccgacgtcta caagacgatg
    23821 ctgcgcaccg tgcccgacga ggccaagacc cccgaggtcg tcgagctgca ggactggctg
    23881 ggcgccgtca tccgggccac cgactcctcg ctcctcgacg agtggaccgc catgctcgcc
    23941 ggcggcgacg ccgacccggc atcgctcgcc cgcgaggtcg ccgaggaccc cggcatcccc
    24001 gacgtcacca ccgaccgcca cgcgttccgg gcgctggtcc gcacccgcat cttccgctgg
    24061 gtgcagtacg ccgccaccgg ccagtggact gcgtggtccg aggacctcac cgaggtcggc
    24121 gaccggggct ggaacgagcg caagctgctc gagcagttcg cgccctacct cgaggcctac
    24181 ggcgacgacg ccccccaacc cgacccggat gcccccaccg cccgccccgg cgaggagcgc
    24241 ccggccgtcg tcccccgccc ccgcatcggc atcgacggcg acgcccgcgg cccggctcgc
    24301 ttcgtggtcg ccgacgaggg cgacgagtgg gagatcgagc aggtcctcgt cgacccggag
    24361 ggccacgacg agtggtacct ggccgtcacg atcgacctgc ccgcctccgc cgagcagggc
    24421 gacgtcgtcg cccacctcga gggcctccgc cgccgataga ccccggcgaa tctcgtacct
    24481 gggggacagc ggctgtcacc gagccacgac agacgcccgg cgcacggcct cgatggctcg
    24541 cacgaggtcg gcggtgaacg tcgccacgcg gatccgttcg accgtccatc cgaccgcctc
    24601 gagcgcgcct tggcggatcg catccgcgtc ctgcgccggt ttctcctcgt gctgggagcc
    24661 gtcgatctcg atcgccacca tcaggtccgg gaacgccagg tccaggcgat atcggttggg
    24721 gccaacctcg acccagtgct gggcgaccgg tcggaggccg gcgtcgagga acgcgtccag
    24781 caccacctgt tcgaggccgg actccgggac cccaccgacg tcgacgtagc ggtccagcca
    24841 gtcccggagt gccgcaccac cgttccgacc cgaccgcttg tgccggaggt aggtccgcag
    24901 cagcgcaggg aactgcacac cgtgtgacgc ccggagcccg cgcatcgcct gtcggtactg
    24961 agacggcgac accaccgaac cgagatccat cgcgagacga cgaggtccgg tgatcgggat
    25021 gccgtcgatg cgtcggcggt cagcgagctc gaggtccgtc gattcgtgca cgacgagcca
    25081 cggccgacgg agcgtcgtcc ctcgggggat ggtgacctcc gggctcggtc gggtgaaccc
    25141 accgacgccc agcaacgcag cgctcgagcg gtgcgagccg agcgagccat cgggtgccag
    25201 cagcagggcg agcatcagcc gcctctgcca ggtcagcgcg acgcccgtgg ggccgtacac
    25261 cccgaggtcg aggcgctccc agaggccgcg gtcgaccagg cgttgacaac gcttcctccc
    25321 gatgccgacg ccctcggcct gggatgtggt gatgaggctc tgctggtgag cgaggatgcg
    25381 gccggcggcg acgagctcgg cggacacggc ttccctccgt tcggaacgga cgtgggggaa
    25441 gccagggcga tcgtaggcgg gcttgcgtgg gtgatgcgtc tgtcgtggct gggggacatc
    25501 cctcgtctcc cagccacgag attctcggta agggtggagg gatggctcgc ctgctgatcg
    25561 tccaccacac gccgtcgcca tcgctgcaca cgatgctggg cgcgatccgg gacggcgcgg
    25621 gcgccgacgg gctcgaggag gtcgaggtgg tcaccgcggc ggcgctgtcg tgcacggcgg
    25681 ccgacgtgct cgccgccgac ggctacgtgc tgctcacgcc ggcgaacctc gggacgatgg
    25741 cgggcgcgct gaagcacttc ttcgacacca tctactaccc gtgcctggag gagacgcagg
    25801 gccgcccgtt cggctgcgcg gtccacggca acagcgacgt cgacggcgcg ctgcgcgacg
    25861 tcaccaagat caccaccggg ctcgggtgga gccaggccac cgaccacgtg cgggtgctcg
    25921 acacgccgga ggccgaggcc cgggacgcgt gctggaacct cggggccacc gtggccgcca
    25981 cggtgctgga gacctgatcc ggccccgagg tgggcggcgg gggccgaccc gtcggaccgg
    26041 ccccctgtgc gccgcgccta gctcacggtg aactcggcga tcatcccctg ggtgaagtgg
    26101 ggcgggccct cggactccgc ttcggcgtcg ggtgtcgacc ccacggggat gaagcagatg
    26161 gcggcgtact cgccgggttc ggacatgtcg aacaacccgc cggactcacc accgggcggc
    26221 gcgaacgtcg caccgatctc ctggaccagc gccatggccg cctcttcgtc cagcgcgagg
    26281 atgtcctcga aggactccgt ggtgtcgtcg cccttgcgga gcacgatcat ctcgtggaac
    26341 tcgtctccct cgttggagaa gtccacgccg tagacaccta ccgacagctc ggcgtcgatg
    26401 ccctcgtacc cgtagtcgat cgcggtgacc gcgatgacct cgtcggcgca ctccttgttg
    26461 gccacggacc cgacctcgct gaacgcggcg ccgaactcct cggtgtcgaa gaacccctcg
    26521 gggtcgtcct cgaacccggc ggcgagcgtc tccaacggtt ccactgcgcc gtccggcgcg
    26581 atgccggcga cctcacggat cgcgtccggc gtcggatctc cggccagcag ctcctggtac
    26641 gccgagcaga actcgccgtc gccggccgcc tgctccccct cacccgacgc cgtggtgtcg
    26701 tcggtctcgg cggtgtcctc cgtctccgcc gtggtgtcgt cggtctcggc cgacgtgtcg
    26761 ccttccgtca cctcgtcgtc gtcgtcaccg cacgcggtga tgacgagcgc gagcgcgacg
    26821 aacgcggcga tcgcacgctt ctgcatcgtt cccccacact tctgcccggg accccagtcc
    26881 cacccagtgc cggccgtccg accggctcgc ggatcgtact gcgatcggcg cagaggggat
    26941 agcggaatct cagggggcgt cggtggtgct cgtggtgtcg tcggcctcgc tggccgagcc
    27001 ggtgtcctcg gtctcggact cgacgccgga cgccgaaccg ctgccggagc cggagccgga
    27061 acccgatccg ctgcccgagc cggagccctc ggtctcgccg atctcccgga cgtcggcgcc
    27121 gtcgtcgtcg ccgcaggcgc tgaggccggc ggcaccaccg aggacgaggg cgagggccag
    27181 ggcgaacgag gacgtgcggc ggagggcgga gcgcatggcg gccacgttaa ggattcctaa
    27241 cggtcggagg caagcaccgg cccgacgcgt tccggtttcg gtcggagcga ggtcgctcgc
    27301 taccgtgggc gtcgctgctc tctgggccaa cggcgtccca tgttgcagag cctgaacccc
    27361 agttcgactc tccacgggac cccaacatga acgatctccc tgcgaagcag ggtctctacg
    27421 accccgcctt cgag
//
LOCUS       DATISU010000028         7275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_373843, whole genome shotgun sequence.
ACCESSION   DATISU010000028 DATISU010000000
VERSION     DATISU010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373843"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VLR27_01460"
     CDS             complement(<1..769)
                     /locus_tag="VLR27_01460"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP02137.1"
                     /translation="MALDPAISARVAHLWDAHVLQLLSGEVEGVPPAGPMVVDGVVRT
                     DGVVPGPGGPIPVRWYRSTALATDELAPTLVWAHGGGWQFGDLDMPEADSVAQRVAAG
                     LPGVVVTVDYRLAPAHRHPAALEDVLAVQRWVVDHGAERGVDPHRVALGGASAGGHLA
                     AITALALAGEAAAPAALWLAYPVTDPDGGPYDGRHPDCPPVLWLDHEGTTKLFEGYLG
                     DDPAGADPELIPARRDLSVLPPTLVTTAECDSLCPQAR"
     gene            complement(850..2640)
                     /locus_tag="VLR27_01465"
     CDS             complement(850..2640)
                     /locus_tag="VLR27_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP02138.1"
                     /translation="MGWMGGGVDQDEQLDRDTAVHVLKRTYGFLRPYRRRLLAAALFA
                     VLYTASTLAGPFLVRYGIDEGIKPKDGDVVNRAVIAYVVVAVIAYVVYRRQVLAIARI
                     GEDFLRELRIRVFDHLQRLSMPFYDREKAGVVVSRMTSDIDSLSELVQMGLLMFVQNG
                     LLLVVSVVVLGVVSPQMLALCLVAVPGVVIASRKFQRESNEAYLDVRDGIGATLSELQ
                     EGISGVRVVQAFAREDVQTTRFETHNRQLYDAHMRSVKISAWYLPVIEIAGLGTTALA
                     VGIGGFWVHEGLLTIGTVTFFILTLSNLFEPIQQLSQLFNTVQAAGAGLQKLYTLLDT
                     GVDVPERPGAVDLPGRGAIEIAGVGFHYASDPDTTVLEGVDLRIEPGERLALVGPTGA
                     GKSTLAKLIVRFYDPTEGSVSIGGVDLRHATLASLRRTMVVVPQEGFLFNGTLMENVR
                     LARPDATDDDIVAAMDSIGILDRFADLPEGLHTEVRERGSRFSAGEKQLISLARAALA
                     DPEILVLDEATSSLDPGTEAIVEEAMERLSEGRTTIVIAHRLTTAARADRVAVVAAGS
                     LAEVGTHDELIAADGHYAALFAAWAGGQPV"
     gene            complement(2640..4343)
                     /locus_tag="VLR27_01470"
     CDS             complement(2640..4343)
                     /locus_tag="VLR27_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP02139.1"
                     /translation="MVGLFWSFSKVGVGVLLQRAIDNGIENDDMSSLAIWAVAIFVVG
                     LLSAVLTGSRRYLAFREARLVEKRLRDRLFAHVQRLHFAYHDEVQAGQLMSRGNIDLQ
                     HLQNFVVMIPLTIANGMTVLGVTVILLIIDPLLAVLSLAALPFINVVAKVFGSRLFPS
                     VMGIQRESAELASVVEESVAGVRVVKGFGAEDVVRGRLRRTADDVYRESMNASKIRSV
                     FLPALELLPNIGLITVLGVGGHRVINDQISLGSFVLFNFYVVLLIWPLRMLGMIIANG
                     QRASAAAQRVAEVLSTDPVIVDRPDASGLPPGRGEVRFEHVEFSYGDGSVPVLHDFDL
                     VVEAGTSVALVGATGCGKSTVGRLIPRFYEVDSGRVLLDGVDVRDVRVAELRRAIGIV
                     FEETFLFSDSIAANIAFAEPDASMDQIRTAARLAGADEFIESLEHGYDSVIGERGYSL
                     SGGQRQRIAIARAILADPRVLILDDATSSVDPTKEHEIRDALGEVMEGRTTIVIAHRS
                     ATIALADRVVLLDEGRVVATGTHDELLATNPRYREVLAAAALEELEATDVDPDSPEVR
                     A"
     gene            complement(4466..4942)
                     /locus_tag="VLR27_01475"
     CDS             complement(4466..4942)
                     /locus_tag="VLR27_01475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HSP02140.1"
                     /translation="MSAIDQATYRQVLGHFPTGVTVIAGMDGDEPVGLAVGSFFSLSL
                     DPALVGFCVDRSSGSWARMADSGSFSVNVLGADQEDVCRVFATSGDDKFDGIGWKPAE
                     SGSPLIDGVLAWIDCAIEDVHDGGDHEIVVGRVLGLDVANEGSPLIFYRGGYANLK"
     gene            complement(4954..5793)
                     /locus_tag="VLR27_01480"
     CDS             complement(4954..5793)
                     /locus_tag="VLR27_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSP02141.1"
                     /translation="MQVTFFGVRGSTPCPHDDNHRYGGNTSCAVIEEHGHDPIVLDLG
                     TGLRTWGDLLPHDGSFRGHALVTHVHWDHIQGLPFFAPVLQPGARLDVYGPPPELDGK
                     RTLDDCFDQFVCPPLFPVRLGDLPGDITFHTVRDTELAVGDAKVKVRDVPHTSVTNGY
                     RVELGGATVVYISDHQMPLDESLVVTDDVLELCAGADLLIHDSQYTSEEFAKKRDWGH
                     CTVEFAVHVAAEAGVRRLALFHHDPTHDDVFLDCLVEQARAIAGPRVEEVLAASEGLT
                     IVL"
     gene            5894..6784
                     /locus_tag="VLR27_01485"
     CDS             5894..6784
                     /locus_tag="VLR27_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP02142.1"
                     /translation="MEPNHGTVHANDLEFAFLELGSGPLALCLHGFPDTPHGWRHQLP
                     VLAEAGYRAVAPWMRGYAPTGIPADGCYQQGALAADANALHEALGGDGDAVLIGHDWG
                     ATAVYPAVNHEPDRWKAMVAAAVPHPVAMAGMIFDYDQLKRSFYMFVFQHPLAEAIVQ
                     QDDLSFIDRLWADWSPGLDEGVAATAVQHVKDALRDPDHLTAALGYYRATLGGVGLDA
                     RYDEIQAAGAQPVEIPALYLHGADDGCIGPDFAEAGAGFLTDERSRTAVIEGAGHFLQ
                     LERPERVNAEILDFLADVAR"
     gene            6781..>7275
                     /locus_tag="VLR27_01490"
     CDS             6781..>7275
                     /locus_tag="VLR27_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSP02143.1"
                     /translation="MTARPPLPDGFVAVVKQDCPTCQLVEPVLVQLVDAAELVAVVSQ
                     DDPSFPDLSTASSAPERTFDEDLAISYLWDIETVPTLLRIEDGEEVERIVGWDRQQWQ
                     DLTGLPDLGPDLPDWRPGCGSLSVDPMRADELAGRFSASGLRSRRVEFADLEDDFEAM
                     WDRGW"
BASE COUNT         1104 a         2495 c         2680 g          996 t
ORIGIN      
        1 gccgggcctg ggggcagagg ctgtcgcact ccgccgtggt cacgagcgtg ggcggcagca
       61 ccgacaggtc gcggcgggcc gggatgagct cgggatcggc cccggccggg tcgtcgccca
      121 ggtagccctc gaacagcttc gtggtcccct cgtggtcgag ccagagcacc ggcgggcagt
      181 cgggatggcg gccgtcgtag ggcccgccgt cgggatcggt gaccgggtac gccagccaca
      241 aggcggccgg cgcagctgcc tcgccggcga gggcgagggc cgtgatcgct gccaggtgcc
      301 cgccggccga cgccccgccg agcgcgaccc ggtggggatc gacgccgcgc tcggcaccgt
      361 ggtcgaccac ccaccgctgg acggcgagca cgtcctcgag cgctgccggg tgccggtggg
      421 cgggcgccag ccggtagtcc accgtcacca ccacgcccgg caggccggcc gcgacccgct
      481 gggcgaccga gtcggcctcg ggcatgtcga ggtcgccgaa ctgccagccg ccgccgtggg
      541 cccagaccag cgtcggggcg agctcgtcgg tggccagcgc ggtcgaccgg taccagcgca
      601 cggggatcgg gccgcccggg ccgggcacca cgccgtccgt gcggaccacg ccgtcgacga
      661 ccatcggccc cgctggcggg accccctcca cctcacccga cagcagctgg agcacgtggg
      721 cgtcccacag gtgggcgacc cgcgccgaga tcgctgggtc gagtgccatc gcccaacact
      781 gccaccgttc tcggtgcatg gcgttgcgtc agcgcaacgt ggtgcaccga gaacggtgct
      841 gaccgacggt cagaccggtt ggccgccggc ccaggcggcg aacagcgcgg cgtagtggcc
      901 gtcggcggcg atgagctcgt cgtgggtgcc gacctcggcg agggagccgg cggcgacgac
      961 ggccacgcgg tcggcccggg cggcggtggt caggcggtgg gcgatcacga tggtggtgcg
     1021 gccctcggag aggcgctcca tggcctcctc gacgatggcc tcggtgcccg ggtcgaggga
     1081 ggacgtggcc tcgtcgagca ccaggatctc ggggtcggcc agggcggcgc gggccaggga
     1141 gatcagctgc ttctcgccgg cggagaaccg gcttccccgc tcgcggacct cggtgtggag
     1201 gccctcgggc aggtcggcga agcggtcgag gatgccgatg gagtccatgg cggcgacgat
     1261 gtcgtcgtcg gtggcgtcgg gccgggccag gcggacgttc tccatgaggg tgccgttgaa
     1321 gaggaacccc tcctggggga cgacgaccat cgtccggcgc agcgaggcca gtgtggcgtg
     1381 gcgcaggtcg accccgccga tcgacaccga accctcggtc gggtcgtaga agcgcacgat
     1441 cagcttggcg agcgtggact tgccggcacc ggtcgggccg acgagcgcga gccgctcccc
     1501 tggttcgatc cggaggtcga ccccctcgag gaccgtggtg tccgggtccg aggcgtagtg
     1561 gaagccgaca ccggcgatct cgatcgcgcc gcgccccggc aggtcgacgg ccccgggtcg
     1621 ctctggcacg tcgaccccgg tgtcgagcag cgtgtacagc ttctgcaggc cggcgccggc
     1681 ggcctggacg gtgttgaaca gctggctgag ctgctggatg ggctcgaaca ggttcgagag
     1741 cgtgaggatg aagaacgtca cggtgccgat cgtgagcagg ccctcatgga cccagaaccc
     1801 gccgatgccc acggcgaggg cggtggtgcc caggccggcg atctcgatga ccggcaggta
     1861 ccaggccgag atcttcaccg accgcatgtg ggcgtcgtag agctggcggt tgtgggtctc
     1921 gaaccgggtg gtctgcacgt cctcccgggc gaaggcctgc acgacccgca cgccggagat
     1981 gccctcctgc agctcggaga gcgtggcgcc gatgccgtcg cggacgtcga ggtaggcctc
     2041 gttcgactcc cgctggaact tgcggctggc gatcacgacc ccggggacgg cgaccaggca
     2101 cagcgccagc atctgggggc tcacgacgcc gagcaccacg accgacacga cgagcagcag
     2161 gccgttctgc acgaacatga gcaggcccat ctgcaccagc tcggacagcg agtcgatgtc
     2221 ggaggtcatc cgggacacga ccacgccggc cttctcccgg tcgtagaagg gcatcgacag
     2281 ccgctgcagg tggtcgaaca ccctgatgcg cagctcgcgc aggaagtcct cgccgatgcg
     2341 ggcgatggcc agcacctggc gccggtagac gacgtaggcg atgaccgcca ccacgacgta
     2401 cgcgatgacg gctcggttga cgacgtcccc gtccttgggc ttgatgccct cgtcgatgcc
     2461 gtaccggacg aggaacgggc cggccagcgt ggacgccgtg tagaggacgg cgaacagggc
     2521 ggcggcgagg agccggcgcc ggtaggggcg caggaagccg taggtccgct tgaggacgtg
     2581 gaccgcggtg tcgcggtcga gctgctcgtc ctggtcgacg ccacctccca tccaccccat
     2641 caggcccgca cctccggcga gtcgggatcg acgtcggtcg cttcgagctc ctcgagggcc
     2701 gcggcggcca gcacctcgcg gtagcggggg ttggtggcca gcagctcgtc gtgggtgccg
     2761 gtggccacga cccggccctc gtcgaggagc acgacccggt cggcgagcgc gatcgtcgcc
     2821 gagcggtggg cgatgacgat ggtggtgcga ccctccatca cctcgccgag ggcatcgcgg
     2881 atctcgtgct ccttggtggg gtcgaccgag ctggtggcat cgtcgaggat cagcacccgg
     2941 gggtcggcga ggatggcccg ggcgatggcg atgcgctggc gctgtccgcc ggacagggag
     3001 tagccccgct cgccgatcac cgagtcgtag ccgtgctcga gggactcgat gaactcgtcg
     3061 gcgccggcga gccgggcggc cgtgcggatc tggtccatgg acgcatcggg ttccgcgaag
     3121 gcgatgttgg ctgcgatgga gtcggagaag aggaacgtct cctcgaagac gatgccgatg
     3181 gcccgccgca gctcggcgac ccgcacgtcg cggacgtcga cgccatcgag cagcacccgt
     3241 ccgctgtcga cctcgtagaa gcgcgggatc agacggccca ccgtggactt gccgcagccc
     3301 gtcgccccga cgagggcgac cgacgtgccg gcctcgacaa ccaggtcgaa gtcgtgcagc
     3361 accgggacgc tgccgtcgcc gtagctgaac tcgacgtgct cgaagcgcac ctcaccccga
     3421 ccgggcggca gcccgctggc gtcgggacgg tcgacgatga cggggtcggt ggagagcacc
     3481 tcggcgaccc gctgggcggc ggcggaggcc cgctggccgt tggcgatgat catcccgagc
     3541 atgcgcagcg gccagatgag gaggacgacg tagaagttga acaggacgaa ggagccgagg
     3601 ctgatctggt cgttgatgac ccggtgcccg ccgacgccga ggaccgtgat gagcccgatg
     3661 ttcggcagca gctcgagcgc gggcaggaag accgagcgga tcttcgaggc gttcatcgac
     3721 tcgcggtaga cgtcgtcggc agtgcgacgg agacggccgc ggacgacgtc ctcggcgccg
     3781 aagcccttga cgacgcggac gccggcgacg gactcctcca ccacggaggc gagctcggcg
     3841 gactcgcgct ggatgcccat gaccgatggg aacagccggc tgccgaagac cttggccacc
     3901 acgttgatga acggcagggc ggccagcgac agcacggcga gcagcgggtc gatgatcagc
     3961 aggatcacgg tgacgccgag caccgtcatg ccgttggcga tggtgagcgg gatcatcacc
     4021 acgaagttct ggaggtgctg caggtcgatg ttgccgcggc tcatcagctg cccggcctgc
     4081 acctcgtcgt ggtaggcgaa gtgcagccgc tggacatggg cgaacagccg gtcgcggagg
     4141 cgcttctcca ccagccgggc ctcacggaag gccaggtacc gccgggagcc ggtgagcacc
     4201 gccgacagca ggccgaccac gaagatggcc acggcccaga tggcgaggga cgacatgtcg
     4261 tcgttctcga tgccgttgtc gatggcgcgc tgcagcagca cgccgacgcc gaccttgctg
     4321 aacgaccaga agagcccgac cacgacaccg gcgatgaccc cagctcgtcg tggacgcagc
     4381 gtgtcggcga tgagtcgcca gccggcgcgt gtgtccgtcc cctcgatccg gctgacggta
     4441 accgacgaat tgacccgccg gtcaactact tcaggttggc gtagccaccc cggtagaaga
     4501 tcagcggcga gccctcgttc gcgacgtcga ggccgagcac ccgaccgacc acgatctcgt
     4561 ggtcgccacc gtcgtggacg tcctcgatcg cgcagtcgat ccaggccagg acgccgtcga
     4621 tcagcggcga gccggactcg gccggcttcc agccgatgcc gtcgaacttg tcgtcgccgc
     4681 tggtggcgaa gacccggcac acgtcctcct ggtcggcccc gaggacgttg accgagaagc
     4741 tgcccgagtc ggccatgcgg gcccacgacc cagacgagcg atcgacgcag aagccgacga
     4801 gcgccgggtc gagcgagagg gagaagaacg accccacggc gagcccgacc ggctcgtcac
     4861 cgtccatgcc ggcgatcacg gtgacgccgg tggggaagtg cccgagcacc tgtcggtagg
     4921 tggcctgatc gatcgcgctc atgggcggcg acgctagagg acgatggtga gtccctcgga
     4981 agcggccagg acctcttcga cccgtggacc agcgatcgct cgggcctgct cgacgaggca
     5041 gtcgaggaac acgtcgtcgt gggtggggtc gtggtggaac agcgccaggc ggcggacgcc
     5101 ggcctcggcc gcgacgtgca cggcgaactc caccgtgcag tgaccccagt cgcgcttctt
     5161 ggcgaactcc tcggacgtgt actgcgagtc gtggatcagc aggtcggcgc cggcgcagag
     5221 ctcgaggacg tcgtcggtca cgacgaggct ctcgtcgagg ggcatctggt ggtcggagat
     5281 gtagacgacg gtggcgccgc cgagctccac ccggtacccg ttggtgacgc tggtgtgcgg
     5341 cacatcgcgc accttcacct tggcgtcgcc gacggcgagc tcggtgtcgc gcaccgtgtg
     5401 gaacgtgatg tcgcccggaa ggtcaccgag gcggacgggg aagaggggcg ggcagacgaa
     5461 ctggtcgaag cagtcgtcca gggtgcgctt gccgtccagc tccggcggcg ggccgtagac
     5521 gtcgagccgg gcaccgggct gcagcaccgg tgcgaagaag ggcaggccct ggatgtggtc
     5581 ccagtgcacg tgcgtgacga gcgcgtgccc gcggaagctg ccgtcgtggg gcagcaggtc
     5641 gccccacgtg cgcagtccgg tgcccaggtc gaggacgatc gggtcgtgcc cgtgctcctc
     5701 gatcaccgcg cacgacgtgt tcccgccgta gcggtggttg tcgtcgtgcg ggcacggggt
     5761 cgacccccgg accccgaaga acgtgacctg cacggttctg gccccctgtc tcgctggagg
     5821 tggcagggta ctcatcgctc gtaccgccgc cacagcggtg ttgcaccggt cacatcccca
     5881 ctcaggagcc tgcatggaac cgaaccacgg caccgtccac gccaacgacc tcgaattcgc
     5941 cttcctcgag ctcggttccg ggccgctggc gctgtgcctg cacgggttcc ctgacacgcc
     6001 gcacgggtgg cgccaccagc tgcccgtgct cgccgaggcc gggtaccggg cggtcgcgcc
     6061 gtggatgcgc ggctacgcgc cgacgggcat cccggcggac ggctgctacc agcagggagc
     6121 actggccgcc gacgccaacg cgctccacga ggccctcggc ggcgacggcg atgcggtgct
     6181 catcgggcac gactggggtg cgacggcggt gtacccggcg gtcaaccacg agccggaccg
     6241 ctggaaggcg atggtcgccg cggcggtccc ccacccggtg gccatggccg ggatgatctt
     6301 cgactacgac cagctcaagc ggtcgttcta catgttcgtg ttccagcacc cgctggccga
     6361 ggcgatcgtg cagcaggacg acctctcctt catcgaccgc ctctgggccg actggtcccc
     6421 ggggctcgac gagggcgtgg cggcgaccgc ggtgcagcac gtcaaggacg cgctgcgcga
     6481 ccccgaccac ctcaccgccg cgctcggcta ctaccgggcc acgctcggcg gcgtcggtct
     6541 cgacgcccgc tacgacgaga tccaggcggc cggtgcccag ccggtcgaga tcccggcgct
     6601 gtacctgcac ggcgccgacg acggctgcat cggtccggac ttcgccgaag ccggcgccgg
     6661 cttcctcacc gacgagcgca gccgaaccgc cgtcatcgag ggggccgggc acttcctgca
     6721 gctcgagcgg cccgagcggg tgaacgccga gatcctcgac ttcctcgccg acgtggcccg
     6781 atgaccgccc gcccgccgct ccccgatggc ttcgtcgccg tcgtcaagca ggactgcccc
     6841 acctgccagc tggtcgagcc ggttctcgtg cagctcgtcg acgccgccga gctcgtggcg
     6901 gtcgtgtccc aggacgaccc gtcgttcccc gacctgtcga cggcgagctc ggcaccggag
     6961 cgcaccttcg acgaggacct cgccatctcg tacctgtggg acatcgagac cgtgcccacg
     7021 ctgctgcgga tcgaggacgg cgaggaggtc gagcgcatcg tcggctggga ccgccagcag
     7081 tggcaggacc tcaccggcct gcccgacctc ggtcccgacc tgcccgactg gcgtccgggt
     7141 tgcggctcgc tctcggtcga tccgatgcgc gccgacgagc tggccggtcg gttctcggcc
     7201 agcggcctgc gcagccggcg cgtcgagttc gccgacctcg aggacgactt cgaggccatg
     7261 tgggaccggg gctgg
//
LOCUS       DATISU010000029         6052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_606647, whole genome shotgun sequence.
ACCESSION   DATISU010000029 DATISU010000000
VERSION     DATISU010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606647"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..451
                     /locus_tag="VLR27_01495"
     CDS             53..451
                     /locus_tag="VLR27_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02144.1"
                     /translation="MGRAGTLTAAVGIVLAGLPGANPAGASTTDVATSRAVVVTVAIS
                     VQDLAPAACARMDLTTLVTDADRTGAKIQGSNGNDLIIGTPGSDRLDGRNGDDCLIGG
                     AGDDQLDGGNGFDVCLPGDDPADSTSRCEA"
     gene            complement(370..1188)
                     /locus_tag="VLR27_01500"
     CDS             complement(370..1188)
                     /locus_tag="VLR27_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP02145.1"
                     /translation="MVRALIVHGDAVERQRVADALGAPGSGVDVDVVADGLARLGGAV
                     EGVYDVAVVAVRLPSQSGISVLHQIRRDHPGLAVVMLGGPDAGDAELAVIALEADADD
                     HCSPDVSDRELVLRVQRAVGRRALLVAAAGGSGGGDGAVERDGLALDPVTRSASVDGV
                     QLDLTVKEFDLLRTFASSPGRVFSRSDLLELVWRTNAADHSVDTVTEHVYRLRGKLAG
                     TGSPHGWIETVRGAGYRFAVPAAETEPDQASHLLVESAGSSPGRHTSKPLPPSS"
     gene            1273..2373
                     /locus_tag="VLR27_01505"
     CDS             1273..2373
                     /locus_tag="VLR27_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSP02146.1"
                     /translation="MEADALAGLDDLAILSTIVDRLTDVVFVLGDDDLMHWRRRSSRL
                     RVHLPDNAQVLGIPMLERVHPADLPSVLDTLEQMRLGHAPEATMRVRIFDANDPSIIH
                     DEDLRAFDARDVPGVGGIVVAVEIRDTRGAFPREVHGDDFSIADVAPIGLAVLATGDR
                     IVYANALFRAHLGLEGRTAITVTSIQGLHEAIASARAGGLSSAVVSHRSRTLRVVGRR
                     IADNGTTSIALSTTDITAEHEAMAALARSQETWRATFEHAPAGIALVDPTGRFLEINR
                     AWTAITGYEPAELVGRSFADITHPDDLEPDLALVRELLAGNQPSYRVLKRYLHRDGHT
                     VPVDLWCSIVRDGAGEPVHFVAQILEQSDAAR"
     gene            complement(2380..5115)
                     /gene="ppc"
                     /locus_tag="VLR27_01510"
     CDS             complement(2380..5115)
                     /gene="ppc"
                     /locus_tag="VLR27_01510"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HSP02147.1"
                     /translation="MHESTPDQAMRADIRRLGHQLGQAIARQEGDEMLDLVERVRVLA
                     RRIRTGDHAGMASDRDLAAVLRGVDPVDAIRLVRAFTLYFHLANATEQVHRIEELNQR
                     TADAGRITDTVDRLMGAGHDAGAIADAVARTELRPVFTAHPTEASRRSILDKLTEVAT
                     RLERRQHPAATATEQRRIDRRIDELIDAMWQTDELRRERPTPSDEARSVLYVLEQVAR
                     QGLPELLDDLALALERVGGSLPPDAAPIRFGSWVGGDRDGNPNVTAETTAEVLALHRE
                     RALGLLREELDHLRGELSTSDRIRAASDELRAAVEADRIPYADVHDRIARRVEGEWYR
                     LRLAVIAERLRCTAQSSESSEPRAYRRPAELDADLAVLDRSLREHGGALLADGPLART
                     RRLLATVGFHLATLDLREHAARLHESLGGLFQGCGIEYPSDRAARETLLVSELESRRP
                     LAPPGTPLPDDDPLHAFRALRDVLDQRGDDVVESYIVSMTKGVDDILAAVLLAREVGL
                     VDLSAGVARLGFVPLFETIDDLRSIEDVLERLLAVPGYRRIVELRGAQEVMVGYSDSN
                     KDGGITTSQWEIHKALRRIRDLAERHDLPIRVFHGRGGTIGRGGGPTNTSILAQPAGL
                     IQGEVKITEQGEVIADKYGRPDLAQRNLDLAMSAVIEATVARRGPRHDDATRARWVEV
                     MDLASDAAYAAYRRFVDDPDLPEYFRTSTPVEELGQMNIGSRPARRTTQGDGGLTLAG
                     LRAIPWVFGWTQSRQIIPGWFGVGTGIEAALAAGHGDTLAEMLREWTFFRTFLSNVEM
                     TLAKTDLGIAARYVEALVPPGLEHLFDVVRDEHARTLAAVTALTGRELLGDLPTIART
                     LRVRDRYLDPIHVLQVALLERTRALGDGSSSEARRLRRALLLSVNGVAAGMRNTG"
     gene            complement(5138..>6052)
                     /locus_tag="VLR27_01515"
     CDS             complement(5138..>6052)
                     /locus_tag="VLR27_01515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSP02148.1"
                     /translation="AAEGLFRAVFDGSPVATAVVDLAGRFVQVNDALAMICGRTADDL
                     VGRTFAEVTHPDDLAVDEELLAEVRSGRRGGYQMEKRFVHRAGHEVWVELTVSTVRDA
                     AGAITHFVSHVEDISGRKALIDTADSSEELAYWATHDHLTGLPNRRYLDSYLTSSLGP
                     RRRADDRLVVLFLDLDDFKPVNDRHGHQTGDEVLRAIARRLRNTAREDELVARYGGDE
                     FVVVTTRARTAYDVSLLAERILSAVRTPIVGLTAEPIVVGASVGIGHANRGEDPTDLL
                     RRADAASYRAKRAGKGRIELAPSTSDGS"
BASE COUNT          853 a         2157 c         2222 g          820 t
ORIGIN      
        1 ccctcgtgct cggcctgctc ggtcgggtgc cggtgcccga ggagggcgac gcgtgggtcg
       61 agccggcact ctgacggcgg cggtcgggat cgtgctcgct gggctcccgg gcgcgaaccc
      121 agccggcgcg tcgaccacgg acgtcgccac cagtcgggcc gtcgtcgtca ccgtcgccat
      181 ctcggtccag gacctcgccc ctgccgcgtg cgcacggatg gacctcacca ccctggtgac
      241 cgacgccgac cgcacgggcg ccaagatcca gggctccaac ggcaacgacc tcatcatcgg
      301 cacccccggc agcgaccgcc tcgacggacg caacggcgac gactgcctca tcggcggcgc
      361 cggcgacgat cagctcgacg gtggcaacgg cttcgacgtg tgcctaccgg gcgacgaccc
      421 cgccgactcc acgagcaggt gcgaggcctg atcgggctcg gtctcggcgg ccgggacggc
      481 gaagcggtag ccggcgccgc gcacggtctc gatccagccg tgcggggagc cggtgccggc
      541 cagcttcccg cgcagccggt acacgtgctc ggtgacggtg tcgacgctgt gatcggcggc
      601 gttcgtccgc cacaccagct cgagcaggtc ggaccgggag aagacgcgac ccggcgagga
      661 cgcgaaggtc cgcagcagat cgaactcctt caccgtcagg tccagctgca cgccatcgac
      721 cgatgccgaa cgcgtcacgg ggtccagggc cagaccgtcc cgctcgacgg caccgtcacc
      781 acccccgctc ccacccgccg cggcgacgag cagggcgcgc cgcccgacgg cacgctgcac
      841 gcgcagcacg agctcgcggt cggacacatc gggcgagcag tggtcgtcgg cgtcggcctc
      901 gagggcgatc acggccagct cggcatcccc cgcgtccgga ccgccgagca tcaccacggc
      961 gaggccgggg tggtcgcgcc ggatctggtg gagcaccgag atgccgctct gcgacgggag
     1021 ccgcacggcc accaccgcca cgtcgtagac gccctcgaca gctccgccga gtcgagcgag
     1081 gccgtcggcg acgacgtcca cgtccacgcc cgagcccggg gcgccgagtg cgtccgccac
     1141 gcgctggcgt tcgacggcat ccccatgcac gatcagcgct cggaccacgc tccagtatga
     1201 ccacgccgag gcgctgcgtg gtcacactgc ggatggcacc agcaacggag gcggcaccgg
     1261 taccgtccgc acgtggaagc agacgctctg gcgggcctgg acgatctcgc gatcctgagc
     1321 acgatcgtcg atcggctcac cgatgtcgtc ttcgtgctcg gcgacgacga cctcatgcac
     1381 tggcgccggc gcagctcgcg cctgcgggtc cacctgcccg acaacgccca ggtcctcggc
     1441 atcccgatgt tggagcgcgt ccacccagcg gatctcccct cggtgctcga cacgctcgag
     1501 cagatgcggc tcgggcacgc gcccgaagcg acgatgcggg tgcgcatctt cgacgcgaac
     1561 gacccgagca tcatccacga cgaggacctg cgcgccttcg atgcccgcga cgtcccgggc
     1621 gtcggcggca tcgtggttgc agtggagatc cgggacacgc ggggagcctt cccgcgtgag
     1681 gtccacgggg acgacttctc catcgccgac gtcgcaccga tcggtctggc cgtgctcgcc
     1741 accggcgacc gcatcgtcta cgccaacgca ctgttccgcg cccacctcgg cctcgagggt
     1801 cgcaccgcca tcaccgtcac ctcgatccag gggctccacg aggcgatcgc cagcgcccga
     1861 gcgggtggcc tgtcctccgc cgtcgtgtcg caccggtctc gcacgctgcg ggtcgtcggt
     1921 cgccgcatcg cggacaacgg caccacgagc atcgccctgt cgaccaccga catcaccgcg
     1981 gagcacgagg ccatggccgc cctggcccgt tcgcaggaga cgtggcgagc gaccttcgag
     2041 cacgcgccgg cgggcatcgc cctggtcgat ccgaccggtc gcttcctcga gatcaaccgg
     2101 gcgtggacgg ccatcaccgg ctacgaaccg gcggagctgg tcggccggtc cttcgccgac
     2161 atcacccacc ccgacgacct cgaacccgac ctcgccttgg tccgcgagct gctcgccggc
     2221 aaccagccca gctaccgcgt cctcaagcgc tacctccacc gcgacggcca caccgtgccg
     2281 gtcgatctgt ggtgcagcat cgtgcgcgac ggcgccggcg agcccgtgca cttcgtcgcc
     2341 cagatcctcg agcagagcga cgcagcacgc tgaacctgct cagccggtgt tgcgcatgcc
     2401 ggcggcgacg ccgttgacgc tgagcagcag cgcacggcgc agccggcgcg cttccgagct
     2461 gctcccgtcg cccagtgcac gggtgcgctc gaggagggcg acctggagga cgtggatcgg
     2521 gtcgaggtag cggtcgcgca cgcgcagggt gcgggcgatg gtgggcaggt cgccgagcag
     2581 ctcacggccc gtcagcgcgg tgaccgccgc gagggtccgg gcgtgctcgt cccgcacgac
     2641 gtcgaagagg tgctccagcc cgggcggcac cagggcctcg acgtagcggg ccgcgatgcc
     2701 gaggtcggtc ttggccagcg tcatctccac gttggacagg aacgtgcgga agaacgtcca
     2761 ctcccgcagc atctcggcga gcgtgtcccc gtggccggcc gccagcgccg cctcgatccc
     2821 ggtgcccaca ccgaaccagc ccgggatgat ctggcgggac tgggtccagc cgaacaccca
     2881 cgggatggcg cgcaggccgg cgagggtcag gccgccgtcg ccctgggtcg tgcgccgggc
     2941 cggacgggag ccgatgttca tctggcccag ctcctcgacc ggtgtggagg tgcggaagta
     3001 ctcgggcagg tcggggtcgt ccacgaagcg gcggtaggcg gcgtaggccg catcggaggc
     3061 aaggtccatg acctcgaccc agcgggcgcg ggtggcgtcg tcgtggcgcg gcccgcggcg
     3121 ggcgaccgtc gcctcgatca ctgcggacat ggccaggtcg aggttgcgct gggcgaggtc
     3181 ggggcggccg tacttgtcgg cgatgacctc gccctgctcg gtgatcttga cctcaccctg
     3241 gatgagtccg gcgggctggg ccaggatcga ggtgttcgtc ggaccccctc ctctgccgat
     3301 ggtgccgccc cgaccgtgga acacgcggat gggaaggtcg tggcgctcgg cgagatcccg
     3361 gatgcggcgc agcgccttgt ggatctccca ctggctggtg gtgatgccgc cgtccttgtt
     3421 ggagtccgag tagccgacca tcacctcctg ggcgccccgc agctcgacga tgcggcggta
     3481 tccgggaacg gccagcagcc gttcgagcac gtcctcgatg ctccgcaggt cgtcgatggt
     3541 ctcgaagagc ggcacgaacc cgaggcgggc cacgccggcc gacaggtcga ccaggcccac
     3601 ctcgcgggcg agcaggaccg cagcgaggat gtcgtccacg cccttggtca tggagacgat
     3661 gtaggactcc acgacgtcgt cgccccgctg gtcgagcacg tcgcgcagcg cacggaacgc
     3721 gtgcaaggga tcgtcgtcgg gcagcggcgt gcccggcggg gcgagaggcc gacggctctc
     3781 cagctcagag acgagcaggg tctcccgagc ggcacggtcg ctcgggtact cgatcccaca
     3841 tccctggaac agcccgccga gcgactcgtg gaggcgggcc gcgtgctccc gaaggtcgag
     3901 cgtcgcgagg tggaagccga cggtggcgag cagccgtcgc gtgcgggcga gggggccgtc
     3961 ggcgaggagc gcaccgccgt gctcccggag tgagcggtcg agcacggcga ggtcggcgtc
     4021 gagctcggcc gggcgccggt aggcccgagg ctccgacgac tccgacgact gggcggtgca
     4081 gcggagccgt tcggcgatga cggcgaggcg gagccggtac cactccccct ccacccgccg
     4141 ggcgatccgg tcgtgcacgt ccgcgtaggg gatgcgatcg gcctcgacgg cagcgcggag
     4201 ctcgtcgctg gcggcccgga tgcgatcgga ggtgctgagc tcgccgcgca ggtggtcgag
     4261 ctcttcgcgg agcaggccga gggcccgctc gcggtgcagc gcgaggacct cggcggtcgt
     4321 ctccgcggtc acgttggggt tgccgtcccg gtcgccaccg acccagctgc cgaagcggat
     4381 cggtgcagca tccggcggca gggagccacc cactcgctcg agggcgagcg ccaggtcgtc
     4441 gagcagctcg gggagtccct gccgggccac ctgctcgagc acgtacagca ccgaccgggc
     4501 ctcgtccgag ggcgtggggc gctcgcgccg gagctcgtcg gtctgccaca tggcgtcgat
     4561 gagctcgtcg atgcggcggt cgatgcgacg ctgctcggtg gcggtggcgg ccgggtgctg
     4621 gcgacgttcg aggcgggtgg cgacctcggt gagcttgtcg aggatggacc ggcgcgacgc
     4681 ctcggtggga tgggcggtga acaccggtcg cagctccgtc cgggcgaccg catcggcgat
     4741 ggcgccggcg tcgtggccgg cccccatcag gcggtcgacg gtgtcggtga tccgtccagc
     4801 atccgcggtg cgctggttca gctcctcgat gcggtggacc tgctcggtgg cgttggccag
     4861 gtggaagtag agggtgaacg cccggaccag gcggatcgcg tcgacgggat ccacccctcg
     4921 cagcacggcg gcgaggtccc ggtcgctggc catcccggcg tggtcgccgg tgcggatgcg
     4981 acgggcgagc acgcgcaccc gttcgaccag gtcgagcatc tcgtcgccct cctggcgggc
     5041 gatggcctgg ccgagctggt ggccgaggcg tcggatgtcc gctcgcatgg cctggtccgg
     5101 cgtggactcg tgcatgcggg cgagggtaac ggcgcggtca ggaaccgtcg gacgtggatg
     5161 gcgcgagctc gatgcgcccc ttgccggccc gcttggcccg gtacgaggcg gcgtcggccc
     5221 gccggagcag gtcggtcggg tcctccccac ggttcgcatg gccgatgccc acgctcgccc
     5281 cgaccacgat cggctccgcg gtcagaccga cgatcggggt ccgcacggcg ctcaggatcc
     5341 gctcggcgag cagcgacacg tcgtaggccg tgcgcgcccg ggtcgtgacg acgacgaact
     5401 cgtctccgcc gtagcgagcg acgagctcgt cctcccgagc cgtgttgcgg aggcggcggg
     5461 cgatggcccg gagcacctcg tcaccggtct ggtgtccgtg ccggtcgttg accggcttga
     5521 agtcgtccag gtcgaggaag agcacgacga ggcggtcgtc ggcgcgacgg cggggcccga
     5581 ggctcgaggt caggtagctg tcgaggtagc ggcggttggg caggccggtg aggtggtcgt
     5641 gcgtggccca gtacgccagc tcctccgacg aatccgccgt gtcgatcagc gccttgcgcc
     5701 cggagatgtc ctcgacgtgg gagacgaagt gcgtgatggc acccgcggcg tcgcgcacgg
     5761 tcgagaccgt cagctcgacc cacacctcgt ggccggcccg gtgcacgaac cgcttctcca
     5821 tctggtagcc gccgcggcgc ccggaccgga cctcggccag cagctcctcg tcgacggcca
     5881 ggtcatcggg gtgggtgacc tcggcgaagg tccgaccgac gagatcgtcg gcggtgcgac
     5941 cgcagatcat ggccagggcg tcgttcacct gcacgaagcg accggcgaga tcgaccacgg
     6001 cggtggcgac gggggaaccg tcgaagaccg cccggaagag gccctcggcc gc
//
LOCUS       DATISU010000030         1513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_793024, whole genome shotgun sequence.
ACCESSION   DATISU010000030 DATISU010000000
VERSION     DATISU010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793024"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..1490
                     /locus_tag="VLR27_01520"
     CDS             21..1490
                     /locus_tag="VLR27_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HSP02149.1"
                     /translation="MSLPRVIAVAPGSPAARAGLVEGDEILSLDGVQPRDVIEWRLLV
                     DEPEVVLDVRRGGLERQVTVAKGAGEPLGADVDAAIFDEVQTCDNHCEFCFIYQLPPG
                     MRRSLYLKDDDYRLSFLYGNFTTLTRFTEIDLERVVTEGLSPLYVSIHATDPEVRADL
                     LRNRRGATSLRWLRALLDHDVTVHGQIVVCPGVNDGAVLADTLAGVLDEYPELASLAV
                     VPLGISDHSDESRMRPHTLAEAVTVVDIVEDWQDVYRTVLGHRLVYAADEYYLLARRP
                     FPPAEAYEGFDMHEDGIGMARTFEAEFHGAADEVTRPQAGFFAWVDGAPAEGYRAPRT
                     TSTACGTSAPIGPDVPSGGTNEPIGPGVPVQLGRRRPAPDAPIGVLTAPYGAQVLAPL
                     LAGVDRDDVRIVEVPNRFFGGNIGVTGLMVGEDVARVLADQPAGHRYLLPDVCLSQDR
                     FLDGTTIADLPRPVEVVPTDGAALRAALGLPLPASPTPA"
BASE COUNT          207 a          601 c          492 g          213 t
ORIGIN      
        1 ccggcggtac cctgtcgccg gtgagcctgc cccgcgtcat cgcggtcgcg cccggatcac
       61 ccgcagcccg agcgggtctc gtcgaaggcg acgagatcct ctccctcgac ggggtccagc
      121 cgcgcgacgt catcgagtgg cgcctcctgg tcgacgagcc ggaggtggtc ctcgacgtgc
      181 gccgaggcgg cctcgagcgc caggtcaccg tggcgaaggg tgccggcgag ccgctcggtg
      241 ccgacgtcga cgcggcgatc ttcgacgagg tgcagacctg tgacaaccac tgcgagttct
      301 gcttcatcta ccaattgccc ccggggatgc gccggagcct gtacctgaag gacgacgact
      361 accgcctgtc cttcctctac gggaacttca ccaccctcac ccggttcacc gagatcgacc
      421 tcgagcgggt ggtcaccgag gggctcagcc cgctgtacgt gagcatccac gccaccgacc
      481 ccgaggtccg tgccgacctg ctccggaacc gacggggtgc caccagcctg cgctggctgc
      541 gcgccctgct cgaccacgac gtgaccgtgc acggtcagat cgtggtgtgc ccgggcgtga
      601 acgacggggc ggtcctggcc gacaccctcg ccggcgtgct cgacgagtac cccgagctgg
      661 ccagcctggc ggtggtgccc ctcggcatca gcgaccactc cgacgagtcc cggatgcggc
      721 cccacaccct cgccgaggcc gtgacggtcg tcgacatcgt cgaggactgg caggacgtct
      781 accggaccgt gctcggccac cgcctggtct acgccgccga cgagtactac ctgctcgccc
      841 gacgaccgtt cccgcccgcc gaggcctacg agggcttcga catgcacgag gacgggatcg
      901 gcatggcccg cacgttcgag gccgagttcc acggcgcggc cgacgaggtc acccgacccc
      961 aggccggctt cttcgcctgg gtcgacggtg cgccggctga gggctaccgc gccccccgca
     1021 cgacgagcac ggcctgcggg acgagcgcac cgatcggtcc cgacgttcca tccggtggaa
     1081 cgaacgaacc gatcggtccc ggcgtcccgg tgcagctcgg gcgacggcgt cccgcccccg
     1141 acgcgcccat cggcgtgctc accgccccct acggcgccca ggtgctcgcc ccattgctcg
     1201 ccggcgtcga ccgcgacgac gtgcgcatcg tcgaggtccc gaaccgcttc ttcggcggca
     1261 acatcggcgt caccggcctc atggtcggcg aggacgtggc ccgcgtgctg gccgaccagc
     1321 ccgccgggca ccgctacctg ctgcccgacg tgtgcctgtc ccaggaccgc ttcctggacg
     1381 gcaccaccat cgccgacctg ccccggccgg tcgaggtcgt acccaccgac ggggccgcgc
     1441 tgcgcgccgc cctcggcctc cccctcccgg cgtccccgac gcccgcctga atcgagagct
     1501 ccatgtcctc ccc
//
LOCUS       DATISU010000031         5894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_187742, whole genome shotgun sequence.
ACCESSION   DATISU010000031 DATISU010000000
VERSION     DATISU010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187742"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VLR27_01525"
     CDS             <1..922
                     /locus_tag="VLR27_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HSP02150.1"
                     /translation="VLSHEADDFVAAHDLVKGAMTLIEPDRATELYEAMGPGIEVSGG
                     SAANTIAGVASFGGRAAYLGKVHDDQLGVVFAHDMRANGVVFRSPPASDGPPTGRCLI
                     IVTPDAERTLNTYLGASAYFSPDDVDAELIELSRYTFLEGYLFDRPEAKAAYRAASEI
                     AHGAGRKVALTLSDTFCVERHIDEWRPLVADQVDVLFANDAELRALYGIHLDSAVDRA
                     RDEVETTCVTLGAEGSLIVTSEAIVEVPPHPVEVVDTTGAGDLYAAGVLHGMANGMPL
                     AECGRLGSIAASAVIGHTGARPGQSLAQLA"
     gene            919..1752
                     /locus_tag="VLR27_01530"
     CDS             919..1752
                     /locus_tag="VLR27_01530"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HSP02151.1"
                     /translation="MSSLRTPPDAYARAADAAAALAQRTEVERHDVVVVLGSGWRTAA
                     EQLGTPSTAFPAAELPGFHAPTVEGHGGELRSIDVDGRRVLVALGRVHAYEGHDLADV
                     VHGVRTAVLAGCSTVVLTNAAGGLGEGMSIGQPVLIADHLNLTGRSPLTGPTPDAAIG
                     PRFPDLTDLYSSRLRAMARELEPSLVEGVYAGMNGPQYETPAEIRMLQTLGADLVGMS
                     TVHEAVAAHHMGAEVLAMSLVTNLAAGLGDSLDHEDVLSVAATSADAMGSLLARLVRR
                     L"
     gene            1758..3431
                     /locus_tag="VLR27_01535"
     CDS             1758..3431
                     /locus_tag="VLR27_01535"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HSP02152.1"
                     /translation="MDARLRAAVDAWRADDPDPATVAELDALVAADDEVGLEAAFAAP
                     LAFGTAGLRGRLGPGPGRMNRAVVRRAAAGLCRWLDDHGWDGPVVVARDARHGSADFA
                     RDTAAVVAGTGRRVVLADRPLPTPALAFAVRHLGGAAGVMVTASHNPPADNGYKVYAG
                     DGAQIVAPWDREIADHMASIERVSELTLAPLDDERITDLGEDVVAAYVDGVAGLRTSD
                     GPVDLRVAYTPMHGVGLDVLQAAFARAGFAAPVVVGSQAQPDPDFPTVAFPNPEEPGA
                     MDLLLALAEEHGADLAIANDPDADRLALAVPGPPGGQTTWRVLTGDEIGLLLADHLLR
                     HSEGADRLLVTTVVSSSALAALAAVHGVEFVETLTGFKWLARAAMDRPDLRPVLGYEE
                     ALGYSVGSLVRDKDGIGAAVVAVEAVAGLLAQRSSVAQRLAELAVDLGLHRTRQRSLR
                     FEGIDGAQRMAAAVDRIRTDPPDVLGDFDITGVEDLALGQRLPPTDGVVLRGDGMRVV
                     VRPSGTEPKLKLYVEVVRLVDAVDDVGAETEAADERIDRVLADLGSRLA"
     gene            3487..3843
                     /locus_tag="VLR27_01540"
     CDS             3487..3843
                     /locus_tag="VLR27_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSP02153.1"
                     /translation="MRVLICDDEADIRFLFRMAFEARGVEVTEAVDGDDCIAQVADVC
                     PDLIVLDLFMPNRDGLSALPELKRRCPTTRVLVVTAYAAAESFNRSRARGATACFDKL
                     EFEQRIPRLVERYGAA"
     gene            complement(3862..4293)
                     /locus_tag="VLR27_01545"
     CDS             complement(3862..4293)
                     /locus_tag="VLR27_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSP02154.1"
                     /translation="MDAITLLTNDHDEVRSLFEQFRTANEADDMPRMKELQKQIFSEL
                     ETHTRIEEDIFYPAVKDTDEEELAETVDEGVQEHHVVKVLMREIEDVSGDDTFIAKMK
                     VLMENVEHHAEEEETDMFPDLRERMSQQRLDELGAEMEAAK"
     gene            complement(4356..4979)
                     /gene="upp"
                     /locus_tag="VLR27_01550"
     CDS             complement(4356..4979)
                     /gene="upp"
                     /locus_tag="VLR27_01550"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HSP02155.1"
                     /translation="MDTLVVDHPLAAQLLTRMRDERTDRAVFRQAADELALMLVYEAT
                     RALATEPVTIRTPLTETQGVRVTRPPMVVPVLRAGLGMLGPVLRLLPGSDTGFIGVAR
                     DETTHEPVPYMNSVPDDLDGRDVLVLDPMLATGGSMVFACEQLAARNPGTVTAVCILA
                     APEGVDRLGSTGLVDRLVTASVDDHLDEDAFIVPGLGDAGDRLFGTA"
     gene            4989..5657
                     /gene="deoC"
                     /locus_tag="VLR27_01555"
     CDS             4989..5657
                     /gene="deoC"
                     /locus_tag="VLR27_01555"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HSP02156.1"
                     /translation="MASVDRTAVARMIDHTLLAPEATVERVQALCAEAKELGVGAVCV
                     SPNQVVTAATALRGSAVGLASVIGFPSGAHRAEVKAAEAAAAVADGATELDMVVPLGA
                     VVAGDWAVVEAHLRAVRDAAAPPVVLKVIVESAALDDAQLDRVCAVAVDAGADYVKTS
                     TGFHPSGGASVEAVRRMRAAVGWDIGVKASGGIRDAATALAMIEAGATRIGASSSAAI
                     LDGI"
     gene            complement(5676..>5894)
                     /locus_tag="VLR27_01560"
     CDS             complement(5676..>5894)
                     /locus_tag="VLR27_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSP02157.1"
                     /translation="FECSMMDFHFSTWGYRIEPTDTGCRVTEWSEDLRPESALEFSKQ
                     MSGVDDRQARNRETMSETLERLAASLEG"
BASE COUNT          714 a         2228 c         2107 g          845 t
ORIGIN      
        1 cgtgctcagc cacgaagccg acgacttcgt ggccgcccac gacctggtca agggggcgat
       61 gacgctcatc gagcccgacc gggccaccga gctgtacgag gccatggggc cgggcatcga
      121 ggtgtcgggg ggctcggcgg cgaacaccat cgccggcgtg gcctccttcg gcggccgcgc
      181 cgcctacctc ggcaaggtcc acgacgacca gctcggcgtg gtgttcgccc acgacatgcg
      241 ggccaacggc gtggtgttcc gctccccacc ggcctcggac ggcccgccca ccggccgctg
      301 cctgatcatc gtcacgcccg acgccgagcg gaccctcaac acctacctcg gggcatccgc
      361 gtacttcagc cccgacgacg tcgacgccga gctcatcgag ctgtcgcggt acacgttcct
      421 cgagggctac ctgttcgacc gaccggaggc caaggccgcc taccgcgccg cctccgagat
      481 cgcccacggc gccggccgca aggtcgccct caccctgtcc gacacgttct gcgtggagcg
      541 ccacatcgac gagtggcggc ccctcgtcgc cgaccaggtc gacgtcctct tcgccaacga
      601 cgccgagctg cgcgccctct acggcatcca cctcgactcg gcggtcgacc gggcgcgcga
      661 cgaggtcgag accacgtgcg tcaccctcgg cgccgagggc tccctgatcg tcacctcgga
      721 ggccatcgtc gaggtgccgc cccacccggt cgaggtggtc gacaccaccg gcgccggcga
      781 cctgtacgcc gccggcgtgc tccacgggat ggccaacggc atgccgctgg ccgagtgcgg
      841 ccggctcggt tccatcgccg cctcggcggt catcggccac accggcgccc gtcccggcca
      901 gtcgctggcc cagctggcat gagcagcctg cgcaccccgc ccgacgcgta cgcacgggcg
      961 gccgacgccg cggccgcgtt ggcccagcgc accgaggtcg agcgccacga cgtggtcgtc
     1021 gtgctcggct ccggctggcg cacggcggcg gagcagctcg ggaccccgtc gacggcgttc
     1081 cccgccgccg agctgcccgg cttccacgcc cccactgtcg agggccacgg gggcgagctg
     1141 cgctccatcg acgtcgacgg ccggcgggtg ctcgtcgcgc tgggccgggt ccacgcctac
     1201 gagggccatg acctggccga cgtggtccac ggggtgcgca ccgccgtgct cgccggctgc
     1261 agcaccgtcg tgctcaccaa cgccgcgggt ggcctcggcg agggcatgtc catcgggcag
     1321 ccggtgctca tcgccgacca cctcaacctc accggtcgct cgcccctcac cgggccgacg
     1381 cccgacgcgg ccatcggtcc ccgtttcccg gacctcaccg acctctactc gtcgcgcctc
     1441 cgggcgatgg ccagggagct cgagccgagc ctggtcgagg gcgtctacgc cggcatgaac
     1501 ggcccgcagt acgagacgcc ggccgagatc cgcatgctcc agacgctcgg tgccgacctc
     1561 gtcgggatgt ccacggtcca cgaggccgtg gccgcccacc acatgggtgc cgaggtgctg
     1621 gccatgtccc tggtcaccaa cctggccgcc ggtctcggcg actccctcga ccacgaggac
     1681 gtgctgtccg tggcggccac gtcggccgat gccatggggt ccctgctcgc ccggctggtc
     1741 cggcggctgt aggcccggtg gacgctcggc tgcgcgccgc cgtcgacgcc tggcgcgccg
     1801 acgaccccga tcccgccacg gtcgcggagc tcgacgccct cgtcgccgcc gacgacgagg
     1861 tcgggctcga ggccgccttc gctgccccgc tggcgttcgg caccgccggc ctgcggggcc
     1921 ggctcgggcc ggggccgggc cggatgaacc gcgctgtcgt gcgacgcgcc gccgccgggc
     1981 tgtgccggtg gctcgacgac cacgggtggg acggtcccgt cgtcgtggcc cgcgacgcgc
     2041 gccacggctc cgccgacttc gcccgggaca ccgcggcggt cgtcgccggc accggacggc
     2101 gggtcgtcct cgccgaccgc cccctgccca cgcccgcgct ggccttcgcc gtgcgccacc
     2161 tgggcggggc cgccggcgtg atggtcaccg ccagccacaa cccgcccgcc gacaacggct
     2221 acaaggtgta cgccggcgac ggcgcccaga tcgtcgcgcc gtgggatcgg gagatcgccg
     2281 accacatggc atcgatcgag cgggtgtccg agctgaccct cgccccgctc gacgacgagc
     2341 gcatcaccga cctcggcgag gacgtggtcg ccgcctacgt cgacggggtg gccgggctgc
     2401 ggacctccga cgggccggtc gacctgcgcg tcgcctacac gccgatgcac ggggtcggcc
     2461 tcgacgtcct gcaggccgcc ttcgcccgtg ccgggttcgc ggcgcccgtg gtggtcgggt
     2521 cccaggccca gcccgacccc gacttcccca cggtggcgtt cccgaacccc gaggagcccg
     2581 gtgccatgga cctgctcctc gccctcgccg aggagcacgg cgccgacctg gccatcgcca
     2641 acgaccccga cgccgaccgc ctcgccctgg ccgtgcccgg ccccccgggc gggcagacca
     2701 cgtggcgggt gctcaccggg gacgagatcg gcctgctgct cgccgaccac ctgctccgcc
     2761 actccgaggg cgccgaccgc ctgctcgtca ccaccgtggt gtcgtcttcg gcgctcgccg
     2821 cgctcgctgc ggtgcacggc gtcgagttcg tcgagacgct caccggcttc aagtggctgg
     2881 ctcgggccgc gatggaccgg cccgacctcc gtcccgtgct cggctacgag gaggcgctcg
     2941 ggtactcggt cgggtcgctg gtgcgggaca aggacggcat cggcgccgcg gtcgtggccg
     3001 tggaagccgt cgcggggctg ctggcgcagc ggtcgtcggt ggcccagcgc ctcgccgagc
     3061 tggccgtgga cctcggcctg caccgcaccc gccagcgctc gttgcgcttc gagggcatcg
     3121 acggcgcgca gcgcatggcg gccgccgtcg accgcatccg gaccgatcca cccgacgtgc
     3181 tcggggactt cgacatcacc ggcgtcgagg acctggcgct gggccagcgc ctgccgccga
     3241 ccgacggtgt ggtcctgcgc ggcgacggca tgcgggtggt ggtccgtccg tcgggcaccg
     3301 agcccaagct caagctctac gtcgaggtcg tccgcctggt cgacgccgtg gacgacgtcg
     3361 gggcggagac cgaggcggcc gacgagcgca tcgaccgggt cctcgccgat ctcggctccc
     3421 ggttggcctg agacgacctg gagcacggct cgtcgtccgg tgcgggtccg gtcctacggt
     3481 ggtgccgtgc gggtgctgat ctgcgacgac gaggctgaca tccggttcct cttccggatg
     3541 gcgttcgagg cgcgaggcgt cgaggtgacc gaggccgtgg acggtgacga ctgcatcgcg
     3601 caggtcgccg acgtctgccc ggacctcatc gtgctcgacc tgttcatgcc gaaccgggac
     3661 gggctgtcgg ccctgccgga gctgaagcgg cggtgcccca cgacccgggt gctcgtggtc
     3721 acggcgtacg cggctgcaga gagcttcaac cgcagccggg cccgaggggc caccgcctgc
     3781 ttcgacaagc tcgagttcga gcagcggatc ccgcggctcg tcgagcggta cggggccgcc
     3841 tgaccccgca gcagcgcggc gctacttggc ggcttccatc tcggcgccga gctcgtcgag
     3901 gcgctgctgg ctcatccgct cgcggaggtc ggggaacatg tcggtctcct cctcctcggc
     3961 gtggtgctcc acgttctcca tgagcacctt catcttggcg atgaaggtgt cgtcgccgga
     4021 gacgtcctcg atctcgcgca tgaggacctt gacgacgtgg tgctcctgga cgccctcgtc
     4081 gacggtctcg gccagctcct cctcgtcggt gtccttgacc gcggggtaga agatgtcctc
     4141 ctcgatccgc gtgtgcgtct cgagctcgga gaagatctgc ttctgcagct ccttcatgcg
     4201 gggcatgtcg tcggcctcgt tggccgtgcg gaactgctcg aagaggctgc gcacctcgtc
     4261 gtggtcgttg gtcagcagcg tgatggcatc catggggtcc tctttcgacg gtggggtggg
     4321 tgccgaccct taccccgtcg tcgtccgagt cgtgcctagg ccgtcccgaa gagccggtcg
     4381 ccggcatcgc cgagcccggg gacgatgaag gcgtcttcgt ccaggtggtc gtccaccgac
     4441 gcggtgacga ggcggtcgac gaggccggtg ctccccagcc ggtccacgcc ctcgggtgcg
     4501 gccaggatgc acaccgcggt gacggtgccc gggttgcgtg ccgccagctg ctcgcaggcg
     4561 aagaccatgg acccgcccgt ggccagcatc ggatcgagca cgaggacgtc ccgaccgtcg
     4621 aggtcgtcgg gcaccgagtt catgtacggc accggctcgt gggtcgtctc gtcgcgtgcc
     4681 acgccgatga agccggtgtc gctacccggc agcaggcgca gcaccgggcc gagcatgccc
     4741 agcccggcgc gcagcaccgg cacgaccatg gggggccggg tgacgcggac gccctgggtc
     4801 tcggtgagcg gcgtgcggat ggtcaccggc tcggtggcga gggcgcgggt ggcctcgtag
     4861 accagcatca gggccagctc gtcggcggcc tggcggaaca cggcccggtc ggtgcgctcg
     4921 tcgcgcatgc gggtcagcag ctgggcggcg agggggtggt cgacgacgag cgtgtccatg
     4981 tgggcatgat ggcatccgtg gatcgcaccg ccgtcgcccg catgatcgac cacacgctgc
     5041 tcgccccgga ggcgacggtc gagcgcgtgc aggcgctgtg cgccgaggcc aaggagctcg
     5101 gcgtcggcgc ggtctgcgtg tcgccgaacc aggtcgtcac ggccgccacc gccctccgcg
     5161 gctcggcggt cgggctggcg tcggtgatcg ggttcccgtc cggtgcgcac cgcgccgagg
     5221 tcaaggccgc cgaggcggcc gccgccgtcg ccgacggtgc caccgagctc gacatggtcg
     5281 tgcccctcgg cgccgtggtg gccggcgact gggcggtggt cgaggcccac ctccgcgccg
     5341 tgcgcgacgc cgcggccccg ccggtcgtcc tcaaggtcat cgtggagtcc gccgccctcg
     5401 acgacgccca gctcgaccgg gtgtgcgccg tggccgtcga cgccggcgcc gactacgtga
     5461 agacgtccac gggcttccac ccgtccggcg gcgcctccgt cgaggcggtc cgacgcatgc
     5521 gcgccgcggt cggctgggac atcggcgtca aggcgtccgg cggcatccgc gacgccgcga
     5581 cggcgctggc catgatcgag gccggcgcca cccgcatcgg cgcgtcgtcg tcggccgcca
     5641 tcctcgacgg catctgagcc ccgcgggcgg cgggctcagc cctccagcga ggcagccagg
     5701 cgctcgaggg tctcgctcat cgtctcgcgg ttgcgcgcct ggcggtcgtc gacaccggac
     5761 atctgcttgc tgaactccag ggccgactcc ggccggaggt cctcactcca ctcggtgacc
     5821 cggcagccgg tgtcggtcgg ctcgatgcgg tagccccagg tcgagaagtg gaagtccatc
     5881 atcgagcact cgaa
//
LOCUS       DATISU010000032        11997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_234316, whole genome shotgun sequence.
ACCESSION   DATISU010000032 DATISU010000000
VERSION     DATISU010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 11997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11997
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234316"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VLR27_01565"
     CDS             complement(<1..1434)
                     /locus_tag="VLR27_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSP02158.1"
                     /translation="MEPTVESSRRIKPHQLALGLGVLIAAVTVVSGIAATYFQFHGDS
                     EITREVFGNIPSPIKAAFYMIVPITLLYGAVAFSQRMKNWERGAPENRRTTKKNVGQR
                     LKDFRAGVYMQTLLRDPAAGVMHSLMYFPFLVLLAVTTVLEVNHQLPDDAKFLHGQVY
                     MAYSFVGDAAGVLFLIGVGWAIVRRYVQRPYRIRMKTKAEYAVILGTLFAIGVTGFLA
                     EMFRIAVDGRPDFEKWSFVGYPLSGLVDNLDAVAGWHQAMWILHVISFLVFLVILPVT
                     MLRHMFTSPINMYLRDRERPKGAMKAMPNLMETELESFGASTVEDFTWKQLVDLDACT
                     MCGRCTSVCPAHATGKPLDPREIVLKSGEVMAATGTPQVSPPLGTVPDITVSANSFFE
                     RITPDEIWACTSCKACDEICPVNIEILDKILDMRRYLSLMESNFPTELGGAYMNMENQ
                     GNPWGMSNATRGDWSKKVEGIEIIEPGD"
     gene            complement(1448..2158)
                     /locus_tag="VLR27_01570"
     CDS             complement(1448..2158)
                     /locus_tag="VLR27_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HSP02159.1"
                     /translation="MATTAAAWATPPRTPVLPDAPAVAVDLDDLDELYPDIDGLDAAD
                     VVLALPEARTLDLLRSRLRGCTIEVPEECSIEAGDSVLDGLDLVGRRITGLVRTHLVR
                     CRLSGADLAEATLEDVTFEDCALDLASWRLARVRRVAVVGGRIDGLDATAAQLEDLTI
                     SGVAMEDVLLERARCARVDLTGADLTSVTAVATLAGATISPAQAVALAARMARSLGVR
                     VLDPDDPARAFPPLRDRK"
     gene            2268..3587
                     /locus_tag="VLR27_01575"
     CDS             2268..3587
                     /locus_tag="VLR27_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSP02160.1"
                     /translation="MTTRRPGPAAAVALVAAVVALCAPLLALAAGASEPEWNIDRIGA
                     PASAAGTVIAVVDTGVDATHPVFAGRVQPAIDLVGGSGGDPNGHGTHVAGTAAGADSG
                     CGAIGVAPDASILPVRVLDEEGTGTADVVAEGIRRAADRGATVINLSLGADVVLRNVS
                     GSALQDAIRYAWAKGSIPVLAAGNDGLLGGLFGSGYGDLPAVVVTATDHRDRAAPYAT
                     SIGSAAWGIAAPGGDGSGTDGRDILSAFPDRRCALHAGTSMAAPHASGALAALRAAGL
                     GPQQAVDRLLGTARDIGSKDTYGHGLIDLRAALDLPPPTPVTTAAAPAAPAPSAAPSP
                     VGSQRPPSTTAAPSTTRGRPAPTSVPASTTTAEPRDVERAEGASPSTTAPAPADGGAA
                     APVVVRDAGGDEAGLPAALVAVAVAAVAGSGVAAGIGARTLGRRPGP"
     gene            complement(3614..4036)
                     /locus_tag="VLR27_01580"
     CDS             complement(3614..4036)
                     /locus_tag="VLR27_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HSP02161.1"
                     /translation="MSKKNASDHLSQVPLFTACSKKELAMIAKATTEMSFPDGTDLTK
                     QDQSAREAFVITEGNVVVKRNGRKVAELGPGSIIGELGLLDKGPRTATVTAQGPVEGL
                     VLGPREFAGLLDEVPSISHKLLKALAARVRELDAKAFG"
     gene            4141..4641
                     /locus_tag="VLR27_01585"
     CDS             4141..4641
                     /locus_tag="VLR27_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP02162.1"
                     /translation="MKDVTIVRWPDERTLREELARSGRARLLMVDTEAAPPIASDPLE
                     DWVRTPCGELDVRARLEGLRLRLERTLELVPELDADGVIRFGDRWAPLPPVEARLTNV
                     LVRRFGAVVSRDDLVAAGWPGGTPGRNALDVHVLRLRRRLEPVRLAIRTVRSRGYLLE
                     PAAAGH"
     gene            4800..6209
                     /locus_tag="VLR27_01590"
     CDS             4800..6209
                     /locus_tag="VLR27_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HSP02163.1"
                     /translation="MKRKLLIGAGLVGAALLLLANPAAAQEAEEVAPASQIVLDNVFV
                     FLCAILVFFMQAGFALVEAGLTRAKNVSNIFMKNLMDGMAGILAFALVGYGIGFGGDE
                     LLGGWFGFGFGITGIEDPAAAQVLNLSPATFFFFQAAFAAVAATIVSGAMAERTKFKS
                     YFIYSLVITAVLYPTVLRWTWGGGWLAQLDIPFSDFAGSTIVHATGGWAALAGAMVLG
                     PRLGKYGKDGKPRAIPGHNVPFVVIGAIILFIGWFGFNPGSELGADNLVPQLAVKTLL
                     AACAGAVVAMIVNWARDGKPDVTMAANGMLAGLVAITAPVGAVETWAAIVIGAIGGAI
                     VVLSVSFFDRMRVDDPVGAISVHGVVGTWGTLSIALFAKYDDAFLGREDAGLFYGGGI
                     DQLSTQALFVGVHFVFVFGAAYLLFQAIKATVGLRVTEEEELAGLDVEEHGHMGYAPD
                     AGGSSSLGTSSSSTKPVSV"
     gene            6232..6570
                     /locus_tag="VLR27_01595"
     CDS             6232..6570
                     /locus_tag="VLR27_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HSP02164.1"
                     /translation="MHLVTAVIKPHKLDDVKEALKGVGVQGMTVSEVKGFGRQGGHTE
                     TYRGAEYTIDFVPKVKIEIVCGTEDAEKVADAIASAAQTGKIGDGKIWMTAVDRLARI
                     RTGEMGPDAV"
     gene            complement(6649..7410)
                     /locus_tag="VLR27_01600"
     CDS             complement(6649..7410)
                     /locus_tag="VLR27_01600"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HSP02165.1"
                     /translation="MSHTLVLLRHGQSVWNLENLFTGWYDAPLTELGEHEARTGGELL
                     LEKGVLPDTVHTSLQTRAIRTAELSLAACDRSWIPVRRHWRLNERHYGDLTGRDKAET
                     AAKYGDDQVHVWRRSYDTPPPAIEADNRFNPNDDPRYAHVPPELRPASECLKDVVERM
                     LPYWFDQIVPDLADGHTVLVAAHGNSLRALVKHLDQISDDEIPGLNIPTGQPLVYELD
                     DDFHPVEAKPVAERYLADPDEVAAAAAAVEAQAKG"
     gene            7518..8207
                     /locus_tag="VLR27_01605"
     CDS             7518..8207
                     /locus_tag="VLR27_01605"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HSP02166.1"
                     /translation="MLDIDEATDHNATAVRLRYLEPGLPLRLAVLSDVLGGFACSIVE
                     TDGRTIWIDLPIRRDAMLDLEAGQLVSVRFDRPGDAVYSFDTVVSDLRADDRAPFGLA
                     MPVTIDRRAHRNDARVPLVLDAHFEVDDGPSGLAKIVDLSAGGVGLICEEELSEGAVL
                     TVRCGLPGPDGSVAIEQRAEVRTVAMYGRTPGGTTLHHYGVAFTGGDDELREQILSSV
                     IWNLTHNPAVL"
     gene            complement(8283..9050)
                     /locus_tag="VLR27_01610"
     CDS             complement(8283..9050)
                     /locus_tag="VLR27_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02167.1"
                     /translation="MTSPAALRPTGSPLRRLPALVAYVPRACLPARRLAVLALPCATI
                     LASGLLSRALDDTAAAAFTDVAALGIFGLAMPLGTLVIGDAVLGAEVRSGTLGFTWLT
                     PVRFWELVLARWLGGWLIALVTLVPCAAIAAVLAGASSLIGPVVVATAAGAAAHIALF
                     VLIGALTRRAAVWSLAIVFVIERLLGAVLSGIAQLSPTWQGQAVFTGLGDVPGAPTRS
                     GIPEGWDAVVRLGLITVVCLALATVGLRRLQLTGARD"
     gene            complement(9047..9997)
                     /locus_tag="VLR27_01615"
     CDS             complement(9047..9997)
                     /locus_tag="VLR27_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP02168.1"
                     /translation="MNETAPGLDPAFVDDATVVVDDVSVWFGQKVALSELSCSFGPGV
                     TGLLGPNGAGKTTLMRAMCGLIPPRRGHVSVLGHDPRGTRDVQARIALVPEEDAVPEA
                     LTARQLVRYQATLNGVTDAGAVERSLAEVSLTDVADRRLGGFSKGMRQRAKVAAALVT
                     DPEVLVLDEPLNGADPVQRVALIELFQALGAAGRTVVVSSHVLHEVERLAERVIVVVH
                     GRLAAAGGHRAIRDAMADRPRRVLLRSDDDRRLATGLLGLDQVVAVELADGGVTISTG
                     NAGELALALPRLSRQLDVRLREVRALDASLDSVFADLTSR"
     gene            complement(9994..10884)
                     /locus_tag="VLR27_01620"
     CDS             complement(9994..10884)
                     /locus_tag="VLR27_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02169.1"
                     /translation="MTVTNPTGRVYDRGYRPYDGELGGRSAARLALFKASVRRALGIR
                     RSWRQKLFPWVLLAIATVPAIVNVGVAYITRDRFTETIEIITYREYVGVSTALLVFVG
                     LTAPDVICPDRRNRVLPLLFSRPLTGSDYVAAKVGAVAAIVFAFGFLPQVVLFVGQML
                     VSDAALDYLTDNAAVLWQVPVAVAVLALYYASLAVAISSFATRRIVAAAALLIVLLVS
                     SVTAAILTEGRTTGTAWAAVNLLNNPLQIRDLIFEGQISPDADLAGVAGGGALALAVY
                     LTVVGASLGTLLWRYRWVET"
     gene            complement(10881..11795)
                     /locus_tag="VLR27_01625"
     CDS             complement(10881..11795)
                     /locus_tag="VLR27_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP02170.1"
                     /translation="MAALLEVDDLTKTYDGTVRALDELAVSLEPGVIGLVGANGAGKS
                     TFFRILLGLTRPDSGTVFVDGIDVGLDPIAVRSRLGYMPEHDCLPTDQSAADVVSMLG
                     EIAGLPARAARQRASDVLDLVGLDEARFRPIEGFSTGMRQRAKLAQALVADPKLVLLD
                     EPTAGLDPQGRHEMLDLVRRLGTFGISVIMATHLLDDVQTTCDQVVMLDAGRLVASGR
                     TEALLHTTGVVRVDTAGRGTDLVAALADRQAVLLADGTVEVPVTGDEDLDAIVAAVAD
                     AGLPLHGLTSQRSSLDDVFLADHAAGVA"
BASE COUNT         1743 a         4133 c         4462 g         1659 t
ORIGIN      
        1 gtcgccgggc tcgatgatct cgatgccctc gaccttcttg gaccagtcgc cgcgggtggc
       61 gttggacatg ccccaggggt tcccctggtt ctccatgttc atgtaggcgc cgccgagctc
      121 ggtcgggaag ttcgactcca tcagcgacag gtagcggcgc atgtcgagga tcttgtcgag
      181 gatctcgatg ttgaccgggc agatctcgtc gcacgccttg cacgaggtgc aggcccagat
      241 ctcgtcgggc gtgatgcgct cgaagaagga gttggccgag acggtgatgt cggggacggt
      301 gccgagcggc gggctgacct gcggcgtgcc ggtggcggcc atgacctcgc cggacttgag
      361 cacgatctcg cgggggtcga gcggcttgcc ggtggcgtgg gccgggcaga cgctggtgca
      421 gcggccgcac atggtgcagg cgtcgaggtc gacgagctgc ttccaggtga agtcctcgac
      481 cgtggaggca ccgaagctct cgagctcggt ctccatcagg ttcggcatgg ccttcatcgc
      541 acccttgggg cgctcccgat ctcggaggta catgttgatc gggctggtga acatgtggcg
      601 cagcatcgtg accggcagga tcaccaggaa cacgaggaac gagatgacgt ggaggatcca
      661 catcgcctgg tgccagccgg cgacggcgtc gaggttgtcg acgaggccgg agagcgggta
      721 gccgacgaag ctccacttct cgaagtcggg gcggccgtcg acggcgatgc ggaacatctc
      781 ggcgaggaag ccggtgacgc cgatggcgaa cagcgtgccg aggatgacgg cgtactcggc
      841 cttggtcttc atccggatgc ggtaggggcg ctggacgtag cggcggacga tggcccagcc
      901 cacgccgatg aggaacagca cgccggcggc gtcgcccacg aacgagtacg ccatgtacac
      961 ctggccgtgg aggaacttgg cgtcgtccgg cagctggtgg ttgacctcga gcaccgtggt
     1021 gacggcaagc agcacgagga acgggaagta catgaggctg tgcatgacgc cggcggcggg
     1081 gtcgcgcagc agcgtctgca tgtacacgcc ggcccggaag tccttgaggc gctggccgac
     1141 gttcttcttg gtggtgcggc ggttctccgg cgcgccccgc tcccagttct tcatgcgctg
     1201 gctgaacgcc acggcgccgt agagcagggt gatcggcacg atcatgtaga acgcggcctt
     1261 gatcggcgac gggatgttcc cgaacacctc gcgcgtgatc tcggagtcgc cgtggaactg
     1321 gaagtacgtg gcggcgatgc ccgagacgac ggtgaccgcc gcgatcagca caccgagccc
     1381 gagggccagc tggtggggct tgatccgacg tgaggactcg accgtgggtt ccatagcggc
     1441 agggaggcta cttgcggtca cgcaggggtg ggaaagccct cgccgggtcg tcggggtcga
     1501 gcacccggac gccgaggctg cgggccatgc gagcggccag cgcgaccgcc tgggccgggc
     1561 tgatcgtggc gccggccagg gtggcgaccg cggtcaccga cgtgaggtcc gccccggtga
     1621 gatcgacccg tgcgcaccgg gcccgctcga gcagcacgtc ctccatggcg acgcccgaga
     1681 tggtgaggtc ctcgagctgc gcagcggtcg cgtccaggcc gtcgatgcga ccgccgacca
     1741 cggccacccg gcggacccgg gcgagtcgcc acgacgcgag gtccagcgcg cagtcctcga
     1801 aggtgacgtc ctcgagggtg gcctcggcca ggtcggcccc actgagccgg caccgcacca
     1861 ggtgcgtgcg caccagaccg gtgatgcgcc gtccgaccag gtcgaggccg tcgagcacgc
     1921 tgtcgccggc ttcgatgctg cactcctccg gcacctcgat ggtgcaccca cgcagccggc
     1981 tgcggagcag atcgagcgtg cgggcctcgg gtagcgccag cacgacgtcg gcggcgtcga
     2041 gtccgtcgat gtccgggtac agctcgtcga ggtcgtccag gtccacggcg accgccggcg
     2101 catcggggag gacgggtgtg cgagggggtg tcgcccaggc ggcggcggtg gtggccaccg
     2161 cggcaccgta ggccgggacc cggacccttc gggccgcccg cgggctttca cccgatttct
     2221 gccgttccgc gtggcatcgc gccaccgtcg ccggtcacca tggggtgatg acgacgcgcc
     2281 gacccggtcc cgctgccgcg gtggccctcg tcgcggccgt cgtcgccctc tgcgccccgc
     2341 tgctggccct tgccgcgggt gcgtcggaac ccgagtggaa catcgaccgc atcggtgcgc
     2401 cggcctccgc ggcgggcacc gtcatcgccg tggtcgacac cggcgtggac gccacccacc
     2461 cggtcttcgc cggccgggtg cagcccgcca tcgacctggt cggcggctcc ggcggcgacc
     2521 cgaacgggca cggcacgcat gtggccggga ccgcagccgg ggcggacagc ggatgcgggg
     2581 ccatcggggt cgcgccggac gcgtcgatcc tgcccgtccg ggtcctggac gaggagggca
     2641 ccggcaccgc cgatgtggtg gccgagggca tccggcgcgc cgccgaccgg ggcgccacgg
     2701 tgatcaacct ctcgctcggt gccgacgtcg tgctgcgcaa cgtgtccggc tccgcgctgc
     2761 aggacgcgat ccgctacgcc tgggcgaagg gctcgatccc cgtgctcgcc gccggcaacg
     2821 acgggttgct cggcgggctg ttcgggtccg gctacggcga cctgccggcc gtcgtggtca
     2881 ccgccaccga ccaccgcgac cgcgccgctc cctacgccac ctcgatcgga tcggcagcct
     2941 ggggcatcgc cgcgccgggt ggggacggca gcgggacgga cggccgcgac atcctctcgg
     3001 ccttccccga ccggcgctgc gctctgcacg ccggcacctc catggccgca ccgcacgcat
     3061 ccggcgccct cgccgcgctg cgggccgccg ggctcgggcc gcagcaggcc gtcgatcgcc
     3121 tgctcggcac cgcccgagac atcggcagca aggacaccta cggccacggc ctgatcgacc
     3181 tccgagcggc actggacctg ccgccgccca ccccggtgac cacggcagcg gcgccggccg
     3241 ctcctgctcc cagcgctgcg ccatcgccgg tggggtcgca gcgccccccg tcgaccacgg
     3301 ccgctccgtc gaccacgagg gggcggccgg caccgacctc ggtcccggcg tcgacgacca
     3361 ccgccgaacc ccgggacgtc gagcgcgcgg agggcgcgag cccgtcgacg accgcgccgg
     3421 cgccggcgga cggtggcgcc gccgcgcccg tggtcgtgcg cgacgcaggt ggcgacgagg
     3481 caggtctgcc ggcagcgctg gtcgccgtcg cggtcgcagc ggtggcgggc agcggggtgg
     3541 cggccggcat cggggcgcgc acgctcggac gtcggcccgg cccgtagccg ccgggccgac
     3601 agctcagctc agctcagccg aaggccttgg cgtcgagctc ccggacgcgg gccgcgagcg
     3661 ccttgaggag cttgtgcgag atggagggca cctcgtcgag caggccggcg aactcgcggg
     3721 gaccgagcac caggccctcg accgggccct gcgcggtgac cgtcgcggtg cggggtccct
     3781 tgtccaggag gccgagctcg ccgatgatcg agcccggtcc gagctcggcg accttgcggc
     3841 cgttgcgctt gaccaccacg ttcccctcgg tgatcacgaa ggcctcgcga gcggactgat
     3901 cctgcttcgt caggtccgtt ccgtcgggga acgacatctc ggtggtggcc ttggcgatca
     3961 tcgccagctc cttcttggag caggcggtga acagcggcac ctgggacagg tgatcggacg
     4021 cgttcttctt cgacatgggc ggagcatacg gaacccccgc ggaccgtgcc gggagaagtg
     4081 ccggagcggt gacctgtggg aaacacggga gaaacggagc gtccgtaccg tccgggagcg
     4141 atgaaggacg tgaccatcgt ccgctggccc gatgagcgga ccctgcggga ggagctggcc
     4201 cggagcggcc gcgccaggct cctgatggtc gacaccgagg cggcgccacc gatcgcgtcg
     4261 gatccgctcg aggactgggt ccgcacgccg tgcggcgagc tcgacgtccg ggcccgcctc
     4321 gaaggcctgc gcctccggct cgagcggacg ctggagctgg tgcccgagct cgacgccgac
     4381 ggcgtcatcc gcttcggcga ccggtgggcg ccgctgccgc ccgtggaggc ccgcctcacg
     4441 aacgtgctcg tccgccgctt cggcgcggtc gtcagccgag acgacctcgt cgccgccggc
     4501 tggcccggcg gcaccccggg ccgcaacgcc ctcgacgtgc acgtgctccg cctccgccga
     4561 cgtctggagc cggtccggct ggcgatccgg accgtccggt cgcgcggcta cctgctcgag
     4621 ccggcggcgg ccggccactg accggagcca ggaaacacgc cggtcacaca gcgttgccaa
     4681 cggcgcaacg accacgagac cgacgtgcaa cgggcggtgc ttaccgtcag ttccgggtca
     4741 gatcgctgtc agccggcagc ccccaccaac ccccagccaa cgaatccaag gagactgctg
     4801 tgaagcgcaa gttgctcatc ggagcaggac tggtcggagc agcgttgctg ctgctggcca
     4861 accctgctgc cgcccaggag gccgaggagg tcgcgcctgc ctcgcagatc gtgctcgaca
     4921 acgtcttcgt cttcctgtgc gcgatcctcg tcttcttcat gcaggcgggc ttcgccctcg
     4981 tcgaggccgg cctcacccgg gccaagaacg tctcgaacat cttcatgaag aacctgatgg
     5041 acggaatggc cggcatcctc gccttcgccc tcgtcggcta cggcatcggc ttcggcggcg
     5101 atgagctgct cggcggctgg ttcggcttcg gcttcggcat caccggcatc gaggacccgg
     5161 ccgctgcgca ggtgctcaac ctgtcgcctg ccaccttctt cttcttccag gcagcgttcg
     5221 ccgccgtggc ggcgacgatc gtgtccgggg ccatggcgga gcgcacgaag ttcaagagct
     5281 acttcatcta cagcctcgtg atcaccgccg tcctctaccc gacggtcctg cgctggacct
     5341 ggggtggcgg ctggctggcc cagctcgaca tcccgttctc ggacttcgcc ggctccacca
     5401 tcgtccacgc cacgggcggt tgggcggccc tcgctggcgc catggtcctc ggcccccgcc
     5461 tcggcaagta cggcaaggac ggcaagcccc gggccatccc cggccacaac gtccccttcg
     5521 tggtgatcgg cgccatcatc ctcttcatcg gctggttcgg gttcaacccc ggctccgagc
     5581 tcggagccga caacctcgtg ccccagctcg ccgtcaagac gctgctggcc gcctgcgccg
     5641 gcgccgtcgt ggcgatgatc gtgaactggg ccagggacgg gaagcccgac gtgacgatgg
     5701 cggccaacgg catgctcgcc ggcctcgtcg ccatcacggc tccggtcggt gcggtcgaga
     5761 cctgggccgc gatcgtgatc ggcgccatcg gtggcgccat cgtggtcctg tccgtgtcct
     5821 tcttcgaccg gatgcgggtc gacgatccgg tcggcgccat ctcggtccac ggcgtcgtcg
     5881 gcacgtgggg cacgctctcc atcgccctgt tcgccaagta cgacgacgcg ttcctcggac
     5941 gggaggatgc cggcctcttc tacggtggtg ggatcgatca gctctccacg caggccctgt
     6001 tcgtcggcgt gcacttcgtc ttcgtcttcg gcgccgccta cctgctcttc caggccatca
     6061 aggccacggt cggcctccgg gtcaccgagg aggaggagct cgccggcctc gacgtcgagg
     6121 agcacggcca catggggtac gcccccgacg ccgggggctc gagctcgctc ggcacctcca
     6181 gctcgtccac caagcccgtc tctgtctgag cgaaccaagg agcacaacgt gttgcatctc
     6241 gtgactgcgg tgatcaagcc gcacaagctc gacgacgtca aggaggccct caagggggtc
     6301 ggcgtccagg gcatgaccgt ctccgaggtc aaggggttcg gtcgccaggg cggccacacc
     6361 gagacctatc gaggcgccga gtacacgatc gacttcgtcc ccaaggtgaa gatcgagatc
     6421 gtgtgcggga ccgaggatgc cgagaaggtg gccgacgcca tcgcctcggc cgcccagacc
     6481 ggcaagatcg gtgacggcaa gatctggatg acggcggtcg atcgcctcgc ccggatccgc
     6541 accggtgaga tgggacccga cgccgtctag gagtcgtccc gacatcaccg cccaacggcg
     6601 ggcctgcacc cccggcttcc ccctgccggg ggtgcggcgc gcccgggctc agcccttggc
     6661 ctgggcctcg acggcggcgg ccgcggccgc gacctcgtcg ggatcggcga ggtagcgctc
     6721 ggccaccggc ttggcctcca ccgggtggaa gtcgtcatcc agctcgtaga cgaggggctg
     6781 gccggtcggg atgttgaggc cggggatctc gtcgtcgctg atctggtcga ggtgcttcac
     6841 cagcgctcgc agggagttgc cgtgggcggc gaccagcacc gtgtggccgt cggcgaggtc
     6901 gggcacgatc tggtcgaacc agtagggcag catccgctcg accacgtcct tcaggcactc
     6961 gctggccggg cgcagctccg gtggcacgtg ggcgtagcgg gggtcgtcgt tggggttgaa
     7021 gcggttgtcg gcctcgatgg cgggcggggg cgtgtcgtag ctgcggcgcc agacgtggac
     7081 ctggtcgtcg ccgtacttgg cggccgtctc ggccttgtcc cggccggtga ggtcgccgta
     7141 gtggcgctcg ttcagccgcc agtgccgacg cacggggatc cacgagcggt cgcacgcggc
     7201 gagggagagc tcggcggtgc ggatcgcccg ggtctgcagg gaggtgtgga cggtgtcggg
     7261 gagcacgccc ttctccagca ggagctcgcc gcccgtccgg gcctcgtgct cgccgagctc
     7321 ggtgagcggg gcgtcgtacc agccggtgaa caggttctcg aggttccaga ccgactgccc
     7381 gtgacggagc aggacgagcg tgtgcgacat ggtcgggacc gtagcgggac cggtcgcgat
     7441 cggtcatccg tccggcaccg catcgaccgg ccggacgggc gtacgattcc cggcagggga
     7501 agaggggggc tcgagacgtg ctcgacatcg acgaggcaac agaccacaac gcgaccgccg
     7561 tccggctgcg gtacctggaa ccggggctgc cgctgcggct cgcggtgctc agcgacgtgc
     7621 tcggcgggtt cgcctgctcg atcgtggaga ccgacggtcg cacgatctgg atcgacctgc
     7681 cgatccggcg ggacgccatg ctcgacctgg aggccggtca gctcgtgtcg gtgcgcttcg
     7741 accggccggg cgacgccgtc tactccttcg acacggtcgt gagcgacctg cgtgccgacg
     7801 accgcgcccc gttcggcctg gcgatgccgg tcaccatcga ccggcgcgcc caccgcaacg
     7861 acgcccgggt cccgctggtg ctcgacgccc acttcgaggt cgacgacggc ccgagcgggc
     7921 tggccaagat cgtcgacctg tccgccggcg gcgtggggct catctgcgag gaggagctgt
     7981 ccgagggagc ggtgctcacc gtgcgctgcg ggctgcccgg tcccgacggc tccgtggcca
     8041 tcgagcagcg ggccgaggtg cgcaccgtgg cgatgtacgg ccgcaccccg ggcggcacca
     8101 cgctgcacca ctacggcgtg gcgttcaccg gtggcgacga cgagctgcgc gagcagatcc
     8161 tgtcgtcggt gatctggaac ctcacccaca accccgccgt cctgtagcga cacccggggg
     8221 agccccggat cgttctgggc cgcggaacac gcctcggagg cgttgctcgc gacccggtcc
     8281 gctcagtcgc gggcgccggt gagctgcagg cgccgcagcc cgacggtggc gagcgccagg
     8341 cagacgaccg tgatgagccc gaggcggacg acggcgtccc atccctcggg gatgccgctg
     8401 cgggtcggag cgccgggcac gtcgccgagg ccggtgaaca ccgcctgacc ctgccaggtc
     8461 ggggacagct gggcgatgcc ggacaggacg gcgccgagca gccgctcgat cacgaacacg
     8521 atggccagcg accacaccgc ggcccggcgg gtgagcgcgc cgatgagcac gaagagggcg
     8581 atgtgggcgg cggcaccggc ggccgtggcc accacgaccg ggccgatgag gctgctcgca
     8641 ccagcgagga ccgcggcgat cgccgcgcag gggacgaggg tgaccagcgc gatgagccag
     8701 ccaccgagcc accgggcgag cacgagctcc cagaaccgga cgggcgtgag ccaggtgaag
     8761 ccgagcgtgc cggagcggac ctcggcgccg aggacggcgt cgccgatgac gagcgtgccg
     8821 aggggcatgg ccagcccgaa gatgccgagc gcggcgacgt cggtgaacgc ggcggctgcc
     8881 gtgtcgtcga gggcccggga gagcaggccg ctggcgagga tggtggcgca cggtagggcg
     8941 agcaccgcga ggcggcgtgc gggcaggcac gctcggggca cgtaggccac gagcgcgggc
     9001 agccggcgca ggggggatcc ggtggggcgg agggcggcgg gggaggtcat cggctggtga
     9061 gatcggcgaa gacgctgtcg agggaggcat ccagggcccg gacctcgcgg agacggacgt
     9121 cgagctggcg ggacaggcgg gggagggcga gggcgagctc gccggcgttg ccggtggaga
     9181 tggtgacgcc cccgtcggcg agctcgacgg ccacgacctg gtcgagcccg aggaggccgg
     9241 tggcgaggcg acgatcgtcg tcggagcgga gcagcacccg gcgggggcgg tcggccatgg
     9301 cgtctcggat cgctcggtgc ccgccggcgg cggcgaggcg accgtgcacg acgacgatga
     9361 cccgctcggc gagccgctcg acctcgtgga ggacgtggct gctcacgacc acggtgcgac
     9421 cggcggcacc gagcgcctgg aacagctcga tgagcgcgac ccgctggacg gggtcggcgc
     9481 cgttgagcgg ttcgtcgagg acgagcacct cggggtcggt gacgagtgcg gcagcaacct
     9541 tggcccgctg gcgcatgccc ttggagaagc cgccgaggcg ccggtcggcc acatcggtca
     9601 gcgacacctc ggccagggag cgctcgacgg cgccggcatc ggtgacgccg ttcagcgtcg
     9661 cctggtagcg gacgagctgg cgggcggtga gcgcctcggg gacggcgtcc tcctcgggta
     9721 cgagcgcgat gcgggcttgg acgtcccggg tgccacgagg gtcgtggccg agcaccgaca
     9781 cgtggccacg ccggggcggg atgaggccgc acatggcgcg catgagggtg gtcttgccgg
     9841 caccgttcgg tccgagcagg ccggtgacgc cggggccgaa cgagcacgac agctccgaca
     9901 gcgcgacctt ctgcccgaac cacaccgaga cgtcgtcgac cacgacggtg gcgtcgtcga
     9961 cgaaggcggg atcgaggccg ggtgcggtct cgttcatgtc tcgacccacc ggtagcgcca
    10021 cagcagcgtg cccagcgacg cgccgacgac cgtcaggtac acggcgaggg cgagcgcgcc
    10081 gccgcccgcc acgccggcga ggtcggcgtc gggggagatc tggccttcga agatgagatc
    10141 gcggatctgc agcgggttgt tcaacaggtt caccgccgcc cacgccgtgc cggtggtgcg
    10201 cccttcggtg aggatcgccg cagtcacgga ggaaacgagc agcacgatca gcagcgccgc
    10261 ggcggccacg atccgccggg tggcgaacga ggagatcgcc acggcgaggg aggcgtagta
    10321 gagggcgagg accgcgaccg cgacgggcac ctgccagagc acggcggcgt tgtcggtgag
    10381 gtagtcgagg gcggcgtcgc tcaccagcat ctggccgacg aagagcacga cctgggggag
    10441 gaagccgaag gcgaagacga tggcggccac ggcgccgacc ttggccgcca cgtagtcgct
    10501 gcccgtcagg ggtctggaga acagcagggg cagcacgcgg ttgcgccgat ccgggcagat
    10561 gacgtcgggt gcggtgaggc cgacgaacac gagcagcgcg gtcgagacgc ccacgtactc
    10621 gcgataggtg atgatctcga tcgtctcggt gaaccggtcc cgggtgatgt aggcgacgcc
    10681 gacgttcacg atggccggca cggtggcgat ggcgagcagc acccacggga acagcttctg
    10741 gcgccacgac ctgcggatgc cgagcgcccg gcgcaccgac gccttgaaca gggcgaggcg
    10801 ggcggcggac cgaccgccga gctcgccgtc gtagggccgg tagccgcggt cgtagacccg
    10861 tccggtgggg ttcgtgaccg tcatgccaca cctgccgcgt gatcggcgag gaagacgtcg
    10921 tcgaggctgg agcgctgact ggtcagaccg tggaggggca gtccggcatc cgcgaccgcg
    10981 gcgacgatgg cgtcgaggtc ctcgtcaccg gtgaccggca cctcgacggt gccgtcggcg
    11041 agcagcacgg cctgccgatc cgccagggcg gcgaccaggt cggtgccgcg acccgcggtg
    11101 tcgacccgca cgaccccggt cgtgtgcagc agcgcctcgg tgcgccccga cgcgaccagt
    11161 cggccggcgt cgagcatcac gacctggtcg cacgtcgtct gcacgtcgtc gagcaggtgg
    11221 gtggccatga tcaccgagat cccgaacgtg ccgagccggc gcaccaggtc gagcatctcg
    11281 tggcggccct gcggatcgag cccggccgtc ggctcgtcga gcagcaccag cttcgggtcg
    11341 gcgacgagcg cctgggccag cttcgcccgc tggcgcatgc cggtggagaa cccctcgatc
    11401 ggccggaacc gggcctcgtc caggccgacc aggtcgagca cgtcgctggc ccgctgccgt
    11461 gccgcccggg ccgggagacc ggcgatctcg cccagcatgg acaccacgtc cgccgccgac
    11521 tggtcggtcg gcaggcagtc gtgctccggc atgtacccga gccgggagcg gaccgcgatc
    11581 gggtccagcc cgacgtcgat cccgtcgacg aagacggtcc cggagtccgg ccgggtcagc
    11641 ccgagcagga tccggaagaa cgtcgacttg ccggcgccgt tcgccccgac caggccgatg
    11701 acgcccggct ccaacgacac ggccagttca tcgagcgctc ggacggtgcc gtcgtaggtc
    11761 ttggtgaggt cgtcgacctc gagcaacgcc gccatccgca gcagtctctc agacagcgag
    11821 tccggcttcc ctcgcgctgg actacctgac aagcccaacc gaggcaagga acggagcggg
    11881 caccgagttt tccgcagtgg gctgcatgcg gagggtccca gcaaacagct tcagacgcct
    11941 accgatgaat tgagcatcct gaggggtggc gactgcgggt gaccgcatct caacatc
//
LOCUS       DATISU010000033        17177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_234326, whole genome shotgun sequence.
ACCESSION   DATISU010000033 DATISU010000000
VERSION     DATISU010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 17177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17177
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234326"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VLR27_01630"
     CDS             <1..502
                     /locus_tag="VLR27_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HSP02171.1"
                     /translation="VLTKNWNPKTGRFDVTFDLPAELDIEQAAVVGEFNDWSESADPM
                     QTDADGRWSATVSLEPSRSYRFRYHLGDDRWENDWNADSYVGNDFGGADSVVTTPEEP
                     SAPPPAKSASPKKAAAKKAAKKAGAAKKSPAKKAATKKAAAGGAAKTTKKAKKAAKKA
                     TKRPPS"
     gene            complement(547..2916)
                     /locus_tag="VLR27_01635"
     CDS             complement(547..2916)
                     /locus_tag="VLR27_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSP02172.1"
                     /translation="MCADVSSLTAFDASAAPVALAEASVVSDRPGWRRALARTELAVV
                     GALAVVALGMAWTAPSVVRVAGAVGVAAMAAAIGLDRDTTVLRGRVVAAFGLTALTVA
                     DAGLPQVDALGDVAVDSLWRPLAAVVAYPVLSRAMILMVGRYRLVRASDVVVEAALVG
                     SAAAIALQVVVNRMSETSGWEPAGAAIASMVVGLDVALAVVIARPLTSAAARRGPVVV
                     VAAAVGGLLIGHLVAAIQIANGVSPGIAATAPIALSLLAIGLGTLYDAAVDRPTIIPV
                     EPPLFSPSHAGIVVLAVIAAPAVLAAQVAWGVSVSGAVAVGAGVICTVLAVHIVSLLQ
                     ERADSEHQATHDVLTGLPNRLLFMDRLERALAHAVRNDVPVGVLYIDLDRFKDVNDTF
                     GHEAGDQLLRETARRLAECARHEDTVARLAGDEFAVLLPHLRSADDVSLVAGRILEAL
                     REPVDLGRFAVHNLASVGAAVFPYDGSDPGDLLSAADAAMYRAKDRGGAKVEMFSATL
                     HEQAATRLELETALHRAILEDELVLYYQPIVEASDGRTSGAESLVRWIHPEKGMIPPN
                     DFIPVAEQSDLIIELGNWVIRAACEQLAELARLGLGDRFVTINVSPRHFREDLVSSIT
                     RALRDTGADPSRLVVELTENAAVDDVQLVAERLRELRQLGIKAAIDDFGTGYCGLQYL
                     GELPVSTLKLDRSFVQAMTPSSAAIVAATIAMAKTLGMNLVAEGVETEDQRKFLVSQG
                     CDRMQGYHLGRPMPAAEFVERLQAEDREFADTRMHEAMGAPAALPSLVD"
     gene            3047..3712
                     /locus_tag="VLR27_01640"
     CDS             3047..3712
                     /locus_tag="VLR27_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP02173.1"
                     /translation="MPIRIVLADDHYLVREGVAALVATEPDLELVATCEDHDQTLVAV
                     EAHRPDVLVTDIRMPPTGTDEGVRIAARLRQDHPGIGVVVLSQYVEPAYALDLLEGGS
                     AGRAYLLKERVSDIDELVAAVRAVAGGGSWIDPKVVEALVSSRSTPADSPLHRLTPRE
                     LEVLSEIAAGKNNAAIAASLVLSGRAVEKHINSIFSKLGLSEETTVHRRVAAVLLFLG
                     DAA"
     gene            3788..4168
                     /locus_tag="VLR27_01645"
     CDS             3788..4168
                     /locus_tag="VLR27_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP02174.1"
                     /translation="MSPDTPVLVVDDQAPFRAIARQVVDLTPGFEVVGEAESGEDAVL
                     LAHRLAPAIVLMDINLPGINGIEATRRILADAPSVRVILLSTYRESDLPADALACGAR
                     RYVHKEDLEPGVLLDVAGDQPVSD"
     gene            complement(4169..6292)
                     /locus_tag="VLR27_01650"
     CDS             complement(4169..6292)
                     /locus_tag="VLR27_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSP02175.1"
                     /translation="MTTASAPAVLPAPAPSSRSSLAPVPLVLVPLAFTAAAITVLVGD
                     DRLLAADVVRVVLVVAFAGAVRALWRQVPLRTLALLVSAGLTVAAVEFLCAALLRSDR
                     TSFVEAVESMAAPLVLAVAFHGLATLPDGRIRRPAQRACVVAAYVAAALAGGIRWADQ
                     PEPSAWPLHLLGAALVVAGLTLSHRSYRVARGAARQRMQWLGLALSVVAEAAVVVGAV
                     HLLLDWPADAGVVVGASLVVVAGALAASASPRHVGRVDRLLTHAVSLAGLTAVVVAVY
                     LVVVVGLGRIPTDSERSVLVLSMLAAAVSALLYLPARDRLTIAADRLVYGEPQDPAQA
                     LDTFGSRMTRALPMEELLLQLAELAKKHFSLRAAEVWTGVDGRLVRAASVPDVGAGAL
                     RLGTSELPVVTRAGVSGRGWAAVWLPQVLEGRGVGPLRIAPMTHSGQLFGLLVLERPD
                     GDDEFSEEDDRVLTELARQVGLALQNSELDNALKATLEQVQRKNAELQESRARIVATG
                     DAERRKIERNLHDGAQQHLVALAVKLRLIQRLAEADTQQALGMLEEARNDVLATVEEV
                     RALAHGIYPPLLMDRGLPEALRAAAGRAPLPTTVAADGVDRYGQDAEAAVYFCCLEAI
                     QNAGKHAGDGATVTVELAAGDEELRFTVVDDGAGFDLAGGVRGQGFVNMADRLGAIGG
                     SVTVWSAPGEGTRITGRLPALPRSA"
     gene            complement(6342..8675)
                     /locus_tag="VLR27_01655"
     CDS             complement(6342..8675)
                     /locus_tag="VLR27_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSP02176.1"
                     /translation="MAVLVGVGGGLAMGSLAAARRTDTAYPRFLEQARPPDLVLDPDF
                     EADSAGFLDAVEELPAVADRSDAQAVAMARVVEGQLQLDDFGTTIASVDGERFYERDR
                     VHVVEGRMPDPERVDEVLVSHTVAAEGLAVGDHLTYGVLDVQEAFGALEQGRSEFTED
                     DAVRVVEVEVTGIGIFPELAVTEEVLAPDVVLITPALFRELPAEAALWTRAGIHLEPG
                     ADAGAVQRQIKDLAAASGGDTFFEDRADITDRAQRSLRPYVLALTGLGVAGAVFVTLL
                     ATQLVRRTVAQADADRRVLVALSAGPRLVRAGAALPAAVAAVLAVGVALAVQAGVSAG
                     TPVGPVESIEPSPGVHLDWVVAATGAVLLLAVCLLPALAVTQRSQPARVPAGALTTWA
                     THAGAPLPLLLGIGRATGLGDRWRRRAARSGLAAVALAATMLVAVATFAASLAHLVDH
                     PEVHGWNADVGLLGAGGYGSFDLEAAAEVEGVESITGGVFANVGIEDTTVPGVGLVPL
                     RGGLLPPVLEGRAPQAPGEIALGQASLAEIGARVGDVVDVRTPGDEPTAMTIVGTTVL
                     PGLGPIDNDRPALGNGAFIVFPPDSIGEATWSLILADLAPGADRRTVVDDLVLAADEQ
                     AGETDVFDVFRPADIDAFADVGAVPLALVGLFGLVALGSLVHVLFVTAVAWRRDRAVL
                     AALGATPRQLRESVRWQSVVVVALALVAALPAGSVLGRRAWQSLATEVGVVPDTVVPT
                     TFLAALVVALLLVALALTAVPERRAAGQRPVDHLRAD"
     gene            complement(8789..11161)
                     /locus_tag="VLR27_01660"
     CDS             complement(8789..11161)
                     /locus_tag="VLR27_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP02177.1"
                     /translation="MLLTLLVALVVGAAGGAFAGGRRASSSLDRFLAYNRPPDAQVYT
                     PDGGSFDVGAVTALPHVAGWSTGAYGLLTAEGPGGGPFPSGDVNPFIPIAVDGARSYR
                     PFVVDGRLPDDGDPHELALDEEAARRLGAAVGDAVELRLYTPSQMESFFDGGDFPPPD
                     GATVATEVTAIVRHAFDVTPSKPEDIDAVQLASSEVYPSLAFWDRYGDGMAAYGDGTD
                     NVEVVLSGGAGAVHAFEEAIRALPGGDSLVVDVANDALVAVDDARQAVRFEAFAALLF
                     GALVLVVGVVLAGQAIARQVRAELDQGAVLRGIGLTRSDIARAATLRMSAVVLGGSAL
                     GVGLAYLSSARMPFGTAAKAEIEPGLRLDLLPLAGAAAVVAVSLLLWSARASWHAEGR
                     PTPVRGGWAERLAVAAATGGAPVSVVFGLSQLGRARVGSPVRSSMSGVTFAVAAVVAV
                     LVYGATTSRFVEEPDQHGWAWDLLVGDSDDEKLVESGAQRLEETDGVVGFASLWTGWE
                     DTVSTSADRQVVITAVERGAGDTYIQLADGRSADRVDEVVLGRQTMEELDVSLGGRVL
                     LEGPGGGGDYRVVGVAVLHQAVSDGFELDEGAVTSREGLARLFGDDMVLDRFLVDVDP
                     GADPPSTMASLREDFGPTVTPHVPPLDVASLQGTQRLPVLLGLVVAMIGAGSLVHLLL
                     VVVRRRRIEFAVLRSLGATPGQVSSAVATVASAAGAIAICIGVPAGVVGGRVAWTALA
                     GSVGAPTAPVVPISTVLVGTVAMVVLANVIAAVPGRLVRRTAPAEILRSE"
     gene            complement(11246..11983)
                     /locus_tag="VLR27_01665"
     CDS             complement(11246..11983)
                     /locus_tag="VLR27_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP02178.1"
                     /translation="MTVHVGGIGDPVLEIGGVRKTFEADQAPVRALRGLDFEMARAEF
                     VAVMGPSGCGKSTMLNLVAGLDAVDEGTVELDGEQIHAMDEDDLARMRRRHIGIVFQF
                     FNLLEGMTVLENVALPAMIAGLKRKPAETRARDLLDLLGLSDKARQLPGVLSGGQRQR
                     LAIARALANEPTLVLADEPTGALDSDGGQEILELFRRLHAEGQTILMVTHDDDVAAAA
                     DRIVRMRDGRVEVPEAEPSATPAPVLL"
     gene            complement(11980..13338)
                     /locus_tag="VLR27_01670"
     CDS             complement(11980..13338)
                     /locus_tag="VLR27_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSP02179.1"
                     /translation="MIETTEAGGVRSAISVFLADDNLIVREGVRALLGLEPDLEVVGV
                     ADDFDSLVAGAEERNPQVLVTDIRMPPSFQQEGIEAAKLIRKRHPGTGVVVLSQFDDP
                     EYAVSLLNDGAAGYAYLLKDRVAEGDHLAAAIRTVATGGSMLDPEIVQALVTPVSSDG
                     ALRPADEALLQMVAEGKPIKAIAAMQQTTPAIVAEAVDAMFLRLARGARQGSDPALRR
                     LRMLHQAIVDREEQGESLSRLLPGGVAEQVRAGGMVIGESERMVVTVLMADIRGYSTI
                     AERVDPTALAGQLNEHRAAMNRAILGNDGTVMQFVGDAVMAVFGAPLPCPDHPDRAVA
                     AARAMHDAQDEINERWEEQGLPPFGLGIGVSTGEVAAALLGSEERLEYTVVGDAVNIT
                     QRLQQWAEAGETVLTDATWRTLRTAVAADALEPAVVKGRDTPVQAYKVPAVRRAGTGV
                     GA"
     gene            complement(13438..14238)
                     /locus_tag="VLR27_01675"
     CDS             complement(13438..14238)
                     /locus_tag="VLR27_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02180.1"
                     /translation="MPTTTRSRRVKALAAVCAFGLLATACGDDDDAETSTADDPATTL
                     AEGEAADTDVEEYCAAIEELDSQDGPPTEEQLLELKEIRPESIGEETDMVVDAFIAAD
                     GDMGAVFSDPAIEEAFGAMEAHDAEVCGFEASEDEEEEAATEPLDGAEVIPVTGIDFA
                     FEGLPDTVPAGPVSFEFTNEGDAAHEMAVFKLGEGVDMDELLASEQEPTEEEAQEVGF
                     TFAPPGEGGVYLNAEELTPGTYAVVCFIPGPEGKAHYELGMKQTFQVG"
     gene            14398..15408
                     /locus_tag="VLR27_01680"
     CDS             14398..15408
                     /locus_tag="VLR27_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HSP02181.1"
                     /translation="MRIDGSVTSVSWIPSEAVKGIMKLPFSMGVAHYDDPPPERLPGA
                     SVPSALDELRRADRFRFANHLTAWVEVDAGRIVRWGQGGEPMIGSTTMRAGRSLTVAA
                     VAFEPIRPEPEVGEGWVRFRQSAGGRTGVPAPRHVSRPPFVQVSAPSAWTTLELTLHA
                     DGRQELGLAGASPFPRHWLYDGAGMLVRKSGMIDFKDWYRRAFGTHSPWGDEDSPALA
                     TEVETALERQLSTTIMRGGTRPRIRKVVAGDALVQQGDEGEELFLLLDGVLAVEVDGE
                     RLAELGPGAVVGERALLEGDLRTSTLTALTPCKVAVARADELEPAALGALRETHRRED
                     AR"
     gene            15413..16249
                     /locus_tag="VLR27_01685"
     CDS             15413..16249
                     /locus_tag="VLR27_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSP02182.1"
                     /translation="MRLHLLGVRGSTPTPGPAFARHGGHTSCVAVEAEDGSVPIVLDA
                     GTGLRSLGRVLDGRAFVGTLLLGHLHWDHTQGLPFSPVVDHPDARVRVLAPTACGQSV
                     DQLLAQAMSPPNFPIRPDELRGDWSWGPLVEGHRSLEGFEVLAREIPHKGGRTFGFRV
                     SDGTRAFAYLSDHMPQALGPGPDGLGARHDAALALADGVDVLIHDAQYTAEELPTRGA
                     FGHSATEYAVALGQEAGAGRVVLFHHDPQRTDDEIDAIVARFTDAPMPVVAAVEGATI
                     SC"
     gene            16318..>17177
                     /locus_tag="VLR27_01690"
     CDS             16318..>17177
                     /locus_tag="VLR27_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP02183.1"
                     /translation="MPTHDDVDLGLGSMIERRTHLTPNLPALTFEGTTWTYAEMLDRI
                     DRFATVLADGGISRGDRVAYLGLNHANFFITLFATARLGATFVPLNFRLTGPELSFIV
                     GDADVHTVVADDMHRPVIDSIRDDIPCQVHLSSETAADGWPAMDDLAAGAAPLTDPVR
                     AAQGDVAVIMYTSGTTGRPKGAMLTHANIAWNNVNALHAIDLGADEVALVVAPLFHIG
                     GLNVTTLLVWQKGGHIVLHRAFDPAAALRAIEEHGITNMFGVPAMYLFMSQVPGFDQA
                     DLSSVRVFVVG"
BASE COUNT         2486 a         6324 c         6194 g         2173 t
ORIGIN      
        1 cgtgctcacc aagaactgga atccgaagac cggacgattc gacgtcacct tcgacctccc
       61 ggccgagctc gacatcgagc aagccgcagt cgtcggtgag ttcaacgact ggtccgagtc
      121 ggccgacccg atgcagaccg atgccgacgg tcgctggtcg gccaccgtgt ccctggagcc
      181 gagccggtcg taccgcttcc gctaccacct gggcgacgat cggtgggaga acgactggaa
      241 cgccgacagc tacgtcggca acgacttcgg tggcgccgat tcggtggtga ccaccccgga
      301 ggagccgagc gctcccccgc ccgccaagtc ggcgagcccg aagaaggccg ccgccaagaa
      361 ggcggcgaag aaggccggcg cggccaagaa gtccccggcg aagaaggcgg ccacgaagaa
      421 ggccgcggcc ggtggcgctg cgaagacgac caagaaggcc aagaaggcgg cgaagaaggc
      481 caccaagcgc ccaccgagct gatcgcttct ggcagcggtg gcggaacagc gcccgctgcc
      541 gcgaggtcag tcgacgagtg agggcagcgc cgctggcgcg cccatcgcct cgtgcatccg
      601 cgtgtcggcg aactcgcggt cctcggcctg cagtcgctcg acgaactcgg cggcgggcat
      661 cggccgaccc aggtggtacc cctgcatgcg gtcacagccc tgggacacga ggaacttgcg
      721 ctggtcctcg gtctcgacac cttctgccac caggttcatc cccagggtct tggccatggc
      781 gatggtggcg gcgacgatcg ccgcggagct cggggtcatg gcctggacga aggaccgatc
      841 gagcttgagg gtgctcactg ggagctcgcc caggtactgc aggccgcagt agccggtgcc
      901 gaagtcgtcg atggcggcct tgatgcccag ctgacggagc tcgcggaggc gctcggcgac
      961 gagctgcacg tcgtccacgg cggcgttctc ggtcagctcg acgaccagtc gggacgggtc
     1021 ggcgccggtg tcgcgcagcg cccgggtgat cgaggagacc aggtcctccc ggaagtggcg
     1081 agggctgacg ttgatcgtga cgaagcggtc cccgagcccg aggcgggcca gctcggcgag
     1141 ctgctcgcag gcggctcgga tgacccagtt ccccagctcg atgatgaggt cggactgctc
     1201 ggccaccgga atgaagtcgt tcggcgggat catgcccttc tcgggatgga tccagcggac
     1261 cagcgactcc gcgccgctgg tgcgcccgtc ggaggcctcg acgatcggct ggtagtagag
     1321 caccagctcg tcctcgagga tggcgcggtg cagggcggtc tcgagctcga gccgggtcgc
     1381 ggcctgctcg tggagggtgg cgctgaacat ctcgaccttg gcgccgccgc ggtccttggc
     1441 tcggtacatg gcggcgtcgg ccgcgctgag caggtccccg gggtcgctgc cgtcgtaggg
     1501 gaagacggcc gcaccgacgc tggccaggtt gtgcacggcg aagcggccga gatcgaccgg
     1561 ctcccgcagc gcctccagga tgcgtccggc caccaggctg acgtcgtcgg cggatcggag
     1621 gtgcgggagc agcacggcga actcgtcgcc ggccaggcgg gccacggtgt cctcgtggcg
     1681 ggcgcactcg gccagtcgcc gggcggtctc gcgcagcagc tggtcaccgg cttcgtggcc
     1741 gaaggtgtcg ttcacgtcct tgaagcgatc gaggtcgatg tagaggacgc cgaccgggac
     1801 gtcgttgcgg acggcgtggg ccagggcccg ctcgaggcga tccatgaaca gcaggcggtt
     1861 gggcaggccg gtgagcacgt cgtgggtggc ctggtgctcg ctgtcggccc gctcctgcag
     1921 gaggctcacg atgtgcacgg cgagcaccgt gcagatgaca ccagcgccca cggcgaccgc
     1981 gccggagacc gagacccccc acgccacctg ggcagccagg accgcggggg cggcgatgac
     2041 agccaggacg acgatcccgg cgtgcgacgg ggagaacagc ggcggttcga ccgggatgat
     2101 cgtcggccgg tcgacagcgg cgtcgtagag cgtgccgaga ccgatggcca gcagcgacag
     2161 ggcgatgggg gcggtggcgg cgatgccggg gctgaccccg ttggcgatct ggatggcggc
     2221 gaccaggtgg ccgatcagca ggccgccgac tgcggcggcg acgaccacga cggggccacg
     2281 acgagcggcg gccgacgtga gcggtcgggc gatgacgacg gccagcgcca cgtcgaggcc
     2341 gaccaccatg gaggcgatgg ccgccccggc cggctcccat ccgctcgtct cggacatgcg
     2401 gttgaccacg acctggaggg cgatggcggc ggccgacccg acgagggcgg cttccacgac
     2461 gacgtcgctc gctcggacca gccggtagcg accgaccatg aggatcatcg cccggctgag
     2521 caccgggtag gccaccacgg cggcgagcgg tcgccacagc gagtccacgg cgacgtcgcc
     2581 gagcgcgtcg acctgcggca ggccggcatc cgcgacggtc agggcggtca ggccgaaggc
     2641 ggcgacgacc cgaccccgca ggaccgtcgt gtcccggtcg agtccgatgg cggcagccat
     2701 ggcggcgacg ccgacggcac cggccacccg cacgaccgac ggcgcggtcc acgccatccc
     2761 gagggcgacc acggcgaggg cgcccacgac cgccagctcg gtgcgggcca gggcgcgtcg
     2821 ccagcccggc cggtccgaga ccacggaggc ctcggccagg gcgacgggcg ccgcggatgc
     2881 atcgaaggca gtcaggctgg agacatcggc acacacggca ccggcctgaa tggcccgatg
     2941 gggccattct cgtcaaccca acatcgtgcc gccgccgccg agatgcccgg ttcggggcat
     3001 gtgcgcggtc accggcgcgc cgccgggctc acatagcctc cccccggtgc cgatccggat
     3061 cgtgctcgcc gacgaccact acctggtccg cgaaggtgtc gccgccctcg tcgcgaccga
     3121 gcccgacctc gagctggtgg ccacctgcga ggaccacgac cagacgctcg tcgccgtcga
     3181 ggcccaccgg cctgacgtgc tggtcaccga catccggatg ccgccgaccg gcacggacga
     3241 gggcgtgcgg atcgcggccc gtctgcgcca ggaccacccc ggcatcggcg tggtggtgct
     3301 gagccagtac gtcgagcccg cctacgcgct cgacctgctc gaaggcggct ccgccggtcg
     3361 cgcctacctg ctcaaggagc gggtgtccga catcgacgag ctggtcgcag ccgtgcgggc
     3421 cgtcgccggc ggggggtcct ggatcgaccc gaaggtggtc gaggctctcg tgtcctcccg
     3481 gagcaccccg gccgactcgc cgctgcaccg cctgacgccg cgcgagctcg aggtgctgtc
     3541 cgagatcgcc gccggcaaga acaacgcggc gatcgcggcg tcgctcgtgc tgtccgggcg
     3601 cgccgtggag aagcacatca actcgatctt ctcgaagctg ggcttgtccg aggagaccac
     3661 ggtgcaccgc cgggtcgccg ccgtgctgct gttcctcggc gatgcagcct gagggtgctg
     3721 gcaccaccac cgcgccaccg gcgcaccccc tccccggacc gtgcggggca tgggacgatg
     3781 tcgccagatg agccccgaca cgcccgtcct cgtcgtcgac gaccaggcac ccttccgcgc
     3841 catcgcgcgg caggtcgtcg atctgacccc cgggttcgag gtcgtcgggg aggccgaatc
     3901 gggtgaggac gccgtcctgc tggcccatcg cctggcgccg gcgatcgtgt tgatggacat
     3961 caacctgccg gggatcaacg ggatcgaagc gacccggcgg atcctggccg atgctccgtc
     4021 cgtccgggtg atcctgctgt cgacctaccg ggagagcgac ctcccggccg acgcgctggc
     4081 ctgcggcgcc cgccggtacg tccacaagga ggacctcgag cccggggtcc tgctggatgt
     4141 ggccggcgac cagccggtca gcgactgatc acgccgatcg gggcagggcc ggcagccgac
     4201 cggtgatgcg ggtgccctcg cccggcgccg accagaccgt caccgacccg ccgatggcac
     4261 cgagccggtc ggccatgttg acgaagccct ggccccgcac cccgccggcg agatcgaagc
     4321 cggcgccgtc gtcgaccacg gtgaagcgca gctcctcgtc cccggcggcg agctccaccg
     4381 tgacggtcgc cccatctccc gcgtgcttgc cggcgttctg gatcgcctcg aggcagcaga
     4441 agtacacggc cgcctccgcg tcctgcccgt agcggtcgac gccgtccgca gcgacggtgg
     4501 tgggcagcgg cgcgcgacca gcggcggccc ggagcgcttc ggggaggccg cggtccatca
     4561 gcagcggcgg atagatgccg tgggcgagcg ctcgcacctc ctcaacggtg gcgagcacgt
     4621 cgttgcgggc ctcctcgagc atgccgagcg cctgctgcgt gtccgcctcg gccagccgct
     4681 ggatgagtcg cagcttcacg gcgagggcga cgaggtgctg ctgggcgccg tcgtgcaggt
     4741 tccgctcgat cttgcgtcgt tcggcgtcgc cggtggccac gatccgggcg cgcgattcct
     4801 ggagctcggc gttcttgcgc tgcacctgct cgagggtcgc cttcagggcg ttgtccagct
     4861 cgctgttctg cagggccagg ccgacctggc gggcgagctc ggtgaggacg cggtcgtcct
     4921 cctccgagaa ctcgtcgtca ccgtcgggcc gttcgagcac cagcaggccg aagagctgcc
     4981 cggagtgggt catcggcgcg atccgcaacg ggccgacccc ccggccctcc agcacctgcg
     5041 ggagccacac cgccgcccag ccgcgcccgg acacgccggc cctggtcacg acggggagct
     5101 cggacgtgcc caaccggagc gcaccggccc cgacgtccgg cacggaggcc gcccggacga
     5161 ggcgaccgtc gacgccggtc cacacctccg ccgctcgcag cgagaagtgc ttcttcgcca
     5221 gctcggccag ctggagcagc agctcctcca tcggcagggc tcgcgtcatg cgggagccga
     5281 aggtgtcgag cgcctgcgcc gggtcctgcg gttcgccgta gacgagccgg tcggcggcga
     5341 tggtgaggcg atcgcgcgcc gggaggtaga ggagcgccga caccgcggca gccagcatcg
     5401 acagcacgag gaccgagcgc tcgctgtcgg tcgggatccg gccgagcccc accacgacga
     5461 cgaggtagac cgccaccacg acggcggtca acccggcgag cgagacggca tgggtcagca
     5521 gccggtcgac ccgccccacg tgccgcggcg atgccgatgc ggccagcgcg ccggcgacca
     5581 cgacgaggct cgctccgacc accacgccgg cgtccgccgg ccagtcgagg agcaggtgca
     5641 cggcgccgac gacgacggcc gcctccgcga ccacggacag ggccaggccg agccattgca
     5701 tccgctggcg tgcggcgccg cgggcgaccc ggtacgaccg gtgggacagg gtgaggccgg
     5761 ccacgacgag cgcagcgccg aggaggtgga ggggccacgc cgacggctcg ggctggtcgg
     5821 cccagcggat cccgccggcg agcgcggcgg ccacgtacgc cgcgacgacg caggcacgct
     5881 gcgccggtcg gcggatgcgt ccgtcgggca gggtggcgag accgtggaag gccacggcca
     5941 ggacgagggg cgccgccatg ctctccaccg cctcgacgaa ggacgtccgg tcggaccgca
     6001 ggagcgcggc gcacaggaac tcgacagcgg ccacggtcag cccggcggac accaggagcg
     6061 ccagcgtgcg gagcggcacc tggcgccaga gcgcacggac cgctccggcg aaggcgacca
     6121 ccagcaccac gcgcacgacg tcggccgcca gcaggcggtc gtcgccgacc aggacggtga
     6181 tcgctgcggc ggtgaacgcc agcggcacga gcacgagcgg caccggcgcc agcgacgaac
     6241 ggctcgacgg ggcaggggcc gggagcacgg cgggtgcgga tgcggtcgtc atcgtgcagg
     6301 tctcctgggc agcggcgcgg tggggcgggc aggcgcggac atcagtcggc ccgcaggtgg
     6361 tcgacggggc gctggccggc cgctcgtcgc tccggcacgg ccgtcagcgc gagggcgacc
     6421 agcagcaggg cgacgacgag tgcggccagg aacgtcgtcg gcacgacggt gtccggcacg
     6481 acgccgacct ccgtggccag tgactgccag gcccgccgac ccagcaccga gccggcgggc
     6541 agggcggcga ccagggcgag cgcgaccacg accaccgact gccaccggac cgactcccgg
     6601 agctggcgcg gcgtggcacc gagggccgcg aggacggcgc ggtcccgccg ccaggcgacg
     6661 gcggtgacga acaggacgtg gacgagcgag ccgagggcga ccaggccgaa gagcccgacc
     6721 agcgccagcg ggacggcgcc cacgtcggcg aaggcgtcga tgtcggctgg ccggaagacg
     6781 tcgaagacgt ccgtctcgcc cgcctgctcg tcggcggcga gcacgagatc gtccacgacg
     6841 gtgcgccggt cggcgcccgg cgcgaggtcg gcgaggatca ggctccacgt cgcctcgccg
     6901 atgctgtccg gcgggaagac gatgaaggca ccgttcccga gcgccggacg gtcgttgtcg
     6961 atcggtccca acccgggcag gacggtcgtg ccgacgatcg tcatggccgt cggctcgtcg
     7021 ccgggggtgc ggacgtcgac gacgtcgccg acccgagcac cgatctcggc cagcgacgcc
     7081 tggccgaggg cgatctcacc tggtgcctgc ggggcccgac cctcgaggac cggcggcagc
     7141 agcccgcccc gcagcggcac caggccgacg cccggcacgg tggtgtcctc gatgccgacg
     7201 ttggcgaaca ccccgccggt gatggactcg accccctcga cctccgccgc cgcctccagg
     7261 tcgaagctcc cgtagccgcc ggcacccagc agcccgacgt cggcgttcca gccgtgcacc
     7321 tccgggtggt cgacgaggtg agcgagcgag gccgcgaagg tggcgaccgc gacgagcatg
     7381 gtggcggcga gggcgacggc ggcgaggccc gagcgagccg cccggcgccg ccagcgatca
     7441 ccgaggccgg tggcacgtcc gatgccgagc agcagcggca gcggcgcacc ggcgtgcgtg
     7501 gcccaggtgg tcagcgcccc ggccgggacc cgggcgggct gcgaccgctg ggtcacggcg
     7561 agcgccggga gcaggcagac ggcgagcagc aggacggcgc cggtcgccgc gacgacccag
     7621 tcgaggtgga cgcccgggct cggctcgatg gactccaccg gacccaccgg tgtcccggcg
     7681 gagaccccgg cctggacggc gagcgcgacg ccgacggcga ggacggcagc gaccgctgcc
     7741 gggagcgccg cgccggcccg caccaggcgg gggcctgcgc tcagcgccac cagcacgcgg
     7801 cgatcggcgt cggcctgcgc gaccgtccgg cggacgagct gggtggccag cagcgtgacg
     7861 aagaccgcac cggcgacccc gaggccggtg agggcgagga cgtacgggcg cagcgaccgt
     7921 tgggctcggt cggtgatgtc ggcccgatcc tcgaagaagg tgtccccgcc gctggcggcg
     7981 gcgaggtcct tgatctgtcg ctgcaccgcc ccggcgtcgg cccccggctc gaggtggatg
     8041 ccggcacggg tccacagcgc agcctccgcc ggcagttccc ggaagagcgc aggcgtgatc
     8101 agcacgacgt cgggcgccag cacctcctcg gtgacggcga gctccgggaa gatgccgatc
     8161 ccggtgacct ccacctcgac gacccgcacc gcgtcgtcct cggtgaactc ggaccgcccc
     8221 tgctccagcg cgccgaaggc ctcctggacg tcgaggacgc cgtacgtgag gtggtcgccg
     8281 acggccagcc cctccgccgc gaccgtgtgg ctgacgagca cctcgtcgac gcgctccggg
     8341 tccggcatcc ggccctcgac gacgtggacg cggtcgcgct cgtagaagcg ctccccgtcg
     8401 acgctggcga tcgtcgtccc gaagtcgtcc agctgcagct gcccctccac cacgcgcgcc
     8461 atcgccacgg cctgggcgtc ggagcgatcg gcgacggccg ggagctcctc gacggcatcg
     8521 aggaacccgg cgctgtcggc ctcgaagtcg ggatcgagca ccaggtccgg cgggcgggcc
     8581 tgctcgagga accgggggta ggcggtgtcg gtccggcgtg ctgcagcgag gctgcccatg
     8641 gcgaggcccc cgccgacgcc gacgaggacg gccaccgaga gccacgctcg ccaacggcga
     8701 cgcagctcgg cccgggcgca gtaccaggcg acagcgatcg tcgtcgtgcg tcctgcaacc
     8761 ggatgatcgg aggcgaggcg ggtcaccctc actccgaccg gaggatctct gccggcgcag
     8821 tccggcggac gagccggccc ggcaccgcgg cgatgacgtt cgcgaggacg accatggcaa
     8881 cggttccgac gagcaccgtc gagatcggga cgaccggtgc cgtcggtgcg ccgacgctgc
     8941 cggcgagggc ggtccacgcg actcgaccgc ccacgacgcc tgcgggcaca ccgatgcaga
     9001 tggcgatggc gccggcggcc gacgccacgg tcgcgaccgc ggacgacacc tggcccggcg
     9061 tcgcgccgag cgagcgcagc actgcgaact cgatgcgacg acgacgcacg acgacgagca
     9121 gcaggtggac gagggaaccg gcgccgatca tcgcgaccac gagcccgagc agcacgggaa
     9181 gccgctgggt tccctgcagg ctcgccacgt cgagcggggg cacgtggggc gtgaccgtgg
     9241 gcccgaagtc ctctcggagc gacgccatcg tcgatggcgg gtccgcgccc ggatcgacgt
     9301 cgacgaggaa ccggtccagg accatgtcgt caccgaagag gcgggcgagg ccctcccggg
     9361 acgtgaccgc accttcgtcg agctcgaagc cgtcgctgac ggcctggtgc agcacggcga
     9421 cccccaccac tcggtagtcc ccgcctccgc cgggcccttc caacagcacc cgcccgccga
     9481 gggagacgtc gagctcctcc atcgtctggc gcccgagcac cacctcgtcg actcgatctg
     9541 cggatcgccc gtcggccagc tggatgtagg tgtcaccggc cccacgctcc accgccgtga
     9601 tgaccacctg acgatcggcg gatgtgctga cggtgtcctc ccaccccgtc cacagggaag
     9661 cgaagcccac caccccgtcc gtctcctcga ggcgctgggc cccgctctcg accagcttct
     9721 cgtcgtccga gtcgcccacc agcaggtccc acgcccaccc gtgctgatcg ggttcctcca
     9781 cgaaccggct cgtcgtcgcg ccgtacacga gcaccgcgac cacggcagcg accgcgaacg
     9841 tgacaccgct catcgagctc cggacggggg acccgacccg cgcccggccg agctgggaca
     9901 gcccgaagac caccgagacc ggtgccccac cggtcgccgc agcgacggcg agccgctccg
     9961 cccacccccc acgcaccggt gtcggacgac cctctgcgtg ccaggacgcc cgagccgacc
    10021 acagcaggag cgacaccgcc accaccgccg ccgccccggc gagcggcaac aggtccagcc
    10081 ggaggccggg ctcgatctcc gccttcgccg ccgtgccgaa cggcatccgg gcagaggaca
    10141 ggtaggccag tcccacgccg agggcgctgc caccgagcac gacggcggac atccggagcg
    10201 tggcagcgcg ggcgatgtcg cttcgggtca acccgatccc gcggagcacg gcgccctggt
    10261 ccagctccgc tcgcacctgg cgcgcgatcg cctgaccggc gagcaccacg ccgaccacga
    10321 gcacgagggc accgaagagc agcgcggcga acgcctcgaa gcggacggcc tgccgtgcgt
    10381 cgtcgacggc gaccagcgcg tcgttcgcga cgtccacgac cagggagtcg cctcccggca
    10441 gcgcccggat cgcctcctcg aacgcgtgga cggcaccggc accgcctgac aggacgacct
    10501 ccacgttgtc cgtgccgtcc ccgtacgccg ccatcccgtc gccgtaccgg tcccagaacg
    10561 cgagcgacgg gtagacctcg gaggacgcca gctgaacggc gtcgatgtcc tccggcttcg
    10621 acggcgtcac gtcgaaggcg tggcgcacga tcgccgtcac ctccgtggcg acggtcgctc
    10681 cgtccggtgg cgggaagtca ccgccgtcga agaacgactc catctggctc ggcgtgtaga
    10741 ggcggagctc gaccgcgtcg cccactgcgg cgccgaggcg tcgtgccgcc tcctcgtcga
    10801 gcgccagctc gtgcggatcg ccgtcgtcgg gcaggcgtcc gtcgaccacg aagggccggt
    10861 aggagcgagc tccgtcgacg gcgatcggga tgaacgggtt gacgtcgccc gacgggaacg
    10921 gtccgccacc cgggccttcg gcggtgagca gcccgtacgc tcccgtggac cagccggcga
    10981 cgtgcgggag ggcggtgacg gcgccgacgt cgaaggagcc gccgtccggg gtgtacacct
    11041 gggcgtcggg aggccggttg tacgcgagga accggtcgag cgacgacgac gctcgtcgtc
    11101 cgccggcgaa ggcgccgccg gccgccccca ccacgagcgc cacgaggagc gtgaggagca
    11161 ccgtcgccgg gatgttccgt cgcaaccacg cacgagcaag tgacccggac ggccccatca
    11221 cgccacgcga cccttcgtca tcgaactaga gcaggaccgg cgccggcgtc gccgacggct
    11281 cggcctcggg gacctcgacg cgtccgtcgc gcatgcgcac gatgcgatcg gcggcggccg
    11341 cgacgtcgtc gtcgtgggtg accatcaaga tggtctgacc ctcggcgtgc aggcgccgga
    11401 acagctcgag gatctcctgg ccgccgtcgg agtccagcgc cccggtcggc tcgtcggcca
    11461 ggacgagggt cggctcgttg gcgagggccc gggcgatggc caggcgctgg cgctgcccgc
    11521 cggagagcac gcccgggagc tgccgggcct tgtcggagag gccgagcagg tcgagcaggt
    11581 cccgcgcccg ggtctcggcc ggcttgcgct tgaggccggc gatcatggcc gggagggcga
    11641 cgttctccag caccgtcatg ccctcgagga ggttgaagaa ctggaagacg atgccgatgt
    11701 gacggcgccg catccgggcc aggtcgtcct cgtccatggc atggatctgc tcgccgtcca
    11761 gctcgaccgt cccctcgtcg acggcatcga ggccggcgac caggttgagc atcgtggact
    11821 tgccgcaacc agacggaccc atgacggcca cgaactcggc ccgggccatc tcgaagtcca
    11881 ggccgcggag ggcccgcacg ggcgcctggt cggcttcgaa ggtcttgcgc accccgccga
    11941 tctcgagcac cgggtcgccg atgcctccca cgtgcacggt catgctccaa ctcctgttcc
    12001 ggcccgccgg acggcgggca ccttgtacgc ctggaccggg gtgtcccgac ccttcacgac
    12061 tgccggttcg agcgcgtcgg cggcgaccgc cgtccggagg gtgcgccagg tcgcgtccgt
    12121 gagcacggtc tcccccgcct cggcccactg ctggagccgc tgggtgatgt tcaccgcgtc
    12181 gccgaccacc gtgtactcca gccgctcctc gctcccgagc agcgcggcgg cgacctcgcc
    12241 ggtggagacg ccgatgccga gcccgaacgg tgggaggccc tgctcctccc accgctcgtt
    12301 gatctcgtcc tgcgcgtcgt gcatggcccg cgccgcggcg acggcgcggt cggggtggtc
    12361 ggggcacggc agcggcgcgc cgaacaccgc catgaccgca tccccgacga actgcatgac
    12421 cgtcccgtcg ttgccgagga tggcgcggtt catcgcggcg cggtgctcgt tgagctggcc
    12481 ggcgagtgcg gtcggatcca ctcgctcggc gatcgtggag tagccccgga tgtcggccat
    12541 cagcacggtc acgaccatcc gctcggactc cccgatcacc atgccgccgg cccggacctg
    12601 ctcggcgacg ccaccgggca gcaggcggga gagggactcg ccctgctcct cccggtcgac
    12661 gatggcctgg tggagcatgc gcagccgccg gagcgccgga tcggagccct gcctcgcccc
    12721 tcgggccagg cgcaggaaca tcgcgtcgac cgcctcggcc acgatcgccg gcgtcgtctg
    12781 ctgcatggcg gcgatcgcct tgatcggctt gccctcggcg accatctgca ggagggcctc
    12841 gtcggcgggg cgcagcgccc cgtccgacga gaccggggtg accagtgcct ggacgatctc
    12901 cgggtcgagc atcgacccgc ccgtcgcaac ggtcctgatc gccgccgcca ggtggtcacc
    12961 ctccgccacc cgatccttca ggaggtaggc gtagccggcc gctccgtcgt tgagcagcga
    13021 gaccgcgtac tccgggtcgt cgaactggga cagcaccacg acgccggtgc cggggtgccg
    13081 cttgcggatc agcttcgccg cctcgatgcc ctcctgctgg aagctcggcg gcatccggat
    13141 gtcggtgacg aggacctggg ggttccgctc ctcggcaccg gccaccaggg agtcgaagtc
    13201 gtcggcgacg ccgacgacct cgagatccgg ctcaagcccg agcagggcac ggacgccctc
    13261 gcgcacgatg aggttgtcat cggcgaggaa caccgagatg gcgctccgca cgccaccggc
    13321 ttcggttgtc tcgatcaccg tccgcctcca ccagggagag ctccccagct cgtcgacacc
    13381 cctcggcaga tcgtaaccac gtccgtgccc cctgtctcgc cctggcggtg cgcctgctca
    13441 gccgacctgg aaggtctgct tcatgccgag ctcgtagtgg gccttgccct ccgggccggg
    13501 gatgaagcag acgacggcgt aggtgccggg cgtgagctcc tcggcgttca ggtacacgcc
    13561 accctcaccg gggggtgcga aggtgaagcc gacctcctgg gcctcctcct ccgtcggctc
    13621 ctgctccgat gccaggagct cgtccatgtc gacgccctcg cccagcttga acacggccat
    13681 ctcgtgggct gcgtcgccct cgttggtgaa ctcgaacgac acggggcccg cgggcaccgt
    13741 gtccgggagc ccttcgaagg cgaagtcgat gcccgtcacc gggatgacct cggcgccgtc
    13801 gagcggctcg gtggcagcct cctcctcctc gtcctccgaa gcctcgaagc cgcagacctc
    13861 ggcatcgtgc gcctccatgg cgccgaaggc ctcctcgatg gccgggtcgc tgaagaccgc
    13921 gcccatgtcg ccgtcggccg cgatgaacgc gtcgaccacc atgtcggtct cctcaccgat
    13981 gctctccggg cggatctcct tcagctcgag gagctgctcc tcggtcggcg gaccgtcctg
    14041 gctgtcgagc tcctcgatcg ctgcgcagta ctcctcgacg tcggtgtcgg ccgcctcgcc
    14101 ctcggcgagg gtcgtggccg ggtcgtccgc ggtgctggtc tcagcgtcgt cgtcgtcgcc
    14161 gcacgcagtg gcgaggagac cgaaggcgca gacggcagcg agtgccttca cgcggcggga
    14221 tcgggtggtc gtgggcatgg gtcctccagg acggttctcg tggctttgcg ccatctggcc
    14281 acggaacggc ccgccgaccc agcccgcagg cacgagatcc cggggtggtg cctgcctccc
    14341 cccggccgac gggtcagcgc cctggccgcc gccgttcgcc ggcgcgacgg tgcctccatg
    14401 cgcatcgacg gttcggtcac ctcggtcagc tggatcccct ccgaggcggt gaaggggatc
    14461 atgaagctcc cgttctccat gggggtcgcc cactacgacg accccccgcc cgagcgcctg
    14521 ccgggcgcgt cggtgccgag cgcgctcgac gagctgcgcc gtgccgaccg gttccgcttc
    14581 gccaaccacc tgaccgcctg ggtcgaggtc gacgccggcc gcatcgtgcg ctgggggcag
    14641 gggggcgagc cgatgatcgg ctcgacgacc atgcgcgccg gtcgttccct cacggtcgcc
    14701 gccgtggcgt tcgaacccat caggccggaa cccgaggtgg gcgagggctg ggtccggttc
    14761 cgccagtcgg ccggtgggcg caccggtgtc cccgctccgc gtcacgtgag ccgcccgccg
    14821 ttcgtgcagg tgtcggcacc gtcggcgtgg acgaccctcg agctgaccct gcacgccgac
    14881 gggcgccagg agctcggtct ggccggcgcc agcccgttcc cccgccactg gctgtacgac
    14941 ggtgccggga tgctcgtccg caagagcggc atgatcgact tcaaggactg gtaccggcgg
    15001 gcgttcggca cgcacagccc gtggggcgac gaggactcgc ctgccctcgc caccgaggtc
    15061 gagaccgcgc tggagcgcca gctctcgacc acgatcatgc gcggcggcac gcggccccgg
    15121 atccgcaagg tcgtcgccgg tgatgcgctg gtgcagcagg gcgacgaggg cgaggagctg
    15181 ttcctgctgc tcgacggcgt cctcgcggtc gaggtcgacg gcgagcgcct cgccgagctc
    15241 ggtccgggtg ccgtggtggg cgagcgggcg ctgctcgagg gcgacctgcg cacgtcgacc
    15301 ctgaccgccc tgaccccctg caaggtggcc gtcgcccgcg ccgatgagct cgaaccggcg
    15361 gccctcgggg cgctgcgtga gacacaccgg cgcgaggacg cccgctgagc ggatgcgcct
    15421 ccacctcctc ggcgtccgtg gctcgacccc gacaccggga ccggcgttcg cccgccacgg
    15481 aggccacacg tcctgcgtcg cggtcgaggc cgaggacggc agtgtcccga tcgtgctcga
    15541 cgccggcacc ggcctgcgct cgctcggtcg ggtcctcgac ggacgagcct tcgtcggcac
    15601 cctgctgctc gggcatctgc actgggacca cacgcagggg ctgccgttca gcccggtggt
    15661 cgaccatccc gacgcacggg tccgggtcct ggcgccgacg gcatgcgggc agtccgtcga
    15721 ccagctgctg gcgcaggcca tgtcaccgcc caacttcccg atccgcccgg acgagctgcg
    15781 gggggactgg tcctggggcc cgctcgtcga ggggcaccgt tcgctcgagg gcttcgaggt
    15841 cctggcccgg gagatccccc acaagggagg ccggacgttc ggcttccggg tgagcgacgg
    15901 gacccgtgcc ttcgcctacc tctccgacca catgccccaa gcgctggggc cgggtcccga
    15961 cggactgggt gcccgacacg acgccgccct ggccctggcc gacggcgtcg acgtgctgat
    16021 ccacgacgcc cagtacacgg ccgaggagct cccgacccgc ggcgccttcg ggcactcggc
    16081 gaccgagtac gccgtggcgc tcgggcagga ggccggcgcc ggccgggtcg tgctgttcca
    16141 ccacgacccg caacgcaccg acgacgagat cgacgcgatc gtcgctcgct tcaccgatgc
    16201 gccgatgccc gtcgtggccg ccgtggaggg ggcgacgatc agctgctgac cggccgggat
    16261 ctggcagcgg ccacggaacg ccgacacgat ccacgccgga cgtgcgacag ttcgtccatg
    16321 cctacgcacg acgacgtgga cctcggcctg ggctcgatga tcgagcgccg gacccacctg
    16381 accccgaacc tccccgccct caccttcgag ggcaccacgt ggacctacgc cgagatgctg
    16441 gaccgcatcg accgcttcgc caccgtgctc gccgacggcg gcatctcccg cggcgaccgg
    16501 gtcgcgtacc tcgggctcaa ccacgccaac ttcttcatca cgctgttcgc caccgctcgc
    16561 ctcggcgcga cgttcgtgcc gctcaacttc cgcctcaccg gtcccgagct cagcttcatc
    16621 gtcggtgacg ccgacgtgca cacggtcgtg gccgacgaca tgcaccgccc cgtcatcgac
    16681 agcatccgcg acgacatccc gtgccaggtc cacctgtcgt cggagaccgc cgccgacggc
    16741 tggccggcga tggacgacct ggccgccggc gccgcaccgc tcaccgatcc cgtgcgcgcg
    16801 gcgcagggcg acgtcgccgt gatcatgtac acctcgggca cgaccggccg gcccaagggt
    16861 gcgatgctca cccacgccaa catcgcgtgg aacaacgtga acgcgctgca cgccatcgac
    16921 ctcggtgcgg acgaggtcgc gctcgtcgtg gcgccgctgt tccacatcgg cgggctcaac
    16981 gtcaccacgc tgctcgtgtg gcagaagggc gggcacatcg tgctgcaccg cgccttcgac
    17041 ccggctgccg cgctgcgggc catcgaggag cacggcatca ccaacatgtt cggggtgccg
    17101 gccatgtacc tgttcatgag ccaggtgccc gggttcgacc aggccgacct gtcgagcgtg
    17161 cgggtgttcg tggtcgg
//
LOCUS       DATISU010000034         4732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_513931, whole genome shotgun sequence.
ACCESSION   DATISU010000034 DATISU010000000
VERSION     DATISU010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513931"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1052
                     /locus_tag="VLR27_01695"
     CDS             198..1052
                     /locus_tag="VLR27_01695"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HSP02184.1"
                     /translation="MLPLKDVNPTSRRPVVTLLLILACVGAWFLWQEEPQREPIEDLQ
                     FNIENTAIPCELTRGRPLTVPEVEATFGTLGGDVESCGIAGDDAPRFVPDKSVWLAAL
                     ASMFLHGGLMHIGGNMLYLWIFGNNIEDHLGAVRYLLFYVVGGIAALATHVALQPDST
                     IPLVGASGAVAAVMGAYLVWFPNAPVRTIVLLGFPILATIKAKWLLAVWFGLQFLTSP
                     NSGVAWAAHVGGFVFGVAIGLLVRSIRAAQKAAWRSRYQDQMSPHHWDSTGGVGPVHE
                     YQRWRPGR"
     gene            complement(1056..2804)
                     /locus_tag="VLR27_01700"
     CDS             complement(1056..2804)
                     /locus_tag="VLR27_01700"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HSP02185.1"
                     /translation="MTSGEHAPAEPPANRLGDLDRVFARAGTMAPILQDLDWASTDLG
                     HPDTWPQSLRSAASICLGAKYPIAIYWGDELTLLYNEAWSMIPGDKHPWALGRPAREV
                     WPDIWHIVGPEFDRALRGEGMWVADRLLPMERRGYLEETYFNYNLSPITAEDGRIEGV
                     FNAGLETTPRVVAARHQRVLIDLIAETASATTVGEAFVGATEALAEHREVAPFAVLYR
                     VDDGVAHLSGSTGVAEGGEGTGASVTLGVDADPWHIQEAIESRRAVVVEDIGRFADVH
                     AYEGWPETAHTAVTLPVVRSRRRRRRVEGVLVVGVPPGLRIDPQHREFHAALADRFAL
                     SVLDARREEEERRMFEVEHRIAATLQQSLIPALPDLDDVDLAGCYLPGTEGLEVGGDW
                     YDAIPLPDGSVTMVIGDVVGKGVAAAAQMGQLRNALRAYVLEGFSPGEVLAKLNHLTL
                     SLSGTTFATLLCLRYEPDSHMVRWCRAGHLPALVRHADGRVSLLDEDGCPPIGVFEGV
                     AFGESERRLEPEDIVVLYTDGLIEKPGEDIDVSIDGLARRIAGLPVDAGFVDAIVAPL
                     ADSERRDDVAVVVLST"
     gene            complement(2846..3589)
                     /locus_tag="VLR27_01705"
     CDS             complement(2846..3589)
                     /locus_tag="VLR27_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSP02186.1"
                     /translation="MPWVLDLDGVIRLGEQPIDGAAEAVAMLRAAGEEVVFATNNAYR
                     SIRDQEAALDAIGIPAAGAVVGAAQAGASLLEPGERVLVVGGPGLIEEVEARGCEIVE
                     DGPCDAVISGLDRQFHYDDLRRAGHAIRAGARWVLTNPDPTFPTPHGLEPGAGALGAA
                     IRAVGGIDPLIGGKPEGAMVGLIRDRLGGDGIVVGDRADTDGLFATALGYRFALVFSG
                     VTTKADLPVEPEPWMVADDLLEVVRKTLD"
     gene            3633..>4732
                     /locus_tag="VLR27_01710"
     CDS             3633..>4732
                     /locus_tag="VLR27_01710"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HSP02187.1"
                     /translation="MPRFEPFRGLRYDRERVSLADVTAPPYDVIDDRQRAELARNDRN
                     AVRIDLPIDEDGEDRYDVARRLLHEWQDEGTFVTDEAPTFTIYRMTFTDEAGVTRQTT
                     GVIGALELWPPGAGEILPHEHTTKKAKSDRLDMLRSTRYNLSAIWGLSLADGLTDLLP
                     TDVEPDAEWTDPDGVTHTTWTSRDADRNGRIATLVLDHPIVIADGHHRYETSLQYRDE
                     RRIVDGPGTAADLTMCFVVELVEDELTVQPIHRLITGLPDGASLPVLLSEHFDVEPTD
                     PFGPDAVARLVELGGLGLVDDTGTFVLRPKDGAFDASVELDTVRLEQARAALPAHDLS
                     YQHGVANVTEAVAEGRAQAAVLMRPATVAQIQA"
BASE COUNT          672 a         1767 c         1629 g          664 t
ORIGIN      
        1 ctcggcctgc ttggacttga cctcgccctg cttgacgagg tccttcacga tcgcctcggc
       61 gcgggactgg gtcatctgcg tgaacgccat ccccgcatcg aggtaccgct tgatcagggg
      121 gttcttggcc atccgggcag gctacgacag cacggcgggg ggcgaaagtg ctgggtggcg
      181 cctcctacgc tcgggcggtg ctccccctca aggacgtgaa ccccacgagc cggcgtcccg
      241 tggtcacgct gctgttgatc ctcgcctgcg tcggtgcgtg gttcctgtgg caggaggagc
      301 cccagcggga gccgatcgaa gacctccagt tcaacatcga gaacacggcg atcccctgcg
      361 agctcacccg gggccggccg ctcaccgtgc ccgaggtgga ggcgaccttc ggcaccctcg
      421 gcggcgacgt cgagtcgtgc gggatcgccg gggacgatgc gccccgcttc gtgccggaca
      481 agtccgtgtg gctcgctgcc ctggcatcga tgttcctgca cggcggcctg atgcacatcg
      541 gcggcaacat gctctacctg tggatcttcg ggaacaacat cgaagaccac ctgggcgccg
      601 tccgctacct gctcttctac gtcgtcggcg ggatcgccgc gctcgccacc cacgtcgccc
      661 tccaaccgga cagcaccatc ccgctggtcg gcgcgtccgg cgccgtggct gcggtcatgg
      721 gcgcgtacct ggtctggttc ccgaacgcgc cggtgcgcac catcgtgctg ctggggttcc
      781 cgatcctggc caccatcaag gccaagtggc tgctggcggt gtggttcggc ctgcagttcc
      841 tgaccagccc gaactccggc gtggcctggg cggcccacgt gggcggcttc gtgttcgggg
      901 tcgccatcgg gctgttggtc cggtcgatcc gggccgcgca gaaggcggcc tggcggtccc
      961 gctaccagga ccagatgtcg ccgcaccact gggactccac cggcggcgtc ggaccggtcc
     1021 acgagtatca gcgctggcgt cccgggcgct gaggttcagg tgctgaggac gaccaccgcg
     1081 acgtcgtctc ggcgctcgga atcggcgagg ggcgccacga tcgcgtcgac gaagccggca
     1141 tcgaccggca gcccggcgat ccggcgggcc aggccgtcga tgctgacgtc gatgtcctcg
     1201 ccgggcttct cgatgaggcc gtcggtgtag agcaccacga tgtcctcggg ttccagccgc
     1261 cgttcggact ccccgaaggc cacgccctcg aacaccccga tcggcgggca gccgtcctcg
     1321 tcgagcaggg acacccgacc gtcggcgtgg cgcaccaggg ccggcaggtg gccggcgcgg
     1381 caccaccgca ccatgtggct gtcgggctcg tagcgcaggc acagcaacgt cgcgaacgtg
     1441 gtaccggaca gcgacagcgt gaggtggttg agcttggcga gcacctcacc gggcgagaag
     1501 ccctcgagga cataggcgcg cagcgcgttg cggagctggc ccatctgggc ggcggccgcc
     1561 acgcccttgc cgacgacgtc gccgatgacc atggtgacgc tgccgtccgg cagcgggatg
     1621 gcgtcgtacc agtccccgcc gacctcgagg ccctcggtgc cgggcaggta gcacccggcg
     1681 aggtcgacgt cgtccaggtc gggcagcgcc gggatcaggc tctgctgcag ggtcgcggcg
     1741 atgcggtgct cgacctcgaa catccgccgc tcctcctcct ctcgacgggc gtcgagcacg
     1801 gagagggcga accggtcggc cagggcggcg tgaaactcgc ggtgctgggg atcgatgcgc
     1861 agcccgggcg gcacgcccac gaccagcacc ccctcgacgc ggcgtcgccg gcgtcgggac
     1921 cgcacgacgg gcagggtcac ggcggtgtgg gcggtctcgg gccacccctc gtaggcgtgc
     1981 acgtcggcga agcgaccgat gtcctcgacc accacggccc ggcgggactc gatcgcctcc
     2041 tggatgtgcc acgggtcggc gtcgaccccg agggtcacgg acgcgccggt gccctcgccg
     2101 ccctcggcga ccccggtgga gccggagagg tgcgcgaccc cgtcgtcgac gcggtacagc
     2161 acggcgaagg gtgcgacctc acggtgctcg gccagggctt cggtggcacc gacgaaggcc
     2221 tcacccaccg tcgtcgccga cgcggtctcg gcgatcaggt cgatcagcac ccgctggtgc
     2281 cgggccgcca cgacccgggg cgttgtctcg agcccggcgt tgaacacgcc ctcgatgcga
     2341 ccgtcctcgg cggtgatcgg gctcaggttg tagttgaagt acgtctcctc gaggtacccg
     2401 cgccgctcca tgggcaggag ccggtccgcc acccacatcc cctccccccg gagggcacgg
     2461 tcgaactccg gaccgacgat gtgccagatg tcgggccaca cctcccgagc gggccgcccg
     2521 agcgcccacg gatgcttgtc gccggggatc atgctccagg cctcgttgta gagcagcgtc
     2581 agctcgtcgc cccagtagat ggcgatcggg tacttggcgc cgaggcagat gctggccgcg
     2641 gagcgcaggc tctggggcca cgtgtccggg tgtcccaggt cggtggaagc ccagtcgagg
     2701 tcctgcagga tcggcgccat cgtgccggcg cgcgcgaaga cgcggtccag gtctcccagc
     2761 cggttcgcgg gcggctccgc aggcgcgtgc tcgccggatg tcacgctcaa gggccgacac
     2821 cctacgcggc tgcacgcacg gacggtcagt ccagggtctt ccgcacgacc tcgagcaggt
     2881 cgtcggcgac catccatggt tccggctcca ccggcaggtc cgccttcgtg gtcaccccgg
     2941 agaagaccag ggcgaagcgg tacccgaggg cagtggcgaa gaggccatcg gtgtcggccc
     3001 ggtccccgac cacgatcccg tcgcccccga gccgatcgcg gatcagcccg accatggcgc
     3061 cttcgggctt cccaccgatc aacgggtcga tccccccgac cgcgcggatc gctgcgccca
     3121 gggcgccggc gccgggttcg aggccgtggg gcgtggggaa cgtggggtcg gggttggtga
     3181 gcacccagcg ggcacccgcc cggatggcgt ggccggcgcg acgcaggtcg tcgtagtgga
     3241 actggcggtc cagccccgag atcaccgcgt cgcagggacc gtcctcgacg atctcgcacc
     3301 cgcgggcctc gacctcctcg atcagcccgg ggccgccgac caccaggacc cgctcgccgg
     3361 gctcgagcag gctcgcgccg gcctgtgccg caccgacgac ggcgcccgcg gcggggatgc
     3421 cgatcgcgtc cagcgcggcc tcctggtccc ggatcgaccg gtaggcgttg ttcgtggcga
     3481 acaccacctc ctcgccggcg gcgcggagca tggccaccgc ttcggctgcg ccgtcgatcg
     3541 gctgctcacc gagccggatc acaccgtcga ggtcgagcac ccagggcatc gcagcatcgt
     3601 ggcacccgga cccggtctgc aacgatgtcc gggtgccccg cttcgagccc ttccgaggtc
     3661 tgcgctacga ccgcgagcga gtctccctcg ccgacgtcac cgcacccccc tacgacgtca
     3721 tcgacgaccg ccagcgggcc gagctggccc gcaacgaccg caacgccgtg cgcatcgacc
     3781 tccccatcga cgaggacggc gaggaccgct acgacgtggc ccggcggctg ctgcacgagt
     3841 ggcaggacga gggcaccttc gtcaccgacg aggcccccac gttcacgatc taccggatga
     3901 cgttcaccga cgaggccggc gtgacccgcc agaccaccgg cgtcatcggc gccctcgagc
     3961 tgtggccccc cggggcgggc gagatcctcc cccacgagca cacgaccaag aaggccaagt
     4021 cggatcgcct cgacatgctg cggtccaccc ggtacaacct ctcggccatc tggggcctgt
     4081 cgctcgccga cggcctgacc gacctgctcc ccaccgacgt cgaacccgat gccgagtgga
     4141 ccgaccccga cggcgtcacc cacaccacct ggaccagccg ggacgccgac cgcaacgggc
     4201 gcatcgccac cctggtgctc gaccacccca tcgtcatcgc cgacggccac caccgctacg
     4261 agacctcgct gcagtaccgc gacgagcgtc gcatcgtgga cggcccgggc accgcagccg
     4321 acctcaccat gtgcttcgtg gtcgagctgg tcgaggacga gctcaccgtg cagcccatcc
     4381 atcgcctcat caccgggctg cctgacgggg cgtcgctgcc ggtcctgctc agcgagcact
     4441 tcgacgtcga gcccaccgac ccgttcggcc ccgacgcggt cgcccgcctg gtcgagctcg
     4501 gcgggctcgg cctcgtcgac gacaccggca cgttcgtgct ccgccccaag gacggcgcgt
     4561 tcgacgcctc ggtggagctc gacacggtgc gcctcgagca ggcccgcgcc gccctgccgg
     4621 cccacgacct cagctaccag cacggcgtgg ccaacgtgac cgaggccgtg gccgagggcc
     4681 gggcccaggc cgccgtcctc atgcggccgg ccaccgtggc ccagatccag gc
//
LOCUS       DATISU010000035        12714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_793243, whole genome shotgun sequence.
ACCESSION   DATISU010000035 DATISU010000000
VERSION     DATISU010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 12714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12714
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793243"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..798)
                     /locus_tag="VLR27_01715"
     CDS             complement(196..798)
                     /locus_tag="VLR27_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP02188.1"
                     /translation="MPEQIVTTDGRRARRERNRVAVVDAVFELINERHSPPGVEEIAV
                     RAGVSVSSVFRYFENLDDLLEQTVARYFERFSALFTVPPAPGGTLDDRIAALVSARLD
                     LYEAIGPIARIARLRATEQPRLAAVLADTRRTFEGQVRSHFAPDLAGRARDDVDDRVA
                     LVDCLTAFESWDLLTGTHGRSRTLVARAWVTGLRDLLAAA"
     gene            878..3163
                     /locus_tag="VLR27_01720"
     CDS             878..3163
                     /locus_tag="VLR27_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HSP02189.1"
                     /translation="MGETGEVQVRADEFRGRIGPTIAESEPWFDEQPHPGEGAPNVVV
                     VLLDDTGYAQLGCYGSDIDTPNIDALAAGGIQFTNFHVTPLCSPTRASLLTGRAPHAV
                     GMRSVSNFRTGFPNQRGHISNHACTVAEVLHDQGYATFCVGKWHLAPMEQCSAAGPFD
                     QWPLARGFDRFYGFLEGETDQFHPELVADNHPVDPPGGPEDGYHLSEDLVDQTLRMIA
                     DSTGVRPDRPFFTYLAFGATHAPHQAPPDFLEKYRGRFDEGWDVVRERWFHRQLELGV
                     IPPDTRLAPRNPGVEPWDELPDDQRRLAARLQEAFAAFLDHTDVQIGRFVDGLRRLGQ
                     LENTIVVVLADNGASQEGGPYGVLHEMKFFNGILEAPAEAVAHIDDIGGPRSHTNYPW
                     GWAQCGNAPFRWYKQNTHEGGVHVPMVVHWPAGIDAAQHGTKRDQFAFVADIVPTLYD
                     VVGIDPPGVRNGLEQLPVTGHSFAAALHDPAAPATNTVQYFENAGSRAIIADGWKAVT
                     KHVAGADYDTEPWELHHLAADPSETEDLAEREPQRLQAMVRLWWAEAERHGVLPLDDR
                     LVELFGARFRERSPHPPDRRYVYRPPMSPLPGQAGAAIAGRSFDLTAAVTHDPAAEGV
                     LFATGTENAGLSLFVQGGRLVLDYNAFGDHTVVESDVAIPSGDVHLGVRLRRLDGFAG
                     TATLTVGGADAGHAELSLLMRMMSSIGPSIGADHGSAVSDRYAAPFPYGGTLHEIVIQ
                     ASPDRYGDTAATSARAEMSRQ"
     gene            3191..4102
                     /locus_tag="VLR27_01725"
     CDS             3191..4102
                     /locus_tag="VLR27_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HSP02190.1"
                     /translation="MQTLRTPDGAFADLPDFPFEPHYVEVDDGEGATLRIHHLDEGPA
                     DAAPVLLMHGEPTWSYLYRHVIPGLVEAGHRVIAPDLVGMGRSDKPTEQADHTYARHV
                     EWMRQALFDRLDLRDITFFGQDWGSLVGLRLVALDPDRYARVAIGNGGLPTGHGPASE
                     AFLAWQRFSQETEHFPVGRIIDGGCTTSLSPEVIAAYDAPYPDDSYKAAVRVFPSLVP
                     TSSDDPATPDQEAAWRVLERFEKPFLLCFSDSDPITKGGDAPFLAKVPGAQGQAHTTI
                     EGGGHFLQEDRGPELARVLAGFIEATS"
     gene            4099..4983
                     /locus_tag="VLR27_01730"
     CDS             4099..4983
                     /locus_tag="VLR27_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02191.1"
                     /translation="MILDDAGWRALTADAALASHRLIGWIYWDPTAVANYAALGVPDG
                     AGYYFATRAASIAQAGDDVVTATFGSIHPAFISACLDLCRQHTTFEAAALARDAAVEP
                     GLRALAPDLCDGLTELGPHLWAAADELVLTGRPLFASLLRRRREDQPLLSAWLAVNCI
                     REWRGDTHWAIHAVEGIDGTMAGLLDGAWRGHDDDWLPRSRGADDALLDAALAELEAR
                     GLATDGRVNATGVAHRQQLEDRLDDLCTPGWQALGEPLTRRLVELIEPYGSTFVERID
                     QTAGPRWMPAARDRTVRA"
     gene            4980..5435
                     /locus_tag="VLR27_01735"
     CDS             4980..5435
                     /locus_tag="VLR27_01735"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSP02192.1"
                     /translation="MTATSSRTSTVATSPEAVWEVLADFGALARWVPEVDHSCLLRSG
                     PAGVGTTRRVQVGRTTVLEAITAWSPPEHLGYAITGLPPALQTVRNDWRLRPVADGTE
                     VTVTTSVDAGPRPPQRLVAHLVARRLSTTSGQMLAGLTATLTSGDHARA"
     gene            5428..8013
                     /locus_tag="VLR27_01740"
     CDS             5428..8013
                     /locus_tag="VLR27_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSP02193.1"
                     /translation="MPETRPDVVVLLTDEERAAPPYETEALRAWRRRALPGARWFEEH
                     GVSFARHHTGSLACVPSRPTLLTGQYPDLHGVSQTDGLGKMADDSRMRWLPPEEVPTV
                     GSWFRAAGYDTHYDGKWHVSHADLTDPTGAPLATNDAEGNVDPDAVQAYLDADPLGPY
                     GFSGWVGPEPHGAPMANSGLVRDPLIADRVVAWLEDRYARRRAGDPEALRPFLLVASF
                     VNPHDIVLFPAWVRGRWPLEPSPVDPPSVPPSPTDDEDLRDKPAAQVAYRAAYPSGYG
                     PAPAVARIYRRGAQRYRDLYHRLHAEVDGPLDRVRRAVTEQGSEHAVLVRTSDHGELL
                     GAHGGLHQKWFNLYDEATRVPFVVARVGERATEARTVDAPTSHVDLVPTLLAAAGIDV
                     DATARELASTFSEVHPLPGRDLLPVVDGAEADPDRVVYLQTRDNMLEGDTGASGVARR
                     LGRTERPPAPLRIRVPAHVASSFEGVVARVRDEVAAGGAGHRWKLVRTFDDPSTWTEP
                     GVRHLADTGPGGPTYRTEPLEDQWELYDLDADPVEIRNRWRHPDATAVLEHLRDVLKA
                     EASRSIPERNRPLTPLRWHPAGHPAKTPPRPALALRRLVQRLGMHPEDTDADHSVDRS
                     GRRALVVCTNHGVLDVGKPTGVFASEMTVPYYAFRDAGMVVDIASPAGGTVPIDPQSL
                     TPVLRCGSDDRFLADAELRAKVTDSLSIADLDIAGYDLVYLAGGWGAAFDFGTSQALG
                     DQLSRAEELGLPIGGVCHGPLALRLATAADGRPLVEGRRISCVTDKQVHELGIASTPM
                     HPETELRALGARFEARHRFRDPFANHWVVDGNLVTGQNQNAGPMVARELLALVPADPR
                     SEPRT"
     gene            8010..8846
                     /locus_tag="VLR27_01745"
     CDS             8010..8846
                     /locus_tag="VLR27_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02194.1"
                     /translation="MTDTRTPGYGQVDRDYGIRLATTPPGADGPIWMVNLMSYRGRAV
                     YDGAEADVTGREADDRYAPVDVLADIGAQVAFVADVEDQLLGDSPRWDRIGVVRYPTR
                     RSFIEMQSRPDFKAKHVHKEAGMAETIVAGCTPIASPQDRPDAPTPVDWSSVPHPPTD
                     ADGPVVVLHLIRFHPGGADTDMVRYQDDAARVAVPHGVRIAGWFGVEGTIVGDGRRWD
                     QVRFNRFPSKAAFLAVATDPGRLESQAAHREAAIADTYALILRPTIDRLDASVAEVGG
                     IS"
     gene            complement(8839..9570)
                     /locus_tag="VLR27_01750"
     CDS             complement(8839..9570)
                     /locus_tag="VLR27_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSP02195.1"
                     /translation="MIRIAAVGDIHLGADNVDAFRESVRTAEDDADLLLLAGDLSRHG
                     EVEEAELVCRSVSDLDMPVVAVLGNHDHHAGCADAFADQLTSSGITVLEGSSTVVDVD
                     GTTVGIAGAKGFGGGFAGACASDFGEPEMKAFVRHTKGIADQLCHTLAALDTDLRIAL
                     LHYSPVEDTLRGERLEIFPFLGSYLLAEAIDGAGADLALHGHAHAGVEHGMTAGGTRV
                     RNVAMPVIRSSYRVYELDPALSGVS"
     gene            complement(9567..9833)
                     /locus_tag="VLR27_01755"
     CDS             complement(9567..9833)
                     /locus_tag="VLR27_01755"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSP02196.1"
                     /translation="MSERPMDDSPEYLAERVHQAVTTDPRTTEQGVQVRVTEVEVFLF
                     GEVASAERRAAVAEVAAEVVPDRRIHNDVTVVPLDGEHTPERIR"
     gene            complement(9830..10447)
                     /locus_tag="VLR27_01760"
     CDS             complement(9830..10447)
                     /locus_tag="VLR27_01760"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HSP02197.1"
                     /translation="MDGHVPDEQHLLDTLGDVVRCLEASDTDHLLMGGIGSFAMARPR
                     ETHDIDVFLRPGDVEKALDLLEHDGFETTIHDPAWLAKAFKRRVLVDLIFRSSGEIYL
                     DDEMLERSERRDYKGVSARVISAEDLLVIKALATGETTAHHWYDALALIAKCDLDWDY
                     LVRRANQAGPRRVLSLLIYAESCDLAVPTTAITSMHDTIHGGVVA"
     gene            complement(10522..10758)
                     /locus_tag="VLR27_01765"
     CDS             complement(10522..10758)
                     /locus_tag="VLR27_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02198.1"
                     /translation="MVAIAGAQLTRRAEVLALAVTALVALAVVIIPAAYLAALPLACG
                     AMALSAPRGRRLPASIAVWASVSLAVVVGGVPIA"
     gene            complement(10908..11789)
                     /locus_tag="VLR27_01770"
     CDS             complement(10908..11789)
                     /locus_tag="VLR27_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02199.1"
                     /translation="MRIHKLSSTDAFVAFDLDDAPSVGVTRLARKVLTDGAELLARST
                     TYAFASFGIQMGGGSAGINAEGDDRDPAVAAFVEEMRSAVAEGRWATDPALGLAEADL
                     AQLRIDDRRPSELWTDGLAETLTAQGAVAAAGAARDGGLTGATCAFVGKGPVVDAARA
                     AVEAAGGTVAEGDSPATQADVLFVAGKTGVLDHEVVPDITAGVVVPLTPVPVTARAHA
                     ELTKAGTVHVPDFLSTAAPLLHAHGDDVTDPVAAVADKVAGLASHGTGMWLAAVEEAE
                     AFLRTWRDELPFGRPLA"
     gene            11868..>12714
                     /locus_tag="VLR27_01775"
     CDS             11868..>12714
                     /locus_tag="VLR27_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSP02200.1"
                     /translation="MPASTEEPLPSEPADQLFPEPTDRIGPGSGPFGAPLLDGLNPAQ
                     EEAVTHLGGPLLVLAGAGSGKTRVLTRRIAWLIQEQGVSPFEILAITFTNKAADEMKA
                     RVGTLVGSVAQKMWVSTFHSACVRILRRDAGAIGFPSSFTIYDQADAVRLTGYVLRDL
                     NLDTKKMPPRSVHAQISAAKNDYVLVDEYADRAGSFTERKLADVYREYQARLLRAGAM
                     DFDDILLNAVELFRRHPDVLAHYRQRFTHVLVDEYQDTNKVQNELVVQLAKEHRNVTV
                     VGDGDQ"
BASE COUNT         1751 a         4810 c         4474 g         1679 t
ORIGIN      
        1 gcggcccggg cccagctccc cgccaccggc ggcgacgcgg ccagcactgc gggcctggct
       61 gcggccatgg ccggtggcgc actcgccatc gtggctctgc gccgacgcct gggctgacgc
      121 ctcgaagggc tgacgcctcg acgactcgct gcgggcggtt cctcctcgga ggggccgccc
      181 gcagcgcgtc cgggctcagg ccgcagcgag caggtcgcgc agcccggtga cccaggcccg
      241 cgcgacgagc gtccggctcc ggccgtgcgt gccggtgagc aggtcccagg actcgaaggc
      301 ggtgaggcag tcgacgaggg cgacccggtc gtcgacgtcg tcgcgggcgc gcccggccag
      361 gtcgggggcg aagtgcgagc gcacctgccc ctcgaaggtc cgtcgcgtgt cggccaggac
      421 cgcagccaga cggggttgct cggtggcgcg cagtcgagcg atccgggcga tcggaccgat
      481 cgcctcgtag aggtcgaggc gggcggagac cagggcggcg atgcggtcgt cgagggtgcc
      541 cccgggcgcg ggcggcaccg tgaacagggc ggagaaccgc tcgaagtagc gggccacggt
      601 ctgctcgagc aggtcgtcga ggttctcgaa gtagcggaag accgacgaca ccgagacgcc
      661 ggcgcgcacg gcgatctcct cgacgccagg gggcgagtgg cgctcgttga tcagctcgaa
      721 cacggcgtcg acgacggcga cgcggttccg ctcacgacgg gctcgccgcc cgtcggtggt
      781 gacgatctgc tcgggcacga atccaccgta gcggccccgc agctcctgcg agtctgactc
      841 ccagaagata ttgacagtac gactctcagg tgtttccatg ggggagaccg gggaggtcca
      901 ggtgcgagca gacgagttcc ggggccgcat cggcccgacc atcgcggagt ccgagccgtg
      961 gttcgacgag cagccgcacc cgggggaggg ggcaccgaac gtggtggtgg tgctcctcga
     1021 cgacaccggc tacgcccagc tcggctgcta cggctccgac atcgacaccc cgaacatcga
     1081 tgccctcgct gcgggcggga tccagttcac gaacttccac gtgaccccgc tctgctcgcc
     1141 gacccgggca tccctgctga cggggcgggc cccgcacgcc gtcgggatgc gctcggtgtc
     1201 gaacttccgc accgggttcc ccaaccagcg cggccacatc tcgaaccacg catgcaccgt
     1261 cgccgaggtc ctccacgacc agggctacgc cacgttctgc gtcggcaagt ggcacctggc
     1321 gcccatggag cagtgctcgg ccgcagggcc cttcgaccag tggcccctcg ctcgcggctt
     1381 cgaccgcttc tacggcttcc tcgaaggcga gaccgatcag ttccaccccg agctcgtcgc
     1441 cgacaaccac ccggtcgatc cacccggtgg gccggaggac ggctaccacc tgtccgagga
     1501 cctggtcgac cagaccctgc ggatgatcgc cgactccacg ggcgtgcgcc ccgaccggcc
     1561 cttcttcacc tacctcgcct tcggcgccac gcacgccccc caccaggccc caccggactt
     1621 cctcgagaag taccgcgggc gcttcgacga gggctgggac gtggtccggg agcgctggtt
     1681 ccaccgccag ctggagctcg gcgtgatccc acccgacacc cgcctcgcgc cccgcaaccc
     1741 cggcgtcgag ccgtgggacg agctgcccga cgaccagcgt cgcctggctg ctcggctgca
     1801 ggaggccttc gccgcgttcc tcgaccacac cgacgtccag atcggccggt tcgtcgacgg
     1861 actgcgccgg ctcggtcagc tcgagaacac gatcgtggtc gtcctcgccg acaacggcgc
     1921 ctcgcaggag ggcgggccct acggcgtgct ccacgagatg aagttcttca acggcatcct
     1981 ggaggcaccc gccgaggccg tggctcacat cgacgacatc ggtggacccc gcagccacac
     2041 caactacccg tggggctggg cgcagtgcgg caacgcaccc ttccgctggt acaagcagaa
     2101 cacccacgag ggcggggtcc acgtcccgat ggtggtgcac tggcccgcag gcatcgacgc
     2161 cgcccagcac ggcacgaagc gtgaccagtt cgccttcgtg gccgacatcg tgccgacgct
     2221 ctacgacgtg gtcggcatcg atccgccggg ggtgcgcaac ggcctcgagc agctcccggt
     2281 gaccgggcac tccttcgccg ccgcgctgca cgacccggcg gcaccggcga cgaacacggt
     2341 gcagtacttc gagaacgccg gcagccgggc catcatcgcc gacgggtgga aggcggtgac
     2401 caagcacgtg gccggcgccg actacgacac cgaaccgtgg gagctgcacc acctggccgc
     2461 ggacccgtcc gagaccgagg acctcgccga gcgcgagccg cagcggttgc aggcgatggt
     2521 ccggctctgg tgggccgagg ccgagcgcca cggcgtgctc cctctcgacg accggctggt
     2581 cgagctgttc ggcgcccgct tccgcgagcg gtccccgcac ccgcccgacc gtcgctacgt
     2641 gtatcggccg cccatgtcgc ccctgccggg gcaggcgggt gctgcgatcg ccggccgcag
     2701 cttcgacctg acggccgccg tcacccacga ccccgcggcg gagggtgtgc tgttcgccac
     2761 cggcaccgag aacgccgggc tctccctctt cgtgcagggc ggccgcctgg tcctcgacta
     2821 caacgcgttc ggcgaccaca cggtcgtgga gtccgacgtg gcgatcccct ccggcgacgt
     2881 ccacctcggg gtccggctcc gccggctcga cggcttcgcc ggcacggcca cgctgaccgt
     2941 cggcggcgcc gacgccggcc acgccgagct ctcgctgctc atgcgcatga tgtcgtccat
     3001 cgggccgagc atcggggccg accacggctc ggccgtctcc gaccgctacg ccgccccgtt
     3061 cccctacggc ggcaccctcc acgagatcgt catccaggcc agccccgacc ggtacggcga
     3121 caccgccgcc accagcgctc gcgctgagat gtcccgccag tagccgctga caccccacca
     3181 ggagcaccag ttgcagaccc tccgcacccc cgacggcgcc ttcgccgacc tgcccgactt
     3241 ccccttcgag ccccactacg tggaggtcga cgacggggag ggcgccacgt tgcgcatcca
     3301 ccacctggac gaggggccgg ccgacgccgc acccgtcctg ttgatgcacg gcgagcccac
     3361 gtggagctac ctctaccgcc acgtcatccc gggcctggtc gaggccggcc accgggtgat
     3421 cgccccggac ctggtcggca tgggccgcag cgacaagccc accgagcagg ccgaccacac
     3481 ctacgcccgc cacgtcgagt ggatgcgcca ggccctcttc gaccggctcg acctgcggga
     3541 catcacgttc ttcggccagg actgggggtc gttggtgggc ctccgcctcg tcgcgctcga
     3601 ccccgaccgc tacgcccggg tcgcgatcgg gaacggtggc ctgcccaccg gccacggtcc
     3661 ggcgagcgag gcgttcctgg cctggcagcg gttctcccag gagaccgagc acttcccggt
     3721 cgggcgcatc atcgacggcg ggtgcacgac ctcgctcagc cccgaggtca tcgctgccta
     3781 cgacgcgccc taccccgacg actcctacaa ggccgcggtg cgggtgttcc cgagcctggt
     3841 gcccaccagc tccgacgatc cggccacacc ggaccaggag gccgcgtggc gggtgctcga
     3901 gcgcttcgag aagccgttcc tgctgtgctt cagcgactcc gacccgatca ccaagggtgg
     3961 cgatgcgccg ttcctggcca aggtgcccgg cgcccagggc caggcccaca ccacgatcga
     4021 gggtggtggg cacttcctgc aggaggaccg cggtccggag cttgcccgcg tgctcgccgg
     4081 cttcatcgag gccacgtcgt gatcctcgac gacgcaggct ggcgggccct caccgccgac
     4141 gccgccctgg cgtcgcaccg tctcatcggc tggatctact gggatcccac cgccgtggcg
     4201 aactacgcag cgctcggggt gcccgacggc gccggctact acttcgccac ccgtgctgcc
     4261 tcgatcgccc aggccggcga cgacgtcgtg accgccacct tcggctccat ccatccggcg
     4321 ttcatctcgg cctgcctcga cctgtgccgc cagcacacga ccttcgaggc cgccgccctc
     4381 gctcgtgacg ctgccgtcga gcccggcctg cgggccctgg cccccgacct gtgcgacggc
     4441 ctcaccgagc tcggcccgca cctgtgggcc gccgccgacg agctggtgct cacgggtcgt
     4501 cccctcttcg cgtcgctgct gcgccggcgc cgggaggacc agccgctcct gtcggcctgg
     4561 ctcgcggtca actgcatccg cgagtggcgc ggcgacacgc actgggcgat ccacgcggtc
     4621 gagggcatcg acgggaccat ggccggcctg ctcgacgggg cgtggcgcgg ccacgacgac
     4681 gactggctgc cccgctcccg gggtgctgac gacgcgctgc tcgacgccgc cctcgccgag
     4741 ctcgaggctc gcggcctggc caccgacggg cgggtgaacg ccaccggcgt cgcccaccgc
     4801 caacagctgg aggaccgcct ggacgacctg tgcacgcctg gctggcaggc cctgggtgaa
     4861 ccgctcaccc gccgtctcgt ggagctgatc gagccgtacg ggtccacgtt cgtcgagcgc
     4921 atcgaccaga ccgccggtcc ccggtggatg ccggcggcac gggaccggac cgtgcgggcg
     4981 tgacggccac ctcgtcgcgg acgagcaccg tcgcgacctc gcccgaggcg gtgtgggagg
     5041 tcctcgccga cttcggcgcg ctcgcccggt gggtgcccga ggtggatcac tcctgcctgc
     5101 tccgcagcgg gccggcgggc gtcggcacga cccggcgggt ccaggtcggg cgcacgaccg
     5161 tgctcgaggc catcaccgcg tggtcgccgc ccgagcacct cggctacgcc atcaccggcc
     5221 tgccgccggc gctgcagacg gtgcggaacg actggcgcct ccgcccggtc gccgatggca
     5281 cggaggtcac cgtcaccacg agcgtcgacg ccggcccccg accaccccag cggttggtcg
     5341 cccatctcgt cgctcgccgc ctgtcgacga cgtcgggtca gatgctcgcc ggcctcacgg
     5401 ccaccctcac ctccggagac cacgcccgtg cctgagaccc gtcccgacgt cgtcgtgctg
     5461 ctgacggacg aggagcgcgc cgcgccgccg tacgagaccg aggcgctgcg ggcgtggcgc
     5521 cggcgcgccc tgcccggggc gcggtggttc gaggagcacg gcgtctcctt cgcccgccac
     5581 cacaccggat cgctcgcctg cgtgccgagc cgcccgacct tgctcaccgg ccagtacccc
     5641 gacctgcacg gcgtcagcca gaccgatggc ctcggcaaga tggcggacga ctcgcgcatg
     5701 cggtggctgc cgcccgagga ggtgcccacg gtcggcagct ggttccgcgc cgccggctac
     5761 gacacgcact acgacggcaa gtggcacgtc tcccacgccg atctcaccga tccgacgggt
     5821 gccccgctcg ccaccaacga cgcagagggc aacgtcgacc cggacgccgt gcaggcctac
     5881 ctggacgccg acccgctcgg cccgtacggc ttctccggct gggtcggccc cgaaccgcac
     5941 ggtgcgccga tggccaacag cggcctggtg cgcgacccgc tcatcgccga ccgggtggtg
     6001 gcctggctgg aggaccgcta cgcccgtcgc cgggccggcg atcccgaggc gctgcgcccc
     6061 ttcctgctgg tcgccagctt cgtgaacccc cacgacatcg tgctgttccc ggcgtgggtg
     6121 cggggccgct ggccgctcga gccgtctccg gtcgaccccc cgtccgtgcc gccgtcgccg
     6181 accgacgacg aggacctgcg cgacaagccc gccgcccagg tcgcctaccg ggccgcctac
     6241 ccctcggggt acggaccggc cccggcggtg gcccgcatct accggcgtgg ggcgcagcgc
     6301 taccgcgacc tgtaccaccg cctgcacgcc gaggtcgacg gaccgctcga ccgggtccgc
     6361 cgagccgtca ccgaacaggg ttccgagcac gcagtgctgg tccgcaccag cgaccacggc
     6421 gagctgctcg gcgcccacgg ggggctgcac cagaaatggt tcaacctcta cgacgaggcg
     6481 acccgcgtcc ccttcgtcgt cgcccgggtg ggcgagcgcg ccaccgaggc gcgcacggtc
     6541 gacgcgccga cctcccacgt cgacctggtg ccgaccctgc tggccgcggc cggcatcgac
     6601 gtcgacgcca ccgcacgcga gctggcctcc acgttctcgg aggtccaccc gctgcccggg
     6661 cgcgacctgc tgccggtggt ggacggtgcc gaggccgacc cggatcgggt cgtctacctc
     6721 cagacgcggg acaacatgct cgagggcgac acgggtgcgt cgggggtggc ccgccggctc
     6781 ggccgcacgg agcggccccc ggcaccgctg cggatccgcg tgccggcgca cgtcgcctcc
     6841 agcttcgaag gggtcgtggc gcgcgtccgg gacgaggtcg ccgccggtgg tgccgggcac
     6901 cgttggaagc tcgtgcgcac gttcgacgac ccgagcacct ggaccgaacc cggcgtgcgc
     6961 cacctggccg acacgggacc gggcgggccg acgtaccgga ccgagccgct cgaggaccag
     7021 tgggagctct acgacctcga cgccgatccg gtcgagatcc gcaaccgctg gcggcacccg
     7081 gatgccaccg ccgtgctcga gcacctccgt gacgtgctga aggccgaggc ctcacgcagc
     7141 atccccgagc gcaaccggcc gctgacgccc ctccggtggc accccgccgg ccatccggcg
     7201 aagaccccgc ctcggccggc cctcgcgctg cgccgcctcg tccagcgcct cggcatgcac
     7261 ccggaggaca cggacgccga ccacagcgtc gaccgctccg ggcgccgcgc gctcgtcgtg
     7321 tgcacgaacc acggcgtgct cgacgtcggc aagcccacgg gcgtgttcgc cagcgagatg
     7381 acggtgccgt actacgcctt ccgcgacgcc ggcatggtgg tcgacatcgc cagcccggcc
     7441 ggtgggaccg tgccgatcga cccgcagtcg ctcacgccgg tgctgcgctg cgggtccgac
     7501 gaccgcttcc tcgctgacgc agagctgcgg gcgaaggtga ccgactcgct gtccatcgcc
     7561 gacctcgaca tcgccggcta cgacctcgtg tacctcgccg gcgggtgggg tgccgccttc
     7621 gacttcggca cgtcccaggc gctcggcgac cagctgagcc gggccgagga gctcgggctg
     7681 cccatcgggg gcgtgtgcca cggcccgctc gcgctgcggc tggcgaccgc ggccgacggc
     7741 cgaccgctcg tcgagggccg gcgcatcagc tgcgtcaccg acaagcaggt gcacgagctg
     7801 ggcatcgcct cgacgccgat gcaccccgag accgagctgc gcgcccttgg cgcacggttc
     7861 gaggcccggc accggttccg cgaccccttc gccaaccact gggtggtcga cgggaacctc
     7921 gtcaccggcc agaaccagaa cgcgggcccc atggtcgccc gggagctgct cgccctcgtg
     7981 ccggccgacc cacgatcgga gccccgcaca tgaccgacac ccgcacaccc ggctacggcc
     8041 aggtcgaccg cgactacggc atccgcctgg ccaccacccc gccgggggcg gacggcccca
     8101 tctggatggt gaacctcatg tcgtaccggg ggcgggcggt ctacgacggc gccgaggccg
     8161 acgtgaccgg ccgcgaggcc gacgaccgct acgcgccggt cgacgtcctg gccgacatcg
     8221 gcgcccaggt cgccttcgtg gccgacgtgg aggaccagct cctcggcgac tcgccccggt
     8281 gggaccgcat cggcgtcgtg cgctacccga cgcgccggtc gttcatcgag atgcagtccc
     8341 gtcccgactt caaggccaag cacgtgcaca aggaggcggg catggccgag acgatcgtcg
     8401 ccgggtgcac gcccatcgcg tcgccgcagg atcggcccga tgcgccgacc cccgtcgact
     8461 ggtcgtcggt gccgcaccca ccgacggatg cggacgggcc cgtggtggtg ctgcacctga
     8521 tccgcttcca ccccggcggg gccgacaccg acatggtgcg ctatcaggac gacgccgcgc
     8581 gcgtggcggt gccccacggc gtgcgcatcg cggggtggtt cggcgtcgag ggcaccatcg
     8641 tcggtgacgg ccgccgctgg gaccaggtcc ggttcaaccg gttcccctcg aaggcagcgt
     8701 tcctcgccgt ggccacggat ccgggtcggc tcgagtccca ggccgcccac cgagaggctg
     8761 ccatcgcgga cacctacgcc ctgatcctgc ggccgaccat cgatcggctg gacgcctcgg
     8821 tggccgaggt cggcgggatc agctgacgcc ggagagcgcc gggtccagct cgtagacccg
     8881 gtagctgctg cggatcacgg gcatcgccac gttgcggacc cgggtgccgc cggcggtcat
     8941 gccgtgctcc acgccggcgt gggcgtgacc gtgcagggcc aggtcggccc cggcgccgtc
     9001 gatggcctcg gccagcaggt agctgccgag gaacgggaag atctcgaggc gctcgccccg
     9061 cagcgtgtcc tccacggggg agtagtggag gagcgcgatg cgcaggtcgg tgtcgagtgc
     9121 ggcgagcgtg tggcagagct ggtcggcgat gcccttggtg tggcggacga acgccttcat
     9181 ctccggctcg ccgaagtcgc tggcgcaggc acccgcgaag ccgcccccga agcccttggc
     9241 cccggcgatg ccgacggtgg tgccgtcgac gtccaccacg gtggaggagc cctccaggac
     9301 ggtgatgccg ctggacgtca gctggtcggc gaaggcatcg gcgcacccgg cgtggtggtc
     9361 gtggttgccc agcaccgcca cgaccggcat gtccaggtcc gacaccgacc ggcagaccag
     9421 ctcggcctcc tcgacctcgc cgtgtcggct gaggtcgccg gcgagcagga ggaggtcggc
     9481 gtcgtcctcg gcggtgcgga ccgactcgcg gaacgcgtcc acgttgtcgg ccccgaggtg
     9541 gatgtcgccg acggcggcga tccggatcat cggatgcgct ccggggtgtg ctcgccgtcg
     9601 agcggcacga ccgtgacgtc gttgtggatg cgccggtccg ggacgacctc ggccgccacc
     9661 tcggcgacgg cggcccgtcg ctcggcgctg gcgacctcac cgaagaggaa cacctcgacc
     9721 tccgtgaccc gcacctgcac gccctgctcg gtggtgcggg ggtcggtggt cacggcctgg
     9781 tgcacccgct cggcgaggta ctcggggctg tcgtccatcg gccgctcgct catgccacca
     9841 cacccccgtg gatggtgtcg tgcatcgagg tgatggcggt tgtcggcacg gccaggtcgc
     9901 aggactcggc gtagatcagc aggctcagca cgcgtcgggg accggcctgg ttggcccgac
     9961 ggacgaggta gtcccagtcg aggtcgcact tggcgatgag ggcgagggcg tcgtaccagt
    10021 ggtgggccgt ggtctccccg gtggccagcg ccttgatgac cagcaggtcc tcggcggaga
    10081 tgacccgggc gctcacgccc ttgtagtcgc gccgctcgga ccgctccagc atctcgtcgt
    10141 cgaggtagat ctcgcccgac gaccggaaga tcaggtcgac cagcacgcgc cgcttgaacg
    10201 ccttcgccag ccaggcgggg tcgtggatcg tggtctcgaa cccgtcgtgc tcgagcaggt
    10261 cgagcgcctt ctcgacgtca cccgggcgga ggaagacgtc gatgtcgtgg gtctcgcggg
    10321 gacgggccat ggcgaaggag ccgatgccgc ccatcagcag gtggtcggtg tcgctcgcct
    10381 ccaggcagcg caccacgtcg ccgagggtgt cgagcaggtg ctgctcgtcc gggacgtgac
    10441 cgtccacgta gtgatcggtc gcgcacggct gatcgaagcg ggatgctggc acccgctacg
    10501 gataccccca gccgccgccc ttcatgcgat cgggacgcct ccgacgacga cggccaggga
    10561 caccgaggcc cacacggcga tcgaggcggg gagacgtcgc cccctggggg cgctcagtgc
    10621 catggcgccg caggcgaggg ggagcgcggc caggtacgcc gccgggatga tcacgactgc
    10681 cagggcgacg agcgcggtca cggcgagggc gagcacctcg gctcggcgcg tcagctgggc
    10741 tccggcgatg gcgaccaccc gagggtcatg ccccgcaccg gcccgctcag acctggatca
    10801 gcgaccggat cggtgggatc ccggggcgcg cgtgcggttg cggcccgtcg gcggacgcat
    10861 ccgacacgga ccgtgcctca gccgccccgc acggtgcggc ccgatcgcta cgcgagcggt
    10921 cggccgaagg ggagctcgtc gcgccaggtg cgcaggaagg cctcggcctc ctcgacggcg
    10981 gcgagccaca tgccggtgcc gtgggacgcg aggccggcga ccttgtcggc gacggcagcc
    11041 acggggtcgg tgacgtcgtc gccgtgggcg tggagcaggg gcgcagcagt ggagaggaag
    11101 tcgggcacgt gcaccgtgcc ggccttggtc agctcggcgt gggcccgggc ggtgaccggc
    11161 acgggcgtga gcggcacgac cacgccggcg gtgatgtcgg ggacgacctc gtggtcgagt
    11221 acgccggtct tgccggcgac gaagaggacg tcggcctgcg tggcggggga gtcgccctcg
    11281 gccaccgtgc caccggcggc ctcgaccgcg gcgcgggcgg cgtcgacgac gggacccttc
    11341 ccgacgaagg cgcacgtggc gccggtcagg ccaccgtcgc gggcggcgcc ggcggccgcc
    11401 accgcgccct gggcggtgag ggtctcggcg agaccgtcgg tccacagctc ggacggccgg
    11461 cggtcgtcga tgcgcagctg ggcgaggtcg gcctcggcga gaccgagcgc ggggtcggtg
    11521 gcccagcgtc cctcggcgac ggcggatcgc atctcctcca cgaaggccgc cacggccgga
    11581 tcgcggtcgt cgccctcggc gttgatgccg gcggaaccgc cgcccatctg gatgccgaag
    11641 ctggcgaacg cgtaggtggt ggagcgggcg aggagctcgg cgccgtcggt caggaccttg
    11701 cgggccaacc gggtgacgcc gacggacggg gcgtcgtcga ggtcgaaggc gacgaaggca
    11761 tcggtggacg acagcttgtg gatgcgcacg acggcgattc tggcacgggg ccgacgccgc
    11821 gctcgtgggc gccggggctg tcggaggggg ccggtagggt cgccacggtg cctgcgtcga
    11881 ccgaggaacc cctgccgagc gagccggcgg accagctctt ccccgagccg accgaccgca
    11941 tcggccccgg ctccggaccg ttcggcgcgc ccctgctcga cggcctgaac ccggcccagg
    12001 aggaggcggt cacccacctc ggcgggccgc tgctcgtgct cgccggcgcc ggctcgggca
    12061 agacccgcgt gctcacccgt cgcatcgcgt ggctcatcca ggagcagggc gtcagcccgt
    12121 tcgagatcct ggccatcacc ttcaccaaca aggccgccga cgagatgaag gcccgcgtcg
    12181 gcaccctcgt cggctcggtc gcccagaaga tgtgggtgtc cacgttccac tccgcctgcg
    12241 tgcgcatcct gcgccgcgac gccggtgcca tcggcttccc gtcgtcgttc accatctacg
    12301 accaggccga tgccgtccgc ctcaccggct acgtcctgcg cgacctgaac ctcgacacca
    12361 agaagatgcc gccccgctcg gtccacgccc agatcagcgc ggcgaagaac gactacgtcc
    12421 tcgtcgacga gtacgccgac cgggccgggt ccttcaccga gcgcaagctg gccgacgtct
    12481 accgcgagta ccaggcccgc ctgctccgcg ccggcgccat ggacttcgac gacatcctcc
    12541 tcaacgccgt cgagctgttc cgccgccacc ccgacgtgct cgcccactac cgccagcgct
    12601 tcacccacgt cctcgtcgac gagtaccagg acaccaacaa ggtccagaac gagctcgtgg
    12661 tccagctggc caaggagcac cgcaacgtca cggtcgtggg cgacggggat cagt
//
LOCUS       DATISU010000036         7368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_840089, whole genome shotgun sequence.
ACCESSION   DATISU010000036 DATISU010000000
VERSION     DATISU010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7368
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840089"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1670
                     /locus_tag="VLR27_01780"
     CDS             165..1670
                     /locus_tag="VLR27_01780"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HSP02201.1"
                     /translation="MPAAPRLSPVRLLLVVLAATLALLGCSDDDAGPTATSTEPTTTS
                     TTTSTTAPAPPRPDVTVTGPVPGTPQTSAAAGLEEAGYVEEEFFVSGTAQAFDAEDEL
                     GDDGRWSLTVREEAPYTTRILVKRPAEAADASGVVVVEWNNVSAGSDGTPDWTFTAAE
                     LLRAGHVHVAVSAQAAGVDAGEGGILGGGGNPLTAADPERYGALVHPGDDHSYDLFAQ
                     IGALVSSEPDGGVDPLAGLPRDHVLAIGESQSAFRLTTFVNGVHPLRPVFDGFLVHSR
                     GGGAAPVDASAEEGLASSIAGEVRIRDDLDVPVLVFSTETDLTMLGYAPARQPDSEHV
                     RSWEIAGTAHADAFLLGGDPVAAGEALGCAAPVNDGPQHLALKAALAHLVEWVVDGTE
                     PPAGDPIEVGDDGAIVRDEAGNAVGGIRLPAVAVPAATLSGDAAGGSVVCQLFGSTTP
                     FPIEVLVERYGGRAGYLEQYEDALDRAIADGFVLEADRATALAEAEEVDFG"
     gene            complement(1747..2637)
                     /locus_tag="VLR27_01785"
     CDS             complement(1747..2637)
                     /locus_tag="VLR27_01785"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSP02202.1"
                     /translation="MIPTMPFGRTGHDSTRVLFGAAALGAMSQQRADDTLELITRHGV
                     NHIDTAASYGDSELRLAPWLADHRAGVFLATKTGERTGAAARDELERSLTRLGVDHVD
                     LIQLHNLVEEDEWQVAFAPGGVVEAMSAARDEGLVRHIGVTGHGMRIAGMHQRSLEAF
                     PFDSVLLPFNAVLLRDPGYRADVEALRSTCRERDVAVQTIKAIARRRWRDDDESRRLA
                     WYEPLRDPAAIDRAVRFVLAQPDLFLNTTSDATLLPLVLEAASRLDQPFAAPTDAELD
                     ADIDGLGIEPIFDGGALERI"
     gene            2691..3260
                     /locus_tag="VLR27_01790"
     CDS             2691..3260
                     /locus_tag="VLR27_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSP02203.1"
                     /translation="MAEVLLFHHALGLTDGVRALADDLRAAGHVVHLPDLYDGRTYTE
                     LGEGVEHAEELGFDTIRARGAAAAEDLPAELVYVGLSLGVMPAQELAQTRAGASGAVL
                     LHACIPLDFFGDGSWPADVPAQVHVMEDDDLGDVDIARDVAATTDPFELFVYPGDRHL
                     FTDRSTPDHDADAASLALRRVLAFLDEVG"
     gene            complement(3267..4379)
                     /gene="prfB"
                     /locus_tag="VLR27_01795"
     CDS             complement(3267..4379)
                     /gene="prfB"
                     /locus_tag="VLR27_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HSP02204.1"
                     /translation="MRDFSDDLNDLRRRLDEAATYLRIEELRARLPQLETEAGRPDLW
                     DDQERAKEVNAELSATTDDLDAFDALAGRIEDAETLAEMAREEGDESLEPEIAQAITE
                     IEGDFDRIELRSLFSGEHDERDAICELHSGEGGADASDWAEMLLRMYQRWAANHGFEV
                     EMEAVTEGTEAGISSATFIVKGRWAYGWLRSEHGVHRLVRISPFNSQGKRQTAFAALE
                     VVPLLDEAETDVEIDDKELRIDVYRSSGAGGQHVNKTDSAVRLTHLPTGIVVACQNER
                     SQHQNKDRAMTMLKAKLAELERQKRVDELASIKGEQRSVGFGSQIRSYVLQPYQMVKD
                     LRSDFEVGQVDTVLDGDLDRFMEAFLRWERTEGLES"
     gene            4510..5916
                     /locus_tag="VLR27_01800"
     CDS             4510..5916
                     /locus_tag="VLR27_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HSP02205.1"
                     /translation="MVTDRFMEPTDAWIWYLEQDPLLRFTVTAVTVLDRAPDWDRLVD
                     RFEKATRVAPHFRDRVVSTPFRLAPPRWVTDKSFDLGYHLRRMRAPEPGTLESVLELA
                     RTTAMAGLDRSRPLWEATLVEGVDGDGAALVIKAHHSLTDGVGGMQLALVLFDLEPDA
                     DPTDWPDAPVPEALTTTDLAWQATVHNATRAAELGARTVGAALPTLGRLAREPVATTR
                     GWLRSGGSIARMVRPVGATLSPVMADRGLSWRFRVLDVPFDELRGAAKATGTTLNDAF
                     LAGITGGMRRYHEQLGHPVAELRVSMPISTRKDGDPVGGNRVAIVRYPVPVDIADPQA
                     RLHALHEAAARARNEPAVPHTDAILEVVSPVTPLAVRAMAVHMDFGASNVPGPAFPVH
                     LAGAEALRFYPFGPTGGSAVNITLLSYRGTCCIGVNMDTAAITDGDRFLACLADGFAE
                     VLAVGDGEPAVTPARGRA"
     gene            complement(5891..>7368)
                     /locus_tag="VLR27_01805"
     CDS             complement(5891..>7368)
                     /locus_tag="VLR27_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HSP02206.1"
                     /translation="SRTLDKRGVKHQVLNAKPERQFREADIVAQAGRLNTVTVATNMA
                     GRGVDILLGGNPELLAKQELHNEGLDADDPENAERLAKTIAKYEPQCQAEGDKVRALG
                     GLYVLGTERHDSRRIDDQLRGRAGRQGDPGESRYYLSLEDDLMRLFATGAVNWVMGKA
                     LPDDVPLEAKMVTKAIERAQQTVEQRNAEIRKNVLKYDEVLNSQRKVIYELRDQILDK
                     TDLRAKIHEKYLPEAVESLVATYCVADFSEEWDLEGLVAAAGEYWPSEVTVEQLAALS
                     TSDEVYAVLVDEAISRYEAREAELGDDTMRQVERQVMLRIIDTAWREHLREMDYLQEG
                     INLRAMGQRDPLTEWQREGFEMFEAMIARIERDLVRYVMHVQVKAPGGSEQAQPAAPA
                     VGMQFQTSGDKAPAAPLPDDEPAVSNVQTSGPADPSSQTGGLSTLARTRAQVEGAPKQ
                     VDEAAAASHTPVVKSEWDKTPRNAPCPCGSGKKFKLCHGQA"
BASE COUNT          926 a         2688 c         2666 g         1088 t
ORIGIN      
        1 gcgaggagca gggacagggg ctttcgcacg gtaccaacga ccccctgggg gatcggggac
       61 acccgggacg ggacgtccgg gcacgttgcg aacaggttac gaaggctaca cggggtcccc
      121 acgggcggac gaagccgcca cggccgatgt gggagcctgc gacggtgccg gccgcccctc
      181 gcctctcgcc cgtccgcctc ctcctcgttg tcctcgccgc caccctcgcc ctcctcggct
      241 gcagcgacga cgacgcagga ccgacggcga cctcgaccga gccgacgacc accagcacca
      301 cgacgtcgac cacggcgccg gcgccgcccc gacccgacgt cacggtgacc gggccggtgc
      361 cggggacccc gcagacgtcg gccgcagccg ggctcgagga ggccggctac gtcgaggagg
      421 agttcttcgt cagcgggacc gcccaggcct tcgatgccga ggacgagctc ggcgacgacg
      481 gccgctggtc gctgaccgtg cgggaggagg cgccctacac cacccgcatc ctggtcaagc
      541 ggcccgccga ggccgccgac gccagcggcg tggtcgtggt cgagtggaac aacgtgtccg
      601 cgggctccga cggcaccccg gactggacct tcaccgccgc cgagctgctc cgggccgggc
      661 acgtgcacgt ggcggtgtcg gcccaggccg ccggcgtgga cgccggcgag ggcggcatcc
      721 tcggcggcgg cggcaacccc ctcaccgccg ccgaccccga gcgctacgga gcgctggtgc
      781 accccggcga cgaccactcc tacgacctgt tcgcccagat cggcgccctc gtctcgagcg
      841 aaccggacgg gggcgtcgac cccctggccg gtctcccccg ggaccacgtc ctcgccatcg
      901 gcgagtccca gtcggcgttc cgcctcacga ccttcgtcaa cggcgtccac ccgctgcgcc
      961 ccgtgttcga cggcttcctc gtgcacagcc gaggcggcgg agcggcaccg gtcgacgcat
     1021 cggccgagga gggcctggcc tcgtccatcg ccggcgaggt gcgcatccgg gacgacctcg
     1081 acgtgcccgt gctcgtgttc tcgaccgaga ccgacctgac catgctcggc tacgcgccgg
     1141 ctcgccagcc cgacagcgag cacgtccgct cgtgggagat cgccggcacg gcgcacgccg
     1201 acgcgttcct cctcggcggc gacccggtgg cggccggcga ggcgctcggc tgcgccgcac
     1261 cggtgaacga cgggccccag cacctggccc tgaaggccgc gctggcccac ctcgtcgagt
     1321 gggtcgtcga cggcaccgaa cccccagccg gcgaccccat cgaggtcggc gacgacgggg
     1381 ccatcgtgcg cgacgaggcc ggcaacgccg tgggcggcat ccggctcccg gccgtggcgg
     1441 tgccggcggc cacgctgtcc ggcgacgccg ccgggggcag cgtcgtctgc cagctgttcg
     1501 gctccaccac gccgttcccg atcgaggtgc tcgtcgagcg ctacggcggc cgggccggct
     1561 acctcgagca gtacgaggac gcgctcgacc gggccatcgc cgacgggttc gtgctcgagg
     1621 ccgatcgggc caccgccctc gccgaggccg aggaggtcga cttcggctga ccgccgggcc
     1681 tgctccgatc tccgttctgg gatgctccac acgccgtccc tgcgtgtacc gcggcccaga
     1741 acgggatcag atgcgctcga gcgcgccgcc gtcgaagatc ggctcgatgc cgaggccgtc
     1801 gatgtcggcg tcgagctcgg cgtcggtggg tgccgcgaag ggctggtcga gccgggacgc
     1861 ggcctcgagg accagcggga ggagcgtggc gtcgctggtg gtgttcagga acaggtccgg
     1921 ttgggcgagc acgaagcgca cggcccggtc gatggccgcc gggtcgcgga ggggctcgta
     1981 ccaggcgagc cgccggctct cgtcgtcgtc ccgccagcgg cgacgggcga tggccttgat
     2041 cgtctgcacg gcgacgtctc gctcccggca cgtcgaccgc agcgcttcga cgtcggctcg
     2101 gtagcccgga tcccggagca ggacggcgtt gaagggcagg agcacggagt cgaaggggaa
     2161 cgcctcgagg ctgcgctggt gcatgccggc gatgcgcatc ccgtgcccgg tgaccccgat
     2221 gtggcgcacg aggccctcat cccgtgccgc cgacatggcc tcgaccacac cgcccggcgc
     2281 gaaggccacc tgccactcgt cctcctcgac caggttgtgg agctggatga ggtccacgtg
     2341 gtcgaccccc aaccgggtga gggatcgctc cagctcgtcc cgggccgcgg cgccggtgcg
     2401 ctcgccggtc ttggtggcga ggaagacgcc ggcgcggtgg tcggcgagcc agggcgccag
     2461 ccgcagctcg gagtcgccgt agctggcggc ggtgtcgatg tggttcaccc cgtggcgggt
     2521 gatcagctcg agggtgtcat cggcccgctg ctggctcatg gccccgaggg cagccgcccc
     2581 gaagagcacg cgggtgctgt cgtgcccggt ccggccgaag ggcatcgtcg ggatcatggg
     2641 gtcctgtgta ccccagcagg gcacgtcctg tccccggtac cgtcgccggg atggccgagg
     2701 tcctgctgtt ccaccacgcg ctggggctca ccgacggcgt ccgggcgctg gccgacgacc
     2761 tccgggcggc cggccacgtc gtccacctgc ccgacctcta cgacgggcgg acctacaccg
     2821 agctcggtga gggcgtcgag cacgccgagg agctcgggtt cgacaccatc cgggcacgtg
     2881 gggcggccgc cgccgaggac ctgccggccg agctggtgta cgtcgggctg tcgctcggcg
     2941 tgatgccggc ccaggagctg gcccagaccc gggccggtgc gagcggtgcc gtgctgctcc
     3001 acgcctgcat cccgctcgac ttcttcggcg acggctcctg gcccgccgac gtgccggccc
     3061 aggtccacgt catggaggac gacgacctcg gcgacgtcga catcgcccgc gacgtcgcgg
     3121 ccaccaccga tccgttcgag ctgttcgtct accccggcga ccggcacctg ttcaccgacc
     3181 ggagcacccc ggaccacgac gccgacgcag catcgctcgc gctccggcgg gtgctggcct
     3241 tcctcgacga ggtgggctga cgcgactcag ctctcgaggc cctcggtgcg ctcccagcgc
     3301 aggaaggcct ccatgaaccg gtcgaggtcg ccgtcgagga cggtgtcgac ctgcccgacc
     3361 tcgaagtcgg accgcaggtc cttgaccatc tggtacggct gcagcacgta ggaccggatc
     3421 tgggagccga agccgacgct gcgctgctcg cccttgatgg acgccagctc gtcgacccgc
     3481 ttctggcgct cgagctcggc cagcttggcc ttgagcatcg tcatggcccg gtccttgttc
     3541 tggtgctggc tgcgctcgtt ctggcaggcc acgacgatgc ccgtgggcag gtgggtgagg
     3601 cgcacggccg agtcggtctt gttgacgtgc tggccgccgg cgccggagga gcggtacacg
     3661 tcgatgcgca gctccttgtc gtcgatctcc acgtcggtct cggcctcgtc cagcagcggc
     3721 acgacctcga gggcggcgaa ggcggtctgg cgcttgccct gggagttgaa cgggctgatc
     3781 cgcaccagcc ggtgcacccc gtgctcggag cgcagccagc cgtaggccca ccggcccttc
     3841 acgatgaagg tggccgagga gatcccggcc tcggtgccct cggtgacggc ctccatctcg
     3901 acctcgaagc cgtggttggc ggcccagcgc tggtacatgc gcagcagcat ctcggcccag
     3961 tcggacgcat cggccccgcc ctcgccggag tgcagctcgc agatggcatc gcgctcgtcg
     4021 tgctcaccgg agaacagcga gcgcagctcg atgcggtcga agtcgccttc gatctccgtg
     4081 atggcctggg cgatctcggg ctccaggctc tcgtcgccct cctcgcgggc catctcggcc
     4141 agcgtctcgg cgtcctcgat gcggccggcg agcgcgtcga acgcgtccag gtcgtcggtg
     4201 gtggccgaca gctcggcgtt gacctccttg gcccgctcct ggtcgtccca gaggtcgggg
     4261 cggccggcct cggtctcgag ctggggcagg cgggcccgca gctcctcgat ccgcaggtag
     4321 gtcgcggcct cgtcgaggcg gcgccgcagg tcgttgaggt cgtcggagaa gtcgcgcatg
     4381 ggagacgtgc agcctattgg gcacggctgt gggcgccgga tctgaccacg ccgggacgtt
     4441 ctgccctggc gaccgcgcca cccgctgcgc acgatgcggt gagccgccgc cgaacccggg
     4501 agggtcatcg tggtcaccga ccgcttcatg gaacccacgg atgcctggat ctggtacctg
     4561 gagcaggacc cgctcctgcg gttcaccgtc acggcggtga cggtgctcga ccgcgctccg
     4621 gactgggacc gcctggtcga ccgcttcgag aaggccaccc gggtggcgcc gcacttccgg
     4681 gaccgggtcg tctccacgcc gttccgcctg gcgccacccc gctgggtcac cgacaagagc
     4741 ttcgacctgg gctaccacct gcgccggatg cgcgcgcccg agcccgggac gctcgagtcg
     4801 gtcctggagc tggcccgcac gaccgcgatg gccggcctcg accgcagccg gccgttgtgg
     4861 gaggccacgc tggtcgaggg agtcgacggc gacggcgccg cgctcgtgat caaggcccac
     4921 cactcgctga ccgacggcgt cggcggcatg cagctcgccc tcgtcctgtt cgacctggag
     4981 cccgacgccg acccgaccga ctggcccgac gcacccgttc ccgaggcgct gacgacgacg
     5041 gacctggcgt ggcaggcgac ggtccacaac gccacccggg cggccgagct cggcgcgcgc
     5101 acggtcggcg cggcgctgcc caccctgggc cgcctggccc gggagccggt cgccacgacc
     5161 cgcggctggc tccgctccgg tggctccatc gcccggatgg tgcgccccgt cggcgccacc
     5221 ctctcccccg tgatggccga ccgggggctg tcgtggcgct tccgggtgct cgacgtgccg
     5281 ttcgacgagc tgcggggcgc ggccaaggcg acgggcacga cgctgaacga cgccttcctg
     5341 gccggcatca ccggcggcat gcgccgctac cacgagcagc tcggccaccc cgtcgccgag
     5401 ctgcgggtga gcatgccgat cagcacccgc aaggacggcg accccgtcgg cgggaaccgc
     5461 gtggccatcg tgcgctaccc ggtgccggtg gacatcgccg acccgcaggc gcgcctgcac
     5521 gccctccacg aggccgcggc gcgggctcgc aacgagccgg cggtgccgca caccgatgcg
     5581 atcctcgagg tggtctcacc ggtgacgccg ctcgccgtcc gggcgatggc ggtgcacatg
     5641 gacttcgggg cgagcaacgt cccgggcccg gccttccccg tgcacctcgc cggggccgag
     5701 gcgctgcgct tctacccgtt cgggcccacc ggtggctcgg ccgtgaacat cacgctgctc
     5761 tcctaccgcg ggacgtgctg catcggcgtg aacatggaca ccgcggcgat caccgatggc
     5821 gaccgcttcc tcgcctgcct ggccgacggc ttcgccgagg tgctcgccgt cggtgacggc
     5881 gagcccgcgg tcacgcctgc ccgtggcaga gcttgaactt cttgccgctg ccgcaggggc
     5941 agggcgcgtt gcgaggggtc ttgtcccact cggacttcac gaccggggtg tgcgacgcgg
     6001 ctgcggcctc gtcgacctgc ttgggcgcac cctcgacctg ggcccgggtg cgagcgaggg
     6061 tggacagccc gcccgtctgg ctggacgggt cggccgggcc ggaggtctgc acgttggaca
     6121 ccgccggctc gtcgtcgggc agcggggcgg ccggggcctt gtcgcctgac gtctggaact
     6181 gcatgcccac ggccggagcg gcgggctggg cctgctcgga tccgccgggg gccttgacct
     6241 ggacgtgcat gacgtagcgg acgaggtcgc gctcgatgcg ggcgatcatg gcctcgaaca
     6301 tctcgaagcc ctcccgctgc cactcggtga gcggatcccg ctggcccatg gcccgcaggt
     6361 tgatgccctc ctgcaggtag tccatctccc gcaggtgctc gcgccacgcc gtgtcgatga
     6421 tgcgcagcat cacctgacgc tcgacctggc gcatggtgtc gtcgccgagc tcggcctcgc
     6481 gggcctcgta gcggctgatg gcctcgtcga cgaggacggc gtagacctcg tcggaggtgc
     6541 tcagcgcggc gagctgctcg acggtgacct cgctcggcca gtactcgccg gcggcggcca
     6601 ccaggccctc gaggtcccac tcctcgctga agtccgcgac gcagtaggtg gcgacgaggg
     6661 actcgacggc ctcaggcagg tacttctcgt ggatcttggc ccgcaggtcg gtcttgtcga
     6721 ggatctggtc gcgcagctcg tagatgacct tgcgctggct gttgaggacc tcgtcgtact
     6781 tgaggacgtt cttgcggatc tcggcgttgc gctgctcgac ggtctgctgg gcccgctcga
     6841 tggccttggt gaccatcttg gcctcgagcg gcacgtcgtc gggcagggcc ttgcccatga
     6901 cccagttgac ggcgccggtg gcgaagaggc gcatcaggtc gtcctcgagg gacaggtagt
     6961 agcggctctc gccagggtcg ccctggcggc cggcgcgacc acggagctgg tcgtcgatgc
     7021 ggcgggagtc gtggcgctcg gtgccgagca cgtacaggcc tccgagcgcc cggaccttgt
     7081 cgccctcggc ctggcactgc ggctcgtact tggcgatggt cttggccagg cgctcggcgt
     7141 tctcggggtc gtccgcgtcg aggccctcgt tgtgcagctc ctgcttggcc agcagctcgg
     7201 ggttgccacc gaggaggatg tcgacgcctc ggccggccat gttggtggcc acggtgacgg
     7261 tgttgaggcg accggcctgg gcgacgatgt cggcctcccg gaactggcgc tcgggcttgg
     7321 cgttgaggac ctggtgcttc acgccccgct tgtcgagcgt gcggctca
//
LOCUS       DATISU010000037         3626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_48246, whole genome shotgun sequence.
ACCESSION   DATISU010000037 DATISU010000000
VERSION     DATISU010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48246"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1352)
                     /locus_tag="VLR27_01810"
     CDS             complement(180..1352)
                     /locus_tag="VLR27_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02207.1"
                     /translation="MTDGDEVGGRPLRGVVEVEWDGWEDDESRSKKPLVVGALLFLAV
                     LAVAGLTWWAVDASEVMLPGDEAAAPDEPDAPSGPPAPAAPTIDELREAIPAALEGCV
                     PPADRPDGVGPDGTVRLECPRDAVPELVTFTLFPDVEARDQAFRDTVTALELDPAAAG
                     ECALGAGAVHDHRGRRGRGQVACRSVDGRVDIAWTDGTAPVLGVAGGFGRYPVHYGFW
                     SELVGRTDAEFPLPLEQQLLDELPDELTTRCDRDLDLLDRAAGVAAVTCRPATGAAAI
                     VSWVRFAGPDDMTAWLEGEQAALGEAVVDASDDACRPDGRGPSGELPVPWLGATTYRQ
                     AASTGTILCFVDGADRDVLVWSRSGADIGSIAVADDSVDSTMADLAEWWELGGHRP"
     gene            complement(1363..2946)
                     /locus_tag="VLR27_01815"
     CDS             complement(1363..2946)
                     /locus_tag="VLR27_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HSP02208.1"
                     /translation="MTDPVVEAERRRTFAIISHPDAGKTTLTEKFLLYGGAVQDAGQV
                     KARGERRRVRSDWMDMEQQRGISITSTVLQFPYRNRVLNLLDTPGHRDFSEDTYRVLA
                     AADAAIMVLDASSGIEPQTLKLFEVCRERRIPLLTFINKWDRPGKEALELTDEIEETL
                     GLATMPITWPVGIAGDFRGLIDRRSGDYVRFSRTARGATEAGEEILDAAAAEEVDGES
                     YLAAVEQIELLEAVGSDFDQELFEMAEATPVFFGSALTNYGVRLLLDAVADLAPSPQP
                     RPDVDGDARPLASPFSGFAFKVQANMDPSHRDRIAFVRVCSGRFERGMVVTHARTGKP
                     FGTKYAHSVFGQERDTLDEAFPGDVIGLVNATEVRVGDTLYLEPPVEFPGIPAFAPEL
                     FAIARPKDVGRFKQFRKGIAQLDEEGVVQVLRDRDLGDQEPMLAAVGQMQFEVASARL
                     ADEFGSPAEISMTRYSIARVTDEETMANLRGMSGVRVLERSDGTLLALFESVYWLQRI
                     ESDHPDWTLDQLVAEGMAG"
     gene            complement(2943..3332)
                     /locus_tag="VLR27_01820"
     CDS             complement(2943..3332)
                     /locus_tag="VLR27_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02209.1"
                     /translation="MADDLCPPPSYRDGRGRPLLDPRSLWGLELRYLLCDLIASRGGA
                     VCTVAELANELERGGFELGGRPSKEISDALRWEVARGRVVRVSRGRYRTGTIPGATRR
                     RIRKVARHRTEQVGSWRVRQAAGSLDP"
BASE COUNT          515 a         1311 c         1323 g          477 t
ORIGIN      
        1 ccaccagaac gcagtggacg tcgccgaccg gttggccgac ggcgtgctgg tcacccgcga
       61 cggggacggc cacaccatcg tcttccaggg cgatgcgtgc atcgacgcca tcaccatcgc
      121 ctacctggtc gacgaggtcg tgccgcccgc cggcagccgc tgctgagccc gactcccggt
      181 cagggccggt ggccgccgag ctcccaccac tcggcgaggt cggccatggt gctgtcgacg
      241 ctgtcgtcgg ccacggcgat cgacccgatg tccgccccgg accggctcca caccaggacg
      301 tcgcggtccg cgccgtcgac gaagcacagg atcgtccccg tcgaggcggc ttggcggtac
      361 gtcgtcgcac ccaaccacgg cacggggagc tcgccggacg gaccccgacc gtcgggacgg
      421 caggcgtcgt cgctcgcgtc gacgacggcc tcgccgagcg cggcctgctc gccctcgagc
      481 caggccgtca tgtcgtcggg accggcgaag cgcacccacg acacgatggc cgccgcgccg
      541 gtcgcgggcc ggcaggtgac cgcggcgacg ccagcggccc ggtcgagcag gtcgaggtcg
      601 cggtcgcacc gggtcgtgag ctcgtcgggc agctcgtcga ggagctgctg ctcgaggggg
      661 agggggaact cggcgtcggt gcgaccgacg agctcggacc agaacccgta gtgcaccggg
      721 tagcggccga agccgccggc cacgccgagc accggcgcgg tgccgtcggt ccaggcgatg
      781 tcgacccggc cgtccacgct gcggcacgcc acctgcccgc gcccgcgccg gccccgatgg
      841 tcgtggaccg cgccggcccc gagcgcgcac tcccccgccg cagccgggtc cagctcgagg
      901 gcggtgacgg tgtcgcggaa cgcctggtcc cgggcctcga cgtcggggaa gagcgtgaac
      961 gtcacgagct cgggcaccgc gtcacggggg cactcgagcc ggacggtgcc gtcggggccg
     1021 acgccgtcgg gccgatcggc gggtggcacg cagccctcca gcgccgccgg gatcgcctcg
     1081 cgcagctcgt cgatggtcgg cgctgcgggg gcgggtgggc cgctcggggc atcgggctca
     1141 tccggcgccg ctgcctcgtc gcccgggagc atcacctcgc tggcatccac ggcccaccag
     1201 gtgaggccgg cgacggccag caccgcgagg aagaggagcg caccgaccac gagcggcttc
     1261 ttcgagcggg actcgtcgtc ctcccagccg tcccactcga cctcgaccac gccccggagg
     1321 ggccggccac ccacctcgtc gccgtcggtc atcgggtact tcctaccccg ccatgccctc
     1381 ggcgacgagc tggtcgaggg tccaatccgg gtgatcggac tcgatgcgct gcaaccagta
     1441 cacggactcg aacagcgcga gcagcgtgcc atcggagcgc tcgagcaccc gcacgccgct
     1501 catcccccgc aggttcgcca tggtctcctc gtcggtgacc cgggcgatcg agtagcgggt
     1561 catggagatc tcggcgggcg agccgaactc gtcggcgagc cgggccgacg cgacctcgaa
     1621 ctgcatctgg ccgacggcgg cgagcatggg ctcctggtcg ccgaggtcgc ggtcgcgcag
     1681 cacctgcacc acgccctcct cgtcgagctg ggcgatgccc ttgcggaact gcttgaagcg
     1741 gccgacgtcc ttggggcggg cgatggcgaa cagctcgggg gcgaaggccg ggatgccggg
     1801 gaactccacg ggtggttcga ggtagagcgt gtcgcccacc cgcacctcgg tggcgttcac
     1861 caggccgatc acgtcgccgg ggaaggcctc gtcgagcgtg tcgcgctcct ggccgaacac
     1921 cgagtgggcg tacttggtgc cgaagggctt gccggtgcgg gcgtgggtga ccaccatccc
     1981 ccgctcgaac cggccggagc acacccgcac gaaggcgatg cggtcgcggt ggctcgggtc
     2041 catgttggcc tggaccttga aggcgaagcc ggagaagggc gacgcgagcg ggcgggcgtc
     2101 gccgtcgacg tcggggcggg gctgcggcga cggggccagg tcggcgacgg cgtcgagcag
     2161 gagccgcacg ccgtagttgg tgagcgctga cccgaagaag accggggtgg cctcggccat
     2221 ctcgaagagc tcctggtcga agtcggaccc caccgcctcc agcagctcga tctgctcgac
     2281 ggcggccagg tagctctcgc cgtcgacctc ctcggcggcc gcggcatcga ggatctcctc
     2341 gccggcctcg gtcgcacccc gggccgtgcg ggagaagcgc acgtagtcgc cgctgcggcg
     2401 gtcgatgagg ccgcggaagt cgccggcgat gcccaccggc caggtgatcg gcatggtggc
     2461 caggccgagg gtctcctcga tctcgtcggt gagctcgagg gcctccttgc cgggccggtc
     2521 ccacttgttg atgaaggtga gcagcgggat gcgccgctcc cggcacacct cgaacagctt
     2581 cagggtctgg ggctcgatgc ccgagctggc gtccaggacc atgatcgcgg cgtcggcggc
     2641 ggccagcacg cggtaggtgt cctccgagaa gtcgcggtgg cccggggtgt cgagcaggtt
     2701 cagcacccgg ttccggtacg ggaactgcag cacggtcgag gtgatggaga tgccgcgctg
     2761 ctgctccatg tccatccagt cggaccgcac ccgccggcgc tcgccacggg ccttgacctg
     2821 gccggcgtcc tgcaccgccc cgccgtagag caggaacttc tcggtgagcg tcgtcttgcc
     2881 cgcgtcggga tggctgatga tggcgaacgt gcggcggcgt tcggcctcga cgacgggatc
     2941 ggtcacgggt caagtgaacc ggccgcctgc cggacccgcc aactcccgac ctgctcggtc
     3001 cggtgcctcg ccaccttgcg gatgcgtcgg cgcgtcgctc cggggatcgt gcccgttcgg
     3061 taccggccgc ggctcacccg cacgacccga cctcgtgcca cctcccaacg gagcgcatcg
     3121 gagatctcct tgctcggccg gccaccgagc tcgaatccac cccgctcgag ctcgttcgcg
     3181 agctccgcca cggtgcagac ggcgccgccc cgcgaggcga tgagatcgca gagcaggtag
     3241 cggagctcga ggccccacag cgaccgggga tccagcagcg ggcggccgcg accatcccga
     3301 tacgacggtg gaggacacag gtcgtcggcc atgacgtcca tgcttccgag ccccaccgac
     3361 aggaacgccg gcgaccccgg cgctgatccg tcgagccgga gtcggcccat caccgttgcg
     3421 ccacgtcgca gcgacaaccc gaccagggcg tccccacagc ggtggtggtt cgcgacgacg
     3481 ttcagaacgc cagatctgtg ccgacacacc gaccggtcga tgccaggtgg cagacgggat
     3541 ccggctgcag aacgccagcg ggcggtggcc gcaagagggc gtttgcgcac ggggtcggca
     3601 gaggcgcagg gcacggtgtc cggccc
//
LOCUS       DATISU010000038         1636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_700518, whole genome shotgun sequence.
ACCESSION   DATISU010000038 DATISU010000000
VERSION     DATISU010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700518"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VLR27_01825"
     CDS             <1..393
                     /locus_tag="VLR27_01825"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HSP02210.1"
                     /translation="PMLEAGLMGFLGIRHTGCGAGWIEAELDVRADLLTPFGTAHGGV
                     VSALADHVLGSVMYPVMASGQWAATTEFKLNLLRPVREGVLRARADIISMSRRTAVVR
                     IDITNDTTEGGARLAAAAQGTVSIQDPR"
     gene            409..1431
                     /locus_tag="VLR27_01830"
     CDS             409..1431
                     /locus_tag="VLR27_01830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HSP02211.1"
                     /translation="MELHVMAGGRRLDKVQEFARSVDEAGLDGIVYVESGRTAYLSVA
                     AAALAAPRLQLSTGIAVAFPRSPTVTATNAWELQDITDGKFRLGLGTQVRAHIERRYG
                     VDFDPPGPRMREYVQAVKALFASFRGDSLEFDGEYHELSLMPQMWSPGPIDHPDPPVD
                     IAAVGPWMVQMAGAVADGVHVHPFHSRTYLDDHLLPGLREGADDAGRTLDDLTLSVPV
                     FTVVGDTEEERARWRDRARFQIAFYGSTKNYAFMFDMLGFEGTSSAINDKLKAGDMPG
                     MAALITDEMLQHFAVEAAWDDLADVLRTKYDGIADRLVLYFAEEMHQGDPRSLARMGE
                     VAAALH"
BASE COUNT          236 a          621 c          546 g          233 t
ORIGIN      
        1 ccgatgctcg aggccgggct catgggcttc ctcggcatcc ggcacaccgg gtgcggcgcc
       61 gggtggatcg aagccgagct cgacgtgcgc gccgacctgc tcaccccgtt cggcaccgcc
      121 cacggcggcg tcgtgtccgc gctggccgac cacgtcctgg gctcggtgat gtatccggtc
      181 atggcgtcgg gacagtgggc ggccaccacc gagttcaagc tcaacctcct gcgccccgtg
      241 cgcgagggcg tgctccgggc ccgggccgac atcatctcga tgagccggcg cacggccgtc
      301 gtgcgcatcg acatcaccaa cgacaccacc gaaggtggcg cccgcctcgc agctgctgcg
      361 cagggcaccg tctcgatcca ggatccccgg taggggagag gagcacccat ggagctgcac
      421 gtgatggccg gcggacgccg gctggacaag gtccaggagt tcgcccgctc cgtcgacgag
      481 gccggcctcg acggcatcgt ctacgtcgag tcgggccgca ccgcctacct gtcggtggcc
      541 gcggccgcgc tcgccgctcc gcgcctgcag ctctccaccg gcatcgcggt ggcgttcccc
      601 cgcagcccga ccgtgacggc gacgaacgcc tgggagctgc aggacatcac cgacgggaag
      661 ttccgcctcg gcctgggcac gcaggtgcgg gcccacatcg agcgccggta cggcgtggac
      721 ttcgacccgc ccggcccccg catgcgcgag tacgtgcagg ccgtcaaggc gctgttcgcg
      781 tcgttccgcg gcgacagcct cgagttcgac ggtgagtacc acgagctctc gctcatgccc
      841 cagatgtggt cgcccggtcc catcgaccac cccgaccccc cggtcgacat cgccgcggtg
      901 ggcccgtgga tggtccagat ggccggcgcg gtggccgacg gcgtgcacgt gcaccccttc
      961 cactcccgca cctacctcga cgaccacctg ctgcccggcc tgcgcgaggg cgccgacgac
     1021 gctgggcgca ccctcgacga cctgaccctc tccgtgccgg tgttcaccgt cgtcggcgac
     1081 accgaggagg agcgggcccg ctggcgtgac cgggcgaggt tccagatcgc gttctacggg
     1141 tcgaccaaga actacgcgtt catgttcgac atgctcggct tcgagggcac gtcctcggcc
     1201 atcaacgaca agctcaaggc cggcgacatg ccgggcatgg ccgccctcat caccgacgag
     1261 atgctccagc acttcgcggt cgaggccgcc tgggacgacc tggccgacgt gctgcgcacc
     1321 aagtacgacg gcatcgccga ccgcctggtc ctgtacttcg ccgaggagat gcaccagggc
     1381 gatccgcggt cgctggcccg catgggtgaa gtcgccgccg cgctgcacta ggccgggatt
     1441 gcccctcggg ccctccggcc tgtgagcatt ggggggtggc ccccgaagac aacaccgcga
     1501 ccttcgccga cctcggcctg cgcgacgaga tcgtcgcccg cctggtcgag ctcgactacg
     1561 aagaacccac cccgatccag cgcgaggcca tccccgtgct cctcgagggc tcggacctgc
     1621 tcggccaggc cgcgac
//
LOCUS       DATISU010000039        10815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_141579, whole genome shotgun sequence.
ACCESSION   DATISU010000039 DATISU010000000
VERSION     DATISU010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 10815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10815
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141579"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..906)
                     /locus_tag="VLR27_01835"
     CDS             complement(121..906)
                     /locus_tag="VLR27_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HSP02212.1"
                     /translation="MALNVVVCVKQIPDPADPGALDADFTLKRDVKLILDESDSYGVE
                     MALQLVDTAGGGEVTLVSMAPNGEVSGLRTALAMGAAKGVLVSDDALAGSGALETAKT
                     LAAAIGKAGDYDLVISGVESSDGYTGVVPQMLAAQLGHPMVSFAKSVEVSDGKVKVAR
                     QTETGSQDVEAPTPCVVSVTAGVVEPRYPSFKGIMAAKSKPVDTLALGDLGIDGGNVG
                     WAGAGQEITAVEDAPARQAGEIVEDDGEGFQKIVAKLEELKVI"
     gene            962..1894
                     /locus_tag="VLR27_01840"
     CDS             962..1894
                     /locus_tag="VLR27_01840"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HSP02213.1"
                     /translation="MRVLLIVNAFASSVTARSMVVIRKAFSADHDVEMVETSRRGHAA
                     RLARGAANDGVDVVVVMGGDGTLNEAANGLVGTDTALGVLPGGSTNVFARTIGLPNDP
                     VEATGVLLDALAKGSIRRVGLGAVNGRCFLFHTGLGFDAAVVEQVERRSSLKRYAGHP
                     LFVYAGFATWFRHFDTSRPRLAVRLPDGRTVDDGYMAIVLNTSPYTYLGNVPLDLAPE
                     ATLDTGLSVVVVRTMQFARVLGLIGSALASGRHIRSSRYALLAHDLDELEVTGHGPLP
                     YQVDGDFLGNGETFRFEHRADALDLVLPAGPAGA"
     gene            complement(1895..2602)
                     /locus_tag="VLR27_01845"
     CDS             complement(1895..2602)
                     /locus_tag="VLR27_01845"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HSP02214.1"
                     /translation="MFTPGANATPLVYGHRGASAEAPENTLEAFALARTQGAHGVELD
                     VRRCRDGDMALHHDAELADGRRIVELVRADLPAGSCTLAEALDVCEGMIVNIEIKNVS
                     IDPDHDPECALADTVVALLHERGGRDRVIVSSFGLPTIDRVRAIDSDVPTAFLTFIEP
                     MGPKAIDLAARHGHQAIHPHVATVDEAYVALAHEHGLAVNVWTVDDPDQIRQLAAWGV
                     DGIVTNVPAIAIDALRG"
     gene            2772..3068
                     /locus_tag="VLR27_01850"
     CDS             2772..3068
                     /locus_tag="VLR27_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HSP02215.1"
                     /translation="MTTTRSIPLTLERDEWRQFAECRDTDPDLFFPVGTTGPALEQIA
                     NAKAVCRTCDVQGLCLEYAVSTNQDSGVWGGTSEEERRQMRRARQRLEKQQRSA"
     gene            complement(3156..4634)
                     /locus_tag="VLR27_01855"
     CDS             complement(3156..4634)
                     /locus_tag="VLR27_01855"
                     /inference="COORDINATES: protein motif:HMM:NF023702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HSP02216.1"
                     /translation="MASLVELARTLTPLDGPQAQQLQRLAASWGLLADFCFSDLLLFA
                     PVGDFDGGEFVVLAQVRPTTGQTVYRGDWVGTRLDEDERPLVARSYRNGEIIEGEIHL
                     SVVRERVRVLCVPVRREGKVIGVLTREAAPSIGRQHGELERTYLDIFNRFARMIASGD
                     FPYEDKDDTAEAPRVGDGVLLVDPAGRVEYASPNAVSAFHRVGVHANAEGLRFGELGL
                     DESAVRLAFHKRQPTVVEVERGSEITVVFRCLPITDRGEVSGAVVLVRDISELRRRDR
                     LLLSKDATIREIHHRVKNNLQTISSLLRLQGRRLDSAEAKAAIEESARRIRSIAVVHE
                     ILSREAGDDVPFVEIARELSRMVDESLMNPDRPFRVRFSGEAGTIPAAPATSLSVVLN
                     ELLQNAVDHAFPERVDLAEEPGYVDVLLEREEDQLTVRVTDNGVGIPEGFDLDASTGL
                     GLSIVRTLVETELEGEITMQWVHESGPRHGTEVQVVVSTKHF"
     gene            complement(4654..5820)
                     /locus_tag="VLR27_01860"
     CDS             complement(4654..5820)
                     /locus_tag="VLR27_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HSP02217.1"
                     /translation="MARTSRRAAGTLEQLLAAKEIVICCGSGGVGKTTTAAAAGAMAA
                     AQLGGKVLVVTVDPARRLANALGLESFGNVETRVPDSAFAEAGVECQGELWAAMLDTK
                     QSWDDLVRQHAPDAATRDAILANPLYTNITGRFVQSHDYIAMERLYEIHASGRYDLVI
                     VDTPPTRNALDFLEAPERMADFFSSRLLRWLTVPYRSRFVNMATKPFYKVADTILGSA
                     FLEDIAEFFLLFQTMYDGFVQRARAVQRILEDKRTTFLVVSTLESAPLREAEYFMSAL
                     EERRFHLGAVVLNKVLPSYLLDAGAADAASRLGADPAAAAAALDELGDPEAVARVVAE
                     ISQSFLNFRVVASREAEQRKELGRTPDVLATVPYFDSDIYDLAGLVRVGEQIWR"
     gene            complement(5820..6767)
                     /locus_tag="VLR27_01865"
     CDS             complement(5820..6767)
                     /locus_tag="VLR27_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HSP02218.1"
                     /translation="MIPLLDRKLLFVTGKGGVGKTTIAAALGTLAADHGKRTLVCEVD
                     AKGNLSDFFEAGRTGFDPTPISENLFAMSMNTEESLKEYLSLQLKIPVIARIGPLART
                     FDFVATAAPGVKEILTVGKLAWEVREQHYDLVVVDASASGHIVGQLAAPDAINELVQV
                     GLVRDQTGWMLDILGDPAQTGVVIVAAPEEMPVQETIELAGRLREETVVELAAVIVNR
                     VLPELFNAGEEALFERLRSDEALVATLAEAGGGPVEPILDAAELAVSLRRSRAVHLQK
                     LRTELAGTEMLYVPYLFQRSHGVRATNRIAGHLGEELGF"
     gene            complement(6784..7017)
                     /locus_tag="VLR27_01870"
     CDS             complement(6784..7017)
                     /locus_tag="VLR27_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HSP02219.1"
                     /translation="MDVRIGVTYTAKEIDLELADDADPAQLQADVDAAIAGDHLVLWL
                     TDRKGRRVGVPTEKIAYVEIGTPEAARRIGFGG"
     gene            7084..7746
                     /locus_tag="VLR27_01875"
     CDS             7084..7746
                     /locus_tag="VLR27_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSP02220.1"
                     /translation="MTALGPEALEFVRDRVIHVVGHELRTPLTTVRGLAELLVDASDE
                     ELHETLIPALIRNARRAERLLDDLLIAAEIHTARPTDPATPVDLGALVAESTADTQVT
                     VEGAPAGPVLAHHDAVGHALDHLVDNAEQYGDGPSTVRFEDDEESVTVVVETPVDHEL
                     ADPQLSFELFFRGEQAVTRAAGLGIGLPVARALAQMDGGDLTIDQDGGAVLARLSLPR
                     SR"
     gene            7743..8159
                     /locus_tag="VLR27_01880"
     CDS             7743..8159
                     /locus_tag="VLR27_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP02221.1"
                     /translation="MSQAPLSDPPEQIDVVIADDEVDIRLLLKLQLRQAGINVVGEAA
                     DGIEAVEQCESLRPHVVVLDLLMPRLSGFDAIPQLQERSPEVRIVAYSAVAGEFARTE
                     MARHGIPLRLKNGDAEPLVRTIRELAGHPADEHEVR"
     gene            complement(8163..9302)
                     /locus_tag="VLR27_01885"
     CDS             complement(8163..9302)
                     /locus_tag="VLR27_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HSP02222.1"
                     /translation="MPTEAQIIEALRPVEDPELHRSIVDLGMVRFVRIDGQSVQVEIA
                     LTVPGCPLKNEIQRRVGEAVHAFDDVQSVDVTFDVMSDEERARVRELMVGDPKATAGS
                     QAAHGHAEGRTIPFSEPGSKTRVLLVASGKGGVGKSSVTTNLAVSLAKRGNKTAIVDA
                     DVWGFSIPRMLGIDRAPVVIDSMLIPPESHGVSCISMGFFAQEDQAVIWRGPMLHKAL
                     EQFLTDVYWDDPDFLVVDLPPGTGDISISLAQFLPRAEVYVVTTPQPAAQRVAQRAAF
                     MAEKVNLEVKGVIENMSWFTGDDGTRYEIFGSGGGEELATRLDKPLLGKVPLVPELRE
                     GSDTGMPIVVSSPESEAAGAFDEIARAIVEDLAPTKRFHPELKIT"
     gene            9374..10105
                     /locus_tag="VLR27_01890"
     CDS             9374..10105
                     /locus_tag="VLR27_01890"
                     /inference="COORDINATES: protein motif:HMM:NF037269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP02223.1"
                     /translation="MDADSPTPLSAADFSSAVTAITSAFGDPTRREIYLWLRDQPEGV
                     TASAVAERFELHSNVARHHLDKLAGGGYVEVSTARLAGSGAGRPSKRYKAIGNENPIE
                     FAVRHDDLLVTLLGKSLALLPGDVAARMAEEVGVEYGRSLAASMGSGEHVQRSFRSAL
                     HAVADALTSHGFAAHAEKQGDELRIVAQHCPFGDAAIEHPVICAVDRGLVKGMLSSLY
                     GDTAPATETTQALGSETCVTVVTSR"
     gene            10135..>10815
                     /locus_tag="VLR27_01895"
     CDS             10135..>10815
                     /locus_tag="VLR27_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSP02224.1"
                     /translation="MSSPDLGHYLDHASTSPLRPEARAAMIEALGAPLADPSRIHEPG
                     MTARVALEQAREVVAGFLGARPREVVFTSGASESIAAACWGAAPRGRTQVLSAVEHSA
                     VRLAAEQHGPTAVVGCDRTGRVDPDELLAAVDDDTALVHLQLGNHEVGTTQPVAEVVA
                     ACRERGVLVHVDAAQAAGRVPFEFGALGADLVSVSGHKLGGPAGTGALLVRRGLRIRP
                     LIVGGEQER"
BASE COUNT         1532 a         3825 c         3860 g         1598 t
ORIGIN      
        1 gccttggtga gcagctcctt gctgagcgtc gtcagcgagc cgttctcgtc ggtctcgacg
       61 aagacccaca ccttggaaag agccatgtcg ttcttccttc tctcgttctg ggtcgtccgc
      121 tcagatgacc ttgagctctt cgagcttggc cacgatcttc tggaagccct caccgtcgtc
      181 ctcgacgatc tcgccggcct ggcgagcggg ggcgtcctcc acagcggtga tctcctgccc
      241 ggcgccggcc cagccgacgt tgccgccgtc gatgccgagg tcgccgaggg cgagggtgtc
      301 gaccggcttg gacttggctg ccatgatgcc cttgaaggac gggtagcggg gctcgaccac
      361 gccggcggtc accgagacca cgcagggggt gggagcttcg acgtcctggg agccggtctc
      421 ggtctggcgg gccaccttga ccttgccgtc ggacacctcg accgacttgg cgaaggacac
      481 catggggtgg ccgagctggg cggcgagcat ctggggcacg acgccggtgt agccgtcgga
      541 ggactcgacg cccgagatca cgaggtcgta gtcaccggcc ttgccgatgg ccgcagccag
      601 ggtcttggcc gtctcgaggg cgccggaacc ggcgagggcg tcgtcggaga cgagcacgcc
      661 cttggcggca cccatggcca gcgccgtgcg cagacccgag acctcaccgt tcggggccat
      721 cgagaccagg gtgacctcac caccgccggc cgtgtcgacc agctggagcg ccatctcgac
      781 cccataggag tcggactcgt ccaggatgag cttgacatcg cgcttgaggg tgaagtcagc
      841 gtccagagcg ccgggatcgg cgggatccgg gatctgcttc acgcagacga ccacgttgag
      901 agccatgtgc ggagtcaagc atcaggcccc gtcgttggtc gaattgcact aggacagggg
      961 cgtgcgagtc ctgctcatcg tgaacgcgtt cgcctcgtcg gtcaccgccc gctcgatggt
     1021 cgtcatccgc aaggcgttct cggccgacca cgacgtcgag atggtggaga cgagccgccg
     1081 cggccacgcg gcgcgcctgg cccggggcgc cgccaacgac ggcgtcgacg tcgtggtggt
     1141 catgggcggc gacggcacgc tcaacgaggc ggccaacggc ctggtgggca ccgacaccgc
     1201 cctcggcgtg ctgcccggcg gctccaccaa cgtgttcgcc cgcaccatcg ggctgcccaa
     1261 cgacccggtc gaggccaccg gggtgctgct cgacgcgctg gccaagggct ccatccgccg
     1321 cgtcgggctc ggcgcggtca acggccggtg cttcctgttc cacaccggcc tcggcttcga
     1381 cgccgccgtc gtcgagcagg tggagcgccg ctcgtccctc aagcgctacg ccggacaccc
     1441 gctcttcgtc tacgccggct tcgcgacctg gttccgccac ttcgacacct cccgcccgcg
     1501 cctcgcggtg cgcctccccg acggccgcac ggtcgacgac ggctacatgg cgatcgtgct
     1561 caacacgagc ccgtacacct acctcggcaa cgtgcccctc gacctggctc ccgaggccac
     1621 cctcgacacc gggctctccg tcgtcgtcgt gcgcacgatg cagttcgccc gggtgctggg
     1681 gctgatcggc tccgcgctcg cgtcgggccg ccacatccgc tcatcccgct acgccctgct
     1741 cgcccacgac ctcgacgagc tcgaggtcac cggccacggc ccgctcccct accaggtgga
     1801 cggcgacttc ctcggaaacg gggagacgtt ccgcttcgag caccgcgccg acgccctcga
     1861 cctcgtgctg cccgccggtc ccgccggcgc gtgatcagcc ccggagcgcg tcgatggcga
     1921 tcgccggcac gttcgtcacg atgccgtcga cgccccacgc cgccagctgg cggatctggt
     1981 cggggtcgtc caccgtccag acgttgacgg ccagcccgtg ctcgtgggcg agcgcgacgt
     2041 aggcctcgtc caccgtcgcc acgtgcgggt ggatcgcctg gtggccgtgg cgggcggcca
     2101 gatcgatggc cttgggtccc atgggctcga tgaaggtcag gaacgcggtg ggcacgtcgg
     2161 agtcgatggc ccggacccgg tcgatggtgg gcaggccgaa gctcgacacg atcacccggt
     2221 cacgcccgcc gcgctcgtgc aggagggcga cgacggtgtc ggccagcgcg cactccgggt
     2281 cgtggtcggg atcgatggag acgttcttga tctcgatgtt gacgatcatc ccctcgcaca
     2341 cgtcgagggc ctcggccagg gtgcacgagc ccgccggcag gtcggcccgg accagctcga
     2401 cgatgcggcg gccgtcggcc agctcggcat cgtggtggag cgccatgtcg ccgtcccggc
     2461 accggcgcac atcgagctcg acgccgtgcg cgccctgcgt ccgggcgagg gcgaaggcct
     2521 ccagcgtgtt ctcgggtgcc tcggccgacg caccgcggtg gccgtacacg agaggggtgg
     2581 cgttcgcgcc gggagtgaac aacgtctcgg gaaacctccg aagaaaccct tgtgacgggc
     2641 gtgtttcgac gttactttgg tcgacggccc tgcgagacct cagttcgtcc gcagaggcat
     2701 gtgaacgccg tcacaaatcg ggtcctctgc accgccagca cgacccctcg acccttggag
     2761 gccccgtggc cctgaccacg acgcgctcga tccccctgac gctcgaacga gacgagtggc
     2821 gccagttcgc ggagtgccgc gacaccgatc ccgacctgtt cttcccggtc ggcaccacgg
     2881 gccccgccct cgagcagatc gccaacgcca aggccgtctg ccgcacctgc gacgtccagg
     2941 gcctctgcct cgagtacgcc gtgtccacca accaggacag cggtgtctgg ggtggcacct
     3001 ccgaggagga gcgccgccag atgcgtcggg cccgccagcg cctcgagaag cagcagcgca
     3061 gcgcgtagcc caccgatccc gtgccggcgt tccggcagtg acacgacctc ccctcgggga
     3121 ggtcgttgcg cgtccggcca gggagcggag cggtcctaga agtgcttggt ggacacgacc
     3181 acctggacct cggtgccgtg gcgggggccg gactcgtgga cccactgcat ggtgatctcg
     3241 ccctcgagct cggtctcgac gagcgtgcgc acgatcgaca gcccgagtcc ggtggaggcg
     3301 tcgaggtcga acccttcggg gatgcccacg ccgttgtcgg tgacccgcac ggtcagctgg
     3361 tcctcctccc gctcgagcag cacgtcgacg tagccgggct cctcggccag gtcgacccgc
     3421 tccgggaagg cgtgatcgac cgcgttctgc aacagctcgt tcagcaccac cgacagcgac
     3481 gtggccgggg cggccgggat cgtgcccgcc tcacccgaga agcgcacccg gaacggccgg
     3541 tccgggttca tcaggctctc gtccaccatg cgggacagct cgcgggcgat ctccacgaac
     3601 gggacgtcgt cgccggcctc gcgggacagg atctcgtgga cgacggcgat cgagcggatg
     3661 cgccgggcgg actcctcgat cgcggccttg gcctcggcgg agtcgagccg acgtccctgc
     3721 aaccgcagca gcgaggagat cgtctgcagg ttgttcttca cccggtggtg gatctcccgg
     3781 atggtggcgt ccttggagag caggagccgg tcccggcggc ggagctcgga gatgtcccgc
     3841 acgagcacga ccgcaccgga cacctccccc cggtcggtga tcggcaggca gcggaacacc
     3901 acggtgatct cgctgccccg ctccacctcg acgaccgtcg gctggcgctt gtggaacgcc
     3961 aggcgcaccg cgctctcgtc gaggccgagc tcgccgaagc gcaacccctc ggcgttggca
     4021 tggacaccga cgcggtggaa cgccgacacc gcgttcggtg acgcgtactc gacccgaccg
     4081 gcggggtcga cgagcagcac gccgtcgccc acccggggcg cctcggcggt gtcgtccttg
     4141 tcctcgtagg ggaagtcgcc cgacgcgatc atgcgggcga agcggttgaa gatgtcgagg
     4201 taggtgcgct cgagctcgcc gtgctggcgc ccgatggacg gcgccgcctc ccgggtgagg
     4261 acgccgatga ccttgccctc gcgccggacc ggcacgcaca gcacccggac ccgctcccgc
     4321 acgaccgaca ggtggatctc gccttcgatg atctcgccgt tgcggtagga gcgtgccacc
     4381 agcgggcgct cgtcctcgtc gagccgggtg ccgacccagt cgccgcggta gacggtctgg
     4441 ccggtggtgg gccgcacctg ggcgagcacc acgaactcgc cgccgtcgaa gtcgccgacc
     4501 ggggcgaaga ggagcaggtc ggagaagcag aagtcggcga gcaggcccca cgaggcggcg
     4561 aggcgctgca gctgctgggc ctgggggccg tcgagcggcg tgagcgtgcg ggccagctcg
     4621 accagggagg ccatcagcgg tcctcggggg cggtcatcgc cagatctgct cgccgacgcg
     4681 gacgaggccg gcgaggtcgt agatgtcact gtcgaagtac ggcacggtgg cgagcacgtc
     4741 cggggtccgc cccagctcct tgcgctgctc ggcctcccgg ctggcgacga cgcggaagtt
     4801 caggaagctc tggctgatct cggccacgac ccgggccacg gcctcggggt cgccgagttc
     4861 gtcgagcgcg gctgcggcgg cggccgggtc ggcgccgagg cggctggcgg cgtcggcggc
     4921 gccggcatcg agcaggtagg acggcagcac cttgttgagc accacggccc cgaggtggaa
     4981 gcgccgctcc tcgagggccg acatgaagta ctcggcctcg cgcagcggag ccgattcgag
     5041 ggtgctcacc accaggaacg tcgtgcgctt gtcctcgagg atgcgctgga cggcgcgggc
     5101 ccgctgcacg aagccgtcgt acatggtctg gaacaggagg aagaactcgg cgatgtcctc
     5161 gaggaacgcc gacccgagga tcgtgtcggc caccttgtag aagggcttgg tcgccatgtt
     5221 cacgaaccgg gaccggtacg gcacggtgag ccagcgcagg aggcgggacg agaagaagtc
     5281 cgccatccgc tcgggtgcct cgaggaagtc gagggcgttg cgggtgggcg gcgtgtccac
     5341 gatgaccagg tcgtagcgac ccgaggcgtg gatctcgtag agccgctcca tggcgatgta
     5401 gtcgtggctc tgcacgaagc ggccggtgat gttggtgtag agcgggttgg ccaggatcgc
     5461 gtcgcgggtg gcggcgtcgg gtgcgtgctg gcgcaccagg tcgtcccagg actgcttggt
     5521 gtcgagcatg gccgcccaca gctcgccttg gcactcgacg ccggcttcgg cgaaggccga
     5581 gtccgggacc cgggtctcga cgttgccgaa ggactcgagg ccgagggcgt tggccagccg
     5641 gcgagccggg tcgacggtga cgacgaggac cttgccgccg agctgggctg cggccatggc
     5701 cccggccgcg gccgcggtgg tggtcttgcc caccccgccg gacccgcagc agatgacgat
     5761 ctccttggcg gcgagcagct gctcgagcgt gccggcggcg cggcgtgacg tgcgagccat
     5821 cagaagccca gctcctcgcc gaggtgcccg gcgatgcggt tggtggcccg gacgccgtgg
     5881 ctccgctgga acaggtacgg cacgtagagc atctcggtgc cggccagctc ggtgcgcagc
     5941 ttctgcaggt gcaccgcccg ggagcggcgc aggctcaccg ccagctccgc ggcgtcgagg
     6001 atcggctcca ccggtccgcc gccggcctcg gcgagcgtgg ccacgagggc ctcgtcgctc
     6061 cgcagccgct cgaacagggc ctcctcgccg gcgttgaaca gctcggggag cacccggttc
     6121 acgatgacgg cggccagctc caccaccgtc tcctcgcgca ggcgcccggc gagctcgatg
     6181 gtctcctgca ccggcatctc ctcgggcgcg gcgacgatga ccacgccggt ctgggccgga
     6241 tcgccgagga tgtcgagcat ccaccccgtc tggtcccgca ccaggccgac ctgcaccagc
     6301 tcgttgatgg cgtcgggggc ggcgagctga ccgacgatgt ggccgctggc cgacgcatcg
     6361 acgaccacga ggtcgtagtg ctgctcgcgc acctcccagg ccagcttgcc caccgtgagg
     6421 atctccttca ccccgggcgc cgccgtggcc acgaagtcga aggtccgggc cagcgggccg
     6481 atccgggcga tgaccgggat cttcagctgc aggctcaggt actccttgag cgactcctcg
     6541 gtgttcatcg acatcgcgaa gaggttctcg gagatggggg tcgggtcgaa cccggtgcgg
     6601 ccggcctcga agaagtccga caggttcccc ttcgcgtcga cctcgcacac cagggtgcgc
     6661 ttcccgtggt cggcggcgag cgtgccgagc gcagcggcga tggtggtctt gccgaccccg
     6721 cccttgccgg tgacgaacag gagctttcgg tcgagcagcg ggatcatggg gtgacccggt
     6781 ggatcagccc ccgaagccga tgcggcgggc cgcctcgggg gtgccgatct ccacgtaggc
     6841 gatcttctcc gtcggcacgc cgacccgacg tcccttgcga tcggtcagcc acagcaccag
     6901 gtggtcgccg gcgatggcgg cgtcgacgtc ggcctgcagc tgggcagggt cggcgtcgtc
     6961 tgccagctcg aggtcgatct ccttggccgt gtaggtcaca ccgatccgga cgtccacttc
     7021 gcacacctcg tgtcgactgc ggttggggcc gccaacctat ccggtgggta cgcttcgtcc
     7081 gccatgaccg cactcggacc cgaggccctg gagttcgtcc gcgaccgggt gatccacgtc
     7141 gtgggccacg agctccggac accgctcacc acggtgcgcg ggttggccga gctgctggtc
     7201 gatgcctccg acgaggagct gcacgagacg ctgatccccg cgctcatccg caacgcccgc
     7261 cgggccgagc gactgctcga cgacctgttg atcgccgccg agatccacac ggccaggccg
     7321 accgacccgg ccacgcccgt cgacctcggg gccctggtcg ccgagtcgac cgccgacacc
     7381 caggtcactg tcgagggcgc acccgccggc ccggtcctcg cccaccacga tgcggtcggc
     7441 cacgccctcg accacctcgt cgacaacgcc gagcagtacg gcgacggccc ctccacggtg
     7501 cgcttcgagg acgacgagga gtcggtgacc gtcgtggtcg agacgccggt cgatcacgag
     7561 ctcgccgacc cgcagctcag cttcgagctg ttcttccgtg gcgagcaggc ggtcaccaga
     7621 gcagcgggac tcgggatcgg gctccccgtc gcccgggccc tcgcccagat ggacggcggc
     7681 gacctcacca tcgaccagga cggcggtgcg gtcctggccc gcctgtcgct cccccgctcc
     7741 cgatgagcca ggcaccgctg agcgatccgc cagagcagat cgacgtcgtc atcgccgacg
     7801 acgaggtcga catccggctg ctgctcaagc tgcagctgcg ccaggcgggc atcaacgtcg
     7861 tcggcgaggc agccgacggg atcgaagccg tcgagcagtg cgaatccctg cgaccccacg
     7921 tcgtggtcct cgacctgctg atgccccggc tgagcgggtt cgacgcgatc ccgcagctgc
     7981 aggagcgcag ccccgaggtg cgcatcgtgg cgtactcggc cgtcgccggc gagttcgccc
     8041 gcacggagat ggcccgccac ggcatccccc tgcgcctcaa gaacggcgac gcggagccgc
     8101 tggtgcgcac gatccgcgag ctggccggcc acccggccga cgagcacgag gtccgctgaa
     8161 cctcaggtga tcttcagctc ggggtggaac cgcttggtcg gggccaggtc ctcgacgatg
     8221 gcgcgggcga tctcgtcgaa cgcgccggcg gcctcggact cgggcgagct cacgacgatc
     8281 ggcatgccgg tgtcggagcc ctcgcgcagc tcggggacca acggcacctt gccgagcagc
     8341 ggcttgtcga gacgggtcgc gagctcctcg cccccgcccg agccgaagat ctcgtagcgc
     8401 gtgccgtcgt caccggtgaa ccaggacatg ttctcgatga cgcccttgac ctcgaggttg
     8461 accttctcgg ccatgaacgc ggctcgctgg gcgacccgct gggcggccgg ctggggcgtg
     8521 gtcacgacgt agacctcggc ccggggcagg aactgggcca gcgagatcga gatgtcgccg
     8581 gtgcccgggg gcaggtcgac gacgaggaag tcggggtcgt cccagtagac gtcggtgagg
     8641 aactgctcga gtgccttgtg cagcatcggg ccacgccaga tgacggcctg gtcctcctgg
     8701 gcgaagaagc ccatggagat gcaggacacg ccgtgggact ccggcgggat cagcatggag
     8761 tcgatgacga ccggggcccg gtcgatgccg agcatccgcg ggatcgagaa gccccagacg
     8821 tcggcgtcga cgatggcggt cttgttgccc cgcttggcca ggctgacggc gaggttcgtc
     8881 gtgaccgagg acttgccgac gccgcccttg ccggaggcga ccagcaggac ccgggtcttg
     8941 gagccgggct cggagaacgg gatggtgcgg ccctcggcgt ggccgtgggc ggcctggctg
     9001 ccggcggtgg ccttgggatc gccgaccatg agctcgcgca cgcgggcgcg ctcctcgtcg
     9061 gacatgacgt cgaaggtgac gtcgacggac tggacgtcgt cgaaggcgtg gacggcctcg
     9121 cccacccggc gctggatctc gttcttcagg gggcagccgg ggacggtgag ggcgatctcg
     9181 acctggaccg actggccgtc gatccgcacg aagcgcacca tgccgaggtc cacgatgctg
     9241 cggtgcagct cggggtcctc caccggtcgg agcgcttcga tgatctgggc ttcggtgggc
     9301 acgtgggcac cttccaggtg gggctggcgt tcgtccgagg gatttcccct atcggcatag
     9361 gtagaatacc gatgtggatg cggactcacc caccccgctg agcgcggccg acttctcctc
     9421 ggcggtcacg gcgatcacgt cggccttcgg ggacccgacc cggcgcgaga tctacctgtg
     9481 gctccgcgac cagcccgagg gcgtcaccgc ctcggccgtg gccgagcgct tcgagctgca
     9541 ctccaacgtg gcccggcacc acctcgacaa gctggccggt ggcggctacg tcgaggtctc
     9601 cacggcccgg ctcgccggca gcggcgccgg ccgcccgtcg aagcggtaca aggcgatcgg
     9661 gaacgagaac ccgatcgagt tcgccgtccg ccacgacgac ctgctcgtca ccctgctcgg
     9721 caagtcgctg gcgctgctgc cgggcgatgt cgcggcccgc atggccgagg aggtcggagt
     9781 cgagtacggc cggtcgctcg ccgcgtcgat gggttcgggc gagcacgtgc agcgctcgtt
     9841 ccgctcggcg ctgcacgcgg tcgccgacgc cctgacctcc cacggcttcg ccgcccacgc
     9901 cgagaagcag ggcgacgagc tgcggatcgt ggcccagcac tgcccgttcg gcgatgccgc
     9961 catcgagcac ccggtgatct gcgccgtgga ccgcggcctg gtcaagggca tgctctcgtc
    10021 gctgtacggc gacacggcac cggcgacgga gaccacgcag gcgctcggct ccgagacctg
    10081 cgtcaccgtc gtcacgtccc gctgagccgg cctcctctcc ctgtgccgtc gcccgtgtcg
    10141 tcaccggacc tcggtcacta cctggaccac gcctccacgt cgccgctccg acccgaggcc
    10201 cgggcggcga tgatcgaggc gctgggcgcg ccgctggccg acccgtcccg catccacgaa
    10261 ccggggatga ccgcccgggt cgcgctcgag caggcgcggg aggtggtggc cgggttcctc
    10321 ggtgcccgcc ctcgcgaggt cgtgttcacg tccggcgcgt cggagtcgat cgctgcggcc
    10381 tgctggggtg cggccccccg aggccggacg caggtgctgt ccgccgtgga gcactcggct
    10441 gtgcgactcg cagccgagca gcacggcccc accgccgtcg tgggatgcga ccgcaccggc
    10501 cgggtcgacc cggacgagct gctggccgcc gtcgatgacg acactgccct cgtgcacctg
    10561 cagctcggca accacgaggt cggcaccacg cagccggtgg ctgaggtcgt ggcggcgtgc
    10621 cgcgagcgcg gggtcctggt ccacgtcgac gccgcccaag ccgccgggcg tgttccgttc
    10681 gagttcgggg cgctcggtgc ggatctggtc tcggtcagcg gacacaagct gggcggcccg
    10741 gccgggaccg gggcgctgtt ggtgcgccgg gggctgcgga tccgcccgct gatcgtgggc
    10801 ggcgagcagg agcgg
//
LOCUS       DATISU010000040         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_467543, whole genome shotgun sequence.
ACCESSION   DATISU010000040 DATISU010000000
VERSION     DATISU010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467543"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VLR27_01900"
     CDS             complement(<1..951)
                     /locus_tag="VLR27_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HSP02225.1"
                     /translation="MSASRTSDLWWKNGIIYCLDVETFLDTDGDGTGDLPGLTDRVDY
                     LAGMGVTCIWLMPFYPSPNRDDGYDITDYYAVDPSLGSLGDLVAFIRTAKDRGMRIIV
                     DLVVNHTSDQHPWFRSARRSKDSPHRDWYVWAEEEPKEPHAENVFPDAEDSIWTKDDK
                     TGEWYLHHFYSHQPDLNIGNPAVREEIAKIAGFWLQVGVDGFRVDAVPFLIETEATDA
                     GGELSLDPHELIRDLRAFMARRRGDSVLLGEVNLPPEGLVEFFGDDDELELLFDFPLM
                     QAMYLALARCDAGPIRGALEARPEIPEDAQWAIFVRNHDEL"
     gene            complement(948..1931)
                     /locus_tag="VLR27_01905"
     CDS             complement(948..1931)
                     /locus_tag="VLR27_01905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03885 family FMN-dependent LLM class
                     oxidoreductase"
                     /protein_id="HSP02226.1"
                     /translation="MTRVGFHASHEQLAPSALLQAAKRADQAGFDDVSCSDHIAPWGE
                     RQGHSGFAWSWLGAALEATSVRCGVVNAPVQRYHPAIIAHAIATLAEMYPGRFWTALG
                     SGEALNEHITGERWPDKPTRDRRLLECVDIIKALLAGEEVTHDGLVTVDRAQLWDLPA
                     EPPMLVGAAVSEGTARTVGGWADALITINQPHDVLRKVFDAFREGGGEGKPIFLQVHL
                     SYDPDEATAEAVAHEQWRTNVFDSNLAWNLEMPAQFDAAAVHVTPADVREKVLVSSDL
                     GQQVAWLQEYVELGADSLYLHHVGQTQDAFIDAFAEHVLPSLQTDDTRSDR"
     gene            complement(1973..>2217)
                     /locus_tag="VLR27_01910"
     CDS             complement(1973..>2217)
                     /locus_tag="VLR27_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HSP02227.1"
                     /translation="ASVMTHVEGLRDRHLLLVHGLIDENVHFRHTARLINALVRADID
                     HRLLLYPDERHVPRGEADRESMEHRIAEFFRTTLTP"
BASE COUNT          303 a          719 c          841 g          354 t
ORIGIN      
        1 gagctcgtcg tggttgcgca cgaagatggc ccactgggcg tcctcgggga tctccggtcg
       61 ggcctccagc gcaccgcgga tcgggccggc gtcgcagcgg gccagcgcca ggtacatggc
      121 ctgcatgagc gggaagtcga agagcagctc cagctcgtcg tcgtcgccga agaactcgac
      181 caggccctcc gggggcaggt tgacctcgcc gagcaggacc gagtcgcccc ggcgccgggc
      241 catgaacgct cgcaggtcgc ggatcagctc gtgggggtcg agcgacagct cgccgccggc
      301 gtccgtggcc tcggtctcga tgaggaacgg caccgcgtcg acccggaagc cgtcgacccc
      361 cacctggagc cagaagccgg cgatcttggc gatctcctcc cgcactgccg ggttgccgat
      421 gttgaggtcg ggctggtgcg agtagaagtg gtgcaggtac cactcgccgg tcttgtcgtc
      481 cttggtccag atgctgtcct cggcgtcggg gaagacgttc tcggcgtggg gctccttcgg
      541 ctcctcctcg gcccagacgt accagtcacg gtgggggctg tccttggagc gccgggcgct
      601 gcggaaccac gggtgctggt cggaggtgtg gttgacgacc aggtccacga tgatccgcat
      661 gccccggtcc ttggccgtgc ggatgaacgc caccaggtcg ccgagtgaac ccaggctggg
      721 gtcgaccgcg tagtagtcgg tgatgtcgta gccgtcgtcc cggttggggg aggggtagaa
      781 gggcatcagc cagatgcacg tgacccccat cccggccagg tagtcgaccc ggtcggtgag
      841 gccggggagg tcgccggtcc cgtcaccgtc ggtgtcgagg aaggtctcga cgtcgaggca
      901 gtagatgatc ccgttcttcc accacaggtc gctggtgcgt gaggcgctca tcggtcgctc
      961 cttgtgtcgt cggtctgcag cgagggcagg acgtgctccg cgaaggcgtc gatgaaggcg
     1021 tcctgggtct ggccgacgtg gtggaggtag agcgagtcgg cgccgagctc gacgtactcc
     1081 tgcagccagg cgacctgctg gccgaggtcg gacgacacga gcaccttctc ccgcacgtcg
     1141 gctggggtga cgtggacggc ggcggcgtcg aactgcgccg gcatctcgag gttccaggcc
     1201 aggttggagt cgaacacgtt ggtgcgccac tgctcgtggg ccacggcctc ggcggtcgct
     1261 tcgtcggggt cgtaggacag gtgcacctgc aggaagatcg gcttgccctc gccaccgccc
     1321 tcgcggaacg cgtcgaagac cttccggagc acgtcgtggg gctggttgat cgtgatgagg
     1381 gcgtcggccc agccgccgac cgtgcgggcg gtcccttcgc tgacggcggc gcccaccagc
     1441 atcgggggct cggcgggcag gtcccagagc tgcgcccggt cgacggtgac gaggccgtcg
     1501 tgggtgacct cctcgccggc cagcagcgcc ttgatgatgt cgacgcactc gagcaggcga
     1561 cggtcccgcg tcggcttgtc cggccaccgc tcaccggtga tgtgctcgtt cagcgcctcg
     1621 cccgacccga gggcggtcca gaaccggccc gggtacatct cggcgagggt ggcgatggcg
     1681 tgggcgatga tcgccgggtg gtagcgctgc accggggcgt tgaccacgcc gcagcgcacc
     1741 gaggtggcct cgagcgcggc gccgagccag gaccaggcga agcccgagtg gccctgccgc
     1801 tcgccccacg gcgcgatgtg gtccgagcag ctcacgtcgt cgaagccggc ctggtcggcc
     1861 cgtttcgcgg cctggaggag ggcgctgggg gcgagctgct cgtgggacgc gtggaagccg
     1921 actcgtgtca tggccggtgt ccgtacccac ggccccgccg cggcacccac ggtcaggggg
     1981 tcagggtggt gcggaagaac tcggcgatgc ggtgctccat cgactcccga tcggcctcgc
     2041 cgcggggcac gtggcgctcg tcggggtaca gcaggagccg gtggtcgatg tcggcccgca
     2101 ccagcgcgtt gatcagccgg gcggtgtggc ggaagtgcac gttctcgtcg atcagcccgt
     2161 gcacgaggag caggtgccgg tcgcgcaggc cctcgacgtg ggtcatcacc gaggcgt
//
LOCUS       DATISU010000041         2586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_700659, whole genome shotgun sequence.
ACCESSION   DATISU010000041 DATISU010000000
VERSION     DATISU010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700659"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..570
                     /locus_tag="VLR27_01915"
     CDS             142..570
                     /locus_tag="VLR27_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSP02228.1"
                     /translation="MPTVLIATDNDAVADEVSAAIEDAETTIVRLRAGVEVPPAVEAH
                     QPDLVVLDLQIGNMGGMAVATHLRAEIAMERLDDVAILMLLDRTADVFLAKRSSADGW
                     LVKPLDSFRLRRAAKALLAGGDFHEATGIESVPPVEPASA"
     gene            620..1294
                     /locus_tag="VLR27_01920"
     CDS             620..1294
                     /locus_tag="VLR27_01920"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HSP02229.1"
                     /translation="MELRQRLAEHLPMFALEVRTPRLVLRYPDDQDALALAELGARGV
                     HDPDAHPFRIPWTSVPPPHQQRNTLSFLWDQRATAQRDDWHLPLVTIVAGEVVGTQDV
                     LASSWSVLRQVQTGSWLGQAHQGKGIGSEMRAAVLHLAFAGFGAVRASTGAWEDNPSS
                     LAVTRRLGYRPNGDVWDVRDGEAVRMHRFVLDRDDWEPTRRGDIELVGAEAVAELFGT
                     ERPPGS"
     gene            1371..1444
                     /locus_tag="VLR27_01925"
     tRNA            1371..1444
                     /locus_tag="VLR27_01925"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1405..1407,aa:Pro,seq:cgg)
     gene            1597..2496
                     /locus_tag="VLR27_01930"
     CDS             1597..2496
                     /locus_tag="VLR27_01930"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSP02230.1"
                     /translation="MHEAQLLAVLDGLPIAVMLRAADGSLLHANPATERFLARLGLDV
                     SHVAPSPSAMMDHVAVVDERGRRQDRDHLPVVSAIRDGSEREATLGYALPDGGWAWYA
                     TKAGPLTLTDGTIGTVVTCDDVTDRRLLEEELRTAALVDPLTGLHNRRALSAALDDAQ
                     EFRARAGGDVGLLFVDLDGFKTVNDTFGHDAGDRVLVETGTRLVAATRSVDRVCRVGG
                     DEFAILCAPIDGAEGLQELVTRIRSLPPRPVLVQGEALAVLGSIGAVLVEPGDRLDQA
                     LLRADIAMYDHKRSNDASLLVIG"
BASE COUNT          349 a          919 c          925 g          393 t
ORIGIN      
        1 tcggcgtcgg ccacgccccc gagggcgttg cggaactcgt cgaccgtgta ggcgtcgagc
       61 tcgccgctcg ggcggcacag cgtgtagtcc ggggtctgct cgatctcgat gtccatcgcg
      121 gggcacgcta ccgttcctgc cgtgcccacg gtcctcatcg ccaccgacaa cgacgccgtc
      181 gccgacgagg tctcggcggc catcgaggac gccgagacca ccatcgtgcg cctgcgcgcc
      241 ggcgtcgagg tgcccccggc cgtggaggcc caccagcccg acctggtggt gctcgatctg
      301 cagatcggca acatgggcgg catggccgtg gccacgcacc tgcgggccga gatcgccatg
      361 gagcgcctcg acgacgtggc catcctgatg ctgctcgatc gcaccgccga cgtgttcctg
      421 gccaagcggt ccagtgccga cggctggctg gtgaagccgc tcgactcgtt ccgcctgcgc
      481 cgggcggcca aggcgctgct cgccggcggc gacttccacg aggccaccgg catcgagtcg
      541 gtgccgccgg tggagcctgc ctcggcctga tcggtcgaat ccgctggcgt ccggtgggcg
      601 tgggcggcac gctcgggtga tggagctgcg ccagcgactc gcggagcacc tgccgatgtt
      661 cgccctcgag gttcggacgc cgcgcctggt gctgcggtac cccgacgacc aggacgcgct
      721 ggcgctggcc gagctcggcg cacgaggcgt gcacgatccg gacgcccacc cgttccgcat
      781 cccgtggacg tcggtcccac cgccgcacca gcagcgcaac accctgtcgt tcctctggga
      841 ccagcgggcc accgcccagc gcgacgactg gcacctcccg ctcgtcacga tcgtggccgg
      901 ggaggtggtc ggcacccagg acgtgctggc gtcgtcgtgg tcggtgctgc gccaggtgca
      961 gaccgggtcc tggctcgggc aggcgcacca gggcaagggc atcggttccg agatgcgcgc
     1021 tgcggtgctg cacctggcgt tcgccggctt cggggcggtg cgggcgtcca ccggagcgtg
     1081 ggaggacaac ccgtcgtcgc tcgcggtgac ccgtcgtctg gggtaccggc ccaacgggga
     1141 cgtctgggat gtccgcgacg gcgaggccgt gcggatgcac cgcttcgtcc tggaccgtga
     1201 cgactgggag cccactcggc gcggtgacat cgagctggtg ggagccgaag ccgtcgccga
     1261 gctgttcggc accgaacggc cgccggggtc ctgacccgga tccggcttcg atcggcgccg
     1321 gacccctccg gtatggtggt caggccctgg ctccaccggc caggacgatc cgggtggtgg
     1381 ctcagcttgg cagagcgctg cgttcgggac gcagaggtcg ggagttcaaa tctcccccac
     1441 ccgactcgac gagacgtcag gccagcgagg gcttcgggcc ctcgctgcgc ctcgtccagc
     1501 gacgagcgcc gcctgtcggt cgctcaagat cggagccggt gacgtcgatg aacctccgtg
     1561 gagggctttc aacgggaacg acccggcttg ggtgaggtgc acgaggccca gctgctggcg
     1621 gtgctcgacg ggctccccat cgccgtgatg ctgcgggcag ccgacggctc cctgctccac
     1681 gccaacccgg ccacggagcg gttcctcgcc cggctggggc tcgacgtctc gcacgtggcg
     1741 cccagcccca gcgccatgat ggaccacgtc gccgtggtcg acgagcgcgg tcgccggcaa
     1801 gaccgcgacc acctcccggt cgtctcggcg atccgtgacg gctctgagcg cgaagcgacg
     1861 ttgggctacg cactcccgga tgggggctgg gcttggtacg ccacaaaggc gggaccgctg
     1921 acgctcaccg atggcacgat cggcacggtc gtcacctgcg atgacgtcac cgaccgtcgg
     1981 ctgctcgaag aggagctgcg caccgcggcg ctcgtcgacc cgctgacggg gctgcacaac
     2041 cgacgggccc tgagtgctgc cctcgacgac gcccaggagt tccgggctcg tgccggcggc
     2101 gacgtcggcc tcctgttcgt cgacctggac gggttcaaga cggtcaacga cacgttcggc
     2161 cacgacgccg gcgaccgggt gctcgtggag acaggcaccc gcctggtcgc ggccacccgg
     2221 tccgtcgacc gggtctgtcg ggtcggcggt gacgagttcg cgatcttgtg cgcaccgatc
     2281 gacggtgccg aagggctgca ggagctcgtg acgcgcatcc ggtcgctgcc gccgcggccc
     2341 gtcctcgtcc agggtgaagc ccttgcggtg ctcgggagca tcggcgccgt cctcgtcgaa
     2401 ccaggggacc gcctcgacca ggcacttctc cgtgctgaca tcgccatgta cgaccacaag
     2461 cgaagcaacg acgcctccct gctcgtcatc ggttgactcc ctcgagccaa caggtcgtgc
     2521 gccgaggcga gctgcggcga gccgttcgct ggttcgagtc atgacccgat ctaggttgcg
     2581 gaggtg
//
LOCUS       DATISU010000042         9154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_374612, whole genome shotgun sequence.
ACCESSION   DATISU010000042 DATISU010000000
VERSION     DATISU010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374612"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VLR27_01935"
     CDS             <1..634
                     /locus_tag="VLR27_01935"
                     /inference="COORDINATES: protein motif:HMM:NF027650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02231.1"
                     /translation="MAEPAGLLILDEPTNHLDLASCDMLEDALKVYPGTVLLVTHDRY
                     LIRSVADDLIEVRNGTATFHHGVDEAVLTPTTATTRRDTSGTPVKSAKKSGGRSGKAA
                     RAKGPTQPAKAPGQSSPNAPKQKPKAKQGGGGSQQDRDLRKQVNRLEKQWETAEAKVA
                     ELQVALADPDIYADADKLTELTTALDAAKDEAVRLMTDWEQAASRLERSS"
     gene            757..1659
                     /locus_tag="VLR27_01940"
     CDS             757..1659
                     /locus_tag="VLR27_01940"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HSP02232.1"
                     /translation="MGTTTRIDRLNQASARRVIEPDHDLPGSLGPGRVLPDELLSIHG
                     LDLDLTEDQRIRLGREELAASLRSGILFEAILEAGFAMQIAKSDDVTDPRITFLLHEI
                     GEETRHQRMFQRLVQQLQPTAVRREIGWVDRVLEAVGPHLIIALPALFHTLVLAGEEI
                     PDLLQKRSVEHPDTDPFLAEVNRYHRSEEARHLSYAKAVLPEVWEGANPIDRVAVRRV
                     APWVVNDMFRFLIGPGVYETVGLPGRETWEAVQQLPGRRALLHEAMRNVLATLIDAGA
                     LRPGHLPAGWQEVCGVDAGGRPRA"
     gene            1697..2287
                     /locus_tag="VLR27_01945"
     CDS             1697..2287
                     /locus_tag="VLR27_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02233.1"
                     /translation="MLDHVFTDAIGALRDALEAAMLERMTAEERFQVDVLLGDTTWET
                     SYGLPGEGNPPRVRADITLDWPTWSQSAFRSWLRNEELDDDPRIEISIVLRLQRLVEL
                     PRPDQVTAVLPVDSPSVGPDQLHRTAPTVETAYTAELEPVSHALELAYEGTYDLDDKA
                     LGDGSILDDHFSALGGWIASMLVRLGDLPLAYHDPY"
     gene            2308..3096
                     /locus_tag="VLR27_01950"
     CDS             2308..3096
                     /locus_tag="VLR27_01950"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HSP02234.1"
                     /translation="MSDPVVLVERTDAVATVTLNRPGARNALSQELRKSLWQAMSELE
                     ADVNVAAIVLTGADPAFCAGLDLKELASTAGAALGETGAAGDTASGRGPIPPMETPVI
                     GAINGVAITGGFEVALACDFLIASERAAFADTHARMGIMPGWGLTVLLPEAVGFRRAK
                     ELSTTGNFLDAQTALAWGLVNHVVPHDELVPFAQKLAADIATNDQAGVRQMLQTYAQG
                     ALITRGEAWALEGRVAGEWQAGGRDGADLEARRKAVTERGRSQV"
     gene            3098..3514
                     /locus_tag="VLR27_01955"
     CDS             3098..3514
                     /locus_tag="VLR27_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP02235.1"
                     /translation="MEPRISAITLGVQDVDRARRFYEALGWSGESPDGEVVFFQAGGL
                     VLALWSREELAHDSEVTDPGGWGGVTLAHNVGSPAEVDAVLTQAEAAGGTIARPGGKA
                     FWGGYTGIFIDPDGHPWEVAHNPIWRLDDDGTVHLH"
     gene            complement(3522..4511)
                     /locus_tag="VLR27_01960"
     CDS             complement(3522..4511)
                     /locus_tag="VLR27_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP02236.1"
                     /translation="MTKAFGDLAVLRDVDLAVDDGSTLSLLGPSGSGKSTLLRIVAGL
                     TPPDAGSVHLGADDVTDRPAHRRGVGVVFQDQALFPHLDVGGNVGFGLRMAGVDRAER
                     AERVRDALGLVGLSGYERRDPATLSGGEAQRVALARALAPRPAVILLDEPLAALDQLL
                     RERLLVELRALFRELDVTVVAVTHDPTEAAALGDRVAVLLDGAIAQVGTPGEVIAAPV
                     SAAVAGLVGQRNVLAGRVVEGGVETALGVLPVDAGRARGEVDVLVPPSALVPAPEGGV
                     RSVVCGLLYRSGTPVAVVDAGGVMLDVLAEPRWAVGDAVALDVDPAGVHVMGR"
     gene            complement(4537..6210)
                     /locus_tag="VLR27_01965"
     CDS             complement(4537..6210)
                     /locus_tag="VLR27_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HSP02237.1"
                     /translation="MNPTARPARASRTVTAAAFAVPVVFLGVFFAVPVATLVSTGLRE
                     GGRWEVGAVGEVLTSSSTRSVAWFTLWQAVLSTVATVVAALPAAWLIGRIRFPGRSLF
                     VAALIVPFVLPTVVVGTAFLAVLGPDGPVAALGGVAGFDVDLRRTATAVILAHVFFNY
                     AVVARTVGAAWMAVDPAAEDAARTLGADRWTTFRTVTLPQLRPALAGAASIVFLFTFT
                     SFGVVKILGGPRIATIEVEIHRLTTQLLDLRTASVLSLLQLAAVIAALALYRRAGARR
                     GSARTRAGRSVLHRPAGAERWAMAATVTSAALLVGVPIATLVWRSVDTAGGPGLGYYR
                     ALGSTGRGSALFVPPAEAVRNSLVIGAAAATTALVVGGLAAVGLHRLGGRARGIADSS
                     LMLPLGVSAVTVGFGFLIALDEPPLDLRTSPVLIPAAQAVIAIPFVVRTLLPALDAVP
                     ARLREVAAALGAPPHRVWWEVDARLLRRPALGALGFAYAISLGEFGATVFIARADAPT
                     VPVAIFRALSQPGALNVGQAMALSTILLTLTAAVMLLVDRVGDARVGGF"
     gene            complement(6218..7288)
                     /locus_tag="VLR27_01970"
     CDS             complement(6218..7288)
                     /locus_tag="VLR27_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HSP02238.1"
                     /translation="MTPTRPLLALLAAALVVAGCGDDDAETTPEPEGGDATSITMLTH
                     DSFDVSDEVLAAFTEETGITVELLPSGDAGSMLNQAILTKGSPQGDVLFGVDSTFLSR
                     ALEEDLFVAYESPALDGVDESLVLDPEHRVTPIDTGDVCLNYDKAGLADAGLEPPEDL
                     ADLADPAYAGTLVVENPATSSPGLAYLLATVAEFGEDGWQEHWQALRANDVEVAAGWE
                     EAYYGSFSGAAGSPGDRPIVVSYASSPPAEVIFAEEPLDDAPTGVVEGSCYRQIEFAG
                     ILAGTEHEEAAQQLIDFLLSPTFQEDVPLNMFVYPVIDVELPAEFVEHGAEPEDPYLL
                     DADVVAENRDAWIQEWTDIVLR"
     gene            complement(7285..7962)
                     /locus_tag="VLR27_01975"
     CDS             complement(7285..7962)
                     /locus_tag="VLR27_01975"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HSP02239.1"
                     /translation="MDLDRPPDIVVVAGGEPVAPALVAVVPASARVVAADAGVDLARS
                     LGWEVHVAVGDFDSVSDDGRSALTSEVADVRVHPMAKDATDLELALEVAAELAPAPPA
                     AGRRVLVIGLEGGRPDHALANLLVAAAEPFAELELELVLEHGRAWVVRDRLTGRLPAG
                     GLVSLVPVHGPATVSVRGVRWPLDRAPLPAGTTRGVSNEALGGPFDLQVHDGAVLCIT
                     PRPEETP"
     regulatory      complement(7951..8064)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8234..>9154
                     /locus_tag="VLR27_01980"
     CDS             8234..>9154
                     /locus_tag="VLR27_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HSP02240.1"
                     /translation="MLNRRWMRLLALLLGLSLIAAACGDDDDTDTSTEDTEDTTEGAA
                     GDGGEGAESDFLACEVTDTGGVDDRSFNENAYNGLVEAADEIGFEPVVLESQSDADFD
                     PNIQALIDQSCDLIVTVGFLLGDATEAAAQENPDTNFAIVDYAYETEYENVLGLTYAT
                     DQAAFMAGYVAAAQTQTGTVGTYGGLNIPTVNIFMKGFEAGIEYHNEQKGTDVQLEGW
                     STADDDGLFTGDFEDLSKGRQTTESLLDQGADIILPVAGPVGQGSVEAIRAGGDDAKL
                     IWVDTDGCESVADACDLFLTSVMKNFDVSVY"
BASE COUNT         1393 a         3354 c         3314 g         1093 t
ORIGIN      
        1 gatggccgag ccggccggcc tgctgatcct cgacgagccc accaaccacc tcgacctggc
       61 cagctgcgac atgctcgagg acgccctcaa ggtgtacccg ggcacggtcc tgctcgtcac
      121 ccacgaccgc tacctgatcc gctcggtggc cgacgacctc atcgaggtcc gcaacggcac
      181 ggcgacgttc caccacggcg tggacgaggc cgtgctcacg cccacgacgg cgaccacccg
      241 gcgggacacg tcggggacgc cggtgaagag cgcgaagaag agcggtggcc gctcgggcaa
      301 ggccgccagg gccaagggtc cgacccagcc ggcgaaggcc cccgggcaga gcagcccgaa
      361 cgcacccaag cagaagccca aggcgaagca gggtggtggc ggcagccagc aggaccgcga
      421 cctgcgcaag caggtgaacc ggctcgagaa gcagtgggag acggctgagg ccaaggtcgc
      481 cgagctccag gtggcgctgg ccgacccgga catctacgcc gatgccgaca agctcaccga
      541 gctcacgacc gccctcgacg cggccaagga cgaggccgtg cgcctgatga ccgactggga
      601 gcaggccgcc tcccggctcg agcgttcgag ctgaggctgt gaaccgggcc gcggtgcacg
      661 catcggatgc gtgtggggcg gcccggaacg gggcgtgaat ctggtcaccc ggggctgaca
      721 ccgaaccggc accaatggtt cacttcggac atggggatgg ggacgaccac gcgcatcgac
      781 cggctcaacc aggcatcggc tcgtcgggtg atcgaacccg accacgacct gcccggctcg
      841 ctcggaccgg gacgggtgct gcccgacgag ctgctcagca tccacggcct cgacctggac
      901 ctcaccgagg accagcgcat ccggttgggg cgtgaggagc tggcggcgtc gttgcgctcg
      961 gggatcctgt tcgaggccat cctcgaggcc ggcttcgcca tgcagatcgc caagagcgac
     1021 gacgtcaccg acccccggat cacgttcctg ctgcacgaga tcggcgagga gacccgccac
     1081 cagcgcatgt tccagcgact ggtccagcag ctgcagccca ccgccgtccg tcgcgagatc
     1141 ggctgggtcg accgggtgct ggaggccgtc ggcccgcacc tgatcatcgc cctgcccgcc
     1201 ctcttccaca cgctcgtgct ggccggcgag gagatccccg acctgctcca gaagcgctcg
     1261 gtcgagcacc ccgacaccga tccgttcctg gccgaggtca accgctacca ccgcagcgag
     1321 gaggcccggc acctgtccta cgccaaggcg gtgctgcccg aggtctggga gggggcgaac
     1381 ccgatcgatc gcgtcgcggt ccggcgcgtc gccccctggg tcgtgaacga catgttccgc
     1441 ttcctcatcg gccccggcgt gtacgagacc gtcggtctcc ccggccggga gacctgggag
     1501 gccgtgcagc agctccccgg tcggcgggcc ctgctccacg aggcgatgcg caacgtgctc
     1561 gcgaccctga tcgacgccgg ggcgctgcgg ccagggcacc tgccggcggg ctggcaggag
     1621 gtctgcggcg tcgatgcggg cggtcggccc cgggcctgac ggtcggccgc gggcgacccc
     1681 ctagagtcgc cgacccatgt tggaccacgt cttcaccgac gcgatcggag ccctgcgcga
     1741 cgccctcgag gcggcgatgc tcgagcggat gacggcggag gagcggttcc aggtcgacgt
     1801 gctgctcggc gacaccacgt gggagacctc gtacggcctc ccgggcgagg gcaacccccc
     1861 gcgggtgcga gccgacatca cgctcgactg gccgacgtgg tcgcagtcgg cgttccggtc
     1921 gtggctgcgc aacgaggagc tcgacgacga cccccgcatc gagatcagca tcgtgctccg
     1981 gctccagcgc ctggtcgagc tcccccggcc cgaccaggtg accgccgtgc tccccgtcga
     2041 cagcccgtcg gtcggcccgg accagctgca ccgcaccgcg ccgacggtgg agacggcgta
     2101 cacggccgag ctggagccgg tcagccacgc cctcgagctg gcctacgagg ggacctacga
     2161 cctggacgac aaggcgctcg gcgacggatc catcctcgac gaccacttct ccgccctcgg
     2221 cggctggatc gcctcgatgc tcgtccgcct cggcgacctc ccgctcgcct accacgaccc
     2281 ctactgacct gacccacgga gcccgccatg tccgatcccg tcgtcctcgt cgagcgcacc
     2341 gacgccgtcg ccaccgtcac cctcaaccgg cccggcgccc gcaacgccct gagccaggag
     2401 ctgcgcaagt ccctgtggca ggcgatgtcc gagctcgagg ccgacgtgaa cgtggcggcc
     2461 atcgtcctca ccggcgccga ccccgccttc tgcgccggcc tggacctgaa ggagctggcg
     2521 tcgaccgccg gcgccgcgct gggcgagacg ggtgccgccg gtgacaccgc cagcggccgg
     2581 ggcccgatcc cgccgatgga gacccccgtc atcggcgcga tcaacggcgt ggccatcacc
     2641 ggtggcttcg aggtcgccct ggcctgcgac ttcctgatcg cgtccgagcg ggctgcgttc
     2701 gccgacaccc acgcccgcat ggggatcatg cccggctggg gcctgaccgt cctgctgccc
     2761 gaggccgtgg gcttccggcg agccaaggag ctgtcgacca ccggcaactt cctcgatgcg
     2821 cagaccgcgc tggcgtgggg cctggtcaac cacgtggtgc cccatgacga gctcgtgccc
     2881 ttcgcccaga agctcgccgc cgacatcgcc accaacgacc aggccggcgt ccgccagatg
     2941 ctgcagacct acgcgcaggg tgcgctgatc acccgcggcg aggcgtgggc gctcgagggt
     3001 cgggtcgccg gcgagtggca ggccggcgga cgcgatggcg ccgacctcga ggcccggcgc
     3061 aaggcggtca ccgagcgggg ccgctcccag gtctgacgtg gaaccgcgca tctcggccat
     3121 caccctcggc gtgcaggacg tcgatcgggc ccggcgcttc tacgaggcgc tcggctggtc
     3181 tggcgagtcc cccgacggcg aggtcgtgtt cttccaggcc ggtgggctgg tcctcgccct
     3241 gtggagccgg gaggagctgg cccacgacag cgaggtcacc gatcccggcg gctggggcgg
     3301 cgtgaccctg gcgcacaacg tcggctcccc ggcggaggtg gatgcggtgc tcacccaggc
     3361 cgaggcggcg ggtgggacca tcgcccggcc cggcgggaag gccttctggg gcggctacac
     3421 cgggatcttc atcgatcccg acggccaccc gtgggaggtc gcccacaacc cgatctggcg
     3481 cctcgacgac gacgggaccg tccacctcca ctgaccaggc gtcaccggcc catcacgtgg
     3541 acgccggcgg ggtcgacgtc gagcgcgacc gcatcgccga cggcccaccg gggctcggcc
     3601 agcacgtcga gcatcacccc gccggcgtcg accaccgcga ccggcgtgcc gctgcggtag
     3661 agcaacccgc agacgacgct ccgcaccccg ccttccggag ccgggaccag cgccgacggc
     3721 ggcaccagga cgtcgacctc accgcgggcg cgacccgcat cgaccgggag gaccccgagg
     3781 gcggtctcca caccgccctc cacgacgcgc cccgccagca cgttccgctg gccgacgagg
     3841 ccggcgacgg cggcggagac cggtgcggcg atgacctcgc ccggcgtccc cacctgggcg
     3901 atggcgccgt cgagcagcac cgcgacgcgg tcgccgagcg cggcggcctc ggtggggtcg
     3961 tgggtcacgg cgacgacggt gacgtcgagc tcacggaaca gcgcccgcag ctccaccagc
     4021 aggcgctccc gcagcagctg gtccagcgcg gcgaggggtt cgtcgaggag gatcaccgcc
     4081 ggccggggtg ccagcgcccg ggccagcgcg acccgctggg cctccccgcc ggagagggtc
     4141 gccgggtcgc ggcgctcata gccggacagg ccgacgagcc cgagggcgtc ccggacccgc
     4201 tcggcccgct ccgcccggtc gacgcccgcc atccgaaggc cgaagccgac gttgccgccg
     4261 acgtcgaggt gcgggaacag cgcctggtcc tggaagacga cgccgacgcc ccggcggtgg
     4321 gcgggccggt cggtgacgtc gtcggcaccc aggtgcacgg agccggcgtc gggcggggtg
     4381 aggccggcga cgatgcgcag cagcgtggac ttgccgctgc cggacggacc gagcagcgac
     4441 agcgtgctgc cgtcgtcgac ggcgaggtcc acgtcgcgca ggaccgccag gtcgccgaag
     4501 gccttcgtca cgttccgcac ggtcagggac gacacgtcag aagccgccga cccgcgcgtc
     4561 gccgacccgg tccacgagca gcatcaccgc ggcggtcagg gtcagcagga tggtggacag
     4621 cgccatggcc tggccgacgt tcagcgcccc gggctgggag agcgcccgga agatggccac
     4681 gggcacggtc ggtgcgtcgg cccgggcgat gaacaccgtc gcgccgaact ccccgagcga
     4741 gatggcgtag gcgaagccga gcgccccgag cgccggccgg cgcagcaggc gggcgtccac
     4801 ctcccaccac acccggtgcg gtggggcgcc gagcgcagcc gccacctccc gcagccgggc
     4861 cgggaccgcg tcgagcgcgg gcaggagcgt gcggacgacg aacgggatgg cgatcacggc
     4921 ctgggccgcg gggatcagca ccggcgaggt ccgcaggtcg agcggcggct cgtcgagcgc
     4981 gatgaggaac ccgaagccga cggtgacggc gctgacgccg aggggcagca tgagcgacga
     5041 gtctgcgatc ccgcgggccc gcccgccgag ccggtgcagg ccgacggccg ccagcccgcc
     5101 gaccacgagc gcggtcgtgg ctgccgccgc gccgatgacc agcgagttcc ggaccgcctc
     5161 ggccggtggc acgaagagcg cgctcccccg gccggtggac cccaacgccc ggtagtagcc
     5221 cagcccgggg ccgccggcgg tgtcgacgga gcgccagacg agggtggcga tggggacgcc
     5281 cacgagcagg gccgccgagg tgacggtggc ggccatcgcc cagcgctcgg caccggccgg
     5341 gcggtggagg accgagcggc cggcacgggt gcgggcactt ccccggcggg cgccggcccg
     5401 tcggtacagg gccagggcgg cgatgacggc ggcgagctgg agcagggaca gcaccgacgc
     5461 ggtgcgcagg tcgagcagct gggtggtgag gcggtggatc tcgacctcga tcgtcgcgat
     5521 gcgtggcccg ccgaggatct tgacgacccc gaaggaggtg aaggtgaaca ggaagacgat
     5581 ggacgccgcc ccggccaggg ccgggcgcag ctggggcagc gtgacggtgc ggaacgtcgt
     5641 ccaccggtcg gcgccgaggg tgcgggcggc gtcctcggcg gcgggatcga cggccatcca
     5701 cgccgcgccc accgtgcggg cgaccacggc gtagttgaag aacacgtggg cgaggatcac
     5761 cgcggtggcc gtgcggcgca ggtcgacgtc gaacccggcc acgccgccga gcgcggccac
     5821 cggaccgtcg gggccgagca cggccaggaa ggccgtgccg accacgacgg tgggcaggac
     5881 gaacgggacg atgagggcgg ccacgaacag cgagcggccc gggaaccgga tgcgcccgat
     5941 cagccacgcc gccggtaggg cggcgacgac cgtggccacg gtggagagca ccgcctgcca
     6001 gagcgtgaac caggccaccg accgggtgct cgacgacgtc agcacctcgc cgacggcgcc
     6061 gacctcccac cggcccccct cgcggagccc ggtggagacc agcgtcgcca ccggcacggc
     6121 gaagaagaca ccgaggaaca ccaccggcac ggcgaacgcc gcggcggtga cggtccgcga
     6181 cgctcgtgct ggtcgcgcgg tggggttcac ggtccgatca tcgcagcacg atgtcggtcc
     6241 actcctggat ccaggcgtct cggttctccg ccaccacgtc ggcgtcgagc aggtacgggt
     6301 cctcgggctc ggccccgtgc tcgacgaact cggcgggcag ctccacgtcg atcaccgggt
     6361 acacgaacat gttcagcggc acgtcctcct ggaaggtggg cgagagcagg aagtcgatca
     6421 gctgctgggc ggcctcctcg tgctccgtgc cggccaggat cccggcgaac tcgatctggc
     6481 ggtagcacga gccctccacc acgccggtgg gggcgtcgtc cagcggctcc tcggcgaaga
     6541 tgacctcggc cggcgggctg gacgcgtagc tcaccacgat gggccggtcg ccggggctgc
     6601 cggcggcgcc ggagaagctg ccgtagtacg cctcctccca gccggcggcc acctcgacgt
     6661 cgttggcgcg cagggcctgc cagtgttcct gccacccgtc ctcgccgaac tcggcgaccg
     6721 tggcgagcag gtaggccaag cccggtgacg acgtcgccgg gttctcgacc acgagcgtgc
     6781 cggcgtaggc gggatcggcc agatcggcca gatcctcggg cggctccagg ccggcgtcgg
     6841 cgagacccgc cttgtcgtag ttgaggcaca cgtcgccggt gtcgatcggc gtcacccggt
     6901 gctcggggtc gagcacgagc gactcgtcga ccccgtcgag cgccggcgac tcgtaggcga
     6961 cgaacaggtc ctcctcgagg gcccgggaca ggaacgtgct gtcgacgccg aacaggacgt
     7021 cgccctgcgg tgagcccttg gtgaggatcg cctggttcag catggagccg gcatcgccgg
     7081 acggcagcag ctcgaccgtg atgccggtct cctcggtgaa ggcggccagc acctcatcgg
     7141 acacgtcgaa gctgtcgtgg gtgagcatcg tgatcgaggt ggcgtcgccg ccctcgggct
     7201 ccggcgtggt ctcggcgtcg tcgtcaccgc agccggcgac gaccagcgca gcagcgagca
     7261 gggcgagcag gggtcgggtc ggggtcacgg ggtctcctcg ggtcgggggg tgatgcagag
     7321 gacggcgccg tcgtggacct gcaggtcgaa cgggccgccg agggcttcgt tgctgacgcc
     7381 tcgggtggtg ccggcgggga gcggggcgcg gtcgagcggc cagcgcaccc cgcggaccga
     7441 cacggtcgcc gggccgtgga cgggcaccag cgacaccagg ccgcctgccg gcagccggcc
     7501 ggtgaggcgg tcccggacca cccacgcccg gccgtgctcc aggacgagct cgagctcgag
     7561 ctcggcgaag ggctcggccg cggcgacgag caggttggcg agggcgtggt cgggtcgacc
     7621 cccctcgagg ccgatcacca gcacccggcg gccggccgca ggaggtgcgg gcgcgagctc
     7681 ggcggcgacc tccagcgcga gctcgaggtc ggtggcgtcc ttggccatcg ggtgcacgcg
     7741 cacgtcggcc acctcggagg tgagcgcgga ccgcccgtcg tcggacaccg aatcgaagtc
     7801 cccgaccgcc acgtggacct cccagcccaa cgagcgggcc agatccacgc cggcgtcggc
     7861 ggcgacgacc cgagccgagg cggggacgac ggccacgagg gcgggcgcga ccggctcgcc
     7921 tccggcgacg acgacgatgt caggtggacg atcgaggtcc acgacgttcc ctccgctggc
     7981 attacccaga tcaggttcca gggtcggtga cgcctcgcag ccaccctctc agcccggttg
     8041 ccccgagctc cccggcgccg gttcttctgc ggcgcctccc cgaccatacc cgacccatct
     8101 gtcgggacct tggtcacctg atgcgcctgg atcattgtcc cggaccccgc cgagacctac
     8161 cgttgcgccg tcccctgaca tttcacgggt atgcgcacat ccgttgcgtg accccacccg
     8221 gaggtagaac cctgtgctga accgacgctg gatgcggctg ctcgcgctgc tgctcggcct
     8281 gtccctcatc gctgccgcgt gcggcgatga cgacgacacc gacacgtcca ccgaagacac
     8341 cgaagacacg accgagggcg cagccggcga cggcggcgag ggcgcggaga gcgacttcct
     8401 ggcctgcgag gtcaccgaca ccggcggcgt cgacgaccgc tccttcaacg agaacgccta
     8461 caacggcctg gtcgaggcgg ccgacgagat cggcttcgag ccggtcgtgc tcgagtccca
     8521 gagcgacgcc gacttcgatc ccaacatcca ggcgctcatc gaccagagct gcgacctgat
     8581 cgtcacggtc ggcttcctgc tgggcgacgc caccgaggcg gccgcgcagg agaaccccga
     8641 cacgaacttc gcgatcgtcg actacgcgta cgagaccgag tacgagaacg tcctcggcct
     8701 gacctacgcc accgaccagg ccgccttcat ggccggctac gtcgccgcgg cgcagaccca
     8761 gaccggcacg gtcggcacgt acggcggcct caacatcccc accgtgaaca tcttcatgaa
     8821 gggcttcgag gccggcatcg agtaccacaa cgagcagaag ggcaccgacg tccagctcga
     8881 gggttggagc accgccgacg acgacggtct tttcaccggt gacttcgagg acctctcgaa
     8941 gggccgccag accaccgagt cgctgctcga ccagggtgcc gacatcatcc tcccggtggc
     9001 cggccccgtg ggtcagggct ccgtcgaggc gatccgcgcc ggcggcgacg acgccaagct
     9061 gatctgggtc gacaccgacg gctgcgagtc cgtcgccgac gcctgcgacc tgttcctgac
     9121 cagcgtcatg aagaacttcg acgtgtcggt ctac
//
LOCUS       DATISU010000043         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_654053, whole genome shotgun sequence.
ACCESSION   DATISU010000043 DATISU010000000
VERSION     DATISU010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654053"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..291)
                     /gene="yidD"
                     /locus_tag="VLR27_01985"
     CDS             complement(49..291)
                     /gene="yidD"
                     /locus_tag="VLR27_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HSP02241.1"
                     /translation="MIARTLDRLVWLYQKATEGRMSPCRYVPSCSTYAREALAHHGAG
                     RGTWLTVRRIVRCAPWGGHGYDPVPGVPEPASHHPR"
     gene            complement(288..632)
                     /locus_tag="VLR27_01990"
     CDS             complement(288..632)
                     /locus_tag="VLR27_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HSP02242.1"
                     /translation="MVAPVRSREDFAALSRSRARGSSGPIWIVHAPPTDAEHPVAQVA
                     YAVSKKVGSAVVRNRVRRRLRPLMDDLDAEGVLPAGGYLVGVRPDVVGLPTVELRRHL
                     ARAVASSTGVRS"
     gene            complement(638..772)
                     /gene="rpmH"
                     /locus_tag="VLR27_01995"
     CDS             complement(638..772)
                     /gene="rpmH"
                     /locus_tag="VLR27_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HSP02243.1"
                     /translation="MKRTYQPKKQRRARRHGFRHRMATRAGRAVVRNRRQKGRAKLSA
                     "
     gene            1188..>2197
                     /gene="dnaA"
                     /locus_tag="VLR27_02000"
     CDS             1188..>2197
                     /gene="dnaA"
                     /locus_tag="VLR27_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HSP02244.1"
                     /translation="MSDAETVWKGCAALLREQVSGVVWSSSFQNVEPLSYDGTTLVLG
                     FPSTVVRDRVQERYLSLLRANLSEMGLDVAVTLEARAATPQEVPLFDELADIRAATAE
                     APVADNGQRAPSGIAPYSPDSSEGRSTLNDKYTFDAFVTGNSNRFAHAASLRVAETPA
                     RSYNPLFIYGDAGLGKTHLLQAIGNYVLENYPTYKVRYVTSEQFLSEFVDSIRTGTNE
                     AFKRRFRDIDVLMVDDIQFFEGKKETQEEFFHTFNHLHQSNRQIVLSSDRPPDAIPTL
                     EDRLRTRFVMGLLTDIQPPDVETRLAILRKKAEDEASPVPDEILAFIAENINDNNIRA
                     LEG"
BASE COUNT          339 a          802 c          751 g          305 t
ORIGIN      
        1 caggatctgg gcgctgaact cgaagattcc gtcgaacatc tcggcgtcct atcgggggtg
       61 gtgggaggcg ggctcgggca cgcccggcac ggggtcgtag ccgtggccgc cccagggggc
      121 gcagcggacg atgcggcgca ccgtcagcca ggtcccgcgg ccggcaccgt ggtgggccag
      181 ggcttcgcgg gcgtaggtcg agcaggaggg cacgtagcgg cagggggaca tgcgtccctc
      241 ggtggccttc tggtacagcc agacgaggcg gtcgagggtc cgggcgatca cgaccggacc
      301 ccggtgctcg acgccacggc acgggcgagg tgccggcgga gctcaaccgt cggcagcccg
      361 accacgtcgg ggcgcacacc gacgaggtag ccgccggcgg ggagcacgcc ctcagcgtcg
      421 aggtcgtcca tcagcgggcg cagccggcgg cggacgcggt tgcgcacgac ggcggagccg
      481 accttcttgg acacggcgta ggccacctgc gcgaccgggt gctcagcatc ggtcggcggg
      541 gcgtggacga tccagatcgg gccggacgac ccgcgagcac gcgaccgcga cagggcggcg
      601 aagtcctcgc gggagcggac cggcgcgacc acaccggtca ggccgagagc ttggcgcggc
      661 ccttctggcg ccggttgcgc acgacggcgc gaccggcgcg ggtcgccatg cggtggcgga
      721 agccgtggcg gcgggcacgc cgctgcttct tgggctggta ggtgcgcttc actgggttcc
      781 tcctcgactg cgaccgccgg cgccgcgagg ggccggcccg gaggacggct gcgcggtgga
      841 tgttcgccag gcggtgggcg cgacggcgcg ccggaccaac ccgccacgct accgatggct
      901 cgcgggtggg accaaccggg cggtgcgcgg cgcccatccg ggccggagac atccgtcaca
      961 tcgggccacc cgcgagatcc tcctcgacgc ccttgcaccc gacgttccgg ttgctgctac
     1021 gttgcgaaac cacggcggga acggctcctc cgggctccgg cctggcactg caacccgctc
     1081 gagctaccgg acgccgcccg gacccccggt cgtcgtcttt ttcttttcca catcatgtgg
     1141 actgagttgt ggattcttgg tatcggacag cgggaggcgg ttcgtccatg agcgacgccg
     1201 agaccgtatg gaagggctgc gccgccctcc tccgcgaaca ggtttccggc gtggtgtgga
     1261 gctcgtcgtt ccagaacgtc gagccgctga gctacgacgg gaccacgctg gtcctcggct
     1321 tcccctccac cgtggtgcgt gaccgcgtcc aggagcgcta cctgtcgctg ctgcgcgcca
     1381 acctgtccga gatgggcctc gacgtcgccg tcaccctcga agcgagagcg gccacgcccc
     1441 aggaggtccc gctcttcgac gagctggccg acatccgcgc cgccaccgcc gaggcgccgg
     1501 tcgccgacaa cggccagcgg gccccgagcg gcatcgcgcc gtactccccg gactcctccg
     1561 agggccgctc gacgctgaac gacaagtaca ccttcgacgc cttcgtcacc ggcaactcga
     1621 accgcttcgc ccacgccgcc tcgctccggg tggccgagac gccggcccgg tcgtacaacc
     1681 cgctgttcat ctacggcgac gccggcctcg gcaagaccca cctgctccag gccatcggca
     1741 actacgtgct ggagaactac cccacctaca aggtgcggta cgtgacctcc gagcagttcc
     1801 tgtccgagtt cgtcgactcc atccgcaccg gcaccaacga ggcgttcaag cggcggttcc
     1861 gcgacatcga cgtcctcatg gtcgacgaca tccagttctt cgagggcaag aaggagaccc
     1921 aggaggagtt cttccacacc ttcaaccacc tccaccagtc gaaccgccag atcgtgctga
     1981 gctccgaccg gccgcccgac gccatcccga cgctcgagga ccgcctccgc acgcgattcg
     2041 tcatgggcct gctcaccgac atccagccgc ccgacgtcga gacccgcctg gccatcctgc
     2101 gcaagaaggc cgaggacgag gcctccccgg tccccgacga gatcctcgcc ttcatcgccg
     2161 agaacatcaa cgacaacaac atccgggccc tcgaggg
//
LOCUS       DATISU010000044        18383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_421237, whole genome shotgun sequence.
ACCESSION   DATISU010000044 DATISU010000000
VERSION     DATISU010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 18383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18383
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421237"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(435..1376)
                     /locus_tag="VLR27_02005"
     CDS             complement(435..1376)
                     /locus_tag="VLR27_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HSP02245.1"
                     /translation="MATNTSVVLYDPQETSAEVKALAGFLAGYSGRTREAYTLDLRMF
                     YRWCDEHHLELFTVTRTHIELYARGLEELGRAPATIGRRLSTLVGFYRYAAEEGIIEH
                     SPAVHIRRPRLDYESHAVGLDRNELGAFLVAAGLSSPRDHALCSLLALNGLRISEALG
                     ANIEQLGLERGHRTLVVHRKGGKTVTIPLAPRTARALDLAIGERIDGPLFLGANGERM
                     TRDAAARMVRRVAKAAGITKRIGPHSLRHSFITAALDAGVPLRDVQEAASHADPRTTM
                     RYDRARQSLDRHATYIVATFVAGSSRGDTSGPTAGHR"
     gene            complement(1707..3467)
                     /locus_tag="VLR27_02010"
     CDS             complement(1707..3467)
                     /locus_tag="VLR27_02010"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HSP02246.1"
                     /translation="MSGDALATLAVLVAMFGLLATDRFPPSGVLLGGLAALVLGDVID
                     PEAGFAGFANQAPLTVAALYVVAAGARRTGLLAGITGRVLGGDGGRSSMARLCLPVAG
                     ISSVFNNTPMVAMLLPEVASWARSRRLPVSRFLMPLSFAAILGGTVTLIGTSTNLVVS
                     GVLEQQGYEPFSLFELTPVGLPVAIIGLAVLIGAATSLLPDRSDPALEAVSALREYAL
                     HLEVEPAGALVGTSITDAGLRSLSGVYLADIVRNDRHLGPVSPDELLEAGDLLIFVGD
                     VTDVLDLRARAGLRPPPEQADVLDDAREPLYFEAVIGRESALVGRTLKEVGGRTGYRA
                     AVVAIHRSGSRVEGKLGDVELHAGDTLVLLASSDLRTARRAMEDFLVIAPLQHAVPVI
                     GRKARWVALALAAFVVCAGFELTTVFEAALIAAAVVVGAQVVSFAEAKRSIDLDVIVM
                     IAAALGIGSAVESSGLAQDVADVATDGLGFLGVVGVVLGILVTTSLLTEIVTNNAAAA
                     VVVPIAIRAAEAVDLDLRIMAVGVAVVASSSFLTPIGYQTNAMVYGPGNYRFGDFVRV
                     GGIVNLVVLAAATTMVVALG"
     gene            complement(3527..4573)
                     /locus_tag="VLR27_02015"
     CDS             complement(3527..4573)
                     /locus_tag="VLR27_02015"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HSP02247.1"
                     /translation="MATNPSPADTAEPTTTASAFGATFVDRMPVAEFVDGSWGDPRVD
                     PVQPLALHPAAHVLHYGSACFEGLKAHRGGDGTVRIFRLDRHVARMQRSAEVLVLPVP
                     PTDLLTGMIVGAVRAGLDQVPEPPGSLYLRPILIGVDANIGSAARPSDDALLYVLASP
                     VGDYFAGGLRALVVAVSGQPRTTPQFGVVKTGANYAMALGTTRRANAELGADQVLFAP
                     DGRVEETGASNFLLIDGERIVTPELTDAFLHGVTRDSLLQIGADLGYEVEERPVSVDD
                     LVAWAARPDAEAALSGTAAALSPVGQLVVDGERIAVGTGEVGPNAVRLRDALREVQTG
                     VRADPGGWLTEVRP"
     gene            complement(4590..5246)
                     /locus_tag="VLR27_02020"
     CDS             complement(4590..5246)
                     /locus_tag="VLR27_02020"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSP02248.1"
                     /translation="MSSMYGDEHRELQDRFDSRALADTLAAVTVMPVIDEDAKAFIGS
                     RSFFFLSTVDGDGQPTVSYKGGAPGFVRVVDDTTLLFPSYDGNGMYLSMGNIAGQGRI
                     GLLFIDFETPHRIRVHADATLVAEGELLDSYPGADLVVRATVRQSFVNCGRYIPKRAI
                     PDATHHLPDEQGNVPLAEWKRIDLLQDVLPERDRGRAAAEDGTIDAEEYANKVARGEI
                     "
     gene            complement(5296..7584)
                     /locus_tag="VLR27_02025"
     CDS             complement(5296..7584)
                     /locus_tag="VLR27_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HSP02249.1"
                     /translation="MPEDRHEPQRAPTPADEVVSWLAALGLAVITAVRLLLLLDATVG
                     WFLAETAWPPVRRRAQDVVAQVVGLAQRTGRAAPTWAAAQRTRLALWVGSGEVAGRRA
                     IRGARRSAGAGVAAVGRSRATRRWGRRAVGAGALAAALAGGLVLASDPLLSGVARVAI
                     DEDELDELSHLSQTSVVTAADGATTLGVVERERRRVVELKALPEHVTAMVLAAEDHRF
                     EEHEGYDLTGLVRAAVTNARSGEVEQGGSTITQQLAKLNFTGDDPSIGRKVEELLYAV
                     RLEDELSKEQLLSRYLNQVYFGNGAHGIAAAAEEYFGTAPEDLSPAQAATLAGIIQRP
                     SSLDPRQDPDRVQRRRDVVLRRAAAEGLLTRDEADASTAEPLELAPPAHRPRTDPILD
                     AVRAEVRGIEALGSTPEARLEQLETGGLRVETTLDPTLQHAAFETIATTFPEATGATA
                     AVAAVDPRTGAIRALRGGRAGAGGFDLARQGRRQPGSTFKPLTAVAALEHGLSTEQGL
                     VGDGPIELEHGGPEPWRVDNFEGADLGMVDLRAALRHSVNTAFAQLGVAVGPEAVADV
                     AERVGIDPEAALGDPDQRGPSVALGGVAHGVSPLELASAYTALATEGRWAEPHLVHRI
                     LDADGRVVFERDASPAQVIDPEVAGTVRAMLEGALQEGTGRAARIDDVAAFGKTGTSQ
                     DGADAWFVGSTTDLTTAVWVGHPDGRVAMPEATGGRLAAPIWRAVTSTWTDAHPSGTW
                     PPPDDELESAPGLPLPRPEWVR"
     gene            7667..9037
                     /locus_tag="VLR27_02030"
     CDS             7667..9037
                     /locus_tag="VLR27_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HSP02250.1"
                     /translation="MTEQRVDVVVVGAGFAGLTAARELVGAGRSVVVLEGRDRVGGRT
                     CTEEHLGTWIDLGGQWIGPGQDRIVALVDELGLATYPQPEAGDDVLLDGDDARRVANV
                     AFGFDDDELTSYLELVGALEDMAEQVPLDAPWSAPQALEWDGMSLAEWVRTRGVVERV
                     AGLFEVGVQAVFAASSAQLSLLHAAHYVHSAGGWSELTDSEGGAQQDRVEGGMQPVAE
                     RLAGQLPDRTVRLSHAVRGIAQDADGVRVEADAPDGPVVFGAARVVVAIPPTLTGRIT
                     YDPPLGGQRDQLVQRMPQGSVVKFHVLYEEPWWRDEGLSGMVLAPGEPIGVTFDCTPP
                     AGRPGLISGFFEGPSAVAAGAQTMEERREVVVGVLARAMGERARDVTGYVDRDWSAEP
                     FTRGCYGAHLPPGAWTVYGPALRRPVGRIHWAGTETAERWTGYIDGAIDSGQRVAAEV
                     LAAGGS"
     gene            9045..10280
                     /locus_tag="VLR27_02035"
     CDS             9045..10280
                     /locus_tag="VLR27_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02251.1"
                     /translation="MCDTFCVRRPGGMLFAKSSDRPVDEPQVLEWLHGRAPGGAVRAT
                     HVAIPDAGAGPVLGSRPAWMWGLEHGVNEHRVAIGNEKVWTVDDPRAAPDALLGMDLV
                     RLGLERGATADQAVDVIVELLDAPGQGGSGEEGADEPYWSSFLVIDPDGGWVVETSGR
                     SWVAAPVGAGASISNRLTLSTDWTRSSADVEAGSDWDDRRSPAAPTGIADHRLAATRR
                     CIAAGSAVTPQRAAAALRDHGGGPWGEPGVIGAPTPVPDDVHDDWSGVTVCMHVRDYQ
                     CTTASMVADLPADPGEPLRVWAALGTPCASVYLPIAVLDGEAVVPPVLGDVEAWRSFS
                     ALSRAVEQPGAAGSDALHAIRTSLAPVEAAAWADADELWTARAGADRWRGAASGWDRL
                     VRGAVADVSSTIGAPRPAG"
     gene            complement(10281..12809)
                     /locus_tag="VLR27_02040"
     CDS             complement(10281..12809)
                     /locus_tag="VLR27_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HSP02252.1"
                     /translation="MPPAEHLRRLRRVVAVALVLALTAACTSDGSGDEQADDATETET
                     DTETVTASDAEDRAYYILPPGNYGGLPTTDDSLDQLPLYDGLTPLRGDVTDADIDELF
                     LPMDFEPVGETTPLDVGRDGVTVVYDDYGVPHIEGETREDLAFGAGYVSVRDRGLLLD
                     FGRGPARAAVADVPGIDAFSLVTSAQEFVPSEAAEQLVRDQVDLLVETYGEEGEEIVA
                     EMQAYADGANAYYEDNPDGTERAPFTVEDVVATTAFIGSIFGAGGGGEAANAEFLAAL
                     QNRLGADRGRQAWEDLLQTDDPEAPTTLEERFEYGTFTGGDVVGSVVIDEGSIISLDP
                     REPAAGAAPLVEGEQAAYRVDAAGDPPDRQASNWLTVAPEASAEGTTLGVMGPQLGYF
                     YPEIVMQVHLSGPGIEAQGAAVPGLAMYLLIGRTQDYAWSLTSAGLDVRDVFAEQLCE
                     PDGSAPTRESGSYLFEGECTPFEQFEAGTLGGTPIRYPVSVHGPVIGTATVDGQPVAL
                     TRQRSTFGRDGLNLAALKSMTQGDAGTAEEFWDSASRFGFTFNWGYVNREEIGYFASG
                     RLPVRAEGLDRRLPTWGTGEYEWQGFLEPDQHPHAAGHPDDRLVNWNNQSAPGFMHSD
                     TELYGSVHRVENFDQWPDPIALADVVSVMNRAATEDVRASLWPVISEVLRGGDAPSEL
                     AGSAVDILDAWVSDDAPLLDADEDGSYDEAGALLLDELWPAIERAALQPVFGGVLDAG
                     IALRGIDGESVVDKDLRTLLGQDVDGPFQLSYCGEGDLDACRTDLWTAVDQRLGELAG
                     ERGPDPATWLAEARRSTFVPGLIPDDFRATNRPTYQQLLELAPR"
     gene            12921..13625
                     /gene="ric"
                     /locus_tag="VLR27_02045"
     CDS             12921..13625
                     /gene="ric"
                     /locus_tag="VLR27_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HSP02253.1"
                     /translation="MTTLDASRGQLDRTLGELVAERPARARIFERLGIDYCCHGHRPL
                     VDAAGEAGLDPAAVAAELDAVDDVAGADVDRLEPVALVDHIVDTHHRYLHEELPLLVA
                     LAEKVHGVHGGRHPELARVAALVTEIHDDLVPHLAKEEQVLFPAIRAWADGQQSFPFG
                     TLSNPVRMLMTEHDRAGELLEDVRAVTDGYQPPADGCASYVSLYERLEHLELDTHRHI
                     HLENNVLFPAVTPDGS"
     gene            13639..13986
                     /locus_tag="VLR27_02050"
     CDS             13639..13986
                     /locus_tag="VLR27_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02254.1"
                     /translation="MTDPPRQLDLLDAARAVQRAATDGDMDTLHGEVCRLRNALVEHV
                     AGCDLGDDGGVQRRLARHGQQRLLHFIDELLSTTHESAESCTCLVRGAELRSLLIRQL
                     RLEAVASTSEVVR"
     gene            complement(13983..14420)
                     /locus_tag="VLR27_02055"
     CDS             complement(13983..14420)
                     /locus_tag="VLR27_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSP02255.1"
                     /translation="MCDYCDCRTRPLLAHLGADHERILTGVHGLREASEPGDADRRMA
                     LAAELVDVLEEHGRLEEAALYPELALAGVPTDAMEAEHGEVHAVLRTAAQASEVDPAA
                     LETALRRLEAHIHREEYDLFPAAHQVLDDAAWDRIHLELEASS"
     gene            complement(14497..15729)
                     /locus_tag="VLR27_02060"
     CDS             complement(14497..15729)
                     /locus_tag="VLR27_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HSP02256.1"
                     /translation="MHENFPHIDRHLGALIALVGVAGGLVGAAYISALHVLEASLAPE
                     HFASWHTQLLVLVGVGLAIGALIKALGDPGDVELLVDNIHVSGGSSDVRSLRSLLPAS
                     LLGIAAGSALGPEAPLVQTAGTIGALAGRSRALTTEQVRVLAICGMASAFAVLFGAPL
                     GSAVFALEILHRRGLEYYEALLPALGGALIGFAVFIGLEGVGYGPLIDLPAAVDVSTS
                     DLLWGGVAAVVGSALAAIFTLLVGALRRLAVVVPGLLRPAAGGAVLGVLAIWSSAALT
                     FGEAQISVLGMGTWSASTLAAALVAKLVASAVCLATGWRGGFIIPLFFVGATAGQLIH
                     IAVPSVDATVLMAACMVATNVGVTKTPVGSTLVIAKMAGLQLLPSTTLAAVGAMLLTN
                     QVNLLASQRRRAPSTAAT"
     gene            15776..16195
                     /locus_tag="VLR27_02065"
     CDS             15776..16195
                     /locus_tag="VLR27_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSP02257.1"
                     /translation="MADDEDEALGTRDYRTLARFRRELRAFLHFSEEAAGAEGLTPAQ
                     HQLLLAVRGHPADGPPSISEVAEALRLKTHSVGGLVDRAEEAGLVVSHADPADGRRRL
                     IEVTPAGEEILERLSALHRRELRRFRDEMGALLDDLD"
     gene            complement(16192..16632)
                     /locus_tag="VLR27_02070"
     CDS             complement(16192..16632)
                     /locus_tag="VLR27_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HSP02258.1"
                     /translation="MRDTPRPRPTEDLDSVEEIAEMVRRFYADVAMDDLLGPVFEDVA
                     EVDWSEHLPKLTAFWARALIGEPGYAGNPFRSHAEIHARAPFTPAHFRRWLTLFHETI
                     ELGWVGPRADRARELADAVARVHSEQLVGEAVALDHPVTAPTSR"
     gene            16705..16971
                     /locus_tag="VLR27_02075"
     CDS             16705..16971
                     /locus_tag="VLR27_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02259.1"
                     /translation="MTEIVQTIERITAESAALRGALIGTVIGFFAVGGFTFWVALNAG
                     TSTVAAVGLALFAGFWGGPGFGGMLGATLAVTRNEEREREAAAA"
     gene            16968..>18383
                     /gene="sufB"
                     /locus_tag="VLR27_02080"
     CDS             16968..>18383
                     /gene="sufB"
                     /locus_tag="VLR27_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HSP02260.1"
                     /translation="MTDIVQELVERKYEFGFHSDIDTELAPRGLNEDVVRLISAKKDE
                     PEWLLEWRLAAYRHWTTMEPPVWQNVQHPPIDFQDMHYWAAPKPKGPAPASLDDVDPE
                     IRATFDKLGISLAEQERLSGVAVDAIIDSVSVATTFKATLAEAGVIFCSFSEAVREHP
                     ELVRRYLGSVVPPRDNFYAALNSAVFSDGSFCFVPAGVRCPMELSTYFRMNAAETGQF
                     ERTLIVAEEGATVSYLEGCTAPMRDENQLHAAVVEIAAMDDSDVKYSTVQNWYPGDEN
                     GVGGIYNFVTKRGRCGHRAKISWTQVETGSAITWKYPSVILQGDDSVGEFYSVAVTTG
                     RQQADTGTKMIHIGKNSRSTIVSKGISAGKAQQTYRGLVKVLRSATGARNHTQCDSLL
                     IGSECGAHTFPYIEVKNDSAQVEHEASTSKISDDQLFYCRQRGLDEEDATSMIVNGFC
                     REVFDELPMEYAVEAHALMRLS"
BASE COUNT         2501 a         6445 c         6981 g         2456 t
ORIGIN      
        1 agcgctggag ctcgaggcct atctgttcgg tgcgctcgtc gcaccacccc atgaacacga
       61 cctggtcgcc gtggcgctca acgagcgctt ctcggaactc ggtggggatc acccggttcc
      121 gtacgagggt gatgcgccgc aacggtgacc gcccaccagc tcgccggtgg gggtgaccac
      181 gtcgcccggg ccacgattct tgtcgtgaac ctgccccccg gccagaagga gcgaccgaag
      241 gtgactccgc ccgccagcac agcggtcggg ctggccagta cagaacgccc cttcaagtgc
      301 ggtgcgccgt ctcgcaggcc gcgctggtaa ttgccggttc ttcttgcgtc aagtcgagcg
      361 cagctccggt cgctgaccgc tccgagatgg ggatacggag cgccccacct tggagcgctc
      421 cgttcccggt caggtcagcg gtgacccgca gtcggtccgg aggtgtcgcc cctgcttgag
      481 ccggcgacga aggtcgccac gatgtaggtg gcgtgtcgat cgagggactg gcgggcccgg
      541 tcgtatcgca tggtcgtcct gggatcggcg tgcgacgctg cctcctgcac gtcccgtagc
      601 ggcacgccgg cgtcgagcgc tgcggtgatg aaggaatggc gcaggctgtg cgggccgatc
      661 cgcttggtga tcccggccgc cttggccacc cggcgcacca tccgtgccgc ggcgtctcgg
      721 gtcatgcgct ccccgttggc gccgaggaac agcggtccgt cgatgcgttc gccgatggcg
      781 aggtcgagtg cccgtgcggt ccggggtgcg agcgggatag tgacggtctt gccgcccttg
      841 cggtgcacga ccagggtccg gtgaccgcgc tcgaggccga gctgttcgat gttggcgccg
      901 agggcttcgg agatccgcag cccgttgagg gccagcagcg agcacagggc atggtcccgg
      961 ggtgaggaga gaccggcggc gacgaggaag gctccgagct cgttgcggtc caggccgacg
     1021 gcgtgggact cgtagtcgag ccggggccgg cggatgtgga cggccggtga gtgctcgatg
     1081 atgccctcct cggccgcgta gcggtagaag ccgaccagcg tcgacagccg ccggccgatt
     1141 gtggccgggg cccggccgag ctcctcgagc ccgcgggcgt agagctcgat gtgggtgcgg
     1201 gtgacggtga acagctcgag gtggtgctcg tcgcaccacc ggtagaacat gcgcaggtcg
     1261 agggtgtatg cctcgcgggt tcggccggag tagccggcta ggaagccggc gagggccttc
     1321 acctcggcgg aggtctcttg cgggtcgtag aggacgaccg atgtgttcgt ggccatgacg
     1381 gctccttgcg atgggagccg ggccgagcca ggcggtccgc gcccagctcg ttgatgtggc
     1441 gcacctatca ccgcgcagat cggacgaact cgccaccgtc ccgggcggcg ccggcggcgc
     1501 aacctcgagt ccaggcgctc ggctccgtcg cctaccggcg tcggagagct ggcgttcccg
     1561 cgggcggtcc gctctcgaag tcgctctgcc ggccggcaac cgacgtgccg gtgctcgttc
     1621 gacttccgga gcagtagcca cgaggaccgc cactcggcgg tcctcgtgtg gaacccgacc
     1681 ggggcggagc cgtgcggtga gcggcctcag cccagcgcga cgaccatcgt ggtggcggcg
     1741 gccaggacga cgaggttcac gatgcccccg acccgtacga agtccccgaa gcggtagttg
     1801 ccggggccgt agaccatggc gttggtctgg tagccgatcg gggtgaggaa cgacgacgac
     1861 gccaccaccg ccacccccac cgccatgatc cgcaggtcca ggtcgaccgc ctcggcggcg
     1921 cggatggcga tgggcacgac gacggcggcc gcggcgttgt tggtgacgat ctcggtcagc
     1981 aggctcgtcg tcaccaggat gccgagcacg acgccgacca cgccgaggaa gccgagcccg
     2041 tcggtggcga cgtcggcgac gtcctgggcc agcccgctcg actcgaccgc ggagccgatg
     2101 ccgagggcgg cggcgatcat gacgatcacg tcgaggtcga tggagcgctt ggcctcggcg
     2161 aagctgacca cctgagcgcc cacgacgacg gcggcggcga tgagggcggc ctcgaagacg
     2221 gtcgtgagct cgaagccggc gcacacgacg aaggcggcga gggccagcgc cacccaccgg
     2281 gccttgcgac cgatcaccgg cacggcgtgc tggagcgggg cgatgacgag gaagtcctcc
     2341 atcgcccgcc gggccgtgcg caggtcgctg ctggccagca gcacgagggt gtcgcccgcg
     2401 tggagctcga cgtcgccgag cttgccctcc acgcggctcc cggagcggtg gatggccacc
     2461 acggcggccc ggtagccggt gcgcccgccg acctccttga gcgtgcggcc gaccagcgcc
     2521 gactcccgac cgatgacggc ctcgaagtac agcggctccc gcgcgtcgtc gagcacgtcg
     2581 gcctgctcgg gcgggggtcg gaggccggct cgggcccgca ggtcgagcac gtcggtgacg
     2641 tcgccgacga agatcagcag gtccccggct tccagcagct cgtcggggct caccggaccg
     2701 aggtggcggt cgttgcggac gatgtcggcg aggtacacgc cggacaggct gcggaggccg
     2761 gcgtcggtga tcgaggtgcc gacgagggcg ccggcgggct cgacctcgag gtggagggcg
     2821 tactcgcgca gggcggagac cgcttcgagg gccgggtcgg agcgatcggg caggaggctg
     2881 gtcgcggcgc cgatcagcac tgcgaggccg atgatcgcga ccgggaggcc gacgggggtg
     2941 agctcgaaca ggctgaacgg ctcgtagccc tgctgctcga gcactccgga caccacgagg
     3001 ttcgtcgacg tcccgatgag ggtcacggtg ccgccgagga tggcggcgaa ggacagcggc
     3061 atgaggaagc gcgacaccgg cagacgcctg ctgcgggccc agctggccac ctccggcagc
     3121 agcatggcga ccatcggcgt gttgttgaac accgacgaga tgccggcgac cgggaggcac
     3181 aggcgggcca tggacgaccg gccgccgtcg ccgccgagca cccgaccggt gatgccggcc
     3241 agcagacccg tccggcgggc accggcggcg accacgtaca gggcggcgac cgtcagcggg
     3301 gcctggttgg cgaacccggc gaacccggcc tcggggtcga tgacgtcacc gaggaccagg
     3361 gcggcgaggc cgccgagcag cacgccggac ggggggaagc ggtcggtggc cagcaggccg
     3421 aacatggcga ccaggacggc caaggtcgcc agcgcgtctc cggacatcgg cggcaccgta
     3481 ctggtcgtgg atcggggcgc cggcagccgg cgccggcctc agccgctcag ggccggacct
     3541 cggtgagcca accgcccgga tcggcgcgga cgccggtctg cacctcgcgc agcgcgtccc
     3601 ggagccggac cgcgttgggt ccgacctcgc cggtcccgac ggcgatccgc tcgccgtcga
     3661 ccacgagctg gccgaccggc gagagcgcgg cggcggtgcc ggagagcgcg gcctcggcat
     3721 cgggtcgggc ggcccaggcc accaggtcgt cgacgctgac cggccgctcc tcgacctcgt
     3781 agccgaggtc ggcgccgatc tgcagcagcg agtcacgcgt caccccgtgg aggaacgcat
     3841 cggtgagctc gggcgtcacg atgcgctcac cgtcgatgag gaggaagttc gatgcccccg
     3901 tctcctcgac ccgaccgtcg ggcgcgaaca gcacctggtc cgcaccgagc tcggcgttgg
     3961 cccgcctcgt cgtgccgagc gccatcgcgt agttggcgcc ggtcttgacc acgccgaact
     4021 gaggcgtcgt gcggggctgt ccggaaacgg cgaccacgag tgctcgcagg ccgccggcga
     4081 agtagtcgcc gaccgggctg gccagcacgt agagcagggc atcgtcgctc gggcgggcag
     4141 ccgagccgat gttggcgtcg acgccgatga ggatcggccg caggtacagc gagccgggcg
     4201 gctcgggcac ctggtcgagc ccggcgcgga cggcgccgac gatcatcccg gtgagcaggt
     4261 ccgtcggcgg gaccggcagc acgagcacct cggcggagcg ctgcatgcgg gccacgtgcc
     4321 ggtcgaggcg gaagatccgc accgtcccgt cgcctccccg gtgggccttc aacccctcga
     4381 agcaggcgct cccgtagtgc aggacgtggg cagcggggtg cagcgccagc ggctggaccg
     4441 ggtcgacgcg ggggtcgccc cagctgccgt cgacgaactc cgccaccggc atccggtcga
     4501 cgaaggtggc cccgaacgcc gacgcggtgg tggtcggctc ggcggtgtcg gcaggggatg
     4561 ggttcgtggc catggcagca gtgtcgcggt cagatctcgc cccgggcgac cttgttcgcg
     4621 tactcctcgg catcgatggt cccgtcctcg gcggcggccc gaccgcgatc gcgctcgggc
     4681 agcacgtcct gcaggaggtc gatgcgcttc cactcggcca gcggcacgtt gccctgctcg
     4741 tcggggaggt ggtgggtggc gtcggggatg gcccgcttcg ggatgtaccg gccgcagttc
     4801 acgaacgact gcctgaccgt cgcccggacc acgaggtcgg cccccgggta gctgtcgagg
     4861 agctcgccct cggcgaccag ggtggcgtcg gcgtggaccc ggatgcggtg cggggtctcg
     4921 aagtcgatga acagcagccc gatgcggccc tgcccggcga tgttccccat ggagaggtac
     4981 atgccgttgc cgtcgtagct cgggaacagg agggtggtgt cgtcgacgac ccggacgaag
     5041 cccggcgcgc cgcccttgta cgacaccgtc ggctggccgt cgccgtcgac cgtgctgagg
     5101 aagaagaacg agcgggaccc gatgaacgcc ttcgcgtcct cgtcgatcac cggcatgacc
     5161 gtgaccgccg ccagcgtgtc cgccagggcg cggctgtcga agcggtcctg gagctcccgg
     5221 tgctcgtctc cgtacatgct ggacatggct gctcccccgg atcgtggtcg gccgccagtc
     5281 tggctgcccg gttcgtcagc ggacccactc gggacggggg aggggcaggc ccggggcgga
     5341 ctccagctcg tcgtccggcg gcggccacgt cccggagggg tgcgcatcgg tccacgtgct
     5401 ggtgaccgcg cgccagatcg gcgcggcgag gcggccgccg gtggcctccg gcatcgccac
     5461 ccgcccgtcg gggtggccca cccacaccgc ggtcgtcagg tcggtcgtgg agccgacgaa
     5521 ccaggcgtcg gcgccgtcct gggacgtccc ggtcttgccg aaggcggcga cgtcgtcgat
     5581 gcgggcggcc cgaccggtgc cctcctggag cgcgccctcg agcatggcgc ggaccgtgcc
     5641 ggcgacctcg gggtcgatga cctgggcggg tgatgcgtcc cgctcgaaga ccacccggcc
     5701 gtcggcgtcg aggatgcggt ggaccaggtg cggttcggcc catcgcccct cggtcgcgag
     5761 cgcggtgtag gcgctggcca gctcgagcgg ggacacgccg tgggcgacgc caccgagcgc
     5821 gaccgacggg ccccgctggt cggggtcacc gagcgcggcc tcgggatcga tgccgacccg
     5881 ctcggcgacg tcggccacgg cttcggggcc gacggcgacg ccgagctggg cgaaggcggt
     5941 gttcacgctg tggcggaggg cggcgcggag gtcgaccatc ccgagatcgg cgccctcgaa
     6001 gttgtcgacc cgccacggct cgggaccgcc gtgctcgagc tcgatcggcc cgtcgccgac
     6061 caggccctgc tcggtcgaca agccgtgctc gagggcggcg accgcggtca gcggcttgaa
     6121 cgtgctgccc ggctggcggc gcccctggcg ggcgaggtcg aacccgccgg cgccggcgcg
     6181 gccgccccgc agggcccgga tcgcgcccgt gcgcgggtcg acggcggcga cggccgcggt
     6241 cgcacccgtg gcctcgggaa aggtggtggc gatggtctcg aacgcggcgt gctgcagcgt
     6301 cgggtcgagg gtcgtctcga cgcggagccc gccggtctcg agctgctcca accgcgcctc
     6361 gggcgtcgag ccgagcgcct cgatgccccg cacctcggcg cgcacggcgt cgaggatcgg
     6421 gtcggtgcgc ggtcggtggg ccggcggtgc cagctcgagg ggctccgcgg tcgaggcgtc
     6481 ggcctcgtcg cgggtgagca ggccctccgc cgcggcccgt cgcaggacga cgtcgcgccg
     6541 gcgctggacg cggtcggggt cctggcgcgg gtcgagcgag ctgggtcgct ggatgatgcc
     6601 ggccagcgtg gcggcctggg ccgggctgag gtcctcgggg gcggtgccga agtactcctc
     6661 ggccgccgcg gcgatgccgt gggcgccgtt gccgaagtac acctggttca ggtagcggga
     6721 cagcagctgc tccttcgaga gctcgtcctc gaggcgcacg gcgtagagca gctcctcgac
     6781 cttgcggccg atcgaggggt cgtcgcccgt gaagttcagc ttggcgagct gctgggtgat
     6841 ggtggagccg ccctgctcga cctcgcccga gcgggcgttg gtgaccgcgg cccggaccag
     6901 gcccgtcagg tcgtagccct cgtgctcctc gaagcggtgg tcctcggcgg cgagcaccat
     6961 ggcggtcacg tgctccggga gggccttgag ctcgacgacg cgccggcgct cgcgctccac
     7021 cacgccgagc gtggtggccc cgtcggcggc ggtcaccacc gacgtctggg agaggtgcga
     7081 cagctcgtcg agctcgtctt cgtcgatcgc cacgcgggcc acgccgctga gcagggggtc
     7141 ggacgccagg acgagcccac ccgccagcgc agcggcgagg gcgccggctc ccacggcccg
     7201 acgcccccag cgtcgggtgg cccgggaccg gccgaccgcg gcgacgccgg cacccgcgct
     7261 cctccgagcg ccccggatgg cgcgtcgccc ggccacctcg cccgatccca cccagagcgc
     7321 gagccgggtc cgctgcgccg ccgcccacgt cggcgccgcc cgtccggttc gctgggccag
     7381 gccgacgacc tgtgcgacca cgtcctgggc tctccggcgc acgggaggcc aggccgtttc
     7441 ggcgaggaac cacccgacgg tcgcgtccag gaggagcagc agccgcacgg cggtgatcac
     7501 cgccaagccc agcgccgcca gccaggagac gacctcgtca gcaggggtgg gggcgcgctg
     7561 aggctcgtgt ctgtcctccg gcacggagag cgccttaccc ggccgggacc ggcggctaga
     7621 ccccccactg caccggggcg ggaccggccg gtagcgtcgc ccggcgatga ccgagcagcg
     7681 tgtggatgtc gtcgtcgtcg gagccgggtt cgccggcctg acggccgccc gggagctggt
     7741 cggcgccggc cggtcggtgg tcgtgctcga ggggcgggac cgcgtcggtg ggcgcacctg
     7801 caccgaggag cacctgggca cgtggatcga cctcggtggg cagtggatcg ggccgggcca
     7861 ggaccgcatc gtcgcgctcg tggacgagct cggcctggcc acctacccgc agcccgaggc
     7921 cggtgacgac gtcctcctcg acggtgacga cgcccgacgg gtggcgaacg tggccttcgg
     7981 cttcgacgac gacgagctca cgtcgtacct ggagctggtc ggcgccctcg aggacatggc
     8041 cgagcaggtg ccgctcgacg cgccgtggtc cgccccccag gccctcgagt gggacggcat
     8101 gagcttggcc gagtgggtcc ggacccgcgg cgtggtggag cgcgtcgccg ggctgttcga
     8161 ggtcggggtg caggccgtgt tcgcggcctc cagcgcccag ctgtcgctgc tccacgccgc
     8221 ccactacgtc cactccgccg gcggctggtc ggagctcacc gacagcgagg gcggtgccca
     8281 gcaggaccgg gtcgagggcg gcatgcagcc ggtggccgag cggctggccg gccagctccc
     8341 cgaccggacc gtgcgcctgt cccacgccgt gcggggcatc gcccaggacg ccgatggcgt
     8401 ccgcgtcgag gccgacgcac ccgacgggcc ggtcgtgttc ggcgccgcac gagtcgtcgt
     8461 ggccatcccc ccgaccctca ccgggcggat cacctacgac cccccgctcg gcgggcagcg
     8521 ggaccagctg gtgcagcgca tgccccaggg ctcggtcgtg aagttccacg tgctctacga
     8581 agaaccgtgg tggcgcgacg aggggctctc gggcatggtc ctggccccgg gtgaaccgat
     8641 cggggtcacc ttcgactgca ccccgcccgc cggtcggccc ggcctcatca gcgggttctt
     8701 cgagggcccc tcggcggtgg ccgccggtgc gcagacgatg gaggagcgcc gggaggtcgt
     8761 ggtcggggtg ctcgcccggg cgatggggga gcgcgcccgg gacgtcaccg gctacgtcga
     8821 ccgcgactgg tcggccgagc ccttcacgcg ggggtgctac ggcgcgcacc tccccccggg
     8881 cgcgtggacc gtgtacggcc cggcgctgcg ccggccggtg ggacgcatcc actgggccgg
     8941 caccgagacg gccgagcgct ggaccggcta catcgacggg gccatcgaca gcggccagcg
     9001 tgtcgccgcc gaggtcctcg ccgcgggcgg gagctgatcg cgccgtgtgc gacaccttct
     9061 gcgtccggcg cccaggcggg atgctgttcg cgaagtcgag cgaccggccc gtcgacgaac
     9121 cccaggtgct cgagtggctc cacggccgcg cgccgggggg cgcggtgcgc gccacgcacg
     9181 tggcgatccc cgatgccggt gccggccccg tcctgggctc ccggcccgcg tggatgtggg
     9241 ggctcgagca cggcgtcaac gagcaccggg tggcgatcgg caacgagaag gtgtggacgg
     9301 tcgatgaccc gcgcgccgcg ccggatgccc tgctcggcat ggacctcgtc cggctcggcc
     9361 tggagcgggg cgccacggcc gaccaggcgg tggacgtgat cgtcgagctc ctcgacgcgc
     9421 ccggccaggg cggcagcggc gaggagggag cggacgagcc gtactggtcc tcgttcctgg
     9481 tcatcgaccc cgacggtggc tgggtggtgg agacgtcggg ccggtcctgg gtggctgccc
     9541 cggtcggtgc gggcgcctcc atctccaacc ggctcacgct gtccaccgac tggacccggt
     9601 cctccgccga cgtcgaggcc ggctccgact gggacgatcg gcggtcgccg gcggcgccca
     9661 ccggcatcgc cgaccaccgc ctcgccgcca cccggcgctg catcgccgcc ggcagcgccg
     9721 tgaccccgca gcgcgcggcg gccgcgctgc gcgaccacgg cggcggtccc tggggggagc
     9781 cgggcgtcat cggcgcgccg acaccggtcc cggacgacgt gcacgacgac tggtccggcg
     9841 tgaccgtctg catgcacgtg cgcgactacc agtgcaccac cgcgtcgatg gtggccgacc
     9901 tgccggccga cccgggcgag ccgttgcggg tgtgggcggc gctggggacc ccgtgcgcca
     9961 gcgtgtacct gccgatcgcc gtgctcgacg gcgaggccgt ggtgcccccg gtcctcggcg
    10021 acgtcgaggc gtggcggtcc ttcagcgcct tgagccgagc cgtcgagcag cccggcgccg
    10081 cgggcagcga cgccctccac gccatccgga cgtcgctggc ccccgtggag gccgcggcgt
    10141 gggccgacgc ggacgagctg tggacggccc gagccggtgc cgaccggtgg cggggcgcgg
    10201 cgagcggctg ggaccggctg gtccggggcg ccgtggcgga cgtgtcgtcc accatcgggg
    10261 cgccccggcc ggccggttga tcagcggggc gccagctcca gcagctgctg gtaggtcggg
    10321 cggttggtgg cccggaagtc atcggggatg agccccggca cgaaggtgga gcggcgggcc
    10381 tcggccagcc acgtcgccgg gtcgggtccg cgctcgccgg ccagctcgcc cagccgctgg
    10441 tcgacggcgg tccacaggtc ggtccggcag gcgtcgaggt caccctcgcc gcagtaggag
    10501 agctggaagg gcccgtccac gtcctggccg agcagcgtgc gcaggtcctt gtcgacgacg
    10561 ctctcgccgt cgatcccccg cagtgcgatc ccggcgtcga ggacgccgcc gaacacgggc
    10621 tggagcgcgg cccgctcgat ggccggccag agctcgtcca gcaggagcgc gccggcctcg
    10681 tcgtagctgc cgtcctcgtc ggcgtcgagc agcggggcgt cgtcggagac ccaggcgtcg
    10741 aggatgtcga ccgccgaacc ggccagctcc gaaggtgcgt cgccgccccg cagcacctcg
    10801 ctgatgaccg gccacaagct ggcgcggacg tcctcggtgg cggcccggtt catcaccgac
    10861 accacgtcgg ccagggcgat cggatcgggc cactggtcga agttctccac ccggtggacc
    10921 gagccgtaga gctcggtgtc gctgtgcatg aagcccgggg cggactggtt gttccagttc
    10981 acgaggcggt catcggggtg gccggcggcg tgggggtgct ggtcgggctc gaggaagccc
    11041 tgccactcgt actcgccggt gccccaggtc ggcaggcggc ggtcgaggcc ctcggcccgc
    11101 acgggcaggc ggcccgaggc gaagtagccg atctcctcgc ggttcacgta gccccagttg
    11161 aaggtgaacc cgaaccggct ggccgagtcc cagaactcct cggcggtgcc ggcgtcgccc
    11221 tgggtcatgg acttcagggc ggcgaggttc aggccgtcgc ggccgaaggt cgatcgctgc
    11281 cgggtgagcg ccaccggctg gccgtcgacg gttgccgtgc cgatcaccgg gccgtgcacc
    11341 gacacggggt accggatcgg ggtgccgccg agggtgccgg cctcgaactg ctcgaacggg
    11401 gtgcactcgc cctcgaacag gtaggacccg gattcccggg tgggtgccga gccgtcgggt
    11461 tcgcacagct gctcggcgaa cacatcgcgc acgtcgaggc cggcggaggt gaggctccac
    11521 gcgtagtcct gcgtgcggcc gatgagcagg tacatggcca gcccggggac ggcggcgccc
    11581 tgggcctcga tgcccgggcc gctcaggtgc acctgcatca cgatctcggg gtagaagtag
    11641 ccgagctggg ggcccatcac gccgagcgtg gtgccctcgg ccgacgcctc cggagccacg
    11701 gtgagccagt tcgacgcctg gcggtcgggc ggatcgccgg cggcatcgac ccggtaggcg
    11761 gcctgctcgc cttcgacgag cggtgccgcg cccgcggccg gttcacgggg gtcgagcgag
    11821 atgatcgagc cctcgtcgat cacgaccgac ccgaccacgt cgccgccggt gaaggtgccg
    11881 tactcgaagc gctcctcgag cgtcgtcggc gcctcggggt cgtcggtctg gaggaggtcc
    11941 tcccacgcct ggcgcccccg gtccgcaccg agtcggttct ggagcgcggc gaggaactcg
    12001 gcgttggccg cttcgccgcc gccgccggca ccgaagatcg agccgatgaa cgccgtggtc
    12061 gccaccacgt cctcgaccgt gaacggcgcc cgctcggtgc cgtcggggtt gtcctcgtag
    12121 taggcgttgg ccccgtcggc gtaggcctgc atctcggcga cgatctcctc gccctcctcg
    12181 ccgtaggtct ccaccagcag gtcgacctgg tcgcgcacca gctgctcggc agcctcgctg
    12241 ggcacgaact cctgggcgct ggtcaccagc gagaaggcat cgatgcccgg cacgtcggcc
    12301 acggcggcgc gcgccgggcc gcgcccgaag tcgaggagca ggccgcggtc gcgcacgctc
    12361 acgtacccgg cgccgaaggc caggtcctca cgggtctcgc cctcgatgtg ggggacgccg
    12421 tagtcgtcgt acacgaccgt gacgccatcc cggccgacgt cgagcggggt ggtctcgccg
    12481 acgggttcga agtccatggg gaggaagagc tcgtcgatgt cggcgtcggt cacgtcgccc
    12541 cgcagcgggg tcagcccgtc gtagagcggg agctggtcca gcgagtcgtc cgtcgtcggc
    12601 agcccgccgt agttgccggg cgggaggatg tagtaggcgc ggtcctcggc gtcggacgcg
    12661 gtgaccgtct cggtgtcggt ctcggtctcg gtggcgtcgt cggcctgctc gtcgccggac
    12721 ccgtcggagg tgcaggcggc ggtgagggcg aggacgaggg cgacggcgac gacgcgtcgc
    12781 agccggcgga ggtgttcggc ggggggcacg agccgaccat acgcgtcggg acctttgacc
    12841 cttcggcgca gcggcgaccg cccgtagtat tatttctgtc agtcgctaga agaacggcac
    12901 ggccgttcgg aaggagcacc atgaccaccc tcgacgcctc acggggtcag ctcgatcgca
    12961 cgctcgggga gctggtggct gagcgcccgg cccgggcccg gatcttcgag cggctcggca
    13021 tcgactactg ctgccacggg caccgcccgc tggtcgacgc cgccggcgag gccgggctgg
    13081 accccgccgc cgtggccgcg gagctcgacg ccgtcgacga tgtcgctggt gccgacgtgg
    13141 accggctgga gccggtggcc ctcgtcgacc acatcgtgga cacccaccac cgctacctgc
    13201 acgaggagct gccgctcctg gtcgcgctgg cggagaaggt gcacggcgtc cacggcggac
    13261 gccacccgga gctggcgcgg gtcgccgccc tcgtcaccga gatccacgac gacctcgtcc
    13321 cccacctcgc caaggaggag caggtcctct tccccgccat ccgggcgtgg gccgacggcc
    13381 agcagtcgtt ccccttcggg accctgtcga acccggtccg catgctcatg accgagcacg
    13441 accgggccgg ggagctgctg gaggacgtgc gggcggtgac cgacggctac cagccgcccg
    13501 ccgacggctg cgcaagctac gtctcgctgt acgagcggct cgagcacctg gagctggaca
    13561 cgcaccggca catccacctc gagaacaacg tgctgttccc ggcggtcacg cccgacgggt
    13621 cgtgacccgc gtcgggccat gaccgaccca ccccgccagc tggacctgct cgacgcggcg
    13681 cgcgccgtgc agcgggccgc gaccgacggc gacatggaca ccctccacgg cgaggtgtgc
    13741 cgcctgcgga acgcgctcgt cgagcacgtg gccggctgcg acctgggtga tgacgggggc
    13801 gtgcagcgac ggttggcgcg gcacgggcag cagcggctcc tgcacttcat cgacgagctg
    13861 ctctcgacca cccacgagag cgccgagagc tgcacgtgcc tggtccgcgg cgccgagctg
    13921 cggtcgttgc tgatccgcca gctccggctg gaggccgtgg cgtcgacgag cgaggtggtc
    13981 cgctaggagg aggcctcgag ctcgaggtgg atccggtccc acgcggcatc gtccagcacc
    14041 tggtgggcgg ccgggaacag gtcgtactcc tcccggtgga tgtgggcctc gagccgccgg
    14101 agggcggtct ccagcgccgc cgggtcgacc tcggatgcct gcgcggcggt ccgcaggacg
    14161 gcgtggacct cgccgtgctc ggcctccatg gcgtcggtcg ggaccccggc gagggccagc
    14221 tcggggtaga gcgccgcctc ctcgagccgg ccgtgctcct cgagcacgtc gaccagctcg
    14281 gcggcgagcg ccatgcggcg gtcggcgtcc cccggttcgc tcgcctcccg gagcccgtgg
    14341 accccggtga ggatgcgctc gtggtcagcg ccgaggtggg cgagcagcgg tctggtccgg
    14401 caatcgcagt agtcgcacac cggagccacc tcctcgtcga cgtcccggca cccccagcat
    14461 gccgggacgg gtggcggctc cgtcgggtgc aggccgtcag gtggccgcgg tgctcggcgc
    14521 ccgccgcctc tggctcgcga gcaggttcac ctggttggtc aggagcatgg ccccgacggc
    14581 ggccagcgtc gtcgagggga gcagctggag cccggccatc ttggcgatga cgagcgtgct
    14641 gccgaccggg gtcttggtga cgccgacgtt cgtcgccacc atgcatgcgg ccatcagcac
    14701 cgtggcgtcg acggacggga cggcgatgtg gatcagctgg ccggcggtcg ctccgacgaa
    14761 gaagagcggg atgatgaacc cgccccgcca gccggtggcg aggcacacgg cgctggccac
    14821 gagcttggcg acgagggcgg cggcgagcgt ggacgccgac cacgtgccca tgccgaggac
    14881 ggagatctgg gcctcaccga aggtcaaggc agccgaggac cagatggcga ggacgccgag
    14941 caccgcgccg cctgcggccg gtcgcaggag cccggggacg accacggcca gacggcggag
    15001 ggcgccgacc agcagcgtga agatcgccgc caacgccgaa cccacgaccg cggcgacgcc
    15061 gccccagagc aggtcggacg tggagacgtc gactgcagcg gggaggtcga tgaggggccc
    15121 gtagccgacg ccctcgagtc cgatgaacac ggcgaagccg atcagcgcgc ccccgagcgc
    15181 gggcaggagc gcctcgtagt actccaggcc gcgacggtgg aggatctcga gcgcgaagac
    15241 cgccgatccg agcggcgcgc cgaacaggac tgcgaacgcc gacgccatgc cgcagatcgc
    15301 caggacgcgc acctgctccg tggtcagggc tcggctccgg ccggcgagcg cgccgatcgt
    15361 gccggcggtc tgcacgaggg gcgcctccgg tccgagcgcg ctgcccgcgg cgatgccgag
    15421 cagcgaggcc ggcaggagcg agcggagcga ccgcacgtcg ctgctgccgc cggagacgtg
    15481 gatgttgtcg acgagcagct cgacgtcgcc ggggtcgccc agcgccttga tcagcgcacc
    15541 gatggcgagg ccgaccccca ccaggacgag gagctgggtg tgccagctgg cgaagtgctc
    15601 gggggcgagg gaggcctcga gcacgtggag agcgctgatg taggcggcac cgaccaggcc
    15661 gccggcgacc ccgaccagcg cgatcagcgc gccgaggtgc cggtcgatgt gggggaagtt
    15721 ctcgtgcacc gaaacatcgt agagcgatgt attcgcggtg acgtagcgtg gcggggtggc
    15781 cgacgatgaa gacgaggcgc tcggcacccg ggactaccgg accctcgccc gcttccggcg
    15841 cgagctgcgg gccttcctgc acttctccga ggaggcggcg ggggccgagg gcctgacccc
    15901 ggcgcagcac cagctgctcc tggccgtgcg tggccatccc gccgacggtc cgccctccat
    15961 cagcgaggtg gccgaggcgc tgcggctgaa gacccactcg gtggggggcc tggtcgatcg
    16021 cgccgaggag gccggcctcg tcgtgagcca cgccgacccg gcggacggac ggcgccggct
    16081 gatcgaggtg acgcccgcgg gcgaggagat cctcgagcgg ctgtcggccc tgcaccgtcg
    16141 cgagctgcgc cgcttccgcg atgagatggg cgccctgctc gacgacctgg actagcgcga
    16201 ggtcggcgcg gtgaccgggt ggtcgagcgc gacggcctcg ccgaccagct gctcgctgtg
    16261 cacccgggcg acggcgtcgg ccagctcgcg tgcccggtcg gcgcgggggc cgacccaccc
    16321 gagctcgatc gtctcgtgga acagcgtgag ccaccgccgg aagtgggcgg gcgtgaacgg
    16381 ggcgcgagcg tggatctcgg catgggaccg gaacggattc ccggcgtagc ccggctcgcc
    16441 gatcagggcg cgcgcccaga acgcggtcag cttcgggagg tgctcggacc agtccacctc
    16501 ggcgacgtcc tcgaagacgg ggccgaggag gtcgtccatg gcgacgtcgg cgtagaagcg
    16561 ccggaccatc tcggcgatct cctccacgga gtcgaggtcc tcggtggggc ggggtcgggg
    16621 ggtgtctcgc acgggtttgc tcctgccggt ttcgatcgtg gtgggatata gttctagcat
    16681 ctactagaga aaccgaggag cgtcatgacc gagatcgtcc agaccatcga gcgcatcacc
    16741 gccgagagcg cggccctccg gggcgccctg atcggcacgg tgatcggctt cttcgcggtc
    16801 gggggcttca cgttctgggt cgccctcaac gccgggacct ccaccgtcgc cgcggtcggg
    16861 ctggcgctct tcgccgggtt ctggggcggc cccgggttcg gcggcatgct cggcgccacc
    16921 ttggccgtga cccgcaacga ggagcgcgag cgggaagccg ctgccgcatg accgacatcg
    16981 tgcaggagct ggtcgagcgc aagtacgagt tcggcttcca ctccgacatc gacaccgagc
    17041 tggccccacg tggcctgaac gaggacgtgg tccggctcat ctcggccaag aaggacgagc
    17101 ccgagtggct gctcgagtgg cgcctcgcgg cgtaccggca ctggacgacg atggagccgc
    17161 cggtgtggca gaacgtgcag cacccgccga tcgacttcca ggacatgcac tactgggcgg
    17221 cgccgaagcc gaagggaccg gcaccggcga gcctggacga cgtcgacccg gagatccggg
    17281 cgaccttcga caagctcggc atctcgctgg ccgagcagga gcgcctgtcc ggcgtcgccg
    17341 tcgacgccat catcgactcg gtctcggtgg ccaccacgtt caaggcgacg ctcgccgagg
    17401 cgggcgtcat cttctgctcc ttctcggagg cggtgcggga gcaccccgag ctggtgcgcc
    17461 gctacctggg atcggtggtg ccgccgaggg acaacttcta cgcggcgctg aactcggcgg
    17521 tgttctccga cggctcgttc tgcttcgtgc ctgccggcgt gcgctgcccg atggagctgt
    17581 cgacctactt ccggatgaac gcggccgaga ccgggcagtt cgagcgcacc ctgatcgtcg
    17641 ccgaggaggg tgcgacggtg agctacctcg agggctgcac cgcccccatg cgcgacgaga
    17701 accagctcca cgccgcggtg gtcgagatcg ccgcgatgga cgactccgac gtcaagtact
    17761 cgacggtcca gaactggtac ccgggcgacg agaacggcgt cggtggcatc tacaacttcg
    17821 tcaccaagcg gggtcgctgc gggcaccggg ccaagatctc ctggacccag gtcgagaccg
    17881 gatcagccat cacctggaag taccccagcg tgatcctcca gggcgacgac tcggtcggcg
    17941 agttctactc cgtggcggtc acgaccgggc gccagcaggc agacaccggc acgaagatga
    18001 tccacatcgg caagaacagt cgcagcacca tcgtctccaa gggcatctcg gccggcaagg
    18061 cccagcagac ctaccggggc ctggtgaagg tgctgcgctc cgccaccggt gcacggaacc
    18121 acacgcagtg cgactcgctg ctcatcgggt ccgagtgcgg cgcccacacg ttcccctaca
    18181 tcgaggtcaa gaacgactcg gcccaggtcg agcacgaagc ctcgacctcg aagatcagcg
    18241 acgaccagct cttctactgc cgccagcgag gcctcgacga ggaggacgcc acgtccatga
    18301 tcgtcaacgg cttctgccgg gaggtcttcg acgagctgcc catggagtac gcggtcgagg
    18361 cccacgcctt gatgcgcctg agc
//
LOCUS       DATISU010000045         5778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_654143, whole genome shotgun sequence.
ACCESSION   DATISU010000045 DATISU010000000
VERSION     DATISU010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654143"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VLR27_02085"
     rRNA            <1..630
                     /locus_tag="VLR27_02085"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            711..827
                     /gene="rrf"
                     /locus_tag="VLR27_02090"
     rRNA            711..827
                     /gene="rrf"
                     /locus_tag="VLR27_02090"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            925..2163
                     /locus_tag="VLR27_02095"
     CDS             925..2163
                     /locus_tag="VLR27_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HSP02261.1"
                     /translation="MPSRPEDDDRPGRRTGGGARRPSGQGRPRSDRARQAGDDRKPRP
                     GSRSRSDGASTGGASRGGGRGRDDRGRPTRSSDPRGGPRRSGADRDRGPRRDDRDGER
                     PERGPRPPIKGKRRTFDVDSAPNARERARPAEGEQPRGERWVDEGPVRKAAGEAVERG
                     SRRAGPPKGRSGGGGGGRGRDGAPKKSTGGRSSQRRAERATKRVEGALEIDRDRLRRR
                     LGQARADRTLGRIGEATAAYAEDRFEDVRKAMKPIAELIPEEPAVRELYGLSLYRLGK
                     WRQAIDELEEFARRSGSTEQHPVLADCYRALGQHDRVQELWDEIGEASLAAPLVAEGR
                     IVYAGSLADRGMLVEAIAVLEAGALGDKTLKDHHLRMRYVLGDLYDRAGEQQSARRWF
                     ESVASASPGFFDVQDRLRQL"
     gene            2267..2635
                     /locus_tag="VLR27_02100"
     CDS             2267..2635
                     /locus_tag="VLR27_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02262.1"
                     /translation="MPEPTTTDSDLDLAVLVGTLSSDPVEQTLPSGSVLLRYEVTVRD
                     RAPADTVPVAWVDPRRPPSLAVGDRVVVVGRVRRRFFRAGGATRSATEVLATSVSRPR
                     STSAARAALAEAAELVAGSR"
     gene            2762..3646
                     /locus_tag="VLR27_02105"
     CDS             2762..3646
                     /locus_tag="VLR27_02105"
                     /inference="COORDINATES: protein motif:HMM:NF003784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HSP02263.1"
                     /translation="MNQEQFDKVKSRQGFIAALDQSGGSSPKALRAYGVEEHEYDGDE
                     EMFDRIHQMRTRIMTSPAFTGDRVLGAILFEDTMDRTVEGKDSADYLWSVKQVVPFLK
                     VDKGLDDEVDGVQIMKPIPGLDDLLERAVSKGVFGTKMRSVIKEANANGVDAIVEQQF
                     AVGKEIVAAGLVPILEPEIDIHSATKAEAEALLRDGILARLDQLGDDIVMLKLTLPEE
                     DGFYQSLVDHPNVLAVVALSGGYSRDEANDRLSRNPGVIASFSRALTESLRAQQSAEE
                     FDAALDEAIESIYRASIA"
     gene            3757..5658
                     /locus_tag="VLR27_02110"
     CDS             3757..5658
                     /locus_tag="VLR27_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HSP02264.1"
                     /translation="MSDTSTQPTGERTESIDRVIIRFAGDSGDGMQLTGDRFTSASAM
                     FGNDLATLPDFPAEIRAPAGTIAGVSAFQVHISDHDITTPGDAPDVLVAMNPAALRSD
                     LHRLKGNGSVILNIDAFTERNLDKAGYTSDPRDDGSLDAYKVYEVPMTEMTKKAVEHL
                     GVKPRDAERSKNFFALGLVSWMYTRPAEHTLEWIQQKFGKDQQVADANVAAFKAGHAF
                     GETAELFDHAYSVKPAEKVPGTYTNITGNVALAWGLIAASQLADMPLFLGSYPITPAS
                     DILHELSKHKNFGVRTLQAEDEIAGVGSAIGAAYGGHLAITTTSGPGVALKGEAIGLA
                     VSLELPLVVVDIQRGGPSTGLPTKTEASDLLMAMYGRHGESPMPIVAAATPAGCFDAA
                     IEACRIALKYRTPVFLLSDGYLANGAEPWKLPDVESLPDISTTFATEANGTNDAGNPV
                     HHPYERDPETLARSWALPGTPGLEHRIGGLEKADKTGNVSYDPGNHELMTHLRQAKLD
                     GIAKDIPEAVLEGDADADLVVIGWGSTWGAISGGVDRVRAAGHSIARIHLTHLHPFPP
                     NLGDLLSRFDRALVPELNLGQLSKVLRAEFLIDAKTVSKVRGLPFTAVEIEAAILDSL
                     GVAQNGAAK"
BASE COUNT          946 a         2086 c         1936 g          810 t
ORIGIN      
        1 gtaacggagg cgcccaaagg ttccctcagg ctggttggca atcagccgtc gagtgcaatg
       61 acacaaggga gcttgactgc gagacctaca agtcgagcag gtacgaaagt agggcatagt
      121 gacccggcca ttgcttgtgg aagcgtggtc gctcaacgga taaaaggtac tccggggata
      181 acaggctaat ctttcccaag agtccatatc gacggaaagg tttggcacct cgatgtcggc
      241 tcatcgcatc ctggggctga agcaggtccc aagggttggg ctgttcgccc attaaagcgg
      301 tacgcgagct gggtttagaa cgtcgtgaga cagttcggtc cctatccgct gcagccggaa
      361 gaagcttgag gaaggctatc cctagtacga gaggaccggg atggacgcag ctctcgtgtg
      421 ccagttgtcc tgccaagggc acggctggtt agcgacctgc ggaaaggata agcgctgaag
      481 gcatctaagc gcgaaacctg ttccaagatg aggcttctca ctgggtcaac caggtaaggc
      541 ccgtggtaga ccaccacgtt gataggccgg atgtgtacgc acagcaatgt gttcagcaga
      601 ccggtactaa tcggccgagg gcttggtttc atccatgttc gtgctcgcta tggaatgctc
      661 gaggaacact cgaaggccgt ctcggcctcg tgttcgttga caaccaaatt ctcggtggtg
      721 atagcaacgg agatacaccc gttcccattc cgaacacgga agttaagccc gtttgcgccg
      781 atggtacttg gggggagacc ccctgggaga gtaggtcacc gccggtttct tgaagggccc
      841 ccgcattgcg ggggcccttc gccgttttcg gcctcggttt ccggtcttcg tggcccggac
      901 cggggcgcct gtaccatcgt cgggatgccc tcgcgccccg aagacgacga tcgacccggc
      961 cgacggaccg gtggcggcgc ccgccgcccc tccggccagg gccgcccccg ctcggatcgg
     1021 gcccgccagg ctggcgacga tcgcaagccc cgtccgggga gccggtcccg cagcgacggc
     1081 gcctccacgg gcggtgcctc gcggggcggt ggccgagggc gcgacgatcg cggccgcccg
     1141 acccgcagca gcgacccgcg tggcggtccc cgtcgctcgg gtgccgatcg cgatcgcggg
     1201 ccgcgccgcg acgaccgcga cggcgagcgg cccgagcgtg gtcctcgtcc gccgatcaag
     1261 ggcaagcgcc gcaccttcga cgtcgactcc gcgcccaacg cccgcgagcg ggcgcgcccc
     1321 gccgagggcg agcagcctcg cggtgagcgc tgggtcgacg aaggaccggt gcgcaaggcc
     1381 gccggcgagg ccgtggaacg cggcagccgg cgcgccggcc cgccgaaggg ccgaagcggc
     1441 ggtggtggtg gtggtcgcgg ccgcgacggg gcgcccaaga agtccaccgg cggtcggagc
     1501 tcgcagcgtc gggccgagcg ggccaccaag cgcgtcgagg gtgcgctcga gatcgaccgc
     1561 gatcggctcc gccggcgcct gggccaggcc cgggccgacc ggacgctcgg tcggatcggc
     1621 gaggccaccg cggcgtacgc cgaggatcgc ttcgaggacg tccgcaaggc gatgaagccc
     1681 atcgccgagc tgatccccga ggagccggcg gtgcgcgagc tgtacgggct gtccctgtac
     1741 cggctcggga agtggcgcca ggccatcgac gagctcgagg agttcgcccg gcggtctggt
     1801 tccaccgagc agcacccggt cctggccgac tgctaccggg ccctcggtca gcacgaccgg
     1861 gtccaggagc tgtgggacga gatcggggag gcctccctcg ctgcgccgct cgtggctgaa
     1921 gggcgcatcg tctacgccgg ttcgctggcc gaccggggca tgctcgtcga ggccatcgcc
     1981 gtgctcgagg ccggcgcgct cggcgacaag acgctcaagg accaccacct gcggatgcgc
     2041 tacgtgctcg gtgacctcta cgaccgcgcc ggcgagcagc agtcggcgcg ccgctggttc
     2101 gagtcggtgg cgtccgcgtc ccccggcttc ttcgacgtcc aggaccgcct gcgccagctc
     2161 tgagcccgcg ggttcctgtc agaccccccg ggagacactg ggaccggctt cccccagcac
     2221 ctgcccgccg gccgcgccgg cggtgctcct gcatgcggag gctcccgtgc ccgaacccac
     2281 caccaccgac tccgacctgg acctcgccgt cctcgtcggc accctcagca gcgacccggt
     2341 cgagcagacc ctgccctcgg gctcggtgct gctccgctac gaggtcacgg tgcgcgaccg
     2401 cgcaccggcc gacaccgtcc ccgtcgcctg ggtcgatccc cgacgcccgc catcgctcgc
     2461 cgtgggcgac cgcgtcgtgg tcgtcggtcg cgtccgccgg cggttcttcc gagccggcgg
     2521 cgccacccgc agcgccacgg aggtcctggc cacgtcggtg agccggcccc ggagcacctc
     2581 ggcggcccgt gccgccctgg ccgaggccgc cgagctcgtc gccggttctc gctgagcctc
     2641 cggcggggga gaaggtgaac ggtcgcgtgg ccgtcggcca ggatgggcac cgcagcggca
     2701 cccgtcgcgt cgtcgccgcc cgaccacccc cacccaccgt ccccacaggg agagcaagcg
     2761 gatgaaccag gagcagttcg acaaggtcaa gagccggcag ggcttcatcg ccgcgctcga
     2821 ccagagtggt ggcagcagcc ccaaggcgct gcgtgcctac ggcgtcgagg agcacgagta
     2881 cgacggcgac gaggagatgt tcgaccgcat ccaccagatg cgcacccgga tcatgacctc
     2941 gccggcgttc accggcgatc gggtgctcgg cgccatcctg ttcgaggaca ccatggaccg
     3001 cacggtcgag ggcaaggact cggccgacta cctgtggtcg gtgaagcagg tcgtgccgtt
     3061 cctcaaggtg gacaagggcc tcgacgacga ggtcgacggc gtgcagatca tgaagccgat
     3121 ccccggcctc gacgacctgc tcgagcgggc cgtttccaag ggcgtgttcg gcaccaagat
     3181 gcgctccgtc atcaaggagg ccaacgccaa cggcgtcgac gccatcgtcg agcagcagtt
     3241 cgcggtcggc aaggagatcg tcgccgccgg cctggtgccg atcctcgagc cggagatcga
     3301 catccacagc gccaccaagg ccgaggccga ggcgctcctg cgcgacggca tcctcgctcg
     3361 cctcgaccag ctgggcgacg acatcgtcat gctcaagctg acgctgcccg aggaggacgg
     3421 cttctaccag tccctcgtcg accacccgaa cgtgctcgcg gtggtcgccc tgtccggtgg
     3481 ctacagccgc gacgaggcca acgaccgcct ctcccgcaac cccggcgtca tcgccagctt
     3541 ctcgcgagcc ctcaccgaga gcctccgcgc ccagcagtcg gcggaggagt tcgacgccgc
     3601 gctcgacgag gcgatcgaga gcatctaccg ggcatccatc gcctgacgag ggacctctga
     3661 ccctgccaga ccgggactcc ggcctcgcgc gcatccgtcg cgcgcagggc tagggtcgac
     3721 cccgttgaca acgacgtcat cggagtaggg ggacaggtgt cagacacctc cacacagccc
     3781 accggagagc gcacggagtc catcgatcgg gtcatcatcc gcttcgcggg tgactcgggc
     3841 gacggcatgc agctcacggg ggatcggttc accagcgcgt cggccatgtt cggcaacgac
     3901 ctggccacgc tgccggactt cccggccgag atccgcgcgc ccgccggcac gatcgcgggc
     3961 gtctcggcgt tccaggtcca catctcggac cacgacatca ccacgccggg cgacgcgccc
     4021 gacgtgctcg tggccatgaa cccggccgcg ctgcgcagcg acctgcaccg gctcaagggc
     4081 aacggctcgg tgatcctcaa catcgacgcg ttcaccgagc ggaacctgga caaggccggc
     4141 tacacgtccg acccccgtga cgacgggagc ctcgacgcct acaaggtgta cgaggtgccg
     4201 atgacggaga tgacgaagaa ggcggtcgag cacctgggcg tgaagccccg cgacgccgag
     4261 cgatccaaga acttcttcgc cctcggcctg gtgtcgtgga tgtacacccg tcccgccgag
     4321 cacaccctcg agtggatcca gcagaagttc ggcaaggacc agcaggtcgc cgacgccaac
     4381 gtcgccgcgt tcaaggccgg gcacgccttc ggtgagaccg ccgagctgtt cgaccacgcc
     4441 tactcggtca agccggccga gaaggtgccg ggcacctaca ccaacatcac cggcaacgtc
     4501 gccctggcct gggggctcat cgccgcgtcc cagctggccg acatgccgct gttcctcggc
     4561 tcgtacccga tcaccccggc ctcggacatc ctccacgagc tgtccaagca caagaacttc
     4621 ggcgtgcgca ccctgcaggc cgaggacgag atcgccggcg tgggctcggc catcggtgcc
     4681 gcctacggcg gccatctggc catcaccacc acgtccgggc ccggcgtggc cctgaagggc
     4741 gaggccatcg gcttggccgt gagcctcgag ctgcccctgg tcgtcgtcga catccagcgc
     4801 ggtggcccgt ccaccgggct gcccaccaag accgaggcgt cggacctgct gatggcgatg
     4861 tacggccgcc atggcgagtc cccgatgccg atcgtggccg ccgcgacgcc cgcgggctgc
     4921 ttcgacgccg ccatcgaggc gtgccggatc gccttgaagt accgcacgcc ggtcttcttg
     4981 ctctccgacg gctacctcgc caacggcgcc gagccgtgga agctgcccga cgtggagtcg
     5041 ctgcccgaca tctccaccac cttcgccacc gaggccaacg gcaccaacga cgccgggaac
     5101 ccggtgcacc acccctacga gcgcgatccc gagacgctgg cccggtcgtg ggcgctgccg
     5161 ggcacccccg gactcgagca ccgcatcggc ggcctcgaga aggccgacaa gaccggcaac
     5221 gtctcctacg acccgggcaa ccacgagctc atgacgcacc tgcgccaggc caagctcgac
     5281 ggcatcgcca aggacatccc cgaggccgtg ctcgaaggcg acgccgacgc cgacctggtc
     5341 gtgatcggct ggggctccac ctggggtgcg atctccggcg gcgtcgaccg cgtgcgcgcc
     5401 gccggccact cgatcgcccg gatccacctc acgcacctgc acccgttccc gccgaacctg
     5461 ggcgacctgc tgagccggtt cgaccgggca ctggtccccg agctcaacct cggccagctc
     5521 agcaaggtcc tgcgggccga gttcctgatc gacgccaaga ccgtcagcaa ggtccgcggc
     5581 ctgccgttca cggccgtcga gatcgaagcc gccatcctcg actccctcgg ggtcgcgcag
     5641 aacggagcag cgaagtgagc gacaccacca tcccagagac cagccgcaag gactggatgt
     5701 cggaccagga ggtccgctgg tgcccgggct gcggtgacta cagcatcctc gccgccgtgc
     5761 agctggcgct gcccgaca
//
LOCUS       DATISU010000046        16855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_607628, whole genome shotgun sequence.
ACCESSION   DATISU010000046 DATISU010000000
VERSION     DATISU010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 16855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16855
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607628"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VLR27_02115"
     CDS             <1..762
                     /locus_tag="VLR27_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HSP02265.1"
                     /translation="YHQTRRLAADFAERGWMVVTGAGPGIMAAGMEGAGRENSIGVTI
                     RLPVEESANEFIDGDDKLVAMKYFFTRKLMLVKESKGFVALPGGFGTQDETFELFTLQ
                     QTGKSVPAPVVLLDVPGGTYWSSWVTFVKDELVAGGLVSPGDLELFTVTDDVDVACEE
                     IVGFYRNYDSIRWAGDRLIIRMRQGPDDAQLEALNEAHAHLLTEGRIERTEPTPAERS
                     SNDALGLDRVVMHYDKWRQSSIHRIIRDVNAWTPA"
     gene            747..1898
                     /locus_tag="VLR27_02120"
     CDS             747..1898
                     /locus_tag="VLR27_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP02266.1"
                     /translation="MDARVSGLALTDDDLDASRDRLRAWLAANPDPDGATLAAAGLVA
                     PHYPPPWGVAAGPELQLLIDAELAAAGVTGPENPIGIGWAGPTLVAAGTPEQQQRWLP
                     GLLSGADFWSQLFSEPEAGSDLASLRTRAERDGDVYVVNGQKIWSTWADRAAYGILLA
                     RTDPAAPQHRGISYFVLDLSLPGIDVRPITEMTGQSHFNEVFLTDVRVPTDCRIGDEG
                     DGWRLAKVTLGNERVSLSEGGVCWGMGPTSSDAFELLRREGVTGAVARQEAARLWSQA
                     KVLELLDQRVVEAVVRGEDPTPVASLKKRLADVHGQELLTFVKDRRGPHGMLGEQGPH
                     AEEADVWHWAYLFSRALTIGGGTTEVLSNIIAEQLLGLPREPRPRGGTT"
     gene            1895..2686
                     /locus_tag="VLR27_02125"
     CDS             1895..2686
                     /locus_tag="VLR27_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSP02267.1"
                     /translation="MSHEFTTIDFERDGDVLVVTIDNPTSPVNAVDGPLHEDLAALFP
                     RLQAERDARAVLLTGRGKAFSAGGDFDWFPTLQDATALEHLRLDAKAMIWDLLDVHLP
                     IVAAVNGHAMGLGASVALLCDVVFVSQRATIGDPHVKVGLVAGDGGTIAWPLALGPML
                     AKRFLLTGDAVGADEAVRLGLVHRAVPPEELQAEALAFAHRLADGAPLAVQYTKAAIN
                     AWIKSTAAPSFDLAQAYEIATFRSEDHREALDALAEDRPPGFRGR"
     gene            2880..3638
                     /locus_tag="VLR27_02130"
     CDS             2880..3638
                     /locus_tag="VLR27_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02268.1"
                     /translation="MPADDHPPSQPETYEDLHELVTFEDPDEYRTWVFDVTFLTSSWT
                     CIFGQGCPGVLTEAAPELEQGCCSYGAHFVDDEDHDETQARIDSLTPEQWQFHKQGNR
                     DGATRKDKSGQWVTRLHKGACILLNRPGHPNGAGCALHQAAEQAGERPLDWKPDVCWQ
                     LPLRLEESTDDHGHVTSTLREWKRRDWGGGGAEFHWWCTDDPLAFRAAQPTYEVLRDE
                     IIELVGETPYRLFVELIERRREWTPLPHPALRRR"
     gene            complement(3656..4699)
                     /locus_tag="VLR27_02135"
     CDS             complement(3656..4699)
                     /locus_tag="VLR27_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02269.1"
                     /translation="MFRRVLGVCLLFVAACGSGETTPVERAGVDPQQAPDVTLPAPLT
                     TEAPASTSTVPPADGEVTEQAATPALPAPIEPERAEPWTLEPYRGFGAWLDAYDWSVT
                     FARSPDHVIGLDQIDHMAREGVETIYIQASRWNSPEDILEPERLQGIIDRAHDNGMSV
                     VAWYLPTFVDPETDLRRMIAIASMDFDGFGVDIESRDVADVAERNRRLMDISARLESA
                     LEGRPLAAIVMEPVIMEDVNPQFWPDYPWMGLAQHYDVWLPMSYWTNRRGAWRNAHTY
                     TAENIARIRQRIGQPDAPVHTIGGIGDKTTVDDLHGMVLAAAEHGAIGGSIYDYRTTR
                     GEFWDVLRAFRSE"
     gene            complement(5036..6193)
                     /locus_tag="VLR27_02140"
     CDS             complement(5036..6193)
                     /locus_tag="VLR27_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HSP02270.1"
                     /translation="MAEPATARWIDTGEGLAELVAELADAPRLAIDTEFHRERTYWPQ
                     CALVQIGWGDGEALVDPLAVDITPLGALLTGRTIVMHAAGQDLEVFQHSVGVVPDDLF
                     DTQIAASFLGMSSIGLAPLVNKMLGIHLPKADRLTDWLARPLSEDAKRYAQSDVVHLF
                     ELHDQMTASLEQRGRMGWVRSECEAMVERSLAPSDPAMAWWRVKEARRLKGTAAGVAQ
                     SLAAWREAHAAEVDRPLRSVLPDLALAGIAQRPPKTVEDLVKIRGLRDRGIPKSIRDD
                     VLDAVAAGKALDPEDVVYPEDRPVPAELRGAVPLLMSWVSQRARQLELDPAVLATRGD
                     LEGFLRDDPDNRLATGWRAEALATDIGDLLEGRAGLSFAKGEGLVLRPLDP"
     gene            complement(6186..6656)
                     /locus_tag="VLR27_02145"
     CDS             complement(6186..6656)
                     /locus_tag="VLR27_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSP02271.1"
                     /translation="MFAAGERSLVGAALSLRIATLDDVAPAAVHDADRPEDLPSPPSG
                     AGTPSIGTVQVPEIDITALRQKLDDGAPLFDVRQPDEYEQGHAPGARLVPLGEVPDRV
                     GEFPTDETVYVICRSGGRSGKAVEHLRANGVDAVNVAGGTLAWVEAGNAVEQGG"
     gene            complement(6639..7103)
                     /locus_tag="VLR27_02150"
                     /pseudo
     CDS             complement(6639..7103)
                     /locus_tag="VLR27_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
     gene            complement(7106..8029)
                     /locus_tag="VLR27_02155"
     CDS             complement(7106..8029)
                     /locus_tag="VLR27_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02272.1"
                     /translation="MPAPVRRSRLVLLLLTGLLVAAACGTDAEEQLAVTSTTTTAPQA
                     STPGQPAPADEQPASDVEGTPQGTAPPAASAPDASGAPAPGETTPGGDAEPVPAAGPV
                     GSLAGWYLRPAGAEQILVQVLAQDGAGPRTQAPERVRQVLAQLSGKPVAVSVGAVGGG
                     ARSWTADELRAIADGSSPAQTAEQGVLTVLFLHGGFAENDRAVGVAVRGDVAAVFAER
                     VDEASGMLGNPGAVEDAVTMHEVGHLLGLVDLVLDTGRQDPEHPGHSPNRGSVMYYAV
                     ESTVLGSVLSGGPPRDFDQADLDDLARIRNG"
     gene            complement(8036..8467)
                     /locus_tag="VLR27_02160"
     CDS             complement(8036..8467)
                     /locus_tag="VLR27_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 family protein"
                     /protein_id="HSP02273.1"
                     /translation="MSQSVNLSASGPPETVLDPEPADALERLAAASDLPEDDRRRAVA
                     AVVADHPRCLHAWAALGDGARDRIEAYAAYRVGYHRGLDRLRANGWRGSGYVRWTHPE
                     NTGFLRSLDGLRRAAEAIGETDEAERCAQFLAQLDPAGRPS"
     gene            8532..8804
                     /locus_tag="VLR27_02165"
     CDS             8532..8804
                     /locus_tag="VLR27_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02274.1"
                     /translation="MGTILAAAPYEEFVRDNSPVLAVVACLILALLVIRLVVKTMTRM
                     ILLSTLLLMVLFVYVERDNIQECTQTCECAIAGIDVELPACDPARI"
     gene            complement(8801..9601)
                     /locus_tag="VLR27_02170"
     CDS             complement(8801..9601)
                     /locus_tag="VLR27_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP02275.1"
                     /translation="MTGEDFVADGRCWRDAAGEGPGVVLVHGTMDRSSSFGRVRKALS
                     DMHVARYDRRGYARSVALGPPEAFHQQVDDLLDVIGWLVGDGPPPVVFGHSYGGTVAL
                     AAAAEAPERLAGVVAFEAPLPWMDWWPSGSAGAAAVASADDPEDAGEKFMRRMVGDQR
                     WERLPRRTRDARRAEGPTLVAELAQLRPPHPPPFELTSIRVPVLAAHGTEGAAHHGEA
                     TRAVAETVPGADLEVVQGSGHGVHLTHPAAVAAMVRRLVADLDLVPEG"
     gene            complement(9598..11022)
                     /gene="pyk"
                     /locus_tag="VLR27_02175"
     CDS             complement(9598..11022)
                     /gene="pyk"
                     /locus_tag="VLR27_02175"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HSP02276.1"
                     /translation="MTRRTKIVATIGPASDSEEVLRAMMLAGVDVCRLGLAHGTIEEH
                     QLRIKRIRKVASEMNRPISVLGDLPGPKIRMGPVIEEGGLFLAEGQRIEIRPGDAPTA
                     PDHIHVDYPHLLEAVAVGDSLVLGDGSVRLVAVENDGSRLMVEVEHGGRIQGRPGVTL
                     PSAKLRVSVPTDQDIALLEGLAGELDFVAASFVRSADEVAAVKALAQGADVVAKIETK
                     AAVDALDAIVNIADAVMVARGDLGNECDIAEVPHLQKQIIRQGVSFGVPVITATQMLE
                     SMIHSPSPTRAEATDVANAVFDGTTAVMLSAETAIGHNPTLVIKTMSHLCERAEAAAD
                     YSTWGSQLGRIQGQVQLDLTTRITAGMSHAAWRLATELELDGIISCTNSGYTARAISR
                     FRPPGVLIGASPFEDTVRKMSLVWGTKPLKMDEYSTTDEMVWHAVEKSVKAGYIKAGN
                     IVAVLAGTADDIGTTDVLRVVRVT"
     gene            11054..11467
                     /gene="arfB"
                     /locus_tag="VLR27_02180"
     CDS             11054..11467
                     /gene="arfB"
                     /locus_tag="VLR27_02180"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HSP02277.1"
                     /translation="MAGDDDLVTSRGLRVPSSAIRWWASRSGGPGGQHANTSDTQVTV
                     ELDLDAADLPEGVTARLRTAHGDAVRASSSDSRSQYRNRQLARKRLADLVDGAAKPPA
                     RRRPTKPSRGAKRRRLEAKRQQSEKKARRSWRPER"
     gene            complement(11524..12738)
                     /locus_tag="VLR27_02185"
     CDS             complement(11524..12738)
                     /locus_tag="VLR27_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP02278.1"
                     /translation="MAVTERTPTVTDPDEQRVLDAIDSLLEAHPPKDTDVVDFLGAQF
                     DAGLAWVHYDEGFGGLGLPARFQKVINERLAAAGAPNAAGRNPIGHGMSAPTIYTHGS
                     DEQKGRYLRPLFTGEEIWCQLFSEPGAGSDVAGLSSRAVKDGDEWIINGQKVWTTLAH
                     VSRFGTLVARHDPEAGKHAGLTHFVVDMQAPGVEVRPLRQMTGDAEFNEVYFTDVRVP
                     DAERLGEVGEGWRVAITTLMNERVSIGGAIPPRESGPIAIAKKAWQEHCDTEDGAVKD
                     ELMQLWVRAEVSRLTNIRASQMRKLGDPGPEGSVAKLAMAELNKDIMSFAVDCMGAHG
                     MLYGSYDMERTGGVGMFGGSTQQSFLRSRANSIEGGTTEVMKNILGERVLGLPGDIRV
                     DKDKPWSEVPRS"
     gene            complement(12769..13341)
                     /locus_tag="VLR27_02190"
     CDS             complement(12769..13341)
                     /locus_tag="VLR27_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HSP02279.1"
                     /translation="MIDRDVKQCLGQMTKGVQVVAARHDGVTRAYTSHWISQISFEEP
                     VLLASVSPKHDTHPLIEAAGWFSVSILAGDQVEEGQYFSYPGRKFHRLFDEYLTETEV
                     DGFGPVPHVVDCLAWLRCEIREKLDLGLDHDCYVVDVRDVGPGRLKADPLVYSSRHGW
                     RVASEKARTPGVSVRDRLLERLEEAGLDES"
     gene            complement(13369..14286)
                     /locus_tag="VLR27_02195"
     CDS             complement(13369..14286)
                     /locus_tag="VLR27_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HSP02280.1"
                     /translation="MTSSGPPRCWSPWSRRATRRIPTTSWPRPAGCGPASTENRWVAG
                     APGRTVRATSSTAIPAWLVDDPVVEHDLGTLRSGKEAQIDVIERVGADGRTCLLARKR
                     YLPRTVTSKGQLEALGVQRSSAFRHDVEYREGRQFRKSRDRRAVETMTTYGRKLLQDR
                     WLDHEHDVLGRLHDAGCRVPYPVSYADDVLLMQYVGDLEQAAPQLAAARLTPDEVRAA
                     WHDVVDGLRRITAAGWAHGDLSAYNLLWWDGEVWFIDLPQAVDIAANPGGLSFLHRDV
                     CNVAAWFARRGIEDDPEEVFADLLSAAFG"
     gene            complement(14174..14941)
                     /locus_tag="VLR27_02200"
     CDS             complement(14174..14941)
                     /locus_tag="VLR27_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HSP02281.1"
                     /translation="MTERFADLVSQPDFPLDEAALLIAVHADPGCDVAANLARLDELA
                     RSVPEPTLPALTTSLFGRWGFVGDQVDYYDPRNSYLHEVLERRRGIPITLSVALIEVG
                     RRAGVPLAGVGTPAHFMTCTTTGTRRWVDAFAGGRILDRDELDDQFSRLAPGIDLDAY
                     LDPVPPRAIVARMLGNLVAIHRQRNDRTALLWASRLRTLVPGSTPDDRRAYGGALAAC
                     GDFVRAAKVLESLVEEGHTSDPDDELAKARRLRARLN"
     gene            15090..16646
                     /locus_tag="VLR27_02205"
     CDS             15090..16646
                     /locus_tag="VLR27_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP02282.1"
                     /translation="MYPGAFAATRPDHPAVIMAGTGQTMTYAELDEEANRLSQLLRSV
                     GLQPGDHVAFCMENHPRFLAVAWGCHYAGLYYTAMSSRLNDDEMQYILNDCGAKAFIT
                     TAYKREAAEKLLGQAPDVETWLMIDGTVDGYESYEDTIAAQPAEPLEGRVAGADMLYS
                     SGTTGKPKGVKPPMPSQPLEEAGTSVYGLMAMLFGLTEDSMYLSPAPLYHAAPLRFCM
                     GVHMSGATVVVMERFDPEEALELIEQHRITLSQWVPTMFIRMLKLPEDVRAKYDVSSL
                     QVAVHAAAPCPVEVKRRMIEWWGPVLHEYYAGTEGNGFVYCNSEDWLEHPGSVGKALI
                     GTIHIVGEDGEELPVGEPGTVYFEGENAATFQYHNDPEKTEDSRDPKGRGWSTLGDVG
                     RLDEDGFLYLTDRKAYMIISGGVNIYPQEAENVLAMHPKVYDVAVFGVPNEDFGEEVK
                     AVVQPAEGIEPTPELERELIAFCREKLADVKCPRSIDFRDELPRHPTGKLYTRLLKDE
                     YWQGHESGII"
     gene            complement(16639..>16855)
                     /locus_tag="VLR27_02210"
     CDS             complement(16639..>16855)
                     /locus_tag="VLR27_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02283.1"
                     /translation="VVRLNPTAPAAATGITQTGVYVGALTGPILFGGVFDSAGYGLGW
                     LLAGVASALAAAGVVYGRSRVVASRLR"
BASE COUNT         2370 a         6107 c         6091 g         2287 t
ORIGIN      
        1 taccaccaga cccgccgcct ggccgccgac ttcgccgagc ggggctggat ggtggtcacc
       61 ggcgccggcc ccgggatcat ggccgccggc atggagggcg ccggccggga gaactccatc
      121 ggcgtcacca tccggctgcc cgtcgaggag tcggccaacg agttcatcga cggcgacgac
      181 aagctcgtgg ccatgaagta cttcttcacc cgcaagctca tgctggtgaa ggagtccaag
      241 ggcttcgtgg cccttcccgg cgggttcggc acccaggacg agaccttcga gctgttcacc
      301 ctgcagcaga ccggcaagag cgtccccgcc ccggtcgtgc tcctcgacgt cccgggcggc
      361 acgtactggt cgtcgtgggt gaccttcgtc aaggacgagc tggtggccgg ggggctggtg
      421 agcccgggcg acctcgagct gttcaccgtc accgacgacg tcgacgtcgc ctgcgaggag
      481 atcgtcggct tctaccgcaa ctacgactcc atccgctggg ccggcgaccg cctcatcatc
      541 cggatgcgcc aggggcccga cgacgcccag ctcgaggccc tcaacgaggc gcacgcccac
      601 ctgctcaccg aggggcgcat cgagcggacc gagccgaccc ccgccgagcg ctccagcaac
      661 gacgccctcg gcctcgaccg cgtcgtgatg cactacgaca agtggcgcca gagctcgatc
      721 caccgcatca tccgcgacgt gaacgcgtgg acgcccgcgt gagcgggctc gccctcaccg
      781 acgacgacct cgacgccagt cgcgaccgac tgcgggcgtg gctcgccgcc aaccccgatc
      841 ccgacggcgc gacgctcgcg gccgccggtc tggtcgcccc gcactacccg ccaccctggg
      901 gcgtggccgc cggcccggag ctgcagctgc tgatcgacgc cgagctcgct gcggccggcg
      961 tcaccgggcc cgagaacccc atcggcatcg gctgggccgg ccccacgctg gtcgccgcgg
     1021 gcacgccaga gcagcagcag cggtggctgc cgggcctgct gtcgggtgcg gacttctggt
     1081 cccagctgtt cagcgagccc gaggccggtt ccgacctggc atcgcttcgc acccgagccg
     1141 agcgcgacgg cgacgtctac gtcgtgaacg gccagaagat ctggtccacg tgggcggacc
     1201 gggctgccta cgggatcctc ctcgcccgca cggaccccgc cgcgccccag caccgcggca
     1261 tcagctactt cgtgctcgac ctctccctgc ccggcatcga cgtccggccg atcaccgaga
     1321 tgaccgggca gtcgcacttc aacgaggtgt tcctcaccga cgtgcgcgtg ccgaccgact
     1381 gccgcatcgg cgacgagggc gacggctggc gcctggccaa ggtcaccctc ggaaacgagc
     1441 gggtgtcgct gtccgagggt ggcgtgtgct ggggcatggg cccgaccagc agcgatgcct
     1501 tcgagctgct gcgccgggag ggcgtcaccg gcgccgtggc ccgccaggag gccgcccggc
     1561 tgtggagcca ggccaaggtg ctcgagctgc tcgaccagcg cgtggtcgag gccgtggtgc
     1621 gcggcgagga cccgacgccg gtcgcgtcgc tcaagaagcg cctggccgac gtgcacggcc
     1681 aggagctgct gacgttcgtg aaggaccgcc gcggtccgca cgggatgctc ggcgagcagg
     1741 gcccccacgc ggaggaggcc gacgtctggc actgggccta cctgttctcc cgcgccctca
     1801 ccatcggcgg cggcacgacc gaggtgctgt cgaacatcat cgccgagcag ctgctcggcc
     1861 tgcccaggga gccccggccc cgaggaggca cgacgtgagc cacgagttca ccaccatcga
     1921 cttcgagcgc gacggcgacg tcctcgtcgt caccatcgac aacccgacct cgcccgtcaa
     1981 cgcggtggac ggcccgctgc acgaggatct cgctgccctc ttcccccgcc tgcaggccga
     2041 gcgggacgcc cgcgccgtcc tgctcaccgg gcgcgggaag gccttctccg ccggcggcga
     2101 cttcgactgg ttccccaccc tgcaggacgc caccgcgctc gagcacctgc gcctcgacgc
     2161 caaggccatg atctgggacc tgctcgacgt ccacctgccc atcgtggccg cggtgaacgg
     2221 ccacgccatg ggtctcggcg cgtccgtggc cctgctctgc gacgtggtgt tcgtgtccca
     2281 gcgggccacc atcggcgacc cccacgtcaa ggtcggcctg gtcgccggcg acgggggcac
     2341 catcgcctgg cccctcgcgc tcggcccgat gctggccaag cgcttcctcc tcaccggcga
     2401 cgcggtcggc gccgacgaag ccgtgcgcct cgggctggtc caccgcgccg tcccgcccga
     2461 ggagctccaa gcggaggccc tggccttcgc ccaccgactc gccgacgggg cgccgttggc
     2521 cgtgcagtac accaaggccg ccatcaacgc ctggatcaag tcgaccgctg ccccctcgtt
     2581 cgacctggcc caggcctacg agatcgccac gttccgctcc gaggaccacc gggaggcact
     2641 cgacgccctg gccgaggacc gccccccggg gttccgcggc cgttgatccg acggtcaccg
     2701 gccttcgacc gcctcacccg gatggcggca tccgactccg agcggcagtg gttcaagggc
     2761 gcctatggcc tgcccgcccc cgtcgggggc acgcgcgaga ccgccctgtc cacgccagcg
     2821 cctggtcctg accgccgggg acggcaccac ggcggggctg gggtagcgtc gtgcatccca
     2881 tgccggcgga cgaccacccc ccatcgcagc ccgagaccta cgaggacctc cacgagctgg
     2941 tgaccttcga ggaccccgac gagtaccgca cctgggtgtt cgacgtcacc ttcctcacca
     3001 gctcctggac ctgcatcttc ggccagggct gccccggcgt cctcaccgag gcggcacccg
     3061 agctcgagca gggctgctgc agctacgggg cccacttcgt ggacgacgag gaccacgacg
     3121 agacccaggc gcgcatcgac tcgctcaccc ccgagcagtg gcagttccac aagcagggca
     3181 accgggatgg cgccacccgc aaggacaaga gcggccagtg ggtcacccgg ctgcacaagg
     3241 gcgcatgcat cctgctgaac cggcccgggc accccaacgg cgccggctgc gcgctgcacc
     3301 aggccgccga gcaggccggc gaacgaccgc tcgactggaa gcccgacgtg tgctggcagc
     3361 tgcccctgcg cctcgaggag tccacggacg accacggcca cgtcacctcc acgctgcggg
     3421 agtggaagcg ccgcgactgg ggcggcggcg gggccgagtt ccactggtgg tgcaccgacg
     3481 accccctggc gttccgcgcc gcccagccga cctacgaggt gctccgagac gagatcatcg
     3541 agctggtcgg cgagacgccg taccggctct tcgtggagct gatcgagcgg cgccgcgagt
     3601 ggacgccgct gccccacccc gcgctgcgcc gccggtaggc ggctcggacc gggggctact
     3661 cggaccggaa ggcccgcagc acatcccaga actcgccccg ggtcgtgcgg tagtcgtaga
     3721 tgctgccccc gatggcgccg tgctcggcgg cggcgaggac catgccgtgg aggtcgtcga
     3781 cggtcgtctt gtcgccgatg ccgccgatgg tgtgcaccgg cgcgtcgggc tggccgatgc
     3841 gctggcggat ccgggcgatg ttctcggccg tgtacgtgtg ggcgttgcgc caggcacccc
     3901 gccggttcgt ccagtagctc atcggcagcc acacgtcgta gtgctgggcc aggcccatcc
     3961 acgggtagtc cggccagaac tgcgggttga cgtcctccat gatgaccggc tccatcacga
     4021 tggccgccag cggccggccc tcgagggcgc tctcgaggcg ggcggagatg tccatcagcc
     4081 ggcggttgcg ctcggcgacg tcggcgacgt cgcgtgactc gatgtccacg ccgaacccgt
     4141 cgaagtccat cgaggcgatg gcgatcatgc gccgcaggtc ggtctcgggg tcgacgaagg
     4201 tcggcaggta ccaggccacc accgacatcc cgttgtcgtg ggcccggtcg atgatgccct
     4261 gcagccgctc cggctcgagg atgtcctcgg ggctgttcca ccggctggcc tggatgtaga
     4321 tcgtctccac accctccctg gccatgtggt cgatctggtc gaggccgatg acgtggtccg
     4381 gcgatcgggc gaaggtcacc gaccagtcgt aggcgtcgag ccaggcaccg aatccccggt
     4441 agggctcgag ggtccagggt tcggcccgct cgggctcgat cggggcgggc agcgccgggg
     4501 tcgcggcctg ctcggtgacc tcgccgtcgg cgggcgggac cgtcgaggtg gaggcggggg
     4561 cctcggtggt cagcggcgcc ggcagcgtca cgtcgggtgc ctgttgggga tcgacgccgg
     4621 cgcgctcgac cggcgtggtc tcgcccgacc cgcaggccgc gacgaagaga aggcagaccc
     4681 ccagcacgcg tcggaacacg gtggaaaccc tacgtccccc tcgaccgccg cccaacgcgc
     4741 gggcggcgct cagctatgcg tcgtcggcgt ccgccaggca ccacgaggcg tgctcggccc
     4801 cgggcggggc atcgcactcc tcgcacggct ccggcggggc ttccccgaag atgtccgaga
     4861 tgtcaatgcc ggcgtccgag cgcttcagcg cgcgggcttc ttcgaaacgg cgatgtcgac
     4921 cctttttcac gggcgtgctc ccgactcgtg ggggctgaga atcgatcagt gtagcggcgt
     4981 gcagctcggc tccacgcagt gctccgagcc gattgtgaca cgccaccgga gcggatcacg
     5041 gatcgagcgg tcgcaggacc aggccctcac ccttcgcgaa ggacagcccg gcccgtccct
     5101 ccaacagatc gccgatgtcg gtggccagcg cctcggcccg ccagccggtg gccagacggt
     5161 tgtccggatc atcgcgcagg aagccctcga ggtccccccg ggtggccagc accgcgggat
     5221 cgagctcgag ctggcgggcc cgctgggaca cccaggacat gagcagcggc acggcaccac
     5281 ggagctcggc gggcacggga cggtcctcgg ggtacacgac gtcctcgggg tccagcgcct
     5341 tgccggctgc cacggcgtcg aggacgtcgt cgcggatgga cttggggatc ccgcggtcgc
     5401 gcagaccccg gatcttgacc aggtcctcga ccgtcttcgg cgggcgctgg gcgatcccgg
     5461 cgagcgccag gtcgggcagc accgagcgca gcggacggtc gacctcggcg gcgtgggcct
     5521 cgcgccacgc agcgagcgac tgggccactc ccgccgccgt gcccttgagc cgacgggcct
     5581 ccttcacccg ccaccaggcc atggccgggt cgctcggagc gagggagcgc tcgaccatcg
     5641 cctcgcactc gctgcggacc caacccatcc ggccccgctg ctccagcgag gccgtcatct
     5701 ggtcgtggag ctcgaagagg tgcacgacgt cggactgggc gtagcgcttg gcgtcctcgg
     5761 agagcgggcg agccagccag tcggtgagcc ggtcggcctt gggcaggtgg atgccgagca
     5821 tcttgttgac gagcggggcg agcccgatgc tgctcatccc gaggaacgac gcggcgatct
     5881 gggtgtcgaa caggtcgtcg gggacgacgc cgacggagtg ctggaagacc tcgaggtcct
     5941 ggcccgccgc gtgcatcacg atcgtgcgac cggtgagcag cgccccgagc ggggtgatgt
     6001 cgacggccag cggatcgacc agcgcctcgc cgtcgcccca gccgatctgc acgagcgcgc
     6061 actgcggcca gtaggtgcgc tcccggtgga actcggtgtc gatggccagg cggggtgcgt
     6121 cggccagctc ggcgaccagc tcggcgagtc cctcgccggt gtcgatccag cgcgccgtcg
     6181 cgggctcagc caccctgttc gaccgcgttc cccgcctcga cccaggcgag cgtgccgccc
     6241 gcgacgttga cggcgtcgac cccgttggcc cggaggtgct cgaccgcctt gccgctgcgg
     6301 ccgccggagc ggcagatgac gtagaccgtc tcgtccgtgg ggaactcgcc cacccgatcg
     6361 ggcacctcgc cgagcggcac gaggcgggcg ccgggcgcgt gcccctgctc gtactcgtcg
     6421 ggttggcgca cgtcgaacag cggtgcgccg tcgtcgagct tctggcggag ggcggtgatg
     6481 tcgatttcgg ggacctgcac ggttccgatg ctaggagttc ccgcgccgga tggcgggctc
     6541 ggcaggtcct cggggcggtc ggcgtcgtgc acggccgcag gtgcgacgtc gtcgagggtc
     6601 gcgatccgca gcgacagggc ggcgccgacc agcgagcgct cccctgccgc gaacacgtcc
     6661 gccaggatcc cggcggtgcg ccggtcccag gccccgtgca gccactggcg acggccctcg
     6721 acgatgggga ccacggcatc gcaagccgcc cgggcccggt ggtcgacgag acgggcgatg
     6781 gtcgccgggt ccgggtcggt gaggtcgcag gccaggacca cggccacccc ggcggagccg
     6841 tcggcatcgt cgagcgcggt gatcaacccc ccgagcgggc cttcgcccgg ccagcggtcg
     6901 ggcatgacgc gcaggccgcg accggtgagc gcctcggcgt cgccgccgac ggtgacgact
     6961 cgatcggccc cggccgcccg gagcgcgtcg gcgacccggc gggccatggc ggtcccgtcc
     7021 acctcgacga gggccttgtc ccggcccatc cggcggctcg cgccaccgca gagcacgtag
     7081 ccgtcgaagc ggccggcgac cacggtcagc cgttccggat gcgggcgagg tcgtcgaggt
     7141 cggcctggtc gaagtcccgc ggcggcccgc cgctgaggac gctgcccagc acggtcgact
     7201 cgaccgcgta gtacatcacc gagccccggt tcgggctgtg gccggggtgc tcggggtcct
     7261 ggcgcccggt gtcgaggacc agatcgacga gcccgagcag gtggccgacc tcgtgcatgg
     7321 tgacggcgtc ctcgacggcg cccgggttgc cgagcatgcc ggatgcctcg tccacccgct
     7381 cggcgaacac ggccgccacg tccccccgca ccgccacgcc gacggcccga tcgttctcgg
     7441 cgaagccacc gtgcaggaac aggacggtga gcaccccctg ctcggcggtc tgggccggcg
     7501 acgatccgtc ggcgatcgcc cgcagctcgt ccgcggtcca cgaccgggcc ccgccaccga
     7561 cggcaccgac cgagacggcc accggcttgc cgctcagctg ggcgagcacc tggcggaccc
     7621 gctccggcgc ctgggtccgt gggccggcac cgtcctgggc gagcacctgg acgaggatct
     7681 gctccgctcc ggccgggcgc aggtaccagc cggccaggga cccgaccgga ccggctgcgg
     7741 ggaccggctc tgcgtcgcca ccgggcgtgg tctcacccgg ggcgggcgca ccggacgcgt
     7801 cgggtgcgct cgcggcgggc ggggcggtgc cctggggggt gccctcgacg tccgacgccg
     7861 gctgctcgtc ggctggtgcc ggctgcccgg gtgtcgacgc ctgcggcgcg gtcgtcgtcg
     7921 tcgaggtgac cgccagctgc tcctccgcgt cggtgccgca cgcggcggcc accaacaggc
     7981 cggtcagcag caggaggacg aggcggctgc gccggaccgg tgcgggcacc gcggcctacg
     8041 acggccggcc ggccgggtcg agctgggcga ggaactgggc gcagcgctcg gcctcgtcgg
     8101 tctccccgat ggcctcggcc gcccgtcgca gcccgtcgag cgagcggagg aaccccgtgt
     8161 tctcgggatg ggtccagcgc acgtagcccg agccccgcca gccgttggcg cgcaggcggt
     8221 cgagaccacg gtggtacccg acccggtagg cggcgtaggc ctcgatccgg tcgcgggcgc
     8281 cgtcgccgag cgccgcccac gcgtgcaggc agcgggggtg gtcggccacg acggcggcga
     8341 cggcacggcg gcggtcgtcc tcggggaggt cgctcgccgc ggccaggcgc tccagcgcgt
     8401 cggccggctc gggatccagc acggtctcag gaggtccgct ggcggacagg ttcaccgatt
     8461 gcgacatggc ccgagcctac ggcccggggg gatgcaccga tccgcgcagg tcccgacgta
     8521 cgttgcgtgc cgtgggaacc atcctcgctg ccgctcccta cgaggagttc gtccgggaca
     8581 acagcccggt cctcgccgtc gtcgcctgtc tgatcctggc gctgctggtc atccgcctgg
     8641 tggtcaagac catgacccgg atgatcctgc tgtcgacgct gctcctgatg gtcctgttcg
     8701 tctacgtcga gcgcgacaac atccaggagt gcacccagac ctgcgagtgc gccatcgccg
     8761 gcatcgacgt cgagctgccg gcgtgcgacc cggcccgcat ctagccctcc gggaccagat
     8821 cgaggtcggc caccaaccgg cgcaccatgg cggccaccgc cgccgggtgg gtcaggtgca
     8881 ccccgtggcc tgagccctgc accacctcga ggtcggcgcc cggcacggtc tcggccaccg
     8941 cccgggtggc ctcgccgtgg tgggctgcgc cctcggtgcc gtgcgcggcg agcaccggca
     9001 cccggatcga ggtgagctcg aagggtggcg ggtgcggcgg tcggagctgg gccagctcgg
     9061 cgaccagggt cggcccctcg gcgcgccggg cgtcccgggt ccggcggggc agccgctccc
     9121 atcgctggtc gccgaccatg cggcgcatga acttctcgcc cgcgtcctcg gggtcgtcgg
     9181 ctgacgcgac cgcggcagcg ccggcgctgc cgctcggcca ccagtccatc cacggcaggg
     9241 gggcctcgaa cgccacgaca ccggcgagcc gctccggggc ctccgccgcg gcggcgaggg
     9301 ccaccgtccc gccgtaggag tggccgaaca ccaccggggg cggaccgtcc cccaccagcc
     9361 aaccgatcac gtcgagcagg tcatcgacct gctggtggaa cgcctcgggc ggtccgaggg
     9421 ccaccgatcg ggcgtagccg cggcggtcgt agcgggccac gtgcatgtcg gacagcgcct
     9481 tgcggacgcg accgaagctg gacgagcgat ccatcgtccc gtgcaccagc acgacgcccg
     9541 ggccctcgcc ggcggcgtcg cgccagcacc gaccgtcggc gacgaagtcc tcccccgtca
     9601 cgtgacgcgc acgacgcgca gcacgtcggt ggtgccgatg tcgtcggcgg tgccggccag
     9661 cacggcgacg atgttgcccg ccttgatgta cccggccttc accgacttct cgaccgcgtg
     9721 ccacaccatc tcgtcggtgg tgctgtactc gtccatcttc agcggcttcg tgccccacac
     9781 gagcgacatc ttgcgcacgg tgtcctcgaa ggggctcgca ccgatgagca cgccgggcgg
     9841 gcggaaccgg gagatggccc gggccgtgta gcccgagttc gtgcaggaga tgatcccgtc
     9901 gagctcgagc tcggtggcga ggcgccaggc ggcgtggctc ataccggcgg tgatgcgcgt
     9961 ggtgaggtcg agctgcacct ggccctggat gcgcccgagc tggctgcccc aggtgctgta
    10021 gtcggcggcg gcctcggcgc gctcgcacag gtgggacatg gtcttgatga ccagggtcgg
    10081 gttgtgcccg atggcggtct cggccgacag catcaccgcg gtcgtcccgt cgaacacggc
    10141 gttggccacg tcggtggcct cggcccgggt cggcgacggt gagtggatca tcgactcgag
    10201 catctgggtc gcggtgatga ccgggacccc gaacgacacg ccctggcgga tgatctgctt
    10261 ctgcaggtgc gggacctcgg cgatgtcgca ctcgttgccg aggtcgccgc gcgccaccat
    10321 caccgcatcg gcgatgttga cgatcgcgtc gagggcgtcg accgcggcct tggtctcgat
    10381 cttggcgacc acgtcggcgc cctgggccag cgccttcacc gcagcaacct cgtcggccga
    10441 gcggacgaac gaggcggcga cgaagtccag ctcgccggcc aggccctcga gcagcgcgat
    10501 gtcctgatcg gtgggcaccg acacgcgaag cttcgctgac ggcagcgtca cgcccggacg
    10561 gccctggatg cgcccgccgt gctcgacctc gaccatgagg cggctgccgt cgttctccac
    10621 cgccaccagc cgcaccgagc cgtccccgag cacgaggctg tcgccgaccg cgaccgcttc
    10681 gagcaggtgg gggtagtcga cgtggatgtg gtccggtgcg gtcggggcgt cacccgggcg
    10741 gatctcgatg cgctggccct cggcgaggaa cagcccaccc tcctcgatga ccggccccat
    10801 gcggatcttg ggtccgggca gatcgcccag caccgagatc gggcggttca tctccgaggc
    10861 gaccttgcgg atgcgcttga tccgcagctg gtgctcctcg atcgtgccgt gggccaggcc
    10921 gaggcggcac acgtcgacgc ccgcgagcat catcgctcgc agcacctcct cggagtcgga
    10981 ggccgggccg atggtggcga cgatcttggt tcgacgggtc acggaacgac actaccgagg
    11041 ccagactggg ccgatggccg gcgacgacga cctcgtgacc tcccgggggc tgcgcgtccc
    11101 gtcgtcggcg atccgctggt gggccagccg gtcgggcggg cccggtggcc agcacgccaa
    11161 cacctccgac acccaggtca ccgtcgagct cgacctcgac gcggccgacc tgcccgaggg
    11221 cgtcacggcg cgcctgcgca ccgcccacgg cgacgcggtc cgggcatcgt cgtcggactc
    11281 acggtcccag taccgcaacc ggcagctggc ccggaagcgc ctcgccgacc tggtggacgg
    11341 cgcggccaag ccgccggccc gacgtcgccc gacgaagccg tcccggggtg cgaagcgccg
    11401 ccggctcgag gccaagcggc agcagagcga gaagaaggcc cgacgctcct ggcgcccgga
    11461 gcgctgaagc gttcgcgccg cgcagatcga ccccaggagg tcgatctgcg cgtcaggaac
    11521 ggatcagctg cggggaacct cggaccaggg cttgtccttg tcgacccgga tgtcgccggg
    11581 gaggccgagg acgcgctcgc cgaggatgtt cttcatgacc tcggtggtgc cgccctcgat
    11641 ggagttggcc cggctgcgca ggaacgactg ctgggtggag ccaccgaaca tgccgacgcc
    11701 gccggtgcgc tccatgtcgt aggagccgta gagcatcccg tgggcgccca tgcagtccac
    11761 ggcgaagctc atgatgtcct tgttgagctc ggccatggcc agcttggcga cggagccctc
    11821 ggggccgggg tcgccgagct tgcgcatctg gctggcccgg atgttggtga ggcgggacac
    11881 ctcggcgcgc acccagagct gcatgagctc gtccttgacg gcaccgtcct cggtgtcgca
    11941 gtgctcctgc caggccttct tggcgatggc gatggggccg gactcgcggg gcgggatcgc
    12001 gccgccgatg gagacgcgct cgttcatgag cgtggtgatg gcgacgcgcc agccctcgcc
    12061 gacctcgccc aggcgctcgg cgtcggggac acggacgtcg gtgaagtaca cctcgttgaa
    12121 ctcggcgtca ccggtcatct ggcgcagcgg ccggacctcg acgccggggg cctgcatgtc
    12181 caccacgaag tgggtgaggc cggcgtgctt gcccgcctcc gggtcgtggc gggccaccag
    12241 ggtgccgaag cgggacacgt gggcgagggt ggtccacacc ttctggccgt tgatgatcca
    12301 ctcgtcgccg tccttgaccg cacgggagga caggccagcc acgtcggagc cggcgccggg
    12361 ctcggagaac agctggcacc agatctcctc gccggtgaac agcgggcgca ggtagcggcc
    12421 cttctgctcg tcggagccgt gggtgtagat ggtcggggcc gacatgccgt gaccgatcgg
    12481 gttccgcccg gcggcgttcg gcgccccggc ggcggccagg cgctcgttga tcaccttctg
    12541 gaagcgggcg gggaggccga ggccgccgaa gccctcgtcg tagtgcaccc acgccaggcc
    12601 ggcgtcgaac tgggcgccga ggaagtcgac cacgtcggtg tccttcggcg ggtgcgcctc
    12661 gaggagcgag tcgatggcgt ccaggacgcg ctgttcgtcg ggatcggtga cggtgggggt
    12721 gcgttcggtg acggccatgg cctgatcgta gtgcacttga ccgagcggtc aactttcgtc
    12781 gaggccggcc tcctcgagcc gttccagcaa gcggtcgcgg accgacaccc ccggcgtgcg
    12841 ggccttctcg gacgcgaccc gccagccgtg gcgggaggag tagacgagcg ggtccgcctt
    12901 gaggcgcccc ggcccgacgt cgcggacgtc gaccacgtag cagtcgtggt cgaggccgag
    12961 gtcgagcttc tcccggatct cgcagcgcag ccacgccagg cagtccacga cgtggggcac
    13021 gggcccgaag ccgtccacct cggtctcggt gaggtactcg tcgaacaggc ggtggaactt
    13081 gcggcccggg tacgagaagt actgcccctc ctccacctgg tcgccggcca ggatcgagac
    13141 gctgaaccag ccggccgcct cgatgagggg gtgcgtgtcg tgcttcgggg acaccgaggc
    13201 gagcagcacc ggctcctcga agctgatctg ggagatccag tgggaggtgt aggcgcgggt
    13261 caccccgtcg tggcgggcgg ccaccacctg cacccccttg gtcatctggc ccaggcactg
    13321 cttcacgtcg cgatcgatca cgacccggag ggtagggccg ggcgatcgct acccgaaggc
    13381 ggccgagagc aggtcggcga agacctcctc ggggtcgtcc tcgatccccc ggcgggcgaa
    13441 ccacgccgcg acgttgcaga cgtcgcggtg caggaacgag agaccgcccg ggttggcggc
    13501 gatgtcgacg gcctggggca ggtcgatgaa ccagacctcg ccgtcccacc agagcaggtt
    13561 gtaggccgac agatcgccgt gggcccagcc ggccgcggtg atgcggcgca ggccgtccac
    13621 cacgtcgtgc cacgcggcgc gcacctcgtc cggcgtcagg cgcgcggcgg cgagctgcgg
    13681 ggccgcctgc tcgaggtcgc cgacgtactg catgaggagc acgtcgtcgg cgtaggagac
    13741 cgggtacggc acgcggcaac ccgcgtcgtg gagccggccg aggacgtcgt gctcgtggtc
    13801 gagccagcgg tcctgcagga gcttgcggcc gtaggtcgtc atggtctcga cggcgcgccg
    13861 gtcgcggctc ttgcggaact ggcggccctc ccggtactcg acgtcgtggc ggaaggcgga
    13921 cgagcgctgc accccgagcg cctccagctg gcccttcgac gtgacggtgc ggggcaggta
    13981 gcgcttgcgg gcgagcaggc aggtccggcc atcggcgccg acgcgctcga tgacgtcgat
    14041 ctgggcctcc ttgccggagc gcagggtgcc gaggtcgtgc tcgacgacgg gatcgtcgac
    14101 gagccaggcg gggatcgcgg tggacgacgt ggcgcggacg gtacgaccgg gtgcgccggc
    14161 gacccagcgg ttttcagttg aggcgggccc gcagccggcg ggccttggcc agctcgtcgt
    14221 cgggatccga cgtgtggccc tcctcgacca gggactccag caccttggcg gcccggacga
    14281 agtcaccgca ggcggcgagg gcgcccccgt aggcgcgccg gtcatcgggc gtggatcccg
    14341 gcaccaatgt ccgcaggcgc gacgcccaca gcagcgcggt gcggtcgttg cgctggcggt
    14401 ggatggcgac caggttgccg agcatccgag cgacgatcgc ccggggcggg accgggtcga
    14461 ggtacgcgtc gaggtcgatg cccggcgcga ggcgggagaa ctggtcgtcg agctcgtcgc
    14521 ggtcgaggat gcgcccgccg gcgaacgcgt cgacccagcg gcgcgtgccg gtggtggtgc
    14581 aggtcatgaa gtgggcgggg gtgccgacgc ccgcgagcgg cacgccagcg cggcgaccga
    14641 cctcgatgag cgcgacggag agcgtgatcg ggatgccccg ccggcgctcg agcacctcgt
    14701 gcaggtagga gttgcgcggg tcgtagtagt cgacctggtc gcccacgaac ccccagcggc
    14761 cgaagagcga cgtggtgagc gcgggcaggg tcggctccgg caccgagcgg gccagctcgt
    14821 cgagccgggc caggttggcg gcgacgtcgc agcccggatc ggcgtgcacc gcgatgagca
    14881 gcgcggcctc gtcgaggggg aagtcgggct gggacaccag gtccgcgaaa cgctccgtca
    14941 cctccacggg ggcgacggta gccccctgcc gccaggcgtg cgccgggaac cgacaggtgc
    15001 cccgggcggg gcggtccggc cccgcggcta cggtctgcgg cgcgccccgg cgcacgttct
    15061 tgccccttgt tccaggagac cgccaccaca tgtaccccgg tgcattcgcc gcaacccggc
    15121 ccgaccaccc ggccgtgatc atggccggca cgggtcagac catgacctac gccgagctcg
    15181 acgaggaggc caaccggctg agccagctgc tccgctcggt cgggctccag ccgggcgacc
    15241 acgtggcgtt ctgcatggag aaccaccccc ggttcctggc cgtggcatgg ggctgccact
    15301 acgccggcct ctactacacc gccatgtcgt cgcggctgaa cgacgacgag atgcagtaca
    15361 tcctcaacga ctgcggcgcg aaggccttca tcaccacggc ctacaagcgc gaggcggccg
    15421 agaagctgct gggccaggcc cccgacgtgg agacctggct gatgatcgac ggcaccgtcg
    15481 acggctacga gtcctacgag gacaccatcg ccgcccagcc cgccgagccg ctcgagggcc
    15541 gcgtcgccgg cgccgacatg ctctacagct cgggcaccac cggcaagccc aagggcgtca
    15601 agcccccgat gcccagccag ccgctggagg aggccggcac ctcggtgtac ggcctgatgg
    15661 cgatgctgtt cgggctcacc gaggacagca tgtacctgtc ccccgcgccg ctctaccacg
    15721 ctgccccgct gcgcttctgc atgggcgtgc acatgagcgg cgccacggtc gtggtgatgg
    15781 agcgcttcga ccccgaggag gcgctcgagc tcatcgagca gcatcgcatc acgctgagcc
    15841 agtgggtgcc gaccatgttc atccgcatgc tcaagctgcc cgaggacgtg cgcgccaagt
    15901 acgacgtctc gtcgctgcag gtcgccgtcc acgccgccgc gccgtgcccc gtcgaggtca
    15961 agcgccgcat gatcgagtgg tggggcccgg tcctgcacga gtactacgcc ggcaccgagg
    16021 gcaacggctt cgtgtactgc aacagcgagg actggctgga gcaccccggc tccgtcggca
    16081 aggcgctcat cggcaccatc cacatcgtgg gcgaggacgg cgaggagctg ccggtcggcg
    16141 agccgggcac cgtctacttc gagggcgaga acgcggcgac gttccagtac cacaacgatc
    16201 cggagaagac cgaggactcc cgcgacccca agggccgggg ctggtccacg ctcggcgacg
    16261 tcggccgcct cgacgaggac gggttcctct acctgaccga ccgcaaggcg tacatgatca
    16321 tctcgggtgg cgtgaacatc tacccgcagg aggccgagaa cgtcctggcc atgcacccca
    16381 aggtgtacga cgtggccgtg ttcggcgtcc ccaacgagga cttcggcgaa gaggtcaagg
    16441 ccgtggtcca gccggccgag ggcatcgagc ccacccccga gctggagcgc gagctcatcg
    16501 ccttctgccg cgagaagctg gccgacgtga agtgcccccg ctcgatcgac ttccgcgacg
    16561 agctgccccg ccaccccacc ggcaagctct acacgcgcct gctcaaggac gagtactggc
    16621 agggccacga gtccggcatc atctgagccg cgaagcgacg acccgcgagc gcccgtagac
    16681 gacgccggcc gcggccagcg ccgaggccac gccggccaac aaccagccga ggccgtagcc
    16741 ggccgagtcg aagaccccgc cgaacaggat cggcccggtc agcgcgccca cgtacacccc
    16801 ggtctgggtg atgccggtgg ccgcggccgg tgccgtcggg ttcagccgca cgacg
//
LOCUS       DATISU010000047         3620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_235036, whole genome shotgun sequence.
ACCESSION   DATISU010000047 DATISU010000000
VERSION     DATISU010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235036"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VLR27_02215"
     CDS             complement(<1..857)
                     /locus_tag="VLR27_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02284.1"
                     /translation="MLPEPAIDLLRAQYGLLAWHQLPKDLTAADRRRVGRHPDLERLS
                     PRVLRHRVVRPCREQHVLAGVLDAGVGGQLWGKSASSLWGFGRFRSSRPHVAVHRTLV
                     RGERLAQIHLVRHHIAAATTHLGIPTARPEETVLWIAGMWTHRWGPGGIEIAIQRTGR
                     TLDHAWRLGLIDGQRIHELCAASGGRGRSGIVVLRQLLDERPPDYRPSGSALEDRFES
                     TLPKSLRAELERQVPLGDDHPIGIVDYCHRTRPLVVEINGEVFHSSLTDRAADEQRYR
                     RLVAAGYEVL"
     gene            986..2155
                     /locus_tag="VLR27_02220"
     CDS             986..2155
                     /locus_tag="VLR27_02220"
                     /EC_number="1.1.1.306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050607 - mycothiol-dependent formaldehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010127 - mycothiol-dependent
                     detoxification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HSP02285.1"
                     /translation="MTTRRHERRPERIGGRFPRTDEVEETAVVHEVRGVVARGKGAPV
                     TVETILVPDPGPGEALVQVQACGVCHTDLHYREGGINDDFPFLLGHEAAGVVEAVGDD
                     VTSVAPGDFVVLNWRAVCGECRACERGQPHLCFDTHNATQKMTLADGTELSPALGIGA
                     FADKTLVAAGQCTKVNPDADPAAAGLLGCGVMAGYGAAAHTGQIGEGQSIAVFGCGGV
                     GMAALAAAKLRQATTIIAVDLDPKKLELAKSFGATHTVNPNDGDPVEAIKSLTGGFGA
                     DVCVEAVGNPKVLEQCFYARDLAGTVVQVGVPTPDMKFPDIPMIDFFGHGGALKPSWY
                     GDCLPSRDFPALVELFLQGRFPLGDFVSERIGIDDVEEAFHKMERGEVLRSVVVL"
     gene            2155..2787
                     /locus_tag="VLR27_02225"
     CDS             2155..2787
                     /locus_tag="VLR27_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSP02286.1"
                     /translation="MAPRIEKVVTSGIFSLDGEDHEVDNNIWLYGDDDEVVVIDAAHD
                     HRPILAAVGDRKVVAIVLTHGHNDHINAAADLADATGAPMLVHEDDRMLWEVVYPHRG
                     PNGTLTDGDTVTVAGESLRVLHTPGHSPGGICLFTDPDGANPGHVFGGDTLFNGGPGA
                     TGRSFSSFDTIIESIKTKLLTLPPETVVHTGHGDDTTVGAEAPGIEAAAN"
     gene            complement(2864..>3620)
                     /locus_tag="VLR27_02230"
     CDS             complement(2864..>3620)
                     /locus_tag="VLR27_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSP02287.1"
                     /translation="GWLVPHLADRYRVVRLDFRGHGRSGRTPGAYRFPDYIADATAVC
                     EQVLGQPAVVIGHSLGGGTAAGLAQTRPELVRGVVLEDPAIMAPPVAAATESAPLEGN
                     SLLDGFRLMRESIPRLQESGISAADLSATLRDAPSPSGPTFGELVTDDALDAMAEAML
                     QLDASVLDPVFDGSHAPVFDPEQALPVPGIVIAGDPELPDTLVREADLARLAARSPEV
                     ESRVADGCGHLIHDSRQHRAIVLAAIEDLLARL"
BASE COUNT          545 a         1252 c         1341 g          482 t
ORIGIN      
        1 agcacctcgt agccggccgc cacgagtcgg cggtagcgct gctcgtcagc agcccggtcg
       61 gtgagggacg agtggaaaac ctcgccgttg atctccacga ccaacggtcg agtgcggtgg
      121 cagtagtcga cgatgccgat ggggtggtcg tcgcccagcg gcacctggcg ctcgagctcg
      181 gcacgcagcg acttcggcag cgtcgactcg aagcggtcct ccagcgcaga gcccgacggt
      241 cggtagtccg gggggcgttc gtcgagcagc tggcggagga ccacgatccc cgaccggccc
      301 cggcctccac ttgcggcgca cagctcatgg atccgctggc cgtcgatgag cccgagtcgc
      361 caggcgtggt cgagcgtgcg gccggtgcgc tggatcgcga tctcgatgcc gccaggcccc
      421 caccggtggg tccacatgcc ggcgatccag aggacggtct cctccggtcg cgccgtcggg
      481 atgccgaggt gcgtcgtggc ggcagcgatg tggtgcctga cgaggtggat ctgggcgagc
      541 cgctcgcccc ggaccagcgt gcgatgcacg gccacgtgag gacgcgacga ccggaagcgt
      601 ccgaaccccc agagcgacga cgcgctcttg ccccagagct gcccgccgac tccggcgtcg
      661 agcacgccgg cgaggacgtg ctgctcccgg cacggtctga cgacgcggtg ccgcaggacc
      721 cgggggctga gtcgctccag atcggggtgg cgaccgaccc gccggcgatc ggcagcggtc
      781 aggtccttcg gcaactggtg ccaggcgagc aggccgtact gggctcgcag cagatcgatt
      841 gccggttcgg gcagcacgtc gtcgcctccg cgatctcgcc cccgcaaggg gagggaggag
      901 cgacatgcac cggccactcg acgtggggcc gaccgtaccg agccccaccg acgggaaccc
      961 cccaggaacg ttctggccta catccatgac caccaggcgg cacgaacgta ggccagaacg
     1021 gatcggcggt aggttcccgc gcaccgacga ggtggaggag acggccgtgg tgcacgaggt
     1081 ccggggagtg gtggcgaggg gcaagggtgc gcccgtcacg gtggagacga tcctggtgcc
     1141 ggatcccggg ccgggggagg cgctggtgca ggtgcaggcc tgcggggtgt gccacaccga
     1201 cctgcactac cgggagggtg gcatcaacga cgacttcccg ttcctgctcg ggcacgaggc
     1261 cgccggcgtg gtcgaggccg tcggtgacga tgtcacctcc gtggcgccgg gcgacttcgt
     1321 ggtcctgaac tggcgggccg tgtgcggcga gtgccgggcg tgcgagcggg gtcagcccca
     1381 cctctgcttc gacacccaca acgcgaccca gaagatgacg ctggccgacg ggaccgagct
     1441 gtcgccggcg ctcggcatcg gggcgttcgc cgacaagacc ctcgtcgccg ccgggcagtg
     1501 caccaaggtc aaccccgacg ccgacccggc cgccgccggc ctcctcgggt gcggggtgat
     1561 ggccggctac ggggcggccg cccacaccgg tcagatcggt gagggccaga gcatcgccgt
     1621 gttcggctgc ggcggcgtcg gcatggccgc cctcgcggcg gcgaagctgc gccaggcgac
     1681 gacgatcatc gccgtcgacc tcgacccgaa gaagctcgag ctggcgaagt ccttcggcgc
     1741 cacccacacc gtgaacccga acgacggcga cccggtcgag gcgatcaagt cgctcaccgg
     1801 cggcttcggt gccgacgtgt gcgtcgaggc ggtcggcaac cccaaggtcc tcgagcagtg
     1861 cttctacgcc cgggacctgg cgggcacggt ggtgcaggtg ggcgtcccga caccggacat
     1921 gaagttcccc gacatcccga tgatcgactt cttcgggcac ggcggcgcgc tcaagccgtc
     1981 gtggtacggc gactgcctgc catcccggga cttcccggcg ctcgtggagc tgttcctgca
     2041 gggccggttc ccgctcggcg acttcgtgtc ggagcggatc ggcatcgacg acgtggagga
     2101 ggcgttccac aagatggagc gaggcgaggt cctgcgatcg gtggtggtgc tgtgatggcc
     2161 ccccgcatcg agaaggtggt cacgtcgggc atcttcagcc tcgacggcga ggaccacgag
     2221 gtcgacaaca acatctggct ctacggcgac gacgacgagg tcgtggtgat cgacgcggcc
     2281 catgaccacc gtccgatcct cgcggccgtc ggcgatcgca aggtcgtggc catcgtgctg
     2341 acccacggcc acaacgacca catcaacgcg gccgccgacc tggccgacgc caccggtgcc
     2401 ccgatgctcg tgcacgagga cgaccggatg ctgtgggagg tcgtctaccc ccaccgcggc
     2461 ccgaacggca cgctcaccga cggcgacacg gtgacggtcg ccggcgagtc gctgcgggtg
     2521 ctgcacacgc ccggccactc ccccggcggc atctgcctgt tcaccgaccc cgacggcgcc
     2581 aacccgggcc acgtgttcgg cggcgacacc ctcttcaacg gcggtccggg tgcgaccggc
     2641 cggtcgttct ccagcttcga cacgatcatc gagtcgatca agacgaagct gctgacgctg
     2701 ccgcccgaga cggtcgtgca caccggtcac ggcgacgaca ccaccgtcgg cgccgaggcc
     2761 cccggcatcg aggccgccgc caactgatcc cgtcgttctg gcctacgtcc atgaccacca
     2821 ggtggcacgg atgtaggcca gaacggatct cgggttgggc gggtcagagg cgggccagga
     2881 ggtcttcgat cgcggccaac acgatcgccc ggtgctggcg ggagtcgtgg atgaggtgtc
     2941 cgcagccgtc ggcgacgcgg gactcgacct cggggctgcg cgccgccagg cgggcgaggt
     3001 cggcctcgcg caccagcgtg tcgggcagct cggggtcacc ggcgatgacg atgccaggca
     3061 ccggcagcgc ctgctccggg tcgaagacgg gcgcgtggct gccgtcgaag accgggtcga
     3121 ggacgctggc gtccagctgc agcatcgcct cggccatggc gtcgagggcg tcgtcggtga
     3181 cgagctcgcc gaacgtcggg cccgacgggc tgggtgcgtc gcgcagggtg gcggacaggt
     3241 cggcggccga gatgccggac tcctgcaggc gggggatcga ctcccgcatc aggcggaagc
     3301 cgtcgagcag cgagttgccc tcgagcgggg ccgactccgt cgctgcggca acgggcggcg
     3361 ccatgatcgc cgggtcctcg agcacgacgc cccgcaccag ctccgggcgg gtctgggcca
     3421 ggccggccgc ggtgccgccg ccgagggaat ggccgatcac gacggccggc tggccgagga
     3481 cctgctcaca gaccgcggtg gcgtcggcga tgtagtcggg gaagcggtag gcgccgggcg
     3541 tgcgacccga gcggccatgt ccccggaagt cgaggcggac cacccggtac cggtcggcca
     3601 ggtggggcac gagccacccc
//
LOCUS       DATISU010000048        13864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_421431, whole genome shotgun sequence.
ACCESSION   DATISU010000048 DATISU010000000
VERSION     DATISU010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 13864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13864
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421431"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1799)
                     /locus_tag="VLR27_02235"
     CDS             complement(<1..1799)
                     /locus_tag="VLR27_02235"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF004531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HSP02288.1"
                     /translation="MTRWVYGFDEIDAARAAVDDDWDRVRGLLGGKGANLADMASLGV
                     PVPPGFTITTEACNAFIAGDGTLPEGLWDEVLAAVRAMEEGTGKTYGGTHNPLLVAVR
                     SGAKFSMPGMMDTVLNVGLNDEVAAAMIERTGDERFVLDSHRRLVQMFATVVLGMDGD
                     PFEEILREHRGTHGVINDADLPPEALRATVDAFRAHVRARSATPFPEDPYDQLRLATE
                     AVFHSWNSRRAFDYRRAAKIADDLGTAVNVVAMVFGNTGMNSGTGVLMSRNPTNGEAK
                     LEGDYLLNAQGEDVVAGNRETKPIEQLAEDLPDAAEELGAIARRLEEHYREMQDMEFT
                     VEDGTLWLLQTRTGKRTAQAAVRIAVDMAGEGLITKAEAVGRVTPDQVDFFLHPQFSS
                     ESLAGLTAVAQGLNVSPGAAVGVVAFDPDLAERWGREGRDVVLVRHETKPDDVHGMLA
                     ATGILTSSGGRTSHAALVARQFGKPAVTGASAIDIDMASRTLTVGNDTLHEGEWISID
                     GTSGNIYLGKVETVAPDLDNEWLTTVLGWADEFRTLGVRANSDEPEDAARARSYGAEG
                     IGLCRTEHMFFDPERLPIVQRMITAPNLAERKEA"
     gene            complement(1847..2836)
                     /locus_tag="VLR27_02240"
     CDS             complement(1847..2836)
                     /locus_tag="VLR27_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP02289.1"
                     /translation="MTTAMLPDDLDPILRDGFERHRRELTGYCYRMLGSPSEADDAVQ
                     DTLLKAWQAYDRFEGRSSLRSWLHRIAHNVCMDMHRSPQRRARPMEMGPSTKVDDVVL
                     GDWREETLFVQPVRDEEVLDLGGDPADVAAARESIRLAFVAALQHLPAKQRSALILCD
                     VLRWPAAEVAELLETTVASVNSALQRARATLEQRRDEPLDPLVDPDHQSLLARYVEAF
                     ERYDIPAVVALMREDAVLSMPPWDTWLEGRDEVGAWMLGQGIGCKDSTLIPIDVNGTA
                     GFAAYKPGDDGRRHPWAIQVIEVADGRIVGHHNFIGPELFARFGLPEHLSGDD"
     gene            2910..4499
                     /locus_tag="VLR27_02245"
     CDS             2910..4499
                     /locus_tag="VLR27_02245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSP02290.1"
                     /translation="MGNESDGAAAPAEIEALLRAHPGAVVAAVGPDGLFVPLPDELDL
                     GADHHHTGLRSATDLVEGPDRVAVIAAWERARAGGDDSVEAQLRDHTAAGKMHFVDAR
                     DVWGVHIMVFAVDDGGGRVTVPESPPIPEGSRAKVVRSRKDGLAMLIDIDRNVSELLG
                     WRVEDMVGVRSLEFIHPDDQDESIAAWMDMLSAPGLTTRTRLRHSRADGSWVWLDISN
                     RNLLEEEGHVECEMVDVSQEVAAQEALAASEQVLRRLTESMPVGVFHIDADERLQLAN
                     ARLHEILGTRPGDDQATMLSTLRDEMAFDRALSAVLAGTDVDLELEIQVLGTLDVRRC
                     TLALRALTVDGAVTGAVGVLTDVTESARLREELERRATMDDLTGCFNRASVLEALEGF
                     LTTGTDVAVVYVDIDDFKVVNDGHGHAAGDEVLQAIGARLRSAVREHDAVGRMGGDEF
                     LAVCAGLGSEDGAAELAQRIAASLSDPVGFRRQRITVSASVGVAWSPGGEQIDADALV
                     ARADSAMYVTKGAGGGGHTVH"
     gene            complement(4507..5283)
                     /locus_tag="VLR27_02250"
     CDS             complement(4507..5283)
                     /locus_tag="VLR27_02250"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HSP02291.1"
                     /translation="MIRTVDFDTQAILEAVHADGVAVCERVLDAGAIAAIRADLDRVL
                     ETTPTGRNDFEGYGTKRIYNLFGKTRSFDDLAIHPVLLGVLDDLLGHYQLSAPTGIQI
                     GPGEPAQGLHTDDSIYPLPQPHGEVVVNTMWAFDDFTRANGATRFVEGSHRWGPERWP
                     TEDDPVLDAEMPAGSVAFYSGSIWHGGGANATDEPRLGVILEYAAAWLRPQENHVVGV
                     APEVVATLPERLQELLGYNIYPPFVGYVDGRHPKRFLPTP"
     gene            5331..6941
                     /locus_tag="VLR27_02255"
     CDS             5331..6941
                     /locus_tag="VLR27_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HSP02292.1"
                     /translation="MGTWNFADIWETVADELPDAPCLIHGDDRRSWSEVDRRADGVAQ
                     HLLDAGLERQQAVALYLYNCPEYLEGTYAAFKGAFVPVNTNYRYTADELLYLWDNADA
                     GSVVFHGAFVDTIEPIRDRLPKVKAWLWVDDGSGACPDWATPYESAATSGAGRVSPEW
                     GRTGDDLNMLYTGGTTGMPKGVMWRQDDLAVKLTATLGNPLTDDGTVEDIKGTFNEPG
                     ARFLPACPQMHGTGNFPCLSTLSGGGSVVTLTNRTFDPVEMLDAIEREGVNLVALVGD
                     AFGKPILRTLDENEGRWDLSSLLAMVSSGVMWSKETKEGLLRHHPGMMLMDAFSSSEA
                     LGMGSSISGAGATAETAKFELSPETIVIDESNRPIAPGSDEIGRLAVGGRQPIGYYKD
                     PEKTAATFLEIDGQRYSCPGDFATVDADGKISLLGRGSVCINTGGEKVFPEEVEEALK
                     THPAVFDAVAVGVPDEKFGEAVTAVVQVREGADLDEGDVIAHVKTKLAAYKAPKRVVV
                     VDTIGRAVNGKVDYKRLKGEAQERLGVS"
     gene            complement(6934..8622)
                     /locus_tag="VLR27_02260"
     CDS             complement(6934..8622)
                     /locus_tag="VLR27_02260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HSP02293.1"
                     /translation="MKQLTGLDATFLYMETPSTYGHVSGLSIYDRPSPDFDPLQEWRA
                     QIQRRLHLLEPLTRRLRNVPFNLDHPFWIEDPDFDLDFHVRHTAVPRPGRDDQVAALV
                     ARIIARPLDRSRPLWESYVIDGLAGDRFGILTKIHHATVDGASGVELLTIMLDTTPEG
                     DDIPSPQLAPRQPEPVPSDAEVLGMAARTLARKPGRFLVLGARTMRSLGEASRNPILR
                     ASGEQLRDGLRGPLGTVLNLGRSRPEERDEAPQPLPHNAPSTIFNASISPHRSFAYRS
                     VSMESIKAIKGAIGCTVNDVVMAVCAGGLRTYLERRDALPDQPMVSMVPVSIRTGEET
                     ERWSNRVSAIFADLPTDEPDPLQRIRKVNNAMALAKGLHDALPADQLTEFADFPPPAV
                     FARASRMATRFQLGNRTTMPVNITISNVPGPREPLYMAGAKLAHYVPVSTVVEGQGLN
                     ITVQSYCDTLDFGLVGCARLVPDIDVLLDDIIDDIEGLAKLMDVDVPVQLAEARTPGR
                     ATAKKTTPKKATAKKRATKKKAARKKAAAKKTTATRSRTKKAAAKKRPGTGGVS"
     gene            complement(8649..9455)
                     /locus_tag="VLR27_02265"
     CDS             complement(8649..9455)
                     /locus_tag="VLR27_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03084 family metal-binding protein"
                     /protein_id="HSP02294.1"
                     /translation="MDAQGIRADLIAEQDALDAVVADLTAEQWATPTPSPRWTVADQI
                     AHLTYFDGTATLAIRDPDAFNASLGELVELLEGGEEAMDDHTLGWARAASPEDLLARW
                     RENRADLADAATGLSDDDRVPWYGPSMGAKSFLTARLMECWAHGQDVVDALRAAGLDA
                     ERPATDRLAHIARLGFITRGWTYINRGEEPPATPVRVVLDAPGGDTWTFGPDDATESV
                     TGPALDFCLVVTQRRNVDDTDLTVSGDAARDWMHKAQAFAGPPTDGPPAR"
     gene            complement(9553..9732)
                     /locus_tag="VLR27_02270"
     CDS             complement(9553..9732)
                     /locus_tag="VLR27_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02295.1"
                     /translation="MSERTAIDCPDCTDDTEHCHWAWVRHPDGGECLALECRFGPDAH
                     VVVIDCVDVDHGCCP"
     gene            complement(9729..10718)
                     /locus_tag="VLR27_02275"
     CDS             complement(9729..10718)
                     /locus_tag="VLR27_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HSP02296.1"
                     /translation="MPRAIWTGSISFGLVNVPVKLFTATEDRSVGFHQFEPESGERIR
                     YQRVTEQSGKEVEYGSIVKGYELEDGSHVLITPEELEAVEPGKSRTIDIEDFIDLDDV
                     DPVYFDKTYHLAPADQAAAKPYALLREAMKDSNRVAIARFVMRAKQSLAAIRVASSDD
                     GDAGDVLVLETMHFGDEVRSPADIREMQVLDDGIEIKDRERAAAKSLVESLTTDWEPA
                     KYTDTYRERVLDLVARKADGERIVTEKAEEPTASVTDLMAVLEASVNEARQKRGEAPR
                     GASSASASGTDYASMSKDELYEEAQRRDIDGRSKMTKDELVAALSGSAMAEAS"
     gene            10789..12384
                     /locus_tag="VLR27_02280"
     CDS             10789..12384
                     /locus_tag="VLR27_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HSP02297.1"
                     /translation="MTVLRSRLDSTSDEHQANVAAMQALWDEVAEQLASVPSVGGQRY
                     VDRHRRRGKMLVRERIEALIDPYTPFLELSPLAAWGTEDPVGAGVCAGIGIVCGTEVI
                     VTGSDMTYRGGSANPTTVAKNLRIDEISRRNRLPVITLNESAGAELPRQADIFVPGGA
                     GFKNITQRSKLGIPSVTVVFGPSTAGGAYTPGMSDYTVFVKERGMAYLGGPPLVKMAI
                     DEEVDEETLGGAEMHSSVSGLSDYLAADEMDAIRIARDIVAHLRWEKLGPGPSEPADE
                     PLYDPAELIGAASADVRVPFDVREVLARTLDGSRFEEFKPRYGDKLVCGWGSICGFPV
                     GVLANNGILFSEESNKGAQFIQLCNRTDTPLVFVQNITGFMVGSAAERGGIIKNGAKL
                     INAVSNSEVPHLVLMVGASYGAGNYGMSGRAYDPRFVFSWPNHRIAVMGPKQLAGVMD
                     IIARNAAAGRGQEVDEEALAAQTSALENLVEEQSTALYAIGRLWDDGIIHPADSRTVL
                     GIALSATHSAEVRGATDYGVWRH"
     gene            12455..>13864
                     /locus_tag="VLR27_02285"
     CDS             12455..>13864
                     /locus_tag="VLR27_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HSP02298.1"
                     /translation="MIRRLLIANRGEIAVRIARTAHRLGIDTVGVYSTSDADALHVDV
                     VDLAVALGGATPTESYLRAEAVIQAALDTGCDAVHPGYGFLAERADVAHMVVDAGLIW
                     VGPTPEQISLLGDKIAAKRAAVEAGVPTTEAVEVRPGHVPDGLTMPALVKAAAGGGGR
                     GMRIVRSADQLPDAVVAASREAEAAFGDGTVFVEPFIERGRHVEVQIVGDGHGNVIHL
                     GERECSIQRRNQKVLEESPSAGITAETRTALCDGALALARHIGYRSAGTVEFMVGDDG
                     TVSFLEVNTRLQVEHPVTEAVTGLDLVELQLRVAAGEALPLTQDDVRFDGHAIEVRVV
                     AEDPATGWLPSTGTVTRFQIGTAAGADPLETGSAGVAAVRVDTGFRAGSVVSADYDSL
                     LAKVISHAATRSESAAVLARALRSAVVTGVRTNIDAMAALLVEDDFLEARTPTAYLDE
                     HPDVVHAAGPIGDDRVAQLL"
BASE COUNT         1882 a         4845 c         5090 g         2047 t
ORIGIN      
        1 gcctccttgc gctccgccag gttcggggcg gtgatcatcc gctggacgat cggcaggcgc
       61 tcggggtcga agaacatgtg ctcggtgcgg cacaggccga tgccctcggc gccgtaggac
      121 cgggcccggg ccgcgtcctc gggctcgtcg gagttggccc gcacgccgag ggtgcggaac
      181 tcgtcagccc agcccagcac ggtggtgagc cactcgttgt cgaggtcggg ggccaccgtc
      241 tcgaccttgc cgaggtagat gttccccgac gtgccgtcga tggagatcca ctcgccctcg
      301 tgcagggtgt cgttgccgac ggtgagggtg cggctcgcca tgtcgatgtc gatggcggag
      361 gcgccggtga ccgccggctt cccgaactgc cgggcgacca gggcggcgtg gctggtgcgt
      421 ccgccgctcg aggtgaggat gccggtggcg gcgagcatgc cgtgcacatc gtcgggcttg
      481 gtctcgtggc ggaccagcac gacgtcgcgc ccctcgcgcc cccaccgctc ggccaggtcg
      541 gggtcgaacg ccaccacgcc gacggcggcg ccgggggaga cgttgaggcc ctgggcgacg
      601 gcggtgaggc cggcgagcga ctcggacgag aactgggggt ggaggaagaa gtcgacctgg
      661 tcgggggtga cccggcccac cgcctcggcc ttggtgatga ggccctcgcc ggccatgtcg
      721 accgcgatgc gcacggccgc ctgggcggtg cgcttgccgg tgcgggtctg cagcagccag
      781 agcgtgccgt cctcgacggt gaactccatg tcctgcatct cgcggtagtg ctcctcgagg
      841 cgccgggcga tggccccgag ctcctcggcc gcgtccggga ggtcctcggc cagctgctcg
      901 atgggcttgg tctcgcggtt gccggcgacg acgtcctcgc cctgggcgtt gagcaggtag
      961 tcgccctcga gcttggcctc gccgttggtc gggttgcgcg acatgagcac gccggtgccc
     1021 gagttcatcc cggtgttgcc gaagaccatg gccaccacgt tcacggccgt gccgaggtcg
     1081 tcggcgatct tggccgcccg ccggtagtcg aaggcccggc ggctgttcca gctgtggaac
     1141 acggcctcgg tggccaggcg cagctggtcg taggggtcct cggggaacgg cgtggccgag
     1201 cgggcccgga cgtgggcgcg gaaggcgtcg accgtggccc ggagggcctc gggcgggagg
     1261 tcggcgtcgt tgatgacgcc gtgggtgccg cggtgctcgc gcaggatctc ctcgaagggg
     1321 tcaccgtcca tcccgagcac cacggtggcg aacatctgca ccaggcggcg gtgcgagtcg
     1381 aggacgaagc gctcgtcgcc ggtgcgctcg atcatggcgg cggcgacctc gtcgttgagc
     1441 ccgacgttga ggacggtgtc catcatcccg ggcatcgaga acttggcgcc cgagcgcacc
     1501 gcgacgagca gggggttgtg ggtgccgccg taggtcttgc cggtgccctc ctccatggcc
     1561 cgcaccgcgg cgagcacctc gtcccacaac ccctcgggga gggtgccgtc gccggcgatg
     1621 aacgcgttgc acgcctcggt ggtgatggtg aagcccgggg gtacggggac gccgagcgag
     1681 gccatgtcgg ccaggttcgc ccccttcccg ccgaggaggc cgcggacccg gtcccagtcg
     1741 tcgtccacgg ccgccctcgc ggcatcgatc tcgtcgaacc cgtacaccca acgtgtcatg
     1801 ggccgaggtc taccccctcg ggtccgaccg ggtacagggt ccggggtcag tcgtccccgg
     1861 acaggtgctc ggggaggccg aagcgggcga acagctcggg cccgatgaag ttgtggtggc
     1921 cgacgatgcg cccgtcggcg acctcgatga cctggatggc ccacgggtgg cggcgcccgt
     1981 cgtcgccggg cttgtaggcc gcgaacccgg cggtcccgtt gacgtcgatg gggatcaggg
     2041 tcgagtcctt gcacccgatg ccctgaccga gcatccaggc gccgacctcg tcgcggccct
     2101 cgagccaggt gtcccacggc ggcatggaca gcacggcgtc ctcgcgcatc agggcgacga
     2161 cggccgggat gtcgtagcgc tcgaacgcct cgacgtagcg ggcgagcagc gactggtggt
     2221 ccgggtcgac cagcgggtcg aggggttcgt cgcggcgttg ctcgagcgtg gcccgggcgc
     2281 gctgcagggc gctgttcacc gacgccaccg tcgtctccag gagctcggcg acctcggcgg
     2341 ccggccaccg gagcacgtcg cacaggatca gggccgagcg ctgcttggcc ggcaggtgct
     2401 ggagagcagc gacgaaggcg aggcggatgg actcccgggc ggcggccacg tcggcggggt
     2461 cgccgccgag gtcgaggacc tcctcgtcgc gcaccggctg cacgaacagg gtctcctccc
     2521 gccagtcgcc gagcacgacg tcgtcgacct tggtcgaggg gcccatctcc atcggacggg
     2581 cgcggcgctg cgggctgcgg tgcatgtcca tgcacacgtt gtgggcgatg cggtgcagcc
     2641 acgatcgcag cgacgaccgg ccctcgaagc ggtcgtaggc ctgccacgcc ttcagcagcg
     2701 tgtcctggac ggcgtcgtcg gcctcggacg gcgagccgag catccggtag cagtagccgg
     2761 tgagctcgcg tcggtggcgc tcgaacccgt cgcgcaggat cgggtcgagg tcgtcgggca
     2821 gcatcgccgt ggtcatcccc cagagtctgt tcgtccgcgg cgccctccgc caccggactt
     2881 cccactccgc cgacctatgg ttgccggcga tggggaacga gagcgatggc gcggcggcgc
     2941 cggcggagat cgaagcgctc ctgcgcgccc acccgggggc ggtcgtggcg gccgtggggc
     3001 ccgatgggct gttcgtgccc ctgcccgacg agctcgacct cggtgccgac caccaccaca
     3061 ccgggttgcg gtcggccacc gacctggtcg agggtcccga tcgcgtcgcg gtgatcgccg
     3121 cgtgggagcg ggccagggct ggcggcgacg actcggtcga ggctcagctg cgcgaccaca
     3181 ccgccgccgg gaagatgcac ttcgtcgacg cccgcgacgt ctggggcgtc cacatcatgg
     3241 tcttcgccgt ggacgatggc ggcggccggg tcacggtgcc ggagtccccg ccgatcccgg
     3301 agggctcacg ggccaaggtc gtccgctccc gcaaggacgg gctcgccatg ctcatcgaca
     3361 tcgaccgcaa cgtgtccgag ctgctcgggt ggcgggtcga ggacatggtg ggcgtgcgct
     3421 cgctggagtt catccacccc gacgaccagg acgagtccat cgcggcctgg atggacatgc
     3481 tcagcgcccc cgggctcacc acccggaccc ggctccgcca ctcccgcgcc gacggcagct
     3541 gggtgtggct cgacatctcc aaccgcaacc tgctcgagga ggagggccac gtcgagtgcg
     3601 agatggtcga cgtcagccag gaggtggcgg cccaggaggc cctcgccgcc agcgagcagg
     3661 tgctgcgccg cctgaccgag tcgatgccgg tgggcgtgtt ccacatcgac gccgacgagc
     3721 ggctccagct ggccaacgcc cgcctgcacg agatcctcgg caccaggccg ggtgacgacc
     3781 aggcgacgat gctcagcacg ctgcgcgacg agatggcctt cgaccgggcc ctctcggcgg
     3841 tgctggccgg caccgacgtc gacctcgagc tcgagatcca ggtgctcggc acgctcgatg
     3901 tccggcggtg cacgctcgcc ctccgggcct tgaccgtgga cggtgccgtc accggcgcgg
     3961 tcggcgtcct caccgacgtc accgagtccg ctcgcctccg cgaggagctg gagcgccggg
     4021 ccacgatgga cgacctcacc ggctgcttca accgagccag cgtgctcgag gcgctggagg
     4081 gcttcctcac caccggcacc gacgtggcgg tggtctacgt ggacatcgac gacttcaagg
     4141 tcgtcaacga cggccacggc cacgccgccg gcgacgaggt cctgcaggcg atcggcgccc
     4201 ggctgcgctc ggccgtccgg gagcacgacg ccgtgggccg catgggcggc gacgagttcc
     4261 tggccgtgtg cgccgggctc ggctccgagg acggtgcggc ggagctggcc cagcgcatcg
     4321 ccgcctcgct cagcgacccg gtcgggttcc gccgccagcg catcaccgtg tcggccagcg
     4381 tcggcgtggc gtggtcgcca ggcggcgagc agatcgacgc cgacgccctc gtcgcccggg
     4441 ccgacagcgc gatgtacgtc accaagggcg ccggcggcgg cgggcacacc gtccactgag
     4501 ccggagctac ggcgtgggca ggaaccgctt cgggtggcga ccgtccacgt agcccacgaa
     4561 gggcgggtag atgttgtacc cgagcagctc ctggagccgc tccggcagcg tggccaccac
     4621 ctcgggcgcc acgccgacga cgtggttctc ctgcgggcgt agccaggcgg cggcgtactc
     4681 gaggatgacg ccgaggcggg gctcgtcggt ggcgttggcc ccgccgccgt gccagatcga
     4741 gccggagtag aacgccacgc tgccggcggg catctcggcg tcgaggacgg ggtcgtcctc
     4801 ggtgggccag cgctccggtc cccaccggtg gctgccctcg acgaagcgag tggcgccgtt
     4861 ggcccgggtg aagtcgtcga aggcccacat cgtgttcacc accacctcgc cgtggggctg
     4921 gggcagcggg tagatgctgt cgtcggtgtg caggccctgg gccggttcgc cggggccgat
     4981 ctggatgccg gtcggggccg acagctggta gtggccgagg aggtcgtcga gcacgccgag
     5041 cagcacgggg tggatggcga ggtcgtcgaa ggagcgggtc ttgccgaaca ggttgtagat
     5101 ccgcttcgtc ccgtacccct cgaagtcgtt ccggccggtg ggggtggtct ccagcacgcg
     5161 gtcgaggtcg gcccggatgg cggcgatcgc gccggcatcg agcacgcgct cgcacacggc
     5221 cacgccgtcg gcgtggaccg cctcgaggat cgcctgggtg tcgaagtcga cggtgcggat
     5281 catgtcccct ccaggaaacg agcgttccca aactgctaca ccatgctccc atgggaacgt
     5341 ggaacttcgc tgacatctgg gagacggtcg ctgacgagct gcccgacgcc ccctgcctca
     5401 tccacggtga cgaccgccgg agctggtccg aggtcgaccg ccgcgccgac ggcgtggccc
     5461 agcacctgct ggatgccggg ctggagcgtc agcaggccgt ggcgctgtac ctctacaact
     5521 gccccgagta cctcgagggc acctacgccg cgttcaaggg cgccttcgtc ccggtgaaca
     5581 ccaactaccg gtacacggcc gacgagctgc tgtacctgtg ggacaacgcg gatgccggca
     5641 gcgtggtgtt ccacggcgcc ttcgtcgaca cgatcgagcc gatccgcgac cgcctgccga
     5701 aggtgaaggc ctggctgtgg gtcgacgacg gcagcggcgc ctgccccgac tgggccacgc
     5761 cgtacgagtc cgccgccacc agcggcgccg gccgggtctc gcccgagtgg ggccgcaccg
     5821 gcgacgacct gaacatgctc tacaccggcg gcaccaccgg catgcccaag ggcgtcatgt
     5881 ggcgccagga cgacctggcc gtgaagctca ccgccacgct cggcaacccg ctcaccgacg
     5941 acggcacggt cgaggacatc aagggcacct tcaacgagcc cggcgcccgc ttcctgcccg
     6001 cctgcccgca gatgcacggc accggcaact tcccctgcct gtccacgctg tccggcggcg
     6061 gctcggtggt caccctcacc aaccgcacct tcgacccggt cgagatgctc gacgccatcg
     6121 agcgcgaggg cgtgaacctg gtcgccctgg tgggcgacgc gttcggcaag ccgatcctgc
     6181 gcaccctcga cgagaacgag ggccggtggg acctctccag cctgctggcg atggtgtcct
     6241 cgggtgtcat gtggagcaag gagaccaagg agggcctgct ccgccaccac cccggcatga
     6301 tgctgatgga cgcgttctcg tcctccgagg ccctcggcat gggctcgtcc atctccggcg
     6361 ccggcgccac ggccgagacg gccaagttcg agctgagccc cgagaccatc gtcatcgacg
     6421 agagcaaccg accgatcgcg ccgggttccg acgagatcgg ccgcctggcc gtcggcgggc
     6481 gccagcccat cggctactac aaggacccgg agaagaccgc ggccacgttc ctcgagatcg
     6541 acggccagcg ctacagctgc ccgggcgact tcgccaccgt cgacgccgac ggcaagatct
     6601 cgctgctcgg ccgcggctcg gtgtgcatca acaccggcgg cgagaaggtg ttccccgagg
     6661 aggtcgagga ggcgctcaag acccaccccg ccgtcttcga cgccgtggcc gtcggcgtgc
     6721 cggacgagaa gttcggcgag gccgtcaccg ccgtggtcca ggtccgcgag ggcgccgacc
     6781 tcgacgaggg cgacgtcatc gcccacgtca agaccaagct cgccgcctac aaggccccca
     6841 agcgcgtcgt ggtggtcgac accatcggcc gggccgtgaa cggcaaggtc gactacaagc
     6901 gcctgaaggg cgaggcccag gagcgcttgg gcgtcagctg acgcccccgg tcccgggccg
     6961 cttcttcgcg gcggccttct tcgtcctcga gcgcgtcgcg gtggtcttct tcgccgcggc
     7021 cttcttccgg gcggccttct tcttggtggc tcgcttcttc gcagtggcct tcttcggggt
     7081 ggtcttcttc gccgtggcgc gtccgggcgt gcgggcctcg gcgagctgga cgggcacgtc
     7141 gacgtccatc agcttcgcca gcccctcgat gtcgtcgatg atgtcgtcga gcagcacgtc
     7201 gatgtcgggg accaggcggg cgcagccgac gaggccgaag tcgagcgtgt cgcagtagct
     7261 ctgcacggtg atgttgagcc cctggccctc cacgacggtg gacaccggca cgtagtgggc
     7321 cagcttcgcc ccggccatgt acagcggctc gcggggtccg gggacgttcg agatggtgat
     7381 gttcaccggc atggtcgtcc ggttgccgag ctggaaccgg gtggccatgc ggctggcccg
     7441 ggcgaacacg gcgggcggcg ggaagtcggc gaactcggtg agctggtcgg cgggcagcgc
     7501 gtcgtgcagg cccttggcca gcgccatggc gttgttcacc ttgcggatcc gctgcagcgg
     7561 gtcgggctcg tcggtgggca ggtcggcgaa gatcgccgag acccggttgg accagcgctc
     7621 ggtctcctcg ccggtgcgga tcgacaccgg gaccatggag accatcggct ggtcggggag
     7681 ggcatcgcgg cgctcgaggt aggtgcgcag gccgccggcg cacacggcca tgaccacgtc
     7741 gttcaccgtg cacccgatgg cgcccttgat cgccttgatg gactccatcg acaccgaccg
     7801 gtaggcgaag gagcggtgcg gggagatcga ggcgttgaag atggtcgacg gggcgttgtg
     7861 ggggaggggc tggggtgcct cgtcgcgctc ctccggacgt gaccgaccca ggttgagcac
     7921 cgtgccgagc ggcccgcgca ggccgtcgcg cagctgctcg cccgaggctc gcaggatcgg
     7981 gttgcggctg gcctcgccga gcgagcgcat cgtccgcgcg ccgaggacca ggaaccgtcc
     8041 cggcttgcgg gccagggtcc gggccgccat gccgaggacc tcggcgtcgg agggcaccgg
     8101 ctcgggctgg cggggcgcga gctgcgggct ggggatgtcg tcgccctcgg gcgtggtgtc
     8161 caacatgatc gtcagcagct cgacgccgga tgcgccgtcg acggtggcgt ggtggatctt
     8221 ggtgaggatg ccgaaccggt cgccggcgag cccgtcgatg acgtagctct cccacaacgg
     8281 gcgggaccgg tcgaggggcc gggcgatgat gcgggcgacc agcgccgcca cctggtcgtc
     8341 gcgcccgggt cgggggaccg cggtgtggcg gacgtggaag tcgaggtcga agtcgggatc
     8401 ctcgatccag aacgggtggt cgaggttgaa cggcacgttg cgcagccggc gggtgagcgg
     8461 ctcgagcagg tggagccggc gctggatctg ggcgcgccac tcctggagcg ggtcgaagtc
     8521 cggtgacggc cggtcgtaga tggagaggcc cgacacgtgc ccgtaggtcg acggtgtctc
     8581 catgtagagg aaggtggcgt cgagcccggt gagctgcttc atgtcgtgct cctggtgtca
     8641 ggttcgggtc atcgggccgg cggcccgtcc gtgggtggtc cggcgaaggc ctgggccttg
     8701 tgcatccagt cccgggcggc gtcgccggag acagtgaggt cggtgtcgtc gacgttgcgt
     8761 cgctgggtga cgacgaggca gaagtcgagg gcaggcccgg tgacggactc ggtggcgtcg
     8821 tcgggcccga aggtccacgt gtcgccgccg ggcgcgtcga gcaccacccg caccggcgtg
     8881 gccggcggct cctcgccgcg gttgatgtag gtccagcccc gggtgatgaa gccgaggcgg
     8941 gcgatgtggg cgaggcggtc cgtggcgggg cgctcggcgt cgaggccggc ggcgcgcagg
     9001 gcgtcgacca cgtcctggcc gtgggcccag cactccatga ggcgggcggt gaggaaggac
     9061 ttggcgccca tcgacggccc gtaccagggc acccggtcgt cgtcgctgag cccggtggcg
     9121 gcgtcggcga ggtcggcccg gttctcgcgc cagcgggcca gcaggtcctc gggggacgcg
     9181 gcgcgggccc agccgagggt gtggtcgtcc atggcctcct cgccgccctc gagcagctcg
     9241 accagctcgc cgagggaggc gttgaacgcg tccggatcgc ggatcgccag cgtcgccgtg
     9301 ccgtcgaagt aggtgaggtg ggcgatctgg tcggcgacgg tccagcgggg gctcggcgtc
     9361 ggcgtggccc actgctccgc ggtcaggtcg gcgaccaccg catcgagcgc gtcctgctcg
     9421 gcgatgaggt cggctcggat cccctgggcg tccacggcgg ccagcgttgc acgccggcga
     9481 ctgatcccgc tccgcgagtt gtgaggccct gccgagcaac tcgggtggcg agactcgccc
     9541 tgcgagtcga gactacgggc agcacccgtg gtccacgtcg acgcagtcga tgacgacgac
     9601 gtgggcgtcg ggtccgaagc ggcactcgag ggccaggcac tcgccgccgt cggggtggcg
     9661 cacccacgcc cagtggcagt gctcggtgtc gtcggtgcag tccgggcaat cgatcgcggt
     9721 ccgctcgctc acgacgcctc cgccatcgcc gagccggaca gggcggcgac cagctcgtcc
     9781 ttggtcatct tcgagcggcc gtcgatgtcg cggcgctggg cctcctcgta cagctcgtcc
     9841 ttggacatcg acgcgtagtc ggtgcccgac gcgctcgcgc tgctcgcacc ccgtggcgcc
     9901 tcgccgcgct tctggcgggc ctcgttgacg ctggcctcga gcacggccat gaggtcggtg
     9961 accgacgccg tgggctcctc ggccttctcg gtgacgatgc gctcgccgtc ggccttgcgg
    10021 gccaccaggt cgagcacccg ctcgcggtag gtgtcggtgt acttcgccgg ttcccagtcc
    10081 gtcgtgagcg actcgaccag gctcttggcc gccgcccgct cccggtcctt gatctcgatg
    10141 ccgtcgtcga gcacctgcat ctcgcggatg tcggcggggc tgcggacctc gtcgccgaag
    10201 tgcatggtct cgagcaccag cacgtcgccg gcgtcgccgt cgtcggagga cgcgacccgg
    10261 atggcggcca ggctctgctt ggcccgcatc acgaaccggg cgatggccac ccggttggag
    10321 tccttcatcg cctcgcggag cagggcgtag ggcttggccg cggcctggtc ggcgggggcg
    10381 aggtggtacg tcttgtcgaa gtagacgggg tcgacgtcgt cgaggtcgat gaagtcctcg
    10441 atgtcgatcg tgcgcgactt gcccggctcc acggcctcga gctcctcggg ggtgatgagc
    10501 acgtggctgc cgtcctcgag ctcgtagccc ttgacgatcg acccgtactc cacctccttg
    10561 ccggactgct cggtgacgcg ctggtagcgg atgcgctcac cgctctccgg ttcgaactgg
    10621 tggaagccga ccgagcggtc ctcggtggcg gtgaagagct tcacggggac gttcaccagg
    10681 ccgaagctga tgctccccgt ccagatggcg cgcggcatgc gcggtccttg cccacggcac
    10741 ggtgacgcca gacacgtggc cccggccgcg cggctacggt cccgaccgat gacggtcctg
    10801 cgctcccggc tcgacagcac ctccgacgag caccaggcga acgtggccgc catgcaggcc
    10861 ctgtgggacg aggtcgccga gcagctggcg tcggtgccgt ccgtcggcgg gcagcgctac
    10921 gtcgaccggc accggcgccg gggcaagatg ctcgtgcggg agcgcatcga ggcgctgatc
    10981 gacccgtaca cgccgttcct cgagctcagc ccgctggcgg cgtggggcac cgaggacccg
    11041 gtcggcgctg gcgtgtgcgc cggcatcggc atcgtgtgcg gcaccgaggt gatcgtcacc
    11101 gggtcggaca tgacctaccg cggcgggtcg gccaacccga cgacggtggc caagaacctg
    11161 cgcatcgacg agatctcccg tcgcaaccgg ctgccggtca tcacgctgaa cgagtccgcc
    11221 ggtgccgagc tgccccgcca ggccgacatc ttcgtgccgg gcggggccgg cttcaagaac
    11281 atcacccagc gctcgaagct gggcatcccg tcggtgacgg tcgtgttcgg tccctccact
    11341 gccggcggcg cgtacacgcc gggcatgagc gactacacgg tgttcgtcaa ggagcggggc
    11401 atggcctacc tgggcggtcc gccgctggtg aagatggcca tcgacgagga ggtcgacgag
    11461 gagaccctcg gcggcgccga gatgcactcg tcggtgtcgg gcctgtcgga ctacctcgcc
    11521 gccgacgaga tggacgccat ccgcatcgcc cgcgacatcg tcgcccacct gcgctgggag
    11581 aagctcggtc ccggtccctc cgagccggcc gacgagccgc tctacgaccc ggccgagctg
    11641 atcggtgccg cctcggccga cgtgcgggtg cccttcgacg tgcgcgaggt cctggcccgc
    11701 accctcgacg ggagccgctt cgaggagttc aagccccgct acggcgacaa gctggtctgc
    11761 gggtggggct cgatctgcgg gttcccggtg ggcgtgctgg ccaacaacgg gatcctgttc
    11821 tccgaggagt cgaacaaggg cgcccagttc atccagctgt gcaaccgcac cgacacgccg
    11881 ctggtgttcg tccagaacat caccggcttc atggtcggct cggccgccga gcggggcggc
    11941 atcatcaaga acggcgccaa gctcatcaac gcggtgtcga actccgaggt gccccacctg
    12001 gtcctgatgg tcggtgcgtc ctacggcgcc ggcaactacg gcatgtcggg ccgggcctac
    12061 gacccccggt tcgtgttctc ctggcccaac caccgcatcg ccgtcatggg acccaagcag
    12121 ctcgccggcg tgatggacat catcgcccgc aacgccgccg ccggccgggg ccaggaggtc
    12181 gacgaggagg ccctcgccgc acagacgtcc gcactcgaga acctggtcga ggagcagtcc
    12241 accgccctgt acgccatcgg ccggctgtgg gacgacggca tcatccaccc ggccgactcc
    12301 cgcaccgtgc tcggcatcgc cctgtcggcc acccactcgg ccgaggtgcg cggcgccacc
    12361 gactacggcg tgtggaggca ctagcgatga tcgttctggc ctacatgcat gaccaccaga
    12421 cggcacggat gtatgccaga acgggggtgg gggcgtgatc cggcggctgc tcatcgccaa
    12481 ccggggcgag atcgccgtgc gcatcgcccg caccgcccac cggctcggca tcgacaccgt
    12541 cggcgtgtac tcgacgtccg atgccgacgc cctccacgtc gacgtggtcg acctcgccgt
    12601 cgccctcggt ggcgccacgc cgaccgagtc gtacctgcgg gccgaggccg tcatccaggc
    12661 cgcgctcgac accggctgcg acgctgtcca ccccggctac ggcttcctgg ccgagcgggc
    12721 cgacgtcgcc cacatggtcg tcgacgccgg actgatctgg gtcgggccca cgccggagca
    12781 gatctcgctg ctcggcgaca agatcgccgc caagcgcgcc gcggtcgagg ccggcgtgcc
    12841 caccacggag gcggtcgagg tccggcccgg ccatgtcccc gacggtctga ccatgccggc
    12901 gctggtgaag gcggcggcgg gtggcggcgg gcgggggatg cgcatcgtgc ggtccgccga
    12961 ccagctgccc gatgccgtgg tcgcagcgtc ccgcgaggcc gaggccgcct tcggcgacgg
    13021 caccgtgttc gtggagccct tcatcgagcg gggccgccac gtcgaggtcc agatcgtcgg
    13081 cgacggccac ggcaacgtga tccacctcgg cgagcgcgag tgctcgatcc agcggcgcaa
    13141 ccagaaggtg ctggaggagt cgccctccgc cggcatcacg gccgagacgc gcaccgcgct
    13201 gtgcgacggt gccctcgccc tggcccgcca catcggctac cgcagcgccg gcaccgtcga
    13261 gttcatggtc ggcgacgacg ggaccgtcag cttcctcgag gtcaacaccc gcctccaggt
    13321 ggagcacccg gtcaccgagg ccgtcaccgg cctcgacctg gtcgagctgc agctccgggt
    13381 cgccgccggc gaggcgctgc ccctcaccca ggacgacgtc cgcttcgacg gtcacgccat
    13441 cgaggtccgc gtcgtcgccg aggaccccgc caccggctgg ctcccctcga ccggcaccgt
    13501 cacccggttc cagatcggga cggcagcggg ggcggacccc ttggaaaccg gcagtgcggg
    13561 tgtagcggca gtgcgggtcg acaccggatt ccgagccggc agcgtcgtgt ccgccgacta
    13621 cgactcgttg ctcgccaagg tcatctcgca cgccgccacc cggtccgagt cggccgccgt
    13681 gctcgcccgg gcgctgcgca gcgcggtggt caccggggtg cgtacgaaca tcgacgccat
    13741 ggctgcgctc ctggtcgagg acgacttcct cgaggcccgc acgcccaccg cctacctgga
    13801 cgagcacccc gacgtcgtcc acgccgccgg tcccatcggt gacgaccgcg tcgcccagct
    13861 cctc
//
LOCUS       DATISU010000049         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_235267, whole genome shotgun sequence.
ACCESSION   DATISU010000049 DATISU010000000
VERSION     DATISU010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235267"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1273
                     /gene="clpX"
                     /locus_tag="VLR27_02290"
     CDS             2..1273
                     /gene="clpX"
                     /locus_tag="VLR27_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HSP02299.1"
                     /translation="MAKFGDGGELLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEELAESSDLSFDELPRPREIFEFLEEYIVGQTDAKKILSVAVYNHYKRIQFGHHT
                     VHDDVEVAKSNILLLGPTGSGKTFLAQTLARLLKVPFAIADATALTEAGYVGEDVENI
                     LLKLIQAADYDVKKAETGIIYIDEIDKVARKSENPSITRDVSGEGVQQALLKILEGTQ
                     ASVPPQGGRKHPHQEFIQIDTSNVLFICGGAFAGIERLVESRLGRTGVGFAAEIRQGN
                     DRDVGELLNEVRPEDLVKFGLIPEFIGRLPVIGAVGHLDREALIEILTEPKNALLKQY
                     QKIFAYEDVELEFTQDALDAVADQALLRGTGARGLRAILEEVLLDIMYDIPGRTDVGK
                     VLVDEDVVRRRVNPTIVPRDTKADRPKRAAS"
     gene            1270..2604
                     /locus_tag="VLR27_02295"
     CDS             1270..2604
                     /locus_tag="VLR27_02295"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HSP02300.1"
                     /translation="MNFGDALVFLDEHINLEATLDGPKAGAVHGLSLDRMRGIVEVLG
                     DPQASAPVIHVTGTNGKGSTSRIITALLGAHGLTVGTYASPHLESITERIRRNVDSIS
                     EEDFGDIMGEIAALEPMFPDRPSYFDLLTAAAFAWFAREAVEVMVLEVGMLGRYDSTN
                     VASADVAVITNVGRDHTDFRGDWRRAIAEEKAGIIEDRSAVVIGETDPDLVEVFRAEG
                     GSMHWVRDEHFGVRSSLVALGGRHADLYTPGSRLEELYLPLHGAHQVDNASIAIAAVE
                     AFFGRPLDRDVVQEALRSLEIPGRFEVIDRAPLIVLDMAHNPDGAEAVADTYTSEFHP
                     EGGLTVVVGMLGGRDPDDTLAPLLALRPRRVICTRADSPRSVPAADIAAAVARGLGDH
                     VAEVRTIDDVDGAVRKAVDLLGDDEALLVTGSTYVVGAARATLRSLGFNARR"
     gene            2626..>2745
                     /locus_tag="VLR27_02300"
     CDS             2626..>2745
                     /locus_tag="VLR27_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HSP02301.1"
                     /translation="MNRTFVMCKPDAVERGLVGEIVARFERKGLTLVAAELKTA"
BASE COUNT          444 a         1015 c          890 g          396 t
ORIGIN      
        1 cgtggccaag ttcggagacg ggggcgagct cctcaagtgc agcttctgcg gcaagtccca
       61 gaagcaggtc aagaagctga tcgccggtcc cggcgtctac atctgcgacg agtgcatcga
      121 tctctgcaac gagatcatcg aggaggagct ggccgagtcc tcggacctca gcttcgacga
      181 gctgccccgt ccccgtgaga tcttcgagtt cctcgaggag tacatcgtcg gccagaccga
      241 cgcgaagaag atcctgtcgg tggcggtcta caaccactac aagcggatcc agttcggcca
      301 ccacaccgtc cacgacgacg tcgaggtcgc caagtccaac atcctcctgc tcggccccac
      361 cggctcgggc aagaccttcc tggcccagac gctggcccgc ctgctgaagg tccccttcgc
      421 catcgccgac gccaccgccc tcaccgaggc cggctacgtg ggtgaggacg tcgagaacat
      481 cctgctgaag ctgatccagg ccgcggacta cgacgtcaag aaggccgaga ccggcatcat
      541 ctacatcgac gagatcgaca aggtggcccg caagtcggag aacccgtcga tcacgcggga
      601 cgtgtccggc gagggcgtgc agcaggccct gctgaagatc ctcgagggca cccaggcgtc
      661 ggtgccgccc cagggcgggc gcaagcaccc gcaccaggag ttcatccaga tcgacacctc
      721 gaacgtcctg ttcatctgcg gcggggcctt cgccgggatc gagcgcctcg tcgagtcccg
      781 cctcggacgc acgggcgtcg ggttcgccgc cgagatccgc cagggcaacg accgagacgt
      841 cggggagctg ctgaacgagg tgcgccccga ggacctggtc aagttcggcc tgatccccga
      901 gttcatcggc cggctcccgg tgatcggcgc cgtcggccac ctcgaccgcg aggccctcat
      961 cgagatcctc accgagccca agaacgccct gctcaagcag taccagaaga tcttcgccta
     1021 cgaagacgtc gagctcgagt tcacccagga tgccctcgac gcggtcgccg accaggcgct
     1081 gctgcggggc accggcgcac gcggcctgcg ggcgatcctc gaggaggtgc tgctcgacat
     1141 catgtacgac atcccgggcc gcaccgatgt gggcaaggtg ctcgtcgacg aggacgtggt
     1201 gcgccggcgc gtcaacccca ccatcgtccc ccgcgacacc aaggccgacc ggcccaagcg
     1261 cgctgcctct tgaacttcgg tgacgcgctc gtcttcctcg acgagcacat caacctcgag
     1321 gccacgctgg acggccccaa ggccggtgcg gtgcacgggc tgtcgctcga ccgcatgcgg
     1381 ggcatcgtcg aggtgctggg cgacccccag gccagcgcgc cggtcatcca tgtcaccggc
     1441 accaacggca agggctcgac gtctcgcatc atcaccgcgc tgctcggggc ccacggcctc
     1501 accgtcggta cctacgcgag cccccacctg gagtcgatca ccgagcggat ccggcgcaac
     1561 gtcgactcga tctcggagga ggacttcggc gacatcatgg gcgagatcgc cgcgctcgag
     1621 cccatgttcc ccgaccggcc cagctacttc gacctgctga ccgccgccgc cttcgcctgg
     1681 ttcgcccggg aggccgtgga ggtcatggtg ctcgaggtgg ggatgctcgg ccggtacgac
     1741 tccaccaacg tggcgtccgc cgacgtggcg gtcatcacca acgtcggccg ggaccacacc
     1801 gacttccggg gtgattggcg acgcgccatc gccgaggaga aggccggcat catcgaggac
     1861 cgcagcgccg tggtcatcgg tgagaccgac ccggacctgg tcgaggtgtt ccgcgccgag
     1921 ggcggctcga tgcactgggt gcgcgacgag cacttcgggg tccgatcctc actggtcgcc
     1981 ctgggcggcc gccacgccga cctctacacg ccgggttccc gcctcgagga gctctacctg
     2041 ccgctgcacg gcgcccacca ggtcgacaac gcctcgatcg ccatcgccgc cgtggaggcc
     2101 ttcttcggcc gtccgctgga tcgtgacgtg gtccaggagg cgttgcggag cctggagatc
     2161 ccgggccgtt tcgaggtcat cgaccgcgcc ccgctcatcg tgctggacat ggcccacaac
     2221 cccgacggcg ccgaggccgt ggccgacacc tacaccagcg agttccaccc cgaaggtggc
     2281 ctcaccgtgg tggtgggcat gctcggcggc cgggaccccg acgacaccct cgcgccgctg
     2341 ctggcgctgc gaccccgccg ggtgatctgc acccgcgccg actcgccccg ctccgtgccg
     2401 gccgcagaca tcgccgcggc cgtcgcccgc ggcctcggcg accacgtcgc cgaggtccgc
     2461 accatcgacg acgtcgacgg ggcggtccgc aaggcggtcg acctgctcgg cgacgacgaa
     2521 gccctcctcg tcaccggatc cacctacgtg gtgggcgcgg cgcgggccac cctgcgcagc
     2581 ctcgggttca acgcacgccg ctgacggccg ctaccgtccc gccccgtgaa ccggaccttc
     2641 gtcatgtgca agcccgatgc cgtcgagcgc ggcctcgtcg gggagatcgt cgcccgcttc
     2701 gagcgcaagg ggctcaccct ggtcgccgcc gagctgaaga ccgcc
//
LOCUS       DATISU010000050         8988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_468186, whole genome shotgun sequence.
ACCESSION   DATISU010000050 DATISU010000000
VERSION     DATISU010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8988
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468186"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1885
                     /locus_tag="VLR27_02305"
     CDS             227..1885
                     /locus_tag="VLR27_02305"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HSP02302.1"
                     /translation="MGRGRQLIWAGAIAPVAAILLVVAAWAIDTGVAGGEVARNVELA
                     GADIGGRSASDLLEHVRALDAEMRDRPVRVVTPERTYETTAGEIGLSIDPRRTASLAL
                     DVGRSDPLPVRPVTWVASFFAPRDVEVRYEVDAETTRSAIRALEGDSLVAPQEPTIES
                     LDGEPFRAVPGRPGAGIDPDALAVALEAAAADAPVDEPVSVSIQPADLAPMLDDSAAE
                     AAAVTANEMTAETISITAADRTIELVPRELRQMAQVSQVDDGITITLPVDGVLAVLEA
                     EFGDLAVEPRDASFSVSGGQVQLQPGVTGRSCCSEESAQTVVDAFAAGITSVEIELET
                     TEPSLTTAEAEAYGIVEEIGSPNEFGPTTRHACCQNRVTNIHRIADIIRGVVIPPGGS
                     FSVNDYVGRRTIEKGFVSDGVIYDGVLTEDVGGGISQFATTFFNAALFAGLEFGEYQS
                     HSLYISRYPRGHEATISYPAPDLVIENPSPYGVLVWPEYTDTSITVRLFSTTWAQVSV
                     GEPSPSDQGNCTRWTTPRTRTFPDGSTDTDTVAAVYRPEEGVSC"
     gene            1981..3351
                     /locus_tag="VLR27_02310"
     CDS             1981..3351
                     /locus_tag="VLR27_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HSP02303.1"
                     /translation="MSDSDALMWNIEKDPILRSTITAVAVLDRRPDREALLRRIERGT
                     RLIPRMRQRVIGNPYSIAPPRWEVDPHFDLRYHVRFVHAGGDGSLRDVLDIAEPIGMQ
                     GFDRARPLWEFVIVEGLEGDRAAIIQKIHHAITDGVGGMQMVMTMIDLEREPAATDEE
                     MPPAPEAEAMGPLQRTADAFGHVRRRNLGIARRAGGNLVAAATTAASHPASVVAGLGA
                     TAGSVGRLLAPTPTPLSPIMTGRSMSFRFQTLTFPLPDLKAAAKAAGGTLNDAFVVAV
                     AGGLRRYHQRHGVDVEALRMTMPINIRTEDTSDVAGNQFVPARFTVPVAIEDPTEHMR
                     AIHELVDQQRHEPANALVEPLAIVLNRLPTTAVTALFGSMIKGSDFTTSNVPGAPIPI
                     YIAGARVESMIPFGPLVGVAVNVTLLSNLDDVHVGINSDMAAVPDPEVLLECLQESFD
                     DVLKQA"
     gene            3379..4599
                     /locus_tag="VLR27_02315"
     CDS             3379..4599
                     /locus_tag="VLR27_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HSP02304.1"
                     /translation="MLTCDVVVVGAGPAGSSAATSLARAGRDVLLVDKATFPRDKCCG
                     DGLTALALRLTERLGLDPTSLPSWTTVDSAVVSGPKGHAVRFPLPEGPGTHAAVVRRS
                     ELDAALVDLARQAGAKVLDGHAMVGIAEHDDRVVLDVAGLGEVHARYAVAADGMWSPT
                     RRALGLSVPGYRGDWHAFRAYFRNVSETALRDLCVWFEADLLPGYAWSFPVGDDTANF
                     GFGIQRGGKVAVGDMKQIWDDLLARPAIREVLGPDAVPEARFTAWPIPARVHDMSRTG
                     RRTFFVGDAVAATDVMTGEGIGQAILTGMLAAEAIEETGPHGARVATERYAAAVDHHL
                     VADHHVSALLVRALRHRKGARAAIRVAGLTPWTRRNFARWLFEDYPRAIVGTPSRWHR
                     GVFRGPGAYAEPSA"
     gene            4632..5600
                     /locus_tag="VLR27_02320"
     CDS             4632..5600
                     /locus_tag="VLR27_02320"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HSP02305.1"
                     /translation="MRTRLTDILEIEHPVMLAGMGGVSYADLVVAVSEAGGFGCLGAS
                     TMSDDELDAEMADVRSRTDKPYGVDLLTAAGDQTAKVERIIAAGATLYVAGLGVPREV
                     VDLCHAKGVLVMNMCGKVRHAIDAVEAGCDLVVAQGTEAGGHTGRIATNTLVPLIVDA
                     VGDRVPVVAAGGIFDGRGLASALTLGADGIWVGTRFIATPEAHTVDGYKEKLVSMAED
                     DTIVTRAYTGKTCRVVRTSYADEFEAAGGQPEPFPMQVFKSMEDGANHLGAEGSPEGL
                     DIDREFMPAGQGAGGIQDLVPAAELVERFVAEAEQALARAQGAVTA"
     gene            5643..6908
                     /locus_tag="VLR27_02325"
     CDS             5643..6908
                     /locus_tag="VLR27_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02306.1"
                     /translation="MPALVETALVRRHDRRTGDWAEVRTDGRRITVASGGTGNRLDPE
                     RRVRRSGWEIRRIGDQLELLSTWPVHELVTHRIGEHVAQLADDVARAGGPPDGDLAAV
                     ADAVPDLLDEDDPGDPPPLDALLWSRAYPLLRVPIERGAHLDAVPVALDQVLRAADPR
                     RAARRAFGRVTRPLVRALAASLLPAPDGRVPFEPALLALMASPWCGPEQLTEILGRRP
                     HRAGAVAFDVGEVDRARAMFDGVASRRIAARLGAALVEPDGLRALATELATWVPPPGE
                     PRTGAAHPPGARAPAAAVTPPARTDVPLRHPPALQGVDDLEVGRRRLVLPRTADELVE
                     WGAVLDNCLGGFRHSVAAGRTHVVGVVVGGRLRWAVEITRAGVVRQFEGAGNRQAPEH
                     VVQPVLAALRSRGVVRADGRRGYSLMAPP"
     gene            complement(6922..7386)
                     /locus_tag="VLR27_02330"
     CDS             complement(6922..7386)
                     /locus_tag="VLR27_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02307.1"
                     /translation="MTLVEPEVAPVAATPDVDPALIAAEIGNDGDLKRKSKVRAFTPL
                     PGVPEGTPGKVSLVNGWDLWIRYHVMFDNGVNLGSINREHLVPAKQYDDMVEKRRQAL
                     ESGVFDEPEVDEAEAEGGGGEALGGEAPVVNGVSIPPHLIERSAAARKRLGA"
     gene            complement(7489..>8988)
                     /locus_tag="VLR27_02335"
     CDS             complement(7489..>8988)
                     /locus_tag="VLR27_02335"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HSP02308.1"
                     /translation="QVVTGLPSIGQDGEAVGPADVAFSPDGVMHVIIGLGGNLEFRDH
                     FTDARIGTVITVDEEGGTEVLADLVQFEHDNDPDAAYNDGLPEDAMPISEGVDSNPFG
                     LTFDGDDVLAVDAGGNTVLRIAPDGTTSVEHLLPVGMSEAPPFLGLPPGTMIPHQPVP
                     TAIDVDAEGTPLVGELTGFPFPVGGADVYDVSGEEPESVIAGLTNILDIDVDVETGNV
                     YVLEFADNGLMAEAPAPALVEVRPDGSMKYLLYGDELPPPGGVEVGPDGMIYLTACTL
                     CGPDAGMVWQLDPSVPADPATAEACDPAVVPGAGFPDTKASVHREAIDCLAFWGAVEG
                     YADGTFGPRTAIRRDQVASMLARALDAAGVELPAAPPDAFTDDDDNVHEPSIDALADI
                     GVVEGYPDGTYRGSEHVTRAQIASMAARAWEAVTGEALADGADAFTDDDDSVHEDNID
                     AVAAAGWVNGVSEDLFAPQHPAVRAQFASILARMLSSLVDGGHAAMPAA"
BASE COUNT         1189 a         3439 c         3139 g         1221 t
ORIGIN      
        1 agggagtgct cgcggccgat cccccgtccg gctccggtca cgatcgcgac ccgtccgtca
       61 cagatgccac tcatgtgtgt gcgtcccttc tcgtctcctc gccggttctc ggtcgagggc
      121 cgcgtgacca taggcacgcg acgtgggctc cgtcatgtac gtcaacggat cgtcatcgct
      181 ctcgactacg ccggcccctg ggtggcgtgc cagggttgct cgtcgtatgg gtcgtgggcg
      241 acagctgatc tgggcaggcg cgatcgcacc cgtcgcagcg atcctcctcg tcgtcgcggc
      301 gtgggcgatc gacaccggcg tcgccggagg cgaggtcgcc cgcaacgtgg agctggccgg
      361 cgccgacatc ggcggccgca gcgccagcga cctgctcgag cacgtgcggg cgctcgacgc
      421 cgagatgcgg gaccgacccg tccgcgtcgt cacgccggag cggacctacg agaccaccgc
      481 cggcgagatc ggcctctcca tcgacccgcg ccgcacggcc agcctcgccc tcgacgtcgg
      541 ccgcagcgac cctttgcccg tgcgtcccgt cacctgggtc gccagcttct tcgcgccgcg
      601 cgacgtcgag gtccgctacg aggtcgacgc cgagaccacc cgctcggcca tccgggcgct
      661 cgagggcgac tccctggtgg caccccagga gcccaccatc gaatccctcg acggcgagcc
      721 gttccgtgcc gtgcccggtc gtcccggcgc cggcatcgat cccgacgccc tcgccgtggc
      781 gctcgaggcc gccgccgccg atgcgccggt ggacgagccg gtgtcggtgt cgatccagcc
      841 cgccgacctc gcaccgatgc tcgacgacag cgccgcggag gccgcggcag tgacggccaa
      901 cgagatgacg gccgagacga tctcgatcac cgccgcagac cgcacgatcg agctggttcc
      961 ccgcgagctg cgccagatgg cgcaggtctc ccaggtcgac gacggcatca ccatcaccct
     1021 ccccgtcgac ggggtcctcg ccgtgctcga ggccgagttc ggggacctcg ccgtcgagcc
     1081 ccgcgatgcc agcttctcgg tgtcgggtgg ccaggtccag ctgcaaccgg gggtcaccgg
     1141 gcgctcgtgc tgctcggagg agtccgccca gaccgtggtc gatgccttcg ccgccgggat
     1201 cacctcggtg gagatcgagc tggagacgac cgagccatcg ctcaccaccg ccgaggccga
     1261 ggcctacggc atcgtcgagg agatcggcag tccgaacgag ttcggcccca ccacgcgcca
     1321 cgcgtgctgc cagaaccggg tgacgaacat ccaccgcatc gccgacatca tccgcggcgt
     1381 cgtcatcccg cccggcggct cgttctcggt caacgactac gtcggtcgcc gcaccatcga
     1441 gaagggcttc gtgtccgacg gggtcatcta cgacggcgtg ctcaccgagg acgtcggcgg
     1501 cgggatcagc cagttcgcca ccacgttctt caacgccgcc ctgttcgccg ggctcgagtt
     1561 cggcgagtac cagagccaca gcctctacat ctcccgctac ccccgggggc acgaggccac
     1621 catctcgtac cccgccccgg acctggtgat cgagaacccg agcccgtacg gcgtgctcgt
     1681 gtggcccgag tacaccgaca cctcgatcac cgtgcggctc ttcagcacga cctgggccca
     1741 ggtgtccgtc ggcgagccct cgcccagcga ccagggcaac tgcacccggt ggaccacgcc
     1801 ccggacccgc accttccccg acggctccac cgacaccgac acggtcgccg ccgtttaccg
     1861 tcccgaggag ggcgtgagct gctgacccgg ctcgcgaaca gggacatctg accctggtgg
     1921 cgaacgttgc ccccggtgca ccctcggacc ccgggagcga cgatgcagta cgagacgcgg
     1981 atgagcgaca gcgatgcgct catgtggaac atcgagaagg atccgatcct gcgatccacc
     2041 atcaccgcgg tcgcggtgct ggaccgccgg ccggaccggg aggccctgct ccgccggatc
     2101 gagcgcggca cccggctcat cccgcgcatg cgccagcggg tgatcggcaa cccgtactcc
     2161 atcgccccgc cccgctggga ggtcgacccg cacttcgacc tgcgctacca cgtccggttc
     2221 gtgcacgccg gcggcgacgg gagcctgcga gacgtgctcg acatcgccga gccgatcggg
     2281 atgcagggct tcgaccgggc ccggcccctg tgggagttcg tcatcgtcga gggcctcgag
     2341 ggcgaccggg ccgccatcat ccagaagatc caccacgcca tcaccgacgg ggtcggcggc
     2401 atgcagatgg tgatgacgat gatcgacctc gagcgcgagc cggcggccac cgacgaggag
     2461 atgccaccgg cgcccgaggc cgaggccatg gggccgctgc agcggaccgc cgacgcgttc
     2521 ggccacgtgc ggcgccgcaa cctgggcatc gcccggcggg ccggcggcaa cctcgtcgcc
     2581 gccgccacga ccgccgcgtc ccaccccgcc tcggtcgtcg ccggcctcgg cgccaccgcc
     2641 gggtcggtgg gccgtctgct cgccccgacc ccgaccccgc tcagcccgat catgaccggg
     2701 cggtcgatgt cgttccggtt ccagacgttg acgttccccc tgcccgacct caaggccgca
     2761 gccaaggccg ccggcgggac cctgaacgac gcgttcgtcg tggccgtcgc cggcgggctg
     2821 cgtcgctacc accagcgtca cggcgtggac gtcgaagccc tgcggatgac gatgcccatc
     2881 aacatccgca ccgaggacac cagcgacgtg gcgggcaacc agttcgtccc ggcccgcttc
     2941 accgtcccgg tcgcgatcga ggacccgacg gagcacatgc gggcgatcca cgagctggtg
     3001 gaccagcagc gacacgagcc ggccaacgcg ctggtcgagc ccctcgccat cgtcctgaac
     3061 cgcttgccga ccaccgccgt caccgcgctg ttcggctcga tgatcaaggg ctcggacttc
     3121 accacctcga acgtgcccgg cgccccgatc ccgatctaca tcgccggcgc ccgggtggag
     3181 tccatgatcc ccttcggtcc cctggtcggg gttgcggtca acgtgaccct gctgtcgaac
     3241 ctcgacgacg tccacgtcgg gatcaactcg gacatggcgg cggtgcccga tcccgaggtg
     3301 ctgctcgagt gcctgcagga gtccttcgac gacgtcctga agcaggcgtg agcgcacgcg
     3361 ctcaatagat tcgaccccgt gctgacctgt gacgtcgtcg tcgtcggcgc aggaccggcc
     3421 gggtccagcg cggccacgag cctggctcgg gccggccggg acgtcctcct cgtcgacaag
     3481 gccaccttcc cgcgcgacaa gtgctgcggc gacgggctca ccgcgctggc cctgcgcctc
     3541 accgagcgcc tcggcctcga ccccacgtcg ctcccgtcct ggacgacggt ggacagcgcc
     3601 gtggtgtcag gcccgaaggg gcacgccgtg cggttccccc tgccggaggg cccgggcacg
     3661 catgccgcgg tggtgcggcg cagcgagctc gacgccgcgc tcgtcgacct cgcccggcag
     3721 gccggcgcca aggtgctcga cggccacgcc atggtcggca tcgccgagca cgacgaccgg
     3781 gtcgtcctcg acgtggccgg cctcggagag gtccatgccc gctacgcggt ggccgccgac
     3841 gggatgtggt cgcccacccg gcgggccctc gggctgagcg tgccgggcta ccgcggcgac
     3901 tggcacgcct tccgcgccta cttccgcaac gtgtccgaga ccgccctgcg cgacctgtgc
     3961 gtgtggttcg aggccgacct gctccccggc tacgcctggt cgttccccgt cggcgacgac
     4021 accgccaact tcggcttcgg catccagcgg ggcggcaagg tcgcggtcgg cgacatgaag
     4081 cagatctggg acgacctgct cgcccggccc gccatccgcg aggtcctcgg ccccgacgcg
     4141 gtgcccgagg cccggttcac cgcgtggccc atcccggccc gggtgcacga catgtcccgc
     4201 accgggcggc gcacgttctt cgtcggcgac gccgtggccg ccaccgacgt gatgaccggg
     4261 gagggcatcg gccaggcgat cctcaccggg atgctggccg ccgaggccat cgaggagacc
     4321 gggccccacg gcgcccgggt cgccaccgag cgctacgcgg ccgccgtcga ccaccacctg
     4381 gtggccgacc accacgtcag cgccctgctg gtgcgggccc tgcgccaccg caagggcgcc
     4441 cgggcggcca tccgggtcgc cgggctcacc ccgtggaccc ggcgcaactt cgcccggtgg
     4501 ctgttcgagg actacccccg cgccatcgtc ggcacgccca gccgctggca ccggggcgtc
     4561 ttccgcggcc cgggcgccta cgccgagccc tctgcttgac cgctcggtca acgcctggcc
     4621 acgatgccgg gatgcgcact cgtctgaccg acatcctcga gatcgagcat ccggtgatgc
     4681 tcgccggcat gggcggggtc tcctacgccg acctcgtcgt ggccgtgtcc gaggccggcg
     4741 ggttcgggtg cctcggcgcc tcgaccatgt ccgacgacga gctcgacgcc gagatggccg
     4801 acgtgcggtc ccgcaccgac aagccctacg gcgtcgacct gctcaccgcc gccggggacc
     4861 agacggccaa ggtcgagcgg atcatcgccg ccggcgccac cctgtacgtg gccggcctgg
     4921 gcgtgccccg ggaggtggtc gacctgtgcc acgccaaggg cgtgctcgtc atgaacatgt
     4981 gcggcaaggt gcgccacgcc atcgacgccg tcgaggccgg ctgcgacctc gtggtcgccc
     5041 agggcaccga ggccggcggg cacacgggcc ggatcgccac caacaccctc gtccccctga
     5101 tcgtcgacgc cgtcggcgac cgcgtaccgg tcgtggcggc cggcggcatc ttcgacgggc
     5161 gcggcctggc gtcggccctg acgctcggcg ccgacggcat ctgggtgggc acccgcttca
     5221 tcgccacgcc cgaggcccac acggtcgacg gctacaagga gaagctggtc tcgatggccg
     5281 aggacgacac catcgtcacg cgcgcctaca ccggcaagac ctgccgggtg gtccgcacca
     5341 gctacgccga cgagttcgag gccgctggcg gccagcccga gcccttcccc atgcaggtct
     5401 tcaagtcgat ggaggacggg gccaaccacc tcggcgccga gggctccccc gagggcctgg
     5461 acatcgaccg cgagttcatg ccagcgggcc agggtgcggg cggcatccag gacctggtgc
     5521 cggccgccga gctggtcgag cgcttcgtgg ccgaggccga gcaggcgctg gcccgggccc
     5581 agggcgccgt caccgcgtag tcgacgtcgc cccgaggtca cgtccgcccc gtaccgatcc
     5641 tggtgcccgc cctcgtcgag accgccctcg tccgcagaca cgaccggcgc accggcgact
     5701 gggccgaggt tcgcacggac gggcgccgca tcaccgtggc gtccgggggc accgggaacc
     5761 gcctcgaccc ggagcgccgg gtccgccgga gcggctggga gatccgccgc atcggggacc
     5821 agctcgagct gctgtccacc tggccggtgc acgagctggt cacgcaccgc atcggtgagc
     5881 acgtcgccca gctcgccgac gacgtcgccc gggccggcgg cccacccgac ggggacctgg
     5941 ctgccgtcgc cgacgccgtc cccgacctgc tcgacgagga cgacccgggc gaccccccgc
     6001 cgctcgatgc gctcctgtgg tcccgggcct acccgctgct ccgggtcccg atcgagcggg
     6061 gcgcccacct cgacgccgtg cccgtggccc tcgaccaggt gctccgggcc gccgaccccc
     6121 gccgcgcggc ccgccgcgcc ttcggccggg tcacccggcc cctcgtgcgg gccctcgccg
     6181 ccagcctcct acccgccccg gatggtcggg tgccgttcga gccggcgctg ctcgccctca
     6241 tggccagccc gtggtgcggg ccggagcagc tgaccgagat cctcggtcgc cggccgcacc
     6301 gtgccggtgc ggtggccttc gacgtcggcg aggtcgaccg ggcccgagcg atgttcgacg
     6361 gggtggcgtc ccgccggatc gctgcccggt tgggcgcggc cctggtcgag cccgacgggc
     6421 tccgagccct cgccaccgag ctcgccacct gggtgccacc ccccggcgag ccccggaccg
     6481 gggcagcgca cccgccgggg gctcgggcgc ccgcagcggc cgtcaccccg cctgcccgca
     6541 ccgacgtccc cctccgccac ccaccggcgc tccagggcgt cgacgacctg gaggtcgggc
     6601 gccgccggct ggtgctgccc cgcaccgccg acgagctggt cgagtggggc gccgtgctgg
     6661 acaactgcct cggcgggttc cgccactccg tcgccgccgg gcgcacccac gtggtcggcg
     6721 tcgtcgtcgg cggacggctg cgctgggcgg tggagatcac gcgggccggc gtggtccgcc
     6781 agttcgaggg cgccggcaac cgccaggccc ccgagcacgt ggtccagccg gtgctcgccg
     6841 ccctgcggtc ccgcggcgtg gtgcgcgccg acgggcggcg cgggtactcc ctgatggcac
     6901 ccccctgacg gggtggggcg gctacgcgcc gaggcgcttg cgggccgcgg ccgagcgctc
     6961 gatgaggtgc ggcgggatgg agacgccgtt cacaaccggg gcttcgccac cgagcgcctc
     7021 gccgccgcca ccctcggcct cggcctcgtc gacctcgggc tcgtcgaaga caccggactc
     7081 gagtgcctgg cggcgcttct cgaccatgtc gtcgtactgc ttggccggca ccaggtgctc
     7141 gcggttgatc gagccgaggt tcacgccgtt gtcgaacatg acgtggtagc ggatccacag
     7201 gtcccacccg ttgaccaggg agaccttgcc cggggtgccc tcggggacgc cgggcagcgg
     7261 cgtgaaggcc cgcaccttgg acttgcgctt cagatcgccg tcgttgccga tctcggcggc
     7321 gatcagggcc ggatcgacgt cgggggttgc agccacgggg gccacctcgg gctcgaccag
     7381 ggtcatgcgc ccaatgctgg cacggcggcc gccaccgcac ggaactctcc cccgatccgg
     7441 gacggccgga cccgggagct cgcgctcccg ggtccggcca cctcccggtc aggcggctgg
     7501 catcgcagcg tgaccgccgt cgaccagcga cgacagcatc cgggcgagga tgctggcgaa
     7561 ctgggcccgc actgcggggt gctgcggggc gaagaggtcc tcgctcaccc cgttcaccca
     7621 cccggctgcg gcgacggcgt cgatgttgtc ctcgtggacg ctgtcgtcgt cgtcggtgaa
     7681 cgcgtcggct ccgtcggcca gcgcctcgcc cgtgaccgcc tcccacgccc gggccgccat
     7741 cgaggcgatc tgggcccggg tgacgtgctc ggacccgcgg taggtgccgt cggggtagcc
     7801 ctcgaccacg ccgatgtccg ccagcgcatc gatgctgggc tcgtggacgt tgtcgtcgtc
     7861 atcggtgaag gcgtccggcg gtgctgccgg gagctccacg ccggcggcgt ccagcgcccg
     7921 ggccagcatc gaggccacct ggtcgcggcg gatggccgtc cgcggcccga aggtgccgtc
     7981 ggcgtagccc tcgaccgcgc cccagaaggc caggcagtcg atggcctcgc ggtggaccga
     8041 ggccttggtg tcagggaacc cggcaccggg cacgacggca gggtcgcagg cctcggcggt
     8101 ggcgggatcg gccggcaccg acggatcgag ctgccacacc atgccggcgt caggaccgca
     8161 caacgtgcag gccgtcaggt agatcatccc gtcggggccg acctcgacgc cgcccggagg
     8221 cggcagctcg tcgccgtaga ggaggtactt catgctcccg tcggggcgga cctccaccag
     8281 ggcaggggcc ggtgcctcgg ccatcagccc gttgtcggcg aactccagga cgtagacgtt
     8341 gcccgtctcg acatccacgt cgatgtcgag gatgttggtg aggcccgcga tgacggactc
     8401 gggttcctcg ccggagacgt cgtagacgtc ggcgccgccc acggggaacg ggaacccggt
     8461 cagctcgccc accaggggcg tgccctcggc gtcgacgtcg atggccgtcg gcacgggctg
     8521 gtgcgggatc atcgtgccgg gcggcaggcc gaggaacggc ggcgcctcgg acatgccgac
     8581 aggcaggagg tgctcgacgg aggtggtgcc gtcgggcgcg atccgcagga cggtgttgcc
     8641 gcccgcgtcg accgccagga cgtcgtcgcc gtcgaaggtc aagccgaacg ggttcgagtc
     8701 cacgccctcg ctgatcggca tggcatcttc cggcagaccg tcgttgtagg ccgcgtcggg
     8761 gtcgttgtcg tgctcgaact ggacgaggtc ggcgaggacc tcggtcccgc cctcctcgtc
     8821 gaccgtgatc accgtgccga tgcgggcgtc ggtgaagtgg tcgcggaact cgaggttccc
     8881 gcccaggccg atgatcacgt gcatcacgcc gtcgggcgag aacgccacgt ccgccggccc
     8941 gacggcttcg ccgtcctggc cgatcgaggg caggccggtg acgacctg
//
LOCUS       DATISU010000051         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_794115, whole genome shotgun sequence.
ACCESSION   DATISU010000051 DATISU010000000
VERSION     DATISU010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794115"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VLR27_02340"
     CDS             <1..547
                     /locus_tag="VLR27_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HSP02309.1"
                     /translation="GTTETALISLGGIVALQAVRVGSFDIDAAIQSYVRREYGIAIGE
                     RTAEEIKIAIGSAWPIDDEVRAEVRGRDLMTGLPKTVILSKPEVREAISEPVTAIVDS
                     VIACLGQAPPELADDLISTGMQLVGGGGMLQGLDRRISVETDIPVQLVQQPLESVVLG
                     AGRCVEAYDDLKVMFMGGRAV"
     gene            835..1542
                     /locus_tag="VLR27_02345"
     CDS             835..1542
                     /locus_tag="VLR27_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP02310.1"
                     /translation="MDGLSFAIDEGQICALIGPNGAGKTTLFNVVSRVYDPTEGKITY
                     DGKDLIHLAPHTIARVGIARTFQNLALWPGMSVLENVMVGMHTTSKQSFLTAALRLGL
                     SKEERELRDKAYAVLEELDLQAFARHPAAGLPFGTLKRVELARAIVSDPRLLLLDEPA
                     GGLTHSEVDELASVIKKVRDDRNLTVLLVEHHMHMVMGISDKVVVLDFGSKIAEGLPS
                     EIQRDERVIEAYLGAPA"
     gene            1539..>2004
                     /locus_tag="VLR27_02350"
     CDS             1539..>2004
                     /locus_tag="VLR27_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSP02311.1"
                     /translation="MTDATTTSADVPASTGKPILQLADVHAAYGMVRVLHGIDLEVRD
                     GEVVVVLGANGAGKTTTMRAICGLVDTTGSILLDGKEIVGKKSADVARLGVAHVPQGR
                     GTFADLSVEDNLQVGAFVRKDKEIRSDIDRWYDVFPVLGERRQQQAGTLSGGE"
BASE COUNT          324 a          731 c          664 g          285 t
ORIGIN      
        1 tggcaccacg gagaccgcgc tgatcagcct cggcggcatc gtcgccctgc aggccgtgcg
       61 cgtcggctcc ttcgacatcg acgccgccat ccagtcctac gtgcggcgcg agtacggcat
      121 cgccatcggc gagcgcacgg cagaggagat caagatcgcc atcgggtcgg cgtggccgat
      181 cgacgacgag gtgcgcgccg aggtccgggg acgcgacctc atgaccggcc tgccgaagac
      241 ggtgatcctc tccaagcccg aggtgcgcga ggccatcagc gaaccggtca ccgccatcgt
      301 cgactccgtc atcgcctgcc tcggccaggc accccccgag ctggccgacg acctcatcag
      361 caccgggatg cagctcgtcg gcggtggcgg catgctccag ggcctcgacc gccggatctc
      421 ggtcgagacc gacatcccgg tccagctcgt ccagcagccg ctcgagtcgg tcgtgctcgg
      481 cgcggggcgc tgcgtggagg cctacgacga cctcaaggtg atgttcatgg gcggccgcgc
      541 cgtctgagca gctccctgcc cgggcctgtc atcgcatcgt catcgttccg tcgggggacg
      601 tagcaggtcg aaccggcacg atccggacga agaaggtgtg acatgcgtcg acggcgggtg
      661 ccgagcggtg taggttgcgc ccgctaatga cggagcgtcc agtaggtccc gcaggggacg
      721 gggacactcc gactgcatga ctgcccgccg ccacgggccg ttccaggggg aaatcacatc
      781 gctgcactac tcgacgtctc cgacgtcacc gtccgcttcg gcggcgtcgt cgccctggat
      841 gggctgtcgt tcgccatcga cgaaggccag atctgcgcgc tgatcggccc gaacggcgcg
      901 ggcaagacca cgctgttcaa cgtcgtcagc agggtgtacg accccaccga ggggaagatc
      961 acctacgacg gcaaggacct catccacctc gcgccgcaca cgatcgcccg ggtcggcatc
     1021 gcccgcacgt tccagaacct ggcgctgtgg cccggcatga gcgtgctcga gaacgtcatg
     1081 gtcggcatgc acacgacgtc gaagcagtcc ttcctcaccg ccgccctgcg cctcgggctc
     1141 agcaaggagg agcgcgagct gcgggacaag gcctacgccg tcctcgagga gctcgacctg
     1201 caggccttcg cccgccaccc cgccgccggg ctcccgttcg gcaccctcaa gcgggtggag
     1261 ctggcccggg ccatcgtgtc cgacccccgg ctcctgctcc tcgacgagcc cgccggtggc
     1321 ctcacccaca gcgaggtcga cgagctcgcc tcggtcatca agaaggtccg ggacgaccgc
     1381 aacctcacgg tgctgctggt cgagcaccac atgcacatgg tgatgggcat ctccgacaag
     1441 gtcgtcgtgc tcgacttcgg ctccaagatc gccgaaggcc tgccgtcgga gatccagcgc
     1501 gacgagcggg tcatcgaggc ctacctgggg gcaccggcat gaccgacgcg accaccacct
     1561 ccgccgacgt gccggcctcc accggcaagc cgatcctgca gctcgccgac gtgcacgccg
     1621 cctacggcat ggtgcgggtg ctgcacggca tcgacctcga ggtgcgcgac ggcgaggtcg
     1681 tcgtggtgct cggggccaac ggcgccggca agaccaccac gatgcgggcc atctgcggcc
     1741 tggtcgacac caccgggtcg atcctgctcg acggcaagga gatcgtcggc aagaagtccg
     1801 ccgacgtcgc ccgcctcggc gtggcccacg tcccccaggg acgaggcacc ttcgccgacc
     1861 tgtccgtcga ggacaacctg caggtcggcg ccttcgtccg caaggacaag gagatccgct
     1921 ccgacatcga ccgctggtac gacgtcttcc ccgtcctcgg ggagcgccgc cagcagcagg
     1981 ccggcacgct ctcgggcggc gagc
//
LOCUS       DATISU010000052         6438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_608060, whole genome shotgun sequence.
ACCESSION   DATISU010000052 DATISU010000000
VERSION     DATISU010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608060"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VLR27_02355"
     CDS             complement(<1..253)
                     /locus_tag="VLR27_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSP02312.1"
                     /translation="MARFAVIGAGIAGLSAAWELERAGHDVVVFESAREIGGKLQRSR
                     VRGLAFPLEEGADAFLARVPDALELCAEIGIDELVHPASS"
     gene            complement(253..1302)
                     /gene="hemE"
                     /locus_tag="VLR27_02360"
     CDS             complement(253..1302)
                     /gene="hemE"
                     /locus_tag="VLR27_02360"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HSP02313.1"
                     /translation="MAPRPDHPFLQACRGLPAEHVPVWFMRQAGRSLPEYNQVKGDVS
                     ILDAVERPELAAELTMQPVHRYGVDAAIFYSDIVVPARAIGFGIDIVAGVGPVAEQPF
                     RTKEDLERLPVLVPEEHSPYIAETVRILVDELGDRPLIGFAGAPFTVASYLIEGRPSR
                     TYEHTKRMMFAEPELFAALLDRLADIAIASLRDQITAGASAVQLFDSWAGALSPPVYE
                     RHVLPHSRKVFDAIADLDVPRIHFGVGTGEILGLMGDAGADVVGIDWRVPLDVARTRV
                     PGKAVQGNLDPAVTFAGWDATRAAVDDVLARNGGQAGHVFNLGHGVLPNTDPDILARV
                     VDHVHEHGRADGAAR"
     gene            1394..1657
                     /locus_tag="VLR27_02365"
     CDS             1394..1657
                     /locus_tag="VLR27_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02314.1"
                     /translation="MRRSIDDHPATGSDMPPGWEELDLQQVSWAVAISDDYDDAEPRV
                     VLTTEEVGRPGTGLVTHLAPGVARRLRRALHDALVEIGEDPGS"
     gene            1734..3899
                     /locus_tag="VLR27_02370"
     CDS             1734..3899
                     /locus_tag="VLR27_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HSP02315.1"
                     /translation="MPETTPSTPPTPRRDPVDRERHGRTAVDEYAWMRDDERTDPDVI
                     AHLEAENAHTAAVLEPTKDLQEAIFAEIKGRVQETDLSVPTRRGAWWYYSRTIEGQQY
                     PVHCRLAAGDETDPPDPDDPSVVDAEEVLLDQNAEAATDPANDYFSLGVFDLSPDDGT
                     LAWAADRNGSERHLLRFRDLSTGEDRRLPDGSLDEVPGVYYSTAWSADGGMFFYTRPD
                     EATRPHEVWRHRLGTPAAEDVLVHREDDERFFCQISETKDRQWLVLEMGSKITSEVRV
                     GPTVDPAEPFHVLVPRRQGIEAEVEHVGGTFFLLTNDEALDFRVASASSDERHLQAGP
                     DPSSWVDVVPHTPGVRLEGIDVLRDHLLVEERTEATTRIRVRRLSSGEEFLLDQAEEV
                     SAVSLGSNPDMGWPQLRHTYTSLVTPPTVIDVDLDAGTRTVRKVQPVLGGYDPEQYVS
                     RRLWATADDGTQVPISLVHRRYREPGGPVLLYGYGSYEISIDPGFSISRLSLLDRGWA
                     FAIGHIRGGGEMGRRWYLEGKLEAKANTFDDLIACGRALVDDGVAGTADDGRPRVAIR
                     GGSAGGLLVGAALNRAPEQWAAVVAEVPFVDALNTILDPTLPLTVIEWEEWGNPVDSP
                     EIFDVMGAYAPYENIPEVPLPPVLATGGLHDPRVGYWEPAKWVARLRDRAEGGPFLLK
                     TEMGAGHGGPSGRYDAWRDEAMVLAFLVTRVGEGTLASG"
     gene            3945..6125
                     /locus_tag="VLR27_02375"
     CDS             3945..6125
                     /locus_tag="VLR27_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02316.1"
                     /translation="MNEHPELSAEQAYVDHAYDCLETARDRATRLRSMVEVGKGGTEQ
                     ARWEREMIEGSIATRLTQLDLGDSSLVFGRLDTDADHGDDSFYIGRMSVADDEQEPVV
                     VDWRAPISEAFYRATGRQPMGLARRRHFTARGRTLLNLEDELFGEAVHGDHGGIRLAD
                     DDRIQGHGALIRALETTRTGRLGDIVATIQGEQDEIIRSELSGVLVVQGGPGTGKTVV
                     ALHRAAYLLYTHRFPLEGQGVLVLGPNRLFLGYIDQVLPSLGEAGVELAVLADLVDRE
                     RIDGEDQPRVARVKGDARMAHVIRKAVADRQRQLRSDLRVGLGAQTLVCTVDDSARIV
                     ADAHRRYHRHNAGRKHVEQRLFEVLADSARNPVRAEDVRDQLRRHPAVIEALEWMWPV
                     LTPAQLLHDLFGSRALIELASRKWLRPEEQAELHRPRTSIDEVTFTWQDVPLLDEARE
                     RLGPRRKRKGQDDEIRTYGHIVVDEAQDLSPMQLRMVARRSLSGSLTIVGDIAQATGA
                     WAHESWEEILEHLPTKRGTRQAELTVGYRIPAKNMEMATRVLRVAAPELTPPISVREG
                     GDPPIVRHAETGGLHQLVAEVTRAEAAEVDPGSLAIIVPATFVEQVSEALTDAGIEHG
                     RATRQGLRQPVTIVPVGMVKGLEVDASVVVEPAAIVDEEAQGLRALYVALTRATKRLA
                     IVHERPLPDFLAEPGDAVDDGAAPASLNGHDSQLRLDLPVEEAG"
     gene            6125..>6438
                     /locus_tag="VLR27_02380"
     CDS             6125..>6438
                     /locus_tag="VLR27_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSP02317.1"
                     /translation="MSEITARGITVSFDGTEVLHGVDLDVAEGSVVALLGPSGCGKTT
                     LLRSIAGLERPTGGEVRIGDTLVSGPGTQVPPERRRIGMVFQDWALFPHLTVAGNVSF
                     GLP"
BASE COUNT          921 a         2429 c         2272 g          816 t
ORIGIN      
        1 ggctgctggc ggggtgcacg agctcgtcga tgccgatctc ggcgcacagc tcgagcgcgt
       61 ccggcacccg ggcgaggaac gcatccgcgc cctcctccag cgggaacgcg aggccccgca
      121 cccgggagcg ctggagcttg ccgccgatct cccgggccga ttcgaacacg acgacgtcgt
      181 gcccggcgcg ctcgagctcc cacgccgccg agagcccggc gatgccggcg ccgatgacgg
      241 cgaagcgcgc catcagcggg cggcgccgtc ggctcgaccg tgctcgtgga cgtggtcgac
      301 cacgcgagcg aggatgtcgg ggtcggtgtt gggcagcacg ccgtgcccga ggttgaacac
      361 gtggccggcc tgcccgccgt tgcgggcgag cacgtcgtcg acggctgccc tcgtggcgtc
      421 ccagccggcg aaggtgacgg ccgggtcgag gttgccctgc accgccttgc cgggcacccg
      481 ggtgcgggcc acgtcgaggg gcacccgcca gtcgatgccg accacgtcgg cgccggcgtc
      541 gcccatcagc ccgaggatct cgccggtgcc cacgccgaag tggatgcggg gcacgtcgag
      601 gtcggcgatg gcgtcgaaga ccttgcggga gtggggtagc acgtggcgct cgtagacggg
      661 cgggctcagc gcgccggccc aggagtcgaa cagctgcacg gcgctcgcgc cggcggtgat
      721 ctggtcgcgc agcgacgcga tggcgatgtc ggccaggcgg tcgagcaggg cggcgaacag
      781 ctcgggctcg gcgaacatca tccgcttggt gtgctcgtag gtgcggctgg ggcggccctc
      841 gatgaggtag ctggcgacgg tgaagggagc gccggcgaag ccgatgaggg gccggtcgcc
      901 gagctcgtcg acgaggatgc ggaccgtctc ggcgatgtag ggcgagtgct cctcgggcac
      961 gagcacgggg agccgctcga ggtcctcctt cgtccggaag ggctgctcgg ccaccggacc
     1021 gacgccggcg acgatgtcga tgccgaagcc gatggcccgg gccggcacga cgatgtcgct
     1081 gtagaagatg gcggcgtcca cgccgtagcg gtgcaccggc tgcatcgtca gctcggcggc
     1141 cagctcggga cgttccacgg cgtcgaggat cgagacgtcg cccttgacct ggttgtactc
     1201 gggcagcgac cgcccggcct ggcgcatgaa ccacaccggc acgtgctcgg cgggcaggcc
     1261 ccggcatgcc tgcaggaacg ggtggtcggg gcgcggcgcc atggaccgaa cctactgacg
     1321 ggcccagcgt ccggtcctgc cggcaccccc ggagcttctg gcagcggcca cggaacacga
     1381 ccatgctcgt ggcgtgcgcc gctccatcga cgaccacccc gccaccggtt ccgacatgcc
     1441 gccgggctgg gaggagctcg acctgcagca ggtgtcgtgg gccgtggcga tcagcgacga
     1501 ctacgacgac gccgaacccc gcgtcgtgct gaccaccgag gaggtcggcc gaccgggcac
     1561 cggtctggtc acgcacctcg cgccgggcgt ggcccggcgg ttgcgacgtg ccctgcacga
     1621 cgcgctggtc gagatcggcg aggaccccgg cagctgacgt tccgccgtcg ctgccagaag
     1681 cgggggagtg cgacgggatc gcgtccagaa ccgtgcggga tagcgtccgg cgggtgcctg
     1741 agacgacccc gtcgaccccg cccacgcccc gacgcgaccc ggtggaccgg gagcgccacg
     1801 gccgcacggc cgtcgacgag tacgcctgga tgcgcgacga cgagcgcacc gatcccgatg
     1861 tcatcgccca cctcgaggcc gagaacgccc acaccgcagc ggtcctcgag ccgacgaagg
     1921 acctgcagga ggcgatcttc gccgagatca agggccgggt gcaggagacc gacctgtccg
     1981 tgcccacccg gcgcggcgcg tggtggtact acagccgcac catcgaaggg cagcagtacc
     2041 cggtgcactg ccgactcgcc gccggcgacg agaccgaccc gcccgacccc gacgacccgt
     2101 cggtcgtcga cgccgaggag gtgctgctcg accagaacgc cgaggcagcc accgacccgg
     2161 cgaacgacta cttctccctc ggcgtgttcg acctgtcgcc cgacgacggc acgctcgcct
     2221 gggcggcgga ccgcaacggc tccgaacggc acctgctccg cttccgggac ctgtcgaccg
     2281 gcgaggaccg gcggctgccc gacggctccc tcgacgaggt gcccggcgtc tactactcca
     2341 cggcgtggtc ggccgacggg ggcatgttct tctacacgcg ccccgacgag gccacccgcc
     2401 cgcacgaggt gtggcgccac cgcctcggca cgccggcggc ggaggacgtg ctcgtccacc
     2461 gcgaggacga cgagcgcttc ttctgccaga tcagcgagac caaggaccgg cagtggctgg
     2521 tgctcgagat gggatccaag atcaccagcg aggtccgggt cgggcccacg gtcgatccgg
     2581 ccgagccctt ccacgtgctg gtgccgcgtc ggcaggggat cgaggccgag gtcgagcacg
     2641 tcggcggcac cttcttcctc ctgaccaacg acgaggccct cgacttccgg gtcgcgtccg
     2701 cctcgtcgga cgagcggcac ctgcaggccg gccccgatcc atcctcgtgg gtcgacgtcg
     2761 tcccgcacac gcccggcgtg cgcctcgagg gcatcgacgt cctgcgcgac cacctgctgg
     2821 tggaggagcg caccgaggcc accacccgca tccgggtgcg ccggctgtcg agcggcgagg
     2881 agttcctgct cgaccaggcc gaggaggtgt cggccgtgtc gctggggtcg aaccccgaca
     2941 tgggctggcc ccagctccgc cacacctaca cctcgctcgt gaccccgccc acggtgatcg
     3001 acgtcgacct cgacgccggc acccgcaccg tgcgcaaggt gcagccggtg ctcggcggct
     3061 acgacccgga gcagtacgtc tcgcggcgac tgtgggccac cgccgacgac ggcacgcagg
     3121 tgcccatcag cctcgtgcac cggcgctacc gcgagcccgg cggcccggtg ctgctgtacg
     3181 gctacggctc ctacgagatc tccatcgacc cgggcttctc gatctcccgg ctgtccctgc
     3241 tcgaccgcgg gtgggcgttc gccatcggcc acatccgcgg cggcggggag atgggtcgcc
     3301 gctggtacct cgagggcaag ctcgaggcga aggccaacac cttcgacgac ctgatcgcct
     3361 gcggacgggc actggtcgac gacggcgtgg ccggcaccgc cgacgacgga cgcccccgcg
     3421 tcgccatccg gggcggttcc gccggcggcc tgctcgtcgg cgccgccctc aaccgggcgc
     3481 ccgagcagtg ggccgcggtg gtggccgagg tcccgttcgt cgacgcgctc aacaccatcc
     3541 tcgacccgac cctgccgctc accgtcatcg agtgggagga gtggggcaac ccggtcgact
     3601 cgccggagat cttcgacgtc atgggggcct acgcgcccta cgagaacatc cccgaggtgc
     3661 ccctcccgcc cgtgctcgcc accggcgggc tccacgaccc ccgggtcggc tactgggagc
     3721 cggccaagtg ggtggcccgg ctgcgcgacc gcgccgaggg cgggccgttc ctgctgaaga
     3781 ccgagatggg cgccggccac ggcggtccgt cggggcgcta cgacgcctgg cgggacgagg
     3841 cgatggtgct ggcgttcctc gtcaccaggg tgggggaggg gaccctcgcg tcggggtagc
     3901 atgttcgatc cctccgaacc agcctcgaag gtgatcctgc gccgttgaac gagcatccgg
     3961 aactgagtgc cgaacaggcc tacgtcgacc acgcgtacga ctgcctggag acagcgcgcg
     4021 accgggccac gcggctgcgc tcgatggtcg aggtcggcaa gggcggcacc gagcaggccc
     4081 gctgggagcg ggagatgatc gagggcagca tcgccacgcg cctgacccag ctcgacctcg
     4141 gtgacagctc gctggtcttc ggtcgcctcg acaccgacgc cgaccacggc gacgactcct
     4201 tctacatcgg gcgcatgtcc gtggccgacg acgagcagga gcccgtggtc gtcgactggc
     4261 gggcgcccat ctcggaggcc ttctaccggg ccacgggacg ccagcccatg ggcctggccc
     4321 gtcgccgcca cttcaccgcc cggggtcgca ccctgctcaa cctcgaagac gagctgttcg
     4381 gcgaagccgt ccacggggac cacggcggca tccgcctggc cgacgacgac cgcatccagg
     4441 gccacggcgc cctcatccgg gccctcgaga ccacccgcac cggccggctc ggcgacatcg
     4501 tcgccaccat ccagggcgag caggacgaga tcatccgctc cgagctctcc ggcgtgctcg
     4561 tcgtgcaggg cggccccggc acgggcaaga ccgtcgtcgc gctgcaccgc gccgcctacc
     4621 tgctctacac gcaccgcttc cccctcgagg gacagggcgt gctggtgctc ggccccaacc
     4681 gcctcttcct cggctacatc gaccaggtcc tgccctcgct cggcgaggcc ggggtcgagc
     4741 tcgccgtcct cgccgacctc gtcgaccgcg agcgcatcga cggcgaggac cagccccggg
     4801 tcgcccgggt caagggcgac gcccggatgg cccatgtgat ccgcaaggcc gtcgccgacc
     4861 gccagcgcca gctgcgcagc gacctgcgcg tcggcctcgg cgcccagacg ctggtgtgca
     4921 ccgtggacga cagcgcccgc atcgtggccg acgcccaccg ccgctaccac cgccacaacg
     4981 ccggccggaa gcacgtcgag cagcgcctgt tcgaggtcct ggccgactcg gcgcgcaacc
     5041 cggtccgggc cgaggacgtg cgcgaccagc tgcgccgcca ccccgccgtg atcgaggccc
     5101 tcgagtggat gtggccggtg ctcacgccgg cccagctgct ccacgacctg ttcgggtccc
     5161 gggcgctcat cgagctggcc agccggaagt ggctgcggcc cgaggagcag gccgagctgc
     5221 accgtccccg cacctccatc gacgaggtga ccttcacctg gcaggacgta ccgctgctcg
     5281 acgaggcccg cgagcgcctc ggcccccgcc gcaagcgcaa gggccaggac gacgagatcc
     5341 gcacctacgg ccacatcgtc gtcgacgagg cccaggacct gtcgccgatg cagctgcgca
     5401 tggtcgcccg ccgctcgctg tcggggtcgc tgaccatcgt cggcgacatc gcccaggcca
     5461 ccggggcgtg ggcgcacgag agctgggagg agatcctcga gcacctcccc accaagcgcg
     5521 gcacccggca ggcggagctc accgtcggct accgcatccc ggccaagaac atggagatgg
     5581 ccacccgggt cctgcgcgtc gccgcccccg agctcacccc gcccatctcg gtccgcgagg
     5641 gcggcgaccc gcccatcgtc cgccacgccg agaccggcgg gctccaccag ctcgtcgccg
     5701 aggtcacccg ggccgaggcc gccgaggtcg accccggctc cctcgccatc atcgtcccgg
     5761 ccaccttcgt cgagcaggtg tccgaggcgc tcaccgacgc cggcatcgag cacggccggg
     5821 ccacccgcca gggactgcgc cagccggtca cgatcgtgcc cgtgggcatg gtgaagggcc
     5881 tcgaggtgga tgcatccgtc gtggtcgagc cggcagccat cgtcgacgag gaggcccagg
     5941 gcctgcgcgc cctctacgtg gcgctgaccc gcgccaccaa gcgcctggcc atcgtgcacg
     6001 agcgcccgtt gccggacttc ctcgccgagc ccggcgacgc ggtcgacgac ggggccgccc
     6061 cggcgtcact gaacggccac gacagccagc tgcgcctcga cctgccggtg gaggaggcgg
     6121 gctgatgagc gagatcaccg cccgcggcat caccgtctcc ttcgacggca ccgaggtgct
     6181 ccacggcgtg gacctcgacg tggccgaggg cagcgtcgtg gccctgctcg gcccgtccgg
     6241 atgcggcaag accaccctgc tgcggtccat cgccggcctc gagcgcccca ccggcggcga
     6301 ggtccgcatc ggcgacaccc tcgtcagcgg ccccggcacc caggtcccgc cggagcggcg
     6361 ccgcatcggc atggtgttcc aggactgggc gctgttcccg cacctcaccg tcgccggcaa
     6421 cgtgtccttc ggcctgcc
//
LOCUS       DATISU010000053         1851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_701277, whole genome shotgun sequence.
ACCESSION   DATISU010000053 DATISU010000000
VERSION     DATISU010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701277"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..969)
                     /locus_tag="VLR27_02385"
     CDS             complement(31..969)
                     /locus_tag="VLR27_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSP02318.1"
                     /translation="MAEACERVANQVESVIMGKRPVIDLILAALLADGHILLEDVPGV
                     GKTSLAKALAASIEGTFGRVQFTPDLLPTDVSGVTIYRRGRDEFEFRPGPIFANIVLA
                     DEINRASPKTQSALLEAMAERQVTVDGSTYPLAAPFLVIATQNPIEHEGTFPLPDSQL
                     DRFLVRIEVGYPDVDAELTMLDSHGSVDPLLSVRPVLHTEDVLTMRRSAAAVHVAPAL
                     KQYLVQLADRTRAHPQLELGMSPRATLSFLRIARVWAAMRDRDYATPDDVKALAVPVL
                     AHRLLVTPEADLQGVTAADVVADVLDSVPVPRPRSA"
     gene            1142..>1851
                     /locus_tag="VLR27_02390"
     CDS             1142..>1851
                     /locus_tag="VLR27_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSP02319.1"
                     /translation="MATLAIDIGGTKLAAGVVDAVGALAHRAQRTTAATGDGEALFAD
                     LVGLADAVLVDARRAGLEIEACGVGSGGPMTAGGASVSPLNIPAWRGFPLRDRLAEHL
                     DLAVAVDNDAKALALGEGWCGAAAGERDFLAMVVSTGVGGGIVSRGRLLEGASGTAGH
                     VGHVFVADGTEAWPRHPAGLLASEASGTAIAQRTGAPAADAGVDVRSRTGTLVGRAVA
                     SVANLLDLRLAVVAGSVAL"
BASE COUNT          216 a          633 c          751 g          251 t
ORIGIN      
        1 caacccggtg cgggtggggc gcacggctcg tcaggccgac cggggtcggg ggacggggac
       61 gctgtcgagc acgtcggcca ccacgtcggc cgcggtgacg ccctgcaggt cggcctccgg
      121 ggtgacgagc aggcggtggg ccagcaccgg gacggccagc gccttgacgt cgtcgggcgt
      181 ggcgtagtcg cggtcgcgca tggcagccca cacccgggcg atgcgcagga agctgagggt
      241 ggctcgcggc gacatgccca gctcgagctg ggggtgggcg cgggtgcggt cggccagctg
      301 caccaggtac tgcttgagcg ccggtgccac gtgcaccgct gcggccgacc ggcgcatcgt
      361 gaggacgtcc tcggtgtgca ggaccggccg gaccgacagc agcgggtcga ccgagccgtg
      421 gctgtcgagc atggtgagct cggcgtcgac gtcggggtag ccgacctcga tgcgcacgag
      481 gaaccggtcg agctgggagt cgggcagcgg gaaggtgccc tcgtgctcga tcgggttctg
      541 ggtggcgatc accaggaacg gggcggcgag cgggtaggtc gacccgtcga cggtgacctg
      601 gcgctcggcc atggcctcga gcagcgccga ctgggtcttg ggcgatgccc ggttgatctc
      661 gtcggcgagg acgatgttgg cgaagatcgg tccgggccgg aactcgaact cgtcccgacc
      721 ccggcggtag atggtgacgc cggagacgtc ggtggggagc aggtcggggg tgaactgcac
      781 ccggccgaac gtgccctcga tggaggcggc gagggccttg gccaggctgg tcttgccgac
      841 gcccggcacg tcctcgagga ggatgtggcc gtcggcgagg agggcggcga ggatcaggtc
      901 gatcacgggc cgcttgccca tgatcaccga ctcgacctgg ttggcgacgc gctcgcacgc
      961 ctcggccacc gagctccctc gcaacgcctc tgtctggctg ctcgccaccc gtcctcctgg
     1021 tcgtctgcgt cgtcgtggca cggccgacgt cggtgcgacc cgccgcctgg gcagactacg
     1081 gcgccgggat cccgcgtgga tgctcggggc cgagggtgcc gggctcccta cgatcgcccg
     1141 ggtggcgacc ctcgcgatcg acatcggcgg caccaagctg gcggcgggcg tggtcgacgc
     1201 cgtgggcgcc ctggcgcacc gagcccagcg gaccaccgcc gcgacggggg acggcgaggc
     1261 cctcttcgcc gacctcgtcg ggttggccga cgccgtcctc gtcgacgcca ggcgcgccgg
     1321 cttggagatc gaggcgtgcg gggtgggctc cggcgggccc atgaccgccg gcggcgcgtc
     1381 ggtgtcgccg ctgaacatcc cggcctggcg cgggttcccg ctgcgggacc gcctggccga
     1441 gcacctggac ctggcggtcg cggtggacaa cgacgccaag gcgctcgccc tcggggaggg
     1501 ctggtgcggt gcggcggcgg gggagcggga cttcctcgcc atggtcgtgt ccaccggcgt
     1561 gggcgggggg atcgtgagcc gcggccggct gctcgagggc gcgtcgggca ccgccggcca
     1621 cgtcgggcac gtcttcgttg ccgacggcac cgaggcctgg ccgcggcacc ccgccggact
     1681 gctcgcgtcc gaggccagcg gcacggccat cgcccagcgc accggcgccc cggccgccga
     1741 cgccggtgtc gacgtccggt cccggacggg cacgctggtg ggtcgggcgg tcgcgtcggt
     1801 ggccaacctg ctcgacctgc gcctggccgt ggtcgccggg tcggtcgccc t
//
LOCUS       DATISU010000054        11098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_95772, whole genome shotgun sequence.
ACCESSION   DATISU010000054 DATISU010000000
VERSION     DATISU010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 11098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11098
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95772"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..176
                     /locus_tag="VLR27_02395"
     CDS             39..176
                     /locus_tag="VLR27_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02320.1"
                     /translation="MQAQFFFSRSETILGGTDEVQRNIVGERVLGLPKEPKPENELPN
                     G"
     gene            complement(190..1167)
                     /locus_tag="VLR27_02400"
     CDS             complement(190..1167)
                     /locus_tag="VLR27_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HSP02321.1"
                     /translation="MSHRNARLTVHGRRLLVSRVRQQGMPVAHVAKAMGVSRKCAYHW
                     LRRFDLEGDAGLHDRSSRPHRMPTRTPPEVEARVVAARAEHRRGQDWLGPEVGVPART
                     VGRILRRHDVPRLASCDPMTGEVIRASKTTAVRYERDRPGELVHMDVKKIGRIPDGGG
                     WRAHGRQAGSTSRDRKRRVGFDFVHSLVDDHSRLAYSEILDDEKAGTCAGFFARAVDY
                     FASFGIDRIEALMTDNHWSYTNSNALARQLQDLGAKHITIRPHCPWQNGKVERFNRTL
                     QSEWAYRQAFHSNAERTAALDPWLDNYNTQRRHSALGGLPPISRLPSPT"
     gene            complement(1264..3264)
                     /locus_tag="VLR27_02405"
     CDS             complement(1264..3264)
                     /locus_tag="VLR27_02405"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSP02322.1"
                     /translation="MNRLAQESSPYLRQHADNPVHWQPWGPEAMAEAADRDVPILLSV
                     GYSACHWCHVMAHESFEDDATAAVMNEHFVNVKVDREERPDVDAVYMEAVQAMTGRGG
                     WPMTVFLTPDGRPFYGGTYFPPTRHGQMPSFVELLEAVHDTWSTKRDQLFEQADRLTE
                     ALGQGGRMQAGAEVPGPEVLEHAINHLHQAFDEEWGGFGGAPKFPTPQNLDFVLRGLA
                     RTGVEELATIAETTLDAMASGGMYDHLGGGFARYSVDPHWVVPHFEKMLYDQSGLVRL
                     YLHGHLVTGHARYAQVVEETVGYVLRDLRQPGGGIASAEDADSLDASGHSEEGAFYTF
                     TPDEVRTALVDAGLGDVVDDALTWWELDRPANFEGRWIPVRTNHRGDWDRPEPIERAR
                     QALFDARATRPRPGLDDKVLTEWNAHWVSALAEAGFALGRRDWVDAAVETAEFLLSSL
                     RRDDGRWLRSWQAETGAKHLAFAGDHAALVDAFTRLAEATGQARWIAEARAVADAMLE
                     LFWDTDQGGLFTTGSDGEQLVARDKDLQDQALPSANSTAAVALLRLAALTGEHRYTGP
                     AAGILRLSGELAARVPSAFGVLLGAIDLHHTGTTEIVVAGDRPDLLDAVRHRWMPNAV
                     LAWGERYESPLWTGRDDGRAYVCRDYTCQLPASTPEELTAQL"
     gene            3385..4317
                     /locus_tag="VLR27_02410"
     CDS             3385..4317
                     /locus_tag="VLR27_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HSP02323.1"
                     /translation="MASPRVLTVTVNPALDVSVSVEYLVPDHKLDALDHARELGGGGV
                     NVARVLGRLGVESRAWMAVGGAVGDELLSLAAGEGIDTAPHRIDGMTRESIAVSDRKT
                     GRQYRIVLPGPSITDHQSMVADIEALAADADVVVLSGGLPPGVPEDVYRTLCDDLEHV
                     TTIVDAKGAALARTVEGSADVVKPSRRELASLVSWIPVDLAEVELAARQVLDRGRVGA
                     LAVSLGGDGALLVERDGPTTRYAAPEVEVVSTVGAGDSMVAGLVAGLVLGEPLVEATR
                     RGVAAGSAATMTPGSDLCHRPDVDALLPRVQVTG"
     gene            complement(4350..4979)
                     /locus_tag="VLR27_02415"
     CDS             complement(4350..4979)
                     /locus_tag="VLR27_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP02324.1"
                     /translation="MPVPRQEEILQRGRVSQKRRTRAAIVDAARGLLAEGTTPTVSQA
                     AAAAEVSRTTAYRYFPTQDSLLVEVAMNADVDDIEELVARDVTPEDARARALEVLDLF
                     NRHVADAEVEYRTALRLYLDQWLREKEAGDDAPQVREGRRRRWFEHSLEPVRDTVAPE
                     QWERAITGLCMLSGPEALTVLRDVCHLDDEGGRAAARWAAETLLDATFG"
     gene            5036..5845
                     /locus_tag="VLR27_02420"
     CDS             5036..5845
                     /locus_tag="VLR27_02420"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSP02325.1"
                     /translation="MKTRLEPTRIAPETFLIHDHAGEGTAPVSIALNAMLIRGEQPVV
                     VDTGAVEHREQYLDDLFSLVEPEDVRWVFISHDDVDHTGNLNAVMAACPNATLVIDWF
                     MQERMGKTLEVSPLRWRWMQDGESLDVGDRQLQLVRPPVYDSPTTRGLFDPTTGVYWA
                     ADGFATPMPVPVRDVADIDLEVWTAGMATFNQYISPWLLLADERRYQGTVDRIAALQP
                     TAIAGCHTPVIVGDRVAGAIDITRRTPGETVPAQPDQAVLDQIQRTLVEAA"
     gene            complement(5873..7231)
                     /locus_tag="VLR27_02425"
     CDS             complement(5873..7231)
                     /locus_tag="VLR27_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HSP02326.1"
                     /translation="MQPTTTSPRPLPHPPWRAQPAYLRRIFQDPQPVLDELRERYGPV
                     VGLGAGPVRMAIVGDPAALKELFATSTDSFRWNHPFNVLAFVVGPGSMIVSDGDDHKR
                     RRRSVQAAFSRRRLDGWIPLILERTDAAIDRVVADGDHVRDLYVTGRELVLDLVVRIL
                     FGERMAHRTAEIGALFQGPQDYLEAPAIRQVPHPIPGTTRARVRADRKALDRIIDAEI
                     AERRARPSGDRLDVLEALVVDGTLDDSEIRDQVVTLIGAGYDTTSASLAWLLWCTTLA
                     DGVWDRLRAEADDVLGPVGSGPAPDASTLRALDLASRVVRETTRLHPAGVVSPRQAAV
                     DLVLGGHEIPAGTLVLWSAHLAGRDPSAWDDPLRFDPDRFLDLSPERQALADAAWVPF
                     GKGARNCIGFALAQMELTLIAARLAQRLDVEPTAPVEPRPVGMVVNRPAGGAPMRVRP
                     RV"
     gene            complement(7285..8493)
                     /locus_tag="VLR27_02430"
     CDS             complement(7285..8493)
                     /locus_tag="VLR27_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP02327.1"
                     /translation="MSDGRPVGATSVVRLVARREVVERLRARSFAVATGLLVVVILAI
                     GIISRLNSDDGADAIDVAVAGAQPTGFEEMTGQTGALFERTVEITAFDDADAARTAVA
                     EGEADVAVIGDRGEIVFDEDIAQQVLAILQQSWSSAQVQEALADAGLDEAEVTEALTT
                     TQLDPVTLDGDDDGNEGVALLTGSLAGILLFISLQTFGTYVLTGVVEEKATAVVEVLL
                     VRARGSEMLAGKVIGIGMAALLQFALAIVAALVSLAISGVEVPGEVWSALPLTLVWFL
                     GGYALYSTLFALAGSLVSRQEDAQAAAMPIMSVLLLAYVMVFTFGSDPESTGSTVMSM
                     LPPIAPLLMPTRMAAGAASVLEVGVALVLLVLAILAAWAATGRIYEQVLLRRGTRISW
                     KDAAALIRRT"
     gene            complement(8490..9416)
                     /locus_tag="VLR27_02435"
     CDS             complement(8490..9416)
                     /locus_tag="VLR27_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSP02328.1"
                     /translation="MLCVRGLTKRYGPLLALDDVDFDVPRGQIVGFLGPNGSGKTTTM
                     RAIMRLIHLDAGSVTWDGVPVDPDVRQHFGYMPAERGMYPRMKLRDHLSYYGELSGLT
                     SGRAGEAADRWLERLGLDDRADDTIQSLSSGNQQRVQLALALLNDPDLLVLDEPFSGL
                     DPVAVEMLSDILREQVDRGAALLLSSHQLDLVADVCSAVVIVDRGRIVLRGEVAALRA
                     ASPTRYVEVEFEEAVRWDAGRDSEVTAGGRRHRVAVEAGTDADRLLADARSLAPVVGY
                     SFLPPDLSEVFLGAVGRDTVEDPTHERREVTG"
     gene            complement(9471..10637)
                     /locus_tag="VLR27_02440"
     CDS             complement(9471..10637)
                     /locus_tag="VLR27_02440"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSP02329.1"
                     /translation="MPHPRLAIVATIAAALLATACGASSNAAPSVDLPLPAPRAPEEP
                     VGDAPALALGPLIETTPASALADPPGPGPVLVSTLRGEVLAVDLDAGTTEVVMDLSER
                     ISSGGEQGLLGMAIDPDGDRVYLDYTALDDDTEIRSWPLDDDGMPIADGDGVLHLEIE
                     QPTDTHNGGHLVFGPDGALWIATGDGGGVGDPDGLARDERSLLGKMLRVVPDPDGGIR
                     TVGSNPDWQRPEIWGIGLRNPWRWSFDRHTNRMWVADVGQWVLEEVSVVDPDEARPDF
                     GWSEVEGTLPFEDGERSDAFIEPALTYGRDDGCAVTGGYVYRGSENPGLHGWYLFGDF
                     CQAWIRAVPSDDPGAEPVELIDRISSVTSFGELEDGELVALTVAGIHRLLPRPG"
     gene            10688..>11098
                     /locus_tag="VLR27_02445"
     CDS             10688..>11098
                     /locus_tag="VLR27_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSP02330.1"
                     /translation="MEIRLDGQVALVTGASKGIGKAIAQTLAEAGAKVVLSSRKQDAL
                     EAAAADIDGETSVFAANAGDPDAAAACVAHTMETFGRVDILVNNAATNPYMGRPIEID
                     LPRYDKTWEVNLRGPLVWAQECWKASMQDNGGAVI"
BASE COUNT         1582 a         3950 c         4024 g         1542 t
ORIGIN      
        1 gcaagcgcga cgccgcccgg gagtacccgg tctcgccgct gcaggcccag ttcttcttct
       61 cccgctccga gacgatcttg ggcggcaccg acgaggtgca gcgcaacatc gtgggggagc
      121 gggtcctggg cctgcccaag gagcccaagc cggagaacga gctgcccaac ggctagttgt
      181 acccggccat caggttggtg acggaagccg gctgatcggc gggagcccgc caagggcgct
      241 gtggcgtcgt tgagtgttgt agttgtcgag ccaggggtcc agcgctgcgg ttcgttcggc
      301 gttgctgtga aaggcctgcc ggtacgccca ctcgctctgc aaggtccggt tgaagcgctc
      361 caccttgccg ttctgccacg gacagtgcgg ccggatcgtg atgtgcttgg cgccgagatc
      421 ctggagctgg cgggcgaggg cgttgctgtt ggtgtagctc cagtggttgt cggtcatgag
      481 cgcttcgatt cggtcgatgc cgaaggaggc gaagtagtcg accgcccggg cgaagaaccc
      541 agcgcaggtc ccggcctttt cgtcgtcgag gatctcggag tacgccaggc ggctgtggtc
      601 gtcgacgagt gagtgcacga agtcgaaccc gactcgtcgt ttgcggtccc gtgaggtcga
      661 gccggcctgt cggccgtggg cccgccagcc gccgccgtcg gggatccgcc cgatcttctt
      721 gacgtccatg tgcacgagct ccccggggcg atctcgctcg tagcgcacgg cggttgtctt
      781 cgacgcccgg atcacttcac cggtcattgg gtcacacgac gccaaccgcg ggacgtcgtg
      841 acgacgcaag atccgaccga ccgtgcgggc tgggacgccg acctcggggc cgagccagtc
      901 ctggccacgg cgatgctcgg cccgagcggc aaccactctg gcttcgacct cgggcggtgt
      961 cctcgtcggc atccgatgcg gccgcgacga ccggtcgtgc agcccggcat cgccctcgag
     1021 atcgaagcgc cgcaaccagt gataggcgca cttcctcgac acgcccatcg ctttcgcgac
     1081 atgggcaacc ggcatgcctt gctgacgaac tcgggagacg aggagtcggc gaccatgcac
     1141 ggtcaaccga gcgttacggt gagacacgaa gacctcctgg gatgtgaaga cggcaatctc
     1201 cacagtgccc cggaggtctt cgctatctca tcgacctgtc accaacgtcc gggccgggta
     1261 cacctagagc tgggcggtga gctcctcggg ggtcgacgcc ggcagctggc acgtgtagtc
     1321 ccggcagacg taggcccggc cgtcgtcgcg gcccgtccag aggggcgact cgtagcgctc
     1381 gccccaggcg agcacggcgt tgggcatcca ccggtgccgg acggcgtcca gcaggtcggg
     1441 gcggtcgccg gcgacgacga tctccgtggt gcccgtgtgg tgcaggtcga tggcgccgag
     1501 cagcacgccg aaggcggacg gcacccgggc ggccagctcg ccggagaggc gcaggatgcc
     1561 ggcggcgggt ccggtgtagc ggtgctcgcc ggtgagcgcg gcgaggcgca ggagcgccac
     1621 ggccgcggtc gagttcgccg atggcagcgc ctggtcctgg aggtccttgt cccgcgcgac
     1681 gagctgctcg ccgtcgctcc cggtggtgaa cagcccgccc tggtcggtgt cccagaacag
     1741 ctcgagcatg gcgtcggcca ccgctcgggc ctcggcgatc cagcgggcct gccccgtcgc
     1801 ctcggccagg cgggtgaagg catcgacgag ggcggcgtgg tcgccggcga aggcgaggtg
     1861 cttggcgccg gtctcggcct gccacgagcg cagccagcga ccgtcgtccc gacgcagcga
     1921 ggacagcagg aactccgccg tctcgaccgc ggcgtcgacc cagtcccggc gtccgagcgc
     1981 gaacccggcc tcagcgaggg cggacaccca gtgggcgttc cactcggtga gcaccttgtc
     2041 gtccagcccc ggccgggggc gggtggcccg ggcgtcgaac agggcctggc gggctcgctc
     2101 gatgggctcg ggccggtccc agtcgccgcg gtggttggtg cgcaccggga tccagcggcc
     2161 ctcgaagttg gccggccggt cgagctccca ccaggtgagc gcgtcgtcga cgacgtctcc
     2221 gagcccggcg tcgacgagcg cagtccggac ctcgtcgggg gtgaacgtgt agaacgcgcc
     2281 ctcctcgctg tggccggagg cgtcgaggga gtcggcgtcc tcggccgatg cgatgccccc
     2341 accgggctgg cgcaggtcgc gcaggacgta gcccaccgtc tcctcgacga cctgcgcgta
     2401 ccgggcgtgc ccggtcacca ggtgcccgtg caggtagagc cgcaccagcc cggactggtc
     2461 gtagagcatc ttctcgaagt gcggcaccac ccagtggggg tccaccgagt agcgggcgaa
     2521 gccgccgccc aggtggtcgt acatcccgcc ggacgccatg gcgtcgagcg tggtctcggc
     2581 gatggtggcc agctcctcga cgccggtgcg ggccaggccg cgcagcacga agtcgaggtt
     2641 ctggggggtc gggaacttcg gcgcaccacc gaacccaccc cactcctcgt cgaaggcctg
     2701 gtgcaggtgg ttgatggcgt gctccaggac ctctggcccg ggcacctcgg caccggcctg
     2761 catccgcccg ccctggccca gcgcctcggt gagccggtcg gcctgctcga agagctggtc
     2821 ccgcttggtc gaccacgtgt cgtgcacggc ctcgagcagc tcgacgaacg acggcatctg
     2881 gccgtggcgc gtcggcggga agtaggtgcc gccgtagaag gggcggccgt ccggcgtgag
     2941 gaacaccgtc atgggccagc cgccgcgccc ggtcatggcc tggaccgcct ccatgtagac
     3001 agcatcgacg tcggggcgct cctcccgatc gaccttgacg ttcacgaagt gctcgttcat
     3061 caccgccgcg gtggcgtcgt cctcgaagga ctcgtgggcc atgacgtggc accagtggca
     3121 ggccgagtac ccgaccgaca gcaggatggg gacgtcgcga tcggccgcct cggccatggc
     3181 ctcaggtccc cacggctgcc agtgcaccgg gttgtctgcg tgctggcgga ggtaggggct
     3241 ggactcctgg gcgaggcggt tcatgccccc acccttccca ccggggccga tcctcgcaac
     3301 gtgctggtcg acgatcgtcg cgacaggcgc gccgcgtcgc cgccgtaggg tgcccctcgc
     3361 aggccctgcg gtgagaggag tgacgtggca tcgccccgcg tgctgacggt gaccgtcaac
     3421 ccggcactcg acgtgtcggt gtcggtggag tacctcgtcc ccgaccacaa gctcgacgcg
     3481 ctcgaccacg cccgcgagct cggcggcggt ggcgtgaacg tcgctcgtgt cctgggccgt
     3541 ctcggcgtgg agagccgggc gtggatggca gtcggcgggg cggtcggtga cgagctcctg
     3601 tccctggcgg caggtgaggg catcgacacg gcaccgcacc gcatcgacgg gatgacgcgg
     3661 gagtcgatcg cggtcagcga tcgcaagacc ggtcgccagt accgcatcgt cctccccggt
     3721 ccgtcgatca cggatcacca gtcgatggtg gccgacatcg aggcgctcgc cgccgacgcc
     3781 gacgtggtcg tgctctccgg cggcctccca cccggtgtgc ccgaggacgt ctaccgcacg
     3841 ctctgcgacg acctcgagca cgtcaccacg atcgtcgacg ccaagggagc cgcgctcgcc
     3901 cggaccgtcg aggggagcgc cgacgtcgtg aagcccagcc ggcgcgagct ggcatcgctg
     3961 gtcagctgga tccctgtcga cctcgccgaa gtggagctcg ccgcccgtca ggtcctggat
     4021 cggggccggg tgggcgcgct ggccgtgtcc ctcggcggtg acggcgccct gctcgtcgag
     4081 cgcgacggcc ccaccacccg gtacgccgcg ccggaggtcg aggtggtcag caccgtgggc
     4141 gccggcgatt ccatggtcgc cggcctcgtc gccggcctcg tcctgggcga gcccctcgtc
     4201 gaggccaccc gtcgcggcgt ggccgctggc tccgccgcca cgatgacgcc ggggagcgac
     4261 ctctgccacc gccccgacgt ggacgcgctg ctgccccggg tgcaggtcac cgggtgagcg
     4321 gcatcggcgc cgaggactga ccgcgaccgt cacccgaacg tcgcgtcgag cagcgtctcc
     4381 gccgcccacc gcgccgcagc gcggccgccc tcgtcgtcga ggtggcacac gtcgcggagc
     4441 acggtgagcg cctccggccc gctgagcatg cacagcccgg tgatcgctcg ctcccactgc
     4501 tccggcgcga cggtgtcccg caccggctcg aggctgtgct cgaaccatcg ccggcggcgt
     4561 ccctcgcgca cctgaggggc gtcgtcgccg gcctccttct cccgcagcca ctgatcgagg
     4621 tagagccgca gggccgtgcg gtactcgacc tccgcgtcgg ccacgtggcg gttgaacagg
     4681 tccaggacct cgagcgcacg cgcccgcgcg tcctccgggg tgacgtcccg ggcgacgagc
     4741 tcctcgatgt cgtccacgtc ggcgttcatg gcgacctcga ccaggagcga gtcctgcgtg
     4801 gggaagtacc ggtaggcggt cgtccgcgac acctcggccg ccgcagcggc ctggctcacc
     4861 gtcggcgtcg tgccctcggc gaggagcccc ctggcggcat cgacgatggc ggcgcgggtg
     4921 cggcgcttct ggctgacgcg accgcgctgc aggatttcct cttgacgtgg gacgggcatc
     4981 ccgtcatggt actccaatcc cacgacagtg caaagaaggg aagaccatgc acccgatgaa
     5041 gacccgcctc gagccgactc gcatcgcgcc cgagacgttc ctgatccacg accacgccgg
     5101 ggagggcacc gcaccggtgt ccatcgccct caacgccatg ctcatccgcg gggagcagcc
     5161 ggtcgtggtc gacaccggtg ccgtcgagca ccgggagcag tacctcgacg acctgttctc
     5221 cctcgtcgag cccgaagacg tgcgctgggt gttcatcagc cacgacgacg tcgaccacac
     5281 cggcaacctg aacgccgtga tggcggcgtg tcccaacgcg accctcgtga tcgactggtt
     5341 catgcaggag cgcatgggca agacactcga ggtgtcgccc ctgcggtggc gctggatgca
     5401 ggacggcgag tccctggacg tcggcgaccg ccagctccag ctcgtgcgtc cgccggtgta
     5461 cgactcgccg accacccgag gcctgttcga tcccacgacg ggcgtgtact gggccgccga
     5521 cggcttcgcc accccgatgc cggtcccggt gcgcgacgtc gcggacatcg acctcgaggt
     5581 ctggaccgcc ggcatggcga cgttcaacca gtacatcagc ccgtggctgc tgctcgcgga
     5641 cgaacgtcgc taccagggca cggtcgaccg gatcgccgcc ctgcaaccga cggcgatcgc
     5701 cggctgccac accccggtga tcgtggggga ccgcgtggcc ggcgccatcg acatcacccg
     5761 gcgcaccccg ggcgagaccg tgcccgccca acccgaccag gccgtgctgg accagatcca
     5821 gcgcaccctg gtcgaggcag cctgagccca cctggaaccc gggtgcgatc cctcagaccc
     5881 ggggtcgcac ccgcatcggt gcgccgccgg cgggacggtt caccaccatg cccaccggac
     5941 ggggctccac cggggccgtc ggctcgacat cgagccgctg ggccaggcgt gcggcgatca
     6001 gcgtcagctc catctgcgcc agcgcgaagc cgatgcagtt ccgggcgccc ttgccgaagg
     6061 gcacccaggc ggcgtcggcc agcgcctggc gctcggggga gaggtcgagg aagcgatcgg
     6121 gatcgaaccg gagcgggtcg tcccacgccg agggatcgcg cccggcgagg tgggcggacc
     6181 agagcaccag cgtcccggcc gggatctcgt gcccgccgag cacgaggtcg acggcggcct
     6241 ggcggggcga caccacgccg gcggggtgga gccgggtcgt ctcccgcacc acgcgggagg
     6301 ccaggtcgag cgcccggagc gtggacgcgt cgggcgccgg tcccgatccg acgggtccga
     6361 ggacatcgtc ggcctcggct cgcaggcgat cccacacgcc gtcggcgagg gtcgtgcacc
     6421 acagcaacca ggccagcgac gccgacgtcg tgtcatagcc ggcgccgatc agggtcacca
     6481 cctgatcccg gatctcggag tcgtcgagcg tgccgtcgac gacgagggcc tcgagcacgt
     6541 ccagccggtc gccgctcggt cgggcccgtc gctcggcgat ctccgcgtcg atgatccggt
     6601 cgagcgcctt gcggtcggcc cgcacccggg cgcgcgtcgt ccccgggatc gggtggggga
     6661 cctgccggat ggcgggggcc tccaggtagt cctggggccc ctggaacagc gcaccgatct
     6721 cggcggtgcg gtgcgccatc cgctccccga acaggatgcg caccacgagg tccagcacca
     6781 gctcgcgccc ggtcacgtag aggtcacgga cgtggtcgcc gtcggccacc acccggtcga
     6841 tggcagcgtc ggtgcgttcg aggatcagcg ggatccagcc gtcgagccgg cggcggctga
     6901 acgccgcctg caccgagcga cgtcggcgct tgtggtcgtc cccgtcggac acgatcatcg
     6961 agccgggtcc gaccacgaac gccagcacat tgaacgggtg gttccagcgg aacgagtccg
     7021 tggaggtcgc gaacagctcc ttcagggcgg cgggatcgcc gacgatggcc atccgcaccg
     7081 ggccggcgcc gaggcccacg acaggcccgt agcgctcccg gagctcgtcc aggaccggtt
     7141 gggggtcctg gaagatgcga cgcaggtagg cgggctgggc gcgccacggc gggtgcggga
     7201 gcgggcgggg ggaggtggtg gtcggctgca cggtggctcc tccggtcggt ccgccgtcga
     7261 gccatcatcg caggccgtcg tcgatcaggt gcgccggatc agcgccgcgg cgtccttcca
     7321 cgagatgcgc gtcccccggc gcagcagcac ctgctcgtag atccgcccgg tcgcggccca
     7381 cgcggcgagg atcgccagta ccaggagcac gagggcgacc ccgacctcga gcaccgacgc
     7441 cgcgccggcg gccatgcgcg tcggcatgag cagcggcgcg atcggtggga gcatcgacat
     7501 caccgtgctg cccgtcgact ccggatcgga gccgaaggtg aagaccatga cgtaggccag
     7561 gagcaggacg ctcatgatcg gcatcgccgc cgcctgcgcg tcctcctggc ggctcaccag
     7621 cgatccggcg agggcgaaca gcgtggagta gagggcgtag ccgccgagga accacacgag
     7681 ggtcagcggg agcgccgacc agacctcgcc ggggacctcg acgccggaga tggccagcga
     7741 caccagggcg gcgacgatgg cgagcgcgaa ctggagcagc gcggccatgc cgatgccgat
     7801 caccttgccc gccagcatct cgctgccccg ggcccgcacc agcagcacct cgaccacggc
     7861 cgtggccttc tcctcgacga cgcccgtgag cacgtaggtc ccgaacgtct gcaacgagat
     7921 gaacagcagg atgccggcca gcgatccggt gaggagagcg accccctcgt tgccgtcgtc
     7981 gtctccgtcg agcgtcaccg ggtccagctg cgtggtggtc agggcctcgg tgacctccgc
     8041 ctcgtcgagc ccggcgtcgg cgagggcctc ctgcacctgg gcgctggacc acgactgctg
     8101 gaggatggcg aggacctgtt gggcgatgtc ctcgtcgaac acgatctcgc cccggtcgcc
     8161 gatcaccgcg acgtcggcct caccctccgc caccgcggtg cgtgccgcgt cggcgtcgtc
     8221 gaaggccgtg atctcgaccg tgcgctcgaa cagcgcgccg gtctggccgg tcatctcctc
     8281 gaacccggtg ggctgggcac ccgcgaccgc gacgtcgatg gcgtcggcac cgtcgtcgct
     8341 gttgagccgg ctgatgatgc cgatggcgag gatgaccacg acgaggagcc cggtggcgac
     8401 ggcgaacgac cgggctcgca ggcgctcgac cacctctcgc cgggcaacga ggcgcaccac
     8461 gctggtggcg ccgaccggtc gaccgtcgct caccccgtca cctcccggcg ctcgtgggtg
     8521 gggtcctcca ccgtgtcgcg ccccacggcg ccgaggaaca cctccgacag gtcgggtggc
     8581 aggaacgagt agccgaccac cggtgcgagc gagcgggcgt ctgccagcag ccggtcggca
     8641 tccgtgccgg cctccacggc gacgcgatgt cgccggccgc cggcggtgac ctcgctgtcc
     8701 cgaccggcat cccaccgcac ggcctcttcg aactcgacct ccacgtaccg ggtcgggctg
     8761 gcggcccgga gtgcggcgac ctcaccgcgc agcacgatcc ggccgcggtc gacgatgacg
     8821 acggcgctgc acacgtcggc cacgaggtcg agctggtggc tgctgagcag gagcgccgcg
     8881 ccgcggtcga cctgctcccg caggatgtcg ctgagcatct cgaccgcgac cgggtcgaga
     8941 ccggagaacg gctcgtccag cacgagcagg tcggggtcgt tgagcagggc gagggcgagc
     9001 tgcacgcgct gctggttccc gctggagagg ctctggatgg tgtcgtcggc gcggtcgtcg
     9061 aggccgagcc gctcgagcca acggtcggcg gcttccccgg cacggcccga ggtgagaccc
     9121 gacagctccc cgtagtacga gaggtggtcg cgcagcttca tgcgcgggta catcccgcgc
     9181 tccgccggca tgtacccgaa gtgctgccgg acgtcggggt cgacggggac gccgtcccac
     9241 gtgacgctgc cggcatcgag gtggatgagg cgcatgatcg cccgcatggt ggtggtcttg
     9301 cccgagccgt tcggcccgag gaaccccacg atctggcctc gcggcacgtc gaagtcgacg
     9361 tcgtcgagcg ccagcagcgg cccgtagcgc ttcgtcagac cccgcacgca cagcacggcg
     9421 ggcgaccgta gcggtcgggg atcgggttcc cggtcgggcg gcgcgccggt tcagccgggt
     9481 cggggcagga ggcggtggat gccggcgacg gtgagcgcca cgagctcgcc gtcctcgagc
     9541 tcgccgaagg aggtgacgct gctgatccgg tcgatcagct cgaccggttc ggccccgggg
     9601 tcgtcggagg ggacggcgcg gatccaggcc tggcagaagt cgccgaacag gtaccagccg
     9661 tgcaggccgg ggttctcgct cccgcggtag acgtagccgc ccgtgacggc gcatccgtcg
     9721 tcgcggccgt aggtgagcgc cggctcgatg aacgcgtcgg atcgctcccc gtcctcgaag
     9781 ggcagcgtgc cctcgacctc gctccagccg aagtcggggc gggcctcgtc gggatcgacg
     9841 accgagacct cctccagcac ccactggccg acgtcggcca cccacatccg gttcgtgtgg
     9901 cgatcgaagc tccaccgcca cgggttgcgc agcccgatcc cccagatctc ggggcgctgc
     9961 cagtccgggt tcgacccgac cgtgcggatg ccgccatcag ggtcgggcac gacgcgcagc
    10021 atcttgccga gcagcgaccg ctcgtcccgg gcgaggccgt cgggatcgcc cacgccaccg
    10081 ccgtccccgg tggcgatcca gagggcaccg tcgggtccga acacgaggtg gccgccgttg
    10141 tgggtgtcgg tcggctgctc gatctcgagg tgcaggacgc cgtccccgtc ggcgatcggc
    10201 atgccgtcgt cgtcgagcgg ccacgaccgg atctcggtgt cgtcgtcgag cgcggtgtag
    10261 tcgaggtaga cgcggtcgcc gtccgggtcg atcgccatgc cgagcagccc ttgctcgcca
    10321 cccgagctga tccgctcgga gaggtccatg accacctcgg tggtgccggc gtcgaggtcg
    10381 acggccagca cctcgccgcg cagggtcgac acgaggaccg gacccggacc cggcggatcg
    10441 gccagcgccg aggccggcgt ggtctcgatg aggggcccga gcgcgagcgc aggcgcatcg
    10501 cccaccggct cctccggtgc ccgtggcgcc ggcagcggga ggtcgacgct tggcgcggcg
    10561 ttcgacgacg cgccgcacgc ggtggcgagc agcgcggccg cgatcgtcgc gacgatggcg
    10621 agccgggggt ggggcaccag gtcatcttgg acgaactgtg gccggccgct gagtaccgtg
    10681 cgccgccatg gagatcagac tcgacggaca ggtcgcgctc gtgacggggg cgtccaaggg
    10741 gatcggcaag gccatcgcgc agacgctggc cgaggcaggc gccaaggtgg tgctcagctc
    10801 ccgcaagcag gacgccctcg aggcggccgc cgcggacatc gacggggaga cgtcggtctt
    10861 cgccgccaac gccggcgatc ccgacgctgc tgccgcgtgc gtggcccaca ccatggagac
    10921 cttcggccgg gtcgacatcc tcgtcaacaa cgcggccacg aacccgtaca tgggccgccc
    10981 gatcgagatc gacctgcccc gctacgacaa gacctgggag gtcaacctgc gcggcccgct
    11041 cgtgtgggcc caggagtgct ggaaggcgtc catgcaggac aacggcggcg ccgtcatc
//
LOCUS       DATISU010000055        11152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_887649, whole genome shotgun sequence.
ACCESSION   DATISU010000055 DATISU010000000
VERSION     DATISU010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 11152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11152
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887649"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..518)
                     /locus_tag="VLR27_02450"
     CDS             complement(3..518)
                     /locus_tag="VLR27_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HSP02331.1"
                     /translation="MPDSESAPTAESDEEVEHGELIDAAADGADGAEQQEAVQEPGRV
                     MRIGAMIRQLLEEVRSADLDEAGRDRLREIYDQSVQELSGALSDDLAEELHRLASPFG
                     DTPASAGELRVAQAQLVGWLEGLFHGIQATLFAQQAAARQQLEQMRHQLESGQRPGNE
                     GGPGHPPGTYL"
     gene            complement(561..1664)
                     /locus_tag="VLR27_02455"
     CDS             complement(561..1664)
                     /locus_tag="VLR27_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HSP02332.1"
                     /translation="MADVPLIPDYAGACVCNVAAVVQDPPDELADWVPRALAGAEQST
                     VLLTLDGLGWNQLQDRRSIAPTLTSVLQGGPVSTVAPSTTSTALTSIVSGMPPGEHGV
                     IGYRMSIDGHVLNVLRWATADGDARRSMPPKEFQLHEPFGGQRPPVVTKAEFSETGFT
                     DAHLGGTRFVGYRTMGTLVTEINRLVRSGEPFVYAYYEGIDKVAHEYGLADHYDNELR
                     WVDHLVADLLEVLPAGTVLAITADHGQIEVGERLVELDPGVRPHLSMQSGEGRFRWLH
                     ARPGRTRQLLEAAEAHADTGWVLSRDQMVDEGWFGPRVLDHVRARLGDVALVAREPVA
                     YIDPADTGPYHLIGRHGSLTADEMLVPFLAATV"
     gene            1728..2204
                     /locus_tag="VLR27_02460"
     CDS             1728..2204
                     /locus_tag="VLR27_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSP02333.1"
                     /translation="MRVSTRGDYASRALLSLALDGVDTPVSVRDIATRTGLPQPYLEQ
                     ILLALKGAGLVRSKRGVGGGYVLARSPDDITLGDIVSAVDGPITLGDFGEPHTGGACD
                     HEGQCVLLAVWADVAEHMRRHLDGLHLADLARMANGEQPWPAPPHDDHDEPVSDAV"
     gene            complement(2291..3211)
                     /locus_tag="VLR27_02465"
     CDS             complement(2291..3211)
                     /locus_tag="VLR27_02465"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HSP02334.1"
                     /translation="MTAFDAELPEALAGERLDRIVAMLCDVPRSVAGELIDAGEVRVD
                     GEVRRRRADRLDEGQRLALDVPDAPAELVVEADPDVPVDVVHEDEHLLVIDKPAGLVV
                     HPGAGNPTGTLVHGLLARYPEIADVGEPFRPGIVHRLDRETSGLLAVARTAEAHAGLT
                     ELLRVHDIVRRYRTLVWDHVTEPRGQVDAPIGRSTKDPTKMGIVVGGREARTGYEVVR
                     RYDEPVPCTELVCHLETGRTHQIRVHLRGIGHPVVGDPRYGGARQTLPAPRQFLHAEH
                     LAFRHPVTGEPVEADSPLPADLQAVLARLA"
     gene            complement(3208..3768)
                     /gene="lspA"
                     /locus_tag="VLR27_02470"
     CDS             complement(3208..3768)
                     /gene="lspA"
                     /locus_tag="VLR27_02470"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HSP02335.1"
                     /translation="MGSVGAETTAPSTPGDPAPRPRRPLLVIGAVTAVVVVLDQLTKW
                     WALEALTEPTRVIDLVWTLRLRVIYNTGTAFSLTSDSGPIVSVIALVVVAFLLWSGRN
                     QRSPWVLASYGLVVGGTFGNMVDRVFRDGNDGVLGGAVIDFVDLQWFPVFNLADAALT
                     VGIGLLLVVGFFTDALDDPAEGETGA"
     gene            complement(3749..4417)
                     /locus_tag="VLR27_02475"
     CDS             complement(3749..4417)
                     /locus_tag="VLR27_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HSP02336.1"
                     /translation="MARASKASAASKKKATTKKAAAKKASSGGAAKATTKSTKKAAKK
                     AAPAKKAKATSKSSASKSTSSKSSSTKSSAKSSSAKETTKKSAPAKSGRTKKSSHFDS
                     KFLEKMRKLLHAERDTYERQAKQLRAEADSLVMDMDPGDVQFDEESGEGDTIAVERGR
                     DLALAAQAMDMIEEIDHALTKFDEGTYGICEVSGEPIPEERLEAIPWAREKVEYKVGG
                     FGRR"
     gene            4536..7727
                     /gene="ileS"
                     /locus_tag="VLR27_02480"
     CDS             4536..7727
                     /gene="ileS"
                     /locus_tag="VLR27_02480"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HSP02337.1"
                     /translation="MPANDGRPGSGRYPDVDAHPSFPELEKAIIQRWEAEKTFERSID
                     QREGGDEFVFYDGPPFANGLPHYGHLLTGFVKDAVPRFQTMRGKKVERRFGWDCHGLP
                     AEMEAEKRLGVSGRLAIEELGVDTFNQECRLLVSGTADSWNSYVRRQARWVDMANDYK
                     TMDLSFMESVMWAFKTLHDKDLAYEGQRVLPYCWECETPLSNFETRMDDAYRERQDPA
                     VTVWFELTSGDHAGMRLLVWTTTPWTLPSNLAIAVGPAITYAVYRDADGAEYALGEET
                     VEKYATQLDGMEQVGTVTGADLVGASYTPIFDFFAGTENAHQILAADWVDTGEGTGSV
                     HIAPGFGEDDKALSDQHGIPTVAPVDEKGAFTADVPPYAGMQVFDANKDIVRDLKARG
                     VIVRHDSYLHSYPHCWRTDTPLIYKAMSSWFVDVTAIKDRMLELNQQINWVPEHVRDG
                     SFGRWLEGARDWSISRNRFWGSPIPVWKSDDPTYPRIDVYGSIEDLERDFGVTVTDLH
                     RPMIDELTRPNPDDPTGESTMRRIPDVLDCWFESGSMPFAQVHYPFENQEWFDGHFPG
                     DFICEYIGQTRGWFYTLHVLSTALFDRPAFSNCVVHGVLLGDDGQKLSKRLQNYPDPV
                     EVFDTIGSDAMRWSLLSSAAVRGGDMVAAKGPMQDAVRQVLLPIWNAWYFLALYANAG
                     DSLRAGRTLTADDVADGSVEHVLDRYVLAKTRLLVELVTARMDAFDLSGACGDISSFL
                     DSLNNWYIRRSRERFWGEDQVAVDVLHTVLDHLCRVSAPLLPLLTEQVWGDLTGEGSV
                     HLTDWPDADALPHDPELVVQMDLVRDIASAAKSVREARGLRRRLPLQTLTVSLAAAAG
                     TDNAHLEAFRGLLADEVNVKDVVLADAGTLGAERFEVDLKVVGRRLGKDTPTVVKAMQ
                     SGDYTYDADADELVVGTHRFGPDEFVRKLDADEPEATAALGGGAGLVRLDLTVTPELE
                     AEGLVRDLAREVNEVRRREGLDVSDRIRLVVDPGHHDDLRAAVEAHEGLLTDSCLVAE
                     LEQAPHERPLADGHRVEVGAGLAAHVAVQRIGS"
     gene            complement(7737..9248)
                     /locus_tag="VLR27_02485"
     CDS             complement(7737..9248)
                     /locus_tag="VLR27_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02338.1"
                     /translation="MLVLVSFGLVLLATILLVVGLLADNLTLIYISIASSFTAAVVLY
                     LAFRRARPKADTDLVAPTPIPPEGAEDDFDEPAVTTITPAATTPESVVAAAEEPEAVE
                     PEPQPEPVLAGDPASSAAGDDWTSDDDWADDGEFDVEFPIADYDDLTVAEIMPLLPQL
                     YSDELGVVAERERTGKNRTAILSRLDELATTGTDADALEAVSAADDADEAFAPAAMPA
                     TTPAPEAPRNDALSTPRPAPAAPQVPSLSDDDDDGFVFPIADYDQLSVDQIVPLLAQL
                     EDDELEDVKAREAAGQGRKSVIAEIDRQLGLVDALDTASVPDEGWAEPDPTPAPAPEA
                     RPQPEPETAASSVLAIAGYDGLTVAQIRPLLAGLSHDELRAVLDHETARDNRRTIVAD
                     IERRLGAAEPAAPAPAARPAARTTKAPARKATKKAAAKRTTAKRAAKKAARATKKAAA
                     PRFPIADYDTLTVAQIRPQLAGLSEPELRQVQAREESGAGRKTILNEIASRLG"
     gene            complement(9389..10336)
                     /locus_tag="VLR27_02490"
     CDS             complement(9389..10336)
                     /locus_tag="VLR27_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HSP02339.1"
                     /translation="MDLTPDLLLEAKFAPARRGYDMNEVDDFLERCAEGLDVLLARLR
                     AEFDRAERAEAALVEMRATGAAPATAEAPAAEPVGEAVPEVAPAAAPDPDRVQEPMRI
                     LIAAERTAEAAIADARAEAERIRAEAESVASTTRSEAESMLARSRAEAEAEARKAGDE
                     TRRSIEAEVMALRQDREVLSEDVASLRSWLDSQRDRLRTAAQEMQRIVDDPEALRELP
                     APEVQSDDAGAAVSGADEPVADEPPAAPPAAAHDDEVVDARSAEDVQGRLHGGFFDDD
                     EPTQAVPVAGGPEASGQTAVFDDDRAPTNGAFFGSDAIR"
     gene            complement(10440..10682)
                     /locus_tag="VLR27_02495"
     CDS             complement(10440..10682)
                     /locus_tag="VLR27_02495"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HSP02340.1"
                     /translation="MGLICSLLQLYVIVIIAGALLSWFPTEPGGGLHQVRVAIARVTD
                     PVMLPVRRAIGTSFGGIDFSPLIVIIGLQLLAGIIC"
     gene            complement(10695..>11152)
                     /gene="sepF"
                     /locus_tag="VLR27_02500"
     CDS             complement(10695..>11152)
                     /gene="sepF"
                     /locus_tag="VLR27_02500"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HSP02341.1"
                     /translation="QGPGGPRMPQPPPEPEEPAGAYGRGPSARPSGAVRPLPSRAQAE
                     NPSRAPQREETPTVRPMRPTSAKPTVVAPTSFDAAKDVADRFKANQPVVMDLSDVDRE
                     LARRLIDFSSGMCYALGGNMERVTPGDYLLTPSGVEVSGEERRRLAGGR"
BASE COUNT         1473 a         3944 c         4160 g         1575 t
ORIGIN      
        1 cgctacaggt aggtgcccgg cgggtgcccg gggccgccct cgttgccggg ccgctggccc
       61 gactcgagct ggtggcgcat ctgctcgagc tgctggcgag cggcggcctg ctgggcgaac
      121 agggtggcct ggatcccgtg gaagaggccc tcgagccagc cgaccagctg ggcctgggcg
      181 acccgcagct cgcccgccga ggccggcgtg tcgccgaagg gcgaagcgag gcggtggagc
      241 tcctcggcga ggtcgtcgga gagggccccg gagagctcct gcaccgactg gtcgtagatc
      301 tcccggaggc ggtcgcggcc ggcctcgtcg aggtcggccg agcgcacctc ctcgagcagc
      361 tggcggatca tggccccgat gcgcatcacg cgaccgggct cctgcacggc ctcctgctgc
      421 tcggcaccgt cggctccgtc tgcggccgcg tcgatgagct cgccgtgctc cacctcctcg
      481 tcgctctcgg cggtgggggc gctctcggag tcgggcacgt ggtcctccag gggtcgtcgg
      541 cggtggcggc gcggagccgc tcagacggtg gcggcgagga acgggacgag catctcgtcc
      601 gcggtcagcg aaccgtggcg cccgatgagg tggtacgggc cggtgtcggc ggggtcgatg
      661 taggccacgg gctcacgggc gaccagggcc acgtcaccga ggcgggcccg gacgtggtcg
      721 aggacgcggg gaccgaacca gccctcgtcg accatctggt cgcggctgag cacccagccg
      781 gtgtcggcgt gggcctcggc ggcctcgagc agctggcggg tgcggcccgg gcgggcgtgg
      841 agccagcgga agcgcccctc gccgctctgc atcgacaggt gggggcgcac gccggggtcg
      901 agctcgacga ggcgctcgcc cacctcgatc tggccgtggt cggccgtgat ggcgagcacg
      961 gtgccggcgg gcagcacctc gagcaggtcg gcgacgaggt ggtcgaccca gcgcagctcg
     1021 ttgtcgtagt ggtcggcgag gccgtactcg tgggcgacct tgtcgatgcc ctcgtagtac
     1081 gcgtagacga agggctcccc gctgcggacc aggcggttga tctcggtgac gagcgtcccc
     1141 atcgtgcggt agcccacgaa gcgggtgccg ccgaggtggg cgtcggtgaa gccggtctcg
     1201 gagaactcgg ccttggtgac caccggggga cgctggccgc cgaagggctc gtgcagctgg
     1261 aactccttgg ggggcatcga ccggcgggcg tcgccgtcgg ccgtggccca gcggagcacg
     1321 ttgaggacgt ggccgtcgat cgacatgcgg tacccgatga cgccgtgctc gccgggcggc
     1381 atgccgctga cgatggaggt cagcgcggtg gacgtggtcg acggggcgac cgtggacacc
     1441 gggcccccct gcagcaccga cgtgagggtg ggcgcgatcg agcggcggtc ctgcagctgg
     1501 ttccagccca gcccgtcgag ggtgagcagc acggtggact gctcggcgcc cgcgagggcc
     1561 cggggcaccc agtcggcgag ctcgtcgggg gggtcctgga ccaccgccgc cacgttgcac
     1621 acgcaggcac cggcgtagtc agggatgagc ggtacgtctg ccactcctgg ccacgctagc
     1681 gaccgccacc gcggacaccg ccaccagggg tgcctacgat gccagccatg cgcgtgtcga
     1741 ccagagggga ctacgccagc agggcgctcc tgtccctcgc cctcgacggc gtcgacaccc
     1801 cggtctcggt ccgcgacatc gccacccgca ccggattgcc ccagccctac ctcgagcaga
     1861 tcctgctcgc cctgaagggc gccgggctcg tgcgctccaa gcgcggcgtc ggcggcggat
     1921 acgtgctggc ccgctccccc gacgacatca ccctcggcga catcgtgtcc gcggtcgacg
     1981 gccccatcac cctgggcgac ttcggcgagc cccacaccgg cggggcgtgc gaccacgagg
     2041 gccagtgcgt gctgctggcg gtgtgggccg acgtggccga gcacatgcgc cgccacctcg
     2101 acgggctcca cctcgccgac ctggcccgca tggccaacgg cgagcagccc tggcccgcgc
     2161 caccccacga cgaccacgac gagccggtca gcgacgccgt ctgagcctgg acgagcgcgc
     2221 cgatcggttc gttggtggcg ccctgcggca cgaacgcgcc gatcggtccg ttcgtacctt
     2281 cgccgccggg tcaggcgagg cgggcgagga cggcctggag gtcggcgggc agcgggctgt
     2341 cggcctcgac cggctcgccc gtgaccgggt ggcggaaggc gaggtgctcg gcgtggagga
     2401 actggcgcgg ggcgggcagc gtctggcggg cgccgccgta gcgggggtcg cccacgaccg
     2461 ggtggccgat gccgcgcagg tggacgcgga tctggtgggt gcgaccggtc tcgaggtggc
     2521 agaccagctc ggtgcagggc acgggctcgt cgtagcggcg cacgacctcg tagccggtgc
     2581 gggcctcccg gcccccgacg acgatgccca tcttggtcgg gtccttggtc gagcgcccga
     2641 tgggggcgtc gacctggccg cggggctcgg tgacgtggtc ccagaccagg gtccggtagc
     2701 gacgcacgat gtcgtgcacc cggagcagct cggtgagccc ggcgtgggcc tcggccgtgc
     2761 gcgccaccgc gagcagcccg gaggtctccc ggtcgaggcg gtgcacgatg ccgggccgga
     2821 acggctcgcc gacgtcggcg atctcggggt accgggcgag gaggccgtgg acgagcgtgc
     2881 cggtggggtt gccggcaccg gggtggacca cgagcccggc cggcttgtcg atgaccagga
     2941 ggtgctcgtc ctcgtggacc acgtcgaccg ggacgtcggg gtcggcctcg acgacgagct
     3001 cggccggggc gtcgggcacg tcgagtgcga ggcgctgtcc ctcgtccagt cggtcggctc
     3061 ggcgccggcg gacctcgccg tcgacccgga cctcgccggc gtcgatcagc tcgccggcga
     3121 cgctgcgggg gacgtcgcac agcatggcca cgatccggtc cagccgctcg ccggccaggg
     3181 cctcgggcag ctcggcgtcg aaggcggtca tgccccagtc tcgccctcag cggggtcgtc
     3241 cagcgcatcg gtgaagaacc cgaccaccag gagcaggccg atcccgacgg tgagggcggc
     3301 gtcggcgagg ttgaagaccg ggaaccactg gaggtcgacg aagtcgatga ccgctccgcc
     3361 gaggacaccg tcgttgccat cccggaagac ccggtcgacc atgttgccga aggtcccgcc
     3421 gacgacgagg ccgtaggagg cgagcaccca cggggagcgc tggttgcgac ccgaccagag
     3481 caggaaggcg accacgacca gggcgatcac cgacacgatc gggccgctgt cggaggtgag
     3541 cgagaacgcg gtgccggtgt tgtagatgac ccgcaggcgc agggtccaca ccaggtcgat
     3601 gacccgggtg ggctcggtga gggcctcgag cgcccaccac ttggtcagct ggtcgaggac
     3661 gacgacgacc gcggtgaccg ccccgatgac caggagcggg cgccggggcc ggggggcggg
     3721 gtcgccgggc gtcgaggggg ccgtcgtctc agcgccgacc gaacccaccg accttgtact
     3781 ccaccttctc ccgagcccac gggatcgcct cgaggcgctc ctccgggatc ggctcgcccg
     3841 acacctcgca gatgccgtag gtgccctcgt cgaacttcgt cagggcgtgg tcgatctcct
     3901 cgatcatgtc catcgcctgg gccgcgaggg ccaggtcgcg gcctcgctcg accgcgatcg
     3961 tgtcgccctc gccggactcc tcgtcgaact ggacgtcgcc cggatccatg tccatgacca
     4021 ggctgtcggc ctcggcgcgc agctgcttgg cctggcgctc gtaggtgtcg cgctcggcgt
     4081 ggaggagctt gcgcatcttc tcgaggaact tggagtcgaa gtgcgacgac ttcttggtgc
     4141 gaccggactt cgccggcgcc gacttcttcg tcgtctcctt ggccgaggac gacttcgccg
     4201 acgacttggt cgacgaggac ttcgacgacg tcgacttcga ggcagagctc ttcgaggtgg
     4261 ccttggcctt cttggccggg gcggccttct tggctgcctt cttcgtcgac ttggtcgtgg
     4321 ccttcgcggc cccgccggag ctggccttct tggccgccgc cttcttcgtc gtcgccttct
     4381 tcttgctggc cgcagaggcc ttgctggcac gtgccacgtg gtgctcctcc gcacagggga
     4441 gccgagagcg tacgccgcgc cccgcgcggc agcaaggacc cccacggtcg ggcaaccctt
     4501 ggggagccgt gaccgggcgc ccctaccatg gcgggatgcc tgcgaacgac ggacgcccag
     4561 ggtcgggtcg ctaccccgac gtcgacgccc acccctcgtt cccggagctc gagaaggcga
     4621 tcatccagcg ctgggaggcg gagaagacct tcgagcgcag catcgaccag cgcgagggcg
     4681 gcgacgagtt cgtcttctac gacgggccgc cgttcgccaa cggcctcccc cactacgggc
     4741 acctgctgac cggcttcgtc aaggacgcgg tcccccgctt ccagacgatg cggggcaaga
     4801 aggtcgagcg ccgcttcggc tgggactgcc acggcctgcc cgccgagatg gaggccgaga
     4861 agcgcctcgg cgtgtccggc cgcctcgcca tcgaggagct cggcgtcgac accttcaacc
     4921 aggagtgccg cctgctcgtg tcgggcaccg cggactcgtg gaactcctac gtccgccgcc
     4981 aggcccgctg ggtcgacatg gccaacgact acaagaccat ggacctgtcg ttcatggagt
     5041 cggtcatgtg ggcgttcaag acgctgcacg acaaggacct ggcctacgag ggccagcggg
     5101 tcctgccgta ctgctgggag tgcgagaccc cgctctcgaa cttcgagacc cgcatggacg
     5161 acgcctaccg ggagcgccag gacccggccg tcaccgtctg gttcgagctg acctcgggcg
     5221 accacgcggg catgcgcctg ctggtgtgga cgacgacgcc gtggaccctg ccgtcgaacc
     5281 tggccatcgc cgtcggcccg gccatcacct acgccgtgta ccgggacgcc gacggcgccg
     5341 agtacgcgct gggcgaggag acggtcgaga agtacgccac ccagctcgac gggatggagc
     5401 aggtcggcac ggtcaccggc gccgacctcg tgggtgcctc ctacaccccg atcttcgact
     5461 tcttcgccgg cacggagaac gcccaccaga tcctcgccgc cgactgggtc gacaccggcg
     5521 agggcaccgg gtcggtccac atcgcccccg gcttcggcga ggacgacaag gcgctgtccg
     5581 accagcacgg catccccacc gtcgcgccgg tcgacgagaa gggcgccttc accgccgacg
     5641 tgccgccgta cgccggcatg caggtcttcg acgccaacaa ggacatcgtc cgcgacctga
     5701 aggcgcgcgg cgtcatcgtc cgccacgact cctacctgca cagctacccc cactgctggc
     5761 gcaccgacac gcccctgatc tacaaggcca tgtcgtcctg gttcgtcgac gtcaccgcga
     5821 tcaaggaccg gatgctggag ctgaaccagc agatcaactg ggtgcccgag cacgtccgcg
     5881 acggctcctt cggccgctgg ctggagggcg cccgcgactg gtcgatctcc cggaaccggt
     5941 tctggggctc gcccatcccg gtgtggaagt ccgacgaccc cacctacccg cgcatcgacg
     6001 tctacggctc catcgaggac ctcgagcgcg acttcggcgt caccgtcacc gacctgcacc
     6061 ggccgatgat cgacgagctc acgcgcccca atcccgacga ccccaccggc gagtcgacca
     6121 tgcggcgcat ccccgacgtc ctcgactgct ggttcgagtc gggctcgatg cccttcgccc
     6181 aggtgcacta ccccttcgag aaccaggagt ggttcgacgg ccacttcccc ggggacttca
     6241 tctgcgagta catcggccag acccgcggct ggttctacac gttgcacgtg ctctcgacgg
     6301 cgctgttcga ccgcccggcg ttctcgaact gcgtggtcca cggcgtgctg ctcggcgacg
     6361 acggccagaa gctgtccaag cggctgcaga actaccccga tcccgtcgag gtgttcgaca
     6421 ccatcggctc cgacgccatg cggtggtcgc tgctcagctc ggcggccgtg cgcggcggcg
     6481 acatggtcgc ggccaagggt ccgatgcagg acgccgtgcg ccaggtgctg ctgccgatct
     6541 ggaacgcctg gtacttcctc gccctctacg ccaacgccgg cgactcgctg cgggccgggc
     6601 gcaccctcac cgccgacgac gtggccgacg gctcggtcga gcacgtgctc gaccgctacg
     6661 tgctggccaa gacccgcttg ctggtggagt tggtcaccgc ccgcatggac gcgttcgacc
     6721 tgtccggcgc gtgtggcgac atctcctcgt tcctggactc gctgaacaac tggtacatcc
     6781 gtcgctcacg ggagcggttc tggggcgagg accaggtggc cgtcgacgtg ctccacacgg
     6841 tgctcgacca cctgtgccgg gtctcggctc ccctgctgcc cctgctcacc gagcaggtct
     6901 ggggcgacct caccggcgag ggttcggtgc acctcaccga ctggcccgac gccgacgcgc
     6961 tgccccacga ccccgagctg gtcgtccaga tggacctcgt gcgcgacatc gcctcggccg
     7021 ccaagtcggt gcgcgaggcg cgcggcctgc gccgccgcct gccgctgcag accctcaccg
     7081 tctccctcgc cgccgcggcc ggcaccgaca acgcccatct cgaggccttc cgcggcctgc
     7141 tcgccgacga ggtcaacgtc aaagacgtcg tgctcgccga cgccgggacc ctcggcgccg
     7201 agcgcttcga ggtcgatctc aaggtcgtgg gccgccgcct cggcaaggac acgccgacgg
     7261 tcgtcaaggc gatgcagtcc ggcgactaca cctacgacgc cgacgccgac gagctcgtgg
     7321 tcggcacgca ccgcttcgga cccgacgagt tcgtgcgcaa gctcgacgcc gacgagcccg
     7381 aggccaccgc cgccctcggg ggcggcgcgg ggctggtccg cctcgacctg accgtcacgc
     7441 ccgagctcga ggccgagggc ctggtgcgcg acctcgcccg cgaggtcaac gaggtccgcc
     7501 gcagggaggg cctcgacgtg tccgatcgca tccgcctggt cgtcgacccc ggccaccacg
     7561 acgacctgcg ggccgccgtc gaggcccacg aggggctgct caccgactcc tgcctggtcg
     7621 ccgagctcga gcaggcgccc cacgagcgcc cgctggccga cggccaccgc gtcgaggtcg
     7681 gcgccggcct ggccgcccac gtggcggtcc agcgcatcgg ctcctgacgg gcctcgtcag
     7741 ccgaggcggc tggcgatctc gttcaggatc gtcttgcgtc cggcgccgga ctcctcccgg
     7801 gcctgcacct ggcgcagctc gggctcgctg aggccggcca gctggggacg gatctgggcg
     7861 acggtgaggg tgtcgtagtc cgcgatcggg aaccggggtg cggccgcctt cttcgtggcc
     7921 cgggccgcct tcttggcggc ccgctttgcg gtggtgcgct tggcggcggc cttcttggtc
     7981 gccttccggg ccggggcctt ggtggtccgg gctgccggcc gggctgccgg ggccggggcg
     8041 gcgggctcgg cggcgccgag gcggcgctcg atgtcggcca cgatcgtgcg gcggttgtcg
     8101 cgggcggtct cgtggtcgag cacggcgcgc agctcgtcgt gggacagccc ggcgagcagc
     8161 ggtcggatct gggcgacggt gaggccgtcg taaccggcga tggccagcac gctggaggcg
     8221 gccgtctccg gctcgggctg cggacgggcc tcgggagccg gcgcgggggt ggggtcgggc
     8281 tcggcccagc cctcgtcggg caccgaggcg gtgtcgaggg cgtcgacgag gccgagctgg
     8341 cggtcgatct cggcgatgac gctcttgcgc ccctgaccgg cggcctcgcg ggccttgacg
     8401 tcctcgagct cgtcgtcctc gagctgggcg agcagcggca cgatctggtc gacgctcagc
     8461 tggtcgtagt cggcgatcgg gaagacgaag ccgtcgtcat cgtcgtcgga cagcgacggc
     8521 acctggggtg cggccggcgc cggccgcggg gtcgacaggg cgtcgttgcg gggcgcctcc
     8581 ggtgcggggg tggtggccgg catggcagcc ggggcgaagg cctcgtcggc gtcgtcggcc
     8641 gcgctcacgg cctcgagggc atcggcgtcg gtgccggtgg tggccagctc gtccaggcgg
     8701 gagaggatgg cggtcctgtt cttcccggtg cgctcccgct cggccaccac gccgagctcg
     8761 tcggagtaca gctggggcag cagcggcatg atctcggcga cggtcaggtc gtcgtagtcg
     8821 gcgatgggga actcgacgtc gaactccccg tcgtcggccc agtcgtcgtc ggacgtccag
     8881 tcgtcaccgg ccgcgctcga ggcggggtcg ccggcgagga cgggctcggg ctggggctcc
     8941 ggctcgacgg cctcgggctc ctcggcggcg gcgaccacgg actcgggggt ggtggccgcc
     9001 ggcgtgatcg tggtgacggc gggctcgtcg aagtcgtcct cggccccctc cggcgggatg
     9061 ggggtgggcg ccaccaggtc ggtgtcggcc ttgggacggg cccggcggaa cgccaggtac
     9121 agcaccacgg cagcggtgaa gctggaggcg atggagatgt agatgagcgt gaggttgtcc
     9181 gcgagcaacc ccaccaccag caggatcgtc gccaacaaga cgaggccgaa gctcacgagg
     9241 accagcacgt tcacatcacc ttccgagagc ccgcacggcg gcggggatca tagatcgcgg
     9301 cccgcgcgag ggagcgaagc gggcacaagg gaactctttc gacatccggc gccgaacccc
     9361 tgcccagccg ggccggacgg tcgggcagct accggatggc gtcggaaccg aagaacgcgc
     9421 cgttcgtcgg tgcccggtcg tcgtcgaaca ccgcggtctg gccgctggcc tcgggaccac
     9481 cggccaccgg caccgcctgg gtcggctcgt cgtcgtcgaa gaacccccca tgcaggcgac
     9541 cctgcacgtc ctcggcggag cgggcatcga cgacctcgtc gtcgtgggcc gctgcgggcg
     9601 gcgctgccgg gggctcgtcg gcgaccggct cgtcggcacc ggacacggcg gcaccagcgt
     9661 cgtcgctctg gacctcgggg gccgggagct cgcgcagggc ctcgggatcg tcgacgatcc
     9721 gctgcatctc ctgggccgcc gtgcggaggc ggtcgcgctg gctgtcgagc cagctgcgca
     9781 gcgacgcgac gtcctcggag aggacctctc ggtcctggcg cagggccatg acctcggcct
     9841 cgatggagcg gcgggtctcg tcgccggcct tgcgggcctc ggcctcggcc tcggcgcggg
     9901 agcgggccaa catgctctcg gcctcgctgc gggtggtgct ggccacggac tcggcctcgg
     9961 cgcggatgcg ctccgcctcg gcgcgcgcat cggcgatggc ggcctcggcg gtgcgctcgg
    10021 ccgcgatgag gatccgcatc ggctcctgca cgcggtccgg gtcgggggcg gcggcgggcg
    10081 ccacctccgg cacggcctcg ccgacgggct cggccgcggg tgcctcggcg gtggccgggg
    10141 ccgcgccggt ggcgcgcatc tcgaccaggg cggcctcggc ccgctcggcg cggtcgaact
    10201 cggcccggag gcgggcgagg aggacgtcga ggccctcggc gcagcgctcg aggaagtcgt
    10261 cgacctcgtt catgtcgtaa ccccgacggg cgggagcgaa cttggcctcg aggagcagat
    10321 caggcgtcag atccatgccc acgatgctag gcaccgccgc cgtcaggacg gggtgatggt
    10381 ggcgcgacgg gagcgcgtcc aggacagatc cgggttcccg acccgcccac gaccccggat
    10441 cagcagatga tgccggcgag cagctgcagc ccgatgatca cgatcagcgg ggagaagtcg
    10501 atgccgccga agctggtgcc gatggcccgg cggacgggca gcatcaccgg gtcggtgacc
    10561 cgggcgatgg ccacccggac ctggtgcagg ccgccaccgg gctcggtggg gaaccacgac
    10621 agcagcgcgc ccgcgatgat cacgatcacg tacagctgga gcagcgagca gatcaggccc
    10681 acgacgggcc tcgctcagcg cccgccggcg agccggcggc gctcctcgcc ggacacctcg
    10741 acccccgagg gggtcagcag gtagtcgccc ggggtcacgc gctccatgtt gccgccgagg
    10801 gcgtagcaca tgccggagga gaagtcgatg aggcgacggg cgagctcgcg gtcgacgtcg
    10861 gagaggtcca tcaccacggg ttggttggcc ttgaagcggt cggccacgtc cttggcggcg
    10921 tcgaaggacg tcggcgcgac cacggtgggc ttggccgagg tgggtcgcat gggtcgcacg
    10981 gtgggggtct cctcgcgctg gggggcgcgt gatgggttct cggcctgcgc acgggacggc
    11041 agcgggcgca ccgcgccgct cggccgggcg ctcgggccgc ggccgtaggc gccggcgggc
    11101 tcctcgggct cgggcggggg ctggggcatg cgggggccgc cggggccctg tt
//
LOCUS       DATISU010000056        13395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_2817, whole genome shotgun sequence.
ACCESSION   DATISU010000056 DATISU010000000
VERSION     DATISU010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 13395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13395
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2817"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VLR27_02505"
     CDS             <1..1164
                     /locus_tag="VLR27_02505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HSP02342.1"
                     /translation="DLMLPAFDEIRADFDLGAGSTAVTGLVTAYFVGLGLGTVVYGPL
                     ADHKGRRWTMRLGLGIYIAGALLAAVAPTLPMVMLARFLWGMGAAGPRVVALAIVRDR
                     FEGDAMSRTMSSLMAVFLLVPVLAPTLGALLLEVAPWRWLFVLCAVGALLVALWTQRL
                     PETLAAEHRIPDLRFHRVKTATRLVLSSRTAVAYAAATVALYGVFASYLGSSEAIIGQ
                     ALDSEAQFPLIFGALAGSMGVAAVLNGRIVSRFGTRSMVRGALSVYVVMTVVLLAVAV
                     VTAGQPALWVFMPVMALLLASHAQLLPNLNTLAMAPMAAVAGTASSVIGAAQLTLGAV
                     LGGVLDRFFDGTILPLSIGFALASVLTVAAVTVARRVPAEDVDLVVAGAEPST"
     gene            1233..2858
                     /locus_tag="VLR27_02510"
     CDS             1233..2858
                     /locus_tag="VLR27_02510"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP02343.1"
                     /translation="MDFHFASAWERVADTVPDVEAVVMGDQRWTYAAFDDAAARFASA
                     MEAAGVSRGSQVSLYLHNCPEYLIAQHAAFKHGCGPVNVNYRYLDDELVYLVDNSDSE
                     VLVFHASLGDRVGRVRDRLDRVRLFVVVDDDGTAAEHDFASGFHDLVAAHEPQARWDR
                     SPQDLYMLYTGGTTGLPKGVMYDNGVFCNALLAGAALLNGLPAPASLDDVGDLVRGVR
                     ELGPVVSVPCCPLMHGTGMWVGAMPALNVGGTVVLLQSRSFDADELLTVIEAEGVTRC
                     AIVGDAFARPILRAMEAADARGELPDLSSLKTISSSGAMWSAEIKDGLTRFTSAVLSD
                     GLGSTEGGGYGTVTADRKAKGTTARFTPTPDTLVLGPDDLPVTPGSEEPGLLATRTGA
                     HGYYKDPEKTARTFKQIDGNGYVITGDWATLHEDGTITLHGRGSNCINSGGEKIFPEE
                     VEEALKTHAGVDDCYVVGIPDARFGQRVVAVVGCAAGAEPPAEDDLRSHLREVLASYK
                     IPRQIVVVDHLHRAPNGKADYGWAREQVLATHA"
     gene            2919..4268
                     /locus_tag="VLR27_02515"
     CDS             2919..4268
                     /locus_tag="VLR27_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HSP02344.1"
                     /translation="MPTRRANWRTLRHPAQYVAVAFLGASLLGGLLLWLPFATEPGES
                     TSFVTALFTATSAVCVTGLNVVDPASHWSTFGELVILGLIQIGGFGIIALSSLLVAVL
                     ARRLGLRHRLIAVAETGALDMSDVRRLLVNVAKLTLVVEVVGAVLLAGRFAATHGESV
                     PRAAYLGVFHSISAFNNAGFALFADSLVGYQRDSYILVVIAVAIIIGGLGLPVWSQLG
                     RHRFRARSWSLHAKLTVTTVVALILGGWGLFAWFEWTNPDTLGQLSAWDSTINAFFHS
                     VTPRTAGFNSVDIASLREPSRLLTEVLMFIGGGSASTAGGIKVTTFAVLGWIMWAEAR
                     GDPDVVVFERRVPQAAQRQALTVALLAIGVVIGSTMLLLATSPLGRADLMFEVISALA
                     TVGLSANITPLLSVTSQVVIIALMIVGRVGPITLFAALVLREHDRLYRHPEERPIIG"
     gene            4261..4962
                     /locus_tag="VLR27_02520"
     CDS             4261..4962
                     /locus_tag="VLR27_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HSP02345.1"
                     /translation="MADHPRPRRNRPALQRSKSSQVLVVGLGRFGTAVALELTQLGFE
                     VLGVDTSARRVQKLADDLTHVLEADTTDPEVLTQIGARDFENAVVGIGSDIEASILTT
                     AALADLGVPNIIAKAVTTPHGRILERVGADSVVYPEHEMGRRVAHMVGGSILDWFQLD
                     ANFAMVETHAPAETLGRTLAELELRARYGVTVVCVKPGEEGFTYATADTVLEEGDVLV
                     VAGPGKECEAFARMS"
     gene            complement(4985..5914)
                     /locus_tag="VLR27_02525"
     CDS             complement(4985..5914)
                     /locus_tag="VLR27_02525"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSP02346.1"
                     /translation="MPAFPDLEIRHLAALVAVAEEGSFARAATALGFTQSAVSQQIAS
                     LERAVGLPVLDRPKGPRPAELTPAGRLLLDHARDVLDRIEAVSAELDQLRRGVTGRLV
                     IGTFQSVSAEILPPIVGRMRAEVPQVDIGLFETDDQTALVERVLADELDLAFTVDATD
                     ERLACHLLGYDPFVVLVAADDPIGAVATPADISDHPLIGQPSSNVCQRLIDQRLVTAG
                     IRADYVFRSQDNGAVQGMVRSGMGRAIMPLLAVDPDDPGVRVLPLDPPVASRTIQLTR
                     RLGRSLPPAADRFAAIAEEVCADRLRPEPAVAV"
     gene            5991..6260
                     /locus_tag="VLR27_02530"
     CDS             5991..6260
                     /locus_tag="VLR27_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02347.1"
                     /translation="MTRLPTTEALIVLADAVVTAGFDANRPALDEVVASARQRGIRPV
                     LADIVEDDSAPRPVRERALGRLLVALAAAPAPVDGPVSLVTASAA"
     gene            6331..7578
                     /locus_tag="VLR27_02535"
     CDS             6331..7578
                     /locus_tag="VLR27_02535"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HSP02348.1"
                     /translation="MGSSAWEAVLDRIDDAAAVADVSAGVHARLRHPERVLQVSIPVR
                     MDDGSLEVFTGWRVHHNTVRGPGKGGIRFHPDVDAEEVTALAAQMTLKTAVCGIPFGG
                     AKGGVRCDPRQMSAGELERLTRRYTTGIAPLLGPDRDVPAPDINTDGRVMAWLLDTLQ
                     MTGGGIHLPAAVTGKPLSVGGTRAHAGGTSAGVVVCARAVFEALGMELPGRRAVIQGF
                     GKVGGPLAFLLNSAGMRIVGVSDVTGAVINEGGLDVAELSDHVKATGGVAGFPLGDPI
                     DPDDLLALPCELLVPAALGGVIDAAVAERIQAKVIVEAANGPTLSAADAVLDRRGIAV
                     VPDVLANAGGVTASYFEWAQARQGYPWDIALTADRHHQVMAEAFDEVWAASQRWDVSL
                     RRAAYALAIDRVAAALEARGIFP"
     gene            complement(7636..9876)
                     /gene="katG"
                     /locus_tag="VLR27_02540"
     CDS             complement(7636..9876)
                     /gene="katG"
                     /locus_tag="VLR27_02540"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HSP02349.1"
                     /translation="MTEPQDMVDKGDTQSTSESENPAIDAPEPKTSGRPRSNQDWWPN
                     QLDLSVLHQHSERGNPMDPDFDYKAAVAELDVDALKQDIVDVMHTSQDWWPADYGHYG
                     PLFIRMSWHQAGTYRIEDGRGGGGDGSQRFAPLNSWPDNANLDKARRLLWPVKQKYGN
                     KISWADLLVLAGNVALEDMGFETFGFAFGREDIWEPEEIFWGPEDAWLGDERYADDGP
                     LDFGDQPFGAVTMGLIYVNPEGPKGNPDPVAAAHDIRLTFARMAMNDEETVALIAGGH
                     TFGKTHGAGDPDLIGAEPEGCPVHSMGLGWKNQHGTGKGADTITSGLEGAWTPTPTTW
                     DNSFWDMLFRFEYELRESPAGAKQWHPKEPEAQELVPDAHLEGVKHPPMMATTDLALI
                     ADPEYRKISERFRDNPDQFADAFARAWYKLLHRDMGPHSRFLGPWVAEPQLWEDPVPA
                     VDHELVDDAAVADLKAKVLDTGLSVQDLVFTAWASASSYRDTDKRGGANGARIRLAPQ
                     KDWEANDPQRVGPVIEALEGIQQDFNSSQSGRTRVSLADLIVLAGCAAVEKAAKDGGH
                     EVTVPFAPGRTDATDELTDAESFDVLEPKGDGFRNYLRAGDKLPPEQRLLDKANLLRL
                     CAPEMAVLVGGLRAIGANHGGTAHGVLTDRPGALPNDFFVNLLDPGTEWETATVGEGV
                     YEGKDRSTGETKWTATAADLVFGSNSQLRAIAEVYGQGDGEAKLVQDFVAAWDKVMNS
                     DRFDLR"
     gene            10022..>13395
                     /locus_tag="VLR27_02545"
     CDS             10022..>13395
                     /locus_tag="VLR27_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HSP02350.1"
                     /translation="MGFNNPGVSWSELERTLSDRHRKPTRAEGDGGDSPAWSYHRPSY
                     VPPADLQRRSATVPYAELHCHSNFSFLDGASHPETLAEEATRLGLEALALTDHDGFYG
                     VVRFAEAARVAGLPTVFGAELSLGLTQPQNGIADPEGSHLLVLARGPAGYARLADTIS
                     TAQLAGAEKGKPDYSGIDWSRAHGGEWLVLTGCRKGVVNQALHAHGPAAAEREVRRLV
                     DTFGAGNVVVELWDHHDPLDSVRNDALAAIADRVGVEVVATNNVHYATPGDRRLATAL
                     AAVRARRSLDDADGWLPPAATAHLRSGEEQARRFARYPGAVERAAELGRACAFDLHLV
                     APNLPPFPCPDGLDEMGYLRRLTEEGATHRYGPRHAEREPGAWAQIDHELALIETLGF
                     PGYFLIVWDLVDFCRRAGIYCQGRGSAANSAVCFALGITKADAVKLELLFERFLSPER
                     DGPPDIDIDIESNRREEVIQYVYERHGRRHAAQVANVITYRARSSIRDMAKALGYATG
                     QQDAFAKAADAWGNIASTMSQPGAAEELPADVVELAAEVEHFPRHLGIHSGGMVLCDR
                     PVVEVCPVEWGRFQGREGATVRADVPIRSVLQWDKDDCAAAGLVKFDLLGLGMLTALH
                     EAIDLVQAAYGIELDLAEIPQDDEVYAMLGTADSVGVFQVESRAQMATLPRLRPRTFY
                     DLVVEVALIRPGPIQGDSVHPYIRRRNGQEEVTYLHPLLEKSLAKTLGVPLFQEQLMQ
                     MSIDVGGFTPAESDQLRQAMGSKRSPERMERLRERFAAGARERDVPEDVIEQIWRKLA
                     AFARYGFPESHSVSFAYLVYASSWLKRFHPAAFCAGLLNAQPMGFWSPHSLIQDARRH
                     GVEVRTPDVSTSAAKATLVWDATPSRSGLHPRPPGGTSVGQNEPPETTTRGLPAEVWG
                     KGGPVVQLGLREVRHIGDDVARQIEAARDAGGPFASMEDLARRVPLTLPQLEALATAG
                     AFGCFRSPRTGEPLSRREALWQAGSVAQGGVDRLPGIVTGTVAPTLPGMDDFEEARAD
                     LWSTGMSPDGHPTRFIRDDLDRLGVVTSAGLWDVEPGAKVLIGGVVTHRQRPATAGGT
                     MFLNLEDETGLINVVVSRGCWNAHRKVALSAPAMLIR"
BASE COUNT         1694 a         5009 c         4668 g         2024 t
ORIGIN      
        1 gacctgatgc tgccggcctt cgacgagatc cgggccgact tcgacctcgg cgccggctcc
       61 accgcggtca ccggtctcgt cactgcctac ttcgtgggcc tcggcctcgg cacggtggtc
      121 tacgggcccc tcgccgacca caagggccgt cgctggacca tgcgcctcgg cctcggcatc
      181 tacatcgccg gcgccctgct ggccgcggtg gcgcccacgc tgccgatggt catgctcgct
      241 cgcttcctct ggggcatggg cgccgccggt ccccgcgtcg tggccctcgc catcgtgcgc
      301 gaccgcttcg agggcgacgc catgtcccgg acgatgtcgt cgctgatggc cgtgttcctc
      361 ctcgtccccg tgctcgcccc gaccctcggc gcgctgctgc tcgaggtcgc gccgtggcgc
      421 tggctgttcg tgctctgcgc cgtcggcgcc ctgctggtcg cgctgtggac gcagcggctc
      481 cccgagaccc tggccgccga gcaccgcatc cccgacctgc gcttccaccg ggtcaagacc
      541 gcgacccggc tggtgctgtc cagccgcacg gcggtcgcct acgccgccgc caccgtggcg
      601 ctctacggcg tgttcgcctc ctacctcggc tcctcggagg cgatcatcgg tcaggccctc
      661 gacagcgagg cgcagttccc gctgatcttc ggcgccctcg cgggctccat gggcgtggcc
      721 gccgtgctga acggtcggat cgtctcccgg ttcggcaccc ggtccatggt gcgcggcgcg
      781 ctgtcggtgt acgtggtgat gaccgtcgtg ctcctcgccg tcgccgtggt gaccgcggga
      841 cagccggcgc tctgggtgtt catgccggtg atggcgctcc tgctggcgag ccacgcccag
      901 ctgctcccga acctcaacac cctcgccatg gcgcccatgg cggcggtcgc cggcacggcg
      961 tcctcggtca tcggcgccgc gcagctcacc ctcggtgccg tgctcggcgg ggtgctcgac
     1021 cgcttcttcg acggcacgat cctgccgctg tccatcggct tcgcgctggc gagcgtgctc
     1081 accgtcgccg ccgtcacggt cgcccgccgc gtgccggccg aggacgtcga cctggtcgtc
     1141 gccggcgccg aaccctccac ctgacgccgg gcgcggggcg gcacctctcg gtactgtccg
     1201 tgcgaactcg agctgtccgg ggggacacag acatggactt ccacttcgcg tccgcatggg
     1261 agcgcgtggc cgacacggtg cccgacgtcg aggccgtcgt catgggcgac cagcgctgga
     1321 cctacgccgc cttcgacgac gccgcggcgc gcttcgcctc ggcgatggag gccgccggcg
     1381 tgtcccgtgg atcgcaggtg tcgctctacc tgcacaactg ccccgagtac ctgatcgccc
     1441 agcacgccgc gttcaagcac ggctgcggtc cggtgaacgt caactaccgg tacctcgacg
     1501 acgagctggt gtacctcgtc gacaacagcg acagcgaggt gctggtcttc cacgcgtcgc
     1561 tcggcgaccg ggtgggtcgg gtgcgcgacc gcctcgatcg ggtccggctg ttcgtcgtgg
     1621 tcgacgacga cggcaccgcc gccgagcacg acttcgcttc cgggttccac gacctcgtcg
     1681 ccgcccacga gccccaggcc cgctgggacc gctcgccgca ggacctgtac atgctctaca
     1741 ccggtggcac gaccggcctg cccaaggggg tcatgtacga caacggcgtc ttctgcaacg
     1801 ccctgctcgc cggcgccgcc ctgctgaacg gcctgccggc gccggcgtcg ctcgacgacg
     1861 tcggtgacct ggtccgcggc gtgcgggagc tcggtcccgt ggtgtcggtg ccgtgctgcc
     1921 cgctcatgca cggcaccggc atgtgggtcg gggccatgcc ggcgctcaac gtcggcggca
     1981 ccgtcgtgct gctgcagtcg cgcagcttcg acgccgacga gctcctcacc gtcatcgagg
     2041 ccgagggcgt gacccggtgc gccatcgtcg gcgacgcctt cgcccgtccc atcctccggg
     2101 cgatggaggc cgccgacgcg cgcggcgagc tgcccgacct ctcgtcgctc aagaccatca
     2161 gctcctccgg cgcgatgtgg agcgcggaga tcaaggacgg cctcacccgc ttcacctccg
     2221 ccgtgctgtc cgacggcctc ggctccaccg agggcggtgg ctacgggacg gtgaccgccg
     2281 accgcaaggc caagggcacg acggcgcggt tcaccccgac gccggacacc ctcgtgctcg
     2341 gtcccgacga cctgcccgtc acgccgggat cggaggaacc gggcctgctg gccacgcgca
     2401 ccggcgccca cgggtactac aaggatccgg agaagaccgc ccggacgttc aagcagatcg
     2461 acgggaacgg ctacgtgatc accggcgact gggccaccct ccacgaggac ggcaccatca
     2521 ccctccacgg tcgcggctcg aactgcatca actcgggagg cgagaagatc ttccccgagg
     2581 aggtcgagga ggcgctcaag acccacgccg gcgtcgacga ctgctacgtg gtcggcatcc
     2641 ccgacgcccg cttcggtcag cgggtggtgg cggtggtcgg gtgcgcggcc ggcgccgaac
     2701 cgcccgccga ggacgacctg cgcagccacc tccgcgaggt cctcgccagc tacaagatcc
     2761 cccgccagat cgtggtggtg gaccacctcc accgggcacc caacggcaag gccgactacg
     2821 gatgggcccg cgagcaggtg ctcgccaccc acgcgtaggc acccgacccg gggcgcgggg
     2881 cgcggtcccg gccgcctggc gccgtagcgt gcaccgccgt gcccacccgc cgtgcgaact
     2941 ggcgaacgct gcgccacccc gcgcagtacg tggcggtcgc gttcctgggg gccagcctgc
     3001 tcggtgggtt gctgctgtgg ctgcccttcg cgaccgaacc gggggagtcc acctccttcg
     3061 tcaccgcgct gttcaccgcc acgtccgcgg tgtgcgtgac gggcctgaac gtggtcgatc
     3121 cggcctcgca ctggtcgacg ttcggcgagc tggtcatcct cggcctgatc cagatcggcg
     3181 ggttcgggat catcgccctg tcgtcgctgc tggtcgcggt gctggcccgt cgcctcgggc
     3241 tgcggcaccg gctgatcgcc gtggccgaga ccggcgcgct cgacatgtcc gacgtgcgcc
     3301 gcctgctggt caacgtcgcc aagctgaccc tggtggtgga ggtggtcggc gccgtgctgc
     3361 tcgccggtcg cttcgctgcc acccacggcg agtccgtccc ccgcgccgcc tacctcgggg
     3421 tgttccactc gatctccgcg ttcaacaacg ccggcttcgc cctgttcgcc gacagcctcg
     3481 tcgggtacca gcgggacagc tacatcctcg tggtcatcgc cgtcgccatc atcatcggcg
     3541 gcctcggact gcccgtgtgg tcccagctcg gtcgccaccg gttccgcgcg cgctcgtggt
     3601 cgctgcacgc caagctcacg gtgaccacgg tcgtcgcgct gatcctcggt gggtgggggc
     3661 tcttcgcctg gttcgagtgg acgaaccccg acacgctcgg gcagctctcg gcgtgggact
     3721 ccacgatcaa cgccttcttc cacagcgtca ccccccgcac cgcgggcttc aacagcgtgg
     3781 acatcgcctc gctccgggag ccgtcccggc tgctcaccga ggtgctgatg ttcatcggcg
     3841 gcgggagcgc ctccaccgcc ggcggcatca aggtcacgac cttcgccgtc ctcgggtgga
     3901 tcatgtgggc cgaagcccgt ggtgaccccg acgtcgtcgt gttcgagcgc cgcgtccccc
     3961 aggccgcgca gcgacaggcc ctgacggtcg cgctcctcgc catcggcgtg gtgatcggct
     4021 ccaccatgtt gctgctggcc acctcgcccc tgggtcgcgc cgacctgatg ttcgaggtca
     4081 tctcggcgct cgccaccgtc gggctgtcgg cgaacatcac cccgctgctg tccgtcacct
     4141 cccaggtcgt gatcatcgcg ctgatgatcg tcggccgggt cggacccatc accctgttcg
     4201 ccgcgctcgt gctccgtgag cacgaccggc tctaccgcca tcccgaggag cgtccgatca
     4261 ttggctgacc acccccgacc acgccgcaac cgcccagcgc tgcagcgctc gaagagctcg
     4321 caggtcctcg tcgtcggcct cggccgcttc ggcaccgccg tggcgctcga gctgacccag
     4381 ctcgggttcg aggtcctcgg cgtcgacacc tcggcgcgcc gcgtccagaa gctggccgac
     4441 gacctcaccc acgtcctcga ggccgacacc accgaccccg aggtgctcac ccagatcggt
     4501 gcgcgcgact tcgagaacgc ggtcgtcggc atcggctccg acatcgaggc cagcatcctc
     4561 accaccgccg ccctcgccga cctcggcgtg cccaacatca tcgccaaggc ggtgaccacg
     4621 ccccacggac ggatcctgga gcgggtgggc gcggactccg tcgtctaccc cgagcacgag
     4681 atgggccggc gggtggcgca catggtgggg ggcagcatcc tcgactggtt ccagctcgat
     4741 gcgaacttcg ccatggtcga gacccacgcc ccggccgaga ccctcggtcg gaccctggcc
     4801 gagctcgagc tgcgcgcccg ctacggggtc acggtggtgt gcgtgaagcc cggcgaggag
     4861 gggttcacct acgccacggc cgacaccgtg ctggaggagg gcgacgtgct ggtggtggcc
     4921 gggccgggca aggagtgcga ggccttcgcc cgcatgtcct gaggtcagac cgctgtcctg
     4981 aggttcagac tgcgacggcc ggctcgggtc ggagccggtc ggcgcagacc tcctcggcga
     5041 tggctgcgaa gcggtcggcc gccggcggca ggctccggcc cagccgccgg gtgagctgga
     5101 tggtgcggga tgccacgggc gggtcgagcg ggaggacccg gaccccgggg tcgtccgggt
     5161 cgacggccag caacggcatg atcgcccggc ccatgcccga gcggaccatg ccctgcaccg
     5221 caccgttgtc ctgggagcgg aagacgtagt cggcccggat gcccgcggtg acgaggcgct
     5281 ggtcgatgag ccgctggcac acgttggacg acggctgccc gatgagcggg tggtcgctga
     5341 tgtcggcggg ggtggcgacc gcgccgatgg ggtcgtcggc ggcgacgagg accacgaagg
     5401 ggtcgtagcc gagcaggtgg caggccaagc gctcgtcggt ggcgtcgacg gtgaaggcga
     5461 ggtcgagctc gtcggccagg acccgttcga ccagggcggt ctggtcgtcg gtctcgaaga
     5521 gcccgatgtc gacctggggc acctcggccc gcatccgtcc gacgatgggt gggaggatct
     5581 cggccgacac cgactggaac gtgccgatga ccaggcggcc ggtgacgccc cgccgcagct
     5641 ggtcgagctc cgcgctcacc gcctcgatcc ggtcgagcac gtcgcgggcg tggtccagca
     5701 gcagccggcc agcgggtgtg agctcggcgg gccggggacc cttgggccgg tcgagcacgg
     5761 gcaggcccac cgctcgctcc agcgacgcga tctgctggct caccgcggac tgggtgaacc
     5821 cgagcgcggt ggccgctcgg gcgaaggagc cctcctcggc gacggcgacg agggcggcga
     5881 ggtggcggat ctcgaggtcg gggaacgcag gcacgaacat cagcatacct gatgtacagg
     5941 agcagagatc atcgctggtg ctgatgaagg gatcgtcgta cgttcgatcc atgacccgcc
     6001 tgcccaccac cgaagcgctc atcgtcctcg ccgacgccgt cgtcaccgcc ggcttcgacg
     6061 ccaaccgtcc cgccctcgac gaggtcgtcg cctctgcccg gcagcggggc atccgccccg
     6121 tgctcgccga catcgtcgag gacgactccg ccccgcgccc cgtgcgcgag cgcgccctcg
     6181 ggcgactgct cgtggcgctg gccgcagcac cggccccggt cgacgggccg gtctcgctgg
     6241 tgacggcctc ggccgcctga ccccggagcc ggtcctgccg gtcctgccgg gtcgccgcgc
     6301 gccgcgtgcg gcgaatgcca cactcgggcc gtggggtcct cagcttggga agccgtcctc
     6361 gatcgcatcg acgatgccgc ggcagtggcc gacgtcagcg ccggcgtgca cgctcggttg
     6421 cgtcaccccg agcgggtgct ccaggtctcg atccccgtgc gcatggacga cggctcgctc
     6481 gaggtgttca ccggttggcg cgtgcaccac aacacggtgc gcggcccggg caagggcggc
     6541 atccgcttcc atcccgacgt cgacgccgag gaggtcaccg ccctcgccgc ccagatgacg
     6601 ctgaagaccg cggtgtgcgg catccccttc ggcggggcca agggcggggt ccgctgcgat
     6661 ccgcgccaga tgagcgcggg cgagctcgag cggctgacgc gccgctacac gaccggcatc
     6721 gccccgctgc tcgggccgga ccgggacgtg cccgcgcccg acatcaacac cgacgggcgg
     6781 gtcatggcgt ggctgctcga caccctgcag atgaccggcg gcgggatcca cctgccggcc
     6841 gcggtcaccg gcaagccgct gtccgtgggc ggcacgcgcg cccatgccgg cggcacgtcc
     6901 gccggcgtgg tggtctgcgc ccgggccgtc ttcgaggcgc tcggcatgga gctgcccggg
     6961 cggcgcgccg tcatccaggg cttcggcaag gtgggcgggc cgctcgcctt cctgctgaac
     7021 tcggccggca tgcggatcgt gggcgtcagc gacgtcaccg gtgcggtgat caacgagggc
     7081 ggcctcgacg tggccgagct gtccgaccac gtgaaggcca ccggcggcgt cgccggcttc
     7141 cccctcggcg atcccatcga ccccgacgac ctgctggcgc tgccgtgcga gctcctggtg
     7201 ccggccgcgc tcggcggggt catcgacgct gccgtcgccg agcggatcca ggccaaggtg
     7261 atcgtcgagg cggccaacgg cccgacgctg agcgcggccg atgccgtgct cgatcgccgg
     7321 gggatcgcgg tggtcccgga cgtgctcgcc aacgccggtg gcgtcaccgc gtcctacttc
     7381 gagtgggctc aggctcgcca gggctacccg tgggacatcg ccctcaccgc tgaccgccac
     7441 caccaggtca tggccgaggc gttcgacgag gtgtgggctg cgtcccagcg ctgggacgtc
     7501 agcctgcgcc gggccgccta cgccctggcg atcgatcgcg tcgccgcggc gctggaggcc
     7561 cggggcatct tcccctgagc ctcgccggcg gccggccccg atgggggacc gaccgccgga
     7621 tcggcctcga tccgctcagc gcaggtcgaa gcggtccgag ttcatgacct tgtcccaggc
     7681 ggccacgaag tcctgcacca gcttcgcctc gccatcaccc tgcccgtaga cctcggcgat
     7741 ggcccgcagc tgggagttcg agccgaagac caggtcggcc gcggtggccg tccacttcgt
     7801 ctcgccggtg ctgcggtcct tgccctcgta gaccccttcg ccgaccgtgg cggtctccca
     7861 ctcggtgccc ggatcgagga ggttgacgaa gaagtcgttg ggcagcgcgc ccggccggtc
     7921 ggtgagcacg ccgtgcgcgg tcccgccgtg gttggcgccg atggcccgga gcccgcccac
     7981 gaggacggcc atctccggtg cgcagaggcg caggaggttc gccttgtcga gcaggcgctg
     8041 ctccggcggc agcttgtcgc cagcccggag gtagttccgg aacccgtcgc ccttgggctc
     8101 gagcacgtcg aaggactcgg cgtcggtcaa ctcgtcggtg gcgtcggtgc ggcccggcgc
     8161 gaacggcacc gtgacctcgt gcccgccgtc cttggccgcc ttctcgaccg ctgcgcagcc
     8221 ggcgaggacg atgaggtcgg cgagcgagac ccgcgtgcgg ccggactgcg agctgttgaa
     8281 gtcctgctgg atgccttcga gggcctcgat gaccggtccc acccgctggg gatcgttggc
     8341 ctcccagtcc ttctgggggg cgaggcggat gcgagcaccg ttggcgccgc cccgcttgtc
     8401 ggtgtcccgg taggacgagg cagatgccca tgcggtgaac accaggtcct ggaccgacag
     8461 cccggtgtcg aggaccttgg ccttcaggtc ggccacggcg gcgtcatcca ccagctcgtg
     8521 gtcgacggcg gggaccgggt cctcccacag ctggggctcc gccacccacg gaccgaggaa
     8581 ccgcgagtgg gggcccatgt cgcggtgcag cagcttgtac caggcccggg cgaacgcatc
     8641 ggcgaactgg tcggggttgt cccggaaccg ctcggagatc ttgcggtact ccgggtcggc
     8701 gatgagcgcg aggtcggtgg tggccatcat cggcgggtgc ttcacgcctt cgaggtgggc
     8761 gtcgggcacc agctcctggg cctccggctc cttggggtgc cactgcttgg cgccggccgg
     8821 gctctcgcgg agctcgtact cgaagcggaa cagcatgtcc cagaagctgt tgtcccacgt
     8881 cgtgggggtg ggcgtccagg cgccctccag gccgctggtg atggtgtcgg cgcccttgcc
     8941 ggtgccgtgc tggttcttcc agccgaggcc catcgagtgg acggggcagc cctcgggctc
     9001 cgcgccgatg aggtcggggt cgccggcacc gtgggtcttg ccgaacgtgt ggccaccggc
     9061 gatgagcgcg accgtctcct cgtcgttcat ggccatccgg gcgaaggtca accggatgtc
     9121 gtgggccgcg gcgaccggat cggggttgcc cttggggccc tcggggttga cgtagatgag
     9181 gcccatggtg acggcgccga agggctggtc accgaagtcg agcgggccgt cgtcggcgta
     9241 gcgctcgtcg cccagccagg cgtcctcggg gccccagaag atctcctcgg gctcccagat
     9301 gtcctcccgg ccgaacgcga agccgaaggt ctcgaagccc atgtcctcga gggcgacgtt
     9361 gccggcgagc acgagcaggt cggcccacga gatcttgttg ccgtacttct gcttgaccgg
     9421 ccacagcagg cggcgggcct tgtcgaggtt ggcgttgtcc ggccagctgt tcagcggggc
     9481 gaatcgctgc gagccgtcgc cgccaccgcc gcgcccgtcc tcgatgcggt acgtcccagc
     9541 ctggtgccag ctcatgcgga tgaagagcgg gccgtagtgg ccgtagtccg cgggccacca
     9601 gtcctgcgac gtgtgcatga cgtcgacgat gtcctgcttg agcgcatcga cgtcgagctc
     9661 ggcgaccgcg gccttgtagt cgaagtccgg atccatgggg ttgccgcgct cgctgtgctg
     9721 gtgcaggacg ctcagatcga gctggttcgg ccaccagtcc tggttgctcc ggggccggcc
     9781 gctggtcttg ggctccggcg catcgatggc cgggttctcg ctctcgctgg tgctttgggt
     9841 gtcgcccttg tcgaccatgt cctggggttc tgtcatgtcc ctgtcctctt cctcgtcgcg
     9901 gcggtggtgc tcctgaccct gtgctatcag ccacggggcg gcctgtctcc gcgatcgctc
     9961 ctatgggggt cataaccagg gcctctggtg gtcgaacacc tgttcgtcta ccgttcctga
    10021 cgtgggcttc aacaacccgg gcgtctcctg gtccgagctg gagcggacgc tctccgaccg
    10081 gcaccgcaag cccacccgcg ccgagggcga cggtggcgac agcccggcct ggtcctacca
    10141 ccgcccgtcc tacgtgccgc cggccgacct gcagcgacgc tccgccaccg tcccctacgc
    10201 cgagctgcac tgccactcca acttcagctt cctcgacggc gcctcccacc ccgagaccct
    10261 ggccgaggag gccacccgcc tcggcctcga ggccctggcc ctcaccgacc acgacggctt
    10321 ctacggtgtg gtccgcttcg ccgaggcggc ccgggtggcc ggcctgccca ccgtgttcgg
    10381 ggccgagctg tccctcgggc tgacccagcc ccagaacggc atcgccgacc ccgagggctc
    10441 ccacctgctg gtgctggccc ggggcccggc cggctacgcc cggttggccg acaccatctc
    10501 caccgcccag ctcgccggcg ccgagaaggg caagcccgac tactccggca tcgactggtc
    10561 ccgggcccac ggcggcgagt ggctcgtcct caccggctgc cggaaggggg tggtcaacca
    10621 ggcgctgcac gcccacgggc cggcggcggc cgagcgggag gtgcgccgcc tcgtcgacac
    10681 cttcggcgcc ggcaacgtgg tggtcgagct gtgggaccac cacgacccgc tggactcggt
    10741 gcgcaacgac gcgctcgccg ccatcgccga ccgggtcggg gtcgaggtcg tggccaccaa
    10801 caacgtccac tacgccaccc cgggcgatcg tcgcctggcc acggcgctgg ccgcggtgcg
    10861 ggcccgccgg agcctcgacg atgccgacgg ctggctgccc cctgcggcca ccgcccacct
    10921 ccgctccggc gaggagcagg cccggcgctt cgcccgctac cccggcgcgg tggagcgggc
    10981 cgccgagctg ggccgggcct gcgcgttcga cctgcacctg gtggccccga acctgccgcc
    11041 gttcccgtgc cccgacggcc tcgacgagat ggggtacctg cgccgcctca ccgaggaggg
    11101 cgccacccac cggtacgggc cgcggcacgc cgagcgcgag ccgggggcgt gggcccagat
    11161 cgaccacgag ctcgccctca tcgagaccct cggcttcccc ggctacttcc tcatcgtgtg
    11221 ggacctggtc gacttctgcc gcagggccgg gatctactgc cagggccggg ggagcgcggc
    11281 gaactccgcg gtgtgcttcg ccctcggcat caccaaggcc gacgccgtga agctggagct
    11341 gctgttcgag cggttcctgt cccccgagcg cgacggcccg ccggacatcg acatcgacat
    11401 cgagtccaac cggcgcgagg aggtcatcca gtacgtctac gagcgccatg gccgccgcca
    11461 cgccgcccag gtcgccaacg tcatcaccta ccgggcccgg tcctcgatcc gggacatggc
    11521 caaggcgctc ggctacgcca cgggccagca ggacgccttc gccaaggccg ccgatgcctg
    11581 gggcaacatc gcctccacca tgtcccagcc cggcgcggcc gaggagctgc cggccgacgt
    11641 ggtggagctg gccgccgagg tcgagcactt cccccgccac ctcggcatcc actccggcgg
    11701 catggtgctg tgcgaccggc cggtggtcga ggtctgcccg gtcgagtggg ggcgattcca
    11761 gggccgggag ggcgccacgg tgcgcgccga cgtgcccatc cgctcggtgc tgcagtggga
    11821 caaggacgac tgcgccgccg ccggcctcgt gaagttcgac ctgctcggcc tcggcatgct
    11881 caccgccctg cacgaggcca tcgacctggt gcaggccgcc tacggcatcg agctggacct
    11941 ggccgagatc ccccaggacg acgaggtcta cgccatgctc ggcacggccg actcggtggg
    12001 cgtgttccag gtcgagtccc gggcccagat ggccaccctg ccccggcttc gtccccgcac
    12061 cttctacgac ctggtggtcg aggtcgccct catccgcccc ggcccgatcc agggcgactc
    12121 ggtgcacccc tacatccgcc ggcgcaacgg ccaggaggag gtcacctacc tgcacccgct
    12181 gctcgagaag tcgctggcga agaccctcgg ggtgccgctg ttccaggagc agctcatgca
    12241 gatgtccatc gacgtcggcg ggttcacccc ggccgagtcc gaccagctcc gccaggccat
    12301 ggggtccaag cgctcgcccg agcggatgga gcgcctgcgc gagcgcttcg ccgccggcgc
    12361 ccgcgagcgc gacgtgcccg aggacgtcat cgagcagatc tggcggaagc tggccgcctt
    12421 cgcccgctac ggcttccccg agagccactc ggtgtccttc gcctacctgg tctacgcctc
    12481 gtcgtggctc aagcgcttcc acccggccgc gttctgcgcc ggcctgctca acgctcagcc
    12541 catggggttc tggtcgcccc actcgctgat ccaggacgcc cgccgccacg gcgtcgaggt
    12601 ccgcaccccc gacgtgtcga cctccgccgc caaggccacg ctggtgtggg acgccacccc
    12661 ctcccgttct ggcctacatc cacgaccacc aggtggcaca tcggtaggcc agaacgagcc
    12721 gccggagacc acgacgcggg ggctgccggc cgaggtgtgg ggcaagggcg gcccggtggt
    12781 gcagctcggc ctgcgcgagg tccgccacat cggcgacgac gtggcccgcc agatcgaggc
    12841 cgcccgcgac gccggtggac cgttcgcctc gatggaggac ctggcccgcc gggtgccgct
    12901 gaccctgccc cagctcgagg cgctggccac cgccggcgcg ttcggctgct tccgctcgcc
    12961 ccgcaccggc gagccgctct cccggcgcga ggcgctgtgg caggccggct cggtcgccca
    13021 gggcggggtc gaccggctcc cgggcatcgt caccggcacg gtggcgccga cgctgcccgg
    13081 catggacgac ttcgaggagg cccgggccga cctgtggtcc accggcatga gccccgacgg
    13141 ccacccgacc cgcttcatcc gggacgacct cgaccgcctc ggggtcgtga cctccgccgg
    13201 cctgtgggac gtggagccgg gcgccaaggt gctcatcggt ggggtggtca cccaccgcca
    13261 gcgacccgcc accgccggcg gcaccatgtt cctcaacctc gaggacgaga ccggcctcat
    13321 caacgtggtg gtgtcccggg gctgctggaa cgcccaccgc aaggtcgccc tcagcgcgcc
    13381 cgccatgctc atccg
//
LOCUS       DATISU010000057        10602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_95883, whole genome shotgun sequence.
ACCESSION   DATISU010000057 DATISU010000000
VERSION     DATISU010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 10602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10602
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95883"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VLR27_02550"
     CDS             complement(<1..592)
                     /locus_tag="VLR27_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02351.1"
                     /translation="MSNLPDQPFGTPRRWGVFVAGVASLLTGISLAITAELGVGSWQV
                     FETGLVEATGLSFGVVVLGEALAALALAWFWLGERPWIATLVLAFGGVVIGALLAVLS
                     TPDLLVARIALLGVGASLIAVGVAFYLAADLGPSAQDALFVGIYRRYEIRPAVVRFAL
                     DGSLVVGGFLLGGQLGAGTVAMTVLVPALIEPALRVG"
     gene            complement(576..1778)
                     /locus_tag="VLR27_02555"
     CDS             complement(576..1778)
                     /locus_tag="VLR27_02555"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSP02352.1"
                     /translation="MTTGTGPADAVDTVVGFARTLRAAGVHATPGRVQALIDSLAVLD
                     PTDRAHLYWAGRTSLCASHDDVERYDRAFDAYFGGEAPHERLRAIAPPRTSSLRPSLA
                     DALPPDGAEDDASVPVAVPDASATEQLRHRDFAAMGEAERAMVDLLLDRLRLPGELRR
                     TRRHRPGGARDLDRRRILRSVLRQGGEPGRLHRRERRHRPRRVVLVVDVSGSMSAYAT
                     ALLRFAHAASARGVTPTEVFTLGTRLTRVTTELAHRDPDTAMAAVSRAVPDWSGGTRL
                     GPLLKELLDRWGQRGLVRGAVVVVLSDGWERGDVALLDQQMGRMARLAHRVVWANPRK
                     AREGFEPTAAGLAAALPHVDDFVSGHSIAALEELADVVLGRRRAPVGTNRGSDGVDPY
                     RAPGVEPP"
     gene            complement(1775..2689)
                     /locus_tag="VLR27_02560"
     CDS             complement(1775..2689)
                     /locus_tag="VLR27_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSP02353.1"
                     /translation="MTPGPPGLLPAGVGTPADLRDRLADLRYLADDGLAIAAFLALRL
                     ARPLFLEGDPGVGKTEVAKTLATLLDAPLLRLQCYEGIDAAEALYDWDFPRQLLHLRA
                     TEHADVTADEVMREVYGEDFLLARPLLAALRTSPSVLLIDEVDRADDEFEAFLLEVLS
                     DWSVTIPELGTITAAHPPVVVITSNRTREVHDALKRRCYYHWLDHPDLARETAIVRAR
                     VPEAADRLVEQVARLTQALRSADLHKPPGVAETIDWTEALVALGATTLDAELATQTLG
                     AVLKYREDQERARSLDIGVMVQRAVAGA"
     gene            complement(2686..3537)
                     /locus_tag="VLR27_02565"
     CDS             complement(2686..3537)
                     /locus_tag="VLR27_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HSP02354.1"
                     /translation="MIPASFDYLAPTSVDEVVAALAEHGEDAKVLAGGHSLLPLMKLR
                     FAVPSVVVDIGKVEELRGVRDAGDHLAIGAGTTHHEIVHDPLVRQHCGLLAEATSMVG
                     DAQVRHRGTIGGATAHGDSAGDLPAVVSALQATFVAQGPGGRREIAAADFFQGYLETA
                     LAPDEVLVEVRIPKHDGWGFAYEKFNRVAQAWAVVGVAAMVRRENGSVAEARIGLTNM
                     GAVPVRATAAEEALAGADRTDDAIRAACQHAADGTSPPADLSAQPDYRQHLARVLSAR
                     AVARAAG"
     gene            complement(3534..5915)
                     /locus_tag="VLR27_02570"
     CDS             complement(3534..5915)
                     /locus_tag="VLR27_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSP02355.1"
                     /translation="MTVIDDRPADVGGVGTAPWRKEDAHLVTGQTTWTDNLAPVGTLH
                     MVFVRSTMAHARIGSIDTSEAEGMPNVVGVFTGADFADECPTLPCAWPVTPDMVHPAH
                     PPIAVGKVRFAGDPVAVVVARDRASAVDAAETVLVDLEPLPAVVDMEQALTDEVVLHE
                     DEGTNKAFFFSMPTGADVDETFAKADVVVKRRFIQQRLLPTPIEPRAVLCIPQAASGD
                     FTIYSATQIPHILRTMLAVVTGTPEHKIRVIAPDVGGGFGAKLQVTGEEVIALLVAKR
                     LGRPVKWTETRSETNLTMHHGRGQIQDIEIAATKDGKLLGLKVDLLADMGAYLMLVTP
                     GVPILGAFMYPAIYKMDAYRFNCTGVFTTKTPTDAYRGAGRPEATFAIERIMDELAAE
                     LDMDPMELRKLNWITHEEFPYEMVAGLTYDSGNYEAATARAMELFDYDGLRAEQAQRR
                     ADGDRIQLGIGISTFTEMCGLAPSRVLGQLDYGAGGWEAATVRMLPSGKVQVVTGSSP
                     HGQGHETTLSQITSSALGVPFDDIEVLHGDTQISPMGLDTYGSRSLVVGGIALQKAAG
                     KVVDKARKLAAHLLEASEDDIEFADGTFSVKGSPDASKSIQELALAIFAAHDYPEDME
                     PDLTGHAIFDPENFSYPHGTHLCAVEIDTETGHTSIRTYVAVDDVGKVINPQIVEGQV
                     HGGIAQGIAQALYEGVVYDDDGNLVTGTLVDYLVPGAPDLPTFTTDRTETPAANDLGV
                     KGVGEAGTIASTPAVVNAVVDALRPYGVHDVEMPCTPERVWRAIQSGQKGGAQ"
     gene            complement(5912..6421)
                     /locus_tag="VLR27_02575"
     CDS             complement(5912..6421)
                     /locus_tag="VLR27_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSP02356.1"
                     /translation="MARITVTVDGTAYSDDVEPRTLLVHHLREALGLTGTNVGCDTSN
                     CGACTVHVDGQSVKSCTMLAIQADGREVLTIQGLADTWDGEGSLHPLQQAFHEAHALQ
                     CGYCTPGMIMASLDLLEENPDPTDDEVRHGLEGNLCRCTGYQNIVKAVRAAAVQIRQG
                     AGASQEVPA"
     gene            complement(6621..6983)
                     /locus_tag="VLR27_02580"
     CDS             complement(6621..6983)
                     /locus_tag="VLR27_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02357.1"
                     /translation="MHVLTTAKTLGLEADDRGGLSGTIEESLVLAALEELASVHSDHA
                     DLFILASDRVRSSRSAGLEVLEANSVFGRYRDSNEIQLRYRGEMKEVFDDLLIDLALR
                     GVDMNDSEDDTTEVSSPS"
     gene            complement(7170..7940)
                     /locus_tag="VLR27_02585"
     CDS             complement(7170..7940)
                     /locus_tag="VLR27_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSP02358.1"
                     /translation="MSQPLLDGKRILVTGIANAGSIAHATAARAHELGATVVTTAMPR
                     DLVGAQDAAADIAADVAVLAMDATSPTDLEAVATWVGDELGGLDGALHAIAFAPKAAL
                     GSVAGVPAEAVELAFRTSVHTYVELGQLLADLAPPAGASLVGLDFDASRAWPTYNWMG
                     VCKSALEGANRYLARELGPRRVRSNLVAAGPLETRAASAIPGFDLLLDSWEQRAPLGW
                     DPHDAGPVADAVCFLLSDLARAVTGEILQVDGGHHAMA"
     gene            8076..8378
                     /locus_tag="VLR27_02590"
     CDS             8076..8378
                     /locus_tag="VLR27_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HSP02359.1"
                     /translation="MVSETPGWTFLTNHGHVLVCIANNAEVRISEIADQVGIGERAAQ
                     GIVTDLVEGGYVTRTRVGRRNHYAVHTSLPLRHPLERDHVVGELLRAVASPAAADD"
     gene            complement(8388..9407)
                     /locus_tag="VLR27_02595"
     CDS             complement(8388..9407)
                     /locus_tag="VLR27_02595"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSP02360.1"
                     /translation="MRERARQMATRAARSALGFAAERVAASDRLMDLVYDLMNETNFG
                     GLAEHEEMLSDSVRVEAYHRAIQRNVAPGDVVLDLGSGTGLLAFLASRAGAGKVYAVE
                     HGDVIEVAREIAGHNGFTNIEFVQANSREFTPPEPIDLVLHEQMGDELFNENMVQNVL
                     DLRDRVLRPGGRILPARFRLFVEPISMHEAMRVRRFWNIELPEGIDLAAMERSPIAGR
                     FDTGRNEQLWVRPGSVAATIGTPQPVLELDLHTLDGLDQLPTHHVVERTAGADAIVDG
                     ACVWFEAIFDDEVTLTTSPLATNTSWGNRMFRLDEEVGAGETLRLSVDLGRLVDASTW
                     SVTAI"
     gene            9629..9841
                     /locus_tag="VLR27_02600"
     CDS             9629..9841
                     /locus_tag="VLR27_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02361.1"
                     /translation="MAHRTRAAAEQLVPGNPVEVRTRFDGTWVPGYRVCGERSGRYVI
                     ERCSDGFELPVDFDLAELRPTAPNPG"
     gene            complement(9861..>10602)
                     /locus_tag="VLR27_02605"
     CDS             complement(9861..>10602)
                     /locus_tag="VLR27_02605"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HSP02362.1"
                     /translation="PRTLALALLAAVTFLAGCGDDADSEDRADQESTDTTAEAAEDGE
                     TGASGDAGEAGSLGDDVAAVVDGEEISAASIEGHVEDFAANPQLAEQFEGEDGDAARA
                     QLGAQILGSAIQSSVLVAGAEELDVPVTEEDIEAARAQVEEQAGGPEGLQAAMEQQGL
                     SEDLLQLELRGVAAVQNITDALDEAAGDDDPADTTPDEVEEGAELTPSEQRVQEHLLE
                     LATTAEIQVHPDFGRWDPQTGQVLPPGS"
BASE COUNT         1423 a         3697 c         3986 g         1496 t
ORIGIN      
        1 ggccgacccg cagggcgggc tcgatgagcg ccgggaccag gacggtcatg gccacggtcc
       61 cggccccgag ctggccaccc aggaggaacc ccccgacgac caggctgccg tcgagggcga
      121 agcggacgac ggcgggccgg atctcgtagc ggcggtagat ccccacgaac agggcatcct
      181 gcgcggacgg tccgaggtcg gcggcgaggt agaaggcgac cccgacggcg atcagcgatg
      241 cgccgacacc gagcagcgcg atgcgggcga cgaggaggtc cggcgtggac aggacggcga
      301 gcagggcgcc gatgaccacg ccaccgaacg cgagcacgag ggtcgcgatc cacggccgct
      361 cgcccagcca gaaccacgcc agggccagcg cggccagcgc ctcgcccagc acgaccacgc
      421 cgaagctgag cccggtggcc tcgaccagcc cggtctcgaa gacctgccac gacccgaccc
      481 cgagctcggc ggtgatggcg agcgagatgc ccgtgagcag ggaggcgacg cccgccacga
      541 acacgcccca gcggcgcggg gtgccgaagg gctgatcagg gaggttcgac accgggggca
      601 cggtaggggt caacaccgtc ggacccgcgg ttcgttccca ccggtgcccg gcgacgtccg
      661 aggacgacgt cggccagctc ctcgagggcc gcgatgctgt ggcccgagac gaagtcgtcc
      721 acgtggggga gtgcggcggc cagcccggcg gcggtgggct cgaagccctc ccgggccttg
      781 cgcgggttgg cccacaccac ccggtgggcg agccgcgcca tgcgccccat ctgctggtcg
      841 aggagggcga cgtcgccgcg ctcccacccg tcggagagga cgaccacgac ggcaccccgc
      901 acgaggccgc gctggcccca ccggtccagc agctccttga ggagcggccc gaggcgggtg
      961 ccgccgctcc agtccggcac cgcccggctg acggccgcca tcgcggtgtc gggatcgcgg
     1021 tgggccagct cggtcgtgac ccgggtgagg cgggtgccga gggtgaagac ctccgtcggc
     1081 gtgacgccgc gtgccgacgc cgcgtgcgcg aagcgcagca gggcggtggc gtaagccgac
     1141 atggagccgc tcacgtcgac cacgagcacc acccggcggg gccggtgccg ccgctcccgg
     1201 cggtggagcc ggcccggctc gccgccctgg cgcaggacgc tgcgcaggat ccggcgacgg
     1261 tcgaggtcgc gcgcaccacc cgggcggtgc cggcgggtgc gccgcagctc accggggagg
     1321 cgcaggcggt ccagcagcag gtcgaccatc gcccgctcgg cctcgcccat ggcggcgaag
     1381 tcccggtgcc ggagctgctc cgtggcggac gcgtcgggga ccgcgaccgg gacgctggcg
     1441 tcgtcctcgg cgccgtcggg tgggagggca tcggcgaggg acgggcgcag cgagctggtc
     1501 cggggcggtg cgatcgcccg gagccgctcg tggggggcct cgccgccgaa gtaggcgtcg
     1561 aaggcgcggt cgtagcgctc gacatcgtcg tggctcgcgc acaggctggt gcgcccggcc
     1621 cagtagaggt gggcgcggtc ggtggggtcc agcaccgcca gggagtcgat gagcgcctgc
     1681 acccggccgg gagtggcgtg cacgccggcg gcgcgcaggg tccgggcgaa gccgacgacc
     1741 gtgtcgaccg cgtcggcggg tccggtcccg gtggtcacgc cccggcgacg gcccgctgga
     1801 ccatcacccc gatgtccagc gaccgggccc gctcctggtc ctcccggtac ttcagcaccg
     1861 cgccgagcgt ctgggtggcc agctcggcgt cgagcgtggt cgcgccgagc gcgaccagcg
     1921 cctcggtcca gtcgatggtc tcggccacgc cgggtggctt gtggaggtcg gcgctccgca
     1981 gcgcctgggt gagccgggcg acctgctcca cgagccggtc cgccgcctcc ggcacccggg
     2041 cccggacgat cgcggtctcg cgggccaggt ccgggtggtc gagccagtgg tagtagcagc
     2101 gccgcttgag cgcgtcgtgc acctcgcggg tgcggttcga ggtgatgacc accaccggcg
     2161 gatgcgcggc ggtgatggtg ccgagctcgg ggatggtgac cgaccagtcc gacagcacct
     2221 cgagcaggaa ggcctcgaac tcgtcgtcgg cccggtcgac ctcgtcgatc agcagcaccg
     2281 aggggctggt gcgaagggcg gcgaggagcg gccgggcgag gaggaaatcc tcgccgtaca
     2341 cctcgcgcat cacctcgtcg gcggtcacgt cggcgtgctc ggtggcgcgg aggtgcagca
     2401 gctggcgggg gaagtcccag tcgtagagcg cctcggccgc gtcgatgccc tcgtagcact
     2461 ggaggcgcag cagcggcgcg tcgagcaggg tggcgagggt cttggccacc tcggtcttgc
     2521 cgacgccggg gtcgccctcg aggaacagcg ggcgggccag gcgcaaggcc aggaacgcgg
     2581 cgatcgccag gccgtcgtcg gcgaggtagc gcaggtccgc cagccgatcc cgcagatcgg
     2641 ccggcgtccc cacgcccgcg ggcagcagcc cgggcggccc cggggtcaac ccgcggctcg
     2701 ggccacggcc cgggccgaca gcacccgcgc caggtgctgg cggtagtcgg gctgggccga
     2761 gaggtcggcc ggcgggctgg tcccgtcggc ggcgtgctgg cacgcagccc ggatcgcgtc
     2821 gtcggtgcgg tcggcaccgg cgagcgcctc ctccgccgcc gtcgcccgca ccggcaccgc
     2881 ccccatgttg gtcagcccga tgcgggcctc ggcgaccgag ccgttctccc ggcggaccat
     2941 cgccgccacg ccgacgaccg cccacgcctg ggcgacccgg ttgaacttct cgtaggcgaa
     3001 gccccagccg tcgtgcttgg ggatccgtac ctcgaccagg acttcgtcgg gggccagcgc
     3061 ggtctccagg tagccctgga agaagtccgc cgccgcgatc tcccttcggc cgcccggccc
     3121 ctgggccacg aacgtcgcct ggagcgcgga cacgaccgcc ggcaggtcac cggccgagtc
     3181 gccgtgggcc gtggcgccgc cgatggtgcc gcggtggcgg acctgggcgt cgccgaccat
     3241 ggacgtggcc tcggccagca ggccgcagtg ctggcgcacc agcggatcgt gcacgatctc
     3301 gtggtgcgtg gtgccggcac cgatggccag gtggtcgccg gcgtcgcgga cgccacgcag
     3361 ctcctcgacc ttgccgatgt cgacgaccac cgacggcacg gcgaagcgca gcttcatcag
     3421 cggcagcagg ctgtgcccgc cggccagcac cttggcgtcc tcgccgtgct cggcgagcgc
     3481 ggccacgacc tcgtcgaccg aggtcggggc caggtagtcg aacgatgcgg ggatcactgc
     3541 gcacccccct tctggccgga ctggatcgct cgccacaccc gttcgggcgt gcacggcatc
     3601 tccacgtcgt gcacgccgta cgggcgcagc gcgtcgacca cggcgttgac caccgccggc
     3661 gtcgatgcga tggtgccggc ttcgccgacc cccttgaccc cgaggtcgtt ggccgccggg
     3721 gtctcggtgc ggtccgtcgt gaacgtcggc aggtcgggcg caccggggac caggtagtcc
     3781 accagcgtgc cggtgaccag gttgccgtcg tcgtcgtaga ccacgccctc gtagagcgcc
     3841 tgggcgatcc cctgggcgat gccaccgtgg acctgcccct ccacgatctg ggggttgatg
     3901 accttgccca cgtcgtccac cgcgacgtag gtgcggatgg acgtgtggcc cgtctcggtg
     3961 tcgatctcca cggcgcacag gtgggtgccg tggggatagg agaagttctc ggggtcgaag
     4021 atggcgtgcc cggtgaggtc cggctccatg tcctcggggt agtcgtgcgc cgcgaagatg
     4081 gccagcgcca gctcctggat ggacttggac gcatcgggcg agcccttcac cgagaaggtg
     4141 ccgtcggcga actcgatgtc gtcctcggag gcctcgagca ggtgggcggc gagcttgcgg
     4201 gccttgtcga cgaccttccc agccgccttc tggagggcga tgccgcccac caccagcgac
     4261 cgggagccgt aggtgtccag gcccatcggg gagatttggg tgtcgccgtg gagcacctcg
     4321 atgtcgtcga agggcacgcc gagggcggac gaggtgatct ggctgagggt cgtctcgtgg
     4381 ccctgtccgt ggggcgagga gccggtgacg acctgcacct tgcccgaggg gagcatgcgg
     4441 accgtggccg cctcccagcc gccggcgccg tagtccagct ggccgagcac ccgggacggg
     4501 gccaggccgc acatctcggt gaaggtggag atgccgatgc cgagctggat ccggtcaccg
     4561 tcggctcgac gctgcgcctg ctcggcacgc aggccgtcgt agtcgaacag ctccatcgcc
     4621 cgggcggtgg ccgcctcgta gttgcccgag tcgtaggtga gcccggcgac catctcgtag
     4681 gggaactcct cgtgggtgat ccagttcagc ttccggagct ccatcgggtc catgtcgagc
     4741 tcggccgcga gctcgtccat gatccgctcg atggcgaacg ttgcctccgg ccgcccggcg
     4801 ccccggtagg cgtcggtggg cgtcttggtc gtgaacacgc cggtgcagtt gaagcggtac
     4861 gcgtccatct tgtagatcgc cgggtacatg aaggccccga ggatgggcac gccgggggtc
     4921 acgagcatca ggtaggcgcc catgtcggcg agcaggtcga ccttcagtcc gagcagcttg
     4981 ccgtccttgg tggcggcgat ctcgatgtcc tggatctggc ctcggccgtg gtgcatcgtc
     5041 aggttcgtct cggaccgggt ctcggtccac ttcaccggcc ggcccaggcg cttggccacc
     5101 agcagggcga tgacctcctc gccggtcacc tgcagcttgg cgccgaaccc gccgccaacg
     5161 tcgggggcga tgacccggat cttgtgctcc ggcgtgccgg tgacgaccgc gagcatcgtg
     5221 cggaggatgt gggggatctg ggtggccgag tagatggtga agtcgcccga ggcggcctgc
     5281 gggatgcaga gcacggcgcg gggctcgatc ggcgtcggca gcaggcgctg ctggatgaac
     5341 cggcgcttca cgaccacgtc ggccttggcg aaggtctcgt ccacgtcggc gcccgtgggc
     5401 atcgagaaga agaacgcctt gttcgtgccc tcgtcctcgt ggaggaccac ctcgtcggtg
     5461 agggcctgct ccatgtcgac cacggcgggc agcggctcca ggtccacgag cacggtctcg
     5521 gcggcgtcga cggcgctggc ccggtcccgg gccacgacga ccgcgacggg gtcgccggcg
     5581 aagcggacct tgccgacggc gatgggcggg tgggcggggt gcaccatgtc cggtgtgacc
     5641 ggccaggcgc agggcagggt cgggcactcg tcggcgaagt cggcgcccgt gaacacgccg
     5701 accacgttcg gcatgccctc cgcctcggac gtgtcgatcg acccgatgcg ggcgtgggcc
     5761 atcgtgctgc gcacgaagac catgtggagc gtgcccaccg gggcgaggtt gtcggtccag
     5821 gtggtctggc ccgtgaccag gtgggcgtcc tccttgcgcc acggggcggt gcccacgccg
     5881 ccgacgtcgg ccgggcggtc gtcgatgacg gtcatgccgg cacctcctgg ctcgcgcccg
     5941 ccccttggcg gatctgcacg gcggcggccc gcaccgcctt cacgatgttc tggtagcccg
     6001 tgcagcggca gaggttgccc tcgagcccgt ggcggacctc gtcgtcggtc gggtcggggt
     6061 tctcctcgag caggtcgagg ctggccatga tcatgcccgg ggtgcagtag ccgcactgga
     6121 gggcgtgggc ttcgtggaac gcctgctgga gcgggtggag cgaaccctcg ccgtcccagg
     6181 tgtcggccag gccctggatg gtgagcacct cacgcccgtc ggcctggatg gccagcatcg
     6241 tgcaggactt caccgactga ccgtcgacgt gcaccgtgca cgcgccgcag ttggaggtgt
     6301 cgcaccccac gttggtgccg gtgagcccga gcgcctcgcg caggtggtgc acgagcagcg
     6361 tgcgcggctc gacgtcgtcg gagtaggccg tcccgtcgac ggtcacggtg atccttgcca
     6421 tgtgtccccc catgggctgc ggttcgtccg gggcgccggt gggcgctcct tgatggtgcg
     6481 ccggtggcgt cccctccacc ggcccgtcgc cactgaacca catccccgcg acgagcgctc
     6541 gtcgaccccc ggacgcgacg atcccccgcc gcgaggcggg ggaccggtcg gtcgttcggt
     6601 tgggggacct cagtgcgagg tcaggacggc gaggagacct cggtggtgtc gtcctcggag
     6661 tcgttcatgt cgacaccacg cagggccagg tcgatgagca ggtcgtcgaa gacctccttc
     6721 atctcgccgc ggtagcgcag ctggatctcg ttgctgtcgc ggtagcggcc gaagacgctg
     6781 ttggcctcaa gcacctcgag cccggcggag cgggagctgc gcacgcggtc ggaggcgagg
     6841 atgaacagat cggcgtggtc cgagtggacc gatgcgagct cctcgagcgc agcgaggacg
     6901 aggctctcct cgatggtgcc ggagaggcca ccacggtcgt cagcctcgag gccgagcgtc
     6961 ttggcggtcg tgagcacgtg catcagttcc tcctgtccgg aatcgtgttg ccacgatgcc
     7021 cgcgtacgtc ggtggacaca acctgccccg tgttgcagtg cagtgaatcc tgtcgcagcg
     7081 atctcctgcc cggagtaggt ctccaccgag ggtaggcctt cggcgccggt ctgctgcacc
     7141 accccggcgt cgcacccgcc acaccgccgt caggccatgg cgtggtggcc cccgtcgacc
     7201 tggaggatct cgccggtgac ggcgcgggcc aggtccgaca gcaggaagca gaccgcatcg
     7261 gccacgggcc ccgcatcgtg cgggtcccaa ccgagcggcg ctcgctgctc ccaggagtcg
     7321 aggaggaggt cgaagcccgg gatcgccgat gccgcccgcg tctccagcgg cccggccgcg
     7381 acgaggttcg accgtacgcg gcggggtccg agctccctcg ccaggtagcg gttcgccccc
     7441 tcgagcgcgc tcttgcagac gcccatccag ttgtaggtcg gccaggcacg ggacgcatcg
     7501 aagtcgaggc cgaccaggct ggccccggcg ggcggggcca ggtcggcgag cagctggccg
     7561 agctccacgt aggtgtggac gctcgtccgg aacgccagct cgaccgcctc cgcagggacg
     7621 cctgcgaccg agccgagcgc ggccttgggt gcgaacgcga tggcgtggag ggcgccgtcg
     7681 agcccgccga gctcgtcgcc cacccaggtg gccacggcct cgaggtcggt gggggaggtg
     7741 gcgtccatcg ccaggacggc cacgtcggcg gcgatgtcgg ccgcggcgtc ctgcgccccg
     7801 acgaggtccc gcggcatggc cgtggtcacg accgtggcac cgagctcgtg ggcccgcgcc
     7861 gccgtggcgt gggcgatgct gcctgcgttg gcgatgcccg tgaccaggat gcgcttgccg
     7921 tcgagcagcg gctggctcat cgaccgccgc catgacagag gaactggaag accggcatca
     7981 cagtgcgcac gttggccgtt gggctgggtc cctgtcaagg aacaccggca ttccgatgcc
     8041 ggtgacagcg tgctggtgcc gagtagcgtg ccggcgtggt ctccgagacg cccgggtgga
     8101 cgttcctgac caaccacggt cacgtcctgg tgtgcatcgc caacaacgca gaggtccgga
     8161 tcagcgagat cgccgaccag gtcggcatcg gtgagcgcgc tgcgcagggg atcgtgacgg
     8221 acctggtcga agggggctac gtgacccgca cccgggtcgg gcgccgcaac cactacgcgg
     8281 tccacaccag cctcccgttg cgccaccccc tcgagcggga ccacgtcgtg ggtgagctgc
     8341 tgcgggcagt ggcctcgccg gccgccgcgg acgactgagg ggcgcagtca gatcgccgtc
     8401 acgctccagg tggaggcgtc gacgagccgg ccgaggtcga ccgagaggcg cagcgtctcg
     8461 cccgccccga cctcctcgtc gaggcggaac atgcggttgc cccagctggt gttggtcgcc
     8521 aggggagagg tggtcagcgt cacctcgtcg tcgaagatcg cctcgaacca gacgcacgcg
     8581 ccgtcgacga tggcgtcggc accggccgtg cgctcgacga cgtgatgggt cggcagctgg
     8641 tcgaggccgt cgagcgtgtg gaggtcgagc tcgaggacgg gctggggcgt cccgatggtc
     8701 gcggcgacgg agccggggcg gacccagagc tgctcgttgc ggccggtgtc gaaccgcccg
     8761 gcgatcggcg agcgctccat cgccgccagg tcgatcccct cggggagctc gatgttccag
     8821 aagcggcgga cccgcatggc ctcgtgcatc gagatcggtt cgacgaagag ccggaaccgg
     8881 gcgggcagga tgcggccccc ggggcgcagg acccggtcgc gcaggtcgag cacgttctgg
     8941 accatgttct cgttgaacag ctcgtcgccc atctgctcgt gcaggacgag gtcgatcggc
     9001 tcgggtggcg tgaactcccg gctgttggcc tggacgaact cgatgttcgt gaagccgttg
     9061 tgcccggcga tctcgcgagc gacctcgatc acgtcgccgt gctcgacggc gtagaccttt
     9121 ccggccccgg cccggctggc caggaaggcg agcagcccgg tgccgctgcc gaggtcgagg
     9181 accacgtcac cgggggcgac gttgcgctgg atggcccggt ggtatgcctc gacccggacc
     9241 gagtcgctca gcatctcctc gtgctcggcg aggccaccga agttggtctc gttcatcagg
     9301 tcgtagacga ggtccatgag ccggtcgctc gccgccaccc gttccgccgc gaacccgagg
     9361 gccgaccggg cggccctggt cgccatctgg cgcgctcgct cccgcatgcg gcgaggctag
     9421 gggtgcgcac gtcgccaggc ggaaccggcg cacgaccggg ggcgctggac cgcaacgaaa
     9481 tcgcaacaaa gcgcgcacgc cggccgtggc gacgtcagat tcgcatcatc cgctgttcac
     9541 cgggcattgg cgggggaccc gttgacggac cccgtcgagg tccgtaacgt catcgacaca
     9601 tagttcgaac cgagcagggg gtcccgtcat ggcgcaccga acgcgagcag cggcagagca
     9661 gctggtgccc ggcaaccccg tcgaggtgcg cacgcgcttc gacggcacct gggttccggg
     9721 ataccgggtc tgcggcgagc gcagcgggcg ctacgtcatc gagcggtgca gcgacggctt
     9781 cgagctgccc gtggacttcg acctggccga gctgcggccg acggcgccga acccgggctg
     9841 agcggcccga ccggcccgtc tcagctgccg gggggcagga cctgaccggt ctggggatcc
     9901 caccggccga agtccgggtg cacctggatc tccgcggtgg tcgccagctc cagcaggtgc
     9961 tcctggaccc gctgctccga cggggtgagc tcggcgccct cctcgacctc gtcgggggtg
    10021 gtgtcggccg ggtcgtcgtc gccggccgcc tcgtcgaggg cgtcggtgat gttctggacg
    10081 gcagcgacgc cgcggagctc cagctggagc aggtcctcgg acaggccctg ctgctccatc
    10141 gccgcctgca ggccctccgg tccgccggcc tgctcctcga cctgggctcg cgccgcctcg
    10201 atgtcctcct cggtcacggg gacgtcgagc tcctcggcac ccgccaccag gacggaggac
    10261 tggatggcgc tgcccaggat ctgggccccc agctgggcgc gcgccgcgtc gccgtcctcg
    10321 ccctcgaact gctcggcgag ctgggggttg gcggcgaagt cctcgacgtg gccctcgatg
    10381 ctcgccgcgc tgatctcctc gccgtcgacc acggcggcga cgtcgtcgcc cagcgatccg
    10441 gcctcgccgg cgtcaccgga tgcaccggtc tcaccgtcct cggcagcctc ggcagtggtg
    10501 tccgtcgact cctggtcggc gcggtcctcg ctgtcggcgt cgtcaccgca tccggcgagg
    10561 aacgtgacgg cggccaggag ggccagggcg agcgtgcggg gg
//
LOCUS       DATISU010000058        10173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_747884, whole genome shotgun sequence.
ACCESSION   DATISU010000058 DATISU010000000
VERSION     DATISU010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 10173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10173
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747884"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1836)
                     /locus_tag="VLR27_02610"
     CDS             complement(<1..1836)
                     /locus_tag="VLR27_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HSP02363.1"
                     /translation="MSAAPPNPSTTATAGSSSVWGALEPHLPKVAKPARYIGLEDGAQ
                     ASERAPHKVGWLLCYPDTYEIGLPNQGLQILREIVNERDDAYAERGYAVWSDLEAIMR
                     RERIPFFSVETHTSAAEFDAIAFNLSAELTYTNVLNCVDLAGVPVRTDERDETHPLVV
                     VGGHCTYNPEPLADFVDIVVLGDGEEVIGEITDVLREWRAEGKRPGSRPNVLRALAGV
                     EGVYVPSLYDVAYDGPFLAEVTPRYPDVPERVEKRTVADLAEWPYPKQQLVPLTEVVH
                     DRLNVEVFRGCTRGCRFCQAGMITRPVRERPAEQVRSMIDAGLKRTGYDEVTLTSLST
                     ADFSGIEQTVQDVMDLDTGSAQPITLGLPSLRVDAFTVGLANKIQNARRTGLTFAPEA
                     GTWRMRQVINKLIREEDLYGAVGSAYSQGWRRMKLYFLTGLPSETDEDTLGIAELAKN
                     CVELGREHHKNPSVTVSVGGFVPKPFTPFQWFGQNTEEELRRKINLLKDATRKMGGVQ
                     LKWHDPRASVIEGLLSRGDRRLGRVLELVWRRGGVFQEWGEHFDEKLWLDAMAECGLD
                     PDWYIHRHRTEDEVLPWDHLSAGLHKDFLWMDWQDALAEVGLPDCR"
     gene            1907..3955
                     /locus_tag="VLR27_02615"
     CDS             1907..3955
                     /locus_tag="VLR27_02615"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HSP02364.1"
                     /translation="MANHLLTNGTWAVVVDTGGAVPAVQHWGADPGPAPAGHAALSDR
                     PLPPSTLDVDQALPVVAITTGDPGRPGLAGCRTDGSAAHPRFRAVSCGGDETALRFEA
                     VDDDAGLALAGELRLGPTLAVEVSVTNTGPTPYLLQRLAATLPLPADADEVLGFEGHW
                     CAEMTPTRSPWTTGTVAVENRRGRTSHDRVPVLFAGRRGFGELAGEVHGVHLAWSGNA
                     ELVLEHRTDGRRAVHAGELLTPGEVVLAPGDTYTSPTVHAVWGDGLNAASAGFHAEVL
                     AHVPERPRPVTLNTWEAVYFDHDAEVLRALADRAASVGVERFVLDDGWFGGRRDDTAG
                     LGDWWVSPDAHPDGLAPLIDHVRGLGMEFGIWVEPEMVNPDSDLYRDHPDWALGDPTV
                     LGRNQLVLDLSIDACWQHLFDALHRLLADHDIAYVKWDMNRDLAGAGAHHQVHALYRL
                     LDALGAAHPGVEIESCASGGGRADLGILRRTCRIWASDCNDALERQTIQRGWSYLLPP
                     AVTGAHIGPPRAHTTGRTQRLAFRAATALFGHLGIEWNLLDAGERHLEALTWWIDLHK
                     RLRPLLHGGDVVRLDPVDPSAHAHGVVATDRSEALFAYAQLTQSRRSAPPTLRLAGLD
                     PDRTYRVTHLAAPGEVLGWGRQPAWLAEPVEGTGRWLQTVGLQPPLLHPESALLIHLT
                     "
     gene            complement(3971..5128)
                     /gene="rodA"
                     /locus_tag="VLR27_02620"
     CDS             complement(3971..5128)
                     /gene="rodA"
                     /locus_tag="VLR27_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HSP02365.1"
                     /translation="MESFAGRWPGTTRAGTMRRNPAAPWRHVDLLLLGSVGLIALIGT
                     LMVFSSTRGTGATAANTAILERHLAFVATGVTVMALTTMADYRRFRDWAPLLYGAVCL
                     LLLGVVSPFGTEVNGAQAWFQIGPVQVQPSEFSKIAVIVGLASLGAHFGSDIDLRRLA
                     ILLAVPGIPLALIMLQPDPGTAMVFVAITMGMLLVTGVRAKHIAGLTLVGLTAVVGLL
                     SSGLLADYQKDRLTVFLNPAAGLQAEAYNLNQSKIAIGSGGVFGKGLFNGTQTQLDIV
                     PEQHTDFIFTAVGEELGFIGAATMLVLFAVVGWRIWRIAQLSRDQFGVLLCVGVLSML
                     AFQIFQNAGMTMGIMPITGIPLPFVSYGGSSTIALFAGLGLVQGVHMRRFT"
     gene            complement(5128..7185)
                     /gene="mrdA"
                     /locus_tag="VLR27_02625"
     CDS             complement(5128..7185)
                     /gene="mrdA"
                     /locus_tag="VLR27_02625"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HSP02366.1"
                     /translation="MSARVESPRLRLGVLGIVVIALFVPLFARLWYLQVLSSESFTEA
                     AEANRIRVVQVEAPRGRILDRDGTVLVDNRVSIVVTVDRVRFAELEDREAEQMLERLA
                     AELTRYGTPMTPEQLQDRVDDVRYSRYAPVPVAEDATEELKIYVEEHQELFPSVAVER
                     VAVRRYPFGTRAAHVIGSVGEINDEELEDRANKPKTYIRGNQIGKTGVERIYEDDLRG
                     MPGQTVFEVDAMGDPVRILEDLSKPPIPGDDVWLTIDIETQALSEDLLAQALADARER
                     RVSGDNLANRGNAGSVVVTDPRNGEVLAMASFPVFDPADFVNGISRTRWEFLNSEEAD
                     KPILNRAIQGEYAPGSTFKLFTGMAAMAQGLRTPEQVINDRGSFTIEECGGPGCTFRN
                     AGSKPYGNVDMRRAMTVSSDVYFYMVGADFWGARGRLGDEALQETVRLFGFDQETGVP
                     LPSEQDGRIPTPQQRKAQYEANPDLFLTGDWFTGDNVNMSIGQGDVAVTPLQLANGYA
                     TFANGGTRYAPNIALKITRSGSEDTIKRSFDPRINGRVEMDPAHRQALLDGLVGVTTL
                     EGGTATTTFADFPNDTFPVAGKTGTAQVDQKADTALFAAFGPANDPRYQVAIVLEESG
                     FGGTAAAPVARALFDVFAGVAPMPVIQPGGALVEPAPPEGGDELNEAPSDRLDDIAEG
                     GAD"
     gene            complement(7182..7700)
                     /gene="mreD"
                     /locus_tag="VLR27_02630"
     CDS             complement(7182..7700)
                     /gene="mreD"
                     /locus_tag="VLR27_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HSP02367.1"
                     /translation="MLRAARLTLVLVLVLTVQTTWLADARPFGATGDLLLLVAIAAGM
                     AAGPVRGATVGFIAGVAMDLVLLTPFGLSSLTYRAVGFVVGTVHDGVLRSAPWIPVLV
                     TFVASALGIVFLVMLGQLLGQQFRVPGLARIVLVTAVINTMLAFPALFVARWIEKAAP
                     DRMLQPSMRGLR"
     gene            complement(7700..8560)
                     /gene="mreC"
                     /locus_tag="VLR27_02635"
     CDS             complement(7700..8560)
                     /gene="mreC"
                     /locus_tag="VLR27_02635"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HSP02368.1"
                     /translation="MTGPRRSGRSRLTIFLLVLTSITVLTLDFRGVGFLGDARRAVGS
                     ALSPLQGAADFVASPFQNTWNGITGYDDLEAENEALRIQVEQLEGEAARNADVAAQYD
                     ELVSQVDIPWVGALSTATARVVQQPASSFSHVIGIDKGSGAGIRVGMPVVVGDGDSLG
                     GLVGRVVEVGEGQASVQLVTDPDFRVGVRLVTSQRFGTASGQGVGRDLRIDTGIEPGE
                     PDADLPSLEILTTSGIDDRSSFPASIPVGKVTRTRPANGGLTLEVFAEPLADLDQLSF
                     VSVVLTTPVS"
     gene            complement(8592..9632)
                     /locus_tag="VLR27_02640"
     CDS             complement(8592..9632)
                     /locus_tag="VLR27_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HSP02369.1"
                     /translation="MSENVFTSLMGRDMAVDLGTANTVIYVRGRGIVLNEPSVVAVNV
                     RDGRPLAVGAEAKRMIGRTPSHIQAIRPLKDGVISDFEICEKMLRYFIQKVHQRRWAK
                     PRMVICVPSGITGVEQRAVLEAAEFAGARKPAHIIEEPMAAAIGAGLPVQEPTGNMIV
                     DIGGGTTEVAVISLGGIVASQSVRVAGDELDEAIIQFIKKEYSLAVGERAAEEIKMGM
                     GSAWPLEEELYAEVRGRDLITGLPKTIVTSTEEIREAIDEPVTAIVDAVKVTLDKTPP
                     ELAADIMEKGIVLAGGGAFLHGLDARLEHETGMPIVIANDPLHAVAFGSGLALEEFDA
                     LKDVLFNNPPEH"
     gene            complement(9732..10142)
                     /gene="ndk"
                     /locus_tag="VLR27_02645"
     CDS             complement(9732..10142)
                     /gene="ndk"
                     /locus_tag="VLR27_02645"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HSP02370.1"
                     /translation="MNRTFVMCKPDAVERGLVGEIVARFERKGLTLVAAELKTADRAL
                     AEAHYDEHRDKPFFGELVDFLTRSPVLAMVVEGPADNTFSICRTLIGKTSVDDAQPGS
                     IRGDFATTTNENLVHGSDSHESAQREIDLWFPSL"
BASE COUNT         1454 a         3521 c         3745 g         1453 t
ORIGIN      
        1 gcggcagtcg ggcaggccga cctcggccag cgcgtcctgc cagtccatcc agaggaagtc
       61 cttgtgcagg ccggcggaga ggtggtccca gggcaggacc tcgtcctcgg tgcggtgccg
      121 gtggatgtac cagtcggggt ccaggccgca ctcggccatg gcgtcgagcc agagcttctc
      181 gtcgaagtgc tcgccccact cctggaacac gccgccgcgg cgccacacca gctcgagcac
      241 ccggccgagg cgtcggtcgc cacgggacag caggccctcg atgaccgagg cccgcgggtc
      301 gtgccacttg agctgcacgc cacccatctt gcgggtggcg tccttgagca ggttgatctt
      361 gcggcgcagc tcttcctcgg tgttctgccc gaaccactgg aacggggtga acggcttcgg
      421 gacgaacccg cccacgctga ccgtcacgct cgggttcttg tggtgctcgc ggccgagctc
      481 gacgcagttc ttggccagct cggcgatgcc gagggtgtcc tcgtcggtct cgctgggcag
      541 gccggtgagg aagtacagct tcatgcggcg ccagccctgc gagtaggccg agcccaccgc
      601 gccgtacagg tcctcttcgc ggatgagctt gttgatgacc tggcgcatgc gccacgtgcc
      661 ggcctcgggg gcgaaggtga ggccggtgcg ccgggcgttc tggatcttgt tggccagccc
      721 gacggtgaac gcgtcgaccc gcagcgacgg gagcccgagg gtgatgggct gggcgctgcc
      781 ggtgtcgagg tccatcacgt cctggacggt ctgctcgatg cccgagaagt ccgccgtgga
      841 cagactggtg agggtgacct cgtcgtagcc ggtgcgcttc aggccggcgt cgatcatcga
      901 gcggacctgc tcggcggggc gctcgcgcac gggccgggtg atcatcccgg cctggcagaa
      961 ccggcagccc cgcgtgcagc cgcggaagac ctcgacgttg aggcggtcgt ggaccacctc
     1021 ggtgagcggg accagctgct gcttggggta cggccactcg gcgaggtcgg cgacggtgcg
     1081 cttctcgacc cgctcgggca cgtccgggta gcggggggtg acctcggcga ggaacgggcc
     1141 gtcgtaggcc acgtcgtaca gcgagggcac gtacacgccc tcgacgccgg cgagggcccg
     1201 cagcacgttg ggccgggatc cggggcgctt gccctcggcc cgccactcgc gcagcacgtc
     1261 ggtgatctcc ccgatgacct cctcgccgtc gcccaggacg acgatgtcga cgaagtcggc
     1321 gagcggttcg gggttgtagg tgcagtggcc gcccacgacg accagcgggt gggtctcgtc
     1381 gcgctcgtcg gtgcggaccg gcacgccggc caggtccacg cagttgagga cgttggtgta
     1441 ggtgagctcg gcggagaggt tgaacgcgat ggcgtcgaac tcggcggcgc tggtgtgggt
     1501 ctcgaccgag aagaacggga tgcgctcgcg gcgcatgatc gcctcgaggt cgctccacac
     1561 cgcgtagccg cgctcggcgt aggcgtcgtc gcgctcgttg acgatctcgc gcaggatctg
     1621 caggccctgg ttcggcagcc cgatctcgta ggtgtccggg tagcagagca gccagcccac
     1681 cttgtggggt gcccgctccg aggcctgcgc accgtcctcg aggccgatgt accgggccgg
     1741 cttggccacc ttgggcaggt gcggctcgag cgccccccag acgctggagg accccgcggt
     1801 ggctgtcgtg gaggggttcg ggggcgcggc ggacacggtc cgcccaggct accggacacc
     1861 ctgcagcgtt ccccgatcgc cccggcggac gtggcagcgt gcggaggtgg cgaaccacct
     1921 gctcacgaac gggacctggg ccgtcgtcgt cgacaccggc ggggcggtgc cggcggtgca
     1981 gcactggggt gcggatcccg gtccggctcc cgccggccat gcggcgctgt cggaccgccc
     2041 gctgccaccg tccaccctcg acgtcgacca ggcgctgccc gtggtggcga tcaccaccgg
     2101 cgatcccggc cggcccgggc tcgccgggtg ccggaccgac gggagcgccg cccacccccg
     2161 gttccgagcc gtgtcctgcg gcggcgacga gacggcgctc cggttcgagg cggtcgacga
     2221 cgacgccggc ctggcgctgg ccggcgagct ccggctcggc ccgaccctgg ccgtcgaggt
     2281 ctcggtcacc aacaccggtc cgacgccgta cctgctgcag cgcctggcgg ccacgctccc
     2341 cctccccgcc gacgccgacg aggtgctggg gttcgagggc cactggtgcg ccgagatgac
     2401 accgacccgc agcccgtgga ccaccggcac cgtggcggtc gagaaccggc gcggccggac
     2461 ctcccacgac cgcgtcccgg tgctcttcgc cggacggcgc ggcttcggcg agctggccgg
     2521 cgaggtccat ggggtccacc tggcgtggag cggcaacgcc gagctggtcc tcgagcaccg
     2581 caccgacgga cggcgcgccg tgcacgccgg cgagctgctc acgcccggcg aggtcgtcct
     2641 cgcgccgggc gacacctaca cctcgcccac cgtgcacgcc gtctggggcg acgggctcaa
     2701 cgccgccagc gccggcttcc acgccgaggt gctggcccac gtgcccgagc ggccccgccc
     2761 ggtgaccctc aacacctggg aggccgtgta cttcgaccac gacgccgagg tcctgcgggc
     2821 gctggccgac cgggctgcct ccgtgggcgt ggagcgcttc gtgctcgacg acggctggtt
     2881 cggcggtcgc cgggacgaca ccgccggact cggcgactgg tgggtgtcgc ccgacgccca
     2941 ccccgacggc ctcgcgccgc tcatcgacca cgtccggggt ctcggcatgg agttcggcat
     3001 ctgggtggag cccgagatgg tgaaccccga cagcgacctc taccgcgacc atccggactg
     3061 ggcgctcggc gacccgaccg tcctcggccg caaccagctg gtgctggacc tgtcgatcga
     3121 cgcctgctgg cagcacctgt tcgacgccct gcaccggctg ctcgccgacc acgacatcgc
     3181 ctacgtgaag tgggacatga accgcgacct ggccggcgcc ggcgcccacc accaagtcca
     3241 cgccctgtac cggctgctcg acgccctcgg cgccgcccac cccggtgtgg agatcgagtc
     3301 gtgcgcctca ggcggtggcc gggccgacct cggcatcctc cggcgcacct gccggatctg
     3361 ggcgtctgac tgcaacgacg cgctcgagcg ccagacgatc cagcggggat ggtcgtacct
     3421 gctgccgccg gcggtcaccg gcgcgcacat cggcccgccc cgggcgcaca cgacgggccg
     3481 gacccagcgg ctggcgttcc gggccgccac cgccctgttc gggcacctcg gcatcgagtg
     3541 gaacctgctc gacgccggcg aacgccacct cgaggccctg acgtggtgga tcgacctcca
     3601 caagcgcctg cgcccgctgc tgcacggcgg cgacgtggtc cgcctcgacc ccgtcgatcc
     3661 gtccgcccac gcccacggcg tcgtcgccac cgatcggtcc gaggccctgt tcgcctacgc
     3721 ccagctcacg cagtcccggc gatccgctcc ccccacgctg cggctggccg gtctcgaccc
     3781 ggaccgcacc taccgggtca cccacctggc cgcgccaggc gaggtcctcg ggtggggccg
     3841 ccaaccggcc tggctggccg agccggtcga ggggacgggg cgttggttgc agaccgtcgg
     3901 cctccaaccc ccgctgctcc accccgagtc cgcgctcctg atccacctga cctgaggcga
     3961 cgcggacggg tcaggtgaag cggcgcatgt gcacgccctg caccaacccg aggccggcga
     4021 agagcgcgat ggtcgacgaa cccccgtagc tcacgaaggg cagcgggatg ccggtgatgg
     4081 gcatgatgcc catcgtcatg ccggcgttct ggaagatctg gaaggccagc atcgacagca
     4141 cgccgacgca cagcagcacg ccgaactggt cccgcgacag ctgggcgatc cgccagatcc
     4201 gccagccgac gacggcgaac aggacgagca tggtggccgc cccgatgaac ccgagctcct
     4261 cccccaccgc ggtgaagatg aagtcggtgt gctgctcggg gacgatgtcg agctgggtct
     4321 gggtgccgtt gaacagcccc ttgccgaaga ccccaccgga gccgatggcg atcttggact
     4381 ggttcaggtt gtaggcctcg gcctgcagcc cggccgccgg gttcaggaac accgtcaggc
     4441 gatccttctg gtagtcggcc agcaggcccg agctgagcag accgaccacc gcggtgagcc
     4501 cgaccagcgt gagcccggcg atgtgcttcg cccgcacgcc cgtcaccagc agcatgccca
     4561 tggtgatggc gacgaagacc atggcggtgc ccgggtcggg ctggagcatg atcagcgcca
     4621 gggggatgcc cggcacggcc aggaggatgg cgaggcggcg cagatcgatg tcgctgccga
     4681 agtgcgcgcc gagggacgcc agcccgacga tcacggcgat cttggagaac tcggagggct
     4741 gcacctggac cggaccgatc tggaaccagg cctgtgcgcc gttcacctcg gtgccgaacg
     4801 gcgacaccac gccgagcagg aggaggcaca ccgccccgta cagcagcgga gcccagtccc
     4861 ggaaccgccg gtagtcggcc atcgtcgtga gcgccatcac ggtgacgccg gtggccacga
     4921 aggcgaggtg ccgttccagg atcgcggtgt tcgccgcggt cgcgcccgtg cccctcgtcg
     4981 acgagaagac catgagcgtg ccgatcagcg cgatgaggcc gaccgagccc aggagcagca
     5041 ggtcgacgtg acgccagggc gcggccgggt tccggcgcat cgtcccggcc cgggtggtgc
     5101 cgggccagcg gccggcgaag ctctccatca gtccgcaccc ccctcagcga tgtcatcgag
     5161 ccggtccgac ggcgcctcgt tgagctcgtc gccaccctcc ggcggggcgg gctcgaccag
     5221 cgccccgccg ggctggatga cgggcatcgg cgcgacgccg gcgaagacgt cgaacagggc
     5281 acgggccacc ggggccgctg ccgtgccgcc gaacccggac tcctcgagca cgatcgccac
     5341 ctggtaccgg gggtcgttgg ccggcccgaa ggcggcgaag agggcggtgt cggccttctg
     5401 gtcgacctgg gcggtgccgg tcttgccggc gaccgggaag gtgtcgttcg ggaagtcggc
     5461 gaaggtggtg gtggccgtgc cgccctcgag cgtggtcacg ccgacgaggc cgtcgagcag
     5521 cgcctggcgg tgcgcgggat ccatctcgac ccgaccgttg atccggggat cgaagctgcg
     5581 cttgatcgtg tcctccgacc cgctccgggt gatcttgagg gcgatgttcg gcgcgtagcg
     5641 ggtgccgccg ttggcgaagg tggcgtaccc gttggcgagc tggagcgggg tgacggcgac
     5701 gtcgccctga ccgatcgaca tgttcacgtt gtcgccggtg aaccagtcgc cggtgaggaa
     5761 cagatcgggg ttcgcttcgt actgggcctt gcgctgctgc ggcgtgggga tgcgaccgtc
     5821 ctgctcggag ggcaggggca ccccggtctc ctggtcgaag ccgaacaggc gcacggtctc
     5881 ctgcagcgcc tcgtcgccga gtcgaccccg ggcgccccag aagtcggcac cgaccatgta
     5941 gaagtacacg tccgacgaca cggtcatggc ccgccgcatg tcgacgttgc cgtagggctt
     6001 cgagccggcg ttgcggaagg tgcagccggg tccgccgcac tcctcgatgg tgaaggaccc
     6061 gcggtcgttg atgacctgct cgggcgtgcg gaggccctgg gccatggccg ccatgccggt
     6121 gaagagcttg aaggtggagc ccggggcgta ctcgccctgg atcgcccggt tcaggatcgg
     6181 cttgtccgcc tcctcgctgt tgaggaactc ccagcgcgtc cgcgagatgc cgttgacgaa
     6241 gtcggccggg tcgaagacgg ggaagctggc catggcgagc acctcgccgt tgcgcgggtc
     6301 ggtgaccacc accgagccgg cgttgccccg gttcgcgagg ttgtcgccgg acacccgccg
     6361 ctcccgggca tcggccagcg cctgggcgag caggtcctcg gacagggcct gggtctcgat
     6421 gtcgatcgtc agccacacgt cgtcgccggg gatgggcggc ttcgagaggt cctcgaggat
     6481 ccggaccgga tcgcccatcg cgtcgacctc gaacacggtc tggccgggca tgccccgcag
     6541 gtcgtcctcg tagatccgct ccacgccggt cttcccgatc tggttgccgc ggatgtaggt
     6601 cttgggcttg ttggcccggt cctcgagctc ctcgtcgttg atctccccca ccgacccgat
     6661 gacgtgggca gcgcgggtgc cgaacgggta gcggcgcacg gcgacgcgct cgaccgccac
     6721 ggaggggaac agctcctggt gctcctcgac gtagatcttg agctcctcgg tggcgtcctc
     6781 ggccaccggc accggcgcgt agcgcgagta ccggacgtcg tcgacgcgat cctgcagctg
     6841 ctccggggtc atcggcgtgc cgtagcgcgt gagctcggcg gccagccgct cgagcatctg
     6901 ctcggcctcg cggtcctcga gctcggcgaa gcggacgcga tcgacggtga cgacgatgga
     6961 cacccggttg tcgaccagga cggtgccgtc ccggtcgagg atgcggccgc ggggcgcctc
     7021 gacctgcacg acgcggatcc ggttggcctc agcggcctcc gtgaagctct ccgaggacag
     7081 gacctgcagg taccagagcc gggcgaacag cggcacgaac agggcgatga ccacgatgcc
     7141 gagcacgccg aggcgcaggc ggggcgattc gacgcgggcg ctcatcgcag accccgcatc
     7201 gacggctgga gcatgcggtc gggcgcggcc ttctcgatcc agcgggccac gaagagggcg
     7261 gggaacgcca gcatggtgtt gatgacggcg gtgaccagga cgatccgggc caggcccggc
     7321 acccggaact gctggccgag cagctggccc agcatcacga ggaagacgat cccgagggcg
     7381 gaggcgacga aggtgacgag caccgggatc cagggcgcgg agcgcaggac cccgtcgtgg
     7441 acggtgccga ccacgaaccc caccgcccgg taggtgagcg acgagaggcc gaagggggtc
     7501 agcagcacga ggtccatcgc cacaccggcg atgaagccga ccgtggcgcc ccgcaccggc
     7561 ccggcggcca tgccggcggc gatggcgacc agcaggagca ggtcgccggt ggcgccgaac
     7621 gggcgggcgt cggcgagcca ggtcgtctgg acggtgagga ccaggaccag gacgagggtg
     7681 aggcgggcgg cgcgcagcat cagctgaccg gggtggtgag cacgacggag acgaagctca
     7741 gctggtccag gtcggccagc ggctcggcga agacctcgag ggtgaggcct ccgttggccg
     7801 gccgggtgcg ggtgaccttg cccacgggga tggaggcggg gaacgacgac cggtcgtcga
     7861 tgccggaggt ggtgaggatc tcgagcgacg ggaggtcggc gtcgggctcg cccggctcga
     7921 tgccggtgtc gatgcgcaga tcccgcccga cgccctggcc cgaggcggtg ccgaagcgct
     7981 ggctggtgac gaggcgcacg cccacccgga agtcggggtc cgtcaccagc tgcacggagg
     8041 cctgcccctc gccgacctcg acgacgcgac cgacgaggcc accgaggctg tcaccgtcgc
     8101 ccaccaccac gggcatgccg acccggatcc ccgcgccgga gcccttgtcg atgccgatga
     8161 cgtgggagaa cgagctggcc ggctgctgca cgacccgggc ggtggcggtg ctgagcgccc
     8221 ccacccaggg gatgtcgacc tggctcacga gctcgtcgta ctgggcggcg acatcggcgt
     8281 tgcgggccgc ctcgccctcg agctgctcca cctggatgcg cagggcttcg ttctccgcct
     8341 cgaggtcgtc gtagccggtg atcccgttcc aggtgttctg gaagggcgag gccacgaagt
     8401 cagccgctcc ctgcaggggg ctgagcgcag atcccaccgc ccggcgggcg tcgccgagga
     8461 agccgacgcc ccggaagtcc agggtgagca cggtgatcga ggtgagcacg agcaggaaga
     8521 tggtcagccg tgaccgtccg ctgcggcggg gaccggtcat ggccatgaga ggccctcaga
     8581 catggcgggg atcagtgttc gggcgggttg ttgaacagca cgtccttcag ggcgtcgaac
     8641 tcctcgaggg cgaggccgga cccgaaggcg acggcgtgca gcggatcgtt ggcgatcacg
     8701 atcggcatgc cggtctcgtg ctccagacgg gcatcgaggc cgtggaggaa cgcgccgccg
     8761 ccggcgagca cgatcccctt ctccatgatg tcggccgcca gctccggcgg cgtcttgtcg
     8821 agggtgacct tgacggcgtc gacgatggcc gtcaccggct cgtcgatggc ctcgcggatc
     8881 tcctcggtgg aggtgacgat cgtcttgggg agcccggtga tgaggtcgcg gccgcgcacc
     8941 tcggcgtaga gctcctcctc gagcggccag gccgagccca tgcccatctt gatctcctcg
     9001 gcggcccgct cgcccaccgc gagggagtac tccttcttga tgaactggat gatggcctcg
     9061 tccagctcgt cgccggcgac gcgcaccgac tggctggcca cgatgccgcc gagggagatg
     9121 accgcgacct cggtggtgcc gccgccgatg tcgacgatca tgttgccggt gggctcctgg
     9181 acgggcagcc cggcgccgat ggccgcggcc atgggctcct cgatgatgtg cgcgggcttg
     9241 cgggcgccgg cgaactcggc ggcctcgagc acggcccgct gctccacgcc ggtgatgccc
     9301 gacggcacgc agatgaccat gcgcggcttg gcccagcggc gctggtgcac cttctggatg
     9361 aagtaccgca gcatcttctc gcagatctcg aagtccgaga tcacgccgtc cttgagcggg
     9421 cggatggcct ggatgtgcga gggcgtgcgg ccgatcatcc gcttggcctc tgccccgacg
     9481 gcgaggggtc ggccgtcgcg cacgttgacg gcgaccaccg agggctcgtt gagcacgatg
     9541 ccacgcccgc ggacgtagat caccgtgttg gcggtgccga ggtcgaccgc catgtcgcgg
     9601 cccatcaggg aggtgaagac gttctcggac atgcgttcac gattccgtcg gggacctggt
     9661 ccgggcgacc ggtcagcgta gcggaggggc ccaggctaac tctctgtcac cgaaccgcaa
     9721 cagtcagcgg ctcagagcga tgggaaccag aggtcgatct cgcgctgggc ggactcgtgg
     9781 gagtcgctgc cgtggaccag gttctcgttg gtcgtggtgg cgaagtcgcc gcggatcgag
     9841 ccgggctggg cgtcgtcgac gctggtcttg ccgatcaggg tgcgacagat cgagaaggtg
     9901 ttgtcggccg ggccctcgac caccatggcc agcaccgggc tgcgggtgag gaagtcgacc
     9961 agctcgccga agaacggctt gtcgcggtgc tcgtcgtagt gggcttcggc gagggcccga
    10021 tcggcggtct tgagctcggc ggcgacgagg gtgagcccct tgcgctcgaa gcgggcgacg
    10081 atctccccga cgaggccgcg ctcgacggca tcgggcttgc acatgacgaa ggtccggttc
    10141 acggggcggg acggtagcgg ccgtcagcgg cga
//
LOCUS       DATISU010000059         6143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_701595, whole genome shotgun sequence.
ACCESSION   DATISU010000059 DATISU010000000
VERSION     DATISU010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701595"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..992)
                     /locus_tag="VLR27_02650"
     CDS             complement(156..992)
                     /locus_tag="VLR27_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HSP02371.1"
                     /translation="MAGVRIVTDSACDLTDDEATSLGIEVVPLSIRFGSDEYEDRTEL
                     SAGDFYEKMDQRSELPETAAPSPGKFEAAFRRQAEAGADAVVCINLSSDLSATMAAAE
                     NAARAIGDDLDVRVVDSKSITAGLGTQVLLAAQRAADGASADEIVALIEDLRARTHVI
                     GCLDTLENLKKGGRIGGAQAMLGSLLSIKPMIDISTGVVEEAGKHRTRKKSLEALREK
                     VRAAGEVEHLAIMHGMAPDVGVMTGLLDEDGYDYRLGHIGAVIGSHGGPRVVGACWVD
                     PA"
     gene            complement(1061..3049)
                     /locus_tag="VLR27_02655"
     CDS             complement(1061..3049)
                     /locus_tag="VLR27_02655"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HSP02372.1"
                     /translation="MTRGRRLLLAIAAAVAALVSVAPPVPAQTDPPPPAGIELADRSP
                     WVAPEGEATFLLDAVGDLTGATIQVEVFSALDSVEELDESATEDVGVRLAFAPPVGIE
                     TLPPGPDGSRIVGLRVSADPSDGQTVQLVAPGVHPVVITLLGADGTVLDEIRTPLVRL
                     GDETDDWRAPDLAVLLDVAAAPTLQPDGTRAIEPGELRRLARVGDLLAAHPDLDLSVA
                     AVPDTVDALGTLPDAAAATLLDRLRGRDLLAMPYLPLPVAALLEGQLGGMVAPLADRG
                     DALLADRLDATPERDLWVGTTAVGRAGAELLDDLGYGSIIAEPADDTDDDEPAPLIDA
                     GPRTSPATRPLTAVVTDPVLSAAVARPLDDEADAAHLALARLLLRTVDADRDDEDDDP
                     GTVLVRPGELASESVLAGLLGLLDAPEAPVRVGGVGLVDGDPDEDVEPLRRREAPSPD
                     LADVADRVVATAVPLDTFQTLIGPQSSRADDLRLQVATAIATTTPPDRRDAAMGVVED
                     VLGTAFGRVRLSGQTDLNLTSRRGTLPVTIENANPFPVEAVVRTRSDRLAFPDGGETP
                     VTVDGGDILRIDVTVEALATGSVPVFVELWTPDGGVRLDGRQLNVRSTAISGVGLVLS
                     LGALAVLVVWWVRNWRRTRHERTLAGAAGSGTAPEPMG"
     gene            complement(3046..3795)
                     /locus_tag="VLR27_02660"
     CDS             complement(3046..3795)
                     /locus_tag="VLR27_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP02373.1"
                     /translation="MTATDTVTARGVRMRVRPWRGRSDTAELSAVPAGAMVPPGLVAM
                     AVERARTRGYAAVVTPALPAGEWRPYLDAGFTVREELHLLAHDLLDLPARRTAERAVR
                     TRRVLRRDRDRVLAIDQAAFDPFWRLDADALEDAVRATPSTRFRLDREGRGYALTGRA
                     GDRGYVQRLAVDPAEQGSGLGSALVVDSLWWLRRWRVREALVNTQTSNGRAVALYERL
                     GFRQRSEGLAVLELDVASHPAPPTPDPGPVP"
     gene            3892..4905
                     /locus_tag="VLR27_02665"
     CDS             3892..4905
                     /locus_tag="VLR27_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HSP02374.1"
                     /translation="MATLLYTDPRFVEHDTTAGHPERPERMDAVGRGVAASVVAEDLV
                     HLTPREATVDELALVHDRSYLDALERFCASGGGHLDPDTVAAARSWDIARLAAGAGLD
                     AIDRLKAGEADAAFLAVRPPGHHATPSRAMGFCLLNNVAVAAAALADAGERVLVVDID
                     AHHGNGTQDAFWDDPRVTYLSVHEHPMYPGTGMVRETGGPNAPGTTINLPVPSATSGD
                     VYRRVVDDVIVPVAERMAVTWLLLSTGFDAHRNDPLTDLGLSSGDYYDLTKRILALVP
                     AGRRLAFLEGGYDLDAITTSTAATLAALAGVEHRPEPVTTDAADAPGHQAVGAARLIH
                     QLA"
     gene            4929..6038
                     /locus_tag="VLR27_02670"
     CDS             4929..6038
                     /locus_tag="VLR27_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HSP02375.1"
                     /translation="MTDTTEHVDDQPVDHVALLRALLERGGSDLHCKPGSPPALRVGG
                     ALLREPDDPVLGQRLTAPAVAMLAQTLLTTDQQQVLLQDGSVVATHAIPEVGRFRVAV
                     YRQRGSVAVVVHAVPQQVLRLEELGLPDSVSSLASAERGLVLVASPVGNGATTTLAAM
                     VDHVNRTRACHVVTVEDPAEVLHRDDRSLISQLEVPTDVAGLAEGVRAASRLDADVIT
                     ISDIADRDVALAALDGVARGRLVLATIGGNTVRSVIDGFLELFTFEERPNVRQTLARS
                     LTGVVVQRLLTGTDGRLLPVAEVLVATAKIQEALAEESRMGELGALLADGVFHGMQTL
                     DQSLRDQVRGGRISEATALGAATDPEELRIELLRS"
BASE COUNT          770 a         2258 c         2361 g          754 t
ORIGIN      
        1 gagctggtag cggccgccga tggtggtgcc ctgctcgagg ggactgccgt tcggggtcac
       61 ggtcgtcggc cgcgtggggt gctccgacac cccaccgacg ctacccgtcg gggctcaccg
      121 ggccgcgagt cgccggcgcg ggcagggggg tcggctcagg ccgggtcgac ccagcaggcg
      181 ccgacgacgc gggggccgcc gtgggagccg atgaccgcgc cgatgtggcc cagccggtag
      241 tcgtagccgt cctcgtcgag caggccggtc atgaccccga cgtcgggcgc catgccgtgc
      301 atgatcgcca ggtgctcgac ctcgccggcg gcgcggacct tctcgcgcag cgcctcgagg
      361 gacttcttgc gcgtgcggtg cttgcccgcc tcctcgacca caccggtgga gatgtcgatc
      421 atcggcttga tcgacagcag cgaaccgagc atggcctgcg cgccaccgat gcggccgccc
      481 ttcttgaggt tctcgagcgt gtcgaggcag ccgatgacgt gcgtgcgggc ccgcaaatcc
      541 tcgatgaggg ccacgatctc gtcggcggag gcgccgtcgg cggcccgctg ggcggcgagc
      601 agcacctggg tgccgaggcc ggcggtgatg gacttggagt ccacgacccg gacgtcgagg
      661 tcgtcgccga tggcgcgggc ggcgttctcg gccgcggcca tggtggccga caggtcggac
      721 gagaggttga tgcacaccac ggcgtcggcg ccggcctcgg cctggcggcg gaaggcggcc
      781 tcgaacttgc cgggggaggg cgcggcggtc tcgggcagct cgctgcgctg gtccatcttc
      841 tcgtagaagt ccccggccga cagctcggtg cggtcctcgt actcgtcgct cccgaagcgg
      901 atcgacagcg gcaccacctc gatgccgagg gaggtcgcct cgtcgtcggt caggtcgcag
      961 gcgctgtcgg tgacgatgcg gacgccagcc atggtgctct ccggtggtgc tcggtcggtc
     1021 gggacgccgc ggggcgcctg gccccccggt gccggagtgt ctagcccatc ggctccggag
     1081 ccgtccccga cccggcggcc ccggcgagcg tccgctcgtg ccgggtccgg cgccagttcc
     1141 gcacccacca cacgaccagc acggcgaggg cgcccagcga cagcaccagg ccgacgccgc
     1201 tgatcgcggt ggaccggacg ttgagctggc gaccgtcgag gcggacgccg ccgtcggggg
     1261 tccagagctc cacgaacacc ggcaccgacc cggtggcgag ggcctccacc gtcacgtcga
     1321 tccgcaggat gtcgccgcca tcgacggtca cgggcgtctc cccgccgtcg gggaacgcga
     1381 gccggtcgct gcgggtccgc accacggcct cgaccgggaa ggggttggcg ttctcgatgg
     1441 tgacggggag cgtgccgcga cgggacgtga ggttcaggtc ggtctggccg gagaggcgga
     1501 cgcgaccgaa ggcggtgccg aggacgtcct ccaccacgcc catcgctgcg tcgcgccggt
     1561 ccggcggcgt ggtggtggcg atggcggtgg cgacctgcag gcggaggtcg tcggcccgcg
     1621 aggactgcgg gccgatcagg gtctggaacg tgtcgagcgg gacggccgtg gcgacgaccc
     1681 ggtcggcgac atcggcgagg tccgggctcg gcgcctcccg ccggcgcagc ggctcgacgt
     1741 cctcgtcggg atcgccgtcg acgagcccga caccacccac gcgcaccggg gcctccggcg
     1801 cgtcgagcag ccccagcagg cccgccagca cggactccga cgccagctcg ccgggccgga
     1861 cgaggacggt gcccgggtcg tcgtcctcgt cgtcgcggtc tgcgtcgacc gtacggagca
     1921 gcaaccgggc cagggccagg tgggcggcgt cggcttcgtc gtcgaggggc cgggcgacgg
     1981 cggccgacag caccggatcg gtcacgaccg cggtgagggg acgggtggcc ggtgacgttc
     2041 gcggtccggc gtcgatcagc ggcgcgggtt cgtcgtcgtc ggtgtcgtcg gccggttcgg
     2101 cgatgatcga cccgtagccg aggtcgtcga gcagctcggc gcccgctcgg ccgaccgcag
     2161 tcgtgcccac ccacaggtcg cgctcgggcg tggcgtcgag ccggtcggcc agcagggcat
     2221 cgccgcggtc ggccagcggg gcgaccatcc cgccgagctg gccctcgagc agcgcagcca
     2281 ccggcagggg caggtagggc atcgccagga gatcccgtcc tcgcagccgg tcgaggagcg
     2341 tggccgccgc cgcatccggg agcgtgccga gcgcgtcgac ggtgtcgggc acggcggcga
     2401 ccgacaggtc gaggtcgggg tgcgcggcga ggaggtcccc cacccgggcg aggcgtcgca
     2461 gctcgcccgg ctcgatggcg cgggtgccat ccggttgcag cgtcggggcg gcggccacgt
     2521 ccaggaggac ggcgaggtcc ggggcccgcc agtcgtcggt ctcgtcgccg aggcgcacga
     2581 ggggggtgcg gatctcgtcg aggacggtgc cgtccgcgcc gaggagggtg atgacgacgg
     2641 ggtgcacgcc cggggcgacg agctgcaccg tctggccgtc ggagggatcg gccgacaccc
     2701 gcaggccgac gatccggctg ccgtcgggcc cgggcgggag cgtctcgatg ccgaccggtg
     2761 gcgcgaaggc gaggcggacg ccgacgtcct cggtggccga ctcgtcgagc tcctccaccg
     2821 agtccagcgc cgagaacacc tcgacctgga tcgtggcgcc ggtgaggtcc ccgaccgcgt
     2881 cgaggaggaa ggtggcctcg ccctccggtg cgacccaggg ggagcggtcg gccagctcga
     2941 tcccggccgg gggcggggga tcggtctggg ccgggaccgg cggtgcgacc gacaccagag
     3001 cggcgacggc agcggcgatg gcgagcagga ggcggcgccc gcgcgtcatg gcaccggtcc
     3061 ggggtccggg gtcgggggcg cggggtgcga ggccacgtcc agctccagca cggcgaggcc
     3121 ctcgctgcgc tggcggaacc cgagtcgctc gtacagcgcg acggcgcgcc cgttgctggt
     3181 ctgggtgttg accagcgcct cccgcacccg ccagcggcgc agccaccaca ggctgtcgac
     3241 gacgagggcg gacccgaggc ccgacccctg ctcggccgga tcgaccgcca ggcgctggac
     3301 gtagccgcgg tcgccggccc gcccggtgag ggcgtacccg cggccctcgc ggtcgagccg
     3361 gaagcgggtg gacggcgtgg cgcggaccgc gtcctcgagg gcatcggcgt cgaggcgcca
     3421 gaacgggtcg aaggcggcct ggtcgatggc cagcacgcgg tcccggtcgc ggcgcagcac
     3481 ccggcgggtg cggacggcgc gctcggcggt ccggcgggcg gggaggtcga gcaggtcgtg
     3541 ggcgagcagg tggagctcct cgcgcacggt gaagccggcg tcgaggtacg ggcgccactc
     3601 acccgccggg agcgccgggg tgacgaccgc ggcgtagccg cgggtgcggg cccgctcgac
     3661 ggccatggca accaggccgg gcgggaccat cgcccccgcc ggcaccgccg acagctcggc
     3721 cgtgtcggac cggccccgcc acggccgcac ccgcatgcgc acgccccggg cggtgacggt
     3781 gtcggtggcg gtcaccgttc cacgttacgg ccgggacccg ggcgggggga gcacggcggg
     3841 gtcccgcctc cgggcatcgg ggacggacgc cgatctgggc agactgtggc gatggccacg
     3901 ctgctctaca ccgatccgcg cttcgtcgag cacgacacca cggccggcca cccggagcgc
     3961 ccggagcgga tggacgccgt cggccgtggc gtggcggcgt cggtggtggc cgaggacctg
     4021 gtgcacctga cgccgcgcga ggcgacggtg gacgagctcg ctctggtcca cgatcgctcc
     4081 tacctcgacg ccctcgagcg cttctgcgcc agcggcggtg ggcacctcga ccccgacacg
     4141 gtggctgcgg cccgctcctg ggacatcgcc cgcctcgccg ccggcgcggg cctggacgcc
     4201 atcgaccggc tcaaggcggg cgaggccgac gccgcgttcc tggccgtccg cccgcccggg
     4261 caccacgcca ccccgtcgcg ggcgatgggc ttctgcctgc tgaacaacgt ggccgtggcg
     4321 gcggcggccc tcgccgatgc cggcgagcgc gtgctcgtcg tcgacatcga tgcccaccac
     4381 ggcaacggca cccaggacgc gttctgggac gacccccgcg tcacctacct ctcggtgcac
     4441 gagcacccga tgtaccccgg caccggcatg gtgcgcgaga ccggtggccc caacgctccg
     4501 gggaccacca tcaacctgcc ggtgccgtcg gccacctccg gcgacgtgta ccggcgcgtg
     4561 gtggacgacg tcatcgtccc ggtggccgag cgcatggccg tcacctggct gctgctgtcg
     4621 accgggttcg acgcccatcg caacgacccg ctcaccgacc tcggcctcag cagcggcgac
     4681 tactacgacc tcacgaagcg gatcctggcg ctggtgccgg ccggccggcg cctggccttc
     4741 ctcgagggcg gctacgacct cgacgccatc accacctcca cggcggcgac gctcgccgcc
     4801 ctggccggcg tcgagcaccg acccgaaccg gtgaccaccg acgccgccga cgcccccggt
     4861 caccaggcgg tcggggcggc gcgcctcatc caccagctcg cgtaacctcc cgccggggga
     4921 gggcccagat gaccgacacc accgagcacg tcgacgatca accggtggac cacgtcgcgc
     4981 tgctccgcgc cctgctcgag cggggcgggt ccgacctgca ctgcaagccg ggcagcccgc
     5041 cggcgctgcg ggtcggtggc gcgctgctgc gggagccgga cgatccggtc ctcggccagc
     5101 ggctcaccgc gccggcggtg gcgatgctgg cccagaccct gctcaccacc gaccagcagc
     5161 aggtcctcct gcaggacggg tcggtcgtcg ccacccacgc catccccgag gtcggccgct
     5221 tccgcgtcgc cgtctaccgc cagcgggggt cggtggccgt ggtcgtgcac gccgttcccc
     5281 agcaggtgct gcggctggag gagctgggcc tgccggattc ggtgtcgtcg ctcgcgtccg
     5341 ccgagcgcgg gctggtgctg gtggccagcc cggtcggcaa cggcgccacc accaccctcg
     5401 ccgccatggt cgaccacgtg aaccgcaccc gggcgtgcca cgtggtgacc gtggaggacc
     5461 ccgccgaggt cctccaccgc gacgaccgca gcctcatcag ccagctggag gtgcccaccg
     5521 acgtcgccgg cctggccgag ggcgtgcggg cggcatcccg cctcgacgcc gacgtgatca
     5581 ccatctccga catcgccgac cgcgacgtcg ccctggccgc gctcgacggc gtggcccggg
     5641 gccggttggt gctggccacc atcggcggca acacggtgcg ctcggtgatc gacggcttcc
     5701 tggagctgtt caccttcgag gagcgcccga acgtgcgcca gaccctggcc cggtccctca
     5761 ccggggtcgt ggtccagcgc ctgctcaccg gcaccgacgg acgcctcctg cccgtggccg
     5821 aggtgctggt ggccacggcc aagatccagg aggccctggc cgaggagtcc cgcatggggg
     5881 agctcggcgc gctcctggcc gacggggtgt tccacggcat gcagaccctc gaccagtccc
     5941 tccgcgacca ggtccgtggc gggcgcatca gcgaggccac cgccctgggc gcggccaccg
     6001 accccgagga gctgcggatc gagctgctgc gctcctgacg ggggtcggcg gagccgagcc
     6061 gacaggcgag gcgacccatg tcacgggccc tgtggcggcg gagccgccac gatcgaaccg
     6121 cggcgagacg taaccatctg cgc
//
LOCUS       DATISU010000060        12815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_282476, whole genome shotgun sequence.
ACCESSION   DATISU010000060 DATISU010000000
VERSION     DATISU010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 12815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12815
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282476"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..952
                     /locus_tag="VLR27_02675"
     CDS             74..952
                     /locus_tag="VLR27_02675"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSP02376.1"
                     /translation="MRPPATTLQLTTQQLEYLVAIDRHATWGEAAASVGVSPSALSQG
                     IAELERRLGVALFERDGRRRVPARDHEPVLRYAEATVAATRDLARWLASRRTGTTGVV
                     RLGMIDAAATHHFRDELRAHRRQRPEIDLLLAVDPSGPLLRRLVRGELDLVVCVETED
                     LPPGITTQPLLDDPLSVLAPGDVAEVGPAGWGPWVTFPSGSHTRQVVADALRRAGAGF
                     EVVAESNQPEVLREMALLGVGWTVLPLAGLGSVEGLRVVRRDLVVRRLVLARRAGARP
                     DPAVDELAATLLAGAQ"
     gene            963..2384
                     /locus_tag="VLR27_02680"
     CDS             963..2384
                     /locus_tag="VLR27_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HSP02377.1"
                     /translation="MQPDLDRSSALDLAAAIRSGEVSPTEVMDATLAAIADRNPALNS
                     VIWLDEDDARARAKAATDRLAADGPDDLPPFFGVPLPIKDLHPVQGWPCTFGSPGASD
                     APAEESSLVVQRLEDAGFLLCGMSNSPEFGTVTATENDRYGITRNPWDLDRTSGGSSG
                     GASAAVAGGMFTIAHASDGGGSIRIPATCTGLVGLKPARGRVVDTSVAWEGASTQGVV
                     SRTVADTAAALDAIAVFDPLAWWNAPAPERPFLDEIGADPGRLRIASLTTSPLGIDVD
                     PECAAAVQRTVDALADAGHEVVDHEFEPFAEDFIAGFVHVVNAGLSDFDVDWDRVQAN
                     NRANRGYAQKVDSLTYAGAVAALQRWTRKVNVQWGRDFDLLVTPTMPIQPPPAGQILR
                     EITEAPGDTSMTVLATVSYTSMFNMNGLPAISLPVHQATDTGLPVGAQLVAGPWQEAA
                     LLRVAAQVEQLLPWADRRPAPLP"
     gene            2297..3646
                     /locus_tag="VLR27_02685"
     CDS             2297..3646
                     /locus_tag="VLR27_02685"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP02378.1"
                     /translation="MAGGSAAARGRAGRAAASLGRPPSGPAALSRATPARLGAGFNRL
                     WTASVVSNTGDGVMAVALPLLAETLTRQPLLFAGVTVANRLPWLLFSLHAGAIADRVD
                     RRRLMATVNALRALLVAGLAVAVIADVASIWLLYVIGFGLGVAETLFDNASQAFLPAI
                     VERDDLERANGRLMAGEIVTNQFLGPPLGGFLFGLGAAVPILLDAGTFAISAGLIVSL
                     GGLVATRRPARDPDVVRPRMRTEIAEGLRWLRDHRLLRGLAVLLGAMNFTSTMLFAVF
                     PLYAVGEESALGLSGFGFGVLLTTTAIGSTIGSLLSARLVTRFGRGPMLWSTLVASVA
                     VPVVIAATSNAVVVAAAFAVFGLAVILWNVITVSLRQTIIPDRLLGRVNSVYRFLGWG
                     SMPLGSLAGGAAAALWGVRATFVISGLLMAVPIVIFAGVVRTSEIDAARAAAAADHP"
     gene            3711..5180
                     /locus_tag="VLR27_02690"
     CDS             3711..5180
                     /locus_tag="VLR27_02690"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HSP02379.1"
                     /translation="MPSTPDLSKKGAALAIGLAAAGWVVGRRLLSRGLGDGFSVRQGA
                     ARRRQLATMGGRAGGAFALHRARRTFASAERRQALDTEFEMRTAAQITETLGNMKGAM
                     MKLGQMASYLDSGMPPHVREALAQLQADAPPMSGELAAGVVESELGRHPDELFRQWDP
                     VPIASASIGQVHRAITRDGRAVAVKVQYPGIDEAIESDLGSANLVFGGLGVMFPGLES
                     RPIVEELRERILEELDYELEAANQQLFVDHYAGHPFIHVPAVVHELSSRRVLTTELAE
                     GARFDEVLTWSQEEKDLAAETIFRFVFRSLYRFRAFNGDPHPGNYLFRPGGQVVFLDF
                     GLVKRFTPEEVDTFSSLVRSMVLHRDVESFRRTIEEIGLLKPGEPFSDELVEEYFSHF
                     YQWVLEDGEATMTPAYASESIRRIFDASGPYGDIMKAANVPPSFVIIQRINLGLAALM
                     GELGATNNWRKIAEELWPFVDGPPATPMGEREHRWTTHR"
     gene            complement(5219..5491)
                     /locus_tag="VLR27_02695"
     CDS             complement(5219..5491)
                     /locus_tag="VLR27_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02380.1"
                     /translation="MDPATQRQQVLFLYLEHSGLDGRVVAWSFHDGSGRATDLDGAPP
                     YATGTAALRDGWRLLQVSPVPQRVPGAERVAGRLEHELVLERIVDA"
     gene            complement(5494..6375)
                     /locus_tag="VLR27_02700"
     CDS             complement(5494..6375)
                     /locus_tag="VLR27_02700"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HSP02381.1"
                     /translation="MTEEGEHLLATGDVAEFVARGFLALPAVVPDELNAAAVEELGQI
                     MATWGSDARPFAPSSGQRLSAIYPQPSAIGAVLRHPPVAGALRSLLGADPVFDHDFVH
                     HKPAGDPFGQPLHQDAMVDPTVAFDVQLFYFPNEVAPGGGGTGFVPGTHLRRVHETDV
                     GRYVHVAGERQWEGPAGSVVLFHQGLWHRGMPNPSGTDRLMYKIRLNPTEPQVRHWDT
                     GDLDELHNGPDDHLFARFRTDSVAGILRHREPWQGEADHRLELAARATLWRYLTGDER
                     FDVDWYLTRTEARAALA"
     gene            6421..6669
                     /locus_tag="VLR27_02705"
     CDS             6421..6669
                     /locus_tag="VLR27_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HSP02382.1"
                     /translation="MCRSITTLRGLEPAATPEEVEAAARQFVRKLSGIRHPSAATQEP
                     FDRAVADIAAAAVRMLEELPPRKQPPTTVPPNRRGHPI"
     gene            complement(6724..7992)
                     /locus_tag="VLR27_02710"
     CDS             complement(6724..7992)
                     /locus_tag="VLR27_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP02383.1"
                     /translation="MDFRLPDHLVSLLAELDTFIDDVIAPIQARDDNERFFDHRREDS
                     RTDWDRDGLPNEEWEALLGEMRRAADEAGFLRYHLPERFGGKGGSNFDMAVIREHLAA
                     KGLGLFNDLQNESSIVGNFPTVLMMEKYGSEAQQEEWIPKMLEGKAGIAFGLTEPNHG
                     SDATWMETRAERDGDEWVINGEKMWNTGVHKATHDFVFARTSGDDGEAAGISCFIVPM
                     SNDGVKIEEYYWTFNMPTDHARVSFTNVRVRAEDVLGPIDNGLQVAQLFVHENRIRQA
                     ASSLGAAQYCIDTAAKYAKDRRVFGKSLAANQAIQFPLAELHTEAAMLRALIRQTAWE
                     MDRVDHGMEISEKVAMCNYRGNRLVCDAADRAMQTCGGMGYSRHMAFEHIYRHHRRYR
                     ITEGAEEIQIRRVAAHLFGFTGAGRGSTAR"
     gene            8038..8787
                     /locus_tag="VLR27_02715"
     CDS             8038..8787
                     /locus_tag="VLR27_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02384.1"
                     /translation="MRRLVGVLGAIALLAACSSGGDDERALDGLGTPQLEPDTSAEGA
                     TSTQPAAVEGGPATSSPTSAPPGEDSAETTTTTVAGAAEATSVREIVQLDDPVGDATG
                     SLDGDPPRWADLAGASLERQGDAYRLVVRLGDVAPQSAPGAATMNVATFFDVDGDGGI
                     DHELWVNLGPDGWGPTWWTGDRARPGEASNVTVVVEGDELRLLFPDVMLDQPDRLRFS
                     LASEYGELGVIGSSYAHRDDAPDDDQAVTFP"
     gene            complement(8845..9153)
                     /locus_tag="VLR27_02720"
     CDS             complement(8845..9153)
                     /locus_tag="VLR27_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02385.1"
                     /translation="MKTKLGASLIAALMMMGLFGTVSATADPGPNDKNNKGLCTAYFN
                     GSDTGRANKRNAPPFQGLEAAAEAADETVAEFCEGLVGGNPNYEDPEGANPNSRGGRS
                     "
     gene            9273..9761
                     /locus_tag="VLR27_02725"
     CDS             9273..9761
                     /locus_tag="VLR27_02725"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HSP02386.1"
                     /translation="MRSLSLALRIGTSIAAVAALTLAAATPAGAHAELLESDPPVGGT
                     VTEGVETVQMTLLAFDPNGPVDIEVTDPAGTDVTEGEPRVDARSSTVEIDVEPLGVGQ
                     HIVHWHAMADDGDGESEGTFTFEVKKAQGGGWGIWLVWLFALGVPAAIFLRPGGRRGS
                     ER"
     gene            9758..10462
                     /locus_tag="VLR27_02730"
     CDS             9758..10462
                     /locus_tag="VLR27_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSP02387.1"
                     /translation="MTAPLAGVTHLGIDADDTLWHSENRFHETHQRYHALLADHVDLD
                     VDELERRMLETEQRNLRLFGYGAKGFTLSLIETAIEVTDGAVPTEVISTILEFGKDLL
                     DHPVELLDGVADAIEAFRARGLHLLLITKGDLWHQESKVAASGIADHFEAVEIVAEKD
                     AGTYRAILDRHSIEPASFCMIGNSVRSDVLPVLELGGRAVHVPYAYLWAHEAVDDPGD
                     EHLVARALREVAELFG"
     gene            10518..11954
                     /locus_tag="VLR27_02735"
     CDS             10518..11954
                     /locus_tag="VLR27_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HSP02388.1"
                     /translation="MAVQRLLDPTPVRSVEEHRAVGGLIGLDDARRVEPEVAISVVTD
                     SGLRGRGGAGFPTGKKWSTVAANAASGDLAPTVVVNAAEGEPGTFGDRTLIRRNPYRV
                     LEGALIAAHAIGARAIAVVTKEQYTTELELLTDAAQDLVATGAAGDVEIDVVGGAGHY
                     LLGEETGLLEAAAGRLPFPRVAPPYRHGTVEVGDPSGGASRLELAGDGGTAPPTLVNN
                     AETFAHVPQIVQFGSDWFRELGTADSPGTSLFTISGDTRRGGVAEFPLGTPVREIIEE
                     LGGGMTDAEIAFVMSGVGSGILLPHQLDLPATYEDLSAGGSGLGTGGFMVFSTDTDPI
                     AVAHGVSRFLAIESCGQCSPCKEGGLEVAAALDVLRDGQAGDTAVEGLLPLVRTLSDE
                     ARCDLGPQHERVITSFLEQFPDALRAHAVDHVSAAPVLVAPIVDIDDEGRVTLDEDHR
                     TKQPDWDHGDDWNGQTPADRHDVAVTIE"
     gene            11996..>12815
                     /locus_tag="VLR27_02740"
     CDS             11996..>12815
                     /locus_tag="VLR27_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP02389.1"
                     /translation="MSQPVAIRLEQVCKRYPGATSYAVDHLDLEIPTGEIVALVGPSG
                     CGKTTTLKMINRLIEPTSGTIEIHGRPAGELPVHELRRGIGYVIQQTGLFPHKTVAGN
                     IATVPRLLGWDKARTRERVEELVDLVGLERQMLDRYQSALSGGQQQRVGVARALAADP
                     PILLMDEPYRAVDPIVRARLQDELLDLQRRVHKTIVLVTHDVDEALKLGDRVVILNVG
                     GVLEQAARPAEILADPASPFVADFLGEERGLKRLALLRVRDITPDTGPVAEVGDD"
BASE COUNT         1755 a         4898 c         4320 g         1842 t
ORIGIN      
        1 tcatgccccc atcatgaagc atcatccagc gaacgcaaca atggttcagg aacactgaac
       61 taacgtcgcg ggggtgcgac ccccggcgac cacgctgcag ctcaccaccc agcagctcga
      121 gtacctcgtg gccatcgacc gccacgccac gtggggcgag gctgccgcct ccgtcggcgt
      181 atcaccgtcg gcgctgtccc agggcatcgc cgagctcgag cgccgactcg gcgtcgccct
      241 gttcgagcgc gacgggcgtc ggcgggtacc ggcccgggac cacgagcccg tcctgcgcta
      301 cgccgaggcc accgtcgccg cgacgcgcga cctggcccgg tggctggcgt cgcgccgcac
      361 cggcaccacc ggcgtggtcc gcctgggcat gatcgacgcc gccgcgaccc accacttccg
      421 cgacgagctg cgcgcccacc ggcgccagcg ccccgagatc gacctcctgc tggccgtcga
      481 cccgtccggg ccgctgctgc ggcgactggt gcgcggcgag ctcgacctcg tggtgtgcgt
      541 cgagaccgag gacctccccc cgggcatcac cacccagccg ctgctggacg accccctgag
      601 cgtgctggca ccaggcgacg tcgccgaggt cgggccggcc ggctggggcc cctgggtcac
      661 gttcccgagc ggctcgcaca cccgtcaggt cgtcgccgac gccctgcgcc gcgccggggc
      721 cggcttcgag gtcgtcgccg agtccaacca gcccgaggtc ctgcgcgaga tggccctgct
      781 cggcgtgggc tggacggtgc tgccgctggc cggcctcggc tcggtcgagg ggctccgggt
      841 ggtgcgccgc gacctcgtgg tccgccgcct cgtcctggcc cgccgagcgg gggcccgacc
      901 cgacccggcc gtggacgagc tcgccgccac cctgctggcg ggcgctcagt agcgtgcgcg
      961 ccgtgcagcc cgacctcgac cgcagctccg ccctcgacct ggccgcggcc atccgcagcg
     1021 gcgaggtctc ccccaccgag gtcatggacg ccaccctggc cgccatcgcc gaccgcaacc
     1081 cggccctcaa ctccgtgatc tggctcgacg aggacgacgc ccgggcgcgg gcgaaggccg
     1141 ccaccgaccg cctcgccgcc gacggacccg acgacctccc gccgttcttc ggcgtacccc
     1201 tgccgatcaa ggacctgcac ccggtgcagg gctggccctg cacgttcggc tcgccgggcg
     1261 cctcggatgc gccggcggag gagagcagcc tcgtcgtgca gcgcctcgag gacgccggct
     1321 tcctgctctg cggcatgagc aactccccgg agttcggcac cgtcaccgcc accgagaacg
     1381 accgctacgg catcacccgc aacccgtggg acctcgaccg cacctcgggc ggctcctccg
     1441 gcggggcgtc cgcagccgtg gccggcggga tgttcaccat cgcccacgcc agcgacggcg
     1501 gcggctccat ccgcatcccc gccacctgca ccggcctcgt cgggctgaag ccggcccgag
     1561 gtcgtgtcgt ggacacctcc gtcgcctggg agggcgcctc cacccagggc gtggtgtcgc
     1621 gcaccgtggc cgacaccgcg gcggccctcg acgccatcgc cgtcttcgat ccgctcgcct
     1681 ggtggaacgc cccggcacca gagcgcccgt tcctcgacga gatcggcgcc gaccccggcc
     1741 gcctccgcat cgcgtcgctc accacgtccc cgctcggcat cgacgtcgac ccggagtgcg
     1801 cagccgccgt gcagcgcacc gtcgacgcac tggccgacgc cggccacgag gtcgtcgacc
     1861 acgagttcga acccttcgcc gaggacttca tcgccggctt cgtgcacgtc gtcaacgccg
     1921 ggctgtccga cttcgacgtg gactgggacc gggtccaggc caacaaccgg gccaaccggg
     1981 gctacgccca gaaggtcgac agcctcacct acgccggcgc cgtcgccgcg ctgcagcgct
     2041 ggacccgcaa ggtgaacgtc cagtggggcc gggacttcga cctcctcgtc acgccgacca
     2101 tgccgatcca gcccccgccg gccgggcaga tcctgcggga gatcaccgag gcgcccggcg
     2161 acacctcgat gaccgtgctc gccacggtga gctacacgtc gatgttcaac atgaacggtc
     2221 tgcccgccat ctccctgccg gtgcaccagg ccaccgacac cggcctgccc gtgggcgccc
     2281 agctcgtcgc cggtccgtgg caggaggcag cgctgctgcg cgtggccgcg caggtcgagc
     2341 agctgcttcc ctgggccgac cgccgtccgg ccccgctgcc ctgagccgcg ccacgccggc
     2401 ccgcctcggc gccggcttca accgcctgtg gacggcgtca gtcgtctcca acaccggcga
     2461 cggggtcatg gccgtggccc tgccgctgct ggccgagacc ctgacccgcc agcccctgct
     2521 gttcgccggc gtcaccgtcg ccaaccggct cccgtggctg ctcttctcgc tgcacgccgg
     2581 cgccatcgcc gaccgggtcg accggcgtcg gctcatggcc acggtgaacg cgctgcgggc
     2641 gctgctggtc gccgggctcg ccgtcgccgt gatcgccgac gtggccagca tctggctgct
     2701 ctacgtcatc ggcttcggcc tcggggtcgc cgagacgctg ttcgacaacg cctcccaggc
     2761 cttcctcccc gccatcgtcg agcgcgacga cctcgagcgg gcgaacggtc gcctcatggc
     2821 cggcgagatc gtcaccaacc agttcctcgg gccgcccctc ggtggcttcc tgttcggcct
     2881 cggcgccgcc gtgccgatcc tgctcgacgc cggcacgttc gccatctccg ccgggctcat
     2941 cgtgtccctg ggcggcctcg tcgcgacccg ccggccagcg cgcgatcccg atgtcgtgcg
     3001 tccccgcatg cgcaccgaga tcgccgaagg gctccgctgg ctgcgagacc accgcctgct
     3061 ccggggcctc gccgtgctgc tcggcgccat gaacttcacc tccacgatgc tgttcgccgt
     3121 cttcccgctg tacgccgtgg gcgaggagtc cgccctcggg ctcagcgggt tcgggttcgg
     3181 cgtgctgctc acgaccacgg ccatcgggtc caccatcggg tcgctgctct cggcccggct
     3241 cgtcacccgc ttcggtcgcg ggccgatgct gtggagcacc ctcgtcgcgt ccgtcgccgt
     3301 gccggtcgtg atcgccgcca cctcgaacgc ggtcgtcgtc gctgcggcgt tcgccgtgtt
     3361 cgggctcgcc gtcatcctct ggaacgtgat caccgtctcg ctccgccaga cgatcatccc
     3421 cgaccggctc ctcggccggg tcaacagcgt ctaccggttc ctcggctggg gctccatgcc
     3481 cctcggcagc ctcgccggcg gcgccgcggc cgccctgtgg ggcgtgcgcg ccacgttcgt
     3541 gatctccggg ctgctcatgg ccgtgccgat cgtgatcttc gccggcgtcg tccgcaccag
     3601 cgagatcgac gccgcccgcg ctgcggcggc cgcggaccac ccctgaggaa ctggcagttc
     3661 tcctgtcgcg ccttttccac cggcatggcc gcgtccgtac gatccgcggc gtgccgagca
     3721 cccccgatct gtccaagaag ggcgcggccc tcgccatcgg cctggcggcg gccgggtggg
     3781 tcgtcggacg gcgcctgctc agccggggcc tgggcgacgg cttctcggtc cgccagggcg
     3841 cggcacgtcg acgccagctg gcgaccatgg gtgggcgcgc cggcggcgcg ttcgcgctcc
     3901 accgggcccg ccgcaccttc gccagcgccg agcgccgcca ggccctcgac accgagttcg
     3961 agatgcgcac cgccgcgcag atcaccgaga cgctcgggaa catgaagggc gccatgatga
     4021 agctcggcca gatggccagc tacctcgact ccggcatgcc gccccacgtc cgcgaggcgc
     4081 tggcccagct gcaggccgac gcacctccga tgagcggcga gctggccgcc ggcgtcgtcg
     4141 agtccgagct cggccggcac cccgacgagc tgttccgcca gtgggacccc gtgccaatcg
     4201 cgtcggcctc catcggccag gtccaccggg cgatcaccag ggacggtcgg gcggtcgcgg
     4261 tgaaggtcca gtacccgggc atcgacgagg ccatcgagtc cgacctcggc agcgccaacc
     4321 tggtcttcgg cggcctcggc gtgatgttcc ccgggctgga gtcccgcccg atcgtggagg
     4381 agctccgcga gcggatcctc gaggagctcg actacgagct ggaggcggcc aaccagcagc
     4441 tgttcgtcga ccactacgcc gggcacccct tcatccacgt gccggcggtg gtgcacgagc
     4501 tcagcagccg ccgcgtgctc accaccgagc tggccgaggg cgcccgcttc gacgaggtcc
     4561 tcacctggtc ccaggaggag aaggacctgg cggccgagac catcttccgc ttcgtgttcc
     4621 gcagcctcta ccggttccgg gccttcaacg gcgatcccca cccgggcaac tacctgttcc
     4681 ggccgggcgg ccaggtggtc ttcctcgact tcggcctggt gaagcgcttc acgccagagg
     4741 aggtcgacac cttctcctcg ctcgtgcgct cgatggtgct gcaccgcgac gtcgagtcct
     4801 tccggcgcac catcgaggag atcggcctgc tgaagccggg cgagccgttc tccgacgagc
     4861 tggtcgagga gtacttcagc cacttctacc agtgggtcct ggaggacggt gaggccacca
     4921 tgacgccggc gtacgcgtcc gagtcgatcc gccggatctt cgacgccagc ggcccctacg
     4981 gcgacatcat gaaggcggcc aacgtcccgc cgtcgttcgt catcatccag cgcatcaacc
     5041 tcgggctggc cgcgctcatg ggcgagctgg gggcgacgaa caactggcgc aagatcgccg
     5101 aggagctgtg gcccttcgtc gacggaccgc ccgccacgcc gatgggcgag cgcgagcacc
     5161 gctggaccac ccaccgctga tcgcccgcgg gatcgttctg gcagcggcca cggaacgctc
     5221 aggcgtcgac gatgcgctcc aggaccagct cgtgctcgag gcggccggcc acgcgctcgg
     5281 cgcccgggac gcgctggggc accggcgaca cctggagcag ccgccagccg tcgcgcagcg
     5341 ccgcggtacc ggtggcgtac ggcggcgccc cgtcgaggtc ggtggcgcgg ccgctgccgt
     5401 cgtggaacga ccacgccacg acccgaccgt ccaggccgct gtgctcgagg tagaggaaca
     5461 gcacctgctg gcgctgggtc gccgggtcca tcgtcatgcc aacgcggccc gcgcttcggt
     5521 gcgggtcagg taccagtcga cgtcgaagcg ttcgtcgccc gtgaggtagc gccacagcgt
     5581 cgcccgggcg gccagctcca gccggtggtc ggcctcgccc tgccacggtt cgcggtggcg
     5641 caggatgccg gcgaccgagt cggtgcggaa ccgggcgaag aggtggtcgt cgggaccgtt
     5701 gtggagctcg tccaggtctc cggtgtccca gtggcgcacc tgcggctcgg tcgggttgag
     5761 ccggatcttg tacatcagcc gatcggtccc cgacgggttc ggcatccccc ggtgccacag
     5821 accctggtgg aacagcacga cgctgccggc cggtccctcc cactgccgct cgccggcgac
     5881 gtgcacgtag cggccgacgt cggtctcgtg gacccggcga aggtgggtgc cgggcacgaa
     5941 cccggtgccg ccgccaccag gcgccacctc gttcgggaag tagaagagct ggacgtcgaa
     6001 cgcgacggtg gggtcgacca tggcgtcctg gtggaggggt tgcccgaacg ggtccccggc
     6061 cggcttgtgg tgcacgaagt cgtggtcgaa caccggatcg gcgccgagca gggaccggag
     6121 cgcaccggcg accggcgggt ggcgcagcac ggcaccgatc gccgacggct gcggatagat
     6181 cgccgagagc cgctggccac tcgagggagc gaacggtcgg gcatcggatc cccaggtggc
     6241 catgatctgc ccgagctcct cgacggcggc ggcgttgagc tcgtcaggga ccactgccgg
     6301 cagggcgagg aacccgcggg cgacgaactc ggccacgtcg cccgtggcca gcaggtgctc
     6361 cccctcttcg gtcatggatc gggacactac gtgctggtca cgatcgatac cgtcgagcac
     6421 atgtgccgat cgatcaccac ccttcgcggg ctcgaaccgg cggccacgcc ggaggaggtc
     6481 gaggccgccg cccggcagtt cgtccgcaag ctcagcggca tccgccaccc gagcgcggcg
     6541 acccaggagc ccttcgaccg ggccgtcgcc gacatcgccg ccgccgcggt ccggatgctc
     6601 gaggagctcc cgccccgcaa gcagcccccg acgacggtcc cgcccaaccg ccgaggccac
     6661 cccatctagg aaattccgtg gatcgagacc atcggcggta tccgatccac gggatttcgg
     6721 ggctcagcgg gcggtggagc cccggccggc gcccgtgaag ccgaacaggt gggcggcgac
     6781 ccggcggatc tggatctcct cggcgccctc ggtgatccgg tagcgacggt ggtggcggta
     6841 gatgtgctcg aaggccatgt gccgcgagta ccccatgccg ccgcaggtct gcatggcgcg
     6901 gtcggcggca tcgcacacga ggcggttgcc gcggtagttg cacatggcga ccttctcgga
     6961 gatctccatg ccgtggtcga cccggtccat ctcccacgcg gtctgccgga tcagggcccg
     7021 cagcatggcg gcctcggtgt gcagctcggc cagggggaac tggatggcct ggttcgcggc
     7081 cagcgacttg ccgaacaccc ggcgatcctt ggcgtacttg gccgcggtgt cgatgcagta
     7141 ctgcgcggcg ccgagcgagg aggcggcctg gcggatccgg ttctcgtgca cgaagagctg
     7201 ggcgacctgc aggccgttgt cgatcgggcc gagcacgtcc tcggcccgca cccgcacgtt
     7261 ggtgaacgac acccgggcgt ggtcggtcgg catgttgaag gtccagtagt actcctcgat
     7321 cttcaccccg tcgttggaca tcggcacgat gaagcacgag atgccggcgg cctcgccgtc
     7381 gtcgccgctg gtgcgggcga acacgaagtc gtgggtggcc ttgtggaccc cggtgttcca
     7441 catcttctcg ccgttgatga cccattcgtc accgtcgcgc tcggcgcggg tctccatcca
     7501 ggtggcatcg gacccgtggt tgggctcggt gaggccgaag gcgatgccgg ccttgccctc
     7561 cagcatcttg gggatccact cctcctgctg ggcctccgag ccgtacttct ccatcatcag
     7621 caccgtcggg aagttgccga cgatggagct ctcgttctgc aggtcgttga acagcccgag
     7681 ccccttggcc gccaggtgct cccggatcac cgccatgtcg aagttcgacc cgcccttgcc
     7741 accgaagcgc tcgggcaggt ggtagcgcag gaagccggcc tcgtcggcgg cgcgccgcat
     7801 ctccccgagc agcgcctccc actcctcgtt cgggagacca tctcgatccc aatccgtgcg
     7861 ggaatcctcg cgtcggtggt cgaagaaccg ctcgttgtcg tcacgcgcct ggatgggcgc
     7921 gatgacgtcg tcgatgaagg tgtcgagctc ggcgagcagg gagacgagat gatcgggcag
     7981 ccggaagtcc atggggcgaa ggtaacgcac gttgccaacc gtcgtcgtcc cccggcggtg
     8041 cgacgcctgg tcggcgtgct gggcgccatc gcgctcctgg ccgcatgctc gtccggcggc
     8101 gatgacgagc gagcgctcga cggcctgggc acgccgcagc tcgagccgga cacctcggcg
     8161 gagggcgcca ccagcacgca acccgcggcc gtcgaaggcg gacccgccac gagcagcccg
     8221 acgagcgcac ctcccggcga ggactcagca gagacgacca ccacgaccgt ggccggagcg
     8281 gccgaggcga ccagcgtccg cgagatcgtc cagctcgacg accccgtcgg cgatgccacc
     8341 ggcagcctcg acggggaccc gccccgctgg gccgacctgg ccggcgcctc gctggagcgc
     8401 cagggcgacg cctaccggct cgtcgtccgc ctcggcgatg tcgcaccgca gtcggccccg
     8461 ggtgccgcga ccatgaacgt ggccacgttc ttcgatgtcg acggcgacgg cggcatcgac
     8521 cacgagctgt gggtgaacct cgggcccgac ggctgggggc cgacgtggtg gaccggcgac
     8581 cgcgcccgcc cgggcgaggc atcgaacgtc accgtcgtgg tggagggcga cgagttgcgc
     8641 ctgctcttcc ccgacgtcat gctcgaccag cccgaccgcc tccgcttctc actggcgtcg
     8701 gagtacggcg agctcggtgt catcggctcc agctacgccc accgggacga cgctcccgac
     8761 gacgaccagg ccgtcacctt cccgtagacg cggaacgggg acccgccgca gcgggtcccc
     8821 gttcgatgcc gttggctcag tccgtcagga ccggccgccc cggctgttgg ggttcgcacc
     8881 ctcggggtcc tcgtagttcg ggttcccccc gaccaacccc tcacagaatt ccgcaaccgt
     8941 ctcatccgct gcttcagcgg cggcttcgag tccctggaac ggcggtgcgt tccgcttgtt
     9001 cgcccgaccg gtgtcagacc cgttgaagta cgcggtgcag aggcccttgt tgttcttgtc
     9061 gttcggtccc ggatcggcgg tcgcgctgac cgtcccgaac agacccatca tcatcagcgc
     9121 cgcgatcagc gacgcaccca gcttcgtctt catctgctcc ccttgattcg ttccgagttg
     9181 cccacccggc ccggtcctcc tttcggcaga atcgctgccc gttcctgccg gtaacccgca
     9241 acttcctgcc tgcgggcgcc agactcgatc ccatgcgatc gctgtccctc gcgctccgga
     9301 tcggcacgtc gatcgctgcc gtcgcggccc tgacgctcgc cgccgccacc ccggccggcg
     9361 cccacgccga gctgctcgag tccgaccctc cggtcggcgg gaccgtgacg gagggcgtgg
     9421 agacggtcca gatgaccctg ctcgccttcg atccgaacgg tccggtggac atcgaggtca
     9481 ccgacccggc cggcaccgac gtcaccgagg gcgaaccgcg cgtcgacgcc cgctcgtcga
     9541 cggtggagat cgacgtggaa ccgctcggcg tcggccagca catcgtccac tggcacgcga
     9601 tggccgacga cggggacggt gagagcgaag gcacgttcac cttcgaggtc aagaaggccc
     9661 agggcggcgg ctggggcatc tggctcgtgt ggctgttcgc cctcggcgtg cccgcggcga
     9721 tcttcctccg accgggcggc cgccgcggga gcgaacggtg acggcaccgc tggccggcgt
     9781 gacccacctc ggcatcgacg ccgacgacac gctctggcac tcggagaacc gcttccacga
     9841 gacgcaccag cgctaccacg cgctgctcgc cgaccacgtc gatctcgacg tcgatgagct
     9901 cgaacgacgg atgctggaga ccgagcagcg caacctgcgc ctgttcggct acggcgccaa
     9961 ggggttcacc ctctccctca tcgagaccgc catcgaggtc accgacggcg ccgtcccgac
    10021 cgaggtcatc tccaccatcc tcgagttcgg caaggacctc ctcgaccacc cggtcgagct
    10081 gctcgacggg gtcgccgacg ccatcgaggc cttccgtgcc cgcggcctgc acctgctgct
    10141 gatcaccaag ggcgacctct ggcaccagga gtccaaggtc gccgcgtccg gcatcgccga
    10201 ccacttcgag gcggtggaga tcgtcgccga gaaggacgcc ggcacgtacc gggccatcct
    10261 cgatcgccac agcatcgagc cggcgagctt ctgcatgatc ggcaacagcg tgcgctccga
    10321 cgtcctgccg gtgctggagc tgggcggccg cgccgtccat gtgccctacg cctacctgtg
    10381 ggcccacgag gcggtggacg accccggcga cgagcacctc gtggcccgag ccctccgaga
    10441 ggtcgccgag ctgttcggct gaccgcaggt cccgtcaccg ggcatgccac gacctggccg
    10501 ggggtacgga cgcggccatg gctgtccaga gactgctcga tccgacgccc gtccgctccg
    10561 tcgaggagca ccgagccgtc ggggggctca tcggcctcga cgacgcccgg cgcgtggagc
    10621 ccgaggtggc catcagcgtc gtcaccgact ccggcctccg tggccgcggt ggcgccggct
    10681 tccccaccgg caagaagtgg tccaccgtcg cggcgaacgc ggcgagcggc gacctcgctc
    10741 ccaccgtggt cgtcaacgct gccgagggcg agcccggcac gttcggcgac cgcaccctca
    10801 tccgccgcaa cccgtaccgg gtgctcgaag gcgccctcat cgccgcccac gccatcggcg
    10861 cccgggccat cgccgtggtc accaaggagc agtacaccac cgagctcgag ctgctcaccg
    10921 acgccgccca ggacctggtg gccaccggcg cagccggcga cgtggagatc gacgtcgtgg
    10981 gcggcgccgg ccactacctc ctcggtgagg agaccgggct gctcgaggcc gccgccggtc
    11041 gcctcccgtt cccccgcgtg gcacccccct accggcacgg cacggtcgag gtcggcgacc
    11101 cctccggcgg cgccagccgg ctggagctgg ccggcgacgg cggcaccgct ccgccaaccc
    11161 tcgtcaacaa cgccgagacc ttcgcccacg tgccccagat cgtgcagttc ggctccgact
    11221 ggttccgcga gctcggcacg gccgactccc cgggcacgtc gctgttcacc atctcgggcg
    11281 acacccgacg gggcggcgtc gccgagttcc cgctcggcac cccggtccgc gagatcatcg
    11341 aggagctcgg cggcggcatg accgacgccg agatcgcctt cgtgatgagc ggcgtcggct
    11401 ccggcatcct gctcccccac cagctcgacc tgcccgccac ctacgaggac ctgtccgccg
    11461 gtggctccgg cctcggcacc ggcgggttca tggtcttcag caccgacacc gatcccatcg
    11521 ccgtcgccca cggcgtgtcc cgcttcctgg ccatcgagtc gtgcggccag tgcagcccct
    11581 gcaaggaggg cgggctggag gtcgccgccg ccctcgacgt gctccgcgac ggccaggccg
    11641 gcgacaccgc ggtcgagggt ctgctgccgc tggtccgcac gctctcggac gaggcccggt
    11701 gcgatctcgg tccgcagcac gagcgggtga tcacgagctt cctggagcag ttccccgacg
    11761 ccctccgggc ccatgcggtc gaccacgtca gcgctgctcc cgtgctggtc gctcccatcg
    11821 tggacatcga cgacgagggg cgggtcacgc tcgacgagga ccaccgcacc aagcagccgg
    11881 actgggacca cggtgacgac tggaacggcc agaccccggc cgaccgccac gacgtcgccg
    11941 tcaccatcga gtagcgacgg cacccggcgc cagcgacgtc gctaggttgc cccggatgtc
    12001 gcagcctgtc gcgatccgac tcgagcaggt ctgcaagcgc taccccggtg cgacctcgta
    12061 cgcggtcgac cacctcgacc tcgagatccc caccggcgag atcgtggccc tcgtcggtcc
    12121 gtccggctgc ggcaagacca ccaccctcaa gatgatcaac cggctcatcg agccgacgtc
    12181 gggcaccatc gagatccacg gccgcccggc cggcgagctc ccggtgcacg agctgcgccg
    12241 cggcatcggc tacgtgatcc agcagaccgg cctgttcccc cacaagacgg tggccggcaa
    12301 catcgccacc gtgccccgcc tgctggggtg ggacaaggcc cgcacccggg agcgggtcga
    12361 ggagctggtc gacctcgtgg gactcgaacg ccagatgctc gaccgctacc agtcggcgct
    12421 gtccggcggt cagcagcagc gcgtcggcgt cgcccgggcc ctggccgcgg atccgccgat
    12481 cctgcttatg gacgagccct accgcgccgt cgacccgatc gtccgggccc gcctgcagga
    12541 cgagctgctc gacctgcagc gccgcgtgca caagacgatc gtgctcgtga cccacgacgt
    12601 cgacgaggcc ctgaagctcg gcgaccgggt cgtcatcctc aacgtcggcg gcgtgctcga
    12661 gcaggcggcg cgaccggcgg agatcctggc cgacccggca tcgcccttcg tcgccgactt
    12721 cctcggcgag gagcgggggc tgaagcgcct cgcgctgctg cgcgtccgcg acatcacgcc
    12781 cgacaccggc cccgtcgccg aggtcggcga cgacg
//
LOCUS       DATISU010000061         3695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_468670, whole genome shotgun sequence.
ACCESSION   DATISU010000061 DATISU010000000
VERSION     DATISU010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468670"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..568)
                     /gene="ndhC"
                     /locus_tag="VLR27_02745"
     CDS             complement(95..568)
                     /gene="ndhC"
                     /locus_tag="VLR27_02745"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSP02390.1"
                     /translation="MLGQYLPLVVLMVLAAVFVAGSLVTSRLVAPQRKTVAKLAPYES
                     GIVPTREPPERFPVKFFLVAMSFVVFDIEIIFLFPWATIYRQLGAFGLFAILLFSAPI
                     VISLAYEFSRGALDWGPAGRREPLDAGIVSRERTAASTVRRVGLEGRPTSTSEVA"
     gene            complement(571..1509)
                     /locus_tag="VLR27_02750"
     CDS             complement(571..1509)
                     /locus_tag="VLR27_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02391.1"
                     /translation="MQTLNLIAGIAFDPTIRGILAVLVGVVVLMGSVYLLLATNTGNR
                     LGFLLALTGFFGWMAIMGSIWWIYGIGMQGQAPEWDVVEYNAGDVDQAVTDKVTELEL
                     SDLPQDDDGVDYEAMSTLAEEDPERFVEINEQFEEDSNGWRWMPASDPSRGEAEATVS
                     DALPECSTCNFGIENPADFLVLGAFEVGGKEGLPADPSRWDRIRTQIVSAFTIKNPER
                     YAVVQVQRVIEQEPVPGEAPPTPEVNPDEPVISVVMIRDIGDRRFPAAMITIGSTLIF
                     ALLCWVLHQRERILEANLAHAEAVRNGTAVPTGATV"
     gene            complement(1509..2642)
                     /locus_tag="VLR27_02755"
     CDS             complement(1509..2642)
                     /locus_tag="VLR27_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSP02392.1"
                     /translation="MTDLTTAIFAASTVEAIGTVIALLAVVAVLVYAAFNVRAGRKEV
                     GSELELAANLKPYLDDDELETKKLDRALTAGLAGLVLVGVGLPAYWLGEPGRHEGAEE
                     QFASVFESRGEELYTEGAQCNACHGPEGVGGVASHTILDADNEFVAQVNWEAPALDTV
                     LLRYDRDEVYYILEYGRPYSPMPAWGEGGGGPLTEQQLENLIDYMASIQISSEESKDA
                     VESQLRTQLELGEDEDVDYTDLATGEALFNLGQDNGFAGGAYSCGRCHTRGWSINVES
                     AAPDTADLDPYVDYEDGSGGYGPSLRGGIVPRQFANVEELAEFLHVGAERGVPYGLNG
                     LSGDGMMPGFGDNPNTEEAEDGMMTTEMIEAIARYVESLGEDD"
     gene            complement(2703..3578)
                     /locus_tag="VLR27_02760"
     CDS             complement(2703..3578)
                     /locus_tag="VLR27_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSP02393.1"
                     /translation="MDTSTIAIIAVVALVVLAGVVLLAAARRNQTQKAIGVLSRETRS
                     RDAGAATITPREEGKATASGRELELAAKLERSRSGKELVTAGSSAPTAWVPPDPDTLG
                     ATRRQFLNRGITGFFIVGLSGFGAACIAFLWPQLGGGFGSVISVGNVSDVISQIRDNN
                     GFLYQPEGRMWLTEYPTSALEKARAVYSPGELAGMEEGVIALYQKCPHLGCRVPECVT
                     SQWFECGCHGSMYNRVGEKKGGPAPRGLDRFAMSVSGGVLSVDTGTIIQGPAIGTNTT
                     GQEAEGPNCIGAASH"
BASE COUNT          553 a         1187 c         1392 g          563 t
ORIGIN      
        1 tcctccaccc gtccggtgag cacgttgtga tcgagccctt cgaggccctt gtcgagctgc
       61 caccccatgg gggactcctc tctcggctct tcggtcaggc gacttcggac gtgctggtgg
      121 gtcggccctc gaggccgacc cggcgcacgg tggatgcggc ggtgcgctcc cgggacacga
      181 tgccagcgtc gagtggctcg cgccgaccgg cgggacccca gtcgagggcg ccgcggctga
      241 actcgtacgc cagcgagatc acgatcggtg ccgagaacag caggatcgcg aacaggccga
      301 aggccccgag ctggcggtag atcgtggccc acgggaacaa gaagatgatc tcgatgtcga
      361 agaccacgaa cgacatggcc accaggaaga acttcaccgg gaagcgctcc ggtggctccc
      421 gggtggggac gatgccggac tcgtagggcg ccagcttcgc caccgtcttg cgctgggggg
      481 cgaccaaccg cgacgtcacc aggctgccgg ccacgaacac cgcggcgagc accatgagga
      541 cgacgagcgg gaggtactgg cccagcatgg tcagacggtg gctccggtgg gcacggcggt
      601 gccgttgcgg accgcctcgg cgtgggcgag gttggcctcg aggattcgct cacgctggtg
      661 cagcacccag cacagcaggg cgaagatgag cgtggagccg atggtgatca tcgccgccgg
      721 gaagcgccgg tcgccgatgt cgcggatcat gaccacggag atgaccggct cgtcggggtt
      781 gacctcgggc gtgggcgggg cctcgccggg cacgggctcc tgctcgatga cccgctggac
      841 ctggaccacg gcgtagcgct cggggttctt gatggtgaac gccgacacga tctgggtgcg
      901 gatgcggtcc cagcggctgg ggtcggccgg caggccctcc ttgcccccga cctcgaaggc
      961 accgaggacg aggaagtcgg ccgggttctc gatgccgaag ttgcaggtgg agcactcggg
     1021 cagcgcgtcg ctcacggtgg cctcggcctc gccgcgggag gggtcggacg cgggcatcca
     1081 gcgccagccg ttgctgtcct cctcgaactg ctcgttgatc tcgacgaagc gctcggggtc
     1141 ctcctcggcc agcgtggaca tggcctcgta gtcgacgccg tcgtcgtcct ggggcaggtc
     1201 cgacagctcg agctcggtga ccttgtcggt cacggcctgg tcgacgtcgc cggcgttgta
     1261 ctcgaccacg tcccactcgg gggcctggcc ctgcatgccg atgccgtaga tccaccagat
     1321 ggagcccatg atcgccatcc agccgaagaa gccggtgagg gcgagcagga agccgaggcg
     1381 gttgccggtg ttggtggcga gcagcaggta caccgagccc atcagcacga cgacgccgac
     1441 gagcaccgcg aggatgccgc ggatggtggg gtcgaacgcg atgcccgcga tcaggttgag
     1501 ggtctgcatc agtcgtcctc cccgagggac tcgacgtagc gggcgatggc ctcgatcatc
     1561 tccgtggtca tcatcccgtc ctcggcctcc tcggtgttcg ggttgtcacc gaagcccggc
     1621 atcatgccgt cgccggacag gccgttgagg ccgtagggca cgccgcgctc ggcgccgacg
     1681 tgcaggaact cggcgagctc ctcgacgttg gcgaactggc gagggacgat gccgccccgc
     1741 agcgaggggc cgtagccgcc cgagccgtcc tcgtagtcga cgtaggggtc gaggtcggcg
     1801 gtgtcgggag cggcgctttc gacgttgatg gaccagccgc gagtgtggca gcgaccgcag
     1861 gagtaggcgc cgcccgcgaa gccgttgtcc tggccgaggt tgaacagcgc ctcgccggtg
     1921 gcgaggtcgg tgtagtcgac gtcctcgtcc tcgccgagct cgagctgcgt gcgcagctgg
     1981 gactcgacgg cgtccttgga ctcctcggag ctgatctgga tcgaggccat gtagtcgatg
     2041 aggttctcga gctgctgctc ggtgagcggg ccgccaccgc cctcgcccca ggcaggcatg
     2101 ggcgagtagg ggcggccgta ctcgaggatg tagtacacct cgtcgcggtc gtagcggagc
     2161 agcacggtgt cgagggccgg tgcctcccag ttgacctggg cgacgaactc gttgtcggcg
     2221 tcgaggatgg tgtgggaggc cacaccgccc acgccctcgg ggccgtggca ggcgttgcac
     2281 tgggcaccct cggtgtagag ctcctcgccc cggctctcga agaccgatgc gaactgctcc
     2341 tcggcgccct cgtggcggcc gggctcgccc agccagtagg cgggcaggcc gacgccgacg
     2401 aggaccaggc cggcgaggcc ggcggtgagg gcccggtcga gcttcttggt ctcgagctcg
     2461 tcgtcgtcga ggtagggctt caggttggcg gccagctcga gctcgctgcc cacctccttg
     2521 cgcccggcgc ggacgttgaa cgccgcgtac acgagcaccg ccacgacggc gagcagggcg
     2581 atgacggtgc cgatcgcctc caccgtgctc gcggcgaaga tggctgtggt gaggtcagtc
     2641 atggtggggt tcggtcgctc ttctcgactc tgggccgggc tcggtgggga cggggctcag
     2701 tctcagtggg acgctgcgcc gatgcagttc gggccctcgg cctcctggcc ggtggtgttg
     2761 gtgccgatgg ccgggccctg gatgatggtg ccggtgtcga cggacaggac gccgccggag
     2821 accgacatgg cgaagcggtc gaggccacgg ggggccggac cgcccttctt ctcgccgacc
     2881 cggttgtaca tgctgccgtg gcagccgcac tcgaaccact gcgacgtgac gcactcgggc
     2941 acgcgacagc ccaggtgcgg gcacttctgg tagagggcga tgacgccctc ctccatgccg
     3001 gcgagctcgc cgggcgagta gacggcccgg gccttctcga gggcgctggt ggggtactcg
     3061 gtgagccaca tgcggccctc gggctggtac aggaagccgt tgttgtcgcg gatctgggag
     3121 atcacgtcgc tgacgttgcc gaccgagatg accgagccga agccaccgcc gagctggggc
     3181 cacaggaagg cgatgcaggc ggcgccgaag ccggacaggc cgacgatgaa gaagccggtg
     3241 atgccgcggt tgaggaactg gcggcgggtg gcaccgaggg tgtcggggtc cggcggcacc
     3301 caggcggtgg gggccgacga gccggcggtg acgagctcct tgccggagcg gctgcgctcg
     3361 agcttggcgg cgagctcgag ctcgcgcccg gacgcggtgg ccttgccctc ctcgcggggc
     3421 gtgatggtgg cggccccggc atcgcgcgag cgcgtctccc gggacagcac gccgatggcc
     3481 ttctgggtct ggttgcggcg agccgcagcc aggaggacca cgccggccag caccacgagg
     3541 gcgacgacgg cgatgatggc gatggtggag gtgtccatgg tcagaacctt cttcctctgc
     3601 tcagagctcg aagaacaggc cggcgttcca ggggaacacg aagttgaagc ccgggccccg
     3661 gaagaacgag ccgatgatga cgagcacggc ccaga
//
LOCUS       DATISU010000062         2712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_561947, whole genome shotgun sequence.
ACCESSION   DATISU010000062 DATISU010000000
VERSION     DATISU010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561947"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VLR27_02765"
     CDS             complement(<1..280)
                     /locus_tag="VLR27_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HSP02394.1"
                     /translation="MPDLDDRAAIHDLVVHFYREVAFDDLLGPVFGEVAEVDWAQHIP
                     RLIGYWCRVLLGDPSYDGRILKAHERVDAQQPFDAELFDRWYRLFVESV"
     gene            complement(284..571)
                     /locus_tag="VLR27_02770"
     CDS             complement(284..571)
                     /locus_tag="VLR27_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02395.1"
                     /translation="MPAETHSHSEPVTADDVLELLATVVHDGDGLDPGTPVDALGLDD
                     DLALLSFWDVVAEEFGERTLGEPDIEELAGCRTAIELAETVARSFAAERTA"
     gene            615..743
                     /locus_tag="VLR27_02775"
     CDS             615..743
                     /locus_tag="VLR27_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02396.1"
                     /translation="MTRFLWILAGLGCVALGGIAVVAWHVPTREHVVEAGRTGRGW"
     gene            756..1511
                     /locus_tag="VLR27_02780"
     CDS             756..1511
                     /locus_tag="VLR27_02780"
                     /inference="COORDINATES: protein motif:HMM:NF020873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HSP02397.1"
                     /translation="MADVTLPVGLVEARRTPVFDFESLPAPLATSHRTTVWATLHVEE
                     GDVDYSDLEGDEPRHERLEAGDSIVIPPDVLHRVDPSTDARFHLQFHREPDAGMVPEL
                     HPEPPPSPRAAGPWEHRGRDLDDPDEIFEMVTRQYAVVVQDDILEPYFSAGGDFIDWQ
                     ALIGSVADFWNHALLYAPDYPVDPIERHREVHEHRALTPEALDRWLEIFHETIDTGWA
                     GPNAERAKKRGTGVAWAMAQRLLGKGAWRPGGG"
     gene            1582..>2712
                     /locus_tag="VLR27_02785"
     CDS             1582..>2712
                     /locus_tag="VLR27_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HSP02398.1"
                     /translation="MALAPIQTLSVGDRSYRYRPIADIDHGDVAGLPYSLKVLLENLA
                     RHAGAATVSDDDVAALAAWDPQAAQPRELAFTPARILLQDFTGVPAVVDLAAMRDAIE
                     TLGGDASAVNPLVPVELVIDHSVVADVWASDVAFARNVEIEYERNRERYQFLRWGQQA
                     FDGLTVVPPGTGICHQVNLEHLARVVFVDDDGMAYPDTLVGTDSHTPMVNGLGVLGWG
                     VGGIEAEAAMLGQPISMLTPKVVGFELTGELPEGSTATDLVLTVTELLRGHGVVGKFV
                     EFHGPGVSSVSLENRATIGNMTPEYGATCAIFPIDAETLRYLRFTGRPDEQVALVEAY
                     AKAQGLWHDPTATARYSEHLSLDLSTVVPSLAGPARPQDRVPL"
BASE COUNT          403 a         1001 c          935 g          373 t
ORIGIN      
        1 cgaccgactc gacgaagagc cggtaccacc ggtcgaacag ctcagcgtcg aagggctgct
       61 gggcgtcgac ccgctcgtgg gccttcagga tgcggccgtc gtagctcggg tcacccagga
      121 gcacgcggca ccagtagccg atgagccgcg ggatgtgctg ggcccagtcg acctcggcga
      181 cctcaccgaa cacgggtccg agcagatcgt cgaaggcgac ctcccggtag aagtggacga
      241 ccaggtcgtg gatcgcggcg cggtcgtcca ggtccggcac cggctacgcc gtccgctcgg
      301 cggcgaagga ccgggcgacg gtctcggcca gctcgatcgc cgtccggcaa ccggccagct
      361 cctcgatgtc gggctcgccg agggtccgct caccgaactc ctcggccacc acgtcccaga
      421 acgacaggag ggcgaggtcg tcgtcgagcc cgagggcgtc gaccggtgtc cccgggtcca
      481 gcccgtcacc gtcgtgcacc accgtcgcga gcagctcgag cacgtcgtcg gccgtcaccg
      541 gctcgctgtg gctgtgcgtc tcggcgggca tggtcctgag cgtagtattt gcatcacctg
      601 ctagaacaac aaggatgacc cggttcctgt ggatcctcgc cggcctgggc tgcgtggcgc
      661 tcggcggcat cgccgtcgtc gcctggcacg tgccgacccg cgagcacgtc gtcgaggccg
      721 gccggaccgg acgtggatgg tgataggcct cgtgcatggc tgatgtgacg ctcccggtcg
      781 ggctggtcga ggcccgtcgc acgcccgtgt tcgacttcga gtccctgccg gcaccgctgg
      841 cgacgtcgca ccgcacgacg gtgtgggcca ccctgcacgt ggaggagggt gatgtcgact
      901 actcggacct ggagggtgac gagccccgcc acgagcgcct cgaggccggc gacagcatcg
      961 tcatcccgcc cgacgtgctc caccgcgtcg acccctcgac cgacgcgcgc ttccacctgc
     1021 agttccaccg ggagcccgac gccgggatgg tgcccgaact gcaccccgaa ccaccgccgt
     1081 cgccccgcgc cgccggaccg tgggagcacc ggggtcggga cctcgacgac cccgacgaga
     1141 tcttcgagat ggtcacccgc cagtacgccg tggtggtcca ggacgacatc ctcgagccgt
     1201 acttcagcgc cggtggcgac ttcatcgact ggcaggcgct catcggcagc gtcgccgact
     1261 tctggaacca cgccctgctg tacgcccccg actaccccgt cgacccgatc gaacggcacc
     1321 gcgaggtcca cgagcaccgg gcgctgacgc cggaggcgct cgaccggtgg ctcgagatct
     1381 tccacgagac catcgacacc ggttgggcgg gacccaacgc cgagcgggcc aagaagcgcg
     1441 gcaccggcgt ggcgtgggcg atggcgcagc ggctgctggg caagggcgcg tggcgcccgg
     1501 gcggcggctg aaccggcaca agcccacccg tcgagcacgg aacgggtgca caccggccgc
     1561 aggcgtggta gtgcaccagg catggccctc gctcccatcc agacgctctc ggtcggcgac
     1621 cgctcgtacc ggtaccggcc gatcgcagac atcgaccacg gcgacgtggc gggactgccg
     1681 tactcgctga aggtgctgct cgagaacctg gcgcgccacg cgggcgcggc cacggtctcc
     1741 gacgacgacg tcgccgccct cgcggcgtgg gacccgcagg ccgcgcagcc acgggagctg
     1801 gcgttcaccc cggcccgcat cctcctgcag gacttcaccg gcgtccccgc cgtggtcgac
     1861 ctggcggcga tgcgcgatgc catcgagacc ctcgggggcg atgcctcagc ggtgaacccg
     1921 ctggtgccgg tcgagctggt gatcgaccac tctgtggtcg ccgacgtgtg ggcgagcgac
     1981 gtcgccttcg cccgcaacgt ggagatcgag tacgagcgca accgcgagcg gtaccagttc
     2041 ctccgctggg gccagcaggc cttcgacggc ctcaccgtgg tcccacccgg caccggcatc
     2101 tgccaccagg tgaacctcga gcacctggcg cgcgtcgtct tcgtcgacga cgacggcatg
     2161 gcctaccccg acaccctcgt cggcaccgac tcgcacacgc cgatggtgaa cggcctcggg
     2221 gtgctcggct ggggcgtcgg cggcatcgag gccgaggcgg cgatgctcgg ccagccgatc
     2281 tcgatgctca ccccgaaggt ggtcggcttc gagctcaccg gcgagctccc ggagggctcg
     2341 accgccaccg acctcgtgct caccgtcacc gagctgctgc gagggcacgg cgtggtgggc
     2401 aagttcgtcg agttccacgg acccggcgtc tcgtcggtga gcctcgagaa ccgggcgacc
     2461 atcggcaaca tgacgcccga gtacggcgcc acctgcgcga tcttcccgat cgacgccgag
     2521 acgctgcgct acctccggtt cacgggccgg ccggacgagc aggtcgcgct ggtggaggcc
     2581 tacgcgaagg cgcagggcct gtggcacgac ccgaccgcca ccgcccggta ctccgagcac
     2641 ctgtccctcg acctgtcgac cgtggtgccg agcctcgccg gcccggcacg gccgcaggac
     2701 cgcgtgcccc tc
//
LOCUS       DATISU010000063         5542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_142767, whole genome shotgun sequence.
ACCESSION   DATISU010000063 DATISU010000000
VERSION     DATISU010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142767"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VLR27_02790"
     CDS             <1..279
                     /locus_tag="VLR27_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02399.1"
                     /translation="GLRPFTVGDTETYAASGSAIVYWDSGNPTPPNGNVPPVDLDEDP
                     HSDPRKDAGALLQKQRFYADGTIVDVAGGTPNWTFACPRHPDRDPGCS"
     gene            331..1524
                     /locus_tag="VLR27_02795"
     CDS             331..1524
                     /locus_tag="VLR27_02795"
                     /EC_number="3.1.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF027980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ca2+-dependent phosphoinositide-specific
                     phospholipase C"
                     /protein_id="HSP02400.1"
                     /translation="MRRHLALLLAACLLVAACGDDDDAAPDETGADDTTTTTEAPEDE
                     VRLNEIQVLGSHNSYHLRPEPDVRAGIAAVAGERMALELDYEHLPLTEQLEDHGIRQF
                     ELDVYADPEGGRFADRPALAVLGRPTESGEPALAEPGFKVLHQVDVDFRSTCLTLVAC
                     LTEIETWSSANPDHEPVMIMIETKQQSIPEALGDDLDPALGIPWTEVLTFDRALFDAL
                     EAEIESVFDRGSIITPDDVRGDAATLEEAVLAGDAWPTLDAAAGKVLLSLVDLGPARD
                     VYVGDAAGLEGRLLFTSSEEGRPDAAFLRIDDPITEADRIRAAVEAGYLVRTRTDVPG
                     VDAVTGDTTRRDAAFESGAHYLSTDYYVADESLGYTLVVDLPGDGPARCNPVNAPEGC
                     DLAGG"
     gene            1521..1814
                     /locus_tag="VLR27_02800"
     CDS             1521..1814
                     /locus_tag="VLR27_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HSP02401.1"
                     /translation="MNPSEQPVVRRAVTVTGRVQGVSFRYHTVQQAERLGVTGWVRNH
                     DDGSVRLEVQGPPSAVGELVRWLHQGPPHARVDGVQEDAVDVRDGDAGFRAVR"
     gene            complement(1854..4394)
                     /locus_tag="VLR27_02805"
     CDS             complement(1854..4394)
                     /locus_tag="VLR27_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSP02402.1"
                     /translation="MLRLSIRQLLAHKIRFVMTSFAVVLGVSFVVGSLVVTDTVRRSF
                     DTLMTEISAGVDLQVRSGAPFDEQSFTGNREPMPAELLDVVLATDGVEIAEGGVGGTA
                     QPLGTDGEPVTTTGAPLIGTSWSGTDRLNPVTLNLGERPDEPGEVAFDQDTFEDQGYE
                     LGDTVTVLLPGGQESFTVVGTATFGESNSLAGAVLTMFSVEEAQRVFDSEGLYDEIYI
                     GIESGADADEVQAALAEALPDDVEVVTGTQVSEEGADAFGEITDVFGNVLLGFAAVAL
                     FVSAFYIYNTFSIILGQRIKELALLRAIGASAAQIRRTVILEALLIGTLSSLIGIGTG
                     VLTAMGLRGLLNAGGFSLPPGALVLKASTIITALVIGIGVTMAASIIPARSAAKVSPI
                     TALRDGVESGGRSRRLRLAIGAALTGVGAVLVLLGLTVTDGAAPTLTALGFGAILVFL
                     GVANLSPLIAGPVARLLGAPIAKLFGEPGVLARANAARNPYRTASTASALVIGLALVT
                     MASVVGTSVKETFASKIDQSVMADYVISESSFAGFSPRLAETLAETPELDAVSGIRFG
                     RFQFEGSTRDLTAVNADGGDLVDIDVQSGGTLADLGADGIFVHEDPARDLGLEPGDEV
                     TLAFSATGEQTFTVVGIHADATFAGNYVISTEAHDANFTDVTDGVVMARAADEVPAEE
                     ARAAVETALAEFPQAEIDDRQEFLDAQEAQIDQILVTVNVLLLLAVVIAVLGIANTLA
                     LSVFERTRELGLLRAVGMSRRQTRRMVRWEAAIVSLFGAVLGVVVGVLFGIAATTAMP
                     DSFLDRLAIPTGTLAGLLVLAVVAGLLAAIAPARRAAKLDVLRAITTE"
     gene            complement(4396..5163)
                     /locus_tag="VLR27_02810"
     CDS             complement(4396..5163)
                     /locus_tag="VLR27_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP02403.1"
                     /translation="MNEHDAAPAGIAARAIDAVKVYGSGTTEVTALDHVDVDLEAGRF
                     TAIMGPSGSGKSTLMHCLAGLDTLTSGQAVVDGTDLSTLSEKQLTELRRDRIGFVFQA
                     FNLVPTLTARENIVLPTRLAGRSPNETRLDEVIAAVGLGDRLDHRPSELSGGQQQRVA
                     VARALAADPAIVFADEPTGNLDSATGAEILSFMRNATRELGQTIVMVTHDPASAAYAN
                     RVLFLADGRIVDDMADPTADRVLDRMKRFGDELHEAR"
     gene            complement(5249..>5542)
                     /locus_tag="VLR27_02815"
     CDS             complement(5249..>5542)
                     /locus_tag="VLR27_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP02404.1"
                     /translation="ALLAPSVTRRLIEEFGRATPAPTPAGDERLARLTEREREVLVLM
                     ARGLSNGEIAEQLYLGEATVKTHVGRVLMKLEVRDRVQAVVHAYDTGVVVPGA"
BASE COUNT          807 a         1944 c         2046 g          745 t
ORIGIN      
        1 ggcctgcgcc cgttcaccgt cggcgacacc gagacctacg cggccagcgg ctcggccatc
       61 gtctactggg actcgggcaa cccgacgccg cccaacggca acgtgccacc cgtcgacctc
      121 gacgaggacc cgcacagcga cccgcgcaag gacgccggtg ccctgctcca gaagcagcgg
      181 ttctacgccg acggcacgat cgtcgacgtc gccgggggca ccccgaactg gacctttgcc
      241 tgtcctcgcc atccagatcg ggatccgggc tgcagctgac gctgccgcta caccacgact
      301 gccagtttcc gactcggcta caccgtgggg atgcgtcgcc acctcgcgct cctgctcgcc
      361 gcctgcctgc tcgtcgctgc ctgcggggat gacgacgatg ccgctcccga cgagaccggc
      421 gccgacgaca ccacgacgac gaccgaggcg cccgaggacg aggtccgcct gaacgagatc
      481 caggtcctcg gctcgcacaa cagctaccac ctgcgtcccg agcccgacgt gcgggccggc
      541 atcgccgccg ttgccgggga gcgcatggcc ctcgagctcg actacgagca cctgcccctc
      601 accgagcagc tcgaggacca cggcatccgc cagttcgagc tcgacgtcta cgccgacccg
      661 gagggcggtc ggttcgccga ccggccggcg ctcgcggtgc tcggccgacc cacggagagc
      721 ggggagccgg cgctggccga gcccgggttc aaggtgctgc accaggtcga cgtcgacttc
      781 cgcagcacgt gcctgacctt ggtggcctgc ctcaccgaga tcgagacgtg gtcgtcggcc
      841 aaccccgacc acgagccggt gatgatcatg atcgagacca agcagcagtc gatccccgag
      901 gccctcggcg acgacctgga ccccgccctc ggcatcccgt ggaccgaggt cctcaccttc
      961 gaccgggcgc tgttcgatgc gctggaggcc gagatcgagt cggtcttcga ccggggctcg
     1021 atcatcacgc ccgacgacgt ccgcggtgac gccgccacgc tggaggaggc cgtgctggcc
     1081 ggcgacgcct ggcccacgct ggacgccgcc gccggcaagg tcctgctctc gctggtggac
     1141 ctcgggccgg cccgcgacgt ctacgtcggc gacgccgccg gcctcgaggg tcgcctgctg
     1201 ttcacgtcgt cggaggaggg ccggcccgat gcggcgttcc tgcgcatcga cgatccgatc
     1261 accgaggccg accgcatccg cgccgcggtc gaggccggct acctggtgcg cacccgcacc
     1321 gacgtgcccg gggtcgacgc ggtcaccggc gacaccaccc gccgggacgc cgcgttcgag
     1381 agcggcgccc actacctgtc caccgactac tacgtggccg acgagagcct cggctacacc
     1441 ctcgtcgtgg acctgcccgg cgacggcccg gcccggtgca acccggtcaa cgccccggag
     1501 ggctgcgacc tcgccggcgg atgaacccgt ccgagcagcc cgtcgtccgc cgtgccgtca
     1561 ccgtgacggg tcgggtgcag ggcgtgtcgt tccgctacca caccgtccag caggccgagc
     1621 gcctcggcgt caccggctgg gtgcgcaacc acgacgacgg ctcggtccga ctcgaggtcc
     1681 agggaccgcc gagcgcggtc ggcgagctgg tgcgctggct gcaccagggc ccgccccacg
     1741 cgcgggtcga cggcgtccag gaggacgccg tcgacgttcg cgatggggac gccggcttcc
     1801 gcgccgtgcg ctgacggccg cgctaccgca gcactgccgg ttccaggaga ccgctactcg
     1861 gtggtgatgg cccggagcac gtcgagcttc gccgcccgac gggccggggc gatggcggcc
     1921 agcaggccgg cgaccaccgc cagcacgagc aggccggcca gcgtcccggt ggggatggcg
     1981 aggcgatcga ggaacgagtc gggcatggcg gtcgtggcag cgatgccgaa cagcacgccg
     2041 acgaccacgc cgagcaccgc accgaacagc gacacgatcg ccgcctccca gcgcaccatg
     2101 cgccgggtct ggcgccggga catgcccacc gcccgcagga ggccgagctc ccgggtgcgc
     2161 tcgaacaccg acagcgccag ggtgttggcg atgccgagca cggcgatgac cacggccagc
     2221 aggagcagca cgttgacggt caccaggatc tggtcgatct gggcctcctg ggcgtcgagg
     2281 aactcctggc ggtcgtcgat ctcggcctgc gggaactccg ccagcgcggt ctcgaccgcg
     2341 gcgcgagcct cctcggcggg gacctcgtca gcggcccggg ccatgaccac gccgtcggtg
     2401 acgtcggtga agttggcgtc gtgggcctcg gtggagatca cgtagttccc ggcgaaggtg
     2461 gcgtctgcgt ggatgccgac caccgtgaac gtctgctcac cggtggcgct gaaggccagg
     2521 gtcacctcgt cgccgggctc gagaccgagg tcccgggccg ggtcctcgtg cacgaagatc
     2581 ccgtcggcgc cgaggtcggc cagggtcccg ccggactgga cgtcgatgtc gacgaggtcg
     2641 ccgccgtcgg cgttcacggc ggtgaggtcg cgggtcgacc cctcgaactg gaagcggccg
     2701 aaccggatgc ccgacaccgc gtccagctcc ggcgtctccg ccagggtctc ggccagtcgg
     2761 ggggagaagc cggcgaagga ggactcgctg atcacgtagt cggccatgac cgactggtcg
     2821 atcttcgacg cgaaggtctc cttcaccgac gtccccacga ccgacgccat ggtcacgagc
     2881 gccaagccga tgaccagcgc cgacgccgtg gatgcggtcc ggtacgggtt gcgggccgcg
     2941 ttggcccggg cgagcacgcc cggctcgccg aacagcttgg cgatgggcgc gcccagcagg
     3001 cgggcgaccg gcccggcgat gagcggcgac aggttcgcca cgccgaggaa caccaggatc
     3061 gcaccgaacc cgagggcggt cagcgtcggt gcggcgccgt cggtgacggt cagcccgagc
     3121 agcaccagca cggcgccgac gccggtgagc gcggcaccga tggccaggcg cagccgtcgc
     3181 gagcggccac cggactcgac cccgtcgcgc agggcggtga tcggggacac cttcgccgcc
     3241 gaccgggccg ggatgatcga ggcagccatc gtgacgccga tgccgatgac cagcgcggtg
     3301 atgatggtgg atgccttcag caccagggca ccgggcggga ggctgaaccc gccggcgttc
     3361 agcaggccac ggaggcccat ggcggtcagc accccggtgc cgatgccgat gagggacgag
     3421 agggtgccga tgagcagcgc ctcgaggatc accgtccggc ggatctgggc ggcggaggcg
     3481 ccgatggccc gcaggagggc gagctccttg atgcgctggc cgaggatgat cgagaacgtg
     3541 ttgtagatgt agaacgccga cacgaacagg gccacggcgg cgaagccgag cagcacgttg
     3601 ccgaagacgt cggtgatctc accgaaggcg tcggcgccct cctcgctcac ctgggtgccg
     3661 gtcaccacct cgacgtcgtc gggcagggcc tcggccaggg cggcctggac ctcgtcggcg
     3721 tcggcaccgg actcgatgcc gatgtagatc tcgtcgtaga ggccctcgct gtcgaagacc
     3781 cgctgggcct cctccaccga gaacatggtg agcacggccc ccgcgaggga gttgctctcg
     3841 ccgaaggtgg cggtgccgac cacggtgaag gactcctggc cgccgggcag caggacggtg
     3901 acggtgtcgc cgagctcgta gccctggtcc tcgaaggtgt cctggtcgaa ggcgacctcg
     3961 ccgggctcgt cgggacgctc gccgaggttg agggtgacgg ggttgagccg gtcggtcccg
     4021 ctccaggacg tgccgatgag cggcgcgccg gtggtggtga ccggttcgcc gtcggtgccg
     4081 aggggctggg cggtgccgcc gacgccgcct tcggcgatct cgacgccgtc ggtggccagc
     4141 accacgtcga gcagctcggc gggcatgggc tcgcggttgc cggtgaagga ctgctcgtcg
     4201 aagggggcac cggagcggac ctgcaggtcg acgccggcgc tgatctcggt catgagcgtg
     4261 tcgaagctgc gccggacggt gtcggtcacc acgaggctgc cgaccacgaa gctcacgccg
     4321 agcacgacgg cgaagctggt catgacgaag cggatcttgt gcgccaggag ctggcggatg
     4381 ctgaggcgga gcatgtcagc gcgcctcgtg cagctcgtcg ccgaagcgct tcatgcggtc
     4441 gagcacccgg tcggcggtcg ggtccgccat gtcgtcgacg atgcgaccgt cggccaggaa
     4501 caggacccgg ttggcgtacg ccgcggacgc cgggtcgtgg gtgaccatga cgatggtctg
     4561 gcccagctcc cgggtggcgt tgcgcatgaa cgacaggatc tcggccccgg tggcggagtc
     4621 gaggttgccg gtgggctcgt cggcgaacac gatcgccggg tcggcggcca gtgcccgggc
     4681 cacggcgacc cgctgctgct ggccgccgga gagctcggag ggtcggtggt cgaggcggtc
     4741 gcccaggccg acggcggcga tgacctcgtc cagtcgggtc tcgttcggcg accggccggc
     4801 gaggcgggtc ggcagcacga tgttctcccg tgcggtgagc gtcggcacca ggttgaacgc
     4861 ctggaacacg aagccgatgc ggtcgcggcg cagctcggtg agctgcttct ccgacagggt
     4921 cgacaggtcg gtgccgtcga cgacggcctg gccggaggtc agcgtgtcga ggccggcgag
     4981 gcagtgcatc agggtggact tgccggaccc ggacgggccc atgatggcgg tgaagcgacc
     5041 ggcctcgagg tcgacgtcga cgtggtcgag cgcggtgacc tcggtggtgc ccgagccgta
     5101 gaccttcacg gcgtcgatgg cgcgggcggc gatgcccgca ggagctgcgt cgtgttcgtt
     5161 catgaggtgg acggtacgga tcgcccgtgg ttctgtcgtc cggctggcgg atgatgcgcc
     5221 gtcgtcctgc gggatgatgc ggcaccggtc aggcgccggg caccaccaca ccggtgtcgt
     5281 aggcgtggac caccgcctgc acgcggtcgc gcacctcgag cttcatcagc acccggccga
     5341 cgtgggtctt caccgtggcc tcgccgaggt acagctgttc ggcgatctcg ccgttggaga
     5401 gcccccgcgc catcagcacc agcacctccc gctcgcgctc ggtgagccgg gcgaggcgct
     5461 cgtcgccggc gggggtcggc gccggggtgg cgcgcccgaa ctcctcgatg aggcggcggg
     5521 tcacgctggg ggcgagcagc gc
//
LOCUS       DATISU010000064        28961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_748030, whole genome shotgun sequence.
ACCESSION   DATISU010000064 DATISU010000000
VERSION     DATISU010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 28961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28961
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748030"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..743)
                     /locus_tag="VLR27_02820"
     CDS             complement(297..743)
                     /locus_tag="VLR27_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP02405.1"
                     /translation="MFIEIVAEVTDELVAAFAHLIPQLSRSSPPPGKDELVDIVMSPA
                     TDLFVATDDDGTILGTATLVTFRIPTGRRAWIEDVVVDDAARGLGVGGALTQAMVDRA
                     EELGCTTVDLTSRPTREAANRLYQREGFEVRDTNVYRRTLETTREL"
     gene            complement(754..2388)
                     /locus_tag="VLR27_02825"
     CDS             complement(754..2388)
                     /locus_tag="VLR27_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HSP02406.1"
                     /translation="MLRRRERLVPAAAFLVLAAVGVLVLVLLTTAVSDGRRALEDSVL
                     SEVQANARSQGARLTGQISPIAGFIEQLDLELEVGSAADRAEFQDIADILEDLGVRTG
                     FFVTDASGTVTQGVQIDEAAVGRRLERPGFEEALEQFEAGVPGTWLPMAPGLSTELPT
                     FATVLPISGEGDALRGTFVLESEVAIDSGFNTEIRELGRGDTGELMLIDRLGGVIAAS
                     NPSLVGQQLPETDLLTVEPGVDRADGQVRAVADIPAAGWRIAFTQDSDEFDEGLAGPL
                     QQVGLIIVVVFLAVGVVAFVGLARRLQAAREEQERLQRLSETQEEFISIVSHELRTPV
                     AGVLGFLQTTMDHWDAMTDTERFNALRRSASNARRLQGLTRDVLDSQAVESGRMSYAM
                     GDADLAEEVIVAVEAASALYPRLTIDTDLESPHVPVVVDVDRIQQVLTNLLDNAAKVS
                     PPNGTVTVRLRRDGDRATVSVSDQGPGLSSDLQDRVFDKFVRGRDTSVSGTGLGLYIT
                     RQILEAHDGDITVETGEAGGATFAFTLPVAAVEVPR"
     gene            complement(2372..4384)
                     /locus_tag="VLR27_02830"
     CDS             complement(2372..4384)
                     /locus_tag="VLR27_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP02407.1"
                     /translation="MAAAGADPTAADRSGSVPLLAVLGLVAIGSCVLLAVSALGSRRA
                     ALAEQEAATTAEREAGRIAIAVQAVEAVVEHLDPSLCDPRVASDSPGPDFGGTPSSQA
                     APEGARLICLPAGGTTHPAAAGIDFGDPAIRAALAQSRDRGTGALSAAPAGGAPPVLA
                     APIYRAGGELLLEPSDRTTSVRREELAGHAVAVLDIGALLDDTSGWEVVDAGVVLARS
                     GDVVMDDDRTVSADLAALDRRWSVITQVPDAGLWRPISAGVALLGLAGAVLLGLADRQ
                     RRRVAQDQARAMRRAEERAAAIRTLTGIVQHSQDLDEILPALAVQLSDELGLTGVSLA
                     VATATGAERAIFSHGERPDPAPRPRRNRVGQVPSGATVAIDLHRADRSIAVMRVVTGV
                     ELDARGLELLHIAGEMITSTIVTGRSIEQQQEAVNRLESLDELKTTFLGVASHELRTP
                     ATAISGLATLLATRWDALTEDDRRAFASRIATNADALNALVQDLLDFARLERGDLQLA
                     LAPVVLSDAVGGVLDRLDGVWGSHDVTRAIDPDIEVLGDANAIERIVTNLVSNAVKFS
                     PSDADVAVTVEERGGRARLVVDDSGPGVPLEEREKIFVRFFRGAGDSVVRTRGVGIGL
                     SVVQDYVAQMGGSVRVEDSPLGGARFVVELDVLDRSRVEERDAATT"
     gene            4460..4891
                     /locus_tag="VLR27_02835"
     CDS             4460..4891
                     /locus_tag="VLR27_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP02408.1"
                     /translation="MVSTSGAERRFCRSGMEPMMTLQDTVRVMIADDNFDMRLLVRAT
                     LGGDGRLDVVAEAEDGTTAVDAFRITRPDVSVLDYRMPGLDGLEAATQILAEDPDTKV
                     LLFSAYLTPEITEAADALGMRCLRKDLFMELPAAVVQLARG"
     gene            complement(4902..5255)
                     /locus_tag="VLR27_02840"
     CDS             complement(4902..5255)
                     /locus_tag="VLR27_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSP02409.1"
                     /translation="MAHRIVVVDNDPDALELVVLDLSLEGIEVVGTAQDGVTALRLVA
                     ELAPDVLVVDHRMPPGPNGIDVAAEVRASHPETAVVLYSNYQSNDLLARSAALGVRFV
                     PKGNLRTLRRAVQGA"
     gene            5320..6162
                     /locus_tag="VLR27_02845"
     CDS             5320..6162
                     /locus_tag="VLR27_02845"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HSP02410.1"
                     /translation="MELLRWHFRPELERPIVVVAFEGWNDAGDAASSAVRWFADRWDA
                     TPFADIDPEVFYDFTSTRPEVRIEDDTRVIDWPANEFSATSLPGSDRDLVLVHGVEPQ
                     LRWRTFADLVLEVAQAFDASMVVTLGALLAEVPHSRPVNVVGTAYDQGVVTRLGLAPS
                     QYQGPTGIVGVLHDRCRSADLESASLWATVPTYVPGAPSPKATLALVEKTAELLGEQV
                     ITTDLEIASASYERQINELVDDDEETQAYVAALEQRHDEALSTTEPAELVEEVERFLR
                     EFGG"
     gene            6155..6679
                     /locus_tag="VLR27_02850"
     CDS             6155..6679
                     /locus_tag="VLR27_02850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP02411.1"
                     /translation="MADPIVRRATEADLDGITRVYDQYIVDSAVSFDVEPWPRERRLA
                     WWEDHQGHDRLVVLVAEADGEVVGAAYSSWYRPKVAYEHSLETSVVLDGAWTGRGLGV
                     RLYGTLLGELAAAGAHRAYAVVTLPNEASVALHHRLGFRDVGIEDECGWKLGRWWSTL
                     TLEKRFDDRGPDRP"
     gene            6676..7746
                     /locus_tag="VLR27_02855"
     CDS             6676..7746
                     /locus_tag="VLR27_02855"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HSP02412.1"
                     /translation="MTSAPADVDEAVRNAHAWFEHNSGWAPPDEETLAEWVADGVCRC
                     PDECLVAPRDWCEHGLASWWLILRAIDEQVVLDHFGIGAGALLGEGGEARVFALDADR
                     VLRIPHGRTSTDDLEARRALLDAVAGASVVALPEVLEHRAVADRLVVVERRLPGRNAL
                     QVLGEDCADRAALVRHHLDVAASIAALPCPTDRFGELWGAAAITSDRFRSWAIARLEA
                     SLERGGNRFAHLDVTGLTDELCAALPTREPTSPVLVHLDAYLGNMLADGDRITAVLDF
                     GPMAIGGPAHLDPLSAVAYLAPEITPTANDDDRAVARAWASDRGLLDALAPAERWVAA
                     YWTFATDDEALQRWCRRILGVG"
     gene            7794..8552
                     /locus_tag="VLR27_02860"
     CDS             7794..8552
                     /locus_tag="VLR27_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HSP02413.1"
                     /translation="MTTLPRTLTTTYTTLTWSEDGRSVTKVRDPAHDARRRFRYELKV
                     NTLLRDRPPPVPTPRLLDHDIGRRSLTFEAIGAEPVGPKYATELATEDLDAILTLTER
                     LREFDPRRRWFRRLDSAQRLALAQRHGLLTTSQTAGLRAIARRAHTRYRFCHGDITAR
                     NVLRHADTGELTLIDWEWAGLYPDGYELAFLWYSLIDVPDGRERVEARVARPDAFLLS
                     ALLITLWHLQWYLPGPFREKHLRTKDELVERLGC"
     gene            complement(8556..9995)
                     /locus_tag="VLR27_02865"
     CDS             complement(8556..9995)
                     /locus_tag="VLR27_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02414.1"
                     /translation="MRRLTRCAASLVAVALLAACAGDDATVDRAGTSSTTPSSAVTTS
                     TTTTSPPTTTAATVAADTCPAVPERAEPNPDRPVYEVTMTADPDTGVVEGTQAVRFVP
                     DLPVDRVVLRLWANGPRPASVGTSIELGAVAEEGRSAPVDQPDPTTAVVELGRWVPAG
                     EPITVWVPWRLFVGGPVNDRISRQDGSLRLGSFLPLLAWEPGVGWSVDPPTSGFAEST
                     SSPTARWSVSIDAPDGFGVVATGTEVEPGRWEVDAARDVAVSVGRFTVSTATAMAPDP
                     VQVEVAVHDGIDDGAVYVDRIVAALESFAAWYGPYPWPAYRLSITPELSGGIEFPMHV
                     LQGPDTSGRTTPHEVAHMWFYGLVGNVQGRDPWLDEGLATWAEGRIEGTLPDMAARSL
                     PADGEGRADAPMTYWEDRSASYYRSIYVQPAAALHRLGDDVLVDCALAHYVATNAWTI
                     AAPEDLWRSLDATFADWRPVLAPAGLAPG"
     gene            complement(10003..10707)
                     /locus_tag="VLR27_02870"
     CDS             complement(10003..10707)
                     /locus_tag="VLR27_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HSP02415.1"
                     /translation="MVEAPGPATGDPDSGPTRQDLLRWAESLAGIAKTGLGFTDSLYE
                     RERYEEVLAIAADIRVAAGTDYDGDHYVTEWLKGVRDGVPGYQTPKVAIGAVVGNDAG
                     EILLVQRADSGVWLYPTGWADIGYSPAEVAVKEVKEETGIDCEVERLLMVLDGLRLGF
                     TTVPLYSIVFQCRATGGDLAAHPLETSDVGFFAEDALPSPLAGYDHWGGPVFQALRGE
                     AVPVQFDSVRPETWRS"
     gene            10806..12827
                     /locus_tag="VLR27_02875"
     CDS             10806..12827
                     /locus_tag="VLR27_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSP02416.1"
                     /translation="MADRTAFHDLDRYLDQPRVAGLALSPDGTRLVTSVSTLSSDRTR
                     YVQALWELDPAGDAPARRLTRSAKGEHAPAFLPDGTLLFTSARPGADKDATDEDPPAL
                     WAQPPGAGDARVIARRPGGIGGAVTATAADVVVVQSDTLPGAVSAADDQERRKARKDR
                     KVSAILHESYPIRFWDHDLGPGQVRLLLGRNLADRDDPVDLADLTPVPGRRFDETEVS
                     ITPDGRTVLAAAALPEGGGTRSTLIAIDTSSRTERTLLHEPGHEFGGATISPDGTKAI
                     VVRESDGDPREPLTFTILVLDLADGTTTELAPDWDRWASGFRWVPDGSAVLCVADEQG
                     AAPVFRIDLADGTVTRLTTDHGAYSDLQVSPDGTTAYAVRSAVDHAPAVVRFDARTPG
                     ESTPLPGPAEPPALPGTLDEVTATAADGTPLRAWLALPHDAGETPAPLLLWIHGGPLG
                     SWNAWSWRWNPWLAVAQGYAVLLPDPALSTGYGLDFIRRGWGRWGDAPYTDLLAVTDA
                     AEARDDVDETRTAAMGGSFGGYMANWVAGHTDRFDCIVTHASLWALESFGPSTDRADY
                     WRRELDEAMAAEWSPHRFVEHMTTPMLVIHGDKDYRVPIGEALNLWSELTRHHGRDDG
                     SLPHKLLYFPDENHWILTPQHAKVWYEVLLGFLDHHLRGGDWSVPDILR"
     gene            complement(12834..15113)
                     /locus_tag="VLR27_02880"
     CDS             complement(12834..15113)
                     /locus_tag="VLR27_02880"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF002895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HSP02417.1"
                     /translation="MDLGRDELAADTSLVGRSLCAAYADRADAFLTELFEAASDRIGG
                     VSLVALGGYGRRELSLQSDLDVLLLHDGYKEIGPVADAIWYPVWDQGVKLGHAVRTVK
                     EALELAGDDLDSATAMLQARHLAGDRALTATLVEEVEQLWTSKASRFLPQLSDRVQER
                     HARAGEVAFLLEPDLKEGRGGLRDVQSLRWACRAREMLWEGDDEALQDAYGVLLDARV
                     ELHRRTGRMGDVLVLEEQDGVAEALGVADADDLMRGLAEAARVISFRSDDTWRRVGMT
                     LRRGIGRFARRDRALAPGAVVRDGEVHLLGEIDTSDPVLPLRTAALAAGEDVPVHRAV
                     LDLLEACPPLPTPWPDEARDQLVHLLGSGRSAISHLESLDQHGLLTKVLPEWEAVRSK
                     PQRNAYHTFTVDRHLCEAAANAAALAGRVSRPDLLVVGTWLHDIGKGFPGDHTVVGME
                     IVADIGARMGFAPDDVAVLVAMVEHHLLLPDVATRRDLDDPEVIQWVADSVGSTLLLE
                     LLHALTEADSQATGPAAWGPWKQELVDLLVRRVEHVLAGGEVDDVTDTFPSEEQRALV
                     TSGRRVVRGVGDTLTVVDADRPGLFSRVTGTLALHGMDVLDANIVTFDGRALEVITVQ
                     SSFGPTFAWDKVLRDLERALDGRLALRARLAERAATYRRRAPSTVRSEPKVEFHLDAS
                     SRATVVEVHAPDSVGVLHRITAAIAEFDLDIVRARVQTLLDEVVDSFYLEAAGGGKVP
                     EDVLPELRRAILHAVDEPR"
     gene            complement(15187..16131)
                     /locus_tag="VLR27_02885"
     CDS             complement(15187..16131)
                     /locus_tag="VLR27_02885"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: protein motif:HMM:NF015370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HSP02418.1"
                     /translation="MPMNDAALTEDLAPVVAGLVDRHLANSKEWFPHRIVPWSRGEDL
                     GKDWEWDPNETELSPEVRSALIVDLLTEDNLPYYFETIYNLFGKFEPWGTWARRWTAE
                     EGRHAIIIRDYLSVTRAVDLDDLERGRMAQVSTGEVPHPETVQDGLAYVTMQELATKI
                     SHRNTGRLLEDKVGYQVMATVAGDEQLHHVFYRDAATAALELDPDGMVLAIDRQVRNF
                     AMPGTGIPDFAQHAKRIADAGIYDFRIHHDQILKPMVVDQWKLPELEGLSPEADAARD
                     KVLKHLDRVDKAATRMTARRERHAAQDEAAAESELASA"
     gene            complement(16218..17582)
                     /locus_tag="VLR27_02890"
     CDS             complement(16218..17582)
                     /locus_tag="VLR27_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSP02419.1"
                     /translation="MAERLLVIGGDAGGMAAASQARRQQPYMEIVALERGRWTSYSAC
                     GIPYLVAGDVASHKELIARWPEEFRDRQRIDVRMGHEAIGIDLDARKVEVRDHSRDRT
                     LQLGFDLLHIATGAHPIRPPLPGIDSPFVHGVQTLDDGIHLLEHAERGGCESAVVVGG
                     GYIGVEMAEAFLRRGMKVTLVEADEQVMRTMDPDMAAPIATALRQLGIDVRLGVKAEG
                     FEDGAVHTSAGTIKADIVVLGLGVAPNSGLAADAGVKTGVRGAIRVNRRQQTSAAGVY
                     AAGDCAESYHRVTQRQVHVALGTVANKQGRVAGLNLGGGYATFPGVIGTAITKVCDFE
                     VARTGVNQEEADRAGFEYETVKIDSTTRAGYFPGTQPITIKMLAERDTRRVLGAQIVG
                     REGAAKRIDVLATAITAEMTVDDVIDLDLAYAPPFSGVWDAVHIAARKGAEALDRGVA
                     DRLR"
     gene            17627..17812
                     /locus_tag="VLR27_02895"
     CDS             17627..17812
                     /locus_tag="VLR27_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02420.1"
                     /translation="MAVREDCRHYSTRSTPAGDVLQRCRLGVNEEMPFACPEDCLFFE
                     PRQITGTGWQRFDRPPE"
     gene            17893..18570
                     /locus_tag="VLR27_02900"
     CDS             17893..18570
                     /locus_tag="VLR27_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HSP02421.1"
                     /translation="MELAEVMRTTPATRTFTDEAVPDEVLHRILDHARFAPSGGNRQG
                     WHVIVVKDAETRSRIRELYQLSWREYWAHVKIGKVAFQPGETRFSQPWVDLEEARETP
                     APNEFADKMDTHPVLLIVCVALEALAVLDHGLPRQSIVGGGSIYPFCHNIMLKARDEG
                     LGGVMTTAICREEAAVADLLGIPDTHAVASLIALGHPQEMVTKLRRDQVEEFAVVDRF
                     DGEAFEV"
     gene            complement(18675..19346)
                     /locus_tag="VLR27_02905"
     CDS             complement(18675..19346)
                     /locus_tag="VLR27_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HSP02422.1"
                     /translation="MSEHGDRAAFLDRIRSRQGRPPGGGPHPPPPAPDVVPEVRYRTL
                     DDVDHDDPFALLPVFVAAARTAEATVDVVDDEVPAELLLRFVSDHDVRTAVTSREPEA
                     VALGGALAAAGVSVAPYDRETAAAADLAITSATHGIAATGSVVVDSASAGSRAVSLLP
                     KIHLCVLPITRLVASHADVLRGQQRPMPSGRVLITGPSRTGDIEQRLTLGAHGPVALH
                     IVVVR"
     gene            complement(19339..20766)
                     /locus_tag="VLR27_02910"
     CDS             complement(19339..20766)
                     /locus_tag="VLR27_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HSP02423.1"
                     /translation="MSNEWPLRSSTLRERTATAVADPVLRANVGAATDRFRTKRTSAL
                     ARVHDPDGLRSAARGVRGDILSDWAATLELLADNVQANGGHVCWAPTGADANDYIAAV
                     ARRIGARTVVKSKSMATEETGLNEALAAVGCDTVETDLGEWIIQLAEEHPSHIIAPAV
                     HKNRDQVADTFEAKAGMTERVTEPTELVAFARRELRRRFLEADLGITGANLGVAETGS
                     VVLVTNEGNARMVTSMPRVHIAVMGAERIARTWDQADLVLGLLAKSGTGQTLSSYTTV
                     LSGPRADDEDDGPDEFHLVILDNGRSDIAGTEFAEMLNCIRCGSCLNVCPVYHQTGGH
                     AYGWVYSGPMGAVLTPLLASQQTGHQPGEEHHAGELANASTLCGACMEACPVEIPLQD
                     LLLGLRRRNAEHAGLAERSMWRAWAAAWSRPGAYEASATVATKARRLAPLLGRLPGGS
                     RWTDGRDLPDLPARTFRQRWDAGDV"
     gene            complement(20763..21569)
                     /locus_tag="VLR27_02915"
     CDS             complement(20763..21569)
                     /locus_tag="VLR27_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HSP02424.1"
                     /translation="MSDLPDARLAHEVERVALFVTCVGELVEPDVPIAAVRVLRAAGC
                     DVDVPEGQTCCGQPAWNSGFTTDAAKVARTSLDALEGAVAGGADAVVVPAGSCATMVR
                     VYWPQLFSLAGDEAASERARVLGERVWELTELLDALPDDRLDLGPVAPCAAVYHRSCH
                     MLRELHVAEQPEALLDRVQGCDVAEWAAADQCCGFGGTFSVKLPEASVAMADEKLDVL
                     PEGTEVIIGADASCLMQLRTRAGARGMPVRTEHIAQVLERALPDDGGAGT"
     gene            complement(21584..22081)
                     /locus_tag="VLR27_02920"
     CDS             complement(21584..22081)
                     /locus_tag="VLR27_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02425.1"
                     /translation="MASDANPPVAAADEERLAAIADELADRVEEVVPGWIEGLVLTRV
                     RECGDVSAEAAAEAVVAAEAASAEVGPRMRRLLETDIDDQAANPLELLRRATRHAHDA
                     LARIGVPSVVRDDFARRSFPDDSYDLMPATWSDIHPDLHEIGLTWGAAKAFVHKARRR
                     AEGRT"
     gene            22100..22594
                     /locus_tag="VLR27_02925"
     CDS             22100..22594
                     /locus_tag="VLR27_02925"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HSP02426.1"
                     /translation="MQPERVEPGPGQESVWDYPRPPALDRSDHHVVVRFGGEVIADTH
                     RPIRVLETSQPPAYYLPAADVRTDLLRPDGRRTFCEWKGQASYWTVDTGQRVAEAAVW
                     SYPSPTPRFAELVDHFAFYAQQMDECTVDGEVVDPNEGSFYGGWITSKVVGPFKGAPG
                     TIHW"
     gene            22605..22895
                     /locus_tag="VLR27_02930"
     CDS             22605..22895
                     /locus_tag="VLR27_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02427.1"
                     /translation="MKIRAMSVFEHVVYHSACLDPSNPSKPTLEIEAVVREGDVDDGP
                     VLLPWADFVFMVGKPIADRCYREFADAGRIVEHLGVKHLAFPLWTAGEIIHR"
     gene            complement(22947..23732)
                     /gene="larE"
                     /locus_tag="VLR27_02935"
     CDS             complement(22947..23732)
                     /gene="larE"
                     /locus_tag="VLR27_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HSP02428.1"
                     /translation="MGDLAALREHLVELGEVVVAFSGGADSSFLAWVANDVLGPERAT
                     AVTAVSPSLAGAEHDDCRTLAAEWGLSWREVHTEEMANAAYQRNDVDRCFWCKDALME
                     AVEPIVATAGATVTLGVNLDDLDDHRPGQQAARQRGAVFPLVDAGFTKADVRAASREL
                     GLRTWDKPAAPCLASRLPYGIPVTLGRLRSVEQAEAALRAMGFAELRVRHHEELARIE
                     VPTDRIDDVVARRDEVVAAVQDAGYRWVTLDLGGLRSGNLNPT"
     gene            complement(23725..24669)
                     /locus_tag="VLR27_02940"
     CDS             complement(23725..24669)
                     /locus_tag="VLR27_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSP02429.1"
                     /translation="MSGPVTAADEAPAEGGCGETSPQIELTSSAEAEVGRFRVRRALP
                     RRARRTVGAWCFADHMGPATVTEERGLDVGPHPHIGLQTVTWLISGEALHRDSLGSEQ
                     VIAPGQLNLMTAGHGIAHSEEATGRYAGELQGIQLWVAQPEDTRHGDPAFEHHAELPR
                     IDVGAGTATVLVGELEGEPSPARSDTDHLGVDLELGAGQTDLALVPTHEHGVVVLEGR
                     LTVEGHEVEPGMLAYLGVGHDGCRITTEGPTRAMLLGGTPLGDELVMWWNYVGRSRDE
                     IIDAHRAWTDHDTGRFGDVASPLPRIDTGPPPWGRSDG"
     gene            complement(24761..25858)
                     /locus_tag="VLR27_02945"
     CDS             complement(24761..25858)
                     /locus_tag="VLR27_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSP02430.1"
                     /translation="MTSPRLLSTLLAGAVAGAAVAAPITLLLDEQPAPAPPATASSDA
                     DATPVSAPAGSGLDVNAVLDTARPAVVAIRTQVVQPTSAGPQRGEGAGSGFVIDPAGT
                     IVTNSHVVEGARSISVELDDGTELDAELVARDPARDLAVLSVDGDDLPALEIGDSDAL
                     GVGDPVVAIGNALGLDGDLTVTTGIVSAKDRDVPVTRDITLRGTIQTDAAINPGNSGG
                     PLVDAQGRVVGINSAGARGAQSVGFAIPITDAMPVIEALARGEVPTRPLLGVQTADLT
                     DAVRAETGIDREDGALVMDVTAGSGAENAGIRPGDVIVGVDGNDIQTSADLGDAIEEA
                     GSDEEVEVEIDRGGQSLTVTATIGSRPGSVA"
     gene            25957..26634
                     /locus_tag="VLR27_02950"
     CDS             25957..26634
                     /locus_tag="VLR27_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP02431.1"
                     /translation="MSRLLVVEDDDAIAVPLVRALEGQGYDVEHVIGGGPAVERVADG
                     GIDLVILDLTLPDHDGLDVCREVRAAGSDVQILMLTARTDELDLVVGLDAGADDYVTK
                     PFRLAELLARVRARLRAATQDVEQPVSVAGGVRVDHAAHRAHQDDRELELTPTEFDLL
                     ALLVREAGRAVPRDEIMAAVWGPGWYGSSRTLDVHISALRRKLGDDPNQPRLLTTVRS
                     VGFRFEP"
     gene            26634..27866
                     /locus_tag="VLR27_02955"
     CDS             26634..27866
                     /locus_tag="VLR27_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSP02432.1"
                     /translation="MRSRLLLSTVGIATLVALLLGVPMGALLDRLAYETARSQAERQA
                     VSVGLALEPALVSGRVPTPALLEAIVGDQEQLTLVTSDGRTITGGTVEGTAYSVAAPG
                     PVGTQLQLSISDDDVRREIRRSWTALAALALFGIGAAVVLALLQSRRLSEPLLRVQRQ
                     AERIGAGDFTAPAPHAGLPEIDAIASALDETSARIAGLVRAERSFSANASHQLRSALT
                     GLLLRLEHLAEHDDPEVRNEAQAALDQGDRLLATVEDLLRLARTGRAGEQRPVTLHAL
                     AGEHVEDWRARFASMGRRIELRGDPDVVALVSPGGMGQVLDVLIDNARAHGEGTVEVH
                     VGRHADHAEVVVRDEGRGIDEGVEVFARRVDRDGHGLGLALARTLAEAEGGSLELLSR
                     EPTAFRVRVPATSVVPPT"
     gene            27952..28779
                     /locus_tag="VLR27_02960"
     CDS             27952..28779
                     /locus_tag="VLR27_02960"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HSP02433.1"
                     /translation="MTNAHGTTTQTRNAANGDWKETMGRIGLVGRGVMYAVIGLLAIQ
                     LALGNDSQEASNSGAIEWIAQQPLGKFLLIALTISLLCLAAWRLLDAAVGDPVEGDEP
                     SDRAEYAIKGVVYAALAVGALSATISNWNGDSGSGGGSGDSQNQEAAATVLEWPAGQW
                     IVTAVGLGIIGYAIYMFKKHVMDEEFLQRLSTSKDWVEKLGRGGYAARSAIFLVIGWF
                     LTQAGLTHEAGETKGLSGALQEISGRSWGQLLLLAVAVGLLAFGLFSLAEAKYRRDA"
BASE COUNT         3989 a        10563 c        10406 g         4003 t
ORIGIN      
        1 cgacgacggc atggccacgc tcgacccggg aacggcgagg gcgacggcta cccccagggc
       61 cacgaccgga ggagcaaagc ggacaaatcg tgtggacatg cgacaggcat cggccatccc
      121 gtgacacacc cgcagacaca agaggacctt tctgactggc gcgcacgaac tagtgcgaac
      181 ggcgcagaac ggacacggcc tcgagccgct ctgggacgcg agaagagccg gcgatcacaa
      241 gaccgaggtc gtcactcgat gagccatcgg cccccagcac cggagccggc ttcgcgtcag
      301 agctctcgcg tcgtctccaa ggtccggcgg tagacgttcg tgtcccggac ctcgaagccc
      361 tcccgctggt agaggcggtt ggccgcctct cgggtggggc gggaagtcag gtcgacggtg
      421 gtgcagccca gctcctcggc tcggtcgacc atggcctggg tgagcgcacc acccacgccg
      481 agcccgcggg cggcgtcgtc gaccacgacg tcctcgatcc aggcccgtcg cccggtcggg
      541 atgcggaacg tgacgagggt ggcggtgccg aggatcgtgc cgtcgtcgtc ggtggccacg
      601 aagaggtccg tcgcggggga catcacgatg tccaccagct cgtccttgcc gggcggcggg
      661 ctggagcggg acagctgggg gatgaggtgc gcgaaggccg cgaccagctc gtcggtgacc
      721 tcggcgacga tctcgatgaa cacggcggcg aaactagcgg gggacctcga cggcggcgac
      781 gggcagggtg aaggcgaagg tcgcgccgcc tgcctctccg gtctcgacgg tgatgtcccc
      841 gtcgtgggcc tcgaggatct ggcgggtgat gtacaggccg aggccggttc cgctgacgct
      901 ggtgtcccga ccccgcacga acttgtcgaa gacgcggtcc tgcaggtccg acgacagccc
      961 ggggccctgg tccgacacgg acaccgtggc ccggtcgccg tctcgccgga gacggaccgt
     1021 caccgtgccg ttcggcgggg agaccttcgc cgcgttgtcg agcaggttgg tgagcacctg
     1081 ctggatgcgg tcgacgtcga cgaccaccgg cacgtgcggg gactccaggt cggtgtcgat
     1141 ggtgaggcgg gggtacagcg ccgacgcagc ctcgacggcc acgatgacct cttcagcgag
     1201 atcggcatcg cccatggcgt aggacatgcg gcccgactcg acggcctggg agtcgaggac
     1261 gtcccgggtg aggccctgga gccgccgggc gttgctcgcc gagcgccgca gcgcgttgaa
     1321 ccgctcggtg tcggtcattg catcccagtg gtccatcgtc gtctgtagga acccgagtac
     1381 gccggcgacc ggggtccgca gctcgtggga cacgatgctg atgaactcct cctgggtctc
     1441 gctcaggcgc tgcaggcgtt cttgctcctc ccgggccgcc tgcaggcgtc gggccaaccc
     1501 cacgaacgcc accacgccca cggccaggaa gacgacgacg atgatcaggc cgacctgctg
     1561 cagcggacca gccagcccct cgtcgaactc gtcgctgtcc tgggtgaacg cgatccgcca
     1621 gccggccgcc gggatgtcgg cgaccgcacg cacctgcccg tcggcccgat cgacacctgg
     1681 ctccacggtg agcaggtcgg tctctggcag ctgctggccc accagcgacg ggttgcttgc
     1741 cgcgatgacg ccaccgaggc ggtcgatgag catcagctcg cccgtgtcgc cccgtcccag
     1801 ctcccggatc tcggtgttga acccgctgtc gatggcgacc tcgctctcca ggacgaacgt
     1861 gccccgcagc gcatctccct ctcccgagat cggcagcacg gtcgcgaagg tcggcagctc
     1921 ggtgctgagc ccgggtgcca tcggcagcca cgtgcccggg acaccggctt cgaactgctc
     1981 gagggcctct tcgaagccgg gacgttccag ccggcgaccg accgccgcct cgtcgatctg
     2041 cacgccctgg gtcaccgtgc cgctggcatc ggtcacgaag aacccggtcc gcaccccgag
     2101 gtcctcgagg atgtcggcga tgtcctggaa ctcggcacgg tctgcggcac tgccgacctc
     2161 gagctcgagg tccagctgct cgatgaaacc ggcgatcgga ctgatctggc cggtgaggcg
     2221 cgccccctgg ctccgagcgt tcgcctggac ctcgctgagg acggagtcct cgagcgctcg
     2281 gcgtccgtcg gacacggctg tcgtcagcag cacgagcacc aggaccccca ccgccgccag
     2341 caccaggaac gcagctgcag ggaccagccg ctcacgtcgt cgcagcatct cgctcctcga
     2401 ctctcgaacg gtcgagcaca tcgagctcga ccacgaaccg ggccccaccg agggggctgt
     2461 cctccacgcg caccgaaccg cccatctgag cgacgtagtc ctggaccacc gacaacccga
     2521 tgccgacgcc acgcgtacgc acgacggagt caccggcacc tcggaagaag cgcacgaaga
     2581 tcttctcccg ctcctccagg ggtactcccg ggccggagtc gtccaccacc agcctggcgc
     2641 gaccgcctcg ctcctcgacg gtcaccgcca cgtcggcgtc ggacggtgag aacttcacgg
     2701 cgttggacac cagattcgtg acgatgcgct cgatcgcgtt cgcgtcgccg agcacctcga
     2761 tgtcgggatc gatcgcacgg gtcacgtcgt gggagcccca gacgccatcg aggcgatcga
     2821 gcacgccgcc gacggcgtcg gacagcacga ccggggcgag ggcgagctgg agatcgcctc
     2881 gctccaggcg ggcgaagtcc aacaggtcct gcaccagcgc gttgagcgcg tcggcgttgg
     2941 tggcgatccg gctggcgaag gcgcgtcggt cgtcctcggt gagcgcatcc cagcgggtgg
     3001 cgagcagcgt ggcgaggccg gagatggcgg tggccggggt gcgcagctcg tgggacgcca
     3061 cgccgaggaa cgtcgtcttc agctcgtcca gcgactcgag ccggttgacc gcctcctgct
     3121 gctgctcgat gctgcgtccc gtcacgatcg tggaggtgat catctcgccg gcgatgtgga
     3181 gcagctcgag gccgcgagcg tcgagctcga caccggtgac cacacgcatc acggcgatgg
     3241 agcggtccgc gcggtgcagg tcgatggcga ccgtggctcc cgacggcacc tgcccgaccc
     3301 ggttgcgtcg cggccgcggc gcgggatcgg gtcgttcgcc atgggagaag atggcccgct
     3361 cggccccggt cgccgtggcg acggcgagcg agacacccgt gaggcccagc tcgtcgctga
     3421 gctggaccgc cagcgccggc aagatctcgt cgagatcctg gctgtgctgc acgatgccgg
     3481 tcagggtgcg aatcgctgcc gcccgctcct ccgctcgtcg catcgccctc gcctgatcct
     3541 gcgcgacccg gcgccgctgc ctgtcggcga ggccgaggag gacagctccg gccagtccca
     3601 gcagcgcgac gcctgctgag atcggccgcc acaacccagc gtccgggacc tgcgtgatga
     3661 ccgaccaccg ccgatccagg gccgcgaggt cagctgacac cgtccggtca tcgtccatga
     3721 cgacgtcgcc ggatcgggcc agcacgactc cggcgtcgac gacctcccac ccggatgtgt
     3781 cgtccagcag tgccccgatg tcgaggacgg ccactgcgtg acctgcaagc tcctcacgac
     3841 ggacgctcgt ggtccggtcg gacggctcga gcaacagctc cccacctgcc cgatagatcg
     3901 gtgccgccag caccggtggt gcgccgccgg cgggcgctgc gctgagggct cccgtgcccc
     3961 ggtcgcgtga ctgcgccagc gcagcccgga tcgccgggtc accgaagtcg atgcctgcgg
     4021 cggccgggtg ggtggtcccg ccagcgggga ggcagatgag cctcgcaccc tccggcgcgg
     4081 cttggctgct cggcgtgccg ccgaagtccg gcccgggact gtcagacgcc acgcgggggt
     4141 cgcacaggga cgggtcgagg tgctcgacga cggcttcgac ggcttggacg gcgatggcga
     4201 tgcgaccggc ctcgcgctcg gcggtggtgg ctgcctcctg ctcggccagg gctgctcgtc
     4261 gggagccgag cgcgctcacg gcgaggagca cacagctgcc gatggccacc aagccgagga
     4321 cggcgaggag cggcacggag ccggagcggt cggctgcggt cggatccgca ccagcagcag
     4381 ccaccccacc tcccgtcccg ttccgatgcc aggcgtccga atccggctgc gaccgtcgcc
     4441 gccgtccagc gtgccacgta tggtgtcgac cagcggcgcc gaacggcgct tctgccgatc
     4501 cgggatggaa ccgatgatga ccctgcagga cacggtccgg gtgatgatcg ccgacgacaa
     4561 cttcgacatg cggctcctgg tccgcgccac cctgggcggg gacgggcggc tcgacgtcgt
     4621 cgccgaagcg gaggacggca ccactgcggt cgacgcgttc cgcatcacgc gccccgacgt
     4681 gagcgtcctc gactaccgga tgccgggcct ggacgggctc gaggctgcga cgcagatcct
     4741 cgccgaggac cccgacacca aggtgctgct gttctcggcg tacctgaccc cggagatcac
     4801 cgaggctgcc gacgcgctcg gcatgcgctg cctgcgcaag gacctgttca tggagctgcc
     4861 cgccgccgtc gtgcagctcg ctcggggctg atccccgagc ctcaggcgcc ctgcacggcg
     4921 cgccgcagcg tccgcaggtt gcccttgggc acgaaccgga cccccagcgc cgccgatcgg
     4981 gccagcaggt cgttcgactg gtagttgctg tacagcacca cggcggtctc cgggtgcgac
     5041 gcgcgcacct ccgcagccac gtcgatgccg ttcgggccgg gcggcatccg gtggtcgacg
     5101 acgagcacgt ccggggccag ctcggcgacc aggcgcaggg cggtgacccc gtcctgggcg
     5161 gtgccgacga cctcgatccc ctccagggag aggtcgagca cgaccagctc gagggcgtcc
     5221 gggtcgttgt ccacgaccac gatgcggtgg gccacggccg gaccataggc ggcgccggtc
     5281 ccctcggtgc gggccaggcc tgccagtagc ctcggggccg tggaactcct ccgctggcac
     5341 ttccgtcccg agctggagcg acccatcgtg gtggtcgcct tcgaggggtg gaacgacgcc
     5401 ggcgacgccg cgtcctcggc ggtgcggtgg ttcgccgacc ggtgggacgc cacgccgttc
     5461 gccgacatcg acccggaggt gttctacgac ttcacctcca cccggcccga ggtccgcatc
     5521 gaggacgaca cccgggtcat cgactggccg gccaacgagt tcagcgccac ctccctgccc
     5581 gggtccgatc gtgacctcgt cctcgtccac ggcgtcgagc cccagctgcg ctggcgcacc
     5641 ttcgccgacc tcgtgctcga ggtggcccag gcgttcgacg cgtccatggt cgtcaccctc
     5701 ggtgcgctgc tcgccgaggt ccctcactcc cggccggtga acgtggtggg tacggcctac
     5761 gaccagggcg tcgtcacccg gctcggcctc gcgccgtcgc agtaccaggg ccccaccggc
     5821 atcgtcgggg tcctgcacga ccggtgccgc tccgccgacc tcgagtccgc gtcgctgtgg
     5881 gccaccgtcc ccacctacgt gcccggcgct ccatcgccga aggccacgct cgccctggtc
     5941 gagaagacgg ccgagctctt gggtgagcag gtgatcacca ccgatctgga gatcgcctcg
     6001 gcgtcctacg agcgccagat caacgagctg gtcgacgacg acgaggagac ccaggcctac
     6061 gtcgccgcgc tcgagcagcg ccacgacgag gcgctcagca cgaccgagcc cgccgagctc
     6121 gtcgaagagg tcgagcggtt cctgcgcgag ttcggtggct gacccgatcg tccggcgggc
     6181 cacggaggcc gatctcgacg gcatcacccg ggtgtacgac cagtacatcg tggactcggc
     6241 cgtgagcttc gacgtcgaac cgtggccgcg tgagcggcgc ctggcgtggt gggaggatca
     6301 ccagggtcac gaccggctgg tcgtcctcgt cgccgaggcc gacggcgagg tcgtcggtgc
     6361 ggcctactcg tcctggtacc ggccgaaggt cgcctacgag cactcgctcg agacctccgt
     6421 cgtcctcgac ggggcgtgga ccggccgggg tctcggcgtg cgcctctacg gcacgctgct
     6481 gggcgagctc gccgccgccg gggcacaccg ggcctacgcc gtggtgacac tgcccaacga
     6541 ggcatcggtc gccctgcacc accgcctcgg gttccgcgac gtcggcatcg aggacgagtg
     6601 cggctggaag ctgggccgct ggtggtcgac gctcaccctc gagaagcgct tcgacgaccg
     6661 cgggcccgat cgtccgtgac gtcggccccc gccgatgtcg acgaggcggt ccggaacgcg
     6721 cacgcctggt tcgagcacaa cagcgggtgg gcaccgccgg acgaggagac actcgccgag
     6781 tgggtcgccg acggggtctg ccgctgtccc gacgagtgcc tcgtcgcgcc ccgcgactgg
     6841 tgcgagcacg ggttggcgtc ctggtggctg atcctgcgag ccatcgacga gcaggtggtg
     6901 ctcgatcact tcggcatcgg tgctggcgcg ctgctcggcg aaggtggcga ggcccgggtg
     6961 ttcgcactcg acgccgatcg cgtcctgcgc atcccgcatg gacggacatc gacggacgac
     7021 ctcgaggcca ggcgcgcgct gctcgacgcc gtggccggcg caagcgtcgt tgcgctcccc
     7081 gaggtcctgg agcaccgcgc ggtcgcggac cgcctcgtcg tcgtcgagcg gcggctcccc
     7141 ggccgcaacg ccctgcaggt gctcggtgaa gactgcgccg accgcgcggc gctcgtgcga
     7201 caccacctcg acgtcgccgc atccatcgcg gcgctcccct gcccgacgga ccggttcggc
     7261 gagctctggg gcgcggcggc gatcacgagc gacaggttcc ggtcctgggc catcgctcgt
     7321 ctcgaggcct ccctcgagcg cggcggcaac cggttcgcgc acctcgacgt caccgggctc
     7381 accgacgagc tctgcgccgc gctccccacg cgggagccga cgtcgcccgt gctcgtccac
     7441 ctcgatgcgt acctcggcaa catgctcgct gacggcgatc gcatcaccgc cgtcctcgac
     7501 ttcggcccga tggccatcgg cggccccgcc cacctcgacc cgctctcggc agtcgcctac
     7561 ctcgcgccag agatcacgcc gacggcgaac gacgacgacc gggccgtcgc tcgtgcgtgg
     7621 gcgtccgacc ggggcctgct cgacgcgctc gccccggccg agcggtgggt cgccgcctac
     7681 tggacgttcg ccaccgacga cgaggcgctg cagcgctggt gccgccggat cctcggggtc
     7741 ggctgagcga gcgccaccac cgagaccctg cgctgtcggt accgtcacgg ctggtgacga
     7801 cactgccccg gacgttgacc accacgtaca cgacgttgac gtggtccgag gacggtcgct
     7861 cggtcaccaa ggtccgcgat cccgcccacg acgcccgccg ccgcttccgg tacgagctga
     7921 aggtgaacac cctcctccgg gaccgccccc cgcccgtgcc gaccccgcgc ctgctcgacc
     7981 acgacatcgg ccggcggagc ctcacgttcg aggcgatcgg cgccgagccc gtcggaccga
     8041 agtacgcgac cgagctcgcc accgaggatc tggacgcgat cctgacgctg accgagcggc
     8101 tgcgggagtt cgacccccgt cgtcggtggt tccgccggct cgactccgcg caacgcttgg
     8161 cgctggccca gcgccacggc ctgctcacga cgagccagac cgcgggtctc cgagccatcg
     8221 cgcgtcgggc ccacacccgc taccggttct gccatggcga catcacggcg cgcaacgtcc
     8281 tgcgccacgc cgacaccggt gagctcaccc tgatcgactg ggagtgggcc ggcctctacc
     8341 cggacggcta cgagctcgcg ttcctctggt acagcctcat cgacgtgccg gatggcaggg
     8401 agcgggtgga ggcgcgcgtc gcccgccccg acgccttcct cctgtcggcc ctgctcatca
     8461 cgctctggca cctccagtgg tacctgccgg gaccgttccg cgagaagcac ctccggacga
     8521 aggacgaact ggtcgagcga ctgggctgct gaccctcagc cgggcgcgag accagccggg
     8581 gcgaggaccg ggcgccagtc ggcgaaggtg gcgtcgagcg accgccacag gtcctccggc
     8641 gcggcgatcg tccaggcgtt ggtggcgacg tagtgggcga gggcgcagtc gacgagcaca
     8701 tcgtcgccca gccggtgcag cgcagccgcc ggttggacgt agatgctgcg gtagtagctg
     8761 gcggaccggt cctcccagta cgtcatcggc gcgtcggccc gaccttcgcc gtcggcgggg
     8821 agcgagcgcg ccgccatgtc ggggagcgtg ccctcgatcc gaccctcggc ccaggtggcc
     8881 agcccttcgt cgagccaggg gtcgcgaccc tgcacgttgc ccaccaaccc gtagaaccac
     8941 atgtgcgcga cctcgtgcgg cgtggtccga ccggacgtgt cggggccctg cagcacgtgc
     9001 atcgggaact cgatgccccc ggacagctcc ggggtgatgg aaagccggta cgccggccag
     9061 gggtaggggc cgtaccaggc ggcgaaggac tcgagcgcgg ccacgatccg gtccacgtag
     9121 accgcgccgt cgtcgatgcc gtcgtgcacg gcgacctcca cctggacggg gtccggggcc
     9181 atcgccgtcg ccgtcgacac ggtgaagcga ccgacgctga ccgcgacgtc acgggcggcg
     9241 tcgacctccc accggccggg ctcgacctcg gtgccggtgg ccaccacgcc gaagccgtcg
     9301 ggcgcgtcga tcgacaccga ccaacgggcc gtcggcgacg aggtcgactc ggcgaagccc
     9361 gacgtcggcg ggtcgacgga ccaccccacg cctggctccc aggcgagcag gggcaagaac
     9421 gaacccaagc gcagcgaccc gtcctggcgg gagatccggt cgttcaccgg tccgcccacg
     9481 aagagccgcc agggcaccca gaccgtgatc ggctcaccgg ccgggaccca tcggcccagc
     9541 tcgaccaccg cagtcgtggg atcgggttgg tcgaccggcg ccgagcggcc ctcctccgcc
     9601 acggcgccga gctcgatcga tgtgcccacc gatgcgggac gggggccgtt ggcccacagc
     9661 cgcagcacga cccggtcgac cggcaggtcg ggcacgaagc gcacggcctg ggtcccctcc
     9721 accacgcccg tgtccgggtc cgccgtcatg gtgacctcgt agacgggccg atccgggttc
     9781 ggctcggctc gctcgggaac agccgggcag gtgtcggctg cgacggtggc cgccgtcgtg
     9841 gtcggcgggc tggtggtcgt cgtgctcgtg gtcaccgcag acgatggcgt ggtggaggac
     9901 gtgcccgccc gatcgacggt cgcgtcgtcg ccggcgcatg cagcgaggag cgcgacggcc
     9961 accagcgatg cagcgcaccg ggtcagccgg cgcacggacg ggtcagctcc gccaggtctc
    10021 gggccggacc gagtcgaact gcaccggcac cgcctcgccc cggagggctt ggaacaccgg
    10081 tccaccccag tggtcgtagc cggcgagcgg tgagggcagg gcgtcctcgg cgaagaagcc
    10141 gacgtcgctc gtctcgagcg ggtgggcggc gagatcaccg ccggtcgctc ggcactggaa
    10201 cacgatcgag tagagcggca cggtggtgaa gcccagccgc aggccgtcga ggaccatgag
    10261 cagccgctcg acctcgcagt cgatgccggt ctcctccttg acctccttca ccgcgacctc
    10321 ggccggcgag tagccgatgt cggcccagcc ggtcgggtag agccagacgc ccgagtcggc
    10381 gcgctgcacg agcaggatct cgccggcgtc gttgccgacc acggcgccga tggccacctt
    10441 cggggtctgg tagccgggca cgccgtcgcg gacgcccttg agccactcgg tcacgtagtg
    10501 gtcgccgtcg tagtcggtgc ccgcggccac ccggatgtcg gcggcgatgg ccaggacctc
    10561 ctcgtagcgc tcgcgctcgt agaggctgtc ggtgaacccg agcccggtct tggcgatgcc
    10621 ggcgagcgat tcggcccagc ggagcaggtc ctggcgggtg ggtccggagt ccggatcgcc
    10681 ggtcgctggt cctggagcct ccaccatgac gtcgacgcta ctcagccggg ccggtgggac
    10741 cgccggcccg ccgtcgtgcg cgacactcgc cgggtcctga cctgccctcg accgggagcg
    10801 agccgatggc cgaccgcacc gccttccacg acctcgaccg ctacctcgac cagccccggg
    10861 tcgccggcct ggcgctgtcg ccggacggca cccgcctggt cacgtcggtg agcacgctgt
    10921 cgtcggaccg gacccgctac gtccaggcgc tgtgggagct ggaccccgcc ggcgatgcac
    10981 ccgcccgccg gctcaccagg tccgccaagg gcgagcacgc acccgccttc ctgcccgacg
    11041 gcaccctgct gttcacctcg gcgcgaccgg gcgcggacaa ggacgcgacc gacgaggacc
    11101 cgcccgcgct gtgggcccag ccccctggcg ccggcgacgc gcgcgtcatc gcccgccggc
    11161 ccggcggcat cggcggcgcg gtgacggcga ccgccgccga cgtggtggtc gtccagtcgg
    11221 acacgctgcc gggcgcggtg agcgcggccg acgaccagga gcggcgcaag gcccgcaagg
    11281 accgcaaggt ctcggcgatc ctgcacgagt cgtacccgat ccgcttctgg gaccacgacc
    11341 tcggtcccgg ccaggtccgg ctgctcctcg ggcggaacct cgccgatcgg gacgaccccg
    11401 tcgacctggc cgacctgacc cccgtcccgg gtcgccgctt cgacgagacc gaggtgtcca
    11461 tcacgccgga cgggcgcacc gtcctggccg ccgccgcgct gccggagggt ggcgggacca
    11521 ggtcgacgct catcgccatc gacacctcgt cgcgcaccga gcggacgctc ctgcacgagc
    11581 cgggccacga gttcggtggc gccacgatct cgcccgacgg gacgaaggcg atcgtggttc
    11641 gcgagtccga cggcgacccg cgggagccgc tcaccttcac catcctcgtg ctcgacctgg
    11701 ccgacgggac gaccaccgag ctggcgccgg actgggaccg ttgggccagc gggttccggt
    11761 gggtgccaga cggcagcgcc gtcctgtgcg tcgccgatga gcagggcgcg gccccggtgt
    11821 tccgcatcga cctcgccgat ggcaccgtca cccggctcac caccgatcac ggggcctaca
    11881 gcgacctgca ggtgagcccg gacggcacca cggcctacgc cgtgcgcagc gccgtcgacc
    11941 acgcacccgc cgtggtccgc ttcgatgccc gcacccccgg cgagtcgacc ccgctgcccg
    12001 gtccggccga gccgccggcg ctccccggca cgctggacga ggtgaccgcc acggcggcgg
    12061 acggcactcc cctgcgggcg tggctcgctc tcccccacga cgccggcgag acccctgctc
    12121 ccctgctgct gtggatccac ggtggcccgc tcggctcctg gaacgcgtgg tcgtggcggt
    12181 ggaacccgtg gctcgccgtc gcccagggct acgccgtgct gctccccgac ccggcgctgt
    12241 ccactggcta cggcctggac ttcatccgcc ggggctgggg ccgctggggc gacgccccct
    12301 acaccgacct gctcgccgtc accgacgccg ccgaggcccg cgacgacgtc gacgagacgc
    12361 gcaccgcggc catgggcggc agcttcggcg ggtacatggc caactgggtg gccgggcaca
    12421 ccgaccgctt cgattgcatc gtcacccacg ccagcctctg ggcgctcgag tcgttcggcc
    12481 cctcgaccga ccgggccgac tactggcggc gggagctgga cgaagccatg gcggccgagt
    12541 ggtcaccgca ccggttcgtg gagcacatga ccacgccgat gctggtgatc cacggcgaca
    12601 aggactaccg ggtgccgatc ggcgaggcgc tgaacctgtg gtccgagctg acccggcacc
    12661 acggtcgcga cgacgggtcg ctgccccaca agctgctgta cttccccgac gagaaccact
    12721 ggatcctgac cccgcagcac gccaaggtct ggtacgaggt gctcctcggc ttcctcgacc
    12781 accacctgcg cgggggcgac tggtcggtgc ccgacatcct ccgctgaggc gggctacctc
    12841 ggttcgtcga ccgcgtgcag gatcgcccgg cgcagctcgg gtaggacgtc ctcggggacc
    12901 ttcccgccgc cggcggcctc caggtagaac gagtccacca cctcgtcgag cagggtctgc
    12961 acccgggcgc ggacgatgtc gaggtcgaac tcggcgatcg ccgcggtgat gcggtgcagg
    13021 acgccgaccg agtccggcgc gtgcacctcg acgaccgtgg cccggctcga ggcatccagg
    13081 tggaactcca ccttcggctc gctgcgcacg gtgctcggcg cgcgccgccg gtacgtggcg
    13141 gcccgctcgg cgaggcgggc ccgcagcgcc agccgtccgt cgagtgcccg ctcgaggtcg
    13201 cgcagcacct tgtcccaggc gaaggtcggg ccgaaggagg actgcaccgt gatcacctcg
    13261 agcgcccggc cgtcgaaggt gacgatgttg gcgtcgagca cgtccatgcc gtgcagcgcc
    13321 agggtgccgg tgacccggga gaagagcccg gggcgatcgg cgtcgacgac ggtgagggtg
    13381 tcccccaccc cccggacgac ccgccggccg ctggtgacga gggcgcgctg ctcctcgctc
    13441 gggaaggtgt cggtgacgtc gtcgacctcg ccgcccgcga gcacgtgctc gacccggcgc
    13501 acgaggaggt cgaccagctc ctgcttccac ggtccccagg ccgccgggcc ggtggcctgg
    13561 gagtcggcct cggtgagcgc gtggagcagc tcgagcagca gggtgctgcc cacgctgtcg
    13621 gccacccact ggatgacctc ggggtcgtcg aggtcgcgcc gggtggccac gtcgggaagc
    13681 agcaggtggt gctccaccat ggcgacgagg acggccacgt cgtcgggggc gaagcccatc
    13741 cgggcgccga tgtcggcgac gatctccatg cccacgacgg tgtggtcgcc ggggaacccc
    13801 ttgccgatgt cgtgcagcca ggtgccgacc accagcaggt cggggcggct gacgcgaccg
    13861 gccagcgcgg cggcgttggc ggccgcctcg cacaggtgac ggtcgacggt gaacgtgtgg
    13921 taggcgttgc gctgcggctt cgaccgcacc gcctcccact ccggcagcac cttggtgagc
    13981 agcccgtgct ggtcgaggct ctcgaggtgg gagatggccg accggccgct gccgagcagg
    14041 tgcaccagct ggtcgcgcgc ctcgtccggc cagggcgtgg gcagcggcgg gcaggcttcg
    14101 agcaggtcga gcacggcgcg gtgaaccggc acgtcctcgc cggcggcaag cgctgcggtc
    14161 cgcagcggga gcaccgggtc ggacgtgtcg atctccccga gcaggtgcac ctcgccgtcg
    14221 cgcacgacgg ccccgggggc cagggcgcga tcgcggcggg cgaagcggcc gatgccgcgc
    14281 cgcagcgtca tcccgacccg ccgccaggtg tcgtccgagc ggaacgagat gacgcgagcg
    14341 gcctcggcca acccgcgcat gaggtcgtcg gcgtcggcca cgccgagcgc ctcggccaca
    14401 ccgtcctgct cctcgagcac cagcacgtca cccatgcgcc cggtgcggcg gtgcagctcc
    14461 acccgcgcat cgagcaggac gccgtacgcg tcctgcagcg cctcgtcgtc cccctcccag
    14521 agcatctccc gggcccggca cgcccaccgc agggactgga cgtcgcgcag tccgccgcga
    14581 ccctctttga ggtcgggttc gagcaggaac gcgacctcgc cggcccgggc gtggcgctcc
    14641 tgcacgcgat cggacagctg cggcaggaac cggctcgcct tcgaggtcca cagctgctcc
    14701 acctcctcca cgagggtggc cgtgagcgcc cgatcacccg cgaggtgccg ggcctgcagc
    14761 atcgccgtgg ccgagtcgag gtcgtccccg gccagctcga gcgcctcctt gacggtgcgc
    14821 accgcgtgac cgagcttcac cccctggtcc cacaccgggt accagatggc gtccgcgacc
    14881 ggaccgatct ccttgtaccc gtcgtgcagc agcagcacgt cgaggtcgga ctggaggctg
    14941 agctcacggc ggccgtagcc gccgagcgcg accaggctca cccccccgat gcgatccgag
    15001 gccgcctcga acagctcggt gaggaaggcg tcggcccggt cggcgtaggc ggcgcagagc
    15061 gagcggccga cgagcgacgt gtctgcggcg agctcgtcgc ggccgaggtc catcgggccg
    15121 agcctacggc tgccccgccg gcacccggat gcggccgttc aggtcccgtt cagttgcgac
    15181 aggtcctcag gcggaggcca gctccgattc ggcggcggcc tcgtcctggg cggcgtggcg
    15241 ctcgcgtcga gcagtcatcc gagtggcggc cttgtcgacg cggtcgaggt gcttgagcac
    15301 cttgtcgcgt gcggcgtcgg cctcgggcga cagaccctcg agctcgggca gcttccactg
    15361 gtcgacgacc atcggcttga ggatctggtc gtggtggatg cggaagtcgt agatgccggc
    15421 gtccgcgatg cgcttggcgt gctgggcgaa gtctgggatg ccggtgccgg gcatggcgaa
    15481 gttgcggacc tggcggtcga tggcgagcac catcccgtcg gggtcgagct ccagcgcagc
    15541 cgtggcggcg tcgcggtaga agacgtggtg cagctgctcg tcaccggcga cggtggccat
    15601 cacctggtag ccgaccttgt cctcgaggag ccggccggtg ttgcggtggc tgatcttggt
    15661 ggccagctcc tgcatggtca cgtaggccag gccgtcctgc acggtctcgg ggtgggggac
    15721 ctcaccggtg gagacctggg ccatgcggcc ccgctccagg tcgtcgagat cgaccgcccg
    15781 ggtcaccgac aggtagtcgc ggatgatgat cgcgtggcga ccctcctccg ccgtccagcg
    15841 gcgagcccac gtgccccacg gctcgaactt cccgaacagg ttgtagatcg tctcgaagta
    15901 gtacgggagg ttgtcctccg tgaggaggtc gacgatcagc gccgagcgga cctccgggga
    15961 gagctcggtc tcgttcggat cccactccca gtccttgccg aggtcctcgc cacggctcca
    16021 cggcacgatc cggtgcggga accactcctt ggagttggcc aggtgccggt cgacgagacc
    16081 ggcgaccacg ggcgcgaggt cttcggtgag ggcagcatcg ttcatcggca tgggactcac
    16141 cctaccgacc ggtcggtagg aacgaaaggg gcaaggacga gcgtcacagc tcgggctcta
    16201 cccgaccggg ccgggtctca gcgcaggcgg tccgcgacgc ctcggtcgag ggcctcggca
    16261 cccttgcggg cggcgatgtg caccgcatcc cagacgccgg agaacggcgg ggcgtaggcg
    16321 aggtccaggt cgatcacgtc gtcgacggtc atctcggcgg tgatggcggt ggccagcacg
    16381 tcgatgcgct tcgccgcgcc ctcccgtccc acgatctggg cgccgaggac ccggcgggtg
    16441 tcccgctcgg ccagcatctt gatggtgatc ggctgggtgc cggggaagta cccggcccgg
    16501 gtggtggagt cgatcttcac cgtctcgtac tcgaagccag cccggtcggc ctcctcctgg
    16561 ttgacgcccg tgcgggccac ctcgaagtcg cagaccttgg tgatggcggt gccgatcacg
    16621 ccggggaacg tggcgtaccc gccgccgagg ttcagcccgg cgacccggcc ctgcttgttg
    16681 gcgacggtgc cgagcgcgac gtgcacctgg cgctgggtca cgcggtggta gctctcggcg
    16741 cagtcgccgg cggcgtagac gcccgccgcc gaggtctgct gccggcggtt gacgcggatg
    16801 gcaccacgga caccggtctt cacgccggcg tcggcggcga ggccggagtt cggtgcgaca
    16861 ccgaggccga gcacgacgat gtcggccttg atcgtgccgg ccgaggtgtg cacggcgccg
    16921 tcctcgaagc cctcggcctt cacgcccagc cggacgtcga tccccagctg gcgcagcgcg
    16981 gtggcgatcg gcgcggccat gtccgggtcc atcgtgcgca tcacctgctc gtccgcctcg
    17041 accagcgtga ccttcatccc gcgacgcagg aacgcctcgg ccatctccac gccgatgtag
    17101 ccgccgccga ccaccacggc cgactcgcaa ccgccccgct cggcgtgctc gagcaggtgg
    17161 atcccgtcgt cgagggtctg cacgccgtgc acgaacgggc tgtcgatgcc ggggagcggg
    17221 ggtcggatcg gatgggcacc ggtggcgatg tggaggaggt cgaacccgag ctggagcgtg
    17281 cggtcgcggg agtggtcgcg gacctcgacc ttgcgggcgt cgaggtcgat gccgatggcc
    17341 tcgtgcccca tgcggacatc gatgcgctgg cggtcgcgga actcctcggg ccagcgggcg
    17401 atcagctcct tgtgggaggc gacgtcaccg gccacgaggt aggggatgcc gcaggccgag
    17461 tagctggtcc atcgcccgcg ctcgagggcg acgatctcca tgtagggctg ctgccggcgg
    17521 gcctgcgacg ccgcggccat gccgccggcg tcaccgccga tcaccaagag tcgttcggcc
    17581 atgggccggg agggtagttg cgcacctcgc gtgcgacact ggccgcgtgg ctgtacgaga
    17641 ggactgccgg cactactcga cccgttcgac accggcgggt gacgtgctcc agcgctgccg
    17701 cctgggcgtg aacgaggaga tgcccttcgc ctgccccgag gactgcctgt tcttcgagcc
    17761 ccgccagatc accggcacgg gctggcagcg cttcgaccgc cccccggagt agctctcgcc
    17821 tctcccctcc ccggttccgg gaaactggca gttcccctgt agcggcggct gcgcacctgg
    17881 catggtgggc ccatggagct cgccgaggtc atgcgcacca cgcccgccac ccggaccttc
    17941 accgacgagg ccgtccccga cgaggtgctg caccgcatcc tcgaccacgc ccgcttcgcc
    18001 ccgtcgggcg gcaaccgcca gggatggcac gtcatcgtcg tcaaggacgc cgagacccgg
    18061 agccgcatcc gcgagctgta ccagctgagc tggcgggagt actgggccca cgtgaagatc
    18121 ggcaaggtcg cgttccagcc cggcgagacc cgcttctccc agccgtgggt cgacctcgaa
    18181 gaggcccggg agacgccggc gccgaacgag ttcgccgaca agatggacac gcacccggtg
    18241 ctcctcatcg tgtgcgtcgc cctcgaggcg ctggccgtgc tcgaccacgg cctgccccgc
    18301 cagtcgatcg tgggcggcgg gtcgatctat cccttctgcc acaacatcat gctgaaggcg
    18361 cgcgacgagg gcctcggcgg cgtgatgacc acggcgatct gccgggagga ggccgcggtg
    18421 gccgacctgc tcggcatccc cgacacccac gccgtcgcct cgctgatcgc cctgggtcac
    18481 ccccaggaga tggtcaccaa gctgcgccgg gaccaggtcg aggagttcgc ggtcgtcgat
    18541 cgcttcgacg gtgaggcgtt cgaggtctga tgaaggccgg cgaggagccc gagcccgtca
    18601 ccgagggcgg cgacccggcg tgctgggccc acctcttcga cgacgacccg cgttccgtgg
    18661 tcgctgccag aacctcagcg gacgacgacg atgtggaggg cgacggggcc gtgggcgccg
    18721 agggtgaggc gctgctcgat gtcgccggtc ctcgacgggc cggtgatgag gacccggcct
    18781 gacggcatcg gacgctgctg accgcgcagc acgtcggcgt ggctggcgac cagccgggtg
    18841 atcggcagga cgcagaggtg gatcttcggc agcaggctga cggcacggct gccggcggat
    18901 gccgagtcga cgaccacgct gcccgtggcg gcgatgccgt gggtggccga cgtgatggcg
    18961 aggtcggcgg cggcggccgt ctcccggtcg tagggggcga ccgacacgcc ggccgcagcg
    19021 agcgcgccac cgagcgcaac ggcctcgggt tcccggctgg tcacggcggt gcgcacgtcg
    19081 tggtcgctca cgaagcggag caggagctcg gcggggacct cgtcgtccac gacgtcgacg
    19141 gtcgcctccg cagtgcgtgc ggcggccacg aacaccggga gcagcgcgaa cgggtcgtcg
    19201 tggtcgacgt cgtcgagggt gcggtaccgc acctcgggca ccacgtccgg tgccggcggc
    19261 ggcgggtggg gtccgccccc gggcggacgg ccctggcgag atcggatgcg gtcgaggaag
    19321 gcggcgcggt cgccgtgctc agacatcgcc tgcgtcccag cgctggcgga acgtgcgggc
    19381 tggcaggtcg ggcaggtcgc gtccgtcggt ccagcggctc ccgcccggga ggcgcccgag
    19441 cagcggcgcc agccgccgcg ccttcgtggc cacggtcgcc gacgcctcgt atgcccccgg
    19501 ccgggaccac gccgccgccc acgcccgcca catcgaccgc tcggcgaggc cggcgtgctc
    19561 ggcgttgcgg cgtcggaggc cgaggagcag gtcctgcagg gggatctcca ccgggcaggc
    19621 ctccatgcac gcaccgcaga gcgtcgacgc gttggcgagc tcgccggcgt ggtgctcctc
    19681 gcccggctgg tgcccggtct gctgcgacgc caggagcggc gtgagcaccg cccccatcgg
    19741 accggagtac acccagccat acgcatgccc gccggtctgg tggtacacgg ggcacacgtt
    19801 caggcacgac ccgcagcgga tgcagttcag catctcggcg aactcggtgc cggcgatgtc
    19861 ggagcggccg ttgtcgagga tgacgaggtg gaactcgtcg ggtccgtcgt cctcgtcgtc
    19921 ggcccggggc ccgctgagca cggtggtgta ggacgacagg gtctggccgg tgccggactt
    19981 ggccagcagg cccaacacca gatcggcctg gtcccaggtg cgggcgatgc gctcggcacc
    20041 catcaccgcg atgtgcaccc gcggcatgga ggtgaccatg cgggcgttgc cctcgttggt
    20101 gaccagcacg acgctgccgg tctcggccac gccgaggttg gcgccggtga tgccgaggtc
    20161 ggcctcgagg aaccgccgtc gcagctcgcg acgagcgaac gccaccagct cggtgggctc
    20221 ggtcacccgc tcggtcatcc cggccttggc ctcgaaggtg tcggcgacct ggtcgcggtt
    20281 cttgtgcacg gccggggcga tgatgtggct cgggtgctcc tccgcgagct ggatgatcca
    20341 ctcccccagg tcggtctcca ccgtgtcgca cccgacggcg gcgagcgcct cgttcaggcc
    20401 ggtctcctcg gtggccatcg acttggactt cacgaccgtg cgggcgccga tgcgccgggc
    20461 caccgcggcg atgtagtcgt tggcgtcggc gccggtgggc gcccagcaca cgtgcccacc
    20521 gttggcctgc acgttgtcgg cgagcagctc gagggtcgcg gcccagtcgg agaggatgtc
    20581 gccccgcacg ccacgggccg ctgagcgcag accgtcggga tcgtggacgc gtgccagcgc
    20641 tgacgtgcgc ttggtgcgga accgatcggt ggcggcaccg acgttggcgc gcagcaccgg
    20701 gtcggccacg gcagtcgccg tgcgctcgcg gagcgtggag ctgcgcagcg gccactcgtt
    20761 gctcatgtgc ccgctccccc gtcgtcgggc agcgcccgct ccagcacctg ggcgatgtgc
    20821 tcggtgcgca ccggcatgcc tcgcgcgccg gcccgggtgc gcagctgcat caggcaggag
    20881 gcgtcggcgc cgatgatgac ctcggttccc tcggggagga cgtcgagctt ctcgtcggcc
    20941 atggccaccg aggcctcggg cagcttcacg ctgaacgtgc caccgaagcc gcagcactgg
    21001 tcggccgccg cccactcggc cacgtcacag ccttggaccc ggtcgagcag cgcctcgggc
    21061 tgctcggcga cgtgcagctc ccgcagcatg tggcaggacc ggtggtagac ggcggcgcac
    21121 ggcgcgaccg gtccgaggtc gaggcgatcg tccggcagcg cgtcgagcag ctcggtgagc
    21181 tcccataccc gctcgcccag gacccgagcc cgctccgagg cggcctcgtc gccggccagc
    21241 gagaacagct ggggccagta cacccggacc atcgtggcgc aggagccggc cggcacgacg
    21301 acggcgtcgg caccgccggc gaccgccccc tccagcgcgt cgaggctcgt ccgggcgacc
    21361 ttggccgcat cggtcgtgaa ccccgagttc cacgccggct gcccgcagca cgtctggccc
    21421 tcggggacgt ccacgtcgca gcccgccgcc cgcagcaccc gcaccgcggc gatcggcacg
    21481 tcgggctcca ccagctcgcc gacgcacgtg acgaagagcg ccacgcgctc gacctcgtgg
    21541 gcgagacgtg cgtcggggag gtcggacatc ccccgatgtt cgctcatgtc cggccctcgg
    21601 cgcgccggcg ggccttgtgc acgaacgcct tggccgcacc ccaggtgagg ccgatctcgt
    21661 gcaggtccgg gtggatgtcg gaccacgtgg ccggcatgag gtcgtaggag tcgtccggga
    21721 aggaccgccg ggcgaagtcg tcccggacca cgctcggcac gccgatccgg gcgagcgcat
    21781 cgtgggcgtg gcgggtggcc cgacgcagca gctcgagggg gttggcggcc tggtcgtcga
    21841 tgtcggtctc cagcagccgg cgcatcctcg gcccgacctc ggcgctggcc gcctccgcag
    21901 cgaccaccgc ctccgccgcc gcctcggcgc tcacgtcgcc gcactcccgc acccgggtga
    21961 gcacgagccc ctcgatccag ccgggtacga cctcctcgac ccggtcggcc agctcgtcgg
    22021 cgatggcggc caggcgctcc tcgtcggcgg cggccacggg cgggttggca tcggaggcca
    22081 tgtgggtcaa cctacggacg tgcagcccga gcgcgtcgaa cccggacccg gccaggagtc
    22141 ggtgtgggac tacccccggc cgcccgcgct cgaccggagc gaccaccatg tcgtggtccg
    22201 cttcgggggt gaggtcatcg ccgacaccca ccgaccgatc cgcgtgctgg agaccagcca
    22261 gccgccggcg tactacctgc cggccgccga cgtccgcacc gacctgctcc ggccggacgg
    22321 ccggcgcacc ttctgcgagt ggaagggcca ggccagctac tggaccgtcg acaccgggca
    22381 gcgcgtcgcc gaggccgccg tgtggtcgta cccgagcccc acgccccgct tcgccgagct
    22441 ggtcgaccac ttcgcgttct acgcccagca gatggacgag tgcaccgtcg acggcgaggt
    22501 cgtcgacccc aacgagggtt cgttctacgg cgggtggatc acgtcgaagg tcgtcggccc
    22561 gttcaagggt gctccgggga cgatccactg gtgatggacc gcccgtgaag atccgcgcca
    22621 tgtcggtctt cgagcacgtc gtctaccact cggcctgcct cgacccgtcc aacccgtcca
    22681 agccgacact cgagatcgag gcggtggtgc gtgagggcga cgtcgacgac ggcccggtgc
    22741 tgctgccgtg ggcggacttc gtgttcatgg tgggcaagcc gatcgccgac cgctgctacc
    22801 gcgagttcgc cgacgccggc cggatcgtgg agcacctcgg ggtgaagcac ctcgccttcc
    22861 cgctgtggac cgccggcgag atcatccacc ggtgaccgtc gcggttccga aacgaccgga
    22921 tccggtcgaa gcaggaccac ggaagctcag gtcgggttca ggttccccga ccggagcccg
    22981 cccagatcca gggtgaccca ccggtagccg gcgtcctgca ccgcggcgac gacctcgtcc
    23041 cgccgtgcca ccacgtcgtc gatgcggtcg gtcggcacct cgatgcgggc cagctcctcg
    23101 tggtgccgga cgcgcagctc cgcgaagccc atcgcccgca gcgcggcctc ggcctgctcg
    23161 acggaccgca gccgcccgag cgtgaccggg atgccgtagg ggagccgcga cgcgaggcac
    23221 ggcgccgccg gcttgtccca cgtgcgcagg ccgagctcac gcgacgcggc gcgcacgtcg
    23281 gccttggtga accccgcatc cacgagcggg aagaccgcgc cccgctggcg cgccgcctgc
    23341 tgaccgggac ggtggtcgtc gaggtcgtcg aggttcacgc cgagcgtgac cgtggcgccg
    23401 gccgtggcca cgatgggctc gaccgcctcc atcagcgcgt ccttgcacca gaagcaccgg
    23461 tcgacgtcgt tgcgctggta ggcggcgttg gccatctcct cggtgtgcac ctcccgccag
    23521 ctcagccccc actccgcggc gagcgtgcgg cagtcgtcgt gctcggcacc ggccagcgac
    23581 ggggacaccg cggtcaccgc cgtggcccgc tcgggcccca gcacgtcgtt ggcgacccag
    23641 gcgaggaacg acgagtcggc gcccccggag aaggcgacga ccacctcgcc gagctcgacg
    23701 aggtgctccc gcagcgcggc gagatcaccc atcggagcga ccccagggcg gtggtccggt
    23761 gtcgatgcgg ggcaacggcg aggccacgtc gccgaagcgc ccggtgtcgt ggtcggtcca
    23821 ggcccggtgc gcgtcgatga tctcgtcgcg ggaccggccg acgtagttcc accacatcac
    23881 cagctcgtcc ccgaggggcg tgcccccgag cagcatcgcc cgcgtcggac cctcggtcgt
    23941 gatgcggcac ccgtcgtgcc cgacgcccag gtaggcgagc atccccggct ccacctcgtg
    24001 gccttcgacg gtcaaccggc cctccagcac gacgacgccg tgctcgtggg tcgggaccag
    24061 cgcgaggtcg gtctgtccgg caccgagctc gagatcgacg ccgaggtggt cggtgtcgct
    24121 ccgagccggt gagggctcgc cctcgagctc gccgacgagc accgtggcgg tgccggcgcc
    24181 gacgtcgatg cggggcagct cggcgtggtg ctcgaaggcg gggtcgccgt ggcgggtgtc
    24241 ctcgggctgg gccacccaga gctggatgcc ctgcagctcg ccggcgtagc ggccggtggc
    24301 ctcctcgctg tgggcgatgc cgtgcccggc ggtcatcagg ttcagctgcc cgggtgcgat
    24361 gacctgctcg gagccgaggg agtcccggtg gagggcctcg ccgctgatga gccacgtcac
    24421 ggtctggagg ccgatgtggg ggtggggtcc gacgtccagg ccccgttcct cggtcacggt
    24481 ggccgggccc atgtggtcgg cgaagcacca ggccccgacc gtgcggcggg cccgtcgagg
    24541 cagcgcgcga cgcacccgga accgaccgac ctcggcttcg gcgctcgacg tcagctcgat
    24601 ctggggcgac gtctcgccgc agcctccctc ggccggcgcc tcgtcggcgg cggtgacggg
    24661 gccgctcatg cgtcgacgac ccgaggggcc gcgtggtgac cgtggggccg catggcggcg
    24721 agggtaccgg cccaccgtcc cgggacggca gggccgcggc tcaggcgacc gagccgggcc
    24781 gggacccgat ggtggcggtc acggtcagcg actgcccacc gcgatcgatc tcgacctcga
    24841 cctcctcgtc ggagccagcc tcctcgatcg catcgccgag atcggcggag gtctggatgt
    24901 cgttcccgtc cacgccgacg atgacgtcgc cgggtcggat gccggcgttc tcggcgcccg
    24961 agccggcggt cacgtccatg acgagagcac cgtcctcgcg gtcgatgccc gtctcggcgc
    25021 gaaccgcatc ggtgagatcg gcggtctgga cgccgaggag gggtcgggtg ggcacctcac
    25081 cgcgggcgag cgcctcgatc accggcatcg cgtcggtgat cgggatggcg aagcccacgg
    25141 actgggcacc tcgggctccc gcgctgttga tcccgacgac gcgaccctgg gcgtcgacca
    25201 ggggtcctcc ggagttgccg ggattgatgg ccgcatcggt ctggatcgtg ccccggagcg
    25261 tgatgtcccg ggtcacgggc acgtcgcggt ccttggccga cacgatgccg gtcgtgacgg
    25321 tgaggtcgcc gtcgagaccc agcgcgttgc cgatggccac caccgggtcg ccgacgccga
    25381 gggcgtcgga gtccccgatc tcgagggcgg gcaggtcgtc gccgtccacg gacagcacgg
    25441 ccaggtcccg ggcggggtcc cgtgcgacca gctctgcgtc gagctcggtg ccgtcgtcga
    25501 gctcgacgga gatggaccgt gcgccctcga cgacgtggct gttggtgacg atggtgccgg
    25561 cggggtcgat cacgaagccg ctgcccgcgc cctcgccccg ctggggaccc gcggacgtgg
    25621 gctggaccac ctgcgtgcgg atggcgacga ccgctggccg ggcggtgtcg aggacggcgt
    25681 tcacgtccag gccgctgccg gcgggtgccg acacgggggt ggcgtcggcg tccgacgatg
    25741 ccgtggccgg gggcgccggc gccggttgct cgtcgaggag cagggtgatc ggcgcagcga
    25801 cggctgcgcc cgcgacagcg ccggccagca gcgtcgagag gaggcgtggt gaggtcatgt
    25861 gacgaacgct aggaacgccg ctcgggcccg acgcccagcg ccacgcgagg cttgtgtcaa
    25921 ggttctgcaa aggcgatcga ccggtaccct ccggccgtga gccgcctact cgtggtcgag
    25981 gacgacgatg cgatcgccgt gccgctcgta cgggcgctgg aggggcaggg ctacgacgtg
    26041 gagcacgtca tcggcggcgg accggcggtc gagcgggtgg ccgacggcgg catcgacctg
    26101 gtgatcctcg acctcaccct gccggaccac gacggcctcg acgtgtgccg cgaggtccgg
    26161 gccgccggca gcgacgtcca gatcctcatg ctcaccgccc gcaccgacga gctcgacctg
    26221 gtcgtgggcc tcgacgcggg cgccgacgac tacgtgacca agccgtttcg cctggccgag
    26281 ctcctcgccc gggtgcgcgc ccggctgcgc gcggccaccc aggacgtcga gcagcccgtg
    26341 tcggtcgccg gtggcgtccg ggtcgaccac gccgcccacc gggcccacca ggacgaccgc
    26401 gagctggagc tcactcccac ggagttcgac ctgctggcgc tgctcgtgcg ggaggcgggg
    26461 cgcgctgtcc cccgcgacga gatcatggcg gcggtgtggg gaccgggctg gtacgggtcg
    26521 tcccgcaccc tggacgtcca catctctgcg ctccgccgca agctcggcga cgaccccaac
    26581 caaccgcggc tgctcaccac cgtgcgcagc gtgggcttcc gcttcgagcc ctgatgcgat
    26641 cacggctgct gctgtcgacg gtcggcatcg ccaccctcgt cgcgctcctg ctcggcgtcc
    26701 cgatgggggc actgctcgac cggctggcct acgagacggc gcgctcgcag gcggagcgcc
    26761 aggcggtgag cgtcggcctg gcgctcgagc cggcgctcgt gtccggccgg gtcccgaccc
    26821 cggcgctgct ggaggcgatc gtcggcgacc aggagcagct caccctcgtc accagcgacg
    26881 gccgcaccat caccggcggc acggtcgagg gcacggccta cagcgtggcc gcgcccggcc
    26941 cggtgggcac gcagctccag ctgtcgatct ccgacgacga cgtgcgtcgc gagatccgcc
    27001 gctcgtggac ggcgttggcc gcgctcgcgc tcttcggcat cggggcggcc gtcgtgctcg
    27061 ccctcctcca gtcccgccgc ctcagcgagc ccctcctgcg agtgcaacgc caggccgagc
    27121 gcatcggtgc cggcgacttc accgccccgg cgccgcacgc cgggctcccc gagatcgacg
    27181 ccatcgcctc ggcgctcgac gagacctccg cccggatcgc cgggctggtg cgcgccgagc
    27241 ggtcgttctc ggccaacgcc agccatcagc tccgcagtgc cctcaccggg ctcctgctgc
    27301 ggctcgagca cctcgccgag cacgacgatc ccgaggtccg gaacgaggcg caggccgcgc
    27361 tcgatcaagg cgaccgcctc ctggccacgg tggaggacct cctccgcctc gcccgcaccg
    27421 gccgcgccgg cgagcaacga cccgtcacgc tccacgccct ggccggggag cacgtggagg
    27481 actggcgagc ccgcttcgcg tcgatgggac gcaggatcga gctgcgcggt gacccggatg
    27541 tggtcgccct ggtctcgccg ggcggcatgg gacaggtcct ggacgtgctc atcgacaacg
    27601 cccgtgccca cggcgagggc accgtggagg tgcacgtcgg ccggcacgcc gaccacgcgg
    27661 aggtcgtcgt ccgagatgaa ggacgtggca tcgacgaggg agtcgaggtc ttcgcccgcc
    27721 gggtcgaccg cgacggccac gggctcgggc tggcgttggc ccggacgctg gcggaggccg
    27781 agggcggttc gctggagctg ctgagccggg agcccacggc gttccgggtc cgggtgccgg
    27841 ccacctcggt ggtgcccccg acctgacggt ggggcgtcac cgggtggcgc gtcttggcgc
    27901 cgccgccgct ggagacagag accctgacaa caccagcgga cggggacaca gatgacgaac
    27961 gcacacggga ccacgaccca gaccaggaac gcagcgaacg gcgactggaa ggagaccatg
    28021 ggtcgcatcg gcctcgtggg ccgaggggtg atgtacgcgg tcatcggcct gctcgccatc
    28081 cagctcgccc tcggcaacga cagccaggag gcgtcgaaca gcggggcgat cgagtggatc
    28141 gcccagcagc ccctcgggaa gttcctcctc atcgccctga cgatctcgtt gctctgcctc
    28201 gccgcgtggc ggctgctgga tgccgccgtc ggcgatccgg tggagggcga cgaacccagc
    28261 gatcgcgccg agtacgccat caagggcgtc gtctacgccg ccctcgccgt cggtgcgcta
    28321 agcgccacca tctccaactg gaacggggac agcgggtccg gcggtggttc gggcgacagc
    28381 cagaaccagg aggccgcggc gacggtgctc gagtggccgg ccggccagtg gatcgtcacc
    28441 gccgtgggcc tcggcatcat cgggtacgcc atctacatgt tcaagaagca cgtcatggac
    28501 gaggagttcc tccagcggct ctcgacgagc aaggactggg tcgagaagct cgggcgtggc
    28561 ggctacgccg cccgatcggc gatcttcctc gtcatcggct ggttcctcac gcaggccggc
    28621 ctgacccacg aggccgggga gaccaagggc ctctccggtg cgctgcagga gatctccggc
    28681 cggagctggg gccagctcct gctgctcgcc gtcgctgtcg gcctgctcgc cttcggcctc
    28741 ttcagcctgg ccgaggccaa gtaccgccgc gacgcctgag cggcgtcgcg ggcccgcccc
    28801 agggcgcgtc cagctgacgc agcgcgccgc gggacctccc ttctctcacg tgcaccttga
    28861 caggtacccc tagggggtcc gagtatgagt ctccacaacc ccgagaggag ctccccagga
    28921 gcacgaacga gaccaccacc tccaccatcc ccgtcgccgg c
//
LOCUS       DATISU010000065         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_841550, whole genome shotgun sequence.
ACCESSION   DATISU010000065 DATISU010000000
VERSION     DATISU010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841550"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..556
                     /locus_tag="VLR27_02965"
     CDS             182..556
                     /locus_tag="VLR27_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02434.1"
                     /translation="MPDPAGSPRPLVVPWTLHAAFWLVLAESAIQGAFVVSRDDWGNV
                     GKALILLAFVAKVGFAQLARRLSAGGALGLLVFELVGILVAIGAEWAMGLRLLLVANV
                     VAVYALVLSSLHAFPTPELPRP"
     gene            553..1047
                     /locus_tag="VLR27_02970"
     CDS             553..1047
                     /locus_tag="VLR27_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02435.1"
                     /translation="MTSPSPTPQGLSYPTRTKVIIAVVLTVAIAAIVLAYVTTAEGGD
                     DSVSVSGADGLQDTGERDPSGVEALIPPRGSEILGQETIGIDLVPGWTGELTLLPGNG
                     VATTLPEDEIEVDGLNRIIYQPGPGKAIERLSGDYCLVATIWDQVEGREQTQRVENWC
                     FSAT"
     gene            complement(1066..>2191)
                     /gene="ligA"
                     /locus_tag="VLR27_02975"
     CDS             complement(1066..>2191)
                     /gene="ligA"
                     /locus_tag="VLR27_02975"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HSP02436.1"
                     /translation="FTSRAPRWAIAYKFPPEERTTTLLDIMVSVGRTGRATPYAVLEP
                     VFVGGANVERATLHNQDQVAAKDVRPGDTVIVRRAGDVIPEVVGPVLAARPEGLEPWV
                     FPTTCPCPVQSTLVRPEGESDTRCVHPECPFQVQGSIEHFASRGGMDIEGLGERTVAQ
                     LIELGLLHDIAGVYELDFDAILGLEGYGQTSVENLRSAIEASKDRPLRHLLVGLNIRH
                     LGPAGAEALARRFGHLDDIVAADADTLAAVDGVGPTIATSVRSWFDDPAHLAVVERLR
                     AAGVNLEGEAAPDVEQTLAGLSVVVTGTLEGYNREEVEEAIKSRGGKAPGSVSKKTTA
                     VVVGDGPGASKLTKAEELGIPVLDEAGFEHLLATGELPGQ"
BASE COUNT          291 a          792 c          804 g          304 t
ORIGIN      
        1 gtgacgtcgt cggccaggaa cacctgcgcg gacgaaccgg ccccgagcgg ggcgatcagt
       61 cggtaccggc cgcccaccac gcgaccgacc tggtcggaga gctgtgacat ggccactgcg
      121 ccacagtagg gaaccgcccc gccgccttgg tggaggggca ccggcgcgag actgggtcca
      181 aatgcccgat cccgccggct ccccccgccc cctcgtggtc ccctggacgc tgcacgccgc
      241 cttctggctg gtgctggccg agtccgcgat ccagggcgcc ttcgtggtca gccgcgacga
      301 ctggggcaac gtcggcaagg cgctgatcct gctggcgttc gtggccaagg tcggcttcgc
      361 ccagctggct cgccggctca gcgccggcgg cgccctcggg ttgctggtgt tcgagctcgt
      421 gggcatcctc gtcgccatcg gcgccgagtg ggcgatgggc ctgcgcctgc tgctggtcgc
      481 caacgtcgtc gccgtctacg ccctcgtgct gtcctcgctg cacgccttcc ccaccccgga
      541 gctcccccga ccatgaccag cccctccccc accccccagg gcctgtcgta ccccacccgc
      601 acgaaggtga tcatcgccgt ggtgctcacc gtggcgatcg ccgccatcgt cctcgcctac
      661 gtcaccaccg ccgagggcgg ggacgacagc gtcagcgtca gcggggccga cggcctgcag
      721 gacaccggcg agcgggaccc gtccggggtc gaggcgctga tcccgccgcg gggatcggag
      781 atcctcggcc aggagaccat cggcatcgac ctggtgccgg gctggaccgg cgagctcacc
      841 ctgctgcccg gcaacggcgt ggccaccacg ctgcccgagg acgagatcga ggtcgacggc
      901 ctgaaccgga tcatctacca gccgggcccg ggcaaggcca tcgagcgcct gagcggcgac
      961 tactgcctgg tcgccaccat ctgggaccag gtcgagggcc gcgagcagac ccagcgggtc
     1021 gagaactggt gcttctccgc gacctgacct gagttggcgg cccggctact ggccgggcag
     1081 ctcgccggtg gccaggaggt gctcgaaccc ggcctcgtcg aggacgggga tgcccagctc
     1141 ctcggccttg gtcagcttcg acgcaccggg accgtcaccg accaccacgg ccgtcgtctt
     1201 cttggagacc gagcccggcg ccttgccgcc ccgcgacttg atggcctcct cgacctcttc
     1261 gcggttgtag ccctcgagcg tgccggtgac cacgaccgag aggccggcca gggtctgctc
     1321 gacgtccggg gcggcctcgc cctccaggtt gacgccggcg gcgcgcagcc gctcgaccac
     1381 ggccaggtgg gcggggtcgt cgaaccagga ccggaccgag gtggcgatgg tggggccgac
     1441 gccgtcgacc gcggccaggg tgtcggcgtc ggcggcgacg atgtcgtcga ggtgaccgaa
     1501 ccggcgggcc agggcctcgg cgccggccgg gccgaggtgg cggatgttga gaccgacgag
     1561 caggtggcgc aggggccggt ccttgctggc ctcgatggcg ctgcgcaggt tctcgaccga
     1621 ggtctgcccg tagccctcca gcccgaggat ggcgtcgaag tccagctcgt agacgccggc
     1681 gatgtcgtgc aggagaccga gctcgatgag ctgggcgacg gtgcgctcgc ccaggccctc
     1741 gatgtccatg cccccgcggc tggcgaagtg ctcgatggac ccctgcacct ggaaggggca
     1801 ctcggggtgc acgcagcgcg tgtcggactc gccctcgggc cgcaccaggg tggactgcac
     1861 cgggcacggg caggtggtgg ggaagaccca gggctccaga ccctcgggcc gggcggcgag
     1921 caccgggccg accacctcgg ggatgacgtc gccggcgcgg cggacgatga cggtgtcgcc
     1981 ggggcggacg tccttggccg cgacctggtc ctggttgtgc agcgtggccc gctcgacgtt
     2041 ggccccgccg acgaacaccg gctcgagcac cgcgtacggc gtggcgcggc cggtgcgccc
     2101 gaccgagacc atgatgtcga gcagcgtggt ggtgcgctcc tcgggcggga acttgtaggc
     2161 gatggcccag cggggtgccc gggacgtgaa c
//
LOCUS       DATISU010000066         3607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_794581, whole genome shotgun sequence.
ACCESSION   DATISU010000066 DATISU010000000
VERSION     DATISU010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794581"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VLR27_02980"
     CDS             complement(<1..390)
                     /locus_tag="VLR27_02980"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSP02437.1"
                     /translation="MSGTGARDAIAVLCPHPDDEAIFCGGTIRALADAGHRVVIIAAT
                     SGDTAEGEPSPALRDLRRRELEAAGDILGAAAVHHLGFVDSGIGPVSPPGSFVRVPVE
                     QAAAELAAILDAEGVGSLVADGPEGIYG"
     gene            complement(387..1598)
                     /locus_tag="VLR27_02985"
     CDS             complement(387..1598)
                     /locus_tag="VLR27_02985"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP02438.1"
                     /translation="MSTEVTRARGWGVVVLVALGAAVAQAFGRFTYSVLLPAVRDDLG
                     VSNTVAGLLGTANVGAYLAGTLLIIRATATVRLMTIMRVGFTLSIAGLAIAGVAPNGA
                     VLAVGLVVMGLGGACIWIPSPAIAAAAVPPARRGTAVGMIGAGIGTGIVFAGRLSEAL
                     RDRWGVEAWQDVYRIETAIGLVVALAIVALLRPDAEPVPARTGSGLAVLRTMRGWVPL
                     TLAYAAFGFSYLLAITYLTARLEDDAGYSDGRAAGVFAAVGIATIIGGIALGAVADRI
                     GPRPSLVGGFTLFAAGTAGILTGQTAAVIGGGVAIGMMFGGLPSVIATYIVQASDHVT
                     YGPRYAVATLAFGIAQVTSPQVGGLLADLTGSFTVVFLLSGGVALLGAVASSMLPTPE
                     PESVHAEAIRT"
     gene            complement(1627..2157)
                     /locus_tag="VLR27_02990"
     CDS             complement(1627..2157)
                     /locus_tag="VLR27_02990"
                     /inference="COORDINATES: protein motif:HMM:NF023056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3253 domain-containing protein"
                     /protein_id="HSP02439.1"
                     /translation="MADREPERTEDGRYVVVDGRRWRASDPAIPEVLRQQLVDELMDA
                     RRAVGPAKRSGDDEAEVAARARVQAAKVALGERGQPWWEPPTDAGRRQRLEAAVLALA
                     HRRAPDKTICPSDAARAVGGEAWRDDMDLARDVVRALAADGRVEVLQRGEPVPADADW
                     KGPIRVRYSGGGAAPA"
     gene            complement(2265..3578)
                     /locus_tag="VLR27_02995"
     CDS             complement(2265..3578)
                     /locus_tag="VLR27_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSP02440.1"
                     /translation="MTKGTATTAEVLEVVAQVHDLCQRLDPAAIPLHEVPDAFDALAS
                     LERLGGGAVLRMAARYDEAGAWKRNGAKSAEDDIARKTGTPTNKARRNLSTSRRLKRQ
                     PKTDEAVRRGEVSPDQANEVSDGADASPDEEDELIRSARRDPLHELRKRSAAAKAKAD
                     RDREETRRRLHRAREMRRWNDPEGMGNLLLRLPADVMAEIDAALKPRVERAFADARDA
                     GRFERREALTADVARDLLLGGGDDAGATRRSQAVRPEKKVIALIDAPALNRGRVEGDE
                     VCEIAGVGPVSVSAVRALLSDAFLTLVFRNGEDVRNVTHLGRQVTARQRTAMEARGCS
                     CEREGCGGTYLLDIDHNEGWTLTHETRLDDLSWLCWHCHDLKTRNDLRLVGPPGRRRF
                     ETRKGKPWHAPPASGAPPGAGRPPDGTEAPARRPEPEQGTFTLAD"
BASE COUNT          452 a         1350 c         1350 g          455 t
ORIGIN      
        1 cccgtagatg ccctcgggcc cgtcggccac gagggaaccg accccctcgg catcgaggat
       61 ggcggccagc tcggccgccg cctgctccac cggcacccgc acgaacgatc cgggtggcga
      121 caccggcccg atcccggagt ccacgaagcc gaggtggtgc acggcggcgg cgccgaggat
      181 gtcgccggcg gcctcgagct cgcgccggcg caggtcgcgc agcgccgggc tgggctcgcc
      241 ctcggcggtg tcaccgctgg tcgcggcgat gatcacgacc cggtggccgg cgtcggccag
      301 ggcccggatg gtcccgccgc agaagatggc ctcgtcgtcg gggtgcgggc acagcacggc
      361 gatggcgtcg cgagcgccgg tcccgctcac gtccggatcg cctccgcgtg cacggattcc
      421 ggttccgggg tcggcagcat cgacgacgcc accgcgccga ggagcgccac gccgccggac
      481 aggaggaaca ccacggtgaa ggacccggtg aggtcggcga gcaggccacc gacctgggga
      541 gaggtgacct gggcgatgcc gaaggccagg gtggccacgg cgtagcgggg tccgtaggtg
      601 acgtggtcgc tggcctggac gatgtaggtg gcgatcacgg acggcaggcc gccgaacatc
      661 atcccgatgg cgacgccacc gccgatcacc gcggcggtct gcccggtgag gatcccggcc
      721 gtcccggccg cgaagagggt gaacccgccg acgagcgacg ggcgggggcc gatccggtcg
      781 gcgacggccc cgagggcgat gcccccgatg atggtggcga tgccgaccgc ggcgaacacg
      841 ccggcggccc ggccatcgct gtagccggcg tcgtcctcga gccgggcggt gaggtaggtg
      901 atggccagca ggtagctgaa cccgaaggcg gcgtaggcga gggtcagcgg cacccagccc
      961 cgcatggtgc gcagcacggc gaggccgctg ccggtgcggg ccgggaccgg ctcggcgtcg
     1021 ggccgcagga gggcgacgat ggccagggcg acgacgaggc cgatggcggt ctcgatccgg
     1081 tacacgtcct gccaggcctc gacgccccag cggtcgcgca gcgcctcgga cagccggccg
     1141 gcgaacacga tgcccgtccc gatgccggcg ccgatcatgc cgacggcagt gccccgccgg
     1201 gccggcggga cggcggcggc cgcgatggcc ggggacggga tccagatgca ggcgccgccg
     1261 aggcccatca cgaccagacc gacggcgagc accgccccgt tgggcgccac gccggcgatg
     1321 gcgagaccgg cgatcgacag ggtgaacccc acccgcatga tcgtcatgag ccggacggtg
     1381 gccgtggccc ggatgatcag cagcgtcccg gccaggtagg cgcccacgtt ggcggtgccg
     1441 agcaggccgg cgacggtgtt ggacacgccg aggtcgtctc gcaccgccgg cagcaggacg
     1501 ctgtaggtga agcggccgaa ggcctgggcc acggcagcgc cgagggcgac gaggacgacg
     1561 acgccccatc cccgtgctcg tgtcacctcc gtgctcatgt cccggccgcc atgccggcgg
     1621 agtctgtcag gcgggcgcgg ccccgccacc cgagtaccgg acgcggatcg ggcccttcca
     1681 gtcggcatcg gccgggacgg gctcgccccg ctggagcacc tcgacccggc cgtcggcggc
     1741 cagcgcccgg accacgtcgc gggcgagatc catgtcgtcg cgccacgcct caccgcccac
     1801 cgcccgggcc gcgtccgacg ggcagatcgt cttgtccggt gcccgccggt gggcgagggc
     1861 gaggacggcg gcctcgagcc gctggcgccg accagcgtcg gtcggcggct cccaccacgg
     1921 ctggccccgc tcccccagcg ccaccttggc cgcctgcacc cgggctcggg ccgcgacctc
     1981 ggcctcgtcg tcgccggacc gcttcgccgg gccgactgct cgccgggcgt ccatcagctc
     2041 gtcgaccagc tgctggcgca gcacctcggg gatggccggg tcgctggccc gccaccgccg
     2101 cccgtccacc acgacgtacc gcccgtcctc ggtccgctcg ggttcccggt ctgccatgcc
     2161 gccaccccta cccactgatc cgcccagccg cgcagcagcg cgcccgggac cacgacggtc
     2221 tccgggcacg ccggcggggg ctgggagctc gggcggacct tgcgtcagtc ggccagggtg
     2281 aacgtgccct gctcgggctc ggggcggcgg gcgggcgcct ccgtcccgtc gggcgggcga
     2341 cccgcgcccg gcggcgcacc gctcgccggc ggcgcatgcc acggcttgcc cttccgggtc
     2401 tcgaaccgcc gtcgtccggg cgggccgacc aaccggaggt cgttgcgggt cttgaggtcg
     2461 tggcagtgcc agcacagcca gctgaggtcg tcgagccggg tctcgtgcgt gagggtccag
     2521 ccctcgttgt ggtcgatgtc gaggaggtac gtgccaccgc acccttcccg ctcgcagctg
     2581 cagccccggg cctccatggc cgtgcgctgc cgggcggtga cctggcgacc gaggtgggtg
     2641 acgttgcgga cgtcctcccc gttccggaac acgagcgtga ggaaggcatc ggacagcagg
     2701 gcccgcacgg cggacaccga gacgggaccg acgccagcga tctcgcagac ctcgtcgccc
     2761 tccacccgac cccggttcag ggccggggca tcgatgagcg cgatcacctt cttctccggg
     2821 cgcaccgcct ggctccggcg ggtggcgccg gcgtcgtcac cgccgccgag cagcaggtcc
     2881 cgggcgacgt cggccgtgag ggcctcgcgc cgctcgaatc ggccggcgtc gcgagcgtcg
     2941 gcgaaggccc gctcgacccg gggcttgagg gcagcgtcga tctcggccat cacatcggcc
     3001 ggcaaccgca gcagcaggtt gcccatgccc tcgggatcgt tccagcgcct catctcccga
     3061 gcccggtgca ggcggcggcg ggtctcctcc cggtcccggt cggccttggc cttcgctgcg
     3121 gccgatcgct tgcgcagctc gtgcagcggg tcccggcggg ccgagcggat cagctcgtcc
     3181 tcctcgtcgg gcgacgcgtc ggccccatcg gacacctcgt tggcctggtc gggagacacc
     3241 tcgccccggc gcacggcctc gtcggtcttg ggctggcgct tcagccgcct gctggtggag
     3301 aggttgcgcc gggccttgtt cgtgggcgtg ccggtcttgc gggcgatgtc gtcctcggcg
     3361 ctcttggcac cgttgcgctt ccaggcgccg gcctcgtcgt agcgggccgc catgcgcagc
     3421 acggcgccgc caccgagccg ctcgagggac gccagggcat cgaacgcgtc gggcacctcg
     3481 tgcaggggga tggccgcagg gtcgagccgc tggcacaggt cgtgcacctg agccaccacc
     3541 tccaggacct ccgcagtcgt cgccgtcccc tttgtcatgg ttcgcaccat atcgaactgg
     3601 tgttcgc
//
LOCUS       DATISU010000067         3176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_515560, whole genome shotgun sequence.
ACCESSION   DATISU010000067 DATISU010000000
VERSION     DATISU010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515560"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VLR27_03000"
     CDS             complement(<1..981)
                     /locus_tag="VLR27_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HSP02441.1"
                     /translation="MARTDADIIGRDDVNDLDAILALTNTEKDEVIHTVQDNADALFT
                     WDYERSRPPLIKLYEKAKNSQWNGETDLDWSIEVDQEKLAAESPMTPTVEGWEALGAD
                     FSGTSFQSWTNKEWRQFQIESQNWSLSQFMHGEQGALICTAKIVETVPWIDAKYYAAT
                     QVMDEARHVEVFAKYLDTKLSGHYPINHHLKMLLDDIILDSRWDMTYLGMQIMVEGLA
                     LAAFGMAMQTTEDPLLKQLLRYVMSDEARHVAFGVLSLREYYEGLSDKEMMDRQEFAF
                     EAAVRMRDRFLQQEVWENMGIPVKEAISIFKQDDPELKNQDPFQQLLFSKI"
     gene            complement(1080..1202)
                     /locus_tag="VLR27_03005"
     CDS             complement(1080..1202)
                     /locus_tag="VLR27_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02442.1"
                     /translation="MGLFRSYAKFKVGKAAFDKARRWWRKRQRSKSGSRVEARR"
     gene            1273..>3176
                     /locus_tag="VLR27_03010"
     CDS             1273..>3176
                     /locus_tag="VLR27_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02443.1"
                     /translation="MHDDRDLAETRIRRTISDRLRPHIHRPGSPLEVTAWHVPGEPVP
                     VAEARDRVTGGGFDPFEIGGRWGKPWGTTWFRLQGRAPAEWSGETVEAIIDFSTGVGA
                     GFQSEGLLFRPAGGGPIRGIHPMHQAVPIDLVADEDGTIDVLVEAAANPVVATSYRPT
                     PLGDPATAGDELLYRLNRAELAVRNDEVWHLDLDLQVLGGLMRELALDDPRRHELLRA
                     IDAAVDVYDPTRPVETAADARGVLAPMLARDATDHAHRMSAIGHAHIDSAWLWPLRET
                     MRKCARTFSSAVTLMDEYPEYRFACSQAAQYAWIEEQQPELFARIAEKVADGRFVPVG
                     SMWVEADANLPSGESLARQLVWGRRWFLDHFGEGIEPGNRIVWIPDVFGYAGNLPQLM
                     AQAGCEFFLTQKLSWNQTNRFPHHTFWWEGIDGTRIFTHFPPADTYNGEVVPSELAYA
                     VRNFRDKGGSSRSMYLFGHGDGGGGPTREMVERARRVAGADGAGIDGLPRLAIESPAD
                     FFEAARDEYPDAPVWRGELYFEMHRGTYTTQARTKWGNRRGELALRELELWTAPGATG
                     AEAPLADLERLWKVLLLHQFHDIIPGSSIAWVHDDTEAAHAELLDEVDRLLTTALPPR
                     SGLHPAPPDGT"
BASE COUNT          453 a         1129 c         1081 g          513 t
ORIGIN      
        1 gatcttggag aacagcagct gctggaacgg gtcctggttc ttcagctcgg gatcgtcctg
       61 cttgaagatg gagatggcct ccttgaccgg gatgcccatg ttctcccaca cctcctgctg
      121 gaggaagcgg tcgcgcatgc ggaccgcggc ctcgaaggcg aactcctggc gatccatcat
      181 ctccttgtcg gagaggccct cgtagtactc gcgcagcgac agcacgccga aggccacgtg
      241 gcgggcctcg tcggacatga cgtagcggag gagctgcttg aggagcgggt cctccgtggt
      301 ctgcatggcc atgccgaacg cagcgagggc gaggccctcc accatgatct gcatgccgag
      361 gtaggtcatg tcccaccggc tgtcgaggat gatgtcgtcg agcagcatct tcaggtggtg
      421 gttgatcggg tagtggcccg acagcttcgt gtcgaggtac ttggcgaaga cctcgacgtg
      481 gcgggcctcg tccatcacct gggtggcggc gtagtacttg gcgtcgatcc acgggacggt
      541 ctcgacgatc ttggccgtgc agatgagcgc accctgctcg ccgtgcatga actgcgagag
      601 gctccagttc tggctctcga tctggaactg gcgccactcc ttgttggtcc agctctggaa
      661 cgacgtgccg ctgaagtcgg cgcccagcgc ctcccagccc tccaccgtcg gggtcatcgg
      721 gctctcggcg gcgagcttct cctggtcgac ctcgatcgac cagtccaggt cggtctcgcc
      781 gttccactgc gagttcttcg ccttctcgta cagcttgatg agcggcgggc gcgagcgctc
      841 gtagtcccac gtgaacagcg cgtcggcgtt gtcctggacc gtgtggatga cctcgtcctt
      901 ctccgtgttg gtgagggcga ggatcgcgtc gaggtcgttg acgtcgtcgc ggccgatgat
      961 gtcggcgtca gtgcgagcca tgtgggttcc cctggtgtgt tgcggtcagc gcgggccccg
     1021 atggggcacg tcgataataa cggtagttat cgacggcgcc ggtcgcaagg gggtgcccgt
     1081 cagcggcggg cctccaccct cgatccggac ttcgaccgct ggcgcttccg ccaccaccgg
     1141 cgggccttgt cgaaggcggc cttgcccacc ttgaacttgg cgtagctgcg gaagagtccc
     1201 atggtgctgc tccttgtctg acggtcgggt gcgctgggcc actacccgga ccggcggtcg
     1261 tacagtccgg gcgtgcacga cgaccgtgac ctcgccgaga cccggatccg ccgcacgatc
     1321 agcgaccggc tccgccccca catccaccgg ccgggaagcc cgctcgaggt gaccgcgtgg
     1381 cacgtgccgg gcgagccggt gccggtggcc gaggcccgcg accgggtgac cggcggcggg
     1441 ttcgacccct tcgagatcgg tggccggtgg ggcaagccgt gggggaccac gtggttccgc
     1501 ctgcagggtc gggcgccggc cgagtggtcg ggtgagaccg tggaggcgat catcgacttc
     1561 tccaccgggg tcggtgccgg cttccagtcc gaggggctgc tgttccgccc cgccggcggc
     1621 ggaccgatcc ggggcatcca cccgatgcac caggcggtgc cgatcgacct cgtggccgac
     1681 gaggacggca ccatcgacgt gctggtggag gccgccgcga accccgtcgt ggccacgtcg
     1741 taccggccga ccccgctcgg cgacccggcc accgcgggtg acgagctgct ctaccgcctc
     1801 aaccgcgccg agctggccgt gcgcaacgac gaggtctggc acctcgacct cgacctgcag
     1861 gtgctcggcg gcctcatgcg cgagctggcg ctcgacgacc cgcggcgcca cgagctgctc
     1921 cgggccatcg acgccgcggt cgacgtctac gacccgaccc gtcccgtcga gaccgccgcc
     1981 gacgcccgcg gggtcctggc gccgatgctg gcccgggacg ccaccgacca cgcccaccgc
     2041 atgtcggcga tcggccacgc ccacatcgac tcggcctggc tgtggcccct gcgcgagacc
     2101 atgcgcaagt gcgcccgcac cttctcctcc gcggtcacgc tgatggacga gtaccccgag
     2161 taccgcttcg cctgctccca ggccgcccag tacgcctgga tcgaggagca gcagcccgag
     2221 ctgttcgccc gcatcgccga gaaggtggcc gacggacgct tcgtcccggt cgggtccatg
     2281 tgggtggagg ccgacgccaa cctgccctcg ggcgagtccc tggcccgcca gctggtgtgg
     2341 ggccggcgct ggttcctcga ccacttcggc gagggcatcg agccggggaa ccgcatcgtg
     2401 tggatccccg acgtgttcgg ctacgccggc aacctgcccc agctgatggc ccaggccggg
     2461 tgtgagttct tcctcaccca gaagctgtcg tggaaccaga ccaaccggtt cccccatcac
     2521 acgttctggt gggagggcat cgacggcacc cggatcttca cccacttccc gccagccgac
     2581 acctacaacg gcgaggtcgt cccgtccgag ctggcgtacg cggtccggaa cttccgcgac
     2641 aagggcgggt cgagccggtc gatgtacctg ttcggccacg gtgacggagg tggcggcccg
     2701 acccgggaga tggtcgagcg cgcgaggcgg gtggccggcg cagacggcgc gggcatcgac
     2761 ggcctgcccc gcctcgccat cgagtcgccc gccgacttct tcgaggccgc tcgcgacgag
     2821 taccccgacg cacccgtgtg gcggggcgag ctgtacttcg agatgcaccg cggcacctac
     2881 accacccagg cccgcaccaa gtggggcaac cgccggggcg agctggcact gcgcgagctc
     2941 gagctgtgga ccgcacccgg ggccaccggc gccgaggccc cgctcgccga cctcgagcgg
     3001 ctgtggaagg tgctgctcct gcaccagttc cacgacatca tccccggcag ctcgatcgcc
     3061 tgggtgcacg acgacaccga ggccgcccac gccgagctgc tcgacgaggt cgaccgcctc
     3121 ctcaccaccg ccctcccacc ccgttctggc ctacatccag caccacctga tggcac
//
LOCUS       DATISU010000068         7629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_189515, whole genome shotgun sequence.
ACCESSION   DATISU010000068 DATISU010000000
VERSION     DATISU010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189515"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VLR27_03015"
     CDS             <1..260
                     /locus_tag="VLR27_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02444.1"
                     /translation="QPYFNYPPVQETIDATVREVIRCTKDDIRINTFMLDATQYLKTF
                     IEKITRMNGGRAFFTTPETLGDYVLVDFVEHKRAMRTNARR"
     gene            complement(362..748)
                     /locus_tag="VLR27_03020"
     CDS             complement(362..748)
                     /locus_tag="VLR27_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02445.1"
                     /translation="MIQDWEHHELRRSRRDLYHLSHEVALREAHAARQAGAAGWLAGH
                     DFRAVALLADAVETFAATSRTGSRPPDAAPIADDGINAVVLELADALVAELEQRRAEA
                     TGSAVAAFANLCHKVAWAGEVLRVRT"
     gene            987..1271
                     /locus_tag="VLR27_03025"
     CDS             987..1271
                     /locus_tag="VLR27_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HSP02446.1"
                     /translation="MSVPVDIGGQSETRVWQEYANCLGVDPDLFFPERGASTKEAKEV
                     CRGCVVREDCLEYALQNGEKFGIWGGMSERERRRIRRARAIARRAAVETA"
     gene            1413..1958
                     /locus_tag="VLR27_03030"
     CDS             1413..1958
                     /locus_tag="VLR27_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP02447.1"
                     /translation="MRRERREPAPGDGRWEGCDRIPTWEEVARHEGDFIYTVAYRLTG
                     DHDDAQDLVQEVLVRVQRGLRTYQPGSLRGWLSRITTNAFLDDVRRRSRRPAVALPDD
                     PDVVLPPSPDAAAAAEAERLPDHAQAALAGLAEEFRVPVVLADVVGLSYADIAEQLDI
                     PVGTVRSRIHRGRLALREALA"
     gene            1955..2746
                     /locus_tag="VLR27_03035"
     CDS             1955..2746
                     /locus_tag="VLR27_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSP02448.1"
                     /translation="MSAGTGGPDGHPDEELSALVDGELDPATEAWVAEHLDRCPACRQ
                     ATDELVQARSVLRGLPPVDATPVVEGFLARHRRMVRLGAGFVVVAGLVLAAIGATAAT
                     EHPRVVPDLAALTAGHVGQAHGEMAGMERRARSAYAAPPGLIGSAVQLSRHEVWDGTD
                     LAAVVYRDGAVDVSVYQQPGSLDWSGLPPGEVATVGDRAVWFGAGNPVVAVTQSGDLV
                     VTVVSDDRPAVLTAVAGMPEWRRRATWDRVHDACQRLVRVFALEG"
     gene            complement(2759..2995)
                     /locus_tag="VLR27_03040"
     CDS             complement(2759..2995)
                     /locus_tag="VLR27_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HSP02449.1"
                     /translation="MNDGSCRDHPPEVFFPSDGVGVTIAQRICATCPVREPCLEYALA
                     NRIDHGVWGATSERERRRILKARREPLEAYASGS"
     gene            3144..4349
                     /locus_tag="VLR27_03045"
     CDS             3144..4349
                     /locus_tag="VLR27_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSP02450.1"
                     /translation="MHPEEPTSGDGIPSTPPTRESMADLTAPRAWPRLDPPPHPGDRD
                     RAVGRSALVGGVVGGLVALLILGVAALWPTGGGGEDVATGDRPPMTTATGGVDVGAVL
                     SAVEPSVVSIEIRSSLADGQAGAGTGIILTQDGLVLTNAHVIAGLNQIQVGLFGGERA
                     PARLVGSFPESDIALIQIEGESDLVPAQLGSSASLQVGEEVIAIGNALNLGGRPTVTR
                     GIVSALDRSLSAEGITLSNLVQTDAAINPGNSGGPLVNAAGQVVGVNTAIIGDAQNIG
                     FSIEIDIVKPLIEEIRNGGGDVRGDQAFLGVNTTDVAAESPANRAEFDITVDEGAFVQ
                     SIVPDSAADEAGLEPGDVIVSVNGRATPDSASVGAIVRELQPGDEITISVLRAGAPVE
                     MTATLGQRG"
     gene            4427..4699
                     /locus_tag="VLR27_03050"
     CDS             4427..4699
                     /locus_tag="VLR27_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02451.1"
                     /translation="MSDVEMEEAPLPPVAHARLVYLGAVAPHWEIQSDWGDEAMIEAF
                     RSRVRARLVLLPPHDPQFRRNRERVNRDAERERIALDWDLGYSEDR"
     gene            4710..5666
                     /locus_tag="VLR27_03055"
     CDS             4710..5666
                     /locus_tag="VLR27_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSP02452.1"
                     /translation="MTVPVVGVDLGGTKIFGALVDPARTGLEAVLDDAKSPTPTGGVD
                     SVLDAVEAVVRRLDPEPSAVGIGTPGVVEPGTGRVLLAPNLSGFDRPVEVRRLLEDRL
                     GCPVSVGNDVNVAALGEARAGAGVGHDDVLAVWLGTGLGAGLVLDGHLRVGPHGLAGE
                     LGHSVVMPGGRRCGCGGLGHLEAYIGRRALESLARELHAAGRSTQLVELAGDGPMKSK
                     VFRAAYDAGDAVARELLDEGILLLAVAVVNVAVTVDISAVVVGGGLGERFADLVTEQL
                     ASALRTLGHAGIEPAVLPAALGDHAGALGAARLAVEAGRDAG"
     gene            5666..>7629
                     /gene="ppk1"
                     /locus_tag="VLR27_03060"
     CDS             5666..>7629
                     /gene="ppk1"
                     /locus_tag="VLR27_03060"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HSP02453.1"
                     /translation="MTPTDDLAPGVPPRRQRAAVPEAPDPSLTTDPGRYFNRELSWLD
                     FNARVLALADDPSVPLLERAKFLAIYVSNLDEFFQVRVAGLKDQVYAGVDQTSHDGRT
                     AGEQLLAIRNDVLALGLEQQRILLEEVLPGLAEERIHLTSWDDLDDDARKALYEVFEE
                     HIFPVLTPLAVDPGHPFPYISNLSLSLAVSVNDPETGETRFARVKVPSNLDRFVRIPG
                     SPTTFVPLEQVISAHLDELFPGMEIHESATFRVTRNADLTLEENEADDLLAAVEVELR
                     RRRFGRAVRLEVEAAMSDELRDLLVRELDIGDDDVYDVVGPLDLSGLWVLHRLDRHDL
                     KDPPWQGITQARLLKEDETPADFFAEMRRDDILVHHPYSSFHNTVEAFLAQAAADDRV
                     VAIKMTLYRTSGDSSIIRSLVRAAESGKQVAVLVELKARFDEARNIEWAKALEEVGVH
                     VVYGLVGLKTHTKTLLVVREERDGLHRYCHIGTGNYNPKTAMIYEDLGLFTTDPAIGT
                     DLSQLFNYLTGFGRAVDYEKLIVAPDHLRSRLEELIANEASYGPSGRIVAKMNALSDA
                     GMIDSLYEASQAGVSMDLVVRGICCLRPGVPGLSETIRVRSIVGRYLEHSRIYQFANG
                     NGRGSPAWFIGSPDLMGRNINRRVEALVPV"
BASE COUNT         1064 a         2875 c         2652 g         1038 t
ORIGIN      
        1 cccagccgta cttcaactac cccccggtcc aggagaccat cgacgccacc gtgcgcgagg
       61 tcatccgctg caccaaggac gacatccgca tcaacacctt catgctggac gccacccagt
      121 acctgaagac cttcatcgag aagatcaccc ggatgaacgg cgggcgggcg ttcttcacca
      181 cgcccgagac gctcggcgac tacgtgctcg tggacttcgt cgagcacaag cgggcgatgc
      241 gcaccaacgc ccgccgctga ggagccgagc cgacaggcga ggcgacccat gggcacggtg
      301 ccctacggcg gcgaagccgc cagaccgaat cgggcgcgga cgtgacctac cgccgcaggt
      361 cctacgtccg gacgcgcagc acctcgccgg cccaggccac cttgtggcac aggttggcga
      421 aggcggccac ggccgagccg gtggcctcgg cgcggcgctg ctccagctcg gcgacgagcg
      481 cgtcggccag ctcgagcacg accgcgttga tgccgtcgtc ggcgatggga gcggcatcgg
      541 ggggccggga gccggtgcgg gacgtggcgg cgaaggtctc cacggcgtcg gccagcagcg
      601 ccacggcccg gaagtcgtga ccggccagcc agccggcggc gcccgcctgc cgggcggcgt
      661 gggcctcacg gagcgcgacc tcgtggctca ggtggtacag gtcccggcgc gatcgacgca
      721 gctcgtggtg ctcccagtcc tggatcacgg tgccatggct accaggtggg tgcttgacac
      781 ggcgcctcgg ccgggggagg ggagcgccgg caccctcccg acctgcgccg tcaacatcaa
      841 ctggagacga atcccaggtc tcccttgcat ctcggcgcga tctcggcgat gatcacactg
      901 ttgtaattac acgtctggta tgggcgcccg ccgaggggga gactccggtg gcgccaccca
      961 gctgaacgaa cgagaggtcc tggcatatga gcgtccccgt ggacatcggt ggccagtccg
     1021 aaacccgagt gtggcaggag tacgcgaact gcctcggcgt cgatcccgac ctgttcttcc
     1081 ccgagcgggg cgcatccacc aaggaggcca aggaggtctg ccgcgggtgc gtggtgcgcg
     1141 aggactgcct ggagtacgcc ctgcagaacg gcgagaagtt cggcatctgg ggcggcatga
     1201 gcgagcgtga gcgccgccgg atccggcggg cccgggccat cgcccggcgc gccgcggtcg
     1261 agaccgcctg acccgcctca ccgccagcca cgcccgcgct cgcgggctgc acctgcccat
     1321 cgccacgccc cgcgccgcgt ccccctgcgg cacggggcgt gccgcgtccg gggccggcgg
     1381 gcgagccgac cctcgccggt agcctcgacg cggtgcgacg cgagcggcgg gaacctgctc
     1441 ccggcgacgg tcgttgggag gggtgtgacc gcatccccac gtgggaggag gtcgctcgcc
     1501 acgaaggcga cttcatctac accgtcgcct accgcctcac gggcgatcac gacgacgcgc
     1561 aggacctggt gcaggaggtg ctggtcaggg tgcaacgagg actgcggacc taccagcccg
     1621 gctcgctgcg cgggtggctg agccgaatca ccaccaacgc gttcctcgac gacgtccgcc
     1681 gccggtcccg ccggcccgcg gtcgccctgc ccgacgaccc cgacgtcgtc ctgccgccga
     1741 gcccggacgc cgccgcagcg gccgaggccg agcgcctccc ggaccacgcc caggcggcgc
     1801 tggccggtct ggccgaggag ttccgcgtcc cggtcgtcct cgccgacgtg gtcgggctgt
     1861 cctacgccga catcgccgag cagctcgaca tccccgtggg caccgtgcgc agccgcatcc
     1921 accgcggccg gctggcgctg cgggaggcgc tggcgtgagc gccggcaccg gcggcccgga
     1981 cgggcacccc gacgaagagc tgtcggccct cgtcgacggc gagctggacc cggccaccga
     2041 ggcctgggtc gccgagcacc tcgaccgctg cccggcgtgc cggcaggcca ccgatgagct
     2101 ggtgcaggcg cggtccgtgc tgcgcggcct gccgcccgtg gacgccacgc ccgtcgtcga
     2161 ggggttcctg gctcggcacc gccgcatggt gcgcctcggc gccggcttcg tggtggtggc
     2221 cggcctcgtg ctcgccgcca tcggggccac ggcggccacc gagcacccgc gcgtggtgcc
     2281 cgacctcgcc gccctcacgg ccgggcacgt cgggcaggcc cacggggaga tggcgggcat
     2341 ggagcgccgc gcccgctccg cctacgccgc gccgcccggg ctgatcggtt cggcggtcca
     2401 gctcagccgc cacgaggtgt gggacggcac cgatctggct gccgtcgtct accgcgacgg
     2461 cgccgtggac gtctccgtgt accagcagcc cggcagcctc gactggtccg gcctcccgcc
     2521 gggggaggtg gccacggtcg gcgaccgcgc cgtgtggttc ggggccggga accccgtggt
     2581 cgcggtcacc cagagcggag acctcgtcgt caccgtggtg tccgacgacc gcccggccgt
     2641 gctcaccgcc gtggcgggca tgccggagtg gcgacgtcgt gcgacgtggg accgggtgca
     2701 cgacgcatgc cagcgcctgg tgcgggtgtt cgcgctcgag ggctgaactc cgcgccggtc
     2761 agctccccga cgcgtacgcc tcgaggggct ctcgacgggc cttgaggatc cgccggcgct
     2821 cccgctccga cgtggcgccc cacacgccgt ggtcgatccg gttggcgagg gcgtactcca
     2881 ggcacggctc gcggaccggg cacgtggcgc agatccgctg ggcgatcgtc acgcccaccc
     2941 cgtcgctggg gaagaagacc tcgggcgggt ggtcgcggca gctgccgtcg ttcatccatg
     3001 gcgtttccat caggtgacct ccacctgttc tacgaagccg acgctcgttc ggttcccaat
     3061 tcagcacact ttagcatcaa ataaatccgg aacctcgcac cggacgcggt cgcatcgcgc
     3121 ccgggagcag cgagactggg tccatgcacc ccgaggagcc cacgagcggc gacggcatcc
     3181 cctcgacccc gccgacccgg gaatccatgg ccgacctcac cgcaccgagg gcctggcccc
     3241 gcctcgaccc cccgccgcat ccgggcgacc gcgaccgtgc cgtcggtcgg tcggcgctgg
     3301 tcggcggcgt ggtcggcggc ctcgtcgccc tgctgatcct cggtgtggcc gcgctctggc
     3361 ccaccggcgg gggaggcgag gacgtcgcca ccggcgaccg gcccccgatg acgaccgcca
     3421 cgggcggcgt cgacgtcggc gcagtgctct ccgcggtcga gccgtcggtc gtctccatcg
     3481 agatccgcag ctcgctcgcc gacggccagg ccggggcggg caccggcatc atcctcaccc
     3541 aggacggcct ggtgctcacc aacgcccacg tcatcgccgg cctcaaccag atccaggtcg
     3601 ggctcttcgg tggcgagcgg gccccggcgc gcctcgtcgg ctccttcccc gagtccgaca
     3661 tcgcgctgat ccagatcgag ggcgagtccg acctcgtccc cgcccagctg ggctcgtcgg
     3721 cgtccctgca ggtcggcgag gaggtgatcg ccatcggcaa cgcgctgaac ctgggcggtc
     3781 gcccgacggt gacccgcggg atcgtgtccg ccctcgaccg cagcctgtcg gccgagggca
     3841 tcacgctctc caacctcgtc cagaccgacg cggccatcaa cccgggcaac tccggcgggc
     3901 cgctggtgaa cgctgcgggt caggtggtcg gcgtcaacac cgccatcatc ggcgacgccc
     3961 agaacatcgg cttctccatc gagatcgaca tcgtgaagcc gctcatcgag gagatccgca
     4021 acggcggcgg cgacgtccgc ggcgaccagg ccttcctcgg ggtcaacacc accgacgtcg
     4081 ctgcggagtc cccggcgaac cgggccgagt tcgacatcac cgtggacgaa ggcgccttcg
     4141 tgcagagcat cgtcccggac agcgccgcgg acgaggccgg cctggagccc ggggacgtca
     4201 tcgtctcggt gaacggccgc gcgacgccgg actcggcctc agtcggcgcc atcgtccggg
     4261 agctgcaacc gggcgacgag atcacgatca gcgtcctgcg ggcgggcgca cccgtggaga
     4321 tgacggcgac gctgggccag cgggggtagc gctgcgccct gtggaggcgc cggtgccggg
     4381 cactacggtg gacgatcccg tagccgcgtc ccgtcccgga gtgcacatgt cagacgtcga
     4441 gatggaagaa gccccgctcc cgccggtcgc gcacgcacga ctcgtgtacc tcggcgcggt
     4501 ggcgccgcac tgggagatcc agtccgactg gggtgacgag gccatgatcg aggcgttccg
     4561 ctcccgggtc cgggcccgcc tcgtgctgct gccgccgcac gacccccagt tccgccgcaa
     4621 ccgcgagcgc gtgaaccgcg acgccgagcg cgagcgcatc gccctcgact gggacctcgg
     4681 ctactccgag gaccgctgac gtcgggcacg tgaccgtgcc ggtcgtcggt gtcgacctgg
     4741 gcgggaccaa gatcttcggc gcgctcgtcg acccggcgcg caccggcctc gaggcggtgc
     4801 tcgacgacgc caagtcgccc acgcccaccg gcggggtcga ctcggtcctg gacgccgtcg
     4861 aggccgtggt ccgtcggctc gaccccgagc cgagcgctgt gggcatcggc acgccaggtg
     4921 tggtcgagcc cggcaccggt cgcgtcctgc tcgcgccgaa cctgtccggg ttcgaccgcc
     4981 cggtcgaggt gcgccgcctg ctcgaggacc ggctcgggtg cccggtctcg gtcggcaacg
     5041 acgtgaacgt ggccgcgctc ggcgaggccc gggccggcgc cggggtcggc cacgacgacg
     5101 tcctggccgt ctggctgggc acggggctcg gtgccgggct ggtgctcgac gggcatctgc
     5161 gcgtcggccc ccacggcctc gcgggcgagc tcgggcactc ggtggtcatg cccggcggac
     5221 gccgctgcgg ctgcggcggg ctgggccacc tcgaggccta catcggccgc cgcgccctgg
     5281 aatccctggc tcgcgagctg cacgcggccg gtcgttcgac ccagctcgtg gagctcgccg
     5341 gcgacggacc catgaagtcc aaggtcttcc gagcggccta cgacgccggc gacgccgtcg
     5401 cccgggagct gctcgatgag gggatcctcc tgctcgccgt ggcggtggtg aacgtggccg
     5461 tcaccgtcga catcagtgcc gtcgtcgtcg ggggcgggct cggcgagcgc ttcgccgacc
     5521 tcgtcaccga gcagctcgcg tcggcgctgc gcacgctcgg ccacgccggc atcgagccgg
     5581 cggtgctccc cgcggcgctg ggggaccacg ccggcgcact gggtgccgcc cgcctcgccg
     5641 tcgaagcggg tagagatgca gggtgatgac gcccaccgac gacctcgcgc ccggcgtgcc
     5701 cccacgccgc cagcgcgccg cggtgccgga ggcccctgat ccctcgctga ccactgatcc
     5761 ggggcgctac ttcaaccgcg agctgtcgtg gctggacttc aacgctcggg tcctggccct
     5821 ggccgacgac ccgtccgtgc cgctcctcga gcgggccaag ttcctcgcca tctacgtgtc
     5881 gaacctcgac gagttcttcc aggtgcgggt ggcgggcctc aaggaccagg tctacgccgg
     5941 cgtcgaccag acctcccacg acggccgcac ggccggcgag cagctgctcg ccatccgcaa
     6001 cgacgtgctc gccctcggcc tcgagcagca gcggatcctg ctggaggagg tgctgcccgg
     6061 cctggccgag gagcgcatcc acctcacgag ctgggacgac ctcgacgacg acgcccgcaa
     6121 ggcgctctac gaggtgttcg aggagcacat cttcccggtg ctcacgccgc tcgccgtcga
     6181 cccgggccac ccgttcccct acatctccaa cctgtcgctc agcctcgccg tctctgtgaa
     6241 cgaccccgag accggcgaga cccgattcgc ccgggtgaag gtgccgtcca acctcgatcg
     6301 cttcgtgcgc atcccggggt cgcccaccac cttcgtcccc ctcgagcagg tgatctccgc
     6361 ccacctcgac gagctgttcc cgggcatgga gatccacgag tcagccacct tccgcgtgac
     6421 ccgcaacgcc gacctcacgc tcgaggagaa cgaggccgac gacctcctcg ccgccgtcga
     6481 ggtggagctg cgccgccgcc gcttcggccg ggccgtgcgc ctcgaggtcg aagccgccat
     6541 gagcgacgag ctgcgcgacc tgctggtgcg agagctcgac atcggtgacg acgacgtcta
     6601 cgacgtggtc ggcccgctcg acctgtccgg gctgtgggtg ctgcaccggc tcgaccgcca
     6661 cgacctcaag gacccgccgt ggcaggggat cacccaggcc cggctgctga aggaggacga
     6721 gaccccggcc gacttcttcg ccgagatgcg ccgggacgac atcctcgtcc accacccgta
     6781 ctcgagcttc cacaacaccg tggaggcctt cctcgcccag gccgccgccg acgaccgggt
     6841 cgtcgccatc aagatgacgc tgtaccgcac ctccggtgac agctcgatca tccggtcgct
     6901 ggtccgggcc gccgagtccg gcaagcaggt cgccgtcctc gtcgagctca aggcccgctt
     6961 cgacgaggct cgcaacatcg agtgggccaa ggccctcgag gaggtcggcg tccacgtcgt
     7021 ctacggcctc gtcgggctca agacccacac caagacgctg ctggtggtgc gcgaggagcg
     7081 cgacggcctc caccgctact gccacatcgg cacgggcaac tacaacccga agacggcgat
     7141 gatctacgag gacctcggcc tcttcaccac cgaccccgcc atcggcaccg acctgtccca
     7201 gctgttcaac tacctcaccg gcttcggccg ggccgtcgac tacgagaagc tcatcgtggc
     7261 gcccgaccac ctgcgcagcc gcctcgagga gctcatcgcc aacgaggcga gctacggccc
     7321 cagcggccgg atcgtggcga agatgaacgc tctgtccgat gccggcatga tcgacagcct
     7381 ctacgaggcg tcccaggccg gcgtcagcat ggacctcgtc gtgcgcggga tctgctgcct
     7441 gcgccccggg gtgcctgggc tgtcggagac gatccgcgtg cgctccatcg tgggccgata
     7501 cctcgagcac agccgcatct accagttcgc caacggcaac ggccgtggct cgccggcgtg
     7561 gttcatcggc tcgcccgacc tcatggggcg caacatcaac cggcgggtcg aggccctcgt
     7621 gccggtcga
//
LOCUS       DATISU010000069        14975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_562192, whole genome shotgun sequence.
ACCESSION   DATISU010000069 DATISU010000000
VERSION     DATISU010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 14975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14975
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562192"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VLR27_03065"
     CDS             complement(<1..251)
                     /locus_tag="VLR27_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HSP02454.1"
                     /translation="MGIVDEDIARVRAATDIVALIGEYVQLRQVGQRWRGLCPFHAEK
                     TGSFYVNAIDGLYHCFGCQASGDAITFVREQEHLGFAEAV"
     gene            311..2170
                     /locus_tag="VLR27_03070"
     CDS             311..2170
                     /locus_tag="VLR27_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02455.1"
                     /translation="MLGTMTVAGAVLAAEESGTLGGSALASWAPVNTLLIAYAVVVAL
                     LWWYGGSAAGDRFPLAVLPRIGRGLTRLTGIPGWAAIAIGQSLFALLVAGVGFYSDVS
                     WHIALGRDEQLFTAPHAGILLGLLGILSAAVFGTIVATLDGWDRGWKVAGFRVPWSML
                     PMGALGIGAVSGFPMDEVWHQAFGVDVTMWSPTHMLMIMGASFTGLAGWLVLADTGIR
                     PSHSRWAAFLHGLAAWLTLQGLVASQGEFSFGVPQFAQVFQPMLISLAAGIGLVAIRL
                     VLGPGRALLITVIYAFTQMVQLGPGDGPVETRHGATFLASAIVVEIVARLAGTADRTR
                     FAVAAGIGIGTVGLAGEWAWNTGAHQAWNTSLLPYAVATSVIAAIGGAILGAGFAGAF
                     RFAVPGRRIGTAALVVGVVLTALPVLWFLPRNTGEVEADIALEPVGTTTFGVDEIEAE
                     GAIVTVTLTPADAADDAHWFQATSWQGGGLVLEDMVEIEPGVWRSEGPVPVEGLWKTL
                     VRLHRNGSELMAAPIWFPDDPEIGEPEIPAVDRTIAMGAETQYLLRETEEGDLPWLTP
                     VVHGYLALTVLGWLLAFVVGVRRIGGAGTPAGPVPSDRTGPSEAEPVPAGSVR"
     gene            2245..3810
                     /locus_tag="VLR27_03075"
     CDS             2245..3810
                     /locus_tag="VLR27_03075"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSP02456.1"
                     /translation="MSSGPNAPAPRIPGVDLLEVIGSGGSGVVYRGRQAAFDRDVAVK
                     VARHVDDADGAPAARWEREVAAVGRLSNHPNIVPVFDAGITDDGSPYLVMPHVPKGSL
                     GDRIRREGPVPPDEVASIGARLADALATVHAAGVLHRDVKPDNVLWSPHGEPQLTDFG
                     IARLEDLTTTSDLALQATISYAAPEVLAGEPATEASDIYGLGATLYACLTGSSPHPSG
                     GGENVAALVARVLEQDPPSLAARGVPADLAAVIERAMARQPADRHVDATRFRHELERV
                     RTDPVAAVAPGPDPTQRLDTSHLAATSAGAVAEPSPARHVVAAPPPPRQDPPDEPPAD
                     NDDGRRGTNVVLWGAVAIVLLLAVGLVVLAGMRDDDGDTATTDTTEAVPETTTEPPST
                     TEATTTTAAPTTTEADPTTTTTEATATTVGESSAGDPGEVAVTYFATLDADRLDEAWQ
                     MTTSRFQAQQDRDSWEAFWGGHDIDVVGDPRVDRDTGIVIVPLTYDGQREDYRLEMVS
                     QGGAWLIDGPVGN"
     gene            complement(3826..5151)
                     /locus_tag="VLR27_03080"
     CDS             complement(3826..5151)
                     /locus_tag="VLR27_03080"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HSP02457.1"
                     /translation="MAEPTPDPELFDKVVNLCKRRGFVFPSAEIYGGFRSTYDYGPVG
                     VLLLRNVKDAWWRSMVQLRHDVVGLDASILSPPPVWEASGHLANFTDPLVDCRECKER
                     FRLDKLDDPETCPNCGAKGSFTEARQFNLMFKTHAGPVEGKGHEVFLRPETAQGMFLN
                     FKNVLETSRKKPPFGIAQVGKSFRNEITPGNFVFRTREFEQMEMEFFVPPDEADQWYE
                     YWCQTRYDWYVDHGMPTDALRLRPHDADELSHYSSGTSDVEFLFPWGWDELEGIANRG
                     DYDLTQHANHSNTKLEYFDQQANERYVPHVIEPAAGATRTAMAFLMAAYDEETVNEEQ
                     RTVLRFHPRIAPYKVAVLPLSKKDDLVGVSDEVLGLLQPHYMCDFDVTQAIGRRYRRQ
                     DEIGTPYCVTIDFDTLEDRSVTIRERDSMEQERVPIEGLRERLAERLGF"
     gene            5336..6580
                     /locus_tag="VLR27_03085"
     CDS             5336..6580
                     /locus_tag="VLR27_03085"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HSP02458.1"
                     /translation="MTAPLQYGTPPAGDPTAVMGRRSLAWIADLLIYVAIVAAGFASM
                     AEYTEIPPGVLEEEACDLFEEQNPGAADACIAADGSIYLLDTGEAGVQTVLSLGWLAF
                     FIVLQGLTGGSPGKLLFGLRVVDQYGAKCGLGKSLARTLLWVVDAAPWIIPLVGPIAA
                     FTSSGHRRVGDLAAGTYVVASSSVGQPILTSNAPIPAGQQAWGAAPPTAPPTAPPAAD
                     QGWQTAPPRDGRWESPAADAQPDLDFRTLDVPESGERVDEQAPTVHADPDWSHAPDLD
                     VTPPPAVQELDERADADAAAAGQAPPGAPEWEPPTSPGSGDPEAWEPPTSPSADWSSS
                     APHDPAPATEPPPFAAPGADGPPTAPPQSEAPEPPAAAPRYELPPPQWDQARGTYIQW
                     EPNRQAWLQWDDGNQRWKPIDS"
     gene            complement(6632..7366)
                     /gene="recO"
                     /locus_tag="VLR27_03090"
     CDS             complement(6632..7366)
                     /gene="recO"
                     /locus_tag="VLR27_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HSP02459.1"
                     /translation="MSGSRLYRDEGVVLRTYKLGEADRIVVLATEQHGKVRAVAKGVR
                     KTKSKFGARLEPLSHVALQLYEGRNLDTITQVEGIDAFRPIRDDLDRYAAAMTVLEVV
                     DQLAMERESDPHRFRMLVGVLRTIADNDNPLVVPAFELKVLAHEGLGLQVDECVSCGA
                     GDPLVAIDLLSGGMRCPTCRQGRAVSPEALRLLRAILGGRLSTVLAEEPSPAAAEVAA
                     IATEAVEQLIERRLRTPGVLGHHELT"
     gene            complement(7363..8088)
                     /gene="uppS"
                     /locus_tag="VLR27_03095"
     CDS             complement(7363..8088)
                     /gene="uppS"
                     /locus_tag="VLR27_03095"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HSP02460.1"
                     /translation="MAVEGIDPARVPRHIAAVMDGNGRWAERRGLKRTEGHAAGEEAL
                     LDATNGCLDLGVDWFTVYAFSTENWKRPTDEVRYLMGFNESLLLRRRDELHEKNVRMR
                     FIGRRGWRVPKRVLRRMDESLELTQHNTGMTLTIAFDYGGRAELVDAVRAIVAEGTPP
                     EKITEKTIARHLYDPDMPDPDLMIRTSGEQRISNYLLWQLAYSELTFPDVLWPDFRRE
                     HLFDAVRDYQERQRRFGGVERSS"
     gene            8171..9073
                     /locus_tag="VLR27_03100"
     CDS             8171..9073
                     /locus_tag="VLR27_03100"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HSP02461.1"
                     /translation="MASIKEKLASKPVIGPLLRVQDRFGEVQGDPLANGIALQIFLSL
                     IPLLLVAIAVVGFIAGGDATFTGDVIDFLGLPADGDAAGALGTTIESAEESRQAASII
                     GVLGLLWRGLAVVAAVQRAIDNVWQTKSEGFKDKARAVLWLLGAAVIFVASFAVTTVL
                     NFLPGFLAPVSILVGLAVNLGLFLWTFSELGRLPVGWRALVPGAILCAVGFEVLKLVG
                     TIYVPQLVANSSALYGSLGVVIALLAWLAFFGRLLVYGAVVNVLRWESGHGTLQVPLE
                     VPRVDTAIAVGADRSGAVVDRLDE"
     gene            9126..10982
                     /locus_tag="VLR27_03105"
     CDS             9126..10982
                     /locus_tag="VLR27_03105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HSP02462.1"
                     /translation="MAAPEIAETLEKVVAQLPGGGEVRDGQRVMAEAVADAIESGRHL
                     VVQAGTGTGKTLAYLVPAVVTGARVVVATATKALQDQLAGKDLPFLDTHVGGFSWAIL
                     KGRSNYVCRQRVAELLDDRQGRLDVANDDPGLTAVLEWANETEIGDRAELALEPRAAV
                     WDAVSVGSDECPGASKCPKGETCFAEGARRRAAEADVIVVNTHLYGTHIAAGGVVLPP
                     HDLVVLDEAHRVEEVVSSTSGSELTSGRISAAAYAVGRVIADDGLVTDILAISARIAD
                     VLDDLVGQRLDPPPARLVDQLTQLRTKLDAASTALKAVPESNVDVVARRNRASQQLLG
                     LSEAILRASVVPTGSVAWVEGRPGSAKLRIAPIDVAGLLEELLWRETTAVLCSATIPP
                     KLPTRLGLPKANTTEIDVGSPFDYEANAVLYCATHLPDPRQERADALHDEIAALIEAA
                     GGRTMALFTSWKAMQAAVEALRPRLDTPVLAQGEQPKPALLSAFTDEPAASLFATMSF
                     WEGVDVPGPSLSCVIIDKLPFARPDDPLLEARREQVGRRAFELIDLPRASTLLAQGAG
                     RLIRTADDTGVVAVLDPRLATAGYRWDLIRGLPPMRRTKDRSEVLALLGELR"
     gene            10984..11598
                     /locus_tag="VLR27_03110"
     CDS             10984..11598
                     /locus_tag="VLR27_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HSP02463.1"
                     /translation="MRSLSAVAILAGPIVLSGYLVGAVVALWLTREDEAAPRPADLVV
                     EAFAALAVSSAAWWTIEQVSPGSGFSRIGYFSNQVLSAWQSVALWSGLAVVTGICAPV
                     FRRFHGGPGLAPAGALLAVHYPWFLFATVAAGALGFGLTRSARLATGAAIAVLLPTAW
                     LGWVLEWLPAWGLPAGPEATVWAMVLTIVLGTRWWHDRPLGAAY"
     gene            complement(11592..12401)
                     /locus_tag="VLR27_03115"
     CDS             complement(11592..12401)
                     /locus_tag="VLR27_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HSP02464.1"
                     /translation="MADRIDLQEAARRLGVHYQTAYRWVREGRVPAVRVRGRYELEVS
                     DVEAFARERDAPAPATERRGRREWGRSADRLLAALCDGDERTASEVVSRLHAQGESTL
                     DVLSRVVVPAMVGIGERWSAGELTVAHEHRASEVVSRLLVGIDQRRPGRPRGTVVVTA
                     PPGEQHGLPVDMAGAVLREDGWNVELLGRDMPADALLDFVTSLAPDLVVLTVTTPANA
                     AAAESVSLQLTDAGHDVLVGEPGRTLSDLVQGARAVRAEQRRLLARRSEVQ"
     gene            complement(12452..13303)
                     /gene="era"
                     /locus_tag="VLR27_03120"
     CDS             complement(12452..13303)
                     /gene="era"
                     /locus_tag="VLR27_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HSP02465.1"
                     /translation="MRSGFVTFVGRPNVGKSTLLNQILGEKVSIVSDKVQTTRSQIRG
                     VLNLAEDAGGPAQVVFVDTPGIHKPRTPLGDRLNATASSAVRDVDVVCLLVDATMPIG
                     RGDRFIAGKVPKDAVLVVNKVDRASPEQVLAQLSAASEFGLESYFPVSARTGDGVDDL
                     VAHLVSRLPEGPQYYPEGMTSDQPDAIWVAELVREQLLAVTRDELPHSIATRVTELEW
                     PYIRVEIMVERDSQKGIVIGHKGAVLKEVGSKVRSQLREGAYLELQVVVRKDWQRRPA
                     DLDDLGF"
     gene            complement(13300..14592)
                     /locus_tag="VLR27_03125"
     CDS             complement(13300..14592)
                     /locus_tag="VLR27_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HSP02466.1"
                     /translation="MSLPLTVVFVAVLLAVVVVLALCETVLAHTSRARVAMLADEGAS
                     RARDLVDLLARRQTNLAVIVVVRHAAQVGLVILVLAHAERYSPEPLAWAAVLAGTITV
                     AAFVEGIAKTVALVRTDAVGYRVAPVGRVVTRLPLLPLLAERLGRRIRRGENGVDDDH
                     ERPSVSEEELLAFAEVAVEAEAIEEEEQALIASIIEFGDTIVREVMVPRLDMVTVEAT
                     WRVSDVMEVIILNGFSRIPVIGTGIDDIVGLAYAKDLMRSERDGTGDEPVGQHVRPAR
                     VVPEQQRVSVLLPQMQAEQFHMAVVIDEYGGVAGLVTLEDLIEELVGEIVDEYDVEDP
                     MAEPLPGGAYRVNARMPLDEVNDLLDADLPEGDWDTIGGLLFSWLGHVPTNGESVDIG
                     SHRLTASRVQGRRIGLVRIDRIEPAEESDVDAASERAR"
     gene            complement(14589..>14975)
                     /gene="ybeY"
                     /locus_tag="VLR27_03130"
     CDS             complement(14589..>14975)
                     /gene="ybeY"
                     /locus_tag="VLR27_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HSP02467.1"
                     /translation="ATTMAELNERHMGKSGPTDVLAFPIDDDGLEHGRWPDNGTSGPD
                     RPPLDMDDVPVLLGDVVVCPSVAAAQAAEHAGQPQHDGTLEDEVALLVVHGILHVLGH
                     DHAEPDETAAMQAKEQSHLAAFHRRP"
BASE COUNT         1974 a         5630 c         5241 g         2130 t
ORIGIN      
        1 accgcctcgg cgaagcccag gtgctcctgc tcgcgcacga aggtgatcgc atcgcccgac
       61 gcctggcagc cgaagcagtg gtagaggccg tcgatcgcgt tgacgtagaa cgaaccggtc
      121 ttctcggcgt ggaaggggca caggcctcgc caccgctggc cgacctggcg cagctgcacg
      181 tactcaccga tgagggcgac gatgtcggtg gccgcacgga ctcgtgcgat gtcctcgtcg
      241 acgatgccca cacgccggtt ctagtccgct cacgttgacc ctgcgacccc acatggttac
      301 cgtaggtaac atgctgggaa ccatgaccgt ggccggagcc gtgctcgccg ccgaggaatc
      361 aggcaccctc ggcggcagcg ccctggcgtc gtgggcgccg gtcaacaccc tcctgatcgc
      421 ctacgccgtg gtcgtcgccc tcctgtggtg gtacggcgga tcggcggcgg gtgaccggtt
      481 cccgctcgcc gtcctgccgc gcatcggccg gggcctgacc cgcctcaccg gcatcccggg
      541 gtgggcggcg atcgccatcg gccagtcgct gttcgcgctg ctcgtcgccg gcgtcggctt
      601 ctacagcgac gtctcctggc acatcgccct cgggcgcgac gagcagctgt tcaccgcacc
      661 gcacgccggg atcctcctcg gcctcctcgg catcctgtcg gcggcggtgt tcggcaccat
      721 cgtggccacg ctcgacggct gggaccgggg ctggaaggtg gccggcttcc gggtgccgtg
      781 gtcgatgctg ccgatgggtg ccctcgggat cggggccgtg tccggcttcc cgatggacga
      841 ggtctggcac caggccttcg gcgtcgacgt caccatgtgg agccccaccc acatgctcat
      901 gatcatgggg gccagcttca ccggcctggc cggctggctc gtgctcgccg acaccggcat
      961 ccggccgtcc cactcccgct gggccgcctt cctccacggc ctcgccgcct ggctcaccct
     1021 ccaaggtctg gtggcgagcc agggcgagtt cagcttcggc gtcccgcagt tcgcccaggt
     1081 cttccagccg atgctcatct ccctcgccgc cggcatcggc ctcgtggcca tccggctggt
     1141 gctcggcccg gggcgggcgc tgctcatcac cgtcatctac gcgttcaccc agatggtgca
     1201 gctcggcccc ggcgacggcc cggtcgagac ccgccacggc gccaccttcc tcgcctcggc
     1261 gatcgtggtc gagatcgtcg ctcgcctcgc cggcacggcc gaccgaaccc ggttcgccgt
     1321 ggcggccggc atcggcatcg gcacggtcgg cctcgccggc gagtgggcgt ggaacaccgg
     1381 tgcccaccag gcctggaaca cctcgctgct cccctacgcc gtggccacct ccgtcatcgc
     1441 cgcgatcggc ggcgccatcc tcggcgccgg gttcgccggc gcgttccgct tcgctgtccc
     1501 gggtcgacgc atcggaaccg ctgcgctggt cgtgggcgtg gtgctcaccg cgctccccgt
     1561 cctgtggttc ctgccccgca acaccggcga ggtcgaggcc gacatcgcac tcgagccggt
     1621 cggcaccacc accttcggcg tggacgagat cgaggccgag ggtgccatcg tcaccgtcac
     1681 cctcaccccc gccgacgctg cggacgacgc ccactggttc caggcgacct cctggcaggg
     1741 cggcggcctg gtgctcgagg acatggtcga gatcgagccc ggcgtctggc gctcggaggg
     1801 acccgtgccg gtggaggggc tgtggaagac cctggtccgc ctgcaccgca acggcagcga
     1861 gctgatggct gcgccgatct ggttccctga cgaccccgag atcggcgagc cggagatccc
     1921 cgccgtggat cgcacgatcg ccatgggcgc cgagacccag tacctgctgc gcgagaccga
     1981 ggagggcgac ctgccctggc tcaccccggt cgtccacggc tacctcgccc tcacggtgct
     2041 cgggtggctg ctcgccttcg tcgtcggcgt ccgccggatc ggcggggccg ggacgcccgc
     2101 cgggcccgtc ccgtcggacc ggaccggacc gtccgaggcc gagccggtgc cggccggctc
     2161 ggtccgctga gcggcccgaa cacctccgag gcctgatcgc cggtcccggc acgagacggg
     2221 acaggaacgg gtagggctgt gcccatgtcc tcgggaccca acgcgcccgc accccggatc
     2281 cccggtgtcg acctcctcga ggtcatcggc agcggggggt ccggcgtcgt ctaccggggc
     2341 cgccaggccg ccttcgaccg ggacgtcgcc gtcaaggtcg cccgccacgt cgacgacgct
     2401 gacggcgcgc cggcagcgag gtgggagcgc gaggtcgccg ccgtcgggcg cctgtccaac
     2461 caccccaaca tcgtccccgt cttcgacgcc ggcatcaccg acgacggctc gccgtacctg
     2521 gtgatgccgc acgtgcccaa gggcagcctc ggcgaccgga tccgccggga gggcccggtg
     2581 ccacctgacg aggtcgcctc gatcggcgcc cggctcgccg atgcgctggc caccgtgcac
     2641 gccgccggcg tgctgcaccg cgacgtgaag ccggacaacg tgctgtggtc cccccacggc
     2701 gagccccagc tgaccgactt cggcatcgcc cgcctcgagg atctcaccac caccagcgac
     2761 ctcgcgctcc aggccaccat ctcctacgcc gcccccgagg tgctcgccgg cgagcccgcc
     2821 accgaggcct ccgacatcta cggactcggc gccaccctct acgcctgcct gaccgggtcg
     2881 tccccccacc cgagcggtgg gggcgagaac gtggccgcgc tcgtcgcccg ggtcctggag
     2941 caggaccctc cgtcgctggc ggcccgcggt gtgcccgccg acctcgccgc cgtgatcgag
     3001 cgcgccatgg cccggcagcc ggcggaccgc cacgtcgacg ccacccggtt ccgccacgag
     3061 ctcgagcgcg tgcgcaccga ccccgtcgcc gccgtcgcgc cgggaccgga cccgacgcag
     3121 cggctcgaca ccagccacct ggcggcgacg tccgccggcg cggtcgccga accgtccccg
     3181 gctcgccacg tcgtcgcggc gccgccaccc ccgcgccagg acccgcccga cgagccaccc
     3241 gcagacaacg acgacggtcg tcgcggcacc aacgtggtgc tgtggggtgc ggtcgccatc
     3301 gtcctgctgc tggccgtggg cctcgtcgtc ctcgccggca tgcgtgacga cgacggtgac
     3361 acggcgacga cggacaccac ggaggccgtg ccggagacca ccaccgagcc cccgagcacc
     3421 accgaggcga cgaccaccac cgcagcgccc accacgaccg aagccgaccc gaccacgacg
     3481 accaccgagg cgacggccac gaccgtcggt gagtcctctg ccggcgatcc cggcgaggtc
     3541 gccgtcacgt acttcgccac cctcgacgcc gatcggctcg acgaggcgtg gcagatgacg
     3601 acgtctcgct tccaggccca gcaggaccgg gactcctggg aggcgttctg gggcggccac
     3661 gacatcgacg tggtcggcga cccccgggtc gaccgcgaca ccggcatcgt gatcgtgccc
     3721 ctgacctacg acggccagcg cgaggactac cgcctcgaga tggtcagcca gggcggggcg
     3781 tggctcatcg acggcccggt cgggaactga gccgggccgg gacggtcaga acccgaggcg
     3841 ctccgcgagc cgctcgcgca ggccctcgat cggcacccgc tcctgctcca tggagtcgcg
     3901 ctcgcggatg gtgaccgagc ggtcctcgag ggtgtcgaag tcgatcgtca cgcagtacgg
     3961 ggtgccgatc tcgtcctggc ggcggtagcg gcggccgatg gcctgggtga cgtcgaagtc
     4021 gcacatgtag tggggttgca gcaggccgag cacctcgtcg gacacgccga ccaggtcgtc
     4081 cttcttggac aggggcagca ccgcgacctt gtacggagcg atgcgcgggt ggaagcgcag
     4141 cacggtgcgc tgctcctcgt tcaccgtctc ctcgtcgtag gcggccatga ggaacgccat
     4201 ggcggtgcgg gtcgcgccgg cggcgggctc gatgacgtgg ggcacgtagc gctcgttggc
     4261 ctgctggtcg aagtactcga gcttggtgtt cgagtggttc gcgtgctgcg tcaggtcgta
     4321 gtcgccgcgg ttggcgatgc cctcgagctc gtcccagccc cacgggaaca ggaactccac
     4381 gtcggaggtg cccgagctgt agtggctcag ctcgtcggca tcgtggggac gcaggcggag
     4441 cgcgtcggtg ggcatgccgt gatcgacgta ccagtcgtag cgggtctggc accagtactc
     4501 gtaccactgg tcggcctcgt ccggcgggac gaagaactcc atctccatct gctcgaactc
     4561 gcgggtccgg aacacgaagt tgccgggcgt gatctcgttg cggaacgact tgccgacctg
     4621 ggcgatgccg aagggcggct tcttgcgcga cgtctccagc acgttcttga agttgaggaa
     4681 catgccctgc gcggtctccg ggcgcaggaa gacctcgtgg cccttgccct cgaccgggcc
     4741 ggcgtgggtc ttgaacatga ggttgaactg gcgggcctcg gtgaaggagc ccttggcgcc
     4801 gcagttcggg cacgtctcgg ggtcgtcgag cttgtcgagg cggaaccgct ccttgcactc
     4861 ccggcagtcg acgagcgggt cggtgaagtt ggccaggtgg cccgaggcct cccacaccgg
     4921 cggcggggac aggatcgacg cgtcgaggcc gaccacgtcg tggcggagct ggaccatgga
     4981 gcgccaccag gcgtccttga cgttgcggag caggagcacg cccacggggc cgtagtcgta
     5041 ggtcgagcgg aagccgccgt agatctcggc cgaggggaac acgaagcccc ggcgcttgca
     5101 caggttgacg accttgtcga acagctcggg atcgggcgtg ggttcggcca tagggggcgg
     5161 gagcctaccg atgcccctgc gaggtcctga gatccgttcg ggacgccacg cccctccgca
     5221 gcacgatcgg cggcttcgcc gccgcagggc ccgtgtcctc gggtcgcctc gcctgacggc
     5281 tcggctcctg agccgttgcc gaggggccga cggggggttc tggtagaccc gcggcgtgac
     5341 cgctcccctg cagtacggca cgcccccggc cggcgatccc acggcggtga tggggcgacg
     5401 cagcctggcc tggatcgccg acctgctgat ctacgtcgcg atcgtcgccg ccgggttcgc
     5461 gtccatggcg gagtacaccg agatcccgcc gggcgtcctc gaggaggagg cctgcgacct
     5521 gttcgaggag cagaaccccg gcgcggccga cgcctgcatc gccgccgacg gcagcatcta
     5581 cctgctcgac acgggcgaag ccggcgtcca gaccgtcctg tccctcggct ggctcgcctt
     5641 cttcatcgtc ctgcagggcc tcaccggcgg atcgcccgga aagctgctct tcggcctgcg
     5701 cgtcgtcgac cagtacggcg cgaagtgcgg cctgggcaag agcctggccc gcacgctcct
     5761 gtgggttgtg gacgcggcgc cgtggatcat cccgctggtc gggccaatcg ctgccttcac
     5821 ctcctcgggc caccgccgcg tcggcgacct cgctgccggt acctacgtcg tggcctcgtc
     5881 cagcgtcggg cagccgatcc tgacctcgaa cgcgccgatc cccgccggcc agcaggcgtg
     5941 gggcgccgcc ccgcccaccg cgcccccgac ggccccgccg gccgccgacc agggttggca
     6001 gacggcgccg ccccgcgacg ggcgctggga gtcccccgct gccgatgccc agcccgacct
     6061 ggacttccgc accctcgacg tccccgagag cggcgagcgg gtggacgagc aggcgcccac
     6121 ggtgcacgcg gaccccgact ggtcccacgc ccccgacctc gacgtcaccc cacccccggc
     6181 cgtccaggag ctcgacgagc gagccgacgc cgatgcagcc gccgccggcc aggcaccccc
     6241 cggcgcaccc gagtgggagc cgcccaccag ccccggcagc ggggaccccg aggcctggga
     6301 gccgccaacg agccccagcg ccgactggtc gtcctcggcg ccccacgacc cggccccggc
     6361 gaccgagccg ccgcccttcg ccgcccccgg cgccgatggc ccgccgaccg caccaccgca
     6421 gtccgaggcc cccgagcccc cggcagccgc ccctcgctac gagctccctc cgccccagtg
     6481 ggaccaggcc cgcggcacct acatccagtg ggagccgaac cgccaggcct ggctccagtg
     6541 ggacgacggc aaccagcgct ggaagcccat cgacagctga caccgcaggc gtcaggagga
     6601 actggcagcg gccgcggaac cgcggcgctc gtcaggtcag ctcgtggtgg ccgagcacgc
     6661 cgggcgtccg caaccggcgc tcgatgagct gctccacggc ctcggtggcg atggcggcga
     6721 cctccgcggc ggccggtgac ggctcctcgg cgagcaccgt cgagagccgt ccaccgagga
     6781 tggcgcgcag cagccgcagg gcctcgggcg acaccgcccg tccctgccgg cacgtggggc
     6841 agcgcatgcc gccgctgagc aggtcgatgg ccaccagcgg atcgccggcc ccgcacgaca
     6901 cgcactcgtc gacctggagc ccgaggccct cgtgggccag caccttcagc tcgaacgccg
     6961 gcaccaccag cgggttgtcg ttgtcggcga tcgtgcgcag gacgccgacc agcatccgga
     7021 accggtgcgg gtcggactcg cgctccatgg ccagctggtc gaccacctcg agcacggtca
     7081 tcgccgcggc gtagcggtcg aggtcgtcgc ggatcgggcg gaaggcatcg atgccctcca
     7141 cctgggtgat ggtgtcgagg ttgcggccct cgtacagctg caaggcgacg tgcgacagcg
     7201 gctcgaggcg ggcgccgaac ttcgacttgg tcttgcgcac ccccttggcg acggcccgca
     7261 ccttgccgtg ctgctcggtg gcgagcacca cgatgcggtc ggcctcgccg agcttgtagg
     7321 tgcgcaggac cacgccctcg tcccggtaga gccgggaccc gctcacgagg accgctcgac
     7381 gcccccgaag cggcgctgtc gctcctggta gtcccggacc gcatcgaaga ggtgctcgcg
     7441 ccggaagtcg ggccacagca cgtcggggaa cgtgagctcg ctgtaggcca gctgccagag
     7501 caggtagttg gagatccgct gctcgccgga cgtgcggatc atcaggtcgg gatccggcat
     7561 gtcggggtcg tacaggtggc gggcgatggt cttctcggtg atcttctccg gcggcgtgcc
     7621 ctcggcgacg atggcccgca ccgcgtcgac cagctcggcc cggccgccgt agtcgaacgc
     7681 gatcgtcagc gtcatgccgg tgttgtgctg ggtgagctcg agggactcgt ccatgcggcg
     7741 cagcacgcgc ttggggacgc gccacccccg ccggccgatg aagcgcatgc gcacgttctt
     7801 ctcgtgcagc tcgtcgcggc ggcgcagcag cagcgactcg ttgaacccca tgaggtagcg
     7861 cacctcgtcg gtgggccgct tccagttctc ggtggagaag gcgtagacgg tgaaccagtc
     7921 gaccccgagg tccaggcagc cgttggtcgc gtcgagcagg gcctcctcgc cggcggcgtg
     7981 gccctcggtg cgcttcagcc cgcgccgctc ggcccacctg ccgttgccgt ccatgacggc
     8041 ggcgatgtgg cgcggcaccc gcgccgggtc gatgccctcg acggccatgg gccgacgcta
     8101 cctctggcga cggagccgac cgcgacaggg tcatgtctgg acctgcgcca cttccgggta
     8161 acaaggggcc gtggcgtcca tcaaggagaa gctcgcgtcc aagccggtga tcggaccgct
     8221 gctgcgcgtc caggaccgct tcggcgaggt gcagggcgat ccgctcgcca acggcatcgc
     8281 tctgcagatc ttcctgtcgc tcatccccct gctgctcgtc gccatcgcgg tcgtgggctt
     8341 catcgccggc ggcgacgcga ccttcaccgg cgacgtcatc gacttcctcg gcctccctgc
     8401 cgacggcgac gccgccggcg ccctggggac caccatcgag agcgccgagg agtcccgcca
     8461 ggccgcgtcg atcatcggcg tgctcggcct gctgtggcgg ggcctcgccg tggtcgctgc
     8521 agtccagcgg gcgatcgaca acgtgtggca gaccaagtcg gaggggttca aggacaaggc
     8581 tcgtgccgtg ctctggctgc tcggagccgc cgtcatcttc gtcgcgtcgt tcgcggtgac
     8641 gaccgtgctc aacttcctcc ccggcttcct ggcgccggtc tccatcctcg tgggtctcgc
     8701 cgtgaacctc ggcctgttcc tgtggacgtt cagcgagctc ggccggctgc ccgtcggctg
     8761 gcgggcgctg gtgccggggg cgatcctctg cgcggtcggg ttcgaggtcc tcaagctggt
     8821 gggcacgatc tacgtccccc agctcgttgc caactcctcg gccctgtacg ggtcactcgg
     8881 tgtcgtcatc gccctgctcg cctggctcgc gttcttcggc cggctcctcg tgtacggcgc
     8941 cgtcgtgaac gtgctgcgct gggagtccgg ccacggcacc ctccaggtcc ccctcgaggt
     9001 gccccgtgtc gacaccgcca tcgccgtcgg cgccgatcgg tctggcgcgg tggtcgatcg
     9061 gctcgacgag tagtcgacac gtccgatcgc ccggtgtgtc accgggggcg cggtaccgtc
     9121 gcacggtggc cgcccccgag atcgccgaga cgctggagaa ggtcgtcgcc cagctccccg
     9181 gcggcggcga ggtgcgcgac ggccagcggg tcatggccga ggccgtggcc gacgccatcg
     9241 agtccggccg ccacctggtg gtgcaggccg gcaccggcac gggcaagacg ctggcctacc
     9301 tcgtcccggc cgtggtcacc ggcgcccgcg tggtggtcgc caccgccacc aaggccctgc
     9361 aggaccagct cgccggcaag gacctcccct tcctcgacac ccacgtcggc gggttctcct
     9421 gggcgatcct caagggccgg tcgaactacg tgtgccgcca gcgggtggcc gagctgctcg
     9481 acgaccgcca gggccgcctc gacgtggcca acgacgatcc cggcctcacc gccgtgctcg
     9541 agtgggccaa cgagaccgag atcggcgacc gggccgagct cgccctggag ccgcgggccg
     9601 cggtgtggga cgcggtgtcg gtcggctccg acgagtgccc cggcgcctcg aagtgcccca
     9661 agggcgagac ctgcttcgcc gagggcgccc gccgccgtgc cgccgaggcc gacgtgatcg
     9721 tggtgaacac ccacctctac ggcacccaca tcgccgccgg cggggtcgtg ctcccacccc
     9781 acgacctcgt cgtcctcgac gaggcccacc gggtcgagga ggtcgtgtcg tccacgtcgg
     9841 gcagcgagct caccagcggg cgcatcagcg cagcggccta cgccgtcgga cgggtcatcg
     9901 ccgacgacgg gctggtcacc gacatcctcg ccatctcggc ccgcatcgcc gacgtgctcg
     9961 acgatctcgt cggccagcgt ctcgacccac ccccggcccg cctcgtcgac cagctcaccc
    10021 agctccggac gaagctcgac gcggccagca cggcgctcaa ggcggtgccg gagtccaacg
    10081 tcgacgtggt ggcccgccgc aaccgcgcct cccagcagct cctcggcctg tccgaggcca
    10141 tcctgcgggc cagcgtcgtc cccaccggca gcgtcgcctg ggtggagggg cgcccgggtt
    10201 cggcgaagct gcggatcgcc ccgatcgacg tggccggcct gctcgaggag ctgctgtggc
    10261 gcgagaccac ggcggtgctc tgctcggcca cgatcccgcc gaagctgccc acccgcctcg
    10321 gcctcccgaa ggccaacacc accgagatcg acgtcggcag cccgttcgac tacgaggcca
    10381 acgcggtcct gtactgcgcc acccacctgc ccgacccccg ccaggagcgc gccgacgcct
    10441 tgcacgacga gatcgcggcg ctcatcgaag ccgcgggcgg gcgcaccatg gccctgttca
    10501 cctcgtggaa ggcgatgcag gcagcggtcg aggcgctgcg cccccggctc gacacgccgg
    10561 tgctggccca gggcgagcag cccaagccgg cgctgctgtc ggccttcacc gacgagcccg
    10621 ccgccagcct gttcgccacc atgtcgttct gggagggggt ggacgtcccc ggcccgtcgc
    10681 tgtcgtgcgt gatcatcgac aagctgccct tcgcccgccc cgacgacccg ctcctcgagg
    10741 cccggcgcga gcaggtcggc cggcgggcct tcgagctcat cgacctcccc cgcgccagca
    10801 cgctgctcgc ccagggcgcc gggcggctca tccgcaccgc cgatgacacc ggcgtcgtcg
    10861 ccgtgctcga cccccgcctc gccaccgccg gctaccgctg ggacctcatc cgcggcctgc
    10921 cgccgatgcg tcgcaccaag gaccgctccg aggtcctcgc gctgctcggg gagctccgct
    10981 gaggtgcgca gcctgtcggc cgtcgccatc ctggccggcc ccatcgtcct gtccggctac
    11041 ctggtcggcg ccgtcgtcgc cctctggctg acccgggagg acgaggccgc accccggccg
    11101 gccgatctgg tggtcgaggc cttcgccgcc ctcgccgtgt cctccgcggc gtggtggacg
    11161 atcgagcagg tctcccccgg cagcggcttc tcccgcatcg gctacttctc caaccaggtc
    11221 ctgtcggcct ggcagtccgt ggccctgtgg tccgggctcg cggtggtgac ggggatctgc
    11281 gcgccggtgt tccggcgctt ccacggcggt ccggggctgg caccggccgg cgccctcctc
    11341 gccgtgcact acccgtggtt cctcttcgcc accgtcgcgg cgggcgcgct cggcttcggc
    11401 ctcacccgct cggctcggct ggccacgggt gcggccatcg cggtcctgtt gcccaccgcc
    11461 tggctcggct gggtcctcga gtggctcccg gcctgggggc tgcccgccgg cccggaggcc
    11521 acggtctggg cgatggtgct caccatcgtg ctcggcacgc ggtggtggca cgaccgtccc
    11581 ctcggcgctg cctactgaac ctcgctgcgg cgagccagca ggcggcgctg ctcggcccgc
    11641 accgcccgcg cgccctggac cagatccgac agcgtccgcc ccggctctcc caccaggacg
    11701 tcgtgaccgg catcggtgag ctggaggctc accgactcgg cggccgcagc gttcgccggc
    11761 gtggtgaccg tcagcacgac caggtcgggg gcgagggagg tcacgaagtc gagcagggcg
    11821 tcggccggca tgtcccggcc gagcagctcg acgttccagc cgtcctcgcg gagcacagcc
    11881 ccggccatgt cgacgggcag gccgtgctgc tcgccgggcg gcgccgtgac gaccacggtc
    11941 ccgcgcggcc ggccgggacg ccgctggtcg atgccgacga gcaggcgcga caccacctcc
    12001 gatgcccggt gctcatgggc caccgtcagc tcgccggcgg accaccgctc gccgatgccg
    12061 accatggccg ggaccacgac ccgcgacagc acgtcgaggg tggactcacc ctgggcgtgc
    12121 aggcgcgaca cgacctccga ggcggtccgc tcgtcgccgt cgcacagcgc cgccagcaac
    12181 cggtcggccg agcgacccca ctcgcgccga ccacggcgct cggtcgccgg tgcgggtgcg
    12241 tcgcgctccc gggcgaaggc ctcgacgtcg ctgacctcga gctcgtagcg gccccgcacc
    12301 cgcaccgccg gcacccgccc ctcccgcacc caccggtacg cggtctggta gtgcaccccg
    12361 agccgacgag cagcctcctg gaggtcgatg cgatcggcca tggtcccggc agcgtacgtc
    12421 gccgagaacc cgggatgaac gcggcagaaa gctagaagcc gaggtcgtcc aggtcggcgg
    12481 gacggcgctg ccagtccttg cgcaccacga cctgcagctc caggtacgcg ccctcccgca
    12541 gctgggaccg caccttggat cccacctcct tcaggacggc tcccttgtgc ccgatcacga
    12601 tccccttctg ggagtcgcgc tcgaccatga tctcgacgcg gatgtagggc cactcgagct
    12661 cggtgacccg cgtcgcgatg gagtggggca gctcgtcgcg ggtgaccgcc agcagctgct
    12721 cacgcaccag ctcggccacc cagatggcgt cgggctgatc ggaggtcatg ccctccgggt
    12781 agtactgcgg cccctccggc aggcgggaga ccaggtgggc gacgaggtcg tcgacgccgt
    12841 ccccggtgcg cgccgagacc gggaagtagg actcgagacc gaactcggag gccgcggaca
    12901 gctgggccag cacctgctcc ggactggccc ggtcgacctt gttcacgacg agcaccgcgt
    12961 ccttcgggac cttcccggcg atgaagcgat cgccacgacc gatcggcatg gtggcatcga
    13021 ccagcaggca cacgacgtcg acgtcgcgca ccgcgctgct ggccgtggcg ttgagccggt
    13081 cgcccagcgg cgtcctcggc ttgtggatgc ccggggtgtc gacgaagacg acctgggccg
    13141 gcccgcctgc gtcctcggcc aggttcagca cgccccggat ctgggaccgg gtcgtctgca
    13201 ccttgtccga gacgatcgag accttctcgc cgaggatctg gttgagcagc gtggacttgc
    13261 ccacgttcgg gcgacccacg aaggtgacga agccggacct catcgggcac gctccgacgc
    13321 cgcgtcgacg tcggactcct cggccggctc gatgcgatcg atgcgcacga ggccgatccg
    13381 gcggccctgc acgcgcgacg ccgtgagccg gtgcgagccg atgtcgaccg actcgccgtt
    13441 cgtcgggacg tggccgagcc agctgaagag caggccgccg atggtgtccc agtcgccttc
    13501 ggggaggtcg gcgtcgagca ggtcgttcac ctcgtccagc ggcatgcggg cgttcacccg
    13561 gtaggccccg cccggcaggg gctcggccat cgggtcctcg acgtcgtact cgtcgacgat
    13621 ctcgccgacc agctcctcga tgaggtcctc cagcgtgacg aggccggcca ccccgccgta
    13681 ctcgtcgatg accacggcca tgtggaactg ctcggcctgc atctggggca gcagcaccga
    13741 cacccgctgc tgctcgggca cgacgcgcgc cgggcgcacg tgctggccga cgggttcgtc
    13801 gccggtgccg tcgcgctcgg agcgcatgag gtccttggcg taggcgaggc cgacgatgtc
    13861 gtcgatgccg gtgccgatga ccgggatgcg gctgaagccg ttgaggatga tgacctccat
    13921 cacgtcgctc acccgccagg tcgcctcgac cgtgaccatg tcgaggcggg gcaccatcac
    13981 ctctcggacg atcgtgtcgc cgaactcgat gatggacgcg atgagggcct gctcctcctc
    14041 ctcgatggcc tcggcctcga ccgcgacctc ggcgaaggcg agcagctcct cctccgacac
    14101 cgaggggcgc tcgtggtcgt cgtcgacgcc gttctcgccg cgccggatgc gccggcccag
    14161 ccgctcggcc agcagcggga gcagcgggag gcgggtgacg acgcggccga ccggcgcgac
    14221 ccggtacccg acggcgtcgg tgcgcacgag ggcgacggtc ttcgcgatgc cctcgacgaa
    14281 ggcggcgacc gtgatcgtcc cggcgaggac cgccgcccac gccagcggct cgggcgagta
    14341 ccgctcggca tgggccagga ccaggatcac gagcccgacc tgggcggcgt ggcggacgac
    14401 gacgatgacc gcgaggttgg tctggcgacg agccaggagg tcgacgaggt cgcgagcccg
    14461 ggaggcgccc tcgtcggcca gcatcgcgac gcgggcgcgg ctggtgtggg cgaggacggt
    14521 ctcgcacagg gcgagcacca cgaccacggc gagcaggacc gcgacgaaga cgacggtgag
    14581 gggaaggctc acgggcggcg gtggaacgcg gccaggtggg actgctcctt ggcctgcatc
    14641 gccgcggtct cgtcgggttc ggcgtggtcg tgaccgagca cgtgcaggat gccgtgcacc
    14701 acgaggagag ccacctcgtc ctcgagcgtg ccgtcgtgct ggggctggcc ggcgtgctcg
    14761 gcggcctggg cggcggcgac cgaggggcac acgacgacgt cgccgagcag caccggcacg
    14821 tcgtccatgt cgagcggggg gcggtcgggg ccgctggtgc cgttgtcggg ccagcgaccg
    14881 tgctcgaggc cgtcgtcgtc gatcgggaag gccagcacgt cggtggggcc ggacttgccc
    14941 atgtgccgct cgttgagctc ggccatggtg gtggc
//
LOCUS       DATISU010000070         1679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_143031, whole genome shotgun sequence.
ACCESSION   DATISU010000070 DATISU010000000
VERSION     DATISU010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143031"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /gene="dapA"
                     /locus_tag="VLR27_03135"
     CDS             complement(<1..732)
                     /gene="dapA"
                     /locus_tag="VLR27_03135"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HSP02468.1"
                     /translation="MGALRESRFGRVLTAMVTPFDGDGRLDLDGTQRLARWLVDHGSD
                     GLVVNGTTGESPTLSATESVDLFRAVRQAVDVPVIAGTGSNDTAHAIRQSEAAAGIGV
                     DGLLVVAPYYNKPSQAGLLRHFRMIAEATDLPIVLYDIPGRTSRKVDSDTILTLAHEV
                     PTIVGLKDAAGSPAETAGVIAHAPEDFDVYSGDDNLTLPLLSVGARGAISVASHWVGD
                     QIRAMFDAFDAGDVAEATRINQTLLP"
     gene            complement(732..1367)
                     /locus_tag="VLR27_03140"
     CDS             complement(732..1367)
                     /locus_tag="VLR27_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02469.1"
                     /translation="MSVVSPPRLLALHGLRLHGRAEPATVAEYMGADVDAIDAELRAL
                     EAQGLVDHRIGRFPGFGLTPDGRAEGERLLAEELDQHGLRRHVEAAYEEFLVCNRQLL
                     HICTSWQLRSINGTTVPNDHADADHDRAVIDAVDSLDHHVRPVLSALRDGLDRFAGHE
                     TRLRCALDHVLAGEHDWLTKPMFPSYHSCWFELHEDLLATLGRERTTEGSP"
     gene            complement(1373..>1679)
                     /locus_tag="VLR27_03145"
     CDS             complement(1373..>1679)
                     /locus_tag="VLR27_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSP02470.1"
                     /translation="GSGPMPLEHVVHRQTRQTAELYGLLDRGLLAPGMRADVNVIDHA
                     TLGAHAPEIVHDLPSGARRLLQRAHGYRHTVCAGAVTVTDDEFTGELPGRLLRGPRS"
BASE COUNT          225 a          542 c          674 g          238 t
ORIGIN      
        1 cgggagcagg gtctggttga tgcgggttgc ctcggcgacg tcgccggcgt cgaaggcgtc
       61 gaacatcgcc cggatctggt cgcccaccca gtgggaggcg acgctgatcg ccccgcgggc
      121 cccgacggag agcagcggca gggtcaggtt gtcgtcgccg gagtagacgt cgaagtcctc
      181 gggggcgtgc gcgatgacgc cggcggtctc cgccgggctc ccggcggcgt ccttgaggcc
      241 gacgatggtg ggcacctcgt gggcgagggt gaggatcgtg tcgctgtcga ccttgcggct
      301 ggtgcggccc gggatgtcgt agagcacgat cggcaggtcg gtcgcctcgg cgatcatgcg
      361 gaagtggcgg agcaggccgg cctgggacgg cttgttgtag tacggcgcca ccaccagcag
      421 gccgtcgaca ccgatgccgg cggctgcctc ggactggcgg atggcgtggg cggtgtcgtt
      481 ggagccggtg ccggcgatga ccggcacgtc gaccgcctgg cgcacggccc ggaacaggtc
      541 gacggactcg gtggccgaca gcgtgggcga ctcgcccgtg gtgccgttga cgaccaggcc
      601 gtcgctgccg tggtcgacga gccagcgagc gagccgctgg gtgccgtcga ggtcgagacg
      661 gccgtcgccg tcgaacggcg tgaccatggc ggtgaggacc cggccgaacc ggctctcgcg
      721 gagtgcgccc atcatgggct cccttcagtg gtgcgttctc gcccgagcgt cgcgagcagg
      781 tcctcgtgca gctcgaacca gcaggagtgg tagctcggga acatcggctt ggtgagccaa
      841 tcgtgctccc ccgccagcac gtggtccaac gcacatcgca gacgggtctc gtgaccggcg
      901 aagcggtcga ggccgtcgcg cagcgcggac agcaccggac gaacgtggtg gtcgagggag
      961 tcgaccgcgt cgatcaccgc tcggtcgtgg tccgcatcgg cgtggtcgtt gggcaccgtg
     1021 gtgccgttga tcgagcgcag ctgccagctg gtgcagatgt ggaggagctg gcggttgcag
     1081 accaggaact cctcgtaggc cgcctcgaca tggcgccgca ggccgtgctg gtcgagctcc
     1141 tcggcgagca ggcgctcgcc ctcggcccgc ccgtcgggcg tgagcccgaa gccggggaag
     1201 cgaccgatgc ggtggtcgac gagtccctgg gcctcgaggg cccggagctc ggcgtcgatg
     1261 gcgtcgacgt cggcacccat gtactcggcc accgtcgccg gctcggcgcg accgtgcagg
     1321 cgcaggccgt gcagggccag cagccggggc ggggagacga ccgacatcgg gcctaggacc
     1381 ggggaccccg gagcaggcgg ccgggcagct cgccggtgaa ctcgtcgtcg gtgacggtga
     1441 ccgcaccggc gcagacggtg tggcggtagc cgtgggcccg ctgcaggaga cggcgggccc
     1501 ccgagggcag gtcgtgcacg atctcgggtg cgtgggcacc gagggtggcg tggtcgatca
     1561 cgttgacgtc ggcccgcatc cccggcgcca gcaggccgcg gtcgagcagc ccgtagagct
     1621 cggcggtctg gcgcgtctgg cggtggacga cgtgctcgag gggcatcggg cccgacccc
//
LOCUS       DATISU010000071         9473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_282859, whole genome shotgun sequence.
ACCESSION   DATISU010000071 DATISU010000000
VERSION     DATISU010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9473
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282859"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VLR27_03150"
     CDS             <1..378
                     /locus_tag="VLR27_03150"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799054.1"
                     /note="catalyzes the formation of
                     2-N(omega)-(L-arginino)succinate from L-citrulline and
                     L-aspartate in arginine biosynthesis, AMP-forming; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HSP02471.1"
                     /translation="ALIKAHVDLESICLERDLQREKYRLESRYAELVYDGLWFSPLRE
                     ALDAFIVESQKHVTGEVRLHLAPNTCEVEGRRSDSSLYDYGLATYDAEDSFRHEDSAG
                     FVRLWGLGIETWSQRQGPGSRKR"
     gene            389..1792
                     /gene="argH"
                     /locus_tag="VLR27_03155"
     CDS             389..1792
                     /gene="argH"
                     /locus_tag="VLR27_03155"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HSP02472.1"
                     /translation="MSEDDGSHTLWHGRFESGPAEDLLAYTVSLSFDQQLFPDDIEGS
                     RAHVRGLGRVGILASDEVTVVLAALDQVDTELTGGTFRFLESDEDIHTAVERRVTELA
                     GSAGAKIHTGRSRNDQVATDLRLWCKRELLAVAGDVLALQQVLLDRAEAAGDAYLPGY
                     THLQRAQPVLLAHHLLAHAWALARDVDRILDARRRLDVSPLGAGALAGSSLPLDPPSV
                     AEDLGFAGVFDNSLDAVSDRDFVAESLFAVTLVAIHLSRMGEEIVLWSSDEFGFVRLD
                     DGYSTGSSMLPQKKNPDIAELARGKAGRVIGDLTGLLATLKGLPIAYNRDLQEDKEPL
                     FDAVEQVRLALRATAGQYATLTMRTERMAEAADAPTAAATDLAEFLVRAGTPFRDAHA
                     IVGALVRQSVDDGASLAELVTAHPDLGPDAAALIEPGVPVTMRTTHGGAGPAAVAAQL
                     DRFRARLSTDRTRVQEV"
     gene            1793..2176
                     /locus_tag="VLR27_03160"
     CDS             1793..2176
                     /locus_tag="VLR27_03160"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HSP02473.1"
                     /translation="MRYSLDVRYSECDMQQVVHNSVYLRWCDDLADTFFRDLAVGLES
                     GSWDIMVKAASITWSAPARLRDTVDIDIQISRFGNTSFDVTYVGTVGGEPVFEATMVY
                     VCVVPGTLETAPVPQELRDAVAGDG"
     gene            2169..2831
                     /locus_tag="VLR27_03165"
     CDS             2169..2831
                     /locus_tag="VLR27_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HSP02474.1"
                     /translation="MAEPGPLVGRGRAADVYDVGGGRVLRRYRTEHDSAPEAAIMVHL
                     AGQGFPVPAVHDVDGRDLVMDRVDGPSQLDVLPSRPWRAGDFGRELADLHRRLDAVPP
                     PPGRTGRVLHLDLHPGNVLCTPDGPVVIDWSNADVGDGADDLATTWLLLAVGRPEGGP
                     VIQALAAVLRRRLLGAFLGGVDRDAARGALGAVCDRRTLDPNMGAGEVAAIRALAETA
                     RG"
     gene            2828..3415
                     /locus_tag="VLR27_03170"
     CDS             2828..3415
                     /locus_tag="VLR27_03170"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HSP02475.1"
                     /translation="MKPVERSVLARDPREVAPELLGKVLAHDTPEGRRSGRIVEVEAY
                     CGPIDAGAHTYRGRTARNATMFGPAGLLYVYFTYGMHWCVNVVCGDEDEGVAVLVRAL
                     APLEGIEAMREARGPAAKRDRDLCSGPAKAAQALGLHGVHDGVDLLGGGPVGLWDDGT
                     PPPPDPVQATRIGLSAGAEHPWRWYVAGDPNVSRR"
     gene            3434..4042
                     /locus_tag="VLR27_03175"
     CDS             3434..4042
                     /locus_tag="VLR27_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSP02476.1"
                     /translation="MPAADLRLLAADAPELRASERLADARAAVAAADVDPGAEGHRRT
                     ILDFIDAHDDALHRSCRTGHLTGSALVVDADGVRVVVLHHRKLDRWLQPGGHCDGDAN
                     LAAVALREATEETGIDGLRVLPVAVDLDVHEVAPPDDGPHLHLDVRYLVHAPPGAEPA
                     GNHESNDIRWASLDDLAGLDLDEGTWRMVRHGLARHQELVRR"
     gene            4039..5016
                     /gene="rsgA"
                     /locus_tag="VLR27_03180"
     CDS             4039..5016
                     /gene="rsgA"
                     /locus_tag="VLR27_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HSP02477.1"
                     /translation="MSTGPGLEALGWDESRRAEVEAIDAPGGVPGRVVRQDRGWVQVA
                     RADGVHGARTRADRVGTPVVGDWVVVVDDEVAAVLERRGVLRRADPVGEGEQVLAANL
                     DRVLVVAGLDRPVKAGRIQRAAAQAWDAGAEPTVVLTKADLVADPESLRAGVAAEHPG
                     TDVVAVSSRTGQGVDELAHALGGDTVVLLGESGAGKSSLLNALAGAEVALTGDVRAFD
                     AKGRHTTTRRELHLLPGGGIVVDTPGIRAIGLFVDPEAIDAAFSDIDDLSAQCRFNDC
                     THDHEPGCAVRAAVEAGELSAERLEAFGELHAEAEWARRPEHERRKRRP"
     gene            complement(5017..5574)
                     /locus_tag="VLR27_03185"
     CDS             complement(5017..5574)
                     /locus_tag="VLR27_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02478.1"
                     /translation="MPDSPATEPSDRRMGLRRLGVIAVVVSFVGIWGYVMYLTFFEGR
                     AEPRDRMADTRFTAAAEETCAESGDFFEQLPFANEVNSPAERADLLDEATDELEVMVT
                     RLEGLVPPRDADEAVAVERWLDDYRTFIQDRREYAEAQRDPSSDRYDQPFAVTDRGGF
                     QIDVLIDDFALINDMDSCETPDDVG"
     gene            5665..6945
                     /gene="tyrS"
                     /locus_tag="VLR27_03190"
     CDS             5665..6945
                     /gene="tyrS"
                     /locus_tag="VLR27_03190"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HSP02479.1"
                     /translation="MSSLLDDLRARGLVQDSTDLDALADRLAEGPIALYYGCDPSAPS
                     LHAGNLIGLLVLRRFADAGHRPVALVGGATGMIGDPGGRSEERNLLDEETLAANVAGI
                     GAQVQRIVGEVATVVNNRDWTAGIGVLEFLRDVGKHVTVNQMLAKESVKARVSSEHGI
                     SYTEFSYQLLQANDFWHLHAHQGVELQIGGSDQWGNLVAGVDLIRRRSQASAHAFTWP
                     LITRADGAKFGKSTGGAIWLDAAQTSPYQFFQYWMNVDDRDVQRFLLQLTLLEVDEVR
                     DLVAVHTDAPQERAAQRRLAHEVTSLVHGTDAALAAAEASSVLFGGDPTDASAAAWAV
                     VAEEVDTVGAPEGASAEEGIDPVPVLVGSGLAKSNSDARRLVRDGGVRITGRRIEDGD
                     RIGPGDVRHGHYVLLARGRKAFAVLDLEKDPLSS"
     gene            7409..8957
                     /locus_tag="VLR27_03195"
     rRNA            7409..8957
                     /locus_tag="VLR27_03195"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            9392..>9473
                     /locus_tag="VLR27_03200"
     rRNA            9392..>9473
                     /locus_tag="VLR27_03200"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1472 a         3286 c         3323 g         1392 t
ORIGIN      
        1 gccctcatca aggcccacgt cgacctcgag tcgatctgcc tcgagcgcga cctccagcgc
       61 gagaagtacc gcctcgagtc ccgctacgcc gagctggtct acgacggcct gtggttctcg
      121 ccgctgcgcg aggcgctcga cgccttcatc gtggagtccc agaagcacgt gaccggcgag
      181 gtgcgcctgc acctggcgcc caacacctgc gaggtcgagg gccggcgctc ggactcgagc
      241 ctgtacgact acgggctggc cacctacgac gccgaggact cgttccgcca cgaggactcc
      301 gccgggttcg tgcgcctgtg gggcctcggc atcgagacct ggtcccagcg ccagggaccc
      361 ggctcccgga agcgctgagc cggcgacgat gagcgaggac gacggcagcc acacgctctg
      421 gcacgggcgg ttcgagtccg ggccggcgga ggacctgctc gcctacacgg tgagcctgtc
      481 cttcgaccag cagctgttcc ccgatgacat cgagggcagc cgggcccacg tgcgcggcct
      541 cggccgcgtc gggatcctcg cctccgacga ggtcaccgtc gtgctggccg ccctcgacca
      601 ggtcgacacc gagctcaccg gcggcacgtt ccggttcctg gagtccgacg aggacatcca
      661 caccgcggtg gagcggcggg tcaccgagct cgccggctcc gcgggcgcca agatccacac
      721 cggccgcagc cgcaacgacc aggtggccac cgacctgcgc ctgtggtgca agcgcgagct
      781 gctggccgtg gccggcgacg tgctcgccct ccagcaggtg ctcctcgacc gggccgaggc
      841 ggccggcgac gcctacctgc cgggctacac gcacctgcag cgagcccagc cggtgctgct
      901 ggcccaccac ctcctcgccc acgcctgggc cctcgcccgc gacgtcgacc ggatcctgga
      961 cgcccgtcgt cgcctcgacg tgtcgcctct cggcgccggt gccctggccg gttcgtcgct
     1021 gcccctggac ccgccgtcgg tggccgagga cctcggcttc gccggcgtgt tcgacaacag
     1081 cctcgatgcg gtcagcgacc gcgacttcgt ggccgagtcc ctcttcgccg tgaccctggt
     1141 cgccatccac ctctcccgga tgggtgagga gatcgtgctg tggagcagcg acgagttcgg
     1201 cttcgtccgc ctcgacgacg gctactccac cggctcctcg atgctcccgc agaagaagaa
     1261 ccccgacatc gccgagctcg cacgcgggaa ggccggccgg gtcatcggcg acctcaccgg
     1321 cctgctggcc acgctgaagg ggctgccgat cgcgtacaac cgcgacctgc aggaggacaa
     1381 ggagccgctg ttcgacgcgg tcgagcaggt gcgcctcgcc ctgcgggcca cggccgggca
     1441 gtacgccacg ctgacgatgc ggaccgagcg catggccgag gccgccgacg cccccactgc
     1501 ggcagccacc gacctggccg agttcctcgt ccgggccggc accccgttcc gggacgccca
     1561 tgccatcgtc ggcgcgctgg tgcgccagtc ggtcgacgac ggcgcctccc tggccgagct
     1621 ggtcaccgcc cacccggacc tcggtcccga tgccgccgcc ctcatcgagc ccggcgtgcc
     1681 cgtcaccatg cgcaccaccc acggcggtgc cggcccggcg gccgtggccg cgcagctcga
     1741 tcggttccgt gcccgcctgt ccaccgaccg cacccgcgtg caggaggtct gagtgcgcta
     1801 ctccctcgac gtccgctact ccgagtgcga catgcagcag gtcgtgcaca acagcgtgta
     1861 cctgcgctgg tgcgacgacc tggccgacac cttcttccgc gacctcgccg tcggcctcga
     1921 gagcggcagc tgggacatca tggtcaaggc cgcgtccatc acctggtcgg cgccggcccg
     1981 gctgcgggac accgtcgaca tcgacatcca gatctcgcgc ttcggcaaca cgtccttcga
     2041 cgtcacctac gtcggcaccg tcggcggcga gccggtcttc gaggcgacga tggtctacgt
     2101 ctgcgtcgtc cccgggacgc tcgagacggc gcccgtgccc caggagctgc gcgacgccgt
     2161 ggccggcgat ggctgaaccc ggaccgctcg tcggccgtgg tcgggcggcc gacgtgtacg
     2221 acgtcggcgg cggtcgcgtc ctgcggcgct accggaccga gcacgactcc gcgcccgagg
     2281 ccgccatcat ggtccacctc gccggacagg ggttccccgt gccggccgtc cacgacgtgg
     2341 acggccggga cctggtgatg gatcgcgtcg acgggccgtc gcagctcgac gtgctcccga
     2401 gccgcccgtg gcgggctggg gacttcggga gggagctggc cgacctccac cgccgcctcg
     2461 acgcggtgcc gccgcccccc gggcgcaccg ggcgggtcct gcacctcgac ctgcaccccg
     2521 gcaacgtcct gtgcacgccg gacggtcccg tcgtcatcga ctggtccaac gccgacgtgg
     2581 gcgacggcgc cgacgatctg gccaccacct ggctcctcct cgccgtcgga cggcccgagg
     2641 gcggtccggt catccaggcc ctggccgcgg tcctgcgtcg ccgcctgctc ggcgccttcc
     2701 tcggcggcgt ggaccgggat gcagcccgag gagcgctcgg ggcggtgtgc gaccggcgca
     2761 ccctggaccc gaacatgggt gccggtgagg tcgccgccat ccgcgccctc gccgagaccg
     2821 cacgcggatg aagccggtcg agcgctccgt gctcgctcgc gacccgcggg aggtcgcccc
     2881 ggagctgctc ggcaaggtcc tggcccacga cacgccggag gggcgacgca gcggtcgcat
     2941 cgtcgaggtg gaggcctact gcggtccgat cgacgccggc gcccacacct accggggccg
     3001 caccgcccgc aacgccacca tgttcggacc ggccggcctg ctgtacgtgt acttcaccta
     3061 cggcatgcac tggtgcgtga acgtggtctg cggcgacgag gacgaaggag tcgccgtgct
     3121 cgtccgggcg ctggcgccgc tcgagggcat cgaggccatg cgcgaggccc ggggaccggc
     3181 ggccaagcgg gatcgggacc tgtgcagcgg cccggcgaag gcggcccagg cgctcggcct
     3241 gcacggggtc cacgacggcg tcgacctcct cggaggcggc cccgtgggcc tgtgggacga
     3301 cggcacgccg ccgccgcccg atcccgtgca ggcgacccgc atcggcctct ccgcgggcgc
     3361 tgagcacccg tggcgctggt acgtggccgg tgaccccaac gtctcgcgcc gctgaccggc
     3421 ctagcctccc gtcgtgcccg ccgccgatct ccgtctgctg gccgccgacg cccccgagct
     3481 gcgcgcctcg gagcggctgg ccgacgcccg ggccgcggtg gccgccgccg acgtggatcc
     3541 gggagccgag gggcaccgcc ggaccatcct cgacttcatc gatgcccacg acgacgccct
     3601 gcaccgatcg tgccgcaccg gccacctgac cggttccgcg ctggtggtcg acgccgacgg
     3661 cgttcgggtc gtcgtgctcc accaccgcaa gctcgaccgc tggctgcagc ccggaggtca
     3721 ctgtgacggc gacgccaacc tcgccgccgt cgccctgcgg gaggccaccg aggagacggg
     3781 catcgacggg ctccgggtgc tgcccgtcgc cgtggacctc gacgtgcacg aggtcgcccc
     3841 gcccgacgac ggcccccacc tgcacctcga cgtccggtac ctcgtgcacg cacccccggg
     3901 ggcggagccg gcggggaacc acgagtccaa cgacatcagg tgggcctcac tcgacgacct
     3961 cgccgggctc gatctggacg agggcacctg gcgcatggtc cgccacggcc tcgcccgcca
     4021 ccaggagctg gtgcgccgtt gagcaccggg ccggggctgg aggcgctggg ctgggacgag
     4081 tcccgccgag cggaggtcga ggcgatcgac gcgccgggcg gggtgcccgg tcgcgtggtg
     4141 cgtcaggacc ggggctgggt ccaggtcgcc cgagccgacg gcgtccacgg tgcccgcacc
     4201 cgagcggacc gcgtcggcac acccgtggtc ggcgactggg tcgtggtcgt cgacgacgag
     4261 gtcgcggcgg tgctcgagcg ccgtggcgtg ctccgccggg ccgatccggt gggggagggg
     4321 gagcaggtgc tcgccgccaa cctcgaccgg gtgctggtgg tggccggcct ggaccggccc
     4381 gtcaaggccg ggaggatcca gcgggccgcg gcccaggcct gggacgccgg cgccgagccc
     4441 accgtcgtgc tgaccaaggc cgacctcgtc gccgaccccg agtcgctgcg ggccggggtc
     4501 gccgccgagc accccgggac cgatgtggtc gccgtgtcct cccgcaccgg gcagggtgtc
     4561 gacgagctcg cccacgccct cgggggcgac acggtcgtgc tgctggggga gtccggcgcc
     4621 ggcaagtcca gcctcctcaa cgcgctggcc ggggccgagg tcgccctcac gggagatgtc
     4681 cgcgcgttcg acgccaaggg ccggcacacc accacgcgcc gcgagctcca cctgctgccc
     4741 ggtggcggca tcgtcgtcga cacgcccggc atccgcgcca tcgggctctt cgtcgacccg
     4801 gaggccatcg acgccgcgtt ctccgacatc gacgacctct ccgcgcagtg ccggttcaac
     4861 gactgcacgc acgaccacga gccgggctgc gccgtccgcg ccgcggtcga ggccggtgag
     4921 ctctcggccg agcggttgga agccttcggc gagctgcacg ccgaggcgga gtgggcccgc
     4981 cgcccggagc acgagcggcg caagcgtcgg ccctagtcag ccgacgtcat ccggggtctc
     5041 gcagctgtcc atgtcgttga tgagggcgaa gtcgtcgatg agcacgtcga tctggaagcc
     5101 gccccggtcg gtcacggcga agggctggtc gtagcggtcg ctgctcgggt cccgctgcgc
     5161 ctcggcgtac tcgcgccggt cctggatgaa cgtccggtag tcgtcgagcc agcgctccac
     5221 cgccacggcc tcgtcggcgt cgcgcggcgg caccagcccc tcgagccgcg tgaccatcac
     5281 ctcgagctcg tcggtggcct cgtcgagcag gtcggcccgc tcggcagggg agttgacctc
     5341 gttggcgaac gggagctgct cgaagaagtc accggactcg gcgcaggtct cctcggccgc
     5401 cgcggtgaag cgggtgtcgg ccatgcggtc gcggggttcg gcccgcccct cgaagaacgt
     5461 gaggtacatg acgtagcccc agatgccgac gaagctgacc acgacggcga tcaccccgag
     5521 gcgtcgcaga cccatgcggc ggtcggaggg ttcggtggcg ggggagtcgg gcacgaccac
     5581 gatggtcgtc cctcgctgcc ccggcacgcc agacggggaa cgcgccgtcg ggccgacgcg
     5641 gcgcgccgcg tactgtcggc gcccatgagc agcctcctcg acgacctccg ggcccggggc
     5701 ctcgtgcagg acagcacgga cctcgacgcc ctggccgacc gcctcgccga gggaccgatc
     5761 gccctgtact acgggtgcga cccgtcggcg ccgagcctgc acgccggcaa cctgatcggc
     5821 ctgctggtgc tgcggcgctt cgccgatgcc ggccaccggc ccgtcgcgct cgtcgggggt
     5881 gccaccggca tgatcggcga cccgggcggg cgatccgagg agcggaacct gctcgacgag
     5941 gagacgttgg cggccaatgt ggccggcatc ggcgcgcagg tccagcgcat cgtcggtgag
     6001 gtcgcgacgg tcgtgaacaa ccgggactgg acggcgggca tcggcgtgct ggagttcctg
     6061 cgcgacgtgg gcaagcacgt caccgtcaac cagatgctgg ccaaggagtc ggtgaaggcc
     6121 cgggtcagct ccgagcacgg catctcctac accgagttca gctaccagct cctccaggcc
     6181 aacgacttct ggcacctcca cgcccaccag ggggtcgagc tgcagatcgg cggatcagac
     6241 cagtggggca acctcgtggc cggcgtggac ctgatccggc gccgctccca ggcgagcgcc
     6301 cacgccttca cctggcccct gatcacccgg gccgacggcg ccaagttcgg caagtccacc
     6361 ggcggtgcga tctggctcga cgcggcgcag acctcgccct accagttctt ccagtactgg
     6421 atgaacgtcg acgaccggga cgtgcagcgc ttcctgctgc agctgaccct gctcgaggtg
     6481 gacgaggtcc gcgacctcgt cgccgtgcac accgacgcgc cccaggagcg ggcggcccag
     6541 cgtcgcctgg cccacgaggt gacctcgctg gtgcacggga ccgatgcggc cctggccgca
     6601 gccgaggcgt ccagcgtgct gttcggcggc gaccccaccg atgcctccgc ggcggcgtgg
     6661 gccgtcgtgg ccgaggaggt cgacacggtc ggcgcgcccg agggcgcatc cgcggaggag
     6721 gggatcgacc cggtgccggt cctggtgggc tcggggctgg ccaagtccaa ctccgacgcc
     6781 cgtcgcctcg tgcgcgacgg cggggtccgg atcacgggcc ggcggatcga ggacggggac
     6841 cggatcggcc ccggcgacgt gcgccacggg cactacgtcc tgctcgcgcg aggacgaaag
     6901 gcctttgccg tcctggatct ggaaaaagat cccctgtcga gttgacgctc ggggcgcgca
     6961 tcggtaactt gatctctcgc cccgcacagc cgctacggcc ggaacggggc acctggcaga
     7021 gtcagtgccg gcaccgatgc cgcagacgac gtccagcgca atggcttcct cggaagcgcg
     7081 ccgggcggga tttgctccaa catcgagtac ctcgctacct tgcccactcc ggcaacaccg
     7141 gaaggacttg ctcctccgat tgccaatgag ctccttgaaa acagaagaga ggaatgacgt
     7201 gcgagtgcgg gcgatcgcct ccgcttcggt ggatgtgatc gctatgtaat ttgcacctgt
     7261 catctttgag ttcgaaatgg gtctacccaa gacccaggac acaagaacat gtgccagcag
     7321 gtggaggcca gtcaggccga agccggctct caatgaatga tcgagtcgac gaccagaggt
     7381 cgcaagaccg acggtcgtcg caatgatctt gacggagagt ttgatcctgg ctcaggacga
     7441 acgctggcgg cacgcttaac acatgcaagt cgaacgaggt ccatccgctc gcaagagtgg
     7501 tgaagaccta gtggcgaacg ggtgagtaac acgtgagaaa cctgccccga agaccgggat
     7561 aactcaccga aaggtgagct aataccggat accctcacga cgcttcatgg cgatgtgagg
     7621 aaatggattc cgcttcggga gggtctcgcg gcctatcagc ttgttggtga ggtaacggct
     7681 caccaaggcg acgacgggta gctgttctga gaggaagatc agccacactg ggactgagac
     7741 acggcccaga ctcctacggg aggcagcagt ggggaatctt gcgcaatggg cggaagcctg
     7801 acgcagcaac gccgcgtgtg ggaagacggc tttcgggttg taaaccactt tcagggggga
     7861 cgaagcttcc gaggttaata gcctacgggg tgacggtacc cccagaagaa gccccggcta
     7921 actacgtgcc agcagccgcg gtaacacgta gggggcaagc gttgtccgga tttattgggc
     7981 gtaaagagct cgtaggcggc tcggtaagtc gggtgtgaaa actcagggct taaccctgag
     8041 acgccactcg atactgctgt ggctagagtc cggtagggga gcgtggaatt cctggtgtag
     8101 cggtgaaatg cgcagatatc aggaagaaca ccagcggcga aggcggcgct ctgggccgga
     8161 actgacgctg aggagcgaaa gcgtgggtag caaacaggat tagataccct ggtagtccac
     8221 gccgtaaacg ttgggtacta ggtgtggggt cttatcaacg gattccgtgc cgaagctaac
     8281 gcattaagtg ccccgcctgg ggagtacggc cgcaaggcta aaactcaaag gaattgacgg
     8341 gggcccgcac aagcggcgga gcatgtggct taattcgacg caacgcgaag aaccttacca
     8401 aggcttgaca tgcagagaaa tccggcagag atgtcgggtc cgcaagggct ctgcacaggt
     8461 ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc
     8521 aaccctcgtc ctatgttgcc agcacgtgat ggtggggact cataggagac tgccggggtc
     8581 aactcggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgtct tgggctgcac
     8641 acatgctaca atggccggta caaagggctg ctaaaccgcg aggtggagcg aatcccaaaa
     8701 agccggtctc agttcggatt ggggtctgca actcgacccc atgaagtcgg agttgctagt
     8761 aatcgcagat cagcaacgct gcggtgaata cgttcccggg ccttgtacac accgcccgtc
     8821 acgtcacgaa agtcggtaac acccgaagcc agtggcccaa ctcgcaagag agggagctgt
     8881 cgaaggtggg atcggcgatt gggacgaagt cgtaacaagg tagccgtacc ggaaggtgcg
     8941 gctggatcac ctcctttcta aggagcacct ggcctgcagc cttggctgtc gggtccagac
     9001 ccacgctcga gacgttcgct ctcggggtgg agctcaaggg tggaacactg accagtaggc
     9061 cgtctcttct cgagacactc ctagtacagc cggcttgccg gtgggaacgg ggtggatcgg
     9121 gtccggggcg gcccaggcgc gctgttgggt cctgagggaa cggactcgct cttcggagcg
     9181 gatcgtttct tcggtggctg agagcctctg ctgctgctga tgctggtgcc ttcgggtgct
     9241 ggcggaggtg ggagtggggg ctctgtcacg gacagggcca gccggcaccg aaccatcggg
     9301 gcgcgtcatc gacgctgctc gggtaggcgg tgggttccgg ttggatccgc ccgtagcttg
     9361 agaactgcac agtggacgcg agcatctttg tggtcaagtt agtaagggcg cacggtggat
     9421 gccttggcac taggagccga tgaaggacgt aggaggctgc gataagcctc ggg
//
LOCUS       DATISU010000072         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_794909, whole genome shotgun sequence.
ACCESSION   DATISU010000072 DATISU010000000
VERSION     DATISU010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794909"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2090)
                     /gene="nifJ"
                     /locus_tag="VLR27_03205"
     CDS             complement(<1..>2090)
                     /gene="nifJ"
                     /locus_tag="VLR27_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HSP02480.1"
                     /translation="SSKELTPSMVGTVFDELAAPRPRRHFTVGIYDDVSHLSLPMDGT
                     LRYDRPDREVQAVFVGLGSDGTVGANKSSVKIIGEGTDLYAQGYFVYDSKKSGSVTVS
                     HLRFGPEPIRSTYLVDDADFVACHQFGLLEKVKVLDYARPGATLLVNSPFGPDDVWDN
                     LPREVQTQIVAKGIDLWVIDADAVARDTGMGNRINTIMQPCFFRLSGVLPPEEAIARI
                     KGTVEKAYGKRGRSVVERNFAAIDAALDHLCQVDVPDAATSTRTMEHTVPADAPEFVQ
                     RVTSLLMQGEGDLLPVSALPVDGVFPTATAQYEKRSIAKQIPIWDPDVCIDCGKCAIV
                     CPHATIRMKVFDTDAVADAPDDFLHKAFRSKDLADHRLTIQVAPDDCTGCGVCVDVCP
                     AKSKTDVSHKAINMEPAMEHRDLERERWDFFQTIPHLDRDLLPHDSVKGSQVLEPLFE
                     FSGACAGCGETPYIKLVTQLFGDRMIVANATGCSSIYGGNLPTTPWTVNGDGRGPAWN
                     NSLFEDNAEFGLGMRLALDVQREQAQTSLQRLAPHLGADLVARLLDNPQETEAEITAA
                     RTDVDQLRQRLASLVEQPDTAVDASHLASLVGNLVRQGVWLMGGDGWAYDIGFGGLDH
                     VLSTGRNVNILVLDTEVYSNTGGQASKATPRAAVAKFAAAGKGIGKKDLGAIARAYGN
                     VYVAQISMGANELQ"
BASE COUNT          294 a          650 c          805 g          341 t
ORIGIN      
        1 ctgcagctcg ttggcgccca tggagatctg ggccacgtac acgttgccgt aggcccgggc
       61 gatggcgccg aggtccttct tgccgatgcc cttgccggcg gcggcgaact tggcgaccgc
      121 ggcccggggc gtggccttgg atgcctggcc gccggtgttg gagtacacct cggtgtcgag
      181 gacgaggatg ttcacgttgc gtcccgtcga cagcacgtgg tcgaggccgc cgaagccgat
      241 gtcgtaggcc cacccgtcgc cgcccatcag ccacacgccc tggcggacga ggttgccgac
      301 gagggacgcc aggtgggagg cgtcgaccgc ggtgtcgggc tgctcgacga gcgatgccag
      361 ccgctggcgc agctggtcca cgtcggtgcg ggcggccgtg atctcggcct cggtctcctg
      421 ggggttgtcc agcaggcggg ccaccaggtc ggcgccgagg tggggggcga ggcgctgcag
      481 cgatgtctgg gcctgctcgc gctggacgtc gagggcgagg cgcatgccca ggccgaactc
      541 ggcgttgtcc tcgaacaggg agttgttcca ggccgggccg cgaccgtcgc cgttcaccgt
      601 ccacggggtg gtgggcaggt tgccgccgta gatcgacgag cagccggtgg cgttggccac
      661 gatcatgcgg tcgccgaaca gctgggtcac cagcttgatg tagggcgtct cgccgcaccc
      721 ggcgcaggca ccggagaact cgaacagcgg ctccagcacc tgggacccct tgacgctgtc
      781 gtgggggagg aggtcgcggt cgaggtgggg gatggtctgg aagaagtccc agcgctcccg
      841 ctcgaggtcg cggtgctcca tggccggttc catgttgatg gccttgtgcg acacgtcggt
      901 cttggacttg gccgggcaga cgtcgacgca gaccccgcag ccggtgcagt cgtcgggggc
      961 gacctggatg gtgaggcggt ggtcggccag gtccttggag cggaacgcct tgtggaggaa
     1021 gtcgtcgggc gcatcggcca cggcgtcggt gtcgaacacc ttcatgcgga tggtggcgtg
     1081 cgggcagacg atggcgcact tgccgcagtc gatgcagacg tcgggatccc agatggggat
     1141 ctgcttggcg atcgagcgct tctcgtactg cgcagtcgcg gtcgggaaca cgccgtcgac
     1201 gggcagggcg ctcacgggga ggaggtcgcc ctcgccctgc atcagcaacg acgtgacccg
     1261 ctggacgaac tcgggggcgt cggcggggac cgtgtgctcc atcgtccggg tgctcgtggc
     1321 ggcatcgggc acgtccacct ggcacaggtg gtccagggcg gcgtcgatgg cggcgaagtt
     1381 gcgctccacg accgagcgcc cccgcttgcc gtaggccttc tcgaccgtgc ccttgatgcg
     1441 ggcgatggcc tcctcgggcg gcagcacccc ggacagccgg aagaagcagg gctgcatgat
     1501 cgtgttgatg cggttgccca tgccggtgtc gcgggcgacg gcatcggcgt cgatcaccca
     1561 gaggtcgatc cccttggcga cgatctgggt ctgcacctcg cggggcaggt tgtcccacac
     1621 gtcgtcgggt ccgaacgggc tgttcaccag cagcgtggcg ccgggccggg cgtagtcgag
     1681 caccttgacc ttctcgagca ggccgaactg gtggcaggcc acgaagtcgg cgtcgtcgac
     1741 caggtaggtg gaccggatcg gctcgggtcc gaagcggagg tgcgagacgg tgaccgaccc
     1801 ggacttcttg gagtcgtaga cgaagtagcc ctgggcgtag aggtcggtgc cctcgccgat
     1861 gatcttcacc gaggacttgt tggcgcccac cgtgccgtcg gagccgaggc cgacgaacac
     1921 ggcctgcacc tcgcggtccg ggcggtcgta gcgcagggtg ccgtccatgg gcaggctcag
     1981 gtgcgacacg tcgtcgtaga tgccgacggt gaagtggcgc cggggccggg gtgcggccag
     2041 ctcgtcgaag accgtgccga ccatggacgg cgtgagctcc ttggaggaca
//
LOCUS       DATISU010000073         6120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_3528, whole genome shotgun sequence.
ACCESSION   DATISU010000073 DATISU010000000
VERSION     DATISU010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3528"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VLR27_03210"
     CDS             complement(<1..465)
                     /locus_tag="VLR27_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02481.1"
                     /translation="MMLLAAEGSGTLGGSALASWAPINTLLIAYAIVVALLWWRGEPA
                     PGDKLPLGALQRIGRGLTRTTGIPGWAAIAIGQSLFALLVAGVGFYSDVAWHIALGRD
                     NQLFTAPHAAILLGLVGILSAAVFGTLVATLDGWDRGWPVAGLRVPWSMLPLA"
     gene            546..1625
                     /locus_tag="VLR27_03215"
     CDS             546..1625
                     /locus_tag="VLR27_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HSP02482.1"
                     /translation="MLSLLAAAESDSANFVSFSTPGWVWAAFAALVVVLLLVDLVVVH
                     KDAHVVTTKEAAIESAVWISIGLLFGLVLLFWQGGDAAGEYYAGYLIEKSLSIDNVFV
                     WALVMSYFAVPQQFQFRVLFWGIFGALVLRAVFIFGGVAILERFGWVIYVFGAFLLFT
                     AIRLLKPTHDDVHPEDNFILRMVNKVVPSTTEYDGQKLFTMKNGKRVATPLFAVLVVV
                     ESSDVIFAVDSIPAILAVSREEFIVFSSNAFAILGLRALYFLLADLRNRFRYLQQGLA
                     IVLAFVGVKMLISEVYHLPTYLSLGVIAVVLTVAITLSIRNPLPEGEEPDVSTHGLPG
                     RRTPHVENASSSTGASGAPGGPAGD"
     gene            1635..3521
                     /gene="dnaG"
                     /locus_tag="VLR27_03220"
     CDS             1635..3521
                     /gene="dnaG"
                     /locus_tag="VLR27_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HSP02483.1"
                     /translation="MGIVDEDIARVRGATDIVALIGEYVQLRQVGQRWRGLCPVHTEK
                     TGSFYVNAVDGLYHCFGCQASGDAITFVREQEHLGFAEAVERLAAKVGISLNYDKPGE
                     SKTRAERKRLAEVVAAAEDWYHERLLSAPDAAAARKYLRDRGFDGDEVREWKIGWAPD
                     DWDQLARALRLSRQDAEGTGLGRVNSRGRINDFFRGRVLFPIHDADGTPLGFGGRIMP
                     GAEGSKYVNTSQTRLYDKSRVLFALDRAKIGIVAADEVVVCEGYTDVTAFFRVGIPRA
                     VATCGTALTDDHVQLLRRFARRIVLAFDADTAGQAAADRFYQWESKFDMDVVVAAMPP
                     GVDPADLAQRAPEELVAAVEGARPFLEFRLGRILDGADLDSAEHRARAAEEALGVIAE
                     HPNDLVRDQYLMRVADRCRLDVDRLRQRVASGDVRRPAPPEPPRRRRDQADAPPRDDD
                     PGPRPPGHDPDDTGPREPTRGRGGQHRPGDDPSRRPGGSRDDSPAVEVLRLAVNRPED
                     VVDWLDECLFDEALHLETYRVLVSSATIHEAIESAPPEVASFLSRLSVEEPVDDALDP
                     VLLQVRATGRRLLAVERSASDPDLEVLATLARLVDRVGPGDDGLHAARELLTFASHTG
                     GTHE"
     gene            3514..4767
                     /gene="rpoD"
                     /locus_tag="VLR27_03225"
     CDS             3514..4767
                     /gene="rpoD"
                     /locus_tag="VLR27_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HSP02484.1"
                     /translation="MSDVVEDPAVADEALAALVDAARPAGELHLDAALVELRVELTAD
                     LLTRLKAACASAGVRLDEDVHLDDDVVGAGEPVEEALSPADEVARARRRRVASRNLKA
                     VKPAAGGSSDSVRLYLREIGRVPLLNAAEEVSIAKRIEAGAIAASHLADLAASDGLDS
                     LPFAERRALQRAAKDGDRATDELIQANLRLVVSIAKRYVGRGMQLLDLIQEGNLGLMR
                     AVQKFDYTKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVESINKVHRIQRTMLM
                     ELEREPTVEELAERADLTPDRVREIMQISMDPLSLDSPVGEEDDSNLADFIGDVNADA
                     PAEAATKTLLNQAVLEALEELNEREQAVMRLRFGLEDGQARTLEQVGQEFGVTRERIR
                     QIESKTLAKLRHPHRSQKLRDYLES"
     gene            4821..4893
                     /locus_tag="VLR27_03230"
     tRNA            4821..4893
                     /locus_tag="VLR27_03230"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4854..4856,aa:Asn,seq:gtt)
     gene            5037..5369
                     /locus_tag="VLR27_03235"
     CDS             5037..5369
                     /locus_tag="VLR27_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02485.1"
                     /translation="MHIEVPHHCGRTHRGHQSLAECTWPDAAYVVGDGAYAVLARCDL
                     FTVALYETSAEARRRKRGLDANGCGKTCEGLHELEVLVEEPEVDLRRLVSAGVRSGRR
                     RRPSSPAR"
     gene            complement(5302..5511)
                     /locus_tag="VLR27_03240"
     CDS             complement(5302..5511)
                     /locus_tag="VLR27_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02486.1"
                     /translation="MSTTSRVTDEPIVEIDLTDLDDLLERRHARRFDEASVVAWLRTS
                     GGTLTERAMKAAAADLIERQRTPTA"
     gene            5816..>6120
                     /locus_tag="VLR27_03245"
     CDS             5816..>6120
                     /locus_tag="VLR27_03245"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HSP02487.1"
                     /translation="MTSAAPTLTTHSSRVAATPAAERAVGRYQLLAGLQVADVATTWV
                     ILANFSTRAEGNPVVAQMIHHGGLQLAMIGLLVVKLAVVYVLYEKQTGINIMSAIYGL
                     "
BASE COUNT          898 a         2258 c         2089 g          875 t
ORIGIN      
        1 ggcgaggggc agcatcgacc acggcacccg caggccggcg acgggccagc cgcggtccca
       61 gccgtcgagc gtggcgacga gggtgccgaa gaccgccgcc gagaggatgc ccacgaggcc
      121 gaggaggatg gcggcgtggg gtgcggtgaa gagctggttg tcgcgaccga gggcgatgtg
      181 ccaggccacg tcgctgtaga agccgacgcc ggcgacgagc agggcgaaca gcgactggcc
      241 gatggcgatt gccgcccagc cggggatgcc ggtggtccgg gtgaggcccc gtccgatgcg
      301 ctggagggcg cccagcggga gcttgtctcc gggggcgggc tcgccccgcc accacagcag
      361 cgcgacgacg atcgcgtagg cgatgagcaa cgtgttgatc ggggcccagg aggcgagggc
      421 gctgcccccg agcgtcccgg agccttcggc ggcgaggagc atcatgttac caacggtaac
      481 ataatcgggc attccggaca aggctccaac cgactcaacg ctccggcgat ggggtatgac
      541 ggcgcatgct ctcgctgctc gccgctgcgg aatcggactc cgcgaacttc gtgtcgttct
      601 ccaccccggg ctgggtgtgg gccgccttcg ccgccctcgt ggtcgtgctg ctgctcgtcg
      661 acctcgttgt cgtccacaag gacgcccacg tggtgaccac caaggaggcg gcgatcgagt
      721 cggcggtctg gatctcgatc ggcctcctgt tcggcctcgt gctcctgttc tggcagggcg
      781 gcgacgcggc cggggagtac tacgccggct acctgatcga gaagtcgctg agcatcgaca
      841 acgtcttcgt ctgggcgctg gtcatgagct acttcgccgt cccccagcag ttccagttcc
      901 gggtgctgtt ctggggcatc ttcggcgccc tggtgctgcg ggccgtcttc atcttcggcg
      961 gcgtcgccat cctcgagcgc ttcggctggg tgatctacgt cttcggcgcc ttcctgctct
     1021 tcaccgccat ccgcctgctc aagccgaccc acgacgacgt ccatccggag gacaacttca
     1081 tcctgcggat ggtgaacaag gtcgtgccgt cgaccaccga gtacgacggc cagaagctgt
     1141 tcacgatgaa gaacggcaag cgcgtggcca ccccgctgtt cgccgtgctg gtcgtggtcg
     1201 agtcgtccga tgtcatcttc gccgtggact cgatccccgc catcctcgcc gtcagccgcg
     1261 aggagttcat cgtgttctcc tccaacgcct tcgccatcct cggcctgcga gccctctact
     1321 tcctccttgc cgacctccgg aaccggttcc ggtacctgca gcagggcctg gcgatcgtgc
     1381 tcgccttcgt cggcgtcaag atgctgatca gcgaggtgta ccacctgccc acctacctgt
     1441 cgctgggcgt catcgccgtg gtcctcacgg tcgccatcac gctgtcgatc cgcaacccgc
     1501 tccccgaggg cgaggagccc gacgtctcca cccacggcct ccccggacgt cgcaccccgc
     1561 acgtcgagaa cgcctcgtcg agcacgggtg catccggtgc gccagggggt cccgccggcg
     1621 actagaaccg gcacgtgggc atcgtcgacg aggacatcgc gagggtccgc ggcgccaccg
     1681 acatcgtcgc cctgatcggc gagtacgtgc agctccgcca ggtcggccag cgctggcgcg
     1741 gcctgtgccc ggtccacacc gagaagaccg ggtcgttcta cgtcaacgcc gtcgacggcc
     1801 tctaccactg cttcggctgc caggcctccg gcgacgccat caccttcgtg cgtgagcagg
     1861 agcacctcgg cttcgccgaa gcggtcgagc ggctggccgc caaggtcggc atcagcctca
     1921 actacgacaa gccgggggag agcaagaccc gagccgagcg caagcgcctc gccgaggtcg
     1981 tcgccgcggc cgaggactgg taccacgagc gtctcctgtc cgcccccgac gccgccgcag
     2041 cccggaagta cctgcgcgac cgcgggttcg acggcgacga ggtccgggag tggaagatcg
     2101 gctgggcacc cgacgactgg gaccagctcg cccgggcgct gcggctgtcc cgccaggacg
     2161 cggagggcac cggcctcggc cgggtgaact cccggggtcg gatcaacgac ttcttccggg
     2221 ggcgggtcct gttcccgatc cacgacgccg acggcacgcc cctcggcttc ggcggccgga
     2281 tcatgcccgg cgccgagggc tccaagtacg tcaacacgtc ccagacccgg ctctacgaca
     2341 agtcccgggt cctgttcgcc ctcgaccggg ccaagatcgg catcgtcgcc gccgacgagg
     2401 tcgtcgtgtg cgagggctac accgacgtga cggcgttctt ccgggtgggg atcccccggg
     2461 ccgtggccac gtgcggcacc gcgctcaccg acgaccacgt ccagctgctg cgccgcttcg
     2521 cccgacggat cgtgctcgcc ttcgacgccg acacggccgg ccaggccgcc gccgaccgct
     2581 tctaccagtg ggagtccaag ttcgacatgg acgtggtggt ggcggcgatg ccgcccggcg
     2641 tcgaccccgc cgacctcgcc cagcgcgccc cggaggagct ggtcgccgcc gtggagggtg
     2701 ctcgcccgtt cctcgagttc cgcctcggtc ggatcctcga cggcgccgac ctggactcgg
     2761 ccgagcaccg ggcccgcgcc gccgaggagg ccctcggcgt catcgcggag caccccaacg
     2821 acctcgtgcg cgaccagtac ctcatgcggg tggccgaccg ctgccgcctc gacgtcgacc
     2881 ggctccgcca gcgggtggcg tccggggacg tgcgccggcc ggcgccgccc gagccgcccc
     2941 gccgccggcg ggaccaggcc gacgccccac cccgggacga cgaccccggc cctcgtccac
     3001 ccggccacga cccggacgac accggaccac gggagccgac gcgcgggcga ggcgggcagc
     3061 accgacccgg cgacgatccc agccgccgtc ccgggggctc ccgcgacgac tccccggcgg
     3121 tcgaggtcct gcgcctggcc gtcaaccggc ccgaggacgt cgtcgactgg ctcgacgagt
     3181 gcctgttcga cgaggccctc cacctcgaga cgtaccgggt gctcgtctcc tcggcgacca
     3241 tccacgaggc catcgagtcc gccccgccgg aggtcgcgtc cttcctgtcc cggctctccg
     3301 tggaggaacc ggtcgacgac gccctcgacc ccgtgctgct ccaggtccgg gccaccgggc
     3361 ggcggctgct cgccgtcgag cgatcggcca gcgaccccga cctcgaggtc ctcgccaccc
     3421 tggcccgcct ggtcgaccgg gtcgggccgg gagatgacgg gctccacgct gcgagggagt
     3481 tgctaacgtt cgcatcgcac acggggggca cccatgagtg atgtggtcga ggatccagcg
     3541 gtagcagacg aggcgctcgc ggcgctcgtc gacgcggccc gccccgccgg cgagctccac
     3601 ctcgacgctg ccctcgtcga gctccgggtc gagctcaccg ccgacctgct cacgcgcctc
     3661 aaggcggcgt gcgcgagcgc gggcgtccgc ctcgacgagg acgtgcacct cgacgacgac
     3721 gtcgtggggg cgggcgagcc cgtcgaggag gccctctcac cggcggacga ggtcgcgcgc
     3781 gcccgtcggc gtcgggtggc gtcgcgcaac ctcaaggcgg tcaagccggc ggccggcggc
     3841 agcagtgact cggtgcggct ctacctccgg gagatcggcc gcgtcccgct gctgaacgcc
     3901 gccgaggagg tctcgatcgc caagcgcatc gaggccggcg ccatcgccgc cagccacctc
     3961 gccgacctcg ccgcgtccga tggcctcgat tcgctgccct tcgccgagcg ccgggcgctg
     4021 cagcgggccg ccaaggacgg cgaccgcgcc accgacgagc tgatccaggc caacctgcgc
     4081 ctcgtggtgt ccatcgccaa gcgctacgtc ggacggggca tgcagctgct cgacctgatc
     4141 caggagggca acctcggcct catgcgggcc gtgcagaagt tcgactacac caagggcttc
     4201 aagttctcca cctacgccac gtggtggatc cgccaggcca tcacccgggc catcgccgac
     4261 caggcccgca ccatccgcat ccccgtgcac atggtcgagt cgatcaacaa ggtgcaccgc
     4321 atccagcgca ccatgctgat ggagctcgag cgcgagccca cggtcgagga gctcgccgag
     4381 cgtgccgacc tgacgcccga tcgcgtgcgg gagatcatgc agatctcgat ggacccgctg
     4441 tcgctggact ccccggtggg ggaggaggac gactccaacc tggccgactt catcggcgac
     4501 gtgaacgccg acgcgccggc ggaggccgcc accaagacgc tgctgaacca ggccgtcctc
     4561 gaggccctcg aggagctcaa cgagcgcgag caggccgtca tgcggctgcg gttcgggctg
     4621 gaggacggcc aggcccgcac cctcgagcag gtgggccagg agttcggcgt cacccgcgag
     4681 cggatccgcc agatcgagtc caagaccctg gccaagctgc gccaccccca ccggtcccag
     4741 aagctgcgcg actacctcga gtcctgacgg ccaccaggtt ctccggcccg gggagcagtt
     4801 gctatcgtcg gcgcttccct tccggggtag ctcagctggc agagcacgcg actgttaatc
     4861 gtgttgtcgt gggttcgatc cccacccccg gagcgcagaa cctgcaggtc ggagccggtc
     4921 ggatcgaccg gctcgcttgc tgtccgggcc cacctcgccg acatcggcag gggttcgggg
     4981 catccacggc gaagcatcgg cctcgctccg tgacgacgga gcaggggagg tcggacatgc
     5041 acatcgaagt gccccaccac tgcggccgga cccatcgcgg ccaccagtcg ctggccgagt
     5101 gcacgtggcc cgacgcggcc tacgtcgtgg gggacggcgc ctacgccgtc ctcgcccggt
     5161 gcgacctgtt cacggtcgcg ctctacgaga cgagcgcaga ggcccgccgc cgcaagcgcg
     5221 gcctggacgc caacgggtgc ggcaagacct gcgagggcct ccacgagctc gaggtgctcg
     5281 tggaggaacc cgaggtcgac ctcaggcggt tggtgtccgc tggcgttcga tcaggtcggc
     5341 ggcggcggcc ttcatcgccc gctcggtgag ggtgcctccc gaggtgcgga gccacgcgac
     5401 cacgctggcc tcgtcgaagc gccgggcgtg acggcgctcg aggaggtcgt cgaggtcggt
     5461 gaggtcgatc tcgacgatgg gctcatcggt cactcgcgac gtggtggaca tggtcgacgc
     5521 ctcctggctc gggtgcccca cctgggcgga gccataccca accgcgacgc cggcagaacc
     5581 gaaccttccg cctgattcgg acacaacctg cgctcgtgga acggcccgca ccggatcgac
     5641 agcccgccgc ataggtcgtt cggcctacgc ggtgcgcacg aagcgtggga tgcctcgcac
     5701 ggaccgaggc gacggggccg gtgtcgacgg cccggagcac gacggacgtc cggatcgtcc
     5761 ggagttcggc ggttcctgac cggtgaggac ccgggcacgt cggtagggag ggtgcatgac
     5821 cagcgcagcc cccaccctca ccacgcactc cagccgtgtc gcagccacac ccgccgccga
     5881 acgggccgtc ggccggtacc agctcctcgc cggcctccag gtggccgacg tcgccaccac
     5941 ctgggtcatc ctcgccaact tctcgaccag ggccgagggc aacccggtcg tggcccagat
     6001 gatccaccac ggcggcctcc agctcgccat gatcggcctg ctcgtggtga agctcgccgt
     6061 cgtgtacgtg ctctacgaga agcagaccgg catcaacatc atgtcggcga tctacggcct
//
LOCUS       DATISU010000074         1943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_469166, whole genome shotgun sequence.
ACCESSION   DATISU010000074 DATISU010000000
VERSION     DATISU010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469166"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VLR27_03250"
     CDS             <1..649
                     /locus_tag="VLR27_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HSP02488.1"
                     /translation="RIVRGRDDRVERIVEQADTTPEEAEIDEINTGIFCFRRSLLSPA
                     LRRIRPDNAQGELYLTDVVAVLAEAGHRVTTLVADDADETHGINDRVQLAAAEAELRQ
                     RINHGWMRAGVTFVDADQTYVDATVELGRDVTLYPGTMLRGVCTIGEGADVGPGAHLT
                     DTAVGDRARVFQTVADRARIGDDAVVGPWAALGPGDEVPSGQVTGPHYAGPGAPR"
     gene            659..1639
                     /locus_tag="VLR27_03255"
     CDS             659..1639
                     /locus_tag="VLR27_03255"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HSP02489.1"
                     /translation="MELVSKKKLHLLTGRTHLPLAEEIAALLSEPLGEANLAEFANGE
                     IHAKLGRNVRGADVFIIQSHSSVEGLTINDSIMEQLIMIDAARRASAKRIVAVCPFYG
                     YARQDRKAEGREPITAKLVADLFKAAGADRLVSIDLHSGQIQGFFDGPVDHLQARPVL
                     IDAMRSLGEDLVVVSPDAGRVKVAEQYANELHADLAIVHKRRVKGAKNAVEARDVVGE
                     VEGRCCVLIDDMIDTAGTMVAAAEQLVAHGASEVHAACTHPVLSGPAVDRIKNSSIGR
                     LICTNTLPLPPEKQFDKLEVHSIAPILAKAIDAVFEDTSVSELFGGKNQT"
BASE COUNT          319 a          746 c          618 g          260 t
ORIGIN      
        1 gcgcatcgtg cgcggccgag acgaccgggt cgagcgcatc gtcgagcagg ccgacaccac
       61 ccccgaggaa gccgagatcg acgagatcaa caccggcatc ttctgcttcc ggcgcagcct
      121 gctgtccccg gcgctgcgcc gcatccgacc ggacaacgcg caaggcgagc tgtacctcac
      181 cgacgtcgtc gccgtgctcg ccgaggccgg ccatcgggtc accaccctcg tcgccgacga
      241 cgccgacgag acccacggca tcaacgaccg ggtccagctc gccgccgccg aggccgagct
      301 gcgccagcgc atcaaccacg gctggatgcg tgccggcgtc acgttcgtcg acgccgacca
      361 gacctacgtc gacgccaccg tcgagctcgg gcgcgacgtc accctctacc cggggacgat
      421 gctgcgcggc gtctgcacga tcggcgaggg ggccgacgtc ggacccggcg cccacctcac
      481 cgacaccgcc gtcggcgacc gtgcccgggt gttccagacc gtcgccgacc gggcccgcat
      541 cggcgacgac gccgtggtcg gaccctgggc tgccctcggg ccgggcgacg aggtcccttc
      601 gggccaggtg accggccctc actatgctgg ccccggcgcg ccgaggtagg gagtcccaat
      661 ggagctcgtg tccaagaaga agttgcacct cctcacggga cggacgcacc tgccgctcgc
      721 cgaggagatc gccgcgctgc tgagcgagcc gctcggtgag gccaacctgg cggagttcgc
      781 caacggggag atccacgcca agctgggtcg caacgtgcgc ggcgccgacg tcttcatcat
      841 ccagagccac agctcggtcg agggcctcac gatcaacgac tcgatcatgg agcagctcat
      901 catgatcgac gccgctcgcc gggcctcggc gaagcggatc gtcgccgtct gccccttcta
      961 cggctatgcc cgccaggacc ggaaggccga gggccgcgaa ccgatcacgg ccaagctcgt
     1021 ggccgacctc ttcaaggccg ccggtgccga tcgcctcgtc tccatcgacc tgcactccgg
     1081 ccagatccag ggcttcttcg acggcccggt cgaccacctc caggcccgcc ccgtgctcat
     1141 cgacgcgatg cggagcctcg gcgaggacct cgtcgtcgtc tcgcccgatg ccggccgcgt
     1201 gaaggtcgcc gagcagtacg cgaacgagct ccacgccgac ctggccatcg tccacaagcg
     1261 ccgggtgaag ggcgccaaga acgccgtcga ggcccgtgac gtggtcggcg aggtcgaggg
     1321 ccgctgctgc gtgctcatcg acgacatgat cgacaccgcc ggcaccatgg tcgccgccgc
     1381 cgagcagctg gtcgcccacg gtgcgtccga ggtccacgcc gcctgcaccc acccggtcct
     1441 gtccggcccg gccgtggatc gcatcaagaa ctcgagcatc ggtcgcctga tctgcacgaa
     1501 caccctcccg ctcccgccgg agaagcagtt cgacaagctc gaggtccact cgatcgcccc
     1561 gatcctggcc aaggccatcg acgccgtctt cgaggacacc agcgtcagcg agctcttcgg
     1621 cggcaagaac cagacctagg aaactggccg tcggcaggtc acgcgggtgc atgtgtgacc
     1681 tggtgactgc cagtttccag agtcgcgaca cttgcggcgg tcggacaggc gccagttgga
     1741 gcccatccgg cgccagccgt acactgaccc gttcgtctcc acccagctag caggaccgca
     1801 tccccatggc agcagaccag atcatcctca cggccgagac cgggcgcccc accggctccc
     1861 gctcctcgcg ccgcctccgc gccgagggca agatcccggc ggtcgtctac ggcctcgaca
     1921 ccgagcccaa ggccatcgcc gtg
//
LOCUS       DATISU010000075        11094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_748493, whole genome shotgun sequence.
ACCESSION   DATISU010000075 DATISU010000000
VERSION     DATISU010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 11094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11094
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748493"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VLR27_03260"
     CDS             <1..817
                     /locus_tag="VLR27_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP02490.1"
                     /translation="DRGNGDPVVFLHAFPFNAAMWEYQYAALEADHRVIGIDLPGFGS
                     SPAPTDPKATTMDHWADLVAGVLDELGLDQPPIVAASMGGYLVFAIVRRHLDKVGPLV
                     LVATRPKSDDVDTWEKRTSQQQALEGGEDLATLAKGMVDNLLSQASLERPELVDYVLA
                     LMQHNLPEGWVAALEAMKNRPDAMGALRGMPRKTLVVAGELDRITPLAESTLITRLVA
                     DGELAVIKDSSHLPNLENPVEFNEVLKRFLDSLAEPEAEAEGADAEAEAPAAE"
     gene            complement(818..2464)
                     /gene="pgi"
                     /locus_tag="VLR27_03265"
     CDS             complement(818..2464)
                     /gene="pgi"
                     /locus_tag="VLR27_03265"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HSP02491.1"
                     /translation="MADTPPRSSLTSTSAWQALASHQPRMAELHLREIFADDPGRGDR
                     MVVQAGDLHIDFAKHRATTETLDLLVRLAEERDVAGRFHALFTGERVNTTEDRPALHT
                     ALRRRRDDRLEVDGVDVVAQVHEVLDRLSSFASRVRSGEWTGSTGRPIRHVVNIGIGG
                     SDLGPAMAATALRHWVREDLDLRFVSNVDGDDLAWALRHVDPETTLFIVASKSFTTLE
                     TLTNARHAKAWLHERLGPETRVERHFVALSTNADAVSAFGIDTANMFGFWDWVGGRYS
                     LDSAIGLSLMIGIGPAAFGELLEGFRRIDDHVRETPLADNASVLMALLNLWYRNFLGA
                     STQAVLPYSDVLARFPAYLQQLEMESNGKRVTLDGDPVDHHTGSIVWGEPGTNGQHAF
                     YQLLHQGTSLVPCDLIGFAEPTDDSLVSEHHDLLTANLLAQAEALAFGRTAEEVAASG
                     VAPSLVPHRTFPGNVPSTTILAPRLTPSVLGQLVALYEHKVAAQGFLWGINSFDQWGV
                     ELGKQLATTIASEIADGTDGDHDSSTIALLRRYRAMRGEL"
     gene            complement(2475..4316)
                     /locus_tag="VLR27_03270"
     CDS             complement(2475..4316)
                     /locus_tag="VLR27_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP02492.1"
                     /translation="MSEVMTDTDTDRDTDGAIAVLRRGIAATPELKVGIAFTVGMAVS
                     SGAGRLAVPVLIQQILDRGVLGDDGFQPGFVYPACIVAVVGILALFALARATYLRLIR
                     TAEDALYALRVRTFDHIHRLAIADHTESRRGELVARVTSDVETLAQFTQWGAIAWIVN
                     SSVIFGTLLVMLVYSWQLTVLVLIAFGPLLPLMRALQRRQLAAYDEVRDTVGSTMAEV
                     SEAVTGSAVVRAYGLEDRTRRRLRSAMTGQYRAHLRAGLFFALLFPLADVFGSVAMAG
                     VAVVGSLEGAALGITPGELVAFLFLVNLLLMPVSELSEVFDQTQTAIAGWRKILGVLD
                     MPVDVDDPEPGAELPDGPLGIEIRGVSFAYREGPQVLHHVDVRIPAGTNVAVVGETGS
                     GKSTLAKLLCRLADPDEGAIRIGGVELTTVGGGARRRHIRMVPQDGFLFDDTLAANVR
                     MGGDGQVEAATDDDVLRAFDSLGLGEWVRSLPAGIHTEVGERGGNLSVGERQLVALGR
                     AQLAEPGVLILDEATSAIDPETEQALAGALAHLSQGRTVVSIAHRLSTAELADLVLVF
                     DRGRLVEQGHHDELLALGGTYAAMHRSWMGATRDAVPGADEAGVPQP"
     gene            complement(4313..6157)
                     /locus_tag="VLR27_03275"
     CDS             complement(4313..6157)
                     /locus_tag="VLR27_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP02493.1"
                     /translation="MPTVEDKAETTATQHDTTPGVMRRGVSLLVEILRTNPWPAAGAI
                     AGAVVFATASVAGAIILGRVTDDLILPAVEGRPEVGAALVGGGVIVGMAVVRSAGVVA
                     RRYFGIMTARRMQMQWFNRLGDHYVEQPLPFFFGQPTGKLLAHADNDAERSAFAMQPL
                     PMSIGAVVMLVFGTINLALLDPLLLAIGLGLFPSLIVMNRLYTTRVVGPSARAQAHVG
                     DVSSVAHESFEGALVVKLLGLEARESTRMRTAAGDLRRERLVIGRLRAAFEPAIEALP
                     NLGTVALLAVGAWRVSEDVITTGDLVQAMVLFAILAFPMRVIGFLLEELPRSVVSRDR
                     IVDVLDIDHRVTGAPLAPEPDGRRRLPPGPLPLSVRDLSFGYGDEQVLDAISFDIEPS
                     GVVAIVGATGSGKSTLCNLLAGLARPSTGEIALGDVPLRLADPMDLRSSVALVFQETF
                     LFADTVLENLTLGEPVGDQRLQWAIDIAQAGYVDDLAHGRDTIVGERGVTLSGGQRQR
                     LALARALLRHPRLLLLDDATSAIDPVIEQRILDGLREDLDTTTLVVAHRVATIRLADR
                     VLFLDGGRLVGDGTHDELLARDPQYAALVRAYEQAGDAVVDDLPGAQP"
     gene            complement(6218..7249)
                     /locus_tag="VLR27_03280"
     CDS             complement(6218..7249)
                     /locus_tag="VLR27_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSP02494.1"
                     /translation="MTDWAIAGAGWISAIHGLAIRSLPGSRVVAVASRTEAAAQARVA
                     ETGGRAASYEELPAGADIVVVATPPTRHAVDALRAMAAGAAVLVEKPLCATLDEADRL
                     VRVVQAGGLLTYAENLLFADATGTALSEVAGLGPLRHLSARALQPRPTWGDFTRRHWG
                     GGVLFDLGVHPLALVLRAARTTPVAVSARFDASADVEVEDHAEVSLRFDSGLVAEIEV
                     SWRSPTGVWDLQAASDRGAVRFELLPEVALERNGEPVALPPLPRREPDDPAPPQLDRY
                     GYVEQLRDTEASWRAGRPSMVSDVHLGRDLLEIVCAASESARTGDEVALPWNGPRDRP
                     PIELWRPSP"
     gene            7343..9577
                     /locus_tag="VLR27_03285"
     CDS             7343..9577
                     /locus_tag="VLR27_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HSP02495.1"
                     /translation="MTRALNSLVRIAMLLALASVVVTAVGMAAAPQLTGLTEAAHGEA
                     SDVELRGLSQRSYVYAVDGSLIATLQEEENRAPITLDEVPDHVINAVLAVEDSGFWDH
                     RGVNVRATVRALLSNVDAGGTTQGGSTITQQLIKQSVLTSEQTFDRKTREVFLAVRLE
                     EQMSKEEILERYLNTIYLGNHSYGIQTAAETYFNVSASELDPGQAALLAGLIRDPIDY
                     NPFRYPEVAVERRRIALSRMVAEGHITEDDATWYNAVPLPTEPSQYTPPPDDYFVEEV
                     KQALLADPRLGETATERYDAVFRGGLQIHTTQNIGLQYLSEVARNDTLAPFALEGEPA
                     LFTAGRNLAGFDAVGTVASATVEPTTGAVRALVGGPGFGDQYKFNLATQGYRQPGSSF
                     KTFVLLELLEQGYAPQDTISGRGPCRFNLPTEEEPYEVENFNNSRGFVGSIRAMTTAS
                     SNCGYVRLGQIAGIDNVTELAGEMGVVTRNAANEIVPLDPSFFSTPLGTQEVTPLGMA
                     AAYAAIANDGHYHTPYYVERVLDRDGELIFQHSDPGKRVVSVETAQIAADILKENVES
                     GTGTAARVRGHDVAGKTGTAQDSSNGWFVGFSRQLSTAVWMGAMEGNVPMTRVGGRSV
                     TGGSYPAQIFGRLYNAVLAQMEPEPFAEPPTRRGTGTLRLDRNIDLDGYTTEPTRRRR
                     TTTRTPSRTPSRSAPATTAAPAPTTTVAPAPPPDPGNGNGNGGGGGGTSDTTPSGDFE
                     EMDG"
     gene            9574..10287
                     /locus_tag="VLR27_03290"
     CDS             9574..10287
                     /locus_tag="VLR27_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HSP02496.1"
                     /translation="MNPWKQLLDVQTLDTSLTQLDHRELQLPARTELTEVEGQLAALR
                     AEVAAVEGDKHVLDKEQKRIEDEIASIEDKIASADRQLYSGSSDVKELQALQDEIAAL
                     KRRISALEDQELELMEQVEPIDARLAGFAARQAELDEQAVALTTAIAEAEADIDRERN
                     EVRSRRDEVVAEIDPTALQEYETVRGRLGGVAVARLEHGSCGACHMKLSAVENDRILH
                     LSPDEPVRCEDCGRFLVRD"
     gene            10298..10798
                     /locus_tag="VLR27_03295"
     CDS             10298..10798
                     /locus_tag="VLR27_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02497.1"
                     /translation="MLIWYVATALAGVWAVFDSPALDYRFVMVGAVLPVGEVVLGQPG
                     PLHSLVTAVAVLALVMVATQGRRLLRRRLLGIPIGLLAHLVFSGSWADTDAFWWPLTG
                     LGLSDAEPPELARGGLSVLLELAGIALGLWWVRRVGLDDRAARDEFARTGRLPRATAP
                     PVQGTC"
     gene            10792..>11094
                     /locus_tag="VLR27_03300"
     CDS             10792..>11094
                     /locus_tag="VLR27_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSP02498.1"
                     /translation="MLTLVRHGRTEANRSGLLLGRIDAELDPLGRAQADAVAAHVGPA
                     DRVVCSPLARTRATAAAWDAPVEVDDRWIELDYGELDGTPMRDVDADLWRRWRRDVA"
BASE COUNT         1618 a         3901 c         4157 g         1418 t
ORIGIN      
        1 cgaccgtggc aacggtgacc cggtggtgtt cctccacgcc ttcccgttca acgcggcgat
       61 gtgggagtac cagtacgccg cgctcgaggc cgaccaccga gtcatcggca tcgacctgcc
      121 cggcttcggc agcagcccgg caccgaccga ccccaaggcc accaccatgg accactgggc
      181 cgatctggtg gccggcgtgc tcgacgagct cggcctggac cagccgccga tcgtcgccgc
      241 ctcgatgggc ggctacctgg tgttcgccat cgtccggcgc cacctcgaca aggtcggccc
      301 gctggtgctc gtggccaccc gccccaagtc cgacgacgtc gacacgtggg agaagcggac
      361 ctcgcagcag caggcgctgg agggcggcga ggacctcgcc accctggcca agggcatggt
      421 cgacaacctc ctgtcgcagg cctccctcga gcggcccgag ctggtcgact acgtgctcgc
      481 cctcatgcag cacaacctgc ccgagggctg ggtcgccgca ctcgaggcga tgaagaaccg
      541 ccccgacgcc atgggggccc tgcgtggcat gccccgcaag accctggttg tggccggtga
      601 gctcgaccgc atcaccccgc tggccgagtc caccctcatc acccgcctcg tcgccgacgg
      661 cgagctggcc gtgatcaagg acagctcgca cctgccgaac ctcgagaacc ccgtcgagtt
      721 caacgaggtg ctgaagcgct tcctcgactc cctcgccgag cccgaggccg aggccgaggg
      781 tgccgacgcc gaagccgagg caccggcagc cgagtagcta cagctcgccc cgcatcgccc
      841 ggtagcggcg gagcagggcg atggtgctcg agtcgtggtc gccgtcggtg ccgtcggcga
      901 tctccgacgc gatcgtggtg gcgagctgct tgcccagctc cacgccccac tggtcgaagc
      961 tgttgatgcc ccacaggaac ccctgggccg cgaccttgtg ctcgtagagc gcgacgagct
     1021 gacccagcac cgacggggtg agccgcggcg ccaggatcgt ggtcgagggc acgttgccgg
     1081 ggaaggtccg gtggggcacg agggacgggg ccacgccgct tgccgcgacc tcctcggcgg
     1141 tgcggccgaa ggcgagggcc tcggcctggg cgagcaggtt cgccgtgagc aggtcgtggt
     1201 gctcggacac gagggagtcg tcggtgggtt cggcgaagcc gatgaggtcg cacggcacaa
     1261 gcgacgtgcc ctggtggagc agctggtaga aggcgtgctg gccgttggtg ccgggctcgc
     1321 cccagacgat ggagccggtg tggtggtcga ccgggtcgcc gtcgagggtg acccgcttgc
     1381 cgttggactc catctccagc tgctggaggt aggcggggaa gcgggcgagc acgtcgctgt
     1441 agggcagcac cgcctgggtg gaggcgccga ggaagttgcg gtaccagagg ttcagcagcg
     1501 ccatgagcac cgaggcgttg tcggcgagcg gggtctctcg gacgtggtcg tcgatgcgcc
     1561 ggaacccctc gagcagctcg ccgaaggcgg cggggccgat gccgatcatg agcgacagcc
     1621 cgatggcgga gtccagcgag tagcggcccc cgacccagtc ccagaagccg aacatgttgg
     1681 cggtgtcgat gccgaacgcc gaaacggcgt cggcgttggt cgacagggcg acgaagtggc
     1741 gctcgacgcg ggtctcgggg ccgaggcgct cgtgcaacca ggccttggcg tgccgggcgt
     1801 tggtgagcgt ctccagcgtc gtgaacgact tggacgcgac gatgaagagc gtcgtctcgg
     1861 gatcgacgtg gcgcagggcc caggccaggt cgtcgccgtc gacgttggac acgaagcgca
     1921 ggtcgaggtc ctcgcgcacc cagtggcgca gcgcggtggc cgccatggcg gggccgaggt
     1981 cggagccgcc gatgccgatg ttgacgacgt gtcggatggg tcgcccggtg gacccggtcc
     2041 actcgccgga gcgcacccgg ctggcgaagg aggagaggcg gtcgagcacc tcgtgcacct
     2101 gggcgacgac gtcgacgccg tcgacctcga gccggtcgtc gcggcgccga cgcagggcgg
     2161 tgtgcagcgc ggggcggtcc tcggtggtgt tgacgcgctc accggtgaag agggcgtgga
     2221 accggccggc cacgtcgcgc tcctcggcca ggcggaccag caggtccagc gtctcggtgg
     2281 tggcccggtg cttggcgaag tcgatgtgga ggtcgccggc ctggaccacc atgcggtcgc
     2341 cgcggccggg gtcgtcggcg aagatctccc gcaggtgcag ctcggccatc cggggctggt
     2401 gcgaggcgag cgcctgccag gccgaggtgc tggtgagcga gctgcgcggc ggggtgtcgg
     2461 ccatgcgccg gagcctaggg ctggggcacg cctgcctcgt ccgctccggg taccgcatcc
     2521 cgggtggcgc ccatccacga gcggtgcatg gcggcgtagg tgcctccgag cgccaggagc
     2581 tcgtcgtggt gcccctgctc gacgaggcgg ccccggtcga acaccagcac caggtcggcc
     2641 agctcggccg tcgagaggcg gtgggcgatg ctgaccaccg tgcggccctg cgacaggtgg
     2701 gccagggcgc cggcgagggc ctgctcggtc tcggggtcga tggcgctggt ggcctcgtcg
     2761 aggatgagca cgcccggctc ggcgagctgg gcgcggccga gcgcgaccag ctggcgctcg
     2821 ccgacggaga ggttgccgcc gcgctcgccg acctcggtgt ggatgccggc aggcaggctc
     2881 cggacccact cgccgaggcc gagcgagtcg aaggcgcgga gcacgtcgtc gtcggtcgcc
     2941 gcctcgacct gcccgtcgcc gcccatgcgg acgttggcgg cgagggtgtc gtcgaagagg
     3001 aagccgtcct gggggaccat gcggatgtgt cgccggcggg cgccgccgcc gacggtggtc
     3061 agttcgacgc cgccgatccg gatcgccccc tcgtcggggt cggcgaggcg gcacaggagc
     3121 ttggcgagcg tggacttgcc ggacccggtc tcgccgacga cggcgacgtt ggtgcccgcc
     3181 gggatgcgga cgtcgacgtg gtggagcacc tggggcccct ctcggtacgc gaacgagacg
     3241 ccccggatct cgatgcccag cggcccgtcg ggcagctcgg cgccgggctc gggatcgtcg
     3301 acgtccaccg gcatgtccag caccccgagg atcttgcgcc agccggcgat ggcggtctgg
     3361 gtctggtcga acacctcgga cagctccgac accggcatga gcaggaggtt gaccaggaac
     3421 aggaacgcga ccagctcgcc gggggtgatg cccagcgcgg cgccctcgag ggagccgacg
     3481 acggcgacgc cggccatggc gaccgagccg aagacgtcgg ccagcgggaa gaggagggcg
     3541 aagaacaggc cggctcgcag gtgggcgcgg tactggccgg tcatggcgga gcgcagccgg
     3601 cgacgggtgc ggtcctcgag cccgtaggcc cggaccaccg ccgatccggt gacggcttcg
     3661 gacacctcgg ccatcgtcga gccgacggtg tcgcggacct cgtcgtaggc ggccagctgg
     3721 cggcgctgga gcgcacgcat caggggcagc agcgggccga aggcgatcag gacgagcacc
     3781 gtcagctgcc aggagtacac gagcatcacc agcagggtcc cgaagatgac cgagctgttg
     3841 acgatccagg cgatggcgcc ccactgggtg aactgggcca gcgtctccac gtcggaggtg
     3901 acccgggcga cgagctcacc ccggcgcgac tcggtgtggt cggcgatggc gaggcggtgg
     3961 atgtggtcga aggtgcggac gcgcagggcg tagagcgcgt cctcggcggt gcggatgagc
     4021 cggaggtagg tggcccgggc gagggcgaac agggccagga tcccgaccac ggccacgatg
     4081 caggccgggt agacgaagcc gggctggaac ccgtcgtcgc ccagcacgcc gcggtcgagg
     4141 atctgctgga tgaggacggg cacggcgagg cggccggcgc cgctcgagac ggccatgccg
     4201 acggtgaacg cgatgccgac cttgagctcg ggggtggcgg cgatgccgcg acgcaggacg
     4261 gcgatggcgc cgtcggtgtc cctgtcggtg tcggtgtcgg tcatgacctc gctcacggct
     4321 gggcccccgg gaggtcgtcg accacggcgt cgccggcctg ctcgtacgcg cgcacgagcg
     4381 cggcgtactg cggatcgcgg gcgagcagct cgtcgtgggt gccgtcgccg acgaggcggc
     4441 caccgtcgag gaacagcacc cggtcggcga ggcggatggt ggccacccgg tgggccacga
     4501 ccaacgtcgt ggtgtcgagg tcctctcgca ggccgtcgag gatgcgctgc tcgatcaccg
     4561 ggtcgatcgc cgaggtggcg tcgtccagga gcagcaggcg ggggtggcgc agcagggcgc
     4621 gggccagggc gagccgttgg cgctgcccgc ccgagagcgt gacgccccgc tcgccgacga
     4681 tggtgtcgcg cccgtgggcg aggtcgtcca cgtagccggc ctgggcgatg tcgatggccc
     4741 actgcagccg ctggtcgccg acgggctcgc cgagggtgag gttctcgagg accgtgtcgg
     4801 cgaacaggaa cgtctcctgg aacacgagcg cgaccgagct gcgcaggtcc atcgggtcgg
     4861 ccaggcggag cgggacgtcg ccgagggcga tctcgcccgt gctcggccgg gcgaggccag
     4921 cgaggaggtt gcacagggtg gacttgccgc tgccggtggc cccgacgatg gcgacgacgc
     4981 cgctcggctc gatgtcgaag ctgatggcgt cgaggacctg ctcgtcgccg tagccgaacg
     5041 acaggtcgcg cacggacagc ggcagcggcc ccggtggcag gcggcggcgt ccgtcgggct
     5101 ccggcgccag cggggcaccg gtgacccggt ggtcgatgtc gaggacgtcg acgatgcggt
     5161 cgcggctcac caccgagcgg ggcagctcct cgagcaagaa cccgatgacc cgcatcggga
     5221 aggccaggat cgcgaacagg accatcgcct ggaccaggtc accggtggtg atcacgtcct
     5281 ccgagacccg ccaggcgccc acggcgagga gggcgaccgt gcccaggttg gggagcgcct
     5341 cgatggcggg ttcgaaggct gcccgcaagc ggccgatgac caggcgctcg cgccggaggt
     5401 ccccggcggc cgtgcgcatc cgggtcgact cccgggcctc gagcccgagg agcttgacga
     5461 cgagcgcgcc ctcgaagctc tcgtgcgcca ccgaggacac gtcgccgacg tgggcctggg
     5521 cgcgcgccga cgggccgacg acgcgggtcg tgtagaggcg gttcatcacg atcagcgatg
     5581 ggaacaggcc gagcccgatc gcgagcagca gcgggtcgag cagggcgagg ttgatcgtgc
     5641 cgaacacgag catcaccacg gcgccgatcg acatcggcag gggctgcatg gcgaacgccg
     5701 agcgctcggc gtcgttgtcg gcgtgcgcca gcagcttgcc ggtgggctgg ccgaagaaga
     5761 aggggagggg ctgctcgacg tagtggtcgc cgaggcggtt gaaccactgc atctgcatgc
     5821 gccgggcggt catgatgccg aagtaccggc gggcgacgac gccggcggac cggaccaccg
     5881 ccatgccgac gatcacgccg cccccgacga gggcggcgcc gacctcgggg cgtccttcga
     5941 cggccggcag gatcaggtcg tcggtgaccc gcccgaggat gatggcgccg gcgaccgacg
     6001 ccgtggcgaa gacgacggcg ccggcgatgg cgccggcggc cggccagggg ttggtgcgca
     6061 ggatctcgac gagcagcgag acgccgcgcc gcatgacgcc cggcgtggtg tcgtgctgcg
     6121 tggcggtggt ctccgccttg tcctccaccg tgggcacggg agcacgctac gacctcgtcc
     6181 ccggccgtcc cgcacccgat ccgcgggccg gctcacctca gggcgatggc cgccacagct
     6241 cgatcggggg gcggtcccgg ggaccgttcc agggcagggc gacctcgtcg ccggtgcggg
     6301 ccgactccga cgccgcgcac acgatctcga gcaggtcccg acccaggtgg acgtcgctca
     6361 ccatcgacgg tcgtcccgcc cgccacgacg cctccgtgtc gcggagctgc tcgacgtagc
     6421 cgtaccggtc cagctgcggt ggggccgggt cgtcgggctc gcgccggggc agcgggggga
     6481 gggcgaccgg ttcgccgttg cgctccaggg cgacctcggg cagcagctcg aagcggacag
     6541 cgccgcggtc cgaggcggcc tgcaggtccc agacgccggt cgggctccgc caggacacct
     6601 cgatctctgc gacgaggccg gagtcgaagc gcagggacac ctcggcgtgg tcctcgacct
     6661 cgacgtcggc gctggcatcg aaccgggccg acacggcgac cggggtggtc cgtgccgccc
     6721 gcaggaccag ggcgagcggg tggaccccga ggtcgaagag gacgcctccg ccccagtggc
     6781 gacgggtgaa gtccccccag gtcgggcggg gctgcagggc ccgtgccgag aggtgccgca
     6841 gcggtccgag gccggcgacc tcgctgagcg cggtcccggt ggcgtcggcg aacaggaggt
     6901 tctcggcata cgtcagcagg ccgccggcct gcacgacccg gaccaggcgg tcggcctcgt
     6961 cgagggtggc gcacagcggc ttctcgacca gcaccgcggc ccccgccgcc atcgcccgga
     7021 gcgcgtcgac cgcgtggcgg gtcggaggcg tggcgaccac gacgatgtcg gcgccggccg
     7081 ggagctcctc gtaggacgcg gcgcggccac cggtctcggc cacccgcgcc tgggcggccg
     7141 cctcggtccg cgacgccacg gcgacgaccc gggagccggg cagggaccgg atcgccaggc
     7201 cgtggatcgc gctgatccac ccggcgccgg cgatggccca gtcggtcatc ggacgtcccc
     7261 gctcatcggg aggaacctag tgagcgcccg tcggggtcgg tgatcgccgg tcccggaggc
     7321 acgccggtac gatcgagcgg tcgtgacccg cgccctgaac tccctcgtgc gcatcgccat
     7381 gctgctcgcg ctcgcctccg tggtggtcac ggcggtgggc atggccgctg ccccgcagct
     7441 caccgggctc accgaggcgg cccacggcga ggccagcgac gtggagctcc gggggttgtc
     7501 gcagcgctcc tacgtgtacg ccgtcgacgg gagcctcatc gccaccctcc aggaggagga
     7561 gaaccgggcg ccgatcacgc tcgacgaggt cccggaccac gtgatcaacg cggtgctcgc
     7621 cgtcgaggac tccggcttct gggatcacag gggcgtgaac gtgcgagcca ccgtgcgtgc
     7681 cctgctgtcc aacgtcgatg ccggcggcac cacccagggc ggctccacca tcacccagca
     7741 gctcatcaag cagagcgtgc tgacctccga gcagaccttc gaccgcaaga cccgggaggt
     7801 cttcctggcg gtgcgcctcg aggagcagat gtccaaggag gagatcctcg agcgctacct
     7861 gaacacgatc tacctcggga accacagcta cggcatccag accgccgccg agacctactt
     7921 caacgtctcg gcgtccgagc tcgatcccgg ccaggccgcg ctgctggccg ggctgatccg
     7981 ggaccccatc gactacaacc cgttccggta ccccgaggtc gctgtcgagc gccgccgcat
     8041 cgcactgagc cggatggtgg ccgagggcca catcaccgag gacgacgcca cctggtacaa
     8101 cgcggtgccg ctgccgaccg agccgagcca gtacacgccc ccgccggacg actacttcgt
     8161 ggaggaggtc aagcaggccc tcctcgccga cccccgcctg ggcgagaccg ccaccgagcg
     8221 ctacgacgcc gtgttccgcg gcggcctgca gatccacacc acgcagaaca tcgggctgca
     8281 gtacctgtcc gaggtcgccc gcaacgacac cctcgccccg ttcgcgctcg agggcgagcc
     8341 cgcgctgttc accgccggac ggaacctggc ggggttcgac gcggtcggca cggtggcgtc
     8401 ggcgacggtg gagccgacca ccggcgcggt gcgggccctc gtcgggggtc ccggcttcgg
     8461 cgaccagtac aagttcaacc tcgcgacgca gggctaccgc cagcccggct cctcgttcaa
     8521 gaccttcgtc ctgctggagc tgctcgagca gggctacgcc ccccaggaca cgatctcggg
     8581 ccgggggccg tgccggttca acctgccgac cgaggaggag ccctacgagg tcgagaactt
     8641 caacaactcc cgtgggttcg tcggctcgat ccgggcgatg accaccgcgt cgtcgaactg
     8701 cggctacgtg cgcctcggcc agatcgccgg aatcgacaac gtcaccgagc tcgccgggga
     8761 gatgggcgtc gtcacccgca acgccgccaa cgagatcgtg ccgctcgacc cctcgttctt
     8821 ctccacgccc ctcggcaccc aggaggtcac gccgctcggc atggccgcgg cgtacgccgc
     8881 gatcgccaac gacggccact accacacccc gtactacgtg gagcgggtgc tcgaccggga
     8941 cggcgagctc atcttccagc acagcgaccc gggcaagcgg gtggtgtcgg tcgagaccgc
     9001 gcagatcgcc gccgacatcc tcaaggagaa cgtcgaatcc ggcaccggga ccgcggctcg
     9061 ggtccggggt cacgacgtcg ccggcaagac cggtaccgcg caggactcct ccaacggctg
     9121 gttcgtcggc ttcagccgcc agctgtccac ggcggtgtgg atgggcgcga tggagggcaa
     9181 cgtcccgatg acccgggtcg gcggccgcag cgtcaccggc gggtcctacc cggcccagat
     9241 cttcggccgg ctgtacaacg cggtgctggc ccagatggag cccgagccct tcgccgagcc
     9301 gccgactcgg cgtggcaccg ggaccctgcg cctcgaccgc aacatcgacc tcgacggcta
     9361 caccacggag ccgacgcgcc ggcgccgcac gaccacgcgc acacccagcc gcacgccgtc
     9421 gcgctccgca cccgccacca ccgcggcgcc ggcgccgacc accacggtcg cccccgcgcc
     9481 gccaccggac cccggcaacg gcaacggcaa cggtggcggt ggcggcggta cgagcgacac
     9541 gacgccgtct ggcgacttcg aggagatgga cggatgaacc cctggaagca gctcctcgac
     9601 gtgcagaccc tcgacaccag cctgacccag ctcgaccacc gggagctcca gctcccggcc
     9661 cggaccgagc tcaccgaggt ggagggtcag ctcgctgcgc tccgcgccga ggtcgcggcg
     9721 gtcgaggggg acaagcacgt cctcgacaag gagcagaagc gcatcgagga cgagatcgcg
     9781 tcgatcgagg acaagatcgc cagcgccgac cgccagctgt acagcgggag ctccgacgtg
     9841 aaggagctgc aggcactcca ggacgagatc gccgccctca agcgccgcat ctccgcgctc
     9901 gaggatcagg agctcgagct gatggagcag gtcgagccca tcgacgcgcg cctcgccggg
     9961 ttcgcggccc gccaggccga gctggacgag caggccgtcg cgctcaccac cgccatcgcc
    10021 gaggccgagg ccgacatcga ccgggagcgc aacgaggtcc ggagccgccg cgacgaggtg
    10081 gtcgccgaga tcgatccgac cgcgctgcag gagtacgaga cggtgcgagg gcgcctcggc
    10141 ggcgtcgccg tggcccggct cgagcacggg tcctgcggtg cctgccacat gaagctgtcc
    10201 gccgtggaga acgaccggat cctgcacctg agccccgacg agccggtccg ctgcgaggac
    10261 tgcggccgct tcctcgtccg cgactgagcc gccacctgtg ctgatctggt acgtggccac
    10321 cgcgctcgcg ggcgtgtggg cggtgttcga cagccccgcg ctcgactacc gcttcgtgat
    10381 ggtgggtgcc gtgctcccgg tgggtgaggt cgtcctcggc cagcccgggc cgctccactc
    10441 cctggtcacc gccgtggcgg tgctggcgct ggtgatggtg gccacccagg gtcggcgcct
    10501 gctgcgccgc cggctcctcg ggatccccat cggcctcctc gcccacctcg tcttcagcgg
    10561 gagctgggcc gacaccgacg ccttctggtg gccgctcacc gggctcgggc tctccgacgc
    10621 cgaaccgccc gagctcgccc gcggtgggct gtccgtcctg ctcgagctgg ccggcatcgc
    10681 gctcgggctg tggtgggtcc ggcgggtcgg cctcgacgac cgagcggctc gggacgagtt
    10741 cgctcgcacc ggtcggctgc cccgggccac cgccccgccg gtgcagggca cgtgctgacc
    10801 ctcgtccgcc acggacgcac ggaggcgaac cgctccgggc tgctgctcgg tcgcatcgac
    10861 gccgagctcg acccgctggg gcgggcgcag gccgatgcag tcgccgcgca cgtgggcccc
    10921 gctgatcggg tcgtgtgcag cccgctggcc cggacccgcg ccacggccgc agcgtgggac
    10981 gcaccggtgg aggtcgacga ccggtggatc gagctcgact acggcgagct cgacggcacc
    11041 ccgatgcggg atgtggacgc cgacctgtgg cggcggtggc ggcgcgacgt cgcc
//
LOCUS       DATISU010000076         6373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_143303, whole genome shotgun sequence.
ACCESSION   DATISU010000076 DATISU010000000
VERSION     DATISU010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143303"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1064)
                     /locus_tag="VLR27_03305"
     CDS             complement(27..1064)
                     /locus_tag="VLR27_03305"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050825 - ice binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HSP02499.1"
                     /translation="MRSRTPRLLGRSLLALGAAALVIGTLPAGAATAVGLGTAAEFSV
                     LAGSAVTNTGPTTVTGSVGVSPGTAISGFPPGIVGGTIHSADAPAAQAQTDTTTAYNT
                     AASQTPATAVAVELGGRTLEPGVYRNGTLGITGTLTLDAQGDPDAVFVFQASSTLITA
                     SGSRVNLINGAQACNVFWQIGSSATLGTSTQFVGTVLALTSITANNGATVQGRLLARN
                     GAVTLDNNVITTAACAAQTTTTTTAPTTTTTTAATTTTTAAPTTTTTTTPAASTPVGT
                     GGTPTGTATPAPVPSGTPTAATPTGGSGGGGGLTTTSLPRTGSGLTALAIGGAVSLLL
                     GAAMVRRAATA"
     gene            1205..1534
                     /locus_tag="VLR27_03310"
     CDS             1205..1534
                     /locus_tag="VLR27_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP02500.1"
                     /translation="MPSWSFLTNHARVLLAIADQPDARLRDLADALDVTERTAFGIVT
                     DLAEAGYLVKERDGRRNRYHIQEHLPLPTDVGREPTIGELLDLLVAAERRPPVDERRR
                     RKGDSTA"
     gene            complement(1547..1954)
                     /locus_tag="VLR27_03315"
     CDS             complement(1547..1954)
                     /locus_tag="VLR27_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HSP02501.1"
                     /translation="MSEPKLLSGGNPQIPKGEGDGPVQDYIAAMPGWKRGLGERLDEL
                     IVRAVPDVHKAVKWNQPFYGNEGDGWFLAFRCYTKYVQLQLFRGTSLDPEPPKASKHP
                     EVRYLDIHEDDDLDEDLIASWVEQASALPGEQV"
     gene            complement(1951..2442)
                     /locus_tag="VLR27_03320"
     CDS             complement(1951..2442)
                     /locus_tag="VLR27_03320"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HSP02502.1"
                     /translation="MITQLFKKKKELHLERSYPAPRDAVWRAWTEADRLRSWWGPPKT
                     TVPECEIDLQIGGRIHVVTEATEEMGKYAGTRWPMEGTFTRIEEAQRLTYDARSWTEG
                     EEATTTIVHTNDVTLTMDGDRTLVRLDVVITDIGPKAKMAAFGMKWGYKAQLDALEEV
                     LAR"
     gene            complement(2439..2765)
                     /locus_tag="VLR27_03325"
     CDS             complement(2439..2765)
                     /locus_tag="VLR27_03325"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSP02503.1"
                     /translation="MQAATFSALGEPSRLQIVELLGLRPYAVGEIAEALGIRQPQVSK
                     HLRVLSESGIVRAERRARRRIYHLQAEPFAEISRWAESFEELWEARLDSLGDYLQTTE
                     DEEHRS"
     gene            2849..3103
                     /locus_tag="VLR27_03330"
     CDS             2849..3103
                     /locus_tag="VLR27_03330"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HSP02504.1"
                     /translation="MARLPLVSSQDPDAPPLAREVLQQINAFRSGSVEPNVYRAVANH
                     PEALQAMVHFGTVVYFNNSMTPAQRELAYLTASVTNECHY"
     gene            3116..3454
                     /locus_tag="VLR27_03335"
     CDS             3116..3454
                     /locus_tag="VLR27_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02505.1"
                     /translation="MLLGRAAGLTEEKLAHLGDDPLPEGVYEPDEAAIVTYAQRSTRL
                     DTIDDGTYGSLREHFDERQIIEICFTVGLSNMINRFHATFLTELDPTTGEALKDSCPL
                     AFPDPSAGGD"
     gene            3480..4631
                     /locus_tag="VLR27_03340"
     CDS             3480..4631
                     /locus_tag="VLR27_03340"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSP02506.1"
                     /translation="MVRGTCDPAFGAVRDAFAANFEAGEVGAACCVSVEGRVVVDIWG
                     GWARTEPRREWAHDTLVNAYSVGKPVIALRLLQLVERGLVDLDDRADGCWPELRAGQQ
                     GATVRDLLCHRAGVPAIRAPLSDDALGDWHAMCDAIAATDPWWEPGTRHAYHTNTYGH
                     LVGEAARRLDGRLPGTWLRDDIAGPLGADLAWGLSAAERARCADVSWEIDVAGAVELM
                     TERARSAETAEQRMIALGYANPPGYSGMGVVNSDAWRAAQVPSTNLHATARGVARLYT
                     ALAAGGALDGVTVLGPALLAEATSAQSVGWCPVLEREATFGLGFQPTRPDRPFGPNPG
                     SFGHFGTGGALGFADPHAGVGFGYVMNSVRPRWQSPRNRALVDALYGCL"
     gene            4648..5487
                     /locus_tag="VLR27_03345"
     CDS             4648..5487
                     /locus_tag="VLR27_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSP02507.1"
                     /translation="MKIANLDGRLVLVVGDGVADVEAASGGRFGPDPMDAFVEWDAFA
                     SWAADVREPTGPLERSRLRCPVPEPRQVFAIGLNYRSHAEESGMAVPEVPATFTKFPA
                     SLSGPHDDIEIVGDTVDWEVELVAIIGAAADRVDEADAWSHIAGLTVGQDISDRTLQF
                     AAGMQFSLGKSRRGYGPMGPWLVTPDEVPDRDDLGLGCRVNGETVQDARTSDLVFSVG
                     ALVAELSSVLPLLPGDVIFTGTPAGVGMGRKPPRALHPGDVLETWIEGIGEMRNTCVA
                     PGT"
     gene            complement(5492..6184)
                     /locus_tag="VLR27_03350"
     CDS             complement(5492..6184)
                     /locus_tag="VLR27_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HSP02508.1"
                     /translation="MRCIALLGGMSWESSIEYERLINEGVRERLGGSHSADLIIRSYD
                     FAAIEELQQADRWDDAGALLAAGARRLQDAGAELVVLCTNTMHVCAPAIEAAIDVPFL
                     HLADATARAVLAAGVRTVGLLGTRYTMERDFYRGRLEQHGLDVLVPDEPDRTTVHDVI
                     FGELVRGVVREESRRSYLDVIERLTAAGAEGVIAGCTEIELLVAADEVAVPWFPTTRL
                     HAQAAVDAALAP"
BASE COUNT          848 a         2224 c         2361 g          940 t
ORIGIN      
        1 ggtggtcgcc ggcgtcctga tccgggctac gccgtcgcgg cccggcgcac catcgcggcg
       61 cccagcagga gcgagacggc gccaccgatc gccagggccg tgaggccgga gccggtccgg
      121 ggcaacgacg tggtcgtgag gccgccgccg ccaccgctgc cgcccgtcgg cgtcgccgcc
      181 gtgggggttc cggacggaac gggggcgggc gtcgccgtgc cggtgggggt gccaccggtg
      241 ccgaccggcg tggacgccgc gggcgtggtg gtcgtggtgg tcgtgggcgc agccgtcgtc
      301 gtggtggtgg ccgcggtcgt cgtggtggtg gtcggcgcgg tcgtcgtcgt ggtcgtctgc
      361 gccgcgcagg cggcggtggt gatgacgttg ttgtcgagcg tgaccgcacc gttgcgggcg
      421 agcaggcgtc cctggaccgt ggcgccgttg ttcgccgtga tcgaggtgag ggcgagcacc
      481 gtgccgacga actgggtgga ggtcccgagc gtcgccgagc tgccgatctg ccagaagacg
      541 ttgcaggcct gggcgccgtt gatcaggttg acccggctgc cgctcgcggt gatgagggtg
      601 ctgctcgcct ggaacacgaa gacggcgtcg gggtcgccct gcgcgtcgag cgtcagcgtg
      661 ccggtgatgc ccagggtccc gttgcggtac acgcccggtt cgagcgtccg gccaccgagc
      721 tcgacggcca cggcggtggc gggggtctgg gatgccgcgg tgttgtaggc ggtggtcgtg
      781 tcggtctgcg cctgggcggc gggtgcgtcc gcgctgtgga tggtgccgcc gacgatgccg
      841 ggcgggaagc cgctgatggc ggtgccgggg ctgacaccga cgctgccggt gacggtggtc
      901 ggccccgtgt tggtgaccgc ggagccggcg agcacggaga actccgctgc cgtgccgagg
      961 ccgacggccg tggcggcgcc ggcgggcagc gtcccgatga cgagggcggc cgcgccgagg
     1021 gcgaggaggg atcggccgag cagccggggg gtgcgggatc tcatgtgggc tctgccttcg
     1081 agagagtcga gccccgagat cggcggccgc cggcggcggg cgatcggtca cggaacgaag
     1141 tcccacggta ctgcgtggcc acaacgatcg caatatgttt ctagtattgg actacattcg
     1201 tggcgtgccg agctggagct tcctcaccaa ccacgcccgc gtgctcctcg ccatcgccga
     1261 ccagcccgac gccaggttgc gcgacctggc cgacgccctg gacgtgacgg agcggacggc
     1321 gttcggcatc gtcaccgacc tcgccgaggc gggctacctc gtgaaggagc gggacggacg
     1381 ccggaaccgc taccacatcc aggagcacct cccgctcccg accgacgtcg gacgggagcc
     1441 caccatcgga gagctgctcg acctcctggt cgcagccgag cgcaggcccc cggtcgacga
     1501 gcgtcgccgg cgcaagggcg actcgaccgc gtgaggcggt gggctgtcag acctgctcgc
     1561 cgggcagggc gctggcctgc tcgacccagg aggcgatgag gtcctcgtcg aggtcgtcgt
     1621 cctcgtggat gtcgaggtag cggacctcgg ggtgcttgga cgccttcggc ggctccgggt
     1681 cgagcgacgt gccgcggaag agctggagct gcacgtactt cgtgtagcag cggaacgcca
     1741 ggaaccagcc gtcgccctcg ttgccgtaga acggctggtt ccacttcacc gccttgtgga
     1801 cgtcggggac ggcgcgcacg atcagctcgt cgagccgctc gcccagaccc cgcttccagc
     1861 ccggcatcgc tgcgatgtag tcctgaacgg ggccgtcgcc ctcgcccttg gggatctgcg
     1921 ggttgccgcc ggagagcagc ttcggctcgc tcatcgcgcc aggacctcct cgagggcgtc
     1981 gagctgagcc ttgtagcccc acttcatgcc gaacgcggcc atcttggcct tggggccgat
     2041 gtcggtgatc acgacgtcga ggcgcacgag cgtccggtcg ccgtccatcg tgagggtgac
     2101 gtcgttggtg tgcacgatcg tggtcgtggc ctcctcgccc tcggtccagg agcgggcgtc
     2161 gtaggtcagg cgctgcgcct cctcgatgcg ggtgaaggtg ccctccatcg gccaccgggt
     2221 gccggcgtac ttgcccatct cctcggtcgc ctcggtgacg acgtggatgc ggccgccgat
     2281 ctgcaggtcg atctcgcact cgggcacggt ggtcttgggc ggtccccacc acgagcgcag
     2341 gcggtcggcc tccgtccacg cccgccacac cgcgtcccgc ggggcggggt aggagcgctc
     2401 caggtgcagc tccttcttct tcttgaacag ctgggtgatc acgatcggtg ctcctcgtcc
     2461 tcggtggtct gcaggtagtc gccgaggctg tcgagccggg cctcccagag ctcctcgaac
     2521 gactcggccc agcggctgat ctccgcgaac ggttcggcct gcaggtggta gatgcgccgg
     2581 cgggcgcgcc gctcggcccg gacgatgccg gactcgctca gcacccggag gtgcttggag
     2641 acctggggct ggcggatgcc gagtgcctcg gcgatctcgc cgacggcgta gggccgcagg
     2701 cccagcagct cgacgatctg cagccggctc ggctcgccga gtgcgctgaa ggtcgctgcc
     2761 tgcatgctcc gaccgtacgg agatgagata ttcctgtcaa ggaatatatg cgttgcgagc
     2821 aggctctggc ccggggttag cctccggcat ggctcgtctc cccctggtca gctcccaaga
     2881 cccggacgcc ccgccgctcg cccgcgaggt gctccagcag atcaacgcct tccgcagcgg
     2941 ctcggtcgaa cccaacgtgt accgagcggt ggccaaccac ccggaggcgc tgcaggccat
     3001 ggtccacttc ggcacggtcg tgtacttcaa caactccatg acccccgccc agcgggagct
     3061 ggcgtacctg accgcgtcgg tcaccaacga gtgccactac tgaatcccga cgcacgtgct
     3121 gctcggtcga gcagcgggac tgacggagga gaagctggcg cacctgggcg acgatccgct
     3181 gcccgagggc gtctacgagc cggatgaggc ggcgatcgtc acctacgccc agcgctcgac
     3241 gcggctcgac accatcgacg acggcaccta cgggtcgctc cgcgagcact tcgacgagcg
     3301 gcagatcatc gagatctgct tcaccgtcgg cttgagcaac atgatcaacc ggttccacgc
     3361 cacgttcctg accgagctgg acccgacgac gggtgaggcg ctgaaggaca gctgccccct
     3421 ggccttcccc gatccatccg ccggcggcga ctgaccgacc gcgggcgtac ggtcgggccg
     3481 tggtccgggg tacctgcgat ccggcgttcg gcgcggtgcg ggatgcgttc gcggccaact
     3541 tcgaggcggg ggaggtcggc gcggcgtgct gcgtctccgt cgagggccgg gtggtcgtcg
     3601 acatctgggg cggatgggcc cgcaccgagc cccggcggga gtgggcccac gacacgctgg
     3661 tgaacgccta ctcggtgggc aagccggtga tcgcgctccg gctgctccag ctggtcgagc
     3721 gcggcctggt cgacctcgat gaccgagcgg atggctgctg gcccgagctg cgggccggcc
     3781 agcagggcgc gacggtccga gacctgctct gccaccgcgc cggcgtcccg gcgatccgcg
     3841 caccgctcag cgacgatgcg ctcggcgact ggcacgccat gtgcgacgcc atcgcggcaa
     3901 cggacccgtg gtgggagccc gggacgcggc acgcctacca cacgaacacc tacggccatc
     3961 tcgtggggga ggccgcccgg cgcctcgacg gacgcctgcc cggcacgtgg ttgcgggacg
     4021 acatcgccgg cccgctcggt gccgacctgg cctgggggct cagcgccgcc gagcgcgccc
     4081 ggtgcgccga cgtgtcgtgg gagatcgacg tcgccggtgc ggtcgagctg atgaccgagc
     4141 gcgcccggag cgccgagacg gccgagcagc ggatgatcgc cctcggctac gcgaacccgc
     4201 cgggctactc ggggatgggc gtggtgaaca gcgacgcgtg gcgcgccgcc caggtgccct
     4261 cgacgaacct gcacgcgacc gcgcgtgggg tggcccgcct ctacaccgcg ctggctgcag
     4321 gcggcgcgct cgacggggtc accgtcctcg gacccgccct gctggccgag gccacgtcag
     4381 cccagtcggt ggggtggtgc ccggtcctcg agcgagaggc gacgttcggc ctcggcttcc
     4441 agcccacccg gcccgaccgg cccttcggac cgaacccggg cagcttcggc cacttcggga
     4501 ccggtggtgc gctgggcttc gccgacccgc atgccggcgt ggggttcggc tacgtgatga
     4561 actcggtccg gccccgttgg cagagcccac gcaaccgggc gctcgtcgac gccctgtacg
     4621 gctgcctgtg atgcggagga gaccccgatg aagatcgcca acctggacgg ccgcctcgtg
     4681 ctcgtggtcg gtgatggcgt cgccgacgtc gaggccgcat ccggcggccg cttcggcccc
     4741 gacccgatgg acgccttcgt cgagtgggac gccttcgcct cctgggccgc ggacgtgcgc
     4801 gagccgacgg ggccgctgga gcggtcccgg ctgcgctgcc cggtgcccga gccgcggcag
     4861 gtgttcgcga tcggcctcaa ctaccgcagc cacgccgagg agtcgggcat ggcggtcccc
     4921 gaggtcccgg cgacgttcac caagttcccg gcgtcgctca gcgggcccca cgacgacatc
     4981 gagatcgtcg gcgacaccgt cgactgggag gtcgagctcg tcgccatcat cggcgcggcg
     5041 gccgatcggg tggacgaggc cgacgcgtgg tcccacatcg ccgggctgac cgtcgggcag
     5101 gacatcagcg accggacgct gcagttcgcg gccggcatgc agttctcgct cggcaagtcc
     5161 cggcgcggct acggccccat gggcccctgg ctcgtcacgc ccgacgaggt ccccgaccgc
     5221 gacgacctcg gcctcggctg ccgggtcaac ggggagaccg tgcaggacgc ccgcaccagc
     5281 gacctcgtct tctcggtcgg cgccctggtg gccgagctgt cgtccgtcct gccgctgctg
     5341 cccggcgacg tgatcttcac cgggacgcct gccggcgtgg ggatgggccg caagccgccg
     5401 cgagccctgc atcccggcga cgtgctcgag acgtggatcg agggcatcgg cgagatgcgc
     5461 aacacctgcg tggcgccggg gacctgaccg atcagggagc gagggccgcg tcgaccgcgg
     5521 cctgggcgtg gaggcgcgtc gtggggaacc acgggacggc cacctcgtcg gcggcgacca
     5581 gcagctcgat ctcggtgcac ccggcgatga cgccctcggc acccgctgcg gtcagccgct
     5641 cgatcacgtc caggtaggag cgcctcgact cctcccgcac cacgccccgg accagctcgc
     5701 cgaagatgac gtcgtggacc gtcgtccggt ccggctcgtc gggcacgagc acgtcgaggc
     5761 cgtgctgctc gaggcgaccc cggtagaagt cgcgctccat cgtgtagcgg gtgccgagca
     5821 gccccaccgt gcggacgccg gccgccagca cggcgcgggc ggtggcgtcg gccaggtgga
     5881 ggaagggcac gtcgatggct gcctcgatgg ccggggcgca cacgtgcatg gtgttcgtgc
     5941 acagcacgac gagctccgcg ccggcgtcct ggaggcggcg ggcgccggcg gcgaggaggg
     6001 cgccggcatc gtcccaccgg tcggcctgct gcagctcctc gatggctgcg aagtcgtagc
     6061 tgcggatgat caggtcggcg gagtggctcc cgccgagccg ctcccgcacc ccctcgttga
     6121 tcagccgctc gtactcgatc gagctctccc agctcattcc gccgagcagg gcgatgcaac
     6181 gcatcagcag aacgtagacg gaccgaccta ctgtcaccgg ggtgtcggcg aaccccgacg
     6241 tcgatcagta cctcgagcgc tccgagacgt ggccggacga ggtcgccgcg ctgcgtcccg
     6301 tcctgctcgg gtgcgggttg accgagcagc tcaagtggtc caagccctgc tacagccacg
     6361 acgggaacaa cat
//
LOCUS       DATISU010000077         4684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_655518, whole genome shotgun sequence.
ACCESSION   DATISU010000077 DATISU010000000
VERSION     DATISU010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655518"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VLR27_03355"
     CDS             <1..1068
                     /locus_tag="VLR27_03355"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HSP02509.1"
                     /translation="HAFSESDIGGWLLGFFGLIVVVTVGLIGWRGDRLRAPGSMDSPI
                     SREGSFLANNVLFAAFTFVVLLGTVFPLVVEAVNGEQLSVGVPYFNRMTMPLGMLLLF
                     LMAIAPVLPWRKASQELLRVRLFWPAVAATVTVVATVVAGARGLAPVLAFGLGAFAGG
                     AAIRQVVLASRRQGWRGVVGRTNGGMIVHLGVVMIAVGLAASQSYSSEVELRMEEGQV
                     ATVDGHTLVFEGLETREDPEKTSISALVRVDGGKVYRPAINEYVFGGQTIGTPSVSTG
                     LRQDVFLALLDVPEEEGDPVVIRVVIQPLVTWLWIGGGVMGLGTALAAFPGKRRRPTQ
                     PTSASGTTEPDPEPEMVGASA"
     gene            1065..1667
                     /locus_tag="VLR27_03360"
     CDS             1065..1667
                     /locus_tag="VLR27_03360"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSP02510.1"
                     /translation="MTAPARERRRIGPWIAGAVLVVLAAFIGVLATGGDDDGVRSTLI
                     GRPAPGISGETIDDATYDLADHRGEFVVVNYFATWCVPCIREHPELVEFEERHRETGD
                     ASVVSVVFDSRPQQVREFFAEYGGEWPVVLDPDGRTALEYGVTGVPESYLVAPDGTVL
                     LKIEGGVTAEALDDLLEQAQVSDVEGEHLDDEGDDEADAS"
     gene            1664..2134
                     /locus_tag="VLR27_03365"
     CDS             1664..2134
                     /locus_tag="VLR27_03365"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HSP02511.1"
                     /translation="MKDLSRARWPWILLVALLAGGLVVAAIGDGGPRTSAERARAVSE
                     SIKCPTCQGQSVADSSAPAARAVRTEIARRIEAGETDQQIRDYISGIYGDDALLTPPR
                     DGVAGLIWFLPVAGLVAAVGGLVVVFRRWRVPEDVEVSDEDRVLVEQARRASRS"
     gene            2131..3234
                     /locus_tag="VLR27_03370"
     CDS             2131..3234
                     /locus_tag="VLR27_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02512.1"
                     /translation="MTLDADERAALEEERDFLLASLDDLERERAAGDVDDHDYRTLKA
                     DYTARAAAVLRSLDPAATDATSPVPDAVPGRRGVSSRGRRIATIAGIALVAVLAGVLV
                     AQASGRRGSGSLTGLDVTAASSRIDDCQELEQAGDADGSLGCYDEVLDAVPANVEALT
                     FRGWLQVRQFEVEDGLSDLDAAIQLAPDATAPYIFRASGRSRSGDAPGAVADLAAFYA
                     NGPDEDEAQLADQFSVTIVDAALDACIDGDVNGTLPVADVLQCYTDILEVDEGNAAAS
                     IYLGWLIARSGVDGDMALQRLDDGLAADPSVSAGYVFRAALRAHLGDIEGARADLDAF
                     AGTDAPEAQQAAADQVRAAIDAGEDPLPARASG"
     gene            complement(3241..3732)
                     /locus_tag="VLR27_03375"
     CDS             complement(3241..3732)
                     /locus_tag="VLR27_03375"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HSP02513.1"
                     /translation="MTLYVVRHAHAGRRSEWTGDDRTRPLSDRGELQAKGIATAIDQD
                     RTHARLRILTSPAIRCGQTVQPLANQVGEDVRVEDRLFEGADRDQVGSLLDEVTGAVD
                     DGSDVVVCSHGDVIPMLLDLLVQAGMEPERNLVWQKASMWVVPRDDGRWGTGRYLPPP
                     DRA"
     gene            3782..4399
                     /locus_tag="VLR27_03380"
     CDS             3782..4399
                     /locus_tag="VLR27_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HSP02514.1"
                     /translation="MGFFDRNRRQLEARGIDPDRLPPGQYATDRFPVLHVGDVPRYAD
                     DLSDWDLRLTGGVANPTTLTFDELRSLPTVEETFDIHCVTKWSKFDTRWTGVRLTTLM
                     DIAGVHPDATHLIEHAEFGYTTNLPLAEVRRDDVLVAWAFDGEPLEPEHGYPVRLVLP
                     HRYFWKSAKWLRALEFSAVDQPGFWERNGYHNDGDPFLEQRRWGD"
BASE COUNT          589 a         1789 c         1626 g          680 t
ORIGIN      
        1 cacgccttct ccgagtccga catcggcggg tggctgctcg gcttcttcgg cctcatcgtc
       61 gtcgtcacgg tcggcctcat cggctggcgc ggcgaccgtc tccgggcgcc cggcagcatg
      121 gactcgccga tctcgcgcga gggctccttc ctcgccaaca acgtgctctt cgccgccttc
      181 accttcgtgg tgctgctcgg caccgtcttc ccgctcgtgg tcgaggcggt gaacggcgag
      241 cagctgtcgg tcggcgtgcc gtacttcaac cggatgacca tgccgctcgg catgctgctc
      301 ctgttcctga tggccatcgc gccggtgctg ccgtggcgca aggcgtccca ggagctgctg
      361 cgggtccggc tgttctggcc cgccgtcgcc gccaccgtca ccgtggtcgc cactgtggtc
      421 gccggcgccc ggggcctggc cccggtgctc gccttcggcc tcggcgcctt cgccggcggt
      481 gctgccatcc gccaggtcgt cctcgcctcc cgccgccagg gctggcgggg cgtcgtcggc
      541 cgcaccaacg gcggcatgat cgtgcacctc ggcgtcgtga tgatcgccgt cggcctcgcc
      601 gcctcgcagt cgtactcgtc cgaggtcgag ctgcggatgg aggagggcca ggtcgccacc
      661 gtcgacggcc acaccctcgt gttcgagggc ctcgagaccc gggaggaccc ggagaagacc
      721 tcgatctccg ccctcgtgcg ggtggacggc ggcaaggtct accgcccggc gatcaacgag
      781 tacgtcttcg gcggccagac catcggcacg ccgtcggtct cgaccgggtt gcgccaggac
      841 gtgttcctcg ccctgctcga cgtgccggag gaggagggcg acccggtggt catccgcgtc
      901 gtcatccagc ccctcgtcac ctggctgtgg atcggcggcg gcgtgatggg cctcggcacg
      961 gcgctggccg ccttcccagg caagcgtcgt cgcccaacgc agcccacgtc ggcatccggg
     1021 acgacggagc ccgacccgga gcccgagatg gtcggagcgt cagcgtgacc gccccggccc
     1081 gcgagcggcg acgcatcggc ccctggatcg ccggtgccgt gctggtcgtg ctggccgcct
     1141 tcatcggcgt gctggccacc ggtggggacg acgacggcgt gcgctccacg ctcatcggac
     1201 gcccggcccc cggcatctcc ggcgagacca tcgacgacgc cacctacgac ctcgcggacc
     1261 accggggcga gttcgtcgtg gtcaactact tcgccacgtg gtgcgtgccg tgcatccgcg
     1321 agcatcccga gctggtcgag ttcgaggagc gccaccgcga gaccggcgac gcctccgtgg
     1381 tgtcggtggt cttcgacagc cgcccccagc aggtccggga gttcttcgcc gagtacggcg
     1441 gcgagtggcc cgtcgtcctc gaccccgacg ggcggaccgc gctcgagtac ggcgtcaccg
     1501 gcgtgcccga gtcctacctg gtcgcgcccg acggcaccgt gctgctcaag atcgagggcg
     1561 gcgtgaccgc cgaggcgctc gacgacctcc tcgaacaggc ccaggtctcc gacgtggagg
     1621 gcgagcacct ggacgacgag ggcgacgacg aggcggacgc atcgtgaagg acctgagccg
     1681 ggcccgctgg ccgtggatcc tgctggtcgc gctcctcgcc gggggcctgg tggtcgccgc
     1741 catcggcgac ggcgggcccc gcaccagcgc cgagcgcgct cgcgccgtgt cggagtcgat
     1801 caagtgcccg acctgccagg ggcagtccgt ggccgactcg tctgcgcccg ctgcccgggc
     1861 ggtgcgcacc gagatcgccc ggcgcatcga ggccggcgag accgaccagc agatccgcga
     1921 ctacatctcg ggcatctacg gcgacgacgc cctgctcacc ccgccccgcg acggcgttgc
     1981 cggcctgatc tggttcctcc ccgtcgccgg cctcgtggcg gctgtcggcg ggctggtcgt
     2041 ggtgttccgg cgctggcggg tgcccgagga cgtcgaggtg tccgacgagg accgcgtgct
     2101 ggtcgagcag gcccgacgcg cctcccggtc gtgaccctgg acgcggacga gcgggcggcg
     2161 ctggaggagg agcgggactt cctcctcgcc tcgctcgacg acctcgagcg cgagcgggcc
     2221 gccggcgacg tcgacgatca cgactaccgc acgctcaagg ccgactacac cgcacgggcg
     2281 gccgccgtcc tgcggtccct ggaccctgct gccacggacg cgacgtcgcc ggtgcccgac
     2341 gccgtcccgg ggcggcgcgg ggtctcgtcc cgaggtcgtc gcatcgccac catcgccggc
     2401 atcgccctgg tcgcggtgct ggccggcgtg ctcgtcgccc aggcgtcggg gcgcaggggg
     2461 tcgggcagcc tcaccggtct cgacgtgacc gccgcgtcca gccgcatcga cgactgccag
     2521 gagctggagc aggccgggga cgccgacgga tcgctcggct gctacgacga ggtgctcgat
     2581 gccgtcccgg ccaacgtcga ggcgctgacc ttccgcggct ggctccaggt ccgccagttc
     2641 gaggtcgagg acgggctctc cgacctcgac gccgccatcc agctggctcc ggacgccacc
     2701 gccccctaca tcttccgtgc cagcggtcgg tcccgcagcg gagacgcccc cggtgccgtg
     2761 gccgacctcg ctgccttcta cgccaacggc cccgacgagg acgaagccca gctcgccgac
     2821 cagttctcgg tgacgatcgt cgacgccgcg ctcgacgcct gcatcgacgg cgacgtcaac
     2881 ggcacgctgc cggtcgccga cgtgctccag tgctacaccg acatcctcga ggtcgatgag
     2941 ggcaacgccg cggcgagcat ctacctgggg tggctcatcg cccgcagcgg ggtcgacggc
     3001 gacatggccc tgcaacgcct cgacgacggc ctcgccgctg acccgtcggt ctctgccggc
     3061 tacgtgttcc gggccgccct gcgcgcccac ctgggtgaca tcgagggcgc tcgcgccgac
     3121 ctggacgcgt tcgccggcac cgacgccccc gaggcgcagc aagccgcggc cgaccaggtg
     3181 cgagcggcca tcgacgccgg ggaggacccc ctacccgctc gtgcgagcgg gtagcggccc
     3241 tcaggcccga tccgggggtg gcaggtagcg ccccgtgccc caacgcccgt cgtcgcgggg
     3301 gacgacccac atcgatgcct tctgccagac caggttccgc tccggttcca tcccggcctg
     3361 gaccaggagg tcgagcagca tcgggatcac gtcgccgtgg ctgcacacca cgacgtccga
     3421 gccgtcgtcg acggcgccgg tgacctcgtc gagcagcgag ccgacctggt cgcggtccgc
     3481 cccctcgaac agccggtcct cgacgcgcac gtcctcgccg acctggttgg cgagcggctg
     3541 cacggtctgg ccgcagcgga tggcgggtga ggtcaggatg cggagcctgg cgtgcgtccg
     3601 gtcctgatcg atcgccgtgg cgatcccctt ggcctggagc tcgccacgat ccgagagcgg
     3661 tctggtccgg tcgtcaccgg tccattccga tcggcggccg gcgtgggcgt ggcggaccac
     3721 gtagagcgtc acggctgcgc acgctattgc ctcgcccggg ccacggctac cgtcgcctgc
     3781 cgtggggttc ttcgaccgca accgccggca gctcgaggcc cggggcatcg acccggatcg
     3841 gctcccgccc ggccagtacg ccaccgaccg cttcccggtc ctgcacgtcg gggacgtgcc
     3901 ccgctacgcc gacgacctgt ccgactggga cctgcgcctc accggcggcg tggccaaccc
     3961 cacgacgctg accttcgacg agctccggtc gctgccgacg gtcgaggaga ccttcgacat
     4021 ccactgtgtg acgaagtggt ccaagttcga cacccgctgg acgggtgtcc ggctcaccac
     4081 cctgatggac atcgccggcg tgcaccccga cgccacacac ctcatcgagc acgccgagtt
     4141 cggctacacc accaacctgc cgctggccga ggtccggcgg gacgacgtcc tggtcgcctg
     4201 ggccttcgac ggcgaaccgc tcgagcccga gcacgggtac ccggtgcggc tggtcctgcc
     4261 gcaccggtac ttctggaagt cggcgaagtg gctgcgggcg ctggagttct ccgccgtcga
     4321 ccagccgggg ttctgggagc gcaacggcta ccacaacgac ggcgatccct tcctcgagca
     4381 gcgccgctgg ggcgactgag cacgcctgct gctccgagtc ggctgtcaga cccccgccat
     4441 agcgttcgag gtgggtcgga gcgccgccct cgctcccgtc acgtcgcagg tccacctgcc
     4501 cgacgcgggg agccaccgtg tcccatccac catccgatcg cttccggtac cgggggtcgg
     4561 aaccgccccg accgccgcgt cgcccacctg gcggcctccg cgccgagcgg caccgccccc
     4621 gggcccgccg ccggaccttc gtcgaggcgc gagcgctgca cgcggacaac accgagcggc
     4681 tgcg
//
LOCUS       DATISU010000078         7656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_655570, whole genome shotgun sequence.
ACCESSION   DATISU010000078 DATISU010000000
VERSION     DATISU010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655570"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..356
                     /locus_tag="VLR27_03385"
     CDS             159..356
                     /locus_tag="VLR27_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02515.1"
                     /translation="MAVLAVAPTLIACRNRMRPDRPPAPHFLPTLHPLFLALELAQDP
                     ARGREVVEQWNLDHAEVDRVW"
     gene            331..1095
                     /locus_tag="VLR27_03390"
     CDS             331..1095
                     /locus_tag="VLR27_03390"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HSP02516.1"
                     /translation="MPRSIVSGDLDEILLADHHHELVEAARAVLATSDLPPVVLIGGL
                     AVAMRVGSTGQPHRATVDIDLVTTETNPAAVEILADAHDSARHPLVIEGVKVDLIATM
                     EASATDLADLDDDDRLFVAGHRWAFETGVLTVVRVAGGEPLPAQVATPAGLVATKSHA
                     IAAPSTRRRSTKMPADLLDLYRLVERYDRRGAVSQEIRTVPGEIAAVIATVVEREILA
                     NPAAATNKMRSASLSPVDPVEVVDVMETFVEDLRRV"
     gene            complement(1344..2435)
                     /locus_tag="VLR27_03395"
     CDS             complement(1344..2435)
                     /locus_tag="VLR27_03395"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSP02517.1"
                     /translation="MGDADHFARHCWGRAPQLHRTGDDFADVLTLAEVDRLLSSTARR
                     PEVRVMRAGEPVDAASWCTTVRLGGRQVPDVVDAVAIGRALGDGATVVLQSLHRTVPA
                     VGRFAAALEAEVSHPVQVNAYLTPPGAAGLAPHADGHDVIAVQLHGTKAWTVDGLGDV
                     TLEPGDALYLPAGTRHAAETNERSSLHLTIGIIRITYRAAIQRLLAQATDLDAPLPLG
                     YATPDADRAELAAGLTGAIGSAATALSEADPADLAEAEHRRRRPRLRHEGHVSSVVAL
                     DELGPGSCVCVRPGPPPALSTEDDGRLRLDLGDRILHLPAVAGPALELLLDGGVVAVG
                     NLPDLDEESRLVLARRLVREGMLLVDPPC"
     gene            complement(2508..2681)
                     /locus_tag="VLR27_03400"
     CDS             complement(2508..2681)
                     /locus_tag="VLR27_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02518.1"
                     /translation="MTNQLTDDQITASGPTEDDDNLDAPGEHTKDTGDEPTEDDENLD
                     APGEDSKDTGDEA"
     gene            2742..3401
                     /locus_tag="VLR27_03405"
     CDS             2742..3401
                     /locus_tag="VLR27_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HSP02519.1"
                     /translation="MATPEIATTYLAARQRIDDLVRPLDDDDLSRPVPTCPGWTVHAV
                     VSHLAATPDWAAAGRLTGLPSDEDTAAQVAELADTPTGEVLDRWATTAPGFAELAAGA
                     DIWPAAIDIVTHEHDIRHALGRPGERDTEAVGTLAEALLAWWSPSRAVEVVTPARAVR
                     CGPADQEPLRWTSTDFEVLRARLGRRSRAQLAAMDWSDDPGELLDELTVFPPSGTDID
                     E"
     gene            complement(3423..4187)
                     /locus_tag="VLR27_03410"
     CDS             complement(3423..4187)
                     /locus_tag="VLR27_03410"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HSP02520.1"
                     /translation="MNEHDEIAELLGAYALDAVDADEAATVEAHLAVCPRCAAEVADH
                     REVAAMLAHSGAPAPEGLWTRISASLEEAPPEMDLRLTRPGPAPDDVVDLAERRQRRS
                     VPRWLPGAAVAAALVVVALVAGLVIGGGDAERDPDVQIALTDVARDVMNDPDATRVVL
                     RSPDGGELEAPAAIDRDGAGYLMGNTLPALDDSETYQLWGIQGDLVVSLGVLGPSPEV
                     VAFRADPALDALAITQEVAPGVVSSENPALLVGEVS"
     gene            complement(4184..4762)
                     /locus_tag="VLR27_03415"
     CDS             complement(4184..4762)
                     /locus_tag="VLR27_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP02521.1"
                     /translation="MTIEQLQEASDAALVVSIGRYHEDALAEVYRRHAGAVFALARRL
                     LADRTLAEEVVQEVFLRLWNNPDRFDPDRGSLRSYLLAQCHGRSVDLIRSESSRRRRE
                     EKELTLAAEAGYDLEHEVWDMAVADRVKVTLQSLPEGEREAILLAYFGGHTYREVADL
                     LGTPEGTVKSRIRSGLKKMRGELAGIGIGGGA"
     gene            complement(4813..6006)
                     /locus_tag="VLR27_03420"
     CDS             complement(4813..6006)
                     /locus_tag="VLR27_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HSP02522.1"
                     /translation="MHDLELRVDDVAVGGDGIARADDGRVVFVRGGVPGDVLTAVVTK
                     EERRLLKADAVTIRTASDDRAVPPCPHVADGCGGCGWQHLTVDAQRRWKQRMVEESLR
                     RLGRQEGSVSLAPPLPDRGFRTSLRLLVTPAGPAFRGARSHDAVPIASCMVAHPGLDE
                     LIRDGRFGAADEVTLRIGGSTGERLAVVAPGIPDDLALPPDVIVVGEDELAEGRHTWF
                     HDEVAGRRFRISARSFFQTRTDGAEALVAAVRDAGGDAWGHGRLADLYGGVGLFASTL
                     GDGMSTVLVESARSSVADAEDNLADRDATVVRSSVDRWRPSPADLVVADPPRAGLGKA
                     ALAPILATGARRLVLISCDAAAFGRDAGLLAEAGFERRSTVLVDLFPHTPRVELVSRF
                     DRKVA"
     gene            6072..6767
                     /gene="rpe"
                     /locus_tag="VLR27_03425"
     CDS             6072..6767
                     /gene="rpe"
                     /locus_tag="VLR27_03425"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HSP02523.1"
                     /translation="MSDRPGTDRPILIAPSVLPADFSRLGDAVTALDEAGVDRIQWDV
                     MDGRFVPNLTFGPDVIASCRDRTELLFEAHLMVEDPDPLLHTWIDAGCGLVIVHAETC
                     RHLHRTLGRIKDLGAGAAVALNPSTPPEVVEHVLDLCDMVLVMTVNPGFGGQRYIETM
                     EPKVATIRRWIIDGGHDCDIEVDGGISPETVRGPVAAGANVVISGSSMFKDPDGLGHA
                     VDELRRLAEAARA"
     gene            6767..7183
                     /locus_tag="VLR27_03430"
     CDS             6767..7183
                     /locus_tag="VLR27_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02524.1"
                     /translation="MAHRLPAAFLVAALAVVGAACNDDDGSGNLAAFCEAAADTQRFE
                     TVFDDLDPTNVEAARETFRTARDAQEGLRELAPEAARADIDVVIAFVDDLIVGLQPGR
                     DVDEHGRPSVYQGLRPRFDEVEAAGDRLRVYVESNC"
     gene            complement(7207..>7656)
                     /locus_tag="VLR27_03435"
     CDS             complement(7207..>7656)
                     /locus_tag="VLR27_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HSP02525.1"
                     /translation="LYLEGQDPDKDIWLYINSPGGVITAGMAIYDTMQFISPDVATIC
                     MGLAASMGQFLLTAGAEGKRYTLPNARILMHQPLGGVQGQASDIAIQAEQLAFINKLM
                     AERIAEHTGQTVEQIQADSERDRWFTAEQALEYGIVDKVITRRGETD"
BASE COUNT         1064 a         2764 c         2789 g         1039 t
ORIGIN      
        1 tggacgagcc tggctgggcg aatggtggcg atgccgcggc ggcagcgtgg ggcgcgccgg
       61 tcttcgaggc cggatcccga cctcggatct gggtcccgac ggacgcggtc gtgcggcgag
      121 ccgaacggac actggaacgg gcttcgtggg atgactgtgt ggcggtgctg gcggtggcgc
      181 cgacgctcat cgcctgccgc aatcgcatgc ggcccgaccg gcctccggca ccgcacttcc
      241 taccgaccct ccacccgctg ttcctcgccc tcgagttggc ccaggacccg gcacgcggcc
      301 gtgaggtcgt cgagcagtgg aacctcgacc atgccgaggt cgatcgtgtc tggtgatctc
      361 gacgagatcc ttctcgccga ccatcaccac gagctggtcg aggctgcgag agcagtcctc
      421 gccacgtccg acctcccgcc ggtcgtgctc atcggcgggc tagccgtggc catgcgcgtc
      481 ggctcaaccg gccaaccgca tcgagcgacc gtcgacatcg acctcgtcac taccgaaacg
      541 aatccggcag ccgtcgagat cctcgccgac gcccacgact cggcccggca cccactggtg
      601 atcgagggag tcaaagtcga tctgatcgcg acgatggagg cgagcgccac cgatctcgcc
      661 gacctcgacg acgacgatcg cctgttcgtc gccgggcatc ggtgggcgtt cgagaccggg
      721 gtcctcaccg tcgtccgagt cgccggcgga gaacccctgc cggcgcaggt cgccacgccg
      781 gcaggcctcg tcgccaccaa gtcccatgcg atcgccgcgc cgtcgacgcg ccggcggtcg
      841 accaagatgc cggccgacct cctcgatctc taccgtctcg tcgagcggta cgaccgccgc
      901 ggtgcggtca gccaggagat caggaccgtg ccgggcgaga tcgccgcggt gatcgctacg
      961 gtcgtcgagc gagagatcct tgccaacccc gcagcggcga ccaacaagat gcggtcggct
     1021 tcactgtcgc ccgtcgatcc cgtcgaggtc gtcgatgtca tggagacctt cgtcgaggac
     1081 ctgcgacggg tctgactcca cgtctgcgat ccccccgatc gtccccccaa atcagcgata
     1141 cgggccagta cgggggatca caggacaaca gcgccacgac tgaaaacccg gatccaccag
     1201 gactttcgcg cactcgacgt catcagccgg acggccgtca agggactctt aatccgcagg
     1261 ttctgggttc gagtcccagg gggggtacag gagcgccggg ccagggcacg tccctggccc
     1321 ggggcgcgtc cgggcagtgg cgctcagcag ggcggatcga cgaggagcat gccctcgcgc
     1381 acgagccgtc gggccagcac cagccggctc tcctcatcga gatcgggcag gttgcccacc
     1441 gccaccacgc cgccgtccag caggagctcg agcgcaggac cggccacggc cggcaggtgc
     1501 aggatgcggt cccccaggtc gaggcgcagg cggccgtcgt cctcggtcga cagggctggt
     1561 ggcggacccg gccggacgca cacgcaggac ccgggcccga gctcgtcgag tgcgaccacg
     1621 gacgagacgt ggccctcgtg gcgcaggcgg ggacggcgtc gacggtgctc ggcctcggcc
     1681 aggtcggcgg ggtcggcttc cgagagcgcc gtcgccgcgc tcccgatggc tccggtcaga
     1741 ccggcggcca gctcggcacg gtccgcgtcg ggggtggcgt agccgagcgg gagcggcgca
     1801 tcgaggtcgg tcgcctgggc gagcagtcgc tggatcgcgg cccggtaggt gatccggatg
     1861 atgccgatcg tcaggtgcag cgacgaccgc tcgttcgtct cggcggcgtg gcgggtgccg
     1921 gccggcaggt acagggcgtc gccgggttcg agcgtgacgt cgccgaggcc gtcgacggtc
     1981 caggccttcg tgccgtggag ctggacggcg atgacgtcgt ggccgtcggc gtgcggggcg
     2041 agcccggcgg ctccgggcgg ggtcaggtag gcgttgacct gcaccgggtg ggagacctcg
     2101 gcttccagcg ccgccgcgaa gcgacccacg gcgggcacgg tgcggtgcag cgactgcagc
     2161 accaccgtgg cgccgtcgcc gagggcgcgc ccgatggcga cggcatccac cacgtcgggc
     2221 acctggcggc cacccaggcg gacggtggtg caccacgagg cggcatccac cggctcgccg
     2281 gcgcgcatca cccggacctc cgggcgccgg gccgtgctcg acagcaggcg gtcgacctcg
     2341 gccagggtca ggacgtcggc gaagtcgtca ccggtgcggt gcagctgggg cgcacgaccc
     2401 cagcagtgcc gggcgaagtg gtccgcgtcg cccacgcagc gcgccagcac cggccgtcgc
     2461 gcaccgtgcg cggggccggc aggcgtcccg ttgtgcgggg ggatgggtca ggcctcgtcg
     2521 ccggtgtcct tggagtcctc gccgggggcg tcgaggttct cgtcgtcctc ggtgggctcg
     2581 tcgccggtgt ccttggtgtg ctcgccgggg gcgtcgaggt tgtcgtcgtc ctcggtgggg
     2641 ccggaggcgg tgatctggtc gtcggtcagc tggttggtca tggggcgtcc cttcccaact
     2701 cgcccgcagc cagcaccgcg cgggtgccat gctgcgcggc catggccacc ccggagatcg
     2761 ccacgaccta cctcgccgcc cgccagcgca tcgacgacct ggtgcgcccg ctcgacgacg
     2821 acgacctctc ccgtcccgtg ccgacctgcc ccggctggac cgtgcacgcc gtcgtcagcc
     2881 acctcgcggc cacgccggat tgggccgcgg ccggtcggct cacggggttg ccctccgacg
     2941 aggacaccgc ggcgcaggtg gccgagctgg ccgacacccc gaccggcgag gtcctcgacc
     3001 gctgggcgac cactgctccc ggcttcgccg agctggccgc cggtgccgac atctggccgg
     3061 ccgcgatcga catcgtcacc cacgagcacg acatccgcca cgcactcggt cgccccgggg
     3121 agcgggacac ggaggccgtg ggcacgctgg ccgaggcgct cctggcctgg tggtcgccga
     3181 gccgggcggt ggaggtcgtc acacccgccc gcgccgtccg ctgcgggccg gcggaccagg
     3241 agccgctgcg ctggaccagc accgacttcg aggtcctgcg ggcccggctc ggacgccgca
     3301 gccgtgccca gctggcagcg atggactggt cggacgatcc cggcgagctg ctggacgagc
     3361 tgaccgtgtt cccgccgagc gggaccgaca tcgacgagtg accccgacga cgccggctcg
     3421 cctcagctga cctcgccgac caggagggcg gggttctccg aggagaccac gccgggcgcg
     3481 acctcctggg tgatggccag ggcgtcgagc gccgggtcgg cgcggaaggc cacgacctcc
     3541 ggtgaggggc cgagcacgcc gagggacacg accaggtcac cctggatgcc ccagagctgg
     3601 tacgtctcgg agtcgtccag cgcagggagc gtgttgccca tcaggtagcc ggcgccgtcc
     3661 cggtcgatgg cagcgggcgc ctccagctca ccgccgtccg gcgagcggag caccacgcgg
     3721 gtggcatcgg ggtcgttcat gacgtcgcgg gcgacgtcgg tcagtgcgat ctggacatcc
     3781 gggtcccgtt cggcgtcgcc gccgccgatg accaacccgg cgacgagggc gaccacgacc
     3841 agcgcagccg ccaccgcagc cccgggcagc cagcgcggca cgctgcggcg ctgccgtcgt
     3901 tcggcgaggt cgacgacgtc gtccggggcg gggccggggc gcgtcaggcg caggtccatc
     3961 tcggggggag cttcttcgag gctggccgag atccgggtcc acaggccctc gggggccggt
     4021 gcaccggagt gggcgagcat ggcggccacc tcgcggtggt cggccacctc ggcggcgcag
     4081 cgggggcaga cggcgaggtg ggcctcgacg gtggcggcct cgtcggcgtc cactgcatcg
     4141 agggcgtacg ccccgagcag ctcggcgatc tcgtcgtgtt cgttcatgct ccaccaccga
     4201 tcccgatgcc ggcgagctcg ccgcgcatct tcttcaaccc ggagcggatc cggctcttca
     4261 ccgtgccctc cggtgtcccg agcaggtcgg cgacctcccg gtacgtgtgc ccaccgaagt
     4321 atgcgagcaa gatggcctcc cgctcgccct ccggcagcga ttgcagcgtg accttcaccc
     4381 ggtcggccac cgccatgtcc cagacctcgt gctcgaggtc gtagccggcc tcagcggcga
     4441 gggtgagctc cttctcctcc cgacgccggc gggacgactc ggagcggatg aggtcgaccg
     4501 agcggccgtg gcactgggcg agcaggtagg accggagcga accgcggtcc gggtcgaagc
     4561 ggtcggggtt gttccacaac cgcaggaaga cctcttgcac gacctcctcg gccagcgtgc
     4621 ggtcggcgag caggcggcgg gccagcgcga agacggcacc ggcgtgtcgc cggtacacct
     4681 ccgcgagggc atcctcgtgg tagcggccga tggacacgac gagggcggcg tcactggcct
     4741 cttggagctg ctcgatcgtc atcgtcccct tcttcggagc gcgaccccct cccggatgat
     4801 cctgctcggc catcaggcca ccttccggtc gaagcgggac accagctcga cccggggggt
     4861 gtgcgggaac aggtcgacga ggacggtcga gcgtcgctcg aagccggcct cggcgaggag
     4921 cccggcgtct cgcccgaagg cggcggcgtc gcagctgatc agcacgaggc ggcgggcccc
     4981 cgtggccagg atcggcgcca gggcggcctt gccgagcccg gcgcggggcg ggtcggccac
     5041 gaccaggtcc gccggtgacg gacgccagcg gtcgaccgag gagcgcacga cggtggcgtc
     5101 gcggtcggcg aggttgtcct cggcgtcggc gaccgacgat cgagccgact cgacgagcac
     5161 ggtggacatc ccgtcgccca gcgtggaggc gaacagcccc acgcccccgt agaggtcggc
     5221 gagccgcccg tggccccagg cgtcgccacc cgcatcgcgc accgcggcca cgagggcctc
     5281 ggccccgtcg gtgcgggtct ggaagaacga gcgggcggag atccggaacc ggcgaccggc
     5341 gacctcgtcg tggaaccagg tgtggcgacc ctcggcgagc tcgtcctcgc cgaccacgat
     5401 cacatcgggc ggcagggcga ggtcgtcggg gatgcccggc gccaccacgg cgagccgctc
     5461 ccccgtcgat ccaccgatgc gcagcgtcac ctcgtcggca gcaccgaacc gtccgtcacg
     5521 gatgagctcg tcgagccccg ggtgggcgac catgcagctc gcgatgggga ccgcatcgtg
     5581 ggaccgggcg ccacggaacg ccggcccggc gggcgtcacc aggagacgca gcgaggtccg
     5641 gaacccgcgg tcgggcagcg gcggagcgag cgacaccgag ccctcctgcc ggccgagccg
     5701 gcgcaggctc tcctcgacca tccgctgctt ccagcgccgc tgggcgtcga ccgtcaggtg
     5761 ctgccacccg catccgccgc agccgtcggc cacgtggggg cacggcggca cggcgcggtc
     5821 gtcgctggcg gtccggatgg tgacggcgtc ggccttgagc aggcggcgct cctccttggt
     5881 gacgaccgcc gtcaggacgt cgccgggcac cccaccgcgc acgaacacga cccgaccatc
     5941 gtcggctcgg gcgatgccgt caccgcccac ggccacgtcg tcgacgcgga gctccaggtc
     6001 gtgcacttcc gcaggctact gaccccctcg gatgtccgga cggcaccaga tgctctgcga
     6061 agatggcggc catgagcgac cgccccggga ccgaccgccc catcctcatc gccccgtcgg
     6121 tcctgccggc ggacttctcc cggctgggag acgccgtgac cgccctcgac gaggccggcg
     6181 tcgaccgcat ccagtgggac gtcatggacg gccggttcgt ccccaacctc accttcgggc
     6241 ccgatgtgat cgcctcgtgc cgggaccgca ccgagctgct cttcgaagcc cacctgatgg
     6301 tcgaggaccc cgatccgctg ctgcacacct ggatcgacgc cggctgcggc ctggtcatcg
     6361 tccacgccga gacgtgccgc cacctccacc gcacgctcgg gcgcatcaag gacctcggtg
     6421 ccggggccgc ggtggcgctc aacccctcga ctccccccga ggtggtcgag cacgtcctcg
     6481 acctgtgcga catggtgctc gtgatgaccg tgaaccccgg gttcgggggg cagcgctaca
     6541 tcgagacgat ggagcccaag gtggccacca tccgccgctg gatcatcgac ggcggccacg
     6601 actgcgacat cgaggtcgac ggcggcatct cccccgagac ggtccgcggc ccggtggccg
     6661 ccggcgccaa cgtcgtcatc tcgggctcgt ccatgttcaa ggaccccgac ggcctggggc
     6721 acgcggtcga cgagctccgg cgcctggccg aggccgcccg ggcctgatgg cgcaccgact
     6781 cccggcggcg ttcctcgtcg ctgccctggc cgtggtcggc gcggcgtgca acgacgacga
     6841 cggcagcggg aacctggccg cgttctgcga ggccgccgcc gacacccagc ggttcgagac
     6901 ggtcttcgac gacctcgacc cgaccaacgt cgaggcggcg cgggagacgt tccgcaccgc
     6961 ccgggacgcc caggagggcc tccgcgagct cgcacccgag gcggcacgag cggacatcga
     7021 cgtggtgatc gcgttcgtcg acgacctcat cgtcgggctc cagcccgggc gcgacgtcga
     7081 cgagcacggc cgcccgagcg tctaccaggg gctgcggccc cgattcgacg aggtcgaggc
     7141 ggcgggcgac cgcctgcggg tgtacgtcga gtcgaactgc tgagctgccc ccgcccggga
     7201 ggcggctcag tcggtctcgc cgcggcgggt gatcaccttg tcgacgatgc cgtactccaa
     7261 ggcctgctcg gccgtgaacc agcggtcgcg ctcggagtcg gcctggatct gctccaccgt
     7321 ctgaccggtg tgctcggcga tgcgctcggc catcagctta ttgatgaagg cgagctgctc
     7381 ggcctggatg gcgatgtcgg aggcctggcc ctgcaccccg ccgagaggct ggtgcatcaa
     7441 gatgcgggcg ttgggcaagg tgtagcgctt gccctcggcc ccggcggtga ggaggaactg
     7501 gcccatggag gcggccagac ccatgcagat ggtggcgaca tcgggtgaga tgaactgcat
     7561 ggtgtcgtag atcgccatgc cggcggtgat gaccccaccg ggggagttga tgtacagcca
     7621 gatgtccttg tcgggatcct ggccttcgag gtacag
//
LOCUS       DATISU010000079        17600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_609229, whole genome shotgun sequence.
ACCESSION   DATISU010000079 DATISU010000000
VERSION     DATISU010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 17600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17600
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609229"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1551
                     /locus_tag="VLR27_03440"
     CDS             22..1551
                     /locus_tag="VLR27_03440"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSP02526.1"
                     /translation="MADPIALPPTGPGAPTPPIEISGGAADARAHLGAMVPVPDPVID
                     RLRKTGAGVSTDHAVRAEASRDWWPGAMIWATEGEVPGLAGVVVSPTDAEQVAAVLRL
                     CNAARVPVTAAGGRSGVCGASVPVFGGVVLDTTALTGVVSVDEESMILDVAPGTFGDH
                     LEAELRATGLTVGHWPQSVALSTVGGWVACRGAGQLSNKYGKIEDMVVGLDVVLADGT
                     AVTTGGGPRSAVGPDLTQVFVGSEGTLGVITGVRLRLHPAPKAQRHVAYGFDSFSAAN
                     EACRRIVQRGLPPAVLRVYDAIEADRSYQTGDRAVLLVMDEGDPIVVDAAMAITAEVC
                     ADAEPLDEGLVGRWLDHRNEVSALEALIGKGYVVDTMEISAPWSRLDDIYAAATEALR
                     SVPGTLAASAHQSHAYSDGACLYFTFAAKVDEDGEGTAARDRTYAQLWDVGQRAVLQA
                     GGTLSHHHGVGLNRSRFTAEALGAGHGVLQSIKDALDPNGILNPGKLGLRSPFGEVAY
                     P"
     gene            1548..1946
                     /locus_tag="VLR27_03445"
     CDS             1548..1946
                     /locus_tag="VLR27_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02527.1"
                     /translation="MIGGLLHPRAVAAGAGVALAICVPAALLAQLLDESDTVDDNSSW
                     LIVLFGVILVGMGIGGFVAANLRLDAPLTNSAVAALAAYLLVQTIGAVRLLASGDEVT
                     WAAIPFFALLSVSAGMAGGLLADRRARRPR"
     gene            1943..3373
                     /locus_tag="VLR27_03450"
     CDS             1943..3373
                     /locus_tag="VLR27_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HSP02528.1"
                     /translation="MTILVVDAGTSGVRSVALDADGTVVAEVLVEVLPSSPAPGLVEI
                     DAAATARAALATATSVLDQVGAVEAVGITDQRASTIAWDARSGDPVGPGIGWQDLRTV
                     GRCLALQPEGIRIAPNLSATKAEWLVQHSEIPAEHLRIGTVDTWLAWVLSDGAAHITD
                     ASNALVTGLYDPATRDWSDDLLGLLGIPRSALATVTDSSGSLAEATALPGAPPIAALV
                     GDQQASLLGQGCIRPGDTKLTLGTGGMLDVCIGPEPGPAPHHGTFPMIGWRLDGVDTW
                     AREAVMLSAGTCVEWLRDGLGIIDEVAESEALAGSVPDSDDVVFVPALSGLGTPAWDH
                     GARGLLVGITRGTTRAHIARAVLEGVALRAADLVDAVEADTGLRADVLRVDGGMSANR
                     VVLQALADATQQPVERSGHREATAIGAGFLAGVAVGTWSDLAAATATRTPTELIEPAG
                     TLDRERFADACRRGAGWIPELSSLEL"
     gene            3409..4035
                     /locus_tag="VLR27_03455"
     CDS             3409..4035
                     /locus_tag="VLR27_03455"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HSP02529.1"
                     /translation="MPTPRADRPDRRSFLRRAGAGGAVAIGTVLVPAVRFLPAAGAQA
                     SNAQDLAAFGESLELFAVDVYERGIELLSEDLAPLLQTFRGHHQEHAEAFAAVAGSAA
                     AGEANAALLEALNPAIEAFSTQNEVLRFARDLEDQLSVTCGHLLTLLDDPDVVATTAT
                     ILPIEAGHAVELSYELQEGPEVWFPSGSSERSDITFGYDPAVFPVGGA"
     gene            4035..4715
                     /locus_tag="VLR27_03460"
     CDS             4035..4715
                     /locus_tag="VLR27_03460"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HSP02530.1"
                     /translation="MASDDGTSRRDVLRFGGMAVTLGAVLAACSSDDEPSAPDPEAEA
                     DAEAEAEAERTEARQLADADVDVSLLNTALSLEVLAIDTYQAAFDFGLIQSAPVIDAA
                     TLFQQHHREHRDLLVGAVESAGAEPFLTPNPVVRAALVDPSLASISGEGDFLTLARDL
                     EQASAQLHVHATTSLSTQALRSTAMSIAGVAARRSKLLVLLGDLGSERLAFVPTDNPL
                     PSDAVVPG"
     gene            complement(4723..6033)
                     /locus_tag="VLR27_03465"
     CDS             complement(4723..6033)
                     /locus_tag="VLR27_03465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSP02531.1"
                     /translation="MTSQLVDVAVIGLGSAGEALSTALAEHGRHVVGFEPGRVGGECP
                     FTACMPSKALLHDAVSGRSWEDARRHRDEVVDHLDDGSHADALQQAGVALVRSAARLI
                     DEGVVEADGRRWRARHVVLATGSSPVVPPIAGIDDVPWLTSDELMVADELPESLLVLG
                     GGAIGCEAATILAGFGKQVTIVESSALLGGSVDPAVADRLAVQLRDTGVEVCTDAELA
                     EVSPASGGLRASLSTGATFDAATMLVATGQAPAWSELGLDQVGLDDEPDVDDEYRVGG
                     LDWLRAIGDLNGRSPWTHGANHEARRLARMLVDEPPGPPTEHMAHCVFTAPPVAAIGL
                     TAEAAHDEGRDVVVGTARYSDVARYSTDDLEDGIVVVVADRATGELLGCSGTGARFDD
                     VIGIVAALLHCGCTVQGAAELVLPFPTIGQVLTPAFRDAADARS"
     gene            6302..6646
                     /gene="rnpB"
                     /locus_tag="VLR27_03470"
     ncRNA           6302..6646
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VLR27_03470"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(6654..7586)
                     /locus_tag="VLR27_03475"
     CDS             complement(6654..7586)
                     /locus_tag="VLR27_03475"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HSP02532.1"
                     /translation="MSVVRQPAVPSLAAAWERFWFPAVPAARFEIFRRLLYGYLVLDV
                     LVTTAWVRDHGSADAGLYQPLLIGRLLPLPVPTDAVVTVTMWTVLVGAVLGITGRSPR
                     LIGAITACAYLQWMVIAFSYGKVNHDRFPLLVALFVAVTVGRTSIRDRTPSTAAGWAL
                     RMTMVATVAVYFLSVVAKVRYGGWLWANSATLTQALLRRATFLGRELRDLPHLAVLAQ
                     WAAVVFEVLSPLLLVRHRIRWVLWGTAIGFHVVTYAVLSIGFYPQLLCLLAFLPLERL
                     VPVPQGATAETFGGEPAACAPPAPIGAQATAARV"
     gene            complement(7583..8038)
                     /locus_tag="VLR27_03480"
     CDS             complement(7583..8038)
                     /locus_tag="VLR27_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02533.1"
                     /translation="MSARGRGWRSAVALVVGLLVVAGTVVGSDDWFPFAPMRQYAYAV
                     DPNGGVSTHFVEGIGVDGEPVRITYGDIDMRPAELEGILRPVETRTERLAEVLATVRA
                     RRPDAAPMVGIRYVRRRVQLVDRRPSPPELIELGRYEAPTGVVPEVPAP"
     gene            8200..10062
                     /locus_tag="VLR27_03485"
     CDS             8200..10062
                     /locus_tag="VLR27_03485"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HSP02534.1"
                     /translation="MVTAAAVAAVTIFVAVPAGAAAPEARSTAPACTFDGGFEDGFAD
                     VTSSNTHEASVDCIVYWQVTTGMRPGVYAPAGTVTRGQMASFLARFIEESGGSLPSNP
                     PDAFPDDDGTTHEANIDKLAAAKIAGGRADGTYAPDGAVFRNQMASFIVRTLEYRFGA
                     PIEDGVGADYFTDDDGDTHEGNIDKAAALGITGGTSDTTYGPQLRVRRDQMASFLARS
                     LAELVDTGHAEIPAPPPKCDLDDGIERTDSQCRPCPTDPSIAMSDPGCQTWSGAPPLG
                     DPALRQTPDAPQVWDNSDPSVLVDGQRVYLFGSTNNMRVPVREVDTTFQRSLQESKAD
                     WDRSPTNAMPQLPAWVDRGEADIWAPTVEKIGSRYVMYFAARHAGATTDENNDQCIGR
                     ATSSSPMGPYAAEAQPVYCGLPPEGAANGLPASNSFGRGALDPEVFRAPDGALYLLVA
                     ITRTEDNIGVLRLGADGSVQGGINATPTILQRVAYPWHDGTDDGARFGGFLENPSMVF
                     ERQTGTYLLFYSAGTWYTQDYNTGFARCATPMGPCTPDSRGPFLKTGPSRSGPGGFTA
                     FAAPDGSLRGVYATWQAGHEQDSDGIGTYSRQSHFARIVVTATHDPASQRVQLG"
     gene            complement(10131..11486)
                     /locus_tag="VLR27_03490"
     CDS             complement(10131..11486)
                     /locus_tag="VLR27_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HSP02535.1"
                     /translation="MVGRVLRVALLGVLAAAGVVAIAPAAGAELPPGGTFADDDGNVH
                     EGAIEAIVAEGITSGCVTNAKRYCPLSKVTRGQMATFLSRALALPAASRDHFDDDDGT
                     THEDAINRVAEAGITTGDASGSFRPGSPVSRGQMASFLVRALDLPAASTDHFADDDGT
                     THEDSINRVAEAGIASGGTDGRFRPGAAVQRDQMASFLSRALDLTPVVPPSRALAYGP
                     DAVSTMMDDLTNGGTIDSAWAVTTGIAGHYISYVTDLITVVGPYDATGWTEVSPATGA
                     SNLGGGRYRLDGLVLDWDRGDGLVDRVTDVIVRTDPGTGRVTVDDFWRANVPLSWIVR
                     HQGTASESNGVTARVRYNFQDVSFNQAPLIVTMEIENRNAYPIAVDGPSLHRDDGAFF
                     DADEFSAPIIDPGQKALAIAVFYELDLASHGGTVSMLTYGMTDDQEAMPSPRVHPWPR
                     R"
     gene            complement(11598..11978)
                     /locus_tag="VLR27_03495"
     CDS             complement(11598..11978)
                     /locus_tag="VLR27_03495"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSP02536.1"
                     /translation="MSIPVDLEALRDELAGHERPAYVITAGDTGGPHLVAVYLTWSDG
                     AFTAGCGRHTAANLAERPEVSVVVPPNEPDGYSLIFDATGEVVDGEQRTVRLTPVKAV
                     LHRPAAGPDDAGSCGHDCAPVGST"
     gene            12021..12383
                     /locus_tag="VLR27_03500"
     CDS             12021..12383
                     /locus_tag="VLR27_03500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HSP02537.1"
                     /translation="MGRIGARSKSAQAAVRARWRAWRVASHNRAMARECFYCGVAFAP
                     EGPDHRTVDHRVPRSQGGTESLRNLVFACYACNQRKRDRHEDDFLASEWIQRRRDDVE
                     ARAVPLARRRRLNGGRSG"
     gene            12393..13910
                     /locus_tag="VLR27_03505"
     CDS             12393..13910
                     /locus_tag="VLR27_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSP02538.1"
                     /translation="MSDHRSVWLATATHPTFDPLTADLDVDVAVVGGGITGLTTALLL
                     QRNGARVAVIEAGRVGGGTTGHTTGKVSSQHGLKYHQLIQDVGEDKARTYAEANQQAI
                     AKVRELADDVGADCDFETAPSFLFTTAPDEVEDLEAEHAAAVRLGLPASLTTETDLPF
                     RVQQALRFDDQAHFHAARYTAALARALDERGVQIVEQTRAMGVDEGKDGVTVKTERGD
                     VRAGHVVIATLLPFLDRGGFFAKARPKRAYGVAAVLRSGFLTGMHMTAGAPTRSTRPW
                     VDRDRRGIIVVGEDHETGSDEASPARYGALEDWARSHFDVESFEYRWSAQDFIPADGM
                     PYIGRSPRSSRTFVATGFQKWGLTNGTVAAQILADLIDGRDHPWLEAFDATRIGDPST
                     FTSLAKDNLKVGAHFVGDRIGRLRAGSIDHLAPGEGGIVKADGKTVGAYRAPDGTVHA
                     VSVDCTHLGCTVKWNSAEKSWDCPCHGSRFDCDGAVLDGPAVRPLSQVEVEINQT"
     gene            complement(13918..14901)
                     /locus_tag="VLR27_03510"
     CDS             complement(13918..14901)
                     /locus_tag="VLR27_03510"
                     /inference="COORDINATES: protein motif:HMM:NF045448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HSP02539.1"
                     /translation="MEARRAITSASRLRRLAEELVDEGVGFVAPDGVGDPLLEELDYA
                     LRPPVHEQRVPSYGAIIGPTVDADDWHISTQLQVSRRQTTGYRDAQIRRFADGFSAWA
                     VRVPHGLDEFVVFDRSAGSERDLVVLASASGSRLVQRDEAGIVRVVGHFGVVRSDMGG
                     WHHEPPLESWIEEIPGCLAAGQRTVLGHLLDFAVHDLGARHIGALLIMHPTGELSANH
                     QQRLPVPPELRIQRPYDLAPLRHGLAQSDGATVFDADGVLRRMGIHLVPSRDAEEHVR
                     PLGGTKHTSALRYSYDDPEAVVIAVSDDGPVTVMRHGLVVGRSPDDDAIHA"
     gene            14973..16232
                     /locus_tag="VLR27_03515"
     CDS             14973..16232
                     /locus_tag="VLR27_03515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HSP02540.1"
                     /translation="MEPAGTHAAPLAAVPDPEGKRVAVRVTPDALRHIRGGHPWVFDR
                     SITSTSHEAPPGALAVVFDDRRRFAAIGLWDPTSPLRIRILHAGDPTTVDEGFWATRL
                     AEAEERRRPLVEDGTTTGYRWVNGESDGLPGLIVDRYATTAVVKVYSAAWLPHLPALV
                     PLVAEGLDRVVLRLSRAVADGTTHGLADGQVLVGTAPDGPVEFLEHGLVFGADVVHGQ
                     KTGHFLDQRDNRALVGSLAKGKDVLDVFACTGGFSVHAAAGGARSVHTVDLSEPAIET
                     SVANMGRNRHRPEVAACEHTTEVGDAFRVLERHAAADRRWDVVVIDPPSFAPRQEAVG
                     RALKAYARLTTLGLAVTRRGGLLVQSSCSSRIDADTFHRQLRDVARRQGHGLEEVART
                     THPIDHPATFPEAWYLKTAFVRVDPRP"
     gene            complement(16266..17156)
                     /locus_tag="VLR27_03520"
     CDS             complement(16266..17156)
                     /locus_tag="VLR27_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HSP02541.1"
                     /translation="MERLSRVAIAVVALAVASACGGAGEGAVERPALVTTTVSEDPVE
                     DPSTTVTTTPLTTTTEPPSTAAPRPDWLGTRVLEPGPDGMVPAQPTPPELVDRRLETP
                     DHLPRPATQAFQAEVSEVPDDVLARSTWRAECPVPREELRYVLVTFWGFDGEVHTGEL
                     LLRADAVDAVVAGFAHLHELRFPIEEMRVVEPWELDAPPTGDGNNTTAFVCRPTRGSS
                     TWSEHAQGRAVDINPFHNPYVKGDLVLPELATAYVDRTDLRPGMLTVDDVAGFTGVGW
                     GWGGHWRSLKDHMHLSSNDR"
     gene            complement(17178..>17600)
                     /locus_tag="VLR27_03525"
     CDS             complement(17178..>17600)
                     /locus_tag="VLR27_03525"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSP02542.1"
                     /translation="TRATAAAWDAPVEVDDRWIELDYGELDGTPMRDVDADLWRRWRR
                     DVAFAPPGGESLAALGARVRAACDDLAEAARDGHVVVVTHVSPIKAAVAWAMGVGDEV
                     SWRMFVAPASVTRIAVGDRGPSLHGFNDVAHLADVGPG"
BASE COUNT         2527 a         6556 c         6218 g         2299 t
ORIGIN      
        1 gggctcgtac agtggcccgc cgtggccgac ccgatcgccc tgccccccac cggacccggc
       61 gcgccgacgc cgcccatcga gatcagcggc ggcgcggccg atgcacgcgc ccacctcggg
      121 gcgatggtcc cggtgcccga cccggtgatc gaccggctgc gcaagaccgg cgccggcgtg
      181 tcgaccgacc acgccgtgcg ggccgaggcg agccgcgact ggtggccggg ggcgatgatc
      241 tgggccaccg agggcgaggt ccccggtctc gccggcgtcg tggtctcccc caccgatgcc
      301 gaacaggtcg cggccgtgct gcgcctctgc aacgccgccc gggtgccggt gacggcggcc
      361 ggcggccggt cgggcgtgtg cggggcgtcg gtcccggtgt tcgggggcgt cgtgctcgac
      421 accaccgccc tcaccggtgt cgtctctgtc gacgaggagt cgatgatcct cgacgtcgcc
      481 cccggcacct tcggcgacca cctcgaagcg gagctgcggg cgacgggcct caccgtcggg
      541 cactggcccc agtcggtcgc gctgtccacc gtcggcggtt gggtcgcctg ccgcggtgcc
      601 ggccagctgt cgaacaagta cgggaagatc gaggacatgg tcgtcggcct cgacgtcgtc
      661 ctggctgacg gcacggccgt caccacgggc ggcgggcccc gctcggccgt cggtccggac
      721 ctgacccagg tcttcgtcgg ctccgagggc accctcggcg tcatcacggg cgtccgcctg
      781 cggctccacc cggcgccgaa ggcgcagcgc cacgtcgcct acggcttcga ctcgttctcc
      841 gccgccaacg aggcctgccg gcgcatcgtc cagcgggggc tgccgccggc cgtgctgcgg
      901 gtgtacgacg ccatcgaggc cgaccgcagc taccagaccg gcgaccgggc ggtgctgctc
      961 gtgatggacg agggcgaccc gatcgtggtg gacgcggcga tggcgatcac cgccgaggtg
     1021 tgcgccgacg ccgaaccgct cgacgagggg ttggtcgggc gctggctcga ccatcgcaac
     1081 gaggtctcgg ccctcgaggc tctgatcggc aagggctacg tggtcgacac catggagatc
     1141 tcggcgccgt ggtcgcgcct ggacgacatc tacgccgcag ccaccgaggc cctgcggtcc
     1201 gtgcccggca cgctggcggc cagcgcccac cagtcccacg cctactccga cggggcctgc
     1261 ctctacttca ccttcgcggc gaaggtcgac gaggacggcg agggcaccgc ggcgcgggat
     1321 cgcacctacg cccagctctg ggacgtcggg cagcgcgccg tgctccaggc cggggggacg
     1381 ctcagccacc accacggtgt cggcctcaac cgcagccgct tcaccgccga ggccctcggc
     1441 gccggccatg gggtgctcca gtcgatcaag gacgcgctcg acccgaacgg catcctcaac
     1501 cccggcaagc tgggcctgcg cagcccgttc ggcgaggtcg cctacccgtg atcggcgggc
     1561 tgctccaccc ccgggccgtc gccgccggcg ccggcgtggc actggcgatc tgcgtgccgg
     1621 cggcgctcct cgcccagctg ctggacgagt ccgacacggt ggacgacaac tcgtcgtggc
     1681 tcatcgtcct gttcggcgtc atcctcgtcg gcatgggcat cggcggcttc gtcgccgcca
     1741 acctgcgcct cgacgcaccc ctcacgaaca gcgcggtggc cgccctcgcc gcctacctgc
     1801 tggtccagac gatcggtgcc gtcaggctgc tcgcatccgg cgatgaggtc acctgggccg
     1861 ccatcccgtt cttcgccctc ctctcggtgt cggccggcat ggccggtggg ctgctcgccg
     1921 accgacgggc ccggaggcca cgatgacgat cctcgtggtc gatgccggca cgtccggggt
     1981 ccgctcggtc gcgctcgacg cagatgggac ggtcgtggcc gaggtcctcg tcgaggtcct
     2041 gccgagctca cccgcacccg gtctcgtgga gatcgacgcg gcagccaccg ctcgggccgc
     2101 cctggcgacg gccacgtcgg tgctcgacca ggtcggcgcg gtcgaggccg tgggcatcac
     2161 cgaccagcgg gcctcgacca tcgcctggga cgcccggtcg ggcgatccgg tgggccccgg
     2221 catcgggtgg caggacctgc gcaccgtcgg tcgctgcctc gccctgcaac ccgagggcat
     2281 ccgcatcgca ccgaacctgt cggccaccaa ggccgagtgg ctggtgcagc acagcgagat
     2341 cccggccgag cacctgcgca tcggcaccgt ggacacctgg ctggcctggg tgctgtccga
     2401 cggcgcggcc cacatcaccg acgcctccaa cgcgctggtg accggtctct acgacccggc
     2461 gacccgggac tggtccgacg acctcctcgg cctgctcggc atcccccgat cggcgctcgc
     2521 cacggtgacg gactccagcg ggtcgctggc ggaggcgacg gcgctgcccg gcgcgccgcc
     2581 gatcgccgcg ctcgtcggcg accagcaggc atcgctcctc ggccaggggt gcatccgccc
     2641 cggcgacacg aagctcaccc tcggcaccgg cgggatgctg gacgtctgca tcggacccga
     2701 gcccggcccc gccccccacc acgggacctt ccccatgatc ggctggcggc tcgacggtgt
     2761 cgacacctgg gcgcgcgagg ccgtgatgct gtcggccggc acctgcgtgg agtggctgcg
     2821 cgacggcctc gggatcatcg acgaggtcgc cgagagcgaa gcgctcgccg gctcggtgcc
     2881 ggactccgat gacgtggtgt tcgtccccgc cctgtccggg ctcgggacgc cggcctggga
     2941 ccacggcgcc cgcgggttgc tcgtgggcat cacccggggc accacgcgag cccacatcgc
     3001 ccgagcggtc ctggagggcg ttgccctgcg ggccgccgac ctggtggacg cggtcgaggc
     3061 cgacacgggc ctccgggccg acgtcctccg ggtcgacggg ggcatgagcg cgaaccgcgt
     3121 cgtcctgcag gcgctggccg acgcgaccca gcagccggtg gagcggtccg ggcaccggga
     3181 ggcgaccgcg atcggggccg gcttcctcgc cggcgtggcc gtcggcacgt ggtccgacct
     3241 ggcagcggcg acggcgacac ggacacccac cgagctgatc gaaccggccg ggacgctcga
     3301 ccgggagcgg ttcgccgacg cgtgccgccg tggcgcaggc tggatccccg agctctccag
     3361 cctcgagctc tgaccccacc caccaccccc gtcccaggag cccaccgcgt gccaaccccc
     3421 cgggccgatc gacccgaccg ccggtccttc ctccgccgtg ccggcgcggg cggcgcggtc
     3481 gcgatcggca ccgtgctcgt cccggcggtc cgcttcctgc ccgccgccgg agcccaggcc
     3541 agcaacgccc aggatctggc cgccttcggg gagtccttgg agctcttcgc cgtcgacgtc
     3601 tacgagcgcg gcatcgagct cctctccgag gatctcgccc ctctgctgca gacgttccgc
     3661 ggccaccacc aggagcacgc cgaggccttc gccgccgtgg ccgggtcggc ggctgcgggc
     3721 gaggcgaacg ccgccctgct cgaggccctg aacccggcga tcgaggcgtt ctcgacccag
     3781 aacgaggtgc tccgcttcgc acgcgacctg gaggaccagc tgagcgtcac ctgcggtcac
     3841 ctcctcaccc tcctggacga tcccgacgtc gtggcgacga ccgcgacgat cctgccgatc
     3901 gaggcgggcc acgccgtcga gctcagctac gagctccagg agggacccga ggtctggttc
     3961 ccctccgggt cctccgagcg ctcggacatc accttcggct acgacccggc cgtcttcccg
     4021 gtcggtggcg cgtgatggcc tcggacgacg gcacgtcgcg acgcgacgtc ctccggttcg
     4081 gcggcatggc cgtcaccctc ggcgccgtgc tcgccgcgtg cagctccgac gatgaaccgt
     4141 cggcgcccga cccggaggcc gaggcggacg cggaggcaga agcggaggcc gagcggacgg
     4201 aggcccgcca gctcgccgat gccgacgtgg acgtcagcct gctcaacacg gcgctgtccc
     4261 tggaggtgct cgccatcgac acctaccagg ccgcgttcga cttcggcctc atccagagcg
     4321 cccccgtcat cgacgccgcc accctcttcc agcagcacca ccgggagcac cgggacctcc
     4381 tcgtcggcgc ggtcgagtcg gccggcgcag agccgttcct cacccccaac ccggtggtga
     4441 gggcggcgct cgtcgatccg agcctggcgt cgatcagcgg cgaaggggac ttcctcaccc
     4501 tggcccgcga cctcgagcag gcgtccgccc agctccacgt gcacgcgacg acgtccctga
     4561 gcacgcaggc gctgcggtcg accgccatgt ccatcgccgg ggtggcggcc cgacgctcga
     4621 agctcctcgt cctgctcggc gacctcggct ccgagcgcct cgccttcgtg ccgaccgaca
     4681 acccgctgcc gtccgacgcc gtggtccccg gctgacccgg agtcagctcc tggcgtcggc
     4741 tgcgtctcgg aacgcggggg tgagcacctg gccgatggtc gggaagggca gcacgagctc
     4801 ggccgctccc tggacggtgc agccgcagtg caggagggcg gcgacgatgc cgatgacgtc
     4861 gtcgaagcgg gcgccggtgc cggagcagcc gaggagctcc ccggtggcgc ggtcggcgac
     4921 gacgaccacg atgccgtcct cgagatcgtc ggtggagtag cgggcgacgt cggagtagcg
     4981 cgccgtgccg accacgacgt cgcgcccctc gtcgtgggcg gcctccgcgg tgagcccgat
     5041 ggccgcgacc ggcggggccg tgaacacgca gtgcgccatg tgctcggtgg gcgggccggg
     5101 cggttcgtcg accagcatcc gggcgagtcg gcgagcctcg tggttggcgc cgtgggtcca
     5161 gggcgagcgc ccgttcaggt cgccgatggc acggagccag tcgagacccc ccacccggta
     5221 ctcgtcgtcg acgtcgggct cgtcgtcgag acccacctgg tccaacccga gctcggacca
     5281 cgcgggcgcc tgcccggtgg ccacgagcat ggtcgctgcg tcaaaggtcg cgccggtgga
     5341 gagcgaggcc cggagccccc cggaggccgg cgacacctct gccagctcgg cgtccgtgca
     5401 gacctccacg ccggtgtcgc ggagctgcac ggcgagccgg tcggcgaccg cgggatcgac
     5461 gctgccgccg agcagggcgc tgctctcgac gatcgtcacc tgcttgccga acccggccag
     5521 gatcgtggcc gcttcgcagc cgatcgcacc gccgccgagg accagcagcg attccggcag
     5581 ctcgtcggcc accatcagct cgtcgctggt gagccacggg acgtcgtcga tgccggcgat
     5641 cggcggtacg acgggagagg agccggtggc gagcaccacg tggcgggccc gccagcgccg
     5701 cccgtcggcc tcgaccacgc cctcatcgat gagccgggct gcgctccgga cgagcgcgac
     5761 gcccgcctgc tgcagcgcgt cggcgtgcga gccgtcgtcg aggtgatcga ccacctcgtc
     5821 gcgatgtcgc cgtgcgtcct cccacgatct gccggagacg gcgtcgtgga ggagggcctt
     5881 gctgggcatg caggcggtga aggggcactc ccctccgacc cgtcctggct cgaacccgac
     5941 gacgtgtcgg ccgtgctcgg ccagcgccgt ggacagggct tctccggcgg agccgaggcc
     6001 gatgacggcc acgtcgacga gctgggaggt caccggttca cggcctgatc ctcgacgcga
     6061 gggcgaggct cacgccgacg aaggcggcgg tggcgtgcac gaggtggcga gcaggtcggc
     6121 ggatggcgcc ggggggtggg ggcagcgcga gcacgggaga cttcctggga cggatggtgc
     6181 gacggggaac ccggcgtccc tacccctcct gacccacgcc atgaccgaag tggccgacgc
     6241 cacatccgcg cgcgcgtggt gcgggatcgc cggtccggtg ccacactgag ccacgcgatg
     6301 gtgagtcgga ccggtgaccg cggccacccc cgggtggctg aggaaggtcg gggctccaca
     6361 gagcagagcg ctggcgagag ccaggacggg gcgacctgtc ggtacagggc aacagagaga
     6421 agaccgccga tggccgggtc cgcccggcac aggtgagggt gaaacggtgc ggtaagagcg
     6481 caccagcgtc gggggcgacc ccggcggctc ggcaaccccc gctcggagca aggccaagcg
     6541 tgccgcgggt tgctcgcccg atcggccagg tgggccgcac agatggatgg tcacccacgg
     6601 ggcgtcagcc ctgggacaga accccgccta caagccggct caccatcgcc ccatcaaacc
     6661 cgggccgccg tggcctgggc gccgatcggc gccggaggtg cgcatgctgc cggttcgccg
     6721 ccgaacgtct ccgcagtggc gccctggggt accggcacca gccgctcgag cggcaggaag
     6781 gcgagcaggc agagcagctg cgggtagaac ccgatggaga gaaccgcgta ggtcacgacg
     6841 tggaagccga tcgccgtgcc ccagagcacc cagcggatcc ggtgtcgcac cagcagcaag
     6901 ggcgacagga cctcgaacac gaccgccgcc cactgggcga ggacggcaag gtgcggcagg
     6961 tcccgcagct cgcgtccgag gaacgtcgct cgacggagca gcgcctgcgt gagcgtggcg
     7021 gagttggccc acagccaccc gccgtagcgg accttggcca ccacagacag gaagtacacc
     7081 gcgaccgtgg ccaccatcgt catccgcagg gcccacccgg ccgcggtcga cggcgtgcgg
     7141 tcccggatgc tggtccgtcc cacggtgacg gcgacgaaga gggccacgag cagcgggaat
     7201 cggtcgtggt tgaccttgcc gtacgagaac gcgatcacca tccactggag gtacgcgcac
     7261 gcggtgatcg cgccgatcag gcgtggtgac cgcccggtga tgccgagcac cgcaccgacg
     7321 agcacggtcc acatcgtcac cgtgacgacg gcatcggtcg gcaccgggag cgggagcagc
     7381 cggccgatca gaagcggctg gtagagaccg gcatcggccg agccgtggtc ccgcacccac
     7441 gccgtggtga ccagcacgtc cagcacgagg tagccgtaga gcaaccggcg gaagatctcg
     7501 aagcgggccg ccgggaccgc ggggaaccag aagcgctccc acgctgcggc gagcgagggc
     7561 acggccggct gtcggacgac gctcacggtg ccggcacctc gggaacgacc cccgtcgggg
     7621 cctcgtagcg gcccagctcg atcagctcgg gcggactcgg tctgcggtcc acgagctgca
     7681 cgcgccggcg cacgtagcgg atcccgacca tcggcgccgc gtcaggccgc cgcgcccgga
     7741 cggtcgccag cacctcggcc aggcgctcgg tgcgcgtctc gaccggccgc aggatgccct
     7801 cgagctcggc cggtcgcatg tcgatgtcgc cgtaggtgat ccggaccggt tcaccgtcga
     7861 cgccgatccc ctcgacgaag tgggtggaca ccccgccgtt cggatccacg gcgtaggcgt
     7921 actgccgcat cggcgcgaac gggaaccagt cgtccgaccc gaccaccgtg ccggccacca
     7981 ccaggagccc gacgaccagc gcgacagctg accgccagcc ccgcccacgc gctgacatcg
     8041 caggagatcc tacgcccgat ggcacctcgc cgacgggggc cggaggtgcc gcgaccgttc
     8101 cctcggccac ggagcgggtg catgcgaaca cgggttgtag ggttgccggc cgacgcaaga
     8161 cgacgagcaa ctggggagcc gatgcgccgg ttcgtgagca tggtgaccgc agctgcggtc
     8221 gccgcagtga ccatcttcgt ggccgtgccg gcaggcgcgg cggcacccga ggccaggtcg
     8281 acggcgccgg cgtgcacctt cgacggcggc ttcgaggacg gcttcgccga cgtcacgagc
     8341 agcaacaccc acgaggccag cgtggactgc atcgtctact ggcaggtcac gacggggatg
     8401 cgccccggcg tctacgcacc ggccgggacg gtgacgcgcg ggcagatggc gagcttcctg
     8461 gcccggttca tcgaggaatc cggtggctcg ctgccgtcga acccgccgga cgccttcccc
     8521 gacgacgacg gcaccaccca cgaggccaac atcgacaagc tcgccgcggc gaagatcgcc
     8581 ggcggtcgcg ccgacggcac ctacgcgccc gacggagccg tgttccgcaa ccagatggcg
     8641 tcgttcatcg tgcggacgct cgagtaccgc ttcggtgcgc ccatcgagga cggcgtcggc
     8701 gccgactact tcaccgatga cgacggcgac acccacgagg gcaacatcga caaggcggcg
     8761 gcgctcggca tcaccggcgg cacctccgac accacctacg gcccgcagct ccgcgtccgt
     8821 cgggaccaga tggcgtcgtt cctggcccgg tcgctggccg agctggtcga caccggccac
     8881 gccgagatcc ccgcaccgcc gcccaagtgc gacctcgacg acggcatcga gcggaccgac
     8941 tcgcagtgcc gcccgtgccc gaccgacccg agcatcgcca tgtcagaccc gggatgccag
     9001 acctggtccg gcgcgccgcc cctcggcgat cccgcactcc gccagacccc ggacgcaccg
     9061 caggtgtggg acaactctga cccgagcgtg ctcgtggacg gccagcgcgt gtacctcttc
     9121 ggctcgacga acaacatgcg ggtgccggtc cgggaggtcg acacgacgtt ccagcggtcg
     9181 ctgcaggaga gcaaggccga ctgggaccgc tcgccgacca acgcgatgcc ccagctcccg
     9241 gcatgggtcg accgcggcga ggccgacatc tgggccccca cggtcgagaa gatcggcagc
     9301 cggtacgtga tgtacttcgc cgctcgccac gccggcgcga ccaccgacga gaacaacgac
     9361 cagtgcatcg gccgggcgac gtcgtccagc ccgatggggc cgtacgccgc tgaggcgcag
     9421 ccggtgtact gcggtctgcc gcccgagggg gccgccaacg gcctgccggc ctcgaacagc
     9481 ttcggtcggg gtgcgctcga tccggaggtc ttccgggcgc cggacggggc gctgtacctg
     9541 ctggtggcga tcacccggac cgaggacaac atcggggtcc tccgcctcgg tgccgacggc
     9601 tcggtgcagg gcggcatcaa cgcgaccccg acgatcctgc agcgggtcgc ctacccgtgg
     9661 cacgacggca ccgacgacgg cgcacgcttc ggcggcttcc tcgagaaccc gtcgatggtc
     9721 ttcgagcgcc agaccggcac gtacctgctc ttctactccg ccggcacctg gtacacgcag
     9781 gactacaaca ccggcttcgc ccgttgcgcc acaccgatgg gaccctgcac gcccgactcc
     9841 cgcggcccgt tcctgaagac cgggccgagc cgctccggcc ccggcgggtt caccgccttc
     9901 gccgcccccg atgggagcct gcggggggtc tacgccacct ggcaggccgg ccacgagcag
     9961 gacagcgacg ggatcggcac gtacagccgc cagtcgcact ttgcccggat cgtggtcacg
    10021 gccacgcacg accccgccag ccagcgggtg cagctcgggt gatcagccgc catcagcgtc
    10081 ggcgacgaga accaccggac atccctggtc acggatggcc ggtctgaggc tcaacgcctc
    10141 ggccaagggt gcacacgggg ggacggcatg gcctcctggt cgtccgtcat gccataggtg
    10201 agcattgaca ccgtcccgcc atgacttgcg agatcgagct cgtagaacac ggcgatcgcg
    10261 agagccttct gtccaggatc gatgatcggt gcagagaact cgtcggcgtc gaagaacgct
    10321 ccgtcgtccc ggtggagcga cggaccgtcc accgcgatcg ggtaggcgtt ccggttctcg
    10381 atctccatcg tcacgatgag cggcgcctgg ttgaaggaga cgtcctggaa gttgtagcga
    10441 acgcgggctg tcaccccgtt cgactccgaa gctgtgcctt ggtggcgcac gatccagctc
    10501 aacgggacgt tcgcacgcca gaagtcatca accgtgacgc gcccggtgcc cgggtcagtg
    10561 cgaacgatca cgtcggtcac ccggtcgacc agcccgtcgc cgcgatccca gtccagcacg
    10621 agaccatcca gtcggtaccg cccgccaccg aggttcgacg caccggtggc gggactgacc
    10681 tcggtccagc cggtcgcgtc gtaggggcca acaacggtga tcaggtccgt gacgtagctg
    10741 atgtagtgac cagcgatccc agtcgtcacc gcccacgcgc tgtcgatggt ccccccgttt
    10801 gtgagatcgt ccatcatcgt cgacaccgca tcgggtccgt aggcaagggc acgggagggt
    10861 ggcaccaccg gtgtcaggtc gagcgccctg gataggaagg acgccatctg atccctctgg
    10921 acagcagccc cgggccggaa ccgaccgtct gtcccaccac tcgcgattcc cgcctcggcg
    10981 acccggttga tcgaatcttc gtgcgtcgtg ccgtcgtcgt cggcgaagtg gtcggtggag
    11041 gccgccggga gatccagagc gcggaccagg aacgatgcca tctggcctcg tgagaccggt
    11101 gagccgggcc tgaacgaccc cgacgcgtcc cccgtcgtga tccccgcctc agccacccgg
    11161 ttgatggcgt cctcgtgcgt ggtcccgtcg tcgtcgtcga agtgatctcg agacgctgcg
    11221 ggcaacgcca aggcacgact gaggaacgtc gccatctgcc ctctggtgac cttgctgagc
    11281 gggcagtagc gcttggcgtt cgtgacgcac ccagaggtga tcccctcggc gacgatcgcc
    11341 tcgatggccc cctcgtgcac gttgccgtcg tcgtctgcga acgtgccgcc aggcggcagt
    11401 tcggcgccag ctgccggcgc gatcgcaacg acgccggccg ctgcaaggac ccccagcagt
    11461 gcaacgcgaa gtactcgacc caccatgtcc acccttcatt ccgccgaacg agacgtgggc
    11521 ccgcgcccac ggtggacgag tatgtcacca cccatcccgc atgcagagga tcggacggac
    11581 cagatcccgt tgcgcggtca ggtcgaaccg accggagcgc agtcgtggcc gcagctgccg
    11641 gcatcgtccg gtccggcggc gggccggtgc agcacggcct tgaccggggt caagcgcacg
    11701 gtgcgctgct ccccatccac cacctcgccg gtggcgtcga agatcagcga gtagccgtcg
    11761 ggctcgttcg ggggcacgac cacgctgacc tcgggccgct cggccaggtt ggctgcggtg
    11821 tgccggccac acccggcggt gaaggcgccg tcggaccagg tgaggtagac ggccacgagg
    11881 tgtggcccgc cggtgtcacc ggcggtgatc acgtaggcgg gccgctcgtg gcccgccagc
    11941 tcgtcgcgca gtgcttcgag gtccacgggg atcgacatgg tccgaggtta cggcggcacg
    12001 gcggcgcgta gcgtcaggac atgggacgga tcggggcgcg cagcaagtca gcgcaggcgg
    12061 cggtccgggc ccgttggcgc gcctggcggg tcgcctcgca caaccgggcg atggcgcggg
    12121 agtgcttcta ctgcggcgtc gcgttcgcgc cggagggtcc cgaccaccgc accgtcgacc
    12181 accgggtccc gcggagccag ggcggcaccg agtcgctccg caacctggtc ttcgcctgct
    12241 acgcgtgcaa ccagcgcaag cgggaccggc acgaggacga cttcctcgcc tccgagtgga
    12301 tccaacgacg ccgcgacgac gtcgaggccc gggccgtgcc gctggcccgc cggcggcggc
    12361 tgaacggcgg ccggagcggg tagggcgcgt ccatgtccga ccaccgctcc gtctggttgg
    12421 ccacggccac ccaccccacc ttcgacccgc tcaccgccga cctcgacgtg gatgtcgccg
    12481 tcgtcggtgg cggcatcacc gggttgacca ccgccctgct cctgcaacgc aacggcgccc
    12541 gcgtcgccgt gatcgaggcc gggcgcgtcg gcggcggtac caccgggcac acgaccggca
    12601 aggtgagctc ccagcacggg ctcaagtacc accagctgat ccaggacgtg ggcgaggaca
    12661 aggcccgcac ctacgccgag gccaaccagc aggccatcgc caaggtccgt gagctcgccg
    12721 acgacgtcgg cgccgactgc gacttcgaga cggctccctc cttcctgttc accaccgcgc
    12781 ccgacgaggt cgaggacctc gaggccgagc acgccgccgc ggtacgcctc gggctgccgg
    12841 cgtcgctcac caccgagacc gacctcccct tccgcgtcca gcaagccctg cgcttcgacg
    12901 accaggccca cttccacgcc gcccgctaca ccgcggcgct cgcccgcgcc ctcgacgagc
    12961 gcggcgtgca gatcgtggag cagacccggg ccatgggcgt cgacgagggc aaggacgggg
    13021 tcacggtcaa gaccgagcgc ggcgacgtgc gggccggcca cgtcgtgatc gccacgctgc
    13081 tcccgttcct cgaccggggc ggcttcttcg ccaaggcccg gccgaagcgg gcctacggcg
    13141 tcgccgccgt cctgcgcagc gggttcctca ccggcatgca catgacggcc ggcgcgccca
    13201 ctcgctccac ccgtccctgg gtcgaccggg accggcgggg catcatcgtc gtcggcgagg
    13261 accacgagac cggcagcgac gaggcctcac cggcccgcta cggcgccctc gaggactggg
    13321 cccggtcgca cttcgacgtc gagtccttcg agtaccgctg gtcggcgcag gacttcatcc
    13381 cggccgacgg catgccctac atcggccgct cgccccgcag ctcgcgcacc ttcgtcgcca
    13441 ccggcttcca gaagtggggg ctcaccaacg gcaccgtcgc cgcccagatc ctcgccgacc
    13501 tcatcgacgg tcgcgaccac ccctggctcg aagccttcga cgccacccgg atcggcgacc
    13561 cgtccacgtt cacgagcctg gccaaggaca acctcaaggt cggcgcccac ttcgtgggtg
    13621 atcgcatcgg tcgcctgcgg gccggatcca tcgaccacct cgcgccgggc gagggcggca
    13681 tcgtcaaggc cgacggcaag acggtcggcg cgtaccgggc cccggacggc accgtccacg
    13741 cggtgtcggt cgactgcacg cacctcggtt gcaccgtcaa gtggaacagc gccgagaaga
    13801 gctgggactg cccctgccac ggctcccgct tcgactgcga cggtgcggtg ctcgacggcc
    13861 cggccgtgcg ccccctcagc caagtcgagg tcgagatcaa ccagacctga tggctggtca
    13921 ggcgtggatg gcgtcgtcgt cgggtgagcg gcccacgacc agcccgtggc gcatgacggt
    13981 gaccggaccg tcgtcgctga cggcgatgac caccgcctcg gggtcgtcgt agctgtagcg
    14041 cagcgccgaa gtgtgcttcg tgccgccgag cggacggacg tgctcctcgg cgtcgcggct
    14101 gggcaccagg tggatcccca tgcggcggag cacgccgtcg gcgtcgaaca ccgtcgcgcc
    14161 gtcggactgg gccaggccgt ggcggagcgg ggccaggtcg tagggacgtt ggatccgcag
    14221 ctccggcggc accggcaacc gctgctggtg gttcgccgac agctccccgg tcgggtgcat
    14281 gatcaacagc gcgccgatgt ggcgggcacc gaggtcgtgg acggcgaagt ccagcaggtg
    14341 accgagcacc gtgcgctgcc cggcggcgag gcaccccggg atctcctcga tccaggactc
    14401 cagcggcggc tcgtggtgcc agccgcccat gtcggagcgg acgacgccga agtggccgac
    14461 cacccgcacg atcccggcct cgtcccgctg gaccagccgg gaccccgagg ccgacgccag
    14521 caccacgaga tcccgctcgg agccggcgga gcggtcgaag accacgaact cgtcgaggcc
    14581 gtgggggacc cgaacggccc aggccgagaa gccgtcggcg aagcggcgga tctgcgcgtc
    14641 ccggtacccg gtggtctggc gccgggagac ctgcagctgg gtggagatgt gccagtcgtc
    14701 ggcgtcgacc gtggggccga tgatggcgcc gtagctcggc acccgctgct cgtgcacggg
    14761 cgggcgcagg gcgtagtcca gctcctcgag gagcggatcc ccgacgccgt ccggtgcgac
    14821 gaacccgacg ccctcgtcga cgagctcttc tgcgagacgg cggaggcgcg acgcgctggt
    14881 gatggctcgg cgggcttcca cggcgccacg ttacgggggg tcgggcccga tcgggcaggt
    14941 accgtcgacc gatcaccgac ccgggagcgg acatggagcc agccggcacg cacgcagcac
    15001 ccctcgccgc cgtccccgac cccgagggca agcgggtggc ggtgcgcgtc acccctgacg
    15061 ccctccgcca catccggggc gggcacccgt gggtgttcga ccgctccatc acctccacca
    15121 gccacgaggc gccgcccggt gccctggccg tcgtgttcga cgaccgccgc cgcttcgcgg
    15181 ccatcggtct gtgggacccg acctcgccgc tgcggatccg catcctccac gccggcgacc
    15241 ccacgaccgt cgacgagggg ttctgggcga cgcgcctcgc cgaggccgag gagcggcgac
    15301 gcccgctggt ggaggacggg accaccaccg gctaccgctg ggtcaacggg gagagcgacg
    15361 ggctgccggg tctcatcgtg gaccgctacg cgacgaccgc ggtcgtgaag gtgtacagcg
    15421 cggcctggct cccccacctc cccgccctgg tgccgctggt ggccgaaggc ctcgaccggg
    15481 tcgtcctgcg cctgtcacgg gcggtcgcgg acggcaccac ccacggcctc gccgacggac
    15541 aggtcctcgt cggcaccgcg ccggacggtc cggtggagtt cctcgagcac ggcctggtct
    15601 tcggcgccga cgtcgtccac ggccagaaga ccggccactt cctggaccag cgggacaacc
    15661 gtgccctggt cggctcgctg gccaagggga aggacgtgct cgatgtcttc gcctgcaccg
    15721 ggggcttcag cgtccacgca gcagcgggcg gcgcccgctc cgtgcacacc gtcgacctca
    15781 gcgagcccgc catcgagacg tcggttgcca acatgggccg caaccgccac cggccggagg
    15841 tggcggcgtg cgagcacacg accgaggtcg gcgacgcgtt ccgggtcctc gagcgccacg
    15901 ccgcggccga ccgccgctgg gacgtcgtgg tgatcgaccc gccgtcgttc gcgccgcgtc
    15961 aggaagccgt cggccgggca ctcaaggcct acgccaggct caccaccctc gggctcgccg
    16021 tcacccgtcg aggcggcctg ctggtgcagt cgtcctgctc cagccggatc gacgccgaca
    16081 cgttccaccg ccagctgcgc gacgtcgccc gccggcaggg ccacgggttg gaggaggtgg
    16141 cccggacgac ccacccgatc gaccacccgg ccaccttccc tgaggcctgg tacctgaaga
    16201 cggcgttcgt gcgggtcgac ccacggccct gaccgaccgg ccgctacacc tgcactgcca
    16261 gaacgtcagc ggtcgttgga ggagaggtgc atgtggtcct tcagtgagcg ccagtggccg
    16321 ccccagcccc agccgacgcc ggtgaacccg gcgacgtcat ccacggtgag catgccgggg
    16381 cgcaggtcgg tgcggtccac gtatgcggtg gcgagctcgg gcagcaccag gtcgcccttc
    16441 acgtaggggt tgtggaaggg gttgatgtcg accgcccggc cctgggcgtg ctcggaccac
    16501 gtgctcgagc cccgggtggg ccggcagacg aaggcggtgg tgttgttccc gtcgccggtg
    16561 ggcggtgcgt cgagctccca cggctccacg acgcgcatct cctcgatcgg gaaccggagc
    16621 tcgtgcaggt gggcgaaccc cgccaccacg gcatcgacgg cgtcggcccg caggagcagc
    16681 tcgccggtgt ggacctcgcc gtcgaagccc cagaacgtca ccagcacgta gcgcagctcc
    16741 tcccgcggca ccgggcactc ggcgcgccag gtcgagcggg cgagcacgtc gtcgggcacc
    16801 tcggagacct cggcctggaa cgcctgcgtg gcgggtcgcg gcaggtgatc gggcgtctcg
    16861 aggcggcggt cgaccagctc gggcggggtg ggctgggccg ggaccatgcc atcgggcccc
    16921 ggctccagca cgcgggtgcc gagccagtcc ggccggggtg ccgcggtcga tggcggttcg
    16981 gtcgtggtcg tcagcggcgt ggtcgtcacg gtggtcgagg ggtcctccac cgggtcctcc
    17041 gacacggtcg tggtcaccag cgcgggacgt tcgaccgcgc cctcgcccgc cccgccgcac
    17101 gccgacgcca cggcgagcgc caccacggcg atcgctaccc ggctcagtcg ctccacctgg
    17161 tcgaccgtac cgacggctca gccgggtccc acgtcggcga ggtgcgccac gtcgttgaag
    17221 ccgtgcagcg acgggccccg gtcgccgacc gcgatgcggg tcaccgacgc cggcgccacg
    17281 aacatgcgcc aggagacctc atccccgacg cccatggccc aggcgaccgc cgccttgatc
    17341 ggtgagacgt gggtgaccac cacgacgtgg ccgtcacgcg ccgcctcggc caggtcgtca
    17401 cacgcagcgc ggacgcgcgc cccgagcgca gcgaggctct cgccaccggg cggcgcgaag
    17461 gcgacgtcgc gccgccaccg ccgccacagg tcggcgtcca catcccgcat cggggtgccg
    17521 tcgagctcgc cgtagtcgag ctcgatccac cggtcgtcga cctccaccgg tgcgtcccac
    17581 gctgcggccg tggcgcgggt
//
LOCUS       DATISU010000080         4564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_236494, whole genome shotgun sequence.
ACCESSION   DATISU010000080 DATISU010000000
VERSION     DATISU010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236494"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="VLR27_03530"
     CDS             complement(<1..1245)
                     /locus_tag="VLR27_03530"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798449.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HSP02543.1"
                     /translation="MAIAPERTGIGIRRFFTRPGAHPYDELEWERRDARLTNYQDGSV
                     AFEQLGVEFPRTWSLNATNIVTQKYFRGTPGTPEREDSLKQVVDRVCDTITDWGIEGG
                     YFVDDDEAEAFRSELKYLMVTQRAAFNSPVWFNIGVPGVPQQASACFILKVDDEMDSI
                     LNWYVEEGTIFKGGSGAGINLSKIRSSKELLKGGGTASGPVSFMRGADASAGTIKSGG
                     KTRRAAKMVILDVDHPDVEEFVWVKAREERKIRVLQENGFDMSLDGADMASVQYQNAN
                     NSVRVTDEFMQAVVDDADWNLTAVTTGEVVRTVRARDLMRQISQAAWECADPGMQFDT
                     TINRWHTCANTDRINGSNPCSEYMHIDNSACNLASINLLKFLDLDAGDRFDIEAFAHT
                     VDVVFTAQEILVGRADYPTEAIG"
     gene            complement(1734..4052)
                     /locus_tag="VLR27_03535"
     CDS             complement(1734..4052)
                     /locus_tag="VLR27_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HSP02544.1"
                     /translation="MTTTAPNPSAAPADADDAVTVQRTCPLCEASCGLDVTVRDGQVV
                     RIRGDRLDVFSHGYLCPKGSTLKHLHEDPDRLRQPLVRRGDRLEPATWAEAWQAVADG
                     LGRVIDEHGRSAVGAYVGNPNAHNLGALLYNRAFLRALGSPNVFSASTVDQRPKEISS
                     GLIFGNVLTNPVPDIDRTDHLLILGANPFASNGSFATAPDWPGRLRAILERGGRVVVV
                     DPRRTKTAEEASAHVPIRPGADPFLLAAMLQELFAADRVDLGTVAEHVARVDELQAAV
                     ALFTPEAVAGRCGIDAEAIRRLAHELADAPKAVVYARIGTTVSPYGTVTSWLVDALNI
                     CTGNLDRPGGAMFTKAAAGAANTRGPGRYGRGIRLGRRTSRVRGLPETFGELPVVALA
                     EEIETPGEGQIKAMITVAGNPILSTPDSVRLDAAFASLDFVVAIDIYLNETTRHADVI
                     LPAPSALQKSHYDVALLQLALRNVANYSPPVLPLDEGAPDEWEILCTLSLILQGVDPA
                     EADPALVDDAAIRSMVESAVADEHGLVHGRDVEELLEALAPRVGPERTLDLMLRTGPY
                     GDGFGARPDGLSLDVLLDHPHGVDLGPLQPRMPEALRQPDGMIDLAPEVLLADVATRM
                     VPALDEVDDGLLLIGRRHLRSNNSWMHNVEVLVKGKPRCTLQVHPDDAAERGLVDGGQ
                     AEVRSRVGAVVAPVEVTDAVMAGVVSLPHGWGHDLDGARLSVAAEHAGVNSNVLADTD
                     LFDPVSGTAVLNGIPVEVVAVEPAEAALVTHR"
     gene            4122..>4564
                     /locus_tag="VLR27_03540"
     CDS             4122..>4564
                     /locus_tag="VLR27_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HSP02545.1"
                     /translation="MRQLLPVPGDVDPIAAYLAAPRPAPAARPWVALSMVTSLDGATA
                     VGGRSGPLGGEPDRAVFRAVRAIADVVLVAAGTARTERYGPVRLSAPTAAARAEAGRS
                     ADPPRLVVVSGSLDLDLSRAAEGPVRPLVLTTEDADPTRRAALEEL"
BASE COUNT          627 a         1508 c         1714 g          715 t
ORIGIN      
        1 gccgatggcc tcggtcgggt agtcggcccg gccgaccagg atctcctggg cggtgaagac
       61 gacgtcgacg gtgtgggcga aggcctcgat gtcgaagcga tcgccggcgt cgaggtcgag
      121 gaacttcagc aggttgatcg aggcgaggtt gcaggccgag ttgtcgatgt gcatgtactc
      181 cgagcagggg ttcgacccgt tgatccggtc ggtgttggca caggtgtgcc accggttgat
      241 cgtggtgtcg aactgcatgc cgggatcggc gcactcccag gcggcctggg agatctggcg
      301 catcaggtcg cgtgcccgca cggtgcggac gacctcgccg gtggtgacgg cggtgaggtt
      361 ccagtcggcg tcgtcgacga cggcctgcat gaactcatcg gtgacccgca ccgagttgtt
      421 ggcgttctgg tactggaccg aggccatgtc ggcgccgtcc aggctcatgt cgaagccgtt
      481 ctcctggagg acgcggatct tgcgctcctc ccgggccttg acccacacga actcctccac
      541 gtcggggtgg tcgacgtcga ggatgaccat cttggcggcg cggcgggtct tgccaccgga
      601 cttgatggtg ccggccgagg cgtcggcgcc ccgcatgaag ctgaccgggc cggaggcggt
      661 gccgccgccc ttgagcagct ccttggacga gcggatcttg gacaggttga tgccggcgcc
      721 cgagccgccc ttgaagatcg tgccctcctc gacgtaccag ttgaggatcg agtccatctc
      781 gtcgtcgacc ttgaggatga agcaggcgct ggcctgctgg ggcaccccgg ggacgccgat
      841 gttgaaccag accgggctgt tgaacgcggc ccgctgggtg accatcaggt acttgagctc
      901 gctgcggaag gcctcggcct cgtcgtcgtc gacgaagtag ccgccctcga tgccccagtc
      961 ggtgatggtg tcgcacaccc ggtcgacgac ctgcttgagg ctgtcctcgc gctcgggcgt
     1021 gcccggcgtg ccccggaagt acttctgggt gacgatgttg gtggcgttga gcgaccaggt
     1081 cctggggaac tccacgccga gctgctcgaa cgcgacggat ccgtcctggt agttggtcag
     1141 ccgggcgtcc cggcgctccc actcgagctc gtcgtagggg tgggcacccg ggcgggtgaa
     1201 gaagcggcgg atgccgatgc cggtgcgttc cggagcgatg gccatgtgat tgccctccaa
     1261 ggggtcgagt cgtcttcagc gtgggtcgtg atgccgaggg tcgctacgtc cagctcctcg
     1321 gctgacagct ccccgaccac taggtcttgt ggtcatcggc ccccgaaccc ggtgaccacc
     1381 acaagatgta ggaggcatta catcactgga attacagcga tgtcaatgag caccacagca
     1441 cgaaacagca ggtcaggggg cgttcgcgat cgttagcact cgtgatttcg tgcacgttcg
     1501 cgttcacccc caaggtttcg tccagcccac cacagacacg acgtgagaac caccgtccgc
     1561 tgcccgtgct gtggtgctca gacactcggg tcaacctacg aagaccccgc tgtggaacgg
     1621 aaggggaaca actctgtgaa ttgcgggtgg aattccggtg ctgcgtccac agccggggcc
     1681 tcgagcctgt ggatgtgtgg acaggctgtg gacggcggtg gaaagcctgt gcgctaccgg
     1741 tgggtaacca gcgcggcttc cgccggctcc actgccacga cctcgaccgg gatgccgttc
     1801 agcaccgcgg ttcccgagac cgggtcgaag aggtccgtgt cagcgaggac gttgctgttc
     1861 acgccggcgt gctcggcggc gaccgagagc cgggcgccgt cgaggtcgtg gccccatccg
     1921 tgcgggaggc tgaccacgcc ggccatcacc gcatcggtga cctccaccgg tgccaccacg
     1981 gcaccgaccc gggagcggac ctcggcctgt cccccgtcga ccaggccgcg ctcggccgcg
     2041 tcgtcggggt ggacctggag ggtgcagcgg ggcttgccct tcaccagcac ctccacgttg
     2101 tgcatccagg agttgttcga ccgaaggtgg cggcggccga tgagcagcag gccgtcgtcg
     2161 acctcgtcga gcgcgggcac catgcgggtg gcgacgtcgg cgagcagcac ctcgggggcg
     2221 aggtcgatca tgccgtcggg ctggcgcagc gcctcgggca tccggggctg cagcggaccg
     2281 aggtcgacgc cgtgggggtg gtcgagcagc acgtcgaggc tgagcccgtc gggccgggcc
     2341 ccgaacccgt cgccgtaggg cccggtgcgc agcatcaggt ccagggtccg ctcgggaccc
     2401 acccgggggg ccagcgcctc gagcagctcc tcgacgtcgc gcccgtgcac cagaccgtgc
     2461 tcgtcggcca ccgcggactc gaccatcgag cggatggcgg cgtcgtcgac gagggccggg
     2521 tcggcctcgg cggggtccac cccctgcagg atcagcgaca gggtgcagag gatctcccac
     2581 tcgtcgggcg caccctcgtc gaggggcagc accggtgggc tgtagttggc cacgttgcgc
     2641 agggcgagct ggagcaaggc cacgtcgtag tggctcttct gcagtgccga gggtgccggc
     2701 aggatgacgt cggcgtggcg ggtcgtctcg ttcaggtaga tgtcgatggc cacgacgaag
     2761 tcgagcgagg cgaacgctgc gtcgaggcgg accgagtccg gcgtcgacag gatcgggttg
     2821 ccggccacgg tgatcatcgc cttgatctgg ccctcacccg gggtctcgat ctcctcggcc
     2881 agggcgacga ccgggagctc cccgaaggtc tcgggcaacc cccgcacccg ggaggtgcga
     2941 cgcccgagcc ggatcccacg cccgtagcga cccggtcccc tcgtgttggc ggcgccggcg
     3001 gcggccttgg tgaacatggc cccgcccggg cggtcgaggt tgccggtgca gatgttgagc
     3061 gcgtcgacca gccacgacgt gaccgtcccg tacggtgaca cggtcgtgcc gatgcgggcg
     3121 tagacgacgg ccttcggtgc gtcggccagc tcgtgggcga gccggcggat cgcctcggcg
     3181 tcgatgccgc agcggccggc gacggcctcg ggggtgaaca gggcgaccgc cgcctgcagc
     3241 tcgtcgaccc gggcgacgtg ctcggccacg gtgccgaggt cgacccgatc ggcggcgaag
     3301 agctcctgga gcatggcggc gagcaggaat gggtcggcgc cgggccggat cggcacgtgg
     3361 gcgctggcct cctcggcggt cttggtgcgg cgggggtcga cgaccacgac gcgcccgccc
     3421 cgctcgagga tcgcccggag ccggccgggc cagtcggggg cggtggcgaa gctgccgttg
     3481 gacgcgaacg ggttggcccc gaggatcagc aggtggtcgg tgcggtcgat gtcgggcacc
     3541 gggttggtga gcacgttgcc gaagatcagg cccgaggaga tctccttggg ccgctggtcg
     3601 acggtggagg ccgagaacac gttgggcgag ccgagggcgc gcaggaacgc ccggttgtag
     3661 aggagggctc cgaggttgtg ggcgttgggg ttgccgacgt aggcgccgac cgccgaccgg
     3721 ccgtgctcgt cgatgacccg accgaggccg tcggccaccg cctgccacgc ctcggcccag
     3781 gtggccggct cgagccggtc gccgcgccgc acgagcggct ggcgcaggcg gtcggggtcc
     3841 tcgtgcaggt gcttgagcgt ggagcccttc gggcacaggt agccgtggct gaacacgtcg
     3901 aggcggtcgc cgcggatgcg caccacctgg ccgtctcgca ccgtcacgtc gaggccgcag
     3961 ctggcctcgc acagcgggca ggtgcgctgc acggtgacag cgtcgtccgc atccgccgga
     4021 gcggcggacg ggttcggtgc ggtcgtggtc atgacgtctc ctcgtgtccc ccggtggtgc
     4081 cgccatcgtg gcactcctcg atgggtgggt acggttcgcg gatgcgccag ctgctccccg
     4141 tgcccggcga cgtcgatccg atcgccgcct acctggcggc gccacggccg gcgcccgcag
     4201 ctcggccgtg ggtggcgttg agcatggtca ccagcctcga cggcgccacc gccgtcggcg
     4261 gacgctccgg accgctcggg ggcgagccgg accgggcggt cttccgggcg gtgcgcgcca
     4321 tcgccgacgt ggtgctggtc gcggcaggca cggcgcgcac cgagcgctac ggccccgtgc
     4381 ggttgtcggc gcctaccgcc gcggcccgcg ccgaggccgg gcgctcggcc gacccgcccc
     4441 gcctggtggt cgtctccggg agtctcgacc tcgacctgtc acgagccgcg gaggggccgg
     4501 tgcggccctt ggtgctgacc accgaggatg ccgatccgac ccgccgagcg gcgctcgagg
     4561 agct
//
LOCUS       DATISU010000081         5672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_516186, whole genome shotgun sequence.
ACCESSION   DATISU010000081 DATISU010000000
VERSION     DATISU010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516186"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..982)
                     /locus_tag="VLR27_03545"
     CDS             complement(164..982)
                     /locus_tag="VLR27_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02546.1"
                     /translation="MTRTRASVTLLLAAAMALVLAVPVGAAKGDNNGGGKGGGKPSTS
                     VPDDTTTTEAPTTTEAPTTTAAPTTTVPTSDRDPILFVHGWNSSGSTWDTMVSRFLAD
                     GYTADELMAFSYNTSQSNRTTAAEIADRVATLLEVNRATQVDIIAHSMGSLSSRHYVK
                     FLGGADGPVDTWVSLGGPNHGTDTAYFCWSTACTEMWPGSSFLDDLNADDETPGSIVR
                     YATWWSPCDSVINPDSSVPVSGGAVNTQTACIGHSDLHQDATVYGQVAAFIDAE"
     gene            complement(1040..1483)
                     /locus_tag="VLR27_03550"
     CDS             complement(1040..1483)
                     /locus_tag="VLR27_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HSP02547.1"
                     /translation="MPLIGEYEPSPSDWVREQVETYERTNGKEGNTLLDTGLPVIIVT
                     TRGHKSGKLRKTPLMRVEHEGEYALVASKGGAPEHPEWYHNLVADPTAVMIQDGPEPW
                     DAIVRQVEGEERAEWYERAVAAFPPYAEYQEKTDREIPVFIASRP"
     gene            complement(1518..4424)
                     /locus_tag="VLR27_03555"
     CDS             complement(1518..4424)
                     /locus_tag="VLR27_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSP02548.1"
                     /translation="METREVRGGGATRAPGRASNVFLVAGLAVAALTVVVGDPIRGWL
                     LAAASLAAAVAATVAVRRDPDRSNPAWFFMAGAWLLVPAYVIWYPLTVAWDLSLTSPA
                     ITDWLFLSAYSLFLLGLSKIIGRRSTTDRWLDVLDTLIIGVGFGVLAWIIFIGPYLDA
                     DGMTLPARLVTISYTVVNLLLLGAIVRMLVQGGISSRGDRLLTAWVVAQLGADLLYAM
                     MTLSGTFQVQHQVVSLYPVSFVLFGAALLHPTTMSPPTVEADRSIRGASRFALVVPAA
                     LIAPLALMALGLRDGSGDVVIVSMLAAALFGLVLWRVWLLFVDVEEHRRTQEQLTRSI
                     EQERRRTIENQELLASLSERQLLTDRLSRIQRKISTRAPLQEVLDAITQGAADLVHAD
                     VAALRFVDEDDPDFMVIVSSVGVDEALADEVRRSPVGVGVGGQAIVTNKLCIQDAYTG
                     WDNAIPQFATGGLQTALATPVHLGAAPIGSLVVATHRSDRRYSTAEQDALISFAEHVS
                     IALNDARSVRKMNEALEQAVHQAMHDELTGLPNRACFYDRAELALKAAARTGTATAVL
                     LLDLDRFKEINDTLGHRYGDRVLCAIEPRLAPLLRASDTLARLGGDEFCVLLPAVAGL
                     DEAMEVAHRLTRALEEPLDVDGMTLVVEASCGVTVAPDHGRTADLLLQRADIAMYAAK
                     RSHQSVSPYEDELDRNTPDRLALLGELRHAVAKEELVLHFQPQIDLATGEIEGAEALV
                     RWQHPRRGLLAPDVFIPLAEDTGFIHPLTTWVLDATLAQVRRWIDDPDEHGIGPDFTV
                     AVNLSTHSLLDDCFATEVSVALDRAGVPPSRLALEITETTLMADPERATRVLTTLAER
                     GVRFAIDDFGTGYSSLASLKDLPVHDLKIDKSFVAGMDEGSDDAVIVRSVIDLGHTLG
                     LRTIAEGAETQEVLSRLADLGCDSAQGYAVARPMTACELVTWLRARAAAGVAVA"
     gene            complement(4505..5269)
                     /locus_tag="VLR27_03560"
     CDS             complement(4505..5269)
                     /locus_tag="VLR27_03560"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSP02549.1"
                     /translation="MAALTSTRDGDVVTITLTAPERRNPLSTSTLRELGSALDEAAGS
                     DALAVVLASTGSVWSTGHDLKEMSTLDEAALRELFTTCTKVMRTIESMPQPVVARVHA
                     LATAAGCQLVASCDLAVAGASARFATPGGKGGLFCHTPMVAVARNVGRKRAMELALSG
                     DEIDAETADDWGLVNQVVPDDELDGAVADLVRRVTRGSRASKGQGKQTLYAQMDLTID
                     QAYEVAIDVMARNAASGDGAEGIAAFAEKRPPRFAP"
     gene            complement(5290..>5672)
                     /locus_tag="VLR27_03565"
     CDS             complement(5290..>5672)
                     /locus_tag="VLR27_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSP02550.1"
                     /translation="FSIPEIDLGIPLAWGGIPRLVREIGPARTKELVMTGREFSPEEA
                     LTAGFLNRIVDDDDLDSAVTTLVEQLVTKPKLGLLATKAHTNAVTESMVGTGRSWADA
                     DGLEAGLRDPEGRAVRQAYLDRKG"
BASE COUNT          806 a         1896 c         2141 g          829 t
ORIGIN      
        1 ccagttcaag cgctcggcca tgccgaacgg cccgaaggtc tccggcggcg gcgccctgtc
       61 accccgtggc gactggcgcg caccgtcgga cgggaccgcc acgcccttcc tcgacgagct
      121 cgaacgcaac ctgccgccgg cgtagccggc ggcaggtcca ccgctactcg gcgtcgatga
      181 aggcggcgac ctggccgtag acggtggcgt cctggtgcag gtcgctgtgc ccgatgcagg
      241 cggtctgggt gttcaccgca cctccgctga ccggcacgct gctgtcgggg ttgatcaccg
      301 agtcgcaggg cgaccaccac gtggcgtaac gcacgatcga gccgggcgtc tcgtcgtccg
      361 cgttcaggtc gtcgaggaac gacgagccgg gccacatctc cgtgcacgcc gtcgaccagc
      421 agaagtaggc ggtgtcggtt ccgtggttgg gtccgccgag ggacacccag gtgtcgaccg
      481 gaccgtccgc gccgccgagg aacttcacgt agtggcggga cgagaggctg cccatggagt
      541 gggcgatgat gtcgacctgg gtggcgcggt tgacctcgag cagggtggcg acccggtcgg
      601 cgatctctgc cgccgtggtg cggttggact gcgaggtgtt gtacgagaac gccatcagct
      661 cgtcggcggt gtagccgtcg gccaggaagc ggctgaccat cgtgtcccac gtgctgccgc
      721 tcgagttcca gccgtgcacg aacaggatcg gatcccgatc cgaggtcggc accgtcgtgg
      781 tcggcgctgc cgtggtggtc ggcgcctcgg tcgtcgtggg ggcctcggtc gtggtggtgt
      841 cgtccgggac gctggtggac ggcttcccgc ctcccttgcc cccgccgttg ttgtcgccct
      901 tcgccgcgcc gacgggcacg gccaggacca aggccatcgc tgccgccagc agcagcgtca
      961 cgcttgctcg agtgcgggtc atgggagaca ccccttcagg tcgtacctgt gtcggacgtc
     1021 acactaccca cgcgtcgggt caggggcggc tggcgatgaa gaccgggatc tcccggtcgg
     1081 tcttctcctg gtactcggcg tacggcggga aggcggcgac ggcccgctcg taccactcgg
     1141 cccgctcctc gccctccacc tggcggacga tggcgtccca cggctcggga ccgtcctgga
     1201 tcatgacggc ggtcgggtcg gcgaccaggt tgtggtacca ctcggggtgc tcgggcgcgc
     1261 cgcccttgga ggcgaccagg gcgtactcgc cctcgtgctc gacccgcatc agcggcgtct
     1321 tgcggagctt gccggacttg tggccccggg tggtcacgat gatcaccggc aacccggtgt
     1381 cgaggagggt gttgccctcc ttgccgttcg tgcgctcgta ggtctcgacc tgctcgcgca
     1441 cccagtccga cgggctcggt tcgtactcac cgatcagagg catgtcccga acataggccc
     1501 gatcggggtg ggcgtgctca ggcgaccgcg acgccggcag cggcgcgagc ccgcaaccag
     1561 gtgaccagct cgcaggccgt catcggccgg gccacggcat agccctgggc gctgtcacag
     1621 cccaggtcgg ccaggcggga cagcacctcc tgggtctcgg cgccctcggc gatggtgcgc
     1681 aggccgaggg tgtggccgag gtcgatcacg gagcgcacga tcacggcgtc gtcggagccc
     1741 tcgtccatcc cggccacgaa ggacttgtcg atcttcaggt cgtggacggg caggtccttc
     1801 agcgacgcga gcgaggagta gccggtcccg aagtcgtcga tggcgaagcg caccccccgc
     1861 tcggcgagcg tcgtgagcac ccgggtggcg cgctcggggt cggccatgag ggtcgtctcg
     1921 gtgatctcca gcgcgaggcg cgagggcggc accccggcgc ggtcgagggc cacgctcacc
     1981 tcggtggcga agcagtcgtc gaggaggctg tgggtggaca ggttcacggc cacggtgaag
     2041 tccggtccga tgccgtgctc gtcgggatcg tcgatccagc gccggacctg ggcgagggtg
     2101 gcgtcgagca cccaggtcgt cagcgggtgg atgaagccgg tgtcctcggc gagggggatg
     2161 aacacgtcgg gggcgagcag gccccgtctg gggtgctgcc agcgcaccag cgcctcggct
     2221 ccctcgatct ctccggtggc caggtcgatc tgaggctgga agtgcaggac cagctcctct
     2281 ttggccacgg cgtggcgcag ctcgccgagg agggcgaggc ggtccggcgt gttgcggtcc
     2341 agctcgtcct cgtaggggga cacgctctga tgcgagcgct tggcggcgta catggcgatg
     2401 tcggcgcgct gcaggagcag gtcggcggtg cggccgtgat cgggagccac cgtgacgccg
     2461 cagctggcct ccaccacgag ggtcatgccg tcgacgtcga ggggctcctc gagcgctcgg
     2521 gtcaggcggt gggcgacctc catggcctcg tcgaggccgg ccaccgcggg gagcagcacg
     2581 cagaactcgt cgccgccgag ccgggccaac gtgtccgatg cccgcagcag cggcgcgagc
     2641 cggggctcga tggcgcacag cacccggtcg ccgtagcggt ggccgagcgt gtcgttgatc
     2701 tccttgaacc ggtcgaggtc gaggagcagc accgccgtgg ccgtgccggt ccgtgccgcc
     2761 gccttcaggg cgagctcggc ccggtcgtag aagcacgccc ggttgggcag cccggtgagc
     2821 tcgtcgtgca tggcctggtg cacggcctgc tcgagcgcct cgttcatctt gcgcacggac
     2881 cgggcgtcgt tcagggcgat gctgacgtgc tcggcgaagc tgatgagggc gtcctgctcg
     2941 gccgtggagt agcgccggtc cgatcggtgg gtggccacga ccaggctgcc gatgggtgcg
     3001 gccccgaggt ggaccggtgt ggcgagggcg gtctgcaggc cgccggtcgc gaactggggg
     3061 atggcgttgt cccaccccgt gtacgcgtcc tggatgcaga gcttgttggt gacgatggcc
     3121 tggccgccga caccgacccc caccggggat cgccggacct cgtcggcgag ggcttcgtcg
     3181 acgccgacgg aggagacgat gaccatgaag tccgggtcgt cctcgtcgac gaagcgcaag
     3241 gcggcgacgt cggcgtggac gagatcggcc gcaccctggg tgatggcgtc cagcacctcc
     3301 tggagcgggg cacgggtgga gatcttgcgc tggatgcgcg acaggcgatc ggtcagcagc
     3361 tggcgctcgc tcagggaggc gagcagctcc tggttctcga tcgtgcgccg acgctcctgc
     3421 tcgatcgacc gggtcagctg ctcctgggtg cggcggtgct cctcgacgtc gacgaacagc
     3481 agccacacgc gccagagcac gaggccgaag agggcggccg ccagcatgga gacgatgacg
     3541 acgtcgccgc tgccgtcgcg cagcccgagg gccatgagcg caaggggggc gatgagcgcg
     3601 gccggcacca cgagggcgaa gcggctcgcg ccccggatgg accggtcggc ctcgacggtc
     3661 gggggcgaca tcgtcgtggg gtggagcagg gcggcgccga acagcacgaa ggagaccggg
     3721 tagaggctga cgacctggtg ctggacctgg aacgtgccgc tcaaggtcat catggcgtag
     3781 agcaggtcgg cgccgagctg ggccacgacc caggcggtga gcagccggtc gccgcgggag
     3841 gagatgccgc cctgcaccag catccgcacg atggcgccga gcaggagcag gttcaccacc
     3901 gtgtaggaga tcgtcaccaa ccgggcgggg agcgtcatgc cgtcggcatc gaggtacggc
     3961 ccgatgaaga tgatccaggc cagcacgccg aagcccacac cgatgatgag cgtgtcgagc
     4021 acgtcgagcc agcggtcggt cgtgctgcgc cggccgatga tcttgctgag cccgaggagg
     4081 aacagcgagt acgcgctgag gaagagccag tcggtgatcg ccggcgacgt gagggacaga
     4141 tcccaggcga ccgtcagcgg gtaccagatc acgtaggcgg gcacgaggag ccatgcgccg
     4201 gccatgaaga accaggccgg gttcgagcgg tccgggtcgc gccggacggc gacggtcgcg
     4261 gcgaccgcag cggccaggct cgcggcggcc agcagccagc cgcggatcgg gtcgccgacc
     4321 acgacggtca gcgcagcgac cgcgaggccg gcgaccagga agacgttgga ggcccgaccg
     4381 ggcgcacgcg tcgcaccgcc accgcgcacc tcccgggtct ccatgtccca atcatcggcc
     4441 gcgtccgggg ccgacatgag ggtcggaccg aggaaaaccg gcttcggacg cccgattcgc
     4501 ccgatcaggg ggcgaagcga ggtgggcgct tctccgcgaa tgccgcgatc ccctcggccc
     4561 cgtcacccga tgcggcgttg cgggccatga catcgatggc cacctcgtag gcctggtcga
     4621 tggtcaggtc catctgggcg tagagggtct gcttgccctg ccccttcgac gcccgggagc
     4681 cccgggtcac ccgacggacg agatcggcca cggccccgtc gagctcgtcg tcgggcacca
     4741 cctggttcac cagcccccag tcgtcggcgg tctcggcatc gatctcgtcg cccgacaggg
     4801 cgagctccat ggcgcgcttg cgtccgacgt tgcgggcgac ggccaccatc ggcgtgtggc
     4861 agaacagccc gcccttgccg cccggggtgg cgaagcgggc ggacgcgccg gccaccgcca
     4921 ggtcgcagct ggcgacgagc tggcagccgg cggcggtggc gagggcgtgg acccgggcca
     4981 ccaccggttg gggcatcgac tcgatggtgc gcatcacctt cgtgcacgtg gtgaacagct
     5041 cgcggagcgc ggcctcgtcc agcgtcgaca tctccttcag gtcgtgcccg gtgctccaca
     5101 ccgacccggt ggaggcgagc accacggcca gggcatcgga gccggcggcc tcgtcgagcg
     5161 cggatccgag ctcgcgcagg gtcgacgtcg agagcgggtt gcgacgctcc ggcgcggtga
     5221 gggtgatggt gacgacgtcg ccgtcgcggg tcgaggtcag ggcggccatg ccccgatgct
     5281 cgcgcgccgt cagcccttgc ggtcgaggta cgcctggcgc acggcccgac cttccgggtc
     5341 gcgcagcccc gcctcgaggc cgtcggcgtc tgcccacgag cgaccggtgc ccaccatgga
     5401 ctcggtcacc gcgttggtgt gggccttggt ggcgagcagg ccgagcttgg gcttggtcac
     5461 cagctgctcg acgagggtgg tcacggccga gtcgaggtcg tcgtcgtcca cgatgcggtt
     5521 caggaacccg gcggtcaggg cctcctccgg cgagaactcc cggccggtca tcaccagctc
     5581 cttggtgcgg gccgggccga tctcgcggac caggcggggg atgccgcccc aggccagcgg
     5641 gatgccgagg tcgatctccg ggatcgagaa gc
//
LOCUS       DATISU010000082         3766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_190093, whole genome shotgun sequence.
ACCESSION   DATISU010000082 DATISU010000000
VERSION     DATISU010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190093"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..355
                     /locus_tag="VLR27_03570"
     CDS             104..355
                     /locus_tag="VLR27_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02551.1"
                     /translation="MARRRREEMSTVQLVVGFAVLLAVVVGGRLLVVWLADGSSPTRL
                     VVSLTIVAVFIGIPAAYGLRGLLRDDPDGGDEVDRNPDR"
     gene            382..1275
                     /locus_tag="VLR27_03575"
     CDS             382..1275
                     /locus_tag="VLR27_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSP02552.1"
                     /translation="MPGKTELVLLSDEVWERTRERLDGLTDEEYFWEPAPGCWSVRQR
                     RDGRWAIDQALPAPDPAPLTTIAWRLWHLIDMYGENRAPEWLDVPAQGAPVGLDAPDA
                     APPATAAGALAMLERAHARWDAHLHLVAEEQLAMPIGPVGQDYAERSRTSYVLHMLDE
                     FIHHGAEISLLRDLWRWQPGRLDDGSARERAMRGDKDLLDEVDAEQATELLDTAADNG
                     RWELVLGLVERGARVRTSGRTPLHLAAGAGEVEVVRALLDHGGDPTATDPVFEATPVQ
                     WARYLGQPATAEVLEGRAPEG"
     gene            1409..2620
                     /locus_tag="VLR27_03580"
     CDS             1409..2620
                     /locus_tag="VLR27_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HSP02553.1"
                     /translation="MPDLAEAPFLDIFDPDLDADPEPVYASLREETAVARTPLGAVVL
                     RRDEIHRLLGDRRLISSIPHLVRLQGAGDGPVGALLESSVIAVDGDDHTRLRRLVSRS
                     FTPSAANRHRPLMRSLVQELVDGFTVDADGRGRCEFVSELADHYPVRVICSVLGVPDE
                     DHEQFGTWAESITYMLSLELSAHLDEVVHAVQALGDYVVDLVEERRRHPQDDLLTSLV
                     QASEDGDRLSNDELLSMVGGLLFAGVDTTRNQLGLAMHTFCERPDQWELLRSRPELTP
                     AAVNETMRLFGAVPGIPRVTLEDVEVDGWCIPAGTIVFLSLASANRDPRHFDDPMRFD
                     ITAEREPHLTFGGGPHYCLGANLAKAEMEEALLVLAASMPDLRLDGEPVWRTSTGIGG
                     PTHLPLSFAAG"
     gene            complement(2622..2915)
                     /locus_tag="VLR27_03585"
     CDS             complement(2622..2915)
                     /locus_tag="VLR27_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HSP02554.1"
                     /translation="MLDITADGGVVIDVHVQPGGSRDEVVGRHGDALKVKVSAPPEGG
                     KANAAVCRLLADAFDVPASSVSVTAGAKSRRKRVRIDDLDPTFASGRLDQLLD"
     gene            3130..3426
                     /locus_tag="VLR27_03590"
     CDS             3130..3426
                     /locus_tag="VLR27_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02555.1"
                     /translation="MSDADRPGDGDGLSGPERDAMVASLLGITPERLRTIRRVHRKRA
                     DGATPFGGGGRRAEWWQCVDQLDDDELFAAFRRREVRARRLSRPPARRPRSAIL"
     gene            complement(3383..>3766)
                     /locus_tag="VLR27_03595"
     CDS             complement(3383..>3766)
                     /locus_tag="VLR27_03595"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HSP02556.1"
                     /translation="EFGRTRQGHECAPALSLEEYFSTGPERERPIFEAVHEHLSSLGD
                     VYVEPVSVGIFFKIHTTFAQLRPMTKWVALSFFSPQRIDHPRIARKPAEYSGRWHHVV
                     NVREADEIDDLVRGWLTEAYVLEVG"
BASE COUNT          533 a         1345 c         1352 g          536 t
ORIGIN      
        1 tccactggtg caaccagggc tcggccgtct ggaacaacag cggtgacacc gcgttcctgc
       61 tcgacccggc cgggaacatc gtcacctccc aggacggctg atcgtggccc ggcgccgacg
      121 cgaggagatg agcaccgtcc agctcgtcgt cggcttcgcc gtgcttctcg ccgtcgtcgt
      181 cggagggcgc ctgctggtgg tgtggctggc tgacggttcg agcccgacgc ggctcgtcgt
      241 gtcgctcacg atcgtggccg tcttcatcgg gatccctgcg gcctacgggt tgcgtgggct
      301 gctgcgggac gatccggacg gtggcgacga ggtcgaccga aaccccgacc ggtgaccacg
      361 tggcgggcgt accgttcggg catgcccggg aagacggagc tggtgttgct gtcggacgag
      421 gtgtgggagc gcacgcggga gcgcctcgac ggcctcaccg acgaggagta cttctgggag
      481 ccggcgcctg gttgctggtc ggtgcgccag cgcagggacg gccggtgggc gatcgaccag
      541 gccctgcccg cgcctgatcc ggcaccgctc acgaccatcg cctggcggct gtggcacctc
      601 atcgacatgt acggcgagaa ccgggcgccc gagtggctgg acgtgccggc ccagggggcg
      661 ccggtcggcc tcgacgcgcc cgatgccgcg ccgccggcca cggccgccgg cgccctcgcc
      721 atgctcgagc gggcgcatgc ccgctgggac gcgcacctcc acctggtggc cgaggagcag
      781 ctcgccatgc ccatcggccc ggtcggccag gactacgccg agcggagcag gacgtcctac
      841 gtgctgcaca tgctcgatga gttcatccac cacggtgccg agatctcgct gctgcgcgac
      901 ctgtggcgct ggcagcccgg ccggctcgac gacggctccg ctcgcgagcg ggcgatgcgc
      961 ggcgacaagg acctgctcga cgaggtggac gcggagcagg cgaccgagct gctcgacacc
     1021 gcagcggaca acggtcggtg ggagctcgtc ctcggtctgg tcgagcgcgg cgcgcgcgtc
     1081 cgcacctcgg gtcgtacgcc gctgcacctg gctgccggcg ccggcgaggt ggaggtcgtg
     1141 cgggcgctgc tcgaccacgg cggcgacccg accgccaccg accccgtgtt cgaggccacg
     1201 ccggtgcagt gggcccggta cctcggccag ccggcgacgg ccgaggtgct ggagggtcgc
     1261 gcgccggagg gctgagagcc tgctcggagc caggtgtgac acatgtgcgg tcgccacgct
     1321 ggcagcaccg tcgtaaagtg cgcgaaacga ccggcgatgt ccgggccggc actggggcgg
     1381 tcgcggaggt cggtcaggag gtggtcctgt gcccgacctc gccgaggcgc cgttcctcga
     1441 catcttcgac cccgatctcg acgccgaccc agagccggtg tacgcctcgc tccgggagga
     1501 gaccgccgtc gcccggacgc cactcggtgc ggtggtcctc cgacgggacg agatccaccg
     1561 gctgctcggc gatcggcggc tcatcagctc gatcccccac ctcgtccggc tccagggcgc
     1621 cggcgacgga cccgtcggcg cgctgctgga gtcgtccgtc atcgcggtcg acggtgacga
     1681 ccacacgcgc ctgcgccgac tggtgagccg ttcgttcacg ccgagcgccg ccaaccggca
     1741 ccgcccgctc atgcgctcgc tcgtgcagga gctggtcgac ggcttcaccg tggatgccga
     1801 tggcagaggt cggtgcgagt tcgtctcgga gctcgccgac cactacccgg tgcgggtcat
     1861 ctgcagcgtg ctcggcgtcc ccgacgagga ccacgagcag ttcgggacct gggccgagtc
     1921 gatcacctac atgctctccc tcgagctgtc cgcccacctg gacgaggtcg tccacgccgt
     1981 ccaggcgctc ggggactacg tcgtcgacct ggtcgaggag cgccggcggc acccgcagga
     2041 cgacctgctc accagcctcg tgcaggcctc cgaggacggc gaccggctga gcaacgacga
     2101 gctgctgtcg atggtcggcg gcctgctgtt cgccggcgtc gacaccaccc gcaaccagct
     2161 cggcctggcc atgcacacgt tctgcgagcg ccccgaccag tgggagctgc tccgcagccg
     2221 gcccgagctg accccagcag cggtcaacga gaccatgcgg ctgttcggcg cggtgccggg
     2281 catcccccgg gtgacgctgg aggacgtcga ggtggacggg tggtgcatcc cggccggcac
     2341 gatcgtgttc ctgtccctcg cctcggccaa ccgcgatccc cggcacttcg acgacccgat
     2401 gcggttcgac atcacggccg agcgggagcc gcacctcacc ttcggcggcg ggccgcacta
     2461 ctgcctgggc gccaacctgg cgaaggccga gatggaggag gcgctgctcg tgctcgccgc
     2521 ctcgatgccg gacctgcgcc tcgacggcga gccggtgtgg cggaccagca ccggcatcgg
     2581 cggaccgacc catctgccgc tgtcgttcgc agccggctga ctcaatcgag cagctggtcc
     2641 aagcggcccg atgcgaacgt cgggtcgagg tcgtcgatcc gcacccgctt tcgccggctc
     2701 ttcgcaccgg ccgtcaccga caccgacgac gccggcacgt cgaacgcatc ggccagcagg
     2761 cggcacaccg cggcgttcgc cttgccgccc tccggcggtg cggacacctt caccttcagg
     2821 gcatcgccgt ggcggccgac gacctcgtcg cgcgagccac cgggctgcac gtggacgtcg
     2881 atgaccacgc cgccgtccgc cgtgatgtcg agcacgcccc catcgtgccc gtcggagccg
     2941 gcacgggggt ccacccgctc ctcgacggat ggtggcaccg tcttggtgtg acggagaacg
     3001 tcacgagatc gatgcacgca atggtgcaga cggcccatgg caggagcatg cggagggcgt
     3061 agaacgggtg tgacggaagc gccacccaac gtggtggctc accggacgac gagatcgtcg
     3121 aggcacgaca tgagcgatgc agaccgacca ggggacgggg acgggctgag cggccccgag
     3181 cgagacgcca tggtggcgtc gctgctcggc atcaccccgg agcgactccg caccatccgg
     3241 cgcgtccacc gcaagcgggc cgatggggcg acgcccttcg gtggcggagg tcgccgggcc
     3301 gagtggtggc agtgcgtcga tcagctggac gacgacgagc tcttcgccgc cttccgccgg
     3361 cgcgaggtgc gggcccgccg gctcagccga cctccagcac gtaggcctcg gtcagccatc
     3421 ctctgaccag gtcgtcgatc tcgtcggcct cccgcacgtt gacgacgtgg tgccagcggc
     3481 cgctgtactc ggccggcttg cgagcgatgc gcgggtggtc gatgcgctgc ggggagaaga
     3541 acgacagcgc cacccacttc gtcatgggtc ggagctgggc gaacgtggtg tggatcttga
     3601 agaagatgcc caccgagacg ggttccacgt agacgtcgcc gaggctcgac aggtgctcgt
     3661 gcaccgcctc gaagatcggc cgctcccgct cgggcccggt cgagaagtac tcctcgagcg
     3721 acagcgccgg cgcgcactcg tgcccctgac gcgtgcggcc gaactc
//
LOCUS       DATISU010000083         1883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_748974, whole genome shotgun sequence.
ACCESSION   DATISU010000083 DATISU010000000
VERSION     DATISU010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748974"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VLR27_03600"
     CDS             <1..823
                     /locus_tag="VLR27_03600"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HSP02557.1"
                     /translation="VEEDEGTTTVLVRPDDLAPDPVLAGLLALLDDPAAPVRAGGLDL
                     VDPVVDDDADAVEPVFGSGADLSDIAPRVLATAGQLDSYQGLIGPESSRADDLRLQVA
                     TAVATTTPSARRDAAMDTVEEVLGTAFGSVRLSGERNLNLTSRRGTLPVTVENANPFP
                     VDVVIRTTSDRLRFPDGRDLPITVEGGDATRVDVPVEALATGSVPVSVELWTPDDRIR
                     LDGRQLNVRSTAVSGVGLLLSLGALVVLVVWWARSWRRNRRAPATTSGPAAEPMG"
     gene            892..1728
                     /locus_tag="VLR27_03605"
     CDS             892..1728
                     /locus_tag="VLR27_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HSP02558.1"
                     /translation="MAGVRIVTDSACDLTDDEATSLGIEVVPLSIRFGSDEFEDRSEL
                     SAEAFYEKMDQRSELPETAAPSPGKFEAAFRRQAEAGADAVVCINLSSDLSATMAAAE
                     NAARAIGDDLDVRVVDSKSITAGLGTQVLLAAQRAADGASADEVVALVEDLRARTHVI
                     GCLDTLENLKKGGRIGGAQAMLGSLLSIKPMIDISTGVVEEAGKHRTRKKSLEALREK
                     VRSSGKVEHLAIMHGMAPDVDVMTKLLDEDGYDYRLGHIGAVIGSHGGPRVVGACWVD
                     PA"
BASE COUNT          262 a          745 c          643 g          233 t
ORIGIN      
        1 cgtcgaggag gacgaaggca cgaccacggt gctcgtccgg cccgacgacc tcgcgccgga
       61 cccggtcctg gccggtctgc tcgccctgct cgacgacccg gccgcgccgg tccgcgccgg
      121 tggcctcgac ctcgtggacc cggtcgtcga cgacgacgcc gacgcggtcg agcccgtctt
      181 cggctccggg gcggacctgt ccgacatcgc cccacgggtc ctcgccaccg ccggccagct
      241 cgactcctac cagggcctca tcggcccgga gtcgtcgcga gccgacgacc tccggctgca
      301 ggtcgccacg gccgtcgcca ccaccacgcc gagcgcgcgg cgcgatgccg ccatggacac
      361 cgtcgaggag gtgctcggca ccgccttcgg cagcgtccgc ctgagcggcg agcggaacct
      421 gaacctgacg tcgaggcgcg ggaccctgcc ggtcaccgtc gagaacgcca acccgttccc
      481 ggtcgacgtc gtcatccgca ccaccagcga ccgcctgcgc ttccccgacg ggcgggacct
      541 cccgatcacc gtcgagggcg gcgacgccac gcgcgtcgac gtacccgtgg aggccctggc
      601 caccgggtcg gtgcccgtct cggtcgagct gtggaccccc gacgaccgca tccgcctcga
      661 cggccggcag ctgaacgtgc gctccacggc cgtcagcggc gtgggtctgc tcctgtcgct
      721 cggcgccctg gtcgtgctgg tcgtgtggtg ggcccgcagc tggcgacgga accgccgagc
      781 gccggcgacg acgtcgggcc cggccgccga gccgatgggc tagacactcc ggcaccgggg
      841 ggtctggcgc cccgcggcga cccgaccgac cgagcaccac cggagagcac catggctggc
      901 gtccgcatcg tcaccgacag cgcctgcgac ctgaccgacg acgaggccac ctccctcggc
      961 atcgaggtcg tgcccctgtc gatccgcttc ggcagcgacg agttcgagga ccgctccgag
     1021 ctctcggccg aggccttcta cgagaagatg gaccagcgca gcgagctgcc cgagaccgcc
     1081 gcaccgtcgc ccggcaagtt cgaggccgcg ttccgccgcc aggccgaggc cggggccgac
     1141 gccgtcgtct gcatcaacct gtcgtcggac ctctccgcca ccatggccgc cgccgagaac
     1201 gccgcccgcg ccatcggcga cgacctcgac gtgcgggtcg tcgactccaa gtcgatcacc
     1261 gccggcctcg gcacccaggt gctgctcgcc gcgcagcgcg ccgccgacgg cgcgtcggcc
     1321 gacgaggtcg tggcgctggt cgaggacctc cgggcccgca cccacgtcat cggctgcctc
     1381 gacaccctcg agaacctcaa gaagggcggc cgcatcggtg gcgcccaggc gatgctcggc
     1441 tcgctcctgt cgatcaagcc gatgatcgac atctccaccg gcgtcgtcga ggaggccggc
     1501 aagcaccgga cccgcaagaa gtcgctcgag gcgctgcgcg agaaggtgcg ctcgtccggg
     1561 aaggtcgagc acctggcgat catgcacggc atggcccccg acgtcgacgt gatgacgaag
     1621 ctgctcgacg aggacggcta cgactaccgc ctcggtcaca tcggtgcggt catcggctcg
     1681 cacggcggcc cccgcgtcgt gggcgcctgc tgggtcgacc cggcctgagc cgacgcccct
     1741 gtccgcgccg gcgactcgcg gcccggtgag ccccgacggg tagcgtcggt ggggtgtcgg
     1801 agcaccccac gcggccgacg accgtgaccc cgaacggcag tcccctcgag cagggcacca
     1861 ccatcggcgg ccgctaccag ctc
//
LOCUS       DATISU010000084         8348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_516406, whole genome shotgun sequence.
ACCESSION   DATISU010000084 DATISU010000000
VERSION     DATISU010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8348
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516406"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VLR27_03610"
     CDS             complement(<1..203)
                     /locus_tag="VLR27_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02559.1"
                     /translation="MAEVLERCETAELHAYWAVAREVHGDDARVEHLARTPSQRRADA
                     LVAIFTRAASAQGCSPEPLVNIVV"
     gene            complement(222..833)
                     /locus_tag="VLR27_03615"
     CDS             complement(222..833)
                     /locus_tag="VLR27_03615"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSP02560.1"
                     /translation="MTDQMEPKVGLACALAVLEDAVGLVQAADLGGLSPREELALVRR
                     LEVLRRRLDHGTDVAAGHLDASAAFSLDGHKTAKGALKAIGRLSGSEAHGRVQTSRAL
                     RRLPLVEAAYAEGRIPTEHVRAIARTISNPRGAEHVADADPIFARHATTLGYDDFVAA
                     LKVWEAFADADGADQGSELSHERRNASLLESGIDGSFRLEAIH"
     gene            1066..1599
                     /locus_tag="VLR27_03620"
     CDS             1066..1599
                     /locus_tag="VLR27_03620"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HSP02561.1"
                     /translation="MTSVEITSSWSQIGFVLLSAGAMLTGIIIYVRIIGLRSFSKMSS
                     FDFAVTVAMGSLLAAVTLSGSSLVDGLVGAGALLAVQAAIAYGRIRFGLSAVVDNQAL
                     LLMAGPTMLEENMRRARVTPDDLRAKLRQANIHRYDQVRYVVLETTGDVSVVQGDGEL
                     DPDVLADVVDAETLFTR"
     gene            complement(1606..2628)
                     /locus_tag="VLR27_03625"
     CDS             complement(1606..2628)
                     /locus_tag="VLR27_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSP02562.1"
                     /translation="MVAGSLVRVGFLGAGFIATFHSKLLRASGVAHERAGVHDLDRDR
                     AEAFVAAAGGFVADSEDEVLGSCDAVFICTWTSDHEERVRRAVDAGLAVFVEKPLSTD
                     LDGARRVTDLLAGTTHQVGLVLRRSPAYGLVQSILEDTERNGRIMSAMFRDDQVLPTG
                     GRYASAWRADASKAGSGVLLEHSIHDVDLIDRLLGPVTSVSGHQRSFHEIDGIEDLVV
                     ATFELEGGGVATLTTVWHDLPDRNSLRYLEIHCERAFISVEEDWFGPVRYTVDGATQE
                     WSGDGLMAECQRRGIPTDLPEGEFIRAVAEGGPATPDAAVALRAHELVDAVYRSAAAG
                     GAPTEI"
     gene            complement(2628..3407)
                     /locus_tag="VLR27_03630"
     CDS             complement(2628..3407)
                     /locus_tag="VLR27_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSP02563.1"
                     /translation="MTRLTKGDRSVAGRVAVITGAASGMGRATAHLFADEGAKVAVLD
                     RTEEGVAAVVDEITGAGGTAAGWAVDVTDGDRVRAVVDEIVDRLGPPDALVNNAGVSL
                     PAPIDGPDFDAQWATTLAVNLTAHTTTIRACLPHLTRNGDGRIVNIASTEGVGGSAYI
                     SPYTASKHGVIGLSRSLAVELGPTGVTVNVVCPGPIHTGMTSEIPDEAKQKFARRNVP
                     MKRYGDPEEVAHATLSLCLPAMSYCTGAVVVVDGGFTTKNN"
     gene            3587..5716
                     /locus_tag="VLR27_03635"
     CDS             3587..5716
                     /locus_tag="VLR27_03635"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02564.1"
                     /translation="MPPVAILAVQQVLYPLPLGVWVRGVLVGGVTALIALGMALIYRS
                     NRIVNFAQGDLGAAPAVLVFLLLTEWGWPYTLAVAAGALAAFALGGVVELAVVRRFFR
                     APRLVLTVATIGLAQLLTGIAVFLPRWFGIEGSFLAPRVEDPFDVRFELAGTIFRGNS
                     VIAFVVTPLLVLGLVVFLRGTKVGIAIRASADRADRAALLGVPVKRLQTLVWAVAALL
                     AFAATFMRAGILGLPVGSVLSLGILLRSLTALLLGGLVHLPVIGLSAVALGVLELGID
                     WHQGGTVPLLGIDLPPLDAVLAVVVLIALVLRRRGDGRSDIDDQSSWQAADEVRPIPT
                     ELARLPEVRWSRVGIAAVLAAVALALPHVLAVDQSLKASAVMVYAILAMSIVVLTGWA
                     GQVSLGQVAFFAIGAATGAKLTHTWDVDLLLAVVGAGIAGAAAAVLVGLPALRYRGLY
                     LAVTTFAFALATTAYFLDRGYFDWVPNQRIERQPLLGRIDISSPTRIYYVVLAGLVLV
                     LVGLRGVRHSRTGRVLIALRDNERGAQAYSVDVVRAKLTAFAISGFVAAFAGALFVHH
                     QQAFGTGPYQPAENLAVFAMAVVGGITSLPGALLGALYLLGTRWFLPLEWQFLASGFG
                     VLLVLMLLPSGLGGLLFDLRDHALRWIAGRRGILVPSLVADRAEPRTDAGDDPTPLPS
                     EAPTPDVAAPAEEPERPVTATTGRTQP"
     gene            5713..>8348
                     /locus_tag="VLR27_03640"
     CDS             5713..>8348
                     /locus_tag="VLR27_03640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP02565.1"
                     /translation="MRRFWQVLAHPKQSLLSITDGAPIYPLIVLFGLNAVDELDRTAF
                     GILVPEIRDEFGLDLQGVLTLIAFVSLCALTLQVPIAMLADKYNRVRIAWIGAAAWAV
                     FSLGTGFATGIVFLALMRAGSGVGRAVVDPTHNSLIADYYEPGIRPRVFSAHRAANAV
                     GAFIGPLSAGLLAAAYGWRTPFFVFVVPTLVFVVLAWRLQPPVRGGHERRAMGASEEA
                     IATEEDAPSFAEGWRLVWKIETLRRIWYSLPFLAASLIGFVSLASLLYDEVFDLDERA
                     RGFVAASTEPIQLVGLVIGARVATRLLAEGPEKVLGFLARTSVLVSGCLVVFALAPNI
                     WVAIAANMVITGALAVIGPGILACLSLAIPPRARSIGFSVASLWVIPGVLLLPIIGAV
                     GDRWGIRTGMLLMVPMFLIGGMVISTTKRTITGDILQVWKAAAARSEALLERRAGRSK
                     LLLIRDVEVSYGNVQVLFGVDMEVGEGEIVALLGTNGAGKSTLLNAISGVTEAHRGAI
                     IFDGRDITHAPPNEIAAMGIAQVPGGKGVFPSLTVGENLELAAWLHRKDGDHGGLARV
                     HELFPILEARRDAPAGDLSGGQQQMLALAMAFLSRPRLLMIDELSLGLAPVIVSQLLP
                     VVEELRSQGTTVILVEQSVNVALDVAEKAFFLEKGEVRFSGPTADLLSRPDVLRSVFL
                     AGQLGAATGDGDGRSPARTQERVPIPDDGTPPALQTLDVTRSFGGIMAVDHVSIEVAP
                     NEILGIIGPNGAGKTTLFDLLSGFVPVDSGRVLLGGADVTEQGAAGRARRGLGRSFQD
                     ARLFPSLTVQEALAVSMERWVTSRDPVSAALHLPNAYDAEQKVARRVEELVELLGLGA
                     YRSKFIRELSTGTRRVVDLAGLL"
BASE COUNT         1065 a         3141 c         2828 g         1314 t
ORIGIN      
        1 accacgatgt tgaccagcgg ctccggtgag cacccctggg cgctggcagc acgggtgaag
       61 atcgccacca gggcatcagc ccgacgctgg gacggggtgc gggccaggtg ctcgacccga
      121 gcatcatcac catgcacctc ccgggcgact gcccagtagg cgtgcagctc agcggtctcg
      181 caccgttcta agacctccgc caacacagca ccctccaacg ctcagtggat cgcctccaac
      241 cggaacgacc catcgatccc cgactccaac agcgacgcgt tgcgtcgctc atgggacagc
      301 tcagaaccct gatcagcacc atcggcgtcc gcgaaggcct cccacacctt gagcgccgcc
      361 acgaagtcgt catagcccag ggtcgtggcg tggcgggcga agatcgggtc cgcatcggcc
      421 acgtgctcgg ccccccgcgg gttcgagatg gtgcgggcga tggcccgcac gtgctcggtc
      481 gggatccgac cctccgcata cgcggcctcc acgagcggca ggcgccgcag ggcccggctg
      541 gtctgcaccc gaccgtgcgc ctcggacccc gacagccgcc cgatcgcctt caacgcgccc
      601 ttggccgtct tgtgcccgtc caagctgaac gctgcgctcg catccagatg cccggcggcc
      661 acatcggtgc cgtggtccaa ccggcggcgc agcacctcga gccgacgcac caaggccagc
      721 tcctcccgag gcgacagccc acccagatca gccgcctgca ccaggccaac cgcgtcctcc
      781 aacacggcca acgcacacgc gagaccgacc ttgggttcca tctggtcggt catggcgacg
      841 cctctcccga gacgtcaccg gaacatggtc ggtggtcgcc catgagcgac tccttccctt
      901 cgatggcgat ggtcggcgcg gagccgacga gtggcgctcc cagccccagc cggagcgcct
      961 acccgccatt caactcaggg ggtctgacag gaactcaccg catctctcgc atcgtcgttc
     1021 ctggaggcgc acgacggcgt cccggctcgc tgacaagatc agtccatgac gtctgtcgag
     1081 atcaccagct cgtggagcca gatcgggttc gtcctgttgt ccgcaggagc gatgctcacg
     1141 ggcatcatca tctacgtccg gatcatcggg ctccgcagct tctccaagat gtcgtcgttc
     1201 gacttcgcgg tgacggtggc gatgggttcg ctcctcgccg cggtgaccct ctcggggtcg
     1261 tcgctcgtcg acgggctcgt cggtgccggc gccctcctgg cggtccaggc tgcaatcgcg
     1321 tacgggcgga tccgcttcgg actcagcgcg gtcgtcgaca accaagcact gctgctcatg
     1381 gccgggccga cgatgctcga ggagaacatg cgccgggctc gggtgacgcc cgacgatctc
     1441 cgggcgaagc tgcgacaggc gaacatccac cgctacgacc aggtgcgcta cgtcgtgctc
     1501 gagaccacgg gcgacgtcag cgtggtccag ggagacggcg agctcgaccc ggacgtcctc
     1561 gccgatgtcg tcgacgccga gacgctgttc acccgctgag ctctctcaga tctccgtcgg
     1621 ggccccgccg gcggcggccg agcggtagac ggcatccacc agctcgtggg cccgcagcgc
     1681 gaccgcggcg tccggggtgg ccggtccgcc ctcggcgacg gcccggatga actcgccttc
     1741 ggggaggtcg gtggggattc cccggcgctg gcactcggcc atcagtccgt cacccgacca
     1801 ctcctgcgtg gcgccgtcca ccgtgtagcg caccggcccg aaccagtcct cctcgacgct
     1861 gatgaaggcc cgctcgcagt ggatctccag gtagcggagg ctgttgcggt cgggcaggtc
     1921 gtgccagacg gtggtcaggg tggccacgcc cccgccctcg agctcgaagg tggcgaccac
     1981 caggtcttcg atgccgtcga tctcgtggaa cgagcgctgg tggccgctca ccgaggtgac
     2041 cggaccgagc aggcggtcga tcaggtcgac atcgtggatc gagtgctcca gcagcacgcc
     2101 gctgccggcc ttgctggcat ccgcccgcca ggccgacgcg taccgcccgc cggtgggcag
     2161 cacctggtcg tcgcggaaca tggcgctcat gatccggccg ttgcgctcgg tgtcctcgag
     2221 gatcgactgc acgaggccgt acgccggcga gcgccggagg accagcccga cctggtgggt
     2281 ggtgccggcg agcaggtcgg tgacgcggcg ggcgccgtcg aggtcggtgc tgagcggctt
     2341 ctccacgaac acggcgaggc cggcgtcgac ggcgcggcgc acccgctcct cgtggtcgga
     2401 ggtccaggtg cagatgaaca ccgcgtcgca ggacccgagc acctcgtcct cggagtcggc
     2461 gacgaagccg cccgccgcgg cgacgaaggc ctcggctcgg tcgcggtcga ggtcgtgcac
     2521 gccggcgcgc tcgtgggcga cgccgctggc ccgcagcagc ttggagtgga aggtggcgat
     2581 gaagccggca ccgaggaagc cgacccgcac gagggacccg gccaccatca gttgttcttc
     2641 gtcgtgaagc cgccgtcgac cacgaccacg gcacccgtgc agtacgacat cgccggcagg
     2701 cagagcgaca gggtggcgtg ggcgacctcc tccggatcgc cgtagcgctt catcggcacg
     2761 ttgcggcgag cgaacttctg cttggcctcg tcggggatct cggaggtcat gccggtgtgg
     2821 atcggtcccg ggcagaccac gttcaccgtc accccggtcg ggccgagctc cacggccagc
     2881 gaccgggaca ggccgatcac gccgtgcttc gacgccgtgt acgggctgat gtaggcggat
     2941 ccgccgacgc cctcggtgga ggcgatgttc acgatgcgcc cgtcgccgtt gcgggtcagg
     3001 tgcggcaggc acgcacggat cgtcgtggtg tgcgcagtga ggttgaccgc cagcgtggtg
     3061 gcccactggg catcgaagtc cggcccgtcg atcggtgccg gcagcgagac gccggcgttg
     3121 ttcaccagcg catcgggcgg gccgagccgg tcgacgatct cgtccaccac ggcccgcacc
     3181 cggtcgccgt cggtgacgtc cacggcccag cccgctgccg tgccgccggc accggtgatc
     3241 tcgtccacga ccgcggcgac gccctcctcg gtgcggtcga gcaccgcgac cttggccccc
     3301 tcgtcggcga acaggtgcgc cgtcgcccgc cccatcccgc tcgccgcacc ggtgatcacc
     3361 gcgacccgcc cggccacgga gcggtcgccc ttcgtcaacc tcgtcatggc cgggaccata
     3421 accccacacg agaatggaaa cgcgtgtaga cagtgccgca ccggcatcgg ggggtgccgg
     3481 acggacaacg agccggcggt cgggggacca ccggtcaggg ggacgcatgc gtctggcgga
     3541 acggcggttg cccggggggc tcctcggacc acgagcgatg gcgtacgtgc caccggtcgc
     3601 catcctcgcg gtgcagcagg tgctgtaccc gctccccctc ggggtgtggg tgcgcggcgt
     3661 cctggtcggc ggcgtgaccg cgctgatcgc gctcggcatg gcgctgatct accggtcgaa
     3721 ccgcatcgtg aacttcgccc agggcgacct cggtgccgcc ccggcggtgc tcgtgttcct
     3781 gctcctcacc gagtggggtt ggccgtacac gctcgccgtg gcggccggcg ccctggcggc
     3841 gttcgcgctc ggcggggtcg tggagctcgc cgtggtgcgc cggttcttcc gggcgccccg
     3901 cctggtgctc accgtcgcca cgatcggcct cgcccagctg ctgaccggca tcgcggtgtt
     3961 cctgccccgt tggttcggca tcgagggatc gttcctggcg ccgcgggtgg aggacccctt
     4021 cgatgtccgc ttcgagctgg ccggcaccat cttccgcggc aacagcgtca tcgccttcgt
     4081 cgtcaccccg ctcctcgtgc tcggcctggt cgtgttcctc cgcgggacga aggtcggcat
     4141 cgccatccgc gcctcggcgg accgggccga ccgggccgcc ctgctcggcg tcccggtcaa
     4201 gcgcctccag accctggtgt gggccgtcgc cgcgctgctc gccttcgccg ccacgttcat
     4261 gcgagccggg atcctggggc tgccggtcgg ctccgtcctg agcctgggca tcctgctgcg
     4321 gtccctcacc gccctgctgc tcggcgggct cgtgcacctg ccggtcatcg ggctgtccgc
     4381 cgtcgccctc ggcgtcctcg agctcggcat cgactggcac cagggcggca ccgtcccgct
     4441 cctcggcatc gacctgccgc cgctggacgc cgtgctcgcc gtcgtggtcc tcatcgccct
     4501 ggtgctgcgg cgtcgcggcg acgggcgcag cgacatcgac gaccaatcct cgtggcaggc
     4561 ggccgacgag gtccgcccga tccccaccga gctggcccgc cttcccgagg tccgctggag
     4621 ccgggtcggc atcgccgccg tgctcgccgc ggtggccctg gccctccccc acgtcctcgc
     4681 cgtggaccag tccctgaagg cgtcggcggt catggtctac gccatcctcg ccatgtcgat
     4741 cgtggtgctc accggctggg cgggtcaggt ctcgctcggt caggtcgcgt tcttcgccat
     4801 cggcgccgcc accggggcca agctcaccca cacctgggac gtcgacctgc tcctggccgt
     4861 ggtcggcgcc ggcatcgccg gtgcggcagc tgccgtgctg gtcgggctcc cggcgctgcg
     4921 ctaccggggg ctctacctcg ccgtcaccac gttcgcgttc gccctcgcca ccaccgccta
     4981 cttcctcgac cgcggctact tcgactgggt gccgaaccag cggatcgagc gccagccgct
     5041 gctcgggcgc atcgacatct ccagccccac ccgcatctac tacgtcgtgc tcgccgggct
     5101 cgtgctggtg ctcgtcggcc tgcgcggcgt gcgccactcg cgcaccggcc gggtgctcat
     5161 cgccctgcgc gacaacgagc ggggggcaca ggcctacagc gtcgacgtgg tgcgggcgaa
     5221 gctcaccgcc ttcgccatct cgggcttcgt ggccgcgttc gccggcgccc tgttcgtgca
     5281 ccaccagcag gcgttcggca ccgggccgta ccagccggcg gagaacctgg ccgtgttcgc
     5341 catggccgtc gtgggcggca tcaccagcct gccgggcgcg ctgctcggcg ccctctacct
     5401 gctcggcacg cggtggttcc tccccctcga gtggcagttc ctggcgtcgg gcttcggcgt
     5461 cctgctcgtc ctcatgctcc tgccgagcgg cctcggcggg ctcctgttcg acctgcgcga
     5521 ccacgccctg cgctggatcg ccggccgtcg gggcatcctc gtcccgagcc tggtcgccga
     5581 ccgcgccgag ccccggaccg atgccgggga cgaccccacg ccgctgccct cggaggcccc
     5641 gacgcccgac gtggctgccc cggccgagga acccgaacga ccggtgaccg cgacgaccgg
     5701 gaggacgcag ccatgaggcg gttctggcag gtgctcgccc acccgaagca gtcgctgctg
     5761 tcgatcaccg acggggcgcc gatctacccc ctcatcgtgc tgttcggcct caacgccgtc
     5821 gacgagctgg accggaccgc cttcggcatc ctcgtgccgg agatccgcga cgagttcggc
     5881 ctcgacctgc agggcgtgct caccctcatc gcgttcgtct cgctctgcgc gctcacgctc
     5941 caggtgccga tcgcgatgct cgccgacaag tacaaccggg tgcgcatcgc ctggatcgga
     6001 gcggccgcgt gggccgtgtt cagcctcggc accggcttcg ccacgggcat cgtcttcctc
     6061 gccctcatgc gcgccggctc cggagtcggc cgcgccgtcg tggacccgac ccacaactcg
     6121 ctgatcgccg actactacga gcccggcatc cggccacgcg tcttctccgc acatcgcgcc
     6181 gccaacgccg tcggcgcctt catcggcccg ctcagcgccg ggctgctcgc cgcggcctac
     6241 ggctggcgga ccccgttctt cgtgttcgtc gtccccacgc tggtcttcgt cgtcctcgcc
     6301 tggcggctgc aaccaccggt gcggggcggg cacgagcgac gggcgatggg tgcctccgag
     6361 gaggccatcg ccaccgagga ggacgccccc tccttcgccg agggatggcg cctcgtctgg
     6421 aagatcgaga ccctccggcg catctggtac tcgctcccgt tcctggccgc ctccctgatc
     6481 ggcttcgtct cgctggcctc gctgctctac gacgaggtct tcgacctcga cgagcgcgcc
     6541 cgggggttcg tcgcggcgtc gaccgagccg atccagctgg tcggactcgt catcggtgcc
     6601 cgggtggcca cccgcctgct cgccgaggga cccgagaagg tgctggggtt cctggcccgc
     6661 acctcggtgc tcgtgtcggg gtgcctggtg gtcttcgcgc tggcgccgaa catctgggtg
     6721 gccatcgccg ccaacatggt catcaccggt gccctggccg tcatcggtcc gggcatcctc
     6781 gcctgcctgt cgctggccat cccaccccgg gcccgctcca tcggcttctc ggtggcgtcg
     6841 ctgtgggtga tccccggggt gctgctgctg ccgatcatcg gtgccgtcgg cgaccgctgg
     6901 gggatccgga ccggcatgct cctcatggtc ccgatgttcc tcatcggcgg catggtgatc
     6961 tccaccacga agcggaccat caccggcgac atcctgcagg tctggaaggc cgccgccgcc
     7021 cggtccgagg cgctcctcga gcgccgggcc ggccggtcca agctcctgtt gatccgcgac
     7081 gtcgaggtct cctacggcaa cgtccaggtg ctcttcggtg tcgacatgga ggtgggcgag
     7141 ggcgagatcg tggccctgct cggcacgaac ggcgccggca agtccaccct gctgaacgcc
     7201 atctccgggg tcaccgaggc ccaccgcggc gccatcatct tcgacggccg ggacatcacc
     7261 cacgccccgc cgaacgagat cgccgccatg ggcatcgccc aggtgcccgg cggcaagggc
     7321 gtcttcccct cgctcaccgt cggcgagaac ctcgagctgg ccgcctggct ccaccgcaag
     7381 gacggcgacc acggcggtct cgcccgcgtg cacgagctgt tcccgatcct cgaggcacgt
     7441 cgcgacgcgc cggccggtga cctcagcggc ggccagcagc agatgctcgc cctcgccatg
     7501 gcgttcctgt cgcgaccccg cctgctcatg atcgacgagc tcagcctcgg cctcgctccg
     7561 gtcatcgtca gccagctgct gccggtcgtc gaggagctgc ggtcccaggg caccacggtc
     7621 atcctcgtcg agcagtccgt gaacgtggcc ctcgacgtgg ccgagaaggc cttcttcctc
     7681 gagaagggcg aggtccgctt ctccggtccc accgccgacc tgctctcccg acccgacgtg
     7741 ctgcggtccg tgttcctggc cggccagctc ggcgcggcca ccggggacgg tgacggccgc
     7801 tcgccggctc ggacccagga gcgggtgccg atccccgacg acggcacgcc gccggcgctg
     7861 cagaccctcg acgtgacccg ctcgttcggc gggatcatgg ccgtcgacca cgtcagcatc
     7921 gaggtggcac cgaacgagat cctcggcatc atcggtccca acggcgccgg caagacgacg
     7981 ctgttcgatc tcctgagcgg cttcgtgccg gtcgactcgg gccgggtgct gctcggcgga
     8041 gccgacgtca ccgagcaggg cgccgccggt cgggcccgtc gcgggctcgg gcgctcgttc
     8101 caggacgccc ggctgttccc gtcgctcacc gtgcaggagg cgctggcggt gtccatggag
     8161 cggtgggtga cgagccgcga cccggtcagc gccgcgctgc acctgccgaa cgcgtacgac
     8221 gcggagcaga aggtggcgcg gcgggtggag gagctggtcg agctcctcgg cctcggcgcc
     8281 taccggtcca agttcatccg ggagctgtcc accggcacga ggcgcgtcgt ggacctggcc
     8341 ggcctgct
//
LOCUS       DATISU010000085         4707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_749008, whole genome shotgun sequence.
ACCESSION   DATISU010000085 DATISU010000000
VERSION     DATISU010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749008"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VLR27_03645"
     CDS             complement(<1..893)
                     /locus_tag="VLR27_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSP02566.1"
                     /translation="MADHAEGFDAPDLATWEAAIAKERGKPLDELTVTTPEGIEVKPL
                     YTEADLEGLSHIGGLPGQAPYVRGPYASMYTNRPWTLRQYAGFSTAEESNAFYRRNLA
                     AGQRGLSVAFDLATHRGYDSDHPRVVGDVGKAGVAIDSVEDAKILFDGIPLDQMSVSM
                     TMNGAVLPTMANYIVAAEEQGVSQDQLAGTIQNDILKEFMVRNTYIYPPAPSMRIVAD
                     IIGYTATHMPKFNSISISGYHMQEAGATLEQELAFTIADGMEYVRAAQDAGLDIDAFA
                     PRLSFFFCIGQNFFGEIAKLRA"
     gene            974..2065
                     /locus_tag="VLR27_03650"
     CDS             974..2065
                     /locus_tag="VLR27_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02567.1"
                     /translation="MMLRLCSIVVLALVLAACGDDDAAVRSEDVPGTSSGETEPTGSS
                     SAGGDLPFGLQRCPTEMTLVSAPEEWYRDEPVYIANEMPVEQVRAWAVDQPGFEDIWI
                     DRDHNGWLSVAFSEDAEARQADLEERFPGVGVVAVPVEWTEAELLALRDQAFGAMTDA
                     GFQPGGSHGVHTGLVEVWVGELIEEQLAPLAPFAGPRLCVTGVEPEDVIRPGPQPEAG
                     EGWRLLGTGRTGDSYRTGVATTDEQYASLWQQAGLGGDRPSVDFDEEVVIWFGAVYGS
                     GCEIRMDDVVFDLDRQLVHGDFVIPGVHQGCNDDANPEAYVVAVERSALPEAPFDVQL
                     DADDPPAGAPEERTTVTEDLRSPGATIPG"
     gene            2126..2878
                     /locus_tag="VLR27_03655"
     CDS             2126..2878
                     /locus_tag="VLR27_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HSP02568.1"
                     /translation="MLFEFDDVVVRFGEDVALDHVTAQVPDGGSVTCLLGASGSGKST
                     LLRLCNRLEVPTSGTVRFRGQPLDDLDPLQLRRTVGMVFQRPTPFPGTVRDNILVADP
                     EATEDAMRAVLEQVHLPASFLDREADSLSGGEQQRGSLARTLITRPEVLLMDEPTSAL
                     DPTATKALEELGRELADGGMPVLWVTHDLDQADRIGDRRIVLVRGRVADEHQAQHYLD
                     SEDAFHHHHGDETHEGHDDPTERDEGADRGAG"
     gene            2865..3647
                     /gene="fetB"
                     /locus_tag="VLR27_03660"
     CDS             2865..3647
                     /gene="fetB"
                     /locus_tag="VLR27_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HSP02569.1"
                     /translation="MEQGDVGWWGLAISLLLVAVAIGISMWRRLGLEQSMLVATGRAL
                     VQLLAVGYVLAIIIDPDQPIAYAWAWLAFMVPFAAWTVSRRAPEVPGVFGLALASLTA
                     STGITAAVIFGLGIFPVEARAIVPVGGMMVGNAMTSTVLAGRRVVEEFSEKRHDVEAR
                     LALGQPWTEASRPGLRSALRSAMTQQIETTKAVGLVFLPGAMTGLILAGVDPVDAVRV
                     QAAIMFLILGAVAINVVVIGTGVTRRLFTADHRLIRLARTSG"
     gene            3677..>4707
                     /locus_tag="VLR27_03665"
     CDS             3677..>4707
                     /locus_tag="VLR27_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSP02570.1"
                     /translation="MALSFVIIGGGPGGHTAASHAAKLGADVTLIEREIIGGAANLWD
                     CIPSKAMIATGSALNMIDKSEEMGLTDVKAHLDFGALRQRIVSIEDRLEHSVTRLLES
                     QGVRVLRGSGRMVGPHEVVADTAEGEISIHADAVLLATGSRPRIPEWAAPDGDRVLTT
                     RDAYPPKELPEHLIVIGSGVTGVEFVSMFSSFGCDVTLIVSRQQVLPQKDPEVAAALE
                     THFLDRGVRLYKGARAEGIERTDDGGVRILCDDGRVATGSHALLAIGSLPNSEELGCE
                     AAGVEINDWGYLDNNHHLQTNVPHIYVAGDLSGRLPLSSVASMQGRKIAEHVMGLHTR
                     EHRHLDYDKA"
BASE COUNT          658 a         1683 c         1674 g          692 t
ORIGIN      
        1 gcccgcagct tggcgatctc gccgaagaag ttctgcccga tgcagaagaa gaacgacagg
       61 cggggggcga aggcatcgat gtcgagaccg gcgtcctgcg cggcccgcac gtactccatg
      121 ccatcggcga tcgtgaacgc cagctcctgc tcgagcgtcg cccccgcctc ctgcatgtgg
      181 tagccggaga tcgagatcga gttgaacttc ggcatgtgcg tcgccgtgta gccgatgatg
      241 tcggcgacga tccgcatgga gggggccggc gggtagatgt aggtgttgcg gaccatgaac
      301 tccttgagga tgtcgttctg gatggtcccg gcgagctggt cctggctcac cccctgctcc
      361 tcggcggcga cgatgtagtt cgccatcgtc ggcagcacgg cgccgttcat cgtcatcgac
      421 accgacatct ggtcgagcgg gatgccgtcg aagaggatct tggcgtcctc gaccgagtcg
      481 atggccacgc ccgccttgcc gacgtcgccg acgacccggg ggtggtcgga gtcgtagccg
      541 cggtgggtgg cgaggtcgaa ggccaccgac aggccgcgct ggccggcggc gaggttgcgc
      601 cggtagaacg cgttggactc ctcggcggtc gagaagccgg cgtactggcg aagcgtccag
      661 ggccggttcg tgtacatcga ggcgtacggg ccccgcacgt agggcgcctg gcccggcagg
      721 ccgccgatgt ggctcaggcc ctcgaggtcg gcctcggtgt agaggggctt gacctcgatg
      781 ccctccggcg tggtgacggt cagctcgtcg aggggcttgc ctcgctcctt ggcgatcgcg
      841 gcctcccacg tggcgaggtc gggggcgtcg aaaccttcgg catgatcggc catggcccgg
      901 aggttacggc cctggtcacg gccgtaacca acggcgggaa ccagcggccc gggcccggcg
      961 tcagactggt gtggtgatgc tccggctgtg ctcgatcgtg gtgctcgcgc tggtcctcgc
     1021 ggcctgcggc gacgacgatg cggcggtgcg cagcgaagac gtcccgggga cgtcgagcgg
     1081 ggagaccgag cccaccgggt cgtcgtccgc tgggggcgac cttccgttcg gcctgcagcg
     1141 ctgcccgacg gagatgaccc tcgtgtcggc tccggaggag tggtaccggg acgagccggt
     1201 ctacatcgcg aacgagatgc cggtggagca ggtccgggcc tgggcggtgg accagcccgg
     1261 cttcgaggac atctggatcg accgggacca caacggctgg ctctccgttg cgttcagcga
     1321 ggacgccgag gcgcgccagg ccgacctcga ggagcggttc ccgggcgtcg gcgtggtcgc
     1381 cgtcccggtc gagtggaccg aggccgagct gctggcgctg cgcgaccagg ccttcggtgc
     1441 catgacggac gccgggttcc agcccggcgg gagccacggc gtccacaccg gcctggtcga
     1501 ggtgtgggtc ggcgagctca tcgaggagca gttggcgccg ctcgccccct tcgccggacc
     1561 gcggctctgc gtcaccggcg tggagcccga ggacgtgatc cgccccgggc cgcagccgga
     1621 ggccggggag gggtggcgcc tcctcggcac cggccggacc ggcgactcgt accgcaccgg
     1681 cgtcgccacg accgacgagc agtacgcctc gctgtggcag caggcgggcc tgggcggcga
     1741 tcggccgagc gtcgacttcg acgaggaggt cgtgatctgg ttcggcgccg tctacggctc
     1801 gggctgcgag atccgcatgg acgacgtcgt cttcgacctc gatcgtcagc tcgtgcacgg
     1861 cgacttcgtc atccccgggg tgcaccaggg ctgcaacgac gacgccaatc ccgaggccta
     1921 cgtcgtggcc gtcgagcggt cggccctgcc ggaggccccc ttcgacgtgc agctcgacgc
     1981 cgacgaccca ccggccggcg cccccgagga gcgcacgacg gtcaccgagg acctgcggtc
     2041 accgggcgcg acgatccccg gctgacgtca aggccgcggt gttccgtgac cgctgccaga
     2101 agccgctgcg ggcgatactg ccggggtgct gttcgagttc gacgacgtgg tggtgcgctt
     2161 cggcgaggac gtcgcgctcg accacgtcac ggcccaggtg cccgacggcg gatcggtgac
     2221 ctgcctcctc ggcgcctccg ggtccggcaa gtcgacgctg ctccgcctgt gcaaccggct
     2281 ggaggtgcct acgagcggga ccgtccgctt ccgggggcag ccgctcgacg acctcgaccc
     2341 gctgcagctg cgccgcaccg tcggcatggt gttccagcga cccacgccgt tccccgggac
     2401 ggtgcgggac aacatcctgg tggccgaccc cgaggcgacc gaggacgcca tgcgcgccgt
     2461 gctcgagcag gtgcacctcc cggcgagctt cctcgaccgc gaagctgatt cgctgtcagg
     2521 tggcgagcag caacgtggct ccctggcccg cacgctcatc acccgtcccg aggtgctgtt
     2581 gatggacgag cccacctccg cgctcgaccc caccgccacc aaggcgctcg aggagctcgg
     2641 gcgcgagctg gccgacggcg gcatgccggt gctgtgggtc acccacgacc tggaccaggc
     2701 cgatcggatc ggcgaccgcc ggatcgtcct cgtccggggc cgggtcgccg acgagcacca
     2761 ggcccagcac tacctcgact ccgaggacgc cttccaccac caccacggcg acgagaccca
     2821 cgagggccac gacgacccga ccgagcgcga cgagggagca gaccgtggag cagggtgacg
     2881 tcggctggtg gggcctggcc atctcgctgc tgctggtggc ggtggccatc ggcatctcga
     2941 tgtggcggcg gctgggcctc gagcagtcga tgctggtcgc caccggccgg gcgctcgtgc
     3001 agctcctggc ggtgggctac gtgctcgcca tcatcatcga cccggaccag ccgatcgcct
     3061 acgcgtgggc gtggctggcg ttcatggtcc cgttcgcggc gtggaccgtc tcccgtcggg
     3121 ctccggaggt ccccggcgtc ttcgggctgg cgctcgcgtc gctcaccgcg tcgacgggca
     3181 tcaccgccgc ggtgatcttc ggcctcggga tcttcccggt cgaggcccgg gccatcgtgc
     3241 cggtgggcgg gatgatggtc ggcaacgcca tgacgtcgac ggtgctcgcc ggccgacggg
     3301 tggtggagga gttctccgag aagcgccacg acgtcgaggc ccgcctcgcc ctcggccagc
     3361 cctggaccga ggcatcccgg cccggcctgc ggagcgcgct gcgctcggcc atgacccagc
     3421 agatcgagac gaccaaggcg gtcgggctgg tgttcctgcc cggcgccatg accggcctca
     3481 tcctcgccgg cgtcgacccc gtcgatgccg tgcgggtcca ggccgccatc atgttcctga
     3541 tcctcggggc ggtcgccatc aacgtggtgg tcatcggcac gggcgtcacc cggcggctct
     3601 tcaccgccga ccaccggctc atccgcctcg cccgcacctc cggctgatcc cgaggaccac
     3661 cggtaccctg cagaccatgg cgctcagctt cgtgatcatc ggtggtggcc cgggcgggca
     3721 cacggcggcc agccacgcgg ccaagctcgg agccgacgtc accctcatcg agcgggagat
     3781 catcggcggc gccgccaacc tgtgggactg catcccgtcg aaggcgatga tcgccaccgg
     3841 ctcggcgctg aacatgatcg acaagtccga ggagatgggc ctcaccgacg tcaaggccca
     3901 cctcgacttc ggcgccctcc gccagcgcat cgtgtccatc gaggaccgcc tcgagcactc
     3961 cgtcacccgg ctgctcgaga gccagggggt ccgggtcctg cggggcagcg gccgcatggt
     4021 cggtccccac gaggtcgtcg ccgacaccgc cgagggcgag atcagcatcc acgccgacgc
     4081 cgtgctgctc gccaccggct cccgcccccg catccccgag tgggccgccc ccgacggcga
     4141 ccgcgtgctc acgacgcgag acgcctaccc gcccaaggag ctccccgagc acctcatcgt
     4201 catcggctcg ggcgtcaccg gcgtggagtt cgtctcgatg ttctccagct tcggctgcga
     4261 cgtgaccctc atcgtctccc gccagcaggt cctgccccag aaggaccccg aggtcgcggc
     4321 cgcgctcgag acccacttcc tggaccgcgg ggtgcggctc tacaagggcg ctcgcgccga
     4381 gggcatcgag cgcaccgacg atggtggggt ccgcatcctc tgcgacgacg ggcgggtcgc
     4441 caccggcagc cacgccctgc tcgccatcgg gtcgctcccc aacagcgagg agctcggctg
     4501 cgaggccgcc ggcgtcgaga tcaacgactg gggctacctc gacaacaacc accacctgca
     4561 gacgaacgtg ccccacatct acgtggcggg cgacctgtcc ggccggctgc cgctgtcgtc
     4621 ggtggcgtcg atgcagggtc gcaagatcgc cgagcacgtg atgggcctgc acacccgcga
     4681 gcaccgccac ctcgactacg acaaggc
//
LOCUS       DATISU010000086         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_423434, whole genome shotgun sequence.
ACCESSION   DATISU010000086 DATISU010000000
VERSION     DATISU010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423434"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="VLR27_03670"
     CDS             <1..1239
                     /locus_tag="VLR27_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP02571.1"
                     /translation="MAEFSLQLNDDQIQIRDWIHEFAKDVLRPAGQEWDDKEEFPWPI
                     VQEAAKIGLYGFDFFANMSMADPTGLTMPIALEELFWGDAGIALSIFGSGLAAAGIAG
                     NGTPEQVMEWVPQCFGDENDVKIAAYCVSEPDAGSDVSSLRTRAEYDEAKDEWVLNGT
                     KAWATNGGIAHLHVVTATVDSTLGSRGQASFIIPEGTVGIEQGQKYKKHGIKASHTAE
                     VVLTDCRIPGSMLLGGKEKLDAKLAKAREGAKTGEKQPAMATFEATRPAVASMAVGIA
                     RAAYEIALEYAHEREAFGVPIFENQAIAFMLADMKTEIDASRLLVWRAAWLSRNGGGY
                     QYAEGSMSKLKAGRTATWVTERAMQILGGYGYTREYDVERMHRDAKIMDIFEGTEQIQ
                     QGIVARAISEGRIDPLRQNR"
     gene            1461..1892
                     /locus_tag="VLR27_03675"
     CDS             1461..1892
                     /locus_tag="VLR27_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSP02572.1"
                     /translation="MRVTELMHVPPVTCEVSATLGDVAGVMRERNVGSVLVVDNIGYL
                     AGIVTDRDITIRGMAGGRTADSKVEHVMSRDVATIGLHADVSDAASVMAKRMVRRLPV
                     VDEEDRPHGMVTFDDMVRHLGTETDALADLVILQASRLAGT"
     gene            complement(1927..>2333)
                     /locus_tag="VLR27_03680"
     CDS             complement(1927..>2333)
                     /locus_tag="VLR27_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HSP02573.1"
                     /translation="GTMAAFFVSCAAAGWSPGQDFPTGPALAAASGAAFMTVVVAQTA
                     NAFACRSATLSVAAMHPLGNRLLPAASAVELGVSLVMLLWEPAARALEHASPPLEGWL
                     VAGATAAVLLGADAVDKAHRRRTQLTRASGPP"
BASE COUNT          395 a          824 c          781 g          333 t
ORIGIN      
        1 atggccgagt tctcgctcca gctcaacgac gaccagatcc agatcaggga ctggatccac
       61 gagttcgcca aggacgtgct gcgtccggcg ggccaggagt gggacgacaa ggaggagttc
      121 ccctggccga tcgtgcagga ggcggccaag atcggcctgt acggcttcga cttcttcgcc
      181 aacatgtcga tggccgaccc caccgggctc accatgccca tcgccctcga ggagctgttc
      241 tggggtgacg ccggcatcgc gctgtccatc ttcggctccg gcctcgccgc cgccggcatc
      301 gccggcaacg gcacccccga gcaggtcatg gagtgggtgc cgcagtgctt cggtgacgag
      361 aacgacgtga agatcgcggc gtactgcgtc tccgagcccg acgccggctc cgacgtcagc
      421 tccctgcgga cccgcgccga gtacgacgag gccaaggacg aatgggtcct caacggcacc
      481 aaggcctggg ccacgaacgg cggcatcgcc cacctccacg tggtcaccgc aacggtcgac
      541 tccacgctcg ggtcgcgcgg gcaggcgagc ttcatcatcc ccgagggcac ggtcggcatc
      601 gagcagggcc agaagtacaa gaagcacggc atcaaggcga gccacacggc cgaggtcgtc
      661 ctcaccgact gccgcatccc cggctccatg ctgctcggcg gcaaggagaa gctggacgcc
      721 aagctggcca aggcccgcga gggcgccaag accggcgaga agcagccggc catggccacc
      781 ttcgaggcca cccgtcccgc cgtggcctcg atggccgtcg gcatcgcccg agccgcctac
      841 gagatcgcgc tcgagtacgc ccacgagcgc gaggcgttcg gtgtgcccat cttcgagaac
      901 caggcgatcg ccttcatgct cgccgacatg aagaccgaga tcgacgcctc ccgcctcctc
      961 gtgtggcggg cggcgtggct gtcccgcaac ggcggcggct accagtacgc cgagggctcc
     1021 atgtccaagc tcaaggccgg gcgcaccgcg acctgggtca ccgagcgggc catgcagatc
     1081 ctcggtggct acggctacac ccgtgagtac gacgtggagc gcatgcaccg cgacgccaag
     1141 atcatggaca tcttcgaggg caccgagcag atccagcagg gcatcgtcgc ccgggccatc
     1201 tccgagggtc gcatcgaccc gctccgccag aaccgctaga cggtctgttc tgcaggccct
     1261 gcctgcatcc aggccatcag caccctgttc tcgagggctc cggcttcccc ctgccggagc
     1321 cctcgacgcg tcccggggta cctgagcgac cagtcccgcg acgcctcagc gggggctcca
     1381 ggggaccttc tgctctacat cgaccccacc agtcgtgctt cacatggagt catccccgcc
     1441 gccagaccaa ggagcccacg atgcgagtga ccgaactgat gcacgtcccg cccgtcacct
     1501 gtgaggtgtc cgcaacgctc ggagacgtcg ctggcgtcat gcgcgagcgc aacgtcggca
     1561 gcgtcctggt ggtcgacaac atcggctacc tggccggcat cgtcaccgac cgcgacatca
     1621 ccattcgggg gatggccgga ggtcgcaccg ccgactccaa ggtcgaacac gtcatgtctc
     1681 gagatgtcgc aacgatcggg ttgcatgctg atgtgtcaga tgctgcatcg gtcatggcga
     1741 agcggatggt ccgacggctt ccggtggtcg acgaggagga ccggccgcac ggcatggtga
     1801 ccttcgacga catggtgcgc catctcggca ccgagaccga cgcgctcgcc gacctcgtca
     1861 tcttgcaggc gagtcggctc gccggaacct gacccgcgag catcgagcca gccgggacgc
     1921 tgcggatcat ggtggtccgc tcgcccgtgt gagctgcgtc ctccggcggt gggccttgtc
     1981 gactgcgtcg gccccgagga ggacggcagc ggtcgctccg gccaccagcc agccctcgag
     2041 gggtggcgat gcgtgctcca gcgcccgggc ggccggctcc cagagcagca tgaccagcga
     2101 gacgcccagc tcgacggccg atgccgctgg gaggagacgg tttccgaggg ggtgcatggc
     2161 cgcgacgctg agcgtcgcgc tccggcaggc gaatgcgttg gccgtctggg cgacgaccac
     2221 ggtcatgaac gctgctcccg aggccgcggc gagggcgggt ccggtcggga agtcctgccc
     2281 gggtgaccag ccggcggccg cgcacgacac gaagaaggcc gccatggtcc cga
//
LOCUS       DATISU010000087         2834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_656168, whole genome shotgun sequence.
ACCESSION   DATISU010000087 DATISU010000000
VERSION     DATISU010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656168"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VLR27_03685"
     CDS             <1..448
                     /locus_tag="VLR27_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02574.1"
                     /translation="GMCDHCSCRQHRAIAELSTEHEQILEVAWALSERHRETGVSDGP
                     LQEQLGQMLAIHVEAEEVALYPLLVETGGLQPDHSDALEQEHTDLAAALISGKFDRRM
                     YFELASHIEEEELELFPLAMFGFDDDDWEVLEATPRFLAPDTPLVR"
     gene            complement(468..1034)
                     /locus_tag="VLR27_03690"
     CDS             complement(468..1034)
                     /locus_tag="VLR27_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HSP02575.1"
                     /translation="MDDRAVELFVESIGRFADRVDDITDDQWSRPTPCAAWDVRTLVD
                     HVCNEQLWAPHLVDGETIEQVGDRYDGDVLGDDPKATFRRAVEASTAAFQRADLDAIV
                     HLSFGDVPCRIYLDQMLTDAEVHGWDLATGLGDDAELEPETASYLLPGMQAQEELIRS
                     SGMFGEAVPVDDGADDATRLLALLGRRP"
     gene            1144..2082
                     /locus_tag="VLR27_03695"
     CDS             1144..2082
                     /locus_tag="VLR27_03695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HSP02576.1"
                     /translation="MSHPRPFRFGVQLANATSASAWADLARRAEALGYDSVMMPDHFG
                     EQLAPVPALMAAADATTELKVGTLVFATDYKHPVVLAKEVATLDVLSEGRVEFGIGAG
                     WMNTDYEQSGIPHDRAGVRIDRLAESLEICKAIFAGDTVDFTGEHYDVTGYQGYPKPV
                     QTPHPPILIGGGGKRILTLAAQHADIVGINPKIASGKVDTPTAQNGAADRTDEKLGWV
                     REAAGDRYGDIELNMLVFAVVVTDDAQGTMEMLAPMFGLDPAEVADYPLAWIGTEDEI
                     VESLQARRERWDVSYLVGQQDAMETAAPIVARLRGT"
     gene            2290..2610
                     /locus_tag="VLR27_03700"
     CDS             2290..2610
                     /locus_tag="VLR27_03700"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HSP02577.1"
                     /translation="MPRYLVEVSYKPEGLQGLLAKGGTARREMIEKMLAEVGGKVESF
                     DYAFGANDAYLIVDVPDHATVAAITMMVGATGTVACKTTVLLSPEEIDRAAAVKASYT
                     PPGA"
BASE COUNT          444 a         1031 c          988 g          371 t
ORIGIN      
        1 tggcatgtgc gaccactgca gctgccgcca gcaccgggcc atcgccgagc tctcgaccga
       61 gcacgagcag atcctggagg tggcgtgggc actgagcgag cgccaccgcg agaccggcgt
      121 gtccgacggc ccgctccagg agcagctcgg gcagatgctc gcgatccacg tcgaggccga
      181 ggaggtggcg ctgtacccgc tcctcgtcga gaccggtggg ctccagccgg accactccga
      241 cgccctcgag caggagcaca ccgacctcgc cgccgcgctg atcagcggga agttcgaccg
      301 gaggatgtac ttcgagctgg catcccacat cgaggaggag gagctcgagc tcttcccgct
      361 cgccatgttc ggcttcgacg acgacgactg ggaggtgctg gaggcgacgc cgcgcttcct
      421 ggcaccggac acgccgctcg tccgctgacg ggcgcgcctc ccgtcggtca gggccggcgg
      481 cccagcaggg cgagcagccg ggtggcgtcg tcggcgccgt cgtccacggg gacggcctcg
      541 ccgaacatgc ccgaggaccg gatcagctcc tcctgggcct gcatcccggg cagcaggtac
      601 gacgccgtct ccggctcgag ctcggcgtcg tcgccgaggc ccgtcgccag gtcccacccg
      661 tggacctcgg cgtcggtgag catctggtcg aggtagatcc ggcacggcac gtcgccgaag
      721 gacaggtgca cgatggcatc gagatcggct cgctggaacg cagcggtcga cgcctccacc
      781 gcccgccgga acgtggcctt cgggtcgtca ccgaggacgt cgccgtcgta gcggtcgccg
      841 acctgctcga tcgtctcgcc gtcgacgagg tgcggcgccc acagctgctc gttgcagacg
      901 tggtccacca gggtccgcac gtcccatgcc gcgcagggcg tgggacggct ccactggtcg
      961 tcggtgatgt cgtcgacccg atcggcgaag cgcccgatcg actcgacgaa gagctccacg
     1021 gcgcggtcgt ccatggacga atcatgcacc ggggaccgcc cttcggccac cggaccccgg
     1081 ggagggcccc ggccggtgtc gggttaggtt cgcccggacc gcctgccgat cccaggagcc
     1141 cccgtgagcc acccccgtcc cttccgcttc ggcgtccagc tggccaacgc cacctccgca
     1201 tcggcgtggg cggacctcgc ccggcgagcc gaggcgctcg ggtacgacag cgtgatgatg
     1261 ccggaccact tcggcgagca gctcgccccg gtcccggccc tcatggccgc cgccgacgcc
     1321 accaccgagc tcaaggtcgg gaccctcgtc ttcgccaccg actacaagca ccccgtcgtg
     1381 ctggcgaagg aggtggccac cctcgacgtc ctgtccgagg gccgggtcga gttcggcatc
     1441 ggcgccgggt ggatgaacac cgactacgag cagtcgggca tcccccacga ccgggccggc
     1501 gtgcgcatcg accgcctcgc cgagagcctg gagatctgca aggcgatctt cgccggcgac
     1561 accgtcgact tcaccggcga gcactacgac gtgacgggct accaggggta cccgaagccg
     1621 gtgcagaccc cgcacccgcc gatcctcatc gggggcggcg gcaagcggat cctcaccctc
     1681 gccgcccagc acgccgacat cgtgggcatc aaccccaaga tcgcctcggg caaggtcgac
     1741 acgcccacgg cgcagaacgg tgccgccgac cgcaccgacg agaagctggg gtgggtccgg
     1801 gaggcagccg gcgaccgcta cggcgacatc gagctgaaca tgctcgtgtt cgccgtcgtg
     1861 gtcaccgacg acgcccaggg gacgatggag atgctggccc ccatgttcgg cctcgacccc
     1921 gccgaggtgg ccgactaccc gctggcgtgg atcggcaccg aggacgagat cgtcgagtcg
     1981 ctccaggccc ggcgcgagcg ctgggacgtc agctacctgg tggggcagca ggacgccatg
     2041 gagaccgccg cgcccatcgt cgctcggctg cgcggcacct gagccggacc gccggccgcg
     2101 ctccggcgtc gcgcgcgtcg cgctcggcgt ccgcacgccc gctgtgtgtg cgctccggca
     2161 cgagcccggg gtccggtgcg acgccgagcg gtcggcgcag cgacctcgat ggtggaatcg
     2221 tgcctttgcc ctgcttgcgg gtctgacccg cggggcagag ggtcagcggt gagccaaggg
     2281 aggccccaca tgccgagata cctcgtcgag gtcagctaca agccagaggg actgcagggc
     2341 ctgctcgcca agggcggcac cgcccggcgg gagatgatcg aaaagatgct cgctgaggtc
     2401 ggcggcaagg tggagagctt cgactacgcg ttcggcgcca acgacgcgta cttgatcgtc
     2461 gacgtgcccg atcacgccac cgtcgccgcc atcacgatga tggtcggcgc caccggcacg
     2521 gtcgcgtgca agacgaccgt gctgctcagc cccgaggaga tcgaccgggc cgccgccgtg
     2581 aaggcgagct acacgccgcc cggggcctag gcccccgagc cgtgacgttg gaccctggtc
     2641 gctcgtccca cccgggcgga cgatcgaggc aacgtcacgg acaacgggga ggaagcgcca
     2701 tgcgcgccat cgtgtaccac gggccgggca agaaggactg ggaagaggtc ccgaagccgg
     2761 ccatccagga cgacaccgac gccatcgtgc gcgtcgacgc cgtcaccatc tgcggcacgg
     2821 acctgcacat cctg
//
LOCUS       DATISU010000088        28424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_50921, whole genome shotgun sequence.
ACCESSION   DATISU010000088 DATISU010000000
VERSION     DATISU010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 28424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28424
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50921"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /gene="ftsH"
                     /locus_tag="VLR27_03705"
     CDS             complement(<1..1216)
                     /gene="ftsH"
                     /locus_tag="VLR27_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HSP02578.1"
                     /translation="MKKKLRRPTLIYVALALVAALVASSFLRSGSDPEQFTLNEFRDY
                     VADGEVETATIKDRSHDVQGELRDGTEYKVSYPAEFADELTIELSEAEPAIEIETDQQ
                     QESIWVSLLFSILPLLLLFGLFLFFLNAMQGGGSRVMQFGKAKAKQWNKDQPKVTFDD
                     VAGCDEAVEELHEIKEFLQSPAKFQAIGAKIPKGVLLVGPPGTGKTLLARAVAGEAGA
                     PFFSISGSDFVEMFVGVGASRVRDLFEQAKAQAPAIVFVDEIDAVGRHRGAGMGGGHD
                     EREQTLNQLLVEMDGFDANTGVILIAATNRPDILDPALLRPGRFDRQIVVDRPDLEGR
                     KAILDVHAKGKPLADDVDAAVIARRTPGFTGADLANLMNEAALMAARRSVDTIDMHTV
                     ESAIDRVLAGPER"
     gene            complement(1266..1808)
                     /gene="hpt"
                     /locus_tag="VLR27_03710"
     CDS             complement(1266..1808)
                     /gene="hpt"
                     /locus_tag="VLR27_03710"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HSP02579.1"
                     /translation="MAAQGDDLIGDVLLSEAQIQERITELGAEIARDYAGKDPILVAV
                     LKGAFIFMADLARAVDIPLEVDFMAVSSYGASTRSSGVVRIVKDLDIDLHDRHVIIVE
                     DIVDSGLTLRYLRKTFESRGTASLEVCALLVREGNQQAELDLRYIGFRIPPAFVIGYG
                     LDVAERYRNLPHIADYRGDE"
     gene            complement(1820..2707)
                     /gene="tilS"
                     /locus_tag="VLR27_03715"
     CDS             complement(1820..2707)
                     /gene="tilS"
                     /locus_tag="VLR27_03715"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HSP02580.1"
                     /translation="MSATLDAVRGRCRFPSRGPVTCAVSGGPDSLALLVLACDAGLDV
                     TAVHVDHGLRAGSEAEADVVAGAASRFGAAFRAERAEVHPGGDLEARARSARRRLLGP
                     DALYGHTADDQAETVLLALLRGSGLDGVAEMRAEHHPLLALRRSETHALCAELGLEPV
                     SDPTNHDPRFRRNRVRAEVLPLLDDVAERDVVPLLCRFAELARADVDLLDQLASALDA
                     TDAPALAAAPPPLAGRAVRRWLRALLPGGHPPDAAAVQRVLAVARGEGVGTEVAGGVR
                     VRRRAQRLTADGSAGRGDR"
     gene            complement(2713..3465)
                     /locus_tag="VLR27_03720"
     CDS             complement(2713..3465)
                     /locus_tag="VLR27_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSP02581.1"
                     /translation="MASVLVVDDEPDIRYLTQLQLELDGHRVMTAADGAEALAAVQVE
                     VPDVVLLDVMMPGVDGWSVLEQLKAHLDEQISSVKVLMMTALGTDDDRLRGGIEGAVR
                     YLVKPVSADQLRAAIADVMAGGSERDQRRKAQTGALSELARIQRGGDADAPRDERAGP
                     RLSRLEHARPRDEHHVDDGAVHVGGDLTEKQRSLLRTLAAAPSVSAAAGDLGVSRSNI
                     YASLRRIIRKTGHESVPELLRRLRAGQVVVDG"
     gene            complement(3545..4636)
                     /locus_tag="VLR27_03725"
     CDS             complement(3545..4636)
                     /locus_tag="VLR27_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HSP02582.1"
                     /translation="MAELVDWDLAERIAVRAAGREPLADSYHWESLEPDFAELTALAE
                     ELVAAETGLHSLAGPARGKVTDRAGWVRANISSFQRLLGPFTEKMAPRVSGPTVPIMR
                     AAAAAEVGLLLGWMSGRVLGQYDLLVAGGSDDDTDGQPADADLVYYVGPNVLALEKKF
                     GFPPREFRLWLALHEVTHRAQFTGVPWMRDHYLGLIDRTLSAAGLDPMVLLRALKHTA
                     EELRAGRSPLPEGGLAHLLAGPEQRAALDEIGGLMSLLEGHGDVTMDRAGEGHVPSAE
                     RFGRVLRNRRASATGLTKLVQRLIGLEAKLAQYQQGEDFIAEVERLGGGKGALAPVWR
                     GPSWLPTLTEIREPHEWVERVRLSEELVT"
     gene            complement(4629..4772)
                     /locus_tag="VLR27_03730"
     CDS             complement(4629..4772)
                     /locus_tag="VLR27_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02583.1"
                     /translation="MLAIIWHWWIAVVLMITLVVPAVVMTVVLYLRKVVKPQYPGVDQ
                     PRG"
     gene            complement(4818..5060)
                     /gene="rsrA"
                     /locus_tag="VLR27_03735"
     CDS             complement(4818..5060)
                     /gene="rsrA"
                     /locus_tag="VLR27_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HSP02584.1"
                     /translation="MDHGDCSETLHELYHFLDGELTDAKRVAIQEHLEECPPCFEAFD
                     FEAEIKQYIAQKCRDRVPESLRSRIADAIGHEPPAG"
     gene            complement(5080..5685)
                     /locus_tag="VLR27_03740"
     CDS             complement(5080..5685)
                     /locus_tag="VLR27_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP02585.1"
                     /translation="MADQATFAAQAMPFMDSLYGAALRMTRNQSDAEDLVQETFLKAY
                     RGFGGFQEGTNLKAWLYRILTNTYINIYRSKKRRPDETDLAEVEDLYLYRRLGGLEGA
                     TAGRSAEDELLELFSEAEVKDALESLPETFRMAVLLADVEGFAYKEIAEILDIPIGTV
                     MSRLHRGRKALQKSLYEFAVARGLTERTDDAPDESQPANTG"
     gene            complement(5730..6410)
                     /locus_tag="VLR27_03745"
     CDS             complement(5730..6410)
                     /locus_tag="VLR27_03745"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HSP02586.1"
                     /translation="MERRRSVTAAAALLLLLLAGVACGAPAGDGASEDGVVASPTAPT
                     TTTPETSVPEPRPTSALAERVQPLDSARYDPDEHQAATRTVTGLSLPAIGVEGAPVEP
                     VGVEPNGEMEIPPADEVGWYRFGASPGEPGSAVLAAHIAYDGVDGVFRRLDRLGAGDE
                     VAVAFDDGSREAYLVTEVVTFDKDELPDDLFARDVPERLVLITCGGEFNPELRSYESN
                     VVAYAVPA"
     gene            complement(6412..7218)
                     /locus_tag="VLR27_03750"
     CDS             complement(6412..7218)
                     /locus_tag="VLR27_03750"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HSP02587.1"
                     /translation="MRKIPTIIGVLAAAVLVVLATPSAAQESARIHLIHGIPDTPVDV
                     VAGGEVVFSDFQFGDTQDLSGLAGQTLTGLTVNAAGTDTVAIDAGDTALPASGNYTII
                     AHLDAEGTPTLGVFENDTSATAAGEGRLTVRHTAAAPAVDVLANGEAAFTNLSNPNEA
                     SADLPAGTISASVVPAGAADPVVIGPADLAVTDGTSLIVYAVGSLDADSLTVLTESIE
                     GLGTAPTAVNTGNSPVSDDAGLPVGLLALAGVALLATAVPARRLVAARVR"
     gene            complement(7294..9582)
                     /locus_tag="VLR27_03755"
     CDS             complement(7294..9582)
                     /locus_tag="VLR27_03755"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HSP02588.1"
                     /translation="MRTRHAHRGIRTFAVLAAVTVAATACSSDSGSPPSSGGQAFQGL
                     RGAAPVALQSDRSCGDVLDDFQSFAPAVLSDQMFGFAEGGAATTSAEFEDVGDDMSAD
                     AGAERAAAPDEASAQLEAADGSSSDTNTQEAGIDEPDVVENDGEFVYVVDHGPDQAHL
                     VILDGATAEVLSRTPLASYGAQLLLQGDRMLVVTSGGYGYPMPMPMPVEDGAAVSGPA
                     PDEGLDASEPVEPTETTVPPGQVDPPEPTDPEEPTEPSEPAEPSVPVEPTETTLAPEP
                     VEPIEPLPEPEPLPEPIPVDPFPMPTEPPSFAAGTVLQLLDVSDRRSPQIVQTTEIEG
                     HHVSTRVVDGVARVVVSSYPDAQPLLEDASMRLGDGSDQDSARAAMEAGIADTTIDDW
                     LPAFRTTVADAGEVASRTSTEGSLVACEDVFVPEVNAGIAQTSVLRVDFTDGFDPADT
                     TTVVAEAGTVYASTSTLYVAANTYVPLQEQGRIVGEDFSTALHAFDLRGDGPAAHLGA
                     GEIPGHLLNQYSLSEHDGYLRAATTEGAPWGGGEDSQSGVRVLQLDGGALVEVGAVTG
                     LGVTETIQSVRFMGPVGYVVTFRQTDPLYVIDLSDPRAPRAVGELKIPGFSSYLHPVG
                     EGRLVGIGRDADLEGRDQGLLVSLFDVADPTAPTQLQTWTERDAWSIAGDDPKAFLHW
                     APESAVIVPLERFGSGSVQSGIVVLDVADSGITERALVTADGRYPSRALVVQGKLWSL
                     FDGGVVVSEFANPAQGAFTGFR"
     gene            complement(9627..10925)
                     /locus_tag="VLR27_03760"
     CDS             complement(9627..10925)
                     /locus_tag="VLR27_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HSP02589.1"
                     /translation="MITTVVLPIIAVLVLVLVNGLFVAAEFALVGARRSRVQAMADSG
                     STAARWLLGVFDRPTGKDGYIAIAQLGITLASIGLGMYGEPAVAGWLYKPLEDGLGLS
                     YGASHAVGFAIALSAITFLHVVFGEMIPKALALQAPESVVLRVNPAMRAFGLVFRPMV
                     AVLNAAALGLMRLLRIPEPDKSTALYTSAELEIVTDEAASSGQLGALQRDLIRNIFEL
                     DERRAEELMTSRGRLEVLDLAASADEVAAQVTASPRSRYPVIDGDLDHVVGVLHIKDF
                     IRASRRSTVVLADLIRPLPTVAATASAEQLLERFTRTRTHACLVVDEVGGTLGVVTLD
                     DVIAEVMEEGDESGSSAAVHHDDGSISLDGEVTLAELRDDHGLTFTHDEVATLAGLVL
                     AAHGTVPDVGTAVDVQGHELVVEDVLGHKITRVRVRRLER"
     gene            complement(10922..12214)
                     /locus_tag="VLR27_03765"
     CDS             complement(10922..12214)
                     /locus_tag="VLR27_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HSP02590.1"
                     /translation="MSIVIPLLLIAVMVSFNALYVAAEFATVGSRRSRVQEDAAGGSA
                     AAGRLLEILEDPKRLDNYVAACQIGITLSSLVAGAYGQAALTPLLEPALGSVGGRAVA
                     VLIVLVGVTSVQVVLGELLPKTAALRYPEKLAMLTLPPMAISQWLFRPLVAIFNGSAF
                     RLMKLWGLAVDHSHAHVHSPEELAGLYRASAEGGLIDASERDMLAGVLNVEDRLVREI
                     MTPRRQLTTVPATLGVDDALARLADSAHTRFPVTGSDDDIVGIVHLRDLWVAARQDGS
                     RSVGDLARPVPAVAEVLTVPGLWDELRDEEEHLALVVNEFGSVAGLVTMEDAIEEIFG
                     EVLDEFDVEEDPITVDQRRVSVRGDVLVEVLEDRFGTTLTEDDVDTIGGLVWHELGRR
                     PREGDEVPVGPDGVVLRVDRVDGTAVARASFDVPGGAA"
     gene            complement(12211..13512)
                     /locus_tag="VLR27_03770"
     CDS             complement(12211..13512)
                     /locus_tag="VLR27_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HSP02591.1"
                     /translation="MRDVLPELGLVAVLVLLNAAFAGTELALVSLREGQLQRLERQSS
                     TGAVLADLARDPNRFLATIQIGITLAGFLASAAAAVSLAEPLEEPLGFLGRASEPVAV
                     VVVTLLLAYVTLVFGELAPKRVAMQRAERWGMVTARPLAFISVLARPVVWLLSHSTDI
                     AVRLMGGDPHLDREEITEEELRDMVAVQTSFTPKQRLIIDGAFEISERTLEEVLRPRG
                     EVFVLDAAQPGADALEGLAASGHSRAPVGVNGNLDEVIGVVHLRDLLGLGSRPVGEVV
                     SEIRAFPETAGVLDALHQMQQSHAQLAVVVDEHGSGAGIVTVEDLVEELVGEIYDETD
                     RDILSVQHGDDGSMLLPGQFPVHDLTDVGIDGVEDGPYTTVAGLILDRLGRVPETPGD
                     RVPLDDWVLEVDTVHRHAITQVRAIPRGPDDVTAHPSEETR"
     gene            complement(13598..13933)
                     /locus_tag="VLR27_03775"
     CDS             complement(13598..13933)
                     /locus_tag="VLR27_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02592.1"
                     /translation="MQSDDPPPQPATIRARSLVLAVLALVGVLLAVAGADTTVTDASG
                     AAPIVDDAARQDPVQYEQGAHEVADSGPSSGVEGRLDARPHRCSEITEDPRIDRGCSP
                     PTLRGPPGA"
     gene            complement(13942..14014)
                     /locus_tag="VLR27_03780"
     tRNA            complement(13942..14014)
                     /locus_tag="VLR27_03780"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13979..13981),aa:Thr,seq:cgt)
     gene            complement(14058..15167)
                     /locus_tag="VLR27_03785"
     CDS             complement(14058..15167)
                     /locus_tag="VLR27_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP02593.1"
                     /translation="MTDTADLGAALAPADVEALVERADLWHDVVGQGRAVAALQAAAT
                     RPVHAYLLVGPAGSGKRAAARAFAAALLAIDAAGRGADVERAIELCLDESHPDLQVFE
                     PEGAALTMGDAEDIVTAASRSPVEGRRKVLVLEEFHKVQQAGPALLKTIEEPPASTVF
                     VILAEEVPNELVAIASRSVRIDLGPVPQDAVTDRLVAEGIDREVAATTAAAAGGDLHR
                     ARLLARDPSLVVRRDSWASIPHRVDRTGHTAAALVDEVVAHIDAAQGALDERHEAERL
                     ALEEQIEAYGMRTTPLKDLEKKHKREVRRHRNAEIRFGLAVLAGEYRDRLATAGDPTP
                     HLAALRAVQEAAEALVRNPNETLLLQAMVGGLDPL"
     gene            complement(15320..15934)
                     /gene="tmk"
                     /locus_tag="VLR27_03790"
     CDS             complement(15320..15934)
                     /gene="tmk"
                     /locus_tag="VLR27_03790"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HSP02594.1"
                     /translation="MTARFLAFEGGEAAGKSTQTERLARRLDALLTREPGGTPLGAAI
                     RGIFLDPATGELDPRTEALLMAADRAQHVATVIRPALAAGRHVVTDRYLHSSIAYQGH
                     GRGLDPAGVERLNRWATDELLPDVVVLLDVPVDVARQRIAHQAPDRLESEADGFHERV
                     RDGFLAQAASHPDRWVVIDATGDPDDIAAAVWEAVTARLPDLDP"
     gene            complement(15931..17469)
                     /locus_tag="VLR27_03795"
     CDS             complement(15931..17469)
                     /locus_tag="VLR27_03795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP02595.1"
                     /translation="MAEPSEARPTTPRPGDGPVGTPDTQAEVPTLAMEALAEGEPRGH
                     VRLFGSTAFFRLWLTQVVSATGDWLGFLAIAALATRISDRPEAAVGLVMSARIVPGFF
                     LGPAAGVFADRFDRKRLMVMCDVGRALVLASLPFVDTVPGLIVASLVMEVFTLLWAPA
                     KEASVPHLVPSDHLTTANSLSLAAAYGTIPIAAGLFSLLAELSDTIGDFELLTVVRGT
                     QEGLAFYFDAGTFLLSALMIWSLPLPRRRKADRARGAKRIEFGAAWRELREGWQFVFL
                     NPVVRAVNLGLATGLIGGGMLVPLGPVFADQVLGAGQSGFGVLIFALGLGVAGGVITI
                     SVFQRRVPKEWVFVVAVFVAGFSLGTAASMSALELAAAFVFVLGICAGCVYVLGFTML
                     HEHVEDELRGRIFSALYTLVRLCVLIAFAVGPFLSELLGELSESLFDDGHVGLFGVSI
                     AVPGVRLTLWTASAIIVVAGFIAAHSMLDAARKELVTGPPAVHRGGTARADATPTGDD
                     QVDG"
     gene            complement(17524..20211)
                     /gene="topA"
                     /locus_tag="VLR27_03800"
     CDS             complement(17524..20211)
                     /gene="topA"
                     /locus_tag="VLR27_03800"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HSP02596.1"
                     /translation="MSKPLVIVESPAKAKTISQFLGNDYIVEASVGHVRDLPSKGLSI
                     DVDADFQTEYEVNPNKKDVIKRLKALLKDADELYLATDEDREGEAIAWHLKEVLKPKV
                     PVKRMVFHEITRSAIEHAVENSRDIDYRRVDAQETRRIVDRLYGYPVSQIMWRKVNRG
                     LSAGRVQSPAVRLVVERERERIAFVAADYWDLTAAFPTDPAFTARLVALDGKRVATGR
                     DFGQDGRITRDDVAVVDEPTATSLRTRLDEQPYTVRSIDEKPWRSRPKPPFMTSTLQQ
                     EGGRKLRMSANQVMRIAQGLYEKGYITYMRTDSTTLSQTALDAARSQVRELYGADYVP
                     DQPRQYAGKSKNAQEAHEAIRPAGETFRTPDQVKGELRKDELGLYDLIWKRTVASQME
                     DAVGRTISMRIVAEARADGGQPANECTFGASGRVITFPGYLKAYVEDSDDDDAERDDA
                     ESVLPAVAEGDALPTPALTADGHTTSPPARFTEASLVKRMEELGIGRPSTYASIMTTI
                     QDRGYVFKKGTALVPTWTAFAVVNVLERQFGEYVDYEFTAKMEDDLDLIAVGEEDRVP
                     YLKRFWFGDDGASGGPGARKGLSVVVDEALEKVDAAEVNTFPLGMDENGEMVVAKPGR
                     YGPYVKRGEDDTASIPEGLPPDELTLDKALELLAAPKGDEPIGEHPESGLPVYVKTGR
                     YGPYVQLGDQDTLPPDEKPKMESLFKEMKPEEVTLDDALRLLSLPRVVGTDEETGLPV
                     LARNGRYGPYISKGDEKGADARSLEAEEELFTVDMDKAKALFAEPKRRRGQAAPKPPL
                     RELGDDPVSEKPVVVKDGRFGPYVTDGETNASLRKGDLVEEITIERAAELLQIRRETN
                     PPKKKAGARKKATKKKAAKKKTAAKKSAAKKKATKKASE"
     gene            20270..20704
                     /locus_tag="VLR27_03805"
     CDS             20270..20704
                     /locus_tag="VLR27_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02597.1"
                     /translation="MVDLLRFVHRLADDPAARDRIRRDPQAVLDVEVDDAATLTGEDV
                     EAVAEVLRRRLPAAAAALVELPAPSRPVGSETPRDAALRLLIGLCDALDGPAVQPVTL
                     PHRELRPDPPPPRQTARGGGAPVPTEPPGGRWLHPVIPDDDP"
     gene            complement(20776..21684)
                     /gene="arcC"
                     /locus_tag="VLR27_03810"
     CDS             complement(20776..21684)
                     /gene="arcC"
                     /locus_tag="VLR27_03810"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010635806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HSP02598.1"
                     /translation="MRIVVALGGNALLRRGEPMTTAAQRLNVRLACEALAPVALEHEL
                     VVSHGNGPQVGLLALQGEAYRPDEASPLDVLGAATEGSIGYMIEQELGNLLPFERQLA
                     TILTMTEVDPDDPAFDEPTKPIGPVYTAAEADALVASKGFTVRPDGEHFRRVVASPEP
                     KRIFELRPISWLLDRGCVVICAGGGGIPTMYEPGKERHLVGVEAVIDKDLASSLLARE
                     LGADLLVLATDVDGVYEAWGTPDQRRIDRIAPAEIDVGDLPAGSMRPKVDAACRFATA
                     TGHPAVIGALDDLASIIEGRAGTRIG"
     gene            complement(21692..22792)
                     /gene="aguA"
                     /locus_tag="VLR27_03815"
     CDS             complement(21692..22792)
                     /gene="aguA"
                     /locus_tag="VLR27_03815"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF010070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HSP02599.1"
                     /translation="MTATLGTTPAADGFRMPGEFERHLGTWMVWPERPDNWRLGAKPA
                     QDAFVAVATAIAAAEPVTMAVSARQYANCRSSLPAHVRVVEMANDDSWMRDIGPTFVV
                     DDRGGLRGVDWVFNAWGGLVDGLYFPWDLDDAVAQKVCEIEGADRYRATIVLEGGSIH
                     SDGEGTLFVTEECLLSRGRNPGLSKAEIERELVAYTRAEKVLWLDRGVHLDETNGHVD
                     NLVNVVRPGLVLLTWTDDASDPQHEISADARRRLESWTDARGRSIEVLTLPSPGPVHI
                     TEEESAGVDAVDGTLPRLPGDRLAASYANAYLCNGRVVVPLLDERVDDEALDVYGKAF
                     PDRDIVGVPAREVLLGGGNIHCITQQVPDPRA"
     gene            complement(22789..24192)
                     /locus_tag="VLR27_03820"
     CDS             complement(22789..24192)
                     /locus_tag="VLR27_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HSP02600.1"
                     /translation="MTTDRASGRRRVMGLRDITLFTVSAMLVVDQLTASASIGPSVVG
                     WWIVAFVVFLIPSAFITAELATTYPDQGGLYIWIKQAWGAKWAARTTYWYWVNVALWM
                     PSVFLLFAGTFAELFAEDLGKWPRAAIALGLTWLVVWIGVQKLEIGKWVNNLGAVLKI
                     TIIGALAVGGFYLLVRDGAANDLAAKDMFVPDWDAAKFFLPVLVYQLLGFELISSMAG
                     EVKDPERNMPRAFGWSAVIIAALYFFGTMGILMALSMQDLGLVSGLVDTFEAIFGTST
                     AGDVAVYGLGIAVLYTFVTNMTTWSMGANRAAAEAATDGELPAAFGREHPVRRTPTFA
                     FVAMGVISSVVLLGTTAILEEQDTLYFAIFAASSVVFLLPYVLMFPSFLRLRKIDPDT
                     PRPYRAPGGKVGGIAWTVLTTFCIVASLVLFLWTPGVVVDWAYTGWLVAIVVATLAAG
                     EVLVGRALRARRAGTET"
     gene            complement(24292..25320)
                     /gene="ptcA"
                     /locus_tag="VLR27_03825"
     CDS             complement(24292..25320)
                     /gene="ptcA"
                     /locus_tag="VLR27_03825"
                     /EC_number="2.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR04384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050231 - putrescine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009445 - putrescine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine carbamoyltransferase"
                     /protein_id="HSP02601.1"
                     /translation="MGRHFLDTNDFSRAELEEVLELTRLLKDADRDGCVPELLRRRSL
                     AMIFEEPSTRTRVSFEVAMVKLGGHALYLKPGEIHLGARESLADTARVLARMCDVVMA
                     RTLVHETVTELARWSDVPVINGLTDYNHPTQAICDVFTMSEHLPPGKALSDCNVVFVG
                     DATNVCSSTMHITTKLGMHFVHAGPAAYQSPEAWVKLAEQNVADAGSGSVRVTDEVED
                     AVREADFVYTDLWWWVDQEDEIPQRRAAFMPRYQVNEALMARAPADARFMHCLPASRG
                     VEVTDEVADSPQSVIFDQAENRLHAEKAILVWTTAPDIDFDISPELQALHAGRIREQL
                     GRIGRRAD"
     gene            complement(25516..26700)
                     /locus_tag="VLR27_03830"
     CDS             complement(25516..26700)
                     /locus_tag="VLR27_03830"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HSP02602.1"
                     /translation="MSGSVILTGARTPIGKLSGAFASMAATDLGGVAIKGALEKAGIA
                     PDQVDYVVMGQVIQAGAGQITARQAAFNAGIPMDVPATTINKVCLSGLNAIYLADLMI
                     QAGQADIVVAGGMESMTNAPYLLPGARGGYRIGDQKVVDSMMHDGLFCAFDMCAMGAG
                     TEKYAASAGLERQPQDEFSAQSHQRAAAAAKNGLFDDEIVPVPVPQRKGDPVMVSEDE
                     GVRGDTTGESLAQLRPAFDKAGNITAGNASQISDGASATIVVSRAKADELGITPLGEV
                     VGYGQVAGPDPSLLTQPSRAIKDALQRSGMSQDQLGLYELNEAFAAVGLASMEDLGIS
                     DDIVNVNGGAIALGHPVGMSGNRLALQLLLEMKRRDVEYGAAALCGGGGQGDAIIVKT
                     IK"
     gene            complement(26774..27940)
                     /locus_tag="VLR27_03835"
     CDS             complement(26774..27940)
                     /locus_tag="VLR27_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02603.1"
                     /translation="MTTMTKAYAITAEAVAAETEANGGDIAAIDVSKVVQLDDLELRD
                     LGPHDVKLKILAVSAEHNIDHAATADTINIAEARGGKMYPGNSAVGEVIEVGADVSAF
                     APGDVVITHCNGDPDRFGFPLRIWAYDMPDSIGWYSKEAVVEDWQIIKAPLDSGLNLW
                     EIAALPLRAPTAYHLWRRAIGIYRVKVPYERQARLNVLSFGGGVGELFLMLAKAEGHN
                     AFFCAGSKERRDALEKQGIVGIDQKQFNRFASSDDVKAFNKHVKGLTDGVGMHLVCDM
                     LRGPVFEAGLAVQARLGVNVSAGWQLSQVVSYNSTVQSVKQVTIDHTHYETPEGCEAA
                     TELYGSVFKPTIHDEIYSFEDLPRCFQEMHENTQTGIPIIRVADEMPDSVKDLV"
     gene            28045..>28424
                     /gene="mce"
                     /locus_tag="VLR27_03840"
     CDS             28045..>28424
                     /gene="mce"
                     /locus_tag="VLR27_03840"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HSP02604.1"
                     /translation="MLLTEIDHVAIAVKDLDAAIAYYGKAFGAEVHHREVVDSDGVEE
                     ALVKVADSYIQLTAATRDDSPIAKFLEKKGEGLHHIGYRVDDCAEALESMVAAGATPI
                     DKAPRPGSRGTTVAFIHPKGSLGTL"
BASE COUNT         4018 a         9813 c        10530 g         4063 t
ORIGIN      
        1 tgcgctcggg accggcgagc acgcggtcga tggcggactc gacggtgtgc atgtcgatgg
       61 tgtcgaccga gcgccgggcg gccatcagcg ccgcctcgtt catgaggttg gccaggtcgg
      121 cgccggtgaa gcccggggtg cgacgggcga tgaccgcggc gtcgacgtcg tcggcgaggg
      181 gcttgccctt ggcgtgcacg tcgaggatgg ccttgcggcc ctcgaggtcg gggcggtcga
      241 ccacgatctg acgatcgaag cggccggggc gcagcagcgc cgggtcgagg atgtcggggc
      301 ggttggtggc ggcgatgagg atgacgccgg tgttggcgtc gaagccgtcc atctccacca
      361 ggagctggtt cagcgtctgc tcgcgctcgt cgtggccgcc gcccatgccg gcgccgcggt
      421 ggcggccgac ggcgtcgatc tcgtcgacga acacgatggc cggcgcctgg gccttggcct
      481 gctcgaacag gtcgcgcacg cgggaggcgc cgacgccgac gaacatctcg acgaagtcgg
      541 agcccgagat cgagaagaac ggggcaccgg cctcgccggc cacggcccgg gccagcagcg
      601 tcttgccggt gccgggcggg ccgaccagca gcacgccctt ggggatcttg gcgccgatgg
      661 cctggaactt ggccgggctc tggaggaact ccttgatctc gtggagctcc tcgacggcct
      721 cgtcgcagcc ggccacgtcg tcgaaggtga ccttgggctg gtccttgttc cactgcttgg
      781 ccttggcctt gccgaactgc atgacccggc tgcccccgcc ttgcatggcg ttgaggaaga
      841 acaggaacag gccgaacagg aggagcagcg gcaggatgct gaacagcagg gagacccaga
      901 tggactcctg ctgctgatcg gtctcgatct cgatggcggg ctcggcctcg gagagctcga
      961 tggtgagctc gtcggcgaac tccgccgggt acgacacctt gtactcggtg ccatcgcgca
     1021 gctcgccctg gacgtcgtgg ctgcggtcct tgatggtcgc cgtctcgacc tcgccgtcgg
     1081 cgacgtagtc gcggaactcg ttcagcgtga actgctcggg atcggacccg gagcgcagga
     1141 acgaggacgc cacgagggcc gccaccaggg cgagtgcgac gtagatgagc gttgggcggc
     1201 gcagcttctt cttcacagtc cgcaccacgc taccgaccga cccaggatcg ttgacatcgg
     1261 acgggctact cgtcgccccg gtagtcggcg atgtggggga ggttgcggta tcgctccgcg
     1321 acgtcgaggc cgtagccgat gacgaacgcc ggcgggatgc ggaagccgat gtagcgcagg
     1381 tcgagctcgg cttgctggtt gccttcgcgc acgagcagcg cgcagacctc gagcgacgca
     1441 gtgccacggg actcgaacgt cttgcgcagg taccgcagcg tgaggccgga gtcgacgatg
     1501 tcctcgacga tgatcacgtg ccggtcgtgg aggtcgatgt cgaggtcctt cacgatgcgc
     1561 acgacacccg aggagcgggt gctcgccccg taggacgaga ccgccatgaa gtcgacctcg
     1621 agggggatgt ccaccgcacg cgccaggtcg gccatgaaga tgaaggcccc cttcaggacc
     1681 gcgacgagga tgggatcctt gccggcgtag tcccgggcga tctcggcgcc gagctcggtg
     1741 atccgttcct ggatctgggc ctccgacagg agcacgtcgc cgatgaggtc gtcaccctgg
     1801 gcagccatgc gggcggaacc tagcggtcgc cccgacccgc cgacccatcc gccgtgaggc
     1861 gctgcgcccg ccgccggacc cggacgccac cggccacctc ggtgccgacg ccctcacccc
     1921 gggccaccgc cagcacgcgc tggaccgcag ccgcatccgg ggggtgcccg ccgggcagca
     1981 gggcccggag ccagcgccgc acggcccgac ctgccagggg tggcggtgcg gccgcgaggg
     2041 cgggcgcatc ggtggcgtcg agggcggacg ccagctggtc gaggaggtcg acgtcggcgc
     2101 gcgcgagctc ggcgaagcgg cacagcagtg gcacgacgtc gcgctcggcg acgtcgtcga
     2161 gcagcggcag cacctcggca cgcacgcggt tgcgacggaa ccgcgggtcg tggttcgtcg
     2221 ggtcggacac cggctcgagt ccgagctcgg cgcagagtgc gtgggtctcg gatcggcgca
     2281 gcgccaggag cgggtggtgc tcggcccgca tctcggcgac cccgtcgagc cccgatcccc
     2341 ggagcagcgc gagcaggacc gtctcggcct ggtcgtctgc ggtgtgcccg tagagggcgt
     2401 ccggcccgag cagccggcgc cgggcggagc gcgcccgggc ctcgagatcc ccgccggggt
     2461 ggacctcggc ccgctcggcg cggaaggcgg cgccgaagcg actcgccgca ccggccacca
     2521 cgtcggcctc ggcctcgctg ccggcgcgca gcccgtggtc gacgtgcacg gcggtgacgt
     2581 cgagtccggc atcgcaggcc aggaccagca gggcgaggga gtccggcccg ccagagaccg
     2641 cgcacgtcac cgggccgcgg gacgggaacc ggcagcgccc ccggacggcg tcgagggtgg
     2701 cggacatcgc cctcagccgt cgacgacgac ctgaccggcg cgcaggcgcc ggagcagctc
     2761 gggcaccgat tcgtggccgg tcttgcggat gatccggcgg aggctggcgt agatgttgga
     2821 gcggctgacg ccgaggtcgc cggccgccgc gctcacggac ggcgccgcgg cgagggtgcg
     2881 caggaggctg cgctgcttct cggtgaggtc accgccgacg tggaccgcgc cgtcgtcgac
     2941 gtggtgctcg tcgcgagggc gggcgtgctc cagccgggac agccgtggtc cggcgcgctc
     3001 gtcccggggg gcgtcggcgt cgccgccccg ctggatgcgg gcgagctcgg acagggcccc
     3061 cgtctgggcc ttgcgccgct ggtcgcgctc ggagcccccg gccatgacat cggcgatggc
     3121 cgcccggagc tggtcggccg acaccggctt caccaggtac cgcaccgcac cctcgatgcc
     3181 gccccggagg cggtcgtcgt cggtgcccag cgccgtcatc atcagcacct tcaccgagga
     3241 gatctgctcg tccaggtgcg ccttcagctg ctcgagcacg ctccagccgt cgaccccggg
     3301 catcatcacg tcgagcagca ccacgtcggg cacctcgacc tgcaccgcgg cgagggcctc
     3361 ggcgccgtcg gctgcggtca tcacgcggtg cccgtcgagc tcgagctgga gctgggtcag
     3421 gtagcggatg tcgggttcgt cgtcgacgac cagcacggac gccacagctg cctcctcccc
     3481 acgagatcgg acgctcatca tgccaactgc tcgggcgcgc ctggtggcgg agccccgggg
     3541 acggtcaggt gacgagctcc tcggagagcc gcacgcgctc gacccactcg tggggttcgc
     3601 ggatctccgt cagcgtgggg agccaggacg gaccccgcca gaccggcgcc agcgcgccct
     3661 tgcccccacc gaggcgctcg acctcggcga tgaagtcctc gccctgctgg tactgggcga
     3721 gcttggcctc gaggccgatg aggcgctgga cgagcttggt gagtccggtg gccgacgccc
     3781 ggcggttgcg cagcacgcgc ccgaaccgct cggccgaggg cacgtgcccc tcgcccgccc
     3841 ggtccatcgt cacgtcgccg tggccctcga gcaggctcat cagcccgccg atctcgtcga
     3901 gggcggcgcg ctgctcgggc ccggccagca ggtgggcgag gccgccttcg ggcagcgggc
     3961 tccggccggc ccgcagctcc tcggcggtgt gcttgagcgc ccgcagcagg accatggggt
     4021 cgaggccggc cgcggacagg gtgcggtcga tcaggccgag gtagtggtcg cgcatccagg
     4081 gcacgccggt gaactgggcc cggtgggtga cctcgtgcag ggccagccac agccggaact
     4141 cacgcggcgg gaagccgaac ttcttctcca gggccaacac gttgggaccc acgtagtaga
     4201 cgaggtcggc gtcggcgggc tgaccgtcgg tgtcgtcgtc ggacccgccg gcgaccagca
     4261 ggtcgtactg gcccagcacc cgacccgaca tccagccgag caggaggccg acctcggccg
     4321 cggcggcggc gcgcatgatc ggcacggtgg gcccggagac ccgcggcgcc atcttctcgg
     4381 tgaaggggcc gaggagccgc tggaacgagg agatgttggc ccggacccag ccggcgcggt
     4441 cggtgacctt gccccgggcg gggccggcga gggagtgcag gccggtctcg gcggcgacca
     4501 gctcctcggc gagggcggtc agctcggcga agtccggctc cagggactcc cagtggtacg
     4561 agtccgcgag cggctcacgc cctgcggccc ggacggcgat gcgttcggcg aggtcccagt
     4621 cgaccagctc agccacgcgg ctggtcgacc cccgggtact gcggcttgac gaccttgcgc
     4681 aggtagagca ccaccgtcat gacgacggcg ggcacgacga gcgtgatcat gaggacgacg
     4741 gcgatccacc agtgccagat gatggcgagc atggtccgac tctacgccga cgcccgggtc
     4801 ggtccgacat cgccggctca gccggcgggt ggctcgtgcc cgatcgcgtc ggcgatgcgg
     4861 gagcggaggg actcgggaac ccggtcgcgg cacttctggg cgatgtactg cttgatctcg
     4921 gcctcgaagt cgaaggcctc gaagcagggc gggcactcct cgaggtgctc ctggatcgcg
     4981 acccgcttcg cgtcggtgag ctcaccgtcg aggaagtggt agagctcgtg gagcgtctcg
     5041 ctgcagtcgc cgtggtccat gggatctcgc aaggtcgtgt cagccggtgt tggccggctg
     5101 gctctcgtcg ggtgcgtcgt ccgtgcgctc ggtgaggccg cgagcgacgg cgaactcgta
     5161 caacgacttc tggagggctt ttcttccccg gtgcaggcgc gacatcacgg tgccgatcgg
     5221 gatgtcgagg atctcggcga tctccttgta ggcgaagccc tcgacgtcgg cgaggagcac
     5281 cgccatgcgg aaggtctccg gcagggattc cagcgcgtcc ttcacctcgg cctcgctgaa
     5341 cagctcgagg agctcgtcct cggccgaccg gccggcggtg gcgccctcca ggccccccag
     5401 gcgccggtac aggtacaggt cctcgacctc ggccaggtcg gtctcgtccg gtctgcgctt
     5461 cttggaccgg tagatgttga tgtaggtgtt cgtgaggatc cggtacagcc acgccttgag
     5521 gttggtgccc tcctggaagc cgccgaagcc ccggtaggcc ttgaggaagg tctcctggac
     5581 caggtcctcg gcgtcgctct ggttgcgcgt catgcgcagc gcggcgccgt agagcgagtc
     5641 catgaagggc atcgcctgcg ctgcgaacgt cgcctggtcg gccatcgggg ccgaccctat
     5701 ccctgcgccg gctcgggtcc ggtggtcggt cacgccggga cggcgtaggc gaccacgttg
     5761 ctctcgtagc tgcgcagctc ggggttgaac tcgcccccgc aggtgatgag caccaggcgc
     5821 tcggggacgt cccgggcgaa caggtcgtcc gggagctcgt ccttgtcgaa cgtgaccacc
     5881 tcggtcacga ggtaggcctc gcgggacccg tcgtcgaagg ccacggcgac ctcgtcgccg
     5941 gcccccaggc ggtcgaggcg ccggaacacg ccgtcgacgc cgtcgtaggc gatgtgggcg
     6001 gcgagcacgg ccgagccggg ctcgcccggc gaggccccga accggtacca accgacctcg
     6061 tcggccggcg ggatctccat ctccccgttc ggctccaccc ccaccggctc gacgggggca
     6121 ccctcgacgc cgatggccgg gagggacagg ccggtcaccg tgcgggtcgc cgcctggtgc
     6181 tcgtccgggt cgtagcgggc cgagtcgagg ggctgcaccc gctcggccag agccgacgtc
     6241 ggcctcggct cggggaccga ggtctcgggc gtggtggtcg tcggcgccgt gggcgaggcg
     6301 acgaccccgt cctccgacgc gccgtcgccg gccggggcgc cacaggcgac cccggccagc
     6361 aacagcagca gcagcgccgc cgccgccgtc acgctgcggc ggcgctccac gtcagcggac
     6421 ccgggcggcg acgagccggc gggccgggac ggcggtcgcc agcagggcga caccggcgag
     6481 cgccagcagg ccgaccggga ggccggcgtc gtcgctcacc gggctgttgc cggtgttgac
     6541 ggcggtcggg gcggtgccga ggccctcgat gctctcggtg agcacggtca ggctgtcggc
     6601 gtcgagcgag ccgaccgcgt agacgatgag gctggtcccg tcggtcacgg cgaggtcggc
     6661 ggggccgatc acgaccgggt cggcggcgcc ggccggcacg accgaggcac tgatggtgcc
     6721 ggcggggagg tcggccgagg cctcgttcgg gttggacagg ttcgtgaagg ccgcctcacc
     6781 gttggccagg acgtcgaccg caggagcggc agccgtgtgg cggaccgtca ggcgaccctc
     6841 gccggcggcg gtggcgctgg tgtcgttctc gaacacgccc agcgtcgggg tgccctcggc
     6901 atcgaggtgg gcgatgatcg tgtagttgcc gctcgccggc agtgccgtgt caccggcgtc
     6961 gatggcgacg gtgtcggtgc cggcggcgtt caccgtgagc ccggtgaggg tctggccggc
     7021 gaggccggac aggtcctggg tgtccccgaa ctggaagtcg gagaacacga cctcgccgcc
     7081 ggcgaccacg tccacgggcg tgtcggggat cccgtggatc aagtggatcc gggcgctctc
     7141 ctgcgcggcg ctgggcgtgg cgagcacgac cagcaccgca gcagcgagca cgccgatgat
     7201 ggtggggatc tttcgcatgg aggttcctcc tggagcaggg ttaagtgtct ttcatccctg
     7261 gttacggaac ccccggatgg tgcggatggc cgatcagcgg aatccggtga atgccccttg
     7321 tgccgggttc gcgaactccg acacgaccac gccaccgtcg aacagcgacc acagcttccc
     7381 ctggacgacc agcgcccggg acgggtagcg cccgtccgcc gtgaccagcg cccgctcggt
     7441 gatgccgctg tcggccacgt cgaggacgac gatcccgctc tggacgctcc cggagccgaa
     7501 ccgctcgagg ggcacgatca ccgcggattc gggggcccag tgcaggaacg ccttcgggtc
     7561 gtcgccggcg atggaccagg cgtcgcgctc ggtccaggtc tgcagctggg tcggggcggt
     7621 cgggtcggcg acgtcgaaga gcgagaccag caggccctgg tcccggccct cgaggtcggc
     7681 gtcccgcccg atgccgacga ggcggccctc gccgaccggg tgcaggtagg aggagaagcc
     7741 ggggatcttc agctcgccga cggcgcgcgg cgccctcggg tcgctgaggt cgatcacgta
     7801 aagcgggtcg gtctggcgga acgtcacgac gtagccgacc ggccccatga agcggaccga
     7861 ctggatcgtc tcggtcaccc cgagcccggt gacggcgccg acctcgacca gcgcaccgcc
     7921 atcgagctgc agcacccgga caccggactg gctgtcctcg ccgccgcccc agggcgcgcc
     7981 ctcggtcgtg gccgcccgca ggtacccgtc gtgctcggac agcgagtact ggttgaggag
     8041 gtgcccgggg atctcgccgg cgccgaggtg ggcagctggg ccgtcgccac gcaggtcgaa
     8101 ggcgtggagg gcggtggaga agtcctcccc cacgatccgg ccctgctcct gcagggggac
     8161 gtaggtgttg gccgccacgt agagggtgct ggtcgaggcg tagaccgtcc cggcctcggc
     8221 gacgaccgtg gtcgtgtcag ccgggtcgaa gccgtcggtg aagtcgaccc gcaggaccga
     8281 ggtctgggcg atgcccgcgt tcacctcggg gacgaagacg tcctcgcagg cgaccaggct
     8341 gccctcggtc gacgtgcggg acgccacctc gccggcgtcg gcgaccgtcg tgcggaacgc
     8401 gggcagccag tcgtcgatcg tcgtgtcggc gatgccggcc tccatggccg cccgggccga
     8461 gtcctggtcg ctcccgtcgc cgaggcgcat cgatgcgtcc tcgaggagcg gctgggcgtc
     8521 ggggtagctg ctcaccacga cgcgggcgac gccgtcgacc acccgggtgg acacgtggtg
     8581 cccctcgatc tcggtggtct gcacgatctg gggggaccgg cggtcgctga cgtcgagcag
     8641 ctgcaggacg gtgccggccg cgaagctcgg cggctccgtc ggcatcggga acgggtccac
     8701 cgggatcggc tccgggagcg gctcgggctc gggcagcggt tcgatcggct ccaccggctc
     8761 gggcgcgagg gtcgtctccg tcggctcgac cggaacgctc ggttcggccg gctcagacgg
     8821 ctcggtcggc tcctcgggat cggtcggctc gggcgggtcg acctggccgg gcggcacggt
     8881 cgtctccgtc ggctcgaccg gctcggacgc gtcgagcccc tcgtccggtg ccggaccgga
     8941 cacggcggcg ccgtcctcga ccggcatcgg catgggcatc gggtacccgt agccgccgct
     9001 cgtcacgacc agcatccggt cgccctgcag gagcagctgc gccccgtagg aggcgagcgg
     9061 cgtgcggctg agcacctcgg ccgtggcgcc gtcgaggatc accaggtggg cctggtccgg
     9121 cccgtggtcc acgacgtaga cgaactcgcc gtcgttctcg accacgtccg gctcgtcgat
     9181 gccggcctcc tgggtgttgg tgtccgacga cgacccgtcg gccgcctcga gctgggcgct
     9241 cgcctcgtcg ggtgccgcgg cgcgctccgc accggcgtcg gcggacatgt cgtcaccgac
     9301 gtcctcgaac tccgccgatg tggtcgccgc gccgccctcg gcgaagccga acatctggtc
     9361 cgacagcacc gcgggcgcga acgactggaa gtcgtcgagg acgtcgccgc agctccggtc
     9421 gctctgcagg gccaccggag cggcgccccg gaggccctgg aacgcctggc cgccactgga
     9481 cggcggcgag ccggagtcgc tgctgcaggc ggtggccgcg accgtgacgg cggccagcac
     9541 ggcgaaggtc cggatccccc ggtgggcgtg tcgagtgcgc atggccatcc gacgtccggg
     9601 cctgccgcct ggttcccggc tcgggctcag cgctccaggc gccggacgcg gacccgggtg
     9661 atcttgtgcc ccaagacgtc ctcgacgacc agctcgtggc cctgcacgtc gacggccgtg
     9721 cccacgtccg gcacggtgcc gtgcgccgcc agcacgaggc cggcgagggt ggcgacctcg
     9781 tcgtgggtga acgtcagccc gtggtcgtcc cgcagctcgg ccagggtgac ctcgccgtcg
     9841 aggctgatcg agccatcgtc gtggtgcacg gcggcagacg accccgactc gtcgccctcc
     9901 tccatcacct cggcgatcac gtcgtcgagc gtcacgacgc cgagggttcc gccgacctcg
     9961 tcgaccacga ggcaggcgtg ggtgcgggtg cgcgtgaacc gctccagcag ctgctcggcg
    10021 ctggccgtgg ccgccacggt cgggaggggc cggatgaggt cggcgaggac gacggtcgat
    10081 cggcgcgacg cccggatgaa gtccttgatg tggagcacac ccaccacgtg gtcgaggtcg
    10141 ccgtcgatga cggggtagcg gctgcgcggc gacgccgtga cctgcgccgc cacctcatcg
    10201 gcgctcgctg ccaggtcgag cacctcgagc cgcccgcggg aggtcatgag ctcctcggcg
    10261 cggcgctcgt cgagctcgaa gatgttgcgg atgaggtccc gctgcagcgc gccgagctgg
    10321 ccgctgctcg ccgcctcgtc ggtgacgatc tcgagctcgg cgctggtgta gagcgcggtg
    10381 ctcttgtccg gctcggggat gcgcagcagc cgcatcagac ccagcgccgc tgcgttgagg
    10441 acggccacca tcggccggaa gaccaggccg aaggcccgca tggccgggtt caccctcagg
    10501 accacggact cgggcgcctg gagcgccagg gccttgggga tcatctcgcc gaagacgacg
    10561 tgcaggaacg tgatggcgct gagggcgatg gcgaagccga cggcgtgcga tgccccgtag
    10621 gacaggccca ggccgtcctc cagcggcttg tagagccacc ccgccacggc cggctcgccg
    10681 tacatgccga gcccgatgct ggccagggtg atgcccagct gggcgatggc gatgtagccg
    10741 tccttgccgg tggggcggtc gaagacaccc agcagccagc gagccgcggt gctgcccgag
    10801 tcggccatgg cctggacgcg gctgcggcgc gcgccgacca gcgcgaactc ggcggcgacg
    10861 aacagcccgt tgaccagcac cagcacgagc acggcgatga tggggagcac gacggtggtg
    10921 atcacgccgc accgcccggg acgtcgaagc tggcccgggc cacggcggtg ccgtcgaccc
    10981 ggtcgacccg gaggaccacg ccgtcgggcc cgacgggcac ctcgtcgcct tcacggggac
    11041 ggcggccgag ctcgtgccac accagcccac cgatggtgtc gacgtcgtcc tcggtcaggg
    11101 tcgtgccgaa gcggtcctcg agcacctcga ccaggacgtc gccccggacc gacacccgcc
    11161 gctggtcgac cgtgatcggg tcctcctcga cgtcgaactc gtcgaggacc tcgccgaaga
    11221 tctcctcgat ggcgtcctcc atggtgacga gaccggcgac ggagccgaac tcgttgacca
    11281 cgagggcgag gtgctcctcc tcgtcgcgca gctcgtccca caggcccggc accgtgagca
    11341 cctcggccac ggccggcacc ggccgggcca ggtcgccgac cgatcggctg ccgtcctggc
    11401 gggccgcgac ccagaggtcg cgcaggtgca cgatgccgac gatgtcgtcg tcggagccgg
    11461 tgaccgggaa ccgcgtgtgg gcgctgtcgg cgagccgggc gagggcgtcg tcgaccccca
    11521 gcgtggcggg caccgtggtg agctggcggc gcggggtcat gatctcgcgc accaggcggt
    11581 cctcgacgtt caggacgccg gcgagcatgt cccgctccga cgcgtcgatg aggccgccct
    11641 cggcgctggc ccggtagagg ccggccagct cctcgggtga gtgcacgtgg gcgtgcgagt
    11701 ggtcgacggc gaggccccag agcttcatca ggcggaaggc ggacccgttg aagatcgcca
    11761 ccagcggccg gaagagccac tggctgatcg ccatcggggg cagcgtgagc atcgccagct
    11821 tctccgggta gcgcagcgcg gcggtcttcg gcagcagctc acccagcacc acctggaccg
    11881 acgtcacccc cacgagcacg atgagcacgg ccaccgcccg cccgccgacc gagccgaggg
    11941 cgggctccag caggggcgtg agcgcagcct ggccgtacgc gccggccacg aggctcgaca
    12001 gcgtgatgcc gatctggcag gcggccacgt agttgtcgag gcgcttcggg tcctcgagga
    12061 tctcgagcag ccggccggcg gcggcgctgc cgccggcggc gtcctcctgc acgcgcgagc
    12121 gccgggaccc cacggtggcg aactcggccg cgacgtagag cgcgttgaac gacaccatca
    12181 ccgcgatgag cagcaggggg atgacgatgc tcaacgggtc tcctcggagg ggtgggcggt
    12241 cacgtcgtcg ggaccccgcg ggatggcccg gacctgggtg atggcgtggc ggtgcaccgt
    12301 atcgacctcc agcacccagt catcgagggg cacccggtcc cccggcgtct ccggcacccg
    12361 cccgaggcga tcgaggatga ggccggcgac cgtcgtgtac ggaccgtcct ccacaccgtc
    12421 gatgccgacg tcggtcaggt cgtgcaccgg gaactgcccg gggagcagca tcgagccgtc
    12481 gtcgccgtgc tgcaccgaca ggatgtcgcg gtcggtctcg tcgtagatct ccccgaccag
    12541 ctcctcgacc aggtcctcca ccgtcacgat gccggcaccg gatccgtgct cgtccacgac
    12601 cacggcgagc tgggcgtggg actgctgcat ctggtggaga gcatcgagca cgccggccgt
    12661 ctcggggaag gcccggatct cggacacgac ctcgccgacc gggcgggatc cgagcccgag
    12721 caggtcgcgg aggtgcacca cgccgatgac ctcgtcgagg ttgccgttga cgccgaccgg
    12781 tgcgcgggag tggccgctgg cggcgagccc ctcgagggcg tcggccccgg gctgcgccgc
    12841 gtcgagcacg aagacctcac ctcgcggtcg gaggacctcc tcgagcgtcc gctcggagat
    12901 ctcgaacgcg ccgtcgatga tcaagcgctg cttgggcgtg aagctcgtct gcacggcgac
    12961 catgtcgcgc agctcctcct cggtgatctc ctcgcggtcc aggtgcgggt caccccccat
    13021 gagccgcacg gcgatgtcgg tcgagtgcga gagaagccac acgacgggtc gggccagcac
    13081 cgagatgaac gccagcgggc gggcggtgac catcccccac cgctcggcgc gctgcatggc
    13141 gacccgcttg ggggccagct cgccgaagac cagggtcacg taggcgagca ggagggtgac
    13201 caccacgacg gccacgggct ccgaggcccg accgaggaac ccgagcggct cctccagtgg
    13261 ctcggcgagc gagacggcgg cggccgccga cgccaggaac ccggccaggg tgatgccgat
    13321 ctggatggtg gcgaggaacc ggttggggtc gcgagccagg tcggcgagca ccgcgccggt
    13381 gctggactgg cgctcgaggc gctggagctg gccctcgcgc agcgacacca gggccagctc
    13441 ggtgccggcg aaggcggcgt tgaggaggac gaggacggca acgagtccga gctcgggcag
    13501 gacatcacgc acaggacaac tctcagggtt cggcgccaca cggggcggac gggacggacg
    13561 cgcggggccg acgtgggctc agcgacagga cgggcgctca ggcgcccggc ggtccgcgca
    13621 gcgtgggtgg gctgcagccc cgatcgatcc gagggtcctc cgtgatctcc gagcaccggt
    13681 gcgggcgcgc gtcgagccgc ccttccacac cggacgacgg tccggagtcg gcgacctcgt
    13741 gggcgccctg ctcgtactgg accgggtcct ggcgggccgc gtcgtcgacg atcggcgcgg
    13801 cgccgctggc atcggtgacg gtggtgtcgg cgccggccac ggcgaggagc acgccgacca
    13861 gggccagcac cgcgaggacg agcgagcgcg cccggatcgt cgccggttgc ggaggggggt
    13921 cgtcgctctg catgtgctga gagcccgaag tgggaattga acccacgacc tacgctttac
    13981 gagagcgtcg ctctgccgac tgagctattc gggcgggacc ggagagcgta gcgcggaggt
    14041 cccggcggtc cagatcctca aagaggatcg aggccgccga ccatcgcctg cagcaacaag
    14101 gtctcgttgg ggttgcgcac gagcgcctcg gctgcctcct ggacggcccg cagggcggcg
    14161 aggtgcgggg tcgggtcgcc ggccgtcgcc agccggtcgc ggtactcgcc ggcgagcacg
    14221 gcgagcccga agcggatctc ggcgttgcgg tggcggcgca cctcccgctt gtgcttcttc
    14281 tccaggtcct tcagcggcgt ggtccgcatg ccgtaggcct cgatctgctc ctcgagcgcc
    14341 agccgctcgg cctcgtggcg ctcgtcgagg gcaccctgcg cggcatcgat gtgggccacg
    14401 acctcgtcga cgagggcggc ggccgtgtgc ccggtgcggt cgacccggtg cgggatcgag
    14461 gcccacgagt cgcgccgcac gacgagcgac gggtcgcggg ccagcaggcg ggcccggtgc
    14521 aggtcgccgc cggccgccgc cgcggtggtg gctgcgacct cgcgatcgat gccctcggcg
    14581 acgaggcggt cggtgacggc atcctggggg acgggtccga ggtcgatgcg caccgagcgg
    14641 ctggcgatgg ccaccagctc gttggggacc tcctcggcga ggatcacgaa caccgtggac
    14701 gccggcggct cctcgatcgt cttgagcagg gcggggccgg cctgctggac cttgtggaac
    14761 tcctcgagca ccagcacctt gcgacggccc tcgaccgggc tccggctggc ggcggtgacg
    14821 atgtcctcgg cgtcacccat cgtgagcgct gcgccctcgg gttcgaacac ctgcaggtcg
    14881 gggtgggact cgtccaggca cagctcgatg gcccgctcga cgtcggcgcc acgccccgcg
    14941 gcgtcgatgg cgagcagggc ggcggcgaag gcgcgagcag ccgctcgctt gccgctgccg
    15001 gccggcccga cgaggaggta ggcgtgcacc ggacgggtgg cggcggcctg cagcgcggcg
    15061 acggcccgcc cctggccgac gacgtcgtgc cagaggtcgg cccgctcgac gagggcctcg
    15121 acgtcggccg gggcgagggc ggccccgagg tcggcggtgt cggtcacgcc cccatcctgc
    15181 ccgacgacct cgacctgccc gctctcgacg cgcagaacga ccacggacgg tcgatctgcg
    15241 cgcgaggaac aacgggcacc tggttcctgg cgcgcggatc gaccggatcc ggtcgatctg
    15301 cgcgcgagga acgtggtggt caggggtcga ggtcggggag gcgggcggtg accgcctccc
    15361 acacggcggc ggcgatgtcg tcggggtcgc cggtggcgtc gatgaccacc caccggtccg
    15421 ggtgggatgc ggcctgggcg aggaagccgt cgcgcacccg ctcgtggaac ccgtcggcct
    15481 cggactcgag ccggtcgggg gcctggtgcg cgatgcgctg gcgggccacg tccaccggga
    15541 cgtcgaggag gacgaccacg tcgggcagga gctcgtcggt ggcccaccgg ttgagccgct
    15601 cgacgccggc ggggtccagc ccgcgaccgt ggccctggta ggcgatcgag gagtggaggt
    15661 agcggtcggt cacgacgtgg cggccggcgg cgagggcggg gcggatgacg gtggcgacgt
    15721 gctgggcccg gtcggcggcc atcagcagcg cctcggtgcg ggggtcgagc tcgcccgtgg
    15781 ccgggtcgag gaagatgcct ctgatcgctg cgccgagcgg cgtgccaccc ggctcgcggg
    15841 tgagcagggc gtcgaggcgt cgggcgagcc gctcggtctg ggtggacttg cccgccgcct
    15901 cgccgccctc gaaggccaag aagcgcgccg tcatccgtcg acctggtcgt cgccggtcgg
    15961 cgtcgcgtcg gcgcgcgccg tcccgccgcg gtggacggcc gggggaccgg tgacgagctc
    16021 cttgcgggcg gcgtcgagca tggagtgggc ggcgatgaag ccggccacca cgatgatcgc
    16081 ggacgcggtc cacagcgtga gccggacgcc gggaaccgcg atggacacgc cgaagagccc
    16141 gacgtggccg tcgtcgaaga ggctctcgga cagctcaccg agcagctcgg acaggaacgg
    16201 cccgacggcg aaggcgatca gcacgcagag gcgcacgagg gtgtagagcg cggagaagat
    16261 gcggccccgc agctcgtcct ccacgtgctc gtgcagcatc gtgaagccga ggacgtacac
    16321 gcacccggcg cagatgccga gcacgaacac gaacgcggcc gccagctcca gcgccgacat
    16381 cgacgcggcc gtgccgagcg agaacccagc gacgaacacg gcgacgacga acacccactc
    16441 cttcggcacc cggcgctgga acaccgagat ggtgatgacc ccgccggcga cgccgagccc
    16501 gagggcgaag atgaggacgc cgaagccgga ctgccccgcg cccagcacct ggtcggcgaa
    16561 cacgggaccg agcggcacca gcatcccgcc gccgatcagc ccggttgcga ggccgaggtt
    16621 caccgcccgc acgaccgggt tgaggaagac gaactgccag ccttcgcgca gctcacgcca
    16681 ggccgcaccg aactcgatcc gcttcgcgcc acgggcgcgg tccgccttcc ggcggcgggg
    16741 caacggcagc gaccagatca tgagcgccga gagcaggaac gtcccggcgt cgaagtagaa
    16801 ggcgaggccc tcctgggtcc cccggacgac ggtgagcagc tcgaagtcgc cgatggtgtc
    16861 ggacagctcg gccagcaggg agaacagccc ggcggcgatc gggatggtgc cgtacgcagc
    16921 ggcgagcgag agcgagttgg cggtggtgag gtggtccgac ggcaccaggt ggggcaccga
    16981 cgcctccttg gcgggcgccc acaggagggt gaagacctcc atcacgaggg aggcgacgat
    17041 gaggccgggc acggtgtcga cgaacggcag cgacgccagc accagggcac ggccgacgtc
    17101 gcacatgacc atgaggcgct tgcggtcgaa gcggtcggcg aacaccccgg ccgccgggcc
    17161 gaggaagaag ccgggcacga tgcgggccga catcaccaac ccgaccgcag cctcggggcg
    17221 gtcgctgatg cgggtggcga gggcggcgat ggcgaggaag cccagccagt cgcccgtcgc
    17281 cgagacgacc tgggtcagcc agaggcggaa gaacgcggtc gatccgaaca ggcggacgtg
    17341 cccgcggggc tcgccctcgg cgagcgcctc catggcgagg gtgggcacct cggcctgggt
    17401 gtccggcgtg cccaccgggc cgtcgccggg gcgcggcgtc gtcggtcgcg cttcggaggg
    17461 ctccgccacg accccgacgt tagagaaccg ccgggctcga accgccgatg tccgcccgag
    17521 ccgctactcg gacgccttct tggtggcctt cttcttggcc gccgacttct tggctgcggt
    17581 cttcttcttg gccgccttct tcttggtggc cttcttgcgg gcgccggcct tcttcttggg
    17641 cgggttggtc tcccggcgga tctgcagcag ctccgccgcc cgctcgatgg tgatctcctc
    17701 gacgaggtcg cccttgcgca ggctggcgtt ggtctcgccg tcggtgacgt aggggccgaa
    17761 gcgtccgtcc ttgacgacca ccggcttctc gctcaccggg tcgtcgccca gctcgcgcag
    17821 cggcggcttc ggcgccgctt ggccgcgccg gcgcttgggc tcggcgaaga gcgccttggc
    17881 cttgtccatg tcgacggtga agagctcctc ctcggcctcg aggctgcggg catcggcgcc
    17941 cttctcgtcg cccttggaga tgtaggggcc gtaccgcccg ttgcgggcca gcaccggcag
    18001 accggtctcc tcgtcggtgc ccacgacccg tggcagcgac agcagccgca gcgcgtcgtc
    18061 gagggtgacc tcctcgggct tcatctcctt gaagagggac tccatcttcg gcttctcgtc
    18121 cggcgggagg gtgtcctggt cgccgagctg cacgtagggc ccgtaccggc cggtcttgac
    18181 gtacacgggc aggccggact cgggatgctc cccgatgggc tcgtcgccct tgggcgcggc
    18241 caggagctcg agcgccttgt cgagggtgag ctcgtcgggc gggaggcctt cggggatcga
    18301 cgccgtgtcg tcctcgccgc gcttgacgta ggggccgtac cggccgggct tggccacgac
    18361 catctcgccg ttctcgtcca tgccgagcgg gaaggtgttg acctcggccg cgtcgacctt
    18421 ctcgagggct tcgtccacca cgaccgagag ccccttgcgg gccccggggc caccgctggc
    18481 gccgtcgtcg ccgaaccaga agcgcttcag gtacgggacg cggtcctcct cgccgacggc
    18541 gatgaggtcg aggtcgtcct ccatcttggc ggtgaactcg tagtcgacgt actcgccgaa
    18601 ctggcgctcg agcacgttca ccacggcgaa ggccgtccag gtcggcacca acgccgtgcc
    18661 cttcttgaac acatagccgc ggtcctggat cgtggtcatg atcgaggcgt aggtggaggg
    18721 gcgaccgatg cccagctcct ccatgcgctt caccagcgac gcctcggtga agcgggcggg
    18781 cgggctggtg gtgtgcccgt cggcggtgag cgccggcgtg ggcagggcgt cgccctcggc
    18841 gaccgcgggc aggacggact cggcgtcgtc gcgctcggcg tcgtcgtcgt cgctgtcctc
    18901 gacgtaggcc ttgaggtagc cggggaaggt gatgacccga ccggaggcac cgaacgtgca
    18961 ctcgttggcg ggctggccgc cgtcggcgcg ggcctcggcc acgatgcgca tcgagatggt
    19021 gcgaccgacg gcgtcctcca tctgggaggc gacggtgcgc ttccagatga ggtcgtagag
    19081 gccgagctcg tccttgcgca gctcaccctt cacctggtcc ggcgtgcgga aggtctcgcc
    19141 ggccgggcgg atggcctcgt gggcctcctg ggcgttcttg gacttgccgg cgtactggcg
    19201 gggctggtcc gggacgtagt cggcgccgta gagctcgcgc acctggctgc gcgccgcgtc
    19261 gagcgcggtc tgcgacagcg tggtgctgtc ggtacgcatg taggtgatgt agcccttctc
    19321 gtagagaccc tgggcgatgc gcatcacctg gttggccgac atgcggagct tgcggccgcc
    19381 ctcctgctgg agggtggacg tcatgaacgg cggcttcggc cggctccgcc acggcttctc
    19441 gtcgatggag cggaccgtgt acggctgctc gtcgaggcgg gtgcgcaggc tggtggcggt
    19501 gggctcgtcg accacggcga cgtcgtcgcg ggtgatccgg ccgtcctggc cgaagtcacg
    19561 cccggtggcg acgcgcttgc cgtcgagggc cacgagccgg gcggtgaagg ccgggtcggt
    19621 ggggaaggca gcggtgaggt cccagtagtc ggcggcaacg aacgcgatgc gctcccgctc
    19681 gcgctcgacg acgaggcgga cggccgggct ctggacccgg ccggcggaca gcccgcggtt
    19741 caccttgcgc cacatgatct ggctgaccgg gtagccgtag agccggtcga cgatgcgacg
    19801 ggtctcctgg gcgtcgacgc gccggtagtc gatgtcgcgg gagttctcga ccgcgtgctc
    19861 gatggccgag cgggtgatct cgtggaacac catgcgcttc acggggacct tcggcttcag
    19921 cacctccttg aggtgccacg cgatggcctc gccctcgcgg tcctcatccg tcgcgaggta
    19981 gagctcgtcg gcgtccttca gcagcgcctt caggcgcttg atgacgtcct tcttgttcgg
    20041 gttgacctcg tactcggtct ggaagtcggc gtcgacgtcg atcgacaggc ccttcgaggg
    20101 caggtcgcgc acgtgcccga ccgatgcctc gacgatgtag tcgttcccga ggaactggct
    20161 gatcgtcttg gccttggcgg gggactcgac gatgacgagc ggcttggaca cggcagcagg
    20221 gtatgaacga cgtctcgcgg cgtgtcaagg cgccggtacc gtcccgaccg tggtcgacct
    20281 gctccgcttc gtgcaccgac tggcggacga cccggccgct cgggaccgga tccggcgcga
    20341 cccgcaggcc gtgctcgacg tcgaggtgga cgacgccgcc accctcacgg gcgaggacgt
    20401 cgaggccgtg gccgaggtgc tccggcgccg cctgccggcc gcggccgccg cgctcgtcga
    20461 gctccccgcc ccgtcacggc cggtgggttc ggagacgccg cgcgacgcgg cactccgtct
    20521 gctcatcggc ctgtgcgacg ccctcgacgg ccccgccgtc caaccggtca ccctccccca
    20581 ccgcgagctc cgacccgacc cgcccccgcc ccgtcagacg gcccggggcg gtggcgcacc
    20641 cgtgccgacc gaaccgcccg gcggtcgctg gctccacccg gtgatccccg acgacgaccc
    20701 ctgaaggagc gaactggcct acatcgacga ccaccaggtg gcatcgatgt aggccagaac
    20761 gggacggaac cggcgtcagc cgatgcgggt tccggcgcgc ccctcgatga tcgaggcgag
    20821 atcgtccagc gcaccgatca cggcggggtg cccggtggcg gtggcgaagc ggcaggcggc
    20881 gtcgaccttg ggacgcatcg agcccgccgg gaggtcgccc acgtcgatct ccgccggtgc
    20941 gatgcgatcg atgcggcgct ggtcgggcgt gccccacgcc tcgtacacgc cgtcgacgtc
    21001 cgtggccagg acgagcaggt cggcgccgag ctcccgggcg agcagtgagg aggcgaggtc
    21061 cttgtcgatc accgcttcga ccccgacgag gtgccgctcc ttgcccggct cgtacatcgt
    21121 ggggatccca ccgccgccgg cgcagatcac cacgcagcct cggtcgagca accaggagat
    21181 cggccggagc tcgaagatcc gcttcggttc gggcgaggcg accacccgcc ggaagtgctc
    21241 accgtccggc cggaccgtga accccttcga cgccacgagc gcgtcggcct ccgccgctgt
    21301 gtacaccggc ccgatgggct tcgtcggttc atcgaacgcc gggtcgtccg ggtcgacctc
    21361 ggtcatcgtc aagatggtgg ccagctggcg ctcgaagggc aggaggttgc ccagctcctg
    21421 ctcgatcatg tagccgatgc tgccttcggt cgcggcgccg aggacatcga ggggagacgc
    21481 ctcgtccggc cggtacgcct ccccctgcag tgccagcagc ccgacctggg gaccgttgcc
    21541 gtgcgagacc accagctcgt gctcgagcgc gaccggtgcg agcgcctcac acgccagccg
    21601 gacgttgagg cgctgcgccg ccgtcgtcat cggctcgccc cgacggagga gcgcgttgcc
    21661 cccgagggcg acgacgatcc gcacggctag ctcacgctcg ggggtccggg acctgctggg
    21721 tgatgcagtg gatgttgcca ccgccgagga ggacctcccg ggcgggcacc ccgacgatgt
    21781 cgcgatcggg gaaggccttg ccgtagacgt cgagcgcctc gtcgtcgacg cgctcgtcga
    21841 gcaggggcac cacgaccctt ccgttgcaca ggtaggcgtt ggcgtacgac gccgccaggc
    21901 ggtcgccagg gaggcggggc agcgtcccgt cgacggcgtc gacaccggcg gactcctcct
    21961 cggtgatgtg gaccggcccc ggggacggga gcgtcagcac ctcgatgctc cggccccggg
    22021 cgtcggtcca ggactcgagg cgtcggcgtg catcggcgga gatctcgtgc tgcggatcgg
    22081 acgcgtcgtc ggtccaggtg agcagaacca ggccgggccg tacgacgttg accaggttgt
    22141 ccacgtggcc gttggtctcg tcgaggtgga caccgcggtc gagccagagc accttctccg
    22201 cccgggtgta ggccacgagc tcgcgctcga tctccgcctt cgacagcccc gggttgcggc
    22261 cccggctgag caggcactcc tccgtcacga agagggtgcc ttcaccgtcg ctgtggatgg
    22321 atccgccctc gaggacgatc gtcgcccggt accggtcggc gccctcgatc tcgcagacct
    22381 tctgcgccac cgcgtcgtcg aggtcccacg ggaagtagag cccgtcgacc agaccacccc
    22441 aggcgttgaa gacccagtcc acgccgcgca gcccgccgcg atcgtcgacg acgaaggtcg
    22501 gtccgatgtc ccgcatccac gagtcgtcgt tggccatctc cacgacgcgg acgtgcgcag
    22561 gcaggctgga gcggcagttg gcgtactgcc gggccgacac cgccatcgtc accggctccg
    22621 ccgccgcgat ggccgtggcc accgcgacga acgcatcctg cgcgggcttc gcaccgagcc
    22681 gccagttgtc cgggcgctcc ggccagacca tccacgtgcc gaggtggcgc tcgaactccc
    22741 ccggcatgcg gaacccgtcc gctgccgggg tcgtccccag ggtggcagtc acgtctcggt
    22801 cccggcacgc cgagcccgga gggcccgtcc caccagcacc tcgcctgcgg ccagggtcgc
    22861 caccacgatg gcgaccagcc accccgtgta ggcccagtcc acgaccacgc ccggggtcca
    22921 caggaacagg accagcgacg ccacgatgca gaacgtggtg agcaccgtcc acgcgatgcc
    22981 gccgaccttg ccgcccggtg cccggtaggg gcgaggggtg tcggggtcga tcttccgcag
    23041 ccggaggaac gacgggaaca tcagcacgta cgggaggagg aacaccaccg acgacgccgc
    23101 gaagatggcg aagtagaggg tgtcctgctc ctccaggatg gcggtggtgc cgagcagcac
    23161 gaccgaggag atgacgccca tcgccacgaa cgcgaaggtc ggcgtccgcc gcaccgggtg
    23221 ctcccgcccg aacgctgccg gcagctcacc gtccgtggcc gcctccgctg cggctcggtt
    23281 ggcgcccatg ctccacgtcg tcatgttcgt gacgaacgtg tagaggaccg cgatgccgag
    23341 gccgtagacg gcgacgtcac ccgccgtcga ggtcccgaag atcgcctcga acgtgtcgac
    23401 cagccccgac accaggccga ggtcctgcat cgacagggcc atgaggatgc ccatggtgcc
    23461 gaagaagtac agcgcggcga tgatcacggc cgaccagccg aacgcgcgtg gcatgttccg
    23521 ctcggggtcc ttgacctctc cggccatgct ggagatgagc tcgaagccga gcagctggta
    23581 cacgagcacc ggcaggaaga acttcgccgc atcccagtcg ggaacgaaca tgtccttggc
    23641 ggccaggtcg ttggcagcgc cgtcgcggac cagcaggtag aagccgccca cggcgagggc
    23701 accgatgatg gtgatcttca gcacggcgcc caggttgttg acccacttgc cgatctcgag
    23761 cttctgcacg ccgatccaca ccacgagcca cgtgaggccg agggcgatgg cggcccgagg
    23821 ccacttgccg aggtcctcgg cgaagagctc ggcgaacgtg ccggcgaaga gcaggaacac
    23881 cgacggcatc cacagcgcga cgttcaccca gtaccagtag gtggtccggg ccgcccactt
    23941 cgcgccccac gcctgcttga tccagatgta gagaccgccc tggtcgggat aggtcgtggc
    24001 cagctcggcc gtgatgaagg cgctcgggat caggaagacc acgaacgcga cgatccacca
    24061 gccgacgacc gacggcccga tcgaagccga tgccgtcagc tggtcgacga cgagcatcgc
    24121 cgacacggtg aagagcgtga tgtcacgaag ccccatgacc cgccggcgcc cgctggcccg
    24181 atcggtcgtc atgggctcgt tcctgggctc cggggcgacc gtcgacgacg gctcccggtc
    24241 ggctgcttca cggggtggtt ggaggatgga catggtgtct cccgattctc ctcagtcggc
    24301 ccgtcggccg atgcggccca gctgctcccg gatgcggccg gcgtggagcg cctgcagctc
    24361 aggggagatg tcgaagtcga tgtccggcgc ggtggtccag acgaggatgg ccttctccgc
    24421 gtggagccgg ttctcggcct ggtcgaagat gacggactgg ggcgaatcgg ccacctcgtc
    24481 ggtcacctcg acgccccgcg aggccgggag gcagtgcatg aaccgggcat cggccggcgc
    24541 tcgggccatg agggcctcgt tcacctggta gcggggcatg aacgccgccc gccgctgcgg
    24601 gatctcgtcc tcctggtcca cccaccacca caggtccgtg tagacgaagt cggcctcgcg
    24661 cacggcgtcc tcgacctcgt cggtgacccg caccgatccg gagccggcgt cggccacgtt
    24721 ctgctcggcg agcttcaccc acgcctcggg cgactggtac gcggcgggac cggcgtggac
    24781 gaagtgcatc ccgagcttcg tggtgatgtg catcgtcgac gagcacacgt tcgtggcgtc
    24841 gccgacgaac accacgttgc agtccgagag cgccttgccc ggcgggaggt gctcgctcat
    24901 cgtgaagacg tcgcagatcg cctgcgtcgg gtggttgtag tcggtgaggc cgttgatgac
    24961 cggcacatcc gaccaccgcg ccagctcggt cacggtctcg tgcacgagcg tccgggccat
    25021 cacgacgtcg cacatgcgag cgagcacgcg ggccgtgtcg gccagcgact cgcgagcacc
    25081 gaggtggatc tcccccggct tcaggtagag cgcgtggccg ccgagcttca ccatcgcgac
    25141 ctcgaacgac acccgggtcc gggtcgaggg ctcctcgaag atcatcgcga gcgaacgtcg
    25201 gcggagcagc tcggggacgc aaccgtcacg gtcggcgtcc ttcagcaagc gggtgagctc
    25261 gagcacctcc tccagctccg cgcggctgaa gtcgttggtg tcgaggaagt gcctgcccat
    25321 ctgcggttgc tccgtcctgc cgccacctca gcggcgcagc gcggacggta cgaccgtgcc
    25381 cggcggatgg cccgggccca aggtcacctg cagccgggtc gctgctcccg aaacccgcac
    25441 cagaacgcga actggcctac cgattccccc gcatgcgggg gaatcggtag gccagaacgg
    25501 gtttgcggag gggtgctact tgatggtctt gacgatgatg gcgtcgccct ggccgccacc
    25561 gccgcagagg gcggcagcgc cgtactcgac gtcgcggcgc ttcatctcga gcagcagctg
    25621 cagcgcgagg cggttgccgg acatgcccac cgggtgaccg agggcgatgg cgccaccgtt
    25681 gacgttgacg atgtcgtcgg agatgccgag gtcctccatc gaggcgagac cgacggcggc
    25741 gaaggcctcg ttcagctcgt acaggccgag ctggtcctgc gacatgcccg agcgctggag
    25801 ggcgtccttg atggcccggc tcggctgggt gagcagcgac gggtcgggac cggcgacctg
    25861 gccgtagccg acgacctcgc cgaggggcgt gatgccgagc tcgtcggcct tggcccggga
    25921 gaccacgatg gtggcggagg cgccgtcgga gatctggctg gcgttgccgg cggtgatgtt
    25981 gccggccttg tcgaaggcgg gacggagctg ggcgagggac tcgccggtgg tgtcgccacg
    26041 cacgccctcg tcctcgctga ccatgaccgg gtcacccttg cgctggggca cggggaccgg
    26101 gacgatctcg tcgtcgaaca ggccgttctt cgcggccgcg gccgcacgct ggtggctctg
    26161 ggccgagaac tcgtcctggg gctggcgctc gaggccggcc gaggcggcgt acttctcggt
    26221 gccggcgccc atggcgcaca tgtcgaaggc gcagaacagg ccgtcgtgca tcatcgagtc
    26281 gacgaccttc tggtcgccga tgcggtagcc gccgcgggcg cccggcagca ggtacggggc
    26341 gttggtcatg gactccatgc cgccggcgac cacgatgtcg gcctgaccgg cctggatcat
    26401 gaggtcggcc aggtagatgg cgttgaggcc ggacaggcag accttgttga tggtggtggc
    26461 cgggacatcc atggggatgc cggcgttgaa cgcggcctgg cgggcggtga tctggccggc
    26521 gccggcctgg atcacctggc ccatgaccac gtagtcgacc tggtcgggag cgatcccggc
    26581 cttctcgagc gcgcccttga tggctacgcc cccgaggtcg gtggcggcca tcgaggcgaa
    26641 ggccccggac agcttgccga tgggcgtgcg ggcaccggtg aggatcacgg atccggacat
    26701 agggcaactc ccccgtcgat ggttgtgctg gtcggaacgg tagtgagaca ggcgtaccgg
    26761 gcggtaatgc ccgtcacacc aggtccttga ccgagtcggg catctcgtcg gcgacccgga
    26821 tgatcgggat gccggtctgg gtgttctcgt gcatctcctg gaagcaccgg ggcagatcct
    26881 cgaaggagta gatctcgtcg tggatcgtgg gcttgaagac cgagccgtac agctccgtcg
    26941 cggcctcgca cccctcgggg gtctcgtagt gggtgtggtc gatggtgacc tgcttcaccg
    27001 actgcaccgt ggagttgtag gagaccacct gggacagctg ccacccggcg ctgacgttca
    27061 cgccgagtcg agcctgcacg gcgaggccgg cctcgaagac cgggccgcgg agcatgtcgc
    27121 acacgaggtg catgccgacg ccgtcggtga ggcccttgac gtgcttgttg aacgccttga
    27181 cgtcgtccga ggaggcgaag cggttgaact gcttctggtc gatgccgacg atgccctgct
    27241 tctcgagcgc gtcgcggcgc tccttggagc cggcgcagaa gaacgcgttg tggccctcgg
    27301 ccttggccag catcaggaac agctcaccca cgccgccgcc gaagctgagc acgttgaggc
    27361 gggcctggcg ctcgtagggg accttgaccc ggtagatgcc gatggcgcgg cgccacaggt
    27421 ggtaggcggt cggcgcccgg agcgggagcg cggcgatctc ccagaggttc agcccggagt
    27481 cgagcggcgc cttgatgatc tgccagtcct cgaccacggc ctccttcgag taccagccga
    27541 tggagtcggg catgtcgtag gcccagatcc gcagcgggaa gccgaaccgg tccgggtcac
    27601 cgttgcagtg ggtgatgacg acgtcgcccg gggcgaacgc gctgacgtcg gcacccacct
    27661 cgatgacctc gccgacggcg gagttcccgg ggtacatctt gccgccgcgg gcctcggcga
    27721 tgttgatggt gtcggccgtg gcggcgtggt cgatgttgtg ctcggcggag accgcgagga
    27781 tcttcagctt gacgtcgtgc ggcccgaggt cgcgcagctc caggtcgtcg agctggacca
    27841 ccttggagac gtcgatggcg gcgatgtcgc ccccgttggc ctcggtctcg gcagcgaccg
    27901 cctcggcggt gatcgcgtag gccttggtca tcgtggtcat gtggcgtggt tcctccggtc
    27961 agctcggtta ccgaccggtc atcatgtcgg atcgggacgt cctgatggga ttcgggctgg
    28021 tccgatcgca ctacatttcg ggtcatgctg ctgaccgaga tcgaccacgt cgccatcgcc
    28081 gtcaaggacc tcgacgccgc catcgcctac tacgggaagg cgttcggcgc ggaggtccac
    28141 caccgtgagg tcgtggactc cgacggcgtc gaagaagcgc tggtcaaggt ggccgactcc
    28201 tacatccagc tcacggccgc cacccgcgac gactccccca tcgccaagtt cctggagaag
    28261 aagggcgagg gcctgcacca catcggctac cgggtcgacg actgcgccga ggccctcgag
    28321 tccatggtcg ccgccggcgc cacgcccatc gacaaggcgc cccgccccgg gtcgcgtggc
    28381 accaccgtgg ccttcatcca cccgaagggc agcctcggca ccct
//
LOCUS       DATISU010000089         1777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_50999, whole genome shotgun sequence.
ACCESSION   DATISU010000089 DATISU010000000
VERSION     DATISU010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50999"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VLR27_03845"
     CDS             <1..974
                     /locus_tag="VLR27_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02605.1"
                     /translation="RRAHPRVRRVWLASLVIDAVLVAGVLAAFSLRPAPAGAEPPADA
                     APLEHHVELGYGADVAPTETYPDGRIETGDTAFRTLTDDVDVFARTVGPDGVDVDGFV
                     QVDLDVSSSAGWSRTVSLVERTDLDGDLDLTAPIDFARFDGLAQRIAFETGVSAGTLD
                     LTVVLSGEANVADGEPVELEATLPLRLGPQTLTLTGAETTDTDDGPVVATTAELPAAQ
                     ATDDEPASDERPDLTRPALGALLLGVGITAVVWPTGQDEDDPDAVVRIRTTELYHPLS
                     ASSVEVADPADVEGIARHAHAPILCRDDGWQGVVNDDVLYWTPPPVP"
     gene            complement(1064..>1777)
                     /locus_tag="VLR27_03850"
     CDS             complement(1064..>1777)
                     /locus_tag="VLR27_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase PurH"
                     /protein_id="HSP02606.1"
                     /translation="ANPCGAAVADTLEEAYRLAFECDPRSAFGGIVATSQPIDDATVE
                     RMVGAAQADVVIAPGYDGGVVEALIAKRKNTRILTAPAPGKPGLALRQLNGAWLVQEP
                     HHFSSAQDEWRVVTKRQPTEAELADAAFAWRVGGWVKSNSIVLAKDGTAWGIGAGQQN
                     RVEAGEIAASKAAGRAEGGACASDAFYPFPDGIEAAAAAGAAVIIQPGGSVRDEECIA
                     KADELGVAMVFTGERHFLH"
BASE COUNT          237 a          702 c          606 g          232 t
ORIGIN      
        1 accggcgcgc ccacccccgg gtccgccggg tgtggctggc cagcctcgtc atcgacgccg
       61 tcctcgtggc cggcgtcctc gccgcgttct ccctccgccc ggcccccgcc ggcgccgagc
      121 cgccggccga cgccgcaccg ctcgagcacc acgtcgagct cggctacggc gccgatgtgg
      181 caccgaccga gacctacccc gacggccgca tcgagaccgg cgacaccgcc ttccgcaccc
      241 tgaccgacga cgtcgacgtg ttcgcacgca cggtcggtcc cgacggcgtg gacgtcgacg
      301 gcttcgtgca ggtcgacctc gacgtctcga gcagcgccgg ctggtcgcgc accgtctcgc
      361 tcgtcgagcg caccgacctc gacggcgacc tggatctcac cgctccgatc gacttcgccc
      421 gcttcgacgg cctcgcccag cgcatcgcgt tcgagaccgg cgtgagcgcc ggcaccctcg
      481 acctgaccgt cgtgctctcc ggggaagcca acgtcgccga cggcgagccg gtcgagctcg
      541 aggccacctt gccgctgcgc ctcggccccc agaccctcac gctcaccggc gccgagacca
      601 ccgacaccga cgacggcccg gtcgtggcca ccaccgccga actgcctgct gcccaggcaa
      661 ccgatgacga acccgcgtcg gacgagcgtc ccgacctcac ccggcccgca ctcggtgcgc
      721 tgctgctcgg cgtcggcatc accgccgtcg tgtggccgac cggccaggac gaggacgatc
      781 ccgacgccgt ggtccgcatc cgcaccaccg agctgtacca cccgctgtcg gccagcagcg
      841 tggaggtcgc cgacccggcc gacgtcgagg gcatcgcccg ccacgcccac gcgccgatcc
      901 tgtgccggga cgacgggtgg cagggcgtgg tcaacgacga cgtcctctac tggacgccgc
      961 cacccgttcc gtagccgcag ccagaaccgg acgcggctcc cccacccacc cgttcctcac
     1021 gcgcgtttcg accgtcaacg gtcggatcac gcgaagggaa cgatcagtgg aggaagtggc
     1081 gctcgccggt gaagaccatg gccacgccga gctcgtcggc cttggcgatg cactcctcgt
     1141 cgcggaccga accgcccggc tggatgatga ccgcggcgcc ggcggccgcg gcggcttcga
     1201 tgccgtcggg gaacgggtag aacgcgtcgg acgcgcacgc cccgccctcg gcccgtccgg
     1261 cggccttgga cgccgcgatc tcgccggcct cgacccggtt ctgctggccg gcgccgatgc
     1321 cccaggccgt gccgtccttg gccagcacga tggagttcga cttcacccag ccgccgacgc
     1381 gccaggcgaa ggcggcatcg gccagctcgg cctcggtcgg ctggcgcttc gtgacgaccc
     1441 gccactcgtc ctgggccgag gagaagtggt ggggctcctg caccagccag gcgccgttca
     1501 gctggcgcag ggcgaggccg ggcttgcccg gcgcgggcgc agtgaggatg cgggtgtttt
     1561 tgcgcttggc gatgagggcc tcgaccacgc cgccgtcgta gccgggagcg atgaccacat
     1621 cggcctgggc ggcgccgacc atccgctcga ccgtggcgtc gtcgatgggc tggctggtgg
     1681 cgacgatgcc gccgaaggcc gagcgcgggt cgcactcgaa ggcgaggcgg taggcctcct
     1741 ccagcgtgtc ggccacggca gcgccgcacg ggttggc
//
LOCUS       DATISU010000090         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_97504, whole genome shotgun sequence.
ACCESSION   DATISU010000090 DATISU010000000
VERSION     DATISU010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97504"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..978
                     /locus_tag="VLR27_03855"
     CDS             13..978
                     /locus_tag="VLR27_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSP02607.1"
                     /translation="MGKAADVADAVRAIEGVVTAEDVTGPYDVIARAESDDVDQLGRM
                     VVSKVQLIPGITRTLTCPVVRLEELVSSKRPGPTWWSGATAATSASTGTAAPSMSVPD
                     TSTVTRPGADVTCRTAPSVALATRPISSWTSARARTASAGGATAEPDHSRSIDAPISS
                     RAASPSLPTAARSSSSVSSSGEGTPGDGDTLTSHVRCAAATERPQSSAACSSLPLAAA
                     SASASATSAAARAAASCFIRLATSAASRRARLARSRASSRSRRASAAWPSARRRAESA
                     ALRARFASASSSVTDCRRAFVRSSSTPSPRATTRRTMATASTGRM"
     gene            complement(1001..1366)
                     /locus_tag="VLR27_03860"
     CDS             complement(1001..1366)
                     /locus_tag="VLR27_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02608.1"
                     /translation="MVGDLHASFAWFVVVGNGLAGAWALAAHWWEPARVRALWIATAV
                     VQVSIIVQVALGVAALGAIEGDPDQFHLFYGFVAVATVGIIYSYRAQLATHRYLLYGG
                     GGLFLMGLALRSMQFSQPA"
     gene            complement(1370..1690)
                     /locus_tag="VLR27_03865"
     CDS             complement(1370..1690)
                     /locus_tag="VLR27_03865"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HSP02609.1"
                     /translation="MTVIKINAITVPEGSGDELAQRFAARAGAVDGQEGFEGFELLKP
                     TDDRNVWLVVTRWADQASFDAWVSSPGFAHGHRGADKPTDSGAPVSVSAELWSYEVAG
                     GSAG"
     gene            complement(1748..>2384)
                     /locus_tag="VLR27_03870"
     CDS             complement(1748..>2384)
                     /locus_tag="VLR27_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HSP02610.1"
                     /translation="GIVCALLEAQRSGEGQVVDAAMVDGAAVLTTMFHAFRAMGIWND
                     ERGTNLLDTGAHFYDVYETSDAKYVSIGSIEPQFYAQLLELSGLGADEELPHQMDRGQ
                     WPALKERVAEIFKTKTRDEWCEIMEHTDVCFAPVLSLGEAPEHPHNVERRTFVEEHGV
                     TQPAPAPRFSRTEATIQRPPAHAGQHTDEVLGQFGFDADRITKLREAGAIA"
BASE COUNT          333 a          856 c          887 g          308 t
ORIGIN      
        1 atccagaccg aggtgggcaa ggccgccgac gtcgccgacg ccgtccgggc catcgagggc
       61 gtcgtcaccg ccgaggacgt caccggcccc tacgacgtga tcgcccgggc cgagtccgac
      121 gacgtcgacc agctcggtcg catggtcgtg tccaaggtgc agctgatccc gggcatcacc
      181 cggacgctga cctgccccgt cgtgcgcctc gaggagctcg tcagctcgaa gcggcccggt
      241 cccacctggt ggtcgggcgc gacagccgcc acgagcgcgt cgaccggcac ggccgcaccg
      301 tcgatgtcgg tgccggacac ctcgacggtg acccggcccg gcgcagacgt gacgtgcagg
      361 acggcgccgt cggtggcctt ggccacccgc ccgatcagct cctggaccag tgccagggcg
      421 cgcacggcga gcgccggcgg cgccacggcg gagccggacc actccaggtc gatcgacgcc
      481 cccatctcct cgcgagccgc gtcgccctcg ctcccgacgg cggcccgcag ctcgtcgtcg
      541 gtgtcgtcga gcggtgaggg cacgccgggc gacggcgaca cgctgacctc ccacgtgcgc
      601 tgcgcggcgg ccacggaaag gccccagagc tcggcggcct gctcgtcgct gccgctcgcc
      661 gccgcctccg catcggccag cgcgacctcg gcagccgccc gggcagcagc ctcctgcttc
      721 atccgcttgg ccacgtcggc ggcgtcgcga cgggcccggc tggcccgttc gagggcgtcg
      781 tcgcgctcgc ggcgggcgtc ggcggcctgg ccgtcggcga ggcggcgagc cgaatcggcg
      841 gcgctcaggg cccggttcgc ctctgccagc tcctcggtca cggactgcag gcgggcgttc
      901 gtccgcagca gcagcacgcc gagcccgagg gcgacgacga ggaggacgat ggcgaccgcg
      961 agcacgggcc gaatgtagtg agcgttcgga ccggcgccgt tcaggccggc tggctgaact
     1021 gcatcgaccg cagcgccagg cccatcagga acaggccacc gccgccgtag agcaggtagc
     1081 ggtgggtggc cagctgggcc cggtagctgt agatgatgcc gacggtggcg acggcaacga
     1141 agccgtagaa gaggtggaac tggtcggggt caccttcgat ggcgccgagg gcggcgaccc
     1201 cgagcgcgac ctgcacgatg atcgacacct gcaccacggc ggtggcgatc cacagcgccc
     1261 ggacgcgcgc cggctcccac cagtgcgcag cgagtgccca cgccccggcg agcccgttgc
     1321 ccaccaccac gaaccacgcg aaggacgcgt ggaggtcgcc gaccacggat cagccggcgg
     1381 aaccgccggc gacctcgtag ctccagagct cggccgacac cgacacgggc gcgcccgagt
     1441 ccgtgggctt gtcggcgccg cggtgcccgt gggcgaaccc gggcgaggac acccaggcgt
     1501 cgaaggaggc ctggtcggcc cagcgggtga cgacgagcca gacgttgcgg tcgtcggtgg
     1561 gcttgagcag ctcgaagccc tcgaagccct cctggccgtc gacggcacca gcgcgagcgg
     1621 cgaagcgctg ggcgagctcg tcgcccgaac cctcggggac ggtgatggcg ttgatcttga
     1681 tgacggtcac cggcccatcc tggcgtctgg ccccgcgtgc gagccagacg ccgggcgccg
     1741 gcggacgtca ggcgatggcg ccggcctcgc gcagcttcgt gatccgatcg gcgtcgaagc
     1801 cgaactgccc gagcacctcg tcggtgtgct ggccggcatg ggccggcgga cgctggatgg
     1861 tggcctcggt gcggctgaag cgcggggccg gcgcgggctg ggtgacgccg tgctcctcga
     1921 cgaacgtgcg gcgctcgacg ttgtgggggt gctcgggggc ctcgcccagg ctgagcaccg
     1981 gcgcgaagca cacgtcggtg tgctccatga tctcgcacca ctcgtcgcgg gtcttggtct
     2041 tgaagatctc ggcgacccgc tccttcagcg cgggccactg gccacggtcc atctggtggg
     2101 gcagctcctc gtcggcgccg aggccggaga gctcgagcag ctgggcgtag aactgcggct
     2161 cgatcgagcc gatcgacacg tacttggcgt cgctggtctc gtacacgtcg tagaagtggg
     2221 cgccggtgtc gagcaggttc gtgccccgct cgtcgttcca gatgcccatg gcccggaagg
     2281 cgtggaacat cgtggtgagc acggcggcac cgtcgaccat ggcggcgtcg acgacctggc
     2341 cctcaccgga gcgctgggcc tcgaggagcg cgcagacgat gccg
//
LOCUS       DATISU010000091         3729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_702958, whole genome shotgun sequence.
ACCESSION   DATISU010000091 DATISU010000000
VERSION     DATISU010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702958"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="VLR27_03875"
     CDS             complement(<1..1404)
                     /locus_tag="VLR27_03875"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HSP02611.1"
                     /translation="MSTIASTADVIRTHAKERGDKVAIIQDEHRQTWAELDQRSNRVA
                     NALAAAGVGEEDRVAFIDKNGYEYFEVFFACAKLNAVTVNVNWRLAPPEVAWIVNDAE
                     AKVFVFGQEFADTVAAIRDDLSSTSTFVCLGDDDRFESYEAWMSDDDSDPGVQPSGAD
                     VAFQLYSSGTTGLPKGVMLSNDNLFALLPDASEQWGFSESMVSMVAMPLFHIGGSGWA
                     MAGMFNGGTSVIVREIDPAAIIDIIGKERITHSFLVPAVFQFMLMMPNVDDGDYSSLE
                     MLVYGASPITEDVLAASVKKFGCKFAQAYGLTETTGAIVTLPPEDHDVDGPNRHRLRS
                     AGKPNGTVEMKIVDPDTFEEVPTGEVGEIITRSNQNMVGYWKNEEGTQSAYVDGDWFR
                     TGDIGYFDDDGYLYIHDRVKDMIVSGGENVYPAEVENALMKHPGIADVAVIGVPDEKW
                     GEVGKALVVKAEGQDPDP"
     gene            complement(1479..2153)
                     /locus_tag="VLR27_03880"
     CDS             complement(1479..2153)
                     /locus_tag="VLR27_03880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSP02612.1"
                     /translation="MSADSAPVVLFDVDGTLTDTNYLHTLAWRRAFLDNGHDVASWRI
                     HRLIGASGTKLMEDCIGRADEDVKSAWRTHFEELAAEVKAFPGARALIELVQERGGKA
                     VLATSSPEDLVAHHLQALGLDEADLGGITTDTDVEEAKPAPDVFLTALAKAEGDATRA
                     IVIGDTGWDLQAARAAGISAVAVRTGGWGHDELVQAGAVEVHDDVGTLCSDLETSVLG
                     DLLRRA"
     gene            2186..3394
                     /locus_tag="VLR27_03885"
     CDS             2186..3394
                     /locus_tag="VLR27_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSP02613.1"
                     /translation="MDLDVALVAIGGLGVVVVFASRLIRFVPLTAPLLALALGVVLGP
                     EVLDLASFDEGPTILHAASEIVVAVALMAVALRFPWSEVRELLGPVGWMVTVGMVAMA
                     AIVAGLAWAVLGVGLGAAVLLGGVLAPTDPVLSSSVVTGDPATRTLPHRVRGLLSVES
                     GINDGLALPLVVAGTVLVLDDGLGRFAVEGVLAVAIAVVLGAALGTGAGECFRRLDEA
                     HDIEDSAFFVFTLVLAVAVLGVVNLAHGDGILAVYVAGLAYNRQVGTSIYEQEREVDE
                     GINRVLVLPLFLLLGVLLPWEAWGDLGWPVVTFTVGVLVLRRLPVVLAMRPLLGLRWS
                     SAAFYGWFGPMGAAALFFATLAHEEHATADVVWPVTALVVAASTLVHGATAAPLRMLY
                     ERVEGARGDG"
     gene            3425..>3729
                     /locus_tag="VLR27_03890"
     CDS             3425..>3729
                     /locus_tag="VLR27_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HSP02614.1"
                     /translation="MPSYIESDGYERDTSYIPTRITADGRDGYPVEPGRYRLVVARAC
                     PWANRSIIVRRLLGLEDVLSMGVCGPTHDERSWTFDLDPGEKDPVLGIERLQEAYFAR
                     "
BASE COUNT          460 a         1304 c         1341 g          624 t
ORIGIN      
        1 cgggtcgggg tcctggccct cggccttgac gaccagggcc ttgccgacct cgccccactt
       61 ctcgtcgggc acgccgatga cggcgacatc cgcgatgccc gggtgcttca tcagggcgtt
      121 ctcgacctcg gccgggtaca cgttctcgcc gccggagacg atcatgtcct tcacccggtc
      181 gtggatgtag aggtagccgt cgtcgtcgaa gtacccgatg tcgccggtgc ggaaccagtc
      241 gccgtcgacg taggccgact gggtgccctc ctcgttcttc cagtacccca ccatgttctg
      301 gttggagcgg gtgatgatct cgccgacctc gccggtgggc acctcctcga aggtgtcggg
      361 gtcgacgatc ttcatctcga cggtgccgtt gggcttgccg gccgagcgca ggcggtgccg
      421 gttggggccg tcgacgtcgt ggtcctcggg cggcagggtg acgatggcgc ccgtggtctc
      481 ggtcaggccg tacgcctggg cgaacttgca gccgaacttc ttcaccgacg cggccagcac
      541 gtcctcggtg atgggcgagg cgccgtagac gagcatctcg aggctcgagt agtcgccgtc
      601 gtcgacgttg ggcatcatca gcatgaactg gaacacggca ggcaccagga acgagtgcgt
      661 gatgcgctcc ttgccgatga tgtcgatgat ggcggcgggg tcgatctccc ggacgatgac
      721 cgacgtgccg ccgttgaaca tgccggccat ggcccagccg gagccgccga tgtggaacag
      781 cggcatggcg accatgctca ccatcgactc gctgaagccc cactgctcgc tggcatcggg
      841 cagcagcgcg aacaggttgt cgttgctgag catcacgccc ttgggcaggc cggtggtgcc
      901 cgacgagtag agctggaagg ccacgtcggc gccggagggc tgcaccccgg ggtcgctgtc
      961 gtcgtcgctc atccacgcct cgtaggactc gaagcggtcg tcgtcgccga ggcacacgaa
     1021 cgtggaggtg gaggacaggt cgtcgcggat ggcggcgacg gtgtcggcga actcctggcc
     1081 gaacacgaac accttggcct cggcgtcgtt cacgatccac gccacctcgg gcggggccag
     1141 gcgccagttc acgttgaccg tgaccgcgtt cagcttggcg caggcgaaga acacctcgaa
     1201 gtactcgtag ccgttcttgt cgatgaacgc gacgcggtcc tcctcgccga ccccggccgc
     1261 cgcgagggcg ttggccacgc ggttggagcg ctgatcgagc tcggcccacg tctggcggtg
     1321 ctcgtcctgg atgatggcga ccttgtcgcc ccgttccttc gcgtgcgtac ggatgacgtc
     1381 ggctgtgctg gcgatggtcg acatcgcatg ttccccctgg atcggatgcg ttcgggggca
     1441 catcctgcca atcgggggag ggtcgcgcac tgcgcccctc aggcgcgacg cagcaggtcc
     1501 cccaggaccg aggtctcgag gtcgctgcag agcgtgccga cgtcgtcgtg cacctccacg
     1561 gcgccggcct ggaccagctc gtcgtggccc catccgcccg tgcggaccgc cacggcgctg
     1621 atgccggcgg cgcgcgcggc ctggaggtcc cagcccgtgt cgccgatgac gatcgcccgg
     1681 gtcgcatcgc cctcggcctt cgccagggcg gtgaggaaca cgtccggcgc cggcttggcc
     1741 tcctccacgt cggtgtcggt cgtgatgccc ccgaggtcgg cctcgtcgag gccgagcgcc
     1801 tgcaggtggt gcgccacgag gtcctcgggc gacgacgtgg ccagcaccgc cttgccaccc
     1861 cgctcctgga ccagctcgat gagggcacgg gcaccgggga aggccttgac ctccgcggcg
     1921 agctcctcga agtgggtgcg ccacgccgac ttcacgtcct cgtcggcccg gccgatgcag
     1981 tcctccatga gcttggtgcc cgacgcgccg atgaggcggt ggatgcgcca cgacgccacg
     2041 tcgtgaccgt tgtcgaggaa ggcccggcgc caggccaggg tgtgcaggta gttggtgtcg
     2101 gtcagggtcc cgtcgacgtc gaagaggacc acgggggcgg agtcggcaga catggggccg
     2161 tccctacccg ggtagtgcgt cggcgatgga cctggatgtc gcactcgtcg cgatcggcgg
     2221 cctcggcgtc gtcgtggtgt tcgcatcccg gctcatccgc ttcgtgcccc tgacggcacc
     2281 gctgctcgcc ctcgcgctcg gcgtcgtgct gggacccgag gtgctcgacc tggccagctt
     2341 cgacgagggc ccgacgatcc tgcacgcggc cagcgagatc gtggtggcgg tggcgctcat
     2401 ggccgtcgcg ctgcggttcc cctggtccga ggtgcgcgag ctgctcgggc cggtgggatg
     2461 gatggtgacg gtcgggatgg tcgccatggc cgcgatcgtc gctggcctcg cctgggcggt
     2521 gctcggcgtc ggcctgggcg cggcggtgct gctcggcggc gtgctcgccc ccaccgaccc
     2581 ggtcctgtcg tccagcgtcg tgaccggcga cccggccacc cgcaccctcc cccatcgcgt
     2641 ccgcgggctc ctctccgtcg agtcgggcat caacgacggc ctcgccctcc cgctggtggt
     2701 ggccggcacc gtgctcgtcc tcgacgacgg cctcgggcgc ttcgccgtcg agggcgtcct
     2761 cgccgtcgcc atcgccgtcg tcctcggcgc cgcgctcggc accggtgccg gcgagtgctt
     2821 ccggcgcctg gacgaggccc acgacatcga ggactcggcg ttcttcgtgt tcaccctcgt
     2881 gctcgccgtg gccgtgctcg gggtcgtcaa cctggcccac ggcgacggca tcctcgccgt
     2941 gtacgtcgcc ggcctcgcct acaaccgcca ggtgggcacc tcgatctacg agcaggagcg
     3001 cgaggtcgac gaggggatca accgggtgct ggtcctgccg ctgttcctgc tgctcggggt
     3061 gctcctgccc tgggaggcgt ggggtgacct cggctggccg gtcgtgacct tcaccgtcgg
     3121 ggtgctggtg ctgcgccggc tgcccgtggt gctggcgatg cgaccgctgc tcggcctgcg
     3181 ctggtcgagc gcggcgttct acggctggtt cggtccgatg ggcgccgccg ccctgttctt
     3241 cgccaccctc gcccacgagg agcacgccac ggccgatgtg gtgtggcccg tgaccgcgct
     3301 cgtcgtcgcg gccagcaccc tcgtgcacgg ggcgacggcc gcgcccctcc ggatgctcta
     3361 cgagcgggtc gagggcgccc ggggcgacgg gtgacggccc gcccgcaggg gtaggactga
     3421 ggccatgccc tcctacatcg agtccgacgg gtacgagcgc gacacgagct acatcccgac
     3481 gcgcatcacc gccgacgggc gcgacggcta cccggtcgaa ccgggccgct accgcctggt
     3541 cgtcgcccgg gcgtgcccgt gggcgaaccg gtcgatcatc gtgcgccggc tgctcgggct
     3601 cgaggacgtg ctgtcgatgg gcgtgtgcgg gccgacgcac gacgagcgca gctggacctt
     3661 cgacctcgac cccggggaga aggacccggt gctcggcatc gagcggctcc aggaggcgta
     3721 cttcgcccg
//
LOCUS       DATISU010000092         4635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_516795, whole genome shotgun sequence.
ACCESSION   DATISU010000092 DATISU010000000
VERSION     DATISU010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516795"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VLR27_03895"
     CDS             complement(<1..594)
                     /locus_tag="VLR27_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HSP02615.1"
                     /translation="MAKILKFGDDARRSLEAGVNRLADSVKVTIGPKGRNVVLEKKFG
                     APTITNDGVSIAREVELEDPFENMGAQLVKEVATKTNDIAGDGTTTATVLAQSLVHEG
                     LRNVAAGASPMQLKKGIEAAVKAAVDSIAEQANEIDDKSEIAQVAAISAADPTIGEIL
                     ADAIDKVGKDGVVTVEESNTFGMDLEFTEGMQFDKGYL"
     gene            complement(610..900)
                     /gene="groES"
                     /locus_tag="VLR27_03900"
     CDS             complement(610..900)
                     /gene="groES"
                     /locus_tag="VLR27_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HSP02616.1"
                     /translation="MNLQPLEDRIVVRPSESEETTASGLVIPDTAKEKPQQGEVLAVG
                     PGKRSEQSGELIPVDVSVGDTVVYSKYGGTEIASGGEDLLILNARDVLAIVK"
     gene            complement(1003..1395)
                     /locus_tag="VLR27_03905"
     CDS             complement(1003..1395)
                     /locus_tag="VLR27_03905"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HSP02617.1"
                     /translation="MSDHVDDAQDLTGVLPIQQPRLADLLEGDPPLPHAVLVVLSGPA
                     RGTTLVVQSLPAELGRAPEADLVLDDDTVSRRHAVLRGGREALEIEDLGSSNGTNING
                     NPMAGALSLLDGDLVTFGSATCLVKRIG"
     gene            complement(1424..1909)
                     /locus_tag="VLR27_03910"
     CDS             complement(1424..1909)
                     /locus_tag="VLR27_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02618.1"
                     /translation="MSDARLDIDDVDLESLVLARNDVHPDGDWWFDPRTGESLYFGID
                     DDEDLAQLVRGVHVLIPGEPQPREDIDDFLVSDDLASVGEDVVVRLLEAYRGRGGLRR
                     FREVVTRTPAAEVWGRFMLAREGSRAIRWLLERDLVDEDSARRRLQELAGSASAGADT
                     E"
     gene            1976..2254
                     /locus_tag="VLR27_03915"
     CDS             1976..2254
                     /locus_tag="VLR27_03915"
                     /inference="COORDINATES: protein motif:HMM:NF025379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HSP02619.1"
                     /translation="MGLLNNVKKLASKENIAKAKGVAAKNADKITGTVEKATSTIDQK
                     TGGKYRDKLDKVEDTVARNLEKAKDEGDDPGTGTVDGPPPGGPAGPNR"
     gene            complement(2261..3295)
                     /gene="tsaD"
                     /locus_tag="VLR27_03920"
     CDS             complement(2261..3295)
                     /gene="tsaD"
                     /locus_tag="VLR27_03920"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HSP02620.1"
                     /translation="MTQPTRDLSTVRTVLGIESSCDETAAAVVRDGQVQLSSVISSQI
                     DRHARFGGVVPEIASRAHVEMLNPVIAEAIVQAGIADDEIDAVAATYGPGLVGSLLVG
                     VSAAKALALVWDVPFVAVNHLEAHLYSAMLEDPTFELPVVVLLVSGGHTMLVHWEAEG
                     SYRVLGGTIDDAAGEAYDKVARYLGLGYPGGPVIDRISLDGDPESIAFPRALPDRPYD
                     FSFSGLKTSVVNHVRKHPDVSAEDVAASFQEAVVDVLVTKTRRAAADVGAKGISIGGG
                     VAANSRLREQILTVCAEDGLAAYLPDRANCTDNAAMVAAAGWWQLQRAGESPLDTGAD
                     PGLGLGSPAA"
     gene            complement(3292..3846)
                     /gene="rimI"
                     /locus_tag="VLR27_03925"
     CDS             complement(3292..3846)
                     /gene="rimI"
                     /locus_tag="VLR27_03925"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HSP02621.1"
                     /translation="MQELDDLHAASAPVRIGPMRRRHLRGVLRIEELANPRPWSLSLF
                     LSELRYTESRAYLIARVGTEIVGYAGLMLVAGDGHVTNVGVDPDHRRRGIATRLMLEL
                     VRRGLEEGAEALTLEVRVSNQAAQELYRRFGFVPAGVRKNYYSDANEDALIMWATDID
                     TPDYRARLQSIEQALPTPTLREAT"
     gene            complement(3855..4571)
                     /gene="tsaB"
                     /locus_tag="VLR27_03930"
     CDS             complement(3855..4571)
                     /gene="tsaB"
                     /locus_tag="VLR27_03930"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HSP02622.1"
                     /translation="MLILGISTSTKQVGCAIGGHEGVLASSHSARGTRHAENLAPAID
                     FVRRQARIELDEISVVAVDIGPGLFTGLRVGIAAAKAMAHALRVPMIGISSLDLLAWP
                     VRHTSREIVAVIDARRGEVFYARYRPTPGGVQRLGEPVVATPDDLRADLEATDSDYLV
                     VGDGGLRYADELDSIIRVEVAEQHLAHPNAASLVQLAHAKAIREDFVQPWQLEPLYLR
                     PPDAEINWSTREGLTPGEAR"
BASE COUNT          670 a         1558 c         1728 g          679 t
ORIGIN      
        1 caggtagccc ttgtcgaact gcatgccctc ggtgaactcg aggtccatgc cgaaggtgtt
       61 ggactcctcg acggtgacca cgccgtcctt gccgaccttg tcgatggcgt cggcgaggat
      121 ctcgccgatg gtcgggtcgg cagcggagat ggcggcgacc tgagcgatct cgctcttgtc
      181 gtcgatctcg ttggcctgct cggcgatgga gtcgaccgca gccttgacgg cggcctcgat
      241 gcccttcttg agctgcatcg gggaggcgcc ggcggccacg ttgcggaggc cttcgtgcac
      301 cagcgactgg gcgagcacgg tggcggtggt ggtgccgtcg ccagcgatgt cgttggtctt
      361 ggtggccacc tccttcacca gctgggcacc catgttctcg aacgggtcct cgagctcgac
      421 ctcacgggcg atggacacgc cgtcgttggt gatggtcggt gcgccgaact tcttctcgag
      481 cacgacgttg cggcccttgg ggccgatcgt caccttcacg gagtcggcca ggcggttgac
      541 gccggcctcg agggagcggc gagcgtcgtc gccgaacttc aggatctttg ccatgtgtga
      601 gtcttttcct tacttgacga tggcgagcac gtcgcgggcg ttcaggatca ggagatcctc
      661 gccgccggag gcgatctcgg tgcctccgta cttgctgtag acgacggtgt cgccgacgga
      721 gacgtcgacg gggatgagct caccggactg ctcggagcgc ttgccggggc cgacggccag
      781 gacctcgccc tgctggggct tctccttggc ggtgtcgggg atgaccaggc cgcttgcggt
      841 ggtctcttcg gactcgctcg gacggacgac gatgcggtcc tcgaggggct gcaggttcat
      901 cggggacgcc tccatgggtg acgcgttagc actcggtacg gacgagtgct aacggtaacg
      961 gactcacctg gcactcgcaa gacgagactg ctaatcgggc gctcagccga tccgcttcac
     1021 caggcacgtc gccgacccga aggtgacgag gtcgccgtcc agcaggctca gcgccccggc
     1081 catcgggttg ccgttgatgt tggtgccgtt ggaggacccg aggtcctcga tctcgagcgc
     1141 ctcgcggccg ccgcgcagca cggcgtggcg gcgggacacc gtgtcgtcgt cgagcaccag
     1201 gtcggcttcg ggcgcccggc ccagctcggc gggcagcgac tgcacgacga gcgtcgtgcc
     1261 ccgggccggg ccgctcagca ccaccagcac ggcgtgcggc agcggcgggt cgccctccag
     1321 caggtccgcc aggcgcggct gctggatcgg gagcaccccg gtcagatcct gtgcatcgtc
     1381 gacgtgatcg ctcacagcac tcctcggtcg ggcgagactc ccgtcactcc gtatccgctc
     1441 ccgcgctcgc tgaccccgcc agctcctgga ggcgtcgacg ggcggagtcc tcgtcgacca
     1501 ggtcgcgctc gagcagccac cggatggcgc ggctgccctc gcgggcgagc atgaagcgac
     1561 cccacacctc ggccgccggg gtgcgggtga ccacctcccg gaaccggcgc agcccgcccc
     1621 gccccctgta cgcctcgagc aggcggacca cgacgtcctc accgaccgag gcgaggtcgt
     1681 cggacaccag gaagtcgtcg atgtcctccc gcggttgggg ttcaccgggg atcaggacgt
     1741 gcacgccccg caccagctgg gccaggtcct cgtcgtcgtc gatgccgaag tagaggctct
     1801 cgccggtgcg cgggtcgaac caccagtcgc cgtcggggtg gacgtcgttc cgggcgagga
     1861 cgagcgactc caggtcgacg tcgtcgatgt cgaggcgggc gtcggacacg gcgccgacgg
     1921 taccccggtg ccggcatggg taagggtgga gggcacccga gcgaaggaga cccccatggg
     1981 cctgttgaac aacgtgaaga agctggcctc gaaggagaac atcgccaaag ccaagggcgt
     2041 ggcggcgaag aacgccgaca agatcaccgg cacggtcgag aaggcgacgt ccaccatcga
     2101 tcagaagacc gggggcaagt accgcgacaa gctcgacaag gtcgaggaca ccgtcgcccg
     2161 caacctcgag aaggccaagg acgagggcga cgatcccggc accgggaccg tggacggccc
     2221 gcccccgggt ggaccggccg gcccgaaccg ctagcccggc tcaggcggcg ggcgacccca
     2281 ggccgaggcc cgggtcggca ccggtgtcga ggggcgactc gcccgcccgc tgcagctgcc
     2341 accacccggc cgcggcgacc atcgccgcgt tgtcggtgca gttggcgcgg tcggggaggt
     2401 aggcggcgag gccgtcctcg gcgcacacgg tgaggatctg ctcccgcagc cgcgagttgg
     2461 ccgccacgcc accgccgatg ctgatgccct tggcgccgac gtcggccgcc gcccgtcgcg
     2521 tcttggtcac caggacgtcg acgacggcct cctggaacga ggcggcgacg tcctcggcgc
     2581 tcacgtcggg gtgcttgcgc acgtggttca cgaccgaggt cttgagcccg gagaaggaga
     2641 agtcgtaggg ccggtcgggc agcgcccggg ggaaggcgat ggactcgggg tcgccgtcga
     2701 ggctgatgcg gtcgatgacc ggaccgccgg ggtagccgag tccgaggtag cgggcgacct
     2761 tgtcgtaggc ctcgccggcg gcgtcgtcga tggtgccgcc gagcacccgg tagctgccct
     2821 cggcctccca gtgcacgagc atggtgtggc cgcccgacac gagcagtacc acgaccggca
     2881 gctcgaaggt ggggtcctcc agcatcgccg agtacaggtg ggcctcgagg tggttcaccg
     2941 ccacgaaggg cacgtcccac accagggcca gcgccttggc cgcggagacg ccgaccagca
     3001 gcgaacccac caggccgggg ccgtaggtgg cggcgacggc gtcgatctcg tcgtcggcga
     3061 tgccggcctg gacgatggcc tcggcgatga cggggttgag catctcgacg tgggcgcggg
     3121 acgcgatctc gggcacgacc ccgccgaagc gggcgtggcg gtcgatctgg ctggagatca
     3181 ccgacgagag ctgcacctgg ccgtcgcgga cgacggcggc cgcggtctcg tcgcaggagc
     3241 tctcgatgcc gagcacggtg cgcacggtcg acaggtctcg ggtgggctgg gtcatgtcgc
     3301 ctcgcggagg gtgggggtgg gcagcgcctg ctcgatggac tggagccggg cgcggtagtc
     3361 gggggtgtcg atgtcggtcg cccacatgat cagggcgtcc tcgttggcat ccgagtagta
     3421 gttcttgcgc acgccggcgg gcacgaagcc gaagcgccgg tacagctcct gggccgcctg
     3481 gttcgagacc cggacctcga gggtgagcgc ctcggcgccc tcctccagcc cgcggcggac
     3541 cagctcgagc atcagccggg tggcgatgcc ccgacggcgg tggtcggggt cgacgccgac
     3601 gttggtgacg tggccgtcgc cggccaccag catcagcccg gcgtacccga cgatctcggt
     3661 gccgacccgg gcgatcaggt aggcccgcga ctcggtgtag cgcagctccg acaggaacag
     3721 gctgagcgac cacggtcgcg ggttggccag ctcctcgatg cgcagcacgc cccgcaggtg
     3781 acggcggcgc atgggtccga tgcggacggg ggccgaggcg gcgtgcaggt cgtcgagctc
     3841 ctgcatgacc atggtcagcg ggcctcgccg ggcgtgaggc cctcgcgggt gctccagttg
     3901 atctcggcgt cgggtgggcg caggtacagg ggctcgagct gccagggctg gacgaagtcc
     3961 tcgcggatcg ccttggcgtg ggccagctgc acgagcgagg ccgcgttggg atgggccagg
     4021 tgctgctcgg ccacctcgac ccggatgatg ctgtcgagct cgtcggcgta gcgcaggccg
     4081 ccgtcgccga cgacgaggta gtcgctgtcg gtggcctcga ggtcggcgcg caggtcgtcg
     4141 ggcgtcgcca ccaccggctc gccgaggcgc tgcaccccac ccggcgtcgg gcggtagcgg
     4201 gcgtagaaca cctcgcctcg gcgggcatcg atgacggcca cgatctcccg ggacgtgtgg
     4261 cgcaccggcc aggccagcag gtcgaggctg gagatgccga tcatcggcac ccgcagcgcg
     4321 tgggccatcg ccttggcggc agcgatcccg acgcgcagac cggtgaagag cccgggaccg
     4381 atgtcgaccg ccaccaccga gatctcgtcc agctcgatgc gggcctggcg gcggacgaag
     4441 tcgatggccg gggccaggtt ctcggcgtgg cgcgtgcccc gcgccgagtg cgaggaggcg
     4501 agcacgccct cgtggccgcc gatggcgcag ccgacctgct tggtcgaggt ggagatcccg
     4561 aggatgagca tctaggcctc cgcccagggc tggagcttct cgccgagcgt gcggtgccgc
     4621 gccgaccacc gaccg
//
LOCUS       DATISU010000093        20804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_563519, whole genome shotgun sequence.
ACCESSION   DATISU010000093 DATISU010000000
VERSION     DATISU010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 20804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20804
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563519"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /gene="cofC"
                     /locus_tag="VLR27_03935"
     CDS             complement(<1..565)
                     /gene="cofC"
                     /locus_tag="VLR27_03935"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HSP02623.1"
                     /translation="MAAPPIAVVVPVKDFTRAKVRLAEHLDAAARAELARDMAGIVLA
                     AAAPLPVSVVCDDVDVRAWAESAGADVIWTPGLGLNGAVQAGVAELADRGVETVVVAH
                     SDLPLATSLAWVAATPGVTLVPDRRLDGTNVLAVPAAAGFRFAYGAASFDRHRAEGLR
                     LGLPVRIVRDARLGWDVDHPADLALPAH"
     gene            635..1648
                     /locus_tag="VLR27_03940"
     CDS             635..1648
                     /locus_tag="VLR27_03940"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HSP02624.1"
                     /translation="MSSINVAVLGGGSWGTTVAHLCAHKAPTTLWARSEETVADINGS
                     HRNGRYLPDYDLHPSLRATTDLPEALGTADLVVMGIPSHAFRETLREAVPHIRPWVPL
                     VSLTKGLELDTGLRMTEVVHEELPGHPVGVLTGPNLAKEIMAGAATASVLSMQDDSVA
                     QALQELFGGEAFRVYTNDDLIGCELGGVFKNVIAIASGMADGMGTGDNTRAALITRGL
                     AEITRIGVAYGGSPMTFAGLAGMGDLIATCISPQSRNRTVGQKLGEGMAIDAIITEMN
                     QVAEGVKSCRAVQSMAREAGVEAPITDQVVAVCHEGASAQEVYARLLGRASRPELHGI
                     ADR"
     gene            1645..4011
                     /locus_tag="VLR27_03945"
     CDS             1645..4011
                     /locus_tag="VLR27_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02625.1"
                     /translation="MSVRTWLVRRLLGRLRIDDDTIRHLSTFQRLGEHLEIQAPGEML
                     PIGARTVFRALRTRAASQTGTDWVWPYWLRRQIDPSSPAFVPRGHLPFLSNVTSRDWT
                     MIGNRGAAREAVVDPRGLVSPWHDAWSLDWWIGAEDRWHVPADEANVRQSLLGDSPVV
                     ETRMRVPGGDVIHRAYAIQATAADGGEELVIVEIENDSAVPVALALAIRPYNAEGLAV
                     IERIGLHDTTVTVDGRVAMLLPKPPNAVAASTFHDGDCATQVFAGETQTSFPSDLRDE
                     AGMAQAAFVFPLPHRQTLRVALPLAPDPRTRLVGRARKRVRDAPRFPQAVPSAEQVAS
                     GWKAHTDQGMRLVLPDERLQSAVDANRRYLLLFHDGDDVTPGPRTYNRFWFRDAAYLL
                     AALDRYGFHTEAREVLASFPGRQHADGFFFSQRLEWDANGAALWALAHHWDLTRDRAL
                     LDETITSVAGGVQWIDRKRRGKRRDDPALRGLLPAGISAEHLGPHDYFYWDDFWALAG
                     LRDGARLLRAADEDEAADRATSFLEAMTADVRASMALTAERLGTKAVPAGPRRRIDPG
                     VIGSLAAVWPLQLLGAEDEAMQATLDVIRERFCIDAAFYQGISHTGLGTYLTLQVAFV
                     ELLAGDRRALDRLDWFLSAATPTWTWPEAIHPRVAGGCMGDGHHGWAAAEFLNFVRTM
                     LVHETSDGLALASMIPASWYGQGVEVHDAPTRFGTLSFAIRWHGERPAILWELEPHDP
                     DVAVVLTCPGLDPTWRSTEARGEALLAAPPDAPAPAAAPGPDQGDSFG"
     gene            complement(4111..4917)
                     /locus_tag="VLR27_03950"
     CDS             complement(4111..4917)
                     /locus_tag="VLR27_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HSP02626.1"
                     /translation="MQELLRVAPGTGLDLSAIDTRSTPGFDGDKDEGKSLLHDRRTRL
                     ADLQDLLYAEGTNRLLVVLQAMDTGGKDGTIRHVFRKVDPIGVHMWGFKKPSSAELAR
                     DYLWRVHAKVPADGEVVVFNRSHYEDVLVVRVHGLIDDERAERRLAHIRDFERMLTEE
                     GTTIVKLFLHISKEEQAERLRSRLNRPDKHWKYSSADLAERAHWDRYQEVYRDAIAAT
                     STEEAPWYVVPADRKWYRNLAISTILVETLEGLDMSHPEPEEGLADVVVE"
     gene            4994..5854
                     /locus_tag="VLR27_03955"
     CDS             4994..5854
                     /locus_tag="VLR27_03955"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HSP02627.1"
                     /translation="MGDAPWTDLDITADPAAPGCFRATISDPWMLAVVPQGGIVAALA
                     VRAMSELLGDPTQTPRTTTAVFAGKVALGPVEIDARVLRRGRSMSQLTATVRNPGEEA
                     GLTAIAAFGAPRRGFSFTDLEMPDVDGPDGLRSFRDPLPEGVEFEFDRPPMPFWEQII
                     ESRPVIGRPPWEPFVACRAESANWVRIDEPPVLPDGRLDPLAALVVCDTMPGAVGQKL
                     GPDAGPWFGPSVDLTVHLLEPTEPGWLLAHNRARHAGDGYASVDQALWTEDGRLVAYA
                     TQLMFFAFGV"
     gene            complement(5915..8191)
                     /locus_tag="VLR27_03960"
     CDS             complement(5915..8191)
                     /locus_tag="VLR27_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HSP02628.1"
                     /translation="MPVPTRRTRALTAVAVAAALGMALAPTSSASLDPITSVVEEAFA
                     PQLVTVDTPTRADKDRLNALGLDLTEHAGHDYVEVVLHTPEDLLALTTAGFTYDVRIP
                     DLLARQLERIALDEAFAAATVRSSLPSGRDSYRTLPDFAAEIDGLVASKSDIAKRISI
                     GKSVEGRDLTGIEIGEGVNDPEDGRPVFLMFGAHHAREWPSAELPMEFAYDLVNGYGT
                     DPQITDLLQRGRAIIVPVSNPDGYDASRTSGDLVDLREIDNGGTVTILGTPGNAYKRK
                     NCRYIDGQTQPAGTCVAVPSPGGFGLGVDLNRNYGALWGGPGTSDNPADPVYRGPAPF
                     SEPETQAIRTLISNRQVTTVITNHTFSNLILRPVGVKPSATAPDGNPVGFAPDECFTE
                     ADGVDNGMQALGERMAAQNGYSNQFGWELYDTTGTTEDYSYNATGGYGYTFEIGPDEF
                     HPPYEHVVAEYTGDTAAAQGVTPENASELTTDAADECGGAHQGHDEVGGGNRAAFLEA
                     FENAVDAGTHSLITGQAPAGATLKLERTGVFPLWDGSKVADTVATTMTTTSTGAFAYH
                     ANPSTRPFVQSRKATALGEPKDTLSTSGSAAPLSSSDTPFEVTETADVLTATLTHTVP
                     SGDDTIRNMDLVLLDPSGAEVARTDEFAQVSNLAWTGPDGQGVPAGTYTLRVENTAGL
                     VPAYELEAATADVLPVSTERQFEQWTLTCITADGVAGQQGVSVERGQSVDVGDVCNAG
                     LGTVQPGNNGKKPNKGKR"
     gene            8538..8936
                     /locus_tag="VLR27_03965"
     CDS             8538..8936
                     /locus_tag="VLR27_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HSP02629.1"
                     /translation="MSGLAIITEACIDVKDRACVDVCPVQCIYEFDATTNQLFSEEEA
                     GSGIVENTHTPNPDAIAVFGDSMLYVNLEECTSCTACYQPDVCPVGAIYSEEQVPDGS
                     ATTAKYNAEDPHEGHDHTFFVQHSRAVFAD"
     gene            complement(8974..9729)
                     /locus_tag="VLR27_03970"
     CDS             complement(8974..9729)
                     /locus_tag="VLR27_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02630.1"
                     /translation="MTSDSEVAAEGIEEGPLLNTMMNLARFHRDHERFYAAAPRESAL
                     HIQRQARALQSLADRWSNVEVGDSAALSPFEGAEDLNDPAALQLEGVLFMEGEGRPAE
                     LTAMIRDLRTHAEDCRNIGEWLTQAMQASWQAVRALLDIDELADVLGERHRIISNDWL
                     AASMNTLIAELLDRAADMIDRIDFAPAAVRADLTGQRVLPRRLYSAAELLARAADLLS
                     ESAGLVHDNERRWRVFHRCVSEVVDATTTSPRA"
     gene            complement(9906..10424)
                     /locus_tag="VLR27_03975"
     CDS             complement(9906..10424)
                     /locus_tag="VLR27_03975"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HSP02631.1"
                     /translation="MLRKLRGRAASMKTIAALLTRAEEIARTRGTERPAAEHLVLAAL
                     QLPDGTATRALERLGFSAADFGAALDAQAVEDLERIGVHADDDGISAELPPPDEPVGI
                     YRSAPSAQELFQAAGEDARRGGGALVGAHVLRAAADLENGPTARALRRMGIDRSALRV
                     AATAEIEERSRS"
     gene            complement(10426..10668)
                     /locus_tag="VLR27_03980"
     CDS             complement(10426..10668)
                     /locus_tag="VLR27_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSP02632.1"
                     /translation="MQGTPDTIEVPDPSDPEAAFASVVALRRLADRLELATVNVAVRQ
                     GWSWTDVAAALGVSRQAAHKKFARRVERPDTRPRRD"
     gene            complement(10733..10927)
                     /locus_tag="VLR27_03985"
     CDS             complement(10733..10927)
                     /locus_tag="VLR27_03985"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HSP02633.1"
                     /translation="MAVTGTLERPRRGQGRVIAGVCAAIADRFGVSRTFVRLLFVIFG
                     LVGAGEVAYLVLWVLVPKRD"
     gene            11018..12316
                     /locus_tag="VLR27_03990"
     CDS             11018..12316
                     /locus_tag="VLR27_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSP02634.1"
                     /translation="MAKAPATPQEVIDLLEQVHDVCGRLEPGNVPLHVAPQVFDALAS
                     AERLIGGAVLRMAGRYEEAGEWKRNGAKSAEDDIARKTGTPTGTARKKLSTSKRLRNQ
                     QRTDEAIRNGDLSTEQANEVSSGADASPEDEDDLLDTARRHRLAELRKRAADARAKAD
                     RDREDRRRRHQGMRSVRRWTDEDGMGNLCLRLPPEDMAEVDAALKPRIDRAFADARQA
                     GRFEPVERYAADVVRDLLTGATDRTPARRNQAVRPDKKVIALIDLAALNRGSIEGDET
                     CEIAGVGPVSVSAVRALLHDAFLAVVIKDGIDILHVTHLGRRVTATQRTALEARSGGA
                     CEVCGSRYLLDIDHNEGWTLTHETRLDDLSWFCWHCHRTKTRHDLRVEGPVGARWFVD
                     RKTGKRWRPPPDEPREPPPGAGGAGDSGDPAQAELFTLAD"
     gene            12360..13079
                     /locus_tag="VLR27_03995"
     CDS             12360..13079
                     /locus_tag="VLR27_03995"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HSP02635.1"
                     /translation="MAPGTATTLRPSLKRKAPGTRRERELWELGHQVVVGIDEVGRGA
                     WAGPLTLGAVVIPKDKRLTKVRDSKMLTEREREVMYERIVGWADAWSVGHAWPEECDE
                     LGMSEAQRLAARRAIEGLGVVPDQVLLDGNWDFVGSGNVTKIVKGDATCLSIAAASIV
                     AKVTRDRLMRECADAHPWFSFETNKGYPCPRHKTALAGVGPSAIHRRSWVFMEHLPWA
                     GAVRSVPLHLRPDDSQGTLFA"
     gene            13076..13558
                     /locus_tag="VLR27_04000"
     CDS             13076..13558
                     /locus_tag="VLR27_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02636.1"
                     /translation="MTDARDAELARLLSEVRSLRLRPDDWDEVDGHLAAIEAGDRGRI
                     DDLSQAVFEARVRGRFSGPRGSAGVAPTKQTSVLPIVGLVCGGLLVGVGALLGGGPIL
                     WAIVALGVFVFGIAFAGSRVAHRPRGGADEEDTVEPVPIPDHVALRVNAIGRAHGAGR
                     "
     gene            complement(13570..13875)
                     /locus_tag="VLR27_04005"
     CDS             complement(13570..13875)
                     /locus_tag="VLR27_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02637.1"
                     /translation="MLQCTPAAAATLEAVRQQNDIPDGHGVRLFPAPAPEGEVGLGID
                     FAAEPQADDVVTEQHGTTLMVDGQINEQLDGLTLDVVPDPTQDGNGAPQLVLRAPEG"
     gene            complement(13940..14647)
                     /locus_tag="VLR27_04010"
     CDS             complement(13940..14647)
                     /locus_tag="VLR27_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HSP02638.1"
                     /translation="MESFAPTQPVATLDAEERGAFVIRVYQHLLLAIGAFVAFETVLF
                     QTGIAEAMHDFLVGASSAWLLILGAFMVIQWMATSAAHNIENEGLQYAGLFGMAAGQA
                     LIFAPFLYLVFNDPSNGSTSVAAAAAITAFGFAGLTVVAFVTRKDLSFLRPMLMWGGI
                     AALVLIVAAVLFGLSLGIWFSVAMIALAGGAILYQTQQIMARYPSHAHVGAAVQLFAS
                     VMMLFWYVLRLVSQLRR"
     gene            14731..15150
                     /locus_tag="VLR27_04015"
     CDS             14731..15150
                     /locus_tag="VLR27_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02639.1"
                     /translation="MDPVRMRPAVVLAAFTLLVWTTRIRNIWTDDSLSTTGQLGRTAL
                     SLSFTVFAAAVLWLWWRGRRAGSVDPIAGPVVRMLGAWTIGVWAVRVVQISTAGHDAA
                     FVAVHTALAVVSTVLAVWAVREVRRGSDAGSQRLRNG"
     gene            15162..15935
                     /locus_tag="VLR27_04020"
     CDS             15162..15935
                     /locus_tag="VLR27_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HSP02640.1"
                     /translation="MTEPLLLLASVGEAFGEVNDVFPSSEAVWRVLAAVALGAIVGVE
                     REVNDQPAGLRTHLTVALGAAVFGVISTLGFSEFVTDQRDVNVQFDVTRVASQVVVGI
                     GFLGAGMIFRSGSKVRNLTTAASLWVTAAIGLAAGVGDIGIAIVATAALLFALMLLRI
                     PRDAIRNRFTRSTAHVSIRVMGTAAAEPVLDAVRAIDGIAVLAVGWGKHEGDTVLDLT
                     LEGDAKIKPARHLAPIVARDDVISVNTDVFLGSEISDDA"
     gene            complement(15948..17816)
                     /locus_tag="VLR27_04025"
     CDS             complement(15948..17816)
                     /locus_tag="VLR27_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HSP02641.1"
                     /translation="MAAEPTPGRPRTGMLALGALGVVFGDIGTSPLYAFREAFEGHGH
                     ELDVLESNVLGVLSLVFWSLVIVISVKYLALVMRADSHGEGGILTLTSLVTPAQQRGG
                     RWVLVLLGLFGTALLYGDGMITPAISVLSAVEGTTIAAPALADLVLPITIAILIAIFS
                     VQRHGTHVVGRVFGPIMVLWFAVLGVLGARQILAAPRVLEALDPLHGVRFFADNGLTG
                     ILVLGAVFLVVTGGEALYADMGHFGHRPIAIAWFAVALPGLVLNYFGQGALLLEDPSA
                     IDSPFYRLAPDWALVPMVVLATAATVIASQALISGAFSLTMQAVQLGYAPRLRIVHTS
                     GSAQGQIYVPTVNWVLMVACIGLVLGFRSSSSLAAAYGIAVTTTMVITTVLFAVVART
                     LWEWSLGRVLAIAGGLLVIDLAFFAGNVPKIPAGGWFPIVAAAVLFALLTTWSTGRRL
                     VRDRIQLGRTPLVTYLAGLADDPPARVPGTAVYLFSIPGMTPPSLVANVRYNRVLHER
                     VVVASVVTDDVPRIEGRSRLRERQMGDGVSRVDVHYGFMEDPDLARDLAGVEGFHADE
                     ATYFLGKETVIATKRPGMAIWRERLFGVMGRNATSAADYFRLPPDRIFEVGTRVEI"
     gene            complement(17830..18663)
                     /locus_tag="VLR27_04030"
     CDS             complement(17830..18663)
                     /locus_tag="VLR27_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HSP02642.1"
                     /translation="MTQLGWGRRFLLCQPRHFGVLYEINPWMERGVAVDVDAALEQWH
                     GLHDALVEAGAEVETIEQPEGVPDLVFTANAGLVDATAKRFIPSHFRHPEREPETEVF
                     AEWFDDAGWEVTRLPEEVVHEGAGDALPFGGVLVSGYRPRSDLAAAPHLTGLLDVPVR
                     TLELVDERLYHLDLTFCPLDDRRAMCAPLGWDTYGRRLVESLVPEPLWLTDDEALAFC
                     ANSVVVGDVIHMPTCPPRVGRQLEAWGFHVVVCPVDEFLKAGGGCRCLTFALDIELSR
                     P"
     gene            complement(18660..19880)
                     /locus_tag="VLR27_04035"
     CDS             complement(18660..19880)
                     /locus_tag="VLR27_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HSP02643.1"
                     /translation="MATETIQVQGHIVDSLILAKILDAILEAGADYELTDVVIGKTNT
                     DSSRASIAVHAADQATLDALLDDLQVHGANRIAQSEAVLEAAEQDGVLPTGFHSTTNL
                     DTDVLIDGTWHTVENPEMDCGIVVDEPDGQLRVRTIPMHRVRAGDRIVVGRDGVRVRP
                     PERRAVAGTFEFMNSEVSSEKPKGLMIRQVAERIRAAREEGGRVLLVAGPAAIHTGAG
                     PDLAKLVEHGWISCLFTGNGFATHDLESNVLGTSLGVGVADGVPVEGGHANHVRVINE
                     IRRHGSIAAAVDAGYVTGGVMHACVRAGVPFVLAGSVRDDGPLPDTMTDVAEAADAMR
                     DQVPGVQVALMLATTLHAIATGNILPASVETFCVDINQAVVTKLADRGSHQAVGIVTD
                     IGVFVRQLTGELCS"
     gene            19960..20472
                     /locus_tag="VLR27_04040"
     CDS             19960..20472
                     /locus_tag="VLR27_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02644.1"
                     /translation="MGQSVNVVAKHSSNPEILRFEINRSLTGMGHERYRSLDDLVLDR
                     PVDRLARRLLEHGGVDGVHVNSSVITVDLGGGYVGDGLQQIIEDLFRFYPDAPDGNDA
                     PEADEASTEVAAEDAPVAQEPLTGDETELREAPEAAAATGDGELDTPAEAPAEAPDPD
                     RPDDEGPPAD"
     gene            20475..>20804
                     /locus_tag="VLR27_04045"
     CDS             20475..>20804
                     /locus_tag="VLR27_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HSP02645.1"
                     /translation="MVDAADWSLRFHRRLLAGDRFGATSVIEASLTAGTDPVGVYLDV
                     VAPAMREIGEGWAAGTIGVAEEHRAAVIVGQVLAVLDSRFSRRSGRRGLVVAGAVEGE
                     RHSLPLRI"
BASE COUNT         2917 a         7340 c         7562 g         2985 t
ORIGIN      
        1 agtgcgccgg cagcgcgagg tcggcggggt ggtcgacgtc ccagccgagg cgggcgtcgc
       61 ggacgatgcg caccggcagc cccagccgca ggccctcggc ccggtggcga tcgaaggagg
      121 cggcgccgta ggcgaaccgg aacccggcgg ccgcaggcac ggccagcacg ttggtgccgt
      181 cgaggcgtcg gtcgggaacc agggtgaccc ccggggtggc ggcgacccag gccagggagg
      241 tggccagcgg caggtccgag tgggcgacga cgacggtctc cacgcctcga tcggccagct
      301 cggcgacgcc ggcctggacg gcaccgttca ggccgaggcc gggcgtccag atgacgtcgg
      361 cgccggccga ctcggcccac gcccgcacgt cgacgtcgtc gcagaccacc gagaccggca
      421 ggggtgcggc tgcggcgagc acgatgccgg ccatgtcgcg ggcgagctcg gcccgggcgg
      481 cggcgtcgag gtgctcggcg aggcgcacct tggcccgggt gaagtccttg accgggacga
      541 cgacggcgat cgggggtgcg gccatgcacg gcgcagtgta gggacgcccg tcctcgctcg
      601 ggccgcccgg gaccctctca ctagggtgcg atccatgtcg tcgatcaacg tcgctgtcct
      661 cggcggcggt tcgtggggga ccacggtcgc ccacctctgc gcccacaagg cgcccaccac
      721 cctctgggct cgctcggagg agacggtcgc cgacatcaac ggttcgcacc gcaacggtcg
      781 ttacctgccc gactacgacc tgcacccgtc gttgcgcgcc accaccgacc tgcccgaggc
      841 actgggcacc gccgacctgg tggtgatggg catcccctcg cacgcgttcc gcgagaccct
      901 gcgcgaggcc gtgccccaca tccgcccgtg ggtgccgttg gtcagcctca cgaagggcct
      961 cgagctcgac accggcctgc gcatgaccga ggtcgtgcac gaggagctgc ccggccaccc
     1021 cgtcggcgtg ctcaccggcc ccaacctggc caaggagatc atggccggcg ccgccacggc
     1081 gtccgtgctc tcgatgcagg acgactcggt cgcccaggcc ctgcaggagc tgttcggcgg
     1141 cgaggccttc cgcgtctaca ccaacgacga tctgatcggg tgcgagctcg gcggtgtgtt
     1201 caagaacgtc atcgccatcg cctcgggcat ggccgacggc atgggtacgg gtgacaacac
     1261 ccgggctgcc ctcatcaccc gtggcctggc cgagatcacc cgcatcggcg tcgcctacgg
     1321 cggttccccg atgacgttcg ccggcctcgc cggcatgggc gacctcatcg ccacctgcat
     1381 ctccccccag tcccgcaacc gcacggtggg gcagaagctg ggggagggca tggcgatcga
     1441 cgccatcatc accgagatga accaggtcgc cgagggcgtg aagagctgcc gtgcggtgca
     1501 gtccatggcc cgcgaggccg gcgtcgaggc tccgatcacc gaccaggtcg tggccgtgtg
     1561 ccacgagggc gccagcgcgc aagaggtgta cgcgcgcctg ctgggccggg ccagccgccc
     1621 cgagctgcac ggcatcgcag accggtgagc gtgcgcacct ggttggtgcg gcgcctgctc
     1681 ggccgcctcc gcatcgacga cgacaccatc cgccacctct cgacgttcca gcgcctcggc
     1741 gagcacctcg agatccaggc gccgggcgag atgctgccca tcggggcccg caccgtcttc
     1801 cgggccctgc gcacccgggc cgccagccag accggcaccg actgggtgtg gccctactgg
     1861 ctgcgccgcc agatcgaccc gtcctcgccg gcgttcgtgc cccgcggcca cctcccgttc
     1921 ctgtccaacg tcaccagccg ggactggacg atgatcggca accgcggcgc ggcacgcgag
     1981 gcggtggtcg acccgcgcgg cctggtctcg ccctggcacg acgcctggtc cctcgactgg
     2041 tggatcggcg ccgaggaccg ctggcacgtc ccggccgacg aggccaacgt gcgccagtcc
     2101 ctcctcggcg actccccggt cgtcgagacc cggatgcggg tgccgggcgg cgacgtcatc
     2161 caccgggcct acgccatcca ggccacggcg gccgacggtg gcgaggagct ggtgatcgtc
     2221 gagatcgaga acgactccgc cgtcccggtc gccctcgccc tcgccatccg cccgtacaac
     2281 gccgaaggcc tcgcggtcat cgagcgcatc ggcctgcacg acacgacggt caccgtcgac
     2341 ggccgggtgg cgatgctgct cccgaagccg ccgaacgcgg tcgccgcgtc caccttccac
     2401 gacggcgact gcgccaccca ggtgttcgcc ggcgagacgc agacgtcgtt cccgagcgac
     2461 ctgcgggacg aggccggcat ggcccaggcc gcgttcgtct tcccgctgcc gcaccgccag
     2521 acgttgcggg tggcgctccc gctcgcgccc gacccccgca cccgcctcgt cggccgggct
     2581 cgcaagcggg tccgcgacgc gccccgcttc ccccaggccg tgccctccgc cgagcaggtc
     2641 gccagcgggt ggaaggccca caccgaccag ggcatgcgcc tggtgctgcc cgacgagcgg
     2701 ctgcagtcgg cggtcgacgc caaccggcgc tacctcctcc tgttccacga cggcgacgac
     2761 gtgacgccgg ggccgcgcac ctacaaccgc ttctggttcc gcgacgcggc gtacctgctc
     2821 gccgccctcg accgctacgg gttccacacc gaggcccgcg aggtgctcgc cagcttcccg
     2881 gggcgtcagc acgccgacgg cttcttcttc tcccagcgcc tcgagtggga cgccaacggt
     2941 gctgcgctgt gggccctggc ccaccactgg gacctcactc gcgaccgcgc gctcctcgac
     3001 gagaccatca cgtcggtcgc cggtggcgtg cagtggatcg accgcaagcg tcgaggcaag
     3061 cgccgggacg atcccgctct gcgcggcctg ctgccggcgg gcatctcggc cgagcacctc
     3121 ggcccgcacg actacttcta ctgggacgac ttctgggcgc tcgccggcct gcgcgacggg
     3181 gcccgcctgc tgcgcgccgc cgacgaggac gaagccgccg accgagccac ctcgttcctg
     3241 gaggccatga ccgcggacgt ccgggcctcc atggccctca cggccgagcg gctcggcacg
     3301 aaggcggtgc ccgccggtcc tcggcgccgg atcgaccccg gcgtcatcgg ttcgctggcc
     3361 gccgtgtggc cgctgcagct gctcggcgcc gaggacgagg ccatgcaggc gaccctcgac
     3421 gtcatccgtg agcggttctg catcgacgcc gccttctacc agggcatcag ccacaccggg
     3481 ctcggcacct acctgacgct gcaggtggcc ttcgtcgagc tgctcgccgg cgaccggcgt
     3541 gcgctcgacc ggctcgactg gttcctgtcc gccgccaccc cgacctggac ctggcccgag
     3601 gccatccacc cccgcgtcgc cggcggctgc atgggcgacg ggcaccacgg ctgggccgca
     3661 gccgagttcc tcaacttcgt gcgcacgatg ctcgtccacg agaccagcga cgggctcgcg
     3721 ctcgcgtcga tgatcccggc cagctggtac ggccagggcg tcgaggtcca cgacgcaccc
     3781 acgcgcttcg gcacgctgtc gttcgccatc cgctggcacg gagagcgtcc ggcgatcctg
     3841 tgggagctcg agccgcacga ccccgacgtg gccgtcgtgc tcacctgccc cggtctcgat
     3901 cccacgtggc gctccaccga ggcccgcggc gaggccctcc tggctgctcc tcctgacgcc
     3961 ccggcgccgg cggccgcgcc cggtcccgac cagggcgaca gcttcggttg actgcagatc
     4021 tgcgagatcg gagcgacctg ccgtgcggac ttggcggctc cgccgccgta gggcccgtga
     4081 catgggtcgc ctcgcctgtc ggctcggctc ctactccacg acgacgtccg ccaggccctc
     4141 ctcgggctcg ggatgggaca tgtcgagccc ctcgagggtc tcgaccagaa tcgtgctgat
     4201 ggccaggttc cggtaccact tgcggtcggc gggcacgacg taccagggcg cctcctcggt
     4261 cgaggtcgcg gcgatcgcgt cccggtacac ctcctggtag cggtcccagt gggcccgctc
     4321 ggcgaggtcg gccgaggagt acttccagtg cttgtcgggg cggttcaggc gtgaccgcag
     4381 ccgctcggcc tgctcctcct tgctgatgtg caggaagagc ttgacgatgg tggtgccctc
     4441 ctcggtgagc atgcgctcga agtcgcggat gtgggccaac cggcgctcgg cccgctcgtc
     4501 gtcgatcaga ccgtgcaccc gcaccaccag gacgtcctcg tagtggctgc ggttgaacac
     4561 gacgacctcg ccgtcggccg gcaccttggc gtgcacccgc cacaggtagt cccgggccag
     4621 ctcggccgag gacggcttct tgaagcccca catgtgcacg ccgatggggt cgaccttccg
     4681 gaagacgtgg cggatcgtgc cgtccttgcc gcccgtgtcc atcgcctgca gcacgacgag
     4741 caggcggttg gtgccctcgg cgtacagcag gtcctgcagg tccgccaggc gcgtgcgccg
     4801 gtcgtggagg agtgacttgc cctcgtcctt gtcgccgtcg aagcccggcg tggaccgggt
     4861 gtcgatggcg gacagatcga gaccggtccc gggtgcgacg cgcaacagct cctgcatgcc
     4921 ggccttccta cccggacgcg ccggcggaaa cccggccctc cccacgagca ccggctggtt
     4981 accgtcgccc gacgtggggg acgcaccgtg gacagacctg gacatcactg cggatccggc
     5041 ggcgccgggg tgcttccggg cgacgatctc ggacccctgg atgctcgccg tcgtgcccca
     5101 gggcggcatc gtcgcggcgc tcgccgtgcg ggcgatgtcc gagctcctcg gcgacccgac
     5161 ccagacccca cgcacgacca ccgcggtgtt cgccggcaag gtcgcgctcg gtcccgtcga
     5221 gatcgacgct cgggtcctgc gccgtggacg gtcgatgtcg cagctgaccg ccaccgtccg
     5281 gaacccgggg gaggaggccg gtctcacggc catcgccgcg ttcggcgcgc cccgccgggg
     5341 gttctccttc accgacctgg agatgccgga cgtcgacggc cccgacggcc tgcgcagctt
     5401 ccgtgacccg ctgccggagg gcgtcgagtt cgagttcgac cggccgccca tgccgttctg
     5461 ggagcagatc atcgagtcgc gtcccgtgat cggccggccg ccgtgggagc ccttcgtggc
     5521 gtgtcgcgcc gagtccgcca actgggtgcg gatcgacgag cccccggtcc ttcccgacgg
     5581 gcggctggat cctctcgccg cgctcgtcgt ctgcgacacg atgcccggag cggtcggcca
     5641 gaagctcggc cccgacgcgg gcccgtggtt cggcccgagc gtcgacctca ccgtgcacct
     5701 gctcgagccg accgagccgg gctggctgct cgcgcacaac cgggctcgcc acgccggcga
     5761 cgggtacgcg tcggtcgacc aggcgctgtg gaccgaggac ggccgcctcg tcgcctatgc
     5821 cacccagctg atgttcttcg ccttcggcgt ctgagcccgg acgcggcgag gccccgcgcc
     5881 gtgcgcgggc ctcgtccgtg cgtgcagctg gttgctagcg cttgcccttg ttgggcttct
     5941 tcccgttgtt gccgggctgg accgttccga ggccggcgtt gcagacgtcg ccgacgtcga
     6001 ccgactggcc gcgctccacg ctgacgccct gctgaccggc gacgccgtcg gcggtgatgc
     6061 aggtgagcgt ccactgctcg aactgtcgct cggtgctcac gggcaggacg tccgcggtgg
     6121 cggcctcgag ctcgtaggcg ggcaccaggc cggcggtgtt ctccacccgg agggtgtagg
     6181 tgccggcggg tacgccctgg ccgtcggggc cggtccacgc caggttgctc acctgggcga
     6241 actcgtccgt gcgggcgacc tcggcgccgg agggatcgag caggacgaga tccatgttgc
     6301 ggatcgtgtc gtcaccggac gggacggtgt gggtcagcgt ggccgtgagc acgtcggcgg
     6361 tctcggtgac ctcgaagggg gtgtccgagc tgctgagcgg tgcggcgctg ccgctggtgg
     6421 acagcgtgtc cttcggctcg cccagggccg tggccttgcg ggactgcacg aagggccgcg
     6481 tcgacgggtt ggcgtggtag gcgaaggcgc cggtcgacgt ggtcgtcatc gtggtggcca
     6541 cggtgtcggc gaccttggag ccgtcccaca gggggaacac gccggtgcgc tcgagcttca
     6601 gggtggcgcc ggcgggggcc tgcccggtga tcagcgagtg cgtgccggcg tcgaccgcgt
     6661 tctcgaaggc ctcgaggaag gcggcgcggt tgccgccacc gacctcgtcg tggccctggt
     6721 gggcgccgcc gcactcgtcg gccgcgtcgg tggtcagctc ggatgcgttc tccggcgtga
     6781 cgccctgggc cgctgccgtg tcgccggtgt actcggcgac gacgtgctcg tacggcgggt
     6841 ggaactcgtc cgggccgatc tcgaaggtgt agccgtagcc gccggtggcg ttgtagctgt
     6901 agtcctcggt cgtgccggtg gtgtcgtaga gctcccagcc gaactggttg ctgtagccgt
     6961 tctgggcggc catgcgctcg ccgagcgcct gcatgccgtt gtcgacgccg tcggcttcgg
     7021 tgaagcactc gtcgggtgcg aagccgaccg ggttgccgtc gggtgccgtg gcgctgggct
     7081 tcacaccgac cgggcgcagg atcaggttcg agaacgtgtg gttggtgatg accgtggtga
     7141 cctggcggtt cgagatcagc gtccggatcg cctgcgtctc cggctcggag aagggtgcgg
     7201 ggccgcggta gacggggtcg gcggggttgt cgctcgtgcc gggaccgccc cagagggcgc
     7261 cgtagttgcg gttgaggtcg acgccgaggc cgaagccacc cggcgacggg acggcgacgc
     7321 acgtcccggc cggctgggtc tggccgtcga tgtagcggca gttcttgcgc ttgtaggcgt
     7381 tgccgggggt gccgaggatg gtcacggtgc cgccgttgtc gatctcgcgc aggtcgacga
     7441 ggtcgccgga cgtgcgggag gcgtcgtagc cgtcggggtt ggagaccggg acgatgatgg
     7501 cccggccgcg ctggagcagg tcggtgatct gcgggtcggt cccgtagccg ttcaccaggt
     7561 cgtaggcgaa ctccatgggg agctcggcgg acggccactc acgggcgtgg tgggcgccga
     7621 acatgaggaa gaccggtcga ccgtcctcgg ggtcgttgac gccctcgccg atctcgatgc
     7681 cggtgaggtc gcggccctcg acgctcttgc cgatcgagat gcgcttggcg atgtcggact
     7741 tgctggcgac gagcccgtcg atctcggcgg cgaagtcggg cagggtgcgg taggagtcgc
     7801 ggcccgaggg gagcgacgag cgcaccgtcg ccgcagcgaa cgcctcgtcg agggcgatgc
     7861 gctccagctg gcgggcgagc aggtcgggga tgcggacgtc gtaggtgaag ccggcggtcg
     7921 tcagggccag caggtcctcg ggggtgtgga ggacgacctc gacgtagtcg tgaccggcgt
     7981 gctcggtgag gtccaggccg agggcgttca gccggtcctt gtcggcccgg gtcggggtgt
     8041 cgacggtgac gagctgcggg gcgaaggcct cctcgaccac cgaggtgatg ggatcgaggg
     8101 acgcgctgct ggtcggggcg agggccatcc cgagtgcggc ggcgacggcg accgccgtca
     8161 acgccctggt gcggcgggtg ggtacgggca tgcgggacct ctcaggggcg cggcggtggc
     8221 tggtcggcga gcgggccccg atcaccggat cgtcaggcgg gcggctcggc ggagggttcg
     8281 ccgcccggcg agcgagttcc tgccgtcccg ttctgcgggt ggccttccgg cgacgggagc
     8341 catgcgctgc gcacggcagg ggttgcctgt actgcttcgt ccgattccgg cgaccgttcc
     8401 catgctcctc gcaccgcgga cctgccagat ctccggcccg ctcccgatgc cggtgcgctt
     8461 gaggatggca aggaccgagc gcagtcgctg gtgcccgcgc gagactcggg tcagacctgg
     8521 cgaaaggggg ccggcacatg tctggactcg ccatcatcac cgaagcctgc atcgacgtga
     8581 aggaccgcgc ctgcgtcgac gtctgcccgg tgcagtgcat ctacgagttc gatgcgacca
     8641 cgaaccagct cttctcggaa gaggaggcgg gtagcggaat cgtcgagaac acccacaccc
     8701 cgaacccaga tgcgatcgcc gtgttcggcg acagcatgct ctacgtcaac ctcgaggaat
     8761 gcacctcctg cactgcctgc tatcagcctg atgtctgtcc ggtcggcgcc atctactccg
     8821 aggaacaagt gcctgacggc tccgccacga ccgccaagta caacgcggaa gatccgcacg
     8881 agggtcacga ccacaccttc ttcgtccaac acagccgcgc tgtcttcgcc gactagcagc
     8941 gacgggaccc acagcgcctt cgggcgcctg ggttcatgct cgaggggaag tggtggttgc
     9001 gtcgacgact tcgctcacgc agcggtggaa cacccgccat cggcgctcgt tgtcatgaac
     9061 gagaccagcg gactcgctga ggaggtcggc cgctcgggcc aggagttccg ccgctgagta
     9121 gagcctgcgg ggcagcaccc gttgcccagt gaggtcggca cgtacggccg ccggggcgaa
     9181 gtcgattcgg tcgatcatgt ccgcagcacg atcgagcagc tctgcgatga gcgtgttcat
     9241 cgacgccgcc aaccagtcgt tcgagatgat tcggtgccgt tctccgagga catcggcgag
     9301 ctcgtcgatg tcgagcaggg cgcgcaccgc ctgccaggaa gcctgcatcg cctgcgtgag
     9361 ccactcgccg atgttgcggc agtcctcggc atgcgtgcgc aggtctcgga tcattgccgt
     9421 gagctcagcg ggacgccctt ccccctccat gaacaagaca ccttcgagct ggagcgcagc
     9481 cggatcgttc agatcctcgg ctccctcgaa ggggctgagc gcagccgagt cgcccacctc
     9541 cacgttgctc cagcgatcgg ccaagctctg cagcgcacgc gcttgtctct gtatgtgaag
     9601 cgcgctctcg cgtggtgctg cggcgtagaa gcgctcgtga tcacgatgga accgcgccag
     9661 gttcatcatc gtgttcagca gtggaccttc ctcgatgccc tccgcagcga cctcgctgtc
     9721 cgacgtcaca cgctgcctcc tcgcgccttc ccctcccagg ttccaggtat cggaggccgt
     9781 ccaccagggc cctccgtccc tactgcgcgt tcttcggggg caggacgagg actccccagg
     9841 ctcggcgggc gcacagatcg ctcggcggat ggcgccacct cctgtgggtc aacccgctgt
     9901 cgaactcagc tgcggctgcg ctcctcgatc tcggcggtcg ctgctacccg cagcgctgac
     9961 cggtcgatgc ccatgcggcg cagtgcccgg gcggtcgggc cgttctcgag gtcggccgcg
    10021 gcgcggagga catgggcgcc gaccagcgca ccaccgcctc ggcgggcgtc ttcgccggcg
    10081 gcctggaaca gctcctgtgc ggagggtgcg ctccgataga tgccgaccgg ttcgtcaggc
    10141 ggtgggagct cggcgctgat gccgtcgtcg tcggcgtgga ccccgatgcg ctcgagatcc
    10201 tcgaccgcct gtgcgtcgag cgccgccccg aagtcggcag ccgagaatcc gaggcgctcc
    10261 agggcacgtg tcgcggtgcc gtcgggcagt tgcagcgccg ccaggacgag gtgctcggcg
    10321 gctggtcgtt ccgtgccgcg cgtccgggcg atctcctccg cccgggtcag gagcgcggcg
    10381 atcgtcttca tggaggcggc gcgtcctcgc agcttgcgga gcatgtcagt ccctccgggg
    10441 tcgggtgtcc gggcgttcga cccggcgggc gaacttcttg tgcgcggctt gtcggctgac
    10501 gccgagggcc gcggcgacgt cggtccacga ccagccctgc cgcacggcga cgttcaccgt
    10561 cgcgagctcg agccggtccg ccaaccgtcg cagcgcgacc accgaggcga acgcggcttc
    10621 cgggtcggag gggtccggta cctcgatcgt gtccggtgtc ccctgcacga gtgccaacct
    10681 acgttgacgg cgggagggtg tcaacccagg ttggcaccct cgatggatcc ggtcagtccc
    10741 gcttcggcac gaggacccac aggacgaggt aggccacctc gccggcgccg accaggccga
    10801 agatgacgaa cagcagccgc acgaaggtcc ggctgacgcc gaagcggtcg gcgatcgccg
    10861 cgcagacgcc ggcgatgacc cgtccctgac ctcggcgcgg acgttccagg gttccggtga
    10921 cagccatgat cggtcgctac ccgggacacc tggacctgac ccgaacttct cgaatcaggt
    10981 gttgcagaac gaacaccagt tcgagtacaa ttcggacatg gccaaggcac cggcgacccc
    11041 gcaggaggtg atcgatctcc tcgaacaggt gcacgacgtg tgcggtcgcc tcgagccggg
    11101 caacgtcccg ctgcacgtcg cgccgcaggt gttcgatgcc ctcgcgtcgg ccgagcgcct
    11161 gatcggcggt gcggtgctgc ggatggcggg ccgctacgag gaggccggcg agtggaagcg
    11221 caacggggcc aagtcggccg aggacgacat cgcccgcaag accggcaccc cgaccgggac
    11281 ggcccgcaag aagctgtcca ccagcaagcg gttgcgcaac cagcagcgca ccgacgaagc
    11341 gatccgcaac ggcgatctgt ccaccgagca ggccaacgag gtgtcctccg gtgccgatgc
    11401 ctcacccgag gacgaagacg acctgctcga caccgcccgg cgccaccgcc tggccgagct
    11461 gcgcaagcgg gccgccgatg cccgggccaa ggccgaccgc gaccgggagg accgtcgccg
    11521 ccgccaccag ggcatgcgca gcgtgcgccg ctggaccgac gaggacggca tgggcaacct
    11581 gtgcctgcgc ctcccacccg aggacatggc cgaggtcgac gccgcgctga agccccgcat
    11641 cgaccgggct ttcgccgatg cccgccaggc cggccgcttc gaaccggtcg agcgctatgc
    11701 cgccgacgtc gtccgcgacc tgctcaccgg cgccaccgac cgcacgccgg cccggcgcaa
    11761 ccaggcggtg cggcccgaca agaaggtcat cgccctcatc gacctggccg cgctcaaccg
    11821 cggttcgatc gagggtgacg agacctgcga gatcgccggc gtcggacccg tgtcggtctc
    11881 cgcggtccgg gccctgctgc acgacgcctt cctggccgtg gtgatcaagg acggcatcga
    11941 catcctccac gtcacccacc tcggccggcg ggtcaccgcc acccaacgca ccgccctcga
    12001 ggcccgcagc ggcggcgcct gcgaggtgtg cggcagccgc tacctcctcg acatcgacca
    12061 caacgagggc tggaccctca cccacgagac ccgcctcgac gacctctcct ggttctgctg
    12121 gcactgccac cgcaccaaga cccgccacga cctgcgagtc gaaggtcccg tcggcgcccg
    12181 ctggttcgtc gaccggaaga ccggcaaacg ctggcgccca ccacccgacg agcctcggga
    12241 gccgccgccc ggcgccggcg gtgcgggaga cagcggggac ccggcgcagg ccgagctctt
    12301 cacgctcgcg gactgacggg acgcaggtcg gacgggagcc gggaagtacc gtcgcggcca
    12361 tggctccggg cacggcgacg accctgcgac ccagcctgaa gcgcaaggcc cccggcaccc
    12421 gtcgcgagcg ggagctgtgg gagctgggcc accaggtggt ggtcgggatc gacgaggtcg
    12481 gccgtggcgc gtgggcgggt ccgctgaccc tcggcgccgt cgtcatcccc aaagacaagc
    12541 gcctcaccaa ggtgcgtgac tccaagatgc tcaccgagcg cgagcgagag gtcatgtacg
    12601 agcgcatcgt cgggtgggcc gacgcctggt cggtcggtca cgcctggccc gaggagtgcg
    12661 acgagctcgg catgtccgag gcccagcgcc tcgccgcccg acgggccatc gagggcctcg
    12721 gcgtcgtgcc cgaccaggtg ctgctcgacg gcaactggga cttcgtgggc agcggcaacg
    12781 tgaccaagat cgtcaagggt gacgccacct gcctgtcgat cgccgcggcc tcgatcgtgg
    12841 ccaaggtgac ccgggaccgc ctgatgcgcg agtgcgccga cgcccacccg tggttcagct
    12901 tcgagaccaa caagggctac ccgtgccccc ggcacaagac cgccctcgcc ggtgtcggcc
    12961 cctccgccat ccatcggcgc agctgggtct tcatggagca cctgccctgg gccggagccg
    13021 tgcgctcggt gccgctccac ctccgaccgg acgactccca ggggaccttg ttcgcatgac
    13081 cgatgcacgc gacgccgagc tggcgcggct gctcagcgag gtccgatccc tgcggctgcg
    13141 gcccgacgac tgggacgagg tggacggcca cctcgccgcc atcgaagccg gcgaccgtgg
    13201 ccgcatcgac gacctgtccc aggcggtgtt cgaggcccgg gtgcgcggtc gcttcagcgg
    13261 cccgcggggg agcgccggtg tggccccgac caagcagacc tcggtcctgc ccatcgtggg
    13321 cctggtctgc ggcggcctgc tcgtcggcgt cggcgccctg ctcggcggcg gtccgatcct
    13381 gtgggccatc gtggccctgg gcgtcttcgt gttcggcatc gccttcgccg gctcacgggt
    13441 cgcccatcgc ccccgtggcg gggccgacga ggaggacacc gtcgagccgg tgccgatccc
    13501 cgatcacgtg gcgctgcggg tcaacgccat cggacgcgcc cacggagccg ggcggtagcc
    13561 gtgcggtgat cagccctcgg gggcgcgcag caccagctgg ggagcgccgt tgccgtcctg
    13621 ggtggggtcg ggaaccacgt cgagggtgag gccgtcgagc tgctcgttga tctggccgtc
    13681 gaccatcagg gtcgtgccgt gctgctcggt gacgacgtcg tcggcctggg gctcggcggc
    13741 gaagtcgatg ccgagaccca cctcgccctc gggtgcgggg gcggggaaca ggcggacgcc
    13801 gtggccgtcc gggatgtcgt tctgctggcg gaccgcctcg agggtggcgg cggcggcggg
    13861 ggtgcactgg agcatggagc tctcctctgc gatcgcggac gacccaccgt agggagggat
    13921 gccgccattc cccacgtggt cagcggcgga gctggctcac caggcgcagg acgtaccaga
    13981 acagcatcat caccgaggcg aacagctgca ccgcggcacc cacgtgggcg tgcgacgggt
    14041 agcgggccat gatctgctgg gtctggtaga ggatggcgcc gccggccagg gcgatcatcg
    14101 ccaccgagaa ccagatgccg aggctcagcc cgaacagcac ggcggcgacg atgagcacca
    14161 gggcagcgat gccgccccac atgagcatcg ggcgcaggaa cgacaggtcc ttgcgggtga
    14221 cgaaggcgac gacggtgagg ccggcgaagc cgaaggcggt gatggcggcc gcggcggcca
    14281 ccgaggtcga gccgttcgag gggtcgttga agaccaggta gaggaacggg gcgaagatca
    14341 gcgcctggcc tgcggccatg ccgaacaggc cggcgtactg gaggccctcg ttctcgatgt
    14401 tgtgggcggc ggaggtggcc atccactgga tgaccatgaa ggcgccgagg atgagcagcc
    14461 acgcgctgct ggcgccgacc aggaagtcgt gcatggcctc ggcgatgccg gtctggaaca
    14521 ggaccgtctc gaaggcgacg aacgcaccga tggcgaggag caggtgctgg tacacccgga
    14581 tcacgaaggc gccacgctcc tcggcgtcga gcgtggcgac cggctgggtc ggggcgaagg
    14641 attccatggc cccattctgc cctgaacggg cgcgaaccgg acggcgcgcg cgatggcgtc
    14701 gggcacaggg ctcgtcggta gcgtcggccc atggaccccg tgcggatgcg tcccgccgtc
    14761 gtgctcgctg ccttcaccct gctcgtctgg accacccgga tccgcaacat ctggaccgac
    14821 gactcgctga gcaccaccgg ccagctcggc cgaaccgcgc tgtcgctgtc gttcacggtg
    14881 ttcgcagccg ccgtcctgtg gctctggtgg cggggtcgtc gggccggctc ggtcgatccc
    14941 atcgccgggc cggtcgtgcg catgttgggc gcgtggacca tcggggtctg ggcggtgcgg
    15001 gtggtgcaga tctccaccgc cggccacgat gccgccttcg tggcggtcca cacggcattg
    15061 gccgtggtct ccacggtgct cgccgtgtgg gcggtccgag aggtgcgacg ggggtcggac
    15121 gccggttctc agcgccttcg aaacgggtag tggtgccggc gatgacagaa ccactgctgc
    15181 tgctggcgtc cgtcggggag gccttcggcg aggtcaacga cgtgttcccc agctccgaag
    15241 cggtctggcg cgtgctcgcc gccgtcgccc tcggtgcgat cgtcggtgtc gagcgggagg
    15301 tgaacgacca gcccgccggg ctgcgcacgc acctcacggt cgcgctgggt gccgcggtgt
    15361 tcggcgtgat ctcgacgctc gggttctccg agttcgtcac cgaccaacgc gacgtcaacg
    15421 tccagttcga tgtcacccgc gtcgcgtccc aggtcgtggt cggcatcggc ttcctcggtg
    15481 ccggcatgat cttccgctcc ggctccaagg tccggaacct caccacggcc gccagcctct
    15541 gggtcaccgc ggcgatcggt ctggccgcgg gcgtcgggga catcggcatc gccatcgtcg
    15601 ccacggccgc gctgctgttc gccctcatgc tgctgcgcat cccccgcgac gccattcgca
    15661 accgcttcac ccgatcgacg gcgcacgtct cgatccgcgt gatgggcacg gccgcggcgg
    15721 agccggtgct cgacgcggtg cgagccatcg acggcatcgc ggtcctggcc gtggggtggg
    15781 gcaagcacga gggcgacacg gtgctcgacc tcaccctcga gggcgacgcc aagatcaagc
    15841 cggcgcgcca cctggccccg atcgtggccc gcgacgacgt catctcggtg aacaccgacg
    15901 tgttcctggg gtccgagatc agcgacgacg cctgacgtct ccggcgatca gatctccacc
    15961 cgggtcccga cctcgaagat gcggtcgggt ggcagtcgga agtagtcggc ggcgctggtc
    16021 gcgttgcggc ccatgacgcc gaagaggcgc tcccgccaga tcgccatgcc cggccgcttc
    16081 gtggcgatca ccgtctcctt gcccaggaag tacgtggcct cgtcggcgtg gaagccctcg
    16141 acgccggcga ggtcccgggc cagatccggg tcctccatga agccgtagtg cacatcgacg
    16201 cgggagacgc cgtcgcccat ctggcgttcc cgcaggcggc tccggccctc gatgcggggg
    16261 acgtcgtcgg tgaccacgga ggcgaccacg acgcgctcgt gcagcacccg gttgtacctg
    16321 acgttggcga ccagcgacgg tggcgtcatc ccggggatgc tgaacaggta caccgccgtg
    16381 ccgggcaccc gggccggcgg gtcgtcggcg aggccggcca ggtaggtgac caggggcgtc
    16441 cggccgagct ggatccggtc gcggacgagg cgtcgaccgg tgctccacgt cgtgagcagg
    16501 gcgaagagca cggcggcggc cacgatcggg aaccaaccgc cggccgggat cttcggcacg
    16561 ttgccggcga agaacgcgag gtcgatgacg agcaggccgc cggcgatcgc gagcacccgg
    16621 ccgagggacc actcccacag ggtccgggcg acgaccgcga acagcacggt cgtgatcacc
    16681 atggtggtcg tcaccgcgat gccgtacgcc gccgcgagcg agctcgacga gcggaacccg
    16741 agcacgaggc cgatgcacgc gaccatgagc acccagttca ccgtcggcac gtagatctga
    16801 ccctgggcgc tccccgacgt gtggacgatc cgcagtcgtg gggcgtaccc gagctgcacc
    16861 gcctgcatgg tgagcgagaa ggcgccggag atcagggcct gcgatgcgat gaccgtcgcc
    16921 gccgtggcca gcacgaccat cgggaccagc gcccagtcgg gggcgagccg gtagaagggg
    16981 ctgtcgatcg cgctgggatc ctcgagcagc agcgccccct gaccgaagta gttgaggacg
    17041 agcccgggca gcgcgaccgc gaaccaggcg atggcgatgg gtcggtgccc gaagtggccc
    17101 atgtcggcgt agagcgcctc cccgccggtg acgacgagga acaccgcgcc gagcacgagg
    17161 atcccggtca gcccgttgtc ggcgaagaag cggacgccgt ggagcggatc cagcgcctcg
    17221 agcacgcgcg gcgccgcgag gatctggcgg gcgccgagga cgccgagcac ggcgaaccac
    17281 agcaccatga tcggcccgaa cacccgaccg accacgtgcg tgccgtgccg ctgcaccgag
    17341 aagatggcga tcaggatcgc gatggtgatg ggcaggacga ggtcggcgag ggcgggagcc
    17401 gcgatggtcg tgccctccac ggcgctgagg accgagatcg ccggcgtgat catcccgtcg
    17461 ccgtagagca gggcggtgcc gaagaggccg agcagcacca gcacccaccg cccgccccgc
    17521 tgctgcgcgg gcgtcacgag cgaggtgagc gtgaggatgc cgccttcccc gtggctgtcg
    17581 gctcgcatga cgagggcgag gtacttgacg gagatgacga tgacgaggga ccagaacacg
    17641 agcgagagga cgccgagcac gttgctctcg aggacgtcca gctcgtggcc gtggccctcg
    17701 aaggcctccc ggaacgcgta cagcgggctg gtgccgatgt cgccgaacac caccccgagg
    17761 gcgccgaggg ccagcatccc ggtgcggggg cggcctgggg tcggttcggc tgccacgggg
    17821 ggacgcccct caggggcggg agagctcgat gtccagggcg aaggtgaggc agcggcagcc
    17881 gccgccggcc ttgaggaact cgtccaccgg gcacaccacg acgtggaagc cccaggcctc
    17941 gagctggcgg ccgacccgcg gcgggcacgt cggcatgtgg atcacgtcgc cgacgacgac
    18001 ggagttggcg cagaacgcga gcgcctcgtc gtcggtgagc cacagcggct cggggaccag
    18061 ggactccacg agccgccggc cgtaggtgtc ccagccgagc ggggcgcaca tggcacggcg
    18121 gtcgtccagg gggcagaagg tcaggtcgag gtggtacagc cgctcgtcga cgagctccag
    18181 ggtgcgcacc ggcacgtcga gcaggccggt gaggtggggg gcggcagcca ggtcggagcg
    18241 gggccggtag ccggagacca gcacgccgcc gaaggggagg gcgtcgccgg caccttcgtg
    18301 caccacctcc tcgggcaggc gggtgacctc ccagccggcg tcgtcgaacc actcggcgaa
    18361 cacctcggtc tccggctccc gctcgggatg gcggaagtgc gacgggatga accgcttcgc
    18421 cgtggcgtcg accaggcctg cgttggcggt gaagacgagg tcggggacgc cctcgggctg
    18481 ctcgatggtc tcgacctcgg ccccggcctc gacgagggcg tcgtgcaggc cgtgccactg
    18541 ctcgagcgcg gcgtcgacgt cgacggcgac gccgcgctcc atccacgggt tgatctcgta
    18601 gaggaccccg aagtggcggg gctggcacag caggaagcga cgcccccatc cgagctgggt
    18661 cacgagcaga gctcgccggt gagctggcgg acgaacacgc cgatgtcggt gacgatgccc
    18721 acggcctggt gcgagccccg gtcggcgagc ttggtcacga cggcctggtt gatgtcgacg
    18781 cagaacgtct ccactgatgc cgggaggatg ttgccggtgg cgatggcgtg cagcgtggtg
    18841 gcgagcatga gcgcgacctg caccccgggg acctggtccc gcatcgcgtc ggcggcctcg
    18901 gcgacgtcgg tcatggtgtc gggcagcggg ccgtcgtcgc ggacggagcc ggcgagcacg
    18961 aagggcaccc cggcccgcac gcaggcgtgc atgacgccgc cggtgacgta gccggcgtcg
    19021 accgcggcgg cgatggagcc gtgccggcgg atctcgttga tgacccgcac gtggttggcg
    19081 tggccgccct cgaccgggac cccgtcggcg accccgaccc cgagcgaggt gccgagcacg
    19141 ttggactcga ggtcgtgggt ggcgaacccg ttgccggtga acaggcagct gatccagccg
    19201 tgctcgacga gcttggccag atcggggccg gcgccggtgt ggatggcggc gggaccggcg
    19261 acgagcagca cccggccgcc ctcctcgcga gcggcgcgga tccgctcggc gacctggcgg
    19321 atcatcaggc ccttgggctt ctccgaggag acctcggagt tcatgaactc gaacgtgccg
    19381 gccaccgccc ggcgctcggg cggtcggacc cggaccccgt cgcggcccac gacgatgcgg
    19441 tcgccggccc gcacccggtg catggggatg gtgcggaccc ggagctgccc gtcgggctcg
    19501 tcgaccacga tgccgcagtc catctccggg ttctcgaccg tgtgccaggt gccgtcgatg
    19561 agcacgtcgg tgtcgaggtt ggtggtggag tggaagccgg tcggcaggac gccgtcctgc
    19621 tcggccgcct cgagcacggc ctcggactgg gcgatgcggt tggccccgtg gacctgcagg
    19681 tcgtcgagga gggcgtcgag cgtggcctgg tcggcggcgt ggaccgcgat cgacgcccgg
    19741 gaggagtcgg tgttggtctt gccgatcacg acgtcggtca gctcgtagtc cgcgccggct
    19801 tcgaggatcg cgtcgaggat cttggccagg atcagcgagt cgacgatgtg gccttgcacc
    19861 tggatggtct cggtcgccat cggcccatct tctcgccccg gcgacctcgg cgctcgcctt
    19921 tcccgccgac ggagggcgag cggtaccatc cggccccgca tgggacagag cgtgaacgtc
    19981 gtcgcgaagc actcgtcgaa ccccgagatc ctgcggttcg agatcaatcg cagcctcacc
    20041 gggatgggcc acgagcgcta ccgctcgctg gacgacctcg tcctcgaccg cccggtcgac
    20101 cgcctcgccc gccggctcct cgagcacggg ggcgtcgacg gcgtgcacgt gaactcctcg
    20161 gtgatcaccg tggacctcgg cggcgggtac gtcggcgacg gcctgcagca gatcatcgag
    20221 gacctgttcc gcttctaccc cgacgccccc gacggcaacg atgcgcccga ggccgacgag
    20281 gcgtcgaccg aggtcgcggc cgaggacgcc ccggtggcgc aggagcccct cacgggcgac
    20341 gagaccgagc tgcgcgaggc acccgaggcg gcggccgcca ccggcgacgg cgagctcgac
    20401 acgccggccg aagccccggc cgaggcaccc gaccccgacc ggcccgacga cgaaggcccc
    20461 ccggccgact gatcatggtc gacgctgccg attggtccct gcggttccat cggcggctgc
    20521 tcgccggcga ccgcttcggg gccacgagcg tcatcgaggc gtccctcacc gccggcaccg
    20581 atccggtcgg ggtctacctc gacgtggtcg caccggcgat gcgggagatc ggcgagggtt
    20641 gggccgccgg cacgatcggg gtggccgagg agcaccgggc ggcggtcatc gtcggtcagg
    20701 tgctcgccgt gctggacagc cgcttcagcc ggcgcagcgg ccggcgcggc ctggtggtgg
    20761 ccggggcggt ggagggggag cgccactcgc tcccgctccg gatc
//
LOCUS       DATISU010000094         4643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_284035, whole genome shotgun sequence.
ACCESSION   DATISU010000094 DATISU010000000
VERSION     DATISU010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284035"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VLR27_04050"
     CDS             complement(<1..201)
                     /locus_tag="VLR27_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02646.1"
                     /translation="MPGRNDDERVPPLRRLWHYGAPYRRRTGWATTHSVLNKLFDLAP
                     PLLIGLAVDVVANDGDATLDRLG"
     gene            374..2641
                     /locus_tag="VLR27_04055"
     CDS             374..2641
                     /locus_tag="VLR27_04055"
                     /inference="COORDINATES: protein motif:HMM:NF007088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HSP02647.1"
                     /translation="MPDAVPQVLRRLADLRSWASTPARRRIAYAVALALFVGSTVVAW
                     QALPARGELDWGLVALAALLVLPGAVINAEEYRVSGRIVGQRIALPAALRVSIIAAAF
                     NLLPIPGSVLVRTRALAKGGSSTARAVTSTLAVGLAYLAVALVIVAGVQLASAPVQAA
                     VLAVIGLAMLAGTGVLVRRLADGPAGGLLRRLVLVEALSVVVKALRLLLTIQALGFEP
                     SIAQATSLSMATVAASAIGIFPGGLGIRELLSGLIAPAVDLPASVGLVGTSMDRLIGL
                     ATLSVLAAIVLFATRERLPRPDGASHPSVVADIDAVFKAYDIRGITPDQLDAELVRSI
                     GAAFARFVRTQPEPHDRVLVGRDMRPSGVELVAAFCEGVTSQGMDVTRIGLASTDMLY
                     FAAGSLDAPGAMFTASHNPAQYNGIKMTLAGAKPIGSDTGMDEIKRMILEDDLSPQGE
                     PGTIDDVELLDAFADHVRSFIDTDALVPVKVVADTANGMGGLVVPAVFEGLPVDLEVM
                     YGELDGTFPNHPADPIDVENLRDLQARVLEVGADVGLAFDGDADRVFLVDDLGQPVSG
                     SITTAIVAKGILEKEPGATILHNLICSKTVPEVVIENGGTPIRTRVGHSFIKATMAET
                     GAAFGGEHSAHYYFRENWRADSGCIASLVVLEQLSRTGQKLSELRKPFERYAASGEIN
                     TTVDDPAAVIDRVAAAYADHDQDRLDGLTVDLGDWWFNLRPSNTEPLLRLNLEAATRE
                     QCDAKVEEVRSHFVG"
     gene            2652..2900
                     /locus_tag="VLR27_04060"
     CDS             2652..2900
                     /locus_tag="VLR27_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HSP02648.1"
                     /translation="MALDQLLLDILACPEDKGPLYYFEDEDLLLNPRLKRTYAIRDDI
                     PIMLIDEATTVDDAEVERLLAKAEAEGITPTFDPDDGA"
     gene            2904..3938
                     /locus_tag="VLR27_04065"
     CDS             2904..3938
                     /locus_tag="VLR27_04065"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HSP02649.1"
                     /translation="MSPLLPDGPIDSLGVLDAVLATPESVAAAASAPVDNPPFAGVHG
                     MVLVASGTDALVAEAAQALVSPWSPVPVFVHTGHGLPDFVSDEWRCVFVSRAPTPEST
                     SALRAAIDHQAVLTAVGSGDVVEAIAERDGGVLRCTDDVPAPRFAFASTLVSTLRLLD
                     GLSALATPLGGPGLDETIEAAVHQLGRRRDQMDRDDSEAATLARRIGRTMVLTLGLGP
                     LGAVAARRWKQQVNDNVKAPAFDTALPQALHHELSGWGQHGDMTRQVFSLVELRHDHE
                     HTEDAARLPAVEAAFEEVVHARHVVQAEGQGPLAQLLDLAFVGDLVSWHLAQENEIDP
                     GPRGVDTLDA"
     gene            3941..4210
                     /locus_tag="VLR27_04070"
     CDS             3941..4210
                     /locus_tag="VLR27_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3986..3988,aa:Sec)
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HSP02650.1"
                     /translation="MLDPRHRISIAFCVPUDYVPRAVGLTDELLRGWAPIIETLELIP
                     AGKGRFEVTLDGELIFSKAELGRHAEPGEVVALARERMGPEIERG"
     gene            4324..>4643
                     /locus_tag="VLR27_04075"
     CDS             4324..>4643
                     /locus_tag="VLR27_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HSP02651.1"
                     /translation="MTTPKTDGRDFKVADLSLAPWGRKEIQLAEVEMPGLMALRAEYA
                     DAQPLAGARIMGSLHMTIQTAVLIETLTALGAEVRWVSCNIFSTQDHAAAAVAVGPDG
                     TPDKP"
BASE COUNT          631 a         1786 c         1580 g          646 t
ORIGIN      
        1 cccgaggcgg tcgagggtgg cgtccccgtc gttggccacc acgtccacgg ccaggccgat
       61 cagcagggga ggggcgaggt cgaacagctt gttgagcacc gagtgggtcg tggcccaccc
      121 ggtgcggcgg cggtacggcg ccccgtagtg ccagaggcgc cgcaggggcg gcacccgttc
      181 gtcgtcgttc cgtcccggca tccgacgagt ctggccccac ccgatccgtt ccccgcgcgc
      241 gaatcgacca tggagggtcg atcggcgcgt caggaaccgg aagggggccg ttcgttgcgc
      301 gcgattcgac cgcctgcggt cgatcggcgc gcagggaacc gggcgggtgg gtgccggtgc
      361 ctacgatccg gccgtgcccg acgccgtccc ccaggtgctc cggcggttgg cggacctgcg
      421 gtcgtgggcg tcgacgcccg cccggcgccg catcgcctac gccgtcgccc tggccctgtt
      481 cgtcggctcg accgtggtcg cgtggcaggc ccttcccgcc cggggcgagc tcgactgggg
      541 gctcgtggcc ctggccgccc tgctcgtcct gcccggcgcc gtgatcaacg ccgaggagta
      601 ccgggtgtcg ggccgcatcg tcgggcagcg gatcgccctg ccggccgcgc tccgggtgtc
      661 gatcatcgcc gcagcgttca acctgctgcc gatccccggt tcggtcctgg tccgcacacg
      721 ggcgctcgcc aagggcgggt cgtccacggc gcgggcggtg acgtcgaccc tcgccgtcgg
      781 cctggcctac ctcgcggtcg ccctcgtgat cgtggccggg gtgcagctcg cgtcagcgcc
      841 cgtgcaggcg gccgtccttg ccgtcatcgg cctggcgatg ctcgccggga ccggcgtgct
      901 ggtccggcgc ctggccgacg gaccggccgg cgggctgctg cgccgactcg tcctggtcga
      961 ggccctgtcg gtggtcgtca aggccctccg cctcctcctg accatccagg ccctggggtt
     1021 cgagccgagc atcgcccagg cgacgtcgct gtcgatggcc acggtggcgg cgtcggccat
     1081 cggcatcttc cccggcgggc tcggcatccg cgagctgctc tccggcctca tcgcccccgc
     1141 cgtcgacctg ccggcatcgg tgggcctggt cggcacctcg atggaccgcc tcatcggcct
     1201 ggcgacgctc tcggtgctgg ccgcgatcgt cctgttcgcc acccgggagc gcttgccaag
     1261 gcccgacggc gccagccacc cttccgtcgt ggccgacatc gatgctgtct tcaaggcgta
     1321 cgacatccgg ggcatcaccc cggaccagct cgacgccgag ctcgtccgct ccatcggcgc
     1381 ggcgttcgcc cggttcgtgc ggacccagcc cgagccccac gaccgcgtgc tggtcggtcg
     1441 ggacatgcgc ccgtcgggcg tggagctggt cgccgcgttc tgcgagggcg tgaccagcca
     1501 gggcatggac gtcacccgca tcgggctggc ctccaccgac atgctctact tcgccgccgg
     1561 cagcctcgac gcccccggcg ccatgttcac ggccagccac aacccggccc agtacaacgg
     1621 catcaagatg accctcgccg gcgccaagcc catcggctcc gacacgggca tggacgagat
     1681 caagcggatg atcctcgagg acgacctgtc cccgcagggc gagccgggca ccatcgacga
     1741 cgtcgagctg ctcgacgcgt tcgccgacca cgtgcgcagc ttcatcgaca ccgatgcgct
     1801 ggtcccggtc aaggtcgtcg ccgacacggc caacggcatg ggcggcctgg tcgtgccggc
     1861 cgtgttcgag ggcctccccg tcgacctcga ggtcatgtac ggcgagctcg acggcacctt
     1921 cccgaaccac cccgccgacc cgatcgacgt cgagaacctc cgcgacctgc aggcccgggt
     1981 gctcgaggtc ggtgccgacg tcggcctggc cttcgacggc gacgccgacc gggtgttcct
     2041 cgtcgacgac ctgggccagc cggtgtcggg ctccatcacc accgcgatcg tcgccaaggg
     2101 catcctcgag aaggagcccg gcgccacgat cctgcacaac ctgatctgct ccaagacggt
     2161 gcccgaggtc gtcatcgaga acggcggcac gccgatccgc acccgggtgg gccacagctt
     2221 catcaaggcg acgatggccg agaccggggc cgcgttcggc ggcgagcact cggcgcacta
     2281 ctacttccgg gagaactggc gggccgactc cggctgcatc gcctcgctgg tcgtcctgga
     2341 gcagctgagc cgaaccggcc agaagctgtc cgagctgcgc aagcccttcg agcgctacgc
     2401 cgcgtccggc gagatcaaca ccaccgtcga cgatcccgcc gccgtcatcg accgggtcgc
     2461 cgccgcctac gccgaccacg accaggaccg cctcgacggc ctcaccgtcg acctcggcga
     2521 ctggtggttc aacctgcgcc cctccaacac cgagccgctg ctgcgcctga acctggaggc
     2581 ggccacccgg gagcagtgcg acgccaaggt ggaggaagtc cgctcgcact tcgtggggta
     2641 gaacgtcccc catggcactc gaccagctgc tgctcgacat cctcgcctgc cccgaggaca
     2701 agggcccgct ctactacttc gaggacgagg acctgctcct caacccgcgg ctcaagcgca
     2761 cctacgcgat ccgcgacgac atcccgatca tgctgatcga cgaggcgacc accgtcgacg
     2821 acgccgaggt cgagcgcctg ctcgccaagg ccgaggccga gggcatcacg cccaccttcg
     2881 atcccgacga cggcgcctga gcggtgtccc cactgctgcc cgacggaccg atcgacagcc
     2941 tcggcgtcct cgacgccgtc ctggccaccc ccgagtcggt ggccgcggcg gccagcgcgc
     3001 cggtggacaa cccgcccttc gccggggtgc acggcatggt gctcgtcgcc tccgggaccg
     3061 acgcgctggt ggccgaggcc gcgcaggcgc tcgtctcgcc ctggtcgccg gtgccggtct
     3121 tcgtccacac cgggcacggc ctgccggact tcgtctccga cgagtggcgc tgcgtgttcg
     3181 tctcgcgggc accgacgccg gagtccacct cggccctgcg ggccgccatc gaccaccaag
     3241 cggtgctcac cgccgtcggc agcggcgacg tcgtggaggc catcgccgag cgcgacggcg
     3301 gcgtgctgcg ctgcaccgac gacgtgcccg cgccccggtt cgcgttcgcc tccaccctgg
     3361 tcagcaccct gcgcctcctc gacggcctca gcgccctggc caccccgctc ggcggtcccg
     3421 gcctggacga gaccatcgag gccgcggtgc accagctcgg ccgccggcgc gaccagatgg
     3481 atcgcgacga cagcgaggcc gccacgctcg cccgccgcat cgggcgcacg atggtcctga
     3541 cgctgggcct cggccccctc ggtgccgtcg ccgcccggcg ctggaagcag caggtcaacg
     3601 acaacgtgaa ggccccggcc ttcgacaccg ccctcccgca ggcgctccac cacgagctgt
     3661 ccggctgggg ccagcacggc gacatgaccc gccaggtgtt ctccctcgtg gagctgcgcc
     3721 acgaccacga gcacaccgag gacgcagccc ggctccccgc cgtcgaggcc gccttcgagg
     3781 aggtcgtgca cgcgcgccac gtggtgcagg ccgaggggca ggggccgctg gcccagctgc
     3841 tcgacctggc cttcgtcggc gacctggtca gctggcacct agcccaggag aacgagatcg
     3901 atccaggtcc caggggcgtc gataccctcg acgcatgacc atgttggacc cgcgccaccg
     3961 gatctccatc gcgttctgcg tcccgtgaga ctacgtcccg agagccgtcg gtctgacgga
     4021 cgagctgctg cggggctggg ccccgatcat cgagacgctg gagctgatcc cggcgggcaa
     4081 gggccgcttc gaggtcaccc tcgacggtga gctgatcttc tccaaggccg agctcggccg
     4141 ccacgccgag cccggcgagg tcgtcgccct cgcgcgggag cggatgggtc cggagatcga
     4201 gcgggggtag acggggagcc ggccctctag gctgcgcacc cggccggctg tcacgagagg
     4261 cgccagttgc gtccagccgc cccttcccga acaccgaacg caacgacgag gagctggcgc
     4321 gccatgacga cgccgaagac cgacggccgc gacttcaagg tcgcagacct gtccctggcc
     4381 ccgtggggcc gcaaggagat ccagctggcc gaggtggaga tgcccggcct gatggcgctg
     4441 cgggccgagt acgccgacgc ccagccgctg gcgggcgccc ggatcatggg cagcctgcac
     4501 atgacgatcc agaccgccgt gctgatcgag acgctcaccg ccctcggcgc cgaggtgcgc
     4561 tgggtcagct gcaacatctt ctccacccag gaccacgccg ccgccgccgt ggccgtcggc
     4621 cccgacggca cgccggacaa gcc
//
LOCUS       DATISU010000095        14658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_610174, whole genome shotgun sequence.
ACCESSION   DATISU010000095 DATISU010000000
VERSION     DATISU010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 14658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14658
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610174"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /gene="secD"
                     /locus_tag="VLR27_04080"
     CDS             <1..1336
                     /gene="secD"
                     /locus_tag="VLR27_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HSP02652.1"
                     /translation="AGLDGLEQPAEEPGEEPAEEESGDEESEDEPTSTTAGDDTSTTI
                     DAPDEQGYGLAPGELAAPLQTDDTSTTTTVAEESAEEEPAEETPAETPPPTGLEELEL
                     TPAEDDVAEEPVTLAQYDDDGNEIARYRLGPAEATGEILSGASAQIQGVGQWAVGVQI
                     RGNRIDEFNAIAGTCFNGAPTCPTRQLAIVLDGRVVSAPSINAPSFSPDDISISGDFT
                     EGEAKDLALVLRYGSLPVELEAQQTQSISASIGQDALDAGVTAGLIGLGLVTIYMLVY
                     YRLLGLVAMLSLSVSAALLWTMIAYLGENQGLALTLAGVTGIILSIGVAVDSNVVFYE
                     HLKEEVASGRSLRAAVDGSFATAFSTIVKADVASLIGAAILYWLTVGPVRGFALFLGL
                     STLLDLVASYFFMRPLTVLLVKSERLQHRPRLFGMPTPKVHRAPTIDEGAPA"
     gene            1333..2406
                     /gene="secF"
                     /locus_tag="VLR27_04085"
     CDS             1333..2406
                     /gene="secF"
                     /locus_tag="VLR27_04085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HSP02653.1"
                     /translation="MSVRSKLASFDNDIDFPRLWRIGAVISLVLLLLSGGALLFRGLN
                     LGIDFEGGTAWEVDAPDASVSEARDVLTPLGLGNAKIQFVGTDNLRVQAEPGDDARLD
                     EVTTELAELAGVDRSEVAISTVGPTWGDEILSKAQRALVVFLVIIAAYMAIRLEWKMA
                     VGALAALVHDIIITVGFYALFQLEVTPATVIAFLTIMGYSLYDTIVVFDKAQANAERP
                     ALAQKMNYTDLMSLSLNQVLMRSLNTTITSLLPVLSMLLVGSFLLGAVTLQEFAFALA
                     VGLAIGAYSSVFVATPAVAWLKEREPHQQNLRQRLDAQGGRRSGEPVTAGATTPESRP
                     VGAAPQLSGRAIPPRPRKKGKRR"
     gene            2505..2945
                     /locus_tag="VLR27_04090"
     CDS             2505..2945
                     /locus_tag="VLR27_04090"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HSP02654.1"
                     /translation="MGPWPRFGARIIDGLVLFIPNAIIAAVLTDDTATSFGGVGTGEN
                     FAYLLVSTLLGFAYAVWFEASRGQTVGKMALGYKVVGPDGRVPSTDVAMRRNAWLLLG
                     LVPIVGGVLSFVAAVAIGVTIANDPFKRGTHDNFAGGSAVVRAR"
     gene            2961..5210
                     /locus_tag="VLR27_04095"
     CDS             2961..5210
                     /locus_tag="VLR27_04095"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HSP02655.1"
                     /translation="MPSTVDRVLPWRRSSPPPAEEVAPLLTAFRARHGRTARTDLITR
                     AYLSSSAAHHGQLRQTGEAYIHHPIAVAKIVADLGLDDVTIAAALLHDAVEDTAMSLA
                     DVEEEFGAEVAAIVDGVTKLDRIHFDTKEQQQAATVRKMIVAMSKDLRVLIIKLADRL
                     HNMRTIAGMAHFKQERTARETLDIYAPLAHRLGMQEVRQQLEDLSFAALHPKRFAEID
                     HMVSTRTPERDLYLHQVIHQVEERLEELRLTAEVTGRPKHMWSIYEKMVVKGRDFDDI
                     FDLVAIRVVVDSVKDCYAALGSIHATWKPVPGRFKDYIAMPKFNLYQSLHTTVVGPQG
                     KPLEVQIRTREMHARAERGVAAHWQYKSNDKPHERSHDDDLAWLSRIVDWQEESTDPD
                     EFMKNLKVDLEQDEVFAFTPKGRVITLPKGATPIDFAYTVHTEVGHTCIGAKVNGRLV
                     PLHSELSSGDTVEVVTSKVEGAGPSRDWLQVVVSPRARNKIRHWFSRERREDAIETGR
                     EDLVKALRRENVPIKTEPVTAALQDIANQANYADLDSLYAAIGEHHLSAKSVAGRVVK
                     ALQDTDVVEPEQLATNLVHDRRSRSRARKSKTGVHVEGLDDVMVRLSRCCTPVPGDEI
                     VGFITRGRGVSVHRADCANAVSLGTGQRDRIIDVEWDGDSAGVYVASIEVKALDRSRL
                     LFDVTSALSDHHVNILASSTTTGSDRVSTMRFEFELGDPAHLDSAISSIRAIDSVYDA
                     YRVVPGKGG"
     gene            complement(5235..5807)
                     /locus_tag="VLR27_04100"
     CDS             complement(5235..5807)
                     /locus_tag="VLR27_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02656.1"
                     /translation="MIDGLNRFLLLVLGLLLAAAGALGLAAGEGTFDDLDSPATVYGD
                     VRAEVVADPDLWYAVALGVSGLVLVLALVWLFRQLASRPSGPHLSTTVVAAGRRGRTT
                     LEPVAVAKAMAHDLETVDGVRSAAVRIRSLGQEPTMRVRLTVDRDADPDRLLEDVEPA
                     LQRGAQSLDAQDVEARIRLDFAGRDESRVV"
     gene            complement(5809..6357)
                     /locus_tag="VLR27_04105"
     CDS             complement(5809..6357)
                     /locus_tag="VLR27_04105"
                     /inference="COORDINATES: protein motif:HMM:NF039399.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6286 domain-containing protein"
                     /protein_id="HSP02657.1"
                     /translation="MTLLRRFLAFVVALALAATAVLVIVESVGIRIDESPVLLPIDDW
                     EQRLTAGDWTTWGADAWTIASAIALGVGLLLVVLQLIPHRTTTLDRRRADGEREVRFG
                     RSGLDERLRDLVVDQEGVLGGKAKVSKRKTKVTARIPDGADPDPTEQSVRTVVRDELD
                     RLRLAKKQRVRVDAAHTDDRVI"
     gene            complement(6354..6716)
                     /locus_tag="VLR27_04110"
     CDS             complement(6354..6716)
                     /locus_tag="VLR27_04110"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HSP02658.1"
                     /translation="MPADADADPRPPAPVDVARIVADAARAHDDVVDLDAGLAGEFAT
                     YGLGGRVPGVRVERDRDGRTKVQVRLVVRFGRALPDIGDEVRSRVTARLDAAEPEVHV
                     HIADIVSEAAELDHPGAP"
     gene            complement(6720..7196)
                     /locus_tag="VLR27_04115"
     CDS             complement(6720..7196)
                     /locus_tag="VLR27_04115"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HSP02659.1"
                     /translation="MTDTSTSTQTPSTSTNRPSPSGGGSGSLARLETDKGVTSISEDV
                     VGKIAAIAAREVEGVESLGGSISGALSSVVGRIRGDEHKTAGVGVEVGTKQAAVDLSM
                     TVRYPASIMDVTGAVRQNVIDRIENMTGLEVVEVNIAVNDLAFPGGDEDQNTGRVE"
     gene            complement(7241..7423)
                     /locus_tag="VLR27_04120"
     CDS             complement(7241..7423)
                     /locus_tag="VLR27_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02660.1"
                     /translation="MQSNLRFWIIVGLVVGGVWAFTGLDGALLTLGLGAVGLLLGIVI
                     EQGVNVRDVLRQDRDT"
     gene            complement(7441..7755)
                     /locus_tag="VLR27_04125"
     CDS             complement(7441..7755)
                     /locus_tag="VLR27_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HSP02661.1"
                     /translation="MGSPYEAAVMTPHRARAILAVPTDASPDEIERAFRRGMRVSHPD
                     HGGDTGRAQLLLDARRCLHGETTRSTHAARRVVVVPAPKRRDRLSALLTRLLPRRPTR
                     VR"
     gene            7916..9199
                     /gene="hisS"
                     /locus_tag="VLR27_04130"
     CDS             7916..9199
                     /gene="hisS"
                     /locus_tag="VLR27_04130"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HSP02662.1"
                     /translation="MSTPAFSAPIGTRDVLAPESARWIALLATFGEIAQRYGYGMVHT
                     PMFEELGVFQRMGATTDVVTKEMYDFEDKGGRRIALRPEGTASVVRAFVEHRPLVPWK
                     AWYVTPAFRYEKPQKGRYRQHHQVGVEVLGVDDPDVDVEVIALAERYLQSLGASRYLL
                     IINSMGDAEDRANYMEILRGYLGDVREQLAEEDREKVERNPMRVLDSKRRETMQATAD
                     APRISDVLSAESVERFERVQAGLRALDIEFAIDPRLVRGLDYYTHTLFEFQSTALDAA
                     QSTIIGGGRYNGLVEALGGPETPGIGFGSGIERVLLHLDAEDAFPTPDTGVDVFVIDT
                     TGGHHARDIAEELRDHGLSADRAWDDRSMKAQMKAADRSGARIAVIVGADEATAGHAT
                     IRDLRGDRGQRSVARPDLVDEVAALLAPDPQVETP"
     gene            9196..10971
                     /gene="aspS"
                     /locus_tag="VLR27_04135"
     CDS             9196..10971
                     /gene="aspS"
                     /locus_tag="VLR27_04135"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HSP02663.1"
                     /translation="MTEILSTGPAVGLRTHWCGELRASDIGATVSLCGWVENRREHGE
                     QLAFIDLRDRTGITQCVVAKAHDLRGEYVLRITGTVQARFEGATNDKLDTGEIELQDC
                     TVEVLSTSEPAPFPIHERTEVDETIRLRHRYLDLRRARLQKNLQVRTIVNAAIRRTFI
                     DNGFVEVETPMLIASTPEGARDFVVPSRLHPGSFYALPQSPQLYKQLCMVGGVDRYFQ
                     IARCLRDEDLRADRQFEFTQLDMECSFVGQEEVLDFVTQSVRAAVADITGDDIGDVPR
                     MTWEEAMERYGSDKPDTRFGMELVELTELFAETEANVFKAPCVKGIRLEGGAELTRKQ
                     IDGLVDQAKLWGAKGLAWMRVVSGEREMPALDGGVAKFLSETESVATITALDAAPGDM
                     VFLVADERPVARHVLGLLRVELGKPPMDEGGLEFLWVTDFPMFDDELGPDGRPQAMHH
                     PFTMPNAEDLEAGLIESDPTKVRSVAYDLVCNGWELGSGSVRIHRSDVQQRVFDALGI
                     AQEEAQEKFGFLLDALRFGPPPHAGFAYGIDRLVALLAGETNIREVIAFPKTQKGADP
                     LTDAPSELEPRVLEELGLRVLPRLD"
     gene            complement(10972..11709)
                     /locus_tag="VLR27_04140"
     CDS             complement(10972..11709)
                     /locus_tag="VLR27_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02664.1"
                     /translation="MTEPLDPLAADEVLRRAAEISAQDRPAPAHSGYDRRALTEAASE
                     VGIERGAVELALAEHDAGILAHTDARGGLLGPSRVLETRVVPRTVAESRGHVTHWLRS
                     QLLHRDERQGSAEVWRPRDDLGAKLRRKIDARTARRVRLGDVDAVEVSVVDCGEGRSL
                     VRLEVVFDDMRRGLRTGVVVIPTAVTPVLGVAAAVLTDPWVAFASLPLAGALGAVGTV
                     AGRKTLADQRERARRALCSFLDGLEDA"
     gene            11756..12805
                     /locus_tag="VLR27_04145"
     CDS             11756..12805
                     /locus_tag="VLR27_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HSP02665.1"
                     /translation="MQVHHLRSVDGEIVYDMDPEIVTPAAGGTRMVDDVTVDELALLA
                     RAMTYKLAVFEGRMGGAKAGIRPRLLDSRAETITRYCDEIRPLVESGRFFTGADLGTT
                     TADFADLGDDTPAVFREVIDGVGFEELVTGRGVVAAAAAWLGTLEDRTVAIEGFGKVG
                     HGVAREVLRRGGRVVAVSTLRGAVAKAGGFDVDELVQARAEHGESFVHHLGLDVHLPR
                     ELHELRVDVLVPGARVGVYDAALAARVQAEVIAPAANVPYTVGGLAELRARRVAALPD
                     FVCNGGAVLAYQTSGALTPAEVLARVDREIGERIEAARLAKMDPIRHAAVLAETFLST
                     WVPADQRPDGPAVAP"
     gene            12802..14148
                     /locus_tag="VLR27_04150"
     CDS             12802..14148
                     /locus_tag="VLR27_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HSP02666.1"
                     /translation="MSDDLFAAAASSRLESRQPLAARLRPRSLDDIVGQEHLLGPGKP
                     LRSLVERDRLSSALLWGPPGTGKTTLARAVAAHTEKVFVQLSAVTATVKDVRETIAQA
                     RHRLGEQGRGTILFLDEIHRFNKAQQDALLPAVEEGLVVLVGATTENPYFEVNAPLRS
                     RSTLFRLEPLTTDAVRTLLGNGLEAEGATATDEAIEHLAERANGDGRQALTALEVAVA
                     LATARAEAGEGSGGNEGNEGSGRPEVTLADAEAALGTNALRYGVDDHYDVVSAFIKSI
                     RGSDPDAGLYWLARMVEAGEDARFIARRLVILASEDIGMADPTSLLVANAAAHAVEFV
                     GLPEAGHNLAQAVVHLSSAPKSNRVAKAWWAAQSDVRDQPAGEVPAHLRDAHYQGAGA
                     IGHGRGYRYPHDDPRGWVEQEHRPEGVAGRTYYEPSEHGAEAEVARRQRERTPRGG"
     gene            14173..>14658
                     /locus_tag="VLR27_04155"
     CDS             14173..>14658
                     /locus_tag="VLR27_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HSP02667.1"
                     /translation="MRADELRRAWTQFFVDRGHTAVPSAGLIPHHPSAPMFVNSGMMP
                     FVPYFLGEEAPPFDPARAVSVQRCVRAGGKHNDLDAIGRSRRHLSFFEMLGNFSFGDY
                     FKEDAIPWAWEFVTEVLGLDGDRIWITVHTSDDEAEDIWADAVGFPRERIQRLDKDNF
                     WE"
BASE COUNT         2126 a         5470 c         5066 g         1996 t
ORIGIN      
        1 cgccggcctc gacgggctcg agcagcccgc cgaggagccg ggggaggagc ccgccgagga
       61 ggagtcgggc gacgaggagt cggaggacga gcccacctcc accacggccg gcgacgacac
      121 ctcgaccacc atcgacgccc ctgacgagca gggctacggc ctcgcccccg gcgagctcgc
      181 cgccccgctc cagaccgacg acacgtccac cacgacgacc gtggccgagg agtcggcgga
      241 ggaggagccg gccgaggaga ccccggccga gacgccgccg cccaccggcc tcgaggagct
      301 cgagctcacg ccggccgagg acgacgtcgc cgaggagccg gtgacgctgg cccagtacga
      361 cgacgacggc aacgagatcg cccggtaccg cctcggccca gcggaagcca ccggcgagat
      421 cctcagcggc gccagcgccc agatccaggg cgtgggccag tgggccgtcg gggtgcagat
      481 ccgcgggaac cgcatcgacg agttcaacgc catcgccggc acctgcttca acggtgcccc
      541 gacctgcccc acccgccagc tcgccatcgt gctcgatggc cgggttgtgt ccgccccctc
      601 gatcaacgcc ccctcgttca gccccgacga catctccatc agcggcgact tcaccgaagg
      661 cgaggccaag gacctggccc tcgtgctgcg ctacggctcg ctgccggtgg agctcgaggc
      721 ccagcagacc cagtcgatct cggcctccat cggccaggac gccctcgacg ccggggtcac
      781 cgccggcctc atcggcctcg gcctggtcac catctacatg ctcgtctact accgcctgct
      841 cggcctggtc gccatgctgt cgctgtcggt ctccgccgcc ctgctgtgga cgatgatcgc
      901 ctacctcggc gagaaccagg gtctggccct caccctcgcc ggcgtcaccg gcatcatcct
      961 ctccatcggc gtggccgtgg actccaacgt ggtgttctac gagcacctca aggaggaggt
     1021 ggcgagcggc cggtcgctgc gcgccgccgt cgacggctcc ttcgccaccg cgttctccac
     1081 catcgtcaag gccgatgtcg cctccctcat cggcgctgcg atcctgtact ggctcaccgt
     1141 gggcccggtc cgcggcttcg ccctcttcct cgggctgtcc acgctgctcg acctggtcgc
     1201 ctcgtacttc ttcatgcggc ccctcaccgt gctgctcgtg aagtccgagc ggctgcagca
     1261 ccggccccgc ctcttcggga tgcccacacc caaggtccac cgggcaccca ccatcgacga
     1321 aggagcgccg gcatgagcgt ccgcagcaag ctcgccagct tcgacaacga catcgacttc
     1381 ccccgcctct ggcgcatcgg cgcggtcatc tccctcgtgc tgctgctgct cagcggcggc
     1441 gcgctgctct tccgcggcct gaacctcggc atcgacttcg agggcggcac ggcctgggag
     1501 gtcgacgccc ccgacgcctc ggtgtccgag gcccgcgacg tcctgacgcc cctgggcctc
     1561 ggcaacgcca agatccagtt cgtcggcacc gacaacctcc gggtgcaggc cgaaccgggc
     1621 gatgacgccc gcctggacga ggtcaccacc gagctcgccg agctcgccgg cgtcgaccgg
     1681 tccgaggtcg ccatctccac agtcggcccg acctggggag acgagatcct ctccaaggct
     1741 caacgggcgc tcgtggtgtt cctggtgatc atcgccgcct acatggcgat ccggctggag
     1801 tggaagatgg cggtgggcgc gctcgccgcc ctcgtccacg acatcatcat caccgtcggc
     1861 ttctacgccc tgttccagct cgaggtgacg ccggcgacgg tcatcgcctt cctcacgatc
     1921 atgggctact cgctctacga cacgatcgtg gtgttcgaca aggcccaggc caacgccgag
     1981 cggccggcgc tcgcgcagaa gatgaattac accgacctga tgagcctgtc gctcaaccag
     2041 gtgctgatgc gctcgctcaa caccaccatc acctcgctgc tgccggtgct gtccatgctg
     2101 ctcgtcggct cgttcctgct cggtgcggtc accctccagg agttcgcctt cgccctcgcc
     2161 gtcggcctcg ccatcggtgc ctactcgtcg gtgttcgtgg ccacgcccgc cgtcgcgtgg
     2221 ctgaaggagc gcgagcccca ccagcagaac ctgcgccagc gcctcgacgc ccagggcggt
     2281 cgccgcagcg gcgagccggt cacggcgggc gcaacgacgc ccgaatcccg cccggtcggc
     2341 gcggcgcccc agctgtcggg ccgggccatc ccgccccgcc cccgcaagaa gggcaagcgc
     2401 cgctgatgtc ggacctccca ccgccgccac ccccgccgcc tccgccctcc gggccgccgc
     2461 ccgggtccgt tccgggtccg gcggcgggcg gtcagctcac gccggtgggc ccctggcccc
     2521 gcttcggtgc ccgcatcatc gacggcctcg tgctgttcat ccccaacgcg atcatcgccg
     2581 ccgtgctgac cgacgacacc gccaccagct tcggcggcgt cggcaccggc gagaacttcg
     2641 cctacctcct ggtgagcacg ctcctcggct tcgcctacgc cgtctggttc gaggcatccc
     2701 ggggccagac cgtgggcaag atggccctcg gctacaaggt ggtcggcccc gacgggcggg
     2761 tcccgagcac cgacgtcgcc atgcgccgca acgcctggct gctgctcggc ctcgtgccca
     2821 tcgtcggggg cgtgctgtcc ttcgtcgccg cggtggccat cggcgtgacc atcgccaacg
     2881 acccgttcaa gcggggcacg cacgacaact tcgccggcgg ctccgcggtg gtccgggctc
     2941 gctagcccta tcctgaagcg gtgccgtcca cggtcgatcg ggtcctgccg tggcgccgga
     3001 gcagtccgcc gccagcagaa gaggtcgcgc cgctcctgac ggccttccgg gcccggcacg
     3061 gccggaccgc ccgcaccgat ctcatcaccc gggcctacct gtcgtcgtcg gcggcccacc
     3121 acggccagct gcgccagacc ggggaggcct acatccacca cccgatcgcg gtggccaaga
     3181 tcgtggccga cctcgggttg gacgacgtca ccatcgccgc cgccctgctc cacgacgcgg
     3241 tcgaggacac ggccatgtcg ctggccgacg tggaggagga gttcggcgcc gaggtggcgg
     3301 ccatcgtcga cggggtcacc aagctcgacc ggatccactt cgacaccaag gagcagcagc
     3361 aggccgccac ggtgcgcaag atgatcgtgg ccatgtccaa ggacctgcgg gtcctgatca
     3421 tcaagctggc cgaccggctg cacaacatgc ggaccatcgc cggcatggcg cacttcaagc
     3481 aggagcgcac cgctcgcgag accctcgaca tctacgcccc gctggcccac cggctcggca
     3541 tgcaggaggt gcgccagcag ctcgaggacc tcagcttcgc cgccctgcac ccgaagcgct
     3601 tcgccgagat cgaccacatg gtctccaccc ggacgcccga gcgcgacctg tacctgcacc
     3661 aggtgatcca ccaggtcgag gagcgcctcg aggagctgcg gctcaccgcc gaggtcaccg
     3721 ggcggccgaa gcacatgtgg agcatctacg agaagatggt cgtgaagggt cgcgacttcg
     3781 acgacatctt cgacctcgtg gccatccggg tcgtcgtgga ctcggtgaag gactgctacg
     3841 ccgcgctcgg ctccatccac gccacgtgga agccggtgcc cgggcggttc aaggactaca
     3901 tcgcgatgcc caagttcaac ctctaccagt cgttgcacac gacggtggtc ggaccgcagg
     3961 gcaagccgct cgaggtgcag atccgcaccc gggagatgca cgcccgcgcc gagcggggtg
     4021 tggcggccca ctggcagtac aagtcgaacg acaagcccca cgagcgcagc cacgacgacg
     4081 acctggcctg gctgtcgcgc atcgtggact ggcaggagga gtccaccgac cccgacgagt
     4141 tcatgaagaa cctgaaggtc gacctcgagc aggacgaggt cttcgccttc acccccaagg
     4201 gtcgggtcat cacgctcccg aagggcgcca cgcccatcga cttcgcctac accgtccaca
     4261 ccgaggtcgg gcacacctgc atcggcgcca aggtgaacgg ccgcctcgtc ccgctgcaca
     4321 gcgagctgag ctccggcgac acggtcgagg tggtcacctc caaggtcgag ggagccgggc
     4381 cgtcgcgcga ctggctgcag gtggtcgtca gcccccgggc ccgcaacaag atccggcact
     4441 ggttctcccg cgagcggcgc gaggacgcca tcgagaccgg tcgggaggac ctggtcaagg
     4501 cgctccgccg ggagaacgtg ccgatcaaga ccgagccggt cacggccgcc ctccaggaca
     4561 tcgccaacca ggccaactac gccgacctcg actcgctcta cgccgccatc ggcgagcacc
     4621 acctgtccgc caagtccgtg gccggccggg tcgtcaaggc gctgcaggac accgacgtcg
     4681 tcgagcccga gcagctcgcc accaacctgg tccacgaccg ccggagccgc tcgcgtgccc
     4741 gcaagtccaa gacgggcgtg cacgtcgagg gcctcgacga cgtcatggtc cggctgtcgc
     4801 gctgctgcac gccggtgccg ggtgacgaga tcgtcggctt catcacccgg ggtcgcggcg
     4861 tgtctgtgca ccgcgccgac tgcgccaacg ccgtgtccct cggcacgggc cagcgggacc
     4921 gcatcatcga cgtggagtgg gacggcgact ccgccggcgt ctacgtcgcc tccatcgagg
     4981 tgaaggccct cgaccgctcc cgcctgctgt tcgacgtcac ctcggccctc tcggaccacc
     5041 acgtgaacat cctggcgtcc tccaccacca ccggcagcga ccgcgtgtcg accatgcgct
     5101 tcgagttcga gctcggcgat cccgcccacc tcgactcggc catctccagc atccgggcca
     5161 tcgactccgt ctacgacgcc taccgggtcg tgcccggcaa gggcggctga gcacccgccg
     5221 gggcgagctg ctgctcagac gacgcgggac tcgtcccggc cggcgaagtc gagccggatc
     5281 cgggcttcca cgtcctgcgc gtcgaggctc tgcgcccccc gttgcagcgc cggctcgacg
     5341 tcctcgagca ggcggtcggg atctgcgtcc cgatcgaccg tgagccgcac ccgcatcgtc
     5401 ggctcctgac ccagcgagcg gatgcgcacc gctgcagagc ggaccccgtc gacggtctcc
     5461 agatcgtggg ccatcgcctt ggcgacggcc acgggctcca gggtcgtgcg tccccggcgg
     5521 ccggcggcga ccaccgtggt ggacaggtgc gggccgctgg gccggctggc gagctgccgg
     5581 aacagccaca ccagggcgag caccagcacc aggccgctca cgccgagtgc gacggcgtac
     5641 cagaggtcgg ggtcggcgac gacctcggcg cgcacgtcgc cgtacacggt ggcgggggag
     5701 tccaggtcgt cgaaggtgcc ctccccggct gcgaggccga gcgccccggc ggccgccagc
     5761 agcaggccga ggacgaggag gaggaagcgg ttcagtccgt cgatcacgtc agatcacccg
     5821 atcgtcggtg tgggcggcat ccacccgcac gcgttgcttc ttggccaggc gcagtcggtc
     5881 gagctcgtct cggaccacgg tgcgcaccga ctgctcggtc gggtcggggt cggccccgtc
     5941 ggggatccga gccgtcacct tcgtcttgcg cttggagacc ttggccttgc cgccgagcac
     6001 gccctcctgg tcgaccacga ggtcgcgcag ccgctcgtcc agcccgcttc ggccgaagcg
     6061 cacctcgcgc tcgccgtcgg cgcgccgccg gtcgagggtg gtcgtgcggt gggggatgag
     6121 ctggagcacc acgaggagca ggcccacgcc gagggcgatg gccgaggcga tggtccaggc
     6181 gtccgccccc cacgtggtcc agtcgcccgc ggtgagccgc tgctcccagt cgtcgatggg
     6241 gaggagcacg gggctctcgt cgatgcggat gcccaccgac tcgacgatga cgaggacggc
     6301 ggtggccgcg agcgcgaggg ccaccacgaa ggcgaggaag cggcgcagca gggtcatggg
     6361 gctcctgggt ggtcgagctc ggcggcttcg ctgacgatgt cagcgatgtg gacgtggacc
     6421 tccggttcgg cggcgtccaa ccgggcggtg acccgggagc ggacctcgtc gccgatgtcg
     6481 gggagcgccc gaccgaagcg cacgaccaag cggacctgca ccttcgtgcg cccgtctcga
     6541 tcgcgctcga cccggacgcc cgggacccgg ccgccgagcc cgtaggtcgc gaactcaccg
     6601 gccagccccg cgtcgaggtc gaccacgtcg tcgtgggccc gcgccgcgtc ggcgacgatc
     6661 cgggcgacgt ccacgggtgc cggcggccgc gggtcggcgt cggcgtcggc gggcatgggc
     6721 tactcgaccc gaccggtgtt ctggtcctcg tcgccgcccg ggaaggcgag gtcgttcacg
     6781 gcgatgttca cctcgacgac ctcgagcccg gtcatgttct cgatgcggtc gatgacgttc
     6841 tggcgcaccg cgccggtgac gtccatgatc gacgccgggt agcggaccgt catcgacagg
     6901 tcgacggcgg cctgcttggt gccgacctcg acgccgacgc cggcggtctt gtgctcgtcg
     6961 ccacggatgc ggccgacgac gctgctgagc gcaccggaga tggagccacc gagggactcg
     7021 acgccttcga cctcccgggc ggcgatggcg gcgatcttgc cgacgacgtc ctcggagatc
     7081 gaggtgacgc ccttgtcggt ctcgaggcgg gcgagcgagc cgctgccgcc gccgctgggc
     7141 gaggggcggt tcgtgctcgt gctcggggtc tgcgtgctgg tgctggtgtc agtcactggt
     7201 ggactctcct tgggtggtgg gcagggaaga cggggtgtgg ctaggtgtcg cggtcctggc
     7261 gcaggacgtc gcggacgttg acgccctgtt cgatgacgat gccgaggagg agccccacgg
     7321 caccgagacc cagcgtcagc agcgcgccat cgaggccggt gaaggcccag acgccgccga
     7381 cgacgaggcc gacgatgatc cagaagcgga ggttgctttg catgagggaa cctttcgggc
     7441 tcaacggacg cgggtgggtc ggcgggggag gagacgggtg aggagggcgg agaggcgatc
     7501 tcggcgcttg ggggcgggga ccaccacgac gcggcgcgcc gcgtgggtgg atcgcgtggt
     7561 ctcgccgtgc aggcagcgcc gggcgtcgag gagcagctgg gcgcggccgg tgtcgccgcc
     7621 gtgatccggg tgcgagacgc gcatgccgcg ccggaaggcg cgctcgatct cgtcggggga
     7681 cgcatcggtc ggcacggcga ggatggcgcg cgcccggtgc ggcgtcatca cggctgcctc
     7741 gtacggggat cccacgtctg ctcgctaccc cggagatccg ccatcatgcg gacgagcagg
     7801 agattccgcg ccgagaaccg cccaacagag gacgatcgcc gcgaacttcg acgttcgtgg
     7861 ccggtcccgg aagccaactc ggtggtccgg ggggcagcca ctaccctcag ccgccgtgtc
     7921 cacgcccgcc ttctccgccc cgatcggcac gcgcgacgtg ctcgcgccgg agtcggcccg
     7981 ctggatcgcg ctcctcgcca cgttcggtga gatcgcccag cgttacggct acgggatggt
     8041 gcacacgccg atgttcgagg agctcggcgt gttccagcgc atgggcgcca ccaccgacgt
     8101 cgtcaccaag gagatgtacg acttcgagga caagggcggt cgccgcatcg cgctgcgtcc
     8161 cgagggcacg gccagcgtcg tgcgcgcctt cgtcgagcac cggccgctgg tgccgtggaa
     8221 ggcctggtac gtcacgccgg cgttccggta cgagaagccc cagaagggcc gctaccgcca
     8281 gcaccaccag gtcggcgtcg aggtgctcgg cgtggacgac cccgacgtcg acgtcgaggt
     8341 catcgcgctg gccgagcgct acctgcagtc cctcggggcc tcccgctacc tgctcatcat
     8401 caactcgatg ggcgacgccg aggaccgggc caactacatg gagatcctgc ggggctacct
     8461 cggcgacgtc cgggagcagc tcgccgagga ggaccgcgag aaggtcgagc gcaacccgat
     8521 gcgggtgctc gactccaagc gccgcgagac gatgcaggcc acggccgacg cgccgcgcat
     8581 ctccgatgtg ctgtccgccg agagcgtcga acggttcgag cgggtgcagg ccgggctgcg
     8641 ggccctcgac atcgagttcg ccatcgaccc ccggctcgtc cgggggctgg actactacac
     8701 ccacaccctg ttcgagttcc agagcaccgc gctcgacgcc gcccagtcca cgatcatcgg
     8761 cggcggtcgc tacaacggcc tcgtcgaggc gctcggcggg cccgagaccc ccggcatcgg
     8821 cttcggctcg ggcatcgagc gggtgctcct gcacctcgat gccgaggacg ccttccccac
     8881 gccggacacc ggcgtcgacg tcttcgtgat cgacaccacc ggcggccacc acgcccgcga
     8941 catcgccgag gagctccgcg accacggcct gtcggcggac cgggcgtggg acgaccggtc
     9001 catgaaggcc cagatgaagg cggcggatcg ctccggcgcc cggatcgccg tcatcgtcgg
     9061 cgccgacgag gccaccgccg gccacgccac catccgcgac ctgcgcggcg atcggggcca
     9121 gcgctccgtc gcccgccccg acctcgtcga cgaggtcgcg gccctcctcg cccccgatcc
     9181 ccaagtggag accccgtgac cgagatcctc tcgaccggac cagccgtcgg cctgcgcacc
     9241 cactggtgcg gcgagctgcg cgccagcgac atcggcgcca ccgtgtcgct ctgcgggtgg
     9301 gtggagaacc gtcgcgagca cggcgagcag ctggcgttca tcgacctccg cgaccgcacc
     9361 ggcatcaccc agtgcgtcgt ggccaaggcc cacgacctgc gcggcgagta cgtgctgcgc
     9421 atcaccggca cggtgcaggc ccggttcgag ggggccacca acgacaagct cgacaccggc
     9481 gagatcgagc tgcaggactg caccgtcgag gtgctctcca catccgagcc ggcgccgttc
     9541 ccgatccacg agcgcaccga ggtcgacgag accatccgcc tgcgccaccg ctacctcgac
     9601 ctgcgccggg cccgcctgca gaagaacctg caggtccgca ccatcgtgaa cgccgccatc
     9661 cggcgcacgt tcatcgacaa cggcttcgtc gaggtcgaga cacccatgct catcgcgtcg
     9721 acgcccgagg gcgcccgcga cttcgtcgtg ccgtcgcgcc tgcacccggg cagcttctac
     9781 gccctgcccc agtcgcccca gctgtacaag cagctctgca tggtgggcgg cgtcgaccgc
     9841 tacttccaga tcgcccggtg cctgcgcgac gaggacctgc gcgccgaccg ccagttcgag
     9901 ttcacccagc tcgacatgga gtgctccttc gtcggtcagg aggaggtgct cgacttcgtc
     9961 acccagtcgg tgcgagccgc cgtggccgac atcaccggcg acgacatcgg cgacgtcccg
    10021 cgcatgacgt gggaggaggc gatggagcgc tacggctccg acaagcccga cacccgcttc
    10081 ggcatggagc tggtcgagct gaccgagctg ttcgccgaga ccgaggccaa cgtcttcaag
    10141 gccccgtgcg tgaagggcat ccgcctcgag ggcggtgccg agctcacccg caagcagatc
    10201 gacggcctcg tggaccaggc caagctgtgg ggggccaagg gcctcgcgtg gatgcgggtc
    10261 gtctccggcg agcgggagat gccggcgctc gatggcggcg tcgccaagtt cctctccgag
    10321 accgagtcgg tggccaccat caccgcgctc gacgccgcgc cgggcgacat ggtgttcctg
    10381 gtcgccgacg agcgccccgt ggcccgccac gtgctcggcc tcctgcgcgt cgagctgggc
    10441 aagccgccga tggacgaggg cggcctcgag ttcctctggg tcaccgactt cccgatgttc
    10501 gacgacgagc tcggccccga cggccgcccc caggccatgc accacccgtt cacgatgccc
    10561 aacgccgagg acctcgaagc cggcctcatc gaatccgacc ccaccaaggt ccgctcggtg
    10621 gcctacgacc tggtgtgcaa cggctgggag ctcggctcgg gcagcgtccg tatccaccgc
    10681 agcgacgtcc agcagcgggt gttcgacgcg ctcggcatcg cccaggagga ggcccaggag
    10741 aagttcggct tcctcctcga cgccctccgg ttcggtccgc cgccgcacgc cggcttcgcc
    10801 tacggcatcg accggctcgt ggcgctcctg gccggcgaga ccaacatccg cgaggtcatc
    10861 gccttcccca agacccagaa gggcgccgat cccctcaccg acgcaccgag cgagctcgag
    10921 ccccgggtgc tcgaggagct gggtctgcgg gtcctgcccc gcctggacta gctacgcgtc
    10981 ttcgaggccg tcgaggaacg agcacagggc gcggcgggcg cgctcgcgct ggtcggccag
    11041 cgtcttgcgc ccggccacgg tgccgacggc accgagggcg ccagcgagcg gcagggaggc
    11101 gaaggccacc cacgggtctg tgagcacggc ggcggcgacc ccgaggaccg gagtgaccgc
    11161 cgtcgggatg accacgaccc ccgtgcgcag gccccggcgc atgtcgtcga acacgacctc
    11221 gaggcgcacg aggctgcggc cctcgccgca gtccacgacc gagacctcga cggcgtcgac
    11281 gtcgccgagg cgcacccggc gtgcggtgcg ggcatcgatc ttgcgccgga gcttggcgcc
    11341 gaggtcgtcc cgcggccgcc acacctcggc ggagccctgg cgctcgtcgc ggtggaggag
    11401 ctggctccgc agccagtggg tgacgtgccc gcgggactcg gccacggtcc gcggcacgac
    11461 gcgcgtctcc agcacccggg acgggccgag caggccgccg cgggcgtcgg tgtgggccag
    11521 gatcccggcg tcgtgctcgg ccagcgccag ctccacggcg ccacgctcga tgccgacctc
    11581 cgacgccgct tcggtcaggg cgcggcggtc gtagccgctg tgggcggggg ccggacggtc
    11641 ctgcgcgctg atctcggcgg cccggcggag cacctcgtcg gccgcgagcg ggtccagcgg
    11701 ctcggtcatc gggcgctctc ggtcaccgca ccatcctgcc cgtagcctcg gcgccgtgca
    11761 ggtccaccac ctccgatcgg tcgacggcga gatcgtgtac gacatggatc ccgagatcgt
    11821 gaccccggcg gcgggcggca cccgcatggt ggacgacgtc acggtggacg agctggccct
    11881 gctggcccgg gccatgacct acaagctcgc cgtgttcgag ggtcgcatgg gcggcgccaa
    11941 ggccggcatc cgcccccgcc tgctggacag ccgggccgag accatcaccc ggtactgcga
    12001 cgagatccga ccactcgtcg agtccgggcg cttcttcacc ggcgccgacc tcggcaccac
    12061 caccgccgac ttcgccgacc tcggggacga cacccctgcg gtgttccgcg aggtcatcga
    12121 cggcgtcggc ttcgaggagc tggtgaccgg gcggggcgtg gtcgccgcag cggcagcctg
    12181 gctcggcacc ctcgaagatc gcaccgtcgc gatcgagggc ttcggcaagg tcggccacgg
    12241 cgtggcccgg gaggtgctcc gccggggcgg tcgggtggtg gcggtgtcga ccctgcgggg
    12301 cgcggtcgcc aaggctggcg ggttcgacgt cgacgagctc gtgcaggcac gcgccgagca
    12361 cggcgagtcc ttcgtccacc acctcgggct cgacgtccac ctgccccggg agctgcacga
    12421 gctgcgcgtc gacgtgctcg tcccgggtgc ccgggtgggc gtgtacgacg ccgcgctggc
    12481 tgcccgggtc caggccgagg tcatcgcacc ggcggccaac gtcccctaca ccgtcggcgg
    12541 tctggcggag ctgcgagccc ggcgggtggc ggcactgccc gacttcgtct gcaacggggg
    12601 agcggtgctc gcctaccaga cctcgggcgc gctgacgccg gccgaggtgc tggcccgggt
    12661 cgaccgggag atcggcgagc gcatcgaggc cgctcgcctg gcgaagatgg acccgatccg
    12721 ccacgccgcc gtgctggccg agacgttcct cagcacctgg gtgcccgccg accagcgccc
    12781 cgacggcccg gcggtggccc cgtgagcgac gacctcttcg ccgcggcggc ctcgtcccgc
    12841 ctcgagtccc gtcagcccct cgccgcccgc ctgcgccccc gctcgctcga cgacatcgtc
    12901 ggccaggagc acctgctcgg cccgggcaag ccgctccgat ccctggtcga gcgcgaccgg
    12961 ctgtcctcag cgctgctgtg gggcccgccc ggcaccggca agacgacgct ggcccgggcg
    13021 gtggccgccc acaccgagaa ggtgttcgtg cagctctcgg cggtcacggc cacggtcaag
    13081 gacgtgcggg agacgatcgc ccaggcccgg caccggctcg gcgagcaagg gcggggcacg
    13141 atcctgttcc tcgacgagat ccaccggttc aacaaggccc agcaggacgc cctgctgccg
    13201 gcggtggagg agggcctggt ggtcctggtc ggcgccacca ccgagaaccc gtacttcgag
    13261 gtgaacgcgc cgctgcgcag ccgatccacg ctgttccggc tcgagccgct caccaccgac
    13321 gcggtccgca ccctgctcgg caacggcttg gaggccgagg gggcgaccgc caccgacgag
    13381 gccatcgagc acctggccga gcgggccaac ggcgacggcc gccaggccct caccgcgctc
    13441 gaggtggccg tcgccctcgc caccgctcgc gccgaggccg gcgaggggag cggggggaac
    13501 gaggggaacg agggcagcgg tcgacccgag gtcacgctgg ccgacgccga ggccgccctc
    13561 ggcaccaacg ccctgcggta cggggtcgac gaccactacg acgtggtgtc ggcgttcatc
    13621 aagtcgatcc ggggcagcga ccccgacgcc gggctctact ggttggcccg gatggtcgag
    13681 gccggcgagg acgcccgctt catcgcccgg cgcctggtga tcctggcctc ggaggacatc
    13741 ggcatggccg accccaccag cctcctggtg gccaacgcgg cagcgcacgc cgtcgagttc
    13801 gtcggcctgc ccgaggccgg gcacaacctg gcccaggcgg tcgtgcacct gtcgtcggcg
    13861 cccaagtcga accgggtcgc caaggcctgg tgggcggcgc agtccgacgt gcgggaccag
    13921 ccggccggcg aggtgccggc ccacctgcgc gacgcccact accagggggc cggcgccatc
    13981 ggccacgggc ggggctaccg ctacccccac gacgacccga ggggctgggt cgagcaggag
    14041 caccggcccg agggcgtggc cggccggacg tactacgaac cctccgagca cggcgccgag
    14101 gccgaggtcg cccggcgcca gcgggagcga acgcctcggg gcggctgacc ctccacggat
    14161 aggctcggcg gggtgagagc agacgaactc cgccgcgcct ggacacagtt cttcgtcgac
    14221 cgcggccaca cggcggtgcc ctccgccggt ctgatcccgc accacccgtc tgccccgatg
    14281 ttcgtgaact cggggatgat gccgttcgtg ccgtacttcc tcggcgagga ggcgcccccg
    14341 ttcgacccgg cgcgggccgt ctcggtgcag cgctgcgtcc gggccggggg caagcacaac
    14401 gacctcgacg ccatcgggcg ctcgcgccgg cacctcagct tcttcgagat gctcggcaac
    14461 ttcagcttcg gggactactt caaggaagac gccatcccgt gggcgtggga attcgtcacc
    14521 gaggtgctcg gcctcgacgg cgaccgcatc tggatcaccg tccacacctc cgacgacgag
    14581 gccgaggaca tctgggccga cgccgtcggc ttcccgcgcg agcgcatcca gcgcctcgac
    14641 aaggacaact tctgggag
//
LOCUS       DATISU010000096         4194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_97787, whole genome shotgun sequence.
ACCESSION   DATISU010000096 DATISU010000000
VERSION     DATISU010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97787"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VLR27_04160"
     CDS             complement(<1..961)
                     /locus_tag="VLR27_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02668.1"
                     /translation="MPITAPTVVRAATALLPGHATGELAGPVEVELRDGRIVDVRPGS
                     DPGDGSVLAPGFVDLQVNGFDDVDVAIADGADWDRLDRLLAATGVTAWLPTLVTQRLE
                     RYGPALERIAAASARGGSGPAVLGAHLEGPFLGQHTGAHDPRRVVPIDLEWLAALPDV
                     VALVTLGAEQHEAVAAIEALTARGTVVSIGHSGATAERAAEAFDAGATMVTHLFNAMG
                     PLHQREPGVPGAALADDRVVAGLIADLVHVHPALIRTAFRTKGAHRIALVTDAVAWRA
                     GHLAAADVTLVDGAPRLADGTIAGSALTMATAVRNVVDAAGVPL"
     gene            1040..1645
                     /locus_tag="VLR27_04165"
     CDS             1040..1645
                     /locus_tag="VLR27_04165"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP02669.1"
                     /translation="MSDRDPGSTREAILDAARTAFAERGYAGASLGDIAKSVGIAKAS
                     LLHHFSNKDELYREVFERLLAQWFVRVEEAVEGPQEGWTQFDHVLSAGFEFFAENPDL
                     VRLVRREALDGGHFGVDLGAALRPMFEQAVGYLEAQMKDGRFRHHDPEQLVISGLGAV
                     LSYFSDLPFIEGLLGRDPLAVEELVRRQDHIREFFRAALEP"
     gene            complement(1675..2979)
                     /locus_tag="VLR27_04170"
     CDS             complement(1675..2979)
                     /locus_tag="VLR27_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSP02670.1"
                     /translation="MTEQDDVLDELAALRMLIENSTDMLSRHTPTGTYRYVSPASREL
                     LGYEPEELLGRSAYDLFHPDDLAAISANHGEVLEQPDLRTIVYRIRHRAGHYVWFETT
                     SHAVRDPGSGEIVEIQTSSRDVTRRVETETRLRRSEQQFRLAMEHAPTGMALVGLDGS
                     FLEVNDQLGNVVGRTAAELRALRFQDITHPDDLDADLAYVQQLLEGEIDRYSMAKRYI
                     HADGRMIWVLLSGSLVRDADGAPHHFVAQIQDITQQIVQEERLRAANEALERLVNEDL
                     LTGLATRRAVLRGLDSELTRDDRHGRGVAVMLADVDRFKRVNDRHGHAEGDRVLRDIA
                     SAVSGAVREVDLVGRFGGEEVLVVLPEVAEPAALSIAERVRAHICSSVSSPDGPVTVS
                     IGVAVRRPAESAEQLIARADQALYRAKRAGRNNVQGQHPAQA"
     gene            3137..>4194
                     /locus_tag="VLR27_04175"
     CDS             3137..>4194
                     /locus_tag="VLR27_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HSP02671.1"
                     /translation="MPTLSEADSKALLASHGLPVAGERQVTTADEAASAAGELGFPVV
                     CKLGGDSIAHKTERGLVRLNLPDAAAVRSAATELLDAARPEDGEVHVLVAPMVKGNRE
                     LIAGLHLDPQFGMTVMLGVGGILAEAVADVAFRLAPLTERDAFDMIDSLETQALLGPF
                     RGEDAVDRDQLAAVLLALSRAAEADPRIVAADLNPLIVTADGTPIAVDALVELTEEGT
                     ERADGPQPETTERPTPEQFRALFEPRGIIIAGASTHPGKFGFVTLHNLLAGGYSGQIF
                     ATNREGSEVLGVPTVPDVTDLPEGEADLIFLCTPAKANPQILRDAASIGVKAAFITSA
                     GYGEAGEEGLKAQEELKAL"
BASE COUNT          552 a         1538 c         1499 g          605 t
ORIGIN      
        1 cgagggggac gccggcggcg tccacgacgt tgcgcaccgc ggtggccatc gtcagcgcgc
       61 tgccggcgat ggtgccgtcg gcgagccgcg gcgcgccgtc gacgagcgtc acgtctgcgg
      121 ccgcgaggtg gccggcccgc caggcgaccg catcggtgac cagggcgatc cgatgggcgc
      181 ccttggtgcg gaacgcggtg cggatgaggg cagggtggac gtggacgagg tcggcgatca
      241 gcccggccac gacgcggtcg tcggccagcg ccgcacccgg gacgccgggc tcgcgctggt
      301 gcagcggtcc catggcgttg aacaggtgcg tgaccatcgt ggcgccggcg tcgaaggcct
      361 cggcggcccg ctcggcggtc gctccggagt ggccgatgct gacgaccgtg ccccgggcgg
      421 tgagggcctc gattgccgcg acggcctcgt gctgctcggc gccgagggtg accagagcga
      481 cgacgtcggg cagcgccgcc agccactcga ggtcgatggg cacgacgcgg cgggggtcgt
      541 gggcgcccgt gtgctggccg aggaaggggc cctcgaggtg agcgccgagc accgctgggc
      601 cggatccgcc gcgtgcgctc gcggcggcga tgcgctcgag cgccggtccg tagcgctcga
      661 gtcgctgggt caccagggtc gggagccacg ccgtcacccc ggtcgcggcg aggagccggt
      721 cgaggcggtc ccagtcggcg ccgtcggcga tggccacgtc cacgtcgtcg aagccgttga
      781 cctgcaggtc cacgaagccc ggtgccagca cgctcccgtc accgggatcc gaccccggtc
      841 ggacatcgac gatgcgccca tcgcgcagct cgacctcgac cggtccggcc agctcaccgg
      901 tggcgtgacc gggcagcagc gcggtcgcgg cccggacgac ggtcggtgcg gtgatgggca
      961 cggcgggacg gtaccggagg gtcgtcggcc gtcgaaccgt cccgggggtg gcatcccggt
     1021 gggcagtagc ctcccggcgg tgtccgaccg agatcctggg tccaccaggg aagccatcct
     1081 cgacgcggcc cgcacggcct tcgccgagcg cggctacgcc ggggcgtcgc tcggggacat
     1141 cgccaagtcg gtcgggatcg ccaaggcgag cctcctgcac cacttctcga acaaggacga
     1201 gctctaccgc gaggtgttcg agcggctcct ggcccagtgg ttcgtgcggg tcgaggaagc
     1261 cgtcgaggga ccgcaggagg gctggaccca gttcgaccac gtcctgtcgg ccggcttcga
     1321 gttcttcgcc gagaaccccg acctggtgcg cctcgtgcgg cgcgaagcgc tcgacggcgg
     1381 ccacttcggc gtcgacctgg gtgccgccct gcgcccgatg ttcgaacagg ccgtcggcta
     1441 cctcgaagcc cagatgaagg acggccggtt ccgccaccac gaccccgagc agctggtcat
     1501 cagcggtctc ggcgccgtgc tgtcgtactt ctccgacctg cccttcatcg agggcctcct
     1561 cggccgggac cccctcgccg tcgaggagct ggtccgccgc caggaccaca tccgcgagtt
     1621 cttccgcgcc gccctcgagc cgtaggcggt caccgcccat gcgctgcctc gccgtcaggc
     1681 ctgcgccggg tgctgcccct ggacgttgtt ccgtccggcg cgcttggccc ggtacagggc
     1741 ctggtctgcg cgggcgatca gctgctccgc cgattcggcg ggacgcctga ccgcgacacc
     1801 gatgctgacc gtgaccgggc cgtcgggtga ggacacgctg ctgcagatgt gggctcggac
     1861 ccgctctgcg atgctgagcg ccgcgggctc cgccacctcc gggagcacca cgaggacctc
     1921 ctcgccgccg aaccgtccca cgaggtccac ctcgcgcacg gcgccggaga ccgcggatgc
     1981 gatgtcccgc aggacacggt caccctcggc gtggccgtga cggtcgttca cgcgtttgaa
     2041 gcggtccacg tcggccagca tcacggcgac gcccctgccg tgccggtcgt cccgggtcag
     2101 ctcggagtcc agtccccgca acaccgcccg tcgtgtcgcc agaccggtca gcaggtcctc
     2161 gttcaccagt cgctccagcg cctcgttcgc ggcccggagc ctctcctcct ggacgatctg
     2221 ctgggtgatg tcctggatct gggccacgaa gtggtgcggt gcgccgtcgg cgtcgcggac
     2281 gaggcttccg ctcaggagca cccagatcat gcgtccgtcg gcgtggatgt agcgcttcgc
     2341 catcgagtag cggtcgatct cgccttcgag cagctgctgg acgtacgcga gatcggcgtc
     2401 gaggtcgtcc gggtgggtga tgtcctggaa gcgaagggct cggagctcgg cggcggtgcg
     2461 accgacgacg ttccccagct ggtcgttgac ctccaggaag gagccgtcca ggcccaccag
     2521 cgccatgccg gtcggcgcgt gctccatcgc cagccggaac tgctgctctg atcgccggag
     2581 ccgggtctcg gtttccaccc gtcgggtcac gtcccgggag ctggtctgga tctcgacgat
     2641 ctccccgctt cccgggtcgc gcacggcgtg gctggtggtc tcgaaccaga cgtagtgccc
     2701 tgcgcggtga cggatccggt agacgatggt gcgcaggtcc ggttgctcga gcacctcacc
     2761 gtggttggcg ctgatcgccg ccaggtcgtc ggggtggaag aggtcgtagg ccgaccggcc
     2821 gagcagctcc tccggttcgt agccgagcag ctcgcgagac gcgggggaga cgtagcggta
     2881 cgtccccgtc ggcgtgtgtc gcgagagcat gtccgtcgag ttctcgatca gcatccgcag
     2941 cgctgccagc tcgtcgagca catcgtcttg ctcggtcatc atccacatcc cgtgtcgact
     3001 gcgacgtggg ccccagcgcc ccgccgcggg cgtccggagc gtaccgacga ccatcagcat
     3061 gccgggcagg caccgggccg ccccgctgct ggtgggttcc gagaccgcac ggggtccgcc
     3121 actacggttg cccgccgtgc ccacgctctc cgaggccgac tccaaggccc tcctcgcatc
     3181 ccacggactg cccgtcgccg gcgaacgcca ggtgaccacc gccgacgagg cggcgagcgc
     3241 cgccggggag ctcggcttcc ccgtggtgtg caagctcggc ggcgactcga tcgcccacaa
     3301 gaccgagcgt ggcctggtgc gcctcaacct ccccgatgcc gctgccgtgc gcagcgcggc
     3361 caccgagctg ctcgacgcgg cccggcccga ggacggcgag gtccacgtgc tggtggcgcc
     3421 gatggtcaag ggcaaccgcg agctcatcgc cggcctgcac ctcgacccgc agttcgggat
     3481 gaccgtcatg ctgggcgtcg gcggcatcct cgccgaggcg gtcgccgacg tggccttccg
     3541 cctcgccccg ctcaccgagc gggatgcctt cgacatgatc gactcgctcg agacccaggc
     3601 gctgctcggc ccgttccgcg gcgaggacgc cgtcgaccgt gaccagctgg ccgccgtgct
     3661 gctcgcgctg tcccgggcgg ccgaggccga cccccgcatc gtcgccgctg acctcaaccc
     3721 gctgatcgtc accgccgacg gcacgcccat cgccgtcgac gcgctggtcg agctgaccga
     3781 ggagggcacc gagcgcgccg acgggccaca gcccgagacc accgagcgcc cgacgcccga
     3841 gcagttccgg gcgctgttcg agccccgcgg catcatcatc gccggcgcct ccacccaccc
     3901 cggcaagttc gggttcgtca ccctccacaa cctgctggcc ggcggctaca gcgggcagat
     3961 cttcgccacc aaccgggagg gctccgaggt gctcggcgtc ccgaccgtcc ccgatgtcac
     4021 cgacctgccc gagggcgagg ccgacctcat cttcctgtgc acgccggcca aggcgaaccc
     4081 tcagatcctc cgcgacgccg cgtcgatcgg ggtgaaggcc gcgttcatca cctcggccgg
     4141 ctacggcgag gcgggggagg agggcctcaa ggcccaggag gagctgaagg cgct
//
LOCUS       DATISU010000097         3550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_144438, whole genome shotgun sequence.
ACCESSION   DATISU010000097 DATISU010000000
VERSION     DATISU010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144438"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /gene="nhaA"
                     /locus_tag="VLR27_04180"
     CDS             complement(<1..1221)
                     /gene="nhaA"
                     /locus_tag="VLR27_04180"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HSP02672.1"
                     /translation="MSERRLTTMRRPTWSEQDTFVPRLFVRPFQRFMHAEAGGAVFML
                     AAAVAALVWANSPWYHAQQTLWETDLSIHLGGLAHLDLTLVEWVNDALMAIFFFVVGL
                     EIKRELVHGELRDPKKAALPAIAALGGMLVPAAIFMVLNAGTAGFDGWGVPMATDIAF
                     AVAVVSLVGNRVPSSAKVFLLTLAIADDIGAILVIALFYTSNLSLGWLAIALGTLLVA
                     EICKRQHVRALPVYVGLALFAWFAFHETGVHATLAGVVLGLLTPAWPFYNPDNFSEDM
                     SSLLGHGTALDSIEQNLEQSRGSATYDDESYDEATSRLSEMERLVAETQSPLDRLENK
                     LHLWVAFLIVPLFAFANAGVRLSDEALDGLASNRVVLGIVAGLVVGKTVGVFTFTLLG
                     QKLGLGTLPAGVTQR"
     gene            complement(1273..1707)
                     /locus_tag="VLR27_04185"
     CDS             complement(1273..1707)
                     /locus_tag="VLR27_04185"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP02673.1"
                     /translation="MAEIVLEIPPRLDYLAVVRLVVATAASLDPPLPDSRLDDLRLAV
                     TEACSNAIKAHRPEAADQPVVISCHLDEDLFRVEIRDRGPGFDPDALARLPDPTDPSR
                     LQHESGLGIPLIKVLTDDVTFEPAGDGTIVSMTLKRPTAPPI"
     gene            complement(1717..2061)
                     /locus_tag="VLR27_04190"
     CDS             complement(1717..2061)
                     /locus_tag="VLR27_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSP02674.1"
                     /translation="MELEIPDTDVEGWTVVAASGEIDVATAPKLRDRLTELVEAGSTR
                     LIVDLEDVDFIDSTGLGVLVGAVRRARADAGDLRLVCTNTRILKVFEATGLHEVFTIG
                     STVDDAVAAEAQ"
     gene            2173..>3550
                     /locus_tag="VLR27_04195"
     CDS             2173..>3550
                     /locus_tag="VLR27_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSP02675.1"
                     /translation="MAGDRSDPCDLESLVRRRADQLLDRDSPHLADASRLVHVERLAA
                     TAPRYAEPATPLPPEVAERLGVEHLYTHQAEALDLVRGGRSVVTATSTSSGKSLIYQA
                     AIGEAALAPVRPGTALCLFPTKALAQDQLRSLAGLDLPGVQAATYDGDTGRAERGDAR
                     RNASVLLTNPEMLHCGILPHHAGWMRFLSRLRYVVVDELHLFRGIFGSHVAHVLRRLR
                     RIAAHYGADPVFICSSATIGEPAALASALTGVEVTAVTDDGSPSGERVVAIWNPPVLD
                     EDTGTRASTHGEAAGLVADLVRAERRTIGFCPSRRSTELVADRVRRQVPASRRRRVAA
                     YRGGFLAEERHEIEAALAEGALDAVVTTSALELGIDLEGLDACVLDGFPGTVASLWQR
                     VGRAGRRQQESLAVLVCGADQLDQWIAAHPEELFRRPPEPAVINPANPFVADPHLRCA
                     AFELPLTHD"
BASE COUNT          502 a         1244 c         1324 g          480 t
ORIGIN      
        1 gcgctgcgtg accccggcgg gcaacgtgcc gagcccgagc ttctggccga gcagcgtgaa
       61 ggtgaacacg ccgacggtct tgccgacgac gaggccggcg acgatgccga gcaccacccg
      121 gttggaggcc aggccgtcga gcgcctcgtc cgacagccgg accccggcgt tggcgaaggc
      181 gaacagcggc acgatgagga acgcgaccca gaggtggagc ttgttctcca gccggtcgag
      241 cggggactgg gtctcggcga cgagccgctc catctccgag agccgggagg tggcctcgtc
      301 gtagctctcg tcgtcgtagg tggcgctgcc ccgtgactgc tcgaggttct gctcgatcga
      361 gtccagcgcc gtgccgtggc cgagcaggga cgacatgtcc tcggagaagt tgtccgggtt
      421 gtagaagggc caggcgggcg tgagcagccc gaggaccacc ccggcgaggg tggcgtggac
      481 gccggtctcg tggaaggcga accaggcgaa cagggccagg ccgacgtaga cgggcagggc
      541 ccgcacgtgc tggcgcttgc agatctcggc caccaggagc gtgccgaggg cgatggcgag
      601 ccagcccagg ctcaggttcg acgtgtagaa gagggcgatc acgaggatgg ccccgatgtc
      661 atcggcgatg gccagcgtga gcaggaagac cttggccgag gacggcaccc ggttgccgac
      721 gaggctcacg acggcgacgg cgaacgcgat gtcggtggcc atcggcacgc cccagccgtc
      781 gaagcccgcc gtgccggcgt tgaggaccat gaagatcgcg gccgggacga gcatgccgcc
      841 gagggcggcg atggcgggca gggccgcctt cttggggtcg cggagctcgc cgtggaccag
      901 ctcgcgcttg atctcgaggc cgacgacgaa gaagaagatg gccatcagcg cgtcgttgac
      961 ccactcgacg agggtgaggt cgaggtgggc caggccgccg aggtggatcg acaggtcggt
     1021 ctcccacagc gtctgctggg cgtggtacca gggggagttg gcccacacga gcgcggcgac
     1081 ggccgcagcc agcatgaaca cggcaccccc ggcctccgcg tgcatgaacc gctggaaggg
     1141 ccggacgaac aggcgcggca cgaaggtgtc ctgttcgctc cacgtggggc ggcgcatggt
     1201 ggtgagacgg cgttcggaca tcgttgttcc tcggggcacg ggcgcccagg ggatccggca
     1261 gcggcgcgct gctcagatcg gcggggcggt gggacgcttg agggtcatcg agacgatcgt
     1321 tccgtcgccg gctggctcga aggtgacgtc gtcggtgagg accttgatca gggggatgcc
     1381 gagcccggac tcgtgctgga gccgggaggg gtcggtggga tcgggcaggc gggcgagcgc
     1441 atcggggtcg aagccgggac cgcggtcgcg gatctcgacc cggaagaggt cctcgtccag
     1501 gtggcaggag atgacgacgg gctggtcggc cgcctcgggt cggtgtgcct tgatggcgtt
     1561 gctgcacgcc tcggtgaccg ccaggcgcag gtcgtcgagg cgggagtcgg ggagcggcgg
     1621 gtcgagggag gcggcggtgg cgacgacgag tctgacgacg gcgaggtagt ccagccgagg
     1681 agggatctcg aggacgatct cggccaccgt ggtcgcctac tgggcctcgg ccgcgacggc
     1741 atcgtccacc gtcgaaccga tcgtgaagac ctcgtgcagg ccggtggcct cgaagacctt
     1801 caggatccgg gtgttcgtgc acaccagccg caggtcgcct gcgtcggccc gggcgcggcg
     1861 cacggcgccg accaggaccc cgaggccggt ggagtcgatg aagtcgacgt cctcgaggtc
     1921 gacgatcagg cgcgtcgaac ccgcctcgac gagctcggtg agccgatcgc gcagcttcgg
     1981 cgcggtggcg acgtcgatct cgccggaggc cgccacgacc gtccagcctt cgacatcggt
     2041 gtcagggatc tccagctcca tgcagtcgac cacgctaccg gtcggcgccc ggtcgactgc
     2101 aggccgccgg gatcacaggc gtggaactac aggcctgtcg tgatgtcggg gtgggggcgt
     2161 acgatcgccg aggtggccgg cgatcgctcc gatccctgcg acctcgagtc gctcgtgcgc
     2221 cggcgcgccg accagctcct cgaccgcgac agcccgcacc tggccgacgc gagccggctc
     2281 gtccacgtcg agcgcctcgc cgccactgcg ccccggtacg ccgagcccgc cacgccgctg
     2341 ccgccggagg tggccgagcg cctcggggtc gagcacctct acacgcacca ggcggaggcg
     2401 ctcgacctgg tgcggggcgg tcgctcggtg gtcaccgcca cgagcacctc gtcggggaag
     2461 tcgctgatct accaggccgc catcggggag gctgcgctgg cgccggtccg ccccggcacc
     2521 gccctgtgcc tgttccccac caaggccctg gcccaggacc agctccgatc gctcgccggc
     2581 ctcgacctcc cgggcgtgca ggcggcgacc tacgacggtg acaccggccg ggccgagcgt
     2641 ggcgacgccc gccgcaacgc cagcgtgctg ctcacgaacc ccgagatgct gcactgcggg
     2701 atcctcccgc accacgccgg ttggatgcgg ttcctgtccc gcctgcgcta cgtcgtcgtc
     2761 gacgagctcc acctgttccg gggcatcttc ggctcccacg tcgcccacgt gctgcgacgg
     2821 ctgcgccgca tcgccgcgca ctacggcgcg gatccggtgt tcatctgctc ctcggccacc
     2881 atcggcgagc cggcggcgct ggcatcggcg ctcaccggcg tggaggtcac cgccgtcacc
     2941 gacgacgggt cgccgtcggg ggagcgcgtc gtcgccatct ggaacccgcc ggtcctcgac
     3001 gaggacaccg gcacgcgggc ctcgacccac ggggaggccg ccgggctggt cgccgacctc
     3061 gtccgggccg agcgccgcac catcggcttc tgcccgagcc ggcgctccac cgagctggtg
     3121 gccgaccgcg tccgccgcca ggtgccggcc tcccgtcggc gccgggtcgc ggcctaccgc
     3181 ggcgggttcc tcgccgagga gcgccacgag atcgaggcgg ccctcgccga gggcgcgctc
     3241 gacgcggtcg tcacgacctc cgccctcgag ctcggcatcg acctcgaggg cctcgacgcc
     3301 tgtgtgctcg acgggttccc cggcaccgtc gcatccctct ggcagcgcgt gggccgggcc
     3361 gggcgccgcc agcaggaatc gctggcggtt ctcgtctgcg gcgcggacca gctcgaccag
     3421 tggatcgccg cccaccccga ggagctcttc cgccgcccgc cggagccggc ggtcatcaac
     3481 ccggccaacc cgttcgtggc cgacccccac ctgcgttgcg ccgccttcga gctccccctc
     3541 acccacgacg
//
LOCUS       DATISU010000098         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_284196, whole genome shotgun sequence.
ACCESSION   DATISU010000098 DATISU010000000
VERSION     DATISU010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284196"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1348
                     /gene="hisD"
                     /locus_tag="VLR27_04200"
     CDS             38..1348
                     /gene="hisD"
                     /locus_tag="VLR27_04200"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HSP02676.1"
                     /translation="MTGPLTTLDLRGTDTSDPHALRSVLPRPKAAEEPPIEQVRAVIE
                     RVREGGDDALRAMTKEFDGVDIDTIRVDPDDVARSVERIDPALRTALETAHGNIAAYH
                     RAQLHDPARYERDGLVVRELRRPVDRAGLYVPSALAPLLSTALMTAVPAKVAGVHETV
                     LCTAPGPDGAVDDGILAAAAIAGVDEVHRVAGPAAIAALAYGTESIRPVDVIVGPGSA
                     RVAQAKREVASSGLVGVPSSFAGPSEVVVVADAAAVVEDVAIDIVLQAEHGPDGLAWL
                     ICWDDEVRAAVDDAVARIVAASPRREHIEATLADAGYAVVVDGPTAAIDVANVIAPEH
                     LELQTADAEDLLVRVKHAGAVFCGPWAPASVGDYVAGPNHVLPTYGSARFAGALRVDD
                     FCKHIHVVSLDEAALRTLAPHVEAMAAAEGLAAHADSVRQRVER"
     gene            1345..>2013
                     /locus_tag="VLR27_04205"
     CDS             1345..>2013
                     /locus_tag="VLR27_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSP02677.1"
                     /translation="MKRPPVRDDLALHPGYHSPQVEVDVRLNTNEAPEAPPEGFREAL
                     AAELAAVEWHRYPDRAATELREAIAAHHGVSAAQVFVANGSNEVLQTLCLTYGGPGRS
                     VATFEPTYALHSHIPKITNTAVVSGERTEDFALDMDEVRRVLTEHDPTITFLCSPNNP
                     TGMVETEETVRAVADLAPGLVVVDEAYGQFAPWSALELVDETSPIVVTRTYSKTWSMA
                     AARLG"
BASE COUNT          260 a          834 c          666 g          253 t
ORIGIN      
        1 ttcgggaccg gatcgggcca ccggtagcct gcgccgggtg accggccccc tcaccacgct
       61 cgacctgcgc ggcaccgaca cgtcggatcc ccatgcgctg cggtcggtgc tgccccgccc
      121 gaaggcggcc gaggagccgc cgatcgagca ggtgcgcgcc gtcatcgagc gggtgcgcga
      181 ggggggcgac gacgcgctgc gggccatgac caaggagttc gacggggtcg acatcgacac
      241 gatccgggtc gaccccgacg acgtcgcccg ctccgtcgag cgcatcgacc cggcgctgcg
      301 caccgccctc gagaccgcgc acggcaacat cgccgcctac caccgggccc agctgcacga
      361 tcccgcccgc tacgagcgcg acggcctggt cgtgcgcgag ctgcgccgcc ccgtcgaccg
      421 ggccggcctc tacgtgccct ccgccctcgc cccgctgctg tccacggcgc tgatgaccgc
      481 cgtgcccgcc aaggtcgccg gcgtccacga gaccgtgctg tgcaccgcgc ccggtcccga
      541 cggtgcggtc gacgacggca tcctcgccgc ggccgccatc gccggcgtcg acgaggtcca
      601 ccgggtcgcc ggcccggctg ccatcgccgc cctcgcgtac ggcaccgagt ccattcggcc
      661 cgtcgacgtc atcgtcggtc ccggcagcgc tcgcgtcgcc caggccaagc gggaggtcgc
      721 gtccagcggc ctcgttggcg tgccgtccag cttcgccggt ccctccgagg tcgtggtcgt
      781 cgccgacgcc gccgccgtgg tggaggacgt cgccatcgac atcgtgctgc aggccgagca
      841 cggccccgac ggcctcgcct ggctgatctg ctgggacgac gaggtccgcg ccgccgtgga
      901 cgacgcggtg gcccggatcg tcgccgccag cccccgccgc gagcacatcg aggccaccct
      961 cgccgacgcc ggctacgccg tcgtggtcga cgggcccacc gccgccatcg acgtcgccaa
     1021 cgtcatcgcc cccgagcacc tcgagctgca gaccgccgac gccgaggacc tgctcgtccg
     1081 ggtcaagcac gccggcgccg tgttctgcgg gccgtgggcc ccggccagcg tcggcgacta
     1141 cgtggccggc cccaaccacg tgctgcccac ctacggctcg gcccgcttcg ccggggcgct
     1201 gcgcgtcgac gacttctgca agcacatcca cgtcgtcagc ctcgacgagg ccgcgctgcg
     1261 caccctcgcc ccccacgtcg aggcgatggc ggccgccgag ggcctcgcgg cccacgccga
     1321 ctccgtgcgc cagcgggtcg agcggtgaag cggcctcccg tccgcgacga cctcgccctc
     1381 caccccggct accactcccc gcaggtcgag gtcgacgtcc gcctcaacac caacgaggcc
     1441 cccgaggccc cgcccgaggg cttccgcgag gcgctcgccg ccgagctggc cgccgtcgag
     1501 tggcaccggt accccgatcg cgccgccacc gagctgcgcg aggccatcgc cgcgcaccac
     1561 ggcgtgtccg cggcgcaggt gttcgtggcc aacggctcca acgaggtgct gcagaccctg
     1621 tgcctcacct acggcgggcc gggccgcagc gtcgccacgt tcgagcccac gtacgccttg
     1681 cactcccaca tccccaagat caccaacacc gccgtggtgt cgggggagcg caccgaggac
     1741 ttcgctctcg acatggacga ggtccgccgg gtcctcaccg agcacgaccc gaccatcacg
     1801 ttcctgtgct cgcccaacaa ccccaccggg atggtcgaga ccgaggagac cgtccgcgcc
     1861 gtggccgacc tcgcgcccgg cctggtcgtg gtcgacgagg cctacgggca gttcgctccg
     1921 tggtcggcgc tggagctggt cgacgagacc tcgcccatcg tggtcacccg cacctactcc
     1981 aagacctggt ccatggccgc tgcccgcctc ggc
//
LOCUS       DATISU010000099         2862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_749816, whole genome shotgun sequence.
ACCESSION   DATISU010000099 DATISU010000000
VERSION     DATISU010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749816"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VLR27_04210"
     CDS             complement(<1..993)
                     /locus_tag="VLR27_04210"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HSP02678.1"
                     /translation="MAVTDRDAGIRTSMVPPPEVLPDVLPTDRLGPTGKPIREIRDGL
                     RRIDDVRNVGSVLLTWGHVALTIAFALYLDHPVAWVVSFVFMGAVHARMAILMHEAAH
                     KLLFTNKRVNDSVGRWLAAYPAFVPMELYRRSHFAHHREEFGPEEPDLNLYQGYPITR
                     ASLKRKLWRDLRGTSGWKSLKVLLQAVRKEQGFGIARKIVVAQVALAVVLTTWGVLVA
                     GAVGALTYPVLWLGSWLTVWRVVNRLRSIAEHGGMQASPDRRQTTHHVRQNLLARFWI
                     APFNTGWHLAHHVDMGVPFRNLPQMHRELVDAGWVTDGYVYPTYRALWRDLSSRP"
     gene            1020..1808
                     /locus_tag="VLR27_04215"
     CDS             1020..1808
                     /locus_tag="VLR27_04215"
                     /inference="COORDINATES: protein motif:HMM:NF019463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HSP02679.1"
                     /translation="MSDKRDIGGRPLTARSVIASTLLGVDPPRLPTLALVRSGELFGL
                     SEGATRTALSRMAAAGDVEADGGGYRLAGPLLERHARQQSARDARPRSWDGTWELAVV
                     PDGRRPAAERARLREAARRLRLAEVREGCWARPDNLDPARSPAERAIVDVQCLTLRGA
                     QPDDAGAFVGAFDPDGWATTARALLDAMVRAQPALDDRDAGVLADTFHLDAAVVRHLL
                     ADPALPVELEPPDWPARALRDAFARFDEGFKATWRAWYRTFAPD"
     gene            1853..2599
                     /locus_tag="VLR27_04220"
     CDS             1853..2599
                     /locus_tag="VLR27_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HSP02680.1"
                     /translation="METGGRSIRGLAALGVALALLLGACGGSDADVASDGERPTEQRD
                     RGRERDGDPDDTDDDEVAAATTSSTTTTTSTTTTTTTTTTAPPPTTTTQPPPPPTTAP
                     PPPPPPPTTAPPPPPPSGPRHGTRNTGCEQYMYNQINAARANAGRGALSFDTGIQYIA
                     VDWSDEMAGSQTLRHNPSYGDQIVRHRDYRTAGENVGRGYEQGSLFQAFMNSPGHRAN
                     IESTAFSHITVGCLTDGGGQLWVTQNFWGG"
     gene            2680..>2862
                     /locus_tag="VLR27_04225"
     CDS             2680..>2862
                     /locus_tag="VLR27_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSP02681.1"
                     /translation="MSTDRLYFRQLLAGRDFAQGDMIARQMVNFVYLVGDRETGEAVA
                     IDPAYDVQGLVDVLAED"
BASE COUNT          409 a         1075 c         1017 g          361 t
ORIGIN      
        1 ggggcggctg gagaggtccc gccagagtgc gcggtaggtg gggtacacgt agccgtcggt
       61 gacccagccg gcgtcgacca gctcccggtg catctggggc aggttgcgga acgggacgcc
      121 catgtcgacg tggtgggcca ggtgccagcc ggtgttgaac ggggcgatcc agaagcgggc
      181 cagcaggttc tggcgcacgt ggtgggtggt ctggcgccga tcgggcgacg cctgcatccc
      241 gccgtgctcg gcgatggagc gcaggcggtt gaccacccgc cacacggtga gccacgagcc
      301 gagccagagc accgggtagg tgagcgcacc gacggcgccg gcgaccagca cgccccacgt
      361 cgtcagcacg acggcgaggg ccacctgggc gaccacgatc ttgcgggcga tgccgaagcc
      421 ctgctccttg cgcaccgcct ggaggaggac cttcaggctc ttccaaccgg atgtgccccg
      481 caggtcccgc cacagcttgc gcttcagcga cgcccgggtg atcgggtagc cctggtacag
      541 gttgaggtcg ggctcctcgg ggccgaactc ctcccggtgg tgggcgaagt ggctgcggcg
      601 gtacagctcc atgggcacga aggccgggta ggcggccagc cagcggccga cggagtcgtt
      661 cacccgcttg ttggtgaaga ggagcttgtg ggcggcctcg tgcatgagga tcgccatgcg
      721 ggcgtgcacc gcgcccatga acacgaacga cacgacccag gcgacggggt ggtcgaggta
      781 gagggcgaag gcgatggtga gcgccacgtg accccacgtg agcagcaccg agccgacgtt
      841 gcggacgtcg tcgatccggc ggaggccgtc gcgaatctcc cggatcggct tgcccgtggg
      901 ccccaggcgg tcggtgggca ggacgtccgg cagcacctcg ggcgggggga ccatgctggt
      961 ccggatgcct gcgtcacgat cggtcacggc cacgccctca tcttacagtt gcgctgcgcg
     1021 tgtcggacaa gcgtgacatc ggcggccggc cgctcacggc gcggtcggtg atcgcgtcga
     1081 cgctgctcgg cgtcgacccg ccccgcctgc cgaccctcgc gctggtccgc tccggtgagc
     1141 tgttcgggct gtccgagggc gccacccgca ccgcgctgtc ccgcatggcc gccgccgggg
     1201 acgtggaggc cgacggtggc gggtaccggc tggccggtcc gctgctcgag cgccacgccc
     1261 gccagcagtc ggctcgcgac gcccgccccc ggtcgtggga cggcacctgg gagctcgccg
     1321 tggtccctga cgggcgacgg ccggcggccg agcgggcccg gctgcgcgag gcggcccgcc
     1381 ggctgcgatt ggccgaggtc cgcgaggggt gctgggcccg tccggacaac ctcgacccgg
     1441 cccggtcccc cgccgaacgg gccatcgtcg acgtccagtg cctgaccctg cgcggggcgc
     1501 agcccgacga cgccggcgcc ttcgtcggcg ccttcgatcc cgatggctgg gccaccaccg
     1561 cccgcgcgct cctcgacgcc atggtccggg cccagcccgc gctggacgac cgggacgccg
     1621 gggtcctggc cgacaccttc cacctcgatg ccgccgtggt gcggcacctg ctcgccgacc
     1681 ccgccctgcc cgtcgagctc gagccgccgg actggccggc gcgagcgctg cgggacgcct
     1741 tcgcccgctt cgacgagggg ttcaaggcga cctggcgggc ctggtaccgg acgttcgcgc
     1801 cggactgatg ccgtcgctca ggtccgagcc ggttcgtgcc gacaacgcag gcatggaaac
     1861 cggagggagg tcgatccgag gactcgccgc gctcggcgtc gcgctcgccc tcctcctcgg
     1921 cgcgtgcggc ggctccgacg ccgacgtggc ctccgacggc gagcgcccca ccgagcagcg
     1981 ggaccgcggg cgcgagcgcg acggggatcc cgacgacacc gacgacgacg aggtcgccgc
     2041 cgccaccacc agctccacga ccaccaccac gtcgaccacg accacgacca ccaccaccac
     2101 gacggcgccg ccgccgacga ccacgacgca gccgcccccg cccccgacga cggcaccgcc
     2161 gccccctccc ccgccgccca ccaccgcgcc accgccgccg cccccctccg ggccccggca
     2221 cggcacccgc aacaccggct gcgagcagta catgtacaac cagatcaacg ccgcccgggc
     2281 caacgccggc cggggcgcgc tgtccttcga caccggcatc cagtacatcg cggtcgactg
     2341 gtccgacgag atggccggca gccagaccct gcggcacaac ccgagctacg gggaccagat
     2401 cgtgcggcac cgggactacc gcaccgccgg cgagaacgtg gggcgcggct acgagcaggg
     2461 ctcgctgttc caggcgttca tgaactcacc cgggcaccgg gccaacatcg agtccaccgc
     2521 cttcagccac atcaccgtcg gctgcctcac cgacggcggc ggccagctct gggtcaccca
     2581 gaacttctgg ggcggctgac cgttctggcc tacatctgtg accaccagat ggcatcgatg
     2641 tagtccagaa cggttggtgg ccggccgtag gctcgaccca tgtcgacgga tcgcctctac
     2701 ttccgccagc tgctcgccgg acgcgacttc gcccaggggg acatgatcgc ccgccagatg
     2761 gtgaacttcg tctacctggt gggcgaccgg gagaccggcg aggcggtggc catcgacccg
     2821 gcctacgacg tgcagggcct cgtcgacgtc ctcgccgagg ac
//
LOCUS       DATISU010000100         3997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_4922, whole genome shotgun sequence.
ACCESSION   DATISU010000100 DATISU010000000
VERSION     DATISU010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4922"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1758)
                     /gene="glgB"
                     /locus_tag="VLR27_04230"
     CDS             complement(<1..1758)
                     /gene="glgB"
                     /locus_tag="VLR27_04230"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HSP02682.1"
                     /translation="MSIDPDLQRLVEGFHHDPHAVLGLHEGDDGPVVRIRRPEAKAAW
                     MDIDGTRCEGTHLVGGVFEIPVPREPKASEPVRVSYERFDVDVVDPYRFWPTLGDVDL
                     HLIGEGRHERLWAVLGARTMTHDGVDGVAFAVWAPAAKAVRVVGDWNSWDGRLHPMRA
                     LGASGVWELFVPEAEIGHRYRYEIVGADDQLRLKSDPLATATEMPPANASVITRSEHT
                     WSDDDWMAARRENDPINDRLSVYEVHAGSWRTVPEEGDRPLRWDELAEQLADHVIELG
                     FTHIELLPVAEHPYAPSWGYQVSGYFAPTSRFGSPDDFRAFVDRMHQRGVGVIVDWVP
                     AHFPKDDWSLGRFDGTALYEHADPRQGEHPDWGTYVFNFGRNEVRNFLVANALYWLEE
                     FHIDGLRVDAVASMLYLDYSRNDGEWLPNELGGRENLEAISFLQETNTVVHGAFPGVR
                     MIAEESTAWGGVSRPVHLGGLGFGQKWNMGWMHDTLEYFKADPVYRSHHHGQLTFGFV
                     YAWSENFVLPLSHDEVVHGKGSLLAKMPGDEWQRFANLRTLYGWMWGHPGKQLLFMGC
                     EFAQEREWSESRSLDWHLLD"
     gene            complement(1755..3089)
                     /locus_tag="VLR27_04235"
     CDS             complement(1755..3089)
                     /locus_tag="VLR27_04235"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02683.1"
                     /translation="MITAEEILPLVPDHLAAQRWYDHDGPPEAVELADSERWMDGDQD
                     LWWLLVDAMSGARSLGRYQLVIGARPANEEHPFLHGKSGELLGIVETDDGPATVYDAF
                     IDPPLALEVLHRIAPSLEASSVRALAVEQSNTSIVYDEHVIVKLFRRIHVGENPDVDA
                     VTRLAEHGFTHVPDQFGVLERPVQDGPDAGAVQHLAVARGFLTGASDGWHLALASLRV
                     VIADHTESAASGGDFGPDAQRLGAITGELHTRLAEAYGTSDADVSAWVGHFRAQLDRV
                     REHVPAEEISRRYDRAAEVRDPGPAVRIHGDLHLGQMLRADSGWYVIDFEGEPARPLA
                     ERLAPSSPLRDVAGMLRSFHYAAAVVHHERGEDEDAAELVAEWEERARSRFLEGYFAT
                     PGITELLPDEATTDLLLQAFELDKAVYEVGYETAYRPSWVGIPLGAIRRLLA"
     gene            complement(3086..>3997)
                     /locus_tag="VLR27_04240"
     CDS             complement(3086..>3997)
                     /locus_tag="VLR27_04240"
                     /inference="COORDINATES: protein motif:HMM:NF027968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HSP02684.1"
                     /translation="ECQMNFHFPLMPRMFMAVRREQRFPITEILAQTPEIPDNCQWGI
                     FLRNHDELTLEMVTDEERDYMYAEYAADPRMKINVGIRRRLFPLLDNDRRKAELFHAL
                     LYSMPGSPIMYYGDEIGMGDNIYLGDRDGVRTPMQWTPDRNAGFSKSDPAKLYLPTVM
                     DPVYGYSAVNVEAQQHDQSSFLHWVRRMLEVRREFPVFGTGRFEVLHAENPSVLAYLR
                     SSKEEDDDTSVLCVHNLSRFPQPCELMLGQHAGRIPIELTGRIPFPEIGELPYFITLP
                     PYGFYWFTLVDPETDGMGEEPYGRVEP"
BASE COUNT          576 a         1303 c         1507 g          611 t
ORIGIN      
        1 gtcgagcagg tgccagtcga ggctgcgcga ctcggaccac tcccgctcct gggcgaactc
       61 gcagcccatg aacaggagct gcttgcccgg gtgcccccac atccagccgt agagggtgcg
      121 caggttggcg aagcgctgcc actcgtcgcc gggcatcttg gccagcagcg agcccttccc
      181 gtggacgacc tcgtcgtggc tgaggggcag cacgaagttc tcgctccagg cgtacacgaa
      241 gccgaatgtg agctggccgt ggtggtgcga gcggtagacc gggtcggcct tgaagtactc
      301 gagcgtgtcg tgcatccagc ccatgttcca cttctggccg aagccgaggc ccccgaggtg
      361 cacgggccgg ctgacgccgc cccacgcggt ggactcctcg gcgatcatcc gcacgcccgg
      421 gaaggcgccg tgcacgaccg tgttggtctc ctgcaggaac gagatggcct cgaggttctc
      481 ccggccaccg agctcgttgg gcagccactc gccgtcgttg cgggagtagt cgaggtagag
      541 catcgaggcc acggcgtcga cccggaggcc gtcgatgtgg aactcctcga gccagtacag
      601 ggcgttggcg acgaggaagt tgcggacctc gttgcggccg aagttgaaca cgtaggtgcc
      661 ccagtcaggg tgctcgccct ggcgggggtc ggcgtgctcg tacagcgccg tcccgtcgaa
      721 gcgcccgagg gaccagtcgt ccttggggaa gtgggccggc acccagtcga cgatgacgcc
      781 gacgcctcgc tggtgcatgc ggtccacgaa ggcgcggaag tcgtcggggc tgccgaagcg
      841 cgacgtcggg gcgaagtacc cggacacctg gtagccccag ctgggggcgt aggggtgctc
      901 ggccacgggc agcagctcga tgtgggtgaa gcccagctcg atgacgtggt cggcgagctg
      961 ctcggccagc tcgtcccagc gcagcgggcg gtcgccctcc tcgggcacgg tgcgccacga
     1021 gccggcgtgg acctcgtaga cggagaggcg gtcgttgatc gggtcgttct cccggcgggc
     1081 cgccatccag tcgtcgtcgc tccacgtgtg ctcggagcgg gtgatgacgc tggcgttcgc
     1141 cggcggcatc tcggtggcgg tggccagcgg gtcggacttc aggcggagct ggtcgtcggc
     1201 gccgacgatc tcgtagcggt accggtggcc gatctcggcc tcgggcacga acagctccca
     1261 caccccggac gcgccgaggg cgcgcatggg gtggagccgg ccgtcccagg agttccagtc
     1321 gcccacgacg cgcacggcct tggccgccgg tgcccacacc gcgaacgcga cgccgtcgac
     1381 cccgtcgtgg gtcatggtgc gggcgcccag cacggcccag agccgctcgt ggcggccctc
     1441 gccgatgagg tgcaggtcga cgtcgcccag ggtgggccag aagcggtacg gatcgaccac
     1501 gtcgacgtcg aaccgctcgt aggagacccg gaccggttcg gacgccttgg gctcgcgcgg
     1561 cacggggatc tcgaacacgc cgccgacgag gtgggtgccc tcgcagcggg tgccgtcgat
     1621 gtccatccag gccgccttcg cctcggggcg gcggatgcgc accaccgggc cgtcgtcgcc
     1681 ctcgtgcagg ccgaggacgg cgtgggggtc gtggtggaag ccctccacca gtcgttgcag
     1741 gtcgggatcg atgctcatgc gagcaacctc cggatggcgc cgagtgggat gcccacccag
     1801 ctgggtcggt acgcggtctc gtagcccacc tcgtagacgg ccttgtccag ctcgaaggcc
     1861 tggaggagca ggtcggtggt ggcctcgtcg ggcaggagct cggtgatgcc gggtgtggcg
     1921 aagtagccct cgaggaaccg gctgcgcgcc cgctcctccc actcggccac cagctcggcg
     1981 gcgtcctcgt cctcgccccg ctcgtggtgg accacggcgg cggcgtagtg gaacgagcgc
     2041 agcatgccgg cgacgtcgcg cagcggcgac gagggtgcca ggcgctcggc caggggacgg
     2101 gcgggctcgc cctcgaagtc gatgacgtac cagccgctgt cggctcgcag catctggccg
     2161 aggtgcaggt cgccgtggat gcgcacggcc gggcccgggt cgcggacctc ggcagcccgg
     2221 tcgtagcgac gcgagatctc ctcggccggc acgtgctcgc gcacgcggtc gagctgggcg
     2281 cggaagtggc cgacccaggc gctcacgtcg gcgtcggagg tcccgtaggc ctcggcgagc
     2341 cgggtgtgga gctcgccggt gatggcgccg aggcgctggg cgtcggggcc gaagtcgcca
     2401 ccgctggccg cgctctcggt gtggtcggcg atgacgacgc gcaacgaggc caacgccagg
     2461 tgccagccgt cgctcgcacc ggtgaggaac ccgcgggcca cggcgaggtg ctggacggca
     2521 cccgcgtcgg gcccgtcctg caccggccgc tcgagcacgc cgaactggtc ggggacgtgg
     2581 gtgaagccgt gctcggccag ccgggtgacg gcgtcgacgt cggggttctc gccgacgtgg
     2641 atgcgccgga acagcttcac gatgacgtgc tcgtcgtaga cgatggaggt gttggactgc
     2701 tccacggcca gggcgcgcac cgatgaagct tcgagcgacg gcgcgatccg gtggagcacc
     2761 tcgagggcca gcggcgggtc gatgaaggcg tcgtagacgg tcgccgggcc gtcatcggtc
     2821 tcgacgatgc cgagcagctc gccgctcttg ccgtgcagga acgggtgctc ctcgttcgct
     2881 gggcgggcgc cgatcacgag ctggtagcgg ccgagggagc gcgcgccgga catggcgtcg
     2941 accagcaacc accagaggtc ctggtcgccg tccatccagc gctcgctgtc ggccagctcg
     3001 accgcctcgg gtggtccgtc gtggtcgtac cagcgctggg cggcgaggtg gtccggcacc
     3061 agtgggagga tctcttcagc agtgatcatg gctcgacccg tccgtagggc tcctcgccca
     3121 tcccgtcggt ctccgggtcc acgagggtga accagtagaa gccgtagggc gggagcgtga
     3181 tgaagtaggg cagctcgccg atctcgggga acgggatgcg accggtgagc tcgatgggga
     3241 tgcgtcccgc gtgctggccg agcatgagct cgcacggctg cgggaagcgc gacaggttgt
     3301 ggacgcacag caccgaggtg tcgtcgtcct cctccttgct ggagcgcagg tacgccagga
     3361 ccgaggggtt ctcggcgtgc aggacctcga agcgtccggt gccgaagacc gggaactccc
     3421 ggcgcacctc cagcatgcga cgcacccagt gcaggaacga cgactggtcg tgctgctggg
     3481 cctcgacgtt caccgccgag tagccgtaga cggggtccat cacggtgggc aggtacaact
     3541 tggccggatc ggacttggag aatccggcgt tgcggtccgg cgtccactgc atcggcgtgc
     3601 ggacgccgtc gcggtcaccg aggtagatgt tgtcgcccat cccgatctcg tcgccgtagt
     3661 acatgatcgg gctgcccggc atggagtaca gcaaggcgtg gaacagctcg gccttgcgcc
     3721 ggtcgttgtc gagcagcggg aagagccggc ggcggatgcc gacgttgatc ttcatgcggg
     3781 ggtccgccgc gtactcggcg tacatgtagt cccgctcctc gtcggtgacc atctcgagcg
     3841 tgagctcgtc gtggttgcgc aggaagatgc cccactggca gttgtccggg atctcggggg
     3901 tctgggccag gatctcggtg atggggaagc gctgctcccg gcgcaccgcc atgaacatgc
     3961 ggggcatcag ggggaagtgg aagttcatct ggcactc
//
LOCUS       DATISU010000101         8447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_564013, whole genome shotgun sequence.
ACCESSION   DATISU010000101 DATISU010000000
VERSION     DATISU010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8447
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564013"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VLR27_04245"
     CDS             complement(<1..212)
                     /locus_tag="VLR27_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HSP02685.1"
                     /translation="MAAPAGEAPEYELVGDLLRKALPVAPVAVAVAAVFAGIDGALSA
                     GVGVALVAGNLLLAAASLVWAGRISLG"
     gene            complement(218..538)
                     /locus_tag="VLR27_04250"
     CDS             complement(218..538)
                     /locus_tag="VLR27_04250"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HSP02686.1"
                     /translation="MSPRSDKPALNELLGDESARGIELAVTPVVFGGFGWLVDGWLGT
                     GPWLTLGLAAFALVGTFAKLWFTYDAEMRELESTSRWARRDGEPAAAPADDVDLWSDR
                     RAQA"
     gene            complement(659..1777)
                     /locus_tag="VLR27_04255"
     CDS             complement(659..1777)
                     /locus_tag="VLR27_04255"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HSP02687.1"
                     /translation="MDALLVFAAAALTTLVLTPLAGRLAVRLGAIAVPDDRRVHARPT
                     PTLGGLGILAGFLVGMLVGWRGSGFGGIFTAPTVPLGVVIAAVIVYAVGAVDDVREIS
                     APAKTAGTVLAGSILNIAGVSILLFRMPEIGLIDLSPDLAALVTVLWVVGMCQAINLI
                     DGLDGLAAGIVAIASGALFLFADKLGDPSVAQLPADNVAPLVALATCGACVGFLPHNW
                     NPASQFMGDGGALLLGLLMAASSIAVGGNSLADSSSQTFFFFAPLFIPLVILGVPIFD
                     TAYAIVRRVRKGMSFAEADKGHLHHRLMDLGHGQRRSVAILWLWTALLSGLVLYPVYT
                     DSDDGALPIGIAALALLLYTVLHPRARTERRARREPQP"
     gene            complement(1814..3058)
                     /gene="glyA"
                     /locus_tag="VLR27_04260"
     CDS             complement(1814..3058)
                     /gene="glyA"
                     /locus_tag="VLR27_04260"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HSP02688.1"
                     /translation="MGWPEIDDDELFGLIDREVERQNTTIQLIASENFTSPAVMRATG
                     SVLTNKYSEGYPGKRYYGGNEVIDEVETLAIERAKSLFGAAHANVQPHAGANANMGVY
                     LALLDPGDTVLGLRLDQGGHLTHGSPVNASGKLYDFVSYGVTPSDERIDFDQVRDVAL
                     EHRPKIIVAGATAYPRIIDPQPFRDIADEIGAYFMFDAAHIAGLIAGGVHPSPVPVAD
                     IVTFTTHKTLRGPRGGTILCTEELAPTIDKAMFPGLQGGPLDHVIAGKAVAFAEAATE
                     EFRDYAAQIVRNAEALAKGLAGRGLRLVSGGTDNHLLLVDLRSYDEDLSGKKARLALD
                     RAGISLNENTVPDDPRPPYITSGLRIGTPAVTTQGMGEDEMDVIAGLIVRTLDGREDE
                     SEIAAVRDEVRALCGKFEPYPV"
     gene            complement(3105..3563)
                     /gene="rpiB"
                     /locus_tag="VLR27_04265"
     CDS             complement(3105..3563)
                     /gene="rpiB"
                     /locus_tag="VLR27_04265"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HSP02689.1"
                     /translation="MRIAIGADHAGYDLKQHLLATIADLGHEAIDMGTDSTDSVDYPP
                     IMAAVGQAVASGDADRGIVLGGSGQGEQIAANKVRGVRAALCNDLYTARMSREHNDAN
                     VLAMGARIVAPGLAEEILQLWLTTPFEGGRHERRVDGIHAIENGNGGTAG"
     gene            complement(3587..4177)
                     /locus_tag="VLR27_04270"
     CDS             complement(3587..4177)
                     /locus_tag="VLR27_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02690.1"
                     /translation="MPHRVTGSLRRIDPSAVRILVVCTGNICRSPMAEAMLRQRLDER
                     GVPAEITSAGFVTEDRPASPPAVELLSGRGMDLRAHRSQRVTVDHLGDPDLIVCMERM
                     HVREIAVLDRDAFERTFTLPDLARRAEAHGPRRADEGVRDWLRRTGADRRPTDVLGVA
                     PEDEVPDPYGRPAAAYRRTAETLDELLDVVVSHLFP"
     gene            4234..5721
                     /locus_tag="VLR27_04275"
     CDS             4234..5721
                     /locus_tag="VLR27_04275"
                     /inference="COORDINATES: protein motif:HMM:NF036848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HSP02691.1"
                     /translation="MSLDPRTPVIIGVGQRSQRVDRGEPELEPVDLMVVAGQAALEDA
                     SVTDASAIDSVRTVGLLSWRYTDPGSLVAERLGVGHAVDSGISAMGGNSPQMLLNQTA
                     LDIQAGEVSCVLLAGAESWRTRSKYRAAGENPPWTTEPEGTEPAREIGTAVEMNHEAE
                     TALGIFLPIQVYPMFEQARRHHLGRTMDEHLPILGGLWSRFSEVAAKNPHAWIQEAMT
                     PEEIVTPSPTNRMVGMPYTKVMNSNNAVEQSAAVLVCSVEVAERLGVPRDRWVFPLAG
                     SDAHEHPYVSHRDTLYTAPAVGIAGRAALAEAGLTIDDIDHVDLYSCFPVAVQVGADE
                     LGLDLDRQLTLTGGLSFAGVPWNNYVMHSIAAMVDALRGDPGSTGLVWANGGYLTKHA
                     FGIYSTEPPAEGFRHAHPQDEIDALPSREVVADHDGEATVETWTVMHGKDGDPEQGIV
                     AMLLDDGRRAWGTTDDADALATMVAEDIIGRRVRRTAEGRVSLLG"
     gene            complement(5801..6241)
                     /locus_tag="VLR27_04280"
     CDS             complement(5801..6241)
                     /locus_tag="VLR27_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HSP02692.1"
                     /translation="MPDIDLSTISFFSGFDRDALDKIAAMGEAVEAERGAVIMEQGEV
                     GREAFVVVDGEVQIIVNGHVVGTAGRGSVLGEMALLDLRPRSATLVALTDVELISYAS
                     KQFRKILDEMPADARAALNERDERFRAENLEIGFDDDRPRPPGH"
     gene            complement(6279..6896)
                     /locus_tag="VLR27_04285"
     CDS             complement(6279..6896)
                     /locus_tag="VLR27_04285"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HSP02693.1"
                     /translation="MTAPVIPADDPGAVERAAKSLLSGGLIVLPTETVYGLAALASDP
                     DATRALFARKGRGADVPVAVLCADVEQAMALTSAVPPVARALAAAHWPGPLTMVLPRR
                     PGLGWALGEPTDTIGLRCPDHDLVRAIAARVGPLATTSANRHGLPTPGSATEAAEQLL
                     GPIDLVLDGGALTGVPSTVVDLTGDVPRVLRQGGVVIDAAGAPDA"
     gene            complement(6893..7750)
                     /gene="prmC"
                     /locus_tag="VLR27_04290"
     CDS             complement(6893..7750)
                     /gene="prmC"
                     /locus_tag="VLR27_04290"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HSP02694.1"
                     /translation="MVADTDTTSWAELHQQTVERLRAAGVENASNEARWIVERAAGDP
                     YRLVADTPVTVRSHHHWRLMVERREAGEPLQYVLERWPFRQLELFVDRRVLIPRPETE
                     VVVDAVLAEHARVGGPVVDLGTGSGAIALSVAAERPGTEVWGVERSPDALAVARANCT
                     GLGRKARTVRLVEGSWFAGLPDELRGEVGVVASNPPYVPDVAELPEDVERWEPAEALR
                     AGDDGLADIRVIVAEATEWLAPGGALVVEHGDDQGPSTRALAASAGFTEIRTGTDLAG
                     RERYLVARR"
     gene            7880..8407
                     /locus_tag="VLR27_04295"
     CDS             7880..8407
                     /locus_tag="VLR27_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP02695.1"
                     /translation="MSSEALDPAVVVLHGDGPSFDDLYRREHRPLLRLAWTLTGRRDL
                     AEELVQDAMLAVHRDWDRVRRYESPGGFARRVLLNAATSAARRRAAEGRALARMPTDD
                     VHRDELPPDDSFWSALRLLPERQAHAVALHYLEDLPVSEISRVLDIAEGTVKVHLHRG
                     RLALAELLRDELEEA"
BASE COUNT         1174 a         2925 c         3230 g         1118 t
ORIGIN      
        1 ccgaggctga tgcggccggc ccagacgagg ctggcggcgg cgagcaggag gttgccggcg
       61 acgagggcga cgcccacgcc ggcggagagg gcgccgtcga tgccggcgaa cacggccgcc
      121 acggccaccg ccaccggggc cacggggagg gccttgcgca gcaggtcgcc cacgagctcg
      181 tactcggggg cctcgccggc gggggcggcc acgggcatca tgcctgggcc cgccgatcgg
      241 accagaggtc gacgtcgtcg gccggagcgg cggccggctc accgtcgcgg cgggcccagc
      301 ggctggtgga ctcgagctcg cgcatctcgg cgtcgtaggt gaaccacagc ttggcgaagg
      361 tgcccacgag ggcgaaggcg gcgaggccga gcgtgagcca gggaccggtg ccgagccaac
      421 cgtcgacgag ccagccgaac ccaccgaaga ccacgggggt cacggcgagc tcgatgccac
      481 gggccgactc gtcaccgagc agctcgttca gggctggctt gtcgcttctc ggggacacgg
      541 ttcgagggga tccttcggcg ccgcttgtga atcgtttcgc aatctatgaa agcaggtcgc
      601 gagacggcaa atccagggtc ccccgacggc ccggggcaac gtagcaaagg ggggtggctc
      661 agggctgcgg ctcgcggcgg gcccggcgct cggtgcgggc ccgggggtgc agcacggtgt
      721 agagcagcag cgcgagggcg gcgatgccga tggggagggc accgtcgtcg gagtcggtgt
      781 acacggggta cagcaccagg cccgacagca gcgcggtcca gagccagagg atggccacgc
      841 tgcgccgctg gccgtgcccg aggtccatga ggcggtggtg gaggtggccc ttgtcggcct
      901 cggcgaagct catgcccttg cgcacccgtc ggacgatggc gtaggcggtg tcgaagatcg
      961 gcaccccgag gatcaccagc gggatgaaca gcggggcgaa gaagaagaac gtctggctcg
     1021 aggagtcggc cagggagttg ccgccgacgg cgatggacga ggccgccatg agcaggccga
     1081 gcaggagcgc gccgccgtcg cccatgaact gcgacgccgg gttccagttg tggggcagga
     1141 accccacgca ggcgccgcac gtggccaggg cgacgagcgg cgccacgttg tcggcgggca
     1201 gctgggcgac ggaggggtca cccagcttgt cggcgaacag gaacagcgca ccggaggcga
     1261 tggccacgat gccggcggcg aggccgtcga ggccgtcgat gaggttgatc gcctggcaca
     1321 tgccgaccac ccagagcacg gtgacgaggg cggcgaggtc cggggacagg tcgatgaggc
     1381 cgatctcggg catgcggaac agcaggatcg acacgccggc gatgttgagg atggatccgg
     1441 ccagcacggt gccggcggtc ttggccggcg ccgagatctc ccgcacgtcg tcgacggcgc
     1501 cgacggcgta gacgatgacc gccgcgatca cgaccccgag ggggacggtg ggggcggtga
     1561 agatgccgcc gaaccccgac ccgcgccagc cgaccagcat cccgacgagg aacccggcga
     1621 ggatgccgag gccgccgagc gtgggggtgg gacgggcgtg cacccgccgg tcgtcgggga
     1681 cggcgatcgc cccgagccgc accgcgagcc gacccgccag cggggtgagc accagcgtgg
     1741 tgagggcagc ggcggcgaag accaggagcg cgtccatggt gaggggtgcg ccggcacggg
     1801 ccggcgccgg cggtcagacc gggtagggct cgaacttgcc gcagagggcc cggacctcgt
     1861 cgcggacagc ggcgatctcg gactcgtcct cccggccgtc gagcgtgcgc acgatcagac
     1921 cggcgatgac gtccatctcg tcctcgccca tgccctgggt ggtgaccgcg ggcgtgccga
     1981 tgcgcaggcc ggaggtgatg tagggcgggc gggggtcgtc ggggacggtg ttctcgttga
     2041 gcgagatgcc ggcccggtcg agggcgaggc gggccttctt gccggacagg tcctcgtcgt
     2101 aggagcgcag gtcgacgagc aggaggtggt tgtcggtgcc gccggacacg agccgcaggc
     2161 cccggccggc gaggcccttg gccagcgcct cggcgttgcg cacgatctgg gcggcgtagt
     2221 cccggaactc ctcggtggcc gcctcggcga aggccacggc cttgccggcg atgacgtggt
     2281 cgagggggcc gccctgcagg ccggggaaca tggccttgtc gatggtcggg gccagctcct
     2341 cggtgcacag gatcgtgccg ccgcgagggc cacgcagggt cttgtgcgtg gtgaaggtga
     2401 cgatgtcggc caccgggacc ggtgaggggt gcacgccgcc ggcgatcagg ccggcgatgt
     2461 gggcggcgtc gaacatgaag taggcgccga tctcgtcggc gatgtcccgg aagggctgcg
     2521 ggtcgatgat ccgggggtag gcggtggccc cggcgacgat gatcttgggc cggtgctcga
     2581 gggcgacgtc gcgcacctgg tcgaagtcga tgcgctcgtc ggagggcgtg acgccgtagg
     2641 acacgaagtc gtagagcttg ccgctggcgt tcaccggcga gccgtgggtg aggtggccgc
     2701 cctggtcgag gcgcaggccg agcacggtgt cgcccgggtc gagcagggcc aggtacacgc
     2761 ccatgttggc gttggcgccg gcgtggggct gcacgttggc gtgggcggcg ccgaacagcg
     2821 acttggcccg ctcgatggcg agggtctcga cctcgtcgat gacctcgttg ccgccgtagt
     2881 agcgcttgcc ggggtagccc tcggagtact tgttggtgag caccgagccc gtggcccgca
     2941 tcacggccgg agaggtgaag ttctccgacg cgatcagctg gatggtggtg ttctggcgct
     3001 cgacctcccg gtcgatcagg ccgaacagct cgtcgtcatc gatctcgggc caacccatgg
     3061 tcccatcgtg ccaccgatgt cggtggcggt cgtgatcgcc ggcgtcagcc ggcggtcccc
     3121 ccgttgccgt tctcgatggc gtggatgccg tcgacccgcc gctcgtggcg gccgccctcg
     3181 aatggcgtgg tgagccacag ctgcaggatc tcctcggcca ggccgggcgc cacgatgcgg
     3241 gcacccatgg cgagcacgtt ggcatcgttg tgctcgcggc tcatccgggc cgtgtacagg
     3301 tcgttgcaca gggcggcgcg cacgccgcgc accttgttgg ccgcgatctg ctcgccctgg
     3361 cccgagccgc cgagcacgat gccccggtcg gcgtcgccgc tcgcgaccgc ctggcccacg
     3421 gcggccatga tcggcgggta gtcgaccgag tcggtcgagt cggtgcccat gtcgatggcc
     3481 tcgtgtccga ggtcggcgat ggtggcgagc aggtgctgct tgaggtcgta gccggcgtgg
     3541 tcggcgccga tggcgatgcg caatgggggc tccgggtcga ggtgggtcag gggaacaggt
     3601 gggagacgac gacgtcgagg agctcgtcga gggtctcggc ggtgcgacgg tacgcggcgg
     3661 cggggcggcc gtagggatcc ggcacctcgt cctcgggggc gacgcccagc acgtcggtcg
     3721 gccggcgatc cgccccggtg cgccggagcc agtcgcgcac accctcgtcc gcccgacggg
     3781 ggccgtgagc ctcggcccgg cgagcgaggt ccggcagggt gaacgtgcgc tcgaaggcgt
     3841 cccggtcgag gacggcgatc tcgcggacgt gcatccgctc catgcacacg atcaggtcgg
     3901 ggtcgccgag gtggtcgacg gtgacccgct ggctccggtg cgcccgcagg tccatgcccc
     3961 gcccggagag cagctccacc gcgggtggcg acgccggacg gtcctcggtg acgaagcccg
     4021 ccgaggtgat ctcggccggc accccgcgct cgtcgaggcg ctggcgcagc atggcctcgg
     4081 ccatgggcga gcggcagatg ttgccggtgc agacgacgag gatgcggacg gcggaggggt
     4141 cgatgcgccg gaggctaccg gtgacccgat gtggcatctg cggtcgaggc tcctccatac
     4201 ttgaccgttc ggtcaagtct ttcggaggta cccgtgtcgc tcgatccccg caccccggtc
     4261 atcatcggcg tcggccagcg gagccagcgc gtcgaccgcg gtgagcccga gctcgagccg
     4321 gtggacctca tggtcgttgc cggccaggcc gcgctcgagg acgcgtcggt caccgacgcc
     4381 tccgccatcg actcggtccg caccgtcggc ctgctgtcgt ggcgctacac cgatcccggc
     4441 tcgctcgtgg ccgagcggct cggcgtcggc catgccgtcg acagcggcat ctcggccatg
     4501 ggcggcaact ccccacagat gctgctgaac cagacggcgc tcgacatcca ggcaggggag
     4561 gtctcctgcg tcctgctggc cggagcggag tcgtggcgga cccgatcgaa gtaccgggcc
     4621 gccggcgaga acccgccgtg gaccaccgag cccgagggca ccgagccggc tcgggagatc
     4681 ggcaccgcgg tcgagatgaa ccacgaggcc gagaccgccc tcggcatctt cctgccgatc
     4741 caggtgtacc cgatgttcga gcaggcccgc cgccaccacc tcggccggac gatggacgag
     4801 cacctgccga tcctcggtgg cctgtggtcg cgcttctccg aggtggcggc gaagaacccg
     4861 cacgcctgga tccaggaggc gatgaccccc gaggagatcg tcaccccctc ccccaccaac
     4921 cgcatggtgg gcatgccgta cacgaaggtc atgaactcga acaacgccgt cgagcagtcg
     4981 gctgcggtgc tcgtgtgctc ggtcgaggtg gccgagcgcc tcggcgtgcc ccgcgaccgc
     5041 tgggtcttcc cgctggccgg ttccgacgcc cacgagcacc cctacgtctc gcaccgcgac
     5101 acgctctaca cggcgccggc cgtggggatc gccgggcggg ctgcgctcgc cgaggccggc
     5161 ctcacgatcg acgacatcga ccacgtcgac ctctactcgt gcttccccgt cgccgtgcag
     5221 gtcggcgccg acgagctcgg cctcgacctc gaccgccagc tgaccctcac cggcgggctg
     5281 agcttcgccg gcgtgccgtg gaacaactac gtgatgcact ccatcgccgc catggtggac
     5341 gcgctccggg gcgacccggg cagcaccggg ttggtgtggg ccaacggcgg ctacctcacc
     5401 aagcacgcct tcggcatcta ctccaccgaa ccgcccgccg agggcttccg ccacgcccac
     5461 ccccaggacg agatcgacgc cctgccctcc cgcgaggtcg tggccgacca cgacggcgag
     5521 gccaccgtcg agacctggac ggtgatgcac ggcaaggacg gcgaccccga gcagggcatc
     5581 gtcgccatgc tgctcgacga cggccgccgg gcctggggca ccaccgacga cgccgacgcc
     5641 ctcgccacca tggtcgccga ggacatcatc gggcgccgcg tgcgccgcac cgccgagggc
     5701 cgggtcagcc tcctcggctg acgccgcgac agatctcgtt ctggcctaca tcggtgccac
     5761 gtggtggtca tggatgtagg ccagaagcgg gtgctcgggg tcagtggccg gggggccggg
     5821 gccgatcgtc gtcgaagccg atctcgaggt tctcggcccg gaagcgctcg tcgcgctcgt
     5881 tgagggcggc ccgggcgtcg gccggcatct cgtcgaggat cttgcggaac tgcttcgacg
     5941 cgtagctgat gagctcgacg tcggtgagcg ccacgagcgt ggccgagcgg gggcgcaggt
     6001 cgagcagggc catctcaccg aggaccgagc cgcgaccggc cgtgccgacc acgtggccgt
     6061 tgacgatgat ctgcacctcg ccgtcgacga cgacgaacgc ctcgcggccg acctcgccct
     6121 gctccatgat caccgcaccg cgctcggctt cgacggcttc gcccatggcc gcgatcttgt
     6181 cgagcgcgtc acggtcgaag ccggagaaga aggagatggt ggagagatcg atgtcaggca
     6241 tgggtgcggc tccctcgagg tgtggtcgga gccggaggct aggcgtccgg agcaccggcc
     6301 gcgtcgatga ccacgccacc ctgacgcagc acccggggga cgtcgcccgt gaggtcgacg
     6361 accgtggacg gcacgccggt gagcgcaccg ccgtcgagca ccaggtcgat gggacccagc
     6421 agctgctcgg ccgcctccgt ggcggacccc ggggtgggca ggccgtggcg gttggcgctg
     6481 gtcgtggcca gcgggccgac ccgtgcggcg atggcccgga ccaggtcgtg gtcggggcag
     6541 cgcaggccga tggtgtccgt cggctccccc agcgcccagc cgagccccgg ccggcggggc
     6601 aggaccatcg tgagcgggcc gggccagtgg gcggcggcga gcgcgcgggc gaccgggggg
     6661 acggcgctgg tcagcgccat ggcctgctcg acgtcggcgc acagcacggc gacgggcacg
     6721 tcggcgcccc gccccttgcg ggcgaagagg gcgcgggtcg cgtccgggtc cgaggccagc
     6781 gccgccaggc cgtagacggt ctccgtgggc agcacgatga ggccgccgct cagcagggac
     6841 ttggcggcgc gctcgacggc accggggtcg tccgccggga tgaccggcgc ggtcaccgcc
     6901 gggccaccag gtagcgctcc cggccggcga ggtcggtgcc ggtgcggatc tcggtgaagc
     6961 cggcgctggc cgccagcgcg cgggtggacg gcccctggtc gtcgccgtgc tcgacgacca
     7021 gcgccccacc gggcgcgagc cactcggtcg cctcggccac gatcacccgg atgtcggcca
     7081 gcccgtcgtc gccggcgcgc agggcctccg ccggctccca ccgctcgacg tcctcgggca
     7141 gctcggccac gtcgggcacg tagggcgggt tcgacgccac gacgccgacc tcgccccgca
     7201 gctcgtcggg caggccggcg aaccacgagc cctcgaccag gcgcacggtg cgggccttgc
     7261 gaccgaggcc ggtgcagttg gctcgggcga cggcgagggc gtccggcgac cgctcgacgc
     7321 cccacacctc ggtgcccgga cgctcggcgg ccacggacag ggcgatggca cccgacccgg
     7381 tgccgaggtc cacgaccggg ccgcccaccc gggcgtgctc ggccaggacg gcgtcgacga
     7441 cgacctcggt ctcgggacgc gggatcagca cccggcggtc gacgaacagc tcgagctggc
     7501 ggaacggcca gcgctccagg acgtactgca gcggctcgcc cgcctcgcgc cgctcgacca
     7561 tgaggcgcca gtggtggtgc gagcgcaccg tgaccggcgt gtctgccacc aggcggtagg
     7621 gatcgccggc ggcccgctcg acgatccagc gggcctcgtt cgacgcgttc tcgacaccgg
     7681 cggcgcgcag gcgctcgacc gtctgctggt gcagctctgc ccagctcgtc gtgtcggtgt
     7741 cggcgaccat ggagccatcg atcctacgag caccccctcc cgttctggca gtgcccgtgt
     7801 tgcggccagg taaacctcag gaccgccctc gtgcgtgtgc tggggtgtgg atgcagtcgg
     7861 ggggatcgga ggtgcggcgg tgagcagcga ggcgctggac ccggccgtgg tcgtcctcca
     7921 cggagacggc ccgtccttcg acgatctcta ccgccgcgag caccgcccgc tgctccgcct
     7981 cgcctggacc ctgaccggcc gacgcgacct cgccgaggag ctcgtccagg acgccatgct
     8041 cgccgtgcac cgggactggg accgggtgcg gcgctacgag tcccccggcg gcttcgctcg
     8101 tcgggtgctg ctcaacgcgg cgacctcggc cgcccgccgg cgtgcggcgg agggccgggc
     8161 cctggcccgc atgcccaccg atgacgtcca ccgcgacgag ctgccgccgg acgactcgtt
     8221 ctggtcggcg ctgcgcttgc tccccgagcg ccaggcccac gccgtggcgc tgcactacct
     8281 ggaggacctc ccggtgtccg agatctcccg ggtgctcgac atcgccgagg gcaccgtcaa
     8341 ggtccacctg caccggggcc ggctggcgct cgccgagctg ctccgcgacg agctggagga
     8401 ggcgtgatgg aggacaccga gctgctcgag gcgctggacc gccgggc
//
LOCUS       DATISU010000102         3138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_377782, whole genome shotgun sequence.
ACCESSION   DATISU010000102 DATISU010000000
VERSION     DATISU010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377782"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..264
                     /locus_tag="VLR27_04300"
     CDS             49..264
                     /locus_tag="VLR27_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02696.1"
                     /translation="MSDRLVRIEEHRWLGDKRSFVVHDQDNVCDKTVLDDIAAAESWL
                     TFGPDTSVEARNRGYKVCPACDAKSTD"
     gene            282..1049
                     /locus_tag="VLR27_04305"
     CDS             282..1049
                     /locus_tag="VLR27_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02697.1"
                     /translation="MEEHFSSGSLTLVGHLAGQVDSGPPRPAVVIVHGYPSGPAGTAG
                     AVSAMAELADRIADDMGFVAFSPCLRGMSGSQGAFSIKGWKNDIHAAVDHVRSTARVS
                     GVWLIGFGTGGALAICAGADDTDIQGVAALAAPADFDDWATNPRQLHEHAREVGIVHA
                     DATAGLLDGWSREMRLTKAIDGAQRLRPRPLLVVHGSADDLVPDFDARVIADAHTAAE
                     LRLLDAAGHRLRHDPRAMAILLGWLDRQRNSAVPRTA"
     gene            complement(1046..1624)
                     /locus_tag="VLR27_04310"
     CDS             complement(1046..1624)
                     /locus_tag="VLR27_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HSP02698.1"
                     /translation="MVTPQIPITGNPDADQLLVDDPLALLLGMLLDQQVPMEWAFTGP
                     FTLRERLGHLDPHRIAAMPVDEFVDVCREKPAIHRFPKSMGERIHGACEHVVETYDGH
                     ADRIWTEAADAKDLYARLREVPGYGEEKTKIFIAILAKRLGVRLDGWEEAARPFSDDT
                     PRSVADVASEESLLEVRKWKKAQKAKKKSKAD"
     gene            1650..2858
                     /locus_tag="VLR27_04315"
     CDS             1650..2858
                     /locus_tag="VLR27_04315"
                     /inference="COORDINATES: protein motif:HMM:NF017135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HSP02699.1"
                     /translation="MAGDLSDDAWQDLYEGIERVLPVRDTSTQRQELARLARVYRRLD
                     DDGRAGFLRRAATHLGVDDDAARRAAEVLLEDPADPAAQVALRRALTPRFSDLLHLLT
                     GLDGGVKLLVDLRADLRHLARGELPMVEAELDGHLATVFDVGLLQLERITWGSSARLL
                     EKLIQYEAVHEMDGWEDLRDRLDADRRCFAFLHPAMPDEPVVFVEIALVTGPPTSLAE
                     LLDRNAPTCDAEDADTAVFYSITSCQRGLAGIHLGTELIKTVVRELQRELPHLERFVT
                     LSPIPTLRAADLDLSATTRAELEEGSWIAGDDADVRDEVLAATARYLSTRVDGRVPDP
                     VANFHLSNGALVDAVHWRANTEDYGLARSWGVMASYRYDPPTIAANAASYAAGGEVAV
                     SDDVAALRRI"
     gene            complement(2913..>3138)
                     /locus_tag="VLR27_04320"
     CDS             complement(2913..>3138)
                     /locus_tag="VLR27_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02700.1"
                     /translation="ARWVGRSYTSLGTDGFGRSDTREALRSFFEVDAPHVVVAVLASL
                     CARGEVEASTVAEAIEAHGIDPELTPPWTR"
BASE COUNT          412 a         1200 c         1094 g          432 t
ORIGIN      
        1 ggtgccggca ttgcccggcg cctccccgtc ccgcgtatcg tgcccggcat gtctgatcgc
       61 ctcgttcgga tcgaagagca ccggtggctg ggcgacaagc gctccttcgt cgtccacgac
      121 caggacaacg tctgcgacaa gaccgtgctc gacgacatcg ccgcagccga gtcgtggctg
      181 acgttcggtc ccgacacgag cgtcgaggcc cgcaaccgcg gctacaaggt ctgcccggcc
      241 tgcgacgcca agagcaccga ctgagcgccg ccgcccgccc cgtggaggag cacttcagct
      301 ccggctccct gaccctggtc ggccacctcg ccggccaggt cgactcgggc ccgccccggc
      361 ccgccgtcgt catcgtccac ggctacccgt cgggcccggc cggcaccgcc ggtgcggtct
      421 cggccatggc cgagctggcc gaccgcatcg ccgacgacat ggggttcgtc gccttctcgc
      481 cgtgcctgcg gggcatgtcg ggcagccagg gtgcgttctc gatcaagggg tggaagaacg
      541 acatccacgc cgcggtcgac cacgtccgct cgaccgcccg ggtcagcggc gtgtggctga
      601 tcgggttcgg caccggcggg gcgctggcga tctgcgccgg cgccgacgac accgacatcc
      661 agggggtcgc cgcgctggcc gcccccgccg acttcgacga ctgggccacc aacccccgcc
      721 agctccacga gcacgcgcgg gaggtcggca tcgtccacgc cgacgccacc gccgggctgc
      781 tcgacggctg gtcgcgcgag atgcgcctca ccaaggccat cgacggcgcc cagcggctgc
      841 ggccccggcc cctgctcgtg gtccacggct ccgccgacga cctggtgccg gacttcgacg
      901 cccgggtcat cgccgacgcc cacaccgccg ccgagctgcg gctgctcgat gcggccggcc
      961 accggctccg ccacgacccg cgcgccatgg ccatcctgct cggctggctc gaccgccagc
     1021 gcaactccgc ggtgccgcgg actgcctagt ccgccttgct cttcttcttg gccttctggg
     1081 ccttcttcca cttgcggacc tcgagcagcg actcctccga ggccacgtcg gccaccgagc
     1141 ggggcgtgtc gtcggagaac ggccgggccg cctcctccca cccgtcgagc cggacgccga
     1201 ggcgcttggc caggatggcg atgaagatct tcgtcttctc ctcgccgtac ccggggacct
     1261 cgcgcagccg ggcgtagagg tccttcgcgt cggcggcctc ggtccagatc cggtcggcgt
     1321 gcccgtcgta ggtctccacc acgtgctcgc acgcaccgtg gatgcgctcg cccatcgact
     1381 tggggaagcg gtggatggcg ggcttctccc ggcacacgtc cacgaactcg tcgacgggca
     1441 tggccgcgat gcggtgcggg tcgaggtggc cgaggcgctc ccgcagggtg aacggcccgg
     1501 tgaacgccca ctccatcggc acctgctggt cgagcagcat cccgagcagc agggccagcg
     1561 ggtcgtcgac gaggagctgg tcggcatcgg ggttgccggt gatcgggatc tggggcgtca
     1621 ccatgggccc gagcctacga tcccggtcca tggccgggga cctgagcgac gacgcctggc
     1681 aggacctgta cgagggcatc gagcgggtgc tgccggtgcg ggacacctcc acccagcgcc
     1741 aggagctggc ccgcctcgcc cgggtctacc gccgcctcga cgacgacggc cgcgccgggt
     1801 tcctgcgccg ggccgccacg cacctcggcg tcgacgacga tgccgcccgg cgcgccgccg
     1861 aggtgctgct cgaggacccg gccgatcccg cggcgcaggt cgcactccgc cgcgcgctga
     1921 cgccgcggtt ctccgacctg ctccacctgc tcaccggcct cgacggtggc gtgaagctgc
     1981 tcgtggacct gcgcgccgac ctgcggcacc tggcccgcgg cgagctcccg atggtggagg
     2041 ccgagctcga cgggcacctc gccaccgtgt tcgacgtggg cctcctgcag ctggagcgga
     2101 tcacctgggg gtcgtcggcc cgcctgctgg agaagctgat ccagtacgag gcggtgcacg
     2161 agatggacgg gtgggaggac ctgcgcgacc gcctcgacgc cgaccgccgc tgcttcgcct
     2221 tcctccaccc ggcgatgccc gacgagcccg tcgtcttcgt cgagatcgcc ctggtgaccg
     2281 gcccgccgac gagcctggct gagctgctcg accggaacgc acccacctgc gacgccgagg
     2341 acgccgacac cgccgtcttc tactccatca cctcgtgcca gcggggcctg gccggcatcc
     2401 acctcggcac cgagctgatc aagaccgtgg tgcgcgagct ccagcgggag ctgccccatc
     2461 tggagcgctt cgtgacgctc tccccgatcc cgacgctgcg cgccgccgac ctcgacctct
     2521 ccgcgaccac gcgcgccgag ctggaggagg gcagctggat cgccggtgac gacgccgacg
     2581 tccgggacga ggtgctggcc gccaccgccc gctacctgtc gacccgggtg gacggacggg
     2641 tgccggatcc cgtggcgaac ttccacctca gcaacggtgc cctcgtcgac gccgtgcact
     2701 ggcgggccaa caccgaggac tacgggctgg ctcgctcctg gggcgtgatg gccagctacc
     2761 gctacgaccc gccgacgatc gccgccaatg ccgcgtccta cgccgccggc ggggaggtcg
     2821 cggtcagcga cgacgtggcc gcgctgcgcc ggatctgagc gttctggtcc gcggcacacg
     2881 tctcggaggc gcgccccggg acccagaacg gttcagcggg tccagggcgg ggtgagctcc
     2941 gggtcgatgc cgtgggcctc gatggcctcg gcgacggtgg atgcctccac ctcgccccgg
     3001 gcgcagagcg aggcgagcac ggcgacgacg acatgggggg cgtcgacctc gaagaacgag
     3061 cgcagcgcct cccgggtgtc ggagcggccg aagccgtcgg tgccgagcga ggtgtaggag
     3121 cgaccgaccc accgggcc
//
LOCUS       DATISU010000103         9783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_98205, whole genome shotgun sequence.
ACCESSION   DATISU010000103 DATISU010000000
VERSION     DATISU010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9783
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98205"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VLR27_04325"
     CDS             complement(<1..692)
                     /locus_tag="VLR27_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02701.1"
                     /translation="MTDLLEPGVDPDVALKALEVAVGMVQAVDLDGLSPAEELALVGR
                     LEKLRRRLDHGTDLTVAHLDVGAAFSVDGHKTAKGALKAIGRLSGSDAHGRVQTSRAL
                     PHLPLVEAAYARGEIPTEHMRAIARTIANPRVAEWVPDADPIFAHHASVLGYDDFVAA
                     LRQWEALADADGADQAAERCHERRNASVQESAIDGSFRLESNHGALQGAAMAEIFDRF
                     ETAELHADWAAAR"
     gene            complement(890..1918)
                     /locus_tag="VLR27_04330"
     CDS             complement(890..1918)
                     /locus_tag="VLR27_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02702.1"
                     /translation="MSEDAVRWPSFCDLCGEYTTTPEHCSHCGHALHPDVPPEVPPPV
                     PAHPTTPFAEAAAGRRVEHRGLLAELAQWALAEGRRVDLDVAAVCIHALERQRTEDGI
                     HLDRRQVNWIMWGAVRNEVSPLRALLPDTWIEDLWTVLRFLVATERLTSDSDPEPALL
                     EPLQCYGGLDDDGRERPDGVDVDFFCQCHHPHDPTCPPGMVQVSLGHDWDDHFEYVGY
                     AHGIPRSVDVPMSAYEPLAKLARRHRAQPAMFNVALDQFDHLGTVPADGPVPKLWLYL
                     FTPARRRGWPPLALDEHGGAWRATRHRGRRRGFRWTSVDDRGAAHLCGLASWEFDHEH
                     RQRELLEQ"
     gene            complement(2065..2508)
                     /locus_tag="VLR27_04335"
     CDS             complement(2065..2508)
                     /locus_tag="VLR27_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP02703.1"
                     /translation="MPRNTHRNGVPADIFLERFKALVSALPSSRKHFAGWMRDADVDS
                     DVADDLEVVFSELTANAVAGSPAESDDIEVRAQIDDGMMVLDVSNLVEDPDRRPHTAT
                     DIGDPLRRGGRGLLITRSFMDSVEVQTEQMDRLVVHCSRRVAPAR"
     gene            2665..3417
                     /locus_tag="VLR27_04340"
     CDS             2665..3417
                     /locus_tag="VLR27_04340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP02704.1"
                     /translation="MTEDHFAGEVAARYDATLGPKGTPEELDPTVDLLAELVGDGAAL
                     ELAIGTGRVALPLAARGVAVAGIELSPDMVDQLRAKPGGADLDVVIGDMTTARVHGAF
                     RLVYLVYNTIGNVETQARQIACFANAAAHLEPGGCFVVEVGVPDLRRLVPGHDAVVFS
                     HAPGYVGYDRYVDLVAQRAVSHHFVADDSGAREVRTPFRYVWPSELDLMARLAGLRLR
                     HRWAGWDRGPFTDESTSHVSVWEKPADPGSVG"
     gene            3459..3791
                     /locus_tag="VLR27_04345"
     CDS             3459..3791
                     /locus_tag="VLR27_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HSP02705.1"
                     /translation="MSDPVEPDNDERRVALEQERDLLRSKVHELDSEGDDLSYDDNFA
                     DSGQVAAERGENKVLYDQLRRDLDDIEKALERMDSGTYGTCEVCGDTIGPDRLEVMPA
                     TRFCIQHA"
     gene            3799..4302
                     /locus_tag="VLR27_04350"
     CDS             3799..4302
                     /locus_tag="VLR27_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSP02706.1"
                     /translation="MAGPIHLVRRFLGSLSPVPLKAEDEAWTNAQLGPGERQLWQRLS
                     RADQKHAVGVARVVADRVGADERAVIAAALLHDIGKVEADLGTFGRVAATLVRRRRGA
                     RWKDAPGIRGRIGRYLHHDAIGAGLLADAGADPLTVAWAREHHRPEDAWTVPVAVGRV
                     LRDADDD"
     gene            4292..5248
                     /locus_tag="VLR27_04355"
     CDS             4292..5248
                     /locus_tag="VLR27_04355"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HSP02707.1"
                     /translation="MTTEPERLRSYTVVGAGAVGLLYGTRLAVAGHPVRWLVRSGADT
                     MAAEGIEVTSEGRTLRIDPAEVVVATEPGALPASDVVVVATKTTANDRLAELLDGAGV
                     PGATVAVFQNGLGAEEVVRDRVPEAGAVLGALCFVCAHRRGPGRAEHLDYGAVTLAPL
                     EPEHLAAADAVAADLQRAGVETAVLADLGVARWRKLVWNIPFNGLCTLLDASTDALLA
                     DTASRALVSALMDEVIAGARAVGHDIPEAFRDEMLAATDAMRPYDPSMKLDIAAGRPL
                     EIDAIYDVATRTARDAGAPMPRTEALAAMLRFRAGAVSSGST"
     gene            5266..5427
                     /locus_tag="VLR27_04360"
     CDS             5266..5427
                     /locus_tag="VLR27_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02708.1"
                     /translation="MDASWAISALLTLGFFLGAVMSTGALAAGWFVLAVGMAVITVVR
                     FRAAKRPPG"
     gene            complement(5424..6758)
                     /locus_tag="VLR27_04365"
     CDS             complement(5424..6758)
                     /locus_tag="VLR27_04365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSP02709.1"
                     /translation="MDADQILRAVRPLVLVVDGDGTVADVRGGYGGFFGHDVSQWVGA
                     SVFDHIASHQVDELATYFIESTQRSADARSLPLPFRLSIVGTDGLEHDVDIIPAEHPE
                     VGDGARWVVSVMPVALQASVSRSLEAEMAGAPRQRVKQLLTEELVVDNQHYSSRWFLV
                     DLTGPTGPTVTTARDRDLPMAAAITEQVMHHGWRPWGRAPAGELTPLHAGDLPARLQE
                     IAAEHRWRRISVTPVHVDGTLAAAYLLFGRVPDDYDVTEVNSNVLSRVRRLVDATALL
                     IARWHDRDRLVAAATSDPLTGLANRDAFADALAECAEEATLLYIDVDRFKAVNDQHGH
                     DVGDRVLVEVARRIVAACRPNDVVARFGGDEFVVLLNGVGLERGRQIGERIVEMVAAP
                     NEELADLDLDPVTVSVGLAPVVRRDDAVQAADTAMLRAKRDGRDRLVTADQP"
     gene            complement(6817..7803)
                     /gene="trpS"
                     /locus_tag="VLR27_04370"
     CDS             complement(6817..7803)
                     /gene="trpS"
                     /locus_tag="VLR27_04370"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HSP02710.1"
                     /translation="MVRSFSGIQPSGDLHLGNLLGAITRWVQDQHTQDAVFCVVDLHA
                     LTLPQDPAVLRERTVSLTALLVACGLDPQACTLFLQSHVPEHTRLAWLMECTASFGEL
                     SRMTQFKDKSTRAEFISAGLFTYPALQAADILLYDTDVVPVGDDQRQHIELARDLAQR
                     FNSRAGQDVLVVPKHVIPPVGARIMDLQEPGNKMSKSLESPQGTISVLDDPKAIEKKI
                     KRAVTDADNEVRFDPETKPGVSNLLSILGAATGQDPSTLAADYDMYGPLKSDTAEAVV
                     ELLAPIQARYAELVEDTGTITSILHTGAAKARSVASATLTRVHDAVGLLPPG"
     gene            complement(7857..8960)
                     /locus_tag="VLR27_04375"
     CDS             complement(7857..8960)
                     /locus_tag="VLR27_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSP02711.1"
                     /translation="MGASRILVVDDDPAVCELIRLTLTLEGVEVAIVHDGRSALGHLR
                     SHPVDVVLLDRLMPGMDGLEVLAAMRADPEIAAVSVVLVTGVDGTARAVEGLDTGADD
                     YIVKPFDPDELAARVRSQLRARRTAATTRAGDTAETRAAAARMHRIIQDRAFRAVFQP
                     IVDLSAGEVVGHEALTRFDAGLDPEEVFSTASRLGVGADLERATLSVAIAGARRQTPG
                     TWLALNVTPTLLVGSDALDPILRHAGRDDLVLEISEMEPVHDYVALLEAIDALGASVQ
                     ISVDDAGAGFASLAHILALRARYVKLDKSWIQGIEVEPPKQALVAGLQGFAAETGASL
                     IAEGIETPEQLEAVRSLGIAYGQGYLLGRPGDL"
     gene            9062..>9783
                     /locus_tag="VLR27_04380"
     CDS             9062..>9783
                     /locus_tag="VLR27_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HSP02712.1"
                     /translation="MAVDVETPVFQGPFDLLLHLILQDQVDLYEVRLSDIVDAYLTQL
                     ERLEQMDLELATEFLLIAATLIELKSKRLLPDDGDVDLDDELALWEERDLLLSRLVEC
                     KTFKDAAVAMATIANEAGRSYPRTAGVDQQFLGLLPDMLATTTPDQLRDAFLRAVEPK
                     PVPRVHIDHIHQVRVTVTDAVAELLHELPRIGRVTFAELTADLVERLEVVVQFLAVLE
                     LYKQGWIDLQQVDSLGALTVVWT"
BASE COUNT         1376 a         3437 c         3543 g         1427 t
ORIGIN      
        1 cgggcggcgg cccagtcggc gtggagctcg gcggtctcga accggtcgaa gatctccgcc
       61 atcgccgccc cctgcaacgc cccatggttg ctctcgagcc ggaacgaccc atcgatggcc
      121 gactcctgca ccgacgcgtt ccgtcgctca tggcaccgct ccgcggcctg gtcggcccca
      181 tcggcgtcgg cgagggcttc ccattggcgc agggccgcca cgaagtcgtc gtagccgagc
      241 acgctggcgt ggtgagcgaa gatcggatcc gcgtcgggca cccactcagc caccctcggg
      301 ttcgcgatcg tccgggcgat cgcccgcatg tgctcggtcg ggatctcacc ccgggcgtag
      361 gcggcttcga ccaggggcag gtggggcagg gcccggctgg tctggacccg cccgtgcgcg
      421 tcggatccgg acagccggcc gatcgccttc aacgcgccct tggcggtctt gtgcccgtcg
      481 acgctgaacg ccgcacccac gtcgaggtgg gcgacggtca ggtcggtgcc gtgatccaac
      541 cggcgccgca gcttctccaa ccggcccacc agcgccagct cctcggccgg cgacaggcca
      601 tccagatcga ccgcctggac catccccacc gccacctcga gggccttcaa agcgacatcg
      661 ggatcgacgc cgggttccaa cagatcggtc acggcaacgc ctcccccgag acagcaccgg
      721 tatgtggtcg gtggcagcgc atggcgcggc tccttccttc gagtgtcgac ggtcggcgcg
      781 aagccgacga gtggcacccc gaccccagcc ggagcgccta cccaccattc aactcagcgg
      841 gcctgacagg aactcacggt tcgaccgagc ggagggggcc gttgtctgcc tactgctcga
      901 gcagctctcg ctggcgatgt tcgtggtcga actcccacga cgccaggccg cacaggtgcg
      961 cggcgccacg gtcgtccacc gaggtccaac ggaacccgcg acgccggccg cgatggcgcg
     1021 tggcccgcca ggcacctccg tgctcgtcga gcgccagcgg cggccagccg cgccttcgtg
     1081 ccggcgtgaa gaggtagagc cacagcttcg ggaccgggcc gtcggccggc acggtgccga
     1141 ggtggtcgaa ctggtcgagg gcgacgttga acatggccgg ctgcgcccgg tgccgccgag
     1201 ccaacttcgc caacggctcg tacgccgaca tggggacgtc gaccgatcga gggatgccat
     1261 gggcgtagcc gacgtactcg aagtggtcgt cccagtcgtg accgagggag acctgcacca
     1321 tgcccggcgg gcaggtcggg tcgtgcgggt ggtggcactg gcagaagaag tcgacgtcca
     1381 cgccgtcggg ccgctctcga ccgtcgtcgt ccagcccgcc gtagcactgg agcggctcga
     1441 gcagtgctgg ctcggggtcg ctgtccgagg tcaggcgttc cgtcgccacc aggaatcgca
     1501 gcacagtcca gagatcctcg atccaggtgt ccggcagcag cgcgcggagc ggcgacacct
     1561 cgttccggac cgcaccccac atgatccagt tcacctggcg ccggtcgagg tggatgccat
     1621 cttcggtgcg ctgtcgttcg agtgcgtgga tgcacactgc ggcgacgtcg aggtcgaccc
     1681 gccgcccttc ggccagcgcc cactgggcga gctcggccag caggccgcgg tgctccaccc
     1741 gccggccggc cgcagcctcg gcgaacgggg tggtcgggtg tgcggggacg ggaggcggga
     1801 cctccggcgg cacgtccggg tggagggcgt gcccgcagtg tgagcagtgc tccggcgtgg
     1861 tggtgtactc accacagagg tcgcagaagg acggccatcg gacggcgtct tcggacatga
     1921 cttcactcct gatggatgtg cagtgcaggg gaagccgtgg tggccgggtc ccggccgggg
     1981 tgcatctccc atcctgccga cggggccgga caggaaccgg ccgcgccgac cggtacgagg
     2041 acagcggtgc tggcggacgc gccgtcagcg tgccggagcg acgcgccggg agcagtgcac
     2101 gacgagtcga tccatctgct cggtctggac ctcgacggag tccatgaacg accgcgtgat
     2161 cagcagcccg cgaccgcctc gtcgcaacgg gtcaccgatg tcggtcgcgg tgtgcggtcg
     2221 tcgatccgga tcctcgacga ggttggagac gtcgaggacc atcatgccgt cgtcgatctg
     2281 ggcgcggacc tcgatgtcgt ccgactccgc tggggatccg gcgacggcgt tggcggtgag
     2341 ctcgctgaag acgacctcga ggtcgtcggc gacgtcgcta tcgacatccg cgtcccgcat
     2401 ccagccggcg aagtgcttac ggctcgatgg cagcgcactg accagcgcct tgaaccgttc
     2461 caggaagatg tcggcgggaa cgccgttccg gtgcgtgttc ctgggcatgc ggaccttcgc
     2521 tgggttgtgg cggctccggt acgggagcac gacggttcct accctggtcg gcagggtcct
     2581 acctcggggg cgcgaaggat ccgggggagc gcgggcaccg ggaacccgct tgcccggcga
     2641 tccgggggct gcgaacgtcc gggcgtgacc gaagaccact tcgccgggga ggtcgccgcc
     2701 cgctacgacg ccaccctcgg gcccaagggc accccggaag agctggaccc gacggtcgac
     2761 ctcctggccg agctggtcgg tgacggtgcc gccctcgagc tggcgatcgg caccggtcgg
     2821 gtggcgctgc cgttggcggc gcgaggcgtg gcggtcgcag ggatcgagct gtcacccgac
     2881 atggtcgacc agctgcgggc caagcccggc ggcgccgacc tcgacgtggt catcggcgac
     2941 atgacgaccg cgcgggtcca cggggccttc cggctcgtct acctcgtgta caacaccatc
     3001 gggaacgtcg agacccaggc tcgtcagatc gcgtgcttcg ccaacgcggc tgcgcacctc
     3061 gaaccgggcg gctgcttcgt ggtcgaggtg ggtgttcccg acctccgccg cctggtgccc
     3121 ggccacgacg ccgtcgtctt ctcccacgcg cccggctacg tcggctacga ccggtacgtc
     3181 gatctcgtgg cccagcgagc ggtgtcacac cacttcgtcg ccgatgactc gggtgcgcgc
     3241 gaggtccgga ccccgttccg ctacgtgtgg ccctccgagc tcgacctcat ggcccggctc
     3301 gccggactcc gcctccgcca ccgctgggcc ggctgggacc ggggaccctt caccgacgag
     3361 agcacatccc acgtgtcggt gtgggagaag cccgccgatc ccgggtcggt cggttgaggt
     3421 cggaacccga gccggcgcgt gttgtctatc ctgcggccat gtccgacccc gtcgagcccg
     3481 acaacgacga acgccgcgtg gcgctcgagc aggagcgcga cctcttgcgg tccaaggtgc
     3541 acgagctcga ctccgagggc gacgacctct cgtacgacga caacttcgcc gactcgggcc
     3601 aggtcgccgc cgagcggggc gagaacaagg tcctctacga ccagctccgc cgggacctcg
     3661 acgacatcga gaaggcgctg gagcgcatgg actcgggcac gtacggcacc tgcgaggtct
     3721 gcggggacac catcggtccc gaccgcctcg aggtcatgcc ggccacccgc ttctgcatcc
     3781 agcacgcctg acgccgccgt ggccgggccg atccacctgg tccgccgctt cctcggctcg
     3841 ctgtcgcccg tgccgctgaa ggcggaggac gaggcgtgga cgaacgcaca gctcgggccg
     3901 ggggagcgcc agctctggca gcgcttgtcc cgagccgacc agaagcacgc ggtgggcgtg
     3961 gcgcgggtcg tcgccgaccg ggtcggcgcc gacgagcgag ccgtgatcgc tgccgccctg
     4021 ctccacgaca tcggcaaggt cgaggccgac ctcggcacct tcggacgggt cgcagccacc
     4081 ctcgtccgtc ggcgtcgagg cgcgcgctgg aaggacgcgc cgggcatccg tggccggatc
     4141 gggcgctacc tgcaccacga cgcgatcggc gcgggtctgc tcgccgacgc cggggccgat
     4201 ccgctgacgg tggcctgggc cagggagcat caccggcccg aggacgcctg gaccgtgccg
     4261 gtcgcggtgg gccgggtgct gcgtgacgcc gatgacgact gagcccgagc ggctccgcag
     4321 ctacacggtc gtcggcgccg gggccgtcgg gctcctgtac ggcacccgcc tcgccgtggc
     4381 cggtcacccc gtccggtggc tggtccgctc gggtgccgac acgatggccg cagaaggcat
     4441 cgaggtgacc agcgaagggc gcaccctccg catcgacccc gccgaggtgg tcgtggccac
     4501 tgagccgggt gcgctgccgg cgtccgacgt cgtggtcgtc gcgaccaaga cgaccgcgaa
     4561 cgacaggttg gccgagctgc tcgatggcgc aggggtgccc ggtgcgaccg tcgcggtgtt
     4621 ccagaacggc ctcggggccg aagaggtcgt gcgagaccgc gtcccggagg ccggcgcggt
     4681 gctcggcgcg ctctgcttcg tgtgcgcgca ccgccggggt ccgggtcgcg ccgagcacct
     4741 ggactacggc gccgtcaccc tcgccccgct cgagcccgag cacctggccg ccgccgatgc
     4801 cgtggccgcc gatctgcagc gggcgggcgt ggagacggcg gtgctcgccg acctcggcgt
     4861 ggctcggtgg cgcaagctgg tctggaacat cccgttcaac gggctgtgca cgctgctcga
     4921 cgccagcacc gatgcgctgc tggctgatac cgcctcccga gccctcgttt ccgccctcat
     4981 ggacgaggtc atcgccggtg cccgtgccgt cggccacgac atccccgagg cgttccggga
     5041 cgagatgctg gccgctaccg acgcgatgcg cccgtacgac ccgtcgatga agctcgacat
     5101 cgccgccggg cgcccgttgg agatcgacgc gatctacgac gtcgccaccc ggaccgcccg
     5161 agacgccggc gctccgatgc cccgcaccga ggcgctcgcc gcgatgctcc ggttcagagc
     5221 cggggccgtc tcgtccgggt ccacctgaag ccgtaacgtg ggccggtgga cgcatcctgg
     5281 gcgatctcgg ccctgctcac cctcggcttc ttcctgggag cggtgatgag caccggcgcg
     5341 ctggcggcag ggtggttcgt cctcgccgtc ggcatggctg tgatcaccgt ggtgcgcttc
     5401 cgcgccgcca agcggccccc cggctagggc tggtcggcag tcaccaggcg atcccgtccg
     5461 tcgcgcttgg cgcggagcat ggcggtgtcg gcggcttgga cggcatcgtc gcgccggacc
     5521 acgggggcca ggccgacgct cacggtgacg gggtcgaggt cgaggtcggc cagctcctcg
     5581 ttgggggcgg cgaccatctc gacgatgcgc tcgccgatct gacggccacg ctcgaggccg
     5641 acaccgttca gcagcacgac gaactcgtca ccgccgaagc gggccaccac gtcgttcggc
     5701 cggcatgcgg cgacgatccg gcgggcgacc tcgacgagga cgcggtcgcc gacgtcgtgg
     5761 ccgtgctggt cgttgaccgc cttgaaccgg tcgacgtcga tgtagaggag ggtcgcctcc
     5821 tccgcgcact cggcgagtgc atcggcgaag gcatcgcgat tggcgaggcc ggtgaggggg
     5881 tcgctcgtcg ccgcggccac caggcggtcg cggtcgtgcc agcgggcgat cagcagcgcc
     5941 gtggcgtcga ccagccgtcg cacccgggac aacacgttgc tgttgacctc ggtgacgtcg
     6001 tagtcgtccg ggacccgccc gaacaggagg taggcggcgg cgagcgtgcc gtcgacgtgc
     6061 accggggtga ccgagatgcg acgccagcgg tgctcggcgg cgatctcctg cagcctcgca
     6121 ggcaggtcgc cggcgtggag cggtgtcagc tcaccggccg gtgcccggcc ccacgggcgc
     6181 caaccgtggt gcatgacctg ctcggtgatc gccgccgcca tgggcaggtc ccggtcccgg
     6241 gccgtggtga ccgtcggtcc ggtcggtccg gtgaggtcca cgaggaacca ccgcgagctg
     6301 tagtgctggt tgtccaccac cagctcctcg gtgaggagct gcttgacccg ctggcggggg
     6361 gcaccggcca tctccgcctc caacgagcgg gacaccgacg cctgcagcgc gaccggcatg
     6421 accgacacca cccatcgggc accgtccccg acctcggggt gctcggcggg gatgatgtcg
     6481 acgtcgtgct ccagcccgtc cgtgccgacg atggacaggc ggaacggcag cgggagggac
     6541 cgggcgtcgg ccgagcgctg cgtgctctcg atgaagtacg tggcgagctc gtcgacctgg
     6601 tggctggcga tgtggtcgaa cacgctggcg ccgacccact gggagacgtc gtggccgaag
     6661 aaaccgccgt acccgccgcg cacgtcggcg acggtgccgt cgccgtcgac gaccaggacg
     6721 aggggccgca cggctcggag gatctggtcg gcgtccacgg tctggcgaca gtacccctgg
     6781 gtcaggaccg ctggaccggc ggagcgagag gcgagctcag ccgggtggga ggagcccgac
     6841 ggcgtcgtgc acccgggtga gggtggccga tgccaccgag cgggccttgg ccgcgccggt
     6901 gtgcaggatg gaggtgatgg tgccggtgtc ctcgaccagc tccgcgtagc gggcctggat
     6961 gggggcgagc agctcgacca cggcctcggc ggtgtccgac ttgagcgggc cgtacatgtc
     7021 gtagtcggcg gccagcgtcg aggggtcctg gccggtcgcc gcgccgagga tcgagagcag
     7081 gttcgacacg ccgggcttgg tctcggggtc gaagcggacc tcgttgtcgg cgtcggtcac
     7141 cgcccgcttg atcttcttct cgatcgcctt ggggtcgtcc aggacggaga tggtgccctg
     7201 cggcgactcg agcgacttgg acatcttgtt gcccggctcc tgcaggtcca tgatccgggc
     7261 gcccaccggc gggatcacgt gcttgggcac cacgaggacg tcctgtccgg cgcgcgagtt
     7321 gaaccgctgc gccaggtctc gggccagctc gatgtgctgg cgctggtcgt cgccgaccgg
     7381 cacgacgtcg gtgtcgtaca ggaggatgtc ggcggcctgg agcgccgggt aggtgaacag
     7441 cccggcggag atgaactccg cgcgcgtcga cttgtccttg aactgggtca tccgggacag
     7501 ctcgccgaag ctggcggtgc actccatcag ccacgcgagg cgggtgtgct cggggacgtg
     7561 gctctgcagg aacagggtgc acgcctgggg gtcgaggccg caggcgacga gcagcgccgt
     7621 gagcgacacc gtgcgctccc gaaggaccgc cgggtcctgg gggagggtga gggcgtggag
     7681 gtccaccacg cagaacacgg cgtcctgggt gtgctggtcc tgcacccagc gggtgatcgc
     7741 accgagcagg ttgcccaggt ggagatcgcc ggagggttgg atgccggaga aggagcggac
     7801 catggggcgg catcgtaggg gtggggtccc ggcccctccg agacggttgc cggccgtcag
     7861 aggtcgcccg gccgtccgag gaggtagccc tggccgtagg cgatgcccag cgaccgcacg
     7921 gcctcgagct gctccggggt ctcgatgccc tcggcgatga gcgacgcacc ggtctcggcc
     7981 gcgaagccct ggagacctgc gaccagggcc tgcttcggcg gctccacctc gatgccttgg
     8041 atccaggact tgtcgagctt cacgtacctg gcccgcaggg cgaggatgtg ggcgaggctc
     8101 gcgaacccgg ccccggcgtc gtccaccgag atctgcaccg atgcgccgag cgcgtcgatg
     8161 gcctcgagca gggccacgta gtcgtgcacc ggttccatct cgctgatctc gagcacgagg
     8221 tcgtcacgac cggcgtggcg caggatcggg tccagggcgt cgctgccgac cagcagcgtg
     8281 ggggtcacgt tgagggcgag ccaggtcccg ggcgtctgac gccgagcgcc ggcgatggcc
     8341 accgagagcg tggcgcgctc gaggtcggca ccgacgccga gccggctggc cgtactgaag
     8401 acctcctccg gatcgaggcc ggcgtcgaag cgcgtgagtg cctcgtggcc cacgacctcg
     8461 cccgccgaca ggtccacgat cggctggaac accgctcgga acgcacggtc ctggatgatg
     8521 cggtgcatcc gcgctgccgc cgcccgcgtc tcggcggtgt cgcccgctcg ggtcgtggct
     8581 gcggtgcggc gagcgcgcag ctgggagcgg acgcgtgccg cgagctcgtc gggatcgaag
     8641 ggcttcacga tgtagtcgtc ggcccccgtg tccaggccct ccaccgcccg cgccgtgccg
     8701 tcgacgccgg tgacgaggac caccgacacg gcggcgatct ccgggtcggc gcgcatggcc
     8761 gccagcacct cgaggccgtc catcccgggc atcaggcgat cgaggagcac gacgtcgacc
     8821 gggtgcgacc gcaggtgccc cagcgccgac cgaccgtcgt gcacgatcgc gacctcgacg
     8881 ccctcgaggg tcagggtcaa ccggatcagc tcgcagaccg ctggatcgtc gtccaccacg
     8941 aggatgcgcg aagcccccat atcgggagca tacgctcaaa gtgaccaggg ggtcacggag
     9001 cgtgtgtcgg ccatcgcgac catccaccag cggcgtcggg gtcacgccta cccttggttc
     9061 cgtggccgtc gatgtcgaga ctccggtgtt ccagggtccc ttcgacctgc tcctgcacct
     9121 gatcctgcag gaccaggtgg acctctacga ggtgcggctc tccgacatcg tcgacgccta
     9181 cctgacccag ctcgagcgcc tcgagcagat ggacctggag ctggccaccg agttcctgct
     9241 catcgccgcc acgctcatcg agctcaagtc caagcggctg ctgcccgacg acggcgacgt
     9301 cgacctcgac gacgagctgg ccctgtggga ggagcgcgac ctgctgctct cccgtctggt
     9361 ggagtgcaag acgttcaagg acgcggcggt ggccatggcc accatcgcga acgaggccgg
     9421 acgctcctac ccccgcaccg ccggcgtgga ccagcagttc ctcgggctgc tgcccgacat
     9481 gctcgccacc accacgcccg accagctccg cgacgccttc ctgcgggcgg tcgagcccaa
     9541 gccggtgccc cgggtccaca tcgaccacat ccaccaggtc cgcgtcacgg tcaccgatgc
     9601 cgtcgccgag ctgttgcacg agctgccccg catcggccgg gtcaccttcg ccgagctgac
     9661 cgccgacctc gtcgagcgcc tcgaggtggt cgtgcagttc ctggccgtcc tcgagctcta
     9721 caagcagggg tggatcgacc tccagcaggt cgacagcctc ggcgcgctca ccgtggtctg
     9781 gac
//
LOCUS       DATISU010000104        17785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_51737, whole genome shotgun sequence.
ACCESSION   DATISU010000104 DATISU010000000
VERSION     DATISU010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 17785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17785
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51737"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VLR27_04385"
     CDS             complement(<1..423)
                     /locus_tag="VLR27_04385"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSP02713.1"
                     /translation="MAPEAKAALFDQFAVVGKAFANGRRAELVDVLAQGERAVEALAG
                     QIGQSVANTSQHLQVLARAGLVTSRRDGNRVLYRWADEQVEALWETLRVVAAANVSGV
                     RQAAEDYLGDRDGLEQVTADELARRLGEGVLVWDVRPAS"
     gene            complement(488..676)
                     /locus_tag="VLR27_04390"
     CDS             complement(488..676)
                     /locus_tag="VLR27_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02714.1"
                     /translation="MAVATQTKGRNLAPYLRLRRDDLEVLVARDLVPLAPTLRVSTRR
                     WVTKGFHVDFPGAPACEI"
     gene            861..1019
                     /locus_tag="VLR27_04395"
     CDS             861..1019
                     /locus_tag="VLR27_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02715.1"
                     /translation="MVVLVILIILALIFGVGAVLEGLAWAFLIGLVLLGVAAWWGWSK
                     VRGAAGRR"
     gene            1050..1895
                     /locus_tag="VLR27_04400"
     CDS             1050..1895
                     /locus_tag="VLR27_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HSP02716.1"
                     /translation="MPELDELGARAAELGVRFAVVAGVVVVAVALSRLTAPVVRWLVS
                     RRGRPSRTRVFTALYRVVVVVFGSLAALTLAFPSVRVADVLGIFGILSVAAGFAFKDT
                     FENLLAGVLLLLRDPFKSGDQVVLSELDGTIEGVTVRETLVRGHDGRLFHIPNAEVMR
                     GVIAVETDAPLRRQAIGLRLPADTDLSRAREVLGAALERTTGVEALPPPQVVLTDIVD
                     GDVEIECRFWTGSRRSRATGTRDAALESVLGALREARIGLPVDSVRLDLDGMDGGRSE
                     ARRPR"
     gene            complement(1899..3281)
                     /locus_tag="VLR27_04405"
     CDS             complement(1899..3281)
                     /locus_tag="VLR27_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP02717.1"
                     /translation="MTTSRVLSPLGVRLAAAFVAVAIAAVAVFAGLTLASANDEVSSL
                     VQETHRRDARAAADAAAQAYEEAGGWEGADLSSAAAVAARGQAELIVIDAGGEVLAAP
                     ASDAAEMMARMHGVEILDVDREEPLVQPVAVDGSQVGTVALRFPISHLPGPEREIRQA
                     LVRNAWLGAALAAASAVAVAVVVARVVSRPINALTTAARQVEAGRRDVRVDLDDAPGE
                     IGSLAAAFDRMAASVDEEDRLRRQLVADVAHEVRTPLTILQGTTEALVDGVLPPDPAT
                     LASLHDEVLRLSALVGDLEILAAADAAGLRLEVEGIDLADEVGAVVAGAEASALAADL
                     TLDASLERAPVVGDPRRIRQLATALVANALTYTPSGGSIHIATRSSPDRAVLEVSDTG
                     PGIDAQDLPRIFDRFYRGRRTAGRSGSGIGLAVVRELATAHGGTVTATNGASGGAVFT
                     VDLPADRSPD"
     gene            complement(3271..3966)
                     /locus_tag="VLR27_04410"
     CDS             complement(3271..3966)
                     /locus_tag="VLR27_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP02718.1"
                     /translation="MSATVLVVEDELKIRELLRSYLEREGWSVLTTGSGAEALTITAG
                     AGVDLIILDLGLPDVPGETVAAEVRRHAATPILMLTARAGEEDRIRGLELGADDYVTK
                     PFSPREVVLRAQAILRRSSATPEGGAPRSFGGGTLVIDEARHEVTVRGAPVELTPTEW
                     GILAALAAVPGRVYSRLELLNRVRGYELSSERVVDSHVKNLRRKVEDDPQHPQVVETV
                     LGAGYRLGLAHDH"
     gene            4107..4388
                     /locus_tag="VLR27_04415"
     CDS             4107..4388
                     /locus_tag="VLR27_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02719.1"
                     /translation="MSKILTLTATGLAGLVLGAGIGVAAAGQDATPVAPERSAVTSMD
                     EMHAAMVDQMPAELAEQCDEMHAAMSGHMVGQMGDMPGSHRDHHPDTEG"
     gene            4393..4614
                     /locus_tag="VLR27_04420"
     CDS             4393..4614
                     /locus_tag="VLR27_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02720.1"
                     /translation="MHRSHLRLCGVGLVLALGYVVLTGGSAGGFGLLIAALVCPLAMI
                     VAMTVLMGRDRHQPTAEHADERVEPVQHP"
     gene            4611..5060
                     /locus_tag="VLR27_04425"
     CDS             4611..5060
                     /locus_tag="VLR27_04425"
                     /inference="COORDINATES: protein motif:HMM:NF025170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HSP02721.1"
                     /translation="MTADEAEPVAQHRLVLYLLAPVVVALAVIAGTVAGFASEDAAHG
                     AAPLELAAVPSEVAGHYHAARDLAEVYQEVPCYCGCEEFLAHRDLYDCFVRADGQGWE
                     AHAAGCGVCIAESALVRDLTETGTPVDDIPAAVIDRFGTTPTTAPPT"
     gene            5069..5629
                     /locus_tag="VLR27_04430"
     CDS             5069..5629
                     /locus_tag="VLR27_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02722.1"
                     /translation="MTPHPPKQHTAPHRSHRGVALAGIGALGLFAVIGIGVSVGGEGA
                     APAPSIIETGEVSAMGTPIVETPGSGKGTVAAGPVEVTGASWELGTVPLDVTVRPTWT
                     LRNTGTEPVLVGEPHPEVREGCCPGTFSIDTTTIAPGESATLGFELSMHTGMDGWHDI
                     AVHVPLRAGSTDHVLEVDVNGDFTNT"
     gene            5665..6099
                     /locus_tag="VLR27_04435"
     CDS             5665..6099
                     /locus_tag="VLR27_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HSP02723.1"
                     /translation="MHPRTTALALLTAGSLLLAACGDDDDQMAVDMPTMDEDGHGGDG
                     HDEGNSPVDDGAREIEVTATDFAFDPDEITAEAGEDLAIVLTSEDILHDFTIDELEVH
                     VVADRGETAEGGITADEPGTYDFYCSVPGHREAGMEGTLTVE"
     gene            complement(6115..8232)
                     /locus_tag="VLR27_04440"
     CDS             complement(6115..8232)
                     /locus_tag="VLR27_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HSP02724.1"
                     /translation="MDEDETTDEGVHTTHVTMTAEAIDDAEAHPADHSGHAGHGTDEG
                     HGGGHGGHHGHHVEMFRTRFWWSLLLTVPLVVTSHMVMDWFGYELDFPGIEWVGPILG
                     TIVFLWGGQPFLAGGIEEAKARTPGMMLLIAMAITVAYGASMATSLDLFDLDFWWELG
                     ALISIMLLGHWQEMKALGQAQDALGALAALLPDEAERLTDAGAVETVPASDLRPGDTV
                     LVRSGGRVPADGEIVDGAAELDESMITGESRPVAKGIGDAVVAGTVSTDSSIRVRITA
                     VGDDTALAGIGRLVAEAQESRSRSQVLADKAAALLFYVATGAAVITAVAWSVFGTADE
                     AVVRVVTVLVISCPHALGLAIPLTTSLSSALGARSGILIKDRLALEASRTIDAVLFDK
                     TGTLTRGEHAVVGIAGADGVTEADVLRVAGGVEADSEHPLARAIVTAARDRGEVATAS
                     DFRSITGRGVEATVDGQAYALGGPALLRERSLTVPAELADQVEEWEGRGAAVLHLVRD
                     DRIVGSLALEDQIRPEAREAVEELRARGLQVVMITGDARQVADAVGQDLGLDEVFAEV
                     LPEDKSAKVAELQERGLSVAMVGDGVNDAPALARADVGIAIGAGTDVAIESAGLILAS
                     SDPRGVVAIIRLASGTYRKSLQNLVWAAGYNVVAIPLAAGALHWAGITLPPAVGAVLM
                     SISTIVVALNAQLLRRLDLRVDR"
     gene            8378..9496
                     /locus_tag="VLR27_04445"
     CDS             8378..9496
                     /locus_tag="VLR27_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HSP02725.1"
                     /translation="MTARPTLRWLSALLALALVFTACSSDDDDASSDDSDTTDPGETE
                     TESEITLAQDSSALEAVQEADVLNCGTRDALPGFAVLTEDGEHVGFDADFCRVIAAAI
                     LGDAEKVEFVDLETEARFTALQSGEIDVLVRNTTWTAGRDGSEGATFLQPNFYDGQGM
                     MVRADSEFQSVEDMDGTSVCVASGTTTEGNVSTEFARLGLTVNEVLSFDDVALIQEAF
                     IQGRCDGWSSDVSQLTALRSEFPEGPDALRILPDVFSKEPLAPAVLDGNVDLAEAVNW
                     AVFATIQAEEFGIDSTNIEEFMDSEDPNIRKFLGTDEEGGTFDPGLGLPADFAVQVIT
                     QVGNYAEIFEEHLAPLGLERGENSLWSDGGLLYAPPYR"
     gene            9548..10717
                     /locus_tag="VLR27_04450"
     CDS             9548..10717
                     /locus_tag="VLR27_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSP02726.1"
                     /translation="MTTTATAARPPLWRDVRILRIIGQAAVVAVVAALGLWLWNNVQT
                     NASTTGIPLTLDYLDQPSDITIPGNDLRPTQSVRDAIVAGFWNTIRVSLAGVVLATLI
                     GILIGIARLSSNWLLSRLAKVYVEIFRNVPLLAIIVFSYIAVFLRLPRLEEATTAGSF
                     LIISIRGVAVPWYRIEGSRAAVWAGVAVAVLAAWAVARWRQRVHEATGKPAYRWTAAF
                     LAFAVVLAIGQVLLGSPVSLSGPAVDGRQIAGGITMLPEYAALLAALVVYTASHIAEI
                     VRGSIQAVPKGQVEAASALALSPSQRMRLVVLPQAMRIAVPAIGNQYLNLTKNSSLAV
                     AISYFELTKVTSVSIGNRAPAVPSFAVLLAIYLVLSLAISAVVNIANRRLALVER"
     gene            10714..11919
                     /locus_tag="VLR27_04455"
     CDS             10714..11919
                     /locus_tag="VLR27_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HSP02727.1"
                     /translation="MTAAARWLRVNLFADRRDSAITLVTAAIVAVLGFRLLRFLFVTG
                     RWEIVRVNLALYLYGRYPRDQLWRIVVAVVLIGFLGGVLAGIVARRRDTAARRAGEPV
                     DDRGIGARIGDLTVRVWPLLALVVVLLALSTTIGPSLVALAVLAAIVLGRVLGAALPR
                     GVHVVFFVLPVLTPLALLPFLTGPLGWDSWGGIMLTLLIASVSMIACFPLGLLLALGR
                     RSAKLPAVRTISVGFIEFFRGVPLVVLVLMSDQALGFFFPQSMLPGKVVRAIVVFTIF
                     TAAYVAEIVRGGLQSVPRGQAEAAQALGMAPWRVTGWIVLPQALRNVIPALVGQFISL
                     FKDTTLVFVIGLLDPLTVADNVTKQSDFELSNFPEALIFAGLLFWVGSYTMSRESQRL
                     ERRLGVGTR"
     gene            11916..12695
                     /locus_tag="VLR27_04460"
     CDS             11916..12695
                     /locus_tag="VLR27_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HSP02728.1"
                     /translation="MTDTGVYSDATQAGGSGEVMIELEGVDKFFGDFQALKDINLRVG
                     KQEVVVVCGPSGSGKSTMIRCINRLEEHDRGRIVIDGTELSDDLRNIQAIRRETGMVF
                     QSFNLFPHLTVLDNITLAPRQVRKLPRAEAEARAHELLERVKIPEQAEKFPGQLSGGQ
                     QQRVAIARALAMQPTVMLFDEPTSALDPEMIGEVLDTMRQLAQDGMTMIVVTHEMGFA
                     REVADRVLFMADGEVVEVGTAEHFFTNPQEERTKLFLSQIL"
     gene            12911..14446
                     /locus_tag="VLR27_04465"
     CDS             12911..14446
                     /locus_tag="VLR27_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HSP02729.1"
                     /translation="MPSGFTAPNDLITPSALDDGPARGRLARFGGGWGVDIKANLLAG
                     LVVAVALIPEAIAFSIIAGVDPKVGLYASFSIAVIISFTGGRPAMISAATGAMALVMI
                     PLVRDHGVEYLFAATIVTGVVQLGLGALGVGRLMRFIPRTVMVGFVNALAILIFLAQL
                     DHFVGEAWGVWAMIAAGLGIIYGVPRVTTAVPAPLVAIVALTSAAFVFDLGLPTVGDM
                     GELPTALPFFGLPDVPLTFETLSIVAPFAITLALIGLLESLLTAQLIDDITDTRSDKD
                     RESRGQGIANVATGFLGGMAGCAMIGQSMINVKSGGRSRLSTFSAGIFLLVMILVFGD
                     LVAMIPMAALVAVMIVVAVATFDWTSLKPSTIRSTPKTETAVMGVTVLTVVLTHNLAY
                     GVIAGVVLATIFFARRVAHLVDVTSVLDPEGGERVYALTGELFFASTNELVHSFAYDD
                     DVERVVIDLTDAHVWDSSAVATLDAVVHKFEARGIEVELIGLNDHSHELHNGLTGRLV
                     NGH"
     gene            14522..15283
                     /gene="modA"
                     /locus_tag="VLR27_04470"
     CDS             14522..15283
                     /gene="modA"
                     /locus_tag="VLR27_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HSP02730.1"
                     /translation="MRRLAILTAGVTAALALAACGDDATSDPAAGGSITVFAAASLSD
                     AFTEVADRFEVANGGVSVELSFAGSSSLREQILGGAPADVFASADEADMDRLVEAGEV
                     QPPEVFATNRLQIVVPAGNPAGVEGLDDFADSDLLIGLCAEEVPCGHLGREVLGEAGV
                     TPTPDTDEPDVRALLTKVEAGELDAGLVYVSDVVAAGDAVEGIDLPAEVDIVASYPIA
                     ATTRSEQPDAARAFVEFVLGAEGQAILERSGFERS"
     gene            15280..16071
                     /locus_tag="VLR27_04475"
     CDS             15280..16071
                     /locus_tag="VLR27_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP02731.1"
                     /translation="MSRRRTRSQAPWVLLPLAGLGVAFIVLPLVALLQRTPWSDLGSL
                     LGDPVVTDALGLSLISALVATALSLLVGVPLAWVLARTDFRGRSVVRALVTLPMVLPP
                     VVGGAALLFAFGRRGLLGEPIYDGTGFLLPFSLWGVIAANTFVAMPFLVITVEAGLRS
                     ADQRYEDAAATLGASRWTIFRRITVPNAMPAVLAGAVLCWARALGEFGATVTFAGNLQ
                     GRTQTMPLAVFVALESDRDAAIALSLVLIAVSLAVLIPLRDRWLR"
     gene            16068..17132
                     /locus_tag="VLR27_04480"
     CDS             16068..17132
                     /locus_tag="VLR27_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP02732.1"
                     /translation="MSTLDADIRLTLGELDLDVALRADTGEAVAVLGPNGAGKTTIVS
                     ALAGLVPIDDGHITLDGTVLDHPDRGTFVPSERRPIGVVFQDYLLFPRMDARDNIAFG
                     LRARGADKATAHEQAERWLDRFGLTEHARSRPSALSGGQAQRVALARALATNPKLLLL
                     DEPLAALDASTKVHVRAELRRHLSTFEGARILVTHDPIDALILADRLVIVEDGRITQA
                     GTPADITSRPATRYVADLVGINLLHGRLDGSTVHLDEGGELTVAAADDVPRGDVALAI
                     RPQTIALYAERPDGSPRNVWSATIADLEADRDRIRVRLAGPIDVTAEVTATAAHTLDL
                     RPGQQVWASIKAVDVTVYER"
     gene            complement(17133..>17785)
                     /locus_tag="VLR27_04485"
     CDS             complement(17133..>17785)
                     /locus_tag="VLR27_04485"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02733.1"
                     /translation="VSGVEDARFVRFEDDDRVVYYATYTAYDGRDIGQQLLETVDFCT
                     FEASPLQGSAASNKGLALFPRRIDGHFVALSRHDGTSNAVAVTDDIRHWPTATALGGR
                     TRLWSSIQVGNCGPPIETEAGWLVLTHGVGPMRTYSIGACLLDLDDPTRMIGELAEPL
                     LTPGPDEQDGYVPNVIYSCGAVRHGPNLVIPYGVGDSSIGFATADIDDVIGAMSGC"
BASE COUNT         2447 a         6702 c         5981 g         2655 t
ORIGIN      
        1 ggacgcaggg cggacgtccc agaccaggac gccctcgccc agccgccggg cgagctcatc
       61 ggcggtcacc tgctccaacc cgtcccggtc accgaggtag tcctcggcgg cttggcgcac
      121 gccggagacg ttggcggcgg ccacgacgcg cagcgtctcc cacagcgcct ccacctgctc
      181 gtcggcccac cggtacagca cgcggttgcc gtcccggcgc gacgtcacca gcccggcgcg
      241 ggccaggacc tgcaggtgct gggaggtgtt cgccaccgac tggccgatct ggccggccag
      301 cgcttcgacg gcccgttcgc cctgggcgag cacgtccacg agctcggccc gacggccgtt
      361 ggcgaacgcc ttgcccacca ccgcgaactg gtcgaacagc gctgccttcg cctctggtgc
      421 cacgaggaca ccatacaccg atttctcaac tactcaagcg agttcttgat cagatgcgcc
      481 agcgcgctca gatctcacac gccggcgcgc cggggaagtc cacgtggaac cccttggtca
      541 cccagcggcg cgtggagacg cgcagcgtcg gcgccagcgg gacgaggtcc cgggcgacga
      601 gcacctcgag gtcgtcacgc cggagccgga ggtagggcgc caggttgcgc cccttcgtct
      661 gggtggcgac cgccacctct gcgaccttgc ctcaaccggt ggggcggatg aggtcgatca
      721 ccagcgtctt gccccgcttg gacaggaggg cggcggcgtc gtcggtcagc tcgatctgca
      781 tgcttccccc aaccctgcag ccggcggtcg tgttcccttc gaccgactgc accgcagtgg
      841 tgccgggtac gggtcagaac atggtcgtcc tcgtcatcct catcatcctc gccctgatct
      901 tcggggtcgg cgccgtcctc gagggtctcg cctgggcgtt cctcatcggc ctcgtgctgc
      961 tgggcgtcgc cgcctggtgg ggttggtcca aggtccgtgg cgcagccggt cgccgatgac
     1021 cgctgaccgc gctcggtcgg cctgcgcaga tgcctgagct cgacgagctg ggggcacggg
     1081 ccgccgagct cggtgtccgg ttcgccgtcg tggccggcgt cgtcgtcgtc gcggtcgcgc
     1141 tcagcaggct gacggcacct gtcgtccgct ggctcgtgtc tcggcgcggc cgtccgagcc
     1201 gcacgcgcgt gttcaccgcg ctgtaccggg tcgtggtggt cgtcttcggc agcctcgccg
     1261 cactgaccct cgccttcccg tccgtgcgcg tcgccgacgt gctcgggatc ttcggcatcc
     1321 tgtcggtcgc cgccggcttc gcgttcaagg acaccttcga gaacctcctc gccggtgtgc
     1381 tgctcctgct ccgagacccc ttcaagtcgg gtgaccaggt ggtgctgtcc gagctcgacg
     1441 ggacgatcga gggcgtcacc gtgcgggaga ccctcgtccg gggccacgac ggccgtctgt
     1501 tccacatccc caacgccgag gtgatgcggg gcgtcatcgc cgtcgagacc gacgcacccc
     1561 tgcgccgcca ggccatcggg ctgcgactgc cggccgacac cgacctgagc cgtgcccggg
     1621 aggtcctggg tgcggcgctg gagcggacga cgggagtgga ggcgcttcca cctccgcagg
     1681 tggtgctgac cgacatcgtc gacggggatg tcgagatcga gtgccgcttc tggaccggct
     1741 cccgtcgcag ccgggccacc ggcacgaggg acgccgccct cgagtcggtg ctcggcgccc
     1801 tccgggaggc acgcatcggc ctccccgtcg actccgttcg gctcgacctg gacgggatgg
     1861 atggcgggag atccgaggct cggcgtccgc gctgagcgtc agtcgggcga ccggtcggcc
     1921 ggcaggtcga ccgtgaagac cgcgccgccc gacgccccgt tcgtggcggt gacggtgccg
     1981 ccgtgggcgg tggccagctc gcggaccacg gcgaggccga tcccgctgcc cgagcggccg
     2041 gcggtccggc gcccccggta gaagcggtcg aagatccggg gcaggtcctg ggcgtcgatg
     2101 cccggcccgg tgtcggacac ctcgagcacc gctcggtccg gcgacgaccg ggtcgcgatg
     2161 tggatcgagc cacccgacgg cgtgtaggtc agcgcgttgg cgacgagggc ggtggcgagc
     2221 tgtcggatcc ggcgtgggtc gcccacgacc ggtgcccgct cgagggaggc gtccagcgtg
     2281 aggtcggcgg cgagcgccga tgcttccgcc cccgccacga cggctccgac ctcgtcggcc
     2341 aggtcgatgc cttcgacctc gagtcgcaga ccggccgcat cggccgccgc aaggatctcc
     2401 aggtcgccga cgagcgcgga caggcggagc acctcgtcgt gcaacgaggc gagcgtcgcc
     2461 ggatcgggtg ggagcacccc gtcgacgagc gcctcggtcg tgccctgcag gatcgtcaac
     2521 ggcgtgcgca cctcgtgcgc gacgtcggcg acgagctggc ggcggagtcg atcctcctcg
     2581 tcgaccgacg cggccatccg gtcgaacgcg gccgccaggc tgccgatctc gcccggggcg
     2641 tcgtcgaggt ccacccggac gtcgcgccgt ccggcctcga cctggcgagc cgcggtggtg
     2701 agggcgttga tcggccggct gaccactcgg gcgacgacca ccgccacggc caccgctgac
     2761 gcagccgcga gggcggcacc gagccaggcg ttgcggacca gcgcctgtcg gatctcgcgc
     2821 tcgggaccgg ggaggtggct gatcgggaac cgcagcgcga cggtgccgac ctggctgccg
     2881 tccaccgcga cgggctggac gaggggttcc tcccggtcga cgtcgaggat ctcgacgccg
     2941 tgcatgcggg ccatcatctc ggctgcgtcg ctcgccggcg cggcgaggac ctcgccgccc
     3001 gcgtcgatga cgatgagctc ggcctgacct cgtgctgcga ccgctgccgc gctcgacagg
     3061 tctgccccct cccagccgcc cgcctcctcg taggcctgcg cggctgcgtc ggccgcggcc
     3121 cgtgcgtctc ggcggtgggt ctcctggacg aggctggaga cctcgtcgtt ggcgctggcc
     3181 aaggtcagcc cggcgaagac ggcgacggcg gcgatcgcga cggcaacgaa cgccgccgcg
     3241 agtcggacgc cgagcggaga caggacccgg ctagtggtca tgggccagcc cgaggcggta
     3301 gccggcgccg agcacggtct cgacgacctg ggggtgttgg ggatcgtcct cgaccttgcg
     3361 gcgcaggttc ttcacgtggc tgtcgacgac ccgctcggag gacagctcgt acccccgcac
     3421 ccggttgagc agctcgagcc gcgagtagac gcggccgggc accgcagcca gggccgccag
     3481 gatcccccac tccgtcggcg tcagctcgac gggtgcgccc cggaccgtga cctcgtggcg
     3541 ggcctcgtcg atgacgagcg tccccccacc gaagctgcgc ggcgccccgc cctcgggggt
     3601 ggcgctcgat cgacgcagga tcgcctgggc ccgcagcacg acctcgcgcg ggctgaacgg
     3661 cttggtcacg tagtcgtcgg caccgagctc caggccgcgg atccggtcct cctcaccggc
     3721 ccgcgccgtg agcatcagga tcggcgtcgc tgcatggcgc cgcacctcgg cggcaacggt
     3781 ctcgcccggg acatcaggga gcccgaggtc gaggatgatc aggtcgacgc cggcgccggc
     3841 ggtgatggtc agggcttcgg cgccgctgcc cgtcgtcagc accgaccagc cttcacgctc
     3901 gaggtacgaa cggagcagct cgcggatctt cagctcgtcc tccacgacga ggaccgtggc
     3961 gctcatggcg ccttgcctac ctgattccga ccacgggccg tgctgcctcg ggagcgattc
     4021 ctcccaggtt ccatgccatc tccacaccgt ctccacaccg agcgccgaca gtggcagcaa
     4081 cgacaggagc tgccaggagg caccagatgt ccaagatcct caccctcact gcgaccggcc
     4141 tcgccgggct ggtgctcggc gccggcatcg gcgtcgccgc cgccggccag gacgccaccc
     4201 cggtggcccc cgagcggagc gccgtcacgt ccatggacga gatgcacgcc gccatggtcg
     4261 accagatgcc ggccgagctg gccgagcagt gcgacgagat gcacgccgcc atgagcgggc
     4321 acatggtcgg gcagatgggc gacatgcccg gcagccaccg cgaccaccac cccgacacgg
     4381 agggctgacg acatgcaccg atcccatctc cgcctgtgcg gcgtcgggct ggtcctggcg
     4441 ctgggctacg tcgtcctcac cggcggctcc gctggcggct tcgggttgct catcgccgcg
     4501 ctcgtgtgcc cactggcgat gatcgtcgcg atgaccgtgc tcatgggccg ggacaggcac
     4561 cagccgaccg ccgaacacgc cgacgaacgc gtcgagccgg tccagcaccc atgacggccg
     4621 atgaggccga gccggttgcc cagcatcggc tcgtcctcta cctcctcgca ccggtcgtcg
     4681 tcgcgctcgc ggtgatcgcc ggcacggtcg ccgggttcgc cagcgaagac gccgcacacg
     4741 gggccgcgcc cctcgagctg gcggcggtgc ccagcgaggt cgctgggcac taccacgcgg
     4801 cccgcgacct ggccgaggtg taccaggagg tgccctgcta ctgcgggtgc gaggagttcc
     4861 tggcccaccg cgacctctac gactgcttcg tccgcgccga cggccagggc tgggaggccc
     4921 atgctgccgg ctgcggcgtg tgcatcgccg agtccgcgct cgtgcgggac ctgaccgaga
     4981 cgggcacgcc cgtcgacgac atccccgccg cggtcatcga ccgcttcggc accaccccga
     5041 ccaccgcccc acccacgtga aggccgccat gaccccgcac cccccgaagc agcacaccgc
     5101 tccccaccgc agccaccgag gagtcgcgct cgccggcatc ggcgccctcg gcctgttcgc
     5161 cgtcatcggc atcggcgtct ccgtcggcgg cgagggcgcc gcaccggccc cctcgatcat
     5221 cgagaccggc gaggtctccg ccatgggcac acccatcgtc gagacgcccg gatccggcaa
     5281 aggcaccgtc gccgccgggc cggtcgaggt gaccggcgcc agctgggagc tcgggaccgt
     5341 gcccctcgac gtgaccgtcc gcccgacctg gacgctgcgc aacaccggca ccgaacccgt
     5401 cctcgtcggg gaaccgcacc ccgaggtccg cgaggggtgc tgccccggca ccttcagcat
     5461 cgacaccacc accatcgccc cgggcgagtc cgccaccctc ggcttcgagc tgtcgatgca
     5521 cactgggatg gacggctggc acgacatcgc cgtccacgtc cccctgcgag ccgggtccac
     5581 cgaccacgtg ctcgaggtcg acgtcaacgg cgacttcacc aacacctgac acacgccccc
     5641 cgaccctcga aggaaccgat ccccgtgcac ccacgaacca ccgccctggc gctcctcacg
     5701 gccggcagcc tcctgctcgc cgcgtgcggt gacgacgacg accagatggc cgtcgacatg
     5761 ccgaccatgg acgaagacgg ccacggcggc gacggccacg acgagggcaa cagcccggtg
     5821 gacgatggcg cccgggagat cgaggtgacc gccacggact tcgccttcga tcccgacgag
     5881 atcaccgccg aggccggtga ggacctcgcc atcgtgctca cgagcgagga catcctccac
     5941 gacttcacca tcgacgagct cgaggtccac gtcgtcgccg accgtggtga gaccgccgaa
     6001 ggcggcatca ccgccgacga gcccgggacc tacgacttct actgctcggt gccgggccat
     6061 cgtgaggccg gcatggaggg aacgctcacc gtcgagtgag acccgatcgc agcgtcagcg
     6121 gtcgacgcgc aggtccagcc ggcgcagcag ctgggcgttg agggcgacca cgatcgtcga
     6181 gatgctcatc agcacggcac cgacggcggg cggcagggtg atgccggccc agtgcagggc
     6241 gccggcggcg agggggatgg ccacgacgtt gtagccggcc gcccacacga ggttctggag
     6301 cgacttgcgg taggtgccgc tcgccaaccg gatgatggcg acgacaccgc gcgggtcgga
     6361 gctggccagg atcaggccgg cggactcgat ggcgacgtcc gtgccggcgc cgatggcgat
     6421 gccgacgtcg gcgcgagcca gggcgggggc gtcgttgact ccgtcgccga ccatggccac
     6481 cgacaggccg cgctcctgga gctcggccac cttggccgac ttgtcctcgg ggaggacctc
     6541 ggcgaagacc tcgtcgaggc ccaggtcctg accgacggcg tccgcgacct ggcgggcatc
     6601 gccggtgatc atcacgacct ggagcccacg ggcccgcagc tcctccacgg cctcgcgggc
     6661 ctccgggcgg atctggtcct cgagggcgag ggatccgacg attcgatcgt ctcgcacgag
     6721 gtggaggacg gccgcgccgc gaccctccca ctcctcgacc tggtcggcga gctcggcggg
     6781 cacggtcagg gaccgctccc gcagcagcgc cgggccgccg agggcgtagg cctgcccgtc
     6841 cacggtcgcc tcgacccctc gaccggtgat cgagcggaag tcgctcgccg tcgcgacctc
     6901 gccccggtca cgggcggcgg tgacgatggc ccgagccagc gggtgctcgc tgtcggcctc
     6961 gacgccgccg gcgacccgca ggacatccgc ctcggtgacc ccgtcggcgc cggcgatgcc
     7021 gaccacggcg tgctccccac gggtgagcgt gcccgtcttg tcgaacagca cggcatcgat
     7081 cgtgcggctc gcctccagcg ccaggcggtc cttgatgagg atgccgctgc gggcaccgag
     7141 ggcgctcgac agcgacgtcg tcagcgggat cgccagcccg agggcgtgcg ggcaggagat
     7201 cacgaggacg gtcacgaccc gcacgacggc ctcgtcagcg gtgccgaaga ccgaccaggc
     7261 cacggcggtg atcaccgcgg ctccggtggc gacgtagaag agcagcgcgg cggccttgtc
     7321 ggcgaggacc tggctgcgcg accgggactc ctgggcctcg gccacgagcc ggccgatccc
     7381 ggccagggcg gtgtcgtcac ccaccgccgt gatccgcacc cggatggacg agtccgtcga
     7441 gacggtgccg gcgacgaccg cgtcgccgat gcccttggcg accggacggg actcgccggt
     7501 gatcatcgat tcgtcgagct cggcggcgcc gtcgacgatc tcaccgtccg ccggaacccg
     7561 gccgccggag cggaccagga ccgtgtcgcc gggacgcagg tccgacgcgg gcacggtctc
     7621 gacggcgccg gcgtcggtga ggcgctcggc ctcgtccggg aggagcgcgg cgagcgcgcc
     7681 gagggcgtcc tgggcctggc cgagcgcctt catctcctgc cagtgcccga gcagcatgat
     7741 cgagatcagc gcaccgagct cccaccagaa gtccaggtcg aacaggtcca gcgacgtcgc
     7801 catcgacgcc ccgtaggcga cggtgatcgc catggcgatc agcagcatca tccccggcgt
     7861 gcgggccttc gcctcctcga tcccgccggc gaggaacggc tgaccgcccc acaggaacac
     7921 gatcgttccg aggatcggcc cgacccactc gatgccgggg aagtcgagct cgtagccgaa
     7981 ccagtccatg accatgtggc tggtcacgac cagcggcacg gtcagcagca ggctccacca
     8041 gaaccgcgtc cggaacatct cgacgtggtg gccgtggtgg cccccgtgcc cgccaccgtg
     8101 cccctcgtcg gtcccgtggc ccgcgtgacc gctgtggtcg gcggggtggg cctcggcgtc
     8161 gtcgatggcc tcggcggtca tggtcacgtg cgtcgtgtgc acgccttcgt cggtggtctc
     8221 gtcctcgtcc atggacctac catacccccc caccctatcc tgtcaacccc caccgggccc
     8281 gcttctgccg ttcccccgac acgagatccg gacccggtgt tatgttccgg ccaccggcgg
     8341 gcctgtgaac ggactgccac tgaatggggg aatccacatg acggcacgac cgacgcttcg
     8401 ttggctctcg gcgctgctcg cactcgcact cgtcttcact gcgtgcagca gcgacgacga
     8461 cgacgcgagc agcgacgaca gcgacaccac cgacccgggg gagaccgaga ccgagtccga
     8521 gatcacgctg gcccaggaca gcagcgccct ggaggcggtg caggaggccg acgtcttgaa
     8581 ctgcggcacg cgcgatgcac tgcccggctt cgccgtgctc accgaggacg gcgagcacgt
     8641 cggcttcgac gccgacttct gccgggtcat cgcggcggcg atcctcggcg atgcggagaa
     8701 ggtcgagttc gtggacctcg agaccgaggc acgattcacc gccctgcagt cgggcgagat
     8761 cgacgtcctg gtgcgcaaca cgacgtggac cgccggccgc gatggctccg agggcgccac
     8821 gttcctgcag ccgaacttct acgacggcca ggggatgatg gtccgggccg acagcgagtt
     8881 ccagtccgtc gaggacatgg acggcacgtc ggtgtgcgtg gcgtcgggca ccaccaccga
     8941 gggcaacgtc tccaccgagt tcgcccggct cggcctcacc gtcaacgagg tcctgtcctt
     9001 cgacgacgtg gcgctgatcc aggaggcctt catccagggc cgctgcgacg gttggtcctc
     9061 cgacgtgtcc cagctcaccg ccctgcgctc ggagttcccc gagggtccgg acgccctgcg
     9121 catccttccc gacgtgttct cgaaggagcc gctcgccccg gccgtgctcg acggcaacgt
     9181 cgatctcgcc gaggccgtga actgggccgt cttcgccacc atccaggccg aggagttcgg
     9241 gatcgactcg acgaacatcg aggagttcat ggacagcgag gaccccaaca tccgcaagtt
     9301 cctcggcacc gacgaggaag gcggcacgtt cgatccgggt ctcgggctgc cggcggactt
     9361 cgcggtccag gtgatcaccc aggtcggcaa ctacgccgag atcttcgagg agcacctcgc
     9421 acccctggga ctcgagcgcg gcgagaactc gctctggtcg gatggcggcc tgctctacgc
     9481 accgccgtac cgctgagcca cgagctgagc gaccgaccgg atcgggctca ccctcggtga
     9541 gccagcgatg accacgacgg cgacggcagc acgaccaccg ctctggcgcg acgtccggat
     9601 cctccggatc atcggccagg cggcggtcgt cgccgtcgtc gccgcgctcg gcctctggct
     9661 gtggaacaac gtccagacga acgcgtccac gaccgggatc ccgctcaccc tcgactacct
     9721 ggaccagccg agcgacatca ccatcccggg caacgacctg cggcccaccc agagcgtgcg
     9781 cgacgccatc gtcgccgggt tctggaacac gatccgcgtg tccctcgccg gcgtggtgct
     9841 cgccaccctc atcggcatcc tcatcggcat cgcgcggctg tcgagcaact ggctgctctc
     9901 gcggctggcg aaggtctacg tggagatctt ccgcaacgtg ccgctgctcg ccatcatcgt
     9961 cttctcctac atcgcggtgt tcctccggtt gccccgcctg gaggaggcga ccacggccgg
    10021 gagcttcctc atcatctcga tccgcggcgt ggccgtcccg tggtaccgga tcgagggcag
    10081 ccgagccgcg gtctgggccg gggtggcggt cgccgtgctc gcggcgtggg ccgtcgcccg
    10141 gtggcgtcag cgggtgcacg aggccaccgg gaagccggcc taccggtgga ctgcggcgtt
    10201 cctcgcgttc gcggtcgtcc tcgcgatcgg gcaggtcctc ctcggctcac cggtgtcgct
    10261 gtccggcccg gccgtcgacg gtcgacagat cgccggtggc atcacgatgc tgccggagta
    10321 cgccgcgctc ctcgccgccc tggtcgtcta caccgccagc cacatcgccg agatcgtgcg
    10381 aggcagcatc caggcggtcc ccaagggcca ggtggaggcg gcgtccgcgc tggccctctc
    10441 gccctcccag cgcatgcgcc tggtcgtgct gccccaggcc atgcggatcg ccgtgccggc
    10501 catcggcaac cagtacctga acctgacgaa gaactcctcg ttggccgtcg ccatcagcta
    10561 cttcgagctc acgaaggtga cctcggtgag catcggcaac cgagcacccg ccgtgccgtc
    10621 cttcgccgtg ctcctcgcca tctacctcgt gctctcgctg gcgatctcgg ccgtggtcaa
    10681 catcgccaac cgccgcctcg ccctggtgga acgatgaccg cggccgcccg ctggctccgg
    10741 gtcaacctct tcgccgaccg ccgggactcg gccatcaccc tcgtcaccgc cgcgatcgtg
    10801 gcggtcctcg gcttccgcct gctgcggttc ctcttcgtga ccggacggtg ggagatcgtc
    10861 cgggtcaacc tcgccctgta cctctacggc cggtacccgc gggaccagct gtggcgcatc
    10921 gtcgtggccg tcgtgctcat cggcttcctc ggtggcgtgc tggccggcat cgtcgcccgg
    10981 cgtcgtgaca ccgcggcgcg ccgggcgggc gagccggtgg acgaccgcgg catcggcgcc
    11041 cgcatcggcg acctcacggt gcgggtctgg ccgctactgg ccctcgtcgt cgtcctgctg
    11101 gcgttgtcga ccacgatcgg cccgagcctc gtcgccctgg ccgtgctcgc cgcgatcgtg
    11161 ctcggccgcg tgctcggtgc cgcgctcccc cgcggcgtcc acgtcgtgtt cttcgtgctg
    11221 ccggtgctga cgccgttggc cctgctgccg ttcctcaccg gaccgctcgg ctgggacagc
    11281 tggggcggca tcatgctcac cctcctcatc gcatcggtga gcatgatcgc ctgcttcccg
    11341 ctcgggctgc tgctggccct cgggcggcgc tcggcgaagc tgccggcggt ccgcaccatc
    11401 tccgtcgggt tcatcgagtt cttccgcggc gtccccctcg tcgtcctcgt gctgatgagc
    11461 gaccaggcgc tcggattctt cttcccccag tcgatgctgc ccggcaaggt cgtccgggcc
    11521 atcgtcgtct tcaccatctt caccgccgcg tacgtggccg agatcgtccg gggcgggctc
    11581 cagtccgtcc cgcgcggcca ggccgaggcg gcgcaggcgc tcgggatggc gccgtggcgg
    11641 gtgacgggtt ggatcgtcct gccccaggcc ctgcgcaacg tcatccccgc cctcgtcggc
    11701 cagttcatca gcctgttcaa ggacacgacc ctggtcttcg tcatcggcct gctcgacccg
    11761 ctcaccgtgg cggacaacgt caccaagcag tccgacttcg agctgtcgaa cttccccgag
    11821 gcactgatct tcgccggcct gctgttctgg gtcggctcgt acacgatgtc gcgcgagagc
    11881 caacggctcg agcgccgatt gggagtggga acacgatgac cgacaccggt gtgtacagcg
    11941 acgcgacgca ggccggaggc tccggcgagg tcatgatcga gctcgagggg gtcgacaagt
    12001 tcttcggcga cttccaggcg ctcaaggaca tcaacctgcg ggtcggcaag caggaggtcg
    12061 tggtcgtctg cgggccgtcc gggtccggga agtcgacgat gatccgctgc atcaaccggc
    12121 tggaggagca cgaccgcggg cgcatcgtga tcgacggcac cgagctgtcc gacgaccttc
    12181 gcaacatcca ggccatccga cgggagaccg gcatggtctt ccagtcgttc aacctgttcc
    12241 cgcacctgac ggtcctcgac aacatcacgc tcgcgccccg ccaggtccgc aagctccccc
    12301 gcgccgaggc cgaggcccgg gcccacgagc tgctcgagcg ggtcaagatc cccgagcagg
    12361 ccgagaagtt ccccggccag ctgtccggtg gtcagcagca gcgcgtcgcc atcgcccgcg
    12421 ccctcgcgat gcagcccacg gtcatgctgt tcgacgagcc gacgtcggcc ctcgacccgg
    12481 agatgatcgg cgaggtcctc gacaccatgc gccagctcgc ccaggacggc atgacgatga
    12541 tcgtcgtgac ccacgagatg gggttcgccc gcgaggtggc cgaccgggtc ctgttcatgg
    12601 ccgacggcga ggtcgtcgag gtcggcacgg cggagcactt cttcaccaac ccgcaggagg
    12661 agcgcacgaa gctcttcctc agccagatcc tgtagacggg atcggcaccg gacccgcatc
    12721 ccttcgcgca cgttccggta ggttgcgacc cggcaccggc tgaccgccgt gcccagcgag
    12781 tcctctggtg aagccgtgcc gccctgctgc ggcggccgac tccggcctcc gggctcgccc
    12841 cccactgacc tcccctggtt cgtcgccgcc ccccgttcgt gcgcgagctc gagaacccga
    12901 aagcgcacgc atgccctccg gcttcaccgc ccccaacgac ctcatcacgc ccagcgccct
    12961 ggacgacggc ccggcccgcg gccggctcgc tcgcttcggt ggcggctggg gcgtcgacat
    13021 caaggcgaac ctgctcgccg gcctcgtcgt cgccgtggcc ctcatccccg aggccatcgc
    13081 gttctcgatc atcgccggcg tcgatcccaa ggtcggcctc tacgccagct tcagcatcgc
    13141 ggtgatcatc tcgttcaccg gcgggcggcc cgccatgatc tccgccgcca ccggggccat
    13201 ggccctcgtg atgatcccgc tggtgcggga ccacggggtc gagtacctgt tcgccgccac
    13261 gatcgtcacc ggcgtggtcc agctcggact gggcgccctc ggggtcggtc gcctgatgcg
    13321 gttcatcccc cgcaccgtga tggtcggctt cgtgaacgcc ctcgccatcc tcatcttcct
    13381 ggcccagctc gaccacttcg tcggcgaggc ctggggcgtc tgggcgatga tcgccgccgg
    13441 tctcggcatc atctacggcg tcccccgcgt caccaccgcc gtgccagcac cgctggtggc
    13501 catcgtcgcc ctgaccagcg ccgccttcgt cttcgacctc ggcctcccga ccgtcggcga
    13561 catgggcgag ctgccgacgg ccctgccctt cttcggcctc cccgacgtgc ccctcacctt
    13621 cgagacgctg tcgatcgtcg ctcccttcgc catcaccctg gccctcatcg gcctgctcga
    13681 gtcgctgctc accgcgcagc tcatcgacga catcaccgac acccgctccg acaaggaccg
    13741 cgagtcgcgc ggccagggca tcgccaacgt cgcgaccggc ttcctcggcg gcatggccgg
    13801 ctgcgccatg atcggccagt ccatgatcaa cgtgaagtcc ggtggccgca gccgcctgtc
    13861 caccttctcg gccggcatct tcctgctcgt catgatcctg gtcttcggcg atctggtcgc
    13921 catgatcccc atggccgcac tcgtcgccgt gatgatcgtc gtcgcggtcg ccaccttcga
    13981 ctggacgagc ctcaaacctt cgacgatccg ctcgaccccc aagaccgaga ccgcggtcat
    14041 gggcgtcacc gtcctcaccg tcgtgctcac ccacaacctc gcctacggcg tcatcgccgg
    14101 cgtcgtgctc gccacgatct tcttcgcccg gcgggtcgcc cacctcgtcg acgtcaccag
    14161 cgtcctcgac cccgagggcg gcgagcgggt ctacgccctc accggcgagc tgttcttcgc
    14221 ctccaccaac gagctcgtcc actccttcgc ctacgacgac gacgtcgagc gagtggtcat
    14281 cgacctcacc gacgcgcacg tctgggactc ctcggccgtg gccaccctcg acgccgtggt
    14341 ccacaagttc gaggcccgtg gcatcgaggt cgagctcatc gggctcaacg accacagcca
    14401 cgagctgcac aacggcctca ccgggcggct cgtgaacggc cactgacgcc ggcatcgggc
    14461 agcgaccggg ctgccctgcg ttaggtttca ccgccccagc ggatcgcgga ggatgggcac
    14521 gatgcgacgg ttggccatcc tcacggctgg cgtgacagcg gccctcgccc tggcggcgtg
    14581 cggcgatgac gccacgtcgg acccggcggc gggcggctcg atcacggtgt tcgcagccgc
    14641 atcgctgtcc gatgccttca ccgaggtggc ggaccggttc gaggtcgcca acgggggcgt
    14701 gtcggtcgag ctgagcttcg ccggttcgtc gtcgctgcgc gagcagatcc tgggcggggc
    14761 cccggccgac gtgttcgcct ccgccgacga ggcggacatg gaccggctgg tcgaggccgg
    14821 ggaggtccag ccacctgagg tcttcgccac caaccggttg cagatcgtcg tgccggccgg
    14881 caacccggcc ggtgtggagg gcctcgacga cttcgcggat tccgacctgc tgatcgggtt
    14941 gtgcgccgag gaggtcccct gcgggcacct cgggcgggag gtcctcggcg aggccggcgt
    15001 gacaccgact cctgacaccg acgaacccga tgtccgagcg ctgctcacca aggtcgaggc
    15061 cggcgagctc gacgccgggc tcgtctacgt gagcgacgtg gtcgccgcag gcgacgcggt
    15121 ggagggcatc gatctccccg cggaggtcga catcgtcgcg tcctacccga tcgccgccac
    15181 cacccgatcc gagcaaccgg atgcggcccg ggcgttcgtc gagttcgtgc tcggcgccga
    15241 gggccaggcc atcctcgagc ggtcgggctt cgagcgatcg tgagccgacg tcgcaccagg
    15301 tcccaggcac cgtgggtgct gctgcccctc gccggcctcg gggtggcttt catcgtcctg
    15361 ccgctggtgg cgctcctgca gcgcacgccg tggagcgatc tcggatccct cctcggtgac
    15421 ccggtcgtca ccgatgccct cggcctcagc ctgatcagcg ccctcgtcgc caccgcgctc
    15481 tcgctgctcg tgggtgtccc cctcgcctgg gtgctcgccc gcaccgactt ccgaggccgg
    15541 tcggtcgtgc gcgccctggt cacgctgccc atggtgctgc cgcccgtcgt cgggggcgcc
    15601 gccctgctgt tcgcgttcgg ccggcggggc ttgctcggcg aaccgatcta cgacggcacc
    15661 ggcttcctgc tccccttctc gctctggggg gtgatcgccg ccaacacctt cgtggccatg
    15721 ccgttcctgg tcatcaccgt cgaagcgggg ctgcgctcgg cggatcagcg ctacgaggac
    15781 gccgccgcca cgctcggcgc cagccgctgg accatcttcc ggcgcatcac ggtccccaac
    15841 gccatgcccg ccgtgctcgc cggcgccgtg ctgtgctggg cccgcgccct gggcgagttc
    15901 ggcgcaaccg tcaccttcgc cggcaacctc cagggccgca cccagacgat gccgctcgcc
    15961 gtgttcgtcg ccctggagtc cgaccgcgac gccgccatcg ccctgtccct ggtgctgatc
    16021 gcagtctccc tcgccgtgct catcccgctc cgcgatcgct ggctgcgatg agcacgctcg
    16081 acgccgacat ccggctgacg ctcggcgagc tcgacctcga cgtcgcgctc cgcgccgaca
    16141 ccggcgaggc cgtcgccgtc ctcggcccga acggtgccgg caagaccacc atcgtcagcg
    16201 ccctcgcggg cctggtcccg atcgacgacg gccacatcac cctcgacggc accgtcctcg
    16261 accatcccga ccgtggcacc ttcgtcccga gcgaacgtcg tcccatcggc gtcgtgttcc
    16321 aggactacct cctcttcccc cggatggacg cccgggacaa catcgcgttc gggctccgag
    16381 cgcgcggtgc cgacaaggcc accgcccacg agcaggccga gcggtggctc gatcgcttcg
    16441 ggctcaccga gcacgcccgg tcgcgaccat cggcgctctc gggtgggcag gcccagcggg
    16501 tggcgctggc ccgcgcgctc gcgacgaacc cgaagctcct gctcctcgac gaacccctgg
    16561 ccgcgctcga cgccagcacc aaggtccacg tccgggccga gctgcgccgc cacctgagca
    16621 ccttcgaggg cgcccgaatc ctcgtcacgc acgacccgat cgacgcgctg atcctcgccg
    16681 accggctggt catcgtcgag gacggccgca tcacccaggc cggcacgccc gccgacatca
    16741 ccagccggcc ggcgacccgc tacgtggccg acctcgtggg catcaacctc ctccacggcc
    16801 gcctcgacgg cagcacggtg cacctcgacg agggaggcga gctcaccgtg gcagccgccg
    16861 acgacgtccc ccgcggcgac gtggccctcg ccatccgccc ccagaccatc gccctctacg
    16921 ccgagcgccc cgacggcagc ccccgcaacg tctggtcggc cacgatcgcc gacctcgagg
    16981 ccgaccggga ccgcatccgg gtccgactcg ccgggccgat cgacgtcacc gccgaggtca
    17041 ccgccactgc cgcccacacc ctcgatctgc gccccggcca gcaggtctgg gccagcatca
    17101 aggccgtcga cgtcaccgtc tacgagcgct gatcagcagc cggacatcgc gccgatcaca
    17161 tcgtcgatgt ccgcggtggc gaacccgatg gaggagtcgc cgaccccgta ggggatcacc
    17221 aggttcggac cgtggcgcac cgcgccgcag gagtagatga cgttgggcac gtagccgtcc
    17281 tgctcgtccg gaccgggggt cagcagcggc tcggcgagct cgccgatcat gcgcgtcgga
    17341 tcgtcgaggt ccagcaggca ggcgccgatc gagtacgtgc gcatggggcc gacgccatgg
    17401 gtcagcacca gccagccggc ctccgtctcg atcgggggtc cgcagttgcc cacctggatc
    17461 gagctccaga gcctcgtccg accgcccagc gccgtcgccg tgggccagtg gcggatgtcg
    17521 tcggtgaccg cgaccgcatt gctcgtgccg tcgtggcgcg atagcgccac gaagtggccg
    17581 tcgatgcgcc gagggaacag ggcgaggccc ttgttgctgg cggcggaacc ctgcagcggg
    17641 gacgcctcga aggtgcagaa gtcgacggtc tcgaggagct gctggccgat gtcgcgcccg
    17701 tcgtaggcgg tgtaggtggc gtagtagacg acgcggtcgt cgtcctcgaa gcggacgaag
    17761 cgagcatcct cgacgccgct cacct
//
LOCUS       DATISU010000105         5303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_703706, whole genome shotgun sequence.
ACCESSION   DATISU010000105 DATISU010000000
VERSION     DATISU010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703706"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VLR27_04490"
     CDS             complement(<1..215)
                     /locus_tag="VLR27_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02734.1"
                     /translation="MRRLLHGLLGATLLLGACGGDDDDGRAAAITSIPEETSTSTSST
                     TTEPRTVAPDVIPQDVDLITEEYVEQVL"
     gene            331..1143
                     /locus_tag="VLR27_04495"
     CDS             331..1143
                     /locus_tag="VLR27_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02735.1"
                     /translation="MASKRPNKQQRQRQNRQRREARQARSVHAGEATAIARGEREAAE
                     PTSSNRTAPKTRSEKAAQARAERKSRYSIPGQRAVVMAFLFTIVSAATLLIAPVQVQR
                     EVPIDDPVLEDIDEDDQEVDEDAGTVTYVDDAKLLDEEGPAVAAGVMLTPILITGAAL
                     WFTKKPQRSTAWTMAMIALAAYIFLFAGPYGIITLPSMIALAVGGFQSRRAESKERLA
                     EIRAKKAATGGGEVIDVESSEVTEPDEVDVDEVEPEATEVEPAEPVGDDDRR"
     gene            1186..1659
                     /locus_tag="VLR27_04500"
     CDS             1186..1659
                     /locus_tag="VLR27_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HSP02736.1"
                     /translation="MSEAAEAAHAKFQTLIGEESEPGEWLQVTQEMINQFADVTMDHQ
                     FIHVDPEAAKNTPFGGTIAHGFLTLSLLVPLSSSIKQDPETVKGVIMGVNYGFDKIRF
                     IAPVPSGSRIRAKSVLKSAEIKGSSVNATRTYTIEIEGSDKPAVIADWITRLTYG"
     gene            complement(1675..2274)
                     /locus_tag="VLR27_04505"
     CDS             complement(1675..2274)
                     /locus_tag="VLR27_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HSP02737.1"
                     /translation="MPSRRPRALGLVVAAAVALAGCNDDGRTLEPTSATMPPSEVPAT
                     TPADDVTGLRVTSPDVVEGGALDPAFTCDGAGLAPLLVISGAPPAAAELAVAVVDLDA
                     ADFVHLVVAGLPSTTAQVDPNQPPDGAVVARTDSDVLGWDAPCPPPGDAAHRYEIRLY
                     AAAEPLGLAPGLPGHEAIGLIEAAAIDVARLGFTYAAAG"
     gene            complement(2292..3257)
                     /locus_tag="VLR27_04510"
     CDS             complement(2292..3257)
                     /locus_tag="VLR27_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HSP02738.1"
                     /translation="MALIIVLAVLGFFLFVYVAAGVRIIKPYQRGIIERLGKYKETVD
                     PGLRLIIPLVDTMRVVDMREQVVDVPPQEVITEDNVGVSVDAVIYYEPTDPQRLIYNV
                     ANFILAVTKLAQTNLRNVIGDLPLDESLTSRDKINIALREILDDATDKWGVRVVRVEI
                     QRIDPPPDVMTAMHEQMKAERTRRAVVTEAQGRREASITTAEGATAAAILEAEGSKAK
                     SVLEAEGRAEAVRKYAEAEQYRQSAVAEGEASAIRSVYQAIHDGNPSNDLLAIKYLET
                     LQKLGDGQATKIIVPTELGGLAGALAGVTELLERSGANGRTDAKT"
     gene            complement(3257..3748)
                     /locus_tag="VLR27_04515"
     CDS             complement(3257..3748)
                     /locus_tag="VLR27_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HSP02739.1"
                     /translation="MDDPDFWRWVWLAAAVTFGLGEMASAGTFFLAPFALGATGAAVA
                     SFLGAPVGLGWLVFVLVSVVAFAALRPLAARLDATSSNPLGVGAKRLVGEHGVVLTEV
                     PAGPDELGSVRIGREEWRAEALDGGPLAPGTHVTVLEVRGTRVIVYPTGLPSSTRPPE
                     RPH"
     gene            complement(3801..4532)
                     /locus_tag="VLR27_04520"
     CDS             complement(3801..4532)
                     /locus_tag="VLR27_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02740.1"
                     /translation="MPSRRSTARRRMAVVLAVLGLGGALAGCGDDTDEVAATGDERAE
                     QAETAASEAGLEDEVAEFLGLLARGETATYRVEFPGPVEGTELLIANRPPDRRVEVVA
                     DGTTTELRLVIEGQAFTCTPSAEEDELTCERTDALVEPPGVFRAEALDELSEALAERS
                     DDYSFEIETLTVAGVEARCLVTRLRSGRESAELGSSGTICASPEGAVLLVDQGSDRLE
                     ATDYGTDVADDAFLRPDRPEDDADS"
     gene            4569..4874
                     /locus_tag="VLR27_04525"
     CDS             4569..4874
                     /locus_tag="VLR27_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02741.1"
                     /translation="MAPPATTEPTSRTLGRSVALGIGIGVGAAAICFFLIAIPFYTLA
                     SFEPNGIDRPIVRTGLFRIALPVGLLVGLASGVASTLWLRRGGSWTVSDGSDRYSNR"
     gene            4885..>5303
                     /locus_tag="VLR27_04530"
     CDS             4885..>5303
                     /locus_tag="VLR27_04530"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSP02742.1"
                     /translation="MSPTAERCILTVHAHPDDESSKGAATIARCKAEGIRTVLVCCTD
                     GAAGDILNPAMDSEDVKLNLPAVRRDELAQAARIIGYDEVIDLGYRDSGMPDTDANAH
                     PEAFANADPDEAIGKLVAILRRERPQVVVTYGDDQRG"
BASE COUNT          796 a         1840 c         1932 g          735 t
ORIGIN      
        1 agcacctgct cgacgtactc ctcggtgatc aggtccacgt cctgagggat cacgtcgggg
       61 gcgaccgtgc ggggctcggt cgtggtcgac gaggtggagg tgctcgtctc ctcggggatc
      121 gaggtgatcg cggcggcacg accgtcgtcg tcatcgcctc cgcaggcgcc gagcaggagg
      181 gtggcgccga gcaggccgtg gaggagccgc cgcatggcag gaatgtagtc agcacaacgt
      241 gacctgaaaa gatccttcag tccctttcac cccatgtggg tgggacgtga atggggcggc
      301 cccgaccgtg ccctctaccg tcggtgcacg atggcttcga agcgaccgaa caagcagcag
      361 cgtcagcggc agaaccgcca gcggcgggag gcccgccagg cccggtcggt gcacgccggt
      421 gaagcgaccg ccatcgcccg cggcgagcgc gaggcggccg agccgacctc cagcaaccgg
      481 acggcgccca agacccgcag cgagaaggcc gcccaggccc gcgccgagcg caagagccgc
      541 tacagcatcc ccggccagcg ggccgtggtg atggccttcc tgttcacgat cgtgtcggcg
      601 gcgaccctgc tgatcgcgcc ggtgcaggtg cagcgcgagg tccccatcga cgacccggtg
      661 ctcgaggaca tcgacgagga cgaccaggag gtcgacgagg acgccggcac cgtcacctac
      721 gtcgacgacg ccaagctgct cgacgaggag ggcccggccg tggccgccgg cgtcatgctc
      781 acgccgatcc tcatcaccgg cgcggccctg tggttcacga agaagcccca gcgctcgacc
      841 gcgtggacca tggccatgat cgccctggcc gcgtacatct tcctgttcgc tggcccgtac
      901 gggatcatca ccctgccctc gatgatcgct ctggccgtcg gcggcttcca gtcccgccgg
      961 gccgagtcca aggagcgcct ggcggagatc cgtgcgaaga aggcagcgac cggtggcggc
     1021 gaggtcatcg acgtcgagtc gagcgaggtc accgagcccg acgaggtgga cgtcgacgag
     1081 gtcgagcccg aggccaccga ggtcgagccg gcagagcccg tgggcgacga cgaccgccgc
     1141 tagggtcgcc ccgccatcga gaccaccgca cgaggagacc tgaagatgtc ggaagccgca
     1201 gaggccgccc acgccaagtt ccagaccctg atcggcgaag agagcgagcc gggtgagtgg
     1261 ctgcaggtca cccaggagat gatcaaccag ttcgccgacg tgacgatgga ccaccagttc
     1321 atccacgtcg accccgaggc ggcgaagaac acccccttcg gcggcaccat cgcccacggc
     1381 ttcctgacgc tgtccctgct cgtgccgctg tccagctcga tcaagcagga cccggagacg
     1441 gtcaagggcg tgatcatggg cgtgaactac ggcttcgaca agatccgctt catcgctccc
     1501 gtcccgagcg gctcccgcat ccgggccaag tcggtgctca agtcggccga gatcaagggc
     1561 agctcggtca acgcgacccg cacctacacg atcgagatcg aaggctccga caagcccgcc
     1621 gtcatcgccg actggatcac ccggctcacg tacggctgat ccgcctgctg ccgctcaacc
     1681 agcggcggcg taggtgaacc cgaggcgagc cacgtcgatg gctgccgcct cgatcaggcc
     1741 gatggcctcg tgcccgggga gcccgggcgc gaggccgagc ggttcggctg cggcgtagag
     1801 gcggatctcg tagcggtggg cggcatcgcc cggaggcggg cacggtgcgt cccagccgag
     1861 cacgtcggag tccgtgcgtg ccacgacggc gccatccgga ggctggttcg ggtccacctg
     1921 ggccgtggtg ctcggcaggc cggcgaccac gaggtggacg aagtcggctg catcgaggtc
     1981 gacgaccgcg acggcgagct cggctgccgc cggcggcgcc ccgctgatca cgaggagcgg
     2041 ggcgagtccg gccccgtcgc aggtgaaggc cgggtcgagc gcgccaccct cgaccacgtc
     2101 cgggctggtg acgcgcagcc cggtcacgtc gtccgccggc gtggtggcgg ggacctcgct
     2161 cggcggcatc gtggccgagg tcggctccag ggtgcggccg tcgtcgttgc agccggcgag
     2221 cgcgacggca gccgcgacga cgagaccgag cgcgcgcggg cgacgggacg gcatcgaggt
     2281 gatcgggcgg atcaggtctt ggcgtcggtg cggccgttgg cgccgctgcg ctcgagcagc
     2341 tcggtgaccc cggccagcgc gccggccagg ccacccagct cggtgggcac gatgatcttg
     2401 gtcgcctgac cgtcgccaag cttctgcagg gtctcgaggt acttgatggc gagcaggtcg
     2461 ttggacgggt tgccgtcgtg gatggcctgg tacacggacc ggatggcgga ggcttcaccc
     2521 tcggcgaccg cgctctggcg gtactgctcg gcctcggcgt acttgcgcac ggcctcggcc
     2581 cgaccttcgg cctccagcac cgacttggcc ttggagccct cggcctcgag gatcgccgcg
     2641 gccgtggcgc cttcggcggt ggtgatcgac gcctcgcgcc gaccctgggc ctcggtgacc
     2701 acggcacgcc gggtgcgctc ggccttcatc tgctcgtgca tggcggtcat gacgtccggc
     2761 ggcggatcga tccgctggat ctcgacccgg accacccgca cgccccactt gtcggtggcg
     2821 tcgtcgagga tctcgcgcag ggcgatgttg atcttgtccc gggaggtcag cgactcgtcc
     2881 aggggcaggt cgccgatgac gtttcgcagg ttggtctggg ccagcttggt gacggcgagg
     2941 atgaagttgg cgacgttgta gatgagccgc tgcgggtcgg tcggctcgta gtagatgacc
     3001 gcgtcgacgg agacgccgac gttgtcctcg gtgatgacct cctggggcgg cacgtcgacg
     3061 acctgctcgc gcatgtccac cacgcgcatg gtgtcgacca gcgggatgat gaggcggagg
     3121 ccgggatcga cggtctcctt gtacttgccg agccgctcga tgatgccgcg ctggtagggc
     3181 ttgatgatgc gaacgccggc ggcgacgtag acgaacagga agaagccgag gacggccagg
     3241 acgatgatga gggccactag tgaggccttt ccggtggtcg ggtggagctg gggaggccgg
     3301 tggggtagac gatgacccgc gtgccgcgga cctcgagcac ggtgacgtgg gtgccggggg
     3361 cgagcggtcc gccgtcgagg gcttcggccc gccactcctc gcgcccgatg cgcaccgagc
     3421 cgagctcgtc ggggccggcg ggcacctcgg tgagcacgac gccgtgctcg ccgacgaggc
     3481 gcttggcccc gacgccgagc gggttgctcg acgtggcatc gaggcgagcg gccagcgggc
     3541 gcagcgcggc gaacgccacg accgacacga ggacgaagac cagccagccg aggccgaccg
     3601 gggcgccgag gaacgacgcg accgcggcgc cggtggcgcc gagggcgaag ggcgcgagga
     3661 agaaggtgcc cgctgaggcc atctcaccga gaccgaaggt cacggcagcc gccagccaga
     3721 cccaccgcca gaagtcgggg tcgtccacga gggcgatggt accgatcggg gacggtcgat
     3781 gtgccccggt tcgtgccgga tcaggagtcg gcgtcgtcct cgggccggtc ggggcgcagg
     3841 aaggcgtcgt cggcgacatc ggtcccgtag tcggtggcct ccaggcggtc gctgccctgg
     3901 tcgaccagga gcacggcacc ctccggcgac gcgcagatgg tcccgctcga tccgagctcg
     3961 gccgactccc gcccgctgcg gagccgggtg accaggcacc gggcctcgac accagcgacg
     4021 gtcagcgtct cgatctcgaa ggagtagtcg tcggaccgct cggcgagggc ttcgctcagc
     4081 tcgtcgaggg cctcggcccg gaagacaccg ggcggctcga ccagggcgtc ggtccgctcg
     4141 caggtgagct cgtcctcctc ggccgatggc gtgcaggtga acgcctggcc ctcgatcacc
     4201 aggcgcagct cggtggtcgt gccgtcggcc acgacctcga cacgacggtc gggcgggcgg
     4261 ttcgcgatca ggagctcggt gccctcgacc ggacccggga actcgacccg gtaggtcgcg
     4321 gtctcgccgc gggcgaggag cccgaggaac tccgcgacct cgtcctcgag gccggcctcg
     4381 ctcgccgcgg tctcggcctg ctcggctcgc tcgtcgccgg tggcggcgac ctcgtcggtg
     4441 tcgtcgccgc accccgcgag cgccccgccg aggccgagca ccgccaggac gacggccatc
     4501 cggcgtcggg cggtcgatcg gcggctgggc acggccggat ggtacggacc ggcagtaccg
     4561 ttggcggaat ggcacccccc gccacgaccg aacccacgtc ccggaccctc ggccgctccg
     4621 tcgcgctcgg catcggcatc ggcgtcggcg cggcggcgat ctgcttcttc ttgatcgcca
     4681 tccccttcta cacgctcgcc tccttcgagc cgaacggcat cgaccgtccg atcgtgcgca
     4741 ccggcctgtt ccggatcgcg ctgccggtgg gcctcctcgt cgggttggcc tcgggtgtcg
     4801 catcgacgct gtggctccgc cgcggtggct cctggacggt cagcgacggg agcgaccgct
     4861 acagcaaccg ttagcgttgg cctggtgagc cccaccgctg agcgctgcat cctgaccgtc
     4921 cacgcccacc ccgacgacga gtcctcgaag ggcgctgcga ccatcgctcg atgcaaggcg
     4981 gagggcatcc gcacggtgct ggtgtgctgc accgacggtg cggccgggga catcctcaac
     5041 ccggcgatgg acagcgagga cgtgaagctg aacctgccgg cggtgcggcg cgacgagctg
     5101 gcccaggccg ccaggatcat cggctacgac gaggtcatcg acctcggcta ccgggactcc
     5161 ggcatgcccg acaccgatgc caacgcccac cccgaggcct tcgccaacgc cgaccccgac
     5221 gaggccatcg gcaagctcgt cgccatcctg cgccgcgagc gcccgcaggt ggtggtgacc
     5281 tacggcgacg accagcgcgg cta
//
LOCUS       DATISU010000106         1920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_377860, whole genome shotgun sequence.
ACCESSION   DATISU010000106 DATISU010000000
VERSION     DATISU010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377860"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..729
                     /locus_tag="VLR27_04535"
     CDS             16..729
                     /locus_tag="VLR27_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HSP02743.1"
                     /translation="MAADQIILTAETGRPTGSRSSRRLRAEGKIPAVVYGLDTEPKPI
                     AVEWSELRAALVTDAGMNALIDLDVDGEKKLTMVYDMQRHPVRRDVTHVDFILIDVNK
                     TIDVDVPIVLAESDDDHLKGLVIDQNLFMLTVSAKPGTIPNEFLVDLAPLSVDEPIRA
                     GQIELPDGVELLTDPEDAVVVASVPMSEEELLADVEDLAGDVDEPGEGEEAAEGEEGA
                     EAEGGDDAEASADEGGEEA"
     gene            783..1352
                     /gene="pth"
                     /locus_tag="VLR27_04540"
     CDS             783..1352
                     /gene="pth"
                     /locus_tag="VLR27_04540"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HSP02744.1"
                     /translation="MIGLGNPGLQYARTRHNVGADCVALLADRNGERLAHSRKEHAEV
                     AELRPTPTMRRLVVAFPTTYYNEAGRAARALVLRHGIDDPTKVVVVHDELDLPVGRLK
                     VKAGGGLAGNNGLRSIKANLQTDEFLRIRIGVDKPPNSQQGADHVLRKPGKREREELD
                     VCIAEAADAVEMILVDGIDRAMERYNRKE"
     gene            1432..>1920
                     /locus_tag="VLR27_04545"
     CDS             1432..>1920
                     /locus_tag="VLR27_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HSP02745.1"
                     /translation="MTVAESLAPTDDLRHLPTVAREEPGLLAVLGRKDAVLAVPEPAR
                     ALVVAGLAARSTRRPIVVATAATADAERLVNDLQALLGADAVEHFPAWETLPFERVSP
                     AIETMGRRLTTLHRLGAGRDDPAQLPDVVVTSVRALIQRLAPGVEDIEPVCITKGDEV
                     DIE"
BASE COUNT          311 a          718 c          658 g          233 t
ORIGIN      
        1 cttgtaggta gacccatggc agcagaccag atcatcctca cggccgagac cgggcgcccc
       61 accggctccc gctcctcgcg ccgcctccgc gccgagggca agatcccggc ggtcgtctac
      121 ggcctcgaca ccgagcccaa gcccatcgcc gtggagtgga gcgagctgcg tgctgccctg
      181 gtcaccgacg ccggcatgaa cgccctgatc gacctcgacg tcgacggtga gaagaagctg
      241 acgatggtct acgacatgca gcgccacccg gtgcgccgcg acgtcaccca cgtcgacttc
      301 atcctcatcg acgtcaacaa gaccatcgac gtcgacgtgc ccatcgtgct cgccgagagc
      361 gacgacgacc acctcaaggg cctggtcatc gaccagaacc tgttcatgct cacggtctcg
      421 gccaagccgg gcaccatccc gaacgagttc ctggtcgacc tggccccgct gtcggtggac
      481 gagcccatcc gcgccggcca gatcgagctg cccgacggcg tcgagctgct caccgatccc
      541 gaggacgccg tcgtggtcgc gtcggtgccc atgtccgagg aagagctgct ggccgacgtc
      601 gaggacctgg ccggcgacgt ggacgagccc ggcgagggcg aagaggccgc cgagggcgaa
      661 gagggtgccg aggccgaggg cggcgacgac gccgaggcct ccgccgacga gggcggcgag
      721 gaagcctgag cagcccgttc gccgaggctg ccgaccggcg ggggaccccc gccgacctgt
      781 tggtgatcgg cctcggcaac ccagggctcc agtacgcacg cacccgccac aacgtgggcg
      841 cggactgcgt cgcgctgctg gcggaccgca acggcgagcg cctcgcgcac tcgcgcaagg
      901 agcacgccga ggtcgccgag ctgcgaccga cgcccaccat gcgccgcctc gtcgtcgcgt
      961 tcccgaccac gtactacaac gaggcggggc gggccgcccg ggccttggtc ctgcgccacg
     1021 gcatcgacga cccgaccaag gtcgtggtgg tccacgacga gctcgacctg cccgtcggcc
     1081 ggctcaaggt gaaggccggc ggcggcctcg ccggcaacaa cggcctgcgg tccatcaagg
     1141 caaacctcca gaccgacgag ttcctgcgca tccgcatcgg cgtggacaag ccgccgaaca
     1201 gccagcaggg cgccgaccac gtgctgcgca agccgggcaa gcgcgagcgc gaggagctcg
     1261 acgtctgcat cgccgaggcc gccgacgccg tcgagatgat cctggtcgac ggcatcgacc
     1321 gggccatgga gcgctacaac cgcaaggagt agatcgttcg ttggtccgga cgtgaccaaa
     1381 gacggtcgat cgctgaccag ggaacggtgc agcgtcgata gcctcggttc ggtgaccgtc
     1441 gcagagtccc tcgcccccac ggacgacctg cggcacctcc ccaccgtcgc ccgggaagag
     1501 cccgggctgc tggccgtgct cggccgcaag gacgcggtgc tggcggtgcc ggaaccggcg
     1561 cgcgccctgg tcgtggccgg cctggcggcg cgctccaccc ggcggcccat cgtggtcgcc
     1621 accgcggcca ccgccgacgc cgagcggctg gtcaacgacc tgcaggcgct gctcggcgcc
     1681 gacgccgtcg agcacttccc ggcgtgggag accctgccct tcgagcgggt cagcccggcc
     1741 atcgagacca tggggcgccg gctcacgacc ctgcaccgcc tgggcgccgg ccgggacgac
     1801 cccgcccagc tgcccgacgt cgtggtcacg tccgtgcggg cgctcatcca gcgcctcgcc
     1861 cccggcgtcg aggacatcga gccggtgtgc atcaccaagg gcgacgaggt cgacatcgag
//
LOCUS       DATISU010000107        10318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_470926, whole genome shotgun sequence.
ACCESSION   DATISU010000107 DATISU010000000
VERSION     DATISU010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10318
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470926"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VLR27_04550"
     CDS             complement(<1..887)
                     /locus_tag="VLR27_04550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSP02746.1"
                     /translation="MIDTIHQVVPSLTPGDGVGGHTLAVRDVLRSAGLASEIFSDDVH
                     PALAGQARPLDALPEPGTPGLALLYQCSIGNEVVDRLLARPEPLVVDYHNLTPVEHLL
                     RWAPDMAHLAGWGRSQLGDLARRAVLGIGDSAFNTRELEAAGFARTAVVPILLDVRGL
                     RHDGAGSGRRAGRWLFVGRVVPNKAQHDLVLALAWHRAVHDPDAQLVLVGRDSAPAYT
                     DAVRDLITELDLEGAVHLVGGVDDARLAREYAEADVFVCASEHEGFCIPVLEAMASGL
                     PVVAYASTALPETVGSGGIL"
     gene            complement(884..1663)
                     /locus_tag="VLR27_04555"
     CDS             complement(884..1663)
                     /locus_tag="VLR27_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02747.1"
                     /translation="MAESPDPWRAVVDVAGAADAERAAHPELAARLAAAGSRFGSARH
                     DVRGLLDDVDRRAVIDVDAPVDAARPVVPQLKWSVRKAVAFVARHLAQQTTAVVSGLS
                     AAVRLLDERVGRLEVHGLDAVIAGAPDVRPALVDALGALEAGASGVRRDVGSTVELAT
                     LPAGDAGLVVAYRLLDVGPLPSRLAALDQLVRGVAAGGRLAVVSAAPDAWAELADPVT
                     RDLGEPGPVHAETWAHLLEARGGSDVEVHRGDGAFVVAARW"
     gene            complement(1656..3758)
                     /locus_tag="VLR27_04560"
     CDS             complement(1656..3758)
                     /locus_tag="VLR27_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HSP02748.1"
                     /translation="MRSGAAAAIVSLALASAAWSAPSVEATVADGSSRWDVTVPGGRV
                     HWSSPAIADVDGDAASDVVVGGLDGRVHAYDADGRPLPGWPANASIVPGTRSAVASSP
                     AVGDVDGDGRDEVVVGTGSLEVPDQHGGVVVLEGDGSTRCRFQTGDKFDQWSGGGPDG
                     FSDAVWASPALGDVTGDGRIDIVIGSWDHQIRVLDGACRQVAAFDNTDTVWSAPALFD
                     ADGDGRDEIYIGGDATSNHGSHSGGFFRALRYAGGPQLQQRWVRLSSEAFQTAPAIAD
                     INGDGRLEVVTGAGRYYCQSAQRCHDSNKVWAFDLLTGRDVPGWPRTARYNTFLAAPA
                     VGDVNADRQRDVVIGSFLPGRGAVLALRGDGTPLWEVEPASDELTSPPVIADVLPDPG
                     NEVIVASGGRVHVLDGHTGKTLRSLGAGAYKNAAAVGELGRGRWALVAAGFQPGNGNA
                     ATIQAFDIATPAETPWPMYGATPDRRRVFPLGPPPTPRPIDPVCPEDEVPSSGVRDVP
                     PSSPHHRAVECAVWWEISQPVDGMYRPAQSVTRAQMASFVARLIDAAGAPLPEDPPDA
                     FGDDDGAAYEPHEHNIDRLAAVDVVAGRTATSYAPGAAVTRGQMATFLVRAHRHVTGT
                     TLPLTRSWFHDRDPVHGDNVDRAAGAGIATGYANGTYRPGADVRRDQMASFLMRTAAV
                     LDSSGSWEGRSASMSRHG"
     gene            3815..5995
                     /locus_tag="VLR27_04565"
     CDS             3815..5995
                     /locus_tag="VLR27_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HSP02749.1"
                     /translation="MTVTGRRPNVLVVLFDDVGFSDFGCYGSEIATPTIDRLASAGLR
                     YSGFHTTAMCSTTRAALLTGRNHHNVGVGCLANFDSGLPGYRGKISREAGTVAELLDP
                     HGYRCYLTGKWHVTPLTETGPTGPYDGWPLQRGFHRFYGFLDAETDQYAPELVHDNHH
                     VDPPATFEEGYHLSADLADKTVEFLRDHRYGVPDEPWLAWLAFGACHAPHQAPRELID
                     HYEGVFAKGWDVTRQGRLERQIALGLVPAGTELPPRNDRVRAWDDHSADEQRVFTRLQ
                     AAYAAMLDHADQQLARVVAFLEETDQLDDTLVLVLSDNGASQEGGPSGFVNAMGPFNA
                     QPEPLEAKLATLDAVGGPGTHANFPWGWAMAANTPLKRYKQNTHGGGIRDPLVVSWPV
                     GIGSRGEVRHQFCHAVDLVPTILDVLGTEPPAAIAGVTQLPMDGASMRSTFDDADAPP
                     PKDVQYFEMFGHRGIWADGWKAVAYHPPGTPFEDDTWELYDLDRDFAENHDLAAADPG
                     RLESLQAEWWRQAEDNKVLPLDDRFAERFAENAERHRGGRTHYELWAGLGHVPTDAAP
                     DLRSRSYTITVEILEPGDGVLVAHGDQTSGYSLHVADGHVVHDLSVGGHHQVVRADRP
                     LPDGPCTVTFRLERGEPFLGRAPGTGTLAIDGEVCGRFETDRIFVVTISWSGLDVGRD
                     RASPVGDYESPNAFTGRIRRVVFDLDDDQDLDHRAVAEAESARE"
     gene            complement(6001..6156)
                     /locus_tag="VLR27_04570"
     CDS             complement(6001..6156)
                     /locus_tag="VLR27_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6131 family protein"
                     /protein_id="HSP02750.1"
                     /translation="MIVLGLILLLVGLLADIGILVTLGVILAVVGAILALLGSTGRAV
                     GGRNHWF"
     gene            6247..7014
                     /locus_tag="VLR27_04575"
     CDS             6247..7014
                     /locus_tag="VLR27_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02751.1"
                     /translation="MYPAVDDRLARDVLAAVGARGDDDVEAVVRAIRAWLPAGSTAKW
                     VAADAGRVPPGADPAEALEARLGGSLESWSCWAFCTSVGALLAGAGHDVNIVVEHHRT
                     ASVVDYHSSLIVDGELLDPYLGPSAPVPLGHDVTRRDAWVSWVPADVPGGRWDHLGLR
                     GGGAPYRYRVLGEHLDRQDVAAFCEISATHSGVGRRRNAHWVTDDRLWFVREGDDGTA
                     ELRVAEGPTPFEQHRRVVARGPYEELRAAIDGTEHSR"
     gene            7011..8294
                     /locus_tag="VLR27_04580"
     CDS             7011..8294
                     /locus_tag="VLR27_04580"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HSP02752.1"
                     /translation="MTTALAPPVPDLELRQRDIRRAKRRATGLLVLVTVAFLVVAITT
                     DREGGTGYLRAGLEAAMVGGLADWFAVVAIFRHPLGIPIPHTAVIVARKDQFGQTLGQ
                     FVQEHFLNAPTIGERVRSMGVADRASSWVLERDAGGVPVNAERLAGHAAELVVRLAEA
                     VEDDDLVHTVETAVRTRLADVDVAPLGARLLTVVTEEGRHRELVDAVLVGLDGFLAEN
                     EVLLRQRFASESPWWVPDVVDDRIFERLLTGVRAIIRGDTVGGSDELRSRIDVAVARI
                     IGRLEHDEVWQDRAEQLKQELLDHPALRSFLDSLWADTKATLRTQALDGGSALRQRLT
                     ELIVSAAERFRADERAMARADELAEQAARAVVDTFGDEITRLISATISQWDGEETASR
                     LELLLGRDLQFIRINGTIVGGLAGLVIHAVGELAG"
     gene            complement(8349..9314)
                     /gene="meaB"
                     /locus_tag="VLR27_04585"
     CDS             complement(8349..9314)
                     /gene="meaB"
                     /locus_tag="VLR27_04585"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HSP02753.1"
                     /translation="MTLADDVLAGDRRALGRAITLVESTRADHRADAVELLQAVLPHT
                     GTGIRLGVSGAPGVGKSTFIDAFGCHVIDQGTTLAVLAVDPSSGRSGGSILGDKTRME
                     ELARRPEAFIRPSPAGRTLGGVARRTREAMLLCEAAGYDAVLVETVGVGQSETAVAEM
                     VDLFCLLAGPGAGDDLQGVKRGVMELAELLVVTKADGDLEPAAKRASADLKHALQLLR
                     PVHHAWSPRVLLTSAHTGTGLAEVWDAVVEHRRALEDSGELQSLRGEQARQWLWTELR
                     EGLVERFVAHAGEAVRSAELDVIAGRELPTIAADRLLDHERPREL"
     gene            complement(9311..>10318)
                     /locus_tag="VLR27_04590"
     CDS             complement(9311..>10318)
                     /locus_tag="VLR27_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSP02754.1"
                     /translation="RNTQLILGDETDVTKVVDPLAGSYYVESLTAGLADAAWALIEEV
                     EGMGGMTKAIEAGMPKLRIEESAAKRQARVDRGEDVIVGVNKYVPENVEMVDVLDIDN
                     AEVRESQIRRLEQIRASRDQAACDAALLALKEGAAGDANLLQLCVDAARARATVGEMS
                     DAMEAVFGRHKAEVRSIHGVYGAAYADDEDYAALLARVDGFAEANGRRPRMLVAKMGQ
                     DGHDRGGKVIATAFADFGFDVDVGPLFQTPEEVARDAVEQDVHIVGVSSQAAGHKTLV
                     PQLIEELNKAGASDVLVVCGGVIPPQDYDFLRDAGVSAVFGPGTNILEAAGTVLDLLQ
                     G"
BASE COUNT         1360 a         3743 c         3880 g         1335 t
ORIGIN      
        1 aggatgccgc cgctcccgac cgtctcgggc agggcggtgg acgcgtaggc caccaccggg
       61 aggccgctgg ccatcgcctc gagcaccggg atgcagaagc cctcgtgctc ggaggcgcac
      121 acgaacacgt cggcttcggc gtactcccgg gcgaggcggg cgtcgtcgac gccgccgacg
      181 aggtgcacgg cgccctcgag gtcgagctcg gtgatcaggt cgcgcacggc gtcggtgtac
      241 gccggggccg agtcccgtcc gaccagcacc agttgcgcgt ccgggtcgtg gacggcacgg
      301 tgccaggcga gcgcgagcac gaggtcgtgc tgggccttgt tcggcaccac ccgaccgacg
      361 aacagccagc gcccggcccg ccgtcccgag cccgccccgt cgtggcgcag gccgcgcacg
      421 tcgaggagga tggggaccac ggcggtgcgg gcgaacccgg ccgcctccag ctcgcgggtg
      481 ttgaacgccg agtcgccgat gccgagcacg gcccgtcggg cgaggtcgcc cagctggctg
      541 cggccccagc cggccaggtg cgccatgtcc ggcgcccagc gcaggaggtg ctccaccggc
      601 gtgaggttgt ggtagtcgac gaccagcggc tcggggcggg cgaggaggcg atcgaccacc
      661 tcgttgccga tcgagcactg gtagagcagc gccagtccgg gcgtgcctgg ctcgggcagg
      721 gcgtcgaggg gccgggcctg gccggccagc gccgggtgga cgtcgtcgct gaagatctcc
      781 gacgccagcc cggccgatcg caggacgtcg cgcacggcca gggtgtgccc gcccacgcca
      841 tcgccggggg tcagcgacgg gacgacctgg tggatggtgt cgatcaccac cgggccgcca
      901 ccacgaacgc accgtcgccg cggtggacct ccacgtcgct gccacctcgc gcctcgagca
      961 ggtgggccca cgtctcggcg tgcaccggtc ccggctcgcc caggtcacgg gtgacggggt
     1021 cggcgagctc ggcccacgcg tcaggggcgg cggagaccac ggcgagccgg cccccggcgg
     1081 cgacgccgcg cacgagctgg tcgagcgcag ccaggcggga ggggagcggc ccgacgtcga
     1141 ggaggcggta ggcgacgacg agcccggcgt caccggccgg gagggtggcc agctcgacgg
     1201 tgctgccgac gtcgcggcgc acccccgagg cgcctgcctc gagcgcgccg agcgcatcga
     1261 cgagtgcggg ccgcacatcg ggcgcgccgg cgatgacggc gtcgaggccg tggacctcga
     1321 ggcggccgac ccgctcgtcg agcaggcgca ccgcggcgga cagcccgctc acgaccgcgg
     1381 tggtctgctg ggcgaggtgg cgggcgacga acgcgaccgc cttgcgcacg gaccacttga
     1441 gctgggggac caccggccgg gcggcgtcca cgggggcgtc gacgtcgatc acggcccggc
     1501 ggtcgacgtc gtcgagcagg ccccgcacgt cgtggcgggc cgatccgaac cggctgccgg
     1561 cggcggcgag gcgggcggcg agctcggggt gggcggcgcg ctcggcgtcg gcggcgccgg
     1621 cgacgtcgac gacggctcgc caggggtcgg gggactcagc catggcggct catactcgca
     1681 gagcgcccct cccagctccc ggatgagtcg agcaccgcgg cggtgcgcat gaggaacgag
     1741 gccatctggt cgcggcgcac gtccgcgccc ggtcggtacg tgccgttcgc gtacccggtg
     1801 gcgatgccgg ccccggcggc ccggtcgacg ttgtccccgt gcaccgggtc ccggtcgtgg
     1861 aaccaggatc gggtgagggg gagggtggtg ccggtgacgt ggcggtgggc gcgcacgagg
     1921 aaggtcgcca tctgcccgcg ggtcacggcc gcgccggggg cgtaggaggt ggcggtccgt
     1981 cccgcgacga cgtccaccgc ggcgagccga tcgatgttgt gctcgtgggg ctcgtacgcc
     2041 gccccgtcgt cgtcgccgaa ggcgtcgggg ggatcctcgg gcagcggcgc gcccgccgcg
     2101 tcgatgagcc gggcgacgaa cgacgccatc tgcgcccgcg tgaccgactg cgccggccgg
     2161 tacatcccgt cgacgggctg ggagatctcc caccacaccg cgcactccac cgcccggtgg
     2221 tgcggcgatg agggcgggac gtcgcggacg cccgaggacg gcacctcgtc ctccgggcac
     2281 accgggtcga tggggcgggg tgtcggtggc ggtccgagtg ggaacacccg ccggcgatcc
     2341 ggggtggcgc cgtacatggg ccagggcgtc tccgccggtg tggcgatgtc gaaggcctgg
     2401 atggtggcgg cgttgccgtt gccgggctgg aacccggcag cgacgagggc ccaccgcccc
     2461 cggccgagct cacccacggc ggctgcgttc ttgtacgccc cggcgcccag ggagcgcagg
     2521 gtcttcccgg tgtggccgtc gagcacgtgc acccggccgc cggaggcgac gatgacctcg
     2581 ttcccggggt cgggcagcac gtcggcgatg acggggggcg acgtgagctc gtccgaggcg
     2641 ggctcgacct cccacagcgg ggtgccgtcg ccccggaggg cgaggaccgc gccgcggccg
     2701 ggcaggaagc tgccgatcac cacgtcccgc tggcggtcgg cgttcacgtc gccgacggcg
     2761 ggcgcggcga ggaaggtgtt gtagcgagcg gtgcgcggcc aaccggggac gtcgcgcccg
     2821 gtcagcaggt cgaacgccca gaccttgttc gagtcgtggc agcgctgcgc ggactggcag
     2881 tagtagcggc cggcgccggt gaccacctcg aggcgaccgt cgccgttgat gtcggcgatg
     2941 gcgggggcgg tctggaacgc ctcgctgctg agccggaccc agcgctgctg cagctgcggt
     3001 ccgccggcgt agcgcagggc gcggaagaac ccaccggagt ggctgccgtg gttgctggtg
     3061 gcgtcgccgc cgatgtagat ctcgtcacgc ccgtcgccgt cggcgtcgaa cagggccggc
     3121 gccgaccaga ccgtgtcggt gttgtcgaag gcggcgacct ggcggcacgc tccgtcgagc
     3181 acccggatct ggtggtccca ggagccgatg acgatgtcga tgcgaccgtc gccggtcacg
     3241 tcccccaggg cgggggaggc ccacaccgcg tcgctgaacc cgtcgggccc accgccggac
     3301 cactggtcga acttgtcgcc ggtctggaag cggcaccggg tgctgccgtc gccctcgagc
     3361 acgacgacgc cgccgtgctg gtcggggacc tcgagcgagc cggtgccgac gaccacctcg
     3421 tcgcggccgt ccccgtccac gtcgccgacg gccggcgacg atgccaccgc gctccgggtg
     3481 cccgggacga tcgaggcgtt cgccggccag cccggcagcg ggcggccgtc cgcgtcgtag
     3541 gcgtggaccc gtccgtcgag accgcccacg acgacgtcgg atgccgcgtc gccgtccacg
     3601 tcggcgatgg cgggggagga ccagtggacc cgcccgccgg ggacggtgac gtcccaccgc
     3661 gacgacccgt cggcgacggt ggcctcgacg gacggtgcgg accacgccgc cgacgcgagt
     3721 gccagtgaga cgatggcggc cgccgctccc gatcgcatcg agccaggcta cgtgccgcag
     3781 acgacgtccc cgaacgggcc cataggttgt ggcgatgacc gtgacgggac gccggccgaa
     3841 cgtgctcgtg gtgctgttcg acgacgtggg gttctccgac ttcggctgct acggctccga
     3901 gatcgccacg cccacgatcg accgcttggc gtcggcgggc ctgcggtact ccggcttcca
     3961 caccaccgcg atgtgctcca ccacgcgcgc cgccctgctc accggccgca accaccacaa
     4021 cgtcggcgtc ggctgcctgg ccaacttcga ctcgggcctg cccggctacc ggggcaagat
     4081 cagccgggag gccgggacgg tggccgagct gctcgacccc cacggctacc gctgctacct
     4141 caccggcaag tggcacgtca cgccgctgac cgagaccggt ccgaccggtc cctacgacgg
     4201 ctggccgctg cagcggggct tccaccgctt ctacggcttc ctcgacgccg agaccgacca
     4261 gtacgcgccc gagctcgtcc acgacaacca ccacgtcgac ccaccggcca ccttcgagga
     4321 gggctaccac ctctccgccg acctggcgga caagacggtg gagttcctgc gcgaccaccg
     4381 ctacggcgtg cccgacgagc cgtggctcgc ctggctcgcg ttcggcgcct gccacgcgcc
     4441 ccaccaggcc ccgcgcgagc tcatcgacca ctacgagggg gtgttcgcca agggctggga
     4501 cgtcacgcgc caggggcgcc tcgagcgcca gatcgccctc ggcctggtcc cggccggcac
     4561 cgagctgccg ccccgcaacg accgggtgcg ggcctgggac gaccacagcg cggacgagca
     4621 gcgcgtgttc acccggttgc aggccgccta cgccgccatg ctcgaccacg ccgaccagca
     4681 gctggcgagg gtggtcgcgt tcctggagga gaccgaccag ctcgacgaca ccctcgtgct
     4741 cgtcctgtcg gacaatggag cgtcccagga gggcgggccg tcagggttcg tcaacgccat
     4801 ggggccgttc aacgcccagc ccgagccgct cgaggccaag ctcgccaccc tcgacgcggt
     4861 cggcgggccc gggacgcacg ccaacttccc gtggggctgg gcgatggcgg ccaacacgcc
     4921 gctgaagcgc tacaagcaga acacccacgg cggcggcatc cgggacccgc tggtggtgtc
     4981 ctggccggtg ggcatcggtt cgcgcggtga ggtccgccac cagttctgcc acgccgtcga
     5041 cctcgtgccg acgatcctcg acgtcctcgg caccgagccg ccggcagcca tcgccggggt
     5101 gacccagctg cccatggacg gcgcctccat gcggtcgacc ttcgacgacg ccgacgcgcc
     5161 cccgccgaag gacgtgcagt acttcgagat gttcggtcac cgcggcatct gggccgacgg
     5221 gtggaaggcg gtggcgtacc acccgccggg cacgcccttc gaggacgaca cctgggagct
     5281 gtacgacctc gaccgggact tcgccgagaa ccacgacctg gccgcggccg atccgggccg
     5341 gctggagtcg ctgcaggccg agtggtggcg ccaggccgag gacaacaagg tcctgcccct
     5401 cgacgatcgc ttcgccgagc gcttcgccga gaacgccgag cgccaccgag gcggtcggac
     5461 gcactacgag ctgtgggccg gcctcggcca cgtgccgacc gatgcggcgc cggacctgcg
     5521 cagccgcagc tacacgatca ccgtcgagat cctcgaaccg ggcgacggcg tgctcgtcgc
     5581 gcacggcgac cagacgtcgg ggtactcgct gcacgtggcc gacggccacg tcgtgcacga
     5641 cctctcggtc ggcgggcacc accaggtggt gcgggccgac cggccgctgc cggacggtcc
     5701 gtgcacggtg acgttccggc tggagcgggg ggagccgttc ctgggccgtg cccccggcac
     5761 cggcacgctg gccatcgacg gcgaggtgtg cgggcgcttc gagaccgacc ggatcttcgt
     5821 ggtcacgatc tcgtggagcg gtctcgacgt gggccgtgac cgggcatcgc ccgtgggcga
     5881 ctacgagtcg ccgaacgcgt tcaccgggcg gatccgccgg gtggtcttcg acctcgacga
     5941 cgaccaggac ctcgaccacc gtgcggtggc cgaggccgag tcagcgaggg agtagcgaat
     6001 tcagaaccag tggttgcgac cgcccacggc ccggccggtg gagccgagca gggcgaggat
     6061 ggcgccgacc acggcgagga tgacgccgag ggtgacgagg atgccgatgt cggccaggag
     6121 gccgacgagg agcaggatga gtccgagtac gatcatggcg gcaacgtacc ccggtggggg
     6181 atgtgtcaga acggatgcct tgagcgggct cgtccgaccc gccgagggat cggcatgctg
     6241 tcgggcgtgt atcccgccgt cgacgaccgc ctcgcccgcg acgtgctcgc cgcagtcggc
     6301 gcccgcggcg acgacgacgt ggaggccgtc gtgcgggcga tccgggcctg gttgccggcc
     6361 gggtccaccg ccaagtgggt cgccgcggac gccggtcggg tgccacccgg ggccgacccg
     6421 gccgaggcac tcgaggcccg cctcggcgga tcgctcgagt cgtggtcgtg ctgggccttc
     6481 tgcaccagcg tcggcgcgct cctggccggc gccggccacg acgtcaacat cgtcgtcgag
     6541 caccaccgca ccgcctcggt ggtcgactac cacagctcgc tcatcgtcga cggcgagctg
     6601 ctcgacccgt acctcgggcc atcggcaccc gtcccgctcg gccacgacgt gacccgacgc
     6661 gacgcgtggg tgtcgtgggt gcccgcagac gttcccgggg gccgctggga ccacctcggg
     6721 ttgcgaggcg gcggcgcccc ctaccgctac cgggtgctgg gcgagcacct cgaccgccag
     6781 gacgtcgctg cgttctgcga gatctcggcc acccattcgg gggtcggccg ccggcgcaac
     6841 gcccactggg tcaccgacga ccggctctgg ttcgtgcggg agggcgacga cggcaccgca
     6901 gagctgcggg tcgccgaggg gccgacgccg ttcgagcagc accggcgggt ggtcgcccgg
     6961 ggtccgtacg aggagctgcg cgccgccatc gacggaacgg agcacagccg atgacgaccg
     7021 ccctcgcccc gcccgtgccg gacctggagc tgcgccagcg ggacatccgt cgggccaagc
     7081 ggcgcgccac ggggctgctg gtgctggtca ccgtcgcctt cctcgtcgtc gccatcacca
     7141 ccgatcggga gggcggcacc ggctacctgc gggcgggcct ggaggcagcg atggtcggcg
     7201 gcctcgccga ctggttcgcc gtggtcgcca tcttccgcca ccccctcggc atcccgatcc
     7261 cgcacaccgc ggtcatcgtc gcccgcaagg accagttcgg ccagacgctc gggcagttcg
     7321 tgcaggagca cttcctgaac gcccccacca tcggcgagcg ggtccggagc atgggggtcg
     7381 ccgaccgggc ctcgtcgtgg gtcctcgagc gcgacgccgg cggtgtgccc gtcaacgccg
     7441 agcgcctggc cggccacgcc gccgagctgg tggtccgcct ggccgaggcg gtcgaggacg
     7501 acgacctggt gcacacggtg gagaccgcag tgcgcacccg acttgccgac gtggacgtgg
     7561 ccccgctcgg ggctcgcctg ctgacggtcg tcaccgagga ggggcgccac cgggagctgg
     7621 tcgacgcggt gctcgtcggc ctcgacggct tcctcgccga gaacgaggtg ctcctgcgcc
     7681 agcgcttcgc gtcggagtcg ccgtggtggg tgcccgacgt ggtcgacgac cggatcttcg
     7741 agcgcctgct caccggtgtc cgggccatca tccggggcga caccgtgggt ggctcggatg
     7801 agctgcgcag ccgcatcgac gtggccgtgg cccgcatcat cgggcgcctc gagcacgacg
     7861 aggtctggca ggaccgggcc gagcagctca agcaggagct gctcgaccac ccggccctgc
     7921 gcagcttcct cgactccctg tgggccgaca ccaaggccac cctgcggacc caggcgctcg
     7981 acggcggctc ggcgctgcgt cagcgcctca ccgagctcat cgtctccgcg gccgagcgct
     8041 tccgggccga cgagcgggcg atggctcgcg ccgacgagct ggccgagcag gcggcccggg
     8101 cggtggtcga caccttcggc gacgagatca cccgcctcat ctcggccacc atcagccagt
     8161 gggacggtga ggagaccgcc tcccgcctcg agctgctgct cggccgcgac ctgcagttca
     8221 tccgcatcaa cggcacgatc gtgggcggcc tcgccggcct ggtgatccac gccgtcggcg
     8281 agctcgccgg ctgagccgtt cgtcgctcct gacggagctc ctcacttctg gagttgctcg
     8341 gctgcgcctc acaactcccg cggtcgctcg tgatcgagga ggcggtcggc ggcgatggtg
     8401 ggcagctcgc gtccggcgat cacatccagc tcggcgctgc gcaccgcctc gccggcgtgg
     8461 gcgacgaagc gctcgacgag cccctcgcgg agctcggtcc agagccactg cctggcctgc
     8521 tcgccccgca gggactgcag ctcgccggag tcctcgaggg ctcggcggtg ctcgaccacg
     8581 gcgtcccaca cctcggccag gccggtgccg gtgtgggcgg aggtgagcag cacccggggg
     8641 gaccaggcgt ggtggacggg gcgcagcagc tggagggcgt gcttcaggtc ggcggacgcc
     8701 cgcttcgccg ccggttcgag gtcgccgtcg gccttggtga ccaccaacag ctcggccagc
     8761 tccatgacgc cgcgcttgac gccctggagg tcgtcgcccg cccccggccc ggccagcagg
     8821 cagaacaggt cgaccatctc ggcgaccgcg gtctcggact ggccgacccc gacggtctcc
     8881 accaggacgg cgtcgtagcc ggcggcctcg cacagcagca tcgcctcgcg ggtgcggcgg
     8941 gcgacgccgc cgagcgtgcg cccggcgggg gaggggcgga tgaaggcctc gggtcgccgg
     9001 gccagctcct ccatccgggt cttgtcgccg aggatcgagc cgcccgagcg gccggatgac
     9061 gggtcgacgg cgagcacggc cagcgtcgtg ccctggtcga tgacgtggca gccgaacgcg
     9121 tcgatgaacg tggacttgcc gacgccgggc gcgcccgaca cgccgaggcg gatgccggtg
     9181 ccggtgtggg gcaggacggc ctgcagcagc tcgaccgcgt cggcccggtg gtcggcgcgc
     9241 gtggactcga ccagcgtgat ggcccggccg agggcccgcc ggtcgccggc gaggacgtcg
     9301 tcggcgaggg tcacccctgg aggaggtcca ggaccgtgcc ggctgcctcg aggatgttcg
     9361 tgcccggccc gaacacggcg gagacgccgg cgtcgcgcag gaagtcgtag tcctggggcg
     9421 ggatcacgcc gccgcacacg accagcacgt cgctggcgcc ggccttgttc agctcctcga
     9481 tgagctgggg cacgagcgtc ttgtgcccgg ccgcctggga cgacacgccc acgatgtgca
     9541 cgtcctgctc gacggcgtcg cgggcgacct cctcgggcgt ctggaacagc gggccgacgt
     9601 cgacgtcgaa gccgaagtcg gcgaacgcgg tggcgatgac cttgccgccg cggtcgtggc
     9661 cgtcctggcc catcttggcg acgagcatgc gcgggcgacg gccgttggcc tcggcgaacc
     9721 cgtcgacgcg ggcgagcagg gcggcgtagt cctcgtcgtc ggcgtaggcg gcgccgtaca
     9781 cgccgtggat gctacggacc tcggccttgt ggcgaccgaa gacggcctcc atggcgtcgc
     9841 tcatctcgcc gacggtggcc cgggcccgag ccgcgtccac gcacagctgg agcaggttgg
     9901 cgtcgccggc cgccccctcc ttgagcgcga gcagggcggc gtcgcacgcg gcctggtcgc
     9961 ggctggcccg gatctgctcg aggcgtcgga tctgggactc gcgcacctcg gcgttgtcga
    10021 tgtcgagcac gtcgaccatc tcgacgttct ccggcacgta cttgttgacg ccgacgatga
    10081 cgtcctcgcc ccggtcgacc cgggcctggc gcttggccgc cgactcctcg atgcgcagct
    10141 tgggcatgcc ggcctcgatg gccttggtca tgccgcccat gccctcgacc tcctcgatga
    10201 gcgcccacgc cgcgtcggcg aggccggcgg tgagggactc gacgtagtac gagccggcga
    10261 gggggtcgac gaccttggtc acgtcggtct cgtcgccgag gatcagctgg gtgttgcg
//
LOCUS       DATISU010000108         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_843668, whole genome shotgun sequence.
ACCESSION   DATISU010000108 DATISU010000000
VERSION     DATISU010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843668"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..788)
                     /locus_tag="VLR27_04595"
     CDS             complement(186..788)
                     /locus_tag="VLR27_04595"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HSP02755.1"
                     /translation="MVAKRVLDVVVAGAALVLLSPVLAVVALLVRLRLGSPVVFRQER
                     AGLRGQPFVLTKLRTMTDERGADGELLPDEDRLPAFGSFLRSTSLDELPELVHVLRGE
                     MSLVGPRPLPTKYLARYSPRQTRRHEVRPGITGWAQVNGRNAAGWDDRLEMDVWYVEN
                     RSLLLDLKILWRTLRVVTSREGVAEDGRATMTEFLGTDEP"
     gene            complement(801..1985)
                     /locus_tag="VLR27_04600"
     CDS             complement(801..1985)
                     /locus_tag="VLR27_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02756.1"
                     /translation="MKLLLQVAMAVFVWTVVIGILNGADVVDFDRMVLLSHVHAGTLG
                     WITTSVVAASLWLFGATGSPGEVRLGRGLAWATVVVLPVFALTFAFTTEDPRAILGGL
                     ALLVIMSVFAWVVARTRRIEVSTVHLGFLVAVATSVVGGIIGLLVATEMATGRDVVPD
                     GGYDAHPATMVVGFLIPVGMALAEWGLRGTASGRAGRLGTAQMGLPFVGGLLLMVGLL
                     LDIDALPPLATLIELVGVGIFFKRMWPDLRAVAWGERTPGRYAAASAIAIIVNIAFLN
                     YLVGANGGDIDLVGMHQILAMDHTMFIGVLTNAIFALLAVATAGRTRWPRLDGVVFVG
                     MNAGLVVFVISLLAESTWMMRAATPVLGLAILAGLLDRTLALQGATARPDVGMTPAPA
                     AT"
     gene            complement(2104..>2663)
                     /locus_tag="VLR27_04605"
     CDS             complement(2104..>2663)
                     /locus_tag="VLR27_04605"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSP02757.1"
                     /translation="TFDDGTDDFVDEALPVLERHGVPATYYLATEFIDRQQPFPDGGV
                     ALTWDAAKEMVASGLVDIGSHTHTHALLDRIPPTEAAHELDRSVAVIETHLGVTPEHF
                     AYPKALLGTPQNEFEVRKRFRTATIGRTRPNPWEGSDLHRLTRSPIQVSDGMDFFERK
                     VRGGMRLENDVRDLVNRWRYRGATR"
BASE COUNT          432 a          933 c          941 g          357 t
ORIGIN      
        1 gtctggccgt agccgagcct gctcaccgtg tccgtgatga ccatcgtgca cccccttcgg
       61 gcgatcgacc tggcgtggtc gctaccccct ctgacgtgcg cgatgaccgg atggatcccg
      121 ttgccaccaa cctgcaacac aggcgcaacc tggcgtggaa ctggcagcgg tggcggaaca
      181 gccggtcagg gctcgtcggt cccgaggaac tcggtcatgg tcgcccgccc atcctcggct
      241 acgccctctc gggacgtgac gacccgcagc gtgcgccaca ggatcttcag atcgaggagc
      301 aacgagcggt tctcgacgta ccagacgtcc atctcgaggc ggtcgtccca gccggcagcg
      361 ttgcggccgt tcacctgggc ccagccggtg atgccgggcc ggacctcgtg gcggcgggtc
      421 tggcggggcg agtaccgcgc caggtacttc gtcggcaggg ggcggggacc gaccaggctc
      481 atctcaccgc gcaggacgtg cacgagctcg ggcagctcgt cgagcgacgt cgatcggagg
      541 aacgatccga acgccggaag acggtcctcg tccggcagca gctcgccgtc ggcgccacgc
      601 tcgtcggtca tggtccggag cttggtgagc acgaacggct gcccacgaag tccggcccgc
      661 tcttggcgga acacgaccgg cgacccgagc cgcagtcgca cgaggagcgc cacgaccgcc
      721 agcaccggcg aaagcagcac gagggcagcg ccggcgacga cgacatcgag cacccgcttg
      781 gcgaccacgg gtacgggcta tcaggtggcg gcgggggcgg gggtcatccc gacgtcgggg
      841 cgggcggtcg cgccctgcag ggcgagcgtg cggtcgagca ggccggcgag gatggccaga
      901 ccgagcacgg gggtggccgc ccgcatcatc caggtcgact cggccagcag cgagatgacg
      961 aagaccacca ggccggcgtt catgccgacg aacacgacgc catcgaggcg tggccagcgg
     1021 gtccgaccgg ccgtcgccac cgccagcagg gcgaagatcg cgttggtgag cacaccgatg
     1081 aacatcgtgt ggtccatggc gaggatctgg tgcatgccga cgaggtcgat gtctcccccg
     1141 ttcgccccga ccaggtagtt gaggaacgcg atgttgacga tgatggcgat ggcggaggcc
     1201 gcggcgtagc gaccgggtgt gcgctcaccc cacgccaccg ctcgcaggtc tggccacatg
     1261 cgcttgaaga agatgccgac gccgacgagc tcgatcagcg tggccagcgg cgggagggcg
     1321 tcgatgtcca ggagcaggcc gaccatcagc agcagccccc cgacgaacgg cagccccatc
     1381 tgcgcggtgc ccagtcgtcc ggcccgcccg ctcgcggtac cccgtagccc ccactcggcc
     1441 agtgccatgc cgaccgggat gaggaagccg accaccatcg tcgccgggtg tgcgtcgtag
     1501 ccgccgtcgg gcacgacgtc gcgaccggtc gccatctcgg tggcgacgag cagaccgatg
     1561 atgccgccca ccaccgacgt ggccacggcg acgaggaacc cgaggtgcac ggtgctgacc
     1621 tcgatgcgtc gggtccgggc cacgacccag gcgaacaccg acatgatcac cagcagcgcc
     1681 agtccgccca ggatcgcccg ggggtcctcg gtcgtgaacg cgaacgtcag ggcgaacacc
     1741 ggcagcacga ccacggtggc ccaggccaac cctcgaccga ggcggacctc gccggggctg
     1801 cccgtcgctc cgaacagcca cagcgatgcg gccacgacgc tggtcgtgat ccagccgagc
     1861 gtgccggcgt gcacgtgcga cagcaggacc atccggtcga agtcgacgac gtcggcgccg
     1921 ttcaggatcc cgatgaccac ggtccacacg aacacggcca tggccacctg caggagcagc
     1981 ttcacctccc cccggagtgg atgctgcccg tcgacgaccg cgtgcgcgtc catcactgcc
     2041 ctccccctgg gcctcccccc tcgagcgacc ctcgccggat ggtacgcccg tccggccgat
     2101 ctgtcagcgg gtcgcgcccc ggtaccgcca gcggttcacc aggtcgcgca cgtcgttctc
     2161 gagccgcatg ccgccccgaa ccttgcgctc gaagaagtcc atgccgtcgg acacctggat
     2221 cggcgaacgg gtgaggcggt gcaggtccga cccctcccac gggttcggcc gggtgcgacc
     2281 gatggtggcg gtgcggaacc gcttccggac ctcgaactcg ttctgcggcg tcccgagcag
     2341 cgccttcggg taggcgaagt gctcgggcgt gaccccgaga tgcgtctcga tgacggcgac
     2401 ggatcggtcg agctcgtggg cggcctcggt cggcgggatg cggtcgagca gggcgtgggt
     2461 gtgggtgtgg gagccgatgt cgacgaggcc ggacgcgacc atctccttcg ctgcgtccca
     2521 cgtcagagcg acaccgccgt cggggaacgg ctgctgccgg tcgatgaact cggtggccag
     2581 gtagtaggtg gccggcacgc cgtgacgctc gaggacgggc agcgcctcgt ccacgaagtc
     2641 gtccgtgccg tcgtcgaagg tga
//
LOCUS       DATISU010000109         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_470964, whole genome shotgun sequence.
ACCESSION   DATISU010000109 DATISU010000000
VERSION     DATISU010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470964"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2164
                     /locus_tag="VLR27_04610"
     CDS             <1..>2164
                     /locus_tag="VLR27_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HSP02758.1"
                     /translation="RLAAQGRMVPRLRKVRRRDDAGGKDLSTFEVHWEPVLIDPEAIN
                     ELAAARPTAAAATGNRQDGRAFTQSVLSDLLGTICEMAAERLEVPAPPPDPRSRSDVA
                     ETVLARLDGKPFDAPTRAGAELVRRLDQWSSPVTGTAKLALTVRLDPPDEGNAWHCAV
                     LAPGNDDVLEPVEVAMVNASNTKSREMKAQLARVEKLFPELSRPGGRRRGEVILSQEE
                     AWQLMSQTGDVLRAAGFDVQVPLLSRRKPAPGLRLTADEAQESVVGAQQLANVRWSVV
                     FDGVELSAEDVRRLATQARPLIKSGDKWVEVDKADLREAAAALAERADKTRLTGADML
                     RQALGLEGSPLAGGIQITGAGWAADLLRSASQISEDTVSATPEGFDGELRHYQAEAVA
                     WLRFLDDAGLGGCLALDMGLGKTPTMLAHVAATAEKGPTLVVAPPAVVGNWANEARKF
                     TKRLRVVVHHGPNRTSSRGIPKMVENADVVITTYGTAVRDVAALEKVEWGKVVLDEAQ
                     AIKNPTSDTAQQLRRIEARSRVALTGTPIENGLGDLWSIIDYCNPGLVGGRAQFIAQL
                     SPSGTGKDGAESALKALNGILVFRRTKAEPAIAAELPDRIDELDHCAMTKEQIGLYQA
                     VLDRLVIDTSSEDSPQKKGAVLAAITALKQICNHPEAYQPTGEPLDGRSGKLTRLNEI
                     VESVFESGERVLIFTHFATWGDKLAKYLTDRYDLPIACY"
BASE COUNT          333 a          862 c          707 g          262 t
ORIGIN      
        1 gcgcctcgcc gctcagggcc gcatggtgcc ccggctgcgc aaggtgcgcc ggcgcgacga
       61 cgccggcggc aaggacctct ccaccttcga ggtccactgg gagccggtgc tcatcgaccc
      121 cgaggccatc aacgagctcg ccgccgcccg gcccaccgct gcggccgcca ccggcaaccg
      181 ccaggacggc cgagccttca cccagtccgt gctgtcggac ctgctcggca cgatctgcga
      241 gatggccgcc gaacggctcg aggtcccggc gccgccgccc gacccccgct cccggagcga
      301 cgtggccgag accgtgctgg cccggctcga cggcaagccc ttcgacgcac cgacccgcgc
      361 cggcgccgag ctggtccgcc gcctcgacca gtggtcgtca ccggtcaccg gcaccgccaa
      421 gctggcgctc accgtccgcc tcgacccgcc cgacgagggc aacgcctggc actgcgccgt
      481 gctggccccc ggcaacgacg acgtgctcga gcccgtcgag gtcgccatgg tcaacgcgtc
      541 caacaccaag tcgcgggaga tgaaggcgca gctggcccgc gtcgagaagc tgttccccga
      601 gctgagccgc ccgggtggcc gccgccgggg cgaggtcatc ctctcccagg aggaggcctg
      661 gcagctcatg tcccagaccg gcgacgtgct ccgcgccgcc ggcttcgacg tgcaggtccc
      721 gctgctgtcg cgccgcaagc ccgcgccggg tctgcgtctc accgccgatg aggcccagga
      781 gtccgtcgtc ggtgcccagc agctggccaa cgtgcgctgg tcggtcgtgt tcgacggggt
      841 cgagctgtcc gccgaggacg tccgccgcct cgccacccag gcccgcccgc tcatcaagtc
      901 cggcgacaag tgggtcgagg tcgacaaggc cgacctgcgc gaagccgcgg ccgcgctggc
      961 cgagcgggcc gacaagaccc gcctcaccgg cgccgacatg ctccgccagg ccctcgggct
     1021 ggagggctcg ccgctcgccg gcggcatcca gatcaccggc gccggctggg ccgccgacct
     1081 gctgcgcagc gcgtcgcaga tctccgagga caccgtctcg gccacccccg agggcttcga
     1141 cggcgagctg cgccactacc aggccgaagc cgtcgcctgg ctgcgcttcc tcgacgacgc
     1201 cggcctcggt ggctgcctcg ccctcgacat gggcctgggc aagacaccca ccatgctcgc
     1261 ccacgtcgcc gccaccgcgg agaagggccc gaccctcgtc gtcgccccgc ccgccgtcgt
     1321 cggcaactgg gccaacgagg cccgcaagtt caccaagcgc ctccgggtgg tggtccacca
     1381 cggaccgaac cgcaccagca gccgcggcat cccgaagatg gtcgagaacg ccgacgtggt
     1441 catcaccacc tacggcaccg ccgtccgcga cgtggccgcg ctcgagaagg tggagtgggg
     1501 caaggtcgtc ctcgacgagg cccaggccat caagaacccc acctccgaca ccgcccagca
     1561 gctgcgccgc atcgaggccc gcagccgggt ggccctcacc ggcaccccga tcgagaacgg
     1621 cctcggcgac ctgtggtcga tcatcgacta ctgcaacccg ggcctggtcg gcgggcgggc
     1681 ccagttcatc gcccagctgt cgccgagcgg caccggcaag gacggggccg aatcggccct
     1741 caaggcgctc aacggcatcc tcgtgttccg tcgcaccaag gccgaaccgg ccatcgccgc
     1801 cgagctgccc gaccgcatcg acgagctcga ccactgcgcc atgaccaagg agcagatcgg
     1861 tctgtaccag gccgtcctcg accgcctggt catcgacacc agcagcgagg actcgccgca
     1921 gaagaagggt gcggtgctcg ccgccatcac ggcgctcaag cagatctgca accaccccga
     1981 ggcgtaccag ccgacgggcg agcccctcga cggccggtcc gggaagctga cccgcctcaa
     2041 cgagatcgtg gagtcggtgt tcgagtccgg cgagcgggtg ctgatcttca cgcacttcgc
     2101 cacgtggggc gacaagctcg cgaagtacct caccgaccgc tacgacctgc ccatcgcctg
     2161 ctac
//
LOCUS       DATISU010000110         5613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_843695, whole genome shotgun sequence.
ACCESSION   DATISU010000110 DATISU010000000
VERSION     DATISU010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843695"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VLR27_04615"
     CDS             <1..1123
                     /locus_tag="VLR27_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HSP02759.1"
                     /translation="GLGVNGMNWATSPACTEVEMRMLDWFVELLGLPDHLRHDHDGPG
                     GGVLQDSASSATLVALLAARKRVTDGGDLSRLRAYTSEHAHSSVVKGARVAGLADDQV
                     RLVPADATHAMRADALAEAIEADVAAGLVPFFCCATAGTTSSMAFDPVPEIADICAAA
                     GIWCHVDAAMAGSAAVCPELRWVTDGADRVDSWSFNPHKWLFTTFDCTCLWFRDARPV
                     VDALSITPVYLRNEQSESGAVVDFRDWQVPLGRRFRALKPWLVMRHYGADGLARHIRE
                     HVRLGRLLADRVLDDPRFELAAPPALNLVVLRLTGDDADRRTTELLERVNASGEALLT
                     PTVLDGRPAIRICVGQTWTTQVHVDALWSLVDGLAGSPT"
     gene            1120..2079
                     /locus_tag="VLR27_04620"
     CDS             1120..2079
                     /locus_tag="VLR27_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP02760.1"
                     /translation="MTELDPADLDPARFEVHHFEGRGFRQAYVRVNPGGVPLLCVHGW
                     PETKRIWWRVIEPLAAAGFEVIVPDLRGFGDSDVAPDGFHDVASHSRDLHALVTSPGS
                     EGGLGHERVVLCGGDLGGPVIQDLALRFPEAVERMVLFNSPLPYDKEAMAGMDTRPRA
                     GAADYFLRQGTDPDGLAAELDTPEQRRSYIATFYTSRFWAHPGAFTSGQLPGGPAVDF
                     HTEPFAGAAKLRASFGGYESAFSEAARSEPALLARNPDVETLLLFGPSDHVLYPAFDE
                     MAAVTFPDHVGPFLLRDCGHFVPWEAPHQLVSATRAFCRDLLA"
     gene            2138..3277
                     /locus_tag="VLR27_04625"
     CDS             2138..3277
                     /locus_tag="VLR27_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HSP02761.1"
                     /translation="MGEIEIGLGKAGRRGYGLDELTIVPSRRTRDAELVDLSWELQGY
                     RFELPVLAAPCDSVVSPANAAGFDELGGLAVLDLEGLWTRYADPTAAFDEIADLPDDT
                     ASARLRELYQQPVDPDLVVERIRAVRASGAVAAGSVTPQRIESLLPRLVEAELDMLVI
                     RSTVLSAEHVTKTSADGGEPLNLKTFIRQLDVPVLVGSCTSYQAALHLMRTGAAAVLV
                     GVGTTVDATTADTFGVGVPLATAIADARAARMRHLDETGVYVHLIADGGMHTGGDVAR
                     AIACGADAVMLGAPLAGATDAPGRGRHWAPVAARAEVPHSACVRIDQRGTQREVLVGP
                     SDSSDGRLDLMGSLRSAMALSGYESLKEFQKAELIVSPGDRRGQA"
     gene            3274..4848
                     /gene="guaA"
                     /locus_tag="VLR27_04630"
     CDS             3274..4848
                     /gene="guaA"
                     /locus_tag="VLR27_04630"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HSP02762.1"
                     /translation="MTTPSIGTEFDTVLVVDFGAQYAQLIARRVREAHVHSEIVPHDI
                     TAAEIRERAPQGIIFSGGPASVHADGAPTIDQEIYDTGVPILGICYGGQLLARDLGGE
                     VAKTGRGEYGRADLECADLGTDGRSLLFGDDAPDQQVVWMSHFDTITQAPEGFRVVAS
                     TPDTPAAAFESTERGLYGVQFHPEVVHTQMGQDLLKHFLYDACGCRPSWTMLSVIETQ
                     VEAIRAQVGDGRAICGLSGGVDSAVAAALVHKAIGHQLTCVFVDTGLMRKDEGDQVVE
                     TFKRNFGIELIHVRAADRFFDKLAGVTDPEDKRKAIGELFIRIFEDAKGGITDADFLV
                     QGTLYPDVIESGGGAKAATIKSHHNVGGLPEDMDFDLVEPLRDLFKDEVRRVGEELGL
                     PEEIVWRQPFPGPGLGVRIIGEVTPDKVELLQEADAIVRAELHKAGMDREIWQAFAVL
                     PDIKAVGVMGDERTYGYPCIIRAVTSDDAMTADWARLPYDLLEKMSSRIINEVPGINR
                     VAYDITSKPPGTIEWE"
     gene            5028..>5613
                     /locus_tag="VLR27_04635"
     CDS             5028..>5613
                     /locus_tag="VLR27_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02763.1"
                     /translation="MLNARSRFRRTAPAAALLGAFALLTVTASPAAADPIGAGEASAF
                     GGQVQLTDQDVLPPTALAESTLADEDAVASALEVPADPLAISGTLNASAKVHADSDIA
                     SVLTQVPQTVEGPYNAAGVSSIEDLTVLVEQLPEGASLVSADVLRAEAAAVCRGGAVE
                     YTASSEIIDLQIGGEDVPLNDPLTQVVDGLNDLLE"
BASE COUNT          770 a         2132 c         1902 g          809 t
ORIGIN      
        1 gggcctcggc gtgaacggca tgaactgggc gacgagcccg gcgtgcaccg aggtcgagat
       61 gcggatgctc gactggttcg tcgagctgct cgggctgccc gaccacctgc gccacgacca
      121 cgacggaccc ggcggcggcg tgctgcagga ctcggcctcg tccgcgaccc tcgtcgcgct
      181 gctcgcggcc cgcaagcggg tcaccgacgg aggcgacctc tcacggctgc gggcctacac
      241 gtcggagcac gcccactcgt cggtggtgaa gggggcgcgc gtggcggggc tggccgacga
      301 ccaggtgcgc ctggtccccg ccgacgccac ccatgccatg cgggccgacg cgctggccga
      361 ggcgatcgag gcggacgtgg cggccgggct ggtgccgttc ttctgctgcg cgacggccgg
      421 cacgacgtcg tcgatggcgt tcgacccggt gcccgagatc gccgacatct gcgccgcggc
      481 gggcatctgg tgccacgtcg acgccgccat ggcgggatca gcagcggtgt gccccgagct
      541 gcgctgggtg accgacggtg ccgaccgggt cgactcctgg tcgttcaacc cgcacaagtg
      601 gctcttcacc accttcgact gcacctgcct gtggttccgg gacgcccggc cggtggtcga
      661 cgcgctctcg atcacgccgg tgtacctgcg caacgagcag agcgagtccg gcgccgtcgt
      721 cgacttccgg gactggcagg tcccgctcgg gcggcgcttc cgggcgctca agccgtggtt
      781 ggtgatgcgc cactacggcg ccgacggcct cgcccgccac atccgcgagc acgtccggct
      841 cggccgcctc ctggccgacc gggtgctgga cgacccccgc ttcgagctgg cggcgcctcc
      901 cgccctcaac ctcgtggtcc tgcgcctgac cggcgacgac gccgaccggc gcacgaccga
      961 gctgctggag cgggtgaacg catcggggga ggcgctgctc accccgaccg tgctcgacgg
     1021 ccgcccggcg atccgcatct gcgtcggcca gacgtggacc acccaggtcc acgtcgatgc
     1081 gctctggtcg ctggtcgatg gcctggcagg ctcaccgacg tgaccgagct cgaccccgcc
     1141 gacctggatc ccgcccgctt cgaggtccac cacttcgagg gccgggggtt ccgccaggcc
     1201 tacgtgcggg tgaaccccgg cggcgtcccc ctgctgtgcg tgcacggctg gcccgagacg
     1261 aagcggatct ggtggcgggt catcgaaccg ctggccgcgg ccggcttcga ggtcatcgtc
     1321 ccggacctcc gcggcttcgg cgactccgac gtggcgccgg acggcttcca cgacgtggcc
     1381 agccacagcc gggacctcca cgccctcgtc acgtcgcccg gatcggaggg gggcctgggc
     1441 cacgagcggg tggtgctgtg cgggggcgac ctcggcgggc cggtgatcca ggacctcgcc
     1501 ctgcgcttcc ccgaggcggt ggagcgcatg gtgctgttca actcgccgct gccctacgac
     1561 aaggaggcga tggccgggat ggacacccgt ccccgcgccg gcgccgccga ctacttcctg
     1621 cgccagggca ccgaccccga cggcctcgcc gccgagctgg acacgcccga gcagcggcgg
     1681 tcctacatcg ccaccttcta cacgtcgcgg ttctgggccc accccggcgc cttcaccagc
     1741 ggccagctgc ccgggggccc ggccgtggac ttccacaccg agcccttcgc cggcgcggcc
     1801 aagctgcggg cgtcgttcgg cggctacgag tcggccttct ccgaggccgc ccggtccgag
     1861 cccgccctgc tggcccgcaa ccccgacgtc gagaccctgc tcctcttcgg cccgagcgac
     1921 cacgtgctct acccggcgtt cgacgagatg gcggccgtca ccttccccga ccacgtgggt
     1981 ccgttcctgc tgcgcgactg cggccacttc gtgccgtggg aggcgccgca ccagctggtc
     2041 tcggccacgc gggcgttctg tcgcgatctg ctggcctgag aggccacctc actacactcg
     2101 cccctttccc tgaaaccgcc gacaaggggc gcaccacgtg ggtgagatcg agatcgggct
     2161 cggcaaggcc ggccgtcgtg ggtacgggct cgacgagctg accatcgtgc cgagccgccg
     2221 gacgcgcgac gccgagctgg tcgacctgtc ctgggagctg cagggctacc gcttcgagct
     2281 gcccgtgctg gcggctccgt gcgactccgt cgtcagcccg gccaacgccg ccggcttcga
     2341 cgagctgggc ggcctcgccg tgctggacct cgagggcctg tggacccgct acgccgatcc
     2401 caccgccgcc ttcgacgaga tcgccgacct gcccgacgac accgcctcgg cccggctacg
     2461 cgagctgtac cagcaaccgg tcgaccccga tctcgtggtc gagcgcatcc gggccgtgcg
     2521 ggcctccggc gccgtcgccg ccgggtcggt gaccccgcag cgcatcgagt ccctgctgcc
     2581 ccgcctggtc gaggccgagc tggacatgct cgtcatccgc tccacggtgc tgtccgccga
     2641 gcacgtcacg aagacctccg ccgacggcgg cgagccgctc aacctcaaga ccttcatccg
     2701 ccagctcgac gtgcccgtgc tcgtcggctc gtgcaccagc taccaggccg ccctgcacct
     2761 gatgcgcacc ggtgccgccg ccgtgctcgt cggggtcggc accaccgtcg acgccaccac
     2821 ggccgacacc ttcggcgtcg gtgtgcccct ggccactgcc atcgccgacg cccgtgcggc
     2881 gcgcatgcgc cacctcgacg agaccggcgt ctacgtgcac ctcatcgccg acggcggcat
     2941 gcacaccggc ggcgacgtcg cccgggccat cgcctgcggc gccgacgccg tcatgctcgg
     3001 cgcccccctc gccggtgcca ccgacgcgcc gggccgtggt cgccactggg cgcccgtggc
     3061 ggcgcgagcc gaggtcccgc actcggcctg cgtgcgcatc gaccagcgcg gcacccagcg
     3121 cgaggtgctg gtcggcccgt cggactccag cgacggccgt ctcgacctga tggggtcgct
     3181 ccggtccgcc atggcgctct ccggctacga gagcctcaag gagttccaga aggccgagct
     3241 catcgtgtcg ccgggcgatc gccggggtca ggcatgacca cgccctccat cggcaccgag
     3301 ttcgacaccg tcctggtcgt cgacttcggc gcccagtacg cccagctgat cgcccggcgc
     3361 gtgcgcgagg cgcacgtgca ctccgagatc gtcccgcacg acatcacggc agccgagatc
     3421 cgcgagcgcg ccccgcaggg gatcatcttc tccggcgggc ccgcctccgt ccacgccgac
     3481 ggtgccccga cgatcgacca ggagatctac gacaccggcg tgccgatcct gggcatctgc
     3541 tacggcggcc agctcctggc ccgcgacctc ggcggcgagg tggcgaagac cggtcgcggc
     3601 gagtacggcc gcgccgacct cgagtgcgcc gacctcggca ccgacggtcg gtcgctcctc
     3661 ttcggcgacg atgcccccga ccagcaggtc gtgtggatga gccacttcga caccatcacc
     3721 caggctcctg agggcttccg ggtggtcgcg tcgacgccgg acaccccggc tgcggccttc
     3781 gagtccaccg agcgcggcct gtacggcgtc cagttccatc ccgaggtggt ccacacccag
     3841 atgggccagg acctgctcaa gcacttcctg tacgacgcct gcgggtgccg tcccagctgg
     3901 acgatgctct cggtcatcga gacccaggtc gaggcgatcc gcgcccaggt cggcgacggg
     3961 cgggccatct gcggcctttc cgggggcgtg gactccgcgg tggccgccgc gctcgtccac
     4021 aaagcgatcg gccaccagct cacctgcgtc ttcgtcgaca cgggcctcat gcgcaaggac
     4081 gagggcgacc aggtggtcga gacgttcaag cgcaacttcg gcatcgagct gatccacgtg
     4141 cgtgccgccg accgcttctt cgacaagctc gccggcgtca ccgaccccga ggacaagcgc
     4201 aaggccatcg gcgagctgtt catccgcatc ttcgaggacg ccaagggcgg catcaccgac
     4261 gccgacttcc tggtccaggg cacgctctac cccgacgtca tcgagtccgg gggcggggcc
     4321 aaggccgcga ccatcaagag ccaccacaac gtgggcggcc tgcccgagga catggacttc
     4381 gacctggtcg agccgctgcg agacctgttc aaggacgagg tccggcgggt cggcgaggag
     4441 ctcggcctgc ccgaggagat cgtctggcgc cagccgttcc ccggtccggg cctcggcgtg
     4501 cgcatcattg gcgaggtcac cccggacaag gtcgagctgc tgcaggaggc cgacgccatc
     4561 gtgcgcgccg agctgcacaa ggccggcatg gaccgcgaga tctggcaggc cttcgccgtg
     4621 ctgcccgaca tcaaggcggt cggggtcatg ggcgacgagc gcacctacgg gtacccctgc
     4681 atcatccggg ccgtgacgtc cgacgacgcc atgaccgccg attgggcccg cctgccctac
     4741 gacctgctcg agaagatgtc gagccggatc atcaacgagg tgcccggcat caaccgcgtc
     4801 gcctacgaca tcacgtcgaa gccgcccggc accatcgagt gggaatagac gcccggatcg
     4861 tgtccggttc gtgacgatcc gtggggaaat gcaccatttc ttccacgccc cgcgtcattc
     4921 cgcgccgatc aaggtaggga ttgccccttt cgccgtctaa gtactcctac ggtcggacag
     4981 gaagtggctc cctgctcggc ggaaccacag tcccttcgga ggaacacatg ctcaacgctc
     5041 gatccagatt ccggcgcacc gctcccgcgg ccgcgctgct cggggcgttc gccctgctga
     5101 ccgtcacggc ctccccggcc gcggccgatc ccatcggggc cggcgaggcg tcggccttcg
     5161 gtggtcaggt ccagctgacc gaccaagacg tcctgccgcc caccgcgctc gcggagagca
     5221 ccctggcgga cgaggatgcg gtggcttcgg cgctcgaggt cccggcagac ccgctggcga
     5281 tctccggcac gctgaacgcc agcgcgaagg tgcacgccga ctccgacatc gcgtcggtgc
     5341 tgacccaggt cccccagacg gtcgagggtc cgtacaacgc cgccggtgtc tcctccatcg
     5401 aggacctcac ggtgctcgtc gagcagctcc cggagggtgc ctcgctcgtg tccgccgacg
     5461 tgctgcgcgc cgaagccgca gcggtgtgcc ggggcggcgc cgtggagtac acggcgagct
     5521 cggagatcat cgacctgcag atcggcggcg aggacgtgcc gctgaacgac ccgctgacgc
     5581 aggtcgtcga cggcctgaac gacctgctcg agc
//
LOCUS       DATISU010000111         7064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_657160, whole genome shotgun sequence.
ACCESSION   DATISU010000111 DATISU010000000
VERSION     DATISU010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657160"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1702
                     /locus_tag="VLR27_04640"
     CDS             <1..1702
                     /locus_tag="VLR27_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP02764.1"
                     /translation="TPDLCRHTDRSMPGGRRDPAAERQRAVAARGRLRTGSAEAQGTV
                     SGPPRCVQKRSKRPVSPLSGHQYRHVSMAARVDDPGTPAPSFDSGHFQAFADVGFWEY
                     DLSTEALYLSTQVFQILSIDAPTIEACFGAVHPDDVELLRQVHSRARQQPGPYRVRCR
                     TVDGERVLQIRAQSVAGPDGTPVRYLGMLSDVTADWQLEQALEQSTAARLTGLAATSA
                     VHDLKNLFTVVLGHTHLVTVATAAGHTPEAESLAALERAAQRGLELTSQLLNVGRTGP
                     VTARRVAVAPTLQRLAITAATVLGRHRHLEVDAGDGTVDLLADTTRLERVLVDLVLNA
                     RDALPPQQGSVRVSFRQMPDDEVSQLRESHGIPDGSYGVLEVRDDGTGMDPEVLTHVT
                     DPFFTTKAASGGSGVGMHTVARFVDAAGGALRIESTPGQGTSVQLILPTRPSTAAANR
                     RAGSARVVVSGHDEPRLAALARTLELRGVQAVVSATPSATVRLLRTEPIDLVISDVQP
                     GEDRWLLRTAAATATPVLGVTGTLGGTPPDAPLTDDALEVLAQEVDRLLERAPAHTPA
                     "
     gene            1903..2364
                     /locus_tag="VLR27_04645"
     CDS             1903..2364
                     /locus_tag="VLR27_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP02765.1"
                     /translation="MAQRRNPAPTVLLVDDEADVRLLWRMVFALHGGFGAISEAADGA
                     KAVKMLKTESFEVVVTDFSMPGASGLEVIDATLSGSPSTVVVMVSATADVGQEALRRG
                     ASAFFDKWESTTDLLPRFVTAVLAELEALGDGSHPGWSARPAKHGRALVTR"
     gene            2524..3768
                     /locus_tag="VLR27_04650"
     CDS             2524..3768
                     /locus_tag="VLR27_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSP02766.1"
                     /translation="MTRLLSSFAVLRERTEGRSVDASGRSAPMMTVLWVITTIGLAWV
                     ATYLAGGSSTAVPHAFYVPIIVAAVRFGSPGVLVTSLAAGLVVGPLMPLDVAAGIAQS
                     PENWGTRLVIFVLIGQFTAYLVRHSLPSLTTQLGDRRTRSEIRDAIAQGHLRVEYQPI
                     IDLASGDVVGAEALVRWDHPTRGLLGPDVFVSDAERTGCIDDITRFVLTEACTQVARW
                     RTGVLSGCEQFKIAVNLSGADLSNDNLPVFVGDVLAENGLPNDWLHLEVTETALVADV
                     DAATNSLMALRILGVRLALDDFGTGASSLAHLHHFPLDVVKIDRLFVAQADTHEHSAI
                     LTNGIISLAHTMGLTTVAEGIETLGQAQVLRDFGCEFAQGFLYSRPLRPENFETFYAD
                     RVAFGQQGMPRSARDADRAHWL"
     gene            4240..5973
                     /locus_tag="VLR27_04655"
     CDS             4240..5973
                     /locus_tag="VLR27_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HSP02767.1"
                     /translation="MERIDLDGMLATTTALRAQLGEVRLPLATDDVETARQTADHVRR
                     QLDDYVIPRLRDIDAPVLAVVGGSTGAGKSTLVNSVVGAEVTVPGVLRPTTRSPVLIH
                     HPSAARWFEDDRVLGSLARVRGTPGQPVEGAHQLQLVASAAVPEDLALLDAPDIDSVL
                     DRNREIAQHLLDAADLWVFVTTAARYADAVPWDFLRTASRRGVGIGLVLNRVPPGAGD
                     EIAPHLASMLADEGLGDAPLFVLEEQVLSEGRLPTAAVAPMRAWLDDLAADREARADL
                     VRQSLTGAVADVSTRTEEVASVVEQQQAAADQLAGVATGAFADAHQRLADDVRDGTVM
                     RGEVLARWQDLVGTGELLRELQSRIGRLRDRIAASVTGRPRNVEKFQGAVESGVETLL
                     RERVAEATDEVVLRWRATSAGAALLDAGGDELRRPSDDLGERASRLVRDWQGGLLDLL
                     REEGAGKRTTAKILSYGLNGAALVLMVGVFAQTGGLTGAEVAIAGGSTAVGSTLLEAL
                     LGDQAVRRLTERARADLDRRTGELLADEAERFTSLVDGHRPDTTTARTLRELAAELRR
                     ALGTDAGRRRR"
     gene            5970..>7064
                     /locus_tag="VLR27_04660"
     CDS             5970..>7064
                     /locus_tag="VLR27_04660"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HSP02768.1"
                     /translation="MSRREERRTERADRELRQRLDHLAEVSELAAAGPGRLPTELADD
                     VAATVERAQTRLGRGADRTVVALAGATGSGKSSLFNALVGDDVSPVGVRRPTTSTARA
                     AVFDADGALGDGTADLLDWLQVPQRHVVPTEHAPDLHGLVLLDLPDHDSVEADHRAEV
                     DRLVEVVDAFVWVVDPQKYADAALHEQYLRRFAGHAAVTIVVLNQVDLVPEAGRQGVV
                     DDLARLLEADGLHRVRVVATSTMTGEGVDALRRELAARTAERRALVARLDADVDWLAD
                     QLAGRVSDRAPEPVGRGARRDLASAFADASGADAVADAVGAAHRRRAAQAVGWPPTRW
                     LGRLRPDPLRRLGLDRPPPEPGTTTVGRTSR"
BASE COUNT         1002 a         2615 c         2457 g          990 t
ORIGIN      
        1 aacgccagat ctgtgccgac acaccgaccg gtcgatgcca ggtggcagac gggatccggc
       61 tgcagaacgc cagcgggcgg tggccgcaag agggcgtttg cgcacggggt cggcagaggc
      121 gcagggcacg gtgtccggcc cgccacgctg cgtgcagaaa cggtccaaaa ggcccgtttc
      181 gcccctgagc gggcatcagt accgtcacgt ctcgatggct gcgcgcgttg atgaccccgg
      241 caccccagcg ccgtccttcg acagcggcca cttccaggcc ttcgctgacg tgggcttctg
      301 ggagtacgac ctgagcacag aggcgctgta cctctccacg caggtcttcc agatcctctc
      361 gatcgacgcc ccgacgatcg aggcctgctt cggtgcggtg catcccgatg atgtcgagtt
      421 gctccgccag gtccactctc gggctcgtca gcagccgggt ccgtaccggg tccggtgccg
      481 caccgtggac ggcgaacggg tgctgcagat ccgggcccag tcggtggccg gtcccgacgg
      541 cacgccggtg cggtacctcg ggatgctctc cgatgtcact gctgactggc agctggaaca
      601 ggcactggag cagagcacgg cggcacgcct gacgggcctc gcggccacca gcgccgtcca
      661 cgatctcaag aacctcttca ctgtcgtgct cgggcacacg cacctggtca cggtggccac
      721 cgccgccggg cacacacccg aagcagagtc tctcgccgcg ctcgagcggg ccgcacagcg
      781 ggggctcgag ctcacctccc agctcctgaa tgtcggaaga accggaccgg tcaccgcacg
      841 ccgcgtggcc gtcgccccga cattgcagcg ccttgccatc accgcagcca cggtgctcgg
      901 tcgacaccga cacctcgagg ttgacgccgg cgacggcacg gtcgacctgc tcgccgacac
      961 gacgcggctc gaacgcgtcc tcgtcgacct cgtgttgaac gcccgggacg cgctgccccc
     1021 acagcaaggc tcggtgcgcg tctctttccg acagatgccc gacgacgagg tgagccagct
     1081 gcgcgagtcg cacgggatcc ccgacggaag ctacggcgtg ctcgaagtcc gagacgacgg
     1141 gaccggcatg gaccccgagg tgctcaccca cgtcaccgac ccgttcttca cgaccaaggc
     1201 cgcctcgggc ggaagcgggg tcggcatgca caccgtggcc cgattcgtgg atgccgcggg
     1261 cggggcgctg cgtatcgaga gcacccctgg ccagggcacg tccgtgcagc tgatcctgcc
     1321 gacccggccg tccaccgctg cggccaaccg ccgtgccggc agcgctcgag tggtggtcag
     1381 cggccacgac gagccccgac tcgccgcgct cgcccgcacc ctcgagctca gaggtgtgca
     1441 ggcggtggtc agcgcgaccc cgagcgccac cgtccggctc ctgcgcaccg aaccgatcga
     1501 tctggtcatc tccgacgtcc agcccggcga ggaccgttgg ctgctacgca ccgccgctgc
     1561 gacggccacc cctgtgctcg gcgtcaccgg aaccctggga ggcacgcctc ccgatgctcc
     1621 cctcaccgac gacgctctcg aggtactcgc ccaagaggtc gaccggctcc tggagcgcgc
     1681 tcccgcccac acccccgcct gatcgacaac cggtgcatca acaccgaccc ggtcatcgtg
     1741 gctgcactgg agagcgcgag agagccatcg acctttcagg tttagctgcg gctgacaccg
     1801 gggcaaccgg gacagacgcc cggccgttga ccaaagcgtt gacagttcac caccacactg
     1861 ggagagtggg gcgccacgga gagtgaggga aggggggccg gagtggccca acgaaggaac
     1921 cccgcgccga cggtcctgct cgtcgatgac gaagccgacg tccgcctgct gtggaggatg
     1981 gtgttcgcct tacacggggg cttcggtgcc atctccgagg cggccgacgg tgccaaggcg
     2041 gtaaagatgc tgaagaccga atcgttcgag gtggtcgtga ccgacttctc gatgcctggc
     2101 gcgtcgggcc tcgaggtcat cgacgcgaca ctgtcgggct cgccctcgac cgtggtcgtg
     2161 atggtttccg caacggccga tgttggccag gaggcgctgc gccgcggtgc ttcggcgttc
     2221 ttcgacaagt gggagtccac gacggacctc ctgccgagat tcgtgacagc ggtgctggcc
     2281 gagctcgaag ccctcgggga tggatctcac ccgggttggt cagcgcgacc ggcgaagcat
     2341 ggcagggcgc tcgtgacccg atgagccata cgggcgtgag gtgattgcgg cagtcaagac
     2401 atggttgcgg agcagtggtg cgctggtttc cctcctcgga gcctgaggtt gttgctggcc
     2461 gggaggggta gtgaggtgac gttgacggtc aagttcggca cggttcgcga cgactcatct
     2521 cccatgacgc gccttctgtc gagcttcgcg gtgctgcgtg agcggaccga gggtcgcagc
     2581 gtcgacgcgt ccggtcggag cgcgccgatg atgacggtgt tgtgggtgat caccaccatc
     2641 ggtctggcct gggtcgccac ctacctggcc gggggctcga gcaccgcagt cccccacgcg
     2701 ttctacgttc cgatcatcgt ggccgccgtg cgcttcggct cccccggagt cctcgtgacg
     2761 tccttggccg ccgggctggt cgtggggccg ctcatgccgc tcgacgtcgc cgccggcatc
     2821 gcgcagtcac cggagaactg gggcacccgg ttggtgatct tcgtgctcat cgggcagttc
     2881 accgcctact tggtccgaca ctccctgccg tcgctcacca cgcagctcgg cgaccgccgc
     2941 acccgcagcg agattcgcga cgcgatcgcg caaggtcacc tgcgtgtcga gtaccagccg
     3001 atcatcgacc tcgcttcggg cgacgtggtg ggggccgagg ccctggtgcg ctgggaccac
     3061 cccacccggg gcttgctcgg ccccgacgtg ttcgtctccg acgccgagcg caccggctgc
     3121 atcgacgaca tcacccggtt cgtgctcacc gaggcctgca cgcaggtcgc ccgctggcgc
     3181 accggggtcc tgtcggggtg cgagcagttc aagatcgcgg tgaacctgtc cggcgccgat
     3241 ctcagcaacg acaacctgcc cgtgttcgtc ggcgacgtgc tcgccgagaa cggcctcccc
     3301 aacgactggc tgcaccttga ggtcaccgag accgcgctcg tcgccgacgt cgacgccgcg
     3361 accaacagcc tcatggcgct gcgcatcctc ggcgtgcgcc tcgccctcga cgacttcggc
     3421 accggggcat catcgctcgc acacctgcac cacttccccc tcgacgtggt caagatcgac
     3481 cgactgttcg tagcgcaagc cgatacccac gagcacagcg ccatcctcac caacggcatc
     3541 atctccttgg cccacacgat gggactgacc acggtggccg agggcatcga gaccctgggt
     3601 caagcccagg tactccgcga cttcggctgc gagttcgccc aagggttcct gtactcacgc
     3661 ccactgcgac ccgagaactt cgagaccttc tacgccgacc gcgtcgcctt cggccagcaa
     3721 ggcatgcccc ggtccgcccg cgacgccgac cgcgcccact ggctctgatc aaagccagcg
     3781 catcagacgc gcccgaacgc gaggctgatc actcaccgaa gggctcgggc acgggggaca
     3841 agactcgggc gcgtcccgct gcccgggggc agtcatttcc cgtggagcgg agatctgcgc
     3901 gtcactcgac tgacgcgacg aaggccctcg ccgggggttg ggggggcctt cgtggggccg
     3961 aagtatcttc ggctgaatcc ggtggctggc gggagcacac cgaactcgcc gcgtccatct
     4021 ccacatcccg tctacgtaga accagaacac ccgcagaggt tccggatcgg gactccgaac
     4081 ttgctaccga acgccccttc tccgagggac ccgtggggca gcatcggcca ctcacatggg
     4141 tgcggcacga gttgtcgcta cgacgtggcg cattgccgat gtcggtgccg gccgagtggg
     4201 cggtccaccg ccgctccgac tgggcatgga gcgggcatca tggagcgcat cgacctcgac
     4261 ggcatgctgg cgacgaccac ggccctgcgc gcgcagctgg gtgaggtgcg gctgccgctg
     4321 gccaccgacg acgtcgagac cgctcggcag accgcagacc acgtgcggcg ccagctcgac
     4381 gactacgtca tcccccggct gcgggacatc gacgcgccgg tgctggccgt ggtcggtggg
     4441 tccaccggcg ccggcaagtc gacgctggtc aacagcgtgg tcggtgcaga ggtgaccgtg
     4501 cccggggtgc tgcgccccac cacccggtcg ccggtgctga tccaccaccc ctcggcggcc
     4561 cgctggttcg aggacgaccg ggtgctcggg tcgctcgccc gggtgcgggg cacgcccggg
     4621 caaccggtgg agggcgccca ccagctgcag ctggtcgcct ccgctgccgt gcccgaggac
     4681 ctcgccctgc tcgacgcgcc ggacatcgac tcggtgctcg atcgcaaccg ggagatcgcc
     4741 cagcacctcc tcgacgcggc ggacctgtgg gtgttcgtga ccacggctgc tcgctacgcg
     4801 gacgccgtgc cgtgggactt cctgcgcacc gcgtcccgcc gcggcgtggg catcggcctc
     4861 gtcctgaacc gggtgccgcc cggcgccggc gacgagatcg ccccgcacct ggcctcgatg
     4921 ctcgccgacg agggcctggg cgacgccccc ctgttcgtgc tcgaggagca ggtcctgtcc
     4981 gaaggtcgcc tcccgaccgc ggccgtcgcc ccgatgcgcg cctggctcga cgacctggcc
     5041 gccgaccggg aggcccgggc cgacctcgtc cgccagagcc tcacgggcgc ggtcgctgac
     5101 gtgtccaccc ggaccgagga ggtggcgtcg gtcgtggagc agcagcaggc ggccgccgac
     5161 cagctcgccg gcgtggccac cggcgcgttc gccgacgccc accagcgcct ggctgacgac
     5221 gtgcgggacg gaacggtcat gcggggtgag gtcctggccc ggtggcagga cctggtcggg
     5281 acgggcgagc tcctgcgcga gctccagtcc cgcatcgggc gcctccgcga ccgcatcgca
     5341 gccagcgtca cggggcgtcc ccgcaacgtg gagaagttcc agggtgcggt cgagagcggc
     5401 gtcgagaccc tgctgcgcga gcgggtcgcc gaagccaccg acgaggtcgt gctccgctgg
     5461 cgggcgacga gcgccggcgc cgcgctcctc gacgccggcg gcgacgagct ccgccgcccc
     5521 tccgacgacc tcggcgagcg ggcctcccgc ctcgtccgcg actggcaggg cggcctgctc
     5581 gacctgctcc gggaggaggg cgccggcaag cggaccacgg ccaagatcct ctcctacggc
     5641 ctcaacggcg ccgccctcgt cctcatggtc ggcgtgttcg cccagacggg cgggctcacc
     5701 ggggccgagg tcgccatcgc cggcggcagc acggcggtgg gctccacgct gctcgaggcg
     5761 ctgctcggcg accaggccgt gcgccggctg accgagcgcg cccgggccga cctcgaccgc
     5821 cgcaccggcg agctcctcgc cgacgaggcc gagcggttca cctcgctcgt cgacgggcac
     5881 cggccggaca ccacgacggc caggacgctg cgggagctgg ccgccgagct gcgccgagcg
     5941 ctgggcaccg acgccggacg gcggcggcga tgagccggcg ggaggagcgc cgcaccgagc
     6001 gggccgatcg ggagctgcgc cagcggctcg accacctcgc cgaggtctcc gagctcgcgg
     6061 cggcggggcc cggccggctt ccgaccgagc tggccgacga cgtggccgcc accgtcgagc
     6121 gggcccagac ccggctcggt cggggcgccg accggaccgt ggtggcgctg gccggcgcga
     6181 ccgggagcgg gaagtcgtcg ctgttcaacg cgctcgtcgg cgacgacgtg tcacccgtcg
     6241 gggtgcgccg gccgacgacg tcgacggccc gggccgcggt gttcgacgcc gacggcgccc
     6301 tcggcgacgg gacggccgac ctcctcgact ggctccaggt cccccagcgc cacgtcgtgc
     6361 cgaccgagca cgcccccgac ctgcacggcc tggtcctgct cgacctgccc gatcacgact
     6421 cggtggaggc cgaccaccgg gccgaggtcg accgcctcgt cgaggtcgtc gacgccttcg
     6481 tgtgggtggt cgacccccag aagtacgcgg acgccgcgct gcacgagcag tacctgcgcc
     6541 gcttcgccgg gcacgccgcg gtgacgatcg tggtcctcaa ccaggtcgac ctcgtgcccg
     6601 aggcgggacg acagggcgtc gtcgacgacc tcgcccgact gctggaggcc gacgggctgc
     6661 accgggtccg cgtcgtcgcc acctcgacca tgacgggcga gggcgtggac gccctccgcc
     6721 gggagctcgc ggcccgcacc gcagagcggc gggccctcgt cgcccgcctc gacgccgacg
     6781 tggactggct cgccgaccag ctggccggcc gcgtcagcga tcgagcaccg gagccggtcg
     6841 ggcgtggcgc ccgccgcgac ctggcgagcg ccttcgccga cgcgtcgggt gccgacgccg
     6901 tcgccgatgc ggtcggtgcc gcccaccggc gccgggccgc gcaagccgtg ggctggccac
     6961 cgacccgctg gctgggccgg ctccgccccg acccgctccg ccgcctcggc ctcgaccggc
     7021 cgccgccgga gccgggcacg accacggtgg gtcgcacgtc ccgc
//
LOCUS       DATISU010000112         3751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_145016, whole genome shotgun sequence.
ACCESSION   DATISU010000112 DATISU010000000
VERSION     DATISU010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145016"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /gene="rpsI"
                     /locus_tag="VLR27_04665"
     CDS             complement(<1..155)
                     /gene="rpsI"
                     /locus_tag="VLR27_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HSP02769.1"
                     /translation="MSTKPLVQATGRRKRAVARIRLRPGTGTVTVNGKDVKVYFPTDA
                     LQSLAMAP"
     gene            complement(167..622)
                     /gene="rplM"
                     /locus_tag="VLR27_04670"
     CDS             complement(167..622)
                     /gene="rplM"
                     /locus_tag="VLR27_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HSP02770.1"
                     /translation="MRTYSPKASEITREWWVIDAEGMVLGRLATEVARVLRGKHKAMF
                     APNLDCGDHVIIVNADKVVLTSGKAEKKKVWRHSGFPGGIKSMSYSDALDRKPAEAVR
                     RTIKGMLPKNRLGAQMLTKLKVYAGPTHPHAAQQPKELEIDSRARARVS"
     gene            765..1805
                     /gene="selD"
                     /locus_tag="VLR27_04675"
     CDS             765..1805
                     /gene="selD"
                     /locus_tag="VLR27_04675"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:810..812,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HSP02771.1"
                     /translation="MTQRPRLTAFSHGAGUACKLAPGELAHVVRRLAPPGDPDLLVGA
                     LSGDDAAVWRLSDERALVVTADFITPVVDDARTWGRIAAANAVSDVYAMGGSPLLALN
                     LVGWNVDELPTDLLGDVLQGGLDIATEAGFVVAGGHTVDDPEPKYGMAVIGEVHPDQV
                     LTNAGLRAGDVLVLTKPLGVGIATTAIKRGLASDSLTDAAVGSMTRLNDLARDAARAA
                     GATGCTDITGFGLLGHLQRMAAESGVDVTVEVDAVPVLEGARELLAEGVVPGGSQRNL
                     DHVRPQLDAGPHDDATALLLADAQTSGGLLFGATDASAAAAAVDALVASGHEAAAIGR
                     VVAPGDGRIALH"
     gene            complement(1850..2434)
                     /locus_tag="VLR27_04680"
     CDS             complement(1850..2434)
                     /locus_tag="VLR27_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02772.1"
                     /translation="MPWALEITDAVNERTALNTSLWQGLSGGPLGTLAWSSLVENLTA
                     VEAAFDKLAGDSAYLDLVGKARDWVPVPGEDHILRMVHTAGGDYVRPDVGAYAEGTKA
                     IPAAGKYGKAGEFGVAISDRHSKLTHSSVLFCSVEYGAFGEMRWLGLYESAAEVDHAA
                     ELIAKDDGYRKALDDAEGLFTEGLSQRTLARRIA"
     gene            complement(2669..3448)
                     /gene="truA"
                     /locus_tag="VLR27_04685"
     CDS             complement(2669..3448)
                     /gene="truA"
                     /locus_tag="VLR27_04685"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HSP02773.1"
                     /translation="MTEAPLLPHVRVRMTVAYDGTRFHGFAVNPGVPTVGGRLTEALE
                     RFLRHPIELTCAGRTDKGVHAHGQVVDFVTTEGVDLQGLCRAVNALCGPEVAVREPTI
                     VPMEFSSRFDATSRTYRYTIHNDEVPDPFSVHRAWQVKEPLELDVLRLACDPLIGSHD
                     FTSFCRRPKRTDGTEASLVRDVRSARWEVVDDQTLRFWIQASSFCHQMVRSLVGTLVD
                     VGRGKLTAGDIRGILAAKDRRAAGTVAPPEGLTLWHVGYGD"
     gene            complement(3562..>3751)
                     /gene="rplQ"
                     /locus_tag="VLR27_04690"
     CDS             complement(3562..>3751)
                     /gene="rplQ"
                     /locus_tag="VLR27_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HSP02774.1"
                     /translation="AKKGGLHNHRQVVAYLRDEEMAHKLMTDVGPRYVDRPGGYTRIL
                     KLGPRHGDNAPMARIELV"
BASE COUNT          510 a         1295 c         1385 g          561 t
ORIGIN      
        1 ggggccatgg ccaggctctg gagggcgtcg gtcgggaagt agaccttcac gtccttgccg
       61 ttcacggtga cggtgccggt gccgggacgg agccggatgc gggcgacggc gcgcttgcga
      121 cggccggttg cctggacgag gggcttggtg ctcacgattg cttccttcag ctcacgcggg
      181 cgcgggcccg ggagtcgatc tcgagttcct tgggctgctg ggcggcgtgc gggtgcgtgg
      241 gacctgcgta caccttcagc ttggtcagca tctgggcgcc gaggcggttc ttcggcagca
      301 tgcccttgat ggtgcgacgc acggcctcgg cgggcttgcg gtcgagggca tcgctgtagc
      361 tcatcgactt gatgccgccg gggaagccgg agtggcgcca gaccttcttc ttctcggcct
      421 tgcccgaggt cagcacgacc ttgtcggcgt tgacgatgat cacgtggtca ccgcagtcga
      481 ggttcggggc gaacatcgcc ttgtgcttgc cacgcagcac ccgggcgacc tcggtggcca
      541 gacgaccgag gaccatgccc tcggcgtcga tgacccacca ctcgcgggtg atctcgctgg
      601 ctttcggtga atacgtgcgc acgggtgcga cctgactctc tcggtgtctc gcgactgcgg
      661 atgcgaagct tgctcggggc gcgggaggcc ccgaggcgca agggacaaat ctacgggcgg
      721 gcgctgatcc gctgcaacct ggggtcccgg tagcgtgcca cgcgatgacg caacgacccc
      781 gcctgacggc gttctcccac ggcgccggct gagcgtgcaa gctcgccccg ggcgagctgg
      841 cgcacgtcgt gcgccgattg gcacctcctg gtgatcctga cctcctggtc ggggcgctgt
      901 ccggcgacga tgccgccgtg tggcgcctct cggacgagcg cgccctggtg gtcacggccg
      961 acttcatcac cccggtggtc gacgacgccc ggacctgggg gcgcatcgcg gcggccaacg
     1021 ccgtctccga cgtctacgcc atggggggca gcccgctgct cgccctcaac ctggtgggct
     1081 ggaacgtcga cgagctgccc accgacctgc tgggcgacgt gctccagggc ggcctcgaca
     1141 tcgccaccga ggccgggttc gtcgtcgccg gcggccacac ggtggacgac cccgagccca
     1201 agtacgggat ggccgtcatc ggcgaggtcc acccggacca ggtgctcacc aacgccggac
     1261 tgcgcgccgg cgacgtgctc gtgctcacca agccgctcgg cgtcggcatc gccaccaccg
     1321 caatcaagcg gggcctcgcc tcggactcgc tgacggacgc cgcggtcggc tcgatgacca
     1381 ggctcaacga cctcgcccgc gacgccgccc gggctgccgg cgccaccggc tgcaccgaca
     1441 tcaccggctt cggcctgctc ggccacctgc agcgcatggc ggcggagtcc ggcgtcgacg
     1501 tcaccgtcga ggtggacgcg gtcccggtgc tcgagggcgc gcgcgagctg ctggcagaag
     1561 gggtcgtccc cggcgggagc cagcgcaacc tcgatcacgt ccggccccag ctcgacgccg
     1621 gcccccacga cgacgccacc gcgctgctcc tggccgatgc ccagaccagc ggaggcctcc
     1681 tgttcggcgc caccgacgcg agcgcggccg ccgcggcggt cgacgcgctc gtcgcgtcag
     1741 gtcacgaggc cgctgccatc ggccgggtcg tcgcacccgg cgacgggcgc atcgcgctgc
     1801 actgaccgga ggtcctgccc ggtgacgggc aggaacctcg gccggcggtt caggcgatgc
     1861 ggcgggccag cgtccgctgg gacaggccct cggtgaacag gccctcggcg tcatccagcg
     1921 ccttgcggta gccgtcgtcc ttggcgatca gctcggcggc gtggtcgacc tccgccgcgc
     1981 tctcgtagag cccgagccag cgcatctcgc cgaaggcccc gtactccacg ctgcagaaca
     2041 gcaccgagga gtgggtcagc ttcgagtgcc gatcggagat cgcgacaccg aactcgcccg
     2101 ccttgccgta cttgccggcg gcggggatcg ccttcgtgcc ctcggcgtag gcgccgacat
     2161 ccggtcgcac gtagtcgccg ccggcggtgt gcaccatccg gaggatgtgg tcctcgccgg
     2221 gcacggggac ccagtcgcgc gccttcccga cgaggtcgag gtaggcgctg tcgcccgcca
     2281 gcttgtcgaa ggccgcttcg acggcggtca ggttctcgac cagcgagctc caggccaggg
     2341 tgccgagcgg tccgcccgac aggccctgcc acagcgaggt gttcagcgcg gtgcgctcgt
     2401 tcacggcatc ggtgatctcg agtgcccagg gcatcacctc ctggggcggc gcggagaatg
     2461 tgacgattcg ttggtagatg atcatggtgt gtccccctgg tccgccggtc ggtccgacgg
     2521 ttccgcggtc cagcctccgc ctgggcgggc cggaagtcat cggcgcacag tgcgcccagg
     2581 agacgaaccc gacacggcgc ggagcccacg gcggcaccgg ccgtcggacc ggccacgggc
     2641 agccaccggc gtcgcgcgca gagcggcttc agtccccgta gccgacgtgc cagagggtga
     2701 ggccttcggg gggtgccacc gtgcccgcgg cccggcggtc cttcgcggcg aggatgccgc
     2761 ggatgtcgcc ggcggtgagc ttgccccggc cgacgtcgac gagcgtgccg acgagggagc
     2821 ggaccatctg gtggcagaac gacgacgcct ggatccagaa ccgcagggtc tggtcgtcga
     2881 cgacctccca gcgggcagag cggacgtccc gcaccagcga cgcctcggtg ccgtcggtgc
     2941 gcttcggccg ccggcagaac gacgtgaagt cgtggctccc gatgaggggg tcgcaggcca
     3001 ggcgcagcac gtcgagctcg aggggctcct tgacctgcca ggcccggtgg accgagaacg
     3061 gatcagggac ctcgtcgttg tggatcgtgt agcggtacgt gcgcgaggtg gcgtcgaagc
     3121 gggacgagaa ctccatcggg acgatcgtcg gctcccgcac cgcgacctcg ggtccgcaga
     3181 gcgcgttcac cgcccggcag aggccttgga gatcgacgcc ctcggtggtg acgaagtcca
     3241 cgacctggcc gtgggcgtgc acacccttgt cggtgcgccc ggcgcaggtg agctcgatgg
     3301 ggtggcgcag gaaccgctcc agggcctcgg tgaggcgacc tccgaccgtg gggacgccgg
     3361 ggttcacggc gaagccgtgg aagcgggtgc cgtcgtaggc gacggtcatc cggacgcgga
     3421 cgtggggcag caggggtgcc tcggtcatgt cgtcttcgtc gaagagggtg aggggcgtcg
     3481 ccatggcggg caacaacttc gcacagaacg cgcagcgacc cggcgcaatc gcgccgggtc
     3541 gctccgcagt gctgggtcag ctcagacgag ctcgatgcga gccatcggcg cgttgtcgcc
     3601 gtggcggggg ccgagcttga ggatccgggt gtagccgccc ggacggtcga cgtagcgggg
     3661 gccgacgtcg gtcatgagct tgtgggccat ctcctcgtcg cgcaggtagg cgacgacctg
     3721 gcggtggttg tggagaccgc ccttcttggc c
//
LOCUS       DATISU010000113         1648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_5264, whole genome shotgun sequence.
ACCESSION   DATISU010000113 DATISU010000000
VERSION     DATISU010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5264"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VLR27_04695"
     CDS             complement(<1..366)
                     /locus_tag="VLR27_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HSP02775.1"
                     /translation="MTTTEPRTDRGTELDPDELRRRYELERDKRLRADGNEQYVEMTG
                     QFAHYLEDPYVEPDEREPLFDDVTVALIGGGFAGLITGARLKEAGIDDVRIIEKGGDF
                     GGTWYWNRYPGAQCDVESYI"
     gene            465..764
                     /locus_tag="VLR27_04700"
     CDS             465..764
                     /locus_tag="VLR27_04700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP02776.1"
                     /translation="MSTDAPEQIDLQHDEDGNRFVLRSSGEDVGLIEYRREGDVFDLL
                     HTEVLPQGQGNGLGKVLVRETLDHIRADGGQIVATCPFVARFVDEHDGYADLVAG"
     gene            785..1561
                     /locus_tag="VLR27_04705"
     CDS             785..1561
                     /locus_tag="VLR27_04705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP02777.1"
                     /translation="MTDPSLPEHAAENARYWDDMADEWVAAGERNWAAATPAWGSWAA
                     PEDQVRMLPDDMTGMRAIELGCGTGYVSAWMARRGAEVVGIDVSAEQLATARRLRDEH
                     GLAVELRHESAEAVAEPDGSFDFAVSEYGAAIWCDPYVWIPEAARLLKPGGRLHFLGN
                     HPLAMVCTPLDGSPTEATLHRDWRGMHRFDWRRVEVDPGGVEFNLGIAAWMQLFRDAG
                     FVVEDHLELHDADPSEDRFSVPGWWAHRFPAEQVWKLRRT"
BASE COUNT          226 a          572 c          606 g          244 t
ORIGIN      
        1 gatgtaggac tccacgtcgc actgggcgcc cgggtagcgg ttccagtacc aggtgccgcc
       61 gaagtcgccg cccttctcga tgatgcgcac gtcgtcgatg ccggcctcct tgaggcgggc
      121 gccggtgatc aggccggcga acccgccgcc gatgagggcg acggtgacgt catcgaagag
      181 cggctcgcgc tcgtcgggct cgacgtaggg gtcctccagg tagtgggcga actggcccgt
      241 catctcgacg tactgctcgt tgccgtcggc gcggaggcgc ttgtcgcgct cgagctcgta
      301 gcggcgccgc agctcgtccg gatcgagctc ggtgccgcgg tcggtgcgtg gttcggtggt
      361 ggtcactcat ccccctggat ccccgtgttg gcaacgagcg tacccaaccc cctgaccgcc
      421 gggtcaaggt ggtgggccgc gggccgtcgg gtagggatgg ggccatgtcc accgacgcac
      481 ccgagcagat cgacctccag cacgacgagg acggcaaccg cttcgtcctg cgcagctccg
      541 gcgaggacgt cgggctcatc gagtaccggc gggagggcga cgtgttcgac ctgctccaca
      601 ccgaggtcct cccccagggc cagggcaacg gtctcggcaa ggtgctcgtg cgcgagacgc
      661 tcgaccacat ccgggccgac ggcggccaga tcgtggcgac ctgcccgttc gtggcccgct
      721 tcgtggacga gcacgacgga tacgccgacc tcgtcgccgg ctgagcgggc gtagcgtccc
      781 gccggtgacc gacccgtccc tgcccgagca cgccgccgag aacgcccgct actgggacga
      841 catggccgac gagtgggtgg cggccgggga gcggaactgg gcggcagcca ccccggcctg
      901 ggggagctgg gccgcacccg aggaccaggt gcggatgctc ccggacgaca tgaccggcat
      961 gcgggccatc gagctcggct gcggcaccgg ctacgtgtcg gcgtggatgg cccggcgcgg
     1021 tgccgaggtc gtcggcatcg acgtgtccgc cgagcagctg gcgacggctc gccgcctgcg
     1081 cgacgagcac ggcctggccg tcgagctccg ccacgagagc gccgaggcgg tggccgaacc
     1141 cgacggaagc ttcgacttcg cggtgagcga gtacggcgcc gccatctggt gcgacccgta
     1201 cgtgtggatc cccgaggcgg cccgtctgct gaagccgggc ggtcgactcc acttcctggg
     1261 caaccacccg ctggcgatgg tgtgcacgcc gttggacgga tccccgacgg aggccaccct
     1321 ccaccgggac tggcggggca tgcaccgctt cgactggcgc cgggtggagg tcgacccggg
     1381 tggcgtcgag ttcaacctcg gcatcgccgc gtggatgcag ctgttccggg acgcgggctt
     1441 cgtcgtggag gaccacctcg agctccacga cgccgacccg tccgaagacc gcttctcggt
     1501 gccgggctgg tgggcgcacc gcttcccggc cgagcaggtc tggaagctgc gccgcacctg
     1561 acgtctggcg ccgtgcggga tcgggaacga acgcgccatg cgcatgttcg tgatcctcat
     1621 ggccgtgatc ggcatcgccg tcgtcgtc
//
LOCUS       DATISU010000114         5181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_610936, whole genome shotgun sequence.
ACCESSION   DATISU010000114 DATISU010000000
VERSION     DATISU010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610936"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1681)
                     /locus_tag="VLR27_04710"
     CDS             complement(<1..1681)
                     /locus_tag="VLR27_04710"
                     /inference="COORDINATES: protein motif:HMM:NF020075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HSP02778.1"
                     /translation="MTALLRHRTRPLAVVLAATLLLGLWAAPAAAEEESVRESGGVTT
                     TALPDELTVDGHGYGHGRGMGQYGSLGYATGITGEYWGYQRILAHYYGGTQVGHADDR
                     LMAVWLRSRPSMSAMVVYRADGLAIVGEDDTPPAVRVTPLGENEEGVHEYDVRTGTSC
                     TGPWSTNERIIEGFVRVKKMADSGEKALRLCNSDGSQIAYDDSTELVAIDDKTVNLVR
                     SEEMLRGIVPRESPASWGDLADGAGMNSLRAQSVAARSYALSGDTRWGDLHTRLDADA
                     TTCDDQYCQVYGGVAYVSPTGVITTRTHPNTDRAISETKLEVRRFLNGSLARTEFSSS
                     TGGWSAGGTFPVVEDEGDAVASNPNHSWKTTLTRAQIEAKFPAIGTLNDIQILERNGI
                     GDMGGRVLELRLVGTNGNVDRTGDQFRVDFALKSNWFAISVPPPPPVTARPITTAGPE
                     GQVPRDVFDDVDPDSVHALAVDCVAWREIAEGTSATTFAPHTSVTRGQMASFVARFVI
                     AAGGSLPEDPADAFDDDEDSVHEDAINQLAAVGIVRGTGVRTYEPSTRIDRG"
     gene            complement(1688..2944)
                     /locus_tag="VLR27_04715"
     CDS             complement(1688..2944)
                     /locus_tag="VLR27_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HSP02779.1"
                     /translation="MVAATLLVALLPVLTPAAAGAETAGAPDAYDSVIDITFPTVPEK
                     VRFGDDFDAPRSRGAHGATDLMGPKLTPIFAARGGTVVRMGDGGHTLSGYYLTIAGDD
                     GRSYSYIHLNNDTPGTDDGAAPPEQAYAPGIGLGAEVERGQHIGWMGDSGNAENAGSH
                     LHFSISDPAITDPDGTDRRNPYPSLVDALDRGDVSTATVHPLGSAPPPPPAPATGPIP
                     DSSALCGGTVTTQSFTDVTTANVHLGPIECLAARDVALGFTDGRYGPGDTVTRLQMAS
                     FVARLLEAGGVTLPEPSTDHFDDDAGSPHERAVNQLVELRIIRMDTGEVGRTFYGSID
                     MKRDRMAAWMTRAYELVAGQALASTSTDFFRDDDRFHEGDINRLAAAGIVQGTSPGTY
                     EPRTSVRRDQMASYLVRTLAAAAAVR"
     gene            3181..4728
                     /locus_tag="VLR27_04720"
     CDS             3181..4728
                     /locus_tag="VLR27_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HSP02780.1"
                     /translation="MLVRRTVLAGALAMSMVAGPLAAGPAHAADDPPPVADPIGDGNV
                     CEGAPEESPFEDLSAEQDGTAAAIRCLVTAGITKGTTDDTYGPGASVTRRQMALFVKR
                     LADLLDDLDVEDGVEALPAYDGVVDYPDVALESAEVQEAIGQLDQAEIVGGHDDGMFR
                     PGDPVTRRQMAAFVNRLQAHLVGEAFADDGDYFTDDDGDPGEANLDALAAVGIFQGEG
                     DGRVVPGGQITRRQMANVLLRHAQVLFADDLIVAPFVAPTAPAKPTAKAQDLTVTVTY
                     VRPAANPDSTSYALELAEVAQGPDGFCGNDDDVAPADADFTRIEATETEGDEDGTWTL
                     TADDVEKGCYRFRVAAVALGGAATESPETTPSTWVTDAGDDTAPTSVNAYVTEGDGED
                     GVLDAPDVITIIFSEFVRVADDASMTIVDGDGTSVELADDGTTVSFDLNTTAVVIDGT
                     EHRPGHVLSSVVLDDLETSFPGLSAGLQLPADIASADGITDAAGNAWAPSLLGDGGGD
                     TELEAAE"
     gene            complement(4731..>5181)
                     /locus_tag="VLR27_04725"
     CDS             complement(4731..>5181)
                     /locus_tag="VLR27_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02781.1"
                     /translation="LALVSSPWVINPVVRWAAARRGLREVPEVGWSGYAELVGHLVLF
                     WLASAAAFWSYLAAFPAVDAPGVVRTAGTFLLSWAAGFVAIFAPQGVGVFEATMAGLL
                     DGPVAALALVIAGYRALTAVRDALALVALALARGTLPRAVEPPSSSS"
BASE COUNT          714 a         1773 c         1970 g          724 t
ORIGIN      
        1 gcccgcgatc gatgcgtgtg ctcggctcgt acgtgcgcac gccggtgccg cgcacgatgc
       61 cgactgccgc cagctggttg atggcgtcct cgtggaccga gtcctcatcg tcgtcgaacg
      121 cgtccgccgg gtcctccggc agcgacccgc cggcggcgat gacgaagcgg gcgacgaagg
      181 aggccatctg cccccgggtc accgaggtgt ggggggcgaa ggtggtggcg ctggtgccct
      241 cggcgatctc gcgccaggcg acgcagtcga cggcgagggc gtggacgctg tccgggtcga
      301 cgtcgtcgaa gacgtcgcgg gggacctgcc cctccgggcc tgccgtggtg atgggccggg
      361 cggtcaccgg cgggggtggc ggcacggaga tcgcgaacca gttggacttg agggcgaagt
      421 cgacgcggaa ctggtcgccg gtgcggtcga cgttgccgtt cgtgccgacc agccgcagct
      481 cgaggacccg gcctcccatg tcgccgatgc cgttgcgctc caggatctgg atgtcgttca
      541 gcgtgccgat cgcggggaac ttcgcctcga tctgggcccg ggtcagcgtg gtcttccagc
      601 tgtggttcgg gttgctcgcc acggcgtctc cctcgtcctc gacgaccggg aaggtgccgc
      661 cggcggacca gccgccggtg gaggaggaga actcggtgcg ggccaggctg ccgttcagga
      721 agcgacgcac ctcgagcttc gtctcgctga tcgcccggtc ggtgttgggg tgcgtccggg
      781 tggtgatgac gccggtgggg ctgacgtacg cgaccccgcc gtagacctgg cagtactggt
      841 cgtcgcaggt ggtggcatcg gcgtcgaggc gggtgtggag gtcaccccag cgggtgtcgc
      901 cggacagcgc gtagctgcgg gcggcaaccg actgcgcccg cagggagttc atgccagcgc
      961 cgtcggccag gtcaccccag gaggccggtg actcccgggg gacgatgccg cgcagcatct
     1021 cctcggagcg cacgaggttg accgtcttgt cgtcgatggc gaccagctcg gtgctgtcgt
     1081 cgtaggcgat ctggctgccg tcgctgttgc acagccgcag cgccttctcg ccgctgtcgg
     1141 ccatcttctt cacccggacg aagccctcga tgatgcgttc gttggtgctc cacggcccgg
     1201 tgcaggaggt gccggtccgc acgtcgtact cgtgcacgcc ctcctcgttc tcgcccagcg
     1261 gcgtgacgcg cacggccggg ggcgtgtcgt cttcgccgac gatcgccagg ccgtcggccc
     1321 ggtacacgac catggcgctc atggacggcc ggctgcgcag ccacaccgcc atcaggcggt
     1381 cgtcggcgtg gccgacctgg gtgccgccgt agtagtgggc caggatgcgc tggtagcccc
     1441 agtactcacc ggtgatgccg gtggcgtagc cgagtgagcc gtactggccc atgcctcggc
     1501 cgtggccgta gccgtgcccg tcgacggtga gctcgtcggg gagggccgtg gtggtcacgc
     1561 cgcccgactc gcgcaccgac tcctcctcgg cagccgccgg ggcggcccac aggccgagga
     1621 gcagggtcgc ggcgagcacg accgcgagag gacgcgtgcg atgacggagc agagcggtca
     1681 cgctgggtca gcgtacggcg gcggcagctg ccagggtgcg caccaggtac gacgccatct
     1741 ggtcgcggcg gacgctggtg cgtggctcgt acgtgccggg gctggtgccc tgcacgatgc
     1801 cggcggcggc gagccggttg atgtcgccct cgtggaagcg gtcgtcgtcc cggaagaagt
     1861 cggtgctcgt ggacgcgagc gcctggccgg cgacgagctc gtacgcccgg gtcatccagg
     1921 ccgccatgcg gtcgcgcttc atgtcgatgc tgccgtagaa ggtgcgaccg acctcaccgg
     1981 tgtccatgcg gatgatgcgc agctcgacga gctggttcac ggcccgctcg tgcggggagc
     2041 cggcgtcgtc gtcgaagtgg tcggtgctcg gctcgggcag cgtcacgccg ccggcctcga
     2101 gcaggcgggc cacgaaggag gccatctgca gccgggtgac cgtgtcgccc gggccgtacc
     2161 ggccgtcggt gaagccgagg gccacgtcgc gcgctgccag gcactcgatc ggcccgaggt
     2221 ggacgttggc ggtggtgacg tcggtgaacg actgggtggt cacggtgccg ccgcacagcg
     2281 cgctcgagtc ggggatcggg ccggtggcgg gagccggcgg cggcgggggg gcggacccca
     2341 gcgggtggac cgtcgcggtg gacacgtcgc cccggtcgag cgcgtcgacc agcgacgggt
     2401 acgggttgcg ccggtcggtg ccgtcggggt cggtgatcgc cgggtcgctg atgctgaagt
     2461 gcaggtggga gccggcgttc tcggcattgc ccgagtcgcc catccagccg atgtgctggc
     2521 cccgctcgac ctcggcgccg agaccgatgc cgggtgcgta ggcctgctcg ggcggcgcgg
     2581 cgccgtcgtc ggttccgggg gtgtcgttgt tgaggtggat gtagctgtag ctgcggccgt
     2641 cgtcgcccgc gatcgtgagg tagtagcccg agagggtgtg gcccccgtcg cccatgcgga
     2701 ccaccgtgcc gccacgggcg gcgaagatcg gcgtgagctt gggtcccatg aggtctgtgg
     2761 ccccgtgcgc cccgcgggaa cggggcgcgt cgaagtcgtc tccgaaccgc accttctccg
     2821 gcacggtggg gaacgtgatg tcgatcacgc tgtcgtaggc gtccggggcg cctgccgtct
     2881 cggcgcccgc cgcagccggc gtgagcaccg gcaggagggc gacgagcagc gtggcggcga
     2941 ccatcgcagc gaggcggcgg ggaatgtgtc gatctgatgt cgtcgagatg tcgaccatgg
     3001 tcgcattgat cggcacggca cgcgaagttc tgaagcccgt ccggcccgat tgtgccgaac
     3061 gtcccatgtg tcgctcatga cgacggttgt ggccgagggg gacatcgcgg gcccggcgtc
     3121 gaaccatccc ggcggcggtg tcgaagacgg caccgcagac ttcgagcaag tggagggaac
     3181 atgttggtgc gacgcacggt gctcgctggc gccttggcga tgtcgatggt ggcggggccg
     3241 ctcgcggcag ggccggctca cgcggcggat gatccgcccc ccgtcgccga tccgatcggc
     3301 gacggcaacg tgtgcgaggg cgccccggag gagagcccct tcgaggacct ctccgcagag
     3361 caggacggca ccgctgcggc catccggtgc ctggtgaccg ccggcatcac caagggcacc
     3421 accgacgaca cctacggccc gggtgccagc gtgacccgcc gccagatggc gctgttcgtg
     3481 aagcgcctgg ccgacctcct cgacgacctc gacgtggagg acggcgtgga ggcgctgccg
     3541 gcgtacgacg gcgtcgtgga ctaccccgac gtggccctgg agagcgccga ggtccaggag
     3601 gccatcggcc agctcgacca ggccgagatc gtcggcgggc acgacgacgg catgttccgc
     3661 cccggcgacc cggtcacccg ccgccagatg gctgcgttcg tgaaccggct gcaggcccac
     3721 ctggtgggcg aggccttcgc cgacgacggc gactacttca ccgacgacga cggcgacccc
     3781 ggcgaggcca acctcgacgc gctcgccgcg gtcggcatct tccagggtga gggcgacggc
     3841 cgggtggtgc ccggtggcca gatcacccgc cgccagatgg ccaacgtgct gctccgccac
     3901 gcccaggtcc tcttcgcgga tgacctgatc gtggcgccgt tcgtcgcgcc gaccgcgccg
     3961 gcgaagccca ccgcgaaggc ccaggacctc acggtcaccg tcacctacgt gcgcccggcc
     4021 gcgaaccccg actccaccag ctacgccctc gagctggcgg aggtggcgca gggtccggac
     4081 gggttctgcg gcaacgacga cgacgtcgcc cctgcggatg ccgacttcac ccggatcgag
     4141 gccaccgaga ccgagggcga cgaggacggc acgtggaccc tcaccgccga cgacgtggag
     4201 aagggctgct accgcttccg ggtggccgcc gtcgcgctgg gaggcgccgc cacggagtca
     4261 ccggagacga cccccagcac ctgggtgacc gatgcgggcg acgacaccgc gccgacctcg
     4321 gtgaacgcct acgtcaccga aggtgacggc gaggacgggg tgctcgacgc ccccgacgtg
     4381 atcaccatca tcttctcgga gttcgtgcgg gtcgccgacg acgcgtcgat gacgatcgtc
     4441 gatggcgacg gcacctcggt cgagctcgcc gacgacggga cgacggtgtc gttcgatctg
     4501 aacaccacgg ccgtcgtgat cgatggcacc gagcaccggc ccggccacgt gctgagcagc
     4561 gtggtcctcg acgacctgga gaccagcttc cccgggctca gcgccggcct gcagctgccg
     4621 gccgacatcg ccagtgcgga cggcatcacc gacgccgccg gcaacgcgtg ggccccgtcg
     4681 ctgctcggcg acggcggtgg cgacaccgag ctggaggctg ccgagtagtc ctagctcgac
     4741 gagctcggcg gctcgaccgc ccgcggcagg gtgccgcggg cgagggccag ggcgaccagc
     4801 gccagcgcgt cgcgcacggc ggtgagcgcc cggtagccgg cgatcaccag ggcgagggcg
     4861 gcgacggggc cgtcgagcag accggccatc gtggcctcga acaccccgac gccctggggg
     4921 gcgaagatgg ccacgaaccc ggcggcccag gacagcagga aggtgccggc ggtgcgcacg
     4981 acccccgggg cgtcgacggc ggggaaggcg gccaggtagg accagaacgc cgccgccgac
     5041 gccagccaga acaggaccag gtgcccgacc agctcggcgt agcccgacca gcccacctcg
     5101 ggcacctcgc gcagcccacg gcgggccgcc gcccagcgca cgaccgggtt gatcacccac
     5161 ggtgagctga ccagggccag c
//
LOCUS       DATISU010000115         3905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_517783, whole genome shotgun sequence.
ACCESSION   DATISU010000115 DATISU010000000
VERSION     DATISU010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517783"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VLR27_04730"
     CDS             <1..239
                     /locus_tag="VLR27_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02782.1"
                     /translation="PVVAAGVEPDVPAAVFVANRVVACTAAARAAGVRRHMRRREASR
                     PRRAELWLPSVDGTVVEVLPEPVPLRAPRGSEIA"
     gene            302..709
                     /locus_tag="VLR27_04735"
                     /pseudo
     CDS             302..709
                     /locus_tag="VLR27_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1685..2731)
                     /locus_tag="VLR27_04740"
     CDS             complement(1685..2731)
                     /locus_tag="VLR27_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSP02783.1"
                     /translation="MRSRHASLLTAVAPALWGTTYIVTTELLPPGRPLLAAALRALPA
                     GLAVIAITRTLPRGDWWWRAAVLGTCNFGIFFSLLFTAAYRLPGGVAATVGAVQPLLV
                     MLLAAGLLRERATSTKVLAGLAGVVGVALLVLDGSAQLDPVGVAAALGGAASMATGSV
                     LVQRWGRPTGALTFAGWQLTAGGVVLLPMALVVEGTPEALTATNLAGYGYLAVLGGAL
                     AYTLWFRGIERLGAGNVTFLALLSPLVATTVGVVAGERLSPTQALGAATVLGSVVTVQ
                     RAARRGLVPVTSVVGGGDRPPATLEDDEVRGAVVGDDLVADPAGVPLRVPPALVDRPR
                     TSPRLDAPAPAAGS"
     gene            2784..3311
                     /locus_tag="VLR27_04745"
     CDS             2784..3311
                     /locus_tag="VLR27_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSP02784.1"
                     /translation="MSQDAFQQDESDAVDRIVEQWRTERPDLDPSAKGITGRIVRLAG
                     SFQRAYGEAFAPLELSEGDYGVLVALRRAGDPYQLTPTALARTRMMTSGGMTAGLDRL
                     ERRGLLERRPNPDDRRGSIVALTPEGLAKIDEAMAIHARVEHELVASLPAAQRDQLAA
                     LLRSLVLGTEGPGDA"
     gene            3362..>3905
                     /locus_tag="VLR27_04750"
     CDS             3362..>3905
                     /locus_tag="VLR27_04750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP02785.1"
                     /translation="MSSRRRTSTAEGRELIGLLAMATALTALGIDLMLPAFDEIRADF
                     DLGAGSTAVTGLVTAYFVGLGLGTVFYGPLADHKGRRWTMRLGLGIYIAGALLAAVAP
                     TLPMVMLARFLWGMGAAGPRVIALAIVRDRFEGDAMSRTMSSLMAVFLLVPVLAPTLG
                     ALLLEVAPWRWLFVLCAVGAL"
BASE COUNT          452 a         1510 c         1459 g          484 t
ORIGIN      
        1 ggccggtggt ggcggccggg gtggagccgg acgtgccggc cgcggtgttc gtggccaacc
       61 gggtggtggc gtgcacggcc gcggcgcgcg ccgccggggt gcgtcgccac atgcgccggc
      121 gcgaggcgtc ccgtccccgc cgggccgagc tgtggctgcc gtcggtcgac ggcacggtgg
      181 tggaggtcct gcccgagccc gtgcccctgc gggcgccccg ggggagcgag atcgcatgac
      241 gcagctcgcc cctcgctagg tccagccggt cgggccgccg gtcgacgagc cggtgcgcac
      301 catgtcggtg tgggtgcccg actggccggt ggtggcggcc ggggtggagc cggacgtgcc
      361 ggccgcggtg ttcgtggcca accgggtggt ggcgtgcacg gccgcggcgc gcgccgccgg
      421 ggtgcgtcgc cacatgcgcc ggcgcgaggc ccagtcccgc gcgccgtccc tcctggtgct
      481 cgaccacgac cccgaccgcg acgcccgagc attcgaggcc gtggtgcagg ccatcgaggc
      541 gctcacgccg cgcatcgagg tcacccgacc gggccggtgc gcgttcccca cccgtgggcc
      601 gtcccgctac ttcggcggcg acgaggccct ggcggcccgg gcgctcgacg tcgtggccga
      661 ggtcctcgcc gtcccggtgg cggtgggcgt cgccgatggt cccttcgccg ccgcccacgc
      721 cgcccgcacc gcgctcgtgc gggcgaaccg atcgggtggg gctgagccgc cggtgtgggt
      781 cgtggccccg ggccgggccg ccgagtacct ggcgccgctg tccctcgggg ccctcgaccg
      841 gcccgagctg gccgacgtgc tcgcccgcct cggcctgcac tccctcggcg atctcgccgc
      901 cctgcccgcc gccgacgtgc tcggccgctt cgggtccgaa ggactcgaag cccaccgcct
      961 cgcatgcggc ctcgaccccc gcccgccggc caccgaaccg ccaccccccg acctggtcgt
     1021 gtccgccgag ctcgacccgc cggccgagca ggtcgccccc atcgcgttcg tcgcccgggg
     1081 catggccgac gagctccacg cccgcctcga ggcccgggga tcggtgtgca cccgcatcgt
     1141 gatcagcgcc gagaccgagc acggcgagcg gtccgagcgg gtgtggcgcc acgagggggc
     1201 gctcagcgcc ggcgccatcg ccgaccgggt ccgctggcag atggacggct ggctccacgc
     1261 caacgtccgc cagcgcccct ccgccggcat cgcccacctc tggctccacc ccgacgaggt
     1321 catcgccgcc gacggccgcc agctcggctt ctggggggcc gacaccggcg cggccgatcg
     1381 agccgtgcgg gccatcgccc gggtccaggg cctgctcggc accgatgccg tcctcgtgcc
     1441 cgagtacgcc ggcggccggg gcccgggcga gcagctccgg ctcgtgccgg ctgcggccgt
     1501 cgacctcggc gccgaccgtc ccgcggcccg gcccgactgg gtgacggccc cgtggccggg
     1561 ccggctgccc aacccgtcac cggccaccct gcaccccgat gccgtgccgg tcgacgtgct
     1621 cgacgcccgg ggtcgccggg tggtggtcga cgggcgctgc cggcccacgg ccgagccagc
     1681 agcgctacga gccggccgcc ggcgccggcg catcgagtcg tgggctggtc cgtggccggt
     1741 cgacgagcgc tggtgggacc cggagcggca ctcccgccgg gtccgctacc aggtcgtcac
     1801 cgacgacggc gccgcgcacc tcgtcgtcct cgagggtggc cggtggacga tcgccgccac
     1861 ctacgactga ggtcacgggc acgagcccac ggcgggcggc ccgctgcacc gtgaccacgc
     1921 tgcccagcac ggtggcggcg ccgagtgcct gggtggggga gaggcgctca ccggcgacca
     1981 cgccgaccgt ggtggccacc agcgggctca gcagtgccag gaacgtgacg ttgccggcgc
     2041 cgagccgctc gatgccgcgg aaccacaggg tgtaggcgag cgcgccgccc agcacggcga
     2101 ggtagccgta gcccgccagg ttcgtcgccg tcagcgcctc gggcgtgccc tcgacgacga
     2161 gcgccatcgg gagcagcacg accccgccgg cggtgagctg ccagccggcg aaggtcaggg
     2221 caccggtcgg ccggccccag cgctgcacca gcaccgagcc ggtggccatc gaggccgcac
     2281 cgccgagcgc ggcggcgacg cccacggggt cgagctgcgc gctcccgtcc agcacgagca
     2341 gcgccacccc gaccacgccg gcgagcccgg cgagcacctt ggtggacgtc gcccgctctc
     2401 ggagcagccc ggcggcgagg agcatgacga gcaggggctg cacggcgccg acggtggcgg
     2461 cgacgccgcc cggcaaccgg tacgccgcgg tgaacagcag cgagaagaag atcccgaagt
     2521 tgcaggtgcc gagcacggcc gcccgccacc accagtcgcc gcgggggagc gtccgcgtga
     2581 tcgcgatcac ggcgaggccg gccgggaggg cgcggagagc ggcggccagc agcggtcgcc
     2641 cgggagggag cagctcggtg gtgacgatgt aggtggtgcc ccacagcgcc ggcgccacgg
     2701 cggtgagcag gctggcgtgg cgggatcgca tcggaactcc tcgagatcga gtatcttgct
     2761 gcaaaggtag attggagatg acaatgagtc aagatgcttt ccagcaagat gaatcggacg
     2821 cggtcgatcg catcgtcgag cagtggcgga ccgagcgtcc ggacctggac ccctcggcca
     2881 aggggatcac cggtcgcatc gtgcgcctgg ccggcagctt ccagcgggcc tacggcgagg
     2941 cgttcgcccc cctggagctc agcgagggcg actacggcgt cctcgtcgcg ctccgccggg
     3001 ccggggatcc gtaccagctc acgccgacgg cgctggcccg gacgcggatg atgacctccg
     3061 gcgggatgac cgcggggctg gaccgcctgg agcggcgtgg cctgctcgag cggcggccca
     3121 accccgacga ccggcgcggg agcatcgtcg ccctcacccc cgagggcctt gccaagatcg
     3181 acgaggcgat ggccatccac gctcgggtcg agcacgagct ggtggcgtcg ctgcccgcgg
     3241 cccagcgcga ccagctcgcc gcgctgctcc ggtcgctggt cctcggcacc gaggggcccg
     3301 gcgacgcgtg aacgcccgat ccgaggtggg ggcgctcgga tcgacccggc agactcctcc
     3361 ggtgagcagc cgccgtcgca cgtccaccgc cgagggtcgc gagctcatcg gcctgctcgc
     3421 catggccacc gcgctcaccg cgctcggcat cgacctgatg ctgccggcct tcgacgagat
     3481 ccgggccgac ttcgacctcg gcgccggctc caccgcggtc accggtctcg tcactgccta
     3541 cttcgtgggc ctcggcctcg gcacggtgtt ctacgggccc ctcgccgacc acaagggccg
     3601 tcgctggacc atgcgcctcg gcctcggcat ctacatcgcc ggcgccctgc tggctgcggt
     3661 ggcgccgacg ctgccgatgg tcatgctcgc ccgcttcctc tggggcatgg gcgctgccgg
     3721 tccccgcgtc atcgccctcg ccatcgtgcg cgaccgcttc gagggcgacg ccatgtcgcg
     3781 gacgatgtcg tcgctgatgg ccgtgttcct cctcgtcccc gtgctcgccc cgaccctcgg
     3841 cgcgctgctg ctcgaggtcg cgccgtggcg ctggctgttc gtgctctgcg ccgtcggcgc
     3901 cctgc
//
LOCUS       DATISU010000116        11663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_657399, whole genome shotgun sequence.
ACCESSION   DATISU010000116 DATISU010000000
VERSION     DATISU010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 11663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11663
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657399"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2266)
                     /locus_tag="VLR27_04755"
     CDS             complement(<1..2266)
                     /locus_tag="VLR27_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HSP02786.1"
                     /translation="MFARLGTWCHDHRRTVLGLWIATLVLGGVISGAAGSAFRDEFSL
                     PDVESKDGFDILDEEFGGQGTGMTGTVVFRADQGVDDATVQAVMTDLFAAIAQIDDVD
                     RVDSPYAPEGGRQISSDGTIAYANVELPEDVDFERAVEIRDEILEAAPTVDGLQLELG
                     GYIFAEFEEPTAELLGLAFAIVVLILAFGSVLAMGLPVGVALFGIGIGTTIITLLSHV
                     LSIPDFATFLGIMIGLGVGIDYALLIVTRFREQMHQGHTVADSVAVALDTAGRAVLFA
                     GTTVVISLLGMLLMQVSFVQGLAVGAASVVAVTVVASLTLLPALLGFAGDRVERTRWR
                     GLVAAGFVALALAGMGLKVTPLVIGLPVAAVVLIAGTWIPFLRREVPARAQKPRRETA
                     AYRWSRVIQHRPWPAAIAGAAILLLLAGPTLSLRLGFSDESNFEESTTTRQAYDLMVE
                     GFGPGFNGPFMIVARTDDPGALGSITDAVSADPGVAFVSPAVPNDPSAPTAVLWNVVP
                     TTGPQDVATERLVHRLRDDVLPPIEDRAGVDLAVTGTVPVNIDFAQFLTERMPYFFGA
                     VLALSFLLLMVVFRSLLVPLKAVIMNLLSIGAAYGVVVALFQWGWLSDLTGVQPAPIE
                     PWAPMMLFAIVFGLSMDYEVFLLSRVREEWLRTGDSRESVADGLAATAKVITAAAAIM
                     VVVFGAFILEPDRVMKLMGTGLATAIFLDATIVRMLLVPATMELLGDRNWWLPGWLDR
                     LLPTIDVEGHTEPVA"
     gene            2576..4369
                     /locus_tag="VLR27_04760"
     CDS             2576..4369
                     /locus_tag="VLR27_04760"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HSP02787.1"
                     /translation="MRRALTAAVVAALLVAGPLAASPAGAVGGTTSTTVVHGIPDLTV
                     DVYVDGAKAIDDFVPGTVAGPLTLPAGTRSVAITAADAMDDSDPVLAGDIVLVAGADM
                     SIVAHLDGAGDPTVSAFTNDTSAIAEGEGRITVRHLAAAPAVDILAGGAVAFAGLTNG
                     ASAAADLPAGTLPVSINAAGTSTQVFPAEGTLDVALPAGVNLVVYAWGDFAAGSFALA
                     TQTIGEGTIGAPSQVSVVHGVPGLTVDVYLNGVLLLEDFEPTQVAGPVLLPAGTYDIE
                     VFAADAAYAPGGGAITVDDAVVPAGANISAVAHLDEEGAPTASLFVNDTSPIDDGEAR
                     LVVRHTAAAPTVDVLTGDTKLIDSITNPESQQLDVPAGTYPAAVHAEDDPEPAVDLGD
                     VTLPAGQATIVYAIGELGSTFTVAAQSIVGLAAGGAFADTGADQPSSDDVRTLLQLGI
                     TNGTSPTMYSPSECVTRGQMAAFLVRTLELPASDVDAFGDDDGTFFEDEINALAAAGI
                     TAGTSATTFSPDQCVTRGQMAMFLARGFNVPASNVDAFGDDDGTTAEPAINAIAAAGV
                     TMGTTPTTFSPGEHVSRGQMASFLARSLGVG"
     gene            complement(4397..4993)
                     /locus_tag="VLR27_04765"
     CDS             complement(4397..4993)
                     /locus_tag="VLR27_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP02788.1"
                     /translation="MTRGSARQRLLDAAIDHVAEHGLADLSLRSLATELGTSHRMLSH
                     HFGSKDGLWLAIVREVERRQLEAFTELDPDPSLPLDDLMRTWWQHISAPALWPNERLF
                     FEVYAQALHDRPPANELLDGVVEPWVRPAAALAEAAGVPPETAEAFARLGLAVTRGLL
                     LDLLATQDRAAVDAAMEHWITLSTGYMETARRGATVER"
     gene            5041..5499
                     /locus_tag="VLR27_04770"
     CDS             5041..5499
                     /locus_tag="VLR27_04770"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSP02789.1"
                     /translation="MLKPVTVSIDVDHDIEDVFAFLDVMANHEPFNDHLMTDWELSGP
                     ERGVGSKARVRTRALGMTDAVDIEVIEAQAPTTIVERNVAAKAGRTGEGTYTLSRLDG
                     GGTRITFEYRWIVTPVLDRITKPIARAYIRRNNTTSMRRLARQLDLELAA"
     gene            complement(5544..8573)
                     /locus_tag="VLR27_04775"
     CDS             complement(5544..8573)
                     /locus_tag="VLR27_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HSP02790.1"
                     /translation="MTKGGGAMQIRVLGPLEVISGDGTPCAVRGARRRLLLATLVLHR
                     NATCGIDQLVDVLFGQDPPERAAGTVQSYVSRLRGDLGDDGSRLQTRPGGYQLTIDPD
                     ELDSVRFERQVASALGVFGREPQRAADLLKEALGWWSGGRAFGEFVDDLGLQAESTRL
                     EEVRQRAAEARVDALLALADHAGAIDELETCIASWPLREGFRAQQMLALYRSGRQPEA
                     LRAFHRFRSDLGDQLGLEPSEALADLEARMVRTDPTLDAPPVAPPSGPPPPAPADGEP
                     RGNLPRRVTALLGRDVELSQLTEVLGTVPLLTLTGPGGVGKTRLAMQLGERAATEHPD
                     GVWLCDLAPVREDALVAEAVTTALDVQRRQDRTTIEGLVEVLQSRRLLIVLDNCEHVL
                     APIREVADAVLRACAGVQIVATSREPLGVEGETVWPLGPLPLPRADETDPVMAMESPA
                     VRLFVARATSAHPGFRLDEATVPSVVEICRRLDGLPLALELAAARVRSMTLPDLADEL
                     AGDFRILVTPAGDPRHQTLEATVAWSYQLLGVAEQALFDRLSVFAGSFTVDDIEYVCA
                     DATVSAEGIRGLLAALVDKSMVVADISRSPTRYSLLETLRQFGGAQLRLAGCADVLQE
                     RHALWAIDLVEHAEVGLGGPDEPVWMSRLDDTFADLRIAHRWMLTQGDEDGALRLVVG
                     PREFAFRRMRYELFSWAEATLELLTTDDHPLLPMALATAAYGRFVRGDLDAATALAER
                     SLEVEERLGLPSCGLHSRTLGNVYYYRGQTFEAAETCQRTVGAARASGDDARLAHALY
                     MASVGLASDARTEASRLLADEAVSLARRIGNPTSLASALYARALTLEAIDPDRATSML
                     EESIAHGTAAGNRWIVAFAQTELVSLAGRRGDLDGALRLAREVIDMWHRAGDWANQWL
                     TLRHVAGVLAQRGDHEAAAMLHAAVRLASAELAMPIEASDLRRVGAILEQLPSALGHT
                     RYAEAEARGAAMGADTVVQSTRAVIDRVLAGVGPDAA"
     gene            8687..8911
                     /locus_tag="VLR27_04780"
     CDS             8687..8911
                     /locus_tag="VLR27_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HSP02791.1"
                     /translation="MPFVNVKLIEGVFSADQKRDMIAKLTDTMVAIEGENMRGVTWVV
                     LEEVTSGEWGIGGQALSTADVKALAAGAPA"
     gene            complement(8994..9458)
                     /gene="arr"
                     /locus_tag="VLR27_04785"
     CDS             complement(8994..9458)
                     /gene="arr"
                     /locus_tag="VLR27_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HSP02792.1"
                     /translation="MAASHDTEHVPVTFECCDHVDGPFFHGTRTTFEVGDEIVPGHPS
                     NYQDGRTSNHVYFAALLEPAVWAAELANALAGSDDRGRIYVVEPTGPFEDDPNVTNKR
                     FPGNVTRSYRTRHPMLVVSEVESWDGHPPEVLQGMLDNLTRLREQGLDVIED"
     gene            9559..10152
                     /locus_tag="VLR27_04790"
     CDS             9559..10152
                     /locus_tag="VLR27_04790"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP02793.1"
                     /translation="MRDVTPSDTDRPMRVDARRNRRRLLDAAIGLILEIGGEPSRDAV
                     AQRAGVGIGTLYRHFPDRQSLLHAVVHDVLDQTITRGEAALAESATGGDALRCYMHDA
                     IDIGLGAVNVVHALLDDTDWPDRRVAAQDVLDRLVAAARRDGAIDTDVTATDIALATI
                     RFCRPLTIGLDAAEERAIAHRQLTTYLDGLTHRPSGG"
     gene            complement(10127..10528)
                     /locus_tag="VLR27_04795"
     CDS             complement(10127..10528)
                     /locus_tag="VLR27_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP02794.1"
                     /translation="MTIRLHVSSVFVDDQEHALRFYTDVLGFEPRTDVPLGNGDRWLT
                     VGAPGQDVELLLEPSGHPAVKPYREALAEDGIPLASFAVDDVHEVHDRLVQAGVSFTQ
                     PPAEMGPVTTAVFDDTCGNLIQLITRPTDGG"
     gene            complement(10525..10806)
                     /locus_tag="VLR27_04800"
     CDS             complement(10525..10806)
                     /locus_tag="VLR27_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSP02795.1"
                     /translation="MTDVFDALAAPARRAILDVLAERDDQTLFELCARLASDHDIDLT
                     RQAISQHLAVLESAGLVQARRDGRYKFHSIDISPLRGALARWLTEEQHP"
     gene            10906..11184
                     /locus_tag="VLR27_04805"
     CDS             10906..11184
                     /locus_tag="VLR27_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02796.1"
                     /translation="MPAGDRGARCVAIFWILGVVHAAAAAGMWWIPWTGYDSQCSLAT
                     RNDENIRGACADGVAALMPWFVFLAVTASVLLLLGLIIGRRGRRSGSS"
     gene            complement(11195..>11663)
                     /locus_tag="VLR27_04810"
     CDS             complement(11195..>11663)
                     /locus_tag="VLR27_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HSP02797.1"
                     /translation="DDYFTRDFANNIGAEIMGRGKFGPQKGPWEDLEWEGWWGDTPPF
                     HTPVFVLTHHARPTFSLSDTTFHFIDAEPAEALRHAKAAADGKDVRLGGGVSIVRAFL
                     DADLVDTMHVAVAPVEIGRGGKLWDSPDELLDRFHHERIPSPSGVTHHLFWRR"
BASE COUNT         1710 a         4100 c         4219 g         1634 t
ORIGIN      
        1 ccgccaccgg ctcggtgtgc ccctccacgt cgatggtggg caggaggcgg tcgagccagc
       61 cggggagcca ccagttgcgg tcgcccagca gctccatcgt cgccggcacc agcagcatgc
      121 gcacgatggt ggcgtcgagg aagatggcgg tggccaggcc ggtgcccatc agcttcatga
      181 cccggtcggg ttcgaggatg aaggcgccga agaccacgac catgatcgcc gccgcggccg
      241 tgatgacctt ggcggtggcg gcgaggccgt cggccaccga ctcccgggag tcgccggtgc
      301 gcaaccactc ctcccgcacc cgggagagca ggaagacctc gtagtccatg gacagcccga
      361 agacgatggc gaagagcatc atcggcgccc acggctcgat cggtgccggc tgcacccccg
      421 tcaggtcgct gagccacccc cactggaaca gggcgacgac cacgccgtag gccgcgccga
      481 tggagagcag gttcatgatc accgccttga gcggcacgag gagcgaccgg aacacgacca
      541 tcagcagcag gaacgacagc gccaggacgg caccgaagaa gtacggcatc cgctcggtca
      601 ggaactgggc gaagtcgatg ttcaccggca cggtgcccgt caccgcgagg tccacgcccg
      661 cccggtcctc gatgggcggc aggacgtcgt cgcgcagccg gtgcacgagc cgctcggtgg
      721 ccacgtcctg cgggcccgtg gtcggcacga cgttccacag gaccgcggtc ggcgcggacg
      781 ggtcgttcgg gacggcgggc gagacgaacg cgacacccgg atcggcgctc acggcgtcgg
      841 tgatcgaccc gagcgcgccc gggtcgtcgg tgcgggcgac gatcatgaac ggcccgttga
      901 agcccggtcc gaagccctcg accatcaggt cgtaggcctg gcgggtggtg gtggactcct
      961 cgaagttgct ctcgtcggag aagccgaggc gcaggctcag ggtgggtcct gccagcagca
     1021 gcaggatcgc cgcgccggcg atcgccgccg gccacggccg gtgctggatg acccggctcc
     1081 accggtatgc cgcggtctcc cggcgcggct tctgggcacg ggccgggacc tcgcggcgca
     1141 ggaacgggat ccacgtgccg gcgatcagca ccactgcggc gacgggcagg ccgatgacca
     1201 gcggggtgac cttcaggccc atgccggcga gtgccagggc gacgaagccg gctgcgacca
     1261 ggccgcgcca acgggtgcgc tccacccggt cgccggcgaa gccgagcagg gcgggcagga
     1321 gcgtgagcga cgcgaccacc gtgacggcga cgacggaggc ggcgccgacc gcgaggccct
     1381 gcacgaagct gacctgcatc agcagcatgc cgagcagcga gatcaccacg gtggtgccgg
     1441 cgaagagcac ggcccggccg gccgtgtcga gggcgacggc gaccgagtcg gccacggtgt
     1501 gaccctggtg catctgctcc cggaaccgag tgacgatgag cagggcgtag tcgatgccga
     1561 cgccgaggcc gatcatgatc ccgaggaacg tggcgaagtc cgggatcgac agcacgtggc
     1621 tgaggagggt gatgatcgtc gttccgatcc cgatgccgaa cagcgccacg cccacgggca
     1681 ggcccatggc cagcacggac ccgaacgcca ggatcaggac cacgatggcg aaggccaggc
     1741 cgagcagctc ggctgtcggc tcctcgaact cggcgaagat gtagccgccg agctcgagct
     1801 gcaggccgtc gacggtcggt gccgcctcca ggatctcgtc gcggatctcg acggctcgct
     1861 cgaagtcgac gtcctccggc agctcgacgt tcgcgtaggc gatggtgccg tcgctgctga
     1921 tctgccgacc gccctcgggc gcgtaggggc tgtccacccg gtcgacgtcg tcgatctggg
     1981 cgatggcggc gaacaggtcg gtcatgaccg cctggacggt cgcgtcgtcg acgccctggt
     2041 cggctcggaa gacgaccgtg ccggtcatgc ccgtgccctg gccgccgaac tcctcgtcga
     2101 ggatgtcgaa gccgtccttg gactcgacgt cggggaggct gaactcgtcg cggaacgcgc
     2161 tgccggcggc gccggagatg acgccgccga gcacgagcgt ggcgatccac aggccgagca
     2221 cggtgcgccg gtggtcgtgg caccaggtgc cgagtcgtgc gaacaaggtc gtacctcctt
     2281 ggagcgacac ccgcccgggc gtcgcgatcg atgggtcgcc tccgttgcgc cggtcctcgg
     2341 gagacggcgt cggcgcgtcg ggtttcgtcg gtgcagacgt cacgacccgt cgcgaaatca
     2401 tcgctcctgc cgccctgctt ggcgatgtca ttttcggtcg ccggtcatcg catacccgtc
     2461 ccattcggac atgttctgcg tgtgcaaatg acacatttcg ctcggggccc atccgccggg
     2521 acctggcgtc cgtacatggg aggacagaac ccgttccacc gaaaggcatt ccctgatgag
     2581 acgagctctc accgctgccg tcgtggcggc gctcctggtg gccggcccct tggctgcgtc
     2641 ccctgcgggc gccgtcggcg gcaccaccag caccaccgtg gtccacggca tccccgacct
     2701 gaccgtcgac gtctacgtcg acggcgccaa ggccatcgac gacttcgtgc ccggcacggt
     2761 cgccggtccc ctcaccctgc ccgccggcac tcgctccgtc gccatcaccg ccgcggacgc
     2821 gatggacgat tccgatcccg tcctcgccgg tgacatcgtc ctcgttgcgg gcgccgacat
     2881 gtcgatcgtc gcgcacctcg acggtgccgg tgacccgacg gtgtcggcct tcaccaacga
     2941 cacgtccgcc atcgccgagg gcgagggtcg gatcaccgtc cgtcacctcg cggctgcacc
     3001 ggccgtcgac atcctggccg ggggcgccgt cgcgttcgct ggtctgacca acggcgcatc
     3061 tgccgcggcc gacctgccgg ccggcacgct gcccgtgtcg atcaacgccg ccggaacctc
     3121 gacccaggtc ttccccgccg aggggacgct cgacgtggcg ctgcccgccg gcgtgaacct
     3181 ggtcgtctac gcgtggggtg acttcgcagc ggggagcttc gccctcgcga cccagaccat
     3241 cggcgagggc accatcggtg cgccgtcgca ggtctccgtc gtccacggcg tgcccggcct
     3301 gaccgtcgac gtgtacctga acggcgtgct cctcctcgag gacttcgagc cgacgcaggt
     3361 ggccggtccg gtgctgctcc ccgccgggac gtacgacatc gaggtcttcg cggccgacgc
     3421 cgcgtacgcc cccggtggcg gcgccatcac ggtggacgat gcggtcgtgc ctgccggcgc
     3481 caacatctcc gccgtcgcgc acctcgacga ggaaggcgcg ccgaccgcgt cgctcttcgt
     3541 gaacgacacc tcgcccatcg acgacggtga agcccgcctc gtcgtccgcc acaccgcggc
     3601 ggcgccgacg gtcgacgtgc tgaccggcga caccaagctg atcgacagca tcaccaaccc
     3661 ggagtcccag cagctcgacg tgccggccgg cacctacccc gcggccgtcc acgccgagga
     3721 cgaccccgag ccggccgtcg acctcggtga tgtgaccctg cctgccgggc aggcgaccat
     3781 cgtgtacgcc atcggcgagc tgggctccac gttcaccgtg gccgcgcagt cgatcgtcgg
     3841 tctcgccgcc ggcggcgcct tcgctgacac cggcgccgac cagccctcct ccgacgacgt
     3901 gcgcacgctc ctgcagctcg ggatcaccaa cggcacgtcg ccgacgatgt actcgcccag
     3961 cgagtgcgtg acccgtggcc agatggctgc cttcctggtc cgcacgctcg agctgccggc
     4021 gtcggacgtc gatgccttcg gtgacgacga cgggacgttc ttcgaggacg agatcaacgc
     4081 cctcgctgcg gccggcatca ccgccggcac gtcggcgacg acgttctcgc ccgaccagtg
     4141 cgtgacccgc ggccagatgg ccatgttcct ggctcgtggg ttcaacgtgc ccgcctccaa
     4201 cgtcgatgcc ttcggtgacg acgacggcac gacggcggag ccggcgatca acgccatcgc
     4261 cgccgccggc gtcacgatgg gcaccacgcc gaccacgttc tcgcccggcg agcacgtgag
     4321 ccgtggccag atggcgtcgt tcctcgcccg gtccctcggg gtcggctgac gcccgacgac
     4381 acgtcctggc ggggcgtcac cgttcgacgg tggcgcccct ccgggcggtc tccatgtacc
     4441 ccgtgctcag cgtgatccag tgctccatgg ctgcgtcgac ggccgctcgg tcctgggtcg
     4501 ccagcaggtc caggagcagg ccgcgggtca cggcgaggcc gagccgggcg aacgcctccg
     4561 ccgtctccgg cgggacgccc gcagcctcgg cgagcgctgc cgccgggcgg acccagggct
     4621 cgaccacgcc gtccagcagc tcgttggccg gaggacggtc gtgcagcgcc tgggcgtaga
     4681 cctcgaagaa caagcgctcg ttgggccaga gcgccggagc ggagatgtgc tgccaccacg
     4741 tgcgcatcag gtcatcgagc ggcagcgacg gatccgggtc cagctcggtg aacgcctcga
     4801 gctgacgtcg ctcgacctcc ctcacgatgg cgagccacag gccgtccttg gagccgaagt
     4861 ggtggctgag catgcgatgg ctcgtgccga gctccgtggc gaggctccgc agcgagaggt
     4921 cggcgagacc gtgctccgcg acgtggtcga tggcggcgtc gagcagccgt tggcgggccg
     4981 atccccgggt catccggaaa actgtaccac ttggttcacg atgcatgtac catctggtac
     5041 atgctgaagc ctgtgaccgt ctccatcgac gtcgaccacg acatcgagga cgtgttcgcg
     5101 ttcctcgacg tcatggccaa ccacgaaccg ttcaacgacc acctgatgac cgactgggag
     5161 ctgtccggtc ccgagcgggg cgtcggatcc aaggcgcgcg tgcggaccag ggcgctcggc
     5221 atgaccgacg cggtcgacat cgaggtgatc gaagcccaag cgccgaccac gatcgtcgag
     5281 cgcaacgtcg cggccaaggc cgggcgcacc ggcgagggca cctacacgct gagccggctc
     5341 gatggtggcg ggacgcggat caccttcgag taccggtgga tcgtcacccc ggtcctcgac
     5401 cggatcacca agccgatcgc acgcgcctac atccggcgca acaacaccac gtccatgcgt
     5461 cgcctggccc gccagctcga cctcgagctg gcggcgtgat cgtctggtcg aggagcgcgg
     5521 tcgttgccgc ccgccccccg atctcacgcg gcgtcgggtc cgacgccggc gaggacccgg
     5581 tcgatgacgg cgcgggtgga ctgcacaacc gtgtcggcgc ccatcgccgc tccccgggcc
     5641 tcggcctcgg cgtagcgggt gtggccgagc gcgctcggca gctgctccag gatcgccccg
     5701 acgcgacgga ggtccgaggc ctcgatgggc atggccagct cggcggacgc gagccggacc
     5761 gccgcgtgca gcatcgctgc cgcctcgtgg tcgccgcgct gggcgaggac gccggcgacg
     5821 tggcgcaggg tcagccactg gttggcccag tcaccggccc ggtgccacat gtcgatgacc
     5881 tcgcgggcga ggcggagcgc accgtcgagg tcgccccggc ggccggcgag ggacacgagc
     5941 tcggtctggg cgaaggcgac gatccagcgg ttgccggccg ccgtgccgtg ggcgatcgac
     6001 tcctcgagca tggaggtggc gcggtcgggg tcgatcgcct ccagggtcag ggcgcgggcg
     6061 tagagcgccg acgccaacga ggtcgggttc ccgatccgcc gggccaggct gacggcttcg
     6121 tcggccagca agcggctggc ctcggtccgg gcatcgctgg ccaggccgac cgacgccatg
     6181 tagagcgcgt gggccaggcg ggcgtcgtca ccgctggccc gggccgcgcc gaccgtgcgc
     6241 tggcaggtct cggcggcctc gaaggtctgc ccccggtagt agtagacgtt tcccagcgtc
     6301 cgcgagtgga ggccgcagga cgggagcccc agccgctcct cgacctcgag cgaccgctcg
     6361 gcgagcgccg tcgcggcgtc gaggtccccc cggacgaacc ggccgtaggc ggcggtggcg
     6421 agggccatgg gcaggagcgg gtggtcgtcg gtggtgagca gctcgagggt cgcctcggcc
     6481 cagctgaaca gctcgtaccg catccggcgg aaggcgaact cgcgggggcc gacgaccagg
     6541 cgcagcgccc cgtcctcgtc gccctgggtc agcatccagc ggtgggcgat gcgcaggtcg
     6601 gcgaaggtgt cgtcgagcct cgacatccac accggctcgt ccggcccgcc cagaccgacc
     6661 tcggcgtgct cgacgaggtc gatcgcccag agggcgtggc gctcctgcag cacgtcggcg
     6721 cacccggcga gccggagctg cgcgccaccg aactgccgga gcgtctccag cagggagtag
     6781 cgggtgggtg agcggctgat gtcggcgacg accatggact tgtcgacgag cgcggccagc
     6841 agcccccgga tgccctcggc cgagaccgtg gcgtcggcgc acacgtactc gatgtcgtcc
     6901 acggtgaagc tgccggcgaa gaccgacaac cggtcgaaga gcgcctgctc ggcgacgccg
     6961 agcagctggt aggaccaggc gacggttgcc tcgagcgtct ggtgccgtgg gtcgccggcg
     7021 ggggtgacga ggatccggaa gtcgccggcc agctcgtcgg ccaggtcggg cagggtcatc
     7081 gagcgcacgc gggcggcagc gagctccagt gcgaggggga ggccgtcgag gcgacggcag
     7141 atctcgacga cggacgggac ggtcgcctcg tcgagccgga agcccgggtg ggcggacgtg
     7201 gcccgggcca cgaacagccg caccgccggg gactccatcg ccatgaccgg gtcggtctcg
     7261 tccgcacggg ggagcgggag cgggccgagc ggccagaccg tctcaccctc gacgcccagg
     7321 ggctcccggc tggtcgcgac gatctggacc ccggcgcacg cccgcagcac ggcgtcggcg
     7381 acctcgcgga tgggcgccag gacgtgctcg cagttgtcga gcacgatgag caggcggcgg
     7441 gactggagca cctcgacgag gccctcgatg gtcgtgcggt cctgacggcg ctgcacgtcc
     7501 agggcggtgg tcacggcctc ggcgacgagg gcgtcctcgc ggacgggggc gaggtcgcac
     7561 agccacacgc cgtcggggtg ctcggtcgca gcgcgctcgc cgagctgcat ggcgaggcgc
     7621 gtcttgccga cgccccccgg cccggtgagg gtcaggaggg gaacggtgcc gagcacctcg
     7681 gtgagctgcg agagctcgac gtcccgaccg agcagggcgg tgacccggcg cggcaggttg
     7741 ccccgcggtt ccccatcagc cggcgccggt ggcggtggac cactcggcgg ggcgaccggt
     7801 ggcgcatcca gcgtcgggtc cgtgcggacc atgcgcgcct cgaggtcggc gagcgcctcg
     7861 gacggctcca ggccgagctg gtcaccgagg tcggagcgga accggtggaa ggcgcgcagc
     7921 gcctcgggct gccggccgga tcggtacagc gccagcatct gctgggcccg gaacccctcg
     7981 cggagcggcc agctggcgat gcaggtctcg agctcgtcga tcgcgccggc gtggtcggcg
     8041 agcgccagca gggcatccac gcgggcttcg gcggcgcgct gacgcacctc ctccaggcgg
     8101 gtgctctcgg cctggagtcc cagatcgtcg acgaactcgc cgaaggcccg gccgccggac
     8161 caccaaccca gcgcctcctt cagcaggtcc gccgcccgct gcggctcccg accgaagacc
     8221 ccgagcgcgc tggcgacctg gcgctcgaag cggacgctgt cgagctcgtc ggggtcgatg
     8281 gtgagctggt agccgcctgg ccgcgtctgg agccgggagc cgtcgtcgcc gaggtcgccg
     8341 cgcaaccggg acacgtagct ctggaccgtg cccgccgccc gctccggggg gtcctggccg
     8401 aacagcacgt ccacgagctg gtcgatgccg caggtcgcgt tgcggtgcag gaccagggtg
     8461 gcgagcagga gccggcgacg agcaccgcgg acggcacagg gggtgccgtc gcccgagatc
     8521 acctcgaggg gtccgagcac ccggatctgc atcgccccgc cccccttcgt catggagaca
     8581 gtacgacgca tccaggttgc agtgcgcatg catgtgcgct gcacgggcgt cagtggaacg
     8641 tgggcgtcgg cgaggcaccc ggcccgccca caacgaggag aacaccatgc cgttcgtcaa
     8701 cgtcaagctc atcgaaggcg tcttcagcgc cgaccagaag cgcgacatga tcgccaagct
     8761 caccgacacc atggtcgcca tcgagggcga gaacatgcgg ggcgtcacgt gggtcgtgct
     8821 cgaggaggtc accagcggcg agtggggcat cggcgggcag gcgctcagca ccgccgacgt
     8881 gaaggcgctc gccgccggcg cgccggcctg agcgcacgcc acccctcgcc gaaccgggcc
     8941 gcggtgcacg tatcaagatg cgtgcaccgc ggctccggaa cggtgcgtgc gcgctagtcc
     9001 tcgatgacgt ccagtccctg ctcgcgcagc cgagtgaggt tgtcgagcat gccctgcagc
     9061 acctcggggg gatgcccgtc ccagctctcg acctcgctga cgaccagcat cgggtgccgg
     9121 gtccggtacg accgcgtcac gttgccgggg aaccgcttgt tcgtgacgtt ggggtcgtcc
     9181 tcgaaggggc cggtgggctc gacgacgtag atgcggcccc ggtcatcgct gcctgcgagc
     9241 gcgttcgcca gctcggccgc ccaaaccgca ggctcgagca gcgcggcgaa gtagacgtgg
     9301 ttcgaggtcc gaccgtcctg gtagttggag gggtggccgg ggacgatctc atcgccgacc
     9361 tcgaacgtcg tcctcgtccc gtggaagaac gggccgtcga cgtggtcgca gcactcgaac
     9421 gtcacgggga cgtgctcggt gtcgtgcgac gcggcgatgg cgtcggcgat cgatctggtt
     9481 gcgtgcgtgg gttcctcgtc gggcaccgcg tcctccccgg gggatgtcac ctacaagcgg
     9541 aggaatatac tccgattcgt gcgcgacgtc accccttcgg acaccgatcg acccatgcgg
     9601 gtcgacgcgc gtcggaaccg gcgccgcctc ctcgatgccg ccatcggcct gatcctcgag
     9661 atcggcggcg agccgtcccg ggacgccgtc gcccagcggg ccggcgtcgg catcggcacc
     9721 ctctaccgcc acttccccga ccggcagtcg cttctccacg cggtggtcca cgacgtgctc
     9781 gaccagacga tcacccgggg cgaggccgcc ctcgccgaat cagcgaccgg tggcgatgcg
     9841 ctgcggtgct acatgcacga cgccatcgac atcggcctcg gggcggtcaa cgtcgtccac
     9901 gccctgctcg acgacacgga ctggcccgac cgtcgcgtcg ccgcccagga cgtcctcgac
     9961 cgactggtcg ccgccgcccg acgggacggc gccatcgaca ccgacgtcac cgcgaccgac
    10021 atcgccctcg ccaccatccg gttctgccga ccgctgacga tcggcctcga cgccgcggag
    10081 gagcgggcga tcgcccaccg tcagctcacc acctacctcg acgggctcac ccaccgtccg
    10141 tcgggcgggt gatcagctgg atcaggttcc cgcacgtgtc gtcgaagacc gcggtcgtca
    10201 ccggccccat ctccgccggt ggctgggtga acgacacgcc ggcctggacg agccggtcgt
    10261 gcacctcgtg gacgtcgtcc acggcgaagg aggcgagcgg gatgccgtcc tcggccagcg
    10321 cctcccggta cggcttgacg gcggggtgtc cggagggctc gagcagcagc tcgacgtcct
    10381 gtccgggtgc accgacggtg agccaccggt cgccgttgcc gagcgggaca tcggtgcgcg
    10441 gttcgaagcc gaggacgtcg gtgtagaagc ggagcgcgtg ctcctggtcg tcgacgaaga
    10501 cgctgctgac gtggaggcgg atggtcatgg gtgttgctcc tcggtgagcc atcgggccag
    10561 cgcaccgcgg agcggtgaga tgtcgatgga gtggaacttg tagcgaccgt cgcgtcgtgc
    10621 ctgcaccaga ccggccgatt cgaggacggc gaggtgctgg gagatcgcct ggcgggtcag
    10681 atcgatgtcg tgatctgacg cgaggcgagc gcagagctcg aacagcgtct ggtcgtcgcg
    10741 ctcggccagg acgtccagga tcgcccgtcg tgccggtgcg gcgagggcgt cgaacacgtc
    10801 ggtcacgtgc cgcaccatag gcaagttgac gcttgcatgc aagtggagac ttgcatgcgg
    10861 ttgtggacgt cctgccatgg cgggcggcga cgtatcgtct cgacgatgcc ggccggcgac
    10921 cgaggcgctc gatgtgtcgc gatcttctgg atcctcggcg tcgtgcacgc agctgctgca
    10981 gccggcatgt ggtggatccc gtggaccggc tacgactcgc agtgctcgct cgcgacccgc
    11041 aacgacgaga acatcagggg cgcgtgcgcc gatggggtgg ccgcgctgat gccgtggttc
    11101 gtcttcctcg ccgtcaccgc ctcggtcctg ctcctgctcg gcctcatcat cggccggagg
    11161 ggccggcggt cgggatccag ctgaggcgcc cgggtcagcg acgccagaag aggtggtggg
    11221 tcacgccgct cgggctcggg atccgttcgt ggtggaagcg gtcgagcagc tcgtcggggg
    11281 agtcccagag cttgccgccg cgcccgatct cgacgggggc gacggcgacg tgcatggtgt
    11341 cgacgaggtc tgcgtcgagg aacgcacgga cgatggacac gccaccgccg aggcgcacgt
    11401 ccttgccgtc ggcggcggcc ttcgcgtggc gcagggcttc ggccggctcg gcgtcgatga
    11461 agtggaaggt cgtgtccgac agcgagaagg tgggtcgggc gtggtgggtc agcacgaaga
    11521 ccggtgtgtg gaacggcggc gtgtcgcccc accagccctc ccactcgagg tcttcccacg
    11581 gtcccttctg cgggccgaac ttgccccggc ccatgatctc tgcgccgatg ttgttggcga
    11641 agtcgcgagt gaagtagtcg tcg
//
LOCUS       DATISU010000117         9262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_750498, whole genome shotgun sequence.
ACCESSION   DATISU010000117 DATISU010000000
VERSION     DATISU010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9262
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750498"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="VLR27_04815"
     CDS             <1..1497
                     /locus_tag="VLR27_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HSP02798.1"
                     /translation="RTGDLARIDQDGVVEIVGRLRQFVKVFGLRLDLRRLEDLLGDEG
                     IRAECLGDDSGLAVAVLDDHDPTAIRALLAERTGLPAAAIAVARIADVPRLANGKLDA
                     VALTETVRVADRPAPAGSADSVAAVLGRLLGTTPGPGDTFVSLGGDSLTYVEASIALE
                     ERLGHLPHDWHLRPIEDLDVDDGSARRGTRIETGVLLRAIAIVLVVGNHAGAFMVSGG
                     AHVLFAAVGFNMARFQLTAGTWGRSLARILVPSVIWIGGAALVQDRFHLSHALLVHGL
                     VDGSGRWIYWFIEALVQMLVVTALAMRVPAIRRFEQRRPLGFPALLLVPALAVRFDLV
                     ELGPERFAVFRPHEIAWIFLLGWMAARSRTALHRAAVTTVALAAVPGFFGNPSREAVV
                     LAGMLLLVWVPTIRVPRWLPRLAGPLASGSLYIYLTHAQVYPPLRERWPVLALVASLA
                     VGMAIWRAIQPLQQRIEQGAPRRGPTSSDAGSPGASPPRPAADRVPSR"
     gene            complement(1424..2155)
                     /locus_tag="VLR27_04820"
     CDS             complement(1424..2155)
                     /locus_tag="VLR27_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02799.1"
                     /translation="MDRRADIHFHFDPVCPFAWLTSRWVRSVARQRDYEVDWRLISLR
                     LLNEHIDYDAHFPPEYEQGHTAGLRMLRVCAAARERHGREVVGDLYTALGGSVFDVDP
                     PTDKAAYRDGMGARETVAPLLAGLDLDTDLVDALDDPAWDEVVRQETDEALARTGRDV
                     GTPIIAYDPPDGPAFFGPVISRVPDDADALRLWDAVLELTSFPGFAELKRSLRERPAL
                     RVSGTAPDQPPVEEDWHQGSRRLKK"
     gene            2199..3560
                     /locus_tag="VLR27_04825"
     CDS             2199..3560
                     /locus_tag="VLR27_04825"
                     /EC_number="1.8.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycothione reductase"
                     /protein_id="HSP02800.1"
                     /translation="MQDFDLVIVGSGSGNSIPAELDGARIALVERGVFGGTCLNVGCI
                     PSKMFIYAADIAMHVRDSERYGVHASVDSVDWPSIVERVFGRIDPIAAGGERYRIEDC
                     ENITVFQGSGAFVDHKVLEVDGQQLRGEKFLLAAGARPYIPDVPGLDDVGYHTSDTVM
                     RLPELPERMAVLGGGYIATELGHVFDAFGTRVSIINRSGSLLRSEDDDISRRYTELAA
                     GRFDLHLDTAVTKVERTGSGSILVHLLHDGQESTLEVDELLVATGRVPNSDQLAVERT
                     GVRTDPSGRVVVHEDLQTDVEGIYALGDITNHYQLKHLANAEARVAFHNLVHPDDPWR
                     MDYSIVPHAVFGSPQVASVGMTEHEAATDGVPFVTAIKEYGSTAYGWAMEDTTSFVKL
                     VAHAQTRRLLGAHIIGPQASTLIQVLIQGMRFGQTVDDMAREQMWIHPALTEVLENAL
                     LDL"
     gene            complement(3561..4604)
                     /locus_tag="VLR27_04830"
     CDS             complement(3561..4604)
                     /locus_tag="VLR27_04830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSP02801.1"
                     /translation="MEHIELGIDTFGDVTVGADGQALAHPQVIRNVVEEAVVADQVGL
                     DFFGLGEHHREDFAVSAPDVVLATIAGRTERIRLGSAVTVLSSDDPVRVYQRFATLDA
                     LSGGRAEVILGRGSFTESFPLFGYELSDYEVLFEEKLELFAALLEEEPVTWSGTTRAG
                     LRDQRVFPTTDGGLRAWVGVGGSPESVVRTARHGLPMMLAIIGGDPLRFAPFVDLYRR
                     ALDELDRPQLPIGIHSPGHVGETDEQAREELWPHYREMHARIGRDRGWPPMTRSQFDL
                     AAGPEGALAVGSPATVAGKIVRAVEGLGASRFDLKYSAGTLPHESMLRSIELYGTEVA
                     PLVREQLARTEAA"
     gene            complement(4676..5164)
                     /locus_tag="VLR27_04835"
     CDS             complement(4676..5164)
                     /locus_tag="VLR27_04835"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HSP02802.1"
                     /translation="MPTSQLFTFPDPVNEVSARLVATGVVTMSVAAIALDLRWLVAVI
                     AYGFVARVLTGPTLSPLGQLVTRVLTPALDRPAKLVPGPPKRFAQGIGVAFSVTALVL
                     VLADQWGAAQVVLGMLATAALLEAAFALCLGCKAFALLMRAGVIPDDVCERCNDIWAT
                     SS"
     gene            complement(5235..6605)
                     /locus_tag="VLR27_04840"
     CDS             complement(5235..6605)
                     /locus_tag="VLR27_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HSP02803.1"
                     /translation="MLTVIDPTTGSELHTLPAADRDQLDATLGRAHDAFDGWRRRSFA
                     ERAEVLRAVATRMRDDIERLAPLMTEEMGKPITEARGEVNKAAWAAEHYAEHAEEYLA
                     TDVIESDATSSYVQYLPIGPVLGILPWNAPFWLAFRFCAPALMAGNTCVMKHDPHVPA
                     TAAAIAEVFEAVGAPAGVFQNLPLQTDDVATAIADPRIRAVSFTGSERAGSAVASAAA
                     AELKPAVLELGGSDPAIVLADADLEAAAAAIATMRIINAGQSCIAAKRIIVEDGVHDR
                     FVDLLHERLAALVVGDPREETTQIGPIARDDLRENLHRQVRQTIDAGATCRLGGELPE
                     GDGFFYPVTLLTDVKPGMTACIEETFGPVAAVLRVGSADEAMALANDTPYGLAASVWT
                     TTERGEAMAADIEAGQVAVNGVVKTDPRLPSGGVKRSGYGRELGPHGIHEFVNAQQVW
                     VGPSTA"
     gene            complement(6599..7783)
                     /gene="doeA"
                     /locus_tag="VLR27_04845"
     CDS             complement(6599..7783)
                     /gene="doeA"
                     /locus_tag="VLR27_04845"
                     /EC_number="3.5.4.44"
                     /inference="COORDINATES: protein motif:HMM:NF038259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine hydrolase"
                     /protein_id="HSP02804.1"
                     /translation="MRARDDMLFPFEEYERRIRELRERMEERLLDAVVITAPGNLMYL
                     TDYQTTGYSFFQALVVPLDGEPFTITRALEESNVIERTWVELTRTYTDTGDAIQLLTE
                     SMREFGLEGKAIGYERNSYFFPAYQQDRMADAFRDGRLQDCYGIVEEGRITKSPLEID
                     VMKRAAKATEAGMRAGLAACQAGVTENEIAAEVSAAMFRAGGEFPAVMPYITSGPRTM
                     IGHATWEGRTVQPGEHVFIEVGGCYRRYHTAMMRTAVVGELSDSMRRAQDRMKLCIEE
                     MHATVRPGMTISDFDQLVRSIITANEIGAELVTRAGYSIGIAFPPSWDEGYIQSIKPG
                     DNTVLQEGMTFHVIPWMWGVDGDKTVGISDTIRVTADGIASFFTLDQDFTVATRPEEL
                     PC"
     gene            complement(7780..9042)
                     /locus_tag="VLR27_04850"
     CDS             complement(7780..9042)
                     /locus_tag="VLR27_04850"
                     /inference="COORDINATES: protein motif:HMM:NF006733.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HSP02805.1"
                     /translation="MNNEAFEQWESAIRGYCRAYPTVFTSAKNARMHDEDGRSYLDFF
                     AGAGVLNFGHNDERMKRAMIEFLERDGVAHSLDTHTDVKRDFIERFAEVVLEPRGMDH
                     KMQFMGPTGTNAVEAALKLARKVTGRPHVVGFSHGFHGMTLGSLALTANDVFRQAAGV
                     PLEHVVRLPFETAAGGGLKGLESYRAALEDSSSGLLPPAAFVVEVIQAEGGVNVASPE
                     WLHAVQDLARDVGALFIIDDIQAGCGRTGSYFSFDGMDLDPDIVTLAKGIGGFGTPLA
                     MNLNKPEHDQHWSPGEHTGTFRGQGLSFVAGTEALGYFTDDTLMNQVAEKGEVMRQRL
                     RSLADARADHGWDVRGRGMMQAVDVVDGAYAKDVQRECFERGLLIGPCGTGGRVIKLI
                     PPLTIPDDELTEGLDILEAAIDATWSAT"
     gene            complement(9059..>9262)
                     /locus_tag="VLR27_04855"
     CDS             complement(9059..>9262)
                     /locus_tag="VLR27_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HSP02806.1"
                     /translation="PVPIMASEDFSYYLTEVPGAFALVGADDGQGHDASCHSPHYDFN
                     DRLIGPVVRVYARLAGAPLPQPT"
BASE COUNT         1295 a         3253 c         3321 g         1393 t
ORIGIN      
        1 cgcaccggcg acctggcccg gatcgaccag gacggggtgg tggagatcgt cgggcggctg
       61 cgtcagttcg tgaaggtgtt cgggctgcgc ctcgacctgc ggcgcctcga ggacctgctc
      121 ggcgacgagg gcatccgggc cgagtgcctc ggggacgaca gcggcctggc ggtcgccgtc
      181 ctcgacgacc acgaccccac ggccatccga gcgctgctcg ccgagcgcac cgggctccct
      241 gcggcggcca tcgccgtcgc ccgcatcgcc gacgtgccgc ggctggccaa cggcaagctc
      301 gatgccgttg ccctcaccga gaccgtccgt gtcgcggacc ggcctgctcc ggcgggcagc
      361 gccgactccg tcgccgccgt gctcggccgg ctgctcggca ccacgcccgg acccggagac
      421 acgttcgtct ccctcggtgg cgactcgctg acctacgtcg aggcgtcgat cgccctcgag
      481 gagcgcctcg gccacctccc ccacgactgg cacctccgac ccatcgagga cctcgacgtc
      541 gacgacggct ccgctcgacg gggcacccgc atcgagaccg gcgtgctgct gcgggcgatc
      601 gccatcgtcc tcgtcgtcgg caaccacgcc ggcgcgttca tggtgtccgg cggcgcccac
      661 gtcctgttcg cggccgtcgg gttcaacatg gcccggttcc agctcaccgc cggcacgtgg
      721 ggacgcagcc tcgcccgcat cctggtcccg tccgtgatct ggatcggtgg tgccgcgctg
      781 gtccaggacc gcttccacct cagccacgcg ctcctcgtgc acggcctcgt cgacggcagc
      841 ggacggtgga tctactggtt catcgaggcc ctggtgcaga tgctcgtcgt caccgccctc
      901 gccatgcgcg tgcctgccat ccggcgcttc gagcagcgcc ggccgctcgg gttcccagcc
      961 ctgctgctgg tgccggcgct ggcggtgcgc ttcgacctgg tggagctggg acccgagcgc
     1021 ttcgccgtct tccgccccca cgagatcgcc tggatcttcc tgctcgggtg gatggcggct
     1081 cggtcccgca ccgcgctgca ccgggctgcc gtcaccaccg tcgcgctcgc cgccgtcccg
     1141 gggttcttcg gcaacccgag tcgcgaggcg gtggtgctcg ccggaatgct gctgctcgtc
     1201 tgggtcccga ccatccgggt gccgcggtgg ctcccccgcc tcgccggccc cctggccagc
     1261 ggatcgctgt acatctacct cacccacgcc caggtctacc cgcccctgag ggagcgttgg
     1321 cccgtgctgg cgctggtcgc gtcgctcgca gtcggcatgg cgatctggcg ggccatccag
     1381 ccgctgcagc agcgcatcga gcagggcgct ccgcgccgag gacctacttc ttcagacgcc
     1441 ggctcccctg gtgccagtcc tcctcgaccg gcggctgatc gggtgccgtc ccgctgaccc
     1501 gcagcgcggg acgctcccgc aggctccgct tcagctcggc gaagcccggg aaggacgtga
     1561 gctccaggac cgcgtcccag agccgcaggg catccgcgtc gtccgggact cggctgatga
     1621 ccggtccgaa gaacgccggc ccgtcgggcg ggtcgtaggc gatgatcggc gtcccgacgt
     1681 cgcgaccggt gcgggccagc gcctcgtcgg tctcctgacg gaccacctcg tcccacgccg
     1741 ggtcatcgag cgcgtcgacg agatcggtgt cgaggtcgag gccggccagc agcggcgcga
     1801 ccgtctcacg tgctcccatg ccgtcccggt acgccgcctt gtccgtgggc gggtcgacgt
     1861 cgaacacgct cccgccgagt gcggtgtaga ggtcgcccac gacctcccgg ccgtggcgct
     1921 cgcgggccgc ggcgcacacc cgcagcatcc ggaggccggc ggtgtgaccc tgctcgtact
     1981 cgggtgggaa gtgggcgtcg tagtcgatgt gctcgttgag caggcgcagg gagatgaggc
     2041 gccagtcgac ctcgtagtcg cgctggcggg ccacgctgcg cacccagcgc gacgtcagcc
     2101 aggcgaacgg gcacaccggg tcgaagtgga agtggatgtc ggcgcggcgg tccatgcggc
     2161 gcagggtagg agcggttctg acctggcagg ctcggcccgt gcaggacttc gacctcgtca
     2221 tcgtcggcag cggctcgggc aactccatcc ccgcggagct cgacggcgcc cgcatcgccc
     2281 tcgtcgagcg cggcgtcttc ggcggcacgt gcctcaacgt cgggtgcatc ccctcgaaga
     2341 tgttcatcta cgccgccgac atcgccatgc acgtccggga ctccgagcgc tacggcgtgc
     2401 acgcctcggt cgactcggtg gactggccgt cgatcgtcga gcgggtcttc ggacgcatcg
     2461 acccgatcgc ggccggcggc gagcgctacc ggatcgagga ctgcgagaac atcaccgtct
     2521 tccagggcag cggcgccttc gtcgaccaca aggtcctcga ggtcgacggc cagcagctgc
     2581 ggggcgagaa gttcctgctc gccgccgggg cccggcccta catccccgac gtgcccggcc
     2641 tcgacgacgt cggctaccac acgtccgaca cggtgatgcg cctgccggag ctgcccgagc
     2701 gcatggcggt gctcggcggt ggctacatcg ccaccgagct cgggcacgtg ttcgacgcct
     2761 tcggcacccg cgtctcgatc atcaaccgca gcgggagcct gctccgctcc gaggacgacg
     2821 acatctcccg gcgctacacc gagctcgccg ccggccgctt cgacctccac ctcgacacgg
     2881 cggtgaccaa ggtcgagcgc accgggtccg gatcgatcct cgtccacctc ctgcacgacg
     2941 ggcaggagtc gacgctcgag gtcgacgagc tgctcgtggc gacgggccga gtgcccaaca
     3001 gcgaccagct cgcggtggag cgcacgggcg tccgcaccga tccgtccggg cgggtcgtcg
     3061 tgcacgagga cctgcagacc gacgtggagg gcatctacgc cctgggcgac atcaccaacc
     3121 actaccagct gaagcacctc gccaacgccg aggcccgggt cgcgttccac aacctggtcc
     3181 accccgacga cccgtggcgc atggactact cgatcgtccc ccacgccgtg ttcgggagcc
     3241 cgcaggtggc gtcggtcggc atgaccgagc acgaggcagc gaccgacggc gtgccgttcg
     3301 tgaccgcgat caaggagtac ggctccacgg cgtacggctg ggcgatggag gacaccacct
     3361 cgttcgtgaa gctggtggcc cacgcccaga cccgtcgact cctgggggcc cacatcatcg
     3421 gcccccaggc gtcgacgttg atccaggtgc tcatccaggg catgcgcttc ggccagacgg
     3481 tggacgacat ggcgcgcgag cagatgtgga tccaccccgc cctcaccgag gtgctggaga
     3541 acgccctgct cgacctgtga tcaggccgct tcggtgcggg ccagctgctc gcggacgagc
     3601 ggcgcgacct cggtgccgta cagctcgatg ctgcggagca tggactcgtg cggcagcgtg
     3661 ccagcgctgt acttgaggtc gaagcgcgac gccccgaggc cctcgaccgc tcgcacgatc
     3721 ttgccggcga cggtcgccgg cgaaccgacg gcgagcgcac cctccggacc ggcggccagg
     3781 tcgaactggg agcgggtcat gggcggccac ccccggtcac gaccgatgcg ggcgtgcatc
     3841 tcccggtagt gcggccacag ctcctcgcgg gcctgctcgt cggtctcgcc gacgtggccc
     3901 gggctgtgga tcccgatggg cagctgcggc cggtcgagct cgtccagcgc tcgccggtag
     3961 agatcgacga agggagcgaa gcgcagcggg tcgccaccga tgatggccag catcatgggg
     4021 aggccgtggc gtgcggtgcg cacgaccgac tcggggctgc caccgacacc gacccacgcc
     4081 cgaaggccgc cgtcggtggt cgggaagacc cgctggtcgc ggagcccggc ccgagtggtg
     4141 cccgaccacg tgaccggctc ctcctcgagc agggcggcga agagctccag cttctcctcg
     4201 aagagcacct cgtagtcgct cagctcatag ccgaacagcg ggaaggactc ggtgaacgag
     4261 ccacgtccga ggatgacctc ggcccggcca ccggacagcg cgtcgagcgt ggcgaagcgc
     4321 tggtacaccc ggaccggatc gtcggagctg aggaccgtga cggcggaacc gaggcggatg
     4381 cgctcggtcc tcccggcgat ggtggccagc accacgtccg gtgcggagac ggcgaagtcc
     4441 tcgcggtggt gctcgccgag gccgaagaag tcgaggccga cctggtcggc gaccacggcc
     4501 tcctcgacga cgttgcggat cacctgcggg tgggccagtg cctgcccgtc ggcgccgacc
     4561 gtgacgtcgc cgaaggtgtc gatgccgagc tcgatgtgct ccatggtgat ctccgtttct
     4621 cgaagggttc accacgtcca acatcgttgc gcgtgcaact attccgcggt ggggttcagc
     4681 tgctggtcgc ccagatgtcg ttgcaccgct cgcacacgtc gtcggggatg accccggccc
     4741 gcatgagcag cgcgaacgcc ttgcagccga ggcagagcgc gaaggccgcc tcgaggagcg
     4801 ccgccgtggc cagcatgccg agcacgacct gggccgcgcc ccactggtcg gcgaggacca
     4861 gcacgagcgc ggtcacggag aaggccacgc cgatgccctg ggcgaagcgc ttcggcggcc
     4921 cgggcacgag cttggcggga cggtccagcg ccggggtcag gacccgggtg acgagctggc
     4981 cgagcgggct gagggtcggg ccggtgagca cccgggccac gaagccgtac gcgatgaccg
     5041 ccacgagcca ccgcaggtcg agggcgatgg cggcgaccga catcgtgacg accccggtcg
     5101 cgacgaggcg ggcggagacc tcgttgacgg gatcggggaa ggtgaacagc tgcgaggtcg
     5161 gcatcagggt cggctccggg gtgggggtgc tgtgtgcaac acccccaccc cggccgatct
     5221 tccggccggg cgcctcaggc ggtggaggga ccgacccaga cctgctgggc gttcacgaac
     5281 tcgtggatcc cgtgggggcc gagctcgcgc ccgtaaccgg agcgcttgac gccacccgac
     5341 gggaggcgag gatcggtctt gaccacgccg ttcacggcga cctggccggc ctcgatgtcg
     5401 gcggccatgg cctcgccccg ctcggtcgtg gtccacaccg acgccgccaa gccgtagggc
     5461 gtgtcgttgg cgagggccat ggcctcatcg gctgagccga cccgcagcac cgcggccacc
     5521 ggaccgaacg tctcctcgat gcacgcggtc atgcccggct tcacgtccgt gagcaaggtc
     5581 accgggtaga agaagccgtc gccttcgggg agctcgccgc cgagccggca cgtggcgccg
     5641 gcgtcgatgg tctggcggac ctggcggtgc aggttctcgc gcaggtcgtc gcgggcgatc
     5701 gggccgatct gggtcgtctc ttcgcggggg tcgcccacca ccagcgcggc gagccgctcg
     5761 tgcagcaggt cgacgaagcg gtcgtggacg ccgtcctcga cgatgatccg cttggcggcg
     5821 atgcacgact ggccggcgtt gatgatccgc atcgtggcga tcgccgcagc cgccgcctcg
     5881 aggtcggcat cggcgagcac gatcgccgga tcggacccgc ccagctcgag cacggcgggc
     5941 ttcagctcgg ccgcggctgc cgaggcgacc gcagagccgg ctcgctccga gccggtgaag
     6001 gacacggccc ggatccgggg gtcggcgatg gcggtcgcca cgtcgtcggt ctgcaggggc
     6061 aggttctgga acaccccggc cggggcgccg accgcctcga acacctcggc gatcgccgca
     6121 gcggtggcgg gcacgtgggg gtcgtgcttc atcacgcacg tgttgccggc catgagcgcc
     6181 ggcgcgcaga accggaacgc cagccagaac ggcgcgttcc acggcaggat cccgagcacc
     6241 gggccgatgg gcaggtactg cacgtagctg gacgtggcat cggactcgat gacgtcggtg
     6301 gccaggtact cctcggcgtg ctcggcgtag tgctcagccg cccacgccgc cttgttcacc
     6361 tcgccccgcg cctcggtgat cggcttgccc atctcctcgg tcatgagcgg cgccagccgc
     6421 tcgatgtcgt cgcgcatgcg ggtggcgacg gcgcgcagca cctcggcccg ctcggcgaag
     6481 ctccgccggc gccagccgtc gaacgcatcg tgggctcggc cgagggtggc gtcgagctgg
     6541 tcgcgatcgg cggcgggaag ggtgtgcagc tcggaccccg tcgtcgggtc gatgacggtc
     6601 agcatgggag ctcctctgga cgggtggcga cggtgaagtc ctggtcgagg gtgaagaacg
     6661 acgcgatgcc gtcggccgtg acccgaatcg tgtcggagat gccgacggtc ttgtcgccgt
     6721 cgacgcccca catccacggg atgacgtgga aggtcatgcc ctcctgcagg acggtgttgt
     6781 cgccgggctt gatggactgg atgtagccct cgtcccagct cggggggaag gcgatgccga
     6841 tggagtagcc ggcgcgggtc acgagctcgg cgccgatctc gttggccgtg atgatcgagc
     6901 ggaccagctg gtcgaagtcg gagatggtca tccccggccg caccgttgcg tgcatctcct
     6961 cgatgcacag cttcatgcgg tcctgcgccc ggcgcatgga gtcgctgagc tcgccgacga
     7021 cggcggtccg catcatggcc gtgtggtagc ggcggtagca gccgccgacc tcgatgaaca
     7081 cgtgctcccc gggctggacc gtgcggccct cccaggtggc gtggccgatc atggtgcggg
     7141 ggccggaggt gatgtagggc atcacggccg ggaactcgcc gccggctcgg aacatcgccg
     7201 cgctcacctc ggcggcgatc tcgttctcgg tgacaccggc ctggcaggcc gccaggccgg
     7261 cgcgcatgcc ggcctcggtg gccttggcgg cgcgcttcat cacgtcgatc tcgagcggcg
     7321 acttggtgat gcgcccctcc tcgacgatgc cgtagcagtc ctgcagccgg ccgtctcgga
     7381 aggcgtcggc catgcggtcc tgctggtagg cggggaagaa gtagctgttg cgctcgtagc
     7441 cgatggcctt gccctcgagg ccgaactccc gcatcgactc ggtgagcagc tggatggcgt
     7501 cgccggtgtc ggtgtaggtg cgggtgagct cgacccacgt gcgctcgatg acgttggact
     7561 cctccaaggc ccgggtgatc gtgaacggct caccgtcgag ggggaccacg agcgcctgga
     7621 agaaggagta accggtggtc tggtagtcgg tgaggtacat gaggttgccc ggggcggtga
     7681 tgaccaccgc gtcgagcagg cgctcctcca tccgctcccg cagctcgcgg atgcgccgct
     7741 cgtactcctc gaacgggaag agcatgtcgt cgcgagccct catgtggcgc tccacgtggc
     7801 gtcgatggcg gcctccagga tgtcgaggcc ctcggtgagc tcgtcgtcgg ggatggtgag
     7861 cggcgggatc agcttgatga cccggccgcc cgtgccgcac ggcccgatga gcagcccgcg
     7921 ctcgaagcac tcgcgctgca cgtccttggc gtaggcgccg tcgacgacgt cgacggcctg
     7981 catcatcccg cgaccccgga cgtcccaccc gtggtcggct cgtgcgtcgg ccagcgagcg
     8041 cagccgctgg cgcatgacct cgcccttctc ggcgacctgg ttcatcaggg tgtcgtcggt
     8101 gaagtagccg agcgcctcgg tgccggcgac gaaggacagg ccctgcccgc ggaaggtgcc
     8161 ggtgtgctcg cccggcgacc agtgctggtc gtgctcgggc ttgttgaggt tcatggccag
     8221 cggggtgccg aagccgccga tgcccttggc cagggtgacg atgtcggggt cgaggtccat
     8281 gccgtcgaag ctgaagtacg aaccggtgcg accgcagccg gcctggatgt cgtcgatgat
     8341 gaacagcgcc cccacgtcgc gagcgaggtc ctggacggcg tgcagccact cgggcgaggc
     8401 cacgttcacg ccgccctcgg cctggatcac ctcgacgacg aacgccgcag gcggcagcag
     8461 gccgctcgag ctgtcctcga gggccgcccg gtagctctcc aaccccttca gcccaccgcc
     8521 cgcagcggtc tcgaagggca ggcgcaccac gtgctcgagg ggcacgccgg ccgcctggcg
     8581 gaagacgtcg ttggccgtga gggcgaggga gcccagcgtc atcccgtgga agccgtggct
     8641 gaaccccacc acgtggggcc gacccgtcac cttgcgagcc agcttcagcg cggcttcgac
     8701 cgcgttggtt ccggtcggac ccatgaactg catcttgtgg tccatgccgc ggggctccag
     8761 cacgacctcg gcgaagcgtt cgatgaagtc gcgcttgacg tcggtgtggg tgtcgaggct
     8821 gtgggcgacg ccgtcgcgct cgaggaactc gatcatcgcc cgcttcatgc gctcgtcgtt
     8881 gtggccgaag ttcaagacgc ccgcgccggc gaagaagtcg aggtagctgc gaccgtcctc
     8941 gtcgtgcatg cgggcgttct tcgcggaggt gaacaccgtg gggtaggcgc ggcagtagcc
     9001 gcggatggcc gactcccact gctcgaaggc ctcgttgttc acatcgtgct cctgtctctc
     9061 aggtcggttg gggaagcggg gccccggcga ggcgcgcgta gacgcgcacc acggggccga
     9121 tcagtcggtc gttgaagtcg tagtgcggac tgtggcacga ggcgtcgtgg ccctggccgt
     9181 cgtcggcgcc gaccagggcg aaggcccccg gcacctcggt gaggtagtag ctgaagtcct
     9241 ccgacgccat gatcggcacg gg
//
LOCUS       DATISU010000118         5830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_424979, whole genome shotgun sequence.
ACCESSION   DATISU010000118 DATISU010000000
VERSION     DATISU010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424979"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1960
                     /locus_tag="VLR27_04860"
     CDS             32..1960
                     /locus_tag="VLR27_04860"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HSP02807.1"
                     /translation="MSQLSDQVGRVVGGRYRLIAPLGAGSSAQVFLADDVTLQRKVAV
                     KMLQPALATDQHFLERFRAEAQTVASVNHPNVVVVHDSGVDDIPYLVTEYLAGGSLRG
                     MLAAGHRLTPSQALVVGLEACRGLAHAHGRGLVHRDLKPANLCFGDDGRLRIADFGLA
                     RALAESGTTDHGGNHLVGTMRYAAPEQARGERTGPKADVYALGLVLIEAVTGRVPFAG
                     GDPLAALQARTHGDLEVPDALGPLRPAIERAGRLDPDERADAEELGIALLAASERLTA
                     PAALPLVGALVEAAGTDESADERTQIAPAPTADVDDPATGRRGRKARKARKAAEAETV
                     ALADPDEPAARTRRRRWPVVLAVLLAVAITAGGLFLAQELRTPTAEVPELAGRQIDDA
                     ELIAAQNDWIVDPKKTRRDGTEPGEIVSTTPPPGDRLAEGGTLVVLVSEGNTLTDRPA
                     ELVGRPLEEVVLELEGVGLRHEVAEEVHHEEVPAGHVIGTVGDVEASLPKGSVVPLSV
                     SLGPAPRTVPELPDGAGFEVAREALAGVQLEASRADVFSETVPKGQVVALDPAPGATL
                     ARGETVTVQVSKGPDLVAVPDLRGMTLDEAEAALQAADLVLGQDCCASRGRVVGSDPG
                     AGTQVRRGTSVNIFLARG"
     gene            2043..2918
                     /locus_tag="VLR27_04865"
     CDS             2043..2918
                     /locus_tag="VLR27_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSP02808.1"
                     /translation="MASLDGRITVITGAGRGLGRAHALLFAAEGAKVVVNDLGSDREG
                     AGGDPSAAESVVEEIRAAGGEAVANHDDVASWAGGQALIQAAIDSFGGLDVLVNNAGI
                     LRDRALVNMTEEEWDSVTTVHLKGHFVPTRFAAAYWRDQSKAGTPRTASVIHTSSTSG
                     LLGNPGQTNYGTAKAGVAAFSQICAQELSRYGVRSNCIVPAARTRLTETAPGLSDIVA
                     APGDGGQFDFWDPAHCSPVVAYLAGADCPLNGRTMFVQGGTIRLMDGWQFGATFEQEA
                     RWTVAELHETLDRMA"
     gene            3152..4693
                     /locus_tag="VLR27_04870"
     CDS             3152..4693
                     /locus_tag="VLR27_04870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP02809.1"
                     /translation="MEGGRREPGGAGAHLDELSIDAPGADEVAVMPWSLLLRQRVHDR
                     ARRSSKYPWLVLTTVLLGLFSVGFTITILSVSIPRIAGDLGSDESTLTWLVTGPILAF
                     AIFGPSAGKLGDLRGHRRVYAWSMVAVIVFAALTAVAWDAPSLLAFRILGAATGAAVG
                     PTSLALINRLFPKERRAQAMGYWTMVGAGGPVVGVVAGGPIVEAFGWRWIFAGQVPLT
                     IAALALALAVLPETDKIPSARFDLAGAGTLAAAATAFLLAVNRGPVLGWTSPMVLGAF
                     ALTPVVLWLFVRVESRAVAPLLPLEYLRRRNFSIPILTQVFTNFAYMGGFIITPLFLQ
                     DIFGYGEAKTGSLLIARPLAFAVAGPVMGYLAARTGERFAAVGGAVAVLASMFGMASL
                     GAGSSDLLVVGFLGLSGIGLGMSAPALVAAIANAVDDDDLGVVGATQQMMTQFGVVVG
                     IQVMQSTQAARAGAVGAEASYGEAYLVGAAAAALGLAFAFFVRSSVVRTKAEEPVPER
                     QLQPA"
     gene            complement(4690..5097)
                     /locus_tag="VLR27_04875"
     CDS             complement(4690..5097)
                     /locus_tag="VLR27_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HSP02810.1"
                     /translation="MSKHEFLSEEWIAAAQAIREDYAGKSTTSPPPMKMNVVITEVPF
                     GEGSMDAHIDTTDGEMTLDMGHLDTSDLTVTVDYSTAKAIFIDQDQQASMQAFMAGKI
                     KVQGDMTKMMAMQTVQPDPAQVEMASKIAAITA"
     gene            complement(5115..5783)
                     /locus_tag="VLR27_04880"
     CDS             complement(5115..5783)
                     /locus_tag="VLR27_04880"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HSP02811.1"
                     /translation="MPDSTAAARPGTPSPADPDRQLTTQGRERKQQLVDCAAQLFTER
                     GFAETRIKDIVEAAGVAKGLFYWYFENKEALFAEVAADIRLRLRKHQASAIDPEADPL
                     RQIRQGAQASVQFMAENAHFFSLLEVETGAVTEESRRKGTAQHISDVRSIILRGQEAG
                     VIVDEEPDLLALAVVGTVGYYSHYHRTGRTNVGIEDLSRFVARSVVKVLAADDAAAAA
                     ALAE"
BASE COUNT          724 a         2227 c         2029 g          850 t
ORIGIN      
        1 cggggcggtt ccctactgtg gcgcagtggc catgtcacag ctctccgacc aggtcggtcg
       61 cgtggtgggc ggccggtacc gactgatcgc cccgctcggg gccggttcgt ccgcgcaggt
      121 gttcctggcc gacgacgtca ccctgcagcg caaggtggcg gtcaagatgc tgcaaccggc
      181 gctggccacc gaccagcact tcctggagcg cttccgggcc gaggcccaga ccgtggcctc
      241 ggtgaaccac ccgaacgtgg tcgtggtcca cgacagcggc gtcgacgaca tcccgtacct
      301 ggtcaccgag tacctggccg gcggcagcct gcgcggcatg ctcgccgccg gacaccgcct
      361 caccccctcc caggccctcg tcgtcggcct cgaggcgtgc cggggcctgg cccacgccca
      421 cggccggggc ctcgtgcacc gcgacctgaa gccggccaac ctctgcttcg gcgacgacgg
      481 ccggctgcgc atcgccgact tcgggttggc ccgcgccctg gccgagagcg gcaccaccga
      541 ccacggcggc aaccacctgg tcggcaccat gcgctacgcc gcgcccgagc aggcccgggg
      601 cgagcgcacc ggacccaagg ccgacgtcta cgccctcggg ctcgtgctca tcgaggccgt
      661 caccgggcgg gtgcccttcg ccggcggcga tcccctcgcc gccctgcagg cccgcaccca
      721 cggcgacctc gaggtccccg acgccctcgg acccctgcgc cccgccatcg agcgggccgg
      781 ccgcctcgac cccgacgagc gcgccgacgc cgaggagctc ggcatcgccc tgctcgccgc
      841 gtccgagcgc ctcaccgcgc cggcggccct gcccctcgtg ggcgccctcg tcgaggccgc
      901 tggcaccgac gagtcggccg acgagcgcac ccagatcgcc ccggcgccca ccgcggacgt
      961 cgacgacccc gccaccggcc ggcgcggtcg caaggcgcgc aaggcccgca aggcggccga
     1021 agccgagacc gtcgccctgg ccgacccgga cgagcccgcc gcccgcaccc gccgccggcg
     1081 gtggccggtc gtgctcgccg tgctgctggc ggtggcgatc accgccggcg ggctgttcct
     1141 cgcccaggag ctgcgcaccc cgaccgccga ggtgcccgag ctcgccggcc gccagatcga
     1201 cgacgccgag ctgatcgccg cccagaacga ctggatcgtc gacccgaaga agacccgccg
     1261 cgacggcacc gagcccggcg agatcgtctc gaccaccccg cctccgggcg accggctcgc
     1321 cgagggcggc acgctggtcg tgctcgtctc ggagggcaac acgctcaccg accggcccgc
     1381 cgagctcgtc gggcgacccc tcgaggaggt cgtcctggag ctggagggcg tcggcctcag
     1441 gcacgaggtc gccgaggagg tccaccacga ggaggtgccg gccggccacg tcatcggcac
     1501 ggtcggcgac gtcgaggcgt cgctgcccaa gggctccgtc gtgccgctgt cggtgtcgct
     1561 cggtccggcg ccgcgcaccg tgcccgagct cccggatggg gccgggttcg aggtcgcccg
     1621 cgaggccctg gccggcgtcc agctcgaggc cagccgggcc gacgtgttca gcgagaccgt
     1681 gccgaagggc caggtcgtgg ccctcgaccc cgcacccggt gccaccctcg cccgtggcga
     1741 gacggtgacc gtgcaggtca gcaagggccc cgatctcgtg gcggtgcccg acctgcgggg
     1801 catgaccctc gacgaggccg aggccgccct gcaggccgcc gacctggtgc tcggccagga
     1861 ctgctgcgcc agccgtggac gggtggtcgg ctccgatccc ggggccggca cgcaggtgcg
     1921 ccggggcacc tcggtgaaca tcttcctcgc ccgcggctga cccgccgact tgacctgtct
     1981 cctccgggga cagcactgtc tgctcgcgct cggcgggacg gcgccggcag aagggacacg
     2041 aagtggcttc gctcgacggc aggatcacgg tcatcaccgg agcgggacgc ggtctggggc
     2101 gggcgcacgc cctgctcttc gccgccgagg gcgccaaggt cgtggtcaac gacctcggca
     2161 gcgaccgcga gggcgccggc ggtgacccga gcgccgccga gtccgtggtc gaggagatcc
     2221 gggccgccgg cggcgaggcc gtggccaacc acgacgacgt cgccagctgg gccgggggcc
     2281 aggcgctcat ccaggccgcc atcgactcct tcggcggcct cgacgtgctg gtcaacaacg
     2341 ccggcatcct gcgcgaccgg gccctggtca acatgaccga ggaggagtgg gactcggtca
     2401 cgaccgtgca cctgaagggc cacttcgtgc ccacccgctt cgccgcggcg tactggcgcg
     2461 accagtccaa ggcgggaacg ccccggacgg cctcggtgat ccacacctcc tcgacgtcgg
     2521 gtctgctcgg caaccccggc cagacgaact acggcaccgc caaggccggc gtggccgcgt
     2581 tctcccagat ctgcgcccag gagctgtcgc ggtacggcgt gcgctccaac tgcatcgtcc
     2641 cggccgcacg gacccgcctc accgagaccg cccccggctt gtccgacatc gtcgccgccc
     2701 ccggcgacgg cgggcagttc gacttctggg acccggccca ctgctcgccg gtcgtcgcct
     2761 acctggccgg cgccgactgc ccgctcaacg gtcggaccat gttcgtccag ggcgggacca
     2821 tccgcttgat ggacggctgg cagttcggcg ccaccttcga gcaggaggcg cgctggaccg
     2881 tcgccgagct ccacgagacc ctggatcgca tggcctgacg ccgcctggct gcggcaccga
     2941 cgggtgccag tgccggacca gtgccagatg actaccatcg ccgtcacggg ccggtcccgg
     3001 tgggacggcc atgccggacg cagcgatccc cagccgctgc gacgaggcag gccgtccctc
     3061 cagggtcggc ccgtgccgcg ccccgcgtgc gtgaccgacg tctcaccgat caccttcttg
     3121 acccactggt caagtcgata gcgtcgatca ggtggaaggc ggacggcgcg aaccaggtgg
     3181 cgccggcgcg cacctcgacg agctgagcat cgacgcgccc ggcgccgacg aggtcgccgt
     3241 catgccgtgg tcgctcctgc tgcgccagcg ggtccacgac cgggctcgac ggtcgtcgaa
     3301 gtacccctgg ctcgtcctca ccaccgtgct gctcggcctg ttcagcgtcg gcttcaccat
     3361 cacgatcctg tcggtgtcga tcccgcgcat cgccggcgac ctgggatccg acgagtcgac
     3421 cctgacctgg ctggtcaccg gcccgatcct cgccttcgcc atcttcggcc ccagcgccgg
     3481 caagctcggc gacctccggg ggcaccgccg cgtctacgcc tggtcgatgg tggcggtcat
     3541 cgtcttcgcc gccctcaccg ccgtggcctg ggacgccccg tcgctgctcg cgttccgcat
     3601 cctcggtgcc gccaccggcg ccgcggtcgg gccgacctcg ctcgccctca tcaaccggct
     3661 gttccccaag gagcgccgcg cccaggcgat gggctactgg accatggtcg gcgccggcgg
     3721 gcccgtggtc ggcgtcgtgg ccggcggtcc catcgtggag gccttcggct ggcgctggat
     3781 cttcgccggc caggtgcccc tcaccatcgc cgccctcgcc ctcgccctcg ccgtcctgcc
     3841 cgagaccgac aagatcccct cggcccgctt cgacctcgcc ggcgccggca ccctcgccgc
     3901 cgcggccacc gccttcctcc tcgccgtcaa ccgcggtccg gtgctcggct ggacgtcgcc
     3961 gatggtgctc ggcgcgttcg ccctcacgcc ggtggtgctg tggctcttcg tccgggtcga
     4021 gtcacgggcc gtggccccgc tgctgccgct cgagtacctg cggcgccgca acttctcgat
     4081 cccgatcctg acccaggtct tcacgaactt cgcctacatg ggcggcttca tcatcacgcc
     4141 gctgttcctg caggacatct tcggctacgg cgaggccaag accggctcgc tgctcatcgc
     4201 ccggccgctc gccttcgccg tcgccggccc ggtcatgggc tacctggcgg cccgcaccgg
     4261 tgagcgcttc gccgccgtcg gcggtgccgt ggccgtgctc gcctccatgt tcggcatggc
     4321 ctcgctcggc gccggatcgt cggacctgct cgtcgtcggc ttcctcggcc tgtccggcat
     4381 cggcctcggc atgtccgcgc ccgccctggt ggccgccatc gccaacgccg tcgacgacga
     4441 cgacctcggg gtggtcggtg ccacccagca gatgatgacc cagttcggcg tggtcgtcgg
     4501 gatccaggtc atgcagtcga cccaggccgc ccgggccggc gcggtcgggg ccgaggcgtc
     4561 ctacggcgag gcctacctgg tcggcgccgc cgcagccgcg ctgggcctgg ccttcgcctt
     4621 cttcgtgcgc agctcggtcg tgcgcaccaa ggcagaggag ccggtgcccg agcgccagct
     4681 ccaacccgcc taggccgtta tcgccgcgat cttgctcgcc atctcgacct gggccgggtc
     4741 gggctggacc gtctgcatcg ccatcatctt ggtcatgtcg ccctggacct tgatcttgcc
     4801 ggccatgaag gcctgcatgc tggcctgctg gtcctggtcg atgaagatcg ccttggcggt
     4861 ggagtagtcg acggtgacgg tgaggtcgga ggtgtcgagg tggcccatgt cgagggtcat
     4921 ctcgccgtcg gtggtgtcga tgtgggcgtc catggagccc tcaccgaagg gcacctcggt
     4981 gatgacgacg ttcatcttca tggggggcgg ggaggtggtg gacttcccgg cgtagtcctc
     5041 gcggatggcc tgggcggcgg cgatccactc ctcggacagg aactcgtgct tcgacatggc
     5101 cggaaatgta tcggtcactc ggcgagggca gcggcggccg cggcgtcgtc ggccgccagc
     5161 accttcacga ccgatcgggc cacgaaccgg gacaggtcct cgatccccac gttggtgcgg
     5221 ccggtgcggt ggtagtggct gtagtagccg acggtgccga ccacggcgag ggcgagcaga
     5281 tcgggctcct cgtcgacgat gacgccggcc tcctgtcccc gcaggatgat cgagcgcacg
     5341 tcgctgatgt gctgggccgt gcccttccgg cgggactcct cggtcaccgc gccggtctcg
     5401 acctcgagga gggagaagaa gtgggcgttc tcggccatga actgcacgct ggcctgggcg
     5461 ccctggcgga tctggcgcag cgggtcggcc tcggggtcga tggccgaggc ctggtgcttg
     5521 cggagccgga ggcggatgtc ggcggcgacc tcggcgaaga gggcctcttt gttctcgaag
     5581 taccagtaga agaggccctt ggccacgccg gcggcctcga cgatgtcctt gatacgcgtc
     5641 tcggcgaagc cccgctcggt gaacagctgc gccgcgcagt ccaccagctg ctgcttgcgc
     5701 tcgcgcccct gggtcgtcag ctggcggtcc gggtcggcgg gcgagggggt gcccgggcgc
     5761 gcagcggcgg tcgagtcggg cacccgcaga gtgttccgcc accttgacca ccaggtcaag
     5821 caccgcttcc
//
LOCUS       DATISU010000119        29937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_704361, whole genome shotgun sequence.
ACCESSION   DATISU010000119 DATISU010000000
VERSION     DATISU010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 29937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29937
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704361"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VLR27_04885"
     CDS             <1..501
                     /locus_tag="VLR27_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02812.1"
                     /translation="RANADLEPYGKPLYAGLAALERFDDPLADSWRLADRLREYRGDA
                     HTAAWTSVGLDACQIGVMSELYWGLPARSYSRTRMWSDEQYDAAEAALEGRGLLADHQ
                     LTEQGRAFREEIEVVTDRGCRPIVEALGDDVDALTTILGRWGAAVRDARGYPASGPHD
                     LADAAT"
     gene            552..1202
                     /locus_tag="VLR27_04890"
     CDS             552..1202
                     /locus_tag="VLR27_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HSP02813.1"
                     /translation="MPRLRQVDRAEAMAGDGHGIVAAMYRLIFGDRDPVAEPGTATGT
                     PGDWWTVWAGSPDILQHAVQGFGLYQSPDRQLDPVLRELGQARAGYSAGSRFVFSQHC
                     KSLRGLGVSEERIGAVAHWPVADCFDEVERLVLAYTDCLVLQHGRVPDALFDALRRHL
                     SDVELLELTYITTLYFQHAVMSRALRTELDDVDEPVVEVRVPGAEDTIVPLTPKER"
     gene            complement(1210..1860)
                     /locus_tag="VLR27_04895"
     CDS             complement(1210..1860)
                     /locus_tag="VLR27_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP02814.1"
                     /translation="MSNEATTRTPSRWNGLSTEDRQRERRNLLIEAAFELLATEGMSG
                     TTVRAVCAQARLNPRYFYESFTDLDELLVAVYDDVFRQLQRRSAAAVAAAEDDAAAMR
                     ASVAATVHFIAEDPRRGRILYSEALGNEALNLRRVRTGATLVELVFQDNTRRDVGAQD
                     ERIGRLGASVLVGGFSELLVAWIGGRIDLTAEELIDDATEVFLAVWDATRRLHAAH"
     gene            complement(1907..2353)
                     /locus_tag="VLR27_04900"
     CDS             complement(1907..2353)
                     /locus_tag="VLR27_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HSP02815.1"
                     /translation="MDAIDRKIISLLVEDGRLSVTELADAVHLSLSAASQRLRRLRES
                     GAIDRFTIRLDSEAVGRPIDALVDLQLTPGAPWSGIDDALRHNPEVVDAVHVTGGFDY
                     QIRVRTRTIADLDQLLQNFKEHLGVRETSTRVVLHTVEGFPREPAV"
     gene            2437..3018
                     /locus_tag="VLR27_04905"
     CDS             2437..3018
                     /locus_tag="VLR27_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HSP02816.1"
                     /translation="MILLVGFVTGLALVAPIGPVALTLFGLGAERGRRVALAGAGGVV
                     LADALTMPVALGSAGFLSSLDSGIVRGFEVVMGLALVALAVSTIVATDRARAAVAGIR
                     RPTRTLAFMTIVNPLSLVAWAGVALALPSSLRSPAALVAFGIGLVLASAFWHSGLAMA
                     AGSLAQRMGDGPRRAVTRASGVVLLVVGGVLVA"
     gene            complement(3092..6406)
                     /locus_tag="VLR27_04910"
     CDS             complement(3092..6406)
                     /locus_tag="VLR27_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase"
                     /protein_id="HSP02817.1"
                     /translation="MATLDEPVDHEAERADAALLADASVDVVLRWLDAAAAAETGEDR
                     RSARRLHRLVDDPDGVAFTMRFVDRVIRPDDHHVAAHQLHALAGGRSSLPRFLSPVDR
                     VLLRAGANLAPVLPRLVMPLARRRMRQIVGHLVVDAHGAAMDRHLGARQAEGFDLNVN
                     LLGEAVLGEREAARRLQATIDLLGDEHVDYVSVKISGVASQLQHWAWDDSLDRIAERL
                     RTILRAARARGTFVNLDMEEYPDLELTMAAFRRVLDEDEFLGTEAGIVLQAYLPDAFD
                     ALGRLVAWAGERQDRGGADIKIRLVKGANLAMERVDAAMHGWDQAPHPTKADTDAGYK
                     RCLDWVLEPERLRAVRIGVGSHNLFDVAWAALLAQRRGVADRVGIEMLEGMAPAQARV
                     IRDELDSLLLYTPVVDPDDFDVAISYLFRRLEENAAVGNFMRALGDLEPGSASFVEQA
                     AAFRRAVAQRWDVSTTPRRTVDRSQPPEPVGLAPFDNEPESDPAVAGTRAWALEHLAR
                     TPEAVRAPLTTETSAIDEMIERARRAQGEWWARPAEERRAVLRAVGDEMARRRGDLLT
                     VMAHEGRKTVGQADPEICEAIDFARYYADRSDLADPTGTTRDHARFEPLGVVAVVPPW
                     NFPVAIPAGGMLAALAAGNAVLAKPAPEVPRCSELVVEIAHAAGVPVDLLQYVRVPED
                     DVGRHLLTSVDAVILTGGTDTAELFRSWDPGLRLFAETSGKNAMVITPNADLDLAVAD
                     LVASAFGHAGQKCSAASLAICVGEVYDSPRFRRQLVDAVESLDVGPGHHPTTTMGPLI
                     GQPNPRLARAFATLDPGEEWLVEPRPLDDSGELWSPGVRIGVRRGSWFHQTECFGPVL
                     GLIRAEDLDEAIAIQNDSAFGLTGGLHSLDPAEVAAWTEQVEVGNAYVNRGITGAIVQ
                     RQPFGGWKASSVGPGAKAGGPGYVVQLGTWHPTGPLDRDVWTRSDAAWWRTVYGIDHD
                     PTGLFCESNILRYRPLPRVGLRIGADADDVAVERVRDAAETCGVPLVESSHDDESDDA
                     FAARLPDLDVERVRIVGATSDVVRQAAIEARVHLDDDPVTADGGVELRRYLREQAVTR
                     TLHRFGNLVQADTAHP"
     gene            complement(6443..6910)
                     /locus_tag="VLR27_04915"
     CDS             complement(6443..6910)
                     /locus_tag="VLR27_04915"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HSP02818.1"
                     /translation="MQTPATAALDAAAVDYRTTEYEPPAERGYGEGLVTVLDLDPDTV
                     GKTLVAVLDEARHVLAVVPVSATLDLKALARLAGAKRAAMADPAEAERLTGSVVGGIS
                     PLGSRRPLPVFVDELLAVADEVHVSGGRRGLELSLAPADLVRVAGAVVGPIAT"
     gene            complement(6918..7079)
                     /locus_tag="VLR27_04920"
     CDS             complement(6918..7079)
                     /locus_tag="VLR27_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02819.1"
                     /translation="MTETAQCPFCELRFSARWELKLHLDAEHEGRVRDKDDAHVAIEE
                     NDPDNPKPL"
     gene            complement(7121..7684)
                     /locus_tag="VLR27_04925"
     CDS             complement(7121..7684)
                     /locus_tag="VLR27_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02820.1"
                     /translation="MASIDVAGFVADLKDHAVDHGFHVHDERHFVETYSLRQAWEVDL
                     HPEEACGGPLDLHLALDVDPRSLLAFEDVVLAIGEDDDPPDEFHFPLIFTWSLPPLPK
                     GPDLLVLATDLAGLGGPDLPLEVSAIDSFGAVTDAPERSLAIVAKVDVSLARVFLGQE
                     QLCDTLDRCLAVSHFLLDRAPVWLDEV"
     gene            7754..8557
                     /locus_tag="VLR27_04930"
     CDS             7754..8557
                     /locus_tag="VLR27_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSP02821.1"
                     /translation="MRDDDLLAVLREAAVAVREALDGLDDWGLAGTRSGQYRSDLAAD
                     DACLAVLDDAGFGWLSEESGVEHADREITVVVDPVDGSTNASRGIPWYATSLCAVDAG
                     GPRVALVVNQASGRRYEAVRGRGATVDGRPVAPSGETDLGRSVVGLSGYPNQYLGWKQ
                     YRALGAAALDLCAVADGVLDGYIDCSPSAHGSWDYAAGMLICREAGIEVVDAFDRELL
                     DPAALRHGDRRTPVAGATPALTAELVDARRRLRWPDDPDPAGLVATDRH"
     gene            8569..9090
                     /locus_tag="VLR27_04935"
     CDS             8569..9090
                     /locus_tag="VLR27_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSP02822.1"
                     /translation="MAGLLYRLALQVYRRLPAGLRRRVVRSISPSFTVGAMCFIERAD
                     GALLLVRHSYRERWGVPGGLLQKGEDAEVGARREVREEVGLVVELLGEPAVVVDADPQ
                     RVDVVFRARPAEGTDTEPDVADSAEIVEARWFAPDALPELQFETAGALVALARIATSA
                     GQRPLPEPVWPRF"
     gene            9276..10118
                     /locus_tag="VLR27_04940"
     CDS             9276..10118
                     /locus_tag="VLR27_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSP02823.1"
                     /translation="MERIVVGTDGSPGGADAMRWAATLARSQGAELVAMTSCAPVEAE
                     LPPARATELLDEQRAHLQEWIDAAHLGDVAVRTVVEPGDHRPGILRLALREEADLVVV
                     GRVGSSAGPGLLHLGSMAEWLAHRADCPVAVVGGAVELAPTRVVVGVDGSAGSQAAVD
                     WVRTLGQPTTLRVIALSVHEPLVEWTPANSPENWRRGLEQRIVTDMAAPLFDAEMEVE
                     ARACAGPNVADALLQVADDEGADLVVVGTRGLGGFSGLRVGGVALKVLHRADRPVVLV
                     PPGS"
     gene            10115..12838
                     /locus_tag="VLR27_04945"
     CDS             10115..12838
                     /locus_tag="VLR27_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HSP02824.1"
                     /translation="MTAAATPVDAHDRGATELAAAGAHAAEVDAVLEALGASDSGLTS
                     VEATRRLAEFGPNRLPDPPRAGVLVRVLRHFNDVLIYILLVAAGVTAVLEHWIDAGVI
                     LAVVVVNALIGYVQEGKAEHALEGIRKMLSLEAQVRRDGHWTRIGADELVPGDVVRLR
                     SGDRLPADVRLLEATNLQVEESALTGESLPSDKRTEPVAPDAGIGDRTGMAFSGTLVT
                     AGRGVGVVTATGPATEIGQINTMIGQVQTLATPLTRQMRAFGTQLSVAIVGLAVVVFV
                     LGFVLHDYPASELFLAAIGFSVAAIPEGLPAILTITLAIGVQRMAGRNAITRRLNAVE
                     TLGSVTVVCSDKTGTLTKNEMTARCVVTRAGHYEVSGTGYEPAGELSLHGEHTALADH
                     ADLLALVEAMAVANDTDIVEVDGRWTVNGEPTEGSLRALATKLGFDGSGYRRTAVVPF
                     ESEHKFMATVDEVPGGGHVLLVKGAPDRLLERARRELGADGRLAPLDVAWWESQVELL
                     SAEGLRVLAAARCDDGGPRDELSLEDVHGLVFVGIVGIVDPPRPEAIESIATCGRAGI
                     RVVMITGDHAGTARAIGREMGIGDGSAAVTGTELEAASDEELQAIVRTNDVFARTSPE
                     HKLRLVTALQANGEVVAMTGDGVNDAPALKRADVGVAMGIKGTEATKEAAEIVLADDN
                     FASIERAVEEGRTIYDNLRKAILFILPTNAAEALVIVVAVVVGLDLPLTPTQILWVNM
                     VTAVTLALALAFEPPEPGIMDRPPRPTGASILDRYFLWRIGFVGALVGGATIAVFLLS
                     DRSEDSLAVARTVAVNTLVFGQICYLFNSRFLREASYPPARLLANPVAWIAVAVLTAL
                     QLGFVYLPFMNTTFGTAPLDLVHWVVPGLIGMAVFGAVEIEKAIARRRVRP"
     gene            complement(12835..13785)
                     /locus_tag="VLR27_04950"
     CDS             complement(12835..13785)
                     /locus_tag="VLR27_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP02825.1"
                     /translation="MADPKRSGHRGARGPDDLVGRYLADIGQYPLLTAAEEIELAQAI
                     EDGREAELRLAEELDLKPAARASLRRQVRAAAAAKQRFIQSNLRLVVSIAKRYQSTGL
                     PLLDLIQEGNLGLIRAVEKFDHHKGFKFSTYATWWIRQAIGRGIADKGRTIRLPSHVV
                     DTLSTLSKASGTLLKELGREPTAAELSELTGMPVDRVETAMQATADVISLSAGIGEDA
                     TEFGDLLPDDKAEVPFDVAAANLEKVELRNLLLTLSDREREVLELRFGLVGERPLTLD
                     EVGRHFELTRERIRQIEAKALTKLRHPCSPAQRQGRAMAV"
     gene            13908..13981
                     /locus_tag="VLR27_04955"
     tRNA            13908..13981
                     /locus_tag="VLR27_04955"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13942..13944,aa:Val,seq:tac)
     gene            complement(13996..15303)
                     /locus_tag="VLR27_04960"
     CDS             complement(13996..15303)
                     /locus_tag="VLR27_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HSP02826.1"
                     /translation="MHHLFRRPLRVLLVFVLAVAVAAAGAPAGAQSDAKAQRDEVQRK
                     RAALAADIDVLRATDAQVEAALRALQGNVAARTADLRDAERRAASAQVALETATDQVE
                     SKQAEIAVLDQAAKDVVVAAYMQPTASAGILDALATSSIGEAELKQALLEARSSDQFD
                     VLDQLERARDDLEVARAEAESAAVEAREQQAAVGNQLEEVQQATSQQEAVVAEVQDRL
                     DHRLSEAQSLAELDRELAAQIEEEQRRLAAELQRQQQAREQAEAAAAARAAAATRTAS
                     ESRSSAPRPAPAAPAPAAPSPPRTVPITGSGSIVSVRGIRVHNTIASSLADMLGAADA
                     AGISLSGGGYRDPAGQIAVRRNNCGTSNYAIYEMPASSCRPPTARPGTSMHERGLAID
                     FTYGGRIISSRSSAAFQWLNANASRYGFYNLPSEPWHWSVNGR"
     gene            complement(15411..16565)
                     /locus_tag="VLR27_04965"
     CDS             complement(15411..16565)
                     /locus_tag="VLR27_04965"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HSP02827.1"
                     /translation="MSPTDDDASFETRCVHGAGEPDAVTGAVVPAISLSSTFAQDGVG
                     QQRGFEYSRSGNPTRAALERHLASLEGAAHGFAFASGLAAEDAVLRLLAPGDGLLLSD
                     DAYGGTFRLARQVHGPAGLVVRTTRLSDVDAVVREWSDGTRLLWAETPTNPWLRIADI
                     AALADVAHERGALLVVDNTFATPALQQPLDLGADIVVHSTTKYLGGHSDVVGGFVATN
                     DDEVAERIGFLQNAAGAVPGPLDCYLVHRGCRTLHLRMERHSANAEAVATMLGDHPAV
                     AEVLWPGLAAHPGHDVAARQMSAFGGMVSLRLRDGLDAALRLCESTQVFTLAESLGAV
                     ESLIEHPGRMTHSSVAGSENEVPDDLVRLSVGIEAVDDLVGDLQQALERC"
     gene            complement(16562..18223)
                     /locus_tag="VLR27_04970"
     CDS             complement(16562..18223)
                     /locus_tag="VLR27_04970"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HSP02828.1"
                     /translation="MDQLVAVGDVEVPARLQARVRRVVRHQPGADTAPLIADLRTHVP
                     RRVLVVGDGATARSLALDLHDHGLPSVVYTDDDVSLPQSVPHRWLTDPAGDMDVADDL
                     LALIGNTPMMRLDRTGRDLDCHLLGKLELFNPGFSSKDRPALAMIDAAERDGLLQPGG
                     TIVEPTSGNTGVGLAIVAARRGYHCVFVCPDKVATDKIAQLRAYGAEVVVCPTSVDPE
                     HPESYYSVSDRLAREIPNAWKPDQYHNPNNPQSQYDTAGPEIWAQTKGRITHFVCGVG
                     TGGTISGIGRYLKEQNPDIRVIGADPEGSVYSGGSGRPYLVEGIGEDFWPSTYDPDIA
                     DEIVAISDAESFAAARRVTRQEGLLIGGSGGTAIAAALRIGHDLPQDAVVVVHIPDSG
                     RGYLSKLYDDRWMADHGFLRSSGPTVEDLLTGRDPELPLLVHTHPDETVRTAIEIMRE
                     YGVSQLPVVKAEPPVVLGEVMGSVTEVGLLDHVLADASVLERPVQDVIGPAFPTLGLG
                     EPIDAAIATLEDASAALVLDAGHPVAVVSRADILRSMTSDQDQRR"
     gene            18304..18714
                     /locus_tag="VLR27_04975"
     CDS             18304..18714
                     /locus_tag="VLR27_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HSP02829.1"
                     /translation="MNEPTARTPAARVLRNGPRRLFPPDPRTEGSDPDYRFSLANERT
                     FLAWIRSSLALAAGGLGAVALLDDFPGEEYLGLALLALSFITAGLSYRRWALAETAMR
                     LGRPLPPSRLPLLLAIAVSAVAVASAVLVAVNST"
     gene            18711..19049
                     /locus_tag="VLR27_04980"
     CDS             18711..19049
                     /locus_tag="VLR27_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HSP02830.1"
                     /translation="MRPEQVFDPLLQQERTSLAWERTAFAGIVVGSLLTRVGASVHVL
                     LGGFGLLQVCASAGLLVWSGRHYEDLHVQLRAGETPTHPRAAMVVGVGATVATGLATA
                     LALLFVITDS"
     gene            19106..19462
                     /locus_tag="VLR27_04985"
     CDS             19106..19462
                     /locus_tag="VLR27_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02831.1"
                     /translation="MAFGQQSGPPATARQVQQLTELLHAAGHTDFRDARGPLGLTQRQ
                     AGGKFTRDEADELIARLEGEAEGAEAPPGEVPRTAAATDRYDRREAQRLAALRQMPDE
                     ALAAELQRRGWVVMEP"
     gene            complement(19473..20693)
                     /locus_tag="VLR27_04990"
     CDS             complement(19473..20693)
                     /locus_tag="VLR27_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HSP02832.1"
                     /translation="MPDALIIDAVRTPRGIGKQGKGALAHLHPQHLGRTVLEALVDRN
                     GFDTADVDDVVWGTSAQSGPQGGDIGRMSALDAGYDTKASGVTLDRFCGSGITANNIA
                     AHGIMAGMDDLVVAGGTEMMSIKHKGMLPMGAGNAHLQELHPQSHQGVCADAIATLEG
                     IDREALDAHAVESQRRAAAAIAEGRFDKSLVTVKNLDGSVALDHEEFPRPQTTMESLA
                     QLAPSFPAIADYSHEGNPTFRELINQKYPDLDIAHVHHAGNSSGVVDGAAAILWASPD
                     YAKAHGLTPRARVVATATVGDDPTLMLNAPVPAARKVLAKAGMTIDDIDLFEVNEAFA
                     VVSEKFQRDLDLDRDKVNVNGGAMALGHPIGATGAMLIGTVLDELERRDGQFGLITMC
                     TGGGMAPAIIIERV"
     gene            20933..22471
                     /locus_tag="VLR27_04995"
     CDS             20933..22471
                     /locus_tag="VLR27_04995"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSP02833.1"
                     /translation="MTRLGFLLDSDSCIGCHACTVACKSEHDVPLGVNRTWVKYIETG
                     TFPDVARKFSVMRCNQCDDAPCMKICPTSALYRDDKGVVDFDDSNCIGCKSCMNACPY
                     DALYINPETNTAHKCNMCNHRLEVGLEPSCQIVCPTQAITIGDLDDPTSKISRIVARD
                     DVAVRAPEQNTKPKVFYRGADQASLDPTRTAIANDGMIWAETTPSHPTVPVALGRKPT
                     DEDGVVGRTAYTTAHHMTWKSRVSGYLVTKAVAAGVMAVAALLVLLGHEDVQAAVGVV
                     PPVVAGVFLAATGFLLVTDLKQPTRFHYLLTRGNWSSWLVKGAYVLMAFAVVCGMWFL
                     AGVLDQTDVLPYLAPFAALGAAGTAGYTAYLFAQCEGRDLWQTPLLLPVLLAQAVTAG
                     GAVYAVLDVFMDVPAPTAIRWVLLGGVLATAALLAVELTSHGSRHVELAVHAMTRGTW
                     AKQFWWGGVVLGLVVPFVLTVAALAADIDAPALVALAGLAALGGMFAYEDAFVRAGKS
                     VPIS"
     gene            22485..25307
                     /locus_tag="VLR27_05000"
     CDS             22485..25307
                     /locus_tag="VLR27_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSP02834.1"
                     /translation="MTELHNYPPHEQWHDWRELDAKAWPEKLERKYTLVPTTCFNCEA
                     GCGLVAYFDQETGKIQKIEGNPEHPGSRGRNCAKGPATLNQVYDPERILHPMKRAGER
                     GEGKWERISWDQALDEIGARLRQAIVEDRHNEIMYHVGRPGEDGYTDRVLKAWGVDGH
                     NSHTNICSSNARIGYQSWMGHDRPSSDFANAEVIFLISAHLEAGHYFNPHAQRIMEAQ
                     AKGAKVICVDPRLSNTGAKADHWLAPWPGTEPYLLLAIARILIEEGTWHEEFVRRWTN
                     WETYLQRTRPDLPVEWESVRTALLDEYAEYTPEAAEQKTAIEAGTIRELAELIGAHPT
                     KFASHNWRAAGAGHLGGWQVARCLFFLNVLTGSVATKGGTAGNGMNKFKPAAPIGAPT
                     IPRWNELEWPTEYPLSYHEMSILLPHFLKEGRGSLEVYFSRVYNPIWTNPDGFTWMEA
                     LTDTDKVRCHVALTPTWSETAWFADYVLPMGVSVERHDVHSYETHAGRWIGFRQSAFR
                     RYAEIKNGGPLDPGTRSHEYNPGEVWEENELWIDLSWRIDPDGSLGIRKWFESEERPG
                     EPVTVDEYYERMFTDSVPGLAKAAAEVGQTPLEYMRDRGAYAVPTDPYEPYERPVDPA
                     DLDGCTKDGDGVYRKPGTAGAWSGDLDELDDLKLVPLGDGSPAVEVDGEAKEGFPTPS
                     KKLELFSTTLADWGWPEYATPKWIPSQVHWEDLDLAGTERILLPTFRIPTLIHTRSAN
                     SKWLNEISHRHPLWIHPEDAEKLGIEEGGLVRVSTRIGHFVITAWRTEGIRPGVVAAS
                     HHMGRWRLDEDKARSWGAGKARIEHDGSTWKLVREHGNQPYESSDPDTERIWWNDTGV
                     HQNATFCVQPDPISGMQCWHQKVDVTPAQSGDEYGDVVADTAKSREAYLEWKAMTRPA
                     TGDLRRPLWYARPLKPTAGAYRRPGA"
     gene            complement(25314..25568)
                     /locus_tag="VLR27_05005"
     CDS             complement(25314..25568)
                     /locus_tag="VLR27_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HSP02835.1"
                     /translation="MAKDHGSSIKDDEQYEALRDDGMSKEKAARIANSDRSRTGQKGG
                     SSPAYEDWTKDDLYDKAKDVGIEGRSDMSKDELIDALRNH"
     gene            25670..26332
                     /gene="phoU"
                     /locus_tag="VLR27_05010"
     CDS             25670..26332
                     /gene="phoU"
                     /locus_tag="VLR27_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HSP02836.1"
                     /translation="MTDQPGHVRVSFSAHLEEIERLVMRQFGMVIEGIAGATTVLLAD
                     DAEAAKRLIARDEMIDDLEYQIEQSVEQQLLLESPVARDFRFLMTVSRIVPELERSGD
                     LAEHVAAEAARGFGRALTPPLRGAVQDMGDIAGAMWRRCADAFRDHDGEAAVELDRTD
                     DELDAIHRRFVDEAAEQLTGEDVSSAALVGRYYERLGDHAVNIAGRVCYLATGRPQRK
                     TD"
     gene            complement(26329..26622)
                     /locus_tag="VLR27_05015"
     CDS             complement(26329..26622)
                     /locus_tag="VLR27_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HSP02837.1"
                     /translation="MIRLSVLYPKTEGAQFDHDYYRDKHVPLACETWGIEPSGAQIDK
                     GVDGPYEAAVHFSFDSLEAMGAAMGGEGTQAVIADVANYTSIQPVMQTSEIVG"
     gene            26713..27513
                     /locus_tag="VLR27_05020"
     CDS             26713..27513
                     /locus_tag="VLR27_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSP02838.1"
                     /translation="MTGVLDRLFGVEGKQVVVTGGSRGIGAMIAQGFVEAGAHVIISA
                     RKAEACDATAAELDALEGSGSCTSVPADLSTQEGVDALAAAVRERCGGSLHVLVNNAG
                     ATWGEPLDEYPESAFDKLFNINVKGAFLLTQALLPELRAAGTEEDPSRVINIGSIDGI
                     RTPAMETYAYSTTKAGILALTRHLAKNLVRDHVNVNAIAPGPFESKMMAFALGSDEGK
                     EMVASGVPMRRIGRPDDMAGAALFLAGRGGSYLCGETIVVDGGIANVG"
     gene            complement(27584..28234)
                     /gene="msrA"
                     /locus_tag="VLR27_05025"
     CDS             complement(27584..28234)
                     /gene="msrA"
                     /locus_tag="VLR27_05025"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HSP02839.1"
                     /translation="MPLFGRDKTTMVSPEDAIPGRDQPIPVSDTHVVLGTPMEGPWPD
                     GHEVLYVAMGCFWGAERIYWQIEGVHSTAAGYMGGWTKHPTYEETCTAMTGHTESVQV
                     VYDPSVVDIDVLLQAFWENHDPTTPNRQGNDVGTQYRSAIYTTTDAQHEAVLASRDKY
                     QAALHEQGFGDISTEIARSSDAGDGVFYYAEDYHQQYLHKNPRGYCNHGFCQVAYS"
     gene            28302..29024
                     /locus_tag="VLR27_05030"
     CDS             28302..29024
                     /locus_tag="VLR27_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HSP02840.1"
                     /translation="MLEDHEVERILVVTAHPDDVDFGVAGSVARWTAAGAHVTYCILT
                     DGDAGGFDPDVPRGDIAGIRRGEQTNAATQVGVEDLHFLGYPDGRLEVSLDLRRDISR
                     IIREVRPRRVVTQSPLRTMDSVYRNHPDHLACGEATCCAVYPDSRNPFTFTELLAEGL
                     EPWTVEELWVMGTVPGAQEAFVDITDTFDRKIKALLCHVSQMTDPDGIEDRLREWNGA
                     NAEAGGLPAGTLAERYVVMPTV"
     gene            complement(29041..29856)
                     /locus_tag="VLR27_05035"
     CDS             complement(29041..29856)
                     /locus_tag="VLR27_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02841.1"
                     /translation="MSLVTLDDGILAWLAEDPGDGRPNAGLVVDADAATAIDTLCVPS
                     QTAAFVAAIDALGLRVRRTVLTASTIEFVGGSALFTMSGMYGRRQTSVHLDQPPTPDI
                     YRRLLPDVAGEFDDELTTKPVSHIVDERVQLTPAVSLHPCSGAQEENLVAVVDAAGIC
                     FAGAMASFGVTPRCYQGDPARWADELDRILELAPIIVPGHGRIGGEEEVRELQGYLRA
                     CVAAAGDPAAISAGPWDEWRQREHDEINVERAAMLARGDTSIPPSMLRAAGLA"
BASE COUNT         4017 a        10550 c        11050 g         4320 t
ORIGIN      
        1 cgggcgaacg ccgacctcga gccctacggc aagcccctgt acgccgggct cgccgcgctc
       61 gagcgcttcg acgacccgct cgccgacagc tggcgcctcg ccgaccggct gcgcgagtac
      121 cggggcgacg cccacaccgc ggcatggacc tccgtcgggc tcgacgcctg ccagatcggt
      181 gtgatgagcg agctgtactg gggtctgccg gcccggtcgt actcgcgcac ccggatgtgg
      241 agcgacgagc agtacgacgc ggccgaggct gcgctcgagg ggcgtgggct gctcgccgac
      301 caccagctga ccgagcaggg acgggccttc cgcgaggaga tcgaggtcgt caccgaccgg
      361 ggttgccgcc ccatcgtcga ggcgctcggc gacgacgtcg acgcgctcac cacgatcctc
      421 gggcggtggg gtgccgcggt gcgcgacgct cgcggctacc cggcctccgg tccccacgac
      481 ctggccgacg cagccacctg agcccgggtg ctgcggcgga cgcactgccg gttccgacga
      541 gggagatgcc catgccccgc ctgcgccagg tggaccgcgc cgaggccatg gccggagacg
      601 gccacggcat cgtcgcggcg atgtaccggc tgatcttcgg cgaccgggac ccggtcgccg
      661 aacccgggac cgccaccggc accccggggg actggtggac cgtgtgggcc ggttcgccgg
      721 acatcctcca gcacgccgtc cagggcttcg gcctctacca gagccccgac cggcagctcg
      781 accccgtgct gcgagagctc ggacaggcgc gagccggcta cagcgccggc agccgcttcg
      841 tgttctccca gcactgcaag tccctgcgcg gcctcggcgt gtccgaggag cgcatcgggg
      901 cggtggccca ctggcccgtc gcagactgct tcgacgaggt cgagcgactg gtgctcgcct
      961 acaccgactg cctcgtcctc cagcacgggc gggtgccgga cgcgctcttc gacgcgctgc
     1021 gccggcacct gtcggacgtc gagctcctgg agctgaccta catcaccacg ctgtacttcc
     1081 agcacgccgt gatgtcgcgc gcgctgcgca ccgagctgga cgacgtggac gagcccgtcg
     1141 tcgaggtccg cgtgcccggc gccgaggaca cgatcgtgcc cctgacgccg aaggagcgtt
     1201 gaccggacct cagtgggcgg cgtgcaggcg gcgggtggcg tcccacacgg cgaggaagac
     1261 ctcggtggcg tcgtcgatga gctcctcggc ggtgaggtcg atccgcccgc cgatccaggc
     1321 gaccagcagc tcggagaagc cgccgacgag cacggaggcg ccgagccgac cgatccgctc
     1381 gtcctgggcg ccgacgtcgc gtcgggtgtt gtcctggaag acgagctcga cgagggtggc
     1441 gccggtgcgc acccggcgca ggttcagggc ctcgttgccc agcgcctcgc tgtagaggat
     1501 gcgaccacga cgggggtcct cggcgatgaa gtgcacggtg gcggcgaccg acgcccgcat
     1561 ggcggcggca tcgtcctcgg cggcagccac cgccgcggcc gagcgccgct gcagctgccg
     1621 gaagacgtcg tcgtagacgg caacgagcag ctcgtcgagg tcggtgaagc tctcgtagaa
     1681 gtagcggggg ttcaggcggg cctgcgcgca gacggcgcgc acggtcgtgc cggacatgcc
     1741 ctcggtcgcc agcagctcga acgcggcctc gatgaggagg ttccgccgct cgcgctggcg
     1801 atcctcggtc gacaggccgt tccagcgcga cggcgtgcgg gtggtcgctt cgttcgacac
     1861 accggtgatg gtactgctgg tcacgcccgt tgccagttga tcgtgctcag acggccggtt
     1921 cgcgggggaa cccctccacc gtgtgcagga ccacgcgcgt cgaggtctcc cgcacgccca
     1981 ggtgctcctt gaagttctgc agcagctggt cgaggtcggc gatggtccgg gtccggacgc
     2041 ggatctggta gtcgaagcca ccggtcacgt ggaccgcgtc gacgacctcg gggttgtgcc
     2101 ggagggcgtc gtcgatgccg gaccacgggg cccccggcgt cagctgcaga tcgacgagcg
     2161 catcgatcgg acgcccgacc gcctcgctgt cgagccggat ggtgaaccgg tcgatggccc
     2221 cggactcccg gagacggcgg aggcgctgcg aggccgccga caggctcagg tggacggcat
     2281 cggccagctc ggtcaccgac aggcggccat cctcgacgag caggctgatg atcttgcggt
     2341 cgatcgcgtc cacgaccgta gatcctacga gatcaggccg ctccaggcag caggttcggc
     2401 agccattgcg ccaggttcgc cgccatcctg gcggtcatga tcctcctcgt cggcttcgtc
     2461 accgggctcg ccctggtcgc ccccatcggt ccggtcgccc tgaccctgtt cggtctcggt
     2521 gccgagcggg gtcgtcgggt cgcgctggcc ggcgccggcg gcgtggtgct cgctgacgcc
     2581 ctgaccatgc cggtcgcgct cgggagcgcc gggttcctgt cctcgctcga cagcggcatc
     2641 gtccgcggct tcgaggtcgt catgggcctg gcgctggtcg cgctcgcggt ctcgaccatc
     2701 gtcgccaccg accgggcccg ggccgccgtc gccgggatcc gtcgcccgac ccggacgctc
     2761 gcgttcatga ccatcgtgaa cccgctgtcg ctggtggcct gggccggcgt ggccctcgcc
     2821 ctgccgtcgt cgctgcggtc gccggccgcc ctcgtcgcct tcggcatcgg cctcgtgctc
     2881 gcctccgcgt tctggcacag cgggctggcc atggccgccg ggtcgctggc ccagcgcatg
     2941 ggcgacggac cccgccgggc cgtcacccgc gccagcggcg tcgtgctcct cgtggtcggc
     3001 ggcgtgctcg tcgcctgacc gtgcgcgccg atcggtccgt tcgttccacc cagcggtacg
     3061 aacggaccga tcggcgcgtt tgtaccggtg gtcacgggtg ggcggtgtcg gcctggacga
     3121 ggttgccgaa gcggtgcagc gtgcgggtca cggcctgctc gcgcaggtag cggcgcagct
     3181 cgaccccgcc gtcggcggtg accgggtcgt cgtcgaggtg gacccgggcc tcgatcgccg
     3241 cctgccggac cacgtcgctg gtggcgccga cgatgcgcac ccgctcgacg tcgaggtcgg
     3301 gcaggcgtgc cgcgaaggcg tcgtcgctct cgtcatcatg ggacgactcc accagcggca
     3361 ccccgcacgt ctcggctgcg tcccgcaccc gttcgactgc gacgtcgtcc gcgtccgcac
     3421 cgatgcgaag cccgacccgg gggagcgggc ggtaccgcaa gatgttggac tcgcagaaca
     3481 gcccggtcgg gtcgtggtcg atgccgtaga cggtccgcca ccaggcggcg tcgctgcgcg
     3541 tccacacgtc ccggtcgagg ggtccggtcg ggtgccaggt gccgagctgg acgacgtagc
     3601 ccgggccgcc ggccttggcg cccggtccga cgctggacgc cttccacccg ccgaagggct
     3661 ggcgctggac gatcgcaccg gtgatgcccc ggttcacgta ggcgttgccc acctcgacct
     3721 gctcggtcca cgcggccacc tcggccgggt cgagcgagtg caagccgccg gtgaggccga
     3781 aggcggagtc gttctggatg gcgatggcct cgtcgaggtc ctcggcccgg atcagtccga
     3841 gcaccggccc gaagcactcg gtctggtgga accacgaccc ccgacgcacg ccgatgcgga
     3901 ccccggggga ccacagctcg ccggagtcgt cgaggggccg gggctccacc agccactcct
     3961 cgccggggtc gagcgtggcg aacgcccggg cgagcctcgg gttcggctgc ccgatgagcg
     4021 gacccatcgt ggtcgtcggg tggtgaccgg gtcccacgtc gaggctctcc accgcgtcga
     4081 cgagctggcg ccggaagcgg gggctgtcgt acacctcgcc gacgcagatc gccaggctgg
     4141 ccgcggagca cttctggccg gcgtgcccga aggccgaggc gaccaggtcg gccaccgcga
     4201 ggtcgaggtc ggcgttgggc gtgatgacca tggcgttctt gcccgacgtc tcggcgaaca
     4261 ggcgcaggcc ggggtcccag gagcggaaca gctcggcggt gtcggtgccg ccggtgagga
     4321 tcacggcatc gaccgacgtg agcaggtggc ggcccacgtc gtcctcgggc acccggacgt
     4381 actggagcag gtcgaccggg accccggcgg cgtgggcgat ctccaccacc agctcgctgc
     4441 accggggcac ctcgggggcc ggcttggcca gcaccgcgtt ccccgccgcc agcgcggcca
     4501 gcatcccgcc ggcggggatg gcgacgggga agttccacgg cggcaccacg gcgaccacgc
     4561 cgaggggctc gaaccgggcg tggtcgcggg tcgttccggt ggggtccgcg aggtcgctgc
     4621 ggtccgcgta gtagcgggcg aagtcgatcg cctcgcagat ctcggggtcg gcctggccca
     4681 cggtcttgcg gccctcgtgg gccatgacgg tgagcaggtc gccccggcgg cgtgccatct
     4741 cgtcgcccac cgctcgcagg accgcccgac gctcctcggc gggccgggcc caccactcgc
     4801 cctgggcgcg ccgggcgcgc tcgatcatct cgtcgatcgc gctcgtctcg gtcgtcagcg
     4861 gggcgcgcac cgcttccggc gtgcgggcga ggtgctcgag cgcccaggcc cgggtgccgg
     4921 cgacggcggg atcggactcg ggctcgttgt cgaagggtgc caggccgacg ggctcgggcg
     4981 gctgggatcg gtccacggtc cggcgaggcg tcgtggagac gtcccaccgc tgggcgacgg
     5041 cgcggcggaa cgctgccgcc tgctcgacga aggacgccga gccgggctcg aggtcgccga
     5101 gcgcccgcat gaagttgccg accgcggcgt tctcctcgag ccgccggaag aggtaggaga
     5161 tggccacgtc gaagtcgtcg gggtcgacga cgggcgtgta gagcagcagc gagtcgagct
     5221 cgtcgcggat cacccgggcc tgcgcagggg ccatgccctc cagcatctcg atgcccaccc
     5281 ggtcggcgac gccccgacgc tgcgccagca gcgccgccca ggccacgtcg aacaggttgt
     5341 ggctcccgac gccgatgcgc acggcccgga gccgctcggg ctccaggacc cagtcgaggc
     5401 agcgcttgta gcccgcgtcg gtgtcggcct tggtgggatg cggggcctgg tcccacccgt
     5461 gcatggccgc gtccacccgc tccatcgcca ggttggcccc cttcaccagt cggatcttga
     5521 tgtcggctcc gccgcggtcc tgacgctcgc cggcccaggc caccagccgg ccgagggcgt
     5581 cgaaggcatc gggcaggtag gcctgcagga cgatgccggc ctcggtgccg aggaactcgt
     5641 cctcgtcgag cacccggcgg aacgcggcca tcgtcagctc gaggtcgggg tactcctcca
     5701 tgtcgaggtt cacgaacgtg ccccgggccc gggccgcccg caggatcgtg cgcagccgct
     5761 cggcgatgcg gtcgaggctg tcgtcccagg cccagtgctg gagctgggat gcgaccccgg
     5821 agatcttcac cgacacgtag tccacgtgct cgtcgccgag gaggtcgatg gtggcctgca
     5881 gccggcgggc ggcctcgcgc tcgcccagca cggcctcgcc gagcaggttc acgttgaggt
     5941 cgaagccctc ggcctggcgg gccccgaggt ggcggtccat ggccgccccg tgggcgtcga
     6001 ccaccaggtg cccgacgatc tggcgcatcc ggcgccgggc gaggggcatc accagccggg
     6061 gcagcaccgg tgccaggttc gcaccggccc gcagcagcac gcggtcgacg ggggagagga
     6121 accggggcag cgacgaccgt ccaccggcca gcgcgtgcag ctggtgggcc gcgacgtggt
     6181 ggtcgtcggg tcggatcacc cggtcgacga agcgcatggt gaaggccacg ccatcggggt
     6241 cgtcgacgag gcggtgcagc cggcgtgcgc tgcggcggtc ctcaccggtc tcggccgccg
     6301 ccgcggcgtc gagccagcgc aggacgacgt cgaccgacgc atcagccagc agcgccgcgt
     6361 cggcccgctc cgcctcgtgg tccaccggtt cgtcgagcgt ggccatgccc catggtgccc
     6421 cctcgcccgg agggcctgct cgtcaggtgg cgatcggccc cacgacggcg ccggcgaccc
     6481 gcaccaggtc ggccggggcc agcgagagct ccaggccccg ccgcccaccg ctgacgtgga
     6541 cctcgtcggc gaccgcgagg agctcgtcga cgaagacggg cagcggccgc cgggatccga
     6601 gcggggagat gccgccgacg acggagccgg tgagccgctc ggcctcggcc gggtcggcca
     6661 tggcggcgcg cttggcgccg gcgaggcggg cgagggcctt gaggtcgagc gtggcgctca
     6721 ccggcaccac ggcgagcacg tggcgggcct cgtcgaggac ggcgaccagg gtcttgccga
     6781 cggtgtcggg atcgaggtcg agcaccgtca cgaggccctc gccgtagccg cgctcggccg
     6841 ggggctcgta ctcggtggtg cggtagtcga cggccgcagc gtccagcgcg gcggtggcgg
     6901 gcgtctgcac cgtcgggcta cagcggcttg gggttgtccg ggtcgttctc ctcgatggcc
     6961 acgtgggcgt cgtccttgtc gcggacgcgg ccctcgtgct cggcgtcgag gtggagcttc
     7021 agctcccacc gcgccgagaa gcgcagctcg cagaacgggc actgggcggt ctcggtcatg
     7081 gacccttcct accgcggtgc gcccggtcgt gctggtcggc tcagacctcg tcgagccaga
     7141 cgggggcgcg gtcgagcagg aagtggctga ccgccaggca ccggtcgagc gtgtcgcaca
     7201 gctgctcctg gccgaggaac acccgtgcga gggagacgtc gaccttggcc acgatggcga
     7261 gggagcgctc gggcgcgtcg gtgaccgcac cgaaggagtc gatggcggac acctccagcg
     7321 gcaggtccgg gccgccgagg ccggccaggt cggtggccag cacgagcagg tcggggccct
     7381 tcggcagcgg cggcagcgac cacgtgaaga tgagcgggaa gtggaactcg tcgggcgggt
     7441 cgtcgtcctc gccgatggcg agcaccacgt cctcgaaggc cagcaggctg cgggggtcga
     7501 cgtcgagggc gaggtgcagg tcgagcgggc cgccgcaggc ctcctcgggg tggaggtcga
     7561 cctcccacgc ctgtcggagg gagtaggtct ccacgaagtg gcgctcgtcg tgcacgtgga
     7621 agccgtggtc gaccgcatgg tccttgaggt cggccacgaa cccggcgacg tcgatggagg
     7681 ccatggacca agcctgccac gacacccctg acagccctcg catcgccgtg gcgtgccgat
     7741 acggtcgacg cccatgcgcg acgacgacct cctcgccgtc ctccgggagg ccgccgtggc
     7801 ggtccgcgag gcgctcgacg gcctcgacga ctggggcctc gccggcaccc ggtcgggcca
     7861 gtaccgcagc gacctcgccg ccgacgacgc ctgcctggcg gtgctggacg acgccggctt
     7921 cggctggctc agcgaggagt ccggcgtgga gcacgccgac cgggagatca ccgtggtggt
     7981 cgacccggtc gacgggtcca cgaacgccag ccgcggcatc ccctggtacg ccacgagcct
     8041 gtgcgccgtg gacgccggcg gcccgcgggt cgcactggtc gtcaaccagg ccagcggccg
     8101 gcggtacgag gccgtgcggg gacgtggcgc caccgtcgac gggcgaccgg tcgccccctc
     8161 gggcgagacc gacctcggcc gttccgtggt cggcctgtcg gggtacccga accagtacct
     8221 cgggtggaag cagtaccggg cgctcggcgc ggcggccctc gacctgtgcg ccgtggccga
     8281 cggggtgctc gacggctaca tcgactgctc gccgagcgcc cacgggtcct gggactacgc
     8341 cgccgggatg ctgatctgcc gcgaagcagg catcgaggtg gtggacgcgt tcgaccgcga
     8401 gctgctcgat cccgccgccc tgcgccacgg cgaccggcgc acgcccgtcg ccggtgcgac
     8461 gcccgcgctc acggcggagc tggtcgatgc ccggcgtcgc ctgcgctggc ccgacgaccc
     8521 cgatccggcc ggtctcgtcg ccaccgaccg tcactaggct cgcaggcagt ggccggactc
     8581 ctgtatcgac tcgcgctcca ggtgtaccgc cggctgccgg ctgggcttcg ccgacgagtc
     8641 gtgcgctcga tctcgccgtc gttcacggtc ggcgccatgt gcttcatcga gcgggccgac
     8701 ggtgccctgc tgctggtgcg gcactcgtac cgggagcgct ggggcgtgcc cggcgggctc
     8761 ctgcagaagg gcgaggacgc cgaggtcggc gcccggcgcg aggtccgcga ggaggtcggc
     8821 ctggtggtgg agctcctcgg cgagccggcc gtggtggtcg acgccgaccc ccaacgggtg
     8881 gacgtggtct tccgggcccg cccggccgaa ggcaccgaca ccgaacccga cgtggccgat
     8941 tcggcggaga tcgtcgaggc ccggtggttc gcccccgacg cgctcccgga gctgcagttc
     9001 gagacggccg gggcgctggt tgccctggcc cgcatcgcca ccagcgccgg ccagcggccc
     9061 ctgcccgaac cggtctggcc ccgcttctag ctgtctcgct cgccgttccg ctgtcgctgc
     9121 cagcttcctg gggttctggc agcggccacg gaaccgccgg ctcccgattc ggacgggacg
     9181 tagggctcta gcccttcctg gcacggcctc ggacactggg agcccaggcc gtgcggaccc
     9241 cccgtcggcc gccgccccgg gaggtcggag ctgtcatgga acgcatcgtc gtcggaaccg
     9301 atggatcccc gggcggtgcg gacgcgatgc gctgggccgc cacccttgcg aggtcacagg
     9361 gcgcggagct ggtcgccatg acgtcgtgcg cgccggtgga agccgagctg ccaccggcgc
     9421 gcgccaccga gctgctcgac gagcagcgcg ctcacctgca ggagtggatc gacgccgcgc
     9481 acctcgggga cgtcgccgtg cggaccgtcg tggagcccgg tgaccaccgc cccggcatcc
     9541 tgcgactggc cctccgggag gaggccgacc tcgtcgtcgt cgggcgcgtc ggcagctcgg
     9601 ccggaccggg cctgctccac ctcgggagca tggccgagtg gctcgcccac cgagccgact
     9661 gcccggtcgc cgtcgtcggg ggcgccgtcg agctcgcccc gacccgcgtc gtggtgggcg
     9721 tcgacggatc cgccgggtcg caggcggcgg tcgactgggt gcgcacgctg ggccagccca
     9781 cgaccctgcg cgtcatcgcg ctgtcggtcc acgagccgct ggtcgaatgg acaccggcga
     9841 acagccccga gaactggcgt cgcggcctgg agcagcggat cgtcacggac atggcggcgc
     9901 ccctcttcga tgccgagatg gaggtggagg cgcgtgcctg cgccggaccg aacgtcgcgg
     9961 acgcgctgct ccaggtggcg gacgacgagg gcgcggacct cgtggtggtc gggacccgcg
    10021 gactcggcgg cttctccggg ctgcgggtcg gtggcgtggc cctgaaggtc ctccaccgag
    10081 cggaccggcc ggtcgtgctg gtgccgccgg ggtcatgacc gccgccgcga cgccggtgga
    10141 tgcgcacgac cgaggcgcga ccgagctcgc agccgccggt gcgcatgcgg cggaggtcga
    10201 tgcggtgctc gaggcgctcg gggcgagcga ctcggggctc acgtccgtcg aggcgacgcg
    10261 ccggctggcc gagttcgggc cgaaccgact gcccgacccc ccgcgggccg gcgtgctcgt
    10321 ccgcgtgctg cgccacttca acgacgtcct catctacatc ctcctggtcg cggcgggggt
    10381 caccgcggtg ctcgagcact ggatcgacgc cggcgtgatc ctggccgtgg tggtcgtgaa
    10441 cgcgctgatc ggctacgtcc aggagggcaa ggccgagcac gccctcgaag ggatccgcaa
    10501 gatgctgtcc ctcgaggccc aggtccgacg ggacgggcac tggacccgga tcggtgccga
    10561 cgagctggtt cccggcgacg tggtccgcct gcgctcgggc gatcgcctgc ccgcggacgt
    10621 gcggctgctc gaggcgacca acctccaggt ggaggagtcg gccctgaccg gtgaatcgct
    10681 gcccagcgac aagcgcaccg aaccggtcgc gcccgatgcc gggatcggtg accgtaccgg
    10741 gatggcgttc tccgggaccc tcgtcacggc aggtcggggc gtcggggtcg tgacggcgac
    10801 gggtccggcg accgagatcg gccagatcaa caccatgatc ggccaggtcc agacgctggc
    10861 cacgccgctg acgcgacaga tgcgggcgtt cggcacccag ctgtccgttg ccatcgtcgg
    10921 cctggccgtc gtggtgttcg tcctcggatt cgtcctgcac gactacccgg cgtccgagct
    10981 gttcctggcc gcgatcgggt tctcggtggc ggcgatcccg gagggcctgc cagccatcct
    11041 caccatcacg ctcgccatcg gcgtccagcg gatggcgggc cgcaacgcga tcacccgacg
    11101 tctcaacgcc gtcgagaccc tcggctcggt caccgtcgtg tgctcggaca agaccggcac
    11161 cctcaccaag aacgagatga cagctcggtg cgtcgtcacc cgcgccgggc actacgaggt
    11221 ctccggcacc gggtacgagc ccgcggggga gctctcgctg cacggcgagc acaccgccct
    11281 cgccgaccac gccgacctgc tcgcgctcgt ggaggcgatg gccgtggcca acgacaccga
    11341 catcgtcgag gtcgacggtc gctggacggt gaacggtgag cccacggagg gctcgctccg
    11401 cgcgctggcg accaagctcg gcttcgacgg ctccgggtac cgacggaccg cggtggtgcc
    11461 gttcgagtcc gagcacaagt tcatggcgac cgtcgacgag gtgcccggcg gtggtcacgt
    11521 cctgctcgtg aagggcgctc ccgaccgcct gctcgagcgc gctcggcgcg agctgggcgc
    11581 cgacggccgc ctcgcgccgc tcgacgtcgc atggtgggag tcccaggtcg agctgctcag
    11641 cgccgagggc ctccgagtgc tggctgcggc gcgctgcgac gacggcgggc cgcgcgacga
    11701 gctgtcgctc gaggacgtcc acggtctggt gttcgtcggc atcgtcggca tcgtcgatcc
    11761 gccccggccg gaggcgatcg agtcgatcgc gacctgcgga cgggccggca tccgggtcgt
    11821 gatgatcacg ggcgaccacg ccggcacggc ccgggcgatc ggacgggaga tgggcatcgg
    11881 cgacggcagt gcggccgtga ccggcaccga gctggaggcg gcatcggacg aggagctcca
    11941 ggcgatcgtg cgcacgaacg acgtgttcgc ccgcaccagc ccggagcaca agctgcgcct
    12001 ggtcacggcg ctgcaggcca acggcgaggt cgtggcgatg accggggacg gcgtcaacga
    12061 tgcaccggcg ctcaagcgcg ccgacgtcgg cgtcgccatg ggcatcaagg gcacggaggc
    12121 cacgaaggag gcggccgaga tcgtgctcgc cgacgacaac ttcgcctcga tcgagcgcgc
    12181 ggtggaggag ggccgcacga tctacgacaa cctccgcaag gcgatcttgt tcatcctgcc
    12241 cacgaacgct gccgaggcac tggtgatcgt cgtggcggtg gtggtcggtc tcgacctgcc
    12301 cctcaccccg acccagatcc tgtgggtcaa catggtgacc gcggtgacgc tggccctggc
    12361 gctggcgttc gagcctcccg agccggggat catggaccgg ccgcctcgtc ccaccggcgc
    12421 gtcgatcctg gatcggtact tcctgtggag gatcggcttc gtcggcgcgc tggtcggcgg
    12481 ggcgaccatc gccgtcttcc tcctgtccga ccgcagcgag gacagcctcg ccgtcgcccg
    12541 gaccgtggcg gtgaacaccc tcgtgttcgg ccagatctgc tacctgttca acagccggtt
    12601 cctgcgggag gccagctacc cgcccgctcg gctgctggcc aacccggtcg cctggatcgc
    12661 ggtggcggtg ctcacggcgc tccagctggg cttcgtgtac ctgccgttca tgaacaccac
    12721 gttcgggacc gcgccgctcg acctcgtgca ctgggtggtg ccggggctga tcggcatggc
    12781 ggtgttcggc gcggtcgaga tcgagaaggc gatcgcccgc cgacgggtcc gtccctagac
    12841 agccatggcc cggccctggc gctgggccgg ggagcagggg tggcggagct tggtgagggc
    12901 cttggcctcg atctgtcgga tgcgctcgcg ggtgagctcg aagtgacggc cgacctcgtc
    12961 gagggtgagc ggtcgctcgc cgacgaggcc gaagcgcagc tcgagcacct cgcgctcgcg
    13021 gtccgacagc gtcagcagca ggttgcgcag ctcgaccttc tcgaggttgg ccgctgcgac
    13081 gtcgaagggc acctcggcct tgtcgtcggg caggaggtcg ccgaactcgg tggcgtcctc
    13141 gccgatgccg gccgagagcg agatgacgtc ggcggtggcc tgcatcgcgg tctcgacccg
    13201 gtcgacgggc atgccggtga gctcgctcag ctcggcggcg gtgggctccc ggccgagctc
    13261 cttgagcagc gtgcccgacg ccttcgacag ggtggagagc gtgtcgacga cgtgggaggg
    13321 caggcggatg gtgcgtccct tgtcggcgat gccccggccg atggcctggc ggatccacca
    13381 ggtggcgtag gtcgagaact tgaagccctt gtggtggtcg aacttctcga ccgctcggat
    13441 cagcccgagg ttgccctcct ggatcaggtc gaggagcgga aggccggtgg actggtagcg
    13501 cttggcgatg gacacgacca ggcgcaggtt cgactggatg aaccgctgct tggccgccgc
    13561 cgcggcgcgg acctggcgcc gcagggacgc ccgcgccgcc ggcttcaggt cgagctcctc
    13621 ggccaggcgc agctcggcct cgcggccgtc ctcgatcgcc tgggccagct cgatctcctc
    13681 ggccgcggtg agcagcgggt actgcccgat gtcggcgagg tagcggccga cgaggtcgtc
    13741 aggaccccgt gccccccgat gaccgctgcg cttgggatcg gccacccttg acctccacgt
    13801 tgatcgatcg gcgccttgtg gcgtgttcga tgacgtgatc gacggtagcg acgcgcctgc
    13861 gagcgatcgt ggatgcctgc ggcgaggccg ctaccgttcc cccgctcggg cgcatagctc
    13921 agttggctag agcgcgtccc ttacaaggac gatgtcgggg gttcgagtcc ctctgcgccc
    13981 actcgcccgc acgacctatc ggccgttgac gctccagtgc cacggttcgc tcggaaggtt
    14041 gtagaacccg tagcgggagg cgttggcgtt cagccactgg aacgccgccg aggagcggga
    14101 gctgatgatc cggccgccgt aggtgaagtc gatggcgagg ccccgctcgt gcatcgaggt
    14161 ccccggacgg gcggtgggcg ggcggcagct gctggccggc atctcgtaga tcgcgtagtt
    14221 gctggtgccg cagttgttgc ggcgcacggc gatctggccg gctgggtcgc ggtagccgcc
    14281 gccggagagg gagatgccgg cggcatcggc ggcgccgagc atgtcggcca gcgagctggc
    14341 gatggtgttg tgcacccgga tgccgcgcac ggagacgatg ctcccggaac cggtgatggg
    14401 cacggtgcgg ggtggcgacg gcgcagccgg cgcgggcgca gccggggcgg ggcggggtgc
    14461 ggacgagcgg gactcgctgg ccgtccgggt cgctgccgcc gcccgggccg cggctgcggc
    14521 ctccgcctgc tcccgggcct gctgctggcg ctggagctcg gcggcgaggc ggcgctgctc
    14581 ctcctcgatc tgggcagcga gctcgcggtc gagctcggcg aggctctggg cctcggagag
    14641 ccggtggtcc aggcggtcct gcacctcggc gacgacggct tcctgctggc tggtggcctg
    14701 ctggacctct tcgagctggt tgccgaccgc agcctgctgc tcgcgggcct cgacggcggc
    14761 ggactcggcc tccgctcggg ccacctcgag gtcgtcccgg gcacgctcga gctggtcgag
    14821 gacgtcgaac tggtccgacg accgcgcctc gaggagcgcc tgcttgagct cggcctctcc
    14881 gatgctcgac gtggccaggg cgtcgaggat cccggccgac gcggtgggct gcatgtaggc
    14941 ggcgacgacc acgtccttgg cggcctggtc gaggactgcg atctcggcct gcttggactc
    15001 cacctgatcg gtggcggtct cgagcgcgac ctgggcgctg gccgcccggc gctcggcgtc
    15061 gcggaggtcg gcggtgcggg cggccacgtt gccctgcagg gcgcgcaggg cggcctcgac
    15121 ctgggcgtcg gtggcgcgga gcacgtcgat gtcggcggcg agggcggccc gcttgcgctg
    15181 gacctcgtcc cgctgggcct tggcgtcgga ctgcgcgccc gccggggcac cggccgcggc
    15241 gacggcgacg gccaggacga acacgaggag cacccgcagg gggcggcgga acaggtggtg
    15301 catggaggtg ggtccgaacg ggggcagcgg agtgtcagat gacggtcccg tcgcttccgt
    15361 catgggagcg taacgttccg gagccgtcct ccacccatgc ggaggcggct tcagcagcgt
    15421 tcgagcgcct gctggaggtc gccgaccagg tcgtccacgg cctcgatgcc cacggacagc
    15481 cggaccaggt cgtcggggac ctcgttctcg ctgccggcca ccgacgagtg cgtcatccgc
    15541 cccgggtgct cgatgaggct ctcgaccgcg ccgagggact cggcgagggt gaacacctgg
    15601 gtggactcgc acaggcggag cgcggcgtcg aggccgtccc ggagccggag gctgaccatc
    15661 ccaccgaatg cggacatctg gcgggcggcc acgtcgtggc ccgggtgcgc ggcgaggccg
    15721 ggccacagca cctcggccac cgcggggtgg tcgccgagca tcgtcgcgac ggcctcggcg
    15781 ttggcgctgt ggcgctccat gcgcaggtgc aacgtgcgac agccccggtg cacgaggtag
    15841 cagtcgaggg gaccggggac ggcgccggct gcgttctgca ggaacccgat ccgctcggcg
    15901 acctcgtcgt cgttcgtggc cacgaagccg ccgaccacgt cgctgtgccc gcccaggtac
    15961 ttggtggtgg agtgcaccac gatgtcggcg ccgaggtcga ggggctgctg cagggccggg
    16021 gtggcgaagg tgttgtcgac gacgagcagc gcacctcgct cgtgggcgac gtcggccagg
    16081 gcggcgatgt cggcgatccg cagccacggg ttcgtcggcg tctcggccca cagcagtcgg
    16141 gtgccgtcgg accactcccg gaccacggcg tcgacgtcgg agagccgggt cgtgcgcacg
    16201 acgagtccgg cgggtccgtg gacctgtcgg gcgaggcgga acgtgccgcc gtacgcgtcg
    16261 tcggacagga gcagcccgtc gcccggagcc agcagccgga gcacggcgtc ctcggcggcg
    16321 aggccggacg cgaacgcgaa cccgtgggcg gcgccctcga ggctggccag gtggcgctcg
    16381 agcgcggcgc gggtcgggtt gcccgaccgg ctgtactcga agccccgctg ctggccgacg
    16441 ccgtcctggg cgaaggtcga cgagaggctg atcgccggga ccaccgcacc ggtcacggcg
    16501 tcgggctcgc cggcgccgtg gacgcaccgg gtctcgaagg acgcgtcgtc gtcggtcggg
    16561 ctcatcgccg ctggtcctgg tcgctggtca tcgatcgcag tatgtcggcg cggctcacga
    16621 ccgcaaccgg gtgcccggca tcgaggacca gcgccgcaga cgcgtcctcg agcgtggcga
    16681 tggccgcgtc gatgggctcg ccgaggccga gggtggggaa cgccgggccg atcacgtcct
    16741 gcacgggccg ctcgaggacc gaggcatcgg ccagcacgtg gtcgaggagg ccgacctcgg
    16801 tgaccgagcc catgacctcg ccgaggacga cggggggttc ggccttcacg accggcagct
    16861 ggctcacccc gtactcgcgc atgatctcga tggcggtgcg gaccgtctcg tcgggatggg
    16921 tgtggaccag cagcggcagc tccgggtcgc gcccggtcag caggtcctcg accgtggggc
    16981 cggatgaccg caggaagccg tggtcggcca tccaccggtc gtcgtagagc ttggacaggt
    17041 agccgcgccc ggagtcgggg atgtggacca cgaccaccgc gtcctggggg aggtcgtggc
    17101 cgatgcgcag cgccgcggcg atcgcggtgc cgcccgagcc gccgatgagc aggccctcct
    17161 ggcgggtgac ccggcgggcg gcggcgaagg actcggcatc gctgatggcg acgatctcat
    17221 cggcgatgtc ggggtcgtag gtgctcggcc agaagtcctc gccgatgccc tccaccaggt
    17281 acggccgtcc gctgcccccg gagtacaccg agccctcggg atcggcgccg atgacccgga
    17341 tgtcggggtt ctgctccttg aggtagcggc cgatgccgct gatggtgccg ccggtgccca
    17401 cgccgcagac gaagtgggtg atgcgcccct tcgtctgggc ccagatctcc ggccccgccg
    17461 tgtcgtactg ggactggggg ttgttggggt tgtggtactg gtcgggcttc caggcgttcg
    17521 ggatctcgcg agcgaggcgg tccgacaccg agtagtacga ctcggggtgc tcgggatcga
    17581 cgctcgtcgg gcacacgacg acctcggcgc cgtaggcgcg gagctgggcg atcttgtcgg
    17641 tggccacctt gtcggggcag acgaacacgc agtggtaccc gcgccgtgcc gcgacgatgg
    17701 ccagacccac cccggtgttg ccgctggtcg gctcgacgat ggtcccgccc ggctggagca
    17761 ggccgtcgcg ctcggcggca tcgatcatcg ccagcgccgg tcggtccttg ctcgagaagc
    17821 ccgggttgaa cagctcgagc ttgccgagca ggtggcagtc caggtcccgg cccgtgcggt
    17881 cgaggcgcat catcggggtg ttcccgatga gcgcgaggag gtcgtcggcc acgtccatgt
    17941 cgccggccgg atcggtcagc cagcggtgcg gcacgctctg ggggagcgac acgtcgtcgt
    18001 cggtgtagac gaccgagggg agtccgtggt cgtgcaggtc gagcgccagc gagcgggcgg
    18061 tggcgccgtc gccgacgacg aggacgcgcc gggggacgtg ggtgcgcagg tcggcgatga
    18121 ggggcgccgt gtccgcgccc ggctggtggc gcaccacccg gcgcacccgg gcctgcaggc
    18181 gagcaggcac ctcgacatct ccgacggcaa cgagctggtc cacgggccca gtgtgcctgc
    18241 ccggagcatc gctcggccgg ggcggttgcg cgctgtcggg tccgcggtgg gcacaatcgt
    18301 gccgtgaacg agcccaccgc gaggacgccc gccgcccggg tgctgcgcaa cggtccccgt
    18361 cgactcttcc cgccggaccc ccgcaccgag gggtcggacc ccgactaccg cttctcgctg
    18421 gcgaacgagc gcacgttcct tgcctggatc cgctcctcgc tcgcgctggc cgccggcggg
    18481 ctcggcgccg tcgcgctgct ggacgacttc cccggcgagg agtacctcgg gctcgccctc
    18541 ctggccctga gcttcatcac cgccgggctg tcctaccgcc gctgggccct cgccgagacg
    18601 gccatgcggc tcggccgtcc gctgccgccg tcgcggctgc ccctcctcct cgccatcgcc
    18661 gtgtcggccg tggccgtcgc gtccgccgtg ctcgtcgccg tgaactcgac gtgagaccgg
    18721 agcaggtctt cgacccgctc ctccagcagg agcggacgtc gctggcgtgg gagcgcacgg
    18781 ccttcgccgg gatcgtcgtc ggctccctgc tgacccgggt cggcgccagc gtgcacgtgc
    18841 tgctcggcgg cttcgggctc ctccaggtct gcgcctcggc cggcctgctg gtctggtcgg
    18901 gccgtcacta cgaggacctc cacgtccagc tgcgcgccgg ggagacgccc acccacccgc
    18961 gagcggccat ggtcgtgggc gtcggggcca ccgtcgccac cgggctggcg acggcgctgg
    19021 ccctgctctt cgtcatcacc gactcgtagc tcccggcacg ccccggtcac ccgccccgcc
    19081 ggcgggacct gggaggatgg cgcccatggc gttcggtcag cagtccggtc ctccggccac
    19141 cgcccgacag gtgcagcagc tcaccgagct gctccacgcc gccgggcaca ccgacttccg
    19201 ggatgctcgc ggcccgctcg ggctcaccca gcgccaggcc ggcgggaagt tcacccggga
    19261 cgaggccgac gagctgatcg cccgcctcga gggcgaggcc gagggtgccg aggctccgcc
    19321 cggggaggtg ccgcggaccg cggcggccac cgaccgctac gaccgccggg aggcgcagcg
    19381 cctcgcggcg ctgcgccaga tgcccgacga ggccctcgcc gccgagctgc agcggcgggg
    19441 ctgggtggtc atggagccgt agggcgaggt ggctagacgc gctcgatgat gatggcgggg
    19501 gccatgccgc caccggtgca catcgtgatg aggccgaact ggccgtcgcg gcgctcgagc
    19561 tcgtcgagca cggtgccgat gagcatggcg cccgtggcgc cgatcgggtg cccgagggcc
    19621 atggcgccac cgttgacgtt gaccttgtcg cggtcgaggt cgaggtcgcg ctggaacttc
    19681 tcggagacca cggcgaaggc ctcgttgacc tcgaacaggt cgatgtcgtc gatggtcatg
    19741 ccggccttgg ccagcacctt gcgggccgcg ggcaccgggg cgttcagcat gagggtgggg
    19801 tcgtcgccga cggtggcggt ggccacgacg cgggctcgcg gcgtcaggcc gtgggccttg
    19861 gcgtagtcgg ggctggccca caggatggcg gcggcgccgt cgaccacgcc cgaggagttg
    19921 ccggcgtggt ggacgtgggc gatgtcgagg tccgggtact tctggttgat cagctcgcgg
    19981 aaggtcgggt tgccctcgtg gctgtagtcg gcgatcgccg ggaagctggg ggccagctgg
    20041 gcgagggact ccatggtcgt ctgcgggcgg gggaactcct cgtggtcgag ggcgaccgag
    20101 ccgtcgaggt tcttcaccgt cacgaggctc ttgtcgaagc ggccctcggc gatggcggcc
    20161 gcggcccggc gctgggactc cacggcgtgg gcgtcgagcg cctcgcggtc gatgccctcg
    20221 agcgtggcga tggcatccgc gcacacgccc tggtgcgact gcgggtgcag ctcctggagg
    20281 tgggcgttgc cggcgcccat cgggagcatg cccttgtgct tgatcgacat catctcggtg
    20341 ccgccggcga ccaccaggtc gtccatgccg gccatgatcc cgtgggcggc gatgttgttg
    20401 gccgtgatgc ccgagccgca gaagcggtcg agggtgacgc cgctggcctt ggtgtcgtag
    20461 ccggcgtcga gggcggacat gcggccgatg tcgccgccct gggggccgga ctgggcgctg
    20521 gtgccccaca ccacgtcgtc gacgtcggcg gtgtcgaagc cgttgcggtc caccagcgcc
    20581 tcgagcacgg tgcggcccag gtgctggggg tgcaggtggg cgagggcgcc cttcccctgc
    20641 ttgccgatgc cgcggggcgt gcggacggcg tcgatgatca gtgcgtcagg catggtggcg
    20701 ttcctctggt aatgggtgtt gccagattct ggcaccgatt gcgccggtgg ccccagtgcc
    20761 gcgcgtcctc gacggccggc cgcggacgag ccggttgacc gcacgaccaa aagaccgtac
    20821 atttccgtcg tggcgagtga cggtggtcac gccgacgacg accgacgggg gacgacgtgg
    20881 tgtcagcgat ccggctgagg tgcggcagcg accggtgggc gggtgcgcgc tcgtgacccg
    20941 cctgggcttc ctgctcgaca gcgacagctg catcggctgc cacgcctgca cggtcgcctg
    21001 caagagcgag cacgacgtgc cgctcggcgt gaaccggacg tgggtgaagt acatcgagac
    21061 cggcacgttc ccggacgtgg cccggaagtt cagcgtgatg cggtgcaacc agtgcgacga
    21121 cgcgccgtgc atgaagatct gcccgacctc ggcgctgtac cgcgacgaca agggggtggt
    21181 cgacttcgac gactccaact gcatcgggtg caagtcgtgc atgaacgcat gcccctacga
    21241 cgcgctgtac atcaaccccg agacgaacac cgcccacaag tgcaacatgt gcaatcaccg
    21301 gctggaggtg gggctcgagc cgtcgtgcca gatcgtgtgc ccgacgcagg ccatcaccat
    21361 cggtgacctg gacgacccga cgtccaagat cagccgcatc gtcgctcgtg acgacgtggc
    21421 cgtgcgggcg ccggagcaga acaccaagcc gaaggtcttc taccggggcg cggaccaggc
    21481 ctcgctcgac ccgacccgga ccgccatcgc gaacgacggg atgatctggg cggagacgac
    21541 gccgagccat cccacggtcc ccgtggcgct cggcaggaag cccaccgacg aggacggcgt
    21601 cgtcggacgc accgcgtaca ccaccgcaca ccacatgacc tggaagtcgc gcgtctcggg
    21661 ctacctggtc accaaggccg tggccgccgg cgtcatggcg gtcgccgcgc tgctcgtgct
    21721 gctcggtcac gaggacgtcc aggccgcggt cggggtggtg ccgcccgtgg tggccggcgt
    21781 gttcctggcc gccaccggct tcctgctcgt caccgacctc aagcagccca cccggttcca
    21841 ctacctgctc acccgcggca actggtcctc ctggctggtc aagggcgcct acgtgctcat
    21901 ggcgttcgcc gtggtgtgtg ggatgtggtt cctcgccggc gtcctcgacc agaccgacgt
    21961 gctgccctac ctggccccgt tcgccgccct cggcgccgcg gggacggccg ggtacaccgc
    22021 gtacctcttc gcccagtgcg aggggcgcga cctgtggcag acgccgctgc tgctgccggt
    22081 cctcctggcc caggcggtca ccgccggcgg tgcggtctac gccgtgctcg acgtcttcat
    22141 ggacgttccc gcccccaccg cgatccgctg ggtgctgctg ggtggcgtgc tcgccaccgc
    22201 agccctgctg gccgtcgagc tcacctcgca cggcagccgc cacgtcgagc tggccgtgca
    22261 cgccatgacc cgcggcacct gggccaagca gttctggtgg ggcggtgtgg tcctcggctt
    22321 ggtggtgccc ttcgtgctca ccgtcgcggc cctcgccgcc gacatcgacg cccctgccct
    22381 cgtcgccctg gccgggctcg ccgccctcgg cggcatgttc gcctacgagg acgccttcgt
    22441 ccgggccggc aagtccgtcc ccatctcctg aacgggagcc ccgcatgacc gagctgcaca
    22501 actacccacc gcacgagcag tggcacgact ggcgggagct cgacgccaag gcctggcccg
    22561 agaagctcga gcgcaagtac acgctcgtgc ccaccacctg cttcaactgc gaggcgggct
    22621 gcggcctggt ggcgtacttc gaccaggaga ccggcaagat ccaaaagatc gagggcaacc
    22681 cggagcaccc gggcagccgc ggccggaact gcgccaaggg cccggccacc ctcaaccagg
    22741 tctacgaccc cgagcggatc ctccacccga tgaagcgggc gggcgagcga ggcgagggca
    22801 agtgggagcg gatctcgtgg gaccaggcgc tcgacgagat cggcgcccgc ctccgccagg
    22861 cgatcgtcga ggaccgccac aacgagatca tgtaccacgt cggtcgaccg ggcgaggacg
    22921 gctacaccga ccgcgtcctg aaggcgtggg gcgtcgacgg ccacaactcc cacaccaaca
    22981 tctgctcgtc caacgcccgc atcggctacc agtcgtggat gggccacgac cggccctcgt
    23041 cggacttcgc gaacgccgag gtgatcttcc tcatctccgc ccacctcgag gccggccact
    23101 acttcaaccc gcacgcccag cggatcatgg aggcccaggc caagggggcg aaggtgatct
    23161 gcgtcgatcc gcgcctgtcc aacaccggcg ccaaggccga ccactggctg gcgccgtggc
    23221 ccggcaccga gccctacttg ttgctcgcca tcgcccgcat cctgatcgag gagggcacct
    23281 ggcacgagga gttcgtccgc cgctggacga actgggagac ctacctccag cgcacgcggc
    23341 ccgacctgcc cgtcgagtgg gagtcggtgc gcacggcgct gctcgacgag tacgccgagt
    23401 acacgcccga ggcggccgag cagaagaccg cgatcgaggc gggcaccatc cgagagctgg
    23461 ccgagctcat cggcgcccac ccgacgaagt tcgcctccca caactggcgc gccgcaggtg
    23521 ccgggcacct gggcggttgg caggtggccc gctgcctgtt cttcctgaac gtgctcaccg
    23581 gctcggtggc caccaagggc ggcaccgccg gcaacggcat gaacaagttc aagccggcgg
    23641 cgcccatcgg tgcgccgacg atcccgcgct ggaacgagct ggagtggccg accgagtacc
    23701 ccctgagcta ccacgagatg tcgatcctgc tgccccactt cctcaaggag ggtcggggtt
    23761 cgctggaggt gtacttctcc cgggtctaca acccgatctg gaccaacccc gacgggttca
    23821 cctggatgga ggcgctcacc gacaccgaca aggtccgctg ccacgtcgcg ctcacgccga
    23881 cgtggtcgga gacggcgtgg ttcgccgact acgtgctgcc gatgggcgtg tcggtcgagc
    23941 gccacgacgt gcacagctac gagacccacg ccggccgttg gatcggcttc cgccagtccg
    24001 cgttccggcg ctacgccgag atcaagaacg gcggtccgct cgacccgggc acccggagcc
    24061 acgagtacaa cccgggcgag gtgtgggagg agaacgagct ctggatcgac ctgtcgtggc
    24121 gcatcgaccc cgacggctcg ctcggcatcc gcaagtggtt cgagagcgag gaacgccccg
    24181 gtgagccggt gacggtcgac gagtactacg agcggatgtt caccgacagc gtgccgggcc
    24241 tggcgaaggc ggcggccgag gtgggccaga cgccgctcga gtacatgcgc gaccggggtg
    24301 cctacgccgt gcccaccgat ccctacgagc cctacgagcg tccggtcgac ccggccgacc
    24361 tcgacggctg cacgaaggac ggcgacggcg tgtaccgcaa gccgggcacg gccggcgcgt
    24421 ggtcgggtga cctcgacgag ctcgacgatc tgaagctggt gcccctcggc gacgggtcac
    24481 cggccgtcga ggtggacggc gaggccaagg agggcttccc gacgccttcc aagaagctcg
    24541 agctgttctc caccacgctc gccgactggg gctggcccga gtacgccacg cccaagtgga
    24601 tcccgtcgca ggtccactgg gaggacctcg acctggccgg caccgagcgc atcctgctgc
    24661 cgaccttccg catcccgacg ctcatccaca cgcggtcggc gaactcgaag tggttgaacg
    24721 agatcagcca ccgccatccg ctgtggatcc accctgagga cgcagagaag ctcggcatcg
    24781 aggagggcgg cctcgtgcgg gtcagcaccc gcatcggcca cttcgtcatc acggcgtggc
    24841 gcaccgaggg catccgaccc ggcgtcgtcg ccgcctcgca ccacatgggt cgctggcgcc
    24901 tcgacgagga caaggcccgg tcgtggggtg cggggaaggc ccgcatcgag cacgacgggt
    24961 cgacgtggaa gctggtccgg gagcacggca accagcccta cgagtcgagc gaccccgaca
    25021 ccgagcgcat ctggtggaac gacaccggcg tgcaccagaa cgccaccttc tgcgtccagc
    25081 ccgacccgat ctccggcatg cagtgctggc accagaaggt cgacgtcacg cccgcccaga
    25141 gcggcgacga gtacggcgac gtcgtcgccg acacggcgaa gtcgcgcgag gcctacctcg
    25201 agtggaaggc catgacccgg ccggcgacgg gcgatctccg ccgaccgctg tggtacgccc
    25261 ggccgctcaa gccgacggcc ggcgcctacc gccgaccggg cgcgtagcgc cggtcagtgg
    25321 ttgcgcaggg cgtcgatcag ctcgtccttg ctcatgtcgg agcggccttc gatgccgacg
    25381 tccttggcct tgtcgtacag gtcgtccttg gtccagtcct cgtaggccgg tgacgagcca
    25441 cccttctggc cggtgcggga gcggtcggag ttggcgatgc gggccgcctt ctccttgctc
    25501 atgccgtcgt ctcgcagggc ttcgtactgc tcgtcgtcct tgatgctgga accgtggtcc
    25561 ttggccatgt cgtccctcct gggtcgggtc gtgcccgtca cctacccgct ggcggtggcg
    25621 gcgatgaacc gtccccccga tcggccggcg acctgggtac ggtcagggga tgaccgatca
    25681 gcccggccac gtccgtgtga gcttcagcgc gcacctcgag gagatcgagc ggctggtcat
    25741 gcggcagttc ggcatggtca tcgagggcat cgccggcgcc accaccgtgc tgctcgccga
    25801 cgacgccgag gcggccaagc ggctcatcgc ccgggacgag atgatcgacg acctcgagta
    25861 ccagatcgag cagtcggtcg agcagcagct gctgctggag tcgccggtgg cccgcgactt
    25921 ccgcttcctg atgacggtgt cgcgcatcgt gcccgagctc gagcgcagcg gcgacctcgc
    25981 cgagcacgtc gccgccgagg ccgcccgcgg gttcggtcgg gcgctcaccc cgccgctgcg
    26041 cggggccgtg caggacatgg gcgacatcgc cggcgccatg tggcggcggt gcgccgatgc
    26101 gttccgcgat cacgacggcg aggccgccgt cgagctcgac cggaccgacg acgagctcga
    26161 cgccatccac cgccgcttcg tcgacgaggc cgccgagcag ctcaccggcg aggacgtgag
    26221 cagcgccgcg ctcgtcggtc ggtactacga gcgcctcggt gaccacgcgg tcaacatcgc
    26281 cgggcgggtc tgctacctgg ccaccgggcg cccgcagcgc aagaccgact agccgacgat
    26341 ctcgctggtc tgcatcaccg gctggatcga ggtgtagttg gccacgtcgg cgatgacggc
    26401 ctgcgtgccc tcgccgccca tggccgcgcc catcgcctcg agcgagtcga agctgaagtg
    26461 gacggcggcc tcgtacgggc cgtcgacacc cttgtcgatc tgggcgcccg agggctcgat
    26521 gccccacgtc tcacacgcca gcggcacgtg cttgtcgcgg tagtagtcgt ggtcgaactg
    26581 ggcaccttcg gtcttcgggt agagcacgct caggcggatc atcggtgggt ccttcggtcg
    26641 ggaatcggat tccggccgcg cagcctgacc gaccggtcaa gttgccgcaa ccgccatgta
    26701 tagggtcacg ccatgaccgg tgtcttggat cgtctgttcg gtgtcgaggg caagcaggtg
    26761 gtggtgaccg gcggcagccg gggcatcggc gccatgatcg cccaggggtt cgtcgaggcc
    26821 ggggcgcacg tcatcatctc ggcccgcaag gccgaggcgt gcgacgccac cgccgccgag
    26881 ctcgatgcgc tggagggctc cggctcgtgc acgtcggtcc ccgccgatct gtccacccag
    26941 gagggcgtcg acgccctcgc cgccgccgtg cgggagcgct gcggcgggag cctgcacgtc
    27001 ctggtgaaca acgcgggcgc cacgtggggg gagccgctcg acgagtaccc cgagtcggcc
    27061 ttcgacaagc tgttcaacat caacgtgaag ggcgcgttcc tgctgaccca ggccctgttg
    27121 cccgagctgc gggccgccgg caccgaggag gacccgagcc gggtcatcaa catcggatcc
    27181 atcgacggca tccgcacccc ggcgatggag acctacgcct acagcaccac caaggccggc
    27241 atcctcgccc tcacccgcca cctggccaag aacctcgtgc gcgaccacgt caacgtcaac
    27301 gccatcgccc ccggaccctt cgagtccaag atgatggcct tcgccctggg cagcgacgag
    27361 ggcaaggaga tggtggccag cggggtgccg atgcggcgca tcggccgccc cgacgacatg
    27421 gccggcgccg cgctgttcct ggccggtcgc ggtggcagct acctctgcgg cgagaccatc
    27481 gtcgtcgacg gcggcatcgc caacgtgggt tagctgtcag agccagctcg gttccctgcg
    27541 cgcagatcga ccgccagcgg tcgatctgcg cgtcaggaac gcatcaggag taggcgacct
    27601 ggcagaaccc gtggttgcag tagccgcggg ggttcttgtg caggtactgc tggtggtagt
    27661 cctcggcgta gtagaagacg ccgtcgccgg catctgacga gcgggcgatc tcggtggaga
    27721 tgtcgccgaa gccctgctcg tgcagcgccg cctggtactt gtcccgcgac gccagcaccg
    27781 cctcgtgctg tgcgtcggtg gtggtgtaga tcgccgagcg gtactgggtg ccgacgtcgt
    27841 tgccctggcg gttcggcgtc gtcgggtcgt ggttctccca gaacgcctgc aggagcacgt
    27901 cgatgtccac gacgctcggg tcgtacacca cctgcacgct ctcggtgtgc ccggtcatcg
    27961 ccgtgcacgt ctcctcgtag gtggggtgct tcgtccagcc gcccatgtag ccggcggcgg
    28021 tggagtgcac gccctcgatc tgccagtaga tgcgctcggc tccccagaag cagcccatcg
    28081 ccacgtacag cacctcgtgg ccgtcgggcc acggtccctc catcggggtc ccgagcacga
    28141 cgtgggtgtc ggacacgggg atgggctggt cccgacccgg gatggcgtcc tcggggctga
    28201 ccatcgtcgt cttgtcacgg ccgaagagag gcatggcccg aggctagggc gacgggcccg
    28261 actccggacc gcgacgccga ccgcgccggt atcgtgcggc cgtgctcgag gatcacgagg
    28321 tcgaacgcat cctggtcgtg acggcccatc ccgacgacgt cgacttcggc gtcgccggct
    28381 cggtggcccg gtggacggcg gcgggcgccc acgtcacgta ctgcatcctc accgacggcg
    28441 acgcgggtgg cttcgacccc gacgtgccac gaggggacat cgccggcatc cgccgcggcg
    28501 agcagaccaa cgccgccacc caggtcgggg tcgaggacct gcacttcctc ggctaccccg
    28561 acggccgcct cgaggtcagc ctcgacctgc gccgcgacat cagccgcatc atccgcgagg
    28621 tccgtccccg gcgcgtcgtc acccagtcgc cgctgcggac gatggacagc gtctaccgga
    28681 accacccgga ccacctggcc tgcggggagg cgacgtgctg cgcggtgtat cccgactccc
    28741 gcaacccgtt caccttcacc gagctgctgg ccgagggcct cgaaccgtgg accgtcgagg
    28801 agctgtgggt catgggtacc gtgcccggcg cgcaggaggc cttcgtcgac atcaccgaca
    28861 ccttcgaccg caagatcaag gcgctgctgt gccacgtcag ccagatgacc gacccggacg
    28921 ggatcgagga ccggctgcgg gagtggaacg gcgccaacgc cgaagccggt ggcctgcccg
    28981 ccggcaccct cgccgagcgc tacgtcgtga tgcccaccgt gtgagctctc ggttctgggg
    29041 tcaggccagg ccggcggcgc ggagcatcga gggtgggatc gacgtgtccc cccgggcgag
    29101 catggcggcc cgttcgacgt tgatctcgtc gtgctcccgc tggcgccact cgtcccacgg
    29161 ccccgccgag atcgcggccg ggtcgccggc ggcggcgacg cacgcccgca ggtagccctg
    29221 cagctcgcgc acctcctcct cgccaccgat gcggccgtgg ccgggcacga tgatcggcgc
    29281 cagctcgagg atgcggtcga gctcgtcggc ccaccgggcg gggtcgccct ggtagcagcg
    29341 cggcgtgacg ccgaagctgg ccatggcgcc ggcgaagcag atgccggcgg cgtcgaccac
    29401 ggcgacgagg ttctcctcct gcgctccgga gcacgggtgc aggctgaccg ccggggtgag
    29461 ctggacgcgt tcgtcgacga tgtggctgac cggcttggtg gtgagctcgt cgtcgaactc
    29521 gccggccacg tcgggcagca gccggcggta gatgtcgggc gtcggtggct ggtcgaggtg
    29581 gacgctggtc tggcggcgcc cgtacatgcc cgacatggtg aacagcgccg atccgccgac
    29641 gaactcgatg gtcgatgccg tcagcaccgt gcggcggacc cgtaggccca gcgcatcgat
    29701 ggcggcgacg aaggcggcgg tctgcgacgg cacgcagagc gtgtcgatcg ccgtggccgc
    29761 atccgcatcg acgaccaggc cggcgttggg ccggccatca cccgggtcct cggcgagcca
    29821 ggcgaggatc ccgtcgtcga gggtgaccag gctcacgggc cgacctcgtc gagggtgagc
    29881 gacatcggca ggtggaggat gccgtcctcg aggtaccggg ggccggagac gacgaac
//
LOCUS       DATISU010000120         8549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_331622, whole genome shotgun sequence.
ACCESSION   DATISU010000120 DATISU010000000
VERSION     DATISU010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8549
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331622"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VLR27_05040"
     CDS             complement(<1..332)
                     /locus_tag="VLR27_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP02842.1"
                     /translation="MPDSTAAARSGTPTPSPADPDRQLTSQGRERKQQLVDCAAQLFT
                     ERGFAETRIKDIVEAAGVAKGLFYWYFENKEAVFRELVELTRQRMRQQQKRDIDPAAN
                     PLLRLRQGT"
     gene            406..726
                     /locus_tag="VLR27_05045"
     CDS             406..726
                     /locus_tag="VLR27_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HSP02843.1"
                     /translation="MEGTVSSASNRTERMANRARNPKWLNPALYIDMSICINCDTCIR
                     HCPPHFGAIFNHGIDVVIIPELCSGCLKCVDPCPVDCIHPVEDGMETPLDWWDAPLED
                     DPYR"
     gene            855..1649
                     /locus_tag="VLR27_05050"
     CDS             855..1649
                     /locus_tag="VLR27_05050"
                     /inference="COORDINATES: protein motif:HMM:NF028286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcpC/CpaB family pilus assembly protein"
                     /protein_id="HSP02844.1"
                     /translation="MKKKRASLIGAVLLVALGTFALVKYVSTAEDRALAGEELVDVLV
                     LDAAVPAGTAANELQQYLRVESVPVKVQTGGALASLSEADGMVTAVDLVEGEQLTVSR
                     LVDATEFNALQARVGRGGGTVAVPEGLLEITIPLDPIRAVGGTVRPGDLVGLIASFEQ
                     FQSDGAVGPDGQPVAGESVSGFLLHKLVVTNVQGAPAVAAAAQESSDPAAAAPTGQLY
                     VTLALDAAAAERVVYASKYGDIWLAVEPADADESGTSVQTADRIFG"
     gene            1687..2478
                     /locus_tag="VLR27_05055"
     CDS             1687..2478
                     /locus_tag="VLR27_05055"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSP02845.1"
                     /translation="MATDPSRIITILSPKGGAGKTSSATNLAVGLAKRYPREVVLVDL
                     DLQFGDVASNMRLQPTTTILDLARAWPVDSAQVKLALTKHSSGVYTLCAPLSPAEADE
                     ITADHVTGVLRALHRSFRFVVVDTDPGLSERVLSALDVSTDMVMICSTEVPSIRGLKK
                     ALEALDVVGLTSARRVLVLNRSNAKVGVDIGQLERTIGRPVDVRVPSSIDMVLATNDG
                     IPIMESGRNPDLVRAFEHLVEQFVTDEGDQHHGGKPASRLFKRKG"
     gene            2482..3885
                     /locus_tag="VLR27_05060"
     CDS             2482..3885
                     /locus_tag="VLR27_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HSP02846.1"
                     /translation="MKLGERVRAATEGDFELEFPDLSFEQVTADHASNVAMTAALMES
                     ESFSGLRQKVQDALLLALGNRVGEPDVDDDELKQMARAEMVKVLASEDIPLAEDERQR
                     LVDVIAADLLGYGPIQPLLSDDSVSEIMVNGTRSIYIEKDGKLQVASINFSDEDSLRK
                     VINRIVAPLGRRIDESSPMVDARLPDGSRVCAVIPPLAVDGSSLTIRKFAAKKFTAED
                     LIEKGSATVESLYMIAACVHAKLNILVSGGTGSGKTTLLNVLSSFIPDGDRIITIEDV
                     VELQMRQQHVVRMETRPGNSEGRGEISIRDLVRTALRMRPDRIVVGECRGGEALDMLQ
                     AMNTGHAGSLSTLHANTPRDAMTRLETMVLMAGIEFPMQAIRQQISGAIDLVVQIGRL
                     RDGSRRIVQICEIGRVVGDDIEVLPIFDFDYSPGLDPSGRVLGVLRPTGYRPRFIEHI
                     KELGIEFEERIFGGNQE"
     gene            3882..5783
                     /locus_tag="VLR27_05065"
     CDS             3882..5783
                     /locus_tag="VLR27_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSP02847.1"
                     /translation="MSARSRTARLLAGLLALVALLVAGAASPVAAQSEDIEVRSIDVN
                     GFPLVRAVVSGPTVTGDPEAAFTVVEDGEEQNVDVVRLRTNDLNLVVAIDVSGSMAGE
                     PIEQAKAAVADLLQTLPADVPVSLVSFGESAVIQHAFSTDRASLQGAVDGLIAAGGTA
                     LFDGIDRSITQLASRGGTGALVVLADGADSASTGTADAIVERFDSTPIAASFVALQTP
                     DQDLAALQTIADSAGGQVVAVEQSGQFREVFTNIAAGLGGLFELRWTSASEGESADLT
                     IAMADAPDVAVSTTFEHPAVRQFVPDVADETRVVPGAGVLSGPWVPIAAALGVSLGAF
                     LLLNGMRSSEQAVERREQRRQQKDLSKAQKTKRKSGVQERLEGISAQVLEKNNRGRSL
                     DDTLDRAGLSMRSTEFVVVCLAVSIAALVVGVLLFGVPYFLLMAIPIGLGIPMFLKFK
                     IGRRRKQFVNQLGDTLQLMAGSLTAGYGLGQTLDSVARETDSPTSEEFQRVVMETRLG
                     RSLEDALQAMSYRIGAKDFDWVVDAIKIQASVGGNLSEIIEQVGETIRARTRIKRQVD
                     ALTAEGKISALVLFVLPFGLFGFIFSSNPDYIKPMFSSGIGQAMLGAAGVMLACGGLW
                     LKKLVNPEF"
     gene            5786..6685
                     /locus_tag="VLR27_05070"
     CDS             5786..6685
                     /locus_tag="VLR27_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSP02848.1"
                     /translation="MGIVYLAALFVIGGLGLAFWALKPEPKVIAIGMDPEDVVPTSRE
                     LELQGSFLTRVVNPLMQGLANKFGRYLPTGLIDARAKTLRVADLDKRWRPEVILGMQI
                     AGFALGLFGASTYLSSAGASRTNLIVAGLMVLLLTMAPDAKIKRMADDRRKAITNELP
                     DVLDQLTVTVEAGMSFDGAVLRAGQEGRGILATELLKAVNDMQLGLSRGEALQAMSDR
                     TDVADLRQFVTAVRQAEKHGFPLANILRLQALELRDKRRARAEERAMQVPVKITFPLV
                     FCILPALFVVILGPAAVNISGGF"
     gene            6703..7449
                     /locus_tag="VLR27_05075"
     CDS             6703..7449
                     /locus_tag="VLR27_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSP02849.1"
                     /translation="MPDVLPEAEPLSVDGGPVGCLVIHGFTGNPSSMRLIAEAMADAG
                     HSVEMPRLPGHGTTVEDMNTTSWADWTAEVEAAYQRLAGRCDHVVAMGLSMGGTLTGW
                     VGAEHPEIAGLVFINAAAERSDEMRGGLQAMLDAGETYMDGIGSDVADPEVTESAYAK
                     VPLAPLVSMLDGVADLQDRLAGITAPALIMTSPEDHVVAPTASDHLAATLGGSVERVT
                     LERSYHVATIDHDRDLIIERIRDFVARVTA"
     gene            7493..7795
                     /gene="gatC"
                     /locus_tag="VLR27_05080"
     CDS             7493..7795
                     /gene="gatC"
                     /locus_tag="VLR27_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HSP02850.1"
                     /translation="MPGHITSDDVAHVARLARLTLTDDELDTFTGQLASILDHAADVE
                     ALDLDDVPPTSHPYPLENVVRPDEVRPCLDRDEVLAEAPSAEDGMFRVPPVLGEAP"
     gene            7792..>8549
                     /locus_tag="VLR27_05085"
     CDS             7792..>8549
                     /locus_tag="VLR27_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HSP02851.1"
                     /translation="MSPVDRFQTAAGLAAAVATGERSARSVLDEHLARVDEREGEIHA
                     FNHLMADEARARADDIDARIAAGEDVGPLAGVPVALKDNLCTRGIPTTCSSKILDDWR
                     PPYDATVVNRLRDAGAVVIGKTNLDEFAMGSSTENSAFGPTRNPLDTSRVPGGSSGGS
                     AAAVAAGFAPISLGSDTGGSIRQPAALCGVVGAKPTYGRASRYGLIAFASSLDQIGPF
                     AHTVEDAALLMEVVGGHDPLDATSIPEPFPDLRST"
BASE COUNT         1265 a         3257 c         2776 g         1251 t
ORIGIN      
        1 gtgccctggc ggaggcgcaa gagcgggttg gcggcgggat cgatgtcgcg cttctgctgc
       61 tggcgcatcc gctggcgggt gagctcgacg agctcgcgga agacggcttc cttgttctcg
      121 aagtaccagt agaagaggcc cttggccacg ccggcggcct cgacgatgtc cttgatccgg
      181 gtctcggcga agccccgctc ggtgaacagc tgtgccgcgc agtccaccag ctgctgcttg
      241 cgctcgcggc cctgggacgt cagctggcgg tcggggtcgg cgggcgaggg cgtgggtgtg
      301 cccgagcgcg cagcggcggt cgagtcgggc acccgcagag tgttccgcca ccttgaccac
      361 catgtcaagc accgcttccg ggaccggtgc taccgtcggc gggcgatgga ggggaccgtc
      421 agcagcgcca gcaaccgcac cgagcggatg gcgaaccgcg cccggaaccc caagtggttg
      481 aacccggcgc tgtacatcga catgtcgatc tgcatcaact gcgacacctg catccggcac
      541 tgcccgcccc acttcggcgc gatcttcaac cacggcatcg acgtggtgat catccccgag
      601 ctgtgctccg gctgcctgaa gtgcgtcgat ccctgcccgg tcgactgcat ccacccggtc
      661 gaggacggca tggagacgcc gctcgactgg tgggacgctc ccctggagga cgacccgtac
      721 cggtaggccc cccggctcgc cgcttccggt tccgccggaa ccgctgagaa ggtcgtcacc
      781 ggaaactgct tcagattctc cgacgacggc cgatgactcc tgaggagcag cctcgcgaac
      841 cctcgggaga gaacgtgaag aagaagcgcg caagtctcat cggtgccgtc ttgttggtgg
      901 cactgggcac cttcgcactg gtgaagtacg tgagcacggc cgaagaccgt gccctggccg
      961 gcgaggagct ggtggacgtg ctcgtcctcg acgctgccgt gccggccggc accgcagcca
     1021 acgagctgca gcagtacctg cgggtcgagt cggtgccggt gaaggtgcag accggcggtg
     1081 cgctggccag cctgagcgag gccgacggca tggtcaccgc cgtcgacctg gtcgagggtg
     1141 agcagctgac cgtcagccgc ctggtggacg ccaccgagtt caacgccctc caggcccgcg
     1201 tcggccgggg cggcggcacg gtggcggtcc ccgagggcct gctcgagatc accatccccc
     1261 tcgacccgat ccgggctgtg ggcggcacgg tgcgcccggg cgacctggtc ggcctgatcg
     1321 cctccttcga gcagttccag agcgacggcg ccgtcggccc cgacggccag cccgtcgccg
     1381 gcgagagcgt gtcgggcttc ctgctccaca agctggtcgt gaccaacgtc cagggtgcgc
     1441 ctgcggtcgc cgctgcggcc caggagagct cggacccggc agccgccgcc ccgaccggcc
     1501 agctgtacgt gacgctggcc ctcgacgccg ccgccgccga gcgggtcgtc tacgcctcga
     1561 agtacggcga catctggctg gccgtcgagc ccgccgacgc cgacgagtcg gggacgtccg
     1621 tgcagaccgc cgaccggatc ttcggctgag atgggagcca cgacgttgac ctctccaccc
     1681 gagggggtgg cgaccgaccc cagccggatc atcacgatcc tgtcgcccaa gggcggcgcg
     1741 gggaagacgt cgtcggccac caacctcgcc gtgggtctgg ccaagcgcta cccccgtgag
     1801 gtggtgctgg tcgacctcga cctccagttc ggtgacgtgg cgtccaacat gcgcctccag
     1861 ccgaccacca cgatcctcga cctggcccgg gcctggccgg tggacagcgc ccaggtgaag
     1921 ctggccctga ccaagcactc gtcgggcgtc tacaccctgt gcgccccgct cagcccggcc
     1981 gaggccgacg agatcaccgc cgaccacgtc accggcgtgc tccgggcgct gcaccggtcc
     2041 ttccggttcg tcgtcgtcga caccgacccg ggtctgagcg agcgggtgct ctcggcgctc
     2101 gacgtctcga ccgacatggt gatgatctgc tccaccgagg ttccctcgat ccgtggcctc
     2161 aagaaggccc tcgaggcgct cgacgtggtc gggctcacct cggcccgacg ggtcctggtc
     2221 ctcaaccgga gcaacgccaa ggtcggcgtg gacatcggcc agctcgagcg caccatcggc
     2281 cggccggtcg acgtccgcgt gcccagctcg atcgacatgg tgctggccac caacgacggc
     2341 atcccgatca tggagtcggg ccgcaacccg gacctggtgc gggccttcga gcacctcgtc
     2401 gagcagttcg tcaccgacga gggcgaccag caccacggcg gcaagcccgc ctcgcgcctg
     2461 ttcaagcgga agggctgagc gatgaagttg ggagagaggg tccgggccgc gacggagggg
     2521 gacttcgagc tcgagttccc cgatctgtcc ttcgagcagg tcaccgccga ccacgcatcc
     2581 aacgtggcga tgaccgccgc gctcatggag tcggagtcct tctccgggct gcgccagaag
     2641 gtgcaggacg cgctgctcct cgccctcggc aaccgggtcg gtgagcccga cgtcgacgac
     2701 gacgagctca agcagatggc ccgggccgag atggtcaagg tcctggcctc cgaggacatc
     2761 cccctcgccg aggacgagcg ccagcgcctg gtcgacgtca tcgccgccga cctgctgggc
     2821 tacggcccga tccagcccct gctctccgac gactcggtgt cggagatcat ggtcaacggc
     2881 acccgctcga tctacatcga gaaggacggc aagctgcagg tcgcgtcgat caacttcagc
     2941 gacgaggaca gcctgcgcaa ggtcatcaac cgcatcgtgg ccccgctcgg ccgtcgcatc
     3001 gacgagtcct cgccgatggt cgacgcccgc ctccccgacg gatcccgcgt ctgcgccgtg
     3061 atcccgcccc tcgcggtgga cggctcctcg ctgaccatcc gcaagttcgc ggccaagaag
     3121 ttcacggccg aggacctgat cgagaagggc tcggccacgg tcgagtcgct ctacatgatc
     3181 gccgcctgcg tccacgccaa gctgaacatc ctggtcagcg gcggcaccgg ctcgggcaag
     3241 acgaccctgc tgaacgtgct gtccagcttc atccccgacg gcgaccgcat catcacgatc
     3301 gaggacgtcg tcgagctgca gatgcgccag cagcacgtgg tccgcatgga gacccgcccg
     3361 ggcaacagcg agggtcgtgg cgagatctcg atccgcgacc tggtccgcac cgccctgcgc
     3421 atgcgacccg accgcatcgt cgtcggcgag tgccgcggcg gggaggccct ggacatgctc
     3481 caggccatga acaccggcca cgccggttcg ctgtccacgc tgcacgccaa cacccctcgc
     3541 gacgccatga cccgcctcga gacgatggtg ctcatggccg gcatcgagtt cccgatgcag
     3601 gccatccgcc agcagatctc cggcgccatc gatctggtcg tgcagatcgg ccgcctccgc
     3661 gacggcagcc gccgcatcgt gcagatctgc gagatcggtc gcgtcgtcgg cgacgacatc
     3721 gaggtcctgc cgatcttcga cttcgactac tcgcccggcc tcgatcccag cggccgcgtg
     3781 ctcggcgtcc tgcgccccac cggctaccgg ccccgcttca tcgagcacat caaggagctg
     3841 gggatcgagt tcgaggaacg catcttcgga gggaaccagg aatgagtgct cgatcccgca
     3901 ccgcccgcct cctcgccggc ctgctcgccc tcgtggccct gctggtcgcc ggcgccgcct
     3961 cgccggtcgc cgcacagtcc gaggacatcg aggtgcgcag catcgacgtc aacggcttcc
     4021 ccctcgtgcg cgctgtggtc tccggaccca cggtcaccgg tgacccggag gccgcgttca
     4081 ccgtcgtcga ggacggcgag gagcagaacg ttgacgtggt gcgcctgcgc accaacgacc
     4141 tgaacctggt cgtggccatc gacgtctccg gctccatggc cggcgagccc atcgagcagg
     4201 ccaaggccgc cgtggccgac ctgctccaga ccctgccggc cgacgtgccg gtgtcgctgg
     4261 tgagcttcgg cgagtccgcc gtcatccagc acgccttctc caccgaccgg gcgtccctcc
     4321 agggcgcggt cgacgggctc atcgccgccg gcggcaccgc gctgttcgac ggcatcgacc
     4381 gcagcatcac ccagctcgcc tcccggggcg gcaccggtgc gctcgtcgtc ctcgccgacg
     4441 gcgccgactc cgccagcacc ggcaccgccg atgcgatcgt ggagcgcttc gactccacgc
     4501 cgatcgccgc ctccttcgtc gccctgcaga cgcccgacca ggacctcgcc gcactccaga
     4561 cgatcgccga ctccgccggc ggccaggtcg tcgccgtcga gcagtcgggc cagttccgcg
     4621 aggtgttcac caacatcgcc gccggtctcg gtggcctgtt cgagctccgc tggacctcgg
     4681 cctccgaggg cgagagcgcc gacctgacga tcgccatggc cgatgcaccc gacgtcgccg
     4741 tcagcaccac cttcgagcat cccgccgtgc gccagttcgt gccagacgtc gccgacgaga
     4801 cccgggtcgt gcccggcgcc ggcgtcctca gcggcccctg ggtgccgatc gccgccgccc
     4861 tcggcgtcag cctcggtgcc ttcctcctgc tgaacggcat gcgcagctcc gagcaggccg
     4921 tcgagcggcg tgagcagcgc cgccagcaga aggacctgag caaggcgcag aagaccaagc
     4981 gcaagagcgg ggtgcaggag cgcctcgagg gcatctcggc ccaggtgctg gagaagaaca
     5041 accgcggtcg cagcctcgac gacaccctcg atcgagccgg tctgtcgatg cgctccaccg
     5101 agttcgtggt ggtctgcctc gccgtctcga tcgccgccct ggtcgtcggc gtgctgctct
     5161 tcggcgtgcc gtacttcctg ctcatggcca tccccatcgg cctcggcatc ccgatgttcc
     5221 tcaagttcaa gatcggccgc cggcgcaagc agttcgtcaa ccagctcggc gacaccctcc
     5281 agctcatggc cggctcgctg accgccggtt acggcctcgg ccagaccctg gactcggtgg
     5341 cccgggagac cgactcgccc accagcgagg agttccagcg ggtcgtcatg gagacccggc
     5401 tcggccgcag cctcgaggac gccctccagg ccatgtcgta ccggatcggc gccaaggact
     5461 tcgactgggt cgtcgacgcc atcaagatcc aggccagcgt cggcggcaac ctgtccgaga
     5521 tcatcgagca ggtcggcgag accatccggg cccggacccg catcaagcgc caggtcgacg
     5581 ccctcaccgc cgagggcaag atctccgccc tcgtgctgtt cgtcctgccc ttcggtctgt
     5641 tcgggttcat cttcagctcg aaccccgact acatcaagcc gatgttctcc tcgggcatcg
     5701 gtcaggcgat gctcggcgcc gccggcgtca tgctcgcctg cggcggcctc tggctgaaga
     5761 agctcgtgaa cccggagttc tgatcatggg aatcgtctac ctcgcagccc tcttcgtcat
     5821 cggtggcctc ggcctcgcct tctgggcgct caagccggag ccgaaggtca tcgccatcgg
     5881 catggacccc gaggacgtcg tccccacgtc ccgtgagctc gagctccagg gctcgttcct
     5941 cacccgggtc gtgaacccgc tcatgcaggg gctggccaac aagttcggcc gctacctccc
     6001 gaccggcctg atcgacgccc gggccaagac cctccgggtc gccgacctcg acaagcgctg
     6061 gcggcccgag gtcatcctcg gcatgcagat cgccggcttc gccctcggcc tgttcggcgc
     6121 gtcgacctac ctgtccagcg ccggcgccag ccgcaccaac ctcatcgtcg ccggcctcat
     6181 ggtcctcctg ctcaccatgg cccccgacgc caagatcaag cggatggccg acgatcgccg
     6241 caaggccatc accaacgagc tgcccgacgt cctcgaccag ctcaccgtca ccgtggaggc
     6301 cggcatgtcc ttcgacggcg ccgtcctgcg ggccggccag gagggccggg gcatcctcgc
     6361 caccgagctg ctcaaggccg tcaacgacat gcagctgggc ctgtcccgcg gcgaggccct
     6421 ccaggccatg tccgaccgca ccgacgtggc cgacctgcgc cagttcgtca ccgccgtgcg
     6481 ccaggccgag aagcacggct tcccgctggc caacatcctg cgcctccagg ccctcgagct
     6541 gcgtgacaag cgccgggccc gggccgagga gcgggccatg caggtccccg tgaagatcac
     6601 gttccccctg gtgttctgca tcctgccggc cctgttcgtg gtcatcctcg gccccgccgc
     6661 cgtcaacatc tccggcggct tctgaggcgt agcctccggg ccatgcccga cgtcctcccc
     6721 gaagccgaac cgctctccgt cgacggcggc ccggtcggct gcctggtgat ccacgggttc
     6781 accggcaacc cgtcatccat gcgcctcatc gccgaggcca tggccgacgc cggccacagc
     6841 gtggagatgc cgcgcctgcc cggccacggc accacggtcg aggacatgaa caccacctcg
     6901 tgggccgact ggaccgccga ggtcgaggcc gcgtaccagc gcctcgccgg ccgctgcgac
     6961 cacgtcgtgg ccatgggcct gtccatgggc ggcacgctca ccggttgggt cggggcggag
     7021 cacccggaga tcgccgggct cgtgttcatc aacgcggctg ccgagcgcag cgacgagatg
     7081 cgcggcgggc tccaggcgat gctcgacgcc ggcgagacct acatggacgg catcggcagc
     7141 gacgtggccg atcccgaggt caccgagtcg gcctacgcca aggtgccgct cgctccgctc
     7201 gtgtcgatgc tcgacggcgt cgccgacctg caggaccgcc tggccggcat caccgcgccg
     7261 gcgctgatca tgaccagccc cgaggaccac gtcgtggcgc ccacggcctc ggaccacctg
     7321 gccgccaccc tcggcgggtc ggtcgagcgg gtcaccctcg agcgcagcta ccacgtcgcc
     7381 accatcgacc acgaccgcga cctcatcatc gagcgcatcc gcgacttcgt cgcccgcgtc
     7441 accgcctgac ccgccctggg aggacgtggg cacctgctcg taggctcgct ccgtgcccgg
     7501 acacatcacc agcgacgacg tcgcccacgt ggcgcgcctg gcccgcctga cgttgaccga
     7561 cgacgagctc gacaccttca ccggccagct ggcgtcgatc ctcgaccacg ccgccgacgt
     7621 ggaggccctc gacctcgacg acgtcccgcc gacgtcgcac ccgtacccgc tcgagaacgt
     7681 ggtgcgcccc gacgaggtgc ggccgtgcct ggaccgcgac gaggtcctcg ccgaggcccc
     7741 atccgccgag gacggcatgt tccgggtccc gcccgtgctg ggggaggccc cgtgagcccg
     7801 gtcgaccgct tccagaccgc cgccggcctc gccgcggccg tcgccacggg ggagcgcagc
     7861 gcccgcagcg tcctcgacga gcacctcgcc cgcgtcgacg agcgcgaggg cgagatccac
     7921 gcgttcaacc acctgatggc cgacgaggcc cgggcccggg ccgacgacat cgacgcccgc
     7981 atcgcggcgg gcgaggacgt cggtccgctg gccggcgtgc cggtggcgct caaggacaac
     8041 ctctgcaccc ggggcatccc caccacctgc tcgtcgaaga tcctcgacga ctggcgtccg
     8101 ccctacgacg ccacggtcgt caaccgcctg cgtgacgccg gcgcggtcgt gatcggcaag
     8161 acgaacctgg acgagttcgc catgggctcc tccacggaga actccgcgtt cggccccacc
     8221 cgcaacccgc tcgacaccag ccgggtgccc ggtggttcct cgggtggctc ggccgctgcg
     8281 gtcgccgccg gcttcgcgcc gatctccctc ggctccgaca ccggcggctc catccgccag
     8341 cccgcggccc tgtgcggcgt ggtcggcgcc aagcccacct acggccgggc ctcccgctac
     8401 ggcctcatcg cgttcgcctc cagcctcgac cagatcggcc cgttcgccca cacggtcgag
     8461 gacgccgccc tgctcatgga ggtcgtcggc ggccacgacc cgctcgacgc caccagcatc
     8521 cccgagccct tccccgacct gcgcagcac
//
LOCUS       DATISU010000121         8050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_5783, whole genome shotgun sequence.
ACCESSION   DATISU010000121 DATISU010000000
VERSION     DATISU010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5783"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VLR27_05090"
     CDS             complement(<1..486)
                     /locus_tag="VLR27_05090"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSP02852.1"
                     /translation="MSELEAFPEDWDRALAVVAHPDDMEYGAASAVARWTGQGKWVGY
                     VLVTEGEAGISTMAPEEVAPIRRDEQIASCAVVGVTDVEFLDHPDGTIVEGLELRADL
                     AAAIRRHRPDVVLSINHHESWGGPSWNHADHRAVGRALLDAVRDAANPWVFPDRGEAW
                     DG"
     gene            complement(497..2116)
                     /locus_tag="VLR27_05095"
     CDS             complement(497..2116)
                     /locus_tag="VLR27_05095"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HSP02853.1"
                     /translation="MLNDHGGELALSVLRNAGVDTIYTLSGGHIFPFYDAAVKREPNV
                     RILDVRHEQTATFAAEAAAKLTRNVGVAVLTAGPGVTNGISAITGAQFNGSPVLVLGG
                     RAPQERWGSGSLQEFDHVPLLTPITKHAATVTSTDRIAADVSQAVETALTPHRGPVFL
                     DFPLDVVFGMAEVEVPGAVQPSGPAPDPDAVRRAAERIATAKTPAIVAGSDVWWGGAH
                     EALVAAVEALRVPVFLNGLGRGTVPADHELCFSRTRSLLKAEADVVVVIGTPLDFRLG
                     FGSFGDAEVVHVVDHPSLEPSHVEPAVRVSGDLATVLQGWADHAGDRTDHEPWIARLT
                     DAEAAAREADAPLLAAGDDPIKPTRIYGELAKRLDRDAVVVCDGGDFVSYAGKYVDVQ
                     TPGCWLDTGPFGCLGTGMGGAIAARVTHPDRQVVVLLGDGAAGFSLMDVDTLVRHDLP
                     VVMVVGNNGIWGLEKHPMQMVYGYDVAADLQPGTRYDDVVKALGGAGETVTDPDEIGP
                     AMDRAFASGVPYLVNVLTDPGDAYPRSSNLA"
     gene            2175..2705
                     /locus_tag="VLR27_05100"
     CDS             2175..2705
                     /locus_tag="VLR27_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSP02854.1"
                     /translation="MTEPGGAIVPDDKDWTWVLDRPCPECGFDTRSIDVTAVGDLIRA
                     NASAWQRVLAAPGAGHRPSPDRWSPLEYACHVRDVFRLFGERLELMLTEDGPHYANWD
                     QDATAVEDDYAGQSPVAVAVELTRAGSALADRFDAVEGDQWQRTGYRSDGAEFTVDTF
                     ARYFIHDPVHHLWDVS"
     gene            2709..3191
                     /locus_tag="VLR27_05105"
     CDS             2709..3191
                     /locus_tag="VLR27_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP02855.1"
                     /translation="MEIRPARPEEFAALGQLTLEAYRTVGPQSATYEAELVDVAHRAE
                     HATVLVAADDDGTLLGCLTLVLPGPNVLSEHREPEAASIRMLGVAPSARGRGIGEALT
                     LAAMAEARAVGAKTMVLHSATDMTAAHRLYRRLGFTRDESLDWVPEPDVELLGFRRAL
                     "
     gene            complement(3193..4794)
                     /locus_tag="VLR27_05110"
     CDS             complement(3193..4794)
                     /locus_tag="VLR27_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HSP02856.1"
                     /translation="MDAPAIPLPEPEIAPEIHARRWLILAVLCLSLIIVIIGNTSLNV
                     ALPTLAAELDASTSQLQWMVDAYALVFAGLLFTAGTLGDRWGRKGALQAGLLVFLLGA
                     AFASTADTAQAVIASRAVMGVAAAFVMPSTLSILTNVFSAEERPKAIAIWAGISGGGA
                     ALGPIASGWLLEHFFWGSVFLVNVPFILAALIAGALLVPSSKDPAESPLDVPGALLSI
                     IGLGALVYAIIEAPHYGWLSGQTLLTFGVALVAIVAFGARERTARFPMLDLSLFKDRR
                     FSVASAGITLTFFAMFGTFFLMAQYLQMVLGYTPFESGLFQLPFAFIMMLVAPRVPKL
                     VARWGAAKIVPVGLTFTAGGLAAFSSIGVDSSLLHVYASVLPLGLGMAITMTPLTTLI
                     MSSVPLGRAGVGSAMNDTTRELGGALGVAVLGSVVTSQYTSGIGASLVGLPDAARGMV
                     DSGLAGALAVAGQVGGAQGDALALAAKQAFVDGLNLAALVGSVVVFVAAFVSWKLLPR
                     TTPAPFVHPPRDDDPGLAEPSTLVD"
     gene            4948..5526
                     /locus_tag="VLR27_05115"
     CDS             4948..5526
                     /locus_tag="VLR27_05115"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP02857.1"
                     /translation="MARPRSEEARLRTIEATIQTLLEHGAEGLTMEEIADRSGVAKST
                     IYRHFGTREHLVTESVRSCVVEQSTPDSGSLADDLTDLFTRYDESEETKRLNELIPLL
                     IDAARRDAAMREVLDALLVERQRPLRTVLKLAQGRGEIDPDLDLDVAVAVLIGPFTYR
                     RMVQDAEITDEFVAAVVQTGIAALRSTAPGTD"
     gene            5654..6397
                     /locus_tag="VLR27_05120"
     CDS             5654..6397
                     /locus_tag="VLR27_05120"
                     /inference="COORDINATES: protein motif:HMM:NF019633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HSP02858.1"
                     /translation="MHLCEVVRTRPVTPMVQRVTLGGCGLAGFPAVGADAFAYLLLPP
                     AGRSELTIGLDFDWAQVRSMPRGERARGAYYSVRHHRPELLEIDLDVVLHDHGVASGW
                     AATAAPGDKAAVWGPRVLFRPPTDTAWTLLLADDTAVPAMLSILDHRPVDHRVVAVAE
                     VADAAEVRDVRPGPCVDLRWVRRDRGERTADVLRSLRLPPGPGYAWGGGEHAWIQELE
                     AMVTDVHGVDADHRSLTSYWRRNQPTGLR"
     gene            6394..7176
                     /locus_tag="VLR27_05125"
     CDS             6394..7176
                     /locus_tag="VLR27_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP02859.1"
                     /translation="MSTLTTHGLCVGHGDRTVVAGAELTFSPGRCTAIVGANGSGKST
                     LLRTVAGLAAPLGGEIRLGDRDLAALRARELARQLAFLPQAPLVPAGITVAELVALGR
                     HPHRRLLGGPTPGDDEAIAEAMAQTGLEDLAERPVDQLSGGERQRVWIAVALAQQAPV
                     LLLDEPTTFLDIRHQLEVLALARQLCDEGGRTVVAVLHDLNQAAAFADQLVVVGDGRI
                     LAQGPPGDVLSAELLARAFGVHAEVHLDDEARPTCRFHAALA"
     gene            7217..>8050
                     /locus_tag="VLR27_05130"
     CDS             7217..>8050
                     /locus_tag="VLR27_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSP02860.1"
                     /translation="MRRITFLFTLLALAAGLTACGDDDSGAAVSGDAAASAETEETTE
                     ESAGPITITDGTGTEVTLDEPAMTVVALEWALAESVVLLDAELAGAADAAGYGEWVAE
                     PALPEGVEDVGTRQEASIERIQALQPDLILGVDFRHAAVRDQLEAIAPTYLASPYAAD
                     AGSQYEDMRQTFEDIGALTGTEDRAAEVLDDLDAHLADAEAELEQAGLAGQEIGIAQG
                     FTTDGTPTVRMFSPNALVIELLAEIGLPNGWPGGDTEYGFDTVDGEGLTQLGDVELFT
                     IA"
BASE COUNT         1160 a         2914 c         2956 g         1020 t
ORIGIN      
        1 gccgtcccag gcctcccccc ggtcggggaa cacccacggg ttggcggcgt cgcgcacggc
       61 gtcgagcagg gcccggccca cggcgcggtg gtcggcgtgg ttccaggacg ggccgcccca
      121 ggactcgtgg tggttgatcg agagcaccac gtcgggccgg tgccggcgga tggcggcggc
      181 gaggtcggcc cgcagctcga ggccctcgac gatggtgccg tcggggtggt cgaggaactc
      241 cacgtccgtg acgccgacga cggcgcagct ggcgatctgc tcgtcccgcc ggatcggagc
      301 cacctcctcg ggcgccatgg tggagatgcc ggcctcgccc tcggtgacca gcacgtagcc
      361 gacccacttg ccctgcccgg tccagcgggc cacggcggac gccgctccgt actccatgtc
      421 gtcggggtgg gcgaccacgg ccagggcccg gtcccagtcc tcggggaatg cttcgagctc
      481 gctcacgatc tgctcctcac gccaggttgg acgagcgcgg gtaggcgtcg cccgggtcgg
      541 tcagcacgtt gacgaggtag ggcacgccgg aggcgaaggc gcggtccatc gccgggccga
      601 tctcgtcagg gtcggtgacg gtctcgccgg cgccaccgag ggccttgacg acgtcgtcgt
      661 agcgggtgcc gggctggagg tcggccgcca cgtcgtagcc gtagaccatc tgcatcgggt
      721 gcttctccag gccccagatg ccgttgttgc cgaccaccat cacgacgggg aggtcgtggc
      781 ggacgagggt gtccacgtcc atcagcgaga agccggcggc gccgtcgccg agcagcacca
      841 ccacctggcg gtcggggtgg gtgacccggg cggcgatggc gccgcccatg ccggtgccga
      901 ggcagccgaa cgggccggtg tcgagccagc accccggcgt ctgcacgtcc acgtacttgc
      961 cggcgtagct gacgaagtcg ccgccgtcac acacgaccac ggcgtcgcgg tcgaggcgct
     1021 tggccagctc gccgtagatg cgggtcggct tgatcgggtc gtcgccggcg gcgagcaggg
     1081 gggcatccgc ctcccgggcc gcggcctcgg cgtcggtgag gcgggcgatc cacggctcgt
     1141 ggtcggtccg gtcgccggcg tggtccgccc agccctgcag cacggtggcc aggtcgccgc
     1201 tgacccgcac cgcgggctcg acgtgcgacg gctccagcga ggggtggtcg accacgtgga
     1261 cgacctcggc gtcgccgaag ctcccgaagc cgaggcggaa gtccagcggc gtgccgatga
     1321 ccacgaccac gtcggcttcg gccttcagca ggctccgggt gcggctgaag cacagctcgt
     1381 ggtcggcggg cacggtgccg cggccgagcc cgttgaggaa cacgggcacc cgcagcgcct
     1441 ccacggcggc gaccagggcc tcgtgggcgc caccccacca cacgtccgag ccggcgacga
     1501 tcgccggggt cttcgccgtg gcgatgcgct cggcggcacg ccggacggcg tcggggtccg
     1561 gggccggtcc cgacggctgc accgcgccgg ggacctcgac ctcggccatg ccgaacacga
     1621 cgtcgagggg gaagtcgagg aacaccggac cgcggtgcgg ggtgagggcg gtctccacgg
     1681 cctgggacac gtcggcggcg atgcggtcgg tggacgtgac ggtcgcggcg tgcttcgtga
     1741 tgggcgtcag cagcgggacg tggtcgaact cctgcagcga gccggagccc cagcgctcct
     1801 gcggagcccg gccgccgagc accagcaccg gcgagccgtt gaactgcgcg ccggtgatgg
     1861 cgctgatgcc gttggtgacg ccggggccgg cggtgagcac ggccacgccg acgttgcggg
     1921 tgagcttggc cgcggcctcc gccgcgaagg tggccgtctg ctcgtggcgc acgtcgagga
     1981 tccgcacgtt cggctcccgc ttcaccgcgg cgtcgtagaa cgggaagatg tgcccgcccg
     2041 acagggtgta gatggtgtcg acgcccgcgt tgcgcaggac ggacagggcc agctcgccgc
     2101 cgtggtcgtt cagcatccgg ccagcctggc aaagcgagcc cagggcccgc ggcgggtgcg
     2161 cgaacctgct cgccatgacc gaaccaggcg gtgccatcgt ccccgacgac aaggactgga
     2221 cgtgggtgct cgatcgcccg tgccccgagt gcgggttcga cacgcgctcg atcgacgtca
     2281 ccgcggtcgg cgacctcatc cgggccaacg cgtcggcgtg gcagcgggtg ctggccgcac
     2341 cgggcgccgg gcaccggccg tcgccggatc gctggtcccc gctggagtac gcgtgccacg
     2401 tgcgcgacgt gttccgcctg ttcggtgagc ggctcgagct gatgctgacc gaggacggcc
     2461 cgcactacgc caactgggac caggacgcga ccgcggtcga ggacgactac gccggccagt
     2521 cacccgtggc cgtggcggtg gagctcaccc gggccggctc cgcgctggcc gaccgcttcg
     2581 acgccgtcga gggcgaccag tggcagcgca ccggctaccg ctccgacggg gccgagttca
     2641 cggtcgacac cttcgcccgg tacttcatcc acgacccggt ccaccacctc tgggacgtga
     2701 gctgatcgat ggagatccgc cccgcccgac ccgaggagtt cgccgccctc gggcagctga
     2761 cgctcgaggc gtaccgcacc gtcggcccgc agtccgcaac gtacgaggcc gagctggtcg
     2821 acgtcgccca ccgggccgag cacgccaccg tgctggtcgc ggccgacgac gacggcacgc
     2881 tcctcggctg cctcaccctc gtgctgcccg gaccgaacgt gctctccgag caccgggagc
     2941 ccgaggccgc gtcgatccgc atgctcggcg tcgccccgtc ggcgcgcggt cggggcatcg
     3001 gggaggcgct gacgctggcc gccatggcgg aggcccgagc agtcggggcc aagacgatgg
     3061 tcctgcactc ggccacggac atgaccgccg cccaccgcct ctaccggcgg ctcgggttca
     3121 cgcgcgacga gtcgctcgac tgggtgcccg agcccgacgt ggagctgctc gggttccgcc
     3181 gggcgctgta gctcagtcga ccagggtcga cggctcggcc aggccgggat cgtcgtcccg
     3241 gggcgggtgc acgaacggtg ccggcgtggt gcggggtagc agcttccagg acacgaaggc
     3301 ggcgacgaac accacgaccg agccgaccag cgccgcgagg ttgaggccgt cgacgaaggc
     3361 ctgcttggcg gcgagggcga gggcatcgcc ctgggcgcca ccgacctggc cggccacggc
     3421 gagcgcgccg gcgaggccgc tgtcgaccat gccccgggcc gcgtcgggca ggccgacgag
     3481 ggaggcgccg atgccgctgg tgtactgcga cgtcacgacc gacccgagga cggccacgcc
     3541 gagggcgccg ccgagctcgc gggtggtgtc gttcatggcc gagccgacgc cggctcgccc
     3601 gagcggcacc gacgacatga tcagcgtggt gagcggggtc atggtgatgg ccatgccgag
     3661 gccgaggggc agcaccgagg cgtagacgtg cagcagcgag ctgtcgacgc cgatgctcga
     3721 gaacgcggcc aggccgccgg cggtgaaggt gagcccgacc ggcacgatct tggccgcacc
     3781 ccagcgggcc accagcttcg gcacccgggg cgccaccagc atcatgatga aggcgaaggg
     3841 gagctggaac aggccggact cgaacggggt gtagccgagc accatctgca ggtactgggc
     3901 catgaggaag aacgtgccga acatggcgaa gaacgtcagc gtgatgccgg ccgaggccac
     3961 gctgaagcgg cggtccttga acagcgacag gtcgagcatc gggaaccggg cggtgcgctc
     4021 ccgagcaccg aaggcgacga tggcgaccag ggcgacaccg aaggtgagca gcgtctggcc
     4081 cgacagccag ccgtagtggg gggcctcgat gatggcgtag acgagggcgc cgaggccgat
     4141 gatggacagc agcgcgccgg gcacgtcgag ggggctctcg gccgggtcct tcgagctcgg
     4201 caccagcaga gcgccggcga tgagggcggc gaggatgaac ggcacgttca ccaggaacac
     4261 cgaaccccag aagaagtgct cgagcagcca gcccgacgcg atggggccca gggccgcgcc
     4321 gccgccggag atgccggccc agatcgcgat ggccttgggg cgctcctcgg cggagaagac
     4381 gttggtgagg atggacagcg tcgagggcat gacgaacgcg gcggccacgc ccatgacggc
     4441 gcgggaggcg atgacggcct gggcggtgtc ggccgtggac gcgaacgccg ccccgagcag
     4501 gaacacgagc aggccagcct ggagggcacc cttgcgaccc cagcggtcgc cgagggtgcc
     4561 ggcggtgaag agcaggccgg cgaagaccag ggcgtaggcg tcgaccatcc actgcagctg
     4621 gctggtggag gcgtcgagct cggcggccag cgtgggcagc gccacgttca ggctcgtgtt
     4681 gccgatgatg acgatgatca ggctgaggca gagcaccgcg aggatcagcc agcggcgggc
     4741 gtggatctcg ggtgcgatct cgggctcggg gagggggatc gcaggggcgt ccacgggagg
     4801 tgctccgctc gtctttcgaa ggatcaggag agggggtctc cgcaccggac cagggggagg
     4861 ttccgatacg aagacgtttc gcaatggtaa cgccatcaac ctgggcgctg cacggtctat
     4921 tccgaggttt cgctacggtg gtgacccatg gcacggcccc gcagcgagga ggcccgcctc
     4981 cgcacgatcg aggcgaccat ccagaccctg ctcgagcacg gcgccgaggg cctcaccatg
     5041 gaggagatcg ccgatcgctc cggcgtggcc aagtccacga tctaccgcca cttcggcacc
     5101 cgggagcacc tggtcaccga gtcggtgcgc tcctgcgtgg tcgagcagtc cacgcccgac
     5161 tcgggctcgc tggccgacga cctcaccgac ctgttcaccc gctacgacga gtcggaggag
     5221 accaagcggc tcaacgagct gatcccgctg ctcatcgacg ccgcccgccg cgacgccgcc
     5281 atgcgcgagg tcctcgacgc gctgctcgtg gagcgccagc gccccctgcg caccgtgctg
     5341 aagctggccc agggccgggg cgagatcgac cccgacctcg acctcgacgt ggccgtcgcc
     5401 gtgctcatcg ggcccttcac ctaccggcgc atggtccagg acgccgagat caccgacgag
     5461 ttcgtcgccg ccgtggtcca gaccggcatc gccgccctgc gctccaccgc acccgggacg
     5521 gactgaccac ccgccagcgc ctcgcgcaac acctgcactg ccagttcgcc tccaatggaa
     5581 ctggcagtgc aggtgttgcg ctgcttagat gttcggcatg cctaacagca gagatgcaga
     5641 caccttgcca cgggtgcacc tctgcgaggt cgtgcgcacc cggccggtca cgccgatggt
     5701 gcagcgggtc acgctcggcg gctgcggcct cgccggcttc cccgccgtgg gcgccgatgc
     5761 gttcgcctac ctgctcctgc cgccggcggg ccgcagtgag ctgaccatcg gactggactt
     5821 cgactgggcc caggtccggt cgatgccgcg aggcgagcga gcgcgaggcg cctactacag
     5881 cgtccgccac caccggcccg agctgctcga gatcgacctc gacgtggtgc tgcacgacca
     5941 cggggtcgcg tcgggttggg cggcgaccgc tgcgccgggt gacaaggccg cggtgtgggg
     6001 tccccgggtc ctgttccgcc ctccgaccga cacggcgtgg acgctgctgc tcgcggacga
     6061 caccgcggtc ccggcgatgc tctcgatcct cgaccaccgg ccggtcgatc accgggtggt
     6121 ggcggtcgcc gaggtggccg acgccgccga ggtgcgcgac gtccggcccg gaccgtgcgt
     6181 cgacctgcgc tgggtccgcc gggaccgggg cgaacggacg gccgacgtgc tgcgctccct
     6241 ccgtctgccg cccgggcccg gctacgcgtg gggtggcggc gagcacgcct ggatccagga
     6301 gctcgaggcg atggtgaccg atgtccacgg cgtcgacgcc gatcaccgct cgctcacgtc
     6361 gtactggcgc cggaaccagc cgaccggcct gcgatgagca cgctcaccac ccatgggctg
     6421 tgcgtgggcc acggcgaccg cacggtcgtg gccggcgccg agctgacctt ctcccccggt
     6481 cggtgcaccg cgatcgtcgg cgccaacggc tcggggaagt cgaccctgct gcgcaccgtg
     6541 gccggcctgg ccgcaccgct cggcggcgag atccggctgg gcgaccggga cctcgccgcg
     6601 ctccgggccc gggagctggc ccgccagctg gcgttcctgc cccaggcccc gctggtgccg
     6661 gccggcatca ccgtggccga gctggtcgcg ctcggacgcc acccgcatcg ccgcctgctc
     6721 ggcgggccga ccccgggcga cgacgaagcc atcgccgagg ccatggccca gaccggcctg
     6781 gaggacctcg ccgagcgacc ggtcgaccag ctctccggcg gcgagcgcca gcgggtgtgg
     6841 atcgccgtcg ccctcgccca gcaggcgccg gtactgctgc tcgacgagcc gaccaccttc
     6901 ctcgacatcc gccaccagct cgaggtgctc gccctggccc gccagctctg cgacgaaggg
     6961 gggcgcaccg tcgtggccgt cctccacgac ctcaaccagg ccgccgcgtt cgccgaccag
     7021 ctcgtggtcg tcggcgacgg tcggatcctg gcccagggcc cgcccggcga cgtgctgtcc
     7081 gccgagctgc tggcccgggc cttcggcgtg cacgccgagg tgcacctcga cgacgaggcc
     7141 cgtcccacct gccggttcca cgccgccctc gcctgacctc atcccccatc aaacgaaccc
     7201 ccctgaggag accccgatgc gacgcatcac gttcctgttc accctgctgg ccctcgccgc
     7261 cggcctgacc gcctgcggcg acgacgacag cggcgcggcg gtgtccggtg acgccgcggc
     7321 gagcgccgag accgaggaga ccaccgagga gtccgccggc ccgatcacca tcaccgacgg
     7381 cacgggcacc gaggtcaccc tcgacgagcc ggccatgacc gtcgtcgccc tcgagtgggc
     7441 actggccgag agcgtcgtgc tgctcgacgc cgagctcgcc ggggccgccg acgccgccgg
     7501 ctacggcgag tgggtggccg agccggcgct gcccgagggc gtcgaggacg tcggtactcg
     7561 ccaggaggcc tcgatcgagc gcatccaagc cctgcagccc gacctcatcc tgggcgtcga
     7621 cttccgccac gccgcagtgc gcgaccagct cgaggccatc gccccgacct acctcgccag
     7681 cccgtacgcc gcggacgccg gcagccagta cgaggacatg cgccagacgt tcgaggacat
     7741 cggcgccctc accggcaccg aggaccgggc ggccgaggtg ctcgacgacc tcgacgccca
     7801 cctcgccgac gccgaagccg agctggagca agccggcctg gccgggcagg agatcggcat
     7861 cgcccagggg ttcaccaccg acggcacccc gacggtgcgg atgttctccc ccaacgccct
     7921 cgtgatcgag ctgctcgccg agatcggcct ccccaacggc tggcccggcg gcgacaccga
     7981 gtacggcttc gacaccgtcg acggcgaggg cctgacccag ctgggtgacg tggagctgtt
     8041 cacgatcgcc
//
LOCUS       DATISU010000122         3764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_797266, whole genome shotgun sequence.
ACCESSION   DATISU010000122 DATISU010000000
VERSION     DATISU010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797266"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..582)
                     /locus_tag="VLR27_05135"
     CDS             complement(217..582)
                     /locus_tag="VLR27_05135"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HSP02861.1"
                     /translation="MDRDDDELDELQVALSARPPSELSALLDRVVRGMGAPGTDAIEL
                     VFNRWSDVVGGVLATKTRPISLERATLVLQADDPAVVSHVKWLEQDLLGRLDELLGPG
                     RVTAVEVRVARPGRQSRRP"
     gene            complement(627..1712)
                     /locus_tag="VLR27_05140"
     CDS             complement(627..1712)
                     /locus_tag="VLR27_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HSP02862.1"
                     /translation="MALHLQHLWLTDYRSYESADVTLPGGTTAIIGRNGQGKTNLLEA
                     VAYLAGSSLRGATTEAMVRTGAERAVVRAEAVRGPRELLVEAEIAARGRSRMQLNRQP
                     VKRRRDLLDALSVTLFSPDDLVLVKGGPGERRRWLDEAAVGLDHQTEGVRSDVERILK
                     QRNALLKQSRGRLDEAAALTLDVWDDRLTRAGEALAERRAAALAELVPRMTEAYRDLA
                     GIPLPVSATYDAPWRSTGLAAALADARDQDLRRGVTTVGPHRDEVVFGIDDLPARTHA
                     SQGEQRSLALAMRLAVHRAITETSGESPILLLDDVLSELDGGRTEALLAHLPPGQTLL
                     TTAGPLPQQTEPELVLRVADGRIAPDA"
     gene            complement(1717..2820)
                     /gene="dnaN"
                     /locus_tag="VLR27_05145"
     CDS             complement(1717..2820)
                     /gene="dnaN"
                     /locus_tag="VLR27_05145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HSP02863.1"
                     /translation="MKLRCERDVLAEALATAGRAVASRGALPVLSGVRLELAGDRLTI
                     TGSDLELTVTVELSVAGLADGVAVVPSRLVNDIVKALEPGAVELETGDDDARIASGRS
                     EFSLKTLPADEFPRVAAVAGDEVRFDADALTSALRQVLPAASGDDNRPILTGVLLAAE
                     DNGLRLVATDSYRLAVRDLPGVSVLSSGKQVLVPSRALAELSRALGDGGELTLRLGER
                     DASFATANDSGGIVLTTRLIEGEYPNYQGLIPQNQPNLLTVPREPLIEALRRVKLLAR
                     DATPVRLIMSADGLELKAVTQDVGEGSESLDATYQGEDLTVAFNPDYLLAGIEAASGE
                     EITLSTMDSLKPAVVRTPASDDYLYLLMPVRVQ"
     gene            complement(3224..>3764)
                     /locus_tag="VLR27_05150"
     CDS             complement(3224..>3764)
                     /locus_tag="VLR27_05150"
                     /inference="COORDINATES: protein motif:HMM:NF019903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP02864.1"
                     /translation="DIQPPDVETRLAILRKKAEDEASPVPDEILAFIAENINDNNIRA
                     LEGALTRLTAWASLNREELTTDKATEILADLVAEREARPITPETILRATAEYFGYTVE
                     ELIGRSRTRPLTTARHVGMYLFRELTDLSFPNIAKEFGGRDHTTVIHAVDRIKTQMKE
                     RRQVFQQVTELIQRLKAEG"
BASE COUNT          482 a         1273 c         1432 g          577 t
ORIGIN      
        1 gacgacctcc cacacgcagt ggtgcaggcc gttcgggccg gtggacccga tgtacatgcc
       61 gggacgcttc cggacggcct cgaggccctc caggacccgc atgttgttgg cgccgtacga
      121 cgtcgtctca gcagccaaaa ccactcctca tctaccaaat gacagtggtg gcatcctacc
      181 agcgggggtc cgacatcccg gccatctggg gcggcctcac gggcgtcgcg actggcgtcc
      241 cgggcgggcg acgcggacct ccaccgcggt gacccgaccc ggtcccagca gctcgtcgag
      301 gcggccgagc agatcctgct ccagccactt cacgtggctc accacggccg ggtcgtcggc
      361 ctgcaggacc agggtggcgc gctcgaggga gattggccgg gtcttggtgg ccaggacgcc
      421 gccgacgacg tcggaccacc ggttgaacac cagctcgatg gcgtcggtgc ccggcgctcc
      481 catcccccgc acgacgcggt cgagcagggc ggacagctcg ctcggcggcc gggcggacag
      541 cgccacctgc agctcgtcga gctcgtcgtc gtcgcggtcc accagccgag tgtggcactc
      601 ccccacggac cgcaccccgg cccgggtcag gcgtcgggtg cgatgcgccc gtcggccacc
      661 cgcagcacca gctcgggctc ggtctgctgg ggcagcggac cggccgtggt gagcagcgtc
      721 tggccggggg gcagatgggc gagcagggcc tcggtgcgcc cgccgtcgag ctcggacagc
      781 acgtcgtcga gcagcaggat cgggctctcg ccgctggtct cggtgatggc ccggtggacg
      841 gcgaggcgca tggcgagggc gagggagcgc tgctcgccct ggctggcgtg ggtgcgagcc
      901 gggaggtcgt cgatgccgaa cacgacctcg tcccgatggg ggccgaccgt ggtgacgcca
      961 cgacgcaggt cctggtcccg ggcgtcggcg agggccgcgg cgagcccggt cgagcgccag
     1021 ggggcgtcgt aggtggccga cacgggcagg gggatgccgg cgaggtcccg gtacgcctcg
     1081 gtcattcggg gcacgagctc agccagcgcg gcggcccgcc gctcggccag ggcctccccg
     1141 gcccgggtca gtcggtcgtc ccacacgtcg agggtgagcg cggcggcctc gtcgaggcga
     1201 cctcggctct gcttgagcag ggcgttgcgc tgcttgagga tccgctcgac gtcgctgcgg
     1261 accccttcgg tctggtggtc gagcccgacc gcggcctcgt ccagccaccg gcgacgctcg
     1321 ccggggccgc ccttgaccag caccaggtcg tcggggctga acagcgtcac cgagagcgcg
     1381 tcgagcaggt cccgccggcg cttcaccggc tggcggttca gctgcatccg gctccggccg
     1441 cgagcggcga tctcggcctc gacgagcagc tcgcgggggc cgcgcaccgc ctcggcccgc
     1501 acgacggccc gctcggcacc ggtgcgcacc atggcctcgg tggtggcacc gcgcagcgag
     1561 gagccggcca ggtacgccac ggcctcgagc aggttggtct tgccctggcc gttgcggccg
     1621 atgatggccg tggtgccgcc cgggagcgtg acgtcagccg actcgtagct gcggtagtcg
     1681 gtgagccaga ggtgctggag gtggagggcc aggggcctat tgcacccgca ccggcatcag
     1741 caggtacagg tagtcgtccg aggcgggtgt gcggaccacg gcgggcttga gcgagtccat
     1801 ggtggacagg gtgatctcct cacccgacgc ggcttcgatc ccggccagca ggtagtcggg
     1861 gttgaacgcc accgtgaggt cctcgccctg gtatgtggcg tccaggctct cggagccctc
     1921 gccgacgtcc tgggtcaccg ccttcagctc gaggccgtcg gccgacatga tcaagcggac
     1981 cggtgtggcg tcgcgggcca gcagcttcac gcggcgcagc gcctcgatga gcggctcgcg
     2041 gggcacggtg agcaggttcg gctggttctg cgggatgagg ccctggtagt tcgggtactc
     2101 gccctcgatg aggcgggtgg tgaggacgat gcccccgctg tcgttcgcgg tggcgaagct
     2161 ggcgtcgcgc tcgcccaggc gcagcgtgag ctcgcctccg tcgccgaggg cccggctgag
     2221 ctcggccagg gcccgggagg gcaccagcac ctgcttgccc gacgacagca cgctgacgcc
     2281 ggggaggtcg cgcaccgcga gtcggtagga gtccgtggcc accagacgca ggccgttgtc
     2341 ctcggcagcg aggagcacac cggtgaggat cgggcggttg tcgtccccgc tggcggcggg
     2401 cagcacctgg cgcagcgcgc tggtgagcgc gtcggcgtcg aagcgcacct cgtcgccggc
     2461 gacggccgcg acccgcggga actcgtcggc cggcagggtc ttgagggaga actccgagcg
     2521 cccagacgcg atgcgggcat cgtcgtcacc ggtctcgagc tccaccgcgc cgggctcgag
     2581 ggctttgacg atgtcgttca ccagtcggct gggcaccacg gcgacgccgt cggccaggcc
     2641 ggcgacggac agctcgacgg tgaccgtgag ctccagatcg gagccggtga tggtcagtcg
     2701 atcccccgcc agctcgaggc ggaccccgga cagcacgggc agagcaccac ggctggcgac
     2761 cgcccgcccg gccgtggcca gggcctcggc gagcacgtct cgttcacatc gcagcttcat
     2821 gaggggtgtg cgctccttgc atcgcctgct gctgctcggt cgctccgagc gggttgtccc
     2881 gtcttcccac ggtgatgttg tacggatgtc gtgggaggtg gtgggggttg tggattgtgg
     2941 atactgcccc atccgggcag gtcagcgggg tgatcgtcgt cccagcgatg tccccagtgg
     3001 tcccctgctg gtcgtgcgcc ggacaccgtg acgtcaccgg ggtccccatc tcggggggtg
     3061 tgcccacacg gcgtgcacag gcgcgtccac cgatcgtggc gtctgcctgt ggatgcggtg
     3121 ctcgacccgg cggtcggcgt ccccatggca tcccacgcgc gtccccgtgg cgtccccacg
     3181 accgaccggc ccgggtccac acgttgtcca cagggtggga gggtcatccc tcggccttga
     3241 ggcgctggat cagctcggtg acctgctgga acacctggcg gcgctccttc atctgggtct
     3301 tgatgcgatc gacggcgtgg atgacggtgg tgtggtcccg tccgccgaac tccttggcga
     3361 tgttcgggaa gctcaggtcg gtgagctcgc ggaacaggta catgccgacg tggcgggccg
     3421 tggtgagcgg acgggtgcga gaccggccga tgagctcctc gaccgtgtag ccgaagtact
     3481 ccgccgtggc ccgcaggatc gtctcggggg tgatgggccg ggcctcgcgc tcggccacca
     3541 ggtcggcgag gatctcggtg gccttgtcgg tggtgagctc ctcgcggttg agcgacgccc
     3601 aggcggtgag gcgggtgagg gcaccctcga gggcccggat gttgttgtcg ttgatgttct
     3661 cggcgatgaa ggcgaggatc tcgtcgggga ccggggaggc ctcgtcctcg gccttcttgc
     3721 gcaggatggc caggcgggtc tcgacgtcgg gcggctggat gtcg
//
LOCUS       DATISU010000123         5751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_844459, whole genome shotgun sequence.
ACCESSION   DATISU010000123 DATISU010000000
VERSION     DATISU010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844459"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VLR27_05155"
     CDS             <1..999
                     /locus_tag="VLR27_05155"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HSP02865.1"
                     /translation="DLVLPGHLRPRYRVVGEGRTLAEGDDLRELERRLRAHVRATVVS
                     SAPELERSGLTSWDFGTLPRVVESTVDGFTVQGYPAVIDTQDAVGVRILASVDQQRAL
                     HWNGVRRLLALQLGKPARVLQKRLDARVLLGLTAAPHGTVTAAVDDALHGVLDALLLD
                     AGGPPFDEDGYAALRRRVQDRWLDDLVVVVTTLSGVVQHAERLRERLAQPAPTSWSAS
                     LDDVTHQLTRLVFDGMVVSIGTDRLAHLPRYLDAIDARLDKLREGPAKDVERLRTIAR
                     LEAEHAELVSVHGLTAALDDVRWLIEELRVQLFAQQLGTAVSVSEKRIRERLQRLR"
     gene            complement(992..1849)
                     /locus_tag="VLR27_05160"
     CDS             complement(992..1849)
                     /locus_tag="VLR27_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HSP02866.1"
                     /translation="MDDALLDAIASALGARPVSLAAVSGGDVAAASRAELDDGRIVFV
                     KTHHDPPPGFFTTEATGLRWLRDVGSVLVPEVLAVSDDPPFLALEWIDEGRAGTDTEA
                     DLGRGLAELHLAGAPSFGRQDRRTTGSRGLPNEPCETWAEFYATQRLLPLVRLARDAG
                     ALPDTAIADLERVAARLDDLGGPAEPPARLHGDLWAGNRLVDRDGRSWLIDPAAHGGH
                     REFDLAMMRLFGGFGAACFATYDDVHPLADGWEARVPLHQLAPLVVHAIKFGGGYVAG
                     TERALAQLT"
     gene            1911..3047
                     /locus_tag="VLR27_05165"
     CDS             1911..3047
                     /locus_tag="VLR27_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HSP02867.1"
                     /translation="MLDLTVASIPIYFGTMAAEHRYLRTRAAEQGPSPADYTPEDTAT
                     SLAMGLGSLLVPLVLPKVVRHVVPGKGRYGKVLIGVAAAAAVATTVADRLADGDEVEP
                     ARSTTRRRLGRIARRARRVTGPVAVGAGALAVTTSFAARTNAEAQWAAGGAERDKGNG
                     PLPWFVAIAGWDFLYYWNHRFMHEVRALWAIHVVHHSSERYNLSTALRQPVADVLGLW
                     LPYGVIARVGIRPHLLAQARALNLLYQYWVHTETIRTLGPAEEVLNTPSHHRVHHGSQ
                     RQYLDRNHGSILIVWDRLFGTFEREDERVVYGLTTNIDTYNPVKVATHEYRDIVRDVA
                     GSTTWRDRLGFVLRGPGWAYERHRQLAAEGEGIVADPDEAVTAA"
     gene            complement(3105..3317)
                     /locus_tag="VLR27_05170"
     CDS             complement(3105..3317)
                     /locus_tag="VLR27_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HSP02868.1"
                     /translation="MIEHESEETMSREAAADRLREIADQLSRHNEIAFDLDGLKTSVK
                     VPNEVTLSVEVEVGDDGNEIEIEISW"
     gene            complement(3335..4300)
                     /locus_tag="VLR27_05175"
     CDS             complement(3335..4300)
                     /locus_tag="VLR27_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSP02869.1"
                     /translation="MAAEGGRTTAAVVLGSGGARGYAHIGALQVILERGFDIVAIAGS
                     SMGALVGGVHAAGCLDEYAAWVTGLRQLDVLRLVDLSLSAGGAIRGEKVFAIVREMVG
                     DMQIQDLPIAFTAVATDLLAHREVWFQEGSLSNAIRASIAIPSLFTPVVSGDQLLVDG
                     ALMNPVPILPTLSANADITIAVHLSGEVRRHSPTRPSAPLTTPVLEDDSVGGPVDAPG
                     LRQRAARFFDWEAVRAMLSRDEPGERPPPPGVDIADVGRLDIVNLAYEVMQATLTSYK
                     LAGYPPDVLVAIPKASARTFEFHRAPELIELGRERTEQALTDAGL"
     gene            complement(4290..4754)
                     /locus_tag="VLR27_05180"
     CDS             complement(4290..4754)
                     /locus_tag="VLR27_05180"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HSP02870.1"
                     /translation="MSELAERMQQDLTAAMKARDEVRVATLRMAISAVREEAVAGKQA
                     RELSDDDVEKVLRTQVKRRDEAAEAFQAAGRDEQAQRELDERSILSAYLPEELGDDEL
                     EALVAEALASGGFTEPSQMGQAMNATMAAVAGRAEGKRVSAMVKAKLAGNGG"
     gene            4923..5204
                     /locus_tag="VLR27_05185"
     CDS             4923..5204
                     /locus_tag="VLR27_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02871.1"
                     /translation="MAALRQPVEPARLRATIAGRPVLEEAAVVLLTDRSILVEVRDAV
                     TALAFSVAPEVVAIVDFGGSTITWSTEPSRRADDAPASRQLELVLRERH"
     gene            5252..>5751
                     /locus_tag="VLR27_05190"
     CDS             5252..>5751
                     /locus_tag="VLR27_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSP02872.1"
                     /translation="MADATSSDPSSYDELGLFHENADEAGLDWHGPPTVRRVETDLGD
                     GRTLSGLRWGTDAPQVVLLHGGGQNAHTWDTVALALGRPLLALDLPGHGHSGDVDPTR
                     TVGPRSFAEDVAIAVREHAPNAAAVVGMSLGGLTALELTVVAPELVRRLVMVDVTPGA
                     DREKAS"
BASE COUNT          748 a         2166 c         2066 g          771 t
ORIGIN      
        1 gacctcgtgc tgcccgggca cctccgcccc cgctaccgcg tggtgggcga gggccggacc
       61 ctggccgagg gcgacgacct gcgggagctg gagcgccggc tccgggccca cgtgcgcgcc
      121 accgtggtgt ccagcgcgcc ggagctcgag cgcagcggcc tcacgtcctg ggacttcggg
      181 accctgccgc gggtggtgga gtccaccgtc gacgggttca ccgtgcaggg ctacccagcc
      241 gtcatcgaca cccaggacgc cgtcggcgtg cggatcctcg cctccgtcga ccagcagcgc
      301 gccctgcact ggaacggcgt gcgccggctg ctcgccctgc agctggggaa gccggcccgg
      361 gtgctgcaga agcggctcga cgcccgggtg ctcctcggcc tcaccgccgc cccccacggc
      421 accgtcaccg ccgccgtcga cgacgcgctg cacggcgtcc tcgacgccct cctgctcgac
      481 gccggcggcc ccccgttcga cgaggacggc tacgccgccc tgcgccggcg ggtgcaggac
      541 cgctggctcg acgacctggt cgtcgtggtg acgacgctgt cgggcgtggt gcagcacgcc
      601 gagcgcctgc gcgagcggct ggcgcagccg gcgccgacgt cgtggtcggc ctcgctggac
      661 gacgtcaccc accagctcac ccggctggtg ttcgacggca tggtcgtcag catcggcacc
      721 gaccggctcg cccacctgcc ccgctacctg gatgcgatcg acgcccgcct cgacaagctg
      781 cgggagggcc cggccaagga cgtcgagcgg ctccggacca tcgcccgcct cgaggccgag
      841 cacgcagagc tggtgtcggt ccacgggctc accgccgcgc tggacgacgt gcgctggctg
      901 atcgaggagc tgcgggtgca gctcttcgcc cagcagctcg gcaccgcggt gtccgtgagc
      961 gagaagcgca tccgcgagcg cctccagcgc ctcaggtgag ctgggcgagg gcgcgctcgg
     1021 tgccggcgac gtagccgccg ccgaacttga tggcgtgcac gacgaggggc gccagctggt
     1081 ggagcggcac ccgggcctcc cagccgtcgg ccagcgggtg gacgtcgtcg taggtggcga
     1141 agcaggccgc accgaaccca ccgaacaggc gcatcatcgc caggtcgaac tcgcggtggc
     1201 cgccgtgtgc cgcggggtcg atcagccagc tgcgcccgtc ccggtcgacg aggcggttgc
     1261 cggcccacag gtcgccgtgc agccgggccg gtggctcggc cgggccgccg aggtcgtcga
     1321 gccgggcggc gacgcgctcg aggtcggcga tggctgtgtc cgggagggca cctgcgtcgc
     1381 gggccagccg gaccagcggc aggaggcgct gtgtggcgta gaactcggcc cacgtctcgc
     1441 agggctcgtt gggcagtcca cggctgccgg tggtgcgacg gtcctggcgc ccgaacgacg
     1501 gcgcacccgc gaggtggagc tcggccaggc cccggccgag gtcggcctcg gtgtccgtac
     1561 cggcccgacc ctcgtcgatc cactcgaggg ccaggaacgg cgggtcgtcc gacaccgcca
     1621 gcacctcggg caccagcacc gagccgacgt cgcgcagcca gcgcagcccg gtggcctcgg
     1681 tcgtgaagaa ccccggcggg gggtcgtggt gggtcttcac gaagacgatc cggccgtcgt
     1741 ccagctcggc ccgggaggca gcggcgacgt cccctcccga gacggcggcg agcgacacgg
     1801 gacgggcacc gagcgccgag gcgatcgcat cgaggagcgc gtcgtccatc cccggcgacc
     1861 gtaccgcccg gctcggtgcc gccctgtccg atctgtcacg atctccggtg atgttggacc
     1921 tcacggtcgc ctcgatcccc atctacttcg ggacgatggc ggcggagcac cgctacctgc
     1981 gcacgcgagc cgccgagcag ggccccagcc cggctgacta cacgcccgag gacaccgcca
     2041 catcgctggc gatgggactc ggctcgctcc tggtcccgct ggtgctgccc aaggtcgtgc
     2101 gccacgtcgt gcccggcaag ggccggtacg gcaaggtgct catcggcgtg gctgcggccg
     2161 cggccgtcgc caccacggtg gccgaccgcc tggccgacgg cgacgaggtc gagccggccc
     2221 ggtcgacgac gcgccggcgc ctcggccgca tcgcccggcg ggcccgtcgg gtcaccggcc
     2281 cggtggcggt cggcgccggt gcgctggcgg tcacgacgtc gttcgcggcc cggaccaacg
     2341 ccgaggcgca gtgggcggcc ggcggcgccg agcgcgacaa gggcaacggg ccgctgccgt
     2401 ggttcgtcgc catcgccggg tgggacttcc tctactactg gaaccaccgg ttcatgcacg
     2461 aggtccgggc gctgtgggcg atccacgtcg tccaccactc ctccgagcgc tacaacctgt
     2521 ccaccgcgct gcgccagccc gtcgccgacg tgctcggcct gtggctgccg tacggcgtga
     2581 tcgcccgcgt cggcatccgc ccgcacctgc tcgcccaggc ccgcgcactc aacctgctct
     2641 accagtactg ggtgcacacc gagacgatcc gcacgctcgg cccggccgag gaggtgctga
     2701 acacgccgtc ccaccaccgg gtgcaccacg gctcccagcg ccagtacctc gaccgcaacc
     2761 acggttcgat cctgatcgtc tgggaccgcc tgttcggcac cttcgagcgc gaggacgagc
     2821 gggtcgtcta cgggctcacg accaacatcg acacgtacaa ccccgtgaag gtcgccacgc
     2881 acgagtaccg cgacatcgtg cgcgacgtcg ccggctccac cacgtggcgg gaccgcctcg
     2941 gcttcgtcct gcgcggcccg gggtgggcct acgagcgcca ccgccagctg gccgccgagg
     3001 gcgagggcat cgtggccgat ccggacgagg ccgtcaccgc cgcctgaaat cccgtggatc
     3061 cgtcaccacc ggtggtacgg atccacggaa tttccgaggg gtggctacca ggagatctcg
     3121 atctcgatct cgttgccgtc gtcgccgacc tcgacctcga ccgagagggt cacctcgttg
     3181 ggcaccttga ccgacgtctt cagcccgtcg aggtcgaagg cgatctcgtt gtggcgggac
     3241 agctggtcgg cgatctcgcg cagcctgtcg gcggcggcct cccggctcat ggtctcctcg
     3301 gactcgtgct cgatcatgtc ggccagtctc gcagtcagag ccccgcgtcg gtgagcgcct
     3361 gctcggtgcg ctcccgaccg agctcgatga gctcgggggc gcggtggaac tcgaacgtgc
     3421 gggcgctggc cttggggatg gcgacgagca cgtcgggcgg gtagccagcg agcttgtagc
     3481 tggtgagggt ggcctgcatg acctcgtagg cgaggttgac gatgtcgagg cggccgacgt
     3541 cggcgatgtc gaccccgggc ggcggcgggc gctcgccggg ctcgtcgcgg ctgagcatcg
     3601 cccgcaccgc ctcccagtcg aagaaccggg cggcccgctg gcgcaggccc ggtgcgtcga
     3661 ccggcccgcc cacgctgtcg tcctcgagca ccggggtggt gaggggcgcg ctcggccggg
     3721 tgggggagtg gcggcggacc tcgccggaga ggtgcacagc gatcgtgatg tcggcgttgg
     3781 ccgacagcgt gggcaggatc ggcaccgggt tcatgagggc gccgtccacc aggagctggt
     3841 cgccggacac caccggcgtg aacagcgacg ggatggcgat ggacgcgcgg atggcgttgc
     3901 tgagcgatcc ctcctggaac cacacctcgc ggtgggcgag caggtcggtg gcgaccgccg
     3961 tgaacgcgat cggcaggtcc tggatctgca tgtcgcccac catctcgcgc acgatggcga
     4021 agaccttctc cccccggatc gctccgccgg cggacagcga caggtcgacc aggcgcagca
     4081 cgtcgagctg gcgcagcccg gtgacccacg ccgcgtactc gtcgaggcag ccggcggcgt
     4141 gcaccccacc gaccagggcg cccatcgacg agccggcgat ggcgacgatg tcgaagccgc
     4201 gctcgaggat gacctggagg gcgccgatgt gcgcgtagcc gcgggcaccg ccgctgccga
     4261 gcaccacggc cgcggtcgtg cgccctccct cagccgccat tgccggccag cttggccttc
     4321 accatcgccg agacccgctt gccctcggcc cggccggcga cggcggccat ggtcgcgttc
     4381 atcgcctgac ccatctgcga cggctcggtg aacccacccg aggcgagggc ctcggcgacg
     4441 agggcctcga gctcgtcgtc gcccagctcc tcggggaggt acgccgacag gatcgagcgc
     4501 tcgtccagct cgcgctgggc ctgctcgtcg cgcccggcgg cctggaacgc ctcggcggcc
     4561 tcgtcccgtc gcttcacctg ggtgcgcagc accttctcga cgtcgtcgtc gctgagctcg
     4621 cgggcctgct tccccgccac cgcctcctcg cggacggcgg agatggccat gcgcagggtg
     4681 gccacccgca cctcgtcgcg ggccttcatg gcggcggtca ggtcctgctg catgcgctcg
     4741 gcgagctcgc tcatcggtgg tcctccgggt cgggtgcccc gagcctgcct cacggcacct
     4801 cgatctgcca cggctccgac accagccacg gcggagggct gtcgtacgat cggctcgacg
     4861 acgatcggcg gagacccgtg gccttcgaag catccccgaa cccagaccgg cgctcgccac
     4921 ccatggccgc gctccgccag ccggtggagc ccgctcgcct gcgcgccacg atcgccgggc
     4981 gacccgtcct cgaggaggcc gcggtggtcc tgctcaccga tcgctcgatc ctggtcgagg
     5041 tgcgcgacgc ggtgaccgcc ctcgccttca gcgtggcacc cgaggtcgtc gccatcgtcg
     5101 acttcggcgg cagcaccatc acctggtcga ccgaaccgag ccggcgcgcc gacgacgccc
     5161 ccgcctcacg ccagctcgag ctggtgctgc gcgagcgcca ctgagctcct gcggcgccga
     5221 ggcccggcgt atccggtcct agagtccggc catggccgac gccacctcct ccgacccgag
     5281 cagctacgac gagctcggcc tgttccacga gaacgccgac gaggccggcc tggactggca
     5341 cgggccgccg accgtccggc gggtggagac cgacctcggg gacgggcgca ccctcagcgg
     5401 gttgcgctgg gggaccgacg caccccaggt cgtgctgctc cacggcggcg gccagaacgc
     5461 gcacacgtgg gacaccgtgg ccctggcgct cggccggccg ctgctcgccc tcgacctgcc
     5521 cggccacggg cacagcggcg acgtggaccc gacccggacg gtcgggcccc ggtccttcgc
     5581 cgaggacgtc gccatcgccg tccgcgagca cgcgcccaac gcggctgcgg tcgtgggcat
     5641 gtccctgggc ggtctgaccg cgctcgagct caccgtggtc gcgcccgagc tcgtgcgtcg
     5701 cctggtcatg gtcgacgtca cccccggggc cgaccgggag aaggcctccg a
//
LOCUS       DATISU010000124         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_844463, whole genome shotgun sequence.
ACCESSION   DATISU010000124 DATISU010000000
VERSION     DATISU010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844463"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..111
                     /gene="rpsK"
                     /locus_tag="VLR27_05195"
     CDS             <1..111
                     /gene="rpsK"
                     /locus_tag="VLR27_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017795137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HSP02873.1"
                     /translation="ETAIRSIQNAGIEVSGIKDVTPVPHNGCRPPKRRRT"
     gene            122..739
                     /gene="rpsD"
                     /locus_tag="VLR27_05200"
     CDS             122..739
                     /gene="rpsD"
                     /locus_tag="VLR27_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HSP02874.1"
                     /translation="MARYTGPRSRVARRLGVNIFGTAGEARALDRRPYPPGEHGRSRR
                     RGQPSEYLAQLQEKQKARFSYGLSERQFRNLYEEANRRKGVTGELMLQMLERRLDNVV
                     YRAGLAATRPQARQFVNHGHFDVNGKRVTIPSYRVRVGDKISLRTKSRDMVTVQWNKD
                     VLDRQAPGWLETGDDGFAVTVRAYPEREQIDVPVREQLIVELYSK"
     gene            796..1734
                     /locus_tag="VLR27_05205"
     CDS             796..1734
                     /locus_tag="VLR27_05205"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HSP02875.1"
                     /translation="MLVIQRPTVEPQGEEADNRQRFVISPLEPGFGHTIGNSLRRVLL
                     SSIPGAAVTQVRFDEALHEFDTIGGVVEDVTDIILNLKDLVVVCHSDDPVSLRLDARG
                     PAEVTAADLQDNADVEVLNPDLHIATMNGDGRLAIDVTIAKGRGYVSADRNKSSDTIG
                     VIPVDSIFSPVRKATFSVEPTRVEQDTNYDKLVIDVHTDGSITPAEALASAGDTLRSL
                     VGLIADMSEEPQGLELGEVPVTGTGPSDLDQPIEDLELSERPRNCLKRAQVNTIGELV
                     NKTEDDLLAITNFGQKSLDEVVEKLDERGLTLRIRG"
     gene            1744..>2048
                     /gene="rplQ"
                     /locus_tag="VLR27_05210"
     CDS             1744..>2048
                     /gene="rplQ"
                     /locus_tag="VLR27_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HSP02876.1"
                     /translation="MPARPRKGKRFGGSSSHQKAMFGNLVASLIAAESIVTTEAKAKA
                     VRPVAEKLITKAKKGGLHNHRQVVAYLRDEEMAHKLMTDVGPRYVDRPGGYTRILKLG
                     "
BASE COUNT          370 a          809 c          601 g          268 t
ORIGIN      
        1 gagaccgcca tccgctccat ccagaacgcc ggcatcgagg tgtccggcat caaggacgtc
       61 acccccgtcc cgcacaacgg ctgccgcccg cccaagcgcc gccgcacctg aggaacagaa
      121 catggctcga tacaccggac cccgcagccg cgtcgctcga cgactcggcg tcaacatctt
      181 cggcacggcc ggcgaggcgc gcgccctcga ccgccgcccc tacccgcccg gcgagcacgg
      241 tcgcagccgt cgccgcggcc agccctccga gtacctcgcc cagctgcagg agaagcagaa
      301 ggcccgcttc tcctacggcc tctccgagcg ccagttccgc aacctctacg aggaggcgaa
      361 ccgccgcaag ggcgtcaccg gcgagctcat gctccagatg ctcgagcgtc gcctcgacaa
      421 cgtcgtgtac cgggccggcc tcgccgctac ccggccccag gcacgccagt tcgtgaacca
      481 cggccacttc gacgtcaacg gcaagcgggt caccatcccc agctaccgcg tccgcgtcgg
      541 cgacaagatc tcgctgcgca ccaagtcccg cgacatggtc accgtgcagt ggaacaagga
      601 cgtcctcgac cgccaggccc cgggctggct cgagacgggc gacgacggct tcgccgtcac
      661 cgttcgggcc taccccgagc gtgagcagat cgacgtgccc gtgcgagagc agctcatcgt
      721 cgagctgtac tcgaagtagc actccgcacc gcccactccg cccctccttc tccacctgac
      781 gaggtacctc gctccatgct ggtcatccaa cgcccaaccg tcgagcccca gggtgaggag
      841 gccgacaacc gtcagcggtt cgtcatcagc cccctcgagc ccggcttcgg gcacaccatc
      901 ggcaactcgc tgcgccgcgt cctgctgtcc tcgatccccg gcgccgccgt cacccaggtg
      961 cgcttcgacg aggccctgca cgagttcgac acgatcggcg gtgtggtcga ggacgtcacc
     1021 gacatcatcc tcaacctcaa ggacctcgtc gtggtctgcc acagcgacga cccggtctcc
     1081 ctgcgcctcg acgcccgcgg ccccgccgag gtcaccgcgg ccgatctgca ggacaacgcc
     1141 gacgtggagg tcctcaaccc ggacctgcac atcgccacga tgaacggcga cggccgcctg
     1201 gccatcgacg tcaccatcgc caagggccgg ggctacgtgt ccgccgaccg caacaagagc
     1261 tccgacacca tcggcgtgat cccggtcgac tcgatcttct cgccggtgcg caaggccacc
     1321 ttctcggtcg agcccacccg cgtcgagcag gacaccaact acgacaagct cgtgatcgac
     1381 gtccacaccg acggctcgat caccccggcc gaggcgctcg cgtccgccgg cgacacgctg
     1441 cgcagcctgg tcggcctgat cgccgacatg agcgaggagc cccagggcct cgagctcggc
     1501 gaggtccccg tgaccggcac cggcccgtcc gacctcgacc agcccatcga ggacctcgag
     1561 ctgtccgagc gcccgcgcaa ctgcctcaag cgggcgcagg tcaacaccat cggtgagctc
     1621 gtcaacaaga ccgaagacga cctgctggcc atcacgaact tcggccagaa gtccctcgac
     1681 gaggtcgtcg agaagctgga tgagcgcggc ctcacgctgc gcatccgagg ctaggagaaa
     1741 gacatgcctg cacgtccccg caagggcaag cgcttcggcg gctcgtcctc ccaccagaag
     1801 gccatgttcg gcaacctggt ggccagcctc atcgccgccg agtcgatcgt gaccaccgag
     1861 gccaaggcca aggccgtccg cccggtggcc gagaagctca tcaccaaggc caagaagggc
     1921 ggtctccaca accaccgcca ggtcgtcgcc tacctgcgcg acgaggagat ggcccacaag
     1981 ctcatgaccg acgtcggccc ccgctacgtc gaccgtccgg gcggctacac ccggatcctc
     2041 aagctcgg
//
LOCUS       DATISU010000125         4143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_425215, whole genome shotgun sequence.
ACCESSION   DATISU010000125 DATISU010000000
VERSION     DATISU010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425215"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VLR27_05215"
     CDS             <1..914
                     /locus_tag="VLR27_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSP02877.1"
                     /translation="AASRSRVEGTKVTTWAADRDPYDPQRLAIAADLRDAIPAGELQV
                     HFQPQVDIESGLVTGAEALVRWRHPRLGELRPDQFIAAAEYTGAIDALTEHVLSVAAQ
                     TCAVWRQQGWNLDVSVNLSARNLANVAFADLVERILDDTGLDPAALTLELTESSIMTE
                     AEGAMKTLAAIHDLGVSLSIDDFGTGYSSLAHLRKLPVSEIKIDKSFVFDLTTSKSDE
                     AIVRSMVDLGHHLGLTVVVEGVETVEVRRRLADMGADRIQGYLLSRPMPVAAFEQWIA
                     DRPVRMAELSTSEAEADAPTRIEDRER"
     gene            complement(955..1653)
                     /locus_tag="VLR27_05220"
     CDS             complement(955..1653)
                     /locus_tag="VLR27_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HSP02878.1"
                     /translation="MQDPVRAVLEAGEIEIEGRMPWSSNGTFLVTVTHGDQWMRAIYK
                     PRRGERPLWDFPSGLDRREVAAHELSEALGWGLVPHTVLRDGPLGQGSVQRFVEADFE
                     QHYFTLFEAGRHHDRMQAMCAFDIVANNTDRKSGHCLVSVGDELFGIDHGLCFHEDFK
                     LRTVIWEFAGEPIPDDLIEDLARLRSCGLPDPLAALLEDHEQDAVLDRIDLLLRLGHF
                     PLDESGHRYPWPLV"
     gene            complement(1785..2255)
                     /locus_tag="VLR27_05225"
     CDS             complement(1785..2255)
                     /locus_tag="VLR27_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HSP02879.1"
                     /translation="MSEYLDPAAFTAGAIGPPGQRVFYLQACDNGLTSSVRLEKQQVS
                     ALAEYLAGMLHDLPAPSGVPAAPELEEPVEADWIVGNIGVAYDNDLDRIIIVAEELVA
                     EDEPDGDTLRVILSRSQVRAFIERASELLAGGRPPCRVCGAPMDPEGHACPRAN"
     gene            complement(2266..2979)
                     /locus_tag="VLR27_05230"
     CDS             complement(2266..2979)
                     /locus_tag="VLR27_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HSP02880.1"
                     /translation="MAATRTTKTSTKRTKKGDSAPKPTMVLLVRHGRTPTTGSVLPGR
                     ASGLHLADDGRAQAEAVAARIRSLHDKRTGSKDGVGVTAVYASPMERTQETAAPIAKA
                     LGLRTRTRRGLIECDFGEWTGRKLTDLYKLPEWKTVQRHPSGFRFPKGESFGEMQTRM
                     VDTVNELVAAHPGEIVVAVSHADTIKAAVASALGTPLDLFQRIVIGQCAVSAILYGAE
                     GPTVLAVNHTDDLVSLVPS"
     gene            3002..3682
                     /locus_tag="VLR27_05235"
     CDS             3002..3682
                     /locus_tag="VLR27_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HSP02881.1"
                     /translation="MDPFQTLAEDVASCRACPRLVAWREQVATEKRAAFRDEEYWGRP
                     VPGWGDPAATVVIVGLAPAAHGANRTGRMFTGDRSGDFLYAALHRTGFANQPTAIRPG
                     DGLELDGVWITSPVKCAPPANKPTPLERDTCATFLDRELTLLTEARVFVPLGQFGYQA
                     TCRFLGVRPRPKFGHGIEVEVPVAEHDAPRWIVPSYHVSQQNTFTGTLTEPMLDSVLE
                     RARELAAS"
     gene            complement(3679..>4143)
                     /locus_tag="VLR27_05240"
     CDS             complement(3679..>4143)
                     /locus_tag="VLR27_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HSP02882.1"
                     /translation="LLCSKTFDDAIFEELFETKYGIPKQRITKMNIKGVFQLWTDDGG
                     YHEIPLKECHAWTREGCTHCPDFAAEHADVSTGGIGKENDWTLTIVRTELGREVISRM
                     ITAGVIEARPGDEDPGAIALMHKLAEKSRKRWPEFAVDFPKAKREVTFASEG"
BASE COUNT          583 a         1459 c         1469 g          632 t
ORIGIN      
        1 tggccgccag ccgctcccgg gtcgagggca ccaaggtcac gacctgggcc gctgaccgcg
       61 acccctacga cccgcagcgc ctggccatcg ccgccgacct gcgcgacgcc atccccgccg
      121 gcgagctcca ggtgcacttc cagccccagg tcgacatcga gagcgggctg gtgacgggtg
      181 cggaggcgct ggtccgctgg cgccaccccc ggctgggcga gctgcgcccc gaccagttca
      241 tcgcggccgc cgagtacacc ggcgccatcg acgccctcac cgagcacgtg ctgtccgtcg
      301 ccgcccagac gtgtgcggtc tggcggcagc agggctggaa cctcgacgtc tccgtcaacc
      361 tctcggctcg caacctcgcc aacgtcgcct tcgccgacct ggtcgagcgc atcctggacg
      421 acaccggcct ggacccggcc gccctcaccc tcgagctcac cgagtcgagc atcatgaccg
      481 aggccgaggg ggcgatgaag acgctcgccg ccatccacga cctgggcgtc tcgctctcga
      541 tcgacgactt cggcaccggc tactcctcgc tggcgcacct gcggaagctg ccggtgtcgg
      601 agatcaagat cgacaagtcc ttcgtcttcg acctcacgac cagcaagagc gacgaggcca
      661 tcgtccgctc gatggtcgac ctcggccacc acctcggcct caccgtcgtc gtcgagggcg
      721 tggagaccgt cgaggtccgc cgtcgcctcg ccgacatggg cgccgaccgc atccagggct
      781 acctgctcag ccgccccatg cccgtggcgg ccttcgagca gtggatcgcc gaccgccccg
      841 tccgcatggc cgagctgtcc accagcgagg ccgaggccga cgcgccgacc cggatcgagg
      901 accgggagcg ctgaccttcg cgctacacca gcactgccag ttcgagaagg ctggtcagac
      961 gaggggccag gggtagcggt ggccgctctc gtccaggggg aagtggccga gccgcaggag
     1021 caggtcgatc cggtcgagga ccgcgtcctg ctcgtggtcc tcgagcaggg cggccaacgg
     1081 gtcggggagc ccgcagctgc gaaggcgggc caggtcctcg atgaggtcgt cggggatggg
     1141 ttcgccggcg aactcccaga tcaccgtgcg cagcttgaag tcttcgtgga agcagaggcc
     1201 gtggtcgatg ccgaacagct cgtcgcccac cgagacgagg cagtggccgc tcttgcggtc
     1261 ggtgttgttg gccacgatgt cgaacgcgca catggcctgc atccgatcgt ggtgccggcc
     1321 ggcctcgaag agggtgaagt agtgctgctc gaagtcggcc tcgacgaagc gctgcacgga
     1381 cccctggccg agcggaccgt cccgcaacac cgtgtggggc accaggcccc agccgagcgc
     1441 ctcgctcagc tcgtgggcgg cgacctcacg acggtcgagc ccactgggga agtcccacag
     1501 cggccgctcg ccccgtcggg gcttgtagat cgcccgcatc cactggtccc cgtgggtgac
     1561 cgtgaccagg aaggtgccgt tcgacgacca gggcatccgg ccctcgatct cgatctcacc
     1621 cgcctcgagg acggcacgca cggggtcctg catcaccgat gatcctggcg cgtgggcgac
     1681 cgcgaacgcg agcgggcgtc aggatctgcg cggacaggtc acgtcccgcc gcggctcggt
     1741 tcggcggctc cgccgccatg gcgaccgtga catgggtcgc ctcctcaatt ggcccggggg
     1801 caggcgtgcc cctcggggtc catcggcgcg ccgcagacgc ggcacggagg acggccgccg
     1861 gccagcagct cgctggcccg ctcgatgaag gcccgcacct gcgagcggct gaggatgacc
     1921 cgcagggtgt ccccgtcggg ctcgtcctcg gccactagct cctcggcaac gatgatgatc
     1981 cggtcgaggt cgttgtcgta ggccacgccg atgttgccca cgatccagtc ggcctccact
     2041 ggctcctcca gctcgggggc ggcagggacc ccggacgggg cgggcaggtc gtggagcatg
     2101 ccggcgaggt actccgccag cgcgctcacc tgctgcttct ccagccgcac ggagctggtc
     2161 aagccgttgt cgcacgcctg caggtagaag acccgctggc cgggtggtcc gatggcgcca
     2221 gcggtgaagg cagcagggtc gaggtactcg ctcatgccgt cctcctcatg atgggacgag
     2281 ggagacgagg tcgtcggtgt ggttcacggc gaggaccgtg gggccctcgg cgccgtacag
     2341 gatcgccgac acggcgcact ggccgatgac gatgcgctgg aacaggtcga gcggcgtgcc
     2401 gagggcggac gccaccgccg ccttgatcgt gtcggcgtgg gacacggcga cgacgatctc
     2461 gcccgggtgg gcggcgacca gctcgttgac ggtgtcgacc atgcgagtct gcatctcgcc
     2521 gaacgactcg cccttgggga accggaagcc cgacgggtgg cgctgcacgg tcttccactc
     2581 gggcagcttg tagagatcgg tcagcttgcg gccggtccac tcgccgaagt cgcactcgat
     2641 gaggcccctg cgggtccggg tgcgcaggcc gagcgccttg gcgatcgggg cggccgtctc
     2701 ctgggtgcgc tccatcggcg aggcgtacac cgcggtgacg ccgaccccgt ccttggagcc
     2761 ggtccgtttg tcgtgcagcg aacggatccg tgcggcgacg gcctcggcct gggcccggcc
     2821 gtcgtcggcg aggtggagac cgctggctcg cccgggcagg acactgccgg tggtgggggt
     2881 gcgcccgtgg cgcaccagca gcaccatggt cggcttgggt gcgctgtcgc ccttcttcgt
     2941 cctcttggtc gaggtcttgg tcgtgcgcgt ggcagccatg caatgggcca cgctatcggc
     3001 catggatccg ttccagaccc tggccgagga cgtcgcgagc tgccgcgcct gcccccgtct
     3061 ggtggcctgg cgggagcagg tcgcgaccga gaagcgggcg gcgttccggg acgaggagta
     3121 ctggggtcga cccgtcccgg gctggggcga tccggcggcc acggtcgtca tcgtgggcct
     3181 cgcgccggcc gcccacggcg ccaaccgcac cggtcggatg ttcaccggcg accgctccgg
     3241 tgacttcctc tacgccgccc tgcaccgcac cgggttcgcc aaccagccga cggcgatccg
     3301 acccggtgac gggctcgagc tggacggcgt ctggatcacg tcaccggtga agtgcgcgcc
     3361 gccggcgaac aagccgaccc cgctggagcg ggacacgtgc gccacgttcc tcgaccggga
     3421 gctgacgctg ctcaccgagg cgcgggtgtt cgtcccgctc ggccagttcg gctaccaggc
     3481 gacgtgccgg ttcctcggcg tgcggccccg cccgaagttc gggcacggca tcgaggtgga
     3541 ggtgccggtg gcggagcacg acgctcctcg ctggatcgtg cccagctacc acgtcagcca
     3601 gcagaacacc ttcaccggga ccctcaccga gccgatgctc gactcggtgc tggaacgagc
     3661 ccgggagctc gccgcttcct agccctcgct ggcgaaggtg acctcgcgct tggccttggg
     3721 gaagtcgacg gcgaactcgg gccagcgctt gcgcgacttc tcggccagct tgtgcatcag
     3781 cgcgatggcg cccgggtcct cgtcgccggg gcgggcctcg atgacccccg cggtgatcat
     3841 gcgggagatg acctctcgcc cgagctcggt gcgcacgatg gtgagggtcc agtcgttctc
     3901 cttgccgatg ccgccggtgg agacgtcggc gtgctcggcg gcgaagtccg ggcagtgggt
     3961 gcagccctcg cgggtccagg cgtggcactc cttcagcggg atctcgtggt agccgccgtc
     4021 gtcggtccag agctggaaga cgcccttgat gttcatcttc gtgatgcgct gcttcgggat
     4081 gccgtacttg gtctcgaaca gctcctcgaa gatggcgtcg tcgaacgtct tggagcacag
     4141 cag
//
LOCUS       DATISU010000126         7564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_378768, whole genome shotgun sequence.
ACCESSION   DATISU010000126 DATISU010000000
VERSION     DATISU010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378768"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VLR27_05245"
     CDS             <1..234
                     /locus_tag="VLR27_05245"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HSP02883.1"
                     /translation="EIDLVLRRGDTLVFAEVKTRTSDRFGHPAEAVTVPMQRRIRGLA
                     QRFCAETGNRARVLRFDVVSVLRGQVERYEGCF"
     gene            369..1145
                     /locus_tag="VLR27_05250"
     CDS             369..1145
                     /locus_tag="VLR27_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HSP02884.1"
                     /translation="METLQVERDGGIVTVTLNKPEKKNAVDPTQWTEMARIWDDIARN
                     PRRDRAVIVTGAGGDFCSGADLWLPPGEEPPHQMTAMRSVAKAIQGLHDLPQPTIAKV
                     PGVAAGAGFSLALGCDLVVAGEAARFSMIFAKRGLALDGGASWLLPRLLPLHKAKELA
                     FFADMLSAEQAEDFGLVNHVVPDGELDGFVADWAARLAGGPPIALGLTKRLLNDSYSR
                     TLQEALEAEGMAQSITLASKDTAEAIASFMEKRDPTFHGR"
     gene            1259..2770
                     /locus_tag="VLR27_05255"
     CDS             1259..2770
                     /locus_tag="VLR27_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HSP02885.1"
                     /translation="MLASVPAATLLGVTGHPVDVEVYVGNGLPSLSIVGLPDASCREA
                     RDRVRAAVLSSDLEWPPHRITVNLAPSALHKLGTGLDLALAVGVLVATGEVSAEAVAR
                     WAFLGELGLDGSVRTVRGMVPLVAGVRDRVDGVVVAPAAVAEASVIPGLEVRCAATLT
                     ELVAALRVERPWPDPPEAPVVEEPPPPDIADVRGQVLGRRAMEIAAAGGHHVLLAGPP
                     GSGKTLLATRLPGLLPDLDDDVALEVTQIHSAAGATPSTGLVRRPPFRAPHHTASAVA
                     LIGGGTVAMRPGEVSCAHGGVLFCDELGEFPPSVLDALRQPLEDGVVRVSRARASVTY
                     PARFQLVAATNPCPCGPAPGGCRCSEVSRARYLRRLSGPLLDRFDLRVEVDRPEPGDL
                     LGGEPGEPSAVVAGRVRAARECAAERGVGCNALLPAPQLERWAPLTASARRLLEALVR
                     DGRLSGRGVQRIRRVARTAADLDGRDGPLDDEQVAIALALRSSAGTAALGVAS"
     gene            2767..3912
                     /gene="dprA"
                     /locus_tag="VLR27_05260"
     CDS             2767..3912
                     /gene="dprA"
                     /locus_tag="VLR27_05260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HSP02886.1"
                     /translation="MTAVAPRDDERAVLATLLGLPGMGPARLTAVVDRAGSAVEAWEL
                     VRRGDLDGIDLRSVQKRAELVDGWVQTARRLDPAVVLAQHRAAGVEVLLTGDATWPSV
                     FTLDPEPPRVLFVRGDATLLEQPGVAVVGTRRCTAGGVQIAGRFGAGLADAGLTVVSG
                     LALGVDGAAHRGALGAGGRPVGVVGSGLDVVYPARHRRLWADVAERGVLVGEAPLGSP
                     PERWRFPARNRIIAALSLAVVVVESGARGGSMTTVEEAATRDVPVLAVPGSVLAEQSV
                     GTNQLIFDGSGIARDVADVLAALGVQVPTGPPRLFDDEAASGAPGTGGGAVGAVAAVL
                     SAVPASVEQVATAADVSVTEAVTALARLEASGAVRRVPGGYERVAVP"
     gene            3917..4810
                     /locus_tag="VLR27_05265"
     CDS             3917..4810
                     /locus_tag="VLR27_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HSP02887.1"
                     /translation="MAWQVEQFGASLTDAAASTARAYETDVAAFVEFADRMGLDDPAD
                     VTRLHVRRWVAQLATRDLARATIARRVAALRRYFGWLQRRGVVSADPTVGLRAPAGGS
                     RLPRVLRQDELDVLLDQPPAAVDDDPPHRRVRDDAVIELLYGSGLRVAEVCGLRADDV
                     DLTAGTVRVLGKRSKWRQVPLTPGAVEAIDAWLRTGRAELATAQSPDDLVFLNLRGRA
                     LTPRDCRRILDRRAVDPTHPHALRHTFATHLLDGGADLRAVQELLGHSDLSTTQRYTH
                     VSASRLQAVVAASHPRAKGCS"
     gene            4807..5586
                     /gene="whiG"
                     /locus_tag="VLR27_05270"
     CDS             4807..5586
                     /gene="whiG"
                     /locus_tag="VLR27_05270"
                     /inference="COORDINATES: protein motif:HMM:NF004935.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HSP02888.1"
                     /translation="MTELASADIDRLWSQYKDSGSRRARDQLIVHYSPLVKYVAGRVA
                     VGLPQNVDQADLVSYGLFGLIDAIDKFEPERGFKFETYAISRIKGNILDELRAIDWVP
                     RSVRAKARQIEKAYAKLEAQHHRAPSEDELAQELDMSREQLQTSLSKISFVGVVALDE
                     FVGSGDREGSATLGDTIATADEGPGDLFEVQEMRGLLGDSINRLPEREKVVLTLYYYE
                     GLTLAQIGEVLGVTESRVCQIHTKAILQLRSRMQAADRETV"
     gene            5687..7339
                     /locus_tag="VLR27_05275"
     CDS             5687..7339
                     /locus_tag="VLR27_05275"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HSP02889.1"
                     /translation="MSMRAARPTRRVAAVAVALATAFIVVVPADPAGAAPPDPVVHAP
                     PVDAPVADPFRPPATTYGPGNRGLAYDLAPGTPVRATADGTVAFAGPVGSTLHVTVLH
                     ADGLRTSYSFLSEVDVQRGDRVDRGAVVGRAGPGFHLGARDGEHYLDPASLFGEPVIE
                     VRLVPHGQPLPPTDAGLLAEQSWLRDLVRAEKPGFLRRMASAVVRHGAPIVERFAGTV
                     DAAWHTWTQLNPVQVTAGVVTSLARHLRQECTAASVVPAPPAGERVALLVAGLGSSSD
                     HGAIDDVDLDALGYAGSDVLRYRYGGGRTPGSGDLHPDLAGIPAAAYVPEDTLGDVTE
                     RGRELADLIEHAAAARPGVPIDVYAHSLGGLVTRVALLELAERRDRGQSTAIDALGPV
                     ATIGSPHRGADLATVAVLSEKGFVQDVAHIRSIFDIPIDPYSPAVRQLAETSALVQRL
                     GIQGVPDGVELRTIAARGDLVVAADKADVDGRPAAIVDLSGPHAHAALPGDPDTTREL
                     QLGLAGLPPACQGFGDAVLDAVVPEIVAGVTDVAGLLTLLPS"
BASE COUNT          909 a         2975 c         2676 g         1004 t
ORIGIN      
        1 gagatcgacc tggtcctgcg gcggggcgac accctggtgt tcgccgaggt caagacccgc
       61 acctcggacc gcttcggcca cccggccgag gccgtcaccg tgcccatgca gcgccgcatc
      121 cggggcctcg cccagcgctt ctgcgccgag accgggaacc gggcccgggt gctgcgcttc
      181 gacgtggtca gcgtgctgcg cggccaggtc gagcgctacg agggctgctt ctagatcagc
      241 cgaagaacgc ccacagcacg agcagcacgg cgacgatgag gccggcggcc acgaacacca
      301 ggtcgctgcg gcggtgcttc tgggggcggt gggggttgaa gcccatggtc gaacactagg
      361 ttcccgccat ggaaaccctg caggtcgagc gcgacggcgg catcgtcacc gtcacgctga
      421 acaagcccga gaagaagaac gccgtcgacc ccacgcagtg gaccgagatg gcccgcatct
      481 gggacgacat cgcccgcaac ccccgccgag atcgggccgt gatcgtcacc ggcgcgggtg
      541 gcgacttctg ctcgggtgcc gacctgtggc tcccgccggg cgaggagccg ccccaccaga
      601 tgaccgccat gcgcagcgtg gccaaggcga tccagggtct ccacgacctg ccccagccga
      661 ccatcgccaa ggtgcccggc gtcgccgccg gcgccggctt ctccctcgcg ctcggatgtg
      721 acctggtggt cgccggtgag gccgctcgct tctcgatgat cttcgccaag cgcggcctgg
      781 ccctggacgg cggcgcatcg tggctgctgc cccggctgct gccgttgcac aaggccaagg
      841 agctcgcgtt cttcgccgac atgctgtcgg cggagcaggc cgaggacttc ggcctggtca
      901 accacgtcgt gcccgacggc gagctcgacg gcttcgtcgc cgactgggcg gcgcgtctcg
      961 ccggcggtcc gcccatcgcg ctgggcctga cgaagcggct cctgaacgac tcgtactcgc
     1021 gcaccctgca ggaggccctc gaagccgagg gcatggccca gtcgatcacg ctggcgtcga
     1081 aggacacggc ggaggcgatc gccagcttca tggagaagcg ggacccgacc ttccacgggc
     1141 gctaggccgg gttcctgtcg gtgacccgcg tgtagggttc cgccatcgac cggttcgtcc
     1201 ggtccgctcg atgcgccggc gcacggccgt ccactccgca ccgctggagg gaccaaccgt
     1261 gctcgcatcc gtcccggccg ccacgctcct cggcgtcacc ggccaccccg tcgatgtcga
     1321 ggtctacgtc ggcaacggcc tgccgtcgct gtccatcgtc ggcctgccgg acgcgtcctg
     1381 ccgcgaggca cgtgaccgcg tccgggccgc cgtgctgtcg agcgacctcg agtggccgcc
     1441 gcaccgcatc acggtgaacc tcgcgccgtc ggcgctccac aagctcggca ccgggctcga
     1501 cctcgctctc gccgtcggcg tgctcgtcgc caccggcgag gtgtcggccg aggccgtggc
     1561 gcgctgggcg ttcctcggcg agctcggact cgacggctcg gtgcgcaccg tccgcgggat
     1621 ggtcccgctc gtcgccggcg tccgcgaccg ggtcgacggc gtggtcgtgg cgcctgccgc
     1681 ggtggccgag gccagcgtga tccccggtct cgaggtgcgg tgcgccgcca ccctcaccga
     1741 gctggtcgcc gcgctccggg tcgagcgtcc ctggcccgac ccgcccgagg caccggtcgt
     1801 ggaggaaccg ccgcccccgg acatcgccga cgtccgggga caggtcctgg gtcgtcgcgc
     1861 catggagatc gccgccgccg gcgggcacca cgtgctgctc gccgggccgc cgggttcggg
     1921 caagacgctg cttgccaccc ggctgcccgg cctgctgccc gacctcgacg acgacgtcgc
     1981 cctcgaggtc acccagatcc actcggcggc cggtgccacg ccgtccacag ggctggtgcg
     2041 gcgcccgccc ttccgcgccc cgcatcacac ggcgtccgcc gtcgccctca tcggtggggg
     2101 cacggtggcg atgcggcccg gcgaggtctc ctgcgcccac ggcggggtcc tcttctgcga
     2161 cgagctgggt gagttcccgc cgtcggtgct cgacgccctc cgccaacccc tggaggacgg
     2221 cgtggtccgg gtcagccggg cccgggcgtc ggtcacctac ccggcgcggt tccagctggt
     2281 cgccgccacc aacccgtgcc cgtgcgggcc ggcgcccggt gggtgccggt gctccgaggt
     2341 ctcccgcgcc cggtacctgc gccggctctc gggaccgctg ctcgaccgct tcgacctgcg
     2401 ggtggaggtc gaccggcccg aacccggcga cctcctcggc ggcgaaccgg gcgagccgtc
     2461 ggccgtcgtg gccgggcggg tgcgcgccgc ccgggagtgc gccgccgagc gcggcgtcgg
     2521 ctgcaacgcc ctgctccccg ccccgcagct ggagcggtgg gcgccgctga cggcctcggc
     2581 ccgccggctc ctggaggcgc tcgtgcgcga cggccgcctc tccggccgtg gcgtgcagcg
     2641 catccgccgc gtcgcccgca cggcagcaga cctcgacggg cgcgacggcc cgctcgacga
     2701 cgagcaggtg gccatcgccc tcgccctgcg ctccagcgcc ggcaccgcag ccctgggggt
     2761 ggcgtcgtga ccgcggtcgc gcccagagat gacgagcgcg ccgtgctcgc cacgctgctc
     2821 ggcctgccgg ggatgggtcc ggcgcgcctc acggcggtcg tggaccgagc tgggagcgcc
     2881 gtggaggcct gggagctcgt gcgccggggc gacctcgacg gcatcgacct gcgcagcgtc
     2941 cagaagcggg ccgagctcgt cgacgggtgg gtgcagacgg cccgccgcct cgatcccgcc
     3001 gtcgtgctcg cccagcaccg ggccgccggc gtcgaggtgc tgctcacggg ggacgcgacc
     3061 tggccgtcgg tgttcaccct cgacccggag ccaccgcggg tcctgttcgt ccgtggcgac
     3121 gccaccctgc tcgagcagcc cggcgtggcg gtcgtgggca cccgtcggtg caccgccggc
     3181 ggggtccaga tcgccggtcg gttcggtgcc ggtctcgccg acgccgggct cacggtggtg
     3241 tcgggactcg ccctcggagt cgacggcgca gcccaccgcg gtgcgctcgg cgccggcggc
     3301 cggccggtcg gcgtcgtcgg cagcgggctc gacgtcgtgt accccgcccg gcaccgccgc
     3361 ctgtgggccg acgtagccga gcgcggggtc ctcgtgggag aggcgccgct cggctccccg
     3421 ccggagcgat ggcgcttccc ggcgcggaac cggatcatcg ccgcgctgtc cctcgccgtg
     3481 gtggtcgtgg agtccggtgc ccggggcggc tcgatgacca ccgtcgagga ggccgccacc
     3541 cgtgacgtcc ccgtcctcgc cgtccccggg tcggtgctgg ccgagcagtc ggtcggcacg
     3601 aaccagctga tcttcgacgg ctccggcatc gcccgcgacg tcgccgatgt gctggcggcc
     3661 ctcggtgtgc aggtgccgac cgggccgccg cggctcttcg acgacgaggc agcgtcgggc
     3721 gcacccggga cgggcggggg agcggtgggc gcggtcgcgg ccgtgctctc cgccgtgccg
     3781 gcgagcgtcg agcaggtggc cacggctgcg gacgtgagcg tcacggaggc ggtgaccgcc
     3841 ctcgcccggc tggaggcatc gggtgcggtg cggcgggtcc ctgggggcta cgagcgggtg
     3901 gcggtaccgt gatcggatgg cgtggcaggt ggagcagttc ggcgcatccc tcaccgatgc
     3961 cgccgcgtcc acagcgcggg cctacgagac cgacgtcgcc gcgttcgtgg agttcgccga
     4021 tcgcatgggc ctcgacgacc cggccgacgt cacccggctc cacgtccgcc ggtgggtcgc
     4081 ccagctcgcc acccgcgacc tggcccgggc caccatcgcc cgtcgggtgg ccgccctgcg
     4141 ccgctacttc ggctggctgc agcgccgtgg tgtcgtctcc gccgacccca ccgtcggcct
     4201 ccgagcgccg gcgggcggca gcaggttgcc tcgcgtcctg cgccaggacg agctcgacgt
     4261 cctgctcgac cagcccccgg cagccgtcga cgacgacccg ccccaccgcc gcgtgcgcga
     4321 cgacgccgtc atcgagctgc tctacggcag cggcctgcgg gtggccgagg tctgcggcct
     4381 gcgggccgac gacgtcgacc tcaccgccgg caccgtccgg gtgctgggca agcgctcgaa
     4441 gtggcgccag gtgcccctca cgccgggtgc ggtggaggcc atcgacgcct ggctgcgcac
     4501 cgggcgcgcc gagctggcga cggcgcagtc accggacgat ctggtcttcc tcaacctccg
     4561 gggccgggcg ctcacacccc gcgactgccg ccgcatcctc gatcggcgcg ccgtcgatcc
     4621 cacccacccc cacgccctgc gccacacctt cgccacgcac ctgctcgacg gcggcgcgga
     4681 cctgcgcgcc gtccaggagc tcctgggcca cagcgacctg tccaccacgc agcggtacac
     4741 tcacgtcagc gccagccgtc tgcaggcggt ggtggccgcc tcccacccac gtgccaaggg
     4801 ctgctcgtga ccgaactcgc ttccgcagac atcgaccgcc tctggtcgca gtacaaggac
     4861 agcggttccc gtcgtgcccg cgaccagctc atcgtgcact actcgccgct ggtcaagtac
     4921 gtggccggtc gcgtcgccgt cggtctgccc cagaacgtcg accaggccga ccttgtctcc
     4981 tacgggctgt tcggcctcat cgacgccatc gacaagttcg agcccgagcg cggcttcaag
     5041 ttcgagacct acgccatctc ccgcatcaag ggcaacatcc tcgacgagct gcgcgccatc
     5101 gactgggtgc cccgctccgt gcgggccaag gcccgccaga tcgagaaggc ctacgccaag
     5161 ctcgaggccc agcaccaccg ggcccccagc gaggacgagc tcgcccagga gctcgacatg
     5221 agccgcgagc agctccagac gtcgctgtcg aagatctcct tcgtcggcgt cgtcgccctc
     5281 gacgagttcg tcggcagcgg cgaccgcgag ggcagcgcca ccctcggcga caccatcgcc
     5341 accgccgacg agggcccggg cgacctgttc gaggtccagg agatgcgcgg gctgctcggc
     5401 gactccatca accggctccc cgagcgggag aaggtcgtgc tgaccctcta ctactacgag
     5461 ggcctgacgc tggcgcagat cggcgaggtg ctcggcgtca ccgagtcccg ggtctgccag
     5521 atccacacca aggccatcct gcagctccgc tcgcgcatgc aggccgccga ccgcgaaacg
     5581 gtctgacgga cgggcgcccg gcagcgcccc ctgagcacca cggcttcccc cagccccggc
     5641 cgcaccgccc tcccggcgac ggccgtcgag ccgaggggga gcccccatgt ccatgcgcgc
     5701 tgcgcgcccg acccgccggg tcgcggcggt cgcggtcgcc ctggccaccg cgttcatcgt
     5761 cgtcgtccct gccgatcctg cgggcgcggc accgccggac ccggtcgtcc acgccccgcc
     5821 cgtggatgcc ccggtcgccg atccgttccg cccaccggcc acgacctacg gcccgggcaa
     5881 ccggggcctg gcctacgacc tggcgccggg caccccggtc cgggcgacgg ccgacggcac
     5941 cgtcgccttc gccgggccgg tcggctccac cctgcacgtc accgtcctgc acgccgacgg
     6001 gctgcgcacc tcctactcct tcctgtccga ggtcgacgtc cagcgaggcg accgggtcga
     6061 ccggggcgcg gtggtgggcc gggccggccc gggattccac ctcggggccc gcgacggcga
     6121 gcactacctc gatccggcgt cgctgttcgg tgagccggtc atcgaggtcc gcctcgtccc
     6181 ccacggtcag ccgctgccgc ccaccgatgc cgggctgctc gccgagcagt cgtggctgcg
     6241 cgacctggtc cgggccgaga agcccgggtt cctgcgacgg atggccagtg ccgtcgtccg
     6301 ccacggcgcg cccatcgtcg agcgcttcgc cggaacggtc gacgcggcat ggcacacgtg
     6361 gacccagctc aacccggtcc aggtcaccgc cggcgtcgtc acgtccctgg cccggcacct
     6421 ccgccaggag tgcaccgccg cctcggtcgt cccggcgccg ccggcggggg agcgggtggc
     6481 gctgctcgtc gccgggctgg gctcgtccag cgaccacggc gccatcgacg acgtcgacct
     6541 cgacgccctc ggctacgccg ggagcgacgt cctgcgctac cgctacggcg gcggccgcac
     6601 gccgggatcc ggtgacctcc accccgacct ggccggcatc cccgccgccg cctacgtgcc
     6661 cgaggacacc ctcggagacg tcaccgagcg ggggagggag ctggccgacc tgatcgagca
     6721 cgccgcggcg gcccgcccgg gcgtgcccat cgacgtctac gcccacagcc tcggcggcct
     6781 cgtcacccgg gtcgccctgc tcgagctcgc cgagcgacgg gatcgagggc agagcaccgc
     6841 catcgacgcg ctcggcccgg tcgccaccat cgggtcgccg caccggggcg ccgacctcgc
     6901 caccgtcgcc gtcctgtcgg agaaggggtt cgtgcaggac gtggcccaca tccgctccat
     6961 cttcgacatc ccgatcgacc cctacagccc ggcggtccgc cagctcgccg agacctcggc
     7021 gctggtgcag cgcctcggca tccagggcgt ccccgacggg gtcgagctgc gcacgatcgc
     7081 cgctcggggt gacctcgtgg tggccgccga caaggccgac gtggacggcc ggcccgccgc
     7141 catcgtcgac ctgagcggac cccacgccca cgcggcgctg cccggcgacc cggatacgac
     7201 gcgggagctg cagctgggcc tggccgggct cccgcctgcg tgccaggggt tcggcgacgc
     7261 cgtgctcgac gccgtggtcc ccgagatcgt cgctggcgtc accgacgtcg ccgggctcct
     7321 cacgctgctg ccgagctgag cccgccgggt gggtttccca gggccgctgc ggctccctac
     7381 actggcaagt cggcctgcgc acccgcgtcg gccacgtcca aatccaccga tcgggtcacc
     7441 ccacggtcgc aaccccctcc acgaggggcg cagcgccggc ccggtcgcag tgcaaccgag
     7501 gggcaacgcg aggagaagcc ggtggccgtc gtcaccatga agcagctgct ggaggccgga
     7561 gtcc
//
LOCUS       DATISU010000127         1637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_564984, whole genome shotgun sequence.
ACCESSION   DATISU010000127 DATISU010000000
VERSION     DATISU010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564984"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VLR27_05280"
     CDS             complement(<1..1035)
                     /locus_tag="VLR27_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HSP02890.1"
                     /translation="MSGVQVKSERAPVAFDMADATFRRDPYPRYAELRESDVLHRTPD
                     GFWVLTRHADVLAAVRDQRLSSNPVHIDRDPTSAREAGLPFLGDMGIQLLLTADPPDH
                     TRLRRLANKAFTPRAVERLRPRVTEIVDQLLDAATTDGATFDVMVDLAEPLPVIVICE
                     LLGVPVEDQAQFKPWSSTVSRIIDPDVTPDMVADAIPSIMGFVQYFGGLIEERRADPR
                     DDLLSSLIAAEAEGDTLGQQELMAMIILLFIAGHETTTNLIGNGTLALLRNPDQLAAL
                     RDDSGLAASATEELLRYDPAAQLTVRTATEDLDLHGIPLTKGESVVCGLAAGNRDPRA
                     FDDPDIVDITR"
     gene            1093..1554
                     /gene="bcp"
                     /locus_tag="VLR27_05285"
     CDS             1093..1554
                     /gene="bcp"
                     /locus_tag="VLR27_05285"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HSP02891.1"
                     /translation="MAPQAGDKAPAFTLPDQDGEKVKLTDLKGRKVLVYFYPKADTPG
                     CTTQSCELSAIKDDIDVQIIGISPDAPEKQKKFDEKHSLGFPLLSDPEHKVAEKYGSW
                     GEKSMYGKKYMGIIRSAFLIDEKGKVLEAWPKISPKDTPKKLLKALEIRRP"
BASE COUNT          266 a          552 c          579 g          240 t
ORIGIN      
        1 ccgggtgatg tcgacgatgt cggggtcgtc gaacgcccgg gggtcgcggt tgccggcggc
       61 gaggccgcac accacgctct cgcccttggt cagcgggatg ccgtgcaggt cgaggtcctc
      121 ggttgcggtg cgcaccgtga gctgggcggc cgggtcgtac cggagcagct cctccgtggc
      181 ggaggcggcc agcccggaat cgtcccgcag ggcggccagc tggtccgggt tccggagcag
      241 ggccagggtc ccgttgccga tcaggttggt ggtggtctcg tgcccggcga tgaacagcag
      301 gatgatcatc gccatcagct cctgctggcc cagcgtgtcg ccctcggcct cggcggcgat
      361 gagcgagctg agcaggtcgt cccgggggtc ggcccgccgc tcctcgatga gcccgccgaa
      421 gtactggacg aagcccatga tcgacgggat ggcatcggcg accatgtccg gggtgacgtc
      481 cgggtcgatg atgcgcgaca cggtggacga ccacggcttg aactgggcct ggtcctcgac
      541 cggcacgccg agcagctcgc agatcacgat caccggcagc ggctcggcca ggtcgaccat
      601 cacgtcgaag gtcgccccgt cggtcgtggc ggcgtcgagc agctgatcga cgatctcggt
      661 gacccgcggc cgcaggcgct cgaccgcacg aggggtgaac gccttgttgg cgaggcggcg
      721 caggcgggtg tggtcgggcg ggtcggcggt gaggagcagc tggatgccca tgtcgccgag
      781 gaaggggagg ccggcctccc gggcgctggt cgggtcccga tcgatgtgca ccgggttgga
      841 cgacaggcgc tggtcgcgca cggcggccag tacgtcggcg tggcgggtca gcacccagaa
      901 cccgtcgggc gtccggtgga ggacgtcgga ctcccgcagc tcggcgtagc gggggtacgg
      961 gtcccgccgg aacgtcgcgt cggccatgtc gaacgccacc ggcgcccgct cggacttcac
     1021 ctgcacccca gacatgtccc gatcgtactg ttaccgacgg ttacatgtcg atgggcgtgc
     1081 catcatcgcc gcatggctcc tcaagctggc gacaaggccc ccgcgttcac cctccccgac
     1141 caggacggcg agaaggtgaa gctcaccgac ctcaagggcc gcaaggtgct cgtgtacttc
     1201 tacccgaagg ccgacacgcc cggctgcacc acgcagtcct gcgagctctc ggccatcaag
     1261 gacgacatcg acgtccagat catcggcatc agccccgacg cgccggagaa gcagaagaag
     1321 ttcgacgaga agcactccct cggcttcccg ctgctctccg accccgagca caaggtggcc
     1381 gagaagtacg ggtcgtgggg cgagaagtcc atgtacggca agaagtacat gggcatcatc
     1441 cgctcggcgt tcctcatcga cgagaagggc aaggtcctcg aggcgtggcc caagatcagc
     1501 cccaaggaca ccccgaagaa gctgctcaag gccctcgaga tccgtcggcc gtgaccgacc
     1561 tcgacccccg ccaggtcctc ccgcaccgcg acccgttcgc gttcatcacc accatcgacg
     1621 aggtcgtgcc gggcgag
//
LOCUS       DATISU010000128         5875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_518466, whole genome shotgun sequence.
ACCESSION   DATISU010000128 DATISU010000000
VERSION     DATISU010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518466"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..910)
                     /gene="tatC"
                     /locus_tag="VLR27_05290"
     CDS             complement(194..910)
                     /gene="tatC"
                     /locus_tag="VLR27_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HSP02892.1"
                     /translation="MLDHLSELRDRIIKSAAAVAVGAVVCWFLYPAIFDVLVDPYCNL
                     QGATLDDCRLLQTEPLEAFSIRLKIAGYGGIALAMPVLLWQVWRFVVPGLHANEKRWA
                     YPFVGSALVLFVLGAALAFHTLTPALEFLTEVGGEGLQQQFRPAPYFELITYMMLAFG
                     VGFEFPIVLVFLQLAGVVTASSLRSARRYALVGIVVVVAVITPSGDPYTLGILSIPMY
                     LFYEASILIGMLLTRRSHDR"
     gene            complement(989..1198)
                     /gene="tatB"
                     /locus_tag="VLR27_05295"
     CDS             complement(989..1198)
                     /gene="tatB"
                     /locus_tag="VLR27_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HSP02893.1"
                     /translation="MFNIGGLELVAIALVALVVLGPDKLPAALRQAGALLGQVRRMSD
                     SFRIDVNAALAEDAVDRPGAEPRID"
     gene            complement(1224..1400)
                     /locus_tag="VLR27_05300"
     CDS             complement(1224..1400)
                     /locus_tag="VLR27_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HSP02894.1"
                     /translation="MPQGPELLIILFVIVLLFGGAKLPKLARSLGRAQTEFKQGLSEG
                     TAPTSDDTDPATTA"
     gene            complement(1562..2941)
                     /locus_tag="VLR27_05305"
     CDS             complement(1562..2941)
                     /locus_tag="VLR27_05305"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HSP02895.1"
                     /translation="MTLTRRELLRNSALSGVALAAGGTFSGLALGGVAGATGPGRGPG
                     PGPGRTYGRLVPDPDGILDLPEGFRYEILAEGGTGHPDHFTTYDDGQKLAGDADGMAS
                     FAIGGNRTVLVTNHELTPSERAEMVPHTFAGQPVPTYDPAATGGTSNIVLDRDNRVVS
                     VYPSIAGTHNNCAGGPTPWGTWLTCEENESDIGVVPHGYVFEVDPEGKKTVATPYKAM
                     GRYKHEAAAVDPETSAVYLTEDHNDGLFYKFVPDDTSMQYGSLGNGGTLYAMKAGDLG
                     RLGEIKTVGRSVKVRWTEISNPDQQGLNGKDELADDAAVTRSRKLEGCWYGNRRIYFN
                     CADENASPEVAHHGQVWSLDPKTDVLTLVAYIPTTDPVFDRPDNVTVTPWGQLILCED
                     GDDDQYLILCDPRTGELSPFAHNAMGSSEFAGANFSPDGQTLFVNIMGPSTTFAITGP
                     WTSARPRRR"
     gene            complement(3038..5071)
                     /locus_tag="VLR27_05310"
     CDS             complement(3038..5071)
                     /locus_tag="VLR27_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSP02896.1"
                     /translation="MSRPDAFFREVVESARDIVVVMARDGRLRYVNGAVRSVLGYDTS
                     WQAATDVFDLVHEADRSRVMEDFVASVDSTSPGVPVTFRMRHRDGSWRHVEAAGVNLF
                     DHEAVGGFVVSLRDVTDRVEAETRHERASARYRRALDSSPQATAIHQDGDIVYANDAA
                     ARLLGADDTEELVGRPVLDFVPMHVAEMIRDRIALVLEGQEADLVEEQFRRVDGALVD
                     VEAAAVPIEWLGRPAVQVVARDLTERNRARSELEMQATHDPLTGRPNRVLLARRLQLA
                     ESRRRQTGKRYAVVFTDLDKFKVLNDGLGHSAGDVVLRAVADRLAGVARTHDTVARFG
                     GDEFVLVLEDLEADADVVEIVQRFADALREPVAVGTNLVRVGMSGGIVVVDDDLGDRD
                     VLSEADAALYRAKQIGRGRFEIFDRDLRHEVQVRYRREAELHEAFERDEFIAHFQVEV
                     DLRTGAPVGAEALCRWVHPEEGVLLPGDFLHVVEEIGAIHELGDLMRERALAALARTR
                     AGGRFTDCWVGVNVAAAELLGPGFVDRVMQSLERHDLPADALCLELTESSLASDPDSA
                     VRIVGELRAHGVPFAVDDFGTGYASLSYLDRFAPEFVKVDRVFIDGMLTDDRRLALVR
                     SAIEIAHTFGAVTVAEGVESEAQRQRLRELGCDLIQGHLIGRPGPELEAVWSG"
     gene            5211..5579
                     /gene="trxA"
                     /locus_tag="VLR27_05315"
     CDS             5211..5579
                     /gene="trxA"
                     /locus_tag="VLR27_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HSP02897.1"
                     /translation="MSTVALTEADFESTITDNDIVLVDWWASWCGPCRAFAPVYEKAA
                     GVHEDIVFAKVDTEAAPHLSAAAGIQSIPTLMAFREGVLVFAQPGALPAAALEDVIAQ
                     VRALDMEEVHRAVAERADVA"
     gene            5587..>5875
                     /locus_tag="VLR27_05320"
     CDS             5587..>5875
                     /locus_tag="VLR27_05320"
                     /inference="COORDINATES: protein motif:HMM:NF016910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02898.1"
                     /translation="MTDAIDDAVTTAPGQLERRGRWWLAVSFLFCPCHLPLTLGLLAS
                     VLGGTALGGLLRDHGWVAGIVIAATWLAGTARGLTWIRRAERGELTCAVRPP"
BASE COUNT          861 a         2013 c         2119 g          882 t
ORIGIN      
        1 atcaagggga tttcctccat gcgaggggaa cggtttggtt tctgttgtcg tcggcgaccg
       61 taggaagccg accttgacgc caggccgagc ccaggtggac cggaggtgaa ctgatcggag
      121 cgtctgggcg aactggcagc cggcgtgtcc ggatgtgccc gatcggcgcc caccgccggg
      181 ttggatccat cgctcaccga tcgtgggatc ggcgggtgag gagcatgccg atgaggatcg
      241 atgcctcgta gaagaggtac atcgggatcg agaggatgcc gagcgtgtac gggtcgcccg
      301 acggggtgat cacggcgacg acgacgacga tcccgaccag ggcgtagcgc cgggcactgc
      361 gcaggctcga tgcggtgacg acgccggcga gctggaggaa gaccaggacg atcgggaact
      421 cgaagccgac gccgaaggcc agcatcatgt aggtgatgag ctcgaagtag ggggcgggtc
      481 ggaactgctg ctgcaggccc tcgccgccca cctcggtgag gaactcgagc gcgggggtga
      541 gcgtgtggaa cgcgagcgca gcgccgagca cgaagagcac gagcgcggat cccacgaacg
      601 ggtaggccca gcgcttctcg ttcgcatgga gcccggggac gacgaaccgc cagacctgcc
      661 acaacaggac cggcatggcc agcgcgatgc ccccgtagcc ggcgatcttc agccggatgg
      721 agaacgcctc gagcggctcg gtctgcagga gccggcagtc gtcgagcgtg gcgccctgga
      781 ggttgcagta cgggtcgacc aggacgtcga agatcgccgg gtacaggaac cagcacacca
      841 cggccccgac ggcgaccgcc gctgccgact tgatgatccg gtcccgcagc tcggacaggt
      901 ggtcgagcac ggacagctcc gtaggccggg acgcgtccgg cgcggggctg tgtccgctgt
      961 gccgtcgtcg tatcgcgagc acgggtgctc agtcgatccg gggctcggcg ccgggacgat
     1021 cgactgcgtc ctcggcgagc gcagcgttca catcgatgcg aaagctgtcg gacatccgcc
     1081 gcacctgtcc gagcagtgcg cccgcctgcc ggagggcagc cggcagcttg tccggcccga
     1141 gcacgaccag ggcgaccagg gcgatcgcca cgagctcgag gcctccgatg ttgaacacgg
     1201 tgaaccccgg tgccggccgt cggtcaggcc gtcgtggccg ggtcggtgtc gtcgctcgtc
     1261 ggagcggtgc cctcgctcag accttgcttg aactcggtct gtgcccttcc cagggagcgg
     1321 gccagcttgg gcagcttggc tccgccgaac agcaggacga tgacgaacag gatgatgagg
     1381 agctcggggc cttggggcat gcgggcgaag gtaggaggcg gcggttgcgg gtccctggcc
     1441 gtccggtgtg gcgccggtga acggcagcgg aagtcgcagc ggcggtgcgt aggtgcccgg
     1501 acgcgagtca ggccggccac ccgcggaacg ggcaaccggc ctggtcgatg gggggttcgc
     1561 ttcagcggcg ccgggggcgc gcgctggtcc acggtccggt gatggcgaac gtggtggacg
     1621 gccccatgat gttgacgaac agcgtctggc cgtccgggga gaagttggcg ccggcgaact
     1681 cgctggatcc catggcgttg tgggcgaacg gcgacagctc tccggtgcga gggtcgcaga
     1741 ggatgaggta ctggtcgtca tcgccgtcct cgcacaggat gagctggccc cacggggtca
     1801 cggtgacgtt gtccggccga tcgaagaccg ggtcggtcgt cgggatgtag gcgaccagcg
     1861 tgagcacgtc cgtcttcggg tccagcgacc agacctgccc gtggtgcgcg acctccgggc
     1921 tggcgttctc gtcggcgcag ttgaagtaga tccggcggtt cccgtaccag cagccctcga
     1981 gcttgcggga ccgggtgacg gcagcgtcgt ccgccagctc gtccttgccg ttgaggccct
     2041 gctggtcagg gttcgagatc tcggtccagc ggaccttcac cgaccggccc acggtcttga
     2101 tctcaccgag acggcccagg tcgccggcct tcatggcgta gagggtcccg ccgttgccga
     2161 gcgacccgta ctgcatggac gtgtcgtccg ggacgaactt gtagaacagg ccgtcgttgt
     2221 ggtcctcggt gaggtacacg gcgctggtct cggggtcgac agccgcagcc tcgtgcttgt
     2281 accgacccat ggccttgtac ggcgtggcca ccgtcttctt gccctcgggg tcgacctcga
     2341 agacgtagcc gtgcggcacg acaccgatgt cgctctcgtt ctcctcgcac gtcagccacg
     2401 tcccccacgg ggtggggccg ccggcgcagt tgttgtgggt cccggcgatg ctcgggtaca
     2461 cgctcaccac ccggttgtcc cggtcgagga cgatgttcga cgtcccgccc gtcgctgctg
     2521 ggtcataggt gggcacgggc tgcccggcga aggtgtgggg caccatctcg gcgcgctcgc
     2581 tgggcgtgag ctcgtggttc gtgaccagga cggtgcggtt gccgccgatg gcgaaggacg
     2641 ccatgccgtc ggcgtcaccg gcgagcttct ggccgtcgtc gtaggtggtg aagtggtcgg
     2701 ggtggccggt gccgccctcg gccaggatct cgtagcggaa gccttcgggc aggtcgagga
     2761 tgccgtcggg gtcgggaacg agccggccgt aggtgcggcc cggacccggg ccggggcccc
     2821 gcccggggcc ggtcgccccg gcgacaccgc cgagcgccag cccggagaag gtgccgcctg
     2881 cggccagtgc gacgcccgag agggcgctgt tgcggaggag ttcgcgtcgt gtgagtgtca
     2941 tgcggcggac cgtaagagtc ggacttttcc ggcaaccgtc cgcgaggcag cgcgagggtg
     3001 aagggtgggt gagcgttcgt cgtgggactg cggtcggtca accgctccac accgcttcca
     3061 gctccggccc gggtcgaccg atgaggtggc cctggatgag gtcgcagccc agctcccgca
     3121 accgttggcg ctgcgcctcg gactccacgc cctcggccac ggtgacggcg ccgaaggtgt
     3181 gggcgatctc gatggcggag cggacgaggg cgaggcggcg gtcgtcggtg agcatcccgt
     3241 cgatgaacac gcggtcgacc ttgacgaact ccggcgcgaa gcgatcgagg tacgagagcg
     3301 acgcgtagcc ggtgccgaag tcgtccaccg cgaaggggac gccgtgagcg cgcagctccc
     3361 cgacgatgcg gaccgcggag tccgggtccg aggccagcga agactcggtg agctccaagc
     3421 agagcgcgtc cgccggcagg tcgtgtcgct ccaggctctg catgacgcga tcgacgaacc
     3481 cggggccgag cagctcggcg gctgccacgt tcacccccac ccagcaatcc gtgaaccgcc
     3541 ctccggcccg ggtgcgcgcc agcgcggcga gggcacgctc acgcatgaga tcgccgagct
     3601 cgtggatcgc accgatctcc tcgaccacgt ggaggaagtc ccctggcagg agcacgccct
     3661 cctccgggtg cacccaccgg cacagcgcct cagcgcccac gggcgcgccg gtgcgcagat
     3721 cgacctcgac ctggaagtgg gcgatgaact cgtctcgctc gaaggcctcg tgcagttccg
     3781 cctcccgccg gtaccgcacc tggacctcgt ggcgcaggtc gcgatcgaag atctcgaacc
     3841 ggccccggcc gatctgcttg gcccggtaca gcgccgcgtc cgcctcgctc agcacgtcgc
     3901 ggtccccgag gtcgtcgtcc accacgacga tgcctccgct catcccgacg cgcacgaggt
     3961 tcgtcccgac cgccaccggc tcgcgcagcg cgtcggcgaa tcgctggacg atctccacca
     4021 cgtccgcgtc ggcctcgagg tcctccagca ccagcacgaa ctcgtcgccc ccgaagcggg
     4081 ccaccgtgtc gtgcgtgcgt gcgacgccgg cgaggcgatc ggcgactgct cgcaggacga
     4141 cgtcgccggc gctgtggccg aggccgtcgt tgagcacctt gaacttgtcc aggtcggtga
     4201 acaccacggc gtagcgcttg ccggtctggc gccggcgaga ctcggcgagc tgcagccggc
     4261 gcgccagcag cacgcggttc ggtcgcccgg tgagcgggtc gtgcgtggcc tgcatctcga
     4321 gctcggatcg agcccggttc cgctcggtga ggtcgcgcgc gaccacctgg acggcggggc
     4381 ggccgagcca ctcgatcggg acggcggcgg cctcgacgtc gacgagcgcc ccgtcgaccc
     4441 gtcggaactg ctcctcgacc agatccgcct cctggccctc gagcaccaag gcgatccggt
     4501 ctcggatcat ctcggcgacg tgcatcggca cgaagtccaa gaccgggcga ccgacgagct
     4561 cctcggtgtc gtccgcgccg aggagtcggg ccgcagcgtc gttggcgtag acgatgtcgc
     4621 cgtcctggtg gatcgccgtg gcctgcgggg agctgtcgag cgcccgccgg taccgcgccg
     4681 atgctcgctc gtgtcgggtc tcggcctcga cccggtcggt cacgtcgcgc aggctcacca
     4741 cgaacccgcc gacggcctcg tgatcgaaga ggttgacccc ggcggcctcg acgtggcgcc
     4801 acgacccgtc ccggtgtcgc atccggaacg tgaccggcac ccccggcgac gtggagtcga
     4861 ccgaggcgac gaagtcctcc atcacccgcg agcggtcggc ctcgtggacc aggtcgaaca
     4921 cgtcggtcgc cgcctgccac gaggtgtcgt atcccagcac cgaccggact gcaccgttca
     4981 cgtaccgcag ccggccgtcg cgggccatca cgaccacgat gtcccgcgca ctctcgacca
     5041 cctctcggaa gaacgcatcc ggacggctca cgagcaggtc ctcggtgtcg gcggcggtcg
     5101 gccgtgtcta ccagacaccc tccctggccg ccccggcacc acgtcctcgg aataccccag
     5161 ggggtaccga cgttggaagg agggaagccc atccaggagg agagattccc gtgtccaccg
     5221 tcgcactcac cgaagccgac ttcgaatcca ccatcaccga caacgacatc gtgctcgtgg
     5281 actggtgggc gtcgtggtgc gggccctgcc gcgccttcgc ccccgtgtac gagaaggctg
     5341 ccggcgtgca cgaggacatc gtcttcgcca aggtcgacac cgaggctgcg ccccacctct
     5401 ctgccgccgc cggcatccag tcgatcccca cgctcatggc gttccgcgag ggcgtcctcg
     5461 tgttcgccca gcccggtgcg ctccccgccg ctgcgctgga ggacgtgatc gcccaggtgc
     5521 gtgcactcga catggaagag gtccaccgcg ctgtcgccga gcgcgccgat gtcgcatgat
     5581 caggtggtga ccgacgccat cgacgacgcc gtcaccaccg ccccgggcca gctcgagcga
     5641 cgggggcggt ggtggctggc cgtctcgttc ctgttctgcc cctgccacct gccgctcacc
     5701 ctcgggctgc tcgcctccgt gctcggcggc accgcgctcg gcggcctgct ccgggatcac
     5761 gggtgggtgg ccgggatcgt gatcgccgcc acctggctcg cgggcaccgc ccgtgggctc
     5821 acctggatcc ggcgagccga gcgcggcgag ctgacctgcg cggtgcggcc gcccg
//
LOCUS       DATISU010000129         8626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_797425, whole genome shotgun sequence.
ACCESSION   DATISU010000129 DATISU010000000
VERSION     DATISU010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797425"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VLR27_05325"
     CDS             <1..445
                     /locus_tag="VLR27_05325"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSP02899.1"
                     /translation="VRNREHHDTVASLGAHRVVLPEDFVDAGPYDGGLELVGAPSWPG
                     NIQALATWGRIMVIGVGAGPKTELSLVDLMQKRGTIRASTLRSRPLEEKADAAQAMVR
                     HVLPLVDAGALTVPVEATFPMAEAEAAFDRFAEGGKLGKIVLVAA"
     gene            442..1287
                     /locus_tag="VLR27_05330"
     CDS             442..1287
                     /locus_tag="VLR27_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02900.1"
                     /translation="MTAALVDTVRARKCWRTLEPYHGFVYFAPEAAEEYASLGIEGRT
                     GYFLSRSAAMGRVSAATVIATFYNFNPSLVRHAMDGVPEGIDVADVLAARYRAVDRVL
                     RARLGDAVASSEMARAAELAERATTGLVERCVGRPLAAAHAALPVPDEDHLRLWWAIT
                     ALREYRGDGHLVALVDAELSGLEALVVHAASGDVGADVLKATRAWPDIEWAEAVDALA
                     ARGLLRADGSFTDAGRDLHQQIEDRTDRLAAPAWALLTDDEGAELRDLVRPWSKALFA
                     GMSAG"
     gene            complement(1491..2582)
                     /locus_tag="VLR27_05335"
     CDS             complement(1491..2582)
                     /locus_tag="VLR27_05335"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HSP02901.1"
                     /translation="MRVLFDVVHPAHVHFYRHLHDLLRAEGHETLVVARDKDVTLDLL
                     DAYGMPHEWTGRSGSKSTLGHAAELVARDVGLTRWARRFRPDVVLTRNPAGVHAGRLV
                     GAWTVFDTVDGRAAGVHFQLGAPLAHVVTSPNWLDEDYGPRHRRYRGPKELAYLHPDR
                     FEPDPGVRAELGVPDGQPLFVLRFVSHDAVHDTRTVGLDTAQRAALVHHLQDLGHVVI
                     SSEEALPAELEPLRYHLGAERIHDLLAAADLFVGDSGSMAAEAGVLGTPALRLSSWVG
                     PVRGYLGRMERDYGHVRCFTPDNRADFDAALEEVVADLPGAKATARAAADRLVADCDD
                     VTAWYRTLLDELLARPEPRSRRRLLRGTP"
     gene            complement(2582..3529)
                     /locus_tag="VLR27_05340"
     CDS             complement(2582..3529)
                     /locus_tag="VLR27_05340"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HSP02902.1"
                     /translation="MAEDATGRFCAAVCRRIVYVVGKGRSGSTLLDDLLGTVPGTASL
                     GELRQLWSRGFTPGYRCACGELVAECPVWSRAVVDAIGSTSAPALAEVGALQDRVLSW
                     PHVPRLLARGPGDDDRALGEVMGRLYGSLADQLGADVLVDSSKWPLPPGAFGVVPGFE
                     AWVLHLVRDPRAVAHSYRRRAGGDGQPELPRFGALHTALSWTARNAAAELGRRSVPAH
                     RFRRVRYEDLAADPRRTLRSLGEWLGIDEADRAFVDDRTVQLGEAHLVGGNPRRLERG
                     ATDIRADREWAGATTSRSQAVVGALTAPLRARYGYVGSR"
     gene            3528..4652
                     /locus_tag="VLR27_05345"
     CDS             3528..4652
                     /locus_tag="VLR27_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP02903.1"
                     /translation="MDFSWTPELLALAEEASEVGRKATEGRWFKEDSWIAQPDDAFAK
                     ELAARGWIGMTWPTEVGGGGRAALERFVVYEALIAEGAPIASSWFADRQMGPTLLQFG
                     TPEQRERWLPDIIAAESMWCIGMSEPDAGSDVASLRTHAVRDGDEWVVNGQKIWNSGG
                     AQADWCYLIARTDPDAPKHKGLSEFIVDMTSPGITVQPIRDMTDDDHFCEIYFDDVRV
                     PAANMVGTENNSFKQVMRQMEHERGGIDRLVSNKRLYEDCLDFLRAHGGLDDENLRQE
                     IAEIESMYRIGRLLVLRETLQQAPQGFSAATKTIGTEFEVKVANFCGRVLGPHATLAD
                     EGLRRRVARNICYAPGYIIMGGTVEILRNILGERVLGLPR"
     gene            complement(4649..7297)
                     /locus_tag="VLR27_05350"
     CDS             complement(4649..7297)
                     /locus_tag="VLR27_05350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP02904.1"
                     /translation="MAQGTIDRERWGQDVVLADGSTVHIRPIDPSDGDALVAFHGRQS
                     RESVYFRYFSPRPHLSDDDIRHLTTVDGIDRMAFVAARDDEILGVARYDRYPDRPVAE
                     VAFFTDDQMGGKGIATLLLEYLAAYGREIGISRFEASVLPSNRRMVRVFQAAGYGASS
                     AFADGVIEVTFDIEPSEEAAQAMAERARRAERQAVVRLLEPRAVAVVGAGRNPRGIGH
                     RVLRSLLGGPFRGPVYPVHPEASAVAGVKAFRSVTELPDEIDVAVVCVPAAEANQVIE
                     DCGRARVAAAIVISAGFAETGPEGKERQEELLATARRFGVRILGPNCLGVINTDPEVR
                     FQATFAGIVPKPGRVGLLSQSGTLGAVILGHARDAGLGISSFVAVGNKADLSGNDLLL
                     YWLDDDRTDVVLLYLESFGNPRRFGRNVRQVAARKPVFAVRTGAVMDSLISEEQAEDW
                     LDDATIDALLRQTGVVRVPTVTSLLNAAIVAENQPVPAGNGVVVVGNSGGSASMAADA
                     CVNAGLRMVTLSESTLAAVDDVRLHGRRTTNPVDLPFDASPDDYRAVLDAVLVDPAVD
                     VALVVHAPYDPDEPDEVAAALDQAAAEHPASTIVSCIYGQRTTVTDGGVPMFAFPDDA
                     AYALGWVARYGVWRSAELSGEVLACPTSDEVVTLVERLLAEDERVELEPEAARGLLRA
                     GGVEVVPVTVVRSPADLETLEVQGPVALKAEAHDVGATTQRRGVALDLHDHDDVMAAA
                     ATIVEGMGEDAWPMVLQEMVEAGTDVRISIGTHPVVGPVVRLGPGGGAGRFAPSPRRV
                     LPLTDRGADELVDESGLHEVLDAESRRHVVDLVRRLCAIVDAAPEVTELTCDPVIVRP
                     DGADVIEVRATLAEVDVDDVPLVRRL"
     gene            7444..8502
                     /locus_tag="VLR27_05355"
     CDS             7444..8502
                     /locus_tag="VLR27_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02905.1"
                     /translation="MPTLRTTLAALASALVLAVPASALAATEGATIDKTGWWNRASSD
                     TPTPAGPVTVPPPPGIPEGALAVGRLGSEATAMAAVGIQPEEGPGATVESFTLTLRED
                     PDATGNQGTEEAAILACPITSFWAGGENGAWETQPEHDCEAASTAGVRADDGTWTFDL
                     AAIGALWFDTFGTIRADGVALVADADAEGSFQVVWQGGDAIDVELRAEPGPTGDDAFA
                     APPSFDPPADAGLSSGPGGGGSSIFAPPRVTTPPSIAPPPAVDTSPVETAADPDEGGD
                     DEVAGAPVVDDDPRGTRAGMLIGNLPPAGFLLVPVALATMFAMSYWLGPAGQPSTLDR
                     RGGVSRALALRARTAQER"
BASE COUNT         1178 a         3191 c         3158 g         1099 t
ORIGIN      
        1 cgtccgcaac cgggagcacc acgacaccgt ggcgtcgctc ggagcgcacc gcgtcgtgct
       61 cccggaggac ttcgtcgacg ccggccccta cgacggcggc ctcgagctgg tcggcgcccc
      121 gagctggccg ggcaacatcc aggcgctggc cacgtggggc cggatcatgg tcatcggcgt
      181 cggcgccggc ccgaagaccg agctgtccct ggtcgacctc atgcagaagc gcggcaccat
      241 ccgggcctcg accctgcgct cccgccccct cgaggagaag gccgacgcgg cccaggccat
      301 ggtccgccac gtcctgcccc tggtcgatgc cggtgcgctg acggtgccgg tggaggccac
      361 gttcccgatg gccgaggccg aggcggcctt cgaccggttc gccgagggcg gcaagctcgg
      421 caagatcgtg ctggtcgcag cgtgaccgcc gcgctggtcg acaccgttcg cgcccgcaag
      481 tgctggcgga cgctcgagcc gtaccacggc ttcgtctact tcgcccccga ggccgccgag
      541 gagtacgcct ccctcggcat cgagggccgc accggctact tcctgtcccg ctcggcagcg
      601 atgggccggg tgtcggcggc caccgtcatc gccaccttct acaacttcaa cccgtcgctg
      661 gtgcgccacg ccatggacgg cgtgcccgaa gggatcgacg tcgccgacgt cctcgccgcc
      721 cggtaccgcg ccgtcgatcg ggtcctgcgt gcccggctgg gcgacgccgt cgcctcgtcc
      781 gagatggcac gcgccgccga gctcgccgag cgggccacca ccggtctggt cgagcgctgc
      841 gtcggccgtc ccctcgccgc cgcccacgcc gccctgccgg tccccgacga ggaccacctc
      901 cgcctgtggt gggccatcac cgcgctgcgg gagtaccggg gcgacggcca cctggtcgcc
      961 ctggtcgacg ccgagctgtc cgggctcgag gcgctcgtcg tgcatgccgc gtcgggtgac
     1021 gtcggcgccg acgtgctcaa ggcgacccgg gcctggcccg acatcgagtg ggccgaggcg
     1081 gtcgacgcgc tcgccgctcg ggggctgctg cgagcggacg ggtccttcac cgatgccggg
     1141 cgcgacctgc accagcagat cgaggaccgc accgaccgcc tggccgcccc ggcgtgggcg
     1201 ctcctcaccg acgacgaggg cgccgagctg cgcgacctcg tgcgaccgtg gtccaaggcg
     1261 ctcttcgccg ggatgtccgc cggctgagac atcccgggct ccggcgccac gccattggag
     1321 cgccagagcc cggtgatcgt gatgtcgttg ctcgggttcc attacccccg tgtgccggtc
     1381 gtcacccctg gattttcccc gcattccgga catcgtccga tccggagctg gcagtgctgc
     1441 cggcacgtcc ggccgacgtt ctccagcgtt ccgtggtcgc tgccagttcc tcagggggtc
     1501 ccgcggagga gccgtcgccg gctgcggggc tcggggcggg ccaggagctc gtcgagcagg
     1561 gtccggtacc aggcggtgac gtcgtcgcag tcggcaacca gccggtcggc ggccgctcgg
     1621 gcggtggcct tggcgccggg gaggtcggcg accacctcct cgagcgcggc gtcgaagtcg
     1681 gcgcggttgt cgggggtgaa gcatcggacg tgcccgtagt cgcgctccat ccgtccgagg
     1741 tagccgcgca ccgggccgac ccacgacgac aggcgcagcg ccggcgtgcc gagcaccccg
     1801 gcctcggcgg ccatcgaccc ggagtcgccg acgaagagat cggcggcggc gagcaggtcg
     1861 tggatgcgct cggctccgag gtggtagcgg agcggctcga gctcggcggg cagggcctcc
     1921 tcgctcgaga tcacgacgtg acccagatcc tggaggtggt ggacgagggc ggcccgctgg
     1981 gcggtgtcga ggccgacggt gcgggtgtcg tgcaccgcgt cgtgggacac gaagcggagc
     2041 acgaacagcg gctgaccgtc cgggacgccg agctcggccc gcacgcccgg gtccggttcg
     2101 aagcggtccg ggtggaggta cgccagctcc ttgggccccc ggtaccgccg gtggcgcggc
     2161 ccgtagtcct cgtccagcca gttcggggag gtgacgacgt gggccaacgg cgcgccgagc
     2221 tggaagtgga cgccggcggc ccggccgtcg acggtgtcga acacggtcca ggccccgacg
     2281 aggcggccgg cgtggacacc ggcggggttg cgggtgagca cgacgtcggg ccggaagcgg
     2341 cgggcccacc gggtcaggcc gacgtcccgg gcgaccagct ccgccgcgtg gccgagcgtc
     2401 gacttcgagc ccgaccgacc ggtccactcg tgcggcatgc cgtaggcatc gagcaggtcg
     2461 agggtcacgt ccttgtcccg ggcgacgacg agcgtctcgt gcccctcggc gcggagcagg
     2521 tcgtggaggt ggcggtagaa gtggacgtgc gccgggtgga cgacgtcgaa gaggacccgc
     2581 atcagcgcga cccgacgtag ccgtagcggg cccgcagcgg agcggtgagg gcgcccacca
     2641 cggcctggct gcgggacgtg gtcgcgccgg cccactcccg gtcggcgcgg atgtcggtgg
     2701 cgccgcgttc cagccggcgc gggttaccgc cgacgaggtg ggcctcgccc agctgcacgg
     2761 tccggtcgtc gacgaaggcc cggtcggcct cgtcgatgcc cagccactcc cccagcgagc
     2821 gcagcgtccg gcgcgggtcg gcggccaggt cctcgtagcg cacgcggcgg aagcggtgcg
     2881 cggggaccga tcgccggccg agctcggccg cggcgttgcg ggcggtccag gacagcgcgg
     2941 tgtgcagcgc accgaagcgg ggcagctcgg gctggccgtc cccgccggcg cgccgccggt
     3001 agctgtgggc caccgctcgg gggtcacgga ccaggtgcag cacccatgct tcgaacccgg
     3061 gcacgacgcc gaaggccccg gggggcagtg gccacttcga ggagtcgacg agcacgtcgg
     3121 cgccgagctg gtcggccagc gacccgtaga ggcgccccat cacctcgccg agggcccggt
     3181 cgtcgtcgcc cggccctcga gcgagcagcc gggggacgtg cggccacgac agcacccggt
     3241 cctgcagggc accgacctcg gccagcgccg gcgcggacgt ggagccgatg gcgtcgacga
     3301 cggcacgcga ccagaccggg cactcggcga ccagctcgcc gcacgcgcac cggtagcccg
     3361 gcgtgaaccc gcgcgaccac agctgccgca gctcaccgag cgaggcggtt ccggggacgg
     3421 tgccgaggag gtcgtccagg agggtcgacc cgctccggcc cttcccgacg acgtagacga
     3481 tccggcgaca cacggcggcg cagaacctac ctgtagcgtc ctcggccatg gacttctcgt
     3541 ggacgccgga gctgctcgcc ctcgcggaag aggccagtga ggtgggccgc aaggccaccg
     3601 agggccgctg gttcaaggag gactcctgga tcgcccagcc cgacgacgcc ttcgccaagg
     3661 agctggcggc acggggctgg atcggcatga cctggcccac cgaggtcggc ggcggcggcc
     3721 gcgccgccct cgagcgcttc gtggtctacg aggccctcat cgccgagggc gcgcccatcg
     3781 ccagctcgtg gttcgcggac cgccagatgg gcccgacgct gctccagttc ggcacgcccg
     3841 agcagcgcga gcgctggctg ccggacatca tcgccgccga gtcgatgtgg tgcatcggca
     3901 tgtccgagcc cgacgccggc tccgacgtgg cgtccctgcg cacccacgcc gtgcgcgacg
     3961 gcgacgagtg ggtcgtcaac ggccagaaga tctggaactc gggcggtgcc caggccgact
     4021 ggtgctacct catcgcccgc acggaccccg acgccccgaa gcacaagggc ctgtcggagt
     4081 tcatcgtcga catgacctcg cccggcatca ccgtgcagcc gatccgcgac atgaccgacg
     4141 acgaccactt ctgcgagatc tacttcgacg acgttcgggt gccggcggcg aacatggtcg
     4201 gcacggagaa caactccttc aagcaggtca tgcgccagat ggagcacgag cgaggcggca
     4261 tcgaccggct cgtgtccaac aagcgcctgt acgaggactg cctcgacttc ctccgggccc
     4321 acggcggcct cgacgacgag aacctgcgcc aggagatcgc cgagatcgag tcgatgtacc
     4381 gcatcggccg cctgctcgtg ctgcgtgaga ccctgcagca ggccccgcag gggttctcgg
     4441 ccgccaccaa gaccatcggc accgagttcg aggtgaaggt cgccaacttc tgcggccggg
     4501 tcctcggccc ccacgccacc ctcgccgacg agggcctgcg ccggcgggtc gcccgcaaca
     4561 tctgctacgc ccccggctac atcatcatgg gcggcacggt ggagatcctg cgcaacatcc
     4621 tcggcgagcg ggtgctcggt ctgccccgct agaggcgacg gacgaggggg acgtcgtcga
     4681 cgtcgacctc ggcgagcgtg gcccgcacct cgatgacgtc ggccccgtcc gggcggacga
     4741 tcaccgggtc gcaggtgagc tcggtgacct ccggggcggc gtcgacgatg gcgcacaggc
     4801 gtcggacgag gtcgaccacg tggcggcggg actcggcgtc gaggacctcg tgcagcccgg
     4861 attcgtcgac gagctcgtcg gcgccacggt cggtgagcgg gagcacccgg cggggtgacg
     4921 gcgcgaaccg gccggcgcca ccgccgggcc cgaggcgcac gaccggcccg acgacggggt
     4981 gcgtgccgat gctgatgcgg acgtcggtgc ccgcctcgac catctcctgc aggaccatcg
     5041 gccaggcgtc ctcgcccatc ccctcgacga tggtggcggc ggcagccatc acgtcgtcgt
     5101 ggtcgtggag gtcgagcgcc acgccccggc gctgggtggt ggcgcccacg tcgtgggcct
     5161 cggccttgag ggcgacgggg ccctgcacct cgagcgtctc gaggtccgct gggctgcgca
     5221 ccacggtgac cgggaccacc tcgacgccgc cggcccgcag gagcccgcgt gcggcctccg
     5281 gctcgagctc gacgcgctcg tcctcggcga gcaggcgctc gaccagggtc acgacctcgt
     5341 ccgaggtcgg gcacgccagc acctcgccgg acagctcggc ggagcgccac accccgtagc
     5401 gcgccaccca gcccagggcg taggcggcgt cgtcggggaa ggcgaacatc ggcacgccgc
     5461 cgtcggtgac cgtggtccgc tgcccgtaga tgcaggacac gatggtgctg gccgggtgct
     5521 cggccgcggc ctggtcgagt gcggccgcca cctcatcggg ctcgtcgggg tcgtagggcg
     5581 cgtgcaccac gagggcgacg tccaccgccg gatcgacgag caccgcgtcg agcacggccc
     5641 ggtagtcgtc gggcgaggcg tcgaagggca ggtccaccgg gttggtggtc cggcggccgt
     5701 gcaggcggac gtcgtcgacg gcggcgaggg tcgactcgga cagcgtcacc atccggagcc
     5761 cggcgttcac gcacgcgtcg gcggccatcg acgcgctgcc cccgctgttg ccgacgacca
     5821 cgacgccgtt gccggccggc accggctggt tctcggccac gatcgccgcg ttgagcagcg
     5881 acgtcaccgt gggcacccgg accacaccgg tctggcgcag cagggcgtcg atcgtggcgt
     5941 cgtcgagcca gtcctcggcc tgctcctcgg agatcaggga gtccatcacc gcgcccgtcc
     6001 gcacggcgaa gaccggcttg cgggccgcga cctgacggac gttgcgaccg aagcggcggg
     6061 ggttgccgaa ggactcgagg tagagcagga ccacatcggt gcggtcgtcg tccagccagt
     6121 agaggagcag atcgttgccc gacaggtcgg ccttgttgcc cacggccacg aagctggata
     6181 tgccgaggcc ggcgtcgcgg gcgtggccga ggatcaccgc gccgagggtc cccgactggc
     6241 tgaggaggcc gacccgaccg ggcttgggga cgatgccggc gaacgtggcc tggaaccgga
     6301 cctcgggatc ggtgttgatg acgcccaggc agttggggcc gaggatgcgg accccgaacc
     6361 ggcgggccgt ggccagcagc tcctcctggc gctccttgcc ctccggtccg gtctcggcga
     6421 agccggcgga gatcacgatg gccgcagcga cccgggcccg cccgcagtcc tcgatgacct
     6481 ggttggcctc ggcggcgggc acgcacacga ccgcgacgtc gatctcgtcg gggagctcgg
     6541 tgacgctgcg gaacgccttc accccggcga ccgcgctggc ctcggggtgc accgggtaga
     6601 ccggcccgcg gaacggtcct ccgaggaggc tgcgcagcac gcggtgcccg atcccccgtg
     6661 ggttccggcc ggcaccgacc acggccacgg cgcggggctc gagcagccgg accaccgcct
     6721 gccgctcggc ccgacgagcc cgctcggcca tggcctgggc cgcctcctcg ctgggctcga
     6781 tgtcgaaggt gacctcgatg accccgtcgg cgaacgcgct cgacgcgccg tagcccgcag
     6841 cctggaacac ccggaccatc cgccggttgg acggcagcac gctggcctcg aagcggctga
     6901 tgccgatctc gcgcccgtag gcggcgaggt actcgagcag cagcgtggcg atgcccttgc
     6961 cgcccatctg gtcgtcggtg aagaaggcga cctcggcgac ggggcggtcc gggtagcggt
     7021 cgtagcgggc cacgccgagg atctcgtcgt cgcgtgcggc cacgaaggcc atccggtcga
     7081 tgccgtcgac cgtggtcagg tggcggatgt cgtcgtcgga caggtgcggc cgggggctga
     7141 agtaccggaa gtacaccgac tcccgggact ggcgcccgtg gaacgcgacc agcgcgtcgc
     7201 cgtcggaggg atcgatgggt cggatgtgca ccgtggaccc gtcggccagg acgacgtctt
     7261 ggccccatcg ctcgcggtcg atcgtgcctt gtgccactcc gccagcgtag gaccgcaggc
     7321 aggaggtgcc aggccgcgtg ccgaaacgtg cggtggccgc tcgattacca cccggtaacc
     7381 gcagcgaccg ggttcgcagc ccccaccggg tcggccgggg tcgcgtgctc cagggaacca
     7441 accatgccaa cgctccgcac caccctcgcc gcgctcgcgt cagcgctcgt cctcgccgta
     7501 ccggcgagtg cactcgccgc gaccgaaggc gccaccatcg acaagaccgg gtggtggaac
     7561 cgcgccagct cggacacccc gacgcccgcc ggcccggtga ccgtgccccc gccgcccggg
     7621 atccccgaag gtgcgctcgc cgtgggtcgc ctgggcagcg aggcgaccgc catggccgca
     7681 gtcggcatcc agcccgagga gggcccgggc gccacggtcg agtccttcac cctcaccctg
     7741 cgcgaggacc ccgacgcgac cgggaaccag gggacggagg aggcggcgat cctcgcctgc
     7801 cccatcacca gcttctgggc cggtggggag aacggtgcgt gggagaccca gcccgagcac
     7861 gactgcgagg cggccagcac cgccggcgtc cgagccgacg acggcacctg gaccttcgac
     7921 ctcgccgcca tcggcgcgct ctggttcgac accttcggga ccatccgggc cgacggcgtg
     7981 gcactggtgg ccgacgcgga cgccgagggt tccttccagg tcgtctggca gggcggtgac
     8041 gccatcgacg tcgagctccg tgccgaaccg ggccccaccg gcgatgacgc gttcgcagca
     8101 ccaccctcgt tcgatccacc tgcggacgcc ggcctgagca gcggacccgg gggcggcggc
     8161 tcgagcatct tcgccccgcc ccgcgtcacc accccgccct ccatcgcccc gccgccagcg
     8221 gtcgacacca gccccgtcga gaccgccgcc gaccccgatg agggcggcga cgacgaggtc
     8281 gccggggcac ccgtggtcga cgacgacccg cgaggcaccc gcgccggcat gctcatcggc
     8341 aacctccctc cggccggctt cctgctcgtg cccgtcgccc tggcgaccat gttcgccatg
     8401 tcctactggc tcggccccgc cggccagccg tccaccctcg accgtcgagg cggggtgagc
     8461 cgagccctcg cgctccgcgc ccgcaccgca caggagcgct gacaaccgtg aacccggtcc
     8521 gacgtaccca ccgccgatcg gccaccgtgg ccgtgctcgt cgcgctgctg ctcatcgccg
     8581 cggcgtgcgg gcagaagacc gacgtcgcca gcgtctccgc cggcgc
//
LOCUS       DATISU010000130         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_704635, whole genome shotgun sequence.
ACCESSION   DATISU010000130 DATISU010000000
VERSION     DATISU010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704635"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1046
                     /locus_tag="VLR27_05360"
     CDS             108..1046
                     /locus_tag="VLR27_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSP02906.1"
                     /translation="MTTTIDPPAPPPKGQGYPQRPVQRETGRIEWGQIATVARTDLKQ
                     LIQAKDFWGPMVGLGAIFFCVVPTILLFSLTSIAGVDAVEQISQTLEVLPSRAQASIE
                     AATSGGDGGSGSTPAGQASYALAVFLFAPVAVVVPLTISTAVGAATIVGERERGTGEF
                     LAHSPAGVREIYLGKLLASLIPGYVTTVVGFGIYSLIVNLIVGPEVGGWFFPTTQWWI
                     LMAWVVPPFLALTLSLVLRLSARVKSTAAAQQASGLVSLPLIMVAYSQSTGALFGGAR
                     VTWIIGGLAWLLAILGLRRGMRAVSRARLLGVADEQ"
     gene            complement(1069..2715)
                     /locus_tag="VLR27_05365"
     CDS             complement(1069..2715)
                     /locus_tag="VLR27_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HSP02907.1"
                     /translation="MSAQIVARNLSFAHGPEVVLADVSLTVASGHRIGVVGPNGVGKS
                     TLLRLLTGELEPERGTVARVPADARVGHLTQELDVRSGETIADVIARRTGADMAQREL
                     DASMRAMAAGDADAADRYSAALETWLHLGVADLEATMAETLERVGLGADRAEQPAATL
                     SGGERARVNLASVLLAGFDLLALDEPTNDLDLAGLALLEDFLVHSPAGFVLISHDRTF
                     LERVVTSVVEIDEHARTATRYDGGWQAYLDARTAARRHAEEAFAGYVETRDNLRGQAQ
                     QTREWSAKGIGKVKRSGETDKFIRHFNKASSEKLAGKAARAEKALERLDVVDKPWEGW
                     DLRLDFATTGRSGDEVVALRDVVVDHGAAFTLGPVTLDIGYGERVALVGPNGSGKTTL
                     LRTMLGDIEPTSGTVRVGPSVVVGTLDQSRGRFAGDESLVDAFVAETGIRADEARSQL
                     AKLGLGPEHVSRPTSRLSPGERTRAVLAAFALVGVNLLVLDEPTNHLDLPAIEQLEAA
                     LAGYPGTLLLVTHDRRLLETVTLTRRVELADGTVVADDPL"
BASE COUNT          374 a         1070 c         1022 g          412 t
ORIGIN      
        1 gccggcgccc gggtgacccg ggtcgaggcc gagcgccact cgctcgagga cctctacttc
       61 gccgtccgcc gcgacagcgg cgagaaccgc atcggcgagg tggtccagtg accaccacca
      121 tcgacccgcc ggcgccgccg ccgaagggcc aggggtaccc gcagcgcccg gtgcagcgcg
      181 agaccggccg gatcgagtgg ggccagatcg ccaccgtcgc ccgcaccgac ctcaagcagc
      241 tcatccaggc caaggatttc tggggcccga tggtgggcct cggcgccatc ttcttctgcg
      301 tcgtcccgac catcctgctg ttcagcctca cctcgatcgc cggcgtggac gccgtcgagc
      361 agatcagcca gaccctcgag gtcctgccga gccgagccca ggcgtcgatc gaggccgcca
      421 cctccggggg cgacggcggc agcgggagca caccggcggg ccaggcctcc tacgcgctgg
      481 cggtcttcct gttcgccccg gtcgcggtcg tggtcccgct caccatctcc accgcggtgg
      541 gggccgccac catcgtcggc gagcgcgagc gcggcaccgg cgagttcctg gcccactccc
      601 ccgccggcgt gcgcgagatc tacctcggca agctcctcgc ctcgctcatc cccggctacg
      661 tcaccaccgt cgtcggcttc ggcatctact ccctcatcgt caacctcatc gtggggccgg
      721 aggtgggcgg ctggttcttc ccgaccaccc agtggtggat cctgatggcc tgggtggtgc
      781 ccccgttcct cgccctcacg ctctcgctgg tgctgcgcct gtcggcccgg gtgaagtcca
      841 ccgccgctgc ccagcaggca tcgggcctgg tcagcctccc gctgatcatg gtcgcctact
      901 cccagtccac cggcgctctg ttcggcggcg cccgggtcac ctggatcatc ggcggcctgg
      961 cctggctgct cgccatcctc ggcctgcgcc gcggcatgcg ggccgtctcc cgggctcgtc
     1021 tcctcggcgt cgccgacgag cagtaggccg ccggccgagc cttccagctc agagcgggtc
     1081 gtcggcgacg accgtgccgt cggccagctc gacccgccgg gtgagggtga ccgtctcgag
     1141 cagccggcgg tcgtgcgtga cgagcagcag ggtgcccggg tagcccgcca gggcggcctc
     1201 gagctgttcg atggcgggca ggtccaggtg gttggtcggc tcgtcgagga cgagcaggtt
     1261 caccccgacg agcgcgaacg ccgcgagcac agcccgggtc cgctcaccgg gagacagccg
     1321 gctggtgggc cgggacacgt gctccggccc gaggccgagc ttggccagct gggagcgagc
     1381 ctcgtcggcg cggatcccgg tctcggccac gaaagcgtcg acgagggact catcgccggc
     1441 gaagcgaccg cgcgactggt ccagcgtgcc gacgaccacc gacggaccga cccgcaccgt
     1501 gccgctggtc ggttcgatgt cgccgagcat cgtgcgcagg agcgtggtct tgcccgaccc
     1561 gttcggcccg acgagggcga cccgctcccc gtagccgatg tccagggtga ccggcccgag
     1621 cgtgaacgcc gcaccgtgat cgacgacgac gtcgcgcagc gcgaccacct catcgcctga
     1681 ccgtcccgtc gtggcgaagt ccaagcggag gtcccagccc tcccacggct tgtcgaccac
     1741 gtcgagccgc tcgagcgcct tctccgcccg ggccgccttg ccggccagct tctccgacga
     1801 cgccttgttg aagtggcgga tgaacttgtc ggtctcaccc gaccgcttca ccttgccgat
     1861 ccccttcgcc gaccactccc gggtctgctg ggcctggccc cggaggttgt cccgggtctc
     1921 cacgtagccg gcgaaggcct cctcggcgtg gcgccgggcg gcggtgcgag catcgaggta
     1981 ggcctgccag ccgccgtcgt agcgggtggc ggtgcgggcg tgctcgtcga tctccaccac
     2041 gctcgtgacc acccgctcga ggaacgtccg gtcgtgggag atcaggacga agccggcggg
     2101 cgagtgcacg aggaagtcct cgagcagggc caggccggcc aggtcgaggt cgttcgtcgg
     2161 ctcgtcgagg gccaggaggt cgaacccggc gagcaggacc gacgccaggt tcacgcgggc
     2221 tcgctccccg ccggacagcg tcgccgccgg ctgctcggcc cgatccgcac cgaggccgac
     2281 ccgttcgagg gtctcggcca tcgtggcctc gaggtcggcg accccgaggt gcaaccaggt
     2341 ctccagcgcg gcgctgtagc gatcggcggc atcggcgtca ccggcggcca tggcccgcat
     2401 cgacgcgtcg agctctcgtt gggccatgtc ggcgccggta cggcgggcga tgacgtcggc
     2461 gatcgtctcg cccgagcgca catcgagctc ctgggtgagg tgccccaccc ttgcgtcggc
     2521 ggggacacgg gcgaccgtgc cgcgctcggg ctcgagctcg ccggtgagca gccgcagcag
     2581 ggtggacttg cccaccccgt tcgggccgac cacgccgatg cggtggccgc tggcgacggt
     2641 gagcgacacg tcggcgagca cgacctcggg gccgtgggcg aaggacaggt ttcgggcgac
     2701 gatctgggcg gacactcagg ccctggcggc ctcgacggcg cgaaccagct cctcggtggg
     2761 gcggaacgtg agcccggcga tggcccggtg ggagtaggcc accttgccgg cggcgtcgat
     2821 cacgaacacc gagcgccggt agaagccgag cgggccgagg atgccgtagg cctccccg
//
LOCUS       DATISU010000131         1860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_6131, whole genome shotgun sequence.
ACCESSION   DATISU010000131 DATISU010000000
VERSION     DATISU010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6131"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VLR27_05370"
     CDS             <1..823
                     /locus_tag="VLR27_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02908.1"
                     /translation="ERATLPPPDPTATTQPEETTTTVDHSRTVLQAVAGQTTTTLAET
                     GDAVLRGSVSGPAGPVPDAVVRVERLVGDAVQVFEVRTGDVGGWVLEGIPGGRMRVRA
                     FAPPTLTMLEPEVFFLTESEPRDLQLVVREHQGIVVLSDVTPSAPTVGSAVSLAVRVM
                     ERVVGDDGVARTRPLPGQALQVRSSGWVFIDGPGATDGDGTAVFTYRCEQAATVTAAA
                     VVLVGAEEQTFPLDVPSCGPRPTTTTTTTTTEPDDEVDPDEETTSTSAPTTTEDD"
     gene            825..1361
                     /locus_tag="VLR27_05375"
     CDS             825..1361
                     /locus_tag="VLR27_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HSP02909.1"
                     /translation="MASHYDELGVAPAASPAEIRAAYVALARRHHPDRMGSASEPDRA
                     EAAARMARVNAAWTVLSDPDRRAAYDAGLAGPSATTSGAHVRDPDSSFQPFDTSEDVD
                     PRLFDDTPTGAPSIPRAFALAPATLAVVGVTMILGGAFLGYGGMLIAGFFVLALAGLS
                     FLALPFVALINASRNDRE"
     gene            1399..1710
                     /locus_tag="VLR27_05380"
     CDS             1399..1710
                     /locus_tag="VLR27_05380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HSP02910.1"
                     /translation="MSQLTVIARIQAQEGKRDEIVAAFQEYFPQVESEDGTRVYAIST
                     DTGDETSVWVYEMYTDNDALKAHGGSDAFKAFGGKIGGLLAGAPELHICTPVQAKGLP
                     G"
BASE COUNT          255 a          717 c          649 g          239 t
ORIGIN      
        1 ggagcgggcc acgctgccgc caccggaccc caccgccacc acccagccgg aggagaccac
       61 gaccacggtc gaccactccc gcaccgtcct gcaggcggtg gccggtcaga cgaccacgac
      121 gctggccgag accggcgacg ccgtcctccg cggctcggtc tccgggccgg ccgggccggt
      181 tccggatgcc gtcgtgcggg tcgagcgcct ggtcggcgat gcggtccagg tcttcgaggt
      241 ccgcacaggt gacgtcggtg gctgggtgct cgagggcatc ccgggcggac ggatgcgcgt
      301 ccgagcgttc gccccgccga cgctgacgat gctcgagccc gaggtcttct tcctcaccga
      361 gtccgagccc cgcgacctgc agctggtcgt ccgcgagcac cagggcatcg tcgtgctctc
      421 cgacgtcacg ccgtcggcgc cgacggtggg cagcgcggtc agcctcgcgg tgcgggtgat
      481 ggagcgggtg gtcggcgacg acggcgtggc tcgcacccgc ccgctgccgg gtcaggcgct
      541 gcaggtccgc tcgtccggct gggtgttcat cgacgggccg ggggccaccg acggcgatgg
      601 cacggccgtg ttcacctacc gctgcgagca ggccgcgacg gtgacggcgg ccgcagtcgt
      661 gctcgtcggc gccgaggagc agacgttccc gctcgacgtc ccctcgtgcg gtccgcgacc
      721 gacgaccacg accaccacga ccacgacgga gcccgacgac gaggtggatc cggacgagga
      781 gacgacctcc acgtccgcgc cgaccacgac cgaggacgac tgacgtggcg tcgcactacg
      841 acgagctcgg cgtcgcgccc gcggcctcgc ccgccgagat ccgggctgcc tacgtcgccc
      901 tggctcgacg ccaccacccg gaccgaatgg gcagtgcgtc ggagccggac cgcgccgagg
      961 ccgcggcccg catggcccgg gtgaacgcgg cgtggacggt gctgtccgac cccgatcggc
     1021 gggcggcgta cgacgccggc ctggccggtc cgtcggcgac gaccagcggc gcccacgtgc
     1081 gcgatcccga cagctcgttc cagcccttcg acacctccga ggacgtcgat ccccggctgt
     1141 tcgacgacac gcccacgggc gcccccagca tcccccgggc cttcgccctc gccccagcaa
     1201 ccctggccgt cgtcggcgtg accatgatcc tcggcggggc gttcctcggc tacggcggga
     1261 tgctcatcgc cggcttcttc gtgctcgcgc tcgccgggct gagcttcctc gccctgccct
     1321 tcgtggccct gatcaacgcc agccggaacg accgggagtg atcatcgcag gcgaggccgt
     1381 cgggtagcgt cgccgcccat gtcgcagctc accgtcatcg cccgcatcca ggcccaggaa
     1441 ggcaagcgtg acgagatcgt cgccgccttc caggagtact tcccgcaggt ggagtccgag
     1501 gacggcaccc gcgtgtacgc catcagcacc gacaccggcg acgagacctc ggtgtgggtc
     1561 tacgagatgt acacggacaa cgatgcgctg aaggcccacg gcggcagcga cgcgttcaag
     1621 gccttcggcg gcaagatcgg cggcctgctc gccggcgccc ccgagctgca catctgcacc
     1681 ccggtgcagg ccaagggcct ccccggctga tcacgggcag cagccggcca tggcgaccta
     1741 cctcgacggg atcctgctcg cccaccggct gaccgctgcc ggcgacgacc gggacgaggg
     1801 cgagctccgg cgtgccgtcg agggcgccga gcccacccgc ggcttcgccc aggccctccg
//
LOCUS       DATISU010000132         6522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_378941, whole genome shotgun sequence.
ACCESSION   DATISU010000132 DATISU010000000
VERSION     DATISU010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378941"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VLR27_05385"
     CDS             <1..722
                     /locus_tag="VLR27_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSP02911.1"
                     /translation="HTQTAGVSLTAQQPRNNIARVATQALSAALGGTQSLHTDSYDEA
                     LALPTEEAARIALRTQQIVAHETGVANVADPLGGAPYVEWMTDEMERQAEEVFAHLDR
                     LGDGSILEGVYAGIEDGYFVSEIAEASYRFQREVNSGQRIIVGVNAFTEGNEDSEQNL
                     LRIDPGVEDLQLKRLATVRSDRDDTAVDAALARVRADAADPATNLMPALLDAVSAYAT
                     LEEVVAALESEFGRYTEKAVI"
     gene            722..1129
                     /locus_tag="VLR27_05390"
     CDS             722..1129
                     /locus_tag="VLR27_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HSP02912.1"
                     /translation="MADPQIRIVLAKLGLDGHDRGLRVVTRMLRDAGFEVVYLGLRQT
                     TEMVVSAAIQEDADAVGLSLHNASHMTLAPRMVQALRDNGLDIPVIVGGIIPDEDMQP
                     LRDAGVAAILGPGASASDVAQAVRDAVAGSAAV"
     gene            complement(1137..2687)
                     /locus_tag="VLR27_05395"
     CDS             complement(1137..2687)
                     /locus_tag="VLR27_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02913.1"
                     /translation="MSAARLPEPADLRARRFALLVVAGLLAAAGVAYELSLLLLGTVT
                     VGATERANAVVLGAAMAGMGAGAAFGGRLAGRPVRAFVGVEVVLAILGAAAAPALYWL
                     WASLDTFWGPLLVVAFAIGACIGAEMPLLAALNDQLARQRAASVVAKLTAADYLGAFL
                     GAIAFAFAVRPVLGLVGATMAIAAVNLLLAAAVPVVMGVPWRQGALVVPGALVLAAMV
                     VLAPTVVDDGRQRLYRDPILAVDDRGRSDIVVTERRHRDGRRDTRLFLDGDLQLSSVD
                     SYRYHEALVHPAVAAGPDRDLDVLVLGGGDCAAVTEALRHDQVESVTLVELDPRVTDA
                     VDDVEGFDELSAGCDDARTEVITADAFEWIRRTDRSFDVLVADFPDPDTAALGRLYSV
                     ELFVAARSVLAPDGSLSVQCGSPWFAQAAYWTCAATLEEAGWSSTPYLADVPSFGTWG
                     FHLAVPDGPAPLVTATEPPGARYWDRSVAAGSMVLPPDLVVERDQEPTTVLEPRIVDA
                     HQRAWFDY"
     gene            complement(2707..3150)
                     /locus_tag="VLR27_05400"
     CDS             complement(2707..3150)
                     /locus_tag="VLR27_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HSP02914.1"
                     /translation="MTTMLLAATTDGVLDDVGAAAAWTVASFVLFAVGFVIIDVLTPG
                     HLRTQIRDNINAAILVSAKMLAVAAIVFVSVWTAPDELGDGIVYAASQALWSLAVSVL
                     AFLGLDWLIPGGIRNVVNEPGFSPRVLVAGAAEVAVALTAAVALS"
     gene            complement(3199..3873)
                     /locus_tag="VLR27_05405"
     CDS             complement(3199..3873)
                     /locus_tag="VLR27_05405"
                     /inference="COORDINATES: protein motif:HMM:NF025408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4247 domain-containing protein"
                     /protein_id="HSP02915.1"
                     /translation="MSDVPGGSGQPDPWDAWRGWSAGSGDQPEDEGAWDAWKRWEYTP
                     RARPPMANGFGCTGCGSTGIGMIIAIVIFIMLISSFIGSIGASGDLSADDLIASIDAL
                     PVTGPRDQRPPDTGAGETTTFPAPGFPPAAVGDEIQAVRAPSQRDSSGESIFLLYEEA
                     TVAITPVADGSEVVVFEDNRAATRRFPTILLLPGWSGTLDDFDTRARSRGGIGGFRGG
                     GGGFGK"
     gene            complement(3870..4382)
                     /locus_tag="VLR27_05410"
     CDS             complement(3870..4382)
                     /locus_tag="VLR27_05410"
                     /inference="COORDINATES: protein motif:HMM:NF022389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2617 family protein"
                     /protein_id="HSP02916.1"
                     /translation="MHLELDSRPGTADVSADGLALRFHGTPGLDVVGTLFGTLEALGL
                     AVVVEVLAASHRVHVLDDDGVVATEQVACVDPAPDEPLPEHASHESPAHRLDFASEHP
                     DLDGSALGVLAAELRSGADDDATLVVSFPGHRDALTSIRVLADGWETWHLYPGERPHA
                     VRTRTHVAAR"
     gene            4457..5035
                     /locus_tag="VLR27_05415"
     CDS             4457..5035
                     /locus_tag="VLR27_05415"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HSP02917.1"
                     /translation="MFDPRADLSPGRRVRIDGRPGSVRGLIDLEEEGDRWLEALVQLD
                     EGLAVWLSVEWMPTPVATVWLPVRPTEVDGGPGEKQVRLGGTSFSFYENGTASYRATG
                     ETGTEPAGRVDYVDYVADDSRRVSFERYGEAGAGRRSTMLTGTCPQCGAPWQPTSTGH
                     CTSCGVNPTVDVGAWGTWEVSIGIPATNVTAA"
     gene            complement(5051..6250)
                     /locus_tag="VLR27_05420"
     CDS             complement(5051..6250)
                     /locus_tag="VLR27_05420"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSP02918.1"
                     /translation="MTISGHVADGFEPVRDAFAANFETHGELGAGVAVYVEGELVVDL
                     VGGTADPTSDADETPYGTDALQLVFSTTKGAAAVCANLLAQRGELDPDALVTDVWPEY
                     GQAGKEGTTIAMLLDHQAGVPVVDDPPSLEEVLQVTPIVEALAAQAPLWEPGTAHGYH
                     ALTYGWLVGEVVRRIDGRPIGQFFAEEVAGPLGLDFWIGLPDDQQDRVVPLVPSRPSA
                     DDFDMASLDPEILPLLQDMATAFLDPNSTTNRALMLDGTFLLGGGRLAWNLPEVRASQ
                     IPAANGVTNARSLAKMYAACVGEVDGFRLLDDDTLQRATKEHSGGRDRVLIMPTRFGL
                     GFMLTSSFSPLLGERSFGHPGAGGSLGFGDPDHGVGFGYVMSKMSSGLSNDPRSRGLI
                     EALRGCL"
     gene            6274..>6522
                     /locus_tag="VLR27_05425"
     CDS             6274..>6522
                     /locus_tag="VLR27_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HSP02919.1"
                     /translation="MAHVEDPPGVDLEALRAWMDGEGLGSGPLEDVEQLAGGTQNVLV
                     RFTRSGRGHVLRRPPLHKRANSDETMRREARVLAALAGS"
BASE COUNT          964 a         2397 c         2373 g          788 t
ORIGIN      
        1 tccacaccca gaccgccggc gtgtccctca ccgcccagca gccccgcaac aacatcgccc
       61 gcgtcgccac ccaggccctc tccgccgcgc tgggcggcac ccagtcgctg cacaccgact
      121 cctacgacga ggccctcgcc cttcccaccg aggaagccgc ccgcatcgcc ctgcgcaccc
      181 agcagatcgt cgcccacgag accggcgtgg ccaacgtggc cgaccccctc ggcggcgctc
      241 cctacgtgga gtggatgacc gacgagatgg agcgccaggc cgaggaggtc ttcgcccacc
      301 tcgaccgcct cggcgacggc tccatcctcg agggcgtcta cgccggcatc gaagacggct
      361 acttcgtgtc cgagatcgcc gaggcctcct accggttcca gcgcgaggtc aacagcggcc
      421 agcgcatcat cgtgggcgtc aacgccttca ccgagggcaa cgaggactcc gagcagaacc
      481 tgctccgcat cgaccccggc gtcgaggacc tccagctcaa gcgcctcgcc accgtgaggt
      541 ccgaccgcga cgacaccgcc gtcgacgccg ccctcgcccg ggtccgcgcc gacgccgccg
      601 accccgccac caacctcatg cccgccctgc tcgacgccgt gtccgcctac gccaccctcg
      661 aagaggtcgt cgccgccctc gagtccgagt tcggccgcta caccgagaag gcagtgatct
      721 gatggccgac ccccagatcc gcatcgtgct ggccaagctc ggcctcgacg gccacgaccg
      781 gggcctgcgc gtcgtcaccc gcatgctccg cgacgccggc ttcgaggtcg tctacctcgg
      841 cctgcgccag accaccgaga tggtcgtgtc cgccgccatc caggaggacg ccgacgctgt
      901 cgggctctcc ctccacaacg ccagccacat gaccctcgcc ccccgcatgg tccaggccct
      961 gcgcgacaac ggcctcgaca tccccgtcat cgtcggcggc atcatccccg acgaagacat
     1021 gcaaccgctc cgcgacgccg gcgtggccgc catcctcggc cccggtgcgt ctgcatcgga
     1081 cgtcgcccag gctgtgcgcg acgcggtggc gggcagcgcc gccgtctgag cgcggctcag
     1141 tagtcgaacc acgcccgctg gtgggcgtcc acgatgcgcg gctcgagcac cgtggtcggc
     1201 tcctggtccc gctcgacgac gaggtccggc ggcagcacca tcgaaccggc cgccaccgat
     1261 cggtcccagt agcgggcgcc gggtggctcg gtcgcggtga cgagtggtgc gggtccgtcc
     1321 ggcacggcca ggtggaagcc ccacgtcccg aacgacggca cgtcggccag gtacggcgtg
     1381 gacgaccagc cggcctcctc gagggtggcc gcacaggtcc agtacgccgc ctgggcgaac
     1441 cagggtgagc cgcactggac gctgagggac ccgtccggcg ccagcaccga gcgagccgcg
     1501 acgaacagct ccacgctgta gaggcggccg agcgctgcgg tgtcggggtc ggggaagtcg
     1561 gccacgagca cgtcgaagct gcggtcggtg cgccggatcc actcgaacgc atcggcggtg
     1621 atgacctcgg tgcgggcgtc gtcgcagccg gccgacagct cgtcgaaccc ctcgacgtcg
     1681 tcgacggcat ccgtgacccg gggatcgagc tccacgagcg tcaccgactc gacctggtcg
     1741 tggcgcagcg cctcggtgac cgcggcgcag tcccctccgc cgagcaccag cacgtcgagg
     1801 tcacggtccg ggccggcggc gacggccggg tgcaccaggg cctcgtggta gcggtacgag
     1861 tccacgctgg acagctggag gtcgccgtcg aggaacaggc gggtgtcgcg ccggccgtcc
     1921 cggtgccggc gctcggtgac cacgatgtcg gaccggcccc ggtcgtcgac cgccaggatc
     1981 gggtcgcggt acaggcgctg gcgtccgtcg tccacgaccg tcggggccag gaccaccatg
     2041 gcagcgagca ccagtgcgcc ggggacgacc agagccccct ggcgccacgg cacccccatg
     2101 accaccggga ccgcggcggc cagtaggagg ttgacggcgg cgatggccat cgtggcgccg
     2161 acgaggccca gcacggggcg gacggcgaac gcgaaggcga tggcgccgag gaacgcgccg
     2221 aggtagtcgg cggcggtgag cttggcgacg acggacgcgg cgcgctggcg ggcgagctgg
     2281 tcgttgagcg cggcgagcag cggcatctcg gcgccgatgc aggccccgat ggcgaaggcc
     2341 accaccagga gcgggcccca gaacgtgtcg agtgaggccc acagccagta cagcgccggc
     2401 gcggcggccg cgccgaggat cgccaggacc acctccacgc ccacgaaggc ccgcaccggc
     2461 cggccggcga ggcggccgcc gaacgccgcg ccggcaccca tgccggccat ggcggcaccg
     2521 agcacgacgg cgttggcccg ctcggtggcc ccgaccgtga cggtgccgag gaggagcagc
     2581 gacagctcgt aggcgacgcc ggcggcggcc agcaggccgg ccaccacgag cagggcgaag
     2641 cgccgggcgc gcaggtcggc cggctcgggg agccgggcgg cgctcacggg aggtcccgtc
     2701 gtccggtcag gacagcgcca ccgccgcggt gagggcgacg gccacctcgg ccgcaccggc
     2761 cacgagcacc cggggcgaga acccgggctc gttcacgacg ttgcggatgc cgccggggat
     2821 gagccagtcg aggcccagga acgcgagcac ggagaccgcc aggctccaga gggcctggga
     2881 ggcggcgtac acgatgccgt cgccgagctc gtcgggcgcc gtccagaccg acacgaagac
     2941 gatggcggcg acggcgagca tcttggccga caccaggatg gcggcgttga tgttgtcgcg
     3001 gatctgggtc cgcaggtgcc ccggcgtcag cacgtcgatg atgacgaacc cgacggcgaa
     3061 cagcacgaag gacgcgaccg tccacgccgc ggccgcaccg acgtcgtcga gcaccccgtc
     3121 ggtggtggca gcgagcagca tcgtcgtcat ctcatctctc cttcgagaaa ggggtggggg
     3181 tgggcgttcg ccggagggtc acttgccgaa gcctccgcca ccaccacgga agccaccgat
     3241 cccgcctcgg gaccgggcgc gggtgtcgaa gtcgtcgagg gtgccggacc agcccgggag
     3301 cagcaggatc gtcgggaagc gccgggtcgc ggcgcggttg tcctcgaaca ccaccacctc
     3361 cgaaccgtcg gccacggggg tgatggcgac cgtcgcctcc tcgtacagca agaagatcga
     3421 ttccccggac gagtcccgct ggctcggggc gcgcaccgcc tggatctcgt cgccgacggc
     3481 ggcgggcggg aaccccggtg ccgggaacgt cgtggtctcc cccgcgccgg tgtccggcgg
     3541 gcgctggtcc cggggtccgg tgaccggcag cgcgtcgatc gaggcgatga ggtcgtcggc
     3601 cgacaggtcg ccgctggccc cgatcgatcc gatgaaggag gagatcagca tgatgaagat
     3661 gacgatcgcg atgatcatcc cgatgccggt cgagccgcag ccggtgcacc cgaagccgtt
     3721 ggccatcggc ggtcgagccc gcggggtgta ctcccagcgc ttccaggcat cccaggcgcc
     3781 ctcgtcctcc ggttggtcac cggagccggc ggaccacccc cgccatgcgt cccacggatc
     3841 cggctggccg gagccgccgg gcacgtcgct caccgggccg ccacgtgggt ccgggtccgc
     3901 acggcgtggg gccgctcgcc cgggtacagg tgccaggtct cccagccgtc ggccaggacc
     3961 cggatcgagg tcagcgcatc gcggtgaccg gggaacgaca ccacgagcgt cgcatcgtcg
     4021 tcggcgcccg accggagctc ggcggcgagc acccccagcg ccgacccgtc gaggtcggga
     4081 tgctcggagg cgaaatcgag gcggtgcgcc ggggactcgt ggctggcgtg ctcgggaagg
     4141 ggctcgtccg gtgccgggtc gacgcaggcg acctgctcgg tggccacgac gccgtcgtcg
     4201 tcgagcacgt ggacccggtg ggaggcggcg aggacctcga ccaccaccgc gagcccgagc
     4261 gcctcgagcg tgccgaacag cgtcccgacg acgtcgaggc cgggcgtgcc gtggaagcgc
     4321 agggccaggc cgtccgccga cacgtcggcg gtgccaggtc gactgtcgag ctcgaggtgc
     4381 aagaccgctc cggggtcggg gctgcaggtc gggacacggc acgccggcac cgtggggggt
     4441 acgtaccata ggcgccgtgt tcgatccccg tgccgatctg tcccccggcc gacgcgtccg
     4501 catcgacggc aggcccgggt cggtccgggg cctgatcgac ctggaggagg agggcgaccg
     4561 ctggctggag gccctcgtgc agctcgacga gggtctcgcc gtgtggctct cggtcgagtg
     4621 gatgccgacc ccggtggcca ccgtctggct cccggtccgg cccaccgagg tcgacggcgg
     4681 accgggcgag aagcaggtgc gcctcggcgg caccagcttc tccttctacg agaacggcac
     4741 ggccagctac cgcgccaccg gcgagacggg cacggagccg gccggtcggg tcgactacgt
     4801 cgactacgtg gccgacgact cccggcgcgt ctccttcgag cgctacggcg aggccggcgc
     4861 cggacggcgc tccaccatgc tcaccggcac gtgcccgcag tgcggcgcgc cgtggcagcc
     4921 cacgtccacc ggccactgca cgtcctgcgg ggtgaacccc acggtcgacg tcggcgcctg
     4981 ggggacgtgg gaggtctcca tcggcatccc ggccaccaac gtcaccgccg cctgagccga
     5041 ccgccccacc tcagaggcag ccccgcagcg cctcgatcag gccccggctg cgggggtcgt
     5101 tcgagagccc ggagctcatc ttggacatga cgtagccgaa gccgaccccg tggtccgggt
     5161 cgccgaagcc gagcgagccg ccggcgcccg ggtgaccgaa gctgcgctcg ccgagcagcg
     5221 gcgagaagct cgacgtcagc atgaagccga ggccgaagcg ggtgggcatg atcagcaccc
     5281 ggtcgcggcc gccgctgtgc tccttggtcg cccgctgcag cgtgtcgtcg tcgagcagcc
     5341 ggaacccgtc gacctcgccg acgcaggcgg cgtacatctt cgccagcgag cgggcgttgg
     5401 tgaccccgtt ggccgccggg atctgcgacg cccgcacctc gggcaggttc cacgccaggc
     5461 gaccgccacc gaggaggaac gtgccgtcga gcatcagcgc ccggttggtc gtgctgttcg
     5521 gatcgaggaa cgccgtggcc atgtcctgca ggagcgggag gatctccgga tcgagcgatg
     5581 ccatgtcgaa gtcgtccgcg ctcgggcgtg acgggacgag cggcaccacc cggtcctgct
     5641 ggtcgtcggg caggccgatc cagaagtcca gcccgagcgg gccggcgacc tcctcggcga
     5701 agaactgccc gatgggccgg ccgtcgatgc ggcggacgac ctcgccgacc agccagccgt
     5761 aggtgagggc gtggtagccg tgcgcggtgc cgggctccca cagcggcgcc tgggcggcga
     5821 gggcctcgac gatcggggtg acctgcagga cctcctccag cgacggcggg tcgtcgacca
     5881 cggggacgcc ggcctggtgg tcgagcagca tcgcgatcgt ggtgccctcc ttgccggcct
     5941 ggccgtactc gggccagaca tcggtgacca gggcgtcggg gtccagctcg ccccgctggg
     6001 ccagcaggtt ggcgcagacc gcagcggcgc ccttcgtggt ggagaacacc agctgcaggg
     6061 catcggtgcc gtagggcgtc tcgtcggcat cgctcgtggg gtcggccgtg ccgccgacca
     6121 ggtcgacgac gagctcgccc tcgacgtaga cggcgacgcc ggcaccgagc tccccgtggg
     6181 tctcgaagtt ggcagcgaag gcgtctcgga cgggctcgaa gccgtccgcc acatgtccgg
     6241 agatcgtcat gccgggacgc tacacagtgg cccatggcac acgtggagga cccgcccggc
     6301 gtcgacctcg aggccctgcg ggcctggatg gacggcgagg ggctggggtc gggtccgctc
     6361 gaggacgtcg agcagctggc cggcggcacc cagaacgtgc tggtccgctt cacccgcagc
     6421 ggtcggggcc acgtcctgcg gcgaccaccg ctgcacaagc gagcgaactc cgacgagacg
     6481 atgcgccgcg aggcccgcgt cctggctgcc ctggccggct cc
//
LOCUS       DATISU010000133         1632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_285816, whole genome shotgun sequence.
ACCESSION   DATISU010000133 DATISU010000000
VERSION     DATISU010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285816"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VLR27_05430"
     CDS             complement(<1..414)
                     /locus_tag="VLR27_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HSP02920.1"
                     /translation="MAKTDANPPLPEFRDDYVLQEVDPSWLAAAVKPKQFIHIDQSEC
                     IMCEGCVDICPWKCIHMVSPSAIESATGTEQPGLDPSDHVIFTIDDDVCTRCALCVDR
                     CPTGVIILGKLGAPSAAGDPHARTPTHGYGYGMRLG"
     gene            complement(422..874)
                     /locus_tag="VLR27_05435"
     CDS             complement(422..874)
                     /locus_tag="VLR27_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02921.1"
                     /translation="MVAFLLSTIIGIIGILAVIWYGKNRRAPGAPLTWGQANAAAAFT
                     FFFMFWWYGVIPHQWLTWADNELNWRPDRTLWAVGNVLRPEAEGGWLPLTLNWRIVRD
                     LVAVVIYGVGLGLQIWLWAWWNDRKKKAEAQAAVVPTSSYGRPLVKKG"
     gene            1070..>1632
                     /locus_tag="VLR27_05440"
     CDS             1070..>1632
                     /locus_tag="VLR27_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02922.1"
                     /translation="MTEIPEHLLKRSRERRSAMGGDGGAAEGGGASDTSGAAASSTPA
                     KAAAPAPSSMPAHPAPAEVESAPPPSPMVEAYQKRRKIPFWALPVLAFLPLWAYVYTG
                     TLSPPPAGEGPEVLGEELYAGSGCGGCHGAGGGGGVGPGFTGGAIYETFPDFVTHFEW
                     VRLGSAGWQEERGGTYGANETPVGGGMP"
BASE COUNT          236 a          561 c          600 g          235 t
ORIGIN      
        1 accgaggcgc atcccatagc cgtagccatg ggtcggggtg cgtgcgtggg ggtcgccggc
       61 ggcgctcggt gccccgagct tgccgaggat gatgacgccg gtcgggcacc ggtcgacgca
      121 cagggcgcac cgggtgcaga catcgtcatc gatggtgaag atcacgtgat cgctggggtc
      181 gaggcccggc tgctcggtgc cggtggccga ctcgatggcc gacggcgaca ccatgtggat
      241 gcacttccac gggcagatgt cgacgcagcc ctcgcacatg atgcactcgg actggtcgat
      301 gtggatgaac tgcttgggct tcaccgcggc ggcgagccag gaggggtcga cctcctgcag
      361 gacgtagtcg tcccggaact ccgggagcgg cgggttggcg tcggtcttgg ccactagcgg
      421 atcacccctt cttcaccagc gggcggccgt agctcgacgt gggcacgacg gccgcctggg
      481 cctcggcctt cttcttgcgg tcgttccacc aggcccagag ccagatctgc aggccgagcc
      541 cgacgccgta gatgaccacg gcgacgaggt cgcgcacgat gcgccagttc agcgtgaggg
      601 gcagccagcc gccctctgcc tcggggcgca gcacgttgcc gacggcccag agggtgcggt
      661 cggggcgcca gttgagctcg ttgtcggccc aggtcagcca ctggtggggg atgacgccgt
      721 accaccagaa catgaagaag aaggtgaagg ccgcagcggc gttggcctgc ccccaggtga
      781 gcggggcccc gggcgcgcgc cggttcttcc cgtaccagat cacggcgaga atgccgatga
      841 tcccgatgat tgtggagaga aggaaggcga ccacgacgga cctcgtgaat tcgatcacat
      901 tggtgcgagg aaggctccgg agccgtcgga gcccgcaagt ggacggtcta ccacgacccc
      961 ctgaggcgag acacatcggg gcctttgggg cggcttgggt gcggatcgcg cccggcgcct
     1021 accctgtcgc cgttgcaaca agcgttcgcc gtgtcctccg ggaggcccgt tgactgagat
     1081 ccccgaacac ctcctcaagc gctcgcgaga gcgtcgctcc gccatgggcg gcgacggcgg
     1141 tgcggcggaa ggtggcggcg cctcggacac cagcggtgca gccgcgtcct cgacgccggc
     1201 caaggcggcc gcgccggccc cctcgtccat gcccgcccac ccggccccgg ccgaggtcga
     1261 gtcggcaccg cccccctcgc cgatggtcga ggcgtaccag aagcggcgca agatcccgtt
     1321 ctgggcgctg cccgtgctcg ccttcctgcc gctgtgggcc tacgtctaca ccggcaccct
     1381 gtccccgccg cccgccggcg aggggcccga ggtgctcggc gaggagctct acgccggcag
     1441 cggctgcggc gggtgccacg gcgccggcgg cggcggcggg gtcggtccgg gcttcaccgg
     1501 cggcgccatc tacgagacgt tccccgactt cgtcacccac ttcgagtggg tccgcctcgg
     1561 ctcggccggg tggcaggagg agcggggcgg cacctacggc gccaacgaga cgccggtcgg
     1621 cggcggcatg cc
//
LOCUS       DATISU010000134        32981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_658145, whole genome shotgun sequence.
ACCESSION   DATISU010000134 DATISU010000000
VERSION     DATISU010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 32981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32981
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658145"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /gene="lepA"
                     /locus_tag="VLR27_05445"
     CDS             <1..1488
                     /gene="lepA"
                     /locus_tag="VLR27_05445"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HSP02923.1"
                     /translation="DASQGIEAQTLANCYQALEHDLEIIAVLNKIDLPAAEPDRVAEE
                     IEHVLGLPAEEILHVSGKTGDGVTELLDAIVEKIPAPVGEADAPLRALIFDSHYDTYR
                     GVVSSVRIMDGTMRTGEKLRFMQARSTYDADEIGVRNPVPTPVDALGPGETGYLIAGI
                     KDVAEARSGETVTTASHGAEEALSGYQDPKPMVFAGLYPVDGDEYADLRDSLEKLRLN
                     DGSFTYEPETSMALGFGFRCGFLGMLHMEIIRERLSREFDLNLIITAPSVAYEVVTTT
                     GERIGVSNPSALPEPTEIDWIDEPMFSISILTPAEYLGPIIELCENRRGEQIRMEYLS
                     PERVEITYKIPLAEVVRDFFDQLKSRTQGYASLDYELVGYERANLVKVDVLLNSVAAD
                     AFSSIVHRDKAYDYGSKMAEKLKELIPRQMFDVPIQAAIGGKIIARETVKAKRKDVLA
                     KCYGGDISRKRKLLEKQKEGKKKAKSIGRVEVPQEAFVSALRLDD"
     gene            1485..2417
                     /gene="rarD"
                     /locus_tag="VLR27_05450"
     CDS             1485..2417
                     /gene="rarD"
                     /locus_tag="VLR27_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HSP02924.1"
                     /translation="MTEQRTGVLAGFSAYALWGVFPLVFRLLDEVSAVEILFHRIAWS
                     LLVVLAVLALQRRFTELATLVRHRRSLQRLALAAVFISINWLTYIWAVNSGHVVDAAL
                     GYYVNPLFTVALGVVVLHERLRPAQKVALGFGVASVLVLTVANGRVPWIALVLAGSFG
                     MYGYLKKDADAAALTSLAIETAVLAPLALVGLTVFAAQGELSFTNGSPWRDALLAGLG
                     VVTAVPLLLFATAATRIPLSMLGLLQYITPTMQLIVGVAILGESLPPARLAGFALVWI
                     ALTVLATDAVQSERRSRRVTVEPVTVEPITVEPI"
     gene            complement(2514..3803)
                     /locus_tag="VLR27_05455"
     CDS             complement(2514..3803)
                     /locus_tag="VLR27_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HSP02925.1"
                     /translation="MTDGTDYGFDTMLVHAGTEPDPATGAVQVPIYQSTSFAFRDAEH
                     AAKLFNLEEVGFIYSRLTNPTVWALQNRMAALEGGAGAVACSSGHAAQLMALFPLMAP
                     GRNVVVSTRLYGGSITQFSQTIKRFGWSATFVDFDDTDAVAAACDDDTRAIFCESIAN
                     PGGYITDLPAIAAIADAQGVPLIVDNTSATPYLCRPIEHGATLVVNSTTKYLTGNGTV
                     TGGVIVDSGKFDWMASDKFPSLSQPEPAYHGLVFAEALGPMAFTFHGIAVGLRDLGMT
                     MNPQGAHYTLLGIETLSLRMARHVENAEKVAGWLEGNDHVDAVTYAGLESSPYHHRMA
                     TICPKGAGGLFTIALKGGYDACVKFVDSLELFTHVANLGDTRSLVIHPASTTHRQLSP
                     EQLEAAGAPANVVRLSIGIEDPKDLIADLEQALAAAH"
     gene            complement(3945..4451)
                     /locus_tag="VLR27_05460"
     CDS             complement(3945..4451)
                     /locus_tag="VLR27_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02926.1"
                     /translation="MRRTLTWKAAVVGGAALGAGFGSIALAGADSSSTPPDSVELQEI
                     DRQISAISAPLSVLPAPAADIQSVSAATDPSSPAAPSAPSVQSAPSVQSAASMQSAAS
                     MQSAPSAPSPVQVAPAQQDPAPPPAPVADSPDSPASAPAPAPAPAPADDDSPDSPASA
                     GSVGSIDS"
     gene            complement(4451..5782)
                     /locus_tag="VLR27_05465"
     CDS             complement(4451..5782)
                     /locus_tag="VLR27_05465"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HSP02927.1"
                     /translation="MEIDDPAVPAVAHLTGAGAIDVLGAAVRTAGGTLHGARTSQVQY
                     RPGSDLVVRYRVDVTWADGRRRSNETLLASTSRTGALPGAIPVVADADDVQLVTSVWR
                     WPFDPVVVGLEDAVTPGRTDDLVGSITGDRPAVEVVAYRPTERAVVGLTGSDGTEAYL
                     KAVPPALLPDLVDRHERLLAADLPVPEVLATDAARGLVLLRALHGTTFRERVKADLPG
                     WPTPSAIADLVDRIHRADPGGLPTRPGRIADGIGHAQLVAAVVPALAPALDVLTTRFA
                     AEAGAVAARSGVAVHGDLHEGQLIIDRDGEVTGLLDLDDLAAGDPVDDLATLIGHLRF
                     RAGGVDPGSAIAGRLRDHVRALHDHATALVGAEAIDVAVAAVLVGLATGPYRVQHEGW
                     PDRVAAVVAEATSLVEPWTDMRKFSAGAHDGLTSHPHGGHRHPLPLMKGSA"
     gene            5888..6250
                     /locus_tag="VLR27_05470"
     CDS             5888..6250
                     /locus_tag="VLR27_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02928.1"
                     /translation="MLWRTLGALLLGAVVALAVGNAVIALGGDDDPQPPDAIRLAPDR
                     IGDPDVEPPVPTTRDADDPVVVVPPPVHLDPTTTPPPPPPAPTSPAPVAPPPPTDFDD
                     DDDFDDDDDVDDDDGDDD"
     gene            6250..7707
                     /locus_tag="VLR27_05475"
     CDS             6250..7707
                     /locus_tag="VLR27_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP02929.1"
                     /translation="MGGRVRQLLASTRARVLVGALVLLGGTIAITVVADRAVLLARLD
                     DRIDDELAQEVEEFARLADGIDPGTGEAFGSDLAAIFDTLFGRNIPAPSEVFLGIVDG
                     QPYLRSANAPYPIDELDALVTAWASESRPAYRTVSTPIGEMRTLVVPVVEPGGDLAGT
                     FVVGRFPGQERESVDEAIRVAALVGLIAFLGASAVAWVIAGRVLAPLRQLATGAASIR
                     EDQLDQRIPVEGTGELAALSRSFNDMLDRVEGAFAAQRSFLDDAGHELRTPITVIRGH
                     LELADPSVPLGSDTRALVFDELDRMARIVEDLLLIAKAERPDFVVPGPTDIADLVRDI
                     VQKARPLAERAWELRNDALVVAEVDRERVTQALMNLARNAMQHTSEGDRITVWSALSD
                     DRLELGVTDTGEGVAPDDRERIFERFARGSSAQRTRSDGAGLGLAIARAIAHAHDGHL
                     ELRDTPGGGATFVLSLPRPAEPDAPPTEEPMWPAS"
     gene            7692..8354
                     /locus_tag="VLR27_05480"
     CDS             7692..8354
                     /locus_tag="VLR27_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP02930.1"
                     /translation="MARILIVEDEDRIVSFLTKGLEANGHSTMAVGDGITAAAVADDG
                     DFDLMILDVGLPGKDGFAVLEEIRGRGVRMPVIVLTARDATSDTVTGFDAGADDYVTK
                     PFRFEELLARVRARLREQGTTEQTLLEAGTVVLDLRRRTVSVDGRSVELSSREFALAE
                     TLFRHPGQVLSREQLLSMVWGYDFEPESNIVDVYIGYLRRKLGAERIETVRGMGYRLR
                     AG"
     gene            complement(8357..9436)
                     /locus_tag="VLR27_05485"
     CDS             complement(8357..9436)
                     /locus_tag="VLR27_05485"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HSP02931.1"
                     /translation="MPQLSPRQWAIVGGVLAVIVAVIAVIALSGGDDEEPAERAVTTT
                     TTTTTTSTTAPPEPGYPLTGQPIDDPARAQRPVLAVKIDNAEPRTGRGGRPQAGINQA
                     DIVFEEMVEGSVTRFLALFHSQDADPVGPVRSARTTDLLLMEPLNRPLFAWSGANAGV
                     VGAVREAPVADVGLDVNSGAYYRAGDRPAPYNLMSSTGALYGGAASEQRQPPALFQYR
                     PADAEPTGDPATGVRIVFGGGPGSAPVDYEWDGTGWARTQRDSAHVDAAGARVAPPNV
                     VVQFTPYAEVQCCDASGFPIVEAQLVGEGDAWVFSAGRRIEARWSRPAVGDVTTYTGP
                     DGQPVLLTPGPTWVAVAPPGTAEAR"
     gene            9502..9702
                     /locus_tag="VLR27_05490"
     CDS             9502..9702
                     /locus_tag="VLR27_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02932.1"
                     /translation="MSDQSNEQQTGDLDELLGLDAASELLGVPVEQVRAMADQGVITR
                     HDRDGGPGFRRAELIAARDLGG"
     gene            complement(9709..10353)
                     /locus_tag="VLR27_05495"
     CDS             complement(9709..10353)
                     /locus_tag="VLR27_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HSP02933.1"
                     /translation="MRIDVWSDVVCPWCYLGKRRLEVALEGLDFADEVEVRWRAFQLD
                     PTATAEPKDLATAIDRKYGPGAFQGMTGRLVPLGQEVGIDYRFDLAQRVTSLPALSLV
                     AWVESEHGPDTAAQLHDRLFRAYFTEGANIADAANLVTWAVDVGADRELAGEAVATGT
                     GRDAVAADLEGAVDRQITGVPAFVIEDAFMIPGAQDVDTIRSLLTRMHERQTAS"
     gene            complement(10394..10789)
                     /locus_tag="VLR27_05500"
     CDS             complement(10394..10789)
                     /locus_tag="VLR27_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HSP02934.1"
                     /translation="MTAGLLVLGGCGDDDDSGGAASDASTSIAAEAADFEFDPDAWTV
                     PVDEEITIDFENAGNIEHEWAVLALGQEIESEADFSEEIVLFEVEAIPPGESTSESFT
                     VDEAGTYQVICALEGHFDAGMEGALEVAG"
     gene            10962..11930
                     /gene="bioB"
                     /locus_tag="VLR27_05505"
     CDS             10962..11930
                     /gene="bioB"
                     /locus_tag="VLR27_05505"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HSP02935.1"
                     /translation="MVETTPRHDWTTDDALALLERPFHDLLLDAQQVHRAGFRPDVVE
                     GAILLSIKTGACPEDCAYCPQSAKWNTDLEPERLMETDEIVAAATNAKASGASRFCMG
                     AAWRSPNDRQVERVAEAVAAVGELGMETCVTLGMLTDAQAQRLADAGLDYYNHNLDTS
                     PDFYGEIITTRTYQDRLDTIGHVREAGVRLCCGGIVGMGESRRQRAGLLAQLAQLDPH
                     PESVPINLLVQVPGTPLHGTDELDGLELVRTIAAARIMMPGSVVRLSAGRSQMSDELQ
                     ALCMHAGAASIFLGDKLLTTGNPGDDADADLLERLDTSLAEASLLD"
     gene            11951..12610
                     /gene="ung"
                     /locus_tag="VLR27_05510"
     CDS             11951..12610
                     /gene="ung"
                     /locus_tag="VLR27_05510"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HSP02936.1"
                     /translation="MRTDWNPLLRDQFEEPYWHELQAFVRDERARHEVYPPHDEVFAA
                     LHLTPYADVKVLILGQDPYHGPGQAHGLCFSVRDGIDIPPSLVNIHAELEADLGCPRP
                     GHGNLEHWARQGVLLLNTTLTVRARQAASHQRKGWERFTDRVVEVVDAKPERVVFVLW
                     GASARRKAAQIDTSKHTIIESPHPSPLSAHKGFFGTRPFSRANAALVEAGRDPIDWCI
                     P"
     gene            12598..14130
                     /locus_tag="VLR27_05515"
     CDS             12598..14130
                     /locus_tag="VLR27_05515"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF002868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HSP02937.1"
                     /translation="MHPVTLLADVVAAADAVGATTKRTAKREVLAELFAGLAPDEVPV
                     VVGFLLGEVRQGRIGVGWAAIRDLDCPAGDAASLSVDEVDRAVSVLASTTGPGSQAAR
                     RSQLVALFSRATAPEADFLGRLFVGEMRTGALAGVITDAVAAAAGIPAATVRRAVMLA
                     GDLGAVARIALTEGREAVEAVGLEPLRAVQPMLASTSESVAAAIDEVGEASVEWKLDG
                     ARIQVHRSGDEVRIYTRNLNDVTGRLPEVVEVVRSFDSTSFVLDGEVLGFMGPDQAPQ
                     AFQDTMSRFGRGAGVGALVPQFFDVLHLDDADQIDQPLRVRRERLAALVGPHLIPGTT
                     TDDPDVGEGVLEDALAHGHEGVMVKAADSSYEAGRRGKSWRKVKPVHTLDLVVLAVEW
                     GSGRRKGWLSNIHLGALDPATGEPVMVGKTFKGMTDEMLAWQTARFQELETHRSGHTV
                     HVRPEQVVEIALDGAQTSSRYPGGVALRFARVVRYRDDKTAAEADTLNAVRSLLAGPA
                     IP"
     gene            14127..15473
                     /locus_tag="VLR27_05520"
     CDS             14127..15473
                     /locus_tag="VLR27_05520"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HSP02938.1"
                     /translation="MTTSLLWFRRDLRLSDNPALVEAIRSADDVVGVFCLDPALLGPA
                     GDVRRHHLFRTLAALDQSMDGNLVVRTGDPIAVIPELAVEVEAASVHLAEDFGPYGRR
                     RDDAVAERVTGAGMELVRTGSAYAVPPGSIQNKSGTPYKVFTPFSRAWREHGWPDPVR
                     RPSAIPWRSAPSDGIPDLPALGDVELPRAGEEAAGNRLDHFLRAKVEDYDEARDLPGV
                     DATSRLSPDLKWGTLHPRQILARLGPSKGAQTFATELCWREFYADVLLHRPESARESM
                     DDKVGAMPWDRGAGADDLFEAWTRGCTGFPIVDAGMRQLLAEGWMHNRVRMITASFLV
                     KDLHLDWQRGARWFMQHLVDGDLASNQHGWQWTAGTGTDAAPFFRIFNPTTQGQRFDP
                     DGTYVRRWIPELSGVPSKWVHTPADAPDGLPAGYPEPIVDHREEREESLRRYGATR"
     gene            15488..16924
                     /locus_tag="VLR27_05525"
     CDS             15488..16924
                     /locus_tag="VLR27_05525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HSP02939.1"
                     /translation="MAMIAPQDLLFLLMEAREKPAHVGGLALFETPDDADEQYLYELY
                     QSSIASDAPVRSLFQRRVSRPGLAGYSWTRDDQFDIQYHLRHSALPVPGRIRELLTVV
                     SRLHGSLLDRNRPLWEAHLIEGLADGRFALYTKIHHALCDGVTAMKLLESSMSPDPTE
                     RGMPQPWERRPRGSMFDEPDPDEVAPPSRPAAQDDTWTAVLSDGLRKLGSSVTESLGG
                     VVGATDGIGHLLSSFRDQSLSSPLRAPRSMLNVPITGARRFAADSWSIDRIRAAAAPH
                     GATVNDMVLAMSAGALRTYLLDQGALPDQPLVAMVPVSLKTRGADERSGNAVGAILCS
                     LGTHLDSAPARLAHIQESVNQSKARLEGLSPGQILALSALNVAPLALNMSPLGRMPLA
                     EERIRPPFNVVISNVPGPKETLYWNGARLSGSYPMSLLVDGQALNITTSSYAGNLDFG
                     ILGCRRTVPSMQRLLRYLDDELVALERG"
     gene            17005..18012
                     /gene="hrcA"
                     /locus_tag="VLR27_05530"
     CDS             17005..18012
                     /gene="hrcA"
                     /locus_tag="VLR27_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HSP02940.1"
                     /translation="MLDDRKASILRAVVQEYIETAQPVGSGRVSAAPGVDVSSATVRN
                     EMAVLENEGYLAQPHTSAGRIPTEKGYRYFVDTIGAPGALRGEQAKQVKAFFARAHGE
                     LEEMFESTSRLLSGLTAWTGVVVPPSAADVAIRSVQVVGLAPSTALVVTVLADAMVEK
                     HSVELPGDVGDDVVARASLHLASELTGTMRGAVPPDVAPTGDPDTDGVVGAVVEVLRR
                     TTPADTADEVFVGGASRVASAFDAVETVQQVLSVLEEQLVVVTLLRDVVDRGLQVAIG
                     TETGMETLADAALVVAPYTVDGERAGSIGVLGPARMNYPEAMAAVAAVSRRLGHHLTE
                     G"
     gene            18027..19154
                     /gene="dnaJ"
                     /locus_tag="VLR27_05535"
     CDS             18027..19154
                     /gene="dnaJ"
                     /locus_tag="VLR27_05535"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HSP02941.1"
                     /translation="MADLYDLLGVSRDASQDEIKRAYRAKARELHPDANPDDPDAEHH
                     FKEVAQAWEVLRDPEKRARYDRFGDAGIGGAGQPGAGDPFAGFGGVGDIFDAFFGGQS
                     PFGGAGGGRSGPMRGPDLEVVTELDFTEAVFGAQHKVDVRTAVACEVCDATGATPGTS
                     ATTCAECGGAGQVRRVRQSLLGQMVTTAACPRCNGAGETIADPCGNCAGEGRTLQDRS
                     FTVDIPPGVDAASTLRLQGRGAAGPRGGPPGDLYVHFRVRPHDVFRRDGVDLHADLHI
                     PVTQAVLGAEVDFETLDGTETITIPPVTPTGHVFRFRGKGVPVVNRGGRGDLHLHVVI
                     DLPRDLTEEQEELVRRLAELRGEPVAEESRGFFTKLRTAFK"
     gene            19208..19840
                     /locus_tag="VLR27_05540"
     CDS             19208..19840
                     /locus_tag="VLR27_05540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HSP02942.1"
                     /translation="MPVLDEAALHHLSKVRRLRDGDALTVTDGRGAWRAGRFRGRAEV
                     ESDGPVVEVPAPNPEVTVAFALTKSDKPDLVVQKLTELGVDRIVPFRADRSVVRWDEA
                     KAARQHERLVAIARSACEQSHRCWLPTVEPVTDVADLAARGAVRLDRGPVAVSLDRPV
                     VAVGPEGGWSDSERAALPDTAGLGPHVLRAETAAITAGALLGAMRSGWGG"
     gene            19959..20489
                     /locus_tag="VLR27_05545"
     CDS             19959..20489
                     /locus_tag="VLR27_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HSP02943.1"
                     /translation="MAMEDLDEAGLAYSRKVGERLRAIRRQKRLSLHDVESASDSEFK
                     ASVLGAYERGERSISVPRLHRLAGFYDVPVDQLLPTDEEGEVIDLTDRALARQAENHL
                     VLDLQMLSELGGPEGAMLNRYIRTIQIQRQDFNGRMLTIRKDDVRAIACILGVATDRT
                     MDRLDQLGLCRVGASR"
     gene            20525..21637
                     /gene="hemW"
                     /locus_tag="VLR27_05550"
     CDS             20525..21637
                     /gene="hemW"
                     /locus_tag="VLR27_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HSP02944.1"
                     /translation="MPFCSTRCDYCAFATWTDRGHLVDRYLAGLATEVDRAVADGLPA
                     LTSVFVGGGTPTLVPADGLVDVLSRLPLAPGAEVTVEANPDSVTAESLRTYRRGGVTR
                     MSFGVQSMREHVLAALGRTHDPDNVRQAVAWTREAGFETFNLDLIMGAAGESVEDWAS
                     SIEDVLELDPPHVSAYGLTVEPGTPLAADPDRYPDDDDQADKYLLTDERLGAAGLRWY
                     EVSNWARPGHECRHNRLYWEAGEYLGFGCAAHSHRAGRRWWNLRTPERYLDAVEAGTS
                     TEAAGEDLDEGTRRLETLQLALRTREGVPVAALDLDDPSLEGLVDVVGDRVVLTPEGR
                     LLANEVAVRLRTDVAVGVGPRAQPGGAEGTGGTSKT"
     gene            complement(21592..22338)
                     /locus_tag="VLR27_05555"
     CDS             complement(21592..22338)
                     /locus_tag="VLR27_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02945.1"
                     /translation="MTRRAARSLLWLAGLLAAGVSLRLASRGELAAPPLGSFDQLTTW
                     VDGRGPAAAAVALVRLLAEVWVWYVLALSALHVASGALRAVGGQRLADALAVPAARRL
                     VHAGLGIGLAAASTVGGGDEGVAPGVATMTPVAEAPLVRAGGVEAAGGTATMRPLLSP
                     SGDHGTAVMTPTPRPSTWTVAPGESFWSIAEELLVDAWRRPPSDAEVDPFWRVLVEVN
                     RGRLTDPADPDAILPGQVFEVPPVPSAPPG"
     gene            complement(22335..23903)
                     /locus_tag="VLR27_05560"
     CDS             complement(22335..23903)
                     /locus_tag="VLR27_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSP02946.1"
                     /translation="MVIAVRTEEADGGLVVVKEARSDGDVARLTREAAMLARCRHPGV
                     VELLAVEERAIRLRHAGTALARLGPFPLDHAAGIVCAVADVVHALHRLGVVHGDLEAA
                     HVLLDDRGRPRLCGFGMAADRTDDGAADDVAAIGRLLGSLLRTGADLPWANPPSGVRR
                     AARQRQARKDLDAIVVQATRERRDQRLTARQLAKAVHAAVPECSLPTPSAARPAAPWF
                     AELPADVDPTADLGWTDEDLSWLAAVADDDRNADELEADAGPYAGLGSLDQLDDGTDE
                     DWAALAAAPEAVTGPLPVFTPPDEGPRERARIQHEATPPPAPGPSVPLPDRREHEEQR
                     RRLPLVRAALLLLVLAIGVVGGVAGARAVRPLGSDPVSATDATTTTTTAPGQTTSSTS
                     TTAAPEAGPWPERCTLPEPVGPDVDGDGCPDEVVLDGRTAQIGTVAVELGQDGDLVAL
                     GDWRCDGIATPALLRPDTGEVFVFPEWRLDEPVEVEALTTVPGATAIAASSGECPAPV
                     LSTPDGSRTVEVPE"
     gene            complement(23938..24381)
                     /locus_tag="VLR27_05565"
     CDS             complement(23938..24381)
                     /locus_tag="VLR27_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02947.1"
                     /translation="MSTARARDTRGSVLMLMPVAVLIVLLLGAIAVDSAIVYLGQRQA
                     HNVAFDAANDAAGAGFDLDAARTSGEIVYDPDRVHAIAAQAVAAAGIDDLELVSAVPD
                     GDSVVVTVRRTVEHLFVQALGDPARDVLTISARVGGEVHRPATEP"
     gene            complement(24378..24851)
                     /locus_tag="VLR27_05570"
     CDS             complement(24378..24851)
                     /locus_tag="VLR27_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02948.1"
                     /translation="MAAARTIRGERGQAGGMEVLPFALLIFVGGALLIINIWGVVDAK
                     YAADAAARESARWVVEAAGQTTTADALRAGATTIATDTLDDHGRTGHATVEVGPPGAA
                     FVRCERIEVTVSIEVPAIRLPFVGGFGDAFDVQATHGELVDPTRSGVDGAASCIQ"
     gene            complement(24875..25234)
                     /locus_tag="VLR27_05575"
     CDS             complement(24875..25234)
                     /locus_tag="VLR27_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02949.1"
                     /translation="MVFLLFTVQVLFALHARTTATAVASDLAQRAANEGAGLDRARMA
                     AYAAQGRQRLGGYGDDADFTFALTDADGDGRDDTVAVTVHAALPILLPVRWSGYSPTE
                     FSRTMRARLEVFQAAGP"
     gene            complement(25352..25546)
                     /locus_tag="VLR27_05580"
     CDS             complement(25352..25546)
                     /locus_tag="VLR27_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02950.1"
                     /translation="MSTTARTAHRRGDRGEGVISTAIAVLIMALLGAAMWLTFDNIWS
                     DTAQNTEDQVNVIGSDGSSG"
     gene            complement(25571..26482)
                     /locus_tag="VLR27_05585"
     CDS             complement(25571..26482)
                     /locus_tag="VLR27_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSP02951.1"
                     /translation="MTRVTIVATLAFWAGATLVLSRHRWFARRSLADRLRPYEPGGLA
                     TSAGGPGMLSVDSFADVIAPLARTVGERVARLVGINEALAVRLERVHSELDPTDFRVR
                     QLGWTAVGAVGGALAALAIGPPAAVVLLFLVGAPALSFLFVEQQLAMASDRRKRRLLL
                     ELPVVSEQLGMLLSAGFSLGAALQRLSTRGQGASGQDLRRVVGRVRHGVTEVEALREW
                     AAVADVDALTRLVAVLALNREAGDLGRLIAEEARSIRREVQRGLVEQIERRSQQVWIP
                     VTVAALVPGAIFIAIPFLEALRVFSAS"
     gene            complement(26479..27363)
                     /locus_tag="VLR27_05590"
     CDS             complement(26479..27363)
                     /locus_tag="VLR27_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02952.1"
                     /translation="MTALLLPVAAALGVYYLYTAVAFGWRGVGVAPAVGNRRDRRPVV
                     QEWLVQAGLGDVRLRDFAAVMVLLLVLGAALTFAIFGAVAPAIAVGLAAATAPLASYR
                     VRRANRRARAQDAWPRMIEEIRILTSSLGRSIPQALFEVGRRAPDDMVPAFDAAHREW
                     LISTDFERTLTVLKGGLADPTADATCETLLVAHTVGGSDLDRRLEALIEDRIQDTQGR
                     KDARAKQAGARFARRFVVFAPFGMALAGMSVGNGRQAFQSATGQVLTSVAVLLVAGCW
                     LWAGRILQVPEEERVFDR"
     gene            complement(27360..28673)
                     /locus_tag="VLR27_05595"
     CDS             complement(27360..28673)
                     /locus_tag="VLR27_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HSP02953.1"
                     /translation="MITVHTDATPLELIEGTVLEQAKGIDLDLDDDAARARMRDLITD
                     AVARWSADYRRGLRTHDLADPELLVERAYRNLVGYGPLESLLADDDVWEVMINAPDAV
                     FVKRHRGPSGYHDEVFHDDEHVIRTLTKILDDASGAHRKLDPTEGLQDAQLDDGARLH
                     IVHSDVASGGHVMVNIRKFTGVAFRELEQLVERDMLSAQAAEFLRACARAKLTVVFAG
                     APGSGKTTLLSCTAAELDPALRVVVAEEVFEADVPLPNVASMQTRAARPDRPAVDLRR
                     LVAGFLRMAPDVAIVGEVRDREALPLLLTLSSGVKGYTTIHAGSARQALSRLRFICQL
                     ADTSSEIPMSALNSLVSESIDIVVHSAREAGGPRVTEIIAVEDQAAGHDATQFTVTDL
                     FRRARRDQPLEWTGLLPNRAARALEEAGFDLRGLLGVPDRTRQDR"
     gene            complement(28670..29974)
                     /locus_tag="VLR27_05600"
     CDS             complement(28670..29974)
                     /locus_tag="VLR27_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02954.1"
                     /translation="MSERFVTLGLARIRAPWFSDVARWATSGQLAMDFVKTVSAAEVR
                     ARLESGRPFSALLVDGGMPGIDRDLLELAREHGCAPIVVVDGAARNWSTIGAVHVLPS
                     DFGRTELEAVLSEHARPIGRADDLPGQVQIPDRRDIGFRARVVAITGPGGTGRSLVAM
                     AVAQGAAADPTHADTVVLADLALHADQAMLHDARDVIPGVLELVEAHRSGVPSLEDVR
                     ALTFDIVARRYRLLLGLRRHRDWTALRPRALRSALDGLRRSFTLVVADVDDDLEGEAD
                     TGSADVEDRNLLARTTIGLAEVVVVVGNPGTKGAHSLLRVVRDCIANGVDADRIVPVV
                     NRAPRSRHARAEIHRTISELLAASNPGQPVLSPVFLPERKQLDDALRDGVALPEAFVA
                     PVSQAVQARLDAVDERTHLPGFDEPVPVQAGSLGSWDEEVAG"
     gene            complement(29974..30720)
                     /locus_tag="VLR27_05605"
     CDS             complement(29974..30720)
                     /locus_tag="VLR27_05605"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HSP02955.1"
                     /translation="MTSTLTRRNGRATAGPDPTPRRVERRLGLPSTRAVVGGLLMALA
                     AVGTFVAYADATADDTIEVLVASRDLAPGQTVRAGDVALVSVELPAGVRGLFGAVDAA
                     VGRQLVASVDAGEFLLASATAVPVEGDEHLEVALSLPTSRAVGGLRAGERVDVFSTWS
                     SEVTQLIAVDARVLEVGGGAREGLGGSDTTVVRLALSDFDQVEALVHAQAAGDITMVR
                     ATLGTEVEDVGREYRPRSVAGRSTHPDEDA"
     gene            30962..31213
                     /locus_tag="VLR27_05610"
     CDS             30962..31213
                     /locus_tag="VLR27_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP02956.1"
                     /translation="MTASVEWLSTADAAKALGITPRTLYRFIDQGELAAYRFGRVIRI
                     KQSDVDAFIEGARIEPGSLEHLYPDSGGKPEGAALDSEV"
     gene            complement(31210..31815)
                     /locus_tag="VLR27_05615"
     CDS             complement(31210..31815)
                     /locus_tag="VLR27_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02957.1"
                     /translation="MHHDDGSDLPAAARAAAPGVLGGTDALARSLERWAADALVDEAA
                     RQRTRQRWLRIQAEEEASTLGALVDLAEGGRPVVLDVGDHRIRGTIVGIGADFLAVRS
                     ERDQDVLVRTEAVEVVRAEPGGRPVTGDRTVLVEVALGAVVGPLAADRPQVLIRTRSG
                     QGVRGELRSAGTDVVRLRTDGDPPAPVWIPLGAIDVVVLEP"
     gene            31858..>32981
                     /locus_tag="VLR27_05620"
     CDS             31858..>32981
                     /locus_tag="VLR27_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HSP02958.1"
                     /translation="MSDTQVVVNVPGNHLMAGLLGQRDELLRLVERAFPDTAIHARGN
                     EIAVVGPEAPAAGKVFEELVLLVASGQLLDTESVQRAIDMVIHDERPSELMATEILRS
                     AKGNAVRPKTAGQRRYIEAIRDHVITFGLGPAGTGKSWLAVAMAVQALHRNEVERIIL
                     TRPAVEAGERLGFLPGDLMAKIDPYLRPLYDALYDMVDPEGAQRLLERNLVEVAPLAF
                     MRGRAQPVDRKVLTPTGWQEIGTLEVGDLVIGSDGMPTPVLGVFPQGRKEVFRVATQD
                     GSSTLACGEHLWTVKTLDDKKHGKPGRTLQTSDMVGNLRRHHQRFYELPMLSAPARFE
                     PQDVPMDPYALGLLLGDGCITGKTTPTFTTADPELAVALE"
BASE COUNT         4578 a        11829 c        12083 g         4491 t
ORIGIN      
        1 gacgcgtccc agggcatcga ggcccagacg ctggccaact gctaccaggc actcgagcac
       61 gatctcgaga tcatcgccgt gctcaacaag atcgacctgc cggcagccga gcccgatcgg
      121 gtcgccgagg agatcgagca cgtgctcggc ctgcccgccg aggagatcct gcacgtctcc
      181 ggcaagaccg gcgacggcgt gaccgagctg ctcgacgcca tcgtggagaa gatcccggca
      241 ccggtcggtg aggccgacgc cccgctgcgg gcgctgatct tcgactcgca ctacgacacc
      301 taccggggcg tggtctcgtc ggtccggatc atggacggca ccatgcgcac cggcgagaag
      361 ctccggttca tgcaggccag gtccacctac gacgccgacg agatcggtgt gcgcaacccg
      421 gttcccacgc cggtcgacgc gctcgggccg ggcgagaccg gctacctcat cgcgggcatc
      481 aaggacgtgg ccgaggcccg ctcgggcgag accgtcacga ctgcctccca cggtgcggag
      541 gaggccctct cgggctacca ggatcccaag ccgatggtgt tcgccggcct gtacccggtc
      601 gacggcgacg agtacgccga cctgcgcgac agcctcgaga agctgcggct caacgacggc
      661 tccttcacct acgagcccga gacgtcgatg gccctcggct tcggcttccg ctgcgggttc
      721 ctcgggatgc tccacatgga gatcatccgc gagcgcctct cccgcgagtt cgacctgaac
      781 ctgatcatca ccgcgccctc ggtggcctac gaggtcgtca ccaccacggg cgagcgcatc
      841 ggggtctcga acccctctgc cctgcccgag ccgacggaga tcgactggat cgatgagccg
      901 atgttctcga tctcgatcct cacgccggcg gagtacctcg gcccgatcat cgagctgtgc
      961 gagaaccgcc ggggcgagca gatccgcatg gagtacctgt cacccgagcg cgtggagatc
     1021 acctacaaga tcccgctggc cgaggtggtg cgcgacttct tcgaccagct gaagtcgcgc
     1081 acgcagggct acgccagcct cgactacgag ctcgtcggct acgagcgggc caacctcgtg
     1141 aaggtcgacg tgctcctcaa cagcgtcgcc gccgacgcgt tctcctcgat cgtgcatcgc
     1201 gacaaggcct acgactacgg gtcgaagatg gccgagaagc tcaaggagct catcccgcgc
     1261 cagatgttcg acgtgccgat ccaggcggcc atcggcggca agatcatcgc ccgcgaaacg
     1321 gtgaaggcca agcgcaagga cgtgctggcc aagtgctacg gcggcgacat ctcccgaaag
     1381 cgcaagctgc tcgagaagca gaaggaaggc aagaagaagg ccaagagcat cggccgggtc
     1441 gaggtgcccc aggaggcctt cgtgtccgcc ctgcggctcg acgattgacc gagcagcgca
     1501 ccggcgtcct cgccgggttc agcgcctacg ccctgtgggg cgtcttcccc ctcgtcttcc
     1561 gactgctcga cgaggtctcg gccgtcgaga tcctgttcca ccgcatcgcc tggtcgctgc
     1621 tcgtcgtgct ggcggtcctg gccctgcagc ggcgcttcac cgagctggcg acgctggtcc
     1681 ggcaccgccg ctcgctgcag cgcctcgccc tggccgcggt gttcatctcg atcaactggc
     1741 tcacctacat ctgggccgtg aacagcggtc acgtggtcga cgccgcgctc gggtactacg
     1801 tgaacccgct gttcacggtg gccctcggcg tcgtcgtgct gcacgagcgg ctgcgtccgg
     1861 cgcagaaggt ggcgttgggc ttcggcgtcg cgtccgtgct cgtgctcacc gtggccaacg
     1921 ggcgcgtccc gtggatcgcc ttggtgctgg ccggatcgtt cgggatgtac ggctacctga
     1981 agaaggacgc cgacgcggcg gcactgacgt cgctcgccat cgagaccgcc gtgctcgcac
     2041 cgctggcgct cgtgggcctc accgtgttcg cggcccaggg cgagctctcg ttcacgaacg
     2101 ggtcgccgtg gcgcgacgct ctgctggccg gcctcggcgt ggtcacggcc gtgccgctcc
     2161 tgctcttcgc gacggcggcg acccgcatcc ccctgtcgat gctcggtctg ctgcagtaca
     2221 tcacgccgac catgcagctc atcgtcggcg tggcgatcct cggcgagtcg ctgccgccgg
     2281 cccggctcgc cggcttcgcc ctggtctgga tcgccctcac ggtgctggcc accgacgctg
     2341 tccagtccga gcgtcgatcc cgacgcgtca cggtcgagcc ggtcacggtc gagcccatca
     2401 cggtcgagcc catctgaccc ctctcgaaac tggacgcgat tccggtgcac ggaagcccgt
     2461 tgtgccccgg gatcgcgtcc agttttcagt ccagttgcca gctgccgagt gctctagtgg
     2521 gcggcggcga gggcctgctc gaggtcggcg atgaggtcct tggggtcctc gatgccgatc
     2581 gagaggcgca cgacgttcgc cggggcgccg gccgcctcca gctgctcggg agagagctgg
     2641 cggtgcgtcg tggaggccgg gtggatgacg agggagcggg tgtcgcccag gttcgccacg
     2701 tgggtgaaca gctcgaggga gtcgacgaac ttcacgcagg cgtcgtagcc gcccttcagc
     2761 gcgatcgtga acagcccgcc ggcgcccttg gggcagatgg tggccatgcg gtggtggtac
     2821 ggcgaggact cgaggccggc gtaggtgacg gcatcgacgt ggtcgttgcc ctcgagccag
     2881 ccggcgacct tctcggcgtt ctcgacgtgg cgggccatgc gcaggctcag cgtctcgatg
     2941 ccgagcagcg tgtagtgggc gccctggggg ttcatcgtca tgccgaggtc gcgcaggccg
     3001 acggcgatcc cgtggaacgt gaaggccatg gggccgaggg cctcggcgaa caccaggccg
     3061 tggtacgccg gctcgggttg ggacagcgac gggaacttgt ccgaggccat ccagtcgaac
     3121 ttcccggagt cgacgatgac gccgccggtg acggtgccgt tgccggtgag gtacttggtg
     3181 gtcgagttca ccacgagggt ggcgccgtgc tcgatcgggc ggcacaggta cggggtggcc
     3241 gaggtgttgt cgacgatgag gggcacgccc tgggcatcgg cgatggcggc gatcgccggc
     3301 aggtcggtga tgtagccgcc agggttggcg atcgactcgc agaagatggc gcgggtgtca
     3361 tcgtcgcagg ccgccgcgac ggcgtcggtg tcgtcgaagt cgacgaaggt ggccgaccac
     3421 ccgaagcgct tgatggtctg ggagaactgc gtgatcgagc cgccgtagag gcgggtggac
     3481 accaccacgt tgcggcccgg cgccatgagg gggaacagcg ccatgagctg ggcggcgtgg
     3541 cccgacgagc acgccacggc accagcaccg ccctcgaggg cggccatgcg gttctggagc
     3601 gcccagaccg tcgggttcgt gagccgggag tagatgaagc cgacctcttc gaggttgaag
     3661 agcttggcgg cgtgctcggc gtcgcggaag gcgaaggagg tgctctggta gatcggcacc
     3721 tgcacggcgc cggtggccgg gtcgggctcg gtgccggcgt ggaccagcat ggtgtcgaag
     3781 ccgtagtcgg ttccgtcggt catcggaggg ttcctcttcg tcgcaagtgg cggggtggtc
     3841 gcgccgaccg taggtgatcg ccgggtcgtt ccgtcacccc ttcgtggacg cgacggagcc
     3901 cggacggggg cgggaccgtc cgggctctcg tcgggatgca gcggtcagct gtcgatggac
     3961 ccgacgctgc cggccgacgc cgggctgtcc ggcgaatcgt cgtcggccgg tgccggcgcg
     4021 ggggccgggg cgggtgccga tgcgggggag tccggcgagt cggccacggg tgcgggcggc
     4081 ggtgccggat cctgctgcgc cggtgcgacc tgcaccgggg agggggccga gggcgcggac
     4141 tgcatcgagg cagcggactg catcgaggca gcggactgca cggagggagc cgactgcacg
     4201 gacggcgcgg acggtgcggc cgggctggag gggtccgtcg ccgcgctcac cgactggatg
     4261 tcggcggccg gtgccggcag gaccgacaac ggggcgctga tggcgctgat ctggcggtcg
     4321 atctcctgca gctcgacgga atcgggcggc gtgctggagc tgtcggcccc ggcgagggcg
     4381 atggagccga agccggcgcc gagcgctgcg ccgccgacca cggcggcctt ccaggtgagg
     4441 gtgcgtcgca tcatgcactt cctttcatca gggggagggg gtgacggtgc ccaccatggg
     4501 ggtgggaggt gaggccgtcg tgagcgccgg ctgagaactt cctcatgtcg gtccagggct
     4561 cgacgagcga ggtcgcctcg gcgacgacgg ccgcgacgcg atcgggccag ccctcgtgct
     4621 gcacccggta cgggccggtg gccaggccga ccagcacggc cgcgacggcc acgtcgatgg
     4681 cctcggcgcc gaccagcgcc gtggcgtggt cgtgcagggc acggacgtgg tcgcgcagcc
     4741 ggccggcgat cgcgctgccc gggtcgacgc ccccggcccg gaaccgcagg tgaccgatga
     4801 gggtggcgag gtcgtcgacc ggatcgcccg ctgcgaggtc gtcgaggtcg agcaggccgg
     4861 tgacctcccc gtcacggtcg atgatgagct ggccctcgtg caggtcgccg tgcaccgcga
     4921 cgccgctgcg ggccgccacg gctccggctt cggcagcgaa gcgggtggtg agcacgtcga
     4981 gggcgggggc cagggccggc accacggcgg ccacgagctg ggcgtgaccg atgccgtcgg
     5041 cgatgcggcc gggccgcgtg gggagcccgc cggggtcggc tcggtggatg cggtcgacga
     5101 gatcggcgat cgcgctcggc gtcggccacc cgggcaggtc ggccttgacg cgctcgcgga
     5161 acgtcgtccc gtggagcgca cggaggagca cgagaccccg ggccgcatcc gtggccagca
     5221 cctcggggac ggggaggtcc gcagcgagga ggcgctcgtg gcggtcgacc aggtcgggca
     5281 gcagtgcggg cgggaccgcc ttgaggtacg cctcggtgcc gtcgctcccg gtgaggccca
     5341 ccacggcgcg ctccgtgggt cggtaggcga cgacctccac ggcgggacgg tcgcccgtga
     5401 tcgatccgac gaggtcgtcg gtgcggcccg gcgtgacggc atcctcgagc ccgacgacga
     5461 cggggtcgaa cggccagcgc cacaccgacg tcaccagctg gacgtcgtcg gcatcggcca
     5521 cgacggggat ggcgcccggc agggccccgg tccggctcgt ggacgcgagc agcgtctcgt
     5581 tcgatcgccg tcgcccgtcg gcccaggtga cgtcgacccg gtagcggacc accaggtccg
     5641 acccgggccg gtactggacc tgggacgtgc gggccccgtg cagcgtgccc ccggcggtcc
     5701 gcacggcggc gccgaggacg tcgatcgcgc ccgcaccggt gaggtgggcc acggcaggca
     5761 cggcggggtc gtcgatctcc acgcgatcca tcctcccggc gaggggtgag gacggcgtga
     5821 gacgaccgtg agaacgttct catcatcgcc tcacgcacac ctcacgcggg ggagggcacc
     5881 atggggcgtg ctctggagga ccctcggtgc cctgctgctc ggcgccgtcg tcgccctcgc
     5941 cgtcggcaac gccgtcatcg ccctcggggg cgacgacgac ccccaacccc ccgatgcgat
     6001 ccggctcgcc cccgaccgca tcggcgaccc cgacgtcgag ccaccggtcc cgaccacccg
     6061 cgacgccgac gatccggtcg tggtcgtccc gccgcccgtg cacctggacc cgacgacgac
     6121 gccgccgccg cctccgccgg cacccacgtc accggccccc gttgccccac cgcctcccac
     6181 cgacttcgac gacgacgacg acttcgacga tgacgacgac gtggatgatg acgacgggga
     6241 cgacgactga tgggcggccg ggtccgccag ctcctcgcca gcacccgtgc ccgcgtcctg
     6301 gtcggcgctc tcgtcctgct cggcgggacg atcgccatca ccgtcgtggc ggaccgggcc
     6361 gtcctgctcg cccgcctcga cgatcgcatc gacgacgagc tcgcccagga ggtcgaggag
     6421 ttcgcccgcc tcgccgacgg gatcgacccg ggcaccgggg aggccttcgg ctccgatctg
     6481 gcggcgatct tcgacaccct cttcggtcgc aacatcccgg cgcccagcga ggtgttcctg
     6541 gggatcgtcg acgggcagcc ctacctgcgc agcgccaacg ccccctatcc gatcgacgag
     6601 ctcgacgcgc tggtcacggc atgggcgtcg gagagccgac cggcctaccg caccgtgtcg
     6661 acaccgatcg gcgagatgcg caccctcgtc gtgccggtgg tggagcccgg tggggacctg
     6721 gccggcacct tcgtggtcgg ccggttcccg ggccaggagc gcgagtccgt cgacgaggcg
     6781 atccgggtcg cggccctcgt cggcctcatc gccttcctcg gcgcgtcggc ggtggcgtgg
     6841 gtgatcgccg gtcgggtcct ggcaccgctg cgccagctcg cgaccggggc cgcgtcgatc
     6901 cgggaggacc agctcgacca gcgcatcccg gtcgagggca cgggtgagct ggccgcgctg
     6961 agccggtcct tcaacgacat gctcgatcgg gtcgaggggg ccttcgccgc gcagcgctcg
     7021 ttcctcgacg atgccggtca cgagctgcgg acgccgatca cggtcatccg cgggcacctc
     7081 gagctggccg acccgtcggt gccgctgggg tcggacacgc gcgccctggt cttcgacgag
     7141 ctcgatcgca tggcccggat cgtggaggac ctcctgctca tcgccaaggc cgagcgaccc
     7201 gacttcgtcg tccccggccc cacggacatc gccgacctgg tgcgcgacat cgtgcagaag
     7261 gctcggccgc tggccgaacg ggcctgggag ctgcgcaacg acgcgctcgt cgttgccgag
     7321 gtggaccggg agcgggtgac ccaggcgctg atgaacctgg cgcgcaacgc catgcagcac
     7381 acgagcgagg gcgaccggat cacggtctgg tccgccctga gcgacgaccg cctcgagctg
     7441 ggcgtcaccg acacgggcga gggcgtcgcc cccgacgacc gggagcggat cttcgagcgc
     7501 ttcgcccgcg gttcgagcgc ccaacgcacg cgcagcgacg gcgccggcct gggcctcgcc
     7561 atcgctcggg cgatcgccca tgcccacgac gggcacctcg agctgcgcga cactccgggc
     7621 ggaggcgcca cattcgtgct gagcctgccc cgacccgccg aacccgatgc accgcccacg
     7681 gaggagccca tgtggcccgc atcctgatcg tggaggacga ggaccggatc gtgtcgttcc
     7741 tcaccaaggg cctcgaggcc aacgggcact cgaccatggc ggtcggcgac ggcatcaccg
     7801 cggctgcggt cgccgacgac ggcgacttcg acctgatgat cctcgacgtc gggcttccgg
     7861 ggaaggacgg cttcgccgtg ctcgaggaga tccgcgggcg cggcgtgcgc atgccggtca
     7921 tcgtgctcac cgcccgcgac gccacctccg acaccgtcac cggcttcgat gcgggcgccg
     7981 acgactacgt caccaagccg ttccgcttcg aggagctgct ggcccgcgtc cgggcccgcc
     8041 tgcgcgagca ggggaccacc gagcagacgc tgctggaggc cggcacggtc gtgctcgacc
     8101 tccgccggcg caccgtgtcg gtggacggtc ggagcgtcga gctgtcgtct cgcgagttcg
     8161 cgctcgccga gacgctgttc cgccatcccg gtcaggtgct gtcacgggag cagctgctgt
     8221 cgatggtctg gggctacgac ttcgaaccgg agtcgaacat cgtcgacgtc tacatcggct
     8281 acctgcgacg caagctcggc gccgagcgca tcgagacggt ccgggggatg ggctaccggc
     8341 tgcgcgccgg gtagccctac cgggcctcgg cggtgccggg cggcgcgacg gccacccagg
     8401 tcggcccggg cgtgagcagg accggctggc cgtcgggacc ggtgtaggtc gtgacgtcgc
     8461 ccacggccgg acgggaccag cgggcctcga tgcggcggcc ggcgctgaag acccaggcgt
     8521 cgccctcgcc caccagctgg gcctccacga tggggaagcc ggaggcgtcg cagcactgga
     8581 cctctgcgta gggggtgaac tggaccacga cgttgggtgg tgcgactcgg gcgccggcgg
     8641 cgtccacgtg cgccgagtcc cgctgggtcc gggcccagcc ggtgccgtcc cactcgtagt
     8701 cgaccggtgc gctgcccggg ccgccgccga acacgatgcg cacgccggtg gccgggtccc
     8761 cggtgggctc ggcgtcggcc ggtcggtact ggaagagcgc cggtggctgg cgctgctcgc
     8821 tggcggcacc gccgtacagc gcgccggtgg agctcatcag gttgtacggg gccggccgat
     8881 cgccggcccg gtagtacgcc ccgctgttga cgtcgaggcc gacgtcagcg accggcgcct
     8941 cccgcacggc gccgacgacc ccggcgttgg cgccggacca ggcgaacagc ggccggttga
     9001 gcggctccat caggagcagg tcggtggttc gggccgagcg cacggggccg accgggtcgg
     9061 cgtcctggga gtggaacagg gccaggaagc gggtgacgct gccctcgacc atctcctcga
     9121 acacgatgtc ggcctggttg atgccggcct gcggtcgccc gccgcgaccc gtgcggggct
     9181 cggcgttgtc gatcttcacc gccagcacgg gtcgctgggc ccgggccggg tcgtcgatcg
     9241 gctggcccgt cagcgggtag ccgggctcgg gcggggccgt cgtcgaggtc gtcgtggtgg
     9301 tcgtggtggt cgtgacggcc cgctcggcgg gctcctcgtc gtcaccgccg ctgagggcga
     9361 tgaccgcgat cacggcgacg atgacggcga gcacaccgcc gacgatcgcc cactggcggg
     9421 gggagagctg gggcacctgc gcgacggtag cgacgggggt gcggggtgtt cgtgatcggt
     9481 gggggcgggt acggatccgc catgtccgat cagtccaacg aacaacagac cggcgacctc
     9541 gacgagctgc tcggcctcga cgctgccagc gagctgctgg gtgtccccgt cgagcaggtc
     9601 cgggccatgg ccgaccaggg cgtcatcacg cgtcacgacc gggacggcgg ccccggcttc
     9661 cggcgggccg agctgatcgc ggcccgcgac ctcggcggct gacccgggtc agctcgcggt
     9721 ctgccgctcg tgcatgcggg tgagcaggct gcggatggtg tccacgtcct gggccccggg
     9781 gatcatgaac gcgtcctcga tgacgaaggc cgggacgccg gtgatctggc ggtcgacggc
     9841 gccctcgaga tcggctgcca ccgcgtcccg cccggtgccg gtggcgacgg cttcccccgc
     9901 cagctcgcga tcggcgccga catccaccgc ccacgtcacc aggttggcgg cgtcggcgat
     9961 gttggcgcct tcggtgaagt aggcgcggaa caaccggtcg tgcagctgcg ccgccgtgtc
    10021 cgggccgtgc tcggactcga cccacgcgac cagcgacagt gccgggaggc tcgtgacccg
    10081 ctgggcgagg tcgaaccggt agtcgatgcc cacctcctgg ccgaggggga cgaggcgacc
    10141 ggtcatgccc tggaacgcgc cgggcccgta cttgcgatcg atcgccgtcg ccaggtcctt
    10201 gggctcggcc gtcgccgtcg gatcgagctg gaaggcccgc caccggacct ccacctcgtc
    10261 tgcgaagtcg aggccctcga gagccacctc gagtcgccgc ttcccgaggt agcaccacgg
    10321 acagacgacg tcggaccaca catcgatgcg catgcctgca cccaaccaca ggtggggagc
    10381 gggtgttccc cgctcagccg gcgacctcga gggccccctc catcccggcg tcgaagtgcc
    10441 cttcgagcgc gcagatcacc tggtaggtgc cggcctcgtc gaccgtgaac gactcgctgg
    10501 tggactcccc gggcgggatc gcctccacct cgaagagcac gatctcctcg ctgaagtccg
    10561 cttcggactc gatctcctgc ccgagggcca gcactgccca ctcgtgctcg atgttgccgg
    10621 cgttctcgaa gtcgatggtg atctcctcgt ccaccgggac ggtccaggcg tccggatcga
    10681 actcgaagtc ggcggcctcg gcggcgatcg acgtcgatgc gtcggacgcc gccccaccgg
    10741 aatcgtcgtc gtccccgcag ccgccgagga ccaagagtcc ggccgtcatc cccacagcca
    10801 acacgcacgg tcgtcgcatg gcggatctcc gatccggtcg ggtcatcatc aaccctcgtc
    10861 catcgttctt gatacaccca gccagggtca cgagcaacga tctctctgcc gccagtcggg
    10921 atcttttggc ttcggcgtcg cgttccggac aacgttgccc catggtcgag accacccccc
    10981 gccacgactg gaccaccgac gacgcgctgg cactgctcga gcgccccttc cacgatctgc
    11041 tcctcgacgc ccagcaggtc caccgggccg gcttccggcc cgacgtggtc gagggggcga
    11101 tcctgctctc gatcaagacg ggcgcctgcc ccgaggactg cgcatactgc ccgcagtcgg
    11161 cgaagtggaa caccgacctc gagcccgagc gcctgatgga gaccgacgag atcgtggccg
    11221 ccgcgaccaa cgcgaaggcg tccggtgcgt cccggttctg catgggtgcc gcctggcgat
    11281 cgcccaacga ccgccaggtc gagcgggtcg ccgaagcggt ggctgcggtc ggcgagctcg
    11341 gcatggagac ctgcgtgacc ctcgggatgc tcaccgacgc gcaagcccaa cgcctcgccg
    11401 acgccggcct cgactactac aaccacaacc tggacacgtc gccggacttc tacggcgaga
    11461 tcatcaccac ccgcacctac caggaccgcc tcgacaccat cggtcacgtg cgagaggccg
    11521 gcgtgcgcct ctgctgcggc ggcatcgtcg gcatgggtga gagccgccgc cagcgggccg
    11581 gcctgctcgc ccagctggcc cagctcgatc cccatccgga gagcgtgccg atcaacctgc
    11641 tggtgcaggt gccgggcacg ccgctgcatg gcacggacga gctcgacggc ctcgagctgg
    11701 tccgcaccat cgccgccgcc cggatcatga tgcccggctc ggtggtccgc ctgtctgccg
    11761 gtcgcagcca gatgagcgac gagctgcagg cgctctgcat gcacgccggc gccgccagca
    11821 tcttcctcgg cgacaagctg ctgaccaccg gcaacccggg cgacgacgcc gatgccgacc
    11881 tgctcgagcg cctcgacacg tcgctggccg aggcgtccct cctcgactga cccggtacct
    11941 tcggcgccgc atgaggaccg actggaaccc gctgctgcgc gaccagttcg aggagcccta
    12001 ctggcacgag ctgcaggcct tcgtccgtga cgagcgggcg cgccacgagg tctacccgcc
    12061 ccacgacgag gtgttcgccg ccctccacct caccccgtac gcggacgtga aggtgctgat
    12121 cctcggccag gacccgtacc acggccccgg ccaggcccac gggctgtgct tctccgtgcg
    12181 cgacgggatc gacatcccgc cgtcgctcgt caacatccat gccgagctcg aggccgacct
    12241 cggctgcccc cggccgggcc acgggaacct cgagcactgg gcccgccagg gggtgctcct
    12301 gctcaacacg accctgacgg tgcgcgcccg gcaggcggcg tcgcaccagc ggaaggggtg
    12361 ggagcgcttc accgaccgcg tcgtcgaggt cgtcgacgcc aagcccgagc gggtggtctt
    12421 cgtcctgtgg ggtgcgtcgg cccggcggaa ggcggcgcag atcgacacgt cgaagcacac
    12481 gatcatcgag tcgccgcacc cgtctccgct gtcggcgcac aaggggttct tcggcacccg
    12541 cccgttcagc cgggccaacg cggcgctggt ggaggccggg cgcgacccga tcgactggtg
    12601 catcccgtga ccctgctggc cgatgtcgtc gccgccgccg atgccgtcgg cgcgacgacg
    12661 aagcgcaccg ccaagcgcga ggtcctggcc gagctgttcg ccgggctcgc acccgacgag
    12721 gtcccggtcg tggtcgggtt cctcctcggc gaggtgcgac agggccgcat cggcgtgggt
    12781 tgggcggcga tccgggatct ggactgcccg gcgggcgacg cggccagcct cagcgtcgac
    12841 gaggtcgacc gagcggtgtc ggtgctggcg agcaccaccg gacccggctc gcaggcggcg
    12901 cggcgatcgc agctcgtggc gctcttctcc cgcgcgaccg cgcccgaggc ggacttcctc
    12961 ggacgcctgt tcgtcggcga gatgcgcacg ggcgcgctgg ccggcgtgat caccgatgcc
    13021 gtcgccgcgg cagcgggcat cccggcagcc acggtgcgac gggcggtgat gctggcgggc
    13081 gacctcggtg cggtcgcccg catcgccctg accgagggac gcgaggcggt ggaggcggtc
    13141 ggtctcgagc cgctccgggc cgtccagccg atgctggcat cgaccagcga gtcggtggcc
    13201 gcagccatcg acgaggtcgg cgaggccagc gtcgagtgga agctcgacgg tgcccgcatc
    13261 caggtccacc ggtccggtga cgaggtgcgg atctacacgc ggaacctcaa cgacgtgacc
    13321 ggacggctcc ccgaggtggt cgaggtcgtg cggagcttcg acagcacctc cttcgtcctc
    13381 gacggcgagg tcctcggctt catgggtccg gaccaggcgc cgcaggcgtt ccaggacacc
    13441 atgagccggt tcggtcgcgg cgccggcgtg ggtgccctcg tgccgcagtt cttcgacgtg
    13501 ctccacctgg acgatgccga ccagatcgat cagccgctgc gcgtccggcg cgagcggctg
    13561 gccgccctcg tgggccccca cctcatcccc ggcaccacga ccgatgaccc ggacgtcggc
    13621 gagggcgtgc tcgaggacgc gctggcccac gggcacgagg gcgtcatggt caaggccgcc
    13681 gactcgtcgt acgaggcggg ccgccggggc aagtcgtggc gcaaggtcaa gcccgtgcac
    13741 acgctggatc tcgtggtgct cgcggtggag tgggggagcg gccgccggaa gggctggctg
    13801 tcgaacatcc acctcggcgc gctcgacccg gccaccggag agccggtcat ggtcggcaag
    13861 accttcaagg gcatgaccga cgagatgctg gcgtggcaga cggcgcggtt ccaggagctg
    13921 gagacccacc gctcgggcca caccgtgcac gtccgccccg agcaggtcgt cgagatcgcg
    13981 ctcgacgggg cgcagacctc gagccgctac ccgggtgggg tcgccctgcg cttcgcccgc
    14041 gtcgtccggt accgcgacga caagacggcc gccgaagcgg acacgctgaa cgccgtgcgc
    14101 tccctgctcg ccggtccggc catcccatga cgacgtcgct gctgtggttc cggcgtgacc
    14161 tccgcctgtc cgacaacccc gcgctggtcg aggccatccg gagcgccgat gacgtggtgg
    14221 gcgtgttctg cctggacccg gcgctcctcg gtcccgccgg cgacgtccgt cgccaccacc
    14281 tgttccggac cctggccgcc ctcgaccagt cgatggacgg gaacctggtc gtgcgcaccg
    14341 gcgacccgat cgcagtgatc ccggagctgg ccgtcgaggt cgaggcggca tcggtgcacc
    14401 tggccgagga cttcggaccc tacgggcgtc ggcgtgacga cgccgtcgcc gagcgcgtga
    14461 ccggcgccgg catggagctg gtccgcaccg gttcggccta cgccgtgcct cccgggtcga
    14521 tccagaacaa gtcgggcacc ccctacaagg tgttcacccc gttctcgcga gcgtggcggg
    14581 agcacggctg gccggacccg gtccggcgcc cctccgcgat cccgtggcgg tcggcgccga
    14641 gcgacggcat ccccgacctc ccggcgctgg gcgacgtgga gctgccacgc gccggcgagg
    14701 aagccgccgg caaccgcctg gaccacttcc tgcgggccaa ggtcgaggac tacgacgagg
    14761 cccgggacct gcccggtgtc gacgccacgt cgcgcctgtc acccgacctg aagtggggca
    14821 cgctgcaccc acgacagatc ctggcccgcc tcggcccgtc gaagggggcg cagaccttcg
    14881 ccacggagct gtgctggcgg gagttctacg ccgacgtcct cctccaccgc ccggaatcgg
    14941 ctcgggagtc gatggacgac aaggtcggcg ccatgccgtg ggaccggggc gccggtgccg
    15001 acgacctgtt cgaggcctgg acccgggggt gcaccggctt cccgatcgtc gacgccggca
    15061 tgcgccagct gctcgccgag ggctggatgc acaaccgggt ccgcatgatc acggccagct
    15121 tcctggtcaa ggacctgcac ctggactggc agcgcggcgc ccggtggttc atgcagcacc
    15181 tggtcgacgg cgacctcgcc tccaaccagc acggctggca gtggaccgcc ggcaccggca
    15241 ccgacgccgc cccgttcttc cggatcttca acccgacgac gcaggggcag cgcttcgacc
    15301 cggacggcac ctacgtgcgc cggtggatcc ccgagctgag cggggtgccg tcgaagtggg
    15361 tccacacccc ggccgatgct ccggacgggc tgcctgcggg ctacccggag ccgatcgtgg
    15421 atcaccggga ggaacgggag gagtcgctgc ggaggtatgg agcgacccgg tagggaatga
    15481 tcccctgatg gccatgatcg caccccagga cctcctcttc ctcctgatgg aggcccgaga
    15541 gaagccggcc cacgtcggcg ggctcgccct gttcgagacg cccgacgacg ccgacgagca
    15601 gtacctctac gagctgtacc agtcgtccat cgcctcggac gcacccgtgc ggtcgctgtt
    15661 ccagcggcgg gtcagccgtc cgggcctcgc cgggtactcc tggacccgcg acgaccagtt
    15721 cgacatccag taccacctgc gccacagcgc cctcccggtc ccgggccgca tccgcgagct
    15781 gctgaccgtc gtgtcccggc tccacggatc gctcctcgat cggaaccgcc cgctgtggga
    15841 ggcccacctc atcgagggcc tcgccgacgg ccggttcgcg ctctacacca agatccacca
    15901 cgccctgtgc gacggcgtca cggcgatgaa gctgctcgag tcgtcgatga gcccggaccc
    15961 gaccgagcgg gggatgcccc agccgtggga gcgtcgcccg cgcggctcga tgttcgacga
    16021 gcccgatccc gacgaggtgg cgccgccgtc acgacctgcc gcgcaggacg acacctggac
    16081 cgccgtgctc agcgacgggc tgcgcaagct cggctcgagc gtcaccgagt ccctcggggg
    16141 cgtggtcggg gcgaccgacg gcatcggcca cttgctcagc agcttccgcg accagtcgct
    16201 gtcgtcgccg ctgcgggcgc cccgctccat gctgaacgtg ccgatcacgg gggcgcgccg
    16261 cttcgccgcc gactcgtggt ccatcgaccg catccgcgcc gccgctgcgc cccacggggc
    16321 aacggtgaac gacatggtgc tggccatgag cgccggcgcg ctgcggacct acctgctcga
    16381 tcagggagcg ctgcccgacc agccgctggt ggcgatggtc ccggtgtcgc tgaagacccg
    16441 cggtgccgac gagcgcagcg gcaacgctgt gggcgccatc ctctgctcgc tcggtaccca
    16501 cctggactcc gcaccggcgc gcctcgccca catccaggag tcggtcaacc agagcaaggc
    16561 ccgcctcgag ggcctctcgc cgggccagat cctggcgctc agcgcgctga acgtggcccc
    16621 gctggcgctg aacatgagcc ccctcggccg aatgccgctg gccgaggagc gcatccgtcc
    16681 gccgttcaac gtcgtgatct ccaacgtgcc cggtcccaag gagacgctct actggaacgg
    16741 tgcccggctc tccgggtcct atccgatgtc gctgctcgtc gacggccagg cgctgaacat
    16801 cacgacctcc agctacgccg gcaacctcga cttcggcatc ctcggctgcc gccgcaccgt
    16861 gccctcgatg cagcgcctgc tgcggtacct cgacgacgag ctggtggccc tggaacgggg
    16921 ctgagccctc gagggccgac gagcggctag cagtcggtaa catcgagtgc cagctggacc
    16981 gctgccgtgc cgggaggtga ccacgtgctc gacgatcgga aggcctcgat cctgcgggcc
    17041 gtcgtgcagg agtacatcga gaccgcacag cccgtcggtt ccgggcgggt gtcggccgcg
    17101 ccgggcgtcg acgtgagctc ggccaccgtc cgcaacgaga tggcggtgct cgagaacgag
    17161 gggtacctgg cccagcccca caccagcgcc ggtcggatcc cgaccgagaa gggctaccgc
    17221 tacttcgtcg acaccatcgg cgcaccgggt gcgctgcggg gtgagcaggc caagcaggtg
    17281 aaggccttct tcgcccgggc ccacggcgag ctcgaggaga tgttcgagtc cacgagccgc
    17341 ctgctgtccg ggctcaccgc ctggaccggt gtggtcgtgc ccccatcggc cgccgacgtg
    17401 gcgatccgct ccgtccaggt cgtcgggctg gcccccagca ccgccctcgt cgtcaccgtg
    17461 ctcgccgacg cgatggtcga gaagcacagc gtcgagctgc cgggcgatgt cggcgacgac
    17521 gtcgtggccc gtgcctcgct gcacctcgcg tcggagctga ccggcaccat gcggggcgcg
    17581 gtgccgccgg acgtggcccc cacgggcgac ccggacaccg acggggtcgt gggtgccgtc
    17641 gtcgaggtcc tgcggcgcac cacgccggcc gacacggccg acgaggtgtt cgtcggcggc
    17701 gcgtcgcgcg tcgcctctgc gttcgatgcc gtcgagaccg tccagcaggt cctgagcgtg
    17761 ctcgaggagc agctcgtcgt ggtgacgctc ctgcgcgacg tcgtcgaccg cggcctgcag
    17821 gtggccatcg gcaccgagac cggcatggag accctggccg acgccgcgct cgtcgtggcg
    17881 ccctacaccg tcgacgggga gcgggccggc tcgatcggcg tcctcggccc ggcacgaatg
    17941 aactaccccg aggcaatggc cgccgtcgcc gcggtgagcc gccgcctcgg ccaccacctc
    18001 accgaaggct gaccggagac cccctcatgg ccgacctgta cgacctcctc ggcgtctcgc
    18061 gcgatgcctc ccaggacgag atcaagcggg cataccgcgc caaggcgcgc gagctgcacc
    18121 ccgacgccaa ccccgacgac cccgacgccg agcaccactt caaggaggtc gcccaggcgt
    18181 gggaggtcct gcgcgacccc gagaagcggg cccgctacga ccggttcggc gatgcgggca
    18241 tcggcggcgc cggccagccc ggcgccgggg accccttcgc cggcttcggt ggcgtcgggg
    18301 acatcttcga tgccttcttc ggcgggcaga gccccttcgg cggggctggc ggtggacggt
    18361 ccggcccgat gcgcgggccc gacctcgagg tcgtcaccga gctcgacttc accgaggcgg
    18421 tcttcggtgc ccagcacaag gtcgacgtcc gcaccgccgt ggcgtgcgag gtctgcgacg
    18481 ccaccggtgc cacgccgggg acctcggcca cgacgtgcgc ggagtgcggc ggtgccggcc
    18541 aggtccggcg cgtgcgccag tcgctgctcg gccagatggt caccaccgcg gcctgccccc
    18601 ggtgcaacgg cgccggtgag accatcgccg acccgtgcgg caactgcgcc ggcgagggcc
    18661 gcaccctgca ggaccgctcc ttcaccgtcg acatcccgcc gggcgtcgat gccgcctcca
    18721 ccctgcgcct gcagggccgc ggcgccgccg gcccgcgggg cggcccgccg ggcgacctct
    18781 acgtgcactt ccgcgtccgg ccccacgacg tgttccggcg cgacggggtc gatctccacg
    18841 ccgacctgca catccccgtg acccaggcgg tgctcggcgc cgaggtcgac ttcgagaccc
    18901 tcgacggcac cgagacgatc acgatcccgc cggtgacccc caccggccac gtgttccgct
    18961 tccggggcaa gggcgtcccg gtggtcaacc gcggcgggcg gggcgacctg cacctgcacg
    19021 tcgtcatcga cctgccccgc gacctcaccg aagagcagga ggagctggtc cggcgcctgg
    19081 ccgagctccg gggtgaaccg gtggccgagg agtcccgcgg cttcttcacc aagctgcgca
    19141 ccgccttcaa gtgagcccgg agccgcccga cggcactgca cctcacgtcc tcgtcgacga
    19201 tgtcgaggtg cccgtcctcg acgaggcggc gctccaccac ctgtccaagg tccgtcgcct
    19261 ccgggacggc gacgccctga ccgtcaccga cgggcgcggc gcgtggcgcg ccggtcggtt
    19321 ccgggggcgc gccgaggtcg agtcggacgg cccggtcgtg gaggtgccgg cgccgaatcc
    19381 cgaggtgacg gtggccttcg ccctgaccaa gtccgacaag cccgacctgg tggtgcagaa
    19441 gctcaccgag ctcggcgtcg accggatcgt ccccttccgt gccgatcgct ccgtcgtgcg
    19501 gtgggacgag gccaaggccg cccgccagca cgagcggctg gtcgccatcg cccggtccgc
    19561 ctgcgagcag tcgcaccggt gctggctccc caccgtggaa ccggtgacgg acgtggccga
    19621 cctggccgcg cgcggtgcgg tccgcctcga ccgtgggccg gtggccgtct ccctcgaccg
    19681 cccggtcgtg gccgtcggtc ccgagggcgg ctggtccgat tccgagcgtg ctgccctccc
    19741 cgacacggcc ggcctgggtc cgcacgtcct gcgcgccgag accgccgcca ttaccgccgg
    19801 tgcgctgctc ggtgcgatgc gtagcggctg gggcgggtga gggtggccgc gaccggacct
    19861 ggcggaaatt gcgccgattg tctgggtggc gagggttgtg cggagcgtgg tcgatgccta
    19921 cgttgagcgt tgacaacggg tggggatcgg agctggagat ggcgatggaa gacctggacg
    19981 aggcgggact ggcgtacagc cgcaaggtgg gcgagcgact gcgcgccatc cgccggcaga
    20041 agcggctcag cctgcacgac gtggagtcgg cctcggactc ggagttcaag gcgtcggtgc
    20101 tcggcgccta cgagcgcggt gagcgctcga tctcggtccc gcgcctgcac cgcctggccg
    20161 gcttctacga cgtccccgtc gaccagctgc tccccaccga cgaggagggc gaggtcatcg
    20221 acctcaccga ccgggcgctg gcccgccagg ccgagaacca cctcgtgctc gacctgcaga
    20281 tgctgtccga gctcggcggc cccgagggcg ccatgctcaa ccgctacatc cgcaccatcc
    20341 agatccagcg ccaggacttc aacggccgga tgctcacgat ccgcaaggac gacgtgcggg
    20401 ccatcgcctg catcctcggt gtggccaccg accgcacgat ggaccgcctc gaccagctcg
    20461 gcctctgccg ggtcggggcg tcgcgctgag ccgagcccct gagaccttcg gcgcctacct
    20521 ccacatcccg ttctgctcga cgcgctgcga ctactgcgcg ttcgcgacct ggaccgaccg
    20581 cgggcacctg gtcgaccggt acctggccgg cctcgccacc gaggtcgacc gggccgtcgc
    20641 tgacggcctg ccggcgctga cgtcggtctt cgtggggggc ggcaccccga cgctcgtgcc
    20701 cgccgacggc ctcgtcgacg tgctgtcccg gctgccgctg gcgcccggcg ccgaggtcac
    20761 ggtcgaggcc aacccggact cggtcacggc cgagtcgctg cgcacctacc ggcggggcgg
    20821 cgtgacccgc atgtccttcg gcgtgcagtc gatgcgcgag cacgtgctcg ccgccctcgg
    20881 gcgcacccac gatcccgaca acgtgcgcca agcggtcgcc tggacgcggg aggccgggtt
    20941 cgagacgttc aacctcgacc tgatcatggg cgccgccggc gagtccgtcg aggactgggc
    21001 gtcgagcatc gaggacgtgc tcgagctcga cccgccccac gtgtcggcct acgggctcac
    21061 ggtcgagccc ggcacgcccc tggctgcgga tccggaccgc taccccgacg acgacgacca
    21121 ggccgacaag tacctgctca ccgacgagcg cctcggcgcc gccgggctgc gctggtacga
    21181 ggtgtcgaac tgggcccgtc ccggtcacga gtgccggcac aaccggctgt actgggaggc
    21241 gggggagtac ctcgggttcg gctgcgccgc ccacagccac cgagccggac gccgctggtg
    21301 gaacctgcgc acgcccgagc gctacctcga cgcggtcgaa gccggcacct ccaccgaggc
    21361 cgccggcgag gacctcgacg agggcacccg ccgcctcgag accctccagc tcgccctccg
    21421 gacccgtgag ggcgtgccgg tcgccgcgct cgacctcgac gacccctcgc tcgagggcct
    21481 ggtcgacgtg gtgggggacc gggtggtgct cacgccggag ggacgcctgc tcgccaacga
    21541 ggtcgcggtg cggctgcgga cggatgtggc cgtcggcgtc ggcccgcgtg ctcagcccgg
    21601 cggagccgag gggaccggcg gcacctcgaa gacctgaccg ggcaggatcg catccgggtc
    21661 ggccggatcg gtgaggcggc cgcggttgac ctcgacgagg acgcgccaga acgggtccac
    21721 ctcggcatcg gacggcggac gccgccacgc gtcgaccagc agctcctcgg cgatcgacca
    21781 gaaggactcg ccgggggcga cggtccacgt cgacggtcgc ggggtcggcg tcatgaccgc
    21841 cgtgccgtgg tccccggagg gtgacaggag cgggcgcatc gttgcggtcc cgccagccgc
    21901 ttcgacgccg ccggcgcgca ccaggggcgc ctcggccacg ggcgtcatcg tggcgacacc
    21961 gggtgcgaca ccctcgtcgc cgccgccgac cgtggacgcg gctgcgagac cgatgcccag
    22021 gccggcgtgc acgagccggc gagcggcggg caccgccaag gcatcggcga ggcgctgacc
    22081 gccgacggcg cgcagcgcac ccgaggcgac gtgcagcgcg gacagcgcca gcacgtacca
    22141 gacccacacc tcggcgagga ggcggaccag ggcgacggcg gccgcggcgg gtccgcgccc
    22201 gtccacccag gtcgtcagct ggtcgaagga gccgagcggt ggcgccgcca gctccccccg
    22261 ggaggcgagg cgcaggctga cgccggcggc cagcagcccg gcgagccaca gcagcgaccg
    22321 agcggcccga cgggtcactc ggggacctcg accgtccggc tcccgtcggg tgtcgagagc
    22381 acgggggcgg gacactcccc ggagctggcg gcgatggcgg tggcgccagg cacggtggtg
    22441 agggcctcga cctcgacggg ctcgtcgagg cgccactcgg ggaacacgaa cacctcgccg
    22501 gtgtcgggcc ggagcagggc cggggtggcg atgccgtcgc agcgccagtc gccgagggcg
    22561 accaggtcgc cgtcctgacc cagctccacg gcgacggtgc cgatctgggc ggtgcggccg
    22621 tcgaggacga cctcgtccgg gcacccgtcg ccgtcgacgt cgggaccgac gggttccggg
    22681 agggtgcacc gctccggcca ggggcctgcc tccggcgcag cggtggtgct ggtgctgctg
    22741 gtggtctggc cgggcgcggt ggtggtcgtg gtcgtggcat cggtggccga caccgggtcg
    22801 gagccgagcg gccgcacggc tcgggcgccg gcgaccccgc cgaccacgcc gatggcgagc
    22861 acgagcagca ggagcgcggc gcggacgagc gggagccggc ggcgctgctc ctcgtgctcg
    22921 cgccggtcgg ggaggggcac gctcggaccc ggtgccggag ggggtgtcgc ctcgtgttgg
    22981 atccgggctc gctcccgggg tccctcgtcg ggcggggtga agaccgggag cggtccggtg
    23041 acggcctcgg gggcggcggc cagcgccgcc cagtcctcgt cggtgccgtc gtcgagctgg
    23101 tcgagggagc cgaggccggc gtaggggccg gcgtcggcct cgagctcgtc ggcgttgcgg
    23161 tcgtcgtcgg ccaccgcggc gagccagctc aggtcctcgt cggtccagcc caggtcagcg
    23221 gtggggtcga cgtctgccgg cagctcggcg aaccacggtg ccgccggacg cgcggcactc
    23281 ggggtcggca gggagcactc gggcacggcg gcgtggaccg ccttggccag ctgccgggcg
    23341 gtgaggcgct ggtcccggcg ctccctcgtc gcctggacga cgatggcgtc gaggtccttg
    23401 cgggcttgac gctgccgggc ggcgcggcgg accccgctcg gcgggttcgc ccatggcagg
    23461 tcggcgccgg tgcgcagcag gctgcccagc aggcgcccga tggcggccac gtcgtccgca
    23521 gcgccgtcgt cggtgcggtc ggccgccatc ccgaacccgc acaggcgcgg ccggccgcgg
    23581 tcgtcgagga ggacgtgggc ggcctcgagg tcgccgtgga ccacgccgag gcggtgcagc
    23641 gcgtgcacga cgtcggcgac ggcgcacacg atgccagcgg cgtggtcgag ggggaacggc
    23701 ccgagccggg ccagcgcggt gccggcgtgg cggaggcgga tggcccgctc ctcgacggcg
    23761 agcagctcga ccaccccggg gtgccggcag cgggccagca tcgctgcctc gcgggtgagg
    23821 cgggccacgt cgccgtcgct gcgcgcctcc ttgaccacga ccaggccgcc gtcggcttcc
    23881 tcggtgcgca cagcgatgac catcggtccg gacgctaccg gaagggcatg agatgcatca
    23941 aggttctgtt gcggggcgat gcacctcgcc gccgacccgg gccgagatcg tgaggacgtc
    24001 ccgggccggg tcgccgaggg cctggacgaa gaggtgctcc acggtccggc gcacggtgac
    24061 caccacgctg tcgccgtcgg gcaccgccga gacgagctcc aggtcgtcga tgccggccgc
    24121 agccacggcc tgcgcggcga tggcgtggac ccgatccggg tcgtagacga tctcgccgct
    24181 ggtgcgggcc gcgtcgaggt cgaagccggc gccggccgca tcgttcgcgg cgtcgaaggc
    24241 cacgttgtgg gcctggcgct ggcccaggta cacgatggcg gagtcgacgg cgatggcgcc
    24301 gagcagcagc acgatgagca ccgcgacggg catgagcatc aggaccgagc cgcgggtgtc
    24361 gcgagctcgg gcggtgctca ctggatgcac gacgccgccc cgtccacgcc cgagcgcgtc
    24421 gggtcgacca gctcaccgtg cgtggcctgc acgtcgaacg cgtcgccgaa gcccccgacg
    24481 aagggcaggc ggatggccgg gacctcgatc gagaccgtga cctcgatgcg ctcgcagcgc
    24541 acgaaggccg cccccggtgg tccgacctcg accgtcgcgt ggccggtgcg gccgtggtcg
    24601 tcgagggtgt cggtggcgat cgtggtggcg ccggctcgga gcgcgtcggc ggtggtggtc
    24661 tgcccggcgg cctcgacgac ccagcgcgcc gactcgcgag ccgcagcatc ggcggcgtac
    24721 ttggcatcga cgacgcccca gatgttgatg atcagcaggg cgccccccac gaagatcagc
    24781 agcgcgaagg ggagcacctc catgccgccg gcttgaccgc gctcgccccg gatcgttctg
    24841 gcagcggcca cggaacaggt cgggcggagg gggctcacgg tcctgccgcc tggaagacct
    24901 cgaggcgggc gcgcatcgtc cggctgaact cggtcgggct gtagcccgac cagcggaccg
    24961 ggagcaggat cggcagggct gcgtggacgg tcacggcgac cgtgtcgtcg cgcccgtcgc
    25021 cgtcggcgtc ggtgagcgcg aaggtgaagt cggcgtcatc gccgtagccg ccgaggcgct
    25081 gccggccctg cgctgcgtag gcggccatgc gagcccggtc cagcccagcc ccctcgttcg
    25141 ctgctcgctg ggcgaggtcg gacgccacgg cggtcgcagt ggtgcgtgca tggagggcga
    25201 agaggacctg cacggtgaag aggaggaaga ccaagaagac cgtgaagccg aatgcggtcg
    25261 cgaagatgcc ggtgcctcgg tcggcgaccg gcgtgcggga gggctctggc gacgggcagc
    25321 gcatcgctcg aggaccgggc ggtccgccgg atcagccgct gctgccgtcg gacccgatga
    25381 cgttcacctg gtcctccgtg ttctgggcgg tgtcgctcca gatgttgtcg aacgtcagcc
    25441 acatggctgc gccgagcagg gccatgatca agacggcgat ggcggtcgag atgacgcctt
    25501 cgccgcggtc accacgtcgg tgggcggtgc gggcagtggt gctcatggtt ctctcctggc
    25561 tgtggcggga ctaggaggcg ctgaacacgc gcagcgcctc gaggaacggg atggcgatga
    25621 agatggcgcc gggcacgagg gcggcgacgg tgacggggat ccagacctgc tggctgcgtc
    25681 gctcgatctg ctcgacgagg ccgcgctgga cctcgcgccg gatggagcgg gcctcctcgg
    25741 cgatgaggcg gccgaggtcc ccggcttctc ggttgagggc gagcacggcg acgagtcggg
    25801 tgagggcatc gacgtcggcg acggccgccc actcgcgcag ggcctccacc tcggtgacgc
    25861 cgtggcggac gcgtcccacg acgcggcgca ggtcctggcc gctggccccc tgaccgcggg
    25921 tggacaggcg ctggagggca gcgccgaggg agaacccggc cgacaggagc atcccgagct
    25981 gctcggagac gaccggcagc tcgaggagga gtcggcgctt gcgccggtcc gacgccatcg
    26041 ccagctgctg ctcgacgaac aggaacgaca gcgcgggtgc acccaccagg aacagcagga
    26101 ccacggccgc cggcgggccg atcgccaggg cggcgagggc gccgccgacc gcgccgaccg
    26161 cggtccagcc gagctggcgg acgcggaagt cggtggggtc gagctcgctg tgcacccgtt
    26221 ccaaccggac cgccagcgcc tcgttgatgc cgacgaggcg ggcgacgcgc tcaccgacgg
    26281 tgcgggcgag cggggcgatg acgtcggcga aggagtcgac cgagagcatg ccggggccgc
    26341 ctgcggacgt cgccagaccg ccgggttcgt agggccggag gcgatcggcg agggaacggc
    26401 gggcgaacca ccggtggcgg ctgagcacga gggtggcgcc ggcccagaac gccagggtgg
    26461 cgacgatggt gacgcgggtc atcggtcgaa cacccgctcc tcctcgggga cctgcaggat
    26521 ccgcccggcc cacagccagc agccggcgac gagcaggacc gcgaccgagg tcaggacctg
    26581 gccggtcgcg gactggaagg cctggcgccc gttgcccacc gacatgccgg ccagggccat
    26641 gccgaacggg gcgaagacga cgaagcgacg ggcgaagcgg gcgccggcct gcttggcccg
    26701 agcgtccttg cggccctggg tgtcctggat gcggtcctcg atgagggcct ccaggcggcg
    26761 gtcgaggtcg ctgcccccga ccgtgtgcgc gacgagcagc gtctcgcagg tggcgtccgc
    26821 ggttggatcg gccagcccgc ccttgagcac ggtgagggtg cgttcgaagt ccgtggagat
    26881 cagccactcc cggtgggccg catcgaacgc ggggaccatg tcgtccgggg cccgccggcc
    26941 gacctcgaac agcgcttggg ggatcgaccg gccgagcgag gaggtcagga tccggatctc
    27001 ctcgatcatc cgcggccagg cgtcctgggc ccgggcccgc cggttggcgc ggcgcacgcg
    27061 gtaggaggcc agcggcgcgg tcgcggctgc gaggccgacg gcgatcgccg gggcgacggc
    27121 cccgaagatc gcgaaggtca gcgcggcgcc gagcacgagc agcaggacca tgacggcggc
    27181 gaagtcgcgc aggcggacgt cacccaagcc ggcctggacg agccactcct gcaccacggg
    27241 tcgtcgatcc cggcggttgc cgacggccgg ggcgaccccg acgcctcgcc acccgaaggc
    27301 gacggcggtg tacaggtagt acacgccgag cgctgcggcg acgggcagga gcagggcggt
    27361 catcggtcct gtcgggttcg gtcgggaacg cccagcagcc cgcgcaggtc gaagcccgcc
    27421 tcctccaggg cccgggccgc ccggttcggc agcaggccgg tccactcgag gggttggtcc
    27481 cgccgggccc ggcggaacag gtcggtgacg gtgaactgcg tggcgtcgtg cccggcggcc
    27541 tggtcctcca cggcgatgat ctcggtgacc cgcggtccgc cggcctcgcg cgcggagtgc
    27601 accacgatgt cgatggactc tgacacgagg ctgttgagcg cggacatcgg gatctcgctc
    27661 gacgtgtcgg cgagctggca gatgaagcgc aacctcgaga gcgcttggcg ggcagagccg
    27721 gcgtgaatgg tggtgtagcc cttcacgccc gacgacagcg tgagcagcag gggcagtgcc
    27781 tcgcggtccc ggacctcgcc gacgatggcg acgtccggtg ccatccggag gaagccggcg
    27841 acgaggcggc gcaggtcgac ggctggccgg tcgggtcggg cggcgcgcgt ctgcatcgac
    27901 gcgacgttcg gcagcgggac gtcggcctcg aacacctcct cggcgacgac gacccgcagc
    27961 gcggggtcga gctcggcggc ggtgcaggac agcagcgtcg tcttgccgga accgggagcg
    28021 ccggcgaaga cgacggtgag cttggcccgg gcgcaggcac ggaggaactc ggccgcctgg
    28081 gcgctgagca tgtcgcgctc gaccagctgc tccagctcgc ggaacgcgac gcccgtgaac
    28141 ttcctgatgt tcaccatgac gtgcccgccg ctcgccacgt cgctgtgcac gatgtggagt
    28201 cgggcgccgt cgtcgagctg ggcgtcctgc agcccctccg tggggtcgag cttgcggtgg
    28261 gcacccgatg cgtcgtcgag gatcttcgtc agggtgcgga tgacgtgctc gtcgtcgtgg
    28321 aagacctcgt cgtggtagcc cgagggcccg cggtggcgct tcacgaagac ggcgtcgggc
    28381 gcgttgatca tcacctccca gacgtcgtcg tcggcgagga ggctctcgag cggcccgtag
    28441 ccgacgaggt tgcggtaggc ccgctccacc agcagctcgg ggtcggccag atcgtgggtg
    28501 cgcagtcccc ggcggtagtc ggcggaccaa cgggcgacgg cgtcggtgat gaggtcgcgc
    28561 atgcgtgcac gggcggcgtc gtcgtcgagg tcgaggtcga tccccttcgc ctgctcgagc
    28621 accgtgccct cgatgagctc gaggggggtg gcatcggtgt ggacggtgat cacccggcga
    28681 cctcctcgtc ccaggaaccg agcgatccgg cctggacggg aaccggctcg tcgaacccgg
    28741 gcaggtgggt gcgttcatcg acggcatcga gccgggcctg gacggcctgg ctgaccggtg
    28801 cgacgaacgc ctcgggcagc gcgaccccgt cccggagcgc gtcgtcgagc tgcttgcgct
    28861 ccgggaggaa caccggactg agcacgggtt ggccggggtt cgacgcggcc agcagctcgc
    28921 tgatggtccg gtggatctcg gctcgggcgt ggcggctgcg cggcgcccgg ttcacgaccg
    28981 ggacgatccg gtcggcgtcg acgccgttgg cgatgcagtc gcgcacgacg cgcaggagcg
    29041 aatgggcacc cttggtgccc gggttgccga cgacgacgac gacttcagcg aggccgatgg
    29101 tcgtccgggc gagcaggttg cggtcctcga cgtcggcgga tccggtgtcg gcttcgccct
    29161 ccaggtcgtc gtccacatct gcgaccacga gggtgaagct gcgtcggagt ccgtcgagcg
    29221 cgctgcgtag cgcccggggg cgcagcgccg tccagtcccg gtgacggcgc agcccgagca
    29281 gcagccggta ccggcgggcg acgatgtcga aggtgagcgc tcggacgtcc tcgaggctgg
    29341 ggacgccgct gcggtgggcc tcgaccagct cgagcacgcc cgggatcacg tcccgtgcgt
    29401 cgtgcagcat cgcctggtcg gcgtggagcg cgaggtcggc gagcaccacc gtgtcggcat
    29461 gggtcggatc ggccgctgca ccctgggcga cggccatggc gaccagggat cggccggtac
    29521 cgcctggtcc ggtgatcgcc accacccggg cgcggaaccc gatgtcgcgc cggtccggga
    29581 tctgcacctg cccggggaga tcgtcggccc gcccgatggg cctggcgtgt tcggacagga
    29641 ccgcctcgag ctcggtccgg ccgaagtcgc ttgggagcac gtggaccgcg ccgatcgtcg
    29701 accagttccg ggcggccccg tcgaccacga cgatgggggc gcacccgtgc tcgcgtgcca
    29761 gctcgagcag gtcacgatcg atgccgggca tgccaccgtc cacgaggagg gcggagaacg
    29821 gtcgaccgga ctcgaggcgg gcccgcacct cggcggcgga caccgtcttg acgaagtcca
    29881 tggccagctg ccccgacgtc gcccacctcg ccacgtcgct gaaccacggt gcgcggatcc
    29941 gggcgagacc cagggtcacg aaccgctcgg acatcaggcg tcctcgtccg ggtgggtgct
    30001 gcggccggcg accgaccggg gtcggtactc ccggccgacg tcttcgacct cggtcccgag
    30061 cgtggcgcgg accatcgtga tgtcgcccgc cgcctgggcg tgcaccagcg cttcgacctg
    30121 gtcgaagtcg ctgagggcca gacggaccac ggtcgtgtcg ctgccgccga ggccctcgcg
    30181 cgcaccgccc cccacctcga gcacccgggc atcgaccgcg atcagctggg tgacctcgct
    30241 gctccaggtg gagaacacgt cgacccgctc acccgcacgc agcccgccga ccgctcggct
    30301 ggtcgggagg ctgagggcga cctcgaggtg ctcgtcgccc tcgaccggaa cggcagtcgc
    30361 cgaggcgagc aggaactccc cggcgtcgac ggacgcgacg agctggcgac ccacagccgc
    30421 gtcgacggcc ccgaagagac cccgtacccc ggcgggcagc tccaccgaca cgagggccac
    30481 gtcgccggct cggacggtct ggcccggagc caggtcccgc gatgcgacca gcacctcgat
    30541 cgtgtcatcg gccgtcgcgt cggcgtaggc gacgaaggtg cccacggcgg cgagggccat
    30601 gagcaggccg ccgaccaccg cacgtgtcga gggcagcccg aggcgacgtt cgacgcgccg
    30661 cggcgtcggg tccggtccgg ccgtggccct cccgttccgc cgggtgaggg tgctggtcac
    30721 ttcgacccct ttcggtggga cggcccgctg ctcctgcccc cgtcgggtga caatcgggtt
    30781 gccgaggagc gcttgctgat ggagaacaca tgaaacacca tcacaacatg tcacatgagg
    30841 tcaacggggg acatctggaa ccatcgagat cagccgagat gctggatcgg ggtccaggag
    30901 gcaggtatgg tcggcgatgg gcccggtccg catgggccgt ccgccgacct cggaggtgcc
    30961 gatgaccgca tccgtcgaat ggctgtccac agccgacgcc gcgaaggcgc tcggcatcac
    31021 gccgcgcacg ctgtaccgct tcatcgatca gggagagctg gccgcgtacc ggttcgggcg
    31081 ggtcatccgg atcaagcagt ccgacgtgga tgccttcatc gagggtgctc gcatcgagcc
    31141 cgggtccctc gagcacctct acccggactc cggcggcaag cccgagggcg cggcactcga
    31201 ctccgaggtc tagggctcga ggaccaccac gtcgatcgcg ccgagcggga tccacaccgg
    31261 tgccggcggg tcgccgtcgg tccgcagccg caccacgtcg gtgccggcgc tgcgcagctc
    31321 gccgcggacg ccctgccccg agcgcgtgcg gatgagcacc tgggggcgat cggcggccag
    31381 cggacccacc accgcgccga gcgccacctc cacgaggacc gtccggtcac cggtgaccgg
    31441 gcgaccgccc ggctcggccc gcaccacctc gaccgcctcg gtgcgcacca gcacgtcctg
    31501 gtcgcgctcg ctgcgcacag cgaggaagtc ggcgccgatc ccgacgatgg tcccgcggat
    31561 ccggtggtca ccgacgtcga ggacgacggg gcggcctccc tcggccaggt cgaccagcgc
    31621 accgagggtg ctcgcttcct cctccgcctg gatccgcagc cagcgctggc gggttcgctg
    31681 gcgagccgcc tcgtcgacga gcgcgtccgc agcccagcgc tccaacgacc gggccagcgc
    31741 gtcggtgccg cccagcacac caggggctgc cgctcgtgcg gctgccggca ggtcgctgcc
    31801 gtcgtcgtgg tgcactgggt ctcctgcggg tcgggtgccg gtaggatccg gctgatcgtg
    31861 tccgacaccc aagtcgtcgt caacgtccct ggcaaccacc tcatggccgg tctgctcggc
    31921 cagcgggatg agctgctccg actcgtggag cgcgccttcc ccgacacggc catccacgcc
    31981 cggggcaacg agatcgccgt cgtgggcccc gaagcacccg ccgccggcaa ggtcttcgag
    32041 gagctcgtgc tgctcgtggc gtccgggcag ctgctcgaca ccgagagcgt ccagcgcgcc
    32101 atcgacatgg tcatccacga cgaacgcccg tcggagctga tggccaccga gatcctgcgc
    32161 tcggccaagg gcaacgccgt gcggcccaag accgcgggcc aacgccgcta catcgaggcc
    32221 atccgcgacc acgtcatcac cttcggtctc ggtcccgccg gcaccggcaa gtcctggctc
    32281 gccgtggcca tggccgtgca ggcgctgcac cgcaacgagg tcgagcgcat catcctcacc
    32341 cgcccggcgg tcgaggcggg ggagcgactc gggttcctgc ccggcgacct catggccaag
    32401 atcgacccct acctgcggcc cctctacgac gccctctacg acatggtcga ccccgagggc
    32461 gcccagcgcc tgctcgagcg caacctcgtc gaggtcgcgc cgctggcctt catgcggggc
    32521 cgcgcccagc ccgtcgaccg caaggtcctc acgcccaccg gctggcagga gatcggcacg
    32581 ctcgaggtcg gtgacctcgt catcggctcg gacggcatgc cgacccccgt gctcggcgtg
    32641 ttcccgcagg gtcgcaagga ggtcttccgg gtcgccaccc aggacggctc gtcgacgctg
    32701 gcgtgcggtg agcacctgtg gacggtgaag accctcgacg acaagaagca cggcaagcca
    32761 ggtcgcaccc tccagaccag cgacatggtc ggcaacctgc gccggcacca ccagcgcttc
    32821 tacgagctgc cgatgctgag cgccccggcc cggttcgagc cgcaggacgt cccgatggac
    32881 ccgtacgcgc tcgggctcct gctcggcgac gggtgcatca cgggcaagac cacgccgacg
    32941 ttcaccacgg ccgatcccga gctcgccgtc gcactcgaag a
//
LOCUS       DATISU010000135        10752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_611889, whole genome shotgun sequence.
ACCESSION   DATISU010000135 DATISU010000000
VERSION     DATISU010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 10752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10752
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611889"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VLR27_05625"
     CDS             complement(<1..890)
                     /locus_tag="VLR27_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSP02959.1"
                     /translation="MEVLDLLVLGSGVAGLSAAVRAADVHGMRVGVLTKAELHQATTR
                     WAQGGVAAVLGGDPDSTDLHLADTLGAGAGLCDLDAVRVLVDEGPVRVNELIALGAMF
                     DQDERGQYLLAREGGHSHARILHAGGAATGVEVERALVEAVQKTAAVLLERTFALDLL
                     VEGGRCAGVTAIDEHGERIEVRARNVLLATGGGGQLFSVTTNPLEATGDGMAMALRVG
                     AALADVEFVQFHPTALHHEAMPRPLLSEALRGHGAFLRDASGERFVDELQPRDVVSRA
                     ITARLQADRTDHVWLDATVL"
     gene            complement(970..1542)
                     /locus_tag="VLR27_05630"
     CDS             complement(970..1542)
                     /locus_tag="VLR27_05630"
                     /inference="COORDINATES: protein motif:HMM:NF037405.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HSP02960.1"
                     /translation="MPPIHIDGIPCTPPRRLAVDIGAVLGDTAYTTVIRELRRSHGVS
                     WKQLAAILELHSRRGRNGCGPLRRQLERYHGIDGIPDTTLEQVFLDDLLDAGFPLPTC
                     QHVVPQPHGDPFRLDFAWIPVRLDVEIDGPHHRTDPARRRDARRDAVLRRLGWKVLRF
                     DEEEVMYAPGGVLHEVRRTLEHLGGRTGRC"
     gene            1692..2567
                     /gene="nadC"
                     /locus_tag="VLR27_05635"
     CDS             1692..2567
                     /gene="nadC"
                     /locus_tag="VLR27_05635"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HSP02961.1"
                     /translation="MSDVPVEPPIHAVRAAVAAALAEDLTPLGDLSAALVPADATIDA
                     VFASRAAGVLAGTACATEAFAQLDPAVTVSWSHHDGDRIDAGTTIATVTGPLAPVLTG
                     ERTALNFLCHLSGIATLTRRFADAAAEGGDARIWDTRKTTPGLRSLEKAAVRAGGGAS
                     HRGNLSDWVMFKDNHLAAIGIEEAVRRARTAWPARTIHVEVDDLDGLRTALTAGCDAV
                     LLDNFTPDQAAEAVAIADGWAAEHGRRRPLLECSGGITVDTAAAYAAAGVDLLSTSQI
                     TQSAPALDIGLDVRG"
     gene            2569..3972
                     /locus_tag="VLR27_05640"
     CDS             2569..3972
                     /locus_tag="VLR27_05640"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HSP02962.1"
                     /translation="MGRAEGPWDLGGRTRRAVTPARLLARGAAAAAMAQARALRGDSI
                     QDIASDRRLVAAAEDIARELGEMKGAAMKIGQALSILDVGLIPEEYRTALAVLQSDAP
                     PMPYDAVREVVTAELGAPPEELFDFFSPRPMAAASIGQVHMAHLGDDELVVKVQYPGV
                     ADAVAADLRNAALLSALARLMQRMLAGLAGDVDVRALIDEVRDRVTDELDYRIEAANQ
                     LEFAARFAGDPEIDIPAVREDLSTRRVLTSEYVDAMRWSAALEQPAELRNKWGNVVSR
                     FSATSIYDHGVVNVDAHPGNFLFHEDGRVTFLDFGCVSRLTPDQLVRLRRLAAALMTG
                     DRDAVLDTFVDAGFLRSREGFDPEVLLGPLGSWVQAARGPQPFRYSREMLADIVAEAM
                     RVRVGVDELKLLQRLDVPREHVLLGRVSVGNEAILAHLEATIDFRDLYARHLAGALDI
                     DPEWPDASDEDGSGGVA"
     gene            3984..4796
                     /locus_tag="VLR27_05645"
     CDS             3984..4796
                     /locus_tag="VLR27_05645"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HSP02963.1"
                     /translation="MNLLAIDVGNTQTVIGLYDLESSVEPRDRVADSDLLDHWRIATN
                     VERTADEYALLFQEFLAFHGFGWTEDVQGFAVCSSVPRVTGALRRFSERYLGFDALVV
                     EPGVKTGMPILYDNPREVGSDRIADAVAAWDLYGGPTIVVDFGTATTFEAISAKGEYM
                     GGAIFPGIDISLDALFGRAAALRRVELVEPSNVIGRSTIESMQSGAVYGFTAVVDGMV
                     ERFQVELDQPCTVVATGGLSGLIAPLSSTIQHHEPWLTLHGLRIIHEKNPHV"
     gene            4789..6213
                     /gene="lysS"
                     /locus_tag="VLR27_05650"
     CDS             4789..6213
                     /gene="lysS"
                     /locus_tag="VLR27_05650"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HSP02964.1"
                     /translation="MSDDAPFDPPYRLDVTDRVGAILTEFDHLADGEETDRTVTVAGR
                     VMLLRDQGRLAFATLREGSTGDEIQLFALSKVLDGFDRFVKLNLGDWIGATGEVVRTK
                     RGELSIKVTSFVPLAQARRGFGDKFHGITDTDLRYRQRYADLWANPEAAVALRARSRT
                     ISLIRRWLEERDFVEVETPVLQHVASGAAATPFTTHHNALDVDLKLRIAPELFLKRLV
                     VGGFERVYEIGRVFRNEGISTRHNPEFTMLELYQAYADYTDMMALVEDLVAHLATELH
                     GTTTLTYDDRELDLSTPWRRASMVELIEDAIGVRLDVGQPLDEVRAVATEHGVEVEGS
                     WGTGKLILEIYEKTTEHGLWGPIFVTDYPEEVSPLSRAHRDQPGVTERFEAIVAGREL
                     CNAFSELVDPDVQRSRFEDQVRQKDAGDHEAMPLDEDYLRAMEYGLPPTGGLGIGIDR
                     LIMLLTGTTSIRDVVLFPTLKPEQ"
     gene            complement(6217..7194)
                     /locus_tag="VLR27_05655"
     CDS             complement(6217..7194)
                     /locus_tag="VLR27_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HSP02965.1"
                     /translation="MGSNPLLDFPGAGDHLALIDERLLAAVTTDDPQLTEMTSHLIRA
                     GGKRLRPMLGVLSAATSAEVTVDVIDGGVAVELVQVGSLYHDDVIDEADSRRGVESVN
                     VRWNNLTAILAGDFLLAKASEIAASLGTEVAALLARTIGRLCEGEIAELRHAYSTART
                     EDAYFTAIEGKTASLYATACRIGAIVGGLPRDQVDALSDYGLRFGMAFQLVDDVLDLV
                     ATEEELGKPAAHDLTEGIYNLPVLRALQSDVGDELAALLGSPIQGAEREAARSLVLQS
                     GGIEETMAEAERHARRAQDALAPLPDSPARAALHDGVDHVLGTVGAARA"
     gene            7265..8611
                     /locus_tag="VLR27_05660"
     CDS             7265..8611
                     /locus_tag="VLR27_05660"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HSP02966.1"
                     /translation="MYDWTMRLESRVDVPLPFRDRVHAGQQLGEALAGIDLGDAPVVA
                     GLPRGGVPVAAQVADRLHAPLDVIIVRKVGVPGQRELAMGAVGEGGVVVQDARVMQAV
                     APSARDVERTVTAEQAEVEARAHRFRPGRERRSLTGRTVVVVDDGLATGSTAEAAVRV
                     ARAQGADRIVVAVPVGSPAAVTRLEAVADAVLCLAAPAHFGAVGAFYDDFSETTDDEV
                     VRLLGDHARSPAARTRPAATVDVAIDVGRGRLPGTLEVPEDAAGLVLFAHGSGSSRHS
                     PRNRRVAQALRDAGLATLLFDLLLPDEEGDRRLVFDIDLLADRLLAASADVRTRAEVE
                     GLGVGVFGASTGGGAALVAAARRPDLVDAVVSRGGRPDLAGEHLGDVRAPTRLIVGGD
                     DTEVLALNRDAAAQLACDHDLVVVPGATHLFEEPGALDQAAQHTIDWFRQQLPAPR"
     gene            complement(8618..10453)
                     /locus_tag="VLR27_05665"
     CDS             complement(8618..10453)
                     /locus_tag="VLR27_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HSP02967.1"
                     /translation="MPTDLSSIPGAGGGRRRGRSGPPGRPPRQYDPPLLGDPRRSGRL
                     DAPRSRFGRRPKGPKAWTPRTQRRSIPWSSLFIALGFVLLGAYIWTQFWGATRVDVAI
                     SGLEDGAQLTLEEASGLDVRIDVAQLDLEAGLEPTLFFDGLPAVEDTYEVSEKGVRWT
                     PPALEEGVHELSMSVGRPLLDEAEFTWVFAVDGTPPMIDVASPQPAVPICSAVTVTGR
                     AETGLASITVDGEDLDLADDGTFTLEYDNAPTGPVLIQAEDVAGNRAAAEIVIPVEYP
                     SSQSMHVTAAAWGYAPLREHIIEMVDAGKVSAVQLDIKDEGGIVGYDTDVDMAHEIGA
                     VRQEYVLEDAIDLLKGKGMRIIGRIVAFRDGPMARWAVDNGRMDYVIQDSEGGLLSKY
                     GGFTNVANPDVRQYNIDLAMEAVDRGVDEILWDYVRRPEGDIAEMVFPGLQMPEGGDD
                     IAEKKAVNDAVVSFLAEAGDPLREECVYQGASLFGVAARNPDAIGQPVPAIARHVDYI
                     APMLYPSHWVRGEYRVDHPNAQPYEIINKSLTDFQTKAAGSGVAFNLWVQDFSIGIPY
                     GPAEVRAQIDAARDLGVDSWLLWNATVRYTPEAITPDIVDLPGEE"
BASE COUNT         1451 a         3930 c         3880 g         1491 t
ORIGIN      
        1 tccagcacgg tggcgtcgag ccagacgtgg tcggtccggt cggcctgcag gcgggcggtg
       61 atcgcgcggc tgaccacgtc gcggggctgg agctcgtcga cgaagcgctc acccgacgca
      121 tcgcgcagga acgcgccgtg cccccggagc gcttccgaca gcagcgggcg gggcatcgcc
      181 tcgtggtgca gcgcggtggg gtggaactgg acgaactcga cgtcggccag ggccgctccg
      241 acccgcagcg ccatggccat gccgtcgccg gtggcctcga gcgggttggt ggtgaccgag
      301 aacagctggc cgccgccgcc ggtggcgagc agcacgttgc gggcccggac ctcgatgcgc
      361 tcaccgtgct cgtcgatggc cgtgacgccg gcgcagcgac cgccctccac cagcagatcg
      421 agggcgaacg tccgctccag caggaccgca gccgtcttct ggaccgcctc gaccagcgcc
      481 cgctcgacct cgacgccggt cgccgccccg ccggcgtgga ggatccgggc gtggctgtgc
      541 ccgccctcgc gggccagcag gtactggccg cgctcgtcct ggtcgaacat ggcgccgagc
      601 gcgatgagct cgttcacccg caccggcccc tcgtcgacca ggacccgcac ggcgtcgagg
      661 tcgcagagcc cggcgccggc gccgagggtg tcggccaggt gcaggtcggt ggagtccgga
      721 tcgccaccga gcacggcggc aacgccgccc tgggcccacc gggtggtggc ctggtggagc
      781 tcggccttgg tgaggacgcc gacccgcatg ccgtgcacgt cggcggcgcg caccgcggcc
      841 gacagcccgg cgaccccgga tccgagcacg agcaggtcga gcacctccat cgccggcagc
      901 ctgccacggg acggtgcaca ccccggcggg acggccttct cgaactggca gttgtggtgt
      961 agcgcgacgt cagcagcgcc cggtccgacc gccgaggtgc tcgagcgttc gccggacctc
     1021 gtggagcacc cctcccggcg cgtacatgac ctcctcctcg tcgaagcgca gcaccttcca
     1081 cccgagccgc cgcaggacgg catcacgccg ggcatcgcgc cgccgagccg ggtcggtgcg
     1141 gtggtggggc ccgtcgatct cgacatcgag ccggaccggg atccaggcga agtcgagacg
     1201 gaacgggtcg ccgtggggtt gggggaccac gtgctggcac gtcggcagcg ggaacccggc
     1261 gtcgagcagg tcgtcgagga acacctgctc gagcgtggtg tccgggatgc cgtcgatgcc
     1321 gtggtagcgc tcgagctggc gtcgcagcgg cccgcacccg ttgcggcccc gccggctgtg
     1381 cagctcgagg atggcggcca gctgcttcca cgacacgccg tgcgaccgcc ggagctcccg
     1441 gatcacggtg gtgtacgccg tgtcgcccaa caccgcgccg atgtcgacgg cgagccggcg
     1501 cggcggcgtg caggggatgc cgtcgatgtg gatcggtggc acgtaggcga ggtctcggct
     1561 ctcgtggacg atgaccccgg gccgggtgaa cttccgcttg gacgggacgc tgagctcgat
     1621 ccaggccccc tcgtacgtgg cgctacacca gcactgccag ttcggaaagg ggccgtggca
     1681 ggctgccggc catgtcggac gtgcccgtcg aacccccgat ccatgccgtc cgcgccgcgg
     1741 tggcggctgc cctcgccgag gacctcacgc ccctcggtga cctgagtgcg gcgctggtgc
     1801 ccgccgacgc cacgatcgac gcggtgttcg ccagccgggc ggccggtgtc ctggccggca
     1861 cggcgtgcgc caccgaggcg ttcgcccagc tcgacccggc cgtgaccgtc tcgtggtccc
     1921 accacgacgg cgatcgcatc gacgccggca ccacgatcgc caccgtcacc ggcccgctcg
     1981 cccccgtgct caccggggag cgcacggcac tcaacttcct ctgccacctg tccggcatcg
     2041 ccacgctcac ccgtcgattc gccgacgccg ccgccgaggg cggcgacgcc cgcatctggg
     2101 acacccgcaa gacgacgccc ggcctgcgct cgctggagaa ggcggcggtc cgggcggggg
     2161 gtggcgccag ccaccggggg aacctgtccg actgggtcat gttcaaggac aaccacctcg
     2221 ccgccatcgg catcgaggag gcggtccggc gggcccgcac ggcgtggccg gcccgcacca
     2281 tccacgtcga ggtcgacgac ctcgacggcc tgcgcaccgc gctgaccgcc ggctgcgacg
     2341 ccgtcctgct cgacaacttc acgccggacc aggccgccga ggcggtcgcc atcgcggacg
     2401 ggtgggcggc cgagcacggt cgccgccgtc cgctgctcga gtgctcgggc ggcatcaccg
     2461 tcgacaccgc cgcggcctac gcggccgccg gcgtcgacct gctctccacc agccagatca
     2521 cccagtcggc gccggctctc gacatcgggt tggacgtccg aggctgacat ggggcgcgcc
     2581 gagggaccgt gggacctggg cggccgcacc cgacgggctg tcacccccgc tcgcctgctc
     2641 gcacgaggcg ccgccgccgc ggcgatggcg caggcacggg ccctgcgggg tgactcgatc
     2701 caggacatcg cctccgaccg ccggctggtc gccgccgccg aggacatcgc ccgcgagctg
     2761 ggggagatga agggggcggc gatgaagatc ggccaggcgc tgtcgatcct cgacgtcggc
     2821 ctcatcccgg aggagtaccg caccgccctg gccgtgctgc agtccgacgc gccgcccatg
     2881 ccctacgacg ccgtccgcga ggtcgtcacc gccgagctcg gcgctccgcc cgaggagctg
     2941 ttcgacttct tctcaccccg gcccatggcg gcggcgtcga tcgggcaggt ccacatggcc
     3001 cacctcggcg acgacgagct ggtggtcaag gtccagtacc ccggcgtcgc ggatgcggtg
     3061 gccgccgacc tgcgcaacgc cgccctcctg tcggcgctcg cccggctgat gcagcggatg
     3121 ctggccggac tcgccgggga cgtcgacgtg cgggcgctca tcgacgaggt ccgggaccgg
     3181 gtcaccgacg agctcgacta ccggatcgag gcggccaacc agctcgagtt cgcggcgcgc
     3241 ttcgccggcg acccggagat cgacatcccg gcggtgcgcg aggacctgtc cacccgacgg
     3301 gtgctcacca gcgagtacgt cgacgccatg cggtggtcgg cggcgctgga gcaaccggcc
     3361 gagctgcgca acaagtgggg caacgtcgtc tcccgcttct cggcgacctc gatctacgac
     3421 cacggggtcg tcaacgtcga cgcccacccc gggaacttcc tgttccacga ggacggccgg
     3481 gtgacgttcc tcgacttcgg ctgcgtgtcg cgcctcacgc cggaccagct cgtgcgcctg
     3541 cgccggctgg cggcagcgct catgaccggc gaccgcgacg ccgtgctcga cacgttcgtc
     3601 gatgccggct tcctgcgctc ccgggagggc ttcgacccgg aggtcctgct cggcccgctc
     3661 ggcagctggg tgcaggcggc ccggggaccc cagccgttcc gctactcccg cgagatgctg
     3721 gccgacatcg tcgccgaagc catgcgggtg cgcgtcggcg tcgacgagct caagctcctc
     3781 cagcggctcg acgtcccgcg tgagcacgtg ctgctcgggc gggtctccgt cggcaacgag
     3841 gcgatcctgg cccacctcga ggccaccatc gacttccgcg acctctacgc ccgccacctc
     3901 gccggagccc tcgacatcga tccggagtgg cccgacgcat cggatgagga cgggtccgga
     3961 ggggtcgcgt agcgtcgccc cccgtgaatc tgctcgcaat cgacgtcggc aacacgcaga
     4021 ccgtgatcgg gctctacgac ctggagtcca gcgtcgagcc gagggaccgg gtcgccgact
     4081 cggacctgct cgaccactgg cgcatcgcca ccaacgtcga gcggaccgcg gacgagtacg
     4141 ccctgctctt ccaggagttc ctggcgttcc acgggttcgg ctggaccgag gacgtgcagg
     4201 gcttcgccgt gtgctcctcc gtcccccggg tcaccggtgc gctgcgccgc ttcagcgagc
     4261 gctacctggg cttcgacgcc ctcgtcgtcg aacccggcgt gaagacgggc atgccgatcc
     4321 tctacgacaa cccccgagag gtcggctccg accgaatcgc cgacgcagtc gcggcctggg
     4381 acctctacgg cggcccgacc atcgtcgtcg acttcggcac cgccaccacc ttcgaggcca
     4441 tctcggccaa gggcgagtac atgggcggcg ccatcttccc cggcatcgac atcagcctcg
     4501 acgccctctt cggccgggcc gccgcccttc gccgggtcga gctcgtcgag cccagcaacg
     4561 tcatcggccg ctcgaccatc gagtcgatgc agtccggtgc ggtctacggc ttcaccgcgg
     4621 tcgtcgacgg catggtcgag cgcttccagg tcgagctcga ccagccctgc acggtggtgg
     4681 ccacgggcgg cctgtccggc ctcatcgccc cgctgtcgag caccatccag caccacgagc
     4741 cgtggctcac cctgcacggc ctgcggatca tccacgagaa gaacccccat gtctgacgac
     4801 gcccccttcg atccgcccta ccgcctcgac gtcaccgacc gggtcggcgc gatcctcacc
     4861 gagttcgacc acctcgccga cggcgaggag accgaccgga cggtcaccgt cgccggccgc
     4921 gtcatgctgc tccgcgacca ggggcgcctg gcgttcgcca ccctgcgcga gggcagcact
     4981 ggcgacgaga tccagctgtt cgcgctgtcc aaggtcctcg acggcttcga ccggttcgtg
     5041 aagctgaacc tgggcgactg gatcggcgcg accggcgagg tcgtgcgcac caagcggggg
     5101 gagctgtcga tcaaggtcac ctcgttcgtg ccgttggccc aggcccggcg ggggttcggc
     5161 gacaagttcc acggcatcac cgacaccgat ctgcgctacc gccagcgcta cgccgatctc
     5221 tgggccaacc ccgaggccgc cgtggccctg cgcgcccgga gccgcaccat cagcctcatc
     5281 cggcgctggc tcgaggagcg cgacttcgtc gaggtcgaga cgccggtgct gcagcacgtg
     5341 gccagcgggg cggcggccac gccgttcacg acgcaccaca acgcgctcga cgtcgacctc
     5401 aagctgcgca tcgccccgga gctgttcctc aagcgcctgg tggtcggcgg cttcgagcgg
     5461 gtgtacgaga tcggccgcgt cttccgcaac gaggggatct ccacccggca caaccccgag
     5521 ttcacgatgc tcgagctgta ccaggcctac gccgactaca cggacatgat ggcgctggtc
     5581 gaggacctcg tcgcccacct cgccacggag ctccacggca ccaccacgct cacctacgac
     5641 gaccgcgagc tggacctgtc cacgccgtgg cgccgggcgt ccatggtgga gctcatcgag
     5701 gacgccatcg gcgtgcgcct cgacgtcggc cagcccctcg acgaggtccg tgccgtcgcc
     5761 accgagcacg gcgtcgaggt cgaggggtcc tggggcaccg gcaagctcat cctcgagatc
     5821 tacgagaaga ccaccgagca cggcctctgg ggtccgatct tcgtcaccga ctaccccgag
     5881 gaggtgtcgc cgctgtcgcg ggcccaccgc gaccagcccg gcgtcaccga gcgcttcgag
     5941 gccatcgtcg ccggccgcga gctgtgcaac gcgttctccg agctcgtcga ccccgacgtg
     6001 cagcggtccc gcttcgagga ccaggtccgc cagaaggacg ccggcgacca cgaggccatg
     6061 ccgctcgacg aggactacct gcgggccatg gagtacggcc tgccgcccac cggcggcctc
     6121 ggcatcggca tcgaccggct catcatgctg ctcaccggca ccacgtccat ccgcgacgtc
     6181 gtgctgttcc cgaccctgaa gccggagcag tagggatcag gcgcgggcgg cgccgaccgt
     6241 gccgagcacg tggtcgacgc cgtcgtgcag cgcggccctg gccggtgagt ccggcagcgg
     6301 cgccagggcg tcctgggccc ggcgggcgtg gcgctcggcc tcggccatgg tctcctcgat
     6361 gccgccggac tgcagcacga gcgagcgggc cgcctcgcgc tcggcgccct ggatggggga
     6421 gccgagcagc gcggcgagct cgtcgccgac gtcggactgc agcgcccgca gcaccggcag
     6481 gttgtagatg ccctcggtga ggtcgtgcgc ggccggcttg ccgagctcct cctcggtggc
     6541 gacgaggtcg agcacgtcgt cgacgagctg gaaggccatg ccgaagcgga gcccgtagtc
     6601 ggacagcgcg tcgacctggt cgcgggggag gccgccgacg atggcaccga tgcggcacgc
     6661 cgtggcgtac agcgatgccg tcttgccctc gatggcggtg aagtaggcgt cctcggtgcg
     6721 ggccgtggag taggcgtggc gcagctcggc gatctcgccc tcgcacaggc gcccgatggt
     6781 gcgggccagc agggcggcga cctcggtgcc gagcgacgcg gcgatctcgg acgccttcgc
     6841 caggaggaag tcgccggcga ggatggcggt gaggttgttc cagcggacgt tcacggattc
     6901 gacgccccgg cgggagtcgg cctcgtcgat gacgtcgtcg tggtagagcg aaccgacctg
     6961 gacgagctcc acggccacgc cgccgtcgat gacgtcgacg gtgacctcgg cggaggtcgc
     7021 ggccgacagc acgccgagca tcgggcgcag gcgcttgccc ccggcccgga tgaggtgcga
     7081 ggtcatctcg gtgagctggg gatcgtcggt ggtgacggcg gcgaggaggc gctcgtcgat
     7141 cagcgccagg tggtccccgg cgccggggaa gtcgagcagg gggttggaac ccatggacgg
     7201 aggctactgg cgccctccga cagggccgca gtcgccatct ggggtggtca gcgcggaacc
     7261 cgtcgtgtac gactggacca tgcgcctcga gtcgcgcgtc gacgtgccgc tgcccttccg
     7321 ggatcgcgtc catgccgggc agcagctcgg cgaggcgctc gccggcatcg atctgggtga
     7381 tgcgccggtg gtcgcggggc tgccccgcgg cggggtgccg gtggcggcgc aggtcgccga
     7441 ccggctccac gcgccgctcg acgtcatcat cgtccgcaag gtcggcgtgc ccgggcagcg
     7501 cgagctggcg atgggggctg tcggcgaggg cggcgtggtg gtccaggacg cccgcgtcat
     7561 gcaggccgtc gccccctccg cgcgagacgt cgagcgcacc gtcaccgccg agcaggccga
     7621 ggtcgaggcg agggcgcacc ggttccgtcc cggtcgcgag cgtcgctcgc tgaccggacg
     7681 cacggtcgtg gtcgtcgacg acggcctcgc caccggttcc accgccgagg ccgccgtccg
     7741 tgtcgctcgc gcccaggggg cggaccggat cgtggtcgcc gtccccgtcg gctcgccggc
     7801 ggcggtgacg aggttggagg cggtggccga cgcggtgctg tgcctcgccg cgccggcgca
     7861 cttcggtgcc gtcggcgcgt tctacgacga cttctccgag acgaccgacg acgaggtcgt
     7921 gcgcctgctc ggagaccacg cccggtcgcc cgcagcccgc acgcggccgg cggcgaccgt
     7981 ggacgtggcg atcgacgtcg gacgcggccg gctccccggc acgctcgagg tccccgagga
     8041 cgccgccggt ctcgtgctgt tcgcccacgg cagcggcagc agccggcaca gcccgcggaa
     8101 ccggcgggtc gcccaggcgc tgcgcgacgc cgggctcgcc accctgctgt tcgacctgct
     8161 gctccccgac gaggagggcg accgccgcct cgtgttcgac atcgacctgc tggccgatcg
     8221 cctgctggct gcgtccgccg acgtgcgcac ccgggccgag gtcgagggcc tcggggtcgg
     8281 ggtcttcggc gccagcaccg gcggtggcgc agcactcgtc gccgcagcgc gacgcccgga
     8341 cctcgtcgac gcggtggtgt cgcggggcgg acggcccgac ctcgcaggcg agcacctcgg
     8401 cgacgtgcgt gctcccaccc gcctgatcgt gggaggcgac gacaccgagg tgctggccct
     8461 caaccgggac gcggcagccc agctggcctg cgaccacgac ctcgtcgtgg tccccggtgc
     8521 cacccacctg ttcgaggagc cgggtgcgct cgaccaggcg gcccagcaca ccatcgactg
     8581 gttccgccag cagctgccgg cgccgcgctg agcccggcta ctcctcgccg ggcaggtcga
     8641 cgatgtcggg ggtgatcgcc tccggcgtgt agcgaaccgt cgcgttccac agcagccagc
     8701 tgtcgacgcc caggtcgcgg gccgcgtcga tctgggcccg cacctcggcc ggcccgtacg
     8761 ggatgccgat ggagaagtcc tggacccaca ggttgaacgc cacgcccgag cccgccgcct
     8821 tggtctggaa gtcggtgagc gacttgttga tgatctcgta gggctgggcg ttggggtggt
     8881 cgacccggta ctcgccgcgc acccagtgcg acgggtagag catcggtgcg atgtagtcga
     8941 cgtggcgggc gatggcgggc accggctggc cgatggcgtc ggggttgcgg gcggcgacgc
     9001 cgaagaggga ggcgccctgg tacacgcact cctcgcgcag ggggtcaccc gcttcggcga
     9061 ggaacgacac gacggcgtcg ttcacggcct tcttctccgc gatgtcgtcg ccgccctcgg
     9121 gcatctgcag gccggggaag accatctcgg cgatgtcgcc ctcgggccgg cggacgtagt
     9181 cccacaggat ctcgtcgacg ccgcggtcga ccgcctccat ggcgaggtcg atgttgtact
     9241 ggcgcacgtc ggggttggcg acgttcgtga agccgccgta cttggagagc agcccgcctt
     9301 cgctgtcctg gatcacgtag tccatgcggc cgttgtcgac ggcccagcgg gccatgggcc
     9361 cgtcgcggaa ggccacgatc cggccgatga tgcgcatgcc cttgcccttg agcaggtcga
     9421 tggcgtcctc gagcacgtac tcctggcgga cggcgccgat ctcgtgggcc atgtcgacgt
     9481 cggtgtcgta gccgacgatg ccgccctcgt ccttgatgtc gagctgcacg gcggagacct
     9541 tgcccgcgtc gaccatctcg atgatgtgct cccgcagcgg ggcgtagccc caggcggccg
     9601 ccgtgacgtg catggactgc gacgacgggt actcgacggg gatgacgatc tcggcggcgg
     9661 cccggttccc ggcgacgtcc tcggcctgga tgaggacggg cccggtcggc gcgttgtcgt
     9721 actcgagcgt gaaggtcccg tcgtcggcga ggtcgaggtc ctcgccgtcc acggtgatgc
     9781 tggccaggcc ggtctcggcc cggcccgtga cggtgacggc cgagcagatg gggaccgcgg
     9841 gctggggcga ggcgacgtcg atcatcggcg gggtgccgtc gacggcgaag acccacgtga
     9901 actcggcttc gtcgagcagg ggccgaccga ccgacatgga cagctcgtgc accccctcct
     9961 cgagcgccgg cggggtccag cgcacgccct tctcggagac ctcgtaggtg tcctccacgg
    10021 ccggcaaccc gtcgaagaac agcgtcggct ccagccccgc ctcgaggtcg agctgggcga
    10081 cgtcgatgcg cacgtcgagg ccgctggcct cctcgagggt gagctgggcg ccgtcctcca
    10141 ggccgctgat ggcgacgtcg acccgggtcg cgccccagaa ctgggtccag atgtaggcgc
    10201 cgagcagcac gaacccgagg gcgatgaaca ggctcgacca ggggatcgac cggcgctggg
    10261 tgcggggcgt ccaggccttg gggcccttcg gtcgccgtcc gaagcgggag cgcggggcat
    10321 cgaggcggcc gctgcgacgc gggtcgccga gcagcggtgg gtcgtactgg cgaggagggc
    10381 gtcccggtgg accggagcgg ccgcggcggc ggccgccacc tgcgcccggg atgctgctga
    10441 gatcggtggg catggtggtg ggaggccgga ctcggcgccg tggcgggccg tccggcgcga
    10501 ccgacaacgc taccggggcc tccgtccggc ccgccatgcg ccccgccccg gtggagcgct
    10561 ccggggcagg gatacactgg cggcccgagt ctcccgatca gtcctggtcg taccccaggg
    10621 agggcagcgc cttgttcgaa cgtttcaccg accgtgcccg ccgagtcgtc gtgctcgcgc
    10681 aggaagaggc acggctcctc aaccacaact acatcggcac cgagcacatc ctgctcgggc
    10741 tgatccacga gg
//
LOCUS       DATISU010000136         2640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_192767, whole genome shotgun sequence.
ACCESSION   DATISU010000136 DATISU010000000
VERSION     DATISU010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192767"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VLR27_05670"
     CDS             <1..948
                     /locus_tag="VLR27_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HSP02968.1"
                     /translation="GGTQNVLVRFTRSGRGHVLRRPPLHKRANSDETMRREARVLAAL
                     AGSDVPHPGLIAACPDTEVLGAAFYLMEPIDGFNPASGLPEPHRSDPAMRRAMGLSMV
                     DAIAALALVDHEAVGLGDFGKPEGWLERQVGRWRSQLDSYAELDGYPGPDIPGVDEVS
                     AWLEHNRPATWQPGIIHGDFHFANVLVRPDAPDLAAIVDWELCTIGDPLLDLGHLLAT
                     WPPAGEATEGAGAVGAGISDLPTKPELVARYAERTGRPTTDVDWYHVLAAFRLGIILE
                     GTHARAFAGKAPKEIGDLLHATTVGLFERALAVIGTAGV"
     gene            1076..1618
                     /locus_tag="VLR27_05675"
     CDS             1076..1618
                     /locus_tag="VLR27_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP02969.1"
                     /translation="MAVLDAVLELFEEGNLDPGVHEVADRSGVSLRSVYRYFEDLDEL
                     ITAAIERHLERTRPLFDIERVGEGPLPERINRFADRRLRLFDAMRVIYRASRVRAATD
                     PQVQAGLMDTYQWLGEQAHQMFAPELEQIDDPAERLEARHTVDLLTQFDSLEHLRHRC
                     GLDRDETVGYLRRSLNRVLA"
     gene            1680..>2640
                     /locus_tag="VLR27_05680"
     CDS             1680..>2640
                     /locus_tag="VLR27_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HSP02970.1"
                     /translation="MRSPKDFFAPLAIGAPAPLREVPFRPSRNIHFFPPANEKMRSKV
                     PDMVATCDVLLGNLEDAVPASDKEAARAGLIEVGQSVDFGDTQFWTRVNSLDSPWGLD
                     DLLEIVPAIGDKLDVIMIPKVEGPEDIHYVDRLLAQLEAKAGLTEPLLIHAILETARG
                     VTNVEEICAASPRMQGLSLGPADLAANRRMKTTRVGGGHPGYLVRTDPTGDDLEAGRT
                     TYQQDLWHYTVARMVDACAANGIYAYYGPFGDIRDTVGCEDQFRNAYLLGCVGAWSLH
                     PVQIDIAKKVFSPPVEDVVHAKRVVEAMGDGTGAVMLDGKMEDD"
BASE COUNT          402 a         1017 c          863 g          358 t
ORIGIN      
        1 ggcggcaccc agaacgtgct ggtccgcttc acccgcagcg gtcggggcca cgtcctgcgg
       61 cgaccaccgc tgcacaagcg agcgaactcc gacgagacga tgcgccgcga ggcccgcgtc
      121 ctggctgccc tggccggctc cgacgtcccg cacccgggcc tgatcgccgc atgccccgac
      181 accgaggtgc tgggcgccgc cttctacctg atggagccga tcgacggctt caacccggcc
      241 agcgggctcc ccgaaccgca ccggtcggat ccggcgatgc gacgggcgat gggcctgtcg
      301 atggtggacg ccatcgcggc tctggcgctc gtcgaccacg aggcggtcgg gctcggtgac
      361 ttcggcaagc ccgaggggtg gctcgagcgc caggtcggcc ggtggcgcag ccagctggac
      421 tcctacgccg agctcgatgg gtacccgggc cccgacatcc ccggcgtcga cgaggtgtcc
      481 gcctggctgg agcacaaccg cccggccacc tggcagcccg ggatcatcca cggggacttc
      541 cacttcgcca acgtcctcgt ccgacccgac gcccccgacc tcgccgccat cgtcgactgg
      601 gagctgtgca ccatcggcga cccgctgctc gacctcggcc acctgctggc cacgtggccg
      661 cccgccggcg aggcgacgga aggcgccggc gcggtcgggg ccgggatcag cgacctcccc
      721 accaagcccg agctggtggc ccggtacgcg gagcggaccg gacggcccac caccgacgtg
      781 gactggtacc acgtgctggc ggccttccgg ctcgggatca tcctcgaggg aacccacgcc
      841 cgggccttcg ccggcaaggc acccaaggag atcggggacc tgctgcacgc caccacggtc
      901 gggctcttcg agcgcgcgct cgccgtcatc ggcacggcgg gtgtctgaac cgcgcgccgc
      961 acggataggg agcactcgtc ggcgggcccg atacgagtgg atggcgcaca tggaccagac
     1021 gactgaagca gagagccgtg tggacgggcg gacggcacgg cgagatcgaa accgcatggc
     1081 ggtgctcgat gccgtgctcg agctgttcga ggagggcaac ctggaccccg gcgtccacga
     1141 ggtggccgac cggtccggcg tgtcgctgcg gtcggtgtac cgctacttcg aggacctcga
     1201 cgagctcatc accgccgcca tcgagcgcca cctcgagcgc acccgcccgc tgttcgacat
     1261 cgaacgggtc ggcgagggtc ccctgccgga gcggatcaac cggttcgccg atcgccggct
     1321 gcggctcttc gacgccatgc gcgtcatcta ccgagcgtct cgtgtccggg ccgccaccga
     1381 cccccaggtg caggccggcc tgatggacac ctaccagtgg ctcggcgagc aggcgcacca
     1441 gatgttcgcc cccgagctgg agcagatcga cgaccccgcc gagcgcctcg aggctcgcca
     1501 caccgtcgac ctgctgaccc agttcgactc cctcgagcac ctccgccacc gctgcggcct
     1561 cgaccgcgac gagacggtcg gctacctgcg ccgcagcctc aaccgcgtcc tcgcctgagg
     1621 tttctcgggg ttccctgagg tttcccgagg ttcggtgacc ggcggtacgg tctggcccga
     1681 tgcgcagccc caaagacttc ttcgctcccc tggccatcgg cgccccggca ccgctgcgtg
     1741 aggtcccgtt ccggccctcg cgcaacatcc acttcttccc gccggcgaac gagaagatgc
     1801 ggtccaaggt gcccgacatg gtcgccacct gcgacgtgtt gctcggcaac ctcgaagacg
     1861 ccgtgcccgc ctccgacaag gaggcggccc gcgccggcct gatcgaggtc ggccagtccg
     1921 tcgacttcgg cgacacccag ttctggaccc gggtcaacag cctcgactcc ccctggggtc
     1981 tcgacgacct gctcgagatc gtcccggcca tcggcgacaa gctcgacgtg atcatgatcc
     2041 ccaaggtcga gggccccgag gacatccact acgtcgaccg cctcctcgcc cagctcgagg
     2101 ccaaggccgg actcaccgag ccgctcctca tccacgccat cctcgagacg gcccggggcg
     2161 tcaccaacgt cgaggagatc tgcgccgcct cgccccgcat gcagggcctc tccctcggcc
     2221 cggccgacct ggccgccaac cgccgcatga agaccacccg cgtcggcggc ggacaccccg
     2281 ggtacctggt gcgcaccgac cccaccggcg acgacctcga agccgggcgc accacctacc
     2341 agcaggacct gtggcactac acggtcgccc gcatggtcga cgcctgcgcc gccaacggca
     2401 tctacgccta ctacggcccc ttcggcgaca tccgcgacac cgtcggctgc gaggaccagt
     2461 tccgcaacgc ctacctgctc ggctgcgtcg gcgcctggtc gctgcacccg gtgcagatcg
     2521 acatcgccaa gaaggtgttc tccccgcccg tcgaggacgt cgtgcacgcc aagcgggtgg
     2581 tcgaggccat gggcgacggc accggcgccg tgatgctcga cgggaagatg gaggacgacg
//
LOCUS       DATISU010000137        12090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_192835, whole genome shotgun sequence.
ACCESSION   DATISU010000137 DATISU010000000
VERSION     DATISU010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 12090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12090
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192835"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..3488
                     /gene="mfd"
                     /locus_tag="VLR27_05685"
     CDS             57..3488
                     /gene="mfd"
                     /locus_tag="VLR27_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HSP02971.1"
                     /translation="MTAAVSLAPTDDLRHLPTVARDEPGLLAVLGRKDAVLAVPEPAR
                     ALVVAGLAARSTRRPIVVATAATADAERLVNDLQALLGPDAVEHFPAWETLPFERVSP
                     AIETMGRRLKTLHRLGAGRDDPDQLPDVVVTSVRALIQRLAPGVEDIEPVCITKGDEV
                     DIEGLVTSLVEFGYRREYQVEHRGEVAVRGSIVDVFPATADRPVRIDLWGDEVDRLTE
                     FSVTDQRTTDDVDAVEILPCRELLPSAAVRERAEGLIAREPWGREQWERIAQGQLFDG
                     MESWLPWLAPEERVLADLLPEDAQILLVEPRRMRDRAADFLAEEADLAQTLAKTWGAV
                     SDGEAHDFPPLHLPFERVLSHATAATWHLTTSPEGPDTPTVQAMTFQVASGDEDRRRA
                     QLRDLRADGYRIVIAADGEGSAARLRELVTEAGVEAEIVVVPLERGCILPGVRLALLA
                     ESDLTGRRRSHRKAKRRRRDAQKFFDDLQAGDHVVHHQHGVARYGGMVKRAIGGVERD
                     YLLLEYRDGDKLYVPSDQIDAVRHYTGGDAPSLTRLGGDAWSKTKARVRSVVNEIAQE
                     LVVLYQKRLAAGGHAFAGDTPWQQQFEDAFPYEPTPDQLRAVEEIKGDMEIEAPMDRL
                     VVGDVGFGKTEVALRAVFKAVQDGKQVAVLVPTTLLAQQHFQTFSERFADYPVRVEVL
                     SRFLTAGQAKAVMEGCRTGAVDIVVGTHRLLNKDVSFHDLGLLVVDEEQRFGVKHKER
                     IKEFKHDVDVLTLTATPIPRTLEMSLTGIRDMSLLETPPADRQPILTYVGEFDERAVA
                     EAIRRELLREGQVFYVHNRVRDIDQKAEELREIVPEARIAVAHGQMDEGTLEQVVIDF
                     WEGRFDVLVCTTIIESGIDMPTVNTLVVERADLLGLGQLHQIRGRVGRSGSRAYAYLF
                     HPQDMVLSEEAYERLRTIGESTELGSGFKIAMRDLEIRGAGNLLGTGQSGHIAAVGYD
                     LYCQMVTEAVAELKGEPVREPAEVKIETLEPAHLPPDYVEREELRLEAYRRLAAVTSD
                     AEVDDIAREWEDRYGPIPDPAKALLSVARLRAECHRVGVTEVTVVTGDGFMGPAYKAR
                     LSPVPLKASQQTRLKRIAPKAIHKPDSGLLEVPMAKGSDPTADLVTFLRHLLPPE"
     gene            3591..4262
                     /locus_tag="VLR27_05690"
     CDS             3591..4262
                     /locus_tag="VLR27_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02972.1"
                     /translation="MRTTRRPRLLPALALLVLSLVAGACAGDGELGGPTLGGTPAATV
                     GDTTVSSSDLSDEVEQWAANPAMLQAIGVPEIGTEGRRSAALVSFVLSHRIVSEQART
                     MLADARDLAADNEVDLAEAGVSGEALADPDDGEIDGILAQLDQQFTSPDGQQIFLEYP
                     EDFRRRLATDLAFQERLPAPLQLGVEAPEVSVNPKFGTTEVLQGGIVQVVGPVGPRPA
                     PLTGV"
     gene            4266..5600
                     /locus_tag="VLR27_05695"
     CDS             4266..5600
                     /locus_tag="VLR27_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HSP02973.1"
                     /translation="MGPAPRSARPRVAVVGLGPAGPDLTTVGATARLAAAPARFLRTH
                     RHPAAEPWADDPSFDHHYESAATFDEVYAAIVEDLVAAATRHGEVAYAVPGSPHVLER
                     AVAALLDDDRVEVDLVPGMSFLDLAWAHLGVDPVEAGVRLVDGHRFAIDAAGATGPLL
                     VAHCHANHVLSDLKLAFEDDEPESAVVLQRLGLPDQHVVEVAWADLDRAVEADHLTSV
                     FIPSVTTPVAAELVRFAELVRTLREQCPWDREQTHASLTRYVVEETYEVVEAIEAYDP
                     ETGDGVEDLEEELGDLLFQVVFHATIAAESGAFTLADVARGIHDKLHRRHPHVFGDAA
                     DDPGSITRTWEEIKREEKGRGSVMEGIPKALPSLLYAHKVLSKAERIGATVPAPADPA
                     AVDPDGELGAALLALVAHARAQGIDAEAALRTATDRLRDAAIRHEAEAAGEG"
     gene            complement(5604..8048)
                     /locus_tag="VLR27_05700"
     CDS             complement(5604..8048)
                     /locus_tag="VLR27_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02974.1"
                     /translation="MTTADSLLDELDAVAGSFALHLRTRDVARGVDVLRRVAEAVRRT
                     IRDGVVPPRTSPVAPLPDGPIVRLDDLPTDPLTRRRVADIVDELVAEAGFEHGAAVLC
                     EVDGLADLDECPRAVVLRLFPSPQGSAGHLPASWIDLAGEWVFGDQDPETMVRLRVLG
                     VEATVMAKEAAGILHGCARARAWCDVVTGDVTTRVRTASISFGVAPHVAVAAGGPRCD
                     DAGLLARFELLADVARDHAAPATRGRDEDRVAYACLDFEDTFGDLGTGLSGAPWAEDS
                     GASPNRVAGRLVDRRVPEVFAFQVLGPGHVATLGEAGRDLRPIGEDRWELALGDPADW
                     LPATALRDELRREGWQLLAPLLVRDDELDDELAGAGTEAEPDVPASDGAWTTGLPDLS
                     TIVLEPHSHPRRGSRLSVLELAAWLAGELHSDDPANVSPVLRVYARELAAGIDDPLRQ
                     TLRSRASRLAGTGAFDDATERRRSWVLADRLLRLHASRWLGAAGLSESAERLRNLESI
                     ATDTQLVRGVHLLGNAIGTASRRLETTRAIAGDRAELVDALAWEIWEEVSERSGGAAT
                     AEAVVHGVPGPLTFATDQRVMELSRDPDHRVELEASTRGLGDEVWSTALAEVAATAWR
                     TGWAAAETFVHHESTFSVRTTLRRTLQELLGDDDEIALDMLLDDIDATARDTIARLLL
                     VGDDQSDYWDKAMSACADVDGGRDWLRAVEETRAVLGPSLCDEAIQTARDQVGEWAAR
                     APELVSRAVVASVTREACSTAGRGVIARVAAEALAKGASQADAEDASRDAVASLTDSL
                     AEDAVAVLDELVELDA"
     gene            8180..9469
                     /gene="eno"
                     /locus_tag="VLR27_05705"
     CDS             8180..9469
                     /gene="eno"
                     /locus_tag="VLR27_05705"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HSP02975.1"
                     /translation="MTSIEHVEGREVLDSRGNPTVEVEVELESGATGRAIVPSGASTG
                     QFEAVELRDGGDRFGGKGVLDAVDNVNGDIADVVLGLEAADQRGVDQAMLDLDGTENK
                     ARLGANAILGVSLAVARAAAEERGLPLFRYVGGANAHVLPAPMMNVLNGGAHADNNVD
                     FQEFMVMPLGAASFSEALRWGVETYHALKGILHDRGLSTAVGDEGGFAPDLGSNEEAV
                     QLLVDAIEKTGRTPGQDIAIALDPANSEIFRDGEYHLEGEGRTLSGEGLAEYLSSWVD
                     KYPIVSIEDGLDEEDWDGWAALTASIGDRCQLVGDDLFVTNSTRLARGIESGVANSIL
                     VKVNQIGSLTETLDTVGLAIRSGYTAVMSHRSGETEDTTIADLAVATNCGQIKTGAPA
                     RSDRVAKYNQLLRIESLLGDTARYRGWDALSPGVQPR"
     gene            9466..9906
                     /locus_tag="VLR27_05710"
     CDS             9466..9906
                     /locus_tag="VLR27_05710"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HSP02976.1"
                     /translation="MTAATPAPRAGLRRPHLPSGIGRWIRPLLLATAAVVTIAALAVY
                     VFPTRTWLDQRAALAETSVELRELEAERSALEQRVAELDSDGQIEEIARSQYGLVRPG
                     EEAYAVLPAPEKPVELPALWPFGDLLEPTTGEPTAPTGADAGRD"
     gene            9958..>12090
                     /locus_tag="VLR27_05715"
     CDS             9958..>12090
                     /locus_tag="VLR27_05715"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSP02977.1"
                     /translation="MSILGNRVLRSEDPKFLTVGGSYVADLRDPLLEGALHVAYVRSM
                     AAHGTITGVDVEEARNAPGVVAVFTGEDLGDLPDMPLAVPLLNPQHAQPVLAKGKVRF
                     VGEAIAAIVAETPTAAADAAELVWADIDFLPAVLTPEDALAGDTLLFEESGTNAAIEL
                     LFGEDDAAFEGCEVVVEHDFLNQRVAGCPLEVRSAAATWDGERLVHFGSTQAPHGVKA
                     TLQAVYGLHESGVRVVAPDVGGGFGPKIGAYPEEVLLGWLSKQVGRPVRWTETRSENM
                     VAMGHGRGQWQQIKIGGTRDGKVTAYRLNLTQDAGAYAALGAFLPFLTRVMACGVYDI
                     PAVQFNARSVLTNTTPVVAYRGAGRPEATAAIERAMDLFAAEIDMDPAEVRRRNLIAA
                     DDFPHTTATDAVYDVGDYERSLDLALDAAGYDDLRAEQARRRDAGDPVQLGIGVSVYV
                     EVTAGPQAGEEFGRVVVHPDGSATAFTGSSAHGQGHDTSFAMLVTDTLGIPLEQVTVK
                     HGDTDEVARGVGTFGSRSLQLGGSAIFQASGQVLDKARELAAELLEADPADIELDRAA
                     GAFHVQGTPAVSRSWADVASAAGESGLAAETDFKADSPTFPFGSHVAVVEVDTETGMV
                     RLQRMITCDDAGRILNPMLVEGQRHGGIAQGVAQALYEWVRYDDDGNPQTANLADYTM
                     ISAAELPSFELVSMETATPLNPLGAKGIG"
BASE COUNT         1672 a         4707 c         4169 g         1542 t
ORIGIN      
        1 cggtcgatcc gaacgcaagg aacgagttcg gggtggtgct gggtagcctc ggttcggtga
       61 ccgctgcagt ctccctcgcc cccacggacg acctgcggca cctccccacc gtcgcccggg
      121 acgagcccgg tctactggcc gtgctcggcc gcaaggacgc ggtgctggcg gtgcccgagc
      181 ctgcgcgcgc cctggtcgtc gccggcctgg cggcccgctc gacccgccgc ccgatcgtcg
      241 tcgccaccgc cgccaccgcc gacgccgagc gcctggtgaa cgacctgcag gcgctgctcg
      301 ggcccgacgc cgtcgagcac ttcccggcgt gggagaccct gcccttcgag cgggtcagcc
      361 ccgccatcga gacgatgggc cggcgcctca agaccctgca ccgactcggc gccggccggg
      421 acgaccccga ccagctgccc gacgtggtcg tcacctccgt gcgggcgctc atccagcgcc
      481 tcgcccccgg cgtcgaggac atcgagccgg tgtgcatcac caagggcgac gaggtcgaca
      541 tcgagggcct cgtcaccagc ctcgtcgagt tcggctaccg gcgcgagtac caggtcgagc
      601 accgcggcga ggtcgccgtg cgcggctcga tcgtcgacgt cttccccgcc accgccgacc
      661 gtcccgtgcg catcgacctg tggggcgacg aggtcgaccg cctcaccgag ttctcggtca
      721 ccgaccagcg caccaccgac gacgtcgacg ccgtcgagat cctgccctgc cgcgagctgc
      781 tgcccagcgc cgcggtgcgg gagcgggccg agggcttgat cgcccgcgag ccgtggggcc
      841 gggagcagtg ggagcgcatc gcccagggcc agctcttcga cggcatggag tcctggctgc
      901 cgtggctggc gccggaggag cgggtgctcg ccgacctgct gcccgaggac gcccagatcc
      961 tgctggtcga gccccgccgc atgcgcgacc gcgccgccga cttcctcgcc gaggaggccg
     1021 acctcgccca gacactggcc aagacctggg gtgcggtgtc cgacggcgag gcccacgact
     1081 tcccgccgct gcacctgccc ttcgagcggg tgctgtcgca cgccaccgcc gccacctggc
     1141 acctgaccac ctcgcccgag ggtccggaca ccccgaccgt gcaggcgatg accttccagg
     1201 tcgcgtccgg tgacgaggac cgccgccggg cccagctgcg cgacctgcgc gccgacggct
     1261 accgcatcgt catcgccgcc gacggcgagg ggtcggccgc ccgcctccgg gagctggtca
     1321 ccgaggccgg cgtcgaggcc gagatcgtgg tcgtcccgct ggaacggggc tgcatcctgc
     1381 cgggcgtgcg cctcgcgctc ctggccgagt ccgacctcac tggccgacgc cgcagccacc
     1441 gcaaggccaa gcgccgccgt cgcgacgccc agaagttctt cgacgacctc caggccggcg
     1501 accacgtcgt gcaccaccag cacggcgtcg cccgctacgg cggcatggtc aagcgggcca
     1561 tcggcggcgt cgagcgcgac tacctgctgc tcgagtaccg cgacggcgac aagctctacg
     1621 tgccgtcgga ccagatcgac gccgtccgcc actacaccgg cggcgacgcg ccgtcgctca
     1681 cccgcctcgg tggtgacgcg tggtccaaga ccaaggcccg cgtccgctcg gtggtcaacg
     1741 agatcgccca ggagctggtg gtcctctacc agaagcgcct ggcggcgggc ggccacgcgt
     1801 tcgccggcga caccccgtgg cagcagcagt tcgaggacgc cttcccctac gagcccaccc
     1861 ccgaccagct ccgcgccgtc gaggagatca agggcgacat ggagatcgag gcgccgatgg
     1921 accggctcgt cgtcggcgac gtcggcttcg gcaagaccga ggtggcgctg cgcgccgtgt
     1981 tcaaagcggt ccaggacggc aagcaggtcg ccgtgctcgt gcccaccacg ctgctcgccc
     2041 agcagcactt ccagacgttc tccgagcgct tcgccgacta cccggtgcgg gtcgaggtgc
     2101 tgtcgcgctt cctcaccgcc gggcaggcca aggccgtgat ggagggctgc cgcaccggcg
     2161 cggtcgacat cgtggtcggc acgcaccgcc tgctgaacaa ggacgtctcc ttccacgacc
     2221 tcggcctgct cgtggtcgac gaggagcagc gcttcggcgt gaagcacaag gagcggatca
     2281 aggagttcaa gcacgacgtc gacgtgctca ccctcaccgc cacgcccatc ccgcgcacgc
     2341 tggagatgag cctcaccggc atccgcgaca tgagcctgct ggagacgccc ccggccgacc
     2401 gccagccgat cctcacctac gtcggcgagt tcgacgagcg ggccgtggcc gaggccatcc
     2461 gccgcgagct cctccgcgaa ggccaggtct tctacgtgca caaccgggtg cgcgacatcg
     2521 accagaaggc cgaggagctg cgggagatcg tgccggaggc gcgcatcgcg gtggcgcacg
     2581 gccagatgga cgagggcacc ctcgagcagg tggtcatcga cttctgggag ggccgcttcg
     2641 acgtcctggt ctgcacgacg atcatcgagt cgggcatcga catgccgacg gtgaacacgc
     2701 tggtggtcga gcgggccgac ctgctcggcc tcggtcagct ccaccagatc cgtggtcgcg
     2761 tcggtcgctc cggcagccgg gcctacgcct acctcttcca cccccaggac atggtgctgt
     2821 cggaggaggc ctacgagcgg ctccgcacca tcggcgagtc caccgagctg ggctcgggct
     2881 tcaagatcgc catgcgcgac ctcgagatcc gtggcgccgg caacctgctc ggcaccggcc
     2941 agtccggcca catcgccgcg gtcggctacg acctctactg ccagatggtc accgaagcgg
     3001 tcgccgagct gaagggcgaa ccggtgcgcg agccggccga ggtcaagatc gagaccctcg
     3061 agcccgccca cctcccgccc gactacgtcg agcgcgagga gctgcgcctc gaggcctacc
     3121 gccgcctggc cgcggtcacg agcgacgccg aggtggacga catcgcccgg gagtgggagg
     3181 accgctacgg cccgatcccc gacccggcca aggccctgct gtcggtcgcc cggctgcgcg
     3241 ccgagtgcca ccgggtgggc gtcaccgagg tcaccgtcgt caccggcgac gggttcatgg
     3301 gcccggccta caaggcccgc ctcagcccgg tgccgctcaa ggccagccag cagacccgcc
     3361 tgaagcgcat cgcccccaag gcgatccaca agcccgacag cgggctgctc gaggtcccga
     3421 tggccaaggg ctcggatccg accgccgacc tggtcacctt cctgcgccac ctcctcccac
     3481 ccgagtagcc cactcgttct ggcctacgga tgtgccacct ggtggtcatc gatgtatgcc
     3541 agaacggacg gttcacctgg agcccggcgc ggctcctagc atcggcgacc gtgcgcacca
     3601 cccgccgccc ccgcctcctg cccgctctgg ccctgctcgt gctgagcctg gtcgccggcg
     3661 cctgcgccgg tgacggcgag ctcggcggac ccaccctcgg cggcaccccg gcggccacgg
     3721 tcggcgacac caccgtctcg agcagcgacc tgtccgacga ggtcgagcag tgggcggcca
     3781 acccggccat gctccaggcc atcggtgttc ccgagatcgg caccgaaggc cgtcgctcgg
     3841 ccgcgctggt ctccttcgtg ctctcccacc gcatcgtgtc cgagcaggcc cgcaccatgc
     3901 tggccgacgc ccgcgacctg gcggccgaca acgaggtcga cctcgccgag gccggcgtca
     3961 gcggtgaggc gctcgccgac cccgacgacg gcgagatcga cggcatcctc gcccagctcg
     4021 accagcagtt caccagcccc gacggccagc agatcttcct cgagtacccg gaggacttcc
     4081 gccggcgcct ggccaccgac ctcgccttcc aggagcgcct gccggccccg ctccagctcg
     4141 gggtcgaggc ccccgaggtg tcggtgaacc ccaagttcgg caccaccgag gtcctgcagg
     4201 gcggcatcgt ccaggtcgtc ggcccggtcg gcccccgccc ggccccgctc accggggtct
     4261 gagccgtggg cccggccccc cggtcggccc ggccacgcgt cgcggtggtc ggcctcggcc
     4321 cggccggacc cgacctcacc accgtcggcg ccaccgctcg cctcgcggcc gcgccggccc
     4381 ggttcctgcg cacgcaccgc cacccggcgg ccgagccgtg ggcggacgat ccctccttcg
     4441 accaccacta cgaatcggcc gccaccttcg acgaggtcta tgcggcgatc gtggaggacc
     4501 tggtcgccgc ggccacccgg cacggcgagg tcgcctacgc cgttcccggc tcgccccacg
     4561 tcctcgagcg cgccgtcgcc gccctgctcg atgacgaccg ggtggaggtc gacctcgtgc
     4621 cgggcatgtc gttcctcgac ctggcctggg cgcacctcgg cgtcgatccg gtcgaagcag
     4681 gtgtccggct ggtcgacgga catcgcttcg ccatcgacgc ggcgggcgcg accggtccgc
     4741 tcctggtcgc gcactgccac gccaaccacg tgctgtccga cctgaagctg gccttcgagg
     4801 acgacgagcc cgagtcggcc gtcgtcctgc agcgcctcgg cctccccgac cagcacgtgg
     4861 tcgaggtggc ctgggccgac ctcgaccgtg ccgtcgaggc cgaccacctc acctccgtct
     4921 tcatcccgtc ggtgaccacg ccggtcgcag ccgagctggt gcgcttcgcc gagctggtgc
     4981 gcaccctccg ggagcagtgt ccgtgggatc gggagcagac ccatgccagc ctcacccgct
     5041 acgtggtgga ggagacctac gaggtcgtcg aggccatcga ggcctacgac ccggagaccg
     5101 gcgacggggt ggaggacctc gaggaggagc tgggcgacct gctcttccag gtcgtgttcc
     5161 acgccaccat cgccgccgag tccggggcgt tcaccctggc ggacgtggcc cgcggcatcc
     5221 acgacaagct ccaccgccgc cacccccacg tgttcggcga cgccgccgac gatcccggct
     5281 cgatcacgcg gacgtgggag gagatcaagc gggaggagaa ggggcggggt tcggtgatgg
     5341 agggcatccc caaggccctg ccgtcgctgc tctacgccca caaggtgctg tcgaaggccg
     5401 agcgcatcgg tgcgaccgtg cccgccccgg ccgaccccgc ggcggtcgac cccgacggcg
     5461 agctcggcgc cgcgctgctg gcgctggtgg cccacgcccg agcgcagggg atcgacgccg
     5521 aggcggcgct gcgcaccgcc accgaccgcc tccgcgacgc cgccatccgc cacgaggccg
     5581 aggcggccgg cgagggctga cgcctacgcg tcgagctcga ccagctcgtc gagcacggcg
     5641 acggcgtctt cggcgagcga gtcggtgagc gacgccaccg cgtcgcggga cgcgtcctcg
     5701 gcgtcggcct ggctcgctcc cttggcgagg gcctcggccg ccacccgggc gatcacgccg
     5761 cgaccggcgg tgctgcacgc ctcgcgggtc accgacgcaa ccaccgctcg gctcaccagc
     5821 tcgggtgccc gggcggccca ctcgccgacc tggtcccgtg ccgtctggat cgcctcgtcg
     5881 cacagcgacg gccccagcac ggcgcgggtc tcctccacgg ctcgcagcca gtcccggcca
     5941 ccgtcgacgt cggcgcacgc cgacatcgcc ttgtcccagt agtcgctctg gtcgtcgccc
     6001 accagcagca gccgggcgat ggtgtcgcgc gccgtcgcgt cgatgtcgtc gaggagcatg
     6061 tcgagggcga tctcgtcgtc gtcgcccagc agctcctgca gggtgcggcg cagcgtcgtc
     6121 cgcaccgaga acgtggactc gtggtgcacg aaggtctcgg cggcggccca gccggtgcgc
     6181 cacgccgtcg ccgcgacctc cgccagcgcg gtggaccaca cctcgtcgcc gagaccccgg
     6241 gtcgacgcct cgagctcgac gcggtgatcc ggatcgcggg acagctccat cacccgctgg
     6301 tcggtggcga acgtgagcgg accgggcacg ccgtggacca cggcctcggc ggtggcggcg
     6361 ccgccggagc gctcgctcac ctcctcccag atctcccagg cgagggcgtc cacgagctcg
     6421 gcccggtcgc cggcgatggc gcgggtggtc tcgagccggc ggctggcggt gccgatggcg
     6481 ttgccgagca ggtgcacgcc ccgcacgagc tgggtgtcgg tggcgatcga ctcgaggttg
     6541 cggaggcgtt cggcggactc gctcagcccg gccgcgccga gccaccgcga cgcgtggagc
     6601 cgcagcagcc ggtcggccag cacccaggag cgacgccgtt cggtcgcgtc gtcgaaggcg
     6661 ccggtgccgg cgaggcggga ggcccggctc cgcagcgtct ggcgcagggg gtcgtcgatg
     6721 ccggcggcca gctcccgggc gtacacccgg agcaccggcg agacgttcgc cgggtcgtcg
     6781 gagtggagct cgccggcgag ccacgcggcc agctccagca cggacagccg gctgccccgg
     6841 cgggggtggg agtggggctc gagcacgatc gtcgacagat cgggcaggcc ggtggtccac
     6901 gcgccgtcgc tcgccgggac gtcgggttcg gcctcggtgc cagcaccggc cagctcgtcg
     6961 tcgagctcgt cgtcgcgcac cagcagcggt gccagcagct gccagccctc ccgtcgcagc
     7021 tcgtcgcgca gcgcggtggc aggcagccag tcggccgggt cgcccagcgc cagctcccag
     7081 cggtcctcgc cgatcgggcg caggtcgcga cccgcctcgc ccagcgtcgc cacgtggccg
     7141 gggccgagca cctggaaggc gaacacctcc gggacccgcc ggtccacgag gcggccggcc
     7201 acccggttcg gggatgcgcc cgagtcctcc gcccacggtg cgcccgacag gccggtgccg
     7261 aggtcgccga acgtgtcctc gaagtcgagg cacgcgtacg cgacgcggtc ctcgtcccgc
     7321 ccgcgggtcg cgggggcggc gtggtcgcgg gccacgtcgg cgaggagctc gaagcgggcg
     7381 agcagtccgg cgtcgtcgca gcgcggtccg ccggcggcca ccgccacgtg gggcgcgaca
     7441 ccgaagctga tggaggccgt ccgcacgcgg gtggtgacgt cgccggtgac cacgtcgcac
     7501 cacgcccgtg cccgggcgca cccgtggagg atcccggcgg cctccttggc catcaccgtg
     7561 gcctcgacgc cgagcacccg cagccggacc atcgtctcgg ggtcctggtc gccgaacacc
     7621 cactcgccgg cgaggtcgat ccagctcgcc ggcaggtggc cggcgctgcc ctgggggctc
     7681 gggaagaggc gcagcacgac ggcccggggg cactcgtcga ggtcggcgag gccgtccacc
     7741 tcgcacagga cggcggcgcc gtgctcgaag ccggcctcgg ccacgagctc gtcgacgatg
     7801 tcggcgacgc gccgccgggt gagcgggtcg gtggggaggt cgtcgagccg cacgatcggc
     7861 ccgtcgggca ggggtgccac gggcgacgtc cggggcggga cgaccccgtc gcggatggtg
     7921 cgccggacgg cctcggcgac ccgccggagg acgtcgacgc cccgggcgac gtcccgggtg
     7981 cgcaggtgca gcgcgaacga cccggcgacg gcgtccagct catcgagcag cgagtcggcg
     8041 gtggtcaccg ggcgggatcc tacgtccggg tcctcaccgg cgatccggtc gtgatccttt
     8101 cgagtgcatt tccccagcgg taacgttccc cacaccagca acacctccaa cacgcgcacc
     8161 cgacccggcg agagagcaca tgacgagcat cgaacacgtg gagggccgtg aggtcctcga
     8221 ctcccgtggc aacccgaccg tcgaggtcga ggtcgagctg gagtccggcg ccaccggtcg
     8281 ggccatcgtc ccctcgggcg cgtccaccgg ccagttcgag gccgtcgagc tgcgcgacgg
     8341 cggcgaccgc ttcggcggca agggcgtcct cgacgcggtc gacaacgtga acggcgacat
     8401 cgccgacgtc gtcctcggcc tcgaggctgc cgatcagcgc ggcgtcgacc aggccatgct
     8461 cgacctggac ggcaccgaga acaaggcccg cctcggggcc aacgccatcc tcggcgtgtc
     8521 tctcgccgtg gcccgcgccg ccgccgagga gcgcggcctt ccgctgttcc gctacgtcgg
     8581 aggcgccaac gcccacgtcc tgcccgcgcc gatgatgaac gtgctcaacg gcggcgccca
     8641 cgccgacaac aacgtggact tccaggagtt catggtgatg ccgctcggcg cagccagctt
     8701 ctccgaggcg ctgcgctggg gggtggagac ctaccacgcc ctcaagggca tcctccacga
     8761 ccgcggcctc agcaccgccg tcggcgacga gggcggcttc gcccccgacc tcggctccaa
     8821 cgaggaggcc gtgcagctgc tcgtcgacgc catcgagaag accggccgca cgccgggcca
     8881 ggacatcgcc atcgccctcg acccggcgaa ctcggagatc ttccgcgacg gcgagtacca
     8941 cctcgagggc gagggccgga ccctgtccgg cgaggggctg gccgagtacc tctcgagctg
     9001 ggtcgacaag taccccatcg tctccatcga ggacggcctc gacgaggagg actgggacgg
     9061 ctgggcggcg ctcaccgcct cgatcggcga ccggtgccag ctggtcggcg atgacctgtt
     9121 cgtcaccaac tccacccgcc tcgcccgggg catcgagtcc ggcgtggcca actcgatcct
     9181 cgtcaaggtc aaccagatcg gctcgctcac cgagaccctc gacaccgtcg gcctggccat
     9241 ccgcagcggc tacaccgcgg tcatgtccca ccgctccggc gagaccgagg acaccaccat
     9301 cgccgacctc gcggtggcca cgaactgcgg ccagatcaag accggcgccc cggcccgctc
     9361 cgaccgggtg gcgaagtaca accagctcct gcgcatcgag tccctgctcg gcgacacggc
     9421 gcgctaccga ggctgggacg cgctgtcccc cggtgtgcaa ccccggtgac cgcagccacc
     9481 cctgctcctc gcgccggcct ccggcgtccg cacctcccgt ccggcatcgg tcgatggatc
     9541 cggccgctgc tgctcgccac cgccgcggtg gtcaccatcg ccgcgctcgc cgtctacgtg
     9601 ttcccgaccc gcacctggct cgaccagcgc gccgcgctcg ccgagaccag cgtggagctg
     9661 cgcgagctcg aggccgagcg cagcgcgctc gagcagcgcg tcgccgagct cgactccgac
     9721 ggccagatcg aggagatcgc ccgctcccag tacggcctgg tgcggcccgg cgaggaggcc
     9781 tacgccgtgc tgcccgcgcc ggagaagccg gtcgagctgc cggccctgtg gcccttcggc
     9841 gacctgctcg agcccacgac cggggagccc accgcgccga ccggcgccga cgccggccgc
     9901 gactaggttc cccgcacgtc gacgtgctgc cccgctcggg ggcagggagg ggtaccgatg
     9961 agcatccttg gcaaccgtgt cctgcgcagc gaggacccga agttcctcac cgtcgggggc
    10021 agctacgtcg ccgacctgcg cgaccccctg ctggagggtg ccctccacgt cgcctacgtg
    10081 cgctcgatgg cggcccacgg caccatcacc ggcgtcgacg tcgaggaggc gcgcaacgcc
    10141 ccgggcgtcg tggccgtgtt caccggcgag gacctcggcg acctgccgga catgcccctc
    10201 gccgtcccgc tgctcaaccc gcagcacgcc cagccggtgc tggccaaggg caaggtccgc
    10261 ttcgtcggcg aggccatcgc cgccatcgtc gccgagacgc ccaccgccgc ggccgacgcc
    10321 gccgagctgg tctgggccga catcgacttc ctccccgccg tgctcacgcc cgaggacgcc
    10381 ctcgccggcg acaccctcct cttcgaggag tccggcacca acgccgccat cgagctgctg
    10441 ttcggcgagg acgacgccgc cttcgagggc tgcgaggtcg tcgtcgagca cgacttcctg
    10501 aaccagcggg tcgccggctg cccgctcgag gtccgctccg ccgccgccac ctgggacggc
    10561 gagcgcctcg tgcacttcgg ctcgacccag gcaccccacg gggtcaaggc caccctgcag
    10621 gccgtctacg ggctccacga gtccggcgtg cgcgtcgtcg ccccggacgt cggtggcggc
    10681 ttcggcccca agatcggcgc ctaccccgag gaggtcctcc tcgggtggct gtccaagcag
    10741 gtcggccgcc ccgtccgctg gaccgagacc cgctccgaga acatggtggc gatgggccac
    10801 ggccgcggcc agtggcagca gatcaagatc ggcggcaccc gcgacggcaa ggtcaccgcc
    10861 taccgcctca acctcaccca ggacgccggc gcctacgccg cgctcggcgc cttcctgccg
    10921 ttcctgaccc gcgtgatggc ctgcggcgtc tacgacatcc cggccgtgca gttcaacgcc
    10981 cggtcggtgc tcacgaacac gacgccggtg gtcgcgtacc gcggcgcggg ccggcccgag
    11041 gccaccgccg ccatcgagcg ggcgatggac ctcttcgccg ccgagatcga catggacccc
    11101 gccgaggtcc ggcgccgcaa cctcatcgcc gccgacgact tcccccacac caccgccacc
    11161 gacgcggtct acgacgtcgg cgactacgag cgcagcctcg acctcgccct cgacgccgcc
    11221 ggctacgacg atctccgcgc cgagcaggcc cgccgccgcg acgctggcga cccggtgcag
    11281 ctcggcatcg gcgtgtcggt ctacgtcgag gtcaccgcgg gcccgcaggc gggggaggag
    11341 ttcggccgcg tggtcgtcca ccccgacggg tcggccaccg cgttcaccgg ctccagcgct
    11401 cacggccagg gccacgacac cagcttcgcc atgctcgtca ccgacaccct cggcatcccg
    11461 ctcgagcagg tgacggtcaa gcacggcgac accgatgagg tcgcccgcgg cgtgggcacg
    11521 ttcggctccc gctccctcca gctcggcggg tcggcgatct tccaggcatc gggccaggtg
    11581 ctcgacaagg cccgcgagct cgccgccgag ctgctcgagg ccgacccggc cgacatcgag
    11641 ctcgaccggg ccgccggcgc cttccacgtg cagggcacgc cggccgtgtc gcggtcgtgg
    11701 gccgacgtgg cctcggcggc cggcgagagc ggactggcgg ccgagaccga cttcaaggcc
    11761 gacagcccca cgttcccgtt cggatcgcac gtcgcggtgg tcgaggtcga caccgagacg
    11821 ggcatggtcc ggctccagcg catgatcacc tgcgacgacg ccggccggat cctcaacccg
    11881 atgctggtcg agggccagcg ccacggcggc atcgcccagg gcgtggccca ggcgctctac
    11941 gaatgggtgc gctacgacga cgacggcaac ccccagaccg ccaacctcgc cgactacacg
    12001 atgatctcgg cggccgagct gccgagcttc gagctggtca gcatggagac ggccacgccg
    12061 ctcaacccgc tcggcgccaa gggcatcggc
//
LOCUS       DATISU010000138         6515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_519044, whole genome shotgun sequence.
ACCESSION   DATISU010000138 DATISU010000000
VERSION     DATISU010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519044"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VLR27_05720"
     CDS             <1..999
                     /locus_tag="VLR27_05720"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HSP02978.1"
                     /translation="EISAAITTVLRPGTLVGASASSVLAGAQEAEEVPALSVWAAWTE
                     PVRPLRLATGPVHDDGDGPAVMVQGVPADVPLGCTILLLADPHSFPADGFLTQLAEAR
                     PDIVVVGGLASAASQPGGNALVLDGEVHRDGAVGVVLPPTQAVVSQGCRPVGEPMVVT
                     QADRGFLIELAGRPALDRLTDLTTDLPVEDRALLAKGIQLGVAIDEHRLEFGRGDFLV
                     RPVLGADRGRGVIAVGTEIAVGTTVQFHVRDAASADDDLWQLLDGVTGAGALVFTCNG
                     RGERLFGVEDHDAAVVSEALDTTAVAGMFCAGELGPVGGRSFLHGFTASILVFADR"
     gene            1097..3058
                     /gene="tkt"
                     /locus_tag="VLR27_05725"
     CDS             1097..3058
                     /gene="tkt"
                     /locus_tag="VLR27_05725"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HSP02979.1"
                     /translation="MRDADLEKRAINVIRGLAMDAPHAASSGHQGTAMALAPLAHVLW
                     TRVLRYDAADPDWPDRDRFVLSAGHASILQYSMLHLTGHGLELDDLKAFRQWGSRTPG
                     HPEVGHTRGIEVTTGPLGQGFANAVGMAMAERWLAARFGAELFDHHTFVIAGDGDLAE
                     GLSHEAASLAGHQGLDKLVVIYDDNHVSIDGPTELSLSDDAATRFRGYGWNVDEIGEV
                     AEDLDALEAALERAKANTGAPTIIILRSHIGYPSPTLTDSPKAHGLAFDEDTIREAKE
                     VMGLPDEPFHVPDDVLTYYRSAGERGRSEREEWEKRLAAFDGERAALDCCLDAEGLPG
                     WEKALPTFGLDEAMATRQASGKVLQALTETVPGLVGGGADLTGNTGTAIADAGVMSAD
                     EPGGRQIFFGVREHGMAAIMNGMALHGGLLPVGGTFLVFSDYARGAVRLSALSGAKVI
                     YSFTHDSVGVGEDGPTHQPVEHVAALRAMPGLSVIRPADANETVAAWRAAIDGHGPTA
                     LILSRQSVPTLEGTSADGVLKGGYVLVDADGEPDLVLVATGSEVHVCVEAARRLTEEG
                     VAVRVVSLPSWNLFEAQSDDYCDQVLPPDVPTLAVEAGVSFGWDRYADDSITIDHFGH
                     SAPGELVLEKLGFTPENVVERSRELLASS"
     gene            3069..4169
                     /gene="tal"
                     /locus_tag="VLR27_05730"
     CDS             3069..4169
                     /gene="tal"
                     /locus_tag="VLR27_05730"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HSP02980.1"
                     /translation="MGLLHDLHENGGQSPWLDNLRRGYLRSGELQAYVDRGVRGITSN
                     PSIFQKAIADSDDYDEQFSSLLGSGSSVDDAYWRMVTDDIGEALEVLRPVYDASDGVD
                     GYVSVEVDPGLASDTDGTIESARHLHETIDAPNVLVKIPATAEGVPAIRTMLAEGRSI
                     NVTLIFSIDRYAEVMEAYLAGLETHAASGAEDLSKIASVASFFISRVDTEVDNRLEAI
                     GTDEALALRGKVAVAQAQLAYQRFQEVFSGPRWEALAAKGARVQRPLWASTSTKNPEY
                     PDTLYVDTLIGPDTVNTMPEATLEAFDDHGTVARTVDADFAAAQDVVDRATAVGVDLD
                     DVSRVLEDEGVAAFSKSFDELITTLETKADQL"
     gene            4172..4831
                     /gene="pgl"
                     /locus_tag="VLR27_05735"
     CDS             4172..4831
                     /gene="pgl"
                     /locus_tag="VLR27_05735"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HSP02981.1"
                     /translation="MRGELRTVDDVAATFCDVVADAWESRTGDLFTIALSGGDTARRC
                     YEALARHADTPVDWSEVRVVWGDERCVPLDHEDSNHRLAQESLLRSVGPVAEIHPMQC
                     DVADAYDRLIAGIDIDVIHLGLGPDAHTASLFPQSPALDAPDDRFVVTNEDPLGNNPR
                     PRLTFTTTAIAAGRTVVVTVEGESKREALDRVRAGEDVPAARIDRPGVVWLTDRAAAA
                     S"
     gene            4841..6388
                     /locus_tag="VLR27_05740"
     CDS             4841..6388
                     /locus_tag="VLR27_05740"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSP02982.1"
                     /translation="MDERRTPVLRADEDLRQTARDVRMRLLASISLLHSGGGLFVFGY
                     IQLRYPASEAYDTPWINDWLLVLLTLAALAPVVWGWVAHEFRRSSGWALAGRTPDPDD
                     REHLLTAPFRLAAKPLGFWLAAALVIGAGIGVRRVFGFREIFDIAQILLMGGLATCAI
                     SYLVIERAFRPLFAGALTGTDISRPRTLGVRARLLLAWAASSGVPLLGLTLSPFREAA
                     TSNAALVALGLIGLVAGLLAVSVASDTIAVRLDGIRSALARVGSGDLSQDLLVDDGGE
                     VGQLQAGFNQMVHGLRERQQLQDLFGRHVGQEVAAQAMERGSDLGGESRRASVFFVDL
                     IGSTAMAEVLPPGEVVSTLNAFFEAVVEEASAEGGWVNKFQGDGALCVFGVPAHHPDH
                     EARALRAARALRDRLVELSLDHPGLHAGIGVSSGTVVAGNVGSQERFEYTVIGRAVNE
                     AARLTELSKSRPGRALAAASSVQAAGTESSNWAARGTVGLRGQSAPTHVYEIATGVGT
                     GSRHVLR"
BASE COUNT          856 a         2568 c         2171 g          920 t
ORIGIN      
        1 gagatctccg ccgccatcac caccgtcctc cgtcccggga cgctggtcgg ggcctcggcg
       61 tcgtccgtcc tcgccggcgc ccaggaggcc gaggaggtgc cggcgctgtc ggtgtgggcg
      121 gcctggaccg aaccggtgcg cccgctacgc ctcgccaccg gccccgtgca cgacgacggc
      181 gacggtccgg ccgtgatggt gcagggcgtg cccgccgacg tgccgctcgg ctgcaccatc
      241 ctgctcctgg ccgatcccca ctcgttcccc gccgacgggt tcctgaccca gctcgccgag
      301 gcccgccccg acatcgtggt cgtcggcggc ctcgcctcgg cggcctcgca gcccggcggc
      361 aacgccctgg tgctcgacgg cgaggtccac cgcgacggtg ccgtcggggt cgtcctgccg
      421 cccacgcagg cggtggtcag ccaggggtgc cggccggtgg gcgagccgat ggtggtcacc
      481 caggccgacc gcggcttcct gatcgagctg gccggccggc ccgccctcga ccgcctcacc
      541 gacctcacca ccgacctccc ggtggaggac cgagccctgc tcgccaaggg catccagctc
      601 ggcgtcgcca tcgacgagca ccgcctggag ttcggccggg gcgacttcct cgtccgaccg
      661 gtgctcgggg ccgaccgggg ccgcggcgtc atcgccgtcg ggaccgagat cgctgtcggc
      721 accaccgtcc agttccacgt gcgcgacgcc gccagcgccg acgacgacct gtggcagctg
      781 ctcgacggcg tcaccggcgc gggcgccctg gtcttcacct gcaacgggcg cggtgagcgc
      841 ctgttcgggg tggaggacca cgacgccgcc gtggtctccg aagccctgga cacgacggcg
      901 gtggccggca tgttctgcgc cggcgagctg ggcccggtgg gcgggcgcag cttcctccac
      961 ggcttcaccg cgtcgatcct ggtcttcgcc gaccgctgat cggtctccgc ccggggaggc
     1021 tccggaggtc accgagtatg gtcgacccct cactgccccc gggcagccct cgaccgtccc
     1081 ccacctggag cgatcagtgc gcgacgccga cttggagaag cgggccatca acgtgatccg
     1141 tggcctggcc atggatgccc cccacgctgc gagcagcggc caccagggca ccgcgatggc
     1201 gctggctccc ctcgcccacg tgctgtggac ccgggtgctg cgctacgacg ccgcggaccc
     1261 ggactggccc gaccgcgacc gcttcgtgct ctccgccggc cacgccagca tcctgcagta
     1321 ctccatgctc cacctcaccg gccacggcct ggagctggac gacctgaagg cgttccgcca
     1381 gtggggctcg cgcaccccgg ggcaccccga ggtcggtcac acccggggca tcgaggtcac
     1441 caccggcccg ctcgggcagg gcttcgccaa cgccgtcggc atggccatgg ccgagcgctg
     1501 gctggccgcc cgcttcggcg ccgagctgtt cgaccaccac accttcgtca tcgccggcga
     1561 cggcgacctg gccgagggcc tgagccacga ggccgcgtcg ctggccggcc accagggcct
     1621 cgacaagctc gtcgtgatct acgacgacaa ccacgtgagc atcgatgggc ccaccgagct
     1681 gtccctgtcc gacgacgccg ccacccgatt ccggggctac ggctggaacg tggacgagat
     1741 cggcgaggtc gccgaggacc tcgatgccct ggaggccgcc ctcgagcggg ccaaggccaa
     1801 cacgggcgcc ccgaccatca tcatcctgcg cagccacatc gggtacccct cgcccaccct
     1861 caccgactcc cccaaggccc acggcctcgc cttcgacgag gacaccatcc gcgaggccaa
     1921 ggaggtcatg ggcctgcccg acgagccgtt ccacgtgccc gacgacgtcc tcacctacta
     1981 ccgcagcgcc ggcgagcggg gccggtccga gcgcgaggag tgggagaagc gcctcgcggc
     2041 cttcgacggc gagcgcgccg cgctcgactg ctgcctcgac gccgagggcc tccccggctg
     2101 ggagaaggcg ctgccgacct tcggcctcga cgaggccatg gccacccggc aggcgtcggg
     2161 gaaggtcttg caggccctga ccgagaccgt ccccggcctg gtcggcggcg gcgccgacct
     2221 caccggcaac accggcaccg ccatcgccga cgccggcgtg atgagcgccg acgagcccgg
     2281 cggtcgccag atcttcttcg gcgtccgcga gcacggcatg gctgcgatca tgaacggcat
     2341 ggccctccac gggggcctgc tcccggtcgg cggcacgttc ctcgtgttct ccgactacgc
     2401 ccgcggcgcc gtgcgcctct cggccctgtc cggcgccaag gtcatctact ccttcaccca
     2461 cgactccgtg ggcgtcggcg aggacggccc cacccaccaa ccggtggagc acgtcgccgc
     2521 cctgcgggcc atgcccggcc tgtcggtgat ccgtccggcc gacgccaacg agacggtcgc
     2581 cgcctggcgt gccgccatcg acggccacgg cccaaccgcg ctgatcctga gccgacagtc
     2641 cgtcccgacg ctcgagggca cctcggccga cggggtcctc aagggcggct acgtcctggt
     2701 ggacgccgac ggcgagcccg acctcgtgct ggtcgccacc ggctccgagg tccacgtctg
     2761 cgtcgaagcc gctcgccgcc tcaccgagga gggcgtcgcc gtgcgcgtcg tgtcgctgcc
     2821 gtcgtggaac ctgttcgagg cccagagcga cgactactgc gaccaggtcc tgccgcccga
     2881 cgtgcccacc ctggccgtcg aggctggcgt cagcttcggc tgggaccgct acgccgacga
     2941 cagcatcacc atcgaccact tcgggcactc ggcccccggc gagctcgtgc tcgagaagct
     3001 cggcttcacc cccgagaacg tcgtcgaacg cagccgcgag ctcctggctt cctcgtagag
     3061 tcggcctcat gggcctgctc cacgatctgc acgagaacgg cggccagtcc ccctggctgg
     3121 acaacctgcg gcggggctac ctccgctccg gtgagctcca ggcctacgtc gaccggggcg
     3181 tccgcggcat cacctcgaac ccgtccatct tccagaaggc catcgccgac agcgacgact
     3241 acgacgagca gttctcctcg ctcctcggca gcggcagctc cgtcgacgac gcgtactggc
     3301 gcatggtcac cgacgacatc ggcgaggccc tcgaggtcct ccgcccggtg tacgacgcat
     3361 ccgacggcgt cgacggctac gtctcggtcg aggtcgaccc cggcctggcc agcgacaccg
     3421 acggcaccat cgagtccgcc cgtcacctgc acgagaccat cgacgccccc aacgtgctgg
     3481 tgaagatccc ggccaccgcg gagggtgtgc cggcgatccg cacgatgctg gccgagggcc
     3541 gctcgatcaa cgtcacgctc atcttctcca tcgaccgcta cgccgaggtg atggaggcct
     3601 acctggccgg cctcgagacc cacgccgcct ccggcgccga ggacctctcg aagatcgcca
     3661 gcgtggcgtc gttcttcatc tcccgggtcg acaccgaggt cgacaaccgc ctcgaggcca
     3721 tcggcaccga cgaggccctc gccctgcgcg gcaaggtcgc cgtggcccag gcccagctcg
     3781 cctaccagcg gttccaggag gtcttctccg gtccccgctg ggaggccctc gccgccaagg
     3841 gtgcccgggt ccagcgcccg ctgtgggcat cgacctccac caagaacccc gagtaccccg
     3901 acacgctcta cgtcgacacc ctcatcgggc cggacacggt caacaccatg cccgaggcca
     3961 ccctcgaggc attcgacgac cacggcaccg tggcccgcac cgtcgacgcc gacttcgccg
     4021 ccgcccagga cgtcgtcgat cgggcgaccg ccgtcggcgt cgacctcgac gacgtgtccc
     4081 gcgtgctcga ggacgagggc gtcgccgcct tcagcaagtc cttcgacgag ctgatcacca
     4141 cgctcgagac caaggccgat cagctctagc catgcggggc gagctgcgca ccgtcgacga
     4201 cgtcgccgcc acgttctgcg acgtcgtcgc cgacgcctgg gagtcccgca ccggtgacct
     4261 gttcaccatc gcgctctccg gcggtgacac ggcccggcgc tgctacgagg cgctggcccg
     4321 ccacgccgac acgcccgtcg actggtccga ggtccgggtc gtctggggtg acgagcgctg
     4381 cgtgccgctc gaccacgagg actcgaacca ccggctggcc caggagtccc tcctccgctc
     4441 ggtcggcccg gtcgccgaga tccacccgat gcagtgcgac gtggctgacg cgtacgaccg
     4501 cctgatcgcc gggatcgaca tcgacgtcat ccacctcggt ctgggtccgg atgcccacac
     4561 ggcgagcctg ttcccccagt cccctgccct cgacgccccc gacgaccggt tcgtcgtcac
     4621 caacgaggac ccgctcggca acaacccccg tccccggctc accttcacga ccacggccat
     4681 cgccgccggt cgcacggtgg tcgtcaccgt cgagggggag tccaagcggg aggccctcga
     4741 ccgggtccgg gccggcgagg acgtgccggc ggcgcgcatc gaccggcccg gcgtggtgtg
     4801 gctcaccgac cgagcggcgg ctgcctccta gcctgcaccc atggacgagc gccgcacccc
     4861 cgtgctgcgc gccgacgagg acctgcgcca gaccgcgcgc gacgtgcgga tgcgcctgct
     4921 cgccagcatc tccctgctcc actcgggcgg cgggctcttc gtcttcggct acatccagct
     4981 ccggtacccg gcgtcggagg cctacgacac cccgtggatc aacgactggt tgctggtgct
     5041 gctcacgctc gccgccctgg cgcccgtggt ctgggggtgg gtggcccacg agttccgccg
     5101 cagctcgggc tgggccctcg ccggtcgaac gccggacccg gacgaccgcg agcacctgct
     5161 caccgcaccc ttccgcctgg ccgcgaagcc gctcgggttc tggctcgccg ctgccctggt
     5221 catcggtgcg ggcatcggcg ttcggcgggt cttcggcttc cgggagatct tcgacatcgc
     5281 ccagatcctc ctcatgggcg ggctggcgac gtgcgcgatc tcgtacctgg tcatcgagcg
     5341 ggcgttccgc ccgctgttcg ccggtgcgct gaccggcacc gacatctccc ggcctcgcac
     5401 cctgggcgtc cgggcccgcc tcttgctcgc ctgggccgcg tcctccggcg ttccgctcct
     5461 cggcctcacc ctcagcccgt tccgcgaggc cgccacgtcc aacgccgccc tcgtcgccct
     5521 cggcctcatc gggctggtgg ccggactgct cgccgtgtcc gtcgcctccg acaccatcgc
     5581 cgtccgcctc gacggcatcc gctcggcgct ggcgcgcgtc ggctcgggcg acctgtccca
     5641 ggacctcctc gtcgacgacg gcggtgaggt cggccagctc caggccgggt tcaaccagat
     5701 ggtgcacggc ctgcgggagc gccagcagct ccaggacctg ttcggccgcc acgtcggcca
     5761 ggaggtcgcc gcccaggcga tggagcgagg cagcgacctc ggtggcgagt cccgccgggc
     5821 gtcggtgttc ttcgtcgacc tgatcggctc caccgccatg gcggaggtcc tgccgcccgg
     5881 cgaggtggtg tccacgctca acgcgttctt cgaggcggtg gtggaggagg ccagcgccga
     5941 gggcggctgg gtcaacaagt tccaggggga cggtgccctc tgcgtgttcg gcgttcccgc
     6001 ccaccatccc gaccacgaag cccgtgccct gcgcgccgcc cgggccctgc gggaccggct
     6061 cgtcgagctg tccctcgacc accccggcct gcacgcgggc atcggcgtct cgtccggcac
     6121 ggtcgtcgcc ggcaacgtcg gcagccagga gcgcttcgag tacacggtga tcggccgggc
     6181 ggtgaacgaa gcggcccgcc tcaccgagct gtccaagtcc aggccgggcc gggccctcgc
     6241 ggccgcctcg tccgtgcagg ccgccggcac cgagagctcc aactgggccg cccggggcac
     6301 cgtgggcctg cgcggccagt ccgcccccac tcacgtctac gagatcgcca ccggtgtggg
     6361 aactggcagt cggcacgtgc tgcgctgacc gaagcaatcg ccatggcgcg ccgtcagggc
     6421 cggcctgccg accgggattc gacgctgaca cccgcggtcg gtcagacggg tggggtgatg
     6481 ggtccgtcgt cgtcggggcg gtggatggtc caggt
//
LOCUS       DATISU010000139        16927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_797902, whole genome shotgun sequence.
ACCESSION   DATISU010000139 DATISU010000000
VERSION     DATISU010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 16927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16927
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797902"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..2012)
                     /locus_tag="VLR27_05745"
     CDS             complement(201..2012)
                     /locus_tag="VLR27_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSP02983.1"
                     /translation="MVGLMIVGWAVAAFAAAVAFRFRRHLGAATHESDTLHHQLAASE
                     AFSRSLVEATDEAILTIREDGIVGSANHAATRLLGLAASAIVGRPAESLAERFPLLAD
                     LPVGLTDADTVVHHPSARRAVSVTTRATGDEDDPVIAVFARDISERKILEEQLAHQAT
                     HDDLTGLANRASLVAELDRAAARAERSGQGLGLLFIDLDRFKSVNDTLGHRVGDVVLQ
                     QVAARLLQATRDTDLVARNGGDEFVVLAEEAGDVTVVARLAERIQDALSEPVAFDDDV
                     IPVSASIGVAWLEPGDPHTLDQLRDADVAMYHAKSSGPGQIRVFDDDMRLAVEQRFDL
                     ERELRRAVGIGALDACIQPVLDLRTGQIVGGELLCRWTTEDGREVPPEAFIEVAEDST
                     LVVEIGRWMLDRAGSVLGEWALDRTQGLADLHLSVNLSGRHVDHPGVVEDVTSLWDRW
                     GIQPSQLIVEITETSLLKDLDEAARTLEQLREAGVTICVDDFGVGYASLRYLRELPVG
                     LVKIDRSIVSGFGRIDSDTVIVTMLSRLADVLDIQIVAEGIETEEQRERLTEIGCHFA
                     QGYLFAKGMPVEAFPLWLARWRRRHTHPSAWRAVVPG"
     gene            complement(2078..2386)
                     /locus_tag="VLR27_05750"
     CDS             complement(2078..2386)
                     /locus_tag="VLR27_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP02984.1"
                     /translation="MRTTCPSCDTVLIDAGALTVRRRPDATQSECCFICPDCVQAVVQ
                     PVSDKMVPVLIGAGCMVEDWGVSDARFLHPSGGGITEAEIQDFVSSLDRDDWFGELLS
                     "
     gene            2570..3445
                     /locus_tag="VLR27_05755"
     CDS             2570..3445
                     /locus_tag="VLR27_05755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HSP02985.1"
                     /translation="MSHLTIPAATDHPAPGAPLGPGSLLWRIAADWRSGLSGLSAGIL
                     QLAWPHLGQGVEEHSAFFDEPWDRIHRSVPRIWATIFADDADERGRDIRDLHKGIKGT
                     DPHGQRYHALDPETFWWAHATFTWEMFQTADEWDHRTLSARRREQLYQETVTWYRRYG
                     VSDRPVPADHAAFEARFDEICRTQLELTPTAERAIDMALHDRVSPPGMPGPVATVATP
                     LMRTMALGGLPPVVRARFGIPWTMADELALAGFRQLVRDAGRVVPHRLTRGPYLRSIK
                     RMKHIAAPRPEQIPA"
     gene            3447..4076
                     /locus_tag="VLR27_05760"
     CDS             3447..4076
                     /locus_tag="VLR27_05760"
                     /inference="COORDINATES: protein motif:HMM:NF037271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HSP02986.1"
                     /translation="MPKVATQVDAVAVLTALAQRPVDTEADDVDRRILDAAGRLLVDV
                     GLEELEVDQVAELAGVGRSTIYRRFDGRNALLAATVAHEARRLLAALADAVAGIDDPE
                     EQLVVAFSTGLRVARATGLAERVRDEPLLLRLITVDGGPVLAAASTQLAGFAQALDPT
                     VHERDARETAELLVRLAISLVLTPETALDLEEGCEETVRRRIAPLLRRR"
     gene            complement(4073..4585)
                     /locus_tag="VLR27_05765"
     CDS             complement(4073..4585)
                     /locus_tag="VLR27_05765"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HSP02987.1"
                     /translation="MTAQSPAIIARDQGDRFHFLGNLYTAKLDGEHTDGAMTVMEFLA
                     PRGFGPPLHRHDVEDELFYVIDGEMWFACGDVEAVHTTGAVVWLPRGRPHTFQVRSDT
                     ARVLQVSTPAQFERFVATLGRPTDDTGLPEPEEIDPAHVAEVCAQFSIEVLGPPPDPA
                     LAGASAGAAR"
     gene            4717..5790
                     /locus_tag="VLR27_05770"
     CDS             4717..5790
                     /locus_tag="VLR27_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSP02988.1"
                     /translation="MGRAGIASFTTARDLLVAAADRVGTGEEACTEIARAFTHVMRHD
                     GAAVMTTDPDTHLPAGGIVSGFDSSACVPFWDNELLDPDFHKFNELARSTERVATLAD
                     ATDGDLRRSPRFQKLYEGFGVSDEMRVAFVAGSTCMAIGAFVRCDGDVYSPTEVRDVK
                     NLLAPATNVLRRALGCTREPLTAHGPVVVLLDRDGQICGRSEGADAVLDDLRIRVDDD
                     LPGTIMVAAARAQASRSSTRLTTRLRGRSGRWVRIHVAPMEGSDGTVSVTIDAARAGD
                     LVPILLDSYGLSSRESDIVLLVCRGVGTKEIASELSISVHTVRDHLKAIFDKAGVNSR
                     GELVAQLFSDHVLADFHRTIAHV"
     gene            5993..7684
                     /locus_tag="VLR27_05775"
     CDS             5993..7684
                     /locus_tag="VLR27_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSP02989.1"
                     /translation="MQRHRSTTWTTRSWVLLAVLAMFATACGGNDDDGGDAAPGTEGD
                     NGAETTETTVEDAGDPVEGGSITVGLEAETNNWLPGQGNFSNPGINVALAIYDPLMRR
                     NPEGEPTPYLAESLEPNEALDEWTLTLRPDVVFHDDTPLNAEAIKTIFDDYIKADDSN
                     LAGQVVDVESVDVVDDLTVVYRLSGPNAAFASVLTGAVGWPFSPTAAAEAGADAGSRP
                     VGTGPFVFGEWQRDNRLTVTKNESYWQQGLPYLDQIVFRPIPDEDTRISSLGTGDIDV
                     LQSLRQSAIVRARDVQDVVSHEHVGNNSGSAIFNTAQPPLDDVRVRRALAYALDQDQL
                     IDVLGGAGITPPQTQYFGDESPFYSEAAAEEWPTNDPEQAMQLIDEYTSDPNRSDGKA
                     AGDPVTFVFDCPPDPSLIELSQLYQAFWESIGVQVDLNQVEQATHINEALAGDFDSKC
                     WRVSDEDDPYIIFSEAFGPESPLNFTNFTDPAITEQLEVLRTTTDIDERKAAVEEISL
                     VLAENVPNTFTAGTLTTLAARDAVKNIPGWTFPDGSEGEGVIGGTTMWGHVWLAE"
     gene            7728..8663
                     /locus_tag="VLR27_05780"
     CDS             7728..8663
                     /locus_tag="VLR27_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP02990.1"
                     /translation="MLRRLPPLIGTLIAVSILTFLLTALLPGDPAVQILGAQSATPEA
                     VAQVRAELRLDQPLPNQYWHWISDAVTGDFGRSYRTGQEVSEAIVERLPVTLQIGAMA
                     IGFALLFAVPLGMLSAYRAGGALDRGVTGGTFTLLAIPNFMMAILLILIFAEILGWLP
                     ATGWVRLSDDPAANLRAALLPALSITMAELAVYTRLLRSDMISTLQEDYIAVSRAKGM
                     PTWWILLRHALRPSSFSLLTVVGVQIGAIISGSVIIETLFALPGVGRLLVDSIFQRDL
                     IMVQGIALVVAVSYVIVNFIVDILYSYLDPRIRRG"
     gene            8656..9615
                     /locus_tag="VLR27_05785"
     CDS             8656..9615
                     /locus_tag="VLR27_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP02991.1"
                     /translation="MAEPPAHEPEHTELGALAGQAGEPGEDGMGTITVVDTATASVPA
                     APARKEGKGFAFWLAVAWLVVVAICAVFAEQLPVADEVRPNVIDKLATPSADYPLGTD
                     GLGRDTLARLVHGARVSVVVSLSAVGVGMFVGGTLGMIAGYVRGWFETLLMAAVNVIL
                     AFPGLVLLLVLLAYVGQSLGVISLVIGVLSIPIYTRVARANTLAVAQREFVLAAHTMG
                     ANRLRILFREIAPNVIMPVLAFGLVAMGVIIVLEGALAFLGLSVEAPTPTWGGMIAEG
                     KRHLSTDPHVALIPSAAMLLTVLSLNFVGDVLRRGYDVREANL"
     gene            9612..10733
                     /locus_tag="VLR27_05790"
     CDS             9612..10733
                     /locus_tag="VLR27_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP02992.1"
                     /translation="MIFRKGGRDKSFEPVEGAPLLEVRDLHTRFQTPRGMLHAVDGVS
                     FTLERGRTLGIVGESGSGKTVLARSVMNLVFSRKMEREGSVLYRGRDLHEMSMREMYD
                     VWGLEIASVFQDPMTSLNPTLRIGDQLAESLWYHLDMRGSELHEMQVALLRSVGIPEP
                     ERRLDVYPHELSGGLRQRVTIAIALACGPHLLVADEPTTALDVTVQKQILDLLDRQQK
                     ERYMGMILITHDLGVVAGRADEIAVMYSGQIVELGTTRALFHERRHPYTDGLLRSIPR
                     LSNVPHSKLSVIPGRPPDPVNRPAGCRFAPRCPHAQPRCLEQVPELRSDEQGDDPNHQ
                     FACFYPVGTPEGDDAHATNLARGTNAAGTPVGDETEVPA"
     gene            10733..11734
                     /locus_tag="VLR27_05795"
     CDS             10733..11734
                     /locus_tag="VLR27_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HSP02993.1"
                     /translation="MAAGGTETPQLRDRDDIVLSVEHLVVEFPTPNGTVHAVSDVSFD
                     VAAGETLGLVGESGCGKSTTARAIMQVPPPTSGHIFYEGTDLGSLDRKELRRTRPKLQ
                     MIYQDPVSSLNPRRKVRAIVGEGLSIWRRDRADEIVEEVMRAVGIDPELAGDRHPHEF
                     SGGQCQRICIARSLALDPSVLICDEPVSALDVSVQAQILNLLEEMKRRYELTMLFVAH
                     DLAVVKNISDRVAVMYLGKLCEIGPSVDVYERPAHPYTRALMAAVPEPDPDVVLDEDE
                     GLAGELPSPISPPSGCRFRTRCPLAEDRCAEEAPEMREVGPGHHVACHFPLVEPAET"
     gene            11859..13421
                     /locus_tag="VLR27_05800"
     CDS             11859..13421
                     /locus_tag="VLR27_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSP02994.1"
                     /translation="MHTTEHGVRLQYGGRARQAHDVVLVVDDEDAMRAAFEAALRDDG
                     FTVFSASTGAEALVLAVEVEPTVVLLDANLPDMRGEDVLVELRRSNGSEQPAVLIVSG
                     DRQLDRKVEGLRLGADDYITKPVALAELVARVRRHAGSRHRWLAQLDDVLDDRRRLAQ
                     RIAGLDSSAPLHLLAAELFGIFAEQLTCDALSLTSALDGSVQHTVLAPSATRFRAALE
                     RGAGAGARRPMIDRGEQGWTCHAPLEITGSAFGVLGVAGTGDEQGTLSAVVDLAPQIA
                     GLVHRALTSDTAVAGHRRQVEAVLSDDAFWAEFQPIVDLRSGSTVGYEALTRFPDGRR
                     PDLWFRQAAGVGLGADLELAAIERVLDTSHLLPGDTWLSINVSAHTLIEHDLRPVLAR
                     ADRRVILEITEHERVRDYRAITRSMQGLGDAGLGVDDAGSGYASLRHIFELQPELVKL
                     DRTWIHGMHQDPVRTALITGLLHFATELDASLLGEGIEHAEDRAALERTGVTLGQGYL
                     FGRPRPAEMWTS"
     gene            complement(13438..15747)
                     /locus_tag="VLR27_05805"
     CDS             complement(13438..15747)
                     /locus_tag="VLR27_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSP02995.1"
                     /translation="MTRPSDDEERSAALRLEQSEALRRLAGRLARVGGWRIDGADRTV
                     YWSDELHDILESPSHEGVPALDDAIERYPDAHRETISEALERCLRDGTPFDVELEIDT
                     FAGRRIPVRIIGEPDRDSEGEVCGVMGAFQDISDLRMATQEAQDAARRLGSTLESMTD
                     AVFMVDRDWRFTYLNGNAEQLLRRDADDLLGRGLWEEFPQAAETEVHEVYTASMADRR
                     TRHVDEFHFPPLDTWFQVDVYPADQGLAVYFRDITDARAARVALEQREQVLQRQADLL
                     DQAQDAIIVRDPGGRITYWNRSAERIYGWFAEEALGSDVRSLLYEDRGAFDAAHEAVL
                     RTGRWSGELRQRTRRGDLVEVAARWTLVRNPSGSPQAVLAINTDITERKRVEQQLLRA
                     QRMESIGTLAGGVAHDLNNVLAPILLAGELLQSDSVNIDRSTLVDTIVTSARRGADLV
                     SQVLSFARGVEGERRPVEVGSLVTDIGAIVRNTFPKNISLTLDVAASLAPIVGDSTQI
                     QQVLLNLVVNARDAMPDGGHLRLAASEVVIDDEYVATAPGAKAGSYLLVEVEDDGEGM
                     APEVRDRAFEPFFTTKAHGVGTGLGLSTAVAIIESHDGFLQVYSEPDRGTRFRVYLPC
                     DLAPQEQPATPRADTDPPRGSGQSVLVVEDEAAIRAMTRQTLETYGYRVLEAANGAEA
                     VAVFAREAGGVDVVLTDMMMPVLDGPATIHALQNLDPDVRVIGASGLHGNGKVAQASA
                     AGVRHFLPKPYETRRLLEVLAEVLGDPAS"
     gene            15861..16343
                     /locus_tag="VLR27_05810"
     CDS             15861..16343
                     /locus_tag="VLR27_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HSP02996.1"
                     /translation="MLTGASKLETTQMLASLPLFAGLTKRQLGAVSKAVDHASFEPGT
                     ELLTELKEVQRLIIIRSGTAAITRRGAVAGDGGGLQAGTSRRLGTVGPGDVVGELSLI
                     DGHVASASVVAETPIDAVLLYRTRFNSLLAATPKLYPRLLVGMATRIRAMDRHTDMIS
                     "
     gene            complement(16336..>16927)
                     /locus_tag="VLR27_05815"
     CDS             complement(16336..>16927)
                     /locus_tag="VLR27_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HSP02997.1"
                     /translation="GNVEVSQRVADVVLAALAASDPERVAAGNQGTMNNLLIGGRGWV
                     YYETVGGGQGGRMAEGSPAPGQSGIQTGMTNTKNTPIEALERHFPLRVLRYRLRRGSG
                     GAGLAPGGEGIERDLQVLEDVTVSLITERRVSRPNGLAGGAPGATGENWLLPQGDEAR
                     VERLPDKCTIRLQAGDVLRMLTPGGGGWGTPPDGVS"
BASE COUNT         2453 a         6233 c         5753 g         2488 t
ORIGIN      
        1 gatgacgacg tgctggccct tctcggccag gaggcgtgcg gtggcttcgc cgaggccgct
       61 ggccccgccg gtgatgacag atgaagaacc ggtgatgtcc atggccgggg agactattga
      121 ctggtcggtc aagttcgcca atgtcccgca tcaaccggac gaatgcgcag cattcgtcga
      181 cgaacccgaa gggttcgcgg tcagcccggg accacggccc gccacgcgct ggggtgggtg
      241 tgccggcgac gccaccgggc cagccagagg gggaaggcct cgaccggcat gcccttggcg
      301 aagaggtagc cctgggcgaa gtggcagccg atctcggtga ggcgctcgcg ctgctcctcg
      361 gtctcgatgc cctcggcgac gatctggatg tcgagcacgt cggccaaccg gctgagcatg
      421 gtgacgatga ccgtgtcgga gtcgatgcgg ccgaagcccg acacgatgga ccggtcgatc
      481 ttcaccaggc cgaccggcag ctcccgcagg tagcgcaacg aggcgtagcc cacgccgaag
      541 tcgtcgacgc agatggtgac cccggcctcg cgcagctgct cgagggtgcg ggccgcctcg
      601 tcgaggtcct tgagcagcga ggtctcggtg atctccacga tgagctggga cggttggatg
      661 ccccaccggt cccacaggct ggtgacgtcc tcgacgacgc cggggtggtc gacgtggcgg
      721 cccgacaggt tcacgctcag gtgcaggtcg gcgaggccct gggtgcgatc cagagcccat
      781 tcgcccagca cgctgccggc ccgatccagc atccagcgac cgatctcgac caccagggtg
      841 gagtcctcgg cgacctcgat gaacgcctcg ggcgggacct cgcggccgtc ctcggtggtc
      901 cagcggcaga gcagctcgcc gccgacgatc tgcccggtcc gcaggtcgag caccggttgg
      961 atgcacgcgt ccaatgcacc gatgcccacc gcacggcgca gctcgcgctc gaggtcgaag
     1021 cgctgctcga cagccaggcg catgtcgtcg tcgaacaccc ggatctggcc gggtcccgag
     1081 gacttggcgt ggtacatcgc cacgtccgcg tcacgcagct ggtcgagggt gtgggggtcg
     1141 cccggctcga gccacgcgac accgatcgac gccgacaccg ggatcacgtc gtcgtcgaac
     1201 gcgaccggtt ccgagagggc gtcctggatg cgctcggcga gccgggccac gacggtgacg
     1261 tcgccggcct cctcggccag cacgacgaac tcgtcgccgc cgttacgggc gaccagatcg
     1321 gtgtcccggg tggcctgcag gagccgagcg gcgacctgct ggagcacgac gtcgccgacg
     1381 cggtgaccga gggtgtcgtt caccgacttg aaccggtcga gatcgatgaa cagcagtccg
     1441 agtccctgac ctgaccgctc ggcgcgcgcc gcagcgcggt cgagctcggc gacgaggctc
     1501 gcccggttgg ccagaccggt gaggtcgtcg tgggtggcct ggtgggcgag ctgctcctcg
     1561 aggatcttgc gctcgctgat gtctcgggcg aacacggcga tgaccggatc gtcctcgtcg
     1621 ccggtggccc gggtcgtgac cgagaccgcc cgccgtgcac tggggtggtg gacgacggtg
     1681 tcggcgtcgg tgagcccgac ggggagatcg gcgagcaacg ggaaacgctc ggccaggctc
     1741 tcggcgggcc ggcccacgat ggccgacgcg gcgaggccga gcagccgggt ggcggcgtgg
     1801 ttcgccgagc cgacgatgcc gtcctcgcgg atggtgagga tggcttcgtc ggtggcttcg
     1861 accaggctgc ggctgaacgc ctcgctcgcc gcgagctggt ggtggagcgt gtcgctctcg
     1921 tgcgtcgcgg cgccgagatg ccggcggaac cggaaggcga cggccgcggc gaaggcggcc
     1981 acagcccacc ccacgatcat cagcccgacc acgcaaccag tctggtcctg ccgtgaaccc
     2041 tggtcgggga tgctgcgggc ccgcccggac ccggggttca gctgagcagc tcgccgaacc
     2101 agtcgtcgcg gtcgagcgag gagacgaagt cctggatctc ggcctcggtg atgcccccgc
     2161 cggaggggtg caggaagcgg gcgtcgctca cgccccagtc ctccaccatg cacccggcgc
     2221 cgatcagcac cgggaccatc ttgtcgctca ccggttgcac gaccgcctgc acgcagtccg
     2281 ggcagatgaa gcagcactcg gactgggtcg catcggggcg acgacggacg gtgagggcgc
     2341 cggcgtcgat cagcacggtg tcgcaggacg gacaggtggt gcgcacggtg ggcattcgag
     2401 gggtcttcct tcagcgggcg gagaccgtga cgtcggtctc cggtgtctcc cccactgtcg
     2461 caatgtccgg ttcgtcacga aatagcgagt gcgggtcatt ttccgcatag cccgtccatc
     2521 cggactacga acacatggtc cggggtcatg agggacatag actgatcttg tgtcccacct
     2581 gacgatcccc gcggccaccg accacccggc ccccggcgcc ccgctcgggc cgggctcgct
     2641 gctctggcgc atcgccgccg actggcgttc gggcctgtcc ggactcagcg ccggcatcct
     2701 gcagctggcg tggccccacc tcggtcaggg cgtcgaggag cactcggcgt tcttcgacga
     2761 gccgtgggac cgcatccacc gctcggtgcc gcgcatctgg gcgacgatct tcgccgacga
     2821 cgccgacgag cgcggtcgtg acatccgcga cctccacaag ggcatcaagg gcaccgaccc
     2881 gcacggccag cgctaccacg ccctcgatcc cgagacgttc tggtgggcgc atgccacctt
     2941 cacctgggag atgttccaga cagcggacga gtgggaccac cgcaccctct cggcccggcg
     3001 gcgcgagcag ctctaccagg agacggtcac ctggtaccgc cgctacggcg tcagcgaccg
     3061 cccggtgccc gccgaccacg ctgcgttcga agcccgcttc gacgagatct gccggaccca
     3121 gctcgagctc accccgaccg ccgagcgcgc catcgacatg gccctgcacg accgggtctc
     3181 accgcccggc atgccaggtc cggtggccac cgtcgccacg ccgctgatgc gcacgatggc
     3241 cttgggcggc ctgccccccg tggtccgggc ccgcttcggc atcccgtgga ccatggccga
     3301 cgagctggcg cttgccgggt tccgccagct cgtccgcgat gctggacggg tggtccccca
     3361 ccgcctcacc cgtggcccgt acctgcgctc gatcaagcgg atgaagcaca tcgccgcgcc
     3421 ccggcccgag cagatcccgg cctgacgtgc ccaaggtcgc gacccaggtc gacgctgtcg
     3481 ccgtgctcac cgcactggcc cagcgcccgg tcgacaccga ggccgacgac gtcgaccgtc
     3541 gcatcctcga tgccgccggt cgcctgttgg tcgacgtcgg cctcgaggag ctggaggtcg
     3601 accaggtcgc cgagctcgcc ggcgtggggc gcagcaccat ctaccgccgc ttcgacggac
     3661 gcaacgccct cctcgccgcc accgtcgccc acgaggcccg gcgtctgctc gccgcgctgg
     3721 cggatgccgt cgccggcatc gacgacccgg aggagcagct ggtcgtcgcg ttcagcaccg
     3781 gcctgcgcgt cgcccgggcc accggcctcg ccgagcgggt ccgcgacgag cccctcctgc
     3841 tccgcctgat caccgtcgac ggcggacccg tgctcgccgc ggccagcacc cagctcgccg
     3901 gcttcgccca ggcgctcgac cccacggtcc acgagcgcga cgcccgcgag accgcggagc
     3961 tgctggtccg cctcgccatc tcgctcgtgc tgacgccgga gacggcgctc gacctcgaag
     4021 agggctgcga ggagaccgta cggcgccgga tcgccccgct gctccggcgt cgctagcggg
     4081 ctgcgcccgc ggacgctcca gcgagggccg ggtcgggtgg cggcccgagc acctcgatcg
     4141 agaactgggc gcagacctcg gcgacgtggg ccgggtcgat ctcctcgggc tccgggaggc
     4201 cggtgtcgtc ggtggggcgg ccgagcgtgg cgacgaagcg ctcgaactgc gccggcgtcg
     4261 agacctgcag cacccgggcg gtgtcggagc gcacctggaa ggtgtgaggc cggccccgtg
     4321 gcagccagac caccgcgccg gtggtgtgca ccgcttcgac gtcgccgcag gcgaaccaca
     4381 tctcgccgtc gatgacgtag aagagctcgt cctccacatc gtggcggtgc agcggcggac
     4441 cgaagccacg tggtgcaagg aactccatca ccgtcatggc gccgtcggtg tgctcgccgt
     4501 cgagcttggc ggtgtagagg ttgccgagga agtggaagcg gtcgccctgg tcccgggcga
     4561 tgatggctgg gctctgtgcg gtcatggagc gaccgtacgg agaggccctc gatccgggaa
     4621 tcccgaggac gggtggacct gggtcccagc ccggggggtc ccctccccgc acctggggga
     4681 tggtgctccc ggcgactccg tcgtaccgtc ggcggcatgg ggcgggctgg catcgcgtcg
     4741 ttcacgaccg cgcgggacct cttggtcgcc gccgctgacc gggtcggcac cggcgaggag
     4801 gcctgcaccg agatcgcccg ggccttcacg cacgtcatgc gccacgacgg tgccgccgtg
     4861 atgaccaccg accccgacac ccacctcccg gcgggcggga tcgtgtccgg gttcgacagc
     4921 tcggcctgcg tgccgttctg ggacaacgag ctgctcgatc cggacttcca caagttcaac
     4981 gagctggccc gcagcacgga acgggtcgcc accctggccg acgccaccga cggcgacctg
     5041 cgccgcagcc cccggttcca gaagctgtac gagggcttcg gcgtcagcga cgagatgcgg
     5101 gtggccttcg tggcgggctc cacgtgcatg gcgatcggcg ccttcgtccg ctgcgacggc
     5161 gacgtgtact cccccaccga ggtgcgcgac gtcaagaacc tcctcgcccc ggcgacgaac
     5221 gtcttgcgac gcgcgctcgg ctgcacccgg gagccgctca ccgcgcatgg cccggtggtg
     5281 gtgctgctcg accgagacgg acagatctgc ggtcgttcgg agggcgccga cgcggtcctc
     5341 gacgacctcc ggatccgcgt ggacgacgac ctccccggca cgatcatggt cgccgcggcc
     5401 cgagcgcagg ccagccgatc gtcgacccgg ttgaccaccc ggctccgagg ccgcagcggg
     5461 cggtgggtcc gcatccacgt cgccccgatg gagggcagcg acgggacggt gtcggtcacg
     5521 atcgacgccg cccgggccgg cgacctggtg ccgatcctgc tcgactccta cggcctctcg
     5581 tcccgggaga gcgacatcgt cctgctcgtg tgccgcggcg tcggcaccaa ggagatcgcg
     5641 agcgagctgt cgatctcggt ccacaccgtg cgcgaccacc tgaaggccat cttcgacaag
     5701 gccggggtca acagccgcgg cgagctcgtc gcgcagctct tctccgacca cgtgctggcc
     5761 gacttccacc gcaccatcgc ccacgtctga cctccgcacg catcgtggtc gaaagaccgc
     5821 gctcggattt gctaggtgtg tgtgtccaac ggggacagag ccggacatcc cgaccacttg
     5881 ggacgttcga ccctgctgcc ccgggaccga cacaggaaga cttggccgag caatcgggcg
     5941 accgaccgaa ctgcgggagg cgctcgccgc ggagccgtag ggggaagcgc tcatgcaacg
     6001 tcaccgttcg accacctgga ccacgcggtc gtgggtgctc ctggccgtgc tggccatgtt
     6061 cgccactgcg tgcggcggca acgacgacga cggaggcgac gcagctccgg gcaccgaggg
     6121 cgacaacggc gcggagacga ccgagaccac ggtggaggac gccggcgatc cggtcgaggg
     6181 cggctcgatc accgtcggcc tcgaggccga gaccaacaac tggctgccgg gccagggcaa
     6241 cttctcgaac cccggcatca acgtggccct cgccatctac gacccgctca tgcggcggaa
     6301 ccccgagggt gaacccaccc cctacctggc cgagtcgctc gagccgaacg aggcgctcga
     6361 cgagtggacg ctgacgctgc gacccgacgt ggtgttccac gacgacacgc cgctgaacgc
     6421 cgaggcgatc aagacgatct tcgatgacta catcaaggcc gacgactcga acctggccgg
     6481 tcaggtcgtc gacgtggagt ctgtcgacgt cgtcgacgac ctcaccgtcg tctaccgctt
     6541 gagcggtccc aacgctgcct tcgccagcgt cctgaccggc gccgtgggct ggccgttctc
     6601 gccgaccgct gcggccgagg ccggcgcgga tgccgggtcg cgacccgtgg gcaccggacc
     6661 cttcgtcttc ggcgagtggc agcgcgacaa ccgactgacc gtcaccaaga acgagagcta
     6721 ctggcagcag gggctgccgt acctcgacca gatcgtgttc cggccgatcc ccgacgagga
     6781 cacccgcatc tccagcctcg gcaccggcga catcgacgtg ctccagagcc tgcgccagag
     6841 cgcgatcgtg cgagcccgcg acgtgcagga cgtggtcagc cacgagcacg tcggcaacaa
     6901 cagcggctcg gcgatcttca acacggccca gccgccgctc gacgacgtgc gggtccgccg
     6961 ggccctggcg tacgccctcg accaggacca gctcatcgac gtgctgggtg gcgccgggat
     7021 caccccgccg cagacccagt acttcggcga tgagagcccg ttctactccg aggccgccgc
     7081 cgaggagtgg ccgacgaacg accccgaaca ggccatgcag ctcatcgacg agtacacctc
     7141 ggacccgaac cgctccgacg gcaaggccgc cggcgacccg gtgaccttcg tgttcgactg
     7201 cccgccggac cccagcctca tcgagctgtc ccagctgtac caggcgttct gggagtccat
     7261 cggggtgcag gtcgacctga accaggtcga gcaggccacc cacatcaacg aggcactcgc
     7321 cggcgacttc gattcgaagt gctggcgggt gagcgacgag gacgatccct acatcatctt
     7381 ctccgaggcc ttcgggccgg agagtccgct gaacttcacc aacttcacgg acccggccat
     7441 caccgagcag ctcgaggtgc tgcgcacgac gaccgacatc gacgagcgca aggctgcggt
     7501 cgaggagatc tccctcgtgc tggccgagaa cgtgcccaac accttcacgg ccggcacgct
     7561 caccacgctc gccgcccgtg atgcggtgaa gaacatcccg ggctggacct tccccgacgg
     7621 cagcgagggt gagggcgtca tcggcggcac caccatgtgg ggccacgtct ggctggccga
     7681 gtagcaggac ggaccgggag caggagggaa gggaccgaac cgtgcgcgtg ctgcgcaggc
     7741 ttccaccgtt gatcggcacg ctcatcgcgg tctcgatcct gaccttcctg ctcaccgccc
     7801 tgctccccgg tgacccggcg gtccagatcc tcggggccca gagcgcgacg ccggaggccg
     7861 tcgcccaggt gcgcgccgag ctccggctcg accagcccct gccgaaccag tactggcact
     7921 ggatctccga tgcggtcacc ggcgacttcg gtcgctccta ccgcaccggc caggaggtca
     7981 gcgaggccat cgtcgagcgc ctgcccgtca ccctccagat cggtgccatg gccatcggct
     8041 tcgcgctgct gttcgccgta cccctcggga tgctgtcggc ctaccgggcc ggtggtgcgc
     8101 tcgaccgagg cgtcacggga gggacgttca ccctgctcgc catccccaac ttcatgatgg
     8161 cgatcctgct catcctcatc ttcgcggaga tcctcggctg gctccccgcc accgggtggg
     8221 tccgcctcag cgacgacccg gccgccaacc tccgcgccgc gctgctgccg gcgctctcga
     8281 tcaccatggc tgagctggcc gtctacacgc gcctgctgcg cagcgacatg atctccaccc
     8341 tgcaggagga ctacatcgcc gtgtcgcggg ccaagggcat gccgacgtgg tggatcctgc
     8401 tgcgccacgc cctgcgcccg tcgtcgttct cgctgctcac cgtggtgggc gtccagatcg
     8461 gggccatcat cagcggttcg gtcatcatcg agacgttgtt cgccctgccc ggtgtcgggc
     8521 gcctcctcgt cgattcgatc ttccagcgcg acctgatcat ggtccagggc atcgccctgg
     8581 tggtggccgt gtcctacgtc atcgtgaact tcatcgtcga catcttgtac tcgtacctcg
     8641 atccgaggat ccgccgtggc tgagcctccc gcccacgaac ccgagcacac cgagctcggc
     8701 gccctcgccg gccaggcggg cgagcccggt gaggacggga tggggaccat caccgtcgtc
     8761 gacaccgcga ccgcgtcggt gcccgccgcc cccgcccgca aggagggcaa gggcttcgcg
     8821 ttctggcttg ccgtcgcatg gctcgtcgtc gtggcgatct gcgccgtgtt cgccgagcag
     8881 cttccggtcg ccgacgaggt ccgccccaac gtcatcgaca agctggcgac gccgagcgcc
     8941 gactatccgc tcggcaccga cgggctcggt cgcgacacgc tcgcccggct cgtccacggc
     9001 gcccgggtgt cggtcgtggt gagcctcagc gccgtcgggg tcggcatgtt cgtcggcggc
     9061 accctcggca tgatcgccgg ctacgtccgg ggttggttcg agacgctgct gatggccgcg
     9121 gtcaacgtga tcctcgcctt ccccggtctc gtgctgctgc tggtgctgct cgcctacgtc
     9181 ggccagagcc tcggggtgat ctcgctcgtg atcggcgtgc tgtccatccc catctacacg
     9241 cgggtcgcgc gcgccaacac gctcgccgtc gcccagcgcg agttcgtcct cgccgcccac
     9301 accatgggtg cgaaccggct gcgcatcctg ttccgggaga tcgcgcccaa cgtgatcatg
     9361 ccggtgctgg ccttcggcct cgtcgccatg ggcgtgatca tcgtgctgga gggggcgctg
     9421 gcgttcctcg ggctcagcgt cgaggctccc acgccgacct ggggcggcat gatcgccgag
     9481 ggcaagcgcc acctcagcac cgacccgcac gtggcgctga tcccgtcggc tgccatgttg
     9541 ctcaccgtgc tctcgctcaa cttcgtcggc gacgtgctcc ggcgcggcta cgacgtccgg
     9601 gaggcgaacc tgtgatcttc cgcaagggcg gccgggacaa gtccttcgaa ccggtcgagg
     9661 gcgcgccgct gctcgaggtg cgggacctgc acacccgctt ccagaccccc cggggcatgc
     9721 tccacgccgt cgacggggtg tcgttcaccc tcgagcgcgg ccggaccctc ggcatcgtcg
     9781 gcgagtccgg ctcgggcaag acggtgctgg cccgctcggt catgaacctg gtgttctccc
     9841 gcaagatgga gcgggaggga tcggtcctgt accggggccg ggacctgcac gagatgtcca
     9901 tgcgggagat gtacgacgtc tggggactcg agatcgcctc ggtcttccag gacccgatga
     9961 cgtcgttgaa ccccacgctg cgcatcggcg accagctcgc cgagtcgctc tggtaccacc
    10021 tcgacatgcg gggctccgag ctgcacgaga tgcaggtcgc cctcctgcga tcggtgggga
    10081 tcccggaacc cgagcggcgt ctcgacgtct acccccacga gctctccggc ggtctgcgcc
    10141 agcgcgtcac catcgccatc gccctcgcct gcggacccca cctgctggtg gccgacgagc
    10201 ccaccaccgc gctcgacgtc accgtgcaga agcagatcct cgacctgctc gaccgccagc
    10261 agaaggagcg ctacatgggg atgatcctca tcacccatga cctcggggtc gtggccgggc
    10321 gcgccgacga gatcgccgtc atgtactcgg gccagatcgt cgagctgggc accacccggg
    10381 cgctgttcca cgagcggcgc cacccctaca ccgatggcct gctgcgctcg atcccgaggc
    10441 tgtcgaacgt gccccacagc aagctgtcgg tgatccccgg gcgccccccg gacccggtga
    10501 accgcccggc ggggtgccgg ttcgcgcccc gctgcccgca cgcccagccc cgctgcctcg
    10561 agcaggtgcc ggagctgcgc agcgacgagc agggtgacga tcccaaccac cagttcgcct
    10621 gcttctaccc ggtcggcacg cccgagggcg acgacgccca cgccaccaac ctggcgcggg
    10681 gcaccaacgc cgccggcacc ccggtgggcg acgagacgga ggtcccagcc tgatggccgc
    10741 cggcggcacc gagaccccgc agctgcgcga ccgcgacgac atcgtgctct ccgtcgagca
    10801 cctcgtcgtg gagttcccca ccccgaacgg caccgtccac gccgtgtccg acgtcagctt
    10861 cgacgtggcc gccggcgaga cgctcggcct ggtcggcgag tccggctgcg gcaagtccac
    10921 caccgcccgg gcgatcatgc aggtcccccc gcccacgtcc ggccacatct tctacgaggg
    10981 caccgacctc ggctcgctcg accgcaagga gctgcgccgc acccgcccga agctccagat
    11041 gatctaccag gatccggtct cgtcgctgaa cccgcggcgc aaggtccggg ccatcgtggg
    11101 cgagggcctc tcgatctggc gacgggaccg cgccgacgag atcgtcgagg aggtcatgcg
    11161 ggcggtgggc atcgaccccg agctcgccgg cgaccgtcac ccccatgagt tctccggtgg
    11221 tcagtgccag cgcatctgca tcgcccgctc gctcgccctg gaccccagcg tgctcatctg
    11281 cgacgagccc gtctccgccc tcgacgtgtc ggtccaggcc cagatcctca acctcctcga
    11341 ggagatgaag cgccgctacg agctgacgat gctcttcgtg gcccacgacc tggccgtggt
    11401 gaagaacatc agcgaccggg tggccgtcat gtacctgggc aagctgtgcg agatcggccc
    11461 gtccgtcgac gtctacgagc gcccggccca cccctacacg cgtgccctca tggccgcggt
    11521 gcccgagccc gatcccgacg tcgtgctcga tgaggacgag ggcctggccg gcgagctccc
    11581 ctcccccatc tccccgccga gcggctgccg gttccggacc cgctgcccgc tggccgagga
    11641 ccgctgcgcc gaggaggccc ccgagatgcg cgaggtcggc ccgggtcacc acgtggcctg
    11701 ccacttcccg ctggtcgagc cggccgagac ctgatcggca ccgccgccgc cacgaacccg
    11761 gcatagcggc cttcgcggac gaaccggccg caagtcattt cccgaccgca tctgcgctgc
    11821 tagcgtgcgc ggcgacaacg gggcggctcg ggggtgcggt gcacacgacg gagcacggtg
    11881 ttcgactgca gtacggcggt cgggcgcgac aagcgcacga cgtcgtcctg gtcgtggacg
    11941 acgaggacgc gatgcgcgcc gcgttcgagg ccgcgctgcg cgacgacgga ttcaccgtgt
    12001 tcagcgcgtc gaccggggcc gaggccctcg tcctcgccgt cgaggtcgag ccgaccgtcg
    12061 tcctgctcga cgccaacctc cccgacatgc ggggcgagga cgtcctcgtc gagctgcggc
    12121 ggagcaacgg atccgagcag ccggcggtcc tgatcgtcag cggggaccgg cagctcgacc
    12181 gcaaggtcga gggactgcgc ctcggcgccg acgactacat caccaagccg gtggcccttg
    12241 ccgagctggt cgcccgcgtc cgccgccacg ccggatcccg ccaccgctgg ctcgcacagc
    12301 tcgacgacgt cctcgacgac cgccggcgac tcgcccagcg catcgccggc ctcgacagca
    12361 gcgcgccgct ccacctgctc gccgccgagc tgttcggcat cttcgccgag cagctcacgt
    12421 gtgacgcgct cagcctcacc agcgcgctcg acgggtccgt gcagcacacc gtgctcgccc
    12481 cgtccgccac ccggttccgc gccgcgctcg agcgcggtgc gggtgcaggg gcgcgccggc
    12541 cgatgatcga ccgtggcgag cagggctgga cctgccacgc accgctggag atcaccggga
    12601 gcgccttcgg cgtgctcggc gtcgccggca cgggcgacga gcagggcacg ctgtcggcgg
    12661 tggtcgacct ggcgccgcag atcgccggcc tggtgcaccg cgccctcacg tccgacaccg
    12721 ccgtcgccgg gcaccgtcgc caggtcgagg cggtcctctc cgacgacgcg ttctgggccg
    12781 agttccagcc gatcgtcgac ctccgatccg gctccaccgt cggctacgag gcgctgaccc
    12841 ggttcccgga cggacgacga cccgacctgt ggttccgcca ggcggccggg gtcggcctcg
    12901 gcgccgatct ggagctcgcc gccatcgagc gcgtcctcga cacctcgcac ctcctccccg
    12961 gggacacgtg gctgtccatc aacgtgtcgg cccacacgct catcgagcac gacctgcgac
    13021 cggtgctggc gcgcgcagac cgtcgcgtga tcctggagat caccgagcac gagcgggtgc
    13081 gcgactaccg agccatcacc cggagcatgc agggcctcgg cgacgccggc ctcggggtgg
    13141 acgacgccgg ttcgggctac gccagcctgc gccacatctt cgagctgcag ccggagctgg
    13201 tgaagctgga tcgcacgtgg atccacggca tgcaccagga cccggtccgc actgcgctga
    13261 tcaccggatt gctccacttc gccaccgagc tcgacgcatc gctgctgggt gagggcatcg
    13321 agcacgccga ggaccgggcg gcactcgagc gcaccggcgt cacgctcggc cagggctacc
    13381 tcttcggtcg gccgcgaccg gcggagatgt ggacgtcctg atcggcgtcg tgccgcccta
    13441 cgaggcgggg tcgcccagga cctcggcgag cacctcgagc agccggcgcg tctcgtaggg
    13501 cttgggcagg aagtgccgca ccccggccgc tgacgcctgc gccaccttcc cgttcccgtg
    13561 cagcccgctg gctccgatga cccggacatc cggatcgagg ttctgcaggg cgtggatggt
    13621 cgctggtccg tccaacaccg gcatcatcat gtcggtgagc accacgtcca cgccgccggc
    13681 ctcccgagcg aagacggcca cggcctcggc accgttggcg gcctcgagga cccggtagcc
    13741 gtaggtctcc aacgtctggc gggtcatggc acggatggca gcctcgtcct cgaccacgag
    13801 cacgctctgc ccggaccctc ggggcgggtc cgtgtccgcc cgtggcgtcg caggttgctc
    13861 ctgcggcgcg aggtcgcacg ggaggtacac ccggaagcgg gttccccggt cgggctcgct
    13921 gtacacctgg aggaacccgt cgtggctctc gatgatcgcc accgcggtgg acagtccgag
    13981 accggtgccg acgccgtgcg ccttggtcgt gaagaacggc tcgaaggcac ggtcccggac
    14041 ctcgggagcc atgccctctc cgtcgtcctc cacctcgacc agcaggtagc tgcccgcctt
    14101 ggcgccgggc gccgtcgcga cgtactcgtc gtcgatcacc acctctgagg cggccagccg
    14161 caggtgcccc ccgtccggca tcgcgtcccg ggcgttcacc acgaggttga gcaggacctg
    14221 ctggatctgg gtgctgtcgc ccacgatcgg ggccagggac gcggccacgt ccaacgtgag
    14281 cgagatgttc ttggggaagg tgttccggac gatggccccg atgtcggtga ccagcgaccc
    14341 cacctcgacc ggtcggcgct cgccctcgac gccgcgagcg aacgacagca cctgcgacac
    14401 gaggtctgcg cctcgtcggg cgctcgtcac gatggtgtcg accagggtgg accgatcgat
    14461 gttgaccgag tcggactgca ggagctcgcc ggcgagcagg atcggtgcga gcacattgtt
    14521 gaggtcgtgg gccacgcctc cggccagggt cccgatgctc tccatccgct gcgcccgcag
    14581 gagctgctgc tccacccgct tgcgctcggt gatgtcggtg ttgatggcga ggacggcctg
    14641 cgggctgccg ctcgggttcc ggacgagggt ccaccgggcc gcgacctcga cgaggtcgcc
    14701 tcgtcgcgtc cgctgccgga gctcgcctga ccaccggccg gttcgcagca cggcctcgtg
    14761 ggcagcatcg aaggcaccac ggtcctcgta cagcagggag cgcacgtcgg agcccagcgc
    14821 ctcctcggcg aaccagccgt agatgcgctc ggcgctgcgg ttccagtacg tgatccgacc
    14881 ccccggatct cgcacgatga tggcgtcctg ggcctggtcg agcaggtcgg cctggcgctg
    14941 gagcacctgc tcgcgctgct cgagcgcgac ccgagccgcc cgggcgtcgg tgatgtcccg
    15001 gaagtagacg gcgaggccct ggtctgccgg gtacacgtcc acctggaacc aggtgtccag
    15061 cggcgggaag tggaactcgt ccacgtggcg tgtgcgccgg tcggccatcg acgcggtgta
    15121 cacctcgtgc acctccgttt cggccgcctg ggggaactcc tcccacagac cgcgaccgag
    15181 gaggtcgtct gcgtcgcgtc gcaagagctg ctcggcgttg ccgttcaggt aggtgaagcg
    15241 ccagtcccgg tcgaccatga acacggcgtc ggtcatgctc tcgagcgtgg accccaaccg
    15301 ccgggctgcg tcctgggcct cctgggtcgc catgcgcagg tcggagatgt cctggaaggc
    15361 acccatcacg ccgcacacct ccccctcgct gtcccggtcg ggctcaccga tgatgcgcac
    15421 cgggatccga cggccggcga aggtgtcgat ctcgagctcc acatcgaagg gggtgccgtc
    15481 gcgcaggcag cgctcgagcg cctcgctgat cgtctcgcga tgggcatccg gatagcgctc
    15541 gatggcgtcg tcgagtgcgg gaacaccttc gtgcgacggg gactcgagga tgtcgtggag
    15601 ctcgtcagac cagtagacgg tgcggtcagc gccgtcgatc cgccatcccc cgacccgcgc
    15661 cagtcgtccg gcgagccggc gcagcgcctc gctctgctcc aggcggagcg cggcgctgcg
    15721 ctcctcgtcg tccgatggcc gtgtcaacgg ccggccccca gcagcaccac gggctcatct
    15781 tgccccagcg acgaccgatc ccacccgatg ccaggcattc gacgcggtga taccgttcgc
    15841 cgcgacctcg ggggggcgtc atgttgacag gtgcatcgaa gctcgagacg acgcagatgc
    15901 tggccagcct gccgttgttc gccggcctga cgaagcggca gctcggcgcc gtctccaagg
    15961 cggtggacca cgccagcttc gagccgggga ccgagctcct cacggagctg aaggaggtcc
    16021 aacgcctcat catcatccgc agcggcaccg cagcgatcac ccgtcgcggt gccgtcgccg
    16081 gcgatggcgg cggcctgcag gccgggacca gccgccggct cggcacggtc ggccccggtg
    16141 acgtcgtcgg cgagctgtcg ctcatcgacg gccacgtcgc gtcggcatca gtggtcgccg
    16201 agaccccgat cgacgctgtg ctgctctacc ggaccaggtt caacagcctt ctggccgcca
    16261 cgcccaagct gtacccgcgc ctgctcgtcg gcatggccac tcggatccga gcgatggacc
    16321 ggcacaccga catgatcagc tgacgccgtc cggcggcgtc ccccaaccgc cgccgcctgg
    16381 cgtcagcatc cgcagcacgt caccggcctg caagcggatc gtgcacttgt cggggagccg
    16441 ctcgacgcgc gcctcgtccc cctgcgggag cagccagttc tcccccgtcg caccgggcgc
    16501 accgccggcc agtccgttgg gacgggacac gcggcgctcg gtgatcagcg acacggtgac
    16561 gtcctcgagg acctgcagat cgcgctcgat gccctcgccg cccggcgcca ggccggcacc
    16621 gccgctcccc cgccggagtc ggtagcgcag gacccgcaag gggaagtggc gctccaacgc
    16681 ctcgatcggc gtgttcttcg tgttggtcat gccggtctgg atgcccgact ggcccggcgc
    16741 cggggaccct tccgccatgc gaccgccctg accgccgccg acggtctcgt agtacaccca
    16801 gccccggccg ccgatgagca ggttgttcat cgtgccctgg ttcccggcgg ccacccgctc
    16861 gggatccgat gcggccaggg cggcgagcac cacgtcggcc actcgctggg acacctcgac
    16921 gttcccc
//
LOCUS       DATISU010000140        14356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_845064, whole genome shotgun sequence.
ACCESSION   DATISU010000140 DATISU010000000
VERSION     DATISU010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 14356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14356
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845064"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VLR27_05820"
     CDS             complement(<1..589)
                     /locus_tag="VLR27_05820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP02998.1"
                     /translation="MNFAFSEEQEELRNIVRSFLENKSSEETVRELMETEKGFDDAVW
                     SQMAEQMGLQSLIIPEEYGGSGYSYVELCVVLEEMGRRLLAAPYFSTVVLGANTILQS
                     GDDAAKKDLLPGIASGETKATLALTESNGRWDESGIEATATKSGDGYAISGEKMFVLD
                     GHTADLIIVAAKSDAGTSLFTVKSDASGLTREALST"
     gene            694..975
                     /locus_tag="VLR27_05825"
     CDS             694..975
                     /locus_tag="VLR27_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HSP02999.1"
                     /translation="MESIGVRELRNNVAAVLRRAAAGERIVVTVDGVPSAQLGPIEPT
                     TNPTLEDLVATGLVQPPQRPGTGPTEPAPIDVAVDIRIDDVLTELRGRE"
     gene            972..1391
                     /locus_tag="VLR27_05830"
     CDS             972..1391
                     /locus_tag="VLR27_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSP03000.1"
                     /translation="MTLLAIDTSALLRRYVRTSGHGLVTEAMAESDTWCASALARTET
                     LQAMHAVAVTPGQHDALWSALRADWERFHVVPVDDRCLARAVDIGATFRVRSTDAIHL
                     AAAERLPAPVRYLTFDRRQIPAAAGLGFDVIAPREDL"
     gene            1411..2019
                     /locus_tag="VLR27_05835"
     CDS             1411..2019
                     /locus_tag="VLR27_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSP03001.1"
                     /translation="MSTLHWEDTAAEIHKVVVGPMDNNVFVLRCKQTGDALLIDAANE
                     HERLLELCQHLGVRTVIETHGHWDHIQAVPELRDAGYSVHVTAEDAAMLPAYDEILED
                     ESVIEVGRLRLHTIHSPGHTPGSMSFRLDGAPILFSGDTLFPGGPGNTSFEGGDFDTI
                     IASLDQRLFTLPPDTLVLPGHGDDTTIGTERPHLQEWIDRGW"
     gene            2025..2411
                     /locus_tag="VLR27_05840"
     CDS             2025..2411
                     /locus_tag="VLR27_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03002.1"
                     /translation="MSDALPYDERPTDDTPVHTADLPPTPIRDRNIPATAWVEAPTSL
                     TSLGDDLPDQPVAEYKRRIGPWILWRAGPASKGHARYWAAHEDDLDRAYTLRLFPDGS
                     AEGTGPSGTVHERFRAWKEDLRDSED"
     gene            2461..2646
                     /gene="tatA"
                     /locus_tag="VLR27_05845"
     CDS             2461..2646
                     /gene="tatA"
                     /locus_tag="VLR27_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HSP03003.1"
                     /translation="MPQGPELIIILIVILLLFGGAKLPKLARSLGQAQNEFKRGLKDG
                     SENHDADDTTTERTKDA"
     gene            2712..3497
                     /gene="sufC"
                     /locus_tag="VLR27_05850"
     CDS             2712..3497
                     /gene="sufC"
                     /locus_tag="VLR27_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HSP03004.1"
                     /translation="MAASTTPLFEIEDLHARVAEAPAGEAAAEILKGIDLTVHPGEIH
                     AIMGPNGSGKSTLANTLLGNPEFEVTAGTIRLRGDDITDWDVDVRGKAGMFLGFQYPQ
                     SIAGVSVINFLRQALSARKGIDLSVLELRLSIMEWMERLDMDPSFADRYLNEGFSGGE
                     KKRNEILQMAILEPEIAILDETDSGLDIDALQVVAKGVHEVRAARPDLGVVLITHYQR
                     LLEHLRPDHVHVLVDGRIVASGGMELAHQLEADGYEAFRGVDA"
     gene            3494..4741
                     /locus_tag="VLR27_05855"
     CDS             3494..4741
                     /locus_tag="VLR27_05855"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HSP03005.1"
                     /translation="MSEAGTTLDVARIRKDFPILSRDVRGTRLVYLDSANTSQKPQSV
                     LDAMDHYYERSNANVHRGTYLIAQEATDALEGARGIVRRFIGAPSDRTVVFTKNATEA
                     LNLVVRAWGTKHLRAGDAVVLTEMEHHANIVPWHQLAEAIGIELRWIPVTDEGHLDVS
                     DLDRLLDGAKVLSFTAMSNVLGTVTPVAELVAKAHDAGAIAIIDASQHVPHITTDVQA
                     WGADFVAFTGHKLLGPTGIGVLYGTEEILEATPPFLGGGEMILNVTKDGFTHTELPWK
                     FEAGTPMIAEAVGLGAACEYLTGLGMDAVRQHEVELTAYTLRTLTERFGDDVTIHGPS
                     EPAARGGVFSFAYKDIHPHDLTQVFDEHGVCVRAGHHCAKPLMRALGVNATARASLYV
                     YNDTDDIDALADAMDAAGQFFSF"
     gene            4751..5263
                     /locus_tag="VLR27_05860"
     CDS             4751..5263
                     /locus_tag="VLR27_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HSP03006.1"
                     /translation="MPGLEDLYREIILDHYRSPRNKGELEAPPATRVEGFNPLCGDEI
                     VVYVDVEDDTVKDIRIGGQGCSISQSSASMMSAAVKGKTLEEVKGLTKAFKAMMSIHE
                     QSLDGEGEGLEESGESGIDPNEIADVPEEVKLGDLEALRGVVKFPVRIKCATLSWNTL
                     AQGIDEATAG"
     gene            complement(5271..7352)
                     /locus_tag="VLR27_05865"
     CDS             complement(5271..7352)
                     /locus_tag="VLR27_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSP03007.1"
                     /translation="MTSGREASAQAGPDDAVGAPARRARFQPIVDLRTGRPIGWEARP
                     HRWVDDAPDGGLAVLASLEAAGSPPDDGLVFVHVSADLLGDPRLYEECERLGSRVVVD
                     VRGAEVDRLVELSSDLDALATAGIHLSIENTSGVSLALIARVRPTFVKLDPPIVRDIV
                     HDPANRSVVRAYVAFAEHEGIHVIAEGVERHDQLDELRALGVVLARGPLFGQPTGDWA
                     PPSPRRRRPPLVLDTVRQAKQSIGAAGDANAVAEQLVAELERIGDRSAVYFERDGLLR
                     CVAEHGHAEVLDGIPVRSGLLGSAFRARGLLRGDDEHGEGVRLAHPIHVADRAVGVVT
                     AVGESGFTTEQESHLIELVGIAGARIGEVGSPRAETAMHLLARATNELALLTDVHQVE
                     IAAARLAGVVGGMSSTLFALPGTTGLDIKSLQGPLAPELRQLSPETLRSLLDHVGSAS
                     SCFVAAGRSGMRPELVKLCEAGASSVVALPVRSGERGVGLLLLADHAPMALGLDCREA
                     LELLASELGRALDMVVLVDDLRTRATVDTLTGLGNLSAFQEALSNMGARRRGGWALAM
                     ADVDGLKLINDTHGHLTGDFALRSLAAVLHDVLRSEDRMFRIGGDEFAALLQDVDADG
                     AADIGRRMCEAAASVMADFGAGLSVGIATPEQGETPAEFIDRADKMLYEVKRSERGTV
                     RVAPPTERPTD"
     gene            complement(7548..8270)
                     /gene="sufC"
                     /locus_tag="VLR27_05870"
     CDS             complement(7548..8270)
                     /gene="sufC"
                     /locus_tag="VLR27_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HSP03008.1"
                     /translation="MSTLEIKGLTADVAGKEILKGVDLTVSSGEGHAVMGPNGAGKST
                     LSGVLAGHPSFTVTGGTATLDGVDLLAMPAWERAKAGLFLAMQYPVEVPGVRLEEMLV
                     ESAVAAGRDPGSVRDTIAAEAANIGFEARFLDRPLNVDLSGGEKKRNETVQLATVRPR
                     IAILDEIDSGLDIDALRDVSRRIEQLTEEGLGVLAITHYRRLFTELRPDVVHVLVAGR
                     IERSGGPEIAEELEASGYGAEG"
     gene            complement(8267..8581)
                     /locus_tag="VLR27_05875"
     CDS             complement(8267..8581)
                     /locus_tag="VLR27_05875"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HSP03009.1"
                     /translation="MSTRICSLDDVGPDSAIRVDIDGHRLAIVRCGDEVYVIGDRCTH
                     ADISLSEGEVDCEDGTIECWKHGSTFRLDDGVPTVLPATKPTPTYEVIVKDGDVYVEL
                     DA"
     gene            complement(8578..9840)
                     /locus_tag="VLR27_05880"
     CDS             complement(8578..9840)
                     /locus_tag="VLR27_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HSP03010.1"
                     /translation="MTSTFSPDAAAALPGPQWLVDRRRAAAERFAGAELPSAEEEVWR
                     YSRIGDLALDRYRPSTADDATGADHGRADMLLELTAGASAVVWTVAGQVTRVDLADGA
                     KVQVTTGDDLAADGPLGSVLGDTTDSLSALHDAFAAPIVVQVPAGADAGQVVVVHTSG
                     PEGVASFPRLVVELGQASSASVVEVHHGPDGVQSLALPVTELAVGRDATLRHRTLQLL
                     GDGAWQIGRLASTVDQGGTLHAGTAAFGGDYARTRIDTTLAGRGAHGEITAAYFGDGS
                     QLLDFRTFQDHVAPDTTSNLLFKGALGEKGASVYTGLIKIRPTARGTQAFQTNRNIKL
                     SDDAWAESVPNLEIENNEVQCSHASTVGPVDAEQRFYLESRGVPTGRAERLIVRGFFD
                     EVLDGLALGSLEDVVRDSLEAKLERTMP"
     gene            complement(9967..10335)
                     /locus_tag="VLR27_05885"
     CDS             complement(9967..10335)
                     /locus_tag="VLR27_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03011.1"
                     /translation="MPMRQDCKYFESRTYPNGDTVRKCDLDLAPHAPWRCPEDCPKYA
                     PRLVDVNWQHGTLVTPETPVEPPGLDEDDGSIAALLDAAEDIVNAVGAEMVADVERER
                     RAAESPRWKRLFKRSRKKDS"
     gene            10417..11730
                     /locus_tag="VLR27_05890"
     CDS             10417..11730
                     /locus_tag="VLR27_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP03012.1"
                     /translation="MDPGQDADSDVAPEATGWGALGRNHKLLLALLGATSFFDGFDRG
                     ILTVALPQIRDTYDLDQGQMSLWISLLYVGALPALLLTRRADKVGRRRLLLISIVGYT
                     IATGLTAVAPSIEWFVAFQFIARLFLNAEHALVFTLAAEELPAKARGFGFGFLSMNLA
                     LGVGLSAIVFGTVFEPAGISWRAMYLVGLPPLVLIGWLRRRIPESRRFEAARDGGELT
                     GSWRAILAPGHRRWLLLLCATAFLLELGTHASVFTQDFLQEDKGLSATLASLMLVAAG
                     LPGIPIMVWAGGLSDRLGRRLVGCSLAAVGVVGALGFFWLPGGVPVLLAFMALTLVGQ
                     MGAGPVLGTYATELFPTALRGQAGSWAKVAAVGGQAGSLALGGVLISLTGGLPGAATV
                     LMIGPIVAIAVFAVSFPDTHGRELEDTAGGYPDVDGVAAELATPI"
     gene            11756..14005
                     /locus_tag="VLR27_05895"
     CDS             11756..14005
                     /locus_tag="VLR27_05895"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HSP03013.1"
                     /translation="MTEPTRIIRDVDGIAHVRAASVLAAFEGQGFAASEDRIWQMELD
                     RRRALGTLAEVVGPSAVAVDTFHRRMGLATHARASLDALDDETRAVLDAYAAGVNRGL
                     DSDADLPPELVALGVVLAPWEAWHSIALYEVRHLAMGTYETKLWRSGLVHRLGAEAVA
                     RLWPTTDEVLVDPTAPDAPVPIDVAAALPAGEGLLSRFSLADVGGTGEHGSNNLVIGP
                     SLTATGRPILAGDPHRAIDLPNVYWQNHLTCTDPDDPLDVIGLSFPGVPGFPHFGHNA
                     DVAWCITHGMADDQDLSVVDLRRTGDGVEHHRADGWHAADVRTEEVAVAGGDGIEVEC
                     VTTPAGPVVASLERDGTWTGIALRWTATAAVDTTAAALLPMVRACSVDDLDRAFEPWV
                     VPVNNVLMADTAGAIAYRMRGRVAVRAASSGWTVVDGADPATAWEGFVPDADLPRWRD
                     PDRGFLVTSNNRIAADGPYVSHDWAHPTRARRITELLAGRDGWSVDDVGALLGDTHSG
                     VAAVLARRLGALSPSHPMEVRALALLDGWDHHMDVASSAAAVVGTVRGELLGLLVHEL
                     GLADDRLPGVSGPSLHQTMRFANARLAWWIDDPGLVRDRTLSSALRLAVRQLELTQGP
                     DPSRWRWGAAHLARWAHPLLALRPDLADQVVVPPTVELGGDNECVWATSTAPPSTEAS
                     NGQVARYVFDVGDWDRSRWIVPHGVSGDSRSAHHLDQLEDWAGMRLRPMRWSADVVDA
                     ATESVTALP"
     gene            complement(14073..>14356)
                     /locus_tag="VLR27_05900"
     CDS             complement(14073..>14356)
                     /locus_tag="VLR27_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03014.1"
                     /translation="VLPGGSWTGTVRLGQDLFGNWCDDVLEPGEPTPEVLEEWPVVEG
                     SIEVVLPPDGAWGPATLVATGLVAERPDGARVVIGDITIENSNHGLIPG"
BASE COUNT         1992 a         5265 c         5050 g         2049 t
ORIGIN      
        1 tggtggacag cgcctcgcgg gtgaggcccg aggcgtcgga cttcacggtg aacagggagg
       61 tgccggcgtc ggacttggcg gcgacgatga tcaggtcggc ggtgtggccg tcgagcacga
      121 acatcttctc gcccgagatc gcgtagccgt cgcccgactt ggtggcggtg gcctcgatgc
      181 cggactcgtc ccagcggccg ttggactcgg tgagtgcgag ggtggccttg gtctcgccgg
      241 aggcgatgcc ggggagcagg tccttcttgg cggcgtcgtc gccgctctgg aggatggtgt
      301 tggcgccgag gacgacggtg gagaagtagg gggcggccag caggcggcga cccatctcct
      361 cgagcaccac gcacagctcg acgtagctgt agcccgagcc gccgtactcc tcggggatga
      421 tcaggctctg gaggcccatc tgctcggcca tctggctcca gacggcgtcg tcgaagccct
      481 tctccgtctc catcagctca cggacggtct cctcggacga cttgttctcg aggaacgagc
      541 gcacgatgtt gcgcagctct tcctgctctt cggagaaggc gaagttcacg ttggggtctc
      601 cctgtcggcg gttccttgac tggccggtca aggtaggcgc acccgacgcc gccctcaact
      661 cgtcgtgtgt acaccggtgc tacggtcgcc atcgtggagt cgatcggcgt gcgggagctg
      721 cgcaacaacg tcgccgccgt gctccggcgg gccgccgccg gcgagcggat cgtcgtcacc
      781 gtcgacgggg tcccgtccgc ccagctcggc ccgatcgagc ccacgaccaa ccccaccctc
      841 gaggacctgg tggcgaccgg cctcgtccag ccgccgcagc gtcccggcac cgggcccacc
      901 gagccagcac ccatcgacgt cgccgtcgac atccgcatcg acgacgtcct caccgagctg
      961 cgggggcgcg agtgaccctg ctcgccatcg acacctcggc cctgctccgc cggtacgtgc
     1021 gcaccagcgg ccacggtctg gtcaccgagg ccatggccga gtccgacacg tggtgcgcct
     1081 cggcgctggc tcgcacggag accctgcagg cgatgcacgc cgtcgccgtc accccgggcc
     1141 agcacgacgc gctctggtcc gccctgcgcg ccgactggga gcggttccac gtcgtaccgg
     1201 tggacgaccg ctgcctggcc cgggcggtgg acatcggggc gacgttccgg gtccgctcga
     1261 ccgacgccat ccacctcgcc gcggccgagc gcctgcctgc ccccgtccgc tacctcacct
     1321 tcgatcgccg ccagatcccg gcggcggccg gcctggggtt cgacgtcatc gccccacgcg
     1381 aggacctgtg agccgactta ggctgagcgc atgagcacgc tgcactggga ggacacggct
     1441 gccgagatcc acaaggtggt ggtcgggccg atggacaaca acgtgttcgt cctgcgctgc
     1501 aagcagaccg gcgacgccct gctcatcgac gcggcgaacg agcacgagcg cctgctggag
     1561 ctgtgccagc acctcggggt gcgcacggtc atcgagaccc acggccactg ggaccacatc
     1621 caggccgtgc ccgagctgcg cgacgccggg tacagcgtcc acgtcaccgc ggaggacgcc
     1681 gccatgctcc cggcctacga cgagatcctc gaggacgagt cggtcatcga ggtcggccgc
     1741 ctgcgcctcc acaccatcca ctcccccggg cacacgccgg gttcgatgag cttccgcctc
     1801 gacggggcgc cgatcctctt ctccggcgac accctcttcc ccggtggccc cggcaacacc
     1861 agcttcgagg gcggcgactt cgacaccatc atcgcttcgc tcgaccagcg cctcttcacc
     1921 ctcccgccgg acacgctggt gctcccgggc catggtgacg acaccacgat cgggacggaa
     1981 cgaccgcacc tccaggagtg gatcgaccgg ggatggtgag cccggtgtcc gacgcactcc
     2041 cctacgacga gcgccccacc gacgacacgc cggtccacac ggccgatctc ccgccgaccc
     2101 ccatccgcga ccggaacatc ccggccacgg cctgggtgga ggcaccgacg agcctcacct
     2161 ccctcggcga cgacctcccc gaccagccgg tggccgagta caagcggcgc atcggcccgt
     2221 ggatcctgtg gcgggccggt ccggcctcca aggggcacgc ccgctactgg gccgcccacg
     2281 aggacgacct cgaccgcgcc tacacgctgc ggctgttccc ggacggctcc gccgagggca
     2341 ccgggccgag cggcacggtg cacgagcgct tccgcgcctg gaaggaagac ctgcgcgaca
     2401 gcgaggactg aggcccgcgc cagaacggtg cggcgggcac tgcggtaccg tggcggtggt
     2461 atgcctcagg gaccagagct catcatcatc ctcatcgtga tcctgttgct gttcggcggc
     2521 gccaagctgc ccaagctggc ccgttccctc ggccaggccc agaacgagtt caagcgcggg
     2581 ctgaaggacg gctccgagaa ccacgacgcc gacgacacga cgaccgagcg caccaaggac
     2641 gcctgaacgg cgcaccggac gctcgtcaca cccattatca ctacggccgt agtggtaata
     2701 cactgggcgg catggctgcg tccaccaccc cgctgttcga gatcgaggac ctgcacgcgc
     2761 gggtcgcgga ggctccggcg ggcgaggcgg cagccgagat cctgaagggc atcgacctca
     2821 ccgtccaccc cggcgagatc cacgccatca tgggcccgaa cggctccggc aagtcgaccc
     2881 tcgccaacac cctcctcggg aaccccgagt tcgaggtcac cgccggcacg atccggctgc
     2941 ggggcgacga catcaccgac tgggacgtcg acgtgcgggg caaggccggc atgttcctcg
     3001 ggttccagta cccccagtcg atcgccggcg tgtcggtcat caacttcctg cgccaggcgc
     3061 tgtcggcacg caagggcatc gacctgtcgg tcctcgagct gcgcctgtcg atcatggagt
     3121 ggatggagcg cctcgacatg gacccgtcct tcgccgaccg ctacctcaac gagggcttct
     3181 ccggcggcga gaagaagcgc aacgagatcc tgcagatggc catcctcgaa cccgagatcg
     3241 ccatcctcga cgagaccgac tccggcctcg acatcgacgc cctgcaggtg gtggccaagg
     3301 gcgtgcacga ggtgcgcgcc gcccgccccg acctcggcgt cgtcctcatc acccactacc
     3361 agcgcctgct cgagcacctg cgccccgacc acgtccacgt gttggtcgac ggccgcatcg
     3421 tcgcctccgg gggcatggag ctggcccacc agctcgaggc cgacggctac gaggcgttcc
     3481 gcggggtgga cgcatgagcg aggcgggcac gaccttggac gtcgcccgga tccgcaagga
     3541 cttcccgatc ctgtcccgcg acgtccgcgg cacccggctc gtctacctgg actcggccaa
     3601 cacctcccag aagccccagt cggtgctcga cgccatggac cactactacg agcggtccaa
     3661 cgccaacgtc caccgtggca cctacctcat cgcccaggag gccaccgacg cactcgaggg
     3721 cgcccgcggc atcgtgcgcc gcttcatcgg cgccccgtcg gaccgcaccg tcgtcttcac
     3781 caagaacgcc accgaggcgc tgaacctggt ggtgcgcgcg tggggcacca agcacctgcg
     3841 ggccggcgac gccgtcgtgc tcaccgagat ggagcaccac gccaacatcg tgccgtggca
     3901 ccagctggcc gaggccatcg gcatcgagct gcgctggatc ccggtgaccg acgagggcca
     3961 cctcgacgtc agcgacctcg atcgcctcct cgacggggcc aaggtcctgt ccttcaccgc
     4021 catgtccaat gtgctcggca cggtgacgcc ggtggccgag ctggtcgcca aggcccacga
     4081 cgccggtgcc atcgccatca tcgacgcgag ccagcacgtg cctcacatca ccaccgacgt
     4141 gcaggcgtgg ggcgcggact tcgtggcctt caccggtcac aagctgctcg gccccaccgg
     4201 catcggcgtg ctctacggca ccgaggagat cctcgaggcc accccgccct tcctcggcgg
     4261 cggcgagatg atcctcaacg tcaccaagga cgggttcacc cacaccgagc tgccctggaa
     4321 gttcgaggcc ggcacgccca tgatcgccga ggccgtcggc ctcggcgccg cgtgcgagta
     4381 cctcaccggc ctcggcatgg acgcggtgcg ccagcacgag gtcgagctca ccgcctacac
     4441 cctgcgcacc ctcaccgagc gcttcggcga cgacgtcacc atccacggcc cgagcgagcc
     4501 cgcggcccgc ggcggggtgt tctccttcgc ctacaaggac atccaccccc acgacctgac
     4561 ccaggtgttc gacgagcacg gcgtgtgcgt gcgggccggc caccactgcg ccaagcccct
     4621 catgcgcgcc ctcggcgtga acgccaccgc acgggcgtcg ctgtacgtgt acaacgacac
     4681 cgacgacatc gacgccctcg ccgacgccat ggacgcggcc ggccagttct tcagcttcta
     4741 aggatcaccg atgccaggac tcgaagacct ctaccgcgag atcatcctcg accactaccg
     4801 gtcgccccgg aacaagggcg agctggaggc cccgccggcc acccgcgtgg aggggttcaa
     4861 ccccctgtgc ggtgacgaga tcgtggtcta cgtcgacgtc gaggacgaca cggtcaagga
     4921 catccgcatc ggcggccagg gctgctcgat cagccagtcg tccgcgtcga tgatgtcggc
     4981 tgcggtcaag ggcaagacgc tcgaggaggt caagggcctc accaaggcgt tcaaggcgat
     5041 gatgtccatc cacgagcaga gcctcgacgg tgagggcgaa ggcctggagg agtccggcga
     5101 gtccggcatc gacccgaacg agatcgccga cgtgcccgag gaggtcaagc tcggcgacct
     5161 cgaggcactc cgcggcgtgg tgaagttccc ggtgcgcatc aagtgcgcca cgctgtcttg
     5221 gaacaccctg gcccagggca tcgacgaggc gacggcgggc tgaaccaccc tcagtcggtg
     5281 gggcgctccg tcgggggtgc cacccgcacg gtgccgcgct cgctgcgctt cacctcgtag
     5341 agcatcttgt cggcgcggtc gatgaactcg gccggcgtct cgccctgttc gggggtggcg
     5401 atgccgaccg acaggccggc gccgaagtcc gccatcaccg acgcagccgc ctcgcacatg
     5461 cgccggccga tgtcggccgc gccgtcggcg tcgacgtcct gcaggagggc ggcgaactcg
     5521 tcaccgccga tgcggaacat gcggtcctcg ctgcggagca cgtcgtgcag gacggcggcc
     5581 aggctgcgga gggcgaagtc gccggtgagg tggccgtggg tgtcgttgat gagcttgagg
     5641 ccgtcgacgt cggccatggc cagcgcccac ccgccacgcc ggcgggcgcc catgttcgac
     5701 agggcctcct ggaacgcgga caggttgccg aggccggtga gggtgtcgac ggtggcgcgg
     5761 gtgcggaggt cgtcgacgag cacgaccatg tcgagggctc gacccagctc gctggcgagc
     5821 agctcgagcg cctcgcggca gtcgagcccg agcgccatcg gggcgtggtc ggcgaggagc
     5881 agcaagccga cgccacgctc acccgagcgc accgggaggg ccaccaccga ggaggcacct
     5941 gcctcgcaca gcttcaccag ctcgggtcgc atcccgctcc ggccggccgc cacgaagcag
     6001 ctcgaggccg agccgacgtg gtcgagcagg ctgcgcagcg tctcggggct gagctggcgc
     6061 agctcgggag cgagtgggcc ctgcagcgac ttgatgtcga ggccggtggt gccgggcagg
     6121 gcgaacagcg tcgacgacat cccgccgacc accccggcga ggcgggcggc ggcgatctcc
     6181 acctggtgga cgtcggtgag cagggccagc tcgttggtgg cccgggccag caggtgcatg
     6241 gcggtctcgg ctcggggcga tccgacctcg ccgatccggg cgccggcgat gccgaccagc
     6301 tcgatcaggt gggactcctg ctcggtggtg aagcccgact cgccgaccgc ggtgacgacg
     6361 ccgacggcgc ggtcggcgac gtggatcggg tgggcgagac ggacgccctc gccgtgctcg
     6421 tcatcgcccc gcaggaggcc gcgcgcccgg aatgccgagc cgaggagccc cgagcgcacc
     6481 gggatgccgt cgagcacctc ggcgtgaccg tgctcggcga cgcagcggag gaggccgtcg
     6541 cgctcgaagt acaccgccga gcggtcgccg atgcgctcga gctcggcgac cagctgctcg
     6601 gccacggcgt tggcgtcgcc ggcggccccg atggactgct tggcctggcg caccgtgtcg
     6661 aggaccaggg gcggccggcg ccggcggggg ctgggcgggg cccagtcgcc ggtgggctgg
     6721 ccgaagagcg ggccgcgggc cagcaccacg ccgagggccc gcagctcgtc gagctggtcg
     6781 tgtcgttcga ccccttcggc gatgacgtgg atgccctcgt gctcggcgaa ggccacgtag
     6841 gcccgcacca ccgagcggtt ggccggatcg tggacgatgt cgcgcacgat cggcgggtcg
     6901 agcttcacga aggtggggcg gacgcgggcg atcagcgcca ggctcacgcc cgaggtgttc
     6961 tcgatcgaga ggtggatgcc ggccgtcgcc agcgcgtcga ggtcggagga cagttcgacg
     7021 aggcgatcga cctcggcgcc gcgcacgtcg accacgaccc gcgagcccag ccgctcgcac
     7081 tcctcgtaca gccgcgggtc gccgagcagg tcggcgctga cgtgcacgaa cacgagcccg
     7141 tcgtcgggcg gtgacccggc ggcctcgagc gacgcgagca ccgcgagacc gccgtcgggc
     7201 gcgtcgtcga cccaccggtg cggccgggcc tcccagccga tggggcgtcc ggtgcggagg
     7261 tcgacgatgg gctggaagcg ggcgcgacgg gccggggccc cgacagcgtc gtcgggccca
     7321 gcctgcgctg aggcctctcg cccgcttgtc acgccgtcct ccacgtcgat ccgcacgccg
     7381 gccgggcgga ccccacggtc tgccggtaca actggcgctc tcccggaccg gactgtacgc
     7441 gatccggatc ccttgatttc ggaccctgaa cgcattggtt cgaacgccct acgcgttcat
     7501 tgggcgcaaa gcggcaggaa tcggaccctc cgcacgctcc ggagtggtca gccctccgcc
     7561 ccgtagccgg atgcttccag ctcctcggcg atctcggggc cgccgctgcg ctcgatccgg
     7621 ccggcgacga gcacgtgcac cacgtcgggg cgcagctcgg tgaagaggcg ccggtagtgc
     7681 gtgatggcga gcaccccgag gccctcctcg gtgagctgct cgatgcggcg gctcacgtcg
     7741 cgcagggcgt cgatgtcgag accggagtcg atctcgtcga ggatcgcgat gcggggccgg
     7801 accgtggcca gctgcaccgt ctcgttgcgc ttcttctcgc caccggagag gtcgacgttg
     7861 agcggccggt ccaggaaccg ggcttcgaag ccgatgttgg ccgcctcggc ggcgatggtg
     7921 tcccgcaccg atccggggtc gcgcccggcg gccacggcgg actcgacgag catctcctcc
     7981 aggcggacac cagggacctc gaccgggtac tgcatggcga ggaacaggcc ggccttggcc
     8041 cgctcccagg cgggcatggc gagcaggtcg acgccgtcga gggtggcggt gccaccggtg
     8101 acggtgaagc tcgggtggcc ggcgagcacg ccggacaggg tcgacttgcc ggcgccgttg
     8161 ggacccatca cggcgtgccc ctcgcccgag gagacggtga ggtcgacgcc cttcaggatc
     8221 tccttgcccg ccacgtcggc ggtgaggccc ttgatctcga gggtgctcat gcgtccagct
     8281 ccacgtacac gtcgccgtcc ttcacgatca cttcgtaggt cggggtcggt ttggtggccg
     8341 ggagcacggt gggcacgccg tcgtcgaggc ggaacgtgct gccgtgcttc cagcactcga
     8401 tggtgccgtc ctcgcagtcg acctcgccct ccgacaggga gatgtcggcg tgcgtgcacc
     8461 ggtcgccgat gacgtagacc tcgtcgccgc accggacgat ggcgaggcgg tggccgtcga
     8521 tgtcgacgcg gatcgcgctg tcgggtccga cgtcgtcgag ggagcagatg cgggtgctca
     8581 tggcatggtc ctctcgagct tggcctcgag cgagtcgcgg accacgtcct cgaggctgcc
     8641 gagggcgagg ccgtcgagga cctcgtcgaa gaacccccgc acgatcaggc gctcggcccg
     8701 gccggtgggc acgccgcggc tctcgaggta gaagcgctgc tcggcgtcga cggggccgac
     8761 ggtcgaggcg tggctgcact gcacctcgtt gttctcgatc tcgaggttcg gcaccgactc
     8821 ggcccaggcg tcgtcggaca gcttgatgtt gcggttggtc tggaacgcct gggtgccgcg
     8881 ggccgtgggg cggatcttga tgagaccggt gtagaccgat gcgcccttct cccccagcgc
     8941 ccccttgaac agcaggttcg aggtggtgtc cggggcgacg tggtcctgga aggtgcggaa
     9001 gtccagcagc tgggagccgt cgccgaagta ggcagcggtg atctcgccgt gggcgccgcg
     9061 accggcgagc gtggtgtcga tgcgggtgcg ggcgtagtcg ccgccgaagg ccgcggtgcc
     9121 ggcgtgcagg gtgccgccct ggtcgacggt ggaggccagc cggccgatct gccaggcgcc
     9181 gtcgccgagg agctgcaggg tgcggtggcg cagcgtggcg tcccgtccga ccgcgagctc
     9241 ggtgacgggg agggcgaggg actgcacgcc atcggggccg tggtggacct cgacgaccga
     9301 ggcgctggac gcctggccga gctcgaccac gagccggggg aaggaggcga cgccctcggg
     9361 cccgctggtg tgcacgacca cgacctgacc ggcgtcggcg ccagccggca cctggacgac
     9421 gatcggggcg gcgaaggcgt cgtgcagcgc gctcagcgag tcggtggtgt cgccgagcac
     9481 ggagccgagc ggaccgtcgg cggccaggtc gtcgcccgtg gtgacctgca ccttggcccc
     9541 gtcggcgagg tcgacgcgcg tgacctgtcc ggccacggtc cacaccacgg cggaggcgcc
     9601 ggcggtgagc tcgagcagca tgtcggcccg gccgtggtcg gctcccgtgg cgtcgtcggc
     9661 cgtgctgggt cggtagcggt cgagggccag atcaccgatg cggctgtagc gccacacctc
     9721 ctcctcggcg gacgggagct cggcgccggc gaagcgctcg gccgctgcac ggcggcggtc
     9781 gaccagccac tgcggcccgg ggagggcagc ggcggcgtcg ggcgagaacg tggaggtcat
     9841 cgtggtcagg gcgtccctct ggggagaacg ggggcgggcg cgaagcggag gccgagacga
     9901 cctccgtggg ggcgagccta ccgaggggac cgcccggttc cccagcgccg gacaggcggt
     9961 ccggccctag gaatccttct tgcgcgagcg cttgaacaac cgcttccagc gcgggctctc
    10021 cgctgcccgg cgctcccgct cgacgtcggc caccatctcg gcgccgaccg cgttgacgat
    10081 gtcctcggcg gcgtcgagca gggcagcgat ggagccgtcg tcctcgtcga gcccgggggg
    10141 ttccaccggg gtctcgggcg tcaccagcgt gccgtgctgc cagttcacgt cgaccaggcg
    10201 cggcgcgtac ttcgggcagt cctcggggca ccgccacggg gcgtggggcg cgaggtcgag
    10261 gtcgcacttc cgcacggtgt cgccgttcgg gtacgtgcgg gattcgaagt acttgcagtc
    10321 ctgccgcatc ggcatggcgt cagtgtggca gcgggtgatc tcccctccac agcagggccg
    10381 gcaggaggtg gggggaccgg cgccgaatgc ggtcgggtgg acccaggcca ggacgcggac
    10441 agcgacgtcg cgccggaagc gaccggctgg ggcgccctcg gacgcaacca caagctgctg
    10501 ctcgccctgc tcggcgcgac cagcttcttc gacggcttcg accggggcat cctcaccgtg
    10561 gcgctccccc agatccgcga cacctacgac ctggaccagg gccagatgtc gttgtggatc
    10621 tcgctcctct acgtcggcgc gctgccggcc ctgctcctga cccggcgggc ggacaaggtc
    10681 ggccgacgcc ggctcctgct catctcgatc gtcgggtaca cgatcgccac ggggctcacc
    10741 gcggtggcgc ccagcatcga gtggttcgtc gcgttccagt tcatcgcccg cctgttcctc
    10801 aacgccgagc acgccttggt cttcaccctc gccgccgagg agcttccggc gaaggcccgt
    10861 ggcttcggct tcggcttcct gtcgatgaac ctggcgctcg gggtcgggct gtcggcgatc
    10921 gtgttcggca cggtcttcga gccggcgggc atctcctggc gcgccatgta cctggtcggg
    10981 ttgcccccgc tcgtcctcat cggctggctg cggcgacgga tcccggagtc caggcgcttc
    11041 gaggccgccc gtgacggcgg cgagctcacc gggagctggc gggccatcct ggcgccggga
    11101 caccggcggt ggctgctgct cctgtgtgcc accgccttcc tcctcgagct ggggacccat
    11161 gcgtcggtct tcacgcagga cttcctgcag gaggacaagg ggctcagcgc cacgctggcc
    11221 agcctgatgc tcgtggccgc ggggttgccg gggatcccga tcatggtgtg ggccggcggc
    11281 ctgtcggacc ggctcggtcg gcgcctcgtc gggtgctccc tcgccgcggt cggtgtggtg
    11341 ggcgccctcg gcttcttctg gttgccgggt ggcgtgccgg tgctgctcgc gttcatggcg
    11401 ctcacgctcg tcggccagat gggtgcgggt ccggtgctcg gcacctacgc gaccgagctg
    11461 ttccccaccg cactgcgcgg tcaggccggg tcgtgggcca aggtcgccgc cgtcggaggg
    11521 caagccggct ccctcgcact cggcggtgtc ctgatctcgc tcaccggcgg cctgccgggg
    11581 gcggccacgg tgctcatgat cggaccgatc gtggccatcg ccgtgttcgc cgtgtccttc
    11641 cccgacaccc acggccgcga gctcgaggac accgccgggg gctaccccga cgtcgacggc
    11701 gtcgccgccg agctcgccac cccgatctga tcgccgcgtc gggcaggctc gttgcgtgac
    11761 cgaaccgaca cggatcatcc gcgacgtcga cggcatcgcc cacgtgcgcg ccgcctcggt
    11821 cctcgccgcc ttcgagggtc agggcttcgc cgcgtccgag gaccggatct ggcagatgga
    11881 gctggatcgc cgtcgggcgc tcggcaccct cgccgaggtg gtgggaccgt ccgccgtcgc
    11941 cgtcgacacc ttccaccggc gcatgggtct ggccacccac gcgcgggcga gcctcgacgc
    12001 gctcgacgac gagacccgcg ccgtgctcga tgcctacgcc gccggcgtga accgcgggtt
    12061 ggattcggac gccgacctgc caccggagct cgtcgccctc ggggtcgtgc tcgcgccctg
    12121 ggaggcgtgg cactcgatcg ccctctacga ggtccgccac ctggcgatgg gcacctacga
    12181 gaccaagctc tggcgcagcg gcctcgtcca ccgcctcggc gccgaggccg tggcccggct
    12241 gtggccgacc accgacgagg tgctcgtcga cccgaccgct cccgacgcac ccgtgcccat
    12301 cgacgtggcc gccgcgctgc cggccggcga gggcctgctg tcccgcttct ccctcgccga
    12361 cgtcggcggc accggtgagc acggctccaa caacctcgtc atcgggccgt cgctcactgc
    12421 gaccgggcgg ccgatcctgg ccggcgaccc gcaccgagcg atcgacctgc ccaacgtcta
    12481 ctggcagaac catctcacct gcaccgaccc cgacgacccg ctcgacgtca tcggcctctc
    12541 gttccccggc gtgcccggct tcccgcactt cggccacaac gcggacgtcg cctggtgcat
    12601 cacccacggc atggccgacg accaggacct gtcggtcgtc gacctgcgcc gcaccggcga
    12661 tggggtcgag caccaccgcg ccgacggctg gcacgccgcc gatgtccgca ccgaggaggt
    12721 cgccgtcgcc ggcggggacg gcatcgaggt cgagtgcgtc accacacccg ccggcccggt
    12781 ggtggcatct ctcgagcggg acggcacctg gaccggcatc gccctgcgct ggacggcgac
    12841 cgcggcggtg gacaccaccg ccgccgcgct cctccccatg gtccgggcct gctcggtcga
    12901 cgacctcgac cgcgccttcg agccctgggt ggtcccggtc aacaacgtgc tgatggccga
    12961 caccgccggc gccatcgcct accggatgcg gggtcgcgtc gcggtgcggg ccgcatcgtc
    13021 cggctggacg gtggtggacg gcgccgatcc agccacggcg tgggagggct tcgtgcccga
    13081 cgccgacctg ccccggtggc gggaccccga ccggggcttc ctggtcacgt cgaacaaccg
    13141 catcgccgcc gacggcccct acgtctccca cgactgggcc cacccgaccc gggcacgccg
    13201 catcaccgag ctgctcgctg gtcgtgacgg gtggagcgtg gacgacgtcg gtgccctgct
    13261 cggtgacacc cactcgggcg tcgccgccgt gctggcccgc cgcctgggcg cgctgtcgcc
    13321 ctcgcacccg atggaggtgc gtgccctcgc cctcctcgac ggctgggacc accacatgga
    13381 cgtcgcgtcg tcggccgccg cggtcgtcgg gacggtgcgc ggcgagctgc tgggcctgct
    13441 cgtccacgag ctcggtctcg ccgacgaccg cctccccggt gtttccggcc cgtcgctgca
    13501 ccagaccatg cgcttcgcca acgcccggct ggcctggtgg atcgacgacc ccggcctggt
    13561 gcgcgaccgc accctctcgt ccgccctgcg cctggcggtc cggcagctcg agctgaccca
    13621 ggggcccgac ccgtcccgct ggcgctgggg cgcggcccac ctggcccgct gggcccaccc
    13681 gctgctcgcg ctgcgaccgg acctggccga ccaggtcgtc gtaccaccga cggtcgagct
    13741 cggtggcgac aacgagtgcg tgtgggccac ctccaccgcc ccgccgtcca ccgaggcatc
    13801 gaacggccag gtcgcccgct acgtgttcga cgtcggcgac tgggaccggt cgcgctggat
    13861 cgtgccccac ggcgtctccg gcgacagccg ctccgcacac cacctcgacc agctcgagga
    13921 ctgggcgggc atgcgcctcc gaccgatgcg ctggtccgcc gacgtcgtcg acgccgccac
    13981 cgagtccgtc accgccctcc cctgaggacc tctcccgaac tggcagtgct ggtgtcacgc
    14041 gcggtgcccg cggcgcccac caccgctgac ggtcaaccgg ggatcaagcc gtggttcgag
    14101 ttctcgatgg tgatgtcccc gatgacgacc cgtgccccgt ccggtcgctc ggcgacgagg
    14161 ccggtggcga ccagcgtcgc cggaccccac gcgccgtcgg gcggcaggac gacctcgatc
    14221 gaaccctcca ccaccggcca ctcctcgagc acctccgggg tcggctcccc cggctcgagc
    14281 acgtcgtcgc accagttgcc gaacaggtcc tgcccgaggc gcacggtgcc ggtccagctg
    14341 ccaccgggca gcacgt
//
LOCUS       DATISU010000141         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_146539, whole genome shotgun sequence.
ACCESSION   DATISU010000141 DATISU010000000
VERSION     DATISU010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146539"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VLR27_05905"
     CDS             <1..1086
                     /locus_tag="VLR27_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSP03015.1"
                     /translation="LAAGDLRDAALLLHVHGPRLLRAGQVETLGRLLSRLGEVARADV
                     GCAVLAGWCAFFAGRYAEAEQWLGVVLELEPAGLPGATSLRINLSLATGDVTGALDVA
                     RDELAACLRDGGPPELATAVGAAHGWAGVVDEARAALEVAAAGADATHNWSAQTVSLV
                     HRCVAELEHSAPAVAHRAAEAAIETAERFGLAGYHGVAPAFAVRARTGDDPGRARDDV
                     AYALASARRSSTPLALGFVLALCGDTLLDLGDDAGSALLEEARSMLDRCPDPGLAGRH
                     LARAEARHGTPTPRPAPAADLVEQLTDRELAVLRYLPSGLSQRDIALELYVSINTVRT
                     HCRAIYRKLGVGDRHAAVQAARDHRLL"
     gene            1115..>2364
                     /locus_tag="VLR27_05910"
     CDS             1115..>2364
                     /locus_tag="VLR27_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSP03016.1"
                     /translation="MAEPGTVDVVEVLRGVLPDGLVRSGTDAGDDFHHDEALGIEPVI
                     PAAVVTPETEDHVAAVLAACDASGIPVTARGSGTGLSGAAIPHPDGTVIAFDRMAEII
                     EIDEDNLVAVVQPGVTLDQLDQALAPRGLVYPVFPGEYSASLGGNVATNAGGMRAVKY
                     GVTRHQVLGLRAVLPSGEVITTGGKFVKGSTGYDLTQLIIGSEGTLAVVTEAILKLYP
                     RPEHRATVLAPFTTLDQVTRAVPAIVKSGIGPMILEYIDMLTMSGIENTLGVEVGVPE
                     DIKATALAYLVVQLEDRHEDRLDADTQALAELVADLGAIDVYVLPSNAATQLIEAREK
                     AFWLAKGAGAHDIVDVVVPRASIDPFLTAVSQLAQDKQTFIAGCGHAGDGNVHLSVFQ
                     PDPDVRYEMMLELFRLGMDHGGAIS"
BASE COUNT          307 a          936 c          815 g          306 t
ORIGIN      
        1 ctggccgccg gcgacctccg agacgccgcg ctgctgctgc acgtccacgg tccccggttg
       61 ctgcgggccg gtcaggtcga gacgctcggc cggttgctgt cccgtctggg cgaggtcgcc
      121 cgcgccgacg tgggctgcgc ggtcctggcc ggctggtgcg cgttcttcgc cggtcgctac
      181 gccgaggccg agcagtggct cggcgtcgtg ctggagctgg agcccgccgg gctcccgggg
      241 gccacgtcgc tgcgcatcaa cctctcgctg gcgaccggtg acgtcactgg cgcgctcgac
      301 gtggcgaggg acgagctggc tgcgtgcctg cgtgacggcg gcccgccgga gctggccacc
      361 gccgtcggcg ccgcccacgg ctgggccggc gtggtcgacg aggcccgcgc ggccctcgag
      421 gtcgcggcgg ccggtgccga cgccacgcac aactggtcgg cgcagaccgt ctccctcgtc
      481 caccgctgcg tggccgagct cgagcacagc gcgcccgccg tcgcccaccg ggcggccgag
      541 gcggcgatcg agaccgccga gcggttcggg ctggcgggct accacggcgt ggcgccggcg
      601 ttcgccgtcc gggcccgcac gggtgacgac ccgggccggg cccgcgacga cgtcgcctac
      661 gccctggcca gcgcccgtcg ctcctccacc ccactcgccc tcggcttcgt cctcgccctg
      721 tgcggcgaca cgctcctcga cctcggcgac gacgccggat cggcgttgct cgaggaggcc
      781 cgctcgatgc tcgaccgctg cccggacccc ggcctcgccg gccgccacct cgcccgggcc
      841 gaggcccgcc acggcacccc gaccccccga cctgcgccgg ccgccgacct ggtcgagcag
      901 ctcaccgacc gggagctggc cgtgctccgc tacctgccga gcgggctgag ccagcgcgac
      961 atcgccctgg agctgtacgt gtcgatcaac accgtgcgca cccactgccg ggccatctac
     1021 cgcaagctcg gggtcggcga ccgccacgcc gccgtgcaag cggcgcggga ccaccggctg
     1081 ctctgaccac cgccagcccg taccatccgc ggacatggcc gagccgggga ccgtggacgt
     1141 cgtcgaggtg ctgcggggcg tcctgcccga cggcctggtc cgcagcggca cggacgccgg
     1201 cgacgacttc caccacgacg aggcgctcgg catcgagccc gtgatcccgg ccgcggtcgt
     1261 cacccccgag accgaggatc acgtggcagc cgtcctcgcc gcctgcgacg cgagcggcat
     1321 ccccgtcacc gcccggggca gcggcaccgg gctgtccggc gcagccatcc cgcaccccga
     1381 cgggaccgtg atcgccttcg accggatggc cgagatcatc gagatcgacg aggacaacct
     1441 cgtggcggtc gtgcaaccgg gcgtcaccct cgaccagctc gaccaggcgc tcgcgccccg
     1501 ggggctcgtc tacccggtgt tccccgggga gtacagcgcc agcctcggcg gcaacgtggc
     1561 caccaacgcc ggcggcatgc gggccgtgaa gtacggggtc acccgacacc aggtgctcgg
     1621 cctgcgcgcc gtgctgccgt ccggcgaggt catcaccacc ggcggcaagt tcgtcaaggg
     1681 ctccaccggc tacgacctca cccagctcat catcggctcg gaggggaccc tggccgtggt
     1741 caccgaggcg atcctcaagc tctacccgcg ccccgagcac cgggccacgg tgctggcacc
     1801 gttcaccacc ctcgaccagg tcacccgggc cgtgcccgcc atcgtcaaga gcggcatcgg
     1861 cccgatgatc ctcgagtaca tcgacatgct caccatgtcc ggcatcgaga acaccctcgg
     1921 cgtggaggtc ggtgtccccg aggacatcaa ggccaccgcc ctcgcctacc tggtggtcca
     1981 gctcgaggac cgccacgagg accgcctcga cgccgacacc caggcgctgg ccgagctggt
     2041 cgccgatctc ggcgcgatcg acgtgtacgt gctgccgtcc aacgccgcca cccagctgat
     2101 cgaggcccgg gagaaggcgt tctggctggc caagggcgcg ggcgcccacg acatcgtcga
     2161 cgtggtggtg ccgcgggcgt cgatcgaccc gttcctcacc gcggtgtcgc agctggcgca
     2221 ggacaagcag accttcatcg ccggctgcgg ccacgccggc gacggcaacg tgcacctgtc
     2281 ggtgttccag cccgaccccg acgtgcgcta cgagatgatg ctcgagctgt tccgcctcgg
     2341 catggaccac ggcggggcga tctc
//
LOCUS       DATISU010000142         4847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_332535, whole genome shotgun sequence.
ACCESSION   DATISU010000142 DATISU010000000
VERSION     DATISU010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332535"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VLR27_05915"
     CDS             complement(<1..402)
                     /locus_tag="VLR27_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03017.1"
                     /translation="MSEVPVTRAQIDELLDAAGAVRNRDLLADIFRTAVGLAGDDITR
                     LDLKIASASLHEMRLAFNTFRPYADRSKVTMFGSARTQPDDAEYHQTRRLAADFAERG
                     WMVVTGAGPGIMAAGMEGAGRENSIGVTIRLP"
     gene            508..888
                     /locus_tag="VLR27_05920"
     CDS             508..888
                     /locus_tag="VLR27_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSP03018.1"
                     /translation="MSSVLVVDDEPDVRLVTRVILEASGYDVWEAASGEDALDVLGGG
                     DLPDVLLLDVRMPGIDGWEVLRRVRGAPGLLCDLPVVIFTADIASAEQAPVALDDREF
                     FLSKPFDPDDLIDMVGQAAKTASV"
     gene            complement(896..3157)
                     /gene="cofH"
                     /locus_tag="VLR27_05925"
     CDS             complement(896..3157)
                     /gene="cofH"
                     /locus_tag="VLR27_05925"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HSP03019.1"
                     /translation="MVDQTHPLLALPLDELQARARAVRDGRTGTRITYSPKVFIPLTM
                     LCRDKCGYCTVAQPPARLESPYLTPDQVLAIARAGAEAGCHEALFTLGERPEDRYPAA
                     ADWLAANGYDSTVDYLAAMCRLVLEETGLLPHANAGALYADELAKLRPVAPSQGMMLE
                     TLRDDLECHRGSPDKVPARRLATLEAAGELGIAFTTGILVGIGEDPIDRVRALEAIAE
                     SHARHGHVQEVIVQNFLPKPGTGMHKAAPCPPEDFLQAIALARLILPPEIHLQAPPNL
                     SDDFGELLDAGIDDWGGVSPVTADHVNPERPWPALERLREVTEARGFTLAPRLTIYPE
                     HALAPERFLDEGLRFPVLDRSDAEAMGRDDRWYSGHEDAPPVLVPGHAAAGGAVGEVL
                     AGALLGQELGVEEITTLFGARGPEVAAVAQVADEMRREAVGDVVTWVQNRNINYTNVC
                     TFKCRFCGFSKGPLSLNLRGNPYLLTLDEIAGRVKEAWDMGATEVCLQGGIHPDFDGD
                     YYIDVTKAVKEAAPGIHVHGFTALEVTEGAKRLGEPLADYLRRLMDVGLATLPGTAAE
                     ILDDDMRALLCPDKIDTEEWLDAHRTAHQVGLRSNVTIMYGAIENPVHWARHIVRTRD
                     LQRETGGFTEFVPLPFVHMAAPMYIQRKARRGPTFREALLMHAVGRIAYRGAIDNIQV
                     SWVKMGRSGVTQCLQAGVNDLGGTLMDENISRAAGANHGQMMEGDDFRSIVEPLGRRL
                     EQRTTLYGRTAPA"
     gene            3196..4071
                     /locus_tag="VLR27_05930"
     CDS             3196..4071
                     /locus_tag="VLR27_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03020.1"
                     /translation="MPPAGAVTVEPVESKSQLRRFAEVPFVLLGTDDRWSAGVRAHQQ
                     WRHDARRHPYFDRGDAAFFLARRGGQPAGRIAAHVDGTRSEQGWFGMFDVADDDAVTA
                     ALVDAATGWLREQAVTSMTGPVTWTPDEDFGVLVEGYEHPATTGRAWHPPWYAEQLRS
                     AGASPGERRATFHLSTEDWAGEVPTPSGEDPPPQAGGYADPALVLDGIAAVPDVTRLL
                     AGASVRSAWRLARRARERASDIAVCVRCDGDPSVLVPRLLTACRARGYSTLLSPWTPD
                     GGTPDRVHQVFRFTV"
     gene            4135..4374
                     /locus_tag="VLR27_05935"
     CDS             4135..4374
                     /locus_tag="VLR27_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03021.1"
                     /translation="MEINDSAHRHDVSEEDMLHAVEHSLVVDDLGEDPDRWLVLGPDT
                     AGNLLELVVLVSTEGDEIIIHAMPMRPKYRRLLER"
     gene            4371..4658
                     /locus_tag="VLR27_05940"
     CDS             4371..4658
                     /locus_tag="VLR27_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HSP03022.1"
                     /translation="MTAEHHGHTKSGTPLTDELIEKLADEAEAGYDVDEILGRRSKRG
                     RPSLGAGPSTVESVRLDPELKERLAQRAEKEGVPVSEVIREALRHHLDAAS"
BASE COUNT          653 a         1616 c         1858 g          720 t
ORIGIN      
        1 gggcagccgg atggtgacgc cgatggagtt ctcccggccg gcgccctcca tgccggcggc
       61 catgatcccg gggccggcgc cggtgaccac catccagccc cgctcggcga agtcggcggc
      121 caggcggcgg gtctggtggt actcggcgtc gtcgggctgg gtgcgggccg agccgaacat
      181 ggtgaccttg gaccggtcgg cgtagggccg gaacgtgttg aacgccaggc gcatctcgtg
      241 cagggaggcg gacgcgatct tgaggtcgag ccgggtgatg tcgtcgccgg cgaggccgac
      301 ggcggtgcgg aagatgtccg cgagcaggtc gcggttgcgt acggcgccgg cggcatcgag
      361 gagctcgtcg atctgggctc gggtgacggg gacctccgac atcgcccgag catggcacag
      421 ggggcctccg ccgacgtccg gctccgtgcg cggtcgggtg accaccgtgc cgtagcctcc
      481 gagccggtcg gagggaatgg gggcctcgtg agctcggtgc tggtggtcga cgatgaaccg
      541 gatgtgcgcc tcgtgacgcg ggtcatcctc gaggcgtccg gctacgacgt gtgggaagcc
      601 gcgtcggggg aggacgccct cgacgtgctc ggcggaggcg acctgccgga cgtgctcctc
      661 ctggacgtgc gcatgcccgg catcgacggc tgggaggtgc tccgtcgggt ccggggcgcg
      721 cccggcctgc tctgcgacct gccggtggtg atcttcaccg ccgacatcgc ctcggccgag
      781 caggccccgg tggcactgga cgaccgcgag ttcttcctga gcaagccctt cgaccccgac
      841 gacctgatcg acatggtcgg ccaggccgcg aagacggctt cggtctgaga cgcgcctacg
      901 cgggtgcggt gcgcccgtag agcgtggtcc gctgctcgag gcggcgaccc aggggctcga
      961 cgatggagcg gaagtcgtcg ccttccatca tctgtccgtg gttggcgccg gcggcccgcg
     1021 agatgttctc gtccatgagc gtgccgccga ggtcgttcac cccggcctgc aggcactggg
     1081 tgaccccgga ccggcccatc ttcacccagg acacctggat gttgtcgatg gcgccccggt
     1141 aggcgatgcg cccgacggcg tgcatgagca gcgcttcgcg gaaggtgggg ccgcgacggg
     1201 ccttgcgctg gatgtacatg ggggcggcca tgtgcacgaa ggggaggggc acgaactcgg
     1261 tgaagccacc ggtctcgcgc tgcaggtcgc gggtgcgcac gatgtggcgc gcccagtgca
     1321 ccgggttctc gatggcgccg tacatgatcg tcacgttgga gcgcaggccg acctggtggg
     1381 ccgtgcggtg ggcgtcgagc cactcctcgg tgtcgatctt gtccgggcac agcagcgcac
     1441 gcatgtcgtc gtcgaggatc tcggcggccg tgccgggcag ggtggcgagg cccacgtcca
     1501 tgagccggcg caggtagtcg gccaggggct cgccgaggcg cttggcgccc tcggtgacct
     1561 cgagcgcggt gaagccgtgc acgtggatgc cgggtgcggc ctccttcacg gccttggtga
     1621 cgtcgatgta gtagtcgccg tcgaagtcgg ggtggatgcc gccctgcagg caaacctccg
     1681 tagcccccat gtcccaggcc tccttcaccc ggccggcgat ctcgtccagg gtgagcagat
     1741 acgggttgcc gcgcaggttg agcgacagcg gccccttgga gaacccgcag aaccggcact
     1801 tgaaggtgca cacgttcgtg tagttgatgt tgcggttctg gacccacgtg acgacgtcgc
     1861 cgacggcctc ccgtcgcatc tcgtcggcga cctgggcgac ggcggcgacc tcggggccgc
     1921 gggcgccgaa cagggtcgtg atctcctcga caccgagctc ctggccgagg agggccccgg
     1981 cgaggacctc gccgaccgca ccgcccgccg ccgcgtggcc gggcaccagc accggcgggg
     2041 cgtcctcatg gcccgagtac cagcggtcgt cgcggcccat ggcctcggcg tcgctgcggt
     2101 cgaggacggg gaagcggagg ccctcgtcga ggaagcgctc cggcgccagc gcgtgctcgg
     2161 ggtagatggt gaggcggggg gcgagggtga agccgcgcgc ctcggtgacc tcccggaggc
     2221 gctcgagcgc cggccagggg cgctcggggt tcacgtggtc agcggtgacg ggcgagacgc
     2281 cgccccagtc gtcgatgccg gcgtcgagca gctcgccgaa gtcgtcggag aggttcggcg
     2341 gggcctggag gtggatctcc ggcgggagga tgagccgggc gagggcgatg gcctggagga
     2401 agtcctcggg cgggcacggt gccgccttgt gcatcccggt gccgggcttg ggcaggaagt
     2461 tctggacgat cacctcctgc acgtggccgt ggcgggcgtg gctctcggcg atggcctcga
     2521 gggcccggac ccggtcgatg gggtcctcgc cgatgccgac gaggatgccc gtggtgaagg
     2581 cgatgccgag ctcgccggcc gcctcgagcg tggccaggcg gcgggcgggc accttgtcgg
     2641 gtgagccgcg gtggcactcg aggtcgtcgc ggagggtctc gagcatcatc ccctgggacg
     2701 gggcgaccgg ccggagcttc gccagctcgt cggcgtacag ggcgccggcg ttggcgtggg
     2761 ggagcaggcc ggtctcctcg agcacgagac ggcacatggc ggcgaggtag tcgacggtcg
     2821 agtcgtaccc gttggcggcc agccaatcgg ccgcggcggg gtagcgatcc tcggggcgct
     2881 cgccgagggt gaacagcgcc tcgtggcagc cggcctcggc cccggcccgg gcgatggcca
     2941 gcacctggtc gggcgtgagg tacggggact ccagccgggc gggcggctgg gcgaccgtgc
     3001 agtagccgca cttgtcccgg cacagcatgg tcagcgggat gaagaccttg ggggagtagg
     3061 tgatgcgggt gcccgtccgc ccgtcgcgca cggcccgggc tcgggcctgc agctcgtcga
     3121 gggggagcgc cagcagcggg tgcgtctggt ccaccatggg cagaccctat cggtgggtgg
     3181 gcctaccctc gccgtgtgcc ccccgcaggt gccgtcaccg tcgagccggt cgagtccaag
     3241 tcgcagctgc ggcgattcgc ggaggtcccg ttcgtgctgc tcgggaccga cgaccggtgg
     3301 tcggcgggcg ttcgcgccca ccagcagtgg cgccacgacg cccggcgcca cccttacttc
     3361 gaccgtggcg acgccgcctt cttcctggcg cgccggggcg gccagcccgc cggacggatc
     3421 gccgcccacg tggacgggac cagatcggag cagggctggt tcgggatgtt cgacgtcgcc
     3481 gacgacgacg cggtgaccgc cgcgctcgtg gacgccgcga cggggtggct gcgcgaacag
     3541 gcggtgacgt cgatgaccgg accggtgacc tggacccccg acgaggactt cggtgtgctg
     3601 gtcgaggggt acgagcaccc ggccaccacc ggccgggcgt ggcacccgcc gtggtacgcc
     3661 gaacagctgc gctccgccgg cgcttcgcct ggggagcgtc gggcgacgtt ccacctctcg
     3721 accgaggact gggcgggcga ggttccgacc ccgagcgggg aggacccccc accgcaggcc
     3781 ggtggctacg ccgacccggc gttggtgctc gacgggatcg ctgccgtccc cgatgtcacc
     3841 cggctgctgg cgggggcctc cgtgcggtcg gcgtggcggc tggcccgccg ggcgcgggag
     3901 cgggcctcgg acatcgcggt ctgcgtgcgc tgcgacggcg atccgtcggt gctcgtgccc
     3961 cggctgctga cggcgtgccg ggcccgtggg tactcgacgc tgctgtcgcc gtggacgccc
     4021 gatggtggga cgccggaccg ggtgcaccag gtcttccggt tcacggtctg agcgaacacg
     4081 tcacgctgcg gcgcccgagc cggcactcgg tatttcgtag tacgctaatc gggtgtggag
     4141 atcaacgact cggcgcatcg ccacgatgtc tccgaggaag acatgctcca cgccgtcgag
     4201 cactcgctgg tcgtggacga cctcggcgag gacccggacc ggtggctggt cctcggaccc
     4261 gacaccgccg gcaacctcct cgaactcgtc gtcctcgtca gcaccgaagg tgacgagatc
     4321 atcatccatg cgatgccgat gcgaccgaag taccggaggc tcctcgaacg atgaccgctg
     4381 aacatcacgg acacaccaag tcgggcacgc ccctgaccga cgagctcatc gagaagctcg
     4441 ccgacgaggc cgaggccggc tacgacgtcg acgagatcct cggccggcgc agcaagcgag
     4501 ggcgaccgtc gcttggcgca ggaccgtcga cagtcgaatc ggtccgcttg gatcccgagt
     4561 tgaaggaacg gctcgcccaa cgggctgaga aggaaggagt gccggtctcc gaggtaatcc
     4621 gcgaggcgct tcgtcaccat ctcgacgccg catcctgacg ctgggctcga cccgtcagcg
     4681 ttgcgtcgca acggcctcga ggttggcgag tgtcgtccga atgttctgct cgttgtgggc
     4741 gcgaaggtca ctgatgccca tcaccgcccc ggcgaaggcc gccagccacc tcggccgatg
     4801 atccgaccag ctctcggtca cctccgtccg atcgcccgcc ggtagaa
//
LOCUS       DATISU010000143         3410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_519277, whole genome shotgun sequence.
ACCESSION   DATISU010000143 DATISU010000000
VERSION     DATISU010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519277"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..2409)
                     /locus_tag="VLR27_05945"
     CDS             complement(70..2409)
                     /locus_tag="VLR27_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03023.1"
                     /translation="MKSTTRRALVGIAAVAMFGALTPLASSTEEGAPPTDQATLASLP
                     QVASGERPGPDVLYDPAPVAPQLENRHSAFVAPFDRVSGTDRYVDGEYLYTDHIYDDS
                     GDGLSYPTDRGRFADNAADLVELRISDRLDGATHYRFTLNTLLAEDTTHVAVAFDTDR
                     DAATGSADLPDEPGAPFPGTDAVLSAWGTGASWSAWDGAAWQRQDLTSTVDLEANQIT
                     VAVPDAVAQPTGTWRATLAVGLHDPDAGGFLAPQATATETDPGGGDGKAAIFNLGFRF
                     DEAILTSSAPPDEDQSAALAAKEPTRYAHDIDFDAIAARQSGTTVPEHGTMVRFFPSR
                     LQLGEGRDDSSFPATLGQLQAYSLYVPSGYQPGTPAPLTLDLHSLGERHWQYNGSTGI
                     QQLGEERGSLVATADARGEDGWYQHEAEYDVFEMWNDIARHHTLDPDSTAITGYSMGG
                     YATYRLGTLYPDLFGSAVTVVGPPGDGIWVGAGAPTGGAETLSNLWLENARNVPFMNI
                     VAAADELVPITGTTQQNVGPAEGGRTSFDALGYRYAFDVYPAAEHLTLAVLGYDLPQA
                     AEFLGNAAVDRDPFHVTFAYTPAADDPELGLVHNKAYWVSDVALADPAVGVAVSSLED
                     PIGKGVVDVESLGFGIDDPTPTRGNGAGTVPLPYVETNQTWGEAPARDAENALELRLT
                     NVGAVTLDLARARITSEEPIELRHDGDNDAVVTLVDADGTRVDVAVPAGSGVIVVEPS
                     AFGHDDGDPQEPGGGDRPGRGDERRCVPDADARPRGCKP"
     gene            2527..>3410
                     /locus_tag="VLR27_05950"
     CDS             2527..>3410
                     /locus_tag="VLR27_05950"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HSP03024.1"
                     /translation="MFARLPPLARLALVSLVLIALAWAIFGRSVEDEIAVVPDTTDTT
                     EPSVFEPLDTSDTGVVLVDDLVLPVTGGEDGAWTVLTPCANAATVDGELLTGAHVVID
                     PGHGGRETGAIGPSGVVEAEINLDVALKAKALLEAEGATVALTRNDDTRVTLETRAAI
                     ARALDPLLFISIHHNGGPTRTAERPGVQVYHQHQTPESARFAGVLFEQLQEAMAPFSE
                     TWSGGNATGVRARLGEDGDDFYGVLRASEGVPAVLAEALYMSDEPEATLLLRDDVRQA
                     EAQAITDAIVAWLDSPYAG"
BASE COUNT          444 a         1189 c         1269 g          508 t
ORIGIN      
        1 tgtgaaggcg aaggcggact tcgatcagca gacggtcatc gatcggacgc tggccgtgta
       61 cgcgagggac tagggcttgc agccgcgcgg tcgggcatcg gcgtccggca cgcagcggcg
      121 ctcgtcgccc cgaccaggac ggtcaccgcc accgggttcc tggggatcgc cgtcgtcgtg
      181 cccgaaggcc gacggctcga cgacgatcac gcccgatccg gccgggaccg cgacgtccac
      241 ccgggtgccg tcggcgtcga ccagcgtgac gacggcgtcg ttgtcgccgt cgtggcgcag
      301 ctcgatcggc tcctcgctgg tgatccgagc ccgggccagg tcgagcgtga ccgcgccgac
      361 gttggtcagc cgcagctcga gcgcgttctc ggcgtcccgg gccggcgcct cgccccacgt
      421 ctggttcgtc tcgacgtagg gcagcgggac ggtcccggcg ccgttgcccc gggtcggggt
      481 cgggtcgtcg atgccgaacc ccaggctctc gacgtcgacc acgcccttgc cgatcgggtc
      541 ctccagggac gagacggcga cgcccacggc cggatcggcg agcgcgacgt cgctgaccca
      601 gtacgccttg ttgtgcacga ggccgagctc ggggtcgtcg gcggctggcg tgtaggcgaa
      661 ggtgacgtgg aacggatccc ggtcgacggc agcgttgccc aggaactcgg cggcctgggg
      721 caggtcgtag ccgaggaccg ccagcgtcag gtgctctgcc gccgggtaga cgtcgaaggc
      781 gtaccggtat ccgagggcat cgaagctggt gcgaccaccc tcggccggac cgacgttctg
      841 ctgggtcgtc ccggtgatgg ggaccagctc gtcggcagca gccacgatgt tcatgaacgg
      901 cacgttccgg gcgttctcca gccacaggtt gctgagcgtc tcggctccgc cggtgggggc
      961 accggcgccg acccagatcc cgtcaccggg cgggcccacg acggtgaccg cgctgccgaa
     1021 caggtcgggg tagagcgtgc cgagccggta ggtggcgtag ccgcccatcg agtacccggt
     1081 gatggcggtc gaatcggggt cgagggtgtg gtggcgggcg atgtcgttcc acatctcgaa
     1141 cacgtcgtac tcggcttcgt gctggtacca cccgtcctcg ccccgggcgt cagcggtggc
     1201 gacgaggctt ccgcgctcct cccccagctg ctggatgccg gtggagccgt tgtactgcca
     1261 gtggcgctcg ccgagggagt gcaggtccag cgtgagcggc gcgggcgtgc ccggctggta
     1321 ccccgagggc acgtacagcg agtacgcctg gagctggccg agcgtggcgg ggaagctgct
     1381 gtcatcgcgc ccctcgccca gctggaggcg gctggggaag aaccggacca tggtgccgtg
     1441 ctcaggcacc gtggtccccg actgccgggc agcgatggca tcgaagtcga tgtcgtgggc
     1501 gtaccgggtc ggctccttgg cagcgagggc ggccgactgg tcctcgtcgg gcggcgcgct
     1561 gctggtgagg atggcctcgt cgaagcggaa gccgaggttg aagatcgccg ccttgccatc
     1621 gccgccgccg gggtcggtct cggtggcggt cgcctgcggg gcgaggaatc cgccggcgtc
     1681 ggggtcgtgc aggccgacgg ccagggtcgc ccgccaggtg ccggtcggct gggccacggc
     1741 gtcgggcacg gccacggtga tctggttggc ctcgaggtcc acggtcgagg tgaggtcctg
     1801 acgctgccat gccgcaccgt cccaggcgct ccacgaggcc ccggttcccc acgcgctcag
     1861 cactgcatcg gtgcccggga acggggcgcc gggttcgtcg ggcaggtcgg ccgacccggt
     1921 ggctgcgtcc cggtcggtgt cgaaggcgac ggcgacgtgg gtggtgtcct cggccagcag
     1981 ggtgttgagc gtgaaccggt agtgggtggc gccgtccaac cggtccgaga tgcggagctc
     2041 gaccaggtcg gcggcgttgt cggcgaagcg cccgcgatcg gtcgggtagc tgagcccgtc
     2101 gccgctgtcg tcgtagatgt ggtcggtgta gaggtactcg ccgtccacgt agcggtcggt
     2161 gccggacacc cggtcgaacg gggcgacgaa ggccgaatgg cggttctcca gctgcggggc
     2221 gaccggtgcg gggtcgtaga ggacgtctgg cccgggccgc tcaccgctgg ccacctgggg
     2281 cagcgatgcc agggtggcct ggtcggtggg cggcgcacct tcctcggtcg agctggccag
     2341 cggtgtcagc gctccgaaca tggcgacggc agcgatgccg accaacgcgc gacgtgtggt
     2401 gctcttcatg tcgatcccct cgctccgatc ccaggccgtt ccctcggccc tgaggtcgct
     2461 tcgacggggg cgacgccgaa ccctgccaac tcatcgggcc ctcggccggg tacgactggt
     2521 gcgaccatgt tcgcccgcct ccctcccctc gcgcgcctgg cgctcgtctc cctcgtcctc
     2581 atcgcgctgg cgtgggcgat cttcggtcga tcggtcgagg acgagatcgc ggtggtgccg
     2641 gacacgacgg acaccaccga accgtccgtc ttcgagccgc tcgacaccag cgacaccggg
     2701 gtcgtcctcg tcgacgacct ggtgctcccc gtcaccggcg gcgaggacgg cgcctggacc
     2761 gtcctgacgc cgtgcgccaa cgccgcgacg gtcgacggcg agctgctcac cggcgcccac
     2821 gtcgtcatcg atcccggcca cggcggacgg gagaccggcg ccatcggccc gagcggggtc
     2881 gtcgaggccg aaatcaacct cgacgtcgcg ctgaaggcga aggccctcct ggaggcggag
     2941 ggggccacgg tggcgctgac ccgcaacgac gacacccggg tcaccctcga gacccgcgcc
     3001 gccatcgccc gggcgctcga cccgctgctg ttcatctcga tccaccacaa cggcggcccc
     3061 acccgcaccg ccgagcgacc gggcgtgcag gtgtaccacc agcaccagac gccggagtcg
     3121 gcccggttcg ccggagtgct gttcgagcag cttcaggagg cgatggcgcc cttctccgag
     3181 acgtggtccg gcggcaacgc caccggcgtg cgcgcccgcc tcggcgagga cggcgacgac
     3241 ttctacggcg tgctccgcgc ctccgagggc gtcccggccg tgctcgccga ggcgctgtac
     3301 atgtccgacg agcccgaggc caccctcctg ctgcgtgacg acgtgcgcca ggccgaggcc
     3361 caggccatca ccgacgcgat cgtcgcctgg ctcgactctc cctacgccgg
//
LOCUS       DATISU010000144         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_751710, whole genome shotgun sequence.
ACCESSION   DATISU010000144 DATISU010000000
VERSION     DATISU010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751710"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VLR27_05955"
     CDS             complement(<1..1157)
                     /locus_tag="VLR27_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HSP03025.1"
                     /translation="MLIANRGEIAVRVIRACKELGVTSVAVYSDLDRDSLHVRLADEA
                     YALGGTTAAESYLNTEKILEIIEQSGAEAVHPGYGFFSENPDFAREITDKGVVFIGPK
                     PEAMIVMGEKVSSRIAAQEAGVAGVPGTTEYLSSGDEVVAFGEEHGWPVAIKAAYGGG
                     GRGMRVVRSADEAHDALESAQSEALKGFGRDECYVERYLTWPRHVEMQIIADSHGNCV
                     WIGERDCSAQRRHQKLVEESPAPNFPAEIRQAMGEAAVKVAKACGYINAGTVEFLYQD
                     GDFFFLEMNTRLQVEHPVTEMVSGIDLVAEQLRVAAGEPLSFTQDEISLRGHSIELRV
                     NAEDPAEGKFLPSPGPIATLVPPQGNGVRWDGGYDSGDEVSQYYDNLIGKLV"
     gene            1187..>2004
                     /locus_tag="VLR27_05960"
     CDS             1187..>2004
                     /locus_tag="VLR27_05960"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HSP03026.1"
                     /translation="MKGDDAICGPGTRPRSRFTDVRHVTETGSTNTDVMELARQGEPE
                     GVVLVADHQTAGRGRAGRTWTAPPGASLLCTILLRPPAPVAPLATFALAVAASEAVEQ
                     VAGFRPGLKWPNDLVVEDTDADGGVRTRKLAGILAEAEWSPGSHIAAGYRPPKAHERA
                     TVAAGIGLNVAWPDEVPDDLADIAVAINHVSEARPSRDEVLDALLERLVVHYGALVDG
                     GVDQVLDAWRARSTTLGRRVRIDLGADDLVGTAVDVTADGHLVVEPLEGGRRTIA"
BASE COUNT          279 a          696 c          735 g          294 t
ORIGIN      
        1 acgagcttgc cgatgaggtt gtcgtagtac tggctgacct cgtcaccgga gtcgtagccg
       61 ccgtcccagc gcacgccgtt gccctgcggc gggaccagcg tggcgatggg gccgggggag
      121 gggaggaact tgccctcggc cgggtcctcg gcgttgacgc gcaactcgat ggagtggccg
      181 cgcagggaga tctcgtcctg ggtgaaggac agcggctcgc cggcggcgac gcgcagctgc
      241 tcggcgacga ggtcgatgcc cgagaccatc tcggtgacgg ggtgctcgac ctggagccgg
      301 gtgttcatct cgaggaagaa gaagtcgccg tcctggtaca ggaactcgac ggtgccggcg
      361 ttgatgtagc cgcaggcctt ggcgaccttc acggcggcct cgcccatggc ttggcggatc
      421 tcggccggga agttgggggc gggggattcc tcgaccagct tctggtggcg gcgctgggcg
      481 gagcagtcgc gctcgccgat ccatacgcag ttgccgtggg agtccgcgat gatctgcatc
      541 tcgacgtggc ggggccaggt gaggtagcgc tcgacgtagc actcgtcgcg gccgaagccc
      601 ttcagcgcct cggactgcgc ggattcgagc gcgtcgtggg cctcgtcggc ggagcgcacg
      661 acacgcatgc cccggcctcc gccgccgtag gccgccttga tggccaccgg ccaaccgtgc
      721 tcctcgccga acgcgacgac ctcgtcgccc gagctcaggt actcggtggt gccgggcacg
      781 ccggccacac cggcctcctg tgcggcgatc cgggaggaga ccttctcacc catgacgatc
      841 atcgcctcgg gcttggggcc gatgaacacg acgcccttgt cggtgatctc ccgggcgaag
      901 tcggggttct ccgagaagaa gccgtagccg ggatgcacgg cctcggcgcc ggactgctcg
      961 atgatctcga ggatcttctc ggtgttcagg tacgactcgg ctgccgtcgt gcccccgagg
     1021 gcgtaggcct cgtcggcgag gcggacgtgg agcgagtcac ggtcgaggtc ggagtagacg
     1081 gccacggacg tgacgccgag ctccttgcac gcccggatga cccggacggc gatctcgcca
     1141 cggtttgcga tcagcacctt ggaaaacact ccgtagtgtt atcgcggtga agggtgatga
     1201 cgccatctgc gggcccggga cccggccccg ctcccggttc acggacgtgc gacacgtcac
     1261 ggagaccggg tcgaccaaca ccgacgtgat ggagctggcc cgccagggcg agcccgaggg
     1321 cgtggtgctc gtcgccgacc accagaccgc cggccgggga cgggccggac gcacctggac
     1381 cgcacctccc ggggcgtcgc tgctgtgcac gatcctgctg cgcccgccgg cgccggtggc
     1441 cccgctggcc accttcgccc tcgccgtcgc ggcctccgag gccgtggagc aggtcgcggg
     1501 gttcaggccc gggctcaagt ggcccaacga cctcgtggtc gaggacaccg acgccgatgg
     1561 cggcgtgcgc acccgcaagc tggccgggat cctggccgag gccgagtggt cgcccggctc
     1621 ccacatcgcc gccggctacc gcccgcccaa agcgcacgag cgggcgacgg tcgcggccgg
     1681 catcgggctg aacgtggcct ggcccgacga ggtgcccgac gacctggcgg acatcgccgt
     1741 ggccatcaac cacgtgagcg aggctcgtcc gagccgcgac gaggtgctgg acgcgctgct
     1801 cgagcgcctc gtcgtccact acggcgccct ggtcgacggc ggggtcgatc aggtgctcga
     1861 cgcctggcgg gctcgctcca ccaccctcgg ccgccgggtc cgcatcgacc tcggcgccga
     1921 cgacctggtc ggcaccgccg tggacgtcac cgccgacggg cacctggtgg tcgagccgct
     1981 ggagggcggc cggcgcacca tcgc
//
LOCUS       DATISU010000145        13249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_332638, whole genome shotgun sequence.
ACCESSION   DATISU010000145 DATISU010000000
VERSION     DATISU010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 13249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13249
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332638"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..906
                     /locus_tag="VLR27_05965"
     CDS             253..906
                     /locus_tag="VLR27_05965"
                     /inference="COORDINATES: protein motif:HMM:NF023511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HSP03027.1"
                     /translation="MSLLQDPRNDTVDEDRTVTNRSDRPLHADDGGTVDLTRDHVEAG
                     EGRATDAHDEADQHPEAHDERPVHGPVEVHGPVQIDPSGRVPWRGMRIRRVRLLSVAK
                     LSFIFWVLAFAVLLGTTVAVWTAARAFGFIGEIETTVVTALGIDAFEIDGGALFRIAA
                     AAVAFLTMLGWVMTILLAAVYNASCAVFGGLAVETGPLKRRKRVFSLRHRGFVTIRS"
     gene            918..2909
                     /locus_tag="VLR27_05970"
     CDS             918..2909
                     /locus_tag="VLR27_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HSP03028.1"
                     /translation="MTTIRHFLGRLGQLATLFTTVGVVGILAVVVLSASALGVAHVTR
                     ESVPELPDPGVDVSAIDDLQTAIDIELSRPSVVVDPDGVVVGRFADDERYEPFAVGDV
                     PEIVEQVLTAAEDEDFRDHNGFDPSGIARAMVRNASSGEIEEGGSTLTQQLAKNLFTG
                     NESDLDRKLQELQVAIDIEERFSKDEILTAYANTVFLGNGAHGFEAASKEYFDKPAAE
                     LSLSETALLVGILPAPSDRNPRTDFEEAEESRRTVLERVRETGAFTPAEVDAALDEVP
                     QVLPARPRFEGFAYYVDYVRRYLLDTVGMSPEELYQGGLRIETALDRELQYIARLSVD
                     KHIPQGAGPEAAVAVVDHRSGLVTAVIGGRSFDDAQVNLALGSGGAGTGRQPGSSFKP
                     FALAAALEQGFVPQQRIGAPQTYLPTTVLDPKPVHNFTQRGYGQLSLIDATVRSINTS
                     YVALTEAIGAPAVRDTATALGVNGLPDYVGPSIGIGSYEVAPLDMAIAYGGFAADGRR
                     VEAGPVTRILDSAGNVVHDLRPNVEDRPQAVSPFTARWVTEILRQNVERGTATRAGFG
                     RPAAAKTGTSNDYGNAWLVGYTPALSAAVWVGHPLGNISMVNTAGYARVTGGSIPALI
                     WHDVMSFAHRDIPPTPFAGPFPAPAPTKPLPGGPIGGQQ"
     gene            complement(2926..3285)
                     /locus_tag="VLR27_05975"
     CDS             complement(2926..3285)
                     /locus_tag="VLR27_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP03029.1"
                     /translation="MSAGRPESLSAVTLGVTDMDASCAFYDALGFETVHGGPGTPFTS
                     YAVGEGFLNLQLVARTEVGWGRFIVHVDDVDAMHERALAAGLRPTMAPSDAPWGERYF
                     HIDDPDGHEVSFARPLA"
     gene            complement(3282..6761)
                     /locus_tag="VLR27_05980"
     CDS             complement(3282..6761)
                     /locus_tag="VLR27_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSP03030.1"
                     /translation="MTDIVDQDARRRIAEDHATTLFVEAGAGSGKTSSLVSRVVSLVL
                     AGADVTSIAAITFTEAAAAELRARVRRTLEEVEAGGEVDWVEDSPEARKAAGAALDRL
                     DRATICTLHAFAQRLLLAAPIEARLPPAVEVHDDISSSLRSEERWRRFERQLLDDDAL
                     ADTMRMSLTLGISSQDLQAVADTLGQNWDLVEEARDAGLVEDDRAVAVDRSVLRVDDW
                     IARIDAIEEMLVACTDPDGDRLAQWVTGYAVPLREELREAASDPYELVLLATSGLKRP
                     AKNAGTKGCWPDGSKPAVIEAGCAVIDAIAADVAALTDQVLTRLGAEIALYTLDDADA
                     RRREGRLEFHDLLVLARQVLRSERSVRQRFHQRYQHLLIDEFQDTDPIQIELAVRIAA
                     DPDHDTAVPWHDVDVEEGRLFFVGDPKQSIYRFRRADIGLFSQTAARYTGGATSLRVN
                     FRTVGPVLAFCNAVFGGDDDGLIRHAVEPDTQATAQPHYVALEPHRPPIAGDPGPAVV
                     VLGDGDPHPSAEAARSAEAADVAAVLARAHREGWYVDAGGGPRPCRWSDMAILLPSRT
                     TLSHLRSCLDDAEIPYRLESGTLVYGTTEVADLLAILRAVDDPGDPIAAVGALRSPAY
                     GCGDDDLLRWTREHGRITYLGSGVRPAEALAALPPDEVGPVERGLADLRARNRAAPFQ
                     AVPEIVESVLRDRRLLELCLAGRRHRDLWRLYRIVVEHARQFADTEDGGLRAFLHWAD
                     LQRDDKLRATSPVLPEADVDAVRILTIHGAKGLEFGITAVSGLSAQDPNGRTGATVRF
                     ASDGGFEFKMRKGMETTRYQAVRTVDETMDEHERIRLLYVALTRARDHLIVSTHHTKS
                     AIGRSHGGRISTRLGALADELDDLAVRWTSADEDGPLPSGGHAPDAAGVVPSPVDWQA
                     EVDAFTRRRTATLARGGGGVVSATAIARNLAVVPADDGSLAEPATPEREPWRRGRAGT
                     SIGSAVHAVLQHADLTDPGGRVVPDLARWQTAVEGLPADAADLVAAKARAALASPLVR
                     RAVTSGQLHRELHVAAPMSAVLGQEGSEAHDVDLVEGFVDLCFDDGDGLVVVDYKTDD
                     VPSDAAVAAALERYAPQGAAYALLLEAATGRPVAEVHFLFLRGTEAVDAPVPDLAAAV
                     DRVRGQLRTEASA"
     gene            complement(6758..9916)
                     /locus_tag="VLR27_05985"
     CDS             complement(6758..9916)
                     /locus_tag="VLR27_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HSP03031.1"
                     /translation="MTCDAQWTAHGDGALAALHHAVDAARAGAPLAPVTVVTPSPSVA
                     VATRRALARRAGGSVGVGFHALGAVAEQLAAPLLAEAGLGAGVDRELVVTAVRVALGD
                     QPGRFGPIAEHRVTWERLAATVVEVDALGEGSLAQIRDAGPVPADVVRVRDAVRRRLG
                     PLGSDAVIGLAAQVARGGGPRVEALGTVIVHLPDRFTEAELDLLGAIGAHRPVQLLLG
                     ATGDRVLDEALAAHVAPLVPTTPPALAPPEITEAISANDIDDEVRAGVRRLLALADEG
                     VPLHRMALVHPSGPPYARVVADVLHAARVPFSGPSTRRLAQTVAGRVLLGVLEVDRSR
                     FGRQEVVDLWASGVVVDADGVPLPAASFDERTRWLGVIHDPARWRAALDAEETRMRAR
                     LDAAAEEDEDTGWIERRLELDARLRRAVDVLDELCTGLPTTWPEVAAWAADVLDRLCG
                     PVRSRGSWSEVELDADATVRLALARLAALEDLEPTPTPEVIRDTIRTVLDAPAPRQGR
                     TGTGLLVTTVDAPPLVPLDAVAVVGLAEGHLPRLAGDDALLGDELRRTVGLAAVDDQQ
                     RRQRRAFLASLASAGHHRLVTYARNDQRSGRALVPSRWLVEVVERHAQLRPDTEALMA
                     GERVPGVTLVASHGAGLVDVAEGRTAALHLEEHALASLLHTGAFEAHPAADDPVLLAG
                     TELLRSRRSESFTRFDGNLDGDGIDVTALGVLSPTSLETYATCPRRWFFSHALKLRSQ
                     DRPEEIERIDGRERGTLAHRVLERFFDEVIESDAAPAPGEAWSDAARERLTAICDEEC
                     DAAEARGITGHPRWWAHDRDEIHRVLQRTLTGDEEQRREFGTAPVAVELTFGRQGAPP
                     LVIDLGDGRSLQLAGQADRVDRVTDRDGRTRVVVWDYKYSKPHGFDRITKDEATGGDP
                     LAGGTKIQLVAYAMAAGAAVDLDVPDDAEIHANYWFLRPPHTNERRGYPVDAALRQRF
                     TRVLGVLADGIAAGRFPARPGEHAWHWGNFEHCAWCEFDSICPRDRDEEWERVRHEPS
                     LGPLRQLAEDGSASVLDMEDAR"
     gene            10015..10485
                     /locus_tag="VLR27_05990"
     CDS             10015..10485
                     /locus_tag="VLR27_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSP03032.1"
                     /translation="MQDAAEVVGTIVVGLDGSSNSHEAVVWTTRLARALDAEVVAVHA
                     LGLLDQLEPDGPMVPTQPHREEIAERAEGLWSAPLEEAGVRHRVVLHDGNPVDVVLNV
                     VDEVGADLVVLGSRGIGGSPALLLGSTSSQVAQRALCPVTIVPPSDRPARGGSR"
     gene            10488..12191
                     /locus_tag="VLR27_05995"
     CDS             10488..12191
                     /locus_tag="VLR27_05995"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HSP03033.1"
                     /translation="MGDALRDLAQEWWDGTMARNPTVATRLGDHRFDDAIEDPSPAGL
                     ADERAFAVRVAEQARAIPVDDLDEGERVTHALLLDSCEQAIESVDSDEIQLASDGFTG
                     YPVSLLRSAAQLRAPDETSAEAQLERLRKLPGALDAVVSQWLTGADRGWTPAVAAVRR
                     SKAMVEGYLTGDLADDPFVGLSLPEGWDGTEDWRARAAAVVTEEVRPAYERVAAVLAD
                     LAERGRGDDRPGLVHVPGGDALYRSKARRATTTELTPQEIHDIGMVELTEVLPPQWAE
                     VGGRALGITDRDELFAALRSDPAMRYETAEEILEVAEAAIARALAGAPDWFERLPTAG
                     CVVEAVPASLAPGLPAAYYSPPPSDGSRPGTYFANTHDATRRNRFEAEVIAFHEAVPG
                     HHLERASAAELEDVPTFQQRGGYMAYSEGWGLYSERLADEMGLYSSDTMRLGMLTADA
                     WRSTRLVVDTGIHALGWSRSEAIEFFATHVPLDPEEAAVEVDRYIGMPGQALSYKIGQ
                     REMLRLRDDARTRLGDRFRIQRFHTTVLRHGGVPLALLADLVGDWVEAELSAGPPSLT
                     A"
     gene            12188..>13249
                     /locus_tag="VLR27_06000"
     CDS             12188..>13249
                     /locus_tag="VLR27_06000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HSP03034.1"
                     /translation="MIPSPVLPGAFERGRNLRRLADEHFDVLVVGGGITGVGVALDAA
                     SRGLRTALVERGDFASGTSSKSSKLVHGGLRYLQNGDVRLVYEALRERQRLLRNAPHL
                     VRVMPFLIPMFGKGGVIHPKLEKALGSAMWMYDLTGGARIGKLHQRISKEETLAHMPT
                     LPADRVGASYLYYDAQADDARLTLTVARTAAELHGAVCVNHAPVVELTHQGGRATGAV
                     VEDTESGDRIEVRARTVVNAAGVWADDIRALDEGMHPHAIRPAKGIHLTVPWSLVRND
                     IAVIAPVPKDKRSIFVVPWPAPEGEDPAFTYIGTTDTDYDGPTEDPQCTREDVAYLLD
                     AMNHSTTTEITERLVVGTWA"
BASE COUNT         1781 a         4864 c         4888 g         1716 t
ORIGIN      
        1 acgcggaaga tgcgctcgcg ctggggcgtc agcttgcggc cggaggaccg gaaggcgtcg
       61 gcgaggtcgg acggggagcg catcggtctc ccaccatagg tcaaggattg ctcatcgaca
      121 atcgttgtcg atcatcaacg agggcaggtg ggcgaagggg acggctcggc accgcgcggc
      181 cccgtgatgt ctggcctcgg aggggctggg gtaggaccca aggcgcacgc cagccagcgg
      241 aagagaagcg acatgtcact gctccaggac ccccggaacg acacggtgga cgaggaccgg
      301 acggtcacca accggtccga tcgtcccctg cacgccgacg acggcgggac ggtcgacctg
      361 acccgtgacc acgtcgaggc cggcgagggc cgcgccaccg acgcccacga cgaggccgac
      421 cagcaccccg aggcccacga cgagcggccc gtgcacggac ccgtcgaggt ccacggcccc
      481 gtgcagatcg accccagcgg tcgcgtcccc tggcggggga tgcgcatccg gcgggtgcgg
      541 ctgctgtcgg tggccaagct gtcgttcatc ttctgggtgc tggccttcgc cgtgctgctc
      601 ggcaccaccg tggccgtctg gaccgccgcc cgggcgttcg gcttcatcgg cgagatcgag
      661 accaccgtgg tcaccgcact cggcatcgac gccttcgaga tcgacggcgg ggcgctgttc
      721 cgcatcgccg ccgccgccgt ggccttcctc acgatgctcg ggtgggtgat gaccatcctc
      781 ctcgccgccg tctacaacgc ctcctgcgcc gtcttcggcg gcctcgccgt ggagaccggc
      841 ccgctgaagc gccgtaagcg ggtcttctcg ctgcggcacc gcggcttcgt cacgatccgc
      901 tcctgaggga gggggtcgtg accaccatcc gccacttcct cggccgcctc ggccagctcg
      961 ccacactgtt caccaccgtc ggggtcgtgg ggatcctggc ggtcgtcgtc ctgtcggcct
     1021 ccgcgctcgg cgtcgcccac gtcacccgcg agtccgtccc cgagctgccc gaccccgggg
     1081 tcgacgtgtc ggccatcgac gacctgcaga ccgccatcga catcgagctg tcgcggccct
     1141 ccgtcgtcgt cgaccccgac ggcgtggtcg tcggccgctt cgccgacgac gagcgctacg
     1201 agcccttcgc cgtcggcgac gtccccgaga tcgtcgagca ggtcctcacc gctgccgagg
     1261 acgaggactt ccgtgaccac aacggcttcg accccagcgg catcgcccgc gccatggtcc
     1321 gcaacgcctc cagcggcgag atcgaggagg gcggctcgac gctcacccag cagctggcca
     1381 agaacctgtt caccggcaac gagagcgacc tcgaccgcaa gctgcaggag ctgcaggtcg
     1441 ccatcgacat cgaggagcgg ttcagcaagg acgagatcct caccgcgtac gccaacaccg
     1501 tcttcctcgg caacggtgcc cacggcttcg aggccgcctc gaaggagtac ttcgacaagc
     1561 cggctgcgga gctcagcctc agcgagaccg cgctgctggt cggcatcctg cccgcaccgt
     1621 cggaccgcaa cccgcgcacc gacttcgagg aggccgagga gtcacgccgg accgtcctcg
     1681 aacgggtgcg ggagaccggc gccttcaccc ccgccgaggt cgacgccgca ctcgacgagg
     1741 tcccccaggt cctcccggcc cgcccgcggt tcgagggctt cgcctactac gtggactacg
     1801 tccggcggta cctcctcgac accgtcggca tgagccccga ggagctgtac cagggcgggc
     1861 tgcgcatcga gaccgcgctg gaccgcgagc tgcagtacat cgcccggctc tcggtggaca
     1921 agcacatccc gcagggcgcc ggacccgaag cggccgtggc ggtcgtcgac caccgcagcg
     1981 gcctggtcac ggcggtcatc ggcggccggt ccttcgacga cgcccaggtc aacctggccc
     2041 tcggcagtgg cggtgccggc accgggcgcc agccagggtc gtccttcaag cccttcgccc
     2101 tcgccgccgc cctcgagcag ggcttcgtcc cccagcagcg catcggcgca ccgcagacgt
     2161 acctgcccac gacggtcctg gaccccaagc cggtgcacaa cttcacccag cgcggctacg
     2221 gccagctcag cctgatcgat gccacggtgc gctccatcaa cacctcctac gtcgcgctga
     2281 ccgaggccat cggcgccccg gccgtgcgcg acaccgccac ggcgctcggc gtgaacggcc
     2341 tgcccgacta cgtcgggccg agcatcggca tcggttccta cgaggtggct ccgctcgaca
     2401 tggccatcgc ctacggcggc ttcgccgccg acggccgacg cgtcgaggcc ggcccggtga
     2461 cccggatcct ggattcggcc gggaacgtcg tccacgacct gcggccgaac gtcgaggacc
     2521 gcccccaggc tgtgtcgccc ttcaccgctc gctgggtgac ggagatcctg cggcagaacg
     2581 tcgagcgggg caccgccacc cgcgccggct tcggccggcc cgccgccgcc aagaccggca
     2641 cgtcgaacga ctacggcaac gcgtggctcg tgggctacac gcccgccctg tccgccgccg
     2701 tctgggtcgg tcacccgctc ggcaacatct cgatggtgaa caccgctggc tacgcccggg
     2761 tcacgggcgg cagcatcccg gcgttgatct ggcatgacgt catgtccttc gcccaccggg
     2821 acatcccacc gacgccgttc gccggtccct tccccgcacc ggcgccgacc aagccgctcc
     2881 ccggcgggcc catcggcgga cagcagtgag tcgacgcccg ctggctcagg ccagcgggcg
     2941 agcgaagctg acctcgtggc cgtcgggatc gtcgatgtgg aagtaccgct cgccccacgg
     3001 ggcatcggac ggcgccatgg tcggccggag gccggcggcg agcgctcgct cgtgcatggc
     3061 gtcgacgtcg tcgacgtgga cgatgaagcg accccacccc acctcggtgc gggcgacgag
     3121 ctgcaggttg aggaacccct cgccgacggc gtagctggtg aacggcgtgc ccgggccgcc
     3181 gtggaccgtc tcgaagccga gggcgtcgta gaacgcgcat gacgcatcca tgtcggtgac
     3241 gccgagggtg acggcgctga gggactcggg gcgaccggcg ctcatgccga cgcctccgtg
     3301 cgcagctgac cccggacgcg gtcgacggcg gcggcgaggt ccggcaccgg cgcgtcgacc
     3361 gcttccgtgc cccggaggaa caggaagtgg acctcggcga cggggcgacc ggtggctgct
     3421 tccagcagga gggcgtaggc ggccccctgg ggtgcgtagc gctcgagcgc cgccgccact
     3481 gcagcgtcgg acgggacgtc gtcggtcttg tagtcgacga cgacgagccc gtcaccgtcg
     3541 tcgaagcaca ggtcgacgaa gccctcgacc aggtcgacgt cgtgcgcctc ggacccctcc
     3601 tggccgagca cggccgacat cggtgccgcc acgtgcagct cgcggtgcag ctggccggac
     3661 gtgacggccc ggcgcacgag cgggctggcg agggcggccc gggccttggc cgccaccagg
     3721 tcggccgcgt ccgccggcag gccctcgacc gcggtctgcc agcgagccag gtcgggcacc
     3781 acccgcccgc ccgggtcggt gaggtcggcg tgctggagca cggcgtggac cgcgctgccg
     3841 atgctcgtcc cggcccgtcc gcggcgccac ggctctcgct cgggggtcgc tggttcggcc
     3901 agcgacccgt cgtcggccgg gacgacggcc aggttccggg cgatggccgt ggccgacacg
     3961 acccctcccc caccccgggc gagggtcgcg gtgcgccgtc gggtgaaggc gtcgacctcg
     4021 gcctgccagt ccaccgggct ggggaccacc cctgccgcat cgggcgcgtg gcccccgctg
     4081 gggaggggcc cgtcctcgtc ggcggacgtc cagcggacgg cgaggtcatc gagttcgtcg
     4141 gccagcgcgc cgagccgggt cgagatgcga ccaccgtggc tgcgcccgat cgccgacttg
     4201 gtgtggtggg tgctgacgat gaggtggtca cgggcgcggg tgagggccac gtagagcaac
     4261 cggatccgct cgtgctcgtc catggtctcg tcgacggtgc gaacggcttg gtaccgggtg
     4321 gtctccatgc ccttgcgcat cttgaactcg aagccaccgt cggaggcgaa ccggaccgtc
     4381 gcgccggtgc ggccgttggg gtcctgggcc gagaggcccg agacggcggt gatgccgaac
     4441 tcgagtccct tggccccgtg gatcgtgagg atccgcacgg cgtcgacgtc ggcctcgggc
     4501 aggacggggc tggtggcccg gagcttgtcg tcgcgctgca ggtcggccca gtgcaggaac
     4561 gcacgcaggc cgccgtcctc ggtgtcggcg aactgccgcg cgtgctcgac cacgatccgg
     4621 tagaggcgcc acaggtcccg gtggcggcgg ccggcgaggc acagctcgag gagccgccgg
     4681 tcgcggagga cggactcgac gatctcgggg acggcctgga acggggcggc gcggttgcgg
     4741 gctcgcaggt cggccagccc gcgctcgacc ggaccgacct cgtcgggtgg cagcgcagcg
     4801 agggcctccg ccggccgaac gccggagccg aggtaggtga tgcggccgtg ctcccgcgtc
     4861 catcggagca ggtcgtcgtc gccgcagccg taggccgggg accgcagcgc acccacggcg
     4921 gcgatggggt cgccgggatc gtcgacggca cgcaggatcg ccagcaggtc ggcgacctcg
     4981 gtggtgccgt acaccagggt cccgctctcg aggcggtacg ggatctcggc gtcgtccagg
     5041 cagctccgca ggtgcgagag cgtggtgcgg ctgggcagga ggatggccat gtccgaccag
     5101 cggcacggcc gggggccgcc gccggcgtcg acgtaccagc cctctcggtg ggctcgggcc
     5161 agcaccgccg ccacgtcggc ggcttcggcg ctccgggcgg cttcggcgct ggggtggggg
     5221 tcgccgtcgc cgagcacgac cacggccggg ccggggtcgc cagcgatggg gggccggtgg
     5281 ggctcgaggg ccacgtagtg cggttgggcc gtggcctgcg tgtcgggctc gacggcgtgg
     5341 cggatgaggc cgtcgtcgtc cccgccgaag acggcgttgc agaaggccag gaccgggccc
     5401 acggtgcgga agttcacccg caggctggtg gcgccacccg tgtagcgggc ggcggtctgg
     5461 ctgaacaggc cgatgtcggc ccggcggaac cggtagatgg actgcttggg gtcaccgacg
     5521 aagaacaggc ggccctcctc cacgtcgacg tcgtgccacg ggacggcggt gtcgtggtcg
     5581 gggtcggcgg cgatgcgcac cgccagctcg atctggatcg ggtcggtgtc ctggaactcg
     5641 tcgatcagca ggtgctggta gcgctggtgg aaccgctggc gcacgctccg ctcgctgcgc
     5701 agcacctgtc gggccagaac cagcaggtcg tggaactcga ggcgtccctc gcgccggcgg
     5761 gcgtcggcat cgtccagggt gtagagggcg atctccgcgc cgagtcgggt gagcacctgg
     5821 tcggtgaggg cggcgacgtc tgccgcgatg gcgtcgatga cggcacaccc ggcctcgatg
     5881 acggccggct tggacccgtc gggccagcac cccttggtgc cggcgttctt ggccggtcgc
     5941 ttcaagcccg acgtggcgag caggaccagc tcgtaggggt ccgacgcggc ctcgcgcagc
     6001 tcctcgcgca gcggcacggc gtacccggtg acccactggg cgaggcgatc gccgtccggg
     6061 tcggtgcagg cgacgagcat ctcctcgatg gcgtcgatcc gggcgatcca gtcgtcgacc
     6121 cggagcaccg agcggtcgac ggcgacggcg cggtcgtcct cgacgagacc ggcgtcgcgg
     6181 gcctcctcga ccaggtccca gttctgcccg agggtgtcgg cgacggcctg caggtcctgg
     6241 gaggagatgc ccagggtgag cgacatccgc atcgtgtcgg cgagggcatc gtcgtcgagc
     6301 agctggcgct cgaaccggcg ccagcgctct tccgagcgca gcgacgagct gatgtcgtcg
     6361 tgcacctcga ccgccggcgg cagccgtgcc tcgatgggcg cggccagcag gaggcgctgg
     6421 gcgaaggcgt ggagcgtgca gatggtggcc cggtcgagcc ggtcgagggc ggcgcctgct
     6481 gccttgcggg cttcgggcga gtcctcgacc cagtcgacct ccccgccggc ctcgacctcc
     6541 tcgagggtgc gccggacgcg ggcgcgcaac tcggcggcgg cggcctcggt gaaggtgatg
     6601 gcggcgatgg aggtgacgtc ggcgccggcc agcacgaggg acacgacgcg gctcacgagc
     6661 gaggacgtct tgccggagcc ggccccggct tcgacgaaca gcgtggtggc gtggtcctcg
     6721 gcgatgcggc gccgggcgtc ctggtcgacg atgtcggtca tcgggcgtcc tccatgtcga
     6781 gcaccgaggc ggagccgtcc tcggcgagct ggcgcagcgg accgagggat ggctcgtgac
     6841 gaacccgctc ccactcctcg tcgcggtccc gcgggcagat cgagtcgaac tcgcaccagg
     6901 cgcagtgctc gaagttcccc cagtgccacg cgtgctcgcc gggacgcgcc gggaagcgac
     6961 ccgccgcgat gccgtcggcc agcacgccga ggacccgggt gaagcgctgc cggagcgcgg
     7021 cgtccaccgg gtagccccgt cgctcgttcg tgtgcggcgg ccgcaggaac cagtagttgg
     7081 cgtggatctc ggcatcgtcc gggacgtcga ggtcgacggc ggctcctgcc gccatcgcgt
     7141 aggcgacgag ctggatcttc gtgcccccgg cgagcgggtc gcctcccgtg gcctcgtcct
     7201 tggtgatgcg gtcgaacccg tggggcttgc tgtacttgta gtcccagacc acgacgcggg
     7261 tgcgcccgtc ccggtcggtg acccggtcga cgcggtcggc ctgcccggcc agctgcagcg
     7321 agcgcccgtc gcccaggtcg atgaccaggg gcggcgcgcc ctggcggccg aaggtgagct
     7381 cgaccgccac cggtgccgtc ccgaactcgc gacgctgctc ctcgtcgccg gtcagggtcc
     7441 gttggagcac ccggtggatc tcgtcgcggt cgtgcgccca ccagcgcggg tgtccggtga
     7501 tgccgcgcgc ctcggcggcg tcgcactcct cgtcgcagat cgccgtcagg cgctcgcgag
     7561 cggcatcgga ccacgcctcg cccggcgccg gggcggcatc ggactcgatc acctcgtcga
     7621 agaaccgctc gagcacccgg tgggccaggg tgccgcgctc gcgaccgtcg atccgctcga
     7681 tctcctcggg ccggtcctgc gaccgcagct tcagcgcgtg ggagaagaac cagcggcggg
     7741 gacaggtcgc gtaggtctcg agcgacgtcg gggagagcac cccgagggcg gtcacgtcga
     7801 tgccgtcgcc gtcgaggttg ccgtcgaagc gggtgaacga ctcggaccga cgggagcgga
     7861 gcagctcggt gccggcgagc aggaccggat cgtcggcggc agggtgggcc tcgaaggcgc
     7921 cggtgtgcag gagcgacgcg agggcgtgct cctccaggtg cagggccgcg gtgcgaccct
     7981 cggccacgtc gaccaggccg gcaccgtggc tggcgaccag ggtcaccccg gggacccgct
     8041 cccccgccat cagcgcctcg gtgtcggggc ggagctgggc gtggcgctcg acgacctcga
     8101 ccagccagcg gctgggcacc agggcgcgcc cggagcgctg gtcgttgcgg gcgtaggtga
     8161 ccagccggtg gtgcccggcc gaggccagcg acgccaggaa cgcccggcgc tgccggcgct
     8221 gctggtcgtc caccgcggcg agccccaccg tgcgccgcag ctcgtcgccc agcagcgcgt
     8281 cgtcgccggc gaggcggggc aggtgcccct cggccaggcc gacgaccgcc acggcatcga
     8341 gcgggaccag gggtggcgcg tcgacggtgg tgacgagcag gccggtgccc gtgcgcccct
     8401 gacggggggc cggtgcgtcg agcaccgtgc ggatggtgtc gcggatgacc tcgggggtgg
     8461 gcgtcggctc caggtcctcc agggcggcga gccgggcgag ggcgaggcgg acggtggcgt
     8521 cggcgtcgag ctcgacctcg gaccagctgc cccggctgcg aaccggaccg cacaggcggt
     8581 cgagcacgtc ggcggcccag gcggcgacct cgggccacgt cgtcggcagg ccggtgcaca
     8641 gctcgtcgag gacgtcgacg gcccggcgca gccgggcgtc gagctcgagg cggcgctcga
     8701 tccaaccggt gtcctcgtcc tcctcggctg cagcgtcgag gcgggcgcgc atccgggtct
     8761 cctcggcgtc gagggccgcc cgccagcggg ccggatcgtg gatgacgccc agccaccgcg
     8821 tccgctcgtc gaacgacgca gcggggaggg gaacgccgtc ggcgtcgacc acgaccccgc
     8881 tggcccacag gtcgacgacc tcctggcgac cgaaccggga gcggtcgacc tcgaggacgc
     8941 ccagcagcac ccgcccggcg acggtctggg ccaaccggcg ggtgctgggt ccggagaacg
     9001 ggacgcgggc cgcgtggagg acgtcggcga cgacgcgcgc gtagggcggg cccgacgggt
     9061 ggacgagcgc catccggtgc agcggcaccc cctcgtcggc gagggcgagc aggcggcgca
     9121 ccccggcccg gacctcgtcg tcgatgtcgt tggcggagat cgcctcggtg atctcggggg
     9181 gcgcgagcgc gggcggggtc gtgggcacga ggggtgcgac gtgggccgcg agggcctcgt
     9241 ccagcacgcg gtcgccggtg gcgccgagca ggagctggac gggacggtgc gccccgatgg
     9301 cgccgaggag gtcgagctcg gcctcggtga accggtcggg caggtggacg atgacggtgc
     9361 cgagcgcctc gacccggggg cctccgcctc gtgcgacctg cgcggccagc ccgatcaccg
     9421 catcggaccc caggggaccg agccgtcggc ggaccgcgtc gcgcacccgc accacgtcgg
     9481 ccggcaccgg gccggcgtcg cggatctggg cgaggctgcc ctcgccgagt gcatcgacct
     9541 cgaccacggt ggctgccaag cgctcccacg tgacccggtg ctcggcgatg ggcccgaacc
     9601 gacccggctg gtcgcccagc gcgacgcgca cggcggtgac gacgagctcg cggtcgaccc
     9661 cggccccgag gccggcctcg gccaggagcg gcgcagcgag ctgctcggcc acggcgccga
     9721 gggcgtggaa gccgaccccg accgaccccc cggcccggcg ggccagggcg cgtcgcgtgg
     9781 cgacggccac ggatggggac ggggtgacga cggtgaccgg agccaacggc gcaccggcgc
     9841 gagccgcgtc caccgcatgg tgcagcgcgg cgagggcgcc atcgccgtgt gcggtccact
     9901 gggcgtcgca ggtcatgagg cgaacgtacc cggggggtgt gacgagaacg gttcctctcc
     9961 gcggcgagcc ggggcaccca ccgcaccccc gcccggatca agcacactcg tggcatgcag
    10021 gacgcagctg aggtcgtcgg aacgatcgtc gtcggtctcg acgggtcgtc gaacagccac
    10081 gaggcggtgg tctggaccac ccgcctggcc cgtgcgctgg atgccgaggt cgtcgcggtg
    10141 cacgccctcg ggctcctcga ccagctggaa cccgatggtc cgatggtgcc cacccagccc
    10201 caccgcgagg agatcgccga gcgcgccgag gggctgtggt ccgcaccctt ggaggaggcg
    10261 ggcgtccgcc accgcgtcgt gctccacgac ggcaacccgg tcgacgtggt gctcaacgtc
    10321 gtcgacgagg tgggggccga cctcgtggtc ctcggcagcc ggggcatcgg cggctcgccc
    10381 gcgctgctcc tcggcagcac cagcagccag gtcgcccagc gggcgctctg cccggtcacc
    10441 atcgtcccgc cctccgaccg gccggcccgg ggcgggagca ggtagccgtg ggcgacgcgc
    10501 tgcgcgacct ggcccaggag tggtgggacg ggaccatggc ccggaacccc accgtcgcca
    10561 cccgcctcgg cgaccaccgc ttcgacgacg ccatcgagga tccgagcccg gcgggcctgg
    10621 ccgacgagcg agccttcgcc gtgcgggtcg ccgagcaggc ccgggccatc cccgtcgacg
    10681 acctcgacga gggcgaacgg gtcacccacg ccctgctcct ggacagctgc gagcaggcca
    10741 tcgagtcggt cgacagcgac gagatccagc tggcgagcga cggcttcacc gggtaccccg
    10801 tctcgctcct tcgctcggcc gcccagctcc gtgcgcccga cgagaccagc gccgaggccc
    10861 agctcgagcg cctgcggaag ctgccggggg ccctcgacgc cgtcgtgtcg cagtggctca
    10921 ccggcgcgga tcggggctgg acgccggcgg tggcggcggt gcgccgctcg aaggccatgg
    10981 tcgagggcta cctgaccggc gacctcgcgg acgatccctt cgtcggcctc tccctccccg
    11041 aagggtggga cggcaccgag gactggcggg cccgagcagc cgcggtggtc accgaggagg
    11101 tccgcccggc ctacgagcgg gtcgctgccg tcctcgccga cctcgccgag cgcggccggg
    11161 gcgacgaccg ccccggcctg gtccacgtgc ccggcggtga cgccctctac cggtccaagg
    11221 cgcgtcgggc gaccaccacc gagctgaccc cgcaggagat ccacgacatc gggatggtgg
    11281 agctgaccga ggtcctcccc ccgcagtggg ccgaggtcgg gggccgggcg ctcggcatca
    11341 ccgaccgcga cgagctgttc gcggcgctgc gctccgatcc ggccatgcgg tacgagacgg
    11401 ccgaggagat cctcgaggtc gccgaagccg cgatcgcccg ggcgctcgcc ggcgcgccgg
    11461 actggttcga gcgccttccg accgccgggt gcgtcgtcga agccgtcccg gcctccctcg
    11521 cccctgggct gcctgccgcc tactacagcc cgcctccgag cgatggctcc cggccgggca
    11581 cctacttcgc caacacccac gacgccacgc gccgcaaccg cttcgaggcc gaggtcatcg
    11641 cgttccacga ggccgtcccc ggccaccacc tcgagcgggc cagcgccgcc gagctcgagg
    11701 acgtcccgac cttccagcag cggggtggct acatggccta cagcgagggt tggggcctgt
    11761 actcggagcg gctcgccgac gagatgggcc tctactcctc ggacaccatg cgcctcggca
    11821 tgctcaccgc cgacgcgtgg cgctccaccc gcctggtggt cgacaccggg atccacgccc
    11881 tggggtggag ccgcagcgag gccatcgagt tcttcgccac ccacgtcccc ctcgatccgg
    11941 aggaggccgc cgtcgaggtc gaccgctaca tcggcatgcc cggccaggcc ctctcgtaca
    12001 agatcggcca gcgggagatg ctccggttgc gcgacgacgc ccgcacccgc ctcggcgacc
    12061 gcttccggat ccagcggttc cacacgacgg tcctgcgcca cggcggcgtg cccctcgccc
    12121 tgctcgccga cctggtgggc gactgggtcg aagccgagct gtcagccggg cctcctagcc
    12181 tgacggcgtg atcccctctc ccgtcctgcc cggcgccttc gagcgcggtc ggaacctgcg
    12241 tcgcctggcc gacgagcact tcgacgtcct cgtcgtcggt ggtggcatca ccggcgtggg
    12301 cgtcgccctc gacgcggcca gccgggggct gcgcaccgca ctcgtcgagc ggggcgactt
    12361 cgcgtcgggg acgtcgtcga agagctccaa gctcgtccac ggcggcctgc gctacctgca
    12421 gaacggcgat gtccgcctgg tctacgaggc cctgcgcgag cgccagcggc tgctgcgcaa
    12481 cgccccgcac ctggtccggg tcatgccctt cctcatcccg atgttcggca agggcggcgt
    12541 catccacccc aagctcgaga aggccctcgg gtcggccatg tggatgtacg acctcaccgg
    12601 cggggcccgc atcggcaagc tccaccagcg catctccaag gaggagacgc tggcccacat
    12661 gccgacgctg ccagccgatc gcgtcggcgc cagctacctc tactacgacg cccaggccga
    12721 cgacgcccgc ctcacgctca ccgtggcccg cacggcggcc gagctgcacg gtgcggtgtg
    12781 cgtgaaccac gcccccgtgg tcgagctgac ccaccagggt ggacgggcga ccggggccgt
    12841 cgtcgaggac accgagtccg gcgaccgcat cgaggtgcgg gctcgcaccg tggtcaacgc
    12901 ggccggcgtc tgggccgacg acatccgagc cctcgacgag ggcatgcacc cccatgccat
    12961 ccggccggcc aagggcatcc acctcaccgt gccgtggtcg ctggtgcgca acgacatcgc
    13021 cgtcatcgcg ccggtgccca aggacaagcg gtcgatcttc gtggtgccgt ggccggcgcc
    13081 cgagggcgag gatcccgcct tcacctacat cggcacgacg gacaccgact acgacggccc
    13141 gaccgaggac ccccagtgca cccgtgagga cgtcgcctac ctgctcgacg ccatgaacca
    13201 ctcaaccacg accgagatca ccgagcgcct cgtggtcggc acgtgggcc
//
LOCUS       DATISU010000146        14568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_332744, whole genome shotgun sequence.
ACCESSION   DATISU010000146 DATISU010000000
VERSION     DATISU010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 14568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14568
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332744"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..654
                     /locus_tag="VLR27_06005"
     CDS             106..654
                     /locus_tag="VLR27_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03035.1"
                     /translation="MAAPEHVPTKPNQDVRTYGSPPRRPHSWRADRPADFADDHRQPV
                     GDLLGSPGPDQGYALRLARLLEPELHLTEGEHAKDALAGIVAIGLKRASVLGRAPVMH
                     DLRIAATLFGYLDPAPDPELVALRRELFEELSHFHHYMELRGLVDMVHPAVLRQTPAQ
                     VSDAYRSDWRSQLELDGAHPGV"
     gene            complement(715..1203)
                     /locus_tag="VLR27_06010"
     CDS             complement(715..1203)
                     /locus_tag="VLR27_06010"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HSP03036.1"
                     /translation="MPGTDATMDQSTAPADADPGDETSVVVTAAAAVPPEQVWHTLVE
                     RTSDWWSTPYLAPDAEPMRLEASLGGRVWSGSRTADSGSGQLHGTIRAFDPPERLEVG
                     SVLVPGAYAGSITITVERTGLGSEVRVEQMARGRVDATVEERISTGWAQLLARLTDLA
                     ER"
     gene            1287..1943
                     /locus_tag="VLR27_06015"
     CDS             1287..1943
                     /locus_tag="VLR27_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HSP03037.1"
                     /translation="MSIGRALRLGVRTAVLVGVLAAVYLFVTFVQVWQATTDESHEPA
                     QAIIVLGAAQYDGRPSPVLEARLAHAVELYEAGVAPTIVVTGGKQVGDRFTEARAGYT
                     YLRNQGIPEDAILREEQGANTWSQLAAATVLLSQRGIADVVLVSDDYHAYRLERIAEE
                     LGLDAQVSPIDPGLSARGRVQRLSRETVAVAVGRVIGFRRLVNLDDQLSNVSSLRPVG
                     "
     gene            1962..2036
                     /locus_tag="VLR27_06020"
     tRNA            1962..2036
                     /locus_tag="VLR27_06020"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1995..1997,aa:Gln,seq:ctg)
     gene            2126..2201
                     /locus_tag="VLR27_06025"
     tRNA            2126..2201
                     /locus_tag="VLR27_06025"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2160..2162,aa:Glu,seq:ctc)
     gene            complement(2299..3321)
                     /locus_tag="VLR27_06030"
     CDS             complement(2299..3321)
                     /locus_tag="VLR27_06030"
                     /inference="COORDINATES: protein motif:HMM:NF041045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor RsbA family regulatory
                     protein"
                     /protein_id="HSP03038.1"
                     /translation="MKSIDTVARPGSPDSFVHEAFLYGSDDEALSVAVPFLRDAREHG
                     EAAIVDVDDHKADLIRNELGQHADGVEFVGGRWYTNPASAIRQYLQCFESLLADGSRT
                     IRKLAQLPPTAINGSWHAWKRYEAVINVAFAEHPVRGICLFDRATIPAEVVDDVLCTH
                     PRLTTPPGPHAANERYTHPRDWLSQLPHPPLHDAERTAPLVELVDVLPHDARAAIAPL
                     AVGLSDGDRDGLLTCASELVSNAILHGAPPVTIRAWRDHGSVIVTVDDCGSGIDDPFL
                     GLIPPTRQMGAGGLGLWIAHQLCSDVAVMRTPDGFRVRIVGGGTGHGQTRSAAARQHG
                     SWRGGP"
     gene            complement(3411..4112)
                     /locus_tag="VLR27_06035"
     CDS             complement(3411..4112)
                     /locus_tag="VLR27_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HSP03039.1"
                     /translation="MDDVGTRLATTFADVARALLSDNDLDSVLNRIAAVAVEEVGGCE
                     SAGIDLIEKQVVRCVAWTSETARQMGEIQNEAGEGPCLSAIREHETFRSDDLEHEDRW
                     PQFAQRARTEMDVRSMLGFRLFAEEDTMGALNLHSSKPDAFDDRALAIGSILAAHAGI
                     AMSWAREREYMATALENRDVIGQAKGLLMAQRNVDADGAFALLRAASQRLNIKLIQVA
                     ERLTDPAGPGIAFDE"
     gene            4417..6804
                     /locus_tag="VLR27_06040"
     CDS             4417..6804
                     /locus_tag="VLR27_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSP03040.1"
                     /translation="MSVILDGNGRITACSASWVAAAAEHGIEPGSVGVGADYLAACDA
                     DGAGSAAGAAVRAVLRGEHHQRTLDYECSGPDERRWFTMRVAQLVGPASGAMVTHLDI
                     TAVRAAELAVRGELHELWSVVDPATDYVFLVDEGGQVLGRTRDESASRQWPLVGQVAL
                     DFAHPDDQSTLLELFVGLLASPGGRRIGRFRARGPHDAVWRHFNVTAANMVDDPTVGG
                     IVVGITDVSRLVRASARTAMGTAVLHERDVIALTTDESGIITGINTGACDLIGVDAAS
                     AVGRDIGDLVTGSDEPALCFASDSDVEQSGELVVVRRDGTAAPITVRVRRLEDPVTGF
                     GGRSFLGLDRSQIREQHETLQRQRTQDPLTGLADQRAFRNHLRRLCRSLPPGWEAVVA
                     EVALDRFVDINALHGSRVGDAVLRQVGRRLEHAAGPDGFVARLYGDVFVIASGRGEDP
                     DAFGHRLQRATEEIHTPDLVVHLSASVGLRPVARGDCDDTRAVLSDASQASASAKDAG
                     GGVVRRYGEGLRRSMERRLLLRSELSMAIERDQLSVEFQPVVRLADGMMVSVEALVRW
                     RHPTYGTLSPDVFIPLAEQTPVIHDIGTFVLETACRHAARWHALRPQLPLTVAVNLSA
                     SQLADATLPHRLHAILAHHGLHPSAITLELTESMLVDLNRGRERLTQLKAVGVRIAMD
                     DFGTGYSSLSRLKDLPLDVIKFDRSFITGIGQSPNDAAIVRTVVSLADVLGLDVIAEG
                     VETERDARALLATGCSLGQGYLWSASLRPEAIDDALRRSFPAVRVDSLPPGDGSR"
     gene            complement(6832..7218)
                     /locus_tag="VLR27_06045"
     CDS             complement(6832..7218)
                     /locus_tag="VLR27_06045"
                     /inference="COORDINATES: protein motif:HMM:NF019473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HSP03041.1"
                     /translation="MTTEPSTAAATVDAFIAAICADDLDRALTMVSDDVVYDNVPVGA
                     VEGPDGIRQVLGGFLAPATGIDWVVHRQVADGGTVMNERTDRFEMGGSWHELPVMGVF
                     EVVDGRITLWRDYFDMAPLQSLLGGG"
     gene            7306..8070
                     /locus_tag="VLR27_06050"
     CDS             7306..8070
                     /locus_tag="VLR27_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSP03042.1"
                     /translation="MRQLDRSSPLPLWAQLLEDLRGRLDAGEFTDGFPTDLQLTTDYG
                     VSRHTAREAVRRLQSEGVVSRERGRGTFVRSPTFEQPTGAIYSLFRSIEARGLEQRSR
                     VLDRSVVTDGDVAARLDLPPDAELVRLERLRSADGHPLAHDTVWLPAEVARPLLEVDL
                     THTSLYDELQARCGVRPASGTEWIATELPGDADRELLGVASKQPVFRIRRRSCTADGQ
                     ALEWRETVIRGDRYTFVAQWSPSGAYETVLQATDLV"
     gene            complement(8147..10015)
                     /gene="typA"
                     /locus_tag="VLR27_06055"
     CDS             complement(8147..10015)
                     /gene="typA"
                     /locus_tag="VLR27_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HSP03043.1"
                     /translation="MALRADLRNVAIVAHVDHGKTTLVDKMLWQSGAFRENQDVADRV
                     MDSMDLEKEKGITILAKNTAIPYLPEGGELVKINIVDTPGHADFGGEVERALSMVDGI
                     LLLVDASEGPLPQTRFVLRKALQARLPVILVVNKVDRPDSRIDEVVHEVEELFLDLDA
                     DEHQIDFPIVYCVARDGRATMERPSDGEGIVEGATLRPLLDLLVDHIPAPEFDDEMPL
                     QALVTNLDSSPYLGRLAMCRVRNGHIRKGQTVMWCKADGTQERVKIGELFITENLDRV
                     PAESAGPGEIIAIAGIEEITIGETLADAEDPHPLPVITIDEPSLSMTVGVNTSPLAGQ
                     DGKKMTARLVKARLDAELIGNVSIRVLPTERPDTWEVQGRGELQLAVLVEMMRREGFE
                     LTVGKPQVLTREIDDKVHEPVDRVTVDVPDDYLGVVQQLLAVRKGRLEQIVNHGTGWV
                     RLDYLVPARGLIGFRTELLTESRGTALLHHVFERFEPWAGEIRARRNGSLVADRTGET
                     RSYSLLSLQERGQLFVGPGVAVYEGMVIGENARTEDMDVNPTKEKKQTNVRASSSDNF
                     EKLIPATVLSLEQALEHIAEDECVEVTPTYVRIRKVELDQNLRARSAKRAKTAAGG"
     gene            10126..11277
                     /locus_tag="VLR27_06060"
     CDS             10126..11277
                     /locus_tag="VLR27_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP03044.1"
                     /translation="MTTTGPRTTGPAVDDHRALLAAIVDATEQAVVSVDLDGVVTSWN
                     LGAQALYGWTADDILGRSYDVVLPEGASSTAVIDRIVAGGTVRAIDAPRVRRDGSIVM
                     VGETTAPVRDADGSVVGMAMIARDVTERLETEELLEHYRRELDLRNQHLERSNTDLAQ
                     FAYVASHDLSEPLRAVAGMVGLLARRYQGRLDDDADEFIDFAVDGCERMRTMISDLLD
                     YSRAGRTELAVDDVDLGALVDRAVEALGSQVEEAGAVVVHHGLPTVQGDRTQLVRVVQ
                     NLLSNAVKFRRPGQPVRVEVSARRLGGDDGWRIEVADDGIGIEEQYRERIFRMFQRLH
                     TADQHPGTGIGLSISERIVAQHGGSIGVGSNDRGGSTFWFTLPDRIEAR"
     gene            11274..11720
                     /locus_tag="VLR27_06065"
     CDS             11274..11720
                     /locus_tag="VLR27_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSP03045.1"
                     /translation="MTVHLMQILLVEDSAADVRLTQEALKDAKILNELHVARDGVEAM
                     EFLQRSGDRAGAPRPDIVILDLNLPRKDGKEVLAEMKGDPDLRSIPVAVLTTSDAEID
                     VLESYDLGANCFLTKPVDLDQFIKVVQAVEEFWLGMVRLPERQDTT"
     gene            11717..>14568
                     /locus_tag="VLR27_06070"
     CDS             11717..>14568
                     /locus_tag="VLR27_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSP03046.1"
                     /translation="MSDAIQQRLDVETTVVPPTVELGALSLLVVEDSVSDQRLILETM
                     VDAGADRDRVSCVATLRDARQALIERPVSCVLLDLSLPDATGLESVTLLSAAAPDTPV
                     VIVTGQAVGPMVYAAMAEGADEYVCKSDLEPGLLGDVVARAIQRRRGAVRHRHALASA
                     AAVFDSIASPIAVIDGSGRIIAVNDTWERAGAVAGADPNRTGVGVNYLAVCDHAAGRF
                     AEGAAAIADGIRGVLAGDLDTFTMDYPCPSRDEERWFSLRVNPSGEMGGGAVLIHLDV
                     SDLKAAERGALARDSRLFGALDSTSPIFALIGPDASVLHVSDLTRTLLGLSAGERIGE
                     NAFSLIEPTDRVLAMEVFARVATVPGSREHVSIRALDGEGRWRDLDLTVVNLLEDPRV
                     GAIAVTGSDVTDTRLHQIASRLENRLLARLPVAIGLTDDSGILVYWNDRAEALFGVTR
                     GEALGRNTADLGLQTSNQEVQDEIRRTLAGGGPWEGDLDGLRADGTVVPLHVVVERID
                     DPEIDFHGVVYASYDNTERDRLESELEFQAFHDPLTALPNRRLFVERLDAVLAEHEAS
                     GRTAAVTFIDLDDFKALNDRVGHTAGDLALQIIADRLRAVLRPGDLVARIGGDEFVVG
                     FADLQGPGGAVAIAQRVLQAVRAPFRIGHHSLHLSATIGVAMSQPGVPADGLLRNADA
                     AMYEAKGSGKDTVWLFDDALQQRNRDRREQADDLLRSLTAGEVHAHLQPQYCLHTGDL
                     IGFEALARRQWPDGRTSDTADFIELAEESGVIDQIDQRVLAEACAALAVFHVERPEGT
                     PTVSVNVSATQIADPEFPDLVRRLVEDAGFPPHLLCIEVVESALADEHAAAATLEEIK
                     RIGVEVAIDDFGTGYSSLSRLQRFDVDYLKIDRGFVAGMDDAGDDAAVVSAVIGLAHA
                     LGLRTVAEGVEDEAQRDRLHELGVDIGQGYLWSRAVP"
BASE COUNT         2081 a         5143 c         5119 g         2225 t
ORIGIN      
        1 tggttcacgg tcggggtgtc ggagaacgtg atcgaggcgt cctggaccgc cctgatcgac
       61 gccatcgtct acggcctgca gcgctcgtct gcctagcatc acgtcgtggc agcgcccgag
      121 cacgtcccga cgaagccgaa ccaggatgtc cgcacctacg ggtcgccgcc gcggcggccc
      181 cacagctggc gggccgaccg tccggccgac ttcgccgacg accaccgcca gcccgtcggc
      241 gacctgctgg gcagcccggg tccggaccag ggctacgccc tgcgcctcgc ccgcctgctc
      301 gagcccgagc tgcacctcac cgagggcgag cacgccaagg atgcgctggc cggcatcgtc
      361 gccatcggcc tgaagcgggc ctcggtgctg ggccgggcgc cggtcatgca cgacctgcgc
      421 atcgccgcca cactgttcgg ctacctcgac cccgcccccg accccgagct ggtcgccctg
      481 cgccgcgagc tgttcgagga gctgtcgcac ttccaccact acatggagct gcgcggcctg
      541 gtcgacatgg tgcaccccgc cgtgctgcgc cagaccccgg cccaggtcag cgacgcctac
      601 cggagcgact ggcgcagcca gctcgagctc gacggcgccc accccggcgt ctgaccgatg
      661 cacttctgcc cgacggtcgt ggcggccgcc tgagcgcagc ggtctgccgg gcgatcagcg
      721 ctcggcgagg tcggtgagcc gggccagcag ctgggcccag ccggtggaga tgcgctcctc
      781 gacggtggca tcgactcggc cgcgggccat ctgctcgacc cgcacctccg agcccagccc
      841 cgtgcgctcg acggtgatgg tgatggagcc ggcgtaggcg cccgggacga gcacgctgcc
      901 cacctcgagc cgctccggcg ggtcgaacgc ccggatggtg ccgtggagct ggccgctgcc
      961 gctgtctgcg gtccggctgc cggaccacac ccggccgccc aggctcgcct cgaggcgcat
     1021 cggctcggcg tcgggggcga ggtagggggt ggaccaccag tcgctcgtcc gctcgaccag
     1081 cgtgtgccac acctgctcgg gcggcaccgc ggcggccgca gtgaccacca ccgaggtctc
     1141 gtcgcccgga tcggcgtcgg cgggggcggt gctctggtcc atcgtcgcgt cggtgccggg
     1201 cacccggcca gggtggcacg ccgggccgga caggtcgggg accggcgtgc gcgaccccgc
     1261 ccgggaccgt ctgtacgctc gccgccgtgt cgatcggccg tgcgctccgt ctcggcgtcc
     1321 ggaccgccgt gctcgtgggc gtcctggccg cggtgtacct gttcgtgacg ttcgtgcagg
     1381 tgtggcaggc cacgacggac gagtcccatg agccggccca ggccatcatc gtgctcgggg
     1441 ccgcccagta cgacgggcgc ccgtcgccgg tgctcgaggc ccgcctcgct cacgccgtgg
     1501 agctctacga ggccggggtg gcgcccacca tcgtggtcac cggcggcaag caggtgggcg
     1561 atcgcttcac cgaggccagg gccggttaca cgtacctgcg caaccagggc atccccgagg
     1621 acgcgatcct gcgggaggag cagggggcga acacctggtc ccagctggcc gccgcgacgg
     1681 tgctcctgtc acagcgcggg atcgccgacg tggtgctcgt cagcgacgac taccacgcct
     1741 accggctcga gcggatcgcc gaggagctgg gcctggacgc ccaggtctcc ccgatcgacc
     1801 ccggtctctc ggcccgcgga cgggtccaac ggctctcccg ggagaccgtc gccgtggccg
     1861 tcggccgggt catcggcttc cgccgcctgg tgaacctcga cgaccagctg tcgaatgtgt
     1921 cttcgctcag gcccgtcgga tagcgtggtc cgccccaccg atcgggcgtg gtgtaattgg
     1981 caacacagct ggttctggtc cagccattgg gggttcaagt cctcccgccc gaaccaagcg
     2041 ccccacggac cccggtcctg gcggcgcatc ccggcccgcc cgggctttgg tgcgcacacc
     2101 ggtgacggta tgctcgcccg ttcctgcccc gttcgtctag cggcctagga caccggcctc
     2161 tcacgccggc aacacgggtt cgaatcccgt acggggtgcc acgcgcgcag acgcccagct
     2221 cgatcgagcc gggcgtcttc cgcgttccgg gccgtcgacg gaggcggtgg gcggtccgag
     2281 cggagggtcg ccggtgcatc aaggaccgcc ccgccagctc ccatgctgac gggctgctgc
     2341 cgaacgggtc tggccgtgac cagtcccacc tccgacgatg cgcacccgga agccgtcggg
     2401 ggtgcgcatg accgcgacgt cactgcagag ctggtgggcg atccagaggc cgaggccgcc
     2461 cgctcccatc tggcgggtcg gtgggatcaa gcccaggaac ggatcgtcga tgccggaccc
     2521 gcagtcatcg accgtcacga tgacggagcc gtgatccctc cacgcgcgaa tggtcaccgg
     2581 gggtgcgccg tggaggatcg cgttcgagac gagctcgctg gcgcaggtga gcaggccgtc
     2641 tcggtcgccg tccgacagac ccacagcgag cggggcgatc gcggccctgg cgtcgtgtgg
     2701 cagcacgtcg acgagctcca cgagcggagc ggtgcgttcg gcatcgtgga gcggtggatg
     2761 cggcagctgc gagagccagt cgcgagggtg ggtgtagcgc tcgttcgcgg cgtgtggccc
     2821 cggtggcgtc gtgagacgcg ggtgggtgca cagcacgtcg tcgaccacct ctgccgggat
     2881 ggtggcacgg tcgaacaagc agatccctcg cacggggtgc tcggcgaagg cgacgttgat
     2941 caccgcttcg tagcgcttcc atgcgtgcca ggacccgttg atcgcggtcg gaggcagctg
     3001 tgcgagcttg cggatggtgc gactgccgtc cgccaggagc gactcgaagc attggaggta
     3061 ctggcggatg gcagacgcgg ggttcgtgta ccagcgcccg ccgacgaact cgaccccgtc
     3121 tgcgtgttgg ccgagctcgt tgcggatcag gtccgccttg tgatcgtcca cgtcgacgat
     3181 cgcggcttct ccgtgttcgc gggcgtcgcg gaggaacggg acggcgacgc tgagggcttc
     3241 gtcgtcggac ccgtagagga aggcctcgtg gacgaagctg tcgggggatc cgggccgcgc
     3301 cacggtgtcg atcgatttca tcaggaggac cttccagtcg agcggagggc ccctgtgaca
     3361 tcaaggactg ctggtttcga acctagaggg tcttcctgcc gcttccacgg ctactcgtcg
     3421 aaagcgatcc ccgggccggc ggggtcggtc agccgctcgg cgacctggat gagcttgatg
     3481 ttcagccgtt gcgacgccgc acggagcagc gcgaacgcgc cgtcggcgtc gacgttccgc
     3541 tgggccatga gcagtccctt ggcctggccg atcacgtcgc ggttctcgag ggccgtcgcc
     3601 atgtactccc gctcacgggc ccaggacatg gcgatgcctg cgtgcgcggc caggatggaa
     3661 ccgatcgcaa gtgcacgatc gtcgaatgcg tccggcttcg acgagtggag gttgagcgct
     3721 cccatcgtgt cctcctcggc gaagagacgg aacccgagca tggatcgcac gtccatctcc
     3781 gtgcgggcgc gctgcgcgaa ctgcggccac cggtcctcgt gctcgaggtc gtcggagcgg
     3841 aacgtctcgt gctctcgtat cgcggacagg cagggccctt cacccgcctc gttctggatc
     3901 tcgcccatct gccgcgccgt ctcgctcgtc caggcgacgc atcgcacgac ctgcttctcg
     3961 atcagatcga tcccggcgct ctcgcaacca ccgacctctt ccaccgcgac cgctgcgatc
     4021 ctgttgagga cgctgtcaag gtcgttgtcg gacaggagcg ctcgagcaac atcggcgaac
     4081 gtggtcgcaa gccgtgtccc cacgtcatcc atcgaccgag gctacccgtg ctccgatcga
     4141 gacggtggcg gtggagggag ggaggtacgg gggaggtggt ccgctaccgt gaccattgac
     4201 aacgtagtcc ttgatgtcac agggaccctc cgcaccggaa ggttcccgat gacgtcgact
     4261 gaccccacca cccctcactc ccgcgaggcg ggacacgatc gtccggcggg cttccccgtc
     4321 gtcggtgaga cgggcccgag ctggaacgac tggttcttcg catcggcttc ggtcggccgt
     4381 tgcgagcccg atgccacgct ggatcgctcg gacaccatgt ccgtcatcct ggacggcaac
     4441 ggccgaatca cggcgtgcag cgcctcatgg gtcgcagccg ccgccgagca cggcatcgaa
     4501 cccgggtccg tgggggtggg ggccgactac ctcgcggcgt gcgatgccga cggagcaggg
     4561 agcgcagcag gcgctgcggt gcgtgccgtg ctccgcgggg agcaccacca gcgcaccctg
     4621 gactacgaat gctcgggccc cgacgagcgt cgctggttca cgatgcgggt cgcccagctg
     4681 gtcggtcccg cctccggtgc gatggtcacg cacctggaca tcaccgccgt ccgagcggcg
     4741 gagctcgccg tgcgcggcga gctccacgag ctgtggagcg tcgtcgatcc ggcaacggac
     4801 tacgtgttcc tcgtcgatga gggcggtcag gttctgggcc gcacccggga cgagtcggcc
     4861 agccggcaat ggccgctcgt cgggcaggtt gcgctcgact tcgcgcaccc agatgatcag
     4921 tccacgctgc tcgagctgtt cgtggggctc ctggcctcgc ccggtgggcg gcgaatcggc
     4981 aggttccgtg ctcgtggccc gcacgacgcg gtgtggcgcc acttcaacgt gaccgccgcg
     5041 aacatggtgg acgaccccac cgtcggcggc atcgtcgtcg gcatcaccga cgtctcccgg
     5101 ctcgtccggg ccagcgcgcg gacagcgatg ggcaccgccg tgctccacga acgtgacgtc
     5161 atcgccctca cgaccgacga gagcgggatc atcaccggga tcaacaccgg cgcctgcgat
     5221 ctgatcgggg tcgacgcagc cagtgccgtc ggccgtgaca tcggtgacct ggtcaccggc
     5281 agcgacgagc cagctctctg cttcgcatcg gacagcgacg tcgagcagtc cggtgagctc
     5341 gttgtcgtcc gccgagacgg cacggccgcg ccgatcaccg tgcgggtgcg tcgactggag
     5401 gacccggtga cggggttcgg tggccgttcg ttcctcggtc tcgaccgatc gcagatccga
     5461 gagcaacacg agacgctgca gcgccagcgg acccaggatc cgctcaccgg tctggccgac
     5521 cagagggcgt tccgcaacca cctgcggcgg ctgtgccggt cgctgcctcc aggttgggag
     5581 gctgtggtgg ccgaggtggc gctggatcgc ttcgtagaca tcaacgccct gcacggctcc
     5641 agggtgggag atgccgttct gcggcaggtg ggacggaggt tggagcacgc ggcaggaccg
     5701 gacgggttcg ttgcccggtt gtacggtgac gtgttcgtca tcgcatcggg tcgcggtgag
     5761 gaccctgatg ctttcgggca ccgattgcag cgcgcgaccg aagagatcca cacgcccgac
     5821 ctggtcgtcc atctgtcagc cagcgtcggc ctccggccgg tggcacgtgg cgattgcgat
     5881 gacacccggg ccgtcctcag cgacgcctcc caggcctcag cgtcggcgaa ggacgccggg
     5941 ggcggtgtgg tccgccgcta cggcgagggc ctgcgccggt cgatggaacg tcgactgctc
     6001 ctacgaagcg agctgtccat ggccatcgag cgcgaccagc tctcggtgga gttccagccg
     6061 gtggtccgac tcgcggacgg gatgatggtg agcgtcgagg cgctcgtgcg gtggcggcat
     6121 ccgacctacg gcaccctttc ccccgacgtc ttcatcccgc ttgccgagca gaccccggtg
     6181 atccacgaca tcggcacctt cgtgctcgag acggcgtgcc ggcacgcagc acgatggcac
     6241 gcgctccgcc cgcagctccc cctcaccgtc gcagtcaacc tctcggcgtc gcagctggct
     6301 gatgccacgc ttccacaccg cctccacgcc atcctggcgc accacggact ccatccctcg
     6361 gcgatcacgt tggagctcac cgagtcgatg ctggtcgacc tgaaccgcgg gcgggagcgc
     6421 ctgacgcagc tcaaggcggt cggtgtccga atcgccatgg acgacttcgg gacgggctac
     6481 agctcgttga gccggctcaa ggacctcccg ctcgacgtca tcaagttcga ccgttcgttc
     6541 atcaccggga tcggtcagag ccccaacgac gccgcgatcg tgcgcaccgt cgtctccctg
     6601 gcagacgtgc tcgggctcga tgtcatcgcc gaaggcgtcg agaccgaacg agatgcccga
     6661 gcactccttg cgacgggctg ctcgctcggc cagggatacc tgtggtccgc gtcgctccgc
     6721 ccagaggcga tcgacgatgc gctccgacgc tcgttccctg cggtccgagt cgacagcctg
     6781 ccgccaggcg acggcagtcg atgacggggg ccgccagcgt ccggggccgg gtcagccgcc
     6841 gcccagcagg gactgcagcg gcgccatgtc gaagtagtcg cgccacaggg tgatgcgccc
     6901 gtcgacgacc tcgaacacgc ccatcacggg cagctcgtgc cagctgccgc ccatctcgaa
     6961 gcggtcggtg cgctcgttca tcacggtgcc gccgtcggcg acctggcggt gcaccaccca
     7021 gtcgatgccg gtggcagggg cgaggaagcc gcccagcacc tggcggatgc cgtcggggcc
     7081 ctccactgcg cccaccggca cgttgtcgta gacgacgtcg tcgctgacca tggtgagggc
     7141 gcggtcgagg tcgtcggcgc agatggcggc gatgaaggca tcgacggtgg ctgcagcggt
     7201 gctcggttcg gtggtcacgg gtcctccggg tcgcaggtct ggatggcacg gagttgacca
     7261 catcgccggt tgcgcgggcc gccggacgtc cgtacgttca gacacgtgcg ccagctcgac
     7321 cggtccagcc ccctaccgct ctgggcccag ctcctcgagg acctgcgcgg tcggctcgac
     7381 gccggcgagt tcaccgacgg cttccccacc gatctgcagc tcaccaccga ctacggcgtg
     7441 tcccgccaca ccgcccggga agccgtccgc cgcctccagt ccgaaggcgt ggtcagccgc
     7501 gagcggggtc ggggcacctt cgtgcgctcg cccaccttcg agcagcccac cggggccatc
     7561 tacagcctgt tccgctccat cgaggcccgg ggcctcgagc agcgcagcag ggtgctcgac
     7621 cgctccgtgg tcaccgacgg cgacgtcgcc gcgcggttgg acctgccccc cgacgccgag
     7681 ctggtccgcc tcgagcggct gcgcagcgcc gacgggcacc cgctggccca cgacaccgtc
     7741 tggttgcccg ccgaggtggc ccgtcccctc ctcgaggtgg acctcaccca cacctcgctc
     7801 tacgacgagc tgcaggcccg ctgcggggtc cgtcccgcct cgggcaccga gtggatcgcc
     7861 accgagctgc ccggagatgc cgatcgggag ctgctcggcg tcgcgtcgaa gcaaccggtg
     7921 ttccgcatcc gtcgccgctc gtgcaccgcc gacggacagg cgctggagtg gcgcgagacc
     7981 gtcatccggg gcgatcgcta caccttcgtg gcccagtggt cgccctcggg cgcctacgag
     8041 accgtcctac aggccaccga cctcgtctga tccgccccgc gagcccgttc tggcctacat
     8101 ccgctcccgc atgcgcggtc ggatgtaggc cagaacggat cgagggtcag ccgccggcgg
     8161 cggtcttggc ccgcttggcg gagcgggccc gcaggttctg gtccagctcg accttgcgga
     8221 tccgcacgta ggtcggggtg acctcgacgc actcgtcctc ggcgatgtgc tcgagcgcct
     8281 gctcgagcga caggaccgtg gccgggatca gcttctcgaa gttgtccgag gaggaggcgc
     8341 ggacgttggt ctgcttcttc tccttggtcg ggttgacgtc catgtcctcg gtgcgggcgt
     8401 tctcgccgat gaccatgccc tcgtagaccg cgacaccggg cccgacgaag agctggccgc
     8461 gctcctggag gctgagcagc gagtagctgc gggtctcgcc ggtgcggtcg gcgacgaggg
     8521 agccgttgcg ccgggcccgg atctcgccgg cccagggctc gaagcgctcg aacacgtggt
     8581 gcagcagcgc cgtgccgcgg ctctcggtga gcagctcggt ccggaagccg atgaggcctc
     8641 gggcgggcac caggtagtcg aggcgcaccc agccggtgcc gtggttgacg atctgctcga
     8701 ggcggccctt gcgcacggcc agcagctgct ggacgacgcc gaggtagtcg tcgggcacgt
     8761 cgacggtgac ccgatcgacc ggctcgtgga ccttgtcgtc gatctcccgg gtgagcacct
     8821 ggggcttgcc gacggtgagc tcgaagccct cgcgccgcat catctcgacg agcacggcca
     8881 gctgcagctc gccgcgaccc tggacctccc aggtgtcggg gcgctcggtg ggcagcaccc
     8941 ggatcgacac gttgccgatg agctcggcgt cgaggcgggc cttcaccagg cgcgctgtca
     9001 tcttcttgcc gtcctggccg gcgagcggcg acgtgttcac accgacggtc atcgacaggc
     9061 tgggctcgtc gatggtgatg accggcagcg ggtgcgggtc ctcggcgtcg gcgagggtct
     9121 cgccgatggt gatctcctcg atgcctgcga tggcgatgat ctcgccgggc ccggcggact
     9181 cggccggcac gcggtcgagg ttctcggtga tgaacagctc gccgatcttc acccgctcct
     9241 gggtgccgtc ggccttgcac cacatgacgg tctggccctt gcggatgtgg ccgttgcgga
     9301 cgcggcacat ggcgaggcgg ccgaggtagg gggaggagtc caggttggtg acgagggcct
     9361 ggagcggcat ctcgtcgtcg aactcggggg cggggatgtg gtcgacgagc aggtcgagca
     9421 gcgggcgcag ggtggcgccc tcgacgatgc cctcgccgtc gctcgggcgc tccatcgtgg
     9481 cccgcccgtc ccgggcgacg cagtagacga tggggaagtc gatctggtgc tcgtcggcgt
     9541 cgaggtcgag gaagagctcc tccacctcgt gcacgacctc gtcgatgcgg gagtcgggcc
     9601 ggtcgacctt gttgaccacc aggatcaccg gcagacgggc ctgcagggcc ttgcgcagca
     9661 cgaagcgggt ctgggggagc ggaccctcgc tggcgtcgac cagcaggagg atgccgtcga
     9721 ccatcgacag ggcccgttcg acctcaccgc cgaagtcggc gtggccgggg gtgtcgacga
     9781 tgttgatctt gacgagctcg ccgccctcgg gcaggtaggg gatggccgtg ttcttggcca
     9841 ggatcgtgat ccccttctcc ttctcgaggt ccatggagtc catgacccgg tcggcgacgt
     9901 cctggttctc gcggaacgca ccggactgcc acagcatctt gtccaccagg gtggtcttgc
     9961 cgtggtcgac gtgggccacg atggccacgt tcctgagatc tgcgcgtagc gccactgact
    10021 gcctccgaag gcgggagagg ggggaacaca acagactaga atgaatcagt tggataccct
    10081 cctttggccc tgtcccggag gtcccggttg gggcacgatc gtgaagtgac caccactggg
    10141 ccccggacca ccggcccggc cgtcgacgac caccgcgcct tgctggctgc gatcgtcgac
    10201 gcgaccgagc aagccgtggt ctccgtcgac ctggacggcg tggtcaccag ctggaacctg
    10261 ggagcccagg ccctctacgg ctggaccgca gacgacatcc tcggccggtc ctacgacgtc
    10321 gtgctccctg agggcgcctc cagcaccgcc gtgatcgacc gcatcgtggc gggcgggacc
    10381 gtgcgcgcga tcgacgcccc tcgggtccgg cgcgacggca gcatcgtgat ggtgggggag
    10441 accacagcgc cggtgaggga cgccgatgga tcggtggtcg gcatggccat gatcgcccgg
    10501 gacgtcaccg agcggctcga gaccgaggag ctgctcgagc actaccgccg ggagctcgac
    10561 ctcaggaacc agcacctcga gcgctcgaac accgacctcg cgcagttcgc ctacgtcgcg
    10621 tcccacgacc tctccgaacc gctccgcgcc gtggccggca tggtcggcct gctcgcccgg
    10681 cgctaccagg gtcgcctcga cgacgatgcc gacgagttca tcgacttcgc cgtcgacggc
    10741 tgcgagcgca tgcgcaccat gatcagcgac ctgctcgact actcgcgggc gggacggacc
    10801 gagctggccg tggacgacgt cgacctcggc gccctggtcg atcgggcggt cgaggcactc
    10861 ggctcccagg tcgaggaggc cggcgccgtg gtggtccacc acgggctgcc gacggtccag
    10921 ggcgaccgca cccagctggt ccgggtggtc cagaacctgc tcagcaacgc cgtcaagttc
    10981 cgccgaccgg gccagccggt ccgggtcgag gtgtccgctc gccgcctggg cggagacgac
    11041 gggtggcgca tcgaggtcgc cgacgacggc atcggcatcg aggagcagta ccgggagcgc
    11101 atcttccgga tgttccagcg gctgcacacc gccgaccagc acccgggcac cgggatcggc
    11161 ctgtcgatct ccgagcgcat cgtggcgcag cacggtgggt cgatcggggt agggagcaac
    11221 gaccgagggg gctccacgtt ctggttcacc ctgcccgacc ggatcgaggc ccgatgaccg
    11281 tgcacctgat gcagatcctg ctggtggagg acagcgcagc agacgtccgg ctcacccagg
    11341 aggcgctcaa ggacgccaag atcctcaacg agctccacgt cgcccgcgac ggtgtcgagg
    11401 cgatggagtt cctgcagcgg agcggcgacc gcgccggcgc gccccgcccc gacatcgtga
    11461 tcctcgacct caacctgccc cgcaaggacg gcaaggaggt cctggccgag atgaagggcg
    11521 acccggacct gcgcagcatc ccggtcgccg tgctcaccac ctccgacgcc gagatcgacg
    11581 tgctcgagag ctacgacctc ggcgccaact gcttcctgac caagccggtg gacctcgacc
    11641 agttcatcaa ggtcgtgcag gccgtcgagg agttctggct cggtatggtg cgtctgcccg
    11701 agcgacagga cacgacgtga gcgacgccat ccagcagcgg ctggacgtcg agaccacggt
    11761 cgtcccaccc acagtggagc tcggcgccct gtcgttgctc gtggtcgagg acagcgtgtc
    11821 ggaccagcgg ctcatcctgg agacgatggt cgacgccggc gccgaccggg atcgggtgtc
    11881 ctgcgtggcg accctgcgcg atgctcgcca ggcgctcatc gagcggccgg tcagctgcgt
    11941 gctcctcgac ctgtccctcc ccgatgcaac gggcctcgag agcgtgacct tgctgtcggc
    12001 ggcggcgccc gacacacccg tggtgatcgt caccggtcag gcggtcgggc cgatggtgta
    12061 cgccgccatg gccgagggcg cggacgagta cgtgtgcaag tccgacctcg agcccggtct
    12121 cctcggtgac gtggtggcgc gcgccatcca gcggcgacgg ggtgcggtcc gccaccgcca
    12181 cgcgctggcc tcggccgccg cggtgttcga ctccatcgcc tcgccgatcg ccgtcatcga
    12241 cggcagcgga cggatcatcg cagtgaacga cacgtgggag cgcgccggcg cggtggccgg
    12301 tgccgacccc aaccgcaccg gggtcggcgt gaactacctc gccgtctgcg accacgcggc
    12361 gggtcggttc gccgaaggcg ccgccgccat cgccgacggc atccgcggcg tgctggccgg
    12421 tgacctcgac acgttcacca tggactaccc gtgcccgagc cgggacgagg agcggtggtt
    12481 cagcctgcgg gtgaacccgt ccggcgagat gggcggtggg gccgtcctca tccacctgga
    12541 cgtcagtgac ctgaaggcgg ccgaacgagg agccctggcc cgggactcgc ggctgttcgg
    12601 cgccctggac tcgacctccc cgatcttcgc gctcatcggt ccggacgcat cggtcctcca
    12661 cgtcagcgac ctgacccgca cgctcctcgg cctgtcggcg ggcgagcgca tcggggagaa
    12721 cgccttctcg ctcatcgaac ccaccgatcg cgtcctggcg atggaggtgt tcgcccgggt
    12781 cgcgacggtg ccgggcagcc gggagcacgt ctcgatccgt gccctcgacg gtgagggtcg
    12841 ctggcgggac ctggatctca ccgtggtcaa cctgctggag gacccccggg tcggggccat
    12901 cgccgtcacg ggcagcgacg tcaccgacac ccgcctgcac cagatcgcca gtcgcctcga
    12961 gaaccggttg ctcgcccgcc tccccgtggc catcggcctg accgacgaca gcggcatcct
    13021 cgtctactgg aacgatcggg ccgaggcgct gttcggggtc acccgcggcg aggcgctggg
    13081 gcgcaacacc gccgacctcg gcctccagac cagcaaccag gaggtgcagg acgagatccg
    13141 ccggaccctg gccggaggtg ggccgtggga gggcgacctc gacggtctgc gagccgacgg
    13201 cacggtggtc ccactgcacg tggtggtcga gcgcatcgac gatcccgaga tcgacttcca
    13261 cggcgtggtc tacgcctcct acgacaacac cgaacgggat cgtctcgagt ccgagctcga
    13321 gttccaggcg ttccacgacc cgctcaccgc gctgcccaac cgtcgcctgt tcgtcgagcg
    13381 tctcgatgcc gtgctggccg agcacgaggc atccgggcgc accgcggccg tgaccttcat
    13441 cgacctcgac gacttcaagg cgctcaacga ccgggtcggg cacacggctg gcgacctggc
    13501 gctgcagatc atcgccgacc ggctgcgggc ggtcctgcgc cccggcgacc tggtcgcccg
    13561 catcggaggc gacgagttcg tggtcggctt cgccgacctg cagggacccg ggggcgccgt
    13621 ggcgatcgcg caacgggtcc tgcaggccgt acgggcaccg ttccgcatcg gacaccacag
    13681 cctccacctg tccgccacca tcggcgtcgc catgtcgcag cccggcgtgc cggccgatgg
    13741 cctgctccgc aacgcggatg cggccatgta cgaggccaag ggcagcggca aggacacggt
    13801 gtggctgttc gacgacgcgc tccagcagcg caaccgcgac cgccgcgagc aggccgacga
    13861 cctgctccgc tcgctgacgg cgggggaggt ccacgcccac ctccagcccc agtactgcct
    13921 gcacacgggt gacctcatcg gcttcgaggc tctcgcccgg cggcagtggc cggacgggag
    13981 gacgtccgac acggccgact tcatcgagct cgccgaggag agcggcgtca tcgaccagat
    14041 cgaccagcgg gtgctggccg aggcgtgcgc ggcgctggcc gtgttccacg tcgagcgtcc
    14101 agagggcacg ccgacggtct ccgtcaacgt gtccgcgacc cagatcgccg acccggagtt
    14161 ccccgacctg gtgcgccggc tcgtcgagga cgcggggttc ccgccgcacc tcctgtgcat
    14221 cgaggtcgtg gagtcggcgc tggccgacga gcacgccgcg gcggccaccc tcgaggagat
    14281 caagcggatc ggcgtcgagg tcgccatcga cgacttcggc accggctact cgtcgttgag
    14341 ccgcctgcag cgcttcgacg tcgactacct gaagatcgac cgggggttcg ttgccggcat
    14401 ggacgacgcc ggggacgatg cggccgtcgt ctcggccgtg atcggtctcg cccacgcact
    14461 gggcctgcgc accgtcgccg agggcgtcga ggacgaggcc cagcgtgacc ggttgcacga
    14521 gctcggcgtc gacatcggcc aggggtacct gtggagccga gcggtgcc
//
LOCUS       DATISU010000147         1536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_751909, whole genome shotgun sequence.
ACCESSION   DATISU010000147 DATISU010000000
VERSION     DATISU010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751909"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1201
                     /locus_tag="VLR27_06075"
     CDS             167..1201
                     /locus_tag="VLR27_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP03047.1"
                     /translation="MADRMAQGTVRDEMDAIADATTTKPPRLRRRTVVLDDGHRVGLS
                     VCGEGVPLVMIHGVIAEGMLYARTLRRLAGAGFKVIAVDSAGHGRTAGLGRKGWGWEA
                     YIDLHEQVLDHLGIERAVFVGHSMGGRVVVELAARNPERAIAVIPVNAAIGGLWDQLT
                     GAARYVPFLFPLGIGLLVTDTAIGALQGRKQMGALARLAAPSLAQRVRELPSLPAAVF
                     ATATAKNSLDRLRSLADADVPVMVLHGDRDLTIWFTNARQAAEAAGGTLVRIEGGLHS
                     WLLEDADSLPAVIGSLLDGVLGDALTDRTPSIEACYGPEALALTIDRPVSKPYQLEPS
                     HRWRIEPLPH"
     gene            complement(1269..>1536)
                     /locus_tag="VLR27_06080"
     CDS             complement(1269..>1536)
                     /locus_tag="VLR27_06080"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HSP03048.1"
                     /translation="IGIVDYCHRTRPLVVEINGEVFHSSLTDRAADEQRYRRLVAAGY
                     EVLVVWEYDVFHQPHRIVRALEQIVAQPHAPRVIRPTNAPWEAW"
BASE COUNT          209 a          598 c          508 g          221 t
ORIGIN      
        1 ggatcgaccg ggagcgcctg gccgtgaacg tcgacagcgt ctaccgggag ctcgaccccg
       61 agcggggccg caccgacgtc gtcaccctgt tcgggatgat ggccgcgctc gccccctgac
      121 gtcgggtatc ggcctgtttc gcccggttgc tgggtacggt ccgtccgtgg ctgaccgcat
      181 ggcgcagggg accgtccgcg acgagatgga tgcgatcgct gacgcgacca ccaccaagcc
      241 accccgtctg cgacggcgga cggtcgtgct cgacgacggc caccgggtcg gcctgtcggt
      301 ctgcggcgag ggcgtcccgc tggtcatgat ccacggcgtc atcgccgagg ggatgctgta
      361 cgcccgcacg ctccggcggc tcgccggcgc cgggttcaag gtcatcgccg tcgacagcgc
      421 cggccacggt cgcaccgccg ggctgggccg caagggctgg ggctgggagg cctacatcga
      481 cctccacgag caggtgctcg accacctcgg catcgagcgc gcggtgttcg tcggccactc
      541 catgggtggc cgggtcgtgg tcgagctcgc cgcccgcaac cccgagcggg ccatcgctgt
      601 catcccggtg aacgccgcca tcggcggcct gtgggaccag ctcaccggcg ccgcccgcta
      661 cgtgccgttc ctgttcccgc tcggcatcgg cctgctggtg accgacaccg ccatcggcgc
      721 gctgcagggc cgcaagcaga tgggcgccct ggctcggctc gccgccccgt cgctggccca
      781 gcgggtgcgg gagctcccgt cgctgcccgc cgccgtgttc gccaccgcca cggccaagaa
      841 ctccctcgac cgcctgcgct ccctcgccga cgccgacgtc ccggtgatgg tcctgcacgg
      901 cgaccgggac ctcaccatct ggttcaccaa tgcccgccag gccgccgagg ccgccggcgg
      961 cacgctggtc cgcatcgagg gcggcctgca ctcctggctg ctcgaggacg ccgacagcct
     1021 ccccgccgtc atcggctccc tcctcgacgg cgtcctcggc gacgccctca ccgaccgcac
     1081 cccctcgatc gaggcctgct acggccccga ggccctcgcc ctcaccatcg accgcccggt
     1141 gtccaagccg taccagctcg agcccagcca ccgctggcgc atcgaacccc tcccccactg
     1201 accccccccc gcccgttctg gcctacatcc atgaccacca gatggcacgg atgtagtcca
     1261 gaacgaggtc accaggcctc ccacggtgcg ttcgtcggac ggatgacgcg aggggcgtgc
     1321 ggctgggcca cgatctgctc gagcgcacgc acgatccggt gcggctggtg gaagacgtcg
     1381 tactcccaca ccacgagcac ctcgtagccg gccgccacga gtcggcggta gcgctgctcg
     1441 tcagcagccc ggtcggtgag ggacgagtgg aaaacctcgc cgttgatctc cacgaccaac
     1501 ggtcgagtgc ggtggcagta gtcgacgatg ccgatg
//
LOCUS       DATISU010000148        15392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_658803, whole genome shotgun sequence.
ACCESSION   DATISU010000148 DATISU010000000
VERSION     DATISU010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 15392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15392
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658803"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2106
                     /locus_tag="VLR27_06085"
     CDS             <1..2106
                     /locus_tag="VLR27_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03049.1"
                     /translation="ASSFVGRDRDRDELARLSASWPLVTLVGPGGVGKTRLARTVAAD
                     IEHRFSAGIRSIELAVVDDGAQVAAVVADGLGLVVGADGPLAALEAVGDLDVLVLVDN
                     CEHVVDAVADLVGRLAAGGRRLRVIATSREPLAVDGEQLWPVDPLPVTGSAGGVQLFV
                     DRVRAVRPDLAPDAEDLGLIAGIVERLDGLPLAIEMAAAAARTMPLPMLHERLGDELD
                     LPPLRRRDLDERQRTLKALLDWSLRLLDDEARSMFASLSVFAGPVAASDVAHVSGVAS
                     PFEVLCRLVDLSLLQVDVTGEEPHFRMLRTVRQRACEAAGADLDVLRARHAAWLLDAV
                     TSADHRLRGPDERRAHERLERLVDDARAAVAWARDHDPICAVRLVGRLFLFAQSRLRD
                     EVLGWAAEIVEQAPATLSGPDGELALAAAGQRAINGGDLERARRLIERAMELNDTTAD
                     PLVFELLSDVLLFAGDTAPAREAARVGLASASATGDTHGVVACLTGLVLADAYAGDHQ
                     AAARAMAEFAEPPELAPSDRAWLHYVEGERLLDLDPEPALAALQRAITTADGVENAYL
                     AAVARVSYASVLSRTGDPVAARAPFADLIAHWRAHGDRTHLLTTLRNLVTVLDRLHMP
                     ERAAELLGAVLDDAVAPSFGAERAMLETLLDRQERTLGPTELARRLAAGRARTIDEAA
                     HLAHEWLDEPTPAGNGGRW"
     gene            2117..2473
                     /locus_tag="VLR27_06090"
     CDS             2117..2473
                     /locus_tag="VLR27_06090"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSP03050.1"
                     /translation="MPSEEGAGRDGWADLVPFPGEHRPDEDRRDVGLRELIGDILREE
                     RHRQERTLADVAEAAAVSLQYLSEVERGRKDVSSELLGAMHEALGLDLAEVLERAARR
                     LHVRAQGRGGLRMLAA"
     gene            complement(2423..3061)
                     /locus_tag="VLR27_06095"
     CDS             complement(2423..3061)
                     /locus_tag="VLR27_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HSP03051.1"
                     /translation="MSSYTIPSVIESTPKGERVSDIFSRLLSERIIFVGTPIDDGVSN
                     VVIAQMLHLAADSTAEVQLYLNSPGGSFSAVMAIYDTMQFVAPDVATLCVGQATATSA
                     ILLAAGAPGKRSLLPHARVVLHQPHTEGSRGSISDLALEAAEIARIRAQGEELLARHT
                     GRPVEQIRADTDRALVLTGRDAIAYGIADNLLESGGSGGEHAQPAAALRPDV"
     gene            complement(3058..3666)
                     /locus_tag="VLR27_06100"
     CDS             complement(3058..3666)
                     /locus_tag="VLR27_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HSP03052.1"
                     /translation="MTTISTSPGTVYDRLLDKRIVVLGEAVDDESANRVVAQLLLLST
                     DDPQSDICLHINSPGGSVMAGLAVYDTMQLIPNDVATVAVGFAASMGQILLCSGAPGK
                     RYALPSAQVVMHEGSAGLGGSAADVEIQADNLQATLDRMRGIIARHTGHSIEEVVRDV
                     GRDRWFDADQARDYGFVDHVVTSLDEIIPAAPTRRIGLGRQS"
     gene            3791..5176
                     /locus_tag="VLR27_06105"
     CDS             3791..5176
                     /locus_tag="VLR27_06105"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP03053.1"
                     /translation="MAGSPTTAGVASTTPVPAIDDPEARARLGAVVLAAWPLLLSILL
                     LMVGSGLQGALLGVRAERAAFNSTVTGLVLGLYYLGYVAGSAWVPAMVRAVGHIRVFA
                     SLASLASATVVIHGVWVSPIPWMFLRFITGVCVAGLFIVSESWLNDVSTSRTRGSLLA
                     VYNSVVTAGLAIGSLMLNLADIGGFVLFVIGSVMLSVAAVPVALAPHEAPPPREYEPR
                     SVRDVVRSAPLGITGATLSGFATGAALGFGAVYATRAGFGVTGASKFIAFLLVGAVAG
                     QFPLGRWSDRTDRRYVMAVAALLVTVGAGIGVGATLRESFVGALAAAFFIGAGSFSLY
                     GLSFAHVADYVEPAAMPATGARLLTFNGLGAAAGPFVASAAIGIIGPEGIFHVLGALT
                     LPFVVYVLYRLTRRAAVSDDRRAHYTPLATSATVGALDELMSEISGIEVTDERPPDRR
                     RRVPLPRSVRR"
     gene            5173..5886
                     /locus_tag="VLR27_06110"
     CDS             5173..5886
                     /locus_tag="VLR27_06110"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HSP03054.1"
                     /translation="MTTRRQAMRDGVTDVAPMLVGIIPFGLVAGAAPIAAGLDLGHAV
                     AMSLLLFAGASQLAMADVLGSDGGVAVAVVTALTINLRMLLYSASLAPTLSTERLGAR
                     LRASYLLVDQVYALSIIRWDGEDDRRLRLPYYFAIGILLWTSWQITTVIGALVGSSVP
                     DDVPLDFAVPLVFLVLLVPVLQRWPSLVAAAVGGTIAVAAAEMGAARLAILIGGVAGI
                     VAGTVAEGHQERRREVSRP"
     gene            5883..6197
                     /locus_tag="VLR27_06115"
     CDS             5883..6197
                     /locus_tag="VLR27_06115"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HSP03055.1"
                     /translation="MSGTWIAILLAGAGTYAMRASFLAFANRFVDLPPLAQRVLRQIP
                     PAVLAALVVPALVRPTGQLDLWQPRLLAGVVAALVSWRTRNVGLTLVVGMGLLVLLEQ
                     LT"
     gene            6243..7433
                     /locus_tag="VLR27_06120"
     CDS             6243..7433
                     /locus_tag="VLR27_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HSP03056.1"
                     /translation="MSTTIQPPVTIDLRDPEFWRTRQHEAWTWARANDPVHWDEGSQL
                     WAITRHADVQHVERHAELFSSLRTYRLNETPTESNMIASDDPRHLQQRRLVNRGFTPK
                     AVREQSPHFEAMIDSMIDPLTPAGRCEVVDAIAGQLPARFTCNLLGLPEEDWRQVKGW
                     SEKLMRIDSMAFDQEAMAGLMEVTMELWGMVQAKVPELRGCPAHGLLSTWSNAEIDGE
                     QLDVETVFHETGLFVAGGAETTRTAIAHGLRTFCDHNDQWELLHERPELIPSAVDEVI
                     RWVTPLNNMFRKATEDVELRGRTIKAGDRLALVYPSANRDEDVFEDPFTFDVTRSPNP
                     HIAFGYGTHFCLGANFARRELEILLGKLTREWTNLRVEDEIDVEPNIFAPAVRSFGLG
                     FDAR"
     gene            7445..7717
                     /locus_tag="VLR27_06125"
     CDS             7445..7717
                     /locus_tag="VLR27_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HSP03057.1"
                     /translation="MRPVHAAEPSTSPTPTITPDTTVQPTTGDGDHDRYAHICRKEDV
                     TRAYITGEAIEALCGKKWVPTRDPDRYPVCPACKEAIGKIRNAGEN"
     gene            complement(7787..9334)
                     /locus_tag="VLR27_06130"
     CDS             complement(7787..9334)
                     /locus_tag="VLR27_06130"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HSP03058.1"
                     /translation="MTLPTAASVTRNPSQEQLRDWVLANMSRVQVSEHGNISYTTMVT
                     ARLAPSTYFVSEQPTGKPTMPRADYDHWAEQQDAFIADQDMVLIEGYIGPDPDFRTPV
                     RLFIERKNSNIPAMQQQLYFPVDDEAAFEEEHAEAGFTVIYTPGLLAPGMPNDRLIAV
                     DLDHYVTRVFGSDYFGESKMGGLRMWNHLVHERGGLAMHSGAKVFPAEETRSGEEELA
                     LIIGLSGTGKTTTTFREVKGSLPVQDDFIGLMPDGTVHATENGCFAKTYGLDADDEPT
                     IHGGAIDPEAWLENTRIDPITRQIDFFDDRYTANGRVTFPLADIRHRDPRDLPKAKYL
                     FILNRNENIIPAVARLKPEQAAHYFMLGETKGTSAGGAAEAGKSLRVPGTNPFWFEDD
                     ASQANRLLEILEQHPLEVYLLNTGRVGGGADDDRSKKVTIDVSAAVQAGIVDGTIEWE
                     TDPDFGYDVAASIPGIDDDELLQPRRLYERQDRGDEYRQLVDQLRRERTEYLDGFPEL
                     DDRVRAA"
     gene            complement(9360..9878)
                     /locus_tag="VLR27_06135"
     CDS             complement(9360..9878)
                     /locus_tag="VLR27_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HSP03059.1"
                     /translation="MPVYALGDLNPTIADDAFVHPDAVVIGDVTIGSESTIWPGAVLR
                     GDSGPITIGDRTSIQDGSVIHTTEWAPTQIGDGCVIGHIVHLEGCIIEDDALVGSGSV
                     VLHKAVVRTGGLVGANAVCNGGFEVPTGAMALGVPAKLRHDSVDLEMIKHSAEFYVER
                     GHRYRRELRRID"
     gene            complement(9931..11481)
                     /locus_tag="VLR27_06140"
     CDS             complement(9931..11481)
                     /locus_tag="VLR27_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HSP03060.1"
                     /translation="MPGRSRDTELRFREPSRRWPSLSGHVGGAAMLFVAAGIVISAVV
                     ELGAGGDEAAALMAAAGIAAVTGFALWRGTEVPEKMTASATFVAVGSTWILASIVGAI
                     PYLLTGTFDTIDDALFESVSGFTGTGSTVLAPIEAAPRGVLFWRNLTQWYGGTGIVVL
                     AVAILPLLGVGGMDMLSAEAPGPTTDRLAPRISETARRLWQVYAGLTVAAIALLLAVG
                     MSPFDAVTHAFTAVSTGGLSPYDSSIAAFDSVAVELALVVLMLVSAASFTLHWRAVTG
                     RPRVYAESPLLRFYLGVFVVFTAAITALLWTQQGLGFGKSLRDATFNVASLLTSTGFG
                     TADYVQWVPGTQLLLLALMVSGGMAGSTSGGLKLVRARVLLVHAVRELRRIRHPRAVL
                     PVRLGREAMPERIVQRVIGYALLYVVFIGLGCLALAFLGAGLTEAAGAATSAMGNMGP
                     ALGEAGPASNYLYFSRPARGLLMVMMLAGRLEIFPVLYVIGRLTGTLPGGSMRRVVRR
                     TTALAPTS"
     gene            11562..12578
                     /locus_tag="VLR27_06145"
     CDS             11562..12578
                     /locus_tag="VLR27_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HSP03061.1"
                     /translation="MRSPLALLVALALLAAACGGDDDATAADSEGDGDGDTGGLVVVT
                     TVAPITSIAADVIGDLAQIRGVVPEGTNSHTYEPTPSVSEVLEGADVVFANGLQLEEP
                     TLALARDVAGDATIVELGDLIVSPDEYLYDFSFPEDGGKPNPHLWTDPMLAKGYARYI
                     AETMSDVDPDNAEAYEANRAEFDEIVDELDAALRTALDTVPEDNRKLVTYHDAYAYWA
                     QTYGWTVVGAVQPEDFGDPSPRAVADLIDQIRAEDVPAVFGSEVFPSPVLETIAEETG
                     ADYVADLRDDDLPGQPGDDDHSWMALMRSNYATIVEVLGGDPEALEQLELRRVGPDAA
                     EYPQ"
     gene            12600..13376
                     /locus_tag="VLR27_06150"
     CDS             12600..13376
                     /locus_tag="VLR27_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HSP03062.1"
                     /translation="MTPLLTLEGVAAGYGDGSVITGVDLQIDRGDFVGIVGPSGSGKS
                     TLLRVLLGSVAVSEGRLDRTGGTRLGYVPQVEQIDWDFPVTVAQCVLMSRTRGRILPW
                     ASKAEKADVARVLDRLGIGDLAGRHIRALSGGQQQRVFLARAMLAEPDLLLLDEPTSG
                     VDVRTRHEILHLLGELHDGGLGIVLTTHDLNGIAAHLPRLVCLNKQVIGAGAPHEVLV
                     PSVLEATYGSPMEVLEHGGMRVVVDVPSEAQVIQLRDRSA"
     gene            13373..14224
                     /locus_tag="VLR27_06155"
     CDS             13373..14224
                     /locus_tag="VLR27_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HSP03063.1"
                     /translation="MITLDELLRPFEFQFFQNGLVVATIAGALCGLIGVYVVLRSMSY
                     IGHGLSHAIFGGFAVSALAGVNVFLGAGLWGLASALAVSSVTRRRPIGSDAAIGVITT
                     ASFAFGLAVLQLGGGADVDADAALFGNVLGVTRGDVWIVSGVTLFAIAVIVLRYRSLL
                     FVTFDPEVAEASGVRVARIDALLMLVLAASILATMNVMGVTLIAAALVVPAVVARMVT
                     SSFSTMLWLSTLIGAVSGFVGMNLSYHLDVASGPTIVLVGGSFFLCALAVTGVRGRTR
                     LAGVQAH"
     gene            14245..>15392
                     /locus_tag="VLR27_06160"
     CDS             14245..>15392
                     /locus_tag="VLR27_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HSP03064.1"
                     /translation="MDSQRTKRMLESVLGVSVTGGNRIDVLKNGDQIFPAMLEAIDAA
                     ERSIDFLTFIYWNGEIGQEFADHLSAKAREGVRVRVLLDAAGAWELDTEVVDGMDEAG
                     CDVRWFRPIKARGALSANNRTHRKLLVCDEELGFTGGVGIADVWGGDARGEGEWRDTH
                     FRVVGPAVAGLRGAFFDNWAETGTDLVDDVDQFPELSQPGELDVQVIKGVAEAGNSDV
                     WNLFRVLIDCAEERIRITTAYFTPDEEILRRLLSASERGVEVEVLVPGPHADKRLVQV
                     ASEASFRELLDAGVQICTFQPSMLHAKVLTVDRAVCVVGSANMNQRSTEHDDEVCLVI
                     FDEAMTAVLDGHMDEDLQRAEPIDLDEWEKRGLLQRIEEKAVKVIDGLL"
BASE COUNT         2047 a         5576 c         5485 g         2284 t
ORIGIN      
        1 gcatcgtcgt tcgtcgggcg cgaccgggac cgtgacgagc tggcccggct gtcggcgagc
       61 tggccgctgg tcacgctcgt cggtccgggc ggcgtcggca agacccgcct cgcccgcacc
      121 gtggccgccg acatcgagca ccgcttctcc gccggcatcc gctcgatcga gctcgccgtt
      181 gtggacgacg gagcccaggt cgccgccgtg gtcgccgatg ggctcggtct cgtcgtcggc
      241 gccgacggtc cgctcgcggc cctggaggcg gtcggggacc tcgacgtgct ggtcctggtc
      301 gacaactgcg agcacgtcgt cgacgcggtg gccgacctcg tcgggcggct cgctgccggc
      361 gggcggcgac tgcgggtcat cgccacgagc cgtgaaccgc tcgccgtcga cggggagcag
      421 ctgtggccgg tcgacccgct cccggtgacg ggctcggcgg gcggcgtgca gctcttcgtc
      481 gaccgggtcc gggccgtccg ccccgacctc gctcccgatg ccgaggacct cggcctgatc
      541 gccgggatcg tggagcgcct cgacgggctc ccgctcgcca tcgagatggc ggcagcggcc
      601 gcacggacca tgccgctgcc catgctccac gagcgcctcg gggacgagct cgacctcccc
      661 ccgctcaggc ggcgcgacct ggacgagcgc cagcggacgc tgaaggcgct gctcgactgg
      721 tcgctgcggc tgctcgacga cgaggcccgg tcgatgttcg cctcgctgtc ggtgttcgcc
      781 gggccggtcg cggcgtccga cgtcgcccac gtctccggcg tcgcgtcgcc cttcgaggtc
      841 ctctgccgcc tggtcgacct ctcgctcctg caggtcgacg tgaccgggga ggagccgcac
      901 ttccggatgc tgcgcaccgt ccgccagcgc gcctgcgaag ccgccggcgc cgatctcgac
      961 gtgctccggg cgcggcatgc tgcgtggctc ctcgatgcgg tgacgtcggc ggatcaccgg
     1021 ctgcgcggtc cggacgagcg ccgggcccac gaacgcctcg agcgcctcgt cgacgatgcc
     1081 cgcgccgccg tggcctgggc gcgggaccac gacccgatct gcgccgtgcg gctcgtcggc
     1141 cggctcttcc tgttcgccca gtcgcgcctg cgagacgagg tcctcggctg ggcggccgag
     1201 atcgtggagc aggcacccgc gacgctctcc ggccccgacg gcgagctcgc cctcgccgcc
     1261 gccgggcagc gggccatcaa cggcggcgac ctggagcggg cgcgccggct catcgaacgg
     1321 gccatggagc tgaacgacac gaccgccgac ccgctggtct tcgagctgct ctccgacgtc
     1381 ctgctcttcg ccggcgacac cgcaccggcg cgggaggcgg cgcgcgtcgg gctggcgtcg
     1441 gcgtcggcca ccggggacac gcacggtgtc gtcgcgtgcc tcaccggctt ggtgctggcc
     1501 gatgcctacg ccggcgacca ccaggccgcg gcgcgagcga tggcggagtt cgccgaaccc
     1561 ccggagctgg ctccctccga ccgcgcctgg ctccactacg tcgaaggcga gcggctgctg
     1621 gacctcgatc ccgaaccggc cctcgccgcg ctccagcggg ccatcaccac cgccgacggg
     1681 gtcgagaacg cctacctcgc tgccgtggcc cgggtctcct acgcatccgt gctgtcacgg
     1741 accggcgatc cggtggcggc ccgggcgccc ttcgccgacc tcatcgccca ctggcgcgcc
     1801 cacggtgacc ggacccacct gctcaccacg ctgcgcaacc tggtcaccgt gctcgaccgg
     1861 ctccacatgc ccgagcgcgc tgctgagctg ctgggtgcgg tgctcgacga tgccgtggcg
     1921 cccagcttcg gcgcggagcg ggcgatgctc gagaccctgc tcgaccgcca ggagcgcacg
     1981 ctcggcccca ccgagctggc gcgccgcctg gccgccgggc gagcgcgcac gatcgacgag
     2041 gccgcgcacc tggcgcacga gtggttggac gaacccaccc cggcaggcaa cggcgggcgt
     2101 tggtagcgtc tgtgccatgc cgagcgaaga gggggccggt cgggacggat gggccgacct
     2161 cgtcccgttc ccgggtgagc accgccccga cgaggaccgt cgcgacgtcg gcctgcggga
     2221 gctgatcggc gacatcctgc gtgaggagcg gcatcgccag gagcgcacgc tcgccgacgt
     2281 cgccgaggcc gccgccgtct cgctgcagta cctctccgag gtcgagcgcg gtcgcaagga
     2341 cgtgtcgtcc gagctgctcg gtgcgatgca cgaggcgctc ggcctcgacc tcgcggaggt
     2401 cctggagcgc gcggcgcggc ggctacacgt ccgggcgcag ggccgcggcg ggctgcgcat
     2461 gctcgccgcc tgacccaccg ctctcgagca ggttgtcggc gatgccgtag gcgatggcgt
     2521 cgcggccggt gagcaccagc gcccggtcgg tgtcagcgcg gatctgctcc accggtcgac
     2581 cggtgtgccg ggccagcagc tcctcgccct gggcccggat ccgggcgatc tcggccgcct
     2641 cgagggcgag gtcgctgatc gagccgcggc tcccctcggt gtggggctgg tgcagcacga
     2701 cacgggcgtg cgggagcagc gaccgcttcc cgggcgcgcc ggcagcgagc aggatcgccg
     2761 aggtcgccgt cgcctggccg acgcacagcg tcgccacgtc cggggcgacg aactgcatcg
     2821 tgtcgtagat cgccatcacg gcgctgaacg agccgccggg cgagttgagg tagagctgca
     2881 cctccgccgt cgagtcggcg gcgaggtgca gcatctgggc gatgacgacg ttggagactc
     2941 cgtcgtcgat cggggtgccg acgaagatga tccgctccga gagcagccgg ctgaagatgt
     3001 cggagacccg ttcccccttg ggcgtggact cgatgacgct ggggatcgtg tagctgctca
     3061 cgactgcctc ccgaggccga tgcgcctggt cggcgccgcc gggatgatct cgtccagcga
     3121 cgtcaccacg tggtcgacga agccgtagtc cctcgcctgg tcggcgtcga accagcgatc
     3181 gcgcccgacg tcgcgcacca cctcctcgat cgagtggccg gtgtggcggg cgatgatccc
     3241 gcgcatccgg tcgagggtcg cctggaggtt gtcggcctgg atctcgacgt cggcggcgga
     3301 cccacccaac ccggccgatc cctcgtgcat cacgacctgg gcgctcggca gggcgtagcg
     3361 cttccccggc gcgcccgagc agagcaggat ctggcccatg ctggccgcga acccgacggc
     3421 gacggtcgcc acgtcgttgg ggatgagctg catcgtgtcg tagacggcga ggccggccat
     3481 gaccgacccg cccggcgagt tgatgtgcag gcagatgtcg ctctgcgggt cgtccgtgga
     3541 cagcagcagg agctgggcga cgacgcggtt ggcgctctcg tcgtccacgg cctcacccag
     3601 cacgacgatg cgcttgtcca gcaggcggtc gtacaccgtg cccggcgacg tggagatcgt
     3661 ggtcatgggg ccgagtgtgg cgtggtgatg tccgccgcgg tgccggttct gctgcaggca
     3721 gaacgggggt gaaccgccag cggacccggc agttgtcccg ccccgccggc gacgtgcacc
     3781 atgggccgtc gtggccggtt cccccaccac cgccggcgtc gcttcgacga cgcctgtccc
     3841 tgcgatcgac gatcccgagg ctcgggcgcg cctcggcgcg gtcgtgctgg ccgcgtggcc
     3901 gctgctgctg tcgatcctgc tcctgatggt cggcagcggc ctccagggtg cgctgctcgg
     3961 cgtgcgcgcc gaacgggccg ccttcaactc caccgtcacc ggcctcgtgc tcggcctcta
     4021 ctacctgggg tacgtggccg gctcggcgtg ggtgccggcg atggtccgcg ccgtgggcca
     4081 catccgggtc ttcgcctccc tggcctcgct cgcctcggcg accgtggtca tccacggcgt
     4141 gtgggtctcg ccgatcccgt ggatgttcct gcgcttcatc acgggcgtgt gcgtggctgg
     4201 cctgttcatc gtgagcgagt cctggctgaa cgacgtgtcc acgtcgcgca cccggggttc
     4261 gttgctcgcc gtctacaact ccgtggtcac cgccggcctc gccatcggca gcctcatgct
     4321 caacctcgcc gacatcggcg ggttcgtcct gttcgtgatc ggctcggtga tgctgtcggt
     4381 cgccgccgtg cccgtcgccc tggcaccgca cgaggcccca ccgccgcggg agtacgagcc
     4441 gcggtcggtg cgcgacgtgg tccggtcggc gccgctcggc atcaccggcg ccacgctgtc
     4501 ggggttcgcc accggcgccg ccctcggctt cggtgccgtg tacgccaccc gggctgggtt
     4561 cggcgtcacc ggtgcgtcga agttcatcgc cttcctgctc gtcggcgccg tggccgggca
     4621 gttcccgctc ggccggtggt cggaccggac cgaccgccgc tacgtgatgg cggtcgccgc
     4681 gctgctcgtc accgtcggtg ccggcatcgg cgtgggggcg acgctgcgcg agagcttcgt
     4741 cggtgcgctg gccgcggcgt tcttcatcgg cgccggctcg ttctcgctct acggcctgtc
     4801 gttcgcccac gtcgccgact acgtcgagcc cgccgccatg ccggccacgg gcgcccgcct
     4861 cctcaccttc aacggcctcg gtgccgccgc cggtcccttc gtcgcctccg ccgccatcgg
     4921 gatcatcggg cccgagggga tcttccacgt gctgggggcc ctgaccctgc ccttcgtcgt
     4981 ctacgtgctc taccgcctca cccgccgcgc cgcggtgagc gacgaccggc gcgcccacta
     5041 cacgccgctc gccacctcgg ccacggtcgg tgcgctcgac gagctcatgt cggagatcag
     5101 cggcatcgag gtgaccgacg agcggccgcc ggaccgccgc cggcgcgtgc cgctgccccg
     5161 ctcggtccgg aggtgaccac ccgccgacag gccatgcgcg acggcgtcac ggacgtcgcg
     5221 ccgatgctgg tcggcatcat cccgttcggc ctcgtcgccg gcgctgcacc gatcgccgcc
     5281 ggcctcgacc tcggccacgc cgtcgcgatg tcgctgctgc tgttcgccgg ggccagccag
     5341 ctggcgatgg ccgacgtgct cggcagcgac ggcggcgtcg cggtcgccgt ggtgaccgcc
     5401 ctgaccatca acctgcgcat gctgctgtac tcggcgtcgc tcgcgccgac gctgtcgacc
     5461 gagcgcctcg gtgctcgcct gcgcgccagc tacctcctgg tggaccaggt gtacgcgctc
     5521 tcgatcatcc gctgggacgg cgaggacgac cgccgcctgc gcctgccgta ctacttcgcc
     5581 atcggcatcc tgctctggac gtcgtggcag atcaccacgg tgatcggcgc actggtgggc
     5641 agcagcgtgc ccgacgacgt gccgctggac ttcgccgtcc cgctggtgtt cctggtgctg
     5701 ctcgtcccgg tgctgcagcg gtggccgagc ctcgtcgccg ccgcggtcgg cgggacgatc
     5761 gccgtcgcag ccgctgagat gggcgcggcg cggctcgcca tcctcatcgg cggcgtggcc
     5821 ggcatcgtgg ccgggaccgt cgccgagggc caccaggagc gccggcgcga ggtgtcgcgg
     5881 ccatgagcgg gacctggatc gccatcctgt tggccggggc gggcacctac gccatgcgcg
     5941 cctcgttcct cgcgttcgcc aaccggttcg tggacctccc cccgctcgcg cagcgggtcc
     6001 tgcgccagat cccgccggcg gtgctggccg ccctcgtcgt gccggccctc gtccgcccca
     6061 ccgggcagct cgacctctgg cagccccggc tcctcgccgg cgtggtcgcc gcgctcgtgt
     6121 cgtggcggac ccgcaacgtg gggctcaccc tcgtcgtggg catgggcctg ctggtgctgc
     6181 tcgagcagct cacctgagcc gccctcctgc aacgggcgtt gccaacttgg caggatgggc
     6241 tggtgagcac gacgatccag ccccccgtca ccatcgacct gcgcgatccg gagttctggc
     6301 ggacccggca gcacgaggcg tggacctggg cgcgggccaa cgacccggtg cactgggacg
     6361 agggatccca gctgtgggcg atcacccggc acgccgacgt ccagcacgtg gagcgccacg
     6421 ccgagctgtt ctcctcgctg cgcacgtacc ggctcaacga gacgccgacc gagtccaaca
     6481 tgatcgccag cgacgacccc cggcacctcc agcagcgccg gctcgtgaac cgcgggttca
     6541 cgccgaaggc ggtgcgggag cagtcccccc acttcgaggc gatgatcgac tcgatgatcg
     6601 acccgctcac gccggccggc cgctgcgagg tcgtcgacgc catcgccggc cagctgccgg
     6661 cccgcttcac ctgcaacctg ctcggcctcc ccgaggagga ctggcgccag gtgaagggct
     6721 ggtccgagaa gctcatgcgc atcgactcca tggccttcga ccaggaggcc atggccggcc
     6781 tcatggaggt gaccatggag ctgtggggga tggtgcaggc caaggtcccc gagctgcggg
     6841 gctgcccggc gcacgggctg ctgtcgacct ggtccaacgc cgagatcgac ggcgagcagc
     6901 tcgacgtcga gaccgtcttc cacgagaccg ggctgttcgt ggccggcggg gccgagacca
     6961 cccgcacggc catcgcccac gggctgcgga cgttctgcga ccacaacgac cagtgggagc
     7021 tgctccacga gcgccccgag ctcatcccct cggcggtgga cgaggtcatc cgctgggtca
     7081 cgccgctcaa caacatgttc cgcaaggcca ccgaggacgt cgagctgcgg ggccgcacca
     7141 tcaaggccgg cgaccggctc gccctcgtct acccgtcggc gaaccgcgac gaggacgtgt
     7201 tcgaggaccc cttcaccttc gacgtcaccc gctcgccgaa cccccacatc gccttcggct
     7261 acggcaccca cttctgcctc ggcgccaact tcgcccgccg cgagctcgag atcctgctgg
     7321 gcaagctcac ccgcgagtgg accaacctgc gggtcgagga cgagatcgac gtggagccca
     7381 acatcttcgc cccggcggtg cgatcgttcg gactcggctt cgacgcccgg tagcgtcgat
     7441 ggggatgcgc cccgtccacg ctgccgagcc gagcacgtcg ccgacgccca cgatcacgcc
     7501 ggacaccacc gtccagccga ccacgggcga cggcgaccac gaccgctacg cccacatctg
     7561 ccgcaaggaa gacgtgacgc gggcctacat cacgggcgag gccatcgagg ccctctgcgg
     7621 caagaagtgg gtgcccacgc gggacccgga ccgctacccg gtctgcccgg cgtgcaagga
     7681 agccatcggc aagatccgca acgccggcga gaactagcgc cggcccgggg ctgcaggccg
     7741 ttccgcagcc gctgccagaa gcttctggca gcgaccacgg aacacgtcag gcggcgcgga
     7801 cccgatcgtc gagctcgggg aagccatcga ggtactccgt gcgctcccga cggagctggt
     7861 cgaccagctg gcggtactcg tcgccccggt cctgccgctc gtacaggcgt cggggctgga
     7921 gcagctcgtc gtcgtcgatg cccgggatgg aggcggccac gtcgtagccg aagtccggat
     7981 cggtctccca ctcgatcgtg ccgtcgacga tgccggcctg cacggcggcc gacacgtcga
     8041 tcgtgacctt cttcgagcgg tcgtcgtcgg cgccgccacc cacccggccg gtgttgagca
     8101 ggtacacctc gagggggtgc tgctcgagga tctcgagcag ccggttggcc tgggaggcgt
     8161 cgtcctcgaa ccagaacggg ttggtgcccg gcacccgcag gctcttgccc gcctcggccg
     8221 cgccgccggc gctggtgccc ttggtctcgc cgagcatgaa gtagtgggcg gcctgctcgg
     8281 gcttgaggcg ggccacggcc gggatgatgt tctcgttccg gttcaggatg aacaggtact
     8341 tcgccttcgg caggtcccgg ggatcgcggt ggcggatgtc ggcgaggggg aaggtgaccc
     8401 gcccgttcgc cgtgtagcgg tcgtcgaaga agtcgatctg gcgggtgatc gggtcgatcc
     8461 gggtgttctc cagccacgcc tcggggtcga tcgccccgcc gtggatggtg ggctcgtcgt
     8521 cggcgtccag gccgtaggtc ttggcgaagc agccgttctc ggtggcgtgc acggtgccgt
     8581 cgggcatgag gccgatgaag tcgtcctgca cgggcaggga gcccttcacc tcgcggaacg
     8641 tggtggtcgt cttgccggtg ccggacagcc cgatgatcag cgccagctcc tcctcgcccg
     8701 atcgggtctc ctccgccggg aacaccttgg cgcccgagtg catggccagg ccgccgcgct
     8761 cgtggacgag gtggttccac atgcgcagac cgcccatctt ggactcgccg aagtagtcgg
     8821 acccgaacac ccgggtcacg tagtggtcga ggtcgacggc gatcagccgg tcgttcggca
     8881 tgcccggcgc cagcaggccc ggcgtgtaga tcacggtgaa cccggcctcg gcgtgctcct
     8941 cctcgaaggc ggcctcgtcg tcgaccggga agtagagctg ctgctgcatc gccgggatgt
     9001 tgctgttctt gcgctcgatg aacaggcgga ccggcgtgcg gaagtcgggg tcggggccga
     9061 tgtagccctc gatgagcacc atgtcctggt cggcgatgaa cgcgtcctgc tgctcggccc
     9121 agtggtcgta gtcggcgcgc ggcatggtcg gcttgccggt gggctgctcg gagacgaagt
     9181 aggtcgacgg ggcgagccgg gccgtcacca tggtcgtgta gctgatgttg ccgtgctcgc
     9241 tgacctgcac gcgcgacatg ttggcgagga cccagtcgcg cagctgctcc tgggacgggt
     9301 tgcgggtgac cgaggcggcg gtggggaggg tcatgcctca gtcccctacc cacgggacct
     9361 cagtcgatgc gacgcagctc ccgccggtac cggtgaccgc gctcgacgta gaactccgcc
     9421 gagtgcttga tcatctcgag gtccaccgag tcgtggcgga gcttggccgg cacgccgagg
     9481 gccatcgccc cggtgggcac ctcgaagccg ccgttgcaga ccgcgttggc accgaccagc
     9541 ccgccggtgc ggacgaccgc cttgtgcagc accacgctgc cggagccgac gagggcatcg
     9601 tcttcgatga tgcagccctc gaggtggacg atgtggccga tgacgcagcc gtcgccgatc
     9661 tgggtcggcg cccactcggt ggtgtggatg accgacccgt cctggatgga ggtgcggtcg
     9721 ccgatggtga tgggtccgct gtcgccgcgc agcacggcgc cgggccagat cgtggactcg
     9781 gatccgatgg tgacgtcgcc gatcaccacc gcatcggggt ggacgaaggc gtcgtcggcg
     9841 atggtggggt tcaggtcgcc cagggcgtag acgggcatgc agatctccgt ggcatggggt
     9901 gcgggcggtc gcccgccatc ctcgcagcgg tcacgacgtc ggagcgagtg cggtggtccg
     9961 gcgcacgacc cggcgcatgg atccgccggg cagcgtgccc gtgaggcgcc cgatgacgta
    10021 gagcaccggg aagatctcga ggcggccggc cagcatcatc accatcagca gcccgcgagc
    10081 cgggcgggag aagtagaggt agttggaggc cggaccggcc tcgccgagtg ccgggcccat
    10141 gttgcccatg gctgatgtgg cggcgccggc cgcctcggtc aggccggcgc cgaggaatgc
    10201 gagcgcgagg cagccgaggc cgatgaacac gacgtagagc agcgcgtagc cgatgacgcg
    10261 ctgcacgatc cgctccggca tcgcctcccg gccgagccgc acgggcagca cggcgcgggg
    10321 gtggcggatg cgtcgcagct cccgcacggc gtggacgagc agcacccggg cccggaccag
    10381 cttcagcccg cccgacgtcg atccggccat cccgcccgac accatgaggg cgagcagcag
    10441 cagctgggtg ccgggcaccc actgcacgta gtccgccgtc ccgaagccgg tggacgtgag
    10501 cagcgaggcc acgttgaacg tcgcgtcccg cagcgacttc ccgaacccga gaccctgctg
    10561 ggtccacagc agggcggtga tcgcggcggt gaacaccacg aacacgccca ggtagaagcg
    10621 cagcaggggg gactcggcgt agacccgcgg ccggcccgtc acggcgcgcc agtggagggt
    10681 gaagctggcg gccgacacca gcatcaggac cacgagcgcc agctcgacgg cgaccgagtc
    10741 gaacgcggcg atcgaggagt cgtaggggga gaggccgccg gtggagacgg cggtgaacgc
    10801 gtgggtgacg gcatcgaagg gggacatgcc caccgccagc agcagggcga tggcggccac
    10861 ggtgagcccg gcgtacacct gccacaggcg ccgggccgtc tcggagatgc gcggggcgag
    10921 gcggtcggtg gtgggtccgg gtgcctcggc gctcagcatg tccatgccgc cgacgccgag
    10981 gagggggagg atggccacgg cgagcacgac gatgcccgtg ccgccgtacc actgggtgag
    11041 gttgcgccag aacaggacgc cccgcggtgc cgcctcgatc ggtgcgagca cggtggaccc
    11101 ggtgccggtg aagcccgaga cggactcgaa cagcgcgtcg tcgatcgtgt cgaacgtgcc
    11161 ggtgagcagg taggggatgg cgccgacgat cgaggccagg atccaggtgc tgccgaccgc
    11221 gacgaacgtg gccgacgcgg tcatcttctc gggcacctcg gtgccccgcc acagggcgaa
    11281 gccggtcacg gcggcgatgc cggcggccgc catgagggcg gccgcctcgt ccccgccggc
    11341 gcccagctcg acgacggcgg agatgacgat gccggcggcg acgaagagca tggcggcgcc
    11401 gcccacgtgc ccgctgaggc tgggccagcg ccgggagggc tcccggaagc ggagctcggt
    11461 gtctcggctc cttcccggca tcccggcgac ggtagcgagc ggtcaccgaa ccgactgcac
    11521 ttctcccgcg gcgaccaata gcgtcgcccg ccgtgtcacg aatgcgttcc cccctcgccc
    11581 tcctggtcgc gctcgccctc ctcgccgccg catgcggtgg tgacgacgac gccacggccg
    11641 ccgacagcga gggcgacggc gacggcgaca cgggcggctt ggtcgtggtc acgacggtgg
    11701 cacccatcac ctcgatcgcc gccgacgtca tcggcgacct ggcccagatc cgcggtgtcg
    11761 tgcccgaggg caccaactcc cacacctacg agcccacgcc ctcggtgtcg gaggtgctgg
    11821 agggagccga cgtcgtgttc gccaacggcc tccagctcga ggagcccacg ctggcgctgg
    11881 cccgggacgt ggccggcgac gcgaccatcg tcgagctggg cgacctcatc gtcagccccg
    11941 acgagtacct ctacgacttc tcgttcccgg aggacggcgg caagcccaac ccccacctgt
    12001 ggaccgatcc gatgctcgcc aagggctacg cccgctacat cgccgagacc atgagcgacg
    12061 tcgacccgga caacgcggag gcctacgagg cgaaccgggc cgagttcgac gagatcgtgg
    12121 acgagctcga cgccgccttg cgcaccgccc tcgacaccgt cccggaagac aaccgcaagc
    12181 tggtgacgta ccacgacgcc tacgcgtact gggcccagac ctacggctgg acggtggtcg
    12241 gcgcggtgca acccgaggac ttcggcgacc cgtccccgcg cgccgtggcc gacctcatcg
    12301 accagatccg ggccgaggac gtccccgccg tgttcggctc cgaggtcttc ccgtccccgg
    12361 tgctggagac gatcgccgag gagaccggcg ccgactacgt cgccgacctg cgagacgacg
    12421 acctccccgg ccagccgggc gacgacgacc acagctggat ggccctgatg cgctccaact
    12481 acgcgacgat cgtcgaggtc ctcggcggcg acccggaggc gctcgagcag ctcgagctgc
    12541 ggcgcgtcgg ccccgatgcc gcggagtacc cgcagtgagc cagcggtagc gtcgctccgg
    12601 tgaccccgct cctcacgctc gagggagtcg ccgccgggta cggcgacggg tccgtcatca
    12661 ccggcgtcga cctgcagatc gaccgcggtg acttcgtcgg catcgtcgga ccgtccggtt
    12721 cgggcaagtc cacgctgctg cgcgtgctgc tcggcagcgt ggccgtgagc gagggccggc
    12781 tcgaccgaac gggcgggacg cgcctcggct acgtccccca ggtcgagcag atcgactggg
    12841 acttcccggt gaccgtggcc cagtgcgtgc tgatgtcgcg gacccgggga cggatcctcc
    12901 cgtgggcgtc gaaggcggag aaggccgacg tggcccgggt gctcgaccgg ctcggcatcg
    12961 gcgacctcgc cggccgccac atccgcgccc tgtccggtgg ccagcagcag cgggtgttcc
    13021 tcgcccgcgc catgctcgcc gaacccgacc tcctgctgct cgacgagccc acctccgggg
    13081 tcgacgtgcg cacccgccac gagatcctcc acctgctcgg cgagctccac gacggcggcc
    13141 tcggcatcgt cctcaccacc cacgacctca acggcatcgc cgcccacctg ccccggctgg
    13201 tctgcctgaa caagcaggtc atcggcgccg gcgcccccca cgaggtcctc gtcccgtcgg
    13261 tgctcgaggc cacgtacggc tcgcccatgg aggtgctcga gcacggcggc atgcgggtcg
    13321 tcgtcgacgt gccgagcgag gcccaggtca tccagctccg ggaccgcagc gcgtgatcac
    13381 cctcgacgag ctgctccggc cgttcgagtt ccagttcttc cagaacgggc tggtggtggc
    13441 caccatcgcc ggcgccctgt gcgggctcat cggcgtgtac gtcgtgctcc gctcgatgag
    13501 ctacatcggc cacgggctga gccacgccat cttcggcggc ttcgccgtct ccgccctcgc
    13561 gggcgtcaac gtgttcctcg gcgccggcct gtggggtctg gcgtcggcgc tcgccgtgtc
    13621 gtcggtgacc cgccggcgac cgatcggctc cgacgccgcc atcggggtca tcaccacggc
    13681 gtcgttcgcc ttcgggctcg ccgtgctgca gctggggggt ggcgccgacg tcgacgccga
    13741 cgccgccctc ttcggcaacg tcctcggcgt cacccggggt gacgtctgga tcgtgagcgg
    13801 ggtgaccctg ttcgccatcg ccgtcatcgt cctgcggtac cgctccctgc tcttcgtcac
    13861 cttcgacccc gaggtggccg aggcgtccgg cgtgcgggtg gcgcgcatcg atgcgctcct
    13921 gatgctcgtg ctcgccgcat cgatcctggc caccatgaac gtcatgggcg tcaccctcat
    13981 cgcggccgcc ctcgtcgtgc cggcagtggt cgcccggatg gtcacgtcct cgttctccac
    14041 gatgctgtgg ctgtcgaccc tcatcggggc ggtcagcggc ttcgtcggga tgaacctcag
    14101 ctaccacctc gacgtcgcat ccggccccac catcgtcctc gtgggcggtt cgttcttctt
    14161 gtgcgctttg gcggtcaccg gggtgcgcgg aaggacccgg ctggctgggg tgcaggccca
    14221 ctgagcacag gtagcgtccg ctccatggac tcccagcgca cgaagcgcat gctcgagtcg
    14281 gtgttgggcg tgtcggtcac cggcggcaac cgcatcgacg tgctgaagaa cggcgatcag
    14341 atcttcccgg ccatgctcga ggccatcgac gcggccgagc ggtcgatcga cttcctcacc
    14401 ttcatctact ggaacggcga gatcggccag gagttcgccg accacctgtc ggccaaggcc
    14461 cgggagggcg tgcgcgtgcg ggtcctgctc gacgccgccg gcgcctggga gctggacacc
    14521 gaggtggtgg acggcatgga tgaggccggc tgcgacgtgc gctggttccg gcccatcaag
    14581 gctcgcggcg cgctgtccgc caacaaccgc acgcaccgca agctcctggt gtgcgacgag
    14641 gagctcgggt tcacgggtgg tgtcggcatc gccgacgtgt ggggcggcga cgcccgaggc
    14701 gagggggagt ggcgcgacac ccacttccgg gtcgtcggcc ccgccgtggc cggcctgcgc
    14761 ggcgccttct tcgacaactg ggccgagacc ggcacggacc tggtggacga cgtcgaccag
    14821 ttcccggagc tctcccagcc cggcgagctc gacgtgcagg tcatcaaggg cgtcgccgag
    14881 gccggcaaca gcgacgtgtg gaacctgttc cgggtgctga tcgactgcgc cgaggagcgc
    14941 atccgcatca ccaccgccta cttcacgccg gacgaggaga tcctccgtcg cctgctgagc
    15001 gcgtcggagc gcggggtgga ggtcgaggtc ctcgtgcccg gtccccatgc ggacaagcgc
    15061 ctggtgcagg tggccagcga ggccagcttc cgcgagctgc tcgacgccgg cgtgcagatc
    15121 tgcacgttcc agccgtcgat gctccatgcc aaggtgctca cggtcgaccg cgcggtctgc
    15181 gtggtcggct cggcgaacat gaaccagcgc tcgaccgagc acgacgacga ggtctgcctg
    15241 gtcatcttcg acgaggccat gaccgcggtg ctcgacgggc acatggacga ggacctgcag
    15301 cgcgccgagc ccatcgacct cgacgagtgg gagaagcgag gcctcctcca gcggatcgag
    15361 gagaaggccg tgaaggtgat cgacgggctg ct
//
LOCUS       DATISU010000149         3854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_519530, whole genome shotgun sequence.
ACCESSION   DATISU010000149 DATISU010000000
VERSION     DATISU010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519530"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /gene="purM"
                     /locus_tag="VLR27_06165"
     CDS             complement(<1..983)
                     /gene="purM"
                     /locus_tag="VLR27_06165"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HSP03065.1"
                     /translation="MSEQQENTYASAGVDIGAGEEAVQRIKASVRSTFRPEVIGDIGG
                     FGGLFQFPQHRFTHPVLVSSTDGVGTKAVVAQRAGIFESIGVDLVAMCVDDIACAGAE
                     PLFFLDYIAVGKLDPDHIEQLVAGVAKGCREAGCALIGGEMAEHPGSMEAGEFDLVGF
                     AVGVAERDRILDGTTITEGDALIGLPSPNLRSNGYSLARRVFFDVAERGLHDPAWDGA
                     HHSLAEELLSPSVIYSPAIAALLRVVDVRAVAHITGGGIPGNLNRVLTESLDAHVDRG
                     AWEPPKVFTEIQRLGQVSDDEMAKVFNMGIGMIAVVPAEEVYAALDVLRTAG"
     gene            complement(983..2545)
                     /gene="purF"
                     /locus_tag="VLR27_06170"
     CDS             complement(983..2545)
                     /gene="purF"
                     /locus_tag="VLR27_06170"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HSP03066.1"
                     /translation="MTDPSDHAPQRDADPGAGDDELKEACGVFGIYAPGQSVAHLTYL
                     GMFALQHRGQEAAGMSVSDGTEIVTVKDNGLVSSVFTDRVLNGLPGHLAIGHDRYSTT
                     GASTWQNAQPVTREAAGHQISLGHNGNLVNTAELAEDLGMLPGSGAVTSDSDLVVEML
                     ARSIERSERHRSDDRHLEAAMLEVMPKLKGAFSIVCMDEGHVVGVRDPNGFRPLCLGR
                     LESGWVLASETPALDLVGASFVREIEPGEMIVIDATGYRSVRAFPESEVNPSLCLFEF
                     VYFARPDSTLYGKNVHSARVRMGEQLAQQAPVEADMVMGVPESGLPAAEGFARQSGIP
                     VGAGLVKNRYIGRSFIAPSQEARAAAVRMKLNPLKENLQGKRLIVVDDSIVRGTTQRT
                     LTQMLREAGAVEVHLRITSPPVMWSSFYGIDTGVRSELLASNLDIEEIRAYLGVDSLA
                     YLGLDRLITATGTPSSGFCTACFTGDYPVEIPVSLSKAVLESPALRTEAPSGIATALA
                     SRVGGSQMGFDD"
     gene            complement(2622..3641)
                     /locus_tag="VLR27_06175"
     CDS             complement(2622..3641)
                     /locus_tag="VLR27_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03067.1"
                     /translation="MSDDQDHPRPGGDARTERTLDELLALRADVAALLGRFASLQRSL
                     VGEGQHSLIEQLQDNLADVASGDVVSALWDEVRAVRAQLDEAATSVAPVVAAPVEPAG
                     PDPQLAALTDELAVLRTELADGLVVEPSDALSASLDALRSEVDGLREALGELRDAPVP
                     APADGGRDDDAVLQRLGTIEAGIDELRGRLDEGLVLADDTELPAPAAGGGADVEAIAD
                     QVAALRDFVASELDTVRQTVSARIDAATETTVAAASARPEPAPAPAVGSATIDPDTLE
                     QLREEIRAAGTVSDQVIDALRDELKALRRRIAVKATERVLDDQQLAQIADAVAARLAD
                     DRDRA"
BASE COUNT          523 a         1306 c         1478 g          547 t
ORIGIN      
        1 ccggcggtgc gcagcacgtc gagggcggcg tagacctctt cggcggggac gacggcgatc
       61 atgccgatgc ccatgttgaa caccttggcc atctcgtcgt cgctgacctg gccgaggcgc
      121 tggatctcgg tgaagacctt cggcggctcc caggcacccc ggtcgacgtg ggcgtcgagg
      181 gactcggtga gcacgcggtt caggttgccg gggatgccgc ccccggtgat gtgggcgacg
      241 gctcgcacgt cgacgacccg gagcagggcg gcgatggccg gcgagtagat gaccgacggt
      301 gacagcagct cctcggccag ggagtggtgg gcgccgtccc aggcgggatc gtgcaagccg
      361 cgctcggcga cgtcgaagaa cacccgacgg gccagcgagt agccgttgga gcgcaggttc
      421 ggcgacggga gcccgatgag ggcatcgccc tcggtgatgg tggtgccgtc gaggatgcgg
      481 tcgcgctcgg cgaccccgac ggcgaagccg acaagatcga actcgccggc ctccatcgaa
      541 ccggggtgct cggccatctc gccgccgatg agcgcgcagc ccgcctcccg gcagcccttg
      601 gcgacgcccg cgacgagctg ctcgatgtgg tcggggtcga gcttgcccac cgcgatgtag
      661 tcgaggaaga acagcggctc ggccccggcg caggcgatgt cgtcgacgca catcgcgacg
      721 aggtccacgc cgatcgactc gaagatgccg gcccgctggg ccaccacggc cttggtgccg
      781 acgccgtcgg tggaggacac cagcaccggg tgggtgaagc ggtgctgggg gaactggaac
      841 agcccgccga agccgccgat gtcgccgatg acctcggggc ggaacgtgga gcggaccgac
      901 gccttgatgc gctggaccgc ctcctcgccg gcaccgatgt cgacgccggc ggacgcgtag
      961 gtgttctcct gctgctccga catcagtcgt cgaaccccat ctgggagccg ccgacccggg
     1021 aggccagcgc ggtggcgatg ccgctcgggg cctcggtccg cagggcgggg ctctcgagca
     1081 cggccttgga cagcgagacc gggatctcca ccggatagtc gccggtgaag caggccgtgc
     1141 agaacccgct ggagggggtg ccggtggcgg tgatcaggcg gtcgagcccc aggtaggcga
     1201 gcgagtcgac ccccaggtag gcccggatct cctcgatgtc gaggttggag gcgagcagct
     1261 cactgcgcac gcccgtgtcg atgccgtaga agctggacca catgaccggg ggcgaggtga
     1321 tgcgcaggtg cacctcgacg gcaccggcct cgcgcagcat ctgggtcagc gtgcgctggg
     1381 tggtgccgcg gacgatggag tcgtcgacga cgatcaggcg cttgccctgg aggttctcct
     1441 tcagggggtt cagcttcatc cggaccgcgg cggcccgggc ctcctgggag ggggcgatga
     1501 agctgcgccc gatgtagcgg ttcttcacca ggccggcgcc gacggggatg ccggactggc
     1561 gggcgaagcc ctcggcggcg ggcaggccgg actccggaac gcccatgacc atgtcggcct
     1621 cgacgggtgc ctgctgggcc agctgctcgc ccatgcggac ccgggccgag tgcacgttct
     1681 tgccgtagag cgtggagtcg gggcgggcga agtacacgaa ctcgaacagg cacaggctcg
     1741 ggttgacctc ggactcgggg aaggcccgga ccgagcggta gccggtggcg tcgatgacga
     1801 tcatctcgcc cggttcgatc tcgcgcacga agctggcgcc gaccaggtcg agcgccgggg
     1861 tctccgatgc gagcacccag ccggactcga gccggcccag gcacagcggg cggaagccgt
     1921 tgggatcgcg cacgccgacc acgtggccct cgtccatgca gacgatggag aacgcgccct
     1981 tgagcttggg catgacctcg agcatcgccg cctcgaggtg gcggtcgtcg ctgcggtggc
     2041 gctcgctgcg ctcgatggac cgggcgagca tctcgacgac gaggtcgctg tcagaggtga
     2101 ccgcacccga cccggggagc atgcccaggt cctcggccag ctcggcggtg ttgaccaggt
     2161 tgccgttgtg cccgagggag atctgatggc cggcggcctc acgggtgacc ggctgggcgt
     2221 tctgccacgt gctggcaccg gtggtgctgt agcggtcgtg gccgatggcc aggtggccgg
     2281 ggaggccgtt gagcacccgg tcggtgaaca ccgacgagac caggccgttg tccttgaccg
     2341 tgacgatctc ggtgccgtcc gacaccgaca tgccggcggc ctcctggccc cggtgctgga
     2401 gggcgaacat gcccaggtac gtcaggtggg cgacggactg gccgggcgcg tagatgccga
     2461 agaccccgca ggcctccttc agctcgtcgt cgcctgcgcc ggggtcggcg tcgcgttggg
     2521 gggcatgatc ggacggatcg gtcatgggtc ggcgacacct ccggcgtcgg gtccgaggct
     2581 accggtcgac cggccccgat cgaccaaccc agcggcggat gtcaggcccg gtcccggtcg
     2641 tcagcgagcc gggcggccac ggcgtcggcg atctgggcca gctgctggtc gtcgaggacc
     2701 cgctcggtcg ccttcaccgc gatccggcgg cgcagcgcct tgagctcgtc gcgcagggca
     2761 tcgatgacct ggtccgagac ggtgccggcg gcgcggatct cctcgcgcag ctgctccagc
     2821 gtgtcggggt cgatggtggc gctgcccacc gccggtgccg gcgcgggctc gggtcgggcc
     2881 gaggcggccg cgacggtggt ctcggtggcg gcgtcgatgc gggccgagac ggtctggcgc
     2941 acggtgtcga gctcggaggc gacgaagtcc cgcagcgcag ccacctggtc ggcgatggcc
     3001 tccacatcgg ctcccccgcc ggcggcgggg gcaggcagct cggtgtcgtc ggccaggacc
     3061 aggccctcgt ccaggcggcc gcgcagctcg tcgatgccgg cctcgatggt gccgagccgc
     3121 tggagcacgg cgtcgtcgtc gcggccgccg tcggccggtg ccgggaccgg tgcatcccgc
     3181 agctcgccga gcgcctcgcg caacccgtcg acctcggacc ggagggcgtc gagcgaagcc
     3241 gacagcgcgt cggagggctc gaccacgagt ccgtcggcca gctcggtgcg caggacggcg
     3301 agctcgtcgg tcagggccgc cagctggggg tccggtcccg ccggttcgac gggtgccgcg
     3361 acgacggggg cgacgctggt cgcggcctcg tccagctggg cccgcacggc ccgcacctcg
     3421 tcccagaggg cgctgacgac atcgcccgac gcgacgtcgg ccaggttgtc ctgcagctgc
     3481 tcgatcaggc tgtgctggcc ttcgccgacg aggctgcgct gcagcgaggc gaaccggccg
     3541 aggagggcgg cgacgtcggc ccgcagggcg agtagttcgt cgagggtccg ctcggttcgg
     3601 gcatcgccgc cggggcgcgg gtggtcctgg tcgtcgctca tggtgcctcc gtgtcgggct
     3661 gcgtgcgcca gccatcctgc cccatcaccg gcctgaggtc ggccattcgc aggcgcgctg
     3721 gataccctcc ccgggatgac gtccctcgcc gacgccgggc ctgacaccgt gctcgggcgc
     3781 cgcccgtgga tcctgaagct cgccaagtac tcggccgcgt cgctcgccgg ggtcgtcacc
     3841 agcacgagca cgct
//
LOCUS       DATISU010000150         4142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_752029, whole genome shotgun sequence.
ACCESSION   DATISU010000150 DATISU010000000
VERSION     DATISU010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752029"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VLR27_06180"
     CDS             complement(<1..204)
                     /locus_tag="VLR27_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HSP03068.1"
                     /translation="MATAVIVDAVRTPGGKRFGQLSGWHPSDLAAEVLKALAERNDLD
                     PALVDDVVMGCVMQVGNQGLNIGR"
     gene            323..1636
                     /locus_tag="VLR27_06185"
     CDS             323..1636
                     /locus_tag="VLR27_06185"
                     /inference="COORDINATES: protein motif:HMM:NF019626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavohemoglobin expression-modulating QEGLA
                     motif protein"
                     /protein_id="HSP03069.1"
                     /translation="MAETYEETVRALSDAIVDAQRPVRILDAIKWDDQVRDAFFASGC
                     REQPAVDAAWYEQRDLGFDLDAAREQFLDIELWINTSLGTTSTLSQLLKRTCEQYRLT
                     LDMLEARGTDGFGPLAAQLYGTTDDVFHAAGPRVADLAGQFRDALSWVSDLPIDERDE
                     KGIDGGSAVALLQARLDASMGPGMVHVVLDDGIVADAAAGSDYIKLRADAMFSERQLE
                     QLESHEGWVHVGTSDNGRAQPYCTFLGKAPPPVNVTQEGLATLTEILSLTSHPIRLLR
                     IADRIEAIALAEDGASFVEVVEHFRTLGLDEDECWQLVMRVFRGSTPTGRPFTKDLGY
                     GKGLVLTYAYVRVAIRTGRLDRVPLLFCGKVDINQMGAVNALYDEGLVAPPAFLPEVF
                     RDVNALAAQVAFSRFLRDLDFDRLAADYAPLLGAPAVIDEPDALL"
     gene            complement(1652..1996)
                     /locus_tag="VLR27_06190"
     CDS             complement(1652..1996)
                     /locus_tag="VLR27_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03070.1"
                     /translation="MGRACSEADPTTQHRDRTEIGAEADAAADAGGSFVGRRPGLVSL
                     LAGGVGVVVATLPGLGLLALALAALALATGVPAMRRGPRSRCFSTARTGVVLGMIAVL
                     LGFVSLAMQLLG"
     gene            2041..3645
                     /locus_tag="VLR27_06195"
     CDS             2041..3645
                     /locus_tag="VLR27_06195"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HSP03071.1"
                     /translation="MTGSSPSPWSRRRFLGVAGAGAASLALGACQDDGNAERGPVDPS
                     ATTSTAPPEPELETLEAIDHGAPQGPPPFGLGVASGDPDHESVVLWTWVDREPSAPVP
                     LLVEVARDDAFESIVAREELVADPAEAMTARIVVAGLAPDTRHWFRFVLDGHLTPAAR
                     TRTAPAPGDLPDQPLRVGHLSCQRWSSGYWTALDDVADQAPDLVVHCGDYVYETDGGS
                     VRPIDEPAPTDLDGYRALWRRYKAEPELQAAHAVAPWLITWDDHEVVNNYQGTATVDG
                     PGGEEFLARRAAAYRAWWEFTPTRLDPPDGPSLDIHRVVDWGGLTRFVLLDTRQFRDE
                     QPCDDSLGTDLGPRCDETAITTMLGDAQEAWFDDVATGHDAVWTTVVQQVVVHQWRIA
                     PGNVAWNLDQWDGYTGARRRFLETLSRSAGPVVLSGDVHSSWVADLPVDFEEDEAPIV
                     GAEFVAPGVSSDIPDRLRQASGLVEALSPHIAWSETTQRGWVLHEVDADAWRAAYRLV
                     DDVTVPDGTVTEATTWAIDAGTPGLA"
     gene            3688..>4142
                     /locus_tag="VLR27_06200"
     CDS             3688..>4142
                     /locus_tag="VLR27_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03072.1"
                     /translation="MTGTIRGAAHLAGAYEHPLRTIPDRTTPQIHAEVAVGALADAGL
                     SMGDVDAYFCAGDAPGFGPLSMAEYLGLDRLSYVDSTEIGGSSYVAHVGHAAAAIAAG
                     KCRVALITLAGKPRTGGSGPGGSGRVGDAPEFSFEVPFGTSVLSNYALAA"
BASE COUNT          563 a         1560 c         1457 g          562 t
ORIGIN      
        1 gcggccgatg ttgaggccct ggttgccgac ctgcatgacg cagcccatga cgacgtcgtc
       61 gacgagcgcg gggtcgaggt cgttgcgctc ggccagggcc ttgagcacct cggcggccag
      121 gtcggacggg tgccagccgg acagctggcc gaagcgcttg ccaccggggg tgcggacggc
      181 gtcgacgatg acggcggttg ccatgggtgt ctcctacggg atcgcgaaag ttgaccgatc
      241 agtcaagacg agtctgtctg gctcgtccgg tttcccgcaa gaagcgcagg gacccggcgc
      301 catcggccat ccttgggggg acatggcaga gacctacgag gagacggtgc gcgccctttc
      361 ggacgcgatc gtcgacgccc agcgcccggt gcggatcctc gacgccatca agtgggacga
      421 ccaggtgcgc gacgcgttct tcgcctccgg gtgccgcgag cagccggcag tggacgccgc
      481 ctggtacgag cagcgggacc tcggcttcga cctcgacgcg gcccgcgagc agttcctcga
      541 catcgagctg tggatcaaca cctcgctcgg caccacgtcc acgctctccc agctgctcaa
      601 gcggacgtgt gagcagtacc gcctcaccct cgacatgctc gaggcgcgcg gcaccgacgg
      661 gttcggtcct ctcgccgccc agctgtacgg caccaccgac gacgtcttcc acgccgccgg
      721 accccgggtc gccgacctcg ccggccagtt ccgcgacgcc ctcagctggg tgtccgacct
      781 gcccatcgac gagcgcgacg agaagggcat cgacggcggg tcggcggtcg cgctgctgca
      841 agcccgcctc gacgcctcga tgggtccggg catggtgcac gtcgtgctcg acgacggcat
      901 cgtcgccgac gccgccgccg ggtccgacta catcaagctg cgcgccgacg ccatgttctc
      961 ggagcgccag ctcgagcagc tcgagtccca cgagggctgg gtgcacgtcg gcaccagcga
     1021 caacgggcgg gcccagccgt actgcacctt cctcggcaag gcgccgccgc cggtcaacgt
     1081 gacccaggag ggactggcca ccctcaccga gatcctgtcg ctcacctcgc accccattcg
     1141 cctgctgcgc atcgccgacc gcatcgaggc catcgccctc gccgaggacg gcgccagctt
     1201 cgtggaggtc gtcgagcact tccgcacgct cgggctggac gaggacgagt gctggcagct
     1261 ggtcatgcgg gtcttccggg gctccacccc cacgggtcgg cccttcacca aggacctcgg
     1321 ctacggcaag gggctcgtcc tcacctacgc ctacgtccgg gtcgccatcc gcaccggccg
     1381 cctcgaccgg gtgccgctgc tgttctgcgg caaggtcgac atcaaccaga tgggcgcggt
     1441 gaacgccctc tacgacgagg gtctcgtcgc gccgcccgcg ttcctgcccg aggtgttccg
     1501 cgacgtgaac gccctggcgg cccaggtcgc gttcagccgg ttcctgcgcg acctcgactt
     1561 cgaccgcctc gccgccgact acgcccccct gctcggcgca ccggcggtca tcgacgaacc
     1621 cgacgcgctg ctctgaaccc ggcgggtgcg atcagcccag cagctgcatc gccaggctga
     1681 cgaagccgag gagcacggcg atcatgccga gcaccacgcc ggtgcgggcg gtcgagaagc
     1741 accgggaccg ggggccgcgt cgcatcgccg ggacgcccgt ggcgagggcg agggcggcca
     1801 gtgcgagggc gagcagcccg aggccgggca gggtggcgac caccacgccg acgccgccgg
     1861 ccagcaggga caccaggccg ggtcggcgac cgacgaagct cccgcccgcg tcagcggcgg
     1921 cgtcggcttc cgcaccgatc tcggtgcggt cgcggtgctg cgtcgtcggg tcggcctcgg
     1981 agcacgctct acccaacccg gccgggtacc accgcgcgat ccggttacgg tcgaccggcc
     2041 atgactggat cgtccccgtc gccgtggtcg cgacgacggt tcctcggggt ggcgggggcc
     2101 ggcgccgcct ccctcgcgct gggcgcgtgc caggacgacg gcaacgccga gcgcggtccg
     2161 gtcgacccct cggcgaccac ctcgaccgcg ccgcccgaac ccgagctgga gaccctggag
     2221 gccatcgacc acggtgcgcc gcaggggccg ccgccgttcg ggctcggcgt ggcgtcgggt
     2281 gacccggacc acgagtcggt cgtgctgtgg acgtgggtcg accgggagcc gtcggcgccg
     2341 gtgccgctgc tggtggaggt cgcccgggac gacgcgttcg agtcgatcgt cgcccgcgag
     2401 gagctcgtcg ccgacccggc cgaggcgatg accgcccgca tcgtcgtggc cggcctggcg
     2461 cccgacaccc gccactggtt ccgcttcgtg ctcgacggcc acctcacacc ggcggcccgg
     2521 acccggaccg cgccggcgcc gggagacctg ccggaccagc ccctgcgggt cgggcacctg
     2581 tcgtgccagc gctggtcgtc gggctactgg accgcgctcg acgacgtggc cgaccaggcg
     2641 cccgacctcg tcgtgcactg cggcgactac gtctacgaga ccgacggcgg atcggtgcgg
     2701 cccatcgacg agccggcgcc caccgacctc gacggctacc gggccctgtg gcggcggtac
     2761 aaggccgagc ccgagctgca ggccgcccac gccgtcgccc cctggctcat cacctgggac
     2821 gaccacgagg tggtgaacaa ctaccagggc accgccacgg tggacggacc gggtggtgag
     2881 gagttcctcg ctcgtcgtgc tgccgcgtac cgggcgtggt gggagttcac ccccacccgg
     2941 ctcgaccccc cggacggacc gagcctcgac atccaccgcg tcgtggactg gggcggcctg
     3001 acccgcttcg tgctgctcga cacccgccag ttccgcgacg agcaaccctg cgacgactcc
     3061 ctcggcaccg acctcggccc ccgctgcgac gagaccgcga tcaccaccat gctcggcgac
     3121 gcgcaggagg cgtggttcga cgacgtcgcc accgggcacg acgcggtctg gaccaccgtc
     3181 gtccagcagg tcgtcgtcca ccagtggcgc atcgcccccg gcaacgtggc gtggaacctg
     3241 gaccagtggg acggctacac gggcgctcgc cgccggttcc tcgagacgct ctcgcgaagc
     3301 gccggtccgg tcgtgctcag cggcgacgtc cactcgtcgt gggtggccga cctgccggtg
     3361 gacttcgagg aggacgaggc gccgatcgtc ggtgccgagt tcgtcgcgcc cggcgtgtcc
     3421 tcggacatcc ccgaccgcct ccgtcaggcg tcgggactcg tcgaggccct gtccccccac
     3481 atcgcctggt ccgagaccac ccagcggggt tgggtgctcc acgaggtgga tgccgacgcc
     3541 tggcgagccg cgtaccgcct ggtcgacgac gtgacggtgc cggacgggac ggtcaccgag
     3601 gcgacgacct gggccatcga cgccgggacg ccggggctgg cctgacctct ttggcccggg
     3661 gcggtggcgc tgggcagact ccccgccgtg acggggacga tcaggggagc agcacatctg
     3721 gcgggcgcgt acgagcaccc cctgcggacg atcccggacc ggacgacgcc gcagatccac
     3781 gccgaggtcg ccgtgggcgc gctcgcggat gccggactgt cgatgggcga cgtcgacgcc
     3841 tacttctgcg ccggtgacgc cccgggcttc gggccgctct cgatggcgga gtacctcggc
     3901 ctcgatcgcc tgtcgtacgt ggacagcacc gagatcggcg gctcgtccta cgtggcccac
     3961 gtcggccacg ccgccgctgc catcgccgcc ggcaagtgcc gggtcgcgct gatcaccctg
     4021 gccggcaagc cccgcacggg cggctccggc cccggcggca gcgggcgcgt cggcgacgcc
     4081 ccggagttct ccttcgaggt ccccttcggc acgtcggtgc tgtcgaacta cgccctcgcc
     4141 gc
//
LOCUS       DATISU010000151         6612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_239820, whole genome shotgun sequence.
ACCESSION   DATISU010000151 DATISU010000000
VERSION     DATISU010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239820"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VLR27_06205"
     CDS             <1..399
                     /locus_tag="VLR27_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HSP03073.1"
                     /translation="EEYHRGHSNIDSVVEGLGATAKVITAAALIMISVFLGFVASDDP
                     VVKMMGVGLATAVAVDATIVRMVLVPSTMALVGDANWWLPRWLDRILPSLDLEADPEP
                     ATFAIEFHRIPSAPAPEAGGESTGLRVREL"
     gene            complement(386..763)
                     /locus_tag="VLR27_06210"
     CDS             complement(386..763)
                     /locus_tag="VLR27_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSP03074.1"
                     /translation="MVDDAEQIADALFGAIEGGDLDAVAALYSPDVEVWLNTDGIARG
                     RDENLRTLGWVVANLTDRRYEEIRRHRTDTGVVQQHVLRATTAAGRRVAVPACMVITI
                     DGGRITRLEEYLDSAHIAELTAP"
     gene            complement(756..1865)
                     /locus_tag="VLR27_06215"
     CDS             complement(756..1865)
                     /locus_tag="VLR27_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03075.1"
                     /translation="MEQVTREWVALPSPTAGRAGHGSHPCQGIYHRAADASPRCAFVA
                     THYNVDFSEHYLAEHLARRGFGFLGWNTRYRGNEAHFLLEHALVDIGVGVRWLRDVAG
                     VETVVILGNSGGGSLMAAYQSQATAPVIAPARDLSLPDEVLDLPSADLYVSLNAHPGR
                     PDVLTAWLDPSVTDEADPLAVDASLDMFDDRNGPPYAPGFVARYRSAQEERNHRITDW
                     AIAELERLGAAGAWDRLFDVHRVWADLRFTDLALDPSDRRPGCYAGDPKRANLGPFGI
                     GRTNTLRSWLSMWSLRESQCVGAPHLAQIEVPSLVVQSTADRGVFPSDARAIHDALAA
                     EDKTLRFLPGEHYFEDGDRAEVADLIAAWVEDRRG"
     gene            2062..2646
                     /locus_tag="VLR27_06220"
     CDS             2062..2646
                     /locus_tag="VLR27_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03076.1"
                     /translation="MTVIRVNPESVRAYGSDAQARFEAIRAELIGLVTAVTEVRYFGP
                     NAVDFKTRSGQMAADFANKLTQDMGAIAEAIRTSTTNIASSLGGAPVVINVAGGTISP
                     PAVQSVDYVDVDTSALEALKGVVDRHFSMIIDSFEQHLQRLMSTDWQGNAREQAVSQV
                     QGFTATAKSRAAEAQASLNQYISSQLQSVTTADR"
     gene            complement(2654..>6612)
                     /locus_tag="VLR27_06225"
     CDS             complement(2654..>6612)
                     /locus_tag="VLR27_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HSP03077.1"
                     /translation="PHEDPQNGVLVNDEPIDEATVVGPDDVVALGSSRVSFREFIRAE
                     HEAIDRLGQIEFHRTPYRPDKVRERELEPFGPIPARPEPRRFQLLAVLAPLAAGALLF
                     AFSRQVQFLAIIAISPLVMVGTWIEDRRSGRHRFRDDVERFEERLLAFESEIAAAREA
                     ERVERVRAAPDLADLARRAELRTIDLWPRGRHDEDFLRVRLGLGDAPSLVAATLASGG
                     DDDYRERVEAVIAGHDTIGDVPVTVGLADLGVVGVHGPPEEVDDVVSSVLVQVACLHS
                     PEDLVVVGAVGADRPVAEWLKWLPHVRSATSPLAGSHLCVDADDARRLLNGLVEVADR
                     RLAEADNRVDRRWPWLLAVLDGDLGLDATVVSQLLDRAQAAGISVLWCSSSEGNVPRQ
                     ARSVVDCGPPQEPGFARLWSTDPDVDDQTVELGQVHPDVADRVARSLAAVRDASVATA
                     TTAIPRVVPLLSVLGTDRPDAEWVLRRWRTDTGYGLAHPVGLGPDGVFSLDLVADGPH
                     ALIGGTSGAGKSELLQSMVASLISCHPPNRLNLLFVDYKGGASSTVFRDVPHTVGYVT
                     NLDATLSRRALTSLRAELDRRMRLMEGRAKDLAEMLEHHPDDAPPSLVIVVDEFATLV
                     KEVPEFVAGVVDIAQRGRSLGIHLILATQRPSGSVNENILANTNLRIALRMLDNAESG
                     SVIGSPEAAGIPVPLRGRGYARLGPHDLVPFQSAFAGAPLDGDAQRGVVVVRSFDTTG
                     VGAPLASQSAADGGADASASGPTTHLEAVLDAVIAAHARTGLPASRPPWREMLPDHVT
                     LDALAADPRYEIAPEDAGRVIALGLLDAPEDQDQYPALVDLEDGGGLLVFGAGGAGKT
                     TLLRTAVVSATRAAAPGGLTVFGIDFASRALKNLEALPEVAAVAGGDDLEGVTRIIAL
                     LGAELERRRTLLADARADTLSAYRAQGGDDDLPRILLVVDGYANLVASMTGSSFSSNL
                     DEWLEGLHRVVIDGRQAGIHVVLAAERRAGVPALVQSAIAHRLVLRQADPAGYAELGI
                     PLSQAADLDLGPGRGLWRNGLLVQVACVSEDGDGRAQADAVLDEAARRPDRPAPIRTQ
                     PLPVAVPSVDLPAPRDGRVPIGLADLTLDPVWVDVDHSHVLITGVPRSGRSTAARVVV
                     DGLECLGHEVWKVGRAGSPLAEEADLHRSAFGRPAAIQPVLEELAELLDGSPTAIPRV
                     LAADDLDALEDASLNPLWERIAGHDAVRVVATIENRAFSGFSMNALLNEVRGARRVLV
                     LRPDDPLELFQMTGVKAQIRPGTPMPPGRGVLLTDRIPTLVQVAQPSAAPEVVGRR"
BASE COUNT          922 a         2325 c         2516 g          849 t
ORIGIN      
        1 gaggagtacc accggggcca cagcaacatc gacagcgtcg tcgagggcct cggtgccacc
       61 gccaaggtca tcaccgccgc cgcgctcatc atgatcagcg tcttcctcgg cttcgtcgcc
      121 agcgacgatc ccgtcgtcaa gatgatgggc gtcgggctcg ccaccgccgt cgccgtcgac
      181 gccaccatcg tgcgcatggt gctcgtcccg tccaccatgg ccctcgtcgg cgacgccaac
      241 tggtggctgc cacgttggct cgaccgcatc ctgcccagcc tcgacctcga agccgacccc
      301 gagcccgcca ccttcgccat cgagttccac cgcatcccca gcgccccggc ccccgaggcg
      361 ggaggcgaga gcacggggct ccgcgtcagg gagctgtgag ctcggcgatg tgggctgagt
      421 cgaggtactc ctcgaggcgc gtgatccttc cgccgtcgat ggtgatgacc atgcaggccg
      481 gcacggcgac ccggcgtccc gccgcggtcg tcgcccgcag gacgtgctgc tgcacgacac
      541 cggtgtcggt ccggtgccgg cggatctcct cgtagcgacg gtcggtcagg ttcgcgacca
      601 cccagccgag cgtccggagg ttttcgtccc ggccgcgcgc gatcccgtca gtgttgagcc
      661 agacctccac gtcggggctg tagagcgcgg cgaccgcatc gaggtcgccg ccctcgatgg
      721 caccgaacag ggcatcggcg atctgctcgg cgtcgtcaac cacggcggtc ctccacccag
      781 gcggcgatga ggtcggcgac ctcggcgcga tccccgtcct cgaagtagtg ctcgccgggc
      841 aggaaccgca gggtcttgtc ctcggcggcg agcgcgtcgt ggatggcgcg ggcatcggag
      901 gggaacacgc ctcggtccgc ggtggactgc accaccagcg acggcacctc gatctgggcc
      961 aggtgcggtg caccgacgca ctgggactcg cgcaggctcc acatcgacag ccaggatcgc
     1021 agcgtgttgg tccggccgat gccgaagggt ccgaggttcg cccgcttcgg gtcaccggcg
     1081 tagcagccgg gccgacggtc cgagggatcc agtgcgaggt cggtgaagcg gaggtcggcc
     1141 cacacccggt gcacgtcgaa gagccgatcc cacgccccgg ccgcgcccag ccgctccagc
     1201 tcggcgatgg cccagtcggt gatgcggtgg ttccgctcct cctgcgcgct gcggtacctg
     1261 gccacgaagc ccggggcgta cggcgggccg ttgcggtcgt cgaacatgtc gagggatgca
     1321 tcgacggcca gcgggtcggc ctcgtcggtc acggacgggt cgagccaggc ggtgagcacg
     1381 tcggggcggc cgggatgcgc gttgagcgac acgtacagat ctgcgctcgg caggtcgagg
     1441 acctcatcgg ggagcgacag gtccctcgcg ggcgcgatga cgggtgccgt ggcctgcgac
     1501 tggtacgccg ccatcagcga cccgcccccg gagttgccga ggatgacgac ggtctcgacg
     1561 ccggcgacgt cgcgcagcca gcggacgccg acgccgatgt cgaccagggc gtgctcgagc
     1621 aggaagtgcg cctcgttgcc ccggtagcgg gtgttccacc cgaggaaccc gaagccccgc
     1681 cgggccaggt gctctgccag gtagtgctcc gagaagtcca cgttgtagtg cgtggcgacg
     1741 aacgcgcacc gtgggctcgc atcggcggcg cggtggtaga tcccctgaca cgggtggctg
     1801 ccgtgaccgg cccgcccggc cgtgggggag gggagcgcca cccactcgcg cgtcacctgc
     1861 tccatgggtc cccctcccgc atccctcgga tgcccgtcgc ccgaacccta cgcaaccccc
     1921 cggaagcacc gaaaggtgac acgtacatgg acagatccgc gttgaggcag gctgggggcc
     1981 acccgatggc gaagactcgg gggacaacgg cggcggcggg acgaccctcc gcgacacacg
     2041 tgcaagacaa ggagagtcct catgaccgtg atccgcgtca accccgagag cgtgcgggcc
     2101 tacggcagcg atgcccaggc ccggttcgag gccatccggg ccgagctgat cggcttggtc
     2161 accgcggtga ccgaggtgcg ctacttcggc cccaacgccg tcgacttcaa gacgcggtcc
     2221 gggcagatgg cagccgactt cgccaacaag ctgacccagg acatgggcgc catcgccgag
     2281 gcgatccgca cgtccacgac caacatcgcc tcgtccctcg gtggcgcacc ggtggtcatc
     2341 aacgtggccg gcggcaccat cagcccgccg gcggtgcaga gcgtcgacta cgtcgatgtc
     2401 gacacctcgg cgctcgaggc cctcaagggc gtggtcgacc gccacttctc gatgatcatc
     2461 gacagcttcg agcagcacct ccagcggctg atgtcgaccg actggcaggg caacgcgcgc
     2521 gagcaggccg tctcccaggt gcaggggttc accgccaccg ccaagtcgcg cgccgcggag
     2581 gcccaggcgt cgctgaacca gtacatctcc tcgcagctgc agtcggtcac caccgccgac
     2641 cgctgagcgc cggtcatctc cgacccacga cctccggagc ggcggagggc tgagccacct
     2701 gcaccaacgt cgggatccga tcggtcagca gcacgccccg gccgggcggc atcggcgtcc
     2761 ccggccggat ctgggccttc acgccggtca tctggaacag ctcgagggga tcgtccgggc
     2821 gcagcaccag cacgcggcgg gcgccccgca cctcgttgag cagggcgttc atggagaagc
     2881 cactgaaggc ccggttctcg atggtggcca cgacccggac ggcgtcgtgg ccggcgatgc
     2941 gctcccagag cgggttgagc gaggcgtcct cgagggcgtc gaggtcgtcg gcggccagca
     3001 cccgggggat cgcagtcggc gagccgtcga gcagctcggc cagctcctcc agcaccggtt
     3061 ggatcgcggc cggtcgcccg aacgcgctgc ggtgcaggtc cgcttcctcg gccagcgggc
     3121 tgccggcgcg gcccaccttc cagacctcgt ggccgaggca ctccagcccg tcgacgacca
     3181 cgcgcgccgc ggtgctccgt ccggagcggg gcacgccggt gatcagcacg tgggagtggt
     3241 cgacgtcgac ccacaccggg tcgagggtga ggtcggcgag cccgatgggc acccgaccgt
     3301 cccggggggc cggcaggtcg acggacggga cggcgacggg gagcggctgg gtgcggatcg
     3361 gcgccggccg gtcggggcgt cgggcggcct cgtccagcac ggcgtcggcc tgggcccgcc
     3421 cgtcgccgtc ctcgctgacg caggcgacct ggacgagcag cccgttgcgc cacaggccgc
     3481 gacccggccc gaggtcgagg tcggcggcct ggctgagcgg gatgccgagc tcggcgtagc
     3541 cggcggggtc ggcctggcgc aggaccaggc ggtgggcgat ggccgactgc accagcgcgg
     3601 gcacgccggc tcggcgctcg gcggcgagga ccacgtggat gccggcctgg cgtccgtcga
     3661 tcacgacccg gtgcagaccc tcgagccact cgtcgaggtt cgacgagaag ctgctgccgg
     3721 tcatcgacgc cacgaggttg gcgtagccgt cgaccaccag caggatccgg ggtaggtcgt
     3781 cgtcgccgcc ctgggcccgg taggcgctga gcgtgtcggc ccgggcgtcg gccagcaggg
     3841 tgcggcggcg ttcgagctcg gcgccgagca gggcgatgat gcgcgtgacc ccctcgaggt
     3901 cgtcaccgcc ggcgacggcc gccacctcgg ggagcgcttc gaggttcttc agcgcccggg
     3961 acgcgaagtc gatgccgaac acggtgagcc cgccgggggc cgccgcgcgc gtcgccgaga
     4021 cgacggcggt gcgcagcagg gtggtcttgc cggcgccgcc ggcgccgaac accagcaggc
     4081 cgccgccgtc ctcgaggtcg accagggcgg ggtactggtc ctggtcctcg ggggcgtcga
     4141 gcaggccgag ggcgatgacc cgtccggcat cctccggggc gatctcgtag cgggggtcgg
     4201 ccgccagcgc gtcgagcgtg acgtggtcgg gcagcatctc ccgccagggg ggccggctcg
     4261 ccgggagccc ggtacgggcg tgggccgcga tcacggcgtc gagcaccgcc tcgaggtggg
     4321 tggtgggtcc gctggcggac gcgtcggcac cgccgtcggc ggcggactgg ctggccaggg
     4381 gggcgccgac gccggtggtg tcgaagctgc gcacgaccac cacgccgcgc tgggcgtccc
     4441 cgtcgagggg cgcgccggcg aaggccgact ggaagggcac caggtcgtgc gggcccagcc
     4501 gggcgtagcc ccggcctctc aggggcacgg ggatgccggc ggcctcgggg ctgccgatga
     4561 cgctgcccga ctccgcgttg tcgagcatgc gcagggcgat gcgcaggttc gtgttggcca
     4621 ggatgttctc gttcaccgac ccggacgggc gctgggtggc gaggatgagg tggatgccga
     4681 ggctgcggcc gcgctgggcg atgtcgacga cgccggcgac gaactcgggc acctccttca
     4741 cgagcgtggc gaactcgtcg acgacgatca ccagcgacgg gggtgcgtcg tcagggtggt
     4801 gctcgagcat ctcggccagg tccttggccc ggccctccat gagccgcatc cgccggtcga
     4861 gctcggctcg cagcgacgtc agggcccggc gggacagggt ggcgtcgagg ttggtgacgt
     4921 agcccaccgt gtgcgggacg tcgcggaaca ccgtggagga ggcgccgccc ttgtagtcca
     4981 cgaacagcag gttcaggcgg ttcggcgggt ggcagctgat cagcgaggcc accatcgact
     5041 gcagcagctc ggacttgccg gcgccggacg tgccgccgat gagggcgtgg gggccgtcgg
     5101 ccaccaggtc gagggagaag acgccgtccg gtccgaggcc caccgggtgg gcgaggccgt
     5161 agccggtgtc ggtgcgccag cggcgcagga cccactcggc gtcgggccgg tcggtgccga
     5221 gcaccgacag caggggcacc acccgcggga tcgccgtcgt cgcggtggcc acggaggcgt
     5281 cgcgcacggc ggccaggctg cgggccaccc ggtcggccac gtcggggtgg acctggccga
     5341 gctccacggt ctggtcgtcg acgtcggggt cggtggacca gaggcgggcg aacccgggct
     5401 cctggggcgg gccgcagtcg accaccgagc gggcctggcg ggggacgttg ccctcgctgg
     5461 aggagcacca caggacgctg atgccggcgg cctgggcccg gtcgagcagc tgggacacca
     5521 cggtggcgtc caggccgaga tcaccgtcga gcacggccag cagccacggc cagcgccggt
     5581 cgacccggtt gtcggcttcg gcgaggcgcc ggtcggcgac ctcgaccagg ccgttgagga
     5641 ggcggcgggc gtcgtcggcg tcgacgcaca ggtggctgcc ggccagcggc gacgtcgccg
     5701 agcgcacgtg ggggagccac ttcagccact cggcgaccgg ccggtcggcg ccgaccgcgc
     5761 cgaccaccac caggtcctcg gggctgtgca ggcaggcgac ctgcaccagc acggaggaca
     5821 ccacgtcgtc gacctcctcg ggcggtccgt gcacgccgac gacgccgagg tcggcgaggc
     5881 cgaccgtcac cgggacgtcg ccgatggtgt cgtggccggc gatcaccgcc tccacccgct
     5941 cgcggtagtc gtcgtcgccg ccggaggcga gcgtggcggc gacgagcgac ggtgcgtcgc
     6001 ccaggccgag gcgcacgcgg aggaaatcct cgtcgtggcg gccgcggggc cacaggtcga
     6061 tcgtgcgcag ctcggcgcga cgggcgaggt cggccaggtc cggcgcggcc cggacgcgct
     6121 cgacccgctc ggcctcgcga gcggcggcga tctcggactc gaaggccagg agccgctcct
     6181 cgaagcgctc gacgtcgtcc cggaaccggt ggcggccgct gcggcggtcc tcgatccacg
     6241 tgcccaccat caccagcggc gagatggcga tgatggccag gaactgcacc tgccgggaga
     6301 acgcgaacag cagggcgccg gccgccagcg gggcgaggac ggcgagcagc tggaagcggc
     6361 gcggctcggg acgggcgggg atggggccga acggctcgag ctcgcgctcg cgcaccttgt
     6421 cggggcggta cggggtgcgg tggaactcga tctggccgag gcggtcgatg gcctcgtgct
     6481 cggcccggat gaactcccgg aagctgaccc ggctcgaccc gagcgccacc acgtcgtcgg
     6541 ggccgacgac cgtggcctcg tcgatgggct cgtcgttgac gaggacgccg ttctgggggt
     6601 cctcgtgggg cg
//
LOCUS       DATISU010000152         6193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_7304, whole genome shotgun sequence.
ACCESSION   DATISU010000152 DATISU010000000
VERSION     DATISU010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7304"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VLR27_06230"
     CDS             <1..585
                     /locus_tag="VLR27_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03078.1"
                     /translation="MYGGKPQYEDGTEVEVEAEKSFAKAHYEQHCAVTGTIRVGEETI
                     DMDGLGLRDKSWGPRYWQALSWYRWLPMTFSKDFAMMISIVGGKPGGMVLEGDEYHII
                     RDCSIESDWDEDKYQTAMRCSVTTDHDTYEVTGEVISLIPLRNRRQDPDGNELFTRIT
                     EAMTRFECRGEKGIGMSEYLDQIVDGTPVGDDVR"
     gene            complement(582..1766)
                     /locus_tag="VLR27_06235"
     CDS             complement(582..1766)
                     /locus_tag="VLR27_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP03079.1"
                     /translation="MTGVEGAVAVELHEVWRRYRVASTRRPRGLRAGLSRDPFRRSPD
                     RWHWALRDVSLRVDAGEAVALIGHNGAGKSTLLRLVGGVGRAERGTVRVQGRIGALID
                     LGREFHGELTGRQNAEIAAVVGGWSRRDLRHRLPEILEFAELTDFADEPLRAYSDGMR
                     ARLAFSVMAHLEPDVLLVDEVLAVGDAPFQRRSIERIDELRRHGAAVLFVSHDLGLVR
                     RVCDRAVWLDRGQVRLAGDSRDVVASYLEDVTGARPDEGGTGSSPGALAAMAVLDEWG
                     MPTSAIRAGDGLEVRVGVFVPDGHEQAQLRVRVLGPDGATAVVDTSTPVHAGDASEHA
                     VCFARLDLAPGEHGVEGALFSADWSARLDEAGPTPLLVRGDGPDTAPLAPPHVWRRGG
                     RT"
     gene            complement(1763..2536)
                     /locus_tag="VLR27_06240"
     CDS             complement(1763..2536)
                     /locus_tag="VLR27_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP03080.1"
                     /translation="MSPAPSTARRAPDLAAVLVRRDLRLAYGGAAFGLLWAPATVLVQ
                     TVALAFLFRRVVPLGIDDYPAFLLGGLVVWHLVSTALNGSAGAFRDNADLVRRPGFPD
                     AVLPLVAVGRSLAGYALSLPVVVVALAVAGRVHVTALALPLVVLATLLLLVGPAFVIA
                     TLDVRRRDVGHLVRVGMGVLFYVTPVFYPEERIPDGYAWVSELNPVAGVVTLHRQVLF
                     DGAWPDVGRLATVLAFALVGIGLGAFAYRRAARHLADDL"
     gene            complement(2533..3987)
                     /locus_tag="VLR27_06245"
     CDS             complement(2533..3987)
                     /locus_tag="VLR27_06245"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSP03081.1"
                     /translation="MVVCQDDAPTLTDCLDAIAEQRAPGVEVFVVDRASRDGSADVAI
                     RHPAVDRVIPAARDGLQLVVDLASTEARRLVLIGADRRPRAGWLPAAVDGLRRAGVVH
                     GPDRSLGNVAIDLTRLRHVRLLSGVDTLDGLIERAALAGACATEIAEMVVADAAPAPP
                     PSRPLPVTSRPAPGPRWLGTISVVLCTRDRPAQLRRCLDSLARLDDDDHEILVVDNNG
                     APSLDVDALPPRARLLHEPRRGLDVARNRGATHAVGAVLAYVDDDCEVDPHWLTALRR
                     AFADPAVGLVTGRVRPASLERSTHRSFESYFGFDRGLVRRRFTPWDDRPWYPLWTGLL
                     GTGCNMAMRRSDLDRVGGFDERLDVGTTIGGGGDLDVFARLLQTGTIAEYAPDALVWH
                     HHRDEPRDLARQFRGYGQASGAYLTKVAVEQPGMRGVALRCYVDRVARRLRVARSIRA
                     GTHVISMRLLLTDLVGHLTGPALFLRSGRDPRRS"
     gene            complement(4069..5577)
                     /locus_tag="VLR27_06250"
     CDS             complement(4069..5577)
                     /locus_tag="VLR27_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HSP03082.1"
                     /translation="MTTALGIDVGTTNVKAAIVGADGTVHATASRNLVADVEGARAEL
                     DADAQWQAVVDAVGECVAAAPKASSEIVAVGVDSQYSSIVPVDADGRPVAPMRLWSDR
                     RGTDRSFAILREHEDAFMGFIERHGIPPVGGGLSLGHVLHLQHDCPDVHARTAAYLEP
                     MDHINARLTGRLAANQATQFMSQLVDNRSLGQTAYDDVLLGWSGVDASRLPPLLPMDE
                     PIGVVRPEVAEALGIPATAIVHAGVNDTQAGSIATDASRDGRAGLSIGTTSVLVDAIA
                     TKEVDLDHEVLSCPGPFTDRYLVFAENGLGGKPLEHVLDNVVHTGGFDTLDEVLDRVP
                     AGSGGVLFLPWLRGSMAPANDQHMRGGYVNMSLETGRNELVRATCEGVAHNLAWLLPH
                     AEALTGEPVREIVFVGGAARSAGWAQIVADVLDRPVLVPDRPEVAIARATAMLALHRH
                     GVLDRDDLSRLASSGEALHPIAAHHDVYARHQPQFEAVFEALRPISAALNAS"
     gene            complement(5642..>6193)
                     /locus_tag="VLR27_06255"
     CDS             complement(5642..>6193)
                     /locus_tag="VLR27_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSP03083.1"
                     /translation="DAFVDAFVAEMEAAIVGDPMDEATTVGPQAQVSLRDELHDQVQR
                     SVAQGAHLVLGGMLPSGDGAFYPPTVLTGVTTDMAAGSEETFGPVGAVLRAQDEAHAI
                     ELANDTSFGLGAAVFTQDLERGERIAAEELAAGCCFVNDLVASDPRLPFGGIKDSGYG
                     RELSHLGIREFVNQKTVVVGPKQ"
BASE COUNT          906 a         2215 c         2326 g          746 t
ORIGIN      
        1 atgtacggcg gcaagcccca gtacgaggac ggcaccgagg tcgaggtcga ggccgagaag
       61 tccttcgcca aggcccacta cgagcagcac tgcgccgtca ccggcaccat ccgggtcggc
      121 gaggagacca tcgacatgga cggcctcggc ctgcgggaca agtcgtgggg cccccgctac
      181 tggcaggccc tgtcctggta ccgctggctg ccgatgacct tctcgaagga cttcgccatg
      241 atgatctcca tcgtcggcgg caagcccggc ggcatggtcc tcgagggcga cgagtaccac
      301 atcatccggg actgcagcat cgagtcggac tgggatgagg acaagtacca gacggccatg
      361 cgctgctcgg tcaccaccga ccacgacacc tacgaggtca ccggcgaggt catctccctg
      421 atcccgctgc gcaaccggcg ccaggacccc gacggcaacg agctgttcac ccgcatcacc
      481 gaggccatga cccgcttcga gtgccgggga gagaagggca tcgggatgag cgagtacctg
      541 gaccagatcg tcgacggcac ccccgtcggc gacgacgtcc gctaggtccg gccaccccgg
      601 cgccacacgt gcggtggcgc cagcggtgcg gtgtcggggc cgtcgccccg caccagcaac
      661 ggcgtgggcc cggcctcgtc gagccgcgcc gaccagtcgg cgctgaagag ggccccctcg
      721 accccgtgct ccccgggcgc caggtcgagg cgggcgaagc agaccgcgtg ctccgacgcg
      781 tcgccggcgt gcacgggcgt gctggtgtcc acgacggcgg tcgcgccgtc gggacccagg
      841 acgcggacgc gcagctgcgc ctgttcgtgg ccgtcgggca cgaacacccc gacgcggacc
      901 tccaggccgt cgccggcgcg gatcgccgac gtcggcatgc cccactcgtc gagcaccgcc
      961 atggcggcca gggcgcccgg cgatgacccc gtgccgccct cgtccgggcg cgcacccgtg
     1021 acgtcctcga ggtacgacgc cacgacatcc cggctgtcgc cggcgaggcg gacctgccct
     1081 cggtcgagcc acaccgcgcg gtcgcacacc cgacggacca ggccgaggtc gtgggacacg
     1141 aacagcaccg cggcgccgtg ccggcgcagc tcgtcgatgc gctcgatcga gcgtcgctgg
     1201 aacggggcat cgcccacggc gagcacctcg tccaccagca gcacgtcggg ttcgaggtgg
     1261 gccatgaccg agaaggccag ccgggcgcgc atcccgtcgc tgtaggcccg cagcggctcg
     1321 tcggcgaagt cggtgagctc ggcgaactcg aggatctcgg gtagccggtg ccggaggtcc
     1381 cggcgggacc agccgccgac gaccgcagcg atctcggcgt tctggcggcc ggtcagctcg
     1441 ccgtggaact cccgtccgag gtcgatgagc gcgccgatcc ggccctgcac ccggaccgtg
     1501 ccgcgctcgg cacgcccgac gcccccgacg agacggagca gcgtggactt gccggcgccg
     1561 ttgtgcccga tcagggcgac ggcctcccca gcgtcgaccc ggaggctcac gtcgcggagc
     1621 gcccagtgcc agcggtcggg gctgcgccgg aacgggtccc ggctgaggcc ggcccgcagc
     1681 cctcgtggcc gtcgggtcga ggcgacgcgg taccgccgcc acacctcgtg cagctcgacg
     1741 gcgacggcac cctcgacccc ggtcacaggt cgtcggccag gtgccgggcc gcccgccggt
     1801 aggcgaacgc accgaggccg atgccgacga gagcgaaggc gaggacggtg gcgagccgtc
     1861 cgacgtcggg ccaggcaccg tcgaagagca cctggcggtg gagggtgacg acgccggcga
     1921 ccgggttcag ctcgctcacc catgcgtagc cgtcggggat gcgctcctcc gggtagaaga
     1981 cgggcgtgac gtagaagagc acgcccatcc cgacccgcac gaggtggccg acgtcgcggc
     2041 ggcgcacgtc gagcgtggcg atcacgaacg ccggcccgac caggagcagc agcgtggcca
     2101 gcaccaccag cggcagggcg agggcggtca cgtgcacccg gccggccacg gcgagcgcca
     2161 ccaccacgac ggggagcgac agcgcgtagc cggccagcga tcgacccacg gcgacgagcg
     2221 ggagcaccgc atccgggaag ccgggccggc ggacgaggtc ggcgttgtcg cggaacgcgc
     2281 cggcgctgcc gttgagcgcg gtgctgacca ggtgccacac gaccagcccg ccgagcagga
     2341 acgccgggta gtcgtcgatc ccgagcggga cgacgcgtcg gaagaggaag gcgagcgcca
     2401 ccgtctgcac caggacggtg gcgggtgccc acagcaggcc gaaggcggca ccgccgtagg
     2461 cgagccggag gtcccggcgc accaggaccg ccgccaggtc cggtgcccga cgggcggtgg
     2521 agggtgccgg gctcacgacc ggcgcgggtc ccgaccggac cgcaggaaga gggcgggccc
     2581 cgtcaggtga ccgaccaggt cggtgagcag gagccgcatc gagatcacgt gggtcccggc
     2641 ccggatcgat ctcgccaccc ggagtcgccg cgccacgcgg tccacgtagc agcgcagggc
     2701 gacgccgcgc atccccggct gctcgaccgc gaccttggtc aggtaggcgc cggaggcctg
     2761 accgtagccc cggaactgcc gggcgaggtc acgaggttcg tcccggtggt ggtgccacac
     2821 cagcgcatcg ggggcgtact cggcgatggt gccggtctgc agcaagcggg cgaacacgtc
     2881 gaggtcgccg ccgcccccga tcgtcgtgcc gacgtcgagg cgctcgtcga agccacccac
     2941 ccggtcgagg tcgctgcgcc gcatcgccat gttgcagccg gtgccgagga gcccggtcca
     3001 cagcgggtac cagggccgat cgtcccaggg cgtgaagcgt cgccggacca ggccgcggtc
     3061 gaagccgaag tagctctcga agctgcggtg cgtcgaccgc tcgaggctgg ccggacgcac
     3121 gcgtcccgtg accagcccga cggccggatc ggcgaacgcc cgtcgcaggg cggtgagcca
     3181 gtgggggtcg acctcgcagt cgtcgtccac gtacgccagg acggcgccga cggcatgggt
     3241 cgccccccgg ttgcgggcga cgtcgaggcc gcgccggggc tcgtggagca ggcgggcgcg
     3301 gggtggcagc gcgtcgacgt ccagcgacgg cgcaccgttg ttgtcgacca ccaggatctc
     3361 atggtcgtcg tcgtccaggc gggcgagcga gtccaggcac cggcggagct gcgccgggcg
     3421 gtcccgggtg cacaggacca ccgagatggt gcccaaccac ctcggcccgg gtgccggacg
     3481 ggaggtgacg ggcaggggac gggacggtgg gggcgccggt gcggcgtcgg cgacgaccat
     3541 ctcggcgatc tcggtcgcgc acgcccccgc cagcgccgct cgctcgatga gcccgtcgag
     3601 ggtgtccacg ccggacagca ggcggacgtg gcgcaggcgg gtcaggtcga tggcgacgtt
     3661 gccgagcgac cggtccggtc cgtggaccac gccggcccga cggagcccgt cgaccgcggc
     3721 cggaagccat cccgctcggg gacggcggtc ggctccgatg aggacgagtc gccgcgcctc
     3781 ggtcgaggcg aggtccacga cgagctggag gccgtcgcgg gcggcgggga tcacgcggtc
     3841 gaccgcgggg tggcggatgg cgacgtcggc gctgccgtcg cgggacgccc ggtcgaccac
     3901 gaagacctcc acgccgggtg ctcgctgctc ggcgatggcg tcgaggcagt cggtcagcgt
     3961 gggcgcgtcg tcctggcaca ccaccacgac ccgacacccg atgccagctc cgagcacgcc
     4021 ccgacctccg cccgcacacg ttacgggacg aaacggacga atcgacagtc aggaggcgtt
     4081 gagtgcggcg ctgatcggac gcagcgcctc gaacacggcc tcgaactggg gctggtgccg
     4141 ggcgtagacg tcgtggtggg cggcgatggg gtgcagggcc tcgccgctgc tcgccaggcg
     4201 cgacagatcg tcgcggtcga gcacgccgtg gcggtgcagg gccagcatcg cggtggcccg
     4261 ggcgatggcg acctcgggtc ggtcgggcac gagcaccggg cggtcgagca cgtcggcgac
     4321 gatctgggcc cagcccgcgg accgggccgc cccaccgacg aacacgatct cgcgcaccgg
     4381 ctccccggtg agcgcctcag cgtgggggag cagccaggcc aggttgtggg ccacgccctc
     4441 gcacgtggcc cgcaccagct cgttgcgccc ggtctccagg ctcatgttca cgtagccgcc
     4501 ccgcatgtgc tggtcgttcg ccggtgccat cgagccccgc agccagggca ggaacagcac
     4561 gcctccgctg ccggccggca cccggtcgag cacctcgtcg agggtgtcga agccgccggt
     4621 gtgcacgacg ttgtccagca cgtgctccag cggcttgccg ccgaggccgt tctcggcgaa
     4681 caccaggtag cggtcggtga acggcccggg gcaggacagc acctcgtggt cgaggtcgac
     4741 ctccttggtg gcgatggcgt cgaccaggac gctggtggtg ccgatgctga gcccggctcg
     4801 tccgtcgcgg gaggcgtcgg tggcgatgga gccggcctgg gtgtcgttca cgccggcgtg
     4861 gacgatggcg gtggccggga tcccgagggc ctcggccacc tcgggccgga cgaccccgat
     4921 cggctcgtcc atcggcagga gcggcgggag cctcgacgcg tccacgcccg accacccgag
     4981 cagcacgtcg tcgtaggcgg tctggccgag cgagcggttg tccaccagct ggctcatgaa
     5041 ctgcgtggcc tggttcgcag cgaggcggcc ggtgagccgg gcgttgatgt ggtccatcgg
     5101 ctccaggtag gccgcggtgc gagcgtgcac gtccgggcag tcgtgctgga ggtgcaggac
     5161 gtggccgagg ctgagtccgc caccgaccgg cgggatgccg tggcgctcga tgaagcccat
     5221 gaacgcgtcc tcgtgctcgc ggaggatcgc gaagctccgg tcggtgccgc gccggtccga
     5281 ccagagccgc atcggcgcca ccggccgacc gtcggcgtcg accggcacga tcgaggagta
     5341 ctggctgtcg acgccgacgg ccacgatctc gctcgacgcc ttcggtgcgg ccgccacgca
     5401 ctcccccacg gcgtcgacca ccgcctgcca ctgggcgtcc gcgtcgagct cggcccgggc
     5461 accctcgacg tcggcgacga ggttgcggct cgcggtggcg tgcacggtgc cgtcggcgcc
     5521 gacgatggcc gccttgacgt tcgtggtgcc gacgtcgatg cccagagccg tggtcacgcc
     5581 cgacatccaa cccgaaaatg ccgtcggccg gtcacacctg cggtggccga gcgacgacag
     5641 atcactgctt cggaccgacg accaccgtct tctggttgac gaactcccgg atcccgaggt
     5701 ggctgagctc gcggccgtag cccgagtcct tgatgccgcc gaagggcagt cgggggtcgg
     5761 aggcgacgag gtcgttgacg aagcagcagc cggccgcgag ctcctcggcg gcgatgcgct
     5821 cgccacgctc gaggtcctgg gtgaacacgg cggcgccgag cccgaacgag gtgtcgttgg
     5881 ccagctcgat ggcgtgggcc tcgtcctgcg cccgcagcac cgcgcccacc gggccgaagg
     5941 tctcctccga cccggccgcc atgtcggtgg tgacgccggt gagcacggtg ggcgggtaga
     6001 acgcgccgtc gcccgatggg agcatgccgc cgaggacgag gtgggcaccc tgggccaccg
     6061 accgctggac ctggtcgtgc agctcgtcgc gcagcgacac ctgggcctgc ggaccgaccg
     6121 tcgtggcctc gtccatgggg tcgccgacga tcgcggcctc catctcggcc acgaacgcgt
     6181 cgacgaaggc atc
//
LOCUS       DATISU010000153         5050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_659073, whole genome shotgun sequence.
ACCESSION   DATISU010000153 DATISU010000000
VERSION     DATISU010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659073"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="VLR27_06260"
     CDS             <1..1166
                     /locus_tag="VLR27_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03084.1"
                     /translation="TSKGRTTLALGLAGALVIGGVAAAGGSSPGPSPAPVERVQMVAE
                     IEPGDIRSGDEVLHRDEPATVTPDRSLAAGRNQLDETSPWSGLWGPEIEPLAAYEPQE
                     RCLSGARPAVAAFAQLLEQAYPQGRDLGISRACEAGSRSEHKEGRAYDWGVRVDVPTQ
                     RMAAEELVAWLLATDEHGNDFAMARRLGVMYVIWDGHIWSAGAAGDGWRVYRGPSPHT
                     DHVHLSFSWAGAIGTTSFWDAARLGPWLFGHVAVDRLPSSFDLGRDRYRAELGDAPGS
                     LHTWGTPPDDPGEEQDTPATEEPAAADAPAPAAPEPDPAPSSAPPAPSEPIASTSGDA
                     LLGPVLEAPVLDPVVPELPTPPTTTVPLVEDVGEVVDEVSDEVTDTADDLLGG"
     gene            complement(1171..1647)
                     /locus_tag="VLR27_06265"
     CDS             complement(1171..1647)
                     /locus_tag="VLR27_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03085.1"
                     /translation="MWLLPMSLRAKMATVILLGIFFIPFATSDLRGLTHVLTCTDAVS
                     ATLLVDDSADDDTSPVLGSADTVTRDEVGLGLCGGLEVDLQLTSARDGRAEVLVAVTN
                     ATEHDWKGSIELRFDGTPVPVAIGSIAQGDTEQDTVELRVKGGEAYEISGTLLIGP"
     gene            1710..2570
                     /locus_tag="VLR27_06270"
     CDS             1710..2570
                     /locus_tag="VLR27_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03086.1"
                     /translation="MATQDMSARLARMGYGRARLMLSLVGLAILAVLVAVLLVRSVDT
                     TEVVATLLFAPIFLLLLWFRLPGGLIGAAAATGVYVVLRADAIDAVGWGEFSALIVTR
                     AIAYFLFGAVGGWASGTLSMSLDKLDLYDQVDDLTGVNNARFLLTDIDLEKARSQRYQ
                     TVFSVSFLAIDAAPIGDLPARKRKALLRQLGAKLDDGIRTMDRVAHGYDGSCHRIATI
                     LPETAGEGARVFQVRLEEQIRAFLAEHGAAGATVDGEIVTVPGDDDALERHLAEWKAI
                     DQAEHAAPRT"
     gene            2703..>5050
                     /locus_tag="VLR27_06275"
     CDS             2703..>5050
                     /locus_tag="VLR27_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HSP03087.1"
                     /translation="MADSFTHLHVHTEYSMLDGAARLDELVAAAAADGQPALGITDHG
                     NMYGILDFYRACKDQGVKPILGTEAYMAHDHRTERIAARGKMDDSGGEGEGGRKPWYH
                     LTLLAESEVGYKNLIQLASRSFMEGYYRKPKIDWELLEEHSEGLIATTGCLGGHVLQA
                     MVPKAIMDPDKQQADPLSEDERFDNAVKLAGRLQDIFGRDNLFVELQDHGIPAQQWSN
                     PRLAQLAKAIRAPLLATNDSHYVHRGDALAHDALLCQPPGTLVRRVIGRSRTPAGEPW
                     SYSYEDVPIEEIREGDKVLSWSGKTRRGQMHKKGSPVTKVGIRSYDGKLVRVTTERGR
                     MSRYTQDHICIARTDVALPQGNHIVYLMRRGQSFRLGATTHKREFNNKPLGAIVRAKE
                     QGCDDIWILSVHGSRSAALMAEALLSFEYGIPTWQFRSTSNQWVDLDGFWEKVGDLTD
                     RARRCLEDHHRLLEYPFWSAGESGSSRRPKEIRACNLLDGMLVCEPDECTDQHLATYH
                     GSNAWSRVSVAVEPYAGVVHSIDVDGDHTYVADGIVTHNCVQTNSLVSDKNRFKFHGD
                     DHYLKSAEEMRYLFRELPTACDNTLWVAERCEVEIEFGKPQLPNFPLPPGFATDDDYL
                     RHLVFEGAKKRWGDTLDDKVTDRLLFELKVIGDMGFSSYFLITWDLIKHARDNEIRVG
                     PGRGSAAGCAVAYTLWITDLDPIKYDLLFERFLNPSRISMPDIDMDFDTRYRDEMIRY
                     AAERYGRENVAQIITFGTIKARQAVRDAARVLGHDYGTGDRIA"
BASE COUNT          801 a         1836 c         1689 g          724 t
ORIGIN      
        1 cgacgtcgaa gggccgcacc acgctggcgc tcggcctcgc cggcgcgctc gtcatcggcg
       61 gggtggcggc ggcgggcggg tcgtcaccgg gcccgtcccc ggcaccggtc gagcgggtgc
      121 agatggtggc cgagatcgag ccgggtgaca tccgatcggg cgacgaggtc ctccatcgcg
      181 acgagccggc gacggtgacc cccgatcggt cgctggccgc ggggcggaac cagctggacg
      241 agacgtcgcc gtggtcaggc ctgtggggtc ccgagatcga gcccctcgcc gcctatgagc
      301 cccaggagcg gtgcctctcc ggcgcgcgcc cggcggtcgc ggccttcgcc cagctgctgg
      361 agcaggcgta cccccagggc cgggacctgg ggatctcgcg ggcgtgcgag gccggatccc
      421 ggtccgagca caaggagggt cgggcctacg actggggcgt gcgggtcgac gtgccgaccc
      481 agcgcatggc cgccgaggag ctcgtcgcct ggctgctggc caccgacgag cacggcaacg
      541 acttcgccat ggcccgccgg ctcggcgtga tgtacgtcat ctgggacggc cacatctggt
      601 cggccggcgc ggcaggggac gggtggcgcg tctaccgcgg gcccagcccc cacaccgacc
      661 acgtccacct gagcttcagc tgggccggcg ccatcggcac gaccagcttc tgggacgcgg
      721 cccgcctggg accgtggctg ttcggccacg tcgccgtcga tcgcctcccg tcctccttcg
      781 acctcgggcg cgaccgctac cgggccgagc tcggtgacgc ccccggcagc ctccacacgt
      841 ggggcacgcc gccggacgac ccgggcgagg agcaggacac cccggcgacc gaggagccgg
      901 ccgcagccga cgcgcccgca cccgcagcac ccgagcccga ccccgcgccg tcgagcgcgc
      961 cgccggcacc gtcggagccg atcgcctcga cctcgggcga cgccctgctg ggacccgtcc
     1021 tggaggcacc ggtgctcgat ccggtcgtgc cggagctccc gacaccgccg accacgacgg
     1081 tgccgctggt cgaggacgtc ggtgaggtcg tggacgaggt gtccgacgag gtcaccgaca
     1141 ccgccgacga cctgctcggc ggctgaggcc ctacgggccg atcagcaggg tgcccgagat
     1201 ctcgtaggcc tccccgccct tcacgcgcag ctccacggtg tcctgctcgg tgtcgccctg
     1261 ggcgatgctg ccgatggcca ccggcaccgg ggtgccgtcg aagcgcagct cgatgctgcc
     1321 cttccagtcg tgctcggtgg cgttggtgac ggccacgagg acctcggccc ggccgtcccg
     1381 cgccgaggtg agctggaggt cgacctcgag gccgccgcac aggcccagcc cgacctcgtc
     1441 acgggtgacg gtgtcggccg aaccgagcac gggggaggtg tcgtcatcgg cggagtcgtc
     1501 gacgagcagc gtggccgaca ccgcgtcggt gcaggtgagc acgtgggtga ggccgcgcag
     1561 gtccgaggtg gcgaagggga tgaagaagat cccgagcagg atcaccgtcg ccatcttcgc
     1621 ccggagcgac atcgggagca gccacacgcg cgtcatggaa ccacatgagg gtcatccagt
     1681 agcggacggg gtccgggtac tgtccggcca tggcgaccca ggacatgtcg gcgcggctgg
     1741 cccgcatggg ctacggccgc gcccgcctca tgctcagcct cgtcggcttg gccatcctgg
     1801 ccgtcctcgt cgccgtgctg ctcgtgcgca gcgtcgacac caccgaggtg gtcgcgacac
     1861 tgctgttcgc gccgatcttc ctcctcctgc tgtggttccg cctgcccggc ggcctcatcg
     1921 gtgcggccgc cgccaccggc gtctacgtcg tcctgcgcgc cgacgcgatc gacgccgtgg
     1981 gatggggcga gttctccgcc ctcatcgtca cccgggccat cgcctacttc ctgttcggtg
     2041 ccgtgggcgg gtgggcgtcg gggacgctgt cgatgtcgct cgacaagctc gacctgtacg
     2101 accaggtcga cgacctgacc ggcgtcaaca acgcccggtt cctgctcacc gacatcgacc
     2161 tggagaaggc gcgctcgcag cggtaccaga cggtcttctc cgtgtcgttc ctcgccatcg
     2221 acgccgcccc catcggcgac ctccctgctc gcaagcgcaa ggccctgctg cgccagctcg
     2281 gcgccaagct cgacgacggc atccgcacga tggaccgcgt ggcccacggc tacgacggct
     2341 cctgccaccg gatcgccacc atcctcccgg agaccgcagg ggagggcgca cgcgtcttcc
     2401 aggtccgcct cgaggagcag atccgcgcct tcctcgccga gcacggcgcc gccggggcca
     2461 cggtcgacgg tgagatcgtc acggtgccgg gcgacgacga cgccctggag cgccacctcg
     2521 ccgagtggaa ggccatcgac caggccgagc acgccgcgcc tcggacctga cctcggcccg
     2581 ccgaccgtcg gtgggcccct gctacggttc gctcccacga atcacaccgt tgtgattcgt
     2641 ccacagctgt ggacagcggc tgtggatgtc cctgtggaag ccgtcgtctc gaaggggccc
     2701 cggtggcgga ttcgttcacc cacctccacg tccacaccga gtactccatg ctcgacggcg
     2761 cggcgcgcct cgacgagctg gtggcggcag ccgcagccga cggccagccc gccctcggga
     2821 tcaccgacca cggcaacatg tacgggatcc tggacttcta ccgggcgtgc aaggaccagg
     2881 gcgtcaagcc gatcctcggc accgaggcct acatggccca cgaccaccgc accgagcgca
     2941 tcgcggcccg cgggaagatg gacgactccg gcggcgaggg cgagggcggc aggaagccct
     3001 ggtaccacct cacgctgctc gctgagagcg aggtcggcta caagaacctg atccagctgg
     3061 cgtcgcgctc gttcatggag ggctactacc gcaagcccaa gatcgactgg gagctgctcg
     3121 aggagcacag cgagggcctc atcgccacca ccggctgcct cggcggccac gtgctccagg
     3181 ccatggtccc caaggcgatc atggatccgg acaagcagca ggccgatccg ctgtccgagg
     3241 acgagcgctt cgacaacgcc gtgaagctgg ctggccgcct gcaggacatc ttcgggcggg
     3301 acaacctctt cgtcgagctc caggaccacg gcatccccgc ccagcagtgg tccaaccccc
     3361 gcctggccca gctggccaag gccatccggg caccgctgct cgccaccaac gacagccact
     3421 acgtccaccg tggagacgct ctggcccacg atgccctctt gtgtcagccg ccagggactt
     3481 tggttcgtcg ggtcatcggt cggtctcgaa cgcccgccgg cgagccatgg agctacagct
     3541 acgaggacgt gccgattgag gagatccggg agggtgacaa ggtcctctcg tggtcaggaa
     3601 agacccgtcg cggccagatg cacaagaagg gctcgccggt cacaaaggtc ggtatccgct
     3661 cctacgacgg caagctcgtg cgcgtcacga ccgagcgggg ccggatgtcc cgttacaccc
     3721 aggatcacat ctgcatcgct cggacggacg tcgcacttcc gcagggcaac cacatcgtct
     3781 acctgatgcg tcgagggcag tccttccggc tgggagcgac gacacacaag cgagagttca
     3841 acaacaagcc tctgggagcg atcgtccgcg ccaaggagca gggctgcgat gacatctgga
     3901 tcctcagcgt tcacgggagc cggagtgcgg cgctcatggc tgaggcgctg ctgtcgttcg
     3961 agtacggcat cccgacttgg cagtttcggt cgacctcgaa ccaatgggtc gatctggacg
     4021 gcttctggga gaaggtcgga gacctcaccg accgtgcccg ccgatgcctc gaggaccacc
     4081 atcgcctcct cgagtacccg ttctggtcag cgggggagtc gggctcgtcg cgccgaccga
     4141 aggagatccg ggcctgcaac ctcctcgacg gcatgctcgt ctgcgagccg gacgagtgca
     4201 ctgaccagca ccttgcgacc taccacggat cgaatgcctg gagtcgggtc tcggtagcgg
     4261 tcgagccgta cgcaggtgtg gtccacagca tcgacgtcga cggagaccac acctacgtcg
     4321 ccgacgggat cgtcacgcac aactgcgtcc agacgaactc cctcgtcagc gacaagaacc
     4381 gcttcaagtt ccacggcgac gaccactacc tgaagtccgc cgaggaaatg cggtacctct
     4441 tccgtgagct cccgacggcg tgcgacaaca cgttgtgggt ggccgagcgg tgcgaggtcg
     4501 agatcgagtt cggcaagccc cagctgccca acttcccgct gccgcccggc ttcgccaccg
     4561 acgacgacta cctgcgccac ctcgtgttcg agggagccaa gaagcgctgg ggcgacacgc
     4621 tcgacgacaa ggtcaccgac cgcctgctct tcgagctcaa ggtcatcggc gacatggggt
     4681 tctccagcta cttcctcatc acgtgggacc tcatcaagca cgcccgcgac aacgagatcc
     4741 gggtcggtcc gggccgcggg tccgccgccg ggtgcgcggt ggcctacacg ctctggatca
     4801 ccgacctcga ccccatcaag tacgacctgc tgttcgagcg gttcctcaac ccgagccgca
     4861 tctccatgcc cgacatcgac atggacttcg acacccgcta ccgggacgag atgatccgct
     4921 acgcggccga gcgctacggc cgcgagaacg tcgcccagat catcaccttc ggcaccatca
     4981 aggcccgcca ggccgtgcgc gacgccgccc gggtgctcgg ccacgactac ggcaccggcg
     5041 accgcatcgc
//
LOCUS       DATISU010000154         8727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_705905, whole genome shotgun sequence.
ACCESSION   DATISU010000154 DATISU010000000
VERSION     DATISU010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8727
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705905"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VLR27_06280"
     CDS             <1..673
                     /locus_tag="VLR27_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSP03088.1"
                     /translation="KTTLMDCISGFLAIEDGRIGIQGLDVTDWQPHERARAGLGRSFQ
                     DARLYPSLTVAETIAVARERHLASHDIMASTLGLPASYEVEMDIAMKVEELIDLMGLG
                     AYREKLTGELSTGSRRIVDLACILAQGPSVLMLDEPSGGVAQKETEALGPLLLRVRER
                     TGCSILVIEHDMPLLSSICDKLIALETGAIIATGTPDEVLSHPAVIESYLGTDDSAIN
                     RSGTA"
     gene            695..1606
                     /gene="folP"
                     /locus_tag="VLR27_06285"
     CDS             695..1606
                     /gene="folP"
                     /locus_tag="VLR27_06285"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HSP03089.1"
                     /translation="MDPTFRSRDRTIRIDGPAAMGIVNASPESFSGDGGTTRDEQVAQ
                     VRAQFDAGALLVDLGGQSANTKTPELEVEEETARLVPLIEAVRAAGIDGLLSIDTYKP
                     AVADACLRAGADVINDVSALHHPELADVVAEWGAGFVLMHTVGPPKVKILEGDLYGDV
                     VEDVVDFGHRTIDRLAAAGVSREQVLFDPGVDFAKTPRQSLDLVHGIARMAELGRPLL
                     LALSRKDFIGAITATTPRRRDAGTLAAIGAVAAAVPETVFRVHDVPATVQFLAVQQAI
                     ADPDRLPVDAVLADDLRREPVPAGESG"
     gene            complement(2163..2474)
                     /locus_tag="VLR27_06290"
     CDS             complement(2163..2474)
                     /locus_tag="VLR27_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03090.1"
                     /translation="MRPRPLVLVLALALAGCGDEEPSAEERRGAVVDQLAAGLRTEVS
                     TLTEGEANCVAEALVDTVGEERFDDLIAAAADDGDPELRDQVVDVFASCDALDPVRDG
                     G"
     gene            2523..3245
                     /locus_tag="VLR27_06295"
     CDS             2523..3245
                     /locus_tag="VLR27_06295"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HSP03091.1"
                     /translation="MIRTERHDDVLVCTIDRPDRRNAVDAEHLDGLREAFEAVGDARA
                     LVLAGAGSAFCAGADLTHVEDREAAALVRSTLDALSGLPICTIAAVHGPAMGAGMQLA
                     LSCDLRVVGPDARFGIPSAKLGLMVDHWTIEKLALLAGHGPARAVLLAADTIDADAAL
                     RTGFAQRAGDTAAAIAWAAEIATLAPLSVAGQKLALDRLSGREVSDPGVEAAFDRVWT
                     SEDRLEGIAAFQEKRTPRFRGA"
     gene            complement(3249..3767)
                     /locus_tag="VLR27_06300"
     CDS             complement(3249..3767)
                     /locus_tag="VLR27_06300"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HSP03092.1"
                     /translation="MPASHRRAVALGAVALGAALLLGACEGDDGGASGAPASSTPATV
                     QEGPVAATAVAVGDCLNGVVIGAAERATISSAEVVSCDRAHGLEVYATFTLQPDDFEL
                     ADPAEYPGPARIVRAADQRCAERIEDLVDDPATFGLIALWPSQESWAAGDRAVACAVF
                     SPDGSVFEARQI"
     gene            complement(3786..4757)
                     /locus_tag="VLR27_06305"
     CDS             complement(3786..4757)
                     /locus_tag="VLR27_06305"
                     /inference="COORDINATES: protein motif:HMM:NF033747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HSP03093.1"
                     /translation="MRLAKVLSGVGRILIWSGAIVLLFVAYQLWGTGLAHAQAQDDLA
                     DDFAALLDTTTTTTSTTSTTEPEDEPATTTTTAPLPPPPDDGEAVARMVIPKIGVDEI
                     VVEGVSRSDLKKGPGHYPQTPMPGQPGNSAIAGHRTTYGQPFHRVDELVPGDEIIVTT
                     LQGEFRYEVSGTEIVLPSAVEVIDDQGDDRLTLTSCHPKYSARQRIIVSALLVDEPAP
                     ATPTRPEVEAPEELADEDVDEVTDDDDETEEAGVPVDLESLDGVDERTAGPAVRWGLV
                     TAAVWLAFYALSRLWRRWPAYVLGAPVFAVSLFVFFGAFSELLPANY"
     gene            4828..5532
                     /gene="ccmA"
                     /locus_tag="VLR27_06310"
     CDS             4828..5532
                     /gene="ccmA"
                     /locus_tag="VLR27_06310"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HSP03094.1"
                     /translation="MADARPPAVGSAVRMRDAVVLLDRFPALAGVDLDVARGEIVLLR
                     GPNGAGKTTLLRTCAGLARVVQGAVEVLGEDLVSSPRSVRRRVGMLSHQDGLYADLTV
                     EDNVRFWARAAGADLADAGAALARCELDGRLATVPAARLSAGQKRRTALAALLARRPE
                     LWLLDEPHAGLDRAGRDLVDRLVRDAAAAGATVLFASHELDRAEGLATRTVTIAGGQI
                     REDSGATGPIGVERAG"
     gene            5522..6190
                     /locus_tag="VLR27_06315"
     CDS             5522..6190
                     /locus_tag="VLR27_06315"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HSP03095.1"
                     /translation="MLADARLVAGKDLRIEWRSRVGIGQMVPFAVLVLVLFAFALDPD
                     RGILTRATAGLYWVATLFAGLLAVQRAFALESAPGVREAMRLSGLDPAAWFLGKAAAI
                     AVQLLLLEAVLGVGVVVLYSADLQGPLLLLATCVAATVGIAAAGTLYGVLAAGLAVRD
                     TLVPLLLLPVLAPVLLGATRASEAALGNGVGDGWSWCGLLAVFAVLYTMLGMTFFGTL
                     LEET"
     gene            6187..6972
                     /gene="ccsA"
                     /locus_tag="VLR27_06320"
     CDS             6187..6972
                     /gene="ccsA"
                     /locus_tag="VLR27_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HSP03096.1"
                     /translation="MTTTPTEASPAAAPATSTASPLTRVLGTMSVVGLLVAGWLGLFA
                     SPRDVVQKDSVRLMYVHVPAVTWMYVAVIVCALASFMWLRKRTDGWDVLAGAAAEIAT
                     LLTGLGLFTGSVWGRPTWGVYWVWDARLTSTAILFVLLLGYQALRRYPAEPQSRSRMA
                     AVLGLLLVPNAAIVHFSVDWWRSLHQDATVSRIDVSIEGLMLFTLAVSFVVMGIVCAW
                     LLVHRFRVGWLERQAERHHLDAALADRRADADPSAGVAVGGPR"
     gene            6972..7130
                     /locus_tag="VLR27_06325"
     CDS             6972..7130
                     /locus_tag="VLR27_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03097.1"
                     /translation="MLTSTVLAMTHAGYVAAGWIATVGLLGGYAAATIRKGRRLSQVV
                     PPEERRWT"
     gene            7205..7681
                     /locus_tag="VLR27_06330"
     CDS             7205..7681
                     /locus_tag="VLR27_06330"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HSP03098.1"
                     /translation="MTPRQAPPVRGGSADRGKRVAILGTLGVLVVALGFMAYQGLNNA
                     TVFFRNVDEAVEQRDELGDKRFRLQGSVVDGSVEPDGSSVRFAVEYGGVQADVVHLGD
                     PPELFQPDIPVVLEGAWSDEGDWFASDRILVKHTEDYEADNPDRTDDYVGEGTSLE"
     gene            7678..>8727
                     /gene="ccsA"
                     /locus_tag="VLR27_06335"
     CDS             7678..>8727
                     /gene="ccsA"
                     /locus_tag="VLR27_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HSP03099.1"
                     /translation="MNAALGTAGVTLGLIACVAGAVTMVVGLRGERAALIRTGRTYAF
                     LALAGAILATIAMERALITRDFSLEFVANNGSSRTPALFNVATMWAALEGSILLWALV
                     LAGYTALIAHKFRARLTDPLVGWALLTMLVVGIFFFGLMLGPANPFTTVSPVPLDGPG
                     PNPLLQNHILMAFHPPMLYLGYVGFTVPFAFAIAALVTGRVGEGWLLATRRWTLFAWG
                     FLTVGIMLGAWWSYEVLGWGGYWAWDPVENASLLPWLTGTAYLHSVMVQERRGMLRVW
                     NLSLLCATFALPILGTFLTRSGVIDSVHAFSESDIGGWLLGFFGLIVVVTVGLIGWRG
                     DRLRAPGSMDSPISRE"
BASE COUNT         1095 a         3323 c         3060 g         1249 t
ORIGIN      
        1 caagaccacc ctcatggact gcatctcggg cttcctcgcc atcgaggacg gccgcatcgg
       61 catccagggc ctcgacgtca ccgactggca gccccacgag cgggcccggg ccggcctcgg
      121 ccgctccttc caggacgccc gcctgtaccc gtcgctcacc gtggccgaga cgatcgccgt
      181 ggcccgcgag cgccacctgg ccagccacga catcatggcc tcgacgctcg gcctgcccgc
      241 ctcctacgag gtcgagatgg acatcgccat gaaggtggag gagctcatcg acctgatggg
      301 cctcggcgcc taccgcgaga agctcaccgg cgagctgtcc accggctcac gtcgcatcgt
      361 cgacctggcc tgcatcctcg cccagggccc gtcggtgctc atgctcgacg agccctccgg
      421 cggcgtggcc cagaaggaga ccgaggcgct cggtccgctg ctgctccggg tccgcgagcg
      481 caccggctgc tcgatcctgg tgatcgagca cgacatgccg ctgctgtcca gcatctgcga
      541 caagctcatc gccctcgaga ccggtgccat catcgccacc ggcacccccg acgaggtcct
      601 gtcccacccg gccgtgatcg agtcctacct gggcaccgac gactccgcca tcaaccgttc
      661 cggtactgcg tgatctgtcc ctagggtcgg tccgatggac ccgaccttcc gatcccgcga
      721 ccggacgatc cgcatcgacg ggccggcggc gatgggcatc gtcaacgcca gccccgagtc
      781 cttctccggc gacgggggca ccacccgcga cgagcaggtc gcccaggtgc gagcccagtt
      841 cgacgccggc gccctcctcg tcgatctcgg cgggcagtcg gcgaacacca agacgcccga
      901 gctggaggtc gaggaggaga ccgcccggct cgtcccgctg atcgaggccg tgcgggccgc
      961 gggcatcgac gggctcctgt ccatcgacac ctacaagccg gccgtcgccg acgcctgcct
     1021 gcgggccggt gccgacgtca tcaacgacgt ctccgccctg caccaccccg agctggccga
     1081 cgtggtcgcc gagtggggcg cgggcttcgt gctgatgcac accgtcgggc cgccgaaggt
     1141 caagatcctc gagggcgacc tgtacggcga cgtcgtggag gacgtggtcg acttcgggca
     1201 ccggaccatc gaccgcctgg cggcggccgg ggtgtcacgt gagcaggtgc tcttcgaccc
     1261 gggcgtcgac ttcgccaaga cgccccgcca gtccctcgac ctcgtccacg gcatcgcccg
     1321 gatggccgag ctcggccggc cgctgctgct ggcgctgtcc cgcaaggact tcatcggcgc
     1381 gatcacggcc acgacccctc gccggcgcga cgccggcacg ctcgccgcga tcggtgcggt
     1441 cgcggcggcg gtgcccgaga ccgtgttccg ggtccacgac gtcccggcca ccgtgcagtt
     1501 cctcgccgtc cagcaggcca tcgccgaccc agatcggctc cccgtcgacg ccgtgctcgc
     1561 cgacgacctg cgtcgcgaac cggtgccggc gggcgagtcg ggctgatctg gctcggggcc
     1621 ggcgggcgta gcggaccagg tcggccgcca ccgcggcgat cccggccagc acgatcaggt
     1681 cgccgaagga catcaccgca cccagcgcct cgacgggcag gacgtcgccg agcacgggca
     1741 accagtcgcc agtcgcctcg aaccggcgtc cggcgccgag gtcgaccagg acgagctcgc
     1801 cggggcgggc gacgccggcg cggaccaccg cgctggcccg gacgggcatg gccccgttga
     1861 ccagcaccac ggcggcgttc agggccagac cgacgctgag cacccccatg ccgatgaggt
     1921 gcaggttgag gagggcgaag ccggtgagca ggaacaggga cagcccgagg aggggaccgg
     1981 cgaccgcgct gccgagcaga ccgagcgaga ggtgcacgac gacggcgacg acggcgatgc
     2041 cgggagcgac cacccgcggg tcggccaggt gggcgccgag gggccggcgg agcgcaccga
     2101 cgccgacgcc gaccacggcg gcgagcaaca cggacagcac ggcccaaacg gtaacccctg
     2161 gttcaaccgc cgtcccggac cgggtcgagc gcgtcgcagg acgcgaagac gtcgacgacc
     2221 tggtcccgga gctcggggtc gccgtcatct gcagcggctg cgatgaggtc gtcgaagcgc
     2281 tcctcgccga cggtgtcgac gagcgcctcg gcgacgcagt tcgcctctcc ctccgtgagc
     2341 gtgctcacct cggtccgcag gccggcggcg agctggtcca cgactgcgcc ccggcgctcc
     2401 tcggcgctgg gctcctcgtc accgcatccg gccagcgcca gcgccaggac gagcacgagg
     2461 ggccggggtc gcacccggcg agggtatccg ggcaccccgg gggcacctgc caagatgcgg
     2521 cgatgatccg gaccgagcga cacgacgacg tgctcgtctg caccatcgac cggcccgacc
     2581 gccgcaacgc cgtcgacgcc gagcacctcg acgggctgcg cgaggcgttc gaggccgtcg
     2641 gcgatgcccg cgccctggtc ctcgccggcg ctggctcggc cttctgcgcc ggcgctgacc
     2701 tcacccacgt cgaggaccgg gaggctgccg ccctggtccg ctcgacgctg gatgcgctca
     2761 gcggcctgcc gatctgcacc atcgccgccg tgcacggacc ggccatgggc gccggcatgc
     2821 agctggccct gtcgtgcgac ctgcgggtgg tcggaccgga tgcccggttc gggatcccgt
     2881 cggcgaagct cggcttgatg gtcgaccact ggaccatcga gaagctggcg ctgctcgccg
     2941 gtcacggacc ggcacgagcc gtgctgctgg cggccgacac catcgacgcc gacgccgccc
     3001 tgcgcaccgg gttcgcccag cgggcgggag acaccgcagc ggcgatcgcc tgggccgccg
     3061 agatcgcgac cctggcgccg ctgtcggtcg ccgggcagaa gctggccctc gaccggctct
     3121 ccgggcgcga ggtctcggat cccggcgtcg aggccgcctt cgaccgggtg tggacgagcg
     3181 aggaccgcct cgagggcatc gccgccttcc aggagaagcg caccccccga ttccggggcg
     3241 cgtagccctc agatctggcg ggcctcgaag acggagccgt cgggtgagaa caccgcgcac
     3301 gccaccgccc ggtccccggc ggcccaggac tcctgggagg gccacagcgc gatgaggccg
     3361 aaggtggccg gatcgtcgac caggtcctcg atccgctcgg cgcagcgctg gtcggcggct
     3421 cggacgatcc gggccggccc ggggtactcg gccgggtcgg ccagctcgaa gtcgtccggc
     3481 tggagcgtga aggtggcgta gacctccagg ccatgggccc ggtcgcagga gacgacctcg
     3541 gccgagctga tcgtggcccg ctcggcagca ccgatgacca cgccgttgag gcagtcgccc
     3601 accgccaccg cggtggccgc gaccgggccc tcctgcaccg tcgcaggggt cgagctcgcc
     3661 ggggcgccgg acgcgccgcc gtcgtctccc tcgcacgcgc cgaggagcaa cgccgcaccg
     3721 agcgcgacgg cgccgagcgc gacggcgcgc cggtggctgg ctggcatggc cgggaccgta
     3781 ccgggtcagt agttggcggg caggagctcg gagaaggccc cgaagaacac gaacaggctg
     3841 acggcgaaca ccggcgcgcc cagcacgtag gccggccacc ggcgccacag ccgggacagg
     3901 gcgtagaagg cgagccagac ggcggctgtc accaggcccc agcgcacggc gggtccggcg
     3961 gtgcgctcgt ccacgccgtc gaggctctcg aggtccacgg gcacgcccgc ctcctcggtc
     4021 tcgtcgtcat cgtcggtgac ctcgtcgacg tcctcgtcgg ccagctcctc gggggcctcc
     4081 acctcgggtc gggtcggcgt cgccggggcg ggctcgtcga ccaggagggc ggagacgatg
     4141 atgcgctggc gggccgagta cttggggtgg caggaggtga gggtcaggcg gtcatcgccc
     4201 tggtcgtcga tgacctcgac cgcgctcggc agcacgatct cggtgccgga gacctcgtag
     4261 cggaactcgc cctggagggt ggtgacgatg atctcgtcac cggggaccag ctcgtccacg
     4321 cggtggaacg gctggccgta ggtggtgcgg tgcccggcga tggccgagtt cccgggctgg
     4381 ccgggcatcg gggtctgcgg gtagtggccc gggcccttct tcaggtcgct ccggctcacg
     4441 ccctcgacca cgatctcgtc cacgccgatc ttggggatga ccatccgggc caccgcctca
     4501 ccgtcgtcgg gcggcggcgg cagcggcgcg gtggtggtcg tggtggccgg ttcgtcctcg
     4561 ggctcggtcg tgctggtggt cgacgtcgtc gtggtggtcg tgtccaggag ggcggcgaag
     4621 tcgtcggcca gatcgtcctg ggcctgggcg tgggcgagcc cggtgcccca cagctggtac
     4681 gccacgaaca gcagcacgat cgcgcccgac cagatcagga tccggccgac gccgctgagc
     4741 accttggcga gccgcatcgc cacgagcgta ggggaggcgg cgaggcgtgc cttggtcggc
     4801 ttctcggccc gtcggtaccg ttgggcggtg gccgatgcac gcccccctgc ggtcggctcg
     4861 gcggtgcgca tgcgcgacgc cgtcgtcctg ctcgaccgct tccccgccct cgccggggtg
     4921 gacctggatg tggcgcgggg tgagatcgtg ctgctccggg ggccgaacgg tgccggcaag
     4981 accacgctgc tgcgcacctg cgccggcctg gcccgggtcg tgcagggcgc cgtggaggtg
     5041 ctcggggagg acctggtgtc gtcgccgcgg tcggtccgcc ggcgcgtcgg catgctcagc
     5101 caccaggacg ggctctacgc cgacctcacg gtcgaggaca acgtgcggtt ctgggctcgg
     5161 gccgccggcg ccgacctcgc cgatgccggc gccgcgctcg cccgctgcga gctcgacggc
     5221 cgcctggcca ccgtgccggc cgcccgcctg tccgccggcc agaagcgccg caccgccctc
     5281 gccgccctgc tcgcccgccg gcccgagctg tggctgctgg acgagcccca cgccggactg
     5341 gaccgcgccg ggcgcgacct ggtcgaccgc ctcgtccgag acgcggcggc agccggcgcc
     5401 accgtgctct tcgccagcca cgagctggac cgggccgagg gcctggccac gcggaccgtc
     5461 accatcgccg gcggccagat ccgcgaggac agcggcgcca ccggcccgat cggggtcgag
     5521 cgtgctggct gacgcccgcc tcgtcgccgg caaggacctg cgcatcgagt ggcggtcccg
     5581 cgtcgggatc ggccagatgg tgcccttcgc cgtgctcgtc ctcgtgctct tcgcgttcgc
     5641 cctcgacccc gaccggggca tcctcacccg ggccaccgcc ggcctctact gggtcgccac
     5701 cctcttcgcc gggctgctcg ccgtccagcg ggccttcgca ctcgagtccg cccccggcgt
     5761 ccgggaggcc atgcggctct ccggcctgga cccggccgcc tggttcctgg gcaaggcagc
     5821 cgccatcgcc gtccagctcc tgctgctcga ggccgtgctc ggcgtcggcg tggtggtgct
     5881 gtactcggcc gacctgcagg ggccgctgct cctgctcgcg acctgcgtgg ccgcgaccgt
     5941 ggggatcgcc gccgccggta ccctctacgg cgtacttgct gcgggactgg ccgtgcgtga
     6001 cacgctggta ccgctcctcc tgctcccggt cctcgcaccg gtcctgctcg gggccacccg
     6061 ggcgtcggaa gcggcgctcg gcaacggcgt cggtgatggc tggtcgtggt gcgggctgct
     6121 ggcggtgttc gccgtcctct acacgatgct cggcatgacc ttcttcggca cgctcctgga
     6181 ggagacgtga caaccacccc caccgaggcc tcgccggcgg ctgcgcccgc caccagcacc
     6241 gcctcgccgc tcacccgtgt gctcggcacg atgtccgtcg tcggcctcct ggtcgccggc
     6301 tggctcggtc tgttcgcgtc cccgcgcgac gtcgtgcaga aggactcggt ccgcttgatg
     6361 tacgtgcacg tgccggcggt cacgtggatg tacgtcgccg tcatcgtgtg tgccctcgcc
     6421 tcgttcatgt ggctgcgcaa gcgcaccgac ggctgggacg tcctggccgg cgccgccgcc
     6481 gagatcgcca cgctgctcac cggtctgggg ctcttcaccg gctcggtgtg gggccgcccg
     6541 acctggggcg tctactgggt ctgggacgcc cggctcacct ccaccgccat cctgttcgtg
     6601 ctgctgctcg gctaccaggc cctgcgccgc tacccggccg aaccccagtc gcgctcgcgc
     6661 atggcggccg tgctcggcct gctcctggtg cccaacgcgg ccatcgtcca cttctccgtc
     6721 gactggtggc ggagcctgca ccaggacgcc accgtctccc ggatcgacgt gtccatcgag
     6781 gggctcatgc tgttcacgct ggccgtgagc ttcgtcgtca tgggcatcgt gtgcgcctgg
     6841 ctgctcgtgc accgcttccg ggtcggctgg ctggagcgcc aggccgagcg ccaccacctc
     6901 gacgccgccc tggccgaccg ccgggccgat gccgacccct ccgccggggt cgccgtggga
     6961 ggtccccgct gatgctcacc tcgaccgtcc tcgccatgac ccacgccggc tacgtcgccg
     7021 ccggctggat cgccaccgtc ggcctgctcg gggggtacgc ggccgccacc atccgcaagg
     7081 gccgccggct ctcgcaggtc gtccctcccg aggagcgccg ctggacctga ccccgcccac
     7141 cgaccccacc acgcccgatc cccacgcgcc gttcgaggac accgaagacg tcgacggcat
     7201 cgacctgacc ccccgccagg ccccgcccgt ccgcggcggc tccgccgacc gaggcaagcg
     7261 cgtcgccatc ctcggcaccc tcggagtcct cgtcgtggcg ctgggattca tggcgtacca
     7321 gggcctcaac aacgccaccg tcttcttccg caacgtggac gaggccgtgg agcagcgcga
     7381 cgagctgggc gacaagcgct tccgcctcca gggctccgtc gtcgacggct ccgtggagcc
     7441 cgacggcagc tcggtccgct tcgccgtcga gtacggcggc gtgcaggccg acgtcgtcca
     7501 cctcggcgac ccgcccgagc tcttccagcc cgacatcccg gtggtcctcg agggcgcgtg
     7561 gagcgacgag ggcgactggt tcgccagcga ccgcatcctc gtgaagcaca ccgaggacta
     7621 cgaggccgac aaccccgacc gcaccgacga ctacgtgggc gaagggacct cgctcgagtg
     7681 aacgccgcgc tcgggaccgc cggggtcacc ctcggcctca tcgcatgcgt ggcgggtgcc
     7741 gtcaccatgg tcgtggggct ccgaggcgag cgcgccgcgc tcatccgcac cggtcgcacc
     7801 tacgccttcc tcgccctcgc cggggcgatc ctggccacca tcgccatgga gcgggcgctc
     7861 atcacccgcg acttctccct cgagttcgtg gccaacaacg gctcgagccg cacgccggcg
     7921 ctgttcaacg tggccaccat gtgggcggcg ctcgaggggt cgatcctgct gtgggcgctc
     7981 gtgctcgccg gctacaccgc gctgatcgcc cacaagttcc gggcccgact caccgacccc
     8041 ctcgtcggct gggcgctgct caccatgctg gtcgtgggca tcttcttctt cgggctgatg
     8101 ctcggcccgg ccaacccgtt caccacggtg agcccggtgc ccctcgacgg gcccggcccg
     8161 aacccgctgc tgcagaacca catcctcatg gcgttccacc cgccgatgct ctacctcggc
     8221 tacgtcggct tcaccgtgcc gttcgcgttc gccatcgctg ccctggtcac cggtcgggtg
     8281 ggggagggct ggctgctcgc cacccgacgc tggaccctgt tcgcgtgggg cttcctcacc
     8341 gtcgggatca tgctcggggc ctggtggtcc tacgaggtgc tgggctgggg cggttactgg
     8401 gcgtgggacc cggtggagaa cgcgtccctc ctgccgtggc tcaccggcac cgcctacctg
     8461 cactcggtga tggtgcagga gcgccgcggc atgctccggg tctggaacct gtcgctgctg
     8521 tgcgccacgt tcgccctccc gatcctcggc acgttcctca cccgctccgg cgtcatcgac
     8581 tcggtccacg ccttctccga gtccgacatc ggcgggtggc tgctcggctt cttcggcctc
     8641 atcgtcgtcg tcacggtcgg cctcatcggc tggcgcggcg accgtctccg ggcgcccggc
     8701 agcatggact cgccgatctc gcgcgag
//
LOCUS       DATISU010000155         2679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_53879, whole genome shotgun sequence.
ACCESSION   DATISU010000155 DATISU010000000
VERSION     DATISU010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53879"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VLR27_06340"
     CDS             <1..819
                     /locus_tag="VLR27_06340"
                     /inference="COORDINATES: protein motif:HMM:NF016013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HSP03100.1"
                     /translation="MNAALATATGDRRLLGRTVVRYGAAIAVVVGVVAVLHALLGTDL
                     PTNTMADTANISSVAVLLPLVAGAAGALNLMQAERSSLVSGAATGVLVAAALAPPAGL
                     VGMALVIGEWSMVGSGAFLLALQLLGLNLSGALLFRLYGLRPGSARYGRGTSGVSWAA
                     LGASVLGLVVLVTFQLSSSPTLQRSTVQTEVASQITELVVEDPDADLVEVDARFTRAD
                     IPGQDTLLAVVHVQPTGDADLDELEQRLGSELSAAMTERHDVVALVTVTAIPRP"
     gene            complement(877..1878)
                     /gene="glnII"
                     /locus_tag="VLR27_06345"
     CDS             complement(877..1878)
                     /gene="glnII"
                     /locus_tag="VLR27_06345"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase GlnII"
                     /protein_id="HSP03101.1"
                     /translation="MSIKAEYIWIDGTKPTPLLRSKTKILAEGQEPPVWGFDGSSTNQ
                     AEGHSSDCVLNPVFVCPDPIRGGDSILVLNEVLDIDMKPHVSNTRRATAEVAEKYADQ
                     DMWFGMEQEYTFLEDGHPLGWPRGGFPGPQGPYYCGVGADEVYGRDIVEAHLDACIEA
                     GLKISGINAEVMMGQWEFQIGPAGVVEVGDHMWIARWLLYRIAEDFNVAATIDPKPMP
                     GDWNGAGCHTNFSTELMRNDYDACIAAAEVLGKHHTEHISQYGPGVERRLTGMHETAP
                     WTEYSYGVSNRGASVRIPWQVEVDKKGYIEDRRPNANMDPYIVAKLITEVVCEDAAS"
     gene            2045..>2679
                     /locus_tag="VLR27_06350"
     CDS             2045..>2679
                     /locus_tag="VLR27_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HSP03102.1"
                     /translation="MERQREYVLRTVEERGVRLIRLWFTDVLGQLKSFAISPAELEAA
                     FSEGMHFDGSAIDGFSRVQESDVLALPDPKTFEILPWGPSPEDKNSTAAMFCDISNLD
                     GTPFAGDPRHVLKRNLAAASEKGFTFFVAPEMEFFYFESSGTQGRPPVPLDDGSYFDL
                     TTTDVANDLRRKTIHMLEALGIPVEYSFHEDSPSQHEIDLRYTDALSMADNV"
BASE COUNT          356 a          935 c          936 g          452 t
ORIGIN      
        1 atgaacgcgg cgctcgcgac ggcgaccggt gatcgccggc tgctgggtcg cacggtcgtg
       61 cgctacggcg cggcgatcgc cgtcgtggtc ggggtcgtcg ccgtgctcca cgccctgctc
      121 ggcaccgacc tgccgaccaa cacgatggcc gacaccgcca acatctcctc cgtggcggtc
      181 ctcctgccgc tcgtcgcggg cgccgccggc gcgctgaacc tcatgcaggc ggagcgatcg
      241 agcctggtca gcggcgcggc gaccggcgtg ctcgtggccg ccgcgctggc tccgccggcc
      301 ggcctggtcg gcatggcgct ggtgatcggc gagtggtcga tggtcggctc cggtgcgttc
      361 ctcctcgccc tgcagctgct cggtctgaac ctctcgggtg cgctgctgtt ccgcctctac
      421 gggctccggc ccggctccgc ccgctacggg cgcggcacgt ccggggtgtc gtgggcggcg
      481 ctcggagcct cggtcctcgg cctggtcgtg ctcgtcacct tccagctctc ctcgtcgccg
      541 acgctgcagc gctcgacggt ccagaccgag gtcgcgtcgc agatcaccga gctcgtggtc
      601 gaggacccgg acgcggacct ggtcgaggtg gatgcccgct tcacccgcgc cgacatcccc
      661 ggccaggaca ccctgcttgc cgtcgtgcac gtgcagccca ccggcgacgc cgacctcgac
      721 gagctcgagc agcggctcgg ctccgagctc tcggcggcga tgaccgagcg ccacgacgtg
      781 gtcgcgctcg tcaccgtcac cgcgatcccc cgaccctgag cgcggcggtg cccgggccgg
      841 agcccgggca cctgcagtct cggcggtgtc agccgatcag ctggcggcgt cctcgcagac
      901 gacctcggtg atcagcttgg ccacgatgta ggggtccatg ttggcgttgg gacgacggtc
      961 ctcgatgtag cccttcttgt cgacctcgac ctgccacggg atgcgcaccg aggcgccgcg
     1021 gttggacacg ccgtaggagt actcggtcca cggggcggtc tcgtgcatgc cggtgaggcg
     1081 gcgctcgacg ccggggccgt actggctgat gtgctcggtg tggtgcttgc ccagcacctc
     1141 ggcggcggcg atgcaggcgt cgtagtcgtt gcgcatcagc tcggtggaga agttcgtgtg
     1201 gcagccggcg ccgttccagt cgcccggcat gggcttcggg tcgatggtgg cggccacgtt
     1261 gaagtcctcg gcgatgcggt agaggagcca gcgggcgatc cacatgtggt cgccgacctc
     1321 gacgacgccg gcgggaccga tctggaactc ccactggccc atcatcacct cggcgttgat
     1381 gcccgagatc ttcaggccgg cctcgatgca ggcgtcgagg tgggcctcga cgatgtcgcg
     1441 gccgtacacc tcgtcggcgc cgacgccgca gtagtacggg ccctgggggc cggggaaacc
     1501 gccgcggggc cagccgagcg ggtgcccgtc ctcgaggaac gtgtactcct gctccatgcc
     1561 gaaccacatg tcctggtcgg cgtacttctc ggcgacctcg gcggtggccc gacgggtgtt
     1621 ggagacgtgg ggcttcatgt cgatgtcgag cacctcgttg agcacgagga tgctgtcgcc
     1681 gccacggatc gggtcggggc agacgaacac ggggttgagc acgcagtccg aggagtggcc
     1741 ctcggcctgg ttggtcgacg aaccgtcgaa gccccacacc ggcggctcct ggccctcggc
     1801 gaggatcttg gtcttggagc ggagcagggg cgtcggcttg gtgccgtcga tccagatgta
     1861 ttcggccttg atgctcacgg gtgtggttgc cttccgggaa gagtcggggg tgcggggttg
     1921 cagcgccatt caacgcgcga cgcgtgttcc gggcttttcg cgagtgtgaa cgctttgtga
     1981 accgggcgcc aggggccggt tccgcccgca tgcgaacggc ggtgggctgc gtaccatccg
     2041 ggccatggag cggcaacgtg agtacgtcct gcggaccgtc gaggagcgag gggtccggct
     2101 catccggctc tggttcaccg acgtgctcgg ccagctgaag tccttcgcca tctcgccggc
     2161 ggagctcgag gcggccttca gcgaggggat gcacttcgac ggctcggcca tcgacgggtt
     2221 cagtcgggtc caggagtccg acgtcctcgc cctccccgat cccaagacct tcgagatcct
     2281 gccgtggggt ccctcgcccg aggacaagaa ctccacggcg gcgatgttct gcgacatctc
     2341 caacctcgac ggcaccccct tcgccggcga cccccgccac gtgctcaagc gcaacctggc
     2401 cgcggccagc gagaagggct tcaccttctt cgtggccccc gagatggagt tcttctactt
     2461 cgagtcatcg ggcacccagg gccggccccc cgtgccgctc gacgacgggt cgtacttcga
     2521 cctcaccacc accgacgtgg ccaacgacct gcgccgcaag acgatccaca tgctcgaggc
     2581 cctcggcatc ccggtcgagt actcgttcca cgaggacagc ccgagccagc acgagatcga
     2641 cctgcgctac accgatgcgc tgtccatggc cgacaacgt
//
LOCUS       DATISU010000156         3132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_193779, whole genome shotgun sequence.
ACCESSION   DATISU010000156 DATISU010000000
VERSION     DATISU010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193779"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VLR27_06355"
     CDS             <1..1095
                     /locus_tag="VLR27_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP03103.1"
                     /translation="MTEKQGGSDVRANTTTATPADDGSYRLTGHKWFCSAPMSDVFLV
                     LAQSPGGLTCFLVPRWRPDGTRNPVRIQRLKDKLGDRSNASSEIELLDTFGERIGDEG
                     RGVRTIIEMVNHTRLDCVLGATAIHRQGVAQATWHAHHRAAFGATLDRQPLMANVLAD
                     LAIESEAATLSAIRLARAFDAPADDEHEQHMKRLLTPIVKYWTCKRAPMHTAEALECL
                     GGAGYVEESDLPRLFRQSPLNGVWEGSGNVICLDVLRAVGRAPETLDAYWTEVDAAAG
                     SDRRFDTAADALRKELGDFDDIELRARRLVEQMALLFQASQVLQHSPAPVADAFVASR
                     VGDDTRLAFGTLPTGLALDEVIDRARPVPT"
     gene            1118..1558
                     /locus_tag="VLR27_06360"
     CDS             1118..1558
                     /locus_tag="VLR27_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03104.1"
                     /translation="MPSDGLRTAGRWLLGGALLYAGAAHLTFARDEFRAQVPEWLPID
                     ADLVVLASGVAELGLGAALLAWRARRVEVGLATAAFFVAVFPGNIAQFVEGTDAFGLD
                     SDRARLVRLFFQPVLVVWALWSTGAWRAIRAGALGRGTSPSGHG"
     gene            complement(1509..2603)
                     /locus_tag="VLR27_06365"
     CDS             complement(1509..2603)
                     /locus_tag="VLR27_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HSP03105.1"
                     /translation="MKRITALSVAMVAAGFVLAPTAAHADDRVCTSSIGAETVDNVQV
                     PDGASCALTGTVVEGNVTVGTGATLTATDVDVDGNIQAEGHGDVTVTGSSRVGGSVQI
                     EQGASADVRGTTITGDLQYFSNTGTLSASDNTIGGNLQATQNSGGLDIRGNTIDGNLQ
                     CSANDPAPTGGDNVVEGSAEDQCAALEGGTTPPPPAGTFCDTDDHFHEANIDKVAAAG
                     ITQGDDDGCFRPDAPVTRGQMAAFIDRAYDLAPGAATFCDTAGTTHEASIAAVAEAGI
                     AEGTTDGCYRPGASVTRGQMASFIARAESLAPGGTSNFCDIAGTTHEDNIVAIADAGI
                     AQGDSAGCFNPGDDVTRGQMATFLARALQL"
     gene            complement(2704..>3132)
                     /locus_tag="VLR27_06370"
     CDS             complement(2704..>3132)
                     /locus_tag="VLR27_06370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HSP03106.1"
                     /translation="LEREGFDASGGGFPGMTVESAHQTMWSKGSPDILVIALGTNDMG
                     NGNVPVDAVRSTLSQWLQEVPDTCVVLVGVNEATEAWGLHEHAPPYNEMLRQLADEHG
                     DGHVVDWAPEPGMIGDDGIHLTEQGMAAYRELIVDGVERC"
BASE COUNT          398 a         1119 c         1129 g          486 t
ORIGIN      
        1 atgaccgaga agcagggcgg ctccgacgtc cgggccaaca ccaccacggc gacgccggcc
       61 gacgacggct cgtaccggct caccggccac aagtggttct gctccgcgcc gatgtccgac
      121 gtgttcctcg tgctcgccca atcgcccggc gggctcacct gcttcctcgt gccgcgctgg
      181 cgaccagacg gcacccgcaa cccggtccgc atccagcggc tgaaggacaa gctcggcgat
      241 cggtcgaacg catcgagcga gatcgagctg ctcgacacgt tcggcgagcg catcggcgac
      301 gagggccgcg gggtccgcac gatcatcgag atggtgaacc acacccggct cgactgcgtg
      361 ctcggcgcca ccgccatcca ccgccagggc gtggcccagg ccacatggca cgcccaccac
      421 cgcgccgcct tcggcgccac cctcgaccgc cagccgctga tggccaacgt gctcgccgac
      481 ctcgccatcg agtccgaggc ggccacactc tcggccatcc gcctcgcccg cgccttcgac
      541 gcgccggccg acgacgagca cgagcagcac atgaagcggc tgctcacccc gatcgtgaag
      601 tactggacgt gcaagcgggc accgatgcac acggccgagg cgctggagtg cctgggtggc
      661 gccggctacg tcgaggagtc cgacctgccc cgcctgttcc gccagtcgcc gctgaacggc
      721 gtgtgggagg gctcgggcaa cgtcatctgc ctcgacgtgc tccgagccgt ggggcgtgcg
      781 cccgaaaccc tcgacgccta ctggaccgag gtcgacgctg ccgccggcag cgatcgccgc
      841 ttcgacaccg ccgccgacgc cctccgcaag gagctgggcg acttcgacga catcgagctg
      901 cgggcccggc gactcgtcga gcagatggcg ctgctgttcc aggcgtcgca ggtgctgcag
      961 cactcgcccg ccccggtggc cgacgcgttc gttgcgtcac gggtgggcga cgacacccgc
     1021 ctggcgttcg ggacgctgcc caccggcctg gcgctggacg aggtcatcga ccgggcccgc
     1081 cctgtcccga catgatcgac gcagcagcct gagcccggtg ccctccgacg gactccgcac
     1141 cgccgggagg tggttgctcg gcggcgcgct cctctacgcg ggtgcggccc acctgacctt
     1201 cgcccgggac gagttccggg cccaggtccc ggagtggctc ccgatcgatg ccgacctggt
     1261 ggtgctggcc tcgggcgtcg ccgagctcgg gctcggcgcc gccctgctgg cctggcgggc
     1321 acgccgggtg gaggtgggcc tcgccaccgc agcgttcttc gtcgccgtct tccccggcaa
     1381 catcgcccag ttcgtcgagg gcaccgacgc cttcgggctc gactccgacc gagcccggct
     1441 ggtccgtctc ttcttccagc cggtgctcgt ggtctgggcg ctgtggtcga ccggggcgtg
     1501 gcgggcgatc agagctggag cgctcgggcg aggaacgtcg ccatctggcc acgggtgacg
     1561 tcgtccccgg ggttgaagca gccggcgctg tcgccctggg cgatgccggc gtcggcgatg
     1621 gccacgatgt tgtcctcgtg ggtggtcccg gcgatgtcgc agaagttgct ggtgccgccg
     1681 ggggcaaggc tctcggcccg ggcgatgaag ctcgccatct gaccgcgggt cacggacgca
     1741 cccggccggt agcagccatc ggtggtgccc tcggcgatcc cggcctcggc cacggcggcg
     1801 atgctggcct cgtgcgtggt gcccgccgtg tcgcagaacg tcgctgcgcc gggggcgagg
     1861 tcgtaggccc ggtcgatgaa ggcggccatc tgaccgcggg tcaccggagc gtccggacgg
     1921 aagcagccgt cgtcgtcgcc ctgggtgatc cccgctgccg cgaccttgtc gatgttggcc
     1981 tcgtggaagt ggtcgtcggt gtcgcagaag gtgccggccg gcgggggcgg ggtcgtaccg
     2041 ccctcgaggg cggcgcactg gtcctcagcg gagccctcga cgacgttgtc cccgcccgtg
     2101 ggcgccgggt cgttggcgct gcactgcagg ttcccgtcga tggtgttgcc gcggatgtcg
     2161 aggccgccgg agttctgggt ggcctggagg ttgccgccga tggtgttgtc cgacgccgac
     2221 agcgtcccgg tgttggagaa gtactgcaga tcaccggtga tggtggtgcc gcgcacgtcc
     2281 gcgctggcgc cctgctcgat ctggacgctg ccgccgacgc gtgagctgcc ggtgaccgtg
     2341 acgtcgccgt gcccctcggc ctggatgttg ccgtcgacgt ccacgtcggt cgccgtcagg
     2401 gtggcgcccg tcccgaccgt gacgttcccc tcgaccacgg tgccggtcaa ggcgcagctg
     2461 gcgccgtcgg ggacctggac gttgtcgacg gtctcggcgc cgatgctcga cgtgcagacc
     2521 cgatcgtcgg cgtgggcagc ggtgggggcg agcacgaagc cggcagcgac catggcgacg
     2581 gacagtgcgg tgatgcgctt catggggacc tctgacgtgg tggtgcggcg gactgtccgc
     2641 tcaggatcgg ctgcggggat gaagggagcg tgaggcgcag atgagagcgc gctcatctgg
     2701 ccctcagcag cgctcgacgc cgtcgacgat cagctcccgg tacgcggcca tgccctgctc
     2761 ggtcaggtgg atgccgtcgt cgccgatcat ccccggttcg ggcgcccagt cgacgacgtg
     2821 gccgtcgccg tgctcgtcgg cgagctggcg cagcatctcg ttgtagggag gggcgtgctc
     2881 gtggagtccc cacgcctccg tggcctcgtt cacgcccacc aggacgacgc aggtgtccgg
     2941 gacctcctgc agccactgcg acagggtcga gcggaccgcg tcgacgggca cgttcccgtt
     3001 gcccatgtcg ttggtgccca gcgcgatcac gaggatgtcc ggagacccct tcgaccacat
     3061 cgtctggtgc gcggactcga ccgtcatgcc ggggaagccc ccgccgctcg cgtcgaagcc
     3121 ctcgcgctcc ag
//
LOCUS       DATISU010000157         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_752299, whole genome shotgun sequence.
ACCESSION   DATISU010000157 DATISU010000000
VERSION     DATISU010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752299"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..611
                     /locus_tag="VLR27_06375"
     CDS             117..611
                     /locus_tag="VLR27_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSP03107.1"
                     /translation="MADDQDLDLELELDVPSRGTAPRVAVVAEDEPGIRSLIVSLLTL
                     DGWEVHVTDTGENVADLIDQYHPVLCLLDVMMPGMDGVEAMEEGRARGHGAPDGTVYV
                     ILTALAGQEDVIRGVVSGADDYITKPFDVDDLSDRVARWCEATGWELVDAEHPGLVHE
                     HLRY"
     gene            618..1382
                     /locus_tag="VLR27_06380"
     CDS             618..1382
                     /locus_tag="VLR27_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP03108.1"
                     /translation="MGDDVPELVGSKRRGRAAVRELEDAHLVAAFQQSRDKDAVNELL
                     RRHEGLIMSVARRYFVPGGDHEDVRQQAMIGFWKAVRDFTPDAGASFPSFSRTCMQRQ
                     VITAVKAANRMKHSPLNESDRIAHHDAEHDEDHGSSAVQLPSVSHEGSAVERMDMDDS
                     GGMPFGLSEIESLLEAHGHERVHEILRLHFEGKSTRRKKDEPDRLSENEAHVLVGLLS
                     GKKYREIAEQMGKTDKFVDNTVQRLRAKLRHLLEDG"
     gene            complement(1379..1840)
                     /locus_tag="VLR27_06385"
     CDS             complement(1379..1840)
                     /locus_tag="VLR27_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03109.1"
                     /translation="MTRSTRAASAIGAAVLALVLVVPEQAGAQLLPTTTEPPATTEPV
                     PETTVPPETTAPPTTAPPTTAPPTTAPPATRAPTTAAPTTEPPTTTTTAPPSAPSTAP
                     TTTVPVEGRARITQDTPALGGVLTLWGLGIVGTLATLAWSWQRNRTSGGGA"
     gene            complement(1848..2552)
                     /locus_tag="VLR27_06390"
     CDS             complement(1848..2552)
                     /locus_tag="VLR27_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03110.1"
                     /translation="MLHADEMATIWVVRAGPKDRLVRSFLDDGEIGVAFPQFPDGHDV
                     DRAKALKILGVGLDDEPSKHQEADAALFLSFVRRIRVADVALLLDPVAGGFACGVVTG
                     EYHYRGDLDGDRAQHRRPVDWRRRLPFTALPARLDHLPDQRSMLEDVPDGRLRDLALQ
                     CCRGELGEDPFDRPAATPPRRRATSSGGSGRPRASRPPAPKRPSQVTRAERRCTVCLV
                     TKSEDLFDGDVCRDCA"
     gene            2578..2859
                     /locus_tag="VLR27_06395"
     CDS             2578..2859
                     /locus_tag="VLR27_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HSP03111.1"
                     /translation="MHVWIDQDLCTGDGLCTDHCPEIFVILEDGIAYVRDGMDIGNDP
                     GGAGSMVPVAPDFQQRVVDSAELCPGECIFIEIDETDLGELEELDRAGA"
     gene            complement(2861..>3041)
                     /locus_tag="VLR27_06400"
     CDS             complement(2861..>3041)
                     /locus_tag="VLR27_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HSP03112.1"
                     /translation="DRDACQGHNRCYQLAPELFDVDEEGYAVLLVTGEVPAELEEKAK
                     LCADNCPEFAIEVEG"
BASE COUNT          426 a         1047 c         1149 g          419 t
ORIGIN      
        1 gcccatgggt cgcctcgcct gtcggctcgc ctcctgcgcg tccgaccgcg tcgcgacctc
       61 gggtcggagg taccgatggt cctcacgtgg ctgctagcta gcccacgtcg acgcgcatgg
      121 cagacgacca ggacctcgac ctcgaactcg aactcgacgt gccctcccgg ggcacggcgc
      181 cccgtgtcgc agtggtggcc gaggacgagc ccgggatccg ctcgctcatc gtctcgctgc
      241 tgacgctcga cggctgggag gtccacgtga ccgacaccgg cgagaacgtg gccgacctga
      301 tcgaccagta ccaccccgtg ctgtgcctgc tcgacgtgat gatgccgggc atggacggcg
      361 tggaggccat ggaggagggg cgggcccggg gccacggcgc ccccgacggc accgtctacg
      421 tgatcctcac cgcgctcgcc ggccaggagg acgtcatccg aggggtcgtg tccggcgccg
      481 acgactacat caccaagccc ttcgacgtcg acgacctgtc cgaccgggtg gcgcgctggt
      541 gcgaggcgac gggctgggag ctcgtcgacg ccgagcaccc gggcctcgtg cacgagcacc
      601 tgaggtactg accggtcgtg ggggacgacg tgccggagct ggtcgggtcg aagcggcggg
      661 gccgcgctgc cgtgcgtgag ctcgaggacg cgcacctcgt cgccgcgttc cagcagagcc
      721 gggacaagga cgcggtcaac gagctgctcc gtcgccacga ggggctcatc atgtccgtcg
      781 cccggcggta cttcgtgccg ggcggcgacc acgaggacgt gcgccagcag gcgatgatcg
      841 gcttctggaa ggccgtccgt gacttcacgc ccgacgccgg ggcgtcgttc cccagcttct
      901 cccgcacctg catgcagcgc caggtcatca ccgccgtcaa ggccgccaac cgcatgaagc
      961 actcgccgct gaacgagtcc gatcgcatcg cccaccacga cgccgagcac gacgaggacc
     1021 acggatcctc ggccgtgcag ctgccgtcgg tgagccacga gggttcggcc gtcgagcgca
     1081 tggacatgga cgacagcggc ggcatgccct tcggcctgtc ggagatcgag agcctgctcg
     1141 aggcccacgg ccacgagcgg gtgcacgaga tcctccggct ccacttcgag ggcaagagca
     1201 cgcgtcggaa gaaggacgag cccgaccgcc tgtcggagaa cgaggcccac gtgctcgtcg
     1261 gcctgctcag cggcaagaag taccgcgaga tcgccgagca gatgggcaag accgacaagt
     1321 tcgtggacaa cacggtccag cggctgcgag cgaagctccg ccacctcctc gaggacggtt
     1381 aggcccctcc gcccgacgtc cggttgcgct gccacgacca cgccagggtg gccagcgtgc
     1441 cgacgatgcc gaggccccac agcgtgagca cgccgccgag ggcgggcgtg tcctgggtga
     1501 tgcgggcgcg accctcgacc ggcaccgtcg tggtgggggc cgtcgacggc gcgctcggcg
     1561 gcgccgtggt ggttgtcgtg ggcggctcgg tggtgggcgc ggccgtggtg ggtgcccggg
     1621 tcgcgggcgg tgccgtcgtc ggcggcgcgg tggtcggcgg cgcggtggtc ggcggtgccg
     1681 tggtctcggg cggcaccgtg gtctccggca cgggctcggt ggtggccggt ggctccgtgg
     1741 tggtgggcag cagctgcgca cccgcctgct ccgggacgac cagcaccagg gcgaggacgg
     1801 ccgcgccgat cgccgacgcg gctcgggtcg atcgggtcac cggacggtca ggcgcagtcg
     1861 cggcagacgt caccgtcgaa gaggtcctcg gacttggtga ccaggcacac ggtgcagcgg
     1921 cgctcggcgc gcgtgacctg cgacggccgc ttcggcgccg gcgggcgcga cgcgcgtggt
     1981 cggccgctgc cacccgagct cgtggcccgg cgtcggggcg gggtggcggc cggccggtcg
     2041 aaggggtcct cgccgagctc gccccggcag cactgcaggg ccaggtcgcg caggcggccg
     2101 tcgggcacgt cctcgagcat ggagcgctgg tcgggcaggt ggtcgaggcg ggcgggcagc
     2161 gcggtgaagg ggaggcgccg gcgccagtcg accggacggc ggtgctgggc ccggtcgccg
     2221 tcgaggtcgc cccggtagtg gtactcgccg gtgaccacgc cgcaggcgaa gccgcccgcc
     2281 accgggtcga gcagcagcgc gacgtcggcc acccggatgc ggcgcacgaa gctcaggaac
     2341 agcgcggcat cggcctcctg gtgcttgctc ggctcgtcgt cgaggccgac gccgaggatc
     2401 ttgagcgcct tggcccggtc gacgtcgtgg ccgtcgggga actgggggaa ggccaccccg
     2461 atctccccgt cgtccaggaa cgagcgcacg aggcgatcct tgggaccggc ccgcaccacc
     2521 cagatcgtgg ccatctcgtc ggcgtgcaac atcaggatcc gaccctacgc tggccgggtg
     2581 cacgtgtgga tcgaccagga cctctgtacc ggcgacggtc tgtgcaccga ccactgcccg
     2641 gagatcttcg tcatcctcga ggacggcatc gcctacgtgc gcgacggcat ggatatcggc
     2701 aacgaccccg ggggcgccgg ctccatggtg ccggtggcgc ccgacttcca gcagcgggtg
     2761 gtcgactcgg ccgagctgtg cccgggcgag tgcatcttca tcgagatcga tgaaacggac
     2821 ctcggtgagc tggaagagct cgaccgggct ggggcttgag ctacccctcg acctcgatgg
     2881 cgaactcggg gcagttgtcc gcgcagagct tggccttctc ctcgagctcg gcggggacct
     2941 cgccggtgac gagcagcacg gcgtagccct cctcgtcgac gtcgaagagc tcgggggcga
     3001 gctggtagca gcggttgtgg ccctggcagg cgtcgcggtc g
//
LOCUS       DATISU010000158        24573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_333236, whole genome shotgun sequence.
ACCESSION   DATISU010000158 DATISU010000000
VERSION     DATISU010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 24573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24573
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333236"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2148)
                     /locus_tag="VLR27_06405"
     CDS             complement(<1..2148)
                     /locus_tag="VLR27_06405"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HSP03113.1"
                     /translation="MRTRVDLPVEGMTCAACSARISRGLNKLDGVENANVSLASARAT
                     VIFDDEQLAADDFEAVITKLGYSVPEEADHEAAEAEWIHTITRRLLIAAVLSVPVVLL
                     SMIPALQFDGWAWVVFALTTPVVFVSGIGFHRAALLNARHGAATMDTLVSIGTLAAYL
                     WSAVALIFLGAGDSTMDMAGMGDPATGDAHVYFETAAVIITLILLGKWFEARAKRRSG
                     EAIRALADLGARTATLEDGTEIAVDDLAVGMRFIVKPGEKVPTDGRIVDGASALDESM
                     LTGEPVPVDKSVGDEVIGATVSTDGRLVVEATRVGADTALAQIVRMVEEAQGSQAPVQ
                     RLADRISAVFVPIVLLIATATLTGWFLTGHGADDAFTAAVAVLIIACPCALGLATPTA
                     IMVGTGRGAQMGVLIKGGEILERTRSVDTVVFDKTGTLTEARMQLVSAESFDPDVEDA
                     VLLRRAASLEAMSEHPIAAAIAAGTTDHRPVEGFRNLAGKGVHGTVDGVEVLVGRRML
                     FDEVPAEVEAAAAAQEAEGRTAVLAGWDGRARGVLAVSDTVKPSAVEAVAALREMGLE
                     TVLLTGDNRRTAEAVAAQVGISTVLAEVLPDDKLAEVRRLQDAGRVVAMVGDGVNDAP
                     ALAASDLGIAIGTGTDVAIEASDLTLVSGDPRGVADAIALSRRTLSTIKGNLFWAFAY
                     NTAAIPLAAFGLLVPMIAAGAMGFSSVFVVTNSL"
     gene            2207..2500
                     /locus_tag="VLR27_06410"
     CDS             2207..2500
                     /locus_tag="VLR27_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HSP03114.1"
                     /translation="MPGYTMNKDQYQARLRRIEGQVRGLQRMVDEDTYCIDVLTQIAS
                     VTKALQGVGMGLLDEHIRHCVREAAELDGPDAEQAFDDKVHEVTAAVSRMLRT"
     gene            2528..3382
                     /locus_tag="VLR27_06415"
     CDS             2528..3382
                     /locus_tag="VLR27_06415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HSP03115.1"
                     /translation="MHLGVMMFPTDKAIDPVTLAREVEARGFESLWFPEHSHIPTSRV
                     TPWGGREGAPPLPEEYWRTHDSLISLGACAAVTSTLKLGTGICLVAQRDPIWLAKEVA
                     TVDRISSGRFLFGIGYGWNKEELAHHGVRYGDRRDVLRERVLACKQLWTQDEASFDGE
                     HVHLEPSWSWPKPVQQPHPPVILGGAAGPKTFAHIAEFCDGWMPIAGRHALVDQLDDL
                     RAACEAVDRDPATVELGQFATPARPEALEELASAGVSRAVFGLPPGDADTVLPLLDRY
                     AELIDATP"
     gene            3455..5407
                     /locus_tag="VLR27_06420"
     CDS             3455..5407
                     /locus_tag="VLR27_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HSP03116.1"
                     /translation="MSDVQRRERDRPWMMRTYSGHSTAKASNELYRTNLARGQTGLSI
                     AFDLPTQTGYDPDHVLARGEVGKVGVPVAHLGHMDTLLDGIPQDQMNTSMTINAPAAW
                     MLGLYVANAEKKGIESKALRGTTQNDIVKEYLSRGTYIFPPVPSRRLIVDMIAFCAEW
                     IPKWNPMNVCSYHLQEAGATPVQEIAYSLANAIGVLDAVKESGQVDDDRFAQVFGSIS
                     FFVNAGIRFVEETAKMRAFTQLWERIGRERYGVTDDKALRFRYGVQVNSLGLTEQQAE
                     NNVQRIVLEALGVTLSKSARARSIQLPAWNEALGLPRPWDQQWSLRVQQVLAHETDLL
                     EYEDIFDGSHVMEGLTAELVEASWAEMTDILDLGGAFEAIDEMKGRLVRSHAERIRRI
                     ESGDQVVVGVNAYTETAPSPLGGEDSIMKVDPGVQDMLIADVQQWRSERDSDAVKRSL
                     DELRRVAESGENIMPATIDLAHAGGTTGEWSGLLREVFGEYRAPTGVAAAAGHGTSQG
                     LRDVAEKIKARPSGPPRLLVAKPGLDGHSNGAEQIAVAARDAGFEVIYQGIRLTPAQI
                     AQVAADEDVDAIGLSILSGSHLELVPETVRLIREAGSDAPVIAGGIIPEDDRSVLTDA
                     GVAAVYTPKDFDLTKIMGEILDLVSD"
     gene            5418..6149
                     /locus_tag="VLR27_06425"
     CDS             5418..6149
                     /locus_tag="VLR27_06425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSP03117.1"
                     /translation="MGVSRAGGRWTVVAAAVVAGVAGVLAVLTAAAVPAVVAGIASAL
                     VAVLVLIDDPEEHEPAPPPAPPADPVPEAPEADEDIDPIRAIFESGPPAGGSGMLSPE
                     FLLTTLRGRVAVARRALRPLSIVCFEAYETDADGATTSPVAAEDVGAVLRRTLREADV
                     AGHLHGDAFVCILEDTGENGAVWTAERVRRNLLEGGQARRFRAGVASYPAHGLEPEEL
                     EAKARAALVAARDWATDRIEVAGAP"
     gene            complement(6150..7271)
                     /locus_tag="VLR27_06430"
     CDS             complement(6150..7271)
                     /locus_tag="VLR27_06430"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSP03118.1"
                     /translation="MTAQLPLADLRVIDASTVLAGPACARYLADFGADVIKVERPGSG
                     DGTRDMFRRDPADDVTYFWKLLGRGKRTIVLDLKSDEGRGHMLDLCASADVLVENFRP
                     GTLERLGLGPDVIHERNPDLVITRVTGFGQDGPYAGRAGFATLAEAMSGFASLSGEPD
                     GAPLLPPIALTDEVTGLTAAFATMVAVHSGVGQVVDVNLLESLLQIMGPLVSASAVEG
                     WEQPRLGSGVPYTVPRGTWQCADGGWVAVSTSAESVARRVLDLVGLGDDERLQTFDGR
                     VAAREQIDARMAEWIGERTRDDVLAAFEEAHAACAPVYSMNEVLADPHVRARGAVEEV
                     DGTPMQGLVARLSATPGRIRWTGRPLGADTDEVLDELSD"
     gene            complement(7268..8278)
                     /locus_tag="VLR27_06435"
     CDS             complement(7268..8278)
                     /locus_tag="VLR27_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSP03119.1"
                     /translation="MGPEITTVGPDLVVVHRGTDVRRYDDLEAGRVHDLDGLEIETMP
                     HPGGELLARFATVNDVHFGETRCGVVEGHEDEIGPILTAEPGEPPYPVTMNEAAIAEI
                     QAAEVDAVFVKGDLTNDGTDEEYAAFLDTYGGAFGDRLTHVRGNHDAYEGQSYADWPL
                     QRVDLPGATVVLLDTSVPFKSSGGLTHDQLDELDAIAADADRPVVVLGHHHVWDPRSK
                     ERNETYFGIHPDWSERLIEVAARRPSIVGYLAGHTHRNRVRRFPQTGAMPWVEVACVK
                     DYPGAWAEYRVHEGGIVQLHHRISSPEALDWTERTRHMYLGMYADYAFGRLADRCFML
                     ER"
     gene            complement(8287..8460)
                     /locus_tag="VLR27_06440"
     CDS             complement(8287..8460)
                     /locus_tag="VLR27_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03120.1"
                     /translation="MQIQPAPTDEEAVAIAAAVEVAWPRVGGETPDDRPNRWRFSGRW
                     WSQPVPMRRNRPW"
     gene            complement(8471..9484)
                     /locus_tag="VLR27_06445"
     CDS             complement(8471..9484)
                     /locus_tag="VLR27_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP03121.1"
                     /translation="MASVAFAAVTMERAGRTALRSLTLDVDDGTICCLVGPSGCGKST
                     AVRLLAGLEAPTSGEIRIGDRRVNEVPTRDRGLGMVTQASALLPSRPTEDSVGLPLDL
                     RRDIDRDERRFRIDEQALALDLGGVLGRPPSTLSGGEIQVAQIARAVIARPRVLLLDE
                     PLARIDPARRGMVRADLVRLQQLYGVTTIWVTADQRDALAVADRVAVLIDGRLEQAGP
                     PMHVYDRPRTLAVARFFGEPEIGVVRTELDGREVDVGIRPHELRLATPGTTGIRFPAV
                     VVGVELVGRRAIATVTVDGGVLRWDDAPTSTRVGDRIELVVDPSRVHLFDPGTGVAVG
                     HPE"
     gene            complement(9484..10611)
                     /locus_tag="VLR27_06450"
     CDS             complement(9484..10611)
                     /locus_tag="VLR27_06450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP03122.1"
                     /translation="MQVPIRLLRAIRPSEHPGLLLATAYGTAVLSVAPFLLPALSDEY
                     GIGLGLASFATAAQLLGFVAGSWGAGRFLRPRGRVLGLALLVSVAAHGLSALLPPFGV
                     LVGLRVVSGVALGLITWFSWTLVFGDDQRMSEIAVVGPIIGITTAPIAAALVDRFGPR
                     GIFVALAVTALVPLAFSRHSGSDHERIVTPRAERAAGSRSRPAPGVRPILLLLGLMTV
                     GGSSVFTFGAVIAADRTTLSLSAISLAYSANALASIPSARWSGRRGPAGAWIVGCGVC
                     AVVMGAATSGPLFVVAIAFWGFAFWMGTPGAFSLLAARSANPGERAGDAQAVMAAGRV
                     VGPLAGGVLLDATSAGVLGIVGGVVVGAAGAGLVLVERRDA"
     gene            complement(10658..12211)
                     /locus_tag="VLR27_06455"
     CDS             complement(10658..12211)
                     /locus_tag="VLR27_06455"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HSP03123.1"
                     /translation="MAEHPMSERIADLEEKKAEARQPGSERSIARQHERGKMLARERI
                     EYLLDEDSFHELDLLARRPMESSFDERPYSDGVITGWGTVDGKKVFVFSQDFTVYGGA
                     LGEVFANKIHKLMDMALKVGAPLIGLNDGAGARIQEGVVSLDSYGGIFYRNVLASGVT
                     PQISVIMGPCAGGAVYSPAMTDFVFMVEDTSYMFITGPDVVKTVTGADVTQQELGGAD
                     AHSQKSGVCQFTGPDDRAVLDDVKYLLSFLPSNNLEVPPVLNTGDDPERQCDALRDMV
                     PTNPNQPYDMKAVIAEIVDDGDYFEYAPAWAPQITCGFARMAGRPVGIVGNQPMAMAG
                     VLDIESSEKAARFVRTCDAFNIPLLTFVDVPGFMPGVDQEHGGIIRHGAKLLYAYCES
                     TVPRIQIITRKAYGGAYVVMNSRSIGADYAIAWPSAELAVMGAAGAVEIVNRREILDS
                     DDPDAKKAELVEEYSEKFLNPYAAADRGYVDDVIDPADTRRKVNAALDMLESKREELP
                     ARKHGNVPL"
     gene            12331..14682
                     /locus_tag="VLR27_06460"
     CDS             12331..14682
                     /locus_tag="VLR27_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSP03124.1"
                     /translation="MRKSPDVSRRRFLGYVVAGPTLITAARWIDPLATADAQELPSIG
                     PQIAESYDLIDFLRDAARPTANLITVTVEDDGTVSFELHRTEVGQGINTAIAMIIAEE
                     MDVPLSQVRITQADARPELLFNQLTGGSNSIYTLYDPVRIAAAVARQRLLDAAAIALG
                     SAGAELGILDGVIRAPDGSSIGFGELARDAASQVTEAVDVQPKRERPRLVGTPQNRID
                     ARAMVTGELRYTGDLVVPDALPTMVARPPTHLGRPVRLRNEAAVLAVPGVTHVALVES
                     GVAVRARTFGQCIDALQLIDVEWEPGPAAGMSDESVQAALADAELPQAVPDTGAIGAL
                     GIPSPVQTRDFTFTFAFQPNCPLETNPCVADVRADRAEIWGPMKNPVVAQQDIADALG
                     LPQDAVTVHVVQGGGSFGRDLFWDAAREAALVSQAMGVPVKLSWTRTDDFRQGRAHPA
                     CRSSIRITRSDTDIVSYAQHHTSVNTDFGHGLGEIISARVDELPGGLGGMGFSQTVFM
                     LTQYVPYDFGAVEQLLTETLPSPGEIETNVFRTGSMRNIYSPNVVTARELAVDQVAAE
                     VGRDPLELRLTTMRDARSRAVLEKVAEAGGWGREMTPGTAQGLGFHSEYKGRAACLVE
                     IDCRPETVSRTVPTAAYAYTGPRVTRAIYAVDVGLTINPRGLEAQMLGGMMDGIANCL
                     SFSLHLVDGAFAEDSWEHAYYTRQWNVPFETQVFILDSSSQPGGAGEFGVAASMAATA
                     NAYARATGSMPTSFPINHGMPLAESIVPQGTIPQSPTDGLGRR"
     gene            14694..15212
                     /locus_tag="VLR27_06465"
     CDS             14694..15212
                     /locus_tag="VLR27_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSP03125.1"
                     /translation="MPVQKFTVNGQRVEVDVEDDVRLLWVLRDLLDLTGPKYGCGLSI
                     CQSCTSLVNGKAFNPCSVPVGQLRPSDEVITIEGLADAVDGDGLHPVQEVWLEEDVAQ
                     CGFCQPGQIMAAVDLIQTLLASGRSDVTEAELDSLRNVCRCGTYPRVRTAIRRALTVM
                     AETPVFVRGAGR"
     gene            15236..15649
                     /locus_tag="VLR27_06470"
     CDS             15236..15649
                     /locus_tag="VLR27_06470"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HSP03126.1"
                     /translation="MHRLFVYGTLQHPPLLEHLLGRSPALEAATVAGWRAARLRGRVY
                     PGLVPAPGSSAHGHVIEVDDDELVVLDRFEGPQYERIVVSADGHEVGAWRLRSEHVPL
                     ALEEDWDLDRFVAEDAVAFLGASRPGDEHPWDGQR"
     gene            complement(15640..17106)
                     /locus_tag="VLR27_06475"
     CDS             complement(15640..17106)
                     /locus_tag="VLR27_06475"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSP03127.1"
                     /translation="MPNDHVDVLIVGAGISGIGAAHHLLADRPGTTFTILEGQEGYGG
                     TWRTHTYPGVRSDSDLYTFGYSFKPWTGAPIATAQEILTYLGEVIEDDGLDEHIRYQH
                     QIDTAAWSTEDRRWIVTGTRTDTGEAFTVSAGFLWMCQGYYRHTEGYTPEWDGTDDFT
                     GQIVHPQTWPDDLDYADKRVVVIGSGATAATLIPAMAPDCEHITMLQRSPTYFATGRN
                     ANELADQLRDLEVPDEWTHEIVRRQVLKTQALVTSMSAEHPELVKDELFKGIKAILGD
                     DYDLSPHFTPAYRPWQQRIAFVPNGDLFEGIKSGKASVVTDTIDRFVPEGIRLESGEV
                     LEADLIITATGFEMSVLGDIAFTVDDEPLDFAQTVGYRGTMFTGVPNMAWVFGYFRAS
                     WTLRADLIADFVCRLLDHMDELGADIVVPQLREEDADMGLLPWVDPEDFNPGYLMRAL
                     HLMPKQGTKEPWRFTQDYWAEKDEFPAADLDDGALLYR"
     gene            17190..18284
                     /locus_tag="VLR27_06480"
     CDS             17190..18284
                     /locus_tag="VLR27_06480"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HSP03128.1"
                     /translation="MDLPVPFPLKPMLAKANDGIPTGDWVFEPKWDGFRCLVFRDGDE
                     IQLGSRNERPLTRYFPELLDPLRAALPERCVVDGEIVVAIGDRLDFDALGQRIHPAES
                     RVNMLAEQTPAAFVAFDIVALDDRALLELPFAERRALLEQAITPGAQVHLTPVTVDPD
                     VAADWFRRVEGAGLDGLIAKDPASTYQPDKRILRKVKHQRTADCVAAGFRWHKDGKGV
                     GSILLGIHAGTPDDPGDLRHVGVSASFTAKRRAELVDELAPFVMDPGTDEFAEHPWSS
                     WYDAEAHETGQLPGAPNRWNSQRDQSWIPLRTERVVEVGYTIMTAGRFRGTTRFVRWR
                     PDKEPHQCTYDQLDVVPPMAFADVLSPPDA"
     gene            complement(18291..19154)
                     /locus_tag="VLR27_06485"
     CDS             complement(18291..19154)
                     /locus_tag="VLR27_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HSP03129.1"
                     /translation="MGLTVHLVDGTYELFRYHYSPNNRDPERGANRGVVNSMLQLVED
                     GATHIGVATDHVIESYRNDLWADYKTGEGIDPQLKAQFPVLEEVLAAAGFAVWPQTTF
                     EADDGLAAGAVLAAADDDVERVVICTPDKDMAQLVGGKVVQWDRRQDKWFDTAGVQEK
                     FGVLPESIPDYLALVGDSADGFPGLPGWGAKSAAAVLARYVHIEDIPPSAGQWDITVR
                     SGAKLAKVLQDQLDDALLFRTLATLQPDAPISATVDDMTWRGPRDGFADVAASLDGDR
                     LAERADRLARR"
     gene            complement(19292..20266)
                     /locus_tag="VLR27_06490"
     CDS             complement(19292..20266)
                     /locus_tag="VLR27_06490"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HSP03130.1"
                     /translation="MRDYLPVGLGCLLVFLVMADVFRTVLWSGQGAGPLTGTITGIGR
                     RLLPLLSGNRRARSAVGPVALLAVVVTWTAVLLIGFTLMLEGQPDAIRTSGTKQPVDW
                     FERAYFVGYSLFTLGNGDLAPVTDFGRLLAVAVSATGLFLLTLSVTYLLPVISASVAS
                     RSFASSARSLGDTPEDVVLGAWDGDRIQLDHQMRDLATALGTLAEQHLAYPVLHLFQS
                     SDTATSAPLAVADLDDLLTLLDGVDPRAAPLATPRRQLRSAIETYLETYGSAVDGDGT
                     PTTPRTDRLDAAGIPLLDRAAYERTLDDLTTHRKKVRSLVRAAGVPSR"
     gene            complement(20302..21198)
                     /locus_tag="VLR27_06495"
     CDS             complement(20302..21198)
                     /locus_tag="VLR27_06495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HSP03131.1"
                     /translation="MAAVRSDRLTVVRYDVFTDRAYAGNPLAVAIEPPALSDAQMQRI
                     AAELNLSETVFLTDLGGGAWRARIFTPATELPFAGHPTIGAALALQDADLLRDGRVTL
                     HEGIGPVEVSLADGLATLTTPGRPEPVDTADPADVVAAVGLGLADLHPELGPRGWSAG
                     VPYTMVGLRDVGALERAELDPAAWRHGVGCSDAPDLYLLAPVDGVRGERWRARMFGPV
                     IGVAEDPATGSAAAAACGYLAGVAGERRLDEGWTIEQGVEMGRPSEMRVQAVRNGNEL
                     VAVRVGGRAVRVGSGELLLPEP"
     gene            21314..23320
                     /locus_tag="VLR27_06500"
     CDS             21314..23320
                     /locus_tag="VLR27_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HSP03132.1"
                     /translation="MTDNERPQGSTVAAGPPTSAPSGELSGICRRVVLWYAAVAATWI
                     VVSDLTVSAVRNELAEQQAVNVVKGLAFVLVTTAVLWVVLRRLTRSYDQHFAREVTAQ
                     RDFFQSILNTSTDLVLILGAGGAIRYASHALIEVLGWDHDEVVGRRGRDFLHPDDVSS
                     ASSFRANLGLGGPNRRTFRMAHRDGTYRTLEMAMSTISLGDGTEGAVVLARDVTERAR
                     GERQLRAALAEDATGLPNLHMFVAEMERLAEVTTVGLDAVVLLVDIDRFGDINALHGR
                     VGGDAVLLELSRRLEATLPQAIGLWRHGADEILALLLEDPAGPDAVGEVGLARLVERV
                     QHEAAAPIAIDDTDRRLSVSVSVGVARLPIDSAVPEDSLSSRMLRTAEEALAEAKQHP
                     DRSAVRVQRDAAAAGSDRALVIEQLHDAVARGELVVHFQPKIRLSDLSVVGAEALVRW
                     EHPDRGLLPPGEFLRTVDEANLTAALTRSVLRDALAQVPGWLGAEGCNPDFEVCVNIS
                     LDDLRRRRFVDDVFDALHESGVEAQHLSLELTEQTMLADALGASTVVAELRRAGIRVA
                     IDDFGTGFSSLEHIRIFEVDELKIDKGFVQQIGRSRTDEAIVDSILAIAGRLGVKVTA
                     EGIEAPDALEYLRQRGCHEGQGFLFSAAVPVEEFAPGRSWATLA"
     gene            complement(23317..23724)
                     /gene="mce"
                     /locus_tag="VLR27_06505"
     CDS             complement(23317..23724)
                     /gene="mce"
                     /locus_tag="VLR27_06505"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HSP03133.1"
                     /translation="MLLTEIDHVAIAVKDLDAAIAYYAEAFGAEVDHREVVDSDGVEE
                     ALVKVADSYIQLTAATRDDSPIAKFLEKKGEGLHHIGYRVDDCAQALESMVAAGATPI
                     DKAPRPGSRGTTVAFIHPKGSLGTLIELVQENL"
     gene            23829..>24573
                     /locus_tag="VLR27_06510"
     CDS             23829..>24573
                     /locus_tag="VLR27_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03134.1"
                     /translation="MTTMTRAYAITAEAVAAETEANGGDVSAIDVSKVVQLDDLELRD
                     LGPRDVKLKILAVSAEHNIDHAATADTINIAEARGGKMYPGNSAVGEVTEVGSDVTAF
                     APGDVVITHCNGDPDRFGFPLRIWAYDMPDSIGWYSKEAVVEDWQLIKAPLDSGLNLW
                     EIAALPLRAPTAYHLWRRAIGIYRVKVPYERQARLNVLSFGGGVGELFLMLAKAEGHN
                     AYFCAGSKERRDALEKHGIVGIDQKQFNRF"
BASE COUNT         3565 a         8665 c         8879 g         3464 t
ORIGIN      
        1 cagcgagttg gtgacgacga agacgctgga gaagcccatg gcgccggcgg cgatcatcgg
       61 gacgagcagg ccgaaggcgg ccagcgggat ggcggcggtg ttgtaggcga aggcccagaa
      121 caggttgccc ttgatggtgg agagggtgcg acggctgagg gcgatggcgt cggccacgcc
      181 tcgggggtcg ccgctcacca gcgtgaggtc ggaggcctcg atggccacgt cggtgccggt
      241 gccgatggcg atgccgagat cggaggcggc gagcgccggg gcgtcgttca cgccgtcgcc
      301 caccatggcc acgacccggc cggcgtcctg caggcggcgc acctcggcga gcttgtcgtc
      361 ggggagcacc tcggccagca ccgtgctgat gccgacctgg gcggcgacgg cctcggcggt
      421 gcggcggttg tcaccggtga ggaggaccgt ctcgagcccc atctcgcgca gggcggcgac
      481 ggcctcgacg gcgctgggct tcacggtgtc ggagacggcg agcacacctc gggcccgtcc
      541 gtcccagccg gcgagcaccg cggtgcggcc ctcggcctcc tgggcggcag cagcggcttc
      601 gacctcggcc gggacctcgt cgaagagcat gcgacgtccg accaggacct cgacgccgtc
      661 gaccgtgccg tggacgccct tgccggcgag gttgcggaag ccctcgaccg ggcggtggtc
      721 ggtggtgccg gcggcgatgg ccgctgcgat cgggtgctcg ctcatggcct cgagcgaggc
      781 ggctcggcgc aggagcacgg cgtcttcgac gtcgggatcg aacgactcgg ccgacaccag
      841 ctgcatgcgg gcttcggtga gcgtgccggt cttgtcgaac acgacggtgt ccacggagcg
      901 ggtgcgctcg aggatctcgc cgcccttgat gagcacgccc atctgggcgc cacgtccggt
      961 gccgaccatg atcgcggtgg gggtggccag gccgagggcg caggggcagg cgatgatcag
     1021 gacggcgacg gcggcggtga acgcgtcgtc ggcgccgtgg ccggtgagga accagccggt
     1081 gagcgtggcg gtcgcgatca ggagcacgat cggcacgaag accgccgaga tgcggtcggc
     1141 caggcgctgc accggggcct ggctgccctg ggcctcctcg accatgcgga cgatctgggc
     1201 gagggcggtg tcggcgccga cccgggtggc ctcgaccacc aggcgcccgt cggtgctgac
     1261 ggtggcgccg atgacctcgt cgccgaccga cttgtcgacg ggcacgggct cgccggtgag
     1321 catggactca tcgagggcgg acgccccgtc gacgatgcgg ccgtcggtgg gcaccttctc
     1381 gccgggcttc acgatgaagc gcatgccgac ggcgaggtcg tcgacggcga tctcggtgcc
     1441 gtcctcgagc gtggcggtgc gggcgcccag gtcggcgagg gcgcggatgg cctcgccgct
     1501 gcggcgcttc gcccgggcct cgaaccactt gccgagcagg atcagcgtga tgatgacggc
     1561 ggccgtctcg aagtagacgt gggcgtctcc ggtggcgggg tcgcccatgc cggccatgtc
     1621 catggtgctg tcgccggccc cgaggaagat cagggcgacg gcggaccaca ggtaggcggc
     1681 gagcgtgccg atggacacga gcgtgtccat cgtggcggcg ccgtggcgag cgttgagcag
     1741 cgccgcccgg tggaacccga tgccgctgac gaacacgacc ggggtggtca gcgcgaacac
     1801 gacccaggcc cagccgtcga actgcagggc ggggatcatc gagagcagca cgacgggcac
     1861 gctgagcacg gcggcgatca ggaggcgccg ggtgatcgtg tggatccact cggcctcggc
     1921 tgcctcgtgg tcggcttcct cggggacgga gtagcccagc ttggtgatga cggcctcgaa
     1981 gtcgtcggcc gccagctgct cgtcgtcgaa gatcacggtg gcccgggccg atgccaggct
     2041 cacgttggcg ttctcgacgc cgtcgagctt gttcagccca cgggagatgc gagcggagca
     2101 cgccgcgcag gtcatgcctt cgacggggag atcgaccctg gtgcgcatgg cgtcaccgta
     2161 ccactgctgc gtgatacccc caacccctat ctggtagcat ggcgagatgc ccggctacac
     2221 gatgaacaag gaccagtacc aggcccgcct gcgccgcatc gagggccagg tgcgcggcct
     2281 gcagcggatg gtcgacgagg acacctactg catcgacgtg ctgacccaga tcgcgtcggt
     2341 caccaaggcc ctccagggcg tggggatggg gctgctcgac gagcacatcc gtcactgcgt
     2401 gcgcgaagcc gccgagctgg acggaccaga tgccgagcag gccttcgacg acaaggtcca
     2461 cgaggtcacg gccgccgtgt cccgcatgct ccggacctga gctcgcgccg acccctacgg
     2521 tgcgcgcatg cacctcggcg tgatgatgtt ccccacggac aaggccatcg atccggtcac
     2581 cctggcccgg gaggtcgagg cccgcggctt cgagtcgctg tggttccccg agcactccca
     2641 catcccgacc tcacgtgtga ccccctgggg tggccgggag ggtgcaccgc cgctccccga
     2701 ggagtactgg cggacccacg acagcctcat cagcctcggc gcctgtgccg ccgtcacgtc
     2761 cacgctcaag ctcgggacgg ggatctgcct ggtcgcccag cgcgacccga tctggctggc
     2821 gaaggaggtg gcgacggtcg accggatctc ctccggccgg ttcctcttcg gcatcggcta
     2881 cggctggaac aaggaggagc tcgcccacca cggtgtgcgc tacggcgacc ggcgcgacgt
     2941 gctgcgcgag cgcgtgctgg cctgcaagca gctctggacc caggacgagg cgagcttcga
     3001 cggtgagcac gtccacctcg agccgtcgtg gtcctggccc aagccggtgc agcagccgca
     3061 cccgccggtg atcctcgggg gagccgccgg tccgaagacg ttcgcccaca tcgccgagtt
     3121 ctgcgacggg tggatgccga tcgccgggcg tcacgccctc gttgaccagc tcgacgacct
     3181 gcgtgcggcg tgcgaggcgg tcgaccgcga ccccgccacg gtggagctgg gccagttcgc
     3241 gacccccgct cgtcccgagg cgctggagga gctggcgtcg gctggtgtct cccgagcggt
     3301 cttcggtctg cctcccggcg atgccgacac ggtgctgccc ctgctcgacc gctacgccga
     3361 gctgatcgac gccaccccct gacccccgct gctcccgcac cggcatcggt gctccaggtg
     3421 gcggctgcgg gtgccggtgc ccgagaatgg cgccatgtct gacgtgcagc gacgtgagcg
     3481 tgaccgcccc tggatgatgc ggacctactc gggtcactcc acggcgaagg ccagcaacga
     3541 gctgtaccgc acgaacctgg cccgggggca gaccggcctg tcgatcgcct tcgacctgcc
     3601 cacccagacc ggctacgacc ccgaccacgt gctcgcacgc ggtgaggtcg gcaaggtcgg
     3661 cgtgccggtg gcccacctcg ggcacatgga cacgctgctc gacggcatcc cccaggacca
     3721 gatgaacacg tcgatgacca tcaacgcccc ggcggcgtgg atgctcggcc tgtacgtcgc
     3781 caacgccgag aagaagggca tcgagtccaa ggcgctgcgc ggcaccacgc agaacgacat
     3841 cgtcaaggag tacctgtctc gggggaccta catcttcccg ccggtcccga gccgccggct
     3901 gatcgtggac atgatcgcct tctgcgccga gtggatcccg aagtggaacc ccatgaacgt
     3961 gtgcagctac cacctgcagg aggctggggc gacgccggtg caggagatcg cctacagcct
     4021 ggccaacgcc atcggggtcc tcgacgccgt gaaggagtcg ggtcaggtcg acgacgaccg
     4081 cttcgctcag gtgttcgggt ccatctcgtt cttcgtcaac gccggcatcc gcttcgtcga
     4141 ggagacggcg aagatgcgtg ccttcaccca gctgtgggag cgcatcggcc gcgagcgcta
     4201 cggcgtcacc gacgacaagg ccctgcggtt ccgctacggc gtgcaggtga acagcctcgg
     4261 cctcaccgag cagcaggccg agaacaacgt gcagcgcatc gtcctcgagg ccctcggcgt
     4321 cacgctgtcc aagagcgccc gggcccggtc gatccagctg ccggcgtgga acgaggcgct
     4381 cggcctgccc cgcccgtggg accagcagtg gtcgctgcgg gtgcagcagg tgctggccca
     4441 cgagaccgac ctgctcgagt acgaggacat cttcgacggc tcccacgtga tggagggcct
     4501 caccgccgag ctcgtcgagg cgtcgtgggc ggagatgacc gacatcctcg acctcggcgg
     4561 cgcgttcgag gccatcgacg agatgaaggg ccgcctcgtc cgcagccacg ccgagcggat
     4621 ccggcgcatc gagtccggcg accaggtcgt cgtcggcgtg aacgcctaca ccgagaccgc
     4681 accgtcgccg ctcggcggcg aggactcgat catgaaggtc gaccccggcg tgcaggacat
     4741 gctcatcgcc gacgtgcagc agtggcgctc cgagcgggac agcgacgccg tgaagcgcag
     4801 cctcgacgag ctgcggcggg tggccgagtc gggcgagaac atcatgccgg ccaccatcga
     4861 cctggctcac gccggcggca ccaccggcga gtggtcgggt ctgctgcgcg aggtcttcgg
     4921 cgagtaccgg gcccccaccg gcgtggccgc cgccgccggc cacggcacca gccagggcct
     4981 gcgcgacgtg gccgagaaga tcaaggctcg cccgtcgggc ccgcctcgcc tgctcgtggc
     5041 caagcccggc ctcgacggcc actccaacgg cgccgagcag atcgcggtcg cggcccgcga
     5101 cgcgggcttc gaggtgatct accagggcat ccggctcacg cccgcccaga tcgcccaggt
     5161 ggccgccgac gaggacgtcg acgccatcgg gctctcgatc ctgtccggca gccacctgga
     5221 gctggtgccc gagacggtgc gcctcatccg tgaggccggc agcgacgcgc ccgtcatcgc
     5281 gggcggcatc atccccgagg acgaccgcag cgtgctcacc gacgcgggcg ttgcggccgt
     5341 gtacacgccg aaggacttcg acctcaccaa gatcatgggc gagatcctcg acctggtcag
     5401 cgattgaaca tgggctggtg ggggtgagcc gggcgggggg tcgctggacg gtcgtggcgg
     5461 cggccgtggt cgccggcgtg gcgggcgtgc tcgccgtgct caccgcggca gccgtccccg
     5521 cagtcgtcgc cggcatcgcc agcgccctcg tggccgtgct ggtcctcatc gacgatccgg
     5581 aggagcacga gcccgcacca ccgccggccc cgcccgccga ccccgtgccc gaagcaccgg
     5641 aggccgacga ggacatcgac cccatccggg cgatcttcga atccggtccg cccgccggcg
     5701 gcagcggcat gctcagcccc gagttcctgc tcaccaccct gcggggccgg gtggcggtgg
     5761 cccggcgggc gctccggccc ctgtcgatcg tgtgcttcga ggcctacgag accgatgccg
     5821 acggggcgac cacgtccccg gttgcggccg aggacgtggg tgcggtgctg cgccgcacgc
     5881 ttcgggaggc cgacgtggcc ggccacctcc acggagacgc cttcgtctgc atcctggagg
     5941 acaccggcga gaacggggcg gtctggaccg ccgaacgggt gcgccgcaac ctcctcgagg
     6001 gcgggcaggc ccgtcggttc cgggccggcg tggcgtccta cccggcccat ggcctcgagc
     6061 ccgaggagct cgaggcgaag gcccgggcgg cgctcgtcgc ggcgcgggac tgggcgacgg
     6121 accgcatcga ggtcgccggc gcgccgtgat cagtcgctga gctcgtccag cacctcgtcg
     6181 gtgtcggcgc cgagcgggcg accggtccac cggatgcggc cgggggtggc ggacaaccgg
     6241 gcgacgaggc cctgcatcgg ggtgccatcg acctcctcga ccgcgccccg ggcccgcacg
     6301 tggggatcgg ccaggacctc gttcatggag tacaccggcg cgcaggcggc gtgggcctcc
     6361 tcgaaggcgg ccagcacgtc gtctcgggtc cgctcgccga tccactcggc catgcgggcg
     6421 tcgatctgct cccgggccgc gacccggccg tcgaaggtct gcaggcgctc gtcgtcaccg
     6481 agcccgacga ggtcgagcac tcgacgagcc acggactcgg ccgaggtcga caccgccacc
     6541 cagccgccgt cggcgcactg ccacgtcccc cgcgggacgg tgtaggggac gcccgacccg
     6601 agccggggtt gctcccaccc ctccaccgca gacgccgaga ccagcggtcc catgatctgc
     6661 agcaacgact ccagcaggtt cacgtccacc acctggccga ccccggagtg gaccgcgacc
     6721 atcgtggcga aggccgcagt gaggccggtg acctcgtccg tgagcgcgat gggcggcagg
     6781 agcggggcac cgtcgggctc gccggacagc gaggcgaagc cggacatggc ctcggcgagg
     6841 gtcgcgaagc cggcgcggcc cgcgtagggc ccgtcctggc cgaacccggt gacccgggtg
     6901 atcaccagat cggggttgcg ctcgtggatc acgtcgggac cgaggccgag gcgctcgagc
     6961 gtgccggggc ggaagttctc caccagcacg tcggcgctcg cgcacaggtc gagcatgtgc
     7021 ccccgcccct cgtcggactt gaggtcgagc acgatcgtgc gcttgccgcg accgagcagc
     7081 ttccagaagt aggtgacgtc gtcggcggga tcgcgacgga acatgtcgcg ggtgccgtcg
     7141 ccgctgccgg ggcgctcgac cttgatgaca tcggcgccga agtcggcgag gtagcgggcg
     7201 cacgccggac cggcgaggac ggtggacgcg tcgatgaccc gcaggtcggc gagaggcagc
     7261 tgggctgtca ccgctccagc atgaagcacc ggtcggcgag cctcccaaag gcgtagtcgg
     7321 cgtacatgcc gaggtacatg tgccgggtgc gctcggtcca gtccagcgcc tcggggctgg
     7381 agatccggtg gtgcagctgg acgatgccgc cctcgtgcac ccggtactcg gcccaggccc
     7441 ccgggtagtc cttcacgcag gccacctcga cccagggcat ggccccggtc tgggggaagc
     7501 gccgcacgcg gttccggtgg gtgtggccgg cgaggtagcc gacgatgctc ggccggcggg
     7561 ccgcgacctc gatcaggcgc tcggaccagt ccgggtggat cccgaagtac gtctcgttcc
     7621 gctccttcga ccgagggtcc cacacgtggt ggtggccgag gaccacgacc gggcggtcgg
     7681 cgtcagcggc gatggcgtcg agctcgtcga gctggtcgtg ggtgagcccg ccggaggact
     7741 tgaagggcac cgacgtgtcc agcagcacga ccgtggcgcc gggcaggtcc acccgctgga
     7801 gcggccagtc ggcgtaggac tgcccctcgt acgcgtcgtg gttgccccgc acgtgggtga
     7861 gccgatcccc gaaggcgccg ccgtaggtgt cgaggaaggc ggcgtactcc tcgtcggtgc
     7921 cgtcgttggt gaggtcgccc ttgacgaaga cggcatcgac ctcggcggcc tggatctcgg
     7981 cgatggctgc ctcgttcatc gtgaccgggt acgggggctc gccgggctcg gccgtgagga
     8041 tcggaccgat ctcgtcctcg tggccctcca ccacgccgca ccgggtctcc ccgaagtgca
     8101 cgtcgttgac ggtggcgaag cgggccagga gctcgccgcc ggggtgcggc atggtctcga
     8161 tctcgaggcc gtcgaggtcg tggacccgac cagcctccag gtcgtcgtag cgtcgcacgt
     8221 cggtgccgcg gtggacgacg accaggtcgg gcccgaccgt ggtgatctct gggcccacgg
     8281 gcgcgactac caggggcggt tgcggcgcat cgggaccggc tggctccacc agcgccccga
     8341 gaaccgccac cggttcgggc ggtcgtcggg ggtctcgccg cccacccggg gccaggcgac
     8401 ctcgacggcc gcggcgatgg ccacggcctc ctcatcggtt ggtgcgggct ggatctgcac
     8461 ggaggcgaag ttactcgggg tggcccacgg cgacaccagt gccggggtcg aacaggtgca
     8521 cccgggacgg gtcgacgacg agctcgatcc ggtcgccgac gcgggtgctg gtgggtgcgt
     8581 cgtcccagcg caggaccccg ccgtcgaccg tgacggtggc gatcgcccgt cgcccgacga
     8641 gctcgacccc cacgacgacg gccgggaacc ggatgccggt ggttccgggg gtggcgaggc
     8701 gcagctcgtg gggtcggatg ccgacgtcga cctcccgtcc gtcgagctcg gtccgtacga
     8761 cgccgatctc cggttcgccg aagaaccggg cgaccgccag ggtgcgcggc cggtcgtaca
     8821 cgtgcatcgg cggccccgcc tgctccaggc gaccgtcgat cagcacggcc acccggtcgg
     8881 cgacggcgag cgcgtcgcgc tggtcagcgg tgacccagat cgtggtgacg ccgtacagct
     8941 gctggaggcg cacgaggtcg gcgcgcacca tcccgcgccg cgccggatcg atccgggcca
     9001 gcggctcgtc gaggagcagc accctcggcc tggcgatgac cgcacgggcg atctgcgcga
     9061 cctggatctc ccccccgctg agggtgctcg gggggcggcc gagcacgccg ccgaggtcga
     9121 gggcgagggc ctgctcgtcg atgcggaacc ggcgctcgtc ccggtcgatg tccctgcgca
     9181 ggtcgagcgg caagccgacg gagtcctcgg tcggccggga cggcagcagc gccgacgcct
     9241 gggtgaccat gccgagtcct cgatcgcggg tgggtacctc gttcacccgg cggtcaccga
     9301 tccggatctc gccgctggtc ggcgcctcca gcccggccag caggcgcacg gccgtcgact
     9361 tcccgcaccc ggacggcccg accaggcagc agatggtgcc gtcgtcgacg tccaacgtca
     9421 gcgaccggag ggcggttcgc ccggcgcgct ccatggtcac ggcggcgaag gcgacggatg
     9481 ccatcaggcg tccctgcgct ccacgagcac gaggccggcg ccggctgcgc ccacgaccac
     9541 cccgccgacg atcccgagga cgccggcgct ggtggcgtcg agcaggacgc caccggccag
     9601 cgggccgacc acccgcccgg cggccatgac cgcctgcgcg tcacccgccc gctcgcccgg
     9661 gttggcggat cgcgcggcga gcagcgagaa ggctcccggc gtccccatcc agaacgcgaa
     9721 gccccagaag gcgatggcca ccacgaacag cggcccgctc gtcgccgcac ccatcacgac
     9781 cgcgcagacg ccgcacccga cgatccacgc gcccgccggt cctcgccgtc cggaccaccg
     9841 agcgctcggg atcgaggcca gggcgttggc gctgtacgcg agggagatcg ccgagagcga
     9901 cagcgtggtg cggtcggcgg cgatcacggc cccgaacgtg aacaccgacg agccgcccac
     9961 cgtcatgagc ccgagcagga gcaggatcgg ccggacgccc ggtgcgggcc gggagcggga
    10021 cccggcggcc cgctcggccc tcggggtgac gatgcgctcg tggtcgctgc cgctgtggcg
    10081 gctgaacgcg aggggcacga gggccgtgac ggccagggcc acgaagatgc cccggggacc
    10141 gaaccggtcc accagcgctg cggcgatcgg cgccgtggtg atcccgatga tcggtccgac
    10201 caccgcgatc tcgctcatcc gctggtcgtc gccgaacacg agggtccagc tgaaccaggt
    10261 gatgagcccg agggcgacgc cgctgacgac ccgcaggccc acgagcacgc cgaaaggcgg
    10321 cagcagggcg ctgagcccgt gggcagcgac cgagacgagc agcgcgaggc cgagcacccg
    10381 gccgcgaggt cgcaggaacc gtccggcgcc ccacgaaccg gcgacgaagc cgagcagctg
    10441 ggcggcggtc gcgaacgacg ccaggccgag cccgatgccg tactcgtcgc tcagcgccgg
    10501 gagcaggaac ggcgccaccg acagcacggc ggtgccgtag gcggtggcga gcagcaggcc
    10561 cgggtgctcc gacggccgga tcgcccgcag gagccggatg gggacctgca cggtggcgga
    10621 ccgtaccggg cgtctccggt gtggtccgtg gccggcgcta cagcggcacg ttgccgtgct
    10681 tgcgcgccgg cagctcctcg cgcttggact cgagcatgtc gagggcggcg ttgaccttgc
    10741 gtcgggtgtc ggccgggtcg atcacgtcgt cgacgtagcc ccggtcggcg gccgcgtagg
    10801 ggttgaggaa cttctcggag tactcctcga ccagctcggc cttcttggcg tcggggtcgt
    10861 ccgagtcgag gatctcgcgg cggttgacga tctccacggc accggcggca cccatcaccg
    10921 ccagctcggc cgagggccag gcgatggcgt agtcggcccc gatcgagcgg gagttcatga
    10981 ccacgtaggc gccgccgtag gccttgcggg tgatgatctg gatccgcggc accgtggact
    11041 cgcagtaggc gtagagcagc ttggcgccgt ggcggatgat gccgccgtgc tcctggtcga
    11101 cgccgggcat gaagccgggc acgtcgacga acgtgagcag cgggatgttg aaggcgtcgc
    11161 aggtccgcac gaaccgggcc gccttctcgg aggactcgat gtcgagcacg ccggccatgg
    11221 ccatgggctg gttgccgacg atgccgacgg gccgtccggc catgcgggcg aagccgcagg
    11281 tgatctgggg tgcccaggcc ggcgcgtact cgaagtagtc cccgtcgtcg acgatctcgg
    11341 cgatgaccgc cttcatgtcg tagggctggt tcgggttcgt cggcaccatg tcgcgcagcg
    11401 cgtcgcactg acgctcgggg tcgtcgccgg tgttgagcac cggagggacc tcgaggttgt
    11461 tcgacggcag gaaggacagc aggtacttga cgtcgtcgag cacggcccgg tcgtcggggc
    11521 cggtgaactg gcagacgccg gacttctgcg agtgggcatc ggcgccgccc agctcctgct
    11581 gggtcacgtc ggcgccggtc accgtcttca ccacgtcggg gccggtgatg aacatgtagc
    11641 tggtgtcctc gaccatgaac acgaagtcgg tcatggcggg ggagtagacg gcgccaccgg
    11701 cgcacgggcc catgatcacg ctgatctggg gcgtgacgcc cgaggccagc acgttccggt
    11761 agaagatccc gccgtaggag tcgagggaga ccacgccctc ctggatgcgg gcgccggcac
    11821 cgtcgttgag gccgatgagc ggggcgccga ccttcagcgc catgtccatc agcttgtgga
    11881 tcttgttggc gaacacctcg ccgagggcac cgccgtacac ggtgaagtcc tggctgaaga
    11941 cgaagacctt cttgccgtcc acggtgcccc agccggtgat gacgccgtcg gagtaggggc
    12001 gctcgtcgaa ggaggactcc atgggccggc gggcgagcag gtcgagctcg tggaagctgt
    12061 cctcgtcgag gaggtactcg atgcgctcgc gggcgagcat cttgccgcgc tcgtgctgcc
    12121 gggcgatcga gcgctcggac ccgggctggc gcgcctcggc cttcttctcc tcgaggtcag
    12181 cgatgcgctc ggacatgggg tgctcggcca tgggcgagga aactagtggc ggcccttccg
    12241 ggggtcccac tcccgcgcga cctgtatccg ttcgtccggt ttcgtactgt cggtgccgat
    12301 cgtcctgatc gccgcaccac agggggaccc atgagaaaga gcccggacgt cagccgtcgc
    12361 cgcttcctcg gctacgtcgt cgccggcccg acgctgatca ccgccgcgag gtggatcgat
    12421 ccgctcgcca cggccgacgc gcaggagctg ccctcgatcg ggccccagat cgccgagtcc
    12481 tacgacctca tcgacttcct gcgcgacgcc gcacgaccga cggccaacct gatcacggtc
    12541 acggtggagg acgacggcac cgtgtccttc gagctgcacc gcaccgaggt ggggcagggg
    12601 atcaacacgg cgatcgccat gatcatcgcc gaggagatgg acgtccccct ctcgcaggtg
    12661 cgcatcaccc aagccgatgc acgccccgag ctcctcttca accagctgac cggcggatcc
    12721 aacagcatct acaccctcta cgaccccgtc cgcatcgccg ccgccgtcgc ccggcagcgg
    12781 ctgctcgacg cggccgccat cgccctcggg tccgccggcg ccgagctcgg catcctcgac
    12841 ggggtcatcc gcgcccctga cggcagctcg atcgggttcg gcgagctcgc ccgcgacgcg
    12901 gcgtcgcagg tcaccgaggc cgtcgacgtc cagccgaagc gggagcgtcc ccgcctcgtc
    12961 ggcacgccgc agaaccggat cgacgcccgg gccatggtca ccggcgagct ccggtacacg
    13021 ggcgacctcg tggtgcccga tgccctcccg acgatggtcg ctcggccacc cacgcacctc
    13081 ggccggccgg tccggctgcg caacgaggcg gcggtgctgg ccgtcccggg ggtgacccac
    13141 gtcgcgctcg tggagagcgg cgtcgccgtc cgcgcccgca ccttcggcca gtgcatcgac
    13201 gcgctccagc tgatcgacgt cgaatgggaa ccgggtccgg ccgcggggat gtcggacgag
    13261 tcggtccagg ccgcgctcgc cgacgccgag ctgccccaag ccgttccgga cacgggcgcg
    13321 atcggagcgc tgggcatccc gtcaccggtg cagacgaggg acttcacctt caccttcgcc
    13381 ttccaaccca actgcccgct ggagacgaac ccgtgtgtcg ccgacgtgcg ggccgatcgg
    13441 gccgagatct gggggccgat gaagaacccg gtcgtggccc agcaggacat cgccgacgca
    13501 ctgggtctgc cccaggacgc cgtcaccgtg cacgtcgtgc agggtggcgg gtccttcggc
    13561 cgggatctct tctgggacgc agcgcgggag gcggccctcg tctcccaggc catgggggtg
    13621 ccggtgaagc tcagctggac ccgcacggac gacttccgcc agggacgagc gcacccggca
    13681 tgtcgctcgt cgatccggat cacccgcagc gacaccgaca tcgtctccta cgcccagcac
    13741 cacaccagcg tgaacaccga cttcgggcac ggcctcggcg agatcatctc ggcgcgggtc
    13801 gatgagctgc cgggcgggct cggcggcatg ggcttctccc agaccgtgtt catgctcacc
    13861 cagtacgtcc cgtacgactt cggtgcggtc gagcagctcc tgaccgagac gcttccgtcg
    13921 ccgggcgaga tcgagacgaa cgtgttccga accggctcga tgcgaaacat ctacagcccg
    13981 aacgtggtca cggcccgtga gctggccgtc gatcaggtgg cggccgaggt cggccgcgat
    14041 ccgctcgagc tgcgcctgac cacgatgcgc gacgcccgct cgcgggccgt gctcgagaag
    14101 gtcgccgaag ccggcggctg gggccgggag atgactcccg gtaccgccca gggcctcggg
    14161 ttccacagcg agtacaaggg ccgggccgcc tgcctggtcg agatcgactg ccgccccgag
    14221 accgtgtccc gcaccgtccc gacggcggcg tacgcctaca cgggcccgcg agtgacccgg
    14281 gcgatctacg ccgtcgacgt cggcctcacc atcaaccccc gagggctcga agcccagatg
    14341 ctcggcggga tgatggatgg gatcgccaac tgcctgtcgt tctcgttgca cctggtcgat
    14401 ggggccttcg ccgaggactc ctgggagcac gcgtactaca cccgccagtg gaacgtgccc
    14461 ttcgagaccc aggtgttcat cctcgactcg tccagccagc ccggcggtgc cggcgagttc
    14521 ggcgtcgccg cctccatggc cgcgaccgcg aacgcctacg cccgggccac cggttcgatg
    14581 ccgacgtcgt tcccgatcaa ccacgggatg cccctcgccg agtccatcgt gccccagggc
    14641 accatcccgc agtcccccac cgacggcctc ggccggcgct gagaggagca tcgatgcctg
    14701 tccagaagtt cacggtgaac ggccagcggg tggaggtcga cgtcgaggac gacgtgcgcc
    14761 tgctctgggt gctgcgcgac ctgctcgacc tcaccgggcc gaagtacggc tgcggcctca
    14821 gcatctgcca gtcctgcacc agcctcgtca acggcaaggc gttcaacccg tgctccgtcc
    14881 ccgtcggcca gctgcggccg agcgacgagg tcatcaccat cgagggcctg gccgacgcag
    14941 tggacggcga cggcctgcac ccggtgcagg aggtctggct ggaggaggac gtcgcccagt
    15001 gcggcttctg ccagcccggc cagatcatgg ctgcggtcga cctgatccag acgctgctcg
    15061 ccagcggtcg cagtgacgtg accgaggccg agctcgactc gctccgcaac gtgtgccgct
    15121 gcggcaccta cccccgggtg cgcaccgcca tccgccgcgc cctgaccgtc atggcggaga
    15181 ccccggtctt cgtgcgcggc gccgggcgat agcgacgccg gtaccttcgg ccggcgtgca
    15241 ccggctcttc gtctacggca ccctccagca cccgccgctc ctggagcacc tcctcggtcg
    15301 atcgccggcg ctcgaggccg ccaccgtcgc ggggtggcgt gcggcgcgtc tgcgtgggcg
    15361 ggtgtacccc ggtctggtgc ccgctccggg gtcctcggcg cacggccacg tcatcgaggt
    15421 cgacgacgac gagctggtcg tgctggatcg cttcgagggg ccgcagtacg agcgcatcgt
    15481 cgtctccgcc gacggccacg aggtcggtgc ctggcgcctc cgctccgagc acgtcccctt
    15541 ggccctcgag gaggactggg acctcgaccg cttcgtcgcc gaggacgccg tcgcgttcct
    15601 cggcgcatcc cgtcccggtg acgagcatcc gtgggacggt cagcggtaga ggagcgcgcc
    15661 gtcgtcgagg tcggcggcgg ggaactcgtc cttctccgcc cagtagtcct gggtgaagcg
    15721 ccacggctcc ttcgtgccct gcttcggcat gaggtggagc gcccgcatga ggtagccggg
    15781 gttgaagtcc tccgggtcga cccagggcag caggcccatg tcggcatcct cctcgcgcag
    15841 ctggggcacg acgatgtcgg ccccgagctc gtccatgtgg tcgagcaggc ggcacacgaa
    15901 gtcggcgatg aggtcggcgc gcaacgtcca gctggcccgg aagtagccga agacccacgc
    15961 catgttcggc acgccggtga acatcgtccc ccggtacccg acggtctggg cgaagtcgag
    16021 cggctcgtcg tcgacggtga aggcgatgtc gccgagcacg ctcatctcga agccggtggc
    16081 ggtgatgatg aggtcggcct cgagcacctc gcccgactcg aggcggatgc cctcggggac
    16141 gaaccggtcg atggtgtcgg tgaccaccga ggccttgccg ctcttgatcc cctcgaacag
    16201 gtcgccgttg gggacgaagg cgatgcgctg ctgccagggc cggtacgccg gggtgaagtg
    16261 cgggctcagg tcgtagtcgt cgccgaggat cgccttgatc cccttgaaca gctcatcctt
    16321 caccagctcg gggtgctcgg ccgacatgga ggtcaccagc gcctgggtct tgagcacctg
    16381 gcgacggacg atctcatggg tccactcgtc gggcacctcg aggtcacgca gctggtcggc
    16441 cagctcgttg gcgttgcggc cggtggcgaa gtacgtgggg gagcgctgca gcatcgtgat
    16501 gtgctcgcag tcgggcgcca tggccgggat gagggtggcg gcggtggctc cggagccgat
    16561 gaccaccacg cgcttgtccg cgtagtcgag gtcgtcgggc caggtctggg ggtgcacgat
    16621 ctggccggtg aagtcgtccg tgccgtccca ctccggggtg tagccctcgg tgtggcggta
    16681 gtagccctgg cacatccaga ggaagccggc ggagacggtg aacgcctcgc cggtgtcggt
    16741 ccgggtgccg gtcacgatcc agcgccggtc ctcggtggac caggcggcgg tgtcgatctg
    16801 gtgctggtac cggatgtgct cgtcgagccc gtcgtcctcg atgacctcgc cgaggtaggt
    16861 gaggatctcc tgggcggtgg cgatcggcgc gccggtccac ggcttgaacg agtagccgaa
    16921 cgtgtacagg tcgctgtcgg agcgcacgcc cgggtaggtg tgcgtccgcc aggtgccgcc
    16981 gtacccctcc tggccctcga ggatcgtgaa cgtggtgccc ggccggtcgg cgagcaggtg
    17041 gtgtgcggcg ccgatcccgg agatcccggc tccgacgatc aggacgtcga cgtggtcgtt
    17101 gggcatgcct tcccccttcg gtggtgcgcc atcgtgcatc gcgggaccgc cccgggagga
    17161 accgacgaac gacccgtagc ctcgggtcga tggacctccc ggtgccgttc cccctcaagc
    17221 cgatgctcgc caaggcgaac gacggcatcc ccacgggtga ctgggtcttc gagccgaagt
    17281 gggacgggtt ccgctgcctc gtgttccgcg acggtgacga gatccagctc gggagccgca
    17341 acgagcggcc cctcacccgc tacttccccg agctgctcga ccccctgcgg gctgcgctcc
    17401 ccgagcgttg cgtggtcgac ggcgagatcg tcgtcgcgat cggcgaccgc ttggacttcg
    17461 acgccctcgg ccagcgcatc cacccggccg agtcgcgggt gaacatgctc gccgagcaga
    17521 cgccggctgc gttcgtggcg ttcgacatcg tcgcgctcga cgaccgagcc ctgctcgagc
    17581 tgcccttcgc cgagcgacga gcgctgctgg agcaggcgat caccccgggc gcgcaggtcc
    17641 acctcacgcc cgtcaccgtc gatccagacg tcgccgccga ctggttccgc cgggtcgagg
    17701 gcgccggcct ggacggcctg atcgccaagg acccggccag cacctaccag cccgacaagc
    17761 gcatcctgcg caaggtgaag caccagcgca ccgccgactg cgtggccgcc ggcttccgct
    17821 ggcacaagga cggcaagggc gtcggctcga tcctgctcgg catccacgcc ggcacccccg
    17881 acgacccggg cgacctgcgc cacgtcggcg tctcggcgtc gttcaccgcc aagcgtcgcg
    17941 ccgagctcgt cgatgagctg gcgcccttcg tcatggaccc cgggacggac gagttcgccg
    18001 agcacccgtg gagcagctgg tacgacgccg aggcccacga gaccggccag ctcccgggcg
    18061 cgccgaaccg gtggaacagc caacgggacc agtcctggat cccgctgcgc accgagcggg
    18121 tcgtggaggt cggctacacg atcatgacgg cgggccgctt ccgcgggacc acgcgcttcg
    18181 tgcgatggcg gccggacaag gaaccgcacc agtgcaccta cgaccagctc gacgtggtgc
    18241 cgccgatggc cttcgccgac gtgctcagcc cgcctgacgc ctgaccagcg tcagcgtcgt
    18301 gccaagcggt ctgctcgttc tgccaggcgg tcgccgtcca gcgatgcggc cacgtcggcg
    18361 aagccgtccc ggggtcctcg ccaggtcatg tcgtccaccg tggcgctgat gggcgcgtcc
    18421 ggctgcagcg tggccagcgt gcggaacagc agggcgtcgt cgagctggtc ctggaggacc
    18481 ttcgccagct tcgccccgct gcgcacggtg atgtcccact gcccggccga cggcgggatg
    18541 tcctcgatgt gcacgtagcg cgccagcacc gccgcagcgg acttggcgcc ccaccccggc
    18601 aggccgggga acccgtcggc cgagtcgccg acgagggcga ggtagtccgg gatggactcg
    18661 gggaggacgc cgaacttctc ctgcacgcca gcggtgtcga accacttgtc ctggcggcgg
    18721 tcccactgca cgaccttgcc gccgacgagc tgggccatgt ccttgtcggg cgtgcagatc
    18781 accacccgct ccacgtcgtc gtcggctgcc gcgagcacgg cgccggcagc caggccgtcg
    18841 tcggcctcga acgtggtctg gggccagacc gcgaacccgg ctgcagccag cacctcctcg
    18901 agtaccggga actgcgcctt cagctgtgga tcgatgccct cgccggtctt gtagtccgcc
    18961 cagaggtcgt tgcggtagct ctcgatgacg tggtcggtcg cgacgccgat gtgggtggca
    19021 ccgtcctcca ccagctgcag catcgagttc acgaccccgc ggttcgcgcc ccgctcgggg
    19081 tcgcggttgt tgggtgagta gtggtaccgg aagagctcgt aggtcccatc gacgaggtgc
    19141 acggtcaggc ccatcggcgc atcttcccag gcgcgcgacg gtcgcgagga cgtccgccac
    19201 ctgtcccaga ccctcgacgg ccgcctcgag ggcgacttcg acctgggcgc cgggcgctca
    19261 tcgcccgggt ccgaccgctg tcgccgcagc gtcagcgcga gggcacgccc gctgcgcgca
    19321 cgaggctgcg caccttcttg cggtgcgtgg tcaggtcgtc gagcgtccgt tcgtacgccg
    19381 cccgatccag cagcgggatg ccggcggcat cgagacggtc ggtgcgcggc gtcgtgggcg
    19441 tcccgtcccc gtcgacggcg ctcccgtagg tctcgaggta cgtctcgatg gcggagcgga
    19501 gctggcggcg tggcgtcgcg agcggtgccg cccgcggatc gaccccgtcg aggagggtga
    19561 gcagatcatc caggtcggcg acggcgagcg gtgcggacgt ggcggtgtcc gagctctgga
    19621 acaggtggag caccgggtac gccaggtgct gctcggcgag ggtgccgagc gccgtcgcga
    19681 ggtcccgcat ctggtggtcg agctggatgc gatcgccgtc ccacgcgccc aggaccacgt
    19741 cctcaggggt gtcgccgagc gagcgagcgg aagacgcgaa ggagcgggat gccaccgagg
    19801 cagagatgac ggggagcagg taggtcaccg acagggtgag caggaacagc ccggtcgcgc
    19861 tgactgccac ggcgagcaga cggccgaagt cggtgacggg cgcgaggtcg ccgttgccga
    19921 gggtgaagag gctgtagccg acgaagtagg cacgctcgaa ccagtcgacg ggctgcttcg
    19981 tgcccgaggt gcggatggca tccggctgcc cctccagcat cagggtgaac ccgatcagga
    20041 gcacggccgt ccacgtgacc acgacggcca ggagcgcgac cgggccgacg gcggaacggg
    20101 cccggcggtt gcccgacagg agcggcagca gtcgtcggcc gatccccgtg atggtgccgg
    20161 tcagcgggcc ggcgccctgg cccgaccaca gcacggtccg gaacacgtcg gccatgacca
    20221 ggaagacgag gaggcatccg aggcccaccg gcaggtagtc gcgcaccggt tgccactacc
    20281 ccggtctggt gcgggtgatg ttcacggctc cgggagcagc agctcacccg agccgacgcg
    20341 caccgctcgc ccgccgacgc gaacggcgac cagctcgttg ccgttgcgga ccgcctgcac
    20401 gcgcatctcg ctcggccgtc ccatctccac gccctgctcg atcgtccaac cttcgtccag
    20461 gcgtcgctct cccgccaccc cggccaggta gccgcacgca gcggcggcag cggagcccgt
    20521 cgccgggtcc tcggcgacac cgatgaccgg gccgaacatg cgggcgcgcc accgctcgcc
    20581 acgcaccccg tcgaccgggg ccagcaggta caggtccggg gcgtcggagc agccgacccc
    20641 gtgccgccag gctgcggggt cgagctcggc gcgctcgagc gcgccgacat cgcgcagccc
    20701 caccatcgtg tacggcacgc cggcgctcca gccacgggga ccgagctcgg ggtggaggtc
    20761 cgcgagtccg agtccgacgg cggcgacgac atctgcgggg tcggcggtgt cgacgggctc
    20821 cggtcgcccc ggggtggtga gggtggcgag gccgtctgcg agcgacacct cgaccggtcc
    20881 gatcccctcg tgcagggtca cgcgaccgtc gcggaggagg tcggcgtcct gcagggcgag
    20941 cgccgcgccg atggtcgggt ggccggcgaa ggggagctcg gtcgccggcg tgaagatccg
    21001 ggcccgccag gccccaccgc cgaggtcggt gaggaacacc gtctcggaga ggttgagctc
    21061 ggcggcgatg cgctgcatct gggcgtcgct gagcgccggc ggttcgatgg ccacggccag
    21121 tgggttcccg gcgtaggcgc ggtcggtgaa cacgtcgtag cggaccacgg tgagtcggtc
    21181 ggatcgaacg gcggccatgg ccaagcctgc gcggcccggg cccggcccgg ccagcgcgtt
    21241 catcgcgacg gtgggtcaca ccgactcgta cactcgccac gggggttccc gctgcgcgct
    21301 gccacggagg gtgatgaccg acaacgagcg accgcagggc agcaccgtcg cggcaggtcc
    21361 cccgacgtcc gccccgagcg gtgagctctc ggggatctgc cgacgcgtcg tgctgtggta
    21421 cgccgccgtg gcggccacct ggatcgtggt cagcgacctg acggtcagcg ccgtgcgcaa
    21481 cgagctcgcc gagcagcagg cggtgaacgt cgtcaagggg ctggcgttcg tgctcgtgac
    21541 gacggcggtg ctgtgggtcg tcctccggag gctcactcgg tcctacgacc agcacttcgc
    21601 ccgcgaggtc acggcccagc gcgacttctt ccagtcgatc ctcaacacgt cgacggacct
    21661 ggtgctgatc ctcggggcgg gcggggccat ccgctacgcc agccacgccc tgatcgaggt
    21721 cctcgggtgg gaccacgacg aggtcgtggg ccgtcgcggg cgagacttcc tccaccccga
    21781 cgacgtgagc tcggccagct cgttccgcgc caacctcggg ctgggcggcc ccaaccggcg
    21841 gaccttccgc atggcgcacc gggacggcac ctaccggacg ctggagatgg cgatgtcgac
    21901 gatctcactc ggtgacggca ccgagggagc ggtcgtcctg gcccgggacg tgaccgagcg
    21961 cgcacgtggc gagcgacagc tgcgggcggc gctggcggag gacgcgaccg gcctgccgaa
    22021 cctgcacatg ttcgtggccg agatggaacg tctcgccgag gtcaccaccg tcggtctcga
    22081 tgccgtcgtc ctgctcgtgg acatcgatcg cttcggggac atcaacgcgc tgcacggccg
    22141 ggtcggcggc gacgctgtcc tgctcgagct ctcccgtcgg ctggaagcga ccctccccca
    22201 ggcgatcggg ctctggcgcc acggtgccga tgagatcctc gcgctcctcc tcgaggaccc
    22261 cgccggcccc gacgcagtcg gcgaggtcgg gttggcccgg ctcgtcgagc gcgtccagca
    22321 cgaggctgcg gcgccgatcg ccatcgacga caccgatcgg cgcctctcgg tgagcgtctc
    22381 cgtcggcgtc gctcggctgc cgatcgactc cgcggtgccg gaggactcgc tgagctcacg
    22441 gatgctgcgc acggcagagg aggcgctcgc cgaagccaag cagcaccccg accgcagcgc
    22501 agtgcgcgtc cagcgtgacg cagcagcagc gggcagcgac cgcgccctcg tgatcgagca
    22561 gctgcacgac gcggtggcgc gcggggagct ggtcgtccac ttccagccca agatccgcct
    22621 ctccgacctc agcgtcgtcg gtgccgaagc cctcgtgcga tgggagcacc ccgaccgggg
    22681 gctgctcccg cccggggagt tcctccgaac ggtggacgag gccaacctca cggcggcgct
    22741 gacgcgcagc gtgctccggg atgcgctggc ccaggtcccc ggatggctgg gcgcggaggg
    22801 ctgcaacccg gacttcgagg tctgcgtgaa catcagcctc gacgacctgc gtcggcgccg
    22861 cttcgtggac gacgtgttcg acgcgctgca cgagagcggg gtcgaggcgc agcacctgtc
    22921 cctggagctg accgagcaga cgatgctcgc cgacgcgctc ggcgccagca ccgtcgtggc
    22981 cgagctccgc cgtgccggca tccgggtcgc catcgatgac ttcggaaccg ggttctccag
    23041 cctcgagcac atccgcatct tcgaggtcga cgagctgaag atcgacaagg gcttcgtgca
    23101 gcagatcggc cgcagccgga ccgacgaggc catcgtcgac tcgatcctgg ccatcgccgg
    23161 ccgcctcggc gtgaaggtca ctgccgaggg catcgaagcg ccagacgccc tggagtacct
    23221 ccgtcagcgc gggtgccacg aggggcaggg cttcttgttc agcgcagccg tgccggtcga
    23281 ggagttcgct cctggccggt cctgggcgac gctggcctag aggttctcct gcacgagctc
    23341 gatgagggtg ccgaggctgc ccttcgggtg gatgaacgcg acggtggttc cacgggagcc
    23401 gggccggggc gccttgtcga tgggcgtggc gccagcggcg accatggact ccagggcctg
    23461 ggcgcagtcg tcgacccggt agccgatgtg gtgcagcccc tcgcccttct tctccaggaa
    23521 cttggcgatg ggcgagtcgt cgcgggtggc cgcagtgagc tggatgtagg aatcggccac
    23581 cttgaccagg gcttcctcga cgccgtcgga gtccacgacc tcacggtggt cgacctcggc
    23641 gccgaaggcc tcggcgtagt aggcgatggc ggcgtcgagg tccttgacgg cgatggcgac
    23701 gtgatcgatc tcggtcagca gcatgacccg aaatctagtg cgatcggacc agccggaatc
    23761 ccatcaggac gtgccgatcc gacatgatga ccggctggta accgagctga ccggaggaac
    23821 cgagccacat gaccacgatg acccgtgcct acgcgatcac cgccgaggcg gtcgctgccg
    23881 agaccgaggc caacgggggc gacgtctccg cgatcgacgt ctccaaggtg gtccagctcg
    23941 acgacctgga gctgcgcgac ctcgggccgc gcgacgtcaa gctgaagatc ctcgccgtgt
    24001 cggccgagca caacatcgac cacgccgcca cggccgacac catcaacatc gccgaggccc
    24061 gcggcggcaa gatgtacccc ggcaactccg ccgtgggcga ggtgaccgag gtgggcagcg
    24121 acgtcaccgc gttcgcgccc ggcgacgtcg tcatcaccca ctgcaacggc gacccggacc
    24181 ggttcggctt cccgctgcgc atctgggcct acgacatgcc cgactccatc ggctggtact
    24241 cgaaggaggc cgtggtcgag gactggcagc tcatcaaggc gccgctcgac tccgggctga
    24301 acctctggga gatcgccgcc ctcccgctgc gggcgccgac cgcctaccac ctgtggcgcc
    24361 gcgccatcgg catctaccgg gtcaaggtcc cctacgagcg ccaggcccgc ctcaacgtgc
    24421 tcagcttcgg cggcggcgtg ggcgagctgt tcctgatgct cgccaaggcc gaggggcaca
    24481 acgcctactt ctgcgccggg tccaaggagc gccgcgacgc cctcgagaag cacggcatcg
    24541 tcggcatcga ccagaagcag ttcaaccgct tcg
//
LOCUS       DATISU010000159         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_426769, whole genome shotgun sequence.
ACCESSION   DATISU010000159 DATISU010000000
VERSION     DATISU010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426769"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2127)
                     /gene="aceE"
                     /locus_tag="VLR27_06515"
     CDS             complement(<1..2127)
                     /gene="aceE"
                     /locus_tag="VLR27_06515"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HSP03135.1"
                     /translation="MAGPMVIDGLHSDNPRHDGYVNQLPDIDPGETSEWLDSLEAVVD
                     THGKTRARFLISKLLSRANELQVGSPAAISTPYVNTIPPEREPWFPGDEHVERRIRAY
                     IRWNAAAMVVRANKKADGIGGHLATFASSAALYEVGFNHFFRGKEDGTPGDHVYFQGH
                     AAPGIYARAFLEGRLTETQLDHFRMEVGGEGIPSYPHPRLMPDFWEYPTVSMGLGPIN
                     SIYQARFLKYLTNRRVDDTSDSNVWCFIGDGETDEPETLGSISLASREGLDNLIWVVN
                     CNLQRLDGPVRGNGKIIQELEAVFRGAGWNVIKVVWGSKWDELLQRDVDGVLLNKMNT
                     TVDGQFQRYAVEDGDFIRDDFFGPDPRLRKIVEHLSDEDLRNLPRGGHDYKKLYAAYK
                     AATEANGRPTVILAKTIKGWTLGPDVEGRNATHQIKKMTKDQLRTLRDRLHLQEEVPD
                     ADLDSDLPPYISLREGTPEHEYFHSRLKALDGPLPSRPAPIRKGITMPDDKVYEELLA
                     GSGKQEAATTMGFTRLLRNLARDPEFGERVVPIVPDEARTFGMDSLFREIKIYASRGQ
                     LYEPVDHDLLLSYTEGKDGQILEEGITEAGSMASFTAAGTAHANRGVPMVPFYIFYSM
                     FGFQRVGDLIWAAADARARGFLLGATAGRTTLTGEGLQHQDGHSLLLASTVPTVEAYD
                     PAFAYEVALIMKAGLERMYGPDTPDDE"
BASE COUNT          291 a          659 c          838 g          373 t
ORIGIN      
        1 ctcgtcgtcg ggcgtgtccg gcccgtacat ccgctcgagt ccggccttca tgatgagggc
       61 gacctcgtag gcgaaggccg ggtcgtaggc ctccacggtg gggacggtgg aggcgagcag
      121 cagcgagtgg ccgtcctggt gctggaggcc ctcgccggtg agcgtggtgc gcccggcggt
      181 ggcgccgagc aggaagccac gggcccgggc gtcggcggcg gcccagatga ggtcgccgac
      241 ccgctggaag ccgaacatcg aatagaagat gtagaagggc accatcggca cgccccggtt
      301 ggcatgcgcg gtgccggcgg cggtgaagct ggccatcgag ccggcctcgg tgatgccctc
      361 ctcgaggatc tggccgtcct tgccctcggt gtaggagagc agcaggtcgt ggtcgacggg
      421 ctcgtagagc tggccgcggc tggcgtagat cttgatctcg cggaacaggc tgtccatgcc
      481 gaaggtgcgg gcctcgtcgg gcacgatcgg cacgacgcgc tcgccgaact ccgggtcgcg
      541 agccaggttg cgcaggaggc gggtgaagcc catcgtcgtc gccgcttcct gcttgcccga
      601 cccggccagc agctcctcgt agaccttgtc gtcgggcatc gtgatgccct tgcggatggg
      661 ggccggccgg gacgggagcg gcccgtcgag cgccttgagg cgggagtgga agtactcgtg
      721 ctcgggcgtg ccctcgcgca gggagatgta gggcggcagg tcggagtcga ggtcggcgtc
      781 gggcacctcc tcctgcaggt ggagccggtc gcgcagggtg cgcagctggt ccttggtcat
      841 cttcttgatc tggtgggtcg cgttgcgacc ctcgacgtcg gggccgagcg tccagccctt
      901 gatcgtcttg gccaggatga ccgtgggccg cccgttggcc tcggtggcgg ccttgtaggc
      961 ggcgtagagc ttcttgtagt cgtggccgcc gcggggcagg ttgcgcagat cctcgtcgga
     1021 gaggtgctcg acgatcttgc gcaggcgggg gtcggggccg aagaagtcgt cgcggatgaa
     1081 gtcgccgtcc tcgacggcgt agcgctggaa ctggccgtcg accgtggtgt tcatcttgtt
     1141 gagcaggacg ccgtcgacgt cgcgctggag cagctcgtcc cacttcgagc cccacacgac
     1201 cttgatgacg ttccagcccg cgccgcggaa cacggcctcg agctcctgga tgatcttgcc
     1261 gttgccgcgc acggggccgt cgaggcgctg caggttgcag ttcacgaccc agatgaggtt
     1321 gtcgagcccc tcgcgggagg cgagggagat cgagccgagc gtctcgggct cgtcggtctc
     1381 gccgtcgccg atgaagcacc acacgttgct gtcggaggtg tcgtcgacgc gccggttggt
     1441 gaggtacttg aggaaccggg cttggtagat cgagttgatc ggcccgaggc ccatcgagac
     1501 ggtcgggtac tcccagaagt ccggcatgag ccgggggtgc gggtagctgg ggatgccctc
     1561 gccaccgacc tccatgcgga agtggtcgag ctgggtctcg gtgaggcggc cctcgaggaa
     1621 cgctcgcgcg tagatgcccg gggcggcgtg gccctggaag tagacgtggt cgcccggcgt
     1681 gccgtcctcc ttgccgcgga agaagtggtt gaagcccacc tcgtacaggg cggccgagga
     1741 cgcgaacgtg gccaggtggc cgccgatccc gtcggccttc ttgttggccc gcaccaccat
     1801 ggcggcggcg ttccagcgga tgtaggcgcg gatgcggcgc tcgacgtgct cgtcgccggg
     1861 gaaccacggc tcgcgctcgg gcgggatggt gttgacgtac ggcgtggaga tggctgccgg
     1921 cgagccgacc tggagctcgt tggcccggga cagcagcttg gagatcagga agcgggcccg
     1981 ggtcttgccg tgggtgtcga cgacggcttc gaggctgtcc agccactcgc tggtctcgcc
     2041 ggggtcgatg tcgggcagct ggttgacgta cccgtcgtgg cgggggttgt cggagtggag
     2101 gccgtcgatc accatgggcc cagccaacca cgagatcgtg ccgtttgctc ccccgggtag
     2161 g
//
LOCUS       DATISU010000160         5598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_53922, whole genome shotgun sequence.
ACCESSION   DATISU010000160 DATISU010000000
VERSION     DATISU010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53922"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VLR27_06520"
     CDS             <1..488
                     /locus_tag="VLR27_06520"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HSP03136.1"
                     /translation="IAELRAATGEFQVVIDVEDKTRFVPGFVKGERTTFLAQGAVDAL
                     VDLGELDDDSIIIAEDGSVTVVLPPAKLADPVIDHEASGVLDRDRGVMDRVGGIFSDE
                     PTSDKDLYQEAERRLTEAAADSELRTTGEAKAAATVEQLLREAGIEQVRVIFAEPEAV
                     A"
     gene            553..1257
                     /locus_tag="VLR27_06525"
     CDS             553..1257
                     /locus_tag="VLR27_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HSP03137.1"
                     /translation="MTTHDDRIADALVAFIGTLTQVHTVEAVFQHLGRYCTELLPAEG
                     VGVLLLEEQSLTVATANSEVGDAVEALEVELQEGPCVECVRVGHPVLVPDLAAAADRY
                     PRFVPRALEAGAGAIHALPMTGQGELVGSLDIISLQPTELTESQLSTARMLADVAVSY
                     IFAVRLHEETSELAGQLQNALDTRVVIEQAKGMLAERHGESLGDAFNRMRALARSRST
                     PVREIARQVVDDRLQP"
     gene            complement(1319..1531)
                     /locus_tag="VLR27_06530"
     CDS             complement(1319..1531)
                     /locus_tag="VLR27_06530"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HSP03138.1"
                     /translation="MSGKTYKKTNLIGESPDSIEAAVETALKRSAQKVRGQEWCEIKD
                     IRANVNESGGVDLWQVELQVAFVVES"
     gene            1699..2127
                     /locus_tag="VLR27_06535"
     CDS             1699..2127
                     /locus_tag="VLR27_06535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP03139.1"
                     /translation="MPRPPQRQEDFSGSYGPDPAAIHDARQDFSRWLDASLDDGDILD
                     EMLVVLSELMANAVDATQDPTGDVTVKAWVDADGLTLKVTNPPASTFAAVNRWDYDDP
                     LRAGGRGLLIVESLVDDLAIAPPNGAEPLSVRARRDLRSD"
     gene            2400..3134
                     /locus_tag="VLR27_06540"
     CDS             2400..3134
                     /locus_tag="VLR27_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HSP03140.1"
                     /translation="MTDPSAGSRERAILDAFIDLADSLVADYDPLDFLYRLLDHSIPL
                     TEADAGAVLLHYEGRLHLVASSDEDAQAVELFEVQNEQGPARDAFHGGELVRVPRMSD
                     ATDRWPEFVEAAAPYGWSSVFAVPLRLRDHTVGSFVAFWPGKMLNEQDAKVLRGFADV
                     AAIAVLQQRATADVEMVNKQLHTALETRINIEQAKGMIAQATGVRMGEAFEMLRRHAR
                     SKSTRVTNAAASVVAGDVDIHVLTRH"
     gene            complement(3184..3804)
                     /locus_tag="VLR27_06545"
     CDS             complement(3184..3804)
                     /locus_tag="VLR27_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03141.1"
                     /translation="MSNPTSDPRSTTTRDRDDIEIEHDRIGVHNEAQTVRRDRIRWGP
                     VWAGVVTAVGSYLFLMLALVALGIVDMGDGVTGDAIASGIAALVAFFIGGVTTGASSM
                     WQGADDGVLHGIVMWFAALVALVVIGAVGSGVALGALDTSDAFDEFSADEVDTDQANE
                     DAEEAAAKALFGLALALGASAAGGAAGAKMWPKDDAFIERTRVTRS"
     gene            4008..4898
                     /locus_tag="VLR27_06550"
     CDS             4008..4898
                     /locus_tag="VLR27_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP03142.1"
                     /translation="MPIIDLAARRHRRVERDSHDPRVPTGQQITLPDGAETFVRVGGR
                     PDGAPTLLLHGLGATAALNWAQCFPELEQLGPVIAPDHRGHGRGARIGRHFRLEQCAD
                     DAAAILLALDAGPAMVVGYSMGGPIAQLLARRHPELVSGLILSATARDFRGAPADRLR
                     YAALAAIAPAAHLIPPFGLLPAPSLPGPLRRLSSATSEIRRHEAPAVLAAARELGRFT
                     SRSWVHHLTVPTAVLVHTDDGVVPPRRQHKLAAAMPDAHTIEVDGDHLAITNDPDRYI
                     PALLEAHESVTRRTVTATAA"
     gene            4973..>5598
                     /locus_tag="VLR27_06555"
     CDS             4973..>5598
                     /locus_tag="VLR27_06555"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HSP03143.1"
                     /translation="MPSKPRTSQLSAQGWRSSIWVWPAVTGFTSFVLAQVLTTLEPAE
                     GSRFTALWQGGGTSAINVLGVVATSVMTATTLIFSLTVLALQLASQQFSPRLLRQFAR
                     DRVTKRVLSILAGSFIFSLSAMSGVSADGPVPTLALAVALLLGVASFAAILAYITHMV
                     RILRVDTMMLHVHDDTDEAIRTFYPSSEEPHRDPEELDLDVGDAHWVSA"
BASE COUNT          822 a         2100 c         1825 g          851 t
ORIGIN      
        1 agatcgccga gctgcgggct gcgaccggcg agttccaggt cgtgatcgac gtggaggaca
       61 agacccgctt cgtgccgggc ttcgtgaagg gcgagcgcac cacgttcctc gctcagggcg
      121 ccgtcgatgc cctggtggac ctgggcgagc tggacgacga ctcgatcatc atcgccgagg
      181 acggctcggt caccgtggtg ctcccgccgg ccaagctcgc cgatccggtg atcgaccacg
      241 aggcatccgg cgtgctcgac cgggaccggg gcgtcatgga ccgggtgggc ggcatcttca
      301 gcgacgagcc cacctcggac aaggacctct accaggaggc cgagcgacgc ctgaccgaag
      361 cagctgccga cagcgagctg cggaccacgg gcgaggccaa ggccgctgcc accgtggagc
      421 agctcctccg cgaagccggg atcgagcagg tccgggtgat cttcgccgag cccgaggccg
      481 tggcctgacc gaaggtccac tgccgacagc gccggctcgg gtgcctagtc tcccggtcga
      541 ggtgaatgca ccatgaccac gcacgacgac cggatcgccg acgccctggt ggcgttcatc
      601 ggcacgctga cccaggtcca caccgttgag gccgtcttcc agcacctggg gcgctactgc
      661 accgagctgc tccctgcgga gggcgtcggc gtgctgctcc tcgaggagca gagcctcacg
      721 gtggcgacgg cgaacagcga ggtcggcgac gcggtcgagg ccctcgaggt cgagctgcag
      781 gaggggccct gcgtcgagtg cgtacgggtc ggccacccgg tgctcgtccc cgatctggcc
      841 gcagccgctg accgctaccc gcggttcgtg cctcgggcgc tcgaggcggg tgccggtgca
      901 atccacgcgc tgccgatgac cgggcagggc gagctggtcg ggtcgctcga catcatcagc
      961 ctgcagccca ccgagctgac agagtcccag ctcagcacgg cgcggatgct ggccgacgtc
     1021 gcggtgtcct acatcttcgc ggtgcggctc cacgaggaga cgtccgagct ggctggccag
     1081 ctccagaacg ccctcgacac ccgcgtggtc atcgagcagg ccaagggcat gctcgccgag
     1141 cgccacgggg agtcactggg agacgcgttc aaccggatgc gcgccctcgc ccgcagccgg
     1201 agcacaccgg tgcgcgagat cgcacggcag gtcgtggacg accgtctcca gccctgacgg
     1261 gcagaccgcc agggctggtt cgatcggcgg tggcgacccc gggcggggtc gccgctcgct
     1321 acgactcgac gacgaaggcg acctggagct cgacctgcca gaggtcgacg ccgccgctct
     1381 cgttcacgtt ggcccggatg tccttgatct cgcaccactc ctggccgcgc accttctggg
     1441 cggagcgctt gagggcggtc tcgacagccg cttcgatgct gtcgggggac tcgccgatca
     1501 ggttcgtctt cttgtaggtc ttgccggaca tggggtctcc tcgttcgggt tcgttgggcg
     1561 ccccgtcccc tacccgtcgt cggtgcgcat caccacgtcc ggcgggcgct cggcgccggt
     1621 ccggttcctg ggtggcgctg cgacgtcgtg ggtatgggtc tcggcaccac cgaccagcgc
     1681 acccatcccg gagcccgcat gcccagaccc ccgcagcggc aagaggactt ctcgggctcc
     1741 tacggcccgg atcccgccgc catccacgac gctcgccagg acttctcgcg ctggctcgat
     1801 gcctcgctcg acgacggcga cattctcgac gagatgctgg tggtcctgag cgagctgatg
     1861 gccaacgccg tcgacgcaac ccaggatccc acaggcgacg tcacggtgaa ggcgtgggtg
     1921 gatgccgacg ggctcaccct caaggtcacc aacccgcccg cctcgacctt cgccgcagtg
     1981 aaccggtggg actacgacga cccgttgcga gccggtgggc gcggcctgct catcgtcgag
     2041 tccctcgtcg acgacctcgc catcgctccc ccgaacggtg ccgagccgct cagcgtccgc
     2101 gcccgccgcg acctgcgttc ggactgaccg ggcgcagcca acggttccgc agacgaaccg
     2161 ggaagccgtg cacgcatccg gtgcgtgctc cgcggcccgg aacgggaggg cgcgatcgtg
     2221 gcgacgttgt gcccggtgtg tcggcgatgg gcagcggggt ccgggccgtc ccaccgaccc
     2281 ggtgggcacg gtatcgggat ctccggggtt gtcttcgtgt cgcccagctt cctgtcgctg
     2341 ctttggagag gttctcctca gctgcgggaa cgggaaggca catcgttgtg tccgattccg
     2401 tgaccgaccc ttccgccggc agccgggagc gcgccatcct cgacgcgttc atcgatctgg
     2461 cggactcact cgtcgcggac tacgacccgc tcgacttcct ctaccgcctg ctcgatcaca
     2521 gcatcccctt gaccgaagcc gatgcgggtg cggtgctgct ccactacgag ggtcgcctcc
     2581 acctggtcgc atcgtcggac gaggatgccc aggccgtcga gctgttcgag gtgcagaacg
     2641 agcaaggccc agcacgagac gcgttccacg gcggcgagct cgtccgagtt ccccgcatga
     2701 gcgacgcgac cgaccgctgg cccgagttcg tcgaggcggc agccccgtac ggctggtcgt
     2761 ctgtgttcgc cgttccgctg cgcctgcgtg accacaccgt ggggtccttc gtcgccttct
     2821 ggcccggcaa gatgctgaac gagcaggacg ccaaggtcct gcggggcttc gccgacgtgg
     2881 ccgccatcgc cgtcctccag cagcgggcca ccgctgacgt cgagatggtg aacaagcagc
     2941 tgcacaccgc gctcgagacc cgcatcaaca tcgagcaggc caagggcatg atcgcccagg
     3001 ccacgggcgt ccgcatgggc gaggcgttcg agatgctccg ccgccacgcc cggtcgaaga
     3061 gcacgcgagt caccaatgcc gccgcgtccg tggtcgccgg ggacgtagac atccacgtcc
     3121 tgacccgcca ctgacgaacc accgctgccg ggccgtaggc ccgacagcgg tggtcggccc
     3181 ggttcagctc cgggtaacgc gggtgcgctc gatgaaggcg tcgtccttgg gccacatctt
     3241 ggctccggcg gcgccaccgg ctgcggaggc accgagggcg agcgcgaggc cgaagagggc
     3301 cttggcggcg gcttcttcgg cgtcctcgtt ggcttggtcg gtgtcgacct cgtcagcgga
     3361 gaactcatcg aaggcatcac tggtgtcgag cgcgccgagg gcgacgccgc tgccgacggc
     3421 gccgatcacg accagggcca cgagggcggc gaaccacatg acgatgccgt ggagcacgcc
     3481 gtcgtcagcg ccctgccaca tcgaggatgc tccggtggtc acgccaccga tgaagaaggc
     3541 caccagggca gcgatcccgg atgcgatcgc gtcgccggtc acgccatcgc ccatgtccac
     3601 gatgccgagg gcgacgagag cgagcatgag gaacaggtac gagccaacgg cggtgaccac
     3661 gccggcccac accgggcccc agcggatccg gtcccggcgg accgtctgag cttcgttgtg
     3721 cacgccgatc cggtcgtgtt cgatctcgat gtcgtcgcgg tcccgcgtgg tggtgctgcg
     3781 cgggtcagaa gtcgggttgc tcatggcagt ccccttgtgg ttcggcgccg gcaggagccg
     3841 gcgcatcggc gcgtcactac cccggcgggt gatgcttcac gccatgtggc gttgctcgtg
     3901 ccgtcatcgc ctcaagccgc gacgactcct cgctccttgc caggtgatgt gcccgatgcg
     3961 acacgggact cgtgcttggc gcatcacccc cggggtagcg atgcgccgtg cccatcatcg
     4021 acctcgccgc ccgtcgccac cgacgggtcg aacgcgacag ccacgatccc cgagtcccca
     4081 ccggccagca gatcaccttg cccgacggcg ccgagacctt cgtccgcgtc ggcggccgtc
     4141 ccgacggagc gccgacgctc ctgctccacg gcctcggtgc cacggcggcg ctcaactggg
     4201 cccagtgctt ccccgagctc gaacagctcg ggcccgtgat cgcccccgac caccggggcc
     4261 acggccgcgg cgcccgcatc ggtcggcact tccgcctcga gcagtgcgcc gacgacgccg
     4321 ccgccatcct cctcgcactc gacgccggac ccgccatggt cgttggctat tccatgggcg
     4381 gcccgatcgc ccagctcctc gcccgacgcc accccgagct cgtctccggt ctcatcctga
     4441 gcgcaacagc tcgcgacttc cgcggtgcac ctgccgaccg gctccgctac gccgcgctcg
     4501 ccgccatcgc ccccgccgcc cacctcatcc caccgttcgg cttactgccc gcgccgagcc
     4561 ttcccggacc gcttcgccga ctcagctccg cgaccagcga gatccgacga cacgaggccc
     4621 ccgcggtcct ggccgccgca cgagagctcg gccgcttcac cagccgcagc tgggtccacc
     4681 acctgaccgt ccccactgcc gtgctcgtcc acaccgacga cggcgtcgtc ccaccccgcc
     4741 gccagcacaa gctcgctgcc gccatgcccg acgcgcacac catcgaggtc gatggtgacc
     4801 acctcgccat caccaacgac cccgaccgct acatccccgc cctgctcgag gcccacgagt
     4861 ccgtcacccg acgcacggtc accgcaaccg cggcctgacg acgtcgccga cccgagcgca
     4921 gctcatcccc ttcaggtaac tagcccgccg acccgtcggg tagtgctacc ccgtgcccag
     4981 caaaccccgc acgagtcagc tgagcgcgca gggctggcgg tcctcgatct gggtctggcc
     5041 cgccgtcacc gggttcacct ccttcgtgct ggcccaggtg ctcaccaccc tcgagcccgc
     5101 cgaaggctcg aggttcactg cgctctggca gggcgggggc acctcggcga tcaacgtgct
     5161 gggcgtcgtc gcgacctcgg tcatgacggc aaccacgctg atcttcagcc tcacggtcct
     5221 cgcactgcag ctggcatccc agcagttctc gccccgcctg ctccgccagt tcgcccgcga
     5281 ccgcgtcacg aagcgtgtgc tgtccatcct cgcaggcagc ttcatcttct cgctcagcgc
     5341 catgtcgggc gtgagcgccg acgggccggt gcccacactg gccctcgcag tggcgcttct
     5401 gctcggcgtc gcatccttcg ctgcgatcct cgcctacatc acccacatgg tccggatcct
     5461 gcgcgtcgac acgatgatgc tccacgtcca cgacgacacc gacgaagcga tccgcacctt
     5521 ctacccgtca tccgaggagc cccaccggga cccggaggaa ctcgacctcg acgtcggtga
     5581 cgcccactgg gtgtccgc
//
LOCUS       DATISU010000161        28546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_705992, whole genome shotgun sequence.
ACCESSION   DATISU010000161 DATISU010000000
VERSION     DATISU010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 28546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28546
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705992"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..675
                     /locus_tag="VLR27_06560"
     CDS             193..675
                     /locus_tag="VLR27_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HSP03144.1"
                     /translation="MAEVTETQLPGVGVRYEFRTGDRDHVGVVHHHSGRREIVVYDRK
                     DPDVARSVLELSADDSRTLSDLLGASQVTEALAAVQQRIEGLTLEWIEVTEDSSMAHR
                     SIGDGQLRTRTGASVVAVIRGDTSEPAPSPDYVLEPEDVVVAVGTADGLELLRQALRP
                     "
     gene            672..1868
                     /locus_tag="VLR27_06565"
     CDS             672..1868
                     /locus_tag="VLR27_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSP03145.1"
                     /translation="MTPVLAAVSGETAEAFIEIGGVVVVLAVLARIAGRLGITAIPLY
                     LLAGLAVGEGGFTPLEAGQDFLAISGQIGVLLLLFALGLEYTADELRHGLRTGTGTGA
                     VDAVANFLPGFLAGLALGWETTSAVLLGGVTWVSSSGVVAKVLTDLHRLGNRETPSIL
                     NVLVIEDLAMAVYLPIAAALVAGRTALDTAVTVGIALAAVGVILVLALGSGARLSAML
                     APGGDESLLLSVFGLTLLVGGLAEQLQVSSAIGAFLVGLALSGAVQQRATILVEPLRN
                     LFAPIFFLYFSFGIAPDSLVEVLLPALVLAVVSGATKMLSGWLAAARAGVGVRGRMRA
                     GTALIARGEFSIVIASLGVGAVDGADLGAFAAAYVLITAVLGPLAAKYADQIPIPARL
                     IRPAPA"
     gene            complement(1875..2303)
                     /locus_tag="VLR27_06570"
     CDS             complement(1875..2303)
                     /locus_tag="VLR27_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSP03146.1"
                     /translation="MDSTRGTSLDHQGLEVIAPEECWQLLADSPVGRVAFNEAGEPMV
                     LPVNHAVVGHRVVFRTLRGSLLHEALMAEPVAFQVDGFDVIERTGWSVLVRGVADLAE
                     DPEELEKLELHPWADSVEREDWVQIRAEEITGRRIVSSGS"
     gene            complement(2361..2867)
                     /locus_tag="VLR27_06575"
     CDS             complement(2361..2867)
                     /locus_tag="VLR27_06575"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HSP03147.1"
                     /translation="MTAAPQTLVLTGVSGAGKSTVGRALADRIGAVLLDADDFHPEIN
                     RQKMAAGVALTDADRDPWIDTLAAELERRSRDGDRVVLACSALRRHHRARLLDAAPDV
                     ALVHLDVGPEELARRLAGRTEHFMPADLLDSQLADLEHPSGEHHAVVDGEAPVDAVVD
                     DILRVAAE"
     gene            complement(2895..3938)
                     /locus_tag="VLR27_06580"
     CDS             complement(2895..3938)
                     /locus_tag="VLR27_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HSP03148.1"
                     /translation="MKALQLTAFKQPGELVEVDQPEPGPGEVLLRIGGAGACHSDLHL
                     MHDFEPGMMPWNPPFTLGHENAGWVEALGAGVSGLEVGEAVAVYGPWGCGLCRRCALG
                     MENYCERPDLTGGAVGGLGADGGMAPYMVVPSVRHVVPIGDLDPAEAAPLTDAGLTPY
                     HAVKRALPLLTPGTTAVVIGVGGLGHMAVQMLRALTPATVIAVDQRDDALRLAESVGA
                     HHGIVSDDEAAATIRDLSGGQGADVVLDCVGADATLALGAAASRVLGQLTIVGIAGGT
                     LPMSFFSIPYEVSVATTYWGSLPELHEVLAMARSGIITPHVTRFDLDEAPSVYERLAA
                     GEIEGRAVIVPTS"
     gene            complement(4020..5159)
                     /locus_tag="VLR27_06585"
     CDS             complement(4020..5159)
                     /locus_tag="VLR27_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSP03149.1"
                     /translation="MARPDPGPDQVQPAGPPRVPRWLDVLTAWSWRLLIVGLAVYATG
                     IVASQLRIVLLPVGIALLLSTVLIPVRKLFTDRGVPVPLAMLATVAVFFGTVFFVGWV
                     IVPPLIDEFSGLDATLETAADDVQEWFIDGPLGLSEDFVLDARERIEEAADSASVSEG
                     AVVDGATLAGEVLAGLLLALVVTFFVVKDGPMMQRALLSRFSDSRQVQLRAAGNGAWR
                     ALGGYLRGAAALGVIEGIVIGITMIIVGANLALPVAALTFVSAFFPFVGAIAAGIIAV
                     SVTLVTAGSAAAGVVAIVAILVQQLDNDLLAPVIYGKALQLHPLVVILALTTGAAIAG
                     IAGAFIAVPLVAVALRGAAAVRALQEGDDEDPDAARALDSSADDP"
     gene            complement(5182..5838)
                     /locus_tag="VLR27_06590"
     CDS             complement(5182..5838)
                     /locus_tag="VLR27_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HSP03150.1"
                     /translation="MVFSFFRGGDEGLEHVQHEILSMVGRCQHSFDLAMSCLVTDGDI
                     EPIGEEVRATDWAINGIEESVRRELVVHSAVHGGADVGAVLASLLMVKKLERVGDQAK
                     NIFDLAAEGVRFSEADDYERFLDFRSRVSQLYTDTADALAEPDTADVEGLGRRAEELM
                     GTFDGLVNALIHADEPARYAVPRAMLFRYLKRICANLTSVATTAATGIDRTGDVDLDE
                     "
     gene            complement(5850..7004)
                     /locus_tag="VLR27_06595"
     CDS             complement(5850..7004)
                     /locus_tag="VLR27_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HSP03151.1"
                     /translation="MVSSRPSTTDAATERPVPAPIRAGVVIALLFLFLVGVGLLESGI
                     NALGSDVQEELFSSVSNPMAGLFVGILATVLAQSSSVTTSTIVGLVGAGLLGIDDAVP
                     MIMGANIGTTVTNTLASLGHLRRPAEFRLAFAAATVHDAFNLLAVSVLLPIELLTGVL
                     SSAAEFLTELLLGGSGAEFDSPFKAAVKVPVGWVEELWGDVVGLQGSLLGVVLVVTGL
                     AAIFLALTFVTRNMRQLIAARVERSINAMLGRGGGVPAILLGVIITVAVQSSSITTSI
                     MIPLSAAGVVSLVNAYPVTLGANVGTTISALLASLATDRPEALTVALVHTLFNVAGIL
                     LFYPVPALRRLPIRIAEALAEMAARRRSMVIGYVIVTFLVIPAIGVILLR"
     gene            complement(7129..7974)
                     /locus_tag="VLR27_06600"
     CDS             complement(7129..7974)
                     /locus_tag="VLR27_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP03152.1"
                     /translation="MSQTTITEDPTTDSRIGWRDTLSDIGVIAHRNVIKITRVPEVLV
                     FVLLSPIMFVLLFAYVFGNSIDIEGTSYREFLIAGIFAQTVLFGATFTGAGIAEDMQK
                     GIIDRFKSLPMSRSAVLAGRTASDVIYNVLSVAIMALTGLAVGWRIRGSILEAVAGFA
                     LLLLFAYAISWVMAWVALLVPSVEVINNASFMVIFPLTFVANTFVPAEDLPGPLQTFA
                     EWNPVSAVTQAARELFGNIPAGTAEPTAWSLQNPVLYTLIWVVITVAVFAPLSVSRYQ
                     RTSKR"
     gene            complement(7971..8978)
                     /locus_tag="VLR27_06605"
     CDS             complement(7971..8978)
                     /locus_tag="VLR27_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSP03153.1"
                     /translation="MHQSTSHSAAIVATGLRKRFGDVVALDGLDLTVPEGSVLGLLGP
                     NGAGKTTAVSILTTLLEPDEGSATIAGADLRTQPGEVRRRIGLSGQFAAVDEVLTGRE
                     NLFMIGRLYRLGRTRSKQRAAELLERFDLTDAGDRPVKTYSGGMRRRLDLAGALVAEP
                     PVLFLDEPTTGLDPRSRIELWGVIRELVSRGATLLLTTQYLEEADQLADDIVVIDHGR
                     AIASGTADQLKAQVGGERIELVIADPARLDEAHQVVAAHAAGDIEVQVPARSLQAPID
                     GGVDVLTDVLQELRHRQIEISDVGIRRPTLDDVFLTLTGHVAEEADDASDRTEREGVT
                     A"
     gene            complement(9115..10092)
                     /locus_tag="VLR27_06610"
     CDS             complement(9115..10092)
                     /locus_tag="VLR27_06610"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSP03154.1"
                     /translation="MQYVKLGRTGLDVSRICLGCMSFGAPGAGNHEWSLDEEASRPFI
                     RQAVEHGINFFDTANVYSAGTSEEIVGRALQEYANRDDVVLASKVFGPMRKGPNSAGL
                     SRKAIIAECEASLRRLGTDYIDLYQIHRWDPRTPIEETLEALHDLVRSGKVRYIGASS
                     MWAWQFSKALYTAELRGWTRFVSMQDHYNLLNREEEREMLPLCEDQGIGVIPWSPLAR
                     GRLTRDWDTETDRSRTDEFGKTLYDTSASDREIVEQVAAVAEARGISRAQVALAWVLH
                     QPAVTAPIVGATKPHHLDDAVAAVDVTLTAEEVESLEAPYTPHPVVGFA"
     gene            complement(10142..11320)
                     /locus_tag="VLR27_06615"
     CDS             complement(10142..11320)
                     /locus_tag="VLR27_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSP03155.1"
                     /translation="MAVGDATVRLHEAIDLVAGIDVSEVPTAEGLGELVEELTRCLRR
                     LDFELHRLLRSFEARGDHVPLGYRTAKQWCRDRLRMGDAAAATSLKLGRVLIDMPGVS
                     ARWAAGDLGAEHVTAIAAVRTEETREAFGEWEGWLAQQAVDLDMRGLRKVLAKWRLQV
                     DPDSGEPERIERDRDAHVSATFQGAVVIDAVLDAIGGAAFKTVFDGIVEELFHADWHE
                     AKTTLGREPLSSELARTPAQRRADALVVMAQRAHTANPSAAARLRPLVVVVAGLDALA
                     GGLAELWNETPLSARQLARILQDDPDLERYVYGPGEIPIAYNERHRFFTGKLRRAIQV
                     RDRHCTARGCDRPAEYCDIDHIDPVTQGGGTRPDNGRVACNPDNRARPQRRFHGDPDP
                     "
     gene            11432..12634
                     /locus_tag="VLR27_06620"
     CDS             11432..12634
                     /locus_tag="VLR27_06620"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP03156.1"
                     /translation="MGTLGSLGPGFRALYRGALVSNVGDGIRLAALPLLATSLTTSPF
                     LVAAVTAAQYLAWLAFGPFGGALVDRADRRRTVLTTQLWRGLLMAGLGLLVWSGHAAI
                     WHVCVVAFAITIGEILVDPSTVALVPTLVDDVDLERANGRIAGVEIVTNDFAGAPVGA
                     ALYALAPWLPFVVDACSYLGSLLPFRRLPRRTRTAPSSNPAPRLRTDAADGFRWLRRH
                     RVLAPFTVSQVIYYFGFSAGFSLLVVLVTEELDGSAVAFGVVLAIGAGGAFLGSLAGA
                     RVSSAIGTRATLSGCVALQGATLAAASIAPSLPAVAALWLANGIPAGLQRPVARSMQQ
                     RLTPNHLLGRVNVTTRIFTRGIIVFGALAAGALASTTDIRWSFAVGGAIQVVAAVAMW
                     RVLARLRA"
     gene            complement(12644..13390)
                     /locus_tag="VLR27_06625"
     CDS             complement(12644..13390)
                     /locus_tag="VLR27_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03157.1"
                     /translation="MKAGNLIRSELRKLTTTSMPLAFVAVLIVLAGINAVAVALGTDA
                     DGSKTFISTADDQRSLVAFAANATLLAGLFGAIAAAREHGHGTIIHSFLAAPRRHRAV
                     LAQFAGVGLGGALLGLLGAGTTILAVALALPTTEFGFLLSIGGVARVLAASTLCGAAG
                     AVLGAGIGALVRNTGGAVTGAVLVLLVAPPLVVQLASGTASWMPATLATVLSGASAEV
                     GALAAAVAMCLWAAVPAAVALLVVQRRDVL"
     gene            complement(13387..14325)
                     /locus_tag="VLR27_06630"
     CDS             complement(13387..14325)
                     /locus_tag="VLR27_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP03158.1"
                     /translation="MTGEPHEGVAIEVEGLTKRYGDATVVDDLTFSVRPGRVTGFLGP
                     NGAGKSTTMQMVLGLAAPTDGRATIGGRRYADLDDPVRTVGALLEADAFHPGRTGRNH
                     LRVLSDAAGIPGTRVDEVLETVELTAAADKRVGAYSLGMRQRLGVAGALLGDPPVLVL
                     DEPANGLDPQGIHTMRDLLRGCAERGSTVLVSSHLLAEMELLADDLVVIAAGRLVAHS
                     SLADLQQTEIVVRTPSADVLAAALRRCGPSVEVTQPPGDPDRLLVRGLSIDAVGGCAF
                     EAGVVLHELSTHAGSLEERFLAWTSTPATDGGSEAA"
     gene            14514..14963
                     /locus_tag="VLR27_06635"
     CDS             14514..14963
                     /locus_tag="VLR27_06635"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSP03159.1"
                     /translation="MAKNCVRTDLSPDEVFEHLLDPWHYPDWLLGASTMRDVDDSWPA
                     VGSAFHHRVGFGPIKINDRSKVLEIDAPKRLVLHVRATPAVQAVVTFTVEPTAEGSVL
                     WLEEQPAMKIGQLLRPLLDPPTHIRNKASLENLADLMQRDREADAAE"
     gene            complement(14975..15781)
                     /locus_tag="VLR27_06640"
     CDS             complement(14975..15781)
                     /locus_tag="VLR27_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSP03160.1"
                     /translation="MADDALTDFERTTFEHAGTTRDVFRLGTGPAVVVIAEMPGITPK
                     VADFARRVAGIGCTAVLPHLFGEPGRDPAPDGKPDLRYMATSMVPACVSKEFTVWATG
                     RTSPIIGWLRALAEAEHERCGGPGVGAIGMCFTGGFALAMATDDRLLAPVLSQPSMPV
                     ALTRSRKGAIDISPEDLAVVKQRCADGLEVLGLRFRSDRLVPSERFRFLERELGDAFR
                     AVELDDDAANPDSKMSPHSVVTEHLVDEPGEPTRQALDDVLELFRSRLLG"
     gene            complement(15810..16052)
                     /locus_tag="VLR27_06645"
     CDS             complement(15810..16052)
                     /locus_tag="VLR27_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03161.1"
                     /translation="MTIALTIAAVVLLTAVMLLVARAGGRKHQEDLSGHGSSVAGSDE
                     HGRPAQPKSDRPAGPDAEAMGVSDAGQPSVDPTSER"
     gene            complement(16096..17619)
                     /locus_tag="VLR27_06650"
     CDS             complement(16096..17619)
                     /locus_tag="VLR27_06650"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HSP03162.1"
                     /translation="MAATTPPPPPPRAPGNGRAMGAGRVVLVVAGSLAALIGFALLVS
                     GAVLGWAHATQRDDAGYYATGSHPIESAGHAVTSAHIDLGTDVREGDWVPFDDLGTVR
                     LEVAAPDGGDLFVGIGPSAEVDGYLDDVAHHRIDDLTTRPFEVEYRSMPGDATPAPPA
                     DQGFWAATATGEDLTWDVEDGDWTVVVMNPDGSAGVRADVAVGVKTDLLAPIAIGLVI
                     AGLLALGVAAVLLIVGLRRAATPPSASAAPAAAPVAGAPVSEVPDPARAYPARLDATL
                     DEPLSRWLWLVKVVLVIPHVVALVFLWMAAFVLTVVAGVAILFTGRYPRPLFDFNVGV
                     MRWTWRVGFYAFAAFGTDRYPPFRLGVEPGYPADFEVDHPERLSRGLVLVKWWLLALP
                     HLVIVSIFSGGWSAGADDDWRVVGGGGLIGVVALIGAVVLAFRGRYPRELFHFVMGLN
                     RWCFRVLAYVALMRDEYPPFRFDGGGTDPGTRPVDPAPPGPPPPQEATTREPVEIPG"
     gene            complement(17695..18048)
                     /locus_tag="VLR27_06655"
     CDS             complement(17695..18048)
                     /locus_tag="VLR27_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03163.1"
                     /translation="MSQHDAARLDLRTMLPLAGDGVHWTLEPDADLNVNLVHLDAGHE
                     VGAHVNDAVDVLVVGLDGTGEAEVDDRLHDLAPSVALHVAKGTRRRIRAGGLGFTYLT
                     VHRRRSGPAISPRRP"
     gene            complement(18045..19304)
                     /locus_tag="VLR27_06660"
     CDS             complement(18045..19304)
                     /locus_tag="VLR27_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03164.1"
                     /translation="MPGPRRVDRPRTHRYFPLTTRTIPPDRTAHRVALTQRQARPTLL
                     AAAAFAVAAPVAAVVPHDTGSWLPLHLLLVGALLTAISGATQLLAVTWSSSPAPGDRI
                     VAAQRWSVVLGAVGVASGREVDLDAVTGTGALLLGVGLVLLGVILVGIRRRSSVDRFH
                     PAIEAYVVAAAFGVAGIAMGAAIAAGDAGEWWVRLRAAHLAVNVLGLVGLVVAGTLPY
                     FVATQARMKMSRRASPARVRASTTVLTLATLVAAGGHLLDRPGIAGVGLLGYALGLVA
                     VAATLPVPGRRQLRWAGPRLVQLGAGAVWWLAMVVALAVVRLTDATTEGRVLAVLAVG
                     GFAQILVASLAYFGPVLRAGGHQRLTAGFATTASWPGLVLANVAAVALLVDARPVAVG
                     AAALWAIDTGWRVARLLTDDGGVEQPG"
     gene            19320..19979
                     /locus_tag="VLR27_06665"
     CDS             19320..19979
                     /locus_tag="VLR27_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP03165.1"
                     /translation="MNTLQEQAKALGDPTRHAIFRMVAASPEPVDVAALTEHFGLNHN
                     AIRQHLAKLVAADLVLESRAATGRPGRPRLTYVVDAAADSRWGVVGPYERLSQLLAEV
                     IRTGDSPVEVGRRAGRREAAARQLDGDAEALVGAVCETIARQGFDPDLHRTGDAVTVE
                     LRACPFMTTATTDPDTVCAMHLGIAQGMTEDADGLVVDELVARDPRRETCELRMHIEP
                     R"
     gene            complement(19985..21232)
                     /locus_tag="VLR27_06670"
     CDS             complement(19985..21232)
                     /locus_tag="VLR27_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP03166.1"
                     /translation="MNVQPIPRASDRINEVRALTAQIVNREILPNENMIWAESRGTTL
                     NDEEREVAKKTREHIKDVVKQAGLWAPHLPAEYGGADLDFLELAYMYEVLSYATGAAA
                     LFGVVAPNSGNQKILLKYGTDEQKQKWLMPLIEGTMESGFSMTEPDQPGSDPRSIKTT
                     ARRDGDEWVINGHKWFTSNGLRADFYIVMCRTEDAEGGADRNASMTQIIVPTDTPGVE
                     IVRGINVWGRPSDHCEIIYDDVRVPVDNALGERGSGHQAAQDRLGAGRVYHCMNSIGA
                     MWRAFDLMVERTTEREVHGGLLETKQMVQGMIADSYMDLQSARLMTIDCAEKMAAGQD
                     ARTDISAIKVFVPAAFHRVVDRAIQVWGAAGVTGDLPLSAMYTGARTLRLADGPDEVH
                     RILIAKNILGQYRGSEKGWDFGN"
     gene            21335..22606
                     /locus_tag="VLR27_06675"
     CDS             21335..22606
                     /locus_tag="VLR27_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HSP03167.1"
                     /translation="MQDQATVLERLDELVPTMIEDVRDLVRIPSVGGTDAENEAQAAM
                     ARRFERGGLDVDHWRIDLDAITSHPDFPGVEVDRREAWGLVGRLPGSGDGPTLLFDGH
                     VDVVPTGDRDAWAEDPFAGALRDGRIHGRGSADMKAGLLAAHWAVQAVRAAGVELRGD
                     VLLAPVQGEEDGGLGTFALLQRGWTADACVIPEPTDLDVIPANAGALTFRLRIRGRAT
                     HAARRAEGVSAIDKLVPVLGALADLERRRHEQVDPLAARWELAHPLSLGTVRAGDWAS
                     SVPDLLVAEGRIGVAIGEPVEDTRAALEAAIASACAGDPWLRDHPVEVEWWGGQFASG
                     RIPAGSDLVERVQRAHRAGGGHPEQEVYGATYGSDLRLLTELGGIPTVQYGPGDVQLA
                     HGPHESVPVDEVVTAARAFALLVLDVCGPAE"
     gene            22636..23646
                     /locus_tag="VLR27_06680"
     CDS             22636..23646
                     /locus_tag="VLR27_06680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSP03168.1"
                     /translation="MTRAPLSILDLALIGRDQTPHDALAGTVALAQRAEEHGYRRVWY
                     AEHHNMSTIASSATSVLIAHVAAHTRSIRLGAGGIMLPNHSPLTIAEQFGTLESLHPG
                     RIDLGLGRAPGSDQNTMRALRRDPRSSDSFPQDVLELQGYLRDETRIPGVNAIPGRGT
                     DVPLYILGSSLFGAQLAAMLGLPYAFASHFAPQALEQAVALYRTEFRPSEQLQEPYVI
                     AGVNVIAADSEEDAAEQHLRVRRARVSLFLGRERRFTDEEADAVLASPQGRQIAEMMR
                     YSAVGEPATVRDGLEEFRAHADADELIVVHASPTLEARLRSVTLLAGAWDLEPATVGS
                     SD"
     gene            23704..25791
                     /gene="pta"
                     /locus_tag="VLR27_06685"
     CDS             23704..25791
                     /gene="pta"
                     /locus_tag="VLR27_06685"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HSP03169.1"
                     /translation="MSEARSVYIASPEGHTGKSVVALGVLDQLTRRVRRVGVFRPITR
                     STTASDDQLLTMLCEHDGVEIPAEDSVGVTYDQVHHDPDAALATIVDRYHDVAERCEV
                     VVIVGSDYTDVAGPTELAYNARIATNLGAPVLLVVSAHERTPSDVTHLADLALAELGQ
                     AHAITVGVVANRCDPTQRDAVDAALGELGIPAWALPEVPLLSAPLLVDLLAALDGEHL
                     FGDAGMLQREAEDMLVCGMNAEHVLERLVDGQLCIVAGDRPDVLVALAAANAADGFPG
                     LAGIVLNGGYRPADHVLALVRSLDEHLPIISTELSSYEAARTVASTRGLLGGSSQRKI
                     DTALGVFEEHIDADALVAALDVPRSTVVTPLMFESLLLERARRHRQHIVMPEGDDERV
                     LRAASTLLSRHIVDLTLLGNETEVRARAAAAGVDVDGARIIDPRSSELVEGFAEEYAR
                     LRAHKGITAEVARDIVRDVSYFGTMMVHADLADGMVSGAAHSTAHTITPSFEVIKTVP
                     GTKVVSSVFFMLLADRVLVYGDCAIIPEPTVEQLADIAVSSAATAAQFGVDPRIAMLS
                     YSTGASGAGAEVDKVRAATELVRERRPDLLVDGPLQYDAAVDASVAEAKLPGSSVAGR
                     ATVFIFPDLNTGNNTYKAVQRSAGAVAIGPVLQGLNKPVNDLSRGATVRDIVNTVAIT
                     AVQAHAMKAERSS"
     gene            25788..26975
                     /locus_tag="VLR27_06690"
     CDS             25788..26975
                     /locus_tag="VLR27_06690"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HSP03170.1"
                     /translation="MTGTVLVLNSGSSSLKYQLIDPGDGTRLAVGLVDRIGEASSAIE
                     HEADGTATERHARVGSHQEALRTVIGLFDEVGPRLADAHVVAVGHRVVMGGREFAGPA
                     LIDDDVLATIERLSPLAPLHNPANLAGIEVARELLPDIPHVAVFDTAFFHDLPAVAAT
                     YALDREVAERHGIRRYGFHGTSHQYVSRTAAAFLGRDPADLRQIVLHLGNGASASAIA
                     GGRPVDTSMGLTPLEGLVMGTRTGDIDPAVVVHLTRAAGMAIDEIDALLNRRSGMRGL
                     TGHNDMREVHRLAAEGDEAARLGLDVYVHRLKKYVGAYTAVLGGLDVLAFTAGVGEND
                     AEVRARVASGLDVLGIRIDAERNAARSDEARRISPDGTPVDVLVVPTDEEIAIALEAV
                     SLL"
     gene            27019..28122
                     /locus_tag="VLR27_06695"
     CDS             27019..28122
                     /locus_tag="VLR27_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HSP03171.1"
                     /translation="MTLLTIVALVVGLVCLIAGAELLVRGAASIATRFGIAPVVIGLT
                     VVAFGTSAPELAVSVSAGFAGDGEVAFGNVAGSNIANILLILGASAVVGGLVVSQRII
                     RLDIPLLIGASVVTLLLSLDNQIGRVDGAILFAGIIAYTTWLLRVARNERADVLEEYD
                     DAVEELEVDVIDKPLPIQVALLVVGLGVLVLGSQLLVGSATDIAEELGVSDLVIGLTV
                     VAIGTSLPELATSLMAAIRGQRDIAVGNVVGSNLFNLLSVLGATALVSPDPIPVADSS
                     LQVDFPVVLAATVVLVPIIWKGFRIERWEGFVLLAFYALYVGFVVLDANDSGAVDVVG
                     PAALIVAPLVFLGFSVAGVQGWRQHQRQRQQAG"
     gene            complement(28126..>28546)
                     /locus_tag="VLR27_06700"
     CDS             complement(28126..>28546)
                     /locus_tag="VLR27_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HSP03172.1"
                     /translation="SRVQLALNVSDIDEAVAFYAKLFATEPLKRKPGYANFAIADPPL
                     KLVLFEVADGALGGTINHLGVETDSADEVEAAEARLADDGLETTGIDDTICCYASKVE
                     TWVTDPDGARWEWYVKTGDTDQMANEVVTAGGPACCG"
BASE COUNT         3941 a        10349 c        10361 g         3895 t
ORIGIN      
        1 gcctacctgc gccggtgggc ctgggaggtc ggcgtcttct tcgacggcgt ggacgccgac
       61 tcgagcgacg ccgagctggc ggcgatcgcc gaccggcacc cggtgttcgc cgtcgaggtc
      121 cacggcgcgg aggtctgagg cctccacgac ggtcgggcag actggttccg accgcgacga
      181 cgaggagccc cagtggccga ggtgaccgag acgcagctgc ccggtgtggg cgtgcggtac
      241 gagttcagga cgggcgaccg ggaccacgtc ggcgtggtgc accaccactc cggtcgccgg
      301 gagatcgtgg tctacgaccg caaggacccc gacgtcgccc gctcggtgct cgagctgagc
      361 gccgacgaca gccggaccct gagcgacctg ctcggcgcca gccaggtcac cgaggcactg
      421 gctgcggtgc agcagcgcat cgagggcctg acgctcgagt ggatcgaggt caccgaggac
      481 tcctcgatgg cgcaccgcag catcggcgac ggccagctcc gcacccgcac cggcgcctcg
      541 gtggtggccg tcatccgcgg cgacaccagc gagccggcac cgagcccgga ctacgtcctc
      601 gaacccgagg acgtcgtggt cgcggtcggg accgcagacg gactggagct cctccggcag
      661 gcgctgcgcc cgtgacgccg gtgctggccg cggtcagcgg ggagacggcc gaggcgttca
      721 tcgagatcgg cggcgtggtg gtcgtgctcg ccgtgctggc gcgcatcgcc ggccgcctcg
      781 gcatcacggc gatccccctc tacctcctgg ccgggctcgc cgtcggcgag ggcgggttca
      841 cgccgctcga ggccggtcag gacttcctgg ccatcagcgg ccagatcggc gtgctgctgc
      901 tgctgttcgc cctggggctc gagtacacgg cagacgagct gcgccacggg ctccgcaccg
      961 gcaccgggac cggggcggtg gatgcggtcg ccaacttcct ccccggcttc ctcgccggcc
     1021 ttgccctcgg gtgggagacc acctcggcag tgctgctcgg cggcgtcacg tgggtgagct
     1081 cgtccggcgt cgtcgcgaag gtcctcaccg atctgcaccg cctcggcaac cgcgagaccc
     1141 cgtcgatcct caacgtgctc gtcatcgagg acctggcgat ggccgtctac ctgcccatcg
     1201 ccgccgccct cgtcgctggc cggaccgcac tcgacacggc cgtcaccgtc ggcatcgcac
     1261 tggcggccgt gggcgtcatc ctcgtcctcg ccctcggcag cggggcccgc ctcagcgcca
     1321 tgctcgcacc cggcggtgac gagtccctgc tgctctccgt cttcggcctg accctgctgg
     1381 tgggcggcct cgccgagcag ctccaggtgt cgagcgccat cggcgccttc ctggtcggcc
     1441 tcgccctctc cggtgcggtc caacaacgag cgacgatcct ggtcgagccg ctgcgcaacc
     1501 tcttcgcgcc gatcttcttc ctctacttct ccttcggcat cgcacccgac tccctcgtcg
     1561 aggtgctcct ccccgccctg gtcctcgccg tcgtgagcgg tgccaccaag atgctctcgg
     1621 ggtggctggc cgccgcccgc gccggcgtgg gcgtccgggg tcggatgcgc gccggcaccg
     1681 cgctcatcgc gcgcggcgag ttctccatcg tgatcgcatc cctcggggtc ggcgcggtcg
     1741 acggtgccga cctcggcgcg ttcgccgcgg cctacgtgct catcaccgcg gtcctggggc
     1801 cgttggcggc caagtacgcc gaccagatcc ccatcccggc gcggctgatc cggccggctc
     1861 ccgcctgagc gccctcagct cccgctggag acgatccgcc ggccggtgat ctcctcggcc
     1921 cggatctgga cccagtcctc ccgctccacc gagtcggccc acgggtgcag ctccagcttc
     1981 tcgagctcct cggggtcctc ggcgaggtcg gcgaccccac gtacgagcac gctccagccg
     2041 gtccgctcga tcacgtcgaa gccgtcgacc tggaaggcca ccggctcggc catgagggcc
     2101 tcgtggagga gcgacccgcg cagggtccgg aacacgacgc ggtggccgac cacggcgtgg
     2161 ttgacgggga gcaccatcgg ctcgcccgcc tcgttgaacg cgacccgccc gacgggtgag
     2221 tcggcgagga gctgccagca ctcctccggg gcgatgacct ccaggccctg gtggtcgaga
     2281 ctggttcctc gggtgctgtc catgtcctga tcgtgcccgg tgcgccacac cccgcgacag
     2341 ggtcctcggt cccgacccgg ctactccgcg gccacccgca ggatgtcgtc cacgacggcg
     2401 tccaccggcg cctcgccgtc cacgaccgcg tggtgctcgc ccgaagggtg ctccaggtcg
     2461 gccagctggc tgtcgagcag gtcggccggc atgaagtgct cggtgcggcc ggccaggcgc
     2521 cgagccagct cctcgggtcc gacgtcgagg tgcaccagcg cgacgtcggg ggccgcgtcg
     2581 aggaggcgcg cccggtggtg ccgccgcagc gccgagcacg cgagcacgac ccggtcgccg
     2641 tcccgggacc ggcgctccag ctcggcggcc agcgtgtcga tccacgggtc ccggtcggcg
     2701 tcggtgaggg ccacgccggc tgccatcttc tggcggttga tctccgggtg gaagtcgtcc
     2761 gcgtcgagca ggaccgcgcc gatccgatcg gccagcgcac gcccgaccgt ggacttgccg
     2821 gcacccgaga cgccggtgag caccagcgtc tggggcgcag ccgtcatgga cccgaggcta
     2881 cggggcggac gacctcacga cgtcgggacg atgaccgccc gaccctcgat ctcgccggcc
     2941 gcgaggcgct cgtagacgga gggggcctcg tcgaggtcga agcgggtgac gtggggggtg
     3001 atgatccccg accgggccat ggcgagcacc tcgtgcagct cgggcaggct gccccagtac
     3061 gtcgtggcga ccgacacctc gtaggggatg ctgaagaagc tcatcggcag cgtcccgccc
     3121 gcgatgccca cgatggtgag ctggccgagc acccggctgg cggcggcccc gagggcgagg
     3181 gtggcgtcgg cgcccacgca gtccaggacc acgtcggctc cctggccgcc ggagaggtcg
     3241 cggatggtgg cggcggcctc gtcgtcggag acgatcccgt ggtgcgcacc gacggactcg
     3301 gcgaggcgga gcgcgtcgtc ccgttggtcg acggcgatga ccgtggcggg ggtgagggcc
     3361 cgcagcatct gcacggccat gtggccgaga ccgccgacgc cgatgaccac ggcggtcgtc
     3421 ccgggcgtca ggagcgggag ggcgcgcttg acggcgtggt acggggtgag cccggcgtcg
     3481 gtgagcggcg cggcctcggc cgggtcgagg tccccgatgg gcaccacgtg gcggacgctc
     3541 ggcacgacca tgtagggcgc catgccgccg tcggcaccga ggccgccgac ggccccaccg
     3601 gtgaggtcgg ggcgctcgca gtagttctcc atgccgagcg cgcagcgacg gcagagcccg
     3661 cagccccacg ggccgtagac cgcgacggct tcgccgacct cgagtccgga gacccccgcg
     3721 ccgagcgcct ccacccagcc ggcgttctcg tggccgaggg tgaagggcgg gttccacggc
     3781 atcatgccgg gctcgaagtc gtgcatcagg tgcaggtcgg agtggcaggc accggcgccg
     3841 ccgatgcgga gcagcacctc ccccggcccc ggctccggct ggtcgacctc gaccagctcg
     3901 ccgggctgct tgaacgcggt cagctgcagg gccttcatca gacgcctcct cgatcggtga
     3961 tcccgccacc ttcgcgcacg ggtccggcgc tgcccagggt ctcgggtccc gggcgccgct
     4021 caggggtcgt cggcgctcga gtcgagggcc cgggccgcgt cggggtcctc gtcgtcgccc
     4081 tcctggaggg cccgcacggc agcagcgccc cgcagggcca cggccacgag cggcacggcg
     4141 atgaaggccc cggcgatgcc ggcgatcgcc gcgcccgtcg tcagggccag gatgacgacc
     4201 agcgggtgga gctggagcgc cttgccgtag atgaccggcg ccaggaggtc gttgtcgagc
     4261 tgctgcacca ggatcgcgac gatcgccacg acgccggccg ccgcgctccc ggccgtgacg
     4321 agggtgacgg acacggcgat gatcccggcg gcgatcgctc cgacgaaggg gaagaaggcc
     4381 gagacgaacg tcagggccgc caccgggagc gccaggttcg cgccgacgat gatcatcgtg
     4441 atgccgatga cgatgccctc gatgacaccg agcgcggcag ccccgcggag gtagccgccc
     4501 agcgcccgcc aggccccgtt gcccgccgcc cggagctgga cctggcgtga gtcggagaac
     4561 cgggacagga gggctcgctg catcatcggg ccgtccttga ccacgaagaa ggtgacgacc
     4621 agggccaaca gcagcccggc caggacctcg ccggcgagcg tggccccgtc gacgacggcc
     4681 ccctcggaga cgctggcgct gtcggcggcc tcctcgatgc gctcgcgggc gtcgaggacg
     4741 aagtcctcgc tcagcccgag cggcccgtcg atgaaccact cctggacgtc gtcggcggcg
     4801 gtctcgagcg tggcgtcgag cccggagaac tcgtcgatga gcggcgggac gatgacccag
     4861 cccacgaaga acacggtccc gaagaacacg gcgacggtgg cgagcatggc cagcggcacc
     4921 gggacgcccc ggtcggtgaa gagcttccgc accgggatga ggacggtgga gaggagcagc
     4981 gcgatgccga ccgggagcag gacgatgcgg agctgcgagg cgacgatgcc tgtcgcgtac
     5041 acggcgagcc cgacgatcag gagcctccag ctccacgcgg tgagcacgtc gagccagcgg
     5101 ggcacgcggg gcggtccggc gggctgcacc tgatcaggac cggggtcggg gcgcgccatg
     5161 gacgtcaccc tgccacgccg gtcactcgtc gaggtcgacg tccccggtcc ggtcgatgcc
     5221 ggtggcggcc gtggtggcca cgctggtcag gttggcgcag atccgcttga ggtagcggaa
     5281 gagcatggcg cggggcacgg cgtaccgggc gggctcatcg gcgtggatca gcgcgttgac
     5341 cagcccgtcg aaggtgccca tcagctcctc ggcgcgccgg cccagcccct cgacgtcggc
     5401 ggtgtcgggt tcggccaggg cgtcggcggt gtcggtgtag agctgggaga cccggctgcg
     5461 gaagtcgagg aaccgctcgt agtcgtccgc ctcgctgaag cggaccccct ccgcagccag
     5521 gtcgaagatg ttcttggcct ggtccccgac ccgctcgagc ttcttcacca tgagcagcga
     5581 ggcgagcacg gcgccgacgt cggcgccgcc gtggacggcc gagtggacga ccagctcacg
     5641 ccgcacgctc tcctcgatgc cgttgatggc ccagtcggtg gcgcgcacct cctcgccgat
     5701 gggctcgatg tccccgtcgg tgacgaggca gctcatcgcc aggtcgaagc tgtgctggca
     5761 gcgcccgacc atcgacagga tctcgtgctg gacgtgctcg aggccttcgt caccgccgcg
     5821 gaagaacgag aacaccatgg atcaacacct catcgcagga ggatcacccc gatggccggg
     5881 atgaccagga acgtgacgat gacatacccg atgaccatcg agcgccggcg ggccgccatc
     5941 tcggcgagcg cctcggcgat ccggatcggg agccggcgca gcgccggcac cgggtagaag
     6001 agcaggatcc cggccacgtt gaacagcgtg tggaccaggg ccaccgtgag cgcctcgggc
     6061 cggtcggtgg ccagcgacgc cagcagcgcg ctgatggtgg tgccgacgtt ggcgccgagg
     6121 gtcaccgggt aggcgttgac cagcgacacg acgccggctg ccgacagcgg gatcatgatc
     6181 gaggtggtga tgctcgacga ctgcaccgcc accgtgatga tgacgccgag caggatggcg
     6241 gggacgccgc caccccggcc gagcatggcg ttgatcgagc gctccacccg ggcggcgatc
     6301 agctggcgca tgttcctcgt cacgaacgtg agggccagga agatcgcggc caggccggtg
     6361 acgaccagca ccacgccgag cagggacccc tgcaggccga cgacgtcgcc ccacagctcc
     6421 tccacccagc cgacgggcac cttgacggcg gccttgaacg ggctgtcgaa ctcggccccg
     6481 ctccccccga ggagcagctc ggtcaggaac tcggcggcgc tggacagcac gccggtgagc
     6541 agctcgatcg gcagcaggac gctgacggcc aggaggttga acgcgtcgtg caccgtcgcc
     6601 gccgcgaacg ccaggcggaa ctccgccgga cgccgcaggt ggccgagcga ggcgagcgtg
     6661 ttcgtgacgg tggtgccgat gttggcgccc atgatcatgg gcacggcgtc gtcgatgccg
     6721 aggaggccgg ccccgaccag gccgacgatg gtggaggtgg tcaccgacga cgactgggcc
     6781 agcacggtgg cgaggatgcc cacgaacagc ccggccatcg ggttcgagac gctggagaac
     6841 agctcttcct ggacgtcgct gccgagggcg ttgatgcccg actccagcag cccgaccccg
     6901 accaggaaca ggaacaggag ggcgatgacg acgccggcgc ggatgggcgc ggggaccggg
     6961 cgttcggtcg ctgcgtcggt ggtggagggg cgggagctga ccacgggcgg cctcgacgtc
     7021 ggggagcggc ggcgtgaccc ttgcacaccg ccgttcaccc gtacgacatc gcccgtccac
     7081 ctcccgtcca cctggcggga cgggtccgcc ggtgggcgcc gcggccgctc agcgcttgct
     7141 ggtccgctgg taccgggaga ccgagagcgg ggcgaacacg gccacggtga tcacgaccca
     7201 gatcagggtg tagagcaccg ggttctgcag cgaccacgcc gtcggctcgg cggtgccggc
     7261 cgggatgttg ccgaacagct cgcgtgccgc ctgcgtgacc gcggagaccg ggttccactc
     7321 ggcgaacgtc tgcagcgggc cgggcaggtc ctcggccggc acgaacgtgt tggccacgaa
     7381 cgtgagcggg aagatcacca tgaacgaggc gttgttgatg acctccacgc tcggcacgag
     7441 gagcgcgacc caggccatga cccaggagat ggcgtaggcg aacagcagca gcagggcgaa
     7501 cccggcgacc gcctccagga tcgagccccg gatgcgccag cccaccgcca ggccggtgag
     7561 ggccatgatc gccaccgaca gcacgttgta gatcacgtcg ctggccgtgc ggccggcgag
     7621 caccgccgag cgggacatgg gcagcgactt gaaccggtcg atgatgccct tctgcatgtc
     7681 ctcggcgatg ccggcgccgg tgaaggtggc tccgaagagg acggtctggg cgaagatgcc
     7741 ggcgatcagg aactcgcggt agctggtgcc ctcgatgtcg atcgagttgc cgaacacgta
     7801 ggcgaacagc agcacgaaca tgatcggcga cagcagcacg aacacgagga cctcggggac
     7861 ccgcgtgatc ttgatgacgt tgcgatgggc gatgacgccg atgtcgctga gcgtgtcccg
     7921 ccacccgatg cggctgtcgg tggtcgggtc ttcggtgatg gtggtctggc tcatgccgtc
     7981 accccctctc gctcggtgcg gtccgaggcg tcgtcggcct cctcggcgac gtgcccggtc
     8041 agcgtcagga acacgtcgtc gagcgtggga cgtcggatgc ccacgtcgct gatctcgatc
     8101 tggcggtgcc gcagctcctg caggacgtcg gtcagcacgt cgacgccgcc gtcgatgggt
     8161 gcctgcagcg accgggccgg cacctgcacc tcgatgtcgc cggcagcgtg ggcggcgacg
     8221 acctggtggg cctcgtcgag gcgagccggg tcggcgatca cgagctcgat ccgctcgcct
     8281 ccgacctggg ccttgagctg gtcggcggtg cccgacgcga tggcacggcc gtggtcgatg
     8341 acgacgatgt cgtcggcgag ctggtcggcc tcctcgaggt actgggtcgt cagcaggagc
     8401 gtggcgccgc gcgagaccag ctcgcggatg accccccaca gctcgatgcg gctgcgcgga
     8461 tcgaggccgg tggtcggctc gtcgaggaac agcaccggtg gctcggcgac cagcgcgccg
     8521 gccaggtcga ggcggcgccg catgccgccc gagtacgtct tcaccgggcg gtcgccggca
     8581 tcggtgaggt cgaaccgctc gagcagctcg gcggcccgct gcttcgagcg ggtccggccg
     8641 agtcggtaga gccggccgat catgaacagg ttctcccggc cggtgagcac ctcgtccacc
     8701 gcggcgaact gcccggagag cccgatgcgt cgccgcacct cgcccggctg ggtgcgcagg
     8761 tcggcgccgg cgatggtggc gctgccctcg tcgggctcga gcagcgtggt caggatcgac
     8821 accgccgtgg tcttgccggc gccgttgggg ccgagcaggc cgaggacgga cccttcgggg
     8881 acggtgaggt cgaggccgtc gagggccacg acgtcgccga agcgcttgcg caggccggtg
     8941 gcgacgatgg cagcgctgtg gctggtggac tggtgcatcg cgggtgctcc tggagcgggg
     9001 gtcagggcga ggagacggtc gtctgggcgt ggagacggct ctcgacggta ggactcatcg
     9061 gtcccgatcg cgtccccggt ttcccgggta ctcccctgcc gtgaccggag cggttcaggc
     9121 gaagccgacc accgggtgcg gcgtgtacgg ggcctcgagg gattcgacct cctcggcagt
     9181 gagcgtcacg tccacggcgg cgacggcatc gtcgaggtgg tggggcttgg tcgcaccgac
     9241 gatgggggcg gtgaccgccg gctggtgcag gacccacgcc agcgcgacct gggcgcggga
     9301 gatgccccgg gcctcggcga cagcggccac ctgctcgacg atctcgcggt cgctggccga
     9361 ggtgtcgtag agcgtcttgc cgaactcgtc ggtgcgggag cggtccgtct cggtgtccca
     9421 gtcccgggtg aggcgacccc gggcgagggg gctccacggg atcacgccga tgccctggtc
     9481 ctcgcagagc ggcagcatct cgcgctcctc ctcgcggttg aggaggttgt agtggtcctg
     9541 catcgacacg aacctcgtcc agccccgcag ctcggcggtg tacagcgcct tggagaactg
     9601 ccaggcccac atcgacgagg cgccgatgta gcggaccttg cccgaccgga ccaggtcgtg
     9661 cagcgcctcg agggtctcct cgatcggcgt gcgggggtcc caccggtgga tctggtacag
     9721 gtcgatgtag tcggtgccga ggcgacggag gctggcctcg cactcggcga tgatcgcctt
     9781 gcgggacagc ccggcgctgt tgggcccctt gcgcatcggc ccgaacacct tggaggcgag
     9841 cacgacgtca tcccggttcg cgtactcctg cagcgcccgc ccgacgatct cctcgctggt
     9901 cccggccgag tacacgttgg cggtgtcgaa gaagttgatg ccgtgctcga ccgcctggcg
     9961 gatgaagggg cggctggcct cctcgtcgag ggaccactcg tggttgccgg caccgggcgc
    10021 gccgaagctc atgcacccga ggcagatcct ggacacgtcg aggccggtgc ggccgagctt
    10081 cacgtactgc atgggcccac tctcgcggac cgacctccgc cggcgtcggc cggcccctcc
    10141 gtcaggggtc ggggtcgccg tggaagcggc gttggggtcg ggcgcggttg tcggggttgc
    10201 aggcgacgcg tccgttgtcg gggcgggtgc ccccaccctg ggtgacgggg tcgatgtggt
    10261 cgatgtcgca gtactcggcg ggccggtcgc agccgcgggc ggtgcagtgg cggtcgcgga
    10321 cctggatggc ccggcggagc ttgccggtga agaaccggtg ccgttcgttg taggcgatcg
    10381 ggatctcgcc gggtccgtac acgtagcgct ccagatccgg atcgtcctgc aggatccggg
    10441 cgagctggcg ggccgacagg ggggtctcgt tccacagctc ggccagacca ccagcgaggg
    10501 catcgaggcc ggccacgacg accacaagtg ggcggagccg ggctgccgcg gaggggttgg
    10561 cggtgtgggc gcgctgggcc atgaccacga gcgcatcagc acgtcgctgg gcgggggtgc
    10621 gggccagctc gctcgacaag ggttcccggc cgagcgtggt cttggcctcg tgccagtcgg
    10681 cgtggaacag ctcttcgacg atgccgtcga acaccgtctt gaacgccgcg ccaccgatgg
    10741 catccaacac cgcatcgatc accacggcgc cttggaaggt ggccgacacg tgcgcgtcgc
    10801 ggtctcgttc gatccgctcc ggctcacccg agtcgggatc gacctgcaga cgccacttcg
    10861 ccaggacctt gcgcaggccg cgcatgtcca gatccacggc ttgctgggcg agccagccct
    10921 cccactcccc gaacgcctcc cgggtctcct cggtccgcac cgcagcgatc gccgtgacgt
    10981 gctcggcgcc caggtccccg gccgcccacc gggctgacac cccgggcatg tcgatgagca
    11041 cccggcccag cttcaacgac gtcgccgccg ccgcatcacc catccgcaac cgatcccggc
    11101 accactgctt cgccgtccga taccccagcg gcacatgatc accacgggcc tcgaacgagc
    11161 gcagcaaccg gtggagctcg aagtccaacc ggcgcaagca ccgggtcagc tcctccacca
    11221 gctcccccaa cccctccgcc gtgggcacct cggacacgtc gatgccggcc acgagatcga
    11281 tcgcctcgtg caaccggacc gtcgcatccc ccactgccat accgcccatt gtacgcaaaa
    11341 cgaacacgcg ttcgtcaaca gaaatagaac atttcgggcg acgaaacccg aaaccggcac
    11401 catggggttc gggtcggcga gactcccttc gatgggcacc ctgggttccc tcgggccggg
    11461 gttccgggcg ctgtaccggg gggcgctggt ctccaacgtg ggagacggca tccggctcgc
    11521 cgcgctgccg ctgctcgcga cctccctgac gacgtccccg ttcctggtcg ccgcagtgac
    11581 ggcggcccag tacctcgcgt ggctggcgtt cggccccttc ggcggcgccc tcgtcgaccg
    11641 ggccgatcgg cggcgcaccg tcctcaccac ccagctgtgg cgggggctgc tcatggccgg
    11701 cctcgggctg ctggtgtggt cgggccacgc cgcgatctgg cacgtgtgcg tcgtcgcctt
    11761 cgccatcacg atcggcgaga tcctggtcga tccctcgacg gtcgcgctcg tccccacgct
    11821 cgtcgacgac gtcgacctcg agcgagccaa cgggcgcatc gccggcgtcg agatcgtcac
    11881 caacgacttc gcaggcgcac ccgtcggggc cgccctgtat gcgttggcgc cctggctgcc
    11941 gttcgtcgtc gacgcgtgca gctacctcgg atcgctcctg ccgttccgcc gactcccccg
    12001 acggacacgc acggcaccca gctcgaaccc ggcgccgcga ctccgcaccg atgcggcgga
    12061 cggcttccgc tggctcaggc gccaccgtgt gctcgcaccg ttcaccgtgt cgcaggtcat
    12121 ctactacttc gggttctcgg cagggttcag cctgctcgtg gtgctcgtca ccgaggagct
    12181 cgacgggtca gccgtcgcgt tcggggtggt cctcgccatc ggtgccggcg gggccttcct
    12241 cggatcgctc gccggcgccc gcgtctcgtc ggcgatcgga acgcgcgcga cgctctcggg
    12301 gtgcgtcgca ctgcaaggcg ccacccttgc tgccgcgtcg atcgcgccct ctctgccggc
    12361 cgtcgccgcg ctgtggctgg cgaacgggat cccggcgggg ttgcagcgtc ccgtcgctcg
    12421 gagcatgcag cagcggctca caccgaacca cctgctcggc cgggtgaacg tcaccacccg
    12481 gatcttcacc cgcgggatca tcgtcttcgg cgccctcgcc gccggtgcgc tggcgtccac
    12541 gaccgacatc cggtggtcct tcgcggtcgg aggtgcgatc caggtcgtgg ccgcagtggc
    12601 gatgtggagg gtgctcgctc gcctccgagc gtgagcccag ccctcagagc acgtcccgac
    12661 gctggaccac gagcagcgcc acggccgccg gaacggcagc ccagaggcac atggcgacgg
    12721 cagcggccag ggcgccgacc tcggcagacg cgcccgacag gaccgtggcg agggtggcgg
    12781 gcatccacga tgcggtcccg ctggcgagct gcaccacgag cggcggggcc accagcagga
    12841 cgaggaccgc gccggtgacc gcgccgccgg tgttgcggac cagggcgccg atgccggctc
    12901 cgagcacggc accggccgcg ccgcacaggg tcgaggcggc gagcacccgg gcgacaccgc
    12961 cgatcgacag caggaacccg aactcggtgg tgggcagggc gagcgccacg gcgaggatcg
    13021 tggtcccggc gccgaggagc ccgaggagcg caccgccgag gccgacgcct gcgaactggg
    13081 ccagcaccgc ccggtggcgc cgtggcgcgg cgaggaagga gtggatgatg gtgccgtggc
    13141 cgtgctcccg tgccgcggcg atggcgccga agaggccggc gagcagggtg gcgttggccg
    13201 cgaaggccac gagagagcgc tgatcgtcgg cggtggagat gaaggtcttg gatccgtcgg
    13261 cgtcggtgcc gagcgcgacc gcgacggcgt tgatcccggc gaggacgatc agcacggcga
    13321 cgaacgcgag cggcatggac gtggtcgtca gctttcgcag ctcggatcgg atcaggttgc
    13381 cggccttcat gccgcttcgc tcccgccatc ggtggcgggc gtgctggtcc aggcgaggaa
    13441 gcgctcctcg agggagccgg cgtgggtcga cagctcgtgg agcacgacgc ccgcctcgaa
    13501 cgcgcaccca cccacggcgt cgatcgacag gccgcgcacc agcaaccggt cgggatcccc
    13561 gggcggctgg gtcacctcga ccgacgggcc gcaccgccgg agggcggcgg ccaggacgtc
    13621 ggcggacggc gtccgcacca cgatctcggt ctgctgcagg tcggcgaggg aggagtgggc
    13681 cacgagccgg ccggcggcga tgacgaccag gtcgtcggcg aggagctcca tctcggccag
    13741 caggtggctg gacaccagca ccgtgctgcc ccgctcggcg cacccgcgca gcaggtcgcg
    13801 catggtgtgg atcccctgcg ggtcgaggcc gttggccggt tcgtccagta ccaggaccgg
    13861 cgggtcaccg agcagcgcac cagccacgcc gaggcgctgg cgcatgccga gcgagtacgc
    13921 cccgacccgc ttgtcggcgg cggcggtgag ctcgacggtc tcgaggacct cgtccacccg
    13981 ggtgccgggg atgccggcgg cgtcgctgag cacccgcagg tggttgcggc cggtgcggcc
    14041 ggggtggaac gcgtcggcct ccaggagcgc gcccacggtc cgcaccgggt cgtcgaggtc
    14101 ggcgtagcgg cgaccgccga tcgtcgcccg cccgtcggtc ggcgcggcga gccccaggac
    14161 catctgcatg gtggtcgact tgccggcgcc gttggggccg aggaagccgg tgacccgacc
    14221 cggccggacg ctgaacgtca ggtcgtccac cacggtcgcg tcgccgtagc gcttggtgag
    14281 cccctccacc tcgatggcga cgccttcgtg cggttccccg gtcactgggc cacccccgtc
    14341 ctcgccagct gcggtcgcca gaagcgaccg tacggcaccg gtcgatcgct tcgcagcgga
    14401 tgtccggaac cgaccggctc gcgccgctac accagcactg ccagttcatg gagaccgggc
    14461 cgtcagcgcg tcgacgggga ctcgtcgctg cgaggtgggg gtatcggatg gcgatggcca
    14521 agaactgcgt gcgcaccgac ctgtcgcccg acgaggtgtt cgagcacctg ctggacccct
    14581 ggcactaccc ggactggctg ctcggcgcca gcacgatgcg ggacgtggac gacagctggc
    14641 ccgcagtggg ttcggccttc caccaccgcg tcggattcgg gccgatcaag atcaacgacc
    14701 gctccaaggt cctcgagatc gacgcgccga agcggctcgt cctccacgtc cgggcgaccc
    14761 cggcggtgca ggccgtcgtc accttcacgg tcgagcccac cgccgagggc tcggtcctgt
    14821 ggctggagga gcagccggcg atgaagatcg gccagctcct gcgcccgttg ctcgatcccc
    14881 ccacccacat ccggaacaag gcatcgctgg agaacctcgc cgacctcatg cagcgcgacc
    14941 gcgaggccga cgccgccgag tgacgcgcca gggctcagcc gagcaggcgg ctccggaaca
    15001 gctcgagcac gtcgtccagc gcctggcgcg tgggctcgcc gggctcgtcg acgaggtgct
    15061 ccgtgaccac cgagtgcggc gacatcttcg agtccgggtt cgctgcatcg tcgtcgagct
    15121 cgacggcccg gaacgcgtcg ccgagctcgc gctccaggaa gcggaagcgc tcggacggca
    15181 ccaggcgatc cgagcggaag cggagcccca gcacctcgag cccgtcggcg cagcgctgct
    15241 tcacgacggc caggtcctcg gggctgatgt cgatggcgcc cttccgcgac ctcgtcaggg
    15301 cgacgggcat cgacggctgg gacaggacgg gggcgagcag ccggtcgtcg gtggccatcg
    15361 ccagcgcgaa cccgccggtg aagcacatgc cgatggcgcc gacgccgggg ccgccgcagc
    15421 gctcgtgctc ggcctccgcc agcgcccgca gccagccgat gatcggcgac gtccgcccgg
    15481 tcgcccacac cgtgaactcc ttcgagacgc aggccgggac catgctcgtg gccatgtagc
    15541 gcaggtccgg cttcccgtcc ggtgccgggt cacggccggg ctcgccgaac aggtggggca
    15601 ggaccgccgt gcacccgatg ccggccaccc gtcgggcgaa gtcggcgacc ttcggcgtga
    15661 tgccgggcat ctcggcgatg acgaccaccg cgggcccggt gccgaggcgg aacacgtcgc
    15721 gggtggtccc ggcgtgctcg aaggtggtcc gttcgaagtc ggtgagggcg tcgtcggcca
    15781 tgggccggga cgctaggtcg ggcgggcgct cagcgctcgg aggtgggatc gaccgagggc
    15841 tggccggcgt cgctcacgcc catggcctcg gcgtctgggc cggccgggcg gtcggacttc
    15901 ggctgggcgg gccggccgtg ctcgtcgctg ccggcgacgc tcgagccgtg accgctcagg
    15961 tcctcctggt gcttgcgtcc gccggcccgg gcgacgagca gcatcacggc ggtcaggagc
    16021 acgacggcgg cgatggtcag ggcgatggtc atgggcctcc cttacccagg accgctgcgg
    16081 cccttgccgg tgccgctacc ccgggatctc caccggctcc cgggtcgtcg cctcctgcgg
    16141 tggtggcggc cccggtggcg cggggtccac gggtcgggtc ccggggtcgg tgccgcctcc
    16201 gtcgaagcgg aagggcgggt actcgtcccg catcagcgcg acgtaggcga ggacccggaa
    16261 gcaccagcgg ttcagcccca tcacgaagtg gaacagctcc cgcgggtagc ggccccggaa
    16321 cgcgagcacc accgccccga tcagggcgac gaccccgatg aggcccccac cgcccacgac
    16381 ccgccagtcg tcgtctgcgc cggcgctcca gccgccggag aagatcgaca cgatcacgag
    16441 gtggggcagg gcgagcagcc accacttcac caggaccagc ccccgcgaca ggcgctcggg
    16501 gtggtcgacc tcgaagtccg ccgggtagcc gggctcgacg ccgaggcgga acggggggta
    16561 ccggtcggtg ccgaaggcgg cgaaggcgta gaagcccacc cgccacgtcc agcgcatcac
    16621 gccgacgttg aagtcgaaga gcgggcgggg gtagcgaccg gtgaacagga tcgccacccc
    16681 cgccacgacc gtcagcacga acgcagccat ccacaggaac accagcgcca cgacgtgggg
    16741 gatgacgagc acgaccttca ccagccagag ccagcggctg aggggctcgt cgagcgtggc
    16801 gtccagccgg gccgggtagg cacgagccgg gtcgggtacc tcggagacgg gtgcacccgc
    16861 aaccggggct gcggcaggcg ccgccgatgc cgagggtggg gtcgcggcgc ggcggaggcc
    16921 gacgatgagc agcacagcgg cgacgccgag cgcgagcagg cccgcgatca cgagcccgat
    16981 ggcgatcggt gcgagcaggt cggtcttgac gccgacggcg acgtcggcac gcacgccggc
    17041 gctcccgtcg gggttcatga cgacgacggt ccagtccccg tcctcgacgt cccaggtcag
    17101 gtcttcaccc gtggcggtgg cggcccagaa gccctgatcc gccggcgggg cgggagtggc
    17161 gtcaccgggc atcgaccggt actcgacctc gaaggggcgt gtcgtgaggt cgtcgatccg
    17221 gtggtgggcg acgtcgtcga ggtagccgtc cacctccgca ctcgggccga tgccgacgaa
    17281 caggtcgccg ccgtcgggcg ccgcgacctc gaggcggacg gtgcccaggt cgtcgaaggg
    17341 gacccagtcc ccttcgcgca cgtcggtgcc gaggtcgatg tgcgccgacg tcacggcgtg
    17401 accggcgctc tcgatcgggt gcgagccggt cgcgtagtac ccggcgtcgt cgcgctgggt
    17461 cgcatgggcc cagccgagga cggcgccgga gacgagcagg gcgaagccga tgagggcggc
    17521 gagggagccg gcgaccacca ggacgacgcg cccggcgccc atggcccgcc cgttccccgg
    17581 cgcccgtggt ggtggcggtg gtggtgtggt cgcagccatg gcgcgatcat ccgctccgca
    17641 gcgccggacg tgaagggcat tccgtcacag gggcgggtgc cccaccaggt gacgtcaggg
    17701 gcgacgtggg ctgatggcag ggccgctgcg gcggcggtgg accgtcaggt acgtgaagcc
    17761 gagtcccccg gcccggatgc ggcggcgggt ccccttggcc acgtggaggg cgacgctggg
    17821 ggcgaggtcg tggaggcggt cgtcgacctc ggcctcgccg gtgccgtcga gtccgacgac
    17881 cagcacgtcg acggcgtcgt tcacgtgggc gccgacctcg tggccggcgt cgaggtgcac
    17941 gaggttcacg ttcaggtcgg cgtcgggctc gagggtccag tgcacgccgt cgccggcgag
    18001 cgggagcatc gtgcgcaggt cgagccgggc cgcatcgtgc tggctcatcc gggctgctcc
    18061 acacccccat cgtcggtgag caagcgcgcc acgcgccaac cggtgtcgat cgcccagagc
    18121 gcggccgccc ccacggcgac ggggcgagcg tcgaccagga gggcgacggc ggcgacgttg
    18181 gcgaggacga gcccgggcca ggacgccgtg gtggcgaagc cggcggtgag ccgctggtgg
    18241 ccgccggcgc gcagcaccgg cccgaagtac gcgagcgacg ccaccaggat ctgggcgaac
    18301 ccacccacgg ccagcacggc gagcacccgg ccttcggtcg tggcgtcggt gaggcgcacg
    18361 acggcgaggg ccaccaccat cgccagccac cacaccgcgc cggcgccgag ctggacgagc
    18421 cgggggccgg cccaccggag ctggcgacgt ccggggaccg ggagcgtggc ggcgacggcc
    18481 acgaggccga gggcgtagcc gagcaggccc accccggcga tgcccggtcg atcgagcaga
    18541 tgaccaccgg cggccacgag cgtggcgagg gtgagcacgg tggtgctggc ccgcacccgt
    18601 gccggcgacg cccgtcggga catcttcatg cgggcctgcg tggccacgaa gtacggcagc
    18661 gtgccggcca cgaccaggcc gacgaggccg agcacgttca cggccaggtg ggcggcgcgc
    18721 aaccgcaccc accactcgcc ggcgtccccg gcggcgatcg ccgcgcccat ggcgatgccg
    18781 gcgacgccga aggcggcggc gaccacgtaa gcctcgatgg cggggtggaa gcggtcgacc
    18841 gacgagcggc gacggatccc gaccaggatc acgccgagca gcaccagccc gaccccgagc
    18901 agcagcgcgc cggtgccggt gaccgcgtcg aggtccacct cccggcccga cgccacgccc
    18961 acggcaccga gcacgaccga ccagcgctgg gcggcgacga tccggtcacc gggcgccggc
    19021 gaggacgacc acgtgacggc gagcagctgg gtggcgccgg agatggccgt cagcagcgca
    19081 ccgaccagca gcaggtgcag cggcagccac gatcccgtgt cgtggggcac cacggcggcg
    19141 acgggcgcgg ccacggcgaa cgccgccgcg gccaggagcg tgggccgggc ctggcgctgc
    19201 gtgagggcga cccggtgggc cgtgcgatca gggggtattg ttctagtcgt gagcggaaaa
    19261 tatcggtggg tccgtgggcg gtcaacccgg cggggacccg gcacgagagg tgcggtgcga
    19321 tgaacacgct gcaggagcag gccaaggcgc tgggcgatcc gacccgccac gccatcttcc
    19381 gcatggtcgc cgcctccccc gagccggtcg acgtcgccgc gctcaccgag cacttcggcc
    19441 tgaaccacaa cgccatccgc cagcacctgg ccaagctcgt ggccgccgac ctggtgctgg
    19501 agtcccgcgc tgcaaccggc cgtccggggc gcccgcggct cacctacgtc gtggacgccg
    19561 ccgccgacag ccggtggggc gtcgtcgggc cctacgagcg gctgagccag ctgctcgccg
    19621 aggtcatccg gaccggggac agcccggtcg aggtcggccg ccgggccggg cggcgcgagg
    19681 ccgccgcgcg ccagctggac ggggacgccg aggcgctggt cggcgccgtg tgcgagacca
    19741 tcgcccgcca gggcttcgac cccgacctgc accgcacggg cgacgccgtc accgtggagc
    19801 tgcgggcgtg cccgttcatg accaccgcga ccacggaccc ggacacggtc tgcgccatgc
    19861 acctgggcat cgcccagggc atgaccgagg acgccgacgg cctcgtggtc gacgagctgg
    19921 tcgcccggga cccccggcgg gagacctgcg agctgcggat gcacatcgag ccgcgctgag
    19981 cggctcagtt cccgaagtcc cagcccttct cgctgccccg gtactgaccg aggatgttct
    20041 tggcgatgag gatgcggtgc acctcgtctg gcccgtccgc caggcgaagc gtgcgcgcac
    20101 ccgtgtacat cgccgacaag ggcaggtcgc cggtgacgcc ggcggcaccc cagacctgga
    20161 tggcccggtc caccacgcgg tggaaggcgg ccggcacgaa gaccttgatg gccgagatgt
    20221 cggtgcgggc gtcctggccg gcggccatct tctcggcgca gtcgatcgtc atcagccgag
    20281 cggactgcag gtccatgtag gagtcggcga tcatcccctg gaccatctgc ttggtctcca
    20341 gcagcccgcc gtgcacctcg cgctcggtgg tccgctcgac catcaggtcg aaggcccgcc
    20401 acatggcccc gatggagttc atgcagtggt acacgcgacc ggcgccgagg cggtcctgcg
    20461 cggcctggtg ccccgagccc cgctccccca gcgcgttgtc caccggcacc cgcacgtcgt
    20521 cgtagatgat ctcgcagtgg tcgctcgggc gcccccacac gttgatcccc cggacgatct
    20581 ccaccccggg cgtgtcggtg ggcacgatga tctgggtcat cgacgcgttg cggtcggcgc
    20641 cgccctcggc gtcctcggtg cggcacatca cgatgtagaa gtcggcccgc aggccgttgg
    20701 acgtgaacca cttgtggccg ttgatcaccc actcgtcacc gtcacggcgc gccgtggtct
    20761 tgatcgagcg ggggtccgag ccgggctggt ccggttcggt catcgagaag ccggactcca
    20821 tcgtcccctc gatgagcggc atcagccact tctgcttctg ctcgtcggtg ccgtacttca
    20881 gcaggatctt ctggttgccc gagttggggg cgaccacgcc gaacagcgcg gcggcgccgg
    20941 tggcgtagga cagcacctcg tacatgtagg ccagctcgag gaagtcgagg tcggccccgc
    21001 cgtactcggc cggcaggtgc ggcgcccaca ggccggcctg cttgaccacg tccttgatgt
    21061 gctcccgcgt cttcttggcg acctcgcgct cctcgtcgtt gagcgtggtc ccgcgggact
    21121 cggcccagat catgttctcg ttcgggagga tctcccggtt cacgatctgg gccgtcagtg
    21181 cccgcacctc gttgatgcga tcggaggcgc gagggatggg ttggacgttc atctggagca
    21241 cacggccacc gtcgcggccc gggcgaggag acggaagggt ctgaggtcac acgtcgcctg
    21301 acgcgtcggt acggttcgcc gccgaggagg tcaggtgcag gatcaggcaa cggtgctcga
    21361 acggctcgac gagctggtgc ccacgatgat cgaggacgtg cgcgacctcg tgcgcatccc
    21421 gagcgtgggg ggcaccgacg cggagaacga ggcgcaggcg gcgatggccc ggcgcttcga
    21481 gcggggcggc ctcgacgtcg accactggcg catcgacctc gacgcgatca cgtcgcaccc
    21541 cgacttcccc ggcgtcgagg tggaccgccg cgaggcctgg ggcctggtcg ggcggctccc
    21601 cggttcgggc gacggaccga cgctgctgtt cgacggccac gtcgacgtcg tccccaccgg
    21661 cgaccgcgac gcctgggccg aggacccctt cgccggcgcg ctgcgcgacg ggcgcatcca
    21721 cggtcggggc agcgccgaca tgaaggccgg cctcctcgcc gcccactggg ccgtccaggc
    21781 ggtgcgggcc gccggcgtgg agctccgcgg cgacgtcctc ctcgcgccgg tgcaggggga
    21841 ggaggacggt ggcctcggca ccttcgccct gctgcagcgg gggtggacgg cggatgcctg
    21901 cgtcatcccc gagcccaccg acctcgacgt gatcccggcc aacgccggcg ccctcacgtt
    21961 ccgcctccgc atccggggcc gggcgaccca cgccgctcgc cgggccgagg gcgtgagcgc
    22021 catcgacaag ctcgtgcccg tgctcggcgc cctcgccgac ctcgagcgcc ggcgccacga
    22081 gcaggtcgac ccgctcgccg cccgctggga gctcgcccac cccctgtcgc tcggcacggt
    22141 gcgggccggc gactgggcgt cgtcggtgcc ggacctgctg gtcgccgagg gtcggatcgg
    22201 cgtggccatc ggcgagcccg tcgaggacac ccgggctgcg ctcgaggccg ccatcgcctc
    22261 cgcctgcgcc ggcgacccct ggctgcgcga ccaccccgtc gaggtggagt ggtggggcgg
    22321 ccagttcgcc tccgggcgca tcccggccgg ctccgatctg gtcgagcggg tgcagcgcgc
    22381 ccaccgggcc ggcggcggcc acccggagca ggaggtctac ggcgccacct acggcagcga
    22441 cctgcgcctg ctcaccgagc tcggcggcat cccgacagtg cagtacgggc ccggcgacgt
    22501 gcagctggcc cacggcccgc acgagtcggt gcccgtcgac gaggtcgtca ccgccgcccg
    22561 ggcgttcgcc ctgctcgtgc tcgacgtctg cggcccggcg gaatagcccg gcacccgacg
    22621 ctgttcggcg cgtgcatgac ccgcgcgccc ctctccatcc tggatctcgc cctcatcggc
    22681 cgcgaccaga ccccgcacga cgcgctggcc ggcaccgtcg cgctggcgca gcgggccgag
    22741 gagcacggct accggcgggt ctggtacgcc gagcaccaca acatgtcgac gatcgcctcg
    22801 tcggccacca gcgtgctgat cgcccacgtg gctgcccaca cccgctcgat ccgcctcggc
    22861 gccggcggga tcatgctccc gaaccactcc ccgctcacca tcgccgagca gttcgggacg
    22921 ctcgagtcgc tccaccccgg ccggatcgac ctcggcctgg ggcgggcccc gggcagcgac
    22981 cagaacacca tgcgggccct gcgccgcgac ccccgctcct ccgactcgtt cccccaggac
    23041 gtgctcgagc tgcagggcta cctgcgcgac gagacccgca tcccgggggt caacgccatc
    23101 cccggccggg gcaccgacgt gccgctctac atcctcgggt cctccctctt cggcgcccag
    23161 ctcgccgcca tgctcggcct gccctacgcc ttcgcctcgc acttcgcgcc gcaggcactc
    23221 gagcaggcgg tcgcgctcta ccggaccgag ttccgcccgt cggagcagtt gcaggagccc
    23281 tacgtcatcg ccggcgtcaa cgtgatcgcc gccgactccg aggaggacgc cgccgagcag
    23341 cacctccgcg tgcgacgggc tcgggtgtcg ctgttcctcg gccgcgagcg gcgcttcacc
    23401 gacgaggagg ccgatgccgt gctcgcctcg ccgcagggcc gccagatcgc cgagatgatg
    23461 cggtactccg cggtcggcga gccggccacc gtccgggacg ggctcgagga gttccgggcc
    23521 cacgccgacg ctgacgagct catcgtcgtg cacgcctccc ccacgctcga ggcccggctc
    23581 cgctcggtga cgctgctggc cggggcctgg gacctcgaac cggccacggt cggctcctcc
    23641 gactgacggc ggggcggctc gggtagacca gggccgaccg tcgagcgaac gaggagcccg
    23701 cgagtgtccg aggcccgcag cgtctacatc gcctcacccg agggccacac cggcaagtcg
    23761 gtggtggcgc tcggcgtcct cgaccagctc accaggcggg tgcgccgggt cggtgtgttc
    23821 cggccgatca cccgctcgac cacggcctcc gacgaccagc tgctcacgat gctgtgcgag
    23881 cacgacgggg tcgagatccc cgccgaggac agcgtcggcg tcacctacga ccaggtccac
    23941 cacgaccccg acgccgcgct ggccaccatc gtcgaccgct accacgacgt cgccgagcgc
    24001 tgcgaggtcg tggtgatcgt cggctccgac tacaccgacg tggccggccc caccgagctg
    24061 gcctacaacg cccgcatcgc caccaacctc ggcgcgccgg tgctgctcgt ggtcagcgcg
    24121 cacgagcgca cgccgagcga cgtgacccac ctggccgacc tcgcgctggc cgagctgggg
    24181 caggcgcacg ccatcacggt cggcgtcgtc gccaaccggt gcgaccccac ccagcgcgac
    24241 gcggtcgacg ccgcgctcgg cgagctcggc atcccggcct gggcgctgcc cgaggtcccc
    24301 ctgctcagcg cgccgctgct cgtggatctg ctggccgccc tcgacgggga gcacctcttc
    24361 ggcgacgccg ggatgctgca gcgcgaggcc gaggacatgc tcgtgtgcgg catgaacgcg
    24421 gagcacgtgc tggagcggct ggtggacggc cagctctgca tcgtggccgg cgaccgcccc
    24481 gacgtcctcg tggcgctggc tgccgccaac gccgccgacg ggttcccggg cctggccggc
    24541 atcgtgctca acggcggcta ccggcccgcc gaccacgtgc tggcgctggt gcgcagcctc
    24601 gacgagcacc tccccatcat cagcaccgag ctgagctcct acgaggcggc ccgcacggtg
    24661 gcgtccaccc gcggcctgct cggcgggagc agccagcgca agatcgacac cgcgctcggc
    24721 gtgttcgagg agcacatcga cgccgatgcg ctggtcgccg ccctcgacgt gccccgcagc
    24781 accgtcgtca ccccgctgat gttcgagtcc ctgctgctcg agcgggcccg ccgccaccgc
    24841 cagcacatcg tgatgcccga gggcgacgac gagcgcgtgc tgcgagcggc gtcgaccctc
    24901 ctgtcgcgcc acatcgtgga cctgaccctc ctcggcaacg agaccgaggt ccgggcgcgc
    24961 gccgcagccg cgggggtcga cgtggacggg gccaggatca tcgatccccg gtcgtcggag
    25021 ctggtggagg gcttcgccga ggagtacgcc cgcctccggg cgcacaaggg catcaccgcc
    25081 gaggtcgccc gagacatcgt ccgcgacgtg tcctacttcg gcacgatgat ggtgcacgcc
    25141 gacctggccg acgggatggt ctcgggtgcc gcgcactcca ccgcacacac catcaccccg
    25201 tccttcgagg tcatcaagac cgtccccggc acgaaggtgg tgtcgtcggt gttcttcatg
    25261 ctgctcgccg accgggtgct ggtctacggc gactgcgcca tcatcccgga gcccaccgtc
    25321 gagcagctgg ccgacatcgc cgtctcctcg gccgccaccg ccgcccagtt cggggtcgac
    25381 ccccgcatcg ccatgctgtc gtactccacg ggtgcctcgg gcgccggtgc cgaggtggac
    25441 aaggtccggg ccgccaccga gctggtccgg gagcgccgcc ccgacctcct cgtcgacggg
    25501 cccctgcagt acgacgccgc cgtcgacgcc tcggtggccg aggccaagct ccccggctcc
    25561 tccgtggccg ggcgggcgac ggtgttcatc ttcccggacc tcaacaccgg caacaacacc
    25621 tacaaggcgg tccagcgctc ggccggtgcg gtcgccatcg ggccggtgct gcagggcctg
    25681 aacaagccgg tgaacgacct ctcccgcggt gcgacggtgc gcgacatcgt gaacaccgtc
    25741 gccatcaccg ccgtgcaggc ccacgccatg aaggccgagc ggagctcgtg accggcaccg
    25801 tcctcgtcct caactccggc tcgtcctcgc tcaagtacca gctcatcgat cccggggacg
    25861 gcacccgcct cgccgtcggt ctggtcgacc gcatcgggga ggcgagctcg gccatcgagc
    25921 acgaggcgga cgggactgcg acggagcgcc acgccagagt gggctcgcac caggaggcgc
    25981 tgcgcacggt catcggcctg ttcgacgagg tcggtccccg cctcgccgac gcccacgtcg
    26041 tggccgtcgg gcaccgcgtc gtgatgggcg gtcgcgagtt cgccgggccg gccctgatcg
    26101 acgacgacgt gctcgccacc atcgagcggc tctcccccct ggccccgctg cacaaccccg
    26161 ccaacctggc cggcatcgag gtcgcccgcg agctcctgcc cgacatcccc cacgtcgccg
    26221 tgttcgacac cgccttcttc cacgacctgc ccgctgtggc cgcgacctac gccctcgacc
    26281 gggaggtcgc cgagcgccac ggcatccgcc gctacggctt ccacggcaca tcgcaccagt
    26341 acgtgtcccg gaccgccgcc gccttcctcg ggcgggaccc ggcggacctg cgccagatcg
    26401 tcctgcacct gggcaacggc gcgtcggcgt cggccatcgc cggcggccgg cccgtcgaca
    26461 cctcgatggg cctcacgccg ctcgaagggc tggtcatggg cacccgcacg ggcgacatcg
    26521 acccggccgt cgtggtgcac ctcacgcgcg ctgccggcat ggcgatcgac gagatcgacg
    26581 cgctgctcaa ccgccggtcc ggcatgcgcg gcctcaccgg ccacaacgac atgcgcgagg
    26641 tccaccgcct cgccgccgag ggcgacgagg ccgcccgcct cggcctcgac gtgtacgtgc
    26701 accggctcaa gaagtacgtc ggcgcctaca cggcggtgct cggcgggctc gacgtgctgg
    26761 ccttcaccgc cggcgtcggg gagaacgacg ccgaggtccg ggctcgggtc gcgagcggcc
    26821 tcgacgtgct cgggatccgg atcgacgccg agcgcaacgc cgcgcgcagc gacgaggccc
    26881 gccgcatctc cccggacggc accccggtcg acgtgctcgt cgtgcccacc gacgaggaga
    26941 tcgccatcgc cctcgaggcc gtctcgctgc tctgagcgat gccgtcggcg gaccccgccg
    27001 gtggcagcat tcgcgcccat gacgttgctg acgatcgtgg cgctggtggt cgggctggtc
    27061 tgcctcatcg ccggcgccga gctgctcgtg cggggtgccg cctccatcgc cacccgcttc
    27121 ggcatcgccc cggtcgtcat cgggctcacc gtcgtcgcgt tcggcaccag cgcgccggag
    27181 ctggccgtca gcgtgagcgc cggcttcgcc ggggacggcg aggtcgcctt cggcaacgtg
    27241 gccggctcga acatcgccaa catcctcctg atcctcggcg cgtcggcggt ggtgggcggc
    27301 ctggtggtgt cccagcgcat catccgcctc gacatcccgc tgctcatcgg tgcgtcggtg
    27361 gtcacgctgc tgctgtcgct cgacaaccag atcggtcggg tcgacggcgc catcctcttc
    27421 gccgggatca tcgcctacac gacgtggctg ctccgggtcg cccgcaacga gcgggccgac
    27481 gtcctcgagg agtacgacga cgccgtcgag gagctcgagg tcgacgtgat cgacaagccg
    27541 ctgccgatcc aggtggcgct gctcgtggtc ggcctcggcg tgctcgtcct cggctcccag
    27601 ctgctcgtcg gctcggccac cgacatcgcc gaggagctcg gcgtcagcga cctcgtgatc
    27661 ggcctcaccg tcgtcgccat cgggacgtcg ctgcccgagc tggccacgtc gctgatggcg
    27721 gcgatacgcg ggcagcgcga catcgccgtc ggcaacgtgg tcgggtccaa cctgttcaac
    27781 ctgctgtcgg tgctcggcgc caccgccctc gtctcccccg acccgatccc ggtggcggac
    27841 agctcgctgc aggtcgactt cccggtggtg ctggcggcca ccgtcgtgct cgtgccgatc
    27901 atctggaagg gcttccgcat cgagcggtgg gagggcttcg tcctgctggc cttctacgcc
    27961 ctctacgtgg ggttcgtggt gctcgacgcc aacgacagcg gggcggtcga cgtggtcggt
    28021 cccgccgcgc tgatcgtggc gccgctggtg ttcctcgggt tcagcgtggc cggcgtgcag
    28081 ggctggcgcc agcaccagcg ccagcgccag caggccggct gaggctcagc cgcagcaggc
    28141 cgggccgccg gcggtgacga cctcgttggc catctggtcg gtgtcgccgg tcttgacgta
    28201 ccactcccac cgggcgccgt cggggtcggt gacccaggtc tcgaccttgc tggcgtagca
    28261 gcagatggtg tcgtcgatgc cggtggtctc gaggccgtcg tcggccaggc gggcctcggc
    28321 cgcctcgacc tcgtcggcgc tgtcggtctc cacgccgagg tggttgatgg tgccgccgag
    28381 ggcgccgtcg gccacctcga acaggaccag cttcaggggc gggtcggcga tggcgaagtt
    28441 ggcgtagccg ggcttgcgct tgagcggctc ggtggcgaag agcttggcgt agaacgccac
    28501 ggcctcgtcg atgtcgctga cgttgagggc gagctggact cgggac
//
LOCUS       DATISU010000162         9016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_426899, whole genome shotgun sequence.
ACCESSION   DATISU010000162 DATISU010000000
VERSION     DATISU010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9016
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426899"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="VLR27_06705"
     CDS             <1..1273
                     /locus_tag="VLR27_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03173.1"
                     /translation="LVLIGLTWAGGDDRLPLALPPAETMLAPAAASMAIAVGLGVVAF
                     EVDLRQYHFGWRQVASIVAGVALVVGAIPTMLDAGNGRWYLPAGDLQSTFGFLEGEEP
                     GFRVLWIGHPDVLPLPGHPLDLPDRDEPTSDHVVYATSQDGLPSITDGWPGSEDGPTQ
                     LIEDALVLAARGETSRLGRLLAPMAIRYVVVPTASAPRPLGGVQRPAPVQVTGTLADQ
                     LDLAAVPVNPAYDVYRNEAALPSPAMIPATAGSGAAFDVTAGDLVGAEAVLGERVGPT
                     TRTGEVPAGARIHHASASSDRWELLVEGRPAPSTKLFGWAQGFEPATSGEATLRYDTA
                     PVRYGVLAFQALFWLVAIRFARRARHRAPVVLPSSEPALTSEPVPPAEPAGEPADWLA
                     PAEEWPADPVPALLDELEPEPAPEPDEEERP"
     gene            1270..3093
                     /locus_tag="VLR27_06710"
     CDS             1270..3093
                     /locus_tag="VLR27_06710"
                     /inference="COORDINATES: protein motif:HMM:NF040160.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HSP03174.1"
                     /translation="MNRRLPVLVLLIAFVVAGVVLDRDAGDDTAVEAVPVAPSDFPMA
                     PSPTALASTWFCAGGTADEESFADHVVTMLNTTEDPLDVDVTAFPGIVAAPAPSAEAD
                     ALDPDAEPEAESEEDAEEADEEPATDPASVSTEPVVRRIEVGARSRVRLALTDLVTAP
                     VASALVESVGGGLVVEHEVSSIHGIDAKPCATGASDQWHFAWGTTAREARELLVLFNP
                     VPDDAIVEGVFSTEDTVREPGRFAGGLVVPGRSTVGIDLGDDVTRRDEVAATLTTRTG
                     RLVVDRIVRVNEEEGDRGLTVQTGVNEPQRTWVFPDGLTSEEVNERFVVYNPGDELAE
                     VEIELRLDRPEENGIPAPVELSIAPGAHATVDMNEDGRLPSGVPHAAVVRSANDVPVV
                     AERVLSSRRESGRRGLSVTTGSPVEATEWTFAAGSTQEPRAQSLTIVNLDPQVLAEVD
                     VVAVVGGRELPVAEMQGVEIEAGGRLSVDLTELIANRYDLAVVVRATEPVVVERTLAQ
                     VGEDDRGISTSVGVPSPDGARVPPDPVDVGLDADLGDELDEAPTEDDEGIPVAPDDVE
                     LPSPDETIVVDPDDESEAPAEGDDTTTTTTEATDAADEGDG"
     gene            3093..3560
                     /locus_tag="VLR27_06715"
     CDS             3093..3560
                     /locus_tag="VLR27_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSP03175.1"
                     /translation="MERLVIVVVVAIVAVAVALVVQRRQPADAPVRTGYNVPGQLHRP
                     DFTRPDADWLVVVFSSATCETCAGMWDKVQVLESDAVAVQEVEVTAHRELHDRYRIDG
                     VPTTVVADAEGVVRASFLGPATATDLWAALAELREPGTVPDSCHEHVHDEDDA"
     gene            complement(3606..5528)
                     /gene="ftsH"
                     /locus_tag="VLR27_06720"
     CDS             complement(3606..5528)
                     /gene="ftsH"
                     /locus_tag="VLR27_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HSP03176.1"
                     /translation="MQPQSPPPPPPPPGRESKGGARGASARLGGMPRWSLWVLVGVLL
                     LGFVLAGFFPGEPREDLTYSEFRTAVLDGQVQDIEIDNNNGDIDGELDDGTLFTTTGP
                     VDGGIPDEDLRILRDQGVEVSYVTPSPSFIAAILPYIIVFGLFIGFFIWMQRRAQGQM
                     SGVMNIGKSKAKPYDTERPGTTFDDVAGYEGVKQEITEVVDFLRHPEKFAEIGARIPK
                     GVLLVGPPGTGKTLIARAVAGEAGVPFLSVTGSDFMEMFVGVGASRVRDLFQTAKKMG
                     KAIIFVDEIDSIGRKRGAGLGGGHDEREQTLNQMLSEMDGFEATEGIVMMAATNRPDI
                     LDPALLRPGRFDRQVVVPLPESEERLAILKVHVKSKRLAGDVDLHTVAKGTPGMSGAD
                     LANLVNEAALYAVRAGDEAIHMRHFENARDRVLMGQRRESMALSDQEKEVIAYHEAGH
                     AVCAAVLPHADPVHKVTILPTGMALGVTQQLPEERHIYRQDYIEDSLVVRMGGRIAEE
                     VVYGVYSTGANNDLTGATDLATKMVREWGMSERVGPQAWSGGNQVFLGESMMASKEYS
                     DDTARVIDEEVERILREQQQRCRDTLHENRNGLDLVARALLERETIDGTEVSRLIELG
                     RGAVDDVPERESVTTD"
     gene            5637..5864
                     /locus_tag="VLR27_06725"
     CDS             5637..5864
                     /locus_tag="VLR27_06725"
                     /inference="COORDINATES: protein motif:HMM:NF023430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HSP03177.1"
                     /translation="MSASCSRPACQRSPAATLTYDYGSRVATVDHLAPAHPMQYDLCT
                     EHTERLSVPNGWSLVDRRIGNVVDLHGQLAS"
     gene            5873..6583
                     /locus_tag="VLR27_06730"
     CDS             5873..6583
                     /locus_tag="VLR27_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HSP03178.1"
                     /translation="MTDPAPRWGAVDLAVAFVVAQLASALGFALFAAAQGIPVGELDT
                     DALTIGEVALLQVPLWLGLLGVPLVATRLRGNGPVRDLGLRTTLRDMPIGLAIGVACQ
                     LVLVPLVTLPIFIFTDTDQEALEAPARELTDKAQGPGVLVLVLVVVVAAPLAEEVFFR
                     GMLQRTLARYLPIWPAMLITSVLFGISHFQALQLPALAAFGLVLSVLAHRTGRLGPSI
                     WAHVGFNATTVVALVAGG"
     gene            6624..>9016
                     /locus_tag="VLR27_06735"
     CDS             6624..>9016
                     /locus_tag="VLR27_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03179.1"
                     /translation="MSPVARRTIDALVVAASALFVFWQLHPDLLLAETTPAGGDMGAH
                     VWAFAYLRDHLLPDLRLAGWTPDWYAGFPVFQFYMVVPFLGMVALNAGLGGALAILPA
                     AASLLIAGRAAGRWVRDRTELAVTAGLALVALLAGLWGGFGPTFDLVLIVAGVAAAVV
                     AGRFGRHPRRVPLAVAAGLVAVLGVAVPYGPSFKLVSVAGVVAMPVAAYAFGRLARFP
                     HPTPALLSVTTLAFLFEKSFTIYGGNIASTMAGEFAFSISLSLAIVYLGVVVHGLRTG
                     THRATAAALLALVGLCHLIPAFFALGATLVLLVVHLVAEPAREGAEARRATLAGGLAA
                     TSVVLGGVVALVTGLAGTGPVVAVTVVLLVVVAIVWLTRDGADASDAGVERRPFALGR
                     LWWLLSVGVVAALLAAWWVLPFFWRRTLVNDMGWEKIAVHREGTALWAWFTDDVWPAL
                     VPGDLRLFAALALVGAVLSVAFRSRIGVALVVTGLGLALAFVFLPQGRLWNARLLPFW
                     YLVVFWMAAVGIGEIVRALAQVLAPDPRQPRRWVTLTAAPLALLLLGGFLAGQLRNAP
                     GGATNADGTYRLVDSVGVPGLFSLRVPDALGIDVGGRNFVTDWARWNYSGYERKEAYP
                     EYEGIVSTMAELGAANGCGRSLWEYSSDLNDYGTPMALMLLPHWTDGCIGSMEGLYFE
                     ASSTTPFHFLMQSELSQAPSRAQRDMPYRDLDVDSGVEHMQLMGVRYYLARSPEAKAA
                     AAGHPELTEVAADGPWLVYEVAGADLVEPLAHEPAVTTASGSQDEWLGCKEGEEPCPG
                     "
BASE COUNT         1081 a         3416 c         3188 g         1331 t
ORIGIN      
        1 cctggtgctc atcgggctga cctgggccgg gggcgacgac cggctgcccc tcgccctgcc
       61 gccggcggag acgatgctgg ccccggcggc ggcgtcgatg gccatcgcgg tcggcctcgg
      121 cgtggtcgcc ttcgaggtcg acctgcgcca gtaccacttc gggtggcgcc aggtcgcgtc
      181 catcgtggcc ggcgtggcgc tggtcgtcgg cgccatcccc accatgctcg atgccggcaa
      241 cggccgctgg tacctcccgg cgggcgacct ccagagcacc ttcgggttcc tggagggtga
      301 ggagcccggc ttccgggtgc tgtggatcgg ccaccccgac gtcctgcccc tgcccgggca
      361 ccccctcgac ctgcccgacc gggacgagcc cacctccgac cacgtcgtct acgccaccag
      421 ccaggacggt ctgcccagca tcaccgacgg ctggccgggc tcggaggacg ggccgaccca
      481 gctcatcgag gacgcgctcg tcctggcggc ccgaggcgag acgagccggc tcggccgcct
      541 cctcgccccg atggccatcc gctacgtggt cgtgcccacc gcctcggccc cgcgcccgct
      601 cggcggcgtg cagcggcccg cgccggtcca ggtcaccggc accctggccg accagctgga
      661 tctcgccgcg gtgccggtca accccgccta cgacgtgtac cgcaacgagg ccgccctgcc
      721 gtcgccggcc atgatcccgg cgaccgccgg ttcgggcgcc gcgttcgacg tcactgccgg
      781 cgacctcgtc ggtgccgagg cggtgctcgg cgagcgggtc ggcccgacca cccgcaccgg
      841 cgaggtgccc gctggcgccc gcatccacca cgcctcggcg tcctcggacc gctgggagct
      901 gctggtcgag gggcgccccg ccccgagcac gaagctgttc ggctgggcgc agggcttcga
      961 acccgccacc agcggcgagg ccaccctgcg ctacgacacc gcacccgtcc gctacggcgt
     1021 gctcgcgttc caggcgctgt tctggctcgt cgcgatccgc ttcgcccgcc gtgcccggca
     1081 tcgggccccg gtcgtgctgc cgtcgtcgga gccggcgctg acctccgagc ccgtcccgcc
     1141 cgccgaaccg gccggggagc ccgcagactg gttggcaccg gccgaggagt ggccggcaga
     1201 tcccgtgccg gcgctgctcg acgagctcga gccggaaccc gcaccagagc ccgacgagga
     1261 ggaacgaccg tgaaccgtcg cctgcccgtc ctggtgctgc tcatcgcgtt cgtcgtggcc
     1321 ggcgtcgtgc tcgaccgtga cgccggagac gacaccgcgg tggaggcggt gccggtcgct
     1381 ccctccgact tcccgatggc gccgtcaccg accgcgctcg cctccacctg gttctgcgcc
     1441 ggcggcaccg cggacgagga gtccttcgcc gaccacgtcg tcacgatgct gaacaccacc
     1501 gaggaccccc tcgacgtcga cgtcaccgcc ttccccggca tcgtggccgc ccctgccccg
     1561 agcgccgagg ccgacgccct ggaccccgac gccgaaccgg aggccgagtc cgaggaggat
     1621 gcggaggagg ccgacgagga acccgccacc gacccggcct cggtcagcac cgagccggtg
     1681 gtgcggcgca tcgaggtcgg agcccgcagc cgcgtccgcc tggccctgac cgatctggtc
     1741 accgctccgg tcgcatcggc cctcgtggag tccgtcggcg gcggcctcgt ggtcgagcac
     1801 gaggtgtcca gcatccacgg catcgacgcc aagccctgcg ccacgggtgc cagcgaccag
     1861 tggcacttcg cctgggggac cacggcccgc gaggcccgcg agctgctcgt gctgttcaac
     1921 ccggtccccg acgacgccat cgtcgagggc gtgttctcca ccgaggacac cgtgcgcgag
     1981 ccgggccgct tcgccggcgg gctcgtcgtc ccggggcgca gcaccgtggg catcgacctc
     2041 ggcgacgacg tgacgcgccg cgacgaggtc gcggcgacgc tgacgacccg caccggccgg
     2101 ctcgtggtcg accgcatcgt gcgggtcaac gaggaggagg gcgaccgggg cctcaccgtg
     2161 cagaccggcg tcaacgagcc gcagcgcacc tgggtcttcc ccgacggcct cacctccgag
     2221 gaggtcaacg agcggttcgt cgtctacaac ccgggcgacg agctggccga ggtggagatc
     2281 gagctgcggc tcgaccgtcc cgaggagaac ggcatcccgg caccggtcga gctgagcatc
     2341 gcgccgggcg cccacgccac cgtggacatg aacgaggacg gccggctgcc ctcgggcgtg
     2401 ccgcacgccg ccgtggtccg atccgccaac gacgtccccg tcgtggccga gcgggtgctg
     2461 tcgtcgcggc gggagtccgg acgccggggc ctgtcggtga ccaccgggtc gcccgtcgag
     2521 gccaccgagt ggaccttcgc ggcgggcagc acccaggaac cccgggccca gtccctcacc
     2581 atcgtgaacc tcgatcccca ggtcctcgcc gaggtcgacg tggtcgcggt cgtgggcggc
     2641 cgcgagctgc ccgtggccga gatgcagggc gtcgagatcg aagccggcgg ccggctgagc
     2701 gtggacctca ccgagctgat cgccaaccgg tacgacctcg ccgtggtcgt gcgggccacc
     2761 gagcccgtcg tggtcgagcg gacgctggcc caggtgggcg aggacgaccg cggcatctcc
     2821 acttctgtcg gcgtcccctc gccggacggg gcccgcgtcc cacccgaccc ggtcgacgtc
     2881 ggcctcgatg ccgacctcgg cgacgagctc gacgaggcac cgaccgagga cgacgagggc
     2941 atcccggtcg ctcccgacga cgtggagctc ccgtcgccgg acgagaccat cgtcgtcgat
     3001 cccgacgacg agtccgaggc gccggccgag ggcgacgaca cgaccacgac gaccaccgag
     3061 gcgaccgacg ccgccgacga gggcgacggc tgatggagcg cctcgtcatc gtggtggtcg
     3121 tcgcgatcgt cgcggtggcg gtcgcgctcg tcgtgcagcg ccgccagccg gcggacgcgc
     3181 cggtgcgcac cggctacaac gtccccggcc agctccaccg gcccgacttc acccgacccg
     3241 acgccgactg gctcgtcgtg gtcttctcgt ccgcgacgtg cgagacctgc gcgggcatgt
     3301 gggacaaggt ccaggtgctg gagtccgacg ccgtcgccgt ccaggaggtc gaggtcacgg
     3361 cccaccggga gctgcacgac cgctaccgca tcgacggcgt gccgaccacc gtggtggccg
     3421 acgccgaggg cgtcgtgcga gccagcttcc tcgggccggc gacggccacc gacctctggg
     3481 ccgccctggc cgagctgcgc gagcctggca cggtcccgga cagctgccac gagcacgtcc
     3541 acgacgagga cgacgcctga ccgcgacccg ttccgtggtc gctgccagaa tcgttccgga
     3601 ggtgctcagt cggtggtgac cgactcccgc tcgggcacgt cgtccacggc accacggccg
     3661 agctcgatga ggcggctgac ctcggtcccg tcgatggtct cgcgctcgag cagggcccgg
     3721 gccacgaggt cgaggccgtt gcggttctcg tgcagcgtgt cgcggcagcg ctgctgctgc
     3781 tcgcgcaaga tccgctcgac ctcctcgtcg atcacccggg cggtgtcgtc ggagtactcc
     3841 ttggacgcca tcatcgactc gccgaggaag acctggttgc cgccgctcca cgcctgcggg
     3901 ccgacccgct cggacatgcc ccactcccgc accatcttgg tggcgaggtc ggtggcgccg
     3961 gtgaggtcgt tgttggcgcc ggtggagtag acgccgtaga cgacctcctc ggcgatgcgg
     4021 ccgcccatgc ggaccacgag ggagtcctcg atgtagtcct ggcggtagat gtggcgctcc
     4081 tcggggagct gctgggtgac gccgagggcc atgccggtgg gcaggatcgt gaccttgtgg
     4141 accggatcgg cgtgggggag cacggcggcg cacacggcgt ggccggcctc gtggtaggcg
     4201 atgacctcct tctcctggtc ggagagggcc atggactcgc gccgctggcc catgaggacg
     4261 cggtcgcggg cgttctcgaa gtggcgcatg tggatggcct cgtcgccggc ccgcacggcg
     4321 tagagggcgg cctcgttgac caggttggcc aggtcggcac cgctcatgcc cggcgtgccc
     4381 ttggccaccg tgtggaggtc gacgtcgccg gcgaggcgct tggacttgac gtgcaccttc
     4441 aagatggcga gccgctcctc ggactcgggc agcggcacga cgacctggcg gtcgaagcgg
     4501 cccggccgga gcagggcggg gtcgaggatg tcggggcggt tggtggccgc catcatcacg
     4561 atgccctcgg tggcctcgaa gccgtccatc tccgacagca tctggttcag cgtctgctcg
     4621 cgctcgtcgt ggccgccacc gagcccggcg ccgcgcttgc ggccgatgga gtcgatctcg
     4681 tccacgaaga tgatcgcctt gcccatcttc ttggccgtct ggaacaggtc gcgcacgcgg
     4741 ctggcgccga cgcccacgaa catctccatg aagtccgagc cggtcaccga caggaacggc
     4801 acgccggcct cgccggccac cgcccgggcg atgagggtct tgccggtgcc gggagggccg
     4861 acgagcagga cgcccttggg gatgcgggcg ccgatctcgg cgaacttctc ggggtggcgc
     4921 aggaagtcga ccacctcggt gatctcctgc ttgacgccct cgtagccggc gacgtcgtcg
     4981 aaggtcgtgc cggggcgctc ggtgtcgtag ggcttggcct tcgacttgcc gatgttcatg
     5041 acgccgctca tctggccctg ggcccggcgc tgcatccaga tgaagaagcc gatgaacagg
     5101 ccgaacacga tgatgtaggg gaggatggcg gcgatgaagc tgggcgacgg ggtcacgtag
     5161 ctgacctcca cgccctggtc ccgcaggatg cgcaggtcct cgtcggggat gccgccgtcg
     5221 acggggcccg tggtggtgaa cagcgtgccg tcgtcgagct cgccgtcgat gtcgccgttg
     5281 ttgttgtcga tctcgatgtc ctgcacctgg ccgtcgagga cggcggtgcg gaactcgctg
     5341 taggtgaggt cctcccgggg ctcgcccggg aagaacccgg cgagcacgaa gccgagcagg
     5401 agcacgccga ccaggaccca gagcgaccag cggggcatcc cgcccagccg ggcgctggcg
     5461 ccccgcgcgc cacccttgga ctcgcggccc ggcggcgggg gaggcggcgg aggcgactgc
     5521 ggctgcatgg ggccaggata cgggtcaggg ccgacggtcg gacgtgcagc cccgccgatc
     5581 gcgccccgtg gcggtgtcca ccgcggcggg gatggccgtg gctaccctgc tctcccgtga
     5641 gtgccagctg ctctcgtccc gcgtgccagc ggagcccggc cgcgaccctg acctacgact
     5701 acgggtcccg ggtcgccacc gtcgaccacc tcgcgcccgc gcacccgatg cagtacgacc
     5761 tgtgcaccga gcacaccgag cggctctcgg tccccaacgg ctggtccctc gtcgaccgcc
     5821 gcatcggcaa cgtggtcgac ctccacggcc agctcgcctc ctgagtccaa acgtgaccga
     5881 tccggccccc cgctggggcg cggtcgacct cgcggtcgcc ttcgtcgtgg cccagctcgc
     5941 ctccgccctc ggcttcgccc tgttcgccgc ggcccagggg atcccggtcg gtgagctcga
     6001 caccgacgcc ctgacgatcg gcgaggtcgc gctgctccag gtcccgctgt ggctgggcct
     6061 gctgggcgtc ccgctcgtcg cgacccggct gcggggcaac gggccggtgc gcgacctcgg
     6121 gctgcgcacg acgctgcgcg acatgccgat cgggctcgcc atcggcgtgg cctgccagct
     6181 ggtgctggtc ccgctggtga cgctgcccat cttcatcttc accgacaccg accaggaggc
     6241 gctcgaggcg ccggcccgcg agctcaccga caaggcccag gggcccgggg tgctcgtgct
     6301 cgtgctggtc gtggtggtcg ccgcgccgct ggccgaggag gtcttcttcc gggggatgct
     6361 ccagcggacc ctggcccgct acctgccgat ctggccggcg atgctcatca cgtcggtgct
     6421 cttcgggatc agccacttcc aggcgctgca gctgccggcc ctcgccgcct tcggcctggt
     6481 gctgtcggtg ctggcccacc gcaccggtcg cctcggcccc agcatctggg cccacgtcgg
     6541 cttcaacgcg accacggtcg tcgcgctcgt cgccggtggg tgatcggtcc gacccgggac
     6601 cggggtggga ggatgtctcg ccgatgagcc cggtcgcccg ccgaacgatc gatgccctgg
     6661 tcgtcgcggc cagcgcactg ttcgtcttct ggcagctgca ccccgacctg ctcctggccg
     6721 agaccacccc ggccggcggt gacatggggg cccacgtctg ggccttcgcc tacctgcgcg
     6781 accacctgct gccggacctg cgcctcgccg ggtggacccc cgactggtac gccggcttcc
     6841 ccgtcttcca gttctacatg gtcgtcccgt tcctcgggat ggtggcgctg aacgccggac
     6901 tcggcggggc gctcgccatc ctgccggccg ccgcgtcgct gctgatcgcc ggccgtgctg
     6961 ccggccgctg ggtccgggac cgcacggagc tggccgtcac cgccgggctc gcgctggtgg
     7021 cgctcctcgc cgggctctgg ggcggcttcg gcccgacgtt cgacctggtg ctcatcgtcg
     7081 ccggcgtggc cgccgccgtg gtggcgggcc ggttcgggcg acacccccgg cgcgtgcccc
     7141 tcgccgtcgc cgccgggctg gtggccgtgc tcggcgtggc cgtgccctac gggccctcgt
     7201 tcaagctggt gtcggtggcc ggggtggtgg ccatgcccgt cgccgcctac gccttcggcc
     7261 ggctcgcccg gttcccgcac ccgaccccgg ccctgctgtc ggtcaccacc ctcgccttcc
     7321 tgttcgagaa gtccttcacc atctacggcg ggaacatcgc ctcgacgatg gccggcgagt
     7381 tcgcgttctc gatcagcctc tccctcgcca tcgtctacct cggcgtcgtc gtccacgggc
     7441 tccgcaccgg cacccaccgg gccacggcag cggcgctgct cgccctggtc ggcctctgcc
     7501 acctgatccc cgccttcttc gccctcgggg ccaccctggt gctgctcgtc gtccacctcg
     7561 tcgccgaacc ggcccgggag ggggccgagg cccgccgggc gacgctcgcc ggcgggctgg
     7621 ctgcgacctc cgtggtgctg ggcggcgtgg tggccctcgt caccggtctc gccggcaccg
     7681 gaccggtggt ggccgtcacc gtcgtgctgc tggtggtcgt cgcgatcgtc tggctcaccc
     7741 gggacggcgc agacgcgtcc gacgcgggcg tcgagcgcag acccttcgcc ctcgggcggc
     7801 tctggtggct cctcagcgtc ggcgtcgtcg ccgccctgct cgccgcgtgg tgggtcctgc
     7861 ccttcttctg gcgacggacc ctcgtgaacg acatggggtg ggagaagatc gccgtccacc
     7921 gcgagggcac tgccctctgg gcgtggttca ccgacgacgt gtggccggca ctcgtcccgg
     7981 gcgacctccg gctcttcgcc gccctcgccc tggtcggcgc ggtcctgtcg gtggcgttcc
     8041 gcagccgcat cggggtcgcc ctcgtcgtca ccggcctggg cctggccctc gccttcgtct
     8101 tcctgcccca gggtcggctg tggaacgccc gcctcctgcc gttctggtac ctcgtcgtgt
     8161 tctggatggc cgcggtcggc atcggcgaga tcgtccgggc cctcgcccag gtgctcgccc
     8221 ccgacccccg ccagccccgg cgctgggtca ccctcacggc ggcgccgctg gccctcctgc
     8281 tgctcggcgg cttcctggcc ggccagctcc gcaacgcccc cggcggggcc accaacgccg
     8341 acggcaccta ccggctcgtc gactcggtcg gggtcccggg cctgttctcc ctgcgcgtgc
     8401 ccgacgcgct cggcatcgac gtcggcgggc ggaacttcgt caccgactgg gcccggtgga
     8461 actacagcgg gtacgagcgc aaggaggcct acccggagta cgagggcatc gtctcgacca
     8521 tggccgagct cggcgcagcc aacggctgcg gccgctccct gtgggagtac agctcggacc
     8581 tgaacgacta cgggaccccg atggccctca tgctcctgcc gcactggacc gacggctgca
     8641 tcggctcgat ggagggcctg tacttcgagg cgtcctccac cacaccgttc cacttcctca
     8701 tgcagtccga gctgtcgcag gcccccagcc gggcccagcg cgacatgccc taccgcgacc
     8761 tcgacgtcga ctcgggcgtc gagcacatgc agctcatggg ggtgcgctac tacctggccc
     8821 gcagccccga ggcgaaggcg gctgccgccg gacacccgga gctgaccgag gtcgccgcgg
     8881 acgggccgtg gctcgtctac gaggtggccg gtgccgacct cgtggagccc ctcgcccacg
     8941 aacccgccgt caccaccgcg tcggggtccc aggacgagtg gctgggctgc aaggagggcg
     9001 aggagccgtg cccggg
//
LOCUS       DATISU010000163         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_426958, whole genome shotgun sequence.
ACCESSION   DATISU010000163 DATISU010000000
VERSION     DATISU010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426958"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VLR27_06740"
     CDS             complement(<1..492)
                     /locus_tag="VLR27_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HSP03180.1"
                     /translation="MFEALSDRFDGIFTRIKGKGKLREEDVDETLREIRLALLEADVN
                     FQVVKNLVSRIRERVVGIEVSQALNPAQQVIKIVNEELVESLGGETIKISYASKPPTV
                     VLMAGLQGSGKTTNSAKLARWFKAQGRNPLLVGADLQRPAAVQQLRTLAEQVGVPVYS
                     ADDE"
     gene            complement(505..933)
                     /locus_tag="VLR27_06745"
     CDS             complement(505..933)
                     /locus_tag="VLR27_06745"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSP03181.1"
                     /translation="MIFLLWSLLTLPVKLVLGVVKLVLRTIGFIGLSRIAAFGAGVGV
                     GVAMAPKPAAQLRNGVAAQEEAKAARGGDIGATVRHELATSPRTWHLPQPGVTVQGDR
                     VTLTGTVPHDDARLTLIRTAGSVTGVRAVDDHLTIEPPTP"
     gene            complement(930..2153)
                     /gene="ftsY"
                     /locus_tag="VLR27_06750"
     CDS             complement(930..2153)
                     /gene="ftsY"
                     /locus_tag="VLR27_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HSP03182.1"
                     /translation="MEILLLIAAVVVLVGGVAVVLVNRRDTGAPGIEPPARTGRQVPG
                     PGTEGTPVLDGDRSGTVRDAGTSDIGIEGGTREAAEGPEGTDPVEAGGIETHHEDQPV
                     VRPTFRDRLTKARSTFSGYLGSLLSRSDITEETWEELEEALIRADVGVGLTTQLLDEL
                     RATVKAEGITEPQGLVDALKAQLTAKLEEGDRTLTYEPGNPNVWLFVGVNGVGKTTSI
                     GKVGKQQVSEGRSVMMAAGDTFRAAAAEQLETWAGRAGAEFHRGAEGGDPSSVIFDAI
                     AKAGARDIDLVLADTAGRLHTKSNLMEELSKVRRVADRDPGNVTEVLLVLDATTGQNG
                     LQQAKQFTEATQCTGVVLTKLDGSAKGGIALAIALDEEMGGLGLPIKLVGLGESADDL
                     IPFDPAAFVDALLTP"
     gene            2197..>2419
                     /locus_tag="VLR27_06755"
     CDS             2197..>2419
                     /locus_tag="VLR27_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSP03183.1"
                     /translation="MQSDSHAHPAAELQAVTQAVKRVIVGQDRVLERLRVGLLAKGHL
                     LLEGVPGLAKTLAMETLATVVGGSFARIQF"
BASE COUNT          309 a          798 c          939 g          373 t
ORIGIN      
        1 ctcgtcgtcg gcggagtaca cgggcacgcc gacctgctcg gccagcgtgc gcagctgctg
       61 gacggcggcc gggcgctgga ggtcggcgcc gacgaggagc gggttgcgac cctgggcctt
      121 gaaccagcgg gcgagcttgg ccgagttggt ggtcttgccc gagccctgga ggccggccat
      181 gagcaccacg gtgggcggct tcgatgcgta gctgatcttg atcgtctcgc cgccgaggga
      241 ctcgacgagc tcctcgttga cgatcttgat gacctgctgg gccgggttca gcgcctggct
      301 gacctcgatg cccacgaccc gctcgcggat gcggctgacc aggttcttga cgacctggaa
      361 gttgacgtcg gcctcgagca gggcgaggcg gatctcgcgg agcgtctcgt cgacgtcctc
      421 ctcgcgcagc ttccccttgc ccttgatgcg ggtgaagatg ccgtcgaacc ggtcgctgag
      481 tgcctcgaac atggggtgtc gatcctacgg ggtggggggt tcgatggtga ggtggtcgtc
      541 gacggcccgc accccggtga cggagcctgc cgtgcggatc agggtgagac gggcgtcgtc
      601 gtgggggacg gtgccggtga gggtcacccg gtcgccctgc accgtgacgc cgggctgggg
      661 caggtgccag gtgcggggtg aggtggccag ctcgtggcgc accgtggcgc cgatgtcgcc
      721 gccgcgggcg gccttggcct cctcctgggc ggcgacgccg ttgcgcagct gggcggcggg
      781 cttgggtgcc atcgccacgc ccacgccgac gccggcgccg aacgcggcga tgcggctgag
      841 gccgatgaac ccgatggtgc gcaggaccag cttcaccacg ccgagcacga gcttgaccgg
      901 cagcgtcagc agactccaga ggaggaagat cacggcgtca ggagggcgtc gacgaaggcg
      961 gccgggtcga aggggatgag gtcgtcggcg gactcgccga ggccgaccag cttgatcggc
     1021 aggccgagcc cgcccatctc ctcgtcgagg gcgatggcga gggcgatgcc gcccttggcc
     1081 gagccgtcga gcttggtgag cacgacgccg gtgcactggg tggcctcggt gaactgcttg
     1141 gcctgctgca ggccgttctg gccggtggtg gcgtcgagca cgagcaggac ctcggtgacg
     1201 ttgccggggt cgcggtcggc cacccggcgt accttcgaca gctcctccat gaggttggac
     1261 ttggtgtgga ggcgaccggc cgtgtcggcc aggacgaggt cgatgtcgcg ggcgccggcc
     1321 ttggcgatgg cgtcgaagat gaccgacgac gggtcaccgc cctcggcgcc ccggtggaac
     1381 tcggcgccgg cccggccggc ccaggtctcg agctgctcgg cggcggcggc acggaaggtg
     1441 tcgccagcgg ccatcatcac cgaccgcccc tcggacacct gctgcttgcc caccttgccg
     1501 atggaggtgg tcttgccgac gccgttgacg ccgacgaaga gccacacgtt ggggttgccc
     1561 ggctcgtagg tgagggtgcg atcgccctcc tcgagcttgg cggtgagctg ggccttgagc
     1621 gcatcgacca gaccctgggg ctcggtgatg ccctcggcct tgacggtggc gcgcagctcg
     1681 tcgagcagct gggtggtgag cccgacgccg acgtcggcgc ggatgagggc ctcctcgagc
     1741 tcctcccagg tctcctcggt gatgtcggac cgggacagga gcgagccgag gtagccggag
     1801 aaggtggagc gggccttggt gaggcggtca cggaaggtgg gccgcaccac cggctggtcc
     1861 tcgtggtggg tctcgatgcc gccggcctcc accgggtcgg tcccctccgg gccctcggcg
     1921 gcctcgcggg tgccgccctc gatgccgatg tcgctggtgc cggcgtcgcg gaccgtgccg
     1981 gagcggtcgc cgtcgagcac gggcgtgccc tcggtgccgg gtccggggac ctggcgaccg
     2041 gtccgggccg ggggctcgat gccgggtgcg ccggtgtccc tccggttgac caggacgacg
     2101 gcgacacctc cgaccaggac gacgacggcg gcgatgagca ggaggatctc catgggcgag
     2161 cgagcctatc gccgacccgc ctctagcctt cgaggggtgc agagcgactc ccacgcccat
     2221 cccgctgccg agctccaggc cgtgacccag gcggtcaagc gggtcatcgt cggccaggac
     2281 cgcgtgctcg agcgcctgcg ggtgggcctg ctggccaagg gccacctgct gctcgagggc
     2341 gtgccgggcc tggccaagac gctcgccatg gagaccctgg ccacggtcgt cgggggcagc
     2401 ttcgcccgca tccagttca
//
LOCUS       DATISU010000164         3834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_147494, whole genome shotgun sequence.
ACCESSION   DATISU010000164 DATISU010000000
VERSION     DATISU010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147494"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VLR27_06760"
     CDS             complement(<1..481)
                     /locus_tag="VLR27_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03184.1"
                     /translation="MAGEHVRDTGRTTPRGDFWAQVLPRTALGLASLLFFMAVSAAFS
                     GAALFAYYRFELDDTRERVAELEGEIADDLEFAEQAIAERTDEATAEVEGLLGELEQF
                     AASGQTVEEVLSAVGPSVFFVSTLDEAGQPSVGSAFVLFSDSERSFLLTSFETVRAAT
                     "
     gene            545..1036
                     /locus_tag="VLR27_06765"
     CDS             545..1036
                     /locus_tag="VLR27_06765"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HSP03185.1"
                     /translation="MILVLLLLLPILELFVFVQVAGSLGFLNALFGAIAATFVGIWLV
                     KQQGLGVLRRANEKVARGDAPTDELVNGTLLLVAGILLLTPGFVTGAVGLLLLLPPVR
                     ALLRGSLRRRFASGPIVIGHTSRIGGVFGPGAGFPPPRRGDVLDADSWEEPPTGPDRP
                     ELP"
     gene            1033..1788
                     /locus_tag="VLR27_06770"
     CDS             1033..1788
                     /locus_tag="VLR27_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03186.1"
                     /translation="MTVDAPFPDAGDEATVVPRPAATVMLLRDRPDGMEVLLLQRAAS
                     TPFVPGAHVFPGGAVDDGDADHDDIADGLPADAADRILGVEHGGRAFWLAAVRECVEE
                     AGVVLAIDADGTPVTDQHPVFTDLDATRDALEAGTRTLADLYREHRLRIPLDRVAYVS
                     RWITPEPSPRRYDARFFAAAMPEGQHAGADGWEAVDAGWWKPTQALADWQAGRIELIE
                     PTVVSLQLLCRFASTSEALAAIGEGAGVAEGRP"
     gene            1785..2645
                     /locus_tag="VLR27_06775"
     CDS             1785..2645
                     /locus_tag="VLR27_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSP03187.1"
                     /translation="MSAGDRDTETDEPRPIVHEVPSAVSPYIRRIVADNPGAMTGPGT
                     NTYLAGVDEIAVIDPGPDTDAHIDAVAGCGGDRIRWILLTHTHPDHAPATKRLKARTG
                     AEVCAFDDRDDVPVDRFLADGDTVETTEFRIRAIHTPGHASNHLCYLLEEERTLFSGD
                     HIMDGSTVVIAPPDGDMAAYLDSLAKVRAIKRLRAIAPGHGHLITDPIERIDAYVAHR
                     LEREAQVLGAVQDAGTTTIPDIVARLYTGLVEELVPRAEQSVHAHLQKLAGEGKVRGE
                     AADGDWTAST"
     gene            2690..>3834
                     /locus_tag="VLR27_06780"
     CDS             2690..>3834
                     /locus_tag="VLR27_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSP03188.1"
                     /translation="MAPRLISVMEAADALGLPVSGVEALADAGYLHLDQQGVSLSEIK
                     AFQARNASGAGDTAADLLGDIGAADEEAEALLDALEASVGDMADRAADIMAATFPEAA
                     GWTGQQRRRFVRQARLRFEAIVTVTRAETTDEELLEDLADAGGAAAFAGAPLPQVLLT
                     LRISRDLLVQSAVAAAAELGQQRSVALAVVLTRVLPVLDRLTDTVARGYWAAVRMREQ
                     EVLARYAHMVEHAGHGVYEVELDGTVSFANTALARICGHPLDQVLGRDLAVALPPVDP
                     ALGVDVYRVPTVSGWRPLRVLRADGVERELLLQVTERTEHGRAVGFDGIVRDVTPERQ
                     LERQKNDFLALVTDELRQPLATVLGLGVTLAAEADELPRDRIAQMGRT"
BASE COUNT          521 a         1490 c         1327 g          496 t
ORIGIN      
        1 ccgtggcggc ccgcaccgtc tcgaacgagg tgagcaggaa ggagcgctcg ctgtcggaga
       61 acagcacgaa cgcggacccg acggagggtt gcccggcttc gtcgagggtg ctcacgaaga
      121 agacggacgg cccgacagca gagagcacct cctccaccgt ctggccggaa gcggcgaact
      181 gctcgagctc gccgagcagc ccctcgacct cggctgtggc ctcgtcggtc cgctcggcga
      241 tggcctgttc ggcgaactcg aggtcgtcag cgatctcgcc ttcgagctcg gccacccgct
      301 cgcgggtgtc gtccagctcg aagcggtagt aggcgaagag cgcggcgccc gagaacgccg
      361 ccgagaccgc catgaagaac aggagcgagg cgaggccgag cgccgtgcgc ggcagcacct
      421 gggcccagaa gtcacctcga ggtgtcgtgc gcccggtgtc gcggacgtgc tccccagcca
      481 tcccggcgac ggtaccccag ccggggaacg acgccggccc ggcgtcagta gcctggaccg
      541 tcccatgatc ctcgtcctgc tgctgctcct gccgatcctc gagctcttcg tgttcgtgca
      601 ggtcgccggc tccctcggct tcctgaacgc cctgttcggc gccatcgcgg cgaccttcgt
      661 cggcatctgg ctggtcaagc agcagggtct cggcgtgctg cgccgggcca acgagaaggt
      721 cgcccgcggc gacgccccca ccgacgagct ggtgaacggc acgctgctgc tcgtcgccgg
      781 gatcctgctg ctgacgccgg gcttcgtgac cggcgccgtc ggcctgctgc tcctgctgcc
      841 gcccgtgcgg gcgctgctgc gcggctccct gcgccggcgc ttcgcctcgg gaccgatcgt
      901 gatcggtcac accagccgca tcggcggggt cttcggcccg ggggccgggt tcccaccgcc
      961 ccgtcggggt gacgtgctcg acgccgactc ctgggaggag ccaccgacgg gccccgatcg
     1021 gcctgagctg ccgtgaccgt cgacgcgccg ttccccgatg ccggtgacga ggccacggtc
     1081 gtgccgcgac cggcggcgac ggtgatgctg ctgcgcgacc gccccgacgg gatggaggtc
     1141 ctgctgctgc agcgggccgc cagcaccccc ttcgtgccgg gcgcccacgt gttccccggc
     1201 ggcgcggtgg acgacggcga cgccgaccac gacgacatcg cggacgggct cccggccgac
     1261 gccgccgacc gcatcctcgg tgtggagcac gggggccgtg cgttctggct ggccgccgtg
     1321 cgcgagtgcg tggaggaggc cggcgtggtg ctcgccatcg acgccgacgg cacccccgtc
     1381 accgaccagc acccggtctt caccgacctc gacgccaccc gcgacgcgct cgaggccggc
     1441 acccggacgc tcgccgacct ctaccgcgag caccggctgc ggatcccgct cgaccgcgtg
     1501 gcctacgtgt cgcggtggat caccccggag ccctcgcccc gccgttacga cgcccgcttc
     1561 ttcgccgccg ccatgccgga gggccagcat gccggcgccg acggctggga ggcggtcgat
     1621 gccggctggt ggaagccgac gcaagccctg gccgactggc aggctggacg gatcgagctc
     1681 atcgaaccca ccgtcgtcag cctgcagctg ctctgccggt tcgcgtccac atccgaggcc
     1741 cttgccgcca tcggcgaggg tgccggcgtg gcggagggtc ggccatgagc gccggcgacc
     1801 gggacaccga gaccgacgag ccacgcccca tcgtccacga ggtgccgtcg gccgtcagcc
     1861 cgtacatccg gcgcatcgtg gccgacaacc ccggcgcgat gaccggaccc ggcacgaaca
     1921 cctacctcgc cggggtggac gagatcgcgg tcatcgaccc cggcccggac accgacgccc
     1981 acatcgacgc ggtggccggc tgcgggggcg atcgcatccg ctggatcctg ctcacccaca
     2041 cccaccccga ccacgccccg gccaccaagc ggctgaaggc ccgcaccggc gccgaggtct
     2101 gcgcgttcga cgaccgagac gacgtgcccg tcgaccgctt cctcgctgac ggcgacacgg
     2161 tcgagaccac cgagttccgc atccgcgcca tccacacgcc tggtcacgcg tccaaccacc
     2221 tctgctacct cctcgaggag gagcggacgc tgttctccgg cgaccacatc atggacgggt
     2281 ccaccgtcgt gatcgccccg cccgacggcg acatggccgc ctacctcgac tcgctggcca
     2341 aggtccgggc catcaagcgc ctgcgggcca tcgcccccgg ccacgggcac ctcatcaccg
     2401 acccgatcga gcgcatcgac gcctacgtcg cccaccgcct cgagcgcgag gcccaggtgc
     2461 tgggcgccgt gcaggacgcc ggcaccacca cgatccccga catcgtcgcc cggctctaca
     2521 ccgggctggt ggaggagctc gtgccccggg ccgagcagtc cgtgcacgcc cacctccaga
     2581 agctggctgg cgagggcaag gtgcgcggcg aggccgccga cggcgactgg accgcctcga
     2641 cctgacatca cggagggatc gctccaccct ccgtcgaagc aaggaccgga tggctccccg
     2701 cctgatctcc gtgatggagg ccgccgatgc cctcgggctc cccgtctcgg gcgtcgaggc
     2761 gctggccgac gcgggctacc tccacttgga ccagcagggt gtgtcgctgt ccgagatcaa
     2821 ggcgttccag gcccgcaacg ccagcggtgc tggcgacacc gccgccgacc tgctggggga
     2881 catcggcgcg gccgacgagg aggccgaggc gctcctcgac gcgctcgagg cgtcggtcgg
     2941 cgacatggcc gaccgagccg cggacatcat ggcggcgacg ttcccggagg ccgccggctg
     3001 gaccggccag cagcgccggc ggttcgtgcg ccaggcccgg ctgcggttcg aggccatcgt
     3061 caccgtcacc cgggccgaga ccaccgacga ggagctgctc gaggacctgg ccgacgcggg
     3121 aggcgccgct gccttcgccg gcgcacccct gccccaggtg ctgctgaccc tgcgcatcag
     3181 ccgggacctg ctggtgcaga gcgccgtcgc cgcggccgcc gagctgggcc agcagcgcag
     3241 cgtcgccctc gccgtcgtgc tcacccgggt gctgccggtg ctcgaccgac tcaccgacac
     3301 cgtcgctcgc ggctactggg ccgcggtccg catgcgggag caggaggtcc tcgcccggta
     3361 cgcccacatg gttgagcacg ccggccacgg cgtctacgag gtcgagctcg acggcaccgt
     3421 gtcgttcgcc aacaccgccc tcgcccgcat ctgcgggcac cccctcgacc aggtgctcgg
     3481 tcgggacctc gcggtggcgc tgccgccggt cgacccggcg ctcggcgtcg acgtgtaccg
     3541 cgtgcccacg gtcagcgggt ggcgccccct ccgggtcctg cgcgccgacg gggtcgagcg
     3601 ggagctgctg ctccaggtca ccgagcgcac cgagcacggc cgggccgtgg gcttcgacgg
     3661 gatcgtgcgc gacgtcaccc cggagcgcca gctcgagcgg cagaagaacg acttcctcgc
     3721 cctggtcacc gacgagctgc gccagccgct ggccaccgtg ctcggcctcg gggtcaccct
     3781 ggccgccgag gccgacgagc tcccccgcga ccgcatcgcc cagatggggc gcac
//
LOCUS       DATISU010000165         7974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_706188, whole genome shotgun sequence.
ACCESSION   DATISU010000165 DATISU010000000
VERSION     DATISU010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706188"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VLR27_06785"
     CDS             <1..325
                     /locus_tag="VLR27_06785"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HSP03189.1"
                     /translation="SVEAIRAGGDDAKLIWVDTDGCESVADACDLFLTSVMKNFDVSV
                     YDAVTAAANGEFAGGVYSGTLENDGVELGPFNEFEGDISDELQSELDEIRAGIIDGSI
                     ATTLE"
     gene            438..1943
                     /locus_tag="VLR27_06790"
     CDS             438..1943
                     /locus_tag="VLR27_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP03190.1"
                     /translation="MRVELRGITKRFPGVVANEDVNLVVEPGQVHALLGENGAGKSTL
                     MNVLFGLYRQTEGEVLLDGEPASFSSPADAIDAGIGMVHQHFMLVPVFTVAENVMLGV
                     EPTSAPGVLDRDAARRRVLELSARHGLDVDPDAMVEDLPVGVQQRVEILKALYREADC
                     LILDEPTAVLTPTEIDDMLGIIRRLADEGRSVIFISHKLREVRAIADTITILRRGRVV
                     GTTTPDESDEQELATAMVGRDVQLVVDRSAASPTDVVLRVSDLRVRDDRDQLAVADVS
                     FDVRAGEIVAIAGVQGNGQSELVSGIVGLRRPTAGRVELDGTDVTGANPARMVELGVA
                     HVPEDRQRDGLISSFPVSDNLVLNLTGRAPYSRGIRMDRRAIRENAERLVQDFDIRTS
                     SIDAAVGTLSGGNQQKVIVGREMSHTERLLVLSQPTRGLDVGSIQYIHHRIVQARDQG
                     LAVLLNSSELDEVLALADRVIVMYRGGVIGEVSGDEATRDSVGMLMAGARA"
     gene            1940..3184
                     /locus_tag="VLR27_06795"
     CDS             1940..3184
                     /locus_tag="VLR27_06795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP03191.1"
                     /translation="MTAPPKEAAPEALPPEADDPLAGAPHDRRPGVLGALREVALGLI
                     GPLLAILTALIVGALIIVFTDPTTLEAWGSFFSAPGTALSDSWDLIATSYRALFDSSL
                     GSQRALARTLVQSTPLILVGLSVALAFKAGMFNIGGAGQLIVGAMAAAWIGITFDLPQ
                     IVHLPLALVAGIIGGAFWGGIVGFLKARTGAHEVITSIMLNFVALRLLDYALSTDTFR
                     RPGRSDPISPEMPASVRLPVPIDSFRVHLGFVLALLVAWGAWWLLQRTTIGFRMRAVG
                     ANAKAARYAGMGVAATTVLSMGIAGGLAGLGGTTILLGSTPRLTGGFFASVGFDAIAL
                     ALLGRASPIGVVGAGLLFGALQAGSTGMQAQTSTPIDIIFVIQALIILFVAAPAIVRT
                     VWRVKETRLGAGQRFSAGWGAQ"
     gene            3190..4467
                     /locus_tag="VLR27_06800"
     CDS             3190..4467
                     /locus_tag="VLR27_06800"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP03192.1"
                     /translation="MTTAPAPAPRDDVVEDTGARRHALTVAGLCIAAGLAAVVLFGLT
                     ATSDQVSTMLMNPRGVDTIPDIELPTQIAAFVLGFGAIALGVRALQVADPKITVFFGG
                     ALGLFALALVIWAARDGTGAFYGLIAGSVQRATPIALGAIAGLLCERTGVINIAIEGM
                     MLAAAFGAAITASATGSLWIGLLCGILTGALFGAIHAVISIRYEVDQIVGGTFINIFA
                     LGLTSYLTVRVLVEYSHLNQPGTFRPFEIPLLVEIPFLGPLLFSQNIFGFIMFGLVAF
                     LAWALFNTRWGLRVRSVGEHPRAADTVGISVRFIRYRNVILGGMVAGLGGAWFTLGSA
                     GRFDQNMTNGKGFIALAAMIFGRWHPVGALIAALIFGFSEQLNDYLALLDTPIPSEFL
                     LMAPYVVTIVVVAGLVGRPRVPAANGQVYRSGG"
     gene            4464..4850
                     /locus_tag="VLR27_06805"
     CDS             4464..4850
                     /locus_tag="VLR27_06805"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HSP03193.1"
                     /translation="MSPTPDWVALRAAAAEAARRSYSPYSGFPVGAAALLRDGATISA
                     ANVENASYTVGICAETALVGVLVAGGHERGDLVAVAVTDPDGNDLSACGRCRQLLHEM
                     GGGPMPLNERPMDEWLPDAFGPEDLT"
     gene            4850..6124
                     /locus_tag="VLR27_06810"
     CDS             4850..6124
                     /locus_tag="VLR27_06810"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HSP03194.1"
                     /translation="MATDILDVLRTKRDGGRLSDEQIRFFIDGYTAGTIADEQAAALL
                     MAIVWRGMEPDELATWTAAMIDTGERADLSFLDRPTVDKHSTGGVGDKISLILVPLVA
                     ACGAAVPQLAGRGLGHTGGTLDKLEAIAGFQSVLPADRYRQVLADVGGVIAGASGDIA
                     PADRKLYALRDVTATVESIPLIASSIMSKKIAEGTRGLVLDVKLGSGSFLGDDAATRV
                     LAETMVGIGNGNGVDTVALLTDMDQPLGRAAGNALEVRESLDVLAGGGPADVRELTLA
                     LAREMLGLAGIDTDPEPVLDDGRAMEVFERIVAAQGGDLTVPLPTSEHTHVVTASRDG
                     WLQRMDCKAVGVAVWRLGAGRARKEDAVSFGAGALVEAKVGDRVAAGDPVLVLHADDP
                     DRFAGALAALEGAIDIGDERVAPRPLVADRIT"
     gene            complement(6201..6584)
                     /locus_tag="VLR27_06815"
     CDS             complement(6201..6584)
                     /locus_tag="VLR27_06815"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HSP03195.1"
                     /translation="MPARSESRFVAVVRFAFRSSKQIAVTVVGAALVLAGIAMLVLPG
                     PGILVVVAGFAVLGTEYAWAAAALDRTKATAERAGQVAKGAAGSSVRVAKGAAVSTGR
                     AARGAAGGAAGRLRRRRGSGGVGDV"
     gene            6624..7715
                     /locus_tag="VLR27_06820"
     CDS             6624..7715
                     /locus_tag="VLR27_06820"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HSP03196.1"
                     /translation="MPHRPTLELIRQAPKVLLHDHLDGGLRPTTVIELAAEQGYTDLP
                     TTDPDELAAWFVEGAARRSLPRYLEPFQHTVGVMQTPEALTRVAAECAEDLADDGVVY
                     AEVRFAPELHLDGGMELDQIVESVLAGFEQVTSQGRIQIGLLLTAMRTAANSSEIAEL
                     TLRHRDAGVVGFDIAGSEAGYPPTRHLDAFRTIAAANHHITIHAGEAFGLPSIWEAIQ
                     ICGAERLGHGVRIVDDIEVQPDGSVKLGELASFVRDRRVPLEMCPTSNVHTGVVDDLS
                     EHPIDLLTRLRFRVTVNTDNRLMSDVTMSSEMYALCQTFGYGWTDLQWFTVNAMKSSF
                     WPFDKRLRIINEIIKPGYASLQARSLESA"
     gene            7738..>7974
                     /locus_tag="VLR27_06825"
     CDS             7738..>7974
                     /locus_tag="VLR27_06825"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSP03197.1"
                     /translation="MADRIEITRDIAASPEAVYAAISNVTRMGEWSEECHACEWHEGV
                     DGPEVGATFDGHNRNGEHEWTSQGKVIEAEPGRSF"
BASE COUNT         1101 a         3026 c         2692 g         1155 t
ORIGIN      
        1 ctccgtcgag gcgatccgcg ccggcggcga cgacgccaag ctgatctggg tcgacaccga
       61 cggctgcgag tccgtcgccg acgcctgcga cctgttcctg accagcgtca tgaagaactt
      121 cgacgtgtcg gtctacgacg cagtgacggc tgcggccaac ggcgagttcg ccggcggcgt
      181 ctacagcggc acgctcgaga acgacggcgt ggagctcggc ccattcaacg agttcgaggg
      241 cgacatctcc gacgagctcc agagcgagct ggacgagatc cgggccggca tcatcgacgg
      301 ctcgatcgcc acgacgctcg agtagcgcac ggcaccgccc gcttcgggcg atttgccgta
      361 gaagtgagcc agaggccggg tttcgcaccc ggcctctggc gcgtccggag ccccggcacg
      421 cgctagacat cccggcgatg cgggtcgagc tgcgtgggat caccaagcgc ttccccggcg
      481 tggtcgccaa cgaagacgtg aacctggtgg tcgagcccgg ccaggtgcat gcgctcctcg
      541 gcgagaacgg cgccgggaag tccaccctga tgaacgtgct gttcgggctg taccgccaga
      601 ccgagggcga ggtgctcctc gacggcgagc cggcgtcgtt ctcgtccccc gccgacgcca
      661 tcgacgccgg catcggcatg gtgcaccagc acttcatgct cgtccccgtg ttcacggtcg
      721 ccgagaacgt catgctcggc gtggagccga cctcggcgcc cggcgtgctc gaccgcgacg
      781 ccgcccggcg ccgggtgctc gagctctcgg cccgccacgg cctcgacgtg gaccccgatg
      841 cgatggtgga ggacctgccc gtcggcgtgc agcagcgggt ggagatcctc aaggcgctct
      901 accgcgaggc cgactgcctc atcctcgacg agcccaccgc cgtcctcacg ccgaccgaga
      961 tcgacgacat gctcgggatc atccgccgcc tggccgacga gggccgctcg gtgatcttca
     1021 tctcccacaa gctgcgggag gttcgggcca tcgccgacac catcacgatc ctgcgccgcg
     1081 ggcgggtggt cggcaccacc acccccgacg agagcgacga gcaggagctg gccaccgcca
     1141 tggtcggtcg cgacgtgcag ctcgtggtcg atcgcagcgc tgcctccccc accgatgtgg
     1201 tgctgcgggt ctcggacctg cgcgtgcgcg acgaccgcga ccagctggcc gtggccgatg
     1261 tgtccttcga cgtgcgggcc ggggagatcg tggccatcgc cggcgtgcag ggcaacggcc
     1321 agtccgagct ggtgtcgggc atcgtcgggc tgcgccggcc cacggccggt cgggtggagc
     1381 tggacggcac cgacgtcacc ggtgcgaacc cggcccgcat ggtggagctc ggcgtggccc
     1441 acgtgcccga ggaccgccag cgcgacggcc tcatctcctc gttcccggtg tcggacaacc
     1501 tggtgctgaa cctcacggga cgggcgccgt actcacgggg catccgcatg gaccgccggg
     1561 ccatccgcga gaacgccgag cgcctggtcc aggacttcga catccggacc tcgtccatcg
     1621 atgccgccgt cggcaccctg tcgggcggca accagcagaa ggtgatcgtg ggacgggaga
     1681 tgtcgcacac cgagcggctg ctcgtgctga gccagccgac ccgtggcctc gacgtcgggt
     1741 cgatccagta catccaccac cgcatcgtcc aggcccgcga ccagggcctc gccgtcctcc
     1801 tcaactcctc cgagctcgac gaggtgctcg ccctcgccga ccgggtgatc gtgatgtacc
     1861 gcggcggcgt gatcggtgag gtcagcggcg acgaggccac ccgcgactcg gtcggcatgc
     1921 tcatggccgg agcgcgggca tgaccgcccc gccgaaggaa gccgcgcccg aggccctgcc
     1981 gcccgaagcg gacgatcccc tcgccggcgc accccacgac cggcgcccgg gcgtcctcgg
     2041 cgccctgcga gaggtcgccc tcggtctgat cggcccgctc ctggcgatcc tcaccgcgct
     2101 catcgtcggt gcgctcatca tcgtcttcac cgacccgacc acgctcgagg cgtgggggtc
     2161 gttcttctcg gcgcccggca ccgcgctgtc ggactcctgg gacctcatcg ccaccagcta
     2221 ccgggccctc ttcgactcct cgctcggcag ccagcgggcc ctggcccgca cgctcgtgca
     2281 gtccaccccg ctgatcctcg tgggcctctc cgtggcgctc gccttcaagg ccgggatgtt
     2341 caacatcggc ggcgcaggcc agctgatcgt gggcgcgatg gccgccgcct ggatcgggat
     2401 caccttcgac ctcccccaga tcgtgcacct cccgctcgcc ctggtcgccg ggatcatcgg
     2461 cggcgccttc tggggcggga tcgtcgggtt cctcaaggcg cgcaccggcg cccacgaggt
     2521 gatcacctcg atcatgctga acttcgtggc cctgcggctg ctcgactacg cgctgtccac
     2581 cgacacgttc cgccgcccgg gccgcagcga cccgatcagc ccggagatgc cggcctcggt
     2641 ccgcctgccg gtgccgatcg actcgttccg ggtccacctc gggttcgtgc tcgccctgct
     2701 cgtggcctgg ggcgcgtggt ggctgctgca gcgcaccacg atcggcttcc gcatgcgggc
     2761 cgtgggggcc aacgccaagg cggctcgcta cgccggcatg ggcgtggcgg ccaccaccgt
     2821 gctgtcgatg ggcatcgccg gcggcctcgc cggcctcggt ggcaccacga tcctgctggg
     2881 cagcacgccc cgactcacgg gtggcttctt cgccagcgtc ggcttcgacg ccatcgcgct
     2941 cgccctgctc ggccgagcga gccccatcgg cgtcgtcggc gccggcctgc tcttcggcgc
     3001 cctccaggcc gggtccaccg gcatgcaggc ccagacctcc acgcccatcg acatcatctt
     3061 cgtcatccag gcgctgatca tcctgttcgt cgccgcgccg gccatcgtgc ggacggtctg
     3121 gcgggtcaag gagacccgcc tcggcgccgg gcagcggttc agcgccggct ggggagccca
     3181 atgacctccg tgaccaccgc ccccgcgccc gcaccccgcg acgacgtcgt cgaggacacc
     3241 ggcgcccgtc gtcacgccct caccgtcgcc ggcctgtgca tcgccgcggg cctcgcagcc
     3301 gtcgtgctgt tcggcctcac cgccacatcg gaccaggtgt ccacgatgct gatgaacccc
     3361 cgcggcgtcg acacgatccc cgacatcgag ctgccgaccc agatcgctgc gttcgtcctc
     3421 gggttcggcg ccatcgcgct cggtgtgcgg gcgctgcagg tggccgaccc caagatcacg
     3481 gtgttcttcg gcggtgcgct cggcctgttc gccctcgccc tcgtcatctg ggccgcccgc
     3541 gacggcaccg gcgccttcta cggcctcatc gccggttcgg tccagcgggc gacgcccatc
     3601 gccctcgggg ccatcgccgg cctgctgtgc gagcggaccg gcgtcatcaa catcgccatc
     3661 gagggcatga tgctggccgc cgcgttcggc gccgccatca ccgcgagcgc caccgggagc
     3721 ctctggatcg gcctgctgtg cggcatcctc accggcgccc tgttcggggc gatccacgcc
     3781 gtcatcagca tccgctacga ggtcgaccag atcgtcggcg gcacgttcat caacatcttc
     3841 gcgctcgggc tcaccagcta cctgaccgtg cgcgtcctgg tggagtactc ccacctcaac
     3901 cagcccggca cgttccggcc cttcgagatc ccgctgctgg tggagatccc cttcctcgga
     3961 ccgctcctgt tcagccagaa catcttcggg ttcatcatgt tcggcctggt ggcgttcctg
     4021 gcctgggcgc tgttcaacac ccggtggggt ctgcgggtcc gctcggtcgg cgagcacccc
     4081 cgcgctgccg acacggtcgg gatcagcgtg cggttcatcc gctaccgcaa cgtgatcctc
     4141 ggcggcatgg tggccggcct cggtggtgcc tggttcacgc tcggctcggc cggccgcttc
     4201 gaccagaaca tgaccaacgg caagggcttc atcgccctcg ccgccatgat cttcggtcgc
     4261 tggcacccgg tcggcgccct catcgccgcg ctgatcttcg gcttctccga gcagctcaac
     4321 gactacctcg ccctgctcga cacgcccatc ccgtcggagt tcctgctcat ggccccctac
     4381 gtggtgacca tcgtcgtggt ggccggcctg gtcgggcggc cccgggtccc ggcggccaac
     4441 ggccaggtgt accggtccgg cggatgagcc cgaccccgga ctgggtcgcc ctgcgggcgg
     4501 ccgcggccga ggcggcccgg cgctcgtact ccccctactc ggggttcccg gtcggcgctg
     4561 cggcgctgct gcgcgacggt gccacgatca gcgccgccaa cgtcgagaac gccagctaca
     4621 ccgtgggcat ctgcgccgag accgccctgg tcggcgtcct cgtggccggg ggacacgagc
     4681 ggggcgacct cgtggcggtc gcggtcaccg accccgacgg caacgacctg tcggcctgcg
     4741 ggcgctgccg ccagctgctg cacgagatgg gtggcggtcc gatgcccctc aacgagcgac
     4801 ccatggacga gtggctgccc gacgccttcg gtccggagga cctgacctga tggccaccga
     4861 catcctcgac gtgctgcgca ccaagcgcga cggcggccgg ctcagcgacg agcagatccg
     4921 cttcttcatc gacggctaca cggccggcac catcgccgac gagcaggcgg ccgcgctgct
     4981 gatggccatc gtgtggcggg gcatggagcc cgacgagctg gccacatgga cggctgcgat
     5041 gatcgacacc ggcgagcgcg ccgacctgtc gttcctcgac cggcccacgg tggacaagca
     5101 ctccaccggc ggcgtcggcg acaagatctc cctgatcctg gtccccctcg tggccgcctg
     5161 cggggccgcc gtgccccagc tggccggccg gggcctcggc cacaccggtg gcacgctcga
     5221 caagctcgag gccatcgccg gcttccagtc ggtgctccca gcagaccgct accgccaggt
     5281 cctcgccgac gtgggcgggg tgatcgccgg cgccagcggc gacatcgctc cggccgaccg
     5341 caagctctac gccctgcgcg acgtcaccgc caccgtcgag tcgatcccgc tcatcgccag
     5401 ctcgatcatg tcgaagaaga tcgccgaggg cacccgcggc ctggtgctcg acgtgaagct
     5461 cgggtcgggc tcgtttctcg gcgacgacgc cgccacccgg gtgctggccg agaccatggt
     5521 cggcatcggc aacgggaacg gcgtcgacac ggtcgcgctg ctcaccgaca tggaccagcc
     5581 cctcggccgg gccgcgggca acgcgctcga ggtgcgggag tccctcgacg tgctggccgg
     5641 cggcggtccc gccgacgtgc gcgagctcac cctggcgctg gcccgggaga tgctcggcct
     5701 cgccgggatc gacaccgacc ccgagcccgt gctcgacgac ggccgggcca tggaggtctt
     5761 cgagcgcatc gtggcggccc agggcggcga cctgaccgtg ccgctgccca cgtcggagca
     5821 cacccacgtg gtcaccgcgt cccgggacgg gtggttgcag cgcatggact gcaaggccgt
     5881 cggcgtcgcc gtgtggcgcc tcggggccgg gcgagcccgc aaggaggacg ccgtgtcctt
     5941 cggtgccggc gccctggtgg aggccaaggt cggcgaccgc gtcgccgccg gcgaccccgt
     6001 gctcgtgctc cacgccgacg accccgaccg cttcgccggt gcgctggccg ccctggaggg
     6061 cgccatcgac atcggcgacg agcgggtggc accccgcccc ctcgtcgccg accgcatcac
     6121 ctgacgtcac cctgcaccgt tctggagggc tctgcacgcc tccgaggcgt gccccgcggc
     6181 cagaacgatc tgggtggggc tcagacgtcg ccgacgccgc ctgagccacg acggcgacgg
     6241 aggcggccgg cggcaccacc ggccgcgcct cgtgccgccc ggccggtgct gacggcggcg
     6301 cccttggcga cccggaccga cgagcccgcc gcacccttgg ccacctggcc ggcgcgctcc
     6361 gcggtggcct tggtgcggtc gagggcggca gccgcccagg cgtactcggt gccgagcacg
     6421 gcgaagccgg ccaccacgac caagatcccg gggccgggca gcacgagcat cgcgatgccg
     6481 gcgagcacga gggcggcccc gaccacggtg accgcgatct gcttcgagga gcggaaggcg
     6541 aagcggacga cggcgacgaa gcgggactcg gaacgggctg gcatcgaccg atcgtacggc
     6601 gaggcaaccg gtagcgtcag cgcatgccgc accgccccac gctcgagctg atccggcaag
     6661 cccccaaggt cctcctgcac gaccacctcg acggggggct gcgcccgacg acggtgatcg
     6721 agctggccgc cgagcagggc tacacggacc tgcccaccac ggaccccgac gagctggcgg
     6781 cctggttcgt cgagggcgcc gctcggcgca gcctgccccg ctacctcgag ccgttccagc
     6841 acacggtcgg cgtcatgcag accccggagg cgctcacccg cgtcgccgcc gagtgcgccg
     6901 aggacctcgc cgacgacggc gtggtctacg ccgaggtccg cttcgcaccc gagctgcacc
     6961 tcgacggcgg catggagctg gaccagatcg tggagtccgt gctcgccggc ttcgagcagg
     7021 tgacctcgca gggccgcatc cagatcgggc tgctgctcac cgcgatgcgc acggcggcca
     7081 actcctcgga gatcgccgag ctcacactgc gccaccggga cgccggggtg gtcggcttcg
     7141 acatcgccgg cagcgaggcc ggctacccgc ccacgcgcca cctcgacgcg ttccgcacca
     7201 tcgcggcggc aaaccaccac atcaccatcc acgccggcga ggcgttcggc ctgccctcca
     7261 tctgggaggc catccagatc tgcggcgccg agcgcctcgg gcacggcgtg cgcatcgtcg
     7321 acgacatcga ggtccaaccc gacggctcgg tgaagctggg cgagctggcg tcgttcgtcc
     7381 gggaccggcg ggtgccgctc gagatgtgcc cgacctcgaa cgtgcacacg ggcgtggtcg
     7441 acgacctgtc cgagcacccc atcgacctgc tcacccgcct gcgcttccgg gtcaccgtca
     7501 acaccgacaa ccgcctcatg agcgacgtga ccatgagcag cgagatgtac gcgctgtgcc
     7561 agacgttcgg ctacggctgg accgacctgc agtggttcac cgtcaacgcc atgaagagct
     7621 cgttctggcc cttcgacaag cgcctgcgga tcatcaacga gatcatcaag cccggctacg
     7681 catcgctcca ggcccggtcg ctggaatccg cgtgatcatg tgatggactc tcgtcccatg
     7741 gccgacagga tcgagatcac ccgtgacatc gccgcctccc ccgaggcggt gtacgccgca
     7801 atctccaacg tcacccgcat gggcgagtgg tctgaggagt gccacgcgtg cgagtggcac
     7861 gagggcgtcg acgggcccga ggtgggcgcc accttcgacg gccacaaccg caacggcgag
     7921 cacgagtgga ccagccaggg caaggtgatc gaggccgagc ccggccggtc cttc
//
LOCUS       DATISU010000166         8209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_7797, whole genome shotgun sequence.
ACCESSION   DATISU010000166 DATISU010000000
VERSION     DATISU010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7797"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..2060)
                     /locus_tag="VLR27_06830"
     CDS             complement(114..2060)
                     /locus_tag="VLR27_06830"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HSP03198.1"
                     /translation="MATRTPSLGAIVIDQLRPSTPGGGGRPKTSVGETTPVQARIFRD
                     GHDLLAARVSWRKAGARKWNRSTLAQSENDIWIGSLECTDIGPHDVVVEAWTDVFATW
                     RKELRLKVGADQDVTTELEEGARILERFVPECSGKGRSRVEDAVAAIRDDTASMDVRL
                     NAALDDALVPLVAGIPHEGDLTVSDTIALWVDRERARHSAWYEFFPRSEGGFKGSLDR
                     LDAVAEMGFDVVYFPPIHPIGTTARKGPNNTLTPGPDDPGSPWAIGGPEGGHTAIHPD
                     LGSEEELLAVVQKAQDLGMEIALDYALQCSPDHPWVTEHPEWFHHRPDGSIKYAENPP
                     KKYQDIYPINFWPEDEADRVALWEACRDILVHWIDRGIRIFRVDNPHTKPVAFWEWCI
                     ADIQADHPDVLFLAEAFTRPAMMSKLAEIGFSQSYTYFTWRHEKVELTEYVEQLAHGP
                     LADVMRPNFWPNTPDILGGVLRHGNRAAFLLRYLLASTLTPNFGVYSGYELRENEPAA
                     DTNEEYLYSEKYEVKSRDWDRPDSLAPFMTKINRIRRAHPALQQLREIHFHHVDNPQV
                     IAYTKGSRASGDLVLVIVNLDPVHTQEATVHLDLHAVGLPEWEPYRAHDELTGESYEW
                     QGGANYVRFEPALDRVGHVLDLSA"
     gene            complement(2109..2768)
                     /locus_tag="VLR27_06835"
     CDS             complement(2109..2768)
                     /locus_tag="VLR27_06835"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HSP03199.1"
                     /translation="MHAAGEDGWDALDAVWQEAFRQAWEALVTGNIGVGAVVSDSDGT
                     IVAVGRNRVRDDRAPRGQVFGTTLAHAEINVLAQLPFGAPRGLVLSTTLQPCLQCAAA
                     IRMAPVARVRIAGPDPLWDGAGAFSHLNAWLGRRSDAPVEGPRRDEVGVFAVLLARLG
                     AGSRAHVDAGLRERGHGAVIDLADRLRDDGTLDRLRDGSVEDAYADLRDQLRRVGSEM
                     G"
     gene            2862..5387
                     /gene="glgP"
                     /locus_tag="VLR27_06840"
     CDS             2862..5387
                     /gene="glgP"
                     /locus_tag="VLR27_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HSP03200.1"
                     /translation="MKALRSFTVRPTLPAELAPLEALAMNLRWSWDVQTRELFRWVDP
                     DQWDAVVHDPVRLLGSVSRSRLDELVQDKGFLRFLETVSSDLERYLSRDAWFQGRTDS
                     PLRSVAYFSPEFGISEAVPQYSGGLGVLAGDHLKAASDLGLPLIGIGLMYRNGYFRQA
                     LNVEGWQEQRYPNLDPYGMALTLQEGVRVSVDLGGRALTAQVWKAEVGRTDLYLLDSD
                     VDDNDDDLRSVTDRLYGGDSEHRLRQEMLLGIGGVRALRALGRDTQVFHTNEGHAGFL
                     GLERIRELMADGLSFEQAIEAVRGGSVFTTHTPVPAGIDRFPRELIEKYFTGFAADAG
                     ITMDQLLALGHEPGDAGQTRFNMAVMGFRLAARANGVAQLHGEVSREMFQPLWPEVPV
                     EEVPIGAITNGVHARTWVSSEMDDLLTRHVHPHWGEAGTHEWDRIHDATDNDIWRVKE
                     QGKDRLVALVRDKIRSSAVARGQAPSDVAWADEVFDSRALTIGFARRFATYKRATLLL
                     SQRERLEAILGSTERPVQFVFAGKAHPADDQGKEMIRQIVAYSSDPSIRHRFVFLDDY
                     DIAIARTMYHGCDVWLNTPRRPQEACGTSGMKAALNGQLNLSILDGWWDECYDGENGW
                     AISSAENVTDTARRDEIEANSLFDLLEREVIPLYFDRSGGRLPRRWVRRMKTTLSGLG
                     PFLSASRMVKDYTTALYEPAAAHADDLTGDDHRRAKEITTWKERIVAGWHDVRVASVE
                     SDGTVPGIGDERTVDAVVSLGALAGSDVEVQLVHGIVGQGDEIEHPLTTTMSEVEPLD
                     DGQVRYRGSFACDTAGRYGFTVRVLPVHEDLAASAEMGRVAWA"
     gene            complement(5384..6226)
                     /locus_tag="VLR27_06845"
     CDS             complement(5384..6226)
                     /locus_tag="VLR27_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSP03201.1"
                     /translation="MTPLHFLVPVDPTHPAMSAAQIAVSLATPATTVELVAVTTAGLP
                     AEVVDTFLAEEQARFPGASVEVRRLEGPGVVDVLLHDIASTDPDLVVLDSHGRGPLGE
                     LVLGSVSADLVRSSSAPTLLIGPACEPVGELRRLTVAVDGSSDSLGALDVAAALSRRF
                     AVPIELVEVTEEMAYTSDVAESAELHRLALRVEPPVRSWDVLHGHDVPRALIDHVRFE
                     PGAVLVLGSHGRSAGRPNVLGGTAARTVRHSTVPVLVVSPQAAADVGRRLTERPRAEG
                     APAG"
     gene            complement(6223..6654)
                     /locus_tag="VLR27_06850"
     CDS             complement(6223..6654)
                     /locus_tag="VLR27_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSP03202.1"
                     /translation="MERIVVGVDGSDGSVAALRWGVAEAVLRGAVVEAVSAFHVPYVG
                     AASVMPLMLDPAEFSDAADAVLRKVIDQVDAGALAEPIRPLVIEGPASTVLVEAGRGA
                     SLLVVGARGHGGLAGMLLGSVSRQVTEHATVPVVVVPKERS"
     gene            complement(6766..7539)
                     /locus_tag="VLR27_06855"
     CDS             complement(6766..7539)
                     /locus_tag="VLR27_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HSP03203.1"
                     /translation="MADLVHLERRDDGVAVITLDNPKVNALSSALLHQLRDAARSLID
                     DPPRSVLVTGGPKVFAAGADISEFGGPEEAETVGGAFLDALEAVAAIPRATVAAIGGY
                     ALGGGCELALACDLRIASDRARFGQPEILLGIIPGGGGTQRLARLVGPSRAKDLIFSG
                     RQVDAEEALRIGLVDRVVPADGFEDAAMAWASQLATGPGAVVGLAKQAIDDGLDGDLA
                     DGLRLEQRLFADSFRTEDSQIGVASFREHGPGKAAFTGR"
     gene            complement(7655..>8209)
                     /locus_tag="VLR27_06860"
     CDS             complement(7655..>8209)
                     /locus_tag="VLR27_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HSP03204.1"
                     /translation="DAAGYPWVLVETVGVGQVEVEIVGAADTTVVVVNPGWGDAIQAN
                     KAGLMEIADIFVINKADRPGADETRRDLEGMLDLTGAGSEHGGAPWRPPVLSAVATEG
                     TGIAELWDQVLAHRAHLESSGELQVRRGRRAREELTRIVAERLLARARAASDGDRFED
                     LLDDVEQRGTDPWAAADELLGTPD"
BASE COUNT         1163 a         2890 c         3019 g         1137 t
ORIGIN      
        1 gccgcggagg tcgccggtgc cgtcgtggtt ggagtcgttg aacccgcgga cgagcacctc
       61 gtagaagacg gcccgctgga accagctgtg gtcggtgggg tcgatggcct tcctcatgcg
      121 gagaggtcca gcacgtggcc gacgcggtcg agggcgggct cgaagcgcac gtagttggcg
      181 cccccctgcc actcgtagga ctcgccggtg agctcgtcgt gggcccggta cggctcccac
      241 tccggcagcc ccacggcgtg caggtcgagg tgcacggtgg cctcctgggt gtggacgggg
      301 tcgaggttga cgatgacgag caccaggtcg ccggaggctc gggagccctt ggtgtaggcg
      361 atgacctggg ggttgtcgac gtggtggaag tggatctctc gcagctgctg cagggcgggg
      421 tgggcccggc ggatccggtt gatcttggtc atgaacgggg cgagggagtc cggccggtcc
      481 cagtcgcggg acttgacctc gtacttctcc gagtacaggt actcctcgtt ggtgtcggcg
      541 gccggctcgt tctctcgcag ctcgtagccg gagtacacgc cgaagttcgg ggtgagcgtg
      601 gaggccagga ggtagcgcag caggaaggcg gcgcggttcc cgtggcgcag caccccgccg
      661 aggatgtcgg gggtgttggg ccagaagttg ggccgcatga catccgcgag ggggccgtgg
      721 gcgagctgct ccacgtactc ggtgagctcc accttctcgt ggcgccaggt gaagtacgtg
      781 tagctctggc tgaacccgat ctcggccagc ttggacatca tcgccgggcg ggtgaacgcc
      841 tcggcgagga agagcacgtc ggggtggtcg gcctggatgt cggcgatgca ccactcccag
      901 aacgccaccg gcttggtgtg ggggttgtcg acgcggaaga tccggatgcc gcggtcgatc
      961 cagtgaacga ggatgtcccg gcacgcctcc cacagcgcca cccgatcggc ctcgtcctcg
     1021 ggccagaagt tgatcgggta gatgtcctgg tacttcttgg gcgggttctc ggcgtacttg
     1081 atggagccgt cgggccggtg gtggaaccac tcggggtgct cggtgaccca ggggtggtcc
     1141 ggggagcact gcagcgcgta gtccagggcg atctccatgc cgaggtcctg ggccttctgg
     1201 acgacggcca gcagctcctc ctcggagccg aggtcggggt ggatcgcggt gtgcccaccc
     1261 tcgggcccgc cgatggccca cgggctgccc ggatcgtccg ggccgggcgt gagggtgttg
     1321 ttcggaccct tccgggccgt ggtgccgatg gggtggatcg gtgggaagta gacgacgtcg
     1381 aagcccatct cggccacggc gtcgagccgg tcgagggagc ccttgaaccc gccctcggag
     1441 cgagggaaga actcgtacca ggcgctgtgg cgggcccgct cgcgatcgac ccacagggcg
     1501 atggtgtcgc tgaccgtgag gtcgccctcg tgggggatgc cggcgaccag cgggacgagc
     1561 gcgtcgtcga gggcggcgtt cagccggacg tccatggagg cggtgtcgtc ccggatcgcc
     1621 gccactgcgt cctcgacccg gctgcgaccc ttgccggagc actcggggac gaagcgctcg
     1681 aggatgcggg cgccctcctc gagctcggtc gtgacgtcct ggtcggcgcc gaccttcagg
     1741 cgcagctcct tgcgccaggt cgcgaacacg tcggtccagg cctcgaccac gacgtcgtgg
     1801 ggaccgatgt cggtgcactc gaggctgccg atccagatgt cgttctcgga ctgggccagc
     1861 gtcgagcggt tccacttgcg ggcccccgcc ttgcgccagc tgacgcgtgc ggccagcaga
     1921 tcgtggccgt cacggaagat ccgcgcctgc accggcgtgg tctcgccgac ggaggtcttg
     1981 ggtcgtccgc cgccccccgg agtggagggg cggagctggt cgatcacgat cgcgccgaga
     2041 gaaggagtcc tggtggccac ggacaagacc taccccactg ccccggccga tacaacggat
     2101 ccggacgatc agcccatctc ggatcccacc cgacgcagct ggtcgcgcag gtccgcatag
     2161 gcgtcttcga ccgagccgtc ccgcaggcga tccagcgtcc cgtcgtcgcg gagccggtcg
     2221 gcgaggtcga tcaccgcacc gtggccacgc tcccggaggc cggcgtcgac gtgcgcccgg
     2281 gaccccgcac ccagacgggc cagcagcacc gcgaacacgc cgacctcgtc acgacggggg
     2341 ccttcgaccg gcgcgtcgga ccggcggccc agccaggcgt tgaggtggct gaacgcgccg
     2401 gcgccgtccc agagcgggtc cgggccggcg atgcgcaccc gggcgacagg tgccatgcgg
     2461 atggcggcgg cacactgcag gcacggctgg agcgtggtcg agagcaccag cccccgtggc
     2521 gcaccgaagg gcagctgcgc cagcacattg atctcggcgt gggcgagcgt cgtgccgaac
     2581 acctggcctc ggggtgcgcg atcgtcccgc acccggttgc ggccgacggc cacgatggtt
     2641 ccgtcggagt cgctcacgac cgcgcccacg ccgatgttcc cggtgacgag cgcctcccac
     2701 gcctggcgga acgcctcctg ccagaccgcg tcgagcgcgt cccacccgtc ctcccccgcg
     2761 gcgtgcacga cgacaacgta cgtcgcgtgc aacaggggca ctgccagttc cagggaggcg
     2821 gctgcacggc tcctgcgggt gccccggtac cgtcgcggcc gatgaaggcc ctccgaagct
     2881 tcaccgtccg ccccacgctg cccgccgagc tcgcgccgct cgaggcgctg gccatgaacc
     2941 tgcgctggtc gtgggacgtg cagacccgcg agctgttccg ctgggtggac cccgaccagt
     3001 gggacgccgt cgtccacgac ccggtgcggc tgctcggcag cgtcagccgg agccggctcg
     3061 acgagctcgt gcaggacaag ggcttcctgc gcttcctcga gaccgtgagc tccgacctcg
     3121 agcggtacct gtcccgcgac gcctggttcc agggccgcac cgacagcccg ctgcgctccg
     3181 tcgcctactt ctcccccgag ttcggcatct ccgaggcggt cccccagtac tccggcggcc
     3241 tcggcgtgct ggccggcgac cacctgaagg cggcgagcga cctcgggctg cccctcatcg
     3301 gcatcggcct catgtaccgc aacggctact tccgccaggc gctcaacgtg gagggctggc
     3361 aggagcagcg ctacccgaac ctcgatccct acggcatggc cctcacgctg caggaggggg
     3421 tgcgggtgag cgtcgacctc ggtgggcgcg cgctcaccgc gcaggtgtgg aaggccgagg
     3481 tcgggcgcac cgacctgtac ctgctcgaca gcgacgtcga cgacaacgac gacgacctgc
     3541 gctcggtcac cgaccgcctc tacggcggtg acagcgagca ccgcctccgc caggagatgc
     3601 tcctcggcat cggtggcgtc cgggccctgc gcgccctcgg ccgcgacacc caggtgttcc
     3661 acaccaacga gggccacgcc ggcttcctcg ggctcgagcg gatccgcgag ctcatggccg
     3721 acggcctcag cttcgagcag gccatcgagg cggtgcgcgg cggatcggtc ttcaccaccc
     3781 acacgccggt gcccgccggc atcgaccgct tcccgcgcga gctcatcgag aagtacttca
     3841 ccggcttcgc tgccgacgcc ggcatcacca tggaccagct cctggcgctc gggcacgagc
     3901 ccggcgatgc gggccagacc cggttcaaca tggcggtcat gggcttccgg ctcgcggccc
     3961 gggccaacgg cgtcgcccag ctgcacggcg aggtcagccg cgagatgttc cagcccctgt
     4021 ggcccgaggt ccccgtggag gaggtcccga tcggggccat caccaacggc gtgcacgccc
     4081 gtacgtgggt gtcctcggag atggacgatc tgctcacccg ccacgtgcac ccgcactggg
     4141 gcgaggccgg cacgcacgag tgggaccgca tccacgacgc caccgacaac gacatctggc
     4201 gggtcaagga gcagggcaag gaccgcctcg tcgccctggt ccgcgacaag atccgctcct
     4261 cggccgttgc ccgcggccag gcgccctccg acgtcgcctg ggccgacgag gtcttcgact
     4321 cccgtgcgct caccatcggc ttcgcccgcc ggttcgccac ctacaagcgg gcgacgctgc
     4381 tgctgtccca gcgcgagcgt ctcgaggcca tcctcggctc cacggagcga ccggtgcagt
     4441 tcgtgttcgc cggcaaggcc cacccggccg acgaccaggg caaggagatg atccgccaga
     4501 tcgtcgccta ctccagcgat ccctcgatcc ggcaccgctt cgtgttcctc gacgactacg
     4561 acatcgccat cgcccgcacg atgtaccacg ggtgcgacgt ttggctgaac accccgcggc
     4621 ggccccagga ggcctgcggc acgtcgggca tgaaggcagc gctgaacggc cagctcaacc
     4681 tgtcgatcct cgacgggtgg tgggacgagt gctacgacgg cgagaacggc tgggcgatct
     4741 cctcagccga gaacgtcacc gacacggcgc ggcgggacga aatcgaggcg aactcgctgt
     4801 tcgacctgct cgagcgcgag gtgatccccc tctacttcga tcgctccggc ggccggctcc
     4861 cacgccggtg ggtgcggcgc atgaagacga ccctgtccgg gctcggcccg ttcctgtcgg
     4921 cctcgcgcat ggtgaaggac tacaccaccg ccctgtacga gcctgcggct gcccacgccg
     4981 acgacctgac gggcgacgac caccgccgcg ccaaggagat cacgacctgg aaggagcgca
     5041 tcgtggccgg gtggcacgac gtgcgggtcg cgtccgtcga gagcgacggc acggtgccgg
     5101 gcatcggcga cgagcgcacc gtcgacgcgg tggtgtcgct cggcgcgctc gccggcagcg
     5161 acgtcgaggt ccagctggtg cacggcatcg tcgggcaggg cgacgagatc gaacaccccc
     5221 tgaccacgac gatgtccgag gtcgagccgc tcgacgacgg ccaggtccgc taccgcggca
     5281 gcttcgcctg cgacaccgcc ggccgctacg ggttcaccgt gcgcgtgctg ccggtccacg
     5341 aggacctggc ggcgagcgcc gagatgggcc gggtggcctg ggcctagccc gccggggctc
     5401 cctcagccct gggacgctcg gtgaggcgcc gtcccacgtc ggcggccgcc tgcggcgaca
     5461 ccacgagcac cgggaccgtc gagtgccgga cggtccgtgc cgcggtgccg ccgaggacgt
     5521 tgggacgtcc ggcgctgcga ccgtgggagc cgaggacgag cacggcgccc ggctcgaagc
     5581 ggacgtggtc gatcagcgcc cgtggcacgt cgtggccgtg gagcacatcc cagctgcgca
     5641 ccggcggctc gacccggagg gcgaggcggt gcagctcggc gctctcggcc acgtcgctgg
     5701 tgtaggccat ctcctcggtg acctcgacca gctcgatggg cacggcgaag cgccgggaga
     5761 gcgcggcggc cacgtccagg gcgccgaggg agtccgacga gccgtccacc gcgacggtga
     5821 ggcggcggag ctcgccgacc ggctcgcagg ccgggccgat gagcagcgtc ggcgccgagg
     5881 acgagcggac gaggtcggcg ctgacgctgc cgagcaccag ctcgccgagg gggccgcgcc
     5941 cgtggctgtc gagcacgacc aggtcgggat cggtgctcgc gatgtcgtgc aggagcacgt
     6001 cgaccacacc ggggccctcg aggcgacgga cctcgacgga cgcgcccggg aagcgggcct
     6061 gctcctccgc gaggaaggtg tcgacgacct cggcgggcag gccggcggtc gtgaccgcga
     6121 ccagctcgac ggtggtcgcc ggcgtggcca gggagaccgc gatctgcgcc gccgacatcg
     6181 ccgggtgggt cggatccacg gggacgagga agtgcagggg ggtcatgacc gctccttcgg
     6241 gacgacgacg acggggaccg tggcgtgctc ggtgacctgg cggctgaccg agccgaggag
     6301 catcccggcg aggccgccgt ggccccgtgc gccgacgacg agcagcgagg cgccgcgacc
     6361 tgcttcgacc agcacggttg aggccggccc ttcgatcacg agcgggcgga tcggctcggc
     6421 cagggcgccg gcgtccacct ggtcgatcac cttgcggagc accgcgtcgg cggcgtcgct
     6481 gaactcggcg gggtcgagca tgagcggcat caccgatgcg gccccgacgt aggggacgtg
     6541 gaaggcgctg accgcctcga ccacggcacc ccggaggacg gcttcggcaa ccccccagcg
     6601 gagggcggcg accgagccgt cggacccgtc cacacccacg acgatgcgtt ccatcactgc
     6661 ctcctgcggc cggtggacga tcgccatccg gctccagggg tcagcatccg tccggatcgg
     6721 acgagggcac agggtctgcg gtcccggatc cccggggccg atcggtcagc ggccggtgaa
     6781 ggcggccttg cccggcccgt gctcccggaa ggaggcgacg ccgatctggc tgtcctcggt
     6841 gcggaacgag tcggcgaaca ggcgctgctc gagccggagc ccgtcggcga ggtcgccgtc
     6901 gagaccgtcg tcgatggcct gcttggccag gccgaccacg gcgccggggc cggtggcgag
     6961 ctgcgacgcc cacgccatcg cggcgtcctc gaacccgtcg gcggggacca cccgatcgac
     7021 cagaccgatg cgcagggcct cctcggcgtc gacctggcgg ccggagaaga tgagatcctt
     7081 ggcgcgggac ggcccgacga ggcgggccag gcgctgggta ccgccgcccc cggggatgat
     7141 gccgaggagg atctcgggct ggccgaagcg agcccggtcg ctggcgatgc gcaggtcgca
     7201 ggcgagggcg agctcgcacc cgccgccgag ggcgtacccg ccgatggcgg cgacggtggc
     7261 gcgcgggatg gcggcgaccg cttcgagcgc gtcgaggaag gcaccgccga cggtctcggc
     7321 ctcctccggc cccccgaact cgctgatgtc cgccccggcg gcgaacacct tcggtccacc
     7381 ggtcacgagc accgagcggg gcgggtcgtc gatcagcgaa cgggcggcgt cgcgcagctg
     7441 gtgcagcagc gccgacgaca gcgcgttgac cttcgggttg tcgagggtga tgacggcgac
     7501 gccatcgtcg cgtcgttcca ggtgcacgag gtcagccacg cctcgaccat acgcgcgcct
     7561 ggcgacgcgt cctgctgcct cttgtgtcgc gactctggaa actggcagtc accacgtcac
     7621 acatgcgccc gcgtgacgtg gtgactgcca gttttcagtc gggagtgccg aggagctcgt
     7681 cggcggcggc ccaggggtcg gtgccgcgct gctcgacgtc gtcgaggagg tcctcgaacc
     7741 ggtcaccgtc ggacgcggcc cgggcccggg cgaggaggcg ctcggccacg atgcgggtca
     7801 gctcctcgcg ggcccgccga ccccgccgga cctggagctc gccgctggat tcgaggtggg
     7861 cgcggtgggc cagcacctgg tcccagagct cggcgatgcc cgtgccctcg gtggccacgg
     7921 ccgacaggac cggggggcgc cacggggcgc caccgtgctc ggaaccggcg ccggtgaggt
     7981 cgagcatgcc ctcgaggtcg cggcgggtct cgtcggctcc cggccggtcg gccttgttga
     8041 tcacgaagat gtcggcgatc tccatgagcc cggccttgtt ggcctggatg gcgtcgcccc
     8101 agcccgggtt gacgaccacc acggtggtgt cggcggcgcc gacgatctcg acctcgacct
     8161 ggccgacgcc cacggtctcg accagcaccc acgggtagcc ggcggcgtc
//
LOCUS       DATISU010000167         5876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_54243, whole genome shotgun sequence.
ACCESSION   DATISU010000167 DATISU010000000
VERSION     DATISU010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54243"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VLR27_06865"
     CDS             <1..183
                     /locus_tag="VLR27_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03205.1"
                     /translation="TNHLPVARWVPAEQFAAWAAEGEAMGIGHVEASPLTRSSYHARQ
                     AADHATTGPGVRTGVG"
     gene            complement(255..497)
                     /locus_tag="VLR27_06870"
     CDS             complement(255..497)
                     /locus_tag="VLR27_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03206.1"
                     /translation="MSAMKRSIRWDAMSTPAKVAVMAAASVQVSLAVSAWTDLAQRPP
                     ELVNGSKKKWAAIIAVNFLGPILYYRRGRRPAPPAG"
     gene            complement(494..955)
                     /locus_tag="VLR27_06875"
     CDS             complement(494..955)
                     /locus_tag="VLR27_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03207.1"
                     /translation="MNIDAPGITIALPAGWEAEVDHGAGEVEPGMESVRTPRVHIANF
                     PLPPDRGDFGSGAVERMLDGDALVCLLEESVGAAGSQLHGHAGVPRVREQDFSPSSMQ
                     RAIKGQSGTQVFFHDQGRAFVLYVVVGSHLSRAAVVDGINQVLAGITIHPR"
     gene            complement(952..1548)
                     /locus_tag="VLR27_06880"
     CDS             complement(952..1548)
                     /locus_tag="VLR27_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03208.1"
                     /translation="MLSSIHPLGERAKHNRFAVTATAFALGATLGGALLGTVAGLVGL
                     VVRAVLDEGVALAVLACVALVAAYFEWRGRALPSIPRQVDENWLNEYRGWVYGAGFGF
                     QLGAGVLTYITSAAVYVTIVAAVLVGNVLAAVAIGATFGLVRGLTLVPARRITDSGAL
                     VSFHRRLADTAGTVRAVSTSAIVVIGLVAALNSIQELP"
     gene            complement(1629..2444)
                     /locus_tag="VLR27_06885"
     CDS             complement(1629..2444)
                     /locus_tag="VLR27_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03209.1"
                     /translation="MTALVWLLTFVVAVLALFVVGLLRSHANIMRALHDAGINIDPDN
                     REHDHDHGSASQTPTFESSVPPTIRTVDGVPEPAGASGQRASDLVGLTPTGGTRTVAV
                     TGTDRATLIAFLTTGCATCANFWQAFAEGVELGGDTRLVVVTKGPEMESPADVAAMAA
                     PHVLTIQSTDIWDAYSVPVAPYFVLVDGRRGIVVGEGAANTWDRVRNLLDRALADAGY
                     AGNQVRRRDLLLGRARNERVDRDLRAAGLVPGDDRLYHEPAGKPDDVDDGTQA"
     gene            complement(2444..3013)
                     /locus_tag="VLR27_06890"
     CDS             complement(2444..3013)
                     /locus_tag="VLR27_06890"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HSP03210.1"
                     /translation="MSALAGPLHLVALVLVISGAQKVIDPAPPTQAMRDAGVPLLPRR
                     GTWPGRVLGVVEAATGLAVITSPWPAAAVWMGAFYLALAGFVVLLRRRDDTAGCGCFG
                     ATSAPPTRLHVVSNLLAAAVAVAAAVVGMPDIVDVMDEGIGIAVPYGVLVLTGAWLVL
                     VGPSYFASATSPPSHPAGVRPFALTSRAS"
     gene            complement(3200..4582)
                     /locus_tag="VLR27_06895"
     CDS             complement(3200..4582)
                     /locus_tag="VLR27_06895"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSP03211.1"
                     /translation="MPALESLRPTLQSALDASLKTHAVPGAVVGVLRGDERLVVAGGV
                     ANVGTGVETTPDTLFQLGSISKVYTATLVLQLVHAGLVELDEPVRGALQEFRVKDDTA
                     TLTITPRQLLAHTSGIQGDHFLDCGRNPDALWRYVSTLEDVGQLHDPDDMFSYCNAGY
                     GVLGRLVEEATGDHYARALQRRLLRPLALDHTVTQAEQAIVHRVAAGHHHDEDGTPSV
                     APWVLSRFNAPIGGIIASADDVLTFAAMHLHGGRSVEERQVVPAKAIAEMCNGEVELP
                     GSEDEWGLGWWLTRWDDQQVLAHDGDTAGQRAFLRVLPELGAAVIVLTNSTRGTAVAD
                     DVFAHVGKENLGCTPPAIPDTLPPGEGPDAERYTGIYERMHQRLAIAGGRDEGTLRMT
                     IIPDELFVKAGLRERTLTLHPVDDHRFVTLDPETLVRHLVVMVESDEDDDPDGVPYVH
                     FQRRAHARIS"
     gene            4680..5786
                     /locus_tag="VLR27_06900"
     CDS             4680..5786
                     /locus_tag="VLR27_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HSP03212.1"
                     /translation="MFADRVDRVRARMQDLDVQVLLLSVGPELPYLTGYEAMPLERLT
                     MLVLPVDGDATLLVPRLEAPRVIDRPDVFRLEAWDETDDPIARTAELVGGASTVAVGD
                     RMWSRFLVDLLPAVGVERWVRGNDVVGPIRAVKDAAEIEALLAAGAAADRVAAQLQSG
                     QIPLRGRTEAEVSADIGRRLVDEGHHKVNFAIVAAGPNASSPHHEPGSTVIEDGQAVL
                     CDFGGTMHGPGGVGYCSDMTRVVAVGSAPNDLVTAYAALEAAQRAAVDGAVVGATCES
                     VDRIARQHLVDAGWGEWFIHRTGHGIGIEEHEDPYIVEGNATPLVAGHAFSVEPGVYP
                     AGRWGIRLEDIVVATDDGPVACNQADHGLVVVDA"
BASE COUNT          794 a         2038 c         2260 g          784 t
ORIGIN      
        1 accaaccacc tccccgtggc ccgctgggtc cccgccgagc agttcgccgc ctgggccgcc
       61 gagggcgagg ccatgggcat cggccacgtc gaggcgtcgc cgctcacccg ctcgagctac
      121 cacgcccgcc aggcggccga ccacgccacc accggcccgg gcgtgcgcac cggcgtcggc
      181 tgaggctggc gattcgttct gggccgcgga gcacgcctcg gaggcgtgcc cggctgcccg
      241 gttccctggt cgtgctaccc ggcgggcggg gcgggccgac gtccgcggcg gtagtacagg
      301 atcggaccga ggaagttgac ggcgatgatc gccgcccact tcttcttcga cccgttgacg
      361 agctcgggag gtcgttgggc caggtccgtc caggccgaca cggcgagcga cacctggacg
      421 gacgcggccg ccatgaccgc gaccttcgcc ggtgtcgaca tcgcatccca gcggatcgag
      481 cgcttcatcg cgctcaccgg gggtggatcg tgatgccggc gaggacctgg ttgatgccgt
      541 cgacgaccgc cgctcgggac aggtggctgc cgacgacgac gtagaggacg aaggcccggc
      601 cctggtcgtg gaagaagacc tgggtgccgg actggccctt gatcgcccgc tgcatcgagg
      661 acggggagaa gtcctgctcc cgcacccggg ggacgcccgc atggccgtgg agctgggagc
      721 cggcggcgcc caccgactcc tcgagcaggc agaccagcgc atccccgtcg agcatgcgct
      781 cgacggcgcc gctgccgaag tcgccccggt ccggcgggag cgggaagttg gcgatgtgga
      841 cccggggggt gcgcacgctc tccatgccgg gctcgacctc gccggcgccg tggtcgacct
      901 cggcttccca cccggcgggc agggcgatcg tgatgccggg ggcgtcgatg ttcatgggag
      961 ctcctggatc gagttcagcg ccgcgaccag accgatgacg acgatcgcgg aggtggagac
     1021 ggcacggacg gtaccggcgg tgtcggccag ccggcggtgg aagctgacga gggcgccgga
     1081 gtcggtgatc cgccgggccg ggacgagggt cagcccgcgg acgaggccga aggtggcgcc
     1141 gatggcgacc gcggccagca cattgccgac caggacggcg gcgacgatcg tgacgtacac
     1201 ggccgccgat gtgatgtagg tgagcacgcc ggcgccgagc tggaagccga accccgcgcc
     1261 gtagacccag ccccggtact cgttgagcca gttctcgtcg acctggcggg ggatcgacgg
     1321 cagcgctcgc ccgcgccatt cgaagtacgc cgcgaccagg gcgacgcacg cgaggacggc
     1381 gagggcgacc ccttcgtcga ggaccgcccg gaccacgagg ccgaccaggc cggcgacggt
     1441 gccgagcagc gccccgccga gggtggcgcc gagggcgaac gccgtggcgg tcaccgcgaa
     1501 tcggttgtgc ttggcccgct ccccgagcgg gtggatgctg gacagcatcg actcacctca
     1561 cggcgaccag gtcgagcgga tggcgccgat gaccgcagcc gccatcccga tcaccaggat
     1621 cgtcggactc acgcctgggt gccgtcgtcg acgtcgtcgg gcttgccggc cggctcgtgg
     1681 tagaggcggt cgtcaccggg gacgaggccg gcagcccgca ggtcccggtc gacgcgctcg
     1741 ttccgggccc ggccgaggag caggtcccgg cggcggacct ggttgccggc gtagccggcg
     1801 tcggcgagcg ctcggtcgag caggttgcgc acccggtccc aggtgttcgc ggcaccctcg
     1861 cccaccacga tgccgcgccg accgtcgacg aggacgaagt acggggcgac cgggaccgag
     1921 taggcgtccc agatgtcggt ggactggatg gtgaggacgt gcggcgcggc catggcggcc
     1981 acgtcggcgg ggctctccat ctccggcccc ttggtgacca ccacgagccg ggtgtcgccg
     2041 ccgagttcca cgccctcggc gaaggcctgc cagaagttgg cgcacgtggc gcatccggtg
     2101 gtgaggaagg cgatgagggt ggcccggtcg gtgcccgtga ccgccacggt gcgggtgccg
     2161 cccgtcgggg tcaggccgac caggtcggag gctcgctgcc cggaggcgcc ggccggctcg
     2221 ggcacgccgt cgacggtgcg gatcgtgggc ggcacgctgg actcgaacgt cggggtctgc
     2281 gacgccgatc cgtgatcgtg gtcgtgctcc cggttgtcgg gatcgatgtt gatgccggcg
     2341 tcgtgcagcg cccgcatgat gttggcgtgg ctgcgcagga ggccgacgac gaagagggcg
     2401 aggacggcca cgacgaaggt caggagccag acgagtgcgg tcatcaggag gctctcgagg
     2461 tcagggcgaa gggccgcacg ccggcggggt gcgagggcgg cgacgtcgcc gaggcgaagt
     2521 aggacgggcc gaccagcacg agccaggcgc cggtcaggac cagcacaccg tagggcaccg
     2581 cgatcccgat gccttcgtcc atgacgtcga cgatgtcggg catgccgacg accgcggcag
     2641 ccacggccac ggcggcggcg agcaggttgg agacgacgtg cagccgggtg ggcggagccg
     2701 acgtggcccc gaagcaaccg cagccggcgg tgtcgtcacg gcggcggagc agcacgacga
     2761 agccggccag ggccaggtag aacgcgccca tccagacggc ggcggccggc cacggtgagg
     2821 tgatgacggc gagcccggtg gcggcctcca ccacgccgag cacccgaccc ggccacgtgc
     2881 cccgccgggg gagcagcggc acgccggcgt cgcgcatcgc ctgggtgggc ggcgcggggt
     2941 cgatcacctt ctgggcgccg ctgatgacga gcaccagggc gaccaggtgc agcggtccgg
     3001 ccagtgcgct caccgcgggg ggatcctgct cggcacggcg gtccaaccta gtgggtcggt
     3061 ctcggcgtac cgaccggtag gttctgcccc ggtgagcacc cgacgcctga tcatcgcggc
     3121 cctcctgtgc ggcctggcca tcctcgtggc cttctccatc cagctcctgc tggtgctgta
     3181 ggtcgcccgc gctcgcgggt caggagatgc gggcgtgagc ccggcgctgg aagtgcacgt
     3241 agggcacgcc gtcggggtcg tcgtcctcgt cggactccac catcaccacg aggtggcgga
     3301 ccagggtctc ggggtcgagg gtgacgaacc ggtggtcgtc gaccgggtgc agcgtgagcg
     3361 tgcgctcccg cagaccggcc ttcacgaaca gctcgtcggg gatgatcgtc atccgcagcg
     3421 tgccctcgtc gcggccgccg gcgatggcga ggcgctggtg catgcgctcg tagatgccgg
     3481 tgtagcgctc ggcgtcggga ccctcgcccg gggggagcgt gtcggggatc gccggtggcg
     3541 tgcacccgag gttctccttg ccgacgtggg cgaacacgtc gtcggcgacc gcggtgcccc
     3601 gggtggagtt ggtgagcacg atgacggcgg caccgagctc gggcagcacc cgcaggaacg
     3661 ctcgctggcc ggcggtgtcg ccgtcgtggg ccaggacctg ctggtcgtcc cacctcgtca
     3721 gccaccagcc cagcccccac tcgtcctcgc tgccgggcag ctcgacctcg ccattgcaca
     3781 tctcggcgat ggccttggcc ggcacgacct ggcgctcctc gacggatcgc ccgccgtgca
     3841 ggtgcatggc ggcgaaggtc agcacgtcgt cggcgctggc gatgatgccg ccgatcgggg
     3901 cgttgaagcg tgacaggacc cagggggcga cggacggcgt gccgtcctcg tcgtggtggt
     3961 ggccggcggc gacccggtgg acgatggcct gctcggcctg ggtgaccgtg tggtcgagcg
     4021 cgagggggcg caggaggcgg cgctgcaggg cccgggcgta gtggtcgccg gtggcctcct
     4081 cgacgaggcg gccgagcacg ccgtagccgg cgttgcagta ggagaacatg tcgtcggggt
     4141 cgtggagctg gcccacgtcc tcgagggtgg acacgtagcg ccacagcgcg tcggggttgc
     4201 ggccgcagtc gaggaagtgg tcgccctgga tgccgctggt gtgggccagg agctggcggg
     4261 gggtgatggt gagggtggcc gtgtcgtcct tgacccggaa ctcctgcagg gcgccccgca
     4321 ccggctcgtc cagctccacc aggccggcgt gcaccagctg gagcacgagg gtggcggtgt
     4381 acaccttcga gatcgacccg agctggaaga gcgtgtcggg cgtggtctcc acgccggtgc
     4441 cgacgttggc cacgccgccg gcgacgacga ggcgctcgtc gccccgcagc acgccgacca
     4501 ccgcaccggg cacggcgtgg gtcttcagcg acgcgtccag ggcggactgc agcgtcgggc
     4561 ggagggactc gagggccggc atgcggctga ggagggtagt ggctgggggt tctggggtcg
     4621 tccctcggga gcctccggcg atggtcggcc cggaggggcg gggctctacc ctcggggcca
     4681 tgttcgccga ccgcgtggac cgggtccggg cccggatgca ggacctcgac gtgcaggtcc
     4741 tgctgctgtc cgtcggcccc gagctgccct acctcaccgg ctacgaggcg atgccgctcg
     4801 agcgcctcac gatgctcgtg ctgcccgtcg acggcgacgc cacgctgctc gtcccccggc
     4861 tggaggcccc ccgggtcatc gaccggcccg acgtgttccg gctcgaggcc tgggacgaga
     4921 ccgacgaccc catcgcccgc acggccgagc tcgtcggcgg cgcgtccacg gtggccgtgg
     4981 gggaccggat gtggtcccgc ttcctggtcg acctcctgcc cgccgtgggc gtcgagcgct
     5041 gggtccgggg caacgacgtc gtcggcccga tccgggccgt gaaggacgcg gcggagatcg
     5101 aggccctgct ggccgccggc gccgcggccg accgtgtcgc agcccagctc cagtccggcc
     5161 agatcccgct gcgcggccgc accgaggctg aggtctccgc cgacatcggc cgccgcctcg
     5221 tcgacgaggg gcaccacaag gtcaacttcg ccatcgtggc cgccggcccg aacgcgtcct
     5281 cgccgcacca cgaaccgggc agcacggtga tcgaggacgg ccaggccgtg ctgtgcgact
     5341 tcggcggcac gatgcacggc cctggcggcg tcgggtactg ctcggacatg acccgcgtgg
     5401 tcgccgtcgg cagcgcgccg aacgacctgg tcacggcata tgccgcgctc gaagccgccc
     5461 agcgggccgc cgtcgatgga gccgtcgtcg gcgcgacctg cgagtcggtc gaccgcatcg
     5521 cccgccagca cctcgtcgac gccggttggg gtgagtggtt catccaccgc accgggcacg
     5581 gcatcgggat cgaggagcac gaggacccct acatcgtcga gggcaacgcc acgccgctcg
     5641 tggccgggca cgcgttctcc gtcgagccgg gcgtgtaccc ggccggtcgc tggggcatcc
     5701 gcctcgagga catcgtggtc gccaccgacg acggccccgt ggcgtgcaac caggccgacc
     5761 acggcctcgt cgtcgtcgac gcctgagcac cgcatgatcg agctcgacgc cgcaacgttc
     5821 tgcctgcagt gggccaccgg cggcctgctg ttcctgtggg tcaccacccg ccggcg
//
LOCUS       DATISU010000168         7724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_287337, whole genome shotgun sequence.
ACCESSION   DATISU010000168 DATISU010000000
VERSION     DATISU010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287337"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..2774
                     /locus_tag="VLR27_06905"
     CDS             192..2774
                     /locus_tag="VLR27_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSP03213.1"
                     /translation="MRVPLIGREALLRSIQARTRAAGGVLLVGDAGVGKSRLLDEVLA
                     RRTDRDWFVQRHVATAAAQTIAFGAVCTLLPQTTAVDGGQLLARVRTALVEEAAGRRI
                     LLGLDDAHLLDASSTALLCELAHRGDAVLLLSARTGEPLPPAISGLWTSGLLDRTEVP
                     PLDRSGAGELAAHLLGGDVDEALVAALVASSGGSPLLLRELVLDGRSSGAIVRRTRGW
                     ELAGSLRAGPRVQDLVRARAQHLGAPARALLETICVGEPLGLGTLQHDEATVLDGLER
                     IGLAVVEAGGAGPVIRAAHPLVSDAVVSSLSVRRRIELERDLAARLIGSGPSAHGDAL
                     RAARWLTGAGAPVPGDVLTTAATEALAGSATEEARDLADRAVAVGAGAPALLIVAETA
                     RRGGRPEESDAAARRALELATTDEERVEAQYIRALLLTHHFGDPAAAVTLLEETAAEI
                     ADPALADHLGDVAVSLAGPLGGYPTLVEETAASLESVRDGPSEFWLRMNLAFAQVMLG
                     RLDRVEEHIERALELAPETPDVGPELVDFLWAMRTGVHVQRGELASGGEAVARHMERC
                     ALDARGHATTAAIYGQLLLHAGSTDLFAVVDDVGAGRAGPDPFGATPILVGTAALGHL
                     RCGRPEEAATLLAQVPDDLSDDRALPFVGAARAALLDARGAHAEAAELLEATGRRALE
                     TTHIAFGVVAMHALVGAGAPGAATRLLAELPPLDGAMLRTMVDHATAWLDSDVAEIAA
                     AGTSFASYGALPHAATAFAQTAGFQVPGSPERQRHAARAAAWAAASAPFRPVAPVPAT
                     DLSERELDICRRAADGERSRDIGDALFLSVRTVDNHLRSAYRKLELGSRQELADVLAP
                     LPPR"
     gene            complement(2789..3727)
                     /locus_tag="VLR27_06910"
     CDS             complement(2789..3727)
                     /locus_tag="VLR27_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSP03214.1"
                     /translation="MTGETAMRIGVQLGMHGHSGSQELAAPRWEDMRRQAEAAEAAGF
                     DLLVLEDALVSGGLGTHGYWEAMTLAGAVAASTASIGIGHSMVNPPLRSPAVVAQAAA
                     TLDEISGGRYVLGIGAGNTPEDYETFGIAADHRYSRAAEAIEIVHGLVRAGAVHLDGR
                     FHAVHGSVEPRGPRSSGPPIVVGARGPRMMRLVARFADQWNAWTPDAQTVAAFRPMVE
                     ELERACEDVGRDPTTLGRTLDVAVDPHALLGEEPTWISDFLISGSAVEIADGLCAFGA
                     IGIDEVRCMVWPDRPAASRPAIIEALADVVELVHAG"
     gene            complement(3724..4227)
                     /locus_tag="VLR27_06915"
     CDS             complement(3724..4227)
                     /locus_tag="VLR27_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03215.1"
                     /translation="MMGRGQGIGRGALTMVVVAVLAGAGCSDDDTSAADAAEAATATS
                     EPTEESEGTTTTVAAASSGGTSAGSVTIGSTEYGFDPTIQCSLYPGGVVSIAGTAVDD
                     PDVEIVFDAFEPGRNQLSVTGPDFQWMTTTSDFEVEIDDPEVRGTAEVQGVDGTTEES
                     SFTFRCG"
     gene            4414..5130
                     /locus_tag="VLR27_06920"
     CDS             4414..5130
                     /locus_tag="VLR27_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03216.1"
                     /translation="MVDGGPAGVWRRAGTAGATLALLALASGAAWVSARPSTLEAGDV
                     TPAAAPASSAEAAPAPSTTSATTTTVETDPPSPGDLDPRWFDGTRPERIRLVYDLQRN
                     DGSRTEVVYSRDGSRWSVRDDRYWFVHVDGTSVRCMLRVACQESTKPEPPWFGLGDLL
                     YLPTPTSEGERRRIAGIPARCWTGDVIAWTVCHAEEDGLLLLSDPGPDAMKPSAFESA
                     RTELVRVEEPRESDFASPSA"
     gene            5150..5527
                     /locus_tag="VLR27_06925"
     CDS             5150..5527
                     /locus_tag="VLR27_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03217.1"
                     /translation="MFDLLSVILESVPGAPGPPMPPAYSVVGPVTGAPSCPLDAPPTD
                     PGAEPQPKDVPIFGAKGKDYLTVRALDHLGDTYKQDRSIVNAAKLLVTLPLLAPLVVL
                     EDTFRNPVPGVHGYRRKSGCPHG"
     gene            5520..6695
                     /locus_tag="VLR27_06930"
     CDS             5520..6695
                     /locus_tag="VLR27_06930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HSP03218.1"
                     /translation="MADVAVTAVGPPTLWDDDALAAIERGRLLAEPPAPAPLLDRYIS
                     WVTRAPRHLRSTVSMSISLLLVVASIVTRPDVDLAHVRTVIWHQVLGIGLLLLATALT
                     RAVALRRVVSFWLFGYFLAGPAVDRIGPLLDERVEAELFQAGVVPVLEEGIKLVPVLV
                     FAVVRSRRGWTPAATDLMVLGAAVGGGFALYEDLLWDRVASSGLGGLGTLLPTMLRDP
                     FLASGHVVWTACGALGIGVMVLHGRRVWPFVLGPVLVVLPTLDHAVINMTSDRRDGLR
                     GVLLDGRLVPYLLLGTLAAAVILEAGLRRRAAACDPVLPTPTFSQVVFAPPAPLFGGG
                     RSLRHYVRRRNGFVYTSLAGSGSRAPSPEEEGFALGWLEAMSRRPSTDQTRSETDAR"
     gene            6709..7083
                     /locus_tag="VLR27_06935"
     CDS             6709..7083
                     /locus_tag="VLR27_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03219.1"
                     /translation="MLDASASAAGALALALVLAGCGGDSAPEADAAAATDRSGEVRSP
                     SPDIEEADGTGRSLDQLATAASGALSAERYEVTDGDGIRFIMGPGSSVDSTTGLCLGA
                     ATVLPDGTDITLVFPDGELRCD"
     gene            7171..7620
                     /locus_tag="VLR27_06940"
     CDS             7171..7620
                     /locus_tag="VLR27_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03220.1"
                     /translation="MSATLTPLFDGFAADPLFPVDPELVVADPPSLQDGLCAAALLWC
                     THSTWPTVRAIASCAGISPSSVLWPYGSIEQMRLALIRAERAALASLVEAHGDDLPLA
                     VDERTEQLCGYDLRLSKLPLLVALAAGVHDATELATLAGAVRSLPAA"
BASE COUNT          986 a         2975 c         2671 g         1092 t
ORIGIN      
        1 tcgaagggct ggacgttcag ctccgaccag ctccggaact ggtcctccac gggcatgcct
       61 ggctgatcca acgggttcat gagggtctcc gatcgacgcg cgggaccact ccctgtccct
      121 gagacggtcg tcacagacct cggaggctcg gccacccgtc cgcacccgcg atgccggtac
      181 cgtccacgac gatgcgagtg ccgctgatcg gccgagaggc gctgctccgc agcatccagg
      241 cacggacccg agccgccggg ggcgtcctgc tcgtcggcga cgcaggggtg ggcaagagcc
      301 gcctgctcga cgaggtcctc gcccggcgca cggaccgtga ctggttcgtc cagcgacacg
      361 tcgccaccgc agcagcgcag acgatcgcct tcggtgcggt gtgcaccctg ctcccccaga
      421 ccacggcggt ggacggcggg cagctgctcg cccgggtgcg gaccgcgctc gtcgaggagg
      481 ccgccgggcg ccggatcctc ctcggcctcg acgacgccca cctgctggat gcctcctcga
      541 ccgcactcct ctgcgagctc gcgcaccggg gcgatgcggt cctgctgctg tccgctcgca
      601 ccggagaacc gctgccgccg gcgatcagcg ggctgtggac gtcggggctg ctcgaccgga
      661 ccgaggtccc accgctcgac cgcagtggcg ccggcgagct ggccgcgcac ctgctcggcg
      721 gcgacgtcga cgaggcgctg gtcgccgcgc tcgtggcgtc cagcggtggg agcccgctgc
      781 tgctgcgaga gctcgtcctg gacggacgca gctccggcgc gatcgtccgc cggacacgcg
      841 ggtgggagct ggcgggctcg ttgcgggcgg gaccacgcgt ccaggacctc gtgcgggcca
      901 gggcccagca cctgggtgca ccagcccgag ccctcctcga gacgatctgc gtcggtgagc
      961 cactcggcct cggcacgctc cagcacgacg aggcgaccgt gctcgacggc ctcgagcgca
     1021 tcggcttggc cgtggtggag gctggcggcg ccggtcccgt catccgcgcc gcgcatccgc
     1081 tcgtgtccga cgccgtcgtg tcctcgctgt cggtccggcg gcggatcgag ctcgagcgcg
     1141 acctggcggc gcggctgatc ggatccgggc cgtccgccca cggcgacgcc ctccgtgccg
     1201 cccgctggct caccggcgcc ggcgcaccgg tccctggcga cgtgctcacc acggcggcca
     1261 ccgaagccct cgccggttcg gccaccgagg aggcccgtga cctcgctgac cgggccgtcg
     1321 ccgtcggcgc gggcgcaccc gccctgctca tcgtggccga gaccgcgcga cgagggggcc
     1381 gacccgagga gtccgacgcc gccgcacggc gagcactcga gctcgccacc acggacgagg
     1441 agcgggtcga ggcgcagtac atccgagcgc tcctgctcac ccaccacttc ggggaccccg
     1501 ccgccgccgt cacgctgctg gaggagaccg ccgcggagat cgccgacccg gcgttggccg
     1561 atcacctcgg ggacgtcgcc gtcagcctgg ccggtccact cgggggctac ccgacgctcg
     1621 tggaggagac cgcggcctcg ctcgagtcgg tccgggatgg tccgtcggag ttctggctgc
     1681 ggatgaacct cgccttcgcc caggtgatgc tcggacggct cgatcgcgtc gaggagcaca
     1741 tcgagcgcgc gctggagctc gcgccggaga ccccggacgt cggaccggag ctggtcgact
     1801 tcctctgggc catgcggacc ggcgtgcacg tgcagcgggg cgagctcgcg agcggaggtg
     1861 aggccgtcgc ccggcacatg gagcgctgtg ccctcgacgc tcgaggccat gccacgacgg
     1921 cagcgatcta cggccagctc ctgctccatg ccggctcgac cgacctcttc gccgtcgtgg
     1981 acgacgtcgg cgcgggccgg gccgggccgg acccgttcgg tgcgacgccg atcctggtcg
     2041 gcaccgccgc cctcggtcac ctgcgctgcg gtcgacccga ggaggcggcc acgctgctcg
     2101 cccaggtgcc cgacgacctc agcgacgatc gggcgctgcc gttcgtcggt gcagcacgag
     2161 cggccttgct cgacgcaagg ggcgcgcacg ccgaggccgc ggagctcctc gaggcgaccg
     2221 gcagacgcgc cctggagacc acccacatcg cgttcggggt cgtcgccatg cacgcgctgg
     2281 tcggcgccgg cgcgccgggc gccgccaccc gactgctggc cgagctgccg ccgctcgacg
     2341 gagcgatgct gcggacgatg gtcgatcacg ccacggcgtg gctcgacagc gacgtggccg
     2401 agatcgctgc cgctggcacc tccttcgcgt cgtacggggc gctcccccac gccgcgaccg
     2461 cgttcgccca gaccgcggga ttccaggtac cgggttcacc cgagcgccag cggcacgcgg
     2521 cgcgggctgc ggcctgggcg gcggcatcgg ctccgttccg tcccgtcgcc cccgtcccgg
     2581 cgacggacct gtcggagcgg gagctcgaca tctgccggcg ggccgccgac ggggagcgga
     2641 gccgcgacat cggcgatgcc ctcttcctgt ccgtgcgcac cgtcgacaac cacctccgca
     2701 gcgcctaccg aaagctcgag ctcggcagcc gccaggagct cgccgacgtg ctggcaccgc
     2761 tcccgcctcg ctgaaccgcc tcggcggttc agccggcgtg cacgagctcc accacgtccg
     2821 ccagcgcctc gatgatcgcc ggccggctcg ccgccggccg gtcgggccac accatgcagc
     2881 ggacctcgtc gatcccgatc gcaccgaagg cgcacagacc gtcggcgatc tcgaccgctg
     2941 atcccgagat gaggaagtcc gagatccacg tgggttcctc gcccaggagc gcgtgggggt
     3001 cgacggcgac gtcgagcgtc cgcccgagcg tggtcgggtc acgtcccacg tcctcacagg
     3061 cccgctcgag ctcctcgacc atcggtcgga aggcggcgac cgtctgggca tccggggtcc
     3121 aggcgttcca ctggtcagcg aaccgggcca ccaggcgcat catcctcgga ccgcgagcgc
     3181 cgaccacgat cggcgggccg gacgagcgcg gtccccgcgg ctcgaccgag ccgtgcacgg
     3241 cgtggaagcg cccgtcgagg tgcacggcgc cggcacggac cagcccgtgg acgatctcga
     3301 tcgcctcggc ggcccgggag tagcggtggt ctgccgcgat gccgaaggtc tcgtagtcct
     3361 ccggcgtgtt cccggcgccg atccccagca cgtagcgacc cccggagatc tcatcgagcg
     3421 tcgccgcagc ctgcgccacc accgccgggg agcgcagcgg cgggttcacc atcgagtgcc
     3481 cgatgccgat cgacgccgtc gaggccgcca ccgcaccggc cagcgtcatg gcctcccagt
     3541 agccgtgggt gccgagcccg ccgctgacca gcgcgtcctc gaggacgagc agatcgaacc
     3601 cggccgcctc cgcagcctcc gcctggcggc gcatgtcctc ccaccggggc gcggccagct
     3661 cctggctccc ggagtggccg tgcatcccga gctgcacccc gatgcgcatc gccgtctccc
     3721 ccgtcacccg caacggaagg tgaaggagct ctcctcggtg gtgccatcca cgccctggac
     3781 ctcggccgtg ccccgcacct ccggatcgtc gatctccacc tcgaagtcgg acgtggtcgt
     3841 catccactgg aagtccggtc cggtcacgct gagctggttc cgtccgggtt cgaacgcgtc
     3901 gaagacgatc tccacgtcgg ggtcgtcgac cgccgtccct gcgatgctca ccacccctcc
     3961 gggatagagc gagcactgga tggtcgggtc gaagccgtac tccgtcgacc cgatggtgac
     4021 gctgccggcc gaggtgcctc cgctcgaggc tgcggcgacg gtggtcgtcg tgccctcgga
     4081 ctcctcggtc ggttcgctcg tcgccgttgc ggcctcggcg gcatcggccg ccgaggtgtc
     4141 gtcgtccgag cagcccgccc ccgccagcac ggcgaccacc accatggtca gcgcaccccg
     4201 cccgatcccc tgtcctcgtc ccatcacgtc gttccacctc tcctctggtc ggtccacgcg
     4261 atcgtcgggc acgcagcgcc gggcgaccac ggcctgcgct actcaacgcc gagccgccgt
     4321 tgctcaacgc gccggggaga ttgagcagtc gcccggcgcc gatgagtacc tcctcccgtt
     4381 gcccaccggg aggtgcgcgc cgacgctggg ggcgtggtgg acggtggacc cgcaggggtc
     4441 tggagacggg ccgggacggc gggggcgacg ctggcgctgc tcgcgctggc gtccggggcg
     4501 gcatgggtct cggcccggcc gtccaccctc gaggcgggtg acgtcacgcc cgccgcggca
     4561 ccggcatcga gcgccgaggc ggcaccggcg ccgagcacga cctccgccac caccacgacc
     4621 gtcgagacgg atcccccgag ccccggcgac ctcgatcccc gatggttcga tggcacgaga
     4681 cccgaacgga tccgcctggt ctacgacctc cagcgcaacg acggcagccg caccgaggtg
     4741 gtctactccc gggacgggag ccgctggagc gtgcgagacg accggtactg gttcgtgcac
     4801 gtcgacggca ccagcgtgcg ctgcatgctc cgcgtcgcct gccaggagtc gaccaagccg
     4861 gaaccgccgt ggttcgggct cggggacctc ctctacctgc ccacgccgac ctccgagggc
     4921 gaacgacgcc ggatcgccgg gatcccggct cgctgctgga cgggcgacgt catcgcttgg
     4981 acggtgtgcc atgccgagga ggacgggctc ctcctgctct ccgatccagg gcccgatgcg
     5041 atgaagccca gtgcgttcga gagcgcgcgc acggagctcg ttcgcgtgga ggagccacgc
     5101 gaatcggact tcgcgagccc atccgcgtga ccccacgaag gagcatcccg tgttcgacct
     5161 cctgagcgtg atcctcgagt ccgtcccggg tgcgccgggc ccgccgatgc cgccggcgta
     5221 cagcgtcgtc ggaccggtca ccggggcgcc gtcgtgtccc ctggatgcac cgccgaccga
     5281 tcccggggcg gagccccagc cgaaggacgt gcccatcttc ggcgcgaagg gcaaggacta
     5341 cctgacggtc cgcgccctcg atcacctggg cgacacgtac aagcaggacc gatccatcgt
     5401 caacgcggcg aagttgctgg tgacccttcc cctgctggcg ccgctggtcg tgctggagga
     5461 caccttccgc aaccccgtgc ccggcgtgca cggctaccga cggaagtccg ggtgccccca
     5521 tggctgacgt ggcggtgacg gcagtcggac caccgaccct ctgggacgac gatgcgctcg
     5581 cggccatcga gcgaggacgg ctcctcgccg agcctccggc cccggcgccg ctgctcgatc
     5641 gctacatctc ctgggtgacg cgcgccccgc gccacctgcg atcgacggtg tcgatgtcga
     5701 tctcgctgct gctggtggtc gcatccatcg tcacccgtcc cgacgtcgac ctggcccacg
     5761 tccggaccgt gatctggcac caggtcctcg gcatcggtct cctgctgctc gccaccgccc
     5821 tcacccgggc cgtggcgctg cgacgggtgg tgagcttctg gctgttcggc tacttcctcg
     5881 ccggaccggc ggtggatcgc atcggtccgc tcctcgacga gcgcgtcgag gccgagctgt
     5941 tccaggccgg cgtcgtcccc gtgctggagg agggcatcaa gctggtgccg gtgctcgtgt
     6001 tcgccgtcgt ccggtcccgg cgggggtgga cgccggccgc gaccgacctc atggtgctcg
     6061 gcgccgcggt gggcgggggc ttcgccctgt acgaggacct gctctgggac cgggtcgcct
     6121 catcgggtct cggcgggctc ggcacgctgc tgcccacgat gctgcgcgac ccgttcctgg
     6181 cctcgggcca cgtcgtgtgg accgcgtgcg gtgcgctcgg catcggggtg atggtgctgc
     6241 acggtcgacg ggtctggccc ttcgtgctcg gtccggtcct ggtggtgctg cccacgctcg
     6301 accacgcggt catcaacatg acgagcgacc gccgtgacgg cctgcgcggc gtcctgctcg
     6361 atggccgact ggtgccgtac ctgctgctcg gcaccctcgc cgctgcggtc atcctggagg
     6421 cggggctgcg acgacgtgcc gccgcctgcg atccggtgct gcccacgccg acgttctccc
     6481 aggtggtctt cgccccgccg gcgccgctgt tcggcggcgg gcgcagcctg cggcactacg
     6541 tccgccggcg caacggcttc gtgtacacga gcctcgccgg gtccggatct cgcgccccct
     6601 cccccgagga ggagggcttc gccctcggct ggctcgaggc catgtcccgc agaccgtcca
     6661 ccgaccagac caggagcgag accgatgcca ggtgatcaca ggaaccggat gctcgacgcg
     6721 agcgccagcg cagcaggggc gctggcgctc gcgctcgtcc tcgccggatg cggcggcgac
     6781 tccgccccgg aggccgacgc cgcggcggcc accgaccggt ccggtgaggt gcgatccccg
     6841 agccccgaca tcgaggaggc cgacggcacc gggcgctcgc tcgaccagct ggccaccgca
     6901 gcgagcggcg cgctgtccgc cgagcgctac gaggtcaccg acggagacgg gatccgcttc
     6961 atcatgggtc cgggatcgtc ggtcgactcc acgaccgggt tgtgcctcgg cgcagcgacg
     7021 gtcctcccgg acggcaccga catcaccctc gtcttccccg acggagagct ccgatgtgac
     7081 tgagcgaccg agagcgacag gcgttcctgt cagcggccgt ccgtaacgtc gacaccactt
     7141 ccccctccgg ttcgctccga cggaggaccc atgtccgcaa cgctcacccc cctgttcgac
     7201 ggcttcgcag ccgatcccct gttcccggtc gaccccgagc tggtcgtcgc cgatccaccc
     7261 tcgctgcagg acggcctgtg cgccgccgcc ctgctgtggt gcacccactc cacctggccc
     7321 accgtccggg ccatcgcgtc ctgcgccggc atctcgccct cgtccgtgct gtggccctac
     7381 ggctccatcg agcagatgcg cctcgccctc atccgcgccg agcgcgccgc gctcgcatcc
     7441 ttggtcgagg cccacggcga cgacctcccg ctcgccgtcg acgaacgcac cgagcagctc
     7501 tgcgggtacg acctgcgcct cagcaagctg ccgctgctgg tcgcgctggc cgccggcgtg
     7561 cacgacgcga ccgagctggc gaccctcgcc ggcgccgtcc gctccctacc ggccgcgtag
     7621 cggtccatgg cacagcactg gcatgctcat gccatgccgc gcgagcgagt cagcaccacc
     7681 gtcgacggcg acctcctcgc cgaagctcga gccctgcgcg ccgg
//
LOCUS       DATISU010000169         3317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_799163, whole genome shotgun sequence.
ACCESSION   DATISU010000169 DATISU010000000
VERSION     DATISU010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799163"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VLR27_06945"
     CDS             complement(<1..234)
                     /locus_tag="VLR27_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03221.1"
                     /translation="MSEIWPGRAYPLGATFDGAGTNFSLFSEIAERVELCLFSRDGRE
                     TRHELVEVDAHCWHAYLPGVGPGQQYGFRVHGPW"
     gene            complement(297..884)
                     /locus_tag="VLR27_06950"
     CDS             complement(297..884)
                     /locus_tag="VLR27_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03222.1"
                     /translation="MHRSKIFAPLVAVALLFAACGDDDDTSTATDDTVADETTETATE
                     EETEDDGGSDVDEEAMAEGLGECGFLTGFATAFEDFDPTAMYGGEEATDFGQIFAPLA
                     QATQDVAEAAPEEIRDAFRTMADGFSEVAAELEGVVIDFADPESMDPEAMAKLESLGE
                     AFGDEYEAAAEAVDAWVSENCADLADAFDLDAFGS"
     gene            complement(936..1988)
                     /gene="gcvT"
                     /locus_tag="VLR27_06955"
     CDS             complement(936..1988)
                     /gene="gcvT"
                     /locus_tag="VLR27_06955"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HSP03223.1"
                     /translation="MQRSPLDAEHRSLGAKLVPFGGWEMPLAYGEGTVSEHRACRTGA
                     VAFDVSHLGTVRVTGPDAFERLQSTLTNDLHRIEPGRAQYTHLLDADDASVLDDLIIW
                     WREDEVFDVMPNASNTDGVRDAIGGEDVTTTRAVIAVQGPDARARLATVAPAAAEVPR
                     FAVRSFDWDGVSCTVAGTGYTGEDGVECAVPAEAAPAFWQALLAAGVEPAGLGARDTL
                     RLEAGLPLHGNELGPGITPLQAGLGWVVRWDTEFRGKAALQAERDAGVTRRLRGLVCE
                     GRRPPRTGNDVHVGGVAAGTVTSGNFSPMLETGIALAFLPPEVEDGAGVEIDVRGSVV
                     PAVVTKPPFWPADRAG"
     gene            complement(2023..2493)
                     /locus_tag="VLR27_06960"
     CDS             complement(2023..2493)
                     /locus_tag="VLR27_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HSP03224.1"
                     /translation="MAGTIEGGFSGKRAAEIVGITYRQLDYWARTDLIRPSLADAGGS
                     GTRRRYSYKDLLELKVIKRLLDAGIKLESIREAFEYLRERLGEDVASAHLVIDGTSAV
                     LVRGNELIDVVNRYQGQGVLNLLPMDGVIEELDTAVQMFGDGATPDGMGTAVPG"
     gene            complement(2617..3114)
                     /locus_tag="VLR27_06965"
     CDS             complement(2617..3114)
                     /locus_tag="VLR27_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HSP03225.1"
                     /translation="MTSEMELVGVRVDMPSNTPVMLLRELEGDRRLLAIMIGGPEAQA
                     IAFALDGVETRRPLTHDLIALLLDELDAHLDRIVISALRDDVYYADLHLRVGEQDHVV
                     SARPSDALAIAVRVGTPVFADEAVLAEAGYLDVPDDEDLEDDDEVVEEFREFIDQVNP
                     EDFAS"
BASE COUNT          450 a         1174 c         1221 g          472 t
ORIGIN      
        1 ccacgggccg tggacccgga agccgtactg ctgacccggg ccgaccccgg gcaggtaggc
       61 gtgccagcag tgcgcgtcga cctcgaccag ctcgtggcgg gtctcacggc cgtcgcggct
      121 gaacaggcac agctcgacgc gctcggcgat ctcgctgaac aggctgaagt tggtgccggc
      181 cccgtcgaag gtggcaccga gggggtatgc ccgaccgggc cagatctcgg acacggcggg
      241 cacgctaccg gtgcccccgg cggcgcccgg caccgccggg agccgccggc gcgggttcag
      301 gacccgaagg cgtccaggtc gaaggcgtcg gccaggtcgg cgcagttctc gctcacccag
      361 gcgtcgaccg cttcggccgc cgcctcgtac tcgtcgccga acgcctcgcc gaggctctcg
      421 agcttggcca tcgcctccgg gtccatgctc tcgggatcgg cgaagtcgat cacgacgccc
      481 tcgagctccg ccgcgacctc gctgaagccg tcggccatcg tgcggaacgc atcccggatc
      541 tcctccggcg cggcctcggc cacgtcctgg gtcgcttggg ccagtggggc gaagatctgg
      601 ccgaagtcgg tggcctcctc gccgccgtac atcgcagtcg gatcgaagtc ctcgaacgcg
      661 gtggcgaagc cggtcaggaa gccgcactcc cccaagccct cggccatcgc ctcctcgtcg
      721 acgtcggacc cgccgtcgtc ctcggtctcc tcctcggtgg ccgtctccgt ggtctcgtcg
      781 gcgaccgtgt cgtcggtggc ggtgctggtg tcgtcgtcat cgccgcacgc ggcgaagagc
      841 agggcgacgg cgacgagcgg ggcgaagatc ttgctgcggt gcattggtca ttccctcggg
      901 aggttctgtc ggatctgcga cactaccgcg cggcctcagc cggctcggtc ggcgggccag
      961 aacggcggct tggtcaccac ggcggggacc acggagccgc gcacgtcgat ctcgacgccg
     1021 gcgccgtcct cgacctccgg tgggaggaag gcgagcgcga tgccggtctc gagcatgggt
     1081 gagaagttcc ccgacgtgac cgtgcccgct gcgacaccgc ccacgtgcac gtcgttgccg
     1141 gtgcggggtg ggcggcgccc ctcacagacg aggccgcgca ggcggcgggt cacgccggcg
     1201 tcacgctcgg cctggagcgc ggccttgccc cggaactcgg tgtcccagcg cacgacccag
     1261 ccgagcccgg cctgcaacgg cgtgatgccg gggccgagct cgttgccgtg caggggcaga
     1321 ccggcctcga ggcgcagcgt gtcccgggcg ccgagcccgg ccggctcgac accggccgcc
     1381 agcagcgcct gccagaacgc aggagcggcc tcggccggca cggcgcactc gacgccgtcc
     1441 tcccccgtgt agccggtgcc ggcgacggtg caggagacgc cgtcccagtc gaagctgcgc
     1501 acggcgaagc gcggcacctc ggcggcggcg ggcgccaccg tggcgagccg ggcgcgggca
     1561 tccgggccct ggacggcgat gaccgcccgc gtggtggtga cgtcctcccc gccgatggcg
     1621 tcccgcacgc cgtcggtgtt ggaggcgttg ggcatgacat cgaagacctc gtcctcgcgc
     1681 caccagatga tgaggtcgtc gagcaccgac gcgtcgtcag cgtccaggag gtgggtgtac
     1741 tgggcccgac ccggctcgat ccggtgcagg tcgttggtga gcgtggactg cagccgctcg
     1801 aacgcgtcgg gacccgtcac ccggaccgtg ccgaggtggg acacgtcgaa ggccacggca
     1861 ccggtccggc aggcccggtg ctcggagacg gtgccctccc cgtaggcgag gggcatctcc
     1921 cagcccccga agggaacgag cttggcgccg aggctgcggt gctcggcatc gagaggcgat
     1981 cgctgcatgc cccgagcctg gcaggtacgt ggcgacggcg gttcagcccg ggacggcggt
     2041 tcccatcccg tcgggcgtgg cgccgtcgcc gaacatctgc acggcggtgt cgagctcctc
     2101 gatgacgccg tccatgggca ggaggttgag cacgccctgg ccctggtagc ggttcacgac
     2161 gtcgatgagc tcgttgcccc gcaccagcac ggcgctggtg ccgtcgatga ccaggtgggc
     2221 ggaggcgacg tcctcgccga ggcgctcgcg caggtactcg aaggcctcgc ggatggactc
     2281 cagcttgatg ccggcatcga ggaggcgctt gatgaccttg agctcgagca ggtccttgta
     2341 gctgtagcga cggcgggtgc cggacccgcc ggcgtcggcc agggagggcc ggatcaggtc
     2401 ggtccgggcc cagtagtcga gctggcggta ggtgatgccg acgatctccg ccgcccgctt
     2461 ccccgagaag ccaccttcga tcgtgcctgc catcaccgct cctccgtcca gcgcagccag
     2521 cgggtccgac ggaccacacc agcgaaatta cgcgcgtgtc gttgcggaat ggtacgaccg
     2581 gccgcgcgct gcgtcaacgg gcgaggcccg ggctggtcag gaggcgaagt cctcggggtt
     2641 cacctggtcg atgaactcgc ggaactcctc gaccacctcg tcgtcgtcct cgaggtcctc
     2701 gtcgtcgggc acgtcgaggt agccggcctc ggccaggacg gcctcgtcgg cgaacaccgg
     2761 ggtgccgacg cgcacggcga tggccagggc gtcgctcggg cgggccgaga cgacgtggtc
     2821 ctgctcgccg acccgcaggt ggaggtcggc gtagtacacg tcgtcgcgca gcgccgagat
     2881 cacgatgcgg tcgaggtggg cgtcgagctc gtcgagcagc aaggcgatga ggtcgtgggt
     2941 gaggggccgg cgggtctcca cgccgtcgag cgcgaaggcg atggcctggg cctctggtcc
     3001 accgatcatg atcgccagca accggcgatc gccctcgagc tcccggagca gcatcaccgg
     3061 ggtgttcgag ggcatgtcca cgcggacgcc gacgagctcc atctccgaag tcatcacggg
     3121 ccaccctatc gctggtcccg cggcccccgt tgcgcccttc gctacggcag cgtgtcccgc
     3181 agcgcccggc gcaggagggc ggcgtggagc gactcccccg cgtcggcgag ctcggtgacg
     3241 gtgtcgaccg cacgggcccg ggcctcgggg ttgcgctgct tcaggagggg cacgatgagc
     3301 tgctccagca ccccggc
//
LOCUS       DATISU010000170         1577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_427259, whole genome shotgun sequence.
ACCESSION   DATISU010000170 DATISU010000000
VERSION     DATISU010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427259"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VLR27_06970"
     CDS             complement(<1..269)
                     /locus_tag="VLR27_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HSP03226.1"
                     /translation="MHTQSGYEKKVKTNLEARTASMNMEERIYEVAIPLEDVVEFKNG
                     KRVVVQKKMFPGYLLVRCRLDDDSWYVIRNTPGVTGFVGAGAKPSP"
     gene            complement(533..841)
                     /gene="secE"
                     /locus_tag="VLR27_06975"
     CDS             complement(533..841)
                     /gene="secE"
                     /locus_tag="VLR27_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HSP03227.1"
                     /translation="MAMNREQRRLLKKQGELNEQGEPVRQPRGQQQQRGPAPKSERVG
                     PRQFVGEVRGELRKVAWPSRTEVANYSVVVFLGIVVLTAMIAGLDYVLGEAVLELFAR
                     "
     gene            complement(903..975)
                     /locus_tag="VLR27_06980"
     tRNA            complement(903..975)
                     /locus_tag="VLR27_06980"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(940..942),aa:Trp,seq:cca)
     gene            complement(1028..1396)
                     /locus_tag="VLR27_06985"
     CDS             complement(1028..1396)
                     /locus_tag="VLR27_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03228.1"
                     /translation="MSAVRCADLADDLGGVADGSHQLSRPARRHVEGCLRCQADLVQY
                     RRLLRTLRTLRTDVLEPAPGLLADIFASLEHAGERHAIRGVLHGRRAAYVGGLAAATA
                     AGAAGALVLAARSRRTELAS"
BASE COUNT          200 a          527 c          608 g          242 t
ORIGIN      
        1 ggggagggct tggcgcccgc acccacgaac ccggtgacgc cgggggtgtt gcggatgacg
       61 taccaggagt cgtcgtcgag gcggcaccgc acgagcaggt agccggggaa catcttcttc
      121 tggacgacga cgcgcttgcc gttcttgaac tcgacgacgt cctcgagggg gatggccacc
      181 tcgtagatcc gctcctccat gttcatggat gcggtgcggg cctcgaggtt ggtcttgacc
      241 ttcttctcgt agcccgactg ggtgtgcacg acgtaccact tgccgggacg gtcgtagggg
      301 ctgacgacct cctcggcctc ctcggggacg ggctcgacga ggtcctcggc gtcgacgacc
      361 tggacgtcgt cggactcgga ctcggcctcg ggggcgtccg cctcgggggc ctcggcctca
      421 ggcgtctccg cctcgggcgc ggcggcctcg ggggcgtcgg cctggtccag gtcggtcgtg
      481 gcgtcggtgt cgacgtcgga cacgggggtc tcctcttcgg ggggcaggtc gctcatcggg
      541 cgaacagctc gaggacggcc tcgccgagca cgtagtcgag accggcgatc atggcggtga
      601 gcaccacgat gccgaggaac acgacgacgg agtagttggc cacctcggtg cgagacggcc
      661 aggcgacctt gcggagctcg ccgcggacct cgccgacgaa ctgccgggga ccgacgcgct
      721 cgctcttggg cgcaggccca cgctgctgct gctggccgcg cggctggcgg acgggctcgc
      781 cctgctcgtt cagctcgccc tgcttcttca gcaggcggcg ctgctctcgg ttcatggcca
      841 tgcgggtttg gacctcggga ttgcggggga tcggtgcgcg gcggcgcacc cacgggttcg
      901 agcagggcag gagggaatcg aacccccaac cgccggtttt ggagaccggt gctctgccag
      961 ttgagctact gccctcggac gcgagggttc acgctagcac cggccctggg caggacccca
     1021 tcctggatca gctggcgagt tcggtgcggc ggctgcgggc ggccagcacc agggccccgg
     1081 cggcccctgc tgcggtggcg gcggcgagac cgcccacgta cgcggcccgg cggccgtgga
     1141 ggacgccgcg gatggcgtgg cgctcccccg cgtgctcgag ggaggcgaag atgtcggcga
     1201 gcaggccggg ggcgggctcg aggacatcgg tgcgcaacgt gcgcagggtg cgcagcaggc
     1261 gccggtactg gaccaggtcg gcctggcagc gcaggcaccc ctccacgtgg cggcgggccg
     1321 ggcggctgag ctggtggctg ccatcggcga cgcccccgag gtcgtcggcg aggtccgcgc
     1381 accggaccgc gctcacggac ggggctccca gcgctcagct ggcacggatc tcatctccga
     1441 ggggctggtc ctcccgacgg gggaacaggt gctccttgag ctggcgacga gcccggtgca
     1501 ggcgcacctt ggccgcggtc tcgctgatgc cgagctcggc ggcgatgtcc tcatgcggca
     1561 gctcgtagac gtcgcgc
//
LOCUS       DATISU010000171         7676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_846426, whole genome shotgun sequence.
ACCESSION   DATISU010000171 DATISU010000000
VERSION     DATISU010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846426"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..673
                     /locus_tag="VLR27_06990"
     CDS             203..673
                     /locus_tag="VLR27_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HSP03229.1"
                     /translation="MAVAVVIGVLLLRDDDSATTQVAVGSDTAAEVDDTDVDESGDED
                     PDATTTTTTEAPRPPAEVKVLVANGSGVNGAAGGTTDALEALGYVTGTPANAERVPAT
                     VVYFTTGFQAEAEALAAAIGAPEDGVTPMPAVAPVDDLQLANVLVHLGPDLAAG"
     gene            complement(683..1501)
                     /locus_tag="VLR27_06995"
     CDS             complement(683..1501)
                     /locus_tag="VLR27_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03230.1"
                     /translation="MVPSRRVRRAVAASTLVLALAACEEHTVAVRFEPEVGDVHRFRA
                     DVETEVVRTVDGVTTDEDDAATLDATQTVQSVTDDEVLVRVALQRDDAAPRTVDVRLD
                     RASRLTAIDLVEGVPAEALGLDVAMDLPADIASPPTGRLEPGDRWEILREVSLDGLSR
                     PVLVQGRGRIDSLGVEDGRDVAVAVVELDVPVRTIIDSDDGRVRLVGTQRTTSETAYD
                     LGDGAMNRDRTVTVGTVEVIAEPPAGVDAEPIRGDIEYRIVTHSRRLSGPVTPA"
     gene            1541..2329
                     /gene="otsB"
                     /locus_tag="VLR27_07000"
     CDS             1541..2329
                     /gene="otsB"
                     /locus_tag="VLR27_07000"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HSP03231.1"
                     /translation="MPDALDVPLTGRRAEDQLRAVVGREPARTALFIDFDGTLAPIVP
                     DPASATAIDGAVELLERLGRRFRRVAVVSGRPRAFLVERVGAGVDLSGVYGLETRIDG
                     VEADHDEAHRWRSVIAEVAASGAGLPSDVLVESKGLSLTVHYREAPDAEAEVLAWADE
                     VARTAGLEVRAAKRSVELHPPLAVDKGTSVLALSEGCTTVAYLGDDLGDLPAFAALDQ
                     LAAAGVTTCKVAIASDELHDDVAAAADLVLDGPHAVLAALGPLA"
     gene            2331..3344
                     /locus_tag="VLR27_07005"
     CDS             2331..3344
                     /locus_tag="VLR27_07005"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HSP03232.1"
                     /translation="MRVVAAPDKFRGTASAAEVAAAVGRAAATAGWRCDEVPVADGGD
                     GLLDVLGGPNRTSVVTGPLGDPVEAGWRYERGTAVIEMARASGLLLAGGPEGNDPIAA
                     DTHGTGELIQTAVEAGATRVIVGLGGSATTDGGLGALRALYPHQRLRAVELLVACDVR
                     TRFLDAAEVFGPQKGASRAQVRLLTNRLARLADVYLEDHGVDVRELPGSGAAGGLAGG
                     LAAIGAQLLDGFALVAEERELDQAIEGADLVVTGEGFLDDASFEGKVVGGVAEMASDL
                     GVPVVAVVGEEIDAEAVHAAVRECGIAEVVSLVERFGRDRAMTDTVACVEQVVAEVLA
                     ARS"
     gene            complement(3356..3553)
                     /locus_tag="VLR27_07010"
     CDS             complement(3356..3553)
                     /locus_tag="VLR27_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03233.1"
                     /translation="MDDRTEPPTWTSGLVPKIVLAGIVVFVALTVIGWIVGAIIAVLR
                     TVAIVAVVIGVIWAVFAARRD"
     gene            3630..5003
                     /locus_tag="VLR27_07015"
     CDS             3630..5003
                     /locus_tag="VLR27_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSP03234.1"
                     /translation="MWERFAVAGRVAWSLLGVAAALALVFWIAWQFRVVFPPLVLAGA
                     IVFLLNPIVTGLQRKGFPRAAGTGLSYLGFIGILVLLGFLVAPLVSTQADELAEQWPE
                     LRADMEDWLDDLSQDSKDGNWIVEIPNVEELRDQFGNGNETAQNVEFDRIIERAAAEL
                     ERNEEPVLAADLDRVAADAREQFPEQGGIGEQLNRAREIGSRVFEVGLIFVLGPIIAF
                     YLLVDLPHIGAVSRRLIPRPASDQVLHVAHRLNVAIGGFFRGQLAVAVIVGIMVSIGL
                     AIIDLPFWLIVGMIAGAFNLVPLIGPWVGAVPGVIIALTTRDFSTALWVAAIMTIAQQ
                     IDNHFISPLVMQRTVSLHPSVVMLALLAGGTLGGFFGLILAVPVTATIKVVVGHLWAV
                     YVLEVPVEEVIAQDTGPPLGGHGPLEPLDDYVNDEIGVEEQVDHREVDARPADQAPIP
                     EGQGERR"
     gene            complement(4985..5872)
                     /locus_tag="VLR27_07020"
     CDS             complement(4985..5872)
                     /locus_tag="VLR27_07020"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HSP03235.1"
                     /translation="MSTLLRTSHGDFAPTDVAARKGDLTVAVCLPARDEEATVGTIVE
                     TLRHDLQEVVALVDELVVVDDHSEDATARVAQDAGARVVDARTTLPAVTGAGKGAALW
                     KSLHETTSDLVVWVDADITEFSSHFAAALIGTLVENPEVSFVKGAYDRPLVEGEGGGR
                     VTELVARPALAMLFPELTVVAQPLAGEYGGRRALLETLPFVQGYGVDIALLIDAHASV
                     GMAGLAEVDLGVRRHRNRTIGQLGPQGAEVLRAALVRAGVPTGDEHLTLRRPGLPPLA
                     LAHDELAPLVELDSYRRSP"
     gene            6013..6288
                     /locus_tag="VLR27_07025"
     CDS             6013..6288
                     /locus_tag="VLR27_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HSP03236.1"
                     /translation="MSATVRIPTTLRPMAGGKSEVEVEGATVGEVLKSLDAAHPGFSD
                     RLLDDEGNLRKFVNVFVADDDVRFMDGLQTPVPDGETVAIIPAVAGG"
     gene            6397..>7676
                     /gene="groL"
                     /locus_tag="VLR27_07030"
     CDS             6397..>7676
                     /gene="groL"
                     /locus_tag="VLR27_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799237.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HSP03237.1"
                     /translation="MAKMIAFDSEARRSLEAGMNKLADAVRVTLGPKGRNVVLDKKWG
                     APTITNDGVSIAKEIDLEDPYERIGAELVKEVAKKTDDVAGDGTTTATVLAWSMVREG
                     LRNVAAGANPMSLKKGIEAAVEAAVSSIKDQAVEVEGKGQIAQVASISSADEEIGTMI
                     SEAIDKVGKDGVITVEESQTFGMDMDLVEGMRFDKGYISPYFVTDQDRMEAVLDDAYV
                     LLVSSKITAVRDMLPVLEKVMQSGKPLAIIAEDIEGEALATLVVNKIRGTFKAVAIKA
                     PGFGERRKAMLQDMAVLTGGQVISEEIGLKLENTTLDLLGTAAKIVVTQDATTIVDGG
                     GTDDDLQGRIGQIKAEIDNTDSDYDREKLQERLAKLSGGVAVLKVGAATEVELKEKKH
                     RIEDAVSTTKAAIEEGVVAGGGVTLLRAQAKVLD"
BASE COUNT         1094 a         2819 c         2723 g         1040 t
ORIGIN      
        1 ccgacgcact ggacctggac ccgctcctcg tccgtcgcct gcgccgggcc cgggccgatc
       61 gccgtcgacg tcgctacgtc ggtccgaccc gccgccggag gctccgatga gcgaacagtc
      121 cgagcagccg cggcgacgcc gtcggcagcc acaacccaag tcgcctcccg cccaggggtc
      181 cgtcgcgcgc ggcgcgatcc tggtggcggt cgccgtcgtc atcggcgtgc tgctgctgcg
      241 agacgacgat tcggccacga cgcaggtcgc ggtcggctcc gacaccgcgg ctgaggtcga
      301 cgacaccgac gtggacgagt cgggggacga agaccccgat gccaccacca cgaccacgac
      361 cgaggcgccg cgcccgcccg ccgaggtgaa ggtgctcgtc gccaacggct ccggcgtgaa
      421 cggcgcggcc ggcggcacca ccgatgcgct cgaggcgctc ggctacgtca ccggcactcc
      481 ggccaacgcc gagcgcgtgc cggccacggt cgtgtacttc accaccggct tccaggccga
      541 ggccgaagcc ctcgccgccg ccatcggtgc gccggaggac ggcgtcacgc cgatgccggc
      601 cgtggcgccg gtcgacgacc tgcagctggc caacgtgctc gtgcacctcg gtcccgacct
      661 cgcggccggg taggcagccc gatcaggccg gcgtcaccgg gccgctgagc cggcggctgt
      721 gggtgacgat ccggtactcg atgtccccgc ggatcggctc ggcgtccacg ccggccggcg
      781 gttcggcgat gacctcgacg gtgcccacgg tgaccgtgcg gtcgcggttc atcgccccgt
      841 cgccgaggtc gtacgcggtc tccgacgtcg tgcgctgcgt cccgacgagg cgcacgcgcc
      901 cgtcgtcgct gtcgatgatc gtgcgcaccg gcacgtcgag ctcgaccacg gcgacggcga
      961 cgtcgcgccc gtcctccacg cccagggagt cgatgcggcc ccggccctgc accagcaccg
     1021 gacgtgacag gccgtcgagg ctcacctcgc gcaggatctc ccagcggtcc ccgggctcga
     1081 ggcgaccggt gggcgggctg gcgatgtcgg ccggcaggtc catggccacg tcgaggccga
     1141 gcgcctcggc cggcacgccc tcgacgaggt cgatggcggt gaggcggctg gcccggtcga
     1201 ggcggacgtc gacggtgcgc ggggccgcgt cgtcgcgctg cagcgcgacc cggaccagca
     1261 cctcgtcgtc ggtcaccgac tgcacggtct gggtggcgtc gagcgtggcg gcgtcgtcct
     1321 cgtcggtggt caccccgtcc accgtgcgga cgacctcggt ctcgacgtcg gcgcggaagc
     1381 ggtggacgtc gccgacctcg ggctcgaagc gcaccgccac ggtgtgctcc tcgcacgccg
     1441 ccagcgccag caccagggtg ctggcggcga cggcacgtcg gacgcggcgg gacgggacca
     1501 cggccgcagt gtgacagagg aggggaacta cggtcggacc gtgcccgacg ccctcgacgt
     1561 ccccctcacc ggtcgccgcg ccgaggacca gctgcgggcc gtggtcggca gggagccggc
     1621 ccgcacggcg ctgttcatcg acttcgacgg caccctcgcg ccgatcgtgc ccgacccggc
     1681 atcggccacc gcgatcgacg gcgcggtcga gctgctcgag cgactcggcc ggcgcttccg
     1741 gcgggtcgcc gtcgtcagcg gccggccacg ggccttcctg gtggaacggg tcggggccgg
     1801 cgtggacctc tcgggcgtgt acggcctcga gacccgcatc gacggcgtcg aagcggacca
     1861 cgacgaggcg caccgctggc gctccgtgat cgccgaggtg gcggcgagcg gcgccggtct
     1921 cccgtcggac gtgctcgtcg agtccaaggg gctgtcgctg accgtgcact accgggaggc
     1981 gcccgacgcc gaggccgagg tgctggcctg ggccgacgag gtcgcccgca ccgccggtct
     2041 cgaggtccgc gccgccaagc gctcggtgga gctgcatccg ccgctggcgg tcgacaaggg
     2101 cacctcggtc ctcgcgctga gcgaggggtg cacgacggtg gcctacctcg gcgacgatct
     2161 cggcgacctc cctgccttcg cggcgctcga ccagctggct gccgccggcg tcacgacgtg
     2221 caaggtcgcc atcgcgtccg acgagctgca cgacgacgtg gctgccgcag ccgacctggt
     2281 cctcgacggc ccgcacgccg tcctcgccgc gctcgggccg ttggcctgac gtgcgggtcg
     2341 tcgcggcacc cgacaagttc cggggcacgg cgtccgccgc cgaggtcgcc gctgccgtcg
     2401 ggcgcgccgc ggccaccgcc ggatggcgct gcgacgaggt gccggtggcc gacggcggcg
     2461 acgggctgct cgacgtgctg ggtggaccga accgcacatc ggtcgtcacc gggccgctcg
     2521 gcgacccggt cgaggccgga tggcgctacg aacggggcac ggccgtcatc gagatggctc
     2581 gggcgtccgg cctgctcctg gcgggcggcc ccgagggcaa cgacccgatc gccgccgaca
     2641 cccacggcac cggcgagctg atccagaccg cggtggaggc cggcgccacc agggtcatcg
     2701 tggggctggg cggctccgcc accaccgatg gcggcctcgg cgcgctccgc gccctctacc
     2761 cgcaccagcg cctgcgcgcc gtcgagctgc tggtcgcctg cgacgtgcgc acccggttcc
     2821 tcgacgccgc cgaggtcttc gggccccaga agggcgccag ccgggcgcag gtgcgcctgc
     2881 tcaccaaccg cctcgcccgt ctcgccgacg tctacctgga ggaccacggg gtcgacgtgc
     2941 gggagctgcc gggctcgggt gcggccggcg gtctcgccgg aggtctcgcc gccatcgggg
     3001 cgcagcttct cgacgggttc gccctcgtcg ccgaggagcg cgagctggac caggccatcg
     3061 agggtgccga tctggtcgtc accggcgagg gcttcctcga cgacgcctcc ttcgagggca
     3121 aggtcgtcgg cggcgtggcg gagatggcgt ccgacctcgg agtcccggtg gtggccgtcg
     3181 tcggcgagga gatcgacgcc gaagccgtgc acgccgcggt tcgggagtgc gggatcgccg
     3241 aggtcgtgtc gctcgtggag cgcttcggcc gggaccgggc catgaccgac accgtcgcct
     3301 gcgtcgagca ggtcgtcgcc gaggtgctgg ccgcccggag ctgaggcgcc ggggctcagt
     3361 cgcggcgagc ggcgaacacg gcccagatca cgccgatcac cacggcgacg atggcgacgg
     3421 ttcgcagcac ggcgatgatg gcgccgacga tccagccgat gaccgtgagg gcgacgaaca
     3481 ccacgatgcc agccagcacg atcttgggca cgaggcccga ggtccacgtc ggcggctcgg
     3541 tgcggtcgtc catggcacga gggtaggtcc cgacggcggt ccgtgccggc ggccaccggt
     3601 ctcgaggggg gtccggtacc gttcggccga tgtgggagcg attcgccgtc gcaggccggg
     3661 tggcctggtc gttgctcggc gtcgccgcgg ccctcgcgct cgtgttctgg atcgcgtggc
     3721 agttccgggt cgtgttcccg cccctggtgc tcgccggcgc catcgtcttc ctgctcaacc
     3781 ccatagtcac cggcctgcag cgcaaggggt tcccccgcgc cgccggcacc ggcctcagct
     3841 acctcggctt catcggcatc ctcgtgctgc tcggcttcct cgtcgcgccg ctcgtcagca
     3901 cccaggccga cgagctggcc gagcagtggc cggagctgcg agccgacatg gaggactggc
     3961 tcgacgacct gtcccaggac tccaaggacg gcaactggat cgtcgagatc cccaacgtcg
     4021 aggagctgcg ggaccagttc ggcaacggca acgagaccgc gcagaacgtc gagttcgacc
     4081 ggatcatcga gcgggcggcg gccgagctcg agcgcaacga ggagccggtg ctcgccgccg
     4141 acctcgaccg cgtcgccgcc gacgcccgtg agcagttccc cgagcagggc ggcatcggcg
     4201 agcagctgaa ccgggcccgc gagatcggca gccgcgtctt cgaggtgggc ctcatcttcg
     4261 tgctgggacc gatcatcgcc ttctacctgc tggtcgacct gccccacatc ggtgcggtgt
     4321 cgcgccgcct catcccccgc cccgcgagcg accaggtgct ccacgtcgcc caccggttga
     4381 acgtggccat cggcggcttc ttccgcggcc agctggcggt ggccgtgatc gtcgggatca
     4441 tggtgagcat cggcctggcg atcatcgacc tgccgttctg gctcatcgtc ggcatgatcg
     4501 ccggcgcgtt caacctggtg ccgctcatcg gcccgtgggt cggtgcggtg cccggcgtga
     4561 tcatcgccct caccacccgg gacttcagca cggcgctgtg ggtggcggcg atcatgacca
     4621 tcgcccagca gatcgacaac cacttcatca gcccgctcgt catgcagcgc acggtgagcc
     4681 tgcacccgtc ggtcgtcatg ctggccctgc tcgctggcgg cacgctgggc ggcttcttcg
     4741 gtctgatcct ggccgtcccc gtcaccgcga ccatcaaggt ggtcgtcggg cacctgtggg
     4801 ccgtctacgt gctcgaggtg ccggtcgagg aggtcatcgc tcaggacacc ggtccaccgc
     4861 tcggcgggca cggacccctg gagcccctcg acgactacgt gaacgacgag atcggggtcg
     4921 aggagcaggt cgaccaccgc gaggtggacg cgcgcccggc agaccaggcg ccgatccccg
     4981 aaggtcaggg cgagcggcgg taggagtcca gctccaccaa cggtgcgagc tcgtcgtggg
     5041 cgagcgccag cggcggcagg ccgggacgcc gcagcgtcag gtgctcgtcg cccgtgggca
     5101 cgcccgcccg cacgagcgca gcgcgcagca cctcagcgcc ctgcgggccg agctggccga
     5161 tggtgcggtt gcggtgccgg cgcacgccga ggtcgacctc ggccaggccg gccatgccga
     5221 ccgaggcgtg agcgtcgatg agcagggcga tgtcgacccc gtagccctgg acgaagggga
     5281 gggtctcgag cagcgcccgc cggcccccgt actcgccggc cagcggctgg gcgacgacgg
     5341 tgagctcggg gaacagcatc gccagcgcgg gccgggcgac cagctcggtg acccgtccgc
     5401 cgccctcgcc ctcgacgagg ggccggtcgt aggccccctt cacgaacgag acctccgggt
     5461 tctccaccag cgtgccgatg agggcggcgg cgaagtggga gctgaactcg gtgatgtcgg
     5521 cgtccaccca caccaccagg tcgctcgtgg tctcgtgcag cgacttccac agcgccgcac
     5581 ccttgccggc gccggtgacc gccgggagcg tggtgcgggc atcgaccacg cgggcgccgg
     5641 cgtcctgcgc gacccgcgcc gtggcgtcct cggagtggtc gtcgaccacg accagctcgt
     5701 cgacgagcgc cacgacctcc tgcaggtcgt ggcgcagggt ctccacgatc gtcccgaccg
     5761 tggcctcctc gtcccgggcc ggcaggcaca ccgccaccgt caggtcgccc ttgcgggccg
     5821 cgacgtcggt cggggcgaag tcgccgtggg acgtgcggag cagggtcgac acgtcgagca
     5881 gtgtcgtccc gggccggtac cgccgcaaag ccgcgatcgc cggggaatgc ctgccgcagg
     5941 tgactcgtta gcatcgaaga gccggtccga ccggccccgt cgccaccccc gaccctgagg
     6001 aaaccccctg tcatgagcgc caccgtccgc atccccacca ccctccgccc catggccggc
     6061 ggcaagtccg aggtcgaggt cgagggcgcc accgtcggcg aggtgctgaa gtccctcgac
     6121 gccgcccacc ccggcttctc cgaccgcctg ctcgacgacg agggcaacct gcgcaagttc
     6181 gtgaacgtgt tcgtggccga cgacgacgtc cgcttcatgg acggcctgca gaccccggtg
     6241 cccgacggcg agaccgtcgc catcatcccc gccgttgctg gcgggtgagc gcagcgttcc
     6301 gttagcagtc tgatgttgcg actgctaacg gaacgtgctt ggctgttagc agtctctgtc
     6361 taggactgct aaccgccgag gaaagaaggt cgacacatgg ccaagatgat cgcgttcgac
     6421 agcgaagccc gccgctcgct cgaggcaggc atgaacaagc tcgccgacgc cgtccgagtc
     6481 acgctcggcc ccaagggccg caacgtcgtg ctggacaaga agtggggcgc ccccaccatc
     6541 accaacgacg gcgtctccat cgccaaggag atcgacctcg aggaccccta cgagcgcatc
     6601 ggcgccgagc tggtcaagga ggtcgccaag aagaccgacg acgtcgccgg tgacggcacc
     6661 accaccgcca ccgtcctcgc ctggtcgatg gtccgcgagg gcctgcgcaa cgtggccgcc
     6721 ggcgccaacc ccatgtccct gaagaagggc atcgaggccg ccgtcgaggc cgccgtctcc
     6781 tccatcaagg accaggccgt cgaggtcgag ggcaagggcc agatcgccca ggtcgcctcc
     6841 atctcctcgg ccgacgagga gatcggcacc atgatctccg aggccatcga caaggtcggc
     6901 aaggacggtg tcatcaccgt cgaggagagc cagaccttcg gcatggacat ggacctcgtc
     6961 gagggcatgc gcttcgacaa gggctacatc tcgccgtact tcgtgaccga ccaggatcgc
     7021 atggaggccg tcctcgacga cgcctacgtc ctcctcgtgt cctccaagat cacggccgtg
     7081 cgcgacatgc tcccggtgct cgagaaggtc atgcagtcgg gcaagccgct cgcgatcatc
     7141 gccgaggaca tcgagggcga ggccctcgcc accctcgtcg tcaacaagat ccgcggcacg
     7201 ttcaaggccg tggccatcaa ggccccgggc ttcggcgagc gccgcaaggc gatgctgcag
     7261 gacatggccg tcctcaccgg tggccaggtc atctccgagg agatcggcct caagctcgag
     7321 aacaccaccc tcgacctgct cggcaccgcc gccaagatcg tcgtcaccca ggacgcgacc
     7381 accatcgtcg acggtggcgg caccgacgac gacctgcagg gtcgcatcgg ccagatcaag
     7441 gccgagatcg acaacaccga ctccgactac gaccgcgaga agctccagga gcgcctggcc
     7501 aagctgtccg gcggcgtcgc cgtcctgaag gtcggcgcag ccaccgaggt ggagctcaag
     7561 gagaagaagc accgcatcga ggacgcggtg agcaccacca aggccgccat cgaggaaggt
     7621 gtcgtcgccg gtggtggcgt caccctgctc cgggcccagg ccaaggtgct cgacat
//
LOCUS       DATISU010000172         4328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_520511, whole genome shotgun sequence.
ACCESSION   DATISU010000172 DATISU010000000
VERSION     DATISU010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520511"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VLR27_07035"
     CDS             <1..1114
                     /locus_tag="VLR27_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HSP03238.1"
                     /translation="IGTTDKGELVEINKRAATSDLIVYVNINLVSMDGGWKSTATGLS
                     GYRSLRHHHNVHTMQHSKSFMDRHASELHHSNWRMGKVLRDGGVKVFQVETTLNNDTF
                     GTSALDTASPLSMLQKREWEWTAKDRATFMAVQAGLKRMPARAKRAAFQSWKAPYAVT
                     SVQAGEVEAVHERTLEQNFAQHLVPVEGQTDVLTMGLPYICPYNVNSVMNPILVMCLG
                     LGYFFNMYRGKPPVREGGVLIMRHPTPWEFHPVHHPSYIDFFEQVLSQTTDPVEVEKY
                     EQAFAEDEWYRHLYRTSYAYHGVHPFYMWYWGSHALQHLGKVVIVGGDPKAVRRLGFA
                     PASTMADALEIASDVVGPAPTITHVHNPPLLMADVT"
     gene            1125..2066
                     /locus_tag="VLR27_07040"
     CDS             1125..2066
                     /locus_tag="VLR27_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSP03239.1"
                     /translation="MSLLDRLGVELPDPRRKLRQLAFPLVAPTTPRGVEPRAKERRTG
                     ADYDTTWARTFRARWTRTVLLESVLRPTAAAIARPRRRGIDRLADLQDAGGPVIFCAN
                     HHSHVDTPIVLTSLPEPWRYQVFVGAAADYFFRNRATGAAASLLLNAIPIERATVTRR
                     SADLAAELIDDGWSMLIFPEGGRSPDGWGQDFRGGAAYLARRTGAPVVPIHLSGTDRI
                     LPKGAKKLRPGPTMVTFGDPIRAEEGEDTRRLATRIEAAVAALADEHTSDWYSARVRA
                     HTGDTPGLGGPDGPSWRRAWALGDRSPRRRRKPTWPN"
     gene            2197..3108
                     /locus_tag="VLR27_07045"
     CDS             2197..3108
                     /locus_tag="VLR27_07045"
                     /inference="COORDINATES: protein motif:HMM:NF037405.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HSP03240.1"
                     /translation="MPEVLGAAARILAAQQSLITTRQAASVGIDRERCRSLVHQQIWE
                     RIDQGVYGPLGVPLTWKRQLMAALLLAPDGSLGSHRAAATVLGVGGIPEPVPELTIPR
                     GTTFRRPWLVVHESTDLALADRRRIDGIPVTGPRRLAMDLGSVVSEKRYRQTMRELRS
                     RHGVGFQDLLRTYLRHKRSGRNGGAALRDWLDRYVDVGGMPESALEQVALDALLDAGL
                     HPVAQLWVEVAGGARYRLDLAFPDRMIAVEVDGSQHEERPAMEADALRDAALEALGWT
                     VIRIRSKTFTADLARAIQILVDQSVVG"
     gene            complement(3129..3782)
                     /locus_tag="VLR27_07050"
     CDS             complement(3129..3782)
                     /locus_tag="VLR27_07050"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSP03241.1"
                     /translation="MREIRYVAIADDLRRQITAGDLVAGAVLPSEADLAGSYGVSRVT
                     VRKALEVLREEGLIGSRQGFGWFVAAPMVRRSLGTLSTIEADLAAQGREPMRKVLSFG
                     FRDGELEVVRVNLADGQPFALVTVWCPEELGRSLSRDDVERSTFYDLLPVDLVSVDQT
                     IRAAAADERDAEVLGIPPGSPLLVALRISRDAAGEPFLRSEHRFPAHLTEFVLEGGP"
     gene            3801..>4328
                     /locus_tag="VLR27_07055"
     CDS             3801..>4328
                     /locus_tag="VLR27_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HSP03242.1"
                     /translation="MPVTSSTPIELVQAVYDRLPDSVRIGRERLGRPLTLAEKVLINH
                     LRDREGQEMERSRSYADFDPDRVAMQDATAQMALLQFMTAGLDEAAVPSTVHCDHLIQ
                     AKVGALKDLEVATETNHEVYEFLETVSRRYGIGFWKPGSGIIHQVVLENYAFPGGMMI
                     GTDSHTPNAGGLGMVA"
BASE COUNT          675 a         1654 c         1393 g          606 t
ORIGIN      
        1 gatcggcacc accgacaagg gcgagctggt cgagatcaac aagcgggccg ccacgtcgga
       61 cctcatcgtc tacgtgaaca tcaacctcgt gtcgatggac ggcggctgga agtccaccgc
      121 caccggcctg tccggctacc ggtcgctgcg ccaccaccac aacgtccaca ccatgcagca
      181 ctcgaagagc ttcatggacc gccacgccag cgagctgcac cacagcaact ggcgcatggg
      241 caaggtgctg cgcgacggcg gcgtgaaggt cttccaggtc gagaccacgc tcaacaacga
      301 cacgttcggc acgtccgccc tcgacaccgc cagtcccctc tccatgctgc agaagcgcga
      361 gtgggagtgg acggccaagg accgggccac gttcatggcc gtccaggccg ggctcaagcg
      421 catgccggcc cgggccaagc gggccgcgtt ccagtcgtgg aaggcgccct acgccgtcac
      481 ctccgtgcaa gccggcgagg tcgaggccgt gcacgagcgc acgctcgagc agaacttcgc
      541 ccagcacctc gtgccggtcg agggccagac cgacgtgctc accatgggcc tgccctacat
      601 ctgcccgtac aacgtgaact cggtgatgaa cccgatcctg gtcatgtgcc tgggcctcgg
      661 gtacttcttc aacatgtacc gcggcaagcc gccggtgcgc gagggcggcg tgctgatcat
      721 gcgccacccc acgccgtggg agttccaccc agtgcaccac ccgagctaca tcgacttctt
      781 cgagcaggtg ctgtcccaga ccaccgatcc cgtcgaggtc gagaagtacg agcaggcctt
      841 cgccgaggac gagtggtacc gccacctgta ccggacgagc tacgcctacc acggcgtcca
      901 cccgttctac atgtggtact ggggcagcca cgccctccag cacctgggca aggtcgtcat
      961 cgtcggtggc gaccccaagg ccgtgcgccg cctcggcttc gcgcccgcct cgaccatggc
     1021 cgacgcgctg gagatcgcat ccgacgtcgt cggccccgcc cccaccatca cccacgtgca
     1081 caacccgccc ctgctcatgg cggatgtcac atgagtcgag cccgatgagc ctcttggacc
     1141 gcctgggcgt ggagctgccg gacccgcggc gcaagctccg gcagctggcg ttccccctcg
     1201 tcgcgccgac gacgccccgt ggcgtggagc cgcgagcgaa ggagcgtcgc accggcgccg
     1261 actacgacac cacctgggcc cgcaccttcc gcgcccgctg gacccgcacc gtcctgctcg
     1321 agtccgtcct gcgtcccacc gccgccgcca tcgctcgacc gcgacgccgc ggcatcgacc
     1381 gcctcgctga tctccaggac gcgggcggcc cggtcatctt ctgcgccaac caccacagcc
     1441 acgtcgacac gcccatcgtg ctcacgtcgc tgcccgagcc gtggcgctac caggtgttcg
     1501 tgggcgccgc cgccgactac ttcttccgca accgggccac gggcgcagcc gcctcgctgc
     1561 tgctgaacgc catcccgatc gagcgggcca ccgtcacccg ccgctctgcc gacctcgccg
     1621 ccgagctcat cgacgacggc tggtcgatgc tgatcttccc cgagggcggg cgctcccccg
     1681 acggctgggg ccaggacttc cgcggcggcg ccgcctacct ggcccgccgc accggtgcgc
     1741 ccgtcgtgcc gatccacctg agcggcaccg atcgcatcct gcccaagggc gccaagaagc
     1801 tccgtcccgg gcccaccatg gtcaccttcg gcgacccgat ccgggccgag gagggtgagg
     1861 acacccgtcg cctcgccacc cgcatcgaag cagcggtcgc cgccctcgcc gacgagcaca
     1921 cctccgactg gtactcggcc cgcgtccggg cccacaccgg cgacaccccc ggcctcggcg
     1981 gccccgacgg ccccagctgg cgccgggcct gggccctcgg cgaccgctcc ccccgccggc
     2041 gccgcaagcc cacctggccg aactagcccc caaccggaat ttcgtggatg agtcaggcgt
     2101 gacctgactg atccacgaca gacgcacgct cccccggtcg ttgcctacga tcgacgccgg
     2161 cttcccccgc cgacccgtcc accgggaggg aagcccgtgc ccgaagttct gggcgctgcc
     2221 gcacgcatcc tcgctgcgca gcagagcctc atcaccacac gtcaggccgc atctgtcggc
     2281 atcgaccggg agcggtgccg cagcctggtc caccagcaga tctgggagcg gatcgaccag
     2341 ggcgtctacg gaccgctcgg ggtgcccctc acctggaagc gacagctcat ggccgccctc
     2401 ctgctggcac ctgacgggtc gctgggttcg catcgagcgg cagcgaccgt gctgggcgtg
     2461 ggaggcatcc ctgaacccgt gccggagctc acgattcccc ggggcacgac cttccgtcga
     2521 ccctggctgg tcgtgcacga atcgaccgac ctcgccctcg ccgaccggcg ccggatcgac
     2581 ggcatcccgg tgaccggtcc ccgtcggttg gccatggacc tcggatcggt ggtgtcagag
     2641 aagcgctacc gccagaccat gcgcgagctc cgctctcgcc acggcgtcgg cttccaggac
     2701 ctgctgcgca cctacctccg gcacaagcgc tccggccgca acgggggtgc cgccctacgg
     2761 gactggctgg accggtacgt cgacgtcggc ggcatgccgg agtccgcgct cgagcaggtc
     2821 gcgctcgacg cgctcctcga cgccggactg cacccggtcg cccagctgtg ggtcgaggtc
     2881 gctggcggcg cccgctaccg cctcgatctg gccttcccgg atcggatgat cgcggtcgag
     2941 gtcgacgggt cccagcacga ggaacgccca gcgatggagg cagacgcgct ccgggacgca
     3001 gcgctcgagg cgctcgggtg gacggtcatc cgcatccggt ccaagacctt cacggccgat
     3061 ctcgcccggg cgatccagat tctcgtggat cagtcagtcg tcggctgacc catccacgaa
     3121 attcgtggct acggcccgcc ttccaggacg aactcggtga ggtgagcggg gaagcggtgc
     3181 tcggagcgga ggaacggttc gccggcggcg tcgcggctga tccggagcgc gaccagcagc
     3241 ggtgagcccg gggggatgcc gaggacctcg gcatcccgct cgtctgcggc cgcggcgcgg
     3301 atcgtctggt cgacgctgac gaggtcgacg gggagcaggt cgtagaaggt ggaccgctcg
     3361 acgtcgtcgc ggctgagcga gcggcccagc tcctcggggc accacaccgt cacgagcgcg
     3421 aagggttgcc cgtcggccag gttcacccgc accacctcga gctcgccgtc gcggaatccg
     3481 aacgacagga ccttgcgcat gggctcccgg ccctgggcgg cgaggtcggc ctcgatcgtg
     3541 gacagcgtgc cgagcgaccg gcgcaccatc ggggcggcga cgaaccaccc gaagccctgc
     3601 cgcgacccga tgagcccctc ctcgcgcagc acctcgaggg cctttcggac cgtgacgcgg
     3661 ctcaccccgt aggatccggc caggtcggcc tccgagggca gcacggcccc ggcgaccagg
     3721 tcgccggccg tgatctggcg acggaggtcg tcagcgatcg ccacgtatcg gatctctctc
     3781 acttgtatta gagttgcatc atgcctgtca cttcgtccac ccccatcgag ctcgtccagg
     3841 ccgtctacga ccggttgccc gactccgtcc gcatcggtcg tgagcgcctc ggccggcccc
     3901 tgaccctcgc cgagaaggtc ctgatcaacc acctccggga ccgcgagggc caggagatgg
     3961 agcggagccg cagctacgcc gacttcgatc cggaccgggt ggcgatgcag gacgccaccg
     4021 cccagatggc gctgctgcag ttcatgaccg ccggcctgga cgaggccgcg gtcccctcca
     4081 ccgtgcactg cgaccacctg atccaggcca aggtcggcgc gctcaaggac ctcgaggtcg
     4141 cgacggagac caaccacgag gtgtacgagt tcctcgagac cgtcagccgc cgctacggca
     4201 tcgggttctg gaagccgggc tcgggcatca tccaccaggt cgtgctcgag aactacgcct
     4261 tcccgggcgg gatgatgatc ggcacggaca gccacacccc caacgccggc ggcctgggca
     4321 tggtcgcc
//
LOCUS       DATISU010000173        27301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_893015, whole genome shotgun sequence.
ACCESSION   DATISU010000173 DATISU010000000
VERSION     DATISU010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 27301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27301
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893015"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1558
                     /locus_tag="VLR27_07060"
     CDS             17..1558
                     /locus_tag="VLR27_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSP03243.1"
                     /translation="MDFIQDWGLTAMVFAPLAGVAIMMLFPKDADDTHKVLALLTSLV
                     TAAIGIAVLADFDYDDTGSLQFLRDNEWISVINSRFILGIDGLSVPLIALTLLITPLV
                     IAYSWNHFPEPHNPKAFLSLILVLHTGMLGTFVAQDLILFFVFFEVVLLPMYFMIGVW
                     GGPERQYASIKFFLYTLFGSALMLLSFLALFFLTGGETFSIVELAERVQGMDIARTTQ
                     LIVFGGMFMGFAIKVPMFPFHTWLPDAHTQAPTQGSVILAAVLLKLGTYGFIRVALPI
                     LPEAAQSWAPWIGGLAVIGIIYGALGCLAQTDMKRLIAFSSVAHMGYTMLGISTLTDF
                     GLNAAVFGMVAHGLITGMLFFIAGSVKERYHTLEIKRLGGLLVSAPRLGWILGFCTMA
                     SLGLPGLAGFWGEFPAILAAYQPAEGLPVELFRGYMVVAAIGTVLAAGYLLWLYQRTA
                     FGTVKDEFVDDPNIHDVNGPEWIAWTPLLALILLLGVYPNLLFKVSDGSIVNVAQQIA
                     AATGN"
     gene            1572..3053
                     /locus_tag="VLR27_07065"
     CDS             1572..3053
                     /locus_tag="VLR27_07065"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HSP03244.1"
                     /translation="MLAQVSDFVAPHIDFHAFGPELALVGALVAVMVVDLIKPDGNNP
                     FLSSIAGLGVLGAMIPVITLATDGADRFLFDGAYVVDNFSLVLKALFLVSGYLVILMS
                     TNYIAEGDYAEGEYYLLLLSSLLGMILMASARDLITVFIALELLSIPAYILAGWRKRD
                     LMGNEAGVKYYLMGVFASAVLLYGMSLLFGVAGDTRLTEIGQAIAGGAGETPLITLAV
                     VFVVVGFAFKVSAVPFHTWAPDVYQGAPTPITAFLAVASKAAGFVALLQVIYLAFPAR
                     DDIIQPLMWVLAAATMTIGNLIALRQTNVIRLLAYSGVAQAGFILAPLSVDSDLSLQA
                     VITYLVIYAAMNLGAFAAVIGIARKTRSGEISSFGGLFEYAPGLTVILSLFLFSLAGI
                     PPLGGWLAKFVVFRALADANTASGYVMGAIVAVNSVIALYYYANVAREMWMKPVPDGD
                     RSPVVVPFSISAALALTVVATVAFGVSNLATRLGDQATFLLAL"
     gene            3064..3990
                     /locus_tag="VLR27_07070"
     CDS             3064..3990
                     /locus_tag="VLR27_07070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HSP03245.1"
                     /translation="MTGEPVHTFVERALYGSDGFYEQGGRAGRRGASFVTSPEVGPLF
                     GAVLASALDRWWDDAGRPDEWVVVDAGAGPGTLARAVLAAQPACAAALRLVCVERTSA
                     QRELHPGGVTSTDRLPDVADVIVANELLDNLAFDLLERTDAGWVPIEVRADGSTQPDT
                     AGPRIPVAGDACAWVTTARRRLRPHGRLVCFDYADTTAALAARPWTDWVRAFAGHEQV
                     ADPFAQPGTRDVTVVVPWDQLPTPERSTDQATWLREHGIDDLVAEGIAHWEAHAAAPD
                     LEAMRMRSRVREAEALIDPAGLGGFWVGEWRP"
     gene            4067..5002
                     /locus_tag="VLR27_07075"
     CDS             4067..5002
                     /locus_tag="VLR27_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP03246.1"
                     /translation="MTPVIRCSSLVKSFGATRALDGLDLEVAPGEVHGFLGPNGAGKT
                     VTMRIVLGLLRADAGEVTLLGGDPWHDAVALHRRLAYVPGDTNLWPNLTGGEIIDVLG
                     GLRGGLDDGRRAALVDRFDLDPRKKARTYSKGNRQKVALVAALASDAELLVLDEPTSG
                     LDPLMEAVFQDCILDARAEGRTVLLSSHILAEVEKLCDRVTIIRRGATVQTGTLAELR
                     HLTRTTITAEVREQPTALDELAGVHDLVVDGHRASFDVDSDHLDEAVDRLAALGVTSL
                     TSHPPTLEELFLRHYGDELADVGVDEPVARADGHP"
     gene            5002..6597
                     /locus_tag="VLR27_07080"
     CDS             5002..6597
                     /locus_tag="VLR27_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03247.1"
                     /translation="MVTLTGLGRLVRLVLRRDRVRLPVWVLGLAGVVIGSGASLVPLY
                     PEQADIQQYVDLFGDNPALVAFAGPGYGFGDPNLGVILVNETQLWACIGVALMSIFLV
                     NRHTRGEEEAERTEVLRSSVVGRHAPTAAAVVVVAAANIALAALCGIAFVALGYGVTG
                     SLAYAAGLAGCGLVFTGLTAVLAQLAGSSRATLGLATAVLGAAFVLRAVGDITGNALR
                     WLSPIGWAQGVRAYADEQWWPVALCLAVTVLAVAGAFWLATRRDLGSGILPQRPGPAR
                     AGWWATSPLGLAVRLQRGAVVAWMAGLVLMGGVYGSIGEDVEQMIEENETFGDVLAQL
                     DGADVTDAFFATAVSMQALLAAGFAISAALRIRSEEAAGRVEPILAGPVGRWRWAWSH
                     LLVAAVGTVLVVAAGGVGVGASYAVVSGDAGQVLRLTGAALATVPAVLVLAGVAVALF
                     GASLRLALVAWAGLGVVVLVELFGELLRLPSWSRMISPLHHVPGVPAEDLRAVPLLVL
                     TAIAVALTAVGMARFRSRDLQVG"
     gene            complement(6674..7228)
                     /locus_tag="VLR27_07085"
     CDS             complement(6674..7228)
                     /locus_tag="VLR27_07085"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HSP03248.1"
                     /translation="MRTSTRVLLMTIAMLGVLVAACGDDDETATTDTTTTEATTTSEP
                     DSTEAPTTFDVYLLPAETGNDCSVVIPSEREALEGDVDDALTQLLAGPTAEEQAQDLR
                     SWFSAETAGMLNGLTVEDGVAAVDFADFSGIIPNASSSCGSAGLLAQLDNTVLQFPQV
                     DEVVYSFDGDRAAFYEWLQLAPPG"
     gene            7427..7624
                     /locus_tag="VLR27_07090"
     CDS             7427..7624
                     /locus_tag="VLR27_07090"
                     /inference="COORDINATES: protein motif:HMM:NF015827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HSP03249.1"
                     /translation="MLAPGCGDDDDGEPGIANPASEFCEEQGGRVEMDRDGAGNERGM
                     CVLPDGTRVDEWEYYREQGGE"
     gene            complement(7637..7972)
                     /locus_tag="VLR27_07095"
     CDS             complement(7637..7972)
                     /locus_tag="VLR27_07095"
                     /inference="COORDINATES: protein motif:HMM:NF040323.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HSP03250.1"
                     /translation="MSQEVIPILRVASIDQSVEWYAQLGFHKEWEHRIEPDQPGFASI
                     ARGRGHIYLSEHDDDASPDSLVYIRHSQLDDLARRLGQELTEVPWGRELKVEDPDGNR
                     LRISDPIDD"
     gene            8035..9165
                     /locus_tag="VLR27_07100"
     CDS             8035..9165
                     /locus_tag="VLR27_07100"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HSP03251.1"
                     /translation="MRTAVTERLGIDFPILAFSHCRDVVAAVTNSGGMGVLGAVAHSP
                     KQLEIDLDWIAAETKGKPFGVDLLIPQKFVGADDGGLEVGSLRDLIPAEHREWLDGLL
                     AQNGIPPLPDDDAESVRSYGGMRVDPKSMAPLVDISFAKGVSLMASALGPPPPWLIER
                     AKAEGEVPVAALAGSLEHAIRHDQAGADLIIAQGTEAGGHTGEVATMVLVPEVVDAVQ
                     APVLAAGGIGRGRQIAASLALGAEGVWCGSVWLTTQEAETSDVIKDKFLAAPSSGTVR
                     SRSLTGKPARMIRTDWTDAWESEPSPGPLGMPLQSLLVAEAQSRIARVAAKDEGARRL
                     STYFVGQIVGSMNTVQPAARVVLDMVEEFIDAVERLDGLLQD"
     gene            9184..10089
                     /locus_tag="VLR27_07105"
     CDS             9184..10089
                     /locus_tag="VLR27_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSP03252.1"
                     /translation="MPSELLRTDQVTIGGVPTRAVRADGDPDRVRFLFLHGFSDSADC
                     WQRVQKRLAAAGHGSLAVDQPSHGHADPLHPDLPVIEQFLAFAAAAARATDDGRPVVV
                     VGNSLGGAHALLLGQHHPELVHGVAAISPAAFDHPGWFRILDSRFNVSPEGVVPTVVR
                     RALAGPAMRAVGFGMPHRVPAGFVRTWAQSWHDPTRRRALHDLATRVPAEYFHADPVD
                     LCAVDVPVLALWGTRDRLTLISSRQRFEQDCTDLEFVALPGVGHMAQVEVPGRTTKHL
                     LSFAARLTDGATPAGATEATLVASA"
     gene            10171..11412
                     /locus_tag="VLR27_07110"
     CDS             10171..11412
                     /locus_tag="VLR27_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP03253.1"
                     /translation="MTATTTAPGALSPDEAAAFRQRCIEFLDEHATGIHLDGDPDPRA
                     EKRLRAAQAFQQALADAGLAGITYPAEYGGAGLTKEHDKIWRECYAQYPFMTSELTIS
                     HGMCLPMLAEYGTEDQKQRYLADNIAARTVWCQMFSEPGAGSDVASLQSRAVLDGEEW
                     ILNGQKVWTTLAHLCDFGIVIARTDPDQPKHAGISMFIVDMRAPGVEIRPIHQVDGGL
                     HFNEIFFTDVRIPAENLVGDLNNGWRLATAMLMYERVAIGGGGTDGVTHANADALILE
                     AEKRGVLGDPALRNDLMDLYIQESVKSMNSLRTRAELKAGKTPGPAGSLGKLQGTLVS
                     YLARDLSMRVVGADSIAWGEEGGHRWSTQAIRSMGSGIAGGTNEIQKNIIGDRVLGLP
                     REPSVDKDVPFKDLRVGTQRD"
     gene            complement(11413..12036)
                     /locus_tag="VLR27_07115"
     CDS             complement(11413..12036)
                     /locus_tag="VLR27_07115"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HSP03254.1"
                     /translation="MALRVVGSGLGRTGTRSLKDALELLLGEPCYHMMECFPRPEHPA
                     RWTAAIRGAPTDWDDLFGGFAATVDWPSAACWKELAEAYPDALILHSERPADEWFRSA
                     DQTILETFKKPRDEWPAPGTDAWWDMAVTMFEDFTPDFLDRDALMAAVEAWNADVRAS
                     APADRLLIWQTGDGWEPICEALRVPVPDEPFPHINTTEQFRKGTGLE"
     gene            complement(12052..12690)
                     /locus_tag="VLR27_07120"
     CDS             complement(12052..12690)
                     /locus_tag="VLR27_07120"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HSP03255.1"
                     /translation="MPSPDSPLLFALGLPAGIVLWSFMEYVLHRFAFHEARGKNYGSR
                     EHLRHHGSEDTVLESWYLSWTGVLLVSLGLIPFLGRLAGTADLGWGVGIGYLVAYGFY
                     DLVHWRAHRRPYANRYEHMVRKHHFIHHFHAPLKNHGVTTPFWDHVFGTYVEVDVVRV
                     PRRMAMRWMLDEHGEVLPEYRGTYELRGSRPLDDDQREQDRELAFANQAPTL"
     gene            complement(12690..13478)
                     /locus_tag="VLR27_07125"
     CDS             complement(12690..13478)
                     /locus_tag="VLR27_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP03256.1"
                     /translation="MVQLSAVPDPHAVPVAPVGARERNRLARHRSYLRAALSLVFAEG
                     LDALTMQRLAQEVGAAVGSVYTYFPSKGALVAEVQREAIERLATSAALLGADVDRTID
                     GVEADAAHLAPVVAFGRFWLTAAEAYPEEMQLLLLLLSDVTESVPPEEAGRVVPAAMR
                     LLGLAESRLAAAARTGALDESQPADRRTIALAAALTGCVQLRVVQRWDDELLDPTATG
                     RHVLDALLHGWGAAPGPLTAAHEIVDDLEATGPLARPIPEGTDP"
     gene            13557..13922
                     /locus_tag="VLR27_07130"
     CDS             13557..13922
                     /locus_tag="VLR27_07130"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HSP03257.1"
                     /translation="MDFDDVIRIGSELPEVEVSTSYGTPALKVRGKAFCRLWGDDERE
                     RKGPEVDGDVLVVFVDVAEKRAWIQTSDGALFDQAHYHGHGAMLVKLDEVDEDLLLDV
                     LEQSYRIKAPRTLLRRLDD"
     gene            complement(13930..14586)
                     /locus_tag="VLR27_07135"
     CDS             complement(13930..14586)
                     /locus_tag="VLR27_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HSP03258.1"
                     /translation="MTSAADTFDLDQVDRLLTTTKQVRKRMDLERPVPRELLLECIDI
                     AQHAPMGGNLERNRWLVVDDPDIKAQIAPLYQQVGRPYLAANAAGEVDEGQQRVLDSA
                     TYLVDHIAEVPAMVIALRLDRLPEGASNAEIAGYHGSVAPGVWSFQLAARARGLGSAW
                     TTFHLFHEQEVAEMLGIPETVTQVALLPVGYYKGSGFTPATRRPARDITYFNAWKTTI
                     "
     gene            14677..16659
                     /gene="acs"
                     /locus_tag="VLR27_07140"
     CDS             14677..16659
                     /gene="acs"
                     /locus_tag="VLR27_07140"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HSP03259.1"
                     /translation="MSESQSGPAKTDGAAIEALGGETRTFPPPADFQAQALVSDRSLY
                     EEADADRLAFWSRQARELLTWRQDFDTVLEWELPHAKWFVGGTLNVTENCLDRHVQAG
                     NGDKVAYHWEGEPGDTRTFTYAELLDEVARFANVLKGLGVAKGDRVCLYMPMIPELPI
                     AMLACARIGAPHSVVFGGFSSNALADRINDAEAKVLITADAGYRRGEPSTLKPAADVA
                     LADTSSIDHVVVVDRCGTDPEMTEGRDHWWHDLMADASTDCPAEEMDSEDLLYLLYTS
                     GTTGMPKGIMHTTGGYLTQVAFTHKYVFDIHPDTDVYWCAADIGWVTGHSYIVYGPLT
                     NGVTSVMYEGVPNHPGNDRLWDIIERYGVTKFYTAPTAIRTFMKWGRQEPDGHDLSSL
                     RLLGTVGEPINPEAWIWYHEVIGGEGCPIVDTWWQTETGGIMISPLPGVTTTRPGTAT
                     HALPGISAELVDDEGNPVAEGGGYLTITEPWPSMLRGIWGDEQRYVDTYWSRFEGRYF
                     AGDGAKIDPDGYLWVLGRVDDVMNVSGHRLSTTEIESALVSHPSVAEAAVVGANDPTT
                     GQIPIAYVIVRGDVEVTDDLGEILRQHVAKEIGPIAKPKTVVLVPELPKTRSGKIMRR
                     LLRDVAEGRDLGDTTTLADAGVVDEIKTRAESGSGE"
     gene            complement(16666..17169)
                     /locus_tag="VLR27_07145"
     CDS             complement(16666..17169)
                     /locus_tag="VLR27_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03260.1"
                     /translation="MRLSGLAALAAGIVLAVPACGASGDAAWEPLRPGNGAATPVSPA
                     TSPPAPLCDAIPGDLVVTATGRSPVTSDGAGTQCSWRSAASTAEPDVVLEGSFIDTRT
                     FDVGRPGAAATTVHGIGDDAYLVEPGDGAPTTIYVRDGHRAFALWLTHPAAEGALTQL
                     ARQVLAG"
     gene            17245..17901
                     /locus_tag="VLR27_07150"
     CDS             17245..17901
                     /locus_tag="VLR27_07150"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HSP03261.1"
                     /translation="MRRWLALLVPLVFLVACGDDDDAANGTTTTVVDDATLEDDAAEP
                     GTDDDTGVEEASDDTAPETTVPGDAPAADGPGDDAGAGDGLGDAVLASDLEGSAPVPG
                     PGDPTGNGRFEAELVDGTLCVDMRITDLDASVTEAHLHSGRVGEDGPVLVDIGPPTST
                     DDGAGTWDDVCTGVDDDVIADLAAAPELAYVDVHSATFPDGAVRGQLSVISIFDRTLD
                     "
     gene            17903..18394
                     /locus_tag="VLR27_07155"
     CDS             17903..18394
                     /locus_tag="VLR27_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03262.1"
                     /translation="MAWHDPDNWAWRDGRVDPGETVVFDLDGVLSDATRRQHYLEWPR
                     RDWETFFEECGEDELIAEVARVLECLDEDLRVVLLTARPIRVQHQTLRWLERYDLRWD
                     LLVMRDWGDYMAAPSFKRLTVHELRDYGFDLRLAFEDDQRNVDMFHDEGVPCVYIHSG
                     YHT"
     gene            18391..20619
                     /locus_tag="VLR27_07160"
     CDS             18391..20619
                     /locus_tag="VLR27_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSP03263.1"
                     /translation="MREIQREQAHIDRAYEALDRLREGAAAAGPSALDDDEGAGGTFQ
                     ERFERERATADARRRLGELRWGDPPLVFGRIDLAEPVEGEDRFHVGRIGVFAEDQTQL
                     VVDWRAPIAEGFYRATRADSMGLRRRRAFHCRGRRLLAIDDVVLDPEAGEETAADGAL
                     VGEAALLASLEGPRTGRMSDVVATVQAEQDEVIRAPMAGLTIVQGAAGTGKTVVALHR
                     AAYLLYTFRDVLDRQGVLVLGPNGRFLDYVRDVLPSLGEHDVRLATVHQLYPGVGAVP
                     DDDVDVASLKADLRMVRVVRRALRMRQRRLRERASIPVGRFILKLDPPVVNHIVDTVR
                     GLEGTHNQRRRIVEDDLVAALDTELRRRNTASVDRMRDPGRDGLRHLLRRTPAFAELL
                     DRVWPLLRPEELLHDLFGSEAMLRHAARNGLLTPDEQRLLHRDRSASWRDVEWTHADV
                     PLLHEAHALLGPLPVPPPPSTDPTEDEQYLVDRVIEHVLLAMEQGMDPVMEADLRRSV
                     LDEIRVDTDTTIDAARRPDVLPVWGTVLVDEAQDLSPMAWRMVARSCPNQSMTIVGDL
                     AQGTAPWSPWSWDEVVTWIAPRHPARRHELHVNYRTPTEIMAWAAPLSAAPSRARAVR
                     SSGHEPRAVAVDAAQLVETAAQHALDLRDAAPDGRVAVVGSTCAAAELQQRVGDAVEV
                     HTPETVKGLEFDGVVVVEPSDLLGPGGTTAPLYIALTRATDRLVVVHARPLPDALAGA
                     VR"
     gene            complement(20623..21018)
                     /locus_tag="VLR27_07165"
     CDS             complement(20623..21018)
                     /locus_tag="VLR27_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSP03264.1"
                     /translation="MNRPPTVLYVEDEAPLRGLVQFWLEDAGYRVLLAADGAEGLDMA
                     RAEHPDLVITDAMMPNLSGDELVEIMRDDPDLCTIPIVMATAAASPLRVERMRERGCH
                     AVLGKPLDEQTFLAAAAAALAAAGGTPPG"
     gene            21156..22004
                     /locus_tag="VLR27_07170"
     CDS             21156..22004
                     /locus_tag="VLR27_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HSP03265.1"
                     /translation="MRNTAKTAILLAGLGGLLVVIGGGIGGGSGATIGLLIGLVFVGG
                     SYWFSDKLAIKAARAVPANEAEHPQYFATMRELSQLAGIPMPALYISPSPQPNAFATG
                     RNPEHAAVAVTEGLVRALEWDEIRGVLAHEISHVGNRDILISSVAAAIAMGITFVARM
                     AMWGAMFGGGRSDNDNNPIGMLAMALLAPIAAGLMQMALSRSREFQADETGARLIGTG
                     EPLARALQKLELAAHRVPMDIDPAHAQAFIVNPLTGRKVQFADLFRSHPSTEDRVARL
                     RGGSWA"
     gene            22086..23321
                     /locus_tag="VLR27_07175"
     CDS             22086..23321
                     /locus_tag="VLR27_07175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSP03266.1"
                     /translation="MGAHRRLDRILALAVALVVLAAGAPAYAQIIGDDPFGSPDDTAP
                     PSSEPEPEEPAPTTTRPPSEPSQPSEQPQSDPAPPPPGPAPSDAPAGGEAPPPDGGDS
                     TSPPPSSTARGIPPEAQAIMDSIQRSPANNSKALYDAVISQLVPLGLSEEEAIRIGFG
                     RFPIAGVANFVHDWYFPRWGPGFRFHMGTDVFAAHGTPVRAPVDGTVTSGSGSLGGLF
                     VKVFQPDGTYFYMAHLSGLVEGFQEGMAVKTGDIVGYVGNSGNARTTPPHVHLGIYTA
                     GRDATDPKPILDQMLADALAALPGVVEQVKAQRGDAPAPAPTATAEPTARVPQSLLAS
                     SLLRSLVDERAAGSLDTAVLLEAVGNPSSAGLAVAEAEASQLAASIDWDARAARTRTE
                     RELVERVGQMFRSALGPLG"
     gene            complement(23326..23817)
                     /locus_tag="VLR27_07180"
     CDS             complement(23326..23817)
                     /locus_tag="VLR27_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HSP03267.1"
                     /translation="MPSFDVVSQFDVQEVRNAVDQTAREASTRYDFKGTDSSIELSGE
                     ESITLRSGSEDRLHALRTVLEEKLVKRKVSLKVLDPGKVEEAAGGSARQTIALRAGIT
                     SDKARELNKFIKGLGVKGIQSQTQGEQLRVTGKSRDSLQEVIAALREGDFEVPLQFEN
                     FRD"
     gene            23885..23967
                     /locus_tag="VLR27_07185"
     tRNA            23885..23967
                     /locus_tag="VLR27_07185"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23919..23921,aa:Tyr,seq:gta)
     gene            complement(24034..24519)
                     /locus_tag="VLR27_07190"
     CDS             complement(24034..24519)
                     /locus_tag="VLR27_07190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP03268.1"
                     /translation="MLLRPATPADAAATAAIYNAEVTGSRVTFDLVTRTVEEQSAWLR
                     SRSGALEVVVAEVDGEVAGFASLSPYRDRPAYRTTVEDSIYVHADHRGSGVGRALLEE
                     IVGVARERGFHAVMARIVADHAASIRLHAAVGFEHVGVEREVGRKFGRWLDVTVMQLL
                     L"
     gene            complement(24539..25837)
                     /locus_tag="VLR27_07195"
     CDS             complement(24539..25837)
                     /locus_tag="VLR27_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03269.1"
                     /translation="MRLERLVIGSGEHTLSADFHPELTVIRGLSPSTREALAGEVIDA
                     LAGARPGVHLEVQAGGRSLTVFRPETGRHRVIDTDSVRDVTEDHLGADGEIDLFASAG
                     VDRALARRTIRFTSDDLVPEHESDAWIARLGAADQEALWDTAMRCRASERLLEQASAG
                     GGVSMDDAPIVREIEERHAALVAATDSYERVRLIALTIGTVGALGAVGMLNLEGSPAA
                     LPFLLIALFGLALGLRFRRSVDDAAKAERNVLRQAGADDYAAFHYERVSALLDTDHER
                     RAFMRAVGDHRRAMAAWAQVAGPAPLLFALEHEREIRAAAELHAGLGAHTSASVGAAG
                     DDVSAELAQAILARVRAVRALTPGDSLPLIVDDPFEGLDAAVKPLLLEMLAAHAGDPQ
                     LVVVTSDEDVVAWARDEARRGRMSVVEPTIDDGAIAAPSA"
     gene            25994..26066
                     /locus_tag="VLR27_07200"
     tRNA            25994..26066
                     /locus_tag="VLR27_07200"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26027..26029,aa:Thr,seq:ggt)
     gene            26102..26175
                     /locus_tag="VLR27_07205"
     tRNA            26102..26175
                     /locus_tag="VLR27_07205"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26136..26138,aa:Met,seq:cat)
     gene            26265..>27301
                     /gene="tuf"
                     /locus_tag="VLR27_07210"
     CDS             26265..>27301
                     /gene="tuf"
                     /locus_tag="VLR27_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HSP03270.1"
                     /translation="MAKEKFDRSKPHVNIGTMGHIDHGKTTLTAAISRVLSERVDGNN
                     ATAFDMIDKAPEERERGITINVAHIEYETDKRHYAHVDMPGHADYIKNMITGAAQVDG
                     AILVVAATDGPMPQTREHVLLARQVGVPAIVVALNKVDMVDDEELLELVEMEVRELLS
                     DQDFDGDNAPVVPVSALKALEGDSDAGDQVIELMNQVDEYIPEPERDLDKPFLMPIED
                     VFSIQGRGTVVTGKVEQGVIHVGDDIEIVGIRDTQKTTCTGVEMFRKLLDQGQAGDNI
                     GALLRGIDKDGVERGQVLCAPGSITPHTEFEATVYVLTKDEGGRHKPFFSNYRPQFFF
                     RTTDITGSIELP"
BASE COUNT         3789 a        10023 c         9510 g         3979 t
ORIGIN      
        1 agagagacgt accgacatgg atttcatcca ggactgggga ctcaccgcga tggtgttcgc
       61 gccgctcgcc ggcgtcgcca tcatgatgct gttccccaag gacgccgacg acacccacaa
      121 ggtgctggcc ctgctgacct ccctggtcac cgcagcgatc ggcatcgcag tgctcgccga
      181 cttcgactac gacgacacgg ggtcgctgca gttcctgcgt gacaacgagt ggatctcggt
      241 catcaactcg cggttcatcc tcggcatcga cggcctgtcc gtgccgctga tcgcgctgac
      301 cctgctcatc acgccgctgg tgatcgccta ctcgtggaac cacttccccg agccgcacaa
      361 ccccaaggcg ttcctgtccc tgatcctcgt gctgcacacc ggcatgctcg ggaccttcgt
      421 cgcccaggac ctgatcctct tcttcgtctt cttcgaggtc gtcctcctgc cgatgtactt
      481 catgatcggc gtgtggggcg ggccggagcg gcagtacgcc tcgatcaagt tcttcctcta
      541 cacgctgttc ggctcggcgc tgatgctgct gagcttcctc gcgctgttct tcctgaccgg
      601 cggggagacg ttctcgatcg tggagctggc cgagcgggtc cagggcatgg acatcgcccg
      661 caccacccag ctgatcgtgt tcggcggcat gttcatgggc ttcgccatca aggtgccgat
      721 gttcccgttc cacacctggc tgcccgacgc ccacacccag gcccccacgc agggctcggt
      781 gatcctggcc gccgtgctgc tgaagctcgg cacctacggc ttcatccgcg tcgcgctgcc
      841 gatcctgccc gaggccgccc agtcctgggc cccgtggatc ggtgggctcg ccgtcatcgg
      901 catcatctac ggcgccctcg ggtgcctggc ccagaccgac atgaagcgcc tgatcgcgtt
      961 ctcctcggtg gcccacatgg gctacacgat gctgggcatc tccaccctca ccgacttcgg
     1021 cctgaacgcc gccgtcttcg gcatggtcgc ccacggcctc atcaccggca tgctcttctt
     1081 catcgccggt tcggtgaagg agcgctacca caccctcgag atcaagcgcc tcggcggcct
     1141 gctggtctcg gccccccgcc tcggctggat cctgggcttc tgcacgatgg ccagcctcgg
     1201 actccccggc ctggccgggt tctggggcga gttccccgcc atcctcgccg cctaccagcc
     1261 cgccgagggc ctgccggtcg agctgttccg cggctacatg gtcgtggccg ccatcggcac
     1321 cgtgctcgcc gccggctacc tgctctggct gtaccagcgc accgccttcg gcaccgtcaa
     1381 ggacgagttc gtcgacgacc ccaacatcca cgacgtcaac ggtccggagt ggatcgcctg
     1441 gacgccgctg ctcgccctca tcctcctgct cggcgtctac ccgaacctgc tcttcaaggt
     1501 ctccgatggc tccatcgtga acgtggcgca gcagatcgcc gcagcgaccg ggaactgatc
     1561 cagtgctcgc cgtgctcgcc caggtcagcg acttcgtcgc cccccacatc gacttccacg
     1621 cgttcggtcc cgagctcgcg ctggtcggcg cgctcgtggc cgtcatggtc gtcgacctga
     1681 tcaagccgga tgggaacaac ccgttcctgt cctccatcgc cggactcggc gtgctcggcg
     1741 ccatgatccc cgtgatcacc ctggccaccg atggtgccga ccggttcctg ttcgacggcg
     1801 cctacgtggt cgacaacttc tcgctcgtgc tcaaggcgct gttcctcgtg tcgggctacc
     1861 tcgtcatcct gatgtcgacg aactacatcg ccgagggcga ctacgccgag ggggagtact
     1921 acctcctgct gctgtcctcg ctgctcggca tgatcctgat ggccagtgcg cgcgacctca
     1981 tcacggtgtt catcgccctc gagctgctct cgatcccggc ctacatcctc gccggctggc
     2041 gcaagcggga cctgatgggc aacgaggccg gcgtgaagta ctacctgatg ggcgtgttcg
     2101 cctcggcggt gctgctctac ggcatgtcgc tgctcttcgg cgtcgccggc gacacccgcc
     2161 tcaccgagat cggccaggcc atcgccggcg gtgcgggcga gaccccgctg atcaccctcg
     2221 ccgtcgtctt cgtcgtggtc gggttcgcct tcaaggtgtc ggcggtgccg ttccacacct
     2281 gggcgcccga cgtgtaccag ggcgccccca cgccgatcac ggcgttcctg gcggtggcgt
     2341 ccaaggcggc cggcttcgtg gccctgctgc aggtgatcta cctcgccttc ccggcgcgcg
     2401 acgacatcat ccagccgctg atgtgggtgc tggccgccgc caccatgacc atcggcaacc
     2461 tgatcgcgct ccgccagacc aacgtgatcc ggctgctggc gtactccggc gtcgcccagg
     2521 ccgggttcat cctcgccccg ctgtcggtgg actccgacct gtcgctgcag gccgtcatca
     2581 cctacctggt gatctatgcg gccatgaacc tcggcgcgtt cgccgccgtc atcggcatcg
     2641 cccgcaagac ccgttccggc gagatctcgt ccttcggcgg gctcttcgag tacgccccgg
     2701 gcctcacggt gatcctgtcg ctgttcctgt tcagcctggc cggcatcccg ccgctgggcg
     2761 gctggctggc caagttcgtg gtgttccggg ccctggccga tgccaacacc gcctcgggct
     2821 acgtcatggg cgccatcgtc gccgtgaact cggtgatcgc gctgtactac tacgcgaacg
     2881 tcgcccgtga gatgtggatg aagcccgtgc ccgacggtga ccgcagcccg gtcgtcgtgc
     2941 ccttctccat cagcgccgcc ctggccctca ccgtggtcgc caccgtcgcc ttcggcgtct
     3001 cgaacctggc cacccgcctc ggcgaccagg ccacgttcct cctggcgctc tgaggagcac
     3061 cccgtgaccg gcgagccggt ccacaccttc gtcgagcggg ccctgtacgg gtccgacggc
     3121 ttctacgagc agggcggccg cgccggccgc cgcggcgcgt ccttcgtcac cagccccgag
     3181 gtcggcccgc tcttcggtgc cgtgctggcc agtgcgctcg accgctggtg ggacgacgcc
     3241 ggccggcccg acgagtgggt cgtggtcgac gccggcgccg gccccggcac cctggcccgc
     3301 gccgtgctcg ccgcccagcc cgcctgcgcc gccgccctgc ggctcgtctg cgtggagcgc
     3361 acctccgccc agcgcgagct ccaccccggt ggcgtcacct cgaccgaccg gctcccggac
     3421 gtggccgacg tcatcgtcgc caacgagctg ctcgacaacc tggccttcga cctcctcgag
     3481 cggaccgacg ccggctgggt gccgatcgag gtgcgggccg acgggtcgac gcaacccgac
     3541 acggcgggac cgcgcatccc ggtggccggg gacgcctgcg cctgggtcac gaccgcccgc
     3601 cgccggctcc gaccccacgg ccggctggtg tgcttcgact acgccgacac cacggccgcg
     3661 ctcgcagcgc ggccctggac cgactgggtc cgggccttcg ccggccacga gcaggtcgcc
     3721 gacccgttcg cccagccagg cacccgcgac gtcaccgtcg tcgtgccctg ggaccagctg
     3781 cccacgcccg agcgcagcac cgaccaggcc acgtggctgc gcgagcacgg catcgacgac
     3841 ctcgtcgccg agggcatcgc ccactgggag gcccacgccg ccgcccccga cctcgaagcc
     3901 atgcggatgc gcagccgcgt gcgcgaggcc gaagccctca tcgatcccgc cggcctcggg
     3961 gggttctggg tgggggagtg gcgcccctga ggaactggca gcggctgcgg aacgacgtga
     4021 cggaagcccg ctgaccgctg ctcccggctc cggtaccgtc cggcccgtga ccccggtgat
     4081 ccgctgctcg tcgctcgtga agtccttcgg cgcgacccga gcgctcgacg gcctcgacct
     4141 cgaggtcgcc cccggtgagg tgcacggctt cctcggcccc aacggcgccg gcaagaccgt
     4201 caccatgcgc atcgtcctcg gcctcctccg tgccgatgcg ggcgaggtca ccctgctcgg
     4261 cggcgacccg tggcacgacg cagtggccct gcaccggcgc ctcgcatacg tgcccggcga
     4321 cacgaacctg tggcccaacc tcaccggcgg cgagatcatc gacgtgctcg gcggcctgcg
     4381 cggcgggttg gacgacggac gtcgcgctgc gctggtcgac cgcttcgacc tcgacccccg
     4441 gaagaaggcc cgcacctact ccaagggcaa ccgccagaag gtcgcgctcg tggccgccct
     4501 cgcctccgat gccgagctgc tggtgctcga cgagccgacg tccggcctcg atccgctgat
     4561 ggaggcggtg ttccaggact gcatcctcga cgcccgggcc gagggccgca ccgtcctgct
     4621 gtcgagccac atcctggccg aggtggagaa gctgtgcgac cgggtgacca tcatccggcg
     4681 cggtgccacg gtgcagaccg gcacgctcgc cgagctccgc cacctcacgc gcacgacgat
     4741 caccgccgag gtccgggagc agccgaccgc gctcgacgag ctggccggcg tgcacgacct
     4801 ggtggtcgac ggccaccggg catccttcga cgtcgacagc gatcacctcg acgaggcggt
     4861 cgaccggctg gccgcgctcg gggtcacgtc gctcaccagc cacccgccca cgctcgagga
     4921 gctgttcctg cgccactacg gcgacgagct cgccgacgtc ggggtggacg agcccgtcgc
     4981 acgagccgac ggtcatccct gatggtgacc ctgaccggac tcggacgcct cgtgcgcctg
     5041 gtcctgcggc gggatcgggt ccggctcccg gtgtgggtgc tcggcctcgc cggtgtcgtc
     5101 atcggctccg gcgcgtcgct cgtgccgctg tacccggagc aagccgacat ccagcagtac
     5161 gtcgacctgt tcggcgacaa cccggcgctc gtcgccttcg ccggcccggg ctacgggttc
     5221 ggcgacccca acctcggcgt gatcctggtg aacgagaccc agctctgggc ctgcatcggc
     5281 gtggccctga tgagcatctt cctcgtcaac cgccacaccc ggggcgagga ggaggccgag
     5341 cgcaccgagg tcctccgctc gagcgtcgtc ggtcgccacg ccccgactgc agcggccgtc
     5401 gtcgtggtcg ccgccgcgaa catcgcgctc gccgcactgt gcggcatcgc gttcgtggcg
     5461 ctcggctacg gcgtgacggg ctcgctcgcc tacgccgccg ggctggccgg ctgcgggctc
     5521 gtcttcaccg ggctgaccgc ggtcctcgcc cagctcgccg gcagcagccg ggccacgctc
     5581 gggctggcca ccgccgtgct cggcgccgcc ttcgtcctgc gggcggtcgg cgacatcacc
     5641 ggcaacgcgc tgcgctggct atccccgatc ggctgggccc agggcgtgcg ggcctacgcc
     5701 gacgagcagt ggtggccggt cgccctctgc ctcgccgtca cggtgctggc ggtggctggc
     5761 gccttctggt tggcgacccg ccgcgacctc ggatcgggga tcctgccgca gcgacccggt
     5821 ccggcgcggg ccggctggtg ggcgaccagc ccactcgggc tggcggtgcg gctccagcgg
     5881 ggcgcggtcg tggcgtggat ggccgggctc gtcctgatgg gcggcgtcta cgggtccatc
     5941 ggcgaggacg tcgagcagat gatcgaggag aacgagacct tcggcgacgt cctcgcccag
     6001 ctcgacggcg ccgacgtcac cgacgcgttc ttcgccacgg ccgtgtccat gcaggcgctg
     6061 ctggccgccg gcttcgccat ctccgccgcg ctgcggatcc ggtccgagga ggccgccggg
     6121 cgggtcgagc ccatcctcgc cggtcccgtc gggcggtggc gctgggcgtg gagccacctg
     6181 ctggtcgccg cggtcggcac cgtgctggtg gtcgccgccg gtggcgtggg cgtcggggcc
     6241 tcctacgccg tggtgtccgg tgacgccggc caggtcctgc gcctcaccgg cgccgccctc
     6301 gccaccgtcc ccgccgtgct ggtgctggcc ggggtggccg tcgccctgtt cggggcctcg
     6361 ctgcgcctcg ccctcgtggc ctgggccggc ctcggcgtcg tcgtcctcgt cgagctgttc
     6421 ggtgagctgc tccggctgcc gtcgtggtcc cggatgatct cgccgctgca ccacgtgccg
     6481 ggggtccccg ccgaggacct gcgcgccgtg ccgctgctcg tcctcaccgc catcgccgtg
     6541 gccctcaccg ccgtcggcat ggcccggttc cggtcgaggg acctgcaggt gggctagcga
     6601 ccgccgtcgg gatcgttcac gtcgtcgtct gcgacggcag gaggtcgggt cgtggccgga
     6661 gatccctggg cgatcacccg ggcggcgcga gctgcagcca ctcgtagaag gcagcccggt
     6721 cgccatcgaa ggagtagacg acctcgtcga cctgcgggaa ctggagcacg gtgttgtcga
     6781 gctgggcgag caacccggcg ctgccgcacg acgacgaggc gttcgggatg atccccgaga
     6841 agtcggcgaa gtcgaccgcc gccacgccgt cctcgacggt caggccgttg agcatcccgg
     6901 cggtctccgc ggagaaccag ctgcgcaggt cctgtgcctg ctcctcggcg gtgggaccgg
     6961 ccagcagctg cgtgagggcg tcgtccacgt ccccctcgag cgcctcgcgc tcggacggga
     7021 tcaccacgct gcagtcgttg ccggtctccg ccggcagcag gtacacgtcg aaggtggtcg
     7081 gcgcttcggt cgagtcgggc tcgctggtgg tggtcgcctc ggtggtggtc gtgtcggtgg
     7141 tggccgtctc gtcgtcgtct ccgcacgcag cgacgaggac gcccagcatc gcgatcgtca
     7201 tcaggagcac gcgggtggag gtgcgcatga ggacagcatc ctgtgacacc gagtgagcgg
     7261 acagggcaga aggtcacccc cgtcgtcggg tcgctgatcc cgtgtcggcg cgtgacgttc
     7321 ggccctggtg ccccaggcgc ggccggcgaa gactggtggg catccagctc gatggaggtg
     7381 aggggtgagc gttcggtggt cagtcgtcgt cagcgtggtg ttcttggtgc tggccccggg
     7441 ctgcggcgac gacgatgacg gcgagcccgg catcgcgaac ccggcctcag agttctgcga
     7501 ggagcagggc ggacgtgtcg agatggaccg ggatggcgcc ggcaacgagc gggggatgtg
     7561 cgtgctcccc gacggcaccc gagtcgacga gtgggagtac taccgggagc agggcggcga
     7621 gtagcggggc tcgccgtcag tcgtcgatcg ggtcggagat ccggaggcgg ttgccgtcgg
     7681 ggtcctccac cttgagctcc cggccccacg gcacctcggt gagctcctgg cccaggcgtc
     7741 gggcgaggtc gtcgagctgg ctgtggcgga tgtagacgag cgagtcgggg ctggcgtcgt
     7801 cgtcgtgctc ggagaggtag atgtgcccgc gcccgcgggc gatggacgcg aaccccggct
     7861 ggtcgggctc gatgcggtgc tcccactcct tgtggaaccc gagctgggcg taccactcga
     7921 ccgactggtc gatggacgcg actcgcagga tcgggatcac ctcttgcgac acggcggcga
     7981 cgctactggc cgggatcgac acccggcctg ccaccggccc cctacggtgg ccccatgcgc
     8041 accgccgtca ccgaacgcct cggcatcgat ttcccgatcc tggccttcag ccactgccgc
     8101 gacgtcgtcg ccgccgtcac caactccggg ggcatgggcg tcctcggtgc cgtggcccac
     8161 agccccaagc agctcgagat cgacctcgac tggatcgcgg ccgagaccaa gggcaagccc
     8221 ttcggcgtcg acctgctgat cccgcagaag ttcgtgggcg ccgacgacgg cggcctcgag
     8281 gtcggcagcc tgcgcgacct catccccgcc gagcaccgcg agtggctcga cggcctgctc
     8341 gcccagaacg gcatcccccc gctgcccgac gacgacgccg agtccgtgcg cagctacggc
     8401 ggcatgcggg tcgatccgaa gtcgatggcg cccctggtcg acatctcctt cgccaagggg
     8461 gtctccctca tggcatccgc gctcggcccg cccccgccct ggctcatcga acgggccaag
     8521 gccgagggcg aggtcccggt ggccgcgctc gccggttccc tcgagcacgc catccgccac
     8581 gaccaggccg gcgccgacct gatcatcgct cagggcacgg aggccggtgg gcacaccggt
     8641 gaggtcgcca ccatggtcct ggtccccgag gtcgtcgacg ccgtgcaggc acccgtgctc
     8701 gccgccggtg gcatcgggcg gggccgccag atcgccgcct ccctcgccct cggcgccgag
     8761 ggggtgtggt gcgggtcggt gtggctcacc acgcaggagg ccgagacctc cgacgtcatc
     8821 aaggacaagt tcctggccgc accgtcatcg ggcaccgtcc gctctcgctc gctcaccggc
     8881 aagccggccc gcatgatccg caccgactgg accgacgcct gggagtccga gccctcgccg
     8941 ggcccgctgg gcatgccgct gcagtcgctg ctggtggccg aggcccagag ccgcatcgcc
     9001 cgggtcgcgg ccaaggacga aggcgctcgc cgcctctcca cctacttcgt cggccagatc
     9061 gtgggctcca tgaacacggt ccagcccgcc gcccgcgtcg tgctggacat ggtcgaggag
     9121 ttcatcgacg ccgtcgagcg cctcgacggt ctgctccagg actgagcggg gcacagaggg
     9181 gcgatgccga gcgagctgct gcgcaccgac caggtcacca tcggcggggt gccgacccga
     9241 gccgtccggg ccgacggcga tcccgaccgg gtgcggttcc tcttcctgca cggcttctcc
     9301 gacagcgccg actgctggca gcgggtccag aagcgcctcg ccgccgccgg tcacggctcg
     9361 ctcgccgtcg accagccatc ccacggccac gccgaccccc tccacccgga cctgcccgtc
     9421 atcgagcagt tccttgcctt cgccgccgcc gcagcgcgag ccaccgacga cggtcgcccg
     9481 gtggtcgtgg tgggcaactc cctcggcggt gcccacgccc tgctgctcgg ccagcaccac
     9541 cccgagctcg tgcacggcgt ggccgccatc tccccggcgg ccttcgacca cccgggctgg
     9601 ttccgcatcc tggactcccg cttcaacgtg tcgccggagg gcgtcgtccc caccgtggtg
     9661 cgacgtgccc tggccgggcc ggccatgcgg gccgtcggct tcgggatgcc ccaccgggtc
     9721 ccggccggct tcgtgcgcac ctgggcccag agctggcacg accccacccg gcggcgtgcc
     9781 ctgcacgacc tcgccacccg cgtcccggcg gagtacttcc acgccgaccc ggtggacctg
     9841 tgcgccgtcg acgtgcccgt cctggcgctc tggggcacgc gcgaccgcct cacgctcatc
     9901 agcagccgcc agcgcttcga gcaggactgc accgacctcg agttcgtggc cctgcccggc
     9961 gtcggccaca tggcccaggt cgaggtcccg ggacgcacca ccaagcacct gctgtccttc
    10021 gccgcacggc tgaccgacgg cgccacgccg gccggcgcca ccgaggccac gctggtggcc
    10081 agcgcctgac cctcgccgtc tggctccggc cggaccgcgt ctgctggact gacaacgacc
    10141 gttgccagcc cgcacgtcga ggggaagtcg atgaccgcca ccaccactgc gcccggtgcg
    10201 ctgagcccgg acgaggccgc cgcgttccgc cagcgctgca tcgagttcct cgacgagcac
    10261 gccaccggca tccacctcga cggcgacccc gacccccggg ccgagaagcg cctccgggcc
    10321 gcgcaggcct tccagcaggc gctcgccgac gccggcctcg ccggcatcac ctaccccgcc
    10381 gagtacgggg gcgccgggct caccaaggag cacgacaaga tctggcggga gtgctacgcc
    10441 cagtacccct tcatgaccag cgagctcacg atcagccacg gcatgtgctt gccgatgctg
    10501 gccgagtacg gcaccgagga ccagaagcag cgctacctgg ccgacaacat cgcagcccgc
    10561 acggtctggt gccagatgtt ctccgagccc ggtgccgggt ccgacgtcgc cagcctgcag
    10621 agccgggccg tcctcgacgg tgaggagtgg atcctcaacg gccagaaggt gtggaccacc
    10681 ctcgcccacc tctgtgactt cggcatcgtc atcgcccgca ccgatcccga ccagcccaag
    10741 cacgccggca tctcgatgtt catcgtcgac atgcgcgcgc cgggcgtcga gatccgtccc
    10801 atccaccagg tcgacggggg cctgcacttc aacgagatct tcttcaccga cgtccgcatc
    10861 ccggcggaga acctggtcgg cgacctgaac aacggctggc gcctggccac cgcgatgctc
    10921 atgtacgagc gcgtggccat cggcggcggg ggcaccgacg gcgtcaccca cgccaacgcc
    10981 gatgcgctca tcctcgaggc ggagaagcga ggcgtgctcg gtgaccccgc actgcgcaac
    11041 gacctcatgg acctgtacat ccaggagtcg gtgaagtcga tgaacagcct gcgcacccgg
    11101 gccgagctga aggcgggcaa gacccccggt cccgccggct cgctcggcaa gctgcagggc
    11161 acgctcgtca gctacctggc gcgggatctg tcgatgcgcg tcgtgggggc cgacagcatc
    11221 gcctggggcg aggagggtgg ccaccggtgg tcgacccagg ccatccggtc catgggcagc
    11281 ggcatcgccg gtggcaccaa cgagatccag aagaacatca tcggcgaccg cgtcctcggc
    11341 ctgccccgcg agccgagcgt cgacaaggac gtgccgttca aggacctccg ggtggggacc
    11401 cagcgcgact gatcactcca ggccggtgcc cttccggaac tgctcggtgg tgttgatgtg
    11461 cgggaagggc tcgtcgggga cgggcacgcg gagggcctcg cagatgggct cccacccgtc
    11521 gccggtctgc cagatgagca gccggtcggc tggggcgctg gcgcgcacgt cggcgttcca
    11581 ggcctcgacc gcggccatga gggcatcccg gtcgaggaag tccggggtga agtcctcgaa
    11641 catcgtgacg gccatgtccc accacgcgtc ggtgccgggt gccggccact cgtcccgggg
    11701 cttcttgaac gtctcgagga tggtctggtc ggcggagcgg aaccactcgt cggccgggcg
    11761 ctccgagtgc aggatcagcg cgtccgggta ggcctcggcc agctccttcc agcacgcggc
    11821 cgacggccag tccaccgtgg cggcgaagcc gccgaagagg tcgtcccagt cggtgggcgc
    11881 accacggatc gccgcggtcc agcgggccgg gtgctcgggc cgggggaagc actccatcat
    11941 gtggtagcag ggctcgccga gcagcagctc gagcgcgtcc ttgagcgacc tcgtcccggt
    12001 ccggccgaga ccgcttccca ccacccgcag cgccatggcg cccagtgtgg cttagagcgt
    12061 cggtgcctgg ttggcgaacg cgagctcccg gtcctgctcg cgctggtcgt cgtcgagggg
    12121 acggctgccc cgcagctcgt aggtgccccg gtactcgggc agcacctcgc cgtgctcgtc
    12181 gagcatccag cgcatcgcca tccggcgggg cacgcgcacc acgtcgacct cgacgtaggt
    12241 gccgaacacg tggtcccaga acggggtcgt gacgccgtgg ttcttcaggg gcgcgtggaa
    12301 gtggtggatg aagtggtgct tgcgcaccat gtgctcgtag cggttggcgt acgggcgccg
    12361 gtgcgcccgc cagtggacga ggtcgtagaa gccgtaggcc accaggtagc cgatgccgac
    12421 gccccagccg aggtcggcgg tgccggccag gcggccgagg aacgggatga ggccgaggga
    12481 cacgagcagc acgccggtcc aggacaggta ccagctctcg agcacggtgt cctcggagcc
    12541 gtggtggcgg aggtgctcgc gtgagccgta gttcttgccg cgggcctcgt ggaacgcgaa
    12601 gcggtgcagc acgtactcca tgaagctcca cagcacgatg ccggccggca ggccgagggc
    12661 gaagaggagg ggggagtcgg gggagggcat cagggatcgg tcccttcggg gatcgggcgg
    12721 gcgaggggcc cggtggcttc gaggtcgtcg acgatctcgt gggcggcggt gagcggtccg
    12781 ggtgccgcgc cccagccgtg caggagcgcg tcgaggacgt ggcgaccggt ggcggtgggg
    12841 tcgaggagct cgtcgtccca gcgctgcacc acccgcagct ggacgcaccc ggtgagggcg
    12901 gcggcgaggg cgatggtgcg acggtcggcg ggctggctct cgtcgagcgc gccggtgcgg
    12961 gcggcggcgg ccagacggga ctcggccagg ccgagcaggc gcatcgccgc cgggacgacg
    13021 cggccggcct cctccggcgg gacggactcg gtgacgtcgg agagcagcag gagcagcagc
    13081 tgcatctcct cggggtaggc ctccgccgcg gtcagccaga accggccgaa cgcgacgacc
    13141 ggggccaggt gtgctgcgtc tgcctccacc ccgtcgatcg tccggtcgac gtcggcgccg
    13201 agcagtgcgg ccgacgtggc cagccgctcg atcgcctccc gctgtacctc ggccaccagg
    13261 gcgcccttgg acgggaagta ggtgtagacg ctgcccaccg cggcgccgac ctcctgggcc
    13321 aggcgctgca tggtgagggc gtccagcccc tcggcgaaca cgagggacag cgcggcccgc
    13381 aggtacgagc ggtggcgagc cagccggttg cgctcgcgcg cccccaccgg tgccaccggc
    13441 acggcatggg gatcggggac ggcagagagc tgaaccacgt tcagagtctg aacgacgttc
    13501 agacttgctg tcaacccccg acgggagcag atcgggcgcg ggtaccgtct ggcgccgtgg
    13561 acttcgatga cgtgatccgc atcgggtcgg agctgcccga ggtggaggtg tcgacgtcgt
    13621 acggcacgcc ggcgctgaag gtgcggggca aggcgttctg ccggttgtgg ggcgacgacg
    13681 agcgggagcg caagggcccc gaggtcgacg gcgacgtcct ggtcgtgttc gtggacgtcg
    13741 ccgagaagcg ggcctggatc cagacgtccg acggcgcctt gttcgaccag gcccactacc
    13801 acgggcacgg cgccatgctg gtgaagctgg acgaggtcga cgaggacctg ctgctcgacg
    13861 tgctcgagca gagctaccgg atcaaggcgc cccgcacgct gctgcgccgc ctcgacgact
    13921 gaggactgat cagatcgtgg tcttccaggc gttgaagtag gtgatgtcgc gggcggggcg
    13981 tcgggtggcc ggggtgaagc cgctgccctt gtagtagccc accggcagca gcgcgacctg
    14041 cgtgacggtc tcggggatgc cgagcatctc ggccacctcc tgctcgtgga agaggtggaa
    14101 ggtggtccac gccgacccga gcccccgggc ccgggccgcg agctggaagc tccacacgcc
    14161 cggggccacc gagccgtggt agccggcgat ctcggcgttg ctggccccct cgggcagccg
    14221 gtcgaggcgc agcgcgatga ccatggccgg cacctcggcg atgtggtcga ccaggtaggt
    14281 ggccgagtcc aggacccgct gctggccctc gtcgacctcg ccggccgcgt tggcggccag
    14341 gtacggccgg ccgacctgct ggtacagcgg cgcgatctgg gccttgatgt cggggtcgtc
    14401 gacgacgagc caccggttgc gctcgaggtt gccgcccatc ggggcgtgct gggcgatgtc
    14461 gatgcactcg agcagcagct cgcggggcac gggccgctcc aggtccatcc gcttgcggac
    14521 ctgcttggtg gtggtgagga ggcggtcgac ctggtcgagg tcgaaggtgt cagcggcaga
    14581 ggtcatgcct gcagcctggc ccaccgcacg cccggaccgc tcgcactatg gtcgccgcag
    14641 tcgccgacga aacgcactcc agggggatcc tgcgccatgt ccgagagcca gtccgggcca
    14701 gcaaagaccg acggggccgc catcgaggca ctgggaggcg agacccgcac cttcccgccg
    14761 cccgccgact tccaggccca ggccctcgtc agcgaccgct ccctgtacga ggaggccgac
    14821 gccgaccgcc tggccttctg gtcccgccag gcccgggagc tgctcacgtg gcgccaggac
    14881 ttcgacaccg tgctcgagtg ggagctgccg cacgccaagt ggttcgtcgg cggcacgctc
    14941 aacgtgacgg agaactgcct cgaccgccac gtccaggccg gcaacgggga caaggtggcg
    15001 taccactggg agggtgagcc gggggacacc cgcaccttca cctacgccga gctcctcgac
    15061 gaggtggccc ggttcgccaa cgtgctcaag ggcctcggcg tggccaaggg cgaccgggtc
    15121 tgcctctaca tgccgatgat ccccgagctg cccatcgcca tgctggcctg cgcccgcatc
    15181 ggcgccccgc actcggtggt gttcggcggg ttctcctcca acgccctcgc cgatcgcatc
    15241 aacgacgccg aggccaaggt cctcatcacc gccgacgccg gctaccgccg aggcgagccg
    15301 tccacgctga agccggcggc cgacgtcgcc ctcgccgaca cctcgagcat cgaccacgtc
    15361 gtggtggtcg accgctgcgg caccgacccg gagatgaccg aggggcgcga ccactggtgg
    15421 cacgacctca tggccgatgc ctccaccgac tgccccgccg aggagatgga cagcgaggac
    15481 ctgctgtacc tgctctacac ctcgggcacc accggcatgc ccaagggcat catgcacacc
    15541 accgggggct acctcaccca ggtcgccttc acccacaagt acgtcttcga catccacccg
    15601 gacaccgacg tgtactggtg cgccgccgac atcggctggg tcaccggcca cagctacatc
    15661 gtctacggcc cgctcaccaa cggggtcacc agcgtcatgt acgagggggt gcccaaccac
    15721 ccgggcaacg accgcctctg ggacatcatc gagcgctacg gggtcacgaa gttctacacc
    15781 gcccccaccg ccatccggac cttcatgaag tggggccgcc aggagcccga cggccacgac
    15841 ctgtcgtcgc tgcggctgct cggcaccgtc ggtgagccca tcaaccccga ggcctggatc
    15901 tggtaccacg aggtcatcgg cggcgagggc tgccccatcg tcgacacctg gtggcagacc
    15961 gagaccggcg gcatcatgat cagcccgctg cccggtgtca ccaccacccg accgggcacc
    16021 gccacccacg ccctgcccgg catctcggcc gagctggtcg acgacgaggg caaccccgtg
    16081 gccgagggcg gtggctacct gaccatcacc gagccgtggc cgtcgatgct gcggggcatc
    16141 tggggcgacg agcagcgcta cgtcgacacc tactggtccc gcttcgaggg ccgctacttc
    16201 gccggggacg gggccaagat cgaccccgac gggtacctgt gggtgctcgg ccgggtcgac
    16261 gacgtgatga acgtctcggg ccaccgcctg tccaccacgg agatcgagtc cgctctggtc
    16321 tcccacccct cggtggccga agccgcggtg gtgggcgcca acgaccccac cacgggccag
    16381 atcccgatcg cctacgtgat cgtgcgcggc gacgtcgagg tcaccgacga cctcggcgag
    16441 atcctgcgcc agcacgtcgc gaaggagatc ggcccgatcg ccaagcccaa gaccgtggtg
    16501 ctcgtgcccg agctgcccaa gacccgctcc ggcaagatca tgcgccggct gctgcgcgac
    16561 gtggccgagg gccgggacct gggcgacacc accacgctcg ctgacgcggg cgtcgtggac
    16621 gagatcaaga cccgcgccga gagcggcagc ggcgagtagc ccagctcagc cggcgagcac
    16681 ctgccgggcc agctgcgtca gcgcgccctc ggccgccggg tgggtgagcc acagcgcgaa
    16741 tgcccggtgc ccgtcgcgca cgtagatcgt ggtcggtgca ccgtcgccgg gctccaccag
    16801 gtacgcgtcg tcgccgatgc cgtggacggt ggtcgcggcc gcacccggcc ggccgacgtc
    16861 gaaggtccgg gtgtcgatga agctgccctc gaggacgacg tcgggctccg ccgtcgacgc
    16921 cgccgaccgc caggagcact gcgtgccggc accgtcggag gtgacggggg agcgccccgt
    16981 cgccgtcacg acgaggtcgc cggggatggc gtcgcagagc ggtgccggcg gcgaggtggc
    17041 gggcgagacc ggggtggccg cgccgttgcc cggcctgagc ggctcccacg ccgcgtcgcc
    17101 ggaggcaccg caggccggga cggccagcac gatgccggcc gcgagcgcgg ccaggccgga
    17161 gagacgcatg gactcctccg tacccaggcg gtgccgggtc agccctgggt cgggtgtccc
    17221 gggaccgccg gtagcctccc gcggatgcgc cgctggctgg ccttgctcgt tccgctcgtg
    17281 ttcctcgtcg cgtgcggcga tgacgacgac gccgcgaacg gcacgaccac gaccgtggtg
    17341 gacgacgcca cgctcgagga cgacgctgcc gaacccggca ccgacgacga caccggcgtc
    17401 gaggaggcat cggacgacac cgccccggag accacggtgc cgggcgatgc gccggccgcc
    17461 gatggcccgg gagacgacgc cggcgcggga gacggcctcg gcgatgcggt cctggccagc
    17521 gacctcgagg gcagcgcccc ggtgcccggt cccggcgacc ccaccggcaa cggccgcttc
    17581 gaggccgagc tcgtcgacgg cacgctgtgc gtggacatgc ggatcaccga cctcgacgcc
    17641 tccgtcaccg aggcccacct gcacagcggc cgtgtcgggg aggacggccc ggtgctggtc
    17701 gacatcggtc cgcccacctc caccgacgac ggagccggca cctgggacga tgtctgcacc
    17761 ggtgtggacg acgacgtcat cgctgacctc gcagccgcgc ccgagctggc ctacgtcgac
    17821 gtccactcgg ccacgttccc cgacggcgcc gtccgggggc agctcagcgt catctcgatc
    17881 ttcgaccgga cgctggactg acgtggcctg gcacgacccc gacaactggg cctggcgcga
    17941 cggccgggtc gaccccggcg agaccgtggt cttcgacctc gacggtgtcc tctccgacgc
    18001 cacccgacgc cagcactacc tcgagtggcc ccgccgcgac tgggagacgt tcttcgagga
    18061 gtgcggcgag gacgagctca tcgctgaggt cgcccgggtg ctcgagtgcc tcgacgagga
    18121 cctgcgcgtc gtgctcctca ccgcccgacc catccgggtg cagcaccaga ccctccgctg
    18181 gctcgagcgc tacgacctgc ggtgggacct gctcgtgatg cgcgactggg gcgactacat
    18241 ggccgccccg agcttcaagc gcctcaccgt gcacgagctg cgcgactacg ggttcgacct
    18301 gcgcctggcg ttcgaggacg accagcgcaa cgtcgacatg ttccacgacg agggcgtgcc
    18361 ctgcgtctac atccactccg gctaccacac gtgagggaga tccagcgcga gcaggcccac
    18421 atcgaccggg cctacgaggc gctcgaccgc ctgcgcgagg gcgcggccgc ggccggcccg
    18481 tccgcgctcg acgacgacga gggcgccggc ggcacgttcc aggagcgctt cgagcgcgag
    18541 cgggccaccg ccgatgcccg acgtcgcctc ggtgagctgc ggtgggggga tccaccgctg
    18601 gtgttcggcc gcatcgacct ggccgagccg gtcgagggcg aggaccggtt ccacgtcggg
    18661 cgcatcggcg tgttcgccga ggaccagacc cagctcgtgg tcgactggcg ggcgccgatc
    18721 gccgagggct tctaccgcgc cactcgggcc gattcgatgg gcctgcgccg ccgccgggcg
    18781 ttccactgcc gcgggcgacg gctgctcgcc atcgacgacg tcgtcctcga tccggaggca
    18841 ggcgaggaga ccgccgcgga cggcgccctg gtcggcgagg ccgccctgct cgcgtcgctc
    18901 gagggcccgc gcaccgggcg catgtccgac gtggtggcca cggtccaggc cgagcaggac
    18961 gaggtcatcc gcgcgccgat ggccgggctc accatcgtcc agggggcggc gggcaccggg
    19021 aagaccgtgg tcgccctcca ccgggccgcc tacctgctct acaccttccg cgacgtgctc
    19081 gaccgccagg gcgtcctggt cctcggaccc aacggccgct tcctggacta cgtgcgcgac
    19141 gtgctgccct cgctcggcga gcacgacgtg cgcctggcca ccgtgcacca gctgtacccc
    19201 ggggtggggg cggtgcccga cgatgacgtc gacgtggcgt cgctcaaggc cgacctgcgc
    19261 atggtgcgcg tcgtccggcg agcgctgcgc atgcgccagc gccgcctgcg cgagcgggcg
    19321 agcatcccgg tcggccgctt catcctcaag ctggacccgc cggtggtgaa ccacatcgtc
    19381 gacaccgtcc gcggcctcga gggcacccac aaccagcgcc gacgcatcgt cgaggacgac
    19441 ctggtggcgg cgctcgacac cgagctccgc cggcggaaca ccgcgtcggt cgatcggatg
    19501 cgcgacccgg gccgggacgg gctccgccac ctcctgcgac gcacgcccgc cttcgcggag
    19561 ctgctcgacc gggtctggcc gctgctgcga cccgaggagc tgctgcacga cctcttcggc
    19621 tccgaggcga tgctgcgtca cgccgcacgc aacgggctgc tgaccccgga cgagcagcgc
    19681 ctgctgcacc gggaccgctc ggcgtcctgg cgcgacgtgg agtggaccca cgccgacgtc
    19741 ccgctcctgc acgaggccca cgccctgctc ggcccgctgc ccgtcccgcc gccgccgtcc
    19801 accgatccga ccgaggacga gcagtacctg gtcgaccgcg tcatcgagca cgtgctcctc
    19861 gccatggagc agggcatgga cccggtgatg gaggccgacc tgcgccgcag cgtcctcgac
    19921 gagatccggg tcgacaccga caccacgatc gacgcggccc gccggcccga cgtgctgccg
    19981 gtgtggggca cggtcctggt cgacgaggcc caggacctgt caccgatggc gtggcggatg
    20041 gtcgcccggt cctgtcccaa ccaatcgatg accatcgtcg gcgacctcgc ccagggcacg
    20101 gccccgtggt cgccatggtc gtgggacgag gtcgtcacct ggatcgcgcc ccgccacccg
    20161 gctcgccgcc acgagctgca cgtgaactac cgcaccccca ccgagatcat ggcgtgggcc
    20221 gcgccgctct cggctgcgcc gagccgggcc cgggcggtgc gctccagcgg gcacgagccc
    20281 cgggccgtcg ccgtcgacgc agcgcagctg gtggagaccg ccgcccagca cgccctcgac
    20341 ctgcgcgacg cggcgcccga cggccgcgtc gccgtggtgg ggtccacgtg cgcggccgcc
    20401 gagctgcagc agcgcgtggg cgacgcggtc gaggtccaca cccccgagac ggtgaagggc
    20461 ctggagttcg acggcgtcgt cgtggtggag ccgagcgacc tgctcggccc cggtgggacc
    20521 accgcacccc tgtacatcgc gctgacccgg gcgaccgacc ggctcgtggt cgtccacgcc
    20581 cggcccctcc ccgacgcgct cgccggcgcg gtgcgctgac cctcagccgg gtggcgtgcc
    20641 gccggcggcc gccagcgctg ccgccgcagc cgccaggaag gtctgctcgt cgagcggctt
    20701 gccgagcacg gcgtggcacc cacgctcgcg catccgctcg acccgtagcg ggctggccgc
    20761 cgcggtggcc atgacgatgg ggatggtgca caggtcgggg tcgtctcgca tgatctcgac
    20821 cagctcgtcc ccggacaggt tcggcatcat cgcgtcggtg atgaccaggt cggggtgctc
    20881 ggcgcgcgcc atgtcgaggc cctcggcgcc gtcggccgcc agcagcaccc ggtagccggc
    20941 gtcctccagc cagaactgga ccaggccgcg cagcggggcc tcgtcctcga cgtagaggac
    21001 cgtggggggt cggttcacgg gatcgtcggc atggatcggg gggttcgggg cacgccgccg
    21061 cctccgacgg gaaccgctgc ggggcacgat acgttctccc tggtggacgg tgcccgaccc
    21121 gggcacccgg gcatgcagca cgactgcgaa ggaccatgcg caacacggca aagaccgcca
    21181 tcctcctcgc cggcctcggt ggcctcctcg tcgtcatcgg cgggggcatc gggggcggca
    21241 gcggcgccac catcggcttg ctcatcggcc tggtgttcgt cgggggctcc tactggttct
    21301 ccgacaagct cgccatcaag gcggcccggg ccgtgccggc gaacgaggcc gagcaccccc
    21361 agtacttcgc gacgatgcgc gagctcagcc agctcgccgg catccccatg ccggcgctgt
    21421 acatctcgcc cagcccgcag ccgaacgcgt tcgccaccgg tcgcaacccc gagcacgcgg
    21481 ccgtcgccgt caccgaaggc ctggtccgcg ccctcgagtg ggacgagatc cggggcgtgc
    21541 tggcccacga gatctcccat gtgggcaacc gcgacatcct gatctcctcg gtcgccgccg
    21601 ccatcgccat gggcatcacc ttcgtggccc gcatggcgat gtggggcgcg atgttcggcg
    21661 gcgggcgcag cgacaacgac aacaacccca tcgggatgct ggcgatggcc ctgctggccc
    21721 ccatcgccgc cggcctcatg cagatggcgc tgagccgcag ccgcgagttc caggccgacg
    21781 agaccggtgc ccgcctgatc ggcaccggcg agcccctcgc ccgcgccctg cagaagctgg
    21841 agctggccgc gcaccgggtg ccgatggaca tcgatcccgc ccacgcccag gccttcatcg
    21901 tgaacccgct caccggtcgc aaggtgcagt tcgccgacct gttccgcagc cacccgagca
    21961 ccgaggaccg cgtggcccgc ctccgaggcg gcagctgggc ctgacccgcc ggggtcgccc
    22021 gctgacccgg acccgacgag gcccatcggc agggaacacg gcgctccgtg gcgaatcggt
    22081 cacggatggg tgcacaccga cgactcgacc ggatcctggc gctggcagtc gcgctggtgg
    22141 ttctcgccgc cggcgcaccg gcctacgccc agatcatcgg cgacgacccc ttcggctcgc
    22201 ccgacgacac ggcgccgccc tcgtcggagc cggagcccga ggagccggcg ccgaccacca
    22261 cccggccgcc gagcgagccc agccagccga gcgagcagcc ccagtccgac ccggcaccgc
    22321 cgccgcccgg tccggcgccg agcgacgccc ccgccggcgg cgaggctccg ccgccggacg
    22381 gcggtgacag cacgtccccg ccgccctcgt ccaccgcccg cgggatcccg ccggaggcgc
    22441 aggcgatcat ggacagcatc cagcgcagcc cggcgaacaa ctccaaggcc ctctacgacg
    22501 cagtgatcag ccagctggtg cccctcgggc tcagtgagga ggaggccatc cggatcgggt
    22561 tcggccggtt cccgatcgcc ggcgtcgcca acttcgtcca cgactggtac ttcccccggt
    22621 gggggcccgg cttccggttc cacatgggca ccgacgtctt cgccgcccac ggcacgccgg
    22681 tgcgcgcccc cgtcgacggg acggtcacgt ccggcagcgg ctccctcggc ggcctcttcg
    22741 tcaaggtctt ccagcccgac ggcacctact tctacatggc gcacctgtcc ggcctcgtcg
    22801 aggggttcca ggaggggatg gccgtcaaga ccggcgacat cgtcggctac gtgggcaact
    22861 cgggcaacgc ccgcaccacg ccgccccacg tgcacctcgg catctacacc gccggccgcg
    22921 acgccaccga ccccaagccc atcctcgacc agatgctggc cgatgcgctg gccgccctcc
    22981 ccggcgtggt cgagcaggtc aaggcccagc ggggcgacgc ccccgccccg gcgcccacgg
    23041 ccaccgccga gcccacggcc cgggtgcccc agtccctgct ggcctcctcg ctgctgcggt
    23101 cgctcgtcga cgagcgggcg gcggggagcc tcgacacggc cgtgctgctc gaggcggtcg
    23161 gcaacccgtc ctcggccggt ctcgccgtcg ccgaggccga ggcgtcccag ctcgccgcca
    23221 gcatcgactg ggatgcgcgg gcggcccgca cccgcaccga gcgcgagctg gtcgagcggg
    23281 tcggccagat gttccgctcg gccctcgggc cgctcgggta ggcgctcagt cccggaagtt
    23341 ctcgaactgg agggggacct cgaagtcccc ttcccggagg gcggcgatga cctcctgcag
    23401 cgagtcgcgg ctcttgccgg tgacccgcag ctgctcgccc tgggtctggg actggatgcc
    23461 cttcacgccc aggcccttga tgaacttgtt gagctcgcgg gccttgtccg acgtgatccc
    23521 ggcccggagc gcgatggtct ggcgggccga ccccccggcg gcctcctcga ccttgccggg
    23581 atcgaggacc ttgaggctga ccttgcgctt cacgagcttc tcctcgagca ccgtgcgcag
    23641 cgcgtgcagg cggtcctcgc tgccggaccg gagggtgatg ctctcctcac cgctcagctc
    23701 gatcgagctg tcggtcccct tgaagtcgta gcgggtggat gcctcgcgag cggtctggtc
    23761 gaccgcgttg cggacctcct gcacgtcgaa ctggctgacc acgtcgaagc tgggcatggg
    23821 tgcagagact agcgatggtc gcttgggccc cggccaggtg ccgctgctac cttgagcgcc
    23881 ccctgggtcg gtgcccgagt ggccaaaggg aacgggctgt aaacccgtcg gcgtacgcct
    23941 tcgttggttc aaatccaacc cggcccacgt ttggaaactg gcagtcacag cgtcacgcgc
    24001 agtcatgtgt gacgctgtga ctgccagttt ctcttacagc agcagctgca tgacggtgac
    24061 gtcgagccag cggccgaact tgcgaccgac ctcgcgctcg accccgacgt gctcgaagcc
    24121 caccgcggcg tggagccgga tcgaggcggc gtgatcggcc acgatgcggg ccatcacggc
    24181 gtggaagcca cgctcgcggg ccacgccgac gatctcctcg agcagggccc ggccgacgcc
    24241 gctgccccgg tggtcggcgt gcacgtagat cgagtcctcg accgtggtgc ggtacgccgg
    24301 ccggtcccgg tagggcgaca gcgacgcgaa cccggccacc tcgccgtcca cctcggccac
    24361 gaccacctcg agcgcccccg accgcgaccg gagccacgcc gactgctcct ccaccgtgcg
    24421 ggtcaccagg tcgaaggtga cgcgcgaccc cgtcacctcg gcgttgtaga tggcagcggt
    24481 ggcggcggcg tcggccgggg tggcggggcg caggagcacg cccatcatgg tggcgtgctc
    24541 aggcggaggg ggcggcgatg gcgccgtcgt cgatcgtcgg ctccaccacc gacatgcggc
    24601 cccggcgggc ctcgtcccga gcccaggcga cgacgtcctc gtcggaggtc acgaccacga
    24661 gctgggggtc gccggcgtgc gccgccagca tctcgagcag caggggcttc accgcagcgt
    24721 cgagcccctc gaagggatcg tccacgatca gcggcagcga gtccccgggg gtcagcgccc
    24781 gcaccgcccg gacccgggcg aggatcgcct gggcgagctc ggcggacacg tcgtcgcccg
    24841 ccgcaccgac ggatgcactc gtatgggcac cgagcccggc gtgcagctcc gcggccgctc
    24901 ggatctcgcg ctcgtgctcg agggcgaaga gcagcggcgc cggtcccgcc acctgggccc
    24961 aggccgccat ggcccggcgg tggtcgccca cggcccgcat gaacgcccgg cgctcgtggt
    25021 cggtgtccaa cagggcgctg acccgctcgt agtggaacgc ggcgtagtcg tcggcaccgg
    25081 cctggcgcag cacgttgcgc tcggccttgg cggcgtcgtc caccgatcgc cggaagcgga
    25141 ggccgagggc caggccgaac agggcgatga gcaggaaggg gagcgcggcc ggcgagccct
    25201 ccaggttgag catcccgacg gcaccgaggg ctccgaccgt gccgatggtc agggcgatca
    25261 ggcggacccg ctcgtagctg tccgtcgcgg cgacgagggc ggcgtggcgc tcctcgatct
    25321 cgcgcacgat cggggcgtcg tccatggaca caccgccacc ggcggaggcc tgctcgagca
    25381 gccgctccga tgctcggcac cgcatggcgg tgtcccacag cgcctcctgg tcggcggcgc
    25441 cgagccgggc gatccaggcg tcggactcgt gctcgggcac caggtcgtcg ctcgtgaagc
    25501 ggatcgtgcg cctggccagg gcccgatcga cgccggcgga ggcgaacagg tcgatctccc
    25561 cgtcggcgcc gaggtggtcc tcggtgacgt cgcgcaccga gtcggtgtcg atcacgcggt
    25621 ggcgacccgt ctcaggccgg aagacggtga ggctgcgacc gccggcctgc acctcgaggt
    25681 gcacgcccgg gcgggcgccg gccagcgcgt cgatgacctc gccggcgagg gcctcacggg
    25741 tgctcggcga caggccgcgg atcaccgtga gctccgggtg gaagtcggcg ctgagcgtgt
    25801 gctcgcccga gccgatcacg agccgttcca gtcgcaccgg aggctcatcg gcgcatcggg
    25861 gcgccaactt gagccggacc tgggccaaaa gccccacgcc agcctcgatc tttgcgtcgg
    25921 gggcggccgt gcgtagactc tcccgccgcg ccagaaccgg ctctcgggcc gggtccagcg
    25981 tggccgcaac accgccccgt tagctcagtc ggcagagcac agccatggta aggctggtgt
    26041 cgtcggttcg attccgacac ggggctcgac accactgccg ctccggcggt ggcgccacca
    26101 gggcgacgta gctcagttgg ttagagcact cggctcataa tcgagttgtc gtcggttcga
    26161 ttccgaccgt cgccacggac tgcgagtcgc tcgcaatcga tcacgacacc gtttccggcg
    26221 cccggcaccc cggccgccac cgacagcgaa gaggaagcac ggccatggcc aaggagaagt
    26281 tcgatcggtc gaagccccat gtgaacatcg ggacgatggg tcacatcgat catgggaaga
    26341 cgacgttgac ggctgcgatc tcgcgggtgt tgtcggagcg tgttgatggg aacaacgcga
    26401 cggccttcga catgatcgac aaggcgcccg aggagcggga gcgtgggatc acgatcaatg
    26461 tggctcacat cgagtacgag acggacaagc ggcattacgc gcatgtcgac atgccgggtc
    26521 acgcggatta catcaagaac atgatcacgg gtgccgcgca ggtcgatggt gcgatcttgg
    26581 tggttgctgc gacggatggt ccgatgccgc agacccgtga gcatgtgttg ttggctcgtc
    26641 aggttggtgt gccggcgatc gtggtggcgt tgaacaaggt cgacatggtc gatgatgagg
    26701 agctgttgga gctggtggag atggaggtgc gtgagctcct ctctgatcag gacttcgatg
    26761 gtgacaacgc tccggtggtg ccggtgtctg cgttgaaggc gttggagggt gactctgatg
    26821 ctggtgatca ggtgatcgag ctgatgaacc aggtggatga gtacatcccc gagccggagc
    26881 gtgatctgga caagccgttc ttgatgccga tcgaggatgt gttctcgatc cagggtcgtg
    26941 gcacggtggt gacgggcaag gtggagcagg gtgtgatcca tgtcggtgac gacatcgaga
    27001 tcgtgggcat tcgtgacacg cagaagacga cgtgcacggg tgtggagatg ttccgcaagc
    27061 tgttggatca gggtcaggct ggtgacaaca tcggcgccct tcttcgtggc atcgacaagg
    27121 acggggtgga gcgtggccag gtgctgtgcg ccccgggcag catcacgccg cacaccgagt
    27181 tcgaggcgac ggtgtatgtg ttgaccaagg acgagggtgg ccgtcacaag ccgttcttct
    27241 ccaactaccg gccccagttc ttcttccgga ccaccgacat cacgggttcg atcgagctgc
    27301 c
//
LOCUS       DATISU010000174         7951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_520595, whole genome shotgun sequence.
ACCESSION   DATISU010000174 DATISU010000000
VERSION     DATISU010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7951
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520595"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..963)
                     /locus_tag="VLR27_07215"
     CDS             complement(16..963)
                     /locus_tag="VLR27_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP03271.1"
                     /translation="MRLQRIIGRLVVLVGVLLAVSFLTFAMLNVLGGDPALAIVGADN
                     ADQETLTKVRDELGLNDPFLVQYKDWVTGVVQGDLGRSYKTKQPVAEAIGERVPVTAE
                     IGLLSMLIALAVSVPLAVWTSYKAGTKVDKGTSAITFGLLSIPNFLLAYWLGYIVAVK
                     FGWLPRSGWVRLTADPLENLRTAILPALSLAIAEMAVYTRILRSDMIQTLQEDYLLMA
                     RSKGLSTKRILFMHALRPSTLSLMTVIGVQFGALIGGSIIVEQIFALPGMGNYLLNAI
                     YERDYIVVQGVVVVIAASFVIVNFAVDIVYRLLDPRIKV"
     gene            complement(1003..2739)
                     /locus_tag="VLR27_07220"
     CDS             complement(1003..2739)
                     /locus_tag="VLR27_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSP03272.1"
                     /translation="MTPFRRRLLALLTVLSVLLTACGGDDDDGAAEDTTEDSEEGDGV
                     RENAEDEEAAADEPQYGGSITIGLEAETNGWLPGTSQPANAGYTVYSAIYDPLVVRVE
                     DGSIEPYLAETVEPNEDYTRWSFTLREGITFHDGTDLTAEVIKRNWDEYLTAEGANTA
                     GALEGITVEVTGPLAYDYVLEEGDAAFLDTLTGTAGYPFSVEAAVAAGEDAGANPVGT
                     GPFTFGSWTRDGQLVVNRNENYWRTDESGNQLPYLDSITFRPIPDEDTRLDSVASGDI
                     DAMQTLRQSIVRRARELVDGGGYNSFEWLGNNGGAGIINTMVPPFDDKRVRQALVYAV
                     DQEQMIEVLGGTGITPPQTQWFTEDSPWYSEAAADAWYDYDPDKATELLDEYINDPER
                     SDGQAPGTPISFTYDCPPDPSLVELSQAYQAFWQQVGLEVNLDSKEQSAHITQGIGTE
                     NEPFLRGDYEVQCWRLGSQADPYSVWSSAFGPVEAEVLNVSDWTHPDVDALLEELRVE
                     GDTDERKRIVEEISLILAEEVPNFWTGGTATAIVAREQVRGILGGVTPNGTPIDGASD
                     AILRTPYLWLEQ"
     gene            complement(2847..3476)
                     /locus_tag="VLR27_07225"
     CDS             complement(2847..3476)
                     /locus_tag="VLR27_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03273.1"
                     /translation="MDLASARRTLGVRAEDDWLVIRDTYREQIRRSHPDIAGEAGAAR
                     SVELNAAYRTLAVARRQGRLHEADAPAPPPAGPSQPPSPRAAGDPPLAVRPEWAPPVG
                     PAADVRRLDHDTLVLSSTPDESFRRLVEATHEIGEISFLDRSSAIFEAVLTLDDGTAA
                     SFVVSLQWRAHDATCEAFCTLEALGRPQHLDVGGVLEQLAAYIGPDQMG"
     gene            complement(3483..4778)
                     /locus_tag="VLR27_07230"
     CDS             complement(3483..4778)
                     /locus_tag="VLR27_07230"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HSP03274.1"
                     /translation="MPETAAPDRAVADELCRFIDDSPSPYHAVATAAALLEEAGFEEA
                     RLDQPLPGGAGGRYVRRGGSLVAWLQPADGVRAFRIVGAHTDSPSLRIKPRPDHVTAG
                     VRQLGVEVYGGALLNSWLDRDLGLSGRVSVSTPDGPQVRLVRIDEPLLRVPQLAIHLD
                     REISTDGLKLNPQTHLAPMWDLDGDRGSGAGFQGLLAERLDVGAGDILAWDVMAHDLT
                     PSRRTGVDGVFVSAPRLDNLCSSFAGLHGLLAAPEDSPAVPVVCLYDHEEVGSTSSTG
                     ADGALLVSVLERIALAAGTDRAGLHAALAGSLCVSADMAHATHPNYADRHEPEHHVAL
                     NAGPVVKVNANQRYASDAESVGILLQACADAGIPTQRFVTRTDLPCGSTIGPVTAARL
                     GIPTVDIGVAQLAMHSARELCGADDPSWYVATMAAALQG"
     gene            4841..5719
                     /locus_tag="VLR27_07235"
     CDS             4841..5719
                     /locus_tag="VLR27_07235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSP03275.1"
                     /translation="MAASPTTVVVVHRDRPRLLAATLEALAAQTVPVQVLLVDSGSTE
                     EHHREALALLPPGSDVVRMGRNGGFGPSANAGWRRFLADHEGEWVGLCPHDALPAPDA
                     IERILAAATARPRAGFACGDYGDGCTPVVDPYFGSILVPATVEEGWEPAGYPHGTLLL
                     ARRGALEDVGLFDERYFAYCEEADLGLRAAAAGWEVGLVRGARVFNPNMSNPRPVIDY
                     LMVRNTLLLVRSHSGRYKAFIRFCIAVAQLWTERRRSPYHHTQARLHALVDFLRGRWG
                     PPPARLVGAGKHPDEP"
     gene            complement(5728..7482)
                     /locus_tag="VLR27_07240"
     CDS             complement(5728..7482)
                     /locus_tag="VLR27_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSP03276.1"
                     /translation="MQPSSDHPTDAEGSNALRRYGPIVAIVAVIAIVIGLVVAGGGDD
                     DDDEAAATVDDTAEETTSEDGSETDTGSTELEGAISWTRAQDEGLDVTFPDTCDPETG
                     QAAIPYFFAAECYADVEDNGGATSQGVTEDTITVVYYSDPETDAVLDFITGPIQNDDT
                     QAQVEETVQGFMELFGTYYQSYGRTVDLKIVRGSGASTDEVAARADAQAAIEEHKPFA
                     AFGGPTLTTAWADEMAAAGVVCISCGSTGSEEYRDARAPHLIGIGMAGSQINLHGAEY
                     ISTKLAGKPAEFGGDDVVGQERVFGHVYISSSEESERSAGQFADMLSERGVELAEQLS
                     YQLDPGRLPEQATSVISKLKESGVTSVIIQADPVAPANFTQEATAQEYFPEWVVAGGT
                     LVDTNTFGRIFDQAQWEHAFGITTNAVPLDPTTTPGFALYEWFHGSPPPADQTAPILF
                     PSPSLLHSIIQAAGPNLTPETFRDGLFAVTPTQRSISQPSLSWGDHGLWDDLDTENDW
                     SGVDDVSEWWWDPTATGPDEIQREGTGMMRFVAGGKRYLPGEWDDTVALFDPADTVTL
                     FDEPPADERAPEYPSPAG"
     gene            complement(7510..>7951)
                     /locus_tag="VLR27_07245"
     CDS             complement(7510..>7951)
                     /locus_tag="VLR27_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSP03277.1"
                     /translation="NAYDAEQKVARRVEELVELLGLGAYRSKFIRELSTGTRRVVDLA
                     GLLAHRPSVILLDEPSSGIAQREVEALGPMLERIRRETGASLIVIEHDMPLLRSVSDR
                     LVAMDQGAVIATGAPDAVLEDPTVIESYLGPDDTAIHRSTITST"
BASE COUNT         1054 a         2681 c         2988 g         1228 t
ORIGIN      
        1 gacgccagcc atggttcaga ccttgatccg ggggtcgagc agccggtaca cgatgtcgac
       61 cgcgaagttg acgatgacga acgaggcggc gatcacgacg accacgccct ggaccacgat
      121 gtagtcccgc tcgtagatgg cgttgagcag gtagttgccc atgcccggga gggcgaagat
      181 ctgctcgacg atgatgctgc cgccgatgag cgccccgaac tggacgccga tcacggtcat
      241 gagcgacagc gtcgaggggc gcagggcgtg catgaacagg atccgcttcg tgctcagccc
      301 cttggaccgg gccatgagga ggtagtcctc ctgcagggtc tggatcatgt cgctgcgcag
      361 gatgcgggtg tagacggcca tctcggcgat ggccagcgac agggcgggca ggatcgccgt
      421 gcgcaggttc tcgagcgggt ccgcggtgag gcggacccac cccgatctcg ggagccaccc
      481 gaacttcacc gccacgatgt agcccagcca gtaggcgagc aggaagttgg ggatcgacag
      541 caggccgaag gtgatggcgc tggtgccctt gtcgaccttg gtaccggcct tgtacgaggt
      601 ccacaccgcc aggggaaccg acacggcgag agcgatgagc atcgacagca gcccgatctc
      661 ggccgtgacg ggcacccgct cgccgatggc ctcggccacg ggctgcttgg tcttgtacga
      721 ccggccgaga tcgccctgca cgacgccggt gacccagtcc ttgtactgca cgaggaacgg
      781 gtcgttcagc cccagctcgt cgcggacctt ggtgagcgtc tcctggtcgg cgttgtcggc
      841 tccgacgatg gccagggccg ggtcgccgcc gagcacgttg agcatggcga aggtgaggaa
      901 ggagacggcg agcagcacgc ccaccagcac gaccagccgg ccgatgatcc tctgcagcct
      961 catgccgtct cctccctcgt caccttcacc tgctccgttc ggctactgct cgagccagag
     1021 gtacggggtg cgcaggatgg cgtcggacgc accgtcgatc ggcgtgccgt tcggcgtgac
     1081 gccgccgagg atcccccgca cctgctcccg ggcaacgatg gccgtggccg tgccgccagt
     1141 ccagaagttc gggacctcct cggccaggat cagcgagatc tcctcgacga tccgcttgcg
     1201 ctcatccgtg tcgccctcga cccgcagctc ctcgagcagc gcgtcgacgt cggggtgcgt
     1261 ccagtcgctc acgttgagca cctcggcctc caccggcccg aaggcgctgg accacaccga
     1321 gtacggatcg gcctggctgc cgaggcgcca gcactgcacc tcgtagtcgc cccggaggaa
     1381 cggctcgttc tccgtgccga tgccctgggt gatgtgggcc gactgctcct tggagtcgag
     1441 gttcacctcg aggcccacct gctgccagaa ggcctggtag gcctggctga gctcgaccag
     1501 cgacgggtcg ggcgggcagt cgtaggtgaa gctgatcggc gtgcccgggg cctggccgtc
     1561 ggaccgctcg gggtcgttga tgtactcgtc gagcagctcg gtggccttgt ccgggtcgta
     1621 gtcgtaccag gcgtcggcgg cagcctcgga gtaccagggg ctgtcctcgg tgaaccactg
     1681 ggtctggggc ggggtgatgc cggtgccgcc gaggacctcg atcatctgtt cctggtcgac
     1741 ggcgtagacg agcgcctggc gcacccgctt gtcgtcgaag ggcgggacca tcgtgttgat
     1801 gatgccggcg ccgccgttgt tgcccagcca ctcgaagctg ttgtagccac cgccgtcgac
     1861 gagctcacgg gcccggcgga cgatgctctg gcgcagggtc tgcatggcgt cgatgtcgcc
     1921 ggaggcgacg ctgtccaggc gggtgtcctc gtccgggatc ggacggaacg tgatcgagtc
     1981 gaggtacggg agctggttgc cgctctcgtc ggtgcgccag tagttctcgt tgcggttcac
     2041 gacgagctgg ccgtcccggg tccaggaccc gaaggtgaag ggcccggtgc ccaccgggtt
     2101 ggcgccggcg tcctcgccgg cggccacggc ggcctcgacg gagaacgggt agcccgcggt
     2161 gccggtgagg gtgtcgagga aggcggcgtc gccctcttcg agcacgtagt cgtaggcgag
     2221 tggcccggtg acctccacgg tgatgccctc gagcgcaccg gcggtgttgg cgccctcggc
     2281 ggtgaggtac tcgtcccagt tgcgcttgat gacctctgcg gtcagatcgg tgccgtcgtg
     2341 gaacgtgatg ccctcgcgga gggtgaagga ccagcgggtg tagtcctcgt tgggctcgac
     2401 cgtctcggcg aggtagggct cgatggagcc gtcctcgacc cggaccacga gtgggtcgta
     2461 gatggcggag tacaccgtgt agccggcgtt ggccggctgg gacgtgccgg gcagccagcc
     2521 gttggtctcg gcctcgaggc cgatggtgat cgatccgccg tactggggtt cgtcggcggc
     2581 cgcctcctcg tcttcggcgt tctcccggac gccgtcgccc tcctcggagt cctcggtggt
     2641 gtcctcggcg gcgccgtcgt cgtcatcacc cccgcaggcg gtgagcagca ccgagagcac
     2701 ggtgagcagt gcgagcaacc gacgtcggaa cggcgtcatg caatcaatcc ccctggttcg
     2761 ggccgacggt cccccgaccg ccggtgtgac gccggacact agccacgctt gaccctgcgg
     2821 tcaggcaccg tggcgaaata tccccttcat cccatctggt cgggaccgat gtaggcggcc
     2881 agctgctcca ggacaccgcc cacgtcgagg tgctggggtc ggccgagggc ctcgagcgtg
     2941 cagaacgcct cgcaggtcgc gtcgtgcgcc cgccactgga ggctgaccac gaacgacgcc
     3001 gccgttccgt cgtcgagggt gaggacggcc tcgaagatgg ccgagctccg gtccaggaag
     3061 gagatctccc cgatctcgtg ggtggcctcg accaggcggc ggaacgactc gtccggggtg
     3121 ctggacagca cgagcgtgtc gtggtcgagg cgacggacgt cggcggccgg ccccacgggc
     3181 ggggcccact ccggtcgcac cgccaggggc gggtcgccgg cagcccgggg cgacggtggt
     3241 tggctcggcc cggccggcgg cggtgcgggc gcatccgcct cgtggaggcg gccctgccgc
     3301 cgggcgaccg cgagcgtccg gtaggcggcg ttcagctcga cgctccgagc cgcccccgcc
     3361 tccccggcga tgtcgggatg cgaccgcctg atctgctcgc ggtaggtgtc gcggatcacc
     3421 agccagtcgt cctcggcgcg cacgcccagc gtgcgacgcg ccgaggccag gtccacgcgg
     3481 cctcagccct gcagcgccgc agccatcgtg gccacgtacc agctcgggtc gtcggcgccg
     3541 cacagctcgc gtgccgagtg catggcgagc tgggcgaccc cgatgtcgac ggtggggatg
     3601 ccgaggcgag cggcggtgac cggtccgatc gtcgacccgc acgggaggtc ggtgcgggtg
     3661 acgaagcgct gggtcgggat gccggcgtcg gcgcaggcct gcaggaggat gccgaccgac
     3721 tctgcgtcgg aggcgtagcg ctggttggcg ttcaccttca ccaccgggcc ggcgttcagg
     3781 gccacgtggt gctcgggctc gtggcggtcg gcgtagttgg ggtgcgtggc atgggccatg
     3841 tcggccgaca cgcacagcga gccggcgagg gcggcgtgga ggccggcccg gtcggtgccg
     3901 gcagcgaggg cgatgcgctc gagcaccgag acgaggagcg cgccgtctgc acccgtcgag
     3961 ctggtcgaac ccacctcctc gtggtcgtag aggcacacga ccggcacggc cggggagtcc
     4021 tcgggcgcgg cgagcagccc gtggaggccg gcgaaggacg agcacaggtt gtccaggcgg
     4081 ggcgcgctca cgaacacgcc gtcgacaccg gtgcggcgtg acggcgtcag gtcgtgggcc
     4141 atcacgtccc aggcgaggat gtcgccggca cccacgtcga ggcgctcggc gagcagcccc
     4201 tggaacccgg ccccggagcc ccggtcgccg tcgaggtccc acatcggcgc cagatgggtc
     4261 tgcgggttca gcttgagccc gtcggtgctg atctcgcggt cgaggtggat ggcgagctgg
     4321 ggcacgcgca gcagcggctc gtcgatccgc acgagccgga cctgcggacc gtccggggtg
     4381 gacaccgaca cccggccgga gagccccagg tcgcggtcga gccacgagtt cagcagcgct
     4441 ccgccgtaga cctcgacgcc cagctggcgg accccggcgg tgacgtggtc ggggcggggt
     4501 ttgatgcgca ggctcgggct gtcggtgtgg gcccccacga tccggaacgc ccggaccccg
     4561 tccgccggtt ggagccaggc gacgagcgac ccgccccggc ggacatagcg gccgccggcc
     4621 ccgcccggga gcggctggtc gagccgcgcc tcctcgaagc cggcctcctc cagcagcgcc
     4681 gccgcggtgg cgacggcgtg gtacggcgag ggcgagtcgt cgatgaaccg gcacagctcg
     4741 tcggccacgg cgcggtcggg ggcggcggtc tcgggcatgc cccgatgctg gcacgccgcc
     4801 ggtttgggcc gtggttcggg acggccgaca ggatgagccc gtggcagcgt ccccgacgac
     4861 cgtggtggtc gtgcaccgcg atcgaccccg gctcctcgcc gccaccctgg aggcactggc
     4921 ggcccagacc gtgccggtgc aggtgctgct ggtcgactcc ggctccaccg aggagcacca
     4981 ccgcgaggcg ctggccctgc tgccgcctgg gtccgacgtg gtccggatgg gtcgcaacgg
     5041 tggcttcgga ccctcggcga acgccggctg gcggcggttc ctcgccgacc acgaggggga
     5101 gtgggtcggc ctctgccccc acgacgccct gccggccccc gatgcgatcg agcggatcct
     5161 cgccgccgcc acggctcgac cccgcgccgg cttcgcctgc ggcgactacg gcgacggatg
     5221 cacccccgtg gtcgacccgt acttcggctc gatcctcgtg ccggcgaccg tggaggaggg
     5281 ctgggagccg gccggctacc cgcacggcac cctgctgctc gcccgccggg gcgcgctcga
     5341 ggacgtcggg ctgttcgacg agcggtactt cgcgtactgc gaggaggccg acctcggtct
     5401 gcgggcggct gctgccggct gggaggtcgg cctggtccgt ggcgcccggg tgttcaaccc
     5461 gaacatgtcc aacccccgcc cggtcatcga ctacctgatg gtgcgcaaca cgctgctgct
     5521 ggtgcgctcg cactcgggcc ggtacaaggc gttcatccgg ttctgcatcg cggtcgccca
     5581 gctgtggacc gagcgccggc gcagcccgta ccaccacacc caggcccgcc tgcacgcgct
     5641 ggtggacttc ctgcggggcc ggtgggggcc gccgccggcg cggctcgtcg gggcgggcaa
     5701 gcaccccgac gagccgtagg agggcgatca gccggcgggg gaggggtact ccggtgcccg
     5761 ctcgtcggct gggggttcgt cgaacagcgt caccgtgtcg gccgggtcga agagggcgac
     5821 ggtgtcgtcc cactcgccgg ggaggtagcg cttcccgccg gcgacgaagc gcatcatgcc
     5881 ggtgccctcg cgctggatct cgtccgggcc ggtggccgtc gggtcccacc accactccga
     5941 cacgtcgtcg acgccgctcc agtcgttctc ggtgtcgagg tcgtcccaga ggccgtggtc
     6001 gccccaggac agggacggct gcgagatgga ccgctgcgtg ggcgtgacgg cgaacagccc
     6061 gtcgcggaac gtctccggcg tcaggttcgg gcccgcggcc tggatgatcg agtgcagcag
     6121 cgacgggctg gggaacagga tcggcgcggt ctggtccgcc ggcggcgggg agccgtggaa
     6181 ccactcgtag agcgcgaagc ccggggtcgt ggtcgggtcg agcggcacgg cgttcgtcgt
     6241 gatgccgaag gcgtgctccc actgggcctg gtcgaagatc cgcccgaagg tgttggtgtc
     6301 cacgagggtg ccgccggcga cgacccactc ggggaagtac tcctgcgcgg tggcctcctg
     6361 ggtgaagttg gccggcgcga ccgggtcggc ctggatgatc accgaggtga cgccggactc
     6421 cttcagcttc gagatgacgc tggtcgcctg ctcggggagg cggccggggt cgagctggta
     6481 gctgagctgc tcggccagct ccacgccccg ctcgctgagc atgtcggcga actggccggc
     6541 gctgcgctcg gactcctccg agctgctgat gtacacgtgg ccgaagacgc gctcctggcc
     6601 gacgacgtcg tcgccgccga actcggccgg cttccccgcc agcttggtcg agatgtactc
     6661 ggcgccgtgg aggttgatct ggctgccggc catcccgatg ccgatgaggt ggggggcccg
     6721 ggcgtcccgg tactcctccg agccggtcga gccgcaggag atgcacacga cgccggccgc
     6781 agccatctcg tcggcccagg cggtggtgag cgtcggcccg ccgaaggcgg cgaagggctt
     6841 gtgctcctcg atggcggcct gggcatcggc ccgggctgcg acctcgtcgg tggaggcgcc
     6901 gctgccccgg acgatcttca ggtccaccgt gcggccgtag ctctggtagt aggtgccgaa
     6961 gagctccatg aagccctgga cggtctcttc gacctgggcc tgggtgtcgt cgttctggat
     7021 cggcccggtg atgaagtcga gcacggcgtc ggtctcgggg tcggagtagt agacgacggt
     7081 gatggtgtcc tcggtgacgc cctgggaggt ggcgccaccg ttgtcctcca cgtcggcgta
     7141 gcactcggcg gcgaagaagt acgggatcgc ggcctggccg gtctcgggat cgcacgtgtc
     7201 cgggaaggtc acgtcgaggc cctcgtcctg ggcacgggtc cagctgatcg ctccctcgag
     7261 ctcggtcgag cccgtgtcgg tctccgagcc gtcctccgag gtggtctcct cggcggtgtc
     7321 gtccaccgtc gcggccgcct cgtcgtcgtc gtcatctccg ccgccggcca cgacgaggcc
     7381 gatgacgatg gcgatcaccg cgacgatggc gacgatcggt ccgtagcgac gcagggcgtt
     7441 gctgccttcc gcgtccgtgg ggtggtcgct gctgggttgc atgccggtgc tcctgtgggt
     7501 cggtggggtt caggtggagg tgatggtcga gcggtggatg gcggtgtcgt cgggtcccag
     7561 gtaggactcg atgaccgtcg ggtcctcgag gacggcgtcg ggcgctccgg tggcgatgac
     7621 ggcgccctgg tccatggcga cgaggcggtc ggacacgctg cgcagcagcg gcatgtcgtg
     7681 ctcgatgacg atcaggctgg ctccggtctc gcgccggatg cgctcgagca tcgggccgag
     7741 ggcctcgacc tcgcgctggg cgatgccgga ggagggctcg tcgagcagga tcaccgaggg
     7801 tcggtgggcc agcaggccgg ccaggtccac gacgcgcctc gtgccggtgg acagctcccg
     7861 gatgaacttg gaccggtagg cgccgaggcc gaggagctcg accagctcct ccacccgccg
     7921 cgccaccttc tgctccgcgt cgtacgcgtt c
//
LOCUS       DATISU010000175         6097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_752954, whole genome shotgun sequence.
ACCESSION   DATISU010000175 DATISU010000000
VERSION     DATISU010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752954"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1509)
                     /locus_tag="VLR27_07250"
     CDS             complement(<1..1509)
                     /locus_tag="VLR27_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HSP03278.1"
                     /translation="MAATRSSQKKKPPAKRRSTAKKPAKAPSRVGGTVGAALDGRSAD
                     VAGLTLIGIGIIAGAGLYADVAGPVGQLLASASSGFAGLAGVLVPPVLVLVGIACMRD
                     NDPATGQGLFGRAHLVVGGVLLAVAATGLLHVLRGQPSFDAGVDEWGAAGGLLGMGTG
                     APLVAVAATGGATVALLTLLAISFFVITRIPVRVAAEGAVSTFRPLGETLRATARSLF
                     TLGDEPDREGDRGPDVTVAGEPTTIYDLDDEPEPLPPEPVDETRADPVVEIPAATEAP
                     TELDLRPTAPVGDWKLPPAKVLVRTGSQEVDRKAVEEDGRRLEASLAQHGVETRLIGM
                     VVGPTVTRYELELGPGVKVNKVTSLAKDIAYNMATPDVRILAPIPGKQAIGIEVPNAR
                     RQLVTVGDILTSREAVEAEHPLEVAVGRDINGKSHLMNLAQMPHILIAGATGAGKSSC
                     LNSILTSILMRSTPDQVRMILIDPKRVEMGQYEGLPHLLTQVVTNPKKAANAL"
     gene            complement(1674..3500)
                     /locus_tag="VLR27_07255"
     CDS             complement(1674..3500)
                     /locus_tag="VLR27_07255"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HSP03279.1"
                     /translation="MLDSEITNPTDNPQFQDVLDARLSRRSVLGGGVAAAAFSFFGTG
                     IAGAARPSSAPGGGASAANRELLAFRPVPLGFGDEIVVPKGYTATAFLPWGTPILPGA
                     GSGTFPTDAEDQEKRIGLGHDGMWFFPTGKHGERGLLCINHEYGTDPHITNGTGIQSG
                     TPEMVAISQAVHGISVVEIERVRNEWRVVMGPKNRRVTGTKGAAVFTGPAAGSALLEN
                     PADNEAAGTLNNCGNGKTPWGTYLTCEENFNGYFGTEKPLGARTESEERYGLTATGFG
                     YRWHLHDQRFDLRDPAHVNEANRYGWIVEIDPMAPSSPIQKHTALGRFKHENVELVEA
                     PDGRVVAYMGDDQADDYVYRYVSARPWRHMRSAGVSPLSDGTLSVARFDADGTGRWLP
                     LELDPVNAARTDDGNAPFADMAELVVNVRLAADALGATKMDRPEWVAANPVNGDVYFT
                     CTNNTGRTSADAANPRTQNRWGHIIKFVDPSHTASRFDWDIFLLAGPAGSGATIDPED
                     AFGSPDGLWIDESGRMYIQTDGAQPEGANDQMLVADPRNGEIRRFLTGVPGCEVTGIT
                     PAGKDDLFVNIQHPGNGDPGTSSFPDGGGSVPRDCTVVITRG"
     gene            complement(3647..5302)
                     /locus_tag="VLR27_07260"
     CDS             complement(3647..5302)
                     /locus_tag="VLR27_07260"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HSP03280.1"
                     /translation="MPAPVKITFLGGLGEIGRNCACIEVDGRIMLIDCGLMFPDLDML
                     GVDLVLPDFSYLYDNADRVEGAVLTHGHEDHTGGLSFLLRDISFPLYGSELTLGFAKN
                     RIEEAGLLDRTSFNIVKDGERRKVGPFDLEFIPATHSVPHGFATAFHTPQGTILHSGD
                     FKIDLTPVDGRLTDLTRIGAMAHDPGIRLLLADSTNAEERGHSASETSVGRVLYDLFH
                     AHEGRRIITACFASHVHRVQQIADAAVAFDRKIATLGMSMKKNVKLARSLGLLNIPES
                     SLIDIEQIDKHDPGKVCIISTGSQGEPMSALALMAATESRWLTLTPDDTVILSSHPIP
                     GNEMNVSKVIDGLIRQGVEVVYSGIADVHATGHAKAEELKTYHSIVKPEWFIPVHGEY
                     RHLVAHAKIARAMGTAQDHVWVCEDGHQLELTDDGLRAVGKVPAGYLYVDGTIGDVGQ
                     GVLRDRKVLAEEGVVVVIVTVDVETGAVLNRPEIVTRGWIYEKEAEGLLTECANEVEK
                     AVAEVKDATDIEAVQRAARRAAGRFVNRETKRKPMIVPVVLEA"
     gene            complement(5327..>6097)
                     /gene="dapA"
                     /locus_tag="VLR27_07265"
     CDS             complement(5327..>6097)
                     /gene="dapA"
                     /locus_tag="VLR27_07265"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HSP03281.1"
                     /translation="SDGLVVNGTTGESPTLSATESVDLFRAVRQAVDVPVIAGTGSND
                     TAHAIRQSEAAAGIGVDGLLVVAPYSNKPSQAGLLRHFRMIAEATDLPIVLYDIPGRT
                     SRKVDSDTILTLAHEVPTIVGLKDAAGSPAETAGVIAHAPEDFDVYSGDDNLTLPLLS
                     VGARGAISVASHWVGDQIRAMFDAFDAGDVAEATRINQTLLPSYAFESTLDAPNPVPT
                     KAMLRTLGLPAGECRPPMGPTPDGLEVQAKELWDSLGT"
BASE COUNT          822 a         1976 c         2332 g          967 t
ORIGIN      
        1 cagcgcgttg gccgccttct tcgggttggt cacgacctgg gtgagcaggt ggggcaggcc
       61 ctcgtactgg cccatctcga cccgcttggg gtcgatgagg atcatgcgca cctggtccgg
      121 cgtggagcgc atgaggatcg aggtgaggat cgagttgagg cagctggact tgcccgcacc
      181 cgtcgcaccg gcgatgagga tgtggggcat ctgggccagg ttcatcaggt gcgacttgcc
      241 gttgatgtcg cggccgacgg cgacctcgag ggggtgctcg gcctcgacgg cctcgcggga
      301 ggtgaggatg tcgccgacgg tgacgagctg gcggcgggcg ttgggcacct cgatgccgat
      361 ggcctgcttg cccgggatcg gcgccaggat gcgcacgtcg ggcgtggcca tgttgtaggc
      421 gatgtccttg gccaggctcg tgaccttgtt gaccttgacg ccggggccga gctcgagctc
      481 gtagcgcgtg acggtggggc ccacgaccat gccgatcagg cgggtctcca cgccgtgctg
      541 ggccaggctg gcctcgaggc ggcgcccgtc ctcctccacg gccttgcggt cgacctcctg
      601 gctgccggtg cgcacgagca ccttggccgg cggcagcttc cagtcgccga ccggtgcggt
      661 ggggcgcagg tcgagctcgg ttggtgcctc ggtggcggcc gggatctcga cgaccgggtc
      721 ggctcgggtc tcgtccaccg gttcgggggg cagcggctcg ggctcgtcgt cgaggtcgta
      781 gatcgtggtg ggctcgccgg cgacggtgac gtcggggccg cggtcgccct cgcggtcagg
      841 ctcgtcgccc agggtgaaga gcgaccgtgc cgtggcccgc agggtctcgc cgagcgggcg
      901 gaacgtggac acggcgccct cggcggcgac ccggaccggg atgcgggtga tgacgaagaa
      961 cgagatggcc agcagcgtga gcagcgccac cgtcgcaccg ccggtggcgg cgacggcaac
     1021 cagcggggca ccggtgccca tgccgagcag gccgccggcg gcaccccact cgtccacgcc
     1081 ggcgtcgaag ctgggctggc cgcgcagcac gtggagcagc ccggtggcgg ccacggccag
     1141 gaggacgccg ccgaccacga ggtgggcccg gccgaacagg ccctgcccgg tggccgggtc
     1201 gttgtcgcgc atgcaggcga tgccgacgag caccagcacg ggtgggacga gcacgccggc
     1261 caggccggcg aacccggacg aggcggaggc gagcaactgg cccaccgggc cggccacgtc
     1321 ggcgtacagg ccggcgccgg cgatgatgcc gatgccgatc agggtgaggc cggccacgtc
     1381 cgccgagcgc ccgtcgagcg ccgcgccgac ggtgccgccg acccgcgacg gggccttggc
     1441 cggcttcttg gcggtgctgc gccgcttggc tggcggcttc ttcttctgac tcgagcgcgt
     1501 ggcagccaca gtggccacaa cggtaacagc agccacatct gccccgaggg agggtcgtcc
     1561 agcccgttct cggtgcgtgg cgttgcgcca gcgcaacgta atgcgcccag aacgcgccgg
     1621 ggcccggtcc ccctgcggac cgggccccga gatgtcggtt ggtgggacgg cgctcagccc
     1681 cgggtgatca ccacggtgca gtctcggggc acgctgccgc cgccgtcggg gaagctggac
     1741 gtcccggggt cgccgttccc ggggtgctgg atgttgacga acaggtcgtc cttgcctgcc
     1801 ggcgtgatgc cggtgacctc gcagccgggc acgccggtga ggaaccgacg gatctcgccg
     1861 ttgcgagggt cggccaccag catctggtcg ttggcgccct cgggctgtgc accgtcggtc
     1921 tggatgtaca tgcgcccgct ctcgtcgatc cacaggccgt cgggcgagcc gaacgcgtcc
     1981 tcgggatcga tcgtggcccc ggagccggcg ggacccgcga gcaggaagat gtcccagtcg
     2041 aacctggacg cggtgtgcga ggggtcgacg aacttgatga tgtgccccca ccggttctgg
     2101 gtccgggggt tggctgcatc agcggaggta cggccggtgt tgttcgtgca ggtgaagtac
     2161 acgtcgccgt tcaccgggtt ggcggccacc cactcgggcc gatccatctt cgtggcgccg
     2221 agggcgtcgg ccgcgagccg gacgttgacg accagctccg ccatgtcggc gaacggtgcg
     2281 ttgccgtcgt cggtccgggc tgcgttgacc ggatcgagct cgagcgggag ccagcggccg
     2341 gtgccgtcgg catcgaaccg ggcgacgctg agggtgccgt cgctgagcgg gctgacgccg
     2401 gcactgcgca tgtggcgcca tggccgtgcg gagacgtacc ggtacacgta gtcgtccgcc
     2461 tggtcgtcgc ccatgtaggc gacgacgcga ccgtcgggcg cctcgaccag ctccacgttc
     2521 tcgtgcttga agcgcccgag cgcggtgtgc ttctggatcg gcgagctcgg tgccatcggg
     2581 tcgatctcga cgatccaccc gtaccggttg gcctcgttga cgtgggcggg gtcgcgcagg
     2641 tcgaagcgct ggtcgtgcag gtgccaccgg tagccgaaac cggtggcggt gagcccgtag
     2701 cgctcctcgg actccgtgcg cgcgcccagc ggcttctcgg tgccgaagta gccgttgaag
     2761 ttctcctcgc aggtcagata ggtgccccac ggcgtcttgc cgttgccgca gttgttcagc
     2821 gtgccggcgg cctcgttgtc tgcagggttc tccagcagcg ccgaaccggc cgcgggaccc
     2881 gtgaacaccg cagcgccctt ggtgcccgtc acgcgccggt tcttcggacc catgacgacc
     2941 cgccactcgt tgcgcacccg ctcgatctcg acgacggaga tgccgtggac cgcctgggag
     3001 atggcgacca tctccggcgt gccgctctgg atgccggtgc cgttggtgat gtgcgggtcg
     3061 gtgccgtact cgtggttgat gcagagcagg ccgcgctcac cgtgcttgcc ggtcgggaag
     3121 aaccacatgc cgtcgtggcc gaggccgatg cgcttctcct ggtcctcggc gtcggtcggg
     3181 aaggtgccgc tccccgctcc ggggaggatc ggggtccccc acggcaggaa cgccgtggcc
     3241 gtgtacccct tcggcaccac gatctcgtcg ccgaagccga gcggcaccgg gcggaaggcc
     3301 agcagctccc ggttggctgc agacgcacca ccaccaggcg cgctggacgg gcgagcggcg
     3361 ccggcgatgc ccgtgccgaa gaaggagaag gcagcggcag ccacgccacc accgaggacg
     3421 gatcggcggg acaggcgcgc atcgagcacg tcctggaact gcgggttgtc ggtcgggttc
     3481 gtgatctcgg aatcgagcac gcggagcacc tcggggtcgt ttcgaacggg tcgctcgaaa
     3541 cgtagggagg cgaggtgacc gggcgatgtg gtccgggtga ccggcgggtc atccgcaggc
     3601 gaacactccg gggcgtccga gtgccgccgg ctcgatgtgc tgaggctcag gcctcgagga
     3661 ccaccgggac gatcatcggc ttgcgcttgg tctcgcggtt cacgaaccgg ccggcggcgc
     3721 ggcgggcagc ccgctggacc gcctcgatgt cggtggcgtc cttgacctcg gccacggcct
     3781 tctcgacctc gttggcgcac tcggtgagca ggccctccgc ctccttctcg tagatccacc
     3841 cgcgggtgac gatctcgggc cggttgagca ccgcgccggt ctcgacgtcg acggtgacga
     3901 tgacgacgac cacgccctcc tcggccagca ccttgcggtc gcggagcacg ccctggccga
     3961 cgtcaccgat ggtgccgtcg acgtacaggt agccagcggg caccttgccg acggcgcgca
     4021 ggccgtcgtc ggtgagctcg agctggtggc cgtcctcgca gacccacacg tggtcctggg
     4081 cggtgcccat cgcccgggcg atcttggcgt gggccacgag gtggcggtac tcaccgtgga
     4141 ccgggatgaa ccactcgggc ttgacgatcg agtggtacgt cttgagctcc tcggccttgg
     4201 cgtggccggt ggcgtgcacg tcggcgatgc cggagtacac gacctcgacg ccctggcgga
     4261 tcaacccgtc gatcaccttg gacacgttca tctcgttgcc ggggatcggg tgcgaggaca
     4321 ggatcacggt gtcatcgggc gtgagcgtga gccagcggct ctcggtggcg gccatgagcg
     4381 ccagcgccga catcggctcg ccctggctgc ccgtggagat gatgcagacc ttgcccgggt
     4441 cgtgcttgtc gatctgctcg atgtcgatga ggctgctctc ggggatgttc aggaggccga
     4501 gcgagcgggc cagcttcacg ttcttcttca tcgacatgcc gagggtggcg atcttgcggt
     4561 cgaaggcgac ggccgcgtcg gcgatctgct ggacgcggtg cacgtggctg gcgaaacagg
     4621 ccgtgatgat gcggcggccc tcgtgggcgt ggaacaggtc gtagaggacc cggccgacgc
     4681 tggtctcgct ggccgagtgg ccacgctcct ccgcgttggt cgagtcggcc agcagcaggc
     4741 ggatgcccgg atcatgcgcc atcgcgccga tgcgggtcag gtcggtgagg cgcccgtcga
     4801 ccggggtcag gtcgatcttg aagtcacccg agtgcaggat cgtgccctgc ggggtgtgga
     4861 acgcggtggc gaagccgtgg ggcaccgagt gggtggcggg gatgaactcc aggtcgaacg
     4921 ggccgacctt gcgccgctcc ccgtccttga cgatgttgaa gctggtgcga tcgaggagac
     4981 cggcctcctc gatgcggttc ttggcgaagc cgagggtgag ctcggagccg tacagcggga
     5041 agctgatgtc acgcagcagg aacgacaggc cgccggtgtg gtcctcgtgg ccgtgggtga
     5101 gcacggcgcc ctcgacccgg tcggcgttgt cgtagaggta gctgaagtcg ggaagcacca
     5161 ggtcgacgcc gagcatgtcc aggtccggga acatgagtcc gcagtcgatg agcatgatcc
     5221 ggccgtcgac ctcgatgcag gcgcagttgc gcccgatctc gccgaggccg cccaggaacg
     5281 tgatcttcac gggtgcaggc atggacctgc accgtactgg ccgggttcag gtacccagcg
     5341 agtcccagag ctccttggcc tggacctcga ggccgtcggg ggtgggtccc atgggcgggc
     5401 ggcactcccc ggccggcagg ccgagggtgc gcagcatcgc cttggtgggc accggattgg
     5461 gggcgtcgag ggtggactcg aacgcgtacg acgggagcag ggtctggttg atgcgggttg
     5521 cctcggcgac gtcgccggcg tcgaaggcgt cgaacatcgc ccggatctgg tcgcccaccc
     5581 agtgggaggc gacgctgatc gccccgcgcg ccccgacgga gagcagcggc agggtcaggt
     5641 tgtcgtcgcc ggagtagacg tcgaagtcct cgggggcgtg cgcgatgacg ccggcggtct
     5701 ccgccgggct cccggcggcg tccttcaggc cgacgatggt gggcacctcg tgggccaggg
     5761 tgaggatcgt gtcgctgtcg accttgcggc tggtccggcc ggggatgtcg tagaggacga
     5821 tcggcaggtc ggtcgcctcg gcgatcatgc ggaagtggcg gagcaggccg gcctgggacg
     5881 gcttgttgga gtacggcgcc accaccagca ggccgtcgac gccgatgccg gcagccgcct
     5941 cggactggcg gatggcgtgg gcggtgtcgt tggagccggt gccggcgatg accggcacgt
     6001 cgaccgcctg gcgcacggcc cggaacaggt cgacggactc ggtggccgag agcgtgggcg
     6061 actcgccggt ggtgccgttg acgaccaggc cgtcgct
//
LOCUS       DATISU010000176        13103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_380778, whole genome shotgun sequence.
ACCESSION   DATISU010000176 DATISU010000000
VERSION     DATISU010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 13103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13103
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380778"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VLR27_07270"
     CDS             complement(<1..277)
                     /locus_tag="VLR27_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03282.1"
                     /translation="MTGSASYARCADSAAAARERLDRAAVRLASLEQAQRDAVGALLE
                     LWPTSAWIAAGASSAKAWLLAYTGVSDREAFRLEQSAGLCHSLPELAA"
     gene            complement(328..786)
                     /locus_tag="VLR27_07275"
     CDS             complement(328..786)
                     /locus_tag="VLR27_07275"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HSP03283.1"
                     /translation="MTGARRPRNAMDRLRPIRRALRGLEAAQVRRFGRSVLSTLFRTP
                     VLVLETTGRRSGQQRQTALAFHRRPDGDLVVVGGAGGQRRTPDWVANVRARPDVHVIV
                     DRVRQPMTAVEMLGDERASAWDEVREVWPQIERYERRAGRPVPVFLLRSG"
     gene            890..1462
                     /locus_tag="VLR27_07280"
     CDS             890..1462
                     /locus_tag="VLR27_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03284.1"
                     /translation="MERRRPGHGRYAHPEREQRWILADLPDGATAWAEITDRYLDGTN
                     LRIRRVEGADGVVHKLTQKVRPVPDDPSRVMLTTMYVPDHEVTALTSLPGRELRKTRR
                     RIELDGRHVAVDVLHDRFEGLILAETELGIHEERMPMPPFACCDVTDDDRFAGGSLAT
                     MTDEALAELLAEVDELVASRSRAAGSSPSR"
     gene            complement(1422..1838)
                     /locus_tag="VLR27_07285"
     CDS             complement(1422..1838)
                     /locus_tag="VLR27_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03285.1"
                     /translation="MDEEAQGRRRRGLILAGIVLVVGVPLTVTTWLSERNVDRQASEM
                     AEDLRRAGRQVDDVSTLAGDELTSYWNDSGNPLADALGHGDAFRGAAFGTTGISLAYE
                     TSWGFGRRCVHLLLRDDAPVLTEITDSASCQPLAID"
     gene            1892..2503
                     /locus_tag="VLR27_07290"
     CDS             1892..2503
                     /locus_tag="VLR27_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HSP03286.1"
                     /translation="MHPSLRERAALCDLFEQLGPDAPTRCEGWRTTELAAHLHTREHR
                     LDAGPGLLGRGPFARYTAKLEAKAADRPYDELVAELRDGPPRQWFGRYVPAGDIHEWF
                     VHHEDVRRANGELPRLDDDLDEALWAALARWGKILTRKADVGVELVAHDGRSRQVKDG
                     DESVILRGRPGEIMLALFGRESASTLEGSETAVAAYHASTIGL"
     gene            2522..2884
                     /locus_tag="VLR27_07295"
     CDS             2522..2884
                     /locus_tag="VLR27_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HSP03287.1"
                     /translation="MAKNVLGTDLQSCSTDPMTGFYRDGCCSTGGDDAGVHTVCAVVT
                     DDFLEFSKAAGNDLSTPMPQFGFPGLRDGDQWCLCAPRWQEAFEAGKAPQVVLEATHV
                     QTLEWCTLSDLQAHAVRR"
     gene            complement(2912..5218)
                     /locus_tag="VLR27_07300"
     CDS             complement(2912..5218)
                     /locus_tag="VLR27_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSP03288.1"
                     /translation="MTVTGTDTEQPAAGGSILGTRVQRIEDPALLRGRGTYIDNVAPT
                     DALHVAFVRAFTAHGTIESIDTSDAEAMPGVVGVFTGADLDIGPLTSSPLLAHTVSQP
                     VLAVDRVRYVGEPIAVVVAETKAQAVDAAEAVWADVEPIEPILDPEAAARDEVVLHPD
                     LGTNVVLDQGDPVGPEQLEGDGVVVVSERLINQRMAAVPLEGRVVAARWEGDRLTQWS
                     CTQGAHGTRDELATLFGLDPSQVHVITPDVGGGFGAKHGTTPEELVVTWLARRLDRSV
                     RWAETRSENLVGMVQGRGQVQHASIAGTRDGTITGYRLDVIQEGGAYAGSGCVLPYMT
                     RMMAPGCYAVDDVRVRIRSVVTNTSTVGAYRGAGRPEAAAAIERMVDRFAAEIGMDPV
                     EVRRKNLLQPDAFPLRTATGGSYDSGDYPAVLDKALEAAGYGELRARQAELRSEGGPK
                     QLGIGVSAYVEITNVLRQSEYGSVEITADGGAIVRTGSSAHGQGHHTAWAMLVSDRTG
                     IPFDRIEVRHGDTDDIPRGGGTGGSKSLQIGGAAAAAAAELVVERAKQLAADLLEADP
                     SDIVHDQSAGTFAVAGTPVTAKSWADLAAAAPGQGMAVLAAETDFEPGGSTFPFGAHV
                     AVVEVDTDTGKVRLLRHIACDDAGTIVNPLLVDGQVHGGVAAGIGQALFEEVRYDDEG
                     NPLTTTFADYAFPSAAEMPSFERIPHETPTPHNPLGAKGIGESGTIGATPAVQNAVVD
                     AVAHLGVRHIDMPLTAERVWTAIRDASS"
     gene            complement(5343..7016)
                     /gene="ettA"
                     /locus_tag="VLR27_07305"
     CDS             complement(5343..7016)
                     /gene="ettA"
                     /locus_tag="VLR27_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HSP03289.1"
                     /translation="MAEFIYTMHKVSRFYPPDKEILKDISLSFFPGVKIGVIGHNGSG
                     KSSLLKIMAGLDDEYSGQARLSPGYTVGFLSQEPQLDESKDVRGNIMDGVANVAALQE
                     EYDALLASYADPDADYEKIGARQAELEAKMAASGVDDLDRTIDIAMDALRTPPGDADV
                     KVLSGGERRRVALCKLLLSKPDLLLLDEPTNHLDAESVAWLERTLKDYPGTVVAVTHD
                     RYFLDNVAGWILELDRGRGYPFEGNYSGWLEQKQARLEQEDRTDQKKRETLQRELEWI
                     RMSPKAKQSKGRARINSYEELRAQADQGSGRLEKLEIAIPPGDRLGDQVIDFESVAKG
                     YDDRLLIEDLSFKLPPAGIVGVIGPNGAGKTTMFRLITGAEEPDDGDIVIGKTVNLSY
                     VDQNRDVLDDERTVYEEITGGVDVLQIGRREINGRAYVASFNFKGSDQQKKVGVLSGG
                     ERNRVLLAKKLKEGGNVILLDEPTNDLDVDTLRALEDALETFPGCAVVISHDRWFLDR
                     LATHILAFEGDSQVRWFEGNFTEYEEWRRKEFGADATEPHRLKYKPITR"
     gene            7088..8218
                     /locus_tag="VLR27_07310"
     CDS             7088..8218
                     /locus_tag="VLR27_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03290.1"
                     /translation="MARCRVTYREAADGRPVRSHAGDLLELEVDAPPARPSWVGEVGA
                     APVRTPPAPRRRRPRWPWGAGLAALLVAVVAVELASTDDGGPEAAPTPSTTHTVAQEP
                     GPVSIATASVLSEPWGGVFEHHPDSREMVSVPRVHLASMAGDDARVLLVVDATRRAFG
                     GAEHSSPSGRTRVLGAPDGRGLRTLQWDEGGYGLSLMSVGLTADAQRAVAGTVHLPPG
                     PSLQHGRAPWFDDAALADLGLTVTERRSGTATAYGSPMIGQSGGGSVEGQLHRSGADP
                     VLVSVVQDQLVTAARVRRILGEAADVGATELPGVTGAAALDHGTAPETGRRIRGWARL
                     LLDHEVGVTIELSSDVLGPDELAEVAASMDLGRLARTASPIG"
     gene            complement(8215..8445)
                     /locus_tag="VLR27_07315"
     CDS             complement(8215..8445)
                     /locus_tag="VLR27_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03291.1"
                     /translation="MADTATTPGSLGAPVSQLKSLTDQLVTVTTDHGEVTGTVLSCTR
                     HSVWLVAGDDADVVVSLDEITDLVHHPASAAA"
     gene            8589..9071
                     /locus_tag="VLR27_07320"
     CDS             8589..9071
                     /locus_tag="VLR27_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03292.1"
                     /translation="MSVSTLIQPSDRRTVQATAGPAMAEWLLALGPVVHRLDYGGRVA
                     AVGGDGAAVALLAAAHPLASLDAVDAGTDALAAGSYDLVCALDGLAELADPVGTARRA
                     LRLVRPTGALVVVEASRPDGFVDGPITIGGWLLRAGAARVRLLAATSHGFVLDARTTH
                     "
     gene            9103..9471
                     /locus_tag="VLR27_07325"
     CDS             9103..9471
                     /locus_tag="VLR27_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HSP03293.1"
                     /translation="MEQIPLFERLGGRDGIRKMAERLLDNHLQNPLIATRYANATKDR
                     EQLVDGATEFFCTGLSGVPTYEGRPIADVHTGMNIDDAEFVAVLDDAIAAMGSLGVGE
                     PEQAEVLHILYSMKGDVVRL"
     gene            9500..12646
                     /locus_tag="VLR27_07330"
     CDS             9500..12646
                     /locus_tag="VLR27_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSP03294.1"
                     /translation="MAAADGSVSEAAVRIDVLGCFRVATDVSLVEEGDWPSRRSAELV
                     QLLALADRRQLLRDEVCDALWPHLSPDAAANNLRKAAHHARQVLGTADAVVLRGGTVA
                     LFPDREVLVDVDAFEVTGAAALAAADPEGCAAVADSCGGELLPVARYEDWAEPRRRSV
                     RALRLDLLRAAGRWADVVALEPTDEPAHQALMEAALRRGSRAEALRWYAALRRSLASE
                     LGVAPDDRSRTLHERCLASGSRSDAVACIGRDAELAVLEGAMRAAADRRGGAVVVHGG
                     AGSGKTTICRELGELATEAGWRCFWVTADGGDQPYGPLLPLLEELLVDDRDVLSRLAG
                     NAAAVLGALVPGLGGRPTEGPLSRHQVVGALQQLLRAVAGGPAVLVVDDAHLADEATI
                     DVVGHVAASVRDVVVVLARRDAPANASLAKVLRRLDRAGRSQELELRPLGDREADELL
                     RAVAPHLAESTRLRLREEAAGNAFVLVELARIAGQDADAVATVGAAMTARLADLAEAD
                     VDGLRRLALGGGDLDQSTALALFAGPEPELHDLLDTALDAGVLVVVGDRYRFAHELVR
                     SALAGQLPPHRRLEVHRRAARALADSGAAPAAVARHWLAGERPAEAVDPSLSAAERAV
                     ALGAFADARRHVEPVLAHDPANASALRIEAQSLDMMGDPRALRAYDAAIAAADPVAAH
                     DLMAMRALAQIKQGDPAGGLEAIAGASPTSVAGRLSEALTYAGAAALGFADPAFGTAK
                     AAESRRLALQTGDTASIVIASWAQAAAAHARGELHDSVLADLRDTAHVPHLAVRVFDG
                     HLCMTQRFLYGARPYDEVIAFADALADEALRLGAMRGHAFAMNLRGEAELHAGRIDEA
                     AADLEEGARLHRAIGGAVGEAHALQRLAEVAHHRGRSPEAAQILDEALDLARVTDIGF
                     HLLDRIYGTRVMLAGSDPEAALAAVDEAESAVQGQLETCPGCRITFAVPAAIACATAG
                     ELDRAARYEEASGWLAHVVMRLPAWDAAHEEVRGHVRLAAGDATAARQLFVRARAGFE
                     AAGHPLDAERCRRLVGAG"
     gene            12698..>13103
                     /gene="kgd"
                     /locus_tag="VLR27_07335"
     CDS             12698..>13103
                     /gene="kgd"
                     /locus_tag="VLR27_07335"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441890.1"
                     /note="kgd; produces succinic semialdehyde; part of
                     alternative pathway from alpha-ketoglutarate to succinate;
                     essential for normal growth; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HSP03295.1"
                     /translation="MAEKQTPDLVGPNAWMVDEMYEQYLADPSSVSESWQDFFADYRS
                     DVDAGSVSAPAANGADAPVAATPSTSPAPKADPPKKAEASEGEAGEPIRGVGARIVEN
                     LEKSLAVPTATSYRNVPAKLLEVNRAVINGYLG"
BASE COUNT         1713 a         4701 c         4888 g         1801 t
ORIGIN      
        1 ccgcagcaag ttccgggagc gaatggcaca gcccggcgct ctgctccagg cgaaaagcct
       61 cacggtcgga cacgccggtg taggcaagga gccacgcctt ggccgacgac gcaccggcgg
      121 cgatccacgc cgaggtcggc cacagctcga gcagggcgcc gaccgcatca cgctgtgcct
      181 gctccagcga tgcgagccga acggctgctc gatccagccg ctcccgcgcc gcagcggcgc
      241 tgtcggcgca acgtgcgtag gaggcggaac cggtcatggg acaaatcgta cgaagggggt
      301 ctgacaggaa ctccgcgtcc ccagcaacta cccggaccga agcaggaaga cggggaccgg
      361 gcgtcccgca cgtcgctcgt accgctcgat ctgcggccag acctcacgga cctcgtccca
      421 ggccgacgca cgctcgtcgc ccagcatctc gacggcggtc atcggctggc gcacccggtc
      481 gacgatgacg tggacgtccg gacgggcgcg gacgttggcg acccagtccg gcgtgcgacg
      541 ttgcccgccg gcgccgccga cgaccacgag gtcaccgtcg ggacggcggt ggaaggcgag
      601 cgcggtctgt cgctgctgac cgctcctgcg cccggtcgtc tccagcacca gcaccggcgt
      661 gcggaacagc gtcgacagga cgctccggcc gaagcggcgg acctgcgctg cttcgagccc
      721 tcgcagcgca cgacggatcg gccggagccg gtccatggca ttgcggggcc tgcgcgcacc
      781 ggtcacgggc gagaggttgc cggagtgccg ctggccccgc cacccgtcac gacagaccag
      841 ggacctccgg ccctgccgaa gaccgtcgtc gccgtgggac agtgccggca tggagcgacg
      901 ccgacccggc catggccgct acgcccaccc cgagcgcgag cagcggtgga tcctcgccga
      961 cctgcccgac ggcgccacgg cgtgggcgga gatcaccgac cgctacctcg acggcaccaa
     1021 cctccggatc cgccgggtcg agggtgcgga cggcgtcgtg cacaagctca cgcagaaggt
     1081 gcgccccgtc cccgacgacc cgagccgggt gatgctcacg accatgtacg tgccggacca
     1141 cgaggtgacc gcgctgacgt cgctgccggg tcgggagctg cgcaagaccc gtcgccgcat
     1201 cgagctcgac ggccgacacg tcgcggtgga cgtcctccac gaccggttcg agggcctgat
     1261 cctggctgag accgagctcg ggatacacga ggagcggatg cccatgccgc cgttcgcgtg
     1321 ctgcgacgtg accgacgacg accgcttcgc cggcggttcc ctcgcgacga tgaccgacga
     1381 ggcgctggcc gagctcctcg ccgaggtcga cgagctggtc gctagtcgat cgcgagcggc
     1441 tggcagctcg ccgagtcggt gatctcggtc agcaccgggg cgtcgtcgcg cagcagcagg
     1501 tggacgcatc gacggccgaa gccccagctg gtctcgtagg cgaggctgat gccagtggtg
     1561 ccgaaggcgg cgccgcggaa cgcgtctccg tgcccgagcg cgtcggcgag agggttgccg
     1621 gaatcgttcc agtacgacgt cagctcgtcg ccggcgaggg tggagacgtc gtccacctgg
     1681 cgaccggcgc gtcgcaggtc ctcggccatc tcgctggcct ggcgatcgac gttgcgctcc
     1741 gacagccagg tcgtgacggt caggggcacc cccacgacga ggacgatgcc ggcgaggatc
     1801 agcccccgcc gccgccgtcc ctgtgcctcc tcgtccacga cgccacggta ggcgcggcgg
     1861 ccggcgcccc gaccggcggt agacagggcc gatgcacccc tccctccgcg agcgcgccgc
     1921 cctctgcgac ctgttcgagc agctcggccc cgacgcgccc acccggtgcg agggatggcg
     1981 gacgaccgag ctcgccgccc acctgcacac ccgcgagcac cgcctcgacg ccggccccgg
     2041 actgctcggc cggggtccct tcgcccgcta caccgccaag ctcgaggcca aggcggcgga
     2101 ccgtccctac gacgagctgg tcgccgagct gcgcgacggc ccgccccggc agtggttcgg
     2161 gcgctatgtg ccggcgggcg acatccacga gtggttcgtg caccatgaag acgtccgccg
     2221 ggccaacggc gagctgccgc ggctcgacga cgacctcgac gaggcgctct gggccgccct
     2281 ggcgcgctgg ggcaagatcc tcacccgcaa ggccgacgtc ggcgtcgagc tggtggccca
     2341 cgacgggcgc agccggcagg tcaaggacgg cgacgagtcg gtgatcctgc ggggacgccc
     2401 gggcgagatc atgctcgccc tcttcggccg ggagtccgcc agcaccctcg agggcagcga
     2461 gaccgcggtc gcggcgtacc acgcgtccac catcgggctc tgagcggtcc acactggggc
     2521 catggccaag aacgtcctcg ggaccgacct gcagagctgc tccaccgacc cgatgaccgg
     2581 gttctaccga gacgggtgct gcagcaccgg tggtgacgac gccggtgtgc acacggtctg
     2641 cgcggtggtc acggacgact tcctcgagtt ctccaaggcc gccggcaacg acctgtcgac
     2701 gccgatgccc cagttcgggt tccccggcct gcgcgacggg gaccagtggt gcctctgtgc
     2761 tccgcgctgg caggaggcgt tcgaagccgg caaggcgccc caggtcgtgc tcgaggccac
     2821 ccacgtccag acgctcgagt ggtgcaccct cagcgacctg caggcccacg ccgtccggcg
     2881 ctgagcgcgc gctacaccac gactgccagt ttcaggaaga tgcgtcccgg atggcggtcc
     2941 agacccgttc ggcggtgagc ggcatgtcga tgtggcgcac gccgaggtgg gcgacggcgt
     3001 cgacgaccgc gttctgcacc gccggggtgg cgccgatggt cccggactca ccgatgccct
     3061 tggcgccgag cgggttgtgc ggcgtggggg tctcgtgcgg gatccgctcg aacgacggca
     3121 tctctgctgc ggacgggaac gcgtagtcgg cgaaggtcgt ggtgagcggg ttgccctcgt
     3181 cgtcgtagcg cacctcctcg aacagcgcct ggccgatgcc ggcggcgacg cccccgtgga
     3241 cctggccgtc gaccagcagc gggttcacga tggtgccggc gtcgtcgcag gcgatgtggc
     3301 gcagcaggcg gaccttgccg gtgtcggtgt cgacctcgac cacggcgacg tgcgcgccga
     3361 agggaaacgt ggagccaccg ggctcgaagt cggtctccgc cgccagcacc gccatgccct
     3421 ggcccggcgc cgcggcggcg aggtcggccc acgacttggc ggtgaccggg gtgccggcga
     3481 cggcgaaggt gccggcggac tggtcgtgca cgatgtcgga cggatcggcc tcgagcaggt
     3541 cggcagcgag ctgcttggcc cgctcgacca cgagctcggc ggcggcggcc gcggccgcgc
     3601 cgccgatctg cagcgacttg gacccgccgg tgccgccacc gcgggggatg tcgtcggtgt
     3661 cgccgtggcg gacctcgatg cggtcgaacg ggatgccggt gcggtccgac acgagcatcg
     3721 cccacgcggt gtggtggccc tggccgtgag cggaggagcc ggtgcggacg atggcaccac
     3781 cgtccgccgt gatctccacc gagccgtact cgctctggcg cagcacgttg gtgatctcga
     3841 cgtaggcgga cacgccgatg ccgagctgct tgggtccgcc ctcggagcgg agctcggcct
     3901 gacgggcgcg cagctcgccg tagccggccg cctccagcgc cttgtcgagc acggcggggt
     3961 agtcgccgct gtcgtagctg ccgccggtgg cggtgcggag cgggaaggcg tcgggctgga
     4021 ggaggttctt gcgtcgcacc tcgaccgggt ccatcccgat ctcggccgcg aagcggtcga
     4081 ccatgcgctc gatggcagca gcggcctcgg gccgtccggc gccgcggtag gcgccgacgg
     4141 tggaggtgtt ggtgacgacc gagcggatgc ggacgcgcac gtcgtcgacg gcgtagcaac
     4201 ccggtgccat catccgggtc atgtacggca ggacgcagcc gctgccggcg taggcgccgc
     4261 cctcctggat gacgtcgagg cggtagccgg tgatggtgcc gtcgcgggtg ccggcgatgc
     4321 tggcgtgctg cacctggccc cggccctgga ccatgccgac caggttctcc gagcgggtct
     4381 cggcccagcg cacggatcga tccaggcggc gagcgagcca ggtgaccacc agctcctcgg
     4441 gtgtggtgcc gtgcttggcg ccgaagccgc cgccgacgtc gggcgtgatg acgtgcacct
     4501 gggacggatc gagcccgaac agggtcgcca gctcgtcgcg ggtgccgtgg gcgccctgcg
     4561 tgcaggacca ctgggtgagc cggtcgccct cccaccgggc cgcgacgacg cggccctcga
     4621 gcggcaccgc cgccatgcgt tggttgatca gccgctcgga cacgacgacg acgccgtcgc
     4681 cctcgagctg ctcgggtccg accgggtccc cctggtcgag cacgacgttg gtgccgaggt
     4741 cggggtggag cacgacctcg tcgcgagcgg cggcctcggg gtcgaggatc ggctcgatcg
     4801 gttcgacgtc ggcccacacg gcctcggcgg cgtcgacggc ctgggccttg gtctcggcca
     4861 cgacgaccgc gatcggctcg ccgacgtagc ggacccggtc gacggcgagc acgggctggg
     4921 acaccgtgtg ggccaggagg gggctggagg tgagcggacc gatgtcgaga tcggcaccgg
     4981 tgaacacgcc gaccacgccg ggcatggcct cggcgtcgga cgtgtcgatg gactcgatgg
     5041 tgccgtgggc ggtgaaggcc cgcacgaacg ccacgtgcag cgcgtcggtg ggggcgacgt
     5101 tgtcgatgta ggtgccgcgc ccgcgcagca gcgccgggtc ctcgatgcgc tgcacgcgcg
     5161 tgccgaggat gctcccgccc gcggctggct gctccgtgtc cgtccccgtg accgtcatgg
     5221 ccgcggttct agtggagcgg cggaactctc gcgggccggt cacgccctcc cgcggttctg
     5281 atggcggctt cgccgccgga gagcccgtga catgggtcgc ctcgccttcg gctcggctcc
     5341 agctaccggg tgatcggctt gtacttgagc cggtggggtt cggtggcgtc ggcgccgaac
     5401 tccttgcggc gccactcctc gtactcggtg aagttgccct cgaaccagcg cacctggctg
     5461 tcgccctcga aggcgaggat gtgggtggcg aggcggtcga ggaaccagcg gtcgtgggag
     5521 atgaccacgg cgcagcccgg gaacgtctcg agcgcgtcct cgagggcgcg cagggtgtcg
     5581 acatcgaggt cgttggtggg ctcgtcgagg aggatgacgt tgccgccctc cttgagcttc
     5641 ttggccagca gcacccggtt gcgctcgccg ccggagagca cgccgacctt cttctgctgg
     5701 tcggagccct tgaagttgaa cgaggccacg taggcgcggc cgttgatctc gcggcgaccg
     5761 atctggagga cgtcgacgcc gccggtgatc tcctcgtaga ccgtgcgctc gtcgtcgagg
     5821 acgtcgcggt tctggtcgac gtaggacagg ttgaccgtct tgccgatgac gatgtcgccg
     5881 tcgtcgggct cctcggcgcc ggtgatgagc cggaacatcg tggtcttgcc ggcgccgttc
     5941 gggccgatga cgccgacgat gccggccggc ggcagcttga agctgaggtc ctcgatgagg
     6001 aggcggtcgt cgtagccctt ggccaccgac tcgaagtcga tgacctggtc gccgaggcga
     6061 tcgcccggcg ggatggcgat ctccagcttc tccagccggc ccgagccctg gtcggcctgg
     6121 gcgcgcagct cctcgtagga gttgatgcgg gcccggccct tggactgctt ggccttgggc
     6181 gacatccgga tccactcgag ctcgcgctgg agggtctcgc gcttcttctg gtcggtgcgg
     6241 tcctcctgct cgaggcgcgc ctgcttctgc tccagccagc ccgagtagtt gccctcgaac
     6301 gggtagcccc ggccgcggtc gagctccagg atccagccgg ccacgttgtc gaggaagtac
     6361 cggtcgtggg tgacggccac gacggtgccc gggtagtcct tcagcgtgcg ctccagccac
     6421 gccaccgact cggcgtcgag gtggttggtg ggctcgtcga gcaggagcag gtcgggcttg
     6481 gacagcagca gcttgcacag cgccacccgg cgacgctcgc cgccggagag gaccttgacg
     6541 tccgcgtcgc ccgggggcgt gcgcagggcg tccatggcga tgtcgatggt gcgatcgagg
     6601 tcgtccacgc cgctggcggc catcttggcc tcgagctcgg cctggcgggc gccgatcttc
     6661 tcgtagtcgg catcggggtc ggcgtaggac gcgagcagcg cgtcgtactc ctcctgcagc
     6721 gcggccacgt tggcgacgcc gtccatgatg ttgccccgga cgtccttgct ctcgtcgagc
     6781 tggggctcct gggacaggaa cccgacggtg tagccggggg agaggcgggc ctggcccgag
     6841 tactcgtcgt cgaggccggc catgatcttc agcagcgacg acttgcccga gccgttgtgc
     6901 ccgatgacgc cgatcttcac gcccgggaag aacgacaggg agatgtcctt caggatctcc
     6961 ttgtccggcg ggtagaagcg ggacaccttg tgcatggtgt agatgaactc ggccacgtgg
     7021 gcaccctatt ggccgctgtc ccgggcgccg tatccccgga tgcggcaccg ggctaccgtc
     7081 cacggtgatg gcgcggtgcc gggtgaccta ccgggaggcc gccgatgggc ggcccgtccg
     7141 gagccacgcg ggcgacctgc tggagctcga ggtggacgca cccccggccc ggccgtcctg
     7201 ggtcggcgag gtgggcgccg cgccggtccg caccccgccg gcgccacggc gacggcggcc
     7261 ccggtggccc tggggcgccg ggcttgccgc cctcctcgtc gccgtcgtgg ccgtggagct
     7321 ggcgagcacg gacgacggcg ggccggaggc agctccgacc ccgtcgacga cgcacaccgt
     7381 ggcccaggag ccgggcccgg tgtcgatcgc caccgcgtcg gtcctgtccg agccgtgggg
     7441 cggggtgttc gagcaccacc cggactcccg ggagatggtg tcggtgccgc gcgtccacct
     7501 cgcgtcgatg gccggcgacg acgcccgcgt gctgctggtg gtcgacgcga cccgccgggc
     7561 gttcggcggc gcggagcact cgtcgcccag cggtcggacc cgcgtcctcg gcgcgccgga
     7621 cgggcgaggg ctgcgcaccc tgcagtggga cgagggcggc tacgggctct cgctcatgag
     7681 cgtggggctc acggccgacg cccagcgggc cgttgccggg accgtgcacc tgccgcccgg
     7741 accgtcgctg cagcacgggc gtgccccgtg gttcgacgat gcggcgctcg ccgacctcgg
     7801 cctgacggtc accgagcggc gcagcgggac cgcgacggcc tacggcagcc cgatgatcgg
     7861 ccagtccggg ggcggctcgg tcgaggggca gctgcaccgc tccggtgccg acccggtgct
     7921 ggtcagcgtc gtccaggacc agctcgtcac cgctgcccgg gtgcgtcgga tcctggggga
     7981 ggctgcggac gtcggcgcca ccgagctgcc gggcgtcacg ggggctgccg ctctcgacca
     8041 cggcaccgcg ccggagaccg gtcgtcgcat ccggggttgg gcccgcctcc tgctcgacca
     8101 cgaggtcggg gtgacgatcg agctgtcgtc ggacgtgctc ggccccgacg agctcgccga
     8161 ggtggccgcg tcgatggacc tgggccggtt ggcccggacg gcgtcgccga tcggttaggc
     8221 ggcggcggac gccgggtggt ggacgaggtc ggtgatctcg tccagcgaga cgacgacgtc
     8281 ggcgtcgtcg ccggcgacga gccagaccga gtggcgcgtg cacgacagca cggtgccggt
     8341 cacctcgccg tggtcggtgg tgacggtcac gagctggtcg gtgagcgact tgagctgcga
     8401 gaccggcgca ccgagggacc cgggggtcgt tgcggtgtct gccatgcgca tggttctaca
     8461 gaagcgcgga cgtcgattgg tggttgccga cacatcgtga ccgactggaa acgcgccgga
     8521 cacgatcggg aacgttcgag gaacgcgacg ggcggatgct gctcccaccg acgaacgagg
     8581 gagcgcagat gtccgtgagc acgctgatcc aaccgagcga ccgccgaacg gtgcaggcca
     8641 ccgccggccc ggcgatggcc gagtggctgc tggccctcgg acccgtcgtg caccggctcg
     8701 actacggcgg ccgagtggcc gccgtcgggg gcgatggcgc cgcggtcgcc ctgctcgccg
     8761 ccgcccaccc gctcgcgagc ctcgatgcgg tcgacgccgg caccgacgcg ctcgccgcgg
     8821 ggtcctacga cctcgtctgc gcgctcgacg ggctcgccga gctggccgac cccgtgggca
     8881 cggcccggcg cgcgctgcga ctggtccggc cgaccggggc gctcgtcgtc gtggaggcct
     8941 ctcgcccgga cggcttcgtc gacggtccga tcacgatcgg cggctggctc ctccgggccg
     9001 gcgcggcgcg cgtacggctc ctcgccgcca cctcgcacgg gttcgtcctc gacgcccgca
     9061 cgacccactg atcccgaacc acccacccac aaggagcaca ccatggagca gatcccactc
     9121 ttcgagcgcc tcggcggtcg tgacggcatc cggaagatgg ccgagcgcct gctggacaac
     9181 cacctgcaga acccgctcat cgccacccgc tacgcgaacg cgaccaagga tcgtgagcag
     9241 ctggtggacg gagcgaccga gttcttctgc acgggcctgt ccggcgtgcc gacctacgag
     9301 gggcgtccga tcgccgacgt gcacaccggg atgaacatcg atgatgccga gttcgtcgcc
     9361 gtgctcgacg atgcgatcgc cgccatgggg tcgctcggcg tcggggagcc cgagcaggcg
     9421 gaggtcctcc acatcctcta ctccatgaag ggcgacgtcg tccggctctg atccggcagg
     9481 cgaggcgtag ggtggcggcg tggctgcagc cgacgggtcg gtcagcgaag cggcggtgcg
     9541 catcgatgtg ctcggctgct tccgcgtcgc caccgacgtc agcctcgtcg aggaggggga
     9601 ctggccgagc cgccgctcgg ccgagctggt gcagctgctc gcgctggcgg atcgtcgcca
     9661 gctcctccgt gacgaggtgt gcgacgcgct gtggccgcac ctgtcgcccg acgctgcggc
     9721 caacaacctg cggaaggcgg cgcaccacgc ccgccaggtg ctcggcacgg ccgacgcagt
     9781 cgtgctccga ggcgggaccg tcgcgctctt ccccgaccga gaggtgctgg tcgatgtcga
     9841 cgccttcgag gtcaccggcg ctgcggcgct cgctgccgcc gatccggagg ggtgcgccgc
     9901 cgtcgccgac tcgtgcggtg gtgagctcct ccccgtcgcc cgctacgagg actgggccga
     9961 gccccgtcgg cgcagcgtgc gggcgctgcg cctcgacctg ctccgcgccg caggtcggtg
    10021 ggccgatgtc gtggctctgg agcccaccga cgagccggcg caccaggcgc tgatggaggc
    10081 ggcgctccga cgtggctccc gcgccgaggc gctgcgctgg tacgccgccc tccgccgctc
    10141 gctggcctcc gagctcggcg tcgcacccga cgaccgctcc cggaccctcc acgagcgctg
    10201 cctggcgagc ggctcccgga gcgatgcggt ggcgtgcatc ggccgcgatg ccgagctcgc
    10261 cgtgctcgag ggcgcgatgc gggccgccgc cgaccggcgt gggggagccg tcgtcgtgca
    10321 cgggggcgcg ggatcgggga agacgacgat ctgccgggag ctcggggagc tcgccaccga
    10381 ggccggctgg cggtgcttct gggtcactgc cgacgggggc gaccagccct acgggcccct
    10441 gctgccgctg ctggaggagc tgctcgtcga cgaccgggac gtgctgtcgc ggctcgcagg
    10501 caacgcagca gcggtcctcg gcgcactggt ccccggcctc ggtggccgac cgaccgaggg
    10561 gccgctctct cggcaccagg tcgtcggggc cctgcagcag ctcctccggg cagttgccgg
    10621 cgggccggct gtcctggtgg tcgacgacgc tcacctggcc gacgaggcga cgatcgacgt
    10681 ggtggggcac gtcgcggcct cggtgcgcga cgtcgtcgtg gtgctcgctc gccgcgacgc
    10741 gcccgccaac gcgtcgctgg ccaaggtgct gcggcggctc gaccgggccg gtcggtccca
    10801 ggagctggag ctgcgaccgc tgggcgaccg cgaggccgac gagctgctcc gggcggtcgc
    10861 gccgcacctg gccgagtcga cccgcctccg gctgcgggag gaggcggcgg ggaacgcctt
    10921 cgtgctggtg gagctggccc gcatcgcggg ccaggacgca gacgcggtgg ccacggtcgg
    10981 cgccgcgatg acggcacgcc tggccgatct ggccgaggcc gacgtcgacg ggttgcggcg
    11041 gctggccctc ggcggcggcg acctggacca gtccacggcg ctcgccctgt tcgccgggcc
    11101 cgagccggag ctgcacgacc tcctcgacac tgccctcgat gccggcgtgc tcgtggtcgt
    11161 gggcgatcgc taccgcttcg cccacgagct ggtccgcagc gcactggccg gccagctgcc
    11221 cccgcaccgc cgcctcgagg tgcaccgtcg ggcggcgagg gcgctggccg acagcggtgc
    11281 cgcgccggcg gcggtggccc ggcactggct cgccggcgag cgccccgccg aggccgtgga
    11341 tccctcgctc tccgccgccg agcgtgcggt ggccctcggc gccttcgccg atgcgcgacg
    11401 ccacgtcgaa ccggtgctcg cccacgaccc ggcgaacgcc tcggcgctcc gcatcgaagc
    11461 ccagtccctc gacatgatgg gcgacccccg agcgctgcgc gcctacgacg ccgccatcgc
    11521 cgcagccgac cctgtggcgg cgcacgatct catggccatg cgggccctcg cccagatcaa
    11581 gcaaggagat cccgctggtg ggctggaggc gatcgccggc gccagcccga cgtcggtggc
    11641 cggccgcctg agcgaggccc tcacctacgc cggtgcggcc gcgctcggct tcgccgaccc
    11701 ggcgttcggc accgcgaagg cggcggagag ccggcgcctc gccctgcaga cgggggacac
    11761 ggcatcgatc gtgatcgcgt cgtgggcgca ggccgccgcc gcccacgccc gaggggagct
    11821 gcacgactcc gtgctcgccg acctgcggga caccgcgcac gtgccccacc tcgccgtgcg
    11881 ggtcttcgac ggccacctct gcatgaccca gcgcttcctc tacggcgccc gtccgtacga
    11941 cgaggtcatc gcgttcgcag acgcgctcgc cgacgaggcg ctgcggttgg gggcgatgcg
    12001 cggccacgcc ttcgccatga acctccgggg cgaggccgag ctgcacgccg ggcgcatcga
    12061 cgaggcggcg gccgacctcg aggagggggc gcggctgcac cgggccatcg ggggagcggt
    12121 cggcgaggcc cacgccctgc agcgcctggc cgaggtcgcc caccaccgcg gccggtcacc
    12181 cgaggcggcg cagatcctcg acgaggccct ggacctggct cgtgtgaccg acatcggctt
    12241 ccacctgctc gaccggatct acgggacccg ggtcatgctg gccggaagcg accccgaggc
    12301 cgcgctggca gccgtcgacg aggccgagag cgcggtgcag ggccagctcg agacgtgtcc
    12361 ggggtgccgg atcaccttcg ccgtgccggc cgcgatcgcc tgcgccacgg ccggggagct
    12421 cgaccgggcc gcccgctacg aggaggccag cggctggttg gcccacgtgg tgatgcggct
    12481 gcccgcctgg gacgcggccc acgaggaggt ccggggccac gtgcgcctcg ctgcgggtga
    12541 cgccaccgcg gcgaggcagc tgttcgtccg ggcccgggcc ggcttcgaag cggcaggcca
    12601 ccccctcgac gcagagcggt gccgccggct cgtcggggcc ggatgatcag cgatcgcgag
    12661 caacggcgcc gcccggggcc ggcggtacgc tcgccaggtg gctgagaagc agaccccgga
    12721 cctcgtcgga cccaacgcat ggatggtcga cgagatgtac gagcagtacc tcgccgaccc
    12781 ctcatcggtg agcgagtcct ggcaggactt cttcgccgac taccggagcg acgtcgacgc
    12841 cggctccgtc tcggcacctg cggccaacgg cgccgatgcg ccggtcgcgg ccactccttc
    12901 gacgtcgcct gctccgaagg ccgacccgcc gaagaaggcc gaggccagcg agggcgaggc
    12961 cggcgagccg atccgcggcg tgggggcccg catcgtcgag aacctggaga agagcctcgc
    13021 cgtcccgacg gccaccagct accgcaacgt cccggcgaag ctgctcgagg tcaaccgcgc
    13081 cgtcatcaac ggctacctcg gtc
//
LOCUS       DATISU010000177         1923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_474049, whole genome shotgun sequence.
ACCESSION   DATISU010000177 DATISU010000000
VERSION     DATISU010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474049"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VLR27_07340"
     CDS             complement(<1..197)
                     /locus_tag="VLR27_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03296.1"
                     /translation="MTASPVTGPIPGDDPEKRFVEGDDVLLIDRKRRRYLVTLTSGKE
                     FHSHAGVLAHDQLLGSVEGTT"
     gene            complement(194..1150)
                     /locus_tag="VLR27_07345"
     CDS             complement(194..1150)
                     /locus_tag="VLR27_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP03297.1"
                     /translation="MKCRTCRGPAIIDLKAHNANFCAEHFLAFCREQVARAIKKHRML
                     GPDERVLVAVSGGKDSLAVWDILLELGYQADGFVIGLGIGEYSETSTAYARAFAEERG
                     LTLHETSLRDDHGFDVPTAAEVTRRVPCSACGLSKRHLFDKAAIDGGYDAVATGHNLD
                     DEAAVLFGNTLRWNTEYLGRQRPVLPARDGFPRKIKPLVRLGEREMAAYCVIRGIDYI
                     VEECPMAAGNKHLRYKEALNAIEAESPGAKHAFYHEFLAKAADRFADDAADAVAAGTS
                     VLGTCTRCGSPASNEICAFCLLVERTADAVPVTLGQRRGAQR"
     gene            complement(1153..1350)
                     /locus_tag="VLR27_07350"
     CDS             complement(1153..1350)
                     /locus_tag="VLR27_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSP03298.1"
                     /translation="MKVLLRNPRRELDVDGPVRVDTLLRRLEINRESVLVIRSGELVP
                     GDAELGDDDVIEIRPVISGGA"
     gene            complement(1405..1671)
                     /locus_tag="VLR27_07355"
     CDS             complement(1405..1671)
                     /locus_tag="VLR27_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03299.1"
                     /translation="MAVPRFAKVAAWAELRARTDGLRGSSSAWMAIWVLLTGYRYIKR
                     KAAPDPVVIRERLEPGQQLVVTHFAKGAAPDDPVASRRRRRRRS"
     gene            complement(1675..>1923)
                     /locus_tag="VLR27_07360"
     CDS             complement(1675..>1923)
                     /locus_tag="VLR27_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03300.1"
                     /translation="ARLGRRPHHAREEVVATDKITRDDIEAKLRELKEDVDDTTESAK
                     SYAAAAAAGVVVLVFLVAFAAGKKRGRRKSTVVEIRRI"
BASE COUNT          261 a          644 c          726 g          292 t
ORIGIN      
        1 tacgtcgtgc cctccaccga cccgagcagc tggtcgtggg cgaggacgcc ggcgtggctg
       61 tggaactcct tgcccgacgt gagcgtgacc aggtagcggc gccgcttgcg gtcgatgagc
      121 aggacgtcgt cgccctcgac gaagcgcttc tcggggtcgt cgccggggat gggtccggtg
      181 accggtgagg cggtcatcgc tgcgcgcccc ggcgctggcc gagggtcacc ggcacggcat
      241 cggcggtgcg ctcgaccagc aggcagaagg cgcagatctc gttggacgcc ggcgacccgc
      301 agcgggtgca ggtgccgagg accgacgtgc cggcggcgac cgcgtcggcc gcatcgtcgg
      361 cgaagcggtc ggcggccttg gccaggaact cgtggtagaa ggcgtgcttc gcaccgggcg
      421 actcggcctc gatggcgttc aaggcctcct tgtaccgcag gtgcttgttt ccggcggcca
      481 tggggcactc ctcgacgatg tagtcgatgc cgcggatgac gcagtacgcg gccatctcac
      541 gttcaccgag gcggacgagc ggcttgatct tgcgggggaa gccgtcgcgt gcgggcagga
      601 cgggccgctg gcggcccagg tactcggtgt tccagcgcag ggtgttgccg aacagcacgg
      661 cggcctcgtc gtcgaggttg tggccggtgg cgacggcgtc gtagccgccg tcgatcgcgg
      721 ccttgtcgaa gaggtggcgc ttgctcaggc cacacgccga gcacggcacc cggcgggtca
      781 cctcggccgc ggtggggacg tcgaagccgt ggtcgtcgcg cagggacgtc tcgtggaggg
      841 tgaggccccg ctcctcggcg aacgcccggg cgtaggcggt ggaggtctcc gagtactcgc
      901 cgatgccgag gccgatgacg aagccgtctg cctggtaccc gagctcgagg aggatgtccc
      961 acacggccag ggagtccttg ccgccggaca ccgcgaccag cacccgttcg tcggggccga
     1021 gcatgcggtg cttcttgatg gcccgggcca cctgctcgcg gcagaaggcg aggaagtgct
     1081 cggcgcagaa gttggcgttg tgggccttca ggtcgatgat cgccgggccc cggcaggtgc
     1141 ggcacttcac ggtcacgcac ctccggagat gaccgggcgg atctcgatca cgtcgtcgtc
     1201 gccgagctcg gcgtcgccgg gcaccagctc accgctgcgg atgaccaaca ccgactcgcg
     1261 gttgatctcg aggcgacgca gcagcgtgtc gacccgcacg gggccgtcca cgtcgagctc
     1321 gcgccggggg ttgcgcagga gcaccttcat gcaccgacca tcttggctgc tggccgggct
     1381 gccgcggcag tccgcagccg gtggtcagct gcggcgacgg cggcggcgcc ggctcgccac
     1441 cgggtcgtcc ggcgctgcgc ccttcgcgaa gtgggtcacg accagctgct ggccgggctc
     1501 gaggcgctcg cggatgacga ccggatcggg cgcggccttg cgcttgatgt agcggtagcc
     1561 ggtgagcagg acccagatcg ccatccaggc ggacgacgac ccgcggagcc cgtcggtgcg
     1621 ggcccgcagc tcggcccagg cggcgacctt ggcgaaccgg gggacggcca cccgctagat
     1681 ccgtcggatc tcgacgacgg tcgacttgcg ccggccgcgc ttcttgccgg cggcgaaggc
     1741 caccaggaag acgaggacca cgacgccggc ggccgccgca gccgcgtagg acttggccga
     1801 ctcggtcgtg tcgtcgacgt cctccttcag ctcacggagc ttggcctcga tgtcgtcgcg
     1861 ggtgatcttg tcggtggcca ctacttcttc cctcgcatga tgagggcgcc gaccgagccg
     1921 agc
//
LOCUS       DATISU010000178         5478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_520738, whole genome shotgun sequence.
ACCESSION   DATISU010000178 DATISU010000000
VERSION     DATISU010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520738"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..869
                     /locus_tag="VLR27_07365"
                     /pseudo
     CDS             54..869
                     /locus_tag="VLR27_07365"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
     gene            866..1750
                     /locus_tag="VLR27_07370"
     CDS             866..1750
                     /locus_tag="VLR27_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HSP03301.1"
                     /translation="MSATEATLRPRRSVLYMPGANERALEKAKGLTADALILDLEDAV
                     APDAKAEARDRVCAAAASGEYGMREIAIRANGLDTQWHADDVAAIAEAGPDAIVIPKI
                     NSVEDVRAIESALDSAGAPDRTKIWAMVETPIAMLHAEEIAGCSERLSVLVMGTNDLA
                     KELHAQHVPGRQPLLTGLGLCLLAARATGKVILDGVYNDIKDEAGFLAECRQGKEMGF
                     DGKTLIHPSQLDPCNEVFAPSEDEVASAREIIAAFEEAEREGRGVVTVNGRMIENLHV
                     DQARQTLAQAEAIAALES"
     gene            complement(1771..2955)
                     /locus_tag="VLR27_07375"
     CDS             complement(1771..2955)
                     /locus_tag="VLR27_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HSP03302.1"
                     /translation="MTGPPGAAEERLARIAEAQRLLASVSHRLGPSLELETTLKAVLA
                     AMRELVDFRGGSVSLVEHGTVGIAASDPPVSPEVLDVRLPVGSGIGGRVVATGETIYV
                     PDLDADDRVDPEVRRAGSNAGMVSFLAVPLVCLGRPIGLLQVDSEVADAFDDVDIMLL
                     EGLAAQTATAIESARQSHERDRVDALKHGFINLVSHELRTPLTIAAGMLHMHRNLHPD
                     LPDADRLLDRADAALGRLGRLIEELIVMSELGAGEVRAVHRPVRVRQLIEEVVDASAD
                     PSLVTVRCDPGLEWTTDDRLVRIALGALVENALLYAGGAEVVAHAGVLEVCDHGPGLP
                     DDVLDRRGEAFSRSVRNPTTVAGLGLGLSMVTAMVAELDGDLSVETAPGEGTTIRIHL
                     PV"
     gene            3019..3738
                     /locus_tag="VLR27_07380"
     CDS             3019..3738
                     /locus_tag="VLR27_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HSP03303.1"
                     /translation="MAAPTTPTAVDFHFDVMCPFAYQTSRWIRAVRDQTDLDITWRFF
                     SLEEINRREGKKHPWERDWSYGWSMMRIGALLRRRDMAELDAWYERAGRALHEDGHKP
                     HDPDVARHLLTEIGLDPGLVDEAIADPTTHDEVRAEHDKVVAAGGYGVPTLFFPDGQC
                     FFGPVIIDPPTEGEGALRLWHAVTAWLEFPHLYELQRPKTEADGRFIAQTFKPYLEAR
                     DWVSIDRGKVVDLDRMADQPD"
     gene            complement(3795..4682)
                     /locus_tag="VLR27_07385"
     CDS             complement(3795..4682)
                     /locus_tag="VLR27_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HSP03304.1"
                     /translation="MPEAAEVAVAAAQLHQVAAGRELTALPVTHPRTVRHTDPAAVAA
                     LVGRHVDAVGSHGKWIVVDLDGSDAALGIHLRMSGQLLVAEPGTPHPDRHVHATLALG
                     PHPDPFWPTDVPSGGHGCRPERGVEVWFRDPRTFGELRVLPDGVAPVASDLFDPAVTG
                     SSLHSLAARRRVGVKAVLLDQTQGVAGIGSYLADEALHRAGIDPTVPSGQLAVPAWER
                     ILVAARAIATDSVAAGGVTLEDEGWVDLWGRPGAYAAELQVHARAACAACGTATSSAV
                     VGGRRARWCRRCQHPRRRR"
     gene            complement(4691..5026)
                     /locus_tag="VLR27_07390"
     CDS             complement(4691..5026)
                     /locus_tag="VLR27_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03305.1"
                     /translation="MSANGSNGQNGSSNGDAGGRKRRNRKRRNRSTAKATAEFWGDPK
                     TLPEPRDDIRITKDPSAVVRSLGRPPLPGQEQAAEAYFAAVYARAVGLASALAAVGEL
                     IEPEELLEG"
     gene            complement(5023..>5478)
                     /locus_tag="VLR27_07395"
     CDS             complement(5023..>5478)
                     /locus_tag="VLR27_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HSP03306.1"
                     /translation="LTDRYDLPIACYHGGLGRGARDELVKTFQNGTGPGAMVLSLKAG
                     GTGLNLTAANHVVLYDRWWNPAVEDQARDRVWRIGQKSTVIAHRLVCPGTVDERVEEV
                     VAGKRRIADLVLPKSSSIGDLDAQQLQAALGIDPDLLLTDDDDTAEDAA"
BASE COUNT          793 a         1992 c         1962 g          731 t
ORIGIN      
        1 aggtcgagca caaggaggcg gcgcgcgccg ggctcgtcga ggtcgggaag accgtggacc
       61 tcggtgcgac ccagctgtgg acccgggtca acgcgctcga gtcaccgtgg ctgctcgacg
      121 atctgaccac cctcgtcggc gagatcggtg acgtgctcga cgtcgtcatg gtgccgaagg
      181 tcgagggtcc ggaggacatc cactaagtgg accgtctact cgcccagttg gaggcgaagg
      241 cgggcctgac ccggccgatc ctggtccacg cgatcctgga gaccggacgc ggcatggcca
      301 acgtggagga gatctgtctc gcctcaccgc ggatgcaggg cctgtcgctc ggacccgccg
      361 acctcgcggc cgagcgacgg atgaagacca cccgcgtggg cggtggacat ccgggctacc
      421 tggtccgcca ggatcccggt gcggacggcg acatcgcggc agccgggcgc gcgaccgccc
      481 agcaggacct gtggcactac acggtggccc gcatggtcga cgcgtgcgtc accgcgggga
      541 tcgcgcccta ctacggcccg ttcggcgaca tcaaggacac ggtcgcctgc gaggaccagt
      601 tccgcaacgc ctacctgctc ggctgcgtgg gcacctggac gctgcacccg gtgcagatcg
      661 acatcgccaa gaaggtggtc tccccgccgg tcgacgacgt ggtgcacgcc aagcgcgtcg
      721 tggaggccat gggcgacggc accggtgccg tgatgctcga cgggaagatg gaggacgacg
      781 cctcggtgaa gcagtgcctc gtcgtgctcg acctggccaa ccagctcgcc gagcgagacc
      841 ccgagctcaa ggaggcctac ggcctgtgag cgcgaccgaa gcgacgctgc ggccccgccg
      901 ttccgtcctc tacatgcccg gcgccaacga gcgggcgctg gagaaggcca agggcctcac
      961 cgccgacgcg ctcatcctcg acctcgagga cgccgtcgcc cccgacgcca aggccgaggc
     1021 ccgcgaccgc gtgtgcgcgg ccgccgcctc cggcgagtac ggcatgcgcg agatcgccat
     1081 ccgggccaac ggcctcgaca cccagtggca cgcggacgac gtcgccgcca tcgcggaggc
     1141 cggccccgac gccatcgtca tccccaagat caactcggtc gaggacgtcc gggccatcga
     1201 gtcggccctc gactcggccg gcgcccccga ccgcacgaag atctgggcca tggtcgagac
     1261 gccgatcgcc atgctccacg ccgaggagat cgccggctgc tccgagcgcc tcagcgtgct
     1321 cgtcatgggc accaacgacc tggccaagga gctgcacgcc cagcacgtcc ccggccgcca
     1381 gcccctgctc accggcctcg gcctgtgcct gctcgccgcg cgggccaccg gcaaggtcat
     1441 cctcgacggc gtctacaacg acatcaagga cgaggcgggc ttcctcgccg agtgccgcca
     1501 gggcaaggag atgggcttcg acggcaagac gctcatccac ccgagccagc tcgacccgtg
     1561 caacgaggtc ttcgccccgt ccgaggacga ggtcgcctcc gcccgcgaga tcatcgccgc
     1621 cttcgaggag gccgagcgcg agggccgggg cgtcgtcacg gtcaacggcc ggatgatcga
     1681 gaacctccac gtcgaccagg cccgccagac cctggcccag gccgaggcca tcgccgccct
     1741 ggagtcctag gggttctggc agtcctcagg tcacaccggg aggtggatcc ggatcgtcgt
     1801 cccctccccc ggggccgtct ccacggacag gtcgccgtcc agctcggcga ccatcgccgt
     1861 gaccatgctc agcccgaggc ccagcccggc cacggtggtg gggttgcgga ccgagcgcga
     1921 gaacgcctcg ccccgtcggt cgagcacgtc gtcgggcagg cccgggccgt ggtcgcacac
     1981 ctcgagcacg ccggcatgcg cgaccacctc ggccccaccg gcgtagagca gcgcgttctc
     2041 caccagcgca cccagcgcga tccgcacgag gcggtcatcc gtcgtccact cgagtcccgg
     2101 atcgcagcgg acggtgacga gggaggggtc ggcgctggcg tccaccacct cttcgatcag
     2161 ctgccgcacc cggaccgggc ggtggacggc tcggacctcg ccggcgccga gctcgctcat
     2221 gacgatgagc tcctcgatga ggcgccccaa ccgtccgagg gcggcgtcgg cccggtcgag
     2281 cagcctgtcg gcgtccggca ggtccgggtg gaggttgcgg tgcatgtgca gcatcccggc
     2341 cgcgatggtc agcggcgtgc gcagctcgtg actcaccagg ttgatgaacc cgtgcttgag
     2401 cgcatccacc cggtcacgtt cgtgggactg gcgggcggac tcgatggcgg tggcggtctg
     2461 ggcggcaagg ccctcgagca acatgatgtc gacgtcgtcg aacgcgtccg ccacctcgct
     2521 gtcgacctgg agcagcccga tcggtcgtcc caggcagacg agcggcacgg ccaggaagct
     2581 gaccatgccg gcgttcgaac cggcccggcg gacctcggga tcgacccggt cgtcggcatc
     2641 caggtcgggg acgtagatcg tctcgccggt ggcgacgacc cggccgccga tgccggagcc
     2701 gaccggcaac cggacgtcga gcacctcggg tgacaccggc gggtccgatg cggcgatgcc
     2761 gaccgtcccg tgctcgacga ggctgacgct cccaccccgg aagtcgacca gctcgcgcat
     2821 ggcggcgagc acggccttga gggtggtctc gagctcgagc gagggcccca gccggtggct
     2881 cacgctggcg aggagtcgct gcgcctcggc gatgcgggcg aggcgctcct cggccgcacc
     2941 cggtgggccg gtcatgggcc gactgtaccg gcatgtgctg ctcggggtgc cagatccgcc
     3001 cgccggtacc ctccgaccat ggccgcgccg accaccccca ccgccgtcga cttccacttc
     3061 gacgtgatgt gcccgttcgc gtaccagacg tcgcggtgga tccgggcggt gcgggaccag
     3121 accgacctgg acatcacctg gcgcttcttc tccctggagg agatcaaccg ccgcgagggc
     3181 aagaagcacc cgtgggagcg ggactggtcc tacggctggt cgatgatgcg catcggcgcg
     3241 ctgctgcgcc ggcgggacat ggccgagctc gacgcctggt acgagcgagc cggtcgggcg
     3301 ctgcacgagg acggccacaa gccccacgat cccgacgtcg cccgccacct gctcaccgag
     3361 atcggcctcg acccgggcct ggtcgacgag gccatcgccg accccaccac gcacgacgag
     3421 gtccgagccg agcacgacaa ggtcgtcgcc gccggcggct acggcgtgcc gacgctgttc
     3481 ttccccgacg gccagtgctt cttcgggccg gtcatcatcg atccgcccac cgagggcgag
     3541 ggggccctgc gcctctggca cgcggtcacc gcctggctcg agttccccca cctgtacgag
     3601 ctgcagcgcc ccaagaccga ggccgacggc cgcttcatcg cccagacgtt caagccgtac
     3661 ctcgaggccc gggactgggt gagcatcgac cgcggcaagg tcgtcgacct cgaccgcatg
     3721 gccgaccagc ccgactgaaa atcccgttga tccgttccac cggtggtgac ggatccacgg
     3781 gatttccatg ggggtcagcg gcgccggcgg gggtgctggc agcgacggca ccagcgggcc
     3841 cgtcgaccgc cgacgaccgc cgagctggtc gccgtcccgc aggccgcgca ggccgcccgg
     3901 gcgtgcacct gcagctcggc ggcgtaggcg ccgggacggc cccacaggtc gacccacccc
     3961 tcgtcctcga gggtgacccc gccggcggcc accgagtcgg tggcgatggc ccgggccgcg
     4021 acgaggatcc gctcccacgc cgggacggcg agctggccgg acgggacggt cgggtcgatg
     4081 ccggcccggt ggagcgcctc gtcggccagg tacgagccga tgccggccac gccctgggtc
     4141 tggtcgagca gcaccgcctt caccccgacc cgacggcgtg cggccaggga gtgcagcgac
     4201 gagccggtca cggccgggtc gaacaggtcc gacgccaccg gcgcaacacc gtccggcagc
     4261 actcgcagct caccgaacgt gcgcgggtcc cggaaccaca cctccacccc acgttctggc
     4321 ctacatccat gaccaccaga cggcacatcg gtaggccaga acggatcggg gtgggggccc
     4381 agcgccaggg tggcgtggac gtgacggtcg ggatgcgggg tgcccggctc ggcgacgagg
     4441 agctggccgc tcatgcgcag gtggatgccg agcgcggcgt cgctgccgtc gaggtcgacc
     4501 acgatccact tgccgtggct gcccaccgcg tcgacgtgtc gtcccaccag cgcggcgacg
     4561 gcggccggat cggtgtggcg gacggtgcgc gggtgggtca cgggcagcgc ggtcagctcc
     4621 cggccggcgg cgacctggtg gagctgggct gcggcgaccg cgacctcggc ggcttcgggc
     4681 acgggcccgg tcagccctcg agcagctctt cgggctcgat cagctcgccg accgcggcca
     4741 gggcgctggc caggcccacg gctcgggcgt agacggcggc gaagtaggcc tccgcggcct
     4801 gctcctggcc gggcagcggc ggccgcccga gcgaccgcac gaccgcagac gggtccttgg
     4861 tgatgcggat gtcgtcgcgg ggttcgggga gcgtcttggg gtcgccccag aactcggccg
     4921 tcgccttggc ggtggaccgg ttgcggcgct tgcggttgcg gcgcttgcga ccgccggcgt
     4981 cgccgttgct cgagccgttc tggccgttgg atccgttggc gctcatgctg cgtcctccgc
     5041 cgtgtcgtcg tcatcggtga gcaacaggtc cgggtcgatg ccgagcgcgg cctgcagctg
     5101 ctgggcatcg aggtcgccga tgctcgagga cttgggcagg accaggtcgg cgatgcgccg
     5161 cttgccggcc accacctcct cgacccgctc gtccacggtg ccggggcaca cgaggcggtg
     5221 ggcgatgacc gtgctcttct ggccgatgcg ccacacgcgg tcccgggcct ggtcctcgac
     5281 ggccgggttc caccagcggt cgtagagcac gacgtggttg gcagccgtca ggttcagccc
     5341 ggtgccgccg gccttgaggg acagcaccat ggcgccgggc ccggtgccgt tctggaacgt
     5401 cttgaccagc tcgtcgcgag cgccgcggcc gagaccgccg tggtagcagg cgatgggcag
     5461 gtcgtagcgg tcggtgag
//
LOCUS       DATISU010000179         5169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_287991, whole genome shotgun sequence.
ACCESSION   DATISU010000179 DATISU010000000
VERSION     DATISU010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287991"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VLR27_07400"
     CDS             complement(<1..551)
                     /locus_tag="VLR27_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HSP03307.1"
                     /translation="MGESITITDNRTGESVEIPIEYGGISSEPFKKLLPGIWFEDEAF
                     TATAATRSAITDIDGEAGILRYRGYPIEQLAEHSTYLEVAYLLIFGELPTTEQLEQWT
                     HDITYHTYIHENMRKRFLEGFHYDAHPMGMLVSSVAALSTFYENAKDIFDEEVRYKQI
                     TRLIAKMPTLAAAAYRFSVGMPFV"
     gene            complement(618..1565)
                     /locus_tag="VLR27_07405"
     CDS             complement(618..1565)
                     /locus_tag="VLR27_07405"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSP03308.1"
                     /translation="MAMDLRQLTALLAVAEHGSFSAAARAIHTVQSNVSTHVARLEKE
                     LGVPLVDRSSGGLTEEGEAAVARARRIRSELDALQSDVAALRAEVVGHVRVGMIGSIA
                     RWLAPSLLAGMQERHPGVELVIVDATTTSLLPQLLEGSLDLAVVNLPVADPEIGAEPL
                     FAEERILVVPGGHDLAFDARDETSVADEVSFARLAEHRLVLEPKGTAFRDELDRVAAE
                     VGVELHPLAEVDGMMLVASLAFGGFGPAILPTTATLEEPPGDWVRVHVPELLHRDVGL
                     ARRRSGRLAAPARALRELLHELVAAEAPRHPGVDLPEPV"
     gene            1862..3292
                     /locus_tag="VLR27_07410"
     CDS             1862..3292
                     /locus_tag="VLR27_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HSP03309.1"
                     /translation="MSTPAAFFDLDRTLLRGASGPILTGALRAAGVISERVIPGENLL
                     YRLFNAVGENRPAMLLARQGARFSKGQKRTDVQHAGELAAELLVPMVGAYAAPIIEKH
                     REEGRPVVLATTSPYDLVKPFADQLGLDDVIATRYGTDSDGHYDGTINGHFVWGPGKL
                     SACEEWAEANEVDLDESWAYSDSFYDIPLLSAVAHPRVVNPDPRMFALATLRRWPIRY
                     LDKPEGVPKLLGMVEPAEALLPFARPELVPYARFDIAGTENIPAEGGCIVVANHRSYF
                     DTLAMALTISKTGRPVRFLGKKEVFDAPVVGQLATAMGGIRVERGSGSDEPLRAAAEA
                     VLAGELVALMPQGTIPRGPAFFDPELKGRWGAAKLAAMTKAPVIPVGMWGTEQVWPRS
                     SRLPNLLNVTNPPLVTVRVGKPVDLKYRSPDPDTKRIMTAISELLPKESRKKRNPTAE
                     ELARTYPPGYEGDPGSELERRPGTDT"
     gene            complement(3335..4345)
                     /locus_tag="VLR27_07415"
     CDS             complement(3335..4345)
                     /locus_tag="VLR27_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03310.1"
                     /translation="MNCTNGHENADDQAFCGTCGVPLTPPDPAVDARSSTASPPTRKH
                     PPLVIVAYAALAAAFVGSFLPWARVLFVTVNGTDGDGVITALTAAGAALCMFLGTREE
                     PAQRKLLVAAFLLSIACAGVYLYDANQLVREYGEDAGMFGEITVQPQMGLYLGALGSI
                     LAVGSTGIRAAQEHRPGILDGLPAPTYVALAAALYAIALIAVPGWWGLTTLAALIAIS
                     VGFVQRRQAAAGSTSLRPATFALAAGIVGLLGGIGGFVIDGDQGTDIAECTEVFADGA
                     TTQRSWDEDGVTCLDRDGERTFTFTMEAFCWEQDEAPLANDYGWGYVDEPWHAGEDAP
                     DC"
     gene            4532..>5169
                     /locus_tag="VLR27_07420"
     CDS             4532..>5169
                     /locus_tag="VLR27_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSP03311.1"
                     /translation="MVVEPALELAELLAVDVSSLPLRQWVEHQAAIERRRNQLDAALL
                     RRTAEFDQSGAWQLDAATSATNWFHGQVGGARSVMASRVKLAAHLELMPVTREAFEDG
                     DITESHAKVLSRCVANPRVRDRFAEDEVELVAKAMDLDADKLATEVDAWIRLHDQDGV
                     EPHDPEHDTVHANRVGDRVKINADLGLESGLPVLAALTERHDEIWRRDRSVV"
BASE COUNT          787 a         1807 c         1836 g          739 t
ORIGIN      
        1 acgaagggca tgccgacgct gaagcggtag gccgcggcgg ccagcgtggg catcttggcg
       61 atgaggcggg tgatctgctt gtagcggacc tcctcgtcga agatgtcctt ggcgttctcg
      121 tagaaggtgg agagcgcggc caccgaggag accagcatcc ccatcgggtg ggcgtcgtag
      181 tggaagccct cgaggaaccg cttgcgcatg ttctcgtgga tgtaggtgtg gtacgtgatg
      241 tcgtgcgtcc actgctcgag ctgctcggtg gtgggcagct cgccgaagat cagcaggtag
      301 gcgacctcga ggtaggtgga gtgctcggcc agctgctcga tcgggtagcc ccggtagcgc
      361 aggatgccgg cttcgccgtc gatgtcggtg atggcgctgc gggtggccgc ggtggcggtg
      421 aaggcctcgt cctcgaacca gatccccggc aggagcttct tgaacggctc cgagctgatg
      481 ccgccgtact cgatcgggat ctcgacggac tcgcccgtgc ggttgtcggt gatcgtgatg
      541 gactcgccca cgctgggtgc cccttgtgtc gcagtcggtc ggtggtcggg tcggagacta
      601 cgcggtggga gaggcggtca cacgggctcg ggcaggtcga cgcccgggtg gcgcggtgct
      661 tcggccgcaa ccagctcgtg gagcagctcg cgcagcgctc gggccggggc ggccaggcgg
      721 ccgctgcgcc ggcgggcgag gccgacgtcg cggtggagca gctcgggcac gtggacccgc
      781 acccagtcgc cgggcggctc ctcgagggtc gcggtcgtcg ggaggatggc gggcccgaag
      841 ccgccgaagg ccagggaggc gaccagcatc atgccgtcga cctcggcgag cgggtggagc
      901 tccacgccga cctcggccgc gacccggtcg agctcgtcac ggaacgcggt gcccttgggc
      961 tcgaggacca agcggtgctc ggccaggcgg gcgaaggaga cctcgtcggc gacggaggtc
     1021 tcgtcccggg cgtcgaaggc gaggtcgtgg ccgccgggca ccaccaggat ccgctcctcg
     1081 gcgaagaggg gctcggcccc gatctcgggg tcggccaccg gcaggttgac caccgccagg
     1141 tcgagggagc cctcgaggag ctgggggagg agcgacgtgg tggtggcgtc cacgatcacc
     1201 agctcgacgc cggggtggcg ctcctgcatg ccggcgagca ggctcggcgc cagccagcgg
     1261 gcgatggagc cgatcatgcc gacccggacg tggccgacca cctcggcccg cagggcggcg
     1321 acgtcgctct gcagggcgtc gagctcgctg cggatgcgcc gggcccgggc cacggccgcc
     1381 tcgccctcct cggtgagccc gccggaggag cggtcgacca gcggcacgcc cagctccttc
     1441 tcgaggcgag ccacgtgggt ggagacgttg gactgcaccg tgtggatcgc ccgggcggcc
     1501 gccgagaacg atccgtgctc ggcgacggcg agcagggcgg tcagctggcg cagatccatg
     1561 gccatctgtt tactggatgg taggtatctg ggagaactgt tggacagatg cttgcgcgtt
     1621 cggcacactg atcttcacaa cgccggagcg gatcgtcccc ccgaacgctc cgagcacagc
     1681 gtgcccgaca ccaaggatgt ccccccatcc gtcggatgca ccaaaattga ggtgtggagc
     1741 cggtccgtcc cccccttggg ccggctcccc tcagttttcc tccgaggttc gctccgaaca
     1801 gctgccagaa acgggcgaac gccggacacg acggcggccg cgccgggtac cgtccgaccc
     1861 gatgtcgacg cccgccgcct tcttcgacct cgaccgcacc ctgctccgag gcgcgagcgg
     1921 tccgatcctc accggcgcgc tgcgagcagc cggcgtcatc agcgaacggg tgatcccggg
     1981 cgagaacctg ctgtaccgcc tgttcaacgc cgtcggtgag aaccggcccg ccatgctgct
     2041 cgcccgccag ggtgcccggt tcagcaaggg ccagaagcga accgacgtcc agcacgccgg
     2101 cgagctggcc gccgagctgc tcgtgcccat ggtgggcgcc tacgccgcac cgatcatcga
     2161 gaagcaccgc gaggaaggtc ggccggtcgt cctcgccacc acctcgccct acgacctggt
     2221 gaagcccttc gccgaccagc tcggcctcga cgacgtcatc gccacccggt acggcaccga
     2281 cagcgacggc cactacgacg ggaccatcaa cggccacttc gtgtggggtc cgggcaagct
     2341 ctcggcctgc gaggagtggg ccgaggccaa tgaggtcgac ctcgacgagt cctgggccta
     2401 ctccgacagc ttctacgaca tcccgctgct gtcggcggtc gcgcacccca gggtggtgaa
     2461 ccccgacccg cgcatgttcg ccctcgccac gctgcgccgc tggccgatcc gctacctcga
     2521 caagcccgag ggcgtgccca agctgctcgg catggtcgag ccggccgagg cgctgctgcc
     2581 cttcgcccgc cccgagctgg tgccctacgc ccgcttcgac atcgccggca ccgagaacat
     2641 cccggccgag ggcggctgca tcgtggtcgc gaaccaccgc tcgtacttcg acacgcttgc
     2701 catggccctg acgatctcca agaccggccg acccgtgcgc ttcctcggca agaaggaggt
     2761 gttcgacgcg ccggtcgtgg gccagctggc caccgccatg ggtggcatcc gcgtcgagcg
     2821 gggctccggc tccgacgagc cactccgtgc cgccgccgag gccgtgctgg ccggcgagct
     2881 cgtcgccctc atgccccagg gcaccattcc ccggggtcca gcgttcttcg accccgagct
     2941 gaagggccga tggggcgccg ccaagctcgc ggccatgacc aaggcgccgg tgatcccggt
     3001 gggcatgtgg ggcaccgagc aggtctggcc ccgatcgtcg cgcctgccga acctgctcaa
     3061 cgtcaccaac ccgccgctgg tcaccgtgcg ggtggggaag ccggtcgatc tcaagtaccg
     3121 ctcgcccgat cccgacacca agcggatcat gaccgccatc agcgagctgc tgccgaagga
     3181 gtcccgcaag aagcggaacc ccacggccga ggagctggcc cgcacctacc cgccgggcta
     3241 cgagggcgac cccgggtccg agctcgagcg gcggccgggc accgacacct gagggcgcgg
     3301 tcgcctcacc gggagcgcac ctgtctctgg aggatcagca atccggcgcg tcctcaccgg
     3361 cgtgccacgg ttcgtcgacg tatccccacc cgtagtcgtt ggcgagcggc gcctcgtcct
     3421 gctcccagca gaacgcctcc atcgtgaagg tgaacgtgcg ctcgccgtct cggtccaggc
     3481 aggtgacgcc gtcctcgtcc cagcttcgct gggtcgtcgc gccgtcggcg aacacctcgg
     3541 tgcactcggc gatgtcggtg ccctgatcgc catcgatgac gaacccaccg atcccgccga
     3601 gcaacccgac gatgccggcg gccaaggcga acgtcgccgg acgaagcgac gtgctccctg
     3661 ccgccgcctg gcgtcgctgg acgaagccga ccgagatcgc gatgagggcc gcgagcgtgg
     3721 tgagacccca ccagccagga acggcgatga gcgcgatcgc atagagcgcg gcggcgaggg
     3781 cgacgtaggt cggcgcaggt agaccgtcga ggatgccggg gcggtgctcc tgggcagcgc
     3841 ggatccctgt gctgcccacc gcaaggatcg agcccaaggc accgaggtac agccccatct
     3901 ggggctgcac ggtgatctcc ccgaacatgc cggcgtcctc gccgtactcc cggacgagct
     3961 ggttcgcgtc gtacaggtac acaccggcac acgcgatgct gagcaggaac gcggcgacca
     4021 gcagcttccg ctgcgctggc tcctcccgcg ttcccaggaa catgcagagg gctgcgccgg
     4081 ccgcagtgag ggcggtgatg acgccgtcgc cgtccgtgcc gttcacggtg acgaacagca
     4141 cccgggccca ggggaggaac gagccgacga acgctgcggc gagcgcggcg taggcgacga
     4201 tgacgagcgg cgggtgcttt cgagtcggcg gcgaggcggt ggaggaccgg gcatccacgg
     4261 ctgggtccgg cggagtcagc ggcacgccgc aggtgccgca gaaggcctgg tcgtccgcgt
     4321 tctcgtgtcc gttcgtgcag ttcatgtgcc ccaccccgct ccgccgtact tctcgcacga
     4381 cggtacccag caatggcgcg gccgtcatgc atgcgcgccc cgacggccag ccctcggcat
     4441 cgccggacgg tgctgccgcc cccgatcggc cattgaggaa aatgtccgac ttgaggttgt
     4501 ctcgaactgg tgttcgctct atcctgaggt catggtcgtg gaaccggcgc tcgaactggc
     4561 ggagctgctc gccgtcgacg tgtcgtcgct gccgctgcgt cagtgggtcg agcaccaggc
     4621 cgccatcgaa cgccgccgca accagctcga cgccgctctg ctccggcgca cggccgagtt
     4681 cgaccagagc ggtgcctggc agctggatgc ggcgaccagc gccaccaact ggttccacgg
     4741 tcaggtcggc ggcgcccgga gcgtgatggc gagccgggtc aagctggctg cgcacctcga
     4801 gctcatgccc gtcacccggg aggcgttcga ggatggcgac atcaccgagt cccacgccaa
     4861 ggtgctcagc cggtgcgtgg ccaacccccg ggtgcgcgac cgcttcgccg aggacgaggt
     4921 ggagctggtg gccaaggcca tggacctcga cgcagacaag ctcgccaccg aggtcgatgc
     4981 gtggatccgc ctgcacgatc aggacggcgt cgagccgcac gaccccgagc acgacacggt
     5041 ccacgccaac cgggtgggcg accgggtgaa gatcaacgcc gacctcggcc tggagagcgg
     5101 gctgccggtg ctggcggcgc tcaccgagcg ccacgacgag atctggcggc gcgatcgttc
     5161 cgtggtcga
//
LOCUS       DATISU010000180         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_8571, whole genome shotgun sequence.
ACCESSION   DATISU010000180 DATISU010000000
VERSION     DATISU010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8571"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VLR27_07425"
     CDS             <1..496
                     /locus_tag="VLR27_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03312.1"
                     /translation="LGCGRVDAPGVPVGTLDGLGDAIASIRVRGWAIDPQTADPVRVE
                     IRRNGVLVATVEADQHRSDLGDRASRYGTAHGFDAAITPETGRNEVCVRVLGVGGGGD
                     ADAGCAQIDHAGPAAHASDAAPAPEVVEPVIAVVDPVLPEVVEAVAAPLQLVGDLGEG
                     LLGG"
     gene            complement(504..2318)
                     /locus_tag="VLR27_07430"
     CDS             complement(504..2318)
                     /locus_tag="VLR27_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP03313.1"
                     /translation="MDIGGFAAEALELLPVPFLALEAVSGASSQRDLRVVWANPSARE
                     RFGPGVEGAWLDRDQPLGVMESIVAEVERSRRTPRLSVIQRSVEREAAVSHVRCTVLP
                     IETGSAVIVEDVTAEVEARAELAGVGSALERVERWGNLGVWEVDLQAGTVYWSTQVYE
                     ILGVEDQSLDRFHEVVHPDDRALVDHVTRRLLDQPGPYRVTHRLVRNGEVRTVDQHMQ
                     SVPDATGRPVRLLGTMIDVTATRALQQQVQHGQQMRTIGLLAGGMAHDLANALLVMRG
                     HAEVLLTRADLDDEVRASLAAIVRGGEKASTLTRRFMALGRRDELRPTRVDLGDVVEE
                     VLDLAGPAMRSEVALSIEPTEPSPLVLADEHRLRQVLLDLIFNARDAGAQAITLRVSG
                     VELGPGDPRSTDTGLVPGRYGLIDVVDDGTGIDPATMERIFDPFFTTKDADSGSGLGL
                     ANAQDFAGQSLGAILVSSVLGSGTTMSLLLPATVAADVPSIPSGRRRARRVLVGASPD
                     RCAELRETLADVDAQLVCMEDLDGLAFSLRTEPIDVAVLDDSMLPASTWPGGLAGVPT
                     IVVTDRGSDYPQATLGVQPGDHAGLRTALGDLLPPSGR"
     gene            2651..>2858
                     /locus_tag="VLR27_07435"
     CDS             2651..>2858
                     /locus_tag="VLR27_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03314.1"
                     /translation="MSHQDGRRLRTDRSYPLGEGPQQAWDALARVDAYRRWWPWLRRF
                     DAERLASGERWEARIRIPMPWSLRF"
BASE COUNT          403 a         1047 c         1012 g          396 t
ORIGIN      
        1 cctcggctgc ggacgggtcg acgcccccgg cgtcccggtc ggcacgctgg acggcttggg
       61 tgacgcgatc gccagcatcc gggtgcgcgg gtgggcgatc gatccgcaga cggccgatcc
      121 ggtgcgggtg gagatccggc gcaacggtgt cctcgtcgcc accgtcgaag ccgaccagca
      181 ccgcagcgat ctgggtgacc gggcatcccg ctacggcacc gcccacggct tcgacgctgc
      241 catcacgccc gagaccggcc ggaacgaggt gtgcgtgcgg gtcctgggtg tgggcggagg
      301 cggcgacgct gacgccggct gcgcgcagat cgaccacgcc gggccggcgg ctcacgcctc
      361 ggacgccgcc ccagccccgg aggtggtgga gccggtcatc gccgtggtcg acccggtgct
      421 gcccgaggtg gtcgaggccg tcgccgcacc gctccagctg gtcggggacc tcggcgaggg
      481 cctcctcggc gggtgaccgg cggtcaccgc cccgacgggg gcaacagatc ccccagtgcg
      541 gtccgcaggc cagcgtggtc gccaggctgc acccccagcg tggcctgcgg gtagtcgctg
      601 ccccgatcgg tcacgacgat ggtcggcacg cctgccagcc caccgggcca ggtggacgca
      661 ggcagcatcg agtcgtcgag cacggcgacg tcgatcggct cggtccggag gctgaaggcg
      721 aggccgtcga ggtcctccat gcagaccagt tgggcgtcga cgtcggcgag ggtctcgcgc
      781 agctcggcgc accggtcggg ggatgcgccg accagcaccc ggcgggcgcg tcgccgaccg
      841 ctcgggatgg agggcacatc tgctgccacg gtggccggga gcaggagcga catcgtcgtt
      901 ccggagccca acacggacga gaccaggatc gcgccgagcg actggccggc gaagtcctgt
      961 gcgttcgcga gcccgagccc cgaccctgag tctgcatcct tcgtggtgaa gaagggatcg
     1021 aagatccgct ccatcgtggc cgggtcgatg ccggtgccat cgtccaccac atcgatcagg
     1081 ccgtagcgcc cgggcacgag ccccgtgtcg gtgctgcgcg gatcgcccgg acccagctcg
     1141 acgccgctga cccggagcgt gatcgcctgc gctccggcgt cccgagcgtt gaagatgagg
     1201 tcgaggagca cctgcctgag ccggtgctcg tcggcgagca cgagcggcga cggctcggtc
     1261 ggctcgatgg acagcgcgac ctcggatcgc atcgccggac cggccagatc gaggacctct
     1321 tccacgacgt ctcccaggtc gacgcgcgtg gggcggagct cgtcgcgtcg cccgagggcc
     1381 atgaagcggc gggtcaacgt cgaggccttc tcgccgccgc ggacgatggc cgcgaggctc
     1441 gctcgcacct cgtcgtccag gtccgcgcga gtcagcagca cctcggcgtg gccgcgcatc
     1501 accagtaggg cgttcgcgag gtcgtgagcc atgcccccgg cgaggagccc gatggtccgc
     1561 atctgctgcc cgtgttgcac ctgctgctgg agcgctcgcg tggccgtcac gtcgatcatc
     1621 gtcccgagga ggcgcaccgg tcggcccgtg gcatcgggaa ccgactgcat gtgctggtcg
     1681 accgtgcgga cctcgccgtt gcggacgagg cggtgcgtca cgcggtacgg gccgggctga
     1741 tccagcagcc ggcgggtcac gtggtccacc agcgcgcggt cgtcggggtg cacgacctcg
     1801 tgaaaccggt cgaggctctg atcctcgacg ccgaggatct cgtacacctg ggtcgaccag
     1861 tagacggtgc ctgcttgcag gtccacctcc cagacgccga ggttccccca tcgctcgacc
     1921 cgctcgagcg cgcttcccac tccggccagc tcggcgcggg cctcgacctc ggcggtgacg
     1981 tcctccacga tcacggccga acccgtctcg atcggcagga cggtgcagcg cacgtgcgac
     2041 accgccgcct cgcgctcgac cgaccgctgg atcacggaga gtcggggcgt gcggcggctg
     2101 cgctcgacct ccgcgacgat cgactccatc accccgagcg gctgatcccg atccaaccac
     2161 gccccttcga cgcccggacc gaaccgctcg cgtgcactcg ggttcgccca caccacccgc
     2221 agatctcgct gcgacgatgc tccgctcact gcctccagcg cgagaaacgg caccgggagc
     2281 agctccagcg cctcggcagc gaaacctccg atgtccattc gccctccaac acacgagctg
     2341 ccgccaggtc acctacgcac cggcccggcg aactggcgca ccgtaccgcc tcgatgacga
     2401 tttgggcggt atgcccgact cgaagtagct gagggcgacc acctcggcag tcctgcggtc
     2461 gagcgtcgtg aggtcgacgg accgcacctc ggcgtgccgg ccggcccgct cgtcgaaccg
     2521 gcgtgcccac acgtctggct gcgtctccgg cgagatcggc acgctcgacg gtcgcacgga
     2581 gcggttctac accacgtcgg atagacaacg tctctgtgcg ccgccccgac caccccctct
     2641 ggcgacgctg gtgagccacc aggacggccg gcggctgcgg accgatcgca gctacccgct
     2701 cggcgagggc ccgcagcagg cctgggacgc gctcgcccga gtggacgcct accgccggtg
     2761 gtggccgtgg ttgcgccggt tcgacgccga acggctggcg tcgggcgagc ggtgggaggc
     2821 ccgtatccgc atcccgatgc cgtggtcgct gcgcttca
//
LOCUS       DATISU010000181         3186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_288021, whole genome shotgun sequence.
ACCESSION   DATISU010000181 DATISU010000000
VERSION     DATISU010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288021"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VLR27_07440"
     CDS             complement(<1..721)
                     /locus_tag="VLR27_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HSP03315.1"
                     /translation="MAELPEVETLRRQLERDVVGRKVKTVDVHDTKVIPRHSTKKQFA
                     GMLEGAKLKSLIRRGTFLIFTLDTGDRLIVRPGLGGQLRKTASKDDLADGTAVVISFT
                     QGGQLRYIDPRRTGELWLLTPEQVAEGVDEIELLGYDVVDQPVPWADFAKLVLQRSVK
                     LKTLLTDEDLLTGIGNVYSDEILYDAGLRYDRLSDSLSVQEVRRLYRSVVEILHDAIK
                     HGGTTLTDDGWHDLHGDVGGYT"
     gene            complement(714..1487)
                     /gene="rnc"
                     /locus_tag="VLR27_07445"
     CDS             complement(714..1487)
                     /gene="rnc"
                     /locus_tag="VLR27_07445"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HSP03316.1"
                     /translation="MATAPGGTHRPTLLGRDRLLDALGYAPTDEALLAQAFAHRSWCA
                     EHAEYASNERLEFLGDSVLGFLVADHVYRTDAALDEGALTDVRKAVVNSVCLAEVAIE
                     LGLGEHLRLGKGEEQSGGREKPSILADAMEAVLGAVYLDGGLAAARRLVLDLLGERIQ
                     AAIDAGGGDQDHKSRLQERLATVTTATATYAFDATGPDHARHFTVTVTAGGHELGVGT
                     GRSKKQAEQQAARAALELLGDDGAGYPGAPDALGGLTDG"
     gene            complement(1508..1795)
                     /locus_tag="VLR27_07450"
     CDS             complement(1508..1795)
                     /locus_tag="VLR27_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HSP03317.1"
                     /translation="MPAETHVEGDKMDRNEVLTLIRDRLADILEIEPDSIKEGDSFVD
                     DLDADSLALIELVEGLEEEIGERTVGFRIEDEDLEDLKTVRDAVDYVVARL"
     gene            complement(1909..2892)
                     /gene="plsX"
                     /locus_tag="VLR27_07455"
     CDS             complement(1909..2892)
                     /gene="plsX"
                     /locus_tag="VLR27_07455"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HSP03318.1"
                     /translation="MLPIAIDANGGDRSPADIVEGARQARDELGVEILLVGTSEVADL
                     AGDLPLLEASEVIAMDADPGTSVRRMKDSTLVRAAEAVRDGKASAMISAGNTGATMAS
                     ALLRMGRLKGVKRPAIATPLPVPSGGHTVLLDAGANAECQPDWLVQFAQMGAAYARAR
                     YDVAEPRVGLLSIGEEPGKGDTLRKETYELLVADGVLDAAGGRFIGNVEGRDILTDAV
                     DVVVTDGFTGNVALKTLEGGMKGLVNRLLEAFSANDDTRAAADVLMPELLPLYATLDP
                     ENTGGAMLLGVDGVCIISHGSSGPTAVVNAVRVARDMVAGEVPAHLAAAVA"
     gene            complement(2915..3094)
                     /gene="rpmF"
                     /locus_tag="VLR27_07460"
     CDS             complement(2915..3094)
                     /gene="rpmF"
                     /locus_tag="VLR27_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HSP03319.1"
                     /translation="MAVPKKKTSKAKGRSRQAANWRLSRPSRSLCPRCGVVKVPHVVC
                     PNCGWYHGRQAVEVD"
BASE COUNT          423 a         1066 c         1207 g          490 t
ORIGIN      
        1 cggtgtagcc gccgacgtcg ccgtgcaggt cgtgccagcc gtcgtcggtg agcgtggtgc
       61 cgccgtgctt gatggcgtcg tggaggatct cgaccacgct gcggtagagg cggcggacct
      121 cctgcacgga cagcgagtcg ctcaaccggt cgtagcgcag gccggcgtcg tagaggatct
      181 cgtcggagta gacgttgccg atgccggtga gcaggtcctc gtcggtgagc agggtcttca
      241 gcttcaccga ccgctggagc acgagcttgg cgaagtcagc ccacgggacg ggctggtcga
      301 ccacgtcgta gccgagcagc tcgatctcgt cgacgccctc ggccacctgc tcgggcgtga
      361 ggagccacag ctcgccggtg cggcgggggt cgatgtagcg gagctggccg ccctgggtga
      421 agctgatcac caccgcggtg ccgtcggcga ggtcgtcctt cgaggcggtc ttgcggagct
      481 ggccgccgag gccgggccgc acgatgaggc ggtcgccggt gtcgagggtg aagatcagga
      541 acgtcccgcg ccggatcagg gacttgagct tggcgccctc gagcatcccg gcgaactgct
      601 tcttcgtgct gtggcgcggg atgaccttgg tgtcgtgcac gtcgacggtc ttcaccttgc
      661 gccccaccac gtcgcgctcg agctggcggc gcagcgtctc gacctcaggc agttcagcca
      721 tctgtgagcc ctcccagggc atcgggggcg ccggggtagc cggcgccgtc atcgccgagc
      781 agctcgaggg cggcacgggc cgcctgctgc tcggcttgct tcttggatcg gccggtgccg
      841 acgcccagct cgtgcccgcc ggcggtcacc gtgacggtga agtgccgggc gtggtccggg
      901 ccggtggcgt cgaaggcata cgtggccgtg gcggtggtga cggtggcgag gcgctcctgc
      961 aagcggctct tgtggtcctg gtcgccgccg cccgcgtcga tggcggcctg gatgcgctcg
     1021 ccgagcaggt cgaggaccag gcgccgggca gcggcgaggc cgccgtcgag gtagacggcg
     1081 ccgaggacgg cctccatggc gtcggccagg atcgacggct tctcgcgccc cccggactgc
     1141 tcctcgccct tgccgaggcg gaggtgctcg ccgagaccga gctcgatggc gacctcggcc
     1201 aggcacacgg agttcacgac ggccttgcgc acgtcggtga gggcaccctc gtcgagggcg
     1261 gcgtcggtgc ggtacacgtg gtcagcgacg aggaagccga ggacggagtc gcccaggaac
     1321 tcgaggcgct cgttgctcgc gtactcggcg tgctccgcgc accaggaccg gtgggcgaag
     1381 gcctgggcca gcagcgcttc gtcggtgggc gcgtagccga gcgcgtcgag gaggcggtcg
     1441 cgtccgagca gcgtcgggcg gtgcgtgccg ccgggcgcag tcgccacgga tccggaccgg
     1501 gttccgatca gaggcgcgcg acgacgtagt cgacggcgtc ccggacggtc ttcaggtcct
     1561 cgaggtcctc gtcctcgatc cggaagccga ccgtgcgctc gccgatctcc tcctcgaggc
     1621 cctcgaccag ctcgatcagc gcgagcgagt cggcgtcgag gtcgtcgacg aagctgtcgc
     1681 cctccttgat ggagtcgggc tcgatctcga ggatgtcagc gaggcgatct cggatgaggg
     1741 tcaggacctc gttgcgatcc atcttgtcgc cctccacgtg ggtctctgcg ggcaacgggt
     1801 gcccctcctg ggtcggaagc ttctctggcg accggcaacc atagccggac atgccggaaa
     1861 agccccggat gacgcctgaa tgcccaggtc agcgggtatg cgcgccggtc aggcgaccgc
     1921 ggccgcgagg tgcgccggga cctcgccggc caccatgtcg cgggcgaccc gcacggcgtt
     1981 gaccaccgcc gtcgggcccg aggacccgtg gctgatgatg cagaccccgt cgacgccgag
     2041 cagcatggcc ccgccggtgt tctccgggtc gagggtggcg tacagcggca gcagctcggg
     2101 catgaggacg tcggcggcgg cgcgggtgtc gtcgttggcg ctgaaggcct cgaggagccg
     2161 gttcaccagg cccttcatgc cgccttcgag cgtcttgagg gccacgttgc cggtgaagcc
     2221 gtcggtgacg accacatcga cggcgtcggt gaggatgtcc cggccctcga cgttgccgat
     2281 gaagcggccg ccggcggcgt cgagcacccc gtcggccacg agcagctcgt aggtctcctt
     2341 gcgcagcgtg tcgcccttgc ccggctcctc gccgatggac agcaggccca cccgcggctc
     2401 ggccacgtcg tagcgggccc gggcgtaggc ggcccccatc tgggcgaact gcacgagcca
     2461 gtcgggctgg cactcggcgt tggccccggc gtcgagcagc acggtgtgcc cgccggaggg
     2521 caccggcagg ggcgtggcga tggcggggcg cttgacgccc ttgagccggc ccatgcgcag
     2581 cagcgccgag gccatggtgg cgccggtgtt gccggccgag atcatggccg aggccttgcc
     2641 gtcgcgcacc gcctcggcgg cccgcaccag ggtggagtcc ttcatccggc gcacgctggt
     2701 gccggggtcg gcgtccatgg cgatgacctc ggacgcctcg agcagcggga ggtcgccggc
     2761 caggtcggcg acctcggagg tgccgaccag caggatctcg acgccgagct cgtcgcgcgc
     2821 ctggcgcgcg ccctcgacga tgtcagccgg tgaccgatcg ccgccgttgg cgtcgatcgc
     2881 tatgggaagc atgggcactg aaacccgaag ggtttcagtc gacttcgacg gcctggcgac
     2941 cgtggtacca cccgcagttg gggcagacga cgtgggggac cttcaccacg ccgcagcgcg
     3001 ggcagaggct gcgcgacggg cggctgagac gccagttcgc ggcctgacgg ctgcggccct
     3061 tcgccttgga ggtcttcttc ttggggacgg ccatggctgt tcaacctcag atgcggtgcc
     3121 gggagggcgt tcgaatgcgt ccgggccagt cggttgccga ccaggcggac gcgaaggaga
     3181 caccct
//
LOCUS       DATISU010000182         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_148562, whole genome shotgun sequence.
ACCESSION   DATISU010000182 DATISU010000000
VERSION     DATISU010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148562"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VLR27_07465"
     CDS             <1..193
                     /locus_tag="VLR27_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03320.1"
                     /translation="RPLLDAFTFDHGSDGVTRLVGEIGDRSHLHGVLAHLTSVNVDVV
                     SIGPAPTTPDAPTTHGAQT"
     gene            190..909
                     /locus_tag="VLR27_07470"
     CDS             190..909
                     /locus_tag="VLR27_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSP03321.1"
                     /translation="MITVTGLTKHYGDRIAVDDLGFDLAAGRVTGFVGPNGAGKSTTM
                     RMMVGLTRPDRGEVRYDGVRYGDLEHPARMVGSVLDARCMHPGRTARNHLRASAALSG
                     IPARRVDEVLADVGLESVAGDKAGGFSLGMRQRLALATALLGDPRVLLLDEPSNGLDP
                     DGIRWMRNHLRAFADAGGTVFVSSHLIAELARFADDLVVVGAGRLIAAETVDSLTART
                     GLSLEDSLLEMTSDSAEFASA"
     gene            906..1766
                     /locus_tag="VLR27_07475"
     CDS             906..1766
                     /locus_tag="VLR27_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSP03322.1"
                     /translation="MTTASRTTTATQEEVPMTTTELPLPTDRIVAAPARRRDLAQLPA
                     VLRSEWIKATTVRTNKTILVAAAVVGLLTSWATAVFLKDHDLIAVEVATGATALTAVL
                     ASIAGILLFSAEAQHGTLASALTAQPARWPVVAGKAVVAIGLGLALGVIGLLSGFAGS
                     LAGGLEVGYTSGVPSMVLWALLYTCGSAVLGLGVGMVVRHSAGAVSGLLVWWLVVEGI
                     ILQFAPGEVIWFVPFDTGWRSLGIESEFDPPEVVANGLANAAHASIFWGYVLAALVIG
                     AVLVHRRDVD"
     gene            complement(1782..>2599)
                     /locus_tag="VLR27_07480"
     CDS             complement(1782..>2599)
                     /locus_tag="VLR27_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HSP03323.1"
                     /translation="AATEMGLTYIHSTAASHDFEQVAEASGDGSRWYQLYYPTDPDVT
                     ESFLDRAKATGYETLVVTLDTTLLGWRPADLDRGFLPFLSNVGVANYLSDPAFRSKLE
                     RTPEEDPGSATFLWAGMFPNPGLSLDDLPFLRERWDGPIVLKGIIHPDDARAAVDAGV
                     EGIVVSNHGGRQVDGAMGALDALPRVVEAVGDRATILFDSGIRTGTDVIKALALGAEA
                     VLLGRPFLYGLALDGQAGVEHVLRCLLAELDLALTLGGYPSIADLGPEALERF"
BASE COUNT          341 a          965 c          916 g          377 t
ORIGIN      
        1 gcggccgctg ctcgacgcct tcaccttcga ccacgggagt gacggcgtca cccgcctcgt
       61 cggggagatc ggcgaccggt cgcacctgca cggcgtcctc gcccacctca cgtcggtgaa
      121 cgtcgacgtg gtctcgatcg gaccggcccc caccacccct gacgccccca ccacccacgg
      181 agcacagaca tgatcaccgt caccggactc accaagcact acggcgaccg gatcgccgtc
      241 gacgacctcg gcttcgacct ggccgctggt cgggtcaccg gcttcgtcgg cccgaacggc
      301 gccggcaagt ccaccaccat gcggatgatg gtcgggctca cccggcccga ccgtggcgag
      361 gtccgctacg acggcgtccg ctacggcgac ctggagcacc cggcccgcat ggtcggatcc
      421 gtcctcgacg cccgctgcat gcacccgggc cgcaccgccc ggaaccacct gcgcgccagc
      481 gccgcgctca gcggcatccc ggcccgccgg gtggacgagg tgctggccga cgtcggcctc
      541 gagagcgtgg ccggcgacaa ggccgggggc ttctccctcg gcatgcgcca gcggttggcc
      601 ctcgccaccg cgctgttggg ggacccccgg gtcctcctgc tcgacgagcc gtccaacggc
      661 ctcgacccgg atggcatccg ctggatgcgg aaccacctgc gcgccttcgc cgacgccggc
      721 gggacggtct tcgtgtcgag ccacctcatc gccgagctcg cccggttcgc cgacgacctc
      781 gtcgtggtcg gcgccggtcg gctcatcgcc gccgagaccg tcgactccct caccgctcgg
      841 accggcctgt cgctcgagga cagcctcctc gagatgacgt ccgactccgc cgagttcgcc
      901 tcggcatgac caccgcatcc cgcaccacca ccgccaccca ggaagaggtt cccatgacca
      961 ccaccgagct cccgctcccc accgaccgca tcgtcgcggc ccctgctcgc aggcgagacc
     1021 tcgcccagct cccggcggtc ctgcgcagcg agtggatcaa ggccaccacc gtgcggacca
     1081 acaagacgat cctcgtcgcg gccgcggtcg tcgggctcct gacctcctgg gccacggcgg
     1141 tgttcttgaa ggaccacgac ctgatcgcgg tcgaggtcgc caccggcgcg accgcgctca
     1201 ccgccgtgct cgcgtccatc gccggcatcc tgctgttcag cgccgaggcc cagcacggga
     1261 ccctcgccag cgccctgacg gcacagccgg cgcgatggcc ggtcgtggcg ggcaaggccg
     1321 tcgtcgccat cggcctcggt ctggcgctgg gcgtgatcgg tctcctcagc gggttcgccg
     1381 gttccctcgc cggcgggctc gaggtcgggt acacgagcgg ggtgccgtcg atggtgctct
     1441 gggcgctgct ctacacgtgc ggctcggccg tgctcgggct tggcgtgggg atggtggtgc
     1501 gccacagcgc gggcgccgtc tcgggcttgc tcgtctggtg gctggtcgtc gagggcatca
     1561 tcctccagtt cgccccgggc gaggtgatct ggttcgtgcc gttcgacacc ggctggcggt
     1621 cgctcgggat cgagtcggag ttcgacccgc cggaggtggt cgcgaacggc ctcgcgaacg
     1681 ccgcgcacgc atcgatcttc tggggctacg tcctcgctgc gctcgtgatc ggtgccgtcc
     1741 tcgtgcaccg gcgcgacgtc gactgatggg tggcccgcgg ctcagaagcg ctccagcgcc
     1801 tccgggccga ggtcggcgat gctcgggtag ccgccgaggg tgagagccag gtcgagctcg
     1861 gcgagcaggc agcgcagcac gtgctcgacg ccggcctggc cgtccagcgc cagcccgtag
     1921 aggaagggcc ggccgagcag cacggcctcg gcgccgaggg ccagggcctt gatgacgtcg
     1981 gtgccggtgc ggatgccgga gtcgaacagg atcgtggccc ggtcgccgac cgcctccacg
     2041 acccggggca gcgcgtcgag cgcacccatg gcgccgtcga cctggcggcc gccgtggttg
     2101 gacacgacga tgccctccac gccggcgtcc accgccgccc gggcgtcgtc ggggtggatg
     2161 atgcccttca gcacgatggg accgtcccag cgctcccgca ggaagggcag gtcgtcgagg
     2221 ctgaggccgg ggttggggaa catcccggcc cacaggaacg tggccgagcc cgggtcctcc
     2281 tccggcgtgc gttccagctt cgagcggaag gccgggtcgg acaggtagtt ggccacgccg
     2341 acgttggaga ggaacggcag gaagccccgg tcgaggtcgg cggggcgcca gccgagcagc
     2401 gtggtgtcga gggtgacgac cagggtctcg tagccggtgg ccttcgctcg atcgaggaag
     2461 ctctcggtca cgtccggatc ggtcgggtag tacagctggt accagcggga gccgtcgccg
     2521 ctcgcctcgg ccacctgctc gaagtcgtgc gacgcggccg tggagtggat gtaggtgagg
     2581 cccatctcgg tggcggccc
//
LOCUS       DATISU010000183         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_521212, whole genome shotgun sequence.
ACCESSION   DATISU010000183 DATISU010000000
VERSION     DATISU010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521212"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..930)
                     /gene="rsmA"
                     /locus_tag="VLR27_07485"
     CDS             complement(112..930)
                     /gene="rsmA"
                     /locus_tag="VLR27_07485"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HSP03324.1"
                     /translation="MTLGAGRIRALLDDHGITPSRALGQNFLIDPNTARRIAAASGAG
                     EGDAVLEIGPGLGSLTLALAELGADVTAVELDRHLVPVLREVVADAEPPVRVVEADAL
                     EVDLAELVTPGRRWRLAANLPYNVGTTIFLRVLQEAPEITGGVVLVQREVADRLAAGA
                     GDDAYGIPSVKAAWWVHVRVAGRVPAAVFHPRPRVDSAFVTFERHEPPGDEQLRQRVF
                     SLVDAGFGQRRKMLRRSLVDHIEPGALERADIDPTRRAEELALDDWVRLAAPPS"
     gene            complement(927..1208)
                     /locus_tag="VLR27_07490"
     CDS             complement(927..1208)
                     /locus_tag="VLR27_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03325.1"
                     /translation="MEDENPAPPDRRRLWVAVAATMVAFPLLLLDNLTADADDEPDGS
                     TSATKRAIVTVVVPLDRPETGPAKGISVVTSSTTSSTVPITAATTTSAP"
     gene            complement(1299..2057)
                     /locus_tag="VLR27_07495"
     CDS             complement(1299..2057)
                     /locus_tag="VLR27_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HSP03326.1"
                     /translation="MSGELRWTDDHCHLPDDDSVGEVLAAARAAGVERLVHVGTDLGS
                     SRSAMALAAAHDGVWATAGVHPHEARHGVDGIEELLTDPSCVAVGECGFDFHYDHSPR
                     DVQRDVFAAQVALAHAHDKALVIHTREAWEETFEVIDAEGVPERTVFHCFTGGADEAD
                     ECLARGAFLSFSGIVTFPSADDVRAAAARCPLDRMLVETDSPYLAPVPKRGRTNEPGW
                     VPLVGAGLAEASGHSVAAVAEATWRNAEHCYRLA"
     gene            complement(2062..>2521)
                     /locus_tag="VLR27_07500"
     CDS             complement(2062..>2521)
                     /locus_tag="VLR27_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HSP03327.1"
                     /translation="LGNLLSRVATVVAKKCDGIGPAPDPDSPLAAVAAETYADTAAAW
                     DRIQPSVALDATWRLIKETNAHLETNEPWKMDPGPEVERIMGDALEALRIVTVLASPA
                     VPASCREIWRRIGLPGTPEDQRLPDAATWGQYPGGLAVEKGDPLFPRIKA"
BASE COUNT          341 a          887 c          970 g          323 t
ORIGIN      
        1 gagctcggtg gcccggaagc ggtcgtggtc gagctgctcg aacagcgagg tgtcagccac
       61 ggcggccgat cctgccaggt ccgaccgcgc gcgacaccac gactgccagt ttcaggaggg
      121 gggagccgcc aggcggaccc agtcgtcgag ggcgagctcc tcggcgcggc gggtggggtc
      181 gatgtccgcc cgctccaggg ccccgggctc gatgtgatcg accaggctgc ggcgcagcat
      241 cttgcggcgc tggccgaagc cggcatcgac gagcgagaag acccgctggc ggagctgctc
      301 gtctcccggc ggctcgtggc gctcgaaggt gacgaaggcg gagtcgacgc ggggtcgcgg
      361 gtggaacacc gcggcaggga cgcggccagc gacgcggacg tggacccacc acgcggcctt
      421 caccgagggg atgccgtagg cgtcgtcgcc ggccccggcc gcgaggcggt cggcgacctc
      481 tcgctggacc agcacgactc cgccggtgat ctccggcgcc tcctgcagca cccgcaggaa
      541 gatcgtggtg ccgacgttgt agggcaggtt cgcggccagc cgccatcgcc gacccggggt
      601 gaccagctcg gcgaggtcga cctcgagggc gtcggcctcc acgacccgga ccggcggctc
      661 ggcgtcggcg accacctcgc ggaggaccgg caccaggtgc cggtcgagct cgaccgcggt
      721 gacgtcggcg ccgagctcgg cgagggcgag cgtcaacgac ccgagcccgg gtccgatctc
      781 caggacggcg tcgccctcac ccgcaccgct ggccgcggcg atgcgtcggg cggtgttcgg
      841 atcgatgagg aagttctggc cgagggcgcg gctcggcgtg atgccgtggt cgtcgaggag
      901 cgcccggatg cgcccggcac cgagggtcac ggagccgagg tcgtcgtcgc agcggtgatg
      961 gggacggtgg acgacgtggt ggacgacgtc acgaccgaga tgcccttcgc cggcccggtc
     1021 tccgggcggt cgagcgggac caccacggtg acgatggcgc gcttggtggc cgaggtcgac
     1081 ccatcgggtt cgtcgtcggc gtcggcggtc aggttgtcga gcagcaggag cgggaaggcg
     1141 accatcgtcg ccgccaccgc gacccacagg cgccgccgat caggcggtgc ggggttctcg
     1201 tcctccacgg ctctcctcca cgtcggacgg ggagaccgta cggaacccga ccgcgtacag
     1261 caaccttgat ggcacggtgc gtgccagaac cggcggcctc aggccagccg gtagcagtgc
     1321 tccgcgttgc gccaggtggc ctcggcgacg gcggcgaccg agtgcccgga ggcctcggcc
     1381 agaccggcac cgacgagggg cacccagccc ggctcgttcg tccggccccg cttggggacc
     1441 ggggccaggt acgggctgtc ggtctccacc agcatccgat cgaggggaca gcgggccgcc
     1501 gccgcccgca cgtcgtcggc gctcgggaac gtcacgatcc cggagaacga gaggaacgca
     1561 ccgcgggcca ggcactcgtc ggcctcgtcg gcgccgcccg tgaagcagtg gaacaccgtg
     1621 cgctcgggca cgccctcggc gtcgatgacc tcgaaggtct cctcccacgc ctcccgggtg
     1681 tggatcacga gggccttgtc gtgggcgtgg gccagggcga cctgggcggc gaagacgtcg
     1741 cgctggacgt cgcggggcga gtggtcgtag tggaagtcga agccgcactc ccccaccgcc
     1801 acgcacgacg ggtcggtcag gagctcctcg atgccgtcga cgccgtggcg ggcctcgtgg
     1861 gggtgcacgc cggcggtggc ccacaccccg tcgtgggccg cggcgagggc catggccgag
     1921 cgagacgaac ccaggtcggt gccgacgtgg accaaccgct cgaccccggc cgcccgcgcc
     1981 gcggcgagca cctcgcccac cgagtcgtcg tcgggcaggt ggcagtggtc gtcggtccag
     2041 cggagctcgc cgctcacggc ctcaggcctt gatccgaggg aagagcgggt cgcccttctc
     2101 gacagccagg ccgcccgggt actggcccca cgtggcggcg tccgggagcc gctggtcctc
     2161 cggcgtgccg ggcaggccga tgcggcgcca gatctcccgg cacgacgccg gcaccgccgg
     2221 agacgcgagg accgtgacga tccggagcgc ctcgagggcg tcgcccatga tccgctcgac
     2281 ctccgggccc gggtccatct tccagggctc gttggtctcg aggtgggcgt tggtctcctt
     2341 gatgagccgc caggtggcgt cgagggccac cgagggctga atgcggtccc aggccgccgc
     2401 agtgtccgcg taggtctccg cggcgactgc cgcgagcggg ctgtccgggt ccggggccgg
     2461 tccgatgcca tcgcacttct tcgcgaccac cgtcgcgacg cgcgacagga ggttgccgag
     2521 g
//
LOCUS       DATISU010000184         8296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_799974, whole genome shotgun sequence.
ACCESSION   DATISU010000184 DATISU010000000
VERSION     DATISU010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799974"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1599
                     /locus_tag="VLR27_07505"
     CDS             52..1599
                     /locus_tag="VLR27_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSP03328.1"
                     /translation="MSEHPTRPTTVTPNGSPLEQGTTIGGRYQLGSPIASGGMATVWR
                     ATDTVLARPVAVKVLHAHLAADPEFVARFRREAVAAARLAHPSIVAIYDTSDDPESIV
                     MELVDGRTLRDELDARGFLPPAEAVAVASSICQALEAAHKAGIVHRDVKPANVLLSAG
                     GQLKVADFGIAKAARTSGEDDLPDLTAIGRMVGTAKYLAPEQVSGGDIDARTDVYAVG
                     AVLYEMLCGRAPFTGDNDLAVATARLHGPPLRPRAVLRTVPPRLDEIVMKALATDPDE
                     RHHSASALWADLQSCPLDDQPLAPVVATDATVVGEQPSFTRAERSWLVPALLVSLVAV
                     ALGLAWVLIGGSEARRDLVDLVDRVRPGGETGAVDAPVDVLSVVAASDFDPQGDQGEH
                     PDEVPFALDGDPATAWTSENYDSRQDFLGLKDGVGLILDIGDSADVREVVLTTPNDGW
                     SAAIHVADSAGADIADWGEPVATVEGSGPGETTLELEAEGRYVLVWITDLGARSFELQ
                     EAVVRGR"
     gene            1658..2218
                     /locus_tag="VLR27_07510"
     CDS             1658..2218
                     /locus_tag="VLR27_07510"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP03329.1"
                     /translation="MAAAQGGDRAAMDALLRRHHERIWAVCRRLAGNDADAADATQEA
                     MLLIVRRIGTFDGRSRFTTWAHRVATNACLDELRRRKRRPTDHLPDDDPEHGRAIADR
                     SEPHDHAVSERMAIDDALASLPEEFRVPIVLRDLGGHDYAEIAAILDIPAGTVRSRIA
                     RGRARLAELLGPGNQASAPDRPSTRP"
     gene            2215..3093
                     /locus_tag="VLR27_07515"
     CDS             2215..3093
                     /locus_tag="VLR27_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03330.1"
                     /translation="MTDSPTPAEPGPVDPDVELASAHLDGEAGAEERDRVDDPAVQAH
                     RSAFGAVADQVRDVPPPPPGLLDDHVAAAMGAFDDDARVVPLAGRGSTRSWWQRIPFG
                     AVAAGLVIVALIVGIATTTDDDDDTDTAATALDSSGTEDAGDEALSDDGEASAGAGME
                     ESLESQEQFDTSGERMAYDSVDALVEDVRTRLPVGPDDPDAGAEAEADDTPVAESDPT
                     TTQTDRSVEDGDEGAVDPCDAVSLLGLDPADVVLVLPAVLTPDEVTVVVHDADDGRRL
                     TVVDDASCTTALDRLL"
     gene            complement(3140..3505)
                     /locus_tag="VLR27_07520"
     CDS             complement(3140..3505)
                     /locus_tag="VLR27_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03331.1"
                     /translation="MVTIRVLVAMALGALVAALGAVFLGEYEFDETLPIAAGALLGLV
                     VAEIVVSLGRHRSWTMAGILAVLVGVAIVLAGHLDAGDVEPVKAGAYLSAALGAGAAA
                     LRTAPWGRRTRSRAAQPGT"
     gene            3551..3904
                     /locus_tag="VLR27_07525"
     CDS             3551..3904
                     /locus_tag="VLR27_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03332.1"
                     /translation="MATTSPASPTPTPAAADQPDWPAQAADAIVEQVGKVRDRTTGPA
                     IKISGYVVFGAFATLLGTVAFILFLIGAFRVLDAYLPDAVFGDDHTWAAHTILGAILV
                     LAGLFTSRKMKGDNA"
     gene            3901..4920
                     /gene="trxB"
                     /locus_tag="VLR27_07530"
     CDS             3901..4920
                     /gene="trxB"
                     /locus_tag="VLR27_07530"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HSP03333.1"
                     /translation="MSDVRDVIIIGSGPAGLTAAIYTARASLDPLIIEGEPSSTSDQP
                     GGQLMLTTEVENFPGFPEGVMGPELMSSMREQALRFGADIRTEKVTKVDLSARPFGVW
                     IGDPDAAEPTYRAKTVIVSTGAQSLMLDLPRENELIGHGLSTCATCDGFFFRDQEIVV
                     VGGGDSAIEEATFLTKFASKVTIVHRRDELRASKIMQDRAFKNDKIEFLWDTTVEEYL
                     FDEGPLGAKLRGLRVRNVKTGEDSELLAGGVFIAIGHRPNTDLFKGQLAMEDNGYLIT
                     GASGTEMESATDVDGVFACGDVQDHTYRQAITAAGSGCMAAIDAERWLEAHDDIDQVR
                     TETTW"
     gene            5009..5329
                     /gene="trxA"
                     /locus_tag="VLR27_07535"
     CDS             5009..5329
                     /gene="trxA"
                     /locus_tag="VLR27_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HSP03334.1"
                     /translation="MAATTVTDATFDEVIGSADEAVIVDFWAEWCGPCKMIAPVLDEI
                     AAEQAGKVKVVKLNVDENQDTARRFDVMSIPTLIVFKDGQPAKKMIGAKGKGALLEDL
                     AEFL"
     gene            complement(5509..6450)
                     /locus_tag="VLR27_07540"
     CDS             complement(5509..6450)
                     /locus_tag="VLR27_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HSP03335.1"
                     /translation="MNAQQQPGGSMNRKSGLGRGLGALIPAEYSEAASNETMRGAFEV
                     PVAAVRPNPNQPRAHFDEESLAGLTASIAEIGVLQPVLVREVGEGQFELIAGERRWRA
                     SKRAGLQSIPVVVRDADTDASSLEQALVENLHRADLNAMEEAAAYQQLVEDFGLTQDA
                     VAKRVGKSRAAVSNTLRLFQLPPAIQALVAENQLTAGHARALLGTPDRAYQTALAQRI
                     VRDGLSVREAEEAVRLRSGDAPDPEPTPEPDSPQKPTQRPRPTSDPAFLELESLLGDH
                     LDTRVVISGGGGGKGRLTVEFADVADLERIYRRMVGG"
     gene            complement(6447..7559)
                     /locus_tag="VLR27_07545"
     CDS             complement(6447..7559)
                     /locus_tag="VLR27_07545"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSP03336.1"
                     /translation="MSDADPLSSRSDGIFERLRAAADEQDVHDAEQEADAVSADVSEG
                     GASTEPESEPVATPAPPAPRPVQMPDEARLEVGGLPGRDALPDLPVGTPQAGDAPTDR
                     RDAAGNALPRIMAVANQKGGVGKTTTTVNLAAALAEAGYRTLVVDLDPQGNASTGLGI
                     NIRDLQLSMYDVIIHEAPIDDTIEATAMKNLFVAPSSLDLAGAEIELVPAFSRELRLR
                     HALQSVADDYDYMLIDCPPSLGLLTVNGLAAAAEVLVPIQCEYYALEGLGQLLRNVSL
                     VQRNLNQSLVVSTFVCVMYDARTKLADQVVTEVRDHFGDKVCRIVIPRTVRLSEAPSY
                     GQPILAFDPRSRGAIAYRELAREVSGGPLETTSTTA"
BASE COUNT         1211 a         3125 c         2832 g         1128 t
ORIGIN      
        1 ccgcgccggc gactcgcggc ccggtgagcc ccgacgggta gcgtcggtgg ggtgtcggag
       61 caccccacgc ggccgacgac cgtgaccccg aacggcagtc ccctcgagca gggcaccacc
      121 atcggcggcc gctaccagct cggatctccg atcgcttccg gtggcatggc caccgtgtgg
      181 cgggccaccg acaccgtgct ggcccgcccg gtcgcggtga aggtcctgca cgcgcacctg
      241 gccgccgatc ccgagttcgt ggcccggttc cggcgcgagg ctgtcgccgc cgcccggctc
      301 gcccacccct cgatcgtggc catctacgac acctccgacg acccggagtc gatcgtcatg
      361 gagctggtcg acggccgcac gctgcgcgac gagctcgacg cccgcggctt cctgcccccc
      421 gccgaggccg tggcggtcgc gtcgtcgatc tgccaggcgc tcgaggccgc ccacaaggcc
      481 ggcatcgtcc accgcgacgt gaagccggcc aacgtgctgc tgtccgccgg cggccagctg
      541 aaggtggccg acttcggcat cgccaaggcg gcccgcacct cgggtgagga cgacctgccc
      601 gacctcactg ccatcggccg gatggtcggc accgccaagt acctcgcccc cgagcaggtg
      661 tccggcggcg acatcgatgc ccgcaccgac gtctacgcgg tcggggccgt gctctacgag
      721 atgctctgcg gtcgggcgcc cttcaccggc gacaacgacc tggccgtggc cacggcccgc
      781 ctgcacggcc cgccgctgcg gccccgcgcc gtgctgcgca ccgtgccgcc ccgcctcgac
      841 gagatcgtca tgaaggccct ggccaccgat cccgacgagc gccaccactc ggcctccgcg
      901 ctgtgggccg acctccagtc ctgcccgttg gacgaccagc cgctggcccc ggtggtggcc
      961 accgatgcca ccgtggtcgg cgagcagccc tccttcaccc gcgccgagcg gtcgtggctc
     1021 gtccccgccc tgctcgtgag cctggtcgcc gtcgccctcg gcctggcctg ggtgctcatc
     1081 ggcggcagcg aggcccgccg cgacctcgtc gacctggtcg accgcgtcag gccgggcggc
     1141 gagaccgggg ccgtggacgc cccggtcgac gtgctgtccg tcgtcgcggc gtcggacttc
     1201 gatccccagg gcgaccaggg cgagcacccc gacgaggtcc cgttcgccct cgacggcgat
     1261 ccggccaccg cctggaccag cgagaactac gactcccgcc aggacttcct cggcctgaag
     1321 gacggggtcg ggctcatcct cgacatcggc gacagcgccg acgtgcgcga ggtcgtgctc
     1381 accacaccca acgacgggtg gtccgccgcc atccacgtcg ccgacagcgc cggcgccgac
     1441 atcgccgact ggggcgagcc cgtggccacc gtggagggtt ccggtccggg cgagaccacg
     1501 ctcgagctcg aggccgaggg ccgctacgtc ctggtctgga tcaccgacct cggcgcccgc
     1561 agcttcgagc tgcaggaagc cgtggtccgc ggccgctgat cacggcggtc ggtacgctcc
     1621 cgccatcgct ggcgacaccc ggactgatga ggcgctggtg gccgccgccc agggcggtga
     1681 ccgggcggcg atggacgcgc tgctgcgtcg ccaccacgag cggatctggg ccgtgtgccg
     1741 gcgactcgcc ggcaacgacg cggacgccgc cgacgccacc caggaggcga tgctgctcat
     1801 cgtccggcgc atcggcacct tcgacggccg ctcccggttc acgacctggg cccaccgggt
     1861 cgcgaccaac gcctgcctcg acgagctgcg tcgccgcaag cgccgaccca ccgaccatct
     1921 gcccgacgac gaccccgagc acgggcgcgc catcgccgac cgcagcgaac cgcacgacca
     1981 cgcggtgagc gagcggatgg ccatcgacga cgccctcgcc tccctgcccg aggagttccg
     2041 ggtgccgatc gtgctgcgtg acctgggcgg gcacgactac gccgagatcg ccgccatcct
     2101 ggacatcccc gccggcaccg tccgctcccg catcgcccgg ggccgggccc gcctggccga
     2161 gctgctcggc ccggggaacc aggcgtccgc ccccgaccgt ccaagcaccc gaccatgacc
     2221 gactccccca cccccgccga gcccggcccc gtcgatcccg acgtggagct ggccagcgcc
     2281 cacctcgacg gcgaggcggg tgcggaggag cgcgaccggg tggacgaccc cgccgtccag
     2341 gcccaccgat ccgccttcgg ggcggtggcc gaccaggtgc gcgacgtccc tcctcccccg
     2401 ccgggcctcc tcgacgacca cgtggccgcg gcgatgggcg ccttcgacga cgatgcccgg
     2461 gtggtccccc tcgctgggcg gggctcgacc cggtcgtggt ggcagaggat cccgttcggc
     2521 gccgtcgcgg ccggtctcgt catcgtggcc ctgatcgtcg ggatcgccac caccaccgac
     2581 gacgacgacg acaccgacac cgcggccacc gcgctcgact cgagcggcac cgaggacgcc
     2641 ggcgacgagg cgctctcgga cgacggcgag gcctccgccg gcgccgggat ggaggagagc
     2701 ctcgagtccc aggagcagtt cgacaccagt ggcgagcgga tggcctacga cagcgtcgat
     2761 gcgctggtcg aggacgtccg cacccgactc ccggtcggcc cggacgaccc cgacgccggg
     2821 gccgaagccg aagccgacga cacccccgtc gccgagagcg acccgaccac gacccagacc
     2881 gaccgcagcg tcgaggacgg cgacgagggt gcggtcgacc cgtgcgacgc cgtgtcgctg
     2941 ctcggtctcg accctgccga cgtcgtgctg gtcctgccgg ccgtgctcac ccccgacgag
     3001 gtcaccgtgg tggtccacga cgccgacgac ggccgacgcc tcaccgtggt cgacgacgcc
     3061 agctgcacca ccgcgctcga ccgcctcctg tagacccgcc ccgccggaaa ccggcagtgc
     3121 ccctgttgcg gtgggagcgt caggtaccgg gctgggcggc ccggctgcgg gtgcgccggc
     3181 cccacggggc ggtccggagc gcggcggcac cggcgccgag ggctgcgctc aggtaggcgc
     3241 cggccttcac cggctccacg tcgccggcat cgaggtgccc ggcgagcacg atcgcgacgc
     3301 cgacgaggac ggcgaggatg cccgccatcg tccaggaccg gtgacgaccg agggagacga
     3361 cgatctcggc cacgaccagg ccgagcagcg cgccggcggc gatcggcagg gtctcgtcga
     3421 actcgtactc ccccaggaac accgccccca gagcagcgac gagggcaccg agagccatgg
     3481 ccacgagcac acggatggtc accacggggc acacggtagg tccccgcacg cccctgggta
     3541 gggtgctcgc atggccacga cctcaccggc gtcaccgacg cccacaccgg ccgccgccga
     3601 ccaaccggac tggcccgccc aggccgccga cgccatcgtc gagcaggtcg gcaaggtccg
     3661 cgaccgcacc accggtccgg ccatcaagat ctcgggctac gtcgtcttcg gggccttcgc
     3721 gacgctcctc ggcacggtgg cgttcatcct gttcctcatc ggcgccttcc gcgtcctcga
     3781 cgcctacctg cccgatgccg tgttcggcga cgaccacacc tgggccgcgc acacgatcct
     3841 cggggccatc ctggtcctcg caggcctgtt caccagccgc aagatgaagg gcgacaacgc
     3901 atgagcgacg ttcgcgacgt gatcatcatc ggttcgggcc ccgccgggct caccgccgcc
     3961 atctacaccg cccgggccag cctcgacccc ctgatcatcg agggcgagcc ctcctccacc
     4021 agcgaccagc ccggcggcca gctgatgctc accaccgagg tggagaactt ccccggcttc
     4081 cccgagggcg tcatgggtcc cgagctcatg tcgagcatgc gcgagcaggc cctgcgcttc
     4141 ggggccgaca tccgcaccga gaaggtgacc aaggtcgacc tgtcggcccg cccgttcggc
     4201 gtgtggatcg gcgaccccga cgccgccgag ccgacctacc gggccaagac ggtcatcgtg
     4261 tccaccggcg cccagtcgct catgctggac ctgccccggg agaacgagct catcgggcac
     4321 gggctgtcga cctgcgccac ctgcgacggc ttcttcttcc gagaccagga gatcgtggtc
     4381 gtcggcggcg gcgactccgc catcgaggag gccacgttcc tgaccaagtt cgcctccaag
     4441 gtcacgatcg tgcaccgccg cgacgagctg cgcgcctcca agatcatgca ggaccgggcg
     4501 ttcaagaacg acaagatcga gttcctgtgg gacaccaccg tcgaggagta cctcttcgac
     4561 gagggtcccc tcggcgccaa gctccgcggc ctgcgggtcc gcaacgtcaa gacgggcgag
     4621 gactccgagc tgctcgccgg cggcgtgttc atcgccatcg ggcaccggcc caacaccgac
     4681 ctgttcaagg gtcagctggc catggaggac aacggctacc tcatcaccgg ggcgtccggc
     4741 accgagatgg agtcggccac cgacgtcgac ggcgtgttcg cctgcggcga cgtccaggac
     4801 cacacctacc ggcaggccat cacggctgcg gggtcgggtt gcatggccgc catcgacgcc
     4861 gagcgctggc tcgaggccca cgacgacatc gaccaggtcc gcaccgagac cacctggtag
     4921 gtcccggctc gaccggggat ggaatgccag cggctccccc tcggttcaac acagctgcat
     4981 cccccgatca cgaaggaagt tctgcaccat ggctgcaacc accgtcaccg acgccacgtt
     5041 cgatgaggtc atcggctccg ccgacgaggc cgtcatcgtc gacttctggg ccgagtggtg
     5101 cggtccgtgc aagatgatcg cccccgtgct cgacgagatc gccgccgagc aggccggcaa
     5161 ggtcaaggtc gtcaagctca acgtcgacga gaaccaggac accgcccgcc gcttcgacgt
     5221 catgtcgatc cccaccctca tcgtcttcaa ggacggccag ccggccaaga agatgatcgg
     5281 cgcgaagggc aagggcgccc tgctcgagga tctcgccgag ttcctgtagg ccgcgaatgc
     5341 cctggtcaca gggctagtgc cgttggttgt gccacttcct agcgcggatg gaccagtcac
     5401 ctcgtgtgac gtgggtcacc ctcgaccgca ggttttccac aggcactcca caactgggga
     5461 caccggtcca gttggacctg agggtcgacc tcgaccctcg atgcggcctc agccgcccac
     5521 catgcggcgg tagatgcgct ccaggtcggc gacgtcggcg aactccaccg tcaggcggcc
     5581 cttgccgccc ccgccgccgg agatcaccac gcgtgtgtcc aggtggtccc cgaggagcga
     5641 ctcgagctcc aagaacgcgg ggtccgaggt ggggcgcggc cgctgtgtgg gcttctgggg
     5701 cgaatcgggc tcgggcgtgg gctccgggtc cggggcgtcc cccgaacgca acctgacggc
     5761 ttcctcggcc tctcgcaccg acaggccgtc gcgcacgatc cgctgagcga gcgccgtctg
     5821 gtaggcgcgg tcgggcgtgc cgagcagggc gcgggcatgg ccggcggtga gctggttctc
     5881 ggccacgagg gcctggatgg ccggcggcag ctggaacagg cgcagcgtgt tgctcaccgc
     5941 ggctcggctc ttgccgaccc gcttggccac ggcatcctgg gtgaggccga agtcctccac
     6001 cagctgctgg taggcagctg cctcctccat ggcgttgagg tcggcgcggt ggagattctc
     6061 gaccagtgcc tgctcgagcg acgacgcatc ggtgtccgcg tcgcggacga ccaccgggat
     6121 ggactgcagc cccgctcgct tcgaggcccg ccaccggcgc tcgccggcga tgagctcgaa
     6181 ctggccctcc cccacctcac gcaccagcac cggctggagc acgccgatct ccgcgatgga
     6241 ggcggtcagc ccggcgagcg actcctcgtc gaagtgggcc cgaggctggt tcgggttcgg
     6301 ccgcaccgcg gccacgggga cctcgaaggc acctcgcatc gtctcgttcg acgcggcttc
     6361 gctgtactcc gcggggatca gggcccccag gccccgtccc agtcccgact tgcggttcat
     6421 cgatccccct ggttgctgct gggcgttcat gcggtggtgc tggtcgtctc gagcgggccg
     6481 ccgctgacct cacgggccag ctcccggtag gcgatggcgc cgcgcgagcg cgggtcgaag
     6541 gccaggatgg gctggccgta ggacggggcc tcggacaggc ggacggtgcg tgggatcacg
     6601 atgcgacaca ccttgtcccc gaagtggtcc cgtacctcgg tgaccacctg gtcggccagc
     6661 ttggtccggg cgtcgtacat cacgcagacg aaggtggaca cgaccagcga ctggttgagg
     6721 ttgcgctgga ccagcgacac gttgcgcagc agctggccga ggccttccag ggcgtagtac
     6781 tcgcactgga tcggcaccag cacctcggcc gccgcggcca ggccgttcac cgtcagcagc
     6841 ccgagcgacg gtgggcagtc gatgagcatg tagtcgtagt cgtcggccac cgattgcagg
     6901 gcgtggcgca gccgcaactc ccgggagaag gcggggacca gctcgatctc ggcaccggcc
     6961 aggtcgaggc tggacggggc cacgaacagg ttcttcatcg ccgtggcctc gatggtgtcg
     7021 tcgatcggcg cctcgtggat gatcacgtcg tacatcgaca gctgcaggtc ccggatgttg
     7081 atcccgagac cagtgctggc gttgccctgc ggatcgaggt ccacgaccag ggtccggtac
     7141 ccggcttcgg ccagcgccgc ggccaggttc accgtcgtgg tcgtcttgcc cacgccaccc
     7201 ttctggttgg ccacggccat gatgcggggc agggcgttcc cggcggcgtc ccgacgatcc
     7261 gtcggcgcat cgccggcctg cggggtgccg accggcagat cgggcagggc gtctcggccc
     7321 ggaaggccgc cgacctcgag gcgggcctcg tcgggcatct gcaccgggcg gggggccggg
     7381 ggggccgggg tcgcgaccgg ctccgactcc ggctcggtgc tggcgccacc ttcgctgacg
     7441 tcggccgaca ccgcgtccgc ctcctgctcg gcgtcgtgga catcctgttc gtccgcagca
     7501 gcacggaggc gctcgaagat gccatcactg cgggacgaga gcggatcggc atcagacacc
     7561 gacccatggt gcttgccagc cgcggggcgg gtcaaggacc gcacgatgtt tcacgtgaag
     7621 cacttgaggg tttcggcggg gttcggcaat cgcaacgagt cggcccggcg cttcggtctc
     7681 ccggaggtcc cgaccgttcg accagggtgc ccactggcga cgcgtccgcg gtgaaccggg
     7741 tcggcaggga gccgcggaac gttccacgtg aaacattttc gctcgtgcgt gcgggactgc
     7801 ttcacgtgaa acaccccgaa gtatcggtgg gctgcggctg tgccgcctcc gcccccagaa
     7861 acaccgccgc caccggggac ccggccggat gcacgaggac agcccagcgg ccaccggcga
     7921 gacgttgggg cgatccggca ccgtgtccca cccgccttcc ggggccacgc cccgacgcgg
     7981 ggcgccgttc gtggcgaacc tgcgaccctc gcttcgccac cttcgcccgg accggccggg
     8041 tcgggcccgg ggacctcaga gaccgccaca tcgcacccgg aagcggtcgc cggcccggtg
     8101 gatccgtgca gcggacaacc gcgacgttgc gccgccgctt gatcgagcgg actctgacgg
     8161 cagcaagccc taccggctcc gatgtttcac gtggaacatc gctcagcgac gggccaggag
     8221 ggactcgacc cgagaagtca cgaggtccac cccgacgacg ttctccacgc cccgtgggac
     8281 gatcacgtcg gcccag
//
LOCUS       DATISU010000185         4056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_800053, whole genome shotgun sequence.
ACCESSION   DATISU010000185 DATISU010000000
VERSION     DATISU010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800053"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VLR27_07550"
     CDS             <1..367
                     /locus_tag="VLR27_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HSP03337.1"
                     /translation="VSTCVGIGGDPVPGTTFIDCIERFEADPETQAIMMIGEIGGSAE
                     EEAADYIKANVSKPVVSYVAGVTAPPGKKMGHAGAIVSGGKGTAAAKREALEAAGVKV
                     GNNPTEAGELMVEVVNGLR"
     gene            495..1523
                     /locus_tag="VLR27_07555"
     CDS             495..1523
                     /locus_tag="VLR27_07555"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HSP03338.1"
                     /translation="MPRSSRLILFSAIVLLAAGVVAALAAASEGDPVTGPVTGGPAET
                     SSGVSAGPASTAPATTAVLGFPAIADHGEVRAVRTPAGFVLPVLGGQEGAWEVVTPCA
                     ASAVVPGEPVLGAHVVLDPGHGGREPGAVGPSGLREADVNLDVARRVRTLLEAEGATV
                     VLTRDADVRMTLSTRASIATAMAPVAFLSIHHNAAPVGRSDVPGSELYHQLADPESKR
                     LAGLLWEEMQEHLAPFGTDWAIGDDPGARARRSQRTGDDYYGILRHAQGIPAVLTEAA
                     FLSNPAENALLLTDEFRDAEARAIADGVLRLLRTDDPGSGFKPTKEVAVPAGSGGGSS
                     GCVDPPLT"
     gene            1601..2413
                     /locus_tag="VLR27_07560"
     CDS             1601..2413
                     /locus_tag="VLR27_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HSP03339.1"
                     /translation="MSTPCTTCGEPLADDALRCPTCGALASPGVDETRAVDQTRSLDD
                     APPWAIPIREPAPAPAAASEARTPAGRTIALAALAIGVFVLTAMIGTRLLGDDDSSAS
                     SRDAGSDEAASPDETGGLDGDVVAETTSTTTSTSTPSTTETTVADTTTTTSTPTTSAA
                     PTTTVDAFRTAPNGTGSVPALSTSFRGWLAQLKSVPYDAGTAALAEEWERTRADAPRV
                     VAARSSDWSAFGEGFWVLVDPGPFSSPDEVRSFCESAGFTADGACLVRELRG"
     gene            2462..3064
                     /gene="purN"
                     /locus_tag="VLR27_07565"
     CDS             2462..3064
                     /gene="purN"
                     /locus_tag="VLR27_07565"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HSP03340.1"
                     /translation="MRIAVLASGSGTLLDAILTDGIPVELVVVDRPCAATDVAERHGV
                     RAVCHARTSFGPDFDREAYTAEVTEVLVDAGIDLVVMAGYGTVFGRAIHDAFPHRILN
                     THPALLPAFKGWHAVDAALDYGVKVTGCTVHVATLEMDAGPILAQEAVPVLADDTADS
                     LHDRIKQVERRLYPDTIRVVLADPAILDRSPRTSATEESA"
     gene            3061..>4056
                     /locus_tag="VLR27_07570"
     CDS             3061..>4056
                     /locus_tag="VLR27_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase PurH"
                     /protein_id="HSP03341.1"
                     /translation="MRAFLSVYDKTGVVDLARGLVDAGWQLVSSGGTAAAIRDAGLDV
                     TSTEEVTGYPAILGHRVATLHPKIHGAILADPSDPEHQADLETYEIEPFSLVVSNLYP
                     FGQPDAAGDAGGRRLAEGVDLIDIGGPAMVRAAAKNHANVGIIVDPADYDVVLAEIRR
                     DGELGDTTRWRLARKAFAHTAAYDAAVVSWFDESAPEDSDEADALPVTVHLALERVDE
                     LRYGENPHQRGARYREIGRSSWMDGITQHSGLPLSYLNLFDADAAWSLAHDLAEVSPG
                     KAAVAIIKHANPCGAAVADTLEEAYRLAFECDPRSAFGGIVATSQPIDDATVERMV"
BASE COUNT          546 a         1614 c         1359 g          537 t
ORIGIN      
        1 cgtgtcgacc tgcgtcggca tcggcggcga ccccgtcccc ggcaccacct tcatcgactg
       61 catcgagcgc ttcgaggccg accccgagac ccaggcgatc atgatgatcg gcgagatcgg
      121 cggctcggcc gaggaggagg cggcggacta catcaaggcc aacgtctcca agccggtcgt
      181 gtcctacgtc gccggcgtca ccgcccctcc cggcaagaag atgggccacg ccggcgccat
      241 cgtgtcgggc ggcaagggca ccgccgccgc caagcgcgag gccctcgagg ccgccggcgt
      301 caaggtgggc aacaacccca ccgaggccgg cgagctcatg gtcgaggtgg tcaacgggct
      361 gcgctgagcc cacctccagc agctcttcgt tctggcctgc atccgtgcca cctggtggtc
      421 atggatgcag gccagaaccg tttccgggcg gctggagcgt tggggtaccg caccgccgcc
      481 tccatccttg ggggatgccc cgttcgtccc gcctgatcct gttcagcgcg atcgtgctcc
      541 tggcggccgg ggtcgtcgcc gcgctggccg cggcgagcga gggcgatccc gtcaccggcc
      601 cggtcacggg tggacctgcg gagacgtcgt ccggcgtctc cgccggcccc gcctccacgg
      661 cgccggccac caccgccgtg ctcgggttcc ccgccatcgc cgaccacggc gaggtccggg
      721 ccgtgcgcac gccggcgggc ttcgtcctgc ccgtcctcgg cggccaggag ggggcatggg
      781 aggtcgtcac cccgtgcgcg gcctcggcgg tcgtgccggg cgagccggtg ctcggcgccc
      841 acgtcgtgct cgaccccggc cacggtggcc gggagcccgg cgccgttggc ccgtccggtc
      901 tgcgcgaggc cgacgtcaac ctcgacgtcg cccgccgcgt ccggaccctg ctcgaggccg
      961 agggcgccac cgtcgtgctc acccgcgacg ccgacgtgcg catgacgctg tccacccggg
     1021 cgtcgatcgc cacggcgatg gcacccgtcg cgttcctctc gatccaccac aacgctgccc
     1081 ccgtcgggcg ctccgacgtc cccggcagcg agctgtacca ccagctcgcc gaccccgagt
     1141 ccaagcgcct ggccgggctc ctgtgggagg agatgcagga gcacctcgcc ccgttcggca
     1201 ccgactgggc catcggcgac gatcccggcg cccgggctcg acgctcgcag cgcaccgggg
     1261 acgactacta cggcatcctc cgccacgccc aggggatccc ggccgtgctg accgaggcgg
     1321 cgttcctgtc gaacccggcg gagaacgccc tgctgctgac cgacgagttc cgcgacgccg
     1381 aggcccgggc catcgctgac ggcgtcctgc gactgctgcg gacggacgac cccggctccg
     1441 ggttcaagcc gaccaaggag gtcgcggtgc cggccggctc cggtggcggc agcagcggct
     1501 gcgtcgaccc acccctcacc tgagcagggg tccaacggcg gccgcacccc gtcgtggcac
     1561 catggtcggg gcggtcggag cgatgaggat ccgcgcgccc atgagcaccc cctgcaccac
     1621 ttgtggcgaa ccgctcgccg acgacgccct ccgttgcccg acctgtggcg ccctcgcctc
     1681 gcccggtgtc gacgagaccc gggcggtcga ccagacccgc tccctcgacg acgcaccgcc
     1741 gtgggcgatc ccgatccggg agcccgctcc ggccccggcc gctgcgtcgg aagcccgcac
     1801 cccggccggt cgcacgatcg cgctggctgc gctcgccatc ggcgtgttcg tcctcaccgc
     1861 catgatcggc acccggctgc tgggcgacga cgactcgagc gcctcctccc gcgacgccgg
     1921 cagtgacgag gccgcctcgc ccgacgagac cggcgggctc gacggcgacg tggtcgccga
     1981 gacgacgagc accaccacca gcacctcgac gccgagcacc accgagacga cggtcgccga
     2041 cacgaccacc acgaccagca cccccacgac ctcggcggcg ccgaccacca ccgtcgacgc
     2101 gttccggacc gcaccgaacg gcaccggctc cgtgccggcc ctgtccacca gcttccgggg
     2161 ctggctcgct cagctgaagt cggtgcccta cgacgccggc accgcagcat tggccgagga
     2221 gtgggagcgc acccgcgccg acgccccgcg tgtcgtggcg gcgcggagca gcgactggtc
     2281 ggccttcggc gagggcttct gggtgctcgt ggacccgggt ccgttctcct cacccgacga
     2341 ggtccgctcg ttctgcgaat ccgccgggtt caccgccgac ggcgcctgcc tggtccggga
     2401 gctccgcggc tgaccagggc gtgcggggga tcgggggacc gcttgctacc ttccgcccct
     2461 catgcgcatc gccgtgctcg cctccggcag cgggaccctc ctcgacgcga tcctcaccga
     2521 cggcatcccc gtcgagctgg tggtggtcga ccgcccctgc gcggccaccg atgtcgccga
     2581 gcgccacggg gtgcgtgcgg tgtgccacgc ccgcacctcc ttcggccccg acttcgatcg
     2641 tgaggcctac accgccgagg tcaccgaggt gctggtggac gccggcatcg acctggtcgt
     2701 gatggccggc tacggcaccg tgttcgggcg cgccatccac gacgcgttcc cgcaccgcat
     2761 cctcaacacg cacccggcgc tgctgcccgc gttcaagggc tggcacgccg tcgacgccgc
     2821 cctcgactac ggggtgaagg tcaccggctg cacggtgcac gtggccacgc tcgagatgga
     2881 cgccggcccg atcctggccc aggaggccgt gcccgtcctg gccgacgaca ccgccgactc
     2941 gctccacgat cgcatcaagc aggtcgagcg tcgcctgtac cccgacacca tccgcgtcgt
     3001 gctggccgat cccgccatcc tcgaccggtc cccgcgaacg agtgcaaccg aggagtccgc
     3061 atgagggcgt tcctgtccgt ctacgacaag accggcgtgg tcgacctcgc ccggggcctg
     3121 gtcgacgccg gttggcagct cgtgtccagc ggcgggacgg ctgcggccat ccgcgacgcc
     3181 ggcctcgacg tcaccagcac cgaggaggtc accgggtacc cggccatcct cgggcaccgg
     3241 gtcgccaccc tgcacccgaa gatccacggg gcgatcctcg ccgatccctc cgatccggag
     3301 caccaggccg acctcgagac ctacgagatc gagccgttct ccctcgtggt ctcgaacctc
     3361 tacccgttcg ggcagcccga cgcggccggt gacgccggcg gccgtcgcct ggctgagggc
     3421 gtcgacctca tcgacatcgg tggcccggcc atggtgcgag cggcggcgaa gaaccacgcc
     3481 aacgtcggca tcatcgtgga ccccgccgac tacgacgtcg tgctcgccga gatccgccgt
     3541 gacggcgagc tgggcgacac cacccgctgg cgcctggccc gcaaggcctt cgcccacacc
     3601 gcggcctacg acgccgccgt cgtgtcctgg ttcgacgagt ccgcgcccga ggacagcgac
     3661 gaggccgatg ccctgcccgt caccgtgcac ctcgccctcg agcgggtgga cgagctgcgc
     3721 tacggcgaga acccgcacca gcgcggcgcc cgctaccgcg agatcggccg gtcgtcgtgg
     3781 atggacggca tcacccagca ctccggcctg ccgctcagct acctcaacct cttcgacgcc
     3841 gacgcagcgt ggagcctggc ccacgacctg gccgaggtct cgcctggcaa ggccgccgtg
     3901 gccatcatca agcacgccaa cccgtgcggc gctgccgtgg ccgacacgct ggaggaggcc
     3961 taccgcctcg ccttcgagtg cgacccgcgc tcggccttcg gcggcatcgt cgccaccagc
     4021 cagcccatcg acgacgccac ggtcgagcgg atggtc
//
LOCUS       DATISU010000186         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_614462, whole genome shotgun sequence.
ACCESSION   DATISU010000186 DATISU010000000
VERSION     DATISU010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614462"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VLR27_07575"
     CDS             <1..189
                     /locus_tag="VLR27_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2"
                     /protein_id="HSP03342.1"
                     /translation="RCLQKWPDAIVAANWDSLVFDVGGDPLRRVPMMEPSRGTAEHVG
                     TLIDECETAAELLSRLGS"
     gene            227..421
                     /locus_tag="VLR27_07580"
     CDS             227..421
                     /locus_tag="VLR27_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HSP03343.1"
                     /translation="MAEREQKKKSAPQRDEETVEEVPAGSESGEKIKAELDDLLDEID
                     DVLEQNAEDFVKSYIQKGGE"
     gene            443..1288
                     /gene="prcB"
                     /locus_tag="VLR27_07585"
     CDS             443..1288
                     /gene="prcB"
                     /locus_tag="VLR27_07585"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HSP03344.1"
                     /translation="MTLPFFTPDADPGPDFAGLLRRQGRDPETRIADAASHPLTIMHG
                     TTVVAIRTAGGVVMAGDRRATSGNIISHRSMEKVFPADRFSGVAIAGAAGPAMEMVRL
                     FQLQLEHYEKVEGHHLSLEGKANQLSQMVRAHLPAAMQGMVVVPLFAGYDLQRGVGRL
                     FEFDVTGGRYEELHHAATGSGSLHAGTVVKIGYRAEMDPSDAVDLAIRALFEAADDDS
                     ATGGPDLVRGIFPIVAVIDSDGYRRLEDDEIRRRFEGLLGDLSPSNTQGSADGGARPV
                     QEVER"
     gene            1285..1962
                     /gene="prcA"
                     /locus_tag="VLR27_07590"
     CDS             1285..1962
                     /gene="prcA"
                     /locus_tag="VLR27_07590"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HSP03345.1"
                     /translation="MSMPFYVAPEQVMKDRADYARKGIAKGRSLCAVSYDDGILFAAE
                     NTSNTLRKISEIYDRIAFAGVGRYSEFDQLRVAGVRHADMKGYAYSREDVDAQSLANA
                     YAQMLNHTFTHEMKPMEVEILVAEIGDERSGDELFHILYDGSVVDEDRFSVLGGEADA
                     ITARLDEAWADDLDLGGALRAAVAALAGDDRTLGADDLEIAVLARSNGRRKFARIHGE
                     ALTALLG"
     gene            2086..2532
                     /locus_tag="VLR27_07595"
     CDS             2086..2532
                     /locus_tag="VLR27_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSP03346.1"
                     /translation="MVGTRPGAGADDPVTGPMPAITLHDGRMQVHVRRDVTIVTLDGG
                     LDGAFAEDLAPTIPGALAGAEAVVLDVDQVTLLDQAGFDILAEAFGKATAGLDCCIVA
                     SRLSGRMVLERWGVTEHLAVFTSVADALQARAFADNGYGDGWQPEG"
     gene            2660..>2905
                     /locus_tag="VLR27_07600"
     CDS             2660..>2905
                     /locus_tag="VLR27_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HSP03347.1"
                     /translation="MERRIFGLENEYGVTCTLRGQRRLSPDEVARYLFRRVVSWGRSS
                     NVFLANGARLYLDVGSHPEYATPECDDLVDLVTHDKAG"
BASE COUNT          463 a         1052 c          986 g          404 t
ORIGIN      
        1 cggtgcctgc agaagtggcc cgacgccatc gtggccgcga actgggactc gctggtgttc
       61 gacgtgggcg gcgacccgct gcggcgggtg ccgatgatgg agccgtcgcg tggaaccgcc
      121 gagcacgtgg gtacattgat cgatgaatgc gagacggcgg ccgagctgct gtcccgtttg
      181 ggttcgtgac accgcgtcac ggacgggcaa gaccctggag ctcgtgatgg ctgaacgaga
      241 gcagaagaag aagtccgccc cgcagcgcga cgaggagacc gtcgaagagg tcccggccgg
      301 gtcggagtcc ggcgagaaga tcaaggccga gctggacgac ctgctggacg agatcgacga
      361 cgtgctcgag cagaacgccg aggacttcgt gaagtcctac atccagaagg gcggcgagta
      421 gcgcggcggt agtgtccgct acgtgacact gccgttcttc actcccgatg ctgatccggg
      481 tcctgacttc gccgggctgc tgcgccgcca ggggcgagac cccgagaccc gcatcgccga
      541 cgctgccagc cacccgctga cgatcatgca cggcaccacc gtcgtggcga tccgcaccgc
      601 cggtggcgtg gtcatggccg gcgaccgtcg ggccacgtcc ggcaacatca tctcgcaccg
      661 ctcgatggag aaggtgttcc cggccgaccg cttctcgggc gtggccatcg ccggcgccgc
      721 tggtccggcc atggagatgg tgcggctgtt ccagctccag ctcgagcact acgagaaggt
      781 ggagggccac cacctcagcc tcgagggcaa ggccaaccag ctcagccaga tggtgcgggc
      841 ccacctgccc gccgccatgc aggggatggt cgtcgtgccc ctcttcgccg gctacgacct
      901 ccagcggggc gtcggccggc tcttcgagtt cgacgtcacc ggtggccgct acgaggagct
      961 gcaccacgcc gccaccggca gcggttccct gcacgccggc accgtcgtga agatcgggta
     1021 ccgcgccgag atggacccat ccgacgccgt cgacctcgcc atccgcgccc tgttcgaggc
     1081 cgccgacgac gattccgcca ccggcggacc cgacctggtc cgcggcatct tcccgatcgt
     1141 ggccgtcatc gacagcgacg ggtaccgccg cctcgaggac gacgagatcc ggcgccgctt
     1201 cgagggcctc ctcggcgacc tgtcgccctc caacacccag gggtccgccg acggcggcgc
     1261 ccgcccggtc caggaggtcg agcgatgagc atgcccttct acgtcgcccc cgagcaggtg
     1321 atgaaggacc gggccgacta cgcccggaag gggatcgcca agggccgcag cctgtgcgcc
     1381 gtctcctacg acgacggcat cctcttcgcc gccgagaaca cctccaacac gctgcgcaag
     1441 atctccgaga tctacgaccg catcgcgttc gccggcgtcg gccgctacag cgagttcgac
     1501 cagctgcggg tcgccggcgt ccgccacgcc gacatgaagg gctacgccta ctcccgcgag
     1561 gacgtcgacg cccagagcct ggccaacgcc tacgcccaga tgctgaacca caccttcacc
     1621 cacgagatga agccgatgga ggtggagatc ctcgtggccg agatcggcga cgagcgcagc
     1681 ggcgacgagc tgttccacat cctctacgac ggctcggtcg tggacgagga ccgcttctcg
     1741 gtgctcggcg gcgaggccga cgccatcacg gcgcggttgg acgaggcctg ggccgacgac
     1801 ctcgacctgg gtggcgcgct gcgcgccgcc gtcgccgccc tggccggcga cgaccgcacc
     1861 ctcggggccg acgacctcga gatcgccgtg ctggcccgct cgaacggtcg ccgcaagttc
     1921 gctcggatcc acggcgaggc gctcaccgca ctgctcggct gaccgccgag gtcaggaaat
     1981 gcagttgaca gtcacgtgac ggcctgtaac ctgatcgcaa cagacccgga gcctcccgtg
     2041 accatcgccg aacgcgcccc cccccactcc gacatggatc ccagcatggt cggcacgcgt
     2101 cccggcgcag gagcggacga cccggtcacc gggccgatgc ccgccatcac gctccacgac
     2161 gggcgcatgc aggtgcacgt gcgacgcgac gtcacgatcg tgaccctcga cggcggcctc
     2221 gacggggcct tcgccgagga cctcgcgccc accatccccg gtgcgctcgc cggcgccgag
     2281 gcggtcgtcc tcgacgtgga ccaggtcacc ctcctcgacc aggcgggctt cgacatcctg
     2341 gccgaggcgt tcgggaaggc gacggccggg ctcgactgct gcatcgtggc atcgcgcctg
     2401 tcgggtcgca tggtgctcga gcggtggggc gtgaccgagc acctcgccgt gttcacgagc
     2461 gttgccgatg cgctccaggc ccgtgccttc gccgacaacg gctacggcga cggctggcaa
     2521 ccggagggct gaggagccga gccgacaggc gaggcgaccc atgagcacgg gccctgtggc
     2581 ggcgaagccg tcacgaccga accgcgggag ggcgtgacct gtccgtgcac gttccagacc
     2641 cgccgatagg ttgcggtgcg tggagcgccg catcttcgga ctcgagaacg aatacggcgt
     2701 cacctgcacc ctgcgggggc agcggcggct gagtcccgac gaggtcgccc ggtacctgtt
     2761 ccgccgggtc gtgtcctggg gccggtcctc caacgtgttc ctggccaacg gcgcccgcct
     2821 ctacctcgac gtgggctccc accccgagta cgccacgccc gagtgcgacg acctcgtcga
     2881 cctggtgacc cacgacaagg cgggc
//
LOCUS       DATISU010000187         6729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_521426, whole genome shotgun sequence.
ACCESSION   DATISU010000187 DATISU010000000
VERSION     DATISU010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521426"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="VLR27_07605"
     CDS             complement(<1..1493)
                     /locus_tag="VLR27_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HSP03348.1"
                     /translation="MLTASGLSLAHGSRVLFEDVTLSLSPGRRVAMVGGNGQGKTSLL
                     SILVGDAEPDTGSVTKPKDMRIGYLAQEIPSEDDGTVLDTVLGGRPDIMRLHHQIVEG
                     PADASPEALHAFGEAQTQYEQLNGYALEAEAHSVLAGLGFAPDDADRQVSELSGGWRM
                     RVALARLLLSSPDLLVLDEPTNHLDVDSVGWLEARLRAWTGALLFVSHDRDFIDNVAT
                     HVVELANQTLTEYVGGFAEFVVQREERIAQLEAQAASQAKARAQTERFVERFRYKATK
                     ARQVQSRIKMLEKLEKIEIPDRKELQARFQFPEPPRTSRVVAELSHMTAGYGDTPILR
                     DVSVHVERGQKIALVGPNGAGKTTLLKMLLGDLPPMAGTAEVGQSVTVARFEQHQADE
                     LAPNQTVLQVARDGIGSDTPRGKSLRTYLGSFGFRDDAVERQVGMLSGGERTRLALAK
                     AMAEPAGLLILDEPTNHLDLASCDMLEDALKVYPGTVLLVTHDRYLIR"
     gene            1578..2525
                     /locus_tag="VLR27_07610"
     CDS             1578..2525
                     /locus_tag="VLR27_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP03349.1"
                     /translation="MADGPLEPDALVRARGLTKRFGDFVAVDGIDFEVRRGEVFGFLG
                     PNGAGKTSTMRMISCVSPSTGGELSVLHLDPSADGPEIRGRIGVVPQEDNLDTELSVW
                     DNFIVYGRYFGLPRRVLRERAQELLEFARLTDRRDDRVDPLSGGMKRRLTIARSLIND
                     PDLVLLDEPTTGLDPQARHLLWDRLYGLKRRGVTLIITTHYMDEAEQLCDRLVVMDQG
                     RIVDEGSPRELIHRHVTPEVIELRFAEPAEQEAALSSAGEAAERIEVLADRLLLYTRD
                     AEATHRALAERSVEPLTELTRRASLEDVFLALTGRTLEE"
     gene            2536..3306
                     /locus_tag="VLR27_07615"
     CDS             2536..3306
                     /locus_tag="VLR27_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP03350.1"
                     /translation="MTQSVWRALERELLVFARTWRGNAFTAIVQPVLFLLAMGIGLGG
                     LVDEGAGTASVQLDYLEFVAPGLLAASALLAVAGEALWGVMGGVKWMGQFKGMVATPM
                     TPGDVYGGLVLSAGVRAGAAAVAFLVVAAVLGGVPSLWAPLAIVAAVLLAIVTMSALS
                     AYAVRKEDDRTFPLIMRLGVIPMFLFSGTFFPVEQLPDVVQPIAVLSPLWHGVEVARD
                     ATTGAVDGWTLLHVGVLVALAAAALPFGVRGFERRLTP"
     gene            3303..4115
                     /locus_tag="VLR27_07620"
     CDS             3303..4115
                     /locus_tag="VLR27_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP03351.1"
                     /translation="MSVQDLVDHGRIAPPIDARKRFLRLTERNVVGYMRAWPILLTGL
                     VEPFLYLLSIGIGVGALVGDITYAGRQVPYETFVASGMLATAAMNGAVFDTTFNFFIR
                     LRYAKLYDAVLATPLEPRDVALGEVTWALMRGTFYSAAFLVTMALFGLTESWWAIAAL
                     PATVLIGAAFASVGLAGATFLRSFLDFDFVNLAITPMFLFSATFFPLSQYPDALAAVI
                     RYTPLYQGVALCRGLTTGEVGFGLLVHVAYLLGMVVVGVAVASRRIDGLLRS"
     gene            4196..4867
                     /locus_tag="VLR27_07625"
     CDS             4196..4867
                     /locus_tag="VLR27_07625"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSP03352.1"
                     /translation="MIHEIDIDLPVNTVYNQWTQFEDFPLFMKHVVDVRQIDDTHTAW
                     KAKISGISREWQAEITEQTPDQRVAWTATDGTENSGVVTFHALDDNTTRVVLQMETDP
                     KGFLEKVADWGGYISDRSKKDLEQFKDFIEARGKSTGAWRGQVERDSMRDLHSREDEL
                     RQLSDHELAKRAEDAGLERPLDRNRDWLVSALARDGYERRTGRDRGGEGDHDDRRDPE
                     DAHTS"
     gene            complement(4889..5491)
                     /locus_tag="VLR27_07630"
     CDS             complement(4889..5491)
                     /locus_tag="VLR27_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP03353.1"
                     /translation="MGDEPAAWMAAGFTVEGAEVAVGTVRISCTGDGAGADRWRLRAD
                     GDVPRSVDGIATSTTDREPPRPVAHPNGVVRIDHIVLRSPDLDRTTEAFGALGLDLRR
                     TRDVGSGERAMQQRFFRMGEVILELVGPPSASGAGPCSIWGLALVTDDIDASAAHLGA
                     ACSAPKPAVQPGRRIATVRTRELGIGPTIALMTPHERATD"
     gene            5672..6430
                     /locus_tag="VLR27_07635"
     CDS             5672..6430
                     /locus_tag="VLR27_07635"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSP03354.1"
                     /translation="MTTVQTPISPATVSALLRLTADPDLEEADLAAVVAADAGLAARV
                     LAVANSAAFGLSRQVTQVHQAVGLVGMNLVQTLAIAGSCQLLDGSVGLPHARRHAVEV
                     ACAARALAERAGLNGGEAFSAGLLHDLGEILLWRQDPGPYAAAHAGWTDSATQLREER
                     GTYGTDHALVAREQLAGWNVPGNIVDAVGDHHRPDLLHRDLCTAVVAAEELTDRQIEW
                     SPRFEIFGVSPTAAPSLRDAVCEQADELLSLLVR"
     gene            complement(6431..>6729)
                     /locus_tag="VLR27_07640"
     CDS             complement(6431..>6729)
                     /locus_tag="VLR27_07640"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HSP03355.1"
                     /translation="TDDAAEITAQVIADGEHHTVVGRGNGPVSAFVSGLREHLDVDVD
                     VLAYTEHAIGAGADTQSVAYVETQTPEGLVRWGVGVHDSIVVASLRAVLSAVNR"
BASE COUNT          896 a         2434 c         2378 g         1021 t
ORIGIN      
        1 cggatcaggt agcggtcgtg ggtgacgagc aggaccgtgc ccgggtacac cttgagggcg
       61 tcctcgagca tgtcgcagct ggccaggtcg aggtggttgg tgggctcgtc gaggatcagc
      121 aggccggccg gctcggccat cgccttggcc agcgcgaggc gggtgcgctc gccgccggag
      181 agcatgccga cctggcgctc gaccgcgtcg tcgcggaagc cgaaggagcc caggtaggtg
      241 cgcagcgact tcccgcgggg cgtgtcgctg ccgatgccat cgcgggccac ctgcaggacg
      301 gtctggttgg gggcgagctc gtcggcctgg tgctgctcga agcgggcgac ggtgaccgac
      361 tggccgacct cggccgtgcc ggccatgggc ggcaggtcgc cgagcagcat cttcagcagc
      421 gtggtcttgc ccgccccgtt cgggccgacg agggcgatct tctggcctcg ctcgacgtgc
      481 accgacacgt cgcgcaggat cggcgtgtcg ccgtagccgg cggtcatgtg cgacagctcg
      541 gccaccaccc gtgaggtgcg gggcggctcc gggaactgga agcgggcctg cagctccttg
      601 cgatcgggga tctcgatctt ctcgagcttc tccagcatct tgatccggct ctgcacctgg
      661 cgggccttgg tggccttgta ccggaaccgc tcgacgaagc gctcggtctg ggcccgggcc
      721 ttggcctggc tcgccgcctg ggcctcgagc tgggcgatgc gctcctcgcg ctgcacgacg
      781 aactcggcga agccgccgac gtactcggtg agggtctggt tggccagctc cacgacgtgg
      841 gtcgccacgt tgtcgatgaa gtcgcggtcg tgggagacga acagcagcgc gccggtccac
      901 gcccgcagcc gggcctcgag ccagccgaca gagtccacgt cgaggtggtt ggtgggctcg
      961 tcgagcacca gcaggtcggg gctggagagc agcagtcgtg cgagggcgac gcgcatgcgc
     1021 cagccgcccg agagctcgga gacctggcgg tcggcgtcgt cgggtgcgaa gccgagcccg
     1081 gccagcacgg agtgggcctc ggcctcgagc gcgtagccgt tgagctgctc gtactgggtc
     1141 tgggcctcgc cgaaggcgtg cagggcctcg ggcgacgcgt cggccgggcc ctccacgatc
     1201 tggtggtgca ggcgcatgat gtcgggccgg ccaccgagca cggtgtcgag gacggtgccg
     1261 tcgtcctcgg aggggatctc ctgggccagg tagccgatcc gcatgtcctt gggcttggtg
     1321 accgaaccgg tgtcgggctc ggcgtcgccc accaggatgc tgagcagcga ggtcttcccc
     1381 tggccgttgc cgcccaccat cgcgacgcga cgtcccggtg acagcgacag cgtcacgtcc
     1441 tcgaacagga cgcggctgcc gtgcgcgagg gagagtccgg aggcggtgag catggccgtt
     1501 cgagcctacg gaggcggttc gcgaagccgc gaaacagatc gtctgcgtgg tcacgcccga
     1561 ccggtaccgt cgctccgatg gctgacggac ccctcgaacc cgacgcgctc gtgcgggccc
     1621 gcggcctgac gaagcgcttc ggggacttcg tggccgtcga cggcatcgac ttcgaggtcc
     1681 gccgcgggga ggtgttcggc ttcctcggtc cgaacggcgc cggcaagacg tcgacgatgc
     1741 gcatgatcag ctgcgtctcg ccgtcgaccg ggggcgagct gtcggtgctg cacctcgacc
     1801 cgagcgccga cgggcccgag atccgcggcc gcatcggcgt cgtgccccag gaggacaacc
     1861 tcgacaccga gctgtcggtc tgggacaact tcatcgtcta cggccgctac ttcggcctgc
     1921 cccggcgcgt gctgcgggag cgggcccagg agctgctcga gttcgcgcgg ctcaccgacc
     1981 gccgcgacga ccgggtcgac ccgctgtcgg gcgggatgaa gcgccggctc accatcgctc
     2041 ggtcgctcat caacgacccc gacctcgtgc tgctcgacga gcccaccacc gggctcgacc
     2101 cccaggcccg acacctgctg tgggaccggc tctacgggct caagcggcgc ggagtcacgc
     2161 tcatcatcac cacccactac atggacgagg ccgagcagct ctgcgatcgc ctcgtcgtca
     2221 tggaccaggg ccgcatcgtc gacgagggct cgccccgcga gctcatccat cgccacgtca
     2281 cgcccgaggt catcgagctg cgcttcgccg agcccgccga gcaggaggcc gcgctgtcgt
     2341 ccgccggcga ggccgccgag cgcatcgagg tgctcgccga ccgcttgctc ctctacacgc
     2401 gcgatgccga ggccacccac cgggccctcg ccgagcgcag cgtcgagccg ctgaccgagc
     2461 tgacccgccg ggcctccctc gaggacgtct tcctcgcact caccggccgg acgctggagg
     2521 agtgagccgg tgggaatgac gcagtcggtg tggcgggccc tggagcgaga gctgctcgtg
     2581 ttcgcccgca cctggcgggg caacgccttc accgccatcg tgcagccggt gctgttcctg
     2641 ctggccatgg gcatcgggct gggtggcctg gtcgacgagg gcgccggcac cgccagcgtg
     2701 cagctcgact acctcgagtt cgtcgccccc ggcctgctcg ccgcgtccgc gctgctcgcg
     2761 gtggccggcg aggcgctctg gggcgtgatg ggcggcgtca agtggatggg ccagttcaag
     2821 gggatggtgg ccacgccgat gacgccgggc gacgtgtacg gcggcctcgt cctgtccgcc
     2881 ggcgtgcgcg ccggtgccgc cgccgtcgcg ttcctcgtgg tggctgcggt gctcggcggg
     2941 gtgccctcgc tctgggcgcc gctcgccatc gtcgccgccg tgctgctcgc gatcgtcacg
     3001 atgagcgcgc tgtccgccta cgccgtgcgc aaggaggacg accggacctt cccgctcatc
     3061 atgcgcctcg gcgtcatccc gatgttcctc ttctctggca ccttcttccc ggtcgagcag
     3121 ctcccggatg tggtgcagcc gatcgcggtg ctctcgccgc tgtggcacgg cgtggaggtc
     3181 gcccgcgacg ccaccacggg cgccgtggac gggtggaccc tgctgcacgt gggcgtcctg
     3241 gtcgccctcg ctgcggcggc cctcccgttc ggtgtccgtg gcttcgagcg gcggttgacg
     3301 ccgtgagcgt ccaggacctg gtcgaccacg gccgcatcgc cccgccgatc gacgcccgca
     3361 agcgcttcct gcgcctgacc gagcgcaacg tcgtcgggta catgcgggcc tggccgatcc
     3421 tgctcaccgg cctggtcgag cccttcctct acctgctctc catcggcatc ggcgtcggtg
     3481 ccctggtcgg cgacatcacc tacgcgggac gtcaggtccc ctacgagacg ttcgtggcga
     3541 gcgggatgct ggccacggcg gcgatgaacg gcgccgtgtt cgacaccacg ttcaacttct
     3601 tcatccgcct gcgctacgcc aagctctacg acgcggtgct ggcgacgccg ctcgaacccc
     3661 gagacgtcgc gctgggtgag gtcacctggg cgttgatgcg gggcaccttc tactcggcgg
     3721 cgttcctcgt cacgatggcg ctgttcgggc tcaccgagtc gtggtgggcc atcgcggcgc
     3781 tcccggccac cgtcctcatc ggtgcggcct tcgcctccgt cgggctcgcc ggcgccacct
     3841 tcctgcggtc gttcctcgac ttcgacttcg tgaacctggc catcacgccg atgttcctgt
     3901 tctcggcgac cttcttcccg ctgtcgcagt accccgacgc gctcgccgcc gtcatccggt
     3961 acacccccct ctaccagggg gtggcgctgt gccgggggct caccaccggt gaggtcggct
     4021 tcggcctgct cgtgcacgtc gcctacctgc tcgggatggt cgtggtgggc gtggccgtgg
     4081 ccagccgccg gatcgacggc ttgctccgct cctgacgtcg accgactccc tcccggtcgt
     4141 tccgggtacg gagcacggga gcccagaccc tgcccccgat gcaaggagcc gcaccatgat
     4201 ccacgagatc gacatcgacc tccccgtgaa caccgtctac aaccagtgga cgcagttcga
     4261 ggacttcccc ctcttcatga agcacgtcgt ggacgtccgc cagatcgatg acacgcacac
     4321 ggcgtggaag gccaagatct ccggcatcag ccgcgagtgg caggccgaga tcaccgagca
     4381 gaccccggac cagcgggtcg cctggaccgc caccgatggc accgagaact ccggcgtcgt
     4441 gacgttccac gccctcgacg acaacaccac gcgcgtcgtg ctgcagatgg agaccgatcc
     4501 caagggcttc ctggagaagg tcgccgactg gggtggctac atctcggacc gctccaagaa
     4561 ggacctcgag cagttcaagg acttcatcga ggcccgcggc aagtccaccg gcgcctggcg
     4621 cggccaggtc gagcgcgact ccatgcgcga cctgcactcc cgcgaggacg agctccgcca
     4681 gctcagcgac cacgagctgg ccaagcgagc agaggacgcc gggctcgagc ggccgctgga
     4741 ccgcaaccgc gactggctcg tcagcgccct cgctcgtgac ggctacgagc gcaggaccgg
     4801 ccgcgaccgc ggtggcgagg gcgaccacga cgaccgccgg gaccccgagg acgcgcacac
     4861 gtcctgaggc acctgcgagc gggggcggtc aatccgtcgc ccgctcgtgc ggggtcatca
     4921 gcgcgatggt cggaccgatg ccgagctcgc gggtgcgcac ggtggcgatc cgtcgccccg
     4981 gctgcaccgc gggcttcggc gcggagcagg ccgcgccgag gtgggcggcc gacgcgtcga
     5041 tgtcgtcggt gacgagcgcc aggccccaga tgctgcaagg cccggcgccg ctggccgacg
     5101 gcggccccac cagctccagg atcacctcgc ccatccggaa gaagcgctgc tgcatcgccc
     5161 gctcgccgct ccccacgtca cgggtgcgcc gcaggtcgag gcccagcgcg ccgaacgcct
     5221 ccgtcgtgcg gtcgaggtcg ggggagcgga gcacgatgtg gtcgatgcgc accacgccgt
     5281 tggggtgcgc caccggtcgt ggcggctccc ggtcggtggt cgacgtcgcg atgccgtcca
     5341 ccgagcgcgg cacgtcccca tcggcgcgga gccgccagcg gtcggcgccg gcaccatcgc
     5401 cggtgcagct gatccgcacc gtgccgacgg cgacctccgc gccctcgacg gtgaagcccg
     5461 ccgccatcca cgccgccggt tcgtcgccca cctccagagc ctccaaccac accgtcacgg
     5521 tgccggtcta ctgcatctcc caggacgccc gaggtgcctc cggaccagga actgggagat
     5581 cgccctcaac tcccggctga tccggtcgaa ggggagatca cgtcgagcca gcggcgcgag
     5641 gagcacgtac gagcatggtg atcgagggat catgaccaca gtccagacac cgatctcacc
     5701 cgcgacggtg tcggcgctgc tgcgcctcac cgccgacccg gatctcgagg aggccgatct
     5761 ggctgcggtc gtcgccgcgg acgccggtct cgcggcacgc gtcctggccg tggcgaactc
     5821 cgccgccttc gggctgtccc ggcaggtcac ccaggtccac caggccgtcg gtctggtggg
     5881 gatgaacctc gtgcagaccc tggcgatcgc cgggtcctgc cagctcctcg acggctccgt
     5941 cgggctgccc cacgcccgcc gtcacgcggt ggaggtggcc tgcgcggccc gggcgctggc
     6001 cgagcgcgcc ggcctcaacg gcggtgaggc gttctccgcc gggctgctgc acgatctcgg
     6061 ggagatcctg ctctggcggc aggaccccgg gccctacgcc gctgcgcacg ccggctggac
     6121 cgactcggcg acccagctcc gcgaggagcg cggcacctac ggcaccgatc acgcgctggt
     6181 cgcgcgtgag cagctggcgg ggtggaacgt gccgggcaac atcgtcgatg ccgtgggcga
     6241 tcaccaccgc ccggatctcc tgcaccggga cctgtgcacc gccgtcgtcg ccgccgagga
     6301 gctcaccgat cgccagatcg agtggtcgcc gcggttcgag atcttcgggg tctccccgac
     6361 ggctgctccg tccttgcgcg atgcggtgtg cgagcaggcc gacgagctgc tcagcctgct
     6421 cgtgcgctga tcagcggttg acggcggaga gcaccgcccg cagggaggcg acgacgatgc
     6481 tgtcgtgcac cccgacgccc cagcgcacca ggccctcggg cgtctgggtc tccacgtagg
     6541 ccacggactg cgtgtcggcg ccggcgccga tcgcatgctc ggtgtaggcg aggacgtcga
     6601 cgtcgacgtc gaggtgctcg cgcaggccgc tcacgaaggc cgacaccgga ccgttgcccc
     6661 gcccgacgac ggtgtggtgc tcgccgtccg cgatgacctg ggcggtgatc tcggcggcgt
     6721 cgtcggtgg
//
LOCUS       DATISU010000188        26926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_381768, whole genome shotgun sequence.
ACCESSION   DATISU010000188 DATISU010000000
VERSION     DATISU010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 26926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26926
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381768"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VLR27_07645"
     CDS             complement(<1..887)
                     /locus_tag="VLR27_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03356.1"
                     /translation="MPDAPDRPPAAWVFEVDPTDIDLGSAFADGEAMIQFEVADDRAA
                     LLGPGQLAHLWVQDRLGEVVPPGIYATGSVVGPVELLDDEDGGRVAVVSVGFDSLRSP
                     IDGTSLLAGDEFVGSPLHDGAAEQNPVPLDAAQAAAVAANDLDPGPVATVLLGVEADG
                     EGITLPALEVRLPSDTLLVVEGVDHDGWTVVRGTPGMDEVTELPEQHATFIDAVEYVA
                     AAVGKAGGSLPVVDTDEGIEAVAIFDADGGVYAVLKEAEDAYTFAWIDDDGDLERLDT
                     YATLKEAILLPVLDEELFGG"
     gene            954..1637
                     /locus_tag="VLR27_07650"
     CDS             954..1637
                     /locus_tag="VLR27_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSP03357.1"
                     /translation="MAADEPSTPEAEDLVRFMRMVSHGLRNPLAIATGMLDMLEQLAG
                     AQLDEDSRELLTRSSAAVHRTADMVLSVQRYIGAQHRPLQTGPVDLGEVARWVAEQLD
                     PAEVVVHVADGLPTLTADQDMVEWVFHELFDNARRHAGDGGVVTVNVELDREDDRWLV
                     SVTDDGAGIPAERHEDAFGEGDRLDRSGGGLGLGLPTVRAVLSRHGGKAWLEDAPDGG
                     LRAVTAWPA"
     gene            complement(1711..3783)
                     /locus_tag="VLR27_07655"
     CDS             complement(1711..3783)
                     /locus_tag="VLR27_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSP03358.1"
                     /translation="MSDIRGFAVEEVIEDFRLACVSRAIDDREISLQKQSKVFFQISG
                     AGHEALLLGLARSLRPGHDWFFPYYRDLALMLGLGISPYELLLQAVGSAECRASGGRQ
                     MPSHWGYAAEHVVSQSSPTGSQCIPAVGCAEAGRYIGRTPELGHLPAAGDEVTYVSLG
                     EGATSEGEFWESLNTACTLHLPVVYVVADNGYAISVPVSDQSPAPVSELVRGFRGLEV
                     LRLDGTDYLEVRGWCDAAIARVRAGVGPLLIHADVTRPYSHSAADTQAKYRSERELAD
                     EAERDPIGRLEQVLVEHGALSPSEASAIRAEARQTVADAAKRALGAPRPDPATITDHV
                     VALPDHPVPPDDYEGGAEVALGGAISAVLHERMAADVRIRVFGEDVADAREQVLANVE
                     GKGGVFGTTHGLQRDFGMARCFNTPLAEANIIGRAVGHGVRGLRPAPEIQFFDYVWPA
                     MQQIKSEAATMRWRSNGAFTAPMVVRIPIGGYLHGGAIWHSQSGESIFAHVPGLLIAF
                     PSRARDAAGLLRYAFECEDPVLFLEHKHLLRQPYTKDLLPPADWVLPFGVADVRRPGD
                     AVTVITWGAMVQKSLVAAEAIAEETGRQVEVIDLRTIIPWDKDRVGESVQRTGRALVV
                     HEDVVTAGFGGEIAAWIADECFWSLDAPVRRVGAPDVHVAYEPTLEDAILPQADDVTA
                     ALRSILAE"
     gene            complement(3876..4607)
                     /locus_tag="VLR27_07660"
     CDS             complement(3876..4607)
                     /locus_tag="VLR27_07660"
                     /inference="COORDINATES: protein motif:HMM:NF022432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HSP03359.1"
                     /translation="MAHLDPDGAGGGAPDPGVLHKVRSLLAKAESTSYPDEAEALSAK
                     AQQLMERHAIDRAMLGEASRSRPEGRTLLVESPYARPKFQLLTELAKANRCRAVLDTT
                     DNVATVVGFPDDQATVELLYTSLLVQGTRAMLQAGTSQRTRSRAFRQAFLVAYASRIG
                     QRLRQASAATVSEAVREHGEGLLPVLRSREVAVEDELASAFPHLRRMRVTASDPAGYA
                     AGHAAADRARLNGQEVRGRMRRLRR"
     gene            4709..6019
                     /locus_tag="VLR27_07665"
     CDS             4709..6019
                     /locus_tag="VLR27_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HSP03360.1"
                     /translation="MRRLSPTDAVDLFRTTDTLGLPLGPSQPAALLEAMGSRKDWEDL
                     KVFGALVTVLTDLFTHHNVHYLSGFFGPLERLLRDSGANIGFAPADFRRFVTLLESIS
                     PRVMATAAAPPDRDGWCSLSLHAGANTGSLHAAGADPDRLLVVEVSDGYPVTYGWGEH
                     GHRLHVDEIDVLIESESTPYEIADPEPTDVERAIARHIQGLIPDGATLQTGIGAVPSV
                     VAALLAEGDGGDYGIHSEMFTTGLMKLHQAGKVTNARKGQYEGVSVCTFAGGTRELYD
                     WLHENRDVIFLPVEVVNSPELIGANQTMITINAALSVDIHGQVVADTRSGEQFSGIGG
                     HEDFVAGTGLDLTDRSILCLPSTYRAGEEVRSRIVPWFAAGTVITTPRHQVDVIVTEH
                     GIAELQGKTVHQRGEALAAIAHPDFRDGLLEAAQRASGGASPMP"
     gene            complement(6191..7120)
                     /locus_tag="VLR27_07670"
     CDS             complement(6191..7120)
                     /locus_tag="VLR27_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HSP03361.1"
                     /translation="MHLITGLGQALEDAADQNHLLATSDLTRLGLDRKQVARLCDDRI
                     IERIVRGLYRVAGTRCPQQDIAAAVCRHAGAVASHESALWLHDIDVDPPEPPHLTLPP
                     GATSHTTLGVLHRSPLERIDVTRRRGIPVTTLARAVVDASAHLSVTALHEVTAQAVTA
                     RRTTVRHIEEAARRVEAEPGRIGHGTLRTVLESWAGRILPDSVAEATVLRRIIGFGLA
                     RPVTQHEVRDDAGRFVARVDLAWPDERVCREYDSDGYHPPPKVEEDERRRARIEAAGW
                     SVGVIHRGHLLPSREDWLRRLAEDLRRRHRRAG"
     gene            7302..8903
                     /locus_tag="VLR27_07675"
     CDS             7302..8903
                     /locus_tag="VLR27_07675"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HSP03362.1"
                     /translation="MGLSRRTFIKTAAVAGAGAALGGPAHADLLNLTRPTSLLLDGPA
                     PKFPNIQHAVVVMMENRSADHYLGWWGDREDVRFDASTTLPGRETFDFGIDGEQNWAG
                     HPYLDPGHSHTTGRRQILDHDGDGTPDGWSTTEDGSDRPGTGTDRYASSYYTAADLPV
                     IDRIVQDFTSFDRYFTSWMGNTYPNRYYMHSAQAHGLNNNDFPPQRIGENPAWAAGWD
                     WPTLWDQLNSRGVTWKYYFSNLPVIALWGPRYLANTRPITEYYADCAAGTLPQVAFVD
                     PFFVAPEGLANDDHPHADIRLGQEFFSDVTTAFMRSPNWSKGAMFINYDEWGGFFDHV
                     VPPAVDNTVDPYGAYAPGEVPDYTVEGPHTAGPDDFGLFGFRTPAFLLSPYARNTGVN
                     HRAPAGSMPPKSKGKGQGRRSDSEPTVDRTVSAPLDHVSILSFICENWGLPPTEQWDP
                     GRSRHAIGTMRDAFDPSIADPDHPGDPEVDVDAYEYVAPPEARTTALPIPASSVSDLW
                     VMADWIEAQGYRINTRFTHALPFTR"
     gene            complement(8925..9296)
                     /locus_tag="VLR27_07680"
     CDS             complement(8925..9296)
                     /locus_tag="VLR27_07680"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HSP03363.1"
                     /translation="MSTLGTGPTIFHNPRCSKSRAAMSTADELGVDVDEVRYLDSPPD
                     RATLEAIVAGLEDPPTDLIRHGDAKKAGVDTSQATTPTAVVDLLVEHPELMERPVVWL
                     HGRAIIGRPPERVAPFLQDNA"
     gene            9341..9559
                     /locus_tag="VLR27_07685"
     CDS             9341..9559
                     /locus_tag="VLR27_07685"
                     /inference="COORDINATES: protein motif:HMM:NF037605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HSP03364.1"
                     /translation="MTSPRGIDEPHPSRLAPDDPHRDRILAAHRAAVDAGEPGYLDPA
                     TGLFVLTAVELAAAGSCCERGCRHCPYV"
     gene            complement(9457..10278)
                     /locus_tag="VLR27_07690"
     CDS             complement(9457..10278)
                     /locus_tag="VLR27_07690"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSP03365.1"
                     /translation="MNDLDDVAALGTVLGIWAHPDDETYLSAGVMAIAAGNGQRVVVV
                     SATAGERGTDDPVAWPPDRLAPVRRWEASAALAALGVHDHRWLGLPDGELARCSATEQ
                     AGRLAAIIDEVAPDTILTFGPDGVTGHPDHRAVSSWVEAACRCSDRRVRVLHAVLEEG
                     YCERFRGLHERFDVFMDPALPAPRPVADLALRLSLTGASLDRKLVALRAQATQTQPVV
                     EAFGPDPYAEWVAEEAFVDATAQTYGQWRHPRSQHEPAAASSTAVSTNSPVAGSR"
     gene            complement(10275..10961)
                     /locus_tag="VLR27_07695"
     CDS             complement(10275..10961)
                     /locus_tag="VLR27_07695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP03366.1"
                     /translation="MTTTDPRLAPDPAPTEHGTFHHGARSEFNAWFFRTFDRYLNHIT
                     RQHKRSAFADIAAGDIVELGAGVGANFAHLPPGSRVLAVEPSEAMHEQLEARAAARGL
                     DLIVIGAYGEDLPIPDASVDEVLCSLVLCTVDDPEAVLAEIRRILRPGGRFRFVEHVA
                     APSWSPRRWLQHAIRRPWSWVFEGCDLCRDTAATIEVAGFRQVAIRRARLKRSAFVPV
                     NTAISGCAVR"
     gene            11138..13291
                     /locus_tag="VLR27_07700"
     CDS             11138..13291
                     /locus_tag="VLR27_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSP03367.1"
                     /translation="MRPRQPTESGVVDRDGVGIHWETYGSGEETLVLLPPWMIVHSRI
                     WKAQVAGLADHFRIVTYDARGCGRSDRPTDPAAYGPEAHTGDALAVLDATGTERAVLV
                     GNSYGGLLALVLAAFAPERVAGAVFIGPSTDLSGTADAPLTRALLHFHDEPDVEEGWG
                     RYNRHAWARDFPGFVRWFTETALAEPHTTKPREDALSWGLDTTAEVLTATVAARAGVP
                     PEKSAAALRALIPAVTCPTLVIQGLLDEICPPTFGRDLAGALGSELVEMPDAAHCPQV
                     RFPIQVNELIRSFVARRVSTDTTGPVGVVCDPGHAPAPRRTRPPRVLYLSSPIGLGHA
                     RRDLAIASELRTLVPEAEIDWLAQDPVTRVLDAAGERVHPASRRLASESAHLEAETDE
                     HDLHVFEALRRMDEILVTNFSVFSDVTASGEYDLVVGDESWEVDHFLHEHPATKRARF
                     AWLSDFVGIVPMPSGGEREAFVAADWNREMIGHVERQPGIRDRSIFVGDPEDVVPLGF
                     GPGLPSIAEWTAAHYDFSGYITGFDPAELQHDVLRAELGYRDDEVVVIAAVGGSGVGL
                     PLLRRIVAAHPLAAQEIPGLRTVVVTGPRLDPNALPDVEGVEKRAFVPDLHRHLAACD
                     LGIVQGGLTTTMELTAAQRPFLYVPLQNHFEQQVHVRHRLDRHGAGRALDYAAATPEV
                     IAEAMTEQLRQGPPRYQPVRPDGARGAASLLVDLL"
     gene            13350..14540
                     /locus_tag="VLR27_07705"
     CDS             13350..14540
                     /locus_tag="VLR27_07705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP03368.1"
                     /translation="MTITEHPTTTGRQLPDDDVLAGFRARARTSDDRNEYFHEDLAIL
                     REIGYLGAAVPTELGGWGHDLRQLARSQRRLASFAPATALAMTMHHYWVGIAMEHERV
                     GDTSLRWVLERAAAGEVFAAGHAEVGNDAPVVMSTATAERVDGGYRFNGHKMFGSNGP
                     VWSWLGVHALDASDPERPVVVHGFVPRDADGVTVVPNWDTLGMRPSQSYDTILDDVFV
                     PDDRIGAITPAGDESDLFLFAMNLWFVGQCGSVYLGIADRALELAVRSATSKTSVAIP
                     RGTYAHNPMVQHQVAEMYLELDAADATVRAFLDDVVDGIDHGDLWGAKIVSAKWRAVE
                     AAKRVVDRALDVAGGGGMVRGNELERLYRDVRCGGFHPTNAALTFETAGKTVLGIDPS
                     GPRW"
     gene            complement(14541..17438)
                     /locus_tag="VLR27_07710"
     CDS             complement(14541..17438)
                     /locus_tag="VLR27_07710"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HSP03369.1"
                     /translation="MNAIAVEVLGPLRVRDAAGGDVTPRGRLQRRLMAFLALRAGAVV
                     SIDQLTEVLWPVRLPTDAASLVHSHIFRLRQHIPDIELEATPPGYVLRLPEGGIDAAR
                     FERAVARAAAERVGDPEGALEALDRALRSWRGEAFEDLVDLDAGRIEAERLEELRLRA
                     VEERFAALLDLGRGADAVADLEALAAREPLRERPRELLMDALVASGRRADALRVYDAY
                     RRQLAEELGVEPSAHLRSVHERLLDGDPVPQRRPAAGATAVARPLPRPVSSFVGRAQL
                     LGVLAELVQHERLITLVGPGGIGKTRLVTELCHLVVESFPDGARFCDLTAVDDDGVPA
                     AVAATVGVEDRPGVSTTERVAEVIRHERSLLVLDNCEHVLDGVASVADELLRSTERVV
                     LLATSRERIAVDGERLFPVDPLGASGEESPAVLLFADRAVAASPSFALDDRTRPLVVD
                     LCQRLDGLPLAIELAASRLASLSLEEVIAGLAHSTAVLRGGPRTVPRHRSLDAALAWS
                     HRMLAAEDQLVLAAAATFAGAFDAEDVAAVADRSAFDVHDRLADLVERSLVHRDDGRF
                     RLLQVVRSFLAERSEGTRRDELARRHADRVAARVSAASSALRTARDDAPMQAVRALVP
                     DLRQAMAAATARRDPDLALRIVVAVRDPAMHGMLPELMAWGEPAGDLGAAAGHPLAVD
                     AYAVAALGAWKSGDLERMGHLLDRAEAVAAELGLAPTYELIGARATESLAHGALADAV
                     EGFRAGLRLDDAVGDPLRQGEDGATLAIAESYAHAPEAVADAERLLDEVAPGAGAIAA
                     AWCWYGAGEVLLDDDPPEARRRLRSAVELARAGGGQLVEGVAGASLASLDARAGDVRA
                     AVGHYRWLLPLWLRAGVRSPLRTMLRTVTELLCGQGLDEQAALLLGAVTAPGVGHEVV
                     GDDVDRLAAVRAELEQRLGSARLDELLVEGATLDDAAAASVATAAFELIEG"
     gene            complement(17502..18389)
                     /locus_tag="VLR27_07715"
     CDS             complement(17502..18389)
                     /locus_tag="VLR27_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP03370.1"
                     /translation="MLTPPAPEEVAAVVDLIVDQQRRPDRNIPYLGTDPEGVTAELEG
                     LEPAWTSTARIAREDDRIVGAVAVEWDDDVGRSWVMGPWVAHDDDRWLPLAGELLDAA
                     LAQVPSSITRHEVSGDLANQQVAALAAARGWWPTEVNHALSVDEAIVGTWGDIDVTGL
                     RPATTDDLEGIRPLHDAEFPGTYATADQLVAGQQDGSRVTLVADDGAGGVAAYASGHL
                     QDDGEGCLDFVVVHPDQHGRGLGRQAVAAIARDLLPRSPLGRMSLTVQDHRAPARGLY
                     ERLGFRHEASFVGFRSWEE"
     gene            complement(18399..19202)
                     /locus_tag="VLR27_07720"
     CDS             complement(18399..19202)
                     /locus_tag="VLR27_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP03371.1"
                     /translation="MADDLGAMRAYYAQDKERDRLAFGLGRVELARTIEVVGRTLPPP
                     PAVVADLGGGPGRYTDWLVEAGYDVVHRDVVEHHVAQVRARHGERVDSAVGDARALDL
                     PDASVDVVLLLGPLYHLVQAGDRGRALQEAARILRPGGILHAAAISRWATRMDGILTN
                     RFHLLFPGMVDAVDEAERTGHLPPIVEAGFTGYTHRPDELRDEVTAAGFEVQSLVSLE
                     GVAFALADLDERWDDDTERTLLLDTLRALESVPELLGVGPHLLATGRRI"
     gene            19270..19917
                     /locus_tag="VLR27_07725"
     CDS             19270..19917
                     /locus_tag="VLR27_07725"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP03372.1"
                     /translation="MPGTIDRLPDLEIPGHQPSRPDGADHPMRVMTRRAAGLQAPPWD
                     ARARHEVAALFDGLAPEWHTRTSPERARVVADALDRGEVGPGATALELGSGIGTYTAT
                     LGERFGEVVAMDLSLEMLRLAADGPGHRVLADAHRLPLTDASVDAVVLVNMLLFPEEV
                     DRVLRPGGCVVWVNSSGEATPIHLLPEEVVDALPGAWNGTWARAGTGLWTVLRRD"
     gene            complement(19914..20906)
                     /locus_tag="VLR27_07730"
     CDS             complement(19914..20906)
                     /locus_tag="VLR27_07730"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HSP03373.1"
                     /translation="MPPVARTAIPRSLVEKLERALRTGEDALSALADAQPVDERDRLL
                     TLQVIHDLHVGDIDRVGDAVRWQHHPAVAALKTHLEQDVLAELDAMIEGDATDGDDDA
                     VATMRVIARQDLVPRIYEWVAADATWDGLVDFLALEGGPDAGFDDLVAACQIGLRGEP
                     KLELARNYWDEMGRGELDEIHTELHHDLCEAIDMPHVPRSELPTSALLRSSLGGILAT
                     NRVLQPEMVGALGLLELQAGPRCRQVVKAFDRLDAPAGAYPFYVEHRDVDPHHGKAWL
                     DNAIAPLAGDPRWAAGMVRGARWRSAANAVFFADMAERFADVGSDAPLVSAVAG"
     gene            complement(20997..22187)
                     /locus_tag="VLR27_07735"
     CDS             complement(20997..22187)
                     /locus_tag="VLR27_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HSP03374.1"
                     /translation="MTADLLDAARALQPDTVALRRDLHRHPEIGNDLPRTQGAVLEAI
                     GDLGFDVRTGTGVTSVTATLDTGRPGGTMLLRGDMDALPMPEDTDLDYASEVDGAMHA
                     CGHDAHTAMLASAARLLADRADDLTGKVVFMFQPGEEGHHGARHMLEEGLLDGLHVTR
                     AFAIHQSPSFPSGMVATKAGPLLASADEFVVEVVGRGGHAAMPHHANDPVPVACAMVG
                     AFQTLVTRRVDVFDPAVVTVGSIRAGSTNNVIPETARLVGTIRAVSERTRALIHAELE
                     RIATNIAAAHDMEVRFRIDDGFPVTVNDADAAAWVGDVARRIVSPDLVVDMPNPVMGA
                     EDWSYVLQQVPGAMAFLGTCPPGVSFHQAAPNHSNRMVIDEDAMAVGVALYAATALDE
                     LAPA"
     gene            22263..22475
                     /locus_tag="VLR27_07740"
     CDS             22263..22475
                     /locus_tag="VLR27_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03375.1"
                     /translation="MTADSDDSIPEQVEDGELEDPMDDEDEDEHESVIDRMRGSGVGV
                     EDPDIVGDAHPGTVDAEIAEDEPPQV"
     gene            complement(22489..22986)
                     /gene="tpx"
                     /locus_tag="VLR27_07745"
     CDS             complement(22489..22986)
                     /gene="tpx"
                     /locus_tag="VLR27_07745"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HSP03376.1"
                     /translation="MAEITLGGNAVHTNGELPEEGAKAPAFELIKSDLTPVSTDDLAG
                     QRVVLNIFPSIDTAVCATSVRTFNERAADLDDTTVVCVSADLPFAAARFCGAEGIENV
                     VTGSTFRSSFLDDYGVRMTDGKLAGVAARAVVVLDGDGTVLHRELVPEIAQEPDYDAA
                     IKVLQ"
     gene            23073..24548
                     /locus_tag="VLR27_07750"
     CDS             23073..24548
                     /locus_tag="VLR27_07750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP03377.1"
                     /translation="MTPPAQHRERIQARLTDSDRYPRWVLLTALAGMFATTFPVTLLA
                     VSIPTIADDFGAPQTLVAWVISGPLLASAVALPILGKLGDLYGQRRVFLIGFVVSTVV
                     TALTTLAWDPYSLIVLRGLAVVVGAATIPSSMALINSVHAPAQRAKAMGWWSLVAAGS
                     PAIGLIIGGPLIDAIGWRPLFGVQAVLSVIPVVVAWLVLRETTPRRDVGFDVPGAGAL
                     ALGAGGIMLALNQLPSRGFDPLVAGAAAVGIAGLAAFVAVERRVAYPLLPLELFRRRN
                     FTASMLGSLFSGAAYMGGFVLAPLLLQSVFGYSVAASSFIMLLRPASFSATSPVGGWL
                     ATRIGERPVAVFGATAIAAALLALGFGASEELVVLVMGALVLQGIGNGTAQPTLTATL
                     SNSVEEHDLGVAAAAQRMMFQVGAALGITVMISVYGGTGEASDFFNGYAVGSVLGIAA
                     IVFSWFIRSTPRGPAADDEVSEETAVGATPPAAPVSVTAGD"
     gene            complement(24588..25082)
                     /locus_tag="VLR27_07755"
     CDS             complement(24588..25082)
                     /locus_tag="VLR27_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HSP03378.1"
                     /translation="MTLTTRAPRHAHTAPPDRRRWSTKRIALVLAAPLLVLGLAACDP
                     TPDQESVRSHVNESRRAHGVHALGDDLAVRLKAQDWANRLAEAGNLSHSTLSSGLDGV
                     PWVAVAENVGRGSSIGSVHDGYMRSPSHRANILNPRWDRIGTGHAVARDGTVYTVHVF
                     VDLG"
     gene            complement(25223..26782)
                     /locus_tag="VLR27_07760"
     CDS             complement(25223..26782)
                     /locus_tag="VLR27_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HSP03379.1"
                     /translation="MAPPQPTETTDDLPWWKTAVVYQVYPRSFADADGDGVGDLPGIL
                     ANVDHLADLGVDCVWMSPFYESPMRDNGYDVSDHCAVDPVFGSMEDFDALLAALHDRG
                     IRLIVDFVPNHVSSDHPWFVEAVSHPESDTRDWFIWADPAPDGGPPFNWTASFTKESA
                     WALDEQSGQYYLHLFLPDQPDVNWSNPDLEAAQHDVLRFWLGRGVDGFRVDVINAIGK
                     DPDPKDVPERMVGIDHVVLTHHETAMPLIRRIREVVDPPGVERLVVGEVVTGDLEQQA
                     SYCGDGDGVSCTVNFVSVGAGWDRDAWHLLIAGMEDAYADHEATWPSYVLSNHDNERH
                     RTRYGGSEARARSAAVLLLTVRGTPFLYQGEELGLEDAVIPAGTEVDPGGRDGCRAPI
                     PWTVEEPHSWGEDPWLPFPPEAATRSAEAQRADSHSVLALYRQLLAARKASPALQQGS
                     LELFARTDDVLAYRRVHGDDERIVAVNFGDAPADVTDLTGTVVVGSNGLGAGHPFSGR
                     LGPDEAVVLQP"
BASE COUNT         3672 a         9774 c         9879 g         3601 t
ORIGIN      
        1 ccgccgaaga gctcctcgtc caggacgggg agcaggatcg cctccttgag ggtggcgtag
       61 gtgtcgaggc gctcgaggtc accgtcgtcg tcgatccagg cgaaggtgta ggcgtcctcg
      121 gcctccttca gcacggcgta gaccccaccg tcggcgtcga agatcgcgac ggcctcgatg
      181 ccctcgtcgg tgtcgaccac cggcagcgaa ccgccggcct tgcccaccgc ggcggccacg
      241 tactcgacgg cgtcgatgaa ggtggcgtgc tgctcgggga gctcggtgac ctcgtccatg
      301 ccgggcgtgc cccggaccac ggtccagccg tcgtggtcga cgccttcgac cacgagcagc
      361 gtgtcgctcg gcaggcggac ctcgagggcc gggagggtga tcccctcgcc gtcggcctcg
      421 acgccgagga gcacggtggc caccggaccg gggtcgaggt cgttggcagc cacggccgcg
      481 gcctgggcgg catcgagggg caccgggttc tgctcggctg cgccgtcgtg gaggggtgag
      541 ccgacgaact cgtcgccggc caggagggac gtgccatcga tcggcgagcg gaggctgtcg
      601 aagcccacgg acaccacggc cacgcgcccg ccgtcctcgt cgtcgaggag ctcgaccggg
      661 ccgacgacgc tgccggtggc gtagatgccg gggggcacga cctcgccgag gcggtcctgg
      721 acccacaggt gggcgagctg gccgggaccg aggagcgcgg cccgatcgtc ggccacctcg
      781 aactggatca tggcctcgcc gtcggcgaac gccgatccga ggtcgatgtc ggtcgggtcg
      841 acctcgaaga cccaggcggc gggtgggcgg tcgggagcgt cgggcacggg tcgaccctac
      901 gggcgcgtgc ctccgacggt tgccttgccg agacggtgtg gtcccatggg gcggtggctg
      961 ccgacgaacc ctccacgccc gaggccgagg acctcgtccg cttcatgcgc atggtgtccc
     1021 acggcctgcg caacccgctc gccatcgcca ccgggatgct cgacatgctg gagcagctgg
     1081 ccggcgcgca gctcgacgag gactcccggg agctgctgac ccggtcgtcg gccgccgtcc
     1141 accggacggc ggacatggtg ctgagcgtcc agcgctacat cggggcccag caccgaccgc
     1201 tgcagaccgg gccggtggac ctgggcgagg tcgcgcgctg ggtcgccgag cagctggacc
     1261 ccgccgaggt cgtcgtgcac gtggccgacg ggctccccac cctgaccgcc gaccaggaca
     1321 tggtcgagtg ggtcttccac gagctgttcg acaacgcccg gcgccacgcc ggcgacggcg
     1381 gggtcgtcac cgtcaacgtg gagctcgacc gtgaggacga ccgctggctg gtgtcggtga
     1441 ccgacgacgg cgccggcatc ccagccgagc gccacgagga cgccttcggc gagggcgacc
     1501 gcctcgaccg ctccggcggc gggctcggcc tcggactccc gacggtgcgg gcggtgctga
     1561 gccgccacgg tggcaaggcg tggctggagg acgcacccga cggcggcctg cgcgccgtca
     1621 ccgcctggcc ggcctgacgt cgcgctacac cacgactgcc agtttccgag gactggaact
     1681 ggcagttctc ctgtcgcggc tggggaggcg ctactcggcg aggatgctgc gcagggccgc
     1741 ggtgacgtcg tcggcctggg ggaggatggc gtcctcgagg gtgggctcgt aggcgacgtg
     1801 gacgtcgggg gcgccgaccc ggcggacggg ggcgtcgagg gaccagaagc actcgtcggc
     1861 gatccacgcc gcgatctccc cgccgaagcc ggccgtgacc acgtcctcgt gcacgacgag
     1921 ggctcgcccg gtgcgctgga ccgactcgcc gacccggtcc ttgtcccacg ggatgatcgt
     1981 gcgcaggtcg atcacctcga cctggcggcc ggtctcctcg gcgatggcct cggcggcgac
     2041 cagcgacttc tggaccatgg cgccccaggt gatgacggtg actgcgtcgc cgggacgccg
     2101 cacgtcggcg acgccgaagg gcagcaccca gtcggccgga ggcaggaggt ccttggtgta
     2161 gggctggcgc agcaggtgct tgtgctcgag gaagagcacc gggtcctcgc actcgaacgc
     2221 gtaccggagc aggccggcgg cgtcgcgcgc ccgggagggg aaggcgatga gcaggccggg
     2281 gacgtgggcg aagatcgact cgccggactg gctgtgccag atggcgccgc cgtggaggta
     2341 gccgccgatc gggatgcgca cgaccatcgg cgcggtgaag gcaccgttgg agcgccaccg
     2401 catcgtggcc gcctcgctct tgatctgctg catggccggc cacacgtagt cgaagaactg
     2461 gatctccggt gcgggccgca ggccccggac gccgtgaccg accgcccggc cgatgatgtt
     2521 ggcctcggcc aggggcgtgt tgaagcaacg ggccatgccg aagtcgcgct gcaggccgtg
     2581 ggtggtgccg aagaccccgc ccttgccctc gacgttggcc agcacctgct cccgggcgtc
     2641 ggcgacgtcc tcgccgaaga cccggatgcg cacgtcggcg gccatccgct cgtggagcac
     2701 ggcgctgatc gcaccgccga gggcgacctc ggcgccgccc tcgtagtcgt cgggcgggac
     2761 cgggtggtcc gggagggcga cgacgtggtc ggtgatcgtc gccgggtcgg gtcgcggggc
     2821 gccgagggcc cgcttggccg cgtcggcgac ggtctggcgg gcttcggccc ggatggcgct
     2881 ggcctccgac gggctgaggg cgccgtgctc gaccaggacc tgctcgaggc ggccgatggg
     2941 gtcgcgctcg gcctcgtcgg ccagctcgcg ctcggagcgg tacttggcct gggtgtcggc
     3001 ggcggagtgc gagtacggcc gggtgacgtc ggcgtggatc agcaacggac cgaccccggc
     3061 ccggacccgg gcgatggcgg cgtcgcacca gccccggacc tcgaggtagt cggtgccgtc
     3121 gaggcggagc acctcgagcc cgcggaaccc acggaccagc tccgagaccg gggccggcga
     3181 ctggtccgac acgggcaccg agatggcgta gccgttgtcg gccacgacgt agacgaccgg
     3241 gaggtgcagc gtgcaggccg tgttgagcga ctcccagaac tcgccctcgg aggtggcgcc
     3301 ctcgcccagg ctcacgtagg tgacctcgtc accggcggcg ggcaggtgcc ccagctccgg
     3361 cgtgcggccg atgtagcgac cggcctcggc gcagcccacg gcggggatgc actggctgcc
     3421 cgtggggctg gactgggaca cgacgtgctc ggcggcgtag ccccagtggg agggcatctg
     3481 gcgtccgccc gacgcccggc actcggccga gccgacggct tggaggagga gctcgtatgg
     3541 gctgatgccg aggccgagca tcagcgccag gtctcggtag taggggaaga accagtcgtg
     3601 gccgggccgc agcgagcggg ccaggcccag cagcagggcc tcgtggccgg cgcccgagat
     3661 ctggaagaag accttgctct gcttctggag gctgatctcg cggtcgtcga tggcgcgcga
     3721 gacacaggcc agacggaagt cctcgatgac ctcctcgacc gcgaaccccc tgatgtcgct
     3781 caccagatcc gagtgtgcca ccccggccgg gcctcgcgtg ccgatgcgcg agcgcgacgc
     3841 ccgccgactg agcacatcgc gcagccgacg gcgcatcacc tgcgcagacg gcgcatccgt
     3901 ccgcggacct cctgaccgtt caggcgggca cggtcggcgg cggcgtgccc ggccgcgtac
     3961 cccgccgggt cgctggcggt gacccgcatg cggcgcaggt gggggaaggc cgacgccagc
     4021 tcgtcctcga cggcgacctc gcggctgcgc agcacgggca gcagcccctc gccgtgctcg
     4081 cgcacggcct cggacaccgt ggcggccgat gcctgccgga gccgctggcc gatgcgcgac
     4141 gcgtaggcga cgaggaacgc ctggcggaac gcccgcgacc gggtgcgctg gctggtgccg
     4201 gcctggagca tcgcccgcgt gccctgcacg agcagcgacg tgtagagcag ctccaccgtg
     4261 gcctggtcgt cggggaagcc gacgacggtg gcgacgttgt cggtggtgtc cagcacggcc
     4321 cggcaccggt tggccttggc cagctcggtg agcagctgga acttgggccg ggcgtagggc
     4381 gactccacca gcagcgtgcg accctcgggc cgggagcggc tcgcctcgcc cagcatcgcc
     4441 cggtcgatgg cgtggcgctc catgagctgc tgggccttgg ccgagagggc ctcggcctcg
     4501 tcggggtagc tggtggattc ggccttggcc agcagcgacc gcaccttgtg cagcacgccg
     4561 gggtcggggg cgccgccgcc ggcgccgtcg ggatcgaggt gagccatcac cccatcatgt
     4621 cggcaccctc cgacagcaac gccgtccgcc cgccgaccga ggaccccaga ccctgcccct
     4681 catcccggtg cgccggtaga cacagcacat gcgacgcctc tcccccaccg acgccgtgga
     4741 cctgttccgg accaccgaca ccctcggcct gccgctcggc ccgtcccagc cggccgcgct
     4801 gctcgaagcc atgggcagcc gcaaggactg ggaggacctg aaggtcttcg gcgcgctcgt
     4861 caccgtcctc accgacctgt tcacccacca caacgtccac tacctgagcg ggttcttcgg
     4921 cccgctcgag cggctgctgc gcgacagcgg tgccaacatc ggcttcgcac cggccgactt
     4981 ccgccgcttc gtcaccctcc tcgaatccat ctcgccccgg gtgatggcca ccgccgcggc
     5041 gccgccggac cgcgacgggt ggtgcagcct gtcgctgcac gccggcgcca acaccggatc
     5101 gctccacgcc gccggcgccg accccgaccg gctgctcgtc gtcgaggtga gcgacggcta
     5161 cccggtgacg tacgggtggg gtgagcacgg ccaccgcctc cacgtcgacg agatcgacgt
     5221 gctcatcgag tcggagtcca ccccgtacga gatcgccgac cccgagccca ccgacgtcga
     5281 gcgcgccatc gcccgccaca tccagggcct gatccccgac ggcgccacgc tgcagaccgg
     5341 catcggcgcc gtcccgtcgg tggtcgccgc gctgctggcc gagggcgacg gaggcgacta
     5401 cgggatccac tccgagatgt tcacgaccgg cctgatgaag ctgcaccagg ccggcaaggt
     5461 cacgaacgcg cgcaagggcc agtacgaggg cgtgtcggtc tgcaccttcg ccggcggcac
     5521 ccgcgagctc tacgactggc tgcacgagaa ccgggacgtc atcttcctcc cggtcgaggt
     5581 cgtgaactcg cccgagctca tcggcgccaa ccagacgatg atcacgatca acgccgccct
     5641 gtcggtcgac atccacggcc aggtcgtggc cgacacgcgc agcggcgagc agttctcggg
     5701 catcggcggc cacgaggact tcgtggccgg caccggcctc gacctgaccg accgctcgat
     5761 cctgtgcctg ccgtcgacgt accgggccgg cgaggaggtg cgctcccgga tcgtgccgtg
     5821 gttcgcggcc ggcaccgtca tcaccacgcc ccgccaccag gtcgacgtca tcgtcaccga
     5881 gcacggcatc gccgagctgc agggcaagac ggtgcaccag cgcggcgagg cgctcgccgc
     5941 catcgcccac cccgacttcc gcgacgggct cctcgaggcc gcccagcgcg cgtcgggcgg
     6001 cgcctccccc atgccctgac ggccgccgga gcgcgggacc cccttcggga cgagcgcgcc
     6061 gatcggtccg ttggttccac cgggcggaac gagcgaaccg aacctcgacc gacgcctccc
     6121 aggaacgagc gcgccgatcg gtcccgacgt tccatctggt ggaacgtggg gaccgaacgc
     6181 tgcgggggtc ctacccggcg cgtcgatggc ggcggcgcag atcctcggcg agccggcgga
     6241 gccagtcctc gcgactgggc agcaggtgcc cccggtggat cacgccgacc gaccagccgg
     6301 ccgcttcgat ccgggctcgc cggcgttcgt cctcctcgac cttcggcggt gggtggtagc
     6361 cgtctgagtc gtactcgcgg cacacccgct catccggcca ggcgaggtcc acccgagcca
     6421 cgaaccgccc ggcgtcgtcg cgcacctcgt gctgcgtgac cggtcgggcg aggccgaagc
     6481 cgatgatccg gcgcagcacg gtggcttctg caacggagtc gggcaggatc cgaccagccc
     6541 agctctcgag caccgtgcgc agggtgccgt gcccgatgcg gccgggttcg gcctccaccc
     6601 gccgggccgc ctcctcgatg tggcggacgg tggtgcgacg agcggtcacc gcctgggcgg
     6661 tcacctcgtg gagcgcggtc accgacaggt gcgcgctcgc atcgacgact gcccgggcga
     6721 gcgtggtgac ggggatccca cgacggcggg tgacgtcgat gcgctcgagc ggcgagcggt
     6781 gcagcacacc gagcgtcgtg tggctggtcg ctcccggcgg gagcgtcaga tgcggaggct
     6841 caggtggatc cacgtcgatg tcgtgcaacc acagcgcgct ctcgtgcgac gccacggcgc
     6901 ctgcatgcct gcacaccgcg gcggcgatgt cctgctgcgg gcacctcgtg cctgccacgc
     6961 ggtagagccc gcgaacgatc cgctcgatga tgcggtcgtc gcacaagcgg gcgacctgct
     7021 tgcggtccag gcccagtcgg gtgagatcgg aggtcgcaag gaggtggttc tggtcggcgg
     7081 cgtcctcgag cgcctgaccg agcccggtga tcaggtgcat ggtgacgatc ctggccggcg
     7141 gcactgacag gaactcgatc cgtccggtga gcgcagggga cgtcgggacc gatcggtcct
     7201 gacgttccat cggagggaac gaacgaaccg atcggtccgt tcgtcccgtg ttcaccgtcc
     7261 cgtggggagg agcggcggcc ggtcgggacg aactctccgc cgtgggcctc tctcgtcgca
     7321 ccttcatcaa gaccgctgcc gtggccggcg ccggcgctgc cctcggcggg ccggcgcacg
     7381 ccgacctgct gaacctgacc cgaccgacgt cgctcctgct cgacggtccg gcgccgaagt
     7441 tccccaacat ccagcacgcg gtggtcgtga tgatggagaa ccgctcggcc gaccactacc
     7501 tcggctggtg gggcgaccgg gaggacgtcc gcttcgacgc cagcacgacg ctgccgggac
     7561 gggagacctt cgacttcggc atcgacggcg agcagaactg ggccggccac ccgtacctgg
     7621 acccgggcca cagccacacc accggccggc gccagatcct cgaccacgac ggcgacggca
     7681 ccccggacgg gtggagcacc accgaggacg gcagcgaccg gccggggacg ggcaccgacc
     7741 gctacgcctc cagctactac accgccgccg acctgcccgt catcgaccgg atcgtccagg
     7801 acttcacgtc cttcgaccgc tacttcacct cgtggatggg aaacacgtac ccgaaccggt
     7861 actacatgca ctccgcccag gcccacggcc tcaacaacaa cgacttccca ccccagcgga
     7921 tcggtgagaa cccggcgtgg gcggccggct gggactggcc gacgctgtgg gaccagctga
     7981 actccagggg cgtcacctgg aagtactact tctcgaacct gcccgtgatc gcgctgtggg
     8041 gaccgcggta cctcgccaac acccggccca tcaccgagta ctacgcggac tgcgccgccg
     8101 gcacgctgcc ccaggtcgcg ttcgtcgacc cgttcttcgt cgctcccgag ggcctggcca
     8161 acgacgacca cccccacgcc gacatccggc tcggccagga gttcttcagc gacgtcacca
     8221 ccgcgttcat gcgctccccc aactggagca agggcgccat gttcatcaac tacgacgagt
     8281 ggggcgggtt cttcgaccac gtcgtgccgc cggcggtgga caacacggtc gacccgtacg
     8341 gcgcctacgc accgggtgag gtccccgact acaccgtcga gggaccccac accgccggtc
     8401 ccgatgactt cgggctcttc gggttccgca cgccggcgtt cctgctcagc ccctacgccc
     8461 gcaacaccgg tgtcaaccac cgggcgccgg cggggtcgat gccgccgaag tcgaagggca
     8521 aggggcaggg acggcgcagc gacagcgaac ccaccgtgga ccgcaccgtc agcgccccgc
     8581 tggaccacgt gtcgatcctg tccttcatct gcgagaactg gggcctgccc ccgaccgagc
     8641 agtgggaccc gggccgcagc cgacacgcca tcggcacgat gcgcgatgcc ttcgatccct
     8701 ccatcgccga ccccgaccac cccggcgacc ccgaggtcga cgtcgacgcc tacgagtacg
     8761 tcgcgcctcc ggaggcccgc accaccgcgc taccgatccc ggcgagctcg gtcagcgacc
     8821 tgtgggtcat ggccgactgg atcgaggccc agggctaccg catcaacacc cgtttcaccc
     8881 acgccctgcc cttcacccgc tgagcggcgt cgtctcggct cggttcaggc gttgtcctgc
     8941 aggaacgggg cgacccgctc cgggggacga ccgatgatgg cccggccgtg cagccacacg
     9001 acgggacgct ccatgagctc ggggtgctcg acgagcaggt cgacgaccgc ggtcggggtc
     9061 gtggcctgcg acgtgtcgac gccggccttc ttcgcgtcgc cgtggcggat caggtcggtg
     9121 ggcgggtcct cgaggccggc cacgatggcc tcgagcgtgg cccggtcggg cgggctgtcg
     9181 aggtagcgca cctcgtcgac gtcgacgccg agctcgtcgg cggtggacat cgcggcgcgc
     9241 gacttcgagc agcgcgggtt gtggaagatg gtaggtccgg taccgagcgt ggacatgacc
     9301 cgaacgtagc cccgccggcg cggcgtgcga ccctgggcgg gtgacgtccc cgcgcggcat
     9361 cgacgagccg cacccgagcc gcctggcacc cgacgacccg caccgggacc gcatcctcgc
     9421 cgcccaccgc gccgccgtgg acgccgggga acccggctac ctcgacccgg ccacggggct
     9481 gttcgtgctg accgccgtgg agctggccgc cgcgggctcg tgctgcgagc gcgggtgccg
     9541 ccactgcccg tacgtctgag ccgtcgcatc gacgaaggcc tcctcggcca cccactcggc
     9601 gtacgggtcc ggaccgaacg cctccaccac cggttgggtc tgggtggcct gggcccgcag
     9661 cgccacgagc ttgcggtcga gcgacgcgcc ggtgagcgac aggcgaagcg ccaggtcagc
     9721 caccggtctc ggcgcgggca gcgccggatc catgaacacg tcgaagcgct cgtgcagccc
     9781 tcggaaccgc tcgcagtagc cctcctcgag caccgcatgc aggacccgga cgcgccggtc
     9841 gctgcaccgg caggcggcct cgacccagct cgagacggcc cggtggtccg ggtggccggt
     9901 gacgccgtcg ggtccgaacg tcaggatcgt gtcgggcgcg acctcgtcga tgatcgccgc
     9961 caaccgaccg gcctgctcgg tggccgagca gcgggcgagc tcgccgtcgg ggagcccgag
    10021 ccagcggtga tcgtgcacgc cgagcgcggc gagtgccgcc gacgcctccc agcgccggac
    10081 cggcgccaac cggtccggtg gccaggcgac cgggtcgtcg gtgccgcgct cccccgccgt
    10141 cgccgacacg acgacgacgc gctgcccgtt gcctgcggcg atggccatga cgccggcgga
    10201 caggtaggtc tcgtcgtcgg ggtgggccca gatgcccagg accgtcccga gcgccgcgac
    10261 gtcgtcgagg tcgttcatcg gacggcgcag ccggagatgg ccgtgttgac cggcacgaag
    10321 gcggaccgct tcaggcgagc tcggcggatg gcgacctgcc ggaagccggc gacctcgatc
    10381 gtggctgccg tgtcccggca caggtcgcag ccctcgaaga cccagctcca cggccggcgg
    10441 atggcgtgct gcagccaccg gcgcgggctc caggacggcg ccgcgacgtg ctcgacgaag
    10501 cggaaccggc cgccgggtcg caggatccgt cggatctcgg cgagcacggc ctccgggtcg
    10561 tcgacggtgc acagcacgag cgaacagagc acctcgtcga cggaggcatc cgggatcggc
    10621 aggtcctccc cgtaggcgcc gatcacgatc aggtcgagac cccgtgccgc ggcacgcgcc
    10681 tcgagctgct cgtgcatggc ctcgctcggc tccacggcga ggacgcggct acccgggggc
    10741 aggtgggcga agttcgcacc cacaccggca ccgagctcga cgatgtcgcc ggcggcgatg
    10801 tcggcgaacg ccgaccgctt gtgctgccgg gtgatgtggt tcaggtagcg gtcgaaggtc
    10861 cggaagaacc aggcgttgaa ctcgctgcgg gcgccgtggt ggaaggtgcc gtgctccgtc
    10921 ggggcggggt cgggggcgag acggggatcg gtggtggtca cgaggtgctc ctcggtcggg
    10981 gcgtcgccga tcgacgccgc acccgcaccg tgacgagccc ccctcgcacc ccgctcgcat
    11041 cgtccttgca cgcgcgcgcc aggcccgtgc aaggcccccg cgaggtgcgg tccgcacggt
    11101 ggtcgacgag cgcacaccga ccaccgcagg agcacccatg cgaccacgcc aaccgaccga
    11161 gtccggagtc gtcgaccgcg acggcgtcgg catccactgg gagacctacg gcagcggcga
    11221 ggagaccctc gtcttgctgc ccccgtggat gatcgtccac agccgcatct ggaaggcaca
    11281 ggtcgccggt ctcgccgacc acttccgcat cgtcacctac gacgcccgag gctgcggccg
    11341 ctccgaccga ccgacggacc cggcggcgta cgggcccgag gcgcacacgg gcgacgccct
    11401 cgccgtgctt gacgcgacgg gcaccgagcg ggccgtgctc gtcgggaact cctacggggg
    11461 tctcctcgcg ctggtgctcg ccgccttcgc cccggagcgg gtggcggggg ccgtgttcat
    11521 cggaccgtcc accgacctct cgggcaccgc cgacgcgccc ctcacccgcg ccctgctcca
    11581 cttccacgac gagccggacg tcgaggaggg ctggggccgg tacaaccggc acgcgtgggc
    11641 ccgcgacttc ccgggcttcg tccgctggtt caccgagacg gcgctcgccg agccgcacac
    11701 gaccaagccc cgggaggacg cgctgtcgtg gggactcgac accaccgccg aggtcctgac
    11761 cgcgacggtc gcggcgcgcg ccggcgtccc accggagaag tccgcggccg cactgcgggc
    11821 gctgatcccg gccgtcacgt gccccacgct cgtcatccag ggtctgctcg acgagatctg
    11881 ccccccgacc ttcggccgtg acctcgcggg ggcgctcggc tccgagctgg tcgagatgcc
    11941 cgacgccgcc cactgccccc aggtgcggtt cccgatccag gtcaacgagc tgatccgctc
    12001 cttcgtcgct cgacgggtca gcaccgacac caccggcccg gtgggcgtcg tgtgcgatcc
    12061 aggtcacgcg ccggcaccgc gccgcacgcg cccgcctcgc gtgctgtacc tctcctcccc
    12121 catcgggctc ggccacgccc gacgggacct cgccatcgca tcggagctgc ggacgctggt
    12181 ccccgaggcc gagatcgact ggctggccca ggatccggtg acgcgcgtcc tcgacgccgc
    12241 cggcgagcgc gtgcacccgg cgagccgccg gttggccagc gagtccgcgc acctcgaagc
    12301 ggagaccgac gagcacgacc tccacgtctt cgaagccctg cgccggatgg acgagatcct
    12361 ggtcacgaac ttcagcgtgt tctccgatgt caccgcgtcc ggggagtacg acctcgtcgt
    12421 cggggacgag tcctgggagg tcgaccactt cctccacgag caccccgcga ccaagcgagc
    12481 gaggttcgcg tggctttcgg acttcgtcgg catcgtcccg atgccatccg gcggcgagcg
    12541 ggaggcgttc gtggccgccg actggaaccg ggagatgatc ggccacgtcg agcgccagcc
    12601 cggcatccgg gaccggtcga tcttcgtggg tgaccccgag gacgtcgtgc cgctcgggtt
    12661 cggccccggg ctgccctcga tcgccgagtg gacagcggcg cactacgact tcagcggcta
    12721 catcaccgga ttcgaccccg ccgagctcca gcacgacgtg ctgcgcgccg agctcgggta
    12781 ccgggacgac gaggtcgtcg tcatcgccgc cgtgggtggc tcgggcgtcg ggctcccgct
    12841 gctgcgccgc atcgtcgccg cccacccgct cgcggcgcag gagatccccg ggctgcgcac
    12901 cgtcgtggtg accggacccc ggctcgatcc aaacgccctc ccggacgtgg aaggcgtgga
    12961 gaagcgggcg ttcgtccccg acctccaccg ccacctcgcc gcgtgcgatc tcggcatcgt
    13021 ccagggtggg ctgaccacga cgatggagct gaccgccgcg cagcgaccgt tcctgtacgt
    13081 gccgctccag aaccacttcg agcagcaggt ccacgtgcgt caccgcctcg atcgccacgg
    13141 cgccgggcgg gcgctcgact acgcggccgc gacgccggag gtgatcgccg aggcgatgac
    13201 cgagcagctc cgccaggggc cgccccgcta ccagccggtc cgacccgacg gcgcccgcgg
    13261 tgccgcgtcg ctgctcgtcg acctgctgtg acgagccgac tgccagatcc atccaccgca
    13321 accaccagcc acaccgacag gagcaccaca tgaccatcac cgagcacccc acgaccaccg
    13381 gccgccagct gccggatgac gacgtcctcg ccgggttccg ggcgcgagcc cgcacgtccg
    13441 acgatcgcaa cgagtacttc cacgaggacc tcgccatcct ccgcgagatc ggctacctcg
    13501 gggccgccgt cccgaccgag ctcgggggtt ggggccacga cctgcgccag ctcgcccggt
    13561 cgcagcgccg cttggcgagc ttcgccccgg cgacggcgct cgccatgacg atgcaccact
    13621 actgggtcgg catcgccatg gagcacgagc gggtcggcga cacctcgctg cgctgggtcc
    13681 tcgagcgggc cgccgccggc gaggtgttcg cggccggaca cgccgaggtc ggcaacgacg
    13741 cacccgtcgt gatgtccacc gccacggccg agcgcgtgga cggcggctac cggttcaacg
    13801 gccacaagat gttcggctcg aacggtccgg tgtggtcgtg gctcggcgtc cacgcgctcg
    13861 atgccagcga ccccgagcga ccggtggtcg tccacggctt cgtgccccgt gatgccgacg
    13921 gcgtgacggt ggtgcccaac tgggacaccc tcggcatgcg tccgtcccag agctacgaca
    13981 ccatcctcga cgacgtcttc gtgcccgacg atcggatcgg ggccatcacc ccggccggcg
    14041 acgagtcgga cctgttcctc ttcgccatga acctctggtt cgtgggtcag tgcgggagcg
    14101 tctacctcgg catcgcggac cgtgccctgg agctggccgt ccggtcagcc acctccaaga
    14161 cgtccgtcgc catcccccgt ggcacctacg cccacaaccc catggtccag caccaggtcg
    14221 ccgagatgta cctggagctc gacgccgccg atgccacggt gcgggccttc ctcgatgacg
    14281 tcgtcgacgg catcgaccac ggcgacctgt ggggcgccaa gatcgtgtca gcgaagtggc
    14341 gggcggtgga ggccgccaag cgcgtcgtgg accgggccct cgacgtggcc ggcggaggcg
    14401 gcatggtgcg cggcaacgag ctcgagcggc tctaccgcga cgtgcgctgc ggcggtttcc
    14461 accccaccaa cgccgcgctc accttcgaga ccgccggcaa gaccgtgctg ggcatcgacc
    14521 cctcgggtcc tcgctggtga tcagccctcg atgagctcga acgcagccgt cgccacgctg
    14581 gcggcggctg cgtcatcgag cgtcgcgccc tcgacgagca gctcgtccag ccgggcggag
    14641 ccgaggcgct gctcgagctc ggcgcgaacc gccgccaacc ggtcgacgtc gtcgcccacc
    14701 acctcgtgcc cgacccccgg ggccgtcacc gcaccgagca ggagggccgc ctgctcgtcc
    14761 aggccctgcc cgcacagcag ctccgtgacg gtccgcagca tcgtgcggag cggcgaccgc
    14821 acccccgccc gcaaccagag cggcagcagc catcggtagt gcccgacggc agcccggacg
    14881 tcgccggcac gagcatcgag ggaggccagc gacgcaccgg ccacgccctc gaccagctgg
    14941 ccgccaccgg ccctcgccag ctcgaccgcg ctccgcagcc gacgtcgcgc ttcgggcgga
    15001 tcgtcgtcca ggagcacctc gccggcgccg taccagcacc aggcggccgc gatggcaccg
    15061 gctccagggg cgacctcgtc gagcaaccgc tcggcatccg cgaccgcttc cggtgcgtgg
    15121 gcgtaggact cggcgatggc caacgtcgcc ccgtcctcgc cctggcgcag cgggtcaccg
    15181 acggcgtcgt cgagtcgcag cccggcgcgg aacccctcca ccgcatcggc gagcgcgcca
    15241 tgggccagcg actcggtggc ccgggcgccg atgagctcgt aggtcggggc caacccgagc
    15301 tccgcggcaa ccgcctcggc tcgatccagg aggtgtccca tgcgctcgag gtcgcccgac
    15361 ttccacgcac cgagcgcggc caccgcgtag gcgtcgacgg cgagcgggtg gcccgcagcg
    15421 gcgcccaggt ccccggccgg ctcgccccag gccatcagct ccgggagcat cccgtgcatg
    15481 gccgggtccc gtacggccac gacgatgcgc agggccagat ccggatccct gcgcgccgtt
    15541 gccgcggcca tcgcctggcg gaggtcgggt accagggcgc ggaccgcctg catcggcgca
    15601 tcgtcgcggg cggtgcggag cgccgagctc gcggcggaga cccgcgcggc gacgcggtcg
    15661 gcgtggcgac gggcgagctc gtctcgccgg gtgccctctg accgttcggc caggaagctc
    15721 cgcaccacct gcagcaaccg gaaccggccg tcgtcgcggt gcaccagcga tcgctcgacg
    15781 aggtcggcga ggcgatcgtg cacatcgaac gcgctccgat cggccaccgc ggccacgtcc
    15841 tcggcatcga aggcgccggc gaacgtggcc gccgcagcga ggaccagctg gtcctcggcg
    15901 gcgagcatgc ggtgcgacca cgcgagggcg gcgtcgaggg agcggtgtcg cggcaccgtg
    15961 cggggcccgc cccgcagcac cgcggtgctg tgcgccaggc cggcgatcac ctcctcgagc
    16021 gagagcgatg cgagccggga ggctgcgagc tcgatcgcca ggggcagccc gtcgagtcgc
    16081 tggcacagat ccacgacgag gggccgggtg cggtcgtcga gggcgaacga cggcgaggcc
    16141 gcgacggcac ggtcggcgaa cagcaggact gccgggctct cctccccgct cgcgcccagg
    16201 gggtcgacgg ggaacagccg ctcaccgtcc acggcgatcc gctcccggct ggtcgcgagc
    16261 agcacgaccc gctccgtcga gcggagcagc tcgtcggcga cgctcgcgac gccatccagg
    16321 acgtgctcgc agttgtcgag gacgaggagg ctccgctcgt ggcggatcac ctcggcgacc
    16381 cgctcggtgg tgctcacgcc ggggcggtcc tcgaccccga ccgtggccgc caccgcagcc
    16441 gggacaccgt cgtcgtcgac ggcggtgagg tcgcagaagc gcgccccgtc ggggaacgac
    16501 tcgaccacga ggtggcacag ctcggtgacc agacgggtct tgccgatccc accgggtccc
    16561 acgagcgtga tcaggcgctc gtgctggacg agttcggcca gcacacccag cagctgcgct
    16621 cggccgacga aggacgacac gggacgggga aggggtcgcg ccaccgccgt cgcgcccgct
    16681 gcgggccggc gctgcgggac gggatcaccg tcgaggaggc gctcgtggac cgagcgcagg
    16741 tgcgcggacg gttccacacc cagctcctcg gcgagctggc ggcggtaggc gtcgtacacg
    16801 cgcagggcgt cggctcgccg gcccgaggcg acgagcgcgt ccatcagcag ctcccggggt
    16861 cgctcccgca agggctcacg agccgcgagc gcttcgaggt cggcgacggc atccgcaccg
    16921 cgcccgaggt cgaggagggc ggcgaagcgc tcctccaccg cccggaggcg cagctcctcg
    16981 agccgctccg cctcgatgcg accggcgtcc aggtccacca ggtcctcgaa ggcctcgccg
    17041 cgccacgacc ggagggcgcg gtcgagggcc tcgagcgcac cttcggggtc gccgacgcgt
    17101 tcggcagccg cccgcgcgac tgcgcgctcg aaccgggccg cgtcgatccc tccctcgggc
    17161 aggcgcagca cgtagccagg cggcgtcgcc tcgagctcga tgtccggtat gtgctggcgg
    17221 agccggaaga tgtgggagtg gacgagggag gcggcgtcgg tcggcaaccg gacgggccac
    17281 agcacctcgg tgagctgatc gatcgagacc accgcgccgg cccgcagggc gaggaaggcc
    17341 atcaggcgac gttgcagccg cccccggggc gtgacgtcac ccccggcagc gtcccggacc
    17401 cgcagcggtc cgagcacctc gactgcgatc gcgttcatcg tgctcgcccg tcctccgacg
    17461 ggcgagcgta acgaagggct catcgggccg gtgcgtgtgg gtcactcctc ccaggagcgg
    17521 aagccgacga aggacgcctc gtggcggaac ccgaggcgct cgtagagccc acgggcgggg
    17581 gctcggtggt cctggacggt gagcgacatc cgtccgaggg gcgatcgcgg cagcaggtcg
    17641 cgcgcgatgg cggcgacggc ctgccggccg agaccgcgtc cgtgctggtc ggggtgcacg
    17701 acgacgaagt cgaggcaccc ctcgccatcg tcctgcaggt gaccggacgc gtaggccgcg
    17761 accccgccgg cgccgtcgtc ggcgaccagg gtcacccggc tgccgtcctg ctggccggca
    17821 acgagctgat cggcggtggc gtaggtgccg gggaactcgg cgtcgtgcag gggccggatg
    17881 ccctcgaggt cgtcggtcgt ggccgggcgc agaccggtga cgtcgatgtc gccccacgtc
    17941 cccacgatcg cttcgtcgac ggagagggcg tggttgacct cggtcggcca ccacccccgt
    18001 gccgcggcca gcgccgcgac ctgctggttg gcgaggtcgc ccgacacctc gtggcgggtg
    18061 atcgaggacg gcacctgcgc gagcgcggcg tcgagcagct ccccggcgag ggggagccag
    18121 cggtcgtcgt cgtgggcgac ccacgggccc atcacccagg agcgtccgac gtcgtcgtcc
    18181 cactccacgg cgacggcacc aacgatgcga tcgtcctcgc gggcgatccg ggcggtggac
    18241 gtccatgccg gctcgaggcc ctccagctcg gcggtgaccc cctccggatc ggtgccgagg
    18301 taggggatgt tgcggtcggg ccggcgctgc tggtcgacga tgaggtcgac gacggcggcg
    18361 acctcctcgg gggcgggcgg ggtgagcatc ggcgaacgct agatccggcg gccggtggcg
    18421 aggaggtgcg ggccgacgcc gagcagctcc gggacggatt cgagggcgcg gagcgtgtcg
    18481 agcagcaggg tgcgctcggt gtcgtcgtcc cagcgctcgt ccaggtcggc gagcgcgaag
    18541 gcgacgccct cgagcgacac cagggactgc acctcgaacc ccgcggccgt gacctcgtcg
    18601 cggagctcgt ccggccggtg ggtgtagccg gtgaagcccg cctccacgat cggggggagg
    18661 tggccggtgc gctcggcctc gtcgacggcg tcgaccatcc ccgggaacag gaggtggaac
    18721 cggttcgtca ggatgccgtc catccgggtc gcccaccggc tgatggcggc ggcgtgcagg
    18781 atgccgccgg gtcgcaggat gcgggccgcc tcctgcagcg cgcggccacg gtcgccggcc
    18841 tggacgaggt ggtacagggg cccgagcagc agcaccacgt cgacggaggc gtcgggcagg
    18901 tcgagggcac gggcgtcacc caccgcgctg tcgacccgct cgccgtgccg ggcccggacc
    18961 tgggcgacgt ggtgctcgac cacgtcgcgg tggaccacgt cgtagcccgc ctcgaccagc
    19021 cagtcggtgt agcgccccgg gccgccaccg aggtcggcca cgaccgccgg cgggggcggg
    19081 agggtccggc cgaccacctc gatcgtgcgg gccagctcca cccgcccgag gccgaaggcc
    19141 agccggtcgc gctccttgtc ctgcgcgtag tacgcccgca tggcccccag gtcgtccgcc
    19201 atccgtgcat gctgccgccg cccgggccgc tcggccagcg cgttccgccg gcggtcgtag
    19261 cctcacgacg tgcccggcac gatcgaccgc ctgcccgacc tcgagatccc gggccaccag
    19321 ccgtcacggc ccgacggcgc cgaccacccg atgcgggtga tgacgcggcg ggccgccggt
    19381 ctccaggcac cgccgtggga cgcacgcgcc cggcacgagg tggcggcgct gttcgacggc
    19441 ctggccccgg agtggcacac ccgcacatcg ccggagcggg cccgggtcgt cgcagacgcc
    19501 ctcgaccgcg gggaggtcgg ccccggcgcc accgcgctgg agctcgggag cgggatcggc
    19561 acctacacgg cgaccttggg cgagcgcttc ggcgaggtgg tcgccatgga cctctcgctg
    19621 gagatgctgc gcctcgcggc ggacggaccg gggcaccggg tgctcgccga cgcgcaccgc
    19681 ctgccgctca ccgacgccag cgtcgacgcc gtggtgctgg tcaacatgct cctgttcccc
    19741 gaggaggtcg atcgggtcct gcgtccgggc ggctgcgtgg tctgggtgaa ctccagcggt
    19801 gaggccacgc cgatccacct gctgcccgag gaggtcgtcg acgcgctccc gggcgcgtgg
    19861 aatggcacgt gggcgcgggc cgggaccggg ctgtggacgg tgctgcgtcg ggactagccg
    19921 gcgacggccg acacgagcgg agcgtcgctg cccacatcgg cgaagcgctc ggccatgtcg
    19981 gcgaagaaca cggcgttggc ggccgatcgc caccgggcgc cgcgcaccat cccggccgcc
    20041 cagcgggggt cgccggcgag gggggcgatg gcgttgtcga gccaggcctt gccgtggtgg
    20101 ggatcgacgt cgcggtgctc gacgtagaag gggtaggccc cggcgggggc gtcgaggcgg
    20161 tcgaaggcct tcacgacctg gcggcagcgg ggcccggcct gcagctcgag caggccgagg
    20221 gcgccgacca tctccggctg cagcacccgg ttggtggcga ggatgccgcc gagcgaggag
    20281 cgcagcagcg cgctggtggg cagctccgag cgcggcacgt gcggcatgtc gatggcctcg
    20341 cacaggtcgt ggtggagctc ggtgtggatc tcgtccagct cgccgcggcc catctcgtcc
    20401 cagtagttgc gagccagctc gagcttgggc tcaccccgca gcccgatctg gcaggcggcc
    20461 acgaggtcgt cgaagccggc gtcaggtcca ccctccaggg cgaggaagtc gaccagcccg
    20521 tcccaggtcg cgtcggccgc gacccactcg tagatgcggg gcacgaggtc ctggcgggcg
    20581 atgacccgca tcgtggcgac cgcgtcgtcg tcaccgtcgg tggcgtcgcc ctcgatcatc
    20641 gcgtcgagct cggccaggac gtcctgctcg aggtgggtct tcagcgcagc gactgcgggg
    20701 tggtgctgcc agcggaccgc gtcgccgacc cggtcgatgt cgccgacgtg gaggtcgtgg
    20761 atgacctgca gggtcagcag acggtcgcgc tcgtccaccg gctgggcgtc ggccagcgcc
    20821 gagagggcgt cttcgccggt gcgcagggca cgctccagct tctcgacgag cgaccggggg
    20881 atcgccgtcc tggcaacggg gggcatctgc gtaccgatac cgcgaagtcg gaggaaccag
    20941 acctcgctcc gtgttccgtg gccgctgcca gaacgtccgg accgggccgg ggagcgctac
    21001 gcgggcgcga gctcgtccag ggcggtggcg gcgtagaggg cgacgccgac ggccatggcg
    21061 tcctcgtcga tgaccatgcg gttggagtgg ttcggggccg cctggtggaa cgagacgccg
    21121 ggcgggcacg tgccgaggaa cgccatggcg ccggggacct gctggagcac gtagctccag
    21181 tcctcggcgc ccatcaccgg gttgggcatg tccacgacca ggtcggggct cacgatgcgg
    21241 cgggcgacgt cgcccaccca ggcggcggcg tcggcgtcgt tcacggtgac cgggaagccg
    21301 tcgtcgatgc ggaagcggac ctccatgtcg tgggccgccg cgatgttcgt ggcgatccgc
    21361 tcgagctcgg cgtggatcag ggcgcgggtg cgctcggaca cggcgcggat ggtgccgacg
    21421 aggcgggcgg tctcggggat gacgttgttg gtggaaccgg cccggatgct gccgaccgtg
    21481 accacggcgg ggtcgaacac gtcgacccgc cgggtcacca gggtctggaa cgcgccgacc
    21541 atggcgcagg ccaccggcac cgggtcgttg gcgtggtggg gcatggcggc gtggccaccc
    21601 cgaccgacga cctcgacgac gaactcgtcg gccgacgcca gcagcggtcc ggccttggtg
    21661 gcgaccatgc ccgaggggaa gctgggcgac tggtggatgg cgaaggcgcg cgtgacgtgg
    21721 agcccgtcga gcaggccctc ctccagcatg tgccgggccc cgtggtggcc ctcctcgccg
    21781 ggctggaaca tgaagacgac cttgccggtg aggtcgtcgg cccgatcggc gagcaggcgg
    21841 gcggccgacg ccagcatcgc cgtgtgggcg tcgtggccgc aggcgtgcat cgccccgtcg
    21901 acctcggacg cgtagtccag gtcggtgtcc tcaggcatcg ggagggcgtc catgtcgccg
    21961 cgcagcagca tcgtgccgcc ggggcggccg gtgtcgagcg tggcggtcac cgaggtcacc
    22021 ccggtgccgg tgcgcacgtc gaagccgagg tcgccgatcg cctccagcac ggccccctgc
    22081 gtgcggggca ggtcgttgcc gatctccggg tggcggtgca ggtcgcggcg cagggcgacg
    22141 gtgtcgggct gcagggcccg ggcggcatcg agcaggtcag cggtcacggc tggaccctag
    22201 gcagcacggc gtgacgcggg cgtccggact gggcaccggc gcgaagggga atcgattcgt
    22261 ccatgaccgc agactccgac gacagcatcc ccgagcaggt cgaggacggc gagctcgagg
    22321 atcccatgga cgacgaggac gaggacgagc acgagtccgt catcgatcgc atgcgcgggt
    22381 cgggtgtcgg ggtcgaggac cccgacatcg tgggcgacgc ccaccccggc accgtcgacg
    22441 ccgagatcgc cgaggacgag cccccgcagg tctgagccgt tcgttgcgct actgcaggac
    22501 cttgatggcc gcgtcgtagt cgggctcctg cgcgatctcg ggcacgagct ctcggtgcag
    22561 gacggtgccg tcgccgtcga ggaccaccac ggctcgggcg gccacgccgg ccagcttgcc
    22621 gtcggtcatc cgcacgccgt agtcgtcgag gaagctggag cggaacgtgg agccggtgac
    22681 cacgttctcg atgccctcgg caccgcagaa gcgggcagcg gcgaagggca ggtcggcgga
    22741 cacgcacacg acggtggtgt cgtcgaggtc ggcggcccgc tcgttgaacg tgcgcaccga
    22801 cgtggcgcac acggccgtgt cgatgctcgg gaagatgttg agcacgacgc gctgaccggc
    22861 gaggtcgtcg gtgctgaccg gcgtgaggtc gctcttgatg agctcgaagg cgggcgcctt
    22921 ggctccctcc tccgggagct cgccgttggt gtggacggca ttgccgccga gggtgatctc
    22981 tgccatggcg gccgttctat cggatcgccg gccccgtccc tcctcggctg cgggctgcgg
    23041 atcgctcgat aacagtcgtt acggtgggaa cggtgacccc gcccgcccag caccgggagc
    23101 gcatccaggc gcggctgacc gactccgacc ggtacccccg gtgggtgctc ctcaccgcgc
    23161 tggccggcat gttcgccacc accttcccgg tcaccctgct cgctgtcagc atcccgacca
    23221 tcgccgacga cttcggcgcc ccccagacgc tggtcgcctg ggtgatctcc ggcccgctgc
    23281 tggcgtccgc cgtggcgctg ccgatcctcg gcaagctcgg cgatctctac ggccagcggc
    23341 gggtgttcct catcggcttc gtcgtgtcca cggtcgtcac cgcgctcacc accctggcgt
    23401 gggacccgta ctcgctcatc gtcctgcgcg gtctcgccgt cgtggtcggc gccgccacca
    23461 tcccgtcctc gatggcgctg atcaactccg tgcacgcgcc ggcgcagcgg gccaaggcga
    23521 tgggctggtg gtccctcgtc gccgccggct ccccggccat cgggctcatc atcggcgggc
    23581 cgctcatcga tgccatcggc tggcggccct tgttcggcgt gcaggccgtc ctctcggtca
    23641 tcccggtcgt ggtcgcgtgg ctcgtgctgc gggagaccac gcctcgccgc gacgtgggct
    23701 tcgacgtgcc cggcgccggc gccctggccc tcggggccgg cggcatcatg ctcgccctca
    23761 accagctgcc gagccggggg ttcgacccgc tcgtcgccgg cgccgccgcc gtcggcatcg
    23821 ccggcctggc cgccttcgtc gccgtcgagc ggcgggtggc gtacccgctg ctgcccctcg
    23881 agctgttccg gcggcgcaac ttcaccgcgt ccatgctcgg ctcgctcttc tcgggcgccg
    23941 cgtacatggg cggcttcgtg ctcgctccgc tgctgctgca gtcggtgttc ggctactccg
    24001 tcgccgccag ctcgttcatc atgctgctgc gcccggcgtc gttctcggcg acgtcgccgg
    24061 tcggcggatg gctggccacc cgcatcggcg agcgcccggt ggccgtcttc ggcgccaccg
    24121 ccatcgccgc ggccctcctc gccctgggct tcggtgccag cgaggagctg gtggtgctgg
    24181 tcatgggcgc gctcgtgctg cagggcatcg gcaacggcac cgcccagccc acgctcaccg
    24241 cgaccctgtc caactccgtc gaggagcacg acctcggcgt cgccgcggcg gcacagcgca
    24301 tgatgttcca ggtgggcgcc gcgctcggca tcaccgtgat gatctcggtg tacggcggca
    24361 ccggcgaggc gtcggacttc ttcaacggtt acgcggtcgg ctccgtcctc gggatcgccg
    24421 ccatcgtgtt ctcgtggttc atccgctcca cgccacgggg ccccgctgcc gacgacgagg
    24481 tctccgagga gaccgcggtg ggggcgacgc cgcccgccgc cccggtctcc gtcacggccg
    24541 gcgactgagc accgacgccg ttccgggacc gggacggtgg cggggcatca gccgagatcc
    24601 acgaagacgt gcaccgtgta gacggtcccg tcgcgcgcga cggcgtggcc ggtgccgatg
    24661 cggtcccacc gggggttcag gatgttggcg cggtggctgg gcgagcgcat gtagccgtcg
    24721 tggacgctgc cgatgctgga tccccgaccg acgttctcgg ccacggccac ccagggcacg
    24781 ccgtcgaggc cggagctgag ggtggagtgg gacaggttgc cggcctcggc gaggcggttc
    24841 gcccagtcct gggccttcag gcgcacggcg aggtcgtcgc ccagcgcgtg cacgccgtgg
    24901 gcgcggcggc tctcgttgac gtgggagcgg accgactcct ggtccggcgt cgggtcgcac
    24961 gccgccaggc cgagcacgag gagcggtgcg gcgaggacga gcgcgatgcg cttcgtggac
    25021 cagcgtcgac ggtcgggcgg ggcggtgtga gcgtgccggg gcgcgcgcgt ggtgagcgtc
    25081 atggggaacg gactcctcgg gatgtgcatg tggggttgca ccgctgcccg tgtccctcgg
    25141 cggcgaccgt agcccacccg ccgtcatgaa actgcaaccc aacggcaata gatccgcgcg
    25201 ctgtccgaaa tgtggtcgcc agctagggct gcagcaccac ggcctcgtcc gggccgaggc
    25261 gcccgctgaa ggggtggccg gcgccgagcc cgttcgaccc cacgaccacg gtgccggtga
    25321 ggtcggtgac gtcggccggg gcgtcgccga agttcaccgc cacgatgcgc tcgtcgtcac
    25381 cgtggacgcg tcggtaggcc agcacgtcgt cggtgcgggc gaacagctcg aggctgccct
    25441 gctgcagcgc cggtgaggcc ttccgggcgg cgaggagctg gcggtacagg gccagcaccg
    25501 agtgggagtc ggcccgctgg gcctcggcgg accgggtggc ggcctcgggc gggaacggca
    25561 gccacgggtc ctcgccccag ctgtggggct cctcgacggt ccaggggatc ggcgcccggc
    25621 agccgtcgcg ccccccggga tcgacctcgg tgccggcggg gatgaccgcg tcctccaggc
    25681 cgagctcctc accctggtag aggaacggcg tgccccgcac ggtgaggagc aggacggcgg
    25741 cggaccgggc ccgggcctcg ctgcccccgt agcgggtgcg gtgtcgctcg ttgtcgtggt
    25801 tcgacagcac gtaggacggc caggtggcct cgtggtcggc gtaggcgtcc tccatgccgg
    25861 cgatcaggag gtgccaggcg tcgcggtccc acccggcgcc gacggagacg aagttcaccg
    25921 tgcagctgac gccgtcgccg tccccgcagt aggacgcctg ctgctcgagg tcgccggtga
    25981 cgacctcgcc gaccacgagc cgctcgacgc cgggcgggtc gaccacctcg cggatccggc
    26041 ggatgagcgg catggccgtc tcgtggtggg tcagcacgac gtggtcgatg ccgaccatgc
    26101 gctcgggcac gtccttgggg tccgggtcct tgccgatggc gttgatgacg tcgacgcgga
    26161 agccgtcgac gccccggccg agccagaagc gcagcacgtc gtgctgggcc gcctccaggt
    26221 cggggttgga ccagttcacg tcgggctggt cgggcaggaa caggtgcagg tagtactggc
    26281 cggactgctc gtcgagggcc caggccgact ccttcgtgaa cgacgcggtc cagttgaacg
    26341 gcgggccgcc gtcgggggcg gggtcggccc agatgaacca gtcgcgcgtg tcggactcgg
    26401 gatgggacac ggcctccacg aaccacgggt ggtcgctgga gacgtggttc ggcacgaagt
    26461 cgacgatgag gcggatgccg cggtcgtgca gggcggcgag cagcgcgtcg aagtcctcca
    26521 tggagccgaa gaccgggtcg accgcgcagt ggtcgctgac gtcgtagccg ttgtcgcgca
    26581 tgggcgactc gtagaacggc gacatccaca cgcagtccac cccgaggtcg gccaggtggt
    26641 cgacgttggc gaggatgccg ggcaggtcgc cgacaccgtc gccgtccgcg tcggcgaagg
    26701 agcgggggta cacctggtac accaccgcgg tcttccacca ggggaggtcg tcggtggtct
    26761 cggtcggctg gggcggggcc acggccggac cctaccgatg cccgcgagcg ctgccgccga
    26821 cgcgccacac tcgtccggcg tggagcagca cgcgccgggg gcaggcgccg ccgagggagc
    26881 gcggatcgtg cgcccccgct ggcgggcgag gctggtgcgg ggcttc
//
LOCUS       DATISU010000189         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_847854, whole genome shotgun sequence.
ACCESSION   DATISU010000189 DATISU010000000
VERSION     DATISU010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847854"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VLR27_07765"
     CDS             <1..802
                     /locus_tag="VLR27_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSP03380.1"
                     /translation="DQSIVSPAMDITGSSSVITGGASGLGEATARLLAEKGQHVVIVD
                     MNEEKGEAVAKDIKGVFVKADVTNTDEVIAAIEAAKEMGPLKTMVTAAGIGFATRTIG
                     KDGQYDSAHDLNIYKKVVEVNLVGTFNCVRLGATAMSQNEADEDGQKGAIVTVTSVAA
                     FDGQIGQAAYSSSKGGVVGMTLPIARDLSAAGIRVNCIAPGLIDTPIYGEGEASEAFK
                     AKLGESVLFPKRLGKSSEFATMAAELLTNSYMNAHVVRVDGGIRMPPK"
     gene            957..>2372
                     /locus_tag="VLR27_07770"
     CDS             957..>2372
                     /locus_tag="VLR27_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03381.1"
                     /translation="MSHRSRAAALVFTCVALVGASTTTASADEVDDLYAGANAVTATL
                     SVLGTDLGSFAGTSAIAEKTPLVEVASFGFQGPDGTPGIGITKAVADTLGAAVSDPGD
                     AGQACGTPDLAALPVADLLSALGVCSSSQAALPDLLAPQALGAADAGGIALNGQALAD
                     VIFDLLLDPILAQLDGVVVQVQGALAPVESALAAVCDELPQELSAVGLAEQADSAGLP
                     VTDIIRALPEGEQILESLESDCLLSLDTLVDVVAAVPALAEGVVRDTLLAALDQDLLA
                     ITLGSSSSSISPVDGALAASSQLQALDVSTLSLQFLLDAVEDLVTVQVQGVLDEVLAA
                     VPAELGAVTSQIPALGDVLGPVLAQLDGLVDDAPLLQVTVADSLADARSLPGSDSVDT
                     SRSTAGGVTVKISPAIAGLLGIPADITVPAGQRQVIAEGTPLESVISVGAVEQITEVL
                     GASEVQTAGARVTATEVALLTG"
BASE COUNT          345 a          953 c          740 g          334 t
ORIGIN      
        1 cgaccagtca atagtctccc cggccatgga catcaccggt tcttcatctg tcatcaccgg
       61 cggggccagc ggcctcggcg aagccaccgc acgcctcctg gccgagaagg gccagcacgt
      121 cgtcatcgtc gacatgaacg aggagaaggg cgaggcggtc gccaaggaca tcaagggcgt
      181 cttcgtcaag gccgacgtca cgaacaccga cgaggtgatc gcggccatcg aggcggccaa
      241 ggagatgggc ccgctcaaga cgatggtcac cgcggccggc atcggcttcg ccacccgcac
      301 catcggcaag gacggccagt acgactcggc ccatgacctg aacatctata agaaggtcgt
      361 cgaggtcaac ctggtcggca ccttcaactg cgtgcgcctc ggcgccacgg cgatgagcca
      421 gaacgaagca gacgaagacg gccagaaggg cgccatcgtc acggtcacct ccgtcgccgc
      481 gttcgacggc cagatcggcc aggccgccta ctcgtcctcc aagggcggcg tcgtcggcat
      541 gaccctcccg atcgctcgtg acctctcggc cgccggcatc cgcgtcaact gcatcgcccc
      601 cggcctcatc gacaccccga tctacggcga gggcgaggcc tccgaggcgt tcaaggccaa
      661 gctgggcgag agcgtcctgt tccccaagcg cctcggcaag tcgagcgagt tcgccaccat
      721 ggccgccgag ctgctcacca actcctacat gaacgcccac gtcgtgcgcg tcgacggcgg
      781 catccggatg cccccgaagt agtgagctga cgctccagct gatcctcgga tcgacgaacg
      841 ggccccggcg acgagctggg gcccgttccg cgcccggcga ggactcgcgg gaccgtcacg
      901 gaaacgtcgc atccgctggg caggaggcac gtgcctccgg ggcgaacgtg tgtgccatgt
      961 cacaccgctc gcgcgctgct gcgctcgtct tcacctgcgt cgctctggtc ggcgccagca
     1021 ccaccaccgc ctcggctgac gaggtcgacg acctctacgc cggcgccaac gccgtcaccg
     1081 ccacgctctc cgtgctgggc accgatctcg gcagcttcgc cgggaccagc gccatcgccg
     1141 agaagacccc gctggtcgag gtggccagct tcggcttcca gggccccgac ggcacccccg
     1201 gcatcggcat caccaaggcc gtcgccgaca ccctcggcgc cgccgtcagc gatcccggcg
     1261 acgccggcca ggcctgcggc acccccgatc tggcggcgct gcccgtcgcc gacctgctga
     1321 gcgcgctcgg cgtgtgcagc tcctctcagg ccgccctccc cgacctgctc gctccgcagg
     1381 ctctcggtgc agccgacgcc ggtggcatcg ccctcaacgg ccaggcgctc gccgacgtga
     1441 tcttcgatct gctcctcgac ccgatcctcg cccagctcga cggcgtcgtc gtccaggtgc
     1501 agggcgcgct cgccccggtc gagtcggccc tcgccgccgt ctgcgacgag ctcccccagg
     1561 agctgtcggc ggtcggtctg gccgagcagg ccgacagcgc cgggctcccc gtcaccgaca
     1621 tcatccgggc gctccccgag ggcgagcaga tcctcgagtc cctcgagtcc gactgcctgc
     1681 tctcgctcga cacgctcgtc gacgtcgtcg cggccgtccc cgccctcgcc gagggcgtcg
     1741 tgcgcgacac cctcctcgcc gccctcgacc aggacctcct ggccatcacc ctcggctcgt
     1801 cgtcctcttc catctcgccc gtcgacggtg ccctcgcggc cagctcccag ctgcaggcgc
     1861 tcgacgtcag caccctctcg ctgcagttcc tcctcgacgc cgtcgaggac ctcgtgaccg
     1921 tccaggtcca gggggtgctg gacgaggtcc tcgccgcggt gcccgccgag ctcggcgccg
     1981 tcaccagcca gatcccggcc ctcggcgacg tcctcggccc ggtgctcgcc cagctcgacg
     2041 gcctcgtcga cgacgccccg ctgctccagg tgaccgtggc cgacagcctg gccgacgccc
     2101 gctccctgcc gggcagcgac tcggtcgaca ccagccgcag caccgccggc ggcgtgacgg
     2161 tcaagatctc cccggccatc gccggcctgc tcggcatccc cgccgacatc acggtccctg
     2221 ccggccagcg ccaggtcatc gccgagggca cgcccctcga gtcggtcatc agcgtgggcg
     2281 cagtcgagca gatcaccgag gtgctcggcg cctccgaggt ccagaccgcc ggcgcccggg
     2341 tgaccgccac cgaggtcgcc ctcctcaccg gc
//
LOCUS       DATISU010000190        16766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_195531, whole genome shotgun sequence.
ACCESSION   DATISU010000190 DATISU010000000
VERSION     DATISU010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 16766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16766
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195531"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(436..3306)
                     /locus_tag="VLR27_07775"
     CDS             complement(436..3306)
                     /locus_tag="VLR27_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HSP03382.1"
                     /translation="MEVDGPIVGVSARGLTSGEAAERLARDGPNDIVPVHRGQRLKRL
                     LGPFADPMVALLLVAAPTYLLIGETTDALVALVALGPIAAVGWLLESRAERTLDQLRR
                     LAARTAAVVRDGVDQTVPASSLVVGDLVRVLEGDVVPADAAVTELTQLLVDESSLTGE
                     SLPASKQVEAVGDEAMIWAGTTVLSGRALARVTGTGRATRYGQIGALVAAGRSPLTPL
                     QRGLVRLVRGLTVVAGVFCIAVVAATLLHGDGWGDAVIAGVSLAIAAIPEEFSMIYTL
                     YLALGAWRLAQDRALVRRLPSVETLGSTTVICTDKTGTLTHGRLAVAGLWTTDGAGSD
                     GGSPSQAEVDLIEAAVLACEPDPYDPLDVAIVDHARCHDVDVARLHGGGLARDWPFDP
                     TDKYLTHVWRSGEDDTMWVAAKGAIEGILARSHADGAQRAAAAQANTAFSESGMRVVA
                     VAAGRANVTATSRSDDEAHLRLLGLVAFSDPLRDGVAEALGECGAAGIRVVMITGDHP
                     ATAHAVAEGLGLPHEASGIDVIATGDDLDAADDERLDELVATTNVFARTRPEQKHRLV
                     RALRHQGEVVAMTGDGINDAPALREADIGVAMGQRGTDVAREAAALILLDDNFATIVT
                     AVRDGRRIFDNLTRAFAYLIAFHPPLLLGALVVPLLGEPLLLLPVHLVALELLLHPVV
                     SLVFQADPPDPDTMRRPPRPVGDALRLRALARPYAVGTVLALVLLAVYLVALDVLPED
                     QARALAFASLLASQPVLLLSMRSADRPMWASQRPWTRTLTVVVAVIAVTTVAVVYVPA
                     LAALLHLEPFPAVWWTLAVAVAASTTWSEPFKRRAPGGSPSDPLGPPHRSGGDHLSKA
                     SRSHLEGTALSVAPPPSHSAGQAPLLGAGPSACAASEQPDRQMRAAFRSAHGRLSGHG
                     VHVDPVDHHVQERLGELDVPRREQRQGRQLRRRPPDLGDEDV"
     gene            3713..4360
                     /locus_tag="VLR27_07780"
     CDS             3713..4360
                     /locus_tag="VLR27_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP03383.1"
                     /translation="MEDDDSIGVPLVEGLGFEGYAPTRVRTGAAAVEAVGDTDFVLLD
                     LGLPDLDGAEVCRRLRARSSVPIIVVTAREEEFDRVRLLELGADDYVVKPFGFRELVA
                     RIRAVQRRAGVSSATSRVQSLGPLAIDRHGRRVHVRGEEIDLTPKEFDLLAFLAEVPG
                     VVRTREDIIAQVWDEHWWGPTKTLDVHVASLRRKLAGCDVITTLRGVGYRFDPPE"
     gene            4367..5791
                     /locus_tag="VLR27_07785"
     CDS             4367..5791
                     /locus_tag="VLR27_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP03384.1"
                     /translation="MARRLLITYLTITALALAVVIVPLGSIFAAREKDRLVFDIERDA
                     QTIASLVEDALEAGAEPSIDETLADYGTDGGRIVVVTTAGISVVDSDDTGGAPRDFST
                     RPEMAAALDGQRTSGTRPSETLRTDLMYVAIPVASGGTVHGAVRVTYPTSTLDARVQE
                     TWLRLGLLSIVVLAIVAAVGMVFARGVTRPVQRLQLAAGRLADGDLSVQVPADDGPPE
                     LRDLAETFNSTAARLAQLVESQQRFVADASHQLRTPLTALRLRLETLQPAIAPDARPK
                     LDAAIGETNRLGRLVHSLLVLARSDATTPLCEPVDLTAAIRDRSTAWEPVAADDGTRL
                     VTDCPDGGLWVVAVPEAVEQVLDNLVSNALAAAPPGSTVRIQVEASDHMELHVIDDGP
                     GMTDEERARAFDRFWRPTKEARASEDSGFGLGLAIVHHLATLCGGTARLEPGPGGVGL
                     DASVTFVRAGRESLPDAHLHHSER"
     gene            5863..6228
                     /locus_tag="VLR27_07790"
     CDS             5863..6228
                     /locus_tag="VLR27_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03385.1"
                     /translation="MERRKAITAAAAVSLTLLAGGAGMALSSNIVGTGANDGVGQLSP
                     VSSLDNPPITVYVDDPADPNTAVTVQATDAAPQTTSSASGHNDDDEYEDGDHDEYEDD
                     DEHEDDDDHEEYEGGEDDD"
     gene            6218..6487
                     /locus_tag="VLR27_07795"
     CDS             6218..6487
                     /locus_tag="VLR27_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03386.1"
                     /translation="MTTEAPTGQRHRRRHTATGARIVAGGVSVSVALGLMALMAGVDP
                     DPTVAQGPAAPPPDRVVVVRSSIGAAAAPPVAASNAAPVTSSQSS"
     gene            6490..7410
                     /locus_tag="VLR27_07800"
     CDS             6490..7410
                     /locus_tag="VLR27_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HSP03387.1"
                     /translation="MRTQIAPSTDTRFRAMGTDVHITIVDGPGTLLDLGQRRIAELEG
                     RWSRFRPHSEVSALNHHAGHPVLVSADTYDLVTKAVAAWLATDGRFDPTVGAALIDLG
                     YDRDFGAIRAARPEARSARSAAPTPAGIGLDPAVGAVTLPRGVTFDPGGIGKGLAADL
                     VATVLLESGARGALVNIGGDLRAIGDAPGADGWVITVPEPAGPQEELLRLSIAHGAVA
                     TSSSLRRRWQTTTGEAHHLIDPTTGQPTDSDVHSVTVVAAEAWWAEVLTKYLYVLGPD
                     GLASIDDAHAVVVMADGTRHSTDGIRGALR"
     gene            7407..8111
                     /locus_tag="VLR27_07805"
     CDS             7407..8111
                     /locus_tag="VLR27_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HSP03388.1"
                     /translation="MNPQTWWYVARATGFVAWGLVTTSVITGLLLSTRLARGRPRPAW
                     TLDLHRFLGGTAVVASVLHLAGLVGDSYIHFGAADLFVPFASAWEPGAVALGIVALYL
                     LLAVQGSSMLMRRLPRRLWRIVHLSSFIVFWTATFHLQLAGTDATNPVARWTVNLAMT
                     AVVFLTLVRVLAGRGGSRRGDTRPASGHAARPSSPASSSMSPATGDDHHRAGDASVAH
                     AAEVDGEQVGDECQRV"
     gene            complement(8817..9668)
                     /locus_tag="VLR27_07810"
     CDS             complement(8817..9668)
                     /locus_tag="VLR27_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HSP03389.1"
                     /translation="MDLPRFGADVDAEVLAEALAATGCAVIEQLVPESTMDGIADELA
                     PWMSSTPYGPDDFSGSSTKRTGGLLARSETARELVMHPSVLGAADRVLAHATSYQLHL
                     TQLIAIAPGETGQPIHRDQWAFDFFPFPNGYEVQCNTLWAMTDFTEDNGATRVIPGSN
                     RFEDKQRFTVDDTVPAEMPKGSVLVYTGALYHGGGANRSEAVRCGVNITYNVSWLRQE
                     ENQYLSVPIEVARTFDEDLQRLMGYARGAYALGYVDDLRDPIEVLRAGAGHIGFGDTR
                     ADHLDRT"
     gene            complement(9769..10116)
                     /locus_tag="VLR27_07815"
     CDS             complement(9769..10116)
                     /locus_tag="VLR27_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03390.1"
                     /translation="MAAACAAASQLIGLGVAALSPLVVAVVLGALVANLGRHRSMLAI
                     AVRTAGIIPDRALEAIRAGEVLIFVLALAGLGMGVHAGQLRTLGTRPLLFGLVAWLVI
                     GAAAYGGLRLLTP"
     gene            complement(10142..10738)
                     /locus_tag="VLR27_07820"
     CDS             complement(10142..10738)
                     /locus_tag="VLR27_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP03391.1"
                     /translation="MGPTRTTESDRTPDRDATERALQDAALRLMERNGVLAGLNLREV
                     AAEAGVNRGLVYHYFGSRRELLRASLSRSARRRFTDVAEAGDLPFRERMLAFIQTMVR
                     HREAVRLATLLISDGDESLRTMPLRDLTRQRLAEDVDAGDLDDVDLEAVHVASVSLTY
                     GYVLYRERFAHELGVSVTELDDRFGEIADRMYRGLEPG"
     gene            complement(10747..11598)
                     /locus_tag="VLR27_07825"
     CDS             complement(10747..11598)
                     /locus_tag="VLR27_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HSP03392.1"
                     /translation="MHGTKTDVQVVPTGGPLGAEVRGVDLAGDVDGETVFAITEAFRR
                     HHVLLFRGQDITSERQLEIAEWFGPRYVPPRDIPVLGDETQPPVVAVSNDPGGVLGDG
                     ELAAHSDLQYMPVPLLGSILVAEVIPPAGGDTSWSNLHLAYDELDTDLRSRIEGRRAW
                     AFNPYAGSRAIRSLAGPNQRYVEEDVQPFFHPLVRIHPDTGRRSLYVSYLTAGIEGMD
                     EVESRELLRALVAHVDEDRFYWTHQWRPGDVVLWDNRCTNHKRTSIDPSDRRVLHRVQ
                     IAGTRPF"
     gene            11758..12531
                     /locus_tag="VLR27_07830"
     CDS             11758..12531
                     /locus_tag="VLR27_07830"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HSP03393.1"
                     /translation="MSITSPALTVERLTGSTGARIRDVDLNALDDGAFVAIRQALLEH
                     GVVHLPGQEVTPEAHKAFGERFGDLHTHPAAPGVDGHPEILLLENRGKSHTITEVWHS
                     DVTCETKPPAISILAARALPDAGGDTMWANQYLAYDRLSDGMKAMLGDMRAVHKAFGL
                     EAVHPVIRTHPETGRKALFVNGGFTSHFENMTKAESKPLLEYLVAFASTPDLTYRHSW
                     SAGDVVLWDNRGVMHFAVHDYEDQHRLMHRVTVAGDEPR"
     gene            12579..13424
                     /locus_tag="VLR27_07835"
     CDS             12579..13424
                     /locus_tag="VLR27_07835"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HSP03394.1"
                     /translation="MTIADREANAITVTPINPTLGAEVHGVDLADLDDATWARIEQAW
                     ADHAVLFFRDQDLPPEAHEALGRRIGELHVHPAAPTVEGHPNVMTIHADENSKVVAGN
                     GWHTDVSCDERPPAATILHLPVIPAVGGDTLFISTEAAYDALSPAMQDFLDDKVALHE
                     SAHVYKGRYGSKESASRDGQFPSADHPVIRTHPVTGRKAIYVNRSFTTRIKGLSLNES
                     RAILDLLFDHIEHRPEFQVRFTWTPGAVAMWDNRCTQHYAAWDYFPNVRSGRRVSVVG
                     ERPFR"
     gene            13421..15208
                     /locus_tag="VLR27_07840"
     CDS             13421..15208
                     /locus_tag="VLR27_07840"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSP03395.1"
                     /translation="MTTVDKDALKQKYAIERAKRLRPDGNDQYLEVKGQLAHYLEDPY
                     TERVEREPKTDHVTVTFIGGGFAGLVTGARLKEAGVEDVRLIDKGGDFGGTWYWNRYP
                     GAQCDTASLVYMPLLEETGHMPTEKYAHAPEILEHCQRIGKHFGLYDDALFHTEVTDL
                     EWDDRRSHWIVRTTRGDEFTSTHVAMGTGPLHVPKLPGIDGIERFEGHSFHTSRWDYD
                     YTGGDPTGAPMDRLADKRVAIIGTGATSVQCIPHLARACEELYVFQRTPSSVDERNNE
                     PIDPEWFAQIATPGWQERWLKNFTDNQTGGMVDEDLVMDGWTELARRIRSRIMSLPLP
                     DRTPEKMMEAFEDSDFEKMEEIRQRVERIVDDPETAEDLKAWYRQLCKRPCFHDEYLQ
                     AYNEPGTHLVDTDGKGVERITPRGVVANGVEYAIDCIIYASGFEVGTDHTRRRGFDIR
                     GRDGLALSDYWADGMRTLHGIHVHGFPNLFIVQPTQGANLISNVPHNLTESGKTIALV
                     IAHALANGHEEVEVTKDAEDTWIDLLLSGGGRMLGSPDCTPGYYNNEGQDPGPAAAFF
                     VGYPQGASAFFDYIDRWRSTGRFDGLEFR"
     gene            15235..16485
                     /locus_tag="VLR27_07845"
     CDS             15235..16485
                     /locus_tag="VLR27_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HSP03396.1"
                     /translation="MQPTATMTPDEALLHLFSSPEALSDPAPLYRFLREEAPRHRSSV
                     GSIVLTRFDDCHEVLRDNRFGKGQNPDGGGMLPMADPEAVKMRAELAARRDRAPSMLF
                     LNPPDHTRQRQLVARTFTPRRVEQMRASIAELARRCIDDLAEAGSGDLLDLVAFPLPV
                     AVIGALVGVPEADWTGFRSLITTAAAGIEVAASADDLRAAADANQEVGRYFMDLIGHR
                     RRHPEDDLLSDLLAVEEAGDRLSEAEVISVASLLFAAGFETTTNLIGNGMGALLRHPD
                     QMAQLWADPALLPSAVEEILRWDSPVQFDARTALQPADLFGEHVEEGGRIITLLGAAN
                     RDPGHFTDPDRFDVTRDEGPPMSFASGIHYCLGANLARAEGQEVFRALRDRFSAIELN
                     GELRQRPRMTLRGYESVPVTVTPR"
BASE COUNT         2703 a         6048 c         5650 g         2365 t
ORIGIN      
        1 ctggatgatc tgccggtcgg atggtcgtcc aagcctcgag acgacgagga agaggacgac
       61 gacgagtacg accaggaact cgccgactgc atggacatgc cggtcgagga gttcgcggac
      121 tccgggaacc cgaaggccga gtcggagacc ttcgtcgccg aggacgactc cgaggtcgaa
      181 gccgaagtcg tgatcgcacc gaccgtggac gaagcgaccg aggacttcga gcgagcaacc
      241 tccccggagt tcctcgactg catgcgtgag gtgttgccgg cgctgatgga acaggctgcg
      301 gaggacgaag gaggcgagtt cacgatcgcc gacgcttcga tcggaccgct gcgcatcgag
      361 gactccgggg accggtcggc ggcgttccgc ctgacgatct cggtcgaggt cgacgccttc
      421 agcgtcgact tctactcaga catcctcgtc gcccaggtcg ggcggtcgac ggcgcagctg
      481 tcgaccttgt cgttgttctc gccgagggac gtcgagttct cccagtcgct cctggacgtg
      541 atggtcgacc ggatcgacgt ggacgccgtg tcctgacagc cgcccatgcg cggatcggaa
      601 cgccgcccgc atctgccggt ccggctgttc gctggctgca caagcagaag gccccgctcc
      661 gagaagtggg gcctgacctg cggagtgcga tggtggcggg gcaacggaga gtgcggtgcc
      721 ttcgaggtgg ctccgggatg ccttggacag gtggtcccca ccgcttcgat gaggcggacc
      781 gagcgggtcg gaggggctac cgccgggtgc tcgccgcttg aacggctcgg accaggtggt
      841 gctggccgcc acggccaccg ccagcgtcca ccacacggcg gggaacggct cgaggtggag
      901 cagcgcagcg agggctggga cgtagaccac cgccacggtg gtgacggcga tcacggcgac
      961 gacgacggtg agcgtccggg tccacggtcg ttggctggcc cacatcggtc ggtcggcgct
     1021 gcgcatgctg agcagcagca ccggctgact cgccagaagc gatgcgaagg cgagcgctcg
     1081 ggcttggtcc tcggggagca cgtccagtgc gacgaggtag acggcgagga gcacgagggc
     1141 gagcacggtc cccacggcgt agggccgagc cagggcgcgg aggcgcagag cgtcgccgac
     1201 tggtcggggt gggcgtcgca tcgtgtccgg gtcgggtggg tcggcctgga acacgaggga
     1261 cacgaccggg tgcaggagca gctcgagcgc gacgaggtgg acgggcagga gcagcagcgg
     1321 ctcgcccagc agcgggacga cgagcgcccc gagcagcagc ggcgggtgga acgcgatcag
     1381 gtaggcgaat gcccgcgtga ggttgtcgaa gatgcggcga ccgtcgcgca cggcggtgac
     1441 gatggtggcg aagttgtcat cgagcaggat gagcgctgct gcctcccgcg cgacgtcggt
     1501 gccgcgctgc cccatggcga cgccgatgtc ggcttcccgc agggcgggcg cgtcgttgat
     1561 gccatcgccg gtcatggcga cgacctcacc ctggtgtcgg agtgcgcgga ccaggcggtg
     1621 cttctgctcg gggcgggtgc gggcgaagac gttggtggtg gcgacgagct cgtcgagccg
     1681 ctcgtcgtct gccgcgtcca ggtcatcccc ggtggcgatc acatcgatcc cgctggcctc
     1741 gtgcggcagg cccagcccct cggcgaccgc atgcgcggtg gcggggtggt cgccggtgat
     1801 catgacgact cggatgccgg cagcgccgca ttcgccgagg gcttcggcga cgccatcacg
     1861 gagcggatcg ctgaaggcga cgagaccgag gagcctcagg tgcgcctcgt catcggatcg
     1921 gctcgtcgcc gtcacattgg ctctgcccgc ggcgacagcg acgacgcgca tgccactttc
     1981 actgaacgcc gtgttggcct gcgctgctgc tgcgcgctgc gcgccgtctg cgtgggatcg
     2041 agcgaggatg ccttcgatgg cacctttcgc ggcgacccac atggtgtcgt cctccccgga
     2101 tcgccagacg tgggtgaggt acttgtcggt cgggtcgaac ggccagtccc tcgccagccc
     2161 gccgccgtgc aatcgggcga cgtcgacgtc gtggcaacgg gcgtggtcga cgatggcgac
     2221 gtcgaggggg tcgtaggggt ccggttcgca ggcgaggacg gcagcctcga tgaggtcgac
     2281 ctctgcttgg ctgggggacc cgccatcgga gcccgcgccg tcggtggtcc acagtccggc
     2341 cacggcgagc cgaccgtggg tgagtgtgcc cgtcttgtcc gtgcagatga ccgtcgtgga
     2401 tccgagggtc tcgacgctgg gcagtcgccg gacgagggcg cggtcctggg cgagtcgcca
     2461 cgcgccgagc gccaggtaca gcgtgtagat catcgagaac tcttcgggga tggccgcgat
     2521 cgcgaggctg acccctgcga tcacggcatc gccccacccg tcaccgtgca ggagcgttgc
     2581 agcgacgacc gcgatgcaga acacgccggc gacgacggtg aggccgcgca ccaagcggac
     2641 cagtccccgc tgcagcggag tgaggggcga tcgaccggcg gccacgagcg caccgatctg
     2701 cccgtaccgg gtggcgcgtc ccgtgccggt gacgcgcgcc agcgctcgcc ccgacaggac
     2761 ggtggtcccc gcccagatca tcgcctcgtc gccgacggcc tcgacctgct tgctggcggg
     2821 cagcgactcg ccggtgagtg acgactcgtc gacgaggagc tgggtcagct cggtcaccgc
     2881 cgcgtcagcg ggtaccacgt caccctcgag gacgcgcacc agatctccga cgacgagcga
     2941 gctggcggga acggtctgat cgacaccgtc tcgaacgacg gcggcggttc gcgctgcgag
     3001 gcgccggagc tggtcgaggg ttcgctcggc ccgggactcg agcagccaac cgaccgcggc
     3061 gatcggcccg agcgccacga gcgccacgag cgcatccgtg gtctccccga tcagcaggta
     3121 ggtcggcgcg gcgacgagca agagagcgac catcggatcc gcaaacggcc cgaggaggcg
     3181 cttgagccgc tggccgcggt gcacgggcac gatgtcgttc ggcccgtcgc gggccagccg
     3241 ctccgccgcc tccccgctcg tcagcccacg ggcggacaca ccgacgatgg ggccatcgac
     3301 ctccacgacg gactccttcc agcgacgagc gcgcccatcg ctccttcgtc tcgacgcctg
     3361 gccatcgtgc tgctcacgcc gggctggcga cagaggcgaa cgtcccccgg gtggagatgc
     3421 cgatgagcac gctgccgagc gtcgttccga tctgggtcac gtggcaaacg cacgaactcc
     3481 catgtcacga cggcaccaga gcaggtctag gaccccggaa aggcggcgac gggtgcgaac
     3541 cgcaggcgtc cgagttgttc ggaaggtggt cgacaatcag gaccttcggc ccgggtggta
     3601 ccgctggtcg tcagtgatcg tggagccgat gggtgacgtc gcgagccccg acaggtcggt
     3661 cctgccgaca tcgactgacc ccgcgaccac gggatcgagt cgcgtcctgg tcgtggagga
     3721 cgatgactcg atcggcgtgc ctctggtcga aggactcggg ttcgaggggt acgcacccac
     3781 ccgggtccgc accggtgccg ctgcggtgga ggcggtaggc gacacggact tcgtgctgct
     3841 cgacctgggg ttgcctgacc tggacggcgc tgaggtgtgc cgacgactgc gggctcgctc
     3901 ctcggtgccg atcatcgtgg tcaccgcacg cgaggaggag ttcgaccggg tccggctgct
     3961 cgagctcggc gccgacgact acgtggtgaa gccgttcggg ttccgggagc tggtcgcgcg
     4021 gatccgggcg gtgcaacgcc gggccggcgt gtccagcgcg accagccgtg tgcagagcct
     4081 cggaccgctc gcgatcgacc gtcacgggcg ccgcgtgcac gtccgcggcg aggagatcga
     4141 tctcacgccc aaggagttcg atctgctcgc gttcctggcg gaggtaccgg gtgttgtccg
     4201 aacccgtgag gacatcatcg cccaggtgtg ggacgagcac tggtgggggc cgacgaagac
     4261 cttggatgtc cacgtggcgt cgctgcgtcg caagctcgcc ggctgcgacg tcatcaccac
     4321 gcttcgcggc gtcggctatc gcttcgaccc gcccgagtag acggcgatgg cacggcggct
     4381 cctgatcacg tacctcacca tcaccgcgct cgccctcgca gtggtcatcg tccccctcgg
     4441 aagcatcttc gccgctcgtg agaaggaccg cctggtcttc gacatcgagc gcgatgccca
     4501 gaccatcgcc tcgctcgtgg aggacgcgct cgaggccggg gccgagccga gcatcgacga
     4561 gaccctcgcc gactacggga ccgacggcgg ccgcatcgtc gtggtgacca ccgctggcat
     4621 ctccgtcgtc gactccgacg acaccggggg tgcgccaagg gacttctcca cccggcccga
     4681 gatggcggcg gcgctcgatg gccaacgcac ctcgggcacc cgaccatcgg agacgctccg
     4741 caccgatctg atgtacgtcg ccatccccgt cgcgtccggg gggacggtcc acggtgcggt
     4801 gcgggtcacc tacccgacct ccacactcga cgcccgtgtc caggagacgt ggttgcggct
     4861 gggtctgctc tcgatcgtcg tgctcgccat cgtcgcggcg gtcgggatgg tcttcgcccg
     4921 gggtgtcacc cgaccggtgc agcgcctcca gctggccgcc ggacgcctcg ccgacggcga
     4981 cctgtcggtg caggtgcccg ccgatgacgg accgccggag ctgcgggacc tcgccgagac
     5041 gttcaactcg accgcagctc ggctcgcgca gctcgtcgag tcccagcaac ggttcgtcgc
     5101 cgacgcgtcc catcagctcc ggacgcccct caccgcgctc cggcttcggc tcgagacgct
     5161 ccagcccgcg atcgcgcccg atgcgcgccc caagctcgat gccgcgatcg gggagaccaa
     5221 ccggctcggt cgcctcgtcc actcgctgct cgtactcgcc cgcagcgacg ccaccacccc
     5281 gctgtgcgag ccggtcgacc tcactgccgc gatccgcgac cggtcgacgg cgtgggagcc
     5341 ggtggccgcc gacgacggca cgcggctcgt caccgactgc ccggacggcg gcctgtgggt
     5401 cgtcgcagtc cccgaggccg tggagcaggt gctggacaac ctggtctcca acgcgctcgc
     5461 cgccgcaccg ccgggctcca ccgtgcgcat ccaggtcgag gcatccgacc acatggagct
     5521 gcacgtgatc gatgacgggc cgggcatgac cgacgaggaa cgagcccggg ccttcgaccg
     5581 gttctggcgg ccgacgaagg aagcgagagc cagcgaggac agcggcttcg gcctcggctt
     5641 ggccatcgtg caccacctcg ccaccctctg cggcgggacc gcgcgactcg aacccggacc
     5701 cggaggtgtc gggctcgacg cctccgtcac cttcgtccgg gcggggcgag aatccttacc
     5761 ggacgctcac ctccaccaca gcgagcgctg accagcacct ccgtagcgtc gactccagaa
     5821 ccaccgagcc cccaccgggg ccaacgatca ggagctcacg ccatggaacg tcgcaaggcc
     5881 atcaccgccg cagccgccgt ctcgctcacc ctgctcgccg gcggagccgg catggcgctg
     5941 agctccaaca tcgtcggcac gggagccaac gacggcgtcg gtcagctgag cccggtcagc
     6001 tcgctcgaca accctccgat caccgtctac gtcgatgacc cggccgatcc gaacaccgca
     6061 gtgacggtgc aggcgaccga cgcagcaccg cagaccacct cgtcggccag cggccacaac
     6121 gatgacgacg agtacgaaga tggcgaccac gacgagtacg aggacgacga cgagcacgaa
     6181 gacgacgacg accacgagga gtacgagggc ggcgaggatg acgactgagg ccccgaccgg
     6241 gcagcgccat cgccggcggc acaccgccac cggtgcccgg atcgtcgccg ggggcgtcag
     6301 cgtcagcgtc gcactcggcc tgatggcgct gatggccggt gtcgaccccg atccgaccgt
     6361 ggcgcaaggc cccgccgcac ccccgcccga ccgggtcgtc gtcgtccggt cgagcatcgg
     6421 cgccgccgct gcgccgccgg tcgcggcatc gaatgccgca cccgtcacgt cgagccagtc
     6481 gagctgacca tgcgaaccca gatcgcgccc agcaccgaca cgcggttccg ggcgatgggg
     6541 accgacgtcc acatcaccat cgtggacgga cctggcaccc tgctcgatct cggtcagcgc
     6601 aggatcgccg agctggaagg tcgatggagt cgcttccgcc cccacagcga ggtgagcgcc
     6661 ctcaaccacc acgccggcca ccccgtcctc gtctccgccg acacctacga cctcgtcacc
     6721 aaggccgtgg ccgcgtggct ggccaccgac ggacgcttcg acccgaccgt cggcgcagcc
     6781 ctcatcgatc tcggctacga ccgggacttc ggcgccatcc gcgccgcccg gcccgaggcg
     6841 cggtccgccc gctcggccgc ccccacaccc gccggcatcg gcctcgaccc cgccgtcggc
     6901 gccgtcaccc tcccccgagg cgtcaccttc gatcccggcg gcatcggcaa gggcctggcc
     6961 gccgacctcg tggccaccgt gctcctcgag tccggcgcac gaggcgcgct cgtgaacatc
     7021 ggcggcgacc tccgggccat cggcgatgca cccggtgcgg acgggtgggt catcaccgtc
     7081 cccgagcccg ccggccctca ggaggagctg ctccgactct cgatcgccca cggagcagtc
     7141 gccaccagct ccagcctccg acgccgctgg cagaccacca caggcgaagc ccaccacctc
     7201 atcgacccca ccaccggaca accgaccgac tccgacgtgc acagcgtcac cgtcgtcgcc
     7261 gcggaggcgt ggtgggccga ggtgctcacc aagtacctgt acgtgctcgg cccggacgga
     7321 ctggcgtcga tcgacgacgc ccacgccgtc gtcgtcatgg ccgacggcac acgacactcg
     7381 accgacggca tccgaggggc gttgcgatga acccgcagac ctggtggtac gtcgcccggg
     7441 cgacggggtt cgtcgcgtgg gggctggtca ccacctcggt catcaccggc ctgctgctca
     7501 gcacccggct ggccagaggc agaccacggc cggcatggac cctcgacctg caccgcttcc
     7561 tcggcgggac cgccgtcgtt gcctccgtcc tgcaccttgc tggccttgtg ggagacagct
     7621 acatccactt cggggcggct gacctgttcg tccccttcgc ttccgcgtgg gagcccggcg
     7681 ccgtcgcgct cgggatcgtc gccctctacc tgctcctcgc cgtgcagggc tcgtccatgc
     7741 tgatgcgccg cctcccgcga cggctctggc gcatcgtcca cctgtcgagc ttcatcgtct
     7801 tctggaccgc caccttccac ctgcagctcg ccggcaccga tgccaccaac ccagtcgccc
     7861 gttggaccgt caacctggcc atgaccgccg tcgtcttcct caccctcgtg cgcgtcctcg
     7921 ccggccgagg gggctcacgg cgaggcgaca cccgaccggc gtccggtcac gcggctcgcc
     7981 cttcgtcgcc ggcgagctcc tcgatgtcgc ctgcgaccgg cgacgaccac caccgggcag
     8041 gtgatgcttc cgtcgctcat gccgctgagg tcgacggaga acaggtcggc gacgaatgcc
     8101 aacgggtatg accgcagccc gagcggatcg gacaggaact gctccttggt ccatggctcc
     8161 cgacagacgc gaccgatgtc caggtagagc cagaacggga ccgggaggcg cggtgcgatc
     8221 gttcccccga gacggagcgc tcgctggagc ggtcggtgca ggtggcggag ccacgcgggc
     8281 aaccgggtca ccagcagcga atcccgcagg accgggtcca gcacgttgtg tgccaccacg
     8341 cccgcgacgg gcacgccgcg ggccgctgct gccatggcca ggatccctcc ctggctcgag
     8401 cccagcagca ccggcaggca cccgaatcga tcggtcgccc agacaacagc ctccgcaacg
     8461 tcctcgacca ggcgctccag gcgcagcacg cgctgctggc gagggctctt gccgtgcccc
     8521 tcgaagtgca caccgacgac ggtcgtgccc cgggatgcca gtgcggcaca gaacgcctcg
     8581 tggaacagcg ggtgggtcat cgtgcccggt aagaacacca cgaccgggcg gccctgtacc
     8641 ccgtgccaga cactgagaac caccggagcg gcgggtgaga aacgtggggc atgcgctcat
     8701 gcggcggagt gtgccgcctc ggccagtact cgcactcgaa tcgctgacgg cgaccgatcc
     8761 acgcccggca gtcgctccaa gccgccgtgc cgggcctccg gtcccatgaa gggtgctcac
     8821 gtccggtcga ggtgatccgc tctcgtgtct ccgaagccga tgtggccggc gccagcgcgg
     8881 agcacttcga tcgggtcccg caggtcgtcc acgtacccca gtgcatacgc gccgcgggcg
     8941 tagcccatga gccgttggag atcctcgtca aacgtgcgcg ccacctcgat tgggaccgac
     9001 aggtactggt tctcctcctg gcgcagccac gacacgttgt aggtgatgtt gaccccacag
     9061 cgcaccgcct cggaccggtt cgcaccgccg ccgtggtaga gcgcgccggt gtagacgagg
     9121 acggaaccct tcggcatctc ggccgggacg gtgtcgtcca ccgtgaaccg ctgcttgtcc
     9181 tcgaagcggt tgctgcccgg gatcacccgg gtggcgccgt tgtcctcggt gaagtcggtc
     9241 atcgcccaga gcgtgttgca ctggacctcg tagccgttcg ggaaggggaa gaagtcgaag
     9301 gcccactggt cgcggtggat gggctggccc gtctcaccag gagcgatcgc gatcagctgg
     9361 gtcaggtgca gctggtacga cgtggcgtgg gcgaggacac ggtcggctgc gccgaggacc
     9421 gaggggtgca tcaccagctc gcgggccgtc tccgacctcg ccagcaggcc gccggtgcgc
     9481 ttggtcgacg aaccggagaa gtcgtcgggt ccgtagggcg tcgacgacat ccacggcgcc
     9541 agctcgtcgg cgatgccatc catcgtcgac tcgggcacga gctgctcgat gaccgcgcac
     9601 cccgttgcgg cgagggcctc agcgagcacc tcggcgtcca cgtcggcgcc gaaccttggg
     9661 aggtccatca cgtctcctgt cgatctgctg ctgcgccgac gctacgtgct caccgccacg
     9721 acatggcgaa catcgagtcg taggcgtagc cccatggcgg tccggacctc acggtgtcag
     9781 cagccgtagg cccccgtagg cggcggcgcc gatcacgagc caggcgacca gtccgaagag
     9841 cagggggcgg gtgccgagcg tgcggagctg gccggcgtgg acgcccatgc cgagtccggc
     9901 gagggcgagg acgaagatca ggacctcacc ggcccggatg gcctcgagcg cccggtccgg
     9961 gatgatcccg gcggttcgca cggcgatggc gagcatcgag cggtgccgac cgaggttcgc
    10021 gacgagagcg ccgaggacca cggcgacgac cagaggtgac agggcggcga cgccgagacc
    10081 gatcagctga cttgcggcgg cgcaggcagc cgccaccgcg acgccgggcc gacaccaccg
    10141 ctcaaccggg ctcgagtccc cggtacatgc ggtcggcgat ctcaccgaag cggtcgtcga
    10201 gctcggtgac ggagacgccg agctcgtggg cgaagcgctc gcggtacagg acgtagccgt
    10261 aggtgagcga cacggaggca acgtgcacgg cctcgaggtc cacgtcgtcg aggtccccgg
    10321 catcgacgtc ctccgcgagc cgctggcggg tgaggtcccg caggggcatg gtgcgcaacg
    10381 actcgtcgcc gtcgctgatc agcaaggtgg cgagccggac ggcctcgcga tggcggacca
    10441 tcgtctggat gaaggcgagc atccgttccc ggaaggggag gtcgccggcc tcggccacgt
    10501 cggtgaagcg cctgcgggcc gagcgggaca acgacgcacg cagcagctca cgacgggatc
    10561 cgaagtagtg gtacacgagc ccgcggttga cgccggcctc ggcggcgacc tctcgcaggt
    10621 tcaggccggc gagcaccccg ttgcgctcca tcagccgcag ggccgcgtcc tgcagggccc
    10681 gttcggtcgc gtcgcggtcc ggtgtccggt ccgattcggt ggttctcgtc ggtcccacgt
    10741 cgttgctcag aacgggcggg tgccggcgat ctgcacccgg tgcaggaccc gccggtcgga
    10801 tgggtcgatg gaggtgcgct tgtggttggt gcagcggttg tcccagagca cgacgtcacc
    10861 ggggcgccat tggtgcgtcc agtagaagcg gtcctcgtcg acgtgggcca cgagcgctcg
    10921 cagcagctcg cgggactcga cctcgtccat gccctcgatg ccggcggtga ggtaggagac
    10981 gtagagcgag cgccgtcccg tgtcggggtg gatccgcacc aacgggtgga agaacggctg
    11041 cacgtcttcc tcgacgtagc gctggttcgg gccggccagc gagcggatcg cccgcgaccc
    11101 tgcgtacggg ttgaacgccc acgcccggcg cccctcgatg cgagagcgaa ggtcggtgtc
    11161 gagctcgtcg taagccaggt gcaggttgga ccacgacgtg tccccaccgg cgggtgggat
    11221 gacctccgcg accaggatcg aaccgagcag cgggaccggc atgtactgca ggtccgagtg
    11281 cgcagccagc tccccgtcgc ccagcacacc gccgggatcg ttcgagacgg cgaccaccgg
    11341 cggctgggtc tcgtcgccga gcaccgggat gtctcgcggc gggacgtagc gagggccgaa
    11401 ccactcggcg atctcgagct gacgctcgct ggtgatgtcc tggccccgga acaacagcac
    11461 gtgatggcga cggaacgctt cggtgatggc gaacaccgtc tcgccgtcca cgtcaccggc
    11521 cagatccacg ccgcggacct cagcgcccag tgggccaccg gtcgggacta cctgcacgtc
    11581 cgtcttcgtt ccatgcatcg gtgtggtgtc ggtcacagag ctccctcggt cggatcagcg
    11641 tccggcggac gagccggccc ctggtgcgat gatcctcggc tcgttagcat tctgtcaaca
    11701 gcaccgctgc ggcaaagacg ccggcgagtc tccggcgcgg tctcttgggg gatgtccatg
    11761 tcgatcacca gccctgcgct caccgtcgag cggctcaccg gttccaccgg cgcccgcatt
    11821 cgagacgtcg atctcaacgc cctcgacgat ggagcattcg tcgcgatccg acaggccctg
    11881 ctcgaacacg gggtcgtcca cctcccgggc caggaggtga cccccgaggc gcacaaggcc
    11941 ttcggcgagc gcttcggcga tctccacacc cacccggccg ccccgggtgt cgacggccat
    12001 cccgagatcc tgctgctcga gaaccgcggc aagtcccaca ccatcaccga ggtgtggcac
    12061 tccgacgtga cctgcgagac gaagccaccg gcgatctcga tcctcgcggc ccgagcgctg
    12121 cccgatgccg gcggcgacac gatgtgggcg aaccagtacc tggcctacga ccggctctcc
    12181 gacggcatga aggcgatgct cggggacatg cgggccgtcc acaaggcgtt cggcctcgaa
    12241 gccgtccacc cggtgatccg cacccacccc gagaccgggc gcaaggcgct gttcgtcaac
    12301 ggcgggttca cctctcactt cgagaacatg accaaggccg agtccaagcc gctcctcgag
    12361 tacctcgtgg ccttcgccag caccccggat ctcacctacc ggcacagctg gagcgccggt
    12421 gacgtcgtgc tgtgggacaa ccggggtgtc atgcacttcg ccgtccacga ctacgaggac
    12481 cagcaccggc tcatgcaccg cgtcaccgtc gccggcgacg aaccccgctg accaccaccg
    12541 accgacacca gagcccgacc acccgtgagg aacccgacat gaccatcgcc gaccgcgagg
    12601 cgaacgcgat caccgtcacc ccgatcaatc cgaccctcgg cgccgaggtg cacggcgtcg
    12661 acctggccga cctcgacgac gccacctggg cccggatcga acaggcctgg gccgaccacg
    12721 ccgtgctgtt cttccgcgac caggacctcc cacccgaggc ccacgaagca ctcggccgcc
    12781 gcatcggcga gctccacgtc cacccggccg cacccaccgt ggaaggacat cccaacgtga
    12841 tgaccatcca cgcagacgag aactccaagg tcgtggccgg caacggctgg cacaccgacg
    12901 tcagctgcga cgaacgccca ccggcagcga ccatcctgca cctccccgtc atccccgccg
    12961 tggggggcga cacgctcttc atctccaccg aagccgccta cgacgccctc tcccccgcca
    13021 tgcaggactt cctcgacgac aaggtggccc tgcacgaatc ggcccacgtg tacaagggcc
    13081 gctacgggtc caaggaatcc gcatcacgcg acggccagtt cccctccgcc gaccacccgg
    13141 tcatccgcac ccacccggtc accggccgca aggccatcta cgtcaaccgc tcgttcacga
    13201 cccggatcaa gggcctttcc ctcaacgaga gccgggcgat cctcgacctg ttgttcgacc
    13261 acatcgagca ccgccccgag ttccaggtcc ggttcacctg gacccccggg gccgtggcca
    13321 tgtgggacaa ccgctgcacc cagcactacg ccgcctggga ctacttcccc aacgtccgaa
    13381 gcggccgccg ggtcagcgtc gtcggggagc ggccgttccg atgacgaccg tggacaagga
    13441 cgccctgaag cagaagtacg ccatcgagcg ggcgaagcgc ctccggcccg acggcaacga
    13501 ccagtacctg gaggtgaagg ggcagctcgc ccactacctc gaggacccct acaccgagcg
    13561 ggtcgagcgc gaaccgaaga cggaccacgt gaccgtcacc ttcatcggcg gtggcttcgc
    13621 cgggctcgtc accggtgccc gcttgaagga ggccggcgtc gaggacgtgc gcctcatcga
    13681 caagggcggc gacttcggcg gcacctggta ctggaaccgc tacccgggcg cgcagtgcga
    13741 cacggcgtcg ctcgtgtaca tgccgctgct cgaggagacc gggcacatgc ccaccgagaa
    13801 gtacgcgcac gcacccgaga tcctggagca ctgccagcgg atcgggaagc atttcgggct
    13861 ctacgacgac gcgctgttcc acaccgaggt caccgacctc gagtgggacg accgtcgctc
    13921 gcactggatc gtgcgcacca cccgcggtga cgagttcacg tccacccacg tggccatggg
    13981 caccggaccc ctccacgtcc ccaagttgcc gggcatcgac ggcatcgagc gcttcgaggg
    14041 ccacagcttc cacaccagcc gatgggacta cgactacacg ggcggcgacc ccaccggggc
    14101 acccatggac cgtctggccg acaagcgggt ggccatcatc ggcaccgggg cgacctccgt
    14161 ccagtgcatc ccccacctcg cccgggcgtg cgaggagctc tacgtcttcc agcgcacccc
    14221 gtcctcggtc gacgagcgca acaacgagcc catcgacccg gagtggttcg cgcagatcgc
    14281 cacgccgggc tggcaggaac gctggctgaa gaacttcacc gacaaccaga ccggcgggat
    14341 ggtcgacgag gacctggtca tggacggctg gaccgagctg gccaggcgca tccgcagtcg
    14401 catcatgtcg ctcccgttgc cggaccggac gcccgagaag atgatggagg ccttcgagga
    14461 ctccgacttc gagaagatgg aggagatccg ccagcgggtc gagcgcatcg tcgacgatcc
    14521 cgaaaccgcc gaggacctca aggcctggta ccgccagctg tgcaagcgcc cgtgcttcca
    14581 cgacgagtac ctccaggcct acaacgagcc cggcacgcac ctcgtcgaca ccgacggcaa
    14641 gggcgtcgag cgcatcaccc cccgcggcgt ggtcgcgaac ggcgtcgagt acgccatcga
    14701 ctgcatcatc tacgcctccg gcttcgaggt cggcaccgac cacacgaggc gccgcggctt
    14761 cgacatccga ggtcgggacg gtctcgcact ctccgactac tgggccgacg gcatgcggac
    14821 cctgcacggc atccacgtcc acgggttccc gaacctgttc atcgtccaac cgacccaggg
    14881 cgccaacctc atctcgaacg tgccccacaa cctcaccgag tccggcaaga cgatcgccct
    14941 cgtcatcgcc cacgcgctgg cgaacggcca cgaggaagtc gaggtcacca aggacgccga
    15001 ggacacctgg atcgacctcc tgctgtccgg cggcggacgg atgctcggct ccccggactg
    15061 cacccccggc tactacaaca acgagggtca ggaccccggc cccgcggcag cgttcttcgt
    15121 cggatacccg cagggggcgt cggcgttctt cgactacatc gaccgttggc ggagcacggg
    15181 acgcttcgac ggcctcgagt tccgctgaac acaccaacac aggagcgaac gactgtgcag
    15241 ccgaccgcca ccatgacgcc cgacgaggcc ctcctccacc tgttctccag ccccgaggcc
    15301 ctctccgatc cagccccgct gtaccggttc ctgcgcgagg aggcgccccg gcaccggtcc
    15361 tccgtcggct cgatcgtcct cacccgattc gacgactgcc acgaggtgct gcgggacaac
    15421 cggttcggca agggccagaa ccccgacggc ggcgggatgc tccccatggc ggaccccgag
    15481 gccgtcaaga tgcgggccga gctcgcggcc cgccgcgacc gcgctccgtc gatgctgttc
    15541 ctgaacccgc cggaccacac ccggcagcgg cagctcgtgg cccgcacctt cacacctcgg
    15601 cgggtggagc agatgcgagc ctccatcgcc gagctcgccc gccgctgcat cgacgacctc
    15661 gccgaagctg gaagcggcga tctcctcgac ctggtcgcct ttcccctgcc tgtcgcggtc
    15721 atcggtgcgc tcgtcggcgt gcccgaagcc gactggaccg ggttccggtc cttgatcacc
    15781 acagctgcag ccggcatcga ggtcgctgcg tcggccgatg acctgcgggc ggcggccgac
    15841 gccaaccagg aggtcgggcg ctacttcatg gacctgatcg gccaccgtcg gcgccatccc
    15901 gaggacgacc tgctctccga cctcctcgct gtggaagagg ccggtgatcg gctcagcgaa
    15961 gccgaggtca tctcggtcgc gtcgctgctg ttcgccgccg gcttcgagac gaccaccaac
    16021 ctgatcggca acggcatggg tgcgctgttg cgccacccgg accagatggc gcagctctgg
    16081 gccgaccccg ccctgctccc cagcgcagtg gaggagatcc tgcggtggga ctcgccggtc
    16141 cagttcgatg cccggaccgc cctgcaaccg gccgacctct tcggtgagca cgtcgaagaa
    16201 ggcggacgaa tcatcaccct gctcggggcc gccaaccggg acccaggcca cttcaccgac
    16261 cccgaccgct tcgacgtcac ccgtgacgag gggccaccga tgagcttcgc atcaggcatc
    16321 cactactgct tgggtgcaaa cctcgcccga gccgagggcc aagaggtctt ccgcgcactg
    16381 cgcgaccggt tctccgccat cgagttgaac ggggaactac gtcaacgccc ccgcatgacc
    16441 ttgcgtgggt acgagtcggt tcccgtgact gtcacgcccc gctgacaagg ggtgaccgca
    16501 ggagcgcctg tcaccctccg tgggcaccag gccgccagag cgcacccagg tgcgcagagc
    16561 acgagcagtc ccgtttggct gccatccgtc gccaggcctc acagcatgac gaggacgccg
    16621 ctcaccgcga tcaggctgta cgcgtatcgg gcgaacctct ccggtgggac tcggcggtgg
    16681 agagcctggc cgaggagtac ggcaagcagg acccccggga gactgacggc caacagctgg
    16741 aagacctgcc atgtccacag cccgcc
//
LOCUS       DATISU010000191        29089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_288777, whole genome shotgun sequence.
ACCESSION   DATISU010000191 DATISU010000000
VERSION     DATISU010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 29089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29089
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288777"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VLR27_07850"
     CDS             <1..341
                     /locus_tag="VLR27_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03397.1"
                     /translation="DAKPGEAKGERKADKPKGDKPKADEPKGDEPKAEEPKRDEPTAE
                     EPTADEPKGDDPKAEEPRAEEPKVEEQAVPEAVEQAPTEAPAPEIPAAEALEAEQAAG
                     DDDATEDSAS"
     gene            complement(370..570)
                     /locus_tag="VLR27_07855"
     CDS             complement(370..570)
                     /locus_tag="VLR27_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03398.1"
                     /translation="MSSDPGQGRLQCPYCDSYEIDRLYLASLRVDSCVCTSCGARWDE
                     DASTGEFRGRQSQARTSRTESR"
     gene            641..1399
                     /locus_tag="VLR27_07860"
     CDS             641..1399
                     /locus_tag="VLR27_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HSP03399.1"
                     /translation="MIEYETRGNVAIITIDRPEARNAVNGDVAEGIEAAIDRMEADDD
                     IWIGVLTHEGPVFCAGADLKAINSGDAARLNTPTGGFGGITAKARTKPIIAACNGPAY
                     AGGCEIVLACDLLVATPDVTFSVPEVKRNLVAAAGALFRLPAALPKNVAMEMLLTGDP
                     IDAERAHALGFVNRMAETGKAVDVAVELAAQISENAPLAVRKSREVALQSFDSDEEAF
                     AASGRAIAEVMASEDTKEGLAAFIEKRKPEWQGR"
     gene            complement(1526..1954)
                     /locus_tag="VLR27_07865"
     CDS             complement(1526..1954)
                     /locus_tag="VLR27_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSP03400.1"
                     /translation="MSDAIPTTSGPEVAVGAVVIDGDRLLLIRRGRGVAIGQWSLPGG
                     VVEPGELLAEAVVRELLEETGLEGVCGSLVGVVERFIEGRHYVILDHHVTLLEPDTTP
                     VAGDDAAEVAWVALSEVADWPLVEGLAEFLHDHGILATIT"
     gene            complement(1951..2442)
                     /locus_tag="VLR27_07870"
     CDS             complement(1951..2442)
                     /locus_tag="VLR27_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP03401.1"
                     /translation="MDVSARPATADDLPALVTLATAAIEELTPNRGGEVWRRHLARPM
                     PPGDSLREDLDDPDAHVVVGAIDGTVIGYGVLRLEHLRDGSTLGVISDLFTLEGARGV
                     SVGEEVMDALIAWATERGCFGVDSLALPGDRHTKNFFESFGLVARAIVVHRSLEPRPD
                     APT"
     gene            complement(2445..3074)
                     /locus_tag="VLR27_07875"
     CDS             complement(2445..3074)
                     /locus_tag="VLR27_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03402.1"
                     /translation="MTADKNPIEEAVDQALDAFVYAPIGLLFDGPARFPKLIRNGRNQ
                     VTNARMMGQFAVQMGRTEVTKRAAELDGPVVDLLRGLGFVDGPPPGPAPAPTPTAVPD
                     KPAAPTAKEGSAKAPAPKKPAAAKKATKKKAVRKATKKTTAKKAAATTTAPDVAGLAI
                     TDYDSLSASQVVSRLQGLSTAELEAVRSYEAATRGRKTILNRVQQLQKP"
     gene            complement(3115..3570)
                     /locus_tag="VLR27_07880"
     CDS             complement(3115..3570)
                     /locus_tag="VLR27_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSP03403.1"
                     /translation="MTYRTIVVGTDGSSSAERAVVQSVDLAAADGARLVIVTAYERTD
                     AKPDERAPADVRWMMADPNEAERHARHARSLATERGVGDVVVQALEGDPADELLEAAD
                     TFDADLIVVGSVGLTGSSRFLLGSVASSVLHHAPCDVLVVHSHGSPRRG"
     gene            complement(3578..4063)
                     /locus_tag="VLR27_07885"
     CDS             complement(3578..4063)
                     /locus_tag="VLR27_07885"
                     /inference="COORDINATES: protein motif:HMM:NF025204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HSP03404.1"
                     /translation="MADEEEDVVDDDLETEDLDEDLDEDIEADDIGEDFESDDDDDVV
                     DVVDDDAVAEEEPTPDAEGDEPRRARRRGEDDEEDEDEDADPDDVEADLDEILKDRIA
                     ATDDEDEDEEEDDGDTGKAAPAGKIQPRREDEFLCQSCFILKPMGQLAEGETELCVDC
                     V"
     gene            complement(4189..4803)
                     /locus_tag="VLR27_07890"
     CDS             complement(4189..4803)
                     /locus_tag="VLR27_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03405.1"
                     /translation="MTAVDTDTEEARPSPAPERRTVPAGAFIIAVLVAGALAVLAVFA
                     LASDDGGSGGGEDQEVRLAAGRFAERFLTFEHDELDAWKDDVLTLSTGGFAEEVEEVE
                     SGLRTLIAESELDAVTQVTDIFVGEVDRGGVEVVVVYDRELRGGEQGRTESDRYLQLA
                     MIRVDGEWLVDNVLDIASAGDLGGSVAPVEGGSGTSTPPTSEGG"
     gene            complement(4800..6020)
                     /locus_tag="VLR27_07895"
     CDS             complement(4800..6020)
                     /locus_tag="VLR27_07895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HSP03406.1"
                     /translation="MSRRVAINLIAFVGVFVLMLWWAVNNIISFDFIERPYEISGEFA
                     ATAGVASNSEVAYLGVNHGSVRSVESVDGKVRITMSIDRGKQIPAGSKARIFRKSAIG
                     EPYIDFEPPEGYEGGGPYIEAGDVIPMERTSVPLEFSELLRSASRVLGSVEPEATRTL
                     IHELAVALDGRGESLRDLTVDSDALLATFAERADLLDSLSANSTRLTRTVTERRASLS
                     SAISDLALVGESLRNVEPDTRILLDRGTELLRTTADLVGDVKQDLDCVLSDLDAVIDV
                     TTTDENLGHLANTLDNGPTGFEYVWLTRDGGPDGLWVRVSILVDTANPAQQYVPPTEL
                     PAVPEIPACVSPLTSAPSGGALAAEAPVEGQAAIAAVPFDTTSASDRSGAALAVFLVS
                     GLVLGLLARKGARA"
     gene            complement(6020..6961)
                     /locus_tag="VLR27_07900"
     CDS             complement(6020..6961)
                     /locus_tag="VLR27_07900"
                     /inference="COORDINATES: protein motif:HMM:NF023315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HSP03407.1"
                     /translation="MTRTAVLRAAVLVVASLLASACTLPGTIRGSREVSAVFDDVGDL
                     VVGHSVQVADVRIGSIVDIELTDDYQAEITMSIKDDVFVPVESRALLRTTSLLGEKFI
                     ELRPLDEDDPMAGTDLVDGDVIAETATAPELEFVTEQAVEILGAVVSSDVATLIETGA
                     VGFGGRTAELTSLVEDLSTVSGTLADQTDELVRIIDALDGATATLASSSGELDELLGN
                     LAETATVLADNRDVAIDAIRELTRLARVQNDNVFEPYLAEVDTQIKQVDAILAEVGRN
                     QAQVAELIQWVSQFADQLPKAVADDFAQVFLLLEVEG"
     gene            complement(6958..8184)
                     /locus_tag="VLR27_07905"
     CDS             complement(6958..8184)
                     /locus_tag="VLR27_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HSP03408.1"
                     /translation="MSPARRHLPRLLAVLLAASLALSGCGLVGGGDTYEVVAYFPRAV
                     SLYESGAVQVLGLPAGEVSDIEIQGDRVKVTLAVDSDIPLPADVRAAIAPQSLIGERR
                     IQLFPPFQQGDQQAQDGHEIAIEDTVIPVEPDEALASLKEFLDALDPNGAGRLIDNAA
                     ASLDGQGQQLGSALDELSDLVGNIEDNDDAIISIAERFDDFTSTLLTRESQLAQVLDD
                     FAVVADVLAEERGEIESLISGLANLSGDGLDLVSEHAVRLRTDVQILQRLTESIEQNL
                     GGVDALIEGGSQQIDGFIDAYNPVSRSLNLRNNFSPLVEEALAPIFESVGIPFPCLPV
                     DVNCASGDLLPLDGERDGAVEADLPAPTTPIDDVLDLLGSPTVAPVAARGADEARTGE
                     RSMLDRFLGTFLGVGS"
     gene            complement(8181..9206)
                     /locus_tag="VLR27_07910"
     CDS             complement(8181..9206)
                     /locus_tag="VLR27_07910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HSP03409.1"
                     /translation="MKSFRDRNPYVVGIISVLLLGGATGFAFLVGTLNLLEDAYPMEG
                     VFTDASGLRAGDDIKLAGVKVGRVTGIDVDRDAGAVNVSWVVDEGTEIRSDARAEIAL
                     SSLLGAKEVRILDAMDGDASMSELPEEQRVIPVERTTVPFDLFELTREATEGVQELDT
                     DALNAFLVDLADISEGKAGTVEDLVEGINRVGAAVNQRDAEFEALLDQADRLSATLAD
                     KDDEILTLLDSSQQILELIVARRNDLALALGESAETVQELDRLISTNKERLDSALDSL
                     APTLDVVAAEQDDIDRALAWLGPGILQQSRGGGHGPWQDIFVRGLGPDVLEGLQDVYS
                     ILLGVDE"
     gene            complement(9203..10294)
                     /locus_tag="VLR27_07915"
     CDS             complement(9203..10294)
                     /locus_tag="VLR27_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HSP03410.1"
                     /translation="MTRTAIKFGLFVAVCLLFTVYLAATIGNTTFGGLVGRGPDTYTL
                     AASFDDVTGLLVDDNVKVAGVPVGKVTGVEVEEGKALVRMEIRSDHQLPSDSAAAIRW
                     RNLIGQRYVYLFPGDAPTTFEDGDVVSETTNVIDLGELFNRLGPVVATIDESQVNDFL
                     ETVTAALEGNEADISQALDDLATVVKGLGERDEAIANLIENLEVVSRTIADRDAQIET
                     MLDNLAALARTFSDNTQLLDSAIVEMGAFNRDLSGLLTANRGELDRIVANLDGTLETV
                     EGQLGPLQLALDRLDDNGRAAFLSSRNGEFLNQTILCVTALPPPCPTPIVTGLDSVVG
                     NSATRQPVAYTPNRRSGGAAVLSLVGGAR"
     gene            complement(10291..11478)
                     /locus_tag="VLR27_07920"
     CDS             complement(10291..11478)
                     /locus_tag="VLR27_07920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HSP03411.1"
                     /translation="MTLTTERNRNMISGAVGLLIVVSLIAIGIKASFGAFDGGYELRG
                     TFAAAGQGLLSGSDVKIRGVNVGEVSSIRLVDNRALLRLRIEDDTQIPETAQAVIRPK
                     TLFGEKFVDILPGEDELDGPYLDDGDEIVDTLGGFELEQVLTDAYPVLEAVNPAELAV
                     VLDELATAGRGLGPNINRSIVNGATLAELGASNDAEFRQFTSDLALLAEELEIIAPDL
                     ISGAEDLNVALPTLNARSAQLDDALTQTARLATDVADLLEANESFTTNALTNGSRALQ
                     TVFDRRSQIQPLLLGVTRYTRTLAEAVRIDVGDGTMMAAVKGVVSVQGLIEGHETPPP
                     DDDPKTPLEELLEGELPELPLPELPVDPVEELDDLLGGSGSSSRTDGGGGLLDLLTGG
                     DDR"
     gene            complement(11483..12340)
                     /locus_tag="VLR27_07925"
     CDS             complement(11483..12340)
                     /locus_tag="VLR27_07925"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP03412.1"
                     /translation="MAAVTKLTRPVQDLGHSVGNGFEDVGKQLSFYGRVLWDVLTTII
                     LRLKYKNVVFSLVSDITVGAGALIVGAGMVFVIFSMSFFTGANVGLQGYAGLEQIGAE
                     AFTGLIGSFANTREVTPLIAGVAFAAQVGAGFTAELGAMRISEEIDALEVMSVRPISY
                     LVGTRVLAALVAIIPLYLIALFASFFATKLVTTEFFGLSPGVYDYYFRLYLPPIDIFY
                     SLVKAAVFAVIVALIHCYYGFYASGGPAGVGVAVGRAIRLSIVAVVVFNLVLSLIFWG
                     GGNTVSLTG"
     gene            complement(12352..13122)
                     /locus_tag="VLR27_07930"
     CDS             complement(12352..13122)
                     /locus_tag="VLR27_07930"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP03413.1"
                     /translation="MIGKVQFVVRETGNMFAISLEGFRRTWDFKAWWREYLGQCWFIA
                     KVCSIPVVLISIPFGAVIALHVGTFSRQLGAESTTGAAMVLAIVREAAPVATALLIAG
                     AGGSAMTADLGSRKIRDELAAMEVMAINPVHRLVTPRLYAAATVALFMVSLVSIAGIS
                     GGYFFNVVIQKVTPGAYFQGFTSLVQLPDLYSSLFKAVVFGFIAASVSCYKGIYAKGG
                     PKGVGDAVNQGVVITFILIFFTNLIMTSLYFALVPPKF"
     gene            complement(13151..14014)
                     /locus_tag="VLR27_07935"
     CDS             complement(13151..14014)
                     /locus_tag="VLR27_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP03414.1"
                     /translation="MDAAISLRNVTKSFGSHTVLQDISFDIPRGKISAVMGPSGTGKS
                     VLLKNVIGLLRPDNGEIWVDGEETVSMGEKDLYRVRRKFGVLFQDGALFGSMNMFDNI
                     AFPLREHTKKSEKEIKEIVLEKASLVGLLDHMKKYPGEVSGGMKKRAGLARALAMDPE
                     IILFDEPDSGLDPVRVSYLDELVKKIQEETGATFLIITHNIASVMRTSDYIAVLYQAS
                     LVKFASKEEMRTTDNPLIRQFLAGRAMGPIGMDELADERTEVEKELMERERRRLEADG
                     RDVDDYGVMTV"
     gene            complement(14249..15415)
                     /locus_tag="VLR27_07940"
     CDS             complement(14249..15415)
                     /locus_tag="VLR27_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HSP03415.1"
                     /translation="MRVPLRPRDIVALITVGVFAIAASGTHVVRSGETLGSIAERNGT
                     TVKALVEANGIANPNLIVAGSTLKIPGGGSAPSTSSGSHTVRSGDTLASIAARYGTTV
                     SALASANGITNPNLIVVGRSLHIQGTGTPSAGSPAAPTSGGKQLPGQRHTVQYGDSIA
                     GIASRYGIRSADLVAWNGLADGKIYATTRLVLFDPGSLPTGGGSSTPGGTHVVQSGQT
                     LSSIASRYGVSSSSIAKANGISDPNRIRVGQTLTVPGSTGSASAGGIVCPVPGATFFN
                     DWGFPRSGGRSHAGTDLFASRGTPVRAPASGHVDTANGKLGGKQFRLTTPAGVVFFGS
                     HLDSFGATGQVSAGDVIGHVGDSGNARGSRPHLHFEVHPNGSAINPFPLLYSSC"
     gene            complement(15554..17158)
                     /locus_tag="VLR27_07945"
     CDS             complement(15554..17158)
                     /locus_tag="VLR27_07945"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HSP03416.1"
                     /translation="MAVYIAEEFTEEEQDILRRYFTNLDGPVFALVNLPEVVKGALFA
                     RYSRSPKSLRRLFLDEFVGDLDITGDQTVDATVGLERAEQLYQKVFLEYGDDSVAQLG
                     GVHLACEQASNILTKILEWGRLAAYLEQSTRYIAYDARLGGRYRYYRDPEVLASPLAL
                     RYVGEMDRLFDAYAELVPVMQEFFRDQFPKAPDDSDLVYRQAIKAKALDAIRGMLPAS
                     SLSNVGIYGTGQSYELLLYRMRSHPLPEARAYADLMLAELRKVIPSFLRRVDLPDRGE
                     VWRDYFGDREEAMAAIADRLLGPDIEPDDVAEVTLVDFDPDAEIKTVASMLYAYSELP
                     ETQLEARVRAMSVEDRLAVIRAYEGDRKNRRHKPGRALERSYYRFDILADYGAFRDLQ
                     RHRMLTLDWQKLTPRHGYVRPPAVDAAGQTAAFDDAMERSATLYDALADDFPEQASYA
                     VALAYKVRFYLHLNARSAMHVLELRSTPQGHPAYRRVAQEMHRLIAEQAGHKAIAESM
                     RFVDHSAEPALERLDSERAAEARRNT"
     gene            complement(17226..18413)
                     /locus_tag="VLR27_07950"
     CDS             complement(17226..18413)
                     /locus_tag="VLR27_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HSP03417.1"
                     /translation="MDLAAVKHRMAEEIDRRAELLLDVSHRIHANPELNFEEHHAHDL
                     LCDVLEDQGIATERRSHGLDTAFVAHAGSGGPNIAVCLEYDALPGIGHACGHNIIAAA
                     GLGAGLAAAAVADACEGRITILGTPAEEGGGGKVLQIRAGAFEGVDAAMMVHPSGADL
                     RGMDTVAIHRCLATYRGESAHAAAAPERGRNALDAAVLGYMNVAALRQHIDPNERIHG
                     VFTDGGEKPNIVPSRAQAEWYVRSPTVARLQELKARVVACLEAGATAAGCEVELDWLE
                     PPYADMVPVEAYCDLYAANAAALGRDVRVPDETCCVVGSTDMGDVSYVVPSIHPMIQA
                     ADPGTAIHTEAFAGFAAGPLGDGAVVDGAKALAWTVADLWMQPGLLDSITAEHREQVA
                     ARR"
     gene            18473..19501
                     /locus_tag="VLR27_07955"
     CDS             18473..19501
                     /locus_tag="VLR27_07955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP03418.1"
                     /translation="MSTIEVHQVTPDRPDLLEALVAVRAVTDREVNPDDPPAPADEVA
                     AELFTEWAMVRHLGFVALVDGTPAGELGLAAEKTEENAHMVDVEWLAVDPAHRRRGVA
                     DALLATGLGWAEDDGRTAAVLWAPTLPGGAGRAYAERCGATLRLEERCSRLPIAELDQ
                     DRQDEWLRAGRDRTDGYRIVQWVGPTPEEHLEALAAAHRAMEDMPTDELDWTVPPMTP
                     ERLRSRDEVWAARGRRNVSTLALAPDGAAAGLSELQINTHRPVLAAQGDTGVVADHRG
                     HGLGRWLKAENLRLALDTEPRIRIVETYNAESNPWMLDINVAMGFRPHVGYQALQGDV
                     SGMRKVVG"
     gene            complement(19511..20497)
                     /locus_tag="VLR27_07960"
     CDS             complement(19511..20497)
                     /locus_tag="VLR27_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSP03419.1"
                     /translation="MDAWILDESPGSYRFGTIDTPEPGYGEVRVRPVTSALNHMDLWL
                     TRGMPKPTLPHVPGCDVAGVIDAVGEAVDLEVGAEVVVNPAVSTMDAVVHLGNDAPVG
                     RGFQILGEQRWGGHADGVVVPARNIVPKPAGRSWEECAAYPLATLTAYRMLRRARLVA
                     GETVLVVGIGGGVSMAALALALRMGAEVYVTSRDQAKVDQAVAMGATAGFDSAEPSWP
                     VKAHVVVESVGPATWDASVRSLVPGGRLAVCGGTSGQKVEVDLPKLFFKQIEIIGSTM
                     GSYEEFDAVTRLVDQGLPVAVDEVFDRADYPDALERLRSGDQLGKVVLRHPS"
     gene            complement(20570..21181)
                     /locus_tag="VLR27_07965"
     CDS             complement(20570..21181)
                     /locus_tag="VLR27_07965"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03420.1"
                     /translation="MTVRKGQPWGEPGALPGDGIVVDSDLAARRVVTDARRANRPIPP
                     LGLLAGDLARTCGATGREDRLRSPEAQTLPVDLGEVLVDGALHFFVAHLVARRSWWRG
                     PIVAAMNAQFLGGWDVAPRSHPGDGRLDLFEADLSLSDRWKARTRLPTGTHVPHPGIS
                     ESRVKAVQLDLAPGTSVWLDGERIGEAKALSIRVAPDALTVVV"
     gene            complement(21178..23838)
                     /locus_tag="VLR27_07970"
     CDS             complement(21178..23838)
                     /locus_tag="VLR27_07970"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSP03421.1"
                     /translation="MTGPVGPHTQALLDSYTFALDGFQRRAADALDAGRSVLVSAPTG
                     AGKTVVADYAVAQAMDRGLRAFYTTPIKALSNQKFGDLRHRYGSGRVGLLTGDNSIDG
                     DAPAVVMTTEVLRNMIYAGRPLDDLAVVVLDEVHYLQDSFRGPVWEEVIVHLPEHVQL
                     VCLSATVSNAHDIGDWLATVRGPTDVVVEETRPVELANTYLVGDKTSDRLHLLDTLVD
                     GRPNPEGERFDIDSRGPTRHGGRQQRGRGRPRSPWYTPNRLDVLDVLEDDELLPAIVF
                     IFSRAACDDAVKGCLDAGVRLTSADEREQIRRIAEDHLTSLTDHDLDALDADRWLAGL
                     EAGVAAHHAGHVPPFKEAVEACFVRGLVKVVFATETLALGINMPARAVVIERMTKFNG
                     ERRELLTPSEYTQLTGRAGRRGIDTHGTAIVLWSPFISFGQIAGLAGSRTFVLRSAFR
                     PTYNMAVNLIRRHDRDEARRLLNQSLAQYQADKAVVVLERRLDKRRQNLRAVQAEATC
                     DLGDVGEYRELLGATAPRRVRRAQLEHSLASLKPGDVIALEDGPRVAVLSVAFRKGSI
                     KVKAVTETREVAVVSMADFDQPPRRLGAVDLPEPYLPEDSDFRGQVASRLARVRVPAR
                     SDHVPDVPAVEHPVAECPDADSHVRAYDRALRLQGEVDELSRRIERQTSTLSLQFDRV
                     LDVLDEFGYVETWSLTDRGRLLAGVYHEADLVVAEAIADGIFDDLDAPELAAVASVLV
                     YEHRAPGPPPAARYPSRRVRERAAEVDSIVRRVQAAEDAVGLPWSRGSDHGFCAVAHE
                     WCAGEGLGDLLEETNLELTGGDFVRTVRQLIDLVRQIAQLAPDDVTRATARRAVGALE
                     RGVVSASASVEDDDEGDGADLDADPTPPSP"
     gene            complement(23835..24602)
                     /gene="tatC"
                     /locus_tag="VLR27_07975"
     CDS             complement(23835..24602)
                     /gene="tatC"
                     /locus_tag="VLR27_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HSP03422.1"
                     /translation="MSDDTTEGRMSMLEHLYELRDRIIKSAIAIAVGAVVAWFLYPQI
                     FEILLDPYCDLQGSSVDDCLLLQTEPLEGFSVRLKIAGYGGIALAMPVLLWQVWRFVT
                     PGLYSHEKKWAYPFVFAGMVLFLLGAGLAFYSLTPALEFLVTVGGEGLNQEFRPAPYF
                     ELITYMMLAFGVGFEFPIVLIFLQLAGIVEAATLRKYRRHAILGIVVLVAVITPSGDP
                     YTLGILSVPMYLFYELAILIGMLLTRKRRREEAALTS"
     gene            complement(24599..24961)
                     /gene="tatB"
                     /locus_tag="VLR27_07980"
     CDS             complement(24599..24961)
                     /gene="tatB"
                     /locus_tag="VLR27_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HSP03423.1"
                     /translation="MFNVGGMELIVIGVVALVVLGPDKLPGAMRQLGTFVGEMRRISR
                     GFQTDLRSALDEAERDAERERLAADQAARGSAPATNQAAGTAPDPGAAMAAAQAELDA
                     IEAVADTTDADADTERDT"
     gene            complement(25002..25211)
                     /locus_tag="VLR27_07985"
     CDS             complement(25002..25211)
                     /locus_tag="VLR27_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03424.1"
                     /translation="MTTSLGMVVAALPLLILAAAMGLIGVAVARSLRDETVLDGLRAE
                     VRSIGEVHRAVHEARASVTGRTTLR"
     gene            complement(25216..25569)
                     /locus_tag="VLR27_07990"
     CDS             complement(25216..25569)
                     /locus_tag="VLR27_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03425.1"
                     /translation="MATRIRASCEECGDVELHVDDVRVRVCIDDDAGSYVFRCPSCTM
                     SVVKDAEPRVVELLVASGVELTAWSIPSELFEPHGGPAFTHDDLLDFHELLEGDDWQR
                     ALLDPAPGGSTTDAG"
     gene            25679..26386
                     /locus_tag="VLR27_07995"
     CDS             25679..26386
                     /locus_tag="VLR27_07995"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HSP03426.1"
                     /translation="MARVASLRQPPIGFAHRGARAHAPENTLDAFALAVKLGATGLES
                     DVWVTADGVAVLDHDGVVRKGVRRRGIAEIRRDELPGHIPSLAELYERCGTELPLSLD
                     VKDPAALEPTVAAARDAGAEEQLWLCHPDWELVATWRRLAEGAKLVDSTRMAKIKEGP
                     ERRAMSLRDAGIDCINMHESDWTGGLTTLFHRFGLECFGWDAQLPRTLRQLIDRGVDG
                     IFSDHVDRMVDALAGRR"
     gene            complement(26396..28411)
                     /locus_tag="VLR27_08000"
     CDS             complement(26396..28411)
                     /locus_tag="VLR27_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSP03427.1"
                     /translation="MAHPELLDEQHHLDRAASALRAMAARTTAALGASQAAATEADSE
                     VAAFHLRRRLESLADRDDALAFGRIDEGPERWYVGRRHVEDDTGTPLVVDWRAPVSVP
                     FYRATVHDPLGLDRRRRFLTEGRDLLDILEEDFSDPDRALLSGGIPDPLLAELERERT
                     GTMRDIVATIAAEQDEVIRAPIDRLLVVQGGPGTGKTAVALHRAAYLLFEHRLELLDR
                     GVLVVGPNQLFLRYVADVLPSLGETAVRQTTVEGLRPATVRVEEGAPEPDAVGRIKGD
                     VRLAEVIDRLVVDAIRVPDEPVAIGTAWGMVRLLPADVAASVEDVLGRGVTHATGKDA
                     LRNQLLHRAYELHERTAEVPALKANFVAAARSDAGFTRHLDRMWPTLAATTVVQRLLT
                     NRAARRRATAGLLSEDEATLLARPLPSRKADVRWWPAEVPLLDEAEARLHGVRTTYGH
                     IIVDEAQDLSAMALRMLARRAHGGSMTVVGDLAQATRAWGQRSWDDVVALLQGSAAPA
                     RAELTVGYRTPAPILDLANRLLPVAAPDVTPARSIRTTGEAPVFVPTPAARLATASAE
                     AVSEVAGERGTVAVVATAAHHDELATALDDAGVAWARGVDGLGSVALITPLLAKGLEF
                     DVVVVVEPAEIVRAEPAGHRALFVALTRPTRRLVIVHAEPLPPALGT"
     gene            28440..28952
                     /locus_tag="VLR27_08005"
     CDS             28440..28952
                     /locus_tag="VLR27_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03428.1"
                     /translation="MAGHGVYPGSFDPLTVAHLAIADAARDQCDLDRLDLVVSHVALA
                     KEDRHQTPPGDRLAHIERATPDRPWLSARDTTAQLLADIADGYDILVVGADKWHQLHD
                     ARFYGGSEAAMRAALARLPRVAVAPRAGVRLPVDDRVTVLDVDPIHHEVSSTGVRAGR
                     LEWRAGGAGP"
BASE COUNT         4132 a         9899 c        10952 g         4106 t
ORIGIN      
        1 aggacgccaa gcccggcgag gccaagggcg agcgcaaggc tgacaagccc aagggtgaca
       61 agcccaaggc cgacgagccc aagggtgacg agccgaaggc tgaggagccc aagcgcgacg
      121 agcccacggc tgaggagccc acggccgacg agcccaaggg tgacgatccg aaggctgagg
      181 agcccagggc tgaggagccg aaggtcgagg agcaggcggt ccccgaggcc gtcgagcagg
      241 cgcccaccga ggcaccggcc ccggagatcc ccgccgccga ggcgctcgag gccgagcagg
      301 ctgcaggcga cgacgacgcc accgaggact cggccagctg aggccaggcg cggcgccgct
      361 ggcgccgcgt cagcgggatt cggtgcggga ggtccgggcc tggctctgcc ggccccggaa
      421 ctcgccggtc gatgcgtcct cgtcccagcg ggctccgcac gaggtgcaca cgcaggagtc
      481 gacccgcaac gacgccaggt agaggcggtc gatctcgtag gagtcgcagt aggggcactg
      541 gagccgcccc tgtcccgggt cgctggacac ggccgcaggg taccgcccga gtgccggtgc
      601 gcatggaagc ctggcgccgg gtcatcgcta ctgtgcgccc atgatcgagt acgagacccg
      661 tggcaacgtc gccatcatca ccatcgaccg tcccgaggcg cgcaacgccg tcaacggcga
      721 cgtggccgag ggcatcgagg ccgccatcga ccgcatggag gccgacgacg acatctggat
      781 cggcgtcctg acccacgagg gtcccgtgtt ctgcgccggc gccgacctca aggcgatcaa
      841 cagcggtgac gccgcccgcc tcaacacccc caccggtggc ttcggcggca tcaccgccaa
      901 ggcccgcacc aagcccatca tcgcggcctg caacgggccc gcgtacgccg gtggctgcga
      961 gatcgtcctg gcctgcgacc tgctggtcgc caccccggac gtcaccttca gcgtgcccga
     1021 ggtgaagcgc aacctggtcg ccgctgccgg cgcgctgttc cgcctgcccg ccgcgctccc
     1081 caagaacgtg gcgatggaga tgctgctcac cggggacccg atcgacgccg agcgggccca
     1141 cgccctcggc ttcgtgaacc gcatggccga gacgggcaag gccgtcgacg tcgccgtcga
     1201 gctggccgcc cagatcagcg agaacgcccc gctcgccgtg cgcaagagcc gcgaggtggc
     1261 gctgcagtcc ttcgacagcg acgaggaggc cttcgccgcc tcgggacggg ccatcgccga
     1321 ggtcatggcc agcgaggaca ccaaggaggg cctggccgcc ttcatcgaga agcgcaagcc
     1381 cgagtggcag gggcgctagc gcaacaccac gactgccagt tcaggagccg agccgccagg
     1441 cgaggcgacc catgagcacg gttccctacg gcggcggagc cgccagaccg agtcacggga
     1501 gggcgttacc agttcgtaca gctcgttacg tgatggtggc gaggatgccg tggtcgtgga
     1561 ggaactcggc caggccctcg accagcggcc agtcggcgac ctcgctgagc gccacccacg
     1621 ccacctcggc cgcgtcgtcg ccggcgacgg gggtggtgtc gggctcgagc agggtcacgt
     1681 ggtggtcgag gatcacgtag tggcgaccct cgatgaagcg ctcgacgacg ccgacgaggg
     1741 agccgcacac gccttcgagg ccggtctcct ccagcagctc gcggaccacg gcttcggcga
     1801 gtagctcgcc gggttcgacc acgccgccgg gcagggacca ctgcccgatg gccacacctc
     1861 ggccgcgccg gatcagcagg agacggtcgc cgtcgatcac gaccgcgccg acggcgacct
     1921 cgggtccgga ggtggtgggg atcgcgtcgc tcatgtcggt gcgtcgggac ggggctcgag
     1981 ggaccggtgc acgacgatgg cccgggccac gaggccgaag gactcgaaga agttcttggt
     2041 gtgccggtcg ccgggcagcg ccagggagtc cacgccgaag cacccccgct cggtcgccca
     2101 cgcgatcagc gcatccatga cctcctcccc caccgagacg ccgcgggcgc cctcgagggt
     2161 gaacaggtcg gagatgacgc cgagcgtgga gccgtcgcgc aggtgctcga gccgcaggac
     2221 cccgtagccg atcacggtgc cgtcgatggc cccgacgacg acgtgggcat cggggtcgtc
     2281 gaggtcctcg cgcagcgagt cgccgggcgg catgggtcgg gccaggtggc gccgccacac
     2341 ctcaccaccc cggttcggag tgagctcttc gatggcagcg gtggccaggg tgaccagcgc
     2401 ggggaggtcg tcggccgtgg ccggacgggc ggagacgtcc acgcctaggg cttctgcagc
     2461 tgctggaccc tgttgaggat ggtcttgcgt ccccgggtcg cggcctcgta gctgcgcacg
     2521 gcctcgagct cggcggtgga gagcccctgc aggcggctca cgacctggct ggccgagagg
     2581 ctgtcgtagt cggtgatggc caggccggcg acgtcgggcg ccgtggtggt cgcggccgcc
     2641 ttcttcgccg tggtcttctt cgtggccttc cggaccgcct tcttcttggt ggccttcttg
     2701 gccgccgccg gcttcttggg agcgggcgcc ttcgcgctgc cctccttggc cgtcggggcg
     2761 gcgggcttgt cggggaccgc cgtcggcgtg ggggccggtg ccgggccggg cggcgggccg
     2821 tcgacgaacc cgagcccgcg cagcaggtcg acgaccggtc cgtcgagctc ggcggcccgc
     2881 ttggtgacct cggtgcgacc catctggacg gcgaactggc ccatcatccg ggcgttcgtc
     2941 acctggttgc ggccgttgcg gatcagcttc gggaaccggg ccggcccgtc gaacagcagc
     3001 ccgatcgggg cgtacacgaa ggcgtcgagc gcttggtcga cggcctcttc gatggggttc
     3061 ttgtcagcgg tcacagccgg ccatgttcgc ggccgggtcc gtcttcgcgc cagttcatcc
     3121 ccttcgcggc gacccgtggc tgtgcaccac gaggacgtcg cagggggcgt ggtgcagcac
     3181 gctgctggcc acgctgccga gcaggaaccg gctgctgccg gtgagaccga ccgagccgac
     3241 cacgatcagg tcggcgtcga aggtgtcggc tgcctcgagc agctcgtcgg cgggatcgcc
     3301 ctcgagcgcc tgcacgacga cgtcgcccac gccgcgctcg gtggccaggg accgggcgtg
     3361 gcgggcgtgg cgctcggcct cgttggggtc ggccatcatc cagcggacgt cggcgggggc
     3421 gcgctcgtcg ggcttggcgt cggtgcgctc gtaggcggtg acgatgacca ggcgggcacc
     3481 gtcggcggcg gccaggtcga ccgactgcac gacggcacgc tcggccgaag aggagccgtc
     3541 ggtgccgacc acgatcgtgc ggtaggtcat ggcgtgctca gacgcagtcg acgcacagct
     3601 cggtctcgcc ctcggcgagc tggcccatcg gcttcaggat gaagcaggac tggcagagga
     3661 actcgtcctc gcgacggggc tggatcttgc ccgccggtgc ggccttgccc gtgtcgccgt
     3721 cgtcttcctc ttcgtcctcg tcctcgtcgt cggtggccgc gatgcggtcc ttgaggatct
     3781 cgtccaggtc ggcctcgacg tcgtcggggt cggcgtcttc gtcctcgtcc tcctcgtcgt
     3841 cctcacctcg ccggcgggcg cgtcggggct cgtcgccctc ggcgtccggg gtcggctcct
     3901 cctcggcgac cgcgtcgtcg tcgaccacgt cgacgacgtc gtcgtcatcg tcgctctcga
     3961 agtcctcgcc gatgtcgtcg gcttcgatgt cctcgtcgag gtcctcgtcg agatcttcgg
     4021 tctcgaggtc gtcgtcgacc acgtcttctt cttcgtcagc cattggcact ccaacgggtg
     4081 tggcgtcggc ggcggatcat agacagcgtc cgtcgcgggg agggttgccg ggtcgcggga
     4141 acctatcggc agtggaccgg gattccacaa caagggccga agcggcgctc agccgccctc
     4201 ggacgtgggc ggtgtcgagg tgccggagcc gccctcgacg ggcgccacgg agcccccgag
     4261 gtcgcctgcg gaggcgatgt cgagcacgtt gtcgaccagc cactcgccgt cgacccggat
     4321 catggcgagc tgcaggtagc ggtcggactc ggtgcggccc tgctccccgc cgcggagctc
     4381 ccggtcgtac acgacgacca cctcgacccc gccccggtcg acctccccga cgaagatgtc
     4441 cgtgacctgg gtcacggcgt cgagctcgct ctcggcgatc agggtgcgca gcccggactc
     4501 gacctcctcg acctcctcgg cgaagccgcc ggtgctgagg gtgagcacgt catccttcca
     4561 ggcgtcgagc tcgtcgtgct cgaacgtgag gaaccgctcg gcgaagcggc cggcagccaa
     4621 gcggacctcc tggtcctcac cgccgccgga gccaccgtcg tcggatgcga gggcgaagac
     4681 ggcgagcacg gcgagggcgc cggcgacgag cacggcgatg atgaaggccc cggcgggcac
     4741 ggtgcggcgc tcgggcgcgg gcgacgggcg cgcctcctcg gtgtcggtgt cgaccgcggt
     4801 catgctcgcg ctcccttccg ggccagcagc ccgagcacca ggccgctcac caggaacacg
     4861 gccagggccg ccccggagcg atcggaggcg ctcgtggtgt cgaacgggac ggcagcgatc
     4921 gccgcctgcc cctccaccgg cgcctcggcc gccagcgcgc cgccggaggg cgctgaggtc
     4981 agcggtgaca cgcaggccgg gatctcgggc acggcgggca gctcggtggg cggcacgtac
     5041 tgctgggccg ggttggcggt gtcgaccagg atgctgaccc gcacccagag cccgtcgggg
     5101 ccgccgtccc tggtgagcca gacgtactcg aacccggtcg gcccgttgtc gagggtgttg
     5161 gcgaggtggc ccaggttctc gtcggtggtc gtgacgtcga tcaccgcgtc gaggtcggac
     5221 agcacgcagt cgaggtcctg cttgacgtcg ccgacgaggt cggcggtggt gcgcagcagc
     5281 tcggtgccgc gatcgagcag gatgcgcgtg tcgggctcga cgttgcgcag cgactccccc
     5341 accagggcga ggtcgctgat ggccgacgac agcgatgccc gccgctcggt gacggtgcgg
     5401 gtcaggcggg tgctgttggc gctgaggctg tcgaggaggt cggcgcgctc ggcgaaggtg
     5461 gccagcagcg cgtcggagtc gaccgtgagg tcgcggaggg actcaccccg gccgtcgagg
     5521 gcgacggcca gctcgtggat cagggtccgg gtcgcctcgg gctcgaccga gccgagcacg
     5581 cgcgacgcgg agcggagcag ctcggagaac tcgaggggca ccgaggtgcg ctccatcggg
     5641 atcacgtcgc cggcttcgat gtagggcccg ccaccctcgt agccctcggg gggctcgaag
     5701 tcgatgtagg gctcgccgat ggccgacttg cggaagatgc gggccttgga gccggccggg
     5761 atctgcttgc cccggtcgat gctcatggtg atgcgcacct tgccgtcgac ggactcgacg
     5821 gagcgcacgg agccgtggtt gacgcccagg taggcgacct cggagttgct ggcgacgccg
     5881 gcggtggcgg cgaactcgcc ggagatctcg taaggccgct cgatgaagtc gaagctgatg
     5941 atgttgttga ccgcccacca cagcatgagg acgaacacgc cgacgaaggc gatgaggttg
     6001 atcgcgaccc gacgggacat cagccctcca cctccaggag caggaacacc tgggcgaagt
     6061 cgtcggcgac ggccttgggc agctggtcgg cgaactggga cacccactgg atcagctcgg
     6121 cgacctgagc ctggttgcgg ccgacctcgg cgaggatggc gtccacctgc ttgatctggg
     6181 tgtcgacctc ggccaggtag ggctcgaaga cgttgtcgtt ctgcacccgg gccaggcggg
     6241 tcagctcccg gatggcgtcg atggcgacgt cgcggttgtc ggccagcacc gtggcggtct
     6301 cggccaggtt gccgagcagc tcgtccagct cgcccgagga cgaggccagc gtggcggtgg
     6361 cgccgtcgag ggcgtcgatg atccgcacga gctcgtcggt ctggtccgcc agggtgcccg
     6421 acacggtgga gaggtcctcg accagcgagg tcagctccgc ggtccggccg ccgaagccga
     6481 cggccccggt ctcgatcagc gtggccacgt cggaggacac gaccgcgccg aggatctcca
     6541 cggcctgctc ggtgacgaac tcgagctcgg gtgccgtcgc ggtctcggcg atcacgtcgc
     6601 cgtcgaccag gtcggtgccg gccatggggt cgtcctcgtc gagcgggcgc agctcgatga
     6661 acttctcccc gagcagtgac gtggtgcgca gcagggctcg cgactcgacc ggcacgaaca
     6721 cgtcgtcctt gatcgacatc gtgatctcgg cctggtagtc gtcggtgagc tcgatgtcga
     6781 cgatcgagcc gatgcggacg tcggcgacct gcacggagtg gccgacgacg aggtcgccga
     6841 cgtcgtcaaa cacggcgctg acctctcggg agccgcggat ggtgccgggc agggtgcacg
     6901 cgctcgccag cagcgaggcg accacgagca cagcggcgcg gagcacggcg gtgcgggtca
     6961 cgaccccacc cccaggaagg tgccgaggaa gcggtcgagc atgctgcgct cccccgtccg
     7021 ggcctcgtcg gcgccgcggg ccgcaaccgg ggcgaccgtg ggcgagccga gcaggtcgag
     7081 cacgtcgtcg atcggggtgg tgggagccgg gaggtcggcc tcgaccgcgc cgtcccgttc
     7141 accgtcgagt ggcagcaggt cgccggaggc gcagttcacg tcgacgggca ggcacgggaa
     7201 cgggatgccc acggactcga agatgggcgc cagggcctcc tcgaccagcg gtgagaagtt
     7261 gttgcgcagg ttcagcgagc ggctcaccgg gttgtaggcg tcgatgaagc cgtcgatctg
     7321 ctgcgacccg ccctcgatga gcgcgtcgac cccaccgagg ttctgctcga tgctctcggt
     7381 gagccgctgc aggatctgga cgtcggtgcg cagccgcacg gcgtgctcgg acacgaggtc
     7441 gagcccgtcg ccggacaggt tggccaggcc ggagatgagg gactcgatct cgccgcgctc
     7501 ctcggcgagg acgtcggcca cgacggcgaa gtcgtcgagg acctgggcga gctggctctc
     7561 gcgggtgagc agggtcgagg tgaagtcgtc gaagcgctcg gcgatgctga tgatggcgtc
     7621 gtcgttgtcc tcgatgttgc cgaccaggtc ggagagctcg tcgagcgcgg agccgagctg
     7681 ctggccctgc ccgtcgagtg aggcggcggc gttgtcgatc agccgtccgg caccgttggg
     7741 gtcgagggca tcgaggaact ccttgaggga ggccagcgcc tcatcgggct ccacggggat
     7801 cacggtgtcc tcgatggcga tctcgtggcc gtcctgggcc tgctggtcgc cctgctggaa
     7861 cggggggaac agctggatgc gccgctcgcc gatgagcgac tgcggcgcga tggccgcccg
     7921 cacgtcggcg ggcagcggga tgtcgctgtc gaccgccagg gtgaccttga cccggtcgcc
     7981 ctggatctcg atgtcggaga cctcaccggc ggggaggccg agcacctgca cggcgccgga
     8041 ctcgtacagg gacaccgccc gcgggaagta ggccacgacc tcgtaggtgt cgccaccgcc
     8101 caccagcccg cagccggaca gggcgagcga ggcggcgagc agcacggcca ggaggcgggg
     8161 caggtgtcga cgtgcggggc tcattcatcc acccccagca ggatcgagta gacgtcctgg
     8221 aggccctcga ggacgtcggg gccgaggccc cggacgaaga tgtcctgcca cggtccgtgc
     8281 ccaccgcccc gcgactgctg gaggatgccc gggccgagcc aggcgagggc gcggtcgatg
     8341 tcgtcctgct ccgcggcgac cacgtcgagg gtgggcgcca gggagtcgag tgccgagtcg
     8401 agccgctcct tgttcgtgga gatcagccgg tccagctcct ggacggtctc ggctgactcc
     8461 ccgagggcga gcgccaggtc gttgcggcgg gcgacgatga gctcgaggat ctgctgggag
     8521 gagtccagca gcgtcaggat ctcgtcgtcc ttgtcggcga gggtggcgct gaggcggtcg
     8581 gcctggtcga gcagggcctc gaactcggca tcgcgctggt tgaccgccgc gccgacgcgg
     8641 ttgatgccct cgacgaggtc ctcgacggtg ccggccttgc cctcggagat gtcggcgagg
     8701 tccacgagga aggcgttgag cgcgtcggtg tcgagctcct ggacgccctc ggtggcctcg
     8761 cgggtgagct cgaagaggtc gaagggcacc gtggtgcgct cgacggggat cacccgctgc
     8821 tcctcgggga gctccgacat ggatgcgtcg ccgtccatgg catcgaggat gcggacctcc
     8881 ttggccccga ggagggagga gagcgcgatc tcggcgcggg cgtcgctgcg gatctcggtg
     8941 ccctcgtcca ccacccagga cacgttgacg gcgccggcgt cgcggtcgac gtcgatgccg
     9001 gtgacccggc cgaccttcac gccggccagc ttgatgtcgt caccggcccg caggccggag
     9061 gcgtcggtga agacgccctc catcgggtag gcgtcctcga gcaggttgag cgtgcccacg
     9121 aggaaggcga agccggtggc cccgccgagg agcagcacgg agatgatgcc gacgacgtag
     9181 gggttgcgat cgcggaacga cttcatcggg cccctcccac gagcgagagc acggcggcgc
     9241 cgccgctgcg gcggttcggc gtgtacgcca ccggctggcg ggtggccgag ttgcccacca
     9301 ccgagtccag cccggtcacg atcggggtcg ggcagggcgg gggcagtgcg gtgacgcaga
     9361 ggatcgtctg gttcaggaac tcgccgttgc gcgaggacag gaaggcggcg cggccgttgt
     9421 cgtcgaggcg gtcgagggcc agctgcagcg gaccgagctg gccctccacg gtctcgagcg
     9481 tgccgtcgag gttggccacg atgcggtcga gctcgccccg gttggcggtg agcagaccgg
     9541 agaggtcgcg gttgaaggca cccatctcga cgatggcgct gtcgagcagc tgggtgttgt
     9601 cgctgaacgt gcgggccagg gcggcgaggt tgtccagcat cgtctcgatc tgggcgtcgc
     9661 ggtcggcgat ggtgcgggac acgacctcga ggttctcgat caggttggcg atggcctcgt
     9721 cccgctcgcc gaggcccttg accacggtgg ccaggtcgtc gagcgcctgg ctgatgtcgg
     9781 cctcgttgcc ctcgagcgcc gcggtgaccg tctcgaggaa gtcgttgacc tggctctcgt
     9841 cgatggtggc cacgacgggt ccgagccggt tgaacagctc ccccaggtcg atgacgttgg
     9901 tggtctcgct gaccacgtcg ccgtcctcga acgtggtcgg cgcgtcgccg gggaacaggt
     9961 agacgtagcg ctggccgatc aggttgcgcc agcggatggc cgccgccgag tccgagggga
    10021 gctggtggtc gctgcggatc tccatgcgca cgagggcctt gccctcctcg acctcgaccc
    10081 cggtcacctt cccgaccggg acgccggcca ccttgacgtt gtcgtccacg agcaggccgg
    10141 tgacgtcgtc gaagctcgcc gcgagggtgt aggtgtcggg gccgcgcccg acgagccccc
    10201 cgaaggtcgt gttgccgatg gtggcggcga ggtacacggt gaacaggagg cagacggcca
    10261 cgaagaggcc gaacttgatg gcggtgcggg tcatcggtcg tcaccgcccg tgagcaggtc
    10321 gagcaggccc ccaccgccgt ccgtgcggga gctgctgccg gagccgccga gcaggtcgtc
    10381 gagctcctcg acggggtcga ccggcagctc gggcaggggc agctcgggca gctcgccctc
    10441 gaggagctcc tcgagcgggg tcttgggatc gtcgtcgggc gggggcgtct cgtggccctc
    10501 gatgaggccc tgcacgctca cgacgccctt gaccgccgcc atcatcgtgc cgtcgccgac
    10561 gtcgatgcgg accgcttcgg ccagggtgcg cgtgtaccgg gtgacgccca ggagcagggg
    10621 ctggatctgg ctgcgccggt cgaagaccgt ctggagggca cgggagccgt tcgtgagcgc
    10681 gttggtggtg aaggactcgt tggcctcgag caggtcggcc acatcggtgg cgagccgggc
    10741 tgtctgggtg agcgcgtcgt cgagctgcgc gctgcgggcg ttcagcgtgg gcagcgccac
    10801 gttgaggtcc tcggccccgg agatcaggtc gggggcgatg atctccagct cctcggccag
    10861 cagggccagg tcgctggtga actggcggaa ctcggcgtcg ttcgaggcgc ccagctcggc
    10921 cagggtggcg ccgttgacga tcgagcggtt gatgttgggc ccgagacccc ggccggcggt
    10981 ggccagctcg tcgagcacga cggcgagctc ggcggggttc accgcctcga ggaccgggta
    11041 cgcgtcggtc aggacctgct cgagctcgaa cccgccgagg gtgtcgacga tctcgtcgcc
    11101 gtcgtcgagg taggggccgt cgagctcgtc ctcgcccggc aggatgtcga cgaacttctc
    11161 accgaagagc gtcttgggcc ggatcacggc ctgcgcggtc tcggggatct gggtgtcgtc
    11221 ttcgatccgg agccggagga gcgcccggtt gtcgaccagg cggatgctgg acacctcgcc
    11281 cacgttcacg ccgcggatct tcacgtcgct gcccgagagg aggccctggc ccgcggcggc
    11341 gaaggtgccg cgcagctcgt agccgccgtc gaaggccccg aagctggcct tgatgccgat
    11401 ggcgatgagg ctcaccacga tcagcaggcc gacggcgccg gagatcatgt tgcggttgcg
    11461 ctcggtggtc agcgtcatgg cgtcatccgg tcaggctcac ggtgttgccg ccgccccaga
    11521 agatgaggga gagcacgagg ttgaagacca ccacggcgac gatggagagc cggatggccc
    11581 ggccgaccgc cacgcccacg ccggcggggc caccgctggc gtagaacccg tagtagcagt
    11641 ggatgagggc gacgatgacg gcgaagacag cggccttcac caggctgtag aagatgtcga
    11701 tcggcggcag gtagaggcgg aagtagtagt cgtagacacc cggcgagagc ccgaagaact
    11761 cggtggtgac gagcttggtg gcgaagaagc tggcgaacag cgcgatcagg tacaggggga
    11821 tgatggcgac gagggcggcg agcacgcggg tgccgaccag gtagctgatc ggacggaccg
    11881 acatgacctc gagggcgtcg atctcctcgg agatgcgcat ggcgccgagc tcggcggtga
    11941 acccggcacc gacctgggcg gcgaaggcca cgccggcgat cagcggggtg acctcccggg
    12001 tgttggcgaa ggagccgatc aggccggtga aggcctcggc cccgatctgc tcgaggccgg
    12061 cgtagccctg cagacccacg ttggcgccgg tgaagaacga catcgagaag atgacgaaga
    12121 ccatgccggc gcccacgatg agcgcaccgg cacccaccgt gatgtcggac accaggctga
    12181 agacgacgtt cttgtacttg aggcgcagga tgatggtggt cagcacgtcc cacagcacgc
    12241 ggccgtagaa cgagagctgc ttgccgacgt cctcgaaccc gttgcccacg gagtggccga
    12301 ggtcctggac gggtctggtg agcttggtga cggcagccat cggggatccg ttcagaactt
    12361 cggcggcacg agcgcgaagt agagggacgt catgatgagg ttggtgaaga agatgaggat
    12421 gaaggtgatg acgacgccct ggttgacggc atcgcccacc cccttgggcc cacccttggc
    12481 gtagatgccc ttgtagcagg acaccgaggc ggcgatgaac ccgaagacca ccgccttgaa
    12541 cagcgacgag tacaggtcgg gcagctgcac cagcgacgtg aagccctgga agtaggcgcc
    12601 cggagtgacc ttctggatga ccacgttgaa gaagtagccg ccggagatgc ccgcgatcga
    12661 cacgagggag accatgaaga gggccaccgt ggccgcggcg tagaggcggg gcgtcaccag
    12721 ccggtgcacc gggttgatgg ccatcacctc catggccgcg agctcgtcgc ggatcttgcg
    12781 ggagccgagg tcggcggtca tggcggaccc accggcgccg gcgatcagca gggccgtggc
    12841 caccggggcc gcctcgcgca cgatggccag caccatggcg gcgcccgtgg tggactcggc
    12901 gccgagctgg cgggagaagg tgccgacgtg gagggcgatc acggcaccga aggggatgct
    12961 gatcagcacc acggggatcg agcacacctt ggcgatgaac cagcactggc cgaggtactc
    13021 gcgccaccag gccttgaagt cccacgtgcg gcggaagccc tcgagggaga tcgcgaacat
    13081 gttccccgtc tcgcggacga cgaactgcac cttgccgatc atcggcggtc cccagacacg
    13141 atccgacgcc tcagaccgtc atgacgccgt agtcgtcgac gtcccgcccg tcggcctcga
    13201 gccgacggcg ctcgcgctcc atcagctcct tctcgacctc ggtgcgctcg tcggccagct
    13261 cgtccatgcc gatggggccc atggcccgac cggcgaggaa ctggcggatg agcgggttgt
    13321 ccgtggtccg catctcctcc ttggaggcga acttcaccag gctcgcctgg tacagcacgg
    13381 cgatgtagtc cgaggtccgc atcaccgacg cgatgttgtg cgtgatgatg aggaaggtgg
    13441 cgccggtctc ctcctggatc ttcttcacca gctcgtccag ataggacacg cgcaccgggt
    13501 cgaggcccga gtcgggctcg tcgaagagga tgatctcggg gtccatcgcc agcgcccggg
    13561 ccaggccggc gcgcttcttc atgccgccgg agacctcacc ggggtacttc ttcatgtggt
    13621 cgagcaggcc gacgaggctg gccttctcga gcacgatctc cttgatctcc ttctccgact
    13681 tcttcgtgtg ctcgcggagc gggaaggcga tgttgtcgaa catgttcatg gagccgaaga
    13741 gggcgccgtc ctggaacagg acgccgaact tgcggcggac ccggtacagg tccttctccc
    13801 ccatcgacac cgtctcctcg ccgtcgaccc agatctcgcc gttgtcgggg cgcaggaggc
    13861 cgatgacgtt cttgagcagc acggacttgc ccgtgcccga cgggcccatg accgccgaga
    13921 tcttgccgcg cgggatgtcg aaggagatgt cctgcagcac ggtgtgggac ccgaagctct
    13981 tcgtcacgtt gcgcagcgag atcgcagcat ccatggggca cctcgggtgg tccggcgagt
    14041 tcggcgacgt tccgggcgac cgcgtcccca gtgacgcacg gcactcccgg gggcgtgacg
    14101 gtacgacaac ggtccgtggc gcgccaagca ctgcgcattt gggacctact gctcaagggg
    14161 ggacgggcga acggattcga gccgatccag ccttcgccgt cgcccgaccg gttccctgcc
    14221 cagggcccgg atcgggcccc gacctgcctc agcaggagct gtagagcagc gggaacgggt
    14281 tgatggccga cccgttggga tgcacctcga agtgcaggtg gggccggctg ccccgggcgt
    14341 tgcccgagtc gccgacgtgg ccgatgacgt cgccggcgct gacctggccg gtggcgccga
    14401 aggagtccag gtggctgccg aagaacacga cgcccgccgg cgtggtgagc cggaactgct
    14461 tgcccccgag cttgccgttg gcggtgtcga cgtgcccgct cgccggggcg cggaccgggg
    14521 tgccgcggga ggcgaacagg tcggtgccgg cgtgggagcg tccgccgctg cgggggaacc
    14581 cccagtcgtt gaagaaggtg gcgccgggca cggggcacac gatgccgccg gcgctggccg
    14641 atccggtcga gccggggacg gtgagggtct ggccgacgcg gatgcggttg gggtccgaga
    14701 tcccgttcgc cttggcgatc gaggacgacg agacgccgta gcggctggcg atcgagctca
    14761 gggtctggcc ggactgcacc acgtgggtgc cccccggggt gctggaccca ccgcccgtgg
    14821 ggagcgagcc gggatcgaac agcacgaggc gggtggtggc gtagatcttg ccgtcggcca
    14881 ggccgttcca ggccaccagg tcggcggaac ggatgccgta gcggctggcg atgccggcga
    14941 tggagtcgcc gtactgcacg gtgtggcgct ggccgggcag ctgcttgccc ccgctcgtcg
    15001 gcgccgccgg gctacctgcg ctcggcgtcc cggtgccctg gatgtgcagg gaccgcccga
    15061 cgacgatgag gttggggttg gtgatcccgt tggcgctggc cagcgccgac accgtggtgc
    15121 cgtagcgggc cgcgatgctg gccagggtgt cgccgctgcg gaccgtgtgg ctcccgctcg
    15181 acgtcgacgg cgcgctgcca ccaccgggga tcttcagcgt ggagccggcc acgatcaggt
    15241 tcgggttcgc gatgccgttg gcctcgacga gtgccttcac ggtggtgccg ttgcgctcgg
    15301 cgatggaccc gagggtctcg ccgctccgga ccacgtgggt cccggaagcg gcgatggcga
    15361 acacgccgac ggtgatcagc gcaacgatgt ctcgtgggcg aaggggtacg cgcacgtgca
    15421 aatgcctcct gcggtctccg cccgctcgga agtgacggca acgtaccgaa gcccctccag
    15481 caactcaacc cccaatctca acatcagcct catgctccac acgcgcgtca ggctggcgca
    15541 cccgtgcggg tgcctaggtg ttgcgcctgg cttcggcggc tcgttcgctg tcgaggcgct
    15601 cgagggcggg ttcggcggag tggtcgacga agcgcatgct ctcggcgatg gccttgtggc
    15661 cggcctgctc ggcgatgagg cggtgcatct cttgggcgac ccggcggtag gcgggatgtc
    15721 cctggggggt cgagcgcagc tcgaggacgt gcatggcgga cctggcgttg aggtggaggt
    15781 agaacctcac cttgtaggcg agggcgacgg cgtagctggc ctgctcgggg aagtcgtcgg
    15841 cgagggcgtc gtacagggtg gccgagcgct ccatggcgtc gtcgaaggcc gcggtctggc
    15901 cggccgcgtc gacagccggc gggcgcacgt agccgtggcg gggggtgagc ttctgccagt
    15961 cgagggtgag catccggtgg cgctgcaggt cgcggaaggc gccgtagtcg gccaggatgt
    16021 cgaagcggta gtacgagcgc tcgagggcac ggcctggctt gtgccgccgg ttcttgcggt
    16081 cgccctcgta ggccctgatc acggcgaggc gatcctcgac cgacatggcc cgcacccggg
    16141 cctcgagctg ggtctcgggc agctcgctgt aggcgtacag catcgaggcg acggtcttga
    16201 tctcggcatc cgggtcgaag tcgacgaggg tgacctcggc gacgtcgtcg ggctcgatgt
    16261 cggggccgag caggcggtcg gcgatggcgg ccatggcctc ctcgcggtcg ccgaagtagt
    16321 cgcgccagac ctcgccacgg tcgggcaggt cgacgcggcg caggaacgac gggatcacct
    16381 tgcgcagctc ggcgagcatg aggtcggcgt aggcccgggc ctcgggcagc gggtgcgagc
    16441 gcatccggta cagcagcagc tcgtagctct gcccggtgcc gtagatgccc acgttggaca
    16501 gcgacgacgc gggcagcatg ccgcggatgg catcgagcgc cttggccttg atggcctggc
    16561 ggtagacgag gtcggagtcg tcgggggcct tggggaactg gtcccggaag aactcctgca
    16621 tcaccggcac cagctcggcg taggcgtcga agagccggtc catctccccc acgtagcgca
    16681 gggccagcgg cgaggcgagg acctcgggat cgcggtagta ccggtagcga ccaccgaggc
    16741 gggcgtcgta ggcgatgtag cgggtggact gctcgaggta ggcggccagg cggccccact
    16801 cgaggatctt ggtgaggatg ttggacgcct gctcgcaggc caggtgcacg ccgccgagct
    16861 gggccaccga gtcgtcgccg tactcgagga acaccttctg gtacagctgc tcggcccgct
    16921 cgagcccgac cgtggcgtcc acggtctggt cgccggtgat gtcgaggtcg ccgacgaact
    16981 cgtcgaggaa caggcggcgc agcgacttgg gcgagcgcga gtagcgggcg aacagcgcgc
    17041 ccttcacgac ctcgggcagg ttgacgaggg cgaacaccgg gccgtccagg ttcgtgaagt
    17101 accgccggag gatgtcctgc tcctcctcgg tgaactcctc ggcgatgtag acggccacgg
    17161 cgggagatgc tacgggctcg gtgcgctccg gccggtgacc actgcgcgtg cccgccgtga
    17221 ggccgtcagc gccgggcggc gacctgctcg cggtgctcgg cggtgatcga gtcgagcagg
    17281 cccggctgca tccacaggtc ggcgacggtc caggccaggg ccttggcccc gtcgaccacg
    17341 gcgccgtcgc cgaggggccc ggcggcgaag ccggcgaagg cctcggtgtg gatggccgtg
    17401 cccgggtcgg cggcctggat catggggtgg atggagggca ccacgtagga cacgtcgccc
    17461 atgtcggtgg agccgaccac gcagcaggtc tcgtccggga cccggacgtc gcgaccgagc
    17521 gcggcggcgt tggcggcgta gaggtcgcag taggcctcga cgggcaccat gtcggcgtac
    17581 gggggctcga gccagtccag ctcgacctcg caaccggccg ccgtggcgcc ggcctccagg
    17641 caggccacga cccgtgcctt cagctcctgc agccgggcga ccgtgggcga ccgcacgtac
    17701 cactcggcct gggccctcga cggcacgatg ttgggcttct cgccaccgtc ggtgaacacg
    17761 ccgtggatgc gctcgttcgg gtcgatgtgc tggcggaggg cggcgacgtt catgtagccg
    17821 agcaccgcgg cgtcgagggc gttgcggcct cgctcgggtg cggcggcggc gtgggcggac
    17881 tcgccccggt aggtggccag gcagcggtgg atggccacgg tgtccatgcc ccgcaggtcg
    17941 gcgccggagg ggtggaccat catcgcggcg tcgacgccct cgaacgcgcc ggcccggatc
    18001 tgcaggacct tgccgccgcc gccctcctcg gcgggcgtgc cgaggatcgt gatgcggccc
    18061 tcgcaggcgt cggcgaccgc ggcggcggcc agaccggcgc cgaggccggc ggcggcgatg
    18121 atgttgtgcc cgcaggcgtg gccgatgccg gggagggcgt cgtactcgag gcacacggcg
    18181 atgttgggtc ccccgctgcc ggcgtgggcc acgaaggcgg tgtcgaggcc gtgggagcgc
    18241 cgctcggtgg cgatgccctg gtcctcgagg acgtcgcaga gcaggtcgtg ggcgtggtgc
    18301 tcctcgaagt tcagctccgg gttggcgtgg atgcggtggg agacgtcgag gagcagctcg
    18361 gcacggcggt cgatctcctc ggccatgcgg tgcttgacgg cggcgaggtc catcgggcga
    18421 tcgtaggggc aattcgctgg agcccggccc ccaccggcgc gacactccgg cgatgtccac
    18481 catcgaggtg caccaggtca ccccggaccg cccggacctg ctcgaggcgc tggtcgccgt
    18541 gcgtgccgtg accgatcgcg aggtcaaccc cgacgacccg ccggccccgg ccgacgaggt
    18601 cgcggcggag ctgttcaccg agtgggcgat ggtgcgtcac ctcgggttcg tggcgctggt
    18661 cgacggcacg cccgccggcg agctgggcct ggccgcggag aagaccgagg agaacgcgca
    18721 catggtcgac gtggagtggc tcgccgtcga cccggcccat cgacgtcgag gggtggccga
    18781 cgccctgctg gccaccgggc tcgggtgggc cgaggacgac ggccggaccg ccgccgtgct
    18841 gtgggcaccg acgctgcccg gcggcgccgg tcgtgcctac gccgagcgct gcggggccac
    18901 gctgcgcctg gaggagcggt gcagccgcct gccgatcgcc gagctcgacc aggaccgcca
    18961 ggacgagtgg ctgcgggccg gacgggaccg caccgacggc taccgcatcg tgcagtgggt
    19021 cggaccgacg ccggaggagc acctcgaggc gctcgccgcg gcccaccgcg ccatggagga
    19081 catgccgacc gacgagctgg actggacggt gccgccgatg acgccagagc ggctgcggtc
    19141 ccgggacgag gtctgggcgg cgcggggccg gcgcaacgtg tccaccctcg ccctcgcccc
    19201 cgacggcgcc gctgctgggc tgtccgagct gcagatcaac acccaccgcc ccgtgctcgc
    19261 cgcccagggc gacaccgggg tggtcgccga ccaccggggc cacggcctcg gccggtggct
    19321 gaaggccgag aacctccgtc tggcgctgga caccgagccg cggatccgga tcgtggagac
    19381 ctacaacgcc gagtccaacc cgtggatgct ggacatcaac gtcgccatgg ggttccggcc
    19441 ccacgtcggg taccaggccc tccagggcga cgtcagcggc atgcggaagg tcgtgggctg
    19501 accgccgagg tcaggacggg tggcgcagca cgaccttgcc gagctggtcc ccgctgcgaa
    19561 gtcgctcgag cgcgtcgggg tagtcggcgc ggtcgaacac ctcgtcgacc gcgaccggaa
    19621 ggccctggtc gaccaggcgg gtgacggcgt cgaactcctc gtaggagccc atggtcgagc
    19681 cgatgatctc gatctgcttg aagaacagct tgggcaggtc gacctcgacc ttctggccgg
    19741 aggtgccccc gcacacggcg aggcggccac caggcaccag cgagcggacc gatgcatccc
    19801 aggtcgcggg gccgacggac tccacgacca cgtgggcctt caccggccag ctcggctcgg
    19861 cggagtcgaa cccggcggtg gcgcccatgg cgacggcctg gtcgaccttg gcctggtcgc
    19921 gggaggtcac gtagacctcg gcgcccatgc gcagcgccag ggccagcgcc gccatggaga
    19981 cgccgccgcc gatgccgacg acgagcacgg tctcccccgc caccagccgc gcccggcgca
    20041 gcatgcggta ggcggtgagc gtggccagcg ggtaggcggc gcactcctcc cagcttcggc
    20101 cggccggctt cgggacgatg ttgcgggcgg gcaccaccac cccgtcggcg tggccgcccc
    20161 agcgctgctc gccgaggatc tggaacccgc gccccaccgg cgcgtcgttg ccgaggtgca
    20221 ccaccgcgtc catggtcgac accgccgggt tcaccacgac ctcggccccg acctcgaggt
    20281 cgacggcctc ccccaccgcg tcgatcacgc cggccacgtc gcagccgggc acgtggggca
    20341 gcgtcggctt cggcatgcca cgggtgagcc acaggtccat gtggttcagg gccgaggtca
    20401 cgggccgcac ccgcacctcg ccgtaccccg gctcgggggt gtcgatggtg ccgaaccggt
    20461 agctgccggg cgactcgtcc aggatccagg cgtccatgac cgccgactct agagacgccc
    20521 gcgagtcgtg aggccctgcc gagcgactcg gggtcgccga aggcgacacc tagacgacga
    20581 cggtgagcgc gtcgggggcg acccggatcg agagggcctt cgcctcgccg atgcgctcgc
    20641 cgtcgagcca caccgacgtg ccgggggcca gatcgagctg cacggccttc acccggctct
    20701 cgctgatccc ggggtgcggg acgtgcgtgc ccgtgggcag gcgggtgcgg gccttccacc
    20761 gatcactgag ggacaggtcg gcctcgaaca ggtcgaggcg gccgtcgccg gggtgggacc
    20821 ggggggccac gtcccacccg cccaggaact gggcgttcat ggccgccacg atcggccccc
    20881 gccaccacga ccgccgggcg acgaggtggg cgacgaagaa gtgcagcgcg ccgtcgacga
    20941 ggacctcgcc gaggtcgacc ggcagcgtct gggcctccgg gctgcgcagc cggtcctcgc
    21001 gcccggtggc gccgcacgtg cgggccaggt cgccggcgag gaggccgagg ggcgggatcg
    21061 gtcggttggc gcggcgggcg tcggtgacca cccggcgggc tgcgaggtcg gagtccacca
    21121 cgatcccgtc acccggcagg gcgcccggtt cgccccacgg ctggcccttg cggacggtca
    21181 cggggacggg ggcgtcggat ccgcgtccag gtccgctccg tcgccctcgt cgtcgtcctc
    21241 gaccgaggcg gaggccgaca cgaccccgcg ctcgagggcg cccacggcgc gacgcgcggt
    21301 ggcccgggtg acgtcgtcgg gggcgagctg ggcgatctgg cggacgaggt cgatcagctg
    21361 gcgcacggtg cggacgaagt cgcccccggt gagctccagg ttggtctcct cgagcaggtc
    21421 gccgaggccc tcgcccgcgc accactcgtg ggcgacggcg cagaacccgt ggtcgctgcc
    21481 ccgcgaccag ggcaggccca cggcgtcctc ggccgcctgc acccggcgca cgatggaatc
    21541 gacctcggcg gcgcgctcgc ggacccggcg cgacgggtag cgagccgccg gcggcggacc
    21601 cggggcgcgg tgctcgtaca cgaggacgct ggcaacggcg gccagctcgg gagcgtcgag
    21661 gtcgtcgaag atcccgtcgg cgatggcctc ggccaccacc aggtcggcct cgtggtacac
    21721 gccagccagg agccggcccc ggtcggtgag cgaccaggtc tcgacgtagc cgaactcgtc
    21781 caggacatcg agcacccggt cgaactgcag gctcagcgtg ctcgtctggc gctcgatccg
    21841 ccgggacagc tcgtccacct cgccctggag ccggagggcg cggtcgtagg cgcgcacgtg
    21901 ggagtcggcg tcggggcact cggcgaccgg gtgctcgacc gcggggacgt cgggcacgtg
    21961 gtccgagcga gcgggcacgc ggacccgcgc caggcgcgat gccacctggc cgcggaagtc
    22021 gctgtcctcg ggcaggtagg gctcgggcag gtcgaccgcc cccagccgcc tcggcggctg
    22081 gtcgaagtcg gccatgctca ccaccgcgac ctcgcgggtc tcggtgacgg ccttgacctt
    22141 gatgctgccc ttgcggaagg cgaccgagag caccgcgacg cggggtccgt cctccagggc
    22201 gatcacgtcg cctggcttga gggaggcgag cgagtgctcg agctgggccc gccgcacccg
    22261 gcggggcgcg gtcgcgccga gcagctcgcg gtactcgccc acgtcgccga ggtcgcaggt
    22321 ggcctcggcc tgcacggcgc gcaggttctg gcggcgcttg tcgagccggc gctccagcac
    22381 gaccacggcc ttgtcggcct ggtactgcgc cagcgactgg ttgagcaggc gacgggcctc
    22441 gtcccgatcg tggcgccgga tcaggttgac ggccatgttg taggtggggc ggaacgccga
    22501 ccgcagcacg aaggtgcggc tgccggccaa gccggcgatc tgaccgaagc tgatgaacgg
    22561 cgaccacagc acgatggcgg tgccgtgcgt gtcgatgccg cgccggccgg cccggccggt
    22621 cagctgggtg tactccgacg gcgtgagcag ctcgcggcgc tcgccgttga acttcgtcat
    22681 ccgttcgatg accacggcgc gggccggcat gttgatgccg agcgcgaggg tctcggtggc
    22741 gaacaccacc ttgaccaggc cgcgcacgaa gcacgcctcg actgcctcct tgaacggcgg
    22801 cacgtgcccg gcgtggtggg cggcgacgcc ggcttcgaga ccggccagcc agcggtcggc
    22861 atcgagggcg tcgaggtcgt ggtcggtgag cgaggtgagg tggtcctcgg cgatgcggcg
    22921 gatctgctcg cgctcgtcgg cggaggtcag gcgcacgccg gcatcgaggc accccttgac
    22981 cgcgtcgtcg caggccgccc ggctgaagat gaacacgatg gccgggagga gctcgtcgtc
    23041 ctcgagcacg tcgagcacgt ccagccggtt cggcgtgtac cagggactcc ggggccggcc
    23101 cctcccccgc tgctgtcggc caccgtgccg cgtcgggccg cgcgagtcga tgtcgaagcg
    23161 ctcgccctcc gggttgggac ggccgtcgac gagcgtgtcc agcaggtgca accggtcgct
    23221 cgtcttgtcg ccgacgaggt aggtgttggc cagctccacc ggtcgggtct cctccaccac
    23281 cacgtcggtg gggccccgca ccgtggccag ccagtcgccg atgtcgtggg cgttggacac
    23341 cgtggccgac aggcacacga gctgcacgtg ctcgggcagg tggacgatga cctcctccca
    23401 caccggcccg cggaagctgt cctggaggta gtgcacctcg tcgaggacga ccacggccag
    23461 gtcgtcgagc ggacgaccgg cgtagatcat gttgcgcagc acctcggtgg tcatgaccac
    23521 cgccggggca tcgccgtcga tcgagttgtc acccgtgagc agcccgacgc gcccgctccc
    23581 gtaccggtgc cggaggtcgc cgaacttctg gttggagagc gccttgatgg gggtggtgta
    23641 gaaggcccgc aggccccgat ccatcgcctg ggccaccgcg tagtccgcca ccacggtctt
    23701 gcccgcgccc gtcggcgcgc tcacgagcac cgagcgcccg gcgtcgaggg cgtcggccgc
    23761 ccgccgctgg aagccgtcca gcgcgaacgt gtagctgtcc aggagggcct gcgtgtgggg
    23821 gccgacggga ccggtcatga cgtgagggcc gcctcctccc ggcggcgctt ccgcgtcagc
    23881 agcatcccga tgaggatcgc gagctcgtag aagaggtaca tcgggaccga caggatcccc
    23941 agcgtgtacg ggtcgcccga cggggtgatc accgccacga gcacgacgat gccgaggatg
    24001 gcgtgccgcc ggtacttgcg cagcgtggcg gcctccacga tgccggcgag ctgcaggaag
    24061 atcagcacga tcgggaactc gaagccgacg ccgaaggcca gcatcatgta ggtgatcagc
    24121 tcgaagtacg gcgcaggccg gaactcctgg ttgaggccct cgccgcccac ggtgacgagg
    24181 aactcgaggg ccggggtcag cgagtagaag gcgagcccgg cgccgagcag gaacagcacc
    24241 atcccggcga acacgaacgg gtacgcccac ttcttctcgt gcgagtagag ccccggggtc
    24301 acgaagcgcc agacctgcca cagcagcacg ggcatggcga gggcgatgcc gccgtacccc
    24361 gcgatcttca gccggaccga gaagccctcg agcggctcgg tctgcaggag gaggcagtcg
    24421 tcgaccgagc tgccctggag gtcgcagtac gggtcgagga ggatctcgaa gatctggggg
    24481 tagaggaacc aggcgacgac ggccccgacc gcgatggcga tggcgctctt gatgatccgg
    24541 tcgcgcagct cgtagaggtg ctcgagcatc gacatgcgac cctcggtggt gtcatcgctc
    24601 acgtgtcccg ctcggtgtcg gcgtcggcgt cggtggtgtc ggcgacggct tcgatcgcgt
    24661 cgagctcggc ctgcgcggcg gccatcgccg caccggggtc gggcgcggtc ccggccgcct
    24721 ggttggtggc cggggcgctg ccccgggcgg cctggtcggc ggcgaggcgc tcgcgctcgg
    24781 cgtcacgctc ggcttcgtcg agtgcgctgc ggaggtcggt ctggaagccc cgcgagatgc
    24841 ggcgcatctc gccgacgaag gtgccgagct ggcgcatcgc gccggggagc ttgtcgggac
    24901 cgaggaccac gagagcgacg acgccgatga cgatcagctc catgccgccg acgttgaaca
    24961 tggcgcggag tgtaccgacg ggcccaggac ccccggcttc gtcagcgcag ggtcgtgcgg
    25021 cctgtcacgg aggcccgggc ctcgtgcacg gcacggtgca cctcgccgat gctgcggacc
    25081 tcggcccgga ggccgtcgag caccgtctcg tcccgcagcg agcgggcgac ggcgacgccg
    25141 atgagcccca tcgccgcagc caggatcagc aggggcaggg ccgcgacgac catccccagg
    25201 gaggtggtca cgacgtcagc cggcgtcggt ggtcgacccg ccgggggcgg ggtcgagcag
    25261 cgcgcgctgc cagtcgtcac cctcgagcag ctcgtggaag tcgagcaggt cgtcgtgggt
    25321 gaacgccgga ccgccgtggg gctcgaacag ctcggacggg atcgaccagg cggtgagctc
    25381 gacgcccgag gcgaccagca gctccaccac ccgtggctcg gcgtccttga cgaccgacat
    25441 ggtgcagctc gggcaccgga agacgtagga gccggcgtcg tcgtcgatgc agacccgcac
    25501 ccggacgtcg tccacgtgca gctcgacgtc gccgcactcc tcacagctcg ctctgatgcg
    25561 tgtcgccatc gtggtcccct cccaggtccc tcgctctcct gcaccaacca tcggcagcga
    25621 tggccgggca cttgagccga ttccctcgcg ttctgccggt cctgcttggc tggccgccat
    25681 ggctcgggtc gcctccctcc gacagccgcc catcggcttc gcccaccggg gcgcccgggc
    25741 ccacgccccg gagaacaccc tcgatgcctt cgccctggcc gtgaagctcg gcgccaccgg
    25801 cctggagagc gacgtgtggg tcaccgccga cggcgtggcc gtcctcgacc acgacggggt
    25861 cgtccgcaag ggcgtccggc ggcggggcat cgccgagatc cggcgtgacg agctgcccgg
    25921 gcacatcccg tccctcgccg agctgtacga gcggtgcggc accgagctgc cgctgtccct
    25981 ggacgtgaag gaccccgccg ccctggaacc caccgtggcc gccgcccgcg acgccggggc
    26041 tgaggagcag ctgtggctct gccacccgga ctgggagctg gtggccacgt ggcgccgcct
    26101 ggccgagggc gccaagctcg tcgactcgac ccgcatggcc aagatcaagg agggaccgga
    26161 gcgtcgagcg atgtcgctgc gggacgccgg catcgactgc atcaacatgc acgagtcgga
    26221 ctggaccggc gggctgacca cgctgttcca ccggttcggc ctcgagtgct tcggctggga
    26281 cgcccagctg ccccggacgc tgcgccagct catcgaccgc ggcgtcgacg gcatcttctc
    26341 cgaccacgtc gaccgcatgg tcgacgccct cgccggccgc cgctgacagc ggccctcagg
    26401 tccccagcgc gggcgggagg ggttcggcgt ggacgatcac cagccgccgg gtcgggcggg
    26461 tgagggccac gaacagggcc cggtggccgg cgggctcggc ccggacgatc tcggccggtt
    26521 cgaccaccac gacaacatcg aactcgaggc ccttggccag cagcggggtg atcaaggcga
    26581 ccgacccgag cccgtcgacc ccgcgtgccc aggcgacgcc ggcgtcgtcc agcgccgtgg
    26641 cgagctcgtc gtggtgcgcg gcggtggcga cgacggcgac cgtgccccgc tccccggcga
    26701 cctcgctgac cgcctcggct gacgcggtgg cgaggcgggc cgcaggggtg ggcacgaaga
    26761 cgggtgcctc gccggtcgtg cggatcgatc gcgccggcgt gacgtcgggg gcggcgaccg
    26821 gcagcagccg gttggcgagg tcgaggatcg gcgcgggcgt ccggtagccg acggtgagct
    26881 cggcccgggc gggcgccgcc gacccctgca gcagggcgac gacgtcgtcc cagctgcgct
    26941 gcccccaggc ccgggtggcc tgggccaggt cgcccaccac ggtcatcgag ccgccgtgcg
    27001 cccggcgggc cagcatccgc agcgccatcg ccgacaggtc ctgggcctcg tccacgatga
    27061 tgtgcccgta cgtggtgcgc acgccgtgca gacgggcttc ggcttcgtcg agcaggggca
    27121 cctcggccgg ccaccagcgg acgtcggcct tgcgggacgg gagcgggcgg gccagcagcg
    27181 tggcctcgtc ctcgctgagg aggcctgcgg tcgcccggcg gcgcgcggcg cggttggtga
    27241 gcaggcgctg caccaccgtg gtggcggcca gcgtcggcca catccggtcc aggtggcggg
    27301 tgaagccggc atcgctgcgg gcggcggcca cgaagttggc cttcagggcc ggcacctcgg
    27361 cggtccgctc gtgcagctcg taggcgcggt gcagcagctg gttccgcagc gcgtccttcc
    27421 cggtggcgtg ggtgacccct cgaccgagca cgtcctcgac ggacgcggcg acgtcagccg
    27481 ggaggaggcg caccatcccc cacgccgtcc cgatcgccac cggttcgtcc gggacccgga
    27541 tggcgtcgac cacgaggcgg tcgatgacct cggcgaggcg gacgtccccc ttgatccgac
    27601 cgaccgcatc cggttcgggc gccccctcct cgacccggac cgtcgccggc cgcaggccct
    27661 cgacggtggt ctggcgcacg gccgtctccc ccaggctcgg gagcacgtcg gccacgtagc
    27721 gcaagaagag ctggttgggg ccgaccacga gcacgccacg gtcgagcagc tcgaggcggt
    27781 gctcgaagag caggtaggcg gcgcggtgga gcgcgacggc cgtcttcccg gtgccggggc
    27841 cgccctgcac cacgaggagg cggtcgatcg gtgcccggat cacctcgtcc tgctcggcgg
    27901 cgatggtcgc gacgatgtcg cgcatcgtcc cggtgcgctc ccgctcgagc tcggcgagca
    27961 gcgggtcggg gatgccgccg gagagcagcg cccggtcggg gtcggagaag tcctcctcga
    28021 ggatgtcgag caggtcccga ccctcggtca ggaagcgacg ccggcggtcg aggccgagcg
    28081 ggtcgtgcac ggtcgcccgg tagaacggaa ccgacaccgg tgctcgccag tcgacgacca
    28141 gcggcgtgcc ggtgtcgtcc tcgacgtgcc ggcgtcccac gtaccagcgc tcggggccct
    28201 cgtcgatgcg cccgaaggcc agcgcgtcgt cacggtcggc cagcgactcg agccgccggc
    28261 ggaggtggaa cgccgccacc tcgctgtcgg cctcggtggc ggccgcctgc gacgcgccga
    28321 gcgctgcggt ggtgcgcgcc gccatcgccc gcagggcgga cgcggcacga tcgaggtggt
    28381 gctgctcgtc gagcagctcg ggatgggcca tggggggcgg caaccgtaac ctcggcgtcg
    28441 tggccggtca cggcgtttac ccggggtcct tcgatccgct caccgtcgcc cacctggcga
    28501 tcgccgatgc ggcacgcgac cagtgcgatc tcgaccggct ggacctggtc gtctcccacg
    28561 tggccctggc caaggaggac cgccaccaga ccccaccggg cgaccgcctg gcccacatcg
    28621 agcgggccac gccggatcgg ccgtggctgt cggcccgcga caccaccgcg cagctcctcg
    28681 ccgacatcgc cgacggctac gacatcctcg tcgtcggggc ggacaagtgg caccagctcc
    28741 acgacgcccg cttctacggc ggctccgagg cggcgatgcg ggcggcgctc gcccgcctgc
    28801 cacgggtggc cgtggccccg cgcgccggtg tgcggctccc cgtcgacgac cgcgtcaccg
    28861 tcctcgacgt ggacccgatc caccacgagg tgagctccac cggtgtgcgc gcgggtcgcc
    28921 tcgagtggcg ggccggcggt gccggtccct agagggtgcc gagctcggtc agcggccaga
    28981 tgatcacgaa cgcccggccg accacgtcgc tggtcggcac ggccttgaac cgccgcgagt
    29041 cgtcggacgc gccccggttg tcgcccatca cccagacgtg gtcctcggg
//
LOCUS       DATISU010000192         4347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_568404, whole genome shotgun sequence.
ACCESSION   DATISU010000192 DATISU010000000
VERSION     DATISU010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568404"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VLR27_08010"
     CDS             <1..640
                     /locus_tag="VLR27_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSP03429.1"
                     /translation="NYVLRTVPAGLVLGVLLAVAANRRLRGIKVFPTIFSSTVATSVA
                     VASIVWLVLINPTVGYFEVDWLRDPDRALFGVSLSSIWQNLGLTFIIVLAGLQAIPDE
                     INEAATVDGMSATRRFFTVTLPMLSPTLLFLVVVLTVFAFQAFAQIDILTNGGPAGST
                     ETLVFKIFQQQQGGAVGEGSVMAVGLFVITFLVALGQFLLLERRVHYGSDRA"
     gene            637..1560
                     /locus_tag="VLR27_08015"
     CDS             637..1560
                     /locus_tag="VLR27_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSP03430.1"
                     /translation="MSDPTTDGDLVVEPATPGLGDVPATSTPAVRPGSRARVAKQVGW
                     YLALTLLSLIVLFPVWMILVRALSQPIAYLTAGQPPRPVEPEWDVFSRAFRQGDLDRR
                     LLTSAGMTIIIATAQVVTSVLAAYAFAFLRFPLRRLAFAAFMATLMLPIEVTLIANVE
                     TIRTLGWFNSFQGLTAPFLAAAFGTFLIRQGFLGIPDDLRDAAELDGYGHLGFLWHVA
                     VPVTRPIIASFTVISFLSAWNQYLWPRSIITREAWDTIQIALPKFASENINQLNLGFA
                     AAIIAALPILAILIFFQRHLIRGLTAGAVKG"
     gene            1586..3058
                     /locus_tag="VLR27_08020"
     CDS             1586..3058
                     /locus_tag="VLR27_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSP03431.1"
                     /translation="MTPRTPRPHGLLAALLAAVLLVAACGGDSGETDDQAAGGDTDGG
                     DSTSCPVDALEDAEGPVDVTLWHAYVGLTNETLNDMADAYNASQDRVRIQVENQGASY
                     EELLRKYRQGIPSRELPGIGIMEDTNTQFLADSGTIVPAGDCAEADGYDGYEDFAPGV
                     VDFYSVDGVLQPASFNVSTVLLYYNRDHFEAAGLDPDSPPQTLDEMIEYARQIKAAGV
                     VDRPVVLNMQPWFIEFWLTGAGAGLVGNDNGRGEGATTESAFDNEATRDLFQKLKTMQ
                     DDGLLNPVPGTEGQFDHYFAIGLEQSSMTIETSTAVTTINSVLEGNLDPAELGIEGEL
                     PPIDVNVDAGPFPGLEGPGEGQLGGGVWYITNTTPPEVQAAAWDFIKYVNEPENQAQW
                     HLQGSYLPVRQATVDSAEVQDFWANTRPGRWLQVAYEGLQKVDPDHPGPQIGPYTETR
                     SAVRSALDALLFGGVGVDGAITEADRTITEALESYADENF"
     gene            complement(3068..3352)
                     /locus_tag="VLR27_08025"
     CDS             complement(3068..3352)
                     /locus_tag="VLR27_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03432.1"
                     /translation="MNAAAAARRRHPSMRAPVDTASVRRRVASVVGSSGGRHPEVAAS
                     VLAVRGASGLDPDAFARRAGVDVEVLRRAEAGEIRRDQLPGSLRRMVPAE"
     gene            complement(3349..4041)
                     /locus_tag="VLR27_08030"
     CDS             complement(3349..4041)
                     /locus_tag="VLR27_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03433.1"
                     /translation="MSPAPASPTPPTSTDPFTVEAIMARLAAGDTAAIFALAEHHGHR
                     IAGVVRRQLRTCGVESVAPDDLQSLVLDACMALHGVARAWRPGGALPWWWAEGRIRSV
                     VNRWVGIHADPLDDHWSRADREVEPAAPSAEEGLAEAFARLVAEDPRVQLVAEAARAA
                     KVDDVALVCLLDYQIQQVQGDPSPAHTLAERYGTTPDAMRQRICRGRRRLRQVVATDP
                     RFAVLSELELVA"
BASE COUNT          655 a         1588 c         1470 g          634 t
ORIGIN      
        1 caactacgtg ctgcgcaccg tgcccgccgg cctggtgctg ggcgtgctcc tcgcggttgc
       61 cgcaaaccgg cggctgcggg gcatcaaggt cttccccacg atcttctcgt ccacggtggc
      121 cacgtcggtg gccgtcgcat ccatcgtctg gctcgtcctg atcaacccga cggtcggcta
      181 cttcgaggtc gactggctcc gcgaccccga ccgggcgctg ttcggcgtgt cgctcagctc
      241 catctggcag aacctcggcc tcacgttcat catcgtgctc gccggcctcc aggccatccc
      301 cgacgagatc aacgaggccg cgacggtcga cggcatgagc gcgacgaggc ggttcttcac
      361 cgtcacgctc ccgatgctct cccccaccct gctgttcctc gtggtggtgc tcacggtgtt
      421 cgccttccag gcgttcgccc agatcgacat cctcaccaac ggcgggccgg ccggctcgac
      481 cgagacgctg gtgttcaaga tcttccagca gcagcagggc ggtgccgtcg gcgagggatc
      541 cgtcatggcc gtgggcctgt tcgtcatcac gttcctcgtg gcgctcggcc agttcctgct
      601 cctcgagcgt cgggtgcact acgggagcga ccgggcatga gcgacccgac gacggacggc
      661 gacctggtcg tggaaccggc aacgccgggc ctcggcgacg tgccggcgac gtcgaccccg
      721 gcggtgcgtc ccggcagccg tgcccgggtc gccaagcagg tcgggtggta cctcgccttg
      781 accctgctct cgctgatcgt gctgttcccc gtctggatga tcctggtccg ggcgctgtcc
      841 cagcccatcg cctacctgac cgccgggcag ccgccccgcc cggtggagcc cgagtgggac
      901 gtgttctccc gtgcgttccg ccagggcgac ctcgaccgcc ggctgctgac ctcagcgggc
      961 atgacgatca tcatcgccac cgcacaggtc gtcacctcgg tgctcgccgc ctacgcgttc
     1021 gcgttcctcc ggttcccgtt gcggcgactc gcgttcgcgg ccttcatggc gacgttgatg
     1081 ctgccgatcg aggtgacgct catcgccaac gtggagacga tccggacgct cggctggttc
     1141 aactccttcc aggggctgac ggcgcccttc ctcgccgccg cgttcggcac cttcctcatc
     1201 cgccagggct tcctcggcat ccccgacgac ctgcgcgacg ccgccgagct cgacggctac
     1261 ggccacctcg ggttcctctg gcacgtggcc gtgcccgtca cccgtccgat catcgcctcg
     1321 ttcacggtga tctcgttcct gtcggcctgg aaccagtacc tgtggccccg ctcgatcatc
     1381 acccgcgagg cgtgggacac gatccagatc gcgctgccga agttcgcgag cgagaacatc
     1441 aaccagctca acctgggctt cgcggccgcc atcatcgccg cgctgccgat cctcgccatc
     1501 ctcatcttct tccaacgtca cctgatccga ggcctgaccg ccggtgcggt caagggctga
     1561 cgacctccca tcccaggagc ttgcgatgac cccccgaacc cctcgccccc acggactgct
     1621 ggctgcgctg ctggcggccg tcctgctcgt cgcggcctgc ggcggcgact ccggcgagac
     1681 cgacgaccag gcggctggcg gggacaccga cggcggcgac agcacctcct gccccgtcga
     1741 cgcgctcgag gacgcagagg gccccgtgga cgtcacgctc tggcacgcgt acgtcgggtt
     1801 gaccaacgag acgctgaacg acatggccga cgcctacaac gcgtcgcagg accgggtgcg
     1861 catccaggtc gagaaccagg gcgcctccta cgaggagctg ctgcgcaagt accgccaggg
     1921 catccccagt cgggagctgc ccggcatcgg gatcatggag gacacgaaca cccagttcct
     1981 ggccgactcc gggaccatcg tgcccgccgg cgactgcgcc gaggccgacg gctacgacgg
     2041 ctacgaggac ttcgcccccg gggtcgtcga cttctacagc gtcgacggcg tcctccagcc
     2101 tgcgtcgttc aacgtctcga ccgtgctgct gtactacaac cgcgaccact tcgaggccgc
     2161 cggcctcgac ccggacagcc cgccccagac gctcgacgag atgatcgagt acgcccgcca
     2221 gatcaaggcg gccggggtgg tcgaccggcc ggtcgtgctc aacatgcagc cctggttcat
     2281 cgagttctgg ctcaccgggg ccggcgccgg cctcgtcggc aacgacaatg gccgcggcga
     2341 gggcgcgacc accgagagcg ccttcgacaa cgaggccacg cgcgacctgt tccagaagct
     2401 gaagaccatg caggacgacg ggctgctcaa cccggtcccg ggcaccgagg gccagttcga
     2461 ccactacttc gccatcggcc tcgagcagtc ttcgatgacc atcgagacct cgaccgcggt
     2521 caccacgatc aactcggtgc tcgagggcaa cctggacccg gccgagctcg gcatcgaggg
     2581 cgagctcccc ccgatcgacg tcaacgtcga cgccggcccg ttcccgggcc tcgaggggcc
     2641 cggtgagggc cagctgggcg gcggggtctg gtacatcacc aacaccacgc cacccgaggt
     2701 ccaggccgcg gcgtgggact tcatcaagta cgtcaacgag cccgagaacc aggcccagtg
     2761 gcacctgcag ggttcgtacc taccggtccg ccaggccacg gtcgattcgg ccgaggtcca
     2821 ggacttctgg gcgaacaccc gcccgggccg ctggctgcag gtcgcctacg agggcctcca
     2881 gaaggtggat ccggaccacc cgggtcccca gatcgggccc tacaccgaga cccgcagcgc
     2941 ggtgcggtcg gctctcgacg cgctcctgtt cggcggcgtc ggcgtggacg gggcgatcac
     3001 ggaggccgac cggacgatca ccgaggcgct ggagagctac gccgacgaga acttctgacc
     3061 accgggatca ctcggcgggg accatccggc gcagcgagcc gggcagctga tcccggcgga
     3121 tctcgccagc ctcggcccgg cgcaggacct cgacgtcgac cccggcgcgg cgggcgaacg
     3181 cgtcgggatc gaggccgctg gcgccgcgga cggcgagcac cgacgccgcc acctccgggt
     3241 ggcggccccc ggaagagccg accaccgacg cgacgcgccg gcggaccgag gccgtgtcga
     3301 ccggggcacg catggagggg tggcgccgac gggcggctgc agccgcgttc atgccaccag
     3361 ctccagctcg gacaggacgg cgaagcgcgg atcggtggcc accacctggc gcaggcgacg
     3421 ccgaccccgg cagatgcgct ggcgcatggc gtcgggcgtg gtgccgtagc gctcggccag
     3481 cgtgtgcgcc ggtgacggat ccccctggac ctgctggatc tggtagtcga gcaggcagac
     3541 gagggcgacg tcgtcgacct tggcggcccg ggccgcctcg gccacgagct ggacccgggg
     3601 atcctcggcc accaggcggg cgaacgcctc ggccaggccc tcctccgccg acggggcggc
     3661 cggttcgacc tcgcggtcgg cccgggacca gtggtcgtcg agcgggtcgg cgtggatgcc
     3721 gacccagcgg ttgacgacgc tgcggatgcg cccctcggcc caccaccagg gcagcgcccc
     3781 accgggccgc cacgcccggg cgaccccgtg cagggccatg cacgcatcga gcacgaggga
     3841 ctgcaggtca tcgggtgcca ccgactcgac accgcacgtg cgcagctggc ggcgcacgac
     3901 gccggcgatg cggtggccgt ggtgctcggc cagggcgaag atggcggccg tgtccccggc
     3961 ggcgaggcgg gccatgatgg cctcgaccgt gaaggggtcg gtgctggtcg gaggggtcgg
     4021 ggaagcgggt gccgggctca tggagaccat catccgcagg gggtctgaca agaacgcggg
     4081 gtggccccgt cacaggccgc cggtacgatg gacgggctgg ccacggccgg cgctgttcat
     4141 tgacaagggg ctgatcggtt tcgacgtcgg gaccggaagc gacatcgtgc gacccgagtt
     4201 gctcagactc gttaatcggg gcacaacatc aactgccaac gcagctgatg ccgaatacgc
     4261 aatggccgcc tgaaccaggt agtcgcgtaa acggcacatt gcacagccga aaggttgtgc
     4321 ggggccagac cgctacagcc tggcaac
//
LOCUS       DATISU010000193         5898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_521798, whole genome shotgun sequence.
ACCESSION   DATISU010000193 DATISU010000000
VERSION     DATISU010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5898
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521798"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VLR27_08035"
     CDS             <1..532
                     /locus_tag="VLR27_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03434.1"
                     /translation="PRAAVAKFAAAGKGIGKKDLGAIARAYGNVYVAQISMGANELQT
                     TKALLEADAWPGPSLVIAYSTCIAHGIDMSKSMSHQKDAVKSGYWPLYRFRPSETEDG
                     QPFTLDSKKPTIPIKDFVSSETRFAILQRTDPERAEELAELAQADADERWRYYEQLVE
                     IQRTVPHAQPHESRED"
     gene            534..1568
                     /locus_tag="VLR27_08040"
     CDS             534..1568
                     /locus_tag="VLR27_08040"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HSP03435.1"
                     /translation="MVDLATTYLGLALRSPIVASASPITDDPDVVRRLEDAGAGAVVL
                     PSLFEEEILHEESELNRALEAGSEHFAEALDYFPTIEELPHAGDRYVGRLERIKAATS
                     LPVIASLNATSPGGWTDHARRLQDAGADALELNLYHLAADPLLAADDVEDRDLEIVAA
                     VREELDIPLAVKLSPYYSAMAGFAQDVVRAGADGLVLFNRFYQPDLDLETLTVVPRVE
                     LSSPSELRLPLRWIAILSPHLAGRASLAATSGVASGADVVKALLVGADVAMTTSALLR
                     HGPEHVAVLESELLAWLTEREYDSVAQLRGSANHSTSGDPAGFERAGYVRTLHSWAAP
                     HHLTPSSPSS"
     gene            1663..2298
                     /locus_tag="VLR27_08045"
     CDS             1663..2298
                     /locus_tag="VLR27_08045"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSP03436.1"
                     /translation="MSIHLGDEAPNFTADTTEGEIDFHEWKKDSWAMLFSHPADFTPV
                     CTTELGRTAAMKDDFAKRNTKVIAISVDDVESHKGWAPDISEVGGTALNFPIIADPDK
                     KVAELYDMIHPGEGDTSTVRSVFIVDPSNKVRLTLTYPKSVGRNFDELLRVVGALQLT
                     DSAPVATPADWTKGEKVIVSPGMSTEDAKERFGADKVEEVKPYLRWTDDPS"
     gene            2340..2873
                     /locus_tag="VLR27_08050"
     CDS             2340..2873
                     /locus_tag="VLR27_08050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HSP03437.1"
                     /translation="MPLLQVIVASTRPGRVGRTVADWFAAEAEAHSGFDVELVDLAEV
                     DLPLFDEPKHPRLQQYEHDHTRRWSETISRGDAFAFVMPEYNFGYNAALKNAIDFLWN
                     EWNDKPVALVGYGGVSGGLRAMTVLKPVLTAVRLRYVTEVPIPFVHDRVEDGRLEANE
                     PMRSGAKGALDALVEAV"
     gene            3000..3533
                     /locus_tag="VLR27_08055"
     CDS             3000..3533
                     /locus_tag="VLR27_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03438.1"
                     /translation="MVTAALFAAVTALRLTGSDEADATGLLYALPVALAAMAFGRVVG
                     TTAALSAAGLLVAWAAGHPEVSLTALGWASRVVPLVLLGVLVGAAADAVEDATDARLA
                     LAVAATRRRDAAEVNDEILQRLTVAKWRLEAGSDADAAALLDEAMSQAQHLVSSLLED
                     VELDDRLRVRTPTGDPG"
     gene            complement(3563..4108)
                     /locus_tag="VLR27_08060"
     CDS             complement(3563..4108)
                     /locus_tag="VLR27_08060"
                     /inference="COORDINATES: protein motif:HMM:NF004168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleosidase"
                     /protein_id="HSP03439.1"
                     /translation="MTGQPLVVVAIGTEAAHLHHLDVVLTGIGKVSAAVAVTRAIAEH
                     RPSFVLNVGTAGALRDGLEGAHRIGRVLEHDVDHAFLRTLTGEDSVGEIELDPGETTT
                     LATGDAFISDPAVRLALGERADLVDMEGFAVARACKAAGVECRMVKVVSDTASEDAAR
                     SWKAEADRTARLIAEVVDQHL"
     gene            complement(4105..4632)
                     /locus_tag="VLR27_08065"
     CDS             complement(4105..4632)
                     /locus_tag="VLR27_08065"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HSP03440.1"
                     /translation="MSASVHLEPHIRDVPDWPEPGVVFKDITPLLADPGGFAYAVRSL
                     AEPFAGRGITKVVGMEARGFLIGAPVALHLGAGFVPVRKPGKLPRATDSEEYALEYGT
                     DRLEIHEDAVGPDDRVLVIDDVIATGGTAAATVRLVERSGATVVGLGFVIELAFLDGA
                     AQLAGHEHVSLLTYR"
     gene            4702..5334
                     /locus_tag="VLR27_08070"
     CDS             4702..5334
                     /locus_tag="VLR27_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HSP03441.1"
                     /translation="MTLTGLEPELERALDLAGVLVFALSGASLAVRRHFDMVGILVLA
                     TVTGLGGGIVRDTLLGQLPPVALRDQLYLAAPLVATAFVLVAHHLVERMHRPVQAFDA
                     AGLGLFCVVGSAKALDAGLGVLPSVLLGTTTAVGGGVIRDVLAREVPTVFKADSALYA
                     IPATLGAAATAAVWSQDALDAPQAGAIAAAVLVVRLLAIRRGWRAPTART"
     gene            5380..>5898
                     /locus_tag="VLR27_08075"
     CDS             5380..>5898
                     /locus_tag="VLR27_08075"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP03442.1"
                     /translation="MDWYLDGSDVTATVALRTEIRDYLERHGDPESDLDSAEVAVSEV
                     LSNLFRHAPGPAWVTLTWATESPLLEVRDLGPGFQPDVSEVPPVDRTGGRGLFIVSAF
                     TKSLETAARAAGGTTVSVVLDVDRPPAESYDPEPSHGPLLPGLDEALPEGGFNRESFL
                     RALVVQLAQAVER"
BASE COUNT          836 a         2225 c         2017 g          820 t
ORIGIN      
        1 gccccgggcc gcggtcgcca agttcgccgc cgccggcaag ggcatcggca agaaggacct
       61 cggcgccatc gcccgggcct acggcaacgt gtacgtggcc cagatctcca tgggcgccaa
      121 cgagctgcag accaccaagg ccctgctcga ggccgatgcc tggcccggcc cgtcgctggt
      181 gatcgcctac agcacctgca tcgcccacgg catcgacatg tcgaagtcga tgagccacca
      241 gaaggacgcg gtgaagagcg gctactggcc cctgtaccgc ttccgcccga gcgagaccga
      301 ggacggccag ccgttcacgc tggactccaa gaagccgacc atccccatca aggacttcgt
      361 ctcctccgag acccgcttcg ccatcctcca gcgcaccgac cccgagcggg ccgaggagct
      421 ggccgagctg gcccaggccg acgccgatga gcgctggcgg tactacgagc agctggtcga
      481 gatccagcgg accgttcccc acgcacaacc gcacgagtcc agggaggact gagatggtcg
      541 acctcgccac cacctacctg gggctcgccc tgcgctcgcc gatcgtggcc tcggcgtcgc
      601 cgatcaccga cgaccccgac gtcgtgcgcc ggttggagga cgccggcgcc ggcgccgtcg
      661 tgctgccgtc gctgttcgag gaggagatcc tccacgagga gtccgagctg aaccgggccc
      721 tcgaggccgg ctcggagcac ttcgccgagg ccctcgacta cttccccacc atcgaggagc
      781 tcccccacgc cggcgaccgc tacgtcgggc gcctggagcg gatcaaggcg gcgacctcgc
      841 tgccggtcat cgccagcctc aacgccacct cccccggtgg gtggaccgac cacgcccgcc
      901 ggctccagga cgccggcgcc gacgcgctcg agctgaacct gtaccacctc gccgccgacc
      961 cgctgctggc ggccgacgac gtggaggacc gcgacctgga gatcgtggcc gccgtgcgcg
     1021 aggagctcga catcccgctc gcggtgaagc tcagcccgta ctactcggcc atggccgggt
     1081 tcgcccagga cgtcgtgcgc gccggggccg acgggctcgt gctgttcaac cgcttctacc
     1141 agccggacct ggacctggag accctcacgg tggtgccccg ggtcgagctg agcagcccgt
     1201 ccgagctgcg cctgccgctg cgctggatcg ccatcctctc cccgcacctg gccggtcggg
     1261 cgtccctggc ggccacctcc ggggtcgcca gcggcgccga cgtggtgaag gccctgctcg
     1321 tcggggccga cgtggccatg accacctcgg cgctgctgcg ccacggcccc gagcacgtgg
     1381 ccgtgctcga gtccgagctg ctggcgtggc tgaccgagcg ggagtacgac tccgtcgccc
     1441 agctccgggg cagcgccaac cactcgacct cgggcgatcc ggccggcttc gagcgcgccg
     1501 gctacgtgcg cacgctgcac tcgtgggccg cgccgcacca cctcaccccg tcctcgccgt
     1561 cgagctgagg gaacaggccg ctcgtcggag gcgttgcctc gggggagccc acgtcctaga
     1621 cctgggtccg tagcaagacg cccccttcga aaggacacgc tgatgagcat ccacctgggt
     1681 gacgaggccc cgaacttcac cgccgacacg acggagggtg agatcgactt ccacgagtgg
     1741 aagaaggaca gctgggcgat gctgttcagc cacccggccg acttcacgcc ggtgtgcacc
     1801 accgagctcg gtcgcaccgc cgccatgaag gacgacttcg ccaagcgcaa caccaaggtc
     1861 atcgcgatct cggtggacga cgtcgagtcc cacaagggtt gggcaccgga catcagcgag
     1921 gtcggcggca ccgcgctgaa cttcccgatc atcgccgacc cggacaagaa ggtcgccgag
     1981 ctctacgaca tgatccaccc cggtgagggc gacacctcca cggtgcgctc ggtgttcatc
     2041 gtcgacccgt cgaacaaggt gcgcctcacg ctgacgtacc cgaagtcggt gggccgcaac
     2101 ttcgacgagc tgctccgcgt cgtcggcgcc ctccagctca ccgactcggc cccggtggcc
     2161 accccggctg actggaccaa gggcgagaag gtcatcgtgt cgcccggcat gtccaccgag
     2221 gacgccaagg agcgcttcgg tgccgacaag gtcgaagagg tcaagcccta cctccgctgg
     2281 accgacgacc ccagctgagt cctcgggtag cgaggggcac gcccgggtag gtcccgggca
     2341 tgcccctcct ccaggtcatc gtcgccagca cccgacccgg ccgcgtcggc cggaccgtcg
     2401 ccgactggtt cgccgccgag gccgaggccc acagcggctt cgacgtcgag ctcgtggacc
     2461 tcgccgaggt cgacctcccg ctcttcgacg agcccaagca cccccggctg cagcagtacg
     2521 agcacgacca cacccggcgc tggtccgaga ccatcagccg cggcgacgcc ttcgccttcg
     2581 tcatgccgga gtacaacttc ggctacaacg ccgcgctgaa gaacgccatc gacttcctct
     2641 ggaacgagtg gaacgacaag ccggtggccc tggtcggcta cggcggcgtg tccggcggcc
     2701 tgcgggccat gaccgtgctc aagccggtgc tgaccgccgt gcggctccgg tacgtgaccg
     2761 aggtgccgat ccccttcgtg cacgaccggg tcgaggacgg ccgcctcgag gccaacgagc
     2821 cgatgcgcag cggcgcgaag ggcgcgctcg acgccctggt cgaggccgtg tgaggcgacg
     2881 tccccggaac ttggtgacat aggaccccgg tgatctcgcc catcgggggc gagagtgtcc
     2941 caatggatcg catcgtcgct ggggggccgt ggttccgacg ccacccgcag tatgcagcaa
     3001 tggtcaccgc cgccctgttc gcggccgtga ccgcgctgcg gctgaccggg tccgacgagg
     3061 ccgacgccac cggcctgctc tacgccctgc ccgtcgccct ggccgccatg gccttcggcc
     3121 gcgtcgtggg caccaccgct gccctgtcgg ccgcgggcct gctggtcgcc tgggcggccg
     3181 ggcatcccga ggtgtcgctg accgcgctcg gctgggcgtc ccgggtcgtg ccgctcgtgc
     3241 tcctcggggt gctggtcggc gccgccgccg atgcggtgga ggatgcgacc gacgcccggc
     3301 tggcgctggc ggtcgccgcg acccgccgtc gcgacgccgc ggaggtcaac gacgagatcc
     3361 tgcagcgcct caccgtcgcc aagtggcgcc tcgaggccgg ctccgatgcc gacgccgccg
     3421 ccctgctgga cgaagccatg tcccaggccc agcacctggt gtcgtcgctg ctcgaggacg
     3481 tggagctcga cgaccggttg cgggtccgca cgccgaccgg cgacccgggc tgacggttct
     3541 ggcagcggct gcggaacaac cgtcagaggt gctggtcgac gacctcggcg atgagacggg
     3601 cggttcggtc ggcttcggcc ttccaggacc gggccgcgtc ctcggacgcg gtgtcggaga
     3661 cgaccttgac catgcggcac tcgacgccgg cggccttgca cgcccgggcg acggcgaagc
     3721 cctccatgtc caccaggtct gcccgctcgc cgagagccag ccgcaccgcc gggtcggaga
     3781 tgaaggcgtc cccggtggcg agggtcgtgg tctcccccgg atcgagctcg atctcaccca
     3841 cgctgtcctc gccggtgagg gtgcgcagga aggcatggtc cacgtcgtgc tccagcaccc
     3901 ggccgatgcg gtgggcgccc tcgagcccgt cgcgcagcgc gccggcggtg ccgacgttga
     3961 gcacgaacga gggccggtgc tcggcgatgg cgcgggtcac ggcgacagcg gccgagacct
     4021 tgccgatgcc ggtgagcacc acgtcgaggt ggtgcaggtg ggcggcttcg gtgccgatcg
     4081 ccacgacgac gaggggctga ccggtcatcg gtaggtgagc aacgacacgt gctcgtgccc
     4141 ggcgagctgg gcggcgccgt cgaggaatgc cagctcgatg acgaagccga ggccgaccac
     4201 cgtcgcgccc gatcgctcca cgaggcgcac cgtggccgcc gcggtcccgc cggtggcgat
     4261 cacgtcgtcg atgacgagca cccggtcgtc gggtccgacg gcgtcctcgt ggatctcgag
     4321 gcggtcggtg ccgtactcca gcgcgtactc ctcggagtcc gtggcccggg gcagcttccc
     4381 cggcttgcgg acgggcacga agccggcgcc gaggtgcagg gccaccggcg cgccgatgag
     4441 gaagccccgg gcctccatcc cgaccacctt ggtgatgcca cggccggcga acggctccgc
     4501 cagggagcgc acggcgtagg cgaagccccc cggatcggcg agcagcgggg tgatgtcctt
     4561 gaagaccacc ccgggctcgg gccagtcggg gacgtctcgg atgtggggct cgaggtgcac
     4621 cgatgcggac accggaggca atgtacggga cccgcacgat cccgaacgac ggagcctgcc
     4681 gatgccgaag gccgcccccc ggtgacgctc accggcctcg agcccgagct ggagcgcgcc
     4741 ctggacctcg ccggcgtgct ggtcttcgcc ctgtctggcg cctcgctggc ggtgcgccgc
     4801 cacttcgaca tggtcggcat cctggtcctc gccaccgtca ccggccttgg cggcggcatc
     4861 gtgcgcgaca ccctcctcgg ccagctcccg ccggtcgccc tgcgcgacca gctctacctg
     4921 gccgcccccc tggtggcgac ggccttcgtg ctcgtcgccc accacctcgt ggagcgcatg
     4981 caccgccccg tgcaggcctt cgacgcagcc gggctcgggc tgttctgcgt ggtcggctcg
     5041 gccaaggcgc tcgacgccgg actcggcgtg ctcccctcgg tcctgctcgg caccacgact
     5101 gccgtcggcg gcggcgtgat ccgcgacgtc ctggcccggg aggtccccac ggtcttcaag
     5161 gccgacagcg cgctctacgc catcccggcg acgctgggcg ccgccgccac cgcagcggtc
     5221 tggtcccagg acgccctcga cgcaccacag gcgggcgcca tcgccgccgc cgtcctggtg
     5281 gtgcgcctgc tcgccatccg ccgcggctgg cgagcgccga ccgcacggac gtagcaccga
     5341 acccgcccac gtccggttcg tggcggtatc gtccactact tggactggta cctcgacggt
     5401 tcggacgtca ccgccaccgt cgccctccga acagagatcc gcgactacct ggagcgccac
     5461 ggcgatccgg agtccgatct ggactccgca gaggtcgcgg tcagcgaggt gctgtcgaac
     5521 ctgttccgcc acgcaccggg tccggcgtgg gtcaccctca cctgggccac ggagtcgccg
     5581 ctgctcgagg tgcgtgacct cggccccggc ttccagcccg acgtgtccga ggtcccgccg
     5641 gtcgatcgca ccggtggtcg tgggctgttc atcgtcagcg cgttcaccaa gtcgctcgag
     5701 accgcggcgc gggcggccgg ggggaccacc gtcagcgtcg tgctcgacgt cgaccgtcct
     5761 cccgccgaga gctacgaccc cgaaccctcc cacggcccgc tgctgcccgg cctcgacgag
     5821 gccctgcccg aaggcgggtt caaccgcgag tcgttcctgc gtgcgctcgt ggtgcagctc
     5881 gcccaggccg tcgagcgc
//
LOCUS       DATISU010000194         6900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_475330, whole genome shotgun sequence.
ACCESSION   DATISU010000194 DATISU010000000
VERSION     DATISU010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475330"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VLR27_08080"
     CDS             complement(<1..537)
                     /locus_tag="VLR27_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSP03443.1"
                     /translation="MADVPPTSDPTPAAPDARAAVIAVRDEVAKVVVGQDGVLSGIIA
                     ALLVGGHVLLEGVPGVAKTLLVKTVAAAFDLDFKRVQFTPDLMPSDVLGQVIYDAEQG
                     FRFREGPAFTNVLLADEINRTPPKTQAALLEAMEERQVSVEGAARRLPEPFAVIATQN
                     PVEYEGTYPLPEAQLDRFL"
     gene            complement(512..1606)
                     /locus_tag="VLR27_08085"
     CDS             complement(512..1606)
                     /locus_tag="VLR27_08085"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HSP03444.1"
                     /translation="MSRRIAFGALLVAAVLVGAYLTAARQTQGSPLDPSSTAPDGARA
                     VVELVDALATMDVLDEIPGDDVDAALVLQDRFDRDAQDALLDWVDAGGTLVVADVDST
                     TTPPVVGTVTGPFEVTCAGDVPAGLADVSRLEVADGFRVAVPAGAAGSCSPPGDGALV
                     VVEDVGAGRVVSVAGPSTFTNRYLDEADNAVLAAALLAPDAGTRAAFLSPSLVVGTGD
                     AGLVDLVDTPVRAALAQLVVAFLLAALWRARRLGRPVAEPQPVPIEASELTQAVGRVL
                     ARSDRPGPAAAALRDRARRDLSAPLGLPLDASAEAVVDALSRRTELSPAEARRAAIDP
                     VTTDDDLVEVATLLTRIRKDTTHGRRPTHV"
     gene            complement(1603..2253)
                     /locus_tag="VLR27_08090"
     CDS             complement(1603..2253)
                     /locus_tag="VLR27_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HSP03445.1"
                     /translation="MRSWQGEPGGLVDPDEVRRTAEEILSRPEYREPQPSLLDRALEA
                     ITDFLGRSFAALTGGGAGSIIGLVITALVLALALWLLAKALRTSWSRSPGSDAVGVVQ
                     GTSAPDDPAVWDDEADRLTAAGDHRGALRCRYQALVASLVRRGAVDDVAARTPAELRR
                     ELAGRQPDLDPVLDSVTERFEAAWYGGRPVDAGALTSFRADVDALRAADLRPVVRA"
     gene            complement(2234..3163)
                     /locus_tag="VLR27_08095"
     CDS             complement(2234..3163)
                     /locus_tag="VLR27_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03446.1"
                     /translation="MTEATWAAPDSAPAQPERPLATEPPASGPRRDRDRPPLPPLPVA
                     LRPMTTPDLLDGAFAIIKRRPRDVLTLAAVLLIPIEVLSAVLLRDVLDADGLTGLADP
                     TTAFGEDGEVVGAGAALTSLAVGTISLALLAGALGVLVDGWYRGRDVSAWEAAGVALR
                     RSWALVLGTVLVHVLEGIGLLAFGVGAYLVMALCHVVSPVITVESLGPLAAIRRSVQL
                     TRRRIGPALSVPGLVGLVGLLIGFGFQSVPELATTVVPDDWDWLVRAAGQITSQLVVV
                     PFTAGVAVLFHLDLRIRLEAHDIELRSRALLAG"
     gene            complement(3160..4203)
                     /locus_tag="VLR27_08100"
     CDS             complement(3160..4203)
                     /locus_tag="VLR27_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03447.1"
                     /translation="MSDDRRRPGQHESRRDEALRRWIAEEVHPFSEPVRAQLERSGLG
                     RRGVRVAGDLARLPVTELGDLGDGRGHVLEPTAERIQESAGPGLRVRLMAADVLGRRE
                     GFARHDVDLPYKPVRWTVERHVAGPLFVAATNTDLDRLAALGRRALAVSGITPADRLL
                     CTGAGIGPLQLELGARDAGVAHLVVESPLAASVLADAAPTVVAGPPATLSRLVDAGLP
                     ASVRLLVAHVGAAGGEDLVALRRRAGRPVRLWWAPPGVRAAWATCEADQLHTWPEHEH
                     LEVVDDEGLPAESGRLVWSAVGWRGSVWLRVALGPEGRVDRSACSCGRTTPRVRGAAR
                     VRRTPEPAGGSRR"
     gene            4245..5024
                     /locus_tag="VLR27_08105"
     CDS             4245..5024
                     /locus_tag="VLR27_08105"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP03448.1"
                     /translation="MVPTAGDEDESGMRGYGPATYGDGFADVYDDWYADVSPPEATAR
                     FLADRTRGPVLELGSGTGRLAAPLRTAGVPVIGLDASVAMLTRSVDRHPHVPVVAADM
                     AELPVRDGGFGAVLVAFNTLFNLPTLALQRRCLTQARRALRPDGIVVVEAFVPGAGAD
                     ERTDHVDVVRLDADVVVLRVSRTDPERGTVHGHHVELRDGQPVRLRPWQVCFATPDAL
                     DDMAAAAGLERVERHGGWAGEGFDAASDAHVTVYRVAQGGG"
     gene            5035..6027
                     /gene="galT"
                     /locus_tag="VLR27_08110"
     CDS             5035..6027
                     /gene="galT"
                     /locus_tag="VLR27_08110"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HSP03449.1"
                     /translation="MSELRLNPLTGRWVTIASTRQDRPGELVSRELPIEADPDQPCPF
                     CPGNEEETPPSLEEYGSSGAWSVRVVPNKYPAFSGSEPMRVTNLGPVFTQANASGIHE
                     VLVLSPEHDQSWGDLDDKQAGLVMAAIRDRMEDHGRQAGTRYTQAIVNAGRAAGASLE
                     HPHGQLLGIPFVPGEIAEEQRGFDRFDGSCLLCTAVQAELEAGHRVVHADERVVVLCP
                     FWSASPYQMLVVPRTHAEHLTDATPKDLVAVGRAIRDSLMALRAVMGHVSYNLVFHTA
                     PHHHPGDFHWHVHVNPRITSVAGFEQGTGVMINIMPPELACEHLRANAPLSASA"
     gene            6034..>6900
                     /locus_tag="VLR27_08115"
     CDS             6034..>6900
                     /locus_tag="VLR27_08115"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSP03450.1"
                     /translation="MSTPDAADTARTWPFWAPSQAEAVEAALDLADVGPGVHLLDLGC
                     GDGQVLLAAARRGATVSGVEADADLVAEAQDHLAEAGVDADVRQGDLFDATVDVGADV
                     LFSYLAPATLQRLLPRIVETGARLVTVDFDVPGLVPAKRSGAARLYRLPGRRRTIGEP
                     GWPTAGTLVATVPDVQSLSCLEAAHAGGPTLVRADAGLASVATVFSGADHLDEAGALA
                     VDLRWEGKPAGTFAGGIIHVEGLEDHHVFVLHTDEEEAVWELSADGVAGLHQALLATH
                     RPSTIDEALAAAN"
BASE COUNT          899 a         2593 c         2631 g          777 t
ORIGIN      
        1 caggaagcgg tcgagctggg cctcgggcag cgggtaggtg ccctcgtact ccaccgggtt
       61 ctgggtggcg atcaccgcga acggctcggg cagccggcgg gccgcgccct cgaccgacac
      121 ctgccgctcc tccatcgcct cgagcagcgc ggcctgggtc ttgggcggcg tgcggttgat
      181 ctcgtcggcg agcagcacgt tggtgaaggc cggtccctcc cggaaccgga agccctgctc
      241 ggcgtcgtag atgacctggc ccaacacgtc ggacggcatc aggtccgggg tgaactgcac
      301 ccgcttgaag tcgaggtcga aggccgcggc caccgtcttc accagcaggg tcttggccac
      361 gccgggcacg ccctcgagca gcacatggcc gccgacgagg agcgcagcga tgatgcccga
      421 gagcacaccg tcctggccga cgacgacctt ggccacctcg tcgcgcacgg cgatcacggc
      481 ggctcgggcg tcgggggcgg cgggggtggg gtcagacgtg ggtgggacgt cggccatggg
      541 tggtgtcctt ccggatgcgg gtgagcaggg tcgccacctc gacgaggtcg tcgtcggtgg
      601 tgacggggtc gatggcggcg cggcgggcct cggccgggct cagctcggtg cgccggctga
      661 gggcgtcgac caccgcctcg gcggaggcgt cgaggggcag gccgaggggg gcggagaggt
      721 cgcgccgggc ccggtcgcgg agcgccgcgg ccgccgggcc gggacggtcg ctgcgggcga
      781 gcacgcgccc gacggcctgg gtgagctcgc tggcctcgat gggcaccggc tgcggctccg
      841 ccaccgggcg gccgaggcgg cgggcccgcc agagggcggc gagcaggaac gccacgacga
      901 gctgggcgag cgcggcccgc accggcgtgt cgaccaggtc gaccaagccc gcgtcgccgg
      961 tgccgacgac gagcgacggg ctcaggaagg cggcccgggt gccggcgtcg ggcgcgagga
     1021 gcgcggcggc gaggacggcg ttgtcggcct cgtccaggta ccggttggtg aaggtgctcg
     1081 gcccggcgac gctcacgacg cgccccgcgc ccacgtcctc cacgacgacg agggcgccgt
     1141 cgccgggcgg gctgcagctg ccggcggcgc cggccgggac ggcgacgcgg aagccgtcgg
     1201 ccacctcgag ccgggaaacg tcggcgaggc ccgcggggac gtcgccggca caggtcacct
     1261 cgaacggacc ggtcaccgtg cccaccaccg gtggcgtggt ggtggagtcg acgtcggcga
     1321 ccacgagggt gccgccggcg tccacccagt cgagcagcgc gtcctgggcg tcgcggtcga
     1381 agcggtcctg caggaccagg gcggcgtcga cgtcgtcgcc ggggatctcg tcgagcacgt
     1441 ccatcgtggc cagcgcatcg accagctcga ccaccgcccg ggccccgtcc ggcgccgtgg
     1501 acgacggatc gaggggcgac ccctgggtct ggcgggccgc ggtgaggtag gcgccgacga
     1561 ggaccgccgc caccagcagg gcgccgaagg cgatgcggcg gctcacgccc gcaccaccgg
     1621 tcgcaggtcg gcggcgcgca gggcgtcgac gtcggctcgg aaggaggtga gcgcgccggc
     1681 gtccacgggc cgcccgccgt accaggcggc ttcgaagcgc tccgtcaccg agtcgagcac
     1741 cgggtcgagg tccggttgcc gtccggccag ctcgcgccgc agctcggccg gggtccgggc
     1801 cgcgacgtcg tccacggcgc cgcggcgcac gaggctggcg acgagggcct ggtaccggca
     1861 gcgcagcgca ccccggtggt cgccggcggc ggtgaggcgg tcggcctcgt cgtcccacac
     1921 cgccgggtcg tcgggggccg aggtgccctg caccacgccg accgcgtcgc tgccgggcga
     1981 tcgggaccag gacgtgcgca gcgccttggc gagcagccag agggccagcg ccagcaccag
     2041 ggcggtgatg accagaccga tgatgctgcc cgcaccacca ccggtgaggg cggcgaagga
     2101 ccggccgagg aagtcggtga tcgcctccag cgcccggtcg agcagcgagg gctgcggctc
     2161 ccggtactcg ggtcgggaca ggatctcctc ggccgtgcgg cgcacctcgt cggggtcgac
     2221 gaggccgccc ggctcaccct gccaggagcg cacgggagcg cagctcgatg tcgtgggcct
     2281 cgagccggat gcgcaggtcg aggtggaaca ggacggcgac gccggcggtg aacggcacga
     2341 ccaccagctg ggacgtgatc tggccggcgg cgcgcacgag ccagtcccag tcgtcgggca
     2401 cgaccgtggt ggccagctcg gggaccgact ggaacccgaa gccgatgagg aggccgacca
     2461 ggccgaccag gccggggacg gagagcgccg gaccgatccg acgccgggtc agctgcaccg
     2521 agcgccggat ggcggccagc ggtccgagcg actcgaccgt gatcaccggg ctcaccacgt
     2581 ggcacagcgc catgaccagg tacgcgccga cgccgaaggc caggaggccg atgccctcga
     2641 gcacgtggac caggaccgtg ccgagcacca gggcccagct gcggcgcagc gccactcccg
     2701 ccgcctccca ggccgacacg tcgcggcccc ggtaccagcc gtcgaccagc acgccgagtg
     2761 ctccggcgag cagggccagc gagatcgtcc ccacggcgag ggaggtgagg gcggcaccgg
     2821 cgccgaccac ctcgccgtcc tcaccgaacg ccgtggtcgg gtcggcgagg ccggtgagcc
     2881 cgtcggcgtc gagcacgtcg cggagcagca cggcgctgag cacctcgatg gggatgagca
     2941 gcacggcagc cagggtcagc acgtcccgcg gacggcgctt gatgatggcg aaggcgccgt
     3001 cgagcaggtc cggggtggtc atcggccgca gggccaccgg caggggcggc agcggcggac
     3061 ggtcgcggtc gcgccgggga ccgctggcgg gcggctcggt ggcgagcgga cgctcgggct
     3121 gcgccggcgc gctgtcgggc gcggcccagg tcgcctccgt catcggcgcg acccgccggc
     3181 cggctccggg gtgcgtcgga cgcgagccgc gccgcgcacc cgcggcgtgg tccgcccgca
     3241 cgagcacgcg ctgcggtcga cccggccctc gggaccgagc gccacccgca gccacaccga
     3301 cccgcgccac ccgacggccg accacaccag ccggccggac tcggcgggga gcccctcgtc
     3361 gtcgacgacc tcgaggtgct cgtgctcggg ccaggtgtgc agctggtcgg cctcgcaggt
     3421 cgcccacgcc gcccggaccc ccggcggcgc ccaccacagc cggaccggcc gtccggcccg
     3481 ccggcgcaag gccacgaggt cctcgccacc tgcggccccc acgtgggcga ccaggagccg
     3541 cacgctggcc gggagcccgg cgtcgacgag ccgggacagc gtggcgggcg ggccggcgac
     3601 gacagtgggt gcggcatcgg cgaggacgct ggccgccagc ggggactcga ccaccaggtg
     3661 ggccacgccg gcgtcgcggg cgccgagctc gagctgcagc ggcccgatcc cggcgccggt
     3721 gcagaggagg cggtcggcgg gcgtgatgcc gctgacggcc agcgcccgcc ggccgagggc
     3781 ggcgagccgg tcgaggtcgg tgttggtggc cgccacgaag agcgggccgg cgacgtggcg
     3841 ttcgacggtc cagcgcaccg gcttgtacgg caggtccacg tcgtggcggg cgaacccctc
     3901 ccggcggccg aggacgtcgg cggccatgag ccggacccga agccccggac cggccgactc
     3961 ctggatgcgc tcggccgtcg gttcgagcac gtggccacga ccgtcaccga ggtcaccgag
     4021 ctcggtgacc ggcagccggg ccaggtcgcc ggcgacccgc acgccccgac gtccgaggcc
     4081 gctgcgctcg agctgggccc gcaccggctc ggagaacggg tggacctcct cggcgatcca
     4141 ccggcggagc gcctcgtccc ggcgcgactc gtgttgcccg ggccgccggc ggtcgtcgct
     4201 catcacggcg cacgctactg aggcgtgcgc ggcaagatgc ccgcatggtg ccgaccgccg
     4261 gagacgagga cgagtccggc atgcgcggct acgggccggc cacctacggg gacggcttcg
     4321 ccgacgtcta cgacgactgg tacgccgacg tctccccgcc cgaggcgacc gcccggttcc
     4381 tggccgaccg cacgaggggt ccggtgctgg agctgggcag cggcaccggc cgcctggccg
     4441 cgccgctgcg gacggccggc gtgccggtca tcggcctgga tgcgtcggtg gccatgctca
     4501 cccgctccgt cgaccgccac ccccacgtcc ccgtcgtcgc cgccgacatg gccgagctgc
     4561 cggtgcggga cggcgggttc ggggcggtgc tggtcgcgtt caacaccctc ttcaacctcc
     4621 cgaccctcgc gctgcagcga cgctgcctca cccaggcccg ccgggccctc cgccccgatg
     4681 gcatcgtcgt ggtcgaggcg ttcgtgcccg gggccggggc cgacgagcgg accgaccacg
     4741 tcgatgtggt ccggctcgat gccgacgttg tcgtgctgcg ggtgagccgc accgacccgg
     4801 agcggggcac ggtgcacggc caccacgtgg agctgcgcga cggccagccg gtgcggctgc
     4861 ggccgtggca ggtgtgcttc gcgacacccg acgcgctcga cgacatggct gctgccgccg
     4921 ggctcgaacg cgtcgagcgt cacggcgggt gggcaggtga gggcttcgat gccgccagcg
     4981 acgcccacgt cacggtgtac cgggtcgccc agggcggtgg gtagtgtccg ggcagtgagc
     5041 gagctccgcc tgaaccccct caccggtcgc tgggtcacca tcgcaagcac ccgacaggac
     5101 cgtccgggcg agctggtgtc gagggagctc ccgatcgagg ccgaccccga tcagccgtgc
     5161 ccgttctgcc ccggcaacga ggaggagacg ccgccgtcgc tcgaggagta cggcagcagc
     5221 ggcgcgtggt cggtgcgggt ggtgccgaac aagtacccgg ccttctccgg cagcgagccc
     5281 atgcgggtca ccaacctcgg cccggtcttc acccaggcga acgcctcggg catccacgag
     5341 gtgctggtgc tctcccccga gcacgaccag tcgtggggcg acctcgacga caagcaggcc
     5401 ggcctcgtca tggccgccat ccgcgaccgg atggaggacc acggccgcca ggcgggcacc
     5461 cgctacacgc aggccatcgt caacgcgggc cgggctgccg gcgcctcgct cgagcacccc
     5521 cacggccagc tgctcggcat ccccttcgtc cccggcgaga tcgccgagga gcagcgtggc
     5581 ttcgaccgct tcgacgggtc ctgcctgctg tgcaccgccg tccaggccga gctcgaggcc
     5641 ggccaccggg tcgtccacgc cgacgagcgg gtcgtggtgc tctgcccctt ctggagcgcg
     5701 tccccctacc agatgctggt ggtgccccgg acccacgccg agcacctcac cgacgccacg
     5761 ccgaaggacc tcgtcgcggt cggccgcgcc atccgcgact cgctgatggc cctgcgcgcc
     5821 gtcatgggcc acgtgtcgta caacctcgtg ttccacaccg cgccgcacca ccacccgggc
     5881 gacttccact ggcacgtgca cgtgaacccg cggatcacct cggtggccgg cttcgagcag
     5941 ggcaccggcg tgatgatcaa catcatgccg ccggagctgg cctgcgagca cctgcgcgcc
     6001 aacgccccgc tcagcgccag cgcctgaccc acggtgtcga ccccggacgc ggcggacacc
     6061 gcccgcacct ggcccttctg ggcacccagc caggccgagg cggtggaggc cgcgctcgac
     6121 ctcgccgacg tcggtccggg cgtccacctg ctggacctgg gctgcggcga cgggcaggtc
     6181 ctgctggccg ccgcccgccg tggcgccacc gtgtccggcg tggaggccga cgccgacctc
     6241 gtcgccgagg cgcaggacca cctggccgag gcgggcgtgg acgccgacgt ccgccagggc
     6301 gacctgttcg acgccaccgt cgacgtgggc gccgacgtgc tgttctcgta cctggccccc
     6361 gcgaccctgc agcggctcct gccccggatc gtcgagaccg gggcgcgcct ggtgacggtg
     6421 gacttcgacg tgcccggcct ggtgccggcc aagcgcagcg gcgccgcccg cctctaccgg
     6481 ctgcccggcc gccggcgcac catcggcgaa ccgggctggc ccaccgccgg caccctggtc
     6541 gccaccgtgc ccgacgtgca gtcgctcagc tgcctcgagg ccgcccacgc cggcggtccg
     6601 acgctggtgc gcgccgatgc cggcctggcg tcggtggcca ccgtcttctc cggcgccgac
     6661 catctcgacg aggcgggcgc gctggccgtc gacctgcgct gggagggcaa gccggccggc
     6721 accttcgccg gcgggatcat ccacgtcgag ggcctcgagg accaccacgt cttcgtgctc
     6781 cacaccgacg aggaggaggc ggtctgggag ctcagcgccg acggcgtcgc cggcctccac
     6841 caggcgctcc tcgccaccca ccgcccgtcg acgatcgacg aggccctcgc cgccgccaac
//
LOCUS       DATISU010000195         4108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_335260, whole genome shotgun sequence.
ACCESSION   DATISU010000195 DATISU010000000
VERSION     DATISU010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335260"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2927
                     /locus_tag="VLR27_08120"
     CDS             <1..2927
                     /locus_tag="VLR27_08120"
                     /inference="COORDINATES: protein motif:HMM:NF023275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase family
                     protein"
                     /protein_id="HSP03451.1"
                     /translation="YRWGNTVDPVLPGLMDRPYAARELIPYGTPPSADLLNALDLELQ
                     EQTMSDQGLAAVARLMGVGDVVLRNDTQYERYNTPRPRSLLDLVSSMPGFGDPITFGD
                     DVPNVPIEDAPMIDEQHLARDGALPESPAIVAFPVEDARRIVRTHAAEQVLLLSGSGA
                     GIVDAAQAGLIEGDELIRYSAELTEDPDFVRDHLRADRGLIVTDTNRKRGERWTTVRH
                     TQGFTETEEGGILGTDRTDNRLPVLPERPGTRTVAEHRGVEVQASDYGNPITYTPEER
                     PANGVDGDPTTAWRVAAFADAVGHRIVLTAPRPVTTDEVTLLQPQTGEINRWITEVEL
                     SFDGDDQMTVELDESSRAQPGQTIDIGERTFRELTIEIRADTAGRRPGWDNLTSVGFA
                     EIGVAGLEVDEVIRMPSDLLDAGGFRTLSYPLALTQTRVRSIATEVTRTDEEFSMARA
                     VDLPTTRDYALGGTARLSARAPSPVLDALLGRGGLEADQPRVTATSSLPGGLRHLPSN
                     VLDGDDATWWTTTFAAPPGQTLRIESPEPATISEVSLLLVDDEEHSVPTAVQVLADGN
                     EVVAAPLSTAPAGDGLVSATVALPAPVTASVIEVAFPEIEPRTTMDWYSKGPVVLPIA
                     VAEAGVGGLTVPGLADTVDSGCRDDLVEVDGRPVEVRVRGSVADALDGAGLAISTCAG
                     GVTVAGGDRTFRTAEGRVTGIDIDQLVWRSDAGGIAPTSATAIPLMDDAPDAPRVRVL
                     SEDDATVQVQVDGADPGSPFWLVLGQSHSPGWAFESADAEADGTRLVDGYANGFLVTP
                     TSSTFEATLRFTPQNRVEVGLLTSAIAVLGVIVLALLPVREIRPLPIPLQEPLRRLRA
                     LSWEGALPTRRDARVLGVVVAIATALLVTVPIGVAVGLAAGFATRRETWRPIFTLLPA
                     GLLAVAAGYVMVLQLRNEIPPGLHWPNQTGRLHPLGLTAVVLLGVDVAIAQLWARRSD
                     YR"
     gene            2959..3630
                     /locus_tag="VLR27_08125"
     CDS             2959..3630
                     /locus_tag="VLR27_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP03452.1"
                     /translation="MSAPVVLVHGFGTSFELTWVHNGWVDLLADEGREVIGVDLLGHG
                     DAPKPTDPEAYSDLGERVRDALPDEPVDAIGFSLGAKTLLQVAAADPSRFRSLIVSGV
                     GSNLFQRSDHTAVVEAVRTGDDGGNPALRYFAGLADLPGNDREALAACMSNGGGGVTP
                     EQLAAITCPVLVVLGDQDFTGPADPLVEALPDATYVELARTDHAKTPKSFAFIDAALE
                     FLRAR"
     gene            3627..>4108
                     /locus_tag="VLR27_08130"
     CDS             3627..>4108
                     /locus_tag="VLR27_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03453.1"
                     /translation="MIFGVDLDGVCGDFSSAFAAVVADDLGVAVEDLPPRQHWDFSEW
                     GIRDEAHFRSVHERAVLEHRVFRTMPAIAGCADALWRLSDAGVWIRLITHRLYVNWGH
                     QEVVGDTVAWLDEHGIPYRDLCFLGQKPEVQADIYVDDAPHNVEALRAAGNDVITFAQ
                     P"
BASE COUNT          553 a         1641 c         1371 g          543 t
ORIGIN      
        1 cctaccgctg gggcaacacc gtcgaccccg tcctccccgg cctgatggac cgcccgtacg
       61 cggcccggga gctcatcccc tacggcacgc ccccctcggc ggacctgctg aacgccctgg
      121 acctggagct gcaggagcag accatgtcgg accagggcct ggcggcggtg gcgcggctca
      181 tgggcgtcgg cgacgtcgtg ctgcgcaacg acacccagta cgagcgctac aacacccccc
      241 gaccccggtc cctgctcgac ctcgtgtcgt cgatgcccgg cttcggcgac ccgatcacgt
      301 tcggcgacga cgtccccaac gtcccgatcg aggacgcacc gatgatcgac gagcagcacc
      361 tggcacgcga cggcgcgctg cccgagtcgc ccgccatcgt ggcgttcccg gtggaggacg
      421 cccgtcggat cgtgcgcacg cacgccgccg agcaggtgct gctcctcagc ggctccggcg
      481 ccggcatcgt cgacgcggcc caagccggcc tcatcgaggg tgacgagctg atccgctact
      541 ccgccgagct caccgaggac ccggacttcg tccgagacca cctgcgcgcc gaccgggggc
      601 tgatcgtcac cgacaccaac cgcaagcgcg gcgagcgctg gaccacggtg cgccacacgc
      661 agggcttcac cgagaccgag gagggcggca tcctcggcac cgaccgcacc gacaaccgcc
      721 tccccgtcct gcccgagcga cccggcacgc ggaccgtcgc cgagcaccgc ggtgtggagg
      781 tccaggcatc ggactacggg aacccgatca cctacacacc cgaggagcgt ccggccaacg
      841 gggtcgacgg cgaccccacg acggcctggc gcgtggccgc cttcgccgac gctgtcggcc
      901 accgcatcgt cctcaccgcc ccgcgaccgg tgaccaccga cgaggtcacc ctgctgcagc
      961 cccagaccgg ggagatcaac cgctggatca ccgaggtcga gctgtccttc gacggcgacg
     1021 accagatgac cgtcgagctg gacgagtcct cccgggccca gccggggcag accatcgaca
     1081 tcggcgagcg gaccttccgg gagctgacca tcgagatccg ggccgacacg gccggacgtc
     1141 ggcccggctg ggacaacctc accagcgtcg ggttcgccga gatcggggtc gccggcctcg
     1201 aggtcgacga ggtcatccgc atgccgagcg acctgctgga cgccggcggg ttccgaacgc
     1261 tgtcctaccc gctcgccctc acccagacga gggtgcgctc gatcgccacc gaggtcaccc
     1321 gcaccgacga ggagttctcg atggcccgcg ccgtcgacct ccccacgacc cgcgactacg
     1381 ccctcggcgg gaccgctcgc ctgtccgccc gcgcgccgtc accggtcctc gacgccctgc
     1441 tcggccgggg tggcctcgag gccgaccaac cccgggtcac cgccaccagc tcgctccccg
     1501 gcggcctgcg ccaccttccc tccaacgtcc tcgacggcga tgacgccacg tggtggacca
     1561 ccaccttcgc cgcgccgccg ggccagacgc tgcgcatcga gtcccccgag ccggccacga
     1621 tctccgaggt ctccctgctg ctggtggacg acgaggagca ctcggtgccg accgccgtgc
     1681 aggtcctcgc cgacggcaac gaggtcgtgg ctgcgccgct gtcgaccgca ccggccggcg
     1741 acggtctcgt gtccgccacg gtcgcactgc cggctccggt cacggcgagc gtcatcgagg
     1801 tcgcgttccc cgagatcgaa ccccgcacga cgatggactg gtacagcaag ggcccggtcg
     1861 tgctccccat cgccgtcgcc gaagcgggcg tcggcgggct caccgtgccc ggcctcgccg
     1921 acacggtgga cagcgggtgc cgggacgacc tcgtcgaggt cgatggccgc cccgtcgagg
     1981 tgcgcgtccg cggcagcgtc gccgacgcgc tcgacggcgc cggccttgcg atctcgacct
     2041 gcgccggggg cgtgaccgtg gccggcggcg accgcacgtt ccggaccgcc gagggacggg
     2101 tcaccggcat cgacatcgac cagctggtgt ggcggtccga cgccggcggc atcgcgccca
     2161 ccagcgccac cgccatcccg ctcatggacg acgcgccgga cgcgccccgg gtccgggtcc
     2221 tctccgagga cgacgcgacc gtccaggtcc aggtcgacgg cgccgacccc ggatcgccgt
     2281 tctggctggt cctcgggcag agccacagcc cgggctgggc gttcgagtcc gcggacgccg
     2341 aggccgacgg cacccggctg gtggacggct acgcgaacgg gttcctggtg acgcccacgt
     2401 cgtcgacgtt cgaggcgacc ctgcggttca ccccgcagaa ccgggtcgag gtcggcctcc
     2461 tcaccagcgc catcgccgtc ctcggcgtca tcgtgctcgc cctgctcccg gtccgggaga
     2521 tccgcccgct gccgatcccc ctgcaggagc cgctccggcg cctccgggcc ctgagctggg
     2581 agggggcgct gcccacgcgc cgcgacgcac gggtgctcgg ggtcgtcgtc gccatcgcca
     2641 ccgccctgct cgtcaccgtg ccgatcggtg tggccgtggg gttggccgcc ggcttcgcca
     2701 cgcgccgcga gacctggcgg ccgatcttca cgctcctgcc cgccgggctg ctcgccgtgg
     2761 ccgccggcta cgtcatggtg ctgcagctgc gcaacgagat cccgccgggc ctgcactggc
     2821 ccaaccagac cggacggctc caccccctcg ggctcaccgc cgtcgtgctg ctcggcgtcg
     2881 acgtggccat cgcccagctg tgggcccgcc gcagcgacta ccgctgacgc cgtccgctcc
     2941 gctggcagac tgccgcccat gagcgctccg gtggtcctgg tccacggctt cggcacgtcc
     3001 ttcgagctga cgtgggtgca caacgggtgg gtcgacctgc tcgccgacga gggccgggag
     3061 gtcatcggcg tcgacctcct cggccacggg gatgccccca agcccaccga ccccgaggcc
     3121 tacagcgacc tcggcgagcg ggtgcgcgac gcgctgcccg atgagccggt cgacgccatc
     3181 gggttctccc tcggggccaa gacgctgctc caggtggccg ccgccgaccc gtcccggttc
     3241 cggtcgctga tcgtctccgg cgtcgggtcc aacctgttcc agcgcagcga ccacaccgcc
     3301 gtcgtggagg cggtgcgcac cggtgacgac ggtgggaacc cggcgctgcg ctacttcgcc
     3361 ggcctcgccg acctgcccgg caacgaccgg gaggcgctgg ccgcctgcat gagcaacggc
     3421 ggcggcgggg tcacccccga gcagctggcg gccatcacct gccccgtgct cgtcgtgctc
     3481 ggcgaccagg acttcaccgg accggccgac ccgctcgtcg aggcccttcc cgacgccacc
     3541 tacgtcgagc tggcccgcac cgaccacgcc aagacgccca agtccttcgc gttcatcgac
     3601 gccgccctcg agttcctccg cgcccggtga tcttcggcgt cgacctcgac ggcgtctgcg
     3661 gcgacttctc ctcggccttc gccgcggtcg tcgccgacga cctcggcgtg gcggtggagg
     3721 acctgccgcc ccgccagcac tgggacttct ccgagtgggg catccgcgac gaggcccact
     3781 tccgcagcgt ccacgagcga gcggtgctgg agcaccgcgt gttccgcacg atgcccgcca
     3841 tcgcgggctg cgccgatgcg ctgtggcggc tctcggacgc cggcgtgtgg atccgcctca
     3901 tcacccaccg cctgtacgtg aactggggcc accaggaggt ggtcggcgac acggtggcgt
     3961 ggctggacga gcacggcatc ccctaccgcg acctgtgctt cctcggccag aagcccgagg
     4021 tgcaggccga catctacgtc gacgacgccc cccacaacgt cgaggcgctc cgggccgccg
     4081 gcaacgacgt catcaccttc gcccagcc
//
LOCUS       DATISU010000196         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_195855, whole genome shotgun sequence.
ACCESSION   DATISU010000196 DATISU010000000
VERSION     DATISU010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195855"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VLR27_08135"
     CDS             <1..864
                     /locus_tag="VLR27_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose synthase"
                     /protein_id="HSP03454.1"
                     /translation="FDFPLMQAMYLALARCDAGPIRGALEARPEIPEDAQWAIFVRNH
                     DELTLDQLSDDERQEVFDAFGPEEHMQLYGRGLRRRLPPMLDGDQDRIRMVYSLLFSL
                     PGTPVLFYGEEIGMGENLDIEGRLSVRTPMQWTAGKNAGFSSARPSRLARPVPDGQFG
                     PLAINVADQRRQSDSLLTWMERMIRRRRETPELGWGATMLLDTGDDAVLAHRCDWERS
                     AVVAIHNLSPTPRELRLDLGPMEGCDQVVDLLDAAQPVHEVDGSTIDLTLAGYAHHWF
                     RLQDSSGSTPP"
     gene            922..>2495
                     /locus_tag="VLR27_08140"
     CDS             922..>2495
                     /locus_tag="VLR27_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSP03455.1"
                     /translation="MHDLVIRNGTIVDGTGAPRRRADVAIDGERIVAVGDDVGEGRRT
                     IDADGMVVTPGWVDIHTHYDGQVTWDPDLAPSSVNGVTSVVTGNCGVGFAPARPDRHD
                     WLISLLEGVEDIPGTALAEGLSWGWETFPEYLDVLDRHRWTVDVGTQVPHAALRTYVM
                     GARGADHTERATAEDIDAMSRLCEAGIRAGALGFTTSRTWVHRTSTGESIGTLTAATD
                     EVLGIADALTRAGTGVIQLISDAYQSDDDELVARELELLGELALKVGRPLSFTVQQND
                     DTPDRFRQLLTAIGSWVEAGADAKAQVAVRPIGVLLGLDASANPLLFAPTWSRLPTDR
                     AARLAELRTPEVRERLREEHAAAAGHLRGFPLIIHSGWDRMYPLADPPDYEPTPEHSV
                     AGLAAAQGREPFDVLYDLLTGPAATGEGSTLLYVPLMNYANGSLDDVHEMLSSPHAMV
                     GLSDAGAHCNAISDGTMPTTAITHWTRDRTRGPKLSLEHMVHHQTQRTAAHVGWHDRG
                     VVAPGYLADLNVIDLDR"
BASE COUNT          361 a         1001 c          817 g          316 t
ORIGIN      
        1 ttcgacttcc cgctcatgca ggccatgtac ctggcgctgg cccgctgcga cgccggcccg
       61 atccgcggtg cgctggaggc ccgaccggag atccccgagg acgcccagtg ggccatcttc
      121 gtgcgcaacc acgacgagct caccctcgac cagctcagcg acgacgagcg ccaggaggtg
      181 ttcgacgcct tcggccccga ggagcacatg cagctgtacg gccgtgggct gcgccggcgc
      241 ctgccaccga tgctggacgg cgaccaggac cgcatccgga tggtgtacag cctgctgttc
      301 agcctccccg gcacgccggt gctgttctac ggcgaggaga tcggcatggg cgagaacctc
      361 gacatcgagg gtcgcctcag cgtgcgcacg ccgatgcagt ggaccgccgg caagaacgcc
      421 gggttctcct cggcccggcc gtcccgcctg gcccgcccgg tgcccgacgg ccagttcggt
      481 ccgctggcga tcaacgtcgc cgaccagcgc cggcagtccg actcgctgct cacgtggatg
      541 gagcggatga tccgccggcg ccgcgagacg cccgagctcg gctggggcgc gacgatgctg
      601 ctcgacaccg gtgacgatgc cgtgctcgcc caccgctgcg actgggagcg gtccgccgtg
      661 gtcgcgatcc acaacctcag cccgaccccc cgggagctgc gcctcgacct gggcccgatg
      721 gagggctgcg accaggtcgt cgacctgctc gacgccgcgc agccggtcca cgaggtcgac
      781 ggctccacca tcgacctcac cctggccggc tacgcccacc actggttccg gctccaggac
      841 agctcgggct ccacgccgcc gtaggaccgc ggctcccggt cgaggctacg gtcgttgcca
      901 ggacgacctt cgggggacga catgcacgac ctggtgatcc gcaacggcac gatcgtcgac
      961 ggcacgggcg caccgaggcg ccgcgccgac gtcgccatcg acggcgagcg catcgtggcg
     1021 gtgggcgacg acgtcggcga gggccggcgc accatcgacg ccgacggcat ggtcgtcacg
     1081 cccggctggg tcgacatcca cacccactac gacggtcagg tcacctggga tcccgacctc
     1141 gcgccgtcgt cggtgaacgg cgtgacctcg gtcgtcaccg gcaactgcgg ggtcggcttc
     1201 gcccccgccc ggcccgaccg ccacgactgg ctcatctcgc tgctcgaggg cgtggaggac
     1261 atccccggca ccgcgctggc cgagggcctc tcgtggggct gggagacctt ccccgagtac
     1321 ctcgacgtgc tcgaccgcca ccgctggacc gtcgacgtcg gcacccaggt cccccacgcc
     1381 gcgctccgca cctacgtcat gggcgcccgg ggcgccgacc acaccgagcg ggccaccgcc
     1441 gaggacatcg acgccatgag ccggctgtgc gaagccggca tccgcgccgg ggcgctcggg
     1501 ttcaccacca gccgcacgtg ggtgcaccgc accagcaccg gcgagtcgat cggcaccctc
     1561 accgccgcca ccgacgaggt cctcggcatc gccgacgccc tcacccgggc cggcaccggc
     1621 gtcatccagc tcatctccga cgcctaccag tccgacgacg acgagctggt cgcccgcgag
     1681 ctcgagctcc tcggcgagct ggcgctcaag gtgggccggc cgctgtcgtt caccgtccag
     1741 cagaacgacg acacccccga ccgcttccgg cagctgctca ccgccatcgg gagctgggtc
     1801 gaggcaggcg ccgacgccaa ggcccaggtg gccgtacggc cgatcggcgt gctgctgggg
     1861 ctcgacgcgt cggccaaccc gctgctgttc gccccgacct ggtcccggct gccgaccgac
     1921 cgcgccgccc gcctcgccga gctgcgcacc cccgaggtcc gcgagcgcct gcgggaggag
     1981 cacgcggccg ctgccgggca cctgcggggg ttcccgctga tcatccactc gggctgggat
     2041 cgcatgtacc cgctggccga cccgcccgac tacgagccga cgcccgagca cagcgtcgcc
     2101 ggcctcgccg ccgcccaggg ccgcgagccc ttcgacgtcc tctacgacct gctcaccggt
     2161 ccggccgcca ccggcgaagg ctcgacgctg ctctacgtgc cgctcatgaa ctacgcgaac
     2221 ggcagcctcg acgacgtcca cgagatgctc tcgtcgccgc acgccatggt cggcctgtcc
     2281 gacgccggcg cccactgcaa cgccatctcc gacggcacca tgcccaccac ggccatcacg
     2341 cactggaccc gcgaccgcac ccggggcccg aagctgagcc tcgagcacat ggtccaccac
     2401 cagacccagc gcaccgccgc ccacgtcggc tggcacgacc gcggtgtggt ggcacccggc
     2461 tacctggccg acctcaacgt catcgacctc gaccg
//
LOCUS       DATISU010000197         3388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_149461, whole genome shotgun sequence.
ACCESSION   DATISU010000197 DATISU010000000
VERSION     DATISU010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149461"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VLR27_08145"
     CDS             complement(<1..1034)
                     /locus_tag="VLR27_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HSP03456.1"
                     /translation="MITATSIVVVLVAVPLVTAFIGYITNWAAVRMIFQPEEFVGFWK
                     IGWQGVLPSKADTFAADVAANVGAVLSPAELAERLDPAEMEALFADRLDDELPVIVRD
                     VAEMLAPGTWDQMAPEAQQMVITQIKTEGRQVAQEIFDDLQGISHELLDLEQLVFDLL
                     SGPNVKRLVRLFQEIGGKELKAIVRFGGYFGFVIGCFQALSFSVFDQWWLMPLVGGIV
                     GLGTNWLALQMIFRPIEPKRYAGLVTYQGMFPSRQEEIAQDYGRITAREILTPANLLR
                     LVTEGEAGTRIAQVVLTKVSERVDAARPMVEMLTQTEVTDAQLASVKMMVVGRVTESV
                     PEIQPEIEAYL"
     gene            complement(1073..1912)
                     /gene="purU"
                     /locus_tag="VLR27_08150"
     CDS             complement(1073..1912)
                     /gene="purU"
                     /locus_tag="VLR27_08150"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HSP03457.1"
                     /translation="MTQTVLLLRCPDRPGIVAAVGQTIAALGGNIIHADQHTDRVHGL
                     FLQRVEATFPDGADIEGAMRPVAAAYDMAWEIHRPGPPPRIAVLCSKQGHCLADLLSR
                     VDLGELDAHIEFVASNHDDHEGLVARHGVDFHLLPVAPDDPSRQSADMHALVRDAEVD
                     LVVLARYMRILPEAFIADFPNRIINIHHSFLPAFVGAKPYHQAYERGVKLIGATAHYA
                     TGELDQGPIIHQDVTPVSHRHEVTDLIRLGTDLEKSVLARAVRLHLEHRVLAYGNRTV
                     VFD"
     gene            1950..2948
                     /locus_tag="VLR27_08155"
     CDS             1950..2948
                     /locus_tag="VLR27_08155"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSP03458.1"
                     /translation="MRRALWTTDGLDVTEVEPGPLAEGWARLRVAACGICGSDLHWWH
                     GSMPRPLGTAPGHELAGTVVDGPAGLDDVLYAVSPNVSCGRCQFCQVGQTHLCDRALG
                     RGLGMGADGGLSEVLDVPVANLAPVTTGSPVLASLTEPLAVALRGVSLGDIGPDSRVL
                     VLGAGTIGLGAALLARDRASEVAITARHPHQADAAAAMGVTVLGEHEGITWAKERGAD
                     VVIESVGGTAPTLGEAVKAARKGGTIVLLGSFTEPRPVDLSKLMMKELRLQGSFCYGS
                     TKRGSEYAAAAQLTGRFESELSAMTTHQFGLDQVTDAFEAAADKSSGAIKVTILPN"
     gene            complement(3005..3078)
                     /locus_tag="VLR27_08160"
     tRNA            complement(3005..3078)
                     /locus_tag="VLR27_08160"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3042..3044),aa:Ala,seq:cgc)
     gene            complement(3127..>3388)
                     /locus_tag="VLR27_08165"
     CDS             complement(3127..>3388)
                     /locus_tag="VLR27_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03459.1"
                     /translation="EAASELRRNVLDRLRAGDLEGGEALLEVMDDAYDVLASVDLPDA
                     LTGGLRRTVDSLRAVTERTRGDVTTTVLQTRLQRAIESHGDA"
BASE COUNT          487 a         1159 c         1242 g          500 t
ORIGIN      
        1 aggtaggcct cgatctcggg ctggatctcc ggcactgatt cggtgacccg acccacgacc
       61 atcatcttca ccgaggcgag ctgggcgtcg gtgacctcgg tctgggtcag catctcgacc
      121 atcggccggg cggcatcgac ccgctcgctc accttggtga gcacgacctg ggcgatgcgg
      181 gtgccggcct cgccctcggt gaccagtcgg agcaggttgg ccggcgtgag gatctcgcgg
      241 gcggtgatcc ggccgtagtc ctgcgcgatc tcctcctggc ggctcgggaa catgccctgg
      301 taggtgacga ggccggcgta gcgcttgggc tcgatcgggc ggaagatcat ctgcagggcg
      361 agccagttgg tgccgaggcc gacgatgccg ccgaccagcg gcatcagcca ccactggtcg
      421 aagaccgaga agctcagcgc ctggaagcag ccgatcacga agccgaagta cccgccgaag
      481 cggacgatgg ccttgagctc cttgccaccg atctcctgga acaggcggac gaggcgcttc
      541 acgttcgggc cggagagcag gtcgaacacg agctgctcca ggtcgagcag ctcgtggctg
      601 atgccctgca ggtcgtcgaa gatctcctgc gcgacctggc ggccctcggt cttgatctgg
      661 gtgatgacca tctgctgcgc ctcgggcgcc atctggtccc aggtgcccgg cgccagcatc
      721 tcggccacgt cgcggacgat gacgggcagc tcgtcgtcca gccggtcggc gaacagcgcc
      781 tccatctcgg ccgggtcgag gcgctcagcc agctcggcgg gcgacagcac ggcgccgacg
      841 ttggcggcga cgtcggcggc gaaggtgtcg gccttggagg gcagcacgcc ctgccagccg
      901 atcttccaga agcccacgaa ctcctcgggc tggaagatca tgcggaccgc ggcccagttg
      961 gtgatgtagc cgatgaacgc ggtgacgagg ggcaccgcga cgaggacgac gacgatgctg
     1021 gtcgcggtga tcacaggcca agtttcgccg acggggcggg gttcacctgt ccctagtcga
     1081 agacgaccgt gcggttgccg taggcgagca cgcggtgctc gagatggagc cgcacggccc
     1141 gggccagcac ggacttctcg aggtcggtgc cgagccggat gaggtcggtg acctcgtggc
     1201 ggtggctgac cggggtgacg tcctgatgga tgatcgggcc ctggtccagc tcgccggtgg
     1261 cgtagtgggc ggtggcgccg atgagcttca cgccccgctc gtaggcctgg tggtacggct
     1321 tggcgcccac gaaggccggc aggaacgagt ggtggatgtt gatgatccgg ttcgggaagt
     1381 cggcgatgaa cgcctcgggc aggatccgca tgtagcgggc gagcacgacg aggtcgacct
     1441 ccgcgtcgcg cacgagggcg tgcatgtcgg ccgactggcg gctcgggtcg tcgggggcga
     1501 cgggcagcag gtggaagtcc acaccgtggc gggccacgag tccttcgtgg tcgtcgtggt
     1561 tggaggcgac gaactcgatg tgggcgtcga gctcccccag gtcgacccgg gacagcaggt
     1621 cggcgaggca gtggccctgc ttggagcaca gcacggcgat gcggggcggt ggcccgggcc
     1681 ggtggatctc ccaggccatg tcgtaggcgg cggcgaccgg ccgcatggcg ccctcgatgt
     1741 cggcgccgtc ggggaaggtg gcctcgaccc gctgcaggaa cagcccgtgc acacggtcgg
     1801 tgtgctggtc ggcgtggatg atgttgccgc cgagcgctgc gatcgtctgg ccgacggccg
     1861 cgacgatccc cggccggtcg gggcagcgga gcagcagcac ggtctgggtc accccggaaa
     1921 ccctagggag cgggggctac ggtccggaca tgcgccgtgc cctgtggacc accgacggac
     1981 tcgacgtgac cgaggtcgag cccggacccc tcgccgaggg ctgggcgcgc ctgcgcgtcg
     2041 cggcctgcgg catctgcggc tcggacctcc actggtggca cggatcgatg ccccgcccgc
     2101 tcggcaccgc acccgggcac gagctggccg gcaccgtcgt ggacggtccg gccggcctcg
     2161 acgacgtgct ctacgccgtg tcgcccaacg tgtcctgcgg gcgctgccag ttctgccagg
     2221 tcggccagac ccacctgtgc gaccgagcac tcggccgggg cctgggcatg ggcgccgacg
     2281 gcggcctgtc cgaggtcctc gacgtgccgg tcgccaacct cgcgccggtc accaccggat
     2341 cgcccgtcct cgcctcgctc accgagccgc tggccgtcgc gctgcggggc gtgtcgctcg
     2401 gcgacatcgg ccccgacagc cgggtgctgg tgctcggcgc cggcaccatc ggcctcggcg
     2461 ccgcgctgct ggcccgcgac cgcgcctccg aggtcgccat caccgcccgc cacccgcacc
     2521 aggccgacgc cgcagccgcc atgggcgtca cggtcctcgg cgagcacgag ggcatcacct
     2581 gggccaagga gcggggcgcc gacgtcgtca tcgaatccgt cggcggcact gcgcccaccc
     2641 tcggcgaggc cgtcaaggcc gcccgcaagg gcggcaccat cgtcctgctc ggctccttca
     2701 ccgagccccg cccggtcgac ctgtcgaagc tgatgatgaa ggagctccgc ctgcagggct
     2761 cgttctgcta cggctccacc aagcggggct cggagtacgc cgccgctgcc cagctcaccg
     2821 gtcgcttcga gtcggagctg tcggcgatga ccacgcacca gttcggcctc gaccaggtca
     2881 ccgacgcgtt cgaggccgcg gccgacaagt ccagcggcgc catcaaggtc accatcctcc
     2941 ccaactgaaa atgtcgtgga tcggtcacca ctggtgtgac ggatccacga cattttcctc
     3001 cgggtggagg tgaggggatt cgaacccctg gcctcctcga tgcgaccgag gcgctctagc
     3061 caactgagct acacccccga gggaggccac accctacagc gaggccggtt cgcgtccgaa
     3121 cccggctcag gcgtcgccgt gggactcgat ggcgcgctgc aggcgggtct ggagcacggt
     3181 ggtggtgacg tcgccccggg tgcgctcggt gacggcccgc agggagtcca cggtgcggcg
     3241 cagaccgccg gtgagggcat cgggcaggtc gacggaggcg aggacgtcgt aggcgtcgtc
     3301 catcacctcg aggagggctt cgccgccttc gaggtcgccg gcgcgcagcc ggtccaggac
     3361 gttgcggcgc agctcgctgg ccgcctcg
//
LOCUS       DATISU010000198        12499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_289087, whole genome shotgun sequence.
ACCESSION   DATISU010000198 DATISU010000000
VERSION     DATISU010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 12499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12499
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289087"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VLR27_08170"
     CDS             complement(<1..436)
                     /locus_tag="VLR27_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HSP03460.1"
                     /translation="MTTALGIDVGTTNVKAAIVGADGTVHATASRNLVADGEGARAEL
                     DADVQWDAVVDAVRECVVAAPEAAAEIVAVGVDSQYSSIVPVDAGGRPVAPMRLWSDR
                     RGTERSFAILAAHDDAFMGFIERHGIPPVGGGLSLGHVLHLQH"
     gene            complement(501..1874)
                     /locus_tag="VLR27_08175"
     CDS             complement(501..1874)
                     /locus_tag="VLR27_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HSP03461.1"
                     /translation="MPTSTNPATGEQIATYDEHTADEVTGFVEEAHARHLEWRRTSFD
                     ERAGVLRATADALDARADELAELMAAEMGKPLAAGRSEVEKCAWACRYYADHGAEHLA
                     DVPIEADRRRSYIHHDPLGVVLAVMPWNFPLWQVFRFAAPALMAGNAGLLKHASNVTG
                     CALAIEDLFRTGGAPEGLFRTLVISSRHVEAVLEHPLVRAATLTGSVPAGSAVAEAAG
                     RLLKKTVLELGGSDPYVVLADADVEAAAKTCATSRLINSGQSCIAAKRFIVVDEVHDA
                     FVDAFVAEMAAAVMGDPMDDETTVGPQAQESLRDDLHDQVRRTIDAGAELVLGGEVPD
                     RDGAWYPPTVLTGVTSDMAAGSEETFGPVGAVMRARDEDHAIELANDTSFGLGAAVFT
                     QDLERGERIAAEELAAGCCFVNDLVASDPRLPFGGIKDSGYGRELSHLGIREFVNEKT
                     VAVGPKQ"
     gene            2008..2268
                     /locus_tag="VLR27_08180"
     CDS             2008..2268
                     /locus_tag="VLR27_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03462.1"
                     /translation="MSDGTFATDPAAAADLVGDLQAAARQIDGQCLADPGGDAVFGAA
                     TGALAAIPSAGARWRGDCADVLIALAAGVALARSGTVAADSW"
     gene            2265..4109
                     /locus_tag="VLR27_08185"
     CDS             2265..4109
                     /locus_tag="VLR27_08185"
                     /inference="COORDINATES: protein motif:HMM:NF018018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP03463.1"
                     /translation="MTAADLARASERLQVLRGVAAATAADTAALASTVAGRADATGWW
                     VGPQGDDLRAGLRQITDDARTTTGAWESLAGTVQGLQATATALAGELAALERQRDAAQ
                     DTLSRLWRTARSVVEDAFDVDAGVAEEQAALARTEHAIAEVQRRWVLACQAAAPALDG
                     ALAAVATGVSSRATIRLGGLMLPTGAALDLLVGLLEGAPPPGADPGAVATWWADLSVA
                     ERAVWLETAPQVIGNLDGVPFPVRAEANRITMQALVDTLPAGDPLLERLSQFLDDQGR
                     IDPTRSIIVFDPAGDGTVAELFGDLDAAAHIAVIVPGMGSTMANFTNGVSRDARALYT
                     ATPGSAVISWTGYDAPAGAETGRVWEVATSAQAIAGGAALVPFLAAVRGDRRADLSVI
                     GHSYGSHTVGQGLLQGGQADRVVFIGSPGVGVQHVSEFPAGAAREYFAGEVKGDPVAT
                     LERFGDAPTDPDFGAFAYDAGSPDSGNPIGRHSEYFDEGIAIDNLSTIVSGGIPSPDQ
                     PRLVERWLEAHEDVHDGVHGVVDGLQDLVHVPVAEPAVDGVIDGVQHVDRTTRQVTAV
                     VGEVAGHYATEAGHWVWDRGGDLAGAAGDVIDSSFDRMRTLDRLTSWR"
     gene            4119..4589
                     /locus_tag="VLR27_08190"
     CDS             4119..4589
                     /locus_tag="VLR27_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03464.1"
                     /translation="MLRSALAAVALALSLTACSESEGTVAPDAAAAPAGVAEAVAAEV
                     HEALGVSDDIEVDDPIVEPCYDSTGRDTGEVSARVHSRAEPGVVTQERFEGLGERLGA
                     EDVRRNDLRDPLDETQVIGQAQQLFADRDGRAVFVSMQLTDEDGFSASIRAECP"
     gene            complement(4647..6044)
                     /locus_tag="VLR27_08195"
     CDS             complement(4647..6044)
                     /locus_tag="VLR27_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HSP03465.1"
                     /translation="MSSLYPYADRFPVNRTLPDEGRSREEILDELRSLAASEDAAWET
                     GKCSGSMYCGDHDHYGFMNEVFGLFGHMNILQRDMCPSATRFEGEVIAMALDLMHGDA
                     AAERGHDIAGMVTSGGSGSIMHALLAYRDHARQERGITNPNIVKPETSHPAFDKGCHL
                     FGIEARNVPVDPDTTTVRPEVVAGQIDENTVAIVGSACNYGYGTIDPIPALSDLAVER
                     GVGLHVDGCLGGFILPFGQELGYDQIPTFDFRLPGVTTISADTHKYGYGLKGTSTLLF
                     RDKEVRNSQYFFRTDWTGGKYCSPGMDGSRSGGLLAATWASMVSLGRSGYLAYAKQIF
                     ETSFAMQEAVRSHPELRILGDATFLFAFTSDEFDIYHVNDFMRLRGWRFNGQQYPNAI
                     HMAVTRPQTQPGVVEAFAADLDEAVAYALEHKDESPKSAAVYGGVAGGMTDEADEFIR
                     SVMGDMMDKHQGLPG"
     gene            complement(6080..7183)
                     /locus_tag="VLR27_08200"
     CDS             complement(6080..7183)
                     /locus_tag="VLR27_08200"
                     /inference="COORDINATES: protein motif:HMM:NF037526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HSP03466.1"
                     /translation="MSPTTSADPLLTELLERRGAPVATIVAAGPGFEGDVQSRLDIRS
                     KEARKQLEEAGVSAAAVDAVEAAIQSHTGEDGLGIVADRNGVLFTTLLEVDTDSEILQ
                     VEALPRYVPFVRERFVNRPHVVVRCDRVGAQIARVERGEIQRDTEVVGDDEHVSKVHA
                     GGWSQRRFQNRAEHTWDQNAKEIAEAVVTEADAIDAELVIVTGDGRAVQLVAEHLPER
                     FADHLVLDDHQPFDDESDAVVFDRAMTLVHDRVGRELVEVLERFGELRGQNEGAADDV
                     SDVLAALRQGAVETLLVSGDSDERVHMALEDPMQIAFDAAGITDLGLEDVADGRLTDA
                     AIQSALTGGAAVMIVPEHGPNSPNGPLGAILRF"
     gene            complement(7258..8055)
                     /locus_tag="VLR27_08205"
     CDS             complement(7258..8055)
                     /locus_tag="VLR27_08205"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HSP03467.1"
                     /translation="METITVRRPTFDLEDLPRRWVGGSRLGTWFGNAGHVFIPLGEQF
                     FIDSVRPFRDDLDDAELRRAVSAFIGQEAVHARVHTAVWDQLQDQGVPVEQYAGLITG
                     FRRLTEAHVPPALRLSTTAALEHYTAAFGRAFLDEDLDGVLPTGMGELLRWHGAEELE
                     HRSVAYDVLTEVDDDLALRVAGLVLGSALLLVVPGVGVALFAASDLARGRVRPGARLP
                     NRRLLGMGGRLLARFARDVASYVHPSFHPGDDPPPASYDTWRAAQPS"
     gene            8166..9185
                     /locus_tag="VLR27_08210"
     CDS             8166..9185
                     /locus_tag="VLR27_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03468.1"
                     /translation="MVTSAWPLAWSIAAILIGGAVLLTAGVRLTRVADTLADRTGLGE
                     AMAGAVLLGAVTSLPGLVTTVVGAASGEAGFAVSNAVGGIAAQTTFLALADLVYRRVN
                     LEHAAASIPNLTQALILVSLIGLVLGGTGSPDITLFAVHPVTIILVAVYAYGLVLTNR
                     AHEHPMWQPRQTEDTVEDVAEPPSDRSNRQVWTEFVGLAAVVAVVGWVIGTAGLSIAD
                     RTGLSGSLVGATFTAVTTSLPELVTVITAVRIGAVTLAVGDIIGGNTFDVLFIAAADI
                     AFRDGSIYHAADPQALFLMSVTVVLTATLAAGMVGREERHIGFEGVAILVFYVLGLAS
                     LVAIG"
     gene            complement(9196..9951)
                     /locus_tag="VLR27_08215"
     CDS             complement(9196..9951)
                     /locus_tag="VLR27_08215"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP03469.1"
                     /translation="MTGRVTIDEGVVYGQAGGDGRDLRCDVYRPEDHDGPLPGVLLIH
                     GGGWRQGDRTQLRGYGVLIGREGYVCVAPEYRLVPESPWPAQIDDVRAALQWMRDHAD
                     QLGIDPTRIAVEGNSAGAHLALLLAADESLQLGACIAVYPPTHLVFGEHVDGSVPLIA
                     IADDGGSLELEREVSPLLLASPRFPPTKLIHGSGDDVVPVAASFRMYEALHAHGVPVD
                     LHIYAEQPHAFDAEPLYGRATAREMVHFLGRYL"
     gene            complement(9977..11176)
                     /locus_tag="VLR27_08220"
     CDS             complement(9977..11176)
                     /locus_tag="VLR27_08220"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSP03470.1"
                     /translation="MHDGIRVLDLTQYLAGPACTRLLAEMGAEVIKVEAAPYGDPSRA
                     FTPRRNRRAGFFVQQHRGKQSICLDLSTDEARSIVRELADEVDVVVENFSPGVMDRKG
                     WGYEELSSTNPGLVMCSVSGFGQTGPLRDKPAFDFVAQAYGGVMHMTGDPDGPPTFVG
                     IGLADTVAGVHAFAAIGWALLRRTMTGRGTHIDASMVDSLVHLHETSIYAPSITDGEY
                     VPMRAGRHYQPSSPGGTFQGPEGWIVVFCTQGQIQGLWDALGHPEYGEDERFRNNDGR
                     LANRDELTEILEAWMAGFDTDDDVIAALEAHRVPCSKVLSPAEFVDQPHLVERGMIRS
                     VDDPIAGPVTIPGFPFAFDGERPQLDTPAPLLGEHNRSVLTGLLGWDDERVDALEAAG
                     VLQSKPR"
     gene            complement(11198..11971)
                     /locus_tag="VLR27_08225"
     CDS             complement(11198..11971)
                     /locus_tag="VLR27_08225"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HSP03471.1"
                     /translation="MKLQWKVAIVTGAASGIGAHAARLFAGEGATVVCVDRDHHGANA
                     VVNEITAAGGRGLALTVDVSAEDEMRGMVERTVEEFGRLDVLFNNAGVMLADDGDAET
                     TTEDVWDQTLAINVKGVWLGCKYGIPAMRDAGGGSIINTASFVALMGAATPQLAYTAS
                     KGAVLSMTRELAVLHAREGIRVNALCPGPLRTKLLMDFLDTDEKKQRRLVHVPMGRFG
                     EAEEMAKAALFLATDDSSYMTGATFTVDGGITSAYVTPE"
     gene            complement(12104..>12499)
                     /locus_tag="VLR27_08230"
     CDS             complement(12104..>12499)
                     /locus_tag="VLR27_08230"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HSP03472.1"
                     /translation="SQVVNVALGGSLHVHLPDVVDGTVVHRKEPEALRGMPGPTPHEI
                     EVETGSLIAEVMGADRVTPMSWHHQAVDRVGDGLRIVARASDGTIEALEHDTHPWLAI
                     VQWHPELTAAGDPTQQRLFDALVARAADG"
BASE COUNT         1662 a         4462 c         4606 g         1769 t
ORIGIN      
        1 cgtgctggag gtgcagcacg tgaccgaggc tgaggccacc cccgaccggc gggatgccgt
       61 ggcgctcgat gaagcccatg aacgcgtcgt cgtgcgcggc gaggatcgcg aagctgcgtt
      121 cggtgcctcg gcggtccgac cagagccgca tcggcgcgac cggtcggccg ccggcgtcga
      181 ccgggacgat cgaggagtac tggctgtcga caccgacggc cacgatctcg gccgcggcct
      241 ccggcgcggc gacgacgcac tcgcggaccg cgtcgacgac cgcgtcccac tggacgtcgg
      301 cgtcgagctc ggcccgcgcg ccctcgccgt cggcgacgag gttgcggctg gcagtggcgt
      361 gcaccgtgcc gtcggcgccg acgatggccg ccttgacgtt ggtggtgccg acgtcgatcc
      421 ccagcgcagt ggtcacggcc gacatccaac ccgaaaatct cgtctgctgg tcacatctgt
      481 ggtgaccagc agacgaaaga tcactgcttg gggccgaccg ccacggtctt ctcgttcacg
      541 aactcgcgga tgccgaggtg gctgagctcc cggccgtagc cggagtcctt gatgccgccg
      601 aagggcaggc gggggtcgga ggcgaccagg tcgttcacga agcagcagcc ggcggccagc
      661 tcctcggcgg cgatgcgctc accccgctcg aggtcctggg tgaacacggc ggcaccgagc
      721 ccgaaggacg tgtcgttggc gagctcgatg gcgtggtcct cgtcgcgggc tcgcatcacg
      781 gcgcccacgg ggccgaaggt ctcctccgac ccggccgcca tgtcggaggt gacgccggtg
      841 agcacggtgg gcgggtacca ggcgccgtcc cggtcgggga cctcgccgcc gaggaccagc
      901 tcggcgccgg cgtcgatcgt gcgccggacc tggtcgtgca ggtcgtctcg cagggactcc
      961 tgggcctggg gcccgaccgt ggtctcgtcg tccatcgggt cccccatcac ggccgccgcc
     1021 atctcggcga cgaaggcgtc gacgaaggcg tcgtggacct cgtcgacgac gatgaagcgc
     1081 ttggccgcga tgcaggactg gcccgagttg atgagccggc tggtggcgca ggtcttcgcc
     1141 gccgcctcca cgtcggcgtc ggccagcacg acgtaggggt cgctgccgcc cagctccagc
     1201 accgtcttct tcagcagccg tcccgcggcc tcggcgacgg ccgacccggc gggcacgctg
     1261 ccggtgagcg tcgcggcgcg gaccagcggg tgctccagga ccgcctcgac gtggcgcgag
     1321 gagatgacga gggtgcggaa cagcccctcg ggcgcgccgc ccgtgcggaa caggtcctcg
     1381 atggcgagcg cgcacccggt cacgttcgag gcgtgcttga gcagtccggc gttgccggcc
     1441 atcagggcgg gagcggcgaa ccggaacacc tgccacaggg ggaagttcca cggcatgacg
     1501 gcgaggacga cgccgagggg atcgtggtgg atgtagctgc gccggcggtc ggcctcgatc
     1561 gggacgtcgg ccaggtgctc ggcgccgtgg tcggcgtagt accggcacgc ccacgcgcac
     1621 ttctccacct cgctgcgccc ggcggccagc ggcttgccca tctcggccgc catgagctcg
     1681 gccagctcgt cggcccgagc gtcgagggca tcggcggtgg ctcgcagcac cccggcgcgc
     1741 tcgtcgaacg aggtccggcg ccactcgagg tgccgggcgt gggcctcctc gacgaagccg
     1801 gtgacctcgt cggcggtgtg ctcgtcgtag gtggcgatct gctcgccggt ggccgggttc
     1861 gtgctggtcg gcatccctgt gccgtaccca ctccggcccg gcgacgttcg ggcaggaggc
     1921 agcggcggtg ccgtcgtatc ctcagacggg acccgtgagc gggaccacgg cggaacgtgc
     1981 aggggatgac atggcgcgag gcgacacatg agcgacggga ccttcgccac cgaccccgcg
     2041 gccgcggccg acctcgtcgg cgacctgcag gccgccgccc gccagatcga cgggcagtgc
     2101 ctcgccgacc ccggcgggga cgccgtgttc ggtgcggcga cgggcgcgct ggcggcgatc
     2161 ccgtcggcag gggcccgctg gcgaggtgac tgcgccgacg tcctcatcgc gctcgccgcc
     2221 ggcgtcgccc tcgcccggtc cgggacggtg gctgccgact cgtggtgacc gccgccgatc
     2281 tcgcccgggc atcggagcgg ctccaggtcc tgcggggcgt ggccgccgcg acggccgccg
     2341 acaccgcggc cctggccagc accgtcgccg gccgggccga cgccaccggg tggtgggtgg
     2401 gaccccaggg cgacgacctc cgggccggcc tccgtcagat caccgacgat gcccgcacca
     2461 ccacgggtgc gtgggagtcc ctcgccggca cggtgcaggg gttgcaggcg accgcgaccg
     2521 cgctggccgg cgagctcgcc gccctcgagc gccagcggga cgccgcacag gacacgctct
     2581 cccgcctgtg gcggacggcg cgctcggtgg tcgaggacgc gttcgacgtc gacgccggtg
     2641 tcgccgagga gcaggcggcg ctggcccgca ccgagcacgc catcgccgag gtccagcggc
     2701 gctgggtcct ggcctgccag gccgctgctc ccgcgctcga cggtgcgctc gccgcggtgg
     2761 ccaccggcgt cagcagcaga gcgacgatcc gcctcggtgg cctgatgctg cccacgggcg
     2821 cggcgctcga cctcctggtc ggcctgctcg agggggcccc gccgcccggc gccgacccgg
     2881 gtgccgtcgc gacctggtgg gccgacctca gcgtcgccga gcgtgccgtc tggctggaga
     2941 ccgcccccca ggtgatcggg aacctcgacg gcgtcccctt cccggtgcgc gccgaggcga
     3001 accggatcac gatgcaggcg ctggtcgaca ccctccccgc cggcgacccc ctgctcgagc
     3061 ggctgtcgca gttcctcgac gaccagggcc ggatcgaccc cacgcgctcg atcatcgtct
     3121 tcgatccggc aggcgacggg acggtggccg agctcttcgg tgacctcgac gccgccgcgc
     3181 acatcgccgt catcgtgccc ggcatgggct ccaccatggc caacttcacc aacggcgtga
     3241 gccgcgacgc ccgtgccctc tacacggcga ctccgggctc agccgtcatc tcctggaccg
     3301 ggtacgacgc gccggcgggt gccgagaccg gccgggtctg ggaggtcgcg acgagcgcgc
     3361 aggcgatcgc cggcggggcc gcgctcgtgc cgttcctcgc tgcggtgcga ggggatcgcc
     3421 gcgccgacct cagcgtcatc ggccactcct acggcagcca caccgtcggc caggggctgc
     3481 tgcagggcgg ccaggccgac cgggtggtgt tcatcggcag ccccggcgtc ggcgtccagc
     3541 acgtctccga gttccccgcc ggcgcagccc gggagtactt cgccggcgag gtgaagggcg
     3601 acccggtcgc gaccctggag cgcttcggcg acgccccgac cgatcccgac ttcggcgcct
     3661 tcgcctacga cgccggctcg cccgactccg gcaacccgat cggtcgccac tccgagtact
     3721 tcgacgaggg catcgcgatc gacaacctgt cgaccatcgt ctccggcggg atcccgtccc
     3781 cggaccagcc ccggctcgtc gagcgctggc tggaggccca cgaggacgtc cacgacggcg
     3841 tgcacggggt cgtcgacggc ctgcaggacc tggtgcacgt ccccgtggcc gaaccggcgg
     3901 tggacggcgt gatcgacggc gtgcagcacg tggaccggac cacccgccag gtcaccgcgg
     3961 tcgtcggtga ggtggccggc cactacgcca ccgaggccgg tcactgggtg tgggatcgtg
     4021 gaggcgacct cgccggggcc gccggcgacg tcatcgactc cagcttcgat cgcatgcgca
     4081 ccctcgaccg cctcacctca tggagatgac cccctcccat gctccgttcg gcgctggccg
     4141 ctgtcgcact cgccctgtcc ctcaccgcct gctcggagtc ggagggcacc gtggcgcctg
     4201 acgccgccgc cgcgccggcc ggggtcgccg aggccgtcgc cgccgaggtc cacgaggcgc
     4261 tcggcgtcag cgacgacatc gaggtcgacg accccatcgt cgagccctgc tacgactcga
     4321 cgggccgcga caccggcgag gtgagcgcgc gggtgcacag ccgggccgag cccggtgtcg
     4381 tcacccagga gcgcttcgag gggctcggcg agcgcctcgg ggccgaggac gtccgacgca
     4441 acgacctgcg cgaccccctc gacgagaccc aggtcatcgg gcaggcgcag cagctgttcg
     4501 ccgaccgcga cggtcgtgcc gtcttcgtgt cgatgcagct caccgacgaa gacggcttct
     4561 ccgcctcgat ccgcgccgag tgcccctgac cgacccgtcc cgcacccgcg gccaggagct
     4621 tctggcagcg accacggaac gaccggtcac ccgggcaggc cctggtgctt gtccatcatg
     4681 tcgcccatca cgctgcggat gaactcgtcg gcctcgtcgg tcatgccgcc ggcgaccccg
     4741 ccgtagacag cggccgactt cggcgactcg tccttgtgct cgagcgcgta cgccaccgct
     4801 tcgtccaggt ccgcggcgaa ggcctcgacc acgccgggct gggtctgggg gcgggtgacg
     4861 gccatgtgga tggcgttggg gtactgctgg ccgttgaagc gccacccacg cagtcgcatg
     4921 aagtcgttca cgtggtagat gtcgaactcg tcgctggtga aagcgaaaag gaacgtggcg
     4981 tcgccgagga tccgcagctc ggggtggctg cgcacggcct cctgcatggc gaagctggtc
     5041 tcgaagatct gcttggcgta ggccaggtag ccgctgcggc ccaggctcac catcgacgcc
     5101 caggtggcgg cgagcagccc gccggagcgg gagccgtcca tgcccgggga gcagtacttg
     5161 ccgcccgtcc agtcggtgcg gaagaagtac tggctgttgc gcacctcctt gtcccggaac
     5221 agcagcgtgg acgtgccctt cagcccgtag ccgtacttgt gggtgtcggc cgagatggtg
     5281 gtgacgccgg gcaggcggaa gtcgaaggtc gggatctggt cgtagcccag ctcctggccg
     5341 aaggggagga tgaagccgcc gaggcacccg tcgacgtgca ggcccacgcc gcgctccacc
     5401 gcgaggtccg acagcgcggg gatcgggtcg atggtgccgt agccgtagtt gcaggccgaa
     5461 ccgacgatgg cgacggtgtt ctcgtcgatc tggccggcca cgacctcggg ccgcacggtc
     5521 gtggtgtcgg ggtccacggg cacgttgcgg gcctcgatgc cgaacaggtg gcagcccttg
     5581 tcgaaggccg ggtggctggt ctcgggcttg acgatgttgg ggttcgtgat gccgcgctcc
     5641 tggcgggcgt gatcgcggta cgccagcagg gcgtgcatga tcgaacccga gccccccgag
     5701 gtgaccatgc cggcgatgtc gtggccgcgc tcggcggccg cgtcgccgtg catcaggtcg
     5761 agggccatgg cgatgacctc gccctcgaag cgggtggcgc tcgggcacat gtcccgctgg
     5821 aggatgttca tgtggccgaa caggccgaac acctcgttca tgaacccgta gtggtcgtgg
     5881 tcgccgcagt acatcgaccc cgagcacttg ccggtctccc acgcggcgtc ctcggacgcg
     5941 gcgagggacc gcagctcgtc gaggatctcc tcgcggctgc ggccctcatc gggcagcgtg
     6001 cggttgacgg ggaaccggtc ggcgtagggg tacagcgagc tcatggccgc ggaccgtacc
     6061 ggccggggcg tgccctcgct cagaagcgca ggatggctcc gagcggaccg ttcgggctgt
     6121 tgggaccgtg ctcgggcacg atcatgacgg cggccccacc ggtgagcgcc gactggatgg
     6181 cggcatcggt gagccggccg tcggcgacgt cctcgagacc gaggtcggtg atgccggcgg
     6241 cgtcgaaggc gatctgcatc ggatcctcga gggccatgtg gacccgctcg tcggagtcac
     6301 ccgacaccag cagcgtctcg accgcgccct ggcggagcgc ggcgagcacg tcggagacgt
     6361 cgtcggcggc gccctcgttc tggccacgca gctcgccgaa gcgctcgagc acctccacca
     6421 gctcccggcc gacccggtcg tgcacgaggg tcatggcccg gtcgaagacc acggcgtcgg
     6481 actcgtcgtc gaagggctgg tggtcgtcga ggaccaggtg atcggcgaag cgctcgggga
     6541 ggtgctcggc caccagctgc acggcgcggc cgtcgccggt gacgatgacg agctcggcgt
     6601 cgatggcgtc ggcctcggtg accaccgcct cggcgatctc cttcgcgttc tggtcccacg
     6661 tgtgctcggc gcggttctgg aagcgccgct ggctccagcc gccggcgtgc accttgctga
     6721 cgtgctcgtc gtcgccgacg acctcggtgt cgcgctggat ctcgccgcgc tcgacgcggg
     6781 cgatctgggc gccgacccgg tcgcagcgca ccacgacgtg gggccggttg acgaagcgct
     6841 cgcgcacgaa gggcacgtag cggggcaggg cctcgacctg gaggatctcg ctgtcggtgt
     6901 cgacctcgag cagcgtggtg aacaggacgc cgttgcggtc ggcgacgatg ccgagcccgt
     6961 cctcaccggt gtgggactgg atggcggcct cgacggcatc gacggcggcg gccgagacgc
     7021 cggcctcctc gagctgcttg cgggcttcct tggatcggat gtcgagccgg gactgcacgt
     7081 ctccctcgaa gccgggaccg gcggcgacga tggtggcgac gggcgctccc cggcgctcga
     7141 gcagctcggt gagcagcggg tcggcagagg tggtcggcga cacatggact ccttgatcgt
     7201 ggggtgggaa cgcccggtcc gtacccgacg ccggatcccc ccaccacgcc gtccggccta
     7261 ggagggttgc gccgctcgcc aggtgtcgta ggacgccggc ggcgggtcgt cgcccgggtg
     7321 gaacgacggg tggacgtagc tggcgacgtc ccgggcgaag cgggccagga gccggccgcc
     7381 catgccgagc aagcggcggt tgggcagccg tgcgccgggg cggacgcgac cccgggccag
     7441 gtcggaggcg gcgaagaggg cgacgccgac gccggggacg acgaggagga gggcgctgcc
     7501 gagcaccagg ccggccacgc ggagggcgag gtcgtcgtcc acctcggtga gcacgtcgta
     7561 ggcgacgctg cggtgctcca gctcctcggc accatgccag cgcagcagct cgcccatgcc
     7621 ggtcggcagc acgccgtcga ggtcctcgtc gaggaacgcc cggccgaagg ccgccgtgta
     7681 gtgctcgagc gcagcggtgg tggagaggcg caacgcgggc ggcacgtggg cctcggtgag
     7741 gcgacggaag ccggtgatga ggccggcgta ctgctcgacc gggacgccct ggtcctgcag
     7801 ctggtcccag accgcggtgt gcacccgggc gtggacggcc tcctggccga tgaaggcgct
     7861 gaccgcccgg cgcagctcgg catcgtcgag gtcgtcccgg aacggccgga ccgagtcgat
     7921 gaagaactgc tcccccagcg ggatgaacac gtggccggcg ttgccgaacc aggtcccgag
     7981 gcggctcccg ccgacccaac gccgggggag gtcctcgagg tcgaaggtgg gccgccggac
     8041 cgtgatcgtc tccacgtccc catcctgtcc gctttcaccc ggttcggacc gcacccagct
     8101 ggcgatggcg tcgatcaacg gcccgaagag actgccgcgc cggcccctcg ggtagtggcg
     8161 ttggcatggt cacgagcgcc tggccgctgg cgtggagcat cgcggcgatc ctcatcggcg
     8221 gcgccgtgct gctcaccgcc ggcgtgcgcc tcacgcgcgt cgccgacacc ctcgccgatc
     8281 gaaccggcct cggcgaggca atggcgggcg cggtgctcct cggcgcggtg acgtcgctgc
     8341 ccggcctggt cacgaccgtg gtcggcgccg ccagcgggga ggcgggcttc gccgtcagca
     8401 acgcggtcgg cggcatcgcg gcccagacca cgttcctcgc gctcgccgac ctcgtgtacc
     8461 gccgggtcaa cctcgagcac gcagcggctt ccatccccaa cctcacccag gcgctcatcc
     8521 tggtcagcct catcggtctg gtgctgggcg ggaccggcag cccggacatc acgctgttcg
     8581 ccgtgcaccc ggtcaccatc atcctcgtgg ccgtctacgc ctacggcctg gtcctcacca
     8641 accgggccca cgagcacccg atgtggcagc cccgccagac cgaggacacc gtcgaggacg
     8701 tcgccgagcc cccgagcgac cgctcgaacc gccaggtctg gaccgagttc gtcggcctcg
     8761 ccgccgtcgt ggccgtcgtc ggctgggtca tcggcaccgc cggcctctcg atcgccgacc
     8821 gcaccgggct gtccgggtcg ctcgtcgggg ccacgttcac cgcagtgacg acctcgctgc
     8881 ccgagctggt caccgtcatc accgccgtgc gcatcggtgc cgtcaccctc gccgtcggcg
     8941 acatcatcgg cggcaacacc ttcgacgtgc tgttcatcgc cgcggccgac atcgccttcc
     9001 gggacgggtc gatctaccac gccgccgatc cgcaggccct cttcctcatg tcggtgaccg
     9061 tggtgctcac cgccacactc gccgccggca tggtcgggcg cgaggagcgc cacatcggct
     9121 tcgagggcgt ggcgatcctg gtcttctacg tgctcgggct ggcgtcgctc gtcgccatcg
     9181 gctgagcgcg tcggatcaga ggtagcggcc caggaagtgc accatctccc gggcggtggc
     9241 ccgcccgtac aggggctcgg cgtcgaaggc gtggggctgc tcggcgtaga tgtgcaggtc
     9301 gaccggcacg ccgtgggcgt ggagcgcctc gtacatgcgg aagctggccg ccaccggcac
     9361 gacgtcgtca ccgctgccgt ggatgagctt ggtcggcggg aaccggggtg aggcgaggag
     9421 cagggggctg acctcgcgct cgagctcgag ggatccgccg tcgtcggcga tggcgatcag
     9481 cgggaccgac ccgtcgacgt gctccccgaa caccaggtgc gtcggcgggt acacggcgat
     9541 gcacgcgccg agctgcagcg actcgtccgc ggccagcagc agcgccaggt gcgcgccggc
     9601 ggagttgccc tcgaccgcga tgcgggtcgg gtcgatgccg agctggtcgg cgtggtcgcg
     9661 catccactgg agggcggcgc gcacgtcgtc gatctgggcc ggccacggcg actccgggac
     9721 gagccggtac tccggcgcga cgcacacgta gccctcccgc ccgatgagca cgccgtagcc
     9781 acggagctgc gtgcgatcgc cctgccgcca accaccaccg tggatcagca gcaccccggg
     9841 gagcggaccg tcgtggtcct cgggccggta cacgtcgcag cgcagatccc gcccgtcacc
     9901 cccggcctgc ccgtagacca cgccctcgtc gatcgtcacc cgtccggtca tggcccggca
     9961 gtctcgcgcc cggcggtcag cggggcttgg actggaggac gcccgccgcc tcgagggcgt
    10021 cgacgcgctc gtcgtcccag ccgagcaggc ccgtgaggac cgagcggttg tgctcgccga
    10081 gcagaggagc cggcgtgtcg agctgcgggc gctcgccgtc gaaggcgaac gggaagccgg
    10141 ggatggtgac cggcccggcg atcgggtcgt ccaccgaccg gatcatgccc cgctcgacca
    10201 ggtggggctg gtcgacgaac tccgccggcg acagcacctt cgagcacggc acccggtggg
    10261 cctcgagcgc ggcgatgacg tcgtcgtcgg tgtcgaagcc ggccatccac gcctcgagga
    10321 tctcggtgag ctcgtcccgg ttggccaggc ggccgtcgtt gttgcggaac cgctcgtcct
    10381 cgccgtactc ggggtgcccg agcgcgtccc agagcccctg gatctgaccc tgcgtgcaga
    10441 acaccacgat ccagccctcg ggtccctgga acgtgccgcc cggagacgac ggctggtagt
    10501 gccggccggc ccgcatcggc acgtactcgc cgtcggtgat cgagggggcg tagatcgagg
    10561 tctcgtgcag gtggacgagg gagtccacca tcgacgcgtc gatgtgggtg ccccggccgg
    10621 tcatcgtgcg gcggagcagg gcccagccga tggcggcgaa cgcgtggacg ccggcgacgg
    10681 tgtcggccag gccgatgccg acgaacgtgg gcgggccatc ggggtcaccc gtcatgtgca
    10741 tcacgccgcc gtaggcctgg gccacgaagt cgaacgccgg cttgtcccgc agcgggccgg
    10801 tctgcccgaa gccggacacc gagcacatga ccaggccggg gttggtcgag gacagctcct
    10861 cgtagcccca gcccttgcgg tccatgacgc cggggctgaa gttctcgacc acgacgtcga
    10921 cctcgtcggc cagctcgcgc acgatcgagc gggcctcgtc ggtggacaga tcgaggcaga
    10981 tgctctgctt gccccggtgc tgctggacga agaagccggc gcgccggttg cgccgggggg
    11041 tgaaggcgcg gctcgggtcg ccgtaggggg cggcctcgac cttgatgacc tcggcaccca
    11101 tctcggcgag cagccgggtg caggccgggc cggccaggta ctgggtgagg tcgaggaccc
    11161 ggatgccgtc gtgcacgttc cgctgccgct gccagagcta ctcgggggtg acgtaggcgg
    11221 aggtgatgcc cccgtccacg gtgaaggtcg ccccggtcat gtacgaggag tcgtcggtgg
    11281 cgaggaacag cgcggccttg gccatctcct cggcctcgcc gaagcggccc atcggcacgt
    11341 gcaccagccg gcgctgcttc ttctcgtcgg tgtcgaggaa gtccatgagc agcttcgtgc
    11401 gcagcggccc ggggcacagc gcgttgaccc ggatgccctc gcgggcgtgg agcacggcca
    11461 gctcgcgggt catggagagc accgcgccct tcgaggcggt gtaggccagc tgcggggtgg
    11521 ccgcgcccat gagggcgacg aaggaggcgg tgttgatgat cgagccgccg ccggcgtcgc
    11581 gcatggccgg gatgccgtac ttgcagccga gccagacgcc cttgacgttg atggccagcg
    11641 tctggtccca cacgtcctcg gtggtggtct cggcgtcgcc gtcgtcggcg agcatgaccc
    11701 cggcgttgtt gaagagcacg tcgaggcggc cgaactcctc caccgtccgc tcgaccatgc
    11761 cccgcatctc gtcctcggcc gagacgtcga cggtgagggc caggccccga ccgcccgccg
    11821 cggtgatctc gttgaccacg gcgttggccc cgtggtggtc gcggtcgacg cagacgaccg
    11881 tcgcgccctc gccggcgaac agccgggcgg cgtgggcgcc gatgccgctc gccgcacccg
    11941 tgacgatggc gaccttccac tggagcttca tgtggtcagg atgccacccg ggggctgaac
    12001 atcggcgcgg acgaatcacg tcccgctgct gtccgatctg acggctccgc cgtcacaggg
    12061 cccgtgacat gggtcgtctc gcctgccggc tcggctcctg ggctcagccg tcggcggccc
    12121 gggcgacgag cgcgtcgaac agccgctgct gggtcggatc gcccgccgcg gtgagctccg
    12181 gatgccactg cacgatcgcg agccagggat gggtgtcgtg ctccagggcc tcgatcgtgc
    12241 cgtcggaggc gcgcgccacg atgcgcaggc cgtcgcccac ccggtcgacc gcctggtggt
    12301 gccagctcat gggcgtgacc cggtcggcac ccatcacctc ggcgatcagc gagccggtct
    12361 ccacctcgat ctcgtgcggc gtcggaccgg gcatgccgcg cagggcctcg ggctccttgc
    12421 ggtggacgac ggtgccgtcg accacgtcgg gcaggtggac atggaggctg ccgccgaggg
    12481 cgacgttcac gacctggga
//
LOCUS       DATISU010000199        13911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_475602, whole genome shotgun sequence.
ACCESSION   DATISU010000199 DATISU010000000
VERSION     DATISU010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 13911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13911
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475602"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VLR27_08235"
     CDS             complement(<1..747)
                     /locus_tag="VLR27_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSP03473.1"
                     /translation="MLLDAIDHVQLAMPPGGEELATWFYEGILGVPRVPKPPHLAVRG
                     GCWFERGALRIHLGVQDDFVPATKAHPAFTVLDLDRLEATITTHGVAVRRVDDPLEPD
                     VLYVDDPFGNRIELRPTRGTEDKADYAEVVEGVARSAVGDRLADELAFVHELDRLKTV
                     LRQSFVGDASRRENTAEHSWHVAVMAAVLSEHLEADVDLARVVLLLLVHDIVEIDAGD
                     TGIYDDTAAGSKEGREVAAADRLYGLLPDDV"
     gene            773..2191
                     /locus_tag="VLR27_08240"
     CDS             773..2191
                     /locus_tag="VLR27_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HSP03474.1"
                     /translation="MALDAARIRTSVDASWDRDVLGPLQDYVRIPNVSPAFDPHWEAN
                     GHMADATALIRQWCEARPIEGMTVEVLEVPGRTPIVLCEIPATDPALAEDTVLLYGHL
                     DKQPEMSGWRDDLGPWEPVLEGDLLYGRGGADDGYAVFSSLTAIEALREAGGSHARLL
                     VLIEASEESGSPDLPAHLDALGDRLGQVSLVIGLDSGAATYDRLWVTTSLRGVLMASV
                     RVEVLTEGVHSGAAGGVVPSSFRLLRRLLDRIEDPDTGRVLVEELWADIPAEREAQME
                     ATADQITAGLAFPWAGTTRPDHADAVDLLRARTWEPSIANIAVDGFPPADRAGNVLRP
                     FTELTLSCRLAPTADPVAAADALERALTADPPNGATVTVAVRGAEGGWNAPPTADWLK
                     AAVDEASQAHWGQPSAAMGEGGTIPFMGMLGARFPEAQFLITGVLGPGSNAHGPNEFL
                     HLPMARRITATVSHVLDAHTRR"
     gene            2193..4712
                     /gene="hrpB"
                     /locus_tag="VLR27_08245"
     CDS             2193..4712
                     /gene="hrpB"
                     /locus_tag="VLR27_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HSP03475.1"
                     /translation="MADHGRLPVGDLPIAEAIEPLRAALADRGRAVLHAPPGAGKTTI
                     VPLVLLEEPWCGGTVLVLEPRRLAVRNATRRLAQLLGEQPGERVGWRMRQDTKVSRRT
                     RIEVITEGVLTRRLQRDPELAGVSAVVFDEFHERSLDADLGLALTLEVADALRPELRI
                     VVMSATLDIEPVAALLDAPVVRSEGRAFPVDVVHVDPDPGSRDRRRAAEDAVAPTVRR
                     ALAAHDGDVLVFLPGGAEIRRAERALGQLAPDVDVRPLHGSMPPAAQDAAIAPSPPGR
                     RKVVLSTAIAETSLTIEGVTVVVDAGLRRTPRLDPGSGMSRLVTLPVTAAEAEQRAGR
                     AGRLAPGTAYRLWSRAEDATRRSHPEPEIEVADLTSLALELAAWGADEGDLAWLTPPP
                     PAHLAAARDLLHRLGALDEGRTITPHGRAMAEQPLHPRLAHLLLRGAELGHGGLAADL
                     AAALHEHGLSRRRSTDVAERVRALRSPGDHDRGAVERARRDARRWRAAVGAEGRPGDT
                     VGTAEDDVLGLVLALGFPDRIARARAVRPDGSGRGELLLANGRGAFLEPTDPLAASEW
                     VVVAELDGQATRSRVYEAAALDEADVRTVAGDSIEITTEVGWDDRSGDVVARSVERLG
                     AITLAERPVDDAPPEALAAALVAGIRREGTSLLPWDDDTRRLQARLSFLHRLEPSRWP
                     DVADEVLVAEADERIGPMLVGMRRRRDLRRLDLREVLQAGLDWRDRGAIDDLAPERLP
                     VPSGNRHRVDYSVDPPVLAVKLQEVFGATETPAVGGGKVPVVLHLLSPAGRPLQVTQD
                     LPSFWRGAYAEVRADMRGRYPKHPWPEDPMAAPPTARTKRR"
     gene            4762..5139
                     /locus_tag="VLR27_08250"
     CDS             4762..5139
                     /locus_tag="VLR27_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSP03476.1"
                     /translation="MDLDDAARKQLESLGGFIRERRKQAQYSLRDLADRANVSNPYLS
                     QIERGLHTPSVRVLKAIAAALNVSAESLLVQAGLLEASDDGEPGPSVEDAIRADGLLS
                     DDQKAAMLAVYRSYVARNTAEDD"
     gene            complement(5197..5514)
                     /locus_tag="VLR27_08255"
     CDS             complement(5197..5514)
                     /locus_tag="VLR27_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03477.1"
                     /translation="MSTIMTTVTTYEDKALELIVKVQDEVLGYVKQAVQILDDRLPEV
                     ELPEIAVLDQVPTLEEVVTTQFAFSKKLLTNQEKFAKNVVKAVKPLTRDAKPKAKTAT
                     KAA"
     gene            complement(5616..9125)
                     /locus_tag="VLR27_08260"
     CDS             complement(5616..9125)
                     /locus_tag="VLR27_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSP03478.1"
                     /translation="MQCPQCRAEVPVGARFCSSCGHELVMVGDQRRIATVLFADLVGF
                     TALSEELDPEQVKALVDRCFERLAEDIEDFGGRVDKVVGDAIVALFGAPVAHEDDAER
                     AVRAGLRMQQTIAGMAAEGGVPVRMRVGINTGEVLVGTVRAGREYTAMGDVVNTASRL
                     QEAAEPGTVVVGPETRMATRDSFEYADIDPVLAKGKRLPVHASIAVRAVAPPGQRLRH
                     NRAPLIGRETELALLAGVTETAIDRSRAALLLVHGEAGMGKSRLAVEVAEHARNDLGA
                     FVLEGRCVPYGEANVWWPVAEGLRVSVGLSSDATPDEVDAALRKAATDALTDSGRLDE
                     LERVVAGLHHLLGHDGALRGIDATRAREEAIRSVVALAEGFSQRKPLFVVLSDLHWAD
                     QEVLDLIDTLLVRLARRPFVLLATARRALRERWSPSPAAANSVVLHLDPLSEEASQRL
                     LAHLSDGTLHPSMAAMLVERSGGNPFYLEELVALVTEAGVRTIDGLLTSEGGELPSTL
                     RGLVAARLDALAPGDRKILDDAAVLGRRNRTDALITMAQVKRGVDAETARAHLESLAA
                     REFLVFDGDLYTFRSEVIREVAYSTLTKADRARSHAGIARWIVDHEDHDREHIVDQIA
                     NHWAAAAELVAEIGSVTDVPDDVCAEAVDWLHRASLRAEADVHPATVEALTTRALGLM
                     DDEHPDRAEVLRLRARARVDRYQLDAALQDAEVAEVLATATGDERALGDILLVRGDAL
                     HHRGAFAEALEALARAKAHFAAAEHVAGCAEAERLQGMVHLFGGDFVAADDALRTAHG
                     LFEEVDDRRGMAWALQNLSWSAYIAGRIDEAEHRVTAAMATFEELGDPGGLTWSRGLL
                     AWVRFHQGRNDEALALATAVGDDAHQRGERWGEAMMRVLSASISLWSGRTEDAVESAH
                     QAVALFEEIDDQIGELQARCGLGRALLMAGHVESGLVELRSALADGVGPDGDEQGWMA
                     RVSMLMAAVQLGDVDLGEAALADSKGFADTDSVGFGDRVGALALHHLQQGDVAAASAV
                     LEQHVEPRGYVQAARALVAAGGGDAETAQCLADELVTLPHTYLDRAYAEIARGIALAV
                     SDRAGEARTVLAQAIADLDSTGDRLAQAIVRLVDASVAEQAHRTDAATARGVAEARLR
                     DLGVEANGWRHLLEDALGLLATR"
     gene            9242..11824
                     /locus_tag="VLR27_08265"
     CDS             9242..11824
                     /locus_tag="VLR27_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSP03479.1"
                     /translation="MWRVTLRSLWEHKRRLVSTVLAIVLGVAFMSGTFVFADTIDKVF
                     ADLFGETRSGVDVQVQGVELFDGGFGGQDARELLDIGLVDAVAAVEGVTVAEPFIQTV
                     GFGSQNRVLGPDGEPLGAAAGPPTLIESWVESDQLNPYRIATGEPPSRDDEVALNVAA
                     AEDAGFDLGDEVRVVSQLGEATYRLVGTFTFGEAESAAGAVSADFTLAEVQRLAGLEG
                     RTQVVLAAGDGSVDEEALTQRVASVLPPTAEALTGTEAAEQNASDVQEGFGFFRQLLS
                     IFAGIALLVGTFIIANTFAILVAQRTRELALLRAIGASRRQVLTSVLLEAAVIGLVAA
                     VLGLLGGIALATGVTAALEASGADLPTSGLVISTTTVVTALVLGLGITLLASVVPAVQ
                     ATRVPPLAALRDVAIDRSGASKPRIVVGVVLLAIGAWSLSSAWRVAEPEVATVGLGAL
                     VLIVGAIVIGPVLAEPSVRVLGAGLPRLRGITGVLATENAARSPKRTSATASALLIGV
                     ALIGFITVFAASARESVVAEVERGFTGDLIIQSESSGFGPPGGFPPAVADTVASIEGV
                     EATSRIGFTQAQVTYPDGRAGTQFVQSVDPTTLSQAFDARMAAGEVTDLGPGGVLVDV
                     GVAEDNGIRVGDVVLLTGTGGQSLELEVRALSDDLTLLGFWSIDTADYERLVNERQLV
                     QVIATVSDGADVAEVQAAVEAALVGVPAIDVLDRDGFIGSLTDQITSFLTLVNALLAL
                     SVIIAMIGIANTLSLSIHERTRELGLLRAVGMTRSQLRSAVRWEAVLISVLGTLVGLS
                     LGMVVSWALVRSLESFGLTRFTVPVGQLVVVVLVAAALGVIASIRPARRAARLDVLDA
                     IAVE"
     gene            11845..12240
                     /locus_tag="VLR27_08270"
     CDS             11845..12240
                     /locus_tag="VLR27_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HSP03480.1"
                     /translation="MARTPAELTDADLEFLAERHLATLTTLRPDGSPHVVAVGFTWDH
                     DAGIARVITWDASKKHRNVAEQPGGRAVVCQVDGGRWLSLEGPATAVTDPDACRDAEA
                     RYAQRYREPKSREDRSVIEIRVDRILGRS"
     gene            complement(12283..13773)
                     /locus_tag="VLR27_08275"
     CDS             complement(12283..13773)
                     /locus_tag="VLR27_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03481.1"
                     /translation="MATAALAAVAWGTGAVAPAQADPAGLVQLPGAIASANMEYVGTI
                     PIESPGVSHAVREVGDQRLLYVNGAKGLTIYDIDDPADPQRVGFLLLPHSQNEDIQVS
                     EDGTRAIIAADGGLLVPIQETRGLHIVDTSDPKAPEVVAWLDNANGGTNHTATCADDA
                     CEWIYGNRGAIYHVPADTDGDEDIRTLENSWFDLDERVTSSHALNRSVHADPETLQPV
                     TIMISDSEPRLVMDATDPANPVLLATSDPADHEGDGFLQHNNTRPNAADWTPRSLDGD
                     TARGPAGGLPTTASPKANGVPGGTQHAAVLPDGTDPFGGHLRPGELVIGNSESNIVPQ
                     CDDQSGGLTTFSIADFDQGATMEPLHTFRPVNGDWLDGNPGANGLGCSGHWFDIRDGD
                     TLIAASWYEHGVKVIEVLPDYSMQEIGFFQPVVGAAGAASWVVDDDGTEYIFSTDYER
                     GLDIIRFHRDAPRATEAEMRTAALAVTQTNPVADAERLICELGGQG"
BASE COUNT         1745 a         5148 c         5074 g         1944 t
ORIGIN      
        1 gacgtcgtcg ggcagcaggc cgtagagccg atcggctgcg gcgacctcgc ggccctcctt
       61 ggacccggcc gcggtgtcgt cgtagatgcc ggtgtcgccg gcgtcgatct ccacgatgtc
      121 gtggacgagc agcaggagga ccacccgggc caggtcgacg tcggcctcga ggtgctcgct
      181 cagcacggcg gccatcaccg cgacgtgcca gctgtgctcg gcggtgttct cgcggcggga
      241 cgcgtcgccc acgaaggact ggcgcagcac ggtcttgagc cggtccagct cgtggacgaa
      301 ggcgagctcg tcggccagcc gatcgcccac cgcgctccgg gccacgccct cgacgacctc
      361 ggcgtagtcg gccttgtcct cggtgccacg ggtcggtcgc agctcgatgc ggttgccgaa
      421 cgggtcgtcc acgtagagga cgtcgggttc cagcgggtcg tccactcggc gcacggccac
      481 gccgtgcgtg gtgatcgtgg cctccaagcg gtcgaggtcg agcacggtga aggccgggtg
      541 ggccttggtc gccgggacga agtcgtcctg caccccgagg tggatgcgca gagcgccccg
      601 ctcgaaccag catccgcccc ggacggcgag gtgcggtggc ttcgggacgc ggggcacacc
      661 gaggatgcct tcgtagaacc aggtggccag ctcctcgccg ccgggcggca tggcgagctg
      721 gacgtggtcg atcgcgtcga ggagcacccg tccatcctgc tagcaaagag ccgtggcact
      781 cgacgcagcc cggatccgca cctcggtgga cgccagctgg gaccgcgacg tcctcggtcc
      841 gctccaggac tacgtgcgca tccccaacgt ctcccccgcc ttcgacccgc actgggaggc
      901 caacggccac atggccgacg ccaccgccct gatccgccag tggtgcgagg cccgtcccat
      961 cgaggggatg acggtcgagg tcctcgaggt gcccgggcgc acgccgatcg tgctgtgcga
     1021 gatcccggcc acggacccgg cgctggccga ggacaccgtc ctgctctacg gccacctcga
     1081 caagcagccg gagatgtcgg ggtggcgtga cgacctcggc ccgtgggagc cggtgctcga
     1141 gggcgacctg ctgtacggcc ggggcggtgc tgacgacggc tacgccgtgt tctcgtcgct
     1201 caccgccatc gaggccctgc gcgaggcggg tggcagccac gcccgcctgc tggtgctcat
     1261 cgaggccagc gaggagtcgg gcagccccga cctgcccgcc cacctcgatg ccctcggcga
     1321 ccggctcgga caggtctccc tcgtgatcgg gctcgactcg ggtgctgcca cctacgaccg
     1381 gctctgggtc acgacgtcgc tccggggcgt gctcatggcc tcggtccggg tcgaggtcct
     1441 caccgagggc gtccactccg gcgccgctgg cggcgtggtg ccgtcctcgt tccggctgct
     1501 gcgccggctg ctcgaccgca tcgaggaccc cgacaccggc cgggtgctgg tcgaggagct
     1561 gtgggccgac atccccgccg agcgcgaggc ccagatggag gccactgccg accagatcac
     1621 ggccgggctc gccttcccgt gggccgggac gacccggccc gaccacgccg atgccgtcga
     1681 cctgctccgg gcccgcacgt gggagccctc gatcgccaac atcgcggtcg acggcttccc
     1741 tcctgcggac cgggccggca acgtgctgcg ccccttcacc gagctcaccc tctcgtgccg
     1801 cctcgccccc accgccgacc cggtggcggc agccgacgcc ctcgagcggg ccctcaccgc
     1861 cgacccgccc aacggcgcca ccgtcacggt cgccgtgcgc ggcgccgagg gcgggtggaa
     1921 cgcgccgccc accgccgact ggctgaaggc cgccgtggac gaggcatcgc aagcgcactg
     1981 gggccagccg tcggcggcga tgggcgaggg cggcaccatc ccgttcatgg gcatgctcgg
     2041 cgcccggttc cccgaggccc agttcctgat caccggcgtc ctcggcccgg gctcgaacgc
     2101 gcacgggccg aacgagttcc tgcacctgcc gatggcccgg cgcatcacgg ccacggtcag
     2161 ccacgtgctc gacgcgcaca cccggcgctg acgtggccga ccacggtcgc ctcccggtcg
     2221 gcgacctgcc gatcgccgag gcgatcgagc cgctgcgcgc cgcgctcgcc gaccggggcc
     2281 gggccgtgct gcacgcgcct ccgggcgcgg gcaagaccac catcgtcccg ctcgtgctgc
     2341 tggaggagcc gtggtgcggc gggaccgtgc tggtgctcga accccgccgg ctcgccgtgc
     2401 gcaacgccac gcgccgcctg gcccagctgc tcggcgagca accaggcgag cgggtcggtt
     2461 ggcgcatgcg ccaggacacc aaggtgtcgc gccggacccg gatcgaggtc atcaccgagg
     2521 gcgtgctcac gcgacggctc cagcgcgacc cggagctcgc cggcgtgtcc gcggtggtgt
     2581 tcgacgagtt ccacgagcgc agcctcgacg ccgacctcgg cctggcgctg accctcgagg
     2641 tcgccgacgc gctgcgcccc gagctgcgca tcgtggtcat gtccgccacc ctcgacatcg
     2701 agccggtggc cgcgctcctc gatgcccccg tggtccgctc ggagggccgg gcgttccccg
     2761 tcgacgtcgt ccacgtcgac ccggaccccg ggagccggga tcgtcgccgg gcggccgagg
     2821 atgccgtggc cccgaccgtg cgacgtgccc tcgccgccca cgacggggac gtgctggtct
     2881 tcctgcccgg cggcgccgag atccgccggg ccgagcgggc cctcggccag ctggcaccgg
     2941 acgtcgacgt gcgaccgctg cacgggtcca tgccacccgc cgcccaggac gcggccatcg
     3001 ccccctcccc gcccggccgg cgcaaggtcg tgctgtcgac cgccatcgcc gagaccagcc
     3061 tcaccatcga gggcgtgacg gtcgtcgtcg acgccggcct gcgacgcacg ccccggctcg
     3121 accccggcag cggcatgagc cgcctcgtca ccctcccggt caccgcggcg gaagccgagc
     3181 agcgcgccgg tcgcgccggg cggctcgcac ccggtaccgc ctaccggctg tggagccggg
     3241 ccgaggacgc cacgcggcga tcccaccccg aaccggagat cgaggtggcc gacctgacct
     3301 cgctggcgct cgagctcgcc gcttggggag ccgacgaggg cgacctcgcc tggctgaccc
     3361 cgccgccacc agcgcacctc gccgcggcac gcgacctgct ccatcgcctc ggcgccctcg
     3421 acgagggacg caccatcacg ccgcacggcc gggcgatggc cgagcagccc ctccaccccc
     3481 gcctggccca cctcctgctg cgcggcgccg agctcggtca cgggggcttg gccgccgacc
     3541 tggcagccgc gctccacgag cacggcctct cgcgccgccg cagcaccgac gtggccgagc
     3601 gggtccgggc actgcgttca cccggcgacc acgaccgggg cgcggtcgag cgcgcccggc
     3661 gcgacgcccg ccgctggcgc gccgcagtcg gcgccgaggg gaggccaggt gacaccgtcg
     3721 ggacggccga ggacgacgtg ctcggcctcg tgctcgccct cggcttcccc gatcgcatcg
     3781 cgcgggcgcg ggccgttcgg cccgacgggt ccgggcgggg cgagctgctc ctggccaacg
     3841 gccgtggcgc cttcctcgag ccgaccgacc cgctcgccgc ctccgagtgg gtggtcgtgg
     3901 ccgagctcga cggccaggcg acccgctccc gggtgtacga ggccgcggcg ctggacgagg
     3961 ccgacgtccg caccgtcgcc ggcgactcga tcgagatcac cacggaggtg ggctgggacg
     4021 atcggtccgg cgacgtcgtg gcccgatcgg tggagcgcct cggtgccatc acgctcgccg
     4081 aacgtcccgt ggacgacgcg ccgcccgagg ccctcgccgc cgcgctcgtc gccggcatcc
     4141 gtcgtgaggg cacctcgttg ctgccgtggg acgacgacac ccggcgcctc caggcccgtc
     4201 tgtcgttcct gcaccggctc gaaccgtccc ggtggcccga cgtcgccgac gaggtgctgg
     4261 tggccgaggc cgacgagcgc atcggcccga tgctggtcgg gatgcggcgc cgccgggacc
     4321 tgcgacgcct cgacctccgc gaggtgctcc aggctgggct cgactggcgg gaccgcggcg
     4381 ccatcgacga cctcgcgccc gaacggcttc cggtgccgag cggcaaccgc caccgggtcg
     4441 actactcggt ggacccgccc gtgctggcgg tgaagctgca ggaggtgttc ggcgccaccg
     4501 agaccccggc ggtgggcggg gggaaggtgc cggtggtgct gcacctgctc tcccccgccg
     4561 ggcgcccgct gcaggtcacc caggacctgc ccagcttctg gcgcggcgcc tacgccgagg
     4621 ttcgggccga catgcgtggc cgctatccca agcacccctg gcccgaggac ccgatggccg
     4681 cgccgccgac ggcgcgcacc aagcggcgct gaccggcagt ggtgctacat cgatcaagca
     4741 cgccggtagc atcggaggcc catggacctc gacgatgccg cccgcaagca gctggagtcg
     4801 ctcggcgggt tcatccgcga gcggcgcaag caggcccagt acagcctgcg tgacctggcc
     4861 gaccgggcca acgtctcgaa cccgtacctg agccagatcg agcggggcct gcacaccccg
     4921 tcggtgcggg tcctcaaggc catcgccgcc gcgctcaacg tgtcggccga gtccctgctg
     4981 gtgcaggccg gcctgctcga ggcgagcgac gacggcgagc ccggcccgag cgtcgaagac
     5041 gccatccggg ccgacggcct gctctcggac gaccagaagg ccgccatgct cgccgtctac
     5101 cggagctacg tggcgcgcaa cacggccgag gacgactgag cccgagcgcg acgaaggcgg
     5161 cgccgcagcg ccgccctcct cacatctgct tcgggctcag gcggccttgg tggcggtctt
     5221 ggccttcggc ttggcgtcgc gggtcagggg cttgacggcc ttgacgacgt tcttggcgaa
     5281 cttctcctgg ttggtgagga gcttcttgga gaaggcgaac tgcgtggtga cgacctcttc
     5341 gagggtcggg acctggtcga gcacggcgat ctcggggagc tcgacctcgg ggaggcggtc
     5401 gtcgaggatc tgaacggcct gcttcacgta gccgaggacc tcgtcctgga ccttgacgat
     5461 cagctcgagg gccttgtcct cgtacgtggt cacggtggtc atgatggtgc tcatcgcggt
     5521 gcgctcctgt ggggctcggg gggcttctct accgtgctaa ctctatcaag cacttgcttg
     5581 atgcaccaga tgacctcggt cacaggtgcc gacgatcagc gggtggcgag gaggccgagc
     5641 gcgtcctcca gcaggtgccg ccagccgttg gcctccacgc ccaggtcgcg caggcgtgcc
     5701 tcggccacgc cccgggcagt ggcggcgtcg gtgcggtggg cctgctcggc cacgctggcg
     5761 tcgaccagcc ggacgatggc ctgcgccagg cggtcgccgg tggagtcgag gtcggcgatg
     5821 gcctgggcca gcacggtgcg ggcctcgccg gcgcggtcgc tcaccgccag ggcgatgccc
     5881 cgggcgatct ccgcgtaggc ccggtcgagg taggtgtggg gcagcgtcac gagctcgtcg
     5941 gcgaggcact gcgccgtctc ggcatcgccc ccgccggccg ccaccagggc tcgggcggcc
     6001 tgcacgtagc cgcgcggttc gacgtgctgc tccagcacgg cgctggccgc cgccacgtcg
     6061 ccctgctgca ggtggtggag cgcgagcgcg ccgacccggt cgccgaagcc gaccgagtcg
     6121 gtgtcggcga agcccttgct gtcggccagt gctgcctcgc cgaggtcgac gtcgccgagc
     6181 tgcacggcgg ccatcagcat ggacacgcgg gccatccagc cctgctcgtc gccgtcggga
     6241 ccgaccccgt cggcgagcgc gctgcggagc tcgacgagcc cggactccac gtgcccggcc
     6301 atcagcaggg cccgaccgag cccgcagcgg gcctgcagct cgccgatctg gtcgtcgatc
     6361 tcctcgaaca gcgccacggc ctggtgggcc gattcgacgg cgtcctcggt gcgacccgac
     6421 cacagcgaga tcgacgccga cagcacgcgc atcatcgcct cgccccagcg ctcgccgcgc
     6481 tggtgggcgt cgtcgcccac cgccgtggcc agggcgagcg cctcgtcgtt gcgtccctgg
     6541 tggaaccgca cccaggcgag cagcccccgg gaccaggtca gcccacccgg gtcaccgagc
     6601 tcctcgaagg tcgccatcgc tgcggtgacc cggtgctcgg cctcgtcgat gcgaccggcg
     6661 atgtaggccg accacgacag gttctgcagc gcccacgcca tgccccgccg gtcgtcgacc
     6721 tcctcgaaca ggccgtgcgc ggtgcgcagg gcgtcgtcgg cggcgacgaa gtcgccaccg
     6781 aacaggtgga ccatgccctg gagtcgctcg gcctcggcgc agcccgccac gtgctcggcg
     6841 gccgcgaagt gggccttggc ccgggccagt gcctcgagcg cctccgcgaa ggcaccccgg
     6901 tggtgcaggg cgtcgccccg gaccaggagg atgtcgccga gcgcgcgctc gtcgccggtg
     6961 gcggtggcga ggacctcggc gacctcggcg tcctggagcg ccgcgtcgag ctggtatcgg
     7021 tcgacccggg ctcgggcgcg gaggcggagc acctcggcgc ggtcggggtg ctcgtcgtcc
     7081 atcaggccga gggcccgggt ggtgagggcc tcgaccgttg ccgggtgcac gtcggcctcg
     7141 gcccgcagcg acgcccggtg cagccagtcg accgcctcgg cgcacacgtc gtcgggcacg
     7201 tcggtcaccg agccgatctc ggccaccagc tcggccgcgg ccgcccagtg gttggcgatc
     7261 tgatcgacga tgtgctcgcg gtcgtggtcc tcgtggtcga cgatccaccg ggcgatgccg
     7321 gcgtgggacc gggcccggtc cgccttcgtg agcgtggagt acgcgacctc gcggatgacc
     7381 tcggagcgga acgtgtagag atcgccgtcg aagacgagga actcccgggc ggcgaggctc
     7441 tcgaggtggg cgcgagcggt ctcggcgtcg acgccgcgct tgacctgggc catggtgatc
     7501 agggcgtcgg tgcggttgcg gcggccgagg acggcggcgt cgtcgaggat cttgcggtca
     7561 cccggggcga gcgcgtcgag gcgggccgcc accagaccgc ggagggtgga cggcagctca
     7621 ccaccctcgg acgtgagcag gccgtcgatg gtgcgcacgc ccgcctcggt gaccagggcc
     7681 acgagctctt cgaggtagaa ggggttgccg ccggagcgct cgaccagcat ggcggccatc
     7741 gaggggtgga gcgtgccgtc gctgaggtgg gcgagcagcc gctggctggc ctcctcgctc
     7801 agcggatcga ggtgcagcac gacggagttc gccgcagccg gcgacgggct ccagcgctcc
     7861 cgcagcgccc gacgggcggt ggcgagcagc acgaacgggc gtcgggccag acggacgagg
     7921 agggtgtcga tgaggtcgag cacctcctgg tcggcccagt gcaggtcgga gagcacgacg
     7981 aagaggggct tgcgctggct gaacccctcg gccagggcga ccaccgagcg gatcgcctcc
     8041 tcccgggcgc gagtggcgtc gatgccgcgc agggcgccgt cgtggccgag caggtggtgg
     8101 agcccggcga cgacgcgctc gagctcgtcg aggcgaccgg agtccgtgag cgcgtcggtc
     8161 gccgccttgc gcagcgctgc gtcgacctcg tcgggggtgg cgtcggagga gaggcccacc
     8221 gacacccgca ggccctcggc gaccggccac cagacattgg cttcgccgta ggggacgcag
     8281 cggccctcga gcacgaacgc gccgaggtcg ttgcgggcgt gctcggcgac ctcgacggcc
     8341 agccgggact tgcccatgcc ggcctcgccg tggaccagca ggagcgcggc gcgggagcgg
     8401 tcgatggcgg tctcggtgac gccggcgagc agcgccagct cggtctcgcg accgatgagc
     8461 ggggcccggt tgtgccgcag ccgctggccg gggggcgcca cggcccgcac ggcgatggag
     8521 gcgtggacgg gcaggcgctt gcccttggcc agcacggggt cgatgtcggc gtactcgaag
     8581 gagtcgcggg tggccatgcg ggtctcgggc ccgacgacca cggtgcccgg ctccgccgcc
     8641 tcctggagcc ggctcgccgt gttcacgacg tcgcccatgg ccgtgtactc ccgcccggcc
     8701 cgcaccgtgc ccaccagcac ctcgccggtg ttgatgccca cccgcatgcg gaccggcacg
     8761 ccgccctcgg cggccatgcc ggcgatggtc tgctgcatgc gcagcccggc gcgcaccgcg
     8821 cgctcggcgt cgtcctcgtg ggccaccggc gccccgaaca gcgccacgat ggcgtcgccg
     8881 acgaccttgt cgacccggcc gccgaagtcc tcgatgtcct ccgcgaggcg ctcgaagcac
     8941 cggtcgacca gggccttcac ctgctcgggg tcgagctcct cggagagggc cgtgaacccg
     9001 acgagatcgg cgaagaggac cgtggcgatg cggcgctggt cgccgaccat caccagctcg
     9061 tgcccgcacg aggagcagaa acgggcaccc accggcacct ctgctcgaca ctgggggcac
     9121 tgcacgctgg cagtctacgg ccggacgaga cccgacccgg cgggacatct gccccgtacc
     9181 gtgtcaggaa tgtccacctc cccgacgtcc gccggcgtcg ccgtgccgcc cggggactga
     9241 catgtggcgg gtcacgctcc gctcgctgtg ggagcacaag cgccggttgg tgtcgacggt
     9301 cctcgccatc gtgctcgggg tggccttcat gtcgggcacg ttcgtgttcg ccgacaccat
     9361 cgacaaggtg ttcgccgacc tcttcggcga gacgcgatca ggggtcgacg tgcaggtcca
     9421 gggcgtggag ctgttcgacg ggggcttcgg cggccaggac gcccgcgagc tgctcgacat
     9481 cgggctcgtc gacgccgtcg ctgccgtcga gggcgtcacc gtggccgagc cgttcatcca
     9541 gaccgtgggc ttcggctcgc agaaccgggt gctgggaccc gacggcgaac ccctcggcgc
     9601 agccgccggg ccgccgaccc tgatcgagag ctgggtggag tcggaccagc tgaacccgta
     9661 ccgcatcgcc accggcgagc cgccgagccg tgacgacgag gtcgcgctga acgtcgccgc
     9721 cgccgaggac gccggcttcg acctgggtga cgaggtgcgg gtcgtgtccc agctgggcga
     9781 ggcgacctac cggctcgtgg ggacgttcac cttcggcgag gcggagtcgg ccgccggcgc
     9841 ggtctcggcc gacttcaccc tggccgaagt ccagcgcctg gccgggctcg agggacggac
     9901 gcaggtcgtg ctggccgccg gcgacgggtc cgtggacgag gaggcgctca cccagcgggt
     9961 ggcgtccgtg ctgcccccca ccgccgaggc gctcaccggc acggaagccg ccgagcagaa
    10021 cgcatccgac gtccaggaag gcttcggctt cttccgccag ctgctctcga tcttcgccgg
    10081 catcgccctg ctcgtcggca cgttcatcat cgccaacacc ttcgccatcc tcgtggccca
    10141 gcggacccgg gagctggcgc tgctccgcgc catcggcgcc agccggcgcc aggtgctgac
    10201 ctcggtgctc ctcgaggccg ccgtcatcgg gctggtcgcc gcggtgctgg ggctgctcgg
    10261 cggcatcgcg ctcgccacgg gggtgacggc cgcgctcgag gcctcgggtg ccgacctgcc
    10321 cacctccggc ctggtgatca gcacgacgac ggtggtgacc gcgctggtcc tcgggctcgg
    10381 catcaccttg ctggcgtcgg tggtcccggc cgtgcaggcc acccgcgtgc cgcccctcgc
    10441 cgccctgcgc gacgtggcga tcgaccggtc cggcgcgtcg aagccccgca tcgtcgtcgg
    10501 cgtcgtcctc ctcgccatcg gcgcgtggtc gctgtcgtcg gcctggcggg tggccgaacc
    10561 ggaggtcgcc accgtcgggc tcggcgccct cgtcctcatc gtcggcgcga tcgtgatcgg
    10621 gccggtcctg gccgagccga gcgtgcgggt cctcggtgcc gggctccccc gcctgcgggg
    10681 catcaccggg gtgctcgcca ccgagaacgc tgcccgcagc cccaagcgca cgtcggccac
    10741 cgcgtcggcg ctgctcatcg gcgtggcgct catcgggttc atcaccgtgt tcgccgcctc
    10801 cgcccgggag tcggtggtgg ccgaggtcga gcgggggttc accggcgacc tgatcatcca
    10861 gtccgagtcg agcgggttcg gtccgcccgg cgggttcccg ccggccgtgg ccgacaccgt
    10921 cgcatccatc gaaggcgtgg aggccacgtc gcgcatcggg ttcacgcagg cgcaggtgac
    10981 gtaccccgac ggacgggccg gcacgcagtt cgtccagtcg gtcgatccga cgacgctctc
    11041 ccaggccttc gacgcccgca tggccgccgg cgaggtcacc gacctcgggc ccggcggcgt
    11101 cctcgtcgac gtcggcgtgg ccgaggacaa cggcatccgc gtgggcgacg tggtgctgct
    11161 caccggcacc ggcggtcagt cgttggagct cgaggtccgg gcactgtccg acgacctcac
    11221 gctgctcggc ttctggtcga tcgacaccgc cgactacgag cggttggtca acgagcgtca
    11281 gctggtgcag gtgatcgcga cggtttcgga cggggccgac gtcgccgagg tgcaggccgc
    11341 cgtcgaggcc gccctcgtcg gcgtgcccgc catcgacgtg ctcgaccgcg acgggttcat
    11401 cggcagcctc accgaccaga tcaccagctt cctcaccctg gtgaacgcgc tcctcgccct
    11461 gtcggtgatc atcgccatga tcggcatcgc caacaccctg tcgctgtcga tccacgagcg
    11521 cacccgcgag ctcggcctgc tgcgcgccgt cggcatgacc cgctcgcagc tgcggtcggc
    11581 cgtgcggtgg gaggccgtcc tcatctcggt gctcggcacg ttggtcggcc tgtcgctcgg
    11641 gatggtggtc agctgggccc tggtgcgctc gctcgagtcg ttcgggctca cgcgcttcac
    11701 ggtgccggtc ggccagctcg tcgtggtcgt cctggtcgcc gccgcgctcg gcgtcatcgc
    11761 ctccatccgc ccggcccggc gggccgcccg cctcgacgtc ctggacgcga tcgcggtgga
    11821 gtaggcggct gccaggctga cgggatggca cgcacacccg ccgagctcac cgacgccgac
    11881 ctcgagttcc tcgccgaacg gcacctcgcc accctgacca cgctgcgacc cgacggctcg
    11941 ccgcacgtcg tggcggtcgg gttcacgtgg gatcacgacg ccggcatcgc ccgggtcatc
    12001 acgtgggatg cgtccaagaa gcaccgcaac gtcgccgagc agcccggtgg ccgggccgtg
    12061 gtctgccagg tcgacggcgg gcgctggctg tcgctggagg ggccggcgac cgccgtcacc
    12121 gaccccgacg cgtgccggga cgccgaagcc cgctacgccc agcgctaccg cgagcccaag
    12181 tcccgggagg accgctcggt catcgagatc cgcgtcgacc ggatcctcgg ccgctcctga
    12241 cggccctccg gaactggcag ttgtgttgtc gcgcgcggcg cctcagccct ggccgccgag
    12301 ctcgcagatc aagcgctcgg cgtcggcgac cgggttcgtc tgggtcaccg ccagcgccgc
    12361 ggtccgcatc tcggcctcgg tggcccgggg ggcgtcgcgg tggaaccgga tgatgtccag
    12421 cccccgctcg tagtcggtcg agaagatgta ctcggtgccg tcgtcgtcca cgacccacga
    12481 cgcggcgccg gccgcaccga cgaccggctg gaagaagccg atctcctgca tcgagtagtc
    12541 gggcagcact tcgatgacct tcacgccgtg ctcgtaccag ctggccgcga tcagggtgtc
    12601 gccgtcgcgg atgtcgaacc agtggccgga gcagccgagg ccgttggctc ccgggttgcc
    12661 gtccagccag tcgccgttga cgggccggaa ggtgtgcagc ggctccatgg tggcgccctg
    12721 atcgaagtcg gcgatcgaga acgtcgtgag gccgccggac tggtcgtcgc actgggggac
    12781 gatgttggac tcggagttgc cgatcaccag ctcgccgggg cggaggtggc cgccgaaggg
    12841 gtcggtgccg tcgggcagga cagcagcgtg ctgcgtgcca cccgggaccc cgttggcctt
    12901 cggcgacgcc gtggtcggga gcccaccggc gggccctcgc gccgtgtcac cgtcgaggct
    12961 ccggggcgtc cagtcggcag cgttggggcg ggtgttgttg tgctggagga agccgtcgcc
    13021 ctcgtggtcg gccgggtcgg acgtcgccag cagcaccggg ttcgccgggt cggtggcgtc
    13081 catgaccagg cggggttcgg agtcgctgat catgatcgtg accggctgga gcgtctccgg
    13141 gtcggcgtgc accgaccggt tgagggcgtg gctgctggtg acccgctcgt cgaggtcgaa
    13201 ccagctgttc tcgagcgtgc ggatgtcctc gtccccgtcg gtgtccgccg gcacgtggta
    13261 gatcgcgccc cggttcccgt agatccactc gcaggcatcg tcggcgcagg tcgcggtgtg
    13321 gttcgtgccg ccgttcgcgt tgtcgagcca tgccacgacc tcgggtgcct tcgggtcgga
    13381 ggtgtcgacg atgtggagcc cacgagtctc ctggatcgga accagcaggc cgccgtcggc
    13441 agcgatgatg gcccgcgtcc cgtcctcgga gacctggatg tcctcgttct gggagtgggg
    13501 caggagcagg aagccgaccc gctgggggtc ggcgggatcg tcgatgtcgt agatcgtgag
    13561 gcccttggcg ccgttcacgt agaggagccg ctggtcaccg acctcgcgga cggcgtggct
    13621 gacccccggc gactcgatcg ggatcgtgcc gacgtactcc atgttcgccg aggcgatcgc
    13681 acccggcagc tggaccaggc cggccgggtc cgcctgggcc ggtgccaccg caccggtgcc
    13741 ccacgccacc gcagccagcg cggccgtcgc cacgatcgaa ccccaacgca acgacctgga
    13801 cacctgcaac gctcctcgcc ccgccgaggc ccgcacggtg ctcgcgcggc gacccacctg
    13861 cgacggccga cggtgcgcac ttcgacggat cggcgccggc ttcctcccct c
//
LOCUS       DATISU010000200         7156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_661432, whole genome shotgun sequence.
ACCESSION   DATISU010000200 DATISU010000000
VERSION     DATISU010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7156
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661432"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1253)
                     /locus_tag="VLR27_08280"
     CDS             complement(72..1253)
                     /locus_tag="VLR27_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HSP03482.1"
                     /translation="MAYLDHAATTPVRPEARDAMLPWLGDRTGNPSGAHRLARDARRA
                     IDDARDTFAEVTGFDPGDIVFTAGGTEADNLAVLGVLDASDRPGATAVCPASEHHAVL
                     EAVEHRHGRIVRVRADGQVDLDHLAELLDDTVALVSVMAVNNETGVIADLHAVLDLVD
                     EHAPGAMVHTDAVQALTWLDLPAATTSPSGRRVDLLSLSAHKFGGPQGVGVLAVRAGT
                     PLQPRLLGGGQERGRRSGTQNVAGIVGAAAAARVATDTRSETVARLGALRDRLADGLH
                     DAIDGLVETAVDPSTRDRSAKVAGSCHVCIPGIEAEALLVLLEDRDVFASAASSCASG
                     AQDPSHVLAAMGIPRDVAAGSLRLSLGWSSTDADVDAALDAVPAAVERLRAFSSSGTP
                     A"
     gene            complement(1301..2188)
                     /locus_tag="VLR27_08285"
     CDS             complement(1301..2188)
                     /locus_tag="VLR27_08285"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HSP03483.1"
                     /translation="MTATQPAPVPPGRDLVGEGIVTPEAVLLEFAVAGLGSRSLAFLI
                     DLGIRAALLWLVFLGTAAGGIVVDDTVLVVISIAAVFSALLVYPALTETVWNGRTPGK
                     MAMGLRVVTIEGAPVRFRHAAIRSALGIVDFLLGAGTLAILSALATRQSQRLGDLAAG
                     TIVIRERQVRTDSRPVAFSPPPGWEAYTAALDVSRLRGDAYVLVRAFLLRVHELEPAA
                     RRERAASLAERVAAAIDVPLHPGTDPEAFLVAVAAAHQARHGDRGAVPTASFVPPPAG
                     KVVPPPVVDTDQPPATWGS"
     gene            2213..3235
                     /locus_tag="VLR27_08290"
     CDS             2213..3235
                     /locus_tag="VLR27_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HSP03484.1"
                     /translation="MDLDRYITEHQPAWDRLGHLARRGRRGAHRLEPAELDELVQLYQ
                     RVSGHLSHVRAAYGDPSLTALLTRLVADANAAIYGTRASARTAVRDFVLVTFPGAVWR
                     IRHFVLAAAVLTLLPAFAVGAWIANSDAALDASAPEAVRAAYVEDDFEAYYSSEPASQ
                     FAAEVTFNNIQVSITAFAVGILACIPTAGILAFNGANVGLAGGLFHAVGEWERFWGLI
                     LPHGLLEISAVIVAGGAGLRLGWTLIDPGDRPRMQALTEEGRQAVLVVLGLVGAFVVA
                     GLIEGFVTGSPLPTLVRVGIGVLVEIAFITYIVRLGRAATRHPVDRSPPVGAIDGISS
                     PYTVAT"
     gene            complement(3217..3939)
                     /locus_tag="VLR27_08295"
     CDS             complement(3217..3939)
                     /locus_tag="VLR27_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HSP03485.1"
                     /translation="MSRLFEEIDRRDTPIGEISVRRRLEPTLQIDIYEVKLGDEHLMS
                     SIFTVAEEELARLGLAAVGPDVGPDGLDVVVGGLGLGYTARAALEDERVRSLHVVEKL
                     AAVIEWQEEGIFPDAEVLTGDARTRLVEADFFACVADGTPFSPGEPDRFHAVLLDVDH
                     TPRHVLHPSHAAFYEPAGLARMAERIHPGGVFALWSDDPPDDGFIASAEEVFATVAAH
                     EVWFPNFHTGGEAMNTVYVATV"
     gene            complement(3965..5737)
                     /locus_tag="VLR27_08300"
     CDS             complement(3965..5737)
                     /locus_tag="VLR27_08300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP03486.1"
                     /translation="MAGIREQLREPLTAITDVFRNPGLARVNLALGGSETGDWAYGVA
                     VSIYAYLEGGATTVGILGVVRFVSAAIASPLTSTLADRGDRKRIMVRTDLIRFVLVAA
                     ATLVVAVDGPALAVYAAAVLATLTSTAFRPAQASIIPQLARHPDELTAANVASSTIES
                     LASFLGPAVAGVLLAVAGLEVVFAFNAVTFLWSALVLARLEVPDAEEDTGSGTAPPEP
                     TPALVGAAEPGDLLVDSGVAVDLEPVAAVLADPVQPAKESFLTEASQGFRTIWRSPDL
                     RVVVGVYSAQTVVAGASLVFTVAVALDLLGLGESSVGLLDASLGVGGMVGAFVALVLA
                     QRGSLARDFGFGVFAWAAPLLLVAAVPELWAALVAMVLIGVANAVVDVNAFTVLQRLV
                     PDEVMGRVFGAMESALIGAMAIGSLAMPLLIVTVGLRPGLVIVGGVVCVLAVAAMPRL
                     RHVDAVALAPAGLDTLRGLSMLAPLPLRVLERLARSSEVHRFAPGTTIFAEGDDGDRF
                     YVIEEGEVAVSIGGEEVGRLVAGGCFGEIALLRDVPRTATVRAVTDVGCRSLSQDVFV
                     PAVTGHGGARAQAEQVVDGWLGSV"
     gene            complement(5791..7098)
                     /locus_tag="VLR27_08305"
     CDS             complement(5791..7098)
                     /locus_tag="VLR27_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSP03487.1"
                     /translation="MPVPTARLAVVAALAAVAAALVPGSVVGRLLVVNGVLLGLALVD
                     WLLAPRGTSISIERELPAVITLGATSTVTWRVRSTARRPVRIAVADELAPSLGAGTRR
                     FRGTVPAGGALTTSTSLAPARRGRFTPTELVWRVEGPLGLAARQGRRSVPASLRVHPR
                     FRSREEAELKIDQARILEVGMRSATGRGGGTDFDQLREYTVDDESRRIDWAATARSGR
                     PIVRTFRAERNQNVVVLFDNGRVMAGRVAGVPRAEFAMDAALLVTTVATRLGDRCGLL
                     TFDRTVRGVVAPAHGRDQVGRVTDALYELEPELAESDYRGAFNETLVRFRKRSLIVLL
                     TDLVEQAVGESLLPALPVLTRSHLVLVGAIQDPQVRAWADGPVRDADDAYRQAAAART
                     LRARARAAARLRAAGATVVDVPPDRLASELGDAYLEMKAAGRL"
BASE COUNT          988 a         2549 c         2791 g          828 t
ORIGIN      
        1 gtggccctgc tcctggagga gcgcggcggc gaccgaggag tcgacgccgc cggacatggc
       61 gaccaggacc ctcatgccgg ggtccccgag gacgagaacg cgcgcaggcg ctcgacggcg
      121 gcgggcaccg catcgagcgc ggcgtcgacg tcggcgtcgg tgctggacca gccgagcgag
      181 aggcgcaggc tgccggcggc gacgtcgcgg gggatgccca tggcggccag cacgtggctc
      241 gggtcttggg cacccgaggc gcaggaggag gcggccgagg cgaacacgtc gcggtcctcg
      301 agcagcacga gcagggcctc ggcctcgatg ccggggatgc acacgtggca gctgccggcg
      361 accttggcgc tgcggtcgcg ggtggacggg tcgacggcgg tctcgaccag accgtcgatg
      421 gcgtcgtgga ggccgtcggc caggcggtcg cggagcgcgc cgagccgggc gaccgtctcc
      481 gaccgggtgt cggtggcgac ccgggccgcc gcggcggcgc cgacgatgcc cgcgacgttc
      541 tgggtgcccg agcggcgtcc gcgctcctgg ccgccgccga gcaggcgggg ctgcagcggc
      601 gtgccggctc ggacggcgag gacgccgaca ccctggggtc cgccgaactt gtgggctgac
      661 agcgacagca ggtcgacccg tcggccggac ggcgaggtcg tggcggcggg caggtcgagc
      721 caggtgagcg cctgcaccgc gtcggtgtgc accatcgcgc ccggcgcgtg ctcgtcgacg
      781 aggtcgagca cggcgtgcag gtcggcgatg acgccggtct cgttgttgac cgccatcacc
      841 gacacgaggg ccacggtgtc gtcgagcagc tcggcgaggt ggtcgaggtc gacctggccg
      901 tcggcccgga cgcgcacgat gcggccgtgg cggtgctcga ccgcctcgag cacggcgtgg
      961 tgctcgctgg ccgggcagac cgcggtggcg ccggggcggt cgctggcgtc gaggacgccc
     1021 agcacggcca ggttgtcggc ctcggtgccg ccggcggtga agacgatgtc gcccgggtcg
     1081 aagccggtga cctcggcgaa ggtgtcgcga gcgtcgtcga tggcccggcg ggcgtcgcgg
     1141 gcgaggcggt gcgcgccgga cgggttgccg gtgcggtcgc cgagccacgg gagcatcgcg
     1201 tcgcgcgcct cggggcgcac gggggtcgtg gcggcgtggt cgaggtaggc caccgcccca
     1261 ggctacgact ggcgaccgct acaggagcac tgccagttcc tcaagacccc caggtggcgg
     1321 ggggttggtc ggtgtcgacg acgggtggcg gcacgacctt gcctgccggc ggcggcacga
     1381 aggaggccgt cggcaccgcg ccccgatcgc cgtggcgggc ctggtgggcg gcggcgacgg
     1441 cgacgagaaa cgcctcggga tcggtgccgg ggtggagggg cacgtcgatg gcggcggcga
     1501 cgcgctcggc cagggacgcg gcgcgctcgc gacgggcggc cggctcgagc tcgtgcaccc
     1561 gcagcaggaa cgcccgcacg agcacgtagg cgtcgccacg cagccgggac acgtcgaggg
     1621 ccgccgtgta ggcctcccag cccggcgggg gcgagaacgc gaccgggcgg gagtcggtgc
     1681 gcacctggcg ctcgcggatg acgatggtgc cggcggccag gtcgccgagg cgctggctct
     1741 ggcgggtggc gagggcgctg aggatggcga gggtgccggc gccgagcagg aagtcgacga
     1801 tgccgagggc cgagcggatg gcggcgtggc ggaagcggac cggcgcgccc tcgatggtga
     1861 ccacccgcag gcccatcgcc atcttcccgg gcgtgcggcc gttccacacc gtctcggtca
     1921 gcgccgggta gacgagcagc gccgagaaca cggccgcgat ggagatcacc acgagcacgg
     1981 tgtcgtcgac gacgatgccg cccgctgccg tgccgaggaa cacgagccac agcagcgcgg
     2041 cacggatgcc gaggtcgatg aggaaggcca gcgagcggct gccgaggccg gccacggcga
     2101 actcgagcag caccgcctcg ggcgtgacga tgccctcccc gaccaggtcg cggcccggtg
     2161 gcaccggcgc gggctgggtg gcggtcacgc ctcggtacgg taccggccac ctgtggacct
     2221 cgaccgctac atcaccgagc accagccggc ctgggatcgc ctggggcacc tggcccggcg
     2281 gggcaggcgc ggggcccacc gcctggagcc ggccgagctc gacgagctgg tgcagctcta
     2341 ccagcgggtc tcgggccacc tctcccacgt ccgggccgcg tacggcgatc cctcgctcac
     2401 cgcgctgctc acccgcctcg tcgccgacgc caacgccgcc atctacggga cccgcgcgtc
     2461 ggcccgcacc gcggtgcggg acttcgtgct cgtcacgttc cccggcgcgg tctggcgcat
     2521 ccgccacttc gtcctggccg ccgcggtgct gaccctgctc ccggcgttcg cggtcggcgc
     2581 ctggatcgcc aacagcgacg cggccctcga tgcgtcggcc cccgaggcgg tgcgcgccgc
     2641 gtacgtcgag gacgacttcg aggcgtacta ctcctccgag ccggccagcc agttcgccgc
     2701 cgaggtgacg ttcaacaaca tccaggtgtc gatcaccgcg ttcgccgtcg gcatcctggc
     2761 ctgcatcccg accgccggga tcctggcgtt caacggcgcc aacgtcggcc tggccggtgg
     2821 cctgttccac gccgtcggcg agtgggagcg gttctggggc ctgatcctcc cccacggcct
     2881 gctcgagatc agcgccgtca tcgtcgccgg cggcgccggc ctgcgcctgg gctggacgct
     2941 gatcgaccca ggcgaccggc cccgcatgca ggccctcacc gaggagggcc gtcaggcggt
     3001 gctcgtggtg ctcggcctcg tcggcgcgtt cgtggtcgcc gggctcatcg agggcttcgt
     3061 caccggctcc cccctgccga cgctggtgcg ggtgggcatc ggcgtcctgg tcgagatcgc
     3121 cttcatcacc tacatcgtcc gtctcggccg ggccgccacc cgacatcccg tggatcgatc
     3181 accaccggtg ggcgcgatcg acgggatttc gagcccctac acggtggcga cgtagacggt
     3241 gttcatggcc tcgccgccgg tgtggaagtt ggggaaccac acctcgtggg cggcgacggt
     3301 ggcgaagacc tcctcggccg aggcgatgaa gccatcgtcg ggcgggtcgt cggaccacag
     3361 ggcgaacacg ccgccggggt ggatgcgctc ggccatgcgg gcgaggccgg ctggctcgta
     3421 gaaggcggcg tggctggggt gcaggacgtg gcggggggtg tggtccacgt cgaggagcac
     3481 ggcgtggaac cggtcgggct cacccgggga gaacggggtg ccgtcggcga cgcaggcgaa
     3541 gaagtcggcc tcgaccaggc gggtgcgggc gtcgccggtg aggacctcgg cgtccgggaa
     3601 gatgccctcc tcctgccact cgatgacggc ggcgagcttc tcgaccacgt ggaggctccg
     3661 gacccgctcg tcctcgaggg cggcgcgcgc cgtgtagccg agcccgagcc cgccgacgac
     3721 cacgtcgagg ccgtcgggcc ccacgtccgg accgacggcg gcgaggccga ggcgggccag
     3781 ctcctcctcg gcgacggtga agatgctcga catcaggtgc tcgtcgccca gcttcacctc
     3841 gtagatgtcg atctggagcg tgggctccag gcgccggcgg acgctgatct ccccgatggg
     3901 ggtgtcgcgc cggtcgatct cctcgaagag cctgctcacg gcggtcatcc tcacacgccg
     3961 gggatcagac cgagccgagc cagccgtcca ccacctgctc ggcctgggcc cgggcaccgc
     4021 cgtgcccggt gaccgcgggg acgaagacgt cctgggacag cgagcggcac ccgacgtcgg
     4081 tgacggcccg gacggtggcg gtgcggggca cgtcccgcag cagggcgatc tcgccgaagc
     4141 acccgccggc gaccagccga ccgacctcct cgccgccgat cgagaccgcc acctcgccct
     4201 cctcgatcac gtagaagcgg tcaccgtcgt cgccctcggc gaagatcgtg gtgccggggg
     4261 cgaagcggtg gacctcggag ctgcgggcca ggcgctcgag cacccgaagc ggcagcggcg
     4321 ccagcatcga caggccccgg agcgtgtcga gacccgccgg cgccaacgcc acggcatcga
     4381 cgtggcgcag gcggggcatg gcggccacgg cgaggacgca gacgaccccg cccacgatca
     4441 ccaggcccgg tcgcaggccg accgtgacga tgaggagcgg catggcgagc gagccgatgg
     4501 ccatcgcccc gatgagggcg ctctccatgg cgccgaacac ccgtcccatc acctcgtcgg
     4561 ggacgaggcg ctggagcacg gtgaaggcgt tgacgtcgac gacggcgttg gcgaccccga
     4621 tgaggaccat ggcgaccagg gccgcccaca gctccgggac ggcggcgacg agcagcagcg
     4681 gggccgccca ggcgaacacg ccgaagccga agtcgcgggc caggctgccg cgctgggcga
     4741 gcaccagcgc cacgaaggca ccgaccatcc caccgacccc gagcgaggcg tcgaggaggc
     4801 cgacggagga ttcgccgagc ccgagcaggt cgagggcgac cgccacggtg aagaccagcg
     4861 acgccccggc gaccaccgtc tgggccgagt agacgccgac caccacccgg aggtcggggc
     4921 tgcgccagat ggtgcggaac ccctggctgg cctcggtgag gaacgactcc ttcgccggct
     4981 ggaccgggtc ggcgagcacg gccgcgaccg gttcgaggtc gaccgccaca ccggagtcga
     5041 cgaggaggtc gccgggttcg gctgcgccga cgagggcggg cgtcggctcc ggtggggccg
     5101 tgccggaacc ggtgtcctcc tcggcgtcgg ggacctcgag gcgggcgagc accagcgccg
     5161 accacaggaa ggtcacggcg ttgaaggcga agaccacctc gaggccggcc acggcgagca
     5221 gcacgccggc gacggcgggg ccgaggaacg acgccaggct ctcgatggtg ctcgaggcca
     5281 cgttggccgc ggtgagctcg tcggggtgcc gggcgagctg cgggatgatc gaggcctgcg
     5341 ccgggcggaa cgcggtcgac gtcaacgtgg cgagcacggc ggcggcgtag acggcgaggg
     5401 ccggtccgtc gaccgcgacc acgagggtcg cggcggcgac gagcacgaac cggatcagat
     5461 cggtgcgcac catgatccgc ttgcggtcgc cccggtcggc gagcgtcgag gtgagcggtg
     5521 acgcgatcgc ggcggacacg aaccggacga cgccgaggat gccgacggtc gtggcaccgc
     5581 cctcgaggta ggcgtagatg ctgacggcga cgccgtaggc ccagtcgccg gtctcggacc
     5641 cgccgagcgc gaggttcacc cgcgccaggc ccgggttgcg gaacacgtcc gtgatcgccg
     5701 tcaacggctc acggagctgc tcgcggatgc cagccactcg attcccctcg ccctcgcccg
     5761 tccctgcgcg tgacggtact cgtctgcggg ctacagccgc ccggctgcct tcatctccag
     5821 gtaggcgtcg ccgagctcgg aggcgagccg gtcgggcggc acgtcgacga cggtggcgcc
     5881 ggcggcacgg aggcgggcgg cggcccgggc ccgggctcgc agcgtgcggg cggccgcggc
     5941 ctggcggtag gcgtcgtcgg cgtcgcgcac cgggccatcg gcccacgccc ggacctgtgg
     6001 gtcctggatg gcccccacca gcaccaggtg cgagcgggtc agcaccggca gcgccggcag
     6061 cagcgactcc cccaccgcct gctccaccag gtcggtgagc agcacgatga gcgaccgctt
     6121 gcggaaccgg accagggtct cgttgaacgc accccggtag tcgctctcgg ccagctcggg
     6181 ctcgagctcg tagagcgcat cggtgacccg gccgacctgg tcccgaccgt gcgccggagc
     6241 gaccacgccg cggaccgtgc ggtcgaaggt gagcaacccg cagcggtcgc cgaggcgcgt
     6301 ggccaccgtg gtgacgagca gggcggcgtc catggcgaac tcggcgcggg ggacgccggc
     6361 gacccggccg gccatcaccc gcccgttgtc gaacagcacc acgacgttct ggttgcgttc
     6421 ggcccggaag gtccgcacga tcggccggcc ggagcgggcc gtcgccgccc agtcgatccg
     6481 gcgggactcg tcgtcgacgg tgtactcccg gagctggtcg aaatcggtgc cgccgccccg
     6541 acccgtggcc gagcgcatgc ccacctcgag gatccgggcc tggtcgatct tcagctcggc
     6601 ctcctcccgg gagcggaacc gggggtggac ccgcagcgag gccggcaccg agcggcggcc
     6661 ctgccgagcg gccaggccga gggggccctc cacccgccac accagctcgg tcggggtgaa
     6721 ccgaccccgg cgggccggtg ccagcgacgt ggacgtggtg agcgcgccac cggccgggac
     6781 cgtgccgcgg aaccgccggg tgcccgcacc gaggctcggc gccagctcgt cggccaccgc
     6841 gatccggacc ggccgccggg cggtgctgcg cacccgccac gtgaccgtcg acgtggcacc
     6901 gagggtgatc acggccggga gctcgcgctc gatcgagatc gacgtgcccc ggggcgcgag
     6961 cagccagtcc accagggcga gcccgagcag caccccgttc accacgagca accggcccac
     7021 caccgacccg ggcaccagcg cggcggcgac cgccgcgagg gcggcgacga cggcgaggcg
     7081 ggcggtgggg acgggcaagg gttcagcgcg gcgccgggac gctggcgagg atgccgtcga
     7141 gcacggcgtc ggccgt
//
LOCUS       DATISU010000201        13447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_149701, whole genome shotgun sequence.
ACCESSION   DATISU010000201 DATISU010000000
VERSION     DATISU010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 13447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13447
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149701"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..712
                     /locus_tag="VLR27_08310"
     CDS             230..712
                     /locus_tag="VLR27_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03488.1"
                     /translation="MPLPRTVRPLAAAAASLLLLAGCGDDDDTGDDVSIEDVTEEPGL
                     DYGPLGQIVEGGDQYIGEEVTVTGEVTAQIDDRVFHIAAEPDTDGLLIVSQEPIVDEL
                     DSDDVVEVTGIVREKDADSFEGDFGMTYDERYTDFRGRHAIDATSVEVVGQADDPIDE
                     "
     gene            754..827
                     /locus_tag="VLR27_08315"
     tRNA            754..827
                     /locus_tag="VLR27_08315"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:788..790,aa:Lys,seq:ttt)
     gene            complement(905..1510)
                     /locus_tag="VLR27_08320"
     CDS             complement(905..1510)
                     /locus_tag="VLR27_08320"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSP03489.1"
                     /translation="MSDPSRLLVVRHGQSTWNAEGRWQGTENPPLSTLGVRQARHAAQ
                     QLGTFDAVVASDLERAFVTATILAEELGIGPVQTDADLRERSAGEYQGLTRHEIEERF
                     PGYLAEGLRPPDWEEDDVVLERAAGALGRVALAVGPGGTALVVTHGGVIHTLERALGL
                     RREQRLANLGGRWFTVGPGHFGGGDDVLLVTEDEVTVPNQL"
     gene            1544..1780
                     /locus_tag="VLR27_08325"
     CDS             1544..1780
                     /locus_tag="VLR27_08325"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSP03490.1"
                     /translation="MPTIRLFAGAREAAGTDRDTLDGATVREVLDAATARYGAGFAAV
                     LGTCRVWVNGEPAEPGDAVAEADEVAVLPPVSGG"
     gene            1782..2708
                     /locus_tag="VLR27_08330"
     CDS             1782..2708
                     /locus_tag="VLR27_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03491.1"
                     /translation="MARSTARPAEPTAKKAAKPTKKAAKKAGAKPKKKLTRAERAEAR
                     RREQVRKLRARSGNRYLVHYDIDGPRVRLGLAWFVGAVAALALGTVATALYFGVAFAA
                     AASHALRTWRARGAPVDPRVALIGTAVVVTAAAVHPRAMGVAVLLLAVGAMGMAAKDA
                     GADIRAVLRDTSLVLQTALPAAVAGGCFVLLVELELWAAISLVLLVSAYETGDFLIGS
                     GSTNAFEGPLAGGVAVLVVTLVVAALGFPPFDVGEAFAFGLLVAPGAFAGQILASAMM
                     PHARALAPALRRVDSLLLTAPLWYLGIDVLVV"
     gene            2781..3320
                     /locus_tag="VLR27_08335"
     CDS             2781..3320
                     /locus_tag="VLR27_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03492.1"
                     /translation="MRETLRTITADEYLGDLTALEMRELRARRATCQDVETGLSYLRR
                     MVQGRLDVAEAERARRADRTPTGDDDLEDLIARLPELLAHSTRSPGNGRLPSAFGTGD
                     IDDDLADELDDIVTTGRLSEPSELDDDELAAVVASLEAFERRVSALRRQLFDRIDAIE
                     VELTRRYRTGEASVDSLLS"
     gene            3343..3726
                     /locus_tag="VLR27_08340"
     CDS             3343..3726
                     /locus_tag="VLR27_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSP03493.1"
                     /translation="MSTAPPAILVVDDEPQVRTVVRSVLEADGYVVAEAADGPAALLL
                     LNSMNGPRGPHLVVLDVMMPGIDGVEVCRQIDGNRTKVVMLTARDDAETRAAAEEAGA
                     DRYLTKPFSAVELLDAVRELLADGS"
     gene            3732..4196
                     /locus_tag="VLR27_08345"
     CDS             3732..4196
                     /locus_tag="VLR27_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSP03494.1"
                     /translation="MGGPTDRPGAPAGPGADEPDDDEGGLRALGAFIRDQRREASLSM
                     RKLAETAGVSNPYLSQIERGLRKPSADILQQLARALSISSETLYVRAGILERERGDVD
                     VIGEILRDPSITVDQREALIRVYRSFRAETAAGAVPDQVDSTGQESSEGDRR"
     gene            4193..5422
                     /locus_tag="VLR27_08350"
     CDS             4193..5422
                     /locus_tag="VLR27_08350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSP03495.1"
                     /translation="MTATVQRLAVLSFHTSPLVQPGMGDSGGMNVYVRELVASLAQAG
                     IDCTVYTRAWAPDLPEVVDIEPGFRVVHVPAGAPDMPKEDLPSVIEEFTDGVLAHLAD
                     HPVDAVHANYWLSGVAGHRIKHTLDLPLVVTFHTLGRVKSVGGDPEPAHRIEAEASVI
                     GCADAILANTEAEVDELVRYYGADPARIEVVPPGVDHAFFSPGERHGARAALGLDDDH
                     PVILFVGRIQPLKGLDVSVRSLAQLERDDTILLAVGGTSGPAGEAHLADVVEEARELG
                     VLDRVRFVDAQPHHLLSTYYRAADVVHVPSRSESFGLVALEAAACGTPVVATAVGGLQ
                     TLVEHRRSGFLVESRDPACWASVTDHILSDPVLAERLAAGGVGIARNYTWSTTAGRLR
                     RVYADLAVRALVECAAA"
     gene            5437..5901
                     /locus_tag="VLR27_08355"
     CDS             5437..5901
                     /locus_tag="VLR27_08355"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HSP03496.1"
                     /translation="MQHASSPAELAALAAAIEDWLEVQREENPVVAAVERDEDPEVLR
                     WFVRVTGEEKDVFSIWFELGQRTLHYETYVMPAPEENHARFYEHLLRRNNRLMGAAFS
                     IGEEDAVFLAGALPNHAIDHDELDRILGSLYVWTEQFFKPALRIGFASRFPS"
     gene            6010..7191
                     /locus_tag="VLR27_08360"
     CDS             6010..7191
                     /locus_tag="VLR27_08360"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP03497.1"
                     /translation="MSRRPPRLLIFAITVTGILNNTLLGPSIPDILDDFGAGSSAAGL
                     IVAAGSLPGIVVAPVIGLLADRYGRRIVLTPCLAAFGTFGLATALAPTLEVMVITRLL
                     QGFGTAGLINLAVVLISDHWDGEARTALIGRNAAVLTLGLALAPALGGLVADALGWRV
                     ALLLYTIGLPMSALVWRVLDADQPTERETVGDQVRAARTAVGNPVVKASLGSGILVFV
                     LIFGGFLTTFPVHLEEVFGLSASARGLLIAVPAATASLIAFNLGRIRARWSMRTLLIT
                     AGVTYVAAFAGIASAGTIVVIVVACMLYGLGEGVFIPSLQDAVAGAAPPERRGAVLAV
                     WVGAARLGQTIGPLLAGGALALTSTTGVFGIGVVVALLLLAAQVLGPIGRSPVPSPAR
                     R"
     gene            7201..7992
                     /gene="proC"
                     /locus_tag="VLR27_08365"
     CDS             7201..7992
                     /gene="proC"
                     /locus_tag="VLR27_08365"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HSP03498.1"
                     /translation="MDRAALQIVGGGKMGEALLAGLLRSDPDLAVRVVETHGPRAEEL
                     RAAHPSLEVADVPGAAEGTLIAVKPHLVAEVAGAVGAADGGRVLSIAAGVTIAALEAA
                     LPPGAHVVRAMPNTPALVGAGAAAISAGGNADEADLAWAESILGAVGTVVRVPEHLLD
                     AVTGLSGSGPAYAFLVLDALIEGGVAAGLPRDVAEALAGQTLLGAATLFLHGDASPGE
                     LRAAVTSPGGTTAEGLRVLEQRAVRAALIDAVLAARDRAVALGAG"
     gene            8129..8860
                     /locus_tag="VLR27_08370"
     CDS             8129..8860
                     /locus_tag="VLR27_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03499.1"
                     /translation="MSNHRPDDVIPGTATRPTTPPTPAGGPPLASPPPDLGSPNWADE
                     QQGSSSGSQAKDSGREVAGHARSEIEDATQNASEQAKNVAETAKSEVRDVVDEARDAV
                     RQQADHQSEVAAGAIARFRDDLKAMSAGDSPRDATADYLQRAAGSLDNVVERLERDGI
                     EGALSGVRSFAQRSPGGFLLASAGAGFALGRILRNGDNPQQVQEQPTSGSSGQDREID
                     LTGDGPSRAAPAPRSESFAGGEMRR"
     gene            8857..9291
                     /locus_tag="VLR27_08375"
     CDS             8857..9291
                     /locus_tag="VLR27_08375"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HSP03500.1"
                     /translation="MTLAEARTNGRRPVRDAPSPETEIGALFGELSTEARLLVRQEVE
                     LAKAEIKESTQHATGVGAGFGAAALVGYLALAILAVAAALGLAELMPAGLAFLIVGLV
                     LALGAGIAFMVGKKNLKALNPVPHKTIETLKEDFSWLRARMS"
     gene            9267..9845
                     /locus_tag="VLR27_08380"
     CDS             9267..9845
                     /locus_tag="VLR27_08380"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HSP03501.1"
                     /translation="MATRTDELTAEIQTTRGRMDRTLDDIGDRAGPGRLTERLKGAVS
                     NTKDTVMGTAGDARDSVADSAGGASDAVKRQSQGNPIAAGLVAFGAGLLAGSVLPESR
                     TENKLAREVQSKAQAPIREHVQESASAVSDQVGERVGEAKEHIAEEGRHAKDEVASDV
                     SDRSDAVKGHAQGAAEDVKADVEDQARRQTGA"
     gene            9852..10874
                     /locus_tag="VLR27_08385"
     CDS             9852..10874
                     /locus_tag="VLR27_08385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HSP03502.1"
                     /translation="MATRTDEELIDLTEDGQPRRHPDGGDDRGRDAERPGDVPASGWK
                     DVALRVKGEVKEDQVSLLSAGVAFFLLLALFPALAALVSLYGLFADPDQVAQQVTDLT
                     GALPEEAQSLITDQLESITGQSQGIGVTLAVSIAAALWAASSGVKHLIGAVNAAYDEK
                     ESRGFLKLRGLSLGLTVGAIAFATIAIGVIAVLPALADELPFGSIGQTLVQIGSYVGL
                     AVGFALGLAVLYRYAPDRDAPRWQWVSWGAVIATVVWILGSVAFSVYVSNFGSYGETY
                     GSIGAVIVLMLWLVVTAFAIILGAEVNSELEAQTAKDSTVGRPQPMGQRDAVKADDLG
                     EPVARD"
     gene            10973..11173
                     /locus_tag="VLR27_08390"
     CDS             10973..11173
                     /locus_tag="VLR27_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP03503.1"
                     /translation="MTRGNGAVRPGFLTPREVAEALRVSDMTVYRLIKSGELRALRIG
                     KSFRISEEDFDAYLADRFTEAG"
     gene            11196..12077
                     /locus_tag="VLR27_08395"
     CDS             11196..12077
                     /locus_tag="VLR27_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HSP03504.1"
                     /translation="MSPLTYDTVSFLSDYGRGDEFVGIVHSILRSTAPGVAVIDITHD
                     IAPHDVRGGGLALARSAQYLCPGVVLAVVDPGVGTERRGIAIEVGDGQSVLVGPDNGV
                     LAPAVSMSGGATRAVSLTNTDYHLPAPGPTFDGRDVFAPVAAALCTGTELAELGDLVD
                     PSSLVPGLIPVMREEDGDLVCDVLWIDRFGNCQLNVDPDDIDEWGDRIGLRIGGELRV
                     ARRVDAYARIKEGEVGLVVDSYGLLSVAVDRRSAHVELALGTGQEVRLQRLDDDEGTG
                     GVTTPVNLGPRPQGGRP"
     gene            complement(12175..12957)
                     /locus_tag="VLR27_08400"
     CDS             complement(12175..12957)
                     /locus_tag="VLR27_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03505.1"
                     /translation="MRFDLVAYKESADRLDVDGIDFDAFRDQPLAPEHLRCLRYMHDV
                     ENHTSCYLRNLLNTRAHHDPEITTFLTLWNHEELWHGEALARVLAAHDEVGGVPRVAS
                     MRQRLGWRITASPLLWMAFSAMTPHFLAVHMTFGVVNEWTTQAGYVRLAAEADHPVLD
                     ELLRRIMRQEGTHIAFYRSHAVERLEASTQARRTTRRTVRALWSPVGASVMPEAETQH
                     LVRTLFSGEQGRAIAERIDRRVDALPGLDGLDLMAGTLARYG"
     gene            complement(13003..>13447)
                     /locus_tag="VLR27_08405"
     CDS             complement(13003..>13447)
                     /locus_tag="VLR27_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03506.1"
                     /translation="HSSIRDRDLFLAKFLSAFLPAVAVSWLGFLAFVIMANGVAWPVM
                     GRIFVPTRLWGVMILWVAPAVATLGLAVMVRVSARTHTAQEANQLGGAVILPLIFLAV
                     GQSTGLLLVTLPIAVGIGAVIWAIALLLLRGGVKRFTRDALVAQV"
BASE COUNT         1818 a         5120 c         4687 g         1822 t
ORIGIN      
        1 gtgctcgtcg accgggacgg cacctacatc gtccccaccg gtgcgacccg cctcgccgcc
       61 ggcgacaacg tcctcctgct cgccgacgac ggcaccttcc gcgacgcccg ggaccggctc
      121 actgcgcccg acgaggacgc ccctggcgct cggtgagacc ggggtcctga ctgcggcggc
      181 gacgccacgg gtagcgaccg ggggagacca gccacccagg agccacccca tgccactgcc
      241 ccgaaccgtc cgtcccctcg ccgcggctgc cgccagcctg ctgctgctcg ccggctgcgg
      301 agacgatgac gacaccggcg acgacgtgag catcgaggac gtcaccgagg aacccggcct
      361 cgactacggc cccctcggcc agatcgtcga gggcggcgac cagtacatcg gcgaggaggt
      421 gaccgtcacc ggcgaggtca ccgcccagat cgacgaccgg gtcttccaca tcgccgccga
      481 gcccgacacc gacggcctgc tcatcgtcag ccaggagccc atcgtcgacg agctcgacag
      541 cgacgacgtg gtcgaggtca cgggcatcgt acgggagaag gacgccgact ccttcgaagg
      601 ggacttcggc atgacctacg acgagcggta caccgacttc cgcggccgcc acgccatcga
      661 cgccacctcc gtcgaggtgg tcggccaggc cgacgacccg atcgacgagt gaccccgacc
      721 ggccggctcc ccgggctccg gtacggtgac cgggccctcg tagccaagct ggtcaaggca
      781 gcggactttt aatccgaaga tcgtgggttc gagtcccacc gggggcacgg cagccctgcg
      841 ggctgcatgg agttgctcgg ctgcgcctca caactccggc ggtgcgcatc ggctggccct
      901 tgcttcagag ctggttgggg accgtgacct cgtcctcggt cacgagcagg acgtcgtcgc
      961 cgccgccgaa gtggccggga ccgacggtga accaccggcc gccgaggttg gccaggcgct
     1021 gctcgcgccg caggccgagg gcgcgttcca gggtgtggat gaccccgccg tgggtcacca
     1081 ccagcgcggt gccgcctggc ccgaccgcca gggccacccg gcccagcgcg cccgcggccc
     1141 gctcgagcac cacgtcgtcc tcctcccagt cgggcgggcg gagcccctcg gccaggtagc
     1201 ccgggaagcg ctcctcgatc tcgtgccggg tgaggccctg gtactcgccg gccgagcgct
     1261 cccgcaggtc ggcgtcggtc tgcaccggcc cgatgccgag ctcctcggcc aggatcgtcg
     1321 cggtcacgaa cgcccgctcc aggtcggacg cgaccacggc gtcgaacgtg ccgagctgct
     1381 gggcggcgtg ccgggcctgg cggaccccga gggtggacag cggcgggttc tcggtgccct
     1441 gccagcggcc ctccgcgttc cacgtcgact ggccgtggcg gacgacgagg aggcgggacg
     1501 gatcggacat cggagcgacg gtagcccggt agcctcccgg tcgatgccga cgatccgcct
     1561 gttcgccggg gcacgggagg ccgccgggac cgatcgggac accctcgacg gtgccacggt
     1621 ccgcgaggtc ctcgatgccg ccacggcgcg gtacggcgcg gggttcgcgg cggtcctcgg
     1681 cacctgccgc gtctgggtca atggcgaacc cgccgaaccc ggcgacgccg tggccgaggc
     1741 cgacgaggtc gccgtcctgc ccccggtctc gggcggctga ggtggctcgc agcaccgctc
     1801 ggcccgcaga gccgaccgcg aagaaggcgg ccaagccgac caagaaggcc gccaagaagg
     1861 ccggggccaa gcccaagaag aagctgaccc gggccgagcg ggccgaggcc cgccggcgcg
     1921 agcaggtccg caagctgcgg gcccggtccg gcaaccgcta cctcgtccac tacgacatcg
     1981 acggaccgcg cgtccgcctc ggcctggcct ggttcgtcgg ggccgtcgcc gccctcgccc
     2041 tcggcacggt cgccaccgcg ctctacttcg gcgtcgcctt cgccgcggcg gcgtcgcacg
     2101 ccctgcgcac ctggcgggcc cgcggcgcgc cggtcgatcc gcgcgtcgcc ctcatcggca
     2161 ccgccgtggt cgtcaccgcc gctgccgtgc acccccgcgc catgggcgtc gcggtgctgc
     2221 tgctggccgt cggcgcgatg gggatggccg ccaaggacgc cggcgccgac atccgtgccg
     2281 tcctccgcga caccagcctg gtgctgcaga cggcgctgcc ggccgcggtc gccggcgggt
     2341 gcttcgtgct cctcgtcgag ctcgagctgt gggccgccat ctccctcgtg ctgctcgtgt
     2401 cggcctacga gactggcgac ttcctcatcg gctcggggtc gaccaacgcg ttcgagggcc
     2461 ccctcgccgg cggggtcgcg gtgctcgtgg tcacgctggt cgtcgccgcc ctcggcttcc
     2521 cgcccttcga cgtcggcgag gccttcgcct tcgggttgct cgtcgccccc ggtgcgttcg
     2581 ccgggcagat cctggcgtcg gcgatgatgc cccacgcgcg tgccctcgcc ccggcgctcc
     2641 gacgggtcga cagcctgctg ctcaccgcac cgctctggta cctgggcatc gacgtcctcg
     2701 tcgtctgacg ggccggcgct cccaggtggt ggggtagcct cggcttcgag cccgagcggg
     2761 cagaaccccg ggagcacacg ttgcgcgaga ccctgcgaac catcactgcc gacgagtacc
     2821 tcggcgacct cacggcgctc gagatgcgag agctgcgggc acggcgcgcc acgtgccagg
     2881 acgtcgagac cggcctgtcg tacctgcgcc gcatggtcca gggccgcctc gacgtggccg
     2941 aggccgagcg ggcccgtcgg gccgatcgca cccccaccgg cgacgacgac ctcgaggacc
     3001 tcatcgcccg cctccccgag ctgctggccc acagcacccg ctcgcccggc aacggccggc
     3061 tgccgtccgc gttcggcacc ggcgacatcg acgacgacct ggccgacgag ctcgacgaca
     3121 tcgtcaccac cggtcgcctc tccgagccga gcgagctcga cgacgacgag ctcgccgccg
     3181 tggtcgcgtc gctcgaggcg ttcgagcggc gggtgtcggc cctgcgccgg cagctgttcg
     3241 accgcatcga cgccatcgag gtcgagctca cccgccgcta ccgcacgggc gaggcgtcgg
     3301 tcgacagcct gctgtcctga gcacctctgc ttgactgccg ccatgagcac cgcgcctccg
     3361 gcaatcctcg tggtcgacga cgagccccag gtccgcacgg tcgttcgctc cgtgctcgag
     3421 gccgacggct acgtcgtcgc cgaggcggct gacggccccg ccgccctcct gctgctcaac
     3481 agcatgaacg gccctcgggg cccgcacctg gtggtgctcg acgtgatgat gcccggcatc
     3541 gacggcgtgg aggtgtgccg ccagatcgac ggcaaccgca ccaaggtggt catgctcacc
     3601 gcacgcgacg acgccgagac ccgggccgcc gccgaggagg ccggcgccga ccggtacctc
     3661 accaagccgt tctccgcggt cgagctcctc gacgcggtgc gagagctgct cgccgacggc
     3721 agctgacccc cgtgggtggt cccaccgacc ggccgggcgc gcccgccggg ccaggcgccg
     3781 acgagccaga cgacgacgaa ggcggcctgc gggcgctcgg cgccttcatc cgggaccagc
     3841 gccgcgaggc cagcctgtcg atgcgcaagc tggccgagac cgccggcgtc tcgaacccgt
     3901 acctgagcca gatcgagcgc gggctgcgca agccctcagc cgacatcctc cagcagctcg
     3961 cccgggccct ttcgatctcg tcggagaccc tctacgtccg ggcaggcatc ctcgagcgcg
     4021 aacgtgggga tgtcgatgtg atcggggaga tcctgcgaga cccgtcgatc accgtggacc
     4081 agcgcgaggc gctcatccgc gtctaccggt cctttcgcgc cgagaccgct gccggggcag
     4141 tacctgacca agttgattcg acaggtcagg aatcatccga gggggatcgg cgttgactgc
     4201 caccgtgcag cgtctcgccg tgctctcgtt ccacacgtcg cccctcgtgc agccggggat
     4261 gggcgacagc ggaggcatga acgtctacgt gcgcgagctg gtcgcgtccc tcgcccaggc
     4321 cgggatcgac tgcaccgtgt acacccgggc atgggcgccg gacctgcctg aggtcgtcga
     4381 catcgagccc ggcttccggg tcgtgcacgt gccggccggc gcccccgaca tgcccaagga
     4441 ggacctcccc tcggtgatcg aggagttcac cgacggcgtg ctcgcccacc tcgccgacca
     4501 ccccgtcgac gccgtgcacg ccaactactg gctgtcgggc gtggccggcc accgcatcaa
     4561 gcacaccctc gacctgccgc tggtcgtgac gttccacacc ctgggccggg tgaagtccgt
     4621 cggcggcgac cccgagcccg cccaccgcat cgaggccgag gcctcggtca tcgggtgcgc
     4681 cgatgccatc ctcgccaaca ccgaggccga ggtcgacgag ctggtccgct actacggcgc
     4741 cgatccggcc cgcatcgagg tcgtcccgcc cggcgtcgac cacgccttct tcagccccgg
     4801 cgagcggcac ggggcgcgcg ccgccctcgg cctcgacgac gaccatccgg tgatcctctt
     4861 cgtgggacgc atccagcccc tgaaggggct cgacgtctcg gtgcggtccc tcgcccagct
     4921 cgagcgcgac gacaccatcc tgctggcggt gggcggcacc agcgggccgg caggggaggc
     4981 ccacctcgcc gacgtggtcg aggaagcccg cgagctcggc gtcctcgacc gggtccggtt
     5041 cgtcgacgcc cagccccacc acctgctgtc cacctactac cgcgccgccg acgtcgtgca
     5101 cgtgccgagc cggtccgaga gcttcggcct cgtcgccctc gaagccgcgg cgtgcggcac
     5161 cccggtcgtg gccaccgcag tcggcgggct ccagaccctc gtcgagcacc gccgctccgg
     5221 cttcctggtc gagagccggg accccgcctg ctgggcctcg gtcaccgacc acatcctgtc
     5281 cgacccggtc ctcgccgagc gcctcgccgc cggcggcgtc ggcatcgccc gcaactacac
     5341 gtggtcgacc accgccggcc gcctgcgccg ggtctacgcc gacctggcgg tccgcgcgct
     5401 ggtggagtgc gcggcggcgt aaccgatact ggtcgggtgc agcacgcctc cagccccgcc
     5461 gagctcgccg ccctcgccgc cgccatcgag gactggctcg aggtgcagcg ggaggagaac
     5521 ccggtcgtcg ccgcggtcga gcgcgacgag gaccccgagg tcctgcgctg gttcgtgcga
     5581 gtgaccggcg aggagaagga cgtgttctcc atctggttcg agctgggtca gcgcacgctg
     5641 cactacgaga cctacgtgat gccggcgccg gaggagaacc acgcccgctt ctacgagcac
     5701 ctgctgcggc gcaacaaccg gctgatgggg gcggcgttct cgatcgggga ggaggacgcg
     5761 gtgttcctcg ccggcgccct gccgaaccac gccatcgacc acgacgagct cgaccgcatc
     5821 ctcggctcgc tctacgtgtg gaccgagcag ttcttcaagc cggcgctgcg gatcgggttc
     5881 gccagccgct tcccctcctg acttctcgtt gcatctcatg ccatcgtgtg ccagcatcgt
     5941 ggctgcgggt cggtgcccgg accggtcggg gaagccggac gtggcgccga cccgccaacc
     6001 ccgccggcgg tgagccgccg cccaccccgc ctgctgatct tcgccatcac ggtcacgggc
     6061 atcttgaaca acacgctgct cggcccgtcg atccccgaca tcctcgacga cttcggggcc
     6121 gggtcgtcgg cggcggggtt gatcgtggcc gccggctcgc tgcccggcat cgtcgtggca
     6181 ccggtcatcg gcctgctcgc cgaccgctac ggccgccgca tcgtgctcac cccctgcctt
     6241 gccgcgttcg gcacgttcgg gctcgccacc gccctggcgc cgacgctcga ggtcatggtc
     6301 atcacccggc tcctgcaggg cttcggcacc gccggcctca tcaacctcgc cgtggtgctc
     6361 atctccgacc actgggacgg cgaggcccgc accgcgctga tcggccgcaa cgccgccgtg
     6421 ctcaccctcg gcctggcgct ggccccggcg ctgggtgggc tcgtcgcgga cgccctcggc
     6481 tggcgggtcg ccctgctgct ctacacgatc ggcctgccga tgtcggcgct ggtgtggcgg
     6541 gtgctcgatg ccgaccagcc gaccgagcgg gagacggtcg gtgaccaggt gcgggcggcc
     6601 cgcaccgcgg tcgggaaccc ggtggtgaag gccagcctgg ggtcggggat cctcgtgttc
     6661 gtgctcatct tcggcgggtt cctcaccacg ttccccgtgc acctcgagga ggtgttcggt
     6721 ctgtccgcct cggcacgcgg gctgctcatc gcggtgccgg ccgcgacggc cagcctcatc
     6781 gcgttcaacc tcggccgcat ccgcgcccgc tggtcgatgc ggaccctgct gatcaccgcc
     6841 ggggtgacct acgtggccgc cttcgccggc atcgcctcgg ccggcaccat cgtcgtcatc
     6901 gtggtggcgt gcatgctcta cggcctgggt gagggcgtgt tcatcccgtc gctgcaggac
     6961 gccgtagcgg gtgccgcgcc ccccgagcgc cgcggcgcgg tgctcgccgt ctgggtgggg
     7021 gcggcgcgcc tcggccagac gatcggtccg ctgctggccg gcggagccct cgcactcacc
     7081 tcgaccaccg gcgtgttcgg gatcggggtc gtggtggcgc tgctcctgct ggccgcccag
     7141 gtgctcggcc cgatcggccg gagccccgtg ccgtcgccgg ctcgtcggta acgtcccgac
     7201 atggaccggg cagcactgca gatcgtcggc ggcggcaaga tgggcgaagc cctcctggcc
     7261 ggcctgctcc gatccgaccc ggacctcgcc gtgcgcgtcg tggagaccca cgggcctcgt
     7321 gccgaggagc tgcgcgccgc ccacccgtcg ctcgaggtcg ccgacgtgcc gggcgcggcc
     7381 gaggggaccc tcatcgccgt caagccccac ctcgtcgccg aggtcgccgg cgcggtcggc
     7441 gcggccgacg gcggacgggt cctgtcgatc gccgccgggg tcaccatcgc ggcgctcgag
     7501 gccgccctgc ccccgggggc gcacgtcgtc cgggccatgc ccaacacacc cgccctggtc
     7561 ggcgcgggtg ccgccgccat ctccgccggc ggcaacgccg acgaggccga cctggcctgg
     7621 gccgagtcca tcctgggcgc ggtcggcacc gtcgtgcggg tgcccgagca cctcctcgac
     7681 gccgtcaccg gcctgtccgg atccgggccg gcctacgcgt tcctcgtcct cgacgcgctc
     7741 atcgagggag gtgtggccgc cgggttgccg cgcgacgtcg ccgaggcgct ggccggccag
     7801 acgctcctcg gcgccgccac cctgttcctg cacggcgatg cctcgcccgg cgagctgcgc
     7861 gccgcggtga ccagcccggg tggcaccacg gccgagggcc tgcgggtgct ggagcagcgg
     7921 gcggtgcggg ctgcgctgat cgacgccgtg ctcgcggcac gcgaccgagc ggtcgcgctc
     7981 ggcgccggct gacgcgccgg gatcccacgc gaaccgggag gaaccccccg gaagaaccgg
     8041 cgaggtccgt gccattgggc gcgatccgtt ctgcgcggcg tgacgcctcg ggtagcgacc
     8101 gatggcacaa cgaccccagg agcgccacat gagcaaccac cgaccggacg acgtcatccc
     8161 cggcacggcc acgcggccga ccacgccacc cacccccgcc ggcggccccc cgctggcctc
     8221 cccgccgccc gacctcggct cgcccaactg ggcagacgag cagcagggca gcagctcggg
     8281 ctcgcaggcc aaggacagcg gccgtgaggt cgccggccac gcccgcagcg agatcgagga
     8341 cgccacccag aacgcttccg agcaggccaa gaacgtggcc gagacggcga agtccgaggt
     8401 ccgcgacgtc gtcgacgaag ctcgcgacgc agtccgccag caggccgacc accagtccga
     8461 ggtcgccgcc ggcgccatcg cccgcttccg cgacgacctg aaggccatga gcgccgggga
     8521 ctcgccccgc gacgccaccg ccgactacct ccagcgggcc gccggctccc tggacaacgt
     8581 ggtcgagcgc ctcgagcgag acggcatcga aggcgccctg tcgggcgtgc gcagcttcgc
     8641 ccagcgcagc cccggcggct tcctgctcgc ctcggccggc gctggcttcg ccctcggccg
     8701 catcctgcgc aacggggaca atccccagca ggtccaggag cagcccacct cgggctcgtc
     8761 gggccaggac cgggagatcg acctgaccgg tgacggtccc agccgcgccg ctcccgcccc
     8821 ccgcagcgag tccttcgccg gtggggagat gcgccgatga ccctcgccga agcacgcacc
     8881 aacggccgcc ggccggtgcg cgacgccccg tctcccgaga cggagatcgg cgcgctgttc
     8941 ggcgagctca gcacggaggc ccggctcctg gtccgccagg aggtcgagct ggccaaggcc
     9001 gagatcaagg agtcgaccca gcacgccacg ggggtcggcg ccggcttcgg cgccgccgcc
     9061 ctcgtcggct acctggcgct ggccatcctg gccgtcgccg ccgcgctcgg gctcgccgag
     9121 ctcatgccgg ccggcctcgc attcctcatc gtgggactcg tcctggcgct cggcgccggc
     9181 atcgcgttca tggtcgggaa gaagaacctg aaggcgctga acccggtgcc gcacaagacg
     9241 atcgaaaccc tcaaggagga cttctcatgg ctacgcgcac ggatgagctg accgcagaga
     9301 tccagaccac ccgcggtcgc atggaccgca ccctcgacga catcggtgat cgggccggcc
     9361 ccggccgcct caccgaacgc ctcaagggcg ccgtgtccaa caccaaggac accgtcatgg
     9421 gcaccgccgg cgacgcccgc gactccgtgg ccgattcggc cggcggcgcg agcgacgccg
     9481 tgaagcgcca gagccagggc aaccccatcg ccgccggtct ggtggccttc ggcgccggcc
     9541 tgctcgccgg ttccgtgctg cccgagagcc gcaccgagaa caagctcgcc cgcgaggtcc
     9601 agtcgaaggc ccaggccccc atccgcgagc acgtgcagga gtcggccagc gcggtctccg
     9661 accaggtcgg cgagcgcgtc ggcgaggcca aggagcacat cgccgaggag ggccgccacg
     9721 ccaaggacga ggtcgcgtcc gacgtcagcg accgctccga cgccgtcaag ggccacgccc
     9781 agggcgcagc ggaggacgtc aaggccgacg tcgaggacca ggcccgccgc cagaccggag
     9841 cctgagcacc gatggcgacc cgcaccgacg aagagctgat cgacctcacc gaggacggcc
     9901 agccccggcg gcatcccgac ggcggcgacg accgcggtcg ggatgccgag cgccccggcg
     9961 acgtaccggc cagcggctgg aaggacgtcg ccctgcgcgt caagggcgag gtcaaggagg
    10021 accaggtctc cctgctgtcg gcaggggtcg ccttcttcct cctgctcgcg ctgttcccgg
    10081 cgctggctgc gctggtctcg ctctacggcc tcttcgccga cccggaccag gtggcccagc
    10141 aggtcaccga cctcacgggt gcgctgcccg aggaggccca gagcctcatc accgaccagc
    10201 tcgagagcat caccgggcag tcgcagggca tcggggtcac cctcgccgtg agcatcgccg
    10261 ccgccctgtg ggccgcctcc tccggggtca agcacctcat cggtgcggtc aacgccgcct
    10321 acgacgagaa ggagagccgc gggttcctga agctgcgcgg cctctcgctc ggcctcaccg
    10381 tcggcgccat cgccttcgcc accatcgcca tcggcgtcat cgccgtgctc ccggcgctcg
    10441 ccgacgagct gccgttcggc tcgatcggcc agaccctggt ccagatcggt tcctacgtcg
    10501 gcctggcggt cggcttcgcc ctcggcctgg ccgtgctgta ccgctacgcg cccgaccggg
    10561 acgcaccccg ctggcagtgg gtgtcttggg gtgccgtgat cgccaccgtc gtgtggatcc
    10621 tcggctcggt cgccttctcg gtctacgtgt cgaacttcgg cagctacggg gagacctacg
    10681 ggagcatcgg tgccgtcatc gtgctcatgc tctggttggt ggtcaccgcg ttcgccatca
    10741 tcctcggcgc cgaggtcaac agcgagctcg aggcccagac cgccaaggac tccacggtgg
    10801 gccggcccca gccgatgggc cagcgcgacg cggtcaaggc cgacgacctc ggcgagcctg
    10861 tcgcccggga ctgaacccca ccagcaccat cccccactcc gcccccggta cgtgctagtg
    10921 gccacaggag ccacggtcgc ctagcctcgg tgccggagag aaggggtgtt ccatgacccg
    10981 tggcaatggt gctgtgcgac ccggcttcct caccccccgt gaggtcgccg aggcgctgcg
    11041 agtgtccgac atgaccgtct accggctgat caagtcgggc gagctgcgcg cactgcgcat
    11101 cggcaagtcg ttccggatca gcgaggagga cttcgacgcc tacctcgccg accggttcac
    11161 cgaagccggg tgaccgggcg tagcgtcgga ggggcatgtc cccgttgacc tacgacaccg
    11221 tctcgttcct ctccgactac ggccggggtg acgagttcgt gggcatcgtc cactcgatcc
    11281 tgcgctccac cgcgccgggc gtggccgtca tcgacatcac ccacgacatc gccccccacg
    11341 acgtgcgcgg cggtggcctg gccctggccc gcagcgccca gtacctgtgc cccggtgtcg
    11401 tgctggcggt cgtcgacccg ggcgtcggca ccgagcggcg cggcatcgcc atcgaggtcg
    11461 gcgacggcca gtcggtgctc gtcggaccgg acaacggcgt cctcgccccc gccgtgtcga
    11521 tgtccggcgg cgccacccgg gccgtgtcgc tcaccaacac cgactaccac ctgcccgcgc
    11581 ccgggccgac cttcgacggt cgcgacgtgt tcgcccccgt cgccgccgcg ctgtgcacgg
    11641 gcaccgagct cgccgagctg ggcgacctgg tggacccgag ctccctggtg cccggcctca
    11701 tccccgtgat gcgggaggag gacggcgacc tggtctgcga cgtgctctgg atcgaccggt
    11761 tcggcaactg ccagctcaac gtcgaccccg acgacatcga cgagtggggc gaccgcatcg
    11821 gcctgcgcat cggcggcgag ctgcgggtcg cccggcgggt cgacgcctac gcccggatca
    11881 aggagggcga ggtcggcctg gtcgtggact cctacgggct gctgtccgtg gcggtcgacc
    11941 gccgctccgc ccacgtcgag ctggcgctcg gcaccggcca ggaggtgcgc ttgcagcgcc
    12001 tcgacgacga cgaggggacc ggcggcgtca ccaccccggt caacctcgga ccacgccccc
    12061 agggaggcag gccatgaggc ccggaaccac catcatcatg gcgatcctgc tgctgttgat
    12121 catcggcgcc gccacgctcc agctcgtctt cctgacgtag cggccgcgac ggggtcagcc
    12181 gtagcgggcg agggtcccgg ccatcaggtc caggccgtcg aggccgggca gcgcgtccac
    12241 ccgtcggtcg atgcgctcgg cgatggcgcg accctgctcg ccggagaaca gggtgcgcac
    12301 gaggtgctgg gtctcggcct cgggcatcac cgacgcgccg accgggctcc acagcgcgcg
    12361 cacggtccgg cgcgtggtgc gtctggcctg cgtgctggcc tcgaggcgct cgaccgcgtg
    12421 cgaccggtag aacgcgatgt gggtgccctc ctggcgcatg atccgccgca gcagctcgtc
    12481 gagcacgggg tggtcggcct cggcggcgag gcggacgtag ccggcttggg tcgtccactc
    12541 gttcaccacc ccgaaggtca tgtggacggc gaggaagtgc ggcgtcatcg ccgagaacgc
    12601 catccacagc agggggctgg cggtgatgcg ccacccgagg cgctggcgca tggaggcgac
    12661 ccggggcacc ccgccgacct cgtcgtgggc ggcgaggacg cgcgccagcg cctcgccgtg
    12721 ccagagctcc tcgtggttcc acagcgtgag gaacgtggtg atctccgggt cgtggtgggc
    12781 tcgggtgttc agcaggttcc gcaggtagca cgacgtgtgg ttctcgacgt cgtgcatgta
    12841 gcgcaggcac cggaggtgct ccggggccag gggctggtcc cggaacgcgt cgaagtcgat
    12901 gccgtcgacg tccagccggt cggcggactc cttgtaggcg accaggtcga agcgcacgcc
    12961 ccttgttcgg aacgggtggt gtgcccggat tgcgtcgttc gctcagacct gggcgacgag
    13021 ggcatcccgg gtgaaccgct tgacgccgcc gcggagcagc agcagggcga tggcccagat
    13081 gacggcgccg atgccgacgg cgatcggcag cgtcaccagg agcagcccgg tggactggcc
    13141 gacggcgagg aagatcagcg gcaggatcac ggcgccgccg agctggttgg cctcctgggc
    13201 ggtgtgggtg cgggccgaga cgcgcaccat cacggccagg ccgagggtgg ccacggcggg
    13261 ggcgacccac aggatcatca cgccccacag gcgggtgggc acgaagatcc ggcccatcac
    13321 cggccaggcc acgccgttgg ccatgatcac gaaggccagg aagccgagcc acgacacggc
    13381 aaccgcgggc aggaacgccg acaggaactt ggccaggaac aggtcgcggt ctcggatcga
    13441 ggagtgc
//
LOCUS       DATISU010000202         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_615247, whole genome shotgun sequence.
ACCESSION   DATISU010000202 DATISU010000000
VERSION     DATISU010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615247"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VLR27_08410"
     CDS             <1..814
                     /locus_tag="VLR27_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSP03507.1"
                     /translation="IGQDEAVEAVAKAIRRSRAGLSDPHRPIGSFLFLGPTGVGKTEL
                     ARTLADFLFDDERAMVRIDMSEYMEKHSVSRLVGAPPGYVGHEEGGQLTELVRRRPYS
                     VILLDELEKAHPDVFNILLQVLDDGRLTDGQGRTVDFANTVLIMTSNMPGDPRDHLRP
                     EFINRIDDIVRFRELTEADLAPIVEIQLGHLHERLADRRISLEVTDAAKEKLAALGYD
                     PAFGARPLKRVIQREVADRIAGAILEGRLVDGDVATLDVSGDELTLETTQPV"
     gene            complement(900..1946)
                     /locus_tag="VLR27_08415"
     CDS             complement(900..1946)
                     /locus_tag="VLR27_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP03508.1"
                     /translation="MTDPFESLASPTEPQQPRPSFARTLRARLLDALELDATDAAPPI
                     DLPRRKPMPTTTTTPTATAVIPYLTATDAVGALAWYAQAFGAEEQFRVVGPDGLLGHA
                     EFRIGDAVFYLSDEHPALGVVSPTTLGGTPYAMHLTVTDVDEMFARAVAVGATAQREP
                     EDQPHGARHGTLLDPYGHRWMLSQPIEDVPLDEYAARAEDSPYRVEVAAGLRRDGQPY
                     VDGIWGSMTYTDPEVGIRFVTEVLGFEELVLVRDESGGIVHSEYRWPEGGIVGISAAD
                     EGNPFLPPPGHNGLYVVTADPHAVWERCRAAGVDVIREPEEPHYDPGGMGFSVRDPEG
                     NAWSFGSYAGGATE"
     gene            complement(1943..2482)
                     /locus_tag="VLR27_08420"
     CDS             complement(1943..2482)
                     /locus_tag="VLR27_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP03509.1"
                     /translation="MVSELPSTPDQRHALVDLYDRALPEVYGYLAARCGSASVAEDLT
                     SETFLAAVDAVRRGSVPHLSVAWLIGVARHKLVDHWRRYDRDQRLTAELDQPPASDDE
                     WDVHLDVLTAHETLATLGGHHRSALALRYLDGLPVREVAACLGRTEGATEVLLVRARA
                     AFRERYEEQRPTTAGEGER"
BASE COUNT          365 a          903 c          906 g          372 t
ORIGIN      
        1 catcggccag gacgaagccg tcgaggccgt ggccaaggcg atccgccgga gccgggcggg
       61 cctgtccgat ccgcaccgcc ccatcggctc gttcctgttc ctcggcccca cgggcgtcgg
      121 caagaccgag ctcgcccgca ccctcgccga cttcctcttc gacgacgaac gggccatggt
      181 ccgcatcgac atgagcgagt acatggagaa gcactccgtg tcccgcctcg tcggtgcgcc
      241 tcccggctac gtcggtcacg aggagggcgg ccagctcacc gagctcgtcc ggcgccggcc
      301 ctactcggtc atcctgctgg acgagctcga gaaggcccac cccgacgtct tcaacatcct
      361 gctgcaggtg ctcgacgacg ggcgcctcac cgacggtcag ggtcgcaccg tcgacttcgc
      421 caacacggtg ctgatcatga cgtcgaacat gccgggcgac ccgcgggacc acctgcgccc
      481 cgagttcatc aaccgcatcg acgacatcgt ccggttccgt gagctcaccg aagccgacct
      541 ggctccgatc gtggagatcc agctcggtca cctgcatgag cgcctcgccg accggcgcat
      601 ctccctcgag gtcaccgacg ccgccaagga gaagctcgcg gcgctgggtt acgacccggc
      661 tttcggcgct cgccccctca agcgggtcat ccagcgagag gtcgccgacc gcatcgccgg
      721 cgccatcctc gaaggtcgcc tcgtcgacgg tgacgtcgcc accctcgacg tctccggcga
      781 cgagctcacc ctcgagacca cccaacccgt gtaggaaact ggcagtcgaa tcgtcacacc
      841 cgtcgatccg tcgaaggatg gacagcactc ggtgtgacga ggcgactgcc agtttcgggt
      901 cactcggtgg cgcccccggc gtaggacccg aagctccagg cgttcccctc ggggtcgcgc
      961 acggagaagc ccatgccgcc ggggtcgtag tggggttcct cgggctcgcg gatgacgtcg
     1021 acgccggcgg cccggcagcg ctcccacacg gcgtgcgggt cggcggtgac gacgtacagc
     1081 ccgttgtgcc ccggcggggg gaggaacggg ttgccctcgt cggcggcgga gatgccgacg
     1141 atgccgccct ccggccagcg gtactcgctg tggacgatgc cgccgctctc gtcgcggacc
     1201 agcaccagct cctcgaaccc gagcacttcg gtgacgaagc ggatcccgac ctccgggtcc
     1261 gtgtaggtca tcgatcccca gatgccgtcg acgtagggct gtccgtcgcg acggaggccc
     1321 gcggcgacct cgacccggta cgggctgtcc tcggctcgag cggcgtactc gtccagcggg
     1381 acgtcctcga tcggctggga cagcatccag cgatggccgt agggatcgag gagggtgccg
     1441 tggcgcgcgc cgtggggctg atcctcgggc tcgcgctgcg ccgtggcgcc gactgcgacg
     1501 gcgcgggcga acatctcgtc cacgtcggtg acggtgaggt gcatcgcgta cggcgtgccg
     1561 ccgagggtgg tcggtgacac cacgcccagc gcagggtgct cgtcggagag gtagaagacg
     1621 gcgtcgccga tgcggaactc ggcgtgtccc aggaggccgt cgggaccgac cacccggaac
     1681 tgctcctcgg ccccgaaggc ctgcgcgtac caggccaggg cgccgacggc gtcggtggcg
     1741 gtgaggtagg ggatcacggc cgtggcggtc ggcgtggtcg tggtggtggg catgggcttc
     1801 ctcctcggaa ggtcgatcgg tggtgcggca tcggtggcgt cgagctcgag cgcgtcgagc
     1861 aaccgggcgc gcagggtgcg ggcgaagctg ggccggggct gctggggttc ggtcgggctg
     1921 gcgagggatt cgaacgggtc ggtcatcgct caccctctcc tgcggtggtc ggtcgctgct
     1981 cctcgtaccg ctcccggaac gccgcccggg cccggacgag caacacctcg gtggcgccct
     2041 cggtccgtcc gaggcacgcc gccacctcac gcacgggcag accgtcgagg tagcggagcg
     2101 cgagggcgct gcggtggtgg ccgcccaggg tcgccaacgt ctcgtgggcg gtgagcacgt
     2161 cgaggtggac gtcccactcg tcgtccgagg ctggtggctg gtcgagctcg gcggtgaggc
     2221 gctggtcccg gtcgtagcgc cgccagtgat cgacgagctt gtggcgggcc acgccgatca
     2281 accacgccac gctcaggtgc ggcaccgacc cgcgccgcac cgcgtccact gccgcgagga
     2341 aggtctcgga cgtgaggtcc tcggccaccg acgccgatcc gcagcgcgcc gccaggtagc
     2401 cgtacacctc gggcagcgcc cggtcgtaga ggtcgacgag ggcgtggcgc tggtccgggg
     2461 tcgaaggcag ctcgctcacc atcccctcca tcgtccggca gcgccgaagt cctacacccc
     2521 gatcttcacc gaggaacgca gccggg
//
LOCUS       DATISU010000203         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_848547, whole genome shotgun sequence.
ACCESSION   DATISU010000203 DATISU010000000
VERSION     DATISU010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848547"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1513
                     /locus_tag="VLR27_08425"
     CDS             <1..1513
                     /locus_tag="VLR27_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSP03510.1"
                     /translation="ASAIARDITERKAEESLHRGLLEAAPDAMVVVDDTGTIHLVNAQ
                     TEALFGYERSELVGQAVEVLVPEEYRNGHPGHRRGYFTEPRTRPMGQDLELTARRKDG
                     SVYPVEISLSSIETERGRLVSAAIRDVSERKAAEARFRGLVDAAPDAMVIVDPQGRIE
                     LVNAQADTMFGYERGELVGRPVEVLVPPRFRDQHPSHRSTYAHHARVRPMGAGLELFG
                     LRKDGSEFPVEISLSPLQTGDSVSISAAIRDVSVRKRAEAVQTAAFEREREASRRLRE
                     VDRLRSDFLSTVSHELRTPLTAIRGFADLLVEHWDATEGEQRRALVQRIAGAGLRLDR
                     LIEDLLDFTRLERGQLGIELRAQPLAELVDEALRHVAVSLERHEVDVDLEPDLVVLVD
                     EAAIARVLVNLLTNAAKFSPVGSTISIRAVTDGDRVAVSVIDRGSGVPAEEQDRIFER
                     FYRAQGATARRPGTGVGLAIVKEFTEAQGGEVHLRSAVGEGSEFTVRLRPART"
     gene            1580..1653
                     /locus_tag="VLR27_08430"
     tRNA            1580..1653
                     /locus_tag="VLR27_08430"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1614..1616,aa:Ala,seq:tgc)
     gene            complement(1695..2984)
                     /locus_tag="VLR27_08435"
     CDS             complement(1695..2984)
                     /locus_tag="VLR27_08435"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSP03511.1"
                     /translation="MVGGPTTLEEVAEALRNARDVCARLDPKAVPMHQVVDMFDSFVA
                     LERCAGGAVLRMAGRYEDAGQWKRNGAKSPEDDIARKTGTPTGKARRKLRTSKRLDEQ
                     PRTDEAVRRGRLSPDQADEVSDGAAASPEDEDELLDSAAKDPLHTLREKAAAARAKAD
                     KDREARRRRLRAQRCVRRWNDPDGMANLTLRLPGEDMAEIDAALKPRVDRAFADARNA
                     GRMERVEAYTADVVRDLLLGAAATDAPARRSQAVRPEKKVIALIDVPALNRGTVEDGE
                     TCEIAGVGPVSVSAIRALLSDAFLTVVFKDGIDIMNVTHLGRQVTAHQRTAMEARGGA
                     CERCGSRHLLDIDHNEGWTLTYDTRLEDLSWLCWHCHGLKTRHDLRLVGPVNERYLVD
                     RKTGKRWRPPPGEAPGDDPPPDATAGPAQPGLFTLAD"
BASE COUNT          389 a         1082 c         1100 g          440 t
ORIGIN      
        1 ggcctccgcc atcgcccggg acatcaccga gcgcaaggcc gaggagtcgc ttcaccgcgg
       61 gctgctcgag gccgcgcccg acgccatggt cgtggtcgac gacaccggca cgatccacct
      121 cgtcaacgcc cagaccgagg cgctgttcgg ctacgagcgc agcgagctgg tcggccaggc
      181 cgtggaggtc ctcgtcccgg aggagtaccg caacggccac cccggtcacc gccgcggcta
      241 cttcaccgag ccccgcaccc gccccatggg ccaggacctc gagctgacgg cccggcgcaa
      301 ggacgggtcg gtgtacccgg tggagatctc gctgtcgtcc atcgagaccg aacgaggacg
      361 cttggtctcg gcggccatcc gcgacgtgtc cgagcgcaag gcggccgaag cccggttccg
      421 gggcctcgtc gacgccgccc ccgacgccat ggtgatcgtc gacccgcagg gtcggatcga
      481 gctggtgaac gcccaggccg acacgatgtt cggctacgag cgcggcgagc tggtcggccg
      541 gccggtcgag gtgctggtgc cgccccgctt ccgcgaccag cacccgagcc accgcagcac
      601 ctatgcccac cacgcccggg tgcggccgat gggtgccggc ctcgagctgt tcggcctgcg
      661 caaggacggc agcgagttcc cggtggagat ctccctcagc ccgctgcaga ccggcgacag
      721 cgtctcgatc tccgctgcca tccgcgacgt cagcgtgcgc aagcgcgccg aagccgtgca
      781 gaccgcagcc ttcgagcggg agcgggaggc gtcgagacgc ctgcgggagg tggaccggct
      841 ccgatccgac ttcctgtcca ccgtctccca cgagctgcgc acgccgctca ccgccatccg
      901 gggcttcgcc gacctgctgg tggagcactg ggacgccacc gagggggagc agcggcgggc
      961 gctcgtgcag cgcatcgccg gtgccggcct ccgcctcgac cgcctgatcg aggacctgct
     1021 ggacttcacc cgcctcgagc gggggcagct cggcatcgag ctgcgggccc agccgctggc
     1081 cgagctcgtc gacgaggcgc tgcgccacgt cgccgtgtcc ctcgagcgcc acgaggtcga
     1141 cgtggacctc gagccggacc tcgtggtcct ggtcgacgag gccgccatcg cccgggtgct
     1201 cgtgaacctg ttgaccaacg cggcgaagtt ctcgcccgtg ggctccacga tctcgatccg
     1261 tgccgtcacc gacggcgacc gggtcgccgt gtcggtgatc gatcgcggct cgggcgtccc
     1321 ggccgaggag caggaccgga tcttcgagcg gttctaccgg gcgcagggcg ccacggcgcg
     1381 ccggcccggc accggcgtgg gtctggcgat cgtcaaggag ttcaccgagg cccagggcgg
     1441 cgaggtccac ctgcgctcgg ccgtgggcga gggcagcgag ttcaccgtcc gcctgcgccc
     1501 cgcccgcacc tgacggccga ccggcgccgg tccggtgtcg ctgccggggc gaccaccgcg
     1561 tagcctgtga actccccacg gggacgtagc tcagttggct agagcacctg ctttgcaagc
     1621 agggggtcgt gggttcgatt cccatcgtct ccacaaatcc gcgaccggcc cgcgccggtg
     1681 tccgaggcag ctcgtcagtc ggccagggtg aacaggccgg gctgggcggg cccggcggtg
     1741 gcatcgggcg gcgggtcgtc tcccggagcc tcgcccggcg gtggtcgcca tcgcttcccg
     1801 gtcttgcgat cgaccaggta ccgctcgttg accgggccca cgagccggag gtcgtggcgg
     1861 gtcttgaggc cgtggcagtg ccagcacagc caggagaggt cctcgagccg ggtgtcgtag
     1921 gtgagggtcc agccctcgtt gtggtcgatg tcgaggaggt ggcggctgcc gcagcgctcg
     1981 caggcgccgc cgcgggcctc catggcggtg cgctggtggg cagtgacctg ccggccgagg
     2041 tgggtgacgt tcatgatgtc gatgccgtcc ttgaacacca cggtcaggaa cgcatcggac
     2101 agcagggcgc ggatcgccga gaccgagacg gggccgacgc cggcgatctc gcaggtctcg
     2161 ccgtcttcga ccgtgccccg gttgagggcg gggacgtcga tgagggcgat gaccttcttc
     2221 tccgggcgca ccgcctgcga ccgacgggcc ggagcgtcgg tggccgcagc accgaggagg
     2281 aggtcccgca cgacatcggc ggtgtaggcc tccacccgct ccatccgtcc cgcgttccga
     2341 gcgtcggcga acgcccggtc gacccggggc ttgagggcgg catcgatctc ggccatgtcc
     2401 tcgccgggaa gtcgcagcgt caggttggcc atgccgtcgg ggtcgttcca gcgccgcacg
     2461 caccgctggg cgcggagacg gcgccgtcgt gcttcgcggt ccttgtcggc cttggcccgc
     2521 gccgcagcgg ccttctcccg cagcgtgtgc agcgggtcct tggccgccga gtcgagcagc
     2581 tcgtcctcgt cctcggggga ggcggccgcg ccgtcggaca cctcgtcggc ctggtccggc
     2641 gagaggcgcc cgcgccgcac ggcttcgtcg gtgcggggtt gctcgtcgag gcgcttgctg
     2701 gtgcgcagct tgcgccgagc cttgccggtg ggggtgccgg tcttgcgggc gatgtcgtcc
     2761 tcgggcgact tggcgccgtt gcgcttccac tgccccgcat cctcgtagcg gcccgccatg
     2821 cgcagcacgg ccccgcctgc gcaccgctcg agcgcgacga aggagtcgaa catgtcgacg
     2881 acctggtgca tggggacggc cttcgggtcg agacgagcgc agacgtcgcg agcgttccgc
     2941 agggcctctg cgacctcctc gagcgtggtg ggtcccccga ccatgcgacc acgatagcga
     3001 acaccagttc g
//
LOCUS       DATISU010000204         4745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_475877, whole genome shotgun sequence.
ACCESSION   DATISU010000204 DATISU010000000
VERSION     DATISU010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475877"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VLR27_08440"
     CDS             <1..431
                     /locus_tag="VLR27_08440"
                     /inference="COORDINATES: protein motif:HMM:NF021608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HSP03512.1"
                     /translation="ELQGLFGDPEVVLGAIQTDAQRALLPRLEALIAMIEGYVDHVMD
                     AIGTGLISSYGMITEAVRRRRVEADESDRFVARLFGLELGQATYDQGAAFVDGVVERE
                     GFDALDALWRGESFLPTPPEIEAPGLWLARIEIDTDTDTP"
     gene            complement(498..992)
                     /locus_tag="VLR27_08445"
     CDS             complement(498..992)
                     /locus_tag="VLR27_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HSP03513.1"
                     /translation="MSTRIVVLADTHVTRGSTRRLPDAVYEHLDAADRIWHAGDVLVP
                     ELLDELAGFAPVEAVLGNNDHELLGILPERTEETVDGVRIAMVHDSGATKGREGRMRR
                     WFPDADLVIFGHSHQPVDAEGVDGQRLLNPGSPTQRRRAPTHTIGIVTCDDGRLQTEI
                     VDLS"
     gene            complement(989..2323)
                     /locus_tag="VLR27_08450"
     CDS             complement(989..2323)
                     /locus_tag="VLR27_08450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP03514.1"
                     /translation="MASDGWRPLPAPRKGAEAFEATPFGRLARAHAVAVAGDTLVAIA
                     LAGTLFFSIDVDQARGEVFLYLALTMAPFAVVAPLVGPALDRAAGGRRWMVIGSSGLR
                     AFVCLLMVRDVDSLLLFPEAFALLVLAKSYHVAKSALVPTVVRSHEGLVEANSKLSLI
                     SGVVGFIAAVPGAALLRFGGSEWVLGLAAIVFAVGVFIAWGVPKVQIATEPESAEAKA
                     ELRGAGVLLAASAMALLRGIVGFLTFLLAFSLRTDGSPTWHFGVVLALSAVGSLVGAA
                     LAPALRRQTTEERIISYLLVVVAALAVVAAWLGALPGAALVAVTVGISAAGAKQAFDS
                     IVQRDAPDANRGRSFARFETRFQLVWVGGAVLGIIPMPMWVGFLLVAGVSSFAGLSYA
                     AGSRGMRDVVQRRVQRARRAASGDATTVAPSVPPSIATPPRPGDDPTIVEDA"
     gene            complement(2379..3068)
                     /locus_tag="VLR27_08455"
     CDS             complement(2379..3068)
                     /locus_tag="VLR27_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP03515.1"
                     /translation="MGELTVLVVEDEESFVEALTVGLAREGFRVQVAWDGAMALDMFD
                     AVRPDIVLLDVMLPKISGVDVCRQLRKRSSVPIIMVTAKGSEIDTVVGLEVGADDYVT
                     KPYRIRELVARMRAVIRRSADREDDLGTDALEVDEIALDPERHEVRIRGEQVQLPLKE
                     FELLHVLLANAGRVITREQLIDRVWGSDYVGDTKTLDVHVKRLRAKVEVDPANPERIV
                     TIRGLGYKLEA"
     gene            complement(3070..4311)
                     /locus_tag="VLR27_08460"
     CDS             complement(3070..4311)
                     /locus_tag="VLR27_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP03516.1"
                     /translation="MALAAAIPTVVLVLVLLLGLRTWRRTRQRVHLAATRLSPTGLAE
                     PGGLEQTLGQLERTVDAAVSQGGEATAAETRMSHALRAIDHGIVLFDESGEIAFRNAQ
                     AKIFLGARYGDAVVESSIDELATRALEGHDSVRTLELFGPPRRTLVLTADPLDDGHRS
                     IGALVMIEDITERRRVDAVRRDFVANISHELKTPVGGLSVLAETIVDEEDLSVIRRLA
                     QRMHHEAMRASRTIDDLLELSRIEAGEAALREPVSVHLVLAEAVERAANAAEHRGIGL
                     DLQEPSHRMQVRGDRRQLASAVFNLLDNAIKYSEPGSTVQVRAETDDGSARLIVADDG
                     IGIPRRDLERIFERFYRVDRARSRDTGGTGLGLAIVRHVASNHGGEITVESREGEGST
                     FSLVLPLSDGPVSVIDDEEEC"
     gene            complement(4326..>4745)
                     /locus_tag="VLR27_08465"
     CDS             complement(4326..>4745)
                     /locus_tag="VLR27_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSP03517.1"
                     /translation="VVEQALQGAALWDEVKDRLKESGMSLSGGQQQRLCIARAIATDP
                     EVLLMDEPCSALDPIATGRIEDLMVEIKERYTIIIVTHNMQQAARVSDRTAFFTTEVS
                     EESDRRTGLLVEFSDTDKIFTNPADERTENYVTGRFG"
BASE COUNT          681 a         1693 c         1730 g          641 t
ORIGIN      
        1 ccgagctcca gggcctgttc ggcgatcccg aggtcgtgct cggcgccatc cagaccgacg
       61 cccagcgcgc cctgctcccc cgcctcgagg ccctcatcgc catgatcgag ggctacgtcg
      121 accacgtcat ggacgccatc ggcaccgggc tgatctccag ctacggcatg atcaccgagg
      181 ccgtccgccg gcgccgggtc gaggccgacg agtccgaccg gttcgtggcc cggctgttcg
      241 gcctcgagct gggccaggcc acctacgacc agggcgccgc gttcgtcgac ggcgtggtcg
      301 agcgggaggg cttcgacgcc ctcgatgcgc tgtggcgggg cgagtcgttc ctgcccacgc
      361 cacccgagat cgaggcaccg ggcctctggc tcgcccgcat cgagatcgac accgacaccg
      421 acaccccctg accgcttctc cacgcgcaga acgaccgccc acggtcggat cgcgcgccgg
      481 gaagctggcg gccaggctca ggagaggtcg acgatctcgg tctgcaggcg gccgtcgtcg
      541 caggtgacga tgccgatcgt gtgggtcggc gcccgccggc gctgggtcgg tgacccgggg
      601 ttgagcagcc gctggccgtc cacgccctcg gcgtccaccg gttggtggct gtggccgaag
      661 atcaccaggt cggcgtcggg gaaccagcgg cgcatgcgcc cctcccggcc cttggtggcg
      721 ccgctgtcgt ggaccatggc gatgcggacg ccgtcgacgg tctcctcggt gcgctcgggc
      781 aggatgccga gcagctcgtg gtcgttgttg ccgagcaccg cctccaccgg cgcgaacccg
      841 gccagctcgt cgagcagctc ggggacgagc acgtcgccgg catgccagat gcggtcggcc
      901 gcgtcgaggt gctcgtagac ggcatcgggc aaccggcggg tcgagccgcg ggtcacgtgg
      961 gtgtcggcca ggaccacgat gcgggtgctc acgcgtcctc cacgatggtg ggatcgtccc
     1021 cgggtcgggg cggcgtggcg atcgacggcg gcacgctcgg cgccaccgtc gtcgcatccc
     1081 ccgacgcggc tcgccgggcc cgctggacgc ggcgctgcac gacgtctcgc atcccccggg
     1141 acccggcggc gtaggacagg ccggcgaagg acgagacgcc ggcgacgagc aggaagccga
     1201 cccacatcgg catggggatg atgccgagca ccgcgccgcc gacccagacc agctggaacc
     1261 gggtctcgaa gcgggcgaag gaccggcccc ggttggcgtc cggcgcgtcg cgctgcacga
     1321 tcgagtcgaa cgcctgcttg gcgccggccg cgctgatccc gacggtgacg gcgacgaggg
     1381 ccgcgccggg cagcgcgccg agccaggccg cgacgacggc cagggcagcg acgaccacca
     1441 gcaggtagct gatgatgcgc tcctcggtgg tctggcggcg cagcgcgggc gcgagggcgg
     1501 cgccgacgag cgagccgacc gccgacaggg ccaggacgac gccgaagtgc caggtcgggg
     1561 acccgtcggt gcgcagggag aaggcgagca ggaaggtgag gaagccgacg atgccccgca
     1621 gcagggccat cgccgaggcg gccagcagca cgccggcacc ccgcagctcg gccttggcct
     1681 cggcgctctc cggctcggtg gcgatctgga ccttcgggac gccccaggcg atgaacacgc
     1741 ccacggcgaa cacgatggca gcgaggccga gcacccactc gctcccaccg aagcgcagga
     1801 gcgccgctcc gggcaccgcc gcgatgaagc ccacgacgcc gctgatgagg gacagcttgg
     1861 agttggcctc cacgagcccc tcgtgggagc gcacgaccgt gggcaccagc gccgacttgg
     1921 ccacgtggta gctcttcgcc agcaccagga gcgcgaacgc ctcggggaac agcagcagcg
     1981 agtccacgtc gcgcaccatc agcaggcaca cgaaggcccg cagtccggac gagccgatca
     2041 ccatccaccg gcggccgccg gcggcccggt cgagggcggg gccgacgagg ggggcgacga
     2101 cggcgaaggg cgccatcgtg agcgccaggt agaggaacac ctcgccgcgg gcctggtcga
     2161 cgtcgatgga gaagaacagc gtccccgcca gcgcgatggc gaccagggtg tcgccggcga
     2221 cagcgacggc gtgggcccgg gccaggcggc cgaacggcgt ggcctcgaac gcctcggccc
     2281 ccttgcgggg tgccgggagg gggcgccagc cgtcggatgc catcgttcgc atcgtagggc
     2341 cggcctccgg gcgatccgac cgcgtggcgg ccgggtcctc aggcctccag cttgtagccg
     2401 aggccccgga tggtgacgat gcgctcgggg ttcgccgggt cgacctcgac cttggcccgc
     2461 agccgcttca catgcacgtc gagggtcttg gtgtcgccca cgtagtccga gccccacacc
     2521 cggtcgatga gctgctcgcg ggtgatgacg cggccggcgt tggccagcag gacgtgcagc
     2581 agctcgaact ccttcagcgg cagctggacc tgctcgcccc ggatgcggac ctcgtggcgc
     2641 tcggggtcga gcgcgatctc gtcgacctcg agcgcgtcgg tgccgaggtc gtcctcccgg
     2701 tcggcggagc gacggatcac ggcgcgcatg cgggccacca gctcccggat ccggtacggc
     2761 ttggtgacgt agtcgtcggc accgacctcg aggcccacga cggtgtcgat ctcggatccc
     2821 ttcgccgtga ccatgatgat cggcacgctg gagcgcttcc gcagctgccg acatacgtcg
     2881 acgccggaga tcttcggcag catgacgtcg agcaggacga tgtcggggcg caccgcgtcg
     2941 aacatgtcca gcgccatggc gccgtcccat gccacctgga cccggaaccc ctcccgggcg
     3001 agcccgacgg tgagggcctc cacgaaggac tcctcgtcct cgaccaccag cacggtcagc
     3061 tcgcccacgt cagcactcct cctcgtcgtc gatgaccgac accggcccgt cgctgagcgg
     3121 gagcaccagg ctgaaggtgg aaccctcgcc ctcccggctc tccaccgtga tctcgccgcc
     3181 gtggttggac gccacgtggc gcacgatggc caggccgagg ccggtgccac cggtgtcgcg
     3241 gctgcgggcc cggtcgaccc ggtagaagcg ctcgaagatg cgctcgaggt cgcgacgcgg
     3301 gatgccgatg ccgtcgtcgg ccacgatcag ccgggccgat ccgtcgtcgg tctcggcccg
     3361 cacctgcacg gtggagccgg gctcgctgta cttgatggcg ttgtcgagga ggttgaagac
     3421 ggccgatgcc agctggcggc ggtcgccccg gacctgcatg cggtgcgagg gctcctggag
     3481 gtcgaggccg atcccgcggt gctcggcggc gttcgccgcc cgctccacgg cctcggcgag
     3541 gaccaggtgc acggagacgg gttcccggag ggcggcctcg ccggcctcga tgcgggacag
     3601 ctcgagcagg tcgtcgatgg tccggctggc acgcatcgcc tcgtggtgca tgcgctgggc
     3661 cagccggcgg atcaccgaca ggtcctcctc gtccacgatc gtctcggcca gcacgctgag
     3721 cccaccgacg ggcgtcttga gctcgtggct gatgttggcg acgaagtccc ggcggaccgc
     3781 gtcgacgcgc cggcgctcgg tgatgtcctc gatcatcacc agcgccccga tgctgcggtg
     3841 gccgtcgtcg aggggatcgg ccgtgagcac cagcgtccga cggggcgggc cgaacagctc
     3901 gagggtgcga acggagtcgt ggccctccag ggcgcgggtg gccagctcgt cgatggagga
     3961 ctcgaccacg gcgtcgccgt agcgggcgcc gaggaagatc ttggcctggg cgttgcggaa
     4021 cgcgatctcc ccggactcgt cgaacagcac gatgccgtgg tcgatggcgc gcagggcgtg
     4081 cgacatgcgg gtctcggctg cggtggcctc gccgccctgg ctgaccgcgg cgtcgaccgt
     4141 gcgctcgagc tggccgagcg tctgctcgag cccgccgggc tcggcgagac cggtgggcga
     4201 cagcctcgtg gcggccaggt gcacgcgctg gcgggtgcgg cgccaggtgc gcaggccgag
     4261 cagcagcacg agcaccagca ccacggtcgg gatcgctgct gccagtgcca caggggctga
     4321 gtccatcacc cgaagcggcc ggtgacgtag ttctcggtgc gctcgtcggc ggggttggtg
     4381 aagatcttgt cggtgtcgga gaactcgacg agcaggccgg tgcgccggtc gctctcctcg
     4441 ctgacctcgg tggtgaagaa cgccgtgcgg tccgacaccc gcgccgcctg ctgcatgttg
     4501 tgggtcacga tgatgatcgt gtagcgctcc ttgatctcca ccatcagatc ctcgatccga
     4561 cccgtcgcga tgggatccag cgccgagcac ggctcgtcca tcaacagcac ctccggatcg
     4621 gtggcgatcg cccgcgcgat gcacagccgc tgctgctgcc cacccgacag gctcatcccc
     4681 gactccttca gccggtcctt cacctcgtcc cacagcgccg caccctgcag cgcctgctcg
     4741 acgac
//
LOCUS       DATISU010000205        11767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_475908, whole genome shotgun sequence.
ACCESSION   DATISU010000205 DATISU010000000
VERSION     DATISU010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 11767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11767
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475908"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VLR27_08470"
     CDS             complement(<1..597)
                     /locus_tag="VLR27_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03518.1"
                     /translation="MSPTVRRLLRLDRATIVLVTTVAVLATVVGITVASPPDAVRPAD
                     VDGRTWMSVPTGNGPGLVLLNGFSGLVEARARAGGDLPPSTRFAASGSSATLLAGPDG
                     VVVVDDGLHDARSLHVPTQGAVLVGDDVLVVDRDGAVSIQRPGKEPTTLHLDGDEAIG
                     EPLLDDDGVAWLLAEHAAGHRAWRVGHDLEPTTRDVPPT"
     gene            complement(712..1395)
                     /locus_tag="VLR27_08475"
     CDS             complement(712..1395)
                     /locus_tag="VLR27_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03519.1"
                     /translation="MTATTTTTTTAPTSRTAPPRGNGSARWAGWAGVGFATALIAGFG
                     LVAQVPAYDAPEADWVSWVENDNVTTVIGTMLLTAAGLLLMAFVTTLATLVRGAGIED
                     DPPITVLRGAGIMAGVTLVIAALVGGAMAAGVTFAPDFAAPGGELIRSLDQLSLGTML
                     VGTGWSMALAVALASWAARRTGTLPGWLSTGGFVVAALLLLSPMFLPFLLVPLWGLVT
                     GIVVLRAAH"
     gene            complement(1410..2276)
                     /locus_tag="VLR27_08480"
     CDS             complement(1410..2276)
                     /locus_tag="VLR27_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03520.1"
                     /translation="MTTAHRHPIARASAALAALVLATSVLAACGDDDDDTAEGSDDPT
                     AEAVSAPDEGAATDEEPASDEGASDDSFCEGFLGLEAAFAEAPEDEAEIPAFVAERID
                     PNLALIEGNEPADVAADVQTMTAAVTEVTSTGDFSAFETPEFLAASEAVYGSLDDSCD
                     VEVVEFSAIDYGYEGLPSTLPAGATSFVMSNDSEAGEAHEFSLVKLSDDTELSIEELL
                     EMPEAEAEQHIEAFGGGTFAPAGAASGTVTELSPGRWAYVCFIPVGTVGDTEGTGPPH
                     FVEGMAGELTVD"
     gene            complement(2273..2722)
                     /locus_tag="VLR27_08485"
     CDS             complement(2273..2722)
                     /locus_tag="VLR27_08485"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSP03521.1"
                     /translation="MTDTTPTARRARSVNAADWSRRLGHDQAQRRDGVSAILSIGGQR
                     PVDAEGRLMHEGDAIAQVALALDNLTAVVVAAGMTLADLAHLRIHATSRAALLDAQFV
                     VREHLVEHAVTTPITTVEVRGLADPGMEVEIDGLAVRTENRTEGNPT"
     gene            2835..3959
                     /locus_tag="VLR27_08490"
     CDS             2835..3959
                     /locus_tag="VLR27_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSP03522.1"
                     /translation="MGKALAIERVRSDVEVLARAGLDVSTFLAEVDASLVRAVPYEAM
                     CVALQDPATSMATATFKFGGISGRESHDLHWCEIEFGAADDTSFRSLTDRGVTAVGLH
                     HETGGDVARSVRMRDFMCHHFGFTDELRTVARVDDRDWAALAMMRADAANPFDDAEIA
                     LMSSLSGALATGIRAGVLARCADSSATTLEGPAVIIVGRDGSFTQVNPAATHWLAELA
                     SCDNSEGPVHTLAALIDGARRFARGEVDTLPRCRVRVPSGTWLVLNGSPLSGPDSPGT
                     DIVITIEQARPPEIVPLVVAAFALTPRERDVTQLVLQGVPSKEVASTLHISVHTVQDH
                     LKSIFEKADVRSRRELIARVFFDQYLPRFGDEVTTSGWFA"
     gene            complement(3987..5516)
                     /locus_tag="VLR27_08495"
     CDS             complement(3987..5516)
                     /locus_tag="VLR27_08495"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSP03523.1"
                     /translation="MSTEHLDVLVVGAGLSGIGAGHYLQAECPWASYAIFEARDAIGG
                     TWDLFRYPGIRSDSDMYTLGYSFRPWTGEKSIADGASILEYIRETARVEGIDRQIRFN
                     HRVVTADWSTEDARWHITAERTDPDRPDAAPERIELTAGFIFSCSGYYRYDHGYQPDF
                     AGMDDFAGQVIHPQHWPEDLDYAGKQVVVIGSGATAVTLVPSMADPGDGSGAAHVTML
                     QRSPSYVVSMPAKNPLARLLQRLLPTRLSGPIVKWMNALGSQGFFQLSQRFPGMVKKL
                     LRKRLEGLLPEGYDIDTHFTPSYDPWDQRVCLVPDGDLFRAIREGSVSVVTDHIDTFT
                     ETGIRLQSGRELAADIIVTATGLELLFIGGIEASVDGEPVDIASKLTYKGMMLEGVPN
                     LALAIGYTNASWTLKCDLTCDYVTRMLNHMRATGLRQATPVNADASVTPAPLLGLSSG
                     YIQRAADRFPKQGSRMPWQVHQSYLRDYRALKRSEVTDGVMEFSNPAPAVDPRPATLA
                     S"
     gene            complement(5611..6267)
                     /locus_tag="VLR27_08500"
     CDS             complement(5611..6267)
                     /locus_tag="VLR27_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HSP03524.1"
                     /translation="MDVTEAVRRRTSIRAFLDTPVDDDTVAELLELAARAPSGGNVQP
                     WRIYVIGADTMPKFLSFIEGRQMETPGYEIYPSGLWEPYRTNRFQLGEQMYETLGIPR
                     EDKAGRFGHLARNYRFFDAPVGLFCFIDRRMGPPQWSDLGMFLQTFMLLCTERGIDTC
                     PQEAWAVWSEAVAEFVGAPDDEMLFCGVAVGHADPEAPVNSLRSERMPLEEWATFVRP
                     "
     gene            complement(6267..7211)
                     /locus_tag="VLR27_08505"
     CDS             complement(6267..7211)
                     /locus_tag="VLR27_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03525.1"
                     /translation="MGLLDRFKKAPGPDAMTWEAVARLRVVAGVVGAEPVDADTVQIA
                     WADNPYPSTLSLAAAREGWTKANGFERIEIMDALIGAVAPPTGPPEGAEEPGVDPTPP
                     DVAPDVQVTGTQAPSDDPAPAPAGGTGWDAARDRLRVVVGCAGEHPAAITWPIAGVLE
                     ARVVLGGPEALPVDAGDAASWEVSADDVRAAALTNLAATDPGLDPVGPGQPAWVPTSP
                     ADHPPAWLAAPDRLLAACGLDQAVVLAPMPTELVVVDPSAHDLLSSILASTKSIVAGE
                     SRLLWPAPLLATSAGLSPWQPAPDHPCASLAAELGGSA"
     gene            complement(7265..7900)
                     /locus_tag="VLR27_08510"
     CDS             complement(7265..7900)
                     /locus_tag="VLR27_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03526.1"
                     /translation="MLDGHPRIRTFHARHGRITDVMRAALAEIGPRHDVAHRDPARPL
                     VLEVGCGSGEAALAFADAHPEVDVLAADVHTPGIAHLLLALDADPRPNVWVARADALD
                     LLDHDLPTGSLAGVHVFFPDPWPKARHRKRRFIRADVLDLLADRMAPGGHLLVATDVE
                     DYARHAQAELDAHPDFDGGPGERPAWRPVRGYEAKGASAGRSIAELAYRRR"
     gene            8035..9408
                     /locus_tag="VLR27_08515"
     CDS             8035..9408
                     /locus_tag="VLR27_08515"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HSP03527.1"
                     /translation="MGLRRRDNPIHTLAGVCLVVLFAFAFGLPAAAQTETSDALAEAQ
                     AERDAVRDQQDRIAERIDVLGATDAQIAAALDELDAALAAQEAVLKETLAAADAAAAA
                     QREAEAELRTAERDVAILEQAIVEMAVASYVHPPTADLVQSLQAQSLSDAVIQQIYLD
                     ARAQRDVDLLDLLEEAEERAEARAAEVAAAVVAANEAVEAADAELIRLDDERQQQARF
                     AADLQGRIDGALAEAAALADLDADLSAQIVAEQAALIARLPPPPPPEPEVVAAPAPTT
                     PSTAPPTTVAPPTTAAPTPGSPAPTPAPTTTRPPTTTTRPPVTSAPRPSTPTPPLATV
                     WGFTVHAGIADDVDAMLTAARADGITFGGSAYRSTDRQIELRIQNCGPTDYDIWYRPA
                     STCSPPTAVPGRSLHEQGRALDLTFEGRLITSRANQGFQWLAANAASYGLFNLPSEPW
                     HWSTTGG"
     gene            complement(9484..10275)
                     /locus_tag="VLR27_08520"
     CDS             complement(9484..10275)
                     /locus_tag="VLR27_08520"
                     /inference="COORDINATES: protein motif:HMM:NF006108.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HSP03528.1"
                     /translation="MTASVHVEAVGTTTVVTIDRPDVRNAVDGPTALALHDAFIAFEA
                     DDAQNVAILTGASGTFCAGADLKAVSTGDGNRVEAMTAGAPDPEALGPMGPTRLRLSK
                     PVIAAVEGHAVAGGLELALWCDLRVAARDAVFGVYCRRWGVPLIDGGTVRLPRLIGQS
                     RAMDMILSGRPVEGPEAEAWGLANRLTDPGGALDGALALAEQLSAFPQHCLRSDRASA
                     LDQWSLSEWGALAHETELGLATIRSGETLEGASRFAAGEGRHGQF"
     gene            complement(10277..11182)
                     /gene="rimK"
                     /locus_tag="VLR27_08525"
     CDS             complement(10277..11182)
                     /gene="rimK"
                     /locus_tag="VLR27_08525"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HSP03529.1"
                     /translation="MKIGLLSRNANLYSTNRLKEAAEERGHDVRVIDYLRCVMDITSH
                     KPAIRYGGQELSPLDAVIPRIGATHTFYGTAVVRQFEMMNVFPTNESQGISRSRDKLR
                     SLQLLSRNGIGLPVTGIAHSTKDIDGLIELVGGAPLIVKLIEGTQGVGVVLAETKKAA
                     ESVIAAFRQLDANILVQEFIKEAKGADIRAFVIGGRVVASMRRQGAPGEFRSNLHQGG
                     HAEKIKLTPEERSTAVRAAKTMGLNVCGVDLLRSNHGPVVMEVNSSPGLEGIEQTTGV
                     DVAGKVIEFIEKNARPGNTRTSARG"
     gene            complement(11196..11666)
                     /locus_tag="VLR27_08530"
     CDS             complement(11196..11666)
                     /locus_tag="VLR27_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease"
                     /protein_id="HSP03530.1"
                     /translation="MGDPPAEDPRTLIGWREWIHLPDLLDGGWIKAKVDTGARTSALH
                     AWDVEQLDRDGATWVRFSLHPRQHDDEHVVQAEAPLVEEREVRSSNGEVEVRPVVETS
                     LALGGQRYAIELTLTKRDQMGFRMLLGRTGMARHLLVDPGSSYLLGGDRHRPPG"
BASE COUNT         1583 a         4161 c         4379 g         1644 t
ORIGIN      
        1 ggtcgggggc acgtccctgg tggtgggctc gaggtcgtgg cccacccgcc aggcccggtg
       61 gccggcggcg tgctcggcca gcagccatgc gacgccgtcg tcgtcgagga ggggctcgcc
      121 gatcgcctcg tcgccatcga ggtgcagggt ggtcggctcc ttgcccgggc gctggatgct
      181 gacggcgccg tcacggtcca ccaccagcac gtcgtcgccc acgagcaccg ctccctgggt
      241 ggggacgtgg agggaccgcg cgtcgtggag cccgtcgtcc acgaccacca cgccgtcggg
      301 gccggcgagg agcgtggcgc tggagcccga ggccgcgaac cgggtgctgg gcggcagatc
      361 gcctccggcc cgggcccggg cctcgaccag gccggagaac ccgttcagga gcaccagccc
      421 gggtccgttc ccggtgggca ccgacatcca cgtgcgcccg tcgacgtcgg cggggcgcac
      481 ggcatccggg gggctggcca cggtgatgcc gaccaccgtg gcgaggaccg cgacggtcgt
      541 gacgagcacg atggtggcgc gatcgaggcg gagcagccgc cggacagtgg gggacatcgt
      601 gcttccgccg tgggagctga gggggacgcc tggactcttc gccggccggg cggggccgtc
      661 ctcctcagac gagaactgga tcggtcaccg gctgtcctcc ggtgaccgcg ctcagtgggc
      721 ggcgcgcagc acgacgatcc ccgtgacgag gccccacagc gggaccagga ggaacgggag
      781 gaacatcggg ctgaggagca ggagggcggc gacgacgaac cccccggtgc tgagccaccc
      841 gggcagcgtc cccgttcgcc gcgccgccca cgaggcgagg gcgacggcca gggccatgga
      901 ccagccggtc ccgacgagca tcgtgccgag gctcagctga tcgagcgagc ggatgagctc
      961 accgcccggc gccgcgaagt ccggtgcgaa ggtcacgccc gcggccatcg cgccaccgac
     1021 cagggcggcg atcaccaggg tgacgccggc catgatcccg gcgccccgca ggacggtgat
     1081 gggcggatcg tcctcgatcc cggcgccgcg caccagcgtc gcgagggtgg tgacgaaggc
     1141 catgagcagc aggccggcgg cggtcaggag catggtgccg atgaccgtcg tgacgttgtc
     1201 gttctccacc cagctgaccc agtcggcttc gggtgcgtcg tacgcgggga cctgggcgac
     1261 gaggccgaag ccggcgatca gagcggtggc gaagcccacg ccggcccatc cggcccagcg
     1321 ggcggacccg ttgccccgcg gcggggcggt gcggctggtg ggagcggtgg tggtggtcgt
     1381 tgtggttgcg gtcatgtcag gttccttcct caatcaacgg tgagctcgcc ggccatgccc
     1441 tcgacgaagt ggggcgggcc ggtgccctcg gtgtcgccga cggtgccgac ggggatgaag
     1501 cagacgtagg cccagcggcc gggggagagt tcggtgacgg tgccggacgc ggcgccggcc
     1561 ggggcgaacg tcccgccgcc gaaggcctcg atgtgctgct ccgcctcggc ctcgggcatc
     1621 tcgaggagct cctcgatcga cagctcggtg tcgtcgctga gcttgaccag gctgaactcg
     1681 tgggcctccc ccgcttcgct gtcgttggac atgacgaacg aggtggcgcc ggcgggcagc
     1741 gtcgacggca gcccctcgta gccgtagtcg atggcgctga actcgacgac ctccacgtcg
     1801 cagctgtcgt cgaggctccc gtagacggcc tcggaggcgg ccaggaactc gggggtctcg
     1861 aaggccgaga agtcgccggt cgaggtcacc tccgtcaccg ccgccgtcat ggtctgcacg
     1921 tctgcggcga cgtcggccgg ctcgttgccc tcgatgaggg cgaggttggg gtcgatgcgc
     1981 tccgcgacga aggccgggat ctcggcctcg tcctcgggtg cctcggcgaa ggccgcctcc
     2041 agacccagga agccctcgca gaacgagtcg tcggaggctc cctcgtcgct cgccggctcc
     2101 tcgtcggtcg cagcaccctc atcgggagcc gacaccgcct ccgcggtggg atcgtcgctg
     2161 ccctcggcgg tgtcgtcgtc atcgtccccg caggcggcga ggacggacgt ggccaggacg
     2221 agggcggcga gggccgccga tgctcgggcg atggggtggc ggtgtgcggt ggtcatgtcg
     2281 ggttcccttc ggttcggttc tcggtgcgta cggcgaggcc gtcgatctcg acctccatgc
     2341 cggggtcggc gaggccgcgc acctcgacgg tggtgatcgg cgtggtcacg gcgtgctcga
     2401 cgaggtgctc gcggaccacg aactgcgcgt cgagcagcgc agcgcggctg gtggcgtgga
     2461 tgcgcaggtg ggccaggtcg gccaaggtca tcccggcagc gacgacgacg gcggtcaggt
     2521 tgtcgagcgc cagcgccacc tgggcgatcg catcgccctc gtgcatgagc cggccctcgg
     2581 cgtcgacggg acgctgcccg ccgatcgaga ggatggcgct gaccccgtcg cggcgctggg
     2641 cctggtcgtg gccgagccgc ctcgaccagt ccgctgcgtt cacgctgcga gcgcggcgtg
     2701 ccgtcggtgt cgtgtcggtc atcagcccga tcatccgggc ggcactgcgg gcgagcgatc
     2761 ccacgtgctc ggggacctcg acaccccagg ttgttgggat ggcggaccga gcccgatcca
     2821 ggcatgatcg acgggtgggg aaggcgttgg cgatcgagcg ggttcgttcc gacgtcgagg
     2881 tgctggcccg ggccggtctc gacgtgtcga cgttcctcgc cgaggtcgat gcctccctcg
     2941 tgcgggccgt cccctacgag gccatgtgcg tcgcgctgca ggacccggcc acgtcgatgg
     3001 cgactgccac gttcaagttc ggcgggatct cggggcgcga gtcccacgac ctgcactggt
     3061 gcgagatcga gttcggcgcc gcggacgaca cgagcttccg ctcgctgacc gaccgcggcg
     3121 tcacggcggt gggcctccac cacgagaccg gtggcgacgt cgctcgctcg gtgcgcatgc
     3181 gcgacttcat gtgccaccac ttcggcttca ccgacgagct gcgcaccgtc gcgcgcgtcg
     3241 acgaccgcga ctgggcggcg ctggcgatga tgcgggcgga tgccgccaac cccttcgacg
     3301 acgccgagat cgcgctgatg tcgtcgctgt cgggcgcgct ggccaccggc atccgcgccg
     3361 gtgtgctggc ccgctgcgcg gactcgtcgg cgacgacgct cgagggcccc gcggtcatca
     3421 ttgtcggacg cgacgggtcc ttcacccagg tcaacccggc ggccacccac tggctcgccg
     3481 agctggcgag ctgcgacaac agcgagggcc cggtgcacac cctggccgcg ctgatcgacg
     3541 gcgcccgccg gttcgcccgg ggcgaggtcg acacgctgcc ccgctgccgc gtgcgggtgc
     3601 cctcgggtac ctggctcgtg ctcaacggtt caccgctgtc cgggcccgac agccccggca
     3661 ccgacatcgt catcaccatc gagcaggccc ggccaccgga gatcgtgccg ctcgtcgtcg
     3721 ccgcgttcgc gctcacgccg cgcgagcgcg acgtcaccca gctggtcctc cagggcgtcc
     3781 ccagcaagga ggtggccagc acgctgcaca tctcggtcca caccgtgcag gaccacctga
     3841 agtcgatctt cgagaaggcc gacgtccgga gccgccgcga gctcatcgcc cgggtgttct
     3901 tcgaccagta cctgccccgc ttcggtgacg aggtcacgac ctcggggtgg ttcgcctgac
     3961 gatcgctgaa gcgtcgagcg ccggcctcag ctggcgagcg tggccggtcg ggggtcgacg
     4021 gccggggcgg ggttggagaa ctccatgacc ccgtcggtga cctcgctgcg cttcagcgcc
     4081 cggtagtcgc gcaggtagct ctggtgcacc tgccacggca tgcgggaccc ctgcttgggg
     4141 aaccggtcgg cggcgcgctg gatgtagccc gacgacagcc cgagcagcgg cgccggcgtg
     4201 accgaggcgt ccgcgttcac cggcgtcgcc tggcgcagcc cggtggcccg catgtggttg
     4261 agcatccggg tgacgtagtc gcaggtgagg tcgcacttca gcgtccacga ggcgttcgtg
     4321 tacccgatgg ccagggccag gttcggcacg ccctcgagca tcatgccctt gtaggtgagc
     4381 ttgctggcga tgtcgaccgg ctcgccgtcg acgctcgcct cgatgccacc gatgaagagc
     4441 agctcgagcc cggtggcggt gacgatgatg tcggcagcca gctcgcgacc ggactgcagc
     4501 cggatgccgg tctcggtgaa ggtgtcgatg tggtcggtga ccaccgagac gctgccctcg
     4561 cggatggccc ggaagaggtc gccgtcgggg acgaggcaca cccgctggtc ccacgggtcg
     4621 tagctcgggg tgaagtgcgt gtcgatgtcg tagccctccg gcaggaggcc ctcgaggcgc
     4681 ttgcgcagga gcttcttcac catgccgggg aaccgctggc tcagctggaa gaacccctgg
     4741 gagcccaggg cgttcatcca cttcacgatc gggccggaca gtcgggtcgg caggagccgc
     4801 tgaaggaggc gggcgagggg gttcttcgcc ggcatcgaca ccacgtagga cggtgagcgc
     4861 tggagcatgg tgacgtgggc ggcgccagac ccgtcaccgg ggtcggccat ggacgggacg
     4921 agggtgacgg cggtggcgcc gctgccgatg acgacgacct gcttgccggc gtagtcgagg
     4981 tcctcgggcc agtgctgggg gtggatgacc tggccggcga agtcgtccat gccggcgaag
     5041 tcgggctggt agccgtggtc gtagcggtag tagccgctgc aggagaagat gaacccggcg
     5101 gtgagctcga tgcgctcggg ggcggcatcg gggcggtcgg ggtcggtccg ctcggcggtg
     5161 atgtgccacc gggcgtcctc ggtcgaccag tccgccgtca cgacccggtg gttgaagcgg
     5221 atctggcgat cgatcccctc gacgcgggcc gtctcccgga tgtactcgag gatggaggcg
     5281 ccgtcggcga tggacttctc gccggtccag ggccggaacg agtagccgag ggtgtacatg
     5341 tcggagtccg agcggatgcc ggggtaccgg aacaggtccc acgtgccgcc gatggcgtcg
     5401 cgagcctcga agatggcgta gctggcccac gggcactccg cctgcaggta gtggccggcg
     5461 ccgatgccgg acaggcctgc gccgacgacg aggacgtcga ggtgctcggt gctcacgtgg
     5521 gttcctccag tgggttactc cgggtaggtg actcaccgta acgcagggtg cccgtgctcg
     5581 tcacgtggtg atccgtcaca tctcggacaa tcagggcctg acgaaggtgg cccactcctc
     5641 gaggggcatg cgctcgctgc gcagggagtt gaccggtgcc tccgggtcgg cgtggccgac
     5701 ggcgacgccg cagaagagca tctcgtcgtc gggcgcgccc acgaactcgg cgaccgcctc
     5761 ggaccacacc gcccaggcct cctgggggca ggtgtcgatg cctcgctcgg tgcacagcag
     5821 catgaaggtc tgcaggaaca tgccgaggtc ggaccactgc ggcggcccca tgcgccggtc
     5881 gatgaagcag aacagcccga ccggtgcgtc gaagaaccgg tagttgcgcg ccaggtggcc
     5941 gaagcggccc gccttgtcct cccgggggat cccgagggtc tcgtacatct gctcgcccag
     6001 ctggaaccgg ttggtgcggt acggctccca cagccccgac gggtagatct cgtagccggg
     6061 cgtctccatc tggcgtcctt cgatgaagga caggaacttc ggcatcgtgt cggcaccgat
     6121 gacgtagatc cgccacggct gcacgttccc gcccgacgga gcccgggccg ccagctcgag
     6181 cagctcggcg acggtgtcgt cgtcgacggg cgtgtcgagg aaggcgcgga tcgacgtgcg
     6241 gcgccgcacg gcttcggtga cgtccatcac gccgatcctc ccagctcggc ggccagcgac
     6301 gcgcacgggt ggtcgggggc gggctgccag ggggacagac cggcggaggt cgccagcagc
     6361 ggcgccggcc acagcaggcg gctctcgccg gcgacgatgg acttggtgct ggccaggatc
     6421 gagctcagca ggtcgtgggc ggacggatcg accaccacga gctcggtggg catgggcgcg
     6481 agcaccacgg cctggtcgag cccgcaggcg gcgagcagcc ggtcgggcgc ggccagccac
     6541 gccggcgggt ggtcggccgg gctcgtcggc acccaggccg gctggcccgg gccgaccggg
     6601 tcgaggccgg ggtcggtcgc cgccaggttg gtcagcgcgg cggcgcggac gtcgtcggca
     6661 ctcacctccc acgatgcggc atcgccggca tcgaccggga gcgcctccgg tccgccgagc
     6721 acgacgcgtg cttcgagcac gccggcgatc ggccaggtga tggcggccgg gtgctcaccg
     6781 gcgcaaccga cgaccacgcg caggcggtcc cgcgccgcat cccagccggt gccgccggcg
     6841 ggtgccggcg ccggatcgtc tgacggggct tgtgtgccgg tgacctggac gtccggggcg
     6901 acgtcgggcg gggtgggatc gacgccgggc tcctcggcac cctcgggcgg gccggtcggc
     6961 ggggcgaccg caccgatcag ggcatccatg atctcgatgc gctcgaagcc gttggccttc
     7021 gtccagccct cccgggccgc cgccagcgac agcgtggacg ggtacgggtt gtcggcccag
     7081 gcgatctgca cggtgtcggc atccaccggc tcggcgccga cgacgcccgc cacgacccgc
     7141 aggcgggcca ccgcctccca ggtcatcgcg tccgggccgg gtgccttctt gaaacggtcg
     7201 aggagcccca tggcgggcga cggtaccccc cgctctcgaa ctggcagtgc tgatggagcg
     7261 ccgctcaccg ccggcggtac gccagctccg cgatcgatcg cccggccgac gcccccttgg
     7321 cctcgtaccc gcggaccggg cgccacgccg ggcgctcgcc cggcccgccg tcgaagtccg
     7381 ggtgggcgtc gagctcggcc tgcgcgtgcc gggcgtagtc ctcgacgtcc gtggccacga
     7441 gcaggtgacc gcccggagcc atccggtcgg cgagcaggtc gaggacgtcg gcccggatga
     7501 agcgccgctt gcggtgccga gccttcggcc acgggtcggg gaagaacacg tgcacgcccg
     7561 cgagcgaccc ggtcggcagg tcgtggtcga gcaggtcgag ggcatcggcc cgggcgaccc
     7621 agacgttggg gcgggggtcg gcgtccagcg cgagcagcag gtgggcgatg cccggggtgt
     7681 gcacgtcggc ggcgaggacg tcgacctccg ggtgggcgtc ggcgaaggcg agcgcggctt
     7741 caccgctccc gcaccccacc tcgaggacca ggggacgcgc cgggtcccgg tgggcgacgt
     7801 cgtggcgggg cccgatctcg gcgagggcgg cgcgcatgac gtcggtgatc cgcccgtggc
     7861 gggcgtggaa cgtgcggatg cgagggtggc cgtcgagcac gtccccatgg tcgcagcccg
     7921 ccggagcacg ctcctcaccg tgcggagcgc gagggcggtg ctacccagcg tgttcgttgt
     7981 tgcggtacgg tgacgacgcc gatcgggcgg cggttcgacg cttgaggtct gcccatgggg
     8041 ctgcgacgac gcgacaaccc gatccacacc ctcgccggtg tgtgcctggt cgtcctcttc
     8101 gcgttcgcct tcgggctccc cgcggccgcc cagaccgaga cctccgacgc cctcgccgag
     8161 gcccaggccg agcgcgacgc cgtacgggac cagcaggacc gcatcgccga gcgcatcgac
     8221 gtgctcggcg ccaccgacgc gcagatcgcc gccgccctgg acgagctcga tgccgcgctc
     8281 gccgcccagg aggccgtgct caaggagacc cttgcggcgg ccgacgccgc ggcagccgcg
     8341 cagcgggagg ccgaggccga gctgcgcacc gccgagcgcg acgtcgccat cctcgagcag
     8401 gccatcgtcg agatggcggt ggcgtcctac gtgcacccgc ctaccgccga cctggtgcag
     8461 agccttcaag cccagtcgct gtccgacgcc gtcatccagc agatctacct cgacgcccga
     8521 gcccagcgcg acgtcgatct cctcgacctg ctcgaggagg ccgaggagcg ggccgaagcc
     8581 cgggccgccg aggtcgctgc cgcagtggtc gcggccaacg aggcggtcga agccgccgac
     8641 gccgagctga tccggctcga cgacgagcgc cagcagcagg cccgcttcgc cgctgacctg
     8701 cagggccgca tcgacggcgc gctcgccgag gccgcggccc tcgccgacct cgacgccgac
     8761 ctctccgccc agatcgtggc tgagcaggcc gccctcatcg cccgcctccc cccgccaccg
     8821 ccgccggaac ccgaggtcgt cgctgcgccg gcaccgacca cgccgtcgac ggctccgccc
     8881 accacggtgg cgcccccgac cacggcggcg cccacacccg gctcccccgc ccccaccccg
     8941 gccccgacca cgacgcgtcc cccgaccacg acgacgcggc cgcccgtcac cagcgcgccc
     9001 cgcccgtcca cgccgacgcc tccgttggcc accgtctggg gcttcaccgt gcacgccggc
     9061 atcgccgacg acgtcgacgc catgctcacg gccgcgcgcg ccgacggcat caccttcggt
     9121 ggcagcgcct accggagcac cgaccgccag atcgagctgc ggatccagaa ctgcggcccc
     9181 accgactacg acatctggta ccggccggcc agcacgtgct caccgccaac ggcggtgccg
     9241 ggccgctccc tgcacgagca gggccgggcg ctggacctca ccttcgaggg tcgtctcatc
     9301 accagccggg ccaaccaggg cttccagtgg ctggccgcca acgccgcgtc ctacgggctg
     9361 ttcaacctgc cgagcgagcc ctggcactgg agcaccaccg gcggctgacg gcccccatcc
     9421 gttctggcct acatccatga ccaccaggtg gcacatcggt aggccagaac gatccctggt
     9481 cgttcagaac tggccgtggc ggccctcgcc ggcggcgaag cggctcgccc cctccagcgt
     9541 ctccccggat cggatcgtgg ccaggccgag ctcggtctcg tgggcgaggg caccccactc
     9601 ggacagcgac cactggtcga gggcggaggc ccggtccgag cgcaggcagt gctgcgggaa
     9661 cgccgacagc tgctcggcca gggccagcgc gccgtcgagc gcgccgcccg ggtcggtgag
     9721 gcggttggcc aggccccacg cctcggcttc gggaccttcg accggccggc cggacaggat
     9781 catgtccatg gcccggctct ggccgatgag gcgggggagg cggaccgtgc cgccgtcgat
     9841 gagcgggaca ccccaccgcc ggcagtagac gccgaagacc gcgtcgcggg cggcgacccg
     9901 caggtcgcac cacagcgcca gctccaggcc gccggccacc gcgtggccct cgacggcggc
     9961 gatcacgggc ttggacagtc gcaggcgggt cggccccatc ggtccgaggg cttcggggtc
    10021 gggcgcgccg gcggtcatgg cctcgacccg gttgccgtcg ccggtcgaca ccgccttgag
    10081 gtcggcaccg gcgcagaacg tgccgctcgc cccggtgagg atcgccacgt tctgggcgtc
    10141 gtcggcctcg aaggcgatga acgcgtcgtg cagcgccagg gcggtcggcc cgtcgacggc
    10201 gttgcgcacg tcgggccggt cgatggtgac gaccgtggtg gtcccgaccg cctcgacgtg
    10261 gacgctcgct gtcacgtcag ccccgggccg aggtccgggt gttgccgggc cgggcgttct
    10321 tctcgatgaa ctcgatgacc ttgccggcca cgtcgacgcc ggtggtctgc tcgatgccct
    10381 cgagcccggg ggaggagttg acctccatca ccaccggccc gtggttggac cggagcaggt
    10441 cgacgccgca gacgttgagg cccatggtct tggccgcccg gacggcggtg gagcgctcct
    10501 ccggcgtgag cttgatcttc tcggcgtggc caccctggtg caggttcgac cggaactcac
    10561 ccggcgcgcc ctggcggcgc atggacgcca cgacccgccc gccgatcacg aaggcgcgga
    10621 tgtcggcgcc cttggcctcc ttgatgaact cctgcacgag gatgttcgcg tcgagctggc
    10681 ggaaggcggc gatgaccgac tcggccgcct tcttggtctc ggccagcacc acgccgacgc
    10741 cctgcgtgcc ctcgatcagc ttcacgatga gcggggcgcc gccgaccagc tcgatgaggc
    10801 cgtcgatgtc cttggtcgag tgggcgatgc cggtgacggg cagcccgatg ccgttgcggg
    10861 acagcagctg gagcgagcgg agcttgtccc gggaccggga gatgccctgg ctctcgttgg
    10921 tcgggaacac gttcatcatc tcgaactggc gcacgaccgc ggtgccgtag aaggtgtgcg
    10981 tcgcgccgat gcgggggatg accgcgtcaa gggggctcag ctcctgtccg ccgtagcgga
    11041 tggccggttt gtgcgaggtg atgtccatca cgcagcgcag gtagtcgatg acccgcacat
    11101 cgtgaccgcg ctcctcggcc gcctccttga ggcggttcgt cgagtagagg ttggcgttgc
    11161 gggacaggag tccgatcttc atacggtcgc cgacgctacc cgggcggccg gtggcggtcg
    11221 ccgcccagca ggtacgagct gcccgggtcc accagcaggt gccgggccat gccggtgcga
    11281 ccgagcagca tgcggaagcc catctggtcc cgcttggtga gggtgagctc gatcgcgtag
    11341 cgctgcccac cgagtgccag ggacgtctcc accaccggcc ggacctccac ctcgccgttc
    11401 gacgagcgga cctcgcgctc ctcgacgaga ggcgcctcgg cctgcaccac gtgctcgtcg
    11461 tcgtgctggc gggggtggag cgagaagcgg acccaggtcg cgccgtcccg gtcgagctgc
    11521 tcgacgtccc aagcgtgcag cgcggaggtg cgtgcgccgg tgtcgacctt ggccttgatc
    11581 cacccgccgt ccaggaggtc gggcaggtgg atccactccc gccagccgat cagcgtgcgc
    11641 ggatcctccg ccggcgggtc ccccacgatc aggtggtcgg ggtgcccgct gcggcggtga
    11701 ccggctcggc cacctcggcg agggcggcgc ggtcggctgc cgagtggaac accggcgtcg
    11761 gaggcgc
//
LOCUS       DATISU010000206         6678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_569069, whole genome shotgun sequence.
ACCESSION   DATISU010000206 DATISU010000000
VERSION     DATISU010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569069"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /gene="ppk1"
                     /locus_tag="VLR27_08535"
     CDS             <1..1666
                     /gene="ppk1"
                     /locus_tag="VLR27_08535"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HSP03531.1"
                     /translation="HVFPVLTPLAVDPGHPFPYVSNLSLNLAVLVADPEGGERRFARV
                     KVPSNIDRFLRVPGTGLHVALEELIADRLDELFPSMAVIEWSMFRVTRNADLTVEDNE
                     ADDLLEAVEMELRRRRFGRAVRVEVGADMSPSLQDLLVRELDLTPDDVYVVDGSLDLT
                     ALWSVHAVDRPDLKDRHWHGTTHWHSAGGGDVPADFCAEIRRGDILVHHPYSSFRNTV
                     EAFLRQAAKDPGVVAIKMTLYRTSGESSIIRSLVRAAESGKQVAVLVELKARFDEARN
                     IEWAKALEEVGVHVVYGLVGLKTHTKTLLVVREERDGLHRYCHIGTGNYNPKTAMIYE
                     DIGLFTTDPAIGTDLSQLFNYLTGFGRAVDYEKLIVAPDHLRSRLEELVANEATYGSS
                     GKIVAKMNALSDAGMIDTLYQASQADVSTDLVVRGICCLRPGVPGLSDNIRVRSIVGR
                     YLEHSRIYYFGNGNGRGSPAWFIGSPDLMGRNINRRVEALVPVETPSNQLRLQEILDV
                     VLADDRLAWELDGDGRWNRVADTGAGIDSHVRLQELALGRTRRREG"
     gene            1671..2084
                     /locus_tag="VLR27_08540"
     CDS             1671..2084
                     /locus_tag="VLR27_08540"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSP03532.1"
                     /translation="MADEPEVRAAGGIPWRHAGDALEVLVIRRPRYDDWTFPKGKLDP
                     GETWEQAAVREVWEETGIVAVLSTEIAGTEYRDRYGCAKQVRYWSMPVAADAGFEPND
                     EVSERRWMSVAEAARLLSYDHDRRVLDSLVGLLER"
     gene            2226..3203
                     /locus_tag="VLR27_08545"
     CDS             2226..3203
                     /locus_tag="VLR27_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSP03533.1"
                     /translation="MSRNRRRFPSGALLLPVVLVAAACVRDTETVVVSGSSTVEPISI
                     AVAEGFADERDEVSISVDGPGTGDGFELFCDGLIDINDASSKIKPEQIEQCEAGGVEF
                     VELQIANDGIAVLTNPDNDTVECLTLADLYALVGPESRPVARWSDAEPLARELGSSTD
                     LPDAAIDIVGPGEESGTFASFVELVIEEFNEDRGQEATTRPDYQASSDDNIILQGVGG
                     SATSFGWVGFAFASGAEGVKILGIDGGEGCVAPTAETIADGSYPISRPLFIYVNADAA
                     ATSPVLEAFVDFYLHDAIDAVADVGYVPLSDEDLDATRERWASRTTGAA"
     gene            3200..4138
                     /gene="pstC"
                     /locus_tag="VLR27_08550"
     CDS             3200..4138
                     /gene="pstC"
                     /locus_tag="VLR27_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HSP03534.1"
                     /translation="MSDLTTPAAYTLEDFRGSKKRMRTEHLVRAVLFVAALLSIVISV
                     LIVASLVGEAWTFVSQVEAASLWSDGWFPRRGIYDIKTLLAGTLIVTVIAMAVAGPLG
                     LGAAIYLSEYAPRGVRKLLKPVLEILAGIPSVVLGFFALTFLSPVIVQRLSDEATQFN
                     IAAAGIGVGILTIPLVASISEDAMRSVPMALREASYGLGARKITTTIRVVIPAAVSGL
                     VAAFIVAVSRAIGETMVVFIAAGATGGSLFSTDPFKPGQTMTAAIASQAAGTDQVRGA
                     GLTFQSLFFVALLLFVITLVLNVLAGRFVQRVRQAY"
     gene            4151..5110
                     /gene="pstA"
                     /locus_tag="VLR27_08555"
     CDS             4151..5110
                     /gene="pstA"
                     /locus_tag="VLR27_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HSP03535.1"
                     /translation="MALTTPAAQIGAIVPEQVRRQLTGRRSDTAGRIFQAVLIGAVAA
                     SVAVLGVLVVDMVQTGSGTLTERLGSFLTGSLRSQPGESGLLQGLRGTFYIGVFTIVF
                     AFPIGVGAAIYLEEYAAKSRLSRFIDLNIRNLAGVPSVVYGILGLAIFVKALGSITGP
                     SSQGRSLISAGLTLAILVLPIVIITASEALRAVPGSLREAGYGVGATRWEVTRDHVLP
                     YAAPGILTGTVLALARAMGEAAPLILVGAVTGFLSGGTELDPSTLGDRFTAMPIIITT
                     WAGRPGPEWEQATAAAIVVLLVVVLLANAVAILLRNHYEKKRG"
     gene            5112..5978
                     /gene="pstB"
                     /locus_tag="VLR27_08560"
     CDS             5112..5978
                     /gene="pstB"
                     /locus_tag="VLR27_08560"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HSP03536.1"
                     /translation="MPEHSTEERPTMTDAPMVGSMPTSAPSQGGATVFDVDDVSVYYG
                     DFRAVRDVSLQVKQHQITAFIGPSGCGKSTMLRCFNRMNDLIDTARVEGGISYHGVDL
                     YDPAVNPVEVRRRIGMVFQKPNPFPKSIYDNVAYGPKVAGAKGNLDDVVEQALQGAAL
                     WDEVKDRLKESGMSLSGGQQQRLCIARAIATDPEVLLMDEPCSALDPIATGRIEDLMV
                     EIKERFTIIIVTHNMQQAARVSDRTAFFTTEVNEESDRRTGLLVEYADTDKIFTTPDD
                     ERTENYVTGRFG"
     gene            6032..>6678
                     /gene="phoU"
                     /locus_tag="VLR27_08565"
     CDS             6032..>6678
                     /gene="phoU"
                     /locus_tag="VLR27_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HSP03537.1"
                     /translation="MRKSFHDELDQLRLQVELMGVKVDHNLERTRRVLLDGDPDAAAA
                     AVACDDEIDAMNVSLTERCYDLLARENPVASDLRFVVSVLRILSELERIGDLALRVVK
                     LNPERELWAGHRPTFDILVSMGDTAIDAYRDALRAWSAQDLDLATDLATRVRAMDMHY
                     EHLMAELLRLNGPYAVPIATATLIGGRSLERIADHSAIIGARLRYMLTGDPDHLTA"
BASE COUNT          987 a         2442 c         2229 g         1020 t
ORIGIN      
        1 ccacgtcttc ccggtgctca ccccgctggc cgtcgatccc gggcacccgt tcccctacgt
       61 ctcgaacctg tcgctcaacc tggccgtcct cgtcgccgac cccgagggtg gggagcgacg
      121 cttcgcccgg gtgaaggtgc cttcgaacat cgaccgcttc ctccgggtgc ccggcaccgg
      181 gttgcacgtc gcgctcgagg agctcatcgc cgatcgcctc gacgagctgt tcccgagcat
      241 ggccgtcatc gagtggtcga tgttccgggt cacccgcaac gccgacctga ccgtcgagga
      301 caacgaggcc gacgacctgc tcgaagcggt ggagatggag ctccggcgtc gacggttcgg
      361 tcgtgccgtg cgcgtcgagg tcggcgccga catgagcccg tcgctgcagg acctcctcgt
      421 gcgggagctg gacctgaccc cggacgacgt ctacgtcgtg gacggatcgc tggacctgac
      481 ggcgctctgg tcggtccacg ccgtcgaccg gcccgacctg aaggaccgtc actggcacgg
      541 caccacccac tggcactccg ccggtggggg cgacgtgccg gccgacttct gcgccgagat
      601 ccgacgcggc gacatcctcg tccaccaccc gtactccagc ttccgcaaca ccgtggaggc
      661 cttcctccga caggcggcca aggaccccgg cgtcgtcgcc atcaagatga ccctctaccg
      721 cacgtcgggc gagagctcga tcatccggtc gctggtccgg gcggccgagt ccggcaagca
      781 ggtcgccgtg ctcgtggagc tgaaggcccg cttcgacgag gcccgcaaca tcgagtgggc
      841 caaggccctg gaggaggtcg gcgtccacgt cgtctacggc ctcgtcgggc tcaagaccca
      901 caccaagacc ctgctggtgg tgcgcgagga gcgcgacggc ctccaccgct actgccacat
      961 cggcacgggc aactacaacc cgaagacggc gatgatctac gaggacatcg gcctcttcac
     1021 caccgacccc gccatcggca ccgatctgtc ccagctgttc aactacctca ccggcttcgg
     1081 gcgcgccgtc gactacgaga agctcatcgt ggcgccggac cacctccggt cccgcctcga
     1141 ggagctcgtc gccaacgagg cgacctacgg gtccagcggc aagatcgtgg ccaagatgaa
     1201 cgccctgtcc gacgccggga tgatcgacac cctctaccag gcgtcccagg ccgacgtgag
     1261 caccgacctc gtcgtgcgcg gcatctgctg cctgcgcccc ggcgtgcccg ggctctccga
     1321 caacatccgg gtgcgctcca tcgtgggccg ctacctcgag cacagccgca tctactactt
     1381 cggcaacggc aacggccggg ggtcgccggc gtggttcatc ggctcgcccg acctcatggg
     1441 gcggaacatc aaccggcggg tcgaggccct ggtgccggtg gagacgccct ccaaccagct
     1501 gcgcctgcag gagatcctcg acgtcgtcct ggccgacgac cgcctggcct gggagctcga
     1561 cggcgacggc cggtggaacc gcgtcgccga cacgggtgcc ggcatcgact cccacgtccg
     1621 cctgcaggag ctcgcgctgg gccggacccg gcgacgcgag ggctgaggtc gtggccgacg
     1681 agcccgaggt gcgtgccgcg ggcgggatcc cgtggcgcca cgcaggagac gccctcgagg
     1741 tcctcgtcat ccgtcggccg cgctacgacg actggacgtt cccgaagggc aagctcgacc
     1801 cgggggagac ctgggagcag gccgcggtgc gggaggtctg ggaggagacc ggcatcgtgg
     1861 cggtgctcag cacggagatc gccggcaccg agtaccgaga ccggtacggg tgcgccaagc
     1921 aggtgcgcta ctggtcgatg cccgtggctg ccgatgccgg cttcgagccc aacgacgagg
     1981 tgagcgagcg ccggtggatg tcggtcgccg aggccgcccg gctgctcagc tacgaccacg
     2041 accggcgcgt gctcgacagc ctggtcgggc tgctggagcg ctgaccgccc ggcctcgcca
     2101 gttcgcgccc aggtggccgt tcgttcacct tgcgttcacc tggcgcgggg ttcccgtcca
     2161 ccttgcgtcc gtagcgtgcg cagcgaccca cacgacgacc cggcccccac ctggaggaag
     2221 ccgtcttgtc acgcaaccgg cgccggttcc cgtccggcgc actgctgctc cccgtcgtgc
     2281 tcgtggccgc cgcgtgcgtg cgcgacaccg agacggtcgt cgtcagcgga tcgtcgacgg
     2341 tggagccgat ctccatcgcc gtggccgagg gcttcgccga cgagcgcgac gaggtgtcca
     2401 tctccgtgga cggcccgggc acgggtgacg gcttcgagct gttctgcgac ggcctgatcg
     2461 acatcaacga cgcctcctcc aagatcaagc ccgagcagat cgagcagtgc gaggccggcg
     2521 gcgtcgagtt cgtcgagctg cagatcgcca acgacggcat cgccgtgctg accaacccgg
     2581 acaacgacac cgtcgagtgc ctgacgctcg ccgacctgta cgcgctcgtc ggacccgagt
     2641 cccgcccggt ggcccggtgg agcgacgccg agcccctggc ccgcgagctc gggtcgagca
     2701 ccgacctgcc cgacgcagcg atcgacatcg tcggcccggg tgaggagtcc ggcaccttcg
     2761 cctccttcgt cgagctcgtg atcgaggagt tcaacgagga ccggggccag gaggccacca
     2821 cccgccccga ctaccaggcg tcgtccgacg acaacatcat cctgcagggg gtcggcggct
     2881 cggccaccag cttcggctgg gtcgggttcg cgttcgcctc cggcgccgag ggtgtgaaga
     2941 tcctcgggat cgacggtggc gagggctgcg tggccccgac ggcggagacc atcgccgacg
     3001 gctcctaccc catcagccgg ccgctgttca tctacgtcaa cgccgacgcc gccgcgacgt
     3061 ccccggtgct ggaggcgttc gtcgacttct acctgcacga cgccatcgac gcggtcgccg
     3121 acgtcggcta cgtccccctg tccgacgagg acctggacgc cacccgcgag cgctgggcct
     3181 cccgaacgac cggtgccgca tgagcgacct caccacgccc gccgcctaca ccctcgagga
     3241 cttccgcggc tccaagaagc gcatgcgcac cgagcacctc gtgcgcgcgg tgctgttcgt
     3301 ggccgcgctg ctctcgatcg tgatctccgt gctgatcgtc gcctcgctgg tcggcgaggc
     3361 gtggaccttc gtcagccagg tcgaggcggc cagcctgtgg agcgacgggt ggttccctcg
     3421 gcggggcatc tacgacatca agaccctcct ggccggcacc ctgatcgtca cggtcatcgc
     3481 catggcggtg gccggtccgc tcggcctcgg cgccgccatc tacctctcgg agtacgcgcc
     3541 ccgcggggtc cgcaagctgc tcaagccggt gctcgagatc ctggccggca tcccctcggt
     3601 ggtgctcggc ttcttcgccc tcaccttcct gtcgccggtg atcgtccagc ggctgagcga
     3661 cgaggccacc cagttcaaca tcgcggcggc cggcatcggt gtcggcatcc tcacgatccc
     3721 gctcgtggcg tccatctccg aggacgcgat gcgctcggtc ccgatggccc tgcgggaggc
     3781 gtcctacggg ctcggtgccc gcaagatcac caccaccatc cgggtcgtga tccccgctgc
     3841 cgtgtccggc ctggtcgccg cgttcatcgt ggcggtgtcc cgggccatcg gtgagaccat
     3901 ggtcgtgttc atcgccgccg gcgccaccgg cggctcgctg ttcagcaccg acccgttcaa
     3961 gcccggtcag accatgacgg cggcgatcgc ctcgcaggcc gccggcaccg accaggtccg
     4021 cggcgccggg ctcacgttcc agagcctgtt cttcgtcgcc ctgctcctgt tcgtgatcac
     4081 cctcgtcctc aacgtgctcg ccggacgctt cgtccagcgc gtgcggcagg cctactagga
     4141 gcgtccggac atggccctca ccacgcccgc cgcccagatc ggcgccatcg tccccgagca
     4201 ggtccggcgc cagctgaccg gccggcgctc cgacaccgcc ggccgcatct tccaggccgt
     4261 gctcatcggc gcggtcgccg cctcggtcgc ggtgctcggc gtcctcgtcg tcgacatggt
     4321 ccagaccggc agcgggacgc tcaccgagcg cctggggagc ttcctcaccg gcagcctgcg
     4381 gtcgcagccg ggggagtcgg gtctcctcca gggcctgcgg ggcacgttct acatcggcgt
     4441 gttcaccatc gtcttcgcct tcccgatcgg cgtcggggcg gcgatctacc tcgaggagta
     4501 cgcggccaag tcccggctgt cccggttcat cgacctgaac atccgcaacc tggccggcgt
     4561 gccgtcggtg gtctacggca tcctcggcct ggcgatcttc gtcaaggccc tcggcagcat
     4621 caccggcccg agctcgcagg ggcgctcgct gatctccgcc gggctgacgc tcgccatcct
     4681 ggtgctgccc atcgtgatca tcaccgcctc cgaagccctg cgagcggttc ccggcagcct
     4741 gcgcgaggcc ggctacggcg tcggcgccac ccggtgggag gtgacgcggg accacgtcct
     4801 gccctacgcc gcccccggca tcctcaccgg caccgtgctc gccctggccc gcgccatggg
     4861 cgaggccgcg ccgctgatcc tcgtcggtgc cgtcaccggc ttcctgtccg gtggcaccga
     4921 gctcgacccc tcgacgctcg gcgaccgctt caccgccatg ccgatcatca tcaccacctg
     4981 ggccggccgg cccggccccg agtgggagca ggcgacggcc gccgccatcg tcgtgctgct
     5041 ggtggtcgtc ctgctcgcca acgccgtcgc gatcctgctg cgcaaccact acgagaagaa
     5101 gaggggttga cgtgcccgag cactcgaccg aggagcgtcc cacgatgacc gatgcaccga
     5161 tggtcggctc gatgccgacc agcgcaccca gccagggcgg agccaccgtc ttcgacgtcg
     5221 acgacgtgtc cgtgtactac ggcgacttcc gtgccgtgcg ggacgtgtcg ctgcaggtca
     5281 agcagcacca gatcacggcg ttcatcgggc cgtcggggtg tggcaagtcg acgatgctgc
     5341 ggtgcttcaa ccggatgaac gacctgatcg acaccgctcg ggtcgagggt ggcatcagct
     5401 accacggggt ggatctgtac gacccggcgg tgaacccggt ggaggtgcgg cgtcggatcg
     5461 ggatggtgtt ccagaagccg aacccgttcc cgaagtcgat ctacgacaac gtcgcctacg
     5521 gcccgaaggt cgcgggtgcg aagggcaacc tggacgacgt cgtcgagcag gcgctgcagg
     5581 gtgctgcgct gtgggatgag gtgaaggacc ggctgaagga gtcggggatg agcctgtcgg
     5641 gtgggcagca gcagcggctg tgcatcgcgc gggcaatcgc caccgatccg gaggtgctgt
     5701 tgatggacga gccgtgctcg gcgctggatc cgatcgcgac gggtcggatc gaggacctga
     5761 tggtggagat caaggagcgg ttcacgatca tcatcgtgac ccacaacatg cagcaggccg
     5821 ctcgggtgtc ggaccgcacc gcgttcttca ccaccgaggt gaacgaggag agcgaccggc
     5881 gcaccggact gctcgtggag tacgcagaca ccgacaagat cttcaccaca ccggacgacg
     5941 agcgcaccga gaactacgtg acgggtcgct tcggctgatg cacccggcgc accagccact
     6001 gggcacgggg gacgggcaag ggggtcaacg gttgcgaaag tccttccacg acgagctgga
     6061 ccagctgcgc ctgcaggtcg agctcatggg cgtcaaggtc gatcacaacc tggagcggac
     6121 gcgccgcgtg ctcctcgacg gcgaccccga tgccgctgcg gcagccgttg catgcgacga
     6181 cgagatcgac gcgatgaacg tgagcctcac cgagcgctgc tacgacctgc tcgcccgcga
     6241 gaacccggtc gcctccgacc tgcgcttcgt ggtctccgtg ctccggatcc tgtccgagct
     6301 cgagcggatc ggcgacctcg ccctccgggt cgtcaagctg aaccccgagc gggagctgtg
     6361 ggccgggcac cgcccgacgt tcgacatcct cgtctcgatg ggcgacacgg ccatcgacgc
     6421 gtaccgagac gcgctgcggg cctggtcggc ccaggacctg gacctcgcca ccgacctcgc
     6481 cacccgggtg cgggcgatgg acatgcacta cgagcacctg atggccgagc tgctccggct
     6541 caacggaccg tacgcagtgc cgatcgccac ggcgacgctc atcggcggtc ggtcgctgga
     6601 gcgcatcgcc gaccactcgg cgatcatcgg agcccgcctg cgctacatgc tcaccggcga
     6661 ccccgaccac ctcacggc
//
LOCUS       DATISU010000207        15861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_475932, whole genome shotgun sequence.
ACCESSION   DATISU010000207 DATISU010000000
VERSION     DATISU010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 15861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15861
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475932"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2256
                     /locus_tag="VLR27_08570"
     CDS             <1..2256
                     /locus_tag="VLR27_08570"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP03538.1"
                     /translation="DGTEHVDLTASTRSPALTVLDDPRVRTATKALAALVAFYFVQQR
                     LWPAPAGVLLQGLVLGGLTALISFGIALIYRSNRIINFSAGELGAVPASLSVLLIVGP
                     GLPYILAVPLGLVAALALGAVVELVIIRRFFDAPRLILTVVTIGVSSLLAGVGFILPR
                     AFDITTPPQSFPSPFDFSFSVGATRFSGNDVVAMVAVPLCIAALGAFYRYTSIGIAVR
                     ASAESSDRAALLGVPVKRIQTIVWVIATALAFIAVFLRAGIVGLPFGQLLGPSILVKA
                     LAATVIGRMEKMPLILAASFALGILESAIIFDTGEAVLVDPIMFVIITAALLLQRSRK
                     SARGDETSNWQAATDFRPIPRELWALREVRWGVRSALAALVAATLALPLVMDEADISL
                     ATVIVVTATVAVSLVVLTGWAGQVSLGQIGFLGVGAAVGGWLTTTWTWDLSLAILAAG
                     VVGSLTAMAIGLPALRIKGLLLSVSTLSFALAASSYLLNRSFFDWLPVDRFDRPLLFG
                     RIALDTETRYFYFALAGLVIAIYLVKGLRRSRTGRVLIAVRENERAAQSYGVSATRAK
                     LLGFALSGFIAAYAGAIFVHHQQVFGIQPYAVGESLTVFIMAVFGGLGSIPGAIAGVV
                     LLEGIQYFRDAFPDYIADSLQFLASGVGLIVVLAVLPGGLSQAIYGMRDRWLRKVAAR
                     HDLHVPSLVADSRVEPAQIDAGPDEPDPADTATIVMVEQAGEVEAPVPVGAGAPTRPR
                     RSRPLRAGEDA"
     gene            2253..5288
                     /locus_tag="VLR27_08575"
     CDS             2253..5288
                     /locus_tag="VLR27_08575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP03539.1"
                     /translation="MTDTTRADALEAPSSTQRRLLAVTGGAATFPLVLLFGLNMVDEL
                     DRAAFGILVPEIRDAFGMSNSGILGVISLVSILALLAQPVFGIFADRTSRVRLAMGGA
                     SAWAIFSFLTGLVPTVALLVLARVGSGIGRAVNDPTHNSLLADYYEPDSRIRVYGFHR
                     AANYVGQTLGPLVGGGLAFAFSWRTPFLVFWVPTAILVLIGLRHLQDPIRGYHERKAG
                     GADEEASVTEEDQPSFGEAWRLCMQVRTLRRIWYSLPALAVSLVGFGSLSAIFYEEVF
                     ELNELQRGIIEAGLNPFQIAALLIGVPLATRYAGTNPALLMRLLAFVGAAVAGALVLF
                     ALSPNVATAVIAHIVVVAPLALLLPGIYAILSLVIPPRARGLGFGIGSLYVIPGLLLL
                     PIVGAVADDVGARQAMIVMVPVFLLGSGIISSAGAFVNEDVAKVRAATAAQSAVRLAR
                     ERGETKLLLVKDLDVAYGNVQVLFGVDFEVDQGEIVALLGTNGAGKSTLLKAISGLVE
                     PAGGATIFDGRDMTHATPQEIAARGVIQVPGGKGVFPGLTVAENLKIAGWLYQKDTEY
                     LEAATERVLEFFPILRERWDDKAGNMSGGEQQMLTLGQAFIAKPRLLMIDELSLGLAP
                     VIVEQLLGIVRAIRDQGTTVIVVEQSVNVALTLAETAYFMEKGEIRFHGPTKELLERP
                     DVLRSVFLEGAGAVSGSDTGDADQDNPFADLDLRTPALAARPRHVEPEASGPILSVAG
                     VGISFGGVRAVHDVSFDLGRNEILGIIGPNGAGKTTLFDLISGYLVTDTGRIELDGFD
                     ITHTSADARARLGMGRSFQDARMFPSLTVTENIAVALERHLEMRDPIAAALNLPAVAD
                     AERRVGERVDELVDLMNLGAFADKFVSELSTGSRRIVDLACVLAHRPDVLLFDEPSSG
                     IAQRETEALGPLLLRIREATGASLIVIEHDMPLISSISDRMLALELGQKVVEGPPRRV
                     IEDPRVVASYLGTTDEVINRSDAATERTDSTVDQGETQT"
     gene            5285..7126
                     /locus_tag="VLR27_08580"
     CDS             5285..7126
                     /locus_tag="VLR27_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03540.1"
                     /translation="MNDKTIADIRAFLTRFGPYLCLVGAIAAVMVFLPGDDDRTNVGT
                     TSAFGDFEDGDSTTVAAGRDGTTRTTTGATGATATGAGTGSTGASSGGGGGGGGGSGD
                     TGDGGGGGGGEAGGGEQAAPGEMVANCDLGTGRIAVPSHYAPPCVPAFGGDNGGETYQ
                     GVTGDKIKLAVMLVEGDAASEAVLSAAGANDSEEAVRQQYLDYVDYYSTHYETYGREI
                     EVTFYNGSGTATDDAAGRADAVRIANDGNFAVLNAPNNAFVDELVARGVMCMCTVSLP
                     GSFYEQRAPFVWTTLMDSFQGYIHRAEFIGKRLAGGKAEFAGDPTLALADRVFGLAYY
                     DDVQQSYRPGIDFFTEHLRDSWGISLAAVVGYRGYPDVAESQEAARPLIQKMIANDVT
                     SVICACDPFAPIFFTQEATRQNFRPEWIITGSALTDTAFFARTYDQTQWANAFGISFL
                     TVGLPDELGDDYRKLQWHHGRPPAAEATYGVIDGPTQIFFDGVHLAGPQLDPGTFRDG
                     MFRAGSRATGLTTAASSSFGTEVWGRPDYAALDDITMIWWDPQAQGEDETGQYGVGLY
                     QYMDGGRRYLPGQQPEGPLAAFDRAGTVSAYDGYPEGEAPPDYPPPG"
     gene            complement(7133..8329)
                     /locus_tag="VLR27_08585"
     CDS             complement(7133..8329)
                     /locus_tag="VLR27_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03541.1"
                     /translation="MLGIAMGWAVRHRRAAQAGVLALLVAGGALLALRAGDDAAAGPR
                     SGSELVDADPTQLLDPEPAPESYRIDYRVEGHGGDELIVTSDRLWVRRPWDSRLEVYP
                     GATPDGEPDAVQVSAFGGFRADGEDGDAVVVASPPGSATSDVRIAGALVRAVEDGRAE
                     LVERRRVAERTCQVVRSATTLATGDLSPATGPDEYADTCIDASGLVLEEVLVSEGEVL
                     LRRIATEVEISPDLDGVSFETGDPTLSVDEGGGFVAETEPGSRVPGRFFEPSTPPGFS
                     RHGRFAVVPAQAENFTEIDRLPQRLTYISDVFVDGPDVIVLDQGGTVGNVDPFPALQG
                     EDVDVAAGAGVLTYGLGGPTVIVDLGEGRFLRARGTVAPDLLIELLDGLEEVEGGELR
                     LLEPAG"
     gene            8391..9011
                     /locus_tag="VLR27_08590"
     CDS             8391..9011
                     /locus_tag="VLR27_08590"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HSP03542.1"
                     /translation="MRDSGSDDLDAGAFGPWLRATAAVAAGEGDADVPCGSCTACCRS
                     SYFVHVGPEDVGARRRIPPELLFPAPGRPPGHLLMGYDERGWCPMLTEHGCSIYEDRP
                     RTCRAFDCRVHAAAGTTPDGEHDEDIADRVRRWRFRLDGPDDEARWTAVQIAARHLET
                     TELPADIAPRRSADVAAAALHAHELFLDGAIPGDKDLAAALLPERP"
     gene            complement(9013..10671)
                     /locus_tag="VLR27_08595"
     CDS             complement(9013..10671)
                     /locus_tag="VLR27_08595"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HSP03543.1"
                     /translation="MARTIRLRPWQKEALERFRTKAAEGFGAEGGVDFLAVATPGAGK
                     TTFAITAALHWLHDHPDARLVVVAPTQHLKGQWASAAAAFDLALEPSWRAGDPIPADM
                     DGLVTTYQQVASNPVALAQVAHGGFVVLDEVHHAGDDRAWGDGLRVAFSGARGRLCLS
                     GTPFRSDTNPIPFVSYQWEEAVPDFEYGYGDALGDGGVVRPVHFPRIRGHMEWVGADG
                     QFHSHDFDDDLDRARANQRLRTALSAEGQWMASVLEDAHQRLLEVRRKQPDAGGLVIC
                     MDVEHARAIADLIERRWRIRPTVATSDDPMASARIARYAKSSDPWIVAVRMVSEGVDI
                     PRLRVGVFATNTTTELFFRQALGRLVRWTRGLRTQRAWMYIPDDPRLRKLADGIAEQR
                     RHSLFKRRRADDEDSLDQIDGAGLDEVDDDEQLSLFQAMSAIATGVENADDPDADDAS
                     SPEELVWEEDDGDESLEISLPPRPRRMTTVVVGRDTSLAEAKSKLRKDNAALVAHLVA
                     VTGRSHREVNGELNRLAGVNKVTEATADQLQRRLVAGERWVKTL"
     gene            10817..11932
                     /locus_tag="VLR27_08600"
     CDS             10817..11932
                     /locus_tag="VLR27_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP03544.1"
                     /translation="MIEVEEFRAGAEAWLAEQKPTAPPDWGAIMPPERRDQGMEWQQR
                     LHAAGYAGIHWPVEYGGQGLTVGHHQAWMESCADAGVPPFINMVGLVLAGGSLQLFGT
                     EDQKKAHLPETAAGERVWCQLFSEPGAGSDLASLQTRAELDGDEWVVNGQKVWCSGGR
                     ASDWGILLARTERDVPKHQGISFFLIDMRSEGVETRPLRQMTGDAEFDEVFLTDVRLP
                     VDALLGERGQGWNIAMATLTNERGHIGGMGRALTKRLEAMQALAASTGLDGPARDRLI
                     QLYSRGKAYLYMTQRQGPTASVGSSLGKLGMTGFMFDLAELRADLAGAGAMLVGPESA
                     GLLAAPGGWFGGGTSQVQRNIIGERILGLPREPRPAD"
     gene            complement(11949..12362)
                     /locus_tag="VLR27_08605"
     CDS             complement(11949..12362)
                     /locus_tag="VLR27_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSP03545.1"
                     /translation="MEATSDDPTTAAVRAANTAFYAAFEARDLDAMSDVWEHTDRVTC
                     VHPGWPILSGWASVAASWFALFDGPQRLQFIVTDEQVHVEGDTAWVTCNENLIDTGGT
                     QAVASTNVFVAGEGGWRLVHHHGSPVVQQARARNS"
     gene            12434..13261
                     /locus_tag="VLR27_08610"
     CDS             12434..13261
                     /locus_tag="VLR27_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP03546.1"
                     /translation="MITVDSTDGVRLAVHELGTPDPDARPLLLCHATGFHGRVWRALA
                     EELPGRRCLAVDFRGYGDSTEQDGELTWHGFGQDVLAVVDALDLGPVQAVGHSKGGAA
                     ILLAEEARPGTIERAVCFEPIVFPPMEGEPGPNHLAEITKKRREVFGSFEEAIANFSA
                     KPPLGSLRADVLEDYVRHGFTQQADGSVRLKASRDHESKTYETGSTHDGFAHLGEVAC
                     PVLVAHGGDADGPAQIAPLVADALPHGELRAFPELGHFGPLEDPTWFAGLVREFLDG"
     gene            13302..14141
                     /locus_tag="VLR27_08615"
     CDS             13302..14141
                     /locus_tag="VLR27_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HSP03547.1"
                     /translation="MALALPSSLSPSRLSSFTSCGLQFRFSAIDRLPEPPTVAATRGT
                     LVHAALEELFTLPAPERVPGAADACLDAAIGRLRDDPDWTGLGLDDAGEARMRDEAAA
                     LVAKYFRLEDPTTVHPVGLELKLEVEVGGVRLRGIIDRLDLVDGELVVTDYKTGRAPG
                     DAWARQRMTGVHVYSLMCERWFGRRPVRVQLLHLAEPVKIVAEPSEQSTKGLARRLEA
                     VWHAVEIACEREDFRPRPSPRCDWCAFQQWCPAFGGDPSQAVVDIERRAVEDAGQGTL
                     LEV"
     gene            14145..14732
                     /locus_tag="VLR27_08620"
     CDS             14145..14732
                     /locus_tag="VLR27_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSP03548.1"
                     /translation="MENHEVPTPRQLAAPIRRFDTTIERVFDAIRGKPLVDRVFYTAS
                     ELGNHSLIWHLLATAQGVRRDGDLAGTVRLVTIMGLESAIVNGPVKSMFRRQRPVHDG
                     PRPFQLRQPRTSSFPSGHASAAAVFAVVAGEDDPLAPVYTVLATLVGASRVHVRIHHP
                     SDVVGGAAVGVALGIALRTWWPAGQVLPRGLRRRG"
     gene            14749..>15861
                     /locus_tag="VLR27_08625"
     CDS             14749..>15861
                     /locus_tag="VLR27_08625"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSP03549.1"
                     /translation="MEYEIVRRLARGGMGVVDLARRDDGTLVALKRLALQGTPAEMRQ
                     ATVRFQRELDVLARLDHDAVVPMLDVVEEAGDVVLVMPYLPGGNLAERVRDDGPMSAD
                     EVRALAQRLLPALAAAHRLGIIHRDIKPANVLFDADGRAHLADFGVARTREITGGLTR
                     PGVVLGTPGYLAPEQARGEELTTAADIASLGATLHFAATGQSPYGTGEDHAVLLRTAR
                     AKARIDASIEPRLRRTIRSMLRAQPERRPTAAALAGGADGTRLIPALAQRRRPVVLAA
                     VAVVVLVGAALAVGLAWGGADEPEAAPEPAAETTPTEPPCSPTKYQPCGEEPAPGTDG
                     RACLDGRDDYDGLTSNGCEAEADDLDGTELVDRIAAN"
BASE COUNT         2073 a         6028 c         5510 g         2250 t
ORIGIN      
        1 gacggcaccg agcacgtgga cctgacggcg agcacgcgct cgcccgcgct caccgtgctg
       61 gacgatccac gggtgcggac ggcgaccaag gcgctcgccg cgctcgtcgc gttctacttc
      121 gtccagcagc gcctgtggcc cgcgccggcc ggggtgctgc tccagggcct cgtgctcggt
      181 gggctgaccg cgctgatctc cttcggcatc gccctgatct accgctcgaa ccggatcatc
      241 aacttctccg ccggggagct cggcgccgtc ccggcttcgc tgtcggtgct cctcatcgtc
      301 ggcccgggcc tgccctacat cctcgccgtg ccgctgggcc tcgtcgccgc gctggccctc
      361 ggcgccgtgg tcgagctggt gatcatccgg cggttcttcg acgctccccg cctcatcctc
      421 accgtcgtca ccatcggcgt gtcgagcctg ctggccgggg tcggcttcat cctcccacgg
      481 gcgttcgaca tcaccacccc gccgcagagc ttcccgtccc cgttcgactt cagcttctcc
      541 gtcggcgcca cccggttctc gggcaacgac gtggtcgcca tggtcgcggt gccgctgtgc
      601 atcgcggcgc tcggtgcctt ctaccgctac acgagcatcg gcatcgccgt gcgggccagc
      661 gccgagagct cggaccgggc cgccctgctg ggggtgccgg tcaagcgcat ccagaccatc
      721 gtctgggtca tcgccacggc gctcgccttc atcgccgtct tcctgcgggc cggcatcgtc
      781 ggcctcccct tcggccagct gctggggccg tcgatcctgg tcaaggcgct cgccgcgacg
      841 gtcatcggac gcatggagaa gatgcccctg atcctggcgg cgagcttcgc cctcggcatc
      901 ctcgagagcg ccatcatctt cgacacgggc gaggcggtcc tggtcgaccc gatcatgttc
      961 gtgatcatca cggccgccct gctgctgcaa cggtcgcgga agtcggcgcg gggtgacgag
     1021 acgtcgaact ggcaggcggc caccgacttc cgccccatcc cccgggagct gtgggccctg
     1081 cgggaggtgc gctggggcgt gcgcagcgcg ctggctgcgc tcgtggccgc caccctcgcg
     1141 ctgcccctcg tcatggacga ggccgacatc agcctcgcca ccgtcatcgt ggtcacggcc
     1201 acggtggcgg tgtcgctcgt cgtgctcacc gggtgggccg gtcaggtcag cctcggccag
     1261 atcggcttcc tcggcgtcgg tgccgctgtc ggcggctggc tcaccacgac ctggacgtgg
     1321 gacctctccc tcgccatcct cgccgccggc gtcgtcgggt cgctcacggc catggccatc
     1381 gggctgcccg ccctgcgcat caagggcctg ctcctgtcgg tgtccacgct gtcgttcgcc
     1441 ctggccgcgt cgtcgtacct gctcaaccgg agcttcttcg actggctccc ggtcgatcgg
     1501 ttcgaccggc cgctgctgtt cggtcggatc gccctggaca ccgagacccg ctacttctac
     1561 ttcgccctcg ccggcctggt gatcgccatc tacctggtga agggcctgcg ccggagccgc
     1621 accggtcggg tgctgatcgc cgtccgcgag aacgagcgag ctgcgcagtc ctacggcgtc
     1681 agcgccaccc gggcgaagct gctcggcttc gccctctccg gcttcatcgc cgcctacgcc
     1741 ggcgcgatct tcgtgcacca ccagcaggtc ttcggcatcc agccctacgc ggtcggcgag
     1801 agcctgaccg tcttcatcat ggcggtgttc ggcgggctcg gctcgatccc cggggccatc
     1861 gccggcgtcg tgctgctcga gggcatccag tacttccgcg acgccttccc cgactacatc
     1921 gccgactcgc tgcagttcct cgccagcggc gtcgggctca tcgtcgtgct ggccgtgctg
     1981 cccggcgggc tgagccaggc catctacggc atgcgcgacc ggtggctgcg caaggtcgcg
     2041 gcgcgccacg acctgcacgt cccgagcctc gtcgccgaca gccgggtcga accggcccag
     2101 atcgacgccg gcccggacga gcccgacccg gccgacaccg ccacgatcgt catggtcgag
     2161 caggcgggcg aggtggaggc cccggtcccg gtcggcgccg gcgcacccac ccgtccgcga
     2221 cgtagccggc ccctgcgcgc cggggaggac gcatgaccga caccactcgc gccgacgcgc
     2281 tcgaggcgcc cagctcgacc cagcgccggc tgctcgcggt caccggcggc gctgccacct
     2341 tcccgctggt gctgctgttc ggcctgaaca tggtcgacga gctcgaccgg gccgccttcg
     2401 gcatcctcgt gccggagatc cgcgacgcct tcggcatgag caacagcggg atcctcgggg
     2461 tcatctcgct ggtgtcgatc ctggccctgc tggcgcagcc ggtctttggc atcttcgccg
     2521 accgcacgtc gcgggtccgc ctggcgatgg gcggcgcgtc ggcgtgggcg atcttctcgt
     2581 tcctcaccgg tctggtcccg accgtggccc tgctggtgct cgcccgggtc ggcagcggca
     2641 tcggccgggc cgtgaacgac ccgacccaca actcgctgct cgccgactac tacgaacccg
     2701 acagccggat ccgcgtctac ggcttccacc gcgcggccaa ctacgtgggc cagacgctcg
     2761 gcccgctcgt cggcggtggc ctggcgttcg cgttcagctg gcgcaccccg ttcctcgtct
     2821 tctgggtgcc caccgcgatc ctcgtgctca tcgggctccg ccacctgcag gaccccatcc
     2881 gcggctacca cgagcgcaag gccggcggcg ccgacgagga ggcctcggtc accgaggagg
     2941 accaaccgtc cttcggcgag gcgtggcggc tgtgcatgca ggtccgcacg ctccgccgca
     3001 tctggtattc gctgcccgcg ctggccgtct ccctcgtcgg cttcggcagc ctctcggcga
     3061 tcttctacga ggaggtcttc gagctcaacg agctgcagcg cggcatcatc gaggccgggc
     3121 tgaacccgtt ccagatcgcc gccctgctca tcggcgtgcc cctcgccacg cgctacgccg
     3181 gcacgaaccc ggccctgctc atgcggctgc tcgccttcgt cggcgcagcc gtggccggcg
     3241 cgctggtgct gttcgcgctc agcccgaacg tggcgacggc ggtcatcgcc cacatcgtgg
     3301 tggtggcgcc gctcgccctg ctcctgcccg gcatctacgc catcctgtcg ctggtcatcc
     3361 cgccccgcgc ccgggggttg gggttcggca tcggctcgct ctacgtcatc cccggcctgc
     3421 tgctgctccc catcgtgggc gccgtggccg acgacgtcgg cgcccgccag gccatgatcg
     3481 tgatggtccc ggtgttcctg ctcggctcgg gcatcatctc ctcggccggg gccttcgtga
     3541 acgaagacgt cgccaaggtc cgggccgcca ccgccgcgca gtcggcggtc cgcctggccc
     3601 gggagcgggg cgagacgaag ctgctgctcg tcaaggacct cgacgtggcc tacgggaacg
     3661 tccaggtcct cttcggggtc gacttcgagg tggaccaggg cgagatcgtg gcgctgctcg
     3721 gcaccaacgg cgccggcaag tccaccctgc tcaaggccat ctcagggctc gtggaacccg
     3781 ccgggggggc cacgatcttc gacggccggg acatgacgca cgccaccccg caggagatcg
     3841 cggcccgggg tgtcatccag gtgccggggg gcaagggcgt gttccccggc ctcaccgtcg
     3901 ccgagaacct caagatcgct ggctggctgt accagaagga caccgagtac ctggaggcgg
     3961 ccacggagcg ggtgctggag ttcttcccga tcctgcggga gcgctgggac gacaaggccg
     4021 gcaacatgtc cggcggcgag cagcagatgc tcaccctcgg ccaggccttc atcgccaagc
     4081 cgcgcctgct catgatcgac gagctgtcgc tcgggttggc gcccgtgatc gtggagcagc
     4141 tgctggggat cgtgcgagcc atccgcgacc agggcaccac cgtcatcgtc gtcgagcagt
     4201 cggtgaacgt ggcgctcacc ctggccgaga ccgcctactt catggagaag ggcgagatcc
     4261 ggttccacgg gcccaccaag gagctgctcg agcgccccga cgtcctgcgg tccgtgttcc
     4321 tcgagggcgc tggtgccgtg agcggatccg acaccggcga cgccgaccag gacaacccct
     4381 tcgccgacct cgacctgcgg acgccggcgc tcgcggcccg gccacgccac gtcgagccgg
     4441 aggcgtccgg cccgatcctc tcggtcgccg gcgtcggcat cagcttcggc ggcgtccgag
     4501 cggtccacga cgtcagcttc gacctgggcc ggaacgagat cctcggcatc atcggcccga
     4561 acggcgcagg caagacgacc ctgttcgacc tcatctccgg ctacctcgtc accgacaccg
     4621 gtcgcatcga gctcgacggc ttcgacatca cgcacacctc ggccgacgcc cgggcccggc
     4681 tcggcatggg gcgctccttc caggacgccc gcatgttccc gtccctcacc gtcaccgaga
     4741 acatcgccgt cgccctcgag cggcacctgg agatgcgcga ccccatcgcc gcggcgctca
     4801 acctgccggc ggtcgcggac gccgagcggc gggtcggtga gcgggtcgac gagctcgtcg
     4861 acctcatgaa cctgggcgcg ttcgccgaca agttcgtctc cgagctgtcc accgggagcc
     4921 ggcgcatcgt cgacctcgcc tgcgtcctcg cccaccgacc cgacgtgctg ctcttcgacg
     4981 agccgtcgtc cggcatcgcc cagcgcgaga ccgaggcgct cggtccgctc ctgctgcgca
     5041 tccgcgaggc caccggcgcc agcctgatcg tgatcgagca cgacatgccc ctgatctcca
     5101 gcatctcgga ccggatgctg gccctcgagc tgggccagaa ggtcgtcgag gggccacctc
     5161 gccgggtcat cgaggacccc cgggtggtgg cgtcctacct cggcaccacc gacgaggtga
     5221 tcaaccgctc ggacgcagcc accgaacgca ccgacagcac cgtcgaccaa ggggagaccc
     5281 agacgtgaac gacaagacca tcgcggacat ccgggcgttc ctcacgcggt tcggccccta
     5341 cctgtgcctg gtcggagcga tcgccgccgt catggtgttc ctgccgggtg acgacgaccg
     5401 cacgaacgtg ggcaccacca gcgccttcgg cgacttcgag gacggcgact ccacgacggt
     5461 cgccgccggc cgggacggga cgacccgcac gaccaccggc gcgaccggcg cgacggcgac
     5521 cggggccggc acgggctcga ccggtgcctc ctcgggcggc ggaggcggcg gcgggggcgg
     5581 cagcggcgac accggcgatg gcggtggtgg tggcggcggc gaggccggtg gcggtgagca
     5641 ggccgccccg ggcgagatgg tggccaactg cgatctcggc accggacgca tcgcggtccc
     5701 ctcccactac gccccgccct gcgttccggc gttcgggggc gacaacggcg gggagaccta
     5761 ccagggcgtc accggggaca agatcaagct ggccgtcatg ctggtggagg gcgatgcagc
     5821 ctccgaggcc gtgctcagcg ccgcgggcgc caacgacagc gaggaggccg tgcgccagca
     5881 gtacctcgac tacgtcgact actactcgac ccactacgag acctacgggc gcgagatcga
     5941 ggtcaccttc tacaacggca gcggcaccgc caccgacgac gccgcgggac gtgccgacgc
     6001 cgtgcgcatc gccaacgacg gcaacttcgc cgtcctgaac gccccgaaca acgcgttcgt
     6061 cgacgagctg gtcgcgcgcg gcgtcatgtg catgtgcacg gtctccctgc cgggcagctt
     6121 ctacgagcag cgtgcgccgt tcgtctggac gacgctgatg gactcgttcc agggctacat
     6181 ccaccgggcc gagttcatcg gcaagcgcct cgccggcggc aaggccgagt tcgccggcga
     6241 ccccaccctc gccctcgccg accgggtgtt cggcctcgcc tactacgacg acgtccagca
     6301 gtcgtaccgg ccggggatcg acttcttcac cgagcacctc cgggacagct ggggcatctc
     6361 gctggccgcg gtggtgggct accggggcta ccccgacgtg gccgagagcc aggaggccgc
     6421 ccgtcccctg atccagaaga tgatcgccaa cgacgtcacg agcgtgatct gcgcgtgcga
     6481 ccccttcgct cccatcttct tcacgcagga ggccacgagg cagaacttcc gacccgagtg
     6541 gatcatcacc ggatcggcgc tcaccgacac cgcgttcttc gcccgcacct acgaccagac
     6601 ccagtgggcc aacgcgttcg gcatctcgtt cctcacggtc ggcctgcccg acgagctcgg
     6661 cgacgactac cgcaagctcc agtggcacca cggccggccg ccggccgcgg aggcgaccta
     6721 cggcgtcatc gacggcccga cccagatctt cttcgacggg gtgcacctcg ccggcccgca
     6781 gctcgacccg gggacgttcc gcgacgggat gttccgcgcc ggatcccggg cgacgggtct
     6841 gaccacggca gcgtcgtcgt cattcgggac cgaggtctgg gggcgtcctg actacgccgc
     6901 cctggacgac atcaccatga tctggtggga tccccaggcc cagggcgagg acgagaccgg
     6961 gcagtacggc gtgggcctgt accagtacat ggacggcggc cgtcgctacc tccccggcca
     7021 gcagccggaa gggccgctgg cggcgttcga ccgggccggg accgtcagcg cctacgacgg
     7081 gtacccggag ggtgaggcac cgccggacta cccgccgccg ggctgatcac ggtcagccgg
     7141 cgggctcgag cagtcgcagc tcgcccccct cgacctcctc gaggccgtcg agcagctcga
     7201 tgagcaggtc cggggccacc gtgccccggg cccgcaggaa ccggccctcg cccaggtcga
     7261 cgatcaccgt cggaccaccg aggccgtagg tgaggacccc cgccccggcc gcgacgtcga
     7321 cgtcctcgcc ctgcagggcg gggaacgggt cgacgttgcc gacggtgccg ccctggtcga
     7381 ggacgatgac atcgggcccg tcgacgaaga cgtcgctgat gtaggtcagc cgctgcggca
     7441 gccgatcgat ctcggtgaag ttctcggcct gcgccgggac gacggcgaag cggccgtgcc
     7501 ggctgaaccc ggggggcgtg ctcggctcga agaaccggcc cgggacccgg ctcccgggtt
     7561 cggtctcggc gacgaagccc ccgccctcgt ccacggagag cgtcggatcc cccgtctcga
     7621 acgagacccc gtcgaggtcc gggctgatct ccacctcggt ggcgatgcgc cgcagcagga
     7681 cctcgccctc ggacaccagg acctcctcca gcaccagtcc gctggcatcg atgcaggtgt
     7741 cggcgtactc gtcgggtccg gtggcggggc tgaggtcgcc ggtcgccagc gtcgtcgcgc
     7801 tgcgcacgac ctggcaggtg cgctccgcga cgcgccgccg ctcgacgagc tcggcccgcc
     7861 cgtcctcgac cgctcggacc agggcgccgg cgatgcgcac atcggatgtc gccgaccccg
     7921 ggggcgacgc cacgaccacg gcgtcgccgt cctcgccgtc ggcccggaag cccccgaagg
     7981 cggagacctg cacggcatcc ggttccccgt cgggcgtggc cccggggtac acctcgagcc
     8041 ggctgtccca cggacggcgc acccacagcc ggtcgctggt cacgatcagc tcgtcgcctc
     8101 cgtggccctc cacccggtag tcgatgcggt agctctccgg cgcgggctcc gggtcgagca
     8161 gctgcgtggg atcggcgtcg acgagctcgg acccgctccg tggtccggca gcggcgtcgt
     8221 cgccggcgcg cagcgcgagc agcgcgcctc cggccacgag cagcgccagg acacccgcct
     8281 gcgcggcgcg ccggtgccgc acggcccatc ccatcgcgat ccccagcacg gcgtcgcagc
     8341 gtaccgacgg ccccgacccg tggtcgccct ccgccgggta ccgtcgaacc gtgcgcgact
     8401 ccgggagcga cgacctcgac gccggcgcgt tcgggccctg gctgcgggcc accgccgccg
     8461 tcgcagccgg cgaaggcgac gccgacgtcc cgtgcgggag ctgcaccgcc tgctgccgct
     8521 cgtcgtactt cgtgcacgtg ggccccgagg acgtcggcgc ccggcgccgc atcccgcccg
     8581 agctgttgtt ccccgccccc ggtcgcccgc ctggccacct cctgatgggc tacgacgagc
     8641 ggggctggtg cccgatgctc accgagcacg gttgctcgat ctacgaggac cgaccccgga
     8701 cgtgccgagc gttcgactgc cgcgtccacg ccgccgccgg gaccacgccc gatggcgagc
     8761 acgacgagga catcgccgac cgcgtccgcc gatggcggtt ccggctcgac ggtcccgacg
     8821 acgaggcgcg ctggaccgcg gtgcagatcg cagcccggca cctggagacc accgagcttc
     8881 ccgcggacat cgcgccccgg cgctcggccg acgtggccgc cgcggcgctc cacgcccacg
     8941 agctgttcct cgatggtgcg atcccgggcg acaaggacct cgccgccgcc ctcctgcccg
     9001 aacgcccctg agctagagcg tcttgaccca gcgctcaccg gcgaccaggc ggcgctgcag
     9061 ctggtccgcc gtggcctcgg tgaccttgtt gaccccggcg aggcggttga gctcgccgtt
     9121 gacctcgcgg tggctgcgac cggtgaccgc cacgaggtgg gcgaccaggg cggcgttgtc
     9181 cttgcgcagc ttggacttgg cctcggcgag cgaggtgtcc cgaccgacga ccacggtcgt
     9241 catccgccgg ggccggggcg gcagcgagat ctcgagggac tcgtcgccgt cgtcctcctc
     9301 ccacaccagc tcctcgggcg aggaggcgtc gtcggcatcg ggatcgtccg cgttctcgac
     9361 cccggtggcg atggccgaca tggcctggaa cagcgacagc tgctcgtcgt cgtccacctc
     9421 gtccaacccg gccccgtcga tctggtcgag cgagtcctcg tcgtcggcgc ggcggcgctt
     9481 gaacagcgag tggcggcgct gctcggcgat gccgtcggcc agcttccgca ggcgcgggtc
     9541 gtcggggatg tacatccacg cccgctgggt gcgcaggccg cgggtccagc ggacgaggcg
     9601 gcccagggcc tgacggaaga acagctcggt ggtggtgttg gtggcgaaca ccccgacgcg
     9661 caggcggggg atgtccacgc cctcggacac catccgcacc gccacgatcc acgggtcgct
     9721 cgacttcgcg tagcgggcga tgcgggcgga cgccatcgga tcgtccgagg tcgccaccgt
     9781 ggggcggatg cgccagcggc gttcgatcag gtcggcgatg gcccgggcgt gctccacgtc
     9841 catgcagatc accagaccgc cggcatcggg ctgcttgcgg cggacctcga ggagacgctg
     9901 gtgggcgtcc tcgagcaccg acgccatcca ctggccctcc gccgacagcg cggtgcggag
     9961 gcgctggttg gcgcgggccc ggtcgaggtc gtcgtcgaag tcgtgggagt ggaactggcc
    10021 gtccgcgccg acccactcca tgtgccccct gatgcggggg aagtgcacgg gccggaccac
    10081 gccgccgtcg ccgagggcgt cgccgtagcc gtactcgaag tccgggaccg cctcctccca
    10141 ctggtaggag acgaagggga tcgggttcgt gtcggaccgg aacggcgtgc cggacaggca
    10201 gagtcggccc cgggcgccgg agaacgcgac ccgcagcccg tcgccccagg cccggtcgtc
    10261 gccagcgtgg tggacctcgt cgaggacgac gaagccgccg tgggccacct gggccagcgc
    10321 caccgggttg gaggcgacct gctggtaggt ggtgaccagg ccgtccatgt ccgccgggat
    10381 cgggtcgccg gcccgccacg acggctcgag ggccaggtcg aaggcggccg ccgcgctggc
    10441 ccactggccc ttgaggtgct gggtcggagc gaccacgacg agccgggcgt cggggtggtc
    10501 gtgcagccag tgcagggcgg cggtgatggc gaaggtcgtc ttgccggcgc ccggcgtggc
    10561 cacggccagg aagtccacgc caccctcggc gccgaagccc tccgccgcct tggtccggaa
    10621 ccgctcgagg gcctccttct gccaggggcg gagtcggatc gtgcgggcca tggggcgtcc
    10681 gatcctagga gcgacccccc gaggtccagc gcatccacag acgaactggc cgccagatcg
    10741 tcacagcggg cttctgtgac gaggtggctg ccagttcgtt cccgtgggaa cggtcgttgc
    10801 cagtatcgtc gccggcgtga tcgaggtcga ggagttccgg gccggcgccg aagcatggct
    10861 ggccgagcag aagcccacgg ccccgccgga ctggggggcg atcatgccgc ccgagcggcg
    10921 ggaccagggg atggagtggc agcagcggct gcacgccgcc ggctacgcgg gcatccactg
    10981 gccggtcgag tacggcggcc aggggctcac cgtcgggcac caccaggcgt ggatggagag
    11041 ctgcgccgac gccggcgtgc cgccgttcat caacatggtc ggcctggtgc tggccggcgg
    11101 gtcgctgcag ctgttcggca ccgaggacca gaagaaggcg cacctgccgg agaccgcggc
    11161 cggcgagcgg gtctggtgcc agctgttctc cgagccgggt gccggctccg acctggcctc
    11221 cctgcagacc cgggccgagc tcgacggcga cgagtgggtc gtcaacgggc agaaggtgtg
    11281 gtgctcgggt ggtcgggcgt cggactgggg catcctgctc gcccgcaccg agcgcgacgt
    11341 ccccaagcac cagggcatct ccttcttcct gatcgacatg cgcagcgagg gcgtggagac
    11401 ccgcccgttg cgccagatga ccggcgacgc cgagttcgac gaggtcttcc tgaccgacgt
    11461 ccgcctgccg gtcgacgccc tcctcggcga gcggggccag gggtggaaca tcgcgatggc
    11521 caccctcacc aacgagcggg gccacatcgg cggcatgggc cgcgccctga ccaagcgcct
    11581 cgaggcgatg caggcgctcg ccgcgtcgac ggggctcgac ggcccggcgc gcgaccgcct
    11641 catccagctc tactcccgcg gcaaggcgta cctgtacatg acccagcgcc aggggcccac
    11701 cgcgtcggtc gggtcgtcgc tcggcaagct cggcatgacc gggttcatgt tcgacctcgc
    11761 cgagctgcgt gccgacctcg ccggcgccgg cgccatgctc gtgggccccg agtccgccgg
    11821 cctgctcgcc gcacccggcg gctggttcgg cggcggcacc agccaggtgc agcgcaacat
    11881 catcggcgag cgcatcctcg gcctgccccg ggagccccgc ccggccgact gacgggtccg
    11941 gacggacgct acgagttccg ggctcgggcc tgctggacga cgggcgaccc gtggtggtgc
    12001 acgaggcgcc aaccaccctc gccggcgacg aagacgttgg tggacgccac cgcctgcgtg
    12061 ccgccggtgt cgatgaggtt ctcgttgcag gtgacccacg ccgtgtcgcc ctcgacgtgg
    12121 acctgctcgt cggtgacgat gaactgcagc cgctggggac cgtcgaagag ggcgaaccag
    12181 gacgccgcca ccgacgccca ccccgagagg atgggccagc ccgggtgcac gcacgtgacc
    12241 cggtcggtgt gctcccagac gtcggacatg gcatcgaggt cacgcgcctc gaacgctgcg
    12301 tagaaggcgg tgttggcggc ccgcaccgcg gcggtggtgg ggtcgtcgct cgtggcttcc
    12361 atccgggcga cgaccgtagc cgtgctcccg cccatgtcca ccgtgcccgc ggccggtgcc
    12421 aggatggccg gcgatgatca ccgtcgactc caccgacggg gtgcgcctcg ccgtccacga
    12481 gctgggcacc cccgaccccg acgcccgtcc cctgctgctg tgccacgcca ccggcttcca
    12541 cggacgggtc tggcgcgcct tggccgagga gctccccggc cgccggtgcc tggcggtcga
    12601 cttccgcggc tacggcgact cgaccgagca ggacggcgag ctgacctggc acggcttcgg
    12661 ccaggacgtg ctggcggtcg tcgacgccct cgacctcggc ccggtgcagg cggtcggcca
    12721 ctccaagggc ggcgccgcca tcctcctcgc cgaggaggcc cgtcccggca ccatcgagcg
    12781 ggcggtgtgc ttcgaaccca tcgtgttccc gccgatggag ggcgagccgg gtccgaacca
    12841 cctcgccgag atcaccaaga agcggcgcga ggtcttcggc tcgttcgagg aggccatcgc
    12901 caacttctcc gccaagcccc cgctcggttc gctgcgagcc gacgtgctcg aggactacgt
    12961 gcgccacggc ttcacgcagc aggccgacgg cagcgtccgc ctcaaggcga gccgcgacca
    13021 cgaatccaag acctacgaga ccggatcgac ccacgacggc ttcgcccacc tcggcgaggt
    13081 cgcctgcccg gtgctggtcg cccacggcgg tgacgccgac ggtccggccc agatcgcccc
    13141 cttggtcgcc gacgccctcc cccacggcga gctgcgcgcc ttcccggagc tgggacactt
    13201 cggcccgctc gaggacccga cgtggttcgc cggcctggtc cgggagttcc tggacgggta
    13261 ggggacccgg cgctgtcgga cccccggcgt acgctgcctc catggccctg gcgctcccca
    13321 gctcgctgtc gccgtcccgg ctgtcgagct tcacgtcgtg cggcctgcag ttccggttct
    13381 cggccatcga ccgcctgccg gagcctccca ccgtcgccgc cacccgcggc acgctggtgc
    13441 acgccgccct cgaggagctg ttcacgctgc cggcgcccga gcgcgtgccc ggcgcagccg
    13501 acgcctgcct ggacgcggcg atcggacgcc tgcgcgacga cccggactgg accggtctcg
    13561 gcctggacga cgccggcgag gcacgcatgc gcgacgaggc ggccgcgctg gtggcgaagt
    13621 acttccgcct ggaggacccc accaccgtcc acccggtcgg cctggagctg aagctcgagg
    13681 tcgaggtcgg cggcgtgcgc ctgcggggca tcatcgaccg gctcgacctg gtcgacggtg
    13741 agctcgtcgt caccgactac aagacgggcc gggccccggg cgacgcctgg gcccgccagc
    13801 gcatgaccgg ggtgcacgtc tactccctca tgtgcgagcg gtggttcggc cgccgcccgg
    13861 tccgggtgca gctgctccac ctggccgagc cggtgaagat cgtggccgag ccctcggagc
    13921 agtccaccaa gggcctggcc cgccggctcg aggccgtgtg gcacgcggtc gagatcgcct
    13981 gcgagcgcga ggacttccgg ccgcggccct ctccccgctg cgactggtgc gcgttccagc
    14041 agtggtgccc ggccttcggg ggcgacccgt cccaggccgt cgtcgacatc gagcgccgcg
    14101 ccgtcgagga tgccggccag ggcaccctcc tcgaggtctg accggtggag aaccacgagg
    14161 tccccacgcc gcggcagctc gcggcaccca tccgccgctt cgacaccacc atcgagcggg
    14221 tgttcgacgc catccggggc aagcccctgg tcgaccgcgt cttctacacc gcctccgagc
    14281 tcggcaacca cagcctcatc tggcacctcc tcgccaccgc acagggcgtg cggcgcgacg
    14341 gcgacctggc gggcaccgtc cggctggtca cgatcatggg cctcgagtcc gccatcgtga
    14401 acgggccggt gaagtcgatg ttccggcgcc agcgcccggt gcacgacggt ccccgcccgt
    14461 tccagctccg ccagccccgc acctcctcgt tcccctccgg gcacgcctcc gccgccgccg
    14521 tgttcgccgt cgtggccggc gaggacgacc cgctcgcgcc cgtctacacc gtgctcgcca
    14581 ccctggtggg cgcctcgcgg gtgcacgtgc gcatccacca cccctccgac gtggtcggcg
    14641 gcgctgcggt cggcgtcgcc ctgggcatcg ccctgcgcac gtggtggccg gccggccagg
    14701 tcctgccgcg cgggctccgc cgccgcggct gatccctacc ctgggccggt ggagtacgag
    14761 atcgtccgcc gtctggcgcg tggaggcatg ggggtcgtcg acctcgcccg gcgcgacgac
    14821 ggcaccctcg tcgccctgaa gcgcctggcc ctgcagggga ctccggccga gatgcgccag
    14881 gccacggtcc ggttccagcg ggagctcgac gtgctcgccc gcctcgacca cgacgccgtc
    14941 gtgccgatgc tcgacgtggt ggaggaggcc ggcgacgtcg tcctcgtcat gccctacctg
    15001 ccgggcggga acctggccga gcgcgtgcgc gacgacgggc cgatgtccgc cgacgaggtg
    15061 cgggcgctgg cccagcggct cctgcccgca ctcgccgccg cccaccgcct cgggatcatc
    15121 caccgggaca tcaagccggc caacgtgctc ttcgacgccg acggccgggc ccacctcgcc
    15181 gacttcggcg tcgcccgcac ccgggagatc accggtggcc tcacccggcc cggcgtggtg
    15241 ctcgggaccc ccggctacct cgcccccgag caggcccggg gcgaggagct gaccaccgcc
    15301 gccgacatcg ccagcctcgg agccaccctg cacttcgccg ccaccggcca gagcccgtac
    15361 ggcaccggcg aggaccatgc ggtgctcctg cggaccgcgc gggccaaggc gcgcatcgac
    15421 gcgtcgatcg agccccgcct gcggcgcacc atccggtcca tgttgcgggc gcagcccgag
    15481 cgccgtccca cggccgcggc gctggcgggc ggtgccgacg ggacgcgcct gatcccggcg
    15541 ctggcccagc gccggcggcc ggtggtgctc gcggccgtcg cggtcgtcgt cctggtcggc
    15601 gccgcgctcg ccgtcggtct ggcctggggc ggcgccgatg agccggaggc cgcacccgag
    15661 ccggctgccg agaccacgcc gaccgaaccg ccgtgctcac cgacgaagta ccagccctgt
    15721 ggcgaggaac ccgccccggg caccgacggg cgggcgtgcc tcgacgggcg ggacgactac
    15781 gacgggctca ccagcaacgg ctgcgaggcc gaggccgacg acctggacgg caccgagctg
    15841 gtcgaccgca tcgccgccaa c
//
LOCUS       DATISU010000208        19619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_801221, whole genome shotgun sequence.
ACCESSION   DATISU010000208 DATISU010000000
VERSION     DATISU010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 19619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19619
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801221"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1032)
                     /locus_tag="VLR27_08630"
     CDS             complement(145..1032)
                     /locus_tag="VLR27_08630"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSP03550.1"
                     /translation="MRTPATGLQLTTQQLEYLVAIDRHDTWGEAAAAVGVSPSALSQG
                     IAELERRLGMQLFERDGRRRVPSRDHAPVLRYAEATVAATRDLARWLAARRTGTTGVV
                     RLGMIDAAATHHFRDVLRAHRRQRPDVDLLLAVDPSRPLLERLGRAELDLVVCVESDD
                     LPGGITTQPLLDDPLSVLAPGDSLDAPPADWGPWVTFPAGSHTRDVIADSLRRAGAGF
                     EVVAESNQPEVLREMVLLGVGWTVLPLAGLGPVDGLRVVRRDLVVRRLVLARRADARP
                     DPAVDELAGALLAGVRTGS"
     gene            1102..2403
                     /locus_tag="VLR27_08635"
     CDS             1102..2403
                     /locus_tag="VLR27_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HSP03551.1"
                     /translation="MTARDIAPATGKLGVLLPGLGAVASTFIAGVLQARQSGVAPIGS
                     LSQMAHIRLGEREEGRNPLIKDFVPLASLDDIVFGGWDPISPNALEAARTAGVLEERD
                     LAPISAEMEGIVAMDAVFDQSWVSRLEGTRVKTGASKMELAEQLIADIESFRTENGCD
                     RLVIVWCASTEAYRDVTEVHQSVEAFEQGLRDSDPMISPSQIYAYAALISGVPFANGA
                     PNLSTDLPCMVELALREGVPIGGKDFKTGQTLMKTILAPGFKARMLGLRGWYSTNILG
                     NRDGEVLDDPENFKSKEVSKLGVIDTILQPDAYPDLYGNVDHVVRINYYPPRGDNKEG
                     WDNIDIFGWMGYPMQIKVNFLCRDSILAAPLVLDLALFFDLAARAGESGVQEWLSFYF
                     KAPMVANDELLPEHDIFIQQIKLKNTLRTWMGEEPVTHTEQ"
     gene            2414..2950
                     /locus_tag="VLR27_08640"
     CDS             2414..2950
                     /locus_tag="VLR27_08640"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HSP03552.1"
                     /translation="MSDDQTAAQADIIPQTLVGISFPDIYRAQEFLTAVTRLKVNGSL
                     VLEDAVIVVKDDDGKTVVRETIDPQPGRSAISGGMWTGLFGLLLGGPVGWAAGTAIGA
                     GAGALTAKVVDLGISDEWVGWFREAVKPGTATVAVLASEIVEDHLVAEVHRFAGSELV
                     YANFDAEMIGRLERALEI"
     gene            3020..3448
                     /locus_tag="VLR27_08645"
     CDS             3020..3448
                     /locus_tag="VLR27_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSP03553.1"
                     /translation="MGLAMTVEEREQFLAGVHVGVLAVERPDGPPLAVPVWYDYRPGG
                     ELWVLTAEDSVKGKLLAQARRFSLCAQDETPPFYRYVSVEGPITAIRAADEEDDSRPM
                     AHRYLGPDIGDQYVETSDEQSLKFCMQPERWWSVDYSKLT"
     gene            3503..4264
                     /locus_tag="VLR27_08650"
     CDS             3503..4264
                     /locus_tag="VLR27_08650"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSP03554.1"
                     /translation="MTINDLQGQVAVVTGGNGGIGLGFAEGCARAGAAVAVWARNTEK
                     SAAAVETIQAHGVDAIAVACDVGDEGSVAAATAETVERLGRIDVCVANAGTSGGGQLQ
                     DLSLEEWRRVMSINLDGAFLTMRDCARQMIDQGEGGAIVAISSTSAIHGAAANPHYGA
                     SKTALLGLVRASAVGLARHKIRVNSVLPGWTRTEMAEGGYQDERFREVTTKRTPVRRW
                     ATGADYEKLAAYLCDKSVDWHTGDQIVADGGYTIF"
     gene            4268..4852
                     /locus_tag="VLR27_08655"
     CDS             4268..4852
                     /locus_tag="VLR27_08655"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HSP03555.1"
                     /translation="MDEGLRTGADGIVRCWWCGDDPLYVDYHDHEWGRLTRDEREVFE
                     LLALEGFQAGLAWITILRKGEAFRTAFDGFDVDTVAGYGEPEVERLLGDAGIVRHRGK
                     IEATVDLARLSQQLRDEGSSLSELAWSFAPEPRPGPLGALADIPAHTPESAALSKALK
                     ARGARFVGPTIVYAFMQSAGMVDDHLIGCHRAGS"
     gene            complement(4824..6008)
                     /locus_tag="VLR27_08660"
     CDS             complement(4824..6008)
                     /locus_tag="VLR27_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSP03556.1"
                     /translation="MTAGLSDAPGGGDGGEPAPADTHTVVDVDLRSIVTLLVVALVAL
                     GLLEVLQQTPSMITKIVVGIVIALALDPVVARVRDRLGGSSRGIAVAIVGTGLGMALL
                     GVLLVLGPAAIDQAGSLRSDLPATIEDAYTWPVIGERIAEAELAVRVDDAIERLPAEL
                     DDEQITRYAEDLLGGLLTTVVVLVTAIAVMLDGESMVQRIRNLFTGERQEHLDGIGRV
                     VYRTFGNYFAGSLFVAILNGLVVLTVALSLGVPLAPLAGLWSMLTNLIPQIGGFLGGS
                     FLVTLALTQGPVAAVIALVVFLVYQNLENNIIQPAVVGKAVDLTPPTTMLAALLGGAM
                     AGVPGALIATPIVGAVKVLYLRDARDGSLDGRTVGEVGRTDEGVFRRVLGRITNRRGG
                     NR"
     gene            6029..7117
                     /locus_tag="VLR27_08665"
     CDS             6029..7117
                     /locus_tag="VLR27_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSP03557.1"
                     /translation="MPPTPWMTGGGTRRHRTVRISTRTIVLAVGVVLALLVFRSAFVA
                     GQRVLGWAAACAVTAVFVAPLVSWLGRWIPRIAAVLLTFVVIGVAAVALVFGTFDNLD
                     REIRSLQRAAPDATERLEERDDEIGAAMRDLELSSRIEVFLDEVDERVGSGSDALAEN
                     ALTVPVFFVNMILTIFLLLYGPGIIRGGLSLIGDEERRRSATRVLEDATVQARRTLAA
                     MLGQGVLVGLTVGVVGSLLSLPAPVVLGLVAGVAATLPDVGILLGVLPTVALTAGLQS
                     GRAAVAILAAAMVVQAVEALHVRRWVDHWGVEVGPSIVWIVVLLGYELHGIGMAFFGV
                     AFAVFVLAVIDQVPRGREPEGELAPARP"
     gene            7130..8035
                     /locus_tag="VLR27_08670"
     CDS             7130..8035
                     /locus_tag="VLR27_08670"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSP03558.1"
                     /translation="MPTLSRRTAGILATTAGVSIWGASSTMVKSIDGIDATGISFYRL
                     VLGAVLLVTVHLARGGRITWSMLRISALGGVAFGLDIVLFFSALRETSVANATVIGAL
                     QPILLAPIGVRLFGEQLGKRVVVWSIVAVAGTAVVVLGATGVPEWSLRGDLLAVGALG
                     SWTLYFVASKQARRQLGTLEYFTGLSVVACVVVAPYALLVGADLSPAGTSDWVTIVVV
                     TVFSGALGHVLLNWSHEHVPLQVVSLLTLLVPLVATVGAVTFLDESVSALQWFGMAVV
                     VAALAVVVLERPAQEPREVGEPVPT"
     gene            8069..8524
                     /locus_tag="VLR27_08675"
     CDS             8069..8524
                     /locus_tag="VLR27_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03559.1"
                     /translation="MDLHEQIREALELQAIEADGPGAAQVLQTAILEHTGIAVGDALL
                     DAAAVALRRMVLETEETMVAPELMTRLALDGAVPEDRIELLLTTMTMAAATAGGIRPS
                     VEALIGGEGLEDSMFGLWLGLLTIVRVVAMALDMTEAELVEDVLVALEP"
     gene            8573..8998
                     /locus_tag="VLR27_08680"
     CDS             8573..8998
                     /locus_tag="VLR27_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03560.1"
                     /translation="MRVVIGPVSAASALAWLDYADEVIEELDAMAPGECFTTPEVLEV
                     FRGYVTSWRASAVGDELLWERDIPSEQVEYDMHAFQRVAEVLAERAEREGTAAPEEGE
                     DFYLALLQGVLSALEAEGPASAAFAQHLGEFWPGRDPLS"
     gene            complement(9008..9622)
                     /locus_tag="VLR27_08685"
     CDS             complement(9008..9622)
                     /locus_tag="VLR27_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HSP03561.1"
                     /translation="MRRLPIYGLDIETDTTVDGLDPNVSRIVAVALSTDLGDELFDGP
                     EDELLAELDLRLGELAPGVLATWNGSAFDLPFIADRAVRWRLRLGLQLVADPSIRHRH
                     DPLPGHDCCYRAAWHGHGHLDAYRVYRDDVGPALRISCSLKSIARFVGLAPLEVDRER
                     IHDLSREALHAYAASDARLARVLAERRWATAARRIDQVPQALAG"
     gene            complement(9709..10440)
                     /locus_tag="VLR27_08690"
     CDS             complement(9709..10440)
                     /locus_tag="VLR27_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03562.1"
                     /translation="MPDTSDSPGPDPVDDRPRIPTPEEAATYRPAHDLFVALLDVVSE
                     ATLGRPLRPELKISPAGLLRGEIDLVKLELPAYAVAGLVIDRFIVRAEHVRVQPGLPP
                     VLVAEPVGFTAVVSQENVDRWTRDLHLPFRLKLTEEGVVTTTGFGGVRMSEIVTMPAV
                     VGGFVQLQPTKASLLGVPTTLVRFLRGYLPLPPLPRSARLDDVEASDGELSLSFVIDR
                     FEQPLTPDVSERIRQLLQLPLPGLR"
     gene            complement(10485..10784)
                     /locus_tag="VLR27_08695"
     CDS             complement(10485..10784)
                     /locus_tag="VLR27_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HSP03563.1"
                     /translation="MDDVVALGSWAGQDWMAKAACAGKTRLFFAPLAERPQARARREA
                     KAAELCRACPSRTACRDFARERREFGYWGGENEEERTRAGFPVPNPVGARRRAAS"
     gene            complement(10947..12353)
                     /locus_tag="VLR27_08700"
     CDS             complement(10947..12353)
                     /locus_tag="VLR27_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HSP03564.1"
                     /translation="MTPDPALVVDAPVEPMLPDLDPSTRTQVVLDTSALVADPEGVLL
                     GYPGCDLIVPLTVIEELDGLKKRLDSVGMSARDVLRRIERIRVDGGGDLETPQPLPDG
                     ATLRVALNGVQVGLLREHGLDPDKPDNRIIGTALGQHYDAGRSVRLVSNDTGMRVKAA
                     TFGLDSCEHDPLDSARQGDGENGWRELQVEPAVVDAIYAGDLAGPGTEHVLAALRVNE
                     FGVLHAGSQSALVRRHAHGLELVGNDHHAWDLRPRNKEQRFALDLLLDDDVKVVALDG
                     PAGTGKTLTAVAAALQQVVEERRYSRICVYRPVVPVGKADVGYLPGTLDEKLDPWMAA
                     ITDAVYALTGPDHTGQRQREDAAEVLDEIRARGQLTMESVTHLRGRTLHSSFVIIDES
                     MNLSPQVGKTILTRIAADSKVVFTGDTSQIDAAFLSEQTNALTALVTAFAGQDCFGHV
                     RLLRGERSPLAELAARLL"
     gene            complement(12491..13246)
                     /locus_tag="VLR27_08705"
     CDS             complement(12491..13246)
                     /locus_tag="VLR27_08705"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HSP03565.1"
                     /translation="MAQARIGCSGWSYKDWRGPVYPEKAPARTWFAHYAERFDTVELN
                     NTFYRLPTPEKVDGWREQAPPGFTYAAKVGQFCTHRKKLKDPEIWLPRHLDRIRRLGD
                     HLGPNLFQLPPSWNRDVPRLEAVLELLPDDIRWAVELRDPSWVHDDTFDALARRGVAL
                     CLHDLIADHPQVLTTDWTYIRYHGPDALNHPYNGRYGPERMARDAERLTVWLDDGVDV
                     YAYFNNDWYGHAVTDALDLRSHLGDWAPQMTAV"
     gene            13286..13720
                     /locus_tag="VLR27_08710"
     CDS             13286..13720
                     /locus_tag="VLR27_08710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HSP03566.1"
                     /translation="MAACTFCRIVAGDAPAHVVLDDDVAVGFLDIRPLFPGHVLLVPR
                     DHVEVLPDLPPTLVGPLFERAQRIARAMVEVLGAQGSFVAMNNTVSQSVPHLHVHVVP
                     RTKGDGLRGFFWPRTGYDSDEHAADVAARLRDWLAAENFVSE"
     gene            complement(13752..13970)
                     /locus_tag="VLR27_08715"
     CDS             complement(13752..13970)
                     /locus_tag="VLR27_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03567.1"
                     /translation="MLEDGIYDALVFDADEAEGGGVAVELTILAGEHKGAVVSVVSHD
                     WTGDALDLLGIPATLVVTDGRPQVTFEP"
     gene            14010..14915
                     /locus_tag="VLR27_08720"
     CDS             14010..14915
                     /locus_tag="VLR27_08720"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSP03568.1"
                     /translation="MAVIVVTGANSGIGRSTAVHLAAAGHTVHGAMRDLGKGAKLQDE
                     ATAAGATVHPLQLDVSDDASVRDGIQSVLDAEGRIDVLVNNAGLGYNKVVEEIDIAEG
                     KEVFDVNYWGVVRCTQAVLPAMRAQGAGTIVNVSSVAGRLAAIAQAVYASSKWALECL
                     SENMAQELAPFGIRVKVIEPGVTRTALLAKHQELHPESPYADAYRRMFAFYATGIEAN
                     VRPSEVAEVVQQAIEDDSARLRYVCAYAGEELAGGREGVSDEDWLALGAAANDDEYFE
                     RFERAFGLDIRAGDFRTDEQRAAGL"
     gene            14926..15513
                     /locus_tag="VLR27_08725"
     CDS             14926..15513
                     /locus_tag="VLR27_08725"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HSP03569.1"
                     /translation="MSLRGHLLVAEATLDDPNFARTVVYLIEHSEGGALGLVLNRPGE
                     VPVHEAVPTWAPYVDGVGVVFVGGPVSPEGAICLARCRSAGPQESLFAGTPEHEPTAL
                     FKPITTTIGAVDLHGDPSDAPGGITALRVFAGYAGWSGGQLELELESGGWYVLEAEDD
                     DVFTDAPERLWRDVLRRQRGSLRSAAYFPLDPRWN"
     gene            15542..16405
                     /locus_tag="VLR27_08730"
     CDS             15542..16405
                     /locus_tag="VLR27_08730"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HSP03570.1"
                     /translation="MSHAVTDRPAPPMGEHDPIARAMLEESLSGIGLAAAGANVIMQL
                     SMLPVGHGVASSRVDSGRVDKHPIKRARTTLAYLAVALLGTEDERSAMRSEVDRQHRQ
                     VRSRPGDPVRYNAFDTELQLWVAACLYKGTQDIERVAGIHRSEEEAERIYRYCARLGT
                     TLQVRPEQWPADRAAFARWWDEKVDQIEMDDLTRTYLQGIAKADFVGRPWSTLLGPLA
                     ELQAVGFLPERFRAELGLPWNHRHQRAFDAMMKTWAAVDRHLPGPVRRLPFNAYLWDT
                     RRRIRRGKPIV"
     gene            16432..16947
                     /locus_tag="VLR27_08735"
     CDS             16432..16947
                     /locus_tag="VLR27_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP03571.1"
                     /translation="MAAELEMVDPEDAGPLAALKAETFVETFAEDNDPDHVRAHVARE
                     FTAEAVRPSLADDATTTWWLIDDDVPVGFLKVNRGRSQTEPDLDDGLEVEQIYVLAAH
                     HGRGHGRRLMDHALDTARAEGFPFIWLGVWEHNRRAMAMYEHLGFVPFGDHTFLFGDE
                     EQRDVLMRRDL"
     gene            complement(16928..18268)
                     /locus_tag="VLR27_08740"
     CDS             complement(16928..18268)
                     /locus_tag="VLR27_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HSP03572.1"
                     /translation="MESTVYADRVVRQREQIPEVYGDVDFSIVPERYDADATVDDERF
                     ASMRRAIERVIDEPDTMRVILDATMTGDRLADAYAALIPEHGFRRLVELIETACSKGL
                     DAVDDPPPELVALIESMEATPDWVDMDLVRRGARAERVPMATATPFAIRGAFLATFLN
                     QYAALPMTMTGTLSDEAAAKRVFETASFFTATTMPGALDRHGKGFEAAAKVRLMHSMV
                     RFHLLRSGEWDVSTYGIPIPQVDQMPAGLIGVFLMSAQVLRKGRTEFTEAERAQVELS
                     RYRCFLLGLPEELLGETPQEIVDLLMARHVSLREAYDDEICGSLVRGTMAAELFDVSS
                     VAGRTHAWLENGFSRFVLIKSFLGGDAERAERMGIDFGPREKVAAGVAAGVIAALTAY
                     YRLGMRLPVVGGLVDRQLDARLAHLLDSYGHADFVTEPHRYKVAAPPEVTDRDA"
     gene            complement(18356..18613)
                     /locus_tag="VLR27_08745"
     CDS             complement(18356..18613)
                     /locus_tag="VLR27_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03573.1"
                     /translation="MAKAKKPSSKWSEGSADVDDLAPRERLAHEIVSQHGDLLPSVER
                     IMAAELTEEQGVVALTAFRDSLDNPGDPNRDPRVSIANAAG"
     gene            complement(18684..19469)
                     /locus_tag="VLR27_08750"
     CDS             complement(18684..19469)
                     /locus_tag="VLR27_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HSP03574.1"
                     /translation="MSTPDLPTPSPPGASTGVSRDLPVPTSALAIAAHPDDVEFGAGA
                     TLAKWAAAGCVVHHLVCTDGSKGTWDPNQDPAELVATRQVEQRAAAKALGGTGEVAFL
                     GWPDGELDSSLRQRWQVAYWIRRLRPAVVLGHDPWKRYRLHPDHRHAGLLAVEGIVAA
                     RDPLFFPEQELEHHRPDVALLWEADEPDHVEAVEDRDVQAKLEALRAHESQFQTTMGV
                     ALDDPAADEQWDAFAERIRGRLAEWGRFGGCDLGEGFKKLDRL"
BASE COUNT         2713 a         6822 c         7274 g         2810 t
ORIGIN      
        1 cgaaggatcc ggtgggctcg acgtactgct cgatgccgtc ggagcggagt cgcttgtccc
       61 gctcgaggcg gtacttctcg cggagggcgt ctggatcgat cgtggcggcc acgtgcgcga
      121 cagaaccaga tcttgacccg cgggtcaaga tccagttcgc acgcccgcca gcagggcgcc
      181 ggccagctcg tccaccgcgg ggtccggccg ggcgtcggcg cggcgggcca gcaccaggcg
      241 gcgcaccacg aggtcgcggc ggacgacccg caacccgtcg acgggtccga ggccggccag
      301 cggcagcacc gtccagccca cgccgagcag caccatctcg cgcaggacct cgggctggtt
      361 ggactcggcg acgacctcga agccggcgcc cgcccggcgg agggaatcgg cgatgacgtc
      421 acgggtgtgg gagccggcgg ggaaggtgac ccacggtccc cagtcggccg ggggtgcgtc
      481 gagcgagtcg ccgggggcca gcacgctgag cgggtcgtcg agcaggggct gcgtggtgat
      541 gccgcccggc aggtcgtcgg actcgacgca cacgacgagg tcgagctcgg cccgtcccag
      601 gcgctcgagc agcggccggg acgggtcgac ggcgaggagc aggtcgacgt cgggacgctg
      661 gcgccggtgg gcacgaagga cgtcgcggaa gtggtgggtg gcggcggcgt cgatcatccc
      721 gagccggacg acgccggtgg tcccggtgcg ccgggccgcc agccagcggg ccaggtcgcg
      781 ggtggcggcg acggtggcct cggcgtagcg cagcacgggt gcgtggtcgc gggagggcac
      841 gcgacgtcga ccgtcgcgct cgaacagctg catgccgagg cggcgctcga gctcggcgat
      901 gccctgggag agcgcggagg gggacactcc gacggctgca gccgcctcgc cccaggtgtc
      961 gtgacggtcg atggccacca ggtactcgag ctgctgggtg gtgagctgga gaccggtcgc
     1021 cggggtgcgc accacccgca cgatagttca gcattcctga acagatgttg cgttctgttc
     1081 accgttgttc atactggagc catgaccgca cgagacatcg cgcccgccac gggcaagctc
     1141 ggcgtgctcc tcccggggct cggcgccgtt gcatccacct tcatcgccgg cgtcctgcag
     1201 gcgcgccaga gcggcgtggc ccccatcggc tcgctcagcc agatggccca catccgcctc
     1261 ggcgagcgcg aggagggccg caacccgctg atcaaggact tcgtccccct cgccagcctc
     1321 gacgacatcg tcttcggcgg gtgggacccg atctcgccca acgcgctcga ggccgcccgc
     1381 accgccggcg tgctcgagga gcgcgacctg gccccgatct cggccgagat ggagggcatc
     1441 gtcgccatgg acgcggtgtt cgaccagagc tgggtctccc gcctcgaggg cacccgggtc
     1501 aagaccggcg cctcgaagat ggagctggct gaacagctca tcgccgacat cgagagcttc
     1561 cggaccgaga acggctgcga ccgcctcgtc atcgtgtggt gcgcctcgac cgaggcctac
     1621 cgcgacgtca ccgaggtcca ccagagcgtc gaggccttcg agcagggcct gcgcgacagc
     1681 gacccgatga tcagcccgag ccagatctac gcctacgccg cgctgatctc cggcgtcccc
     1741 ttcgccaacg gcgcgcccaa cctgtccacc gacctgccgt gcatggtaga gctggcgttg
     1801 cgcgagggcg tgccgatcgg cggcaaggac ttcaagaccg gccagacgct gatgaagacg
     1861 atcctggcgc cgggcttcaa ggcccggatg ctgggcctgc gcggctggta ctccaccaac
     1921 atcctcggca accgcgacgg cgaggtgctg gacgacccgg agaacttcaa gtccaaggag
     1981 gtctccaagc tcggcgtgat cgacacgatc ctgcagccgg acgcctaccc ggacctctac
     2041 ggcaacgtcg accacgtcgt gcggatcaac tactacccgc cccgcggcga caacaaggag
     2101 ggctgggaca acatcgacat cttcgggtgg atgggctacc cgatgcagat caaggtcaac
     2161 ttcctgtgcc gcgactcgat cctggccgcc cccctcgtgc tcgacctggc cctcttcttc
     2221 gacctcgccg cccgcgccgg tgagtcgggc gtgcaggagt ggctgagctt ctacttcaag
     2281 gccccgatgg tcgcgaacga cgagctgctc cccgagcatg acatcttcat ccagcagatc
     2341 aagctgaaga acacgctgcg cacgtggatg ggcgaagagc ccgtcaccca cacggagcag
     2401 tagaaagagc ctcgtgagcg acgatcagac ggccgcgcag gccgacatca tcccccagac
     2461 gctggtgggc atctccttcc cggacatcta ccgggcccag gagttcctga ccgccgtcac
     2521 ccggctcaag gtgaacggtt cgctggtgct ggaggacgcg gtcatcgtgg tcaaggacga
     2581 cgacggcaag accgtcgtcc gcgagaccat cgacccccag ccgggtcgct cggccatctc
     2641 cggtggcatg tggaccggct tgttcggtct gctcctcggt gggccggtcg gttgggccgc
     2701 gggcaccgcc atcggtgccg gcgccggcgc gctcacggcg aaggtcgtcg acctcggcat
     2761 ctcggacgag tgggtcggct ggttccgcga ggcggtcaag ccgggcaccg ccacggtggc
     2821 cgtcctcgcc agcgagatcg tcgaggacca cctcgtggcc gaggtgcacc gcttcgccgg
     2881 ctccgagctg gtctacgcca acttcgacgc cgagatgatc ggtcgcctcg agcgcgccct
     2941 cgagatctga ccctccggag cgggcccgcc ggcaccgtga caggatgagc ccagtgccgt
     3001 cgactgcagg aggtgccgca tggggctcgc gatgaccgtc gaggagcggg agcagttcct
     3061 ggccggcgtg cacgtggggg tgctggcggt ggagcgcccc gacgggccgc cgctcgccgt
     3121 ccccgtctgg tacgactacc gacccggcgg cgagctgtgg gtgctcaccg ccgaggactc
     3181 ggtgaagggc aagctgctgg cgcaggcccg tcggttctcg ctgtgcgccc aggacgagac
     3241 gccgcccttc taccgctacg tcagcgtcga gggcccgatc accgccatcc gcgccgccga
     3301 cgaggaggac gactcccggc cgatggcgca ccggtacctc ggccccgaca tcggtgacca
     3361 gtacgtcgag acctcggacg agcagagcct caagttctgc atgcagcccg agcgctggtg
     3421 gagcgtggac tactccaagc tcacctagcg gcccgttccg cagccgcgtc cagaacgggg
     3481 tggatggcta cgctccgccg ccgtgacgat caacgatctg cagggccagg tggccgtggt
     3541 gaccggcggc aacgggggca tcgggctcgg cttcgccgag ggctgcgccc gggccggtgc
     3601 ggccgtggcg gtgtgggcgc gcaacaccga gaagtcggcg gctgcggtcg agaccatcca
     3661 ggcccacggc gtggatgcca tcgcggtggc ctgcgacgtc ggcgacgagg ggtcggtcgc
     3721 cgccgccacc gcggagacgg tcgaacggct cggccgcatc gacgtctgcg tggccaacgc
     3781 cggcaccagc ggtggtggcc agctgcagga cctctccctc gaggagtggc gccgggtgat
     3841 gtccatcaac ctcgacggcg cgttcctcac catgcgcgac tgcgcccgcc agatgatcga
     3901 ccagggcgag ggtggcgcca tcgtggcgat ctcctccacc tccgccatcc acggggccgc
     3961 cgccaacccg cactacggcg cttcgaagac cgcgctgctc ggcctcgtcc gggcatcggc
     4021 ggtcgggctc gcccgccaca agatccgggt gaactccgtg ctgcccggct ggacccgcac
     4081 cgagatggcc gagggcggct accaggacga gcgcttccgc gaggtcacca ccaagcggac
     4141 ccccgtccgc cggtgggcca ccggtgcgga ctacgagaag ctcgccgcct acctgtgcga
     4201 caagtccgtc gactggcaca ccggcgacca gatcgtggcc gacggcggct acaccatctt
     4261 ctagatcgtg gacgagggac tgcgcaccgg cgccgacggc atcgtgcggt gctggtggtg
     4321 cggcgacgac cccctctacg tcgactacca cgaccacgag tggggccggc tgacccggga
     4381 cgagcgcgag gtgttcgagc tcctggcgct ggagggcttc caggccgggt tggcgtggat
     4441 caccatcctg cgcaagggcg aggcgttccg caccgccttc gacggcttcg acgtcgacac
     4501 ggtggcgggc tacggggaac ccgaggtcga gcgcctcctc ggcgacgccg gcatcgtccg
     4561 gcaccggggc aagatcgagg ccaccgtcga cctggcccgg ttgagccagc agctgcgcga
     4621 cgagggctcc tccctgagcg agctggcctg gtccttcgcg cccgaacccc gtccggggcc
     4681 gctgggcgcg ctggccgaca tcccggccca caccccggag tcggcggcgc tgagcaaggc
     4741 gctcaaggcg cgtggcgccc gcttcgtcgg cccgacgatc gtctacgcgt tcatgcagtc
     4801 ggccgggatg gtggacgacc acctcatcgg ttgccaccgc gccggttcgt gatccgcccg
     4861 aggacccggc ggaacacccc ctcgtcggtc cggccgacct cgccgaccgt gcggccgtcg
     4921 agggagccgt cccgggcgtc tcgcaggtag agcaccttga ccgcgccgac gatcggcgtg
     4981 gcgatcagcg ctccgggcac accggccatg gcgccgccga ggagggcggc cagcatcgtc
     5041 gtcggcggtg tcaggtcgac ggccttgccg accactgcgg gctggatgat gttgttctcg
     5101 aggttctggt acacgaggaa cacgaccagc gcgatcacgg cggccaccgg gccctgggtg
     5161 agggcgaggg tgacgaggaa cgagcctccg aggaacccgc cgatctgggg gatcaggttg
     5221 gtgagcatcg accacagccc ggcgaggggg gcgagcggga cgccgagcga gagcgcgacc
     5281 gtgagcacga cgaggccgtt gaggatggcc acgaacaggg agccggcgaa gtagttgccg
     5341 aacgtgcggt agacgacccg gccgatcccg tcgaggtgct cctggcgctc gccggtgaac
     5401 aggttccgga tgcgctgcac catcgactcg ccgtcgagca tgacggcgat cgcggtcacg
     5461 agcaccacga ccgtggtgag gagcccgccg agcagatcct cggcgtagcg ggtgatctgc
     5521 tcgtcgtcga gctcggcggg caggcgctcg atggcgtcgt cgacccgcac ggccagctcg
     5581 gcctcggcga tgcgctcgcc gatcacgggc caggtgtacg cgtcctcgat ggtggccggc
     5641 aggtcggagc gcaggctgcc ggcctggtcg atggccgccg ggccgagcac gagcagcacg
     5701 ccgagcagcg ccatgccgag gccggtgccg acgatggcca cggcgatgcc ccggctggag
     5761 ccgccgaggc ggtcgcgcac gcgggccacg accgggtcga gtgccagcgc gatcacgatc
     5821 ccgaccacga tcttggtgat catcgacggg gtctgctgca gcacctcgag gaggccgagc
     5881 gccaccagcg cgacgacgag gagcgtcacg atcgagcgga ggtcgacgtc gaccaccgtg
     5941 tgcgtgtcgg cgggcgcggg ctcgcctccg tccccgcccc cgggcgcatc actgaggccg
     6001 gcggtcaccc tcggtaccgt acggctcgat gccgccgaca ccgtggatga ccgggggcgg
     6061 gacccgccgg caccgcacgg tccgcatcag cacccggacc atcgtgctgg cggtgggcgt
     6121 cgtcctcgcc ctgctcgtgt tccgcagcgc cttcgtcgcc ggccagcgcg tcctcggctg
     6181 ggctgctgcc tgcgccgtga ccgccgtgtt cgtggccccg ctggtgagct ggctcggccg
     6241 ctggatcccc cggatcgccg cggtgctgct gaccttcgtc gtcatcggcg tggctgcggt
     6301 cgcgctcgtc ttcggcacgt tcgacaacct cgatcgcgag atccgctccc tgcagcgggc
     6361 cgcgccggac gccacggagc gcctcgagga gcgcgacgac gagatcggtg ccgccatgcg
     6421 cgacctcgag ctgtccagcc ggatcgaggt cttcctcgac gaggtcgacg agcgggtggg
     6481 cagcggcagc gacgccctcg ccgagaacgc cctcaccgtt ccggtgttct tcgtgaacat
     6541 gatcctcacg atcttcctgc tcctctacgg cccgggcatc atccgcggcg gcctctccct
     6601 gatcggcgac gaggagcggc gccgctcggc gacccgggtg ctggaagacg ccacggtgca
     6661 ggcccgtcgc accctggccg ccatgctcgg gcagggcgtg ctcgtgggtc tgacggtggg
     6721 ggtcgtgggc tctctgctgt cgctgcccgc gcccgtggtc ctgggtctgg tcgccggcgt
     6781 ggccgccacc ctccccgacg tcgggatcct cctcggggtg ctgcccaccg tggcgctcac
     6841 cgccggcctc cagagcggtc gggccgccgt ggcgatcctg gcggcggcga tggtcgtgca
     6901 ggccgtcgag gccctgcacg tccggcgctg ggtcgaccac tggggcgtgg aggtcgggcc
     6961 ctccatcgtg tggatcgtgg tgctgctcgg ctacgagctg cacgggatcg gcatggcgtt
     7021 cttcggcgtg gccttcgcgg tgttcgtcct ggcggtgatc gaccaggttc cccgcggacg
     7081 ggaaccggaa ggggagctgg caccggcgcg gccgtagggt gctgcggcca tgcccacgct
     7141 cagccggcgc accgccggga tcctcgcgac gaccgccggc gtcagcatct ggggtgcgtc
     7201 gtcgacgatg gtcaagtcga tcgacggcat cgacgccacc ggcatcagct tctaccgtct
     7261 cgtgctcggc gccgtcctcc tcgtcaccgt gcacctcgct cggggcgggc ggatcacctg
     7321 gtcgatgctg cggatcagcg ccctcggcgg cgtcgccttc ggcctcgaca tcgtcctgtt
     7381 cttcagcgcg ctgcgagaga cgtcggtggc caacgccacg gtcatcggcg cgctgcagcc
     7441 gatcctgctc gcccccatcg gcgtgcggct cttcggcgag cagctcggga agcgcgtcgt
     7501 ggtgtggtcg atcgtcgcgg tcgccggcac ggcggtggtc gtgctgggtg ccaccggcgt
     7561 gcccgagtgg agcctgcgcg gcgacctgct cgcggtcggg gcgctcggat cctggaccct
     7621 ctacttcgtc gcctccaagc aggcccgccg ccagctcggg accctcgagt acttcaccgg
     7681 cctctccgtg gtcgcctgcg tcgtggtcgc cccctacgcc ctgctcgtcg gcgccgacct
     7741 gagcccggcc ggcaccagcg actgggtgac gatcgtcgtg gtcacggtct tctccggggc
     7801 gctcggccac gtgctgttga actggtccca cgagcacgtg ccgctccagg tcgtgtcgct
     7861 gctgaccctg ctggtgccgc tggtggccac cgtcggcgcg gtcacgttcc tcgacgagtc
     7921 ggtcagcgcg ctgcaatggt tcggcatggc ggtggtggtc gccgcgctgg cggtcgtggt
     7981 cctcgagcgc cccgcccagg agccgcgcga ggtcggcgag ccggtgccga cgtagccagc
     8041 gcggccggcc gctcgtatcg tcggcgcgat ggacctccac gagcagatcc gcgaagccct
     8101 cgagctgcag gcgatcgagg cagacggtcc cggcgcggcc caggtgctgc agacggccat
     8161 cctcgagcac accggcatcg cggtgggcga cgcgctcctc gatgccgccg ccgtcgcgct
     8221 gcgccgcatg gtgctcgaga ccgaggagac catggtcgcg ccggagctga tgacgcgcct
     8281 cgccctcgac ggtgccgtcc ccgaggaccg catcgagctg ctcctgacca ccatgacgat
     8341 ggcggccgcc acggccgggg ggatccgccc gtcggtggag gccctcatcg gcggcgaggg
     8401 cctggaggac tccatgttcg ggctctggct cggcctcctc accatcgtgc gggtggtcgc
     8461 gatggcgctg gacatgaccg aggccgagct ggtcgaggac gtgctggtgg ccctcgagcc
     8521 ctgagtgtcg cccgggtggt gcgccgcccg gcggtgtgcc aacgtcgcgc gggtgcgagt
     8581 ggtcatcggt ccggtcagtg cagcgagcgc cctcgcgtgg ctcgactatg ccgacgaggt
     8641 catcgaggag ctcgacgcca tggccccggg ggagtgcttc accaccccgg aggtgctcga
     8701 ggtgttccgc ggctacgtga cctcctggcg ggcatcggcc gtcggcgacg agctgctgtg
     8761 ggagcgggac atcccgtccg agcaggtgga gtacgacatg cacgcgttcc agcgcgtggc
     8821 cgaggtgctg gcggagcggg ccgagcgcga ggggacggct gcgccggagg agggcgagga
     8881 cttctacctc gcgctgctgc agggcgtgct gtcggcgctg gaggccgagg gcccggccag
     8941 cgccgccttc gcccagcacc tgggcgagtt ctggccgggg cgcgacccgc tgtcctgagc
     9001 gggtcgctca gccggcgagg gcctgcggga cctggtcgat ccggcgggcg gccgtggccc
     9061 agcggcgctc ggcgaggacg cgtgcgagcc gggcgtccga cgcggcgtac gcgtgcagcg
     9121 cctcgcggga caggtcgtgg atccgctcgc ggtcgacctc gagcggtgcc agcccgacga
     9181 agcgggcgat ggacttgagc gagcaggaga tgcgcagcgc cgggccgacg tcgtcgcggt
     9241 agacccggta ggcgtcgagg tgcccgtgcc cgtgccacgc cgcccggtag cagcagtcgt
     9301 ggccggggag cgggtcgtgg cggtggcgga tgctgggatc ggccaccagc tgcagcccga
     9361 ggcggagccg ccagcggacg gcccggtcgg cgatgaaggg caggtcgaag gccgagccgt
     9421 tccaggtggc gagcacgccg ggggcgagct cgccgaggcg gaggtcgagc tcggcgagca
     9481 gctcgtcctc ggggccgtcg aacagctcgt cgccgaggtc ggtcgacagg gcgaccgcca
     9541 cgatccggga cacgttcggg tcgagtccgt cgacggtcgt gtcggtctcg atgtcgagcc
     9601 cgtagatcgg aagtcgtcgc aatccgctac cccccgggtg gtgctgggcc gatggtaggg
     9661 acgcgccgcg cccgaccggt ggatccccgt gcggtgtgac cgccggcgtc agcgcaggcc
     9721 ggggaggggc agctggagga gctggcggat ccgctcggac acgtccggcg tgagcggctg
     9781 ctcgaagcga tcgatgacga aggacaggga cagctcgccg tcgctggcct cgacgtcgtc
     9841 gagccgggcg ctgcggggca gtgggggcag gggcaggtac ccccgcagga agcggaccag
     9901 ggtcgtgggg accccgagca gggaggcctt cgtcggctgc agctgcacga acccgccgac
     9961 gacggccggc atggtgacga tctcgctcat gcgcacgccg ccgaagccgg tggtggtgac
    10021 cacgccctcc tcggtgagct tcagccggaa cgggaggtgc aggtccctgg tccagcggtc
    10081 cacgttctcc tgggagacca cggcggtgaa gcccaccggc tcggccacga gcacgggcgg
    10141 caggccgggc tgcacccgca cgtgctcggc gcggacgatg aagcggtcga tgaccaggcc
    10201 ggccaccgcg taggcgggca gctccagctt gaccaggtcg atctcgcccc ggagcagccc
    10261 ggcaggcgag atcttgagct ccggacggag cgggcgaccg agggtggcct cggagacgac
    10321 gtcgagcagc gcgacgaaca ggtcgtgcgc cggccggtac gtggccgctt cctccggggt
    10381 ggggatccgc ggccggtcgt cgacggggtc aggaccgggg gagtcggagg tgtcgggcaa
    10441 cggaagggat gctacggacc tccgccgcga ttcggacgga gggcctagct ggcggcgcgg
    10501 cgtcgggccc cgacggggtt ggggacgggg aagccggcgc gggtgcgctc ctcctcgttc
    10561 tcaccgcccc agtagccgaa ctcgcggcgc tcgcgggcga agtcccggca cgccgtgcgg
    10621 gacggacagg cccggcacag ctcggcggcc ttggcctccc ggcgggcccg ggcctgcggg
    10681 cgctccgcga gcggcgcgaa gaacaggcgg gtcttgcccg cgcacgcggc cttggccatc
    10741 cagtcctgcc cggcccagct ccccagtgcg acgacgtcgt ccacagccct ctccccatgc
    10801 tctcgccggt ctgacccggc tggtccctct cggtgcgccg accacggcag tgggctgtac
    10861 tgcgcgtggt cgaacgacca ccaaactact gccggtgttt cggctggtac agagggtgag
    10921 gagaatgcgg tgaatgtggc atttgcctag agcagccggg ccgccagctc ggcgagcggc
    10981 gagcgctcgc cgcggagcag ccggacgtgg ccgaagcagt cctggccggc gaacgcggtg
    11041 accagcgccg tgagcgcgtt ggtctgctcg gagaggaagg ccgcatcgat ctggctggtg
    11101 tcgccggtga agaccacctt ggagtcggcg gcgatgcggg tgaggatcgt cttgccgacc
    11161 tggggcgaca ggttcatgga ctcgtcgatg atgacgaagc tcgagtgcag cgtgcggccc
    11221 cgcaggtggg tgaccgactc catggtgagc tggccccggg cccggatctc gtcgagcacc
    11281 tcggccgcgt cctcgcgctg gcgctggccg gtgtggtccg ggccggtgag ggcgtagacc
    11341 gcatcggtga tcgccgccat ccacgggtcg agcttctcgt cgagggtgcc cggcaggtag
    11401 ccgacgtcag ccttcccgac cgggacgacg ggccggtaca cgcagatccg ggagtagcgc
    11461 cgctcctcca ccacctgctg gagggcggcg gcgacggcgg tgagggtctt gccggtgccg
    11521 gcggggccgt cgagggcgac gaccttgacg tcgtcgtcga ggagcaggtc gagggcgaag
    11581 cgctgctcct tgttccgggg gcgcaggtcc caggcgtggt ggtcgttgcc caccagctcg
    11641 aggccgtggg cgtggcggcg caccagcgcc gactggctgc cggcgtgcag cacgccgaac
    11701 tcgttgaccc gcagcgcggc gagcacgtgc tcggtgccgg gcccggcgag gtcgccggcg
    11761 tagatggcgt cgacgaccgc cggctccacc tggagctcgc gccacccgtt ctccccgtcg
    11821 ccctgccggg cgctgtcgag cgggtcgtgc tcgcaggagt cgaggccgaa cgtggccgcc
    11881 ttcacccgca tgccggtgtc gttggagacg aggcgcaccg accgcccggc gtcgtagtgc
    11941 tggccgaggg cggtgccgat gatgcggttg tcgggcttgt cgggatccag gccgtgctcg
    12001 cgcagcaggc ccacctgcac gccgttgagg gcgacgcgca gcgtggcgcc gtcgggtagc
    12061 ggctggggcg tctcgaggtc gccgccgccg tcgacccgga tccgctcgat gcggcgcagg
    12121 acgtcgcggg ccgacatgcc cacggagtcg aggcgcttct tgagcccgtc gagctcttcg
    12181 atgaccgtga gcggcacgat gaggtcgcag cccgggtagc ccagcagcac cccctccggg
    12241 tcggccacga gggccgaggt gtcgagcacg acctgggtgc gggtgctcgg gtcgaggtcg
    12301 gggagcatcg gctccacggg cgcgtcgacg acgagtgcgg ggtccggggt catgcagcct
    12361 cctgggggcg cagggcacgg ctgagcgccg gggccgagga tcggccgggt cgtcgtgcca
    12421 aggaacggac gaacactgct gtcgatcatg gtcccacccg cgccggcagg cgcggatggt
    12481 gtgccggtct tcacaccgct gtcatctgcg gggcccagtc cccgaggtgc gaccgcaggt
    12541 cgagcgcgtc ggtgacggcg tgtccgtacc agtcgttgtt gaagtacgcg tagacgtcga
    12601 cgccgtcgtc caaccagacg gtgagccgct cggcgtcacg ggccatccgc tcgggtccgt
    12661 agcggccgtt gtaggggtgg ttcagcgcgt cggggccgtg gtagcggatg taggtccagt
    12721 ccgtggtgag gacctggggg tggtcggcga tcaggtcgtg caggcacagc gccacgccgc
    12781 ggcgggcgag cgcgtcgaac gtgtcgtcgt ggacccagct cggatcgcgc agctccacgg
    12841 cccagcggat gtcgtcgggg aggagctcga ggaccgcctc gagccgcggc acgtcgcggt
    12901 tccagctcgg cggcagctgg aacaggttgg ggccgaggtg gtcgccgagg cggcggatgc
    12961 ggtcgaggtg gcgcggcagc cagatctcgg ggtccttcag cttcttgcgg tgcgtgcaga
    13021 actgaccgac cttggccgca tacgtgaacc cgggcggggc ctgctcccgc cagccgtcga
    13081 ccttctcggg cgtggggagc cggtagaagg tgttgttcag ctcgacggtg tcgaagcgct
    13141 cggcgtagtg ggcgaaccag gtccgggccg gggccttctc cgggtacacc ggcccccgcc
    13201 agtccttgta ggaccacccc gagcagccga tgcgcgcctg tgccacgtgc tccccgtacc
    13261 cggcctcagc gggcaggatg ccgtgatggc cgcgtgcacg ttctgtcgca tcgtcgccgg
    13321 cgacgcaccg gcccacgtcg tgctcgacga cgacgtggcc gtgggcttcc tcgacatccg
    13381 cccgctgttc cccggccacg tgctgctcgt gccccgggac cacgtcgagg tcctgcccga
    13441 cctgccgccg acgctcgtcg ggccgctgtt cgagcgggcc cagcgcatcg ccagagcgat
    13501 ggtcgaggtg ctcggtgccc agggcagctt cgtggcgatg aacaacacgg tcagccagag
    13561 cgtcccccac ctccacgtcc acgtcgtgcc ccgcacgaag ggtgacgggc tgcgtgggtt
    13621 cttctggccc cgcaccggct acgactccga cgagcacgcc gccgacgtgg ccgcccgcct
    13681 ccgcgactgg ctcgcagctg agaatttcgt gtctgagtga cagcagctgt cacccaggca
    13741 cgagattccg gtcagggttc gaaggtcacc tgcggtcggc cgtcggtgac cacgagggtt
    13801 gcggggatgc cgagcaggtc gagtgcgtcg cccgtccagt cgtgggacac gacggagacg
    13861 accgcgccct tgtgctcgcc agcgaggatc gtgagctcga cggccacgcc gccgccctcg
    13921 gcctcgtcgg cgtcgaacac cagggcgtcg tagatcccgt cctccagcac ggcgtcggag
    13981 gctagggccg tggtgccaga ctcggcgcca tggccgtcat cgtcgtcaca ggtgcgaact
    14041 ccggcatcgg ccggtccacc gccgtccacc tcgccgccgc cggccacacc gtccacgggg
    14101 cgatgcgcga cctcggcaag ggggccaagc tccaggacga ggccacggcc gccggcgcca
    14161 cggtgcaccc cctccagctc gacgtctccg acgacgcctc ggtgcgcgac ggcatccagt
    14221 ccgtgctcga cgccgagggc cgcatcgacg tcctcgtcaa caacgccggc ctcgggtaca
    14281 acaaggtcgt cgaggagatc gacatcgccg agggcaagga ggtgttcgac gtcaactact
    14341 ggggcgtcgt ccgctgcacc caggccgtgc tgccggccat gcgggcgcag ggcgcgggca
    14401 cgatcgtcaa cgtctcctcc gtcgccggcc gcctcgccgc catcgcccag gccgtgtacg
    14461 cctcgtccaa gtgggcgctg gagtgcctca gcgagaacat ggcccaggag ctggcgccgt
    14521 tcggcatccg ggtgaaggtg atcgaaccgg gcgtgacccg caccgcgctg ctggccaagc
    14581 accaggagct ccaccccgag tcgccctacg cggatgcgta ccgccgcatg ttcgccttct
    14641 acgccaccgg catcgaggcc aacgtccgcc cgtcggaggt cgccgaggtg gtccagcagg
    14701 ccatcgagga cgactcggcc cgcctgcgct acgtgtgcgc ctacgccggt gaggagctgg
    14761 ccggcggccg ggagggcgtg agcgacgagg actggttggc gctcggtgct gcggcgaacg
    14821 acgatgagta cttcgagcgc ttcgagcggg cgttcggcct cgacatccgg gccggcgact
    14881 tccgcaccga cgagcagcgg gccgcaggcc tctgacggca cgagcatgag cctgcgggga
    14941 cacctgctcg tcgccgaagc gacgttggac gatcccaact tcgcccgcac cgtcgtctac
    15001 ctgatcgagc actccgaggg aggcgcgctg ggcctggtgc tcaaccgtcc cggcgaggtg
    15061 ccggtgcacg aggcggtgcc gacgtgggct ccctacgtcg acggggtggg cgtggtgttc
    15121 gtgggcgggc cggtgagccc ggagggggcg atctgccttg cccggtgccg cagcgccggg
    15181 ccgcaggaga gcctgttcgc cggcacgccg gagcacgagc cgaccgcgct cttcaagccg
    15241 atcaccacga cgatcggggc ggtcgacctc cacggtgacc cctccgacgc gcctggcggc
    15301 atcaccgccc tgcgggtgtt cgccggctac gccggctggt cgggcgggca gctcgagctg
    15361 gagctcgaga gcgggggttg gtacgtgctc gaggccgagg acgacgacgt gttcaccgac
    15421 gcgcccgagc ggctgtggcg cgacgtcctg cgtcgccaac gcggatcgct gcgctcggcg
    15481 gcgtacttcc cactcgaccc tcgctggaac tgaccgccgt cgcgggacat aggatggatc
    15541 tgtgtcccac gccgtgaccg atcgacctgc gccgccgatg ggcgagcacg accccatcgc
    15601 ccgggcgatg ctcgaggagt cgctgtcggg catcggcctc gccgctgccg gcgccaacgt
    15661 catcatgcag ctctcgatgc tgcccgtcgg ccacggcgtg gcctccagcc gggtcgacag
    15721 cggccgggtg gacaagcacc cgatcaagcg cgctcgcacc acgctggcct acctggccgt
    15781 ggcgctcctc ggcaccgagg acgagcggtc ggccatgcgc agcgaggtcg accgccagca
    15841 ccgccaggtg cgctcccgcc ccggtgaccc ggtgcgctac aacgcgttcg acaccgaact
    15901 gcagctgtgg gtcgccgcct gcctgtacaa gggcacccag gacatcgagc gcgtggccgg
    15961 catccaccgc tccgaggagg aggccgagcg gatctaccgc tactgcgccc gtctcggcac
    16021 cacgctgcag gtgcgccccg agcagtggcc cgccgaccgg gccgccttcg cgcggtggtg
    16081 ggacgagaag gtcgaccaga tcgagatgga cgacctgacc cgcacctacc tgcagggcat
    16141 cgccaaggcc gacttcgtcg gtcgaccgtg gtcgacgttg ctcggaccgc tggccgagct
    16201 gcaggccgtc ggcttcctgc cggagcgctt ccgtgccgag ctgggactgc cgtggaacca
    16261 ccgccaccag cgggccttcg acgcgatgat gaagacctgg gccgccgtcg accgccacct
    16321 gcccggcccc gtccggcggc tcccgttcaa cgcctacctg tgggacaccc gccgccgcat
    16381 ccgccggggc aagcccatcg tctgacccgt ccgttcgggc aacctgtgca ggtggcagcc
    16441 gagctggaga tggtcgaccc cgaggacgcc ggcccgctgg cggcgctgaa ggccgagacg
    16501 ttcgtggaga ccttcgccga ggacaacgac ccggaccacg tccgcgccca cgtggcccgc
    16561 gagttcaccg ccgaggcggt caggcccagc ctggcggacg atgccacgac gacctggtgg
    16621 ctgatcgacg acgacgtgcc cgtggggttc ctcaaggtga accgcggccg ctcccagacc
    16681 gaacccgacc tggacgacgg tctcgaggtc gagcagatct acgtgctggc cgcccaccac
    16741 ggccgaggtc acgggcgtcg tctgatggac cacgcactgg acacggcacg agccgagggc
    16801 ttcccgttca tctggctcgg cgtgtgggag cacaaccgca gggcgatggc gatgtacgag
    16861 cacctgggct tcgtcccgtt cggcgatcac acgttcctgt tcggcgacga ggagcagcgc
    16921 gacgtgctca tgcgtcgcga tctgtgacct cgggaggtgc ggcgaccttg tagcggtgcg
    16981 gctcggtgac gaagtcggcg tggccgtagc tgtcgagcag gtgggccagg cgagcgtcca
    17041 gctggcggtc gacgagaccg ccgacgaccg ggagccgcat gccgagccgg tagtacgcgg
    17101 tgagggcggc gatcaccccg gcggcgacgc cggctgccac cttctccctc gggccgaagt
    17161 cgatgcccat ccgttcggcc cgctcggcat ccccgccgag gaagctcttg atgagcacga
    17221 agcgggagaa cccgttctcc agccaggcgt gcgtgcgccc ggcgacgctg gacacgtcga
    17281 acagctcggc ggccatggtc ccccggacga gcgacccgca gatctcgtcg tcgtaggcct
    17341 cgcgcagcga cacgtgccga gccatcagca ggtcgacgat ctcctggggc gtctcaccca
    17401 gcagctcctc gggcaggccg aggaggaagc agcggtagcg ggacagctcg acctgggccc
    17461 gctcggcctc ggtgaactcg gtgcggccct tgcgcaggac ctgggccgac atcaagaaga
    17521 cgccgatgag gccggccggc atctggtcga cctgggggat ggggatgccg taggtggaca
    17581 cgtcccactc gccggaccgc agcaggtgga agcgcaccat cgagtgcatg agccgcacct
    17641 tggcggcggc ctcgaacccc ttgccgtgcc ggtcgagggc gccgggcatc gtcgtggcgg
    17701 tgaagaagct ggcggtctcg aacacgcgct tggccgcggc ctcgtcggac agcgtgccgg
    17761 tcatcgtcat gggcagcgcg gcgtactggt tcaggaacgt ggcgaggaac gctccccgga
    17821 tggcgaacgg ggtcgccgtg gccatcggga cccgctcggc gcgtgcgcct cgtcggacca
    17881 ggtccatgtc gacccagtcc ggcgtggcct ccatcgactc gatgagggcg acgagctcgg
    17941 gtggcgggtc atcgacggcg tcgaggccct tcgagcaggc cgtctcgatc agctcgacga
    18001 gccgccggaa gccgtgctcg gggatcagcg cggcgtaggc gtcggccaac cggtcgccgg
    18061 tcatcgtggc gtcgaggatg acgcgcatcg tgtccggctc gtcgatcacg cgctcgatcg
    18121 cgcgccgcat cgaggcgaat cgctcgtcgt cgacggtggc gtcggcgtcg tagcgctccg
    18181 ggacgatcga gaagtcgacg tcgccgtaga cctccgggat ctgctcccgc tgcctcacga
    18241 cgcggtccgc gtacaccgtc gactccatgt cccagcacct cctggcacga tgcgaccgcg
    18301 gttcgggcgc atcggctccc cgcattgtcg cggagcggcg ccgtcgccgg cccgatcagc
    18361 cggcggcgtt ggcgatcgac acccgcggat cccggttggg gtcgcccggg ttgtcgagcg
    18421 agtcgcggaa cgcggtgagc gccacgacgc cctgctcctc ggtcagctcg gcggccatga
    18481 tccgctcgac cgaggggagc aggtcaccgt gctgcgagac gatctcgtgc gccaggcgct
    18541 cccggggagc caggtcatcg acgtcggcag acccctcgga ccacttcgag ctgggcttct
    18601 tcgccttggc catggggatt cgccttcgtt cgcgggtgat cggtgacccc gacggtacgg
    18661 cggcgagtgc ccgcttcctc atttcagagg cggtcgagct tcttgaagcc ctcgccgagg
    18721 tcgcagccgc cgaagcgtcc ccactcggcg agacggccgc ggatgcgctc ggcgaacgcg
    18781 tcccactgct cgtcggcggc ggggtcgtcg agggcgacgc ccatggtggt ctggaactgg
    18841 gattcgtgcg cccgcagcgc ctcgagcttg gcctgcacgt cgcgatcctc gacggcctcg
    18901 acgtggtcgg gctcgtcggc ctcccacagc agggcgacgt cgggtcggtg gtgctcgagc
    18961 tcctgctcgg ggaagaacag cgggtcgcgg gcggcgacga tgccctcgac cgcgaggaga
    19021 ccggcgtggc ggtggtcggg gtggaggcgg tagcgcttcc acgggtcgtg gccgagcacg
    19081 accgcagggc gcaggcgccg gatccagtag gcgacctgcc agcgctggcg cagcgaggag
    19141 tcgagctcgc cgtcgggcca gccgaggaac gcgacctcgc cggtgccgcc gagcgccttg
    19201 gccgcggcgc gctgctcgac ctggcgggtg gcgaccagct cggccgggtc ctggttcggg
    19261 tcccaggtcc ccttggaccc gtcggtgcac acgaggtggt gcacgacgca gccggcggcg
    19321 gcccacttcg ccagcgtggc gccggcgccg aactcgacgt cgtcggggtg cgcagcgatg
    19381 gcgagcgcgg acgtcggcac gggcaggtcg cggctgacgc cggtggacgc gccgggcggg
    19441 gagggcgtgg gcaggtcggg ggtgctcatc ggctcagaag tgcgccggca gcgcgaggtc
    19501 ggcggggtgg tcgacgtccc agccgaggcg ggcgtcgcgg acgatgcgca ccggcagccc
    19561 cagccgcagg ccctcggccc ggtggcgatc gaaggaggcg gcgccgtagg cgaaccgga
//
LOCUS       DATISU010000209         3718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_56669, whole genome shotgun sequence.
ACCESSION   DATISU010000209 DATISU010000000
VERSION     DATISU010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56669"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VLR27_08755"
     CDS             <1..395
                     /locus_tag="VLR27_08755"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HSP03575.1"
                     /translation="QQATATVLEWPAGQWIVTAVGLGIIGYAIYMFKKHVMDEEFLQR
                     LSTSKDWVEKLGRGGYAARSAIFLVIGWFLTQAGLTHEAGETKGLSGALQEISGQGWG
                     QLLLLAVAVGLLAFGLFSLAEAKSRRDA"
     gene            515..736
                     /locus_tag="VLR27_08760"
     CDS             515..736
                     /locus_tag="VLR27_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HSP03576.1"
                     /translation="MSTNETTTSTITVAGMTCGHCVSSVKEQIGKIDGVESVDVDLAS
                     GVVTVESATPTPDAALEAAIADAGYEVAT"
     gene            733..1620
                     /locus_tag="VLR27_08765"
     CDS             733..1620
                     /locus_tag="VLR27_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HSP03577.1"
                     /translation="MRPAVVLGAFGVVLAAAFGGGATAGSLAGPIDTAGGGDHGGDPA
                     DEAPIELPAGGLLVAQDGLHLEPADRLADAGPFRFRIVDAGGSAVTDFVEQHEREMHL
                     IVAARDLQTFHHIHPHMDAAGDWQVDLPALPAGSYRAFADFQADGFVPMTLGVDLVAP
                     GSAEAPRPLTTSRTDATDGYDVDIDGTLDAGGSSELTITVQRDAEVVTTEPYLGAGGH
                     LVALRDGDLAFLHVHPLADDTTGPVRFAVEVPSAGTYALFFDFAHAGEVHTARFVLDA
                     RSVEPGAPSDTPDQHDEGH"
     gene            1625..>3718
                     /locus_tag="VLR27_08770"
     CDS             1625..>3718
                     /locus_tag="VLR27_08770"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HSP03578.1"
                     /translation="MTTTSTTDPAARPDAEATSIDLSIGGMTCASCAMRIEKRLNKLD
                     GVTATVNYATEKAKVHAPPGVSITELIAQVEAAGYTASTPTLAPDPADRPGEEPPDET
                     AALRARLLVSAALALPVVVLAMIPAAQFDGWQWLSLTLAAPVVTWAALPFHRAAWVNA
                     RHGTATMDTLISVGVLAAFTWSLYALFLGTAGEIGMTHGFSWTAERGAGDSQVYLEVA
                     AGVTVFILAGRYFEARAKRRSGAALRALLELGAKDVAVLRDGTEVRIPIGELRVDDRF
                     VVRPGEKVATDGIVEDGASAIDASMLTGESVPVEVCTGDPVAGATVNAGGRLVVRATR
                     VGSDTALAQIARLVEDAQTGKAAVQRLADRVSAVFVPVVITLAFATLGFWLTAATTDS
                     ASAAFTAAVAVLIIACPCALGLATPTALMVGTGRGAQLGILIKGPEVLESTRRIDTIV
                     LDKTGTVTTGRMALAEVHTADGTTEAEVLRLAGSLEDASEHPIARAIAEGARTRGVEL
                     TDVESFASTDGLGVQGVVDGHAVVAGRERFLLDWSLHLPEDLRAVKDTAEAAGRTPVL
                     VAWDGAVRAVVVVSDTVKATSADAVRQLHDLGLRTVLLTGDNARAARAVADQVGIDEV
                     IAEVLPEDKLAVVRRLQDEGRVVAVAGDGVNDAAALAQADLGLSMGTGTDVAIEASDI
                     TLVRGDLRSAADAIRL"
BASE COUNT          516 a         1453 c         1232 g          517 t
ORIGIN      
        1 accaacaggc gacggcgacg gtgctcgagt ggccggccgg tcagtggatc gtgacggctg
       61 tcggactcgg gatcatcggg tacgcgatct acatgttcaa gaagcacgtc atggacgagg
      121 agttcctcca gcggctctcg acgagcaagg actgggtcga gaagctcggc cggggtggct
      181 acgccgcccg gtcggcgatc ttcctcgtca tcggctggtt cctcacgcag gccggcctga
      241 cccacgaggc cggggagacc aaggggctct ccggtgcgct gcaggagatc tccggccagg
      301 gctggggtca gctcctgctg ctcgccgtgg ccgtcggcct gctggccttc ggcctcttca
      361 gcctggccga ggccaagtcc cgacgcgacg cctgagcggc accgcgctac gggctgggca
      421 acaccaacgg cttgacgtcg cgcgttcttg ttacttgaca ggtcccccta gggggtacga
      481 gtatgagtct cctcaacccc gagaggagct ccccatgagc acgaacgaga ccaccacctc
      541 caccatcacc gtcgccggca tgacctgcgg gcactgcgtc agctcggtga aggagcagat
      601 cggcaagatc gacggcgtcg agtccgtcga cgtcgacctc gcatccggcg tcgtgaccgt
      661 cgagtcggcg acgccgaccc cggacgcagc gctcgaggcg gccatcgccg acgcgggcta
      721 cgaggtggcg acgtgaggcc cgccgtggtc ctcggcgcct tcggcgtggt gctcgccgcc
      781 gccttcgggg gcggcgcgac cgccggctcc ctggccgggc cgatcgacac cgccggtggc
      841 ggcgaccacg gcggcgaccc cgccgacgag gcacccatcg agctgcccgc cggcggcctc
      901 ctcgtcgccc aggacggtct gcacctcgag ccggcggacc gcctcgctga cgcgggcccg
      961 ttccgcttcc gcatcgtcga tgcgggcggc tcggcggtca cggacttcgt ggagcagcac
     1021 gagcgcgaga tgcacctgat cgtggccgct cgggacctgc agacgttcca ccacatccac
     1081 ccccacatgg acgctgccgg cgactggcag gtcgacctcc cggccctgcc cgccgggtcg
     1141 taccgggcgt tcgccgactt ccaggccgat gggttcgttc cgatgaccct cggcgtggac
     1201 ctcgtcgctc cgggatccgc tgaggcgccc cgaccgctca ccaccagccg caccgatgcc
     1261 accgacggct acgacgtcga catcgacggc accctcgacg ccggagggtc gtcggagctg
     1321 acgatcaccg tgcagcgcga cgctgaggtc gtcaccaccg agccgtacct cggcgccggc
     1381 ggccacctgg tcgcactgcg agacggcgac ctggcgttcc tccacgtgca ccccctcgcc
     1441 gacgacacca ccggtccggt gcgcttcgcc gtcgaggtgc cctcggcagg cacgtacgcg
     1501 ctgttcttcg acttcgccca cgccggagag gtccacaccg cccgcttcgt cctcgacgcc
     1561 cgcagcgtcg agcccggcgc gccatccgac acgcccgatc aacacgacga aggccactga
     1621 tcccatgacc accacctcca ccaccgaccc ggccgctcgg cccgatgcgg aagccacatc
     1681 gatcgacctg tccatcggcg gcatgacgtg cgcatcgtgc gccatgcgca tcgagaagcg
     1741 cctgaacaag ctcgacggcg tgacggccac cgtcaactac gccaccgaga aggccaaggt
     1801 ccacgcgccc cccggcgtgt ccatcaccga gctgatcgcc caggtcgaag cagctggcta
     1861 cacggcctcc accccgacgc tggcgccgga ccccgccgac cggcccggtg aggagccgcc
     1921 cgacgagacc gccgcgctcc gcgcccggct gctcgtgtcg gctgccctcg ccctgccggt
     1981 cgtggtcttg gccatgatcc ccgcggccca gttcgacggt tggcagtggt tgtcgctgac
     2041 cctggctgca ccggtcgtca cctgggcagc gctcccgttc caccgcgccg cgtgggtgaa
     2101 cgcccgtcac ggcaccgcga cgatggacac gctcatctcc gtcggcgtgc tcgccgcgtt
     2161 cacctggtcg ctgtacgccc tcttcctcgg caccgccggc gagatcggca tgacccacgg
     2221 gttctcctgg accgccgagc gcggcgccgg cgactcccag gtgtacctcg aggtcgccgc
     2281 aggcgtgacc gtcttcatcc tcgccggccg ctacttcgag gcccgggcca agcgacgttc
     2341 cggtgccgcg ctccgagcgc tgctcgagct cggcgccaag gacgtcgccg tgctccgcga
     2401 cgggaccgag gtccgcatcc cgatcggcga gctccgggtc gatgaccgct tcgtcgtccg
     2461 ccccggggag aaggtggcca ccgacgggat cgtcgaggac ggcgcctcgg ccatcgacgc
     2521 gtcgatgctg accggcgaga gcgtccccgt cgaggtctgc accggcgatc ccgtggccgg
     2581 cgccaccgtc aacgcgggag gccgcctcgt cgtgcgcgcc acccgggtcg gctccgacac
     2641 cgcgctcgcc cagatcgccc gactcgtgga ggacgcccag accggcaagg ccgccgtcca
     2701 gcgcctcgcc gaccgggtct cggcggtgtt cgtgcccgtc gtcatcaccc tggccttcgc
     2761 cacgctcggg ttctggctca ccgccgcgac caccgacagc gcctcggcag cgttcaccgc
     2821 cgccgtggcc gtcctgatca tcgcctgccc ctgcgccctc gggctcgcca cacccacggc
     2881 gctcatggtc ggcaccggac gcggcgccca gctcggcatc ctcatcaagg gccccgaggt
     2941 cctcgagtcc acccggcgca tcgacacgat cgtcctcgac aagaccggaa ccgtcactac
     3001 cggacgcatg gcgctcgccg aggtccacac ggccgacggc accaccgagg ccgaggtcct
     3061 ccgcctggcc ggctcgctcg aggacgccag cgagcacccc atcgcccggg cgatcgccga
     3121 gggtgctcgg acccgagggg tggagctcac cgacgtcgag tccttcgcca gcaccgacgg
     3181 gctcggcgtg cagggcgtcg tcgacggtca cgccgtcgtc gccggccggg agcggttcct
     3241 cctcgactgg tccctgcacc tgcccgagga cctgcgcgcc gtgaaggaca ccgcggaggc
     3301 ggctgggcgc acgcccgtgc tcgtcgcgtg ggacggcgca gtccgtgccg tggtggtggt
     3361 gtccgacacc gtgaaggcca cctcggccga cgcggtccgc cagctccacg acctcggcct
     3421 ccgcaccgtg ctgctcaccg gcgacaacgc ccgagcggcc cgtgccgtgg ccgaccaggt
     3481 cggcatcgac gaggtcatcg ccgaggtgct cccggaggac aagctcgccg tcgtccggcg
     3541 cctccaggac gaaggccgtg tggtcgccgt cgccggcgac ggggtcaacg acgccgccgc
     3601 gctggcccag gccgacctcg ggctgtcgat ggggaccggc accgacgtcg ccatcgaggc
     3661 cagcgacatc accctcgtgc gcggcgacct ccgatccgcc gccgacgcga tccggctc
//
LOCUS       DATISU010000210         4046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_615689, whole genome shotgun sequence.
ACCESSION   DATISU010000210 DATISU010000000
VERSION     DATISU010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615689"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /gene="ispG"
                     /locus_tag="VLR27_08775"
                     /gene_synonym="gcpE"
     CDS             <1..1206
                     /gene="ispG"
                     /locus_tag="VLR27_08775"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HSP03579.1"
                     /translation="PRRTTRQIQVGDVAVGGGAPISIQSMTVTKTADVDATLQQIYAL
                     AAAGCDIVRCTCNEPEAAEGLARIVPRSPVPIVADIHHQYRRALEALEAGVHCLRLNP
                     GNIRKPEHNKAVAMEAKDRGVPIRIGVNGGSLDPALYEKYGGKVTPEAMVESAKIEIG
                     YFEEVGFEDIKISVKASSVPLMIEAYRMLADEVDFPLHLGVTEAGPPPNGLIKATAGI
                     ATLLAEGIGDTIRYSLTADPVQEAKAGRQLLESLGLRERKNVDLIACPSCGRAEIDVI
                     AVAEQAMAAFGEREIPLQVAVMGCVVNGPGEARDADIGIAAGNKRGHLFVRGTNVAVV
                     PEDEMVGALVEWAEFIHEHGIDKAMEKADLTAAKEAAEADRAMLLEEQGDDANASEQV
                     VELIRKGRG"
     gene            complement(1203..3134)
                     /gene="recD"
                     /locus_tag="VLR27_08780"
     CDS             complement(1203..3134)
                     /gene="recD"
                     /locus_tag="VLR27_08780"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HSP03580.1"
                     /translation="MTVLLGRQAHRASGVLGAFATAGVLEAADVHVAQTLDRLSGAPS
                     DADTELTMLAIALTVRAVRHGSVITDLATLAGTVTVDEVPLGPDGAPVVDVADLPWPE
                     PDRWLAAVRASPLVADGDDAPTSRPLRLVGTALALDRYWRHERRIVADLTTRAATTPT
                     GVDDDLLRAGLGRLFPGSPSAGPDLQRVAAAAAVLRSCTVIAGGPGTGTTTTVARILA
                     LLHEQAAADGRPMRAALAAPTGKAAARLQEAVQEQAARLAADGAVAPAIADAVAGTEA
                     ATLHRLLGWNPGNRSRFRHDRANQLPHQVVVVDETSMVSLSMMARLLEAVRPSARLVL
                     VGDPQQLASVEAGAVLGDVVGPAAERLRMRPAARTELEEVSGQVVDADEDDRPVCIGD
                     GIVVLRHNHRFAGGIRDLAAAIQTGRADEVVDVLGRDTHDVHWLPVDVAEEPVPAIRD
                     LVVDQAAEVIEAATHGRAGDALAAMRGVQLLCAHRRGPYGVATWRREIERRLAETVPG
                     FAWQGWFAGRPLLITENDRQLGLYNGDTGVVVDDAGTLRCAFERRGEVVHVSPSRLAA
                     VETLYAMTIHKSQGSQFGEVIVVLPDESSPILTRELLYTAVTRAERGVTVVGTEAAVR
                     AAVSRPVARASGLRDRLWG"
     gene            complement(3131..>4046)
                     /locus_tag="VLR27_08785"
     CDS             complement(3131..>4046)
                     /locus_tag="VLR27_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HSP03581.1"
                     /translation="ERGLEDEQMPEELLAGGDPTDAETEARLRAVPSPFGDLGGGATF
                     GTLVHGVLEEVDFAADDLPGQIRAGLAAQYLGSQQVDADALVAGLVAAIETPLGPLVD
                     EARLRDVQPADRLDELHFELPLVGGDDPTGHVTMRAIADVVSRHLPADDPLAGYGERL
                     RSPALAAEVRGHLSGSIDVVLRHRGSDGTGRYVVIDHKSNWLGVAGEELSAWHYRPSA
                     LADAMVRAHYPLQALLYSVALHRYLRWRLPDHDPERDLGGVLYLFLRGMTGADVPRVD
                     GQPCGVFSWRPPAAMVVGLSDVLDRGTS"
BASE COUNT          555 a         1451 c         1559 g          481 t
ORIGIN      
        1 ccccgacgca ccacccgcca gatccaggtc ggcgatgtcg ccgtcggcgg cggtgcgccc
       61 atcagcatcc agtcgatgac ggtgaccaag accgccgacg tcgacgccac cctccagcag
      121 atctacgccc tcgccgccgc cggctgcgac atcgtgcggt gcacgtgcaa cgagccggag
      181 gcggccgagg ggctggcccg catcgtgccc cgctcgccgg tgcccatcgt cgccgacatc
      241 caccaccagt accgccgggc cctcgaggcg ctcgaggccg gcgtccactg cctgcgcctc
      301 aacccgggca acatccgcaa gcccgagcac aacaaggccg tggcgatgga ggccaaggac
      361 cgaggcgtgc ccatccgcat cggcgtgaac ggcggcagcc tcgaccccgc gctgtacgag
      421 aagtacggcg gcaaggtcac cccggaagcc atggtcgagt cggccaagat cgagatcggg
      481 tacttcgagg aggtcggctt cgaggacatc aagatctcgg tgaaggcgtc gtcggtgccg
      541 ctgatgatcg aggcgtaccg gatgctggcc gacgaggtcg acttcccgct gcacctcggg
      601 gtcaccgagg ccggcccgcc ccccaacggc ctcatcaagg ccaccgccgg catcgccacg
      661 ctgctggccg agggcatcgg cgacaccatc cggtactcgc tcaccgctga cccggtgcag
      721 gaggccaagg ccggccgcca gctgctcgag tccctcgggc tgcgggagcg caagaacgtc
      781 gacctcatcg cttgccccag ctgcggccgg gccgagatcg acgtcatcgc cgtcgccgag
      841 caggccatgg ccgcgttcgg cgagcgcgag atcccgctgc aggtcgcggt catgggctgc
      901 gtcgtcaacg gacccggcga ggcgcgcgac gccgacatcg gcatcgccgc gggcaacaag
      961 cgcggccacc tcttcgtgcg gggcaccaac gtggccgtcg tgcccgagga tgagatggtc
     1021 ggcgccctgg tcgagtgggc cgagttcatc cacgagcacg gcatcgacaa ggcgatggag
     1081 aaggccgacc tcaccgccgc caaggaggcc gccgaggccg accgggccat gctgctcgag
     1141 gagcagggcg acgacgccaa tgcctccgag caggtcgtcg agctgatccg caagggtcgg
     1201 ggctaacccc agagacggtc ccgcagtccc gatgcccggg cgacggggcg ggagaccgca
     1261 gcacggacgg cggcctcggt gccgacgacc gtgacgccgc gctcggccct cgtcacggcc
     1321 gtgtagagca gctcccgggt caggatcggc gacgactcgt cgggcagcac gacgatgacc
     1381 tcgccgaact ggctgccctg gctcttgtgg atcgtcatgg cgtagagggt ctcgacggcg
     1441 gcgagccggc tgggtgagac gtgcacgacc tcgccccggc gctcgaacgc gcagcgcagg
     1501 gtgccggcgt cgtcgacgac gacgccggtg tcgccgttgt agaggccgag ctggcggtcg
     1561 ttctcggtga tgagcagggg ccgaccggcg aaccagccct gccaggcgaa gccgggcacg
     1621 gtctcggcca gccggcgctc gatctcacgc cgccacgtcg ccaccccgta gggaccacgt
     1681 cggtgggcgc acagcagctg cacgccccgc atcgcggcca gggcgtcgcc ggcccggccg
     1741 tgggtggcgg cctcgatgac ctcggcggcc tggtcgacca cgaggtcccg gatggcgggg
     1801 acgggctcct cggcgacgtc gacggggagc cagtgcacgt cgtgggtgtc gcgaccgagc
     1861 acgtcgacga cctcgtcggc gcggccggtc tggatcgcgg cggcgaggtc gcggatgccg
     1921 ccggcgaagc ggtggttgtg gcggagcacg acgatgccgt cgccgatgca caccggccgg
     1981 tcgtcctcgt cggcgtccac cacctggccg ctcacctcct ccagctccgt gcgggcggcc
     2041 ggtcgcatgc ggaggcgctc ggccgcgggt ccgacgacgt cgccgaggac ggcgccggcc
     2101 tccaccgacg ccagctgctg ggggtcgccg accagcacca ggcgggctga ggggcggacc
     2161 gcctcgagca gccgggccat catcgacagc gagaccatcg acgtctcgtc cacgaccacg
     2221 acctggtggg gcagctggtt ggcgcggtcg tggcggaagc ggctccggtt ccccgggttc
     2281 cagcccagca gccggtggag ggtggccgcc tcggtgccgg cgacggcgtc ggcgatcgcc
     2341 ggtgcgaccg ccccgtcggc ggcgaggcgg gccgcctgct cctgcacggc ctcctgcagc
     2401 cgggccgccg ccttgccggt gggcgcggcg agcgcagccc gcatcggccg gccgtcggcc
     2461 gcggcctgct cgtggaggag ggcgaggatc cgggcgaccg tggtcgtcgt gccggtgccg
     2521 ggcccgccgg cgatgacggt gcaggaccgc agcacggcgg ccgcggccgc gacccgctgg
     2581 aggtccggac cggccgacgg ggaccccggg aagaggcggc cgagcccggc gcgcaggagg
     2641 tcgtcgtcca cgcccgtcgg ggtggtggcg gcccgggtgg tgaggtcggc cacgatccgg
     2701 cgctcgtggc gccagtagcg gtcgagggcc agggcggtgc cgaccaggcg cagcggtcgg
     2761 gaggtggggg cgtcgtcgcc gtcggcgacc agcggggagg cgcgcaccgc ggccagccag
     2821 cggtcgggct cgggccaggg caggtcggcc acgtccacca cgggggcgcc gtcggggccg
     2881 aggggcacct cgtccaccgt cacggtgccg gccagggtgg cgaggtcggt gatgacgctg
     2941 ccgtggcgca cggcgcggac cgtcagcgcg atggccagca tcgtgagctc ggtgtcggcg
     3001 tcggacggtg cacccgacag tcgatccagg gtctgggcca cgtggacgtc ggccgcctcg
     3061 agcacgccgg ccgtcgcgaa cgcaccgagc acgccgctgg cccggtgggc ctggcggccc
     3121 aggagcacgg tcatgacgtc ccccgatcga gcacgtcgga caggcccacg accatcgcgg
     3181 cgggcggtcg ccaggagaag acgccgcacg gctgaccgtc gacgcgcggg acgtccgcac
     3241 cggtcatgcc gcgcaggaac aggtagagga cgccaccgag gtcgcgctcg gggtcgtggt
     3301 cgggtaggcg ccagcgcagg taccggtgca gggccacgct gtagagcagg gcctgcagcg
     3361 ggtagtgggc ccggaccatg gcgtcggcca gggcgctggg gcggtagtgc cacgccgaca
     3421 gctcctcgcc ggccacgccc agccagttgc tcttgtggtc gatgacgacg tagcggccgg
     3481 tgccgtcgct tccgcggtgg cgcagcacca cgtcgatgga tccgctgaga tggccccgca
     3541 cctcggcggc cagcgccggc gatcgcagcc gctccccgta cccggccagc gggtcgtcgg
     3601 ccggcaggtg gcgggagacc acgtcggcga tggcccgcat ggtgacgtgg ccggtggggt
     3661 cgtcgccgcc gaccaggggc agctcgaagt gcagctcgtc gaggcggtcg gccggctgca
     3721 cgtcccggag gcgggcctcg tccaccaggg ggccgagcgg cgtctcgatg gcggcgacca
     3781 ggccggcaac gagcgcgtcg gcgtccacct gctggctgcc gaggtactgg gcggcgaggc
     3841 cggcgcggat ctggccgggc aggtcgtcgg ccgcgaagtc gacctcctcg agcacgccgt
     3901 gcacgagcgt gccgaacgtg gcgccgccgc cgaggtcgcc gaacggggag ggcacggcgc
     3961 gcagccgggc ctcggtctcc gcgtcggtcg gatccccgcc ggcgagcagc tcctcgggca
     4021 tctgctcgtc ctcgaggccc cgctcg
//
LOCUS       DATISU010000211        15445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_708717, whole genome shotgun sequence.
ACCESSION   DATISU010000211 DATISU010000000
VERSION     DATISU010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 15445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15445
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708717"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VLR27_08790"
     CDS             <1..449
                     /locus_tag="VLR27_08790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HSP03582.1"
                     /translation="GAVWLVATHVGYSVISWVFPAFGDQVRDLYRLGDEDTASRMVGP
                     IAAMGVAEELLFRGVLQGVGGIALALVAYTAVQVFERKWALALAALLGGVVWGALFAW
                     RGGLVAPIAAHVLWTGMLTFVWTLGTAPRVQESGVTPATRQRDHAS"
     gene            complement(463..2088)
                     /locus_tag="VLR27_08795"
     CDS             complement(463..2088)
                     /locus_tag="VLR27_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP03583.1"
                     /translation="MASLFDRVSVNLQAVDTIRRMGLLKPKVAASALQSAKRWGPTLA
                     AGYAAGVARRGDEVAIVDDDGTLTFREVHEQSSALANALARRGVGPETPVALLARNSR
                     WFVLAVAALDKLGATVLYMNTGFAGPQLAEVLEREGCDLLLHDDEFSDAVAGYAPDVQ
                     RLVCDRAPADGTDDLPTLVAATSTQDPDKPERQGRQVILTSGTTGTPKGASRSKESLG
                     AGLVAATAIFQRVPFRTEDVHAVPAPMFHSLGNASLLAAATMSHTLVLSRRFDPEHLL
                     AQIEANRVRVVTAVPVMLQRIVALPEAVTERYDTSSLEVVFCSGSALPGSLATQWMDR
                     FGDNLYNMYGSTEVAVATIATPEDLRAAPGTAGKPLAGVDVRLYDDDDRRITGPDTVG
                     RIFVGSALRFEGYTGGQTKPAIDGLLSSGDLGRFDTDGRLFVGGRDDDMIVSGGENLF
                     PSEVEDLLSDMEGVREVAVLGVDDDQFGQRLAAFVVTEDGVDLDDRAVKDHVKANLAN
                     YKVPRSVWFVDDLPRNPTGKVLKRELRERFPDA"
     gene            complement(2110..3582)
                     /locus_tag="VLR27_08800"
     CDS             complement(2110..3582)
                     /locus_tag="VLR27_08800"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HSP03584.1"
                     /translation="MSEQTPSREPDVEHDQTPPDQTAPDTPPAGQADFVVVANRLPVR
                     LEDDGSWALSPGGLVSAMLPVMRDRTGSWVGWDGSDDGEAQRFDFEGITLSALALSHQ
                     EREEYYGGACNETIWPLFHDAIETTQFHRETWAAYRTVNQRFADAACAELAEGGTVWV
                     HDYQLQLVPRMIREQRPDVRIGFYLHIPFPPLELFSRLPWRAELLAGLLGADVIGVQT
                     QADARNVLYGVRRFCEVDDLTFAGDGGVVRTGRDTSVVKAFPISVDYEAIAEQAASDE
                     VELAVKELRKELGDPKHVLLGVDRLDYTKGIDLRLKAIHELLIEHRLDPRSTAVIQVA
                     VPSREDVQGYDSVRNRVEQLVGTINGDYGTLGRPVVQYIHKSLPFAELLVLYRAADVL
                     LVTPFQDGMNLVAKEYVSARVDDTGALVLSEFAGAAEELNAAFLVNPYDTEHLKEQIA
                     AAVSMDEDEQRAHMRVMRDTVQRNDLAAWATAFLALLSIS"
     gene            3735..4199
                     /locus_tag="VLR27_08805"
     CDS             3735..4199
                     /locus_tag="VLR27_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03585.1"
                     /translation="MGIIRKTLSVTTLGLVGWKSKKQLLAEAEADLASTRADLEQTSA
                     LRSALQERLQNVEHDLESSELDALKGAKVARKDERRRMRREKAEAQAARARKQAEARA
                     EEARARLQKTSKSARKAAERARKKAEHLADDARHQVDDVTKTVRKKRKKLRG"
     gene            complement(4428..4877)
                     /locus_tag="VLR27_08810"
     CDS             complement(4428..4877)
                     /locus_tag="VLR27_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSP03586.1"
                     /translation="MKVLIAVDDEPESEDAMSFAQQLVPDDAAVIVVNVARSTITPYG
                     TIGGLGAAYAGSPTLSAEADVRAQEMADREAEQVVERAGEALHADERRVEHGDVGPTI
                     CKVAEDEGVDLVVLGTHDRSRWSRLWFGSVSDHVVRHAPCPVLVVRR"
     gene            complement(4914..6191)
                     /locus_tag="VLR27_08815"
     CDS             complement(4914..6191)
                     /locus_tag="VLR27_08815"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSP03587.1"
                     /translation="MRLLPTSRPIRIGLVAALVLVVILGVAFRQQLGDVYGYVFPDGI
                     VDDAEDRTSAPAPTTQFEPTTTNTTEATSTTTEPPLETGTDPAIALGPVVDLPGPSAA
                     VDPDGPGPILVSTLDGRVHAVDVDGGTSEVVLDLSDRVSTGGERGLLGLALDPSGDRL
                     YLNYTNSAGDTEVRSVAMDGDRPAEGVEASVLHLEIGQPYRNHNGGNLVFGPDGALWI
                     GVGDGGSANDPAGVAQDPDVLLGKMLRVVPDPAGGVSAPASNPEWGERPEVWAIGLRN
                     PWRYSFDRATGQLWVADVGQNAREEVTVVDPGADDRVNFGWNRLEGTRPLAGEPGPEL
                     TDPQLEYTHDEGCSITGGYVYRGEAVPSLYGWYLFADFCGGWVRAVPAEDPGAQPVEL
                     VSGFGPVLSFAELEDGELLVLGQDGIHAVDAPG"
     gene            complement(6310..6897)
                     /locus_tag="VLR27_08820"
     CDS             complement(6310..6897)
                     /locus_tag="VLR27_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03588.1"
                     /translation="MTRRRIDHLLIGAGVVAAIVLAIAGGLLTWGSNFAEDYVGDELA
                     AQNISFPPTEALIEDGREDLAEYGGQQVDTGEEAEAYASFIQGHVEATAGGLTYAEIP
                     DREARTALQEAIDSGAPEAEIAELEQEATTLSQQRDTVFKGEMLRGTLLNAYAWSTMG
                     QIAGIAATAAFAAAAVMLVLSVAGGVHLRKVSRTS"
     gene            complement(7119..8078)
                     /locus_tag="VLR27_08825"
     CDS             complement(7119..8078)
                     /locus_tag="VLR27_08825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HSP03589.1"
                     /translation="MTTYGYFLSSEEHTPTELVRQAVLAEEAGFEALWISDHFHPWND
                     AQGESPFVWGVIGAIANATSLPITTAVTCPTIRIHPAIIAQAAATSALLTDGRFNLGV
                     GTGENLNEHVLGDRWPPADVRLEMLEEAIEVMRALWTGDQVSHHGRHYTVENARLYSN
                     PDTPPPVYVSGFGPKAVSLAARVADGYCGTSPDAELVEQYRSEGGSGPAQAGVKVCWG
                     SDRDQCVKTAHRLWGPNGVPGEASQELPMPAHFEQAGALVTEEMVSEKFACGPDLDEH
                     LASIREFDEAGYDEIFVSQIGETSEGFFRFAREDLLPALRDGG"
     gene            complement(8135..9079)
                     /locus_tag="VLR27_08830"
     CDS             complement(8135..9079)
                     /locus_tag="VLR27_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSP03590.1"
                     /translation="MVSVALVLGGGGIAGAAWHGAVLAALDEVGWDARRADLVIGTSA
                     GSNVAAVLRLGVPPSDLVAGALGRPLSPQGAAHAARAGGPMDLPAPKLGWARPVAPLL
                     ALRALARPWRANPMLAVAGLLPTGRVPTAVLGDRIRATHDDRWPDRTTWICAVRMPEG
                     RRTVFGRDVDDADLATAVEASSAIPGYFAPVEHGGATYVDGGVHSPTNADLAAELGFD
                     IVVISSPMSGTRAALRTGWTGARPLHRATLAREVRALRRGGARVLVLQPGPEVVAAVG
                     PSSMDPARRRHITEVAHASVRAHLASPLVAERLDLLRS"
     gene            9116..10657
                     /locus_tag="VLR27_08835"
     CDS             9116..10657
                     /locus_tag="VLR27_08835"
                     /inference="COORDINATES: protein motif:HMM:NF018530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HSP03591.1"
                     /translation="MDLISEHPVLLLFLVIGVGSAVGRLRVRGVGLGPAAVLFTGLAA
                     SGIDPDLVLSEEVMELGLVLFTYTVGLATGPAFVAGLRGRGLRSLAVVAIATSAAAVV
                     AAAAGVVLDLGSVEVAGLFAGATTNTPALGAVLDAAGPEGPGAAITYAIAYPAGVLVA
                     LLAASAALRTGPAPEQDDDIVAWAVLVTRDRVALGQLPSGVSFGRYIRSGASEVATPD
                     TVVVEGDVVTVVGPGGEVAAVAAALGDHVQKGGVVLDRSTVDFRRITVSDPAVIGRRL
                     GELDLSGRFGATATRVRRSDVDMVARPDMVLEAGDRVRVVAPVAELHAVSALFGDSET
                     QLTDIDFVALSAGLVVGVLFGLVTFPGGLRLGAGGGALLAGLALGAAGRLGPVSFSIG
                     HQVSTGLRHLGTVVFLAAVGTRSGEAFAASAFTVDGAEVAAASVAVVAAAVLALRWTV
                     GRLAHLEGAAASGAVAGLQTQPAVLAFADSSSGHSPQVPLGYTALYPAAMVAKIVLAQ
                     LLVRL"
     gene            10704..11618
                     /locus_tag="VLR27_08840"
     CDS             10704..11618
                     /locus_tag="VLR27_08840"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HSP03592.1"
                     /translation="MIFATDAANNAARRFEHIEVRPLAGALGAEVFGPGGAPLDVTAL
                     SEAAVAEVRQASVDHLVVFFRDQSLDLDSFSAFASTWGPYGDDPFVEGLESHPHVVKV
                     VKEADEKVPLVFGGAWHSDWSFQAEPPAFTILYGHDVPDHGGDTLWANLYLAAQHLSP
                     GLRATLADLRAVHSPQKAYGAQATHNDLVEHMAIRYGEDAHLRRAQPILRQHPDSGRT
                     ALFVNWGYTESIEGWWPDESRALLDHLFEVSQHPAYTCRFRWEPGSIAVWDNRCTLHN
                     PIADYAGQRREMWRITVRGEEPIPAGHP"
     gene            11629..12441
                     /locus_tag="VLR27_08845"
     CDS             11629..12441
                     /locus_tag="VLR27_08845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSP03593.1"
                     /translation="MPIPFPAPDRTCIVTGASSGIGTEIARVLASRGHGVTLVARRLD
                     RLETLAGQLSERHGIRAEVVVADLTEPAQRRAVIEAVDERGLTPEVLVNNAGFSTVGP
                     VVDVDPGREIQMLRTNVEAVDHLSALVAPGMVERGRGAILNVASTASYQPLPGQAGYA
                     ASKAFVRSFTTALAGELAPHGVTVTALCPGPVRTEFGHASGMDDFTDGNPLPEAMWVP
                     VEHVGRIAVDALEDGRLVCIPGPLNRLVAAVANVTPRQVLLPVLVQRHPSLR"
     gene            12438..13742
                     /locus_tag="VLR27_08850"
     CDS             12438..13742
                     /locus_tag="VLR27_08850"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSP03594.1"
                     /translation="MTSPHRRGQADLDLLPVGVAHVDPAGTIVRANRMIGELVGVPAD
                     TVVGRSLFEFAAGDMDRYAAMLDFSTGYDALMGPVAVTYRRADGSLRQSALWARNHLD
                     DPDVAALVCTFVPEDTTGGLAAALASVAEGDDVDDTLALLSAALRGNPFEARGCWLVR
                     DGSGRRLAGDADLIDAARAALAQPGAWWRATRGTGLVTCDDTRHGDEYDRLLSAAGIV
                     AWWALPCQGSDDAEAAILVLRTELGPLSPLQHEQLDQLATTAGLAFQRAAMQARLAHA
                     AFHDPLTGLANRGRFFHRDDARLQAGAALLYVDLDRFKPVNDRLGHTAGDLVLVTVAD
                     RIRRAVRPSDRITRFGGDEFVVECAGVATDDEAVVIAERIIAAIQEPIRLDLGEIQIG
                     ASIGIARTDEAVGVDDLLDRSDVALLAAKAAGKGRWHLAGPG"
     gene            complement(13743..14564)
                     /locus_tag="VLR27_08855"
     CDS             complement(13743..14564)
                     /locus_tag="VLR27_08855"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106411 - XMP 5'-nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HSP03595.1"
                     /translation="MRILVTNDDGIDSLGLHVLARRLTELGDVVVVAPDREYSGFGAA
                     LGTLHLMQPEVQREHIDGVPEAWSVSGPPALCVYFARFGCFGDIDVVVSGINPGANTG
                     RAIYHSGTVGAAITARNGGLNGIAVSQSVIGFGVEGQGWDEMIQDQRWDTAAEVAARV
                     TGSLIDDLPDPPLVVNINVPNVGLEEITAWRHAEVGTIPPRSMSSATLVPREGYEETF
                     TVEMEWGDPVELPIETDGGSVEAGEVSISYLSRIVHEPGATVAAVDAVLDELTGG"
     gene            complement(14627..>15445)
                     /locus_tag="VLR27_08860"
     CDS             complement(14627..>15445)
                     /locus_tag="VLR27_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03596.1"
                     /translation="RTTAARAPASTEPLRIPARLALALCLVLAACGGDDVPVERAGGA
                     APLPADSAVEAPPDPTPTAVPTTLAPPTSAAPPTTAAPPTTAAIPRVGVDTGFAPFAT
                     VGEVVLAHPAAQVETVGFHESNHDGARQMDPTAEAARPLELESRERGTGSRSAADIVV
                     SPDAEIRSPVTGTVKRAGGYILYCDHEDDYAVIEPDARPGWEVKVLHIDGVQVQAGQR
                     VEAGATVLAPRPTLLPFASQVDDHTASPAWPHVHIEVVDISIPDRPSPGGGGGC"
BASE COUNT         1991 a         5613 c         5673 g         2168 t
ORIGIN      
        1 caggcgcggt gtggctggtc gccacccacg tcggctactc cgtcatcagc tgggtgttcc
       61 ctgccttcgg cgaccaggtc cgcgacctct accgcctcgg cgacgaggac accgcgtccc
      121 gcatggtcgg ccccatcgcc gcaatgggcg tcgccgaaga gctgctgttc cgcggcgtcc
      181 tgcagggcgt cggcggcatc gccctcgcgc tcgtcgcgta cacggccgtg caggtcttcg
      241 agcggaagtg ggccctggcc ctggccgccc tgctcggcgg cgtggtgtgg ggggcactgt
      301 tcgcctggcg cggcggcctg gtggccccca tcgccgccca cgtgctgtgg accggcatgc
      361 tcacgttcgt gtggaccctc ggcaccgcac cccgcgtgca ggagtcgggc gtcacgccgg
      421 ccacccgcca gcgcgaccac gcgtcctgac tgggcgtctt gactacgcgt cggggaaccg
      481 ctcgcgcagc tcgcgcttga gcaccttgcc cgtcgggttc cggggcaggt cgtcgacgaa
      541 ccacaccgag cgcggcacct tgtagttggc caggttcgcc ttgacgtggt ccttgacggc
      601 gcggtcgtcg aggtcgacac cgtcctcggt caccacgaac gcggcgaggc gctggccgaa
      661 ctggtcgtcg tcgacaccga gcaccgcgac ctcgcgcacg ccctccatgt ccgagagcag
      721 gtcctcgacc tcgctcggga acaggttctc gccgcccgac acgatcatgt cgtcgtcccg
      781 accgccgacg aacaggcggc cgtcggtgtc gaagcgcccg aggtccccgg aggacaggag
      841 cccgtcgatg gccggcttgg tctgcccgcc ggtgtagccc tcgaagcgca gcgcgctccc
      901 gacgaagatc cgcccgacgg tgtcgggtcc ggtgatccgg cgatcgtcgt cgtcgtagag
      961 ccggacgtcg accccggcca gcggcttgcc cgcggtgccg ggcgcggccc ggaggtcctc
     1021 gggcgtggcg atggtggcca ccgcgacctc ggtggagccg tacatgttgt agaggttgtc
     1081 gccgaagcga tccatccact gcgtggcgag cgacccgggc agcgccgaac ccgagcagaa
     1141 cacgacctcg aggctggagg tgtcgtagcg ctcggtgact gcttcgggca gggccacgat
     1201 ccgctgcagc atcaccggca cggcggtgac gacgcgcacc cggttcgcct cgatctgggc
     1261 gagcaggtgc tcgggatcga accggcgcga cagcacgagc gtgtgggaca tggtggcggc
     1321 ggcaaggagc gaggcgttgc cgagcgagtg gaacatcggg gccggcacgg cgtgcacgtc
     1381 ctcggtgcgg aacggcaccc gctggaagat ggcggtggcg gcgaccagcc cggcgcccag
     1441 gctctccttg ctgcgcgacg cgcccttcgg cgtgcccgtg gtgcccgacg tgaggatgac
     1501 ctggcgtccc tggcgctcgg gcttgtccgg gtcctgcgtg gaggtcgcag ccacgagggt
     1561 cggcaggtcg tcggtgccgt cggccggggc ccggtcgcac accagccgct gcacgtccgg
     1621 ggcgtagccg gcgacggcgt cgctgaactc gtcgtcgtgc aggagcaggt cgcagccctc
     1681 ccgctcgagc acctcggcca gctgcgggcc agcgaagccg gtgttcatgt acaagacggt
     1741 ggcgccgagc ttgtcgagcg cggccacggc caggacgaac cagcggctgt tgcgggcgag
     1801 cagcgccacc ggcgtctccg gtccgacgcc acgtcgggcg agcgcgttcg ccagcgcgct
     1861 cgactgctcg tggacctcgc ggaaggtgag ggtgccgtcg tcgtcgacga tggcgacctc
     1921 gtcaccccgg cgggcgacgc cggcggcgta gcccgcggcc agggtcggcc cccaccgctt
     1981 cgccgactgc agggcgctgg ccgccacctt cggcttcagc aaacccatcc ggcggatggt
     2041 gtccaccgcc tggaggttga ccgagacgcg atcgaagagg gatgccatgg gaggaatcgt
     2101 aggtttcggt cagctgatcg acagcaaggc caggaacgcc gtcgcccagg cggcgaggtc
     2161 gttgcgttgc acggtgtcgc gcatcacccg catgtgcgcc cgctgctcgt cctcgtccat
     2221 cgacacggcg gcggcgatct gctccttgag gtgctcggtg tcgtaggggt tcacgaggaa
     2281 cgcggcgttg agctcctcgg cggcaccggc gaactccgag agcaccaggg ccccggtgtc
     2341 gtcgacccga gccgacacgt actccttggc caccaggttc atgccgtcct ggaacggcgt
     2401 gacgagcagg acgtcggcgg cccggtacag gacgagcagc tcggcgaacg gaaggctctt
     2461 gtggatgtac tgcaccaccg gacgcccgag cgtgccgtag tcgccgttga tcgtgccgac
     2521 gagctgctcg acgcggttgc gcaccgagtc gtagccctgc acgtcctcgc ggctcggcac
     2581 cgccacctgg atcacggccg tggaccgcgg gtcgaggcgg tgctcgatca gcagctcgtg
     2641 gatcgccttg aggcgcaggt cgatgccctt ggtgtagtcg agccggtcga cgccgagcag
     2701 cacgtgcttg gggtcgccga gctccttgcg cagctccttc accgcgagct cgacctcgtc
     2761 ggaggcggcc tgctcggcga tcgcctcgta atcgacggag atggggaacg ccttgaccac
     2821 cgaggtgtcc cggccggtgc ggaccacacc gccgtcaccg gcgaaggtga ggtcgtcgac
     2881 ctcgcagaac cgccgcacgc cgtagagcac gttgcgggcg tcggcctggg tctgcacgcc
     2941 gatgacgtcg gcgccgagga gcccggcgag cagctccgcc cgccacggca gacgggagaa
     3001 cagctcgagg ggcgggaacg ggatgtggag gtagaagccg atgcgcacgt ccggtcgctg
     3061 ctcgcggatc atccggggca ccagctgcag ctggtagtcg tgcacccaga ccgtgccgcc
     3121 ctcggccagc tccgcgcacg ccgcgtcggc gaagcgctgg ttcaccgtcc ggtacgccgc
     3181 ccaggtctcg cggtggaact gcgtggtctc gatcgcgtcg tggaacagcg gccagatcgt
     3241 ctcgttgcag gcgccgccgt agtactcctc gcgctcctgg tgggacaggg cgagggccga
     3301 gagcgtgatg ccctcgaagt cgaagcgctg ggcttcaccg tcgtcgctgc cgtcccaccc
     3361 gacccaggac ccggtccggt cgcgcatcac cggcagcatc gccgacacca gcccgccggg
     3421 gctgagggcc caggagccgt cgtcctcgag gcgcaccggg aggcggttgg cgacgaccac
     3481 gaagtcggcc tgaccggccg gaggcgtgtc gggcgcggtc tggtcgggcg gggtctggtc
     3541 gtgctccacg tcgggctccc tcgagggtgt ctgttcgctc acgatacgtc gctcgcgctg
     3601 gcgcttggct tccttccccg ccgctcgggc accgatgcca gcaggaccga tccggaggcc
     3661 ggccacatgg ggtctccgcg gatcgggtag gaaggccagc gctgtcccca cccctgaacc
     3721 ctggaggcga ccccgtggga atcatccgca agacgctgtc ggtcacgacg ctcggcctcg
     3781 tcggctggaa gtccaagaag cagctgctgg ccgaggccga ggccgacctg gcatcgaccc
     3841 gcgccgacct ggagcagacc tcggcgctgc ggtctgcgct gcaggaacgc ctccagaacg
     3901 tggagcacga cctcgagagc agcgagctcg acgcgctgaa gggcgcgaag gttgcccgca
     3961 aggacgagcg ccgccggatg cgtcgcgaga aggccgaggc ccaggccgcc cgcgcccgca
     4021 agcaggccga agccagggcc gaggaggccc gcgcccgcct gcagaagacc tcgaagtcgg
     4081 cccgcaaggc cgccgaacgg gcccgcaaga aggccgagca cctggccgac gacgcccgcc
     4141 accaggtgga cgacgtgacc aagaccgtgc gcaagaagcg caagaagctg cggggctgaa
     4201 ccccgcagcg gctcaccgag cctgcgggtt cggcaccggc gggcggggcc ggcccggcag
     4261 cggatccgga gacggctgcg ggtcggggtc cggtcccgga tcgggcaccg gcggccgggg
     4321 gtcgggcggt tgcgggcccg ggccggggtt cggcgtcggg tcgggcacgg gcgggcgatc
     4381 gggatccggg ccgggcatcg gctcgggcgt ggtggggtcc ggcgtggtca tcgtcgcacc
     4441 accagcaccg ggcacggagc gtgccggacc acgtggtccg acaccgagcc gaaccacagg
     4501 cgtgaccagc ggctgcggtc gtgcgtgccg agcacgacga ggtcgacgcc ttcgtcctcg
     4561 gcgaccttgc agatggtcgg cccgacatcg ccgtgctcga cccgccgctc gtcggcgtgg
     4621 agcgcctcgc cggcgcgctc gacgacctgc tcggcctccc ggtcggccat ctcctgggcc
     4681 cgcacgtcgg cctcggcgct gagcgtgggg ctgccggcgt aggcggcgcc gagcccaccg
     4741 atggtgccgt acggcgtgat ggtgctgcgg gccacgttca cgacgatgac cgcagcatcg
     4801 tcagggacca actgctgggc gaaggacatc gcgtcctccg actccggctc gtcgtcgacg
     4861 gcgatcaaga ccttcatgcg cgctccctac cccggtgcgt ggttccgatg ccgtcagcct
     4921 ggcgcatcga cggcgtggat gccgtcctgg ccgagcacga gcagctcgcc gtcctccagc
     4981 tcggcgaagg acagcaccgg gccgaagccc gagacgagct ccaccggctg cgcccccggg
     5041 tcctcggccg gcacggcccg gacccacccg ccgcagaagt cggcgaagag gtaccacccg
     5101 tacagcgacg ggaccgcctc gccccggtac acgtacccgc cggtgatcga gcacccctcg
     5161 tcgtgggtgt actcgagctg ggggtccgtg agctcgggtc cgggctcgcc ggcgagcggc
     5221 cgcgtgccct cgagccggtt ccacccgaag ttcacccggt cgtcggcacc gggatcgacg
     5281 acggtgacct cctcccgggc gttctggccg acgtccgcca cccagagctg cccggtggcg
     5341 cggtcgaacg agtaccgcca cgggttgcgc agcccgatgg cccagacctc gggccgctcg
     5401 ccccactcgg ggttcgaggc gggggcggac acgccgccgg cggggtcggg gacgacgcgc
     5461 agcatcttgc cgagcagcac gtcggggtcc tgggccaccc cggcggggtc gttggcgctg
     5521 ccgccgtcgc cgacgccgat ccacagggcg ccgtccgggc cgaacaccag gttgccgccg
     5581 ttgtggttgc gatacggctg gccgatctcg aggtgcagca ccgaggcctc cacgccctcg
     5641 gccggtcgat cgccgtccat ggccaccgag cgcacctcgg tgtcccccgc gctgttggtg
     5701 tagttcaggt acaaccgatc accggacggg tccagcgcca gcccgaggag cccgcgctcg
     5761 ccgcccgtcg acacccggtc ggacaggtcg aggacgacct cgctggtccc gccgtcgaca
     5821 tcgacggcgt ggacccgccc gtcgagcgtc gacaccagga tcgggccggg tccgtcgggg
     5881 tcgaccgcag cggagggccc cgggaggtcg acgaccggcc ccagcgcgat ggccgggtcg
     5941 gtgccggtct cgagcggagg ttccgtcgtc gtggacgtgg cctccgtggt gttggtcgtg
     6001 gtcggctcga actgcgtcgt cggggccggc gcgctggtcc ggtcctcggc gtcgtccacg
     6061 atcccgtcgg ggaagacgta gccgtacacg tcgccgagct gctggcggaa ggcgaccccg
     6121 aggatcacca cgagcacgag cgctgcgacc aggccgatgc ggatcgggcg ggacgtcggc
     6181 agcaggcgca ccggacgatc atggcaaccc gggaaccgat gccggtcgcg gcccggacgc
     6241 ggaagagccc ggtggcggtg ccaccgggct cctcaggctt tcgaaccagg cccgctcagc
     6301 gggtcggggt cagctggtgc ggctcacctt gcggaggtgc accccaccgg cgaccgacag
     6361 gaccagcatg accgctgctg cagcgaaggc ggcagttgcg gcgatgccgg cgatctggcc
     6421 catcgtggac caggcgtagg cgttcaggag cgtgcctcgc agcatctcgc ccttgaagac
     6481 cgtgtcacgc tgctggctga gggtggtggc ttcctgctcc agctcggcga tctcggcctc
     6541 gggagcgccg ctgtcgatgg cctcctgcag ggcggtgcgg gcctcgcggt ccgggatctc
     6601 ggcgtaggtc aagccgccag ccgtggcctc gacgtggccc tggatgaagc tggcgtaggc
     6661 ctcggcctct tcgccggtgt cgacctgctg gccgccgtac tcggcgaggt cctcacgtcc
     6721 gtcctcgatc agcgcctcgg tgggcgggaa gctgatgttc tgtgcggcga gctcgtcgcc
     6781 gacgtagtcc tcggcgaagt tgctgcccca ggtgagcagg ccgccggcga tcgccagcac
     6841 gatggcggcg acgacgccgg caccgatgag gaggtggtcg atccgacgcc gggtcaccgg
     6901 aatcggcttg tcgaccggag tcttcggggt ggttgtggtg gaggagcgat cctcggtcat
     6961 cgtcatggtg gcgtcctttg cctctggttc cgttgcccga tcgggcatgt gttgaaccat
     7021 acggacggcc ctcggatccc accggggccc aaggtcacac gacgccgggc cattcacgcc
     7081 cggtccgtgg gacgtttgcg accccgttcg cccccggatc agccgccgtc gcgcagcgcg
     7141 gggagcaggt cctcccgggc gaagcggaag aacccctccg acgtctcccc gatctgggac
     7201 acgaagatct cgtcgtagcc ggcctcgtcg aactcgcgga tcgaggccag gtgctcgtcg
     7261 aggtcggggc cgcaggcgaa cttctccgac accatctcct cggtgaccag cgcgccggcc
     7321 tgctcgaagt gggcgggcat cggcagctcc tgggacgcct cgcccggcac gccgttgggt
     7381 ccccacaggc gatgcgcggt cttcacgcac tggtcgcggt cggaccccca gcacaccttc
     7441 accccggcct gggccggacc cgagccccct tcgctgcggt actgctcgac cagctcggca
     7501 tcggggctgg tgccgcagta gccgtcggcg acccgggcgg cgagcgacac cgccttcgga
     7561 ccgaagccgg agacgtacac gggcgggggc gtgtcggggt tggagtacag gcgggcgttc
     7621 tccaccgtgt agtgccggcc atggtgggag acctggtcgc cggtccacag cgcccgcatc
     7681 acctcgatgg cctcctcgag catctcgagg cgcacgtcgg cgggtggcca gcggtcgccg
     7741 aggacatgct cgttgaggtt ctcgcccgtg ccgaccccga ggttgaagcg gccatcggtg
     7801 agcagggcgc tggtcgccgc ggcctgggcg atgatcgccg ggtggatccg gatcgtcggg
     7861 cacgtcaccg cggtggtgat cgggagcgag gtcgcgttgg cgatggcacc gatgacgccc
     7921 cagacgaacg ggctctcgcc ctgcgcgtcg ttccaggggt ggaagtggtc cgagatccag
     7981 agcgcttcga acccggcctc ctcggccagg acggcctggc ggaccagctc ggtcggggtg
     8041 tgctcctcgc tggacaggaa gtagccgtac gtcgtcatcc cgtgcctcct cgtcgtggcg
     8101 gtcgtccccg gtcgctaccc gcggccgacc tcgctcagga ccggagcagg tccaaccgct
     8161 cggcgacgag cgggctcgcc aggtgggcgc gcacgctggc gtgggccacc tcggtgatgt
     8221 ggcggcgccg ggcgggatcc atcgagctcg gaccgacggc ggccaccacc tcgggaccgg
     8281 gctgcagcac caggacgcgg gcgccgccac gacgcagggc gcgcacctcg cgggcgaggg
     8341 tcgcccggtg cagtggcctt gcgccggtcc acccggtccg cagcgcggcg cgggtgcccg
     8401 acatcggcga ggagatcacg acgatgtcga agccgagctc ggcggccagg tccgcgttcg
     8461 tgggtgagtg gacgccgccg tcgacgtagg tggcgcctcc gtgctcgacc ggcgcgaagt
     8521 accccgggat cgccgacgat gcctccaccg cggtggcgag gtcggcgtcg tcgacgtcgc
     8581 gaccgaagac ggtccggcgc ccttcgggca tccggaccgc gcagatccag gtggtccggt
     8641 cgggccagcg gtcgtcgtgg gtggcccgga tgcggtcgcc gaggacggcg gtcggcacgc
     8701 ggcccgtcgg cagcagcccg gccacggcca gcatcgggtt ggcgcgccac ggccgggcga
     8761 gcgcccgcag cgccaggagc ggcgccaccg gccgcgccca cccgagcttc ggcgccggca
     8821 ggtccatcgg ccctccggcc cgggcggcgt gcgccgcgcc ctgcgggctc agcggacgac
     8881 cgagcgcgcc ggccaccagg tcgctgggtg gcaccccgag gcgcaggacc gcagcgacgt
     8941 tcgaccccgc cgaggtgccg atcacgaggt cggcgcgccg ggcgtcccat cccacctcgt
     9001 ccagggcggc gaggaccgcc ccgtgccagg ccgcgccggc gatgccgcca cctccgagca
     9061 cgagcgccac cgagaccatc ggcgcagtct gccgaggaca gacaggatgg cgtcggtgga
     9121 cctcatctcc gagcaccccg tgctgctgct gttcctcgtg atcggggtcg gctcggcggt
     9181 cgggcgcctc cgggtccgcg gcgtcgggct cggtccggcg gccgtgctct tcaccggcct
     9241 cgccgcctcc ggcatcgacc cggacctcgt gctctccgag gaggtcatgg agctcggcct
     9301 ggtgctgttc acctacacgg tcgggctggc cacgggaccg gcgttcgtcg ccgggctgcg
     9361 gggtcggggc ctgcggtcgc tcgcggtcgt cgccatcgcc accagcgccg cggcggtcgt
     9421 ggccgccgcc gccggggtgg tgctcgacct cgggtccgtc gaggtcgccg gcctgttcgc
     9481 cggcgcgacc accaacaccc cggcactcgg tgccgtgctg gacgccgccg gccccgaagg
     9541 gcccggcgcg gcgatcacct acgccatcgc ctacccggcc ggggtcctgg tcgcgctgct
     9601 cgcggcgtcg gcggcgctgc ggaccggccc cgctccggag caggacgacg acatcgtggc
     9661 gtgggccgtg ctcgtgaccc gcgatcgggt cgcgctcgga cagctcccgt ccggtgtctc
     9721 cttcggccgc tacatccggt cgggcgccag cgaggtcgcc acgcccgaca ccgtggtggt
     9781 ggagggcgac gtggtgaccg tcgtcgggcc cggaggcgag gtcgccgcgg tggcggccgc
     9841 gctcggtgac cacgtgcaga agggcggggt ggtgctcgac cgatcgaccg tggacttccg
     9901 ccgcatcacc gtctccgacc cggcggtgat cgggcggcgc ctcggtgagc tggacctcag
     9961 cggtcgcttc ggggcgacgg cgacgcgggt gcgccgcagc gacgtcgaca tggtcgcccg
    10021 tccggacatg gtgctcgagg ccggggaccg ggtgcgcgtc gtcgcgccgg tcgccgagct
    10081 gcacgcggtg agcgcgctgt tcggcgactc cgagacccag ctgaccgaca tcgacttcgt
    10141 cgcgctgtcc gccggtctgg tcgtcggcgt gctgttcggg ctggtcacgt tcccgggcgg
    10201 cctccggctc ggcgccggcg gcggtgcgct gctggccggc ctggcgctgg gcgcggcggg
    10261 gcggctcggt ccggtcagct tcagcatcgg ccaccaggtc tccaccggcc tgcgccacct
    10321 cgggacggtg gtgttcctcg ctgccgtcgg cacgcgctcg ggcgaggcgt tcgccgcatc
    10381 cgcgttcacc gtggacggcg cggaggtcgc ggccgcctcg gtcgccgtcg tggccgccgc
    10441 ggtgctggcc ctgcggtgga cggtcggccg gctggcccac ctcgaaggag cggcggcctc
    10501 cggcgcggtc gccggtctgc agacccagcc cgccgtgctg gccttcgccg actcctcgag
    10561 cggccacagc ccgcaggtgc ccctcggcta caccgccctg taccccgccg ccatggtggc
    10621 caagatcgtc ctcgcccagc tgctggtccg gctgtgagcg ggtgcgctcc aatggtcacg
    10681 ccgtcccccg ccaggagcct ccgatgatct tcgccaccga cgccgccaac aacgccgccc
    10741 gccgcttcga gcacatcgag gtgcgccccc tcgccggtgc gctcggtgcc gaggtgttcg
    10801 gccccggtgg tgcgcccctc gacgtgacgg cgctgagcga ggccgccgtc gccgaggtgc
    10861 gccaggcctc ggtcgaccac ctggtcgtct tcttccggga ccagtccctc gacctcgact
    10921 cgttctccgc gttcgcctcg acgtggggtc cctacgggga cgatcccttc gtggaggggc
    10981 tcgagtcgca tccgcacgtg gtcaaggtcg tgaaggaggc ggacgagaag gtgccgctcg
    11041 tgttcggcgg tgcgtggcac tcggactggt cgttccaggc ggagccgccg gcgttcacca
    11101 tcctctacgg ccacgacgtg cccgaccacg gcggcgacac gctctgggcc aacctctacc
    11161 tcgccgccca gcacctgtcg cccggcctgc gggcgacgct ggccgacctg cgcgccgtgc
    11221 actcgccgca gaaggcgtac ggcgcccagg ccacgcacaa cgacctcgtg gagcacatgg
    11281 ccatccgcta cggcgaggac gcccacctgc gacgggccca gccgatcctg cgccagcacc
    11341 cggactccgg acgcacggcc ctcttcgtca actggggcta caccgagtcc atcgagggct
    11401 ggtggccgga cgagagccgg gcgctgctcg accacctctt cgaggtctcc cagcacccgg
    11461 cctacacgtg ccggttccgc tgggagccgg gttcgatcgc ggtctgggac aaccgctgca
    11521 ccctccacaa ccccatcgcc gactacgccg gccagcgccg cgagatgtgg cggatcaccg
    11581 tgcgaggcga ggagccgatc cccgccgggc acccgtagcc tccgtcccat gcccatcccg
    11641 ttccccgcac cggaccgcac ctgcatcgtc acgggcgcgt cgtcgggcat cggcaccgag
    11701 atcgcacgcg tcctggcgtc ccgcggccac ggggtcaccc ttgtcgcccg ccggctcgac
    11761 cgcctggaga ccctggccgg ccagctgtcc gagcgccacg gcatccgggc cgaggtggtg
    11821 gtggccgacc tgaccgagcc ggcgcagcgg cgcgccgtca tcgaagccgt ggacgagcgg
    11881 ggcctgacgc ccgaggtcct cgtcaacaac gccggcttct ccacggtcgg gcccgtcgtc
    11941 gacgtcgacc cggggcgcga gatccagatg ctgcgcacca acgtcgaagc ggtcgaccac
    12001 ctgagcgcgc tggtggcacc cggcatggtc gagcgcgggc gcggcgccat cctcaacgtg
    12061 gcctcgaccg cctcgtacca gccgttgccc ggtcaggccg ggtacgcggc cagcaaggcc
    12121 ttcgtccgct ccttcaccac cgcgctcgcc ggcgagctcg ccccgcacgg cgtgacggtc
    12181 accgcgctct gcccgggccc ggtgcggacc gagttcggcc acgcatccgg catggacgac
    12241 ttcaccgacg gcaaccccct ccccgaggcg atgtgggtgc cggtggagca cgtcggacgg
    12301 atcgccgtcg atgcgctgga ggacggccgg ttggtctgca tcccgggtcc gctcaaccgc
    12361 ctcgtcgccg ccgtcgccaa cgtcacgccc cggcaggtgc tgctgccggt gctggtccag
    12421 cgccacccgt cgctgcgatg acctctccgc accgccgcgg gcaggccgac ctcgacctgc
    12481 tcccggtcgg cgtcgcccac gtcgatcccg cggggaccat cgtccgggcc aaccggatga
    12541 tcggcgagct ggtcggcgtg ccggcggaca cggtggtcgg ccggagcctg ttcgagttcg
    12601 cagccggtga catggaccgc tacgccgcga tgctcgactt cagcaccggc tacgacgccc
    12661 tgatgggccc cgtcgccgtg acgtaccgcc gggccgacgg cagcctccgc cagagcgccc
    12721 tgtgggccag gaaccacctc gacgaccccg acgtcgccgc cctcgtgtgc acgttcgtcc
    12781 cggaggacac caccggtggg ctggccgccg cgctcgcctc ggtcgcggag ggcgacgacg
    12841 tggacgacac cctcgcgctg ctctccgccg ccctgcgcgg caaccccttc gaggcccgtg
    12901 ggtgctggct cgtgcgcgac gggtcgggcc gacgcctcgc cggcgacgcg gacctgatcg
    12961 acgcggcgcg cgctgccctg gcccagcccg gcgcgtggtg gcgagcgacc cggggcaccg
    13021 gtctggtgac ctgcgacgac acgcgccacg gcgacgagta cgaccggctc ctctcggcgg
    13081 cgggcatcgt cgcctggtgg gccctgccct gccagggctc cgacgatgcg gaggcggcca
    13141 tcctggtgct gcgcacggag ctcggtccgt tgagcccgct gcagcacgaa cagctcgacc
    13201 agctcgcgac cacggccgga ctggcgttcc agcgggcggc gatgcaggcc cgactggccc
    13261 acgccgcctt ccacgacccg ctgaccggtc tggcgaaccg cggtcggttc ttccaccgcg
    13321 acgacgccag gctgcaggcg ggcgcggccc tgctctacgt ggacctcgac cggttcaagc
    13381 cggtgaacga ccgcctcggt cacaccgcag gcgacctggt gctcgtcacg gtcgccgacc
    13441 ggatccgccg agcggtgcga cccagcgacc gcatcacgag gttcggcggg gacgagttcg
    13501 tggtggagtg cgccggcgtg gccaccgacg acgaagcggt ggtgatcgcc gagcggatca
    13561 tcgctgccat ccaggagccg atccgcctcg acctgggcga gatccagatc ggcgccagca
    13621 tcggcatcgc ccggaccgac gaggccgtcg gcgtcgacga cctgctcgac cgctccgatg
    13681 tcgcgctgct cgcagccaag gccgccggca agggccggtg gcacctcgcc ggccccggat
    13741 gatcagccgc cggtgagctc gtcgagcacg gcgtcgacgg ccgcgaccgt ggcgcccggc
    13801 tcgtggacga tgcggctcag gtagctgatg gacacctcgc cggcctccac ggagccgccg
    13861 tcggtctcga tgggcagctc gaccgggtcg ccccactcca tctcgacggt gaaggtctcc
    13921 tcgtagccct cgcggggcac gagggtggcc gaggacatcg accggggcgg gatcgtgccg
    13981 acctcggcgt ggcgccacgc cgtgatctcc tccagcccga cgttggggac gttgatgttg
    14041 accacgaggg gcgggtcggg caggtcgtcg atcagcgacc cggtgacccg ggccgcgacc
    14101 tcggcggcgg tgtcccaccg ctggtcctgg atcatctcgt cccagccctg gccctcgacg
    14161 ccgaacccga tcaccgactg gctcaccgcg atgccgttca gcccgccgtt gcgagccgtg
    14221 atggcagcgc cgacggtgcc cgagtggtag atcgcccggc cggtgttggc gccggggttg
    14281 atgcccgaca ccaccacgtc gatgtccccg aagcagccga agcgagcgaa gtacacgcac
    14341 agggccggcg ggccgctcac cgaccacgcc tccggcaccc cgtcgatgtg ctcccgctgc
    14401 acttcgggct gcatgaggtg cagcgtgccg agcgccgcgc cgaagcccga gtactcccgg
    14461 tcgggcgcga cgaccaccac gtcaccgagc tccgtgagcc ggcgggcgag gacgtgcagg
    14521 ccgagcgagt cgatcccgtc gtcgttggtc acgaggatgc gcaccgcctc cttctaccag
    14581 ccttccggtg gatcgccgat ggccgcgagg tggccgcccg gtggcttcag cagccgccgc
    14641 ccccgccggg ggacggtcga tccgggatgc tgatgtccac gacctcgatg tggacgtggg
    14701 gccaggcggg cgacgccgtg tggtcgtcga cctgggatgc gaaggggagc agcgtgggtc
    14761 ggggggcgag caccgtcgca ccggcctcga cccgctgccc ggcctgcacc tgcacgccgt
    14821 cgatgtgcag gaccttgacc tcccagccgg ggcgggcgtc gggttcgatg accgcgtagt
    14881 cgtcctcgtg gtcgcagtag aggatgtagc cgccggcccg cttcacggtg ccggtgaccg
    14941 gcgagcggat ctcggcgtcg ggggacacga cgatgtcggc ggcgctgcga gaaccggtgc
    15001 ctcgctctcg cgactcgagc tcgagggggc gggccgcctc cgcggtgggg tccatctgcc
    15061 gcgcgccgtc gtggttggac tcgtggaagc cgaccgtctc cacctgggcg gcggggtggg
    15121 cgagcacgac ctccccgacg gtggcgaacg gcgcgaaccc ggtgtcgacc cccacgcgcg
    15181 ggatcgccgc cgtggtcggg ggcgcagccg tggtcggcgg cgccgcgctg gtggggggtg
    15241 cgagcgtggt cggcaccgcg gtcggcgtgg gatccggggg tgcctcgacg gcggagtcgg
    15301 ccggcagcgg tgcggcccca ccggcgcgct cgacggggac gtcgtcaccg ccgcaggcgg
    15361 cgaggaccag gcagagcgcc agcgcgagac gggccgggat gcgcaggggt tcagtcgagg
    15421 ccggggccct ggcggcggtc gtccg
//
LOCUS       DATISU010000212         4552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_150212, whole genome shotgun sequence.
ACCESSION   DATISU010000212 DATISU010000000
VERSION     DATISU010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150212"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VLR27_08865"
     CDS             <1..1078
                     /locus_tag="VLR27_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP03597.1"
                     /translation="FPEGWGGMGLAPTHQRIVEKALREAGGPGMDQTQFFGLALAGPT
                     VVSHGSEDLKSRTLRRMYTGEDAWCQLFSEPGAGSDLAGLSCKAVRDGDEWVVSGQKV
                     WNTMAHLANKGMLVARTDPDQPKHKGLTYFMVDMTAPGVDVRPLRQITGEAEFNEVYL
                     DEVRIPDADRIGDIGDGWRVSMTTLMNERTSIGGGGGGGPRPAGSGPIAEAVRIWNEL
                     PEWARTDAHRDRLMQLWVQAEALRLTNIRAGQARKAGNPGPESSISKLMLALVNKDIY
                     AYCVDLLGAQGLIDYDFDMRRADAIGLEGPPGSARKMFLRSRANSIEGGTSEIMKNIL
                     GERVLGLPGEPRVDKDLPWKEVPR"
     gene            1129..1767
                     /locus_tag="VLR27_08870"
     CDS             1129..1767
                     /locus_tag="VLR27_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03598.1"
                     /translation="MRLLILCFAALALLAGACGDDDDSATGTTTTLGDVTSTTADATS
                     TSTTTSTSVPSSTTTEDEGTASLEQTCESPDGFTISYPGDWDAVSDCGQFGPAPLDEP
                     APATDERTGVVSAFVDPVPFEQVAEPAEGDDAREETTIDDLPAVRVEGEQEAEGLYPE
                     GTSYVRWMVDLSEVRGDATLFLDAYDLGYDIDFGQAVQVLDDMVETVEVTDG"
     gene            1807..2919
                     /locus_tag="VLR27_08875"
     CDS             1807..2919
                     /locus_tag="VLR27_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03599.1"
                     /translation="MRDRTTLRILLLAVVGTAFTVALLAHDLSEADHISNVLLAGEEG
                     PSVDAVTADIPDADLAPERGHDGQQFYAIARAPMHLDEASEHLDRPRYRLQRPLFSVL
                     AWALHPSGGGTGLVVALLVVGVAGVLLSGVAAGALSHTLGGGTWPALLVPVLPGAFGA
                     LRITAADTLALGLLLAAVWAAEIRRSRLGVAAAVAAVLAKESMALLLVAHAATRRTRE
                     SVLAALAGVASAGTWWVALRLLVDSDVAQVKEFTWPFGGMIVYVDEWLSGHNLIGAAM
                     AIGSFLLAFVALWRRGPRHPLFGTLAAMTALASVFAASVVALDYNAIRTLGPLLVIAI
                     LTLGTPTPTPAPGHDGAADPGARRVLVDPGPVRRVG"
     gene            complement(2903..4237)
                     /locus_tag="VLR27_08880"
     CDS             complement(2903..4237)
                     /locus_tag="VLR27_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03600.1"
                     /translation="MTDLLEPGVDPDLALKALEAAVGMVQAVDLDGLSPAEELALVGR
                     LEKLRRRLDHGTDLTIAHLDTSAAFSVDGHKTAKGALKAIGRLSGSDAHGRVQTSRAL
                     SRLPLVEAAYARGEIPTEHVRAMARTISNPRVAEWVPEADPIFAHHATVLGYDDFCAA
                     LRQWEALADADGADQAAERSHERRNASVQESAIDGSFRLESNHGALQGAAMAEIFDRF
                     ETAELHADWAAARELHGDDARVEHLDRTPAQRRADAMFRIFCRAAAAGGATPEPLVNI
                     VVDQDTFEGELARSAGVPVTVDPNAALDGRRCHTVDGTQIRPAEAVAAALVGHVRRVV
                     VDGAGNVIDLGRRQRLFTGSARDAAFLQALLRGPGGLGCLWPGCDGRGKNLQVDHRDP
                     ACRGGPTNTDNSDAYCGFHNRTKEHGFQPVRDPDGTWTIHRPDDDGPITPPV"
BASE COUNT          632 a         1642 c         1606 g          672 t
ORIGIN      
        1 cttccccgag ggctggggtg gcatggggct ggcgccgacg caccagcgca tcgtggagaa
       61 ggcgctgcgc gaggccggcg gtcccggcat ggaccagacg cagttcttcg ggctcgccct
      121 cgccggcccc accgtcgtca gccacggcag cgaggatctc aagtcccgga cgctgcgccg
      181 gatgtacacg ggcgaggacg cctggtgcca gctcttctcc gagcccggcg ccggttccga
      241 cctcgccggc ctgtcgtgca aggccgtccg cgacggcgac gagtgggtgg tcagcggcca
      301 gaaggtgtgg aacacgatgg cccacctggc caacaagggc atgctcgtgg cccgcaccga
      361 ccccgaccag cccaagcaca agggcctcac ctacttcatg gtcgacatga ccgcccccgg
      421 cgtcgacgtg cggccgctgc gccagatcac cggcgaggcc gagttcaacg aggtgtacct
      481 cgacgaggtc cgaatccccg acgccgaccg catcggcgac atcggtgacg ggtggcgggt
      541 ctccatgacc accctcatga acgagcgcac ctccatcggt gggggcggtg gcgggggtcc
      601 ccgcccggct ggctccggcc cgatcgccga ggccgtgcgc atctggaacg agctcccgga
      661 gtgggctcgc accgacgccc accgggaccg gctcatgcag ctgtgggtgc aggccgaggc
      721 gctgcgcctc accaacatcc gggccggcca ggcccgcaag gccggcaacc ccggcccgga
      781 gtcgtccatc tccaagctca tgctcgccct ggtgaacaag gacatctacg cctactgcgt
      841 cgatctgctc ggcgcgcagg gcctcatcga ctacgacttc gacatgcgtc gcgccgacgc
      901 catcggcctc gagggccctc ccggatcggc ccgcaagatg ttcctgcgct cgcgtgccaa
      961 ctccatcgag ggcggtacgt ccgagatcat gaagaacatc ctcggcgagc gggtgctcgg
     1021 cctgccgggc gagccccggg tcgacaagga cctgccctgg aaggaggtcc cccggtagct
     1081 cgccggcgga ccttctacgg tccctgccga ctcggttagg gaccacacat gcgactcctg
     1141 atcctctgct tcgccgcgct cgcgctcttg gccggggcct gcggtgacga cgacgacagc
     1201 gcgaccggga ccaccaccac cctcggcgac gtgacgtcga ccaccgcgga cgccaccagc
     1261 acgtcgacga cgacctccac gtcggtgccg tccagcacga cgaccgaaga cgagggcacg
     1321 gcctcgctcg agcagacctg cgagtccccg gacggcttca cgatctcgta ccccggggac
     1381 tgggacgccg tcagcgactg cggccagttc ggtccggctc cgctcgacga gcctgcgccg
     1441 gcgaccgacg agcgcaccgg ggtcgtgagc gcgttcgtcg accccgtccc gttcgagcag
     1501 gtggccgagc cggccgaggg tgacgatgcc cgcgaggaga ccacgatcga cgacctcccg
     1561 gccgtgcggg tggaggggga gcaggaagcc gaggggctgt accccgaggg gacgtcctac
     1621 gtccgctgga tggtcgacct gtccgaggtg cgcggggacg cgacgctgtt cctcgacgcc
     1681 tacgacctcg gctacgacat cgacttcggc caggccgtcc aggtgctcga cgacatggtg
     1741 gagacggtcg aggtcaccga cggctgaggg gttgccgtcg ctgcatccgg gtaccggtag
     1801 ccgcacatgc gcgaccgcac caccctccgg atcctgctgc tcgccgtcgt cggcaccgcc
     1861 ttcaccgtgg cgctgctggc ccacgacctg tccgaggccg accacatctc caacgtcctg
     1921 ctggccggtg aggagggacc gtcggtcgac gccgtgaccg ccgacatccc cgacgctgac
     1981 ctggcacccg agcggggcca cgacggccag cagttctacg ccatcgctcg cgcaccgatg
     2041 cacctcgacg aggcgagcga gcacctggac cggccccgct accggctgca acgtcccctc
     2101 ttctccgtgc tcgcctgggc gctgcacccg tccggagggg gcacgggcct ggtggtggcc
     2161 ctgctggtcg tgggcgtcgc cggcgtgctg ctcagcgggg tggcggcagg tgcgctgtcg
     2221 cacaccctcg gaggcggcac ctggccggcg ctgctcgtcc ctgtgctgcc cggcgcgttc
     2281 ggcgcgctcc ggatcaccgc ggcggacacc ctcgccctcg ggctgctcct ggctgcggtg
     2341 tgggctgcgg agatccgacg ttcccgcctc ggggtggcag cggccgtcgc cgccgtgctg
     2401 gccaaggagt cgatggcgct gctgctcgtc gcccacgccg ccacccggcg gacccgggag
     2461 tcggtgctgg ccgcgctcgc cggcgtggcg agcgcaggca cgtggtgggt ggccctccgc
     2521 ctcctcgtcg acagcgacgt cgcgcaggtc aaggagttca cctggccctt cggggggatg
     2581 atcgtctacg tcgacgagtg gctcagcggc cacaacctga tcggtgcggc catggcgatc
     2641 gggtcgttcc tcctcgcttt cgtggcgctg tggcgccgag gaccgcgcca cccgctcttc
     2701 ggcacgctcg ccgccatgac cgcgctggcc agcgtcttcg ccgcctcggt ggtggcgctc
     2761 gactacaacg cgatccgcac gctcggtccg ctgctcgtga tcgccatcct caccctcggg
     2821 acgccgacgc cgacgccggc tcccgggcat gacggagctg ccgaccctgg ggctcggcgc
     2881 gtgctcgtcg accccgggcc ggtcagacgg gtggggtgat gggtccgtcg tcgtcggggc
     2941 ggtggatggt ccaggtgccg tcggggtcgc ggacgggttg gaagccgtgt tccttggtgc
     3001 ggttgtggaa cccgcagtac gcatcggagt tgtcggtgtt ggtgggtccg ccccggcagg
     3061 ccgggtcgcg gtggtcgacc tgcaggttct tgcctcgtcc gtcgcagccc ggccacaggc
     3121 agccgagtcc gccgggtcct cgcagcagtg cttggaggaa ggcggcgtcg cgggcggaac
     3181 cggtgaacag gcgttgccgg cggccgagat cgatcacgtt cccggctccg tcgaccacca
     3241 cccgccggac gtgcccgacc agcgcagcag cgaccgcctc ggctggacgg atctgggttc
     3301 cgtcgacggt gtggcagcgc cgaccatcga gcgccgcgtt cggatcgaca gtgaccggga
     3361 cgccggcgga acgggccagt tcaccttcga aggtgtcctg gtccacgacg atgttgacca
     3421 acggctcggg tgtggcaccg ccggcggccg cagcccggca gaagatccga aacatcgcat
     3481 ccgcccgccg ctgggccggg gtccggtcga ggtgttcgac gcgggcgtcg tcgccgtgga
     3541 gctctcgggc ggcggcccag tcggcgtgga gctcggcggt ctcgaaccgg tcgaagatct
     3601 cggccatcgc cgccccctgc aacgccccat ggttcgactc gagccggaac gacccatcga
     3661 tggccgattc ctgcaccgac gcgttgcgtc gttcgtggga tcgttcggcg gcctggtcgg
     3721 cgccgtcggc gtcggcgagg gcttcccact ggcgcagcgc agcacagaag tcgtcgtagc
     3781 cgagcacggt ggcgtggtgg gcgaagatcg gatccgcctc ggggacccat tcggcgaccc
     3841 gcgggttcga gatggtgcgg gccatggccc gcacgtgctc ggtcgggatc tcaccccgcg
     3901 cgtaggccgc ttcgaccaat ggcaggcggg acagggcccg gctggtctgg acccgcccat
     3961 gggcatcaga ccccgacaac cgaccgatcg ccttcaacgc ccccttggcg gtcttgtgcc
     4021 cgtcgacgct gaacgccgcg ctggtgtcga ggtgggcgat ggtcaggtcg gtgccgtgat
     4081 ccagccggcg gcgtagcttc tccaaccggc ccacgagcgc gagctcctcg gccggcgaca
     4141 ggccatccag atcgacggcc tggaccatcc ccacggcagc ttccagcgcc ttcaacgcga
     4201 ggtcggggtc gacgccgggt tccaacagat cggtcacggc aacgcctccc ccgagacatc
     4261 accggtgtgt ggtcggtggc agcgcatccg cgcagctcct tcctcatcga atgtcgacgg
     4321 tcggcgcgaa gccgacgagt ggcacctcca accccagccg gagcgcctac ccaccattca
     4381 actcaggggg tacgacaaga acgtcttgac ccacccggtg ggaggatggg gcggtgaccc
     4441 ggtggaggtg tcccgactgc ggccgcgagt tcggccgcac ccgccagggg cacgagtgcg
     4501 cgccggcgct gtcgctcgag gagtacttct cgaccgggcc cgagcgggag cg
//
LOCUS       DATISU010000213         9274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_522724, whole genome shotgun sequence.
ACCESSION   DATISU010000213 DATISU010000000
VERSION     DATISU010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9274
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522724"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="VLR27_08885"
     CDS             complement(<1..118)
                     /locus_tag="VLR27_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor"
                     /protein_id="HSP03601.1"
                     /translation="MPQPPSLTPEQRQAALAKAAEARRARAELKERLKMGSLL"
     gene            complement(154..861)
                     /gene="pyrF"
                     /locus_tag="VLR27_08890"
     CDS             complement(154..861)
                     /gene="pyrF"
                     /locus_tag="VLR27_08890"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HSP03602.1"
                     /translation="MTQIQIEAPEEVRARLCLALDTDDLVEAGRRARELGPWFGTLKV
                     GLELFASAGPAAVSALQGLGLDVFVDLKLHDIPTTVHKAARVLGSLGVAHVTFHAHGG
                     VDMLRAGVDGLREGADSAGLPAPRALAVTVLTSDDSAPPHILPMRVEAAAEAGCDGLV
                     CAVADVAEVRAIVPDLFVATTGIRTTGDDPHDQRRTATPSEALAAGVDLLVLGRTITH
                     AEDPAGAAAAVVDDALS"
     gene            941..2260
                     /locus_tag="VLR27_08895"
     CDS             941..2260
                     /locus_tag="VLR27_08895"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HSP03603.1"
                     /translation="MDVRLASLSERLRNSLFFIPMAYVVAAVLLGALAVELDQRVVAG
                     TAELPFGLSSTVDSARAILGTIASATITFAGIAFSISLLVIQLASSQYSPRVVHGLLR
                     DPFTKQIMGVVIGTFTYCLVVLRAVRGPLEDDGTPVIPSISVGFALLFGVVSILAIVA
                     FINHSAHSMDVSRILARASEDGIAVAKDLWGDDPKTGSDPEQLPEAPPDHLLVTHGSD
                     GWVRDIDFTSLAASAPPGGTVRVETEVGRYAIAGTPLCRIWPQPDDEDEACRRARKAI
                     ITGDSRTVEQDVGYAVRQLADVALRALSPGINDPTTAQDSIFHLGSVLRELLSRHPPR
                     LATPMDEDRLLLRPELLGHRELVAVAFDEVRLAAVGMPTVCIYLLEVLHLLEEAVDDD
                     GPVRDALRRQAALIRAGVDHADLGAHDADRVRDAHDRRFGPPAERIS"
     gene            complement(2331..3263)
                     /locus_tag="VLR27_08900"
     CDS             complement(2331..3263)
                     /locus_tag="VLR27_08900"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HSP03604.1"
                     /translation="MVDLTTTIGSVTLPNPVMTASGTAGHGTELGAYVDLSSLGAVVV
                     KSLAAYPWDGNPALRTHGTHGGMINSVGLQGPGVEAWRREQLPKLVDAGARVVASIWG
                     RSVDDYRRAAEQLAGCPREVVAVEINLSCPNTEAGGELLAHDPDASAATVEAVAAAGL
                     PRWAKLSPNTSGVAEVAAAVQAAGAEAVTLVNTLLGMAIDPVTGAYRLGSGERGGGVS
                     GPAIHPVAVRIVHDVHRALPDLPIVGVGGIASGEDAVEMLRAGAGAVQVGTATFADPR
                     APARVLAELTALAERIGVPRTADLTGAVHPPTPR"
     gene            complement(3265..6570)
                     /gene="carB"
                     /locus_tag="VLR27_08905"
     CDS             complement(3265..6570)
                     /gene="carB"
                     /locus_tag="VLR27_08905"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HSP03605.1"
                     /translation="MPKRTDIESIMLIGSGPIVIGQACEFDYSGTQACKVLREEGYRV
                     ILQNSNPATIMTDPDFADATYVEPLDLEHATRIIEQERPDAVLPTLGGQTGLNLAMEL
                     FEAGIIGVPGTPEMIGANAEAIATAEDREKFKQAMAGIGLGAPESATAHTLEEAELVV
                     QSIGLPVVIRPAYILGGKGTGIASTPEEFRRMAKFGLDSSPISEILIETSIKGWKEYE
                     LEVMRDRADNCVIICSIENLDPMGVHTGDSITVAPAQTLSDIEYQLMRDAAFACIRRV
                     GVETGGSNVQFAINPEDGEMVIIEMNPRVSRSSALASKATGFPIAKMAAKLAVGYTLD
                     EIPNDITKATPASFEPVIDYVVTKVPRWAFEKFPGQGDVLGTSMQSVGEAMAIGRTFP
                     ESMQKAMRSLEQGRMGLNCDPGETQMDDLSDAELLAQACIGTPDRPYQLEACLRRGMS
                     VEQVAEATKVDPWFLDQISRIVEERAHLAEVGFEAITTRELRRAKRLGFGDAQLGYLW
                     GVPESEVRARRIADGLVPTYKTVDTCAAEFEATTPYHYSTWEDTDEVVDTGKPRVIIL
                     GSGPNRIGQGIEFDYCCVHASFALRDAGYDTVMVNCNPETVSTDYDTSTRLYFEPLTT
                     EDALNVVEAEMASGNLVGVIVGLGGQTPLKLAGVLPEGTVLGTSPASIDLAEDRDLWN
                     QLCDRLDIPQPAGGTATDLDGARAIVDRIGYPALLRPSYVLGGRAMEIVYDDEGLVRA
                     MAELAGFGSLGKEGGLSAERPVLVDRFLEEATEVDVDAIRDRTGEIVIGGVMEHVEEA
                     GVHSGDSACSIPPHSLSDETVEVIEDYTRRLADALDVVGLLNVQFAVRQGQVFVIEAN
                     PRASRTVPFVAKATGVPLVKCAARVMVGATLAELRDEGLLTTPVIPNRRGQGRHVAVK
                     EAVLPFNRFPDADQVLGPEMRSTGEVMGIDRTFGLAFAKSQVAAGERLPDTGAVFFSL
                     ADRDKAQGAVAARGFVDMGFQIVATDGTADHFEAAGIPVTTRVAKLGQHAEGTGPTAV
                     DLLEDGKIDLVVNCPRGRGPRADGDYIRSAAATHRVPLLTTAAGAVAAAEGIRDRAAH
                     AGRVMSLQDYHAR"
     gene            complement(6570..7673)
                     /gene="carA"
                     /locus_tag="VLR27_08910"
     CDS             complement(6570..7673)
                     /gene="carA"
                     /locus_tag="VLR27_08910"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HSP03606.1"
                     /translation="MSAHERIRDAALVLADGSTFEGEWFGAEPAGGVATGEVVFNTAL
                     TGYQEIITDPSYAGQIVTFTYPHIGNYGVAAEDDESRRPFCRGVIVRDLARRRSSWRS
                     DGELPAFLERHGVPGIGGIDTRRLTRIIRDTGAIPGAFGPLDLVDEATLKAAAAAEPG
                     THGVDLVAQVTTPERYTYGDGPLRVAAYDFGIKVSILEQLGEFATVEVFPASASADEL
                     RAVDPDGIFLSNGPGDPEVVDYAAAEIRRLLEDDRPLFGICLGHQVLGLAAGARTVKL
                     PFGHHGGNHPVRDVTSGRIDITSQNHNFAVDPDSLGGIAEMTHVNLNDGVCEGMRLLD
                     ANAWSVQHHPEAGPGPHDARHLFEDFKHKMEQQ"
     gene            complement(7670..8953)
                     /locus_tag="VLR27_08915"
     CDS             complement(7670..8953)
                     /locus_tag="VLR27_08915"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HSP03607.1"
                     /translation="MPEPTLVIKGGRIVDATGERSGDVVVGDDGSILAVGPDLDAPQV
                     LDAGGCVVSPGFVDVHTHLREPGKEEAETVESGARGAAAGGYTCILAMPNTTPAIDSA
                     AVVREVLELGRDAPCDVRTSGAITVGRAGEQLAPIAEMAKLGVRFFTDDGTGVQDTRL
                     MRRAMEYASALDVVLAQHCEDDAMAGGGHMHEGEWSSRLGIPGQPAEAEELMVMRDIA
                     LARLTGARVHFQHLSTEGSVAMVRAAKAGGMDLTCEATTHHFTLTDECCATYDAVFKV
                     NPPLRTAADVAAVRAGLADGTIDCIATDHAPHTPDAKEQPFDEAPPGMLGLETALGLA
                     LTELDLDLQDVVALLSWRPAQLLGVEDRHGGPIIEGRRANLCVFDPDEEWTVDADTMH
                     SRSRNTPYAGRRLRGRVRHTLLEGEPVFRNGEATR"
     gene            complement(8954..>9274)
                     /locus_tag="VLR27_08920"
     CDS             complement(8954..>9274)
                     /locus_tag="VLR27_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03608.1"
                     /translation="TVDAVYLLRMQRERMTEALLPSLREYTATYGLTRDRARLLQDDA
                     LVMHPGPINRGVEVDPDVADEVRSVIIDQVRNGVSVRMAVLFLLLGSGSELLPPEVAV
                     PEAV"
BASE COUNT         1195 a         3237 c         3483 g         1359 t
ORIGIN      
        1 gcaggagcga gcccatcttc aggcgttcct tcagctcggc tcgggcccgg cgggcctcgg
       61 cggccttggc cagcgccgcc tggcgctgct ccggggtgag agaggggggc tggggcatgg
      121 cgcgacccta gacgacaccc ctcccccggc cgatcacgac agggcgtcgt ccacgaccgc
      181 tgcggcggcg ccggccgggt cctcggcatg cgtgatcgtg cgccccagga ccagcagatc
      241 gacgccggcg gcgagcgcct cgctcggcgt ggcggtgcgg cgctggtcgt ggggatcgtc
      301 gccggtggtg cggatgccgg tggtggccac gaacagatcc ggcacgatcg ctcgcacctc
      361 ggccacgtcg gccaccgcgc acacgaggcc gtcgcagccc gcctccgctg cggcctcgac
      421 ccgcatgggc aggatgtggg gcggggcgct gtcgtccgag gtcagcaccg tcacggccag
      481 ggcgcgcggt gccgggagcc cggccgagtc ggcgccctcg cggagcccgt cgaccccggc
      541 tcgcagcatg tcgacgccgc cgtgggcgtg gaaggtcacg tgggcgacgc ccagcgagcc
      601 cagcacccgc gccgccttgt gcaccgtggt cgggatgtcg tggagcttca ggtcgacgaa
      661 gacgtcgagc ccgagtccct gcagggcgct gaccgcagcg gggccggcgc tggcgaacag
      721 ctcgaggccc accttgaggg tcccgaacca cgggccgagc tcccgcgccc gccgaccggc
      781 ctcgaccagg tcgtcggtgt cgagcgccag gcacagccgc gcccggacct cctcaggtgc
      841 ctcgatctgg atctgcgtca acgcgtgccc tccctcgtct cccaggccag cctgccaccc
      901 ggtcgcgacc tcgggggtga ccaggacgcc atgatgggac atggacgttc ggctcgcctc
      961 gctgtcggaa cggctccgga acagcctgtt cttcatcccg atggcctacg tcgtggccgc
     1021 cgtgctgctc ggggccctgg ccgtggagct ggaccagcgg gtggtggcgg gcaccgccga
     1081 gctgcccttc ggcctgtcgt ccacggtcga cagcgcacgc gccatcctcg gcacgatcgc
     1141 gtcggcgacg atcaccttcg ccggcatcgc cttctcgatc tccctgctgg tgatccagct
     1201 ggcgtcgagc cagtactcgc cacgcgtcgt gcacggcctg ctgcgcgacc ccttcaccaa
     1261 gcagatcatg ggcgtcgtca tcggcacgtt cacgtactgc ctggtggtgc tgcgggcggt
     1321 gcgcggcccc ctcgaggacg acgggacccc cgtcatcccc agcatctcgg tcggcttcgc
     1381 cctgctcttc ggcgtcgtct cgatcctggc catcgtcgcc ttcatcaacc acagcgccca
     1441 ctccatggac gtgtcccgga tcctcgcccg ggcctcggag gacggcatcg ccgtggccaa
     1501 ggacctgtgg ggcgacgacc ccaagacggg cagcgatccc gagcagctgc ccgaggcgcc
     1561 ccccgaccac ctcctcgtca cccacggctc cgacggctgg gtgcgcgaca tcgacttcac
     1621 gagcctcgcc gccagcgccc cgcccggcgg caccgtgcgc gtcgagaccg aggtggggcg
     1681 ctacgccatc gccgggaccc cgctgtgccg catctggccg cagcccgacg acgaggacga
     1741 ggcctgccgg cgggcccgga aggcgatcat caccggcgac agccgcaccg tggagcagga
     1801 cgtcggctac gccgtgcgcc agctcgccga cgtggcgctg cgggccctgt cccccgggat
     1861 caacgacccc accaccgccc aggactcgat attccacctc ggcagcgtgc tgcgcgagct
     1921 gctgtcccgg caccctcctc gcctcgccac cccgatggac gaggaccgcc tgctgctgcg
     1981 gcccgagctg ctggggcacc gcgagctcgt cgccgtcgcc ttcgacgagg tccgcctggc
     2041 cgccgtcggg atgccgacgg tgtgcatcta cctgctcgag gtgctgcacc tgctggagga
     2101 ggccgtggac gacgatgggc cggttcgcga tgccctccgc cgccaggccg cgctcatccg
     2161 agccggcgtc gaccacgccg acctcggcgc ccacgacgcc gaccgcgtcc gcgacgccca
     2221 cgaccgccgg ttcggcccgc ccgccgagcg catctcctga acccgttctg gcctacatcc
     2281 gtgccagctg gtggtcacag atgtaggcca gaaccgatca ttgcccgggg tcagcggggg
     2341 gtgggtgggt ggaccgcgcc ggtgaggtcg gcggtccggg gcacaccgat ccgctcggcg
     2401 agcgcggtca gctcggcgag gacccgggct ggggcccggg ggtcggcgaa ggtggccgtg
     2461 ccgacctgga cggcgcccgc accggcccgg agcatctcga ccgcgtcctc gcccgaggcg
     2521 atgccgccga cgccgacgat gggcaggtcc ggcagggccc ggtgcacgtc gtggacgatg
     2581 cggaccgcca ccgggtggat ggccgggccg gacacgcccc cgccgcgctc ccccgagccg
     2641 agccggtagg cgccggtgac ggggtcgatg gccatcccga gcagggtgtt cacgagcgtg
     2701 acggcctcgg cgccggcggc ctgcacggcg gcggcgacct cggccacccc gctggtgttg
     2761 gggctgagct tggcccaccg gggcagcccg gctgcggcca cggcttccac ggtggcggcg
     2821 ctcgcgtcgg gatcgtgggc gaggagctcg cccccggctt cggtgttcgg gcacgacagg
     2881 ttgatctcca ccgcgacgac ctcgcgcggg cagccggcga gctgctcggc ggcgcgtcgg
     2941 tagtcgtcga cggagcggcc ccagatggac gccacgaccc gggcgccggc gtcgaccagc
     3001 ttcggcagct gctcgcgccg ccacgcctcg acgccgggcc cctggaggcc cacggagttg
     3061 atcatcccgc cgtgggtgcc gtgggtgcgc agcgccgggt tcccgtccca cgggtaggcg
     3121 gccagggact tcaccacgac cgcgccgagc gaggacaggt cgacgtaggc gccgagctcg
     3181 gtgccgtggc cggcggtgcc ggacgcggtc atgaccgggt tcggcagggt caccgacccg
     3241 atggtcgtcg tcaggtcgac cacgtcagcg ggcgtggtag tcctgcaggg acatgacccg
     3301 gccggcgtgg gcggcccggt cgcggatgcc ctcggcggcg gccacggcgc cggcggcggt
     3361 ggtcagcagc ggcacccggt gggtggcggc ggccgagcgg atgtagtcgc cgtcggctcg
     3421 gggacctcgg cctcgggggc agttcacgac caggtcgatc ttgccgtcct cgagcaggtc
     3481 cacggccgtc ggcccggtgc cctcggcgtg ctggccgagc ttggccaccc gcgtggtcac
     3541 cgggatgccg gcggcctcga agtgatcggc ggtgccgtcg gtggccacga tctggaagcc
     3601 catgtcgacg aagccgcgag cggccacggc gccctgggcc ttgtcccgat cggcgagcga
     3661 gaagaacacg gccccggtgt cggggagccg ctcccccgcc gcgacctggg acttggcgaa
     3721 ggccagcccg aaggtgcggt cgatgcccat gacctcgccg gtggagcgca tctccgggcc
     3781 gagcacctgg tcggcgtcgg ggaaccggtt gaagggcagc accgcctcct tgacggcgac
     3841 gtggcggccc tgcccccggc ggttggggat gacgggggtg gtgagcaggc cctcgtcgcg
     3901 cagctcggcg agggtggcgc cgaccatgac ccgggcggcg cacttcacca gcggcacgcc
     3961 ggtggccttg gccacgaacg gcacggtgcg gctggcccgg gggttggcct cgatcacgaa
     4021 cacctggccc tggcggacgg cgaactgcac gttgagcagg ccgaccacgt cgagggcgtc
     4081 ggcgaggcgg cgggtgtagt cctcgatgac ctcgacggtc tcgtccgaca gcgagtgcgg
     4141 cgggatcgag caggccgagt cgcccgagtg caccccggcc tcctcgacgt gctccatgac
     4201 gccgccgatg acgatctcgc cggtgcggtc ccggatggcg tcgacgtcga cctcggtggc
     4261 ctcctcgagg aagcggtcga cgaggacggg acgctcggcc gacaggccgc cctccttgcc
     4321 gagggagccg aagccggcca gctcggccat ggcgcgcacc agaccctcgt cgtcgtagac
     4381 gatctccatc gcccgaccgc ccagcacgta gctggggcgg agcagcgccg ggtagccgat
     4441 ccggtcgacg atggcgcgag cgccgtcgag gtcggtggcc gtgccgccgg ccggctgggg
     4501 gatgtccagc cggtcgcaga gctggttcca caggtcgcgg tcctcggcca ggtcgatcga
     4561 ggccgggctc gtgccgagca cggtgccctc cggcaggacg ccggcgagct tgagcggggt
     4621 ctggccgccg aggccgacga tgacaccgac caggttgccg gaggccatct cggcctcgac
     4681 cacgttcagg gcgtcctcgg tggtgagggg ctcgaagtac agccgggtcg aggtgtcgta
     4741 gtcggtggac accgtctcgg ggttgcagtt caccatcacg gtgtcgtagc cggcgtcgcg
     4801 cagggcgaag ctggcgtgca cgcagcagta gtcgaactcg atgccctggc cgatgcggtt
     4861 gggaccggag ccgaggatga tcacccgggg cttgcccgtg tcgaccacct cgtcggtgtc
     4921 ctcccaggtc gagtagtggt acggcgtcgt ggcctcgaac tccgccgcgc acgtgtcgac
     4981 cgtcttgtag gtcggcacga ggccgtcggc gatccggcgg gcccggacct cggactccgg
     5041 cacgccccag aggtagccga gctgggcgtc gccgaagccg aggcgcttgg cccggcgcag
     5101 ctcgcgggtg gtgatggcct cgaacccgac ctcggccagg tgagcgcgct cctccacgat
     5161 gcgggagatc tggtcgagga accacgggtc gaccttggtc gcctcggcga cctgctcgac
     5221 gctcatcccg cggcgcaggc aggcctcgag ctggtacggg cggtccgggg tgccgatgca
     5281 ggcctgggcc aggagctcgg cgtcggacag gtcgtccatc tgggtctcgc ccgggtcgca
     5341 gttcagcccc atgcggccct gctccagcga gcgcatcgcc ttctgcatgg actcggggaa
     5401 ggtgcggccg atggccatgg cctcgcccac cgactgcatc gacgtgccga gcacgtcgcc
     5461 ctggccgggg aacttctcga aggcccaccg gggcaccttg gtcacgacgt agtcgatgac
     5521 cggctcgaag ctggccggcg tggccttggt gatgtcgttg gggatctcgt cgagcgtgta
     5581 gccgacggcg agcttggccg ccatcttggc gatcgggaac ccggtggcct tcgaggccag
     5641 cgcgctcgag cgggacacgc gcgggttcat ctcgatgatc accatctccc cgtcctcggg
     5701 gttgatggcg aactgcacgt tcgatccgcc ggtctccacg ccgacccgcc ggatgcaggc
     5761 gaaggcggcg tcgcgcatca gctggtactc gatgtcggac agcgtctggg ccggcgccac
     5821 cgtgatcgag tcgccggtgt ggacgcccat cgggtccagg ttctcgatcg agcagatgat
     5881 cacgcagttg tccgcgcggt cgcgcatgac ctcgagctcg tactccttcc agcccttgat
     5941 ggaggtctcg atgaggatct cgctgatggg gctcgagtcg aggccgaact tggccatccg
     6001 ccggaactcc tcgggcgtgg acgcgatacc ggtgcccttg ccgccgagga tgtacgcggg
     6061 ccggatcacc acgggcaggc cgatggactg cacgacgagc tcggcctcct cgagggtgtg
     6121 ggcggtcgcc gactcgggtg cgccgaggcc gatgccggcc atggcctgct tgaacttctc
     6181 acggtcctcg gcggtggcga tggcctcggc gttggcgccg atcatctccg gcgtgccggg
     6241 cacgccgatg atgccggcct cgaacagctc catggccagg ttcagaccgg tctggccgcc
     6301 gagcgtgggc aggaccgcgt cggggcgctc ctgctcgatg atgcgggtcg cgtgctcgag
     6361 gtcgaggggc tcgacgtagg tggcatccgc gaagtccggg tccgtcatga tcgtggccgg
     6421 gttcgagttc tgcaggatca cccggtagcc ctcctcgcgg aggaccttgc aggcctgggt
     6481 gccggagtag tcgaactcgc aggcctggcc gatgacgatc gggccggagc cgatcagcat
     6541 gatcgactcg atgtcggtgc gcttgggcac tactgctgct ccatcttgtg cttgaagtcc
     6601 tcgaagaggt ggcgggcgtc gtgggggccg ggaccggcct ccgggtggtg ctggaccgac
     6661 caggcgttgg cgtcgagcag gcgcatgccc tcgcagaccc cgtcgttgag gttcacatgc
     6721 gtcatctcgg cgatgccacc gaggctgtcg gggtcgaccg cgaagttgtg gttctggctg
     6781 gtgatgtcga tgcggccgga ggtgacgtcg cgcaccgggt ggttgccgcc gtggtggccg
     6841 aacggcagct tcacggtccg ggcgccggcg gcgaggccga ggacctggtg gccgaggcag
     6901 atgccgaaca gcggccggtc gtcctccagc agccgccgga tctccgctgc ggcgtagtcg
     6961 acgacctcgg ggtcgccggg gccgttggag aggaagatcc cgtcggggtc gaccgcgcgc
     7021 agctcgtcag cgctggccga ggccgggaag acctcgacgg tggcgaactc gccgagctgc
     7081 tcgaggatcg agaccttgat gccgaagtcg taggcggcga cccggagcgg accgtcaccg
     7141 taggtgtacc gctcgggcgt ggtgacctgg gcgaccaggt cgacgccgtg ggtgccgggc
     7201 tcggctgccg cggcggcctt cagcgtcgcc tcgtcgacga ggtcgagcgg accgaacgcg
     7261 ccggggatgg cgcccgtgtc gcggatgatg cgggtgaggc ggcgggtgtc gatgccgccg
     7321 atgcccggca cgccgtggcg ctcgaggaag gcgggcagct ccccgtcgct gcgccagctg
     7381 ctgcgccggc gggcgaggtc gcggacgatg acgccccggc agaacggccg gcggctctcg
     7441 tcgtcctcgg cggccacgcc gtagttgccg atgtgggggt aggtgaaggt gacgatctgg
     7501 ccggcgtagg aggggtcggt gatgatctcc tggtagccgg tgagggcggt gttgaacacg
     7561 acctcgccgg tggccacgcc gccggcgggc tcggcgccga accactcgcc ctcgaaggtg
     7621 gagccgtcgg ccaggaccag tgcggcgtcc ctgatccgct catgggcgct catcgggtcg
     7681 cctccccgtt ccggaacacc ggttcgccct cgagcagggt gtggcgcacc cgcccgcgca
     7741 ggcgtcggcc cgcgtagggg gtgtttcggc tgcggctgtg catcgtgtcg gcgtccaccg
     7801 tccactcctc gtctgggtcg aacacgcaga ggttcgctct gcgaccctcg atgatggggc
     7861 ctccgtggcg atcctccacc ccgagcagct gtgccggtcg ccacgacagc agggccacga
     7921 cgtcctggag gtccaggtcg agctcggtga gggcgaggcc gagcgccgtc tcgaggccga
     7981 gcatcccggg aggcgcctcg tcgaagggct gctccttggc gtcgggcgtg tggggggcgt
     8041 ggtcggtggc gatgcagtcg atcgtgccgt cggccaggcc ggcccggacc gccgccacgt
     8101 cggcggccgt gcgcagcggc gggttgacct tgaacacggc atcgtaggtg gcgcagcact
     8161 cgtccgtgag cgtgaagtgg tgcgtggtgg cctcgcaggt caggtccatg ccgcccgcct
     8221 tggcggcccg caccatggcc accgatccct cggtggagag gtgctggaag tgcacccggg
     8281 cgccggtgag tcgggcgagg gcgatgtcgc gcatcaccat gagctcctcg gcctcggcgg
     8341 gctggccggg gatgcccagg cggctcgacc actcgccctc gtgcatgtgg ccgccaccgg
     8401 ccatggcgtc gtcctcgcag tgctgggcca ggaccacgtc gagcgccgag gcgtactcca
     8461 tggcccggcg catcagccgc gtgtcctgca cgccggtgcc gtcgtcggtg aagaaccgca
     8521 cgcccagctt ggccatctcg gcgatggggg cgagctgctc gccggcccgg ccgaccgtga
     8581 tggcaccgga ggtgcgcacg tcgcacgggg cgtcgcgccc gagctcgagc acctcgcgga
     8641 cgacggcggc ggagtcgatc gccggcgtgg tgttcggcat ggccaggatg caggtgtagc
     8701 cgccggccgc tgcaccccgg gcgccggact cgaccgtctc ggcctcctcc ttgccgggct
     8761 cgcgcaggtg ggtgtggacg tcgacgaagc cggggctgac gacgcatcca ccggcgtcga
     8821 gcacctgggg ggcgtcgagg tcggggccga cggcgaggat cgatccgtcg tcgccgacga
     8881 cgacgtcgcc ggagcgctcg ccggtggcgt cgacgatgcg accgcccttg atcacgaggg
     8941 tgggttcggg catctagacg gcctccggga cggcgacttc ggggggcagg agctcggatc
     9001 cggatccgag caggaggaag aggacggcca tgcggacgga cacgccgttg cggacctggt
     9061 cgatgatcac cgagcggacc tcgtcggcca cgtcagggtc gacctcgacg ccgcggttga
     9121 tcggacccgg gtgcatcacg agcgcatcgt cctgcagcag gcgggcccgg tcgcgggtga
     9181 gcccgtaggt ggccgtgtac tcgcgcagcg agggcagcag cgcctcggtc atccgctccc
     9241 gctgcatgcg cagcaggtac accgcgtcga ccgt
//
LOCUS       DATISU010000214         4760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_708754, whole genome shotgun sequence.
ACCESSION   DATISU010000214 DATISU010000000
VERSION     DATISU010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708754"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VLR27_08925"
     CDS             complement(<1..324)
                     /locus_tag="VLR27_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSP03609.1"
                     /translation="MTPIIVAEGLERRFGDLIAVDGVDLSIEAGEVFGFLGPNGAGKS
                     TTVRMLTTLLKPTAGHARVAGFDVVRQAGDVRRAIGVALQDAAIDPLMTGSELVHLQA
                     VLHGIP"
     gene            complement(381..1304)
                     /locus_tag="VLR27_08930"
     CDS             complement(381..1304)
                     /locus_tag="VLR27_08930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03564 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HSP03610.1"
                     /translation="MRIGINGSSLLTSPDVGTITTDIVTAEAEGFTSYWLAQTGMADA
                     LGVLSAAGPATNVVELGTAVVPTWLQHPMALAASALTTQANTHGRLVLGIGLSHQPAV
                     EHRWHMTWEKPIRHMLDYLEVLVPALHGETVQHHGEVWSLTADAPMPRPTDEPPKVML
                     AALGDQMLRIAGTRTDGTILWCVGPRTVETHIAPRINEAAADADRPTPSIVCSIPCWV
                     TDDPDSARAFIAKVLAGYAELPSYRAMLDIEGIHGLEDLSFVGSEQQVRDGIARVAEA
                     GATDFTAVVMGANPDETAATRAVLAAAAAAG"
     gene            complement(1332..2870)
                     /locus_tag="VLR27_08935"
     CDS             complement(1332..2870)
                     /locus_tag="VLR27_08935"
                     /inference="COORDINATES: protein motif:HMM:NF005760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HSP03611.1"
                     /translation="MNGAELLLQTAVEQGVDVCFANPGTTEMELVGALDQVSGMRGVL
                     GLFEGVVTGAADGYGRVTGRPALTLLHLGPGFANGIANLHNARRAHTPVVNLIGDHAS
                     WHLSADAPLTSDIESLASPVSLHVERITGPGHTGSAMLEAIAAAMGPPSGVATLIVPQ
                     DAAWGAADLHDVGLPPEVEAPVDGDAVGAAASKLRSGDGVAILLGGVVRRAELEAAHR
                     IAEDTGAKVWVDTFPAVLEQGRGLPHFDAIPYFPEQAIDALAGIRTLVCAGTRAPVAF
                     FGYQSLGRSDLLDKGCDVLELCPPGTRGHLGLDALVDALECPAAKVTDPDAPEVPGGD
                     LDMITMGQVVGALQPEGAIVVNEAATSGLGWAITAGSAPPHESFSLTGGAIGQGLPSA
                     VGAAVAAPDRKVIALQADGSGLYTVQSLWTMAREGLDVTVVVCANRAYRILQMELHRT
                     GNEDPGPQARSLTDLGSPELDWVSLAKGFGVEAMQATTIEELRAGLERGLATDGPFLV
                     EALI"
     gene            complement(2899..4176)
                     /locus_tag="VLR27_08940"
     CDS             complement(2899..4176)
                     /locus_tag="VLR27_08940"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF005600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HSP03612.1"
                     /translation="MHQGRLPVTFEDVRAAHAAIAGAVVRTPMARSHTLSAITGADVW
                     VKFENLQYTASFKERGARNRLLRLEPGQSDAGVVAMSAGNHAQAVAHHATLLGIRSTI
                     VMPEGTPANKISATEDNGATVVVAGTNLDEAKEVAMQIVAEEGATFIAPYDDPAIIAG
                     QGTCGIELIEDGPDLDALLVPVGGGGLFSGICVAARTLAPRIELIGVQSELHPAMLLA
                     TGRTAAPPDLTWGPTIAEGIAVKTPGRFTTEIISALADDVVAVPERLIEEAVIWFLEI
                     EKMVSEGAGAAPLAALLDDPDRFAGRTVGLVLSGANIDLRLLSAVIMRGLARTGRLCR
                     VVIEVPDSPGALGELTAAVGRAGGNIVDLWHHREFGVHSARVTEIELLVETRDGESAE
                     QLKESLSGQGYIVDDVPRRAAVRTPSPDPRAGP"
     gene            4236..>4760
                     /locus_tag="VLR27_08945"
     CDS             4236..>4760
                     /locus_tag="VLR27_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HSP03613.1"
                     /translation="MAEYTTPEWHPAFEEYCEAIWELAEDDIAVIQARIAERLDVSRP
                     AVSEMIRKLADEGLVATDGGKITLTDAGQRLAERVVRRHRLAERFITDILGLSWAVAH
                     QEAGRWEHVMSTEVEEAMDAVLGSPTTCPHGNPIPGSRYAEPDTTPLATVGVGSTFVV
                     DRIPEELEFTPGLLD"
BASE COUNT          639 a         1652 c         1811 g          658 t
ORIGIN      
        1 cgggatgccg tgcagcactg cctgcaggtg caccagctcc gagccggtca tcagcgggtc
       61 gatggctgcg tcctgcagcg cgacgccgat ggcgcggcgc acgtcgccgg cctggcggac
      121 gacgtcgaag ccggcgacgc gggcgtgccc ggccgtcggc ttcagcaggg tggtgagcat
      181 gcggacggtg gtggacttgc cggcgccgtt cgggccgagg aagccgaaca cctcgcccgc
      241 ctcgatggag aggtccacgc cgtcgaccgc gatgaggtca ccgaagcgcc gctcgagccc
      301 ctcggccacg atgatgggtg tcacgagcgg gtcaccgtag acctgcaggc ccgtggggga
      361 tcacgatggg caccccgcgc tcagccggcg gcagcagcgg cggccagcac ggcccgggtg
      421 gccgcggtct cgtcggggtt cgcgcccatc accaccgcgg tgaagtcggt ggccccggcc
      481 tcggcgaccc gggcgatgcc gtcgcgcacc tgctgctcgc tgcccacgaa ggacaggtcc
      541 tcgaggccgt ggatgccctc gatgtcgagc atcgcccggt acgacggcag ctcggcgtag
      601 ccggccagca ccttggcgat gaaggcccgg gccgagtcgg ggtcgtcggt gacccagcac
      661 gggatgctgc acacgatcga cggcgtgggc cggtcggcat cggcggccgc ctcgttgatg
      721 cgcggggcga tgtgggtctc gaccgtgcgg ggcccgacgc accacaggat cgtcccgtcg
      781 gtgcgggtgc cggcgatgcg cagcatctgg tcgccgagcg cggcgagcat gaccttgggc
      841 ggctcgtccg tggggcgcgg catgggcgca tcggcggtga ggctccacac ctcgccgtgg
      901 tgctgcacgg tctcgccgtg gagggccggc acgaggacct cgaggtagtc gagcatgtgg
      961 cggatcggct tctcccacgt catgtgccag cggtgctcga ccgctggctg gtgggacagg
     1021 ccgatgccga gcacgagccg cccgtgggtg ttggcctgcg tcgtcagggc gctggcggcg
     1081 agcgccatgg ggtgctggag ccacgtgggc acgaccgcgg tgccgagctc gacgacgttg
     1141 gtggccgggc cggcggcgga cagcacgccg agcgcgtcgg ccatgccggt ctgggccagc
     1201 cagtaggagg tgaacccctc ggcctcggcc gtgacgatgt cggtggtgat ggtgcccacg
     1261 tcggggctgg tgaggagcga ggagccgttg atgccgatgc gcatcccggc gaccctacga
     1321 cccgactcgg actagatcag cgcttcgacc aggaacgggc cgtcggtggc cagcccgcgc
     1381 tcgaggccgg cgcgcagctc ctcgatggtg gtggcctgca tcgcctcgac gccgaagccc
     1441 ttcgccagcg acacccagtc gagctcgggc gacccgaggt cggtgagcga gcgggcctgg
     1501 gggccggggt cctcgttgcc ggtgcggtgc agctccatct gcaggatccg gtacgcccgg
     1561 ttggcgcaga cgacgaccgt cacgtcgagg ccttcgcgag ccatggtcca cagcgactgg
     1621 acggtgtaga ggccggagcc gtcggcctgg agggcgatca ccttgcggtc gggcgcggcg
     1681 acggcggcac ccacggcgct gggcagaccc tggccgatgg ccccgccggt gagggagaac
     1741 gactcgtgcg gcggcgcgct gccggcggtg atggcccagc cgagtccgga ggtggccgcc
     1801 tcgttgacga cgatggcgcc ctcgggctgc agggccccga cgacctgacc catcgtgatc
     1861 atgtcgaggt cgccgccggg cacctcgggc gcgtccgggt ccgtgacctt ggcggccggg
     1921 cactcgagcg catcgacgag ggcgtcgagg ccgaggtgcc cgcgggtgcc gggtgggcac
     1981 agctcgagca cgtcgcagcc cttgtcgagc aggtcgctgc ggccgagcga ctggtagccg
     2041 aagaaggcga cgggcgcccg ggtgccggcg cacacgaggg tgcggatgcc ggcgagcgcg
     2101 tcgatggcct gctcggggaa gtacgggatg gcgtcgaagt ggggcaggcc gcggccctgc
     2161 tcgagcaccg cagggaacgt gtcgacccag accttggcgc cggtgtcctc ggcgatgcgg
     2221 tgcgcggcct cgagctcggc gcggcgcacg acgccaccga gcaggatcgc gacgccgtcc
     2281 ccgctgcgga gcttgctggc cgccgcaccc acggcgtcgc cgtcgactgg tgcctcgacc
     2341 tcgggcggga ggcccacgtc gtgcaggtcg gcggcgcccc acgccgcgtc ctgggggacg
     2401 atgagcgtgg ccacccccga gggcgggccc atggcggcgg cgatggcctc gagcatggcg
     2461 ctgccggtgt ggccgggccc ggtgatccgc tcgacgtgga gcgacaccgg cgatgccagc
     2521 gactcgatgt cgctcgtcag cggcgcgtcc gccgacaggt gccaggaggc gtggtcaccg
     2581 atcaggttga ccaccggggt gtgcgcccgg cgggcgttgt ggaggttggc gatgccgttg
     2641 gcgaagccgg ggccgaggtg cagcagcgtg agcgcgggac gtccggtgac ccgcccgtaa
     2701 ccgtcggccg ctcccgtgac gacgccttcg aacagcccga gcacgccgcg catgccggac
     2761 acctggtcga gcgcgccgac cagctccatc tcggtggtcc cggggttggc gaagcagacg
     2821 tcgacgcctt gctcgacggc cgtttgcagc agcagctctg caccgttcac gtggttgtcc
     2881 tcctggtcgt ggtgtggatc acggacccgc tcgtgggtcc ggcgacggcg tgcgcaccgc
     2941 agcgcggcgt ggcacgtcgt cgacgatgta gccctgcccg ctcagggact ccttcagctg
     3001 ctcggcgctc tcgccgtcgc gggtctcgac gagcagctcg atctcggtga cccgggcgga
     3061 gtgcacgccg aactcgcggt ggtgccacag gtcgacgatg ttgcccccgg cccgaccgac
     3121 cgcggcggtg agctcgccca gcgcaccggg actgtcgggc acctcgatca ccacccggca
     3181 cagacggccg gtgcgcgcca ggccgcgcat gatgacggcc gacagcaggc gcaggtcgat
     3241 gttggccccc gacagcacca gacccaccgt gcggccggcg aagcggtcgg gatcgtcgag
     3301 cagcgcggcg agcggggcgg cccccgctcc ctcgctgacc atcttctcga tctcgaggaa
     3361 ccagatcacc gcctcctcga tgaggcgctc gggcacggcc acgacgtcgt cggccagggc
     3421 ggagatgatc tcggtggtga agcgacccgg cgtcttcacg gcgatgccct cggcgatggt
     3481 gggaccccag gtgaggtccg ggggcgctgc ggtgcgaccg gtggcgagga gcatcgccgg
     3541 gtgcagctcg gactgcacgc cgatgagctc gatgcgcggc gccagcgtgc gggcagccac
     3601 gcagatcccc gagaacaggc cgccgccgcc caccgggacg agcagggcgt cgaggtcggg
     3661 cccgtcctcg atcagctcga tgccgcacgt gccctgaccg gcgatgatgg cggggtcgtc
     3721 gtagggggcg atgaacgtgg cgccctcctc ggcgacgatc tgcatcgcca cctccttggc
     3781 ctcgtcgagg ttggtgccgg cgaccacgac cgtggcgccg ttgtcctcgg tggccgagat
     3841 cttgttggcc ggcgtgccct ccggcatcac gatggtggag cggatgccga gcagggtggc
     3901 gtggtgggcc acggcctggg cgtggttgcc ggccgacatg gccacgacgc cggcatcgga
     3961 ctgccctggc tcgaggcgca ggagccggtt gcgggcaccg cgctccttga acgaagcggt
     4021 gtactgcagg ttctcgaact tcacccacac gtcggcaccg gtgatcgccg acagggtgtg
     4081 cgaccgggcc atcggcgtgc ggaccacggc accggcgatg gctgcatgcg cggcacgcac
     4141 gtcctcgaag gtcaccggca gccgtccctg atgcacggct gcatcgtgtc acggcgcggg
     4201 gtccggtgag ggcccgacgg gcctaggatc tggggatggc cgagtacacc acccccgagt
     4261 ggcatccggc gttcgaggag tactgcgagg cgatctggga gctcgccgag gacgacatcg
     4321 ccgtcatcca ggcccggatc gccgagcgcc tcgacgtctc ccggccggcg gtctcggaga
     4381 tgatccgcaa gctcgccgac gagggtctgg tggccaccga cggcggcaag atcaccctca
     4441 ccgacgccgg tcagcgcctg gccgagcgcg tcgtgcgccg ccaccggctg gccgagcgct
     4501 tcatcaccga catcctcggt ctctcatggg cggtcgccca ccaggaggcc ggccggtggg
     4561 agcacgtcat gtccaccgag gtcgaggagg ccatggacgc cgtcctcggc tcccccacca
     4621 cctgccccca cggcaacccg atccccggct cccgctacgc cgagccggac accaccccgc
     4681 tcgccaccgt gggcgtgggc tcgaccttcg tggtcgaccg catccccgag gagctcgagt
     4741 tcacgcccgg cctgctcgac
//
LOCUS       DATISU010000215         3380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_755457, whole genome shotgun sequence.
ACCESSION   DATISU010000215 DATISU010000000
VERSION     DATISU010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755457"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VLR27_08950"
     CDS             <1..214
                     /locus_tag="VLR27_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSP03614.1"
                     /translation="VSAVIGLAHALGLRTVAEGVEDEAQRDRLHELGVDIGQGYLWSR
                     AVPVAEAVEMVRGAEPTGTPCWAPVD"
     gene            complement(225..1226)
                     /locus_tag="VLR27_08955"
     CDS             complement(225..1226)
                     /locus_tag="VLR27_08955"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSP03615.1"
                     /translation="MKISRLLVALFAVLALLAGACGDDDDADVASSGDDTDEADEAAT
                     ETETEAEEETEAGDGPAIRIGAQDFGESAILAEIYGQALEANGYSVEQVSVGGFRDVL
                     LGAFDNGEVNFSPEYAASMLEFLNGNAGEATGDADETVELLQSQLESMGLVALEPAPA
                     VDTNAFVVTQETADAQGLAALSDLPADGGDLTLGAPQDCETNPVCIPGLQEVYGVDLS
                     GDFVPLDAGLVADALAAGEIQVGVLFSTSGRIADEGWVLLEDDMDMLAADNVTPVVSA
                     ELEEAYGEELIDLINEISAAIDTDTLTDLNRRFDIDKEDADDIAADFISEAGLGAEG"
     gene            complement(1265..1972)
                     /locus_tag="VLR27_08960"
     CDS             complement(1265..1972)
                     /locus_tag="VLR27_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP03616.1"
                     /translation="MIEEALAYLTDADSWWGRNGLVNLTWNHVRLSGAALLAAIVLAV
                     PPALLLGHRHRGGLLTVTLVNLSRALPTFAVIAFVLPVSYQLDLGLGFWPTFVALVLL
                     ALPPVFVNAYTGVDDVDAAVVEAARGMGMREAEVLRRVEVPAAMPLLAAGVRVSAVQV
                     VATTTLGTLVGYRCLGTPIIQGIAERDYGKVLAAAIIVAALSLLTDAAFGALGRRLTP
                     WAETPAIETKASVQHAT"
     gene            complement(1969..2643)
                     /locus_tag="VLR27_08965"
     CDS             complement(1969..2643)
                     /locus_tag="VLR27_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP03617.1"
                     /translation="MSVFATRHDRLLSWEWIGDHTDEIWERTLQHLQLTGYAVGIGLV
                     IAMGMSMVALRWRRAYAPLAAVSGALYSIPSLALFAILVPYTGLGFRPVLIALVTYTL
                     LILLRNIVAGIDGVPRSVLEAADGMGYEPWRRFLAVDLRLATPAIIGGIRVATVTVIG
                     LVTVGALVGSGGYGVFINDGLSRNFTTPIIVGGGLSIVMAIAFDLLFVAVQRLTTPWS
                     RAGTAP"
     gene            complement(2640..>3380)
                     /locus_tag="VLR27_08970"
     CDS             complement(2640..>3380)
                     /locus_tag="VLR27_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSP03618.1"
                     /translation="VEELVDLVGLERQMLDRYPSALSGGQQQRVGVARALAADPPILL
                     MDEPYSAVDPIVRARLQDELLDLQRRVHKTIVLVTHDVDEALKLGDRVVILNVGGVLE
                     QAARPAEILADPASPFVADFLGEERGLKRLALVRVRDITPDTGPVADVGDDADSVRAL
                     MQAHRVTWFGIRDQGRLLGWQWGSALDGAVQAEGAEPFRTVVSPDTTLRSALDGIVNS
                     LTNVAVVIDDDGEYRGMVRIEEISEGVR"
BASE COUNT          496 a         1129 c         1274 g          481 t
ORIGIN      
        1 cgtctcggcc gtgatcggtc tcgcccacgc actgggcctg cgcaccgtcg ccgagggcgt
       61 cgaggacgag gcccagcgtg accggttgca cgagctcggc gtcgacatcg gccaggggta
      121 cctgtggagc cgagcggtgc ctgtcgccga ggcggtggag atggtgcgtg gtgccgagcc
      181 gaccgggacc ccgtgctggg ccccggtcga ctgacggcag cggatcagcc ctcggcgccc
      241 aggccggctt cgctgatgaa gtccgccgcg atgtcgtcgg cgtcctcctt gtcgatgtcg
      301 aagcgccggt tcaggtcggt gagggtgtcg gtgtcgatgg cggcgctgat ctcgttgatc
      361 aggtcgatga gctcctcgcc gtaggcctcc tccagctcgg cggagaccac cggggtgacg
      421 ttgtcggcgg cgagcatgtc catgtcgtcc tcgagcagga cccatccctc gtcggcgatg
      481 cggccgctgg tggagaacag cacgccgacc tggatctcgc cggcggcgag cgcgtcggcc
      541 accaggccgg cgtcgagggg cacgaagtca cccgacaggt ccacgccgta cacctcctgg
      601 agcccgggga tgcagaccgg gttggtctcg cagtcctgcg gggcgccgag ggtgaggtcg
      661 ccaccgtcgg ctgggaggtc ggacagcgcc gcgaggccct gcgcgtcggc cgtctcctgg
      721 gtgacgacga aggcgttggt gtccacggcc ggcgccggtt cgagggcgac cagacccatc
      781 gactcgagct gggactggag cagctcgacg gtctcgtcgg cgtcacccgt ggcctcgccg
      841 gcgttgccgt tgaggaactc gagcatggag gcggcgtact ccggcgagaa gttcacctcg
      901 ccgttgtcga acgccccgag cagcacgtcg cgaaagccgc cgacgctgac ctgctcgacc
      961 gagtagccgt tggcctcgag cgcctggccg tagatctcgg cgaggatggc cgattcgccg
     1021 aagtcctggg cgccgatgcg gatggcgggg ccatcgccgg cctcggtctc ctcttcggcc
     1081 tcggtctcgg tctcggtggc agcttcgtcg gcttcgtcgg tgtcgtcgcc ggacgatgcg
     1141 acgtcagcgt cgtcgtcgtc gccgcacgcg ccggccagca gggccaacac ggcgaacagc
     1201 gcgacgagca gtcgggagat cttcatgggt gttcctttcg ggggtcggtc aggggaatcc
     1261 tggctcacgt ggcgtgctgg acgctcgctt tggtctcgat ggccggggtc tcggcccacg
     1321 gagtgaggcg tcggccgagg gcgccgaagg cagcgtcggt gagcagggac agcgcggcga
     1381 cgatgatcgc cgccgccagc accttgccgt agtcgcgctc cgcgatgccc tggatgatcg
     1441 gggttccgag gcagcggtag ccgaccaggg tgccgagggt ggtggtggcc acgacctgca
     1501 ctgcggacac ccgcacgcct gcggcgagca gcggcatggc ggcgggcacc tcgacccggc
     1561 gcagcacctc tgcctcgcgc atgcccatgc cccgcgccgc ttccacgacg gcagcgtcga
     1621 cgtcgtcgac gccggtgtag gcgttgacga agaccggggg cagggcgagc agcaccaggg
     1681 ccacgaacgt tggccagaac cccaggccga ggtcgagctg gtaggagacc gggaggacga
     1741 acgcgatcac ggcgaaggtc ggcagcgccc ggctgaggtt cacgagcgtg accgtcagta
     1801 gcccgccccg gtgccgatgc ccgagcagca gggcgggagg cacggcgagc acgatggcgg
     1861 cgagcagggc ggcgccggac aggcgcacgt ggttccaggt caggttgacc agcccgttgc
     1921 gcccccacca ggagtcggca tcggtcaggt acgcgagtgc ctcctcgatc atggggccgt
     1981 ccccgctcgg gaccacgggg tcgtgagccg ctgcacggcg acgaacagca ggtcgaacgc
     2041 gatggccatg acgatcgaca gcccaccgcc cacgatgatc ggcgtggtga agttccggct
     2101 gagcccgtcg ttgatgaaca cgccgtagcc gccgctgccg acgagcgcgc ccacggtcac
     2161 caggccgatg accgtgacgg tggcgacgcg gatgccgccg atgatcgccg gcgtggccag
     2221 tcggaggtcg acggcgagga agcgccgcca cggctcgtag cccatgccgt cggccgcttc
     2281 gaggacggat ctcggcacgc cgtcgatgcc ggcaacgatg ttccgcagca ggatgaggag
     2341 cgtgtaggtg acgagcgcga tgagcaccgg gcggaacccg aggccggtgt aggggacgag
     2401 gatggcgaag agcgccaggc tcgggatgct gtagagcgcg ccggacacgg cagcgagggg
     2461 ggcgtacgca cgacgccacc gcagcgcgac catcgacatc cccatggcga tgacgaggcc
     2521 gatgcccacg gcgtacccgg tcagctggag gtgctggagc gtgcgctccc agatctcgtc
     2581 cgtgtggtca ccgatccact cccatgacag cagccggtcg tggcgggtgg cgaagacgct
     2641 caccggacgc cctcgctgat ctcctcgatg cggaccatgc cccggtactc gccgtcgtcg
     2701 tcgatcacca ccgccacgtt cgtgagggag ttgacgatgc cgtcgagcgc gctgcgcagc
     2761 gtcgtgtcgg ggctgacgac cgtgcggaag ggctcggcgc cctcggcctg cacggcgccg
     2821 tcgagcgcgg agccccactg ccagccgagc aggcggccct ggtcgcggat gccgaaccac
     2881 gtcacccggt gggcctgcat gagggcgcgg acgctgtcgg cgtcgtcgcc gacgtcggcg
     2941 accgggccgg tgtcgggggt gatgtcgcgg acgcgcacca gggcgaggcg cttcagccca
     3001 cgctcctcgc cgaggaagtc ggcgacgaag ggcgacgccg ggtcggccag gatctctgca
     3061 ggtcgcgccg cctgctcgag cacgccgccg acgttgagga tgacgacccg atcgccgagc
     3121 ttcagggcct cgtcgacgtc gtgggtcacg aggacgatgg tcttgtgcac gcgccgctgc
     3181 aggtcgagca gctcgtcctg gagcctggcc cggacgatcg ggtcgacggc gctgtagggc
     3241 tcgtccatca gcaggatcgg cgggtcggcg gccagagccc gggcgacgcc gacccgctgc
     3301 tgctggccgc cggacagcgc tgacgggtag cggtcgagca tctggcgctc gagccccacg
     3361 aggtcgacca gctcctcgac
//
LOCUS       DATISU010000216         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_150661, whole genome shotgun sequence.
ACCESSION   DATISU010000216 DATISU010000000
VERSION     DATISU010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150661"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VLR27_08975"
     CDS             <1..522
                     /locus_tag="VLR27_08975"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HSP03619.1"
                     /translation="GMDRAQADYMGMLGTVMNALALQDILERKGQPTRVQTAIHMAQI
                     AEPYIRRRAIRHLEKGRVVIFGAGTGNPFFTTDTTAALRAVEIDAEAVLKGSHSGVDG
                     IYTADPRTDPDATKIDHLTYLDVLNRGLAVMDSTAITLCMDNALPILVFDLMTPGNVR
                     SILQGQAIGTLVS"
     gene            519..1088
                     /gene="frr"
                     /locus_tag="VLR27_08980"
     CDS             519..1088
                     /gene="frr"
                     /locus_tag="VLR27_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HSP03620.1"
                     /translation="MNDDLVSMILDDAATKMTAAVSRTREEFSAVRTGRASPAFVEKL
                     SVEYYGTDVPLQQLAGFAVPEARTLVITPFDKDSIPGIEKAIQQSDLGLNPSSDGVNI
                     RLNFPQLTEERRRDYVRVVKQKAEDGRVQIRNARRSARQELESLQKDGDLSEDDLVRA
                     EKELDKVTHAREAEIDKALDQKEQELLEV"
     gene            1105..>2333
                     /locus_tag="VLR27_08985"
     CDS             1105..>2333
                     /locus_tag="VLR27_08985"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HSP03621.1"
                     /translation="MNDDENQSDSGRDRPADPEGVRIIGAEEAAEALERDDIAHRRAP
                     EEPRPGDRPGSPPPDAPEPVLRFPLSSSAADEPSAELPHWTEPPTGQQSALGGTIFDD
                     DSGEEDWSGFSAAPRWRDERDEWSEDEDLRAFGGDDTRVGALDTDRPDPDDFFTFDDL
                     DAAPVGRSVFADEVEVAEADAIYDEDYAPVDYDEAGFGDAGYVEAAPSGENPALATGP
                     RKIVVGGGRVTPGNPATPRPSRGGVGGDRDIGQAAIVGVGFLVVAAVLFFLGPATAAL
                     LVVGVVTFAAAELFTKLREVGYQPAALLGIVATAGMAIGTYHRGEAAIPVVLVLVLAF
                     SMLWFLFGAGEGQATLNIGVTLLGVAWIGMFGSYGTALLGVQGGEGIGDAGKGILFGA
                     ILGTVAYDIGGYFVGRSA"
BASE COUNT          357 a          882 c          784 g          310 t
ORIGIN      
        1 ggcatggacc gcgcccaggc cgactacatg ggcatgctcg gcacggtcat gaacgcgctc
       61 gcgctccagg acatcctcga gcgcaagggc cagcccaccc gggtccagac cgccatccac
      121 atggcccaga tcgccgagcc ctacatccgc cggcgggcca tccgacacct cgagaagggc
      181 cgggtcgtga tcttcggggc gggcaccggc aacccgttct tcaccaccga caccaccgcc
      241 gcgctgcggg cggtcgagat cgacgccgag gcggtgctga agggcagcca ctccggcgtc
      301 gacggcatct acaccgccga cccgcgcacc gacccggacg cgaccaagat cgaccacctg
      361 acgtacctcg acgtgctcaa ccgtggcctg gccgtcatgg actccacggc gatcacgctg
      421 tgcatggaca acgccctgcc catcctcgtc ttcgacctca tgacgccggg caacgtgcgg
      481 tccatcttgc aggggcaggc gatcggtacg ctggtttcgt gaacgacgac ctcgtgtcga
      541 tgatcctcga cgacgctgcg acgaagatga cggccgccgt ctcgcgcacc cgcgaggagt
      601 tctcggcggt gcgcacggga cgagcgtcgc cggccttcgt ggagaagctg tcggtcgagt
      661 actacggcac cgacgtgccg ctgcagcagc tggccggctt cgccgtgccc gaggcccgca
      721 cgctcgtcat cacgcccttc gacaaggact ccatccccgg catcgagaag gccatccagc
      781 agtccgacct ggggctcaac ccgtccagcg acggcgtgaa catccggctg aacttcccgc
      841 agctcaccga ggagcgccgc cgggactacg tccgggtcgt gaagcagaag gccgaggacg
      901 gtcgcgtgca gatccgcaac gcccgccggt ccgctcgcca ggagctcgag tcgctccaga
      961 aggacggcga cctctcggag gacgacctgg tccgggccga gaaggagctc gacaaggtca
     1021 cccacgcacg ggaggccgag atcgacaagg cgctcgacca gaaggagcag gagctcctcg
     1081 aggtctgatg ggaggcccac gtccatgaat gacgacgaga accagtccga cagcggtcgg
     1141 gaccggccgg ccgatccgga gggcgtgcgc atcatcgggg ccgaagaggc cgccgaagcg
     1201 ctcgagcgcg acgacatcgc ccaccggcgc gccccggagg agccccgtcc gggcgatcgt
     1261 cccggctcac cgcccccgga cgcacccgag ccggtgctgc gcttcccgct gtcgtcgtcg
     1321 gccgccgacg agccctcggc cgagctgccg cactggaccg agccccccac cgggcagcag
     1381 tccgccctcg gcggcacgat cttcgacgac gactccggcg aggaggactg gtccggcttc
     1441 tcggcggcac cccgctggcg cgacgagcgc gacgagtgga gcgaggacga ggacctgcgg
     1501 gccttcggcg gcgacgacac ccgcgtcggc gccctcgaca ccgaccggcc cgatcccgac
     1561 gacttcttca ccttcgacga cctcgacgcc gcgcccgtcg ggcggtcggt gttcgccgac
     1621 gaggtcgagg tggccgaggc cgacgccatc tacgacgagg actacgcgcc ggtcgactac
     1681 gacgaggccg gcttcggcga cgccggctac gtggaggccg cgccgagcgg cgagaacccg
     1741 gcactggcca ccggaccccg caagatcgtc gtcggcggcg gacgggtcac tcccggcaac
     1801 cccgccaccc cccgcccgtc ccgcggcggc gtgggcggcg accgcgacat cggccaggcc
     1861 gccatcgtcg gcgtcggctt cctcgtcgtg gccgccgtgc tgttcttcct cggcccggcc
     1921 accgccgcgc tgctcgtggt cggcgtggtc accttcgccg ccgccgagct gttcacgaag
     1981 ctccgcgagg tcggctacca gcccgctgcg ctgctcggca tcgtcgccac cgccggcatg
     2041 gccatcggca cctaccaccg gggcgaggcc gccatccccg tggtgctcgt gctcgtgctc
     2101 gccttctcca tgctctggtt cctgttcggg gccggcgaag ggcaggccac gctgaacatc
     2161 ggcgtcacgc tgctcggtgt cgcgtggatc ggcatgttcg gctcctacgg caccgcgctg
     2221 ctcggcgtgc agggcggcga gggcatcggc gacgccggca agggcatcct cttcggcgcc
     2281 atcctcggca ccgtcgcgta cgacatcggc ggctacttcg tcggtcgcag cgc
//
LOCUS       DATISU010000217         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_523197, whole genome shotgun sequence.
ACCESSION   DATISU010000217 DATISU010000000
VERSION     DATISU010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523197"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VLR27_08990"
     CDS             <1..519
                     /locus_tag="VLR27_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03622.1"
                     /translation="TTVADGLRRDGHEIVDLCLPDPASPLTCRGLVEHRCPLDEGADL
                     AITALQHPQDAPSAGTVCARRDGIPVVALGRHDVSHPDLGSALARVAELGDDHVTATV
                     QVALQQVLTEMGIEGGDVVLHRDAVCEQIVATVPGPLDDPTRSRIAVRLLDAATASGR
                     HGRRRDVAVRCA"
     gene            complement(527..1189)
                     /locus_tag="VLR27_08995"
     CDS             complement(527..1189)
                     /locus_tag="VLR27_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP03623.1"
                     /translation="MSTIPIRVFVVDDHEVVRRGLMDLLDAVDDIEIAGEAGTVEDAI
                     GRIPGSNVDVAVLDVRLPDGTGIDIARAVRERDSTIQCLMLTSFDDEDALLNAILAGA
                     SGYLLKQVKGLDLIDAVRRVAAGQSLLDPAVTERVLRAVRNREQRDERLERLTPQEHR
                     IVELLAEGRTNREIGADMFLAEKTVKNYVSNVLAKLGMSQRTEAAVWAARLDERGELH
                     RD"
     gene            1300..1686
                     /locus_tag="VLR27_09000"
     CDS             1300..1686
                     /locus_tag="VLR27_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSP03624.1"
                     /translation="MSEVWTGFQTMTADECRRLLATVDIGRVAISAGALPFVLPVQYA
                     LQDDHLLMRTPGHHEVGDGIDGQVVGFEADTIDLDHGVGWCVSVTGTVRVVPDAPMAD
                     PVHRWFSDGVVLALSTDVIVGHRVAV"
     gene            complement(1700..>2170)
                     /locus_tag="VLR27_09005"
     CDS             complement(1700..>2170)
                     /locus_tag="VLR27_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP03625.1"
                     /translation="AVDDLDLTVKHIRSVIFGLDASRRGESGMRDRILALTQEAAGPL
                     GFGPRVAFDGPVDTLVPEHAESDLLATLREALANAARHAQARTVDVRVLATTEQVELT
                     VRDDGIGGAATRSGGRGLRNMHTRAERLGGTCSIEDGPEGGTLLTWCVPVEPPA"
BASE COUNT          266 a          804 c          787 g          313 t
ORIGIN      
        1 accacggtgg ccgacggcct ccggcgcgat ggccacgaga tcgtggacct gtgcctgccc
       61 gatcccgcct ccccgctcac gtgccggggc ctggtcgagc accgctgccc gctcgacgaa
      121 ggggcggatc tggccatcac cgcgctgcag cacccccagg acgccccgtc ggccggcacc
      181 gtgtgcgccc gccgggacgg catccccgtc gtggccctcg gccgccacga cgtcagccac
      241 cccgatctcg gttcggccct cgcccgggtc gccgagctcg gcgacgacca cgtcaccgcc
      301 accgtccagg tcgcgctgca gcaggtgctc accgagatgg gcatcgaagg cggcgacgtc
      361 gtcctgcacc gcgacgcggt gtgcgagcag atcgtggcga cggtgccggg tccgctcgac
      421 gacccgaccc gctcccgcat cgccgtccgg ctcctcgacg ccgccacggc ctcgggtcgc
      481 cacgggcgcc ggcgcgacgt cgccgtgcgc tgcgcctagg agggcttcag tcccggtgga
      541 gctcgcctcg ctcgtcgagg cgggccgccc acacggcagc ctccgtgcgc tggctcatgc
      601 cgagcttggc cagcacgttg gagacgtagt tcttcaccgt cttctcggcc aggaacatgt
      661 cggcaccgat ctcgcggttc gtgcgcccct cggcgagcag ctccacgatg cggtgctcct
      721 ggggggtgag ccgctcgagg cgctcgtcgc gctgctcccg gttgcgcacg gcgcgcagca
      781 cccgttcggt gacggccgga tcgagcagcg actgccccgc cgcgacacgc cgcacggcgt
      841 cgatgaggtc gaggcccttc acctgcttga gcaggtaccc ggaggcaccg gcgaggatgg
      901 cgttgagcag cgcgtcctcg tcgtcgaagg acgtgagcat caggcactgg atcgtgctgt
      961 cccgctcgcg caccgcccgg gcgatgtcga tgccggtgcc gtcgggcagg cgcacgtcga
     1021 ggacggcgac gtcgacgttg ctgcccggga tgcggccgat ggcgtcctcg acggtcccgg
     1081 cctcgccggc gatctcgatg tcgtcgaccg cgtcgagcag gtccatcaac ccgcgccgca
     1141 cgacctcgtg gtcgtcgacg acgaagaccc ggatcgggat ggtgctcatg gcgaccatcc
     1201 tcgcacttct agccggttcg ctggtgaccg aggggcgcag ggcgccttcg ggacctatga
     1261 ccctgggcag ggagcgcggc gccagccacc atcgtcgccg tgtcggaggt gtggaccggg
     1321 ttccagacga tgacggcgga cgagtgccgt cgcctgctcg ccacggtcga catcggccgg
     1381 gtcgcgatca gcgcgggcgc gctgccgttc gtgctgcccg tgcagtacgc gctgcaggac
     1441 gaccacctcc tgatgcggac gcccggccac cacgaggtgg gcgacggcat cgacgggcag
     1501 gtggtcggct tcgaagccga cacgatcgac ctcgaccacg gcgtgggctg gtgcgtgtcg
     1561 gtgaccggca ccgtccgcgt cgtgcccgac gcgccgatgg cggaccccgt gcatcgctgg
     1621 ttctccgacg gcgtggtgct cgccctgtcc accgacgtca tcgtcggcca ccgcgtcgcg
     1681 gtctgagtgg ggcctccggc taggccggcg gttcgacggg cacgcaccag gtgagcaggg
     1741 tgccgccctc gggcccgtcc tcgatgctgc acgtgccgcc caggcgctcg gcccgggtgt
     1801 gcatgttgcg cagcccccgc ccgccggatc gggtcgcagc gccgccgatc ccgtcgtcgc
     1861 gcaccgtgag ctcgacctgc tcggtcgtgg ccagcacccg cacgtcgacg gtgcgggcct
     1921 gcgcgtggcg cgccgcgttg gccagggcct cgcgcagggt ggcgagcagg tcggactcgg
     1981 cgtgctcggg caccagggtg tcgaccgggc cgtcgaaggc cacccggggc ccgaagccga
     2041 gcggcccggc ggcctcctgg gtgagggcga ggatccggtc gcgcatgccc gactcgcctc
     2101 gccgggacgc gtcgaggccg aagatgaccg accggatgtg cttcaccgtg aggtcgaggt
     2161 cgtcgaccgc
//
LOCUS       DATISU010000218         8884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_383683, whole genome shotgun sequence.
ACCESSION   DATISU010000218 DATISU010000000
VERSION     DATISU010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8884
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383683"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..291)
                     /locus_tag="VLR27_09010"
     CDS             complement(4..291)
                     /locus_tag="VLR27_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03626.1"
                     /translation="MADDGTEHVDLTASTRSPALPVLDDPRVRTATKALAAVVAFYFV
                     QQRLWPAPAGVLLQGLVLGGLTALISFGIAMIYRSNRIINFSAVELGAVPA"
     gene            448..1326
                     /locus_tag="VLR27_09015"
     CDS             448..1326
                     /locus_tag="VLR27_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03627.1"
                     /translation="MGAIADKRLGDFLPTIDAYRPVAIAVAAILGLVVVVPGAVRDSG
                     GPIADFSSTPAADGEDLAAPATDGGDGTPDRAPSSSSAAPAPTPTARPAVGAQPVRAT
                     SPAPRADAASPSGGGARAGSPPAAIVPDADGGPAGVGSESLRIAAAGWASSTGGTPVG
                     ATVESIPEGALPVGTRVGQTDKVAFVRLAGDATALVLAEDAAGRRGSAFDASPVQLCQ
                     VTAAGWEAGEDQAMSDAPPHDPDLCAPGQQGADGTWSFTLTLFEDPTDDRGLALVPTA
                     DAPLDFQITFAGQALG"
     gene            1390..1968
                     /locus_tag="VLR27_09020"
     CDS             1390..1968
                     /locus_tag="VLR27_09020"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HSP03628.1"
                     /translation="MTTIPADRPRASVFIATSVDGYIARPDDGLDWLTGQPDDELTGE
                     HDPGQDFGYAEFFASVDALLMGRGTFDVVQGFAGWFYGDKPVYVWTSHPDDITPKDGA
                     VVRPVAGSLDDVVARLVSDGIGHVYVDGGKVIRQFLAAGLIDRMTISTPPVLIGEGIP
                     LFGGTGEDIRLELVSCETFDGGMVQRVYDVVR"
     gene            2010..2780
                     /locus_tag="VLR27_09025"
     CDS             2010..2780
                     /locus_tag="VLR27_09025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP03629.1"
                     /translation="MDRPQRLVFGSVAEAYDAHRPGYPDELLDDLVAMVGSDARALDA
                     GAGTGKVAAALAARGLTGTALEPDPDMAAVARRHVEASGWAVLVAELEAADLAAGAHD
                     LFTCAQAWHWIDQEQGLAQVRHALRPGGVAAIFWNRPGFPDAELRVRLDEVYARLAPE
                     MESSLASKASGHKGVPPAVDPAPEGFTAAEARTYHHAIRYTTARWVGYLGTHSNHVLL
                     GDEQRTALHRAVAEVIDDHGGELEVAYRCEAWIARRLG"
     gene            complement(2803..3693)
                     /locus_tag="VLR27_09030"
     CDS             complement(2803..3693)
                     /locus_tag="VLR27_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSP03630.1"
                     /translation="MTLSFDGKVAIITGAGGGLGRSHALELARRGAHVVVNDLGGSVD
                     GVGGDDSAAQKVVDEIKAAGGEAVANHDSVATPEGGKAIVQTALDTWDKVDIVINNAG
                     ILRDTSFAKMTPEQLNPVLDVHLKGAFYVTQPAFVQMKEQGFGRIVNTASGAGIFGNF
                     GQTNYGAAKMGLVGFTNVLAQEGAKYNIKANAIAPVAATRMTEGLLGPLADKLGPEYI
                     TPVAVYLAHESCEVTGEIYSCGGGRVARIFVGVTPGIVDDELSVEMMGERLAEIRAED
                     GYEIPTSMNEEMGIAIKAMS"
     gene            complement(3711..5345)
                     /locus_tag="VLR27_09035"
     CDS             complement(3711..5345)
                     /locus_tag="VLR27_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP03631.1"
                     /translation="MPGWNFADAWETVAETLPDAPALLHGDLVRSWSDFDARADGVAA
                     ALLEAGVTEGTSVAQYLTNRPEYLEAVFASFKLGLAPVNTNYRYVEDELLYLWDNADS
                     SAVVFEGQFTERIEHIRDRVPDVRVWLWVDDGHGECPDWATSFEAAATSGAGRTSAPW
                     GRSGDSLYLLYTGGTTGMPKGVMWRNDDLLLVLNDGAPRARKLPEQVDLDHLRQVVSS
                     PGIRTLPACPLMHGTGAFNAFTALTVGGSVVTLTKSSFDAVELLDAVQDKQVNNTAIV
                     GDAFARPILAALDAEPDRWDISSLKVVVSSGVMWSQASKDGLLRHNPRMLLVDTLGSS
                     EAIGMASSVSSGSGGGASSARTATFTLGPSTKVIADDGTEVRPGDDAIGKIAIKGRTP
                     VGYYKDPTKSAETFVRIGDATYSMPGDYARVEADGTLVLLGRGSVCINTGGEKVFPEE
                     VEEALKEHADVRDAVVVGLPDERFGQAITAVVEAVPGTDPSPDELIATVKAHHAAYKA
                     PKRIVFVDSLSRAANGKVDYKRWTRHAAGVLGVDEG"
     gene            5415..6668
                     /locus_tag="VLR27_09040"
     CDS             5415..6668
                     /locus_tag="VLR27_09040"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP03632.1"
                     /translation="MRQLLAGVAVDITPLRRSRDFRYLYAGTAVGQIGSAFTTVAVPF
                     QIYDLTGSTLAIGLFGGITLIPLLTMNILGGAIADKVERRRWIVGCQLLGLVGTAGLA
                     LNATLDSPNLPAIYALGALDICAFALGAPAERASVIRVVGREHVPSAMALKAVSSSVA
                     RVAGPALAGVLLATAGTTWTYLVDASTFVIAAVVISRMRPLQPQIVGGHPTLGLILDG
                     FRHLRRQPVLLGSFISDLNAMVLGAPVALFPAVAATRFADDPQVLGLLYAAPFAGSLV
                     ASLFSGWARSITRHGRVVTVCIIVWGLAMAAFGLVDGVLLSLGALAIGGAADMISGIS
                     RQAILQLASPPDLVGRMEGVGMAVWSGGPRLGELESGVVASLTSVQFSIVSGGLGCVA
                     VMVLLARWLPGFANYEVPREPAPAA"
     gene            complement(6670..8235)
                     /locus_tag="VLR27_09045"
     CDS             complement(6670..8235)
                     /locus_tag="VLR27_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03633.1"
                     /translation="MRGRGRLVEHVVRGAVVALALSSLPMLSTTPVGAQSAADALEAC
                     DPLTAVPELGPRACGGLERATWLAAQHCRRVPPAQEPVCPSIDGRPVHEPAMAAFEEG
                     WVARALDLQRELDLDVPLTEALILHTHNSGNSAAYDPSVTINDANQVVSVTDQLRLGI
                     RGIELDLHWTPHPTGDPGEGFRAVVQCHGQSVDTPAGSVHPGCSVDRTFDAHLAELAA
                     WLDRPEAAGELVVLYLENQLDGADAAHASAVASLERHLGDVLLRPDAGAGCQELPVTR
                     TKREILATGARVLLTGNCGPAGWTDLVFQRGPAWDEGGAVTDYPTGTVCEAEREAHDY
                     DRTFVRRFEDSTFLSLTVNGGSHITPEVAAAMARCGVNFPGLDQVHPGDERLPALVWS
                     WRADADPDAPDAACAAQGDDGRFGPLPCDTVLPVACRTDDGGWAPSSRSVRWAAGDRA
                     CRADGHVGAGVPANGWENELLRRSSDGLGDVWLAYARQPRPDGSERWTTGMPEREPPT
                     GRGQRGGRPLRAF"
     gene            complement(8474..>8884)
                     /locus_tag="VLR27_09050"
     CDS             complement(8474..>8884)
                     /locus_tag="VLR27_09050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP03634.1"
                     /translation="RPPTLDELSVEVGHPPAKIEEILASARIAKSLDDGLSDDGDMTL
                     GDLAAVAEDDPEGDAVTEATRGDVLAGLKGLDEREQRILILRYGLDGHEPRTLDEVGR
                     HFDLTRERIRQIEARAMSKLRHPANAGILADLAS"
BASE COUNT         1121 a         3146 c         3334 g         1283 t
ORIGIN      
        1 cagctacgcc gggacggcgc cgagctccac ggcggagaag ttgattatcc ggttcgagcg
       61 gtagatcatg gcgatgccga aggagatcag cgccgtgagc ccaccgagca cgagcccctg
      121 cagcagcacg ccggccggtg ccggccacag tcgctgctgg acgaagtaga aggcgacgac
      181 cgcggcgagc gccttggtcg ccgtccgcac tcgtggatcg tcgagcacgg ggagcgccgg
      241 cgagcgcgtg ctcgccgtca ggtccacgtg ctcggtgccg tcgtcggcca tcgaaccccc
      301 tgggcggcca cgcccgaccc ccggacgtgg ctcccgtcgg ctgattcgct gtcgccggcg
      361 ggcgttcctc cccggcggca gaagttcttg acccgagggt caggacggag caggaaccgg
      421 gggccccggc agcgaagcag ggcgcgcatg ggggcaatcg cggacaagcg gctgggggat
      481 ttcctgccga cgatcgacgc gtaccggccg gtggcgatcg cggtcgcggc catcctcggg
      541 ctggtcgtgg tggttcccgg tgcggtccgg gactccggcg gaccgatcgc ggacttctcg
      601 tcgacaccgg ccgccgacgg agaggacctg gccgctccgg cgaccgacgg tggggacggg
      661 acgccggatc gggcgccgtc gtcatcgtcg gcggcaccgg caccgacgcc gaccgcacgc
      721 ccggcggtgg gtgcccaacc ggtacgcgcc acctcgcctg cgcctcgcgc cgacgcagcc
      781 tccccgtcgg ggggtggggc ccgggcaggc agtccgccgg cggccatcgt ccccgacgcc
      841 gacgggggcc ccgccggggt cggttcggag tccctgcgca tcgcggcagc cgggtgggcg
      901 tcctcgacgg gtggcacccc cgtcggcgcc acggtggaga gcatccccga aggagcgctg
      961 ccggtcggca cacgggtggg gcagaccgac aaggtggcct tcgtccgcct cgccggcgat
     1021 gccacggccc tggtcctggc cgaggacgcc gccggccgcc ggggcagcgc gttcgacgca
     1081 tcgccggtcc agctgtgcca ggtcaccgca gccgggtggg aagccggtga ggaccaggcg
     1141 atgtccgacg ccccgccgca cgacccggac ctctgcgctc ccggccagca gggggccgac
     1201 ggcacgtgga gcttcaccct cacgctgttc gaggacccga ccgacgaccg cgggctcgcg
     1261 ctcgttccca cggcggacgc gccgctcgac ttccagatca ccttcgccgg gcaggcgctg
     1321 ggctgacgtt ccgctgccgc tgccagaaca cccggggggc gggtcacggc ggggtcgtac
     1381 cgttggttcg tgaccaccat ccccgctgac cggccgcgcg cgtcggtctt catcgccacc
     1441 agcgtcgacg gctacatcgc ccgacccgac gacggcctcg actggctcac cggccagccc
     1501 gacgacgagc tcacgggcga gcacgacccc ggtcaggact tcggctacgc cgagttcttc
     1561 gccagcgtgg acgcgctcct catggggcgc ggcacgttcg acgtcgtcca gggcttcgcc
     1621 ggctggttct acggcgacaa gcccgtctac gtctggacga gccaccccga cgacatcaca
     1681 ccgaaggacg gggcggtggt gcggcccgtc gccggttcgc tcgacgacgt cgtcgctcgc
     1741 ctggtgtccg acggcatcgg ccacgtctac gtcgacggcg gcaaggtgat ccgccagttc
     1801 ctggccgccg ggctcatcga ccggatgacc atctcgaccc caccggtgct catcggcgag
     1861 ggcatcccgc tcttcggcgg caccggcgag gacatccgcc tcgagctggt gtcctgcgag
     1921 accttcgacg gcggcatggt gcagcgggtc tacgacgtgg tgcgctgatc ccgggagcag
     1981 ccgccgcccg accgtgcgac gatggcgtcg tggaccgacc ccagcgactc gtgttcggct
     2041 ccgtcgccga ggcctacgac gcccaccgtc ccggctaccc cgacgagctc ctcgacgacc
     2101 tggtggcgat ggtcggctcc gatgcccgtg cgctcgacgc cggcgccggc accgggaagg
     2161 tcgccgccgc gctggcggcg cggggcctca ccggcacggc gctcgagccc gatccggaca
     2221 tggccgccgt ggcccgccgc cacgtggagg cgtccggctg ggcggtcctg gtcgccgagc
     2281 tcgaggcggc ggacctggcg gccggagcgc acgacctgtt cacctgcgca caggcctggc
     2341 actggatcga ccaggagcag gggctcgccc aggtccgcca cgccctccgc cccggtggcg
     2401 tggccgccat cttctggaac cggccggggt tccccgacgc ggagctccgc gtccggctcg
     2461 acgaggtcta cgcccgcctg gcgccagaga tggagtcgtc cttggcctcc aaggccagcg
     2521 ggcacaaggg ggtgcccccg gccgtggatc cggcgccgga gggattcacc gcggccgagg
     2581 cgcgcaccta ccaccacgcc atccgctaca cgaccgctcg ctgggtcggg tacctcggca
     2641 cccactcgaa ccacgtgctg ctcggtgacg agcaacggac cgccctgcac cgggccgtgg
     2701 ccgaggtgat cgacgaccac ggcggcgagc tcgaggtcgc ctaccggtgc gaggcatgga
     2761 tcgcccggcg cctcgggtga ggcgaggcgc cgggcgaccc gatcagctca tcgccttgat
     2821 ggcgatgccc atctcctcgt tcatcgacgt ggggatctcg tagccgtcct cggcccggat
     2881 ctcggcgagg cgctcgccca tcatctccac cgacagctcg tcgtcgacga tgcccggggt
     2941 gacgcccacg aagatgcggg cgacccggcc gccaccgcag ctgtagatct cgccggtgac
     3001 ctcgcaggac tcgtgcgcca ggtacacggc cacgggggtg atgtactcgg ggccgagctt
     3061 gtcggcgagc gggccgagca ggccctcggt catgcgggtg gcggcgacgg gcgcgatggc
     3121 gttggccttg atgttgtact tcgcgccctc ctgggcgagg acgttggtga agccgaccag
     3181 gcccatcttc gccgcgccgt agttggtctg gccgaagttg ccgaagatgc cggcgccgga
     3241 ggcggtgttg acgatgcggc cgaagccctg ctccttcatc tgcacgaagg cgggctgggt
     3301 cacgtagaac gcgcccttga ggtgcacgtc gagcaccggg ttgagctgct cgggtgtcat
     3361 cttggcgaag ctggtgtcgc gcaggatgcc ggcgttgttg atgacgatgt cgaccttgtc
     3421 ccaggtgtcg agcgccgtct ggacgatggc cttgccgccc tcgggggtgg caaccgagtc
     3481 gtggttggcg acggcctcgc caccggcggc cttgatctcg tcgaccacct tctgggcggc
     3541 ggagtcgtcg ccgccgacgc cgtcgacgga gccgccgagg tcgttgacga ccacgtgggc
     3601 gccgcgccgg gccagctcga gtgcgtggga gcggccgagg ccaccgcctg cgccggtgat
     3661 gatcgcgacc ttgccgtcga aggagagagt catgccggcg aggctacgac tcacccttcg
     3721 tccactccca aaaccccggc ggcgtggcgg gtccagcgct tgtagtcgac cttgccgttg
     3781 gcggcccggg acagcgaatc gacgaagacg atgcgcttgg gggccttgta ggcggcgtgg
     3841 tgggccttca ccgtggcgat gagctcgtcg gggctggggt cggtgccggg gacggcctcg
     3901 accacggcgg tgatggcctg gccgaagcgc tcgtcgggga gcccgacgac cacagcgtcg
     3961 cggacatccg cgtgctcctt gagggcctcc tcgacctcct cggggaagac cttctcgccg
     4021 ccggtgttga tgcacaccga cccgcggccg aggagcacga gggtgccgtc ggcctcgacc
     4081 cgggcgtagt cgccgggcat ggagtaggtg gcgtcgccga tgcggacgaa ggtctcggcc
     4141 gacttggtcg ggtccttgta gtagccgacc ggcgtgcgcc ccttgatggc gatcttgccg
     4201 atggcgtcgt cgccggggcg gacctcggtg ccgtcgtcgg cgatgacctt ggtggatgga
     4261 cccagggtga acgtggccgt gcgagctgag ctggcgccgc ccccggaccc gctggacacc
     4321 gagctggcca tgccgatggc ctccgacgag ccgagcgtgt cgacgagcag catccgcggg
     4381 ttgtggcgga gcaggccgtc cttgctggcc tggctccaca tgacacccga ggacacgacg
     4441 accttgagcg aggagatgtc ccagcggtcg ggctcggcgt cgagggcggc gaggatcggg
     4501 cgggcgaagg cgtcgccgac gatggcggtg ttgttcacct gcttgtcctg gaccgcgtcg
     4561 agcagctcga cggcgtcgaa gctggacttc gtcagggtca cgaccgatcc gccgacggtc
     4621 agggcggtga aggcgttgaa cgcgccggtg ccgtgcatga gcgggcaggc cgggagcgtg
     4681 cggatgccgg gggaggagac gacctggcgg aggtggtcga ggtcgacctg ctcgggcagc
     4741 ttgcgggccc gcggtgcgcc gtcgttgagc acgaggagca ggtcgtcgtt gcgccacatc
     4801 acgcccttgg gcatgccggt ggtgccgccg gtgtagagca ggtagagcga gtcgcccgag
     4861 cggccccacg gcgcgctggt ccggccggcg ccgctggtgg cggcggcctc gaaggacgtg
     4921 gcccagtccg ggcactcgcc gtggccgtcg tcgacccaca gccagacccg cacgtccggc
     4981 acccggtcgc ggatgtgctc gatgcgctcg gtgaactggc cctcgaagac caccgccgag
     5041 gagtcggcgt tgtcccagag gtagagcagc tcgtcctcga cgtagcggta gttcgtgttc
     5101 accggcgcca gcccgagctt gaacgaggcg aacacggcct cgaggtactc gggccggttg
     5161 gtgaggtact gcgccacgct cgtgccctcg gtgacgccgg cctcgaggag ggcggcggcc
     5221 accccgtcgg ccctggcgtc gaagtcgctc caggaccgca ccaggtcgcc gtgcagcagg
     5281 gcgggggcgt cggggagggt ctcggcgacc gtctcccagg catcggcgaa gttccatccg
     5341 ggcatggggc gatcgtacgg acgaagcctt ccggattcct cactaccgac cggtagcgtt
     5401 ccccggtgcg cggcatgagg cagctgctgg cgggcgtggc cgtcgacatc acccccctcc
     5461 ggcgatcccg agacttccgg tacctgtacg ccggcaccgc cgtgggtcag atcggctcgg
     5521 ccttcacgac cgtcgccgtg ccgttccaga tctacgacct gaccggttcg accctggcca
     5581 tcgggctctt cggcggcatc acgctcatcc cgctgctgac catgaacatc ctcggcggcg
     5641 ccatcgccga caaggtcgag cgacgtcgct ggatcgtcgg gtgccagctg ctcggcctgg
     5701 tcggcacggc cggtctcgcc ctgaacgcca ccttggactc gccgaacctg ccggccatct
     5761 acgcgctcgg tgccctcgac atctgcgcgt tcgcgctcgg cgcgccggcc gagcgggcgt
     5821 cggtgatccg ggtggtgggc cgggagcacg tgccctcggc catggcgctc aaggcggtgt
     5881 cgagcagcgt ggcccgcgtg gccggtccgg cgctcgccgg cgtgctgctg gcgacggccg
     5941 gcacgacctg gacctacctg gtcgacgcgt ccaccttcgt gatcgccgcc gtggtgatct
     6001 cgcggatgcg ccccctgcag ccccagatcg tcggcggtca cccgaccctc gggctcatcc
     6061 tcgacggctt ccggcacctg cgccgccagc ccgtcctgct cgggtccttc atctccgacc
     6121 tcaacgccat ggtcctcggg gcgccggtgg cgctcttccc ggccgtggcc gccacccgct
     6181 tcgccgacga cccccaggtg ctcggcctgc tctacgccgc ccccttcgcc ggttcgctgg
     6241 tcgccagcct gttcagcggt tgggctcgga gcatcacccg gcacggccgg gtcgtgaccg
     6301 tctgcatcat cgtgtggggc ctggccatgg ccgcgttcgg cctggtcgac ggggtcctgc
     6361 tgtcgctcgg ggccctggcc atcggcggcg cggccgacat gatctcgggc atctcccggc
     6421 aggcgatcct gcagctcgcc agcccgccgg acctggtcgg gcgcatggag ggcgtgggga
     6481 tggcggtctg gtcgggcggc ccgcgcctgg gcgagctcga gtccggggtg gtcgcgtcgc
     6541 tgacgtccgt gcagttctcg atcgtgtccg gcggtctcgg ctgcgtcgcc gtcatggtgc
     6601 tgctggcccg gtggttgccg gggttcgcca actacgaggt gccccgggag ccggccccgg
     6661 cggcgtgagt cagaacgccc gcagcggccg cccgccccgc tgcccccggc ccgtcggagg
     6721 ctcccgctcc ggcatgcccg tggtccatcg ctcgctcccg tcgggacggg gctggcgggc
     6781 gtaggccaac cagacgtcgc ccaggccgtc ggagctccgg cgcagcagct cgttctccca
     6841 gccgttggcc ggcacgccgg cgccgacgtg gccatcggcg cggcacgccc ggtcgcccgc
     6901 cgcccagcgg acggaccggc tcgacggtgc ccatccgccg tcgtccgtgc ggcaggccac
     6961 gggcagcacg gtgtcgcagg ggagcggtcc gaagcggccg tcgtctccct gcgcggcaca
     7021 cgccgcgtcg ggggcatccg ggtcggcgtc ggcccgccag ctccacacga gggcgggcag
     7081 gcgctcgtcg ccggggtgca cctggtcgag gccggggaag ttgacgccgc agcgcgccat
     7141 ggcggccgcg acctcggggg tgatgtggga gccgccgttc accgtcaggg acaggaacgt
     7201 ggagtcctcg aaccggcgga cgaaggtgcg gtcgtagtcg tgggcctcgc gctcggcctc
     7261 gcagaccgtg ccggtggggt agtcggtgac ggcgccgccc tcgtcccagg ccggcccccg
     7321 ctggaacacc aggtcggtcc acccggccgg gccgcagttg ccggtgagca ggacccgggc
     7381 gcccgtggcg aggatctccc gcttggtccg ggtcaccggc agctcctggc agccggcgcc
     7441 ggcgtccggt cggagcagca cgtcgccgag gtggcgctcg agggaggcca ccgcggaggc
     7501 gtgggcggcg tcggccccgt cgagctggtt ctccaggtag agcacgacca gctcgccagc
     7561 cgcctcgggg cgatcgagcc aggcagccag ctcggcgagg tgggcgtcga aggtgcgatc
     7621 gaccgagcag ccggggtgca ccgagccggc gggggtgtcg acggactgcc cgtggcactg
     7681 cacgacggcg cggaacccct ccccggggtc gccggtcgga tgcggggtcc agtgcaggtc
     7741 gagctcgatg ccgcggatgc cgagccggag ctggtcggtg accgacacga cctggttggc
     7801 gtcgttgatc gtgaccgacg ggtcgtaggc cgccgagttg cccgagttgt gcgtgtgcag
     7861 gatcagggcc tcggtgagcg gcacgtcgag gtccagctcg cgctgcaggt cgagtgcccg
     7921 ggcaacccag ccctcctcga aggcggccat ggcgggttcg tggaccggcc ggccgtcgat
     7981 cgaggggcag acgggctcct gggccggggg gacgcggcgg cagtgctggg cggcgagcca
     8041 cgtggcccgc tcgagcccgc cgcatgcccg cgggccgagc tcgggcacgg cggtcagcgg
     8101 gtcgcaggcc tcgagcgcgt cggcggcgga ctgggcgccg accggcgtgg tggacagcat
     8161 cggcagcgac gagagggcaa gggccaccac ggcgccccgc accacgtgct cgaccaaccg
     8221 acctcgaccc cgcatcacgc ctcccgtccg gacctcacgg ggtggttcgc caccggcggg
     8281 gtcgacccct gcccggacgc gacgacgccc cggccgcagc cggggcgtcg atcaggtcgt
     8341 ggtagctggc gctacaccag cactgccagt tcttcagagg cgagccgcca ggcgagccga
     8401 ccaatgtcac ggttcgttgc ggcggcggag ccgccaacat tcaccgcgct ggggtgcaac
     8461 ccactcgcca gcgtcaggag gcgaggtcgg cgaggatgcc ggcgttggcc gggtggcgca
     8521 gcttggacat ggcccgggcc tcgatctggc ggatgcgctc ccgggtgagg tcgaagtggc
     8581 gaccgacctc gtcgagggtg cggggctcgt ggccgtcgag gccgtagcgc aggatcagga
     8641 tgcgctgctc gcgctcgtcg aggcccttga gcccggcgag cacgtcgccg cgggtggcct
     8701 cggtgacggc gtcgccctcg gggtcgtcct cggccacggc ggcgaggtcg ccgagggtca
     8761 tgtcgccgtc gtcggagagg ccgtcgtcga gggacttggc gatgcgggcc gaggcgagga
     8821 tctcctcgat cttggccggc gggtggccga cctcgacgga gagctcgtcc agtgtgggcg
     8881 ggcg
//
LOCUS       DATISU010000219         1644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_849737, whole genome shotgun sequence.
ACCESSION   DATISU010000219 DATISU010000000
VERSION     DATISU010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849737"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VLR27_09055"
     CDS             complement(<1..309)
                     /locus_tag="VLR27_09055"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSP03635.1"
                     /translation="MAFLHDLPEKLPERMARHVEADEAGGIAWLASRHGKVVAGSTGV
                     LTRGEPAPVERDSLFRIASVTKPIVAVAALLLVEECRLRLEDPVDDLLPELADRRVLV
                     D"
     gene            complement(352..>1644)
                     /locus_tag="VLR27_09060"
     CDS             complement(352..>1644)
                     /locus_tag="VLR27_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSP03636.1"
                     /translation="ERAIFNAMIDRRPAIIAKCTSVRDVQDALALARDKDMPVAVRAG
                     GHSVAGFCLNDGGVVIDVRRMNAVDVDPERRTARVGAGATWGEFDRATQEHGLATTGG
                     RVSTTGVAGLTLGGGSGWLERRHGLACDNLVSVDLVTADGRTVTASEDEHPELFWALH
                     GGGGNFGIATSFEFALHPVGPEVLAGLLVWPGDAGYEVSRSYRDMAFDAADELGSALV
                     FLTGPPEEFIPEHLQGQTLAAVALLWAGDVAEGEDAIADLRALRPEVDLVGPMPYADF
                     QCMIDDPPGLGNYWSADYHDTFPDDALDIFVKYGFERPSPASQQLLVPWGGAIARVPD
                     DASPMTHRDVRWISHPFAMWELAEDATANIDWAQSFRRDVSSHTNGGVYLNFIGHEGE
                     ARIKAAFGEVKYERLQQVKAEWDPDNVFQGNQNIRPAV"
BASE COUNT          229 a          550 c          616 g          249 t
ORIGIN      
        1 gtcgacgagg acccgtcggt cggcgagttc gggcaggagg tcgtcgacgg ggtcctcgag
       61 gcggaggcgg cactcctcga ccagcagcag cgcagcgacg gcgacgatcg gcttcgtcac
      121 cgaggcgatg cggaacagcg agtcccgctc gaccggtgcc ggctcgcctc gcgtgagcac
      181 accggtcgat ccggcgacga ccttcccgtg gcgtgacgcg agccaggcga tgccgcccgc
      241 ctcgtcggcc tcgacgtggc gggccatccg ctcgggcagc ttctcgggga ggtcgtgaag
      301 gaaggccatg tccttccgac gttgccgtgc cggtcagatc atcgggatcg ctcagaccgc
      361 ggggcggatg ttctggttgc cctggaagac gttgtccggg tcccactcgg ccttgacctg
      421 ttgcaggcgc tcgtacttga cctccccgaa cgccgccttg atgcgcgctt cgccctcgtg
      481 gccgatgaag ttgaggtaga cgccaccgtt cgtgtgcgag gagacgtcgc ggcggaagct
      541 ctgggcccag tcgatgttgg cggtggcgtc ctcggccagc tcccacatgg cgaaggggtg
      601 gctgatccag cggacgtcgc ggtgggtcat cgggctggcg tcgtcgggga cgcgggcgat
      661 ggctccaccc cacgggacga ggagctgctg gctggccggg gacggccgct cgaagccgta
      721 cttcacgaag atgtcgagcg catcgtcggg gaacgtgtcg tggtagtcgg cgctccagta
      781 gttcccgagc ccgggcggat cgtcgatcat gcactggaag tcggcgtagg gcatggggcc
      841 gacgaggtcg acctccggcc ggagggcgcg gaggtcggcg atcgcgtcct cgccttcggc
      901 gacgtcgccg gcccacagga gggcgactgc ggcgagggtc tggccctgga ggtgctccgg
      961 gatgaactcc tcgggcgggc cggtgaggaa caccagcgct gatccgagct cgtcggcggc
     1021 gtcgaaggcc atgtcgcgat aggagcggct cacctcgtag ccggcgtctc ccggccacac
     1081 gagcagcccg gcgagcacct ccggtcccac cgggtggagc gcgaactcga acgacgtggc
     1141 gatgccgaag ttgcccccgc cgccgtggag cgcccagaac agctccgggt gctcgtcctc
     1201 gctggcggtc acggtgcgac cgtcggcggt gaccaggtcg accgagacca ggttgtcgca
     1261 ggcgaggccg tgacggcgct cgagccaccc ggaaccgccg ccgagggtga gtccggccac
     1321 tccggtggtc gacacgcgac cgccggtggt ggcgaggccg tgctcctgcg tggcccggtc
     1381 gaactcgccc cacgtcgcac cggccccgac gcgagcggtc ctccgctccg gatccacatc
     1441 gacggcgttc atgcggcgga cgtcgatcac gacgccgccg tcgttgaggc agaagccggc
     1501 gacggagtgt ccgccggccc gcacggcgac cggcatgtcc ttgtcccggg cgagcgcgag
     1561 cgcgtcctgg acgtcgcgca cggatgtgca cttggcgatg atcgccggtc gccggtcgat
     1621 catcgcgttg aagatggcgc gctc
//
LOCUS       DATISU010000220         1768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_616642, whole genome shotgun sequence.
ACCESSION   DATISU010000220 DATISU010000000
VERSION     DATISU010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616642"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1236
                     /gene="dxr"
                     /locus_tag="VLR27_09065"
     CDS             67..1236
                     /gene="dxr"
                     /locus_tag="VLR27_09065"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HSP03637.1"
                     /translation="MTTISIAGSTGSIGTQTLEVVAAEPHRFEVIALGAGSSVEAVIE
                     QANAVRPQVVAIADASRAAEVEAGVPAGTEVVAGPDALAAISGVADVCVNGVVGFAGL
                     PVTLGALQAGKRLALANKESLIAAGPVVREARRTPGADLVPVDSEHCAVHMCLRGSDG
                     GRRVHEIVLTASGGPFRGRTRADLAEVTVDDALAHPTWSMGPKITVDSSTLMNKGLEV
                     IEAHELFAEDFGVGFDDIDVVVHPQSIVHSMVTYSDGATIAQLSMPDMRLPIGYALAH
                     PDRLATPFGPIDWGSLARLDFEAPDREAFPCLDLAYRAGRAGGLAPATLNAANEVAVE
                     AFLAGRIRWIDIPDVLSAVLDRHNGGTASTLDDVLGADADARRLATRLVEERFTA"
     gene            1233..>1768
                     /locus_tag="VLR27_09070"
     CDS             1233..>1768
                     /locus_tag="VLR27_09070"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSP03638.1"
                     /translation="MTSLQHPPSQPAQPPTPPPPPGRNPDEERGTSPVRLAILAGLVA
                     WLGIAGGWAPLVVVFGVVVMIFLHELGHYLAAKWGGMKVTEFFIGFGPRIWSFQRGET
                     EYGLKVIPAGAYVKVIGMNNLDEVDPADEGKTYRQASYPRRMAVAVAGSTMHFLQAIL
                     LLFVLYSFVGVPGGSLTE"
BASE COUNT          233 a          699 c          572 g          264 t
ORIGIN      
        1 tcgtgctgcc ggccgtgtac tacgtggcca aggtgtccga cttcttcctg tgatctgatc
       61 gcacccgtga ccaccatctc gatcgccggc tccaccgggt cgatcggaac ccagaccctc
      121 gaggtcgtcg cggccgagcc ccaccgcttc gaggtcatcg ccctcggcgc cggctcgtcg
      181 gtcgaggccg tcatcgagca ggccaacgcc gtgcgccccc aggtcgtggc catcgccgac
      241 gcctcgcgcg ccgccgaggt cgaggccggg gtgccggcgg gcaccgaggt cgtggccggc
      301 cccgacgccc tcgccgccat cagcggcgtc gccgacgtgt gcgtgaacgg cgtggtcggg
      361 ttcgccggcc tgcccgtcac cctcggtgcg ctccaggccg gcaagcgcct cgcgctggcc
      421 aacaaggagt ccctcatcgc cgccgggccc gtcgtgcgcg aggcccgccg caccccgggt
      481 gccgacctcg tgccggtgga ctccgagcac tgcgcggtgc acatgtgcct gcggggcagc
      541 gacgggggac gccgggtcca cgagatcgtg ctcaccgcgt ccggcgggcc attccgcggg
      601 cgcacccggg ccgatctggc cgaggtcacc gtcgacgacg ccctcgccca ccccacctgg
      661 agcatggggc ccaagatcac cgtcgattcg tccaccctca tgaacaaggg gctggaggtc
      721 atcgaggccc acgagctgtt cgccgaggac ttcggggtcg gcttcgacga catcgacgtc
      781 gtcgtgcacc cccagtccat cgtccactcg atggtcacct acagcgacgg ggccaccatc
      841 gcccagctgt cgatgcccga catgcgcctg cccatcgggt acgccctggc ccaccccgac
      901 cgcctcgcca cgcccttcgg gcccatcgac tggggctccc tcgcccgcct cgacttcgag
      961 gcgcccgacc gggaggcgtt cccgtgcctc gacctcgcct accgggccgg acgggccggc
     1021 gggctggccc ccgcgacgtt gaacgcggcc aacgaggtcg ccgtggaggc gttcctggcc
     1081 gggcggatcc gctggatcga catccctgac gttctctccg ccgtcctgga ccggcacaat
     1141 ggaggaaccg ccagcaccct cgacgacgtc ctcggtgccg atgccgacgc ccgtcggctg
     1201 gcgacccgtc tcgtcgagga gaggttcacc gcgtgaccag cctccagcac cccccgtcgc
     1261 agccggccca gccgccgacc ccaccgcccc cgcccggtcg caacccggac gaggagcgcg
     1321 gcaccagccc ggtccgcctg gccatcctcg ccggcctcgt cgcctggctc ggcatcgccg
     1381 gtgggtgggc gccgctcgtc gtcgtcttcg gcgtcgtggt catgatcttc ctccacgagc
     1441 tcggccacta cctggccgcc aagtggggcg ggatgaaggt caccgagttc ttcatcggct
     1501 tcggtcccag gatctggtcg ttccagcggg gcgagaccga gtacggactc aaggtcatcc
     1561 cggccggtgc ctacgtgaag gtcatcggca tgaacaacct cgacgaggtc gacccggccg
     1621 acgagggcaa gacctaccgc caggccagct acccccgccg catggccgtg gccgtggccg
     1681 gctccaccat gcacttcctg caggccatcc tcctgctgtt cgtcctgtac tccttcgtcg
     1741 gcgtccccgg cggcagcctc accgagga
//
LOCUS       DATISU010000221        13627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_243859, whole genome shotgun sequence.
ACCESSION   DATISU010000221 DATISU010000000
VERSION     DATISU010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 13627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13627
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243859"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /gene="cysC"
                     /locus_tag="VLR27_09075"
     CDS             complement(<1..504)
                     /gene="cysC"
                     /locus_tag="VLR27_09075"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HSP03639.1"
                     /translation="MTRATPNTVWHPSALDRTARPSLGATVWLTGLSGSGKSTVAVEV
                     ERLLVSTGRPAYLLDGDNLRQGLNGDLGFSADDRTENVRRVGEVARLFADAGVVALVP
                     VISPYRADRDRVRSIHELADLPFVEVFVDTPLEVCEARDPKGLYAKARAGEITGFTGI
                     DDPYEAPE"
     gene            complement(501..1754)
                     /locus_tag="VLR27_09080"
     CDS             complement(501..1754)
                     /locus_tag="VLR27_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSP03640.1"
                     /translation="MELLRFATAGSVDDGKSTLIGRLLYDSKTIFQDQLEAVERTSVE
                     RGDEYTNLALLTDGLRAEREQGITIDVAYRYFATPRRKFIIADTPGHIQYTRNMVTGA
                     STANVALVLVDARNGIVEQSRRHAFLASLLRIPHLVVCINKMDLVDWSEDRYEEIKAE
                     FRQFATKLDINDLTFIPVSALLGDNVVDRSDNMKWYEGLPLLRFLEEVHIASDRNLID
                     SRFPVQYVIRPMSSSGLDYRGYAGTVSGGVFKPGDEVVVLPSGFTSTIASIDGPNGPV
                     DEAYPPMSVTIRLADEIDISRGDMICRPKNQPIASQDVEAMVCWMSEVGSLQPRQKLA
                     IKHTTRWARAMVNDVQYRLDVNTLHRDEEASSLALNEIGRVSLRTTAPLFFDEYRRNR
                     FTGSFILVDEATNNTVAAGMILGES"
     gene            complement(1754..2659)
                     /gene="cysD"
                     /locus_tag="VLR27_09085"
     CDS             complement(1754..2659)
                     /gene="cysD"
                     /locus_tag="VLR27_09085"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HSP03641.1"
                     /translation="MSYELSHLRALEAEGIHIMREVAAEFERPVLLFSGGKDSIVMLE
                     LAIRAFAPAKIPFPVMHVDTGHNFPEVIEFRDSRLERAGARLIVASVQDSIDQGRVTE
                     IPGPRSSRNQLQTVTLLDAIEEHGFDAVFGGARRDEEKARAKERVFSFRDDFGQWDPK
                     GQRPEVWSLYNGRHRKGEHIRVFPISNWTELDVWQYLHEEDVEIPPIYFAHERKVFER
                     DGMLLSESPHIELLEGETVFTETVRYRTIGDMTCTGAVESQAATLEAIIEEVAATRIT
                     ERGATRADDRVSEAAMEDRKKEGYF"
     gene            2732..3505
                     /locus_tag="VLR27_09090"
     CDS             2732..3505
                     /locus_tag="VLR27_09090"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HSP03642.1"
                     /translation="MPARAAVSLADHELAKRLAVSAGELLVDLRGQMVADGADPKELK
                     DEGDRRANDLLLAELDQHMAPGDAVLSEEARATHDDGVRLGASRVWIIDPLDGTREFS
                     EPDRSDWAVHVALCVDGVPVCGAVALPALGTVLSTADPVLELTPIGQPRIVVSRTRPP
                     AEAETVRAALDGVLVEMGSAGAKTMAILRGEVDCYPHSGGQYEWDSAAPVAVALHHGL
                     HASRLDGSPLVYNRPDPYLPDLLVCRAELAERSLAALRD"
     gene            3510..4337
                     /locus_tag="VLR27_09095"
     CDS             3510..4337
                     /locus_tag="VLR27_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSP03643.1"
                     /translation="MDLKAIRYEVEDRVARLTLDRPERGNAWTGRMHLEYRWAITEAE
                     ADPDVRVIVVTGAAHPDGRTAFCVGADAKALEGHVDKGGYDDGLRGAEPPMPDVAAPF
                     AADFAFQLGMRTPVVAAVNGAAAGVGLVIACFADIRFGVQGAKLTTAAPKLGFPAEYG
                     LSWLLPRLVGAGRAADWLLSGRVFLTDEAAEVGLLSAVLPADELGPHVERYVTDLARH
                     VSPASVAATKAQLWGDLLHDDPAASVRRSMALLREMSTGPDFVEGSRALSERRPPEF"
     gene            4377..4688
                     /locus_tag="VLR27_09100"
     CDS             4377..4688
                     /locus_tag="VLR27_09100"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HSP03644.1"
                     /translation="MAAKDRDRDDSEVVNRYSGTGVTPTVIALALLAIAVVILLAQNT
                     ADVPFRFVTFEADVPLYVLFLVTALAASLMTVLASAIWRRRRRRARNEHEELERLRQR
                     G"
     gene            complement(4701..5867)
                     /locus_tag="VLR27_09105"
     CDS             complement(4701..5867)
                     /locus_tag="VLR27_09105"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSP03645.1"
                     /translation="MTVLEGIRVVELGAWVAGPGAGGLLADWGADVIKVEAPEGDPMR
                     RLFAVLSGHGEPQSPPFDLDNRGKRSVVLDLRSEDGRRDLHDLVATADVFLTNLRPDA
                     VERLGFGPDVLLEANDRLVYAQVSGFGRTGPDAHRAGYDLGAFFARSGMASQAVPPDQ
                     APVANAGGVGDHITGTTITAGICAALFARERSGRGQLVDTSLLRAGIYTMGWPLNIQL
                     RYGKHLPTDPRTTVGNPLINSYEAGDGRWFWLLGLEADRHWPPLLRAIGRPELADDER
                     FGSAKARRINAPALIALLDETFATADRDHWTARFDEHDVWWAPVNTLAEVVEDPQAVA
                     SGAFVDVPAGKGSAAHRAVMTPVDFGSTPAGPRRGVPGLGEHTDEVLAEVRQPR"
     gene            5929..7533
                     /locus_tag="VLR27_09110"
     CDS             5929..7533
                     /locus_tag="VLR27_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HSP03646.1"
                     /translation="MASPDPTELPADHELGSFSDTPPWIIERDRLTWLPPLARVRREV
                     RNEIPRLTRPHWIPPVRRVTTVTRHLGWAVGRWYVGKRRTGGSISRADLSRRLRIAAE
                     ALGPTYIKLAQIISSGEGLFPEELVAETKKCRDQVPPESWEEIRAVVEAELGLPIHEL
                     FSHFDTEPLAAASIAQVHRATLHTGEDVVVKVQRPSVAFRVKEDLRVMAWLAPKLIGR
                     IPVSALANPPALVELFAETISEELDFRLEAENMLDVAASFAELGQRDYVIPRPHPELV
                     TPRVLVMERLDGFKFDDVAGMKGAGVDTAQVVRTGMIGFMEGALIHGIFHGDLHGGNL
                     FVMPDGRTALLDFGITGRLDDFKRNAFLRLLMSGTSNDPIGQLAAFRDLGALPPDTDL
                     EAVARDLGLDSPEVFDPTKVDGDALVAEIQRVIKALLGYGAKIPKELMLFTKNLMFVD
                     GAIATLAPDLDILEEIAHITMYFATTHGEKIAAQIGMEPDAWELDMEGVKAGFGISDD
                     VERLTYRDLQQRRETIRKRLEGRSLQ"
     gene            7536..8840
                     /locus_tag="VLR27_09115"
     CDS             7536..8840
                     /locus_tag="VLR27_09115"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HSP03647.1"
                     /translation="MPGNRLDEVAPGVLVATSRFMATNTVVLTHGRDALLVDPGVHRD
                     ELESLAGELLARRLEPVGGFATHAHWDHVLWHRDLGEVPRWASAATVTDGIAHHHELV
                     DQAEAVVELDDERLGLSLTPVTGALPWSGPEVVPVTHDAHATGHSALHVPELGLLVAG
                     DMGSDIEVPLLEHGVPGPQALLAYHEGLERLAALTPVDLVVTGHGHVCDGAMWRRRLD
                     ADRRYLDDLAAGRPTDDTRLVEPWLVDADAGMRASLTKRRWREWARTLPSPGGSETAG
                     VRAGLADFLGTRPGFVAAYLPLPGEVDLSGVLVGLGADVVALPRMEDDRTVSWRSDDG
                     DRERNDLGFDQPADHLPWVDPFDFDVVLVPGRLFDRHGVRLGRGGGHYDRLLPRLRPG
                     AAVVGVTVDERIVPRLPTEHHDRPMTHLATESGVRAVTGPRT"
     gene            8852..9526
                     /gene="nucS"
                     /locus_tag="VLR27_09120"
     CDS             8852..9526
                     /gene="nucS"
                     /locus_tag="VLR27_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HSP03648.1"
                     /translation="MRLVIARCSVAYRGKLAAHLPSAQRLILVKADGSISIHSDDRAY
                     KPLNWMNPPCSITESTDDDGLPHWLVRGKGGDELEITIEDLVSDSAHDLGPEPGLQKD
                     GVEAHLQALLAERCEVIGEGFQLVRREYPTDIGPVDLLCRDTEGIAVAVEVKRRGEID
                     GVEQLTRYLGFLNKDPRLAPVRGVFVAEQIKPQAKVLAADRGIAWVEIDYDELRGIES
                     SELRLF"
     gene            9528..9830
                     /locus_tag="VLR27_09125"
     CDS             9528..9830
                     /locus_tag="VLR27_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03649.1"
                     /translation="MGARSQVEVVHQPWPVVYDALLRCLPSAGFTITGADPDRGRIDL
                     ETRNTRLTVAVGAIDAISSEWMATSELKLGLFRDRHEQKFAAIRDALERYLAAYYA"
     gene            complement(9843..10334)
                     /locus_tag="VLR27_09130"
     CDS             complement(9843..10334)
                     /locus_tag="VLR27_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03650.1"
                     /translation="MVRPLAIVDLDGVVADVRHRLVHLTGRRKDWDAFFAAAVDDPPH
                     PEGIAVVERLRQDHDVVYLTGRPERCRADTEAWLERNGIGGPPLHMRSGRDRRPAAVV
                     KPELLRTIAQGREVAVVVDDDERVVEAMRRAGYQVLHADWEKRAAEEEAALERAQEEQ
                     GRT"
     gene            complement(10379..11719)
                     /gene="gdhA"
                     /locus_tag="VLR27_09135"
     CDS             complement(10379..11719)
                     /gene="gdhA"
                     /locus_tag="VLR27_09135"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HSP03651.1"
                     /translation="MIDEKLENVISEVIARNPGQTEFHQSVREVLESLGPVVAKHPEF
                     LDHKVIERICEPERQILFRVPWQDDRGEVQINRGMRVEFNSALGPFKGGLRFHPSVNL
                     GIVKFLGFEQIFKNALTGMPIGGGKGGANFDPKGRSDSEIMRFCQSFMTELYRHLGEY
                     TDVPAGDIGVGGREIGYLFGQYKRITNRYESGVLTGKGIEWGGALVRTEATGYGAVLF
                     AQEMLKARGESLDGRTCVVSGSGNVAIYAIEKAHQLGGTVVACSDSGGVVHDPKGIDF
                     DLLKQIKEVERGRVSQYAERRGSATYTDRGNIWDIPCDVAFPSATQNELTGRDAKALV
                     SNGCIAVSEGANMPSTPEAVDVFQEAGIAFGPGKAANAGGVATSALEMQQNASRDSWS
                     FSYTEDRLAEIMRSIHDTCFATAEEYGAPGNYVTGANIAGFLKVATAMDALGLV"
     gene            complement(11801..12652)
                     /locus_tag="VLR27_09140"
     CDS             complement(11801..12652)
                     /locus_tag="VLR27_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HSP03652.1"
                     /translation="MPINPDAVGVKSEPTRQSWTSKDALLYAVGVGAGQEPLEELQYT
                     TENSADIDQKVLPTMAVVLGGGGGAFAQIGSFNPAMLVHGEQQISLHREIPVEGEIET
                     VSEITGIYDKGKGAVVVSEATSTLVSDGQPLFSKTMSAFIRGEGGWGGDSGPALEWER
                     PDGDPDEVVTYTTRPEQALIYRLSGDRNPLHADPKFAAMGGFDRPILHGLCTYGFTGR
                     GLLTALCDGDSSRFRSMYGRFSSPVLPGEDLTVKVWRTGDGEAVYQTEGGDGRVVLDK
                     GTCSFDG"
     gene            complement(12655..13617)
                     /locus_tag="VLR27_09145"
     CDS             complement(12655..13617)
                     /locus_tag="VLR27_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSP03653.1"
                     /translation="MVTRPSTENRRGDEKGRTHMSGICDGRVAIVTGAGRGIGREHSL
                     MLAEQGAKVVVNDLGGSMDGTGDDAGPAQQVVDEITAMGGEAVANTDDISSWDGAERL
                     INQAVEHFGRLDVLINNAGILRDRMLTNMTEAEWDAVIQVHLKGTFAPSRHAAAYWRE
                     QSKAGNEVDGRIINTSSPSGIYGNVGQTNYGAAKAGIASFTIIAAKELGRYGVTVNAI
                     APAALTRMTENLGMGQLPEEQKERMSPRYIAPIVVWLASADSKDVTGRVFDVSGRALS
                     VSEGWHRGPTIELPSDDPAALADDVKRIVSEARPNANMAGYDEE"
BASE COUNT         1867 a         4948 c         4765 g         2047 t
ORIGIN      
        1 ctccggcgcc tcgtaggggt cgtcgatccc cgtgaagccg gtgatctccc cggcccgggc
       61 cttggcgtag aggcccttgg ggtcccgtgc ctcgcacacc tccagcggcg tgtcgacgaa
      121 gacctccacg aacggcaggt ccgccagctc gtggatcgag cggacgcggt cgcggtcggc
      181 gcggtacggc gagatgacgg gcacgagggc gacgacgccg gcatcggcga acaggcgggc
      241 gacctcgccg acccgccgca cgttctcggt gcggtcgtcg gcgctgaacc cgaggtcccc
      301 gttcaggccc tggcgcaggt tgtcgccgtc gagcaggtac gccgggcgac cggtggacac
      361 gagcagccgc tcgacctcca ccgccacggt cgacttgccg gatccggaca gtccggtgag
      421 ccacacggtg gcgccgagcg agggtcgggc ggtccggtcc agcgcggagg ggtgccagac
      481 cgtgttcggg gtggcgcggg tcaactttcc ccgaggatca tcccggcggc caccgtgttg
      541 ttggtggcct cgtccacgag gatgaagctg ccggtgaagc ggttgcggcg gtactcgtcg
      601 aagaacagcg gcgcggtggt gcgcagcgag acccggccga tctcgttcag ggccaggctc
      661 gacgcctcct cgtcccggtg cagggtgttc acgtcgaggc ggtactgcac gtcgttcacc
      721 atggcgcgcg cccagcgggt ggtgtgcttg atggccagct tctggcgggg ctggagcgag
      781 ccgacctcgg acatccagca caccatggcc tccacgtcct gggacgcgat cggctggttc
      841 ttgggacggc agatcatgtc gccgcggctg atgtcgatct cgtcggcgag gcggatcgtg
      901 accgacatcg gcgggtaggc ctcgtcgacg ggtccgttgg ggccgtcgat cgacgcgatg
      961 gtcgaggtga accccgaggg cagcaccacg acctcgtcgc ccggcttgaa cacgccaccg
     1021 gacacggtgc cggcgtagcc ccggtagtcg aggcccgacg agctcatcgg gcggatcacg
     1081 tactggaccg ggaagcggct gtcgatgagg ttgcggtccg acgcgatgtg gacctcctcg
     1141 aggaagcgca gcagcggcag gccctcgtac cacttcatgt tgtcggaccg gtcgaccacg
     1201 ttgtcgccca gcagcgccga gaccgggatg aaggtcaggt cgttgatgtc gagcttggtg
     1261 gcgaactggc ggaactcggc cttgatctcc tcgtagcggt cctcgctcca gtcgaccagg
     1321 tccatcttgt tgatgcacac gacgaggtgc gggatccgca gcagcgaggc gaggaaggcg
     1381 tggcggcggg actgctcgac gatgccgttg cgggcatcca ccagcaccag ggccacgttg
     1441 gccgtcgagg cacccgtgac catgttgcgc gtgtactgga tgtggcccgg ggtgtcggcg
     1501 atgatgaact tgcgccgggg ggtggcgaag taccggtagg cgacgtcgat ggtgatgccc
     1561 tgctcgcgct cggcccgcag accgtcggtg agcagcgcca ggttcgtgta ctcgtcgccg
     1621 cgctcgaccg aggtgcgctc gaccgcctcc agctggtcct ggaagatggt cttggagtcg
     1681 tacagcagcc gcccgatgag ggtggacttg ccgtcgtcga ccgagccggc cgtggcgaac
     1741 cgcaggagct ccatcagaaa tagccctcct tcttgcggtc ctccatggcg gcctcgctca
     1801 cccggtcgtc ggcgcgggtg gcgcctcgct cggtgatgcg ggtcgcggcg acctcttcga
     1861 tgatggcctc caacgtggcg gcctgggact cgacggcgcc ggtgcaggtc atgtcaccga
     1921 tcgtgcggta gcgcaccgtc tcggtgaaca cggtctcgcc ctcgagcagc tcgatgtgcg
     1981 gggactccga cagcagcatc ccgtcgcgct cgaacacctt gcgctcgtgc gcgaagtaga
     2041 tcggcgggat ctcgacgtcc tcctcgtgca ggtactgcca cacgtcgagc tcggtccagt
     2101 tgctgatcgg gaacacccgg atgtgctcgc ccttgcggtg ccggccgttg tacaggctcc
     2161 acacctcggg acgctggccc ttcgggtccc actgaccgaa gtcgtcgcgg aaggagaaca
     2221 cccgctcctt ggcccgggcc ttctcctcgt cgcgccgggc gccgccgaac acggcgtcga
     2281 agccgtgctc ctcgatcgcg tcgagcagcg tgaccgtctg cagctggttg cggctcgacc
     2341 gaggacccgg gatctcggtg acccggccct ggtcgatcga gtcctgcacc gacgccacga
     2401 tcaggcgggc accggcccgc tcgaggcggc tgtcgcggaa ctcgatcacc tcggggaagt
     2461 tgtgcccggt gtcgacgtgc atcaccggga acgggatctt cgccggcgcg aacgcccgga
     2521 tcgccagctc gagcatgacg atggagtcct taccacccga gaacagcagc accggccgct
     2581 cgaactcggc cgccacctcg cgcatgatgt ggatcccctc ggcctcgagg gctcggaggt
     2641 gggacagctc gtagctcatc gggtcctgct cgtggggtcc ggacgtgagt gggcaaatgt
     2701 acggtcggcg ccggttggta gacaatccag cgtgccagcc cgcgccgccg tctccctcgc
     2761 cgatcacgaa ttggccaagc ggctggcggt gtcggccggc gagctgctcg tcgacctgcg
     2821 cggccagatg gtggccgacg gggccgaccc gaaggagctg aaggacgagg gcgaccgccg
     2881 cgccaacgac ctgctgctgg ccgagctcga ccagcacatg gcccccggcg acgccgtgct
     2941 gtcggaggag gcccgagcca cccacgacga cggggtccgc ctcggtgcct cccgcgtctg
     3001 gatcatcgac ccgctcgacg gcacccgcga gttctccgag cccgaccgca gcgactgggc
     3061 ggtgcacgtg gccctgtgcg tcgacggcgt cccggtctgc ggggcggtcg cgctgccggc
     3121 gctcggcacc gtgctctcca ccgccgatcc ggtgctcgag ctgacaccca tcggccaacc
     3181 ccgcatcgtg gtgagccgca cccggccacc ggccgaggcc gagaccgtgc gcgccgcgct
     3241 cgatggcgtg ctggtcgaga tgggctcggc cggggccaag acgatggcga tcctgcgcgg
     3301 cgaggtcgac tgctaccccc actccggcgg gcagtacgag tgggactccg ccgcgccggt
     3361 cgccgtggcc ctgcaccacg gcctgcacgc cagtcgcctc gacgggtcgc ccctcgtcta
     3421 caaccggccc gacccctatc tccccgacct gctcgtgtgc cgggccgagc tggccgagcg
     3481 ctccctcgct gcgctccggg actgagccgg tggacctcaa ggccatccgc tacgaggtcg
     3541 aggaccgggt cgcccggctc accctcgacc ggcccgagcg cggcaacgcg tggacgggcc
     3601 gcatgcacct cgagtaccgc tgggcgatta ccgaggccga ggccgacccc gacgtccggg
     3661 tgatcgtggt gaccggggca gcgcacccgg acggccgcac cgcgttctgc gtgggcgccg
     3721 acgccaaggc gctggagggc cacgtcgaca agggcggcta cgacgacggc ctgcgcgggg
     3781 ccgagccgcc catgccagac gtcgccgccc cgttcgccgc cgacttcgcc ttccagctcg
     3841 gcatgcggac accggtggtc gcagcggtca acggcgccgc ggccggcgtc ggcctcgtca
     3901 tcgcctgctt cgccgacatc cgcttcgggg tccagggcgc caagctcacg accgcggccc
     3961 ccaagctggg cttcccggcc gagtacgggc tgtcttggct gctcccccgc ctggtgggcg
     4021 ccggccgggc cgccgactgg ctcctgtccg gccgggtgtt cctcaccgac gaggccgcag
     4081 aggtcgggct cctgtcggcg gtgctgccgg cggacgagct cggcccgcac gtcgagcgct
     4141 acgtcaccga cctggcccgc cacgtgtccc cggcgtccgt cgccgccacc aaggcccagc
     4201 tctgggggga cctgctgcac gacgaccccg ccgcgtcggt ccggcgttcg atggccctgc
     4261 tgcgggagat gtcgaccggg cccgacttcg tcgagggcag ccgcgcgctg tccgagcggc
     4321 gaccgccgga gttctgactc cttctatgac gcttcacccg ggggtacgag gctgacatgg
     4381 ccgccaagga ccgagaccgc gacgactccg aggtcgtcaa ccgctactcc ggcaccggcg
     4441 tcacccccac ggtgatcgcc ctggcgctgc tcgccatcgc cgtggtgatc ctgctggccc
     4501 agaacacggc ggacgtgccg ttccggttcg tgaccttcga ggccgacgtc cccctctacg
     4561 tcctgttcct ggtgacggcg ctcgctgcgt cgctgatgac cgtgctggcg tcggccatct
     4621 ggcggcgtcg ccggcgccgc gccaggaacg agcacgagga gctggagcga ctgcgccagc
     4681 gcggctgacg cacctcggcg tcagcgcggc tgacgcacct cggccagcac ctcatcggtg
     4741 tgctccccca accccgggac gccgcgacgt gggccggccg gcgtcgaccc gaagtccacc
     4801 ggtgtcatca cggcccggtg cgccgctgac cccttccccg ccgggacgtc cacgaacgcg
     4861 ccggacgcca cggcctgggg gtcctccacg acctcggcga gggtgttcac cggcgcccac
     4921 cacacgtcgt gctcgtcgaa gcgggccgtc cagtggtcgc gatcggcggt ggcgaaggtc
     4981 tcgtccagga gggcgatcag cgccggtgcg ttgatgcgtc gggccttcgc cgacccgaac
     5041 cgctcgtcgt ccgccagctc cggtcgcccg atggcccgca gcagcggcgg ccagtgccgg
     5101 tcggcctcga ggccgagcag ccagaaccac cggccgtccc cggcctcgta ggagttgatc
     5161 agcgggttgc cgaccgtggt gcgcgggtcg gtgggcaggt gcttgccgta ccggagctgg
     5221 atgttgagcg gccagcccat cgtgtagatc ccggcccgca gcagcgaggt gtcgacgagc
     5281 tggccccgac cggaccgctc ccgggcgaac agggcggcgc agatgccggc ggtgatcgtg
     5341 gtgccggtga tgtggtcgcc gacgcccccg gcgttggcca ccggcgcctg gtcgggcggc
     5401 accgcctggc tcgccatgcc cgaccgggcg aagaacgcgc ccaggtcgta gccggcccgg
     5461 tgggcgtccg ggccggtgcg cccgaacccg gagacctgtg cgtagacgag tcggtcgttc
     5521 gcctcgagca ggacgtcggg gccgaacccg aggcgctcga ccgcgtccgg ccggaggttg
     5581 gtgaggaaca cgtcggccgt ggcgacgagg tcgtgcaggt cccggcggcc gtcctcgctg
     5641 cgcaggtcga gcacgacgga gcgcttgccg cggttgtcca ggtcgaaggg gggcgactgc
     5701 ggctcgccgt gcccggacag cacggcgaag agccgccgca tcgggtcgcc ctcgggcgcc
     5761 tccaccttga tgacgtcggc tccccagtcg gccagcaggc cgccggcgcc cgggccggcc
     5821 acccacgccc ccagctcgac gacgcggatc ccctcgagaa cggtcatggt cgcatcctgc
     5881 ctgctggggc gggtgtggag cggaccgggt ccctgcgatg atggtccgat ggcgtcgcct
     5941 gatccgaccg agctccccgc cgatcacgag ctcgggtcgt tctccgacac cccaccgtgg
     6001 atcatcgagc gcgaccggct cacctggctg cccccgctcg cccgcgtccg gcgcgaggtc
     6061 cgcaacgaga tcccccgcct cacccggccg cactggatcc caccggtccg gcgggtcacc
     6121 accgtcaccc gccacctcgg ctgggccgtc gggcgctggt acgtcggcaa gcggcggacc
     6181 ggcggctcga tctcccgggc cgatctgtcc cggcgactcc gcatcgccgc cgaggcgctc
     6241 ggacccacct acatcaagct cgcccagatc atctccagcg gcgagggcct gttccccgag
     6301 gagctggtcg ccgagaccaa gaagtgccgc gaccaggtgc cgccggagtc gtgggaggag
     6361 atccgggccg tggtcgaggc cgagctcggc ctgccgatcc acgagctgtt cagccacttc
     6421 gacaccgagc cgctcgcggc tgcctccatc gcccaggtcc accgggccac gctgcacacc
     6481 ggcgaggacg tcgtggtcaa ggtccagcgg ccgtccgtgg ccttccgggt caaggaggac
     6541 ctccgggtca tggcctggct ggcgccgaag ctgatcgggc gcatcccggt gtcggcgctc
     6601 gccaacccgc cggcgctggt ggagctgttc gccgagacca tcagcgagga gctcgacttc
     6661 cgactcgagg ccgagaacat gctcgacgtc gcggccagct tcgccgagct gggccagcgc
     6721 gactacgtca tcccccgccc ccaccccgag ctggtcacgc cccgggtcct ggtcatggag
     6781 cgactcgacg gcttcaagtt cgacgacgtc gccggcatga agggcgccgg cgtcgacacc
     6841 gcccaggtgg tccgcaccgg gatgatcggc ttcatggagg gcgcgctgat ccacggcatc
     6901 ttccacggcg acctccacgg cgggaacctg ttcgtgatgc ccgacggccg cacggcgctg
     6961 ctcgacttcg gcatcaccgg ccgcctcgac gacttcaagc gcaacgcctt cctgcgcctg
     7021 ctcatgtcgg gcacgtcgaa cgacccgatc ggccagctcg ccgccttccg cgacctcggc
     7081 gcgctgcccc ccgacaccga cctcgaggcg gtggcccggg acctgggcct cgacagcccc
     7141 gaggtgttcg acccgaccaa ggtcgacggc gatgcgctcg tcgccgagat ccagcgggtg
     7201 atcaaggcgc tgctgggcta cggcgccaag atccccaagg agctcatgct cttcaccaag
     7261 aacctgatgt tcgtcgacgg cgccatcgcc acgctcgccc cggacctgga catcctcgag
     7321 gagatcgccc acatcacgat gtacttcgcc accacgcacg gcgagaagat cgcggcgcag
     7381 atcggcatgg aacccgacgc gtgggagctg gacatggagg gcgtgaaggc gggcttcggc
     7441 atctccgacg acgtggagcg cctcacgtac cgggacctcc agcagcgacg cgagaccatc
     7501 cggaagcggc tcgagggtcg cagcctccag tagcggtgcc cgggaaccgc ctggacgagg
     7561 tcgcgcccgg cgtcctcgtc gccaccagcc gattcatggc gaccaacacc gtcgtgctca
     7621 cccacggccg ggacgcgctc ctcgtggacc ccggcgtcca ccgcgacgag ctcgagtccc
     7681 tggccggcga gctgctcgcc cggcggctcg aacccgtcgg cggcttcgcc acccacgcgc
     7741 actgggacca cgtgctctgg caccgcgacc tcggcgaggt gccccgctgg gcgtcggccg
     7801 cgacggtcac cgacggcatc gcccaccacc acgagctggt cgaccaggcc gaggccgtgg
     7861 tcgagctcga cgacgagcgc ctcggcctgt cgctcacgcc ggtgaccggc gcgctgccgt
     7921 ggagcggccc cgaggtcgta cccgtcaccc acgacgccca cgccaccggc cacagcgccc
     7981 tccacgtccc cgagctcggc ctgctcgtcg ccggggacat gggcagcgac atcgaggtgc
     8041 cgctgctgga gcacggcgtc cccggcccgc aggccctgct cgcctaccac gaggggctcg
     8101 agcggctcgc ggccctgacc ccggtggacc tcgtcgtcac cggtcacggg cacgtgtgcg
     8161 acggtgccat gtggcgccgc cgcctcgacg ccgatcgccg ctacctcgac gacctcgccg
     8221 ccggccggcc caccgacgac acccgcctgg tcgaaccgtg gctcgtggac gccgacgccg
     8281 gcatgcgagc gtcgctcacc aagcgccgtt ggcgggagtg ggcccgcacg ctcccatccc
     8341 ccggaggttc ggagaccgct ggcgtccgcg ccggtctcgc cgacttcctc ggcacccgac
     8401 ccggcttcgt cgccgcctac ctcccgctcc ccggtgaggt cgatctctcc ggcgtgctgg
     8461 tcgggctcgg tgccgacgtc gtggccctcc cccgcatgga ggacgaccgc accgtcagct
     8521 ggcgcagcga cgacggcgac cgcgagcgca acgacctcgg cttcgaccag cctgcggacc
     8581 acctcccgtg ggtcgatccc ttcgacttcg acgtcgtgct cgtccccggg cggctcttcg
     8641 accgccacgg ggtccgcctc ggccggggtg ggggccacta cgaccggctc ctcccccgcc
     8701 tgcgccccgg cgccgcggtc gtcggcgtca ccgtggacga gcgcatcgtc ccccggctgc
     8761 ccaccgagca ccacgaccgg cccatgaccc acctcgccac cgaatccggt gttcgcgccg
     8821 tgacggggcc ccgcacctag cctcgaccgt cgtgcgcctc gtcatcgccc gctgctccgt
     8881 cgcctaccgc ggcaagctcg ccgcccacct cccgagcgcc cagcgcctca tcctggtgaa
     8941 ggccgacggc tcgatctcca tccactccga cgaccgggcc tacaagcccc tcaactggat
     9001 gaacccgccg tgctcgatca ccgagtcgac cgacgacgac ggcctgccgc actggctggt
     9061 gcggggcaag ggcggcgacg agctcgagat caccatcgag gacctggtca gcgactcggc
     9121 ccacgacctc ggccccgagc ccgggctgca gaaggacggc gtcgaggccc acctgcaggc
     9181 cctgctcgcc gagcgctgcg aggtcatcgg cgaggggttc cagctggtgc ggcgcgagta
     9241 ccccaccgac atcggcccgg tcgacctgct gtgccgggac accgagggca tcgccgtcgc
     9301 ggtcgaggtg aagcgccggg gtgagatcga cggcgtcgag cagctcaccc gctacctggg
     9361 cttcctgaac aaggaccccc ggctggcacc ggtgcgcggg gtgttcgtgg ccgagcagat
     9421 caagccgcag gccaaggtgc tggcggccga ccgcggcatc gcctgggtcg agatcgacta
     9481 cgacgagctg cgcggcatcg agtcgtccga gctgcggctg ttctgacgtg ggcgcccgct
     9541 cccaggtcga ggtcgtccac cagccctggc cggtcgtcta cgacgccctc ctgcgctgcc
     9601 tgcccagcgc cggcttcacg atcaccgggg ccgatcccga ccgcggccgc atcgacctcg
     9661 agacccggaa cacgcggctc accgtcgccg tcggcgcgat cgacgcgatc agcagcgagt
     9721 ggatggccac gtcggagctg aagctgggac tgttccgcga ccgccacgag cagaaattcg
     9781 ccgccatccg cgacgcgctc gagcgctacc tcgccgccta ctacgcctga acccagttcg
     9841 gttcaggtgc ggccctgctc ctcctgggcc cgctcgagcg ctgcctcctc ctcggcggcc
     9901 cgcttctccc agtcggcgtg gagcacctgg tagccggccc ggcgcatcgc ctcgacgacc
     9961 cgctcgtcgt cgtcgaccac gaccgccacc tcccgaccct gcgcgatcgt gcgaagcagc
    10021 tcgggcttca ccaccgcggc cggcctccga tcacgaccgg agcgcatgtg cagcgggggg
    10081 ccgccgatgc cgttccgttc gagccaggcc tcggtgtcgg cccggcagcg ctcgggccga
    10141 ccggtcaggt acacgacgtc gtggtcctgc cgcaggcgct cgaccaccgc gatgccctca
    10201 gggtggggcg ggtcgtcgac ggccgcggcg aagaacgcgt cccagtcctt gcgccggccc
    10261 gtcaggtgga ccaggcggtg gcggacgtcg gcgacgacgc cgtcgaggtc gacgatggcg
    10321 agcggtcgga ccatgacccg tccgtaccct cagctcgtcg ggtcaccgtg gatgacggtc
    10381 acaccaggcc gagcgcgtcc atcgccgtgg cgaccttgag gaagccggcg atgttggcgc
    10441 ccgtgacgta gttgcccggc gcgccgtact cctcggcggt ggcgaagcag gtgtcgtgga
    10501 tcgagcgcat gatctcggcc aggcggtcct cggtgtagga gaagctccac gagtcccggc
    10561 tggcgttctg ctgcatctcg agcgccgagg tggccacgcc gccggcgttg gccgccttgc
    10621 ccggcccgaa ggcgatgccg gcctcctgga acacgtccac ggcctcgggc gtcgagggca
    10681 tgttggcgcc ctccgacacg gcgatgcagc cgttggagac gagggccttg gcgtcgcggc
    10741 cggtgagctc gttctgcgtc gccgacggga aggccacgtc gcaggggatg tcccagatgt
    10801 tgccccggtc ggtgtaggtg gcagaaccac ggcgctcggc gtactgactc acccggccgc
    10861 gctcgacctc cttgatctgc ttgagcaggt cgaagtcgat gcccttgggg tcgtggacga
    10921 ccccacccga gtcggagcag gcgacgacgg tgccgccgag ctggtgcgcc ttctcgatgg
    10981 cgtagatggc gacgttgccc gaaccggaca ccacgcaggt gcgcccgtcg agcgactcgc
    11041 cgcgggcctt cagcatctcc tgggcgaaca gcaccgcgcc gtagcccgtg gcctcggtgc
    11101 gcacgagcgc cccgccccat tcgatgccct tgccggtgag gacgccggac tcgtatcggt
    11161 tcgtgatccg cttgtactgg ccgaacaggt agccgatctc gcggccgccg acgccgatgt
    11221 cgccggccgg cacgtcggtg tactcgccca ggtggcggta cagctcggtc atgaagctct
    11281 ggcagaaccg catgatctcg ctgtcggagc gacccttcgg atcgaagttc gcaccgccct
    11341 tgccgccgcc gatgggcatg ccggtgaggg cgttcttgaa gatctgctcg aagccgagga
    11401 acttgacgat gccgaggttc accgacgggt ggaaccgcag gccgcccttg aacggaccga
    11461 gggcgctgtt gaactccacc cgcatgccac ggttgatctg gacctcgccc cggtcgtcct
    11521 gccacggcac gcggaacagg atctggcgct ccggctcgca gatgcgctcg atgaccttgt
    11581 ggtcgaggaa ctcggggtgc ttcgcgacga cggggccgag ggactcgagc acctcgcgga
    11641 cgctctggtg gaactcggtc tggcccgggt tgcgggcgat gacctcgctg atgacgttct
    11701 cgagcttctc gtcgatcacg gtgcgctcct cggcgatcgg tgcaggcagg ggggacccca
    11761 ggctacggag cacccctgcc tgaaccgaat ctgacccgag tcagccgtcg aagctgcagg
    11821 tccccttgtc gagcaccacg cgaccgtcgc cgccctcggt ctggtagacg gcctccccgt
    11881 cgccggtgcg ccacaccttc acggtgaggt cctcgccggg gaggaccggg gacgagaagc
    11941 ggccgtacat ggagcggaac cgggacgagt cgccgtcgca gagggccgtg agcaggccgc
    12001 ggccggtgaa gccgtaggtg cacaggccgt gcaggatcgg tcggtcgaag ccgcccatcg
    12061 ccgcgaactt cgggtcggcg tggagcgggt tgcggtcgcc ggacaggcgg tagatgagcg
    12121 cctgctcggg acgggtggtg taggtcacga cctcgtcggg gtcgccgtcg gggcgctccc
    12181 actcgagcgc gggaccggag tcgccgcccc agccgccctc accgcggatg aaggccgaca
    12241 tggtcttgga gaacaggggt tggccgtcgg agaccagcgt cgaggtggcc tcgctgacca
    12301 ccacggcacc cttgcccttg tcgtagatgc cggtgatctc gctcaccgtc tcgatctcgc
    12361 cctcgaccgg gatctcccgg tgcagggaga tctgctgctc gccgtggacc agcatcgccg
    12421 ggttgaagct gccgatctgg gcgaaggcgc caccgccgcc gccgaggacg accgccatgg
    12481 tgggcagcac cttctggtcg atgtcggcgg agttctcggt ggtgtactgc agctcctcga
    12541 gcggctcctg cccggcgccg acgccgacgg cgtagagcag ggcgtccttg gaggtccagg
    12601 actggcgagt cggttcgctc ttgacgccga cggcatcggg gttgatgggc atccctactc
    12661 ctcgtcgtag ccggccatgt tggcgttcgg ccgggcctcg gagacgatgc gcttgacgtc
    12721 gtcggccagg gcggccgggt cgtcgctggg cagctcgatg gtggggccgc ggtgccagcc
    12781 ctcggacacc gacagggccc gaccggacac gtcgaagacc cggccggtga cgtccttgga
    12841 gtcggcgctg gcgagccaca ccacgatcgg ggcgatgtag cggggcgaca tgcgctcctt
    12901 ctgctcctcc ggcagctggc ccatgccgag gttctcggtc atgcgggtga gcgcggcggg
    12961 ggcgatggcg ttgaccgtga ccccgtagcg gccgagctcc ttggcggcga tgatcgtgaa
    13021 gctggcgatg ccggccttgg ccgcgccgta gttggtctgg cccacgttgc cgtagatgcc
    13081 cgacggcgag gaggtgttga tgatgcgccc gtcgacctcg ttgccggcct tggactgctc
    13141 gcgccagtag gcggcggcgt ggcggctcgg tgcgaacgtg cccttgaggt gcacctggat
    13201 gaccgcgtcc cactcggcct cggtcatgtt ggtgagcatc cggtcccgga ggatgccggc
    13261 gttgttgatc agcacgtcga ggcggccgaa gtgctccacc gcctggttga tcaggcgctc
    13321 ggcaccgtcc cagctggaga tgtcgtcggt gttggcgacg gcctcgccgc ccatggccgt
    13381 gatctcgtcg acgacctgct gggccggacc ggcgtcgtca ccggtgccgt ccatcgagcc
    13441 gccgaggtcg ttcacgacga ccttggctcc ctgctcggcg agcatgaggg agtgctcgcg
    13501 gccgatcccc cgtccggctc cggtcacgat cgcgacccgt ccgtcacaga tgccactcat
    13561 gtgtgtgcgt cccttctcgt ctcctcgccg gttctcggtc gagggccgcg tgaccatagg
    13621 cacgcga
//
LOCUS       DATISU010000222         8191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_197844, whole genome shotgun sequence.
ACCESSION   DATISU010000222 DATISU010000000
VERSION     DATISU010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8191
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197844"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VLR27_09150"
     CDS             <1..571
                     /locus_tag="VLR27_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03654.1"
                     /translation="ACVVHFDPPEDDKTYLHRSGRTARAGAQGVVVSFVRDNNRKDVK
                     TLQRDLGLAQVVTTPDSTLLGDGSRHREATSRQKSQPVAKTQQSSPPKGGGGNGGRNR
                     QRTRTRDGEDRAAGKSARRDDRPARDGGKPRTRTKPAERRGADGVVDVDPRPRKERQG
                     NGGRTNPPSKVHRKGGGAKRQAIEASRPR"
     gene            complement(590..1615)
                     /locus_tag="VLR27_09155"
     CDS             complement(590..1615)
                     /locus_tag="VLR27_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HSP03655.1"
                     /translation="MARLADQPPLAVVGERLCTDLTDVTDDPACLDGEGFWAVVVPYD
                     GPPTFARFATVRPARPWRGPRWVGPDRDAWTTSLDRDAFEEGVRTIRACIEAGDVYQV
                     NLTRQLAAPVDPDADVAALGAALAVGNPAPYAAVVRVPAAGIHVASASPERFLRREGR
                     LVWSSPIKGTAATPDAFLAKDRAENVMIVDLVRNDLGRVCDFGSVCVPALLAVEHHPG
                     LVHLVSTVAGELCDGVGWPQLLDATFPPGSITGAPKLAAMEVIRQLEPVPRGVYCGGV
                     GWVDADRGIGELNVAIRTFWIDPEGTRGPTLRFGTGGGITHDSDPAGEWAETELKART
                     LLAVASG"
     gene            1675..3126
                     /locus_tag="VLR27_09160"
     CDS             1675..3126
                     /locus_tag="VLR27_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HSP03656.1"
                     /translation="MSGTETRRARRSVLQKLVIITNVFAMVGFLFAAGSLAYGYEKFG
                     QIPRVEIGETLSVEDADDGREVIAENFLIVGVDSAAGLDPDDPVAASRTDIANLRSDT
                     MMILRVDPTSTRAALLSLPRDLWVPIPGHGNGRINEAIELGGPELLVETIQAYLGVPV
                     NHYAQVDFHGFGRLVDAIDGVEIWFPNPVRDQRSGLDVPEPGCVTLDARNALGFVRSR
                     AYQEYVDGRWRTDPTGDLGRIRRQQQFIIAALERAVDRGVRNPVTLDELIDSTLDAVT
                     IDDTLDGNDLLALGREFRRFEPSSLDVYQLPVVDDTVGGAAILRLVTQEADEVLDLFR
                     SSDPTRLSEDSVRVQVLNGTGEPGQASSASGALRDAGFALGATGDAARLGVERTEILY
                     PAGAEAGADLVRRWLVADAELVLDDSVDDITVVTGADWQGLRAEPLPAPEPTEGSSTT
                     LPTSTTTTTDDETTTTTALGTVSGAIPDDVVCG"
     gene            3178..4095
                     /locus_tag="VLR27_09165"
     CDS             3178..4095
                     /locus_tag="VLR27_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HSP03657.1"
                     /translation="MKGIVLAGGRATRLGPASRATSKQLMHVYDKPLVYYPISTLLHA
                     RINEILVISTPEQIPAFEALLGDGSQWGCSFSYAAQAEPRGLADAFIVGEDFIGGDDV
                     ALILGDNIFYGVGLGEQLQRFTTPRGAVVFGLWVPDPERYGVLDLADDGSVRAILEKP
                     AEPPSPYMVPGLYFYDGSVVEVAKGIEPSARGEIEITDVNNHYLAQGALEVCRLPFAT
                     DWFDVGTFDAMLDAQAWVAGLQRRKGLLIGSPEAAAYREGFIGADELVALAEAERHGG
                     DLAKSGYGESLLRVLSFEEADVTGEGPSW"
     gene            4095..4646
                     /gene="rfbC"
                     /locus_tag="VLR27_09170"
     CDS             4095..4646
                     /gene="rfbC"
                     /locus_tag="VLR27_09170"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HSP03658.1"
                     /translation="MVVDLSTEGTDIDGLVVVTMKQVTDERGTIREFYRESAFVDAGL
                     PSLGPWVQVNVTETGRGAIRGMHGEAMTKLVSVAAGRAHGAYVDARSGSDTFGAVVQV
                     ELVPGVQVLVPPGVCNGFQALEDGTQFLYCFDVEWQPGMAGVACTPLDPALGIDWPIA
                     VDPDDGSQVSAKDRSAPRFAELP"
     gene            4658..5617
                     /gene="rfbB"
                     /locus_tag="VLR27_09175"
     CDS             4658..5617
                     /gene="rfbB"
                     /locus_tag="VLR27_09175"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HSP03659.1"
                     /translation="MRLLVTGGAGFIGSNYVRWVLANTDDEIVVYDALTYAGNLSTLK
                     DVDDDPRYSFVHADICDPGPLEDAMRGCDAVVHFAAESHVDRSIEGSDDFVITNCFGT
                     NVVMDTARRLDIARVVHIGTDEVYGSVETGSSKEGDPLEPRSPYSASKAGSDLLALSY
                     HATHGLDVRVTRCTNNFGPYQYPEKAIPLFTTNLLEDKPIPLYGDGLNERDWIYVDDH
                     CAGVHLVLTDGAPGEIYNIGAGNETPNRVLVDKLLALTGKDESLVTYVEDRLGHDRRY
                     SVDITKITDLGWQRERTLDEALEATVAWYRDNEWWWRPLREGP"
     gene            5614..6471
                     /gene="rfbD"
                     /locus_tag="VLR27_09180"
     CDS             5614..6471
                     /gene="rfbD"
                     /locus_tag="VLR27_09180"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HSP03660.1"
                     /translation="MSRVLVTGAGGQLGVDLVRHCEDAGDDVVAADRATLDVGDRVAV
                     DAAVLDGGFDVVVNAAAWTAVDACEADPDRAYRDNALACRWLADACRRAGAHLVHVST
                     DYVFDGTKEGPYHEWDRPNPRSVYGASKWSGEHEIAVHAGGGATVVRTSWVFGEHGPN
                     MVRTVLALRDRDELAFVDDQRGCPTATADLAGVLRSLAVERVAGLFHVTNAGAVSWYE
                     FVRDVLEAAGEDPGKVRPISTADLDPPRPAPRPANSVLDNRAMRLSGLPPLRHYREAL
                     QQVVDALLG"
     gene            6494..7765
                     /locus_tag="VLR27_09185"
     CDS             6494..7765
                     /locus_tag="VLR27_09185"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HSP03661.1"
                     /translation="MSRIAIIGTGYVGLTTGACFSALGHDVVCADIDEAKVERLKQGD
                     IPILEAGLEEIVREGLESGRLTFVLGAATAAADCEFAYLCVPTPQGEDGSADLSYIEQ
                     AAREIAEVLPSESVVVNKSTVPVGSTRVVERALGRDDIAVVSNPEFLREGSAVHDFMN
                     PDRIVIGADDQSAAIKVASLYLGVAAPLIVTDPASAETIKYAANSFLATKISFINAVA
                     AICEAVGADINDVALGIGYDKRIGSEFLKPGPGWGGSCFPKDSRAMVKIAEEAGYDFH
                     LLRGVIQVNDEQYARTAEKAIELMGGDVDGKQVAAWGLTFKARTDDIRDSPSIEILGR
                     LAARGAKVRAYDPAVSHQLDGIEVVADPYDACDGADVLLVLTEWDEFKWLDLGEVKAR
                     MATPAVVDARNLLDRSALIRRGFTFRGIGRR"
     gene            7766..>8191
                     /locus_tag="VLR27_09190"
     CDS             7766..>8191
                     /locus_tag="VLR27_09190"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HSP03662.1"
                     /translation="MARVVVTGGAGFLGSHLCDRLLARGDEVVALDNLVTGNVANIEH
                     LFGHPGFTFVEHDVSKFIWVPGEVDAVLHFASPASPKDYLEMPIQTLKVGSLGTHNCL
                     GLAKEKGATFFLASTSEVYGDPQVHPQTEEYWGHVNPVGP"
BASE COUNT         1203 a         2960 c         2899 g         1129 t
ORIGIN      
        1 ggcctgcgtc gtgcacttcg acccgcccga ggacgacaag acctacctgc accgctccgg
       61 ccgcacggct cgcgccggtg cccagggcgt cgtcgtgtcc ttcgtgcggg acaacaaccg
      121 caaggacgtc aagaccctcc agcgcgacct cggtctcgcc caggtcgtga ccacgccgga
      181 ctcgaccctc ctcggcgacg gctcccgtca ccgcgaggcc acgtcccgcc agaagagcca
      241 gccggtggcg aagacccagc agtcgtcccc gcccaagggc ggcggcggca acggcggccg
      301 caaccgccag cggacgcgca cccgcgacgg tgaggaccgc gccgcgggca agagcgcacg
      361 ccgcgacgac cgtccggccc gcgacggcgg caagccccgc acccggacca agccggccga
      421 gcgccgcggc gccgacggcg tggtcgacgt cgacccccgc ccccgcaagg agcgccaggg
      481 caacggtggg cgcaccaacc cgccgtcgaa ggtgcaccgc aagggtggcg gcgccaagcg
      541 ccaggccatc gaggcgtccc gcccccgctg atcgctccgg cggtcgtctc tagccggagg
      601 cgaccgccaa cagcgtccgg gccttgagct cggtctcggc ccactcgccg gccgggtcgc
      661 tgtcgtgggt gatcccgccc ccggtgccga accggagcgt gggcccgcgc gtgccctccg
      721 gatcgatcca gaacgtgcgg atcgcgacgt tcagctcgcc gatcccacgg tcggcgtcca
      781 cccacccgac cccgccgcag tagacgccgc gggggacggg ctccagctgg cggatgacct
      841 ccatcgccgc cagcttcggc gcgcccgtga tcgaacccgg cgggaacgtg gcgtcgagca
      901 gctgcggcca accgacgccg tcgcacagct ccccggccac cgtcgagacc aggtgcacga
      961 ggccggggtg gtgctcgacc gcgagcaggg ccgggacgca gacggagccg aagtcgcaga
     1021 cccgcccgag gtcgttgcgg accaggtcca cgatcatcac gttctcggcc cggtccttgg
     1081 cgaggaacgc atcgggggtg gctgcggtgc ccttgatcgg tgaggaccac acgaggcggc
     1141 cctcgcgacg caggaagcgc tcgggtgacg ccgacgcgac gtggatgccg gcggccggga
     1201 cccgcaccac ggcggcgtag ggcgccgggt tgcccacggc cagggcggcc cccagcgcgg
     1261 cgacgtcggc gtcggggtcg accggcgccg ccagctggcg ggtgaggttg acctggtaca
     1321 cgtcgccggc ctcgatgcag gcccggatgg tgcgcacccc ctcctcgaag gcgtcccggt
     1381 cgagcgaggt ggtccacgcg tcccgatcgg ggccgaccca gcgcggaccc cgccacgggc
     1441 gggcgggacg gacggtggcg aagcgggcga acgtcggcgg accgtcgtag ggcacgacca
     1501 cggcccagaa gccctcgccg tccaggcacg cgggatcgtc ggtgacgtcg gtgaggtcgg
     1561 tgcacaaccg ctcgccgacc acggcgagcg gcggctggtc ggcgagccgg gccacgggcg
     1621 gaggctagag gggccgggtc gggcgctccc gggccggtcg gtagcctggg gccgatgtcc
     1681 ggcacggaga cacgtcgggc gaggcgcagc gtgctccaga agctggtgat catcacgaac
     1741 gtgttcgcga tggtcggctt cctgttcgcg gcgggttcgc tcgcctacgg ctacgagaag
     1801 ttcggccaga tcccccgcgt cgagatcggc gagacgctga gcgtcgagga cgccgacgac
     1861 ggccgtgagg tcatcgccga gaacttcctg atcgtcggcg tcgactccgc cgccgggctc
     1921 gatcccgacg accccgtggc cgccagccgg accgacatcg ccaacctgcg gtccgacacc
     1981 atgatgatcc tgcgggtcga cccgacctcg acccgagcag cgctgctgtc gctgccgcgc
     2041 gacctgtggg tgcccatccc cggccacggg aacggccgca tcaacgaggc gatcgagctc
     2101 ggcgggcccg agctgctcgt ggagacgatc caggcctacc tcggcgtccc ggtcaaccac
     2161 tacgcccagg tcgacttcca cgggttcggc cggctggtgg acgcgatcga cggcgtcgag
     2221 atctggttcc cgaacccggt ccgcgaccag cgcagcgggc tcgacgtgcc cgagccgggc
     2281 tgcgtcaccc tcgatgcgcg caacgccctc ggcttcgtgc gcagccgcgc ctaccaggag
     2341 tacgtcgacg gccggtggcg caccgatccc accggcgacc tcggccgcat ccgtcgccag
     2401 cagcagttca tcatcgccgc gctcgagcgg gccgtcgatc gcggcgtgcg caacccggtg
     2461 acgctggacg agctcatcga cagcaccctc gacgccgtca ccatcgacga caccctcgac
     2521 ggcaacgacc tgctcgccct cggtcgcgag ttccgtcgct tcgagccgtc ctcgctcgac
     2581 gtgtaccagc tgcccgtcgt cgacgacacc gtcggtggcg ccgccatcct gcgcctggtc
     2641 acccaggagg ccgacgaggt gctggacctg ttccgcagca gcgaccccac ccggctcagc
     2701 gaggactccg tgcgggtcca ggtgctcaac ggcaccgggg agccggggca ggcgtcgagt
     2761 gcctccggcg cgctgcgcga cgccgggttc gccctcggcg ccaccggcga tgcggcgcgg
     2821 ctcggcgtcg agcgcaccga gatcctgtac ccggcgggtg ccgaggccgg tgctgacctg
     2881 gtccgccgtt ggctggtggc ggatgccgag ctcgtgctcg acgactcggt cgacgacatc
     2941 acggtggtga ccggggcgga ctggcagggc ctgcgtgcgg agccgctgcc ggcaccggaa
     3001 ccgaccgagg gatcgtccac gaccctcccg acgtcgacca ccacgacgac ggacgacgag
     3061 accaccacca cgacggcgct cggcacggtc tcgggggcca tccccgacga cgtggtctgt
     3121 ggctgacgcc gccgccacgg acgacgacca gccgcgcgtc tgccaggatc ggcggccatg
     3181 aagggcatcg tcctcgctgg tgggcgggca acgcggctgg ggccggcgag cagagcgacc
     3241 tcgaagcagc tgatgcacgt ctacgacaag ccgctcgtct actacccgat ctccacgctg
     3301 ctgcacgcgc ggatcaacga gatcctcgtc atctcgacgc ccgagcagat cccggccttc
     3361 gaggcgctgc tcggggacgg ctcgcagtgg gggtgctcgt tctcctacgc ggcgcaggcc
     3421 gagccgcgcg ggctcgccga tgccttcatc gtcggcgagg acttcatcgg tggcgatgac
     3481 gtcgcgctga tcctgggcga caacatcttc tacggggtcg gcctgggcga gcagctgcag
     3541 cggttcacga cgccccgggg tgcggtcgtg ttcggcctgt gggtgccgga tccggagcgg
     3601 tacggcgtgc tcgacctggc cgacgatggg tcggtgcggg cgatcctgga gaagccggcg
     3661 gagccgccgt cgccgtacat ggtgccgggg ctgtacttct acgacgggtc ggtggtggag
     3721 gtggccaagg gcatcgagcc cagcgcccgg ggtgagatcg agatcaccga cgtgaacaac
     3781 cactacctcg cccagggtgc gctggaggtg tgccggctgc cgttcgccac ggactggttc
     3841 gacgtcggca ccttcgatgc catgctcgac gcgcaggcgt gggtggcggg cctgcagcgc
     3901 cgcaagggcc tgctgatcgg ctccccggag gcagcggcgt accgggaggg cttcatcggc
     3961 gccgacgagc tggtggcctt ggccgaggcc gagcgccacg gcggcgatct ggccaagagc
     4021 ggctacggcg agtcgctgct gcgggtcctg agcttcgagg aggccgacgt gaccggggag
     4081 ggcccgtcgt ggtgatggtc gtggacctgt cgaccgaggg caccgacatc gatggcctgg
     4141 tcgtggtgac gatgaagcag gtcaccgacg agcggggcac gatccgcgag ttctaccggg
     4201 agtccgcgtt cgtcgacgcc ggcctgccgt cgctggggcc gtgggtgcag gtgaacgtga
     4261 ccgagaccgg tcggggcgcc atccggggca tgcacggtga ggcgatgacc aagctggtgt
     4321 ccgtcgccgc cggccgggcg catggcgcct acgtggatgc ccggtcgggg tcggacacgt
     4381 tcggggcggt cgtgcaggtc gagctggtcc ccggtgtgca ggtgctggtg ccacccgggg
     4441 tgtgcaacgg cttccaggcc ctggaggacg gcacgcagtt cctgtactgc ttcgacgtcg
     4501 agtggcagcc gggcatggcc ggcgtggcgt gcacgccgct cgatccggcg ctgggcatcg
     4561 actggcccat cgccgtggac cccgacgacg ggtcgcaggt gtcggccaag gaccgcagcg
     4621 caccccgctt cgccgagctt ccgtagcctg ctgcgtcgtg cgactcctcg tcaccggcgg
     4681 tgccggcttc atcggttcga actacgtgcg ttgggtgctg gccaacaccg acgacgagat
     4741 cgtcgtctac gacgccctga cctatgccgg gaacctgtcg acgctcaagg atgtcgacga
     4801 cgatccccgc tactccttcg tgcacgccga catctgcgat cccggtcccc tcgaggacgc
     4861 gatgcggggc tgcgacgcgg tggtgcactt cgccgccgag agccacgtcg accgctccat
     4921 cgagggcagc gacgacttcg tcatcaccaa ctgcttcggc accaacgtgg tgatggacac
     4981 cgcccgccgc ctcgacatcg cacgggtcgt gcacatcggc accgacgagg tctacggatc
     5041 ggtcgagacc ggctcgtcga aggagggcga cccgctcgag ccccgctcgc cctacagcgc
     5101 gtccaaggcc ggctcggacc tgctcgcgct cagctaccac gccacccacg gcctcgacgt
     5161 ccgggtcacc cgctgcacca acaacttcgg gccctaccag tacccggaga aggcgatccc
     5221 gctgttcacc accaacctgt tggaggacaa gccgatcccg ctctacggcg acggcctcaa
     5281 cgagcgggac tggatctacg tcgacgacca ctgcgccggc gtccacctcg tcctcaccga
     5341 cggtgccccg ggcgagatct acaacatcgg cgccggcaac gagaccccca accgggtgct
     5401 ggtcgacaag ctcctcgccc tcactggcaa ggacgagtcc ctcgtcacct acgtcgagga
     5461 ccgcctcggc cacgaccgcc gctactccgt cgacatcacc aagatcaccg acctcggctg
     5521 gcagcgcgaa cggaccctcg acgaagccct cgaggccacc gtcgcctggt accgcgacaa
     5581 cgagtggtgg tggcgaccgc tgcgcgaggg cccatgagcc gggtcctggt caccggcgcc
     5641 ggcggccagc tgggtgtcga cctggtccgg cattgcgagg acgcgggcga cgacgtggtc
     5701 gctgccgacc gagccaccct cgacgtcggc gatcgggtcg cggtcgacgc cgcggtgctc
     5761 gacggtggct tcgacgtcgt cgtcaacgct gcggcatgga ccgccgtcga cgcctgcgag
     5821 gccgaccccg accgcgccta ccgggacaac gcgctggcgt gccgctggct cgccgacgcg
     5881 tgccggcggg ccggcgccca cctcgtccac gtctccaccg actacgtgtt cgacgggacc
     5941 aaggaggggc cgtaccacga gtgggaccgc ccgaaccccc ggtcggtgta cggggcgtcg
     6001 aagtggtcgg gggagcacga gatcgccgtc cacgccggtg ggggcgccac cgtcgtccgc
     6061 acgtcctggg tgttcggcga gcacgggccg aacatggtgc gcaccgtgct ggcgctgcgc
     6121 gaccgggacg agctggcctt cgtcgacgac cagcgggggt gcccgacggc gacggccgac
     6181 ctcgccggcg tgctgcgatc gctcgccgtc gagcgggtgg ccggcctgtt ccacgtcacc
     6241 aacgccggtg cggtgtcgtg gtacgagttc gtgcgcgacg tgctcgaggc ggcgggggag
     6301 gacccgggca aggtccgccc gatcagcacc gccgacctcg acccgccccg gccggcgccg
     6361 cgaccggcca actcggtgct cgacaaccgg gcgatgcgcc tctcgggcct cccgccgctg
     6421 cgccactacc gtgaggcgct gcagcaggtc gtcgacgccc tgctgggctg accccgtcgg
     6481 cggaggagcg gacatgagca ggatcgcgat catcggcacc ggctacgtcg gcctcaccac
     6541 cggggcgtgc ttcagcgcgc tgggccatga cgtggtgtgc gccgacatcg acgaggccaa
     6601 ggtcgagcgc ctcaagcagg gcgacatccc gatcctggag gccggcctcg aggagatcgt
     6661 gcgcgagggg ctcgagtcgg gtcggctcac gttcgtcctc ggtgcggcca cggctgcggc
     6721 cgactgcgag ttcgcctacc tgtgcgtgcc cacgccccag ggcgaggacg gctccgcgga
     6781 tctgtcctac atcgagcagg cagcgcggga gatcgccgag gtcctcccgt cggagtcggt
     6841 ggtggtgaac aagtcgacgg tgccggtcgg ttccacccgc gtggtggagc gggcgctcgg
     6901 gcgcgacgac atcgccgtgg tctcgaaccc cgagttcctg cgcgagggct cggccgtgca
     6961 cgacttcatg aacccggacc gcatcgtgat cggcgccgac gaccagtccg ccgccatcaa
     7021 ggtcgcctcc ctgtacctcg gcgtggccgc cccgctcatc gtcaccgacc cggcctcggc
     7081 cgagacgatc aagtacgccg ccaactcgtt cctggccacc aagatctcct tcatcaacgc
     7141 cgtcgccgcg atctgcgagg cggtgggcgc cgacatcaac gacgtcgcgc tcggcatcgg
     7201 ctacgacaag cgcatcggct cggagttcct caagccgggg ccgggatggg ggggaagttg
     7261 ttttccgaaa gattcgcgcg caatggtgaa gatcgcggag gaagctggtt acgacttcca
     7321 cctccttcgg ggcgtcatcc aggtcaacga cgagcaatac gcacgtaccg ccgagaaggc
     7381 catcgagctc atgggtggcg acgtcgacgg caagcaggtg gcggcgtggg gcctgacctt
     7441 caaggcgcgg acggatgaca tccgggactc gccgtccatc gagatcctcg gccggctggc
     7501 cgcccgcggc gccaaggtcc gggcctacga cccggcggtg tcgcaccagc tcgacggcat
     7561 cgaggtggtc gctgacccct acgacgcctg cgacggcgcg gacgtgctgc tggtgctgac
     7621 cgagtgggac gagttcaagt ggctcgacct cggcgaggtg aaggcccgca tggccacacc
     7681 ggcggtcgtc gacgcccgca acctcctcga ccgctcggcg ctcatccgcc gcgggttcac
     7741 cttccgcggc atcggacggc gctgagtggc ccgggtcgtc gtcacgggag gcgccggctt
     7801 cctcggctcc cacctgtgcg accgcctgct cgcgcggggt gacgaggtcg tcgccctcga
     7861 caacctcgtc accggcaacg tcgccaacat cgagcacctc ttcggccacc ccggcttcac
     7921 gttcgtcgag cacgacgtct cgaagttcat ctgggtgccg ggcgaggtcg acgccgtgct
     7981 gcacttcgcc tcgccggcgt cgccgaagga ctacctggag atgccgatcc agaccctgaa
     8041 ggtcggctcc ctcggcacgc acaactgcct gggcctggcc aaggagaagg gcgccacgtt
     8101 cttcctcgcc tccacctccg aggtctacgg cgacccgcag gtccaccccc agaccgagga
     8161 gtactggggg cacgtgaacc cggtcggtcc c
//
LOCUS       DATISU010000223         4605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_151454, whole genome shotgun sequence.
ACCESSION   DATISU010000223 DATISU010000000
VERSION     DATISU010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151454"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..973)
                     /locus_tag="VLR27_09195"
     CDS             complement(23..973)
                     /locus_tag="VLR27_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSP03663.1"
                     /translation="MGSGGYERGRVDLDGGCHAWLQPDGGWGWSNAGLVVGDGASLLV
                     DTLFDLKLTQEMLDGFGPLTGANPIGTLVNTHANGDHCYGNELVTGADIVATEACAEE
                     MPGTPPELLAAMVEAGENGELGETGAFFARIFGPFEFRGITLTPPTSTFTGRREVDVA
                     GTTVELLEVGPAHTAGDCIAHVPGASTVFTGDICFIEGTPIMWAGPIGNWIDACDLII
                     EDLHPDVVVPGHGPVTDVEGVRRMRDYLVFVDAQARARHADGMPLEEAIRDIALGEFA
                     GWIDRERLAVNVDSVYRELDPERGRTDVVTLFGMMAALAP"
     gene            complement(1003..1536)
                     /locus_tag="VLR27_09200"
     CDS             complement(1003..1536)
                     /locus_tag="VLR27_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03664.1"
                     /translation="MDLLRLELIGGPGRVETAVGSPAWERGVLGVDVSALAVEGVDHT
                     VVQVLFDDDAPAFDPDLLDAPLVAELRRPSGEVVVRDLFDHDRFRQALRADQESGGRE
                     LRGVLVLTSGELPPPYVRLAFLPLDVGGTAGTTLAVVRTDLPDLVMAVDDALEREEID
                     DDEHQALLTSIEQRHPA"
     gene            1602..2738
                     /locus_tag="VLR27_09205"
     CDS             1602..2738
                     /locus_tag="VLR27_09205"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSP03665.1"
                     /translation="MLLATREIARSKVRFGLLAGAVGLLVFLILFQQALFGGLVQGFI
                     GALENQDSPVLVYNEQARRNVEGSFLVPEQIEALGAVEGVEQAVAIGEATYTVEAGGE
                     LVDGVLFGYPLDAGGLGAPTTLTEGRLPEAPNEAVASSADAADGFDIGDTVRIAADGG
                     PAIEVVGLGEDLRFSVAPTLFVDYSTFEDAQRAVNPNAQAVLPSIAAVRPADGADPAE
                     LTARINGEVDGVEALTNADAIDSAPGVSAVNQSFQIILALAFGVVTLVVAFFFLILTV
                     QKAKPLTLLRAVGAPSGYLIRNLVVQILFVMGAGIAIGIGMVFAVRPISTGGAIELDL
                     EPSTVIPTIIGLTVLALVGSIGAIRRVLRIDPIRATVDTGSELA"
     gene            2735..3838
                     /locus_tag="VLR27_09210"
     CDS             2735..3838
                     /locus_tag="VLR27_09210"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP03666.1"
                     /translation="MRLAVRELLRRPGRFTVVGGALTVLVLLLLFLGGLLDGLYLNST
                     GAVRTLDADGIVFSDDARQSFLRSEITAEQRAEIDEVDGVAATGGLGFALLGVQLPDG
                     DEVVDGAVIGYELSSDELPEPPGTGEAHADRQLADEGASVGDTVLVGPAEVPLTIVGF
                     VDDTNYLQQGGLWVEPETWRSVLAENRPDAVVADGSFQSLVVRVDDGAEIDTVNSAID
                     DATGATETLAEDAAIDAIPGITEQNATFTAVIGVTIFVAGLVIALFFALLTLERLGLY
                     AVLKAVGGSSRTLVAGVVVQSVVVAAGAFVLGGLLTLGLLQILPDGIPLQLEPSRALS
                     TLASITVAAVVGALVSLRRIVRIDPASAIGAGA"
     gene            3852..4529
                     /locus_tag="VLR27_09215"
     CDS             3852..4529
                     /locus_tag="VLR27_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP03667.1"
                     /translation="MPSPALQLTDVRKTYRSGDEEVVALDHATLEVGRDELVALVGPS
                     GSGKTTLLSVAGALLGPTEGEVRVGDTDVSDLSEKERTAFRGKEIGFVFQAVNLVPFL
                     TARENLLVVAEMNGNLDKAAKQRADQLLDELGLSHRTGNQPGELSGGERQRVAIGRAL
                     MNEPSLVLFDEPTSALDTELGEQVMELIGREMRSRGVGGIFVTHDTRMTHHADRTLEI
                     IDGRLKA"
BASE COUNT          609 a         1747 c         1585 g          664 t
ORIGIN      
        1 cgaaacaggc cgatacccga cgtcaggggg cgagcgcggc catcatcccg aacagggtga
       61 cgacgtcggt gcggccccgc tcggggtcga gctcccggta gacgctgtcg acgttcacgg
      121 ccaggcgctc ccggtcgatc cagccggcga actcgccgag cgcgatgtcg cggatcgcct
      181 cctcgagcgg catgccgtcg gcgtggcggg cccgggcctg ggcgtcgacg aacaccaggt
      241 agtcccgcat gcgacgcacg ccctcgacgt cggtgaccgg gccgtggccg gggacgacga
      301 cgtcggggtg caggtcctcg atgatgaggt cgcacgcgtc gatccagttg ccgatggggc
      361 cggcccacat gatcggcgtg ccctcgatga agcagatgtc cccggtgaac acggtggacg
      421 cacccggcac gtgggcgatg cagtcgccgg cggtgtgggc gggcccgacc tcgagcagct
      481 cgacggtggt gccggccacg tcgacctccc gccgtccggt gaaggtggag gtgggcgggg
      541 tgagggtgat gccgcggaac tcgaaggggc cgaagatgcg ggcgaagaac gcgccggtct
      601 cgcccagctc accgttctcg ccggcttcga ccatggcggc gagcagctcg ggcggcgtgc
      661 ccggcatctc ctcggcgcag gcctcggtgg ccacgatgtc ggcgccggtc accagctcgt
      721 tgccgtagca gtggtcgccg ttggcgtggg tgttcaccag cgtgccgatg gggttggcgc
      781 cggtgagcgg cccgaacccg tcgagcatct cctgggtcag cttcaggtcg aacagcgtgt
      841 cgaccagcag gctggccccg tcgccgacca ccaacccggc gttggaccac ccccagccac
      901 cgtccggttg cagccacgcg tggcagcccc cgtccaggtc gacgcggccc cgctcgtatc
      961 ccccgcttcc catccggcgg aacgtatctc aggccgggtg tctcaagccg ggtggcgctg
     1021 ctcgatggac gtcagcaggg cctggtgctc gtcgtcgtcg atctcctcgc gctcgagcgc
     1081 gtcgtccacc gccatcacca gatcgggcag gtcggtgcgc acgacggcga gcgtggtgcc
     1141 ggcggtcccg cccacgtcga ggggcaggaa cgccagccgc acgtacgggg gaggcagctc
     1201 gccgctggtg aggacgagca cgccgcgcag ctcccgtccg ccggactcct ggtcggcccg
     1261 gagggcctgg cggaaccggt cgtggtcgaa caggtcgcgc accacgacct cgcccgacgg
     1321 ccggcgcagc tcggcgacca gcggcgcgtc gagcaggtcg gggtcgaacg ccggcgcgtc
     1381 gtcgtcgaag agcacctgca ccacggtgtg gtcgacgccc tcgacggcga gggcgctgac
     1441 gtcgacgccg agcacgccgc gctcccaggc gggcgacccg acggcggtct cgacccgtcc
     1501 gggtccgccg atgagctcca gtcgcaagag gtccacggct cgatcctggc agccgtggcg
     1561 cccgcggtgg cagggggcag cgctgtccct aacctgcgcc cgtgctcctg gccacccgcg
     1621 agatcgcccg gtccaaggtc cgcttcggcc tgctcgccgg cgccgtcggc ctgctcgtgt
     1681 tcctgatcct gttccagcag gcgctgttcg gcggcctcgt ccagggcttc atcggtgcgc
     1741 tcgagaacca ggactcgccg gtcctcgtct acaacgagca ggcccggcgc aacgtggagg
     1801 gcagcttcct ggtgcccgag cagatcgagg cgctcggtgc cgtcgagggc gtcgagcagg
     1861 cggtggcgat cggcgaggcg acctacacgg tggaggcggg tggcgagctg gtcgacggcg
     1921 tgctgttcgg ctacccgctc gacgccggcg gcctcggcgc ccccaccacg ctcaccgagg
     1981 gccgcctccc cgaggcgccc aacgaggccg tcgccagcag cgccgatgcg gccgacgggt
     2041 tcgacatcgg cgacaccgtg cgcatcgctg ccgatggtgg cccggcgatc gaggtcgtcg
     2101 gcctcgggga ggacctgcgc ttcagcgtgg cccccaccct gttcgtcgac tactcgacct
     2161 tcgaggacgc gcagcgggcg gtgaacccca acgcccaggc ggtgctgccc tccatcgctg
     2221 ccgtccggcc cgccgacggc gccgacccgg ccgagctcac cgcccgcatc aacggcgagg
     2281 tcgacggcgt cgaggcgctc acgaacgccg acgccatcga ctcggcgccc ggcgtgtcgg
     2341 cggtgaacca gtcgttccag atcatcctcg ccctggcctt cggcgtcgtc accctggtcg
     2401 tggcgttctt cttcctgatc ctcaccgtgc agaaggccaa gccgctcacc ttgctgcggg
     2461 cggtgggcgc gccgtcgggc tacctcatcc gcaacctggt cgtgcagatc ctgttcgtga
     2521 tgggcgccgg catcgccatc ggcatcggca tggtcttcgc cgtccgcccg atctccaccg
     2581 gaggcgccat cgagctcgac ctcgagccct ccaccgtcat ccccaccatc atcggcctca
     2641 ccgtgctcgc cctcgtcggc agcatcgggg ccatccgccg ggtgctgcgc atcgacccga
     2701 tccgggccac cgtcgacacc gggagcgagc tggcatgagg ttggccgtcc gcgagctgct
     2761 ccgccgtccc ggccgcttca ccgtcgtcgg tggcgcgctc accgtcctgg tgctgctgct
     2821 cctcttcctc ggcggcctcc tcgacggcct ctacctcaac tccacgggcg cggtccgcac
     2881 gctcgacgcc gacggcatcg tcttctccga cgacgcccgc cagtcgttcc tgcgctccga
     2941 gatcaccgcc gagcagcggg ccgagatcga cgaggtcgac ggcgtggcgg cgaccggcgg
     3001 cctcggcttc gcgctcctcg gcgtgcagct ccccgacggc gacgaggtcg tcgacggagc
     3061 ggtcatcggc tacgagctgt cctccgacga gctgcccgaa ccgccgggca ccggcgaggc
     3121 ccacgccgac cgccagctcg ccgacgaggg tgcgtcggtg ggcgacacgg tcctcgtcgg
     3181 gccggccgag gtgccgctga ccatcgtcgg cttcgtcgac gacaccaact acctccagca
     3241 gggcggcctc tgggtcgagc ccgagacctg gcgctcggtg ctggccgaga accggcccga
     3301 cgccgtggtg gccgacgggt cgttccagtc cctggtcgtc cgggtcgacg acggtgcgga
     3361 gatcgacacc gtcaacagcg ccatcgacga cgcgaccggc gccaccgaga ccctggccga
     3421 ggacgccgcc atcgatgcca tccccggcat cacggagcag aacgccacct tcaccgcggt
     3481 catcggcgtg acgatcttcg tcgccggcct cgtcatcgcc ctgttcttcg ccctcctcac
     3541 cctcgagcgc ctcgggctgt acgccgtgct caaggcggtc ggtgggtcgt cgcgcacgct
     3601 ggtcgccggc gtggtcgtcc agtcggtcgt ggtcgccgcc ggtgcgttcg tgctcggcgg
     3661 cctgctcacc ctcgggctcc tccagatcct ccccgacggg atcccgctcc agctcgagcc
     3721 gtcccgcgcc ctgtccaccc tcgccagcat caccgtggcc gccgtcgtcg gcgccctggt
     3781 gtcgctccgg cgcatcgtcc gcatcgaccc ggcgtccgcc atcggcgccg gcgcctgacc
     3841 cgggaagccc catgcccagc cccgccctcc agctcaccga cgtccgcaag acctaccgct
     3901 ccggcgacga ggaggtggtc gccctcgacc acgccaccct cgaggtcggc cgggacgagc
     3961 tcgtcgccct cgtcggcccg tccgggtcgg gcaagaccac gctgctgtcg gtggccggcg
     4021 cgctgctcgg gccgaccgag ggcgaggtcc gcgtcggcga caccgacgtc agcgacctgt
     4081 cggagaagga gcggaccgcg ttccggggca aggagatcgg cttcgtgttc caggccgtga
     4141 acctggtgcc gttcctcacc gcccgcgaga acctgctcgt cgtcgccgag atgaacggca
     4201 acctcgacaa ggcggccaag cagcgcgccg accagctgct cgacgagctc ggcctctccc
     4261 accgcaccgg caaccagccc ggcgagctgt ccggcggcga gcgccagcgg gtggccatcg
     4321 gccgggcgct catgaacgag cccagcctcg tgctcttcga cgagccgacc tcggccctcg
     4381 acaccgagct cggcgagcag gtcatggagc tcatcggccg cgagatgcga tcccgggggg
     4441 tcggcggcat cttcgtcacc cacgacaccc gcatgaccca ccacgccgac cgcacgctcg
     4501 agatcatcga cggtcgcctg aaggcatgac cgccgccagc gtccacgacg tcctgcccct
     4561 ggagtccgac ctgcccggca ccggctggtt cgccatcgac gaggg
//
LOCUS       DATISU010000224         9018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_617203, whole genome shotgun sequence.
ACCESSION   DATISU010000224 DATISU010000000
VERSION     DATISU010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617203"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VLR27_09220"
     CDS             <1..771
                     /locus_tag="VLR27_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSP03668.1"
                     /translation="RVAGFDVVRQAGDVRRAIGVALQDAAIDPLMTGSELVHLQAVLH
                     GIPTKVADQRGKDLLERVGLTAAADRRVGTYSGGMRRRLDLALSLVHEPEVLFLDEPT
                     TGLDPMSRLALWEEVRYLNRERGTTVLLTTQYLEEADQLARRIAIIDHGRIVKKGDPR
                     ALKDEVGSPTLMIEVEGDPAPALARLVRFGEQRPCEPGMIAIGLEGGAQQLTAVVREL
                     DEAGVAVRHMELNAPTLDDVFAEATGRRLEGADEPAPT"
     gene            768..1589
                     /locus_tag="VLR27_09225"
     CDS             768..1589
                     /locus_tag="VLR27_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP03669.1"
                     /translation="MTATVPADVVVNRPSPVRQSLLLSRRAVFAVLRQPQVLVPSIFF
                     PLFFAALNAAAFDRITSEQPVFAAQFDSFLDFLLPATVLQGVLFGSTQAGTEMANDIE
                     NGFFDRLLASPVSRTSILVGRLAGGAVLGAVQALFFTAVLVVFGARVDGGFGAVAVLV
                     VCAALLGMGIGGFGVMLGIRTGSAEAVQGAFPLIFVLMFTSSAFFPRELMTGWYRTVA
                     TYNPISWILEALRDLISIGWSFQDAAVAIAVSAAVAAFAVVLSLRALRRRLVVAA"
     gene            1586..2413
                     /locus_tag="VLR27_09230"
     CDS             1586..2413
                     /locus_tag="VLR27_09230"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP03670.1"
                     /translation="MTAVDARVDLGTTRHGSGAIIREMVARNLRGIRRQPATFIPTLI
                     MPIFFVITFSGAYGFAGRAFGNGNAMSWYAPMAIIQGASVAGMTMGLSTARDFESGFF
                     DRFLLAPMSRATLLGGALAAALVRALLTTTVVLLAAVIGGVAIPGGALGIATLYLSAL
                     GIAIVAALWALGLVYRVQSTNVGPLIQVGIFMAIFLSTAQVPISAMSGWLPGVARINP
                     MTNVLRLARAGFVPDELNPPSGFGVGWAEMWPGLLALGATIAVLTVFVLRSMRRLTP"
     gene            complement(2445..3644)
                     /locus_tag="VLR27_09235"
     CDS             complement(2445..3644)
                     /locus_tag="VLR27_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSP03671.1"
                     /translation="MAGLDEEVEVLVVGGGPAGTVLATLLASRGRSVAVFERGTPESL
                     ALGTGILLQPNGLAVLYGLGLRDELHRVSVQHRAVPIEDERGRLITTGEMPDFGHGLD
                     HALALLRPTLQRILWSSLEARGGLVRFGCDVRAVDTAAGEVDVFAGGQTYRISAEVIV
                     GADGIGSVVRRSGAFGARPVAGAHHVIRTVVDGEFNVSGSEYWTGLGLFGASPAGEGK
                     TYAYASANDPEVLDAVRTRDLARVVDLWSGALPVAAPLLHQIRSVDDILLNEIGRVRC
                     RRFADRRAVLIGDAAHAMAPNLGQGANSALVDAAVLARALTEAPTKLDALGRYDRQRR
                     RPVRRVQRTSDLLAEVSHLRHTGLRRARDRVFRIAGHPTLVRRQIRTTQQVDPAQLRR
                     DVEALAG"
     gene            complement(3683..3958)
                     /locus_tag="VLR27_09240"
     CDS             complement(3683..3958)
                     /locus_tag="VLR27_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03672.1"
                     /translation="MGDPAPPVTLVGDDASGYIVSGIVTTVLIVFVVSAVVGIVARFF
                     GRRGSWVVAVPLGVLGFIAGPVGILTVASLTGGFAFGNVAAARRRGW"
     gene            complement(3994..4944)
                     /locus_tag="VLR27_09245"
     CDS             complement(3994..4944)
                     /locus_tag="VLR27_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HSP03673.1"
                     /translation="MSWTADDIPDQTGRTAVVTGANGGLGLETAKALAAKGAHVVMAA
                     RDQAKAAGAEQEIRSHAPDASLEIVSLDLGDQASVKDAAATITEAHPVVDLLVNNAGV
                     MAMPERKTADGYEMQLGVNHLGHWTFTAGLLAPLLRSAADGRRPRVVTVTSVARFTGG
                     PIDPSNPHLAGRYDPWRAYGQAKLANYFFGLGLQQQAERAGVDLASLLAHPGLTNTDL
                     QSRTHREGGAGVLGAMSDRITDVLGMSSEAGTRMQLRAATDPKAKGGQLYGPAFASFG
                     PAVTRPILRRDLQARIDQLWAVSERETGVPLDIAEAARSL"
     gene            complement(4953..6548)
                     /locus_tag="VLR27_09250"
     CDS             complement(4953..6548)
                     /locus_tag="VLR27_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03674.1"
                     /translation="MRRQDRPLVAVLGLVSICSTLLAVSAATAAPLAEPALLPTPQVE
                     CDPDDAAETDIQGRVTPDDHASGRAERGFWCNAERIGTFGGEAVDGTVGGFKVERYVD
                     ASGRECAYYDSTLLLGTDLFDGGIGTNVLDMSDPANPVKTASLVTPAMLSPHESLLVN
                     QERGLLVATLGNPAFNVGILDVYDLTEDCRTPKLLSSSPAGILGHESGFAPDGMTFYS
                     ASPATQTLVPVDLSDPTLPVPLTSYRINSHGLSLSADGTRAYVASLDGLVILDVSEVQ
                     AREPDPVIREVSRLSWESMSIPQNAIPITIDGHPYVVEIDEFGAQEAVGAARIIDIAD
                     ETRPQVLSNLRLEVHQPEHFEQQSADPGSSSPIQGYAAHYCNVPTRVDPTIVACSMIL
                     SGLRVFSIVDPANPVEIAYYNAPSDPNAPTAGSYAMASPSFVPERDEIWYSDGHSGFH
                     VVRLADGVWPAAGSAPVQPAPPTTEPTTTAAPSTAAAPAQAAAPTLPATGGGAPLGLA
                     LAAALSGAAALWLATRGRRAARP"
     gene            6632..>9018
                     /locus_tag="VLR27_09255"
     CDS             6632..>9018
                     /locus_tag="VLR27_09255"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSP03675.1"
                     /translation="MPGAPVLDQFSPAVRAWFETSFPEPTAPQVAGWPTIASGAHTLI
                     LAPTGSGKTLSAFLWAIDRLTSTPPPEERTHRTRVVYLSPLRALAVDVEKNLRAPVQG
                     IRLAAERLGLPYHEPTVGMRTGDTPADERRKLIRNPPDILITTPESLYLMLTSQARET
                     LQGVEAVIVDEIHAVAKTKRGSHLSVTMERLVEVTDRADGPQRIGLSATQRPLEEIAE
                     FLGGRDDTGSWRRVDIVDAGVRKELDIEVIVPVEDMGDLGKVIEEPISGPASLGPVRR
                     SIWPSMHPRLLELVQENRSTLIFVNARRLAERLATRLNELAHDEEDTGDAQLGVGHVD
                     DGSDVPELVKAHHGSISRERRLQIEDEHKSGKLKGLVATSSLELGIDMGAVDLVVQVE
                     SPGSVSSGLQRIGRAGHQVGEPSRGKLFPKHRADLVEAAVVVKRMQDGLIERTRYPRN
                     PLDVLCQQIVAMCALDEWQVDDLARILRRSASFSTLGDEVFHACLDLLAGRYPSEEFS
                     ELRPRIVWDRTAPGGGGGVVRGRAGAQRLAVTNPGTIPDRGLFGVFLPDGTRVGELDE
                     EMVYESRAGETFLLGASTWRIEEITFERVIVTPAPGLPGKMPFWHGEGPGRPLELGQG
                     IGQFVRELRDRGTTPDPVKTLREDHGLDELAALNLVSYLDEQAEATGVVPDDRTIVVE
                     RFRDEIGDWRVCVLSPFGAQVHAPWAMALQQTLGERWGVDVEMIWSDDGIVLRLPEAV
                     EELPVEELAIPPDEIRDIVLGQLPNTAMFASRFRECAGRALLLPRRRPDRRQPLW"
BASE COUNT         1199 a         3236 c         3277 g         1306 t
ORIGIN      
        1 cgcgtcgccg gcttcgacgt cgtccgccag gccggcgacg tgcgccgcgc catcggcgtc
       61 gcgctgcagg acgcagccat cgacccgctg atgaccggct cggagctggt gcacctgcag
      121 gcagtgctgc acggcatccc gaccaaggtc gccgaccagc gcggcaagga cctgctcgag
      181 cgggtgggcc tcaccgcggc cgccgaccgt cgggtcggga cctactcggg cggcatgcga
      241 cgccggctcg acctggcgct ctcgctcgtg cacgagcccg aggtgctctt cctcgacgag
      301 ccgaccaccg gcctcgaccc gatgagccgc ctcgcgctgt gggaggaggt ccggtacctg
      361 aaccgggagc ggggcaccac cgtgctgctc accacgcagt acctggagga ggccgaccag
      421 ctcgcccgcc gcatcgccat catcgaccac ggccgcatcg tgaagaaggg cgacccccgg
      481 gccctgaagg acgaggtcgg ctcaccgacg ctgatgatcg aggtggaggg cgacccggcc
      541 ccggcactcg cccgcctggt ccgcttcgga gagcagcgcc cgtgcgagcc cggcatgatc
      601 gccatcggcc tggagggcgg tgcccagcag ctgaccgccg tcgtgcgcga gctcgacgag
      661 gccggcgtgg ccgtgcggca catggagctg aacgccccga ccctcgacga cgtgttcgcc
      721 gaggcgaccg ggcgccgcct cgagggcgcg gacgagcccg ccccgacgtg accgccaccg
      781 tccccgccga cgtcgtggtg aaccggccca gcccggtccg ccagtcactg ctcctgtcgc
      841 ggcgggcggt gttcgccgtg ctgcgccagc cccaagtgct ggtgccgtcg atcttcttcc
      901 cgctcttctt cgccgcgctc aacgccgcgg ccttcgaccg catcacgtcc gagcagccgg
      961 tgttcgccgc gcagttcgac tcgttcctcg acttcctgct gccggccacc gtgctgcagg
     1021 gcgtcctgtt cggatccacc caggccggca ccgagatggc caacgacatc gagaacgggt
     1081 tcttcgaccg gctcctcgcc tcgccggtgt cacgcacctc catcctcgtc ggccgcctgg
     1141 ccggcggtgc ggtgctcggt gcggtgcagg cgctgttctt caccgcggtg ctggtcgtct
     1201 tcggcgcccg ggtcgacggc gggttcggtg ccgtcgccgt gctcgtggtg tgcgctgcgc
     1261 tgctcggcat gggcatcggc ggcttcggcg tgatgctcgg catccgcacc ggttcggccg
     1321 aggccgtgca gggcgcgttc ccgctcatct tcgtgctgat gttcacctcc tccgcgttct
     1381 tcccgcggga gctgatgacc ggctggtacc gcacggtcgc cacctacaac ccgatctcat
     1441 ggatcctcga ggcgctgcgc gacctgatca gcatcgggtg gtcgttccag gacgcggccg
     1501 tggccatcgc ggtgtcggcc gcggtcgccg ccttcgccgt cgtgctctcg ttgcgggcgc
     1561 tgcgccggcg gctggtggtg gcagcgtgac ggccgtcgac gcccgggtcg acctcggcac
     1621 gacccggcac ggctccggcg ccatcatccg cgagatggtg gctcgcaacc tgcggggcat
     1681 ccgtcgccag ccggccacgt tcatccccac gctgatcatg ccgatcttct tcgtgatcac
     1741 gttctccggc gcatacggct tcgccggccg ggccttcggc aacggcaacg ccatgtcgtg
     1801 gtacgccccg atggccatca tccagggcgc ctcggtcgcc ggcatgacga tggggctgtc
     1861 caccgcacgt gacttcgagt ccgggttctt cgaccgcttc ctgctcgccc cgatgagccg
     1921 ggccaccctc ctcggcggcg cgctcgccgc cgcactcgtg cgcgccctgc tgaccaccac
     1981 cgtcgtgctg ctcgccgcgg tcatcggcgg tgtggcgatc ccgggtggcg cgctcggcat
     2041 cgccaccctc tacctgtcgg cgctcggcat cgccatcgtc gccgccctct gggccctggg
     2101 tctcgtctac cgggtgcaga gcaccaacgt cggcccgctc atccaggtcg gcatcttcat
     2161 ggccatcttc ctgtcgaccg cgcaggtgcc gatctcggcc atgagcggct ggctcccggg
     2221 cgtcgcccgc atcaacccga tgaccaacgt gctccgcctg gcccgggccg ggttcgtgcc
     2281 cgacgagctg aacccgccgt cgggcttcgg cgtcggctgg gccgagatgt ggcccggcct
     2341 gctcgccctc ggtgccacca tcgccgtgct caccgtgttc gtgctccgga gcatgcgccg
     2401 cctgacgccc tgaggcgctg cctctgggtg gtacatcgac gcggtcagcc cgccagcgcc
     2461 tccacgtcgc gccgcagctg ggcggggtcg acctgctggg tggttcggat ctggcggcgg
     2521 accagcgtcg ggtgaccggc gatccggaag acccggtcgc gcgcccggcg caacccggtg
     2581 tgtcggaggt gcgatacctc ggcgagcagg tccgacgtgc gctggacccg gcggaccggt
     2641 cggcgtcgct gccggtcgta ccgtccgagc gcgtcgagct tcgtcggagc ctcggtgagc
     2701 gctcgagcga ggaccgccgc atcgacgagg gcgctgttgg cgccctgccc caggttcggg
     2761 gccatggcgt gcgcagcgtc gccgatgagc acggctcgtc gatcggcgaa ccggcggcag
     2821 cgcacgcggc cgatctcgtt caggaggatg tcgtcgacgg atcggatctg gtggaggagc
     2881 ggcgcggcga cgggaagtgc tcccgaccag agatcgacca cccgtgccag atcgcgcgtc
     2941 cgcacggcat ccagcacctc cgggtcgttc gcgctcgcat aggcgtaggt cttgccctcc
     3001 ccggccggcg aggcgccgaa cagcccgagg ccggtccagt actccgagcc ggacacgttg
     3061 aactccccat cgaccacggt ccggatcacg tggtgggcgc ccgcgaccgg acgcgcaccg
     3121 aaggctcccg accgacgcac cacggagccg atgccgtcgg cgccgacgat gacctcagcg
     3181 ctgattcggt aggtctggcc gccggcgaag acgtccacct cgccggcggc ggtgtcgacc
     3241 gcccgcacgt cgcagccgaa gcgcacgaga cccccgcggg cctcgagcga agaccacagg
     3301 atccgctgca gcgtgggtcg caggagcgcg agggcatggt cgagcccgtg cccgaagtcg
     3361 ggcatctcgc cggtggtgat cagccggccg cgctcgtcct cgatcggcac ggcccggtgc
     3421 tggacggaga cgcggtgcag ctcgtcgcgg agcccgagcc cgtacagcac tgccaggccg
     3481 ttgggctgca gcaggatccc ggtgccgagc gccaacgact cgggcgtccc gcgctcgaac
     3541 accgccacgg agcggccacg agatgcgagc aacgtcgcca acaccgtccc cgccgggcca
     3601 ccgcccacga ccaggacctc gacctcttca tccagcccgg ccacggcgag acgctcgacc
     3661 ttcgtgtccg gagctctcgc agctaccagc cgcgtcgtcg cgcggcggcg acgttcccga
     3721 aggcgaagcc gccggtcagc gacgcgacgg tgaggatgcc gacaggcccg gcgatgaacc
     3781 ccaggacgcc gagcgggacc gccacgaccc aggagcccct gcggccgaag aaccgggcga
     3841 cgatgcccac cacggcgctc accacgaaga cgatcaggac cgtcgtgacg atcccgctga
     3901 cgatgtagcc gctggcatcg tccccgacca gggtgacggg aggtgcagga tcgcccatac
     3961 gtcccgattg tgccgcaccg aaccgggcgc gcgtcagagg gagcgggcgg cctcggcgat
     4021 gtccagggga acgccggtct cccgctcgga gaccgcccag agctggtcga tgcgggcctg
     4081 caggtctctg cgcaggatcg gtcgggtgac ggcgggaccg aagctggcga aggcgggccc
     4141 gtagagctgg ccgccctttg ccttcgggtc ggtcgccgcc cgcagctgca tgcgggtccc
     4201 ggcctcggac gacatgccga ggacgtcggt gatccgatcg gacatcgcgc cgagcacgcc
     4261 ggccccgccc tcgcggtggg tgcggctctg caggtcggtg ttcgtgaggc cggggtgggc
     4321 gaggaggctg gccaggtcca cgccggcccg ctcggcctgc tgctgcagcc cgaggccgaa
     4381 gaagtagttg gccagcttgg cctggccgta ggcccgccac gggtcgtagc ggccggcgag
     4441 gtgcgggttc gaggggtcga tcgggccccc ggtgaagcgg gccacgctcg tgacggtgac
     4501 gacccgaggc cggcgaccgt cggccgccga acggagcagg ggcgcgagca ggccggcggt
     4561 gaaggtccag tggccgaggt ggttcacgcc gagctgcatc tcgtagccgt cggccgtctt
     4621 gcgctccggc atcgccatca cgccggcgtt gttcaccagg aggtcgacca ccgggtgcgc
     4681 ctcggtgatg gtcgcggctg cgtccttcac cgacgcttgg tcgccgaggt cgagggacac
     4741 gatctcgagc gaggcatcgg gtgcgtggct gcggatctct tgctcggcgc ccgccgcctt
     4801 ggcctggtcg cgagccgcca tcaccacgtg cgcacccttg gcggcgaggg ccttggccgt
     4861 ctcgagcccg agcccaccgt tggcgccggt gaccacggcg gtgcggccgg tctggtcggg
     4921 gatgtcgtcg gcggtccagg acatcgtgct cctcagggtc gtgcggcgcg gcggccccgc
     4981 gtggcgagcc acagcgccgc ggcgccggag agcgcagcgg cgagggccag acccaagggt
     5041 gccccaccgc cggtggccgg caacgtcggg gcggcggctt gcgccggagc ggccgcggtc
     5101 gatggtgccg cggtggtcgt cggctccgtc gtcggtgggg ccggttgcac cggggccgaa
     5161 cccgcggccg gccacacgcc gtcggccagg cgcaccacgt ggaagccgga gtggccgtcg
     5221 gagtaccaga tctcgtcgcg ctcggggacg aaggacgggc tggccatggc gtagctgccg
     5281 gccgtcggcg cgttcgggtc cgacggggcg ttgtagtagg cgatctcgac cgggttggcc
     5341 gggtccacga tgctgaagac gcgcaggccc gacaggatca tcgagcaggc gacgattgtc
     5401 gggtccacgc gggtcgggac gttgcagtag tgggcggcgt agccctggat cgggctcgag
     5461 ctccccgggt cggccgactg ctgctcgaag tgctcgggct ggtggacctc gaggcgcagg
     5521 ttggagagca cctgcggacg ggtctcgtcg gcgatgtcga tgatgcgggc ggcgccgacc
     5581 gcctcctgcg caccgaactc gtcgatctcg acgacgtagg ggtggccgtc gatcgtgatc
     5641 gggatggcgt tctgcgggat gctcatcgac tcccacgaca ggcggctgac ctcccggatc
     5701 accgggtcgg gctcgcgggc ctggacctcg gagacgtcca ggatgaccaa cccgtcgagg
     5761 gaggccacgt aggcgcgagt gccgtcggcg ctgagcgaga ggccgtggct gttgatgcgg
     5821 tagctggtga gcggcaccgg cagggtcggg tcggacaggt cgacgggcac gagcgtctgg
     5881 gtggccggcg acgccgagta gaaggtcatg ccgtcggggg cgaagccgct ctcgtggccg
     5941 aggatcccgg ccggtgacga cgacagcagc ttcggcgtcc ggcagtcctc ggtgaggtcg
     6001 tacacgtcga ggatgccgac gttgaacgcc gggttgccga gggtcgccac gagcaggccg
     6061 cgctcctggt tgacgagcag ggactcgtgg ggcgacagca tcgccggcgt cacgagcgac
     6121 gcggtcttca ccgggttcgc cgggtcggac atgtcgagca cgttcgtgcc gatgccgccg
     6181 tcgaacaggt cggtgccgag cagcagggtg gagtcgtagt aggcgcactc ccgaccgctg
     6241 gcgtcgacgt agcgctcgac cttgaaccca ccgacggtcc cgtcgacggc ctccccgccg
     6301 aaggtgccga tgcgctcggc gttgcaccag aagccgcgct cggctcgccc ggaggcgtgg
     6361 tcgtctggtg tcacccggcc ctggatgtcg gtctcggccg cgtcgtcggg gtcgcactcg
     6421 acctgcgggg tcgggagcag cgccggctcg gcgagcgggg cggcggtggc ggccgagacg
     6481 gcgagcaagg tcgagcagat cgagaccagg ccgagcacgg cgacgagcgg tcggtcctgg
     6541 cgtcgcaccc atgcgacgtt acgcggcgta cagggggaat gtccgattcg gcgcagtccg
     6601 gccgtcacgc cacgccgata ccgttcctcc gatgcctggg gcgcccgtgc tcgaccagtt
     6661 ctccccggcg gtgcgggcgt ggttcgagac gtcgttcccc gagcccacgg cgccgcaggt
     6721 cgcaggctgg cccaccatcg cctcgggtgc gcacacgctg atcctggcac cgaccgggtc
     6781 gggcaagacg ctgtccgcgt tcctgtgggc catcgaccgg ctcacgtcga cgcccccgcc
     6841 ggaggagcgc acgcaccgga cccgcgtcgt ctacctgtcg ccgctgcggg cactggccgt
     6901 ggacgtcgag aagaacctgc gggcgccggt ccagggcatc cgcctcgctg ccgagcgcct
     6961 cgggctgccg taccacgagc ccaccgtggg catgcgcacc ggcgacacgc cggccgacga
     7021 gcgccgcaag ctcatccgca acccgcccga catcctgatc acgacccccg agtcgctcta
     7081 cctgatgctc acctcccagg cccgggagac cctgcagggc gtggaggcgg tcatcgtcga
     7141 cgagatccac gccgtggcca agaccaagcg gggctcgcac ctgtcggtga cgatggagcg
     7201 tctggtggag gtcaccgacc gcgccgacgg gccgcagcgc atcgggctgt cggccacgca
     7261 gcgacccctg gaggagatcg ccgagttcct cggcggacgt gacgacaccg gctcgtggcg
     7321 ccgggtcgac atcgtcgatg ccggtgtccg caaggagctc gacatcgagg tcatcgtccc
     7381 ggtcgaggac atgggcgacc tcggcaaggt catcgaggag ccgatcagcg gccccgcgtc
     7441 gctcggtccg gtgcgccgca gcatctggcc gtcgatgcac ccgcgcctgc tggagctggt
     7501 gcaggagaac cgctccacgc tcatcttcgt gaacgcccgc cggctggccg agcggttggc
     7561 cacccggctg aacgagctgg cccacgacga ggaggacacc ggcgacgccc agctcggcgt
     7621 gggtcacgtc gacgatggca gcgacgtccc cgagctggtg aaggcccacc acgggtcgat
     7681 ctcccgggag cgccggctgc agatcgagga cgagcacaag tcggggaagc tgaaggggct
     7741 cgtggccacg tcgtcgctcg agctcggcat cgacatgggc gcggtcgacc tcgtggtgca
     7801 ggtcgagtcg ccgggaagcg tgtcctcggg cctccagcgc atcggtcgcg ccggccacca
     7861 ggtgggcgag ccgagccggg gaaagctgtt cccgaagcac cgggccgacc tggtcgaggc
     7921 ggccgtcgtc gtgaagcgga tgcaggacgg gctcatcgag cgcacccgct atccgcgcaa
     7981 ccccctcgac gtgctgtgcc agcagatcgt ggccatgtgc gccctcgacg agtggcaggt
     8041 cgacgacctg gcccggatcc tgcggcgcag cgccagcttc tcgaccctcg gcgacgaggt
     8101 cttccacgcc tgcctcgacc tgctggccgg ccgctacccg tccgaggagt tcagcgagct
     8161 gcggccccgc atcgtgtggg accgcaccgc gccgggtggg ggcggtggcg tcgtccgtgg
     8221 acgcgccggc gcgcagcgcc tcgccgtcac caaccccggc accatccccg atcgcgggct
     8281 cttcggggtg ttcctccccg acggcacccg ggtcggcgag ctcgacgagg agatggtgta
     8341 cgagtcccgg gccggggaga cgttcctact cggcgcgtcg acgtggcgca tcgaggagat
     8401 caccttcgag cgggtcatcg tcactcccgc tcctggcctg cccggcaaga tgccgttctg
     8461 gcacggcgag ggccccggcc gaccgctcga gctcggccag ggcatcggcc agttcgtgcg
     8521 cgagctgcgt gaccgcggca ccacgcccga ccccgtgaag accctgcggg aggaccacgg
     8581 tctcgacgag ctcgccgcgc tgaacctggt gtcgtacctg gacgagcagg ccgaggccac
     8641 cggtgtcgtc cccgacgacc gcaccatcgt ggtcgagcgc ttccgggacg agatcggcga
     8701 ctggcgcgtc tgcgtgctgt cgccgttcgg cgcccaggtg cacgccccgt gggccatggc
     8761 gctgcagcag acgctggggg agcggtgggg cgtcgacgtc gagatgatct ggtccgacga
     8821 cggcatcgtg ctgcgcctgc ccgaggcggt ggaggagctg cccgtcgagg agctggccat
     8881 cccgcccgac gagatccgcg acatcgtcct cggccagctg ccgaacacgg cgatgttcgc
     8941 gtcccgcttc cgcgagtgcg ccggccgtgc cctgctcctg ccccgccgcc ggcccgaccg
     9001 ccgccagccg ctgtggca
//
LOCUS       DATISU010000225         1777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_291368, whole genome shotgun sequence.
ACCESSION   DATISU010000225 DATISU010000000
VERSION     DATISU010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291368"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VLR27_09260"
     CDS             <1..322
                     /locus_tag="VLR27_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HSP03676.1"
                     /translation="WPQLLDATFPPGSITGAPKLAAMEVIRQLEPVGRGIYCGGDGWV
                     DADRGIGELNVAIRTFWIDPDGERGPTLRFGTGGGITHDSDPAGEWAETELKARTLLA
                     VASG"
     gene            complement(469..>1777)
                     /locus_tag="VLR27_09265"
     CDS             complement(469..>1777)
                     /locus_tag="VLR27_09265"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSP03677.1"
                     /translation="GIPTLINTAKARPGHPSALILSPTRELADQIKDELAPLAKAADR
                     TVTAVYGGVGYGPQTKAIRNGVDVLVACPGRLEDLVSSGSVILAEVGVVVVDEADRMA
                     DMGFLPAVKRLLDRTKTDRQTLLFSATLDGDIAELTRRYQKDPARHEVGEVEPDMTAM
                     AHHFWSIDHEARVDHTRDAVVAAGPTIVFTRTRHGADRLCKQLGKAGVEAAAIHGGRN
                     QNQRTRALEAFTKGQVQALIATDVAARGIHVDGVACVVHFDPPEDDKTYLHRSGRTAR
                     AGAQGVVVSFVRDNNRKDVKTLQRDLGLAQVVTTPDATLLGDGSRHREATSRQKTQPA
                     AKAQQSSPPKGGGGNGGRNRQRTRTRDGEDRAAGKSARRDDRPARDGGKPRTRTKPAE
                     RRGADGVVDVDPRPRKERQGSGGRSTPPSKVHRKGGGARALRG"
BASE COUNT          203 a          580 c          733 g          261 t
ORIGIN      
        1 ttggccgcag ctgctcgacg ccacgttccc gccgggttcg atcacgggcg cgccgaagct
       61 ggcggcgatg gaggtcatcc gccagctcga acccgtcggc cgcggcatct actgcggcgg
      121 cgacgggtgg gttgacgccg atcgggggat cggcgagctg aacgtcgcga tccggacgtt
      181 ctggatcgac ccggacgggg agcgggggcc caccctccgg ttcggcaccg ggggcgggat
      241 cacccacgac agcgacccgg ccggcgagtg ggccgagacc gagctcaagg cccggacgct
      301 gctggcggtc gcctccggct gaaggagccg ggacgaccgc cggagcgagc agcgggtgcg
      361 gggcgcctcg gtggcctgcg cccggagccg agccgccagg cgaggcgacc catgtgacgg
      421 cgcccggtgg cggcgaagcc gccacgacgg aagccgagct ggatgttcct agccgcgcag
      481 cgctcgtgcg ccgccaccct tgcggtgcac cttggacggc ggggtgctgc gaccgccgga
      541 accctggcgc tccttgcggg ggcgggggtc cacatcgacc acgccgtcgg cgccgcggcg
      601 ctcggccggc ttggtgcggg tgcggggctt gccgccgtcg cgggccggac ggtcgtcgcg
      661 gcgtgcgctc ttgccggcgg cacggtcctc gccgtcgcgg gtgcgggtcc gctggcggtt
      721 gcgaccgccg ttgccgccgc cgcccttggg cggggacgac tgctgggcct tggcggccgg
      781 ctgggtcttc tggcgggacg tggcctcgcg gtgacgggag ccgtcaccga ggagcgtggc
      841 gtccggggtg gtcacgacct gggcgagacc gaggtcgcgc tggagggtct tgacgtcctt
      901 gcggttgttg tcccgcacga aggacacgac cacgccctgg gcgccggcgc gagccgtgcg
      961 gccggagcgg tgcaggtagg tcttgtcgtc ctcgggcggg tcgaagtgca cgacgcaggc
     1021 cacgccgtcg acgtggatgc cgcgggcggc gacgtcggtg gcgatgagcg cctggacctg
     1081 acccttggtg aaggcttcga gggcccgggt gcgctggttc tggttgcgac cgccgtggat
     1141 ggccgcagcc tcgacgccgg ccttgcccag ctgcttgcag aggcggtcgg caccgtggcg
     1201 ggtgcgggtg aacacgatgg tcggcccggc ggccacgacg gcgtcacggg tgtggtcgac
     1261 gcgcgcctcg tggtcgatgg accagaagtg gtgcgccatt gcggtcatgt ccggctcgac
     1321 ctcgccgacc tcgtggcggg cggggtcctt ctggtagcgg cgggtcagct cggcgatgtc
     1381 accgtcgagc gtggccgaga acagcagggt ctggcggtcg gtcttggtgc gatcgagcag
     1441 gcgcttgacc gcggggagga accccatgtc ggccatgcga tcggcctcgt cgacgacgac
     1501 gacacccacc tcggcgagga tgacgctgcc gctgctgacg aggtcttcga gccggccggg
     1561 gcaggcgacg aggacgtcga cgccgttgcg gatggccttg gtctgggggc cgtagccgac
     1621 accgccgtac accgcggtga cggtgcggtc ggcggccttg gccagcgggg cgagctcgtc
     1681 cttgatctgg tcggccagct cgcgggtcgg cgacaggatc agggcgctgg ggtgcccggg
     1741 ccgggccttg gcggtgttga tcagggtcgg gatgccg
//
LOCUS       DATISU010000226         8129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_58478, whole genome shotgun sequence.
ACCESSION   DATISU010000226 DATISU010000000
VERSION     DATISU010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58478"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..962
                     /locus_tag="VLR27_09270"
     CDS             39..962
                     /locus_tag="VLR27_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSP03678.1"
                     /translation="MAEPRFDSVPEVREGLKKVDYLSDEGIAGIVYLADRLQKPILVE
                     GPAGTGKTQLAKSVAEMMGARLIRLQCYEGLDESKALYEWNYKKQLLRIQAAGKQDDS
                     GSDEWDALSGDIFSDDFLLTRPLLEAITADDPVVLLIDEVDRVEVETEALLLEILSDY
                     TVSIPELGTIEAKQIPMVFLTSNNTRELSEALKRRCLYLHVDYPDLEREKEIVLTKVP
                     DITESLADQVARIVRSIRNLELKKSPSVSETLDWARTLLLLGVNQIDAETARSTINIL
                     LKYQSDITKALRELEDAKSDMAKDTTTAPSS"
     gene            974..1471
                     /locus_tag="VLR27_09275"
     CDS             974..1471
                     /locus_tag="VLR27_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSP03679.1"
                     /translation="MGDPTGVLALTDGLPARHLAAGEVLFGQGDGDERTVAVLVEGSL
                     RVELDGVALAEITVPGAFVGEIGALLGTARTATVVAVEPSEVRVIGDPESFFRDDPRL
                     GLEVARQLAGRLQRLLAYLGDVRAQYAGADGHLGVLDTVLGKIASRPPVDIEPGSDRD
                     ADYDA"
     gene            complement(1459..2196)
                     /locus_tag="VLR27_09280"
     CDS             complement(1459..2196)
                     /locus_tag="VLR27_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSP03680.1"
                     /translation="MRALLVSDLHYDLRKLDWVLAEADDVDLLAIAGDLLDVGSALPL
                     DAQIAVVLEYLARMAARTTTVVCSGNHDLDHRTAEGEKATAWIAEARAGGVVVDGDSA
                     EVDGWLVTACPWWEGPHSLARMEADLADAAEQRQGPWLWVYHGPPEGPLSWTGSRHFG
                     DPELPRLLDQHHPDIVLCGHIHQAPYVDGGAWFEQRADTWLFNSGFQTGPIPAHVLLD
                     LSASRAEWWSLQGQGTIDLDLGDAQAS"
     gene            2285..3796
                     /locus_tag="VLR27_09285"
     CDS             2285..3796
                     /locus_tag="VLR27_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSP03681.1"
                     /translation="MTPRPPTETDTDPELELGAGEAISELLAGFIQELREAGLPVSLT
                     ENLDAMEAVKHIPLEDREAFKYALAATLVKNNAHWRAFETVFEVYFSYRGPEYELDPD
                     SDLAQAIDELMEQLQGDQGEDGDQSGGGGAMDAMTPEEIAQMLMKALQQGDNGMMRAV
                     ARQSVKRFAGMEPGRPVGGTYYLYRTLRHLDLDNLLEQLMEQARQQSPEPLSQLEERL
                     ERDEYETRIEKLKQEIESEIRRRLVADRGVEAMARTLRKPLPEDIDFMHASREEMVVL
                     RKALQPLARKLAVRLARKRRHGRKGPLDFRSTIRHSLSYGGVPAEPKFRYPRPSKPEI
                     FVVADISGSVASFARFTLHLVYAIQGQFSKVRAFVFIDGIDEVTHLFEGVEDITEAVH
                     RVNTEADVVWVDGHSDYGHAFEVFWSRYGKDVGPKTTVLLLGDARNNYHASQSWVVKD
                     MQHRARHVYWLNPEPKSYWDTGDSIIGEYAGYTDGVYEVRNLRQLERFVDILE"
     gene            4012..5190
                     /locus_tag="VLR27_09290"
     CDS             4012..5190
                     /locus_tag="VLR27_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HSP03682.1"
                     /translation="MLGLLAGSFAASLAAPQAARADTASERAEVQRKRAAVASEIDTL
                     RATNAQVEAALSALNDNVATQTAALRDAERRSAEAQAELTRATELVVAKQAEIAALDA
                     AVKDLAVETFIRPPSEASLVDSLESETIGEAELKQSLLEAKSSSQLDALDQLDKARED
                     LEIAQAAAEEAAVVADREQAAVDARLDEVTVARDQQAKVVAEVESRLDRQLAEAAVLD
                     QQDQALGAKLAAEQAALAKRLAAQRAAAAAAQRSSGPVTISITGSGSIVSVRGIRIHQ
                     SIADNLAGLLSAAESDGISLSGWGYRDSQRQVELRRQNCGSSNYAVYQMSPSACSPPT
                     ARPGASNHERGLAVDFTYNGSTIKSRSSPAFQWLRANAARYGFYNLPSEPWHWSVNGN
                     "
     gene            complement(5316..6194)
                     /locus_tag="VLR27_09295"
     CDS             complement(5316..6194)
                     /locus_tag="VLR27_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSP03683.1"
                     /translation="MTEQRTVTSADGLRLAVWEHGDPTRPTVVLIHGYPDTHRVWDGV
                     VAELSGTHHVVTYDVRGAGASGIPGDRAGYDLDRLSEDLVAVVDATCPDGPVHVAGHD
                     WGSIQGWEAVCTGALGDRLATYTSMSGPSLDHVGHWLRSRRAGEGGGRRELLRQGLHS
                     WYVAFFQLPVLAPFGWRRFAERGFRRYLEKVEGLGPDAQPGPTLSEDGANGVELYRQN
                     VRSRVGHPRERRADVPVLLIVATGDRYVTTALLDDVGQHCSDLRRVDVDARHWLPAAQ
                     PDVVASLVAEHVARHG"
     gene            complement(6229..6918)
                     /locus_tag="VLR27_09300"
     CDS             complement(6229..6918)
                     /locus_tag="VLR27_09300"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HSP03684.1"
                     /translation="MSVTLPFPSQRHSYTDDELVEVNAELEHAPASKVIAWAAEAFGP
                     HLCLTASMNDAVLIDLAVKVDPAIEVVFIDTGYHFPETLATVEAVRRRYGLNMKIMTA
                     PENTELLWKTDPDNCCSALKVQQLDRALAGKAAWMSGIRRAEADTRATAHVVGRDLRG
                     LVKVNPIAQWTDADVQAYIEAHDVPVNPLLAQGYGSIGCMPCTEKVAAGEDARAGRWR
                     GKSKTECGLHG"
     gene            complement(6974..>8129)
                     /locus_tag="VLR27_09305"
     CDS             complement(6974..>8129)
                     /locus_tag="VLR27_09305"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HSP03685.1"
                     /translation="VFVAGGLGANPHPALALEEFTPRHRLLETIEAILRVFNNHGFRD
                     NKLRARMKWLVDTMGWEELQGRILKERAFLVASSSWPGGVPELVEKHGDTAAGVAAGT
                     APTPVGQGVPVMLRRRGAYEAWEDANVVRGNAKGTVSAYAWSRLGDVTSEQFRALAQI
                     QRDLDAEVRITNRQNLVYRGLSEEQLPVLFDRLAAIGMAEPGAELSRDVVACPGADTC
                     NLAVTQSRGLASAIGEALEEAGLAEVGGVRTNISGCTNSCGQHHISDIGFHGVERRAN
                     GRSAPGYQMLLGGYVGQEQIHFGDKALRLPAKNAPEAAVRVVRRFAAERQAGEDFRAW
                     MDRAGGAKAIAEDLKELDAFPDYEEAPDFYVDYGETGPYVKEIGESECAT"
BASE COUNT         1223 a         2933 c         2847 g         1126 t
ORIGIN      
        1 gttgaccgag cggtcaagac ggccggtagg gtcgggagat ggccgaaccg cgcttcgatt
       61 ccgtccccga ggtccgcgag ggcctgaaga aggtcgacta cctctccgac gagggcatcg
      121 ccggcatcgt ctacctcgcc gaccgcctgc agaagccgat cctcgtcgag ggtccggccg
      181 gcacgggcaa gacccagctg gccaagtcgg tcgccgagat gatgggcgcc cggctgatcc
      241 ggctgcagtg ctacgagggc ctggacgagt ccaaggcgct gtacgagtgg aactacaaga
      301 agcagctgtt gcgcatccag gccgccggca agcaggacga ctccggcagc gacgagtggg
      361 atgcgctgtc gggtgacatc ttctccgacg acttcctcct cacccgtccg ctgctcgagg
      421 ccatcaccgc cgacgacccc gtcgtcctgc tgatcgacga ggtcgaccgc gtcgaggtcg
      481 agaccgaggc cctcctgctc gagatcctgt cggactacac cgtctcgatc cccgagctcg
      541 gcaccatcga ggccaagcag atcccgatgg tcttcctcac ctccaacaac acccgtgagc
      601 tgtccgaggc cctgaagcgg cgctgcctct acctgcacgt cgactacccg gacctcgagc
      661 gcgagaagga gatcgtgctc accaaggtgc cggacatcac cgagtccctc gccgaccagg
      721 tcgcccgcat cgtccgctcg atccgcaacc tcgagctgaa gaagagcccg tcggtctccg
      781 agacgctcga ctgggcccgc accctgctgc tcctcggcgt caaccagatc gatgccgaga
      841 ccgcccggag caccatcaac atcctgctga agtaccagtc cgacatcacc aaggcgctgc
      901 gcgagctcga ggacgccaag tccgacatgg ccaaggacac caccaccgca cccagcagct
      961 gacggggccg gtcgtgggcg accccaccgg tgtccttgcc ctgaccgacg gcctgccggc
     1021 gcgccacctg gcggcgggcg aggtcctctt cggtcagggg gacggtgacg agcggacggt
     1081 cgccgtgctc gtcgagggct cgctccgcgt cgagctcgac ggcgtggcgc tggctgagat
     1141 caccgtgccc ggcgccttcg tcggcgagat cggtgccctc ctcggcaccg cccggaccgc
     1201 caccgtcgtg gccgtcgaac cgtccgaggt gcgcgtcatc ggcgatccgg agtcgttctt
     1261 ccgggacgat ccgcgcctcg gcctcgaggt cgcccgtcag ctcgccggcc ggttgcagcg
     1321 gctcctggcg tacctgggcg acgtccgggc ccagtacgcc ggcgccgacg gtcacctcgg
     1381 cgtgctcgac accgtcctcg gcaagatcgc ctcgcgcccc ccggtcgaca tcgagccggg
     1441 ctccgaccgc gacgccgact acgacgcctg agcgtcgccg aggtcgaggt cgatcgtgcc
     1501 ctggccctgg agcgaccacc actcggcccg actcgccgac aggtcgagca gcacgtgggc
     1561 ggggatcggg ccggtctgga agccgctgtt gaagagccag gtgtccgcgc gctgctcgaa
     1621 ccacgccccg ccgtcgacgt agggcgcctg gtggatgtgg ccgcacagga cgatgtcggg
     1681 gtggtgctga tccagcaggc gggggagctc gggatcgccg aagtgtcgcg acccggtcca
     1741 cgagagggga ccctccggtg ggccgtggta cacccacagc cacggcccct ggcgctgctc
     1801 ggcggcgtcg gcgaggtcgg cctccatgcg ggcgaggctg tggggtccct cccaccaggg
     1861 gcacgccgtg accagccagc cgtcgacctc ggcggagtcc ccatcgacga ccacgccgcc
     1921 ggcccgggcc tctgcgatcc acgccgtcgc cttctcgccc tccgcggtgc ggtggtccag
     1981 gtcgtggttc cccgagcaca cgacggtggt ggtccgagcc gccatgcggg cgaggtactc
     2041 gaggacgacg gcgatctggg cgtcgagcgg cagggctgac cccacgtcga ggaggtcgcc
     2101 ggcgatcgcc aggaggtcga cgtcgtccgc ctcggccagc acccagtcga gcttgcgcag
     2161 gtcgtagtgg aggtccgaga ccagcagcgc ccgcacggcg ggagtctggc acggtcccgt
     2221 ctccggtgga cgccgccccg gatccattga ccggacggtc aagtaccacg actaccctgg
     2281 gcccgtgacc ccgcgacccc cgacggagac ggacaccgac ccggagctgg agctgggcgc
     2341 cggcgaggcg atcagcgagc tgctggccgg gttcatccag gagctgcgtg aggccggcct
     2401 gccggtcagc ctcaccgaga acctggatgc catggaggcg gtcaagcaca tccccctcga
     2461 ggaccgcgag gcgttcaagt acgcgctcgc cgccacgctc gtgaagaaca acgcgcactg
     2521 gcgcgccttc gagaccgtgt tcgaggtgta cttcagctac cggggccccg agtacgagct
     2581 cgaccccgac agcgacctgg cccaggccat cgacgagctc atggagcagc tccaggggga
     2641 ccagggcgag gacggcgacc agtcgggcgg gggtggcgcc atggacgcca tgacgcccga
     2701 ggagatcgcc cagatgctga tgaaggcgct ccagcagggc gacaacggca tgatgcgcgc
     2761 cgtcgcccgc cagtcggtga agcgcttcgc cggcatggag ccgggtcgcc cggtcggcgg
     2821 cacctactac ctgtaccgca cgctgcggca cctcgatctc gacaacctgc tcgaacagct
     2881 catggagcag gcccgccagc agagccccga gccgctgagc cagctcgagg agcgcctcga
     2941 gcgcgacgag tacgagaccc gcatcgagaa gctgaagcag gagatcgagt ccgagatccg
     3001 ccggcgcctg gtggccgacc ggggcgtgga ggcgatggcc cgcacgctgc gcaagccgct
     3061 gcccgaggac atcgacttca tgcacgcctc ccgcgaggag atggtggtgc tgcgcaaggc
     3121 gctgcagccc ctcgcccgca agctcgccgt gcgcctggcc cgcaagcgcc gccacggccg
     3181 caagggcccc ctcgacttcc gcagcaccat ccgccactcg ctgtcctacg gcggcgtgcc
     3241 ggccgagccc aagttccgct acccccggcc gtccaagccc gagatcttcg tggtggccga
     3301 catctccggt tcggtcgcgt ccttcgcccg cttcaccctg cacctcgtgt acgccatcca
     3361 ggggcagttc tccaaggtcc gggccttcgt gttcatcgac ggaatcgacg aggtcaccca
     3421 cctcttcgag ggcgtcgagg acatcaccga ggccgtgcac cgggtcaaca ccgaggccga
     3481 cgtggtctgg gtcgacgggc actccgacta cgggcacgcc ttcgaggtct tctggagccg
     3541 ctacggcaag gacgtcggcc ccaagaccac cgtgctgctg ctcggcgacg cccggaacaa
     3601 ctaccacgcg tcccagtcct gggtcgtcaa ggacatgcag caccgggccc gtcacgtgta
     3661 ctggctcaac cccgagccga agtcgtactg ggacaccggc gactcgatca tcggggagta
     3721 cgccgggtac accgacgggg tgtacgaggt gcgcaacctg cgccagctcg agcgcttcgt
     3781 cgacatcctc gagtaggcag cccgctcggc acttcccgcg cagggtcgcg ctcgcgcgag
     3841 caggaggtgg ctacggtgtt gccgaagtgt gacggagccg tcgcgttccg ttaccctttt
     3901 gcaacactcg tcagaccggt ttgtccggtc cggccgccct gaccccacca ggacccaccc
     3961 caccccatgc atcaccaccg actccgccgc tccgtcggcc ccgttctcgc tgtgctcggc
     4021 ctcctggccg gatcgttcgc cgcgtcgctc gcggcacccc aggccgctcg ggccgacacc
     4081 gcctcggagc gggccgaggt ccagcgcaag cgggccgccg tcgcatccga gatcgacacg
     4141 ctccgggcca cgaacgccca ggtcgaggcg gccctgtccg cgctcaacga caacgtcgcc
     4201 acccagaccg ccgccctgcg cgacgccgag cgccgctcgg ccgaggctca ggccgagctg
     4261 acccgggcca ccgagctggt ggtcgccaag caggccgaga tcgccgccct cgacgccgcg
     4321 gtcaaggacc tggcggtcga gaccttcatc cgaccgcctt cggaggcgag cctcgtggac
     4381 tccctcgagt ccgagacgat cggcgaggcc gagctcaagc agtccctcct cgaggccaag
     4441 tcgtcgtcgc agctcgacgc cctcgatcag ctggacaagg cgcgcgagga cctcgagatc
     4501 gcccaggccg ccgccgagga ggccgccgtg gtcgccgacc gggagcaggc cgccgtcgac
     4561 gcccgcctcg acgaggtcac cgtggcccgt gaccagcagg ccaaggtcgt cgccgaggtc
     4621 gagtcccgcc tcgatcgcca gctcgccgag gccgccgtcc tcgatcagca ggatcaggcg
     4681 ctgggagcca agctcgccgc cgagcaggcc gcgctggcca agcgcctcgc agcgcagcga
     4741 gccgcagctg ccgccgccca gcgatccagc ggcccggtca cgatcagcat caccggctcg
     4801 ggcagcatcg tgtcggtgcg gggcatccgc atccaccaga gcatcgccga caacctcgcc
     4861 ggtctcctgt ccgccgccga gtccgacggc atcagcctca gtggctgggg ctaccgggac
     4921 tcccagcgcc aggtcgagct ccgccgccag aactgcggct cgagcaacta cgccgtgtac
     4981 cagatgtccc cgagcgcctg cagcccgccc acggcccggc ccggcgcctc gaaccacgag
     5041 cgcggcctcg ccgtcgactt cacctacaac ggcagcacca tcaagtcccg cagctcaccg
     5101 gcgttccagt ggctgcgagc caacgccgcc cgctacggct tctacaacct gcccagcgag
     5161 ccctggcact ggtcagtcaa cggcaactga gcaccagcgc aacacggcaa gaaactggcc
     5221 gccgaatcgt cacgcagccc cgcagggggt tgtgtgacga ggcggctgcc agtttccaga
     5281 gtcggaacac aaggggcagc tggagcagtc gccagctacc cgtgccgggc gacgtgctct
     5341 gcgacgagcg atgccacgac gtccggctgc gccgccggca gccagtggcg ggcgtcgacg
     5401 tccacccggc gcaggtccga gcagtgctgg ccgacgtcgt cgagcagggc ggtggtgacg
     5461 tagcggtcac cggtcgccac gatgagcagg accgggacgt cggcgcgtcg ctcccgggga
     5521 tggccgaccc gtgaccgaac gttctggcgg tacagctcca cgccgttggc cccgtcctcg
     5581 gacagggtcg gccccggctg ggcgtcggga ccgaggccct ccaccttctc caggtaccgg
     5641 cggaagcccc gctcggcgaa ccggcgccac ccgaacgggg ccagcaccgg cagctggaag
     5701 aaggcgacgt accacgagtg cagtccctgg cggagcagct cccgtcgacc gccgccctcg
     5761 ccggcgcggc gggagcggag ccagtgcccg acgtggtcga ggctcggccc ggacatcgac
     5821 gtgtacgtgg ccaggcggtc gccgagggcg ccggtgcaga ccgcctccca gccctggatc
     5881 gagccccagt cgtgtccggc gacatgcacg ggaccgtccg ggcatgtcgc gtcgacgacg
     5941 gcgacgagat cctcgctcag acggtccagg tcgtagccgg cccggtcgcc ggggatcccg
     6001 gaggcaccgg cgccacggac gtcgtaggtg acgacgtggt gggtgccgga gagctcggcc
     6061 acgacgccgt cccacacccg gtgggtgtcg gggtagccgt ggatcagcac gacggtcggg
     6121 cgtgtggggt cgccgtgctc ccagacggcc agccggaggc cgtcggcgct ggtgacggtg
     6181 cgttgctcgg tcatggcggg accgtaccca ggtcgccgac gaggagactc agccgtggag
     6241 gccgcactcc gtcttcgact tgccccgcca gcggccggcg cgggcgtcct cgcctgccgc
     6301 gaccttctcg gtgcagggca tgcagccgat ggagccgtag ccctgggcca gcagggggtt
     6361 gaccggtacg tcgtgggcct cgatgtaggc ctgcacgtcg gcgtcggtcc actgggcgat
     6421 tgggttgacc ttcaccaggc cccgcaggtc gcggcccacg acgtgggcgg tcgcccgggt
     6481 gtcagcctcg gcccggcgga tgccgctcat ccacgccgcc ttgccggcga gggcccggtc
     6541 gagctgctgg accttcagcg cggagcagca gttgtcgggg tcggtcttcc agagcagctc
     6601 ggtgttctcc ggcgccgtca tgatcttcat gttcagcccg taccggcgcc ggacggcctc
     6661 gaccgtggcc agcgtctcgg ggaagtggta gccggtgtcg atgaagacca cctcgatggc
     6721 cgggtcgacc ttgaccgcca ggtcgatcag gacggcgtcg ttcatcgacg ccgtgaggca
     6781 gaggtggggg ccgaaggcct cggcggccca ggcgatgacc ttggaggcgg gggcgtgctc
     6841 gagctcggcg ttgacctcga cgagctcgtc gtcggtgtag gagtggcgct ggctggggaa
     6901 cgggagggtg acgctcatcg gtgcgggggc ttccgggtcg gagggggcag gtgggggaga
     6961 ggtggcgtgg cgctcaggtg gcgcactcgg actcgccgat ctccttcacg tagggcccgg
     7021 tctccccgta gtcgacgtag aagtccgggg cctcctcgta gtccgggaac gcatcgagct
     7081 ccttcaggtc ctcggcgatc gccttggccc cgccggcgcg atccatccag gcgcggaagt
     7141 cctcacccgc ctggcgctcc gcggcgaacc ggcgcacgac gcgcaccgcc gcctctgggg
     7201 cgttcttggc cggcaggcgc agcgccttgt cgccgaagtg gatctgctcc tggcccacgt
     7261 agccaccgag cagcatctgg tagccggggg cggagcggcc gttggcccga cgctccacac
     7321 cgtggaagcc gatgtcggag atgtggtgct ggccgcagga gttggtgcac ccggagatgt
     7381 tcgtccggac gccaccgacc tcggcgagac cggcttcctc gagggcctcg ccgatggcgc
     7441 tggccagccc gcgggactgg gtgaccgcga ggttgcaggt gtcggcgccg gggcaggcca
     7501 cgacgtcgcg cgacagctcg gcgccgggct cggccatgcc gatggcggcg agccggtcga
     7561 acaggacggg cagctgctcc tcggagaggc cgcggtacac caggttctgg cggttggtga
     7621 tgcgcacctc ggcgtcgagg tcgcgctgga tctgggccag ggcccggaac tgctcggagg
     7681 tgacgtcgcc gaggcgggac cacgcgtagg ccgagacggt gcccttggcg ttgccccgga
     7741 cgacgttggc gtcctcccag gcctcgtagg cgccgcggcg gcgcagcatg accggcacgc
     7801 cctggccgac gggggtgggg gcggtgccgg cggcgacgcc ggctgcggtg tcgccgtgct
     7861 tctcgaccag ctcgggcacg ccgcccggcc acgaggagga ggccacgagg aaggcgcgct
     7921 ccttgaggat gcggccctgc agctcctccc agcccatcgt gtccaccagc cacttcatgc
     7981 gggcccgcag cttgttgtcg cggaagccgt ggttgttgaa cacccgcagg atggcctcga
     8041 tcgtctcgag gaggcggtgc cggggggtga actcctcgag ggcgagggcg gggtgggggt
     8101 tggcgccgag gccgccagcc acgaacacc
//
LOCUS       DATISU010000227         4841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_617492, whole genome shotgun sequence.
ACCESSION   DATISU010000227 DATISU010000000
VERSION     DATISU010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617492"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VLR27_09310"
     CDS             complement(<1..274)
                     /locus_tag="VLR27_09310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HSP03686.1"
                     /translation="MSTEHTLDHVTSSPARAEPGSDAMFRAIVQTSVNPFVVLDTVGH
                     VRWCSEKVETLIGRTSEDVIGRHFLDLIDPSSHEAVLAEYAEFTSDD"
     gene            complement(354..839)
                     /locus_tag="VLR27_09315"
     CDS             complement(354..839)
                     /locus_tag="VLR27_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HSP03687.1"
                     /translation="MLDDTLLATARHAGDLLVRRGETVGVAEGSCGGLVSAALLAVPG
                     ASAYYRGGAVIYTAAAVRGLMGDAVPRPEPLRGASEPFARWLAEEISDDLGATWGIGE
                     GGATGPSGNPYGDPPGHCWTAVHGPAPATRHLLTGDDDRLANMTAFATAALTLLVDQL
                     G"
     gene            979..1542
                     /gene="ahpC"
                     /locus_tag="VLR27_09320"
     CDS             979..1542
                     /gene="ahpC"
                     /locus_tag="VLR27_09320"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HSP03688.1"
                     /translation="MSVLSTEIKPFQTTAFHNGEFVPVSDDDVRGRWAIFFFYPADFT
                     FVCPTELGDLADHYDELASLGVEVYSVSTDTHFVHKAWHGSSETIGKIRYPMLGDPTG
                     TIARNFDNMREDEGVADRGTFLVDPDGVIQLVEITPEGIGRNAAELLRKVKAAIYVRK
                     HPNEVCPAKWEEGAETLAPSLDLVGKI"
     gene            1663..3246
                     /gene="ahpF"
                     /locus_tag="VLR27_09325"
     CDS             1663..3246
                     /gene="ahpF"
                     /locus_tag="VLR27_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HSP03689.1"
                     /translation="MLDTALADQLRAHLTNVTMPVELVASLDDSAKSAELAELLDEIA
                     GMSDLLTLRTDGDDARRPSFAITRAGTDVRVDFAGVPMGHEFTSLVLALLQVGGHPPK
                     VDPDTAEAIRLLDGEHRFETYFSLSCQNCPDVVQALNVLSVLNPSFSHTAIDGAVFPD
                     EAEEREVLSVPAVFLDGELFAQGRMDLEELLAKLDTGSAERAAEEIDAKDPFDVLVVG
                     GGPAGAAAAVYASRKGIRTGLVAERFGGQVGDTMGIENLISVPYTEGPKLVAALEAHV
                     GEYDVDVMNLQRAARLVPASGEGGLHSVVLDSGATVRARTVVVSTGARWRTLGVPGED
                     RFRNKGVHFCPHCDGPFYKGKRIAVVGGGNSGVEAAIDLAGIVGHVTVLEFTDRLLAD
                     EVLQRKLHSLPNVDVVLEAQTTELTGDAKMEGLTYIDRRTGDERRLDVDGVFVQIGLL
                     PNTEWIDGTVELSDRNEVVIDERGRTDIPGVFAAGDCTTVPYKQIVVSLGAGSTAALS
                     AFDHLIRTSAPAGEMAGAS"
     gene            complement(3315..3785)
                     /gene="bfr"
                     /locus_tag="VLR27_09330"
     CDS             complement(3315..3785)
                     /gene="bfr"
                     /locus_tag="VLR27_09330"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HSP03690.1"
                     /translation="MQGDPEIIEFLNEALTAELTAINQYFIHAKMCENWGYKRLAKHN
                     YDESIDEMKDAEKVIERILYLDGVPNLQRLNSVLVGETVPEQHRLDLDLEKAAVDRYN
                     RGVALAREKGDNGTAELLEHLLVGEEEHADWLESQLNVIDQVGLENYLSQHIHD"
     gene            complement(3821..4015)
                     /locus_tag="VLR27_09335"
     CDS             complement(3821..4015)
                     /locus_tag="VLR27_09335"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSP03691.1"
                     /translation="MIVCHCHAVRAEEIRTEVRLGAETVEVVGARCGAGTRCGGCIPA
                     LEAVIADEQARMRDAVPAPH"
     gene            complement(4012..4254)
                     /locus_tag="VLR27_09340"
     CDS             complement(4012..4254)
                     /locus_tag="VLR27_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03692.1"
                     /translation="MSAPVQELPVAERERAAAAVRDRFDRILWQRLELRDGGLCAVVA
                     GTRHRRPRQIHVGVAAALGLLDQGVPTVGPRVGSRS"
     gene            4411..>4841
                     /locus_tag="VLR27_09345"
     CDS             4411..>4841
                     /locus_tag="VLR27_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03693.1"
                     /translation="MRQLRLTAPDGARDRLDGLLDRHDAHAVRIWDLDGDATCVELSL
                     TNRDIGDFLDDVDDLGDVTITMAPQSVLTLVPGRTEAPQDLVDVEPLSPHEVFLAGLQ
                     SVGSWTGFLGYAAVAGAVVWIGMQLNSVFLLIAAMLIAPFAG"
BASE COUNT          686 a         1769 c         1672 g          714 t
ORIGIN      
        1 ggtcgtcgga cgtgaactcg gcgtactcgg ccagcactgc ctcgtgcgac gacgggtcga
       61 tgaggtcgag gaagtggcgc ccgatgacgt cttccgaggt ccggccgatg agcgtctcca
      121 ccttctcgct gcaccaacgc acgtgcccca cggtgtcgag cacgacgaag gggttgaccg
      181 acgtctgcac gatcgcccgg aacatggcgt cgctgcccgg ctccgcgcgc gccggcgacg
      241 aggtcacgtg atccagcgtg tgctccgtgc tcatgaggtg ccctactcgc gcgccgactg
      301 caccgcgcaa cctaggcggc agccgctgat tccgtccggt cggagggtgg gcgtcagccg
      361 agctggtcga cgagcagggt gagcgcggcg gtggcgaacg cggtcatgtt cgcgaggcgg
      421 tcgtcgtcgc cggtgagcag gtgccgggtg gccggcgccg ggccgtggac cgccgtccag
      481 cagtgcccgg gcgggtcgcc gtaggggttc ccggagggcc cggtcgcccc gccctcgccg
      541 atgccccacg tcgcgcccag atcgtcgctg atctcctcgg cgagccaccg ggcgaagggc
      601 tcgctggcgc ctcgcagcgg ttcgggccgg ggcacggcat cgcccatgag gccgcgtacg
      661 gcagccgcgg tgtagatcac cgcgccgccc cggtagtagg cggacgcccc gggcacggcc
      721 aggagggccg cggagaccag ccccccgcag ctgccctcgg cgacgccgac ggtctcgccc
      781 cggcgcacga ggaggtcgcc ggcgtggcga gcggtggcga gcagcgtgtc gtcgagcacc
      841 gccggactct agggcgccga tcggcatagg agatggttat gggtctgata agtgccatct
      901 gtttggggca cgtcaaggcc gtccgtatgg tcacccgagt ccccctgcaa gacgacgatt
      961 cccggaggaa ccgtttccgt gtccgtgctc agcaccgaga tcaagccctt ccagaccacc
     1021 gccttccaca acggcgagtt cgtgcccgtc agcgacgacg acgtgcgggg ccggtgggcg
     1081 atcttcttct tctacccggc cgacttcacc ttcgtctgcc ccaccgagct cggtgacctc
     1141 gccgaccact acgacgagct ggcctccctc ggggtcgagg tgtactcggt ctccaccgac
     1201 acccacttcg tgcacaaggc gtggcacggg tcgtcggaga ccatcggcaa gatccggtac
     1261 ccgatgctcg gtgaccccac cggcaccatc gcccggaact tcgacaacat gcgagaggac
     1321 gagggcgtcg ccgaccgcgg caccttcctc gtcgatcccg acggcgtcat ccagctcgtc
     1381 gagatcaccc cggagggcat cggccgcaac gccgccgagc tcctccgcaa ggtgaaggcc
     1441 gccatctacg tgcgcaagca cccgaacgag gtctgcccgg ccaagtggga ggagggcgcc
     1501 gagacgctcg ccccctcgct cgacctcgtc ggcaagatct gaggcgcggg cgccccgcca
     1561 cgccccctca gacgttctcg acgcgcagat cgactcccca ccgtcgatct gcgcgtcggg
     1621 aacacctccc cctcgctcgc cagccccctg cctggagaac ccatgctcga caccgccctc
     1681 gcggaccagc tccgcgccca cctgaccaac gtgaccatgc cggtcgagct cgtcgcctcg
     1741 ctcgacgaca gcgccaagtc ggccgagctc gccgagctgc tcgacgagat cgccggcatg
     1801 tcggacctcc tcaccctgcg caccgacggg gacgacgccc gtcggccgtc cttcgccatc
     1861 acccgggccg gcaccgacgt ccgcgtcgac ttcgccggcg tgccgatggg gcacgagttc
     1921 acctccctgg tgctcgcgct gctccaggtc ggtggccacc cgcccaaggt cgaccccgac
     1981 acggcggagg ccatccggct cctcgacggc gagcaccggt tcgagacgta cttctcgctg
     2041 tcgtgccaga actgccccga cgtggtgcag gccctgaacg tgctcagcgt cctcaacccg
     2101 agcttctccc acaccgccat cgacggcgcc gtgttccccg acgaggcgga ggagcgagag
     2161 gtcctgtccg tccccgcggt gttcctcgac ggcgagctgt tcgcccaggg gcgcatggac
     2221 ctcgaggagc tgctggcgaa gctcgacacc ggcagcgccg agcgcgccgc cgaggagatc
     2281 gacgccaagg acccattcga cgtgctcgtg gtcggcggcg gaccggccgg tgccgctgcg
     2341 gccgtgtacg cgtcgcgcaa gggcatccgc accggcctcg tcgccgagcg ctttggcggt
     2401 caggtcggcg acaccatggg catcgagaac ctcatctccg tcccctacac cgagggcccc
     2461 aagctggtgg ccgcgctcga ggcccacgtc ggcgagtacg acgtcgacgt catgaacctc
     2521 cagcgggccg cccggctcgt gcccgcctcg ggcgagggtg gcctgcactc ggtggtgctc
     2581 gacagcggcg ccaccgtccg ggcccgcacg gtggtggtct ccaccggtgc ccgctggcgc
     2641 acgctcgggg tcccgggcga ggaccggttc cggaacaagg gcgtgcactt ctgcccccac
     2701 tgcgacggac cgttctacaa gggcaagcgc atcgcggtcg tcggcggcgg caactccggc
     2761 gtggaggccg ccatcgacct ggccggcatc gtcggccacg tcacggtgct cgagttcacc
     2821 gaccgcctgc tggctgacga ggtgctgcag cgcaagctgc actcgctgcc caacgtcgac
     2881 gtcgtgctcg aggcccagac caccgagctc accggcgacg ccaagatgga ggggctcacc
     2941 tacatcgatc ggcgcaccgg cgacgagcgg cgcctcgacg tcgacggcgt cttcgtccag
     3001 atcggcctgc tccccaacac ggagtggatc gacggcaccg tcgagctgtc cgaccgcaac
     3061 gaggtcgtga tcgacgagcg cggccgcacg gacatcccgg gcgtgttcgc cgccggcgac
     3121 tgcaccaccg tgccctacaa gcagatcgtg gtctcgctcg gggcggggtc gaccgcggcg
     3181 ctgtccgcct tcgaccacct gatccgcacc tcggcgccgg ccggggagat ggccggcgcc
     3241 agctgatccc gctcccggtc cggcgttccg tggccgctgc caggacgatc tggcagcggc
     3301 tgcggaacag cgactcagtc gtggatgtgc tggctcaggt agttctcgag cccgacctgg
     3361 tcgatgacgt tgagctggga ctcgagccag tcggcgtgct cctcctcgcc gacgagcagg
     3421 tgctcgagca gctcggccgt gccgttgtca cccttctccc gggcgagggc gacgccgcgg
     3481 ttgtagcggt cgaccgcggc cttctcgagg tcgaggtcga ggcggtgctg ctcgggcacg
     3541 gtctcgccca ccagcacgct gttgagccgc tgcaggttcg ggacgccgtc gaggtacagg
     3601 atccgctcga tgaccttctc ggcgtccttc atctcgtcga tggactcgtc gtagttgtgc
     3661 ttcgccaggc gcttgtagcc ccagttctcg cacatcttgg cgtggatgaa gtactggttg
     3721 atcgccgtga gctcggcggt gagcgcctcg ttcaggaact cgatgatctc ggggtcgccc
     3781 tgcacgggag cctcctggtc gtagcgacgg gtcgggacgg tcagtgtggc gcagggacgg
     3841 cgtcgcgcat gcgggcctgc tcgtccgcga tcacggcctc gagggccggg atgcaaccgc
     3901 cgcaccgggt gccggcaccg caccgggcgc cgacgacctc gacggtctcg gccccgagcc
     3961 gcacctcggt ccggatctcc tcggcacgca ccgcgtggca gtggcagacg atcacgaccg
     4021 gctccccacc cgggggccca ccgtgggcac accctggtcg agcagcccga gggcggcagc
     4081 gacgccgacg tggatctggc ggggccggcg gtgccgggtg ccggcgacca cggcgcacag
     4141 cccgccgtcg cgcagctcga ggcgctgcca caggatccgg tcgaagcgat cgcgcacggc
     4201 ggccgcggcg cgctcccgtt cggcgacggg gagctcctgc acgggtgcgg acacgacgtc
     4261 atcgtggcac gccttgccac cgtgtgtaaa gaggtcctaa cccacagtca tcgcccgccc
     4321 tgccggggcc tcggggcctc ggggtctgga gtccgggggc cgagggcagg aacgggtcgt
     4381 gaccgaagcg ctgacaggag cgagacgacc gtgcgccagc tgcgcctgac cgcccccgac
     4441 ggggcgcggg accggctcga cggcctgctc gaccgacacg acgcgcacgc cgtgcgcatc
     4501 tgggacctcg acggcgacgc cacctgcgtg gagctgtcgc tcaccaaccg ggacatcggc
     4561 gacttcctcg acgacgtcga cgacctcggc gacgtgacga tcacgatggc gccccagagc
     4621 gtgctcaccc tggtgccggg gcgcacggaa gcgccccagg acctcgtgga cgtggagccc
     4681 ctgagcccgc acgaggtctt cctcgccggg ttgcagtcgg tgggctcgtg gaccgggttc
     4741 ctcggctacg ccgcggtggc cggtgcggtc gtgtggatcg gcatgcagct gaactcggtc
     4801 ttcctgctga tcgccgcgat gctcatcgcg ccgttcgccg g
//
LOCUS       DATISU010000228         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_710481, whole genome shotgun sequence.
ACCESSION   DATISU010000228 DATISU010000000
VERSION     DATISU010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710481"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VLR27_09350"
     CDS             complement(<1..261)
                     /locus_tag="VLR27_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HSP03694.1"
                     /translation="MSEPIGLRERKKRDRRRQIEDVAIELFEQQGFDATTIEQIAAAA
                     EIAPRTFFSYFETKDDVVLADYADRLGRILDELDQRPADEPTW"
     gene            342..>2318
                     /locus_tag="VLR27_09355"
     CDS             342..>2318
                     /locus_tag="VLR27_09355"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HSP03695.1"
                     /translation="MSNGLFRLGRWVAVHRRLVLVVWLAVVVGLGVADRVGGGGTVDD
                     FEVPGVESQEAIDLLEDRFPERSGATAMVVFHVPDGGITAGGSTDAIAATVEEVRALD
                     GVVGVTEPLASPRSVSPDGTTAFAAVQFDAPTADLGRSTLDALLDTAEPAEAAGVQVE
                     FGGELPTVLKERHTGPAEMIGIIAALIILFVTFRAVLATVMPLGVAIAGLAVGLSIVG
                     LLGAWIAIPSVAPRLGTMIGLGVGIDYALFVLSRHRDQLAEGMDVTESIGRTNATAGQ
                     AVVVAGGTVVVAILGLQLAGIPFVAALGYSASIVVAVAVLVAITLLPALLGFAGHRVL
                     PRATRSAPPVEGAGHSGWVRWAHWVAHHPWRSAIAATVVLLTLAVPVLDMRLGQADAG
                     TDPTSTTHRRAYDLLAAGFGDGFNGPLLIAVDLGDAPDPTALDPIVAAVAADPGVRAV
                     SPPELNAAGDTGLITAIPTSKPQDGATSDLVHRLRDTVLPAATADTGADALVGGPTAG
                     FIDQSDKIADRLPWFIAAVVSLSFLLLMTVFRSILVPLKAALLNLLAIGAAYGVVVAV
                     FQWGWGRSLIGLDEAVPIASFVPMMMFAILFGLSMDYEVFILSRIREEYHRGHSNIDS
                     VVEGLGATAKVITAAALIMISVFLGFVASDDPVVK"
BASE COUNT          284 a          825 c          811 g          398 t
ORIGIN      
        1 ccacgtcggc tcgtccgccg gtcgctggtc gagctcgtcg aggatgcgac cgagccggtc
       61 ggcgtagtcg gcgaggacca cgtcgtcctt ggtctcgaag tacgagaaga acgtcctcgg
      121 tgcgatctcg gcggcggcgg cgatctgctc gatcgtggtc gcgtcgaacc cctgctgctc
      181 gaacagctcg atggcgacgt cctcgatctg gcggcggcgg tcgcgcttct tgcgctcccg
      241 caagccgatg ggctccgaca tgacgtcatc gtagccatcg aaagatgcga tggttgcaaa
      301 attgcagtga ctgcagttat ggtcggtgtc ggaggtcgat catgtcgaac gggttgttcc
      361 ggttgggtcg ttgggtcgcc gtgcaccggc gcctcgtgct ggtggtgtgg ttggcggtgg
      421 tcgtcggtct gggggtggcg gaccgggtcg gcggcggtgg gacggtcgac gacttcgagg
      481 tccccggcgt cgagtcgcag gaggcgatcg acctgctgga ggaccggttc cccgagcgtt
      541 cgggcgcgac cgcgatggtc gtgttccacg tgcccgatgg cgggatcacc gcgggcgggt
      601 ccacggacgc gatcgccgcg acggtggagg aggtccgagc gctcgacggc gtcgtcggcg
      661 tgaccgagcc gctcgcgagc ccacgatcgg tctcgcccga cggcaccacg gcgttcgccg
      721 ccgtgcagtt cgacgcgccg accgctgacc tcggacggtc caccctcgac gcgctgctcg
      781 acaccgccga gccggccgag gccgccggcg tgcaggtcga gttcggcggc gagctcccga
      841 ccgtgctgaa ggagcgccac accgggccgg ccgagatgat cggaatcatc gccgcgctca
      901 tcatcctctt cgtgacgttc cgggcggtgc ttgccacggt gatgcccctc ggcgtcgcca
      961 tcgccgggct cgcggtgggg ctctcgatcg tggggctgct gggcgcgtgg atcgccatcc
     1021 cctcggtggc gccccggctc gggaccatga tcgggctcgg ggtcggcatc gactacgcgc
     1081 tcttcgtcct cagtcggcac cgcgaccagc tcgccgaggg catggacgtc accgagtcga
     1141 tcggtcggac gaacgccacc gccggccagg cggtggtggt cgccggcgga accgtcgtgg
     1201 tcgccatcct cggcctccag ctcgccggca tccccttcgt ggccgcgctg ggctactcgg
     1261 cctcgatcgt cgtcgcggtg gccgtgctgg tcgcgatcac cctcctgccg gcgctgctcg
     1321 ggttcgccgg ccaccgggtg ctcccgcgag ccacccgcag cgccccaccc gtcgagggtg
     1381 ccgggcacag cggctgggtg cgctgggcgc actgggtggc ccatcacccc tggcgcagcg
     1441 ccatcgccgc aaccgtggtg ctcctgacgc tcgcggtccc ggtcctcgac atgcgactcg
     1501 gccaggccga cgccggcacc gaccccacgt ctacgacgca ccgtcgcgcg tacgacctgc
     1561 tggccgccgg gttcggtgac gggttcaacg ggcccctgct gatcgccgtc gacctcggcg
     1621 atgctcccga cccgaccgcg ctcgatccga tcgtcgccgc ggtggcggcc gatcccgggg
     1681 tccgggcggt ctcacctccg gagctcaacg cggccggcga caccgggctc atcacggcga
     1741 tccccaccag caagccgcag gacggggcca ccagcgatct cgtccaccga ctccgtgaca
     1801 cggtgctgcc cgccgcgacg gccgacaccg gtgcggacgc gctggtgggc ggaccgacgg
     1861 ccggcttcat cgatcagtcc gacaagatcg ccgaccggct gccgtggttc atcgcggccg
     1921 tggtgtcgct gtcgttcctg ttgctgatga ccgtgttccg ctcgatcttg gtcccgctca
     1981 aggccgcgct gctgaacctg ctggccatcg gtgccgccta cggcgtcgtg gtcgccgtgt
     2041 tccagtgggg ctggggccgg tcgctcatcg gcctcgacga ggccgtgccg atcgcctcgt
     2101 tcgtcccgat gatgatgttc gccatcctgt tcggcctgtc catggactac gaggtgttca
     2161 tcctctcccg catccgtgag gagtaccacc ggggccacag caacatcgac agcgtcgtcg
     2221 agggcctcgg tgccaccgcc aaggtcatca ccgccgccgc gctcatcatg atcagcgtct
     2281 tcctcggctt cgtcgccagc gacgatcccg tcgtcaag
//
LOCUS       DATISU010000229         3843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_617590, whole genome shotgun sequence.
ACCESSION   DATISU010000229 DATISU010000000
VERSION     DATISU010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617590"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..719)
                     /gene="udk"
                     /locus_tag="VLR27_09360"
     CDS             complement(48..719)
                     /gene="udk"
                     /locus_tag="VLR27_09360"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF004018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HSP03696.1"
                     /translation="MATTADRSAADDTASGPPPNVVLGVAGGSGSGKTSVVDRIIDEV
                     GAERVAVLRHDRYYHDLRHLPAEDRLAVNVDHPSAFDDELFVDHVRALVRGEAVDSPR
                     YDYATFTRQDGRDRVAPRPVILVEGILLFASASIRELIDVKVYVDADADLRLLRRIRR
                     DVLERGRTVEDVLAQYERTVQPMHLEFVEPSKRWADVIVPRGVENVVGVDLVTSRVES
                     LLARR"
     gene            complement(743..1069)
                     /locus_tag="VLR27_09365"
     CDS             complement(743..1069)
                     /locus_tag="VLR27_09365"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSP03697.1"
                     /translation="MELVSLSDAPAHDPERFVATELLEGTLTNVRVIRLSAGQALPPH
                     THAPSELMLFVVEGVAVLDTDGGDVPFPTGSLARFDGEDELRVSNAGEAPVTLLAFLA
                     PPFPPR"
     gene            complement(1114..1734)
                     /locus_tag="VLR27_09370"
     CDS             complement(1114..1734)
                     /locus_tag="VLR27_09370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HSP03698.1"
                     /translation="MSASGAAGWREVVVEVWTEGQRLSAVGPGDVAVHLAHAERMARL
                     LEAPTRAVDLGSGAGIPGLALAGLWPESEWILLDAAQRRTRLLDAAVERLGWTDRVRV
                     VHGRAEDLGRDPDHRGRYDLVTARLFGPPAAALECGAPLLRTGGVLAVTEPPDPDPDR
                     WPPDALAPLGLDLLEPDPGLQRFRSVRPPEARFPRKPGVPVRRPLF"
     gene            complement(1824..2750)
                     /gene="jag"
                     /locus_tag="VLR27_09375"
     CDS             complement(1824..2750)
                     /gene="jag"
                     /locus_tag="VLR27_09375"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HSP03699.1"
                     /translation="MVTTGRTVDEARERALDALGIDASDLEIEVLDEPGTSLFGLRRT
                     DARIRARVRPTRPRPKLDRRNRRKGGAGGGGRRRRSKKDQDATQKPESEPKSQPAPAP
                     EPAEPANGDAEAPADTPAPPAPARGRGRRRAETTKTDDRKPRTGTTGDTMSDITLDEQ
                     EPSALGFVQGLVDAFGADADVQATSEDDEHLEIAVTGEDLGLLVGPRGATLNAIQELT
                     RTAVAQQHSGRLQGRLRVDVAGYRARRKEALERFAAQQADRVKETGTPVALEPMSPPD
                     RKIVHDAVNEIDGVHTTSEGEDRRRHVVILPD"
     gene            complement(2767..3741)
                     /locus_tag="VLR27_09380"
     CDS             complement(2767..3741)
                     /locus_tag="VLR27_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HSP03700.1"
                     /translation="MFDGIFEFSAQILNFFYGLVPNYAAAIIMLTLLVMLITTPLTLK
                     GTRSMIKMQLLQPELKKIQQKHKGGDRNEMNQELMAFYQENELNPLGGCFPLLVQAPV
                     FLLLFRLIQGLTSRGDDGTFNPKYLDEASDLYQSLDGRTEMISFGLDLSESTTDALGQ
                     SILHGLPYLALILIMIGAQYLQQKQVSARGSSAAAMPQQQMLLKVMPAFFGVISIGFP
                     AALVVYWVTSSIYRIGLQGYITKSLYGGEDSLGAQARKAADEARAMKDQEKAKGSGGA
                     GKAASTKTPAPKSSGPKSTSPTEGGSGNGRTEAGRYQPASKKKKRKKR"
BASE COUNT          524 a         1307 c         1436 g          576 t
ORIGIN      
        1 gacggcagca agccctaccg gctccgatgt ttcacgtgga acatcgctca gcgacgggcc
       61 aggagggact cgacccgaga agtcacgagg tccaccccga cgacgttctc cacgccccgt
      121 gggacgatca cgtcggccca gcgcttcgac ggctccacga actcgaggtg catcggctgc
      181 accgtgcgct cgtactgggc cagcacgtcc tcgacggtgc gaccccgctc caggacgtcg
      241 cgacggatcc ggcggagcag ccgcaggtcg gcgtcggcgt cgacgtagac cttgacgtcg
      301 atgagctcgc ggatgctcgc cgacgcgaac agcaggatcc cctcgaccag gatcacgggc
      361 cggggcgcga cccggtcccg gccgtcctgt cgggtgaagg tggcgtagtc gtagcgaggg
      421 ctgtccaccg cctcgccccg gaccagggcc cgcacgtggt cgacgaacag ctcgtcgtcg
      481 aaggcggagg ggtggtcgac gttgaccgcg aggcggtcct cggccggcag gtgccgcaga
      541 tcgtggtagt agcggtcgtg gcgcagcacg gcgacccgct cggcaccgac ctcgtcgatg
      601 atgcggtcga cgacgctggt cttgcccgac cccgagccac cggcgacccc gagcacgacg
      661 ttcggtgggg gaccgctggc ggtgtcgtcg gcggccgagc ggtcagcggt ggttgccacg
      721 ccggccattg caccacatgg cctcagcggg gcgggaaggg cggagccagg aaggcgagca
      781 gggtcaccgg cgcctcaccg gcgttggaca cccgcagctc gtcctcgcca tcgaagcggg
      841 ccagcgagcc ggtggggaac gggacgtccc cgccgtcggt gtccagcacg gcaacgccct
      901 cgaccacgaa cagcatcagc tccgacgggg cgtgcgtgtg gggcggcagc gcctggccgg
      961 cgctcagccg gatgacccgg acgttcgtca gggtcccctc cagcagctcg gtggcgacga
     1021 agcgctccgg gtcgtgggcg ggagcatcgg acagggagac gagttccatg gaccgagtat
     1081 gggggcaggg tctgtacctg cggtgccgtg gcctcagaag agcggccgac ggaccggaac
     1141 gcccggcttg cgcgggaagc gcgcctcagg cgggcggacc gaacggaacc gctggagccc
     1201 agggtcgggt tcgagcaggt cgaggccgag cggggccagg gcatcgggtg gccagcggtc
     1261 cgggtccggg tccgggggtt cggtgaccgc gagaacccca ccggtgcgca gcaggggagc
     1321 gccgcactcg agcgccgcgg caggtggacc gaacagccgg gcagtcacca ggtcgtagcg
     1381 cccgcgatga tcgggatcgc gaccgaggtc ctcggcccgg ccgtggacga cccgcacccg
     1441 atcggtccaa ccgaggcgct cgacggctgc gtccaggagg cgggtccgac gctgcgccgc
     1501 gtcgagcagg atccactcgc tctccggcca caggccggcg agcgccagac ccgggatgcc
     1561 ggcgccgctc cccaggtcga ccgccctcgt cggcgcctcg agcaaccgag ccatgcgctc
     1621 ggcgtgagcc aggtggacgg cgacgtctcc gggccccacg gcggagagcc gttgcccctc
     1681 ggtccagacc tcgaccacga cctcacgcca gcccgcggcg cccgaagcgc tcacgcgacg
     1741 tgcccctggt cggtgcccgg gaaggccact ggccgaccac ccgggtcggg cagtcggcca
     1801 gcgagtgcga acatccgttc gtgtcagtcg gggaggatga cgacgtggcg ccggcgatcc
     1861 tcgccctcgc tcgtcgtgtg cacgccgtcg atctcgttga cggcgtcgtg cacgatcttg
     1921 cggtccgggg gcgacatcgg ctcgagggcg accggcgtgc cggtctcctt gacccggtcg
     1981 gcctgctggg cggcgaagcg ctcgagcgcc tccttgcgac gggcccggta gccggcgacg
     2041 tcgacccgca ggcgaccctg cagccgcccc gagtgctgct gggccacggc cgtgcgggtc
     2101 agctcctgga tcgcgttcag ggtggcacca cgaggcccga ccaggagccc gaggtcctca
     2161 ccggtgacgg cgatctccag gtgctcgtcg tcctcgctgg tggcctggac gtcggcgtcg
     2221 gcgccgaagg cgtccacgag gccctgcacg aacccgaggg cgctcggctc ctgctcgtcg
     2281 agggtgatgt cgctcatggt gtctcctgtc gtccccgtgc ggggcttgcg gtcatcggtc
     2341 ttggtggtct cggcgcgccg gcggccccgg cctcgagcag gggcgggggg cgccggggtg
     2401 tctgccggag cctcagcatc gccgttcgcg ggctctgccg gctcgggcgc tggtgccggc
     2461 tgcgacttcg gctccgactc gggcttctgg gtggcgtcct ggtccttctt ggaccgccgg
     2521 cgacgtccgc ccccgccggc gccacccttg cggcggttgc gacggtcgag cttcgggcgg
     2581 ggacgcgtcg ggcggacgcg ggcgcggatt cgggcgtcgg tccggcggag gccgaacagc
     2641 gacgtcccgg gctcgtcgag cacctcgatc tcgaggtcgc tggcgtcgat cccgagggca
     2701 tcgagggctc gttcgcgcgc ctcgtccacg gtccggccag tggtgaccac ccactgcacc
     2761 tgcccgctac ctcttcttcc gcttcttctt cttggacgcc ggctggtacc ggccggcctc
     2821 ggtgcgcccg ttgccggacc cgccttcggt ggggctcgtc gacttcgggc ccgaggactt
     2881 gggcgcaggg gtcttggtgg acgcggcctt gccggcgccg ccggagccct tggccttctc
     2941 ctggtccttc atcgcccggg cctcgtcggc tgccttgcgg gcctgggcac cgagggagtc
     3001 ctcaccgccg tagagggact tggtgatgta gccctgcagg ccgatgcggt agatgctcga
     3061 ggtcacccag tacacgacga gcgcggccgg gaagccgatc gagatgacgc cgaagaacgc
     3121 cggcatgacc ttgagcagca tctgctgctg gggcatcgcc gccgcgctgc tgcctcgggc
     3181 cgacacctgc ttctgctgga ggtactgggc gccgatcatg atcaggatga gcgccaggta
     3241 gggcaggcca tggaggatcg actgcccgag ggcgtcggtg gtcgactccg acaggtcgag
     3301 accgaagctg atcatctcgg tccggccgtc gagggactgg tagaggtcgc tggcctcgtc
     3361 gaggtacttc gggttgaagg tgccgtcatc cccccggctg gtgaggccct ggatgaggcg
     3421 gaacagcagg aggaagacgg gggcctggac caggagcggg aagcacccgc cgagcgggtt
     3481 cagctcgttc tcctggtaga acgccatcag ctcctggttc atctcgttgc ggtcgccgcc
     3541 cttgtgcttc tgctggatct tcttcagctc cggctgcagc agctgcatct tgatcatcga
     3601 gcgcgtgccc ttgagggtga gcggcgtcgt gatcagcatc accagcagcg tgagcatgat
     3661 gatggcggcg gcgtagttgg gaaccaggcc gtagaagaag ttcaggatct gggcgctgaa
     3721 ctcgaagatt ccgtcgaaca tctcggcgtc ctatcggggg tggtgggagg cgggctcggg
     3781 cacgcccggc acggggtcgt agccgtggcc gccccagggg gcgcagcgga cgatgcggcg
     3841 cac
//
LOCUS       DATISU010000230         6054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_617770, whole genome shotgun sequence.
ACCESSION   DATISU010000230 DATISU010000000
VERSION     DATISU010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617770"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VLR27_09385"
     CDS             complement(<1..632)
                     /locus_tag="VLR27_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSP03701.1"
                     /translation="MSDQGPVVYGGRYELHRRLARGGMADVYLARDQLLDRPVAVKVL
                     FPQYAADPSFVQRFRREAQDSANLNHPNIVGVYDWGEEGGTYFIVMEYVAGRSLSDVL
                     RQEGSLLPARAADIGIDMAAALGFAHKNGVVHRDVKPGNVLLSTDGQVKVTDFGIARA
                     ISSGHEEDLTQAGQVMGTATYFSPEQAQGRPVDPRSDVYSLGVVLYEMVCG"
     gene            complement(672..2150)
                     /locus_tag="VLR27_09390"
     CDS             complement(672..2150)
                     /locus_tag="VLR27_09390"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HSP03702.1"
                     /translation="MSNQIAKLGVGLLLCFLVLFVQLNRVTVFGAQELKDNPVNNRSI
                     LRDFDAPRGTIVTADEVIVARSEPTPEGARFEFARTYPEGDLFAHTVGYFSLNLGATG
                     VEDTYNAELAGRTLDLSFRSLDDLFVDRDRVGDVRLTLRADAQRIAKGELGERQGSVV
                     AIDPRSGELLAMWSWPSFDPNAIATHDFGAANDAFSLFQSDERDPLLAKTYRERFFPG
                     STFKIVTAAAGLASGAVTADQPAYPVAREYTPPQTNRPLRNFGGSSCGGNLFDILRVS
                     CNTAFAQMAIDLGEGQMVDTAESFGFNAAPPIDLPAPAQSAFPTDFDQNLPALAQAGI
                     GQNDVSATPLQMAMVAAAVANEGRIMTPRVVADIRDDDNTVVDDPEATQWRQALSAED
                     ADVLRRAMVEVAERGTARNLQIPGFEVGGKTGTAQLGTDPPRSHAWIVGFAGPPGQPP
                     SVAVAVIVEGQPGASEQTGGQVAAPIARAVMETVLAAQGGGA"
     gene            complement(2147..3460)
                     /locus_tag="VLR27_09395"
     CDS             complement(2147..3460)
                     /locus_tag="VLR27_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HSP03703.1"
                     /translation="MILLRRRTELGLIVVGALITAGAYALAGLGRDAEIPANLVPFLG
                     IVLGLLVTAHLAVRRLAPDADGILLPLAALLNGIGYVFIVRLDEAQADGGRLAGLQAM
                     WTAVGIGAFIATLLFVRRSRSLERYRYTFAAIGLGLLLLPLLPVIGRTINGSRIWISI
                     GPANFQPGEAAKIVLALFFAGYLVERRELLALSTFRLGPLHLPDPKHLGPILLAWGVS
                     LGVMTLQKDLGSSLLFFVLFVVMLWIATGRVTYLTLGGTLFLAGATFAYTQFSHVQDR
                     VAVWLDPWPVADDEGYQIVEASFALADGGIAGTGIALGTPTRIPEVETDFIFAAIGEE
                     LGLIGATAVLCAYLLMVGAGLRVALRADSPFDTLLASGLTTLLGVQTFIIIGGITRTL
                     PLTGVTLPFVSYGGSSLVMNYVLLALLLRISDNSVAARTPARAGA"
     gene            complement(3457..4815)
                     /locus_tag="VLR27_09400"
     CDS             complement(3457..4815)
                     /locus_tag="VLR27_09400"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HSP03704.1"
                     /translation="MSDPGGNTASGVRLRWGGASDVGQVRQVNQDSLYVGTGLFVVAD
                     GMGGHAGGEIASRLAVETMESELAHEGSGPWRVLDLVTAVQAANDRVWAQSEHDPNLA
                     GMGTTVVAIGIVEEDGEARVAIVNVGDSRAYRLSDGHLDQVSDDHSLVGELFREGRLT
                     VDEARVHPQKNIVTRAVGIERYVDVDEFQILPHTGDRYLLCSDGLTDEVTEDEITQVL
                     RSIDDPEAAAQELVRQANAAGGRDNITLIVVDVVDDGRGPTGASDARRESVPDDDDFS
                     HDPDQDTQSLLAVPDDQAHLQRAGAPTDEGDRRADEPRAKAPRAVTVRSLAFVTVVLA
                     LVGVGAFLAYRYVQDTYHVGLEDDRVVIFEGRPGGFLWFDPSPVEDTGIDVDDVPAAV
                     LDDLEAGVSQPTLQRARRYVTNLEDRIEDTRPPVTTTTTTTTTTTTTTAPSPTTTGVA
                     GG"
     gene            complement(4812..5312)
                     /locus_tag="VLR27_09405"
     CDS             complement(4812..5312)
                     /locus_tag="VLR27_09405"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HSP03705.1"
                     /translation="MPEPLLNVLKICLLILLYLFFFRVLRAVWAEVHLARQTSSGTTS
                     GRSGRGGRSGKAGGAGRAPRRTRARERTPPRLKVIEPAELAGTTYPLGDELTVGRAAG
                     CQITLDDTYASQVHARVFMRDDQLYVEDLGSTNGTYLNRRKVSAPMVVSHGDQIQIGS
                     TIMEVS"
     gene            complement(5315..5974)
                     /locus_tag="VLR27_09410"
     CDS             complement(5315..5974)
                     /locus_tag="VLR27_09410"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HSP03706.1"
                     /translation="MVLRNFERRLERIVEGVFSRAFKSGLRPIEIGRRLTREMDDTRS
                     VGVRGGTVVANHFTVNLSQQDADEFAEVTDTMQRELAELAREHAREEHYTFMGPVEIL
                     FHVDPKMRVGAFSVEARLREGDGGAGAGAIVLPSGERFVLGDTIVTIGRLPESVLVLE
                     DPNVSRQHAEIRPSGNGFVLADLGSTNGSKVNGIKVSERVLQDGDELTFGSTSFRFEA
                     S"
BASE COUNT          871 a         2024 c         2314 g          845 t
ORIGIN      
        1 ccgcacacca tctcgtagag caccacgccg aggctgtaga cgtcggagcg gggatcgacc
       61 ggccggccct gggcctgctc gggcgagaag taggtggccg tgcccatgac ctgaccggcc
      121 tgggtgaggt cctcctcgtg cccggagctg atggcccggg cgatgccgaa gtcggtgacc
      181 ttgacctgcc cgtcggtgga cagcagcacg ttgcccggct tgacgtcgcg gtggacgacg
      241 ccgttcttgt gggcgaagcc gagcgcggcg gccatgtcga tgccgatgtc ggcggcccga
      301 gccggcagca gcgagccctc ctggcgcagc acgtcggaca ggctccggcc ggccacgtac
      361 tccatcacga tgaagtaggt gccgccctcc tcgccccagt cgtacacgcc cacgatgttg
      421 gggtggttga ggttcgccga gtcctgcgcc tcccgccgga accgctgcac gaagctggga
      481 tctgcggcgt actgggggaa caggaccttg accgcgacgg ggcggtcgag gagctggtct
      541 cgggccaggt agacgtcggc catgccgccc cgagcgaggc ggcggtggag ctcgtagcgg
      601 cccccgtaga cgactggtcc ctggtcagac atggcgcggc gcactctacg acaggggccc
      661 ccgccggttg gctacgcgcc accaccctgc gcggccagca ccgtctccat gaccgcccgg
      721 gcgatcggcg ccgccacctg cccgccggtc tgctcggacg cgccgggctg gccctcgacg
      781 atgaccgcaa cggcgaccga cggcggctgc ccggggggac cggcgaagcc gacgatccag
      841 gcatgggaac ggggcgggtc cgtgccgagc tgggccgtgc cggtcttgcc gcccacctcg
      901 aagccgggga tctgcaggtt gcgcgccgtg ccccgctcgg ccacctcgac catggcgcgg
      961 cgcagcacat cggcgtcctc cgccgagagg gcctggcgcc actgggtggc ctcggggtcg
     1021 tcgaccacgg tgttgtcgtc gtctcggatg tcggccacca cccggggcgt catgatccgg
     1081 ccctcgttgg cgaccgccgc ggcgaccatg gccatctgca gcggcgtggc ggacacgtcg
     1141 ttctggccga tgccggcctg ggccagcgcc ggcaggttct ggtcgaagtc cgtcgggaac
     1201 gccgactggg ccggggcggg caggtcgatg ggtggggcgg cgttgaagcc gaaggactcg
     1261 gccgtgtcca ccatctggcc ctcgccgagg tcgatggcca tctgggcgaa ggcggtgttg
     1321 cacgacaccc gcaggatgtc gaagaggttc ccgccgcacg acgagccgcc gaagttccgc
     1381 agcggccggt tggtctgggg tggggtgtac tcccgggcga cggggtacgc cggctggtct
     1441 gcggtgaccg cgcccgaggc caggccggcg gcggcggtga cgatcttgaa cgtcgagccg
     1501 gggaagaagc gctcgcggta ggtcttggcc agcagcgggt cgcgctcgtc ggactggaac
     1561 agggagaacg cgtcgttggc ggccccgaag tcgtgggtgg cgatggcgtt ggggtcgaac
     1621 gacggccacg accacatggc gagcagctca ccggagcgcg ggtcgatggc cacgaccgag
     1681 ccctggcgct cgcccagctc gcccttggcg atgcgctggg cgtccgcccg cagggtgagg
     1741 cgcacgtcgc cgacgcggtc ccgatcgacg aagaggtcgt cgaggctgcg gaacgacagg
     1801 tcgagggtgc gcccggccag ctcggcgttg taggtgtcct ccacgcccgt ggccccgagg
     1861 ttcagcgaga agtagccgac cgtgtgggcg aacaggtcgc cctcggggta cgtgcgggcg
     1921 aactcgaagc gggcgccctc gggcgtcggt tcggaccggg cgacgatgac ctcgtcggcg
     1981 gtgacgatgg tgccccgcgg cgcatcgaag tcccgcagga tcgaccggtt gttcaccggg
     2041 ttgtccttca gctcctgcgc gccgaagacc gtgacccggt tgagctgcac gaacagcacg
     2101 aggaagcaca gcaggaggcc gacgccgagc ttggcgatct ggttgctcat gcgcccgccc
     2161 tggccggggt gcgggccgcc acgctgttgt ccgagatccg cagcaggagg gcgagcagca
     2221 cgtagttcat gacgaggctg gagccgccgt agctgacgaa gggcagcgtg acgccggtga
     2281 ggggcagcgt ccgggtgatg ccgccgatga tgatgaaggt ctggacgccg aggagcgtgg
     2341 tgaggccgct ggcgagcagg gtgtcgaacg gcgagtcggc ccgcagggcg acccggaggc
     2401 cggcgccgac catcagcagg taggcgcaca gcacggcggt ggcgccgatc agcccgagct
     2461 cctcgccgat ggcggcgaag atgaagtcgg tctcgacctc ggggatgcgg gtcggcgtgc
     2521 cgagggcgat gccggtgccg gcgatgccgc cgtcggccag ggcgaaggac gcctcgacga
     2581 tctggtagcc ctcgtcgtca gcgaccggcc acggatcgag ccagaccgcg acgcggtcct
     2641 gcacgtggga gaactgggtg taggcgaagg tggcgccggc gaggaagagc gtgccgccga
     2701 gggtgaggta ggtgacccgc ccggtggcga tccacagcat gaccacgaac agcacgaaga
     2761 acaggagcga ggagcccagg tccttctgca gggtcatgac gccgagggac acgccccagg
     2821 cgagcaggat cgggcccagg tgcttcgggt cgggcaggtg gagcggaccg agccggaacg
     2881 tcgacagcgc cagcagctcg cgccgctcga ccaggtagcc ggcgaagaag agggccagca
     2941 cgatcttggc cgcctcaccg ggctggaagt tcgccggtcc gatcgagatc cagatgcggc
     3001 tgccgttgat cgtgcgaccg atgaccggca gcagcggcag caggagcagc ccgaggccga
     3061 tggcggcgaa ggtgtagcgg taccgctcga ggctgcggct gcgccggacg aacaggaggg
     3121 tggcgatgaa cgcgccgatg ccgacggcgg tccacatcgc ctggagcccg gccagacggc
     3181 cgccatcggc ctgggcctcg tcgaggcgga cgatgaagac gtagccgatg ccgttcagca
     3241 gggcggcgag cggcagcagg atgccgtcgg cgtcgggggc gaggcgacgg accgccaggt
     3301 gggcggtgac gagcaggccg agcacgatgc cgaggaacgg caccaggttc gccgggatct
     3361 cggcgtcgcg gccgaggccg gccagcgcgt aggcgccggc cgtgatgagg gcgcccacga
     3421 cgatcaagcc gagctcggtg cgtcggcgga gcaggatcac cccccggcca ccccggtggt
     3481 cgtggggctc ggcgcggtcg tcgtcgtcgt ggtcgtggtc gtggtcgtcg tggtggtgac
     3541 cggcgggcgg gtgtcctcga tgcggtcctc gaggttggtc acgtagcggc gggcccgctg
     3601 cagggtcggc tggctcaccc cggcctcgag gtcgtcgagc acggcggcgg gcacgtcgtc
     3661 gacgtcgatg ccggtgtcct ccaccgggct cgggtcgaac cacaggaacc cgccgggccg
     3721 cccctcgaag atgacgacgc ggtcgtcctc gaggccgacg tggtacgtgt cctgcacgta
     3781 gcggtaggcg aggaacgcgc cgacgccgac cagcgcgagc accacggtga cgaaggccag
     3841 cgagcgcacc gtgacggcgc ggggcgcctt cgcccggggt tcgtccgctc ggcggtcgcc
     3901 ctcgtcggtc ggggcgccgg cccgctggag gtgggcctga tcgtcgggca cggccagcag
     3961 cgactgggtg tcctggtcgg ggtcgtggga gaagtcgtcg tcgtccggga ccgactcgcg
     4021 ccgggcgtcg gacgcgccgg tgggtccccg gccgtcgtcg acgacgtcga ccacgatgag
     4081 ggtgatgttg tcccgcccgc cggcggcgtt ggcctggcgc acgagctcct gggcggcggc
     4141 ttcgggatcg tcgatgctcc gcaggacctg ggtgatctcg tcctcggtca cctcgtcggt
     4201 gaggccgtcg gagcacagca ggtagcggtc gccggtgtgg gggaggatct ggaactcgtc
     4261 gacgtcgacg tagcgctcga tgcccacggc tcgggtgacg atgttcttct gcgggtggac
     4321 ccgggcctcg tcgaccgtga ggcgaccctc ccggaacagc tcgccgacca ggctgtggtc
     4381 gtcgctcacc tggtcgaggt gcccgtcgga gaggcggtac gcccggctgt cgccgacgtt
     4441 gacgatggcc acccgggcct cgccgtcctc ctcgacgatg ccgatggcca cgaccgtggt
     4501 gcccatgccg gccaggttcg ggtcgtgctc gctctgggcc cacacccggt cgttggcggc
     4561 ctggaccgcg gtgaccaggt cgagcacgcg ccagggcccc gacccctcgt gcgcgagctc
     4621 ggactccatc gtctccacgg cgaggcgcga ggcgatctca ccgccggcgt ggccgcccat
     4681 gccgtcggcg acgacgaaga ggccggtgcc gacgtacagc gagtcctggt tgacctggcg
     4741 gacctggccg acgtcggacg caccacccca gcgcaggcgc acgccggatg ccgtgttgcc
     4801 acccggatcg ctcacgagac ctccatgatc gtggagccga tctggatctg gtcgccgtgg
     4861 ctcaccacca tcggcgccga gaccttccgc cggttgaggt aggtgccgtt ggtggagccc
     4921 aggtcctcga cgtagagctg gtcgtcccgc atgaacacgc gcgcgtgcac ctgggacgcg
     4981 taggtgtcgt cgagggtgat ctggcacccg gcggcccggc cgaccgtgag ctcgtcaccc
     5041 agcgggtacg tggtgccggc gagctcggcc ggctcgatga ccttgagccg cggcggcgtg
     5101 cgctcgcggg cccgggtccg tcggggggca cgaccggcgc cgcctgcctt gccgctgcga
     5161 ccaccccgac ccgagcgtcc cgaggtggtg cccgaggacg tctgacgggc gaggtggacc
     5221 tcggcccaga cggcgcgcag cacccggaag aagaacaggt agagcaggat cagcaggcag
     5281 atcttgagga cgttgagcag cggctcgggc acggtcagct ggcctcgaac cggaacgacg
     5341 tcgagccgaa ggtcagctcg tcaccgtcct gcaggacccg ctcgctcacc ttgatgccgt
     5401 tcaccttgga cccgttggtg gatccgaggt cggccagcac gaacccgttg cccgacgggc
     5461 ggatctcggc gtgctggcga gagacgttgg ggtcctcgag caccaggacg ctctcgggca
     5521 gccggccgat ggtgacgatg gtgtcgccga gcacgaagcg ctcacccgag ggcagcacga
     5581 tggcaccggc cccggcgccc ccgtcgccct cccgcaggcg ggcctccacc gagaacgcgc
     5641 ccacccgcat cttggggtcg acgtggaaca ggatctccac cggacccatg aaggtgtagt
     5701 gctcctcgcg ggcgtgctcg cgggccagct cggcgagctc gcgctgcatg gtgtcggtga
     5761 cctcggcgaa ctcgtctgca tcctgctggg acaggttcac cgtgaagtgg ttggcgacga
     5821 ccgtgccgcc acgcaccccg accgagcggg tgtcgtccat ctcgcgggtg aggcgccggc
     5881 cgatctcgat cgggcgcagc ccggacttga acgcccggga gaacacgccc tccacgatgc
     5941 gctcgaggcg ccgttcgaag ttccgcaaga ccatcgggcg ggaggctacc tgcaccaccg
     6001 gcccggctga ccggttcgtc ccgtcatgtc ggctggggcc ccggcccgag gtcg
//
LOCUS       DATISU010000231         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_478365, whole genome shotgun sequence.
ACCESSION   DATISU010000231 DATISU010000000
VERSION     DATISU010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478365"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..307)
                     /locus_tag="VLR27_09415"
     CDS             complement(5..307)
                     /locus_tag="VLR27_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03707.1"
                     /translation="MAEVPDSEYAPSALPPTGARILAFVAILVAGLCGGLIGYAFVDM
                     QTDGDSTVWAGVGGLVGAVIFAVGVGIVAVLAMRAMGEWRVIEHSREQADRRGPIS"
     gene            414..1376
                     /gene="coaA"
                     /locus_tag="VLR27_09420"
     CDS             414..1376
                     /gene="coaA"
                     /locus_tag="VLR27_09420"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HSP03708.1"
                     /translation="MTEQQAGPAGAERWGERRFERAEWAALRAHESVPIHEEDLEELE
                     GINEHLSMGEVEDVYVPLARLLHLRAGVTRSLHDATGAFLGRFRPHVPFVIGVAGSVA
                     VGKSTTSRVLQTLLARAPERRRVELVTTDGFLLPNAELEDRGLMQRKGFPESYDTPRL
                     VRFLADLKAGRPALEIPVYSHVIYDVVDEVQVVDQPDIVIVEGLNVLQVPSTTGEHHP
                     SVFVSDFFDFSIYVDADADHIEEWYLQRFMTLVEGSRHDDGAFFHRFAGLDDESANAL
                     ARSIWRAVNLPNLHDNVLPTRDRADLVLHKDADHEVRQVVLRLR"
     gene            1403..2134
                     /locus_tag="VLR27_09425"
     CDS             1403..2134
                     /locus_tag="VLR27_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03709.1"
                     /translation="MSEHAFRPLGAATPVVEATAGAAAAVVMGAASALRRKRVFHPDG
                     VAFAGNVQVTGAHHGAPLLDEAGKYDAVVRLSRGVGLPRAFPDFLGLALRITDAHGPG
                     DHQDLLLVSAGDRPVLRHTLVPVRGFDHRRFSTLLPYEVGERRVVFGARVLGEGGPVP
                     LQLEDLRDRAANTGLRFALELAEASGDWEPIGVIDVGAPLTDDDGDALRFDPAHTGGG
                     IEPVGVVQTVRRLAYRASQTGRPTT"
BASE COUNT          289 a          823 c          724 g          298 t
ORIGIN      
        1 acgctcagct gatcggtccg cggcggtcgg cctgctcccg ggagtgctcg atcacccgcc
       61 actcgcccat ggcccgcatg gccaggacgg ccacgatgcc cacgccgacg gcgaagatca
      121 cggcgccgac caacccgccg acgcccgccc acaccgtgct gtcgccgtcg gtctgcatgt
      181 cgacgaaggc gtagccgatg agcccgccgc acaggccggc caccaggatc gccacgaagg
      241 cgaggatccg ggcgcccgtc ggcgggagcg cggagggggc gtactcggag tcggggacct
      301 cggccacgcc gcgaggctag agccgatccc gacatctcgt ggattcgcca ccacctgcgg
      361 caccgatcca cgggatttcc ggcgggtggg ggtcgtgcct aggttggccg accgtgaccg
      421 agcagcaggc gggacccgcc ggggcggagc ggtggggcga gcgccgcttc gagcgggccg
      481 agtgggccgc gctgcgggcg cacgagtcgg tccccatcca cgaggaggac ctcgaggagc
      541 tcgagggcat caacgagcac ctgtcgatgg gcgaggtcga ggacgtctac gtgcccctcg
      601 cccgcctcct gcacctgcgc gccggcgtca cccgctccct gcacgacgcc accggcgcct
      661 tcctcgggcg gttccgcccc cacgtcccct tcgtcatcgg cgtcgccgga tcggtggcgg
      721 tgggcaagtc gaccacctcg cgggtgctcc agaccctgct ggcccgggcc ccggagcgcc
      781 gccgggtcga gctcgtcacc accgacgggt tcctgctgcc caacgccgag ctcgaggacc
      841 gtggcctcat gcagcgcaag ggcttccccg agagctacga caccccgcgc ctggtccgct
      901 tcctggccga cctcaaggcg ggtcggcccg cgctcgagat cccggtgtac agccacgtca
      961 tctacgacgt cgtcgacgag gtccaggtgg tcgaccagcc cgacatcgtg atcgtcgagg
     1021 ggctcaacgt gctgcaggtc ccttccacca cgggtgagca ccacccgtcg gtcttcgtgt
     1081 cggacttctt cgacttctcg atctacgtcg acgcggacgc cgaccacatc gaggagtggt
     1141 acctgcagcg gttcatgacg ctggtcgagg gctcccgcca cgacgacggg gcgttcttcc
     1201 accgcttcgc cggcctcgac gacgagtcgg cgaacgccct ggcccgcagc atctggcgcg
     1261 ccgtgaacct gccgaacctg cacgacaacg tgctgcccac ccgggaccgg gccgacctgg
     1321 tgctgcacaa ggacgccgac cacgaggtcc gccaggtggt gctccgactt cgctgagtgg
     1381 ctcgtctggg tagggacgga ccatgtctga acacgccttc cgtcccctcg gtgctgccac
     1441 cccggtcgtc gaggccaccg ccggcgccgc tgctgcggtc gtcatgggcg ccgcttcggc
     1501 cctgcgccgc aagcgggtgt tccaccccga cggcgtcgcg ttcgcaggca acgtccaggt
     1561 cacgggcgcc caccacggcg cacccctcct cgacgaggcg ggcaagtacg acgccgtcgt
     1621 gcggctgtcc cgcggcgttg gcctgccccg ggcgttcccc gacttcctcg gcctcgccct
     1681 gcgcatcacc gacgcccacg gacctggcga ccaccaggac ctcctcctgg tcagcgccgg
     1741 cgaccgaccc gtgctccgcc acacgctggt gcccgtgcgg ggcttcgacc accgccggtt
     1801 ctccacgctc ctgccctacg aggtgggcga gcgcagggtc gtcttcggcg cccgggtgct
     1861 gggggagggc ggacccgtcc cgctgcagct cgaggacctc cgcgaccggg ccgccaacac
     1921 cggcctgcgc ttcgccctcg agctggccga ggcgtcgggg gactgggagc cgatcggggt
     1981 catcgacgtc ggcgcacccc tcacggacga cgacggggac gccctccgct tcgacccggc
     2041 gcacaccggt ggcggcatcg agcccgtcgg cgtggtccag accgtgcggc gtctcgccta
     2101 tcgcgccagc cagacgggtc gacccaccac ctga
//
LOCUS       DATISU010000232         5929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_710800, whole genome shotgun sequence.
ACCESSION   DATISU010000232 DATISU010000000
VERSION     DATISU010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710800"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /gene="mihF"
                     /locus_tag="VLR27_09430"
     CDS             <1..298
                     /gene="mihF"
                     /locus_tag="VLR27_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HSP03710.1"
                     /translation="TPEQRQAALAKAAEARRARAELKERLKMGSLLLSELLEQAPGNE
                     TIGKMKVLAVLESLPGVGKVKARRTMEAFEISETRRVRGLGEQQKRKLLEEFGS"
     gene            336..872
                     /locus_tag="VLR27_09435"
     CDS             336..872
                     /locus_tag="VLR27_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03711.1"
                     /translation="MNIVVSGPGGVGKGTIVDQLLARRPEIWLSRSWTTRERRAGEAE
                     DAYVFTDRESFDAHIDAGGFLEWVDFLDYRQGTPVPNPPPGTDVLLEIDVAGAEVIRA
                     VDPDALLVFVDTPDREAQRQRLTGRGDSPDRIEARMRKGDEERVAARRLGMHHIVNDV
                     LHDAVAELEALIDAHRRR"
     gene            937..1260
                     /gene="rpoZ"
                     /locus_tag="VLR27_09440"
     CDS             937..1260
                     /gene="rpoZ"
                     /locus_tag="VLR27_09440"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HSP03712.1"
                     /translation="MSGRHDTMMNPRIEDLLDRTDSKFRLVTLGSTRARQINSYFNAV
                     GSGGLGSQVPPQVPSVVRKPLSIAFEEIAADKILPVYGEELAAKREAEAAEAAARIAA
                     AEDQG"
     gene            1284..2513
                     /gene="coaBC"
                     /locus_tag="VLR27_09445"
     CDS             1284..2513
                     /gene="coaBC"
                     /locus_tag="VLR27_09445"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HSP03713.1"
                     /translation="MLAGKHIVLGVTGGIAAYKAVEVCRRLVDAGAHVTPIMTEGARH
                     FVGETTLSTLASETVRTSLWEIDHGADGSPITHTRLGQTADLVIVAPATARVISDLRT
                     GRSADLLSATLIATTAPVLLAPAMHTEMWEHPAVRENLEVLATRGVHVVPPEEGRLAG
                     GDSGAGRLADPATIVEAAEKVLAGSGADADLAGLAVLVTAGGTREPIDPVRFIGNRSS
                     GKQGHAIAEEAAARGARVTLVTTVDRPASAGVEIVRVDTADEMHAAVAERASSSDVVV
                     MAAAVADFKPAAPADRKIKKAGGTPEVVLEPTVDILASLGASKPAGQTLVGFAAETDD
                     LRANAQAKLAAKGADLIVANDVSAPGAGFEHDTNEVVLLSAGGATEVPQADKRQIARA
                     VLDAVITLRSPNPGSQT"
     gene            2510..3709
                     /gene="metK"
                     /locus_tag="VLR27_09450"
     CDS             2510..3709
                     /gene="metK"
                     /locus_tag="VLR27_09450"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HSP03714.1"
                     /translation="MKNDFTFTSESVSEGHPDKMADQISDAILDAILAEDPMARVACE
                     TMVTTGMAIVSGEITTTAYVEIPDIVRKTINGIGYDRESMGFDGNTCGVMTTIDPQSP
                     DIAQGVDKAFETRTGERDDELDDQGAGDQGMMFGYACDENDAMMPTPIHLAHRLAERL
                     AEVRKAGILPYLRPDSKTQVTIDYRDGRPAALDTVLISTQHAPGHDADTLIKPDLIEH
                     VIRPTLPEAFADDDFRVLVNPTGKFELGGPHADCGLTGRKIIVDTYGGWARHGGGAFS
                     GKDPSKVDRSAAYATRWVAKNLVAAGAASRCEVQVAYAIGVAHPMSIMVETFGTENVD
                     PARIEEAVREVFDLRPGAILRDLDLRRPIYRKTAAYGHFGRTGVDGEFPWENTNRVDD
                     VKSALGL"
     gene            3809..5548
                     /locus_tag="VLR27_09455"
     CDS             3809..5548
                     /locus_tag="VLR27_09455"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03715.1"
                     /translation="MRVLPDQTGTAKTFDYLVPEAWADGVRVQVGSRVRIALGPRRVG
                     AWVTDVDVDPPAGVVLKPIAKLSGHGPSAELIDLARWAQWRWAAKTPVPFLRTASPPR
                     NVAGLPAGPARTQPLPQVADPVVAPLLDDALAGGPVVLRLPPTADLAAVALGAARAGD
                     ALVICPSHRMARHLAVRLRRAGVAVALHPDEWARAAAGGCTVIGTRASAWAPVPDLAA
                     VVVLDEHDEVHQEERSPTWHARDVALERARRRGVPCVLTSPMPTLEALPVARLIRTDR
                     ATERSGWPAAVVADRTEEPPGRNALFSPQLVDVIRSGARVLCVLNQKGRAALLGCVGC
                     GEIVRCDACQAAVAKPGDELVCRRCGTTRPVICATCGSIDLKVIRMGVNRVVEDLAAL
                     SGERVVAVTAETPAADVDSARLYVGTEAVLHQVGRADVVAFLDLDQELFAPRYRAAEE
                     ALGLVVRAARIVGGRGEGGRVLLQTRSPEHEVCRAALLGDPERVASAEDERRAMLGFP
                     PHVALAEVGDQAGEAFVAALTGSAPPDVQVQGPVDGAWRVRAPDHQTLCDALAAVHRP
                     PGRLRLSVDPLRI"
     gene            complement(5545..>5929)
                     /locus_tag="VLR27_09460"
     CDS             complement(5545..>5929)
                     /locus_tag="VLR27_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSP03716.1"
                     /translation="IAAVLARTSPDARVVAVDVNERSRELCARNATDLGCTNVEVLAP
                     DDVDPALRFVLIWSNPPIRIGKDALRELLLRWLDLLTPDGEAVLVMGRNLGADSMQRW
                     LGEQGFPTERLASKRGYRLLRSQRA"
BASE COUNT          818 a         2316 c         2054 g          741 t
ORIGIN      
        1 caccccggag cagcgccagg cggcgctggc caaggccgcc gaggcccgcc gggcccgagc
       61 cgagctgaag gaacgcctga agatgggctc gctcctgctg tccgagctgc tcgagcaggc
      121 gccgggcaac gagacgatcg gcaagatgaa ggtcctggcc gtgctcgagt ccctccccgg
      181 tgtggggaag gtgaaggccc gccgcacgat ggaggccttc gagatctccg agacccgccg
      241 ggtgcgtggg ctgggcgagc agcagaagcg caagctgctc gaggagttcg ggtcctgagc
      301 gccgacggcg cccgtcccgc accgctgccg gcctcgtgaa catcgtcgtg tccggcccgg
      361 gcggggtcgg caagggcacg atcgtcgacc agctcctcgc ccgccgcccc gagatctggc
      421 tgagccggtc ctggaccacc cgcgagcgcc gggcgggcga ggccgaggac gcctacgtct
      481 tcaccgaccg cgagagcttc gacgcccaca tcgacgcggg cggcttcctg gagtgggtcg
      541 acttcctcga ctaccgccag ggcaccccgg tcccgaaccc gccacccggc accgacgtgc
      601 tcctggagat cgacgtggcc ggggccgagg tcatccgcgc cgtggacccc gacgcgctgc
      661 tcgtcttcgt cgacacgccc gaccgcgagg cccagcgcca gcgcctcacc ggtcgcggcg
      721 acagccccga tcgcatcgag gcccgcatgc gcaagggcga cgaggagcgg gtcgcggccc
      781 gccggctggg catgcaccac atcgtcaacg acgtcctcca cgacgccgtc gccgagctcg
      841 aagccctcat cgacgcgcac cgccggcgct gagggtttcc cgggccggcc gggcccgctg
      901 ctagcgtggc tcgttcggca acccctcgga ggcaacatgt ccggtcgtca cgacacgatg
      961 atgaacccgc ggatcgagga cctcctcgat cgcacggatt ccaagttccg tctcgtcacg
     1021 ctgggttcca cgcgggctcg ccagatcaac tcgtacttca acgccgtggg cagcggcggc
     1081 ctcggctccc aggtcccccc gcaggtgccc tcggtcgtgc gcaagccgct gtcgatcgcc
     1141 ttcgaggaga tcgccgccga caagatcctc cccgtctacg gcgaggagct cgccgccaag
     1201 cgcgaggccg aggcagccga ggccgcggcc cgcatcgctg cggccgagga ccagggctag
     1261 tcgctgcccg tcgcggcccg cccatgctcg ccgggaagca catcgtcctc ggggtgaccg
     1321 gcgggatcgc ggcctacaag gccgtcgagg tctgccggcg cctggtcgac gccggtgcgc
     1381 acgtcacacc gatcatgacc gagggcgccc ggcacttcgt cggggagacc acgctgtcca
     1441 ccctggcctc cgagaccgtc cgaacgtcgc tgtgggagat cgaccacggc gctgacggca
     1501 gcccgatcac ccacacccgc ctgggccaga ccgccgacct ggtcatcgtc gccccggcca
     1561 ccgctcgggt gatctcggat ctgcgcaccg gccggtccgc cgatctgctc tcggccacgc
     1621 tgatcgccac caccgcaccg gtgctgctgg caccggcgat gcacaccgag atgtgggagc
     1681 accccgccgt gcgggagaac ctcgaggtgc tcgccacacg cggcgtgcac gtggtgccgc
     1741 ccgaggaggg ccgcctggcc ggcggtgact ccggcgccgg gcgcctggcc gacccggcca
     1801 ccatcgtcga ggccgccgag aaggtgctgg ccggcagcgg tgccgacgcg gacctcgccg
     1861 gtctcgccgt gctcgtcacc gctggcggca cgcgggagcc catcgacccg gtccgcttca
     1921 tcggcaaccg gtcgtcgggc aagcagggtc acgcgatcgc cgaggaggcc gccgcccgcg
     1981 gcgcccgggt caccctcgtc accaccgtcg accgccccgc cagcgccggc gtcgagatcg
     2041 tgcgggtcga caccgctgac gagatgcacg ccgccgtcgc cgagcgggcc tcgtcgagcg
     2101 acgtggtcgt gatggccgcc gccgtcgccg acttcaagcc cgccgccccc gccgaccgca
     2161 agatcaagaa ggccgggggc acccccgagg tcgtcctcga gcccaccgtc gacatcctcg
     2221 ccagcctcgg tgcctccaag cccgccggcc agacgctggt cggcttcgcc gccgagaccg
     2281 acgacctgcg cgccaacgcc caggccaagc tggccgccaa gggcgctgac ctcatcgtcg
     2341 ccaacgacgt gtccgccccc ggcgccggct tcgagcacga caccaacgag gtcgtgctgc
     2401 tgtcggccgg cggtgccacc gaggtgcccc aggccgacaa gcgccagatc gccagggcgg
     2461 tgctcgacgc cgtcatcacc ctccgcagcc cgaatccagg gagccagaca tgaagaacga
     2521 cttcaccttc acctccgagt ccgtctcgga gggccatccc gacaagatgg ccgaccagat
     2581 ctccgacgcc atcctcgatg cgatcctcgc cgaggacccg atggcccgcg tcgcctgcga
     2641 gaccatggtc accaccggca tggccatcgt ctcgggtgag atcaccacca cggcctacgt
     2701 cgagatcccc gacatcgtcc gcaagacgat caacggcatc ggctacgacc gggagtcgat
     2761 gggcttcgac ggcaacacct gcggcgtgat gaccaccatc gacccgcaga gccccgacat
     2821 cgcccagggc gtggacaagg ccttcgagac ccgcaccggc gagcgcgacg acgagctcga
     2881 cgaccagggt gctggcgacc aggggatgat gttcggctac gcgtgcgacg agaacgacgc
     2941 gatgatgccg acgccgatcc acctggccca ccgcctcgcc gagcgcctgg ccgaggtccg
     3001 caaggccggg atcctcccgt acctgcgccc cgactccaag acccaggtca ccatcgacta
     3061 ccgcgacggc cgtccggcgg ccctcgacac cgtgctgatc tccacccagc acgccccggg
     3121 ccacgacgcc gacacgctca tcaagcccga cctgatcgag cacgtgatcc gcccgacgct
     3181 gcccgaggcc ttcgccgacg acgacttccg ggtgctggtc aaccccaccg gaaagttcga
     3241 gctgggtggc ccccacgccg actgcgggct caccggccgc aagatcatcg tcgacaccta
     3301 cgggggctgg gcccgtcacg gtggcggcgc cttctccggc aaggacccgt ccaaggtcga
     3361 ccgctccgcc gcctacgcca cccgctgggt cgccaagaac ctggtggccg ccggcgccgc
     3421 ctcccgctgc gaggtccagg tcgcctacgc catcggcgtc gcccacccga tgtcgatcat
     3481 ggtcgagacc ttcggcaccg agaacgtcga ccccgcccgc atcgaggagg ccgtccgcga
     3541 ggtgttcgac ctgcgccccg gtgccatcct gcgcgacctc gacctgcgcc ggccgatcta
     3601 ccgcaagacc gcggcctacg ggcacttcgg tcgcacgggc gtcgacggcg agttcccctg
     3661 ggagaacacg aaccgggtcg acgacgtgaa gtcggcgctc ggtctctaga ccggcaccgc
     3721 accaccgctg agcaccgacg aacccaccct cttcgggggc gacccaccgg agccgcccga
     3781 tccgacgccc gcaccggcgg gccgggtcgt gcgcgtgctc ccggaccaga ccggcacggc
     3841 caagaccttc gactacctcg tccccgaggc ctgggccgac ggcgtccggg tccaggtcgg
     3901 ctcccgggtc cggatcgcgc tgggcccgcg gcgggtgggg gcctgggtca ccgacgtcga
     3961 cgtcgacccg cccgccggcg tggtgctgaa gcccatcgcc aagctgtccg gccacggacc
     4021 ctcggccgag ctgatcgacc tggcccggtg ggcgcagtgg cgctgggccg cgaagacgcc
     4081 ggtgccgttc ctgcgcaccg cctcaccgcc tcgcaacgtc gccgggctcc cggccggccc
     4141 ggcccggacg caaccgctgc cccaggtcgc cgacccggtg gtcgcgccgc tcctcgacga
     4201 tgctctggcc ggcggtccgg tcgtgctgcg cctgcccccc acggcggacc tcgccgccgt
     4261 ggccctcggt gcggcccggg caggtgacgc gctggtgatc tgcccgtcgc accggatggc
     4321 ccggcacctc gccgtgcggc tgcgtcgagc cggcgtcgcc gtcgcactgc accccgacga
     4381 gtgggcccgg gcggctgcgg gcggctgcac ggtgatcggg acccgcgcct cggcgtgggc
     4441 gccggtgccg gacctggccg ccgtcgtcgt cctggacgag cacgacgagg tccatcagga
     4501 ggagcgctcg cccacctggc acgcccgcga cgtcgccctc gagcgggccc gccgccgggg
     4561 cgtgccgtgc gtgctcacct ccccgatgcc caccctcgag gcgctgccgg tcgcccgcct
     4621 gatccgcacc gaccgggcca ccgagcgctc cggttggccg gccgccgtcg tggccgaccg
     4681 cacggaggag ccgccgggtc gcaacgcgct gttctccccg cagttggtcg acgtcatccg
     4741 cagcggtgcc cgggtgctgt gcgtgctcaa ccagaagggc cgcgccgccc tgctcggctg
     4801 cgtcggctgc ggcgagatcg tgcgctgcga cgcgtgccag gccgcggtgg ccaagcccgg
     4861 cgacgagctc gtctgccggc gctgcgggac gacgcgtccg gtcatctgcg ccacgtgcgg
     4921 gagcatcgac ctcaaggtca tccgcatggg cgtcaaccgg gtggtcgagg acctcgccgc
     4981 cctcagcggg gagcgggtcg tggccgtcac cgccgagacc cccgccgccg acgtcgacag
     5041 cgcccgcctc tacgtcggca ccgaggccgt gctccaccag gtcggccgcg ccgacgtcgt
     5101 cgccttcctc gacctcgacc aggagctgtt cgcgccccgg taccgggccg ccgaggaggc
     5161 gctcggcctc gtggttcgcg ccgcccggat cgtcggcgga cgcggggagg gcgggcgcgt
     5221 gctgctccag acccgctcgc ccgagcacga ggtgtgccgg gccgccctgc tcggcgaccc
     5281 cgagcgggtc gcgtcggccg aggacgagcg ccgggcgatg ctcgggttcc cgccccacgt
     5341 cgccctcgcc gaggtgggcg accaggcggg cgaggcgttc gtcgccgcgc tcaccgggtc
     5401 ggccccaccc gacgtgcagg tgcagggccc cgtggacggg gcgtggcgcg tccgggcccc
     5461 cgaccaccag acgctctgcg acgcgctcgc cgccgtgcac cgcccgccgg gacgcctgcg
     5521 cctgtcggtc gaccccctgc ggatctaggc gcgctgcgac cggagcaggc ggtagccgcg
     5581 cttggaggcg aggcgctcgg tggggaagcc ctgctcgccc agccagcgct gcatcgagtc
     5641 cgccccgagg ttccggccca tcaccaggac ggcctccccg tcgggggtga gcaggtcgag
     5701 ccagcggagc agcagctcgc ggagcgcgtc cttgccgatg cggatcggcg ggttcgacca
     5761 gatgaggacg aagcggaggg cggggtcgac atcgtcgggg gcgagcacct cgacgttggt
     5821 gcagccgagg tcggtggcgt tccgggcgca cagctcgcga ctgcgctcgt tgacgtccac
     5881 ggcgacgacg cgggcgtcgg ggctcgtccg ggcgagcacg gcggcgatc
//
LOCUS       DATISU010000233         5426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_757466, whole genome shotgun sequence.
ACCESSION   DATISU010000233 DATISU010000000
VERSION     DATISU010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757466"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VLR27_09465"
     CDS             <1..478
                     /locus_tag="VLR27_09465"
                     /inference="COORDINATES: protein motif:HMM:NF025792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HSP03717.1"
                     /translation="GRELVFGTVTGSAVGGGAHRLTSGLGSTVDDLVPARSTAALDHA
                     PTAPPAGVSGGGDLVNLASDVRTDHILSGHMPPGQPGKTLFPDTWSADQIMHHVSDIA
                     TDPSYTWVQQTGKPGAEFTRNGDPVRYFVDGVRDDVPVRVILEPGGEGIVTAFPTP"
     gene            475..753
                     /locus_tag="VLR27_09470"
     CDS             475..753
                     /locus_tag="VLR27_09470"
                     /inference="COORDINATES: protein motif:HMM:NF033691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HSP03718.1"
                     /translation="MTCAGDDQDVASRLRRLLAQLEGLPEGDVESIGVLIDAGEWTVA
                     LENLCTQAYEYDVEPAEPIRREIHELGERLDVHTAYLLGDPWADRDGR"
     gene            758..1138
                     /locus_tag="VLR27_09475"
     CDS             758..1138
                     /locus_tag="VLR27_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03719.1"
                     /translation="MRIRVVGVTDAAGRVVECTTPVGRLHVVWESASLPSPGDHDVEL
                     DLPTVLEWGCEVWTATDREPAHDPGRPLLSGTLLGVDEAGVAEVRVPGGLLTVETSGE
                     PPLGAIGEAVELQPPRVLAYPFDL"
     gene            1182..2405
                     /locus_tag="VLR27_09480"
     CDS             1182..2405
                     /locus_tag="VLR27_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSP03720.1"
                     /translation="MSTRNEIHVIGAGLAGLTAAAFVARAGLPVVVHESRDRLGGRAT
                     TDERDGFRFDQGPHALYRGGAAERVLAELGIQPRGSAPDVRGVVVRSGRLHRAPAGAP
                     SLLGTTALGWRGKLQLARLLQRLPRLRPAEHAHRTAEEWTEATVRDARARELLAMLVR
                     LTTYANHPRAMSADVAIQMLQAGLGPGVRYLERGWDQLVDALAATPGVRIAEGGTLRE
                     LPDAAAVVLATGSPAAAAGLAGVPVDRLAVGPAAEVACLDLGIRGEPPHPVALGLDVP
                     IYASHHSAPGGRAPEGHSVVALAEYLAPGVEPSSERLDAFAGTMGIARESVVARRYLH
                     RMVSCTAIPVASHGGLAGRPDVDVLARLADRPGTYVAGDWVGPEGHLADAVLASARAA
                     ALAAVHHVERSPVPR"
     gene            2402..3298
                     /gene="sigJ"
                     /locus_tag="VLR27_09485"
     CDS             2402..3298
                     /gene="sigJ"
                     /locus_tag="VLR27_09485"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HSP03721.1"
                     /translation="MTPDADTRSTEVFEAERARLAGLAYRMLGTPDDADDVVQDAWLR
                     WAAADRSAIVEPAAWLTTVTTRLAIDRLRSARHRREVYVGPWLPEPLLAGDDDPLASI
                     ATAESLSLAFLTVLERLEPVERAVFLLHDVFGYPFADVAAHVERSEAAARQIAKRARD
                     RVQAERPRMDADPTHAEELAEAFLGAVVQGDVDGLRRLLTDDVVHLSDGGAHRRAARR
                     PVIGADRVARLLVNLASRMEPGTRVDPVRVNGQLGWYVTVEGRPELVLVPSFRDGRVA
                     SVLAVLNPDKLARFHAAWLAQS"
     gene            complement(3365..4510)
                     /locus_tag="VLR27_09490"
     CDS             complement(3365..4510)
                     /locus_tag="VLR27_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HSP03722.1"
                     /translation="MTDTTASSENAAIVGREDLHDIEGILSVVGSDEGELIRAVQDNA
                     DALFTWNYEKGERPALNRLYEKAKTSMWNGETDLDWSIEVDQEAVVVANAAGAGGLGP
                     EMDVSGTPFATWGEKEWLQLGVESQNWMLSQFMHGEQGALLCTAKIVETVPWIDAKYY
                     AATQVMDEARHVEVFAKYLDTKLSGHYPLNAHLGLLLDDIIEDSRWDMTYLGMQIMVE
                     GLALAAFGFIHQMTTEPLLKKLLRYVMSDEARHVAFGVLSLKEYYAELSDAEMKERQE
                     FAFEAAVRMRDRFLQQEVWARMGLDVKEVLPLVMAAPERAMFQSMLFAKIVPNCKKLG
                     LLDRNDGWLRQKFTELGVIQFEDWADTEEEYEMFALAEGEVNSSNQG"
     gene            complement(4554..5180)
                     /locus_tag="VLR27_09495"
     CDS             complement(4554..5180)
                     /locus_tag="VLR27_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP03723.1"
                     /translation="MGTISTTDAPGLAAPVATATEERIRTAALACIARTGITRTTVDD
                     VVREAGVSRATLYRAFPGGKDVLVEAVLGREVHRFFVELAGELARHDDPADLLAAGVG
                     RALRFLTEHPALRAVLAHEPGLLLSQVAFHRLGPILDAAAAFAAPYLRPHVRADHPRP
                     DDAATEVAELLVRVVLTYALEPSPHVDPHDEVSLDRFVRRHLLPALTA"
     gene            complement(5242..>5426)
                     /locus_tag="VLR27_09500"
     CDS             complement(5242..>5426)
                     /locus_tag="VLR27_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03724.1"
                     /translation="GEAVARGEVRSDVDVAMAGDHAARLVLSYVGSPGRWDLVDPDEA
                     RRLVQDRVLAGVVPGG"
BASE COUNT          700 a         1966 c         1983 g          777 t
ORIGIN      
        1 cgggcgcgag ctcgtcttcg gcacggtcac cgggagcgcg gtgggcggcg gcgcccaccg
       61 cctcacgtcc ggcctggggt cgaccgtgga cgacctggtg ccggctcgct cgaccgccgc
      121 gctcgaccac gcgccgaccg ccccgcccgc tggcgtctcc ggtggcggcg acctcgtcaa
      181 cctggccagc gacgtgcgga cggaccacat cctgtccggc cacatgccgc cggggcagcc
      241 gggcaagacg ctgttcccgg acacctggtc ggcggaccag atcatgcacc atgtctccga
      301 catcgccacc gacccgtcgt acacgtgggt gcagcagacc gggaagccgg gcgcggagtt
      361 caccaggaac ggtgacccgg tgcgctactt cgtcgacggc gtacgggatg atgtcccggt
      421 gcgcgtgatc ctcgaaccag gtggcgaggg gatcgtcacc gccttcccga cgccgtgacc
      481 tgcgccggcg acgaccagga cgtcgcgtcg aggctccggc ggctgctcgc ccagctggag
      541 ggcctgccgg agggggacgt ggagtcgatc ggcgtcctca tcgatgccgg tgagtggacc
      601 gtggcgctgg agaacctctg cacgcaggcc tacgagtacg acgtcgagcc cgccgagccc
      661 atccgccggg agatccacga gctcggcgag cggctggacg tgcacacggc gtacctgctc
      721 ggcgacccgt gggcagaccg agacggccgt tgacgctgtg cgcatccgcg tcgtaggcgt
      781 caccgatgct gccggccggg tcgtcgagtg cacgaccccc gtcggccggc tccacgtggt
      841 ctgggagtcg gcgtcgctgc cgtcacccgg tgaccacgac gtggagctgg atctgcccac
      901 ggtcctcgag tgggggtgcg aggtgtggac cgccaccgac cgggagcccg ctcacgatcc
      961 gggtcgaccg ctgctctccg ggaccctgct cggggtggat gaggcaggcg tggccgaggt
     1021 gcgggtgccc ggtggcctcc tgaccgtcga gacctcgggc gagccgccgc tcggtgcgat
     1081 cggggaggcg gtcgagctgc agccaccccg cgtcctcgcg tacccgttcg acctgtaggg
     1141 cacggctgtc acatccgcga cggctgcgtc gtcgagtggg catgagcacg aggaacgaga
     1201 tccacgtcat cggcgccggc ctcgccgggc tgaccgccgc cgccttcgtc gcccgggccg
     1261 ggttgccggt ggtggtgcac gagtcccgag accggctggg cggccgcgcc accaccgacg
     1321 agcgcgacgg cttccgcttc gatcaggggc cccatgccct ctaccggggt ggggcggcag
     1381 agcgggtgct cgcagagctg ggcatccagc cccgagggag cgcaccggac gtgcgcggcg
     1441 tggtggtgcg cagcggtcgc ctgcaccggg ctcccgccgg cgccccatcg ctgctgggta
     1501 cgaccgcgct gggctggcgg ggcaagctgc agctggcccg cctcctgcag aggctgccgc
     1561 gcctgcgtcc ggccgagcac gcccaccgca cggcggagga gtggaccgag gccaccgtcc
     1621 gggacgcccg agcgcgtgag ctgctcgcca tgctcgtccg gctcaccacc tacgccaacc
     1681 acccccgggc gatgagcgcc gacgtggcga tccagatgct gcaggccggg ctcggcccgg
     1741 gcgtgcggta cctggagcgg ggatgggacc agctcgtcga cgccctggcc gccacccccg
     1801 gcgtgcgcat cgccgagggc gggaccctcc gggagctccc cgacgccgct gccgtcgtcc
     1861 tcgccaccgg ctcccccgcc gccgcagctg gcttggccgg cgtgccggtc gaccgcctcg
     1921 cagtcgggcc ggcggctgag gtggcgtgcc tcgacctcgg catccgcggc gaaccgccgc
     1981 accccgtcgc cctcggtctc gacgtcccga tctacgcatc gcaccactcc gcccccggcg
     2041 gtcgagcgcc ggaaggtcac tcggtggtgg cgctggccga gtacctcgcc cccggggtcg
     2101 agccgtcgag cgagcgcctc gacgccttcg ccggcaccat gggcatcgca cgggagtccg
     2161 tcgtggcccg gcggtacctt caccgcatgg tgtcgtgcac ggcgatcccc gtcgcgtcgc
     2221 acggcgggct ggcgggtcgg cccgacgtcg acgtgctggc acggctggcg gatcgtccgg
     2281 gcacgtacgt cgccggcgac tgggtcggtc ccgagggcca cctggccgac gcggtgctgg
     2341 ccagtgcccg cgccgctgca ctcgccgccg tgcaccacgt cgagcggtcg ccggtgccga
     2401 ggtgaccccc gacgccgaca cgcggtccac cgaggtcttc gaggccgagc gcgcccggct
     2461 ggctggcctc gcctaccgga tgctcggcac ccccgacgac gccgacgacg tcgtgcagga
     2521 cgcgtggctg cggtgggcgg cggccgaccg ctcggcgatc gtcgagcctg cggcgtggct
     2581 gacgaccgtc acgacccgcc tcgccatcga ccggttgcga tcggcccgcc atcgccggga
     2641 ggtgtacgtc ggcccgtggc tgccggaacc gctcctcgcc ggagacgacg acccgcttgc
     2701 ctcgatcgcc accgccgagt ccctcagcct cgcgttcctc accgtgctcg agcgcctgga
     2761 acccgtcgag cgcgccgtct tcctgctcca cgacgtcttc ggctacccct tcgccgacgt
     2821 ggccgcccac gtcgagcgct cggaagcggc agcccgacag atcgccaagc gagctcgtga
     2881 ccgcgtccag gccgagcgcc cgcgcatgga tgccgacccg acccacgccg aggagctggc
     2941 cgaggccttc ctcggtgcgg tggtccaggg ggacgtcgac ggcctccgac gcctgctcac
     3001 cgacgacgtc gtgcacctct ccgacggcgg cgcccatcgc cgggccgctc gacggcccgt
     3061 gatcggcgcg gaccgggtcg cccgcctgct cgtcaacctc gcatcccgga tggagccggg
     3121 cacccgggtc gacccggtgc gggtgaacgg ccagctcggc tggtacgtga ccgtcgaggg
     3181 ccgccccgag ctggtgctcg tcccgtcctt ccgagacggt cgggtcgcca gcgtcctcgc
     3241 cgtgctgaac cccgacaagc tcgcccgctt ccacgccgcc tggctggcgc agtcgtaatc
     3301 gcgcccctgg gcgacgattc gtcgacgaat cgtcgcccag tgctccgggg tcggatgcgg
     3361 caggtcagcc ctggttggag ctgttcacct cgccctcggc gagagcgaac atctcgtact
     3421 cctcctcggt gtcggcccag tcctcgaact ggatgacgcc gagctcggtg aacttctggc
     3481 gcagccagcc gtcgttgcgg tcgagcaggc cgagcttctt gcagttcggg acgatcttgg
     3541 cgaacagcat cgactggaac atggcccgct ccggcgcggc catcaccagg ggcaggacct
     3601 ccttgacgtc gaggcccatg cgcgcccaca cctcctgctg caggaaccgg tcgcgcatgc
     3661 gcacggcggc ctcgaaggcg aactcctggc gctccttcat ctccgcgtcg gacagctcgg
     3721 cgtagtactc cttcagcgac agcacgccga aggccacgtg gcgggcctcg tcggacatga
     3781 cgtagcggag cagcttcttg agcagcggct cggtggtcat ctggtggatg aagccgaagg
     3841 cggcgagggc gaggccctcg accatgatct gcatccccag gtaggtcatg tcccagcggc
     3901 tgtcctcgat gatgtcgtcg aggagcaggc cgaggtgggc gttgagcggg tagtggcccg
     3961 acagcttcgt gtcgaggtac ttggcgaaga cctcgacgtg gcgggcctcg tccatcacct
     4021 gggtggcggc gtagtacttg gcgtcgatcc acgggacggt ctcgacgatc ttggccgtgc
     4081 agagcagggc gccctgctcg ccgtgcatga actgcgacag catccagttc tgggactcga
     4141 cgccgagctg cagccactcc ttctcgcccc acgtggcgaa cggggtgccg ctcacgtcca
     4201 tctccgggcc gaggccgccg gcgccggcgg cgttggcgac gaccacggcc tcctggtcga
     4261 cctcgatgga ccagtcgagg tcggtctcgc cgttccacat cgaggtcttg gccttctcgt
     4321 agaggcggtt cagcgccggg cgctcgccct tctcgtagtt ccaggtgaac agcgcgtcgg
     4381 cgttgtcctg gacggcgcgg atcagctcgc cctcgtcgga cccgacgacg gagaggatgc
     4441 cctcgatgtc gtgcaggtcc tcgcggccga cgatggcggc gttctcgctg gaggcggtgg
     4501 tgtcggtcat ggggggctcc tgtgggtctt gcgggttcgg gtgggctgga cggtcaggcg
     4561 gtgagggccg gcagcaggtg ccggcgcacg aagcggtcga gggagacctc gtcgtggggg
     4621 tccacgtggg ggctgggctc gagcgcgtag gtgagcacga cccggacgag cagctcggcc
     4681 acctcggtgg cggcgtcgtc cggccggggg tggtcggcgc ggacgtgagg tcgcaggtac
     4741 ggcgcagcga acgccgcggc ggcatcgagg atggggccga ggcggtggaa ggcgacctgg
     4801 gacagcagca ggccgggctc gtgggccagc acggctcgca gcgccgggtg ctcggtgagg
     4861 aagcgcagcg cccggccgac gccggcggcc agcaggtcgg cggggtcgtc gtgacgggcc
     4921 agctcgccgg cgagctcgac gaagaagcgg tgcacctccc ggccgaggac ggcttcgacc
     4981 agcacgtcct tgccgccggg gaaggcgcgg tacagcgtgg cccgggagac cccggcctca
     5041 cggacgacgt cgtcgacggt ggtgcgggtg atcccggtgc gggcgatgca ggcgagagcg
     5101 gcggtgcgga tgcgctcctc ggtggccgtc gccaccggag ccgcgagccc cggtgcatcg
     5161 gtcgtcgaga tcgtccccat caccatgaga cactacgggc cgatctgtct cactgtctac
     5221 gcaaatcgga cgtgacgtcc gtcaccctcc gggcaccacc ccggccagga cccggtcctg
     5281 caccagccgc cgggcctcgt cggggtcgac cagatcccag cggcccgggc tgccgacgta
     5341 gctgagcacg aggcgcgcgg cgtggtcgcc ggccatggcg acgtcgacgt cggaccggac
     5401 ctcgccccgg gccaccgcct ccccga
//
LOCUS       DATISU010000234         5408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_478449, whole genome shotgun sequence.
ACCESSION   DATISU010000234 DATISU010000000
VERSION     DATISU010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478449"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VLR27_09505"
     CDS             <1..301
                     /locus_tag="VLR27_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03725.1"
                     /translation="EHGIPYRDLCFLGQKPEVQADIYVDDAPHNVEALRAAGNDVITF
                     AQPYNAHLADPRAATWEEVESIVLARLVARGAVVEQTLPGFDEGAERLRRRREGR"
     gene            complement(366..1070)
                     /locus_tag="VLR27_09510"
     CDS             complement(366..1070)
                     /locus_tag="VLR27_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HSP03726.1"
                     /translation="MPTPADDVPDLLTGLATTRAIRRYRDEPIPEDDLASILWHATRA
                     PSGSNRQPFRFVVLRDGPRATEARQLLGTSFRQLWAAKRRSDGYDEGSGAEADTPKAR
                     MAATMQHFVDHFEATPVVVLACLRRHRDPNPTEGASVYPACQNLLLAARALGYGGVLT
                     MWHQYVEDELRDVLGIPADVAISATITLGRPVGHHGPVRRRPVAELVYEDGWEEPAPW
                     AEDPPGTRFTSAGPPR"
     gene            1069..2214
                     /locus_tag="VLR27_09515"
     CDS             1069..2214
                     /locus_tag="VLR27_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03727.1"
                     /translation="MGASIAAVLTSLDDYPIHQTSAPLHQPVSGDRNHYDRYFFNGYD
                     REGSLFFALAMGRYPNRSVLDAAFSVVDGGVQRSVHASSRAPLDPIDTSCGPVRVEVI
                     EPMQVLRVVVDADVHGIGCDLTWRARTVAVEEDRFRRMNGTRIVMDYTRLTQFGTWEG
                     WIRTGDREVAIEPGTFLGSRDRSWGIRGVGEPEGGAPGTSLPQFFWLWAPLNFEDGAA
                     HFDVNEDARGDRWHQTAIRIPLLADGASPIDPGGRSDSAEPMRTVDWHIDWEPGTRRA
                     RHAELTFTPWNEDPSTIELEPLLTFQMLGIGYFHPDWPHGVWKGESESAVDELVLADL
                     DPALAQHTHVQQLVRATWGGRTGIGVLEQLAIGDHAPSGLHGLFDGA"
     gene            2282..2482
                     /locus_tag="VLR27_09520"
     CDS             2282..2482
                     /locus_tag="VLR27_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005511370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HSP03728.1"
                     /translation="MATGTVKFFNNEKGYGFISRPDGEDVFVHFSNIEGSGFKTLEEG
                     QNVEFEVGPGRKGDEAVNVRVI"
     gene            2576..3334
                     /locus_tag="VLR27_09525"
     CDS             2576..3334
                     /locus_tag="VLR27_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HSP03729.1"
                     /translation="MTPISVGIQLHPQATTLDELRAAARAADEMGADSIWVWDHFFPL
                     YGDGDAAHFECYTTLAALAVETERARLGALVTCNSYRNPNLLADMARTIDHLSHGRFT
                     LGIGSGWFERDYDEYGYEFGTAIGRLRALERDLPIIKDRLAKLNPGPVGPMPIMIGGS
                     GRKVTLRIVAEQATSWNTFGTPDEFAETNAVLDEWCAKVGRDPSEIERTVAVDPKAAG
                     QVQEYAGAGADHLILMQGPPFPLDALQTALDTRG"
     gene            3380..4624
                     /locus_tag="VLR27_09530"
     CDS             3380..4624
                     /locus_tag="VLR27_09530"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HSP03730.1"
                     /translation="MTSAPASQSARGHRCRFAVATVLAVLVALVPMTPASAQTFSDVS
                     GPHAGAIEAMAEAGITDGCTPERYCPDEPVTRAQMASFLMEALDLSPEPAPFTDVEGN
                     EHEGAIGAIAASGITDGCTAERYCPAEPVSRAQMASFLARGFGMSDPGETYFHDVTAP
                     HGANAQALAAAGVSDGCVALGLRFCPQKTVSRAQMASFIARSLDLVPRTSVPLRLDPG
                     EQNAHVEALQNLLAWHGYWVGPVDGVYGHLTEQAVLAVQKVHGLARDGIYGPSTRAAM
                     AHLVPPTTRSSSGYVIEFDEDRQVVMLVRDGHPETIFHASGGDESYYTSDGTTYWAET
                     PNGTWDIYRQIDGWRTSHLGELYRPKYFHTDGIAFHGYDVVPPYAASHGCVRVSMEAM
                     DWIWATNAMPIGATVMVYGTPS"
     gene            complement(4632..>5408)
                     /locus_tag="VLR27_09535"
     CDS             complement(4632..>5408)
                     /locus_tag="VLR27_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HSP03731.1"
                     /translation="SVQESAIDGSFRLESNHGALQGAAMAEIFDRFETAELHADWAAA
                     REVHGDDARVEHLDRTPAQRRADALFQIFRRAAATQGCSPEPLVNVIIDQDTFEDELT
                     RTAGLDVRVDPNQVLDSRRCHTLDGTQIRPAEAVAAALVGHVRRVVVDGKGNVIDLGR
                     RQRLFTGSARDAAFLQALLRGPGGLGCLWPGCDGRGRNLQVDHRDPACRGGPTNTDNA
                     DAYCGFHNRTKEHGFRPVRDPDGTWTIHRPDDDGPITPPV"
BASE COUNT          785 a         1977 c         1872 g          774 t
ORIGIN      
        1 cgagcacggc atcccctacc gcgacctgtg cttcctcggc cagaagcccg aggtgcaggc
       61 cgacatctac gtcgacgacg ccccccacaa cgtcgaggcg ctccgggccg ccggcaacga
      121 cgtcatcacc ttcgcccagc cttacaacgc ccacctcgcc gatccccgcg ccgccacctg
      181 ggaggaggtg gagagcatcg tgctcgcccg cctcgtcgcc cggggcgccg tggtggagca
      241 gaccctgccc ggcttcgacg agggcgccga gcgcctccga cggcgccgcg agggccgctg
      301 agcggaccaa cccgttcctt gcgcgcagat cgaccgtgtg ttgtcgatcc gcgcgtcgag
      361 aaccactacc gaggcgggcc ggccgaggtg aagcgggtgc cgggcggatc ctccgcccac
      421 ggcgccggct cctcccaccc gtcctcgtag acgagctcgg cgaccggccg gcgacggacc
      481 gggccgtggt gcccgaccgg gcggccgagc gtgatggtgg cgctgatcgc cacgtcggcc
      541 gggatcccga gcacgtcgcg cagctcgtcc tccacgtact ggtgccacat ggtgaggacc
      601 ccgccgtagc ccagggcccg ggcggccagc agcaggttct ggcaagccgg gtagaccgat
      661 gctccctccg tcgggttcgg gtcccggtgg cggcgtaggc aggcgagcac caccacgggc
      721 gtggcctcga agtggtcgac gaagtgctgc atggtcgccg ccatgcgcgc cttcggcgtg
      781 tcggcctccg cgccgctccc ctcgtcgtag ccgtcgctgc ggcgcttggc cgcccagagc
      841 tgccggaagc tcgtgccgag cagctggcgc gcctcggtcg cccggggccc atcccgcagg
      901 acgacgaacc ggaacggctg gcggttgctg cccgacgggg ctcgggtcgc gtgccacagg
      961 atgctcgcca ggtcgtcctc cgggatgggc tcgtcccggt agcgccggat ggcccgggtg
     1021 gtggcgagcc cggtcagcag gtcgggcaca tcgtccgcgg gggtcggcat gggtgccagt
     1081 atcgctgccg tgctcaccag cctcgacgac taccccatcc accagacctc ggcgcccctg
     1141 caccagccgg tgtccggcga tcgcaaccac tacgaccgat acttcttcaa cggctacgac
     1201 cgcgagggct cgctcttctt cgccttggcg atgggccgct acccgaaccg ctcggtcctc
     1261 gacgccgcgt tcagcgtggt cgacggaggg gtccagcgca gcgtccacgc ctcgagccgg
     1321 gcaccgctgg acccgatcga caccagctgc gggccggtgc gcgtggaggt catcgagccg
     1381 atgcaggtcc tgcgggtggt ggtcgacgcc gacgtccacg gcatcgggtg cgacctgacg
     1441 tggcgggcgc gcaccgtcgc cgtcgaggag gaccggttcc ggcggatgaa cgggacccgg
     1501 atcgtcatgg actacacccg cctcacccag ttcgggacgt gggagggctg gatccgcacc
     1561 ggtgaccgcg aggtggccat cgagccgggc acgttcctcg gctcccgcga ccggtcctgg
     1621 ggcatccgcg gcgtcggcga gcccgagggc ggcgcccccg gcacctcgct cccgcagttc
     1681 ttctggctgt gggcgccgct caacttcgag gacggcgccg cccacttcga cgtcaacgag
     1741 gacgcccgcg gcgaccggtg gcaccagacc gccatccgga tcccgctgct cgccgacggc
     1801 gcctcgccga tcgaccccgg cggccgcagc gactcggccg agccgatgcg gaccgtggac
     1861 tggcacatcg actgggagcc gggcacccgg cgggcccgcc acgccgagct gacgttcacg
     1921 ccgtggaacg aggacccctc caccatcgag ctcgagccgc tgctcacctt ccagatgctg
     1981 ggcatcgggt acttccaccc ggactggccc cacggcgtct ggaagggcga atccgaatcc
     2041 gccgtcgacg agctggtcct ggccgatctc gacccggcgc tggcccagca cacccacgtg
     2101 cagcagctgg tccgggcgac ctggggcggt cgcacgggca tcggtgttct cgagcagctg
     2161 gccatcggcg atcacgcgcc cagcggcctc cacggcctct tcgacggggc ctaggctctc
     2221 tgccatcgaa ttggaatcgg gggaggcgcg cggcgccgaa cccaagcgaa cggagcaagc
     2281 gatggcgacc ggaacggtca agttcttcaa caacgagaag gggtacggct tcatctcccg
     2341 ccccgacggc gaggatgtgt tcgtgcactt ctccaacatc gaaggatcgg ggttcaagac
     2401 cctcgaggag ggccagaacg tcgagttcga ggtcggtccc ggccgcaagg gcgacgaggc
     2461 cgtgaacgtc cgggtgatct gaccggttcg gatcaacgct cgtgagagac gacggcgccg
     2521 gggcaccagc cccggcgccg tcgtcgttct cgggcgccgt ggatagcgtc cggccatgac
     2581 ccccatcagc gtcggcatcc agctccaccc ccaggcgacg accctcgacg agctgcgggc
     2641 tgcggctcgc gccgccgacg agatgggggc ggactcgatc tgggtgtggg accacttctt
     2701 cccgctctac ggcgacggcg acgcggccca cttcgagtgc tacacgacgc tggccgccct
     2761 ggccgtcgag accgagcggg cccgcctggg tgcgctggtc acctgcaaca gctaccgcaa
     2821 cccgaacctg ctggccgaca tggcccgcac catcgaccac ctctcgcacg ggcggttcac
     2881 cctcggcatc gggtcgggct ggttcgagcg ggactacgac gagtacggct acgagttcgg
     2941 caccgccatc ggccgcctcc gcgccctcga gcgcgacctg cccatcatca aggaccgcct
     3001 ggcgaagctg aaccccgggc cagtcgggcc catgccgatc atgatcggcg gctccggccg
     3061 caaggtcacc ctccgcatcg tcgccgagca ggcgacgtcg tggaacacct tcggcacccc
     3121 cgacgagttc gccgagacca acgctgtcct ggacgagtgg tgcgccaagg tcggccggga
     3181 cccctccgag atcgagcgga ccgtcgcggt ggacccgaag gccgccggcc aggtgcagga
     3241 gtacgccgga gccggtgccg accacctgat cctcatgcag ggcccgccct tcccactcga
     3301 cgccctgcag accgcgctcg acacccgcgg ctgaggagcc gccggggtcg ggacgccggg
     3361 gtgtgggtac gtcgcaagca tgacctctgc gccagccagc cagtccgctc ggggtcaccg
     3421 gtgccggttc gccgtggcca cggtcctcgc cgtgctcgtg gcattggtgc cgatgacccc
     3481 cgcttccgcc cagaccttct ccgatgtctc cggcccccat gccggagcga tcgaggcgat
     3541 ggctgaggcc ggcatcaccg atggctgcac ccccgagcgc tactgcccgg acgagccggt
     3601 cacgagggca cagatggcgt cgttcctgat ggaggcgctg gacctgagcc cggagccggc
     3661 cccgttcacc gatgtggagg gcaacgagca cgagggcgcc atcggcgcca tcgccgcgtc
     3721 gggcatcacc gatggctgca ccgccgagcg ctactgcccc gccgaaccgg tgtcccgagc
     3781 gcagatggcg agcttcctcg ctcgaggctt tggcatgagc gaccccggtg agacgtactt
     3841 ccacgacgtc accgcgccgc acggagcgaa cgcgcaggcg ctggcagcgg ccggcgtcag
     3901 cgacggttgc gtcgccctcg gtttgcgctt ctgcccccag aagacggtgt cccgggccca
     3961 gatggcgagc ttcatcgccc gctccctcga cctcgtgccc cgcacgtcgg tccccttgcg
     4021 cctcgacccc ggcgagcaga acgcccacgt cgaagcgctc cagaacctgc tcgcctggca
     4081 cggctactgg gtcggtccgg tcgacggggt ctacggccac ctgaccgagc aggccgtgct
     4141 cgccgtccag aaggtgcacg gcctggcgcg cgacggcatc tacgggccca gcacccgtgc
     4201 cgccatggcc cacctcgtcc caccgaccac ccgcagctcg tcgggctacg tcatcgagtt
     4261 cgacgaggac cgccaggtcg tcatgctggt ccgcgacgga caccccgaga cgatcttcca
     4321 cgcctcgggc ggtgacgaga gctactacac gagcgacggg acgacctact gggccgagac
     4381 cccgaacggc acgtgggaca tctaccgcca gatcgacggg tggcgcacct cgcacctcgg
     4441 cgagctctac cgccccaagt acttccacac cgacggcatc gccttccacg gctacgacgt
     4501 ggtcccgccc tacgccgcct cgcacggctg cgtgcgcgtc tccatggagg ccatggactg
     4561 gatctgggcg acgaacgcga tgccgatcgg cgccacggtc atggtgtacg gaacgcccag
     4621 ctgacactgg ttcagacggg tggggtgatg ggtccgtcgt cgtcggggcg gtggatggtc
     4681 caggtgccgt cggggtcgcg aaccgggcgg aagccgtgtt ccttggtgcg gttgtggaac
     4741 ccgcagtacg catccgcgtt gtcggtgttg gtgggtccgc cccggcaggc cgggtcgcgg
     4801 tggtcgacct gcaggttcct gccccgtccg tcgcagcccg gccacaagca cccgaggcca
     4861 ccgggtcctc gcaggagggc ttggaggaaa gcggcatcgc gggcggagcc ggtgaacaac
     4921 cgctgccggc gaccgagatc gatcacgttc cccttcccgt cgaccaccac gcgccgcaca
     4981 tggccgacga gggcggcagc gaccgcttcg gccgggcgga tctgggtgcc gtcgagggtg
     5041 tggcagcgcc ggctgtcgag cacctggttc gggtcgaccc ggacgtccag gccggcggtt
     5101 cgggtgagct cgtcttcgaa ggtgtcctgg tcgatgatga cgttgaccaa tggttccggg
     5161 gagcagcctt gggtggctgc ggcccggcga aagatctgga atagggcgtc ggctcggcgt
     5221 tgggcggggg tgcggtcgag gtgctcgacc cgggcgtcgt cgccgtggac ctcgcgggcg
     5281 gcggcccagt cggcgtggag ctcggcggtc tcgaaccggt cgaagatctc cgccatcgcc
     5341 gccccctgca acgccccatg gttgctctcg agccggaacg acccatcgat ggccgactcc
     5401 tgcaccga
//
LOCUS       DATISU010000235         4735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_105739, whole genome shotgun sequence.
ACCESSION   DATISU010000235 DATISU010000000
VERSION     DATISU010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105739"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2743
                     /locus_tag="VLR27_09540"
     CDS             <1..2743
                     /locus_tag="VLR27_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSP03732.1"
                     /translation="TGRLRAFRRGPDGVEVEVGEVGRAEMAGEMALLEDLPRGATVYA
                     VRDSHLVRFSRAAFSAVITRYPSAGLMVAQTVLRRASKRDDRPQRLSVAVIGGAGEVD
                     VDGFARQLAESLGSEARYVTSADIDAELGVDGLAQVEDDEVGSVRLAYWLEELQERNR
                     FIVYGVDETWSTWSRRALRWSDHVLVVADAEADPKPTPFEAEMWRLVEQQKHPKVSLA
                     LLHPADTELPSGTPRWLEPRHLSSHHHVRMGDQVTVDRLARLLAGRGTSLVLGGGGAR
                     GFAHLGVLVVLDGTGVPIDMVGGTSIGSIMAAGPAMGWSAEKSRMLAMEQFRKIFDYT
                     LPAVAVLKGERVTTKLRKAFGDVDISDLWIPYFCVSTNLTTAGAHYHDRGPLVDAIRA
                     SVALPGVLPPVPFEGELLVDGGILDNVPVDEMRRRNTTGRVIAIDVAPAEGPSVTHDY
                     GLSMSGFRALLDRRRGDGPPAMLATLVRSSLIASVRDRDRVVRDRVADLYLDVTVDGA
                     GLLDFDAAEHIAANAESITRPIIRRWVQSTDPKELSYVQTTASAGTVLSDVSARARVA
                     GMILLTLRDLQHRAVRFAAVIAGTAVVLALLFLMTGLIEQFHLEPEDTVDALGAESWL
                     LRDGVSGAFTAASTMPADTADALGDGAAPVVVARHSIAGASEQTDVVIIGYQQGELGE
                     PPLDDGALPANDREVLIDDSAGVSPGEEIALGGEQYLVSGTTDRTTMFAGMPLVFMDI
                     GTAQELVYRGQPLATAVLLPEVPEQAPEGFTLIAPDDIATDGLRPLDGAISSINIIRV
                     LLWFVAAMIIGTMIYLSALERRRDVAVLKAVGSSTGQMAASIALQGALTALAAALIAA
                     VLQVALVPVFPLEVTVPARAFWQIPVIAVLVSLLAGAVGLRKAVSTDPALAFAGPGS"
     gene            2740..3744
                     /locus_tag="VLR27_09545"
     CDS             2740..3744
                     /locus_tag="VLR27_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSP03733.1"
                     /translation="MSASSVEVRDLVIEYASGGYSVRPIDGLDLDAAPGELVVLLGPS
                     GCGKTSLLSVLGGILRPTSGSVRVGGVAVESLRGTALAEYRKQTVGFVFQAFNLIPSL
                     SARENVAMPLLMGGVKRSRALERADRLLDRVGLADRAKHKPSKLSGGQQQRVAVARGL
                     AADPAVLLADEPTANLDYIQAEGIISLLRDLRADGRSIVVSSHDDRLVPIADRVVHLV
                     PEFREDEQPPHLEEFSAGSVVFEQGARGELVYVIRSGTVEIIRQLADGGVEQLATLGP
                     DDYFGELGPLLGFPRSATARAVSDLQLMAYSVHDFREQVLGSARSSPNGHAQPVPVVP
                     "
     gene            complement(3793..>4735)
                     /locus_tag="VLR27_09550"
     CDS             complement(3793..>4735)
                     /locus_tag="VLR27_09550"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HSP03734.1"
                     /translation="VPEIIVGRAADGTRWVTTVGAAGELPEPEIVAEAGAAEPRPSRY
                     EVAGVQDVEAWMQTVADATKRIAAGDFDKVVLAREVRVTADVALPRLALLHRLRRAFP
                     GCFTFAVGPLVGASPELLVSRAGETVRAHPMAGTAPRGGDPTTDARLAATLLASSKDR
                     AEHQITIDMVWETLLPFSSYVDSEPEPSIVAVANVQHLATMVEGRLSHPPASVLELVA
                     ALHPTPAVAGWPVDPALAYIAAEEGIDRDRYAGPVGWVDAAGNGTWAVGIRSAIVDGP
                     VARLYAGVGVVADSDPQAELAETEAKLQAVLSAIVRP"
BASE COUNT          619 a         1786 c         1635 g          695 t
ORIGIN      
        1 caccggccgc ctgcgagcat tccgtcgcgg ccccgacggg gtcgaggtcg aggtcggcga
       61 ggtcggccgg gcggagatgg ccggcgagat ggcgctgctc gaggacctgc cccgtggcgc
      121 caccgtctac gccgtgcgcg acagccacct cgtgcggttc tcccgggcgg ccttcagcgc
      181 ggtcatcacg cgctacccgt cggcagggct catggtggcc cagaccgtcc tgcgccgcgc
      241 gtccaagcgg gacgaccgtc cgcagcgcct gtcggtggcg gtcatcggcg gtgccggtga
      301 agtggacgtc gacgggttcg cccgccagct ggctgagtcg ctcggttccg aagcccgcta
      361 cgtgaccagc gccgacatcg atgccgagct cggcgtggac ggactcgcgc aggtcgagga
      421 cgatgaggtg ggcagcgtgc ggctggccta ctggctggag gagctccagg agcgcaaccg
      481 cttcatcgtg tacggggtcg acgagacctg gtccacctgg agccggcgtg cgctccgctg
      541 gtccgaccac gtcctcgtcg tggcggatgc ggaggccgat cccaagccga cgccgttcga
      601 ggccgagatg tggcggctgg tcgagcagca gaagcacccc aaggtgagcc tggccctgct
      661 gcaccccgcc gacaccgagc tgccctcggg caccccacgc tggttggaac cacggcacct
      721 ctcgtcgcac caccacgtgc gcatggggga ccaggtcacc gtcgatcgcc tggcccgcct
      781 cctcgccggt cgcggcacgt cgctcgtgct cggcggcggc ggagctcgcg ggttcgccca
      841 cctcggcgtg ctcgtcgtgc tcgacggcac cggggtgccg atcgacatgg tcggcggcac
      901 cagcatcggg tcgatcatgg ctgccggtcc cgccatgggc tggagcgccg agaagtcccg
      961 gatgctcgcc atggagcagt tccgcaagat cttcgactac accctgccgg ccgtggcggt
     1021 gctcaagggt gagcgcgtca ccaccaagct gcgcaaggcc ttcggggatg tcgacatctc
     1081 cgacctgtgg atcccgtact tctgcgtgtc caccaacctc accaccgccg gggcccacta
     1141 ccacgaccgc ggaccgctcg tggacgccat ccgcgcgagc gtcgccctcc ccggcgtgct
     1201 gccacccgtg cccttcgagg gcgagctgct cgtcgacggc gggatcctcg acaacgtccc
     1261 ggtcgatgag atgcgtcgcc ggaacacgac cggacgcgtc atcgccatcg acgtcgcccc
     1321 ggccgagggt ccctccgtga cccacgacta cggcctctcc atgtccggct tccgcgccct
     1381 gctcgaccgc cgccggggcg atggcccgcc ggcgatgctc gccacgctcg tgcgctccag
     1441 cctcatcgcc tccgtccgcg accgcgaccg ggtggtgcgc gaccgcgtcg ccgacctgta
     1501 cctcgacgtc accgtcgacg gcgccggact cctcgacttc gacgccgccg agcacatcgc
     1561 ggcgaacgcc gagtccatca cccgcccgat catccgccgc tgggtgcaaa gcaccgatcc
     1621 caaggagctc agctatgtcc agaccaccgc atcagcgggg accgtcctct cggacgtgtc
     1681 ggcccgggcg agggtggccg gcatgatcct gctgaccctc cgcgacctgc agcaccgcgc
     1741 cgtccgcttc gccgccgtca tcgccggcac cgccgtggtg ctcgccctgc tgttcctcat
     1801 gaccgggctg atcgagcagt tccacctcga acccgaggac accgtcgacg cactcggtgc
     1861 cgagtcgtgg ctgctccgcg acggcgtgtc cggcgcgttc accgccgcat cgacgatgcc
     1921 ggccgacacc gccgacgccc tcggcgacgg ggccgcgccg gtcgtggtcg cccgccacag
     1981 catcgccggt gcgagcgagc agaccgacgt cgtcatcatc ggctaccagc agggtgagct
     2041 gggtgagccg ccgctcgacg atggcgcgct gccggccaac gaccgcgagg tcctgatcga
     2101 cgactctgcc ggcgtctcgc cgggcgagga gatcgccctc ggcggcgagc agtacctggt
     2161 gtcgggcacc acggaccgga cgacgatgtt cgccggcatg ccgctggtgt tcatggacat
     2221 cggcaccgcc caggagctcg tctaccgagg ccagccgctg gccaccgccg tcctgctgcc
     2281 cgaggtgccg gagcaggccc cggaggggtt cacgctcatc gcccccgacg acatcgccac
     2341 cgacggcctc cgtcccctcg atggtgccat ctcctcgatc aacatcatcc gggtcctgct
     2401 ctggttcgtc gccgccatga tcatcggcac gatgatctac ctgtcggcgc tggagcgccg
     2461 ccgcgacgtc gccgtcctca aggccgtcgg cagctccacc ggccagatgg ccgcatcgat
     2521 cgccctccag ggcgcgctca ccgccctggc cgccgcgctc atcgctgcgg tcctgcaggt
     2581 cgctctcgtc cccgtcttcc cactggaggt caccgtgccc gcccgagcct tctggcagat
     2641 accggtcatc gccgtcctgg tgtcgctcct cgccggagcg gtcggcttgc gcaaggccgt
     2701 cagcaccgat cccgccctcg ccttcgcggg tccgggctca tgagcgcctc cagcgtcgag
     2761 gtccgcgacc tcgtcatcga gtacgccagc ggcggctact ccgtccgtcc catcgacggg
     2821 ctcgacctcg acgccgcccc tggggagctg gtcgtgctgc tcggcccgtc cggttgcggc
     2881 aagacgtcgc tgctgtccgt gctgggcggc atcctccgac cgacatccgg atcggtgcgg
     2941 gtcggtggcg tcgccgtcga gtcgctgcgc ggcactgcgc tcgcggagta ccgcaagcag
     3001 acggtcggct tcgtcttcca ggccttcaac ctgatcccgt ccctgagcgc ccgcgagaac
     3061 gtggcgatgc cgctgctcat gggcggcgtg aagcggtccc gcgccctcga gcgcgccgac
     3121 cggctcctcg accgggtcgg cctggccgac cgcgccaagc acaagccctc caagctctcg
     3181 ggcggccagc agcagcgggt cgcggtggca cgtggcctgg ctgccgaccc ggcggtcctg
     3241 ctcgccgacg agcccaccgc caacctcgac tacatccagg ccgagggcat catctcgctg
     3301 ctccgcgacc tgcgtgccga cggccgctcg atcgtggtgt ccagccacga cgaccgcctc
     3361 gtgccgatcg ccgaccgggt cgtgcacctg gtgccggagt tccgcgagga cgagcagccg
     3421 ccccacctcg aggagttctc ggccgggtcg gtcgtgttcg agcagggcgc tcgaggcgag
     3481 ctcgtctacg tcatccggtc gggcacggtg gagatcatcc gccagctcgc cgacggcggc
     3541 gtcgagcagc tggcgacgct cggcccggac gactacttcg gcgagctcgg cccgctgctc
     3601 gggttccccc gatcggcgac ggcccgggcc gtctcggacc tgcagctcat ggcctacagc
     3661 gtccacgact tccgcgagca ggtgctcggc agcgcccgat cgagccccaa cggccatgcg
     3721 caaccggtgc ccgtcgtccc ctgaccgttc tggcagcgac tgcgggacgc gttccgtggc
     3781 cgctgccggt tctcagggac ggacgatggc ggacaggacc gcctggagct tggcttcggt
     3841 ctcggccagc tcggcctgcg ggtcggagtc agccaccacg cccacgccgg cgtagagccg
     3901 ggccacggga ccgtcgacga tggccgagcg gatgccgacg gcccacgtgc cgttgccggc
     3961 ggcgtcgacc cagccgaccg gtccggcgta gcgatcccgg tcgatgccct cttcggcagc
     4021 gatgtaggcc agcgcgggat cgaccggcca gcccgccacg gccggggtcg ggtgcagcgc
     4081 ggcgacgagc tccagcaccg atgccggggg gtgcgagagg cggccctcga ccatcgtcgc
     4141 caggtgctgc acgttggcca ccgccacgat cgacggctcg ggctccgagt cgacgtacga
     4201 cgagaagggc agcagcgtct cccacaccat gtcgatggtg atctggtgct cggcccggtc
     4261 cttgctggag gcgagcagcg tcgcggccag gcgggcgtcg gtggtggggt cgccgccgcg
     4321 gggcgcggtg ccggccatgg ggtgggcgcg gacggtctcg ccggcccggg acaccagcag
     4381 ctcgggcgac gcgccgacca gcggaccgac ggcgaaggtg aagcagccgg ggaacgcccg
     4441 tcggaggcgg tgcagcaggg cgagccgggg gagggcgacg tcggccgtga cccggacctc
     4501 ccgggccagc acgaccttgt cgaagtcgcc ggcggcgatg cgcttggtgg cgtcggcgac
     4561 ggtctgcatc caggcttcga cgtcctgcac gccggcgacc tcgtagcgcg acggccgcgg
     4621 ctcggcggcc cccgcctcgg ccacgatctc cggttctggc agttcccctg cagcgccaac
     4681 ggtcgtgacc catcgggtgc cgtcggcggc tcggcccacg atgatctcgg gcacg
//
LOCUS       DATISU010000236         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_292220, whole genome shotgun sequence.
ACCESSION   DATISU010000236 DATISU010000000
VERSION     DATISU010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292220"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VLR27_09555"
     CDS             complement(<1..790)
                     /locus_tag="VLR27_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HSP03735.1"
                     /translation="MPAERPTEPMRARFDRIRARTEEATEAPRATLRFGVYAVVGMVV
                     GLAVAAIQWIALDVVLHELLQAPLWFQALAPGLGLAVTFVVLRGRTTPATSDDYVRVY
                     HDGDDLRPRQLVPKLLGAVATLGGGGALGAEGPSVYAGARIGQRLGLRLTSVLGPRGH
                     RALTSAGAAAGVAAIFQAPATGVLFALETPFQRDVARRALIPALIAAATSYLTFVSIF
                     GVHSYLPVPRGEVRLRDEVFGAILLGLCAGITARGLAGLWGAAKG"
     gene            852..1931
                     /locus_tag="VLR27_09560"
     CDS             852..1931
                     /locus_tag="VLR27_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03857 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HSP03736.1"
                     /translation="MTAANAQPHPELGFYALAGQPESSRDLLQEVRDAEALGLGTAFI
                     SERYDLKEAATLCGAAGAVSERIHIQTATTNHNTRHPMVTAGIARTMQDLTGGRFVLG
                     IGRGIPAMQDAFGMSRITTAQMEDFVGIMRRLFRGEVVVGHDGPAGSWPVLHLNRTLD
                     EHLPMGLVAFGPESLKLGGRCFDEVVLHTFFTDETTERAVRTVKESAEQAGRDPDDVV
                     VWSCFATIGDHLPEDLRLKKSVGRLATYLQGYGDLMVRTNGWDPAVLERFRADPLVAG
                     IGGPIDGVATTEQLEHIAALLPDDWLAPSATGTPEQCASAVRGQLALGCDAVILHGAT
                     PTQLQPVVESYRSGSGSGGGTPALR"
     gene            complement(2008..>2318)
                     /locus_tag="VLR27_09565"
     CDS             complement(2008..>2318)
                     /locus_tag="VLR27_09565"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HSP03737.1"
                     /translation="ERHPDLLTVEFAKDDRGDRLFVDVLRNAPAQHAVAPYTLRPKPG
                     APGAVPLRWDEVDGSWHPQSTPLTSLFRRLGALARDPWEGIDAARLPAADIAERVQRL
                     "
BASE COUNT          324 a          840 c          871 g          283 t
ORIGIN      
        1 ggcccttggc cgccccccac agaccggcca gcccgcgggc cgtgatgccg gcgcacaggc
       61 cgagcaggat cgccccgaag acctcgtcgc gcaggcgaac ctcgccccgg ggcaccggca
      121 ggtacgagtg cacgccgaag atcgacacga aggtgaggta cgaggtggcc gcggcgatca
      181 gggccgggat gagcgcgcgc cgggccacgt cgcgctggaa gggcgtctcg agtgcgaaga
      241 gcacgccggt ggcgggcgcc tggaagatgg ccgcgacgcc ggcggccgcg ccggccgagg
      301 tcagggcgcg gtggccccgg ggcccgagca ccgacgtgag ccgcaggccg aggcgctggc
      361 cgatgcgggc accggcgtag accgagggac cctcggcgcc gagggcgccg cccccgccca
      421 gggtggcgac ggcgccgagc agcttgggca ccagctgtcg cggccggagg tcgtcgccgt
      481 cgtggtacac ccgcacgtag tcgtccgacg tggccggggt cgtgcgtcct cgcagcacca
      541 cgaacgtgac ggccaggccg aggccgggcg cgagggcctg gaaccacagg ggggcttgga
      601 gcagctcgtg gagcacgacg tcgagcgcga tccactggat cgccgccacc gcgaggccga
      661 cgaccatgcc gacgacggcg tagacgccga agcggagcgt ggcccgaggt gcctcggtgg
      721 cctcctcggt ccgggcccgg atccggtcga accgggcgcg catcggttcg gtcggtcgct
      781 cggcgggcac ggggcggaat ctacggtgac gggtccgacc ccgccgacct cggcccgacg
      841 acggagcgcg catgaccgcc gcgaacgccc aaccgcaccc ggagctgggg ttctacgccc
      901 tggccggcca gcccgagtcg agccgcgacc tgctccagga ggtgcgcgac gccgaggcgc
      961 tcgggctcgg gaccgcgttc atctccgagc gctacgacct caaggaggcc gcgaccctct
     1021 gcggggcggc cggtgcggtg agcgagcgga tccacatcca gacggcgacg acgaaccaca
     1081 acacccgtca ccccatggtc accgccggca tcgcccgcac catgcaggac ctcaccggcg
     1141 gtcggttcgt gctcggcatc gggcggggca tcccggccat gcaggacgcg ttcggcatgt
     1201 cgcgcatcac gaccgcgcag atggaggact tcgtcgggat catgcgccgg ctgttccggg
     1261 gggaggtggt ggtgggccac gacggccccg ccggctcgtg gccggtcctg cacctgaacc
     1321 gcaccctcga cgagcacctg ccgatgggcc tggtggcgtt cggccccgag tcgctgaagc
     1381 tgggtgggcg ctgcttcgac gaggtcgtgc tgcacacgtt cttcaccgac gagaccaccg
     1441 agcgcgccgt gcgcacggtg aaggagtccg ccgagcaggc cggtcgcgat cccgacgacg
     1501 tcgtcgtgtg gtcctgcttc gccacgatcg gcgaccacct cccggaggat ctgcggctga
     1561 agaagtcggt cgggcgcctg gccacctacc tgcagggcta cggcgacttg atggtccgca
     1621 ccaacggctg ggatcccgcg gtgctcgagc ggttccgggc cgaccccctg gtcgctggca
     1681 tcggcggccc gatcgacggc gtcgccacca ccgagcagct cgagcacatc gccgcgctcc
     1741 tgcccgacga ctggctggcg ccgagcgcca ccggcacccc ggagcagtgc gcgtcggcgg
     1801 tgcggggcca gctcgccctc ggctgcgacg ccgtcatcct ccacggcgcc acccccaccc
     1861 agctccagcc ggtggtcgag tcctaccgct ccggttctgg cagcggaggc ggaacaccag
     1921 cactgcggtg acgtccccgt ctggtagccc agtgttccgc agccgctgcc agaacccatc
     1981 gggcgttccg tagccgctgc cagaacgtca gagccgttgc actcgttccg cgatgtcggc
     2041 ggcggggagc cgggcggcgt cgatgccctc ccacgggtcg cgggccaggg caccgaggcg
     2101 ccggaacagc gaggtgagcg gtgtgctctg cgggtgccag ctgccgtcga cctcgtccca
     2161 gcgcagcggc acggcccccg gcgcgcccgg cttgggtcgc agcgtgtagg gcgccacggc
     2221 gtgctgggcg ggggcgttgc ggagcacgtc gacgaagagc cgatcgccgc ggtcgtcctt
     2281 ggcgaactcc accgtgagca ggtcggggtg gcgctcgg
//
LOCUS       DATISU010000237         4576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_571649, whole genome shotgun sequence.
ACCESSION   DATISU010000237 DATISU010000000
VERSION     DATISU010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571649"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..625
                     /locus_tag="VLR27_09570"
     CDS             122..625
                     /locus_tag="VLR27_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03738.1"
                     /translation="MSPVAKRRGRGHTGRAVAVAVVGVAVALGTAFLVANLASRGDVQ
                     VRLGDDRFDAGRVENLARIIEEDGQPILVPDPANFSRSIYVDHQGGDPTTGWIALSAF
                     VPDQPECTLTFDPEVELFTIDDAQPASCDRGTTFPRSGAGLRVYATEVVDGTLTIDLQ
                     DQANAPD"
     gene            complement(585..2342)
                     /gene="treZ"
                     /locus_tag="VLR27_09575"
     CDS             complement(585..2342)
                     /gene="treZ"
                     /locus_tag="VLR27_09575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HSP03739.1"
                     /translation="MWAPDASEVALHVDGRDVPATPADGGWWVAHAEAGHGTRYGWRL
                     DGADPLPDPRSRWQPDGVHGLSAVDAPDETDFSWTDQHWRGFHLPGAVLYELHVGTFT
                     PEGTFDGVVERLPHLVDLGVDAIELLPVAAFPGDRGWGYDGTFPHAVHDAYGGPAGLR
                     RLVDAAHAAGIGVVLDVVHNHLGPDGNHLGAYGPYFSERHQTNWGAAVNLDGPGSDEV
                     RRYFVEHLTGWQRDLHLDGFRLDAVHALVDDSATHLLEQLADEVAAAVAHTGRSCWLI
                     AESDRNDPRYVLPAAAGGMGLDASWADEWHHALHAVLTGETDGYYEDFGTLDHLVRGL
                     QQAWVYAGDWSQHRQRVHGRSPDGLPGHTFVVSVQNHDQIGNRATGDRIGHGISEGRQ
                     RIAAAFLLTSPFVPMLFQGEEWSASSPFRYFTDHQDAELGRAVTEGRTHEFAHFGWDP
                     ADVPDPQDPATFESSVLRWDEVGEPGHAELLQWHRDLIALRRRHPSLTDGRWAEQAID
                     HDADVGWIRIRRGDVLVVVNLGSAPVSVPVPDGFALAEVEGRPLAAGATRNADVVALD
                     PDAVAVLSRGRSPGPAGRW"
     gene            complement(2354..>4576)
                     /gene="treY"
                     /locus_tag="VLR27_09580"
     CDS             complement(2354..>4576)
                     /gene="treY"
                     /locus_tag="VLR27_09580"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HSP03740.1"
                     /translation="VRFRAEAPGHRFPLAPRSLPLLLHPAAARLADDADVDPLAADSL
                     AFVADALDALEPATTDRARRRRAGHLDVVQHLLQRLLDQPAVAAAVDAEIAAVNADPD
                     ALHGLLEEQHYRLAYWRTGARELDYRRFFDITTLVAIRVERDSVFDAVHELPLRWLAE
                     GRIDGLRIDHPDGLADPTGYVQRLRAVAPDAWIVVEKIVEGDEWLPEDWPVDGTTGYE
                     AMRLLNGVLVDPAGLDVLAAVERRAHELCGTEAPQPEAVVLESKRHAVSELLAAELER
                     VVDLALRVAETRPLHRDHPRDDLRRAIAGLAVGFDVYRSYVVPGVDGGASTVSDTDVA
                     RIDHAAAEAKAGTDVDPRLIDFLADVLTLRAVHPEDGPTDDERELVIRFQQLTGPATA
                     KGLEDTAWYRLGRFIAAAEVGGDPLHPAVSVEHLHEACRHRQERWPHTMTTLSTHDTK
                     RSADVRARLDVLSEPTLEPSWPQLADRWLPRLLQRWPADVEPDATTLLVALQTLLGAW
                     PISSDRLEAYLVKAAREAKRRTSWTEVDDGFESGLAALASIAEDQAVADELAADVAVL
                     ERHGWWNSQVQTAIGLLQPGIPDVYQGTEIIDLSLVDPDNRRPVAHDVIAGELDDLDV
                     LRAPALGPIAKLALTTAGLLLRRRRPDAFGAGEAGAHQPLTASGPDADRVVAFTRGDQ
                     VAVVAVRFPSRGPVAAGTTVELPVGEWSPVFGGNAVAGGTVDLADLLGDTPVAVLELR
                     "
BASE COUNT          592 a         1576 c         1825 g          583 t
ORIGIN      
        1 ggcgaagaac tgcttgccct ggtattcgag gaggtccatg cgtgcttccc ttgtctggct
       61 tgggccgggg cggccttcgg ccggatacta ggaggatgcc caccagcccc ggccaatccc
      121 gatgagcccg gtcgccaagc gtcgcggccg cggccacacc ggccgggccg tcgccgtcgc
      181 cgtggtcggc gtcgcggtcg cgctgggcac cgccttcctg gtggccaacc tggccagccg
      241 gggcgacgtg caggtccgcc tcggcgacga ccgcttcgac gccggccggg tcgagaacct
      301 cgcccgcatc atcgaggagg acggccagcc gatcctggtc ccggatccgg ccaacttctc
      361 ccgctcgatc tacgtcgacc accagggcgg cgacccgacg acgggctgga tcgcgctcag
      421 cgccttcgtg cccgaccagc cggagtgcac gctcacgttc gaccccgagg tcgagctgtt
      481 caccatcgac gacgcccagc cggcctcgtg cgaccggggc accaccttcc cccggtccgg
      541 tgcgggcctg cgggtctacg ccaccgaggt cgtcgacggc accctcacca tcgacctgca
      601 ggaccaggcg aacgcccccg actgaggacg gcgacggcgt cggggtcgag ggcgaccacg
      661 tcggcgttgc gggtggcccc ggcggcgagc gggcggccct ccacctcggc cagggcgaag
      721 ccatcgggga ccggcaccga gacgggggcc gagccgaggt tcacgacgac gagcacgtcg
      781 ccccggcgga tgcggatcca gccgacgtcg gcgtcgtggt cgatcgcctg ctcggcccag
      841 cggccgtcgg tgagcgatgg gtggcggcgg cgcagggcga tgagatcgcg gtgccactgg
      901 aggagctcgg cgtggcccgg ttcgccgacc tcgtcccagc gcagcaccga ggactcgaac
      961 gtggccggat cctgggggtc ggggacgtcg gcggggtccc agccgaagtg ggcgaactcg
     1021 tgggtgcgcc cctcggtgac ggcccggccg agctcggcgt cctggtggtc ggtgaagtag
     1081 cggaacggtg acgaggccga ccactcctcg ccctggaaca gcatcgggac gaacgggctg
     1141 gtgaggagga aggcggcggc gatgcgctgc cggccctcgg agatgccgtg gccgatgcga
     1201 tcgccggtgg cccggttgcc gatctggtcg tggttctgca ccgagacgac gaaggtgtgg
     1261 ccgggcaggc cgtcgggcga gcggccgtgc acccgctggc ggtgctggga ccagtcgccg
     1321 gcgtagaccc aggcctgctg caggccccgc accaggtggt cgagcgtgcc gaaatcctcg
     1381 tagtagccgt cggtctcgcc ggtgagcacg gcgtgcaggg cgtggtgcca ctcgtcggcc
     1441 caggacgcgt cgaggcccat gccaccggcc gccgccggca gcacgtagcg cgggtcgttg
     1501 cggtcgctct cggcgatgag ccagcacgac cgaccggtgt gggcgacggc ggcggcgacc
     1561 tcgtcggcga gctgctcgag gaggtgggtg gcggagtcgt cgaccagcgc gtgcacggcg
     1621 tcgaggcgga agccgtcgag gtgcaggtcg cgctgccagc cggtgaggtg ctcgacgaag
     1681 taccggcgca cctcgtcgct gcccgggccg tcgaggttca cggcggcacc ccagttcgtc
     1741 tggtggcgct cggagaagta gggcccgtag gcgccgaggt ggttgccgtc ggggccgagg
     1801 tggttgtgga ccacgtcgag gacgacgccg atgccggcgg cgtgggcggc gtcgaccagg
     1861 cggcgcaacc cggccgggcc gccgtaggcg tcgtgcacgg cgtgggggaa cgtgccgtcg
     1921 tagccccagc cccggtcacc ggggaacgcc gccaccggca ggagctcgat ggcatcgacg
     1981 ccgaggtcca ccaggtgggg cagccgctcg acgacgccgt cgaaggtgcc ctccggggtg
     2041 aaggtgccga cgtggagctc gtagagcacg gcgccgggga ggtggaagcc ccgccagtgc
     2101 tggtcggtcc acgagaagtc ggtctcgtcg ggggcgtcga cggcggagag gccgtggacg
     2161 ccatcgggct gccaccgcga gcggggatcc ggcaacggat cggcaccgtc gaggcgccag
     2221 ccgtagcggg tcccgtggcc ggcctcggcg tgggccaccc accagccgcc gtccgccggc
     2281 gtggccggga cgtcccgccc gtcgacgtgc agcgcgacct cggacgcgtc cggcgcccac
     2341 atcgcgaacc gcctcatcgg agctccagga cggcgacggg cgtgtcgccg aggaggtcgg
     2401 cgaggtcgac ggtgccgccg gcgacggcat tgccaccgaa gaccggtgac cactccccca
     2461 ccggcagctc gacggtggtg ccggcggcga ccgggccgcg gctggggaag cggacggcga
     2521 cgacggccac ctggtcgccg cgggtgaagg cgacgacccg gtcggcgtcg gggcccgacg
     2581 cggtcagggg ctggtgagcg ccggcctcgc ccgcgccgaa ggcatccggt cggcggcgcc
     2641 gcagcagcag gccggcggtg gtcagggcca gcttggcgat ggggccgagg gccggcgcac
     2701 gcaggacgtc gaggtcgtcc agctcgccgg cgatgacgtc gtgggcgacg gggcggcggt
     2761 tgtcggggtc gaccagggac aggtcgatga tctcggtgcc ctggtacacg tcggggatgc
     2821 cgggctgcag caggccgatg gcggtctgca cctggctgtt ccaccacccg tggcgctcga
     2881 ggacggcgac gtccgcagcg agctcgtcgg ccacggcctg gtcctcggcg atcgaggcga
     2941 gcgcagccag gccggactcg aagccgtcgt cgacctcggt ccacgacgtg cggcgcttcg
     3001 cctcgcgggc ggccttcacc aggtacgcct cgaggcggtc gctgctgatc ggccacgcgc
     3061 cgagcagggt ctgcagggcc acgaggaggg tggtggcgtc gggttcgacg tcggccggcc
     3121 agcgctggag caggcggggc agccagcggt cggccagctg cggccacgac ggctcgaggg
     3181 tgggctcgct gagcacgtcg aggcgggcgc gcacgtcggc gctgcgcttg gtgtcgtggg
     3241 tcgacagggt cgtcatggtg tggggccagc gctcctggcg gtggcggcag gcctcgtgga
     3301 ggtgctcgac cgagaccgcg gggtgcaggg ggtcgccgcc gacctcggcc gcggcgatga
     3361 agcgaccgag ccggtaccag gcggtgtcct ccaggccctt cgccgtggcc gggccggtga
     3421 gctgctggaa ccggatcacc agctcccgct cgtcgtcggt gggcccgtcc tcggggtgca
     3481 cggcacgcag cgtcagcacg tcagcgagga agtcgatcag gcgggggtcg acgtcggtgc
     3541 cggccttggc ctctgcggcg gcgtggtcga tgcgagcgac gtcggtgtcg ctcaccgtgc
     3601 tcgccccacc gtcgacgccg ggcaccacgt agctgcggta gacgtcgaac cccacggcga
     3661 ggccggcgat cgcccggcgg aggtcgtccc gggggtggtc ccggtgcagg gggcgggtct
     3721 cggcgacgcg cagggcgagg tcgaccaccc gctcgagctc ggcggcgagc agctccgaca
     3781 cggcgtggcg cttggattcg agcacgacgg cctcgggctg gggcgcctcg gtgccgcaca
     3841 gctcgtgggc gcgccgctcg accgcagcca ggacgtcgag cccggccggg tccacgagca
     3901 ccccgttgag caggcgcatg gcctcgtacc cggtggtgcc gtcgaccggc cagtcctcgg
     3961 gcagccactc gtcaccctcg acgatcttct ccaccacgat ccaggcgtcg ggtgccacgg
     4021 cccgcagtcg ctgcacgtac cccgtcgggt ccgcgagccc gtccgggtgg tcgatgcgca
     4081 ggccgtcgat gcggccctcg gcgagccagc gcaacggcag ctcgtggacg gcgtcgaaga
     4141 ccgagtcgcg ctcgacccgg atggcgacga gcgtggtgat gtcgaagaac cgccggtagt
     4201 ccagctcgcg ggctccggtc cgccagtagg cgagccggta gtgctgctcc tcgaggaggc
     4261 cgtggagcgc gtcggggtcg gcgttcaccg cggcgatctc ggcatcgacc gccgccgcga
     4321 ccgcaggctg gtcgagcagc cgctgcagca ggtgctgcac gacgtcgagg tggccggcac
     4381 ggcgacggcg cgcccggtcg gtggtggccg gctccagcgc gtcgagggcg tccgccacga
     4441 aggcgagcga gtcggcagcc agcgggtcga cgtcagcgtc gtcggccagg cgcgccgccg
     4501 ccgggtgcag cagcagcggc aggctccggg gtgccagcgg gaagcggtgg cccggcgctt
     4561 cggcacggaa gcggac
//
LOCUS       DATISU010000238         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_152516, whole genome shotgun sequence.
ACCESSION   DATISU010000238 DATISU010000000
VERSION     DATISU010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152516"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VLR27_09585"
     CDS             <1..1116
                     /locus_tag="VLR27_09585"
                     /inference="COORDINATES: protein motif:HMM:NF037683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03741.1"
                     /translation="VPLIELLDVVGHDVVTHHPLQPFDPEAFADDAPWSFDPVQLAGA
                     RAVVGARVPPPPFLSGSLEDRAEAVVGLDELVRFVQDPLRELLRQRLGMSTWDDDDQL
                     PDAIPIELDGLQRWGVGQRILRQLLAGIDLDDAVRAEIASGTLPPGELRHELLTDVAN
                     TAGAIHRLAVDEGVAGPPTSVEVDVDADLFEAGGDPVRVLGTVPGVHGDTVAEVSFSS
                     LSPKTRLALWVRLLAVSAARPEREWCTLGIGRTRNGGKVVRHRALDGPPEHRSAEAAR
                     LLGRLVALRQRGLREPLPLPCKTAEAWASAAHREQDTRDAAGRCWSGGRFDGDADDAS
                     AVRIYGEGATLDQLLAAGLDPLARHLWDPILVRESWR"
     gene            1113..>2119
                     /locus_tag="VLR27_09590"
     CDS             1113..>2119
                     /locus_tag="VLR27_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSP03742.1"
                     /translation="MITVLEASAGTGKTHRVTTEAVRAVAEGTPLEQMLLVTFTIAAT
                     SELRARTWDRLSRTAAALDAHLQGAPVETDDELVAELCAGDAATVGLRHRNLTDAMAS
                     FDGATITTIHGFCHQVLQSLGFAADVERDPTFVEDVRDMLDEVVADLLVRKLHRATGV
                     RFPVDPARRIAEKAIIDPRARIVPAPDGSSDDLRSRFAGAVRDEMDRRKRAAGLLGYD
                     DLLLRVEEALVEDPDGTVRDRLRRRYSLVIVDEFQDTDAVQWEILERAFGDPPTRLVL
                     VGDPKQAIYAFRGADVHAYLAARRRAGDEHVHHLDRSWRADAGVLRGLDALFGDTALG
                     H"
BASE COUNT          259 a          851 c          740 g          269 t
ORIGIN      
        1 gtcccgttga tcgagctgct cgacgtggtc ggccacgacg tcgtcaccca ccacccgctc
       61 cagcccttcg accccgaggc cttcgccgac gacgcgccgt ggagcttcga tccggtgcag
      121 ctggccggcg cccgggccgt cgtgggtgcg cgcgtgcctc cgccgccgtt cctgtccggt
      181 tccctcgagg accgggcgga ggccgtcgtc ggcctcgacg agctggtccg cttcgtccag
      241 gacccgctgc gcgagctgct ccgccagcgc ctcgggatgt cgacgtggga cgacgacgac
      301 cagctgcccg acgccatccc gatcgagctc gacggcctcc agcggtgggg cgtgggccag
      361 cgcatcctgc gccagctgct cgctggcatc gacctcgacg acgcggtgcg ggccgagatc
      421 gcgtcgggca cccttccgcc cggcgagctc cgccacgagc tgctcaccga cgtcgcgaac
      481 accgccggcg ccatccaccg cctcgccgtg gacgagggcg tggccggccc gcccacgtcg
      541 gtggaggtcg acgtcgacgc cgacctgttc gaggccggcg gcgacccggt gcgcgtgctc
      601 ggcaccgtcc ccggcgtcca cggcgacacg gtcgccgagg tgtcgttctc ctcgctcagc
      661 cccaagaccc ggctggccct gtgggtgcgg ctgctggcgg tgagcgcggc tcgccccgag
      721 cgggagtggt gcacgctcgg catcggccgg acccgcaacg gcggcaaggt ggtccgccac
      781 cgcgccctcg acggtccgcc cgagcaccgg tccgccgagg ccgctcggct cctcggacgc
      841 ctggtcgccc tgcgccagcg cgggctgcgc gagcccctgc cccttccctg caagaccgcc
      901 gaggcctggg ccagcgccgc gcatcgcgag caggacaccc gcgacgcggc cggccggtgc
      961 tggtcgggcg ggaggttcga cggggacgcc gacgacgcca gcgcggtgcg catctacggc
     1021 gagggcgcga ccctggacca gctgctcgcc gccgggctcg acccgctcgc ccgccacctg
     1081 tgggatccga tcctggtgcg ggagtcgtgg cgatgatcac cgtcctcgag gccagcgccg
     1141 gcacgggcaa gacccaccgg gtcaccaccg aggccgtgcg cgccgtggcg gagggcacgc
     1201 ccctcgagca gatgctgctg gtgaccttca ccatcgctgc caccagcgag ctgcgcgccc
     1261 gcacctggga ccggctctcc cgcaccgccg ctgcgctcga cgcccacctg cagggtgcac
     1321 cggtcgagac cgacgacgag ctggtcgccg agctgtgcgc cggggacgcg gcgaccgtcg
     1381 ggctccggca ccgcaacctc accgacgcca tggcctcctt cgacggggcc accatcacca
     1441 cgatccacgg cttctgccac caggtgctgc agagcctggg cttcgccgcc gacgtcgagc
     1501 gcgacccgac cttcgtcgag gacgtgcggg acatgctcga cgaggtcgtc gccgacctcc
     1561 tcgtccgcaa gctccaccgg gccaccggcg tgcgcttccc ggtcgacccg gcgcgacgga
     1621 tcgcggagaa ggccatcatc gatccccggg cccgcatcgt gcccgcgccc gacggcagct
     1681 ccgacgacct gcggtcccgc ttcgccggcg ccgtgcgcga cgagatggac cgccgcaagc
     1741 gcgccgccgg cctgctcggc tacgacgacc tcctgctccg ggtggaggag gcgctggtcg
     1801 aggaccccga cggcaccgtg cgcgaccggc tccggcgccg ctactccctc gtcatcgtcg
     1861 acgagttcca ggacaccgac gccgtgcagt gggagatcct cgagcgggcc ttcggcgacc
     1921 cgccgacccg cctcgtgctg gtcggcgacc cgaagcaggc catctacgcc ttccggggcg
     1981 ccgacgtgca cgcctacctg gccgcccgcc gtcgggccgg cgacgagcac gtccaccacc
     2041 tcgaccggag ctggcgggcc gacgccgggg tgctgcgggg cctcgacgcg ctgttcggcg
     2101 acaccgccct cggccacga
//
LOCUS       DATISU010000239         1882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_757672, whole genome shotgun sequence.
ACCESSION   DATISU010000239 DATISU010000000
VERSION     DATISU010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757672"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VLR27_09595"
     CDS             <1..333
                     /locus_tag="VLR27_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03743.1"
                     /translation="FAGIDIDLFAVGDARVKRLVYGVGGKWSEHVKPHVGTDWCEHAH
                     VGFLVKGRLAGEYPDGCAFDFTAPIGVHIDPGHDSWVVGDEPAVLIEVDFGPDTVERL
                     GIPGTHSH"
     gene            complement(355..1359)
                     /locus_tag="VLR27_09600"
     CDS             complement(355..1359)
                     /locus_tag="VLR27_09600"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HSP03744.1"
                     /translation="MNARSFPTRWSLAAALLALVAGVVAMPVADAAPSANAGPKTMVA
                     LGDSFAAGPLIPPQVDERPWGCLRSSLNYAHQIASALSLELTDVTCSGASTKHLWETH
                     GVSPDAELVEEGVPVLAGDHPGNPPQLDALRPDTDVVTLQIGGNDMGFGGIATTCAEA
                     ALTLGSCKAAVEAERPFDRIAQTAPKLRAVLAEIHRRSPQADVHVLGYPGIFAMGETA
                     SCPAMGVGEEDARYLRSIQEALNGMIRSVADEAGHGYSDATGYVDVYAPSAGHTACDL
                     PAIRWVEPFVPVNAAAPVHPNLGGMTAIRDLLLDEVWPEGIPRRAALPGLPAPPSLPG
                     "
     gene            1418..>1882
                     /locus_tag="VLR27_09605"
     CDS             1418..>1882
                     /locus_tag="VLR27_09605"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HSP03745.1"
                     /translation="MPFETVTGYCWPPSVAPGEAVGLHLSSAGGRPVQVEIARIGGTR
                     DVVWSETVGADFHPTPDGADANGCDWPAAVAVDVGDDWRSGYHEVVLTIDVDGKERTT
                     HAFFVVRPSERSENTILLQLATNTYHAYNDFGGRNLYTGGTTVSLQRPMSPGY"
BASE COUNT          261 a          679 c          691 g          251 t
ORIGIN      
        1 ttcgccggca tcgacatcga cctgttcgca gtcggcgatg cccgcgtgaa gcgactcgtc
       61 tacggggtgg gcggcaagtg gtccgagcac gtgaagccgc acgtcggcac cgactggtgc
      121 gagcacgcgc acgtcggctt cctggtcaag ggacgcctcg ccggcgagta ccccgacggc
      181 tgcgcgttcg acttcaccgc ccccatcggc gtccacatcg atcccggcca cgacagctgg
      241 gtggtgggcg acgagccggc ggtgctcatc gaggtcgact tcggacccga caccgtcgag
      301 cgcctcggca tccccggcac gcactcgcac tgagcggacg accccggcgg tcacctatcc
      361 cggcagggag ggcggcgccg gcagcccggg cagcgccgcc cggcgaggga tgccctcggg
      421 ccagacctcg tcgagcagga ggtcgcggat cgccgtcatg ccgccgaggt tcgggtggac
      481 cggagcggcg gcgttgaccg gcacgaaggg ctccacccag cggatcgccg gcaggtcgca
      541 ggccgtgtga ccggcgctcg gcgcgtagac gtcgacgtag ccggtggcat cgctgtagcc
      601 gtgcccggcc tcgtccgcca ccgagcggat catgccgttg agcgcctcct ggatgctgcg
      661 caggtagcga gcgtcctcct cgccgacgcc catggccggg cagctcgcgg tctcccccat
      721 ggcgaagatg cccgggtagc cgaggacgtg gacgtcagcc tggggcgagc ggcggtggat
      781 ctccgccagc acggcccgga gcttcggcgc cgtctgcgcg atgcggtcga aggggcgctc
      841 ggcctccacc gccgccttgc aggatccgag ggtgagcgcg gcttcggcgc aggtggtggc
      901 gatgccaccg aagcccatgt cgttgccgcc gatctgcagc gtcacgacgt cggtgtcggg
      961 gcggagggcg tcgagctggg gcgggttgcc cgggtgatcg ccggcgagca cggggacgcc
     1021 ctcctcgacg agctcggcgt cgggcgacac gccgtgggtc tcccacaggt gcttggtgct
     1081 ggcgccgctg caggtcacgt cggtgagctc cagcgacagg gctgaggcga tctggtgggc
     1141 gtagttcagg ctcgatcgca ggcaacccca cgggcgctcg tcgacctgcg gcgggatgag
     1201 gggacccgcg gcgaaggagt cgcccagcgc caccatcgtc ttcgggccgg cgttcgcgct
     1261 gggggccgcg tccgcgaccg gcatggcgac gacgccggcg accagcgcga gcagcgcggc
     1321 cgcgagcgac caccgggtgg ggaaggatcg agcattcacg ggctggactt cggcggcagc
     1381 cgccacacca cctgccccgc gcctagcgtc gccggccatg ccgttcgaga ccgtcaccgg
     1441 gtactgctgg ccaccgagcg tcgcgccggg tgaggccgtg ggcctccacc tctcctccgc
     1501 cggcggccgg ccggtgcagg tcgagatcgc ccggatcggc ggcacccgcg acgtggtgtg
     1561 gtccgagacc gtcggcgccg acttccaccc cacccccgac ggtgccgacg ccaacggctg
     1621 cgactggccg gcggcggtcg ccgtggacgt cggcgacgac tggcgatccg gctaccacga
     1681 ggtcgtcctc accatcgacg tcgacggcaa ggagcgcacg acccacgcgt tcttcgtcgt
     1741 gcggccgagc gagcgcagcg agaacacgat cctgctgcag ctggccacga acacgtacca
     1801 cgcctacaac gacttcggcg gccgcaacct ctacaccggc ggcaccaccg tgtcgctgca
     1861 gcggcccatg tcgcccggct ac
//
LOCUS       DATISU010000240         4313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_757745, whole genome shotgun sequence.
ACCESSION   DATISU010000240 DATISU010000000
VERSION     DATISU010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757745"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VLR27_09610"
     CDS             <1..734
                     /locus_tag="VLR27_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HSP03746.1"
                     /translation="TNVILGIGDPEYAPEGSDLLMALLTAGQELAQLMDEVAASKRGG
                     DGTDLTSVLVNAELADDRLDHGDLASFFILLVVAGNETTRNAIAWGLHLLTQHPDQRA
                     IWEQDFEGVAGTAVEEIVRIASPVTYMRRTATEDTEVHGRQISAGDKLAMFYLAANRD
                     EDVFDDPHRFDVRRSPNNHVGFGGPGPHFCLGAHLARREITVMFRELFHRLPDIQASG
                     PPELLLSSFIHGVKHLPATFTPGGA"
     gene            752..1207
                     /locus_tag="VLR27_09615"
     CDS             752..1207
                     /locus_tag="VLR27_09615"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSP03747.1"
                     /translation="MADISVTREIAAPPERVWGLVSDMTRMGEWSPEATGGTWLKGAA
                     GPAAGARFKGTNEAGGKRWATTCEVVECEPGERFSFRVTGGPFKVATWSYDFEPTEAG
                     TRVTESWKDERHPVMAKIFGKVTGVTDRAEHNRTNMERTLEALERAATS"
     gene            1314..3371
                     /locus_tag="VLR27_09620"
     CDS             1314..3371
                     /locus_tag="VLR27_09620"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HSP03748.1"
                     /translation="MGTQDERAVLRAVFDRLVPADDWPSLPDAGLFPYLEELRAEPLT
                     RRDAEEIDAGVAALERMAQAEHGQGLSALSPTVVDALIADLDPDHREVLVRAAARAYY
                     GHPEGAGARMIGQPRVPPPVPGAEPALRTTDLGAVDADYDVVVLGAGAGGGVAACVLA
                     EAGARVLLVERGHAEVTGDPDDLRNHRTKAHGLGTRPAEGEPRVVEDGDGTHLVERPW
                     DPESHLGVRIVGGGTRVYQGLAWRFLPDDLRMASRYGVPDGSSLADWPLTYDDLASHY
                     DWVEWEVGVCGDPAGHRAAGHRQRGYPMPPLPEDPEAALLAAGADRLGLATGRVPMLL
                     NSVPRDGRAACGGADCGACVGFPCPTGGRNGTHDTVIPRALASGAVLITGTRAVEITA
                     GPDRVDGVQLVDERTGTARHIRAGHVVVACGAIETARLLLASRSDAHPGGLGNQTDQV
                     GRHLQGHAFASAFGSFDVDLDGPGPGATIATLDLAHGIADDDGRPLVGGGLVGNELRK
                     LAIVHWQWALPPGAPRWGAAGKAAMRDTFRRTGHLFAQVQEVPVPTNRVRLDPDVRDA
                     LGMPVARLSGAAHPETVRTARAVVGRAEEWLAASGATRTWVDDVPTGLLAGTHQAGTC
                     RMGTDPATSVTDPDGRVHGFGNLWVADASVHVTNGSVSPALTIMALAHRLATKLAAAA
                     SHR"
     gene            complement(3423..>4313)
                     /locus_tag="VLR27_09625"
     CDS             complement(3423..>4313)
                     /locus_tag="VLR27_09625"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HSP03749.1"
                     /translation="VADVEGNVVRSWGDPDRLTHPRSAVKPLQALPLIETGAADAFDL
                     GPVQLALACASHNGEPGHVAAVDVWLERIGCSADDLECGVEQAHRDHDPGATAARNNC
                     SGKHAGFLTVARHLGVDITGYIRPEHPVQELVRAALAETTRTALDPAEAGTDGCGIPV
                     HPMPLHAIATAAAGFGTPTEWPPERADASRRLADAMVAEPWFVAGTGRLCTDLLAAAN
                     GDVLVKTGAEGVQFAALPRLGLGIAIKAEDGSRPASEVALGHVLAEVDGTDRPEVFDS
                     RRRITNHVGTHVADLRIIPT"
BASE COUNT          568 a         1570 c         1621 g          554 t
ORIGIN      
        1 ggaccaacgt gatcctcggc atcggggacc cggagtacgc gccggagggg agcgacctgc
       61 tcatggccct cctcaccgcc gggcaggagc tggcccagtt gatggacgag gtggcggcct
      121 cgaagcgggg tggtgacggc accgacctca ccagcgtgct ggtcaacgcg gagctggccg
      181 acgaccggct cgaccacggc gacctggcca gcttcttcat cctcctcgtg gtggccggca
      241 acgagaccac ccgcaacgcc atcgcctggg ggctccacct gctgacccag cacccggacc
      301 agcgggcgat ctgggagcag gacttcgagg gcgtcgccgg taccgcggtc gaggagatcg
      361 tgcgcatcgc cagcccggtg acctacatgc ggcgcaccgc caccgaggac accgaggtcc
      421 acggtcgcca gatcagcgcc ggcgacaagc tggcgatgtt ctacctggcc gccaaccggg
      481 acgaggacgt gttcgacgac ccgcaccgct tcgatgtgcg ccgctcaccg aacaaccacg
      541 tcggcttcgg tggtcccggc ccgcacttct gcctcggtgc gcacctggcc cgccgggaga
      601 tcacggtcat gttccgggag ctgttccacc ggctgcccga catccaggca tcgggtccgc
      661 ccgagctgct gctgtcgagc ttcatccacg gcgtgaagca cctgccggcc accttcaccc
      721 ccggtggagc ctgacgcgta gcctccgtcg catggccgac atcagcgtca cccgagagat
      781 cgccgcaccg cccgagcggg tgtggggact ggtctccgac atgacccgca tgggcgagtg
      841 gtcgcccgag gccaccggcg ggacgtggct gaagggcgcc gccggaccgg ccgccggtgc
      901 gcggttcaag ggcaccaacg aagccggcgg gaagcgttgg gccaccacct gtgaggtcgt
      961 cgagtgcgag cccggcgagc gcttctcgtt ccgcgtcacc ggcgggccgt tcaaggtcgc
     1021 cacctggtcc tacgacttcg agccgaccga ggccgggacc cgggtcacgg agtcctggaa
     1081 ggacgagcgc cacccggtga tggccaagat cttcgggaag gtcaccggcg tgaccgaccg
     1141 cgcggagcac aaccggacca acatggagcg gacgctcgaa gcgctggagc gggccgccac
     1201 gtcgtgacgg ctcgcgccgg ctggcgacga gcgcgccgat cggcgcgttc gtgtcgcaca
     1261 gcgacacgaa cggaccgatc ctgcacgggc cggggcggcc gtagcctgcg cccatgggaa
     1321 cccaggacga gcgggcggtg ctgcgggccg tcttcgaccg gctcgtcccg gccgatgact
     1381 ggccgtcgct gcccgacgcc gggctgttcc cgtacctcga ggagctgcgc gccgagccgc
     1441 tgacccgccg ggacgccgag gagatcgacg ccggcgtggc agcactggag cggatggccc
     1501 aggcggagca cggccagggg ctgtccgcgc tgtcgccgac cgtggtcgac gcgctcatcg
     1561 ccgacctcga ccccgaccac cgcgaggtcc tggtgcgtgc ggctgcacgg gcctactacg
     1621 ggcacccgga gggcgccggc gcccggatga tcggtcagcc gcgcgtgccg cccccggttc
     1681 ccggtgccga acccgcgctg cgcaccaccg acctgggagc cgtcgatgcc gactacgacg
     1741 tcgtggtgct cggtgccggg gccggcggtg gggtcgcggc ctgcgtgctc gccgaagcgg
     1801 gcgctcgggt gctgctcgtc gagcggggcc acgccgaggt cacgggggat cccgacgacc
     1861 tgcgcaacca tcggacgaag gcgcacgggt tgggtacccg gccggccgag ggcgagccgc
     1921 gcgtggtgga ggacggcgac ggcacgcacc tcgtcgagcg gccgtgggac cccgagtccc
     1981 acctcggcgt ccggatcgtg ggcggcggga cccgcgtcta ccaaggactc gcgtggaggt
     2041 tcctgcccga cgacctccgc atggcgagcc gctacggcgt gcccgacggg tcctcgctcg
     2101 ccgactggcc gctcacctac gacgacctgg cctcccacta cgactgggtg gagtgggagg
     2161 tgggggtgtg cggcgatccg gcgggtcacc gggccgccgg ccaccggcaa cgggggtacc
     2221 cgatgccgcc gctgcccgag gaccccgagg ccgccctgct cgccgccggc gccgaccggt
     2281 tgggcctggc caccggacgg gtgccgatgc tgctgaactc ggtgccgcgg gacgggcgcg
     2341 ccgcgtgcgg cggagcggac tgcggtgcct gcgtcggctt cccctgcccg accggcggtc
     2401 gcaacggcac gcacgacacg gtgatcccac gcgccctcgc ctcaggcgcg gtgctcatca
     2461 ccggcacccg cgccgtcgag atcactgccg gacccgatcg ggtggacggc gtgcagctgg
     2521 tcgacgagcg caccggcacg gcgcgccaca tccgggccgg gcacgtcgtg gtcgcctgcg
     2581 gcgcgatcga gaccgctcgg ttgctgctcg ccagccgcag cgacgcgcac cccggagggc
     2641 tcggcaacca gaccgatcag gtcggccgcc acctgcaggg tcacgccttc gcctcagcgt
     2701 tcgggtcctt cgacgtcgat ctcgacgggc cggggccggg cgccaccatc gccaccctcg
     2761 acctcgccca cgggatcgcc gacgacgacg gacgcccgct ggtcggcggc ggcctcgtcg
     2821 gcaacgagct ccgcaagctg gcgatcgtcc actggcagtg ggcgctgccg cccggcgcgc
     2881 cccgctgggg tgccgccggc aaggccgcga tgcgcgacac cttccgccgc accggtcacc
     2941 tcttcgcgca ggtgcaggag gtgccggtgc ccacgaaccg ggtccgcctc gaccccgacg
     3001 tgcgcgatgc gctgggtatg ccggtggccc gcctgtccgg tgccgcccac ccggagacgg
     3061 tgcgcaccgc ccgggcggtc gtcggtcggg ccgaggagtg gctcgccgcc agcggggcca
     3121 cccgcacgtg ggtcgatgac gtccccaccg gcctgctcgc cggcacccat caagccggca
     3181 cctgccgcat gggcaccgat cccgccacct ccgtcaccga ccccgacggc cgggtccacg
     3241 ggttcgggaa cctgtgggtg gccgacgcct ccgtccacgt cacgaacgga tcggtgagcc
     3301 cggcgctcac gatcatggcc ctcgcccacc gcctcgcgac gaaactggca gcggctgcgt
     3361 cacaccgctg acgcgttcca ccgtctccgg gacggaccgg tgtgacgcct ccgctgccag
     3421 tttcaggtgg ggatgatgcg gaggtcggcg acgtgggtgc cgacgtggtt ggtgatgcgc
     3481 cggcgggagt cgaagacctc ggggcggtcg gtgccgtcga cctcggcgag cacgtggccg
     3541 agcgcgacct ccgacgccgg ccgactgccg tcctcggcct tgatggcgat gccgaggccg
     3601 aggcggggga gggcggcgaa ttgcacgcct tcggcgccgg tcttcacgag cacgtcgccg
     3661 ttcgccgcag cgagtaggtc ggtgcagagc cggccggtgc cggcgacgaa ccacggctcg
     3721 gcgaccatgg cgtcggccag ccgtcgggag gcgtcggccc gctcgggtgg ccactcggtg
     3781 ggcgtcccga agccggcggc ggcggtggcg atggcgtgca gcggcatcgg gtggacgggg
     3841 atgccgcagc cgtcggtgcc ggcctcggcc gggtcgagcg cggtccgggt ggtctcggcc
     3901 agcgccgccc ggaccagctc ctgcaccggg tgctcgggcc ggatgtagcc ggtgatgtcg
     3961 acgccgaggt ggcgggcgac ggtgaggaac ccggcgtgct tgcccgagca gttgttgcgg
     4021 gcggccgtgg ccccggggtc gtggtcgcgg tgggcctgct cgacgccgca ctcgaggtcg
     4081 tcggcggagc agccgatgcg ctcgagccag acgtcgaccg cggcgacgtg gccgggttcg
     4141 ccgttgtggg aggcgcaggc gagggcgagc tgcaccggcc cgaggtcgaa ggcgtcggcg
     4201 gcaccggtct cgatcagcgg cagggcctgc aggggcttca ccgccgagcg ggggtgggtg
     4261 agccgatccg ggtcgcccca ggaccgcacg acgttgccct cgacgtcggc gac
//
LOCUS       DATISU010000241        16194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_664539, whole genome shotgun sequence.
ACCESSION   DATISU010000241 DATISU010000000
VERSION     DATISU010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 16194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16194
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664539"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VLR27_09630"
     CDS             complement(<1..191)
                     /locus_tag="VLR27_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HSP03750.1"
                     /translation="MTDEIDYVVDDPVATITLRRPATLNALTHPMLRAFREAVDAAAA
                     DPRVVGIVVTGEGRGFCSGL"
     gene            complement(188..844)
                     /locus_tag="VLR27_09635"
     CDS             complement(188..844)
                     /locus_tag="VLR27_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP03751.1"
                     /translation="MSAGARLAIVRAAEELFASEGIEGPSLREIARRAGQGNTNAAQY
                     HFGDRDGLLLAVLDRHRDRVESRRGELLDLVEGSDPVEPRALSTALVAPLVAELSEPD
                     GGAAHLQIVAEVLARPVRFADTLEAHWRAPSIGRWSQLVEPLLPVEAVGRPLHRRFAV
                     VRFVHGELASRARERGGRGDHRLFASHLVDLVTAMLAAPVTQWTTDLIRPSTNGGRTR
                     "
     gene            complement(841..1608)
                     /locus_tag="VLR27_09640"
     CDS             complement(841..1608)
                     /locus_tag="VLR27_09640"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HSP03752.1"
                     /translation="MTDRFDLTGRVAVVTGGSRGLGRAMVQAFAEHGADVVIASRKAD
                     ACEQSAAEVRESTGRDAIGVGFHAGHWDDADRLADLVHERFGRCDVLVNNAGMSPLYG
                     SLSEVSEELFDKVVGVNFKGPFRLSALLGERMAAGEGGSLIHVSSIAAVQPTPNELVY
                     AGAKAALDAMSIGLSRAYAPKVRSNVIMPGPFLTDIAKAWDMDAFTETARRTIPLQRG
                     GEPHEVVGAALYLASDASSYTTGSVIKIDGGAAFAPG"
     gene            complement(1605..2903)
                     /locus_tag="VLR27_09645"
     CDS             complement(1605..2903)
                     /locus_tag="VLR27_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP03753.1"
                     /translation="MAWDFRTDPDYQTQLDWVDDFVREEVEPLDLVLGDPYDKSDEAA
                     MAVVRPLQQQVKERGLWAAHLGPELGGQGYGQLKLALLNEILGRSRWAPSVFGCQAPD
                     SGNAEILAHYGTEEQKAKYLQPLLDGQISSCYSMTEPHAGADPTLFTTRAVQDGDEWV
                     INGEKWFSSNARFASFYIVMAVTDPDVSAYQGMSMFVVPAETPGVEIVRNVGVGTEPE
                     GHGSHGYVRYTDVRVPADHLLGGAGQAFAIAQTRLGGGRIHHAMRTIAQVRRAFDMMC
                     ERALSRETRTGPLASLGVVQEQIADSWIEIEQFRLLVLRTAWLIDEHQDYKRVRKDIA
                     AVKVAMPKVFHDVVQRAMHLHGALGVSNEMPFVSMMVGAEIMAIADGPTEVHKITIAR
                     QVLKEHKPVDTLFPSAHLPTRRDEARAKMADVLEHVVGNQ"
     gene            2965..3603
                     /locus_tag="VLR27_09650"
     CDS             2965..3603
                     /locus_tag="VLR27_09650"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSP03754.1"
                     /translation="MRLVLVRHGDAHAGFHGVVGGRTGCAGLTPLGRRQAEALRDHLA
                     GSGRLQADALVSSVLPRAVETAQIIAPGLGIELGAQECDLCEVHTGEADGLTWTEYGT
                     RYGGFDMEAEPDRVFAPGGDSWNSFHERVGTMLGRLRRDHAGQTVVAVCHAGVIMASL
                     RVLLGIPHPGTGAQLRPTNTGLTEWEHDDAIDRWTLHSFNETAHLLELARET"
     gene            3641..4093
                     /locus_tag="VLR27_09655"
     CDS             3641..4093
                     /locus_tag="VLR27_09655"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HSP03755.1"
                     /translation="MDDVQEYSVTTARQAAERDELGDWVADFLASPGSDNAELGEQLT
                     SRPRWWAGPVPLPLDQLNRLAGPPGAPVMREVDEDDWRDDVDELAAEIADGHEPPPVI
                     VTHQDGQLVLEDGNHRVEALRRAGRTEAWGVVAFDDPDDRDAFTPPTA"
     gene            4154..5323
                     /locus_tag="VLR27_09660"
     CDS             4154..5323
                     /locus_tag="VLR27_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSP03756.1"
                     /translation="MPHRRITSALLAATLLLVACGDDDDTSTPPTSEEVATETETETE
                     AASDAEAEADAEPEPEAPAADQPGPYAVGRSTQQVTDPDRPERTLTVEVWYPAAAPAE
                     PTPYELIPGVEFAADLSTVDAPPSDEAPFPVLVFSHGSGGLRQQSATIVETVASHGFV
                     VVAADHAGNTAIDRLLGTETDFGVSARNRVLDVVLLLDEVEAGRLAGGLADPDRIAVM
                     GHSFGGFTALAVAGGYDDIAADPRVDAIVPLAPASSRLTDEALSSIGIPMLIVTGSDD
                     ATTPVEDQSTRPLELATGPATLVEIEGGSHEVVTNVCDILDAVSAPGAEVPDGTVEAA
                     QALAGETCEPDAAVDVAQAFDITERYAVSFLRQHLADDERYEAVEPADGATVRAG"
     gene            5425..5970
                     /locus_tag="VLR27_09665"
     CDS             5425..5970
                     /locus_tag="VLR27_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HSP03757.1"
                     /translation="MTTLHERNTTWDELAEAHRRARPATGPTDGVPAHRPQVAVLTCA
                     DARVSPARLFDLPAGALFTVRVAGASATTESVASLSYAVEQLGVRTVVVLGHTHCGAV
                     AAAVDGVDDPAIESLVAPIRPVVTPACEEVHCAVPAHVTATVAALRDDPGPLGAALRS
                     GDAEVHGAIVDVVDGSLTRLT"
     gene            6008..6619
                     /locus_tag="VLR27_09670"
     CDS             6008..6619
                     /locus_tag="VLR27_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSP03758.1"
                     /translation="MTTTDPQHATATIDPAELTRMRADDPAVHVLDVRTPGEYETGHI
                     PGSYNVPLDLLGEHVRDLAALDHKVVLVCQSGARATKALETLVEAGKSNLRLLQGGMN
                     AWTSSGGDVARTNDTRWAMDRQVRLVAGSVTLASVVASIWFPPARFLAGFIGAGLTFS
                     AVTNTCGMAMMLSKLPYNRGAGCDMDAVLDQLTTAETSNRTGA"
     gene            6622..8001
                     /locus_tag="VLR27_09675"
     CDS             6622..8001
                     /locus_tag="VLR27_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSP03759.1"
                     /translation="MIFNQYYLDCLSHASYLIADETTGKAVVVDPQRDISEYLTDAED
                     KGLTIELVLETHFHADFLSGHLELAQATGAQIGYSSVAETEFEIRPLADGERISLGEV
                     ELEIRHTPGHTPESMSIVIYEHADDETPYGVLTGDTLFIGDVGRPDLLASVGFTRDEL
                     ATKLYDSLHDKLLTLPDATQVFPAHGAGSACGKNLSTELTSTIGAQRQENYALLAPDR
                     TSFIDLVTEGQPPAPGYFIHDATLNKMQRPLMDEAAPPQPMSWEQVQAAMEAGALLVD
                     GRDPVEFAHGHLKGAVNVGLEGRYAEFAGSVVPVDADIVLFVEPGAELEAKNRLGRIG
                     FDRVLGFVDRPFEVLLAHQDQAQVASRLTPVDFEERRKDLGDIQLVDVRNPGEHALGA
                     LPDSMSIPVGQLPNRLDELDPTRPTVVYCAGGYRSSVAASLLRANGFDDVSDIVGGYG
                     AWTEVVQPA"
     gene            8010..9764
                     /locus_tag="VLR27_09680"
     CDS             8010..9764
                     /locus_tag="VLR27_09680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 26 protein"
                     /protein_id="HSP03760.1"
                     /translation="MTTPPTTVGQRLRGMRPRVSRDTLLSDLGAGLAVGAMLVPQGMA
                     YALLAGLPPEVGLYASTVPLVLYAIFGTSRQLAVGPVAIVSLLSATTIASVADEGTAG
                     YVTAAALLALLVGVVHIVLGVARLGFLTRLLSHPVLVGFTSAAALIIGASQVKHLLGV
                     SLENDEHFHETVWELIQKSTDAHGLTLAVGLGAVALMVGLKRWLPVLPGALAAVAVTT
                     VASVVWDLESRGVKVVGEIPQGLPPLTVPDEFGIIGTLLPAAMVITLVGYMESIAVAK
                     VYARRNRYEVDANRELIGLGAANVGAGLFGGYPVTGGFSRTAVNAEAGARTKLAALIT
                     AGVVLLVIVALTPLFEKLPSATLGAIVVVAVAKLFDLAEISHIRKLKTADFATLVVAF
                     VATLAFGVELGIGIAIVASMVVVAVRMMTPHTAELGRLPGSSLYRNVSRFPQAERVPG
                     VAIVRFDVSLSYLNVEFLKRRVQRLVDESGPELRAVVLDASGVNDIDTSAVETLAELI
                     TDLDEQGITLHLASAKGPVRDVLMRAGTYQQLGNRVHDQVHDAVAAVATDQVDPHGVT
                     PPGVPTETGPSARPESRS"
     gene            9764..10036
                     /locus_tag="VLR27_09685"
     CDS             9764..10036
                     /locus_tag="VLR27_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HSP03761.1"
                     /translation="MLQIPEETTMDLVKRLRRIEGQVRGIQAMLTEQRDCGDVVTQIA
                     AANKALEQVGFKLVASGLTYCISHPDEAADSGYDLARVEKMFTKIS"
     gene            10050..11402
                     /locus_tag="VLR27_09690"
     CDS             10050..11402
                     /locus_tag="VLR27_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSP03762.1"
                     /translation="MLDIRIVETPGLGDRSYIATDGEVAVVIDPQRDIDRILEEVGDV
                     PVTHVLETHIHNDYVTGGLVLAERCGATYVVNADDPVDFDRHGVRDGDVLTTGSLEIT
                     VIHTPGHTPTHLSYAVGVDGETADAVFTGGSILFGAMGRTDLVDPNITEDLTRAQHRS
                     ARRLAEMLPDDTDIFPTHGFGSFCSATETKGTESTIGKEKEQNLALTEDEDGFVTQLM
                     AGLTAYPRYYAQMGPLNLAGPDEPDLDMPQLVDPAALQHRIEHGEWVVDLRDRKAYAA
                     DHVQGTVSIEYADPFATHLGWAVPWDAPLTLIGDSPDDVIGARRQLARIGIDQMAAAV
                     GTTDELAADGTTSSYRRATFQDLAAERAERGDEIVVLDVRRPDEWEQGHIAGAVHIPF
                     YDLRGRQGDVPDGEVWVHCQSGYRASIGASLMDGVGREVVHIDDEWDNAETAGNPIES
                     "
     gene            11426..12025
                     /locus_tag="VLR27_09695"
     CDS             11426..12025
                     /locus_tag="VLR27_09695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP03763.1"
                     /translation="MDRDAWNERYEAADLVWTAGPNQTFADEVAGLEPGTALDLAAGE
                     GRNAIWLATQGWTATAVDFSDVALDKARKLAEASEVEIETVVADVTTHVPPAGAYDLV
                     AVIYLHLPEDERRLVHQRAAAAVAPGGTMVVLGHDTTNLTDGHGGPQDASVLYTPDDV
                     VADLEGSGLTVTKAERVDRTVTTPDGDRTAIDALVVARR"
     gene            complement(12103..12468)
                     /locus_tag="VLR27_09700"
     CDS             complement(12103..12468)
                     /locus_tag="VLR27_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HSP03764.1"
                     /translation="MQTFTDHQARSGATRLHLSGEQLARLRAEVQGTLEDHRRHLQEN
                     EELFGVLVSDPSVDAGERQAARVAAAKATEVCEEAERALAAMDDGAYGRCTGCGQDIP
                     FERLEALPLTQSCVACPQP"
     gene            12665..13498
                     /locus_tag="VLR27_09705"
     CDS             12665..13498
                     /locus_tag="VLR27_09705"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP03765.1"
                     /translation="MGIPPDLETLVDRYATITLEELISSDPERAAEAVERAASLGWDL
                     HQPRAVLLASIDPPVDPAVARRVLATVSAAARATLGDEAIVWTRATSVAALLVADDRT
                     AAQRRLLADALREELDERVRNVTLSIGVGRGVEDPLLMSSSFVDARRAVEVGRWAKGR
                     HVTELYDELGLERLFASVPQPDLHDFVLHAIGPLLDHDRRHRTELVETLSTWLEARNM
                     AEAARQMFVHYNTFKNRLERIEAILGPVLSDPSRLLECEVAIHVSRHYDGPWRTAPTD
                     G"
     gene            complement(13506..13847)
                     /locus_tag="VLR27_09710"
     CDS             complement(13506..13847)
                     /locus_tag="VLR27_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HSP03766.1"
                     /translation="METTDRGRTMTEAQLQELSGQLQQIVVTHRRRLRRQQESFRSLP
                     LHDRSSARRRQMARRAAMTSFRVCEEASRALDALEDGSYGSCDRCSCAIAFEELRARP
                     LTRRCRECDPA"
     gene            complement(13996..15294)
                     /locus_tag="VLR27_09715"
     CDS             complement(13996..15294)
                     /locus_tag="VLR27_09715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP03767.1"
                     /translation="MSDTVSPPPTAAVPAGIQLGLRENLPQFGLLVLVNALVGATVGQ
                     ERTVLPLLAEDVFGLSAYSAMLSFIAVFGVSKAITNLFAGALGDKYGRKPVLVVGWLV
                     AIPVPLLLIWAPTWGWVLFANVLLGINQGLTWSLTVIMKIDLVGPARRGLAMGLNEGA
                     GYGAVAAAAWATGWIASEYGLRPEPFYLGIGLVIIGIGLSTLFVRETSHHVAHEAANH
                     VQRDGHPDAELTPRQVFALSTWRNPSLSAASQAGLVNNLNEGMAWGLFPLFFAAGGVS
                     VGKIGGLIAIAPAVWGFGQLFTGALSDKVGRKWLIAGGQFTEAAGLTIIALGDGFGVW
                     ALGSAVFGAGTAMAYPTLIAAIGDVAHPTWRASAVGVYRLWRDLGFAVGAVLAGVLAD
                     VVNIEFAIFIVAGITAASGLDVSLRMRETLHRPPLPVVPD"
     gene            complement(15291..15968)
                     /locus_tag="VLR27_09720"
     CDS             complement(15291..15968)
                     /locus_tag="VLR27_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSP03768.1"
                     /translation="MAPEAKAALFDQFAMVGKAFANGRRAELVDVLAQGERAVEALAD
                     QIGQSVANTSQHLQVLARAGLVTSRRDGNRVLYRLADEQVEALWQALRVVAVANVSGV
                     RQAAEDYLGDRDGLEQVTAHELARRLGEGVLVWDVRPTSEYEAGHVPGAVSVPPDEVQ
                     RRLDDVPDDVEVVAYCRGRFCVYADDAVRALVAAGRRAVRLDEGFPEWRRAGLPVAVG
                     TDRRSDP"
BASE COUNT         2371 a         6147 c         5524 g         2152 t
ORIGIN      
        1 aggccggagc agaacccccg gccctcgccg gtgaccacga tgcccacgac tcgggggtcg
       61 gctgcggcgg cgtcgaccgc ctcgcggaag gcgcgcagca tggggtgcgt cagggcgttg
      121 agggtcgccg gacggcgcag cgtgatggtg gcgacggggt cgtccaccac gtagtcgatc
      181 tcgtcggtca tcgcgttcgt cctccgttgg tgctcgggcg gatcaggtcg gtggtccact
      241 gcgtcaccgg tgcggccagc atggccgtca ccaggtcgac gaggtggctg gcgaacaggc
      301 ggtggtcacc gcggccgccc cgctcgcggg cccggctggc cagctcgccg tgcacgaaac
      361 ggaccacggc gaagcggcgg tggaggggac ggccgaccgc ctcgacgggc agcaggggct
      421 ccaccagctg gctccaccgg ccgatgctcg gcgcccgcca gtgggcctcg agggtgtcgg
      481 cgaagcgcac ggggcgggcg agcacctcgg ccacgatctg gaggtgggcg gccccgccgt
      541 cgggctcgga cagctcggcg acgagcgggg cgaccagcgc ggtcgacagg gcccggggct
      601 cgaccggatc cgacccctcg accaggtcca gcagctcgcc gcgccgggac tcgacccggt
      661 cgcggtgcct gtcgaggacg gccagcagca acccgtcccg gtccccgaag tggtactggg
      721 cggcgttggt gttgccctgg ccggcccgcc gggcgatctc ccgcagcgac gggccctcga
      781 tgccctcgga cgcgaacagc tcctcggccg cccggacgat ggcgaggcgg gccccggcgc
      841 tcatcccggt gcgaacgccg cgccgccgtc gatcttgatg accgacccgg tcgtgtagct
      901 cgaggcgtcg ctggccaggt agagggcggc acccacgacc tcgtggggtt caccgccacg
      961 ctggagcggg atggtgcgcc gggccgtctc ggtgaaggcg tccatgtccc aggccttggc
     1021 gatgtcggtg aggaacgggc ccggcatgat cacgttgctg cggaccttcg gcgcgtaggc
     1081 ccggctgagc ccgatcgaca tcgcgtcgag ggcggccttg gcgccggcgt acaccagctc
     1141 gttcggcgtc ggctgcacgg cggcgatgga gctgacgtgg atcagcgagc caccctcgcc
     1201 ggccgccatc cgctccccca gcagcgccga caggcggaac ggccccttga agttgacgcc
     1261 caccaccttg tcgaacagct cctcggagac ctccgacagc gacccgtaca gcggcgacat
     1321 gccggcgttg ttgacgagca cgtcgcaccg gccgaagcgc tcgtgcacga ggtcggccag
     1381 ccggtcggcg tcgtcccagt gccccgcgtg gaagccgacc ccgatcgcgt cgcgaccagt
     1441 cgactcccga acctcggcgg cggactgctc gcacgcgtcg gccttgcggc tggcgatgac
     1501 gacgtcggcg ccgtgctcgg cgaaggcctg gaccatcgcc cgacccagcc cccggctgcc
     1561 cccggtgacg accgcgaccc gaccggtgag gtcgaaccga tccgtcactg gttgccgacc
     1621 acgtgctcga gcacgtcggc catcttggcg cgggcctcgt cgcggcgggt cgggaggtgc
     1681 gcggagggga acagcgtgtc gaccggcttg tgctccttca gcacctggcg ggcgatcgtg
     1741 atcttgtgga cctcggtggg gccgtcggcg atcgccatga tctcggcgcc gaccatcatc
     1801 gacacgaacg gcatctcgtt ggagacgccc aatgcgccgt gcaggtgcat cgcccgctgc
     1861 accacgtcgt ggaacacctt gggcatcgcc accttcacgg ctgcgatgtc cttgcggacc
     1921 cgcttgtagt cctggtgctc gtcgatgagc cacgccgtgc gcaggacgag gaggcggaac
     1981 tgctcgatct cgatccagga gtcggcgatc tgctcctgca cgacgccgag cgacgccagc
     2041 ggaccggtgc gggtctcgcg gctgagggcc cgctcgcaca tcatgtcgaa ggcccgacgg
     2101 acctgggcga tggtccgcat ggcgtggtgg atgcgaccgc cgccgaggcg ggtctgggcg
     2161 atggcgaagg cctgcccggc gccgccgagc aggtggtcgg ccggcactcg cacgtcggtg
     2221 tagcggacgt agccgtggga gccgtgaccc tcgggctcgg tgccgacgcc gacgttgcgg
     2281 acgatctcca cgccgggcgt ctcggccggc accacgaaca tgctcatgcc ctggtaggcg
     2341 ctcacgtcgg ggtcggtgac cgccatgacg atgtagaagg acgcgaagcg ggcgttggag
     2401 gagaaccact tctccccgtt gatcacccac tcgtcgccgt cctgcacggc acgggtggtg
     2461 aacagggtcg gatccgcacc ggcgtggggc tcggtcatcg agtagcacga cgagatctgg
     2521 ccgtcgagca gcggctgcag gtacttcgcc ttctgctcct cggtgccgta gtgggcgagg
     2581 atctcggcgt tgcccgagtc cggggcctgg cagccgaaca ccgacggcgc ccagcgggag
     2641 cggccgagga tctcgttgag cagcgccagc ttcagctggc cgtagccctg cccgccgagc
     2701 tcgggaccca ggtgggcggc ccagagcccg cgctccttga cctgctgctg cagcggacgg
     2761 acgacggcca tcgcggcctc gtcggacttg tcgtaggggt cgccgaggac gaggtccagc
     2821 ggctcgacct cctcacggac gaagtcgtcc acccagtcga gctgggtctg gtagtcgggg
     2881 tcggtgcgga agtcccatgc catgcgccga cgttaacgca cgtgcgttaa cagcgcggcg
     2941 gcccgcctcg tagcgtgggc cagcatgcga ctcgtcctcg tccgccacgg tgacgcccac
     3001 gccgggttcc acggcgtggt cgggggccgc accggctgcg ccggcctgac cccgctcgga
     3061 cgacgccagg ccgaagccct gcgcgaccac ctcgccggct ccggccgcct ccaggccgac
     3121 gccctcgtct ccagcgtgct ccctcgcgcc gtggagacgg cgcagatcat cgcaccgggc
     3181 ctgggcatcg agctcggtgc ccaggagtgc gacctgtgcg aggtccacac cggcgaggcc
     3241 gacggcctga cctggaccga gtacggcacg cggtacgggg gcttcgacat ggaggccgag
     3301 ccggaccggg tgttcgcacc gggtggcgac agctggaaca gcttccacga gcgggtgggc
     3361 accatgctcg gccgcctccg ccgcgaccac gccgggcaga ccgtcgtcgc cgtgtgccac
     3421 gccggcgtga tcatggcgtc gctgcgggtg ctgctcggca tcccccaccc gggcaccggc
     3481 gcccagctgc gtccgacgaa caccgggctc accgagtggg agcacgacga cgccatcgac
     3541 cgctggacgc tgcactcatt caacgagacc gcccacctgc tcgagctcgc ccgggagacc
     3601 tgagcggtcg agatcccccg cgccggggaa cagagccacg atggacgacg tgcaggagta
     3661 ctcggtgacg accgcccggc aggccgccga gcgtgatgag ctcggcgact gggtcgccga
     3721 cttcctggcc agcccgggca gcgacaacgc cgagctgggc gagcagctca cgtcccggcc
     3781 ccgctggtgg gccgggcccg tgccgctccc cctggaccag ctgaaccggc tggcgggccc
     3841 gcccggagcg ccggtgatgc gagaggtcga cgaggacgac tggcgcgacg acgtggacga
     3901 gctcgccgcc gagatcgcgg acggccacga accgccaccg gtgatcgtca cccaccagga
     3961 cggccagctc gtcctcgagg acggcaacca ccgggtcgaa gcgctgcggc gggcggggcg
     4021 gaccgaggcg tggggtgtgg tcgccttcga cgaccccgac gaccgggacg cgttcacccc
     4081 gcccaccgcc tgacctcgtg accggctgaa cggccgaccg agcgggcgcg acggcggtcg
     4141 gtaccgtgcc gcggtgcccc atcgccgcat cacctccgcc ctgctcgccg ccacgctcct
     4201 gctcgtcgcg tgcggtgacg acgacgacac gtcgacgcca ccgaccagcg aggaggtcgc
     4261 caccgagacc gagaccgaga ccgaggccgc gtcggacgcc gaggccgagg ccgacgccga
     4321 gcccgagccc gaggctcccg cggcggacca gcccggcccg tacgccgtcg gtcggagcac
     4381 ccagcaggtc accgaccccg accgtcccga gcggaccctc accgtcgagg tctggtaccc
     4441 ggccgcggcg ccggccgagc ccacgccgta cgagctgatc cccggcgtcg agttcgccgc
     4501 cgacctgtcg acggtggatg ccccaccgtc ggacgaggcc ccgttcccgg tgctggtgtt
     4561 ctcccacggc tccggcggcc tgcgccagca gtcggccacg atcgtggaga cggtggcatc
     4621 gcacgggttc gtggtcgtcg ccgccgacca cgcgggcaac accgccatcg accgcttgct
     4681 cggcaccgag accgacttcg gcgtgtcggc acgcaaccgc gtcctcgacg tggtgctcct
     4741 gctcgacgag gtggaagccg gacgcctcgc cggcggcctg gccgatccgg accggatcgc
     4801 ggtgatgggt cactccttcg ggggcttcac cgccctcgcc gtggccggtg ggtacgacga
     4861 catcgccgcc gacccgcgcg tggacgcgat cgtccccctc gccccggcct cgtcccggct
     4921 gaccgacgag gcgctgtcgt cgatcggcat cccgatgctg atcgtgaccg gcagcgacga
     4981 cgccaccacc ccggtggagg accagtcgac ccgcccgctc gagctcgcga ccgggccggc
     5041 gaccctcgtc gagatcgagg gcggctccca cgaggtggtc accaacgtct gcgacatcct
     5101 cgacgccgtc agcgcgccgg gggccgaggt ccccgacggg accgtcgagg ccgcgcaggc
     5161 gctcgccggc gagacctgcg agcccgacgc cgccgtcgac gtggcgcagg ccttcgacat
     5221 caccgagcgc tacgccgtgt cgttcctccg ccagcacctc gccgacgacg agcgctacga
     5281 ggcagtcgag cccgccgacg gagccacggt gcgcgccggc tgaccccgcc ccgccccggc
     5341 gatcgcggcc gggggtagcc aaacattgtc cggacatgta ggttggtgca taccccaccg
     5401 ggtatcaccg acggagcttg gacgatgacc accctgcacg agcgcaacac cacctgggac
     5461 gagctggcgg aggcacaccg ccgtgcccgc cctgcgaccg gtcccaccga tggcgtcccg
     5521 gcccaccgcc cgcaggtcgc ggtgctcacg tgcgccgatg cccgggtctc accggcccgg
     5581 ctgttcgacc tcccggccgg cgccctgttc accgtccgcg tcgccggcgc ctcggccacg
     5641 accgagtcgg tcgccagcct gagctacgcc gtcgagcagc tcggggtgcg caccgtggtc
     5701 gtcctcggcc acacccactg cggcgcggtg gccgcggcgg tggacggcgt ggacgacccc
     5761 gccatcgagt ccctcgtggc gccgatccgc cccgtggtga cccccgcctg cgaggaggtg
     5821 cactgcgccg tcccggcgca cgtcaccgcc accgtcgccg cgctccgcga cgaccccgga
     5881 cccctcggcg cggcgctgcg cagcggcgac gccgaggtcc acggcgccat cgtcgacgtc
     5941 gtcgacggct ccctgacccg cctcacctga ccccccgaaa acccttcccc cacaggagac
     6001 acaccccatg acgaccaccg atccccagca cgccaccgcc accatcgatc cggccgagct
     6061 cacccgcatg cgggccgacg acccggcggt gcacgtcctc gacgtgcgca cgcccggcga
     6121 gtacgagacc ggccacatcc ccggctccta caacgtcccc ctcgacctgc tcggcgagca
     6181 cgtgcgcgac ctggccgccc tcgaccacaa ggtcgtcctc gtctgccagt cgggcgcccg
     6241 ggccaccaag gccctcgaga ccctcgtcga ggcgggcaag tccaacctgc gcctcctcca
     6301 gggcggcatg aacgcctgga cctcgagcgg cggcgacgtc gcccggacga acgacacccg
     6361 ctgggcgatg gaccgccagg tgcgcctggt cgccggcagc gtcacgctgg ccagcgtggt
     6421 cgccagcatc tggttcccgc ccgcccgctt cctcgccggg ttcatcggcg ccggcctcac
     6481 cttctccgcc gtcaccaaca cctgcggcat ggccatgatg ctctcgaagc tcccctacaa
     6541 ccggggcgcc ggatgcgaca tggacgccgt gctcgaccag ctcaccaccg ccgagacctc
     6601 caaccgcaca ggagcatgac catgatcttc aaccagtact acctcgactg cctctcccac
     6661 gcgtcgtacc tcatcgccga cgagaccacc ggcaaggccg tggtcgtcga cccccagcga
     6721 gacatctccg agtacctcac cgatgccgag gacaagggcc tcaccatcga gctcgtgctc
     6781 gagacccact tccacgccga cttcctgtcc ggccacctcg agctcgccca ggcgaccggc
     6841 gcccagatcg ggtactcctc ggtcgccgag accgagttcg agatccgccc gctggccgac
     6901 ggggagcgca tctccctcgg cgaggtcgag ctcgagatcc gccacacccc gggccacacg
     6961 cccgagtcga tgagcatcgt catctacgag cacgccgacg acgagacccc ctacggcgtc
     7021 ctcaccggcg acaccctgtt catcggcgac gtcggccgcc ccgacctgct cgcctcggtg
     7081 ggcttcaccc gggacgagct ggccaccaag ctgtacgact cgctccacga caagctgctc
     7141 acgctgcccg acgccaccca ggtgttcccc gcccacggcg ccggctccgc ctgcggcaag
     7201 aacctgtcga ccgagctgac ctccaccatc ggggcccagc ggcaggagaa ctacgccctg
     7261 ctcgcgccgg accgcaccag cttcatcgac ctcgtcaccg agggtcagcc gccggcgccc
     7321 ggctacttca tccacgacgc cacgctgaac aagatgcagc gcccgctgat ggacgaggcc
     7381 gccccgcccc agccgatgtc gtgggagcag gtgcaggccg ccatggaggc gggtgccctc
     7441 ctcgtcgacg ggcgcgaccc cgtcgagttc gcccacggcc acctcaaggg cgcggtcaac
     7501 gtcggcctcg agggccgcta cgccgagttc gccggatccg tggtgccggt ggacgccgac
     7561 atcgtcctgt tcgtcgagcc cggtgccgag ctcgaggcca agaaccgcct gggccgcatc
     7621 ggcttcgacc gggtgctcgg cttcgtcgac cgccccttcg aggtgctgct cgcccaccag
     7681 gaccaggccc aggtggcctc gcggctcacg ccggtcgact tcgaggagcg ccgcaaggac
     7741 ctcggcgaca tccagctcgt cgacgtccgc aacccgggcg agcacgctct cggggccctt
     7801 ccggactcca tgagcatccc cgtcggccag ctcccgaacc gcctggacga gctcgacccc
     7861 acccgaccga ccgtcgtgta ctgcgccggc ggctaccgct cgtccgtcgc ggcctcgctg
     7921 ctccgggcca acggcttcga cgacgtcagc gacatcgtcg ggggctacgg cgcctggacc
     7981 gaagtcgtcc agccggcctg agtcgtgtca tgaccactcc acccaccacc gtcgggcaac
     8041 ggctccgggg catgcgaccc cgggtctccc gcgacaccct gctctccgac ctcggagccg
     8101 gcctggcggt cggggccatg ctcgtgcccc agggcatggc ctacgccctg ctcgccgggc
     8161 tcccacccga ggtcggcctc tacgcctcga cggtgccgct ggtcctctac gcgatcttcg
     8221 gcacctcgag gcagctggcg gtcggccccg tcgccatcgt ctccctgctc agcgcgacga
     8281 ccatcgccag cgtcgccgac gaggggaccg ccggctacgt caccgcagcc gcgctgctcg
     8341 cgctgctcgt cggcgtggtg cacatcgtgc tgggcgtggc ccgcctcggc ttcctcacca
     8401 ggctgctgtc gcacccagtc ctcgtcggct tcacctctgc agccgcgctg atcatcggtg
     8461 ccagccaggt caagcacctc ctcggcgtgt ccctggagaa cgacgagcac ttccacgaga
     8521 ccgtgtggga gctgatccag aagtccaccg atgcccacgg cctcacgctg gccgtcggtc
     8581 tcggggccgt cgcgctgatg gtcgggctca agcgctggct cccggtgctc cccggcgccc
     8641 tcgcggccgt ggccgtgacg accgtcgcct ccgtcgtgtg ggacctcgag agccgagggg
     8701 tcaaggtcgt gggagagatc ccccagggcc tgccgccgct gaccgtgccg gacgagttcg
     8761 gcatcatcgg caccctgctc ccggcagcga tggtcatcac cctcgtcggc tacatggaat
     8821 cgatcgcggt cgccaaggtc tacgcccgac gcaaccgcta cgaggtcgat gccaaccgcg
     8881 agctcatcgg gctcggtgcg gccaacgtcg gcgcaggcct cttcggcggc tacccggtca
     8941 ccggcggctt cagccgcacc gcggtgaacg ccgaggccgg cgcccggacc aagctcgcag
     9001 cgctgatcac cgcgggcgtc gtcctcctgg tcatcgtcgc cctcaccccg ctgttcgaga
     9061 agctcccctc ggccaccctc ggcgcgatcg tcgtcgtggc cgtcgccaag ctgttcgacc
     9121 tcgccgagat ctcccacatc cggaagctga agacggccga cttcgccacc ctggtcgtgg
     9181 cgttcgtcgc caccctggcc ttcggcgtcg agctcggcat cggcatcgcc atcgtcgcct
     9241 cgatggtggt cgtggccgtg cggatgatga ccccccacac cgccgagctc ggtcgcctcc
     9301 cgggcagctc gctctaccgc aacgtgagcc gcttcccgca ggccgagcgg gtgcccggcg
     9361 tggccatcgt ccgcttcgac gtgtcgctgt cgtacctgaa cgtggagttc ctgaagcgtc
     9421 gggtgcagcg cctggtggac gagagcggac cggagctccg ggccgtggtg ctcgacgcca
     9481 gcggcgtgaa cgacatcgac acctccgccg tcgagaccct cgccgagctg atcaccgacc
     9541 tcgacgagca gggcatcacg ctgcacctgg cgagcgccaa gggcccggtc cgcgacgtgc
     9601 tcatgcgagc gggcacctac cagcagctcg ggaaccgggt ccacgatcag gtccacgacg
     9661 cggtcgcggc agtcgcgacc gaccaggtag acccgcatgg agtcacaccc cctggggtac
     9721 cgaccgaaac cgggccgtcg gcccgaccgg agagcaggag ctgatgttgc agatccccga
     9781 agagaccacc atggacctgg tcaagcggct ccgccgcatc gagggccagg tccgcggcat
     9841 ccaggcgatg ctgaccgagc agcgcgactg cggcgacgtc gtcacccaga tcgctgcagc
     9901 caacaaggcg ctggagcagg tgggcttcaa gctcgtcgcc tccggcctca cctactgcat
     9961 ctcgcatccg gacgaggcgg ccgacagcgg gtacgacctg gcccgtgtcg agaagatgtt
    10021 caccaagatc agctgagagg aacccagacg tgctcgacat ccgcatcgtg gagacccccg
    10081 gactcggcga ccgcagctac atcgccaccg acggcgaggt cgcggtcgtc atcgaccccc
    10141 agcgcgacat cgaccggatc ctggaggagg tcggggacgt cccggtcacc cacgtcctcg
    10201 agacccacat ccacaacgac tacgtcacgg gcggcctggt gctcgccgag cgctgcgggg
    10261 ccacctacgt cgtcaacgcc gacgatccgg tcgacttcga ccgccacggc gtccgcgacg
    10321 gcgacgtgct caccaccggc tcgctcgaga tcaccgtcat ccacacgccg gggcacaccc
    10381 ccacgcacct gtcctacgcc gtgggcgtcg acggcgagac ggccgacgcg gtcttcaccg
    10441 gaggctcgat cctcttcggc gccatgggcc gcaccgacct ggtcgacccc aacatcaccg
    10501 aggacctcac ccgggcccag caccgctcgg cgcgccggct ggccgagatg ctgcccgacg
    10561 acaccgacat cttccccacc cacgggttcg gcagcttctg ctcggccacc gagacgaagg
    10621 ggaccgagtc caccatcggc aaggagaagg agcagaacct cgccctcacc gaggacgagg
    10681 acggcttcgt cacccagctc atggccggcc tcaccgccta cccccgctac tacgcccaga
    10741 tgggacccct gaacctggcc ggccccgacg aacccgacct cgacatgccc cagctcgtcg
    10801 acccggccgc cctccagcac cgcatcgagc acggcgagtg ggtggtcgac ctccgagacc
    10861 gcaaggccta cgccgccgac cacgtacaag gcacggtgtc gatcgagtac gccgacccct
    10921 tcgccacgca cctcggctgg gccgtcccct gggacgcccc gctgaccctc atcggcgact
    10981 ccccggacga cgtcatcggc gcccgccgcc agctggcccg catcggcatc gaccagatgg
    11041 ccgccgcggt cgggacgacc gacgagctcg ctgccgacgg caccaccagc tcgtaccgcc
    11101 gggccacctt ccaggacctc gccgccgagc gggccgagcg cggcgacgag atcgtcgtcc
    11161 tcgacgtccg ccggcccgac gagtgggagc agggccacat cgccggtgcg gtccacatcc
    11221 ccttctacga cctccgcggc cgccagggcg acgtgcccga cggtgaggtc tgggtgcact
    11281 gccagtccgg ctaccgggcc tccatcggcg cctccctcat ggacggcgtc ggccgcgagg
    11341 tggtccacat cgacgacgag tgggacaacg ccgagaccgc cggcaacccc atcgagtcct
    11401 gagaccccga tcccgaggag cccccatgga ccgtgacgcc tggaacgagc gctacgaagc
    11461 cgccgacctc gtctggacgg cgggcccgaa ccagaccttc gccgacgagg tcgccgggct
    11521 cgaacccggc accgcgctcg acctcgccgc cggggagggc cgcaacgcca tctggctggc
    11581 gacgcagggt tggacggcca cggccgtgga cttctccgac gtcgccctcg acaaggcccg
    11641 gaagctggcc gaggccagcg aggtcgagat cgagaccgtc gtcgccgatg tgaccaccca
    11701 cgtgcccccg gccggcgcgt acgacctggt ggccgtcatc tacctccacc tgcccgagga
    11761 cgagcgccgg ctcgtgcacc agcgcgccgc ggctgccgtc gcccccggcg ggacgatggt
    11821 cgtcctcggc cacgacacca cgaacctcac cgacggccac ggcggacccc aggacgcctc
    11881 cgtcctctac accccggacg acgtcgtggc cgacctcgag gggtcggggc tcaccgtcac
    11941 caaggccgag cgggtcgacc gcacggtcac caccccggac ggcgaccgga ccgcgatcga
    12001 cgcgctcgtg gtcgcccgcc ggtaaccggc cggcgtggtg atgggtgccc ggcgccgttg
    12061 ccgagcaccc aagaccacgt caccgctggt ccggcgacga cctcacggct gcgggcaggc
    12121 cacgcagctc tgcgtcaggg gcagggcctc gagacgctcg aacgggatgt cctgcccgca
    12181 gccggtgcag cggccgtagg cgccgtcgtc catggccgcc agggcgcgct cggcctcctc
    12241 gcagacctcg gtggccttcg ctgcggccac gcgggccgct tggcgctcgc ccgcgtcgac
    12301 cgacgggtcg ctgacgagga cgccgaacag ctcctcgttc tcctgcaggt ggcgccggtg
    12361 gtcctcgagc gtcccttgca cctcggcgcg caggcgggcc agctgctcgc cggacaggtg
    12421 gagccgggtc gcgccgcttc gcgcctggtg atcggtgaac gtctgcacga ctcgggctcc
    12481 ctcctggtgt gacgaccctc ggacacccat gaccgtaggg agggcccgat cctccggcgt
    12541 tgggggaact cgacgatctg tcgagcgtcc ggttgtcgga tctccacacc cctcgggagc
    12601 cgcatcgccg gctggcaccg tccggccttc gccccggccc cgccggactg gtcagacttc
    12661 ctcgatgggg atcccgccgg acctggagac gctcgtggac cgctacgcga ccatcacgct
    12721 cgaggagctg atctcgtcgg atccggagcg ggccgccgaa gccgtcgagc gggcggcgtc
    12781 cctcggctgg gacctgcacc agccccgggc cgtgctcctc gcgtcgatcg acccgccggt
    12841 ggatcccgcc gtggcccggc gggtcctggc caccgtctcg gcagcggctc gggcgaccct
    12901 cggcgacgag gcgatcgtgt ggacgagggc gacgtcggtc gctgccctcc tcgtcgccga
    12961 cgaccggacc gcggcgcagc gccggctgct ggccgacgcg ctccgggagg agctggacga
    13021 gcgcgtccgg aacgtcaccc tgagcatcgg ggtcggccgt ggggtggagg accccctgct
    13081 catgtcgtcg agcttcgtcg acgcccgccg cgccgtggag gtgggccgct gggccaaggg
    13141 ccgccacgtc accgagctct acgacgagct cggcctcgag cgcctcttcg cgtccgtgcc
    13201 ccagccggac ctgcacgact tcgtcctgca cgcgatcggt ccgctcctcg accacgacag
    13261 acggcaccgc accgagctgg tggagacgct gtcgacctgg ctcgaggccc gcaacatggc
    13321 cgaggccgcc cggcagatgt tcgtccacta caacacgttc aagaaccgcc tggagcgcat
    13381 cgaggccatc ctcggaccgg tcctcagcga cccgagccgg ctgctggagt gcgaggtcgc
    13441 catccacgtg tcccgccact acgacgggcc gtggcggacg gcgccgaccg acgggtgacg
    13501 ccgggtcagg ccgggtcgca ctcccggcag cggcgggtca gcggccgggc ccggagctcc
    13561 tcgaacgcga tcgcgcagct gcagcgatcg caggatccgt agctgccgtc ctccagcgca
    13621 tcgagggcgc gactggcctc ctcgcagacg cggaacgagg tcatcgccgc tcgccgggcc
    13681 atctggcgtc gccgtgcgct cgagcggtcg tgcagcggga gcgagcggaa ggactcctgc
    13741 tggcgacgca ggcgccgacg gtgggtgacg acgatctgct gcagctggcc cgagagctcc
    13801 tgcagctgcg cctccgtcat cgtccgtccg cgatcggtcg tctccatgtc ctcgtcctcc
    13861 tcctggccgc gcccgcgtgg gtccgatgac cgtacggtcg cccggacgac gccgagcagg
    13921 ccgatctcga cgatctcggc accatggaga tgtccgaacc ggacaccgct cggcgcggga
    13981 tccgagctcg cctgctcagt cgggcacgac cggcagcggc ggacggtgca gcgtctcgcg
    14041 catgcggagc gagacgtcga gtccgctggc ggcggtgatg ccggcgacga tgaagatggc
    14101 gaactcgatg ttgaccacgt cggcgaggac gccggcgagc accgccccca ccgcgaagcc
    14161 gaggtcacgc cagaggcggt agacgcccac ggcgctggct cgccaggtgg ggtgggcgac
    14221 gtcgccgatg gccgcgatga gcgtcgggta cgccatggcg gtgccggcgc cgaacacggc
    14281 gctgccgagc gcccacaccc cgaagccgtc gccgagggcg atgatcgtca agccggcggc
    14341 ctcggtgaac tgcccaccgg cgatgagcca cttgcgcccg accttgtccg agagcgcgcc
    14401 ggtgaacagc tgcccgaagc cccacacggc gggggcgatg gcgatgagcc cgccgatctt
    14461 gccgaccgag acgccaccag cagcgaagaa gagcgggaac agcccccagg ccatgccctc
    14521 gttcaggttg ttgacgaggc ccgcctggct cgccgcggag agcgacgggt tccgccacgt
    14581 cgacagcgcg aagacctgcc ggggtgtcag ctcggcgtcg ggatgtccgt cccgctggac
    14641 gtggttggcg gcctcgtggg ccacgtggtg ggaggtctcc cggacgaaca gcgtcgacag
    14701 gccgatgccg atgatgacca ggccgatgcc gaggtagaag ggctcgggtc gcagcccgta
    14761 ctcggaggcg atccagccgg tggcccacgc cgcggcggcg acggccccgt acccggcgcc
    14821 ctcgttcaac cccatggcca ggcctcgtcg ggccggcccg acgaggtcga tcttcatgat
    14881 gacggtgagc gaccacgtga ggccctggtt gatgccgagc agcacgttgg cgaacagcac
    14941 ccatccccac gtcggcgccc agatcagcag cagggggacc gggatggcga cgagccagcc
    15001 gacgacgagc acgggcttgc ggccgtactt gtccccgagc gcgccggcga agaggttcgt
    15061 gatcgccttg gacacgccga agacggcgat gaagctgagc atcgccgagt aggcggacag
    15121 gccgaagacg tcctcagcca gcagcgggag cacggtgcgc tcttggccga cggtcgcgcc
    15181 gaccagcgcg ttcaccagga cgagcagccc gaactgcggc aggttctcgc gcaggccgag
    15241 ctggatgccg gcggggacgg cggcggtcgg cggcggggag acggtgtcgc tcatggatcg
    15301 ctccttcggt cggttccgac ggcgaccggc agcccggccc ggcgccactc ggggaagccc
    15361 tcgtccaggc ggacggcacg ccggccggcg gcgacgaggg cgcgcacggc gtcgtcggcg
    15421 tagacgcaga agcggccgcg gcagtaggcg acgacctcca cgtcgtcggg gacgtcgtcg
    15481 agtcgccgct gcacctcgtc gggtggcacg gacaccgccc ccgggacgtg cccggcctcg
    15541 tactcggacg tagggcggac gtcccagacc aggacgcctt cgcccagccg ccgggcgagc
    15601 tcatgggcgg tgacctgctc caacccgtcc cggtcaccga ggtagtcctc ggcggcttgg
    15661 cgcacgccgg agacgttggc gacggccacg acgcgcagcg cctgccacag cgcctccacc
    15721 tgctcgtcgg ccaaccggta cagcacgcgg ttgccgtccc ggcgcgacgt caccagcccg
    15781 gcgcgggcca ggacctgcag gtgctgggag gtgttcgcca ccgactggcc gatctggtcg
    15841 gccagggcct cgaccgcccg ttcaccctgg gcgagcacgt ccacgagctc ggcccggcgg
    15901 ccgttggcga acgccttgcc caccatcgcg aactggtcga acagcgctgc cttcgcctct
    15961 ggtgccacgg agacaccata caccgatcac tcaagtattc aagtgagttc ttgatccgat
    16021 gccagcgcgc tcagatctca cacgcgggcg ccccggggaa gtcgacgtgg aagcccttgg
    16081 tcacccagcg gcgggtggag acgcgcagcg tcggggccag cgggacgagg tcccgggcga
    16141 cgagcacctc gaggtcgtcg cgccggagcc ggaggtaggg cgccaggttg cgcc
//
LOCUS       DATISU010000242         3934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_572026, whole genome shotgun sequence.
ACCESSION   DATISU010000242 DATISU010000000
VERSION     DATISU010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572026"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VLR27_09725"
     CDS             complement(<1..927)
                     /locus_tag="VLR27_09725"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HSP03769.1"
                     /translation="MVNHRWIGYWPNMHHFVRRHRDHLQVLAGIALGVTVAAAALLAD
                     RVVDLGVEIPTTNALSLLAATFSALVTAGAFAFWMLPLAAQLAASAVPPTIVAGRLHG
                     RFQRLVITVTLGALAYTAVAAAAIPSSEDTSAPTITTVLGAALGVAAIAALLVAIEHA
                     VRSTRPSVLVSEVAHGVIDSIRAAGRGAGGHEDDAAPAPGPSTATVVAPATGWLHAVD
                     EDALLGAMPEEATVSLQVGLGAFLVDGWTTVAIVRAEDIPEDLGAELQSHFVVDVERS
                     SEQALVGQLTQFTDIAVHAGSGSSGAPSVVYET"
     gene            complement(960..1802)
                     /locus_tag="VLR27_09730"
     CDS             complement(960..1802)
                     /locus_tag="VLR27_09730"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HSP03770.1"
                     /translation="MSFAADLPELTGLRVAQRGSRVDVTIDHGDINLLDVPLLVSLLR
                     LTEALEVADDVTVVVVSSANPDFFIAHFDVAAILELPRDGGAEDRDELGPFHVMVERW
                     RTMPCATIASIAGIVRGGGCEVVSSFDMRFAALDRAVFGQPEVALGILPGGSGTQRLP
                     HLLGRGRALEMVLGSGDIDARTAEAWGLVNRALEADALDPFVDDLADRIAASPPVAVR
                     EAKAAVLAAMPDPTAGLLEEARRFDRTLADPESQRRMERFLELGGQTPEGERDLPGLV
                     QHLT"
     gene            1911..3233
                     /locus_tag="VLR27_09735"
     CDS             1911..3233
                     /locus_tag="VLR27_09735"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSP03771.1"
                     /translation="MESSEIGMVVDSLAEGITDLHAADLSRLTARELLQLAGALEVVS
                     RRLDHARDTLAGHIDATGAHGVDGHKSAKAALVHINRVPGAEAHGSIQTARALRQLPE
                     VDAAYAAGKIPTASVRAIARVASNPRVECFLDDLVDRIFAEQASEATHDDLVSFVRDW
                     ERFADADGADQAREHAHRRRNASLVRNDLAGSWHLRGDFGDLHGASMAEVLAAYEHAE
                     WLADREVAVAEHGPGATPDQFPRTPAQRRADAMLAIFRRAAATPADAQSPEPLVNIVI
                     DQETLETELARAAGAPVDHDASRHSGRICRTTSGHPIHPADAVAAILVGHVRRVVVDA
                     ASNVIDLGRRRRCFTGSSRDAALLQTAIRDRGGTTCFWADCNSPPQWMQVDHDQTWRA
                     LGPSDIANSQTACGYHNRLKETGYRPVRGPDGTYTYRRPDGTEITPPV"
     gene            3351..3527
                     /locus_tag="VLR27_09740"
     CDS             3351..3527
                     /locus_tag="VLR27_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03772.1"
                     /translation="MADDEQERGPAREQDGPPKRYQTWNGEPCPRYVRIDGTVVGSGD
                     CLGCGTCLLGSGLV"
     gene            complement(3567..>3934)
                     /locus_tag="VLR27_09745"
     CDS             complement(3567..>3934)
                     /locus_tag="VLR27_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03773.1"
                     /translation="TFVEGLGPDLDDPDNVLFRTALRRYEDRAGFLSMCWLVDAPSGN
                     GLSIVTWDTKAACTASEPASRRTRQQVEQAFGCRIDGVEEVETIAVAAPVHDVLDLRE
                     VAGAESPFLPTKGIVQQPT"
BASE COUNT          604 a         1451 c         1391 g          488 t
ORIGIN      
        1 cgtctcgtag acgaccgacg gcgctcccga gctcccggag ccggcgtgga ccgcgatgtc
       61 ggtgaactgg gtgagctgcc cgacgagcgc ctgctccgac gagcgctcca cgtccacgac
      121 gaagtgcgac tgcagctcgg cgccgaggtc ctcggggatg tcctccgcac gcacgatcgc
      181 caccgtcgtc cagccgtcga ccaggaaggc cccgaggccg acctggagcg acacggtcgc
      241 ctcctcgggc atcgcaccga ggagcgcatc ctcgtccacc gcgtgcaacc acccggtcgc
      301 gggtgccacc acggtcgcgg tggacggccc gggtgcgggc gccgcgtcgt cctcgtgtcc
      361 gcccgcgccg cggccggctg cccggatgct gtcgatcacg ccgtgggcca cctcggagac
      421 gagcacgctc ggccgggtcg agcgcacggc gtgctcgatc gccacgagca gcgcagcgat
      481 ggcggcgaca ccgagcgcgg ctccgagcac ggtggtgatc gtcggcgccg aggtgtcctc
      541 cgacgacggg atggcagcag cggcaacggc ggtgtaggcg agcgccccga gcgtcacggt
      601 gatgaccagg cgctggaagc gaccgtggag ccggccggcg acgatggtgg gcgggacggc
      661 agaggctgcg agctgagcgg ccagcggcag catccagaag gcgaacgccc cggcagtgac
      721 gagcgcggag aacgtggcgg cgagcagtga cagcgcgttc gtcgtcggga tctccacgcc
      781 caggtcgacg acccggtctg cgaggagcgc cgccgcggcg acggtgacgc cgagggcgat
      841 gccggcgagc acctggaggt ggtcgcggtg acgtcgcacg aagtggtgca tgttcggcca
      901 gtacccgatc caccggtggt tcaccatggc cgggagtggc cgccgaggcc ccggtcggct
      961 caggtcaggt gctggacgag gcccgggagg tcgcgttcgc cttcgggcgt ctgtccgccg
     1021 agctcgagga accgctccat gcgccgctgc gactcggggt cggcgagcgt gcggtcgaag
     1081 cggcgagcct cctccagcag gccggcggtc gggtccggca tggcggccag gaccgccgcc
     1141 ttcgcctcgc gcaccgcgac cggcggcgag gccgcgatgc ggtcggccag gtcgtccacg
     1201 aaggggtcga gggcgtcggc ctccagcgcc cggttcacga gtccccacgc ctctgcggtg
     1261 cgggcgtcga tgtcaccgga cccgaggacc atctcgagcg cccgaccgcg accgaggagg
     1321 tggggcagcc gctgggtgcc gctgccgccg ggcaggatgc cgagcgcgac ctccggctgg
     1381 ccgaacaccg ctcgatccag cgcagcgaag cgcatgtcga acgacgacac gacctcacaa
     1441 ccaccgccgc ggacgatccc ggcgatggac gcgatcgtgg cgcacggcat ggtgcgccac
     1501 cgctcgacca tcacgtggaa cggcccgagc tcgtcgcgat cctccgcacc cccgtcgcgt
     1561 ggcagctcga ggatggccgc cacgtcgaag tgcgcgatga agaagtccgg gttggcggag
     1621 ctgacgacca ccaccgtgac gtcgtcggcc acctcgaggg cctccgtcag ccggagcaag
     1681 gagacgagga gcgggacgtc gagcaggttg atgtcgccgt ggtcgatggt gacgtcgacc
     1741 cgggagccgc gctgggcgac ccgcaggccg gtgagctcgg gcaggtcagc ggcgaaggac
     1801 atggccggag ggtacgcccg gatgcggagg tgtcaggcgg gtggcgagct gcggctggga
     1861 gcggagatcc gcaggagttc ctgtcagcac cccttcgtac catttgtccc atggagtcgt
     1921 ccgagatcgg gatggtcgtt gactccctcg ccgaggggat caccgacctc cacgcggccg
     1981 acctcagccg gctcaccgcc cgcgagctgc tccagctcgc cggcgcgctc gaggtcgtga
     2041 gccgacggct cgaccacgcg agggacaccc tcgccggcca catcgatgcc accggcgccc
     2101 acggcgtcga tggccacaag agcgccaagg ccgccctcgt ccacatcaac cgggtgcccg
     2161 gtgccgaggc gcacggcagc atccagactg cccgcgccct gcgccagctg cccgaggtcg
     2221 acgccgccta cgctgccggg aagatcccca ccgcatccgt gcgcgccatc gcacgcgtcg
     2281 cgtccaaccc ccgggtcgag tgcttcctcg acgacctcgt cgaccgcatc ttcgccgagc
     2341 aggcctccga ggccacccac gacgatctcg tctccttcgt ccgcgactgg gagcgcttcg
     2401 ccgatgccga cggcgccgac caggcccggg agcacgccca ccgccggcgc aacgcgtcgc
     2461 tggtccgcaa cgacctggcg ggcagctggc acctgcgggg cgacttcgga gatctgcacg
     2521 gcgcgtccat ggccgaggtg ctggccgcct acgagcacgc cgagtggctc gccgaccggg
     2581 aggtcgccgt ggccgagcac ggcccgggcg ccacgcccga ccagttcccc cgcacgcccg
     2641 cgcagcgtcg ggccgacgcc atgctcgcca tcttccggcg agccgcggcg acgcctgccg
     2701 acgcccagtc gccggagccc ctcgtcaaca tcgtcatcga ccaggagacg ctcgagaccg
     2761 agctcgcacg agccgccgga gcgcccgtcg accacgacgc atcccgtcac tccggccgga
     2821 tctgccgtac caccagcggc caccccatcc accccgccga tgcggtcgcc gcgatcctgg
     2881 tcggccacgt ccgccgcgtc gtggtcgatg ccgcatccaa cgtcatcgac ctcggccgcc
     2941 gccggcgctg cttcaccggc agcagccggg acgccgccct cctccagacc gcaatccgcg
     3001 accggggcgg caccacctgc ttctgggccg actgcaacag cccaccgcag tggatgcagg
     3061 tcgatcacga ccagacctgg cgagccctcg gcccgtcgga catcgccaac agccagacgg
     3121 cgtgcggcta ccacaaccgc ttgaaggaga ccggctaccg gcccgtccgc ggccccgacg
     3181 gcacctacac gtaccggcgc ccggacggca ccgagatcac cccgccggtc tgaccgcgcc
     3241 gtcaccacct ggaggacgtc gggtcaccgc tcccggccga atcgggaccg atggccctaa
     3301 cgacggcaca cgccacccat cgatgctcgt cggagacgtg ggggtgcacc atggccgacg
     3361 acgagcagga gcgaggaccg gcgagggagc aggacggccc gccgaagcgg taccagacct
     3421 ggaacgggga accgtgccct cggtacgtgc gcatcgacgg caccgtggtg ggttcgggcg
     3481 actgcctcgg gtgcgggacc tgcctgctgg gatccggcct cgtctgaccg acgccccggt
     3541 gcggtgctcg acggacgcca ggggtgctac gtcggctgct ggacgatgcc cttcgtcgga
     3601 aggaacggcg actccgcgcc cgcaacctcg cgaaggtcga gtacgtcgtg gaccggtgca
     3661 gcgacggcga tggtctccac ctcctccacg ccgtcgatcc ggcatccgaa agcctgctcg
     3721 acctgttggc gtgtcctgcg gctcgcgggc tcgctggccg tgcacgcggc cttggtgtcc
     3781 caggtgacga tggagagacc gttgcccgac ggtgcgtcga ccagccagca catcgaaagg
     3841 aaacccgcac ggtcttcgta acggcgaagc gcagtgcgga acaggacatt gtccggatcg
     3901 tccaggtccg ggcccagacc ctcgacgaag gtga
//
LOCUS       DATISU010000243         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_851823, whole genome shotgun sequence.
ACCESSION   DATISU010000243 DATISU010000000
VERSION     DATISU010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851823"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1801
                     /gene="rho"
                     /locus_tag="VLR27_09750"
     CDS             47..1801
                     /gene="rho"
                     /locus_tag="VLR27_09750"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HSP03774.1"
                     /translation="MSVDANALERETLERKDRDELQKIVGALGGKATTRTRKADLIDQ
                     ILELTGVSPAAEDQEQPSTDEASEPEAKAEPEPAAEPSDRAEEAPAEKPAPERPRTGV
                     AARRAGGKAGDGQDDTKGQGAKAQDTKAQDTKAQDSKSQDAKQGKGGQDDKQAKGGQD
                     DKRGQDDTRGQDQGGDNRDDSGDDTDGSGKRRRRRGRNRDGAPGQEEYSGDPVPVVGV
                     LDLRDDGYGFLRVDGLLPSKDDCYVSVKQVRQFGLRKGDVVAGGSRPAFRNEKNPAIL
                     HIDSVNEVALEPGTERPHFEDLTAVFPTERIALTLDDEADDRTTRAIELLAPIGKGSR
                     VLVLAPPRTGATTLLQSLVRSVEVNEPDATLVVVLLDERPEEITEMERLVTTGTVYGS
                     PFDKPADEHVAVLELALARAQRMAEAGDDVVLLVDGLTRLARAAHASSPGTGRSLGGL
                     DVSAVQAAKRCFGTGRNLEEGGSVTLVATAKVDTGSDVDAAVVDAVMGAASTHIRLDR
                     AAAERRAFPAIDVGATGTRHEERLVGDDGAEARDALRRSLEELPADRASGHSDALDGL
                     LERLRSTGSNDELLEQNR"
     gene            1798..1995
                     /gene="rpmE"
                     /locus_tag="VLR27_09755"
                     /pseudo
     CDS             1798..1995
                     /gene="rpmE"
                     /locus_tag="VLR27_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            2270..>2734
                     /locus_tag="VLR27_09760"
     CDS             2270..>2734
                     /locus_tag="VLR27_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03775.1"
                     /translation="MAADGRAFVLVEGDHSLGAALAWARRAGATEVHVIADEAPGVVA
                     RQAAEFATDVTVWRTLGRELLPAEPSPVEPGEGPPDAPDLVALMEAEGLDVVVEGDRL
                     AGEYRGLEVVRVVRALDDGAPILEVGVGKYDRALTAMMHGDLDGRASIARVVE"
BASE COUNT          430 a         1037 c          980 g          287 t
ORIGIN      
        1 ccggcgcgga cctccatccg aacacgacga aagcacgacg agcacgatga gcgtggatgc
       61 caacgccctc gagcgggaga ccctcgaacg caaggaccgc gacgagctgc agaagatcgt
      121 cggcgcgctg ggcggcaagg caacgacccg cacgcgcaag gccgacctga tcgaccagat
      181 cctcgagctg acgggcgtct cgcccgccgc cgaggaccag gagcagccgt ccacggacga
      241 ggccagcgag ccggaggcga aggccgagcc cgagcccgcc gccgagccct ccgaccgcgc
      301 cgaggaggcg ccggcggaga agccggcccc ggagcgaccc cgcacgggcg tcgccgctcg
      361 ccgagccggc gggaaggccg gcgacggcca ggacgacacc aagggccagg gcgccaaggc
      421 ccaggacacg aaggcccagg acacgaaggc ccaggacagc aagtcccagg acgccaagca
      481 gggcaagggc ggccaggacg acaagcaggc caagggcggc caggacgaca agcgcggtca
      541 ggacgacacg cgtggccagg accagggcgg cgacaaccgc gacgactccg gcgacgacac
      601 cgacgggagc ggcaagcgcc gccgccgccg gggccgcaac cgtgacggcg cccccggcca
      661 ggaggagtac agcggggatc ccgtccccgt cgtcggcgtg ctcgacctgc gcgacgacgg
      721 ctacggcttc ctccgggtcg acggcctgct gccgtccaag gacgactgct acgtgtcggt
      781 caagcaggtg cgccagttcg gcctgcgcaa gggcgacgtg gtcgccggcg gctcccgtcc
      841 ggcgttccgc aacgagaaga acccggcgat cctccacatc gacagcgtga acgaggtcgc
      901 cctcgagccc ggcaccgagc gtccccactt cgaggacctc accgccgtgt tccccacgga
      961 gcgcatcgcg ctcacgctcg acgacgaggc cgacgatcgc accacccgtg ccatcgagct
     1021 gctcgcgccg atcggcaagg gcagccgcgt gctggtcctc gccccgcccc gcaccggggc
     1081 cacgacgctg ctgcagtcgt tggtccgctc ggtcgaggtc aacgagcccg acgcgaccct
     1141 ggtcgtcgtg ctgctcgacg agcgccccga ggagatcacc gagatggagc gcctggtcac
     1201 caccggcacc gtctacggct cgcccttcga caagccggcc gacgagcacg tcgccgtgct
     1261 cgagctcgcc ctcgcccgtg cccagcgcat ggccgaggcc ggcgacgacg tcgtgctcct
     1321 ggtcgacggc ctcacccgcc tcgcccgagc cgcccacgcg tcctcgccgg gcaccgggcg
     1381 ctcgctcggc gggctcgacg tgtccgccgt gcaggccgcc aagcgctgct tcggcaccgg
     1441 tcggaacctc gaggagggcg gctccgtcac cctcgtggcc acggcgaagg tcgacaccgg
     1501 ctccgacgtg gacgccgccg tggtcgacgc cgtcatgggc gcggccagca cccacatccg
     1561 cctcgaccgg gccgcagccg agcgccgggc cttcccggcc atcgacgtgg gcgccaccgg
     1621 cacccgccac gaggagcggc tcgtggggga cgacggggct gaggcacggg acgccctgcg
     1681 ccgtagcctg gaggagttgc ccgctgaccg ggcgagcggt cacagtgatg cgctggacgg
     1741 cctgctcgaa cgccttcgga gcaccggatc gaacgacgag ctgctggagc agaaccgatg
     1801 aagaccgaca tccatcccga gtacaagacc gtcacggtga cctgctcgtg cggcaacgcc
     1861 ttcgagaccc gctccacggg cgaggacctg cgcgtcgagc tctgcagcga gtgccacccc
     1921 ttctacaccg gcaagcagaa gctggtggac acgggtggtc gcatcgaccg cttcgagcgc
     1981 cgctacggcc gccgcaacaa gggccccgcg gccgccctgg gcgacgccgc tgacgccgag
     2041 gctgctgctg ccgccgacgc cgaagctgcc gacgccgaga gcgccgactc cgagtcctga
     2101 ggctgcggtc ctgagcgacc gggcccacct cccagacgaa ccgacccccg ccgaggccgc
     2161 gcaggccgtc gagcgccagg cggcgcgcaa cgccgtcaag ctcgccgccc tcgtgcgcga
     2221 ccggtggggt gtcgacgcgc cccggtccaa cgtcggcacc ggggcggcta tggccgccga
     2281 cgggcgcgcc ttcgtcctcg tcgagggcga ccacagcctc ggcgccgccc tggcctgggc
     2341 gcggcgcgcc ggtgccaccg aggtccacgt catcgccgac gaggctcccg gcgtggttgc
     2401 tcgccaggcg gcggagttcg ccaccgacgt cacggtctgg cgcacgttgg gccgtgagct
     2461 cctcccggcc gagccgtcac cggtggagcc cggggagggt ccgcccgacg cccccgacct
     2521 ggtggcgctg atggaggccg agggcctcga cgtggtggtc gagggcgatc gcctggccgg
     2581 ggagtaccga ggtctcgagg tcgtgcgggt cgtgcgggcg ctggacgacg gcgccccgat
     2641 cctcgaggtc ggcgtcggca agtacgaccg ggccctcacc gcgatgatgc acggcgacct
     2701 cgacggccgg gcgtccatcg ctcgggtcgt cgag
//
LOCUS       DATISU010000244         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_804223, whole genome shotgun sequence.
ACCESSION   DATISU010000244 DATISU010000000
VERSION     DATISU010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804223"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..517)
                     /locus_tag="VLR27_09765"
     CDS             complement(107..517)
                     /locus_tag="VLR27_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03776.1"
                     /translation="MDPRHPSKHGNDVVEPATQAEVNRRRDAQAGQTDEVLGTPTTPV
                     LPSSMGKGMFAGAGIGAVVGALLLTPLAFANILDLDFFVRLVIVWVAGAAAGATIGAV
                     FFAGAKAEMDNPENDEYLYADGSSDDRRQGPGLD"
     gene            602..>2711
                     /locus_tag="VLR27_09770"
     CDS             602..>2711
                     /locus_tag="VLR27_09770"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HSP03777.1"
                     /translation="MRALTTTDVARRPAPGTTVPSSVRFSPDGRAATYLLAEPGSLHQ
                     SLVVVDVERGTATELPAPGDAVDEAALGLEERLRRERARELAVGVTRYQWADRADRVL
                     VPMSDGLWVLDGLAAGEPDPPRLAVPAGAVDGAILDARLSADGALVGFVAGDEVHVAD
                     VEGGSPRAVTAGAAGTGRTNGLAEYVAQEEMGRPGGFWFSPDGAHIAFCEVDETHIPV
                     YRIVHQGSDEVGAGAQEDHRYPFAGAANALVRVGVVPTDGSGATSPAWLDLDASGIGA
                     DRYVARVDWIDDGAVVVQLQSRDQRRLDVVRYDLSAAHDGTVPGSLLWSEESDVWINL
                     HDVLRPLPDGALLWASERTGFRHLEVREADGSLRHVLTEGPWVVTDVVGVGAGEVWFR
                     STGDGGTASPLERHVHRVPLDASTPPEQLTTEPGVHGAVAHPETGAWVHTTSTPDAPP
                     RTVLHAGGRTHVLHDAADDPRVDELDLRAPALRTVTTEPLQDGGLETELHAAVYRPDG
                     PGPFPTVVWVYGGPHAQLVTRSWAPTVALRAQLLRQDGFCVAVVDGRGSTDRGLAFEG
                     ALRHRMGTVEVDDQVRLVETLVAEGTTDPERVGIYGWSYGGYLSALCLARRPDVFRAA
                     CAGAPVTSWDGYDTHYTERYMGTPAENPDGYRNASVMTHVEGLRDRHLLLVHGLIDEN
                     VHFRHTARLINALVRADIDHR"
BASE COUNT          347 a         1012 c         1008 g          344 t
ORIGIN      
        1 cagcggtgcg gccccaccgg cgcgctcgac ggggacgtcg tcaccgccgc aggcggcgag
       61 gaccaggcag agcgccagcg cgagacgggc cgggatgcgc aggggttcag tcgaggccgg
      121 ggccctggcg gcggtcgtcc gacgaaccat ccgcgtagag gtactcgtcg ttctccgggt
      181 tgtccatctc ggccttggcg ccggcgaaga acacggcacc gatggtggcg ccagccgcag
      241 cgccggcgac ccacacgatg accaggcgga cgaagaagtc caggtccaag atgttggcga
      301 aggcgagcgg cgtcagcagc agggcaccga cgacggcacc gatgccggcg ccggcgaaca
      361 tccccttgcc catcgacgag gggagcaccg gggtggtggg ggtgccgagg acctcgtcgg
      421 tctggccggc ctgggcatcg cgtcgacggt tcacctcggc ctgggtggcg ggctcgacga
      481 cgtcgttgcc gtgcttgctc gggtggcggg ggtccatcgg gggtctcctg gctcgcgggt
      541 tgccggttgc cgcgtccgta ccccatgacc agccggggaa gacgcccgta gggtcgggct
      601 gatgcgtgcg ctgacgacca ccgacgtggc ccgccggccc gcgcccggca ccaccgttcc
      661 gagctccgtc cggttctcgc ccgatggtcg tgccgccacc tacctgctcg ccgaaccggg
      721 gagcctccac cagtccctcg tcgtcgtcga cgtcgagcgc ggcacggcga cggagctgcc
      781 cgcgccgggc gatgcggtcg acgaggcggc gctcggcctc gaggagcgcc tccgtcgcga
      841 gcgggcccgg gagctggccg tcggggtgac ccgctaccag tgggccgacc gagccgaccg
      901 ggtgctggtg ccgatgtccg acgggctctg ggtgctcgac ggcctcgccg cgggcgaacc
      961 cgacccaccc cgcctggcgg tccctgccgg cgccgtcgac ggcgccatcc tcgacgcccg
     1021 actctcggcg gatggcgccc tggtcggctt cgtggccggc gacgaggtcc acgtcgccga
     1081 cgtcgagggc ggatcaccgc gggcggtcac cgccggcgcc gcgggcaccg ggcgcacgaa
     1141 cgggctggcc gagtacgtgg cccaggagga gatgggccgg cccggcggct tctggttctc
     1201 gcccgacggc gcgcacatcg cgttctgcga ggtcgacgag acccacatcc cggtgtaccg
     1261 catcgtccac cagggcagcg acgaggtcgg tgcgggggcg caggaggacc accggtaccc
     1321 cttcgccggt gcggcgaacg cgctggtgcg ggtgggtgtc gtgcccaccg acggcagcgg
     1381 ggcgacgtcg ccggcctggc tcgacctcga cgccagcggc atcggcgccg accgctacgt
     1441 ggcccgcgtc gactggatcg acgacggcgc cgtggtcgtg cagctgcagt cccgcgacca
     1501 gcgacggctc gacgtcgtcc gctacgacct gtcggccgcg cacgacggca ccgtgcccgg
     1561 ctcgctgctc tggtccgagg agtccgacgt gtggatcaac ctgcacgacg tcctccggcc
     1621 gctgccggac ggcgcgctgc tgtgggccag cgagcggacc gggttccgcc acctcgaggt
     1681 gcgcgaggcc gacggcagcc tccgccacgt cctgaccgag ggtccgtggg tggtcaccga
     1741 cgtcgtgggc gtcggggcgg gcgaggtgtg gttccgcagc accggtgacg gtgggacggc
     1801 atcgccgctg gagcgccacg tccaccgggt gcccctcgac gccagcaccc cgcccgagca
     1861 gctcaccacc gaacccgggg tgcacggcgc ggtcgcccac ccggagaccg gcgcctgggt
     1921 gcacaccacg tcgaccccgg atgcgccccc tcggaccgtg ctgcacgccg gtggccggac
     1981 ccacgtgctg cacgacgccg ctgacgaccc ccgggtcgac gagctcgacc tgcgcgcgcc
     2041 ggcgctgcgc accgtgacca ccgagcccct ccaggacggt ggcctggaga ccgagctgca
     2101 cgccgccgtg taccggcccg acggaccggg tccgttcccc acggtggtgt gggtctacgg
     2161 cggtccgcac gcccagctgg tgacccggtc gtgggcgccg acggtcgccc tgcgggccca
     2221 gctcctgcgc caggacggct tctgcgtggc cgtcgtcgac gggcgggggt ccaccgaccg
     2281 tggcctggcc ttcgaaggcg ccctgcgcca ccgcatgggc accgtggagg tggacgacca
     2341 ggtgcgcctg gtcgagacgc tcgtcgccga gggcaccacc gaccccgagc gggtcggcat
     2401 ctacggctgg agctacggcg gctacctgag cgccctgtgc ctcgcccgcc gtcccgacgt
     2461 gttccgggcg gcctgcgccg gcgcacccgt gacctcctgg gacggctacg acacgcacta
     2521 caccgagcgc tacatgggca cgccggccga gaaccccgac ggctaccgga acgcctcggt
     2581 gatgacccac gtcgagggcc tgcgcgaccg gcacctgctc ctcgtgcacg ggctgatcga
     2641 cgagaacgtg cacttccgcc acaccgcccg gctgatcaac gcgctggtgc gggccgacat
     2701 cgaccaccgg c
//
LOCUS       DATISU010000245         6342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_338947, whole genome shotgun sequence.
ACCESSION   DATISU010000245 DATISU010000000
VERSION     DATISU010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338947"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VLR27_09775"
     CDS             complement(<1..1045)
                     /locus_tag="VLR27_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03778.1"
                     /translation="MRTTTTKLLALGTAAALGLAACGGSDEETVPAVSGEARPVQETE
                     PAEGEQTDATAEEAEAPEVETSTDDAASTLRAELTALLQEHVHLTAIAVDAVVEDGAD
                     APGAQSAVQALDESSTALGERIAGIPSVEDPEAFLEPWQAHIDAYLDLAAARADEDDD
                     AAAEATTALEEAPQPLADFFEEISDGELSADELFGELETHVTMVTDAIDAHAGGDTEA
                     ADLWRDAALQMDSLAADLAEGIVATHPDELAGDPLSVPAETRATLTSALVEHTYLTLL
                     AAGEAADAGGATDDPAVQAAIMTLDGSADDLANAANGVQGMDGRDAFLDAWRPFLAAA
                     TDFAAATASGDAAA"
     gene            complement(1216..2508)
                     /locus_tag="VLR27_09780"
     CDS             complement(1216..2508)
                     /locus_tag="VLR27_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03779.1"
                     /translation="MRPTTKKLLAISLAGSMALAACGGDDDSTVAQSGDPAPVEETTS
                     TTEAMEDDMDMSEGETAGTETGAATLRAGMTSLLQEHVYLAGIAIETALDAGGDMEAP
                     AVQAAVETLDANSIALSEAVGSVAGDENAEAFLGLWREHIGFFVDYTLGQATGDQAAS
                     DQALTDLEGYQQAAGDFFEEITAGELPAEALVESLDVHIETLTAAIDALVAGDAQAFD
                     LLQAAADHMPMAATAISGAIVAAVPDMFDGEVDSAPAETRAVLTDLLQEHVYLAGIAI
                     EQAVEAGGDMEAPAVQAAVATLDANSVELAEVVGSVSTPEDQEAFLGLWREHIGFFVD
                     YTLGQATGDQAAADQALTDLEGYQQAAGDFFEEITAGELPAEALVESLDEHVATLTTA
                     IDSLIAGEPSVFDDLRAAGQHMPMAAAALAGAIVAATS"
     gene            2661..3020
                     /locus_tag="VLR27_09785"
     CDS             2661..3020
                     /locus_tag="VLR27_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HSP03780.1"
                     /translation="MTPRRALLPSLVLAAVLLSACGDDDDSTAADAPAADAVVTMEES
                     RFAPDSLDVAPGTEVTFENLDPYAHTVTAAEGSTAEFDSGQLGEGDTFVQTFDDAGTY
                     EYFCEIHPTMRAEVVVG"
     gene            complement(3024..3560)
                     /locus_tag="VLR27_09790"
     CDS             complement(3024..3560)
                     /locus_tag="VLR27_09790"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HSP03781.1"
                     /translation="MPAEPAAIPDGANIVLTGFMGTGKSTVGRLVAERLGRRFVDTDA
                     LIESRHGPIPRLFAEHGEDHFRRLERDVASELAATSGLVVATGGRTMLDPANQATLGA
                     NGTVVCLAASVEQLVLRLQDEAEDRPLLQGDDPAGRITALLAEREAGYARFPQVVTDG
                     RTPAEVADAVIALVADAP"
     gene            3614..4069
                     /gene="aroQ"
                     /locus_tag="VLR27_09795"
     CDS             3614..4069
                     /gene="aroQ"
                     /locus_tag="VLR27_09795"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HSP03782.1"
                     /translation="MTRRVLVLNGPNLNLLGAREPEVYGADTLDVVLERLERHVEGRD
                     VELRAVQSNVEGELVDALHEARTWADGVVFNPGAFTHYSIALRDAISGTGLAVVETHL
                     SNVHAREEFRHRSVLAAVCLGVVAGFGADSYLLALDGLLRHLEQRGLST"
     gene            complement(4066..4281)
                     /locus_tag="VLR27_09800"
     CDS             complement(4066..4281)
                     /locus_tag="VLR27_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03783.1"
                     /translation="MSTTPEADRTTIARIAADRAQALRDHAASITSEALAPLQAALHI
                     RAGELEMAAAALGDPDARPSRHLQAIA"
     gene            complement(4358..4990)
                     /locus_tag="VLR27_09805"
     CDS             complement(4358..4990)
                     /locus_tag="VLR27_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03784.1"
                     /translation="MPSPEAMAAALVRAHAEPTDGLPDGRRLVDYAESSRTGDWSLRS
                     ALVRFAQPEPTRASAVLELIRRTDGALKPHRRQLEAGDAPTHPGLEPGTFVDDGGQLV
                     VDPDADRVIDAPAADLARAVLRLPEGDAVVASYRDAADGDPTFDAIPLLVVALELDAI
                     AEALVAWACVHDGPPPVAVVDERAAAAFTRLAALDVPRETGGRPPGRGRS"
     gene            complement(5073..>6342)
                     /locus_tag="VLR27_09810"
     CDS             complement(5073..>6342)
                     /locus_tag="VLR27_09810"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HSP03785.1"
                     /translation="GSSDEGGASTSALATLTEFAINPEALVVSGSGTLDVRNDGSQVH
                     NLVVVDEDVRTADLNGGESESLDISALDEGEYEVICDIAGHADAGMRGTMTVGDVPAG
                     EAASGGDHGGGHGAEMTQEEADALDQAMIDSMMAFPAETEGSGNEPLEPVEIMEDGTK
                     RFELTAEIIEWEVSPGEFVEAWAYNGMVPGPYIKVDLGDNLRFDVTNELPLGTDVHWH
                     GVRTPNSMDGVAPYTQDVIKNGETFTYEFEAVKKAVGIYHAHIHGQVAVPNGMLGIIQ
                     IGDVDLPRGRTISGVEIPADLEIAQEIPMVLNDAGSIGLSLNGKSFPATEPIVTNNGD
                     WVLVHYANEGLQVHPMHQHQFSQLVVAKDGIALDNPYFADTVNVAPGERYSVLINTDD
                     PGTWVFHCHILTHVEREEGMFGMVTAMVVN"
BASE COUNT          773 a         2150 c         2465 g          954 t
ORIGIN      
        1 ctgccgccgc atcgccggat gcggtggccg cggcgaagtc ggtggcggcg gcgaggaagg
       61 ggcgccaggc gtcgaggaac gcgtcgcggc cgtccatccc ctgcacgccg ttggcggcgt
      121 tggccaggtc gtcggcgctg ccgtcgaggg tcatgatcgc ggcctgcacg gcggggtcgt
      181 cggtcgcgcc gccggcatcg gcggcctccc cggcggccaa cagggtgagg taggtgtgct
      241 ccaccagcgc actggtgagc gtggcgcgcg tctcggcggg cacgctcagc ggatcgccgg
      301 ccagctcgtc ggggtgggtg gccacgatgc cctcggcgag gtccgcggcg agggagtcca
      361 tctgcagggc ggcgtcgcgc cagaggtccg cggcctcggt gtcgccgccg gcgtgggcgt
      421 cgatcgcgtc cgtcaccatc gtcacgtggg tctcgagctc accgaacagc tcgtcggcgc
      481 tgagctcccc gtcgctgatc tcctcgaaga agtcggccag cggctggggc gcctcctcga
      541 gggcggtggt ggcctcggcg gccgcgtcgt cgtcctcgtc ggcgcgtgcc gctgccaggt
      601 ccaggtaggc gtcgatgtgc gcctgccacg gctcgaggaa ggcctcgggg tcctcgaccg
      661 acgggatgcc ggcgatgcgc tcgcccagtg cggtcgagct ctcgtcgagc gcctggacgg
      721 ccgactgggc cccgggtgcg tcggctccgt cctccacgac ggcgtcgacg gcgatggcgg
      781 tgaggtggac gtgctcctgg agcagcgcgg tgagctcggc ccgcagggtg gatgcagcgt
      841 cgtcggtgct ggtctcgacc tccggggcct cggcctcctc ggcggtcgcg tcggtctgct
      901 cgccctcggc gggttcggtc tcctggaccg ggcgggcctc gcccgacacg gcggggacgg
      961 tctcctcgtc ggagccgccg caggcggcca gaccgagcgc agcagcggtg ccgagcgcga
     1021 ggagcttggt ggtggtcgtt cgcacggtgc tctccagcgg gtggggggtg ggggcgcgcc
     1081 cgtgcgggcg cggatcggtc cttctcccac cggcacagat cggtagaact cgggcgcgac
     1141 gacggcccgc cccgcgagcg ggacgggccg aggccgtgct ggtgagcggg agcggtcgtg
     1201 ccgctcagcc ggaggtcagg acgtggcggc gacgatcgcc ccggccaggg cggcagcagc
     1261 catgggcatg tgctgcccgg cggcgcggag gtcgtcgaag accgacggct cgccggcgat
     1321 cagcgagtcg atcgcggtcg tcagcgtggc gacgtgctcg tcgaggctct cgacgagggc
     1381 ctcggcgggg agctcaccgg cggtgatctc ctcgaagaag tcgccggcgg cctgctggta
     1441 gccctcgagg tcggtgagcg cctggtccgc agcggcctgg tcaccggtgg cctggccgag
     1501 cgtgtagtcg acgaagaagc cgatgtgctc gcgccacagg ccgaggaacg cctcctggtc
     1561 ctcgggcgtg gacaccgagc ccacgacctc ggccagctcg acggagttgg cgtccagcgt
     1621 ggcgaccgcg gcctgcacgg ccggggcctc catgtcgccg ccggcctcga cggcctgctc
     1681 gatggcgatg ccggccaggt agacgtgctc ctggagcagg tcggtcagca cggcccgggt
     1741 ctcggcgggg gcgctgtcga cctcgccgtc gaacatgtcc gggacggcgg cgacgatggc
     1801 accggagatc gcggtggcgg ccatcggcat gtggtcggcg gcggcctgga gcaggtcgaa
     1861 cgcctgggcg tcgccggcga cgagggcgtc gatggcggcg gtcagggtct cgatgtggac
     1921 gtcgaggctc tcgacgaggg cctcggcggg gagctcgccg gcggtgatct cctcgaagaa
     1981 gtcgccggcg gcctgctggt agccctcgag gtcggtcagg gcctggtcgg atgcggcctg
     2041 atcgccggtg gcctggccga gcgtgtagtc gacgaagaag ccgatgtgct cgcgccacag
     2101 gccgaggaag gcctcggcgt tctcgtcacc ggcgacggag ccgacggctt cgctcagggc
     2161 gatggagttg gcgtcgaggg tctcgaccgc ggcctggacg gccggggcct ccatgtcgcc
     2221 gccggcgtcg agggcggtct cgatcgcgat gccggccagg tacacgtgct cctgcaggag
     2281 cgaggtcatc ccggcgcgca gggtcgcggc gccggtctcg gtgccggcgg tctcgccctc
     2341 ggacatgtcc atgtcgtctt ccatcgcctc ggtcgtcgag gtggtctcct cgacgggggc
     2401 ggggtcgccg gactgggcga cggtgctgtc gtcgtcaccg ccgcaggcgg cgagcgccat
     2461 cgagccggcc aggctgatgg cgagcagctt cttcgtggtg ggtcgcatga gggatctcca
     2521 aagggggtcg cgatcggggg gaacgagtcc ggggcggcga cgtgggggcg tcgccggcgt
     2581 cccggggtct cgccccttct acgacgccac ccgggatctg gatgacgtcc gtcgggatcc
     2641 gtggttccgt agagatcgtg atgaccccac gccgagcgct cctcccgtcc ctggtcctcg
     2701 cggccgtcct cctctctgca tgcggcgatg acgacgacag caccgccgcc gacgccccgg
     2761 cggcggacgc cgtggtcacg atggaggagt cccggttcgc gcccgactcc ctcgacgtgg
     2821 cgccgggcac cgaggtcacg ttcgagaacc tcgatcccta cgcccacacg gtcacggcgg
     2881 ccgagggcag cacggccgag ttcgactcgg gccagctcgg cgagggggac accttcgtgc
     2941 agaccttcga cgacgccggc acctacgagt acttctgcga gatccacccg acgatgcggg
     3001 cggaggtcgt cgtcggctga cggctacggc gcgtcggcga ccagggcgat gaccgcgtcg
     3061 gccacctctg ccggcgtgcg cccgtccgtg acgacctggg ggaaccgggc gtagcccgcc
     3121 tcccgctcgg cgagcagggc ggtgatccgg ccggcggggt cgtcgccctg cagcagcggg
     3181 cggtcctcgg cctcgtcctg cagccgcagc acgagctgct cgaccgaggc ggccaggcac
     3241 accaccgtcc cgttggcgcc gagggtggcc tggttggcgg ggtcgagcat cgtccgccca
     3301 ccggtggcca cgacgaggcc ggaggtggcg gcgagctcgg atgccacatc gcgctcgagc
     3361 cggcggaagt ggtcctcgcc gtgctcggcg aagagtcgcg ggatgggccc gtgccgggac
     3421 tcgatcaggg cgtcggtgtc gacgaaccgg cgcccgagtc gctcggccac caggcgtccg
     3481 acggtggact tgccggtgcc catgaacccg gtgaggacga tgttcgcccc atcggggatc
     3541 gcggcgggtt cggcgggcac agggcgtcgg atggtaccgg tcgcccggag gccgccctcg
     3601 taggctcgcc ggcatgaccc gccgcgtcct cgtgctgaac gggccgaacc tgaacctgct
     3661 cggcgcccgg gagcccgagg tgtacggcgc cgacacgctg gacgtcgtgc tcgagcggct
     3721 ggagcgccat gtcgagggcc gggacgtgga gctgcgcgcc gtgcagtcca acgtcgaggg
     3781 cgagctcgtc gacgccctcc acgaggcccg cacgtgggcc gacggggtgg tgttcaaccc
     3841 cggggcgttc acccactaca gcatcgccct gcgcgacgcg atcagcggga cgggcctggc
     3901 cgtggtcgag acccacctgt cgaacgtgca cgcccgggag gagttccggc accgctccgt
     3961 gctggcggcg gtgtgcctgg gcgtcgtcgc cggattcggt gccgacagct acctgctggc
     4021 gctcgacggc ctcctccggc acctcgagca gcgaggactg tcgacctagg cgatcgcctg
     4081 gaggtggcgc gacggccggg catccgggtc gcccagggcg gcggcggcca tctcgagctc
     4141 gccggcccgg atgtgcaggg cggcctgcag cggggcgagg gcctcgctcg tgatcgaggc
     4201 agcgtggtcg cggagggcct gggcccggtc ggcggcgatg cgtgcgatgg tggtgcgatc
     4261 ggcttcgggg gtggtcgaca tggggtgcct ccggggagtg tgacgacgct ggggtgcggc
     4321 gatcggtacc cctgggtgag ccgtgacaga acctcggtca ggaccgaccc cggccgggtg
     4381 gacgcccgcc cgtctcgcgc gggacgtcga gcgcggccag gcgggtgaac gccgccgcgg
     4441 cccgctcgtc gacgacggcg accggcggcg ggccgtcgtg gacgcacgcc cacgccacca
     4501 gcgcctcggc gatcgcgtcc agctccaggg ccacgaccag cagcgggatg gcgtcgaacg
     4561 tcgggtcgcc atcggcggcg tcgcggtagg acgccacgac cgcgtcgccc tccggcaacc
     4621 ggagcacggc ccgggccagg tcggcggcgg gtgcgtcgat gacccgatca gcgtcggggt
     4681 cgaccaccag ctgcccgccg tcgtccacga acgtgccggg ctccaggccg gggtgggtcg
     4741 gcgcatcgcc tgcctccagc tgccggcggt gcggcttcag ggccccgtcg gtgcgccgga
     4801 tcagctccag caccgcgctg gcgcgcgtgg gctcgggttg ggcgaagcgc acgagcgcgc
     4861 tgcgcaggct ccagtcgccc gtacggctcg attccgcgta gtcgaccagg cgacgaccgt
     4921 cgggcagccc gtcggtgggt tcggcgtggg ctcggaccag ggcggcggcc atcgcctcgg
     4981 gggagggcac ggcgccattc aaccccggac gcgccggagg cgcaggcccc ttccggtgcc
     5041 tgcgcctccg cgcccccaca tccggtggtc gatcagttca cgaccatggc ggtgaccatg
     5101 ccgaacatgc cttcttcccg ctcgacgtgg gtgaggatgt ggcagtggaa gacccacgtg
     5161 ccggggtcgt cggtgttgat cagcaccgag taccgctcgc cgggtgccac gttgaccgtg
     5221 tcggcgaagt acgggttgtc cagcgcgatg ccgtccttgg ccacgaccag ctgggagaac
     5281 tggtgctggt gcatcgggtg cacctgcaag ccctcgttgg cgtagtgcac gaggacccag
     5341 tcgccgttgt tggtgacgat cggctcggtg gccgggaagc tcttgccgtt gagcgacagg
     5401 ccgatgctgc cggcgtcgtt caggaccatc gggatctcct gggcgatctc gagatcggcg
     5461 gggatctcga cgccgctgat cgtgcggcca cgggggaggt cgacgtcgcc gatctggatg
     5521 atgccgagca tgccgttggg caccgccacc tggccgtgga tgtgcgcgtg gtagatgccg
     5581 acggccttct tgaccgcctc gaactcgtag gtgaacgtct cgccgttctt gatcacgtcc
     5641 tgggtgtacg gcgcgacgcc atccatggag ttcggggtcc gcacgccgtg ccagtggaca
     5701 tccgtgccga ggggcagctc gttggtgacg tcgaaccgga ggttgtcgcc gagatcgacc
     5761 ttgatgtacg gaccggggac catgccgttg taggcccagg cctcgacgaa ctcgccgggg
     5821 gagacctccc actcgatgat ctcggcggtg agctcgaagc gcttcgtgcc gtcctccatg
     5881 atctcgaccg gctcgagcgg ctcgttgccc gagccctcgg tctcggccgg gaaggccatc
     5941 atcgagtcga tcatggcctg gtcgagggcg tcagcctctt cctgggtcat ctccgcgccg
     6001 tgcccgccgc cgtggtcgcc gccgctggcg gcctcgcctg cggggacgtc gcccacggtc
     6061 atcgtgccgc gcatgccggc gtcggcgtgt ccggcgatgt cgcagatgac ctcgtactcg
     6121 ccctcgtcga gggcggagat gtcgagggac tcggactcgc cgccgttgag gtcggcggtg
     6181 cgcacatcct cgtcgaccac gaccaggttg tggacctggg agccgtcgtt gcgcacgtcg
     6241 agggtgccgg agccgctcac gaccagcgcc tcggggttga tggcgaactc ggtgagcgtg
     6301 gcgagcgccg aggtggaggc accaccttcg tcgctgctgc cg
//
LOCUS       DATISU010000246        20779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_246317, whole genome shotgun sequence.
ACCESSION   DATISU010000246 DATISU010000000
VERSION     DATISU010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 20779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20779
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246317"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VLR27_09815"
     CDS             <1..266
                     /locus_tag="VLR27_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03786.1"
                     /translation="LWFKRRRYGLGWTPVTWQGWAVVGAFVVVLIAGAVPAERAGGAA
                     EAAYLVVVVVAIAALVSITLRKGPTPPRWRWGTSERDDPAEDL"
     gene            407..1552
                     /locus_tag="VLR27_09820"
     CDS             407..1552
                     /locus_tag="VLR27_09820"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HSP03787.1"
                     /translation="MTSLLEALSAVPADDLCAVVDMAGAALGATASRMLVADYALTSL
                     RELGDRGPDCPFQLIEGTVAGRCFAFDEIVVVADPPTVYVPMTDGSERLGVLELAHAS
                     WSDDVATAAEAIVQLLVLLMISKRRYTDIVHQTRRAEPLSVAAEMQWALLPPLACSTD
                     HVSASGILEPAYAIGGDTFDYALSRSGLDFAIVDAVGHGLPAVSISAVTVNGLRNARR
                     EERSLEVAYLDTDATLRSQFTRSAYATAQFGSLSSTTGELTWLNAGHPLPLLVRDATF
                     IGEVPCRPSLPMGLGGSVTEIAVAQLQPGDRVLFYTDGVTESQSPGGETFGVDRLADL
                     LVRGAADGTSPAESVRRLVATVVEYNDRRLRDDATLFMLEYHGNHTS"
     gene            complement(1562..2131)
                     /gene="rdgB"
                     /locus_tag="VLR27_09825"
     CDS             complement(1562..2131)
                     /gene="rdgB"
                     /locus_tag="VLR27_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HSP03788.1"
                     /translation="MSGGPRPVVLATGNAHKVDEIADILGPGWAVSAQDPDVEETGTT
                     FEENALLKARAVCETTGDLAIADDSGIEIDALDRRPGIHSARWTEESDWIPRVLRELD
                     GVTGEARSGRYVAAAAAVWPDGHEVVVRGVVEGHVPDAPRGSGGFGYDPIFVPVEGDG
                     RTFAEMGADEKHALSHRGRAFRALVEHLG"
     gene            complement(2128..2892)
                     /gene="rph"
                     /locus_tag="VLR27_09830"
     CDS             complement(2128..2892)
                     /gene="rph"
                     /locus_tag="VLR27_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HSP03789.1"
                     /translation="MSTDTETTRGDGRAPGELRPVTIERDPTVMAEGSCLISFGQTKV
                     LCTASVEDDVPRWMKGRGSGWVTAEYSMLPGSSPERVGREAAKGKQSGRTQEIQRLIG
                     RSLRSVCDMRLLGERQVTVDCDVLQADGGTRTAAITGGYLALHDALARRVAAGDIAEH
                     PLTAFCAAISVGVVKGVPVCDLPYPEDSTADVDMNVVMTSEGSFVEVQGTAEGAAFGR
                     PVLDQLLDLAEQGIGQLVERQRAYVAEAPAPRPVQR"
     gene            complement(2903..3664)
                     /locus_tag="VLR27_09835"
     CDS             complement(2903..3664)
                     /locus_tag="VLR27_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSP03790.1"
                     /translation="MNAPPGLSVTILGCSGTYAGAGGACSGYLLRTETTNLWMDCGAG
                     SLGNIQRHVPLLELDGIVVSHSHPDHWLELPVLLNALRYGVGAPDLGMPLLWTARTAE
                     QFAAIAAEPAPTFAPQVIDERSTATIGDIELSFSRTDHPVETLAIRAEHGGRSIVYSA
                     DTGDAWRLASLGPDIGRSIDLAIVEATLDEADADVVQHLTASQAGRQAAEADVATLVL
                     THLAPGSDPEARRSDAARVYDGPIEVAEIDAIHRA"
     gene            complement(3661..4470)
                     /gene="murI"
                     /locus_tag="VLR27_09840"
     CDS             complement(3661..4470)
                     /gene="murI"
                     /locus_tag="VLR27_09840"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HSP03791.1"
                     /translation="MGDDRPIGMFDSGFGGLTVARALIDLAPGEDLVYVGDTGRYPYG
                     PRPLEEVRGFAHQISRWLVEVHDVKVVVVACNTATAAALDELQAALPVPVVGVIEPGV
                     ASLVLATRNQRVGVIGTVGTISSGAYQREVAAAAPHLELTCAACPGFVEFVERGETDS
                     DQVHVLAERLLAPVVEAGVDALLLGCTHYPVLARTLSDVLGRDVVLVSSADETAFRVR
                     AMLESSWAPADRGGRGRQQWFSSGDVEVFRALGARLLGPELDVASAVRWER"
     gene            complement(4474..5370)
                     /locus_tag="VLR27_09845"
     CDS             complement(4474..5370)
                     /locus_tag="VLR27_09845"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HSP03792.1"
                     /translation="MVADPATTVTGPAVSSLLRQVPTGEVELDAVVREGLTATDWLVA
                     GATFVGFVVAAMVLRRVVERLVRRTDPEGAIARFSARLVRNLVVLVGVLYALTALGVR
                     IGPLVGALGVTGIAIAFAVTAILENVFASVMLKTRRPIRVGDQVWTNDRAGTVTEINF
                     RAVVLDSFDGERVYIPSSMVMDDVIVNHTATPTRRTVLPVGVAYGTDLHRAQDVLLAA
                     VTGVDGVIPAPAPQAWVTTFGESSIDFDVRFWTASDIATVFRVRSGVAMAIKDAFDDE
                     GIEIPFPQRVVTMVADEPGDQT"
     gene            5435..7030
                     /locus_tag="VLR27_09850"
     CDS             5435..7030
                     /locus_tag="VLR27_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HSP03793.1"
                     /translation="MGDQVTDAGWWSDAVGYEVYLRSFADSDGDGIGDLPGLTSRLDH
                     LADLGIDIVWVTPFYPSPMADFGYDVADHTGVDPRFGTIEDLDRLVAAVHERGMRLLI
                     DLVPNHTSDQHEWFRSALTGPGSPHRDFYIWRDPAPDGGPPNNWRSHFGGPAWTFDQA
                     SGQWFCHLFLPEQPDLNWANPEVRAAFRDIITHWLDRGVDGFRIDVAHSLLKHPSFAD
                     NPVLAEVGADASAGEAFQAMRHDHDLDQPETLDIYREWHRLARAHDAVLLGEVYLDSA
                     EAVGAYVADGVLDLSFFFPVLHTTWDPDVLRDVVTATLTHGRGRFAWVHSSHDDRRAP
                     TRFGGGEQGRRRALSFLAFTAFLPGPLFLYQGDELGLVDGEVDPDSAADPVAVRNTNP
                     DGGRDPVRTPMPWSPDAPNLGFSTGTPWLPMPANHTAADTVEAQRADPGSTLHAVRRL
                     LQLRDERLPNPEPVTLSAEGPAEVLGYRRGDVLVTLNVGSRAVTVPLQGPVELAFGNG
                     GVDLGGDAVVLEPDAVAVLDVLR"
     gene            7027..8091
                     /locus_tag="VLR27_09855"
     CDS             7027..8091
                     /locus_tag="VLR27_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSP03794.1"
                     /translation="MSPTDTPRRRTRTRTRTDGVTIEEVARRANVSVATVSRALRGLP
                     NVAPSTRSRVEQVARDLDYQVDTYASRLATGRSDTVGLAVPNINSWYFAQVAAGVEAV
                     LADAGMDVLLQSVDDGAGRTRLLSAQSSVRRRLDGLILIDVLLTPGEIRTLAADSVHV
                     VTVGQHTDEFPSVTVDNRRAALAATRHLLELGHRRIGLIGGSTHTGLPFDVPGHRRQG
                     YVEALLEAGLDYHADLEANGGFSVKGGSEAMVHLMSEARPPSAVFALSDEMAMGAIKA
                     ARDLGLRVPEDVSVVGFDDHDLAKVFELTTVRQDPEWHGATAGRLLLGLGDEPAPEVA
                     HVVGETRLLVRSTTSSPGGH"
     gene            8185..9573
                     /locus_tag="VLR27_09860"
     CDS             8185..9573
                     /locus_tag="VLR27_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSP03795.1"
                     /translation="MKRPLWRILAALFALSLIAASCAGDDDDTSAGDDSGDDSGDSGD
                     SGDGGDVEIESVDVFPLPPVGDSGEVVLTGGTFAGAFSADEDTMAVLEYMASPEYVER
                     RLEAEVGGFLSANQNVDLESYSGFEQTLVEILQNAEAARFDGSDDMVAEVGAGTFWSA
                     ATDIVSGNASVEEAFAEVESGYPPVPEIGECPAPEIGPGDGTVTICGPEVEQEQQGFQ
                     QSFDAFTEETGITVEVQGDRSAEEQIGVQVQGGNPPDVFIFPQPGLLRSFVEDCQLTP
                     LPENVVSAMQENFVEGFIAGGQVGDQYYGVPNKSDVKSLVWYSPEAFEANDYEVPADH
                     DEFVALMDQMVEDGNTPLCIGIGSDAATGWPFTDWIEDYMLRLHGPEVYDQWVNHEIP
                     FDDPQVVEVGEFVMDIWESEGYVFGGMQAAASTPFAESGLGIIEGDCMMHRQANFYAA
                     NWPDGIQVGSES"
     gene            9707..11056
                     /locus_tag="VLR27_09865"
     CDS             9707..11056
                     /locus_tag="VLR27_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSP03796.1"
                     /translation="MTAPTTAPTTGGEDPDGTPPDGAGTPRPRGATGSKADAAQQLVV
                     AGVLLAIAVAFWFIDSLSDSALMIALTGVVISIAASAGIFIGANGLASLLPGKTAEKL
                     RPWIFVGPALLFIFVTLVVPTVRTLYLSLLDNAGNELVGLEQYGDIFTSDDIFGFDGI
                     ADMFTSRLFILGLVALAVGIVLAVRNGRAIGTRTDFSSSGTTLAIAAGVTLVLFSLFT
                     SARGVIWNNLWWVFAVTALATALGLAVAVLADRVKGEAIAKALIFLPMAISFVGASII
                     WRFVYAFRPQASDQIGVLNAVWRGLGGAVLDPLQARPWNTFFLIIVLIWGQTGFAMVV
                     LSSAIKAVPDEYIEAARVDGATESQIFWRIILPQIRSTILVVVTTLIILVMKVFDIVK
                     VMTTGEFGTNVVANEMFDQAFRFGNVGLGAALASVLFLAVLPLMYINVRRVREESQL"
     gene            11053..12414
                     /locus_tag="VLR27_09870"
     CDS             11053..12414
                     /locus_tag="VLR27_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSP03797.1"
                     /translation="MSAIQSEPGRRLEPEGEDPRDHVVESELPPRGPRSRTTTIVAIV
                     SIVGGAIAFVWGMDWFLFGLSDEIATLTVGGLAVMVLGAWSAIGGALLLKRVSWAQPA
                     MVATYGVHALAITVFGLISAAASGGGLVDYLIPGVLLAAALAMVWMLRSPAVEKDFPS
                     DSGSGLGRTVALWSIVAVWSLPTIGLLLSSFRAERAVKTEGWWTIFTNPQLTMDNYQT
                     VLESGGSSAPNMFEAFLNSVAIVLPATVIPISIALFAAYAFAWMDFPGRKWLFVAVVS
                     LMAVPLQMSLIPLLQLYVGGAHIGSFTIFPDLDLNGKAVSVWLTHTGFGLPLAIFLLY
                     NYVSGLPKDIFEAARIDGADHYTIFWRLIVPLSVPALAAFGIFQFLWTWNDYLVALTF
                     LGGNADTHPLTMRLANLSGGRGQDWHVLTAAAFVTMSVPLVVFFSLQRYFVRGLLAGS
                     VKG"
     gene            complement(12482..13654)
                     /gene="ugpC"
                     /locus_tag="VLR27_09875"
     CDS             complement(12482..13654)
                     /gene="ugpC"
                     /locus_tag="VLR27_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HSP03798.1"
                     /translation="MAQVTLDKVSKVYPDGLEAVKDLDVDIQDGEFLVLVGPSGCGKS
                     TALRMVAGLEDITRGELRIAGRRVNEAAPKDRDIAMVFQNYALYPHMTVFDNIAFALK
                     LAKVSKSEIEKRVHKAAEILELTENLDRKPGNLSGGQRQRVAMGRAIVRQPAAFLMDE
                     PLSNLDAKLRVQMRAEIARIQRELGVTTLYVTHDQVEAMTMGDRVAVLKGGILQQVDT
                     PQNLYDHPDNVFVAAFIGSPSMNLYEGVLSRSDGGASLKLGSQTVSVPADVLERKPGM
                     KDHLGEKVTVGIRPEDMEDASLKPDHPRDQLIKTDVELVEALGSELMVHASIDAPKVH
                     SGDPDVNDEGTAGAAGTPLVGRFSPRSRVNRGETIEVAIDSRNLYFFDSKTGLTLR"
     gene            complement(13744..14691)
                     /locus_tag="VLR27_09880"
     CDS             complement(13744..14691)
                     /locus_tag="VLR27_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HSP03799.1"
                     /translation="MAVFDSVLDMIGNTPIVDCSALSPNPDVRILGKMEGQNPAGSVK
                     DRIALAMIEEAEADGTLTPGKTIIEPSSGNTGIALAMIARMKGYPIKIVLPENVSIER
                     RQLLEIYGAEIIMSPGAEGSNGAVVRARKLADEHPEWAFLYQYANEANPLSHYRTTGP
                     EIWADVPEITHFVAGLGTSGTLMGVGRFLKEQDPDIQVIAIEPPLGERVEGLRNLDEG
                     YIPPVFEKWGGAELLDRKRIVRPRESIEWTRRLSEIGIFSGISAGAALAGAVKVASEI
                     ESGTIVFVVCDYGWKYISTGAWTDDLDEVVERAEKIIYF"
     gene            complement(14784..15188)
                     /locus_tag="VLR27_09885"
     CDS             complement(14784..15188)
                     /locus_tag="VLR27_09885"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HSP03800.1"
                     /translation="MLRLAASAYAAIVGHAYDGLPDEACGLLLGDPARDSAPIFHPTA
                     NAARSSRVYSIGPRDYLDADDAAAEAGLAIIGVVHSHTHTDAYPSPTDIEQAPDPDWH
                     YVIVSLRDEAPVLRSYRIVDGTVTEEPVGIVR"
     gene            complement(15200..16108)
                     /locus_tag="VLR27_09890"
     CDS             complement(15200..16108)
                     /locus_tag="VLR27_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HSP03801.1"
                     /translation="MQPYPPRRPGPRPNYALRRLVAATGVLLVVLLVWNIVGAVTGDD
                     DSETALAPTTQPTTTTVPLQPPPPCTNPEQSEPTAYDRTDDWYRTLVDPMRAVPEQYE
                     PPDLVPASAAGYSAEYLIRALVVDDLASLRNALIAEGLPEVALLAGYRSIADQQRLFD
                     ARVAELGAGAAAEGTARGGHSEHHLGTTIDVRPIGQTDVDQAFGETPTGQWLAEHAWE
                     HGFVLSYPAGAEDVTCYKYEPWHFRYLGRDLAGRVQASGLTLRQYLWHWEVTGTEPGQ
                     SPSAPPPPLPTTSVPVETTLAEGEGG"
     gene            16175..16642
                     /gene="smpB"
                     /locus_tag="VLR27_09895"
     CDS             16175..16642
                     /gene="smpB"
                     /locus_tag="VLR27_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HSP03802.1"
                     /translation="MPAADTKLIAANRRARRNYDITETLEAGIVLHGSEVKSLREATV
                     QIADGFVRFEGKEAWLQGVHIAPYLHSQDHSGHDPDRHRKLLLHRRELDRLRTMVDLE
                     RVSIVPLSLYFKDGRAKVELGVGKGRKLHDKREVEAKRDADREIQRALAQRSR"
     gene            16723..17958
                     /locus_tag="VLR27_09900"
     CDS             16723..17958
                     /locus_tag="VLR27_09900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HSP03803.1"
                     /translation="MTKNVVILGGGTGGTLTANRLRKQFKKSELALTVVDQDRDHVYQ
                     PGLLFLPFGLAHGEDLVRPRDRQLKRGIDFVESGIDRVDVEGRRVVLSGGGELPYDVL
                     VIATGARLVPEETDGLTGPGWMEKVFTFYDLPGATGLEQALADFEGGRVVVNVVDMPI
                     KCPVAPLEFCFLADWYFHERGMRDRVQLTLVTPLDAAFTKPRAASMLGGMLEEKGIEL
                     VTEFNTGEVDGVAGRLVGFDEREVPFDLAVVVPVHSGAEYVGRSPGLGDALDFVETDP
                     HTLQSKAAPEIFAIGDAANLPASKAGSVSHFEGEVLVENVTRFLRGEPLDASFDGHAN
                     CFIETGFDKALLIDFNYEQEPVEGHFPGPVGLPLLKESRLNHLGKLMFQWFYWHSLLP
                     GRDIPGVKADMPTAGKHID"
     gene            17980..18297
                     /locus_tag="VLR27_09905"
     CDS             17980..18297
                     /locus_tag="VLR27_09905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HSP03804.1"
                     /translation="MSTATIAGSQVELNEEGFFVDPDQWTEEMVPELAAKEDIDELDD
                     KHWQVIRFMRQEYFAHGTGPTVRALGKTSGVSVKELYQLFPKGPAKIAARIAGIPKPK
                     GCI"
     gene            18330..18797
                     /locus_tag="VLR27_09910"
     CDS             18330..18797
                     /locus_tag="VLR27_09910"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HSP03805.1"
                     /translation="MDLSTHKVSIIVSQGSLEGIYPALIMANGARAEGMEANLFFTFF
                     GLDAIHRKRHEHIKMSTVGNPGLHMATWAGGLPGVSSVMTHYMEHKMEALDIPSIPEF
                     VEMISDTGAGLYACQASVDLFGLTKDDLIDQVEGIITVGDFYEMAVDGHIIYT"
     gene            18845..19942
                     /gene="ugpC"
                     /locus_tag="VLR27_09915"
     CDS             18845..19942
                     /gene="ugpC"
                     /locus_tag="VLR27_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HSP03806.1"
                     /translation="MSGVTFEQVTKRFGDVVAVDSVSLEIRDQEFMVLLGPSGCGKST
                     ALRMIAGLETVSAGELRIGDTTVNDVEAKDRDIAMVFQSYALYPHMTVRENIEVPLKT
                     RSYDVDGDKRKLRKPERADRVAEAARILGLSELLDRKPGALSGGQRQRVALARAIVRR
                     PEVFLMDEPLSNLDAKLRAQTRVELVELHRQLGTTIVYVTHDQVEAMTMADRIAVMEA
                     GKLQQVGAPRDVYARPANLFVARFIGSPPMNTVDGRVRVDGDRALVELGDGHLDIGPR
                     AGVADGDAVVVGIRPEHLRIAEDGVSAHVRAIEWLGHERHVYCDLAGTPVIVRDTDPG
                     EDHAEPGAEVMVQADPGTVHLFDPTTTERID"
     gene            19942..>20779
                     /locus_tag="VLR27_09920"
     CDS             19942..>20779
                     /locus_tag="VLR27_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSP03807.1"
                     /translation="MPVTERSRTEPPWWRRRRTREAGLAALFLAPSIVVFGAFFFYPI
                     GRIVYLGFFQQNQTRTRQRWAGTEQYVEVLTGEEFREGLGITVQYVLYTVPAGLVLGV
                     LLAVAANRRLRGIKIFHTIFASTVATSVAVASIVWLVLINPTVGYLEVDWLRDPDRAL
                     FGVALSSIWQNLGLTFIIVLAGLQAIPDEINEAATVDGMSAPRRFFTVTLPMLSPTLL
                     FLVVVLTVFAFQAFAQIDILTSGGPAGSTETLVFKIFQQQQGGSVGEGSVMAVGLFVI
                     TFL"
BASE COUNT         2981 a         7460 c         7143 g         3195 t
ORIGIN      
        1 agctctggtt caagcgccgg cggtacggcc tcggctggac cccggtcacc tggcagggat
       61 gggccgtcgt cggcgcgttc gtggtcgtcc tgatcgccgg cgccgttccg gcggagcggg
      121 ccggaggcgc cgcggaagcc gcttatctgg tggtggtcgt cgtggcgatc gccgcgctcg
      181 tctccatcac gctgcggaag ggaccgaccc cgccccggtg gcgctggggg acatcggagc
      241 gtgacgaccc cgccgaggac ctgtagcggg cgccgatccg ccggggtgac ccggcgatat
      301 gctcccgcac cagccgcaag gcccccgcaa cgacaaggaa cgcatgtctc cggcgcatcc
      361 gaacggcact gacggccgcc gcgccggatc gcttgcggac ggtccgttga cctcgctgct
      421 cgaagcgctg agcgcggtcc ccgccgacga cctctgcgcc gtggtcgaca tggccggcgc
      481 ggcgctcggc gccaccgcct cccgcatgct cgtggccgac tacgccctga cctccctgcg
      541 ggagctcggc gatcgaggtc cggactgccc gttccagctc atcgagggca ccgttgccgg
      601 ccgctgcttc gcgttcgacg agatcgtggt cgtcgccgac ccaccgaccg tgtacgtgcc
      661 catgaccgat ggcagcgagc gcctcggcgt gctcgagctc gcccacgcct cgtggtcgga
      721 cgacgtcgcc acggcagccg aagcgatcgt gcagctgctc gtgctgctca tgatcagcaa
      781 gcggcgctac accgacatcg tccaccagac ccgtcgcgcc gagccgctct ccgtcgcggc
      841 cgagatgcaa tgggcgctgc tcccgccgct ggcctgctcg acggatcacg tctcggcaag
      901 cgggatcctc gagccggcgt acgccatcgg cggcgacacc ttcgactacg ccctgtcccg
      961 ctccgggctc gacttcgcca tcgtcgatgc cgtggggcac gggctcccgg ccgtctccat
     1021 ctccgcggtc acggtgaacg gcctccggaa cgcgcgccgg gaggagcgca gcctcgaggt
     1081 cgcgtacctg gacaccgacg cgacgctgcg gtcgcagttc acccggtctg cgtacgccac
     1141 cgcgcagttc ggttctctct cctccaccac cggcgagctc acctggctca acgccggcca
     1201 tcccctcccg ctgctcgtcc gcgacgcgac gttcatcggc gaggtgccct gtcgtccatc
     1261 gctcccgatg ggcctcggcg gttcggtcac ggagatcgcc gtggcgcagc tgcagcctgg
     1321 agatcgcgtc ctcttctaca ccgacggcgt caccgagtcg cagtcccctg gtggcgagac
     1381 cttcggcgtc gatcgactgg ccgacctcct cgtccgaggt gcggccgacg ggacctcacc
     1441 ggccgagtcg gtgcggcgcc tcgtcgcgac ggtggtcgag tacaacgacc gccggctgcg
     1501 agacgacgcg accctcttca tgctcgagta ccacgggaac cacacctcct agcagcggag
     1561 gtcagccgag gtgctcgacc agcgcccgga acgcccgccc ccggtgggac agggcgtgct
     1621 tctcgtcggc gcccatctcg gcgaaggtcc ggccatcgcc ctcgacgggg acgaagatcg
     1681 ggtcgtagcc gaagccgccc gagccccgag gtgcatcggg cacgtggccc tcgaccacgc
     1741 cgcgcacgac gacctcgtga ccgtcgggcc acaccgctgc cgcggcggcc acatagcgac
     1801 cggagcgggc ctcgcccgtc acgccgtcga gctcgcgcag gacccggggg atccagtcgg
     1861 actcctcggt ccagcgcgcc gagtggatgc cggggcggcg gtccagcgcg tcgatctcga
     1921 tgccgctgtc gtcggcgatg gcgagatcgc cggttgtctc gcagacggcg cgcgccttga
     1981 gcagcgcgtt ctcctcgaag gtggtcccgg tctcctcgac gtctgggtcc tgggccgaga
     2041 cggcccagcc ggggccgagg atgtcggcga tctcgtccac cttgtgggcg ttgcccgtgg
     2101 ccagcaccac cgggcgcggc ccgccgctca tcgctggacg ggccggggag ccggcgcctc
     2161 ggccacgtag gcccgctggc gctcgacgag ctggccgatg ccctgctcgg cgagatcgag
     2221 cagctggtcc agcacgggac gaccgaaggc cgcgccctcg gcggtgccct gcacctcgac
     2281 gaagctgccc tccgacgtca tcaccacgtt catgtcgaca tcggcggtcg agtcctccgg
     2341 gtacggcagg tcgcacacgg gcacgccctt caccacgccc acgctgatgg cggcgcagaa
     2401 cgcggtgagc gggtgctcgg cgatgtcgcc ggccgccacc cgtcgggcca gcgcgtcgtg
     2461 gagtgccagg tagccgccgg tgatggcggc ggtgcgggtg ccgccgtcgg cctggagcac
     2521 gtcgcagtcg acggtgacct ggcgctcacc gagcaggcgc atgtcgcaga cggagcgcag
     2581 cgaccgcccg atgaggcgct ggatctcctg ggtgcgcccg ctctgcttgc ccttggccgc
     2641 ctcacggccc acgcgctcgg ggctcgagcc ggggagcatc gagtactcgg ccgtcaccca
     2701 gccgctcccc cgccccttca tccaccgggg cacgtcgtcc tcgaccgacg cggtgcacag
     2761 caccttggtc tgcccgaagg agatcaggca ggagccctcg gccatgaccg tggggtcgcg
     2821 ctcgatcgtg accgggcgca gctcgcccgg ggcgcgcccg tcgccgcgtg tcgtctcggt
     2881 gtcggtgctc atgtgtgcgt cctcaggctc ggtggatggc gtcgatctcg gccacctcga
     2941 tcgggccgtc gtagacccgt gccgcgtcgc tccgacgggc ctcgggatcg ctaccgggag
     3001 cgaggtgggt gaggaccaac gttgccacat cggcctcggc ggcctggcgg ccggcctggg
     3061 acgcggtgag gtgctggacg acatcggcgt cggcctcgtc gagggtggcc tcgacgatgg
     3121 ccagatcgat cgagcgcccg atgtccggac ccagcgacgc caaccgccac gcatccccgg
     3181 tgtcggccga gtagacgatg gagcgccccc cgtgctcggc ccggatcgcc agggtctcga
     3241 ccgggtggtc ggtgcgtgag aaggacagct cgatgtcgcc gatggtcgcc gtgctgcgct
     3301 cgtcgatgac ctggggagcg aaggtcggcg ccggctcggc ggcgatggcg gcgaactgct
     3361 cggccgtgcg cgccgtccac agcagcggca tcccgagatc cggcgcaccg acgccgtacc
     3421 gcagcgcgtt gagcagcacc gggagctcca gccagtggtc cgggtgcgag tggctgacca
     3481 cgatgccgtc gagctcgagg agggggacgt gccgctggat gttgcccagc gacccggcgc
     3541 cgcagtccat ccacaggttc gtcgtctcgg tgcggagcag gtagccgctg cacgcaccgc
     3601 ccgcccccgc gtaggtgccc gagcacccga ggatggtcac cgacaggccg ggcggggcgt
     3661 tcacctctcc caccgtacgg cgctggccac gtccagctcc ggcccgagca gccgggcacc
     3721 gagggcgcgg aagacctcga cgtcgcccga gctgaaccac tgctggcggc cgcgtccccc
     3781 gcggtcggcc ggcgcccacg aggactcgag catcgcccgg acccggaacg ccgtctcgtc
     3841 ggcggaggac acgagcacca cgtcgcggcc gaggacgtcg gacagcgtgc gggccaggac
     3901 ggggtagtgg gtgcagccga ggagcagggc gtcgactccc gcctccacga ccggggcgag
     3961 cagccgttca gcgagcacgt gcacctggtc cgagtcggtc tccccccgct cgacgaactc
     4021 gacgaagccg gggcaggcgg cgcaggtgag ctcgaggtgc ggggcggcgg cggcgacctc
     4081 tcgctggtag gcccccgagg agatggtgcc gaccgtgccg atgacgccca cccgctggtt
     4141 gcgcgtggcg agcacgagcg acgcgacgcc cggctcgatg accccgacca ccggcacggg
     4201 cagcgctgcc tgcaactcgt ccagcgcggc ggcggtggcc gtgttgcacg ccaccacgac
     4261 caccttcacg tcgtggacct ccaccagcca gcggctgatc tggtgggcga agccacggac
     4321 ctcctcgagc ggtcgcggcc cgtaggggta ccggcccgtg tcgccgacgt agaccaggtc
     4381 ctcgccgggc gcgaggtcga tcagggcccg ggcgacggtg agcccgccga agcccgagtc
     4441 gaacatgccg atcggccggt cgtcccccat cgctcaggtc tggtcgccgg gctcgtcggc
     4501 gaccatcgtg accacccgct gcgggaaggg gatctcgatg ccctcgtcgt cgaaggcgtc
     4561 cttgatggcc atggccaccc cggagcggac ccggaagacc gtggcgatgt cggacgcggt
     4621 ccagaaccgg acgtcgaagt cgatgctgga ctcgccgaac gtcgtcaccc aggcctgcgg
     4681 cgccggcgcg gggatgacgc cgtcgacgcc ggtgacggcc gcgagcagga cgtcctgggc
     4741 gcggtgcaga tcggtgccgt aggcgacgcc aacgggcagc acggtgcggc gggtcggcgt
     4801 tgccgtgtgg ttgacgatca cgtcgtccat gaccatcgag gacgggatgt acacccgctc
     4861 gccgtcgaag ctgtcgagca cgacggcacg gaagttgatc tcggtgaccg tgccggcgcg
     4921 gtcgttggtc cagacctggt cgccgacgcg gatcgggcgc ctggtcttga gcatcaccga
     4981 cgcgaagacg ttctcgagga tggcggtgac ggcgaaggcg atggcgatgc cggtgacgcc
     5041 gagggcaccc accagcggtc cgatccgcac gcccagcgcc gtgagggcgt agagcacccc
     5101 gaccagcacc accaggttgc gcacgagccg ggcggagaag cgggcgatgg ccccctcggg
     5161 atccgtgcgc cgcacgaggc gctcgacgac gcgacgcagg accatcgcgg ccacgacgaa
     5221 gccgacgaac gtggccccgg ccaccagcca gtccgtggcg gtcaggccct cgcggaccac
     5281 ggcgtcgagc tcgacctcgc ccgtcggcac ctggcggagc aggctcgaca ccgcagggcc
     5341 ggtgacggtg gtggcggggt cggcgaccat ggttgacagg tgctcctgca agcggttaca
     5401 tcgaagtccg aacgggctgc gaggatggca cagcgtgggg gatcaggtga ccgatgccgg
     5461 atggtggtcc gatgcagtcg gctacgaggt gtacctgcgg tccttcgccg actccgacgg
     5521 tgacgggatc ggtgacctcc ccgggctgac ctcccgcctc gaccacctcg ccgacctcgg
     5581 catcgacatc gtctgggtca cgcccttcta cccctccccc atggccgact tcggctacga
     5641 cgtcgccgac cacaccggcg tcgatccccg cttcggcacg atcgaggacc tcgaccgcct
     5701 cgtcgccgcc gtgcacgagc gcggcatgcg cctgctcatc gacctggtcc ccaaccacac
     5761 ctccgaccag cacgagtggt tccgctcggc cctgaccgga cccgggagcc cgcaccggga
     5821 cttctacatc tggcgcgacc ccgcccccga cggcggtcca cccaacaact ggcgcagcca
     5881 cttcggcggt ccggcctgga ccttcgacca ggcgtccggg cagtggttct gccacctctt
     5941 cctccccgag cagcccgacc tgaactgggc caaccccgag gtgcgggccg cgttccgcga
     6001 catcatcacc cactggctgg accgcggggt cgacggcttc cgcatcgacg tcgcccactc
     6061 gctcctcaag cacccgtcgt tcgccgacaa cccggtcctg gccgaggtcg gcgcagatgc
     6121 ctcggcgggc gaggcgttcc aggccatgcg ccacgaccac gacctcgacc aacccgagac
     6181 gctcgacatc taccgcgagt ggcaccggct ggcccgggcg cacgacgccg tcctgctcgg
     6241 cgaggtctac ctcgactctg ccgaagcggt cggcgcctac gtggccgacg gggtgctcga
     6301 cctgtcgttc ttcttccccg tgctgcacac gacgtgggac cccgacgtgc tccgcgacgt
     6361 ggtgaccgcg accctcaccc acgggcgggg acggttcgcg tgggtgcact ccagccacga
     6421 cgatcgccgc gcccccaccc gcttcggtgg cggcgagcag ggacggcgcc gggcgctctc
     6481 cttcctggcc ttcaccgcgt tcctgcccgg accgctgttc ctctaccagg gcgacgagct
     6541 cgggctggtc gacggcgagg tcgacccgga ctcggcagcg gatcccgtgg ccgtgcgcaa
     6601 caccaacccc gacggcgggc gcgacccggt ccgcacgccg atgccgtggt cgcccgatgc
     6661 acccaacctc gggttctcga ccggcacgcc gtggctcccc atgcccgcca accacacggc
     6721 agccgacacg gtcgaagccc agcgcgctga tcccggatcg acgctgcacg ccgtgcgccg
     6781 gttgctgcag ctgcgagacg agcgactgcc gaacccggag cccgtgacgc tgagcgcgga
     6841 gggtcccgcc gaggtgctcg gctaccgccg cggcgacgtg ctcgtcaccc tcaacgtcgg
     6901 gtcccgcgcg gtgaccgtgc cactccaggg cccggtcgag ctggcgttcg gcaacggcgg
     6961 cgtggacctc ggtggcgacg cggtggtgct cgagcccgat gccgtcgccg tgctggacgt
     7021 gctccggtga gccccaccga caccccgcga cgccggaccc ggacccgcac ccgcaccgat
     7081 ggcgtcacca tcgaggaggt cgcccggcgc gccaacgtgt cggtggcaac cgtcagccgg
     7141 gcgctgcggg ggctccccaa cgtcgccccg tccacgcgct cgcgcgtcga gcaggtggcg
     7201 cgggacctcg actaccaggt cgacacctac gcctcgcgcc tggccaccgg gcggagcgac
     7261 acggtcggcc tcgccgtgcc gaacatcaac agctggtact tcgcccaggt ggcggccggc
     7321 gtcgaggccg tgctcgccga cgccggcatg gacgtgctcc tccagtccgt cgacgacgga
     7381 gccggtcgca cccggctgct gtcggcgcag tcgtccgtcc gacgccgcct cgacgggctg
     7441 atcctgatcg acgtcctgct cacccccggg gagatccgca cgctcgccgc cgactcggtc
     7501 cacgtcgtca cggtcggcca gcacaccgac gagttcccct ccgtcaccgt cgacaaccgt
     7561 cgcgccgcac tggccgccac ccgccacctg ctcgagctcg ggcaccggcg catcggcctg
     7621 atcggcgggt cgacgcacac cggcctgccc ttcgacgtgc cggggcaccg gcggcagggc
     7681 tacgtcgagg ccctgctcga ggctgggctg gactaccacg ccgacctcga ggccaacggc
     7741 ggcttctcgg tgaagggcgg gtccgaggcg atggtccacc tgatgagcga ggcccgtccg
     7801 ccgagcgccg tgttcgcgct gtccgacgag atggcgatgg gtgcgatcaa ggcggccagg
     7861 gacctcgggc tccgggttcc cgaggacgtg tccgtcgtcg gcttcgacga ccacgacctg
     7921 gccaaggtgt tcgagctgac caccgtccgc caagaccccg agtggcacgg cgccaccgcc
     7981 gggcggctgc tgctcggcct cggcgacgag cccgctcccg aggtggccca cgtcgtgggc
     8041 gagacccggc tgctggtccg ctccaccacg tcgtcaccgg gtggtcattg acacggcaca
     8101 ggtgccgttg taatcgttta catgccttcg gggcctgggg aaccgggtcg cggaggactc
     8161 agccagcaac acaggggaag acaaatgaaa cgaccactgt ggcggatcct cgccgctctc
     8221 ttcgcgctct ccctcatcgc ggcgtcctgc gccggtgacg acgacgacac gagtgccgga
     8281 gacgattcgg gagatgactc cggcgattcc ggtgactccg gggacggcgg cgacgtcgag
     8341 atcgagagcg tcgacgtctt ccccctcccg cccgtcggtg actccggtga ggtcgtcctg
     8401 accggcggca ccttcgccgg tgcgttcagc gccgatgagg acaccatggc ggtcctcgag
     8461 tacatggcgt ctcccgagta cgtggagcgc cgcctcgagg ccgaggtcgg cggcttcctg
     8521 tccgccaacc agaacgtcga cctcgagtcc tacagcggct tcgagcagac gctcgtggag
     8581 atcctccaga acgccgaggc cgcccgcttc gacggttccg acgacatggt cgccgaggtc
     8641 ggcgccggca ccttctggtc tgccgccacc gacatcgtct cgggcaacgc cagcgtcgag
     8701 gaagccttcg ccgaggtcga gtccggctac ccgccggtcc ccgagatcgg cgagtgcccg
     8761 gcccccgaga tcggccccgg tgacggcacc gtcaccatct gcggtcccga ggtcgagcag
     8821 gagcagcagg gcttccagca gtcgttcgac gccttcaccg aggagaccgg catcacggtc
     8881 gaggtgcagg gcgaccgcag cgccgaggag cagatcggcg tgcaggtcca gggcggcaac
     8941 ccgccggacg tgttcatctt cccgcagccg ggcctcctcc gctccttcgt ggaggactgc
     9001 cagctgacgc cgctgcccga gaacgtcgtc tccgccatgc aggagaactt cgtcgagggc
     9061 ttcatcgccg gtggccaggt cggcgaccag tactacggcg tgccgaacaa gtcggacgtg
     9121 aagtccctcg tctggtactc ccccgaggcc ttcgaggcca acgactacga ggtccccgct
     9181 gaccacgacg agttcgtggc cctgatggac cagatggtcg aggacgggaa caccccgctg
     9241 tgcatcggca tcggttccga tgccgccacc ggctggccgt tcacggactg gatcgaggac
     9301 tacatgctcc ggctgcacgg ccccgaggtg tacgaccagt gggtcaacca cgagatcccc
     9361 ttcgacgacc cccaggtcgt cgaggtcggt gagttcgtga tggacatctg ggagtcggag
     9421 ggctacgtct tcggtggcat gcaggccgcc gcctcgactc ccttcgccga gtccggcctc
     9481 gggatcatcg agggcgactg catgatgcac cgccaggcca acttctacgc cgccaactgg
     9541 cccgacggga tccaggtggg ctcggagtcc tgagccagca ctgagcgatg ccgatgccgt
     9601 ggttccaccg cctcccagag ccggtgggac cgcggcatcg ccgcgtccgg gcgacgtacc
     9661 atcagcgctc ggacgccagc cgaccgacgt ccagggggga caccacatga ccgcacccac
     9721 caccgccccg acgaccgggg gtgaggaccc cgacggcaca ccgccggacg gcgcgggcac
     9781 acctcgcccg cgaggggcca ccgggtccaa ggccgacgcc gcccagcagc tggtggtggc
     9841 gggcgtgctg ctcgccatcg ccgtcgcctt ctggttcatc gactcgctgt cggactcggc
     9901 gctgatgatc gccctcaccg gcgtggtgat ctccatcgcc gcctcggccg ggatcttcat
     9961 cggcgccaac ggactcgcca gcctcctgcc gggcaagacg gccgagaagc tccgcccctg
    10021 gatcttcgtc gggccggcgc tgctgttcat cttcgtgacg ctggtcgtgc ccaccgtgcg
    10081 cacgctctac ctgtcgctgc tcgacaacgc gggcaacgag ctcgtcggcc tcgagcagta
    10141 cggcgacatc ttcacctccg acgacatctt cggcttcgac ggcatcgccg acatgttcac
    10201 cagccgtctg ttcatcctcg ggctggtggc gctcgccgtg gggatcgtcc tcgccgtccg
    10261 caacggacgt gcgatcggca cccgcaccga cttctcgtca tccggcacca ccctggccat
    10321 cgccgccggc gtcaccctgg tcctgttctc gctgttcacc tccgcccgag gcgtcatctg
    10381 gaacaacctc tggtgggtct tcgccgtgac cgcgctggcc accgccctcg gcctggccgt
    10441 ggccgtgctg gccgaccggg tcaagggcga ggcgatcgcc aaggcgctga tcttcctgcc
    10501 gatggcgatc agcttcgtcg gcgcatcgat catctggcgc ttcgtctacg cgttccgccc
    10561 gcaggcctcg gaccagatcg gcgtgctgaa cgccgtgtgg cgaggcctcg gcggtgcggt
    10621 cctcgacccg ctccaggccc gaccctggaa cacgttcttc ctcatcatcg tgctcatctg
    10681 gggccagacc ggcttcgcca tggtcgtgct ctcctcggcc atcaaggcgg tgcccgacga
    10741 gtacatcgag gcggctcgcg tcgacggggc caccgagtcc cagatcttct ggcggatcat
    10801 cctcccccag atccgctcca ccatcctcgt ggtcgtcacc accttgatca tcctcgtgat
    10861 gaaggtgttc gacatcgtca aggtcatgac caccggcgag ttcggcacga acgtggtcgc
    10921 caacgagatg ttcgaccagg cgttccgctt cggcaacgtc ggcctcggtg cggcactggc
    10981 atccgtgctg ttcctcgccg tcctgccgct catgtacatc aacgtccgcc gggtgcgcga
    11041 ggagtcacag ctgtgagtgc gatccagagc gagccggggc gccgcctcga gcccgagggg
    11101 gaggaccccc gcgaccacgt cgtcgagagc gagctcccac cgcgggggcc gcggtctcgc
    11161 acgaccacca tcgtcgccat cgtctccatc gtcggcggcg ccatcgcctt cgtgtggggg
    11221 atggactggt tcctgttcgg cctctccgac gagatcgcca ccctgaccgt gggtggcctt
    11281 gcggtgatgg tgctcggcgc ctggtccgcc atcggcggag cgctgctgct caagcgggtg
    11341 tcgtgggcgc agccggcgat ggtcgcgacg tacggcgtcc acgccctggc catcaccgtg
    11401 ttcggcctga tctccgccgc cgccagcggt ggcggcctgg tcgactacct catccccggc
    11461 gtgctgctcg ctgccgcgct ggccatggtg tggatgctgc gctcacccgc cgtcgagaag
    11521 gacttcccgt ccgacagcgg gtccggcctc ggtcgcacgg tggcgctgtg gtcgatcgtc
    11581 gccgtgtggt cgctgcccac catcggcctg ctgctctcct ccttccgcgc cgagcgggcc
    11641 gtgaagacgg agggctggtg gacgatcttc accaacccgc agctgaccat ggacaactac
    11701 cagacggtcc tggagtccgg cggcagctcg gcacccaaca tgttcgaggc gttcctgaac
    11761 tcggtggcca tcgtcctacc ggccacggtg atcccgatct cgatcgcgct gttcgccgcc
    11821 tacgccttcg cgtggatgga cttccccggc cgcaagtggc tcttcgtcgc cgtcgtgtcg
    11881 ctcatggcgg tgccgctgca gatgtcgctg atcccgctgc tgcagctgta cgtcggcggc
    11941 gcccacatcg gcagcttcac gatcttcccg gacctcgatc tcaacggaaa ggccgtgtcg
    12001 gtgtggctga cccacaccgg gttcggcctc ccgctggcca tcttcttgct ctacaactac
    12061 gtgtccgggt tgccgaagga catcttcgag gccgcccgca tcgacggcgc cgaccactac
    12121 acgatcttct ggcgcctgat cgtgccgctg tccgtgccgg cgctcgccgc cttcggcatc
    12181 ttccagttcc tgtggacctg gaacgactac ctggtggcgc tcaccttcct cggcggcaac
    12241 gccgacaccc acccgctcac gatgcgcctc gcgaacctgt cgggcggacg ggggcaggac
    12301 tggcacgtgc tcaccgcggc agcgttcgtg acgatgagcg tgcccctcgt cgtgttcttc
    12361 agcctgcagc gctacttcgt ccgaggcctg ctggccggat ccgtgaaggg ctgacgcccg
    12421 atcccagacg cgagacgggc ccgggcatct gcccgggccc gtcgtcgttc tcgtgtgccg
    12481 ttcagcgcag cgtcaggccg gtcttggagt cgaagaagta gaggttccgg gagtcgatgg
    12541 ccacctcgat ggtctcgccc cggttcacac ggctgcgggg gctgaaccga ccgaccaggg
    12601 gtgtgccggc ggcaccggcc gtgccctcgt cgttcacgtc ggggtcaccc gagtggacct
    12661 tgggcgcatc gatcgaggcg tggaccatca gctcggagcc gagcgcctcg accagctcga
    12721 cgtcggtctt gatgagctgg tcgcgagggt ggtcgggctt gagggaggcg tcctccatgt
    12781 cctcggggcg gatgcccacg gtgaccttct cccccaggtg gtccttcatg cccggcttgc
    12841 gctcgagcac gtcggccggc acggagacgg tctggctccc gagcttcagc gaggcgccac
    12901 cgtcggaccg gctcagcacg ccctcgtaga ggttcatgga cggcgacccg atgaaggcgg
    12961 cgacgaagac gttgtcgggg tggtcgtaga ggttctgggg cgtgtcgacc tgctggagga
    13021 tgccgccctt caggaccgcc acccggtcgc ccatggtcat ggcctcgacc tggtcgtggg
    13081 tgacgtagag ggtggtgacc ccgagctcgc gctggatgcg ggcgatctcg gcccgcatct
    13141 gcacgcggag cttggcgtcg aggttcgaca gcggctcgtc catgaggaac gccgccggct
    13201 ggcgcacgat ggcgcggccc atggccaccc gctggcgctg gccgccggac aggttgcccg
    13261 gcttgcggtc gaggttctcg gtgagctcga ggatctcggc ggccttgtgc acccgcttct
    13321 cgatctcgga cttggagacc ttggccagct tcaaggcgaa ggcgatgttg tcgaacaccg
    13381 tcatgtgcgg gtacagcgcg tagttctgga acaccatggc gatgtcccgg tccttcggtg
    13441 cggcctcgtt cacccggcgg cccgcgatgc gcagctcccc cctggtgatg tcctcgaggc
    13501 cggccaccat ccgcagggcg gtggacttcc cgcagccgga tggaccgacc agcaccagga
    13561 actctccgtc ttggatgtcg acgtcgaggt ccttcacggc ctcgagtccg tccggataca
    13621 ccttcgagac cttgtcgagg gtcacttgcg ccacgacgtt ccccctgtcg ctcggctggc
    13681 tgaaagcgct tgcagcgtac accgcacccc ggcggcgtgg ggccagaact cgatgatctt
    13741 cgatcagaag tagatgatct tctcggcgcg ctccacgacc tcgtcgaggt cgtcggtcca
    13801 cgcaccggtg gagatgtact tccagccgta gtcgcacacc acgaacacga tggtgccgga
    13861 ctcgatctcg ctcgccacct tcaccgcgcc ggccagggcc gcgccggcgg agatgccgga
    13921 gaagatgccg atctcagaga ggcggcgcgt ccactcgatg gactcgcggg ggcgcacgat
    13981 gcgcttgcgg tcgagcagct ccgcgccgcc ccacttctcg aagacgggcg ggatgtagcc
    14041 ctcgtccagg ttgcggaggc cctcgacgcg ctcccccagc gggggttcga tggcgatcac
    14101 ctggatgtcg gggtcctgct ccttgaggaa ccgtcccacg cccatgagcg tgccgctggt
    14161 gccgaggccg gcgacgaagt gggtgatctc ggggacgtcg gcccagatct cggggccggt
    14221 ggtgcggtag tgcgacagcg ggttggcctc gttcgcgtac tggtacagga acgcccactc
    14281 ggggtgctcg tcggccagct tgcgggcccg gaccaccgcg ccgttggagc cctcggcccc
    14341 cgggctcatg atgatctcgg cgccgtagat ctccagcagc tggcggcgct cgatcgagac
    14401 gttctcgggc agcacgatct tgatcgggta gcccttcatc cgggcgatca tcgccagcgc
    14461 gatgccggtg ttgcccgacg acggctcgat gatggtcttg cccggcgtca gggtgccgtc
    14521 ggcctcggcc tcctcgatca tggccagcgc gatgcggtcc ttcaccgagc ccgccgggtt
    14581 ctggccctcc atcttgccca ggatccgcac gtcggggttc gggctgagcg cgctgcagtc
    14641 gacgatgggg gtgttcccga tcatgtcgag gaccgagtcg aagactgcca tggtcgcctg
    14701 ggctcctggt gtcggcgtgg aaagttgacc gcgtgggtca acaatgtacc cgccggtcgg
    14761 tgttcccgat cagcggcaca tcctcagcgg acgatgccga cgggctcctc ggtgacggtg
    14821 ccgtcgacga tccggtagct gcgcaggacc ggcgcctcgt cgcggaggga cacgatcacg
    14881 tagtgccagt ccggatccgg tgcctgctcg atgtcggtgg gcgacgggta ggcgtcggtg
    14941 tgggtgtggc tgtgcacgac gccgatgatg gcgaggccgg cctcggccgc ggcgtcgtcg
    15001 gcgtcgaggt agtcccgcgg cccgatgctg tacacccgcg acgaccgggc cgcgttcgcc
    15061 gtggggtgga agatcggggc cgaatcccgg gcgggatcgc cgaggagcag cccgcaggcc
    15121 tcgtcgggca agccgtcgta ggcgtgaccg acgatggcgg cgtaggcgga ggcggcgagt
    15181 cgcagcacgg cgggcgaacc tatccgccct caccctcggc gagcgtggtc tcgaccggga
    15241 ccgaggtggt cggtagcgga gggggcggcg ccgacggcga ctgcccgggt tcggtgcccg
    15301 tgacctccca gtgccacagg tactgccgca gcgtcaggcc cgacgcctgg acccgcccgg
    15361 cgaggtcccg tcccaggtag cggaagtgcc agggctcgta cttgtagcag gtgacgtcct
    15421 cggcaccggc cgggtacgac agcacgaagc cgtgctccca ggcgtgctcg gccagccact
    15481 ggcccgtggg cgtctccccg aaggcctggt ccacgtcggt ctggccgatc gggcgcacgt
    15541 cgatcgtggt gccgaggtgg tgctcgctgt gaccgccccg tgcggtgccc tcggctgcgg
    15601 ccccggcgcc gagctcggcg accctggcgt cgaagagccg ctgctggtcg gcgatcgagc
    15661 ggtacccggc gaggagggcc acctccggga ggccttcggc gatcagggcg ttgcgcaggc
    15721 tggcgaggtc gtcgacgacc agggcgcgga tgaggtactc ggcgctgtac cccgctgccg
    15781 acgccgggac caggtccggc ggctcgtact gctcggggac ggcccgcatc gggtcgacca
    15841 gggtccggta ccagtcgtcg gtgcggtcgt aggccgtggg ctcggactgc tcggggttgg
    15901 tgcagggcgg cggcggctgc agaggcaccg tcgtcgtggt cggctgcgtc gtcggggcca
    15961 gggcggtctc cgagtcgtcg tccccggtga ctgcgcccac gatgttccag accaggagca
    16021 cgacgagcag cacccccgtg gcggccacga ggcgacgcag ggcgtagttc ggtcgaggtc
    16081 ccggtcggcg cggcgggtag ggctgcatcg cgccgacacg ctaggcaacg ggatcgagcc
    16141 ggaccgggag ggtgccggta gcgtcgcgga cgccatgccc gccgccgaca ccaagctcat
    16201 cgccgcgaac cgccgcgctc gacgcaacta cgacatcacc gagaccctgg aggccggcat
    16261 cgtcctgcac gggtccgagg tgaagtcgct gcgcgaggcc acggtgcaga tcgccgacgg
    16321 cttcgtgcgc ttcgagggca aggaggcctg gctgcagggc gtgcacatcg ccccctacct
    16381 gcactcccag gaccacagcg ggcacgaccc cgaccggcac cgcaagctcc tcctgcaccg
    16441 gcgcgagctc gaccgcctcc gcacgatggt cgacctcgag cgggtgtcga tcgtgccgct
    16501 gtcgctctac ttcaaggacg gccgggccaa ggtcgagctc ggtgtcggca agggccgcaa
    16561 gctgcacgac aagcgcgagg tcgaggccaa gcgcgacgcc gatcgggaga tccagcgggc
    16621 actcgcccag cgcagccgct gacccactgg tgacatacct cctggggtac caggtgaccc
    16681 tggaccctgg cttgccgccg gaggcggtcg cacactccgg acatgaccaa gaacgtcgtg
    16741 atcctcggtg ggggcaccgg cgggacgctg accgccaacc gcctgcgcaa gcagttcaag
    16801 aagagcgagc tcgccctcac ggtggtcgac caggaccgcg accacgtgta ccagccgggc
    16861 ctgctcttcc tgcccttcgg gctggcccac ggcgaggacc tggtccggcc ccgcgatcga
    16921 cagctcaaga ggggcatcga cttcgtcgag tccggcatcg accgagtgga cgtcgagggc
    16981 cggcgggtcg tcctgtccgg gggcggcgag ctcccctacg acgtgctggt gatcgcgacc
    17041 ggcgcccgcc tcgtccccga agagaccgac ggcctcaccg gaccggggtg gatggagaag
    17101 gtcttcacct tctacgacct gccgggggcg accgggctcg agcaggccct cgccgacttc
    17161 gaggggggcc gggtcgtggt gaacgtcgtc gacatgccca tcaagtgccc ggtggcaccc
    17221 ctcgagttct gcttcctggc cgattggtac ttccacgagc gcgggatgcg cgatcgcgtg
    17281 cagctcaccc tcgtgacgcc gctcgacgcg gcgttcacca agccccgggc ggcgtcgatg
    17341 ctcggcggca tgctcgagga gaagggcatc gagctggtca ccgagttcaa cacgggcgag
    17401 gtcgacgggg tggcggggag gctcgtcggc ttcgacgagc gggaggtccc cttcgatctc
    17461 gccgtggtgg tcccggtgca ctcgggcgcc gagtacgtgg gccgctcccc cggcctcggc
    17521 gacgccctcg acttcgtcga gaccgatccg cacacgctcc agtcgaaggc ggcgccggag
    17581 atcttcgcga tcggcgacgc cgccaacctg ccggcgtcca aggccggctc ggtgagccac
    17641 ttcgagggcg aggtgctggt cgagaacgtc acccgcttcc tccgcgggga accgctcgac
    17701 gccagcttcg acgggcacgc caactgcttc atcgagaccg gcttcgacaa ggccctgctc
    17761 atcgacttca actacgagca ggagccggtg gagggtcact tccccggccc ggtgggcctg
    17821 cccctgctga aggagtcgcg gctcaaccac ctcgggaagc tgatgttcca gtggttctac
    17881 tggcacagcc tcctgcccgg gcgggacatc cccggcgtca aggccgacat gccgaccgcc
    17941 ggcaagcaca tcgactgagc accaaggagc gcaacgacaa tgagcaccgc aaccatcgcc
    18001 ggcagccagg tcgagctgaa cgaagagggc ttcttcgtcg atcccgacca gtggaccgag
    18061 gagatggtcc ccgagctggc cgccaaggaa gacatcgacg agctggacga caagcactgg
    18121 caggtcatcc ggttcatgcg ccaggagtac ttcgcccacg gcaccggccc caccgtgcgg
    18181 gccctgggga agacctccgg ggtgtcggtc aaggagctgt accagctgtt cccgaagggg
    18241 ccggcgaaga tcgccgcccg catcgccggg atccccaagc ccaagggctg catctgacga
    18301 cgtcagacca acaggaggac ggaacgacca tggacctgag cacgcacaag gtctcgatca
    18361 tcgtgtcgca gggatcgctc gagggcatct accccgcgct gatcatggcg aacggcgccc
    18421 gagccgaggg gatggaggcg aacctgttct tcaccttctt cggcctcgat gccatccacc
    18481 gcaagcgcca cgagcacatc aagatgtcca cggtcgggaa cccggggctg cacatggcga
    18541 cctgggccgg cggcctgccc ggtgtgtcgt cggtgatgac ccactacatg gagcacaaga
    18601 tggaggcgct cgacatccct tcgatccccg agttcgtcga gatgatctcc gacaccggcg
    18661 ccggcttgta cgcctgccag gcctcggtcg acctgttcgg cctcaccaag gacgacctca
    18721 tcgaccaggt cgaggggatc atcaccgtgg gcgacttcta cgagatggcg gtcgacggcc
    18781 acatcatcta cacgtgaccg gcccccatcg ccgccggcag taggttctcg gtcgtgatgc
    18841 gaccgtgagc ggagtcacgt tcgagcaggt gaccaagcgc ttcggtgatg tcgtggcagt
    18901 ggactcggtg tccctcgaga tccgggacca ggagttcatg gtcctgctcg gaccgtcggg
    18961 ctgcggcaag tccaccgcgc tgcggatgat cgccgggctc gagacggtgt ccgcaggtga
    19021 gctgcgcatc ggcgacacga cggtcaacga cgtcgaggcc aaggaccgcg acatcgccat
    19081 ggtgttccag agctacgcgc tctatccgca catgaccgtg cgggagaaca tcgaggtgcc
    19141 gctgaagacc cgctcgtacg acgtcgacgg cgacaagcgc aagctgcgca agcccgagcg
    19201 ggctgaccgg gtcgccgagg ccgcccgcat cctcggcctc agcgagctgc tcgaccgcaa
    19261 gccgggcgcg ctctcgggcg gccagcgcca gcgggtggcc ctggcccgcg ccatcgtgcg
    19321 ccggcccgag gtgttcctca tggacgagcc gctctccaac ctcgacgcca agctgcgggc
    19381 ccagacgcgg gtggagctcg tcgagctgca ccgccagctc ggcaccacga tcgtctacgt
    19441 gacccacgac caggtcgagg ccatgaccat ggccgaccgc atcgcggtga tggaggccgg
    19501 gaagctgcag caggtgggcg cgccgcgcga cgtgtacgcc cggcccgcca acctcttcgt
    19561 ggcccgcttc atcggcagcc cgccgatgaa caccgtcgac ggccgcgtcc gcgtcgacgg
    19621 cgaccgagca ctggtcgagc tcggcgacgg gcacctcgac atcgggcctc gcgccggggt
    19681 cgccgacggc gacgccgtgg tggtgggcat ccgccccgag cacctccgca tcgccgagga
    19741 cggcgtgtcc gcgcacgtgc gcgccatcga gtggctcggc cacgagcgtc acgtgtactg
    19801 cgacctggcc ggcacgccgg tgatcgtgcg cgacaccgac ccgggcgagg accacgccga
    19861 gcccggggcc gaggtgatgg tgcaggccga cccgggcacc gtccacctct tcgaccccac
    19921 caccaccgag cggatcgact gatgcccgtc accgagcggt cccgcaccga gccgccgtgg
    19981 tggcggcgcc ggcggacccg cgaggccggc ctggcggcgc tcttcctcgc gccgtcgatc
    20041 gtggtgttcg gggcgttctt cttctatccc atcgggcgca tcgtctacct ggggttcttc
    20101 cagcagaacc agacacggac ccgtcagcgt tgggcgggca ccgagcagta cgtggaggtg
    20161 ctcaccgggg aggagttccg ggagggcctc ggcatcaccg tccagtacgt gctctacacc
    20221 gtgcccgccg ggctggtgct gggcgtgttg ctggcggtcg cggccaaccg ccgcctccgg
    20281 ggcatcaaga tcttccacac gatcttcgcg tccacggtgg cgacgtccgt cgccgtggcg
    20341 tcgatcgtct ggctggtcct catcaacccg accgtcggct acctcgaggt cgactggctc
    20401 cgcgaccccg accgagcgct gttcggggtg gcgctcagct ccatctggca gaacctcggt
    20461 ctcacgttca tcatcgtgct ggccgggctg caggccatcc ccgacgagat caacgaggcg
    20521 gcgacggtcg acggcatgag cgcgcccagg cggttcttca ccgtcacgct cccgatgctc
    20581 tcgccgaccc tgctcttcct cgtggtcgtg ctgaccgtct tcgccttcca ggccttcgcc
    20641 cagatcgaca tcctcacgag cggtggtccg gccggctcca ccgagaccct cgtgttcaag
    20701 atcttccagc agcagcaggg gggttccgtc ggcgagggat cggtgatggc cgtggggctc
    20761 ttcgtcatca ccttcctcg
//
LOCUS       DATISU010000247         4822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_13767, whole genome shotgun sequence.
ACCESSION   DATISU010000247 DATISU010000000
VERSION     DATISU010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13767"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VLR27_09925"
     CDS             <1..241
                     /locus_tag="VLR27_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PPOX class F420-dependent enzyme"
                     /protein_id="HSP03808.1"
                     /translation="GRVSMVVDDEHPPYSFVKVDGTVTLSEDLDEVRRFATIIGGRYM
                     GADLAEEFGARNGVPGELLVRVHPTRVLAAYDIAD"
     gene            450..1031
                     /locus_tag="VLR27_09930"
     CDS             450..1031
                     /locus_tag="VLR27_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HSP03809.1"
                     /translation="MNKIRIAGAVAGTALVSGMVLQVSSAAFSDTTENPGNSWAAGTV
                     ALSDSRTGSAMFAATNMVPGDSESQCIDVTYDGSVTPSEAIALHAEVTESADAVGNGL
                     GDDLDVTVEMGPADTDCTTSAAGAGTSIYTGTLAGFHAAADPLATGWTPDAADGAADI
                     SRPFWFTITLGDDTPNDAQGDGATAAFTWSATS"
     gene            1065..2096
                     /locus_tag="VLR27_09935"
     CDS             1065..2096
                     /locus_tag="VLR27_09935"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HSP03810.1"
                     /translation="MTATTLPTPAGTAAGAGWLPSLAGQVLEFVWPMFLTLVLTLGAW
                     VLGPTLLLGWQPVAIISGSMAPAVPPGHVVLVEPFRGQDVGPGTVVTYRDPRQDRLVT
                     HRISAVAEDGTITTRGDSSVVDDPVPLSTDRIVGVGRLVVPAAGLPALWAHDGRVDLL
                     AGVAVLTVLTFGSAASAAATGVRSLRSRRLSLRRGARPPASPTVGLVTGVASVLAVLA
                     TSAVSVAAFVGEGQNPANTFRAGTVPAPTITAATDACPAADPNARGGKRAIELAWDAI
                     PNASAYEVERATDPAGPFTPLGTIPDTCMRDTDVNAATTYHYRVRAHAGPWTSAWGTT
                     SAELPRGPR"
     gene            2311..3966
                     /locus_tag="VLR27_09940"
     CDS             2311..3966
                     /locus_tag="VLR27_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HSP03811.1"
                     /translation="MDGLMQDAPLTLPHFVRRAETLFADKELVTATPAGRERTDYGTW
                     ADRTHRLGGVLDELGISEDGRVATFGWNTARHLELYFAAPCTGRVLHTLNIRLFPEQL
                     TYIANHAEDEVIFVDRSLLGLLMPLLPTFETVRHLVVMDDGAANEIPSNDGSDGPRIH
                     DYEDLIAAADPVAFQVDDERRAASMCYTSGTTGNPKGVVYSHRSTFLHTLGTMVAGGL
                     GVHEPDRILPVVPMFHANAWGLAHAAVAAGATLVMPGPDLSPPSLAALIEEEKVTVAA
                     GVPTIWMGVLPELKGRDTSALRAIPCGGSAVPRALSEAYREQTGLPILQAWGMTETSP
                     IASIATIKSTLDAELDDDGRADLRATVGQPSVCVTARIARPSAPGSPIEELPWDGESS
                     GELQVSGPWIASEYYDDERSPESFTDDGWLKTGDVAVIDGHGYIRLVDRTKDVIKSGG
                     EWISSVDLENELMGHPAVAEAAVIGIPHEKWAERPLACVVLKEGESATKEDLLAYLEG
                     RVAKWWLPDDIAFIDEVPKTSVGKFSKKDLRSKFEGYSLPTDA"
     gene            complement(3968..4384)
                     /locus_tag="VLR27_09945"
     CDS             complement(3968..4384)
                     /locus_tag="VLR27_09945"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP03812.1"
                     /translation="MAFHPYLNFNGTCREAFTRYQEIFGGELYVMGADDMPDGESVTP
                     DWNPDMVVHAALSLGDDMLMASDAPGSAPPASTYVHYEAKDVDDAKRVFEALAEGGEV
                     EMAMEETFWSPAFGSCKDRWGTLWLVGAPSDFEMPG"
     gene            complement(4460..>4822)
                     /locus_tag="VLR27_09950"
     CDS             complement(4460..>4822)
                     /locus_tag="VLR27_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSP03813.1"
                     /translation="SINHHESWGGPSWNHADHRAVGRALLDAVRDAANPWVFPDRGEA
                     WDGVRFTAFNASPQATHFVDVTDTFDAGVESLQCHATYLRHLEGDMADPEAFLRGGAE
                     EAGRAAGVGLATTFEVIG"
BASE COUNT          693 a         1787 c         1675 g          667 t
ORIGIN      
        1 gggccgggtg tcgatggtcg tcgacgacga gcacccgccg tactccttcg tgaaggtgga
       61 cggcacggtc accctgtccg aggacctgga cgaggtccgc cgcttcgcca ccatcatcgg
      121 cggtcgctac atgggcgcgg atctggccga ggagttcggc gcccgcaacg gcgtgccggg
      181 tgagctgctc gtgcgcgtgc acccgacccg ggtgctcgcc gcgtacgaca tcgccgactg
      241 agtcggtccg gctgctggcc cgatgggacc agccagcgat gcggggatgt cccggttcgc
      301 gcatggtgca actgcaccat gactagtccg aacgagggat ttcgccaacc acccggcgcg
      361 aagcctcaag tcggcccgcg cccacccccg atgtaggccc tgcggcgggc gtcgcccgcc
      421 gggacgggaa cgacaggggg agaggcgaga tgaacaagat ccgcatcgca ggcgcggtcg
      481 caggcacggc gctggtgtcc gggatggtgc tgcaggtgtc ctcggcagcg ttcagcgaca
      541 cgaccgagaa cccgggcaac agctgggcgg cggggaccgt cgcgctgtcc gacagccgca
      601 ccggctcggc gatgttcgcg gccacgaaca tggtcccggg cgacagtgag agccagtgca
      661 tcgacgtcac ctacgacggc tcggtcacgc cctcggaggc gattgcactc cacgccgagg
      721 tgaccgagag cgcggatgcc gtcggcaacg gcctcggtga cgacctcgac gtcaccgtcg
      781 agatgggccc ggccgacacg gactgcacga ccagcgcggc cggcgcgggg acgtcgatct
      841 acaccggcac gctcgccggc ttccacgctg ctgccgaccc gctcgccacc gggtggaccc
      901 cggacgccgc tgacggcgca gccgacatca gccgaccctt ctggttcacc atcaccctcg
      961 gcgacgacac gccgaacgac gcccagggcg acggggccac ggccgccttc acctggtcgg
     1021 ccacgagctg atcggccacc ggaccagccc accacgggat cgtcgtgacc gcgaccaccc
     1081 tccccacccc cgcagggacg gcagccggag ctggctggct gccgtccctg gccgggcagg
     1141 tgctggagtt cgtctggccg atgttcctca ccctggtcct cacgctcggg gcgtgggtgc
     1201 tgggcccgac cctgctcctc ggatggcagc cggtcgccat catctccggc tcgatggcgc
     1261 cggcggtccc gcccggccac gtcgtgctgg tggagccgtt ccggggtcag gacgtcggcc
     1321 cgggcaccgt cgtcacgtac cgcgacccgc gtcaggaccg cctcgtgacc caccgcatct
     1381 cggcggtggc ggaggacggg acgatcacga cccgcggcga cagcagcgtg gtcgacgacc
     1441 ccgtcccgct cagcaccgac cggatcgtgg gcgtcgggcg cctggtcgtc cccgccgccg
     1501 ggctgccggc gctgtgggcg cacgacggcc gggtcgatct gctcgccggc gtcgccgtcc
     1561 tgacggtgct gaccttcggc tcggccgcca gcgccgccgc aaccggggtc cgctccctcc
     1621 ggtcgcgccg gttgtcgctc cgacgtggcg cccgaccacc cgccagcccg acggtcgggc
     1681 tggtgaccgg cgtcgccagc gtcctggccg tgctcgccac cagcgcggtg tccgtcgccg
     1741 cgttcgtcgg cgagggccag aacccggcca acaccttccg ggccgggacc gtaccggcgc
     1801 cgaccatcac cgcggccacc gacgcctgcc cggcagcgga cccgaacgcc aggggcggca
     1861 agcgggcgat cgagctcgcc tgggacgcca tcccgaacgc gtcggcctac gaggtcgagc
     1921 gagcgaccga ccccgccggc ccgttcacgc ccctgggcac gatcccggac acctgcatgc
     1981 gggacaccga cgtgaacgcg gcgaccacgt accactaccg ggtccgcgcc catgcgggtc
     2041 cgtggaccag tgcatggggc accacctcgg ccgagctccc tcgcggaccc cgctgaaccg
     2101 gacgtgatcg atcgcgaccc cgtcggggta gggggtgcgg cgtgttcttc ttcttctcga
     2161 accgtctcgg ctgcctcggc tcgctcatgc tctcggtggt gctgtcgctg atcctcatcg
     2221 cgatcctgcg cgccctgtag cccgcagccc acccgcgccc cgctaccttc cccgccgaca
     2281 acacggggac gacgaccaca gggggacacc gtggacggac tcatgcagga cgcgccgctc
     2341 acgctgccgc acttcgtgcg gcgcgccgag acgctcttcg ccgacaagga gctggtcacg
     2401 gccacgcccg ccgggcgcga gcgcacggac tacggcacct gggccgaccg cacgcaccgc
     2461 ctcggcgggg tgctcgacga gctcggcatc tccgaggacg gccgggtcgc caccttcgga
     2521 tggaacaccg cccgccacct cgagctgtac ttcgccgcgc cgtgcaccgg ccgggtgctg
     2581 cacacgctga acatccgcct gttccccgag cagctcacct acatcgccaa ccacgccgag
     2641 gacgaggtca tcttcgtcga ccggtcgctg ctcggcctgc tgatgccgct gctgccgacg
     2701 ttcgagacgg tgcgccacct cgtcgtcatg gacgacggcg cggcgaacga gatcccgagc
     2761 aacgacggct ccgacggtcc ccgcatccac gactacgagg acctgatcgc cgcagccgac
     2821 ccggtcgcgt tccaggtcga cgacgaacga cgtgccgcga gcatgtgcta cacgtccggc
     2881 acgacgggca accccaaggg cgtcgtgtac tcgcaccgct ccacgttcct gcacacgctc
     2941 gggacgatgg tggcaggcgg gctcggtgtg cacgagccgg accgcatcct gccggtggtg
     3001 ccgatgttcc acgccaacgc ctggggcctg gcgcacgccg ccgtggccgc cggcgccacg
     3061 ctggtcatgc cgggacccga cctgtcgccg ccctcgctcg ccgcgctcat cgaggaggag
     3121 aaggtcaccg tcgccgccgg cgtgcccacc atctggatgg gcgtgctgcc cgagctgaag
     3181 ggccgggaca cctcagcgct gcgggccatc ccgtgcggcg gctcggccgt gccccgggcg
     3241 ctgtcggagg cctaccgcga gcagaccggc ctgccgatcc tgcaggcgtg gggcatgacg
     3301 gagacctccc ccatcgcgtc gatcgccacc atcaagtcca ccctcgacgc cgagctggac
     3361 gatgacggcc gggccgacct gcgggcgacc gtcggtcagc cgtcggtgtg cgtcaccgcc
     3421 cgcatcgccc gcccgtccgc gcccggctcg ccgatcgagg agctgccgtg ggacggggag
     3481 tcctccggcg agctgcaggt gtccggcccc tggatcgcca gcgagtacta cgacgacgag
     3541 cgctcacctg agtcgttcac cgacgacggc tggctcaaga ccggtgacgt ggcggtgatc
     3601 gacggccacg gctacatccg cctggtcgac cgcaccaagg acgtcatcaa gtccggtggc
     3661 gagtggatct cctcggtcga cctggagaac gagctgatgg gccaccccgc cgtggccgag
     3721 gccgcggtca tcggcatccc gcacgagaag tgggccgagc gaccgctggc gtgcgtggtg
     3781 ctgaaggagg gcgagtcggc caccaaggag gacctgctcg cctacctcga gggccgggtc
     3841 gccaagtggt ggctccccga cgacatcgcc ttcatcgacg aggtgccgaa gacgtcggtg
     3901 ggcaagttct ccaagaagga cctgcgctcc aagttcgagg gctacagcct gcccaccgac
     3961 gcgtagctca gcccggcatc tcgaagtcgc tcggggcacc gaccagccac agggtccccc
     4021 agcggtcctt gcacgaaccg aacgcgggcg accagaaggt ctcttccatc gccatctcga
     4081 cctcgccgcc ctcggcgagg gcctcgaaga cgcgcttcgc gtcgtcgacg tccttggcct
     4141 cgtagtgcac gtacgtgctc gccggcggcg cgctcccggg ggcatccgag gccatgagca
     4201 tgtcgtcgcc gagcgagagc gccgcgtgca cgaccatgtc cgggttccag tcgggggtga
     4261 cgctctcgcc gtcgggcatg tcgtcggcgc ccatgacgta gagctcgccg ccgaagatct
     4321 cctggtagcg ggtgaacgcc tcgcggcagg tgccgttgaa gttgaggtac gggtggaagg
     4381 ccatggtggg ctcctcggtc aggtggtggg ttcgattctc cgcgaccgac gggacggacg
     4441 ccacggattc atcggtggct cagccgatga cctcgaacgt ggtggccagg ccgacccccg
     4501 ccgcccgacc ggcctcctcg gccccgccgc gcaggaacgc ctccgggtcc gccatgtcgc
     4561 cctcgaggtg gcgcaggtag gtggcgtggc actggagcga ttcgacgccg gcatcgaagg
     4621 tgtcggtgac gtcgacgaag tgggtggcct gcgggctggc gttgaacgcg gtgaagcgga
     4681 cgccgtccca ggcctccccc cggtcgggga acacccacgg gttggcggcg tcgcgcacgg
     4741 cgtcgagcag ggcccggccc acggcgcggt ggtcggcgtg gttccaggac gggccgcccc
     4801 aggactcgtg gtggttgatc ga
//
LOCUS       DATISU010000248         5810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_153725, whole genome shotgun sequence.
ACCESSION   DATISU010000248 DATISU010000000
VERSION     DATISU010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153725"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..676
                     /locus_tag="VLR27_09955"
     CDS             74..676
                     /locus_tag="VLR27_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HSP03814.1"
                     /translation="MGWQLDKGLEGLDHNVLTGRVEDLVQWARKSSLWPATFGLACCA
                     IEMMAAGGAHYDMSRFGMEVFRASPRQADLMIVAGRVSQKMAPVLRNVYDQMMEPKWV
                     LSMGVCASSGGMFNNYAIVQGVDQIVPVDAYAPGCPPGPETLIHAINTIHEQIQSGEL
                     LRRRAETGGGAGVQLPQLDGQRAEGNRPVGTPVVLGEVRS"
     gene            673..1218
                     /locus_tag="VLR27_09960"
     CDS             673..1218
                     /locus_tag="VLR27_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSP03815.1"
                     /translation="MSDATPATEEHDEVVDDTTPEPEELYGQSVTWSRGQAVIHVGRD
                     QLQDVVRTLRDDEGFNVCIDVTAVDYLTYEAPRDVPAGIDAERFEVVVNLLSHEHRSR
                     VRIRVQVPEHDPSCPTLFEVHPGTEAFEREAYDMFGIRFAGHPDLSRILMPEEWEGHP
                     LRKDYAVGRIPVQFKGVVNER"
     gene            1215..2549
                     /locus_tag="VLR27_09965"
     CDS             1215..2549
                     /locus_tag="VLR27_09965"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HSP03816.1"
                     /translation="MSTSSETEMAFTNEGSQEMSTRASGTPGELLRELGATLRMTEAE
                     AAALIDEEAEEGSTMIINMGPQHPSTHGVLRLMLELEGEKVLRTKTIIGYLHTGMEKT
                     GEDLTFLQGPTNVTRMDYAAPLFNELVFSLATEKLLGIDEDIPERAQWIRMLLCELNR
                     MTSHLLFMATNGMDVGAVGMMIYGWREREEGLRLLEKITGLRMNHNFIRPGGVAADLP
                     DGWRDDVLHLLDYLPDQLDEFDTLLTNQPIYRERLQGVGVITQQEAIALGATGPVLRA
                     TGLARDLRRDEPYLRYDQVDFDVLVGSYGDSFDRYAIRLNEIRESMKIVRQILDRMPA
                     GDYRIQDKKVTPPPRARIDESMEALIHHFKIFTEGFKVPAGEVYVPIESPRGELGCYL
                     VSDGSSKPYRMHISGPSFRNLQTLPHMMRDGLIADGVVIISSVDPIMGEVDR"
     gene            2621..4393
                     /locus_tag="VLR27_09970"
     CDS             2621..4393
                     /locus_tag="VLR27_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP03817.1"
                     /translation="MSTPDARVRHPASANLRRVGPYFAPHRGRMWFVGLSAVVSIAAG
                     LSTPLIAKAVIDGPIARGDKAGIVPWVVLAVVLGLVETGLARVRRVKLAVAAMDVETK
                     LRNDLYRHLQRLDVGFHDRWQSGQLLSRATSDIATIRRFTAFGAVFLLIISLEVVVIF
                     ALLVSLYWPLAIVAGAFAVPVLVLCARFEVQYTAIVRRIQDETGDMTTEIEEAAKGIG
                     VIKAFGRARFAFDRYDTHVRRLYASQMERITVHTKFVWLLGIVPNLILVATLLAGVLA
                     VGSGDLTLGGLFAFVSYILILVFPLEALGWILAMAQEAMTAADRVYDVIDTPPVVDDR
                     PDAVELPEVHGHIRFEGVRFAYGEDREVLRGVDLEIAPGETLALVGPTGSGKSTLAML
                     LARLHDPTAGRVTVDGHDLRDVTLASLRGQVSVAFEEATLFSASVRENLRIGRPDATE
                     ADIEEALDVARAGFAHDLPWGLDTRIGEQGMTLSGGQRQRLALARAVIAKPRILVLDD
                     PLSALDVHTEAEVEARLRPLLADRTALVVVHRPSTISLADRAALLIDGRIVATGTHTE
                     LLSDEPRYREILAQGAEDEEGAHA"
     gene            4390..>5810
                     /locus_tag="VLR27_09975"
     CDS             4390..>5810
                     /locus_tag="VLR27_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HSP03818.1"
                     /translation="MTLHPLEDEGHEHDPIGIDLVGIDVTGDEAIDSWRGVAAEDADD
                     VEGSLATLLRSRSRRLLGSLVAPHRRAMGWATVLIAVNMVAHLSGPWFVGRAIDDGIP
                     PLLEGGSGSLRPLLTVVGGYLVATVVAAATFNAFLLVSGRIGQEVIVDIRRRLSRHFT
                     TLSLSFHQRYTSGRVISRQTSDVDAIAELLGHGLLQLTTSSLTLVGITIILVVLDPLL
                     ALAALSVVPLLWLLTRWFRHHSEKAYRATREAIALVIVHFVESLGGIQAVQSFRRERR
                     NQEIFDDLDGRYRDANVWSARLAATYGPGVQFAGRATVAVVLLYGGTRVIDGAIEIGV
                     LASFVLYLRRFFEPMTELSQFYNLFQAAAAATEKLSGVLEEEPTVAPPTDAVPLPEAA
                     GAVAFEGVSFAYGDEVVLHGVDLVVPAGQTVAVVGETGAGKSTIARLTARFWDPTEGS
                     VRLDGIPLTRLDEEDLRRAVVVVT"
BASE COUNT          855 a         2174 c         1930 g          851 t
ORIGIN      
        1 gggtcggcct cgagggccga cccaccagca cgtccgaagt cgcctgaccg aagagccgag
       61 agaggagtcc cccatggggt ggcagctcga caagggcctc gaagggctcg atcacaacgt
      121 gctcaccgga cgggtggagg atctggtcca gtgggcccgc aagtccagcc tgtggccggc
      181 cacgttcggc ctggcctgct gcgccatcga gatgatggcg gccggcggcg cgcactacga
      241 catgtcccgc ttcggcatgg aggtgttccg cgcctcgccc cgccaggccg acctcatgat
      301 cgtcgccggc cgggtcagcc agaagatggc cccggtgctg cgcaacgtct acgaccagat
      361 gatggagccc aagtgggtgc tgtcgatggg cgtctgcgcc agctcgggcg gcatgttcaa
      421 caactacgcc atcgtgcagg gcgtcgacca gatcgtcccc gtcgatgcct acgcgcccgg
      481 ctgccccccc ggccccgaga ccctgatcca cgcgatcaac acgatccacg agcagatcca
      541 gagcggcgag ctgctccgac gccgggccga gaccggcggc ggcgccggtg tccagctgcc
      601 ccagctcgac ggtcagcgtg ccgagggcaa ccgcccggtc ggcacccccg tcgtcctcgg
      661 ggaggtccgg tcgtgagcga cgcgaccccg gccaccgagg agcacgacga ggtcgtcgac
      721 gacaccaccc ccgagcccga ggagctctac gggcagtcgg tcacgtggtc ccgcggccag
      781 gccgtgatcc acgtcggtcg cgaccagctc caggacgtcg tgcgcacgct gcgcgacgac
      841 gagggcttca acgtctgcat cgacgtcacc gctgtggact acctgaccta cgaggcgccg
      901 cgcgacgtcc ccgccgggat cgacgccgag cgcttcgagg tcgtggtcaa cctgctcagc
      961 cacgagcacc gcagccgggt ccgcatccgg gtgcaggtcc ccgagcacga cccgtcctgc
     1021 cccaccctct tcgaggtcca cccgggcacc gaggccttcg agcgcgaggc ctacgacatg
     1081 ttcggcatcc gcttcgccgg ccaccccgac ctcagccgca tcctgatgcc cgaggagtgg
     1141 gagggccacc cgctgcgcaa ggactacgcc gtcgggcgca tccccgtgca gttcaagggc
     1201 gtggtgaacg agcgatgagc acctccagcg agaccgagat ggccttcacc aacgaaggca
     1261 gccaggagat gtcgacgcgc gcgtcgggca cccctggcga gctgctccgc gagctcggcg
     1321 ccaccctgcg catgaccgag gccgaggccg ccgccctcat cgacgaggag gccgaagagg
     1381 gctcgaccat gatcatcaac atgggtccgc agcacccgtc cacccacggg gtgctgcgcc
     1441 tcatgctgga gctcgagggc gagaaggtcc tgcgcaccaa gacgatcatc gggtacctcc
     1501 acaccggcat ggagaagacc ggcgaggacc tcaccttcct gcagggcccc acgaacgtca
     1561 cccgcatgga ctacgcggcg ccgctgttca acgagctggt gttctccctc gccaccgaga
     1621 agctgctcgg catcgacgag gacatcccgg agcgggccca gtggatccgg atgctcctgt
     1681 gcgagctgaa ccggatgacg tcgcacctgc tgttcatggc caccaacggc atggacgtcg
     1741 gcgcggtcgg gatgatgatc tacggctggc gcgagcgcga ggagggcctg cgcctgctcg
     1801 agaagatcac cggcctgcgg atgaaccaca acttcatccg gcccggcggt gtcgccgccg
     1861 acctgcccga cggctggcgc gacgacgtcc tccacctgct cgactacctg cccgaccagc
     1921 tcgacgagtt cgacacgctg ctgaccaacc agccgatcta ccgcgagcgc ctccagggcg
     1981 tcggcgtgat cacccagcag gaggccatcg cgctcggcgc caccggcccg gtgctgcgcg
     2041 ccaccggcct cgcccgagac ctgcgcaggg acgagcccta cctgcgctac gaccaggtcg
     2101 acttcgacgt gctcgtgggc tcctacggcg acagcttcga ccgctacgcc atccgcctca
     2161 acgagatccg cgagtcgatg aagatcgtcc gccagatcct cgaccgcatg cccgccggcg
     2221 actaccggat ccaggacaag aaggtcaccc cgccgccgcg cgctcgcatc gacgagtcga
     2281 tggaggccct catccaccac ttcaagatct tcaccgaggg cttcaaggtg cccgccggcg
     2341 aggtctacgt gccgatcgag agcccccggg gcgagctcgg ctgctacctg gtctcggacg
     2401 gctcgtcgaa gccgtaccgc atgcacatct ccgggcccag cttccgcaac ctgcagacgc
     2461 tgccccacat gatgcgcgac ggcctcatcg ctgacggcgt ggtgatcatc tcctcggtcg
     2521 accccatcat gggcgaggtc gaccgctaga tattcgggcg caagcgggcg atctgcccgc
     2581 ttagggtcct cgcccggcgc cggagtccgg gaggaggacc atgtcgacac cagacgcgcg
     2641 cgtgcgccat ccggccagcg ccaacctccg ccgggtcggg ccctacttcg caccccaccg
     2701 ggggcggatg tggttcgtcg gtctgtcggc cgtcgtctcc atcgcggccg ggctctcgac
     2761 gccgctcatc gccaaggcgg tcatcgacgg gcccatcgca cgcggcgaca aggccggcat
     2821 cgtgccctgg gtcgtgctcg ccgtcgtcct cgggctggtc gagaccgggc tggcgcgcgt
     2881 gcggcgggtg aagctcgcgg tggcggccat ggacgtcgag acgaagctgc gcaacgacct
     2941 gtaccggcac ctgcagcggc tcgacgtcgg gttccacgac cgatggcagt ccggccagct
     3001 gctgtcgcgc gccaccagcg acatcgccac catccgtcgc ttcacggcct tcggtgcggt
     3061 gttcctgctg atcatcagcc tcgaggtggt ggtgatcttc gccctcctcg tgagcctcta
     3121 ctggcccctc gccatcgtgg cgggggcctt cgccgtgccg gtcctggtgc tctgcgcccg
     3181 cttcgaggtc cagtacaccg ccatcgtccg gcgcatccag gacgagaccg gggacatgac
     3241 caccgagatc gaggaggccg ccaagggcat cggcgtcatc aaggccttcg gccgggcccg
     3301 cttcgccttc gaccgctacg acacccacgt ccgccggctg tacgcctccc agatggagcg
     3361 gatcaccgtc cacacgaagt tcgtgtggct gctcggcatc gtgccgaacc tgatcctggt
     3421 ggccacgctg ctcgccggcg tgctggcggt cgggtccggc gacctcacgc tcgggggact
     3481 gttcgccttc gtctcctaca tcctgatcct ggtcttcccc ctcgaggccc tcggctggat
     3541 cctggccatg gcccaggagg ccatgaccgc cgccgaccgg gtgtacgacg tcatcgacac
     3601 cccacccgtc gtcgatgacc gccccgatgc ggtggagctc cccgaggtgc acgggcacat
     3661 ccgcttcgag ggagtccggt tcgcgtacgg cgaggatcgg gaggtgctgc gcggcgtgga
     3721 cctcgagatc gcgccggggg agacgctggc gctcgtcggt cccaccggtt cgggcaagag
     3781 cacgctggcg atgctgctgg cccggctgca cgaccccacc gcgggccggg tcaccgtcga
     3841 cggccacgat ctccgcgacg tgacgctggc cagcctccgt gggcaggtca gcgtggcgtt
     3901 cgaggaggcc acgctgttct cggccagcgt gcgggagaac ctgcgcatcg ggcggcccga
     3961 cgccaccgag gccgacatcg aagaggccct cgacgtggcg cgggccggct tcgcccacga
     4021 cctgccctgg ggcctcgaca cccgcatcgg cgagcagggc atgaccctgt cgggcgggca
     4081 gcgccagcgc ctggcgctgg cccgggccgt catcgccaag ccccggatcc tggtcctcga
     4141 cgacccgctc tcggccctcg acgtccacac cgaggccgag gtcgaggcgc ggctgcgacc
     4201 cctcctcgcc gaccggaccg cgctcgtggt cgtgcaccga ccctccacca tctccctggc
     4261 cgaccgggcc gcgctgctga tcgacggccg gatcgtggcc acgggcaccc acaccgagct
     4321 gctcagcgac gagccccgct accgggagat cctcgcccag ggcgccgagg acgaggaagg
     4381 agcgcacgca tgacgctgca ccccctcgag gacgaaggcc acgagcacga cccgatcggg
     4441 atcgacctgg tcggcataga cgtcaccggc gacgaggcga tcgactcgtg gcgcggcgtc
     4501 gcggccgagg acgccgacga cgtcgagggc agcctggcca ccctgctgcg gtcgcgcagc
     4561 cggcgcctcc tcggctcgct cgtggccccg caccggcgcg ccatgggctg ggccacggtc
     4621 ctgatcgccg tcaacatggt cgcccacctc tccgggccct ggttcgtcgg ccgggccatc
     4681 gacgacggga tccccccgct gctcgagggc gggagcggct cgctgcggcc gctgctcacc
     4741 gtcgtcggcg gctacctggt ggcgaccgtg gtcgccgcgg ccaccttcaa cgcgttcctg
     4801 ctcgtgtccg gccgcatcgg ccaggaggtc atcgtcgaca tccgtcggcg cctgtcccgg
     4861 cacttcacga cgctcagcct gagcttccac cagcgctaca cgtcgggtcg ggtgatctca
     4921 cgccagacca gcgacgtcga cgccatcgcc gagctgctcg ggcacggcct gctgcagctg
     4981 accacctcgt ccctgacgct cgtcggcatc acgatcatcc tcgtcgtcct cgacccgctg
     5041 ctggcgctgg ccgccctgtc cgtggtcccg ctcctgtggc tgctgacccg ctggttccgg
     5101 caccactcgg agaaggcgta ccgcgccacc cgggaggcca tcgccctcgt catcgtccac
     5161 ttcgtcgagt ccctcggcgg catccaggcg gtgcagtcgt tccgccggga gcgccgcaac
     5221 caggagatct tcgacgacct cgacggtcgc taccgcgacg ccaacgtgtg gtcggcacgc
     5281 ctcgccgcca cctacggccc cggcgtgcag ttcgccggcc gggccacggt cgccgtcgtg
     5341 ctgctctacg gcggcacccg ggtcatcgac ggcgccatcg agatcggcgt gctggcctcg
     5401 ttcgtgctct acctgcgccg gttcttcgag ccgatgaccg agctgagcca gttctacaac
     5461 ctgttccagg ctgcggccgc cgccaccgag aagctgtcgg gggtgctcga ggaggagccc
     5521 accgtcgcac cgccgaccga cgcggtgccg ctgcccgaag cggccggtgc ggtcgcattc
     5581 gagggcgtca gcttcgccta cggcgacgag gtggtcctgc acggcgtgga tctcgtcgtc
     5641 cccgccggtc agaccgtggc ggtggtgggg gagacgggtg cgggcaagtc caccatcgcc
     5701 cgcctcaccg cccggttctg ggacccgacc gagggctcgg tccgcctcga cgggatcccg
     5761 ctcacccgcc tggacgagga ggacctgcgc cgggccgtgg tcgtggtgac
//
LOCUS       DATISU010000249         1682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_619182, whole genome shotgun sequence.
ACCESSION   DATISU010000249 DATISU010000000
VERSION     DATISU010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619182"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1308)
                     /gene="proB"
                     /locus_tag="VLR27_09980"
     CDS             complement(199..1308)
                     /gene="proB"
                     /locus_tag="VLR27_09980"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HSP03819.1"
                     /translation="MSVGERGQIVVAKIGSSSITGDDGTINEAAIAKFCRDVAGLRAA
                     GHQVVMVTSGAIAAGLPAMDFDVRPRDAVTLQAVSAVGQSRLMAVYDRELAGHGLTAG
                     QVLLAPLDFMVREQYLHARATLGRLLDLGVVPVVNENDAIADDEIRFGDNDRLAALVA
                     HLIRADLLVLLTDAPGLLTADPRFDSTASLIEEILEVDHSLEELAGGPGSDRGSGGMA
                     SKLAAAKIAAWSGVRAVIAQASRSDVLTHAVDRTAGVGTVIQPRKQRLPARKLWIAFA
                     VGAAGTIVVDDGARRALLDRGVSLLPAGVVDVKGGFDVDAAVEIAGPDGRAFAKGLSR
                     FDAAGLRSVLGTRTEHLPEGSPHEVVHRDDLVVLP"
     gene            complement(1305..>1682)
                     /gene="cgtA"
                     /locus_tag="VLR27_09985"
     CDS             complement(1305..>1682)
                     /gene="cgtA"
                     /locus_tag="VLR27_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HSP03820.1"
                     /translation="DGTWEWDGERVTAVTGEGVPQLVGRMVQAVTDARQAMPEREAYV
                     VHRLAGEGYEVVRDETGTGWDVIGREAIRAVRLSDLTNPEALDEAHRRLKSLGIDRAL
                     ARAGAKHGDTVRIGKLEFDYEVE"
BASE COUNT          218 a          593 c          635 g          236 t
ORIGIN      
        1 tcgctccgga cgccgctgag gcggagcccg aggccatcgc cggcgagtcc gctcccgagg
       61 gtcagaccca ggccgagggc cagactcaac cctcggacgg caccgcttct tgaaactggc
      121 agccgcctcg tcacacgaag cccgtgtgtg acgattcggc tgccagtttc ctctccactt
      181 gcgctggccg cttccccctt acgggagtac cacgaggtcg tctcggtgga cgacctcgtg
      241 gggggagccc tcggggaggt gctcggtcct cgtgccgagg accgagcgca ggccggcggc
      301 gtcgaagcgg ctgaggccct tggcgaaggc ccggccgtcc gggccggcga tctcgacggc
      361 agcgtcgacg tcgaagccgc ccttcacgtc gaccacgccg gcggggagga gcgagacgcc
      421 gcggtcgagc agggctcggc gggcgccgtc gtcgaccacg atcgtcccgg ctgcgccgac
      481 ggcgaaggcg atccacagct tgcgggccgg caggcgctgc ttgcggggct ggatgaccgt
      541 gccgacgccg gcggtgcgat ccacggcgtg ggtgagcacg tcgcttcgcg acgcctgggc
      601 gatgacggca cggacgccgg accaggcagc gatcttggcg gccgccagct tggaggccat
      661 gccaccgctg ccccggtcgc tcccgggtcc gccggccagc tcctcgaggc tgtggtcgac
      721 ctcgaggatc tcctcgatga gcgaggcggt gctgtcgaag cggggatcgg cggtgagcag
      781 gccgggggca tcggtgagca ggacgagcag gtcggcccgg atgaggtggg ccacgagggc
      841 ggccaggcgg tcgttgtcgc cgaagcggat ctcatcgtcg gcgatcgcgt cgttctcgtt
      901 caccaccggt acgacgccga ggtccagcag ccggccgagc gtggcccggg cgtgcaggta
      961 ctgctcacgc accatgaagt cgagcggcgc cagcaggacc tgcccggcgg tgaggccgtg
     1021 gccggcgagc tcccggtcgt agaccgccat cagccggctc tgccccacgg ccgacacggc
     1081 ctggagggtg acggcgtccc gggggcgcac gtcgaagtcc atggccggca ggccggcggc
     1141 gatggccccg gaggtcacca tgaccacctg gtgcccggcg gcgcggagcc cggcgacgtc
     1201 gcggcagaac ttggcgatcg ccgcctcgtt gatggtcccg tcgtcgcccg tgatcgacga
     1261 cgagccgatc ttggcgacga cgatctggcc ccgctccccc acgctcactc gacctcgtag
     1321 tcgaactcga gcttgccgat gcgcacggtg tctccgtgct tcgcgccggc gcgggcgagg
     1381 gcccggtcga tgccgaggga cttcagccgg cggtgggcct cgtcgagggc ctcggggttg
     1441 gtgaggtccg acagccgcac ggcgcggatg gcctcgcggc cgatgacgtc ccagccggtg
     1501 ccggtctcgt cgcggacgac ctcgtagccc tcgcccgcca ggcggtggac gacgtaggcc
     1561 tcgcgctcgg gcatggcctg gcgggcgtcg gtgacggcct gcaccatgcg tccgacgagc
     1621 tggggcacgc cttcgccggt gacggcggtg acccgctcgc cgtcccactc ccaggtgccg
     1681 tc
//
LOCUS       DATISU010000250         3166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_479942, whole genome shotgun sequence.
ACCESSION   DATISU010000250 DATISU010000000
VERSION     DATISU010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479942"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VLR27_09990"
     CDS             <1..435
                     /locus_tag="VLR27_09990"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HSP03821.1"
                     /translation="PVVGICNGFQVLTEAGMLPGALQKNAGLKFLCRMATLRVETTDS
                     VLTSVAEQGAELRVPVNHFEGNYTCDDETLAMLRSEDRVVVRYVENVNGSVDDIAGVC
                     NEGRNVVGLMPHPERACDDLLGSSDGAPLLRSLLESASRARV"
     gene            complement(488..892)
                     /locus_tag="VLR27_09995"
     CDS             complement(488..892)
                     /locus_tag="VLR27_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03822.1"
                     /translation="MAPKREMTDEHKAALAEGRRQGRAVRDYLEALEQHKPKRGRKRT
                     AESMKAQLVKIEEQLVDADPVKRLQLIQDRMDLQNELEASENKADLEGLEADFVQAAK
                     GYSERKGISYAAWRELGVEASVLKKAGISRAS"
     gene            1049..>3166
                     /gene="purL"
                     /locus_tag="VLR27_10000"
     CDS             1049..>3166
                     /gene="purL"
                     /locus_tag="VLR27_10000"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HSP03823.1"
                     /translation="MAEQTEQDPVEQDPSVHRALGLTDDEAEEIARILGRPANHLELA
                     MYSVMWSEHCSYKSSRVHLRRLPSEAPHVLVGPGEGAGVVDVGDGIAAAFRIESHNHP
                     SAIEPYQGAATGVGGILRDVFSMGARPIALMDPLRFGPLDDPRSRWIAEGVVSGVSGY
                     GNSVGVPTVGGETIFDATYQGNPLVNVFCLGVLPQDRLALARASGPGNLTVLFGSATG
                     RDGIGGASVLASASFTEGDEEKRPSVQVGDPFEEKRLIEACLEILEAGLVVGIQDLGA
                     AGITGAASETSSKGGVGMDLYLDQVHVREEGMDPFEIMISESQERMLAIVEPAKLDQL
                     LAICAKWEIAASVIGTVTGTGRFRVLDRLDGDVLADIPASSLDDDAPLYQRPLAEPAD
                     RAERAADTAEALPAPDDAGADLLALLADTSWVWSQYDHQLFLNTVVGPGDDATVLRLK
                     DPRTGEPTGRGLGLTTDGNHRWCRLDPRTGMAAVVAESVMNLACVGAEPLALVNCLNF
                     GNPEHPEVMWQLSEAVDGMAEACRVLSVPVIGGNVSLYNESNGVNIDPSPVIGTVGLI
                     ASLERRPPGMGLVEGTTLVLLGTPATTLSGSAWAWSQDHREGMPPQVDLDGVRTTATF
                     VRDAVAGDRLRSVHDLAGGLGPALAEMAVATQVGARVDLPEPGGHVALFAETPAAVLA
                     AVDVGSVDALLADARAAGVPAVVI"
BASE COUNT          418 a         1174 c         1085 g          489 t
ORIGIN      
        1 ccggtcgtgg gcatctgcaa cggcttccag gtgctcaccg aggccggcat gctcccgggt
       61 gcgctgcaga agaacgccgg gctgaagttc ctgtgccgca tggcgaccct gcgggtcgag
      121 accaccgact ccgtgctgac gtcggtggcc gagcagggcg ccgaattgcg cgtcccggtc
      181 aaccacttcg agggcaacta cacctgcgac gacgagacgc tggccatgct ccggtccgag
      241 gatcgcgtcg tcgtgcgcta cgtggagaac gtcaacggat ccgtcgacga catcgccggt
      301 gtgtgcaacg aaggccgcaa cgtcgtgggc ctcatgccgc acccggagcg ggcatgtgac
      361 gacctgctcg gctccagcga cggtgcgccg ctgctgcgct cgctgctgga atccgccagc
      421 cgcgcccgcg tctgaccgct acaggagcac tgccagtttc cgatggccgt cggccggtgg
      481 ggcgggttca ggaagctcgg ctgatgccgg ccttcttcag caccgaggcc tcgacgccga
      541 gctcgcgcca ggcggcgtac gagatgccct tgcgctcgct gtagcccttg gctgcctgca
      601 cgaagtcggc ctcgaggccc tcgaggtcgg ccttgttctc gctggcctcg agctcgttct
      661 gcaggtccat gcggtcctgg atcagctgca ggcgcttgac cggatcggcg tcgaccagct
      721 gctcctcgat cttgaccaat tgggccttca tcgattcagc ggtgcgcttg cgcccgcgct
      781 ttggcttgtg ctgctcgagc gcctcgagat agtcgcgaac ggcgcggccc tggcggcgtc
      841 cttctgcgag tgcggccttg tgctcgtcgg tcatttcgcg cttgggtgcc atcgctctgc
      901 agggaaccac agatcgcgtc catatggcaa ttcatctctg ttccgggccg ccccatatcc
      961 gcatgtgcgg tgtcgcgctt tccgggatct ccgctcccgt catgtggacg tgcggggcgt
     1021 cggggcgggc tcgcgtagcg tgctcgccat ggccgagcag accgagcagg accccgtcga
     1081 gcaggacccg agcgtgcacc gggccctcgg tctgaccgac gacgaggccg aggagatcgc
     1141 ccgcatcctc ggccggccgg ccaaccacct cgagctggcc atgtattcgg tgatgtggtc
     1201 ggagcactgc tcctacaagt cctcacgcgt ccacctgcgc cggctgccgt cggaggcgcc
     1261 ccacgtcctc gtcgggccgg gcgagggtgc cggcgtggtc gacgtcggcg acggcatcgc
     1321 cgccgcgttc cgcatcgaga gccacaacca cccctccgcc atcgagccct accagggggc
     1381 ggccaccggg gtcggcggca tcctgcgcga tgtcttctcc atgggcgcac gtcccatcgc
     1441 cctgatggac ccgctgcggt tcggtcccct cgacgacccc cgcagccggt ggatcgccga
     1501 gggcgtggtg tccggcgtgt cgggctacgg caactccgtc ggcgtcccca cggtgggcgg
     1561 cgagacgatc ttcgacgcca cctaccaggg caacccgctg gtcaacgtgt tctgcctcgg
     1621 cgtgctgccc caggaccgcc tcgcgctggc ccgggcgtcg ggccccggca acctcaccgt
     1681 cctgttcggc tcggccacgg gtcgggacgg catcggcggc gccagcgtgc tcgcctcggc
     1741 ctcgttcacc gaaggcgacg aggagaagcg gcccagcgtc caggtcggcg acccgttcga
     1801 ggagaagcgg ctcatcgagg cgtgcctgga gatcctcgag gccggcctcg tcgtcggcat
     1861 ccaggacctc ggcgcggcgg gcatcaccgg agcggccagc gagacgtcgt cgaagggcgg
     1921 cgtcggcatg gacctgtacc tggaccaggt gcacgtccgg gaggagggga tggacccctt
     1981 cgagatcatg atcagcgaga gccaggagcg gatgctggcc atcgtcgagc cggccaagct
     2041 cgaccagctg ctcgccatct gtgccaagtg ggagatcgcc gcctcggtca tcggcacggt
     2101 caccggcacc ggccggttcc gggtgctcga ccgcctcgac ggcgacgtgc tcgccgacat
     2161 cccggccagc tccctcgacg acgacgcccc gctctaccag cgccccctcg ccgagccggc
     2221 cgaccgggcc gagcgcgccg ctgacaccgc cgaggcgctg cccgcgcccg acgacgccgg
     2281 cgccgacctg ctcgccctcc tggccgacac gtcctgggtc tggtcccagt acgaccacca
     2341 gctgttcctc aacaccgtgg tcggcccggg tgatgacgcc accgtcctgc ggctgaagga
     2401 cccccgcacc ggggagccca ccggccgagg cctcggcctc accaccgacg gaaaccaccg
     2461 ctggtgccgc ctcgaccccc gcaccggcat ggccgccgtg gtcgccgagt cggtcatgaa
     2521 cctggcctgc gtcggcgccg aacccctggc cctcgtgaac tgcctgaact tcggcaaccc
     2581 cgagcacccc gaggtcatgt ggcagctctc cgaggccgtc gacggcatgg ccgaggcgtg
     2641 ccgggtcctg tccgtcccgg tcatcggcgg caacgtgtcg ctctacaacg agtccaacgg
     2701 cgtgaacatc gacccgtcac cggtcatcgg caccgtcggc ctcatcgcct cgctcgagcg
     2761 ccggcccccg ggcatgggcc tggtcgaggg caccacgctg gtcctgctcg gcaccccggc
     2821 cacgaccctg tcgggcagcg cgtgggcgtg gtcgcaggac caccgcgagg gcatgccgcc
     2881 ccaggtcgac ctcgacgggg tgcggaccac cgcgacgttc gtgcgcgacg ccgtcgccgg
     2941 cgaccggctc cggtcggtgc acgacctcgc cggcggcctc gggccggcgc tggccgagat
     3001 ggccgtcgcc acccaggtcg gcgcccgggt cgacctgccg gagccgggcg ggcacgtcgc
     3061 gctcttcgcc gagacgccgg ccgcggtgct ggccgcggtc gacgtcggtt cagtcgatgc
     3121 cctgctcgcg gatgcccgtg cagcgggcgt gccggccgtg gtcatc
//
LOCUS       DATISU010000251        18603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_852720, whole genome shotgun sequence.
ACCESSION   DATISU010000251 DATISU010000000
VERSION     DATISU010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 18603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18603
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852720"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VLR27_10005"
     CDS             complement(<1..213)
                     /locus_tag="VLR27_10005"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HSP03824.1"
                     /translation="MTSPITVELWRGPHVESRHVVHAAVADVEGNVVRSWGDPDRLTH
                     PRSAVKPLQALPLIETGAADAFDLGPV"
     gene            complement(319..2076)
                     /locus_tag="VLR27_10010"
     CDS             complement(319..2076)
                     /locus_tag="VLR27_10010"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HSP03825.1"
                     /translation="MPGAGPLAGEAVAVVVSCTHRGSGYERRVGGPDPSPQTGRITEL
                     QTAATQPSPLFDALTWDDRFVRALPGDARSEVFPRQVPGAAWSPVRPTPVAAPRLRAW
                     SPEVAAPLGLPASPTPDEADEIARVLSGNAVVDGMTPRADRYGGHQFGNWAGQLGDGR
                     AIALGEVVDADGRNQTLQLKGAGPTPYSRSADGRAVLRSSVREFLCSEAMHHLGVPTT
                     RALSLVTTGDQVVRDMFYDGHPAPEPGAVVCRVAPSFTRFGTFELPASLGEEDLLRQL
                     VDHTLAHDFPHLGAPRDDQHRRELLIDLFDEVCRRTASLMVDWMRVGFVHGVMNTDNL
                     SILGLTIDYGPYGWLEPYDEGWTPNTTDKYMKRYRYGTQPMVAQWNLSRLGGAFHVLV
                     GDATMLEASLGSYAATIEQELPRAWLAKLGIEVDGVVAEPDRRLVADLHEVLSSTEID
                     MTIFFRSLPDVATLDAAATDRSLVEPLLGACYAPDEVTGDVLAGLSGWLRRWRERLDV
                     DDPRARMDAVNPKYVLRNYLAQLAIDAAEQDDDTLIAELHDTLRRPYDDQPGRERFAE
                     KRPEWARERAGCSMLSCSS"
     gene            2017..2955
                     /locus_tag="VLR27_10015"
     CDS             2017..2955
                     /locus_tag="VLR27_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HSP03826.1"
                     /translation="MGAGHDHGHGLAGQRAGTRHRRRLAISFGLIAVFFVVEAVAGVL
                     TNSLALLSDAGHMLTDVVGLGMALAAIQLADRHQAGAAHRSETSQHTFGLYRLEILAA
                     FVNALLLVGVAVYILVEAVRRIGGDPEIDTGPMLVVAVLGLLVNLVAFALLREGAKES
                     INVQGAYLEVVADTLGSVGVIVAAVLLELFGWTWVDPLVGALIGLWILPRTVRLGRQA
                     VRILLQSAPPSVDLGALRADLAALPDVLDVHDLHVWTLTSEMENATAHLVVEPGVDGH
                     HVLDQARALLQERYRIAHATLQVEPSDHTGCDELEW"
     gene            complement(3002..3817)
                     /locus_tag="VLR27_10020"
     CDS             complement(3002..3817)
                     /locus_tag="VLR27_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HSP03827.1"
                     /translation="MTTTTDPYADFDELTFDRPADGVLRITLDAPGLNSVSAAMHRQL
                     ADVWLAVDRDPETRVALLRGAGKGFSSGGSFDMIETIMDDPEFRLRAMREARDLVFNV
                     IGCSKPIVSAMHGPAVGAGLVAGILADISVVGRTARIIDGHTRLGVAAGDHAAICWPL
                     MAGMAKAKYYLLTCDTLTGEEAERIGLVSLCVEDEEVQDRAMEVAVKLANGAQSAIRW
                     TKHTLNHWFRAQSATFDASLAYEFLGFAGPDAREGLASHAEKRDPDFTGPTES"
     gene            complement(3801..4244)
                     /locus_tag="VLR27_10025"
     CDS             complement(3801..4244)
                     /locus_tag="VLR27_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03828.1"
                     /translation="MIRRLVAAVAAALLLVACGDEDTGRYDAQVDQVRQAVEAGDRAA
                     ALAGLEELGTDGFYAHAEGEITDEEVAELARLIEHARAQVDQELPEPTTTTESTTTTA
                     PPTTPPAPVFDDDDDDDKDDKDEKPDKKDRGNGRGGDDGDDDDDD"
     gene            complement(4241..5512)
                     /locus_tag="VLR27_10030"
     CDS             complement(4241..5512)
                     /locus_tag="VLR27_10030"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSP03829.1"
                     /translation="MTAGRADFPAVPLVRTDLAEPTVADRYELRSVLGSGGMGTVWEA
                     YDIRLGRTVALKVLRDDLPPSAAAQLEREARAAARITDPRVVTVLDLARTADGNPYMV
                     LECHDGHTLADELRDGPLPPERLEQLVDDLLGALGAAHLAGVLHRDVKPANVLANDGG
                     FRVTDFGLASLDDDQSTETDIMGTLVYVAPERLDGARGSTRSDVFSAAVVIHEAATGI
                     QPFRREQAVESIAALRTGDAPPLPETVPERLRQVLGLALSPDPDDRPEDAQSMLAAIR
                     SGAPLPVDPTVRLDATSVLPRSVTEPDPSEATVVVPAADDVAPDPARERTVTVPARPT
                     PDPAPAPAPAPQPHDDGPGPLEGVRSFLDRHGRRPEVIIGLVAALLILLLLLVVALDT
                     DPDPDPGTADAGQEAPASLDGTLDRIEELGR"
     gene            5571..6179
                     /gene="thrH"
                     /locus_tag="VLR27_10035"
     CDS             5571..6179
                     /gene="thrH"
                     /locus_tag="VLR27_10035"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HSP03830.1"
                     /translation="MADRQTIVTLDLEGVLVPEIWVAVADRTGIDALRRTTRDEPDYD
                     VLMRYRLDVLDEHGLTMSLVQDVIAGLEPLPGAKAFLDELRERTQVVILSDTFEQFAA
                     PLMRQLGMPTVFCHRLVVEDDRIVDYRLRQPDQKQCSVKAFQSLEYKVIAAGDSYNDT
                     TMLGAADAGFLFRAPENVIAEFPQFRAMTGYDELLDALTAEL"
     gene            complement(6163..7251)
                     /locus_tag="VLR27_10040"
     CDS             complement(6163..7251)
                     /locus_tag="VLR27_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HSP03831.1"
                     /translation="MTPAFDAILLLSFGGPEGPDDVVPFLENVTRGRGVPRERLEEVA
                     EQYLLFGGRSPINDQNKALLEALRAELDRRGQNLPLHWGNRNWVPFVEDVLGEITAAG
                     HRRVLAVSTSAYSSYSACRQYLDDIDRAVAAVRDAGGDPPAVEKVRPYWNHPGFVLAM
                     VERVHAALEALSAADRNRARLVFTAHSVPLSMAETSDYQAQLLDEATMIVERVAVPRI
                     GAARNGGADDDRGWDLVYQSRSGPPQVPWLEPDIVDHLGALHGRGEDVVIVVPIGFTS
                     DHMEVVYDLDTQAADAAKELGMTMVRAGTVGTHPLFVRGLVDVLEEHLVGRLPAALGA
                     LGPRVNPCRPGCCPAPARPVAGRPTARP"
     gene            7320..7913
                     /locus_tag="VLR27_10045"
     CDS             7320..7913
                     /locus_tag="VLR27_10045"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSP03832.1"
                     /translation="MELLLIRHALPIRVEGAAGPADPPLSDIGRRQSESMVRWLEPEP
                     LDALVVSPMIRARETATPLATARGLETVVHEGVAEFDKDADSYVPMEELKAAGDPRWQ
                     EIVDGGYFGDGDLTPEQFQATVVDAVEEVIAANAGGTAAIVCHGGVINAYVAHVLGID
                     DLLFFEPGYTSINRIRASRRGHRMVVSLNETAHLRDV"
     gene            7971..9185
                     /locus_tag="VLR27_10050"
     CDS             7971..9185
                     /locus_tag="VLR27_10050"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSP03833.1"
                     /translation="MHMKVDADAAGLDATRLGRIAEHLEQRYLEPGKIAGAQVAVLRH
                     GELGYFESFGQRDRERDQPVTEDTIWRWYSMTKPITGVALMTLYERGHFQLDDPVHRW
                     IPEWRDMQVRESDPDGGSRIVPAHRPPTVRDVLTHTSGIGYGPENRDLDLVTDRGFLG
                     GHDLESLGQAIGGWPLRFQPGTKWLYSFGMDVAARLVEVMSGLRFDEYLRTELFEPLG
                     MVDTGFSVPEDDLGRFAACYGRNSRKELVLVDDPERSSYRREPKLLNGGGGLVGTTGD
                     YLRFVAMLANGGELDGRRVISRKTLELMGSNHLPGDGDMADFALPMGYGEVGFSGAGF
                     GLTMAVSKGPAATGVVGSAGEMMWGGAASTTFWVDRAEELAVVFMTQLLPSGTFDFRN
                     QLKALVYGAIAD"
     gene            complement(9261..9875)
                     /locus_tag="VLR27_10055"
     CDS             complement(9261..9875)
                     /locus_tag="VLR27_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03834.1"
                     /translation="MPKNNTTTIRRRAAAVAACLLALGLAACGDDDDAAADETPTTEA
                     VDNETETETGDESDDAPEGEVVEVTAVDWAFEGLPASVEAGTKLSLTNEGEEPHELVA
                     MRIPDEETRPISELMALPEDELFPIFGDGEPAAVILAATGTTDTPGPVVGDGTLTEPG
                     RYAIVCFLPVGADDSILDPEADGPPESDAPPHVSQGMFAELTVS"
     gene            complement(9934..10446)
                     /locus_tag="VLR27_10060"
     CDS             complement(9934..10446)
                     /locus_tag="VLR27_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03835.1"
                     /translation="MVDVLILCTGNAARSVMAATSLSARRPDLRVATGGTFVVDGQPM
                     SIRTRAAIEGVGLEIPRHASRQVHPPDLDAATLVMALAPEHVEWVRRTHAPAARRTVT
                     LRRLVEHLVPAPTLTDQVAALAPHEVELEPWEEVVDPGGCEADVFAACAREIDALVHD
                     LAPRLAPPHP"
     gene            10510..11184
                     /locus_tag="VLR27_10065"
     CDS             10510..11184
                     /locus_tag="VLR27_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HSP03836.1"
                     /translation="MTQLLPLTADEVLATTRAVRKRLDFDRPVEDEVLRECLELALQA
                     PSGSNAQSWRFVVVTDPDKKQALGELYRQAFDIYEQLDGINAATIYRGDDPERLEQQQ
                     RVMGSARYLAERMGEVPAMLVPCMPGRSDGAPAVSAAAAYGSILPAVWSFMLAGRARG
                     LGTSWTTLHLMFEQQAAEILGIPFEEWTQVALVSVGYTKGPDFKRATREPLETAVSFN
                     GWDFED"
     gene            complement(11363..11563)
                     /locus_tag="VLR27_10070"
     CDS             complement(11363..11563)
                     /locus_tag="VLR27_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HSP03837.1"
                     /translation="MATGTVKFFNAEKGFGFISREDGDDVFVHFSNIAGEGFKSLDEG
                     QQVEFDIGPGRKGDEAHNVRAL"
     gene            complement(11672..12877)
                     /gene="menC"
                     /locus_tag="VLR27_10075"
     CDS             complement(11672..12877)
                     /gene="menC"
                     /locus_tag="VLR27_10075"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HSP03838.1"
                     /translation="MTGPDPTRSAADRNTTTASSGGALPGIDIGSVELRRIELPLVTP
                     FQTSFGTWTFRDILLVRVLAEGPDGPVEGWGECVAGTQPLYSSEYTSAAEDVTVDHLL
                     PRLFAAGVRHADDVAGALQEVRGHRMAKAAVEAAVLDAELRAAGRSMAAHLGAAVDRV
                     PVGVSIGIVPTIDDLVATAARHLDEGYARIKVKIKPGWDVEPCRRLRAELGDDFGLQV
                     DANAAYGPGDVDTLRALDEFGLLLIEQPYPEERIVDNVDLVQALQTPVCLDETIVDEA
                     VCDDVLRLGAADVINIKPGRVGGHLAGVRIHDACLAAGVPVWHGGMLETGIGRAPNLA
                     LAALPGFTLPGDISAAARYWETDVVTQPAVLEPDGTIRVPQGPGIGVEVDLDVLASVT
                     TTATLHHAD"
     gene            complement(12874..13668)
                     /locus_tag="VLR27_10080"
     CDS             complement(12874..13668)
                     /locus_tag="VLR27_10080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP03839.1"
                     /translation="MSAAAAQYEIRTVTGAEADRALDLAESVWGQRPLNDAVLRALEL
                     AGSYVSVALDGSGRQIGMCLGIVGVHDDHLHLHSHLAAVDPAHRGAGIGRALKRHQRE
                     WCLERGITTISWTFDPLIHANARFNLHHLGARGERYLVELYGHMEDEINRGDASDRLL
                     IRWDLDDERTRIALDRPLPTPDVAHADVALADDGGRPRRVDTDAAVRLIATPADAVAL
                     RRDDPEMARAWRLAVRETMLQAFAEGLEPVAVTTDRRYLFTRPEDR"
     gene            complement(13698..15512)
                     /locus_tag="VLR27_10085"
     CDS             complement(13698..15512)
                     /locus_tag="VLR27_10085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HSP03840.1"
                     /translation="MSTETHAPEVTGAEGVAWDIGTLVDGEGADGVRRLLDEAIESAR
                     ALTADVKGTLTDADASTLSAALDRLAEIYEQARRAGSYASLDFAVDTSDPARGALMQE
                     VSERSAALSAELVWFDLEVVAIDDDRAAQLLDDPALAEHRHHLETLRAGKPHVLSEVE
                     ERLLANTAPTGRQAWVRLFTEQTSSIQVDLPDEPEPVPLDAGLSRLLDPDRATRKAAA
                     EAVTDALQPGLRTRAYVFNTLLADRRTQDELRGYPSWISSWNLDNEASDESVQALVDA
                     VVARYDIPQRWYRLKAQVLGIDKLADYDRNASVASTKATIGWDRATEIVRDAYASFSP
                     ELADVVGRFIDEGWIDAPTRPGKRGGAFCSYTVPGHHPYVFLNYTATPGDVMTLAHEL
                     GHGLHGYLARPQGVFEQMTPLTLAETASVFGETVTFERLLSMVDDADERFALLAQNLE
                     GNIATVFRQVSMNRFEDAVHTHRREHGELSTEDFADHWARTQGDLFGDAMEVTDGYRS
                     WWSYIPHFIGTPGYVYAYAYGQLLALSVYARYVERGDEFVPKYLELLAAGGSRWPEEL
                     GRIVDCDLADPGFWDAGLAIIDTKLTEAEQAAADAGRL"
     gene            15548..16621
                     /locus_tag="VLR27_10090"
     CDS             15548..16621
                     /locus_tag="VLR27_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSP03841.1"
                     /translation="MELGVCVAAKIDDVDHIVLAEELGYSSAWVADSHLIWSDPYATL
                     AVAATRTERIRLGTGVAVAGIRPEPVTAASIATINRLAPGRTFLGIGSGNTAMRIMGH
                     PPLRIAELDRYLTTLRPLLRGEEAELTWRDRTTRIRHVMPDAGFVDLDHDIPVHVSGF
                     GPRSLGLAGKHGDGVVISGNVTPTGMARIWSAVEAGAEAAGRTIDRETFGATCLTTMV
                     VLDPAEAPDSARSRSLCDAFAIATLHYAYDQWRNFGKAPNQPAVLEVWDDYVAEIERI
                     PEEIRHQHVHRGHNCWVVPEEERFVTRGLIEASCMVGTADELVDRLHELDAAGLDEVL
                     LLPPLATRDLALRLVASDVLPRL"
     gene            complement(16666..17274)
                     /locus_tag="VLR27_10095"
     CDS             complement(16666..17274)
                     /locus_tag="VLR27_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03842.1"
                     /translation="MRSHSDADLELSDDLDGKSRGAVMGLVTLPFRAVGSILGIVVRL
                     VTFLGLIVAVPVRLAVGAVRTVVNLASDLVYALWRAAMTVVRAVLAAFGLLIGAVMAL
                     IGLVRRLIMALFGLLFTLVAAVLKLVKLALTIIAIPIAAVVGIAITIGRVVLKVVTTV
                     LAAVVGILLLPLRLLGIGGKKDKVEVVEVRDASEAKHLPDAA"
     gene            complement(17288..17740)
                     /locus_tag="VLR27_10100"
     CDS             complement(17288..17740)
                     /locus_tag="VLR27_10100"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HSP03843.1"
                     /translation="MSDATDTPGPLPVPEPWVHADAKPFWDATAEGRLCLPRCTDCGT
                     VIWYPRGFCPACSSFDIEWIDAPGTGEVYSFSIPRRGQGAYRDAAPYVVAYVELDLPD
                     GIGGDGPGPRIMTNLVECDPWSVSVGDRVRAVFHPTERGVALVRFRPA"
     gene            complement(17742..>18603)
                     /locus_tag="VLR27_10105"
     CDS             complement(17742..>18603)
                     /locus_tag="VLR27_10105"
                     /inference="COORDINATES: protein motif:HMM:NF006010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HSP03844.1"
                     /translation="VALITLAGKPRTGGSGPGGSGRVGDAPEFSFEVPFGTSVLSNYA
                     LAATRLMHEFGTTSAQLAEIKVAASTHAQHNPNAFLRDVVTVDEVLESPMVCDPLHRL
                     DSCVITDGGGALVVVSPEVARTLDRPCPVVLGHGEAPKHTGNGRIDLTHTGAVWSGPR
                     AFEEAGVTPADIDYASFYDSFTITVLQTIEDLGFCAKGEGGRFVSDGGLQAPHGRLPF
                     NTDGGGLCNNHPTNRGGMTKVIEAVRQLRGEAHPAVQVPDCDIALAHGTGGSLGTRHG
                     SATLILGREA"
BASE COUNT         2523 a         6357 c         7087 g         2636 t
ORIGIN      
        1 caccggcccg aggtcgaagg cgtcggcggc accggtctcg atcagcggca gggcctgcag
       61 gggcttcacc gccgagcggg ggtgggtgag ccgatccggg tcgccccagg accgcacgac
      121 gttgccctcg acgtcggcga ctgcggcgtg gacgacgtgg cggctctcga cgtgcgggcc
      181 gcgccagagc tcgaccgtga tcggcgacgt catgtcggag gacccgagcc gacaggcgag
      241 gggacccatg tcacggtgcc ctgtggcggc ggagccgcca gcccggaccg cgggtggatg
      301 tgaccgatcc gcacagcttt acgagctgca cgagagcatc gagcagcccg cccgctcccg
      361 tgcccactcc ggtcgcttct cggcgaagcg ctcgcgtccg ggctggtcgt cgtaggggcg
      421 gcgcagcgtg tcgtggagct cggcgatgag cgtgtcgtcg tcctgctcgg ccgcgtcgat
      481 ggcgagctgg gccaggtagt tgcgcagcac gtacttgggg ttcaccgcgt ccatcctggc
      541 gcgcggatcg tcgacgtcga ggcgttcgcg ccagcgacgc agccagccgg agaggccggc
      601 gagcacgtcg ccggtgacct cgtcgggggc gtagcaggcg ccgagcaggg gctcgaccag
      661 cgagcggtcg gtggcggccg cgtcgagggt ggcgacgtcg ggcagcgaac ggaagaagat
      721 cgtcatgtcg atctccgtgc tggacagcac ctcgtgcagg tcggcgacca gccggcggtc
      781 gggctcggcc acgacgccgt cgacctcgat gccgagcttg gccagccacg cccggggcag
      841 ctcctgctcg atggtggcgg cgtaggaccc gaggctggcc tcgagcatgg tggcgtcgcc
      901 gacgaggacg tggaaggcgc cgccgaggcg ggacaggttc cactgggcca ccatcggctg
      961 ggtgccgtac cggtagcgct tcatgtactt gtcggtcgtg ttcggggtcc agccctcgtc
     1021 gtagggctcg agccagccgt agggcccgta gtcgatggtg aggccgagga tcgacaggtt
     1081 gtcggtgttc atcaccccgt ggacgaagcc gacccgcatc cagtcgacca tcagcgacgc
     1141 ggtgcgacgg cagacctcgt cgaagaggtc gatcagcagc tcgcggcggt gctggtcgtc
     1201 gcggggcgcg ccgaggtgcg ggaagtcgtg ggcgagggtg tggtcgacga gctggcggag
     1261 gaggtcctcc tcgccgaggg aggccggcag ctcgaaggtg ccgaaccggg tgaacgacgg
     1321 ggccacgcgg cacacgaccg cgcccggctc gggggcgggg tggccgtcgt agaacatgtc
     1381 gcgcacgacc tggtcgccgg tggtgacgag cgacagggcg cgggtggtgg gcacgcccag
     1441 gtggtgcatg gcctcgctgc agaggaactc gcgcaccgac gagcgcagca cggcccggcc
     1501 gtcggccgag cgggagtagg gcgtgggccc ggcacccttg agctgcaggg tctggttgcg
     1561 gccatcggcg tcgacgacct cgccgagggc gatggcgcgt ccgtcgccga gctgaccggc
     1621 ccagttgccg aactggtggc cgccgtagcg gtcggctcgc ggggtcatcc cgtcgacgac
     1681 cgcgttcccg gacagcaccc gggcgatctc gtcggcctcg tccggcgtgg gggaggccgg
     1741 cagcccgagc ggtgcggcga cctccgggga ccaggcccgg agccggggcg cggcgaccgg
     1801 ggtgggccgc acgggggacc acgcggcgcc gggcacctgg cgggggaaga cctcggaccg
     1861 ggcatcgccg gggagggcgc ggacgaagcg gtcgtcccag gtcagcgcgt cgaacagcgg
     1921 ggacggctgg gtggcggcgg tctggagctc ggtgatccgc ccagtctgag gcgacgggtc
     1981 cggtccgcca accctgcgct cgtaacctga cccgcgatgg gtgcaggaca cgaccacggc
     2041 cacggcctcg ccggccagcg ggccggcacc cggcatcgtc gacggctggc gatctccttc
     2101 gggctgatcg ccgtgttctt cgtcgtcgag gcggtggccg gcgtgctcac caactccctc
     2161 gccctgctct ccgacgccgg ccacatgctc accgacgtcg tcggcctcgg catggcgctg
     2221 gccgccatcc agctcgccga ccggcaccag gcgggcgccg cccaccggag cgagaccagc
     2281 cagcacacgt tcggcctcta ccggctcgag atcctcgcag cgttcgtcaa cgccctgctg
     2341 ctggtcggcg tcgccgtcta catcctcgtc gaggcggtgc ggcgcatcgg gggcgacccc
     2401 gagatcgaca ccggcccgat gctggtggtg gcggtgctcg gcctgctcgt caacctggtg
     2461 gcgttcgcgc tgctgcggga gggggccaag gagtcgatca acgtccaggg cgcctacctc
     2521 gaggtcgtgg ccgacaccct cggctcggtc ggggtgatcg tggctgccgt gctcctcgag
     2581 ctgttcggct ggacctgggt cgaccccctg gtcggtgcgc tcatcggcct gtggatcctg
     2641 ccccgaaccg tccgcctcgg ccgccaggcc gtccggatcc tgctgcagtc cgccccgccg
     2701 agcgtggacc tcggggccct gcgcgccgac ctggccgcgc tgcccgacgt gctcgacgtc
     2761 cacgacctgc acgtgtggac gctcacctcc gagatggaga acgccaccgc ccacctcgtg
     2821 gtcgaacccg gcgtcgacgg ccaccacgtg ctcgaccagg cccgggccct cctgcaggag
     2881 cgctatcgca tcgcccacgc cacgctgcag gtcgagccgt ccgaccacac ggggtgcgac
     2941 gagctcgagt ggtgaccgcc gcggcccggc gtgccgtggc cgctgccaga agtcgcgggg
     3001 gtcaggactc ggtggggccg gtgaagtcgg ggtcgcgctt ctcggcgtgg gaggccaggc
     3061 cctcccgggc gtcggggccg gcgaagccga ggaactcgta ggcgagcgac gcatcgaagg
     3121 tggccgactg ggcccggaac cagtggttga gggtgtgctt ggtccagcgg atggccgact
     3181 gcgcgccgtt cgccagcttc accgccacct ccatggcccg gtcctggacc tcttcgtcct
     3241 cgacgcacag cgagacgagc ccgatgcgct cggcctcctc gccggtgagg gtgtcgcagg
     3301 tgagcaggta gtacttggcc ttggccatgc cggccatgag cggccagcag atggcggcgt
     3361 ggtcgccggc ggcgacgccg aggcgggtgt ggccgtcgat gatgcgggcg gtgcggccga
     3421 ccacggagat gtcggcgagg atgccggcca ccagcccggc gccgacggcc gggccgtgca
     3481 tggccgacac gatcggcttg ctgcacccga tcacgttgaa gaccaggtcc cgggcctcgc
     3541 gcatggcccg caggcggaac tcggggtcgt ccatgatcgt ctcgatcatg tcgaagctgc
     3601 cgccggagct gaaccccttg ccggcgccgc gcagcagggc gacgcgggtc tcggggtccc
     3661 ggtccacggc cagccacacg tcggcgagct ggcggtgcat ggcggcgctg accgagttga
     3721 gcccgggtgc atcgagggtg atccgcagca ccccgtcggc gggtcggtcg aaggtcagct
     3781 cgtcgaagtc ggcgtaggga tcagtcgtcg tcgtcatcgt ccccatcatc gccgccccga
     3841 ccgttccccc ggtccttctt gtccggcttc tcgtccttgt cgtccttgtc gtcgtcgtcg
     3901 tcatcgtcga agacgggagc cggcggggtg gtcggtggcg ccgtggtggt ggtcgactcg
     3961 gtggtggtcg tgggctcggg gagctcctgg tcgacctggg cccgggcgtg ctcgatcaac
     4021 cgggcgagct cggcgacctc ctcgtcggtg atctcgcctt cggcgtgggc gtagaagccg
     4081 tcggtgccga gctcctccag cccggccagc gcggccgcac gatcgccggc ctcgacggcc
     4141 tggcggacct ggtcgacctg cgcgtcgtag cggccggtgt cttcgtcgcc gcacgcgacc
     4201 agcaggaggg cggcggccac ggccgccacc agtcggcgga tcaccggccg agctcctcga
     4261 tgcgatcgag ggtgccgtcg agcgaggcgg gagcctcctg cccggcgtcg gcggtgcccg
     4321 ggtccgggtc ggggtcggtg tcgagggcga cgacgagcag cagcaggagg atcaggagcg
     4381 cggcgacgag gccgatgatg acctcgggac gccggccgtg ccggtcgagg aagctccgca
     4441 cgccctcgag gggcccgggg ccgtcgtcgt ggggctgcgg tgccggtgcc ggtgctggtg
     4501 ctgggtccgg cgtcggtcgg gcggggacgg tgacggtgcg ctcccgtgcc ggatcgggag
     4561 cgacgtcgtc ggccgccggg acgaccacgg tggcctcgga cgggtcgggc tcggtcaccg
     4621 agcgcggcag cacggaggtg gcatcgaggc ggacggtggg atcgacgggg aggggggcac
     4681 cggagcggat ggcggcgagc atcgactgcg cgtcctcggg acggtcgtcg gggtcgggcg
     4741 acagcgccag gccgaggacc tggcggaggc gctcggggac cgtctcgggc agcggcggtg
     4801 cgtcgccggt gcggagcgcg gcgatggact ccacggcctg ctcgcgccgg aacggctgga
     4861 tgccggtggc ggcctcgtgg atgaccacgg cagcggagaa gacgtcggac cgggtggagc
     4921 cccgggcacc gtcgaggcgc tccggtgcca cgtacaccag cgtgcccatg atgtcggtct
     4981 cggtgctctg gtcgtcgtcg aggctggcca ggccgaagtc ggtgacgcgg aagccaccgt
     5041 cgttggcgag gacgttggcc ggcttgacgt cgcggtggag cacgccggcg aggtgggcgg
     5101 cgccgagggc gccgagcagg tcgtcgacca gctgctccag gcgctccggg gggaggggac
     5161 cgtcgcggag ctcgtcggcg agggtgtgcc cgtcgtggca ctcgagcacc atgtaggggt
     5221 tgccgtcggc ggtgcgggcg aggtcgagca cggtgacgac ccgggggtcg gtgatccggg
     5281 cggcagcccg cgcctcgcgc tcgagctggg ccgcggccga ggggggcagg tcgtcgcgca
     5341 ggaccttcag ggcgaccgtg cggccgaggc ggatgtcgta ggcctcccac acggtgccca
     5401 tgcccccgga gccgagcacg gagcgcagct cgtagcggtc ggcgacggtc ggctcggcca
     5461 ggtccgtgcg cacgaggggc acggcaggga agtcggctct gccggcggtc acctggggga
     5521 gataccccgg gcccgggcgg aacagaccac cgatggcaga ctcacccgcc gtggcagacc
     5581 ggcagaccat cgtgaccctc gatctcgagg gggtgctcgt gcccgagatc tgggtggcgg
     5641 tcgccgaccg caccggaatc gatgccctgc gccgcaccac ccgggacgag ccggactacg
     5701 acgtgctcat gcgctaccgg ctcgacgtcc tcgacgagca cgggctcacc atgtcgctgg
     5761 tgcaggacgt catcgccggc ctcgagccgc tgcccggcgc gaaggcgttc ctggacgagc
     5821 tccgcgagcg cacgcaggtc gtgatcctgt ccgacacctt cgagcagttc gccgcgccgc
     5881 tcatgcgcca gctcggcatg cccaccgtgt tctgccaccg actcgtggtg gaggacgacc
     5941 gcatcgtcga ctaccgcctc cgccagcccg accagaagca gtgctcggtg aaggccttcc
     6001 agtcgctcga gtacaaggtg atcgcggcgg gcgactccta caacgacacc acgatgctcg
     6061 gcgcggccga cgccggcttc ctgttccggg ccccggagaa cgtcatcgcc gagttccccc
     6121 agttccgcgc catgaccggc tacgacgagc tgctcgacgc cctcacggcc gagctgtagg
     6181 ccgaccggcc acgggtcggg ccggggccgg gcagcagccc gggcggcacg ggttcacccg
     6241 agggccgagc gcgccgagcg ccgccggcaa ccgcccgacc aggtgctcct cgagcacgtc
     6301 gaccaggccg cgcacgaaca gcgggtgggt gcccaccgtg ccggcgcgca ccatcgtcat
     6361 gcccagctcc ttcgcggcgt cggcggcctg ggtgtcgagg tcgtagacca cctccatgtg
     6421 gtccgacgtg aacccgatcg gcaccacgat caccacgtcc tcgccccgtc cgtgcagggc
     6481 accgaggtgg tcgacgatgt cgggttcgag ccacggcacc tgcgggggtc cggaacggga
     6541 ctggtagacg aggtcccagc cccggtcgtc gtcggcgccg ccgttccgtg cggcgccgat
     6601 ccggggcacc gccacccgct ccacgatcat cgtcgcctcg tcgagcagct gggcctggta
     6661 gtccgaggtc tcggccatcg acagcggcac cgagtgggcg gtgaagacga gccgggcccg
     6721 gttgcggtca gcggcggaca gcgcctcgag ggcggcgtgc acccgctcca ccatcgccag
     6781 cacgaagccg gggtggttcc agtacgggcg gaccttctcc accgccggcg gatcgcctcc
     6841 ggcatcgcgg accgcggcca cggcccggtc gatgtcgtcc aggtactgcc ggcacgcgga
     6901 gtacgaggag taggccgagg tcgagacggc cagcacccga cggtggccgg cggcggtgat
     6961 ctcgccgagc acgtcctcga cgaacggcac ccagttgcgg ttgccccagt gcagcgggag
     7021 gttctggccc cgccggtcga gctcggcccg cagcgcctcg agcagcgcct tgttctggtc
     7081 gttgatcggg ctgcggccgc cgaacagcag gtactgctcg gccacctcct ccaagcgctc
     7141 ccggggcacc cctcgcccgc gggtgacgtt ctcgaggaag gggacgacgt cgtcggggcc
     7201 ctcgggcccg ccgaagctga gcagcaggat cgcatcgaag gccggcgtca tcggacgcgt
     7261 gtctacccgg tggagcgggc ggttgggccc gcgaggtcgt ccccggtcag gctggctcga
     7321 tggagctgct gctgatccgc cacgcgctgc ccatccgggt cgagggcgcg gcgggacccg
     7381 ccgatccgcc cctgtcggac atcggccgcc gccagtccga gtcgatggtc cgctggctcg
     7441 agcccgagcc gctcgatgcg ctcgtcgtca gcccgatgat ccgggcccgg gagaccgcca
     7501 ccccgctcgc cacggcgcgg gggttggaga cggtggtcca cgagggcgtc gccgagttcg
     7561 acaaggacgc cgactcctac gtcccgatgg aggagctgaa ggcagccggg gaccctcgct
     7621 ggcaggagat cgtcgacggc ggctacttcg gcgacggcga cctcaccccc gagcagttcc
     7681 aggcgaccgt cgtcgacgcg gtcgaggagg tcatcgccgc caatgccggc ggcaccgctg
     7741 ccatcgtgtg ccacggcggg gtcatcaacg cgtacgtcgc ccacgtgctc ggcatcgacg
     7801 acctgctctt cttcgagccc ggctacacga gcatcaaccg gatccgggcc tcccgacggg
     7861 gccaccgcat ggtcgtcagc ctgaacgaga cggcccacct ccgcgacgtg tagatcctga
     7921 cccgcgggtc aagtccgcgc ctacggtgtg gcgaccgcac gatcaggggg atgcacatga
     7981 aggtcgacgc cgacgctgct ggactggacg cgacgcgcct gggacggatc gccgagcacc
     8041 tggagcagcg ctacctggag ccgggcaaga tcgccggcgc gcaggtggcc gtgctgcgcc
     8101 acggggagct cggctacttc gagtccttcg gccagcggga tcgcgagcgg gaccagcccg
     8161 tcaccgagga cacgatctgg cgctggtact cgatgaccaa gccgatcacc ggcgtggcgc
     8221 tgatgaccct ctacgagcgg gggcacttcc agctcgacga tcccgtccac cgctggatcc
     8281 ccgagtggcg tgacatgcag gtgcgcgagt ccgaccccga cgggggatcc cggatcgtgc
     8341 cggcgcaccg gccgccgacc gtgcgcgacg tgctcaccca cacctccggc atcgggtacg
     8401 gcccggagaa ccgggacctc gacctcgtga ccgaccgcgg cttcctcggc gggcacgacc
     8461 tcgagtccct cggccaggcg atcggcggat ggccgctgcg cttccagccc ggcaccaagt
     8521 ggctgtactc cttcggcatg gacgtggccg cccgcctggt cgaggtcatg tccggcctgc
     8581 gcttcgacga gtacctgcgc accgagctgt tcgagccgct cggcatggtc gacaccggct
     8641 tctccgtgcc ggaggacgac ctcggtcgct tcgccgcctg ctacggccgc aacagccgca
     8701 aggagctggt gctggtcgac gaccccgagc gcagctcgta ccggcgcgag cccaagctgc
     8761 tcaacggcgg cggtgggctc gtcggcacca ccggtgacta cctgcggttc gtggccatgc
     8821 tcgccaacgg cggcgagctg gacggccggc gggtgatctc ccgcaagacc ctcgagctca
     8881 tgggcagcaa ccacctgccc ggcgacggcg acatggcgga cttcgccctg ccgatggggt
     8941 acggggaggt cgggttctcg ggtgcggggt tcgggttgac catggcggtc tccaagggcc
     9001 cggcggccac cggcgtggtg ggttcggcgg gcgagatgat gtggggcggg gcggcctcga
     9061 ccacgttctg ggtcgatcgg gccgaggagc tggcggtggt gttcatgacc cagctgctcc
     9121 cttcggggac gttcgacttc cggaaccagc tcaaggcctt ggtgtacggc gccatcgcgg
     9181 actgaacggt cgagaggccg gggcgtggtg ccccggcctc ccgtcccctg tgcggtgtcg
     9241 ccgctcggcg gcgggatggc tcagctgacg gtcagctcgg cgaacatgcc ctggctgacg
     9301 tggggcgggg cgtcgctctc cggcgggccg tcggcctccg gatcgaggat ggagtcgtcg
     9361 gcgccgacgg gcaggaagca gacgatggcg tagcgaccgg gctcggtcag cgtgccgtcg
     9421 ccgacgaccg ggccgggcgt gtcggtggtg ccggtggcgg cgaggatcac cgcggcgggc
     9481 tcaccgtcgc cgaagatggg gaagagctcg tcctcgggca gcgccatcag ctccgagatc
     9541 gggcgggtct cctcgtcggg gatgcgcatg gcgaccagct cgtggggctc ctcaccctcg
     9601 ttggtgagcg acagcttcgt gccggcctcg accgacgccg gcaggccctc gaaggcccag
     9661 tcgacggcgg tgacctcgac gacctcgcct tcaggggcgt cgtcggactc gtcgccggtc
     9721 tcggtctccg tctcgttgtc gacggcttcg gtggtggggg tctcgtcggc cgcggcgtcg
     9781 tcgtcgtcac cgcacgcggc gaggccgagg gccaggaggc acgcggcgac cgcggcggcc
     9841 cgacggcgga tggtggtggt gttgttcttg ggcatgtggt caccgtgccg ccgacggctt
     9901 ggagggggcg tggagaccgc tggaagcccg ggttcagggg tgcggcggag cgagtcgggg
     9961 tgcgaggtcg tgcacgagcg catcgatctc ccgggcgcag gccgcgaaga cgtcggcctc
    10021 gcaaccgccg gggtcgacga cctcctccca cggctccagc tccacctcat ggggggccag
    10081 tgcggcgacc tggtcggtga gggtcggcgc cggcaccagg tgctccacca ggcgacgcag
    10141 cgtgaccgtg cggcgagcgg caggcgcgtg ggtgcgccgg acccactcga cgtgctcggg
    10201 cgccagggcc atcaccaggg tggcggcgtc gaggtccggc gggtgcacct ggcggctggc
    10261 gtggcggggg atctcgaggc ccaccccctc gatggcggcc cgggtgcgga tgctcatggg
    10321 ctggccgtcg accacgaagg tcccgcccgt cgcgacccgc aggtcgggtc gtcgcgccga
    10381 cagcgacgtg gccgccatca ccgagcgcgc cgcgttcccg gtgcagagga tgaggacgtc
    10441 gaccacggca ggagtctgcc caccgctcgc ggcccgctcc cagccggtgc ctgtgcgaac
    10501 ctgagcgcca tgacgcagct gctgcccctg accgccgacg aggtcctcgc cacgacgcga
    10561 gccgtccgca agcgcctcga cttcgaccgc cccgtggagg acgaggtgct gcgggagtgc
    10621 ctcgagctgg cgctgcaggc accgtcgggc tcgaacgccc agtcgtggcg gttcgtcgtg
    10681 gtcaccgacc cggacaagaa gcaggccctc ggcgagctgt accgccaggc gttcgacatc
    10741 tacgagcagc tcgacggcat caacgccgcc accatctacc ggggtgacga ccccgagcgg
    10801 ctggagcagc agcagcgggt gatgggctcg gcccgctacc tcgccgagcg gatgggcgag
    10861 gtcccggcga tgttggtgcc ctgcatgccg ggccgcagcg acggtgcgcc tgccgtgtcg
    10921 gcggcggcgg cctacggatc gatcctgccg gcggtgtgga gcttcatgct ggccggccgc
    10981 gctcggggcc tcggcacgtc gtggaccacg ctgcacctga tgttcgagca gcaggcggcc
    11041 gagatcctcg gcatcccctt cgaggagtgg acccaggtcg cgctggtcag cgtcggctac
    11101 acgaagggcc ccgacttcaa gcgggccacg cgggagccgc tggagaccgc cgtgtcgttc
    11161 aacggctggg acttcgagga ctgagcgaac cgaccccgga cgcgacgaga gcgccgaccc
    11221 cgaggggccg gcgctctcga tcgacgtgcg ggttgctctc ttcggagccg agccgtcagg
    11281 cgaggcgacc catgtcacgg tgcgctacgg cggcggagcc gccatctcga ccggcatcgg
    11341 gcctcacccg tcggtgcggg tcctacagag cgcgaacgtt gtgggcttcg tcgcccttgc
    11401 ggcccggtcc gatgtcgaac tcgacctgct gaccttcgtc gagggacttg aagccctcgc
    11461 cggcgatgtt ggagaagtgg acgaacacgt cgtcgccgtc ctcgcgggag atgaagccga
    11521 agcccttctc agcgttgaag aacttcacag tgcctgtagc caaaacaatc ttctttcaat
    11581 aacgataaac cgacccgtcg gatttgacgg gccggcagcg accacggtac ccgcgaccag
    11641 cgctgggcgc accctcggag tgcgatcggg gtcagtcggc gtggtggagg gtcgccgtcg
    11701 tggtcaccga ggcgagcacg tcgaggtcga cctcgacccc gatccccggc ccctgcggca
    11761 cccggatcgt gccgtccggc tcgagcacgg cgggctgggt gacgacgtcg gtctcccagt
    11821 agcgggcggc ggccgagatg tcgccgggga gggtgaagcc gggcagggcg gccagcgcca
    11881 ggttgggggc ccggccgatg ccggtctcca gcatcccgcc gtgccagacc ggcaccccgg
    11941 ccgcgaggca ggcatcgtgg atgcgcacgc cggcgaggtg accgccgacg cgaccgggct
    12001 tgatgttgat gacgtccgct gcgccgaggc ggagcacgtc gtcgcacacg gcttcgtcga
    12061 cgatggtctc gtccaggcag accggggtct gcagcgcctg aaccaggtcg acgttgtcga
    12121 cgatgcgctc ctcggggtac ggctgctcga tcagcagcag gccgaactcg tccagggcgc
    12181 gcagcgtgtc gacgtcgccg gggccgtagg ccgcgttggc gtccacctgc agcccgaagt
    12241 cgtcgccgag ctcggcccgc agccgacgac acggctcgac gtcccagccc ggcttgatct
    12301 tcaccttgat gcgggcgtag ccctcgtcga ggtggcgggc ggcggtggcg accaggtcgt
    12361 cgatggtggg cacgatgccg atggacaccc cgacgggcac gcggtcaacc gcggcgccga
    12421 ggtgggcggc catgctgcgc ccggcggccc gcagctcggc gtcgagcaca gcggcctcga
    12481 ccgcagcctt ggccatgcgg tggccccgca cctcctggag ggccccggcg acgtcgtcgg
    12541 cgtggcggac cccggccgcg aacaggcggg ggagcaggtg atcgaccgtc acgtcctcgg
    12601 cggccgaggt gtactcgctc gagtagagcg gctgggtgcc ggccacgcac tcgccccagc
    12661 cctcgaccgg gccgtccggt ccctcggcca gcacccgcac gaggaggatg tcgcggaacg
    12721 tccacgtgcc gaagctggtc tggaacgggg tgaccagggg cagctcgatg cggcgcagct
    12781 cgaccgatcc gatgtcgatc ccggggagcg ctcctcccga actggcagtc gtggtgttgc
    12841 ggtcagcggc cgaacgggtg gggtcgggtc cggtcatcgg tcctccggtc gggtgaacag
    12901 gtagcggcgg tcggtggtga cggccacggg ctcgagaccc tcggcgaagg cctgcagcat
    12961 cgtctcacgc accgcaaggc gccacgcccg ggccatctcg gggtcgtcgc ggcggagggc
    13021 gaccgcgtcc gcgggtgtcg cgatcagtcg gaccgcggcg tcggtgtcga ctcggcgggg
    13081 gcggcccccg tcgtcggcca gggcaacatc ggcatgggcc acatccgggg tcggcagcgg
    13141 gcgatcgagg gcgatgcggg tgcgctcgtc gtccaggtcc cagcggatga gcagccggtc
    13201 ggaggcgtcg ccccggttga tctcgtcctc catgtgcccg tacagctcca cgaggtagcg
    13261 ctcgccccgg gcgccgaggt ggtggaggtt gaaccgggcg ttggcatgga tcaggggatc
    13321 gaaggtccag ctgatggtgg tgatgccgcg ctcgaggcac cactcgcgct ggtgccgctt
    13381 cagggcccgg ccgatgccgg cgccccggtg ggcggggtcc acggccgcga ggtgcgagtg
    13441 caggtggagg tggtcgtcgt gcacgccgac gatgccgagg cacatgccga tctggcggcc
    13501 cgacccgtcg agcgcgaccg agacgtagct gccggccagc tccagggccc gcagcaccgc
    13561 gtcgttcagg gggcgctggc cccacaccga ctcggccagg tcgagggcgc ggtcggcctc
    13621 cgcacccgtc accgtccgga tctcgtactg ggccgcggca gccgacatct gatgcgtgtc
    13681 ccgcgtcccg gaacggatca caggcggccg gcgtcggcgg cggcctgctc ggcttcggtg
    13741 agcttggtgt cgatgatggc gaggccggcg tcccagaagc cgggatcggc caggtcgcag
    13801 tcgacgatgc ggcccagctc ctcgggccag cgggagccgc cggcggccag cagctcgagg
    13861 tacttgggga cgaactcgtc gcctcgctcg acgtagcgcg cgtacaccga cagggcgagc
    13921 agctgtccgt acgcgtaggc gtagacgtag cccggggtgc cgatgaagtg cgggatgtag
    13981 ctccaccagc tgcggtaccc gtcggtgacc tccatggcgt cgccgaacag gtcgccctgg
    14041 gtgcgggccc agtggtcggc gaagtcctcg gtggacagct cgccgtgctc gcgccggtgg
    14101 gtgtggacgg cgtcctcgaa ccggttcatc gacacctgcc ggaacacggt ggcgatgttg
    14161 ccctcgaggt tctgggccag cagggcgaag cgctcgtcgg cgtcgtcgac catggacagc
    14221 aggcgctcga aggtgacggt ctcgccgaag acgctggcgg tctcggcgag cgtgagcggg
    14281 gtcatctgct cgaacacgcc ctgggggcgg gccaggtagc cgtgcaggcc gtggccgagc
    14341 tcgtgggcca gcgtcatgac gtcgcccggc gtggccgtgt agttcaggaa gacgtagggg
    14401 tggtggccgg ggacggtgta ggagcagaac gcaccgcccc gcttgccggg tcgggtgggg
    14461 gcgtcgatcc agccctcgtc gatgaagcga ccgaccacgt cggcaagctc gggcgagaac
    14521 gaggcgtacg cgtcgcgcac gatctcggtg gcccggtccc acccgatggt ggccttggtg
    14581 ctggccacgc tggcgttgcg gtcgtagtcg gcgagcttgt cgatgccgag gacctgcgcc
    14641 ttgagccggt accagcgctg ggggatgtcg tagcgggcga cgaccgcgtc gacgagcgcc
    14701 tgcaccgact cgtcgctggc ctcgttgtcg aggttccagg agctgatcca gctcgggtag
    14761 ccccgcagct cgtcctgggt gcggcggtcg gccagcaggg tgttgaacac gtaggcgcgg
    14821 gtgcgcaggc cgggctgcag cgcgtcggtc acggcctcgg cggcggcctt tcgggtggcg
    14881 cggtcggggt cgagcaggcg gctgaggccg gcgtcgaggg gcaccggctc gggctcgtcc
    14941 ggaaggtcga cctggatcga cgaggtctgc tcggtgaaca gccggaccca ggcctggcga
    15001 ccggtcggtg ccgtgttcgc gagcaggcgc tcctcgacct cggagaggac gtggggcttg
    15061 ccggcccgca gggtctcgag gtggtggcgg tgctcggcca gcgcgggatc gtcgagcagc
    15121 tgcgcggccc ggtcgtcgtc gatggccacg acctcgaggt cgaaccagac cagctcggcg
    15181 gacagggcgg ccgaccgctc gctgacctcc tgcatcagcg cgccccgggc tgggtcggag
    15241 gtgtcgacgg cgaagtcgag gctggcgtag ctgccggccc ggcgggcctg ctcgtagatc
    15301 tcggccaacc ggtcgagggc ggcgctgagg gtggatgcgt cggcgtcggt gagcgtgccc
    15361 ttcacgtcgg cggtgagcgc gcgagccgac tcgatcgcct cgtcgagcag gcggcggacg
    15421 ccgtcggcgc cctcaccatc gacgagggtg ccgatgtccc acgccacccc ttccgcgccg
    15481 gtgacctcgg gtgcgtgcgt ctcggtcgac atcttgccga gcataggaac gcctacggtc
    15541 cggtgccatg gagctcgggg tgtgcgtggc ggccaagatc gatgatgtcg accacatcgt
    15601 gctggccgag gagctcggct actcgtcggc gtgggtggcc gacagccacc tgatctggag
    15661 cgacccctac gccacgctcg ccgtcgccgc gacccgcacc gagcgcatcc ggctcggcac
    15721 cggggtcgcc gtggccggca tccggcccga gccggtgacc gcagcgagca tcgccaccat
    15781 caaccgcctg gcgcctggtc gcacgttcct cggcatcggc tcgggcaaca cggcgatgcg
    15841 gatcatgggg cacccgcccc tgcgcatcgc cgagctcgac cgctacctga ccacgctgcg
    15901 gccgctcctg cgaggggagg aagccgagct gacgtggcgg gaccgcacga cgcgcatccg
    15961 ccacgtgatg ccggacgccg gcttcgtcga cctcgaccac gacatccccg tccacgtctc
    16021 cggcttcggt ccccgctcgc tcggcctcgc cggcaagcac ggcgacgggg tcgtcatctc
    16081 cggcaacgtc acgccgaccg gcatggcccg catctggtcc gccgtggaag cgggcgccga
    16141 ggctgcgggt cgcacgatcg accgcgagac gttcggcgcc acatgcctca cgaccatggt
    16201 ggtgctggac ccggccgagg cgccggactc ggcccgctcc cggtcgctct gcgacgcgtt
    16261 cgccatcgcc acgctccact acgcctacga ccagtggcgc aacttcggga aggcgcccaa
    16321 ccagccggcc gtgctcgagg tgtgggacga ctacgtggcc gagatcgagc ggatccccga
    16381 ggagatccgc caccagcacg tccaccgtgg ccacaactgt tgggtggtcc ccgaggagga
    16441 gcgcttcgtg acccgcggcc tcatcgaggc gtcgtgcatg gtcgggaccg ccgacgagct
    16501 cgtcgaccgg ctgcacgagc tggacgccgc cggcttggac gaggtgctgc tgctgccgcc
    16561 gctggcgacg cgggacctgg cgctgcgcct cgtcgcctcg gacgtgttgc cgcggctctg
    16621 agtcggtttg ctggcggcca gttcgggtca ccgccttcgc ttcgctcagg cggcgtcggg
    16681 gaggtgcttg gcctcgctgg cgtcgcggac ctcgacgacc tcgaccttgt ccttcttgcc
    16741 gccgatgccg aggaggcgga ggggcagcag gaggatgccg acgacggcgg cgagcacggt
    16801 ggtgacgacc ttgagcacga cgcggccgat ggtgatggcg atgccgacca cggcggcgat
    16861 ggggatggcg atgatcgtca gggcgagctt caccagcttg aggacggcgg ccaccagcgt
    16921 gaacagcagt ccgaagaggg ccatgatcag ccggcgcacc agaccgatga gcgccatgac
    16981 ggcgccgatc agcaggccga aggcggccag cacggcgcgc acgacggtca tggcggcgcg
    17041 ccacagcgcg tagacgaggt cggaggcgag gttgacgacc gtgcgcacgg cgccgacggc
    17101 gagccggacc gggacggcga cgatcaggcc gaggaaggtg accaggcgga cgacgatgcc
    17161 gaggatgctg cccacggctc ggaaggggag ggtgaccagc cccatgaccg cccctcggct
    17221 cttgccgtcg aggtcgtcgg agagttcgag gtcggcgtcg gagtgggagc gcatgggtcc
    17281 gatcttgtca ggctgggcgg aagcggacga gggccacgcc gcgttcggtc gggtggaaca
    17341 ccgcccttac ccgatcaccc accgagacgg accacggatc gcactcgacc aggttggtca
    17401 tgatccgggg gccgggaccg tcgccgccga tgccgtcggg caggtcgagc tcgacgtagg
    17461 ccaccacgta cggggcggcg tcgcggtagg caccctgccc ccgccggggg atcgagaagc
    17521 tgtacacctc gccggtgccg ggggcgtcga tccactcgat gtcgaacgac gagcacgccg
    17581 ggcagaaccc ccgcgggtac cagatgacgg tgccgcagtc ggtgcagcgg ggcaggcaca
    17641 ggcgcccctc ggcggtggca tcccagaacg gcttggcgtc ggcgtggacc cagggctcgg
    17701 gcacggggag cgggccgggg gtgtcggttg cgtcgctcat ctcaggcctc ccgtccgagg
    17761 atcagcgtgg ccgagccgtg gcgggtgccg agcgagccgc cggtgccgtg ggccagggcg
    17821 atgtcgcagt cgggcacctg caccgccggg tgggcctcac cacgcagctg gcggaccgcc
    17881 tcgatcacct tggtcatccc gccgcggttg gtcgggtggt tgttgcacag accgccgccg
    17941 tcggtgttga acggcagccg accgtgcggt gcctgcagcc cgccgtcgga cacgaagcgg
    18001 ccgccctcgc ccttggcgca gaagccgagg tcctcgatcg tctgcaggac cgtgatcgtg
    18061 aagctgtcgt agaacgaggc gtagtcgatg tcggcgggcg tgaccccggc ctcctcgaag
    18121 gcccgcggcc cggaccagac cgcaccggtg tgggtcaggt cgatgcgccc gttaccggtg
    18181 tgcttggggg cctcgccgtg gccgagcacc acgggacagg ggcggtccag ggtgcgggcc
    18241 acctcggggg agacgacgac gagcgccccg ccgccgtcgg tgatgacgca gctgtcgagg
    18301 cggtgcagcg ggtcgcacac catcggcgac tccagcacct cgtccaccgt gaccacgtcg
    18361 cgcaggaagg cgttcgggtt gtgctgggcg tgggtgctgg ccgccacctt gatctcggcc
    18421 agctgcgccg aggtcgtgcc gaactcgtgc atgagccggg tagcggcgag ggcgtagttc
    18481 gacagcaccg acgtgccgaa ggggacctcg aaggagaact ccggggcgtc gccgacgcgc
    18541 ccgctgccgc cggggccgga gccgcccgtg cggggcttgc cggccagggt gatcagcgcg
    18601 acc
//
LOCUS       DATISU010000252         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_619478, whole genome shotgun sequence.
ACCESSION   DATISU010000252 DATISU010000000
VERSION     DATISU010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619478"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VLR27_10110"
     CDS             <1..232
                     /locus_tag="VLR27_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSP03845.1"
                     /translation="QEQVLDEAIALAERIAANAPLSIAASKQMIRNSAGRTEEEGWAE
                     QGPLAGKIFSSKDSIEGSIAFAEKRQPNWSGE"
     gene            complement(293..1168)
                     /locus_tag="VLR27_10115"
     CDS             complement(293..1168)
                     /locus_tag="VLR27_10115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSP03846.1"
                     /translation="MTAIIVNYNSAPDIFECLDSLRASEHPVELVVVDNASTDGSPDE
                     LAVLDDVRLIRSARNVGFGGGVNLGVRTVDPDGAILLVNPDATLAPDAVSHLVATLEA
                     RPLAAAVGPLVANPDGTVQPSKRIFPSWTQAAMHSTIGVFWPGNPGTRAYVCADAPED
                     RPSLVGWLSASVLLIRVDAFRDVGMFDERFFFYVEDLDLCRRFADAGKELWFEPRAEA
                     VHAWGGSTRKPTKQLWQHHTNLFRYVRKHSHGWRSAKLPIVALGLSARFALLFARMKL
                     LGRRLPAHSGRARKG"
     gene            complement(1165..2244)
                     /locus_tag="VLR27_10120"
     CDS             complement(1165..2244)
                     /locus_tag="VLR27_10120"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HSP03847.1"
                     /translation="MRVLLDARPLPGHRLGVAQYVLRLAEHAARQVDLEVLAKASDEA
                     DFPPPVHVSRDLPRPLRLAVELALDGRRVNRLAPDVFHGVHYTLPRGLRVPATVTFHD
                     ATMLTMPEVHETSKRLFFQRAIPAGIARADQVICVSESARQGAIAHAGADPARTHTVP
                     LAVDHDRYRPADGDRPAEVRSICDGPYLLWVGALEPRKDVPTLIRAFEALAGEIPHRL
                     VLVGPDAWGAEVITERIAASPVRDRIVRTGWISEDTKADLYRHADAFAYPSLAEGFGL
                     PVLEAMACGTPTVTTTGSAPEEVAGDAAITVAPGDAEALAGAIGRALGEDAARLRAAG
                     PARAARYTWEDTASRTVEVWRRACA"
BASE COUNT          330 a          826 c          980 g          305 t
ORIGIN      
        1 gcaggagcag gtgctcgacg aggccatcgc cctggccgag cgcatcgccg ccaacgcgcc
       61 gctgtccatc gccgcctcca agcagatgat ccgcaactcc gccggtcgca ccgaggagga
      121 gggctgggcc gagcagggcc ccctcgccgg caagatcttc tcctcgaagg actccatcga
      181 gggttcgatc gccttcgccg agaagcgcca gccgaactgg tccggcgagt agcaccgccg
      241 ccgctgttcc gtggtcgctg ccagaacgat ctggcagcga ccacggaacg catcagccct
      301 tgcgggcgcg gccggagtgc gcgggcaggc gccggcccag cagcttcatg cgggcgaaca
      361 gcagcgcgaa gcgggccgac aggccgagcg ccacgatcgg cagcttcgcc gaccgccagc
      421 cgtgggagtg cttgcggacg tagcggaaca ggttggtgtg gtgctgccac agctgcttgg
      481 tgggcttgcg ggtgctgccg ccccaggcgt gcacggcctc ggcccggggc tcgaaccaca
      541 gctccttgcc ggcgtcggcg aagcggcggc acaggtcgag gtcctcgacg tagaagaaga
      601 agcgctcgtc gaacatgccg acgtcccgga aggcgtcgac ccggatgagc agcaccgagg
      661 ccgacagcca gccgaccagg ctggggcggt cctcgggggc gtcggcgcac acgtaggcgc
      721 gcgtgccggg gttcccgggc cagaacaccc cgatggtcga gtgcatggcc gcctgggtcc
      781 agctcgggaa gatccgcttg gagggctgca ccgtcccgtc ggggttggcg acgagcgggc
      841 cgacggcggc ggcgagcggc cgggcctcga gggtggcgac caggtgggac accgcgtcgg
      901 gcgccagggt ggcatcgggg ttcacgagga ggatggcccc atcggggtcg acggtgcgca
      961 cgccgaggtt cacgccgccg ccgaagccga cgttgcgggc ggaccggatg agccgcacgt
     1021 catcgagcac ggccagctcg tcggggctgc cgtcggtcga tgcgttgtcg acgaccacca
     1081 gctcgaccgg gtgctcgctc gcccgcagcg agtcgaggca ctcgaagatg tcgggtgcgg
     1141 agttgtagtt gacgatgatc gcggtcacgc gcacgcgcgc ctccagacct cgacggtgcg
     1201 gctggcggtg tcctcccagg tgtagcgggc ggcgcgggcc ggacccgcag cgcgcaggcg
     1261 ggcggcgtcc tcgccgaggg cgcgcccgat ggccccggcc agggcttcgg cgtcgccggg
     1321 ggccaccgtg atggcggcgt cgcccgccac ctcctcgggg gcggagccgg tggtcgtgac
     1381 ggtcggcgtg ccgcaggcca tggcctccag caccggcagg ccgaagccct cggccagcga
     1441 cgggtaggcg aaggcgtcgg cgtggcggta gaggtcggcc ttggtgtcct cgctgatcca
     1501 cccggtgcgc acgatgcggt cccgcacggg tgaggcagcg atgcgctcgg tgatgacctc
     1561 ggccccccag gcgtcgggcc cgaccagcac cagtcggtgg gggatctcgc cggccagcgc
     1621 ttcgaaggcc cggatgagcg tggggacgtc cttgcggggc tcgagggcac ccacccacag
     1681 caggtagggc ccgtcgcaga tcgagcggac ctccgccggg cggtcgccgt cggccggccg
     1741 gtagcggtcg tggtcgacgg cgagggggac ggtgtgggtg cgggccgggt cggcgccggc
     1801 gtgcgcgatg gcgccctgac gggccgactc ggagacgcag atgacctggt cggcccgggc
     1861 gatgccggcg gggatggccc gctggaagaa cagccgcttg ctggtctcgt ggacctcggg
     1921 catggtcagc atcgtggcgt cgtggaacgt caccgtggcc gggacccgca ggccgcgggg
     1981 cagcgtgtag tgcacgccgt ggaagacgtc gggggcgagg cggttcaccc ggcggccgtc
     2041 cagggccagc tccaccgcca ggcgcagcgg gcgggggagg tcgcgcgaca cgtgcacggg
     2101 gggcgggaag tcggcctcgt cgctggcctt ggccagcacc tcgaggtcga cctggcgggc
     2161 ggcgtgctcg gcgaggcgca gcacgtactg ggccacgccc aggcggtggc ccggcagcgg
     2221 ccgggcgtcg agcaggacgc gcatgggcga cggagtgtag gtcgggtgcg agactcaccc
     2281 cgatgcgcgt cgcgatcccc ctggagcagt gctgggccga ggtgccgggt ggcacggcgc
     2341 gcaccatgat cgacctggcc ggtgccctcg atgcccgggc ggacgtggag gtggtcggcg
     2401 tcgccgcccg ccacgacggc ccgccacccc cggcctggcg t
//
LOCUS       DATISU010000253         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_899702, whole genome shotgun sequence.
ACCESSION   DATISU010000253 DATISU010000000
VERSION     DATISU010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899702"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..909)
                     /locus_tag="VLR27_10125"
     CDS             complement(82..909)
                     /locus_tag="VLR27_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HSP03848.1"
                     /translation="MIPAAFDYVRAGSADEAISLLGEHGDEAKILAGGHSLVPLMKLR
                     LATPSVLVDVGRVDDLKYIRDEGDHVAIGALTRHREVEVSDVLRRDVPILAHATSHVG
                     DPQVRHRGTIGGSLAHGDPASDLPAVLLALGATLVVQGADGTREVAADDFFQGFLETA
                     VGEAELLTEIRVPKATGAGWSFQKFNRRAQDWAIVGVACVRRGDDQVGVGLVNMDSRP
                     VHAAATEAAIASGASVEDAASVADEGTEPAADLNASVEFRRHLARVLTGRALAEASS"
     gene            complement(906..1397)
                     /locus_tag="VLR27_10130"
     CDS             complement(906..1397)
                     /locus_tag="VLR27_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSP03849.1"
                     /translation="MEISITVNGSEARHDVEPRTLLVHHIREALGLTGTNVGCDTSSC
                     GACTVHLDGEAVKSCTVLAVQADGCTLTTIEGLAPSEEQLHPMQQAFREQHGLQCGFC
                     TPGMVMAATSLLDEIPDPTEAQVREGLEGNLCRCTGYHNIVQAVLAAAKSGGGGTKVA
                     VQQ"
     gene            1552..2022
                     /locus_tag="VLR27_10135"
     CDS             1552..2022
                     /locus_tag="VLR27_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03850.1"
                     /translation="MGWFRSTRGASDEAASSYEERLALIRPGVPGRCPACDGLGYIDS
                     IDIGHRYQIQHCKDCLHRWEYLFDGDGVVVGLTELDDAGRPVGRSRVRPLRAAPPETD
                     PEPDVVLDLRDDALADAAEPVAPAEAVEPVVGAAAPTEATELSPAEWLRRSLHR"
     gene            complement(2032..>2310)
                     /locus_tag="VLR27_10140"
     CDS             complement(2032..>2310)
                     /locus_tag="VLR27_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSP03851.1"
                     /translation="RYDDDGNPQTANLADYTMISAAELPSFELVSMETATPLNPLGAK
                     GIGESGTIGSTPAVQSAVCDALSPLGVRHVDMPCTPERVWDAIAAARG"
BASE COUNT          297 a          800 c          889 g          324 t
ORIGIN      
        1 tggcgggcgt cgcgcagggg gatgaccgtc gccgccggga tgaggtcgcc ctcggtgggc
       61 aggaaggtca tggggtggcg gtcagctgct ggcctcggcc agcgcccgtc cggtgagcac
      121 gcgggccagg tggcggcgga actcgaccga cgcgttgagg tcggcggcgg gctcggtgcc
      181 ctcgtcggcc acggacgcgg cgtcctcgac cgacgcgccg gaggcgatgg cggcctcggt
      241 ggcggcggcg tggaccgggc ggctgtccat gttcaccaga ccgacgccga cctggtcgtc
      301 gccgcgccgc acgcaggcga cgccgacgat ggcccagtcc tgggcacggc ggttgaactt
      361 ctggaaggac cagccggccc ccgtggcctt cggcacccgg atctcggtga gcagctcggc
      421 ctcgccgacc gcggtctcga ggaagccctg gaagaagtcg tcggcggcga cctcgcgggt
      481 gccgtccgca ccctgcacga ccagcgtggc gccgagcgcg aggaggacgg cgggcaggtc
      541 ggaggccggg tcgccgtggg cgagcgagcc cccgatggtg ccccggtggc ggacctgggg
      601 gtcgccgacg tgcgaggtgg cgtgggccag gatcggcacg tcgcgccgga gcacgtcgct
      661 gacctcgacc tcgcggtggc gggtgagggc gccgatggcg acgtggtcgc cctcgtcgcg
      721 gatgtacttc aggtcgtcga cccggccgac gtcgacgagc accgacgggg tggccaggcg
      781 cagcttcatc agcggcacga gcgagtggcc gccggcgagg atcttggcct cgtcgccgtg
      841 ctcgcccagc agggagatgg cctcgtcggc cgaaccggcc cggacgtagt cgaacgcggc
      901 ggggatcact gctgcaccgc caccttcgtg ccaccgccgc cggacttcgc ggcggcgagg
      961 acggcctgga cgatgttgtg gtagccggtg cagcggcaca ggttgccctc gagcccctcg
     1021 cgcacctggg cttcggtggg gtcggggatc tcgtcgagca gcgatgtggc cgccatgacc
     1081 atgcccgggg tgcagaagcc gcactgcagg ccgtgctgct cccggaacgc ctgctgcatc
     1141 gggtgcagct gctcctcgga gggagccagg ccctcgatgg tggtgagcgt gcacccgtcg
     1201 gcctggacgg ccagcacggt gcaggacttc accgcttcgc catcgaggtg caccgtgcac
     1261 gcgccgcacg acgaggtgtc gcagcccacg ttggtcccgg tgaggcccag cgcctcgcgg
     1321 atgtggtgga cgagcagcgt gcggggctcg acgtcgtgtc gtgcctcgct cccgttgacg
     1381 gtgatcgaga tctccatccc gtcacccccc tttcctgaca gaccccggtg cctgcggtgg
     1441 cgacctgtcg actgcctagc gcatcgacca tggaagacgt ggacggtgta gtggcactac
     1501 catcgacacc agctgccgca cgggtccggt ggccagagcg agaggttcgg gatgggttgg
     1561 ttccggagca cgcggggtgc cagcgacgag gctgcgtcgt cctacgagga gcgcctggcg
     1621 ctgatccgac ccggcgtccc gggtcgctgt ccggcgtgcg acggcctggg ctacatcgac
     1681 agcatcgaca tcgggcaccg ctaccagatc cagcactgca aggactgcct gcaccgctgg
     1741 gagtacctct tcgacggcga cggcgtcgtc gtggggctca ccgagctcga cgacgccggc
     1801 cgtccggtgg gccgctcccg ggtccgcccg ctgcgggccg ctcccccgga gaccgacccc
     1861 gagccggacg tcgtcctcga cctgcgcgac gacgcgctgg cggacgcggc cgaaccggtc
     1921 gcgccggcgg aggcggtcga acccgtcgtc ggcgccgcag cgccgaccga ggcgaccgag
     1981 ctcagtccgg cggagtggct ccgccgctcg ctgcaccgct gagccagggg ctcagccccg
     2041 ggcggcggcg atcgcgtccc agacccgctc gggcgtgcac ggcatgtcca cgtggcggac
     2101 gccgagcggg gacagggcgt cgcacaccgc ggactgcacg gccggggtcg acccgatcgt
     2161 gcccgactcg ccgatgccct tggcgccgag cgggttgagc ggcgtggccg tctccatgct
     2221 gaccagctcg aagctcggca gctcggccgc cgagatcatc gtgtagtcgg cgaggttggc
     2281 ggtctggggg ttgccgtcgt cgtcgtagcg
//
LOCUS       DATISU010000254         6245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_665992, whole genome shotgun sequence.
ACCESSION   DATISU010000254 DATISU010000000
VERSION     DATISU010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665992"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /gene="trpC"
                     /locus_tag="VLR27_10145"
     CDS             <1..796
                     /gene="trpC"
                     /locus_tag="VLR27_10145"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HSP03852.1"
                     /translation="QPAMATYLDGILLAHRLTAAGDDRDEGELRRAVEGAEPTRGFAQ
                     ALRAADGLAVIAEVKRRSPSKGDLFADLDPARLAGQYEQGGATCLSVLTDEVHFGGSP
                     EDLAAARAGTRLPVLRKDFTVSVKDVLDARLMGADCVLLIAAALDDDELAAFHRTATE
                     VGLDALVEVHDEAELERALAVGATLVGVNPRDLVTFEVDHERAVRMAAAFPDHLVSVA
                     ESGVRGPNDAKALADAGYDAVLVGESLVTSGDPAAAVAALRSATHP"
     gene            911..1561
                     /locus_tag="VLR27_10150"
     CDS             911..1561
                     /locus_tag="VLR27_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HSP03853.1"
                     /translation="MFVKICATTSAADAELAVDAGADAIGVIFAPSRRQVSVDEAKEI
                     VAAVPDHVLTVGVFRDHLAREVLEIVDEVGLRAAQLHGSETPATSAQVHERVPVLIRG
                     MAAGDPQLDEIDRHGADILLLDAPTPGGGEPFDWSLVGDLTRRHRVLLAGGLRPDTVA
                     EAIRTVRPWGVDVASGVEASFGKKDPDAVRAFVTAAKAADPGTDSHAPLADLWTAP"
     gene            1558..2742
                     /gene="trpB"
                     /locus_tag="VLR27_10155"
     CDS             1558..2742
                     /gene="trpB"
                     /locus_tag="VLR27_10155"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HSP03854.1"
                     /translation="MTTTPAAADSIMGDVADGRFGRFGGQFVPETLVGACQEVEHEFR
                     DAWADEAFRARLDGLLRDYAGRPSPLTVCDRLSDELGLRLLLKREDLNHTGSHKINNV
                     LGQALLAQRMGKKRLVAETGAGQHGVATATAAALLGLDCVVYMGAVDMERQKLNVFRM
                     RLLGAEVRSAETGSRTLKDAVNEAMRDWVATVESTHYCLGSAMGPHPYPFMVRELHRV
                     IGTEAREQCLALDGRDPDVVTACVGGGSNAIGIFSGFVDTDAELVGVEPAGGAAVGRG
                     VAGIVHGSTSFLLQDDHGQVQEAESISAGLDYPGVGPEHAHLAELGRARYESVTDAEV
                     LDAFQLLSRTEGIIPALEPAHALAWVSRAREELAGKTVLLNLSGRGDKDVAQVAEILG
                     EL"
     gene            2742..3518
                     /gene="trpA"
                     /locus_tag="VLR27_10160"
     CDS             2742..3518
                     /gene="trpA"
                     /locus_tag="VLR27_10160"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HSP03855.1"
                     /translation="MGTLEKHLRARRDSGRKLLMPYVTGGITDDWLDVIRAYDDAGAD
                     GVEVGIPFSDPVMDGPTIQAANEQALRGGATPISILSELQGLDVSIPLTVMTYANIPF
                     RMGYARFVDECVRAGVHGAIIPDVPLEEMDAWEDAVGDADVDTVMLASPISSDDRLAR
                     LTARSKGFVYGVNVLGVTGERSDVGTWTADLAGRLKQVTDLPVVMGFGISSPETAAAA
                     AAPSDGVIVASAIMRLLLDGGSALEAGAFVADLRRAIDGV"
     gene            complement(3405..4409)
                     /locus_tag="VLR27_10165"
     CDS             complement(3405..4409)
                     /locus_tag="VLR27_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HSP03856.1"
                     /translation="MNPGAPDPDPPIRERAVRVLRRMAVEWREDRVPDAAAGVAFYAV
                     LSLLPAFLTLAAMLGPLDSLIGGEVAERVQERVLDFLGTVLTAEADGTLEVARDLFEN
                     ERPGLLTFSLVVAIWTVSRSFAALVRALDVVYDLDEHRSWVRVRGTALIVAVGSIVAA
                     SLMLVAIVVGPLFGTGEQIADQIGLGDQFVFLWDVFRLPFAFLLLVLWAATIFHIAPN
                     HHTPWRWDLPGALLTAVLWVALSGALRLYLEVTQAGNAVFGALGGALIVLLWFWLLSL
                     AVLIGGELNQVLADELGVDLRSDPVDGATEVGDERTGLQRGPAVEEQAHDRGGDDHPV
                     "
     gene            complement(4406..5050)
                     /locus_tag="VLR27_10170"
     CDS             complement(4406..5050)
                     /locus_tag="VLR27_10170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSP03857.1"
                     /translation="MATPHSTSAYADAPKSGSGPGVLVLHSWWGLNDAIKDRCNQLAD
                     AGFTVVAPDLFDGEVVRDDAHGEKILAEADANRLVLGVQSTADALHRMPATEGSRIMV
                     VGFSMGASLGLWFSERFPDQVSAVVGYYGTQSIDFKATMSAYQLHLAEDDAMVDPDEL
                     ALMEASFGLAGRPVEVHTYQGVAHHFAEAGTPNYDAEAADLAWQRTIEFLRRHA"
     gene            5122..5424
                     /locus_tag="VLR27_10175"
     CDS             5122..5424
                     /locus_tag="VLR27_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03858.1"
                     /translation="MGAPDHDPDCELCEAARLTPWFHEDELCWVAECEACAVPMVVWR
                     PHGIDPTEDDQERMIDRLHAVADVQLGEHWLDPDRRSIPGHWHVHARPKGRFNGWT"
     gene            5457..>6245
                     /locus_tag="VLR27_10180"
     CDS             5457..>6245
                     /locus_tag="VLR27_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSP03859.1"
                     /translation="MTELLERSARYIDEGTYEGPAANNPTDASFHEIADGIGMVAAFS
                     HVWGLRSGGELAVFDTSLAPFGALAVDALRRWTEDPLAHIVYTHGHVDHVGGTPAFLA
                     DAESRGHARPRITSHENAPARFARYQDTHGYNAVINARQFGPGGLQFPAQFVAPDVPF
                     DVDLSLTVGDTVVEIHHDRGETDDHAWSWVPEHKAIFCGDLFIWVFPTAGNPQKVQRY
                     AADWAVALRKMIALEPELLLPAHGLPIEGKERIATVLDNTATALE"
BASE COUNT          897 a         2382 c         2153 g          813 t
ORIGIN      
        1 gcagccggcc atggcgacct acctcgacgg gatcctgctc gcccaccggc tgaccgctgc
       61 cggcgacgac cgggacgagg gcgagctccg gcgtgccgtc gagggcgccg agcccacccg
      121 cggcttcgcc caggccctcc gagccgccga cggcctcgcc gtcatcgccg aggtcaagcg
      181 ccggtccccg tcgaagggtg atctcttcgc cgacctcgac ccggcccggc tcgccggcca
      241 gtacgagcag ggcggggcca cctgcctgtc ggtgctcacc gacgaggtcc acttcggcgg
      301 ctcacccgag gacctcgccg ctgcccgagc cggcacccgc ctgcccgtgc tgcgcaagga
      361 cttcaccgtg tcggtcaagg acgtgctcga cgcccggctc atgggcgccg actgcgtgct
      421 gctcatcgcc gccgccctcg acgacgacga gctggccgcc ttccaccgca ccgccaccga
      481 ggtcggcctc gacgcgctgg tcgaggtgca cgacgaggcc gagctcgagc gggcgctcgc
      541 cgtcggcgcc accctcgtcg gcgtcaaccc gcgggacctc gtcaccttcg aggtcgacca
      601 cgagcgggcc gtgcgcatgg ccgccgcgtt ccccgaccac ctcgtctccg tcgccgagtc
      661 cggcgtccgc ggccccaacg atgccaaggc gctcgccgac gccggctacg acgccgtcct
      721 ggtgggggag tccctcgtca cctccggcga ccccgccgcc gccgtcgccg ccctccgctc
      781 cgccacccac ccctgaaccg ttctggcata catccgtgac caccaggtgg cacggatgta
      841 ggccagaacg aagtggggtg gggcggatcg cgctgcagga tcggggcggg accccggtac
      901 ggtcgcggcc gtgttcgtga agatctgcgc caccaccagc gccgctgacg ccgagctggc
      961 cgtcgacgcc ggtgccgacg cgatcggcgt catcttcgcc ccctcccgcc ggcaggtgag
     1021 cgtcgatgag gccaaggaga tcgtggccgc cgtgcccgac cacgtgctca ccgtcggcgt
     1081 gttccgcgac cacctggccc gcgaggtcct cgagatcgtc gacgaggttg gcctgcgggc
     1141 cgctcagctc cacgggtccg agacgccggc caccagcgcg caggtccacg agcgggtccc
     1201 ggtgctgatc cggggcatgg ccgccggcga cccccagctc gacgagatcg accgccacgg
     1261 cgccgacatc ctcctcctcg atgccccgac gcccggcggc ggcgagccct tcgactggag
     1321 cctggtcggc gacctcaccc gtcgccaccg ggtgctcctc gccggcggcc tccggcccga
     1381 caccgtcgcc gaggccatcc gcaccgtccg cccctggggg gtcgacgtcg cctccggggt
     1441 cgaggcctcc ttcgggaaga aggaccccga cgccgtccgg gccttcgtca ccgccgccaa
     1501 ggccgccgac cccggcaccg actcccacgc ccccctcgcc gacctctgga ccgcaccatg
     1561 accaccactc ccgccgccgc cgactcgatc atgggcgacg tggccgacgg ccgcttcggc
     1621 cgcttcggcg gccagttcgt ccccgagacc ctggtcggcg cctgccagga ggtcgagcac
     1681 gagttccgcg acgcctgggc cgacgaggcg ttccgggccc ggctcgacgg cctgctccgc
     1741 gactacgccg gccgccccag cccgctgacg gtgtgcgacc ggctctccga cgagctcggc
     1801 ctgcgcctgc tgctcaagcg ggaggacctg aaccacacgg gcagccacaa gatcaacaat
     1861 gtcctcggcc aggccctcct cgcccagcgc atgggcaaga agcgcctggt ggccgagacc
     1921 ggggccggcc agcacggcgt ggccaccgcc accgcggcgg ccctcctcgg cctcgactgc
     1981 gtcgtctaca tgggcgccgt cgacatggag cgccagaagc tcaacgtgtt ccgcatgcgc
     2041 ctgctcggcg ccgaggtccg ctcggccgag accggctcac gcaccctcaa ggacgcggtg
     2101 aacgaggcca tgcgcgactg ggtcgccacc gtcgagtcca cgcactactg cctcggctcg
     2161 gccatgggcc cgcacccgta cccgttcatg gtccgcgagc tgcaccgggt catcggcacc
     2221 gaggcccgcg agcagtgcct cgccctcgac ggccgcgatc ccgacgtcgt cactgcctgc
     2281 gtcggcggcg gctccaatgc catcggcatc ttctccggct tcgtcgacac cgacgccgag
     2341 ctggtcgggg tcgagcccgc cggcggcgcc gccgtcggtc ggggcgtggc cggcatcgtc
     2401 cacggctcga cgtcgttcct gctccaggac gaccacggcc aggtccagga ggccgagtcg
     2461 atctccgccg gcctcgacta cccgggcgtc ggccccgagc acgcgcacct ggccgagctc
     2521 ggccgggccc ggtacgagtc ggtcaccgac gccgaggtgc tcgacgcgtt ccagctgctg
     2581 tcccgcaccg agggcatcat cccggcgctc gagccggccc acgccctggc ctgggtcagc
     2641 cgggcccgcg aggagctggc cggcaagacc gtgctgctca acctgtccgg ccgcggcgac
     2701 aaggacgtcg cccaggtggc cgagatcctc ggggagctgt gatgggcacg ctcgagaagc
     2761 acctgcgcgc ccgccgcgac agcggtcgca agctgctcat gccctacgtc accggcggca
     2821 tcaccgacga ctggctcgac gtcatccggg cctacgacga cgccggcgcc gacggggtcg
     2881 aggtcgggat cccgttctcc gacccggtca tggacggccc caccatccag gccgccaacg
     2941 agcaggccct gcggggcggg gccacgccca tcagcatcct gtccgagctg cagggcctcg
     3001 acgtgtcgat cccgctcacc gtcatgacct acgcgaacat cccgttccgc atgggctacg
     3061 cccgcttcgt cgacgagtgc gtccgggccg gtgtgcacgg ggcgatcatc cccgacgtgc
     3121 cgctcgagga gatggacgcc tgggaggacg ccgtcggcga cgccgacgtc gacacggtca
     3181 tgctggccag cccgatcagc agcgacgacc gtctcgcccg cctcacggcc cgcagcaagg
     3241 gcttcgtcta cggcgtgaac gtgctcggcg tgacggggga gcgctccgac gtgggcacct
     3301 ggaccgccga cctcgccgga cggctcaagc aggtcaccga cctcccggtc gtgatgggct
     3361 tcggcatctc ctcgccggag accgcagccg cggcagcagc gccgtcagac ggggtgatcg
     3421 tcgcctccgc gatcatgcgc ctgctcctcg acggcgggtc cgcgctggag gccggtgcgt
     3481 tcgtcgccga cctccgtcgc gccatcgacg gggtctgagc gcaggtccac gccgagctcg
     3541 tcggccagca cctggttgag ctcgccgccg atcagcacgg cgagcgacag cagccagaac
     3601 cacagcagca cgatcagcgc accgccgagg gcgccgaaca ccgcgttccc ggcctgcgtg
     3661 acctccaggt acaggcgcag cgcgcccgac agggccaccc acagcaccgc ggtgaggagc
     3721 gcgccgggca ggtcccagcg ccacggcgtg tggtggttgg gggcgatgtg gaagatggtc
     3781 gccgcccaca gcacgaggag gaggaaggcg aaggggagcc ggaagacgtc ccacaggaac
     3841 acgaactgat cgccgaggcc gatctggtcg gcgatctgct caccggtgcc gaacagcggg
     3901 ccgacgacga tggcgacgag catgagcgac gctgcgacga tcgagccgac ggcgacgatc
     3961 agggcggtgc cccggacccg cacccagctg cggtgctcgt ccaggtcgta gaccacgtcg
     4021 agcgcccgca cgagggcggc gaagctgcgt gacaccgtcc agatcgcgac gacgagcgag
     4081 aacgtgagca ggcccggtcg ctcgttctcg aacaggtcgc gggccacctc gagggtgccg
     4141 tccgcctcgg ccgtgagcac ggtgccgagg aagtcgagca cccgctcctg cacccgctcg
     4201 gcgacctcgc cgccgatcag ggagtcgagc gggcccaaca tggccgccag ggtcaggaac
     4261 gccggcagca ggctgagcac ggcatagaag gccacgccgg cagctgcgtc gggaacgcgg
     4321 tcctcacgcc actcgacggc catccggcgg agcacccgga ccgcccgctc ccggatgggg
     4381 ggatcggggt cgggggcgcc cgggttcatg cgtggcgacg gaggaactcg atggtccgct
     4441 gccaggcgag gtcggccgcc tcggcgtcgt agttcggcgt gcccgcttcg gcgaagtggt
     4501 gggcgacgcc ttggtaggtg tggacctcga ccggccggcc ggcgagcccg aagctggcct
     4561 ccatgagcgc cagctcgtct ggatcgacca tggcgtcgtc ctcggccagg tgcagctggt
     4621 aggcggacat ggtcgccttg aagtcgatgc tctgggtgcc gtagtagccc acgacggcgg
     4681 acacctggtc ggggaagcgc tcggagaacc acaggccgag cgaggcaccc atggagaacc
     4741 cgacgaccat gatgcggctg ccctcggtgg ccggcatgcg gtggagcgca tcagcggtgg
     4801 actgcacccc gagcacgagc cggttggcgt cggcctcggc caggatcttc tcgccgtggg
     4861 cgtcgtcgcg gacgacctcc ccgtcgaaca ggtcgggggc gaccacggtg aagccggcgt
     4921 cggccagctg gttgcaccgg tccttgatcg cgtcgttcag gccccaccac gagtgcagga
     4981 cgaggacgcc cggtccgctc ccggacttgg gcgcgtcggc gtaggcggag gtggagtggg
     5041 gggtcgccac aggaccgacg ctaccggcac cctcctcagg aactggcagt tgccctgtag
     5101 cgcgggcagg ctccatggcc gatgggcgcc ccggaccacg atcccgactg cgagctgtgc
     5161 gaggccgcgc ggctgacgcc gtggttccac gaggacgagc tgtgctgggt ggccgagtgc
     5221 gaggcgtgcg cggtgccgat ggtggtgtgg cgcccccacg gcatcgaccc gaccgaggac
     5281 gaccaggagc gcatgatcga ccggctccac gccgtcgccg acgtccagct cggcgagcac
     5341 tggctggacc cggaccgccg ctcgatcccc gggcactggc acgtgcacgc ccgcccgaag
     5401 ggccggttca acggctggac ctgagcgcgt tccgtggtcg ctgccagaat cggacgatga
     5461 ccgagctgct ggagcgctcc gcccgctaca tcgacgaggg cacctacgag ggcccggccg
     5521 ccaacaaccc caccgacgcc tccttccacg agatcgccga cgggatcggc atggtggcgg
     5581 cattctccca cgtgtggggc ctgcggtcgg gcggcgagct ggccgtgttc gacacctcgc
     5641 tggccccctt cggtgcgctg gcggtcgatg cgctccgccg gtggaccgag gacccgctcg
     5701 cccacatcgt ctacacgcac ggccacgtcg accacgtggg cggcacgccg gcgttcctgg
     5761 ccgacgccga gtcccgcggt cacgcccggc cccgcatcac ctcgcacgag aacgccccgg
     5821 cccgcttcgc ccgctaccag gacacccacg gctacaacgc ggtgatcaac gcccggcagt
     5881 tcggtcccgg cggcctgcag ttccccgccc agttcgtcgc ccccgacgtc cccttcgatg
     5941 tggacctgtc gctgacggtc ggcgacaccg tggtcgagat ccaccacgac cggggcgaga
     6001 ccgacgatca cgcctggtcc tgggtccccg agcacaaggc gatcttctgc ggggacctgt
     6061 tcatctgggt gttccccacc gccggcaacc cccagaaggt gcagcgctac gcggccgact
     6121 gggccgtggc gctgcgcaag atgatcgccc tcgagccgga gctgctcctg cccgcccacg
     6181 gcctgccgat cgaaggcaag gagcgcatcg ccacggtgct cgacaacacc gccaccgcgc
     6241 tcgag
//
LOCUS       DATISU010000255         4686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_154399, whole genome shotgun sequence.
ACCESSION   DATISU010000255 DATISU010000000
VERSION     DATISU010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154399"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="VLR27_10185"
     CDS             <1..1110
                     /locus_tag="VLR27_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HSP03860.1"
                     /translation="RILEQLLASAEQRLREEGIRGVIYLFKNNTDSAGNSYGCHENYL
                     TSRNDDLGSYSEHLIPFLVSRQIYAGAGKVLQTARGAVFCLAQRAEHIWEGVSSATTR
                     SRPIINTRDEPHADAERYRRLHVIVGDSNMNEYTNYLKVGTTAILLRMLEDPNVVMRD
                     MTMENPIRAIREISHDITCQRKVRLANGRELSALEIQQEYLDKALTYAARKGLPEQEQ
                     RALDMWQHVMHGLSTDPLSLSRECDWVTKHHLIERYRKKHDLALNSPRVALIDLQYHD
                     VNRERGLFYKMQNAGLVERICDDDAIDEAIETPPQTTRAKLRGDFIRRAKERKRDYTV
                     DWVHLKLNDQAQRTVLCKDPFKSSDDRVDRLIASL"
     gene            1121..2137
                     /locus_tag="VLR27_10190"
     CDS             1121..2137
                     /locus_tag="VLR27_10190"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HSP03861.1"
                     /translation="MPSFETAEVTEVLAERDGLQRVDTTAGRAYALTQLTGSVAVGDR
                     VVLNTTAVGLGLGTGGWHVVHWNLSREHLDLPGPGHIMKVRYTSLQCDTGAAEEDHDV
                     PTDLAGTPVVVGTLHSQVPLVAAAYLHHHPEARVAYVMTDGAALPMALSDLVVGMRRA
                     GLVHGTVTAGHAFGGDLEAVSTPSALTLAVHTLDADLVIAAMGPGVVGTGAPLGTTAV
                     EAAGVLDAAAALGADPILCVRASEADARDRHRGVSHHVASVLDLTRSRVTVPTVLGAE
                     LGREDHDVVVTEPPPIAEWLDARDLWVTTMGRNVFEDPLFFAAGAAAGVVAAHRPGRR
                     RDHG"
     gene            2151..3089
                     /locus_tag="VLR27_10195"
     CDS             2151..3089
                     /locus_tag="VLR27_10195"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HSP03862.1"
                     /translation="MAVPKIERLLDLIAELLHAERPLTAEQLHERIPGYPPDKDSFKR
                     AFERDKKDLRDEGVPLRIETVPGSTPPQDGYRIDPDEYYLRDPGLDADELAAIHLAAS
                     AVRLEGASAAGGLRKLGAPLRGGTASTIATLPTAPHLSTAFDAVAAGRRLEFRYGGKD
                     RTVDPFRLQHQRGRWYLQGHDAGAGEARSFRLDRVEGDMVAGEAGSAEVDLDPADNPL
                     QLDGWALGDDEPVEARVRIAPRQARVAVGQVGPAVPAEWEDDGSVVLTLPVRRPEGFR
                     SFVLGFLDDAEVLEPAELRDDLVAWLRAVVGDGGGA"
     gene            3089..4045
                     /locus_tag="VLR27_10200"
     CDS             3089..4045
                     /locus_tag="VLR27_10200"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HSP03863.1"
                     /translation="MARPSATDRLGRILAIVPWIAEQEAPLVADVCARFGLTPEELTA
                     DLEIVPMVGLYPYTPDNLIELIFDDDRIRLEYGNFFERPLRLTKEEALVILAAAKASV
                     PATGHDPAGPLSRALDKIADALGVDLDDDLEVVLAEPGGDQREVLEAAVRDREALDIG
                     YFSHARNEHTQRTIEPLRLFHSEGAWYLDAHCRTTPGDRVFRVDRITDIHATGEHFER
                     TLDRTSTEAFSPAEDLPRVTLEVDADGAWVADQYPVEERTDLEGGGLRVTLVVSARPW
                     LERLLLQLGPHGRVVEAPDDLADAGAEAAGRLLARYGAGTSR"
     gene            4078..4686
                     /gene="lepB"
                     /locus_tag="VLR27_10205"
     CDS             4078..4686
                     /gene="lepB"
                     /locus_tag="VLR27_10205"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HSP03864.1"
                     /translation="MTETTDPGAGPADHPDSGDASDAGRSGMSRSTRSAIEWVAVIVG
                     ALVVALVVKTFLIQAFYIPSESMVPSLKVGDRVLVNKLSYTTGDIERGDVVVFARPGG
                     PGSDGIEDLIKRVVGLPGETVEGRDGRVYIDGEALPETYLPDGVETSVFPPYEVPEDH
                     VWVMGDNRGASDDSRRFKAVPTSDVVGRAFVIIWPLTELGTL"
BASE COUNT          697 a         1773 c         1620 g          596 t
ORIGIN      
        1 cgcatcctcg agcagctcct ggccagcgcc gagcagcgcc tccgcgagga gggcatccgc
       61 ggggtcatct acctgttcaa gaacaacacc gactcggccg gcaactccta cggctgccac
      121 gagaactacc tgaccagccg caacgacgac ctcggctcct actccgagca cctcatcccg
      181 ttcctggtca gccgccagat ctacgccggc gccggcaagg tcctccagac cgcccggggc
      241 gcggtgttct gcctggccca gcgggccgag cacatctggg agggcgtcag ctccgccacc
      301 acccgcagcc ggccgatcat caacacccgc gacgagcccc atgccgacgc cgagcgctac
      361 cgccgcctgc acgtcatcgt cggcgactcg aacatgaacg agtacacgaa ctacctcaag
      421 gtcggcacca cggcgatcct gctgcgcatg ctggaggacc cgaacgtggt gatgcgggac
      481 atgaccatgg agaacccgat ccgggccatc cgcgagatca gccacgacat cacctgccag
      541 cgcaaggtgc gcctggccaa cgggcgcgag ctgtcggccc tggagatcca gcaggagtac
      601 ctcgacaagg cgctcacgta cgcggcccgg aagggcctgc ccgagcagga gcagcgggcc
      661 ctcgacatgt ggcagcacgt catgcacggc ctcagcaccg acccgctgtc gctgagccgg
      721 gagtgcgact gggtgaccaa gcaccacctc atcgagcgct accgcaagaa gcacgacctg
      781 gcgctgaact cgccccgggt ggccctcatc gacctgcagt accacgacgt gaaccgcgag
      841 cggggcttgt tctacaagat gcagaacgcc ggcctggtcg agcggatctg cgacgacgac
      901 gccatcgacg aggccatcga gacgccaccg cagaccacgc gggccaagct ccgtggcgac
      961 ttcatccggc gggccaagga acgaaagcgc gactacacgg tggactgggt ccacctgaag
     1021 ctgaacgacc aggcccagcg cacggtgctg tgcaaggacc cgttcaagtc gagcgacgat
     1081 cgcgtcgacc ggctcatcgc cagcctctga gcgagcgggc gtgcccagct tcgagacggc
     1141 cgaggtcacc gaggtgctcg ccgagcgcga cggcctccaa cgggtcgaca ccacggcggg
     1201 tcgggcgtac gcgctcaccc agctcaccgg ctcggtcgcg gtgggggacc gggtggtgct
     1261 caacaccacc gcggtcggcc tcgggctcgg cacgggcggg tggcacgtcg tgcactggaa
     1321 cctgtcgcgt gagcacctgg acctgcccgg ccccggccac atcatgaagg tccggtacac
     1381 atcgctgcag tgcgacaccg gcgccgccga ggaggaccac gacgtcccca ccgacctggc
     1441 cggcacgccc gtggtcgtcg ggaccctcca cagccaggtg cctctcgtgg cggccgccta
     1501 cctgcaccac caccccgagg cacgggtcgc ctacgtcatg accgacgggg ccgcgctccc
     1561 gatggcgctc tccgacctgg tggtcgggat gcgtcgcgcc ggcctggtgc acggcaccgt
     1621 gaccgctggc cacgcgttcg gtggcgacct cgaggcggtg tcgacgccct cagcgctcac
     1681 cctcgcagtg cacaccctcg atgccgacct cgtcatcgcc gccatgggcc ccggggtcgt
     1741 gggtacgggc gcccccctcg gcaccacggc ggtcgaggcc gccggggtcc tcgacgccgc
     1801 agctgccctc ggcgcggacc cgatcctctg cgtgcgcgcg tccgaggccg acgcccgcga
     1861 ccggcaccgc ggcgtgagcc accacgtggc gagcgtcctc gacctcaccc gctcccgggt
     1921 cacggtgccg accgtgctcg gcgccgagct cggccgagag gaccacgacg tggtggtcac
     1981 cgagccgccg ccgatcgccg agtggctcga cgcccgcgac ctgtgggtca ccaccatggg
     2041 gcggaacgtc ttcgaggacc cgctgttctt cgccgcgggt gccgcggccg gtgtggtggc
     2101 ggcgcatcgt cccgggcggc gccgggacca cgggtagcct cgccgtcccc atggccgtcc
     2161 cgaagatcga acgcctcctc gacctcatcg ccgagctgct gcacgcggag cgtcccctga
     2221 cggcggagca gctccacgag cgcatccccg gctacccgcc ggacaaggac tcgttcaagc
     2281 gggccttcga gcgggacaag aaggacctgc gcgacgaggg ggtcccgctg cggatcgaga
     2341 cggtgccggg ctccacgccg ccccaggacg gctaccgcat cgaccccgac gagtactacc
     2401 tgcgcgaccc cggcctggac gccgacgagc tggcggccat ccacctggcg gcgagcgccg
     2461 tgcggctgga gggtgcgagc gccgccggag ggctgcgcaa gctgggcgcg ccgctgcggg
     2521 gcgggaccgc ctccaccatc gccaccctcc ccaccgcacc gcacctgagc acggcgttcg
     2581 acgcggtggc ggccggtcgt cgcctggagt tccgctacgg cggcaaggac cgcacggtcg
     2641 atccgttccg gctccagcac caaaggggtc gctggtacct gcagggccac gacgccggcg
     2701 ccggcgaggc ccgctcgttc cggctcgacc gggtggaggg cgacatggtc gccggggagg
     2761 cgggcagcgc cgaggtcgac ctggacccgg ccgacaaccc gctgcagctc gacggctggg
     2821 cgctcggcga cgacgagccc gtcgaggccc gcgtccgcat cgccccgcgc caggcccggg
     2881 tcgccgtcgg ccaggtcggc ccagccgtcc ccgccgagtg ggaggacgac gggtcggtgg
     2941 tcctcacgct gccggtgcga cggcccgagg gcttccgctc cttcgtcctc ggcttcctcg
     3001 acgacgccga ggtgctcgag ccggctgagc tgcgcgacga cctggtggcg tggctgcgcg
     3061 ccgtcgtcgg cgacgggggt ggcgcctgat ggcccgcccc agcgccaccg accgcctcgg
     3121 ccggatcctc gccatcgtgc cctggatcgc cgagcaggag gcccccctcg tggccgacgt
     3181 ctgcgcccgg ttcggcctca cgcccgagga gctcaccgcc gacctcgaga tcgtcccgat
     3241 ggtgggcctg tacccctaca cgcccgacaa cctgatcgag ctgatcttcg acgacgaccg
     3301 gatccggctg gagtacggga acttcttcga gcggcccctc cggctgacca aggaggaggc
     3361 actggtcatc ctggcggcgg cgaaggcttc ggtgccggcg accgggcacg acccggccgg
     3421 cccgctgtcc cgggcgctgg acaagatcgc cgacgccctc ggcgtcgacc tcgacgacga
     3481 cctcgaggtc gtgctcgccg aaccaggcgg cgaccagcgc gaggtgctcg aggccgcggt
     3541 gcgcgaccgg gaggcgctcg acatcggcta cttcagccac gcccgcaacg agcacaccca
     3601 gcgcacgatc gagccgctgc ggctgttcca cagcgagggc gcctggtacc tcgacgccca
     3661 ctgccgcacc acgcccggcg accgggtgtt ccgcgtcgac cgcatcaccg acatccacgc
     3721 cacgggcgag cacttcgagc gcaccctgga ccgcacgtcc acggaggcct tcagccccgc
     3781 cgaggacctg ccccgcgtca ccctcgaggt cgatgccgac ggcgcctggg tcgccgacca
     3841 gtacccggtg gaggagcgga ccgacctcga gggcggtggc ctgcgggtga ccctcgtggt
     3901 gagcgcccga ccgtggctcg agcgcctcct gctgcagctc ggcccgcacg gccgggtggt
     3961 cgaggcaccc gacgacctgg cggatgcggg tgccgaggcc gccgggcggc tgctggcccg
     4021 gtacggggcc gggacctcgc gctgaccacc gccacccgcc ggtaccatcg acggactgtg
     4081 accgagacga ccgacccggg cgccgggccc gccgaccacc cggactccgg ggacgcgtcc
     4141 gacgccggcc gctccggcat gtcccggtcc acccgctcgg ccatcgagtg ggtcgcggtc
     4201 atcgtgggtg ccctggtggt ggccctcgtc gtcaagacgt tcctgatcca ggccttctac
     4261 atcccgtcgg agtcgatggt gcccagcctg aaggtgggcg accgggtgct ggtgaacaag
     4321 ctcagctaca ccaccggcga catcgagcgg ggcgacgtcg tcgtcttcgc ccggccgggc
     4381 ggcccggggt ccgacggcat cgaggatctc atcaagcggg tcgtgggcct gcccggcgag
     4441 accgtcgagg gtcgcgacgg gcgcgtctac atcgacggcg aggcgctccc ggagacgtac
     4501 ctgcccgacg gggtggagac gtcggtcttc ccgccctacg aggtgcccga ggaccacgtc
     4561 tgggtgatgg gcgacaaccg gggcgcgtcc gacgactcgc ggcggttcaa ggccgtgccg
     4621 accagcgacg tggtcggccg ggcgttcgtg atcatctggc cgctgaccga gctcggcacc
     4681 ctctag
//
LOCUS       DATISU010000256        11273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_340459, whole genome shotgun sequence.
ACCESSION   DATISU010000256 DATISU010000000
VERSION     DATISU010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 11273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11273
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340459"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VLR27_10210"
     CDS             <1..193
                     /locus_tag="VLR27_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HSP03865.1"
                     /translation="GPVGWVDAAGNGTWAVGIRSAIVDGTVARLFAGVGVVADSDPQA
                     ELAETEAKLQAVLSAIVRP"
     gene            complement(205..570)
                     /locus_tag="VLR27_10215"
     CDS             complement(205..570)
                     /locus_tag="VLR27_10215"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HSP03866.1"
                     /translation="MSHPVLEIAQPGRRPLFVELREPLEIGRECDGVLIADGQASRRH
                     ANLAPRGETIVVEDLGSTNGTHLDGNRISSAVVLMPGAVLQVGDTTVQVVRSSPLGSA
                     RTASANGRTTATPASHVVP"
     gene            complement(584..1006)
                     /locus_tag="VLR27_10220"
     CDS             complement(584..1006)
                     /locus_tag="VLR27_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HSP03867.1"
                     /translation="MSKNTAVDHLSNVPLFSACSKKELAAIAKSSDQLSVGDGHALTT
                     QDESSREAFVILSGKAVVKRNGRKVAELGPGDSVGELGLLDHGVRTATVVVDGPAEVL
                     VIGPREFSALLQDVPSLSKKLLQHLAATVRDLDTKSYG"
     gene            complement(1072..2721)
                     /gene="menD"
                     /locus_tag="VLR27_10225"
     CDS             complement(1072..2721)
                     /gene="menD"
                     /locus_tag="VLR27_10225"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HSP03868.1"
                     /translation="MNDTRGWSPDVQATFCATLVDEWVRCGLTDAVVCPGSRSTPLTV
                     ALASDDRVRTHVHHDERSGSFLALGLAATAGRPVVVVTTSGTAVVQLHAAAVEADLAG
                     VPLILCTADRPPELRDVAAPQTVDQTHLFGRSVRFFTDPGVADPTQASTWRSLAARTW
                     AEATAEWPGPVQLNLPFREPLLGEVGDLPPGRGDGPWHQRLAAGRVAPVLDPTVCAAR
                     GVVIAGRGTADPEAVSALAEDLGWPLLADPSSGCSSTTSIECFDPLLRTAFAERARPD
                     VVIRSGGMPASKVLAQWLDGCDHEDLVIAAPAWWPDPSHTATVLLDAVPVVPDVHEPV
                     EEWVEAWRVAAAAVGTAIDEVLADAPLSEPAVARHLTRTLPPGAVLVTSSSMPVRDVE
                     WFGIARRDLRVLANRGANGIDGVVSTAVGAALVGAKTTLLIGDVAFLHDSNGLLGLAD
                     RDVDLTIVVLDNDGGGIFSFLPQRHELAADRFERLFGTPHGLDLVALARAYGVDADRV
                     VSVEQLEWRPGVRVLVVPTDREENVTLHGRLAEAAVRAVHA"
     gene            2747..3502
                     /locus_tag="VLR27_10230"
     CDS             2747..3502
                     /locus_tag="VLR27_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSP03869.1"
                     /translation="MWLHAEREGSGPPLVLVHGFTQTRRCWGPVAEDLATDHTVVRVD
                     APGHGMSADIMAGLRGGGRLIADQGGEATYLGYSMGGRYLIHLALANPELVRGLVLLG
                     ATAGIDDPDDRAARLRADEATAARIRELGLLEFLQTWIAQPMFAGIPAERQYLLERME
                     NTVDGLESSLLQSGTGAQDPSWDKLHRLDMPVLVVAGERDDKFAALGQRLRGSIGDNA
                     TFATVPGAGHAAHLEQPEAFLDLLRPWLAAHDL"
     gene            complement(3561..6476)
                     /locus_tag="VLR27_10235"
     CDS             complement(3561..6476)
                     /locus_tag="VLR27_10235"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HSP03870.1"
                     /translation="MIISFLVVTAILALGATNLEFATGQDSYLDDDDQIAIDNEALQS
                     QFGGETIILLFQATGDADISDLYAEGNLEELERIESELRAIDEVYSVVAPLTTVQFSE
                     SITTGVAPSGETIEPSPGTGALLSALEREPDEESQAIRQADVTTTLARLGAVEERDLS
                     NPGWIEFLLFGNQNVEVVDGVPEMPTGDDRVVRLSLQSTFPNLQTAVGGVLLQGNADL
                     DTLSSGTQAVLDVAESAQLDGFEVVVTGSPVFLKDINDYLQGGMLTLGAIALAIMAVI
                     LLVMFRVRWRFLPLLSTVVGVLWGFALLGYIGIDLSLVTISGLPILIGLGIDFAIQVH
                     NRIEEEAALDKDEHPISETLANVGPALVAATLAAVVAFMALQLSQVPMIRDFGVMLAV
                     GIVALVFCGIVLPTTILGVREYRSRTGDVAESRVERFVVWLGGLPPKAAVPLVVLSAL
                     LFVGGVAMEGRFEIESDPVRWINQDTQTVRDLDTLEEATGFSSTLSVLVSANNVLDED
                     LTQVLHDFTTYAEGLDEVATSSSLTGTMAKIINIPGATEIAPTSADAAAAAAVMPEDI
                     RKVLLSDDGTATQVNLRLAPGSLEDRADLVEVLEADLDQRIAALDLPEDSILLEDLGP
                     DEPAMRATPAGLAVVGVGLLENLSANRAVLTYLGLALAALFLLIRLQSLGRAALTLVP
                     VGLAIGTSSVVIGAFGLTLSPLTTVSGPLVIASCTEFSVLITARYLEERQSGRSPKEA
                     SDHAARRTGRAFFTSAATTVGGFAVLIGSALPLLRDFGIIVTLNVTVALLAALVVMPP
                     LLMWADERGYFPVEGHAPGAAVRLAAQPQGPRLIAWVVGVLVVAAIAVGLWASAERDA
                     GDSVEIAYTPVALPVTTTTTTAAPEPTGELSLDDYPEEAPEGLVQSTVFNLLTAQGAD
                     PRAANCTHEQLLAGTPESELLAMAGSDQDTLFGLVAESASTCTVPQDVIDAAVAAGL"
     gene            complement(6595..7491)
                     /locus_tag="VLR27_10240"
     CDS             complement(6595..7491)
                     /locus_tag="VLR27_10240"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HSP03871.1"
                     /translation="MTAPTPTGLQAWIAGARVRTLPAAVVPVLVGTAAARGVDGPLIA
                     WRAVAAAVVALALQVATNYANDYSDGIRGTDDPGGRVGPVRLVGWGLKEPSAVKRAAM
                     ASFAVAGAAGLALTLAVGWELLLVGAASMLAGWFYTGGSRPYGYLGLGEVFVFVFFGV
                     VATVGSTYVQVEEVTGLSLLASVPVGLLATALLVTNNLRDIPGDTVAGKQTLAVRLGD
                     ARTRTLFVAMLVVALLLVVPVAIARPAAIVALLAAVPALRPIRLVRSGAAGRDLIAVL
                     GATGQVQLVFGLLLALGLVISA"
     gene            7533..8594
                     /locus_tag="VLR27_10245"
     CDS             7533..8594
                     /locus_tag="VLR27_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP03872.1"
                     /translation="MQRLVAIEARGERFTDALRAAWDAGDAVLPVDPRLPARTASALV
                     DALGGGGPIDAEVALVVPTSGTTGDPKGVELTHAQVAASAEATSSFLGVEPARHRWLA
                     CLPLAHVGGLSVVTRAWHTGTPLEVHDGFDAAAVDAAARAGCTHVSLVGTALARIDAS
                     AWTRIVLGGSAPPPDRPANTVATYGMTETGSGVVYDGVALPGVELRIVDGEILVRGPM
                     VATRYRDGTPVVDADGWLHTGDAGRLDDGVLTVHGRRGDMIITGGENVHPDPVEQRLR
                     EHPAVADAAVVGRPDPEWGQRVVAVLELAPEATVPSLAELRDWVRDALPPWTAPKELE
                     VVDALPRTSLGKVRRAAVR"
     gene            8716..9006
                     /locus_tag="VLR27_10250"
     CDS             8716..9006
                     /locus_tag="VLR27_10250"
                     /inference="COORDINATES: protein motif:HMM:NF017198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HSP03873.1"
                     /translation="MSTYEPQPSQLHSPAWVAVTKIQFAVSVLALVIGLAYLPVEPWI
                     VAYLVMGTLLLTSSTVALTKTMRDLHEGGRLISRVEEAKLERILSQHDPLVP"
     gene            9031..10158
                     /locus_tag="VLR27_10255"
     CDS             9031..10158
                     /locus_tag="VLR27_10255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP03874.1"
                     /translation="MAAAPTTEIGEAGALDSGLDAAVRAFRTAAWAWLTVVTVLSFAS
                     IERPVAAAVGVLAAGVVTVVWWLPSSPGRTDEVRQRLVAAELLVGAGLVAADGWVYDD
                     GRAQSFGGVWPLAGVLAVAVRSGRRAGVSAGLVLGAARAVGEVVVVRGEWSATQALAV
                     ASTGVLFGLAGWAAGWAAGRIRIAERVAVRSAARAEVAAELHDGVLQTLAVVQRRSDD
                     PDLVRLARSQEAELRRFLAGPDETPGGDVPVEVLLRAAAADAIARFGLRVEVAAVPPL
                     PAVPATHGAALRGVVGECLANVAKHAGTDRAVVFAEADDRCLHVSVTDEGAGFDPAAV
                     HERGIADSVRRRGEGLGGTTAIDARPGAGTTITVEIPLEER"
     gene            10155..10790
                     /locus_tag="VLR27_10260"
     CDS             10155..10790
                     /locus_tag="VLR27_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP03875.1"
                     /translation="MSSKIKVLVADDHAVWRSGMRAELGEAFWVVGEAEDAPTAIEMA
                     HRLHPDVVAADLRMPDGGGLRVAAELSDQFPVVILTVSEAHDDLLAAIGAGAVGYLVK
                     SSTVAEIGAGLAAASRGEPVFSASLAALVLGDFARPATTPDGRPVKALSAREREVLRL
                     VARGSTYRAVGEELFIAERTVENHVRNILEKLRLNRRDELIRWAAKRGLDE"
     gene            complement(10794..>11273)
                     /locus_tag="VLR27_10265"
     CDS             complement(10794..>11273)
                     /locus_tag="VLR27_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSP03876.1"
                     /translation="TNPYMGRPIEIDLPRYDKTWEVNLRGPLVWAQECWKASMQDNGG
                     AVINIASIGGMSVEPGIGIYNTTKAALIHLTKVLAADLSPGVRVNAIAPGLVKTDMAK
                     ALWETGEEQIAKRTPLHRLGEPEDIANAALFLASELSSWMTGHTMVVDGGALIRPTG"
BASE COUNT         1579 a         4002 c         4205 g         1487 t
ORIGIN      
        1 cggaccggtc ggctgggtcg acgccgccgg caacggcacg tgggccgtcg gcatccgatc
       61 cgccatcgtc gacggcaccg tggccaggct cttcgccggc gtcggcgtcg tcgccgactc
      121 cgacccgcag gccgagctgg ccgagaccga ggcaaagctg caggcggtgc tgtccgccat
      181 cgtccggccc tgagcgctcc cgggtcaggg caccacgtgt gatgcggggg tcgcggtcgt
      241 tcggccgttg gcggacgcgg tgcgtgcact tccgagcggc gagctgcgga cgacctgcac
      301 cgtggtgtcg cccacctgca gcaccgcgcc gggcatgagc acgacggccg aggagatccg
      361 gttgccgtcg aggtgcgtgc cgttcgtgct ccccaggtcc tccaccacga tggtctcacc
      421 tcggggcgcg aggttcgcat gtcgtcggga tgcctgaccg tcggcgatca ggacgccgtc
      481 gcactcccgg ccgatctcga ggggttcacg cagctccacg aacagcggac gacggcccgg
      541 ttgcgcgatc tcgaggacgg gatggctcac ggcgcggtgc cgatcagccg tagctcttgg
      601 tgtcgaggtc ccgcacggtg gccgccaggt gctgcagcag cttcttggac aaggacggca
      661 cgtcctggag cagcgcggag aactcacggg gaccgatgac caggacctcg gccgggccgt
      721 cgacgacgac cgtggcggtg cgcacgccgt ggtcgagcaa acccagctcg ccgaccgaat
      781 cgcccggacc gagctcggcg accttgcggc cgttgcgctt cacgaccgcc ttgcccgaga
      841 ggatgacgaa ggcctcgcgc gaggactcat cctgggtggt cagcgcatgg ccgtcgccga
      901 cgctcagctg gtcggaggac ttggcgatgg ccgccagctc cttcttcgag cacgccgaga
      961 acaacgggac gttggacagg tgatcaaccg ccgtgttctt tgacacagcg gaatcctatg
     1021 accgcagtca ctgctcgtgc cggaagttcg gtccacggcg gggacgagat ctcaggcgtg
     1081 cacggcgcgc acggcggctt ccgccagacg accgtgcagc gtgacgttct cctcccgatc
     1141 cgtgggaacc acgaggacgc gcacgcccgg tcgccactcg agctgctcga ccgagacgac
     1201 ccggtcagcg tcgacgccgt acgcccgggc gagcgccacc aggtcgagcc cgtgaggcgt
     1261 cccgaagagt cgctcgaagc ggtcggccgc cagctcgtga cgttgcggca ggaacgagaa
     1321 gatcccaccg ccgtcgttgt cgagcaccac gatcgtgaga tccacgtcac ggtctgccag
     1381 cccgagcaac ccgttggagt cgtgcaggaa cgccacgtcg ccgatcagca gtgtggtctt
     1441 ggctcccacc agcgccgcgc cgacagcggt ggagaccacc ccgtcgatgc cgttcgctcc
     1501 gcggttggcg aggacccgga ggtcgcgacg ggcgatgccg aaccactcga cgtcgcggac
     1561 cggcatcgac gacgaggtga cgaggacggc gccgggcgga agggtccgcg tgaggtgccg
     1621 ggcgacggcc ggctcggaca gcggggcgtc ggccagcacc tcgtcgatcg ccgtgccgac
     1681 ggcggcggcc gcgacccgcc aggcctcgac ccactcctcc acaggctcgt ggacgtcggg
     1741 gacgaccggg accgcgtcga ggaggaccgt ggcggtgtgg gagggatcgg gccaccacgc
     1801 gggggcggcg atcacgaggt cctcgtgatc gcagccgtcc agccactgcg cgaggacctt
     1861 ggacgcgggc atgcccccgg aacggatcac cacgtcgggg cgagcgcgct cggcgaacgc
     1921 cgtgcgcagc agcgggtcga agcactcgat gctcgtcgtg ctgctgcacc cggaggacgg
     1981 gtcggccagc aggggccagc cgaggtcttc ggcgagcgcc gagacggcct ccgggtcggc
     2041 ggttccccgt ccggcgatca ccacgccacg ggcggcgcac acggtcggat cgagcacggg
     2101 ggcgactcgc ccggcggcga ggcgctggtg ccacgggccg tctccccgac ccggcgggag
     2161 gtcgccgacc tcaccgagca acggttcgcg gaagggcagg ttcagctgca cgggcccggg
     2221 ccactccgcc gtggcttcgg cccaggtccg ggccgcgagg gagcgccagg tggaggcctg
     2281 cgtcggatca gccacgcccg ggtcggtgaa gaagcggacc gaccggccga acaggtgggt
     2341 ctgatcgacc gtctgcgggg cggcgacgtc gcgcagctcg ggtgggcggt cggcggtgca
     2401 caggatcagg ggcacgccgg cgaggtcggc ctcgaccgca gcggcgtgca gctggaccac
     2461 cgccgtcccg ctcgtcgtga ccacgaccac cggccggccc gctgtcgcgg ccaggccgag
     2521 ggcgaggaag gaccccgacc gctcgtcgtg gtgcacgtgg gtccgcaccc ggtcgtcgga
     2581 ggcgagcgcc acggtgagcg gcgtcgaccg cgaccccggg cagaccacgg catcggtgag
     2641 cccgcagcgc acccactcgt cgaccagcgt ggcgcagaag gtggcctgga cgtcgggact
     2701 ccagccgcgg gtgtcgttca ccgcgggtat gtcaccatgc ccggccatgt ggttgcacgc
     2761 cgagcgcgag ggatccggcc cgccactggt gctggtgcac ggcttcaccc agacccgtcg
     2821 ctgctgggga cctgttgccg aggacctggc gaccgaccac accgtcgtgc gggtcgacgc
     2881 ccccggacac ggcatgtccg ccgacatcat ggccggtctg cggggcggcg gccgcctcat
     2941 cgccgaccag gggggcgagg ccacgtacct cggctactcg atgggcggcc gctacctcat
     3001 ccacctcgcg ctggcgaacc ccgagctcgt ccgtggcctc gtgctcctcg gagcgacggc
     3061 cggcatcgac gaccccgacg accgggccgc acggctgcgc gccgacgagg ccaccgcggc
     3121 ccggatccgc gagctcgggc tgctcgagtt cctgcagacc tggatcgcgc agccgatgtt
     3181 cgccggcatc cccgccgagc gccagtacct gctcgaacgg atggagaaca ccgtcgacgg
     3241 cctcgagagc tccctgttgc agtcgggcac cggcgcccag gacccctcct gggacaagct
     3301 gcaccgcctc gacatgcccg tgctcgtggt cgccggggag cgtgacgaca agttcgctgc
     3361 actcggtcag cggttgcggg gatcgatcgg tgacaacgcc accttcgcca cggtgcccgg
     3421 cgccggacat gccgcgcacc tggagcagcc cgaggccttc ctcgacctgt tgcgtccctg
     3481 gctcgccgcc cacgacctgt agcgcgccgc ggggcacgcc tctgaggcgt gccccgcgtc
     3541 ccggaacggg atggcgacgg ctacaggcca gcggcgacgg ctgcgtcgat gacgtcctgg
     3601 ggcacggtgc acgtcgacgc cgactcggcc accagcccga acagggtgtc ctggtcggag
     3661 ccggccatgg cgaggagctc ggactccggc gtgcccgcca gcagctgctc gtgcgtgcag
     3721 ttggcggcac gggggtcggc cccctgggcg gtgagcaggt tgaacacggt ggactggacc
     3781 agcccctcgg gggcctcctc ggggtagtcg tcgagggaga gctccccggt cggttccggc
     3841 gccgcagtcg tggtggtggt cgtgacgggc agggcgacgg gcgtgtaggc gatctccacc
     3901 gagtctccgg cgtccctctc ggccgacgcc cacagcccca cggcgatggc agcgaccacg
     3961 aggacgccca ccacccaggc gatgagacgc gggccctgag gttgcgcggc gagccggacg
     4021 gcagcgccgg gcgcgtgccc ctcgaccggg aagtagccac gctcgtcggc ccacatcagc
     4081 agcgggggca tcacgaccag ggcagcgagg agcgccaccg tcacgttcag cgtgacgatg
     4141 atgccgaagt cccgcaggag gggcagggcc gatccgatca gcacggcgaa cccaccgacc
     4201 gtggtcgcag ccgaggtgaa gaacgctcgc ccggtgcgtc gggccgcgtg gtccgacgcc
     4261 tccttcggcg agcgtcccga ctggcgctcc tccaggtacc gggcggtgat cagcacggag
     4321 aactccgtgc aggacgcgat gacgagcggc ccggacacgg tggtgagcgg gctcagcgtg
     4381 agcccgaagg cgccgatgac gaccgatgac gtgccgatgg cgaggccgac gggcacgagc
     4441 gtgagggcgg cacggcccag cgactgcagc cggatcagga ggaacagcgc ggcgagggcg
     4501 agcccgaggt aggtgagcac ggcccggttc gccgacaggt tctcgagcag accgacgccg
     4561 accacggcca ggccggccgg cgtggcccgc atcgcgggct cgtccggccc gaggtcctcc
     4621 agcaggatcg agtcctcggg caggtcgagc gcagcgatcc gctgatcgag gtcggcctcg
     4681 agcacctcga ccagatcggc ccggtcctcc aggctgcccg gcgccaggcg caggttcacc
     4741 tgcgtcgcgg tcccgtcgtc ggacagcagg accttgcgga tgtcctcggg catgaccgcc
     4801 gcggcggccg cagcgtcggc cgaggtcggc gcgatctcgg tcgcgcccgg gatgttgatg
     4861 atcttggcca tcgtcccggt gagcgaggag gaggtggcca cctcatcgag gccctcggcg
     4921 taggtggtga agtcgtggag cacctgggtc aggtcctcgt cgaggacgtt gttggcgctg
     4981 accaggacgc tgagcgtcga ggagaacccg gtggcctcct cgagggtgtc gaggtcgcgc
     5041 acggtctggg tgtcctggtt gatccagcgg accgggtcgg actcgatctc gaagcgaccc
     5101 tccatggcga cgccgccgac gaacagcagt gccgacagga cgacgagggg caccgcggcc
     5161 ttgggcggga ggccaccgag ccacacgacg aagcgctcga cgcgggactc ggccacgtcc
     5221 cccgtgcggg accggtactc gcggacgccg aggatggtgg tgggcaggac gatgccgcag
     5281 aagacgaggg cgacgatgcc gacggccagc atgacgccga agtcccggat catcggcacc
     5341 tggctgagct ggagggccat gaaggccacg accgcggcga gcgtggcggc gacgagcgcc
     5401 ggtccgacgt tggcgagggt ctcgctgatc gggtgctcgt ccttgtcgag tgcagcctcc
     5461 tcctcgatgc ggttgtgcac ctggatggcg aagtcgatgc cgagcccgat gaggatcggc
     5521 aggccgctga tcgtcaccag cgacaggtcg atgccgatgt agccgaggag ggcgaacccc
     5581 cacaagacgc cgacgacggt ggagagcagc ggcaggaagc gccagcgcac ccggaacatc
     5641 acgagcagga tcacggccat gatcgccagg gcgatggcgc cgagggtgag catgccgccc
     5701 tgcaggtagt cgttgatgtc cttgaggaac accggtgagc cggtgaccac gacctcgaag
     5761 ccgtcgagct gggcgctctc ggcgacgtcg aggacggcct gggtgcccga cgacagcgtg
     5821 tcgaggtcgg cgttgccctg gaggaggacg ccgccgacgg cggtctggag gttggggaag
     5881 gtgctctgca gcgagaggcg caccacccgg tcgtcgccgg tgggcatctc cgggaccccg
     5941 tcgaccacct cgacgttctg gttgccgaac agcaggaact cgatccagcc cgggttcgac
     6001 aggtcccgct cctccaccgc gccgaggcgg gcgagcgtcg tggtgacgtc ggcctggcgg
     6061 atggcctggg actcctcgtc cggctcgcgc tcgagggcgc tcagcagcgc gccggtgccg
     6121 ggcgacggtt cgatcgtctc gcccgagggg gccacgccgg tggtgatcga ctcgctgaac
     6181 tgcacggtcg tgagcggcgc gacgacggag tacacctcgt cgatggcccg cagctcggac
     6241 tcgatgcgct cgagctcctc gaggttgccc tcggcgtaca ggtcggagat gtcggcgtca
     6301 ccggtcgcct ggaacagcag gatgatcgtc tcaccgccga actggctctg gagtgcctcg
     6361 ttgtcgatgg cgatctggtc gtcgtcgtcg aggtagctgt cctggccggt ggcgaactcg
     6421 aggttcgtcg cgccgagggc gaggatcgcc gtgaccacca ggaacgagat gatcacgatc
     6481 acccatcgtc gtccgagctc gaaccccaac ctgcgccaaa gacgttccac ggcggggcat
     6541 gctacgacgt cccctcctcc cggacagcag aggacaaggt cccgtcgtcg cgcttcaggc
     6601 ggagatgacc agccccaggg cgaggagcag tccgaagacc agctgcacct gcccggtcgc
     6661 gccgagcacc gcgatcaggt cgcgccccgc tgcgccggac cgcaccaggc ggatcggacg
     6721 gagggcgggg acggccgcga gcagcgccac gatcgcggcg ggccgggcga tcgcgaccgg
     6781 cacgaccagg agcagcgcga ccacgagcat ggccacgaac agggtgcggg tgcgcgcgtc
     6841 gccgaggcgc acggccaggg tctgcttgcc ggcgaccgtg tcgcccggga tgtcacgcag
     6901 gttgttggtg acgagcagcg cggtggcgag caggcccacc ggcaccgacg ccagcaggct
     6961 gaggccggtg acctcctcga cctgcacgta ggtggagccg acggtggcga ccacgccgaa
     7021 gaacacgaag acgaagacct cgccgagccc caggtagccg tagggccgtg agccaccggt
     7081 gtagaaccag ccggcgagca tgctggccgc gccgaccagc agcagctccc agccgaccgc
     7141 gagggtgagg gcgagcccgg ctgctcccgc cacggcgaag gacgccatcg ccgcgcgctt
     7201 gaccgccgag ggctccttga ggccccaccc gacgaggcgg acgggaccga cccggccgcc
     7261 cgggtcgtcg gtgccgcgga tgccgtcgct gtagtcgttg gcgtagttgg tggccacctg
     7321 gagggcgagg gcgaccacgg cggcggcgac ggcgcgccag gcgatgagcg gtccgtcgac
     7381 cccccgggcg gcggcggtgc cgaccagcac ggggacgacc gcggcgggca gcgtccggac
     7441 ccgggcaccg gcgatccagg cctgcagtcc ggtgggggtc ggtgccgtca tccggagcag
     7501 gttcgcgcac cgagggtcct accctgcacc cggtgcagag gctggtggcg atcgaggcgc
     7561 ggggcgagcg gttcaccgac gccctgcgcg ccgcctggga cgccggcgac gccgtgctcc
     7621 ccgtcgatcc gaggttgccc gcccgaacgg cctccgcgct cgtcgacgcg ctgggcggag
     7681 gcgggccgat cgacgccgag gtggcgctcg tcgtgccgac cagcggcacc accggcgacc
     7741 ccaagggcgt ggagctgacc cacgcgcagg tggcggcgtc ggccgaggcc acctcgtcct
     7801 tcctcggtgt cgaacccgcc cggcaccgct ggctggcgtg cctcccgctg gcccacgtcg
     7861 gcggcctgtc cgtggtgacc cgcgcctggc acaccggcac cccgctcgag gtccacgacg
     7921 gcttcgacgc cgccgcggtc gacgcggctg cccgcgccgg gtgcacgcac gtctccctcg
     7981 tgggcaccgc gctggcccgc atcgacgcat cggcgtggac ccggatcgtc ctggggggtt
     8041 ccgcaccccc gccggaccgg ccggcgaaca ccgtcgccac ctacggcatg accgagacgg
     8101 gcagcggcgt cgtctacgac ggcgtcgcgc tgccgggagt ggagctgcgc atcgtcgacg
     8161 gcgagatcct ggtgcgcggg ccgatggtcg ccacccgata ccgggacggg accccggtgg
     8221 tggacgccga cggctggctg cacaccggag acgccggccg cctcgacgac ggcgtcctca
     8281 ccgtgcacgg ccggcggggc gacatgatca tcaccggcgg cgagaacgtg caccccgacc
     8341 cggtggagca gcgcctccgc gagcaccccg ccgtcgccga cgccgccgtg gtcgggcgtc
     8401 ccgacccgga gtggggccag cgggtcgtgg ccgtgctcga gctcgcaccc gaggccaccg
     8461 tcccctcgct cgccgagctg cgcgactggg tccgcgacgc cctcccgccg tggacggcac
     8521 cgaaggagct cgaggtcgtc gacgcgctgc cgcggacgtc gctcggcaag gtgcggcgcg
     8581 ccgccgtccg ctgaacgcca tgggggatgg cccccatcgt cgacgcggga tccccccggt
     8641 gggcggggtc cgggagcatt cgtacggtcg tcgcagtggc catccgggcc gcatccacga
     8701 ccaggaggaa ctcccatgag cacctacgaa ccccagccca gccagctcca ctccccggcc
     8761 tgggtcgccg tcaccaagat ccagttcgcc gtctccgtcc tcgccctcgt gatcggcctg
     8821 gcgtacctgc cggtcgagcc gtggatcgtc gcctacctgg tgatgggcac cctgctcctc
     8881 acctcctcga cggtggcgct gaccaagacg atgcgggacc tccacgaggg cggccggctg
     8941 atctcccggg tcgaggaggc caagctcgag cgcatcctgt cccagcacga tccgctggtg
     9001 ccgtgaggcc ggtcgggcaa gatctgtccc gtggctgctg ccccgacgac cgagatcggc
     9061 gaggccggcg cactcgactc cgggctcgac gccgccgtgc gggccttccg cacggcggcg
     9121 tgggcgtggc tgacggtcgt caccgtgctg tcgttcgcct cgatcgagcg gccggtggca
     9181 gcggcagtcg gcgtcctcgc cgccggggtc gtcaccgtcg tctggtggct gccgtcgtcg
     9241 cccgggcgca cggacgaggt ccggcagcgc ctggtcgccg ccgagctgct ggtcggtgcc
     9301 ggtctcgtgg ccgctgacgg ctgggtctac gacgacgggc gggcccagag cttcggcggg
     9361 gtctggcccc tcgccggcgt cctcgccgtg gcggtccgct ccgggcgccg ggcaggcgtg
     9421 tcggccgggc tggtgctcgg cgcggctcga gcggtcggtg aggtcgtggt cgtccgtggc
     9481 gagtggtcgg ccacccaggc gctggccgtg gcctccaccg gtgtcctgtt cggcctcgcc
     9541 ggatgggctg ccgggtgggc ggccgggcgg atccgcatcg ccgagcgggt ggcggtgcgc
     9601 agcgcggccc gggccgaggt cgccgccgag ctgcacgacg gcgtgctcca gaccctcgcc
     9661 gtcgtccaac ggcgcagcga cgaccccgac ctcgtccggc tggcccgctc ccaggaggcc
     9721 gagctgcgcc ggttcctggc cggtcccgac gagaccccgg gcggcgacgt cccggtcgag
     9781 gtcctgctcc gggccgctgc cgccgacgcc atcgcccgct tcgggctccg cgtcgaggtg
     9841 gcggcggtgc cgccgctgcc ggccgtgccc gcgacgcacg gggcggcgct ccggggtgtc
     9901 gtcggcgagt gcctggccaa cgtggccaag cacgccggca ccgaccgggc cgtcgtgttc
     9961 gccgaggcgg acgaccgctg cctgcacgtg tcggtcaccg acgagggcgc cgggttcgac
    10021 cccgccgccg tccacgagcg gggcatcgcc gacagcgtcc gccgccgcgg cgaggggctg
    10081 ggcggcacga ccgccatcga cgcccgtccg ggtgccggca ccaccatcac cgtggagatc
    10141 cccctggagg agcgatgagc tcgaagatca aggtgctcgt cgccgacgac cacgccgtct
    10201 ggcgctcggg gatgcgggcc gagctcggcg aggcgttctg ggtggtcggc gaggccgaag
    10261 acgccccgac ggcgatcgag atggcgcacc gcctgcaccc cgatgtcgtc gccgccgacc
    10321 tgcgcatgcc cgacgggggt gggctgcggg tggcggccga gctgtccgac cagttccccg
    10381 tcgtgatcct caccgtgtcc gaggcccacg acgacctgct ggccgccatc ggggccggcg
    10441 ccgtcggcta cctggtcaag tcctcgacgg tggccgagat cggcgccggc ctggccgccg
    10501 cctcccgggg cgagcccgtg ttctccgcca gcctggccgc cctcgtcctc ggcgacttcg
    10561 cccggccggc caccaccccg gacggtcgcc cggtgaaggc gctgtcggcg cgggagcgcg
    10621 aggtgctgcg gctggtggcc cgcggcagca cctaccgcgc cgtgggcgag gagctgttca
    10681 tcgcggagcg gaccgtggag aaccacgtcc gcaacatcct ggagaagctg cgcctcaacc
    10741 ggcgcgacga gctgatccgc tgggcggcca agcgcggcct ggacgagtga tcgctacccc
    10801 gtcggacgga tgagggcgcc gccgtcgacg accatcgtgt gaccggtcat ccacgacgac
    10861 agctccgagg cgaggaacag ggcggcgttg gcgatgtcct cgggctcgcc gaggcggtgc
    10921 agcggggtgc gcttggcgat ctgctcctcg ccggtctccc acagggcctt ggccatgtcg
    10981 gtcttcacca gcccgggggc gatggcgttg acccgcacgc ccggtgacag gtcggcggcc
    11041 agcaccttgg tgaggtggat cagcgcggcc ttggtcgtgt tgtagatgcc gatcccgggc
    11101 tcgaccgaca tgccgccgat cgaggcgatg ttgatgacgg cgccgccgtt gtcctgcatg
    11161 gacgccttcc agcactcctg ggcccacacg agcgggccgc gcaggttgac ctcccaggtc
    11221 ttgtcgtagc ggggcaggtc gatctcgatc gggcggccca tgtacgggtt cgt
//
LOCUS       DATISU010000257         2522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_759768, whole genome shotgun sequence.
ACCESSION   DATISU010000257 DATISU010000000
VERSION     DATISU010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759768"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="VLR27_10270"
     CDS             <1..1287
                     /locus_tag="VLR27_10270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HSP03877.1"
                     /translation="DDHVNPPKATDEIWVGDSRDMDTYGDIADGSVALVVTSPPYFAG
                     KAYEEALGTDGIPADYLEYLGMLRDVFAECVRKLEPGGRIAVNVANLGRKPYRSLSSD
                     VVAIFEELGLLLRGEIIWQKGKGAGGSCAWGSFQRPGNPVLRDVTERVIVASKGRFDR
                     AVDAKDRASAGMPSTGTMTVDDFLEATLDVWELAPESAKRVNHPAPFPVELPHRLIDL
                     YTYAGDLVLDPFMGAGSTAVAAARSGRHFVGFDTDPDYVEAALARVEAERGDGPPVEV
                     RPGKGTLPDDAPAAEAIRVGRKATDVAKRLLVEAGFTDVVDKKAPVRELGVEFDLAAT
                     DTAGRRWLVTVAGSFTPAPSGLRRSDVLWRTLAQAAVVHAAGRPEGVLVLTTEQPARS
                     TAQGKALAAVIGRGIDAVVELTAEGAVDELRSLHPA"
     gene            1295..2302
                     /locus_tag="VLR27_10275"
     CDS             1295..2302
                     /locus_tag="VLR27_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03878.1"
                     /translation="MADTRTAVSELATALGMLVHGTVGWAVLQRPAEVLVGPDAWDQL
                     EHGLHDPDLHVLAEAAFANGTAFAEHAEGLDGRRPRRIEWTGGHRPPGDQVIPADLRV
                     DRVYLVSCKYLSKVLHNAAPARVFDHLLKSTDSHDRADWYGQVAPDAHRRLATVAAEA
                     VGCPFPADPCDADVTERRRLRDALREHGRAWPDAATEPYREMCAEVSRASAERWRRSA
                     STSGAREQLLWRLLRLASAPYFVLGASRVDVIRLRIDTPWDWRQRHDLRSFDIEPDDA
                     GQPQVRWAATVVDRESGTDVVVHGHVEVRWSHGRFGGPPEAKVYLDTPPAEVPGYVPL
                     T"
BASE COUNT          319 a         1009 c          877 g          317 t
ORIGIN      
        1 gacgaccacg tcaacccgcc caaggccacc gacgagatct gggtcggcga ctcgcgcgac
       61 atggacacct acggcgacat cgccgacggc tccgtcgccc tggtcgtcac ctcgccgccc
      121 tacttcgccg gcaaggccta cgaggaggcc ctcggcaccg acggcatccc ggccgactac
      181 ctcgagtacc tcggcatgct gcgcgacgtc ttcgccgagt gcgtccgcaa gctcgagccg
      241 ggcggtcgca tcgcagtcaa cgtcgccaac ctcggccgca agccctaccg gtcgctgtcg
      301 tccgacgtgg tcgccatctt cgaggagctc ggcctgctcc tgcggggcga gatcatctgg
      361 cagaagggca agggcgccgg cggctcctgc gcctggggca gcttccagcg accgggcaac
      421 ccggtgctgc gcgacgtcac cgagcgggtc atcgtcgcct ccaagggccg cttcgaccgg
      481 gccgtcgacg ccaaggaccg ggcgtccgcg ggcatgccca gcaccggcac catgaccgtc
      541 gacgacttcc tcgaggccac cctcgacgtg tgggagctgg ccccggagag cgccaagcgg
      601 gtgaaccacc cggccccgtt cccggtcgag ctgccccacc ggctcatcga cctctacacc
      661 tacgccggcg acctcgtgct cgacccgttc atgggcgccg gctccaccgc cgtcgccgcc
      721 gcccgctccg gccgccactt cgtcggcttc gacaccgacc ccgactacgt cgaggccgcc
      781 ctcgcccgcg tcgaggccga gcggggcgac ggcccgccgg tcgaggtgcg cccgggcaag
      841 ggcaccctgc ccgacgatgc gccggccgcc gaggccatcc gcgtcggccg caaggccacc
      901 gacgtggcga agcgcctcct ggtggaggcc ggcttcaccg atgtcgtcga caagaaggcc
      961 ccggtccgcg agctcggcgt cgagttcgac ctggccgcca ccgacaccgc cggtcgccgc
     1021 tggctggtga ccgtcgccgg ctccttcacg cccgccccgt ccggcctgcg ccggtccgac
     1081 gtgctgtggc gcacgctggc ccaggctgcg gtggtccacg ccgccggtcg ccccgagggc
     1141 gtgctggtcc tcaccaccga gcagccggcc cggtccaccg cgcagggcaa ggcgctggct
     1201 gcggtcatcg gccggggcat cgacgcggtc gtcgagctca ccgccgaggg cgcggtcgac
     1261 gagctgcggt ccctccaccc ggcctgacgc cccggtggcc gacacccgga cagcggtctc
     1321 cgagctcgcc accgcgctcg gcatgctcgt ccacggcacc gtgggctggg ccgtgctgca
     1381 gcgacccgcc gaggtgctcg tcggccccga cgcctgggac cagctcgagc acggcttgca
     1441 cgaccccgac ctccacgtgc tggccgaggc cgcgttcgcc aacggcaccg cgttcgccga
     1501 gcacgccgag ggcctcgacg gccgccgccc gcgtcgcatc gagtggaccg gcgggcaccg
     1561 cccgcccggc gaccaggtca tccccgccga cctgcgggtc gaccgggtct acctggtgtc
     1621 gtgcaagtac ctctcgaagg tcctgcacaa cgcagcgccg gcgcgggtgt tcgaccacct
     1681 gctgaagtcc accgacagcc acgaccgcgc cgactggtac ggccaggtgg cgccggacgc
     1741 acaccgccgg ctggcgacgg tcgccgccga ggcggtgggc tgcccgttcc cggccgaccc
     1801 ctgcgacgcc gacgtgaccg agcgccgccg cctgcgcgac gccctccgcg agcacgggcg
     1861 cgcctggccc gatgcggcga cggagccgta ccgggaaatg tgcgccgagg tgagccgggc
     1921 ctcggccgag cgctggcgcc ggtcggcgtc gacctccggt gcccgggagc agctgctgtg
     1981 gcgcctgctc cggctggcgt cggcgcccta cttcgtgctc ggcgcgtccc gcgtcgacgt
     2041 catccgcctg cggatcgaca cgccgtggga ctggcgccag cgccacgacc tgcggtcctt
     2101 cgacatcgag cccgacgacg ccggccagcc ccaggtgcgc tgggcggcca cggtggtcga
     2161 ccgggagtcc ggcacggacg tggtcgtcca cggccacgtc gaggtgcgct ggagccacgg
     2221 ccgctttggg ggcccgcccg aggccaaggt gtacctcgac accccaccgg ccgaggtgcc
     2281 cggctacgtg ccgctcacct gagcggcagc gtgttccgcg gccgctgcca gttccccgcc
     2341 gaggccactc ggagcgggtg tcagggcagg ccggcgcccg ccacggcgac gtcgatggcc
     2401 tcgatgcgcc cggcccgggc ctcccgagca cccaccggct cgctgtcgat ggccgtgcag
     2461 acgtagagcg tgcgccggtc gtcgccgccg agcatgcagg cgaagggctg acgctcgtcg
     2521 ct
//
LOCUS       DATISU010000258         5079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_247325, whole genome shotgun sequence.
ACCESSION   DATISU010000258 DATISU010000000
VERSION     DATISU010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247325"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VLR27_10280"
     CDS             <1..1086
                     /locus_tag="VLR27_10280"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HSP03879.1"
                     /translation="GMFTANTMASISEALGMSLPGSASAPAVDGRRDDLAYESGKAVM
                     ELIRKGIRPRQIMTRKAFENAIAVTMALGGSTNAVLHILAIAYEAEVELDLAVFNEIA
                     AKVPHIADTKPHGKYHMVDIDRVGGVPVVMKHLLDAGLLHGDCLTVTGKTVAENLAEL
                     DPPAPDGEVIHPLSDPIHDIGGLAILTGTLAPKGSVVKVAGIDFDEFEGPARVFDGEQ
                     DAMDAVMAGRINSGDVVVIRYEGPKGGPGMREMLAITGAMKGAGRGGDAALITDGRFS
                     GGTHGFCIGHVAPEAVDGGPIGLVEEGDRIRIDVRGHTIDLLVDDAVLEERRKALKHP
                     EPRYTKGVLAKYARLAAGAEKGAVTEA"
     gene            1156..1656
                     /gene="ectA"
                     /locus_tag="VLR27_10285"
     CDS             1156..1656
                     /gene="ectA"
                     /locus_tag="VLR27_10285"
                     /EC_number="2.3.1.178"
                     /inference="COORDINATES: protein motif:HMM:TIGR02406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="HSP03880.1"
                     /translation="MAPPSASAQDKALEYRFGRLEDGPALWSLAKANGLDENSPYAYL
                     LWTEYFRDTTVVATDDADAPIGFVTAFLRPDDASTVFVWQIGVDDAHRRRGIAGRLLD
                     ELFERTGATALEATVTPSNSASETLFRRFGDRHGLSVAVEPLFTEELFPPGHEAELRF
                     RIAPRD"
     gene            1714..3009
                     /gene="ectB"
                     /locus_tag="VLR27_10290"
     CDS             1714..3009
                     /gene="ectB"
                     /locus_tag="VLR27_10290"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: protein motif:HMM:NF006733.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HSP03881.1"
                     /translation="MSIFERLESEVRGYCRSWPAVFKSAHGHTMVAEDGTEYLDFFAG
                     AGALNYGHNHPVLIEALQKHLAEQGFIHALDISTTTKRTFMERFEDIILKPRNLDYKV
                     QFPGPTGTNAVEAALKLARKATGRHRVVGFTNGFHGMTLGSLAVTGNAMKRGGAGVPL
                     GDSTSMPYANYLSDDMDSLHILEAMISGGSSGLDEPAAVIVETTQGEGGINPASHQWM
                     RNLDDICKRHGILLIVDDIQVGNGRTGPYFSFEEMGIDPDIVTLSKSISGSGLPMALV
                     LMKPEHDVWAPGEHNGTFRGNNAAFVTATAALEHFWTDDELTKSTAAKSDKVRAGLES
                     IMEGHPGLFTDVRGRGLILGIETAVPEIAGTIAKGAYERGLIIETAGAEDEVVKLLPP
                     LIVTDEAIDQALEILNEAADTISDDMVAELRAEREEVRS"
     gene            3006..3413
                     /locus_tag="VLR27_10295"
     CDS             3006..3413
                     /locus_tag="VLR27_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine synthase"
                     /protein_id="HSP03882.1"
                     /translation="MIVRTLQDIVGTDRDVDGGNWSSRRLSLAEDGMGFSMHDTILRA
                     GTETHMWYTNHLEGVYCIEGKATLEDKATGEVHEITPGTIYLLNDNDRHVLRVEEDLR
                     VICTFNPPVTGQEVHDEDGAYPLLTAEDQPEPA"
     gene            3410..4315
                     /gene="thpD"
                     /locus_tag="VLR27_10300"
     CDS             3410..4315
                     /gene="thpD"
                     /locus_tag="VLR27_10300"
                     /EC_number="1.14.11.55"
                     /inference="COORDINATES: protein motif:HMM:TIGR02408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine hydroxylase"
                     /protein_id="HSP03883.1"
                     /translation="MSTTHTDAPTHTGDPYPSRMGRAPELLPRVDPVVHGPATDGPLD
                     MGQVAAFEQNGYLTVPGVFSADEVRGLDQLLSDLVNGGDIDSDQVIREPDSAVIRSVF
                     AFHEGDGGLADVPSDPRLAGVARQLLGSDVYIHQSRVNRKPGFRGKDFQWHSDFETWH
                     TEDGMPRMRCLSAVVALTDNEPWNGSLLVMPGSHKWFVTCPEPTPPANHEHSLKVQEV
                     GVPDEDSLTTLFDRHGIDQCTGKAGSVLFFDCNLMHGSSTNISPLPRRNLFMVFNSVE
                     NTIGDPYSAPQRRPEHIASRTFTPV"
     gene            4453..>5079
                     /locus_tag="VLR27_10305"
     CDS             4453..>5079
                     /locus_tag="VLR27_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HSP03884.1"
                     /translation="MQQLSGVDASFLYMESPTTFGHVSSLIVYDPKDSDSAGYEATRA
                     LFQERLHLLDPMRRRLVHVPFDLDHPYWVEDPELDLDFHIRQTAVAPPGTDEQLSELV
                     SRIIARPLDRSRPLWELYVIYGLSDGRIAQLTKIHHAAIDGVGGAQLLANILDLEPDA
                     PPRPGPCPDPERIPTQEEMLVRGLTSLATRPYKQGRLAVRMLRELPQIG"
BASE COUNT          857 a         1960 c         1577 g          685 t
ORIGIN      
        1 gggatgttca cggccaacac catggccagc atctccgagg ccctcggcat gtcgctgccc
       61 ggcagcgcca gcgccccggc ggtcgacggt cgccgcgacg acctcgccta cgagagcggc
      121 aaggcggtca tggagctcat ccgcaagggc atccgccccc gccagatcat gacccgcaag
      181 gccttcgaga acgccatcgc cgtcaccatg gccctcggcg gctccaccaa cgcggtgctg
      241 cacatcctgg ccatcgccta cgaggccgag gtcgagctcg acctggcggt gttcaacgag
      301 atcgccgcca aggtccccca catcgccgac accaagccgc acggcaagta ccacatggtc
      361 gacatcgacc gcgtcggcgg cgtgccggtc gtgatgaagc acctgctcga cgccggcctc
      421 ctccacggcg actgcctcac cgtcaccggc aagaccgtgg ccgagaacct ggccgagctc
      481 gacccgcccg ctcccgacgg cgaggtcatc cacccgctgt cggaccccat ccacgacatc
      541 ggcggcctcg ccatcctcac cgggacgctg gcgccgaagg gctcggtcgt caaggtcgcc
      601 ggcatcgact tcgacgagtt cgagggcccg gcccgcgtgt tcgacggcga gcaggacgcc
      661 atggacgccg tgatggccgg tcggatcaac agcggcgacg tcgtcgtcat ccgctacgag
      721 gggcccaagg gcggcccggg catgcgggag atgttggcca tcaccggcgc catgaagggc
      781 gcgggtcgtg gcggcgacgc cgccctcatc accgacggcc gcttctcggg cggcacgcac
      841 gggttctgca tcggccacgt cgcccccgag gccgtggacg gcggccccat cgggctggtg
      901 gaggagggcg accgcatccg catcgacgtg cggggccaca ccatcgacct gctggtcgac
      961 gacgccgtgc tcgaggagcg ccgcaaggcc ctgaagcacc ccgaaccccg ctacaccaag
     1021 ggcgtgctgg ccaagtacgc ccgcctcgcc gccggcgccg agaagggcgc ggtcaccgag
     1081 gcctgagcgg catatgtgac ccatcccacg tcactttgtg ttctcggtca cagggcgact
     1141 aggtatgaaa ggaccgtggc ccctccgtct gccagcgcac aggacaaggc cctcgagtac
     1201 cggttcggcc ggctcgagga tggtccggcg ctctggtccc tcgcgaaggc caacggactc
     1261 gacgagaact cgccctacgc gtacctgctg tggaccgagt acttccggga caccaccgtc
     1321 gtcgccaccg acgacgctga tgccccgatc gggttcgtca ccgcgttcct gcggcccgac
     1381 gacgcctcca cggtgttcgt ctggcagatc ggcgtcgacg acgcccatcg ccgccggggg
     1441 atcgctggcc gtctgctcga cgagctgttc gagcgcacgg gtgcgaccgc actggaagcc
     1501 acggtgacgc cgagcaactc ggcgtccgag accctcttcc gtcgcttcgg cgatcgccac
     1561 ggcctgtccg tggcggtgga acccctcttc accgaggagc tgttcccccc gggtcacgag
     1621 gcggagctcc ggttccgcat cgccccgcgc gactgagggt cacgcggtga gccccgctcg
     1681 ccacgaccgt ccgtcaaccc cccaaaggga aaaatgagca tcttcgaacg actcgagtcc
     1741 gaggtccgcg gctactgccg ctcctggcct gccgtcttca agtccgccca cggccacacg
     1801 atggtggccg aggacggcac cgagtacctg gacttcttcg ccggggccgg cgccctgaac
     1861 tacgggcaca accaccccgt cctcatcgag gcgctgcaga agcacctcgc cgagcagggc
     1921 ttcatccacg ccctcgacat ctccaccacc accaagcgca cgttcatgga gcgcttcgag
     1981 gacatcatcc tcaagccgcg gaacctggac tacaaggtcc agttccccgg gcccaccggc
     2041 accaacgccg tcgaggccgc gctgaagctg gcccgcaagg ccaccggtcg ccaccgcgtc
     2101 gtgggcttca ccaacggctt ccacggcatg acgctgggtt cgctcgccgt caccggcaac
     2161 gccatgaagc gcggcggcgc cggcgtgccc ctcggtgact ccacgtccat gccctacgcc
     2221 aactacctca gcgacgacat ggacagcctc cacatcctcg aggccatgat ctcgggcggc
     2281 tccagcggcc tcgacgagcc cgccgccgtc atcgtggaga ccacgcaggg cgagggcggc
     2341 atcaacccgg cctcgcacca gtggatgcgc aacctcgacg acatctgcaa gcgccacggc
     2401 atcctgctca tcgtcgacga catccaggtc ggcaacggcc gcaccggccc ctacttctcc
     2461 ttcgaggaga tgggcatcga ccccgacatc gtcacgctgt cgaagtccat ctcgggctcc
     2521 ggcctgccga tggcgctcgt gctgatgaag cccgagcacg acgtgtgggc gccgggcgag
     2581 cacaacggca ccttccgcgg caacaacgcc gccttcgtca ccgccacggc ggcgctggag
     2641 cacttctgga ccgacgacga gctcaccaag tcgaccgcgg ccaagtccga caaggtccgc
     2701 gccgggctcg agtcgatcat ggagggccac cccggactgt tcaccgacgt ccgcggccgg
     2761 ggcctgatcc tcggcatcga gaccgccgtg cccgagatcg ccggcaccat cgccaagggc
     2821 gcctacgagc gcggcctcat catcgagacc gccggcgccg aggacgaggt cgtcaagctc
     2881 ctgcccccgc tcatcgtcac cgacgaggcc atcgaccagg ccctcgagat cctgaacgag
     2941 gctgccgaca ccatcagcga cgacatggtc gccgagctgc gcgccgagcg cgaggaggtc
     3001 cggtcgtgat cgtccgcacc ctgcaagaca tcgtcggcac cgaccgcgac gtcgacggcg
     3061 gcaactggtc gtcacgtcgc ctctcgctgg ccgaggacgg catgggcttc tccatgcacg
     3121 acacgatcct gcgagccggc accgagacgc acatgtggta cacgaaccac ctcgagggcg
     3181 tctactgcat cgagggcaag gcgaccctcg aggacaaggc cacgggcgag gtccacgaga
     3241 tcaccccggg caccatctac ctcctcaacg acaacgaccg ccacgtgctg cgggtcgagg
     3301 aggacctgcg cgtcatctgc acgttcaacc cacccgtcac cggccaggag gtccacgacg
     3361 aagacggcgc ctacccgctg ctgacggccg aagaccagcc ggagcctgca tgagcaccac
     3421 gcacaccgac gcacccaccc acaccggcga cccgtacccg tcccgcatgg ggcgcgcccc
     3481 ggagctgctg ccccgggtcg accccgtcgt ccacggtccc gccaccgacg gccccctcga
     3541 catgggacag gtcgccgcct tcgaacagaa cgggtacctc accgtgcccg gggtgttcag
     3601 cgccgatgag gtccgcggcc tcgaccagct gctcagcgac ctcgtcaacg gcggggacat
     3661 cgacagcgac caggtcatcc gcgagcccga ctcggccgtg atccgctcgg tcttcgcctt
     3721 ccacgagggc gacggcggcc tggccgacgt gccgtccgat ccccgcctgg ccggcgtcgc
     3781 ccgccagctc ctcgggtccg acgtctacat ccaccagagc cgcgtgaacc gcaagcccgg
     3841 gttccgcggc aaggacttcc agtggcactc ggacttcgag acctggcaca ccgaggacgg
     3901 catgccccgc atgcgatgcc tcagcgccgt ggtcgccctc accgacaacg agccgtggaa
     3961 cggttcgctg ctggtgatgc ccgggtccca caagtggttc gtgacctgcc cggagcccac
     4021 gccgcccgcc aaccacgagc actcgctcaa ggtccaggag gtcggcgtcc cggacgagga
     4081 cagcctcacg acgctgttcg accgccacgg catcgaccag tgcaccggca aggccggctc
     4141 cgtgctgttc ttcgactgca acctgatgca cgggtcctcg acgaacatct cgccgctgcc
     4201 ccgacgcaac ctgttcatgg tgttcaacag cgtcgagaac accatcggcg acccgtactc
     4261 ggccccgcag cgccggcccg agcacatcgc gtcgcgcacc ttcacgccgg tctgaccgac
     4321 cgctgatctc cccggagacg gggaaacctc gagggccacc caccgacgga cgggggtggc
     4381 cctcggcgcg tccggggtga cgcgtgtcac catgcgcagg acatcgcgca tcgcaggggg
     4441 aaccgcacgg acatgcagca gctcagtggc gtggacgcct cgttcctgta catggagagc
     4501 ccgaccacgt tcgggcacgt ctcctcgctg atcgtctacg accccaagga cagcgacagc
     4561 gccggctacg aggcgacccg ggcgctgttc caggagcggc tccacctgct cgacccgatg
     4621 cggcgccgcc tggtgcacgt gcccttcgac ctcgaccacc cgtactgggt ggaggacccc
     4681 gagctcgacc tcgacttcca catccgccag accgcggtcg ccccgcccgg caccgacgag
     4741 cagctgtccg agctggtcag ccgcatcatc gcccggcccc tcgaccgcag ccggcccctg
     4801 tgggagctct acgtcatcta cggcctgtcc gacgggcgca tcgcccagct gaccaagatc
     4861 caccacgccg ccatcgacgg cgtcggcggc gcccagctgc tcgccaacat cctcgacctc
     4921 gaacccgacg cgccaccccg gcccggcccg tgccccgacc ccgagcgcat ccccacccag
     4981 gaggagatgc tggtgcgagg gctgacgtcg ctggcgaccc ggccctacaa gcagggtcgc
     5041 ctggccgtcc ggatgctgcg ggagctgccc cagatcggc
//
LOCUS       DATISU010000259         5793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_480981, whole genome shotgun sequence.
ACCESSION   DATISU010000259 DATISU010000000
VERSION     DATISU010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480981"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1180)
                     /locus_tag="VLR27_10310"
     CDS             complement(83..1180)
                     /locus_tag="VLR27_10310"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HSP03885.1"
                     /translation="MINLRYHIVSLTAVFLAIGIGLTLGSTFLDRATVENLNGQLENL
                     ETRLGERDERIGVLEDELTEAEALQNALDEQGSGLLAGRLDEVPVVVVASSGVDQADV
                     DGAVESLQTAGADVQGEWWMTERLLLADQADIDDLAAVLEETSTDPARLRRLAIEGLG
                     REVQASQRLDAGVDTAAEAPLGDDEGVDPSTGDVEVEATDPTDPDGSDDADVEEPGDA
                     EDTVAAEGSLAQGLVDAGFIVFEAVAGGPEALTLPDGVRVVVVGGSPAVPDDVFVQPF
                     VDRLTQGVDRPTRTVVTSALDDGAAVSEAVAAIRADDDLRAVVSTVDRLDHFQGWMAT
                     VLAVEDLTDGTIGHYGLGEGAAALLPPLQDP"
     gene            complement(1177..2322)
                     /gene="steA"
                     /locus_tag="VLR27_10315"
     CDS             complement(1177..2322)
                     /gene="steA"
                     /locus_tag="VLR27_10315"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HSP03886.1"
                     /translation="MTQPTAVSGEPSLTGVARVDRRTKALIGRLQPGEIAVIDHEDLD
                     GVAAETLVGARPAAVVNASSSMTGRYPNIGPLALTEAGIPLIDAAGAEVMAIEEGTRI
                     TIRDGTILLGDDEIATGERQTHEGLQARILAAKSTMGAELERFAENTLRYIQAEGHLL
                     VDEPDVPEVPGVDVRGRHVLIVVRGVGYKDDLNLLRRSGYIADQKPVLIAVDGGADAL
                     LEVGRKPDVIIGDMDSVSEEALRCGARLIVHGYRDGRAPGGERLDDLGLDYEVYGSEG
                     TSEDIAMLLSYEKGCELIVAVGTHNSMVEFLDKGRAGMASTFLVRMKVGMMLVDAKGV
                     SRLYRSTVRTRDLFLLVLAAVFTMVVIVLVSEPIRLVIRTYWVSFTS"
     gene            complement(2319..3920)
                     /gene="recN"
                     /locus_tag="VLR27_10320"
     CDS             complement(2319..3920)
                     /gene="recN"
                     /locus_tag="VLR27_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HSP03887.1"
                     /translation="MIAELAVRDLGVFADARLVLDEGLTALTGETGAGKSLVVGAIQL
                     LLGARADASVVRPGAEEAIVDGRFVTAEGEEVVLSRVIPAVTGGRSRAYVNGRPVTVS
                     ELAERGGALVELHGQHAHQQLLQASAQRHALDRFAGVDLQPLRDARAQVAAVSAQLEE
                     LGGDERRRAREIDLLRYQLDELDQADLEDPDEDDRLDEEEDLLADAVAHRDAAAAAAE
                     ALVGDEGVAHQLQSVIGLVGGRGPFVELEGRLRSAAAEVTEAAHDLRDVAEGIADDPE
                     RLVAIRQRRQLLSELRRKYGDTLAEVLDEREAIRRRLDELLGHDARAEALEAELAAAR
                     AAEATAAAAVAKARKAAAPVLAGGVEERLRALAMPDARVEVVVEGEGPADDVRFLLAA
                     NPGGAPAPISKVASGGELSRATLALRLELTAGPPSLVFDEVDAGIGGETAWAVATALA
                     ALAGRHQILVVTHLAQVAAFADRQLRVAKSATDTSTTATVSVLDSDDRVEEIARMLSG
                     QPESEVGREHALDLLEAAADARRQA"
     gene            complement(3931..4779)
                     /locus_tag="VLR27_10325"
     CDS             complement(3931..4779)
                     /locus_tag="VLR27_10325"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HSP03888.1"
                     /translation="MVTAGFILHSERDRAASITHDLATWLLQRGHDVRMPEDDAERLG
                     LADHGVAEDHFADGLDVAVSLGGDGTMLRTVELVADGGIPVIGINVGQLGYLTEVDPA
                     GARMAIKRFLSGAYEIEERMLLSVRIEGRESSEHVALNEAVIEKTPSGHTVRLAVSVD
                     GEYFTTYAADGLIVATPTGSTAYSFSARGPIVAPMHRCLVLTPVSPHMLFDRTLVLDP
                     HSTLHLEVVGHRPATVSADGRNLGTLRPGDSLSCTASPRTARLVTFGEHDFLAILKTK
                     FGLSDR"
     gene            complement(4779..5618)
                     /locus_tag="VLR27_10330"
     CDS             complement(4779..5618)
                     /locus_tag="VLR27_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HSP03889.1"
                     /translation="MTSGARRRLDLELLRRGLTESRQQARHLIDAGRVLVSNAPADKA
                     GRLVAPGEPIRVLGPPARYVGRGGQKLAGALARFDLDPTGVRALDAGASTGGFTDALL
                     QAGAASVVAVDVGRAQLHERLRADERVDVREQTDIRTVRAEDVGGPVDVVVADLSFIS
                     LHAVLVPLLALVAPGGDLVVLVKPQFEAGRAEVSRGRGVIRDPQVWRRVLDEVMVAIR
                     DAGGAIMDAMVSPLRGADGNAEFLVHVRRPEHGRDPHEGAVPGRELDLDALVAEAAGE
                     ATP"
BASE COUNT          746 a         2089 c         2176 g          782 t
ORIGIN      
        1 ccggtgatgc ggctcaccgc cgtcagcacg gtctgcgtcg ccgtcgagcg ggcgaggctg
       61 cgccgctcgt cgacgggggc ggtcacgggt cctggagcgg cggcaggagc gcggcagcgc
      121 cctcgcccaa gccgtagtgg ccgatcgtcc cgtcggtcag gtcctcgact gcgaggaccg
      181 tcgccatcca gccctggaag tggtcgaggc ggtccacggt ggacacgacg gcccggaggt
      241 cgtcatcggc gcggatcgcc gcgaccgcct ccgacacggc ggccccgtcg tcgagcgcgg
      301 acgtgaccac ggtgcgggtg ggacggtcga cgccctgggt caggcggtcg acgaagggct
      361 ggacgaagac gtcgtcgggc accgcagggg agccgccgac gacgacgaca cgcaccccgt
      421 cgggcagcgt cagcgcctcc ggtcccccgg ccaccgcctc gaagacgatg aagccggcgt
      481 cgaccaggcc ctgggcgagc gaaccctccg ccgccacggt gtcctcggcg tccccgggct
      541 cctcgacgtc ggcgtcgtcg gagccgtccg gatcggtcgg gtccgtggcc tcgacctcga
      601 cgtccccggt cgaggggtcc accccttcgt cgtccccgag cggcgcctcg gcggcggtgt
      661 ccaccccggc gtcgaggcgc tggctggcct ggacctcccg gcccaggccc tcgatcgcca
      721 ggcggcgcag gcgggccggg tccgtggagg tctcctcgag gacggcggcg aggtcgtcga
      781 tgtcggcctg gtctgccagc agcagccgct cggtcatcca ccactccccc tgcacgtcgg
      841 cgccggcggt ctggagggac tccacggcgc cgtcgacgtc ggcctggtcg acgccggagg
      901 aggcgacgac caccaccggc acctcgtcga ggcggccggc gaggaggccg gacccctgct
      961 cgtcgagggc gttctggagc gcctcggcct cggtgagctc gtcctcgagc accccgatgc
     1021 gctcgtcccg ctcgccgagg cgggtctcca ggttctccag ctggccgttc aggttctcga
     1081 ccgtcgcccg gtcgaggaac gtcgagccca gcgtgaggcc gatcccgatg gcgaggaaga
     1141 ccgccgtgag cgagacgatg tggtagcgga ggttgatcat gaggtgaacg agacccagta
     1201 cgtgcggatg accagccgga tcggctcgct gacgaggacg atcacgacca tggtgaagac
     1261 ggcggcgagg acgagcagga acaggtcccg ggtccggacc gtggaccggt agaggcggct
     1321 gacgcccttg gcgtcgacca gcatcatgcc caccttcatg cgcacgagga aggtggaggc
     1381 catgccggcc cggcccttgt cgaggaactc gaccatcgag ttgtgcgtcc cgacggcgac
     1441 gatgagctcg cagcccttct cgtaggacag cagcatggcg atgtcctcgc tcgtgccctc
     1501 cgagccgtag acctcgtagt cgagaccgag gtcgtccagg cgctcgccgc ccggcgctcg
     1561 gccgtctcgg tacccgtgca cgatgagccg ggcgccgcag cgcagcgcct cctccgacac
     1621 cgagtccatg tcgccgatga tcacgtcggg tttgcggccg acctcgagca gcgcgtcggc
     1681 accaccgtcg accgcgatca ggacgggctt ctggtcggcg atgtagccgg agcggcgcag
     1741 gaggttcagg tcgtccttgt agcccacgcc gcgcaccacg atgagcacgt ggcgcccgcg
     1801 gacgtcgacg ccggggacct cgggcacgtc gggctcgtcg acgagcaggt ggccctcggc
     1861 ctggatgtag cgcagcgtgt tctcggcgaa gcgctcgagc tcggcgccca tggtcgactt
     1921 cgccgccagg atccgggcct gcaggccctc gtgcgtctgg cgctcgccgg tggcgatctc
     1981 gtcgtcgccc aggaggatcg tgccgtcacg gatcgtgatg cgcgtgccct cctcgatggc
     2041 catcacctcg gcgccggccg cgtcgatgag cgggatgccg gcctcggtca gcgccagggg
     2101 gccgatgttc gggtaccgcc cggtcatcga cgacgacgcg ttcaccacgg cagccggccg
     2161 ggcacccacg agggtctcgg ccgcgacccc gtcgaggtcc tcgtggtcga tgaccgcgat
     2221 ctccccgggc tgcagccggc cgatcagggc cttggtccgt cggtcgacac gcgccacacc
     2281 ggtgagggag ggttcgccgg acactgcggt gggctgggtc atgcctgcct ccgcgcgtcg
     2341 gcggccgcct cgagcaggtc gagggcgtgc tcccggccga cctcgctctc gggctgcccg
     2401 gacagcatcc gggcgatctc ctccacccga tcgtcgctgt cgagcaccga cacggtggcg
     2461 gtggtcgagg tgtcggtggc ggacttggcc acgcgcagct ggcggtcggc gaaggcggcg
     2521 acctgggcga ggtgggtcac gacgaggatc tggtggcggc cggccagcgc ggcaagggcg
     2581 gtggccaccg cccacgccgt ctcgcccccg atgccggcgt cgacctcgtc gaacaccagc
     2641 gagggcggac ccgcggtgag ctcgaggcgc agcgcgagcg tggctcggga cagctcgccg
     2701 ccggaggcga ccttggagat cggggccggt gccccacccg ggttggcggc cagcaggaac
     2761 cgcacgtcgt cggccggacc ctcgccctcg accacgacct ccacgcgggc gtcgggcatc
     2821 gccagggctc ggaggcgctc ctcgacgccg ccggccagca ccggtgccgc agccttgcgg
     2881 gccttggcga cggcagcagc ggccgtggcc tcggccgcac gggcggcggc cagctcggcc
     2941 tccagcgcct cggcgcgggc gtcgtgaccc agcagctcgt ccaaccggcg acggatcgcc
     3001 tcgcgctcgt cgaggacctc ggccagggtg tcgccgtact tgcggcgcag ctcggacagc
     3061 agctggcggc gctgccggat ggcgacgagg cgctccgggt cgtcggcgat gccctcggcg
     3121 acgtcgcgca ggtcgtgggc cgcctccgtg acctcggcgg ctgcggagcg caggcggccc
     3181 tcgagctcga cgaagggacc acggccgccg accaggccga tgaccgactg gagctggtgg
     3241 gccacgccct cgtcgcccac cagggcctcg gccgccgctg cggctgcgtc ccggtgcgcg
     3301 accgcgtcag ccaggaggtc ctcctcctcg tcgaggcggt cgtcctcgtc cgggtcctcc
     3361 aggtcagcct ggtcgagctc gtcgagctgg tagcgcagca ggtcgatctc gcgcgcccgt
     3421 ctgcgctcgt cgccgccgag ctcctcgagc tgggccgaca ccgccgcgac ctgcgcccgg
     3481 gcgtcgcgga ggggctgcag gtcgaccccg gcgaagcgat cgagggcgtg gcgctgcgcg
     3541 gaggcctgca ggagctgctg gtgggcgtgc tggccgtgaa gctcgaccag ggcgccaccc
     3601 cgctcggcca gctcggacac ggtgaccggc cgcccgttca cgtaggcccg gctgcgcccg
     3661 cccgtgacag ccgggatcac gcgggacagg acgacctcct cgccctcggc cgtcacgaag
     3721 cgaccgtcga cgatggcctc ctcggccccg ggtcggacca ccgaggcatc ggcgcgagca
     3781 cccagcagca gctggatcgc gcccacgacc agcgacttgc cggcgccggt ctcgccggtc
     3841 agggcggtga ggccctcgtc gagcacgagc cgggcgtcgg cgaacacgcc gaggtcccgg
     3901 acggccagct cggcgatcac gaggtggtcc tcatcggtcg gacagcccga acttggtctt
     3961 caggatggcc aggaagtcgt gctcgccgaa ggtgacgagc cgggcggtgc gggggctggc
     4021 cgtgcagctg agcgagtcgc cggggcggag ggtgccgagg ttgcgcccgt cggccgagac
     4081 ggtggccggt cggtgcccga cgacctccag gtgcagggtc gagtgcgggt cgaggacgag
     4141 cgtgcggtcg aagagcatgt gcggcgacac cggcgtgagc accaggcacc ggtgcatggg
     4201 cgccacgatg ggcccccggg ccgagaacga gtaggccgtc gagccggtcg gggtggccac
     4261 gatcaggccg tcggcggcgt aggtcgtgaa gtactcgccg tcgacgctca cggcgaggcg
     4321 caccgtgtgg ccggaggggg tcttctcgat gacggcctcg ttcagggcca cgtgctcgga
     4381 tgactcgcgc ccctcgatcc ggaccgagag cagcatccgc tcctcgatct cgtaggcgcc
     4441 ggagaggaag cgcttgatcg ccatccgggc gccggccgga tcgacctcgg tgaggtagcc
     4501 gagctggccc acgttgatgc cgatcaccgg gatgccgccg tcggcgacca gctccaccgt
     4561 gcgcagcatg gtgccgtcgc cgccgaggct gaccgccaca tcgaggccgt cggcgaagtg
     4621 gtcctcggcc accccgtgat cggcgagacc gagccgctcg gcgtcgtcct cgggcatccg
     4681 cacgtcgtgg ccgcgctgga gcagccaggt ggccaggtcg tgggtgatgg acgcggcacg
     4741 gtcgcgctcg ctgtgcagga tgaagccggc cgtcaccatc agggggtcgc ctccccggcg
     4801 gcctcggcga cgagcgcgtc gaggtccagc tcgcgccccg ggaccgcacc ctcgtgcggg
     4861 tcgcgaccgt gttcggggcg acggacgtgc acgaggaact cggcgttgcc gtccgccccg
     4921 cggagggggg agaccatggc gtccatgatg gcgcctccgg cgtcgcggat ggcgaccatc
     4981 acctcgtcca ggacgcgccg ccagacctgc gggtcacgga tgacgccgcg cccacgggac
     5041 acctcggcgc ggcccgcctc gaactgcggc ttgaccagga cgacgagatc gccgcccggg
     5101 gcgacgaggg ccagcagggg cacgagcacg gcgtggagcg agatgaacga caggtcggcg
     5161 accacgacgt cgaccgggcc gccgacgtcc tcggcgcgca ccgtccggat gtcggtctgc
     5221 tcccggacgt cgacccgctc gtcggcgcgg agccgctcgt ggagctgggc ccggcccacg
     5281 tcgacggcga ccaccgatgc cgcgccggcc tgcaggagcg cgtcggtgaa gccgccggtg
     5341 gaggcaccgg cgtccagggc ccgcacgccc gtgggatcga ggtcgaagcg ggccagcgcc
     5401 ccggccagct tctggccgcc gcgccccacg taccgggccg gcggaccgag cacgcggatc
     5461 ggctccccgg gcgcgacgag gcgaccggcc ttgtccgccg gcgcgttcga cacgagcaca
     5521 cgcccggcgt cgatcaggtg tcgggcctgc tgtcggctct cggtgagccc gcgccggagc
     5581 agctcgaggt cgagtcgccg ccgggcgccg gaggtcatgc ctcctacgag gccgacgagc
     5641 cgccggtcga cccgggtgcg gccttcttgg cggcggcctt cttcgtggcg gccttcttgg
     5701 ccggcgcctt cttcttggtc gccgacttct tggcggtggt ccgcttggca gccgccttct
     5761 tggtggtccg cttcttcgcg gcagccttct tcg
//
LOCUS       DATISU010000260         3827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_247332, whole genome shotgun sequence.
ACCESSION   DATISU010000260 DATISU010000000
VERSION     DATISU010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247332"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VLR27_10335"
     CDS             complement(<1..199)
                     /locus_tag="VLR27_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HSP03890.1"
                     /translation="MSTTDEARVTEAVEQLLAETHPKQVDKVTVRGAVYDAGLAWIHF
                     PEGWGGMGLAPTHQRIVEKALR"
     gene            314..1546
                     /locus_tag="VLR27_10340"
     CDS             314..1546
                     /locus_tag="VLR27_10340"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSP03891.1"
                     /translation="MALEPTIDPTEVGVDPGRLARIDELTHAYVDDGRFPFVQLLVTR
                     RGEIVHHDLYGYADRDSDRPIRDDCIVRVYSMTKPVTSVALMMLYEQGKVLLEDPVSK
                     YLPAFAEPRVFTSGNQQRYETREASREITVHDVLTHMSGLTYGFQNQHAVDAIYRTHG
                     LGDFSPSTRSLSEEMELLGSLPLQFDPGTSWCYSMSTDVCGAIVEVVSGMALDEFFER
                     EIFEPLKMPDTSFWVEGEDRCARFTTNHLFFGGTTSVMDPWDKSLYHRRPPMLSGGGG
                     LVSTMADYHRFVRMLERGGELDGVRLLSPRTVAFMASNHLPGGRDLNDMGQVGFAEVA
                     MEGMGFGLGFSVSLSPQENRAIGSPGDYGWGGAASTSFRIDPAEELSFVFLTQLLPSS
                     TYPIRRELQATIYQALVD"
     gene            1546..3090
                     /locus_tag="VLR27_10345"
     CDS             1546..3090
                     /locus_tag="VLR27_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSP03892.1"
                     /translation="MEQATRILSAAGLSGDVLTGGDLDVRTPIDGSLLASIATVDASD
                     VDAAVQRASDAFRTWRDVPAPRRGELVRLLGEELRAAKDDLGALVSLECGKIRQEGLG
                     EVQEMIDICDFAVGLSRQLHGLTIASERPGHAMRETWHPMGPCAVITAFNFPVAVWAW
                     NTALALACGDPVIWKPSEKAPLTALATLGIFRRAAERFGDAPDDLVQVVVGDAEVGRA
                     LAEHPDVAIVSATGSTRMGRAVSQSVAARLGRSILELGGNNAMIVAPSADVDLALRAV
                     VFSAVGTCGQRCTSLRRLIVHDDVADQLLPALERTYAGLPVGDPLAEGTLVGPLIDGA
                     AFDAMQAALDRAREQGGTVVGGERITDAVPAADDGEGFYVRPAIVHMPEQTDLVRTET
                     FAPILYVLRYRDLDDAIAMHNDVPQGLSSCIFSTDVRETERFLSATGSDCGIANVNIG
                     PSGAEIGGAFGGEKDTGGGRESGSDAWKGYMRRQTATVNWSRELPLAQGIVFDVEGGT
                     TEEAPA"
     gene            3090..>3827
                     /locus_tag="VLR27_10350"
     CDS             3090..>3827
                     /locus_tag="VLR27_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HSP03893.1"
                     /translation="MALHSIAVLGLGKVGLLAARLLDELGYDVSGHDLRTPHEDLPFP
                     VLSTDVSDADELSRALAGSDAVLSCLPYHCNRLVAEVAHGLGMHYFDLTEDTATTEAI
                     MALAETATGLMAPQCGLAPGFVGIVAASQAESFDHCRAIRMRVGALPQHPTGLLGYAF
                     NWSPEGVVNEYLNDCEVIEDGVRKWVSPMEWKEVVYVAGTKLEAFTTSGGLGTMCDTY
                     LGRVENLDYKTMRYPGHMDLMNFFFHEL"
BASE COUNT          543 a         1436 c         1269 g          579 t
ORIGIN      
        1 cgcgcagcgc cttctcgacg atccgctggt gcgtcggggc caggcccatc ccgccccaac
       61 cctcggggaa gtggatccag gccaggccgg cgtcgtacac ggcaccgcgg accgtgacct
      121 tgtcgacctg cttcgggtgg gtctcggcga gcagctgctc gaccgcctcg gtgacgcgtg
      181 cctcgtcggt ggtgctcatc ggttccccct ggtgtcgcgg tcgcgatcgt cgttcggtgg
      241 catctgagcc ggtgatcgcg cgaccggtca agatcgacca cctgccgtgg ggaggcccgc
      301 gtagggtccg gccatggcgc tcgaacccac catcgatccc accgaggtcg gggtcgaccc
      361 cggccggctc gcccgcatcg acgagctcac gcatgcctac gtcgacgacg gtcgcttccc
      421 cttcgtgcag ctcctcgtga cccgccgggg ggagatcgtc caccacgacc tctacggcta
      481 cgccgaccgc gacagcgacc ggccgatccg cgacgactgc atcgtgcgcg tgtactcgat
      541 gaccaagccg gtcacgagcg tggcgctgat gatgctctac gagcagggca aggtcctgct
      601 cgaggatccg gtctcgaagt acctgcctgc gttcgccgag ccgagggtct tcacgtcggg
      661 caaccagcag cgctacgaga cccgcgaggc gtcccgggag atcaccgtcc acgacgtgct
      721 cacccacatg tcgggcctca cctacggctt ccagaaccag cacgccgtcg acgccatcta
      781 ccggacccac ggcctcggcg acttcagccc gtcgacccgc agcctcagcg aggagatgga
      841 gctgctcggc agcctgccgc tgcagttcga tcccggcacc agctggtgct actcgatgtc
      901 caccgacgtc tgcggtgcca tcgtcgaggt cgtgagcggc atggccctcg acgagttctt
      961 cgagcgcgag atcttcgagc cgctgaagat gcccgacacc tcgttctggg tcgagggcga
     1021 ggaccgctgc gcccgcttca ccaccaacca cctgttcttc ggcggcacca ccagcgtgat
     1081 ggacccgtgg gacaagtcgc tctaccaccg gcgaccgccg atgctctccg gcggcggcgg
     1141 gctggtgtcc accatggccg actaccaccg gttcgtccgg atgctggagc gcggcggcga
     1201 gctcgacggg gtccggctgc tgtcaccccg caccgtcgcc ttcatggcca gcaaccacct
     1261 gccgggtggc cgggacctca acgacatggg ccaggtcggc ttcgccgagg tggcgatgga
     1321 gggcatgggc ttcggcctcg gcttcagcgt cagcctctcg ccgcaggaga accgtgccat
     1381 cggctccccc ggcgactacg gctggggcgg cgctgcgtcc acctcgttcc gcatcgaccc
     1441 ggccgaggag ctcagcttcg tgttcctcac ccagctgctg ccgtcgtcga cgtatccgat
     1501 ccggcgtgag ctgcaggcca ccatctacca ggcactggtg gactgatgga gcaggccaca
     1561 cgcatcctct ccgccgccgg cctgtccggc gacgtgctca ccggcggcga cctcgacgtc
     1621 cgcacgccca tcgacgggag cctcctggcg tcgatcgcca cggtcgacgc ctccgacgtc
     1681 gatgccgccg tccagcgggc atccgatgca ttccgcacct ggcgggacgt gccggccccg
     1741 cgacgtggcg agctcgtccg gctgctcggc gaggagctgc gcgccgccaa ggacgacctc
     1801 ggcgccctcg tctcgctgga gtgcgggaag atccgccagg agggcctcgg cgaggtccag
     1861 gagatgatcg acatctgcga cttcgccgtg ggcctgtcgc gccagctcca cggcctcacc
     1921 atcgcgtcgg agcggccggg tcacgccatg cgggagacct ggcacccgat gggcccctgc
     1981 gccgtcatca ccgcgttcaa cttcccggtg gcggtgtggg cgtggaacac cgccctcgcc
     2041 ctggcgtgcg gcgaccccgt gatctggaag ccgtcggaga aggcgccgct caccgcgctc
     2101 gccactctcg gcatcttccg gcgcgcggcc gagcgcttcg gcgacgcacc ggacgacctc
     2161 gtccaggtgg tcgtgggcga cgccgaggtc gggcgggcgc tggccgagca tcccgacgtg
     2221 gcgatcgtgt ccgccacagg ctccacccgc atgggccggg ccgtctccca gtccgtcgcc
     2281 gctcgcctcg gacgcagcat cctcgagctc ggcggcaaca acgccatgat cgtggcgccc
     2341 tccgccgacg tggacctggc gctgcgggcc gtggtcttct ccgcggtcgg cacgtgcggc
     2401 cagcgctgca cgagcctgcg ccggctcatc gtgcacgacg acgtcgccga ccagctgctg
     2461 cccgcgctcg agcgcaccta cgccgggctg ccggtgggcg accccctcgc cgagggaacc
     2521 ttggtgggcc cgctcatcga cggcgccgcg ttcgacgcca tgcaggccgc gctcgaccgg
     2581 gcccgcgagc agggcggcac cgtggtcggt ggcgagcgca tcaccgatgc ggtcccagcc
     2641 gcagacgacg gcgagggctt ctacgtccgc ccggccatcg tgcacatgcc cgagcagacc
     2701 gacctggtcc gcaccgagac cttcgccccg atcctctacg tgctgcggta ccgggacctc
     2761 gacgacgcca tcgccatgca caacgacgtc ccccagggcc tgtcgtcgtg catcttctcc
     2821 accgacgtgc gcgagaccga gcggttcctg tcggccaccg gctcggactg cggcatcgcc
     2881 aacgtcaaca tcgggccgtc cggggccgag atcggcgggg cgttcggcgg cgagaaggac
     2941 accggcggcg gccgggagtc cggctccgac gcctggaagg gctacatgcg ccgccagacc
     3001 gccaccgtca actggtcccg ggagctgccg ctcgcccagg gcatcgtgtt cgacgtcgag
     3061 ggcggcacca ccgaggaggc ccccgcctga tggcactgca ctcgatcgcc gtgctcggcc
     3121 tcggcaaggt cgggctgctg gccgcccgcc tgctcgacga gctcggctac gacgtcagcg
     3181 gccacgacct gcgcacaccg cacgaggacc tgccgttccc ggtcctcagc accgacgtgt
     3241 ccgacgccga cgagctcagc cgagccctgg ccgggtccga cgccgtgctc tcctgcctgc
     3301 cgtaccactg caaccggttg gtggccgagg tggcccacgg gctcggcatg cactacttcg
     3361 acctcaccga ggacacggcc accaccgagg cgatcatggc gctggccgag accgccaccg
     3421 ggctgatggc cccccagtgc ggcctggcgc cgggcttcgt cggcatcgtg gccgcgtctc
     3481 aggccgagtc cttcgaccac tgccgcgcca tccgcatgcg ggtcggcgcg ctcccccagc
     3541 accccaccgg cctgctcggc tatgccttca actggtcccc ggagggggtg gtgaacgagt
     3601 acctcaacga ctgcgaggtc atcgaggacg gcgtgcgcaa gtgggtctcg ccgatggagt
     3661 ggaaggaggt cgtctacgtc gccggcacca agctcgaggc gttcaccacg tccggtgggc
     3721 tcggcaccat gtgcgacacc tacctcggtc gggtcgagaa cctcgactac aagaccatgc
     3781 ggtacccggg ccacatggac ctgatgaact tcttcttcca cgagctg
//
LOCUS       DATISU010000261        14679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_435002, whole genome shotgun sequence.
ACCESSION   DATISU010000261 DATISU010000000
VERSION     DATISU010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 14679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14679
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435002"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..619
                     /locus_tag="VLR27_10355"
     CDS             59..619
                     /locus_tag="VLR27_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03894.1"
                     /translation="MDIHQCPRCELRFVNTSELRHHFEFDHAADPRVFERYRYRSAGT
                     DDAARHTILLVGNQTLERDAVLDEVVTRAADGAQVLVVVPATQHRPDDAEDMGAALAR
                     HRLAATVERLRAAGIDADGEVGDADPYAAVCHALGGHDIDEILLSTLPPSSSRWLSVD
                     LPGRLRRHTTRPVTVLTPASAGREAS"
     gene            complement(647..1042)
                     /locus_tag="VLR27_10360"
     CDS             complement(647..1042)
                     /locus_tag="VLR27_10360"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HSP03895.1"
                     /translation="MIFEETVVLDVPFDEALARTKEAFAAEGFGTLTEIDVQATLAEK
                     IDKEMDPYVIVGACNPGLAGRAIDAEPQIGVLLPCNVVVRQTGGNVVVEAMDPGLMAT
                     IVGTEAIRPIADEARRLVSNAMERLASSQ"
     gene            1232..1498
                     /locus_tag="VLR27_10365"
     CDS             1232..1498
                     /locus_tag="VLR27_10365"
                     /inference="COORDINATES: protein motif:HMM:NF020532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dsRBD fold-containing protein"
                     /protein_id="HSP03896.1"
                     /translation="MTSHPVWRIEITFTEDDSKTRADAVLDIAGNHHHGWGRSRRNPT
                     DQDVPIIGEEVAAARALIRLAHQLLGAAANDIEEIEHHPVQVHA"
     gene            1550..2824
                     /locus_tag="VLR27_10370"
     CDS             1550..2824
                     /locus_tag="VLR27_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HSP03897.1"
                     /translation="MTTSPAASAAAVLDHIAPGTNVIVPLANGEPVSLLDALQEHAAG
                     LDRVTVHQMHGLYDRPFLHGANRGRLDYRAYFLSHVTRKAFHDGGCDLVPCHFSEVPQ
                     LLEGLTGPKIVLAASSPIDRHGCFSLGTNADYVASFIGRVPFFLEANVQMPRTFGRNQ
                     LHVSQVAGWVEVDRPLIEVPPPESNELDDRIAAQVAERIPDGATVQVGIGSIPNAILH
                     RLGDHRDLGLHTELLSDGVIPLVESGVLNGTRKRRRPTKLVATFALGTRALYDFMDEN
                     TIVDMLPVDWVNDPRIIGQDDCFVSVNATTEIDFLGQAASETIGGRYYSGSGGQTDFA
                     RGAQYSRNGQGFIVLHSTTNDGTKSRISAQLPPGSVVTTLKNTIDNVVTEWGVAELRG
                     RSLRERAAALIRVAHPDFRDQLSREARELGYL"
     gene            complement(2839..3996)
                     /locus_tag="VLR27_10375"
     CDS             complement(2839..3996)
                     /locus_tag="VLR27_10375"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP03898.1"
                     /translation="MGEHTFTERARALGPELRDRSDEIDSLRRLPDDLVDGLTDEGFF
                     RFWVPKEYGGAEISLLDGLETFMELARHDTAVAWCCFIANTTALTAGLLEPRWAEELF
                     RPPGAVAGGFAAPVGRARVVDGGLRVTGRWQWGSGTQHCTVIGGGVLLVDDDGAVAPR
                     DDGLLAPFVLLDRDDVTFHDTWHVLGVRGSGSTDYEVTDAFVPEGRWVELTRPTTHVD
                     GPLYRFSLFSLLAGGIGCTAIGVAERALEEFTTLARGKVPQGSGRTLSERPSAQADVA
                     KAEATIASARLFLLDAYADAWATAEAGDEPSIEQRRLVRLATTDATQRCADVVSRLHR
                     AAGGEAVYQRCPLERLFRDANVVSQHAMAAERTYELAGRLAFGLDSDTSTL"
     gene            complement(4012..4407)
                     /locus_tag="VLR27_10380"
     CDS             complement(4012..4407)
                     /locus_tag="VLR27_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSP03899.1"
                     /translation="MTGWVDDIEKATLENDTFRTVLFTGAHLQMTVMCIQPGEEIGVE
                     KHDDLDQFIRIEQGSGQVQMGPAKDDLSEVRDIEDDWAAIIPGGTWHNVVNTGSEPLR
                     LYSIYTPPEHPEGTVHETKAEADAAEHDH"
     gene            4528..6063
                     /locus_tag="VLR27_10385"
     CDS             4528..6063
                     /locus_tag="VLR27_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP03900.1"
                     /translation="MRVPLTVRDHIDRAELVYGDRIAFVDEPDQPAQSWGSVTYREMA
                     ARARSMAVAFDQMGVKHGERVAIVSHNSARLLTAFFGVSAYGRVLVPINFRLNREEIA
                     YIVEHSGASVLLIDPALADDLAGVEAEHTMVLGADTDQQLFRAVGEPIPWEPDEDATA
                     TINYTSGTTARPKGVQLTHRSLWLNAATFGWHMGVSDRDVYLHTLPMFHCNGWGMVYA
                     VTGMGGRHIVLRQIDGPEILRRIDDHGVTLLCGAPAVGSIVLDAASSLGDDVPGRGTT
                     RWVMAGAPPPTATIEAVERDLGWEFVQIYGLTETSPLLTINRGRAEFDDLDPTERAQK
                     LSRAGAPAIGVQLSTSESGEVLARSNVVLEGYWEQPDASAAALEGGWFHTGDGGFLDD
                     DHYLHISDRKKDVIISGGENVSSIEVEDCLFSHPDVAEVAVIGVPDEKWGEMVKALVV
                     LAPGATADEAALIEHCRSKLAHYKCPKSIDFRDELARTATGKLQKFKLRAPFWEGQER
                     QVN"
     gene            6096..6575
                     /locus_tag="VLR27_10390"
     CDS             6096..6575
                     /locus_tag="VLR27_10390"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HSP03901.1"
                     /translation="MRIRRPPLLLLALLAALVAVAAPAAAHTQIQRATPGPGESVSGE
                     LDVVVLDFLDPVVPTPEITVTGPDGALVAGLQPAELIADDVAVARFDPLTQAGQYQVD
                     YTFVALDGDEQVSAHTFTFEPDQGLDLEVRPLLAWIVGGVLVALVVAALIGRRRARG"
     gene            6599..7006
                     /locus_tag="VLR27_10395"
     CDS             6599..7006
                     /locus_tag="VLR27_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03902.1"
                     /translation="MLPITLDTVRIFVHVLAATIWVGGQLTLAGLLPGLRTLGPDAPR
                     TIARRFNRIAWPAYGVLVATGVWNLFEVSFGDRDTDWQVTLFVKLVVVALSGLAAVTH
                     SMSASKAVLAIGGAMAGLSSLAALFLGVQLAFG"
     gene            7111..7416
                     /locus_tag="VLR27_10400"
     CDS             7111..7416
                     /locus_tag="VLR27_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSP03903.1"
                     /translation="MNKSELIEQVAKQAGVDKKTAGDVLKGFEEVVLAVTKAGKEEIS
                     WTGFLKFSQGKRKAGTARNPQTGESIKTKAKKIPKVTLGATFKKVVNGESPAPKLAK"
     gene            7548..7841
                     /locus_tag="VLR27_10405"
     CDS             7548..7841
                     /locus_tag="VLR27_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03904.1"
                     /translation="MRRLLATLALVSVLALGALAGAAVPVAAQTDGQVTTTTVPRGGD
                     IIPQPNSGSEPEDAGDRGGALQTALFIGLVGVIFVAGAVIVRQSRKARAERGF"
     gene            complement(7851..9209)
                     /locus_tag="VLR27_10410"
     CDS             complement(7851..9209)
                     /locus_tag="VLR27_10410"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HSP03905.1"
                     /translation="MRRRSLPLLLAVVLVAAATACTESDLTLRDGGDLVRPEPDIPPG
                     DVVTDPDEVTQLALDDLGAYWTAAMVEVYDTEFRPLAGGYVPYGPRAPLPACGPDPLT
                     YDQIAENALYCPAEDLIAWDRENLIPGLQERFGSLTVGLVMAHEFAHAVQARAGVRGA
                     TVTLELQADCFAGAWVDDVDDRIPTFHTDADSLDSAVAGLLELRDTLGVPGYDSSAHG
                     SGFDRVSAFQQGYEDGPAACATYADEPPAVVAIPFGSPTDAASGGNLPLDELYEPLVA
                     DLESFYAALLAEEGEDWDPVGGFEATDPADGAITCGDEEISEAELELAAFYCVADDTA
                     YMDGDELIPALAEIGDFAFGGELARLYAFAAQEQLGIEPDDEVVGALHADCLTGVFAA
                     AEFLQTVPEQRLVLSPGDLDEIIVAFLAFGGEAETSAFERTAAFRAGFVEGADPCRRL
                     VD"
     gene            complement(9234..9626)
                     /locus_tag="VLR27_10415"
     CDS             complement(9234..9626)
                     /locus_tag="VLR27_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03906.1"
                     /translation="MRQIWNDLGSKLGTGLCAAGVLLVFLGWNGAASVDRVEAQFPYL
                     LSGGIAGLSLVVMGIGLIIVQNQRSDRAMLQQTLRELEAAVRGEDPAATEFEAPAPRP
                     TPEIYAAALRPKAPARRARRAPLTAEDA"
     gene            complement(9629..10333)
                     /locus_tag="VLR27_10420"
     CDS             complement(9629..10333)
                     /locus_tag="VLR27_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP03907.1"
                     /translation="MALLEIHDLVVGYDFEVLHGVSFAVEEGETAVLFGLNGAGKTTT
                     VTTVAGMRAPWSGRIVFDGEDIGGRSPSDLVQAGISLSPEGRRLFPRLTVEDNLRMGN
                     WVRRNDKGVFDTQRDLVFEYFPRLAERRSQVAGTLSGGEQQMLAIGRALMSAPRLLLI
                     DEASLGLSPALAVVVFEVVKRICGDGTTVVIVEQNVGVLPYADRALIMEQGTIVYEGV
                     GEEIQSANLRETYLGS"
     gene            complement(10333..11139)
                     /locus_tag="VLR27_10425"
     CDS             complement(10333..11139)
                     /locus_tag="VLR27_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP03908.1"
                     /translation="MTSEREPSAETVLETSQVSIRFGGVQALQDVDLRADRWEIVGII
                     GPNGAGKTTLFNCITGFYEATSGHVRFKGEDVTDLPVHERATRGMGRTFQNVGLVKGS
                     TVGENLKSAQHLQFRYGALGALFGSTKASEVEREATERARAILDLVDLGHLWDTRVAG
                     LPYGIQKRLEIAAVLATDPELLLLDEPGSGLGPEEADDLGDFLLDIRERFRLTIVLID
                     HHVPLVTRISDYMYCLNLGQVLCEGQPDDVRNHPDVVRAYLGVDPDAEVA"
     gene            complement(11123..12280)
                     /locus_tag="VLR27_10430"
     CDS             complement(11123..12280)
                     /locus_tag="VLR27_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSP03909.1"
                     /translation="MTAVDPTVQLDVLRAPDGEDDPDETRTGPSTLGWVSRGLVWAFL
                     AWLVLLYPANIASPFQVPAFDANRMSEAVIFAIIGLSLNVLIGYAGQVSLGHQAFVGI
                     GAFTSAYVVTDLTQEVVLGVLVAALVGAVQSILLGLVSLRLTGLYFALVTLVYGVMAQ
                     QSIFAIEAFTGGTAGKIAPRPTGFESDHRYYYFALIGLVAVLLIDWRMLRTKGGRAIL
                     ALRENPRVAQTWGIDTKAYTLFAFAVAGLFAGLGGGLLAHRDLQVVANPFDFNLALVF
                     VLMTTVGGLRNRTGVVLGSMFFAFLRNGYLVEKLRLEEGLGPIPGPLDLIDLSPEFAP
                     LVIGPILLLVVLTRLPGGFGQLARPFQEWLAGRRFRWIPADEEVTVNDVRA"
     gene            complement(12277..13176)
                     /locus_tag="VLR27_10435"
     CDS             complement(12277..13176)
                     /locus_tag="VLR27_10435"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSP03910.1"
                     /translation="MLLGISGTELTQSLVIGTVNGAVYALIALSLVLVYRSSGIFNFA
                     QAEFGTVAVYGAYFSFSYWGFPYVVAALFGVVCGIVAALLTERFVIRPLSQGPKVTLL
                     VATAGIALLSIGVAFWRTGDNALRFFDPLTQWEGARVAGVQVSGQRMLTLLLLLVAVA
                     ALTAFFRSPYGLAVLAASQDADASALVGLDVNRISMLVWGIAGTLGAFAGILLQGGNA
                     FTPGAITSLALIPGFTAAVIGGLTSLPGAVLGGMVIGMLESFGRLSTFDVIPGASSVS
                     VFVVLLLVLLVRPEGLLGGRMAT"
     gene            complement(13179..>14679)
                     /locus_tag="VLR27_10440"
     CDS             complement(13179..>14679)
                     /locus_tag="VLR27_10440"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSP03911.1"
                     /translation="RTGALTTVNPVRRTHRRSATVAVLVALLLIAAACGQKTDVASVS
                     AGADPSSGFDDATGSTGGAFDDIDGATGATGGAKTSTAGRTTAGGTPATTPAGGGTGA
                     GGGGGGGEPGGGDPAADQGGGGAVPAGDRTGITDTEIVIGVHAPVTGAAPLPQSSFDE
                     AKDVYWQALNEQGGLFGRKVRVVFEDDQFDPRTAVAKCKKMVTEDKVFLLVGGGGADQ
                     ITACAQYAQSVGVPYLSAGVNEEGLSGLRAYFALSETYAQQSAKLVQMAQKQLGGGKF
                     GIAVADSPTFNDAHASIVRAAQDAGLDVVYNKRIPKGASQNQALGIAADMAGSGAEIV
                     YFLSSPVTFLNVAAASSGQAYRPQWIGPGVTSGLNTVANVGCSAANSVDKAIFFSPFP
                     QLDVIDQLDPQFRPAYQKHVGGDPDDLAIALWGLNKAVHQMFLAAGEGVTRQSFVQAL
                     EGGAAFETGVYPPLQYSAQNHFGARSVHVLQADCGSRTFKTIGTFVNGF"
BASE COUNT         2202 a         5092 c         5353 g         2032 t
ORIGIN      
        1 ccccttcccc gtccggatct cccgtcgtac cctgacggga ggccgaaggg ggaccgacat
       61 ggacatccac cagtgcccgc ggtgcgagct gcgcttcgtc aacacgagtg agctccgcca
      121 ccacttcgag ttcgaccacg ccgccgaccc gcgcgtgttc gagcgctacc ggtaccgatc
      181 ggccgggacg gacgacgctg cccggcacac gatcctgctg gtcggcaacc agaccctcga
      241 gcgcgacgcc gtcctcgacg aggtcgtgac ccgcgccgcc gacggtgcgc aggtgctcgt
      301 ggtcgtgccg gccacccagc accgccccga cgacgccgag gacatggggg ccgcgctcgc
      361 ccgccaccgg ctcgcagcca ccgtcgagcg gctccgggcc gccggcatcg acgccgacgg
      421 ggaggtgggt gacgccgacc cgtacgccgc cgtgtgccac gcgctcggcg ggcatgacat
      481 cgacgagatc ctgctgtcga cgctgccgcc gtcgtcgtca cgctggctga gcgtcgacct
      541 gcccggacgc ctgcgccgcc acaccacgcg cccggtcacg gtgctcacgc ccgcgtcagc
      601 ggggcgggag gcgagctgag ccggtcgcgg cccgggcccc gccaggtcac tgcgaggagg
      661 cgagccgctc catggcgttg ctgacgaggc ggcgggcctc gtctgcgatc ggtcggatcg
      721 cctcggtgcc gacgatggtg gccatgaggc cggggtccat ggcttcgacc accacgttcc
      781 cgccggtctg gcgcacgacg acgttgcagg ggagcagcac gccgatctgg ggttcggcat
      841 cgatggcccg gccggcgagg cccgggttgc aggctccgac gatcacgtag ggatccatct
      901 ccttgtcgat cttctcggcc agggtcgcct gcacgtcgat ctcggtgagg gtgccgaagc
      961 cctcggccgc gaaggcctcc ttggtcctgg cgagtgcctc gtcgaacggc acatcgagca
     1021 caacggtctc ttcgaagatc atgatcggat tcctcctcgt gggtggtgct gcgaccgtac
     1081 ggcctcatcg acggcggtgc ccagggtcat cggacaccag gcatcggggc acgacggtcg
     1141 aagggccctg gcgcgagcgc ggccggaggc gcacgcttgt cgtggcgggt cggatcccgc
     1201 ctccgggaac cacgtagtga ggagcgcagc catgacgtcg catccggtgt ggaggatcga
     1261 gatcacgttc accgaggacg acagcaagac gcgagccgat gcggtgctcg acatcgccgg
     1321 caaccaccac cacgggtggg gtcggagccg tcggaacccg acggaccagg acgtgccgat
     1381 catcggcgag gaggtggctg ccgcccgtgc gctgatccgc ctggcccacc agctgctcgg
     1441 cgccgccgcg aacgacatcg aggagatcga gcaccacccg gtgcaggtcc acgcctgacc
     1501 ggctcacctc gcgggtcgac cgcgccggcg tcggtcaaca tgaggggcca tgacgaccag
     1561 ccccgccgcc tccgccgccg ccgtcctcga ccacatcgcg cccgggacca acgtcatcgt
     1621 ccccctggcc aacggcgagc cggtgtcgct gctcgacgcg ctccaggagc atgccgccgg
     1681 cctggaccgg gtgaccgtcc accagatgca cggcctctac gaccggccgt tcctccacgg
     1741 ggcgaaccgc ggtcggttgg actaccgggc ctacttcctg tcccacgtca cacggaaggc
     1801 gttccacgac ggcggctgcg acctggtgcc gtgccacttc tccgaggtgc cccagctgct
     1861 cgagggcctg accgggccca agatcgtcct ggccgcgtcc tcgccgatcg accgccacgg
     1921 gtgcttctca ctcggcacca acgccgatta cgtcgcctcc ttcatcggtc gcgtgccgtt
     1981 cttcctcgag gcgaacgtcc agatgccgcg aaccttcggg cgcaaccagc tccacgtctc
     2041 gcaggtggcc ggctgggtcg aggtcgaccg tccgctcatc gaggtgccgc caccggagag
     2101 caacgagctc gacgaccgca tcgcggcgca ggtggccgag cggatccccg acggcgccac
     2161 cgtgcaggtc gggatcgggt ccatccccaa cgcgatcctc catcgactgg gcgaccaccg
     2221 ggacctgggc ctccacaccg agctgctcag cgacggcgtg atccccctcg tggagtccgg
     2281 cgtgctgaac ggcacccgca agcgccggcg ccccacgaag ctggtcgcca cgttcgcgct
     2341 cggcacgcgt gcgctgtacg acttcatgga cgagaacacc atcgtcgaca tgctcccggt
     2401 ggactgggtg aacgatccgc gcatcatcgg ccaggacgac tgcttcgtgt cggtgaacgc
     2461 cacgaccgag atcgacttcc tcggccaggc ggcgtcggag acgatcggtg gccgctacta
     2521 cagcgggtcc ggcgggcaga ccgacttcgc tcgcggtgcc cagtactcgc gcaacgggca
     2581 gggcttcatc gtgctgcact cgacgaccaa cgacgggacc aagtcgcgga tctcggccca
     2641 gctgccgccc ggctcggtgg tcaccacgtt gaagaacacg atcgacaacg tggtcaccga
     2701 gtggggcgtg gccgagctgc ggggccggtc gctgcgggag cgggccgctg cgctgatccg
     2761 ggtggcgcac cccgacttcc gggaccagct cagccgggag gcccgggagc tgggctacct
     2821 gtagcggccg ggccgccttc agagggtgga ggtgtcggag tcgaggccga acgcgagccg
     2881 gccggcgagc tcgtaggtgc gctcggcggc catggcgtgc tgggacacga cgttggcgtc
     2941 gcggaacagg cgctccagcg ggcagcgctg gtagacggct tcgccgccgg cggcgcggtg
     3001 cagccggctg acgacgtcgg cgcagcgctg cgtggcgtcg gtggtggcca ggcgcacgag
     3061 cctccgctgc tcgatgctgg gctcgtcgcc ggcctcggcc gtggcccagg catccgcgta
     3121 ggcgtcgagc aggaacagcc gggcgctggc gatggtggcc tcggccttgg ccacgtcggc
     3181 ctgggcgctg ggtcgctcgg acagggtgcg gcccgagccc tgggggacct tgccccgcgc
     3241 cagggtcgtg aactcctcga gcgcccgctc ggcgaccccg atggcggtgc atccgatgcc
     3301 gccggcgagc agcgagaaga gcgagaagcg gtagagcggt ccgtccacgt gcgtggtggg
     3361 gcgggtgagc tcgacccagc gtccctccgg cacgaacgcg tcggtgacct cgtagtcggt
     3421 ggagcccgaa ccccggacgc cgagcacgtg ccaggtgtcg tggaacgtga cgtcgtcgcg
     3481 gtcgaggagc acgaacggtg cgaggagccc gtcgtcgcgt ggggcgaccg cgccgtcgtc
     3541 gtcgaccagg agcacgccac ccccgatgac cgtgcagtgc tgggtgcccg acccccactg
     3601 ccagcggccc gtcacccgga gcccgccgtc gaccacgcgg gcccggccca cgggtgcggc
     3661 gaagccgccg gcgaccgccc cgggcggacg gaacagctcc tcggcccagc gcggctcgag
     3721 caacccggcg gtgagcgcgg tggtgttggc gatgaagcag caccacgcca ccgcggtgtc
     3781 gtggcgggcg agctccatga aggtctcgag cccgtccagc agggagatct ccgccccgcc
     3841 gtactccttc gggacccaga accggaagaa gccctcgtcc gtgaggccgt cgaccaggtc
     3901 gtcggggagg cgccgcagcg agtcgatctc gtcgctgcgg tcgcgcagct cgggcccgag
     3961 ggcgcgagcc cgctccgtga acgtgtgctc gcccatccgc ccgccgccgg cctagtggtc
     4021 gtgctcggcg gcgtcggcct cggccttggt ctcgtgcacg gtgccctccg ggtgctccgg
     4081 cggcgtgtag atcgagtaca ggcgcagcgg ctccgacccg gtgttgacca cgttgtgcca
     4141 ggtgccgccg gggatgatcg ccgcccagtc gtcctcgatg tcgcggacct cggacaggtc
     4201 gtccttggcc ggacccatct gcacctgacc cgacccctgc tcgatgcgga tgaactggtc
     4261 gaggtcgtcg tgcttctcga ccccgatctc ctcaccgggc tggatgcaca tcaccgtcat
     4321 ctgcagatgg gcgccggtga acagcaccgt gcggaacgtg tcgttctcga gcgtggcctt
     4381 ctcgatgtcg tcgacccaac cggtcacggg tcctcctcgg gttgcgtggt cctggatgct
     4441 gccactctgc ccgatggcgg gaccgatgac gacccatcgg gaccgtgggt agggtccgca
     4501 ctccgaaccg cccaggggga agaggccatg cgagtcccgc tcaccgtccg agaccacatc
     4561 gaccgtgccg agctcgtcta cggcgaccgg atcgcgttcg tggacgaacc cgaccagccc
     4621 gcgcagtcct ggggctcggt gacgtaccgg gagatggcgg cccgggcgcg ttcgatggcg
     4681 gtcgccttcg accagatggg ggtgaagcac ggcgagcggg tggccatcgt cagccacaac
     4741 tcggcccggc tgctcaccgc cttcttcggg gtcagcgcct acggccgcgt gctcgtgccg
     4801 atcaacttcc ggctcaaccg ggaggagatc gcctacatcg tcgagcactc cggagcgtcg
     4861 gtcctgctga tcgatccggc cctggccgac gacctcgccg gggtcgaggc cgagcacacc
     4921 atggtgctcg gcgccgacac cgaccagcag ctgttccgtg ccgtgggcga gcccatcccg
     4981 tgggagcccg acgaggatgc caccgccacg atcaactaca cgagcggcac cacggcccgg
     5041 cccaagggtg tgcagctcac ccaccgcagc ctctggctga acgccgccac gttcggttgg
     5101 cacatggggg tcagcgaccg ggacgtgtac ctgcacaccc tgcccatgtt ccactgcaac
     5161 ggctggggca tggtctacgc cgtcaccggc atgggcggcc gccacatcgt gctgcgccag
     5221 atcgacggcc cggagatcct ccgccgcatc gacgaccacg gcgtgacgct gctgtgcggc
     5281 gcacccgccg tcgggtcgat cgtgctcgac gcggccagct ccctcggcga cgacgtgccg
     5341 ggccggggca ccacccgctg ggtcatggcc ggtgccccgc cgcccaccgc gaccatcgag
     5401 gcggtggagc gcgacctcgg ctgggagttc gtccagatct acggcctgac cgagacctcg
     5461 ccgctgctca cgatcaaccg cggccgcgcg gagttcgacg acctcgaccc gaccgagcgg
     5521 gcccagaagc tgtcccgtgc cggtgccccc gccatcggcg tgcagctgtc gaccagcgag
     5581 tccggggagg tgctggcccg gtccaacgtc gtgctcgagg gctactggga gcagcccgac
     5641 gccagcgccg ccgccctcga gggtggctgg ttccacaccg gcgacggcgg gttcctcgac
     5701 gacgaccact acctgcacat ctcggaccgc aagaaggacg tgatcatctc cggcggcgag
     5761 aacgtgtcct cgatcgaggt cgaggactgc ttgttctcgc accccgacgt ggctgaggtc
     5821 gccgtgatcg gtgtccccga cgagaagtgg ggcgagatgg tcaaggccct ggtcgtcctc
     5881 gcaccggggg ccaccgccga cgaggccgcg ctcatcgagc actgccgctc gaagctggcc
     5941 cactacaagt gccccaagag catcgacttc cgcgacgagc tggcccgcac cgccaccggc
     6001 aagctccaga agttcaagct gcgtgccccc ttctgggagg gccaggagcg ccaggtgaac
     6061 taggagccgc gcgggtcgct tcctacgatg cgcaggtgcg catccggcga ccaccactgc
     6121 tcctcctggc gctgctcgcc gcgctcgtcg cggtcgcggc gcccgcggcg gcgcacaccc
     6181 agatccagcg ggcgaccccc ggacccggcg agtcggtcag tggcgagctc gacgtcgtgg
     6241 tgctggactt cctcgacccg gtggtgccca cccccgagat caccgtcacc gggccggacg
     6301 gcgcgctcgt ggccggcctg caacccgccg agctcatcgc cgacgacgtg gccgtcgccc
     6361 gcttcgaccc cctgacccag gccggtcagt accaggtgga ctacaccttc gtggccctcg
     6421 acggggacga gcaggtgagc gcgcacacct tcaccttcga acccgaccag ggactggacc
     6481 tcgaggtccg cccgttgctc gcctggatcg tcggcggcgt cctggtcgcg ctcgtcgtcg
     6541 ctgcgctgat cggtcggcgt cgagcgcgcg gctgagccga gccgacagac tcgccggcgt
     6601 gctgccgatc acactcgaca ccgtccggat cttcgtccac gtgctcgccg cgacgatctg
     6661 ggtgggcggc cagctgaccc tcgccggcct gctgccaggg ctgcgcaccc tcggccccga
     6721 tgccccccgc accatcgccc gacgcttcaa ccggatcgcc tggcccgcct acggggtgct
     6781 cgtcgccacg ggcgtgtgga acctgttcga ggtcagcttc ggcgaccgcg acaccgactg
     6841 gcaggtgacc ctgttcgtca agctcgtggt ggtggccctc tccggcctcg ccgcggtgac
     6901 ccactcgatg tcggccagca aggcggtgct cgccatcgga ggcgcgatgg ccggcctgtc
     6961 gagcctcgcc gcgctgttcc tcggggtgca gctcgcgttc gggtgagggg tacgaggggc
     7021 gcgaacgcca ggccacgcaa gcatccgcgc ccgtcagcgt ggcgttggcc agactgcgcc
     7081 ttcgacaaca caccccttcc aggaggaaaa atgaacaaga gcgagctcat cgagcaggtc
     7141 gccaagcagg caggcgtcga caagaagacc gccggcgacg tgctgaaggg cttcgaggag
     7201 gtcgtgctcg cggtgaccaa ggccggcaag gaggagatct cctggaccgg gttcctgaag
     7261 ttctcccagg gcaagcgcaa ggccggcacc gcccggaacc cgcagaccgg cgagagcatc
     7321 aagaccaagg ccaagaagat ccccaaggtc acgctcggcg ccaccttcaa gaaggtcgtg
     7381 aacggcgaat cgcccgcacc caagctggcg aagtgagacg ggccggtcgc tgaccggtcc
     7441 cgccccgctg cactcgactc gggccggccc tcgtggccgg cccgagtcgc gtcccggctt
     7501 cggccgcgga ctggcccggc cggtgcctcg tcggtgacga tgggacggtg cgccgcctcc
     7561 tcgccaccct cgccctcgtc agcgtgctcg ctctcggcgc gctcgccggc gctgcggtgc
     7621 cggtcgcggc ccagaccgac gggcaggtca ccacgaccac ggttccccgg ggcggcgaca
     7681 tcatcccgca gccgaactcg ggctccgagc ccgaggatgc cggcgaccgc ggcggtgcgc
     7741 tccagaccgc cctcttcatc ggcctggtcg gcgtcatctt cgtggccggg gcggtcatcg
     7801 tccgccagag ccgcaaggcg cgcgccgagc gagggttctg agccagtccc tcagtcgacc
     7861 aggcggcggc agggatcggc accctcgacg aagccggccc ggaacgccgc cgtccgctcg
     7921 aacgcgctgg tctcggcctc gccgccgaag gcgaggaagg cgacgatgat ctcgtccagg
     7981 tcgcccggtg agaggacgag gcgctgctcg gggacggtct ggaggaactc ggccgcagcg
     8041 aagaccccgg tgaggcagtc ggcgtgcagg gcgccgacga cctcgtcgtc cggctcgatg
     8101 ccgagctgct cctgggcggc gaaggcgtag aggcgggcga gctccccgcc gaacgcgaag
     8161 tcaccgatct cggccagcgc cgggatgagc tcgtcgccgt ccatgtaggc ggtgtcgtcg
     8221 gcgacgcagt agaaggcggc cagctccagc tcggcctcgc tgatctcctc gtcaccgcag
     8281 gtgatggcgc cgtcggcggg gtcggtggcc tcgaagccgc cgacggggtc ccagtcctcg
     8341 ccctcctctg cgaggagggc ggcgtagaag gactcgaggt cggcgacgag cggctcgtag
     8401 agctcgtcga gggggaggtt cccgccggag gcagcatcgg tcgggctgcc gaacgggatg
     8461 gcgacgaccg ccggcggctc gtcggcgtac gtggcacagg cggcggggcc gtcctcgtag
     8521 ccctgctgga aggcgctgac ccggtcgaac cccgagccgt gggcgctgga gtcgtagccg
     8581 gggacgccca gggtgtcgcg cagctcgagc aggcccgcga ccgcggagtc gagggaatcg
     8641 gcatccgtgt ggaacgtcgg gatccggtcg tcgacgtcgt cgacccaggc gccggcgaag
     8701 cagtcggcct gcagctcgag ggtgacggtg gcgccgcgca ccccggcgcg ggcctggacg
     8761 gcgtgggcga actcatgggc catgaccagg ccgaccgtga gggaaccgaa ccgctcctgc
     8821 agaccgggta tgaggttctc ccggtcccag gcgatgaggt cctcggccgg gcagtacagg
     8881 gcgttctcgg cgatctggtc gtaggtcagg gggtcgggtc cgcaggcggg gagcggcgcg
     8941 cggggcccgt aggggacgta gccgccggcg agggggcgga actcggtgtc gtagacctcg
     9001 accatggccg cggtccagta ggcgcccagg tcgtcgaggg ccagctgggt gacctcgtcg
     9061 ggatcggtga cgacgtcgcc gggggggatg tcgggttcgg gccggaccag gtcgccgccg
     9121 tcccggaggg tcaggtccga ctcggtgcac gccgtcgccg ccgccacgag gacgacggcg
     9181 aggagcaacg gcagcgaccg gcggcgcacc cggtcgacgc taccgccggg tgctcaggcg
     9241 tcctcggcgg tcaacggcgc gcggcgggcg cggcgggcgg gcgccttcgg gcgcagcgca
     9301 gccgcgtaga tctccggggt cgggcggggt gcgggcgcct cgaactcggt ggcggcgggg
     9361 tcctcgcctc ggacggcggc ctcgagctcg cgcagggtct gctggagcat cgcccggtcg
     9421 ctgcgctggt tctgcacgat gatcaggccg atgcccatca cgacgaggga caggccggcg
     9481 atgccgcccg agagcaggta ggggaactgg gcctccaccc ggtcgacgct ggcagcgccg
     9541 ttccagccga ggaacacgag cagcacgccg gcggcgcaga ggccggttcc gagcttgctg
     9601 ccgaggtcgt tccagatctg tctcatcttc agctcccgag gtaggtctcg cgcaggttcg
     9661 cggactggat ctcctcgccc acgccttcgt agacgatggt cccctgctcc atgatcaggg
     9721 cccggtcggc gtaggggagg acgccgacgt tctgctccac gatgacgacc gtcgtgccgt
     9781 cgccgcagat gcgcttcacg acctcgaaca ccacgacggc cagcgccggg gacaagccga
     9841 gggaggcctc gtcgatgagg aggaggcgag gggcggacat gagcgcccga ccgatggcga
     9901 gcatctgctg ctcgccgccc gacagcgtgc ccgcgacctg cgagcggcgc tcggcgaggc
     9961 gggggaagta ctcgaacacc aggtcgcgct gggtgtcgaa gacgcccttg tcgttgcggc
    10021 gcacccagtt gcccatgcgc aggttgtcct cgacggtgag gcgggggaag agccggcgac
    10081 cctcggggga gagggagatg ccggcctgca ccaggtccga aggagagcgc ccaccgatgt
    10141 cctcgccatc gaacacgatg cggccggacc acggggcgcg catcccggcg accgtggtga
    10201 cggtggtggt cttgccggcg ccgttcagcc cgaacaggac ggccgtctcg ccctcctcga
    10261 cggcgaagct gacgccgtgg agcacctcga agtcgtagcc cacgacgagg tcgtggatct
    10321 cgagcagtgc catcacgcca cctccgcatc ggggtccacg ccgaggtagg cgcggaccac
    10381 gtcggggtgg ttgcgcacgt cgtcgggttg gccctcgcag agcacctggc cgaggttgag
    10441 gcagtacatg tagtccgaga tgcgcgtgac gagcgggacg tggtggtcga tgagcacgat
    10501 cgtgaggcgg aagcgctcgc ggatgtcgag caggaaatcg ccgaggtcgt cggcctcctc
    10561 ggggccgagc ccggagcccg gctcgtcgag caggagcagc tccgggtcgg tggccagcac
    10621 cgcggcgatc tcgaggcgct tctggatgcc gtagggcagg ccggcgaccc gggtgtccca
    10681 gaggtggccg agatcgacga gatcgaggat ggcgcgtgcc cgctcggtgg cctcgcgctc
    10741 gacctcgctc gccttcgtgg agccgaacag ggccccgagg gcgccgtagc ggaactggag
    10801 gtgctgagcg gacttgaggt tctctccgac ggtggagccc ttgaccaggc cgacgttctg
    10861 gaaggtgcga cccatcccgc gcgtcgcacg ctcgtggacc gggaggtcgg tgacgtcctc
    10921 acccttgaag cgcacgtgcc cggaggtggc ctcgtagaag ccggtgatgc agttgaacag
    10981 cgtcgtcttg ccggcgccgt tcggtccgat gatgcccacg atctcccagc gatcggcgcg
    11041 caggtcgacg tcctgcagcg cttgcacgcc accgaagcgg atcgacacct gcgatgtctc
    11101 cagcaccgtc tcggcggacg gctcacgctc ggacgtcatt gacggtcacc tcctcgtcgg
    11161 cggggatcca gcggaagcgg cggccggcca accactcctg gaacggccgg gccagctggc
    11221 cgaagccgcc ggggagccgc gtgaggacca cgagcagcag gatcggtccg atcacgagcg
    11281 gtgcgaactc cggtgacagg tcgatgaggt cgagcgggcc ggggatgggt ccgaggccct
    11341 cctcgaggcg cagcttctcg accaggtagc cgttgcgcag gaaggcgaag aacatcgatc
    11401 cgaggacgac gccggtccgg ttgcgcaggc cgcccaccgt ggtcatcagg acgaacacga
    11461 gggcgaggtt gaagtcgaag gggttcgcca cgacctgcag gtctcggtgg gccagcaggc
    11521 cgccgcccag tccggcgaag aggccggcga cggcgaaggc gaagagcgtg tacgccttcg
    11581 tgtcgatgcc ccacgtctgg gcgacgcggg ggttctcccg gagggcgagg atcgcccggc
    11641 cgcccttggt gcgcagcatc cgccagtcga tgaggaggac ggcgacgagg ccgatgaggg
    11701 cgaagtagta gtagcggtgg tcgctctcga accccgtggg gcggggggcg atcttgccgg
    11761 cggtcccgcc ggtgaacgcc tcgatggcga agatcgactg ctgggccatc acgccgtaga
    11821 cgagggtgac gagggcgaag tagagcccgg tgagccgcag cgacacgagc ccgagcagga
    11881 tcgactgcac ggcaccgacc agcgcggcga ccaggacgcc cagcaccacc tcctgggtga
    11941 ggtcggtgac gacgtaggcg gaggtgaagg caccgatgcc cacgaaggcc tggtgtccga
    12001 gggagacctg gccggcgtag ccgatgagca cgttgagcga caggccgatg atggcgaaga
    12061 tgaccgcctc gctcatccgg ttggcgtcga acgccggcac ctggaacggc gacgcgatgt
    12121 tggcggggta caggagcacg agccaggcga ggaacgccca gaccaggccg cgggagaccc
    12181 agccgagcgt gctcggaccg gtgcgggtct cgtccgggtc gtcctcgccg tccggggcgc
    12241 ggaggacgtc gagctggacg gtcgggtcga cggcggtcac gtcgccatcc gtccgccgag
    12301 gaggccttcg gggcgcacca gcagcacgag gagcaggacg acgaagacgc tgacgctgga
    12361 tgccccgggg atcacgtcga aggtcgagag gcgcccgaag gactcgagca tcccgatgac
    12421 catgccgccg agcacggcgc ccggcagcga cgtgagcccg ccgatgacgg cggcggtgaa
    12481 gccggggatg agggccaggc tggtgatggc cccgggggtg aaggcgttgc cgccctgcag
    12541 gaggatcccc gcgaaggccc cgagcgtgcc ggcgatgccc cagaccagca tcgagatgcg
    12601 gttcacgtcg aggccgacca gggccgacgc gtcggcgtcc tggctggcgg ccagcaccgc
    12661 caggccgtag ggcgagcgga agaaggcggt gagggcggcc accgcgacga gcagcaggag
    12721 cagcgtgagc atccgctggc ccgagacctg gacaccggcg acgcgggcgc cctcccactg
    12781 ggtgagcggg tcgaagaacc ggagggcgtt gtcaccggtg cgccagaagg cgacgccgat
    12841 ggagagcagg gcgatgccgg cggtggcgac gagcagggtg accttgggtc cctgggacag
    12901 cggccggatc acgaagcgct ccgtgagcag cgcggcgacg atgccgcaga cgaccccgaa
    12961 cagcgctgcc accacgtagg ggaagcccca gtagctgaag gagaagtagg cgccgtacac
    13021 ggccacggtg ccgaactcgg cctgggcgaa gttgaagatg ccgctcgacc ggtagaccag
    13081 caccaggctc agcgcgatca gggcgtagac ggcaccgttg accgtgccga tcacgagcga
    13141 ctgggtgagc tcggtgccgc tgatgccgag gagcacgatc agaacccgtt cacgaaggtg
    13201 ccgatggtct tgaacgtgcg gctgccgcaa tcggcctgga gcacgtggac cgaacgggcg
    13261 ccgaagtggt tctgggccga gtactgcagc ggcgggtaga cgccggtctc gaacgcggcc
    13321 ccgccctcga gcgcctgcac gaagctctgg cgggtcacgc cctcacccgc ggccaggaac
    13381 atctggtgca cggccttgtt caagccccac agggcgatgg cgaggtcgtc ggggtcgccg
    13441 ccgacgtgct tctggtaggc gggccggaac tgggggtcga gctggtcgat gacgtcgagc
    13501 tgggggaacg gcgagaagaa gatggccttg tcgaccgagt tggcggcgct gcagccgacg
    13561 ttggccacgg tgttgagccc cgaggtcacc cctgggccga tccactgcgg gcggtaggcc
    13621 tgtccggagg aggcggcggc gacgttcagg aacgtcaccg ggctggacag gaagtacacg
    13681 atctcggcac cggagccggc catgtcggcg gcgatgccga gcgcctggtt ctggctggcc
    13741 cccttcggga tccgcttgtt gtagacgacg tcgaggccag cgtcctgggc cgctcgcacg
    13801 atcgacgcgt gggcgtcgtt gaacgtgggc gagtcggcca cggcgatgcc gaacttcccg
    13861 ccacccagct gcttctgggc catctggacg agcttggccg actgctgggc gtaggtctcg
    13921 gagagggcga agtacgcccg cagcccggac aggccctcct cgttcacgcc ggccgacagg
    13981 tagggcaccc cgacgctctg cgcgtactgg gcgcacgccg tgatctggtc agcgccgccg
    14041 ccgcccacga gcaggaagac cttgtcctcg gtgaccatct tcttgcactt ggccaccgcg
    14101 gtgcggggat cgaactggtc gtcctcgaac acgacccgca ccttgcggcc gaacaggccg
    14161 ccctgctcgt tcagggcctg ccagtagaca tccttggcct cgtcgaagga gctctggggg
    14221 aggggagcgg cgccggtgac cggggcgtgg acgccgatca cgatctcggt gtcggtgatg
    14281 ccggtgcggt ccccggccgg gacggcgccg ccaccacctt gatccgcagc cgggtcacca
    14341 ccgcctggtt cgccgccacc gccaccgcca ccggcaccgg tgccgccacc ggccggggtg
    14401 gtggccggcg tcccgccggc ggtcgtgcgc ccggcggtcg aggtcttggc acccccggtc
    14461 gcaccggtgg caccgtcgat gtcgtcgaac gcgccgccgg tgctcccggt ggcgtcgtcg
    14521 aagccgctgc tcgggtctgc gccggcggag acgctggcga cgtcggtctt ctgcccgcac
    14581 gccgcggcga tgagcagcag cgcgacgagc acggccacgg tggccgatcg gcggtgggta
    14641 cgtcggaccg ggttcacggt tgtcagcgct cctgtgcgg
//
LOCUS       DATISU010000262         3413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_388154, whole genome shotgun sequence.
ACCESSION   DATISU010000262 DATISU010000000
VERSION     DATISU010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388154"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VLR27_10445"
     CDS             <1..593
                     /locus_tag="VLR27_10445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HSP03912.1"
                     /translation="KLDKETGELIDTGYQMATADEGIRDTTMEILGNLKPAFLPDGKI
                     TAGNSSQICDGASAVLIISEEKANELGLTPRARFHTFALAGVDPVTMLTGPIPATEKV
                     LAKAGMTMDDIDLFEVNEAFASVVGAWQKEHSADMSKVNVNGGAIALGHPLGGSGAKL
                     MATLLNELERTGGRYGLQTMCEGGGLANATIIERLG"
     gene            complement(689..1099)
                     /locus_tag="VLR27_10450"
     CDS             complement(689..1099)
                     /locus_tag="VLR27_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSP03913.1"
                     /translation="MSQAIASLPVSSLIGDELITVTADTTIDAVARVLDDKGISIVAV
                     GDPSKVEGVVSERDVVRVVARGDDPTTVTAGDVASRELVWCQEDSTVPTVANEMFDRY
                     VRHVLVANPDGLAGIVSARDLLGVYASEDTDWDA"
     gene            1208..1531
                     /locus_tag="VLR27_10455"
     CDS             1208..1531
                     /locus_tag="VLR27_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03914.1"
                     /translation="MPKLNPRWLVIGTLVVVVGAVVSDVSVFSLIIAVLAGALIFKVG
                     FAILGGMAQPIPEPPPAGELRKVKIEYRCTICGTEIRMTVANDEMPDPPRHCLEDMDL
                     VTPVE"
     gene            complement(1625..1960)
                     /locus_tag="VLR27_10460"
     CDS             complement(1625..1960)
                     /locus_tag="VLR27_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03915.1"
                     /translation="MARQDRKKKGDGGRTTPKGGSGSGARHAISKGHAAGEVRESTSG
                     RYTAPIPQEFKVSPTWVPVVMFGLLGLGALVIFLNYLGLLPGATDNLYLLLGLALVLG
                     GIVTSTQFH"
     gene            2005..>3413
                     /locus_tag="VLR27_10465"
     CDS             2005..>3413
                     /locus_tag="VLR27_10465"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HSP03916.1"
                     /translation="MIEQPSERVLAAVDIGTNSVHMVIARVGADGRMEILEREKDMVR
                     LGRSAGDMKELAPDAIDRGVAALKRCRSVADAHGAELAAVATSAVREAENRSEFLDRA
                     WDEAGVSVEVISGFEEARLIHLGVLQSLPLFEDRVLLCDIGGGSTEVLLGSRGDVLAS
                     RSFRLGAIRVTERFLSKDPVKPKHVERCRQHAETILTAFGTEVAEAGFDTVVGASGTI
                     ETVVAMALAARGEGSGLRTLNGQRISADEVTEVVELLAATPDQKERASLPGMDAKRAD
                     ILLGGAVILEQFVRCFGVEELVFSDYALREGVLLDQARRRSGATLHHLQDLRRRSVLH
                     LMELTDEHPEHSEEIARHALLLFDELQPLLELGDEARPLLEAAALLCNVGAFISHSGH
                     HKHSYYVIRNSEHLAGFTDREIELIAQVARYHRKSAPKSKHAEWRSLRRADRDLVERL
                     AAVLRVAIGLDRSHRARVS"
BASE COUNT          514 a         1226 c         1189 g          484 t
ORIGIN      
        1 agaagctcga caaggagacc ggcgagctca tcgacaccgg ctaccagatg gccacggccg
       61 acgagggcat ccgcgacacc acgatggaga tcctcggcaa cctgaagccg gccttcctcc
      121 ccgacggcaa gatcaccgcc ggcaactcct cccagatctg cgacggcgcc tcggccgtgc
      181 tgatcatttc cgaggagaag gcgaacgagc tcggcctcac cccgcgggcc cgcttccaca
      241 ccttcgccct cgccggcgtc gacccggtca ccatgctcac cggccccatc ccggccaccg
      301 agaaggtgct ggccaaggcg ggcatgacga tggacgacat cgacctgttc gaggtcaacg
      361 aggcgttcgc ctccgtcgtc ggcgcctggc agaaggagca cagcgccgac atgagcaagg
      421 tcaacgtgaa cggcggcgcc atcgccctcg gccaccccct cggcggctcc ggcgccaagc
      481 tcatggccac gctgctcaac gagctcgagc gcaccggcgg ccgctacggc ctccagacga
      541 tgtgcgaggg cggcgggctc gccaacgcca ccatcatcga gcgcctcggc tgaacgaatc
      601 tggcagccca gccgtcacac cgcgttgcgt cgagcagcgg ctcaacgctc ctcggtgtga
      661 cggaggggct gccagatccc tctcggggtc aggcgtccca gtcggtgtcc tcggaggcgt
      721 agacgccgag gaggtcgcgg gccgagacga tgccggccag gccgtcgggg ttggcgacca
      781 ggacgtggcg cacgtagcgg tcgaacatct cgttggccac ggtggggacg gtggagtcct
      841 cctggcacca caccagctcg cggctggcga cgtcgccggc ggtgaccgtg gtggggtcgt
      901 caccacgggc gacgacgcgg accacgtcgc gctcggagac caccccttcg accttggagg
      961 ggtcgcccac ggcgacgatg gagatcccct tgtcgtcgag gacccgggcc accgcatcga
     1021 tcgtggtgtc tgcggtgacg gtgatcagct cgtcgccgat gagcgacgag acggggaggg
     1081 atgcgatggc ttggctcatg cctcgatcct ggcccccgga ccgccaccac cgtcagggtc
     1141 gggagtcaca ccccgcgggg atcccgtggt ggcaggccgg cgccggaggg tcggtaccct
     1201 gcgggtgatg ccgaagctga acccacgatg gctggtcatc ggcaccctcg tcgtggtcgt
     1261 gggcgccgtc gtctccgacg tgagcgtctt ctcgctcatc atcgcggtgc tcgccggcgc
     1321 cctcatcttc aaggtcggct tcgccatcct cggcggcatg gcccagccca tccccgagcc
     1381 gccgccggcc ggtgagctgc gcaaggtcaa gatcgagtac cgctgcacga tctgtggcac
     1441 cgagatccgc atgaccgtcg ccaacgacga gatgccggac ccgccccggc actgcctcga
     1501 ggacatggac ctggtcacgc ccgtcgagtg agcaggcgcg cgcccaccct gtggaaaact
     1561 ccgcttccct ggggacagac ctcgcgtcag gctgtggaca accggtggac gaccggtgtc
     1621 gccgtcagtg gaactgcgtg gaggtgacga tgccgccgag gaccagggcc aggccgagca
     1681 gcaggtacag gttgtcggtg gcgccgggca gcagccccag gtagttgagg aagatcacca
     1741 gcgcgcccag gccgagcagg ccgaacatga ccaccggcac ccacgtcggg ctgaccttga
     1801 actcctgggg gatcggggcg gtgtagcgcc ccgaggtgct ctcgcggacc tcgccggccg
     1861 cgtggccctt ggagatggcg tgccgtgcgc cggagccgct gccccccttg ggggtcgtgc
     1921 gaccgccgtc gcccttcttc ttgcgatcct gtcgtgccac ctcggccacg gtagtccccg
     1981 acggcgctgg gtacggtctc gccggtgatc gagcagccgt ccgaacgtgt cctcgccgcg
     2041 gtcgacatcg ggaccaactc ggtgcacatg gtcatcgccc gggtcggggc ggacggccgc
     2101 atggagatcc tcgagcgcga gaaggacatg gtgcgcctcg gccggtcggc gggggacatg
     2161 aaggagctgg cccccgacgc catcgaccgg ggcgtggccg ccctcaagcg ctgccgaagc
     2221 gtggccgacg cccacggggc cgagctcgct gccgtcgcca cctccgcggt gcgcgaggcc
     2281 gagaaccgca gcgagttcct cgaccgggcg tgggacgagg ccggtgtgtc ggtcgaggtg
     2341 atctccggct tcgaggaggc gcgcctcatc cacctcggcg tgctccagtc gctgccgctc
     2401 ttcgaggacc gtgtgctcct gtgcgacatc ggcggcggct ccaccgaggt gctgctgggc
     2461 agccggggcg acgtgctcgc cagccgaagc ttccgtctcg gcgcgatccg ggtcaccgag
     2521 cgcttcctca gcaaggaccc ggtgaagccc aagcacgtgg agcgctgccg ccagcacgcc
     2581 gagaccatcc tcaccgcctt cggcaccgag gtcgccgaag ccgggttcga caccgtcgtc
     2641 ggcgcctcgg gcacgatcga gaccgtggtc gccatggccc tcgccgcccg gggcgagggc
     2701 agcgggctgc gcacgctcaa cggccagcgg atctcggcgg acgaggtcac cgaggtcgtc
     2761 gagctgctgg ccgccacccc ggatcagaag gagcgggcat cgctgcccgg catggatgcc
     2821 aagcgggccg acatcctgct cggcggcgcg gtgatcctgg agcagttcgt gcggtgcttc
     2881 ggggtggagg agctggtctt ctccgactac gccctgcgcg agggcgtgct gctcgaccag
     2941 gcccgccgcc ggtcgggtgc gaccctgcac cacctgcagg acctgcgccg ccggtcggtg
     3001 ctgcacctga tggagctcac ggacgagcac cccgagcact ccgaggagat cgcccgccac
     3061 gccctgctcc tgttcgacga gctccagccc ctgctcgagc tcggcgacga ggcccggccg
     3121 ctcctcgagg cggccgccct gctgtgcaac gtgggggcgt tcatctccca ctcggggcac
     3181 cacaagcaca gctactacgt gatccggaac agcgagcacc tggccgggtt caccgaccgg
     3241 gagatcgagc tcatcgccca ggtcgcccgc taccaccgca agtccgcgcc caagtccaag
     3301 cacgccgagt ggcggtcgct gcgccgcgcc gaccgggacc tggtcgagcg gctggcggcg
     3361 gtgctgcggg tcgccatcgg tctcgaccgc tcccaccggg cccgcgtgtc gga
//
LOCUS       DATISU010000263         7674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_61912, whole genome shotgun sequence.
ACCESSION   DATISU010000263 DATISU010000000
VERSION     DATISU010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61912"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..1935
                     /locus_tag="VLR27_10470"
     CDS             4..1935
                     /locus_tag="VLR27_10470"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HSP03917.1"
                     /translation="MAAETTSYGANNMRVLEGLEAVRKRPGMYIGSTGPNGLHHCVWE
                     VVDNSVDEAMAGHATRIDVTILPDGACRVVDDGRGIPTDMNKQQGMSGVEIALTKLHG
                     GGKFDGEGYKVSGGLHGVGVSVVNALSSKVIVEVDRDGKHHVMEFADGGKPQGKLKVT
                     GDSPRGRTGTTVTFWPDDTIFETTEFTSRTILERLQMMAFLNKGLEIRYQDQREGHDN
                     ERVTYKYAGGIQDFVRHINKTKEALWSKVGYLEGKDEDGSQEVEVAFQWNTGYNRDGI
                     HSFANGISTIEGGTHEEGFKTALTRVVNNYAKDNRPGNVKDLVLTGDDVREGLTAVVS
                     VRLQEPQFEGQTKAKLGNTSMRSLVTKVTNEYLAQWFEENPTEGNKVIKKAYSAAQAR
                     EAAKNARNAIRSKTLLDGAGMPDKLKDCSAKDRSERELFIVEGDSAGGSAVSARDPRT
                     QAILPIRGKILNVERARIDKMLKNTEIQALISAIGAGVGEEFDVEKRRYDKIIAMCDA
                     DVDGSHIRTLLLTFFFRQMKPLVEAGCIYIAQPPLFSTQVGKSKIYLKDDRAQRAFLA
                     EHPNHKKDFQRLKGLGEMDASELEVTTMNPESRTLLQVTVEQAALADEVMSVLMGDDV
                     AARKDFIVTNAKDVKFVDI"
     gene            complement(1985..2680)
                     /locus_tag="VLR27_10475"
     CDS             complement(1985..2680)
                     /locus_tag="VLR27_10475"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSP03918.1"
                     /translation="MTIIQTTTAPLRPVAVDLYRDIHKGIRAELFAVTQRAGNLDPSD
                     HADKAALAAHVHSVVDLLVSHAEHEDGHIQPALELHLPELATQVEDDHERLEARIALL
                     AEQAGDLVEGAADARRNAHRLYVELAAFTSTYLAHQDLEERVIMPSLEHVIGAEAGLA
                     IHQAIVGSIPPQDMAKSLAIMLPAMNIDDRAELLGGMQAGAPAEVFTAVWSLATSVLD
                     VRDLEALAARLGL"
     gene            2850..3317
                     /locus_tag="VLR27_10480"
     CDS             2850..3317
                     /locus_tag="VLR27_10480"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSP03919.1"
                     /translation="MIDEALQAFLERGGAIIVGSVSPDGVPHAQRGWGCSVTGPTTIR
                     LLLDRSDPVLGEHLDARARLAVTSADVRTLRSVQLKGRVTGVEEPGDEDLCRCDAHNE
                     ELFSDIEQTDHFPRVLTARMVPPSYLVATIEVEELYDQTPGPGAGAQVEAGWT"
     gene            3308..4699
                     /locus_tag="VLR27_10485"
     CDS             3308..4699
                     /locus_tag="VLR27_10485"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSP03920.1"
                     /translation="MDVNMERLTLRDLAPCFEGAIPSVIATASADGVPNVTYLSKVRM
                     VDDERVALSNQFFSKTARNLVENPQADVLIIDPRSYDQYRLHLCFERTARHGPVFDRL
                     RIDVDVAAALEGMQDVYRLRAADIYRVTDIQRVPSGRPPVDRSTLPDVRDGLDAGRIA
                     ELSGRLGRCPDLDTVVETAVSGLAELFGYEHSLLLLLDEDGSRLYTIASHGYAAEGVG
                     SEVALGDGLIGLAAARCTPVTVGNVTQVSKYSRTVRRSYEDSGEIAPGREVPVPGLPD
                     SASRCAVPAMVLGELVGVLTVENRRPVAFGPADEAILTVVATILANAIESARAREREA
                     QPSAPAGSAPTTSSPLPPGAGTHVRFFAVDGSVFLDGEYLIKGVAGRLLWALLGHHER
                     EGRTDFTNREVRLDPSLDLPSFRDNFESRLILLKRRLDEREAPVRIDKTGRGRFRLVV
                     SGAVRLDLVDDVP"
     gene            complement(4703..6595)
                     /locus_tag="VLR27_10490"
     CDS             complement(4703..6595)
                     /locus_tag="VLR27_10490"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSP03921.1"
                     /translation="MRALRRHLPRLRRAPGHPLHLLLLFGMLVPLLGATAVGGTAVHD
                     RYEVRTASLALRARAADLTLQTQFVSALAAEETHSNVLALAAGYDEDTNASGGIDLDV
                     TRARIAGARAVVDSDRFAGIRRTLGLDDRLRRLRQRVDVGSADYRAVSTFFGAADIAI
                     EGAWETAMDELNRYADQEALPARLRNRLRSLRGAMTALAVTDDRVDHALALFIATPTA
                     TRTRLLIDASARFDAGLEAAEETASPRLAATLARLDTSPAAQRTEENMDLATRIGLGV
                     DPPLSDIDTATLAGGLADGARWAILLTDVVRAAAVELEEAASVHADWAQRTMVTQLLG
                     MGALIAASLAVGTVMARAITTPVRRLEGAVRQVEAGEFQLDPLPDHGPRELAAMARAF
                     NDMSGTLAAVEDHAVALATDPGSPVLDEALPGRTGEAMQAALDRLRASIAEAEARRAE
                     LVELATHDSLTGLLNRGAAMQALARDLARAERDGSSLLALFLDLDGLKQLNDTHGHDA
                     GDAAIRAAADALRASTRDGDVVARLGGDEFLVVGPLPPGGLADAERFAHRIHDSVRSQ
                     TVRTATGSAVPLRCSVGAAVSGSDTGSIDDLVRAADLALYDAKDAGRDQVAWAPDLAR
                     RISGLR"
     gene            complement(6664..>7674)
                     /locus_tag="VLR27_10495"
     CDS             complement(6664..>7674)
                     /locus_tag="VLR27_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSP03922.1"
                     /translation="ERWGLLLTEVVGAAARDLAEAAGEHAADAGDDVRNEVVLAAVLI
                     VASAAVGLATAATLTRPARDLQSAARRVEAGDFESDPVPVRGPMELRETVLAFNDMAA
                     TLAAVEDHAVALAEDPTSTVLDDPLPGRTGLALQQAIDRLRTSVHQAETHRAELLELA
                     THDGLTGLLNRAAAFTAIQHDLSRADRDGTQLLALYVDLDGLKLLNDTYGHDVGDEAI
                     ARTADALRSTTRDSDVVARLGGDEFMVVGPVPEGGRDEIAAFAERIRAAVSLQTVRVG
                     AGEAAPLRCSIGVALSDEWTGSAEALVRAADEAMYAAKHAGRDRIAWTDTAPDQRDGT
                     PH"
BASE COUNT         1144 a         2692 c         2767 g         1071 t
ORIGIN      
        1 gttttggctg ctgagacgac gtcgtacggc gccaacaaca tgcgggtcct ggagggcctc
       61 gaggccgtcc ggaagcgtcc cggcatgtac atcgggtcca ccggcccgaa cggcctgcac
      121 cactgcgtgt gggaggtcgt cgacaactcc gtcgacgagg ccatggccgg ccacgccacc
      181 cgcatcgacg tcacgatcct gcccgacggt gcgtgccggg tcgtcgacga cggtcgcggc
      241 atccccaccg acatgaacaa gcagcagggc atgtcgggcg tggagatcgc cctcaccaag
      301 ctgcacggcg gcggcaagtt cgacggcgag ggctacaagg tctccggcgg cctccacggc
      361 gtcggcgtct cggtcgtcaa cgccctgtcc agcaaggtca tcgtcgaggt cgaccgcgac
      421 ggcaagcacc acgtcatgga gttcgccgac ggcggcaagc cccagggcaa gctgaaggtc
      481 acgggcgact ccccccgggg ccggacgggc accacggtca cgttctggcc ggacgacacc
      541 atcttcgaga ccaccgagtt cacgtcccgc acgatcctcg agcgcctcca gatgatggcc
      601 ttcctcaaca agggcctgga gatccgctac caggaccagc gcgagggcca cgacaacgag
      661 cgcgtcacct acaagtacgc cggcggcatc caggacttcg tccgccacat caacaagacc
      721 aaggaagccc tctggtccaa ggtcgggtac ctcgagggca aggatgagga cggctcgcaa
      781 gaggtcgagg tcgccttcca gtggaacacc ggctacaacc gcgacggcat ccacagcttc
      841 gccaacggca tctccaccat cgagggcggc acccacgagg agggcttcaa gacggccctc
      901 acccgcgtgg tgaacaacta cgccaaggac aaccggcccg ggaacgtcaa ggacctcgtc
      961 ctcaccggtg acgacgtccg cgagggcctc accgccgtgg tgtccgtgcg cctccaggag
     1021 ccccagttcg agggccagac caaggccaag ctcggcaaca cctcgatgcg ctcgctggtc
     1081 accaaggtca ccaacgagta cctggcccag tggttcgagg agaacccgac cgagggcaac
     1141 aaggtcatca agaaggccta ctcggcggcc caggcccgcg aggcagccaa gaacgcccgc
     1201 aacgccatcc gctccaagac gctgctcgac ggcgccggca tgcccgacaa gctcaaggac
     1261 tgctcggcca aggaccgcag cgagcgcgag ctgttcatcg tcgagggtga ctccgccggc
     1321 ggttcggccg tgtcggcccg ggacccccgc acccaggcga tcctgcccat ccgcggcaag
     1381 atcctcaacg tcgagcgggc ccgcatcgac aagatgctga agaacaccga gatccaggcc
     1441 ctgatctcgg ccatcggggc cggcgtgggc gaggagttcg acgtcgagaa gcgccgctac
     1501 gacaagatca tcgccatgtg cgacgccgac gtcgacggct cccacatccg cacgctgctg
     1561 ctcacgttct tcttccgcca gatgaagccg ctggtcgagg cgggctgcat ctacatcgcc
     1621 cagccgccgc tgttctccac ccaggtgggc aagtccaaga tctacctgaa ggacgatcgg
     1681 gcccagcggg cgttcctggc cgagcacccg aaccacaaga aggacttcca gcgcctgaag
     1741 ggtctggggg agatggacgc ctccgagctg gaggtcacca ccatgaaccc cgagagccgc
     1801 acgctgctgc aggtcacggt cgagcaggcc gcgctggccg acgaggtcat gtccgtgctg
     1861 atgggcgacg acgtcgccgc ccgcaaggac ttcatcgtca ccaacgccaa ggacgtcaag
     1921 ttcgtcgaca tctaggccac ggctgaggca gccggcaagc ggccggctgc ccgccgggcg
     1981 agtgtcacag gccgaggcga gcggccaggg cttcgaggtc gcggacgtcg agcaccgagg
     2041 tcgccaggct ccacacggcc gtgaagacct cggccggggc gccggcctgc atgccgccga
     2101 ggagctcggc gcggtcgtcg atgttcatcg ccgggagcat gatggcgagg ctcttggcca
     2161 tgtcctgcgg agggatcgag cccacgatgg cctggtggat ggccaggccg gcctcggcac
     2221 cgatgacgtg ctcgagcgag ggcatgatca cccgctcctc caggtcctgg tgggccaggt
     2281 aggtgctcgt gaacgcggcc agctcgacgt agaggcggtg ggcgttcctg cgggcgtcgg
     2341 cggcaccctc cacgaggtcg ccggcctgct cggcgagcag cgcgatccgg gcttccaacc
     2401 gctcgtggtc gtcctccacc tgcgtcgcca gctcggggag gtggagctcg agcgccggct
     2461 ggatgtggcc gtcctcgtgc tcggcgtggc tcacgagcag gtcgaccacc gagtggacgt
     2521 gtgcggcgag tgccgccttg tcggcgtggt cgctgggatc gaggttgccg gctcgctggg
     2581 tgacggcgaa gagctcggcg cggatgccct tgtggatgtc ccggtacagg tcgacggcga
     2641 ccgggcggag cggggcggtg gtggtctgga tgatggtcac gttggtctcc tggagggcgg
     2701 tgctcggacg tcccgaggcg gttcccgctc ctggtgccat cttccgggag cgccgagagg
     2761 cgtgctcctc aaaggcccgg catggaatgc taagaacatc cgaaccgcat cggtccggcg
     2821 gccacgacat cgcgtcggag gtggagacgg tgatcgatga ggccctccag gcgttcttgg
     2881 agcggggtgg cgcgatcatc gtcgggtcgg tctcgcccga tggggttccg cacgcgcagc
     2941 gcgggtgggg gtgctcggtg accgggccca ccaccatccg gctgctgctc gaccgctccg
     3001 atccggtgct cggcgagcac ctcgacgccc gggcgcgact cgccgtcacg agcgcggacg
     3061 tgcgcacgtt gcggtcggtg cagctgaagg gtcgggtgac cggcgtggag gagcccggcg
     3121 acgaggacct gtgccgctgc gacgcccaca acgaggagct cttctccgac atcgagcaga
     3181 ccgatcactt cccccgggtg ctcacggctc gcatggtccc cccgagctac ctggtcgcca
     3241 cgatcgaggt cgaggagctg tacgaccaga cacccggtcc gggcgccggc gcccaggtgg
     3301 aggcggggtg gacgtgaaca tggagcggct gaccctccgc gacctcgcgc cctgcttcga
     3361 gggtgccatc ccctcggtca tcgccaccgc ctccgccgac ggggtcccca acgtcaccta
     3421 cctgtccaag gtgcgcatgg tcgacgacga gcgggtcgcg ctctcgaacc agttcttctc
     3481 caagacggcc cgcaacctgg tggagaaccc gcaagccgat gtcctcatca tcgacccccg
     3541 cagctacgac cagtaccggc tgcacctgtg cttcgagcgg accgcgcggc acggtccggt
     3601 gttcgatcgg ctccggatcg acgtggatgt ggcggcggcg ctcgagggga tgcaggacgt
     3661 gtaccggctg cgggcggcgg acatctaccg ggtgaccgac atccagcggg tcccatcggg
     3721 acggccaccg gtggatcggt ccaccctccc cgacgtccgg gacgggttgg atgccggtcg
     3781 gatcgccgag ctcagcggcc ggctcggccg gtgccccgac ctcgacacgg tggtggagac
     3841 cgccgtgtcc gggctggccg agctcttcgg gtacgagcac tcgctcctgc tcctgctcga
     3901 cgaggacggt tcccggctct acaccatcgc cagccacggc tacgcggccg agggggtggg
     3961 ctcggaggtc gcgctcggcg acggcttgat cggcctggcc gcggcgcgct gcacccccgt
     4021 caccgtcggc aacgtcacgc aggtcagcaa gtactcccgg acggtgcggc ggtcctacga
     4081 ggacagcggc gagatcgccc ccggtcgaga ggtgcccgtg cccggcctcc ccgattcggc
     4141 gagccgctgc gcggtgccgg cgatggtgct gggcgagctc gtcggcgtgc tcaccgtgga
     4201 gaaccgccgc ccggtggcgt tcggccccgc tgacgaggcg atcctgaccg ttgtcgccac
     4261 gatcctcgcc aacgcgatcg agtcggcgcg ggcacgcgag cgagaggcgc agccgtccgc
     4321 gccggccgga tccgcaccga ccacctcgtc acccctgccg ccgggggcag gcacccacgt
     4381 gcggttcttc gcggtcgacg gcagcgtctt cctggacggc gagtacctca tcaagggcgt
     4441 ggccgggcgc ctgctgtggg cgttgctggg ccaccacgag cgcgagggtc gcaccgactt
     4501 cacgaaccgg gaggtgcgtc tcgatccgag cctcgacctc ccgtcgttcc gggacaactt
     4561 cgagagccgc ttgatcctcc tgaagcgacg tctcgacgag cgggaggcgc cggtgcggat
     4621 cgacaagacc ggccggggcc ggttccgcct cgtcgtgtcg ggggcggtcc ggctcgacct
     4681 ggtggacgac gtgccctgag ggtcagcgca gcccgctgat ccgtcgtgcg aggtctggcg
     4741 cccacgccac ctggtcccga ccggcgtcct tggcgtcgta gagggccagg tcggcggcgc
     4801 gcaccaggtc gtcgatgctc ccggtgtccg agcccgacac ggccgcgccg acgctgcagc
     4861 gcagcggcac cgcggacccg gtggccgtcc gcacggtctg gctccgcacc gagtcgtgga
     4921 tccggtgggc gaagcgctcg gcgtcggcga ggccacccgg ggggagcggg ccgacgacga
     4981 ggaactcgtc acccccgagc cgggcgacca cgtcaccgtc cctggtcgat gcccggaggg
     5041 cgtcggccgc ggcgcggatg gccgcgtcac ccgcgtcgtg accatgggtg tcgttgagct
     5101 gcttgaggcc atcgagatcg aggaagaggg cgagcagcga cgatccgtcc cgctcggccc
     5161 gagcgaggtc ccgcgccagg gcctgcatcg ccgcaccccg gttcaacagg ccggtgaggc
     5221 tgtcgtgcgt cgccagctcg accagctctg cccggcgggc ctccgcctcc gcgatcgatg
     5281 cccgcagccg gtcgagtgcc gcctgcatgg cctcccccgt ccgccccggc agcgcctcgt
     5341 cgaggaccgg tgacccggga tctgtggcga gcgccacggc gtggtcctcg accgcggcga
     5401 gggtgccgga catgtcgttg aacgcccggg ccatggcggc cagctcacgt ggcccgtgat
     5461 cgggcagggg gtccagctgg aactcgccgg cctcgacctg gcggaccgcc ccctcgagcc
     5521 gccggaccgg cgtcgtgatc gcgcgagcca tgaccgtgcc cacggcgagc gaggccgcga
     5581 tgagcgcacc catcccgagc agctgggtca ccatggtgcg ctgggcccag tcggcgtgga
     5641 cggaagcggc ttcttcgagc tccacggccg cggcccggac gacatcggtg agcaggatcg
     5701 cccagcgggc gccgtcggcc agacctccgg cgagggtcgc tgtgtcgatg tccgacagcg
     5761 ggggatcgac gccgaggccg atgcgggtgg ccagatccat gttctcctcg gtccgctgcg
     5821 cggcggggga ggtgtccaac cgagcgaggg tggcggcaag gcgggggctg gcggtctcct
     5881 ccgcggcctc gaggccggcg tcgaaccgcg ccgacgcgtc gatgagcagc cgggtgcgcg
     5941 tcgcggtggg cgtggcgatg aacagggcga gcgcgtggtc gacccggtca tcggtgacgg
     6001 cgagggcggt catggcccca cggaggctgc gcaaccggtt gcgcagccgt gccggcaggg
     6061 cttcctggtc ggcgtaccgg ttgagctcgt ccatggcggt ctcccaggcg ccctcgatgg
     6121 cgatgtcggc ggcgccgaag aaggtgctga ccgcgcggta gtcggcggac ccgacgtcga
     6181 cccgttggcg cagccggcgg aggcggtcgt cgaggccgag ggtcctgcgg atgccggcga
     6241 accggtcgct gtccaccacc gctctggccc cggcgatccg ggcacgggtg acgtcgaggt
     6301 cgatgcctcc ggaggcgttg gtgtcctcgt cgtagccggc cgccagggcc aggacgttgc
     6361 tgtgggtctc ctccgcggcg agcgccgaca cgaactgggt ctgcagggtc agatcagccg
     6421 cccgggcccg cagcgccagc gacgccgttc gcacctcgta gcgatcgtgc accgccgtgc
     6481 cgccgacggc cgtggccccg aggagcggca ccagcatccc gaacagcagg agcaggtgga
     6541 gggggtgccc gggggcgcgc cggaggcggg ggagatgccg tcgcagcgcg cgcatgcccc
     6601 ttcatcggac gaaccggacc tccagaacag agcacaatgg cccgaccttc ccgtcaatcc
     6661 gcgtcagtgc ggggtgccgt cccgctggtc cggcgcggtg tccgtccacg cgatgcggtc
     6721 ccgtccggcg tgcttggcgg cgtacatggc ctcgtccgca gcgcggacca gcgcttcggc
     6781 cgaaccggtc cactcgtcgg acaacgccac accgatgctg catcgcagcg gcgccgcctc
     6841 gccggcgccc acccgcaccg tctgcaggga gaccgcggcg cggatgcgct cggcgaaggc
     6901 ggcgatctcg tcccggccgc cctcgggcac cggtcccacg accatgaact cgtcgccgcc
     6961 gaggcgagcc acgacatcgc tgtcgcgggt ggtgctgcgc agggcatcgg ccgtgcgggc
     7021 gatggcctcg tcgccgacat cgtggccgta ggtgtcgttg agcagcttca gcccgtcgag
     7081 atcgacgtag agcgccagca gctgcgtgcc gtcacggtcg gcgcgtgaga ggtcgtgctg
     7141 gatcgcggtg aaggcggcgg cccggttgag caggccggtc aggccatcgt gggtcgccag
     7201 ctcgagcagc tcggcgcggt gcgtctcggc ctggtgcacc gaggtgcgca gccggtcgat
     7261 cgcctgctgc agggcgagac cggtgcgacc gggcagcgga tcgtcgagca ccgtcgaggt
     7321 cggatcctcg gcgagggcca cggcatggtc ctccacggca gcgagggtgg ccgccatgtc
     7381 gttgaaggcc aacacggtct cgcgcagctc catcgggcct cggaccggca ccgggtcgga
     7441 ctcgaagtcc ccggcctcga cccggcgggc ggcggattgc agatcccgag ccggccgggt
     7501 gagcgtggcc gccgtggcca gcccgaccgc ggcagacgcc acgatgagga cagcggcgag
     7561 caccacctcg ttgcgcacgt cgtcgccggc gtcggcggcg tgctcgcccg cagcctctgc
     7621 gaggtcccgt gctgcggcac cgaccacctc ggtcaggagc aggccccacc gctc
//
LOCUS       DATISU010000264         3202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_201992, whole genome shotgun sequence.
ACCESSION   DATISU010000264 DATISU010000000
VERSION     DATISU010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201992"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VLR27_10500"
     CDS             complement(<1..258)
                     /locus_tag="VLR27_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSP03923.1"
                     /translation="MSDHTTAGDEYRDAWQAAYDQARLRDTAFSTMSGVEVDPVYGEG
                     PYPGQYPYTRGIHASMYRSRLWTMRMFAGFGTAEDTNVRFKE"
     gene            complement(377..2287)
                     /locus_tag="VLR27_10505"
     CDS             complement(377..2287)
                     /locus_tag="VLR27_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HSP03924.1"
                     /translation="MQHPGTDWITDAPDDGTGDVERAARGFVAGHPTGVIERDGRPIW
                     DTGRYDFLRDSPTAPDSVHPSLWRQARLNCVHGLFEVQAPDADGRGGVWQARGYDLSN
                     ITFIAGDTGWIVIDPLTAAETAAAGLALANEHLGERPVVAVIYTHSHIDHFGGVEGVT
                     SAEDVAAGRCQVLAPAGFLQEAVSENVIAGPAMVRRAMYMYGPLLPVGPEGQVDAGLG
                     KATPLGTVGLIPPTEEIHETGAERVIDGVRIVFQLTPGTEAPAEMNFHFPDLRLLCMA
                     ENCTHTMHNLYTLRGAQVRDSLAWSRYINESIDLFADVTDTCFASHHWPRFGADDVRA
                     FLVQQRDLYRWLHDQTMRLANHGLTAAEIAEQLDRESFADLDDLAGEHHVRGYYGTVN
                     HNVKAVYQRYLGWFDGNPANLHPLPPVETATRTVEWMGGPDAVLDKARELLAAAAEHE
                     DLRWLAQVLNQVVFAAPDRADARELQAEVLQRLGFASESAPWRNFYLTGAQELRNGTF
                     DLGDASLGGFAPGMTTSMLFDALGVRLRPEALAGFGCTIEWTLTDRDEVHTVGISNRT
                     LFHSPDRPAPDADARVTTTAPVLVSLIDGALDVEAATADGSLTVEGDTGALTTIAGAV
                     DTFARIFPIVTP"
     gene            2543..>3202
                     /locus_tag="VLR27_10510"
     CDS             2543..>3202
                     /locus_tag="VLR27_10510"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HSP03925.1"
                     /translation="MLAVAPFVGGDDDAVDVTTASAATESGTDVVRTASLGSPSEVRE
                     LLSETTTTLVEATGATTSTTGVPATSTTSTAAAPTEDENEGDDEVAAATTTTTEDTSA
                     RAQQVESSTTSAAPTTTAAPARKTTPTTAAPRPAPTTTAAPTTTTTAPPAPKESSSEG
                     YAYDDPRSTQVWYDLAECESGGNWSINTGNGYYGGLQFSLPTWESVGGPGYPHEHPAA
                     TQ"
BASE COUNT          437 a         1139 c         1182 g          444 t
ORIGIN      
        1 ctccttgaag cggacgttgg tgtcctcggc cgtgccgaac ccggcgaaca tgcgcatggt
       61 ccacagccgg gaccggtaca tcgaggcgtg gatgccccgc gtgtaggggt actggccggg
      121 gtaggggccc tcgccgtaca ccgggtcgac ctcgacgccc gacatcgtcg agaaggccgt
      181 gtcccgcagg cgggcctggt cgtaggccgc ctgccaggcg tcccggtact cgtcgccggc
      241 agtcgtgtgg tcgctcatcg aaggtcccct cggttgctcg gggaccagcc tacggggacc
      301 gacctcccgc tccggtggtc acgatcggac cggatgcggt cgctcccgga ccggcgaagc
      361 ggctgctggt tccgcgtcac ggggtgacga tcgggaagat ccgggcgaag gtgtcgaccg
      421 cgccggcgat ggtggtcagg gcgccggtgt ccccctcgac ggtgaggctg ccgtccgcgg
      481 tggctgcctc cacgtcgagc gctccgtcga tgagcgagac gaggaccggg gcggtggtgg
      541 tgacccgggc gtcggcatcc ggcgccgggc ggtcggggga gtggaacagg gtccggttgg
      601 agatgccgac ggtgtggacc tcgtcgcggt cggtgagcgt ccactcgatg gtgcagccga
      661 agccggcgag cgcctccggg cgcaggcgga cgccgagggc gtcgaacagc atcgaggtgg
      721 tcatgccggg ggcgaacccg ccgagcgacg cgtcgcccag gtcgaacgtg ccgttgcgca
      781 gctcctgggc gccggtcagg tagaagttcc gccacggcgc cgactcggag gcgaagccca
      841 gtcgctggag cacctcggcc tgcagctcgc gggcgtcggc gcggtcgggg gcggcgaaca
      901 ccacctggtt cagcacctgg gccagccagc gcaggtcctc gtgttccgca gccgctgcca
      961 gaagctccct ggccttgtcc aggacggcgt cggggccgcc catccactcc acggtgcggg
     1021 tggcggtctc caccgggggg agcgggtgca ggttggccgg gttgccgtcg aaccacccca
     1081 ggtagcgctg gtacaccgcc ttcacgttgt ggttcaccgt gccgtagtag ccccggacgt
     1141 ggtgctcgcc ggcgaggtcg tcgaggtcgg cgaaggactc ccggtcgagc tgctcggcga
     1201 tctcggcggc tgtcaggccg tggttggcga ggcgcatggt ctggtcgtgc agccagcggt
     1261 acaggtcgcg ctgctggacg aggaacgccc ggacgtcgtc ggcgccgaat cggggccagt
     1321 ggtggctcgc gaagcacgtg tcggtgacgt cggcgaacag gtcgatcgac tcgttgatgt
     1381 agcggctcca cgccagcgag tcccgcacct gggcgccgcg gagcgtgtac aggttgtgca
     1441 tcgtgtgcgt gcagttctcg gccatgcaca gcaggcgcag gtcggggaag tggaagttca
     1501 tctccgccgg cgcctcggtg ccgggcgtga gctggaacac gatgcgcaca ccgtcgatca
     1561 cccgctccgc gcccgtctcg tggatctcct cggtcggggg gatgagcccg acggtgccga
     1621 gcggggtcgc cttgcccagg ccggcgtcca cctggccctc gggtccgacc ggcagcagcg
     1681 gtccgtacat gtacatggcc cggcggacca tggccggccc ggcgatgacg ttctcgctca
     1741 ccgcctcctg caggaacccg gccggggcca ggacctggca ccgaccggcg gcgacgtcct
     1801 cggccgacgt cacgccctcg acgccgccga agtgatcgat gtgggagtgg gtgtagatca
     1861 ccgccaccac cgggcgctcg ccgaggtgct cgttggccag ggccaggccg gcggccgcgg
     1921 tctcggcggc cgtgagcggg tcgatgacga tccagccggt gtcgccggcg atgaaggtga
     1981 tgttggacag gtcgtagccc cgggcctgcc acacgccgcc ccgcccgtcg gcgtcggggg
     2041 cctggacctc gaacaggccg tgcacgcagt tgagccgggc ctggcgccac aggctcgggt
     2101 gcaccgagtc gggggcggtg ggggagtccc ggaggaagtc gtagcggccc gtgtcccaga
     2161 tcggtcggcc gtcgcgctcg atgaccccgg tcgggtggcc ggcgacgaag ccccgggcgg
     2221 cgcgctcgac gtcgccggtg ccgtcgtcgg gtgcgtcggt gatccagtcc gtaccggggt
     2281 gctgcatgcc ggcatggtgg catccgggcg gcgttggacg cgctcgtgca cgggttcacg
     2341 gagttgttgc gaatccatga cgacacctag tgttgcgaac tcgtgacgga accgccccca
     2401 ggcgatcctc cggcctcgcg ccggcaccga ccctgtcccc gaccccctcg gacccatcgc
     2461 acgccacctc gcccccagcc cgcctgacga tcgcaccttc gctcgtcggc ggctggcctc
     2521 cctcgccggt gtcctcgccc tggtgctcgc cgtcgccccg ttcgtgggcg gcgacgacga
     2581 cgccgtggac gtgaccaccg cgagcgccgc gaccgagtcg ggcaccgacg tcgtccggac
     2641 ggcgtccctc ggctcgccga gcgaggtgcg cgagctgctg tcggagacga ccaccacgct
     2701 ggtggaggcc acgggcgcca cgacctccac gaccggggtc ccggccacca gcaccacctc
     2761 gaccgccgcg gcaccgaccg aggacgagaa cgagggcgac gacgaggtcg ctgcggccac
     2821 gacgaccacc accgaggaca cgtcggcgcg ggcccagcag gtcgagtcct ccaccacgtc
     2881 ggccgccccg accaccacgg cggcaccggc acgcaagacg acaccgacca ccgctgcacc
     2941 ccgcccggcc ccgaccacca cggccgcccc gaccacgacc accacggcgc ctccggcccc
     3001 gaaggagtcg tccagcgagg gctacgccta cgacgatccc cgcagcaccc aggtctggta
     3061 cgacctggcc gagtgcgagt ccggggggaa ctggtcgatc aacaccggca acggctacta
     3121 cggcggcctg cagttctcgc tgccgacctg ggagtcggtc ggtgggcccg gctacccgca
     3181 cgagcacccg gccgcgaccc ag
//
LOCUS       DATISU010000265         5032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_854489, whole genome shotgun sequence.
ACCESSION   DATISU010000265 DATISU010000000
VERSION     DATISU010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854489"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VLR27_10515"
     CDS             <1..481
                     /locus_tag="VLR27_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03926.1"
                     /translation="TDPAGDVTLTHISGVELPLDDWLTTFHLGVVVLDPFEYESAWLL
                     DTAGRILDVFSEADVRVGFVVTSTPADAKTFVGPWADELFVLCDPDKAFVKSLQLEQL
                     PAFVHIGIDGTLLGSAEGWDPLEWRAVADNLARIMSWKAPDIPAAGDPRPYQGAPAV"
     gene            553..975
                     /locus_tag="VLR27_10520"
     CDS             553..975
                     /locus_tag="VLR27_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03927.1"
                     /translation="MAKPQQAELRRSGKVPALDPDASVAKLSAQDTPGTSSSEGSIPE
                     DQRPGHHPDQEQDKPDMDKFAERLGIVSEEEEPPEAPHVEEDSKVTRLQPKAEAKAKK
                     AEAKAKADKPSFSPIGLALVGPVTGFLIAKKIIKRLRR"
     gene            1139..2782
                     /locus_tag="VLR27_10525"
     CDS             1139..2782
                     /locus_tag="VLR27_10525"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HSP03928.1"
                     /translation="MTEFEYTPLLPTTGDDTSFRRLDIEGVEVVDLDGEQFLRVAPEA
                     LTALAAEAMHDIAHYLRPSHLQQLRNILDDPEASANDRFVATDLLKNANVSAGGILPM
                     CQDTGTAIIKGKRGGRVLTAGGDEEALARGVFDTYQKDNLRYSQMAPLSMWEEANTKT
                     NLPAEIKISAVDGMAYDFLFMAKGGGSANKSYLYQETKALLSPDRLLSFLDEKLRSLG
                     TAACPPYHLAVVIGGTSAEMALETAKLASARYLDTLPKTGSALGHAYRDVEFEAEVLK
                     LSQEFGIGAQFGGKYFCHDVRIIRMPRHGASLPVAVAVSCSADRQARARITADGVFLE
                     DLERNPARFLPEPTEADLTADVVRIDLDQPMDAILAELSKHPVKTRLSLTGTVVVARD
                     IAHAKISERLQAGEPMPQYLKDHPVYYAGPAKTPEGYASGSFGPTTAGRMDSYVEEFQ
                     AAGGSMVMLAKGNRSKQVTDACARHGGFYLGSIGGPAARLAQDNIRKVEVLEYPELGM
                     EAVWRIEVEDFPAFIIVDDKGNDFFESVSRPIDLKTKRP"
     gene            2796..3692
                     /locus_tag="VLR27_10530"
     CDS             2796..3692
                     /locus_tag="VLR27_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSP03929.1"
                     /translation="MPAALLVVAAAVLWGTTGTAQALGPDGVDPLVVGWVRMAVGGAG
                     LVALASLRRSTASVLPRAWTLAAVASVATYQLTFFGGVRLAGVALGTAVGIGSAPIWG
                     GLVDWRFARWKPSPRWIVAAAIAIAGAVLVAGEPGDAERPGLGLLLAVAAGASYAMYA
                     YALQQLARAGDADRVAATVFVAAAVVLAPVALVAGGGEALGQLTTGRGALMALHLGLV
                     ATTLAYALFTRGVRDTPVATATLLSLAEPVTAVVLGITLVGEELTATSAVGIGLLLVG
                     VTTAATVRTPDPTSGTATDVPV"
     gene            3702..4607
                     /locus_tag="VLR27_10535"
     CDS             3702..4607
                     /locus_tag="VLR27_10535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HSP03930.1"
                     /translation="MRIGIFGGLAATGTIDDVVADVQASEEQGFHSYWVPQIFGYDAL
                     TLLAVVGRETERIELGTSVIPTYPRHPAMLAAQALTTQQACNGRLTLGIGLSHQPVIE
                     HMFGMSFEKPVRHLREYLDVLMPLVSGERVEAHGETISANLALEIPGAQPVPVQVAAL
                     GPQMLKLAGARTAGTITWMTGPRTIADHTAPTIRAAAEDAGNPDPFVTSGVPVCVTDD
                     VEGTRARAAQVYAIYGQLPSYRAMLDREGLEGPADLAIIGDEDTVAGRIRDFADAGVT
                     DLAASEFGSSSDERTRTRELLRSLL"
     gene            4604..>5032
                     /locus_tag="VLR27_10540"
     CDS             4604..>5032
                     /locus_tag="VLR27_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSP03931.1"
                     /translation="MKVRVGYGLGTRTITNDAERFAALVDALESLRFDSLWLSEKITD
                     DCPDPVVALSFAAARTAKLKLGTSVMVLPGRNPMLLAKELASLDRLSGGRLLPAFGLG
                     QVDPAEQQAFGVERTERAKRFDEALPLLRRFWTEDAVDHDG"
BASE COUNT          723 a         1975 c         1650 g          684 t
ORIGIN      
        1 gaccgacccc gctggcgacg tcaccctgac ccacatctcc ggcgtcgagc tccccctcga
       61 cgactggctg acgacgttcc acctcggcgt ggtcgtgctc gaccccttcg agtacgagag
      121 cgcgtggctg ctggacaccg ccggccgcat cctcgacgtg ttctccgagg ccgacgtccg
      181 cgtcggcttc gtggtcacct ccacgccggc cgacgccaag accttcgttg gcccctgggc
      241 cgacgagctc ttcgtgctct gcgatccgga caaggccttc gtcaagtccc tccagctcga
      301 gcagctgccc gcgttcgtcc acatcggcat cgacggcacc ctcctcgggt cggccgaggg
      361 ctgggacccg ctggagtggc gagccgtggc cgacaacctg gcgcggatca tgtcgtggaa
      421 ggcgccggac atccccgccg ccggcgatcc caggccgtac cagggcgcgc ccgccgtctg
      481 actccgggcc ccgatccggg gcctccgtca catccggcat ggccgtgcgg gacctcgggt
      541 acgtaccgca ccatggccaa gccccagcaa gccgagctcc gtcgttccgg caaggtcccc
      601 gcactggacc ccgacgcctc cgtcgccaag ctgtccgccc aggacacccc cggcacctcc
      661 agctccgagg gatcgatccc cgaggaccag cgccccgggc accacccgga ccaggagcag
      721 gacaagccgg acatggacaa gttcgccgag cgcctcggca tcgtctccga ggaggaggag
      781 cccccggagg cacctcacgt cgaggaggac tccaaggtga cccggctcca gccgaaggcc
      841 gaggccaagg ccaagaaggc cgaggcgaag gcgaaggcag acaagcccag cttctcgccc
      901 atcggcctcg ccctggtcgg tcccgtcacc gggttcctca tcgccaagaa gatcatcaag
      961 cggctccgcc gctgagcacg gacaactgac ccacggaccg gcgccagccg gtccggagaa
     1021 cggccggccg gacccggaac gcccgccccc gggttcggtc ggctcgctcg cgcccgggct
     1081 gcgggctcgc tcgcgcctgg gctgcgacgg ctcggcgccg atctgcgatg ctcggggggt
     1141 gacggagttc gagtacacgc ccctgttgcc cacgaccgga gacgacacct cgttccgacg
     1201 cctcgacatc gaaggcgtgg aggtcgtcga tctcgacggc gagcagttcc tgcgggtggc
     1261 gcccgaggcg ctcaccgccc tggctgccga ggcgatgcac gacatcgccc actacctgcg
     1321 ccccagccac ctccagcagc tgcggaacat cctcgatgac cccgaggcct cggccaacga
     1381 ccgcttcgtg gccaccgacc tgctgaagaa cgccaacgtg tccgccggtg ggatcctgcc
     1441 catgtgccag gacaccggca cggcgatcat caagggcaag cggggcggtc gggtgctcac
     1501 cgccggcggc gacgaggagg ccctcgcccg cggcgtgttc gacacgtacc agaaggacaa
     1561 cctgcgctac tcccagatgg caccgctgtc gatgtgggag gaggccaaca ccaagaccaa
     1621 cctgccggcc gagatcaaga tcagcgccgt cgacgggatg gcctacgact tcctcttcat
     1681 ggccaagggc ggcggctcgg ccaacaagag ctacctgtac caggagacca aggcgctgct
     1741 gtcgccggac cggctgctgt cgttcctcga cgagaagctg cgcagcctcg gcaccgcggc
     1801 ctgcccgccc taccacctcg ctgtggtcat cggcggcacc tcggctgaga tggcgctcga
     1861 gaccgccaag ctggccagcg cccgctacct cgacaccctg cccaagaccg gctccgccct
     1921 cggccacgcc taccgcgacg tcgagttcga ggccgaggtc ctgaagctgt cccaggagtt
     1981 cggcatcggt gcccagttcg ggggcaagta cttctgccac gacgtccgaa tcatccgcat
     2041 gccccgccac ggcgcctccc tgccggtcgc cgtcgcggtg tcctgctccg ctgaccgcca
     2101 ggcccgggcc cgcatcaccg ccgacggcgt cttcctcgag gacctcgagc gcaacccggc
     2161 ccgcttcctc cccgaaccga cggaggccga cctcaccgcc gacgtcgtgc gcatcgacct
     2221 ggaccagccg atggacgcca tcctcgccga gctgagcaag cacccggtga agacccgcct
     2281 ctcgctcacc ggcaccgtcg tcgtggcccg agacatcgcc cacgccaaga tcagcgagcg
     2341 gttgcaggcc ggcgagccca tgccgcagta cctcaaggac caccccgtgt actacgccgg
     2401 cccggccaag acccccgagg ggtacgcgtc gggctcgttc ggcccgacca cggccggacg
     2461 catggactcc tacgtcgagg agttccaggc cgccggcggc tccatggtga tgctggccaa
     2521 gggcaaccgc tccaagcagg tcaccgacgc ctgcgcccgc cacggtggct tctacctcgg
     2581 ctccatcggc ggacccgccg cccggctcgc ccaggacaac atccgcaagg tcgaggtgct
     2641 cgagtacccc gagctcggca tggaggcggt ctggcgcatc gaggtcgagg acttcccggc
     2701 gttcatcatc gtggacgaca agggcaacga cttcttcgaa tcggtcagcc gcccgatcga
     2761 tctcaagacc aagcgcccct agcgtcaccg ccaccgtgcc cgccgcgctg ctcgtcgtcg
     2821 cggccgcggt cctgtggggc accacgggga cggcccaggc gctgggcccc gacggcgtcg
     2881 accccctcgt cgtcggctgg gtgcgcatgg cggtcggcgg tgccggcctg gtcgcgctcg
     2941 cctcgctgcg gcgctcgacc gcatcggtcc tgccccgggc ctggacgctg gcggcggtgg
     3001 cctcggtggc gacctaccag ctcacgttct tcggcggggt ccgcctcgcc ggcgtcgccc
     3061 tcggcacggc cgtcggcatc gggtcggcac cgatctgggg cgggctcgtg gactggcgct
     3121 tcgcccggtg gaagccatcc ccccgctgga tcgtcgccgc ggccatcgcc atcgccggcg
     3181 cggtgctggt cgccggcgaa cccggcgatg ccgagcggcc cggcctcggg ctcctgctgg
     3241 ccgtcgccgc cggcgccagc tacgccatgt acgcctacgc gctgcagcag ctggctcggg
     3301 ccggcgacgc cgaccgggtc gcggccacgg tcttcgtcgc ggccgcggtg gtgctggcgc
     3361 cagtggcgct ggtcgccggc ggtggcgagg cgctcggcca gctgaccacc ggccgcggcg
     3421 cgctgatggc gctgcacctc ggcctggtcg ccaccacgct cgcctacgcg ctgttcaccc
     3481 gtggcgttcg cgacaccccg gtggccaccg ccacgctgct gtccctggcc gagccggtga
     3541 ccgcggtggt cctcggcatc accctggtcg gcgaggagct cacagccacc tcggcggtgg
     3601 gcatcggtct gctgctcgtg ggcgtcacca ccgcagcgac ggtccgcacc ccggacccca
     3661 cctcgggcac cgccaccgac gtccccgtct aggttcgcgc catgcgcatc gggatcttcg
     3721 ggggcctcgc cgccaccggc accatcgacg acgtcgtcgc cgacgtccag gcctccgagg
     3781 agcagggctt ccactcgtac tgggtgccgc agatcttcgg ctacgacgcg ctcacgctgc
     3841 tggccgtggt cggccgggag acagagcgca tcgagctcgg cacgtcggtg atcccgacct
     3901 acccgcgcca cccggcgatg ctggccgccc aggccctcac cacccagcag gcgtgcaacg
     3961 gccggctcac gctgggcatc gggctgtcgc accaacccgt catcgagcac atgttcggca
     4021 tgagcttcga gaagccggtg cgccacctgc gggagtacct ggacgtgctc atgcccctgg
     4081 tgagcggcga gcgggtcgag gcccacggcg agaccatctc ggcgaacctc gccctcgaga
     4141 tcccgggcgc ccagcccgtg ccggtgcagg tggccgccct cggcccccag atgctcaagc
     4201 tcgccggggc ccgcaccgcc gggacgatca cctggatgac cggtccccgc accatcgccg
     4261 accacaccgc ccccaccatc cgggccgcgg ccgaggacgc cggcaacccc gaccccttcg
     4321 tcaccagcgg cgtcccggtg tgcgtcaccg acgacgtcga gggcacccgg gcgcgtgccg
     4381 cgcaggtgta cgccatctac ggccagctcc cctcgtatcg ggcgatgctc gaccgcgagg
     4441 gcctggaggg tcccgccgac ctggcgatca tcggcgacga ggacaccgtc gccggccgga
     4501 tccgcgactt cgccgacgcc ggcgtcaccg acctggcggc cagcgagttc gggtccagca
     4561 gcgacgagcg gacccgcacc cgcgagctgc tccgcagcct gctgtgaagg tccgcgtcgg
     4621 ctacggcctc ggcacccgca cgatcaccaa cgacgccgag cgcttcgccg cgctggtcga
     4681 cgccctcgag tcgctccggt tcgactcgct ctggttgtcg gagaagatca ccgacgactg
     4741 ccccgacccg gtcgtcgccc tgtccttcgc cgcggcgcgc acggccaagc tgaagctcgg
     4801 caccagcgtg atggtcctac ccgggcgcaa cccgatgctg ctggccaagg agctggccag
     4861 cctcgaccgc ctctccgggg ggcggctcct gccggccttc gggctcggcc aggtcgaccc
     4921 cgccgagcag caggccttcg gcgtcgagcg caccgagcgg gcgaagcgct tcgacgaggc
     4981 cctccccctg ctccggcgct tctggaccga ggacgccgtc gaccacgacg gc
//
LOCUS       DATISU010000266         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_62240, whole genome shotgun sequence.
ACCESSION   DATISU010000266 DATISU010000000
VERSION     DATISU010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62240"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /gene="infA"
                     /locus_tag="VLR27_10545"
     CDS             complement(<1..213)
                     /gene="infA"
                     /locus_tag="VLR27_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HSP03932.1"
                     /translation="MAKNNDMIVMEGVVNESLPNASFRVELDNGHLVLAHISGKMRRH
                     RIRVLPGDRVQVELSPYDLTRGRLTFR"
     gene            340..1773
                     /gene="sucB"
                     /locus_tag="VLR27_10550"
     CDS             340..1773
                     /gene="sucB"
                     /locus_tag="VLR27_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HSP03933.1"
                     /translation="MADVEMPQLGETVTEGTITRWFKQVGDSVAEDEPLFEVSTDKVD
                     SEVPSPMSGTLSEILVEEGDTVDVGTVLARIGDAGDAPAEAPAEEEAPAAEPEEPQAE
                     APADDVDVEGEKEPSAQQPAQVPEGQASPEPEAPKKQEAPSGAGSGQGGGRSKLLSPV
                     VRKLVNDNDLDVDALEGTGPGGRITRDDVLDAIDAGAAKKGAAPAQPAAEAAPKAAAP
                     QQAAAPKPAAKAPAAPAPKAGERDTAVPHTRIRRATAEHMRRSVDTSAHVYASIEVDF
                     DAVDRVRAAHKEQFKADEGFSLTYLPFISRALVDAIREFPKVNASFGDDELIVHNYVN
                     LGIAVDLDFDALLVPVVSNADQLRMRAIGRTIMDLAGRARSKKLSPDELSGGTFTITN
                     PGPYGTTMTMPIINQPQVAILSTDAVKRRPVVIDLPDGSEAIGIHPTGNLTLTWDHRA
                     FDGAYAAAFLARMKELLETMDWAQELG"
     gene            complement(1819..>2364)
                     /locus_tag="VLR27_10555"
     CDS             complement(1819..>2364)
                     /locus_tag="VLR27_10555"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSP03934.1"
                     /translation="HSPYGVTKLAAEHLCGLYAANHGLPTVSLRYFTVYGPRQRPDMA
                     FHRLIEGALDQEPFPLYGDGSHVRDFTYVGDVVRANVLAGTADAEPGTVVNVCAGGSA
                     VLRDVIDAVGDAVGAPVPIQQHPEQPGDVQRTGGSNEAARRLLGWEPRTSLADGIAAQ
                     VAWHRHRRSGSRGVTPARPGG"
BASE COUNT          369 a          856 c          831 g          308 t
ORIGIN      
        1 gcggaaggtg agccggcccc gggtgaggtc gtaggggctg agctcgacct ggacgcgatc
       61 gcccggcagc acgcggatgc ggtgccggcg catcttgccc gagatgtggg cgagcaccag
      121 gtgcccgttg tccagctcca cgcggaacga cgcgttgggg agcgactcgt tcacgacgcc
      181 ctccatcacg atcatgtcgt tgttctttgc cacggatctc ctcgatcgtc gtcttcgggt
      241 tcgccccgga cgacgtgcac gagccgttcg gagcccctcc acgatggcac gatcacggcc
      301 cgaatcctcc attgccgggc cgccgataga gtccgagcca tggccgacgt cgagatgccg
      361 cagctcggag agaccgtcac cgaggggacg atcacccgct ggttcaagca ggtgggcgac
      421 tccgtcgccg aggacgagcc gctcttcgag gtgtccaccg acaaggtcga ctccgaggtg
      481 ccgtcgccga tgagcggcac gctcagcgag atcctggtgg aggagggcga cacggtcgac
      541 gtcggcacgg tcctggcccg catcggcgac gccggtgacg caccggccga ggcgccggcg
      601 gaggaggagg ccccggccgc cgagccggag gagccccagg ccgaagcgcc ggccgacgat
      661 gtggacgtcg agggcgagaa ggagccctcc gcccagcagc cggcccaggt ccccgagggc
      721 caggcctcac ccgagcccga ggcaccgaag aagcaggagg cgccctcggg tgccgggtcc
      781 ggtcagggcg gcggccgctc gaagctgctg tccccggtcg tgcgcaagct ggtgaacgac
      841 aacgacctcg atgtcgacgc cctcgagggc accggccccg gcggtcgcat cacccgcgac
      901 gacgtcctcg acgccatcga cgccggcgcg gccaagaagg gtgccgcacc tgcgcagccc
      961 gccgccgagg ccgctccgaa ggccgccgcg ccgcagcagg ctgccgctcc gaagccggcg
     1021 gccaaggctc cggctgcgcc cgcacccaag gccggcgagc gcgacaccgc cgtcccgcac
     1081 acccgcatcc gccgggccac cgccgagcac atgcgtcgct cggtcgacac ctcggcccac
     1141 gtgtacgcct ccatcgaggt cgacttcgac gccgtcgacc gggtgcgcgc cgcccacaag
     1201 gagcagttca aggccgacga gggcttcagc ctcacctacc tgcccttcat cagccgggca
     1261 ttggtcgacg ccatccgtga gttccccaag gtcaacgcca gcttcggtga cgacgagctc
     1321 atcgtccaca actacgtgaa cctcggcatc gcggtggacc tcgacttcga cgcgctgctc
     1381 gtgcccgtcg tgagcaacgc cgaccagctg cgcatgcggg ccatcggtcg cacgatcatg
     1441 gacctcgccg gccgggcccg ctccaagaag ctgagccccg acgagctgtc cggtggcacg
     1501 ttcaccatca cgaaccccgg gccgtacggc acgacgatga ccatgccgat catcaaccag
     1561 ccccaggtcg cgatcctgtc caccgacgcg gtgaagcgcc gcccggtcgt gatcgacctg
     1621 cccgacggct ccgaggccat cggcatccac cccaccggca acctcaccct cacgtgggac
     1681 caccgggcct tcgacggcgc ctacgccgcc gcattcctgg cccgcatgaa ggagctgctc
     1741 gagacgatgg actgggccca ggagctcggc tgaatccgtt ctggcagtca cggcgtcaca
     1801 cccggcagcg cagtggcctc agcccccggg gcgagcgggt gtgacgcctc gactgccaga
     1861 acgcctgtgg cggtgccagg cgacctgggc ggcgatgccg tcggccagtg aggtccgggg
     1921 ttcccagccg agcagccggc gggcggcctc gttgctgcca ccggtgcgct ggacgtcgcc
     1981 gggctgctcg gggtgctgct ggatcgggac cggtgcaccg accgcgtcgc ccacggcatc
     2041 gatgacgtcg cgcaggacgg ccgagccgcc ggcgcagacg ttgacgacgg tgcccggctc
     2101 cgcatcggcg gtgccggcca gcacgttggc ccgcacgacg tcgccgacgt aggtgaagtc
     2161 gcggacgtgg gagccgtcgc cgtagagggg gaacggctcc tggtcgaggg caccttcgat
     2221 gaggcggtgg aacgccatgt ccgggcgctg tcgggggccg tagacggtga agtagcgcag
     2281 cgacaccgtg gggaggccgt ggttcgcggc gtagaggccg cacaggtgct cggcggccag
     2341 cttggtcacg ccgtaggggc tgtg
//
LOCUS       DATISU010000267         1695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_388802, whole genome shotgun sequence.
ACCESSION   DATISU010000267 DATISU010000000
VERSION     DATISU010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388802"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..>1695
                     /locus_tag="VLR27_10560"
     CDS             184..>1695
                     /locus_tag="VLR27_10560"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HSP03935.1"
                     /translation="MTETVSGTGAKAGPAGRTLTIERVFSTEGVHPYDEITWERRDVV
                     QTNWKTGETVFEQRGVEYPDFWSVNASTIVTTKYFRGAVGTDAREWSLKQLIDRVVKT
                     YRKGGEDFGYFTTTEQAELFEHELTWLLVHQYFSFNSPVWFNVGTPSPQQVSACFILS
                     VDDSMDSILNWYKEEGFIFKGGSGAGLNLSRIRSSKELLSSGGTASGPVSFMRGADAS
                     AGTIKSGGATRRAAKMVVLDVDHPDIVEFVETKAREEDKIRALRDAGFDMDLGGKDIT
                     SVQYQNANNSVRVTDEFMRAVEDGTEFGLKARGTGEVIETVDARELFTKISKAAWECA
                     DPGLQYDDTINDWHTNPETGRITASNPCSEYMSLDNSSCNLASLNLMKFLKDDDTFDG
                     ELFAKAVDVIITAMDISICFADFPTESIGDTTRDYRQLGIGYANLGALLMAMGLGYDS
                     DGGRAMAASITSLMTGQSYKRSAELAGVVGPYNGYARNAEAHKRVMRKHQAAND"
BASE COUNT          310 a          607 c          537 g          241 t
ORIGIN      
        1 gctccggatg gagcgcgagc actcctcggt cgacccccct cagcccgctc cgacgggctg
       61 accccagacc gcccggcgtg tgtagtgggg aagctgcacg cgccgggcgc accaacgtcg
      121 gtggtcgcca ccaccatgta agagcaggac cgaacggcac gaccgaggac cagaggagat
      181 tccatgaccg agacggtgag cgggaccggc gcgaaggccg gcccggccgg gcggacgctg
      241 acgatcgagc gggtgttcag caccgagggc gtgcacccgt acgacgagat cacgtgggag
      301 cgccgtgacg tcgtgcagac caactggaag accggcgaga ccgtcttcga gcagcgtggc
      361 gtcgagtacc ccgacttctg gtcggtcaac gcgagcacga tcgtcaccac caagtacttc
      421 cgcggcgccg tcggcaccga cgcccgcgag tggagcctca agcagctgat cgaccgggtc
      481 gtgaagacct accgcaaggg cggcgaggac ttcggctact tcaccaccac cgagcaggcc
      541 gagctcttcg agcacgagct gacctggctc ctggtccacc agtacttctc gttcaactcc
      601 ccggtctggt tcaacgtcgg caccccctcg ccgcagcagg tcagcgcctg cttcatcctc
      661 tcggtcgacg actcgatgga ctccatcctc aactggtaca aggaggaggg cttcatcttc
      721 aagggcggct ccggtgccgg cctcaacctc tcccgcatcc ggtcctccaa ggagctcctc
      781 tcctccggcg gtacggcgtc cgggccggtc tccttcatgc gcggcgccga cgcctcggcc
      841 ggcaccatca agtccggtgg cgccacccgt cgcgcggcga agatggtcgt gctggacgtc
      901 gaccaccccg acatcgtcga gttcgtcgag accaaggcgc gcgaggagga caagatccgc
      961 gcgctgcgtg acgccgggtt cgacatggac ctcggcggca aggacatcac ctcggtccag
     1021 taccagaacg ccaacaactc ggtccgcgtc accgacgagt tcatgcgcgc cgtcgaggac
     1081 ggcaccgagt tcggcctgaa ggcccgcggc accggcgagg tcatcgagac cgtcgacgcc
     1141 cgcgagctgt tcaccaagat ctcgaaggcc gcgtgggagt gcgccgaccc gggcctgcag
     1201 tacgacgaca cgatcaacga ctggcacacc aacccggaga cgggccggat caccgcgtcc
     1261 aacccgtgct cggagtacat gtcgctcgac aactcctcgt gcaacctggc gtcgctgaac
     1321 ctgatgaagt tcctcaagga cgacgacacc ttcgacggcg agctgttcgc gaaggccgtc
     1381 gacgtgatca tcaccgcgat ggacatctcc atctgcttcg ccgacttccc gaccgagtcg
     1441 atcggcgaca ccacccgcga ctaccgccag ctcggcatcg gctacgccaa cctcggcgcg
     1501 ctgctgatgg cgatgggcct gggctacgac tccgacggcg gccgcgcgat ggcggcgtcg
     1561 atcacgtcgc tgatgaccgg ccagtcctac aagcgctcgg cggagctcgc cggcgtggtc
     1621 ggcccctaca acggctacgc ccgcaacgcg gaggcccaca agcgggtcat gcgcaagcac
     1681 caggcggcca acgac
//
LOCUS       DATISU010000268         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_388878, whole genome shotgun sequence.
ACCESSION   DATISU010000268 DATISU010000000
VERSION     DATISU010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388878"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..636)
                     /locus_tag="VLR27_10565"
     CDS             complement(115..636)
                     /locus_tag="VLR27_10565"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HSP03936.1"
                     /translation="MGKPFIVRVGDLLREPGSRRLLDVEVEAERLETSAASTIPGTPL
                     TLEGAVVAMAGGVEVIGTLGFDWYGACRRCLDDVTGHTDVPLREIAQRTPLDDEIYPI
                     DDDLLDLQPMVDELVLASLPIAPLCAEDCPGPDPERFPTTTEDEAAAAAAAEETPPDP
                     RWAALSELRFDEG"
     gene            complement(637..1272)
                     /locus_tag="VLR27_10570"
     CDS             complement(637..1272)
                     /locus_tag="VLR27_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03937.1"
                     /translation="MSDRPGQSPYEAPQVESVLRELRDLIEQARPMPLSTSVMINQAE
                     VLDLLNEATERLPEELRSAKWLLKQREEFLARTRRESEEIIEAARQKADQLVQQTEVV
                     KAAEARARSVVDAADAESRRLKLEAEDFCDQRLASFEIVLERTMKVVSAGRAKLQGSQ
                     AAQDERDLGDAPEGGHVQTPEDEQAAHERRARRRLEAVPDSGQGFFDQDDR"
     gene            complement(1269..1745)
                     /gene="coaD"
                     /locus_tag="VLR27_10575"
     CDS             complement(1269..1745)
                     /gene="coaD"
                     /locus_tag="VLR27_10575"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HSP03938.1"
                     /translation="MRTVLYPGTFDPIHNGHLEIIETASRLFDKVTVAAMRNPQKGEP
                     LFSFEDRKAMIEESTSHLDNVEVVQFATLVVDLAREVGADVIVKGLRAVSDFESEMHQ
                     AHMNRKISGVETLFIPSATEHSFVASKLLREIARFGGNVNEMVPDAVVKRLAEKFG"
     gene            complement(1742..>2251)
                     /gene="rsmD"
                     /locus_tag="VLR27_10580"
     CDS             complement(1742..>2251)
                     /gene="rsmD"
                     /locus_tag="VLR27_10580"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HSP03939.1"
                     /translation="PTTDRVREAMFNALGSLGAVDGAVVADLFAGSGALGIEALSRGA
                     ASAHFVESDRRAVAVIEENLATLGLDDRGVVLRRPVEGALDDLPAPLDLVLADPPYAF
                     EGWAALLGEVAPRLADDGLVVIESGRAIDLPPGWERVRERTYGGTVVLFARPEPPATG
                     APDPTGAHP"
BASE COUNT          300 a          769 c          845 g          337 t
ORIGIN      
        1 ttcttgggga cggccatggc tgttcaacct cagatgcggt gccgggaggg cgttcgaatg
       61 cgtccgggcc agtcggttgc cgaccaggcg gacgcgaagg agacacccta gcggtcagcc
      121 ttcgtcgaac ctcaactcgg agagggcggc ccagcgggga tccggcgggg tctcctcggc
      181 cgcggcggcc gcggccgcct cgtcctcggt cgtggtgggg aagcgctccg gatcggggcc
      241 ggggcagtcc tcagcgcaca ggggggcgat ggggaggctg gcgagcacca gctcgtcgac
      301 catgggctgc aggtcgagca ggtcgtcgtc gatggggtag atctcgtcgt cgaggggtgt
      361 ccgctgggcg atctcgcgca gcgggacgtc cgtgtggccg gtgacgtcgt cgaggcagcg
      421 gcggcacgcg ccgtaccagt cgaagccgag cgtgccgatg acctcgaccc cgccggccat
      481 ggccacgacc gcgccctcga gggtcagtgg ggtgccgggg atggtgctgg ccgccgaggt
      541 ctcgaggcgc tcggcctcga cctccacgtc gagcaggcgg cggctgcccg gctcgcgcag
      601 caggtcgccg acgcggacga tgaagggctt gcccacctag cggtcgtcct ggtcgaagaa
      661 cccctgaccc gagtcgggga ccgcctccag ccggcgtcgg gcccggcgct cgtgcgcggc
      721 ctgctcgtcc tcgggcgtct gcacgtgccc gccctcgggc gcgtcgccga ggtcgcgctc
      781 gtcctgggcg gcctggctgc cctggagctt ggcccggccg gccgagacga ccttcatggt
      841 gcgctcgagc acgatctcga agctggccag gcgctggtcg cagaagtcct cggcctcgag
      901 cttgaggcgg cgggactcgg catcggcggc atcgacgacg gagcgggcgc gggcctcggc
      961 ggccttgacc acctcggtct gctgcacgag ctggtcggcc ttctggcggg ccgcctcgat
     1021 gatctcctcg gattcgcgcc gggtccgggc caggaactcc tcgcgctgct tgagcagcca
     1081 cttggccgac cgcagctcct cgggcagccg ctcggtggcc tcgttcagca ggtcgaggac
     1141 ctcggcctgg ttgatcatca ccgaggtgga cagcggcatc ggccgcgcct gctcgatgag
     1201 gtcgcgcagc tcgcgtagca cggactccac ctggggggcc tcgtagggcg actggccggg
     1261 gcggtcgctc atccgaactt ctccgccagg cgcttcacca cggcgtcggg gaccatctcg
     1321 ttcacgttgc cgccgaagcg ggcgatctcg cgcagcagct tggaggcgac gaaggagtgc
     1381 tcggtggccg aggggatgaa cagggtctcc acgccggaga tcttgcggtt catgtgggcc
     1441 tggtgcatct cggactcgaa gtccgacacg gcccgcaggc ccttgacgat cacgtcggca
     1501 cccacctcgc gggccaggtc gacgacgagg gtggcgaact gcacgacctc gacgttgtcc
     1561 aggtgcgacg tgctctcctc gatcatcgcc ttgcggtcct cgaaggagaa cagcggctca
     1621 cccttctgcg ggttgcgcat ggcggccacg gtgaccttgt cgaacagccg tgaggcggtc
     1681 tcgatgatct ccaggtgacc gttgtggatc ggatcgaacg tcccgggata gaggaccgtc
     1741 ctcacggatg tgctcctgtc ggatcggggg cgccggtggc cggtggctcc ggccgggcga
     1801 acagcacaac cgtaccgccg taggtgcgct ccctcaccct ttcccagccg ggcgggaggt
     1861 cgatggcgcg gccggactcg atgacgacga ggccgtcgtc ggcgagccgc ggggcgacct
     1921 caccgagcag cgcggcccac ccctcgaacg cgtacggggg gtcggccagg accaggtcga
     1981 ggggcgccgg caggtcgtcg agggcgccct cgaccgggcg gcgcaggacc acgccgcgat
     2041 cgtcgaggcc cagcgtcgcg aggttctcct cgatcacggc gacagcccgc cggtcggact
     2101 cgacgaagtg cgcgctggcc gccccacggc tgagcgcctc gatgccgagc gcgccgctgc
     2161 cggcgaacag gtcggcgacc acggcgccat cgaccgcgcc gaggctcccg agggcgttga
     2221 acatggcctc gcgcacccgg tcggtggtgg g
//
LOCUS       DATISU010000269         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_248587, whole genome shotgun sequence.
ACCESSION   DATISU010000269 DATISU010000000
VERSION     DATISU010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248587"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VLR27_10585"
     CDS             <1..526
                     /locus_tag="VLR27_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HSP03940.1"
                     /translation="RVPVTEAQGRIHTSSATVTVLPEIDDVEIDIDEKNDLEIQVYRS
                     SGPGGQSVNTTDSAVRITHKPSGLVVSMQDEKSQLQNKAKALRVLKSRLFQLEEERQR
                     AELSSARRDQVGGGGRSEKIRTYNYKENRITDHRIGLTLYKLDKVLAGDLDDVSDALV
                     QDEQAERLADATAG"
     gene            complement(634..2178)
                     /locus_tag="VLR27_10590"
     CDS             complement(634..2178)
                     /locus_tag="VLR27_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03941.1"
                     /translation="MEDTELLEALDRRAGRAVAALDAGLAGAEPAPLPIEPGRGRRRP
                     WVPLLVAAALVVVALVGIGLALDGRDDEQGTIADDRDVPVETDDGVPGFALAEPDVLG
                     YEIRAAFDGPSVAVDPASAQPGPPYTAHGPTGSDDPWQQAIVTQTTPWDERVYGGELI
                     DLGGVEGLYRTSGSSQQIAWQDGDTAQTLMTNALDRDALVEVAREAVAGGWSGDGALP
                     GHDVLHGGTYDQFGPIIEYGANVRSDWRGVAYDDTSGDDDVVLGWRQGGPGLLGALHV
                     FGDEVERIDVDGQRVSISRSSVGAPSHASWEPVEGTVVTLVTYGDIDAVLGQLVPALA
                     PIDADAFAALLTSHPAAPEDPLLDVPGSQTDPGEGTVVASVELPDRAGYSYRVAIVEQ
                     APSAFALMQEVGDGNGTSGSGGPLGDLTTPQLQAGSYSEQEGGTSTPLIVSGVLPAGV
                     APDLSTARFVDRRTGAEVAVLDFLTGTIPGSPNLLGLVLLDADPLEVRVDVTFQTTDG
                     ERTWRL"
BASE COUNT          336 a          831 c          819 g          292 t
ORIGIN      
        1 gcgcgtgccg gtcaccgagg cccagggccg catccacacc tcgtcggcca cggtcaccgt
       61 gctgcccgag atcgacgacg tcgagatcga catcgacgag aagaacgacc tcgagatcca
      121 ggtgtaccgc tcgtccggcc cgggcgggca gtcggtcaac accaccgact ccgccgtgcg
      181 catcacccac aaaccgtcgg gcctggtggt gtcgatgcag gacgagaaga gccagctcca
      241 gaacaaggcc aaggccctgc gcgtgctgaa gagccggctc ttccagctgg aggaggagcg
      301 ccagcgggcc gagctctcgt cggctcggcg cgaccaggtg ggcggcggcg gtcgcagcga
      361 gaagatccgg acctacaact acaaggagaa ccggatcacc gaccaccgca tcggcctcac
      421 gctctacaag ctcgacaagg tcctcgccgg cgacctcgac gacgtgtccg acgccctcgt
      481 ccaggacgag caggccgagc gcctcgccga cgccaccgcc ggctgacccc cgcagccctg
      541 acggcggcgc ccccggcaaa ctggcagtcg tggtgtagcg ccgggttctc gggctgctgc
      601 gtcacgccgg tgtgacgccg tgcgcagaga gggtcagagc cgccaggtcc gctcgccgtc
      661 ggtggtctgg aacgtcacgt ccacgcggac ctcgagcggg tcggcgtcca gcaggaccag
      721 gccgagcagg ttgggcgaac ccgggatggt gccggtcagg aagtcgagca cggccacctc
      781 ggcacccgtg cggcggtcca cgaaccgggc agtggacaga tccggcgcga cgccggcggg
      841 cagcacgccg ctgacgatga gcggggtgct ggtgccgccc tcctgctccg agtagctgcc
      901 cgcctgcagc tgcggggtgg tgagatcccc cagcggtcca ccggacccgc tcgtgccgtt
      961 cccgtccccg acttcctgca tgagcgcgaa ggcgctgggt gcctgctcga cgatcgcgac
     1021 ccggtacgag tagccggccc ggtcggggag ctcgaccgac gcgacgaccg tcccctcgcc
     1081 gggatcggtc tgggatcccg ggacgtcgag gagcgggtcc tcgggggcag ccgggtggga
     1141 ggtcaagaga gccgcgaagg cgtcggcgtc gatcggcgcg agggcgggga cgagctgacc
     1201 gaggaccgcg tcgatgtccc cgtaggtgac gagggtgacg accgtgccct ccaccggttc
     1261 ccacgacgcg tgcgacgggg cacccacgct cgacctggag atcgagaccc gctggccgtc
     1321 cacgtcgatc cgctccactt cgtcgccgaa cacgtgcagg gcgccgagca ggccgggccc
     1381 gccctggcgc cagccgagca cgacgtcgtc gtccccggag gtgtcgtcgt aggcgacccc
     1441 gcgccagtcg ctgcgcacgt tggcgccgta ctcgatgatc gggccgaact ggtcgtaggt
     1501 gcctccgtgc agcacgtcgt gcccgggcag cgctccgtcg ccggaccatc cccctgccac
     1561 tgcctcacgg gcgacctcga cgagggcgtc ccggtccaag gcgttcgtca tgagggtctg
     1621 ggcagtgtcc ccgtcctgcc aggcgatctg ctgggagctc ccggaggtgc ggtacaggcc
     1681 ctcgacgcca ccgaggtcga tgagctcgcc gccgtagacc cgctcgtccc acggcgtggt
     1741 ctgcgtgacg atcgcctgct gccacggatc gtcgctgccg gtcggaccgt gcgcggtgta
     1801 gggcgggccc ggctgggcac tggcgggatc gaccgcgacg gagggtccat cgaacgcggc
     1861 ccggatctcg tagccgagga cgtcgggctc ggcgagcgcg aaccctggca cgccgtcgtc
     1921 ggtctcgacc ggcacgtcgc ggtcgtcggc gatcgtgccc tgctcgtcgt cccgaccgtc
     1981 cagcgccagc ccgatcccca ccagcgcgac cacgaccaag gccgcggcga ccaggagggg
     2041 cacccacggg cgccggcgac cgcgcccggg ctcgatcggc aggggagcgg gttcggcgcc
     2101 agccagcccg gcgtcgagcg cggcgacggc acgaccagcc cggcggtcca gcgcctcgag
     2161 cagctcggtg tcctccatca cgcctcctcc agctcgtcgc ggagcagctc ggcgagcgcc
     2221 agccggcccc ggtgcaggtg gaccttgacg gtgccctcgg cgatgtcgag cacccggg
//
LOCUS       DATISU010000270         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_295799, whole genome shotgun sequence.
ACCESSION   DATISU010000270 DATISU010000000
VERSION     DATISU010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295799"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VLR27_10595"
     CDS             <1..219
                     /locus_tag="VLR27_10595"
                     /inference="COORDINATES: protein motif:HMM:NF014595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03942.1"
                     /translation="HAHLADDANRISVSLDHSIWFHRPVDASGWLLSEMTPIASGHGR
                     GLAIGSVRTRDGHLVATIAQEALLRERS"
     gene            273..>2143
                     /locus_tag="VLR27_10600"
     CDS             273..>2143
                     /locus_tag="VLR27_10600"
                     /inference="COORDINATES: protein motif:HMM:NF016169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HSP03943.1"
                     /translation="MFTIHRSERADALVDALVEVVATPLPDPFAAEVVAVPTRGVERW
                     LTHRLGATVGATPGRSDGVCANVEFPFPGRLVGDVLAVATGVDRDEDPWRPERLVWPV
                     LSVLDRCAGEPWLRMVGAHLGVRGPGVEADEHDPLRRRRRHPAARHIAELFDRYGVFR
                     PSMVLAWLDRTSADDEADPDLPPDARWQPELFRRVRDHVGVASPPERIADAVERLRAD
                     RVDLDDVLPDRVSLFGLTRLPATYLEVLRALATHRDVHLFALHPSLALWDRIADAHAG
                     VVPLVAAGLPRAEDPTRREPRHPLLATWAHDSREMQLVLSAAGEAQVAHRAPAPPRWS
                     DSLLSRLQEAIRDDEPVALLPGTEGDRSVQVHACHGRGRQVEVLRDAICHLLAADPTL
                     EPRDVIVLCPDIDEYAPLLHATFGSRDPDDPEHLPGPPVLPYRLADRSLRQTNPLLGA
                     LADLLDLLDDRLTATEVLRFAGRPPVRRRFAFDDDALERIAGWVERSGVRWGLDAEHR
                     RPWSLQSVADHTWRAGLDRVLLGVAMDTDDARTVGGRLPLDDVDSGDIDLAGRLAELV
                     DRLDGVVAHCCEDRPVADWVALLGEATDLLLAAPPAEAWQRVQVDAVLRDVLAESSR"
BASE COUNT          245 a          886 c          738 g          274 t
ORIGIN      
        1 cacgcccacc tcgccgacga cgccaaccgc atctcggtgt cgctcgacca cagcatctgg
       61 ttccaccggc ccgtggacgc gtccggctgg ctgctctcgg agatgacccc catcgcgtcg
      121 ggccacggcc ggggcctggc catcgggtcg gtccgcaccc gggacgggca cctcgtcgcc
      181 accatcgccc aggaggccct gctccgagag cggtcctgag tccccacccg gtggtcggcg
      241 ctgtcacagc gatcgactac gaaggggcgg agatgttcac gatccaccgc tcggagcggg
      301 ccgacgccct cgtggacgcc ctcgtcgagg tcgtggccac gccgctgccc gacccgttcg
      361 cggccgaggt cgtggccgtg ccgacccgcg gcgtcgagcg ctggctcacc caccgcctgg
      421 gcgccacggt cggagccacc cccggccggt ccgacggcgt gtgcgccaac gtggagttcc
      481 ccttccccgg ccgcctggtc ggtgacgtgc tcgccgtcgc gacaggcgtc gaccgcgacg
      541 aggacccgtg gcggccggag cggctggtgt ggccggtgct cagcgtgctc gaccgctgcg
      601 ccggcgaacc ctggctgcgc atggtcggcg cccacctcgg ggtgcgcggt ccgggcgtcg
      661 aggccgacga gcacgacccc ctccgccgaa ggcgccggca cccggccgcc cgccacatcg
      721 ccgagctgtt cgaccgctac ggcgtgttcc gcccgtcgat ggtgctggcc tggctcgacc
      781 ggacgagcgc ggacgacgag gccgaccccg acctgccgcc cgatgcccgc tggcagcccg
      841 agctcttccg ccgggtgcgg gaccacgtcg gtgtggcatc cccgcccgag cgcatcgccg
      901 acgcggtcga gcgcctgcgg gccgaccggg tcgatctcga cgacgtcctg ccggaccggg
      961 tctcgctgtt cggcctgacc cgcctgcccg ccacgtacct cgaggtgctg cgggcactcg
     1021 ccacccaccg cgacgtgcac ctgttcgcgc tgcacccgtc gctggcgctg tgggaccgca
     1081 tcgccgacgc ccacgccggc gtcgtccccc tggtcgccgc cggcctcccc cgggccgagg
     1141 acccgacccg ccgggagccc cgccacccgc tgctcgccac ctgggcccac gacagccgcg
     1201 agatgcagct cgtgctctcc gccgccggcg aggcccaggt cgcccaccgg gccccggctc
     1261 caccccggtg gtcggactcg ctcctcagcc gactgcagga ggcgatccga gacgacgagc
     1321 ccgtggcact cctgccgggc accgagggag accggtccgt ccaggtccac gcctgccacg
     1381 gccggggccg ccaggtcgag gtgctgcgcg acgccatctg ccacctcctc gccgccgacc
     1441 ccaccctcga gccacgcgac gtcatcgtcc tgtgccccga catcgacgag tacgccccgc
     1501 tgctgcacgc cacgttcggc tcccgggacc ccgacgaccc cgagcacctg ccggggccgc
     1561 ccgtgctccc ctaccgcctg gccgaccgct cgctgcgcca gaccaacccg ctgctgggcg
     1621 ccctcgccga cctgctcgac ctgctcgacg accgcctcac cgccaccgag gtcctgcgct
     1681 tcgccggtcg tccgccggtg cgccgtcggt tcgccttcga cgacgacgcc ctcgagcgca
     1741 tcgccggctg ggtcgagcgc agcggcgtgc gctggggcct cgacgccgag caccgtcgcc
     1801 cgtggtcgct gcagtcggtg gccgaccaca cctggcgggc cggcctcgac cgggtgctgc
     1861 tcggcgtggc catggacacc gacgacgccc gcaccgtcgg cggacggctg ccgctcgacg
     1921 acgtggacag cggcgacatc gacctggccg gccgcctggc cgagctggtc gaccgactcg
     1981 acggagtggt ggcgcactgc tgcgaggacc gaccggtggc cgactgggtc gcgctgctcg
     2041 gcgaggccac cgacctgctg ctggccgcac cgccggccga ggcgtggcag cgggtgcagg
     2101 tggacgccgt gctccgcgac gtgctggccg agtcgtcccg cga
//
LOCUS       DATISU010000271         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_668022, whole genome shotgun sequence.
ACCESSION   DATISU010000271 DATISU010000000
VERSION     DATISU010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668022"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VLR27_10605"
     CDS             <1..835
                     /locus_tag="VLR27_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HSP03944.1"
                     /translation="DEHKWEPSGRFGAVAGVDGRIGTAAMPGTFAQVGMEYLHDNDYS
                     GEAFELFARISVKNHKHSTLNPLAAYTKEMSLEQVMGDVMIAYPNTRPMCSANCDGGA
                     AAVVVSGAKLKTLSKEQQRRAVKVSASVLTSDPWQEACQVLPDVNTLTRMAAKQAYEQ
                     AGVSPEDLDLVELHDCFATAELVHYDNLMLCEPGGAVDFFESGATWRDGSTPVNVSGG
                     LESKGHPISATGRANIWEVCHHLRGEAGDRQIEGAKVGMAHVIGLGSACGVHILESSA
                     A"
     gene            complement(875..2122)
                     /locus_tag="VLR27_10610"
     CDS             complement(875..2122)
                     /locus_tag="VLR27_10610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSP03945.1"
                     /translation="MPGTPNSNHHDVAIVGAGLAGLVAARTLIAAGRDVVVLEAADAP
                     GGRVRTDLVDGHRLDRGFQVLLTDYPELPRHLDLDALDLRRFDPGALVRVGDRFHRVV
                     DPFRSPVAGLRGALAPVGSIGDKLRVGLLRRRLLRATAPELLRGADVTTAQALRDDGF
                     SPAMIERFFEPLVGGIQLDPSLGTSRRVFDVVFRSLARGDSAVPALGMEEIPRQLAAG
                     LPAGALRCDSAVTRVEDGRVTLGDGEAVTATHVVVATEGPAAARLLGTEPVGSRSASC
                     VWFGAVSAPVDDRLVILDGTGDGPARNVAVMSNVAPTYAPDGRALVAAAVPGRADSSL
                     EPAVRAQLRGWWGPQVDGWVHLRTDAIAHGQPDQSPPFAPKQAVAVRPGLWVCGDHRD
                     TGSIQGAMFSGRRCAEAVLAAPA"
     gene            2190..>2549
                     /locus_tag="VLR27_10615"
     CDS             2190..>2549
                     /locus_tag="VLR27_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HSP03946.1"
                     /translation="MESTRIDRWLWAVRIYKTRSDATKACSGGHVELDGRTVKPAQKV
                     KVGDRVHAQVGQRQRVLDVARIIDKRVGAPIAVECYVDHSPPPPERPLQALFGERERG
                     AGRPTKRERRQLDRELGR"
BASE COUNT          372 a          932 c          954 g          291 t
ORIGIN      
        1 cgacgagcac aagtgggagc cctcgggccg cttcggtgcc gtcgccggcg tcgacggccg
       61 catcggcacc gcggccatgc cgggcacctt cgcccaggtc ggcatggagt acctgcacga
      121 caacgactac agcggcgagg cgttcgagct gttcgcccgc atcagcgtga agaaccacaa
      181 gcactccacg ctgaacccgc tggccgccta caccaaggag atgagcctcg agcaggtgat
      241 gggcgacgtg atgatcgcct accccaacac ccgcccgatg tgctcggcca actgcgacgg
      301 cggcgccgcc gcggtggtcg tctccggcgc caagctgaag acgctctcca aggagcagca
      361 gcgccgggcc gtcaaggtct cggcgtcggt gctgacgtcg gatccttggc aggaggcctg
      421 ccaggtgctg cccgacgtga acaccctcac ccgcatggcg gccaagcagg cctacgagca
      481 ggccggcgtg tcccccgagg acctcgacct ggtcgagctg cacgactgct tcgccaccgc
      541 cgagctggtg cactacgaca acctgatgct gtgcgagccc ggtggggcgg tggacttctt
      601 cgagtccggc gccacctggc gcgacggttc gaccccggtc aacgtctccg gcggcctcga
      661 gtccaagggg cacccgatct cggccaccgg ccgcgccaac atctgggagg tctgccacca
      721 cctccggggc gaggccggcg accgccagat cgagggggcc aaggtgggca tggcccacgt
      781 catcggcctc ggctcggcct gcggcgtcca catcctggag tccagcgcgg cctgatccgc
      841 accgctgcct gacgtccgcc cgaggcggtg ggcgtcaggc aggggcagcg agcacggcct
      901 cggcgcagcg gcgcccggag aacatcgcgc cctggatgga gccggtgtcg cggtggtccc
      961 cgcacaccca cagccccggt cgcaccgcca ccgcctgctt cggcgcgaac gggggcgact
     1021 ggtcgggttg gccgtgggcg atcgcgtcgg tgcgcaggtg cacccacccg tccacctgcg
     1081 gcccccacca gccgcgcagc tgggcgcgca ccgctggctc cagcgagctg tcggcacgcc
     1141 ccggcacggc ggcggcgacc agggcccgcc cgtccggcgc gtaggtcggc gcgacgttgc
     1201 tcatcacggc cacgttgcgg gccggtccgt cgccggtgcc gtcgaggatc accagccggt
     1261 cgtccaccgg tgccgagacc gcgccgaacc acacgcacga cgccgatcgg gacccgaccg
     1321 gctccgtgcc gagcaggcga gcggccgccg gaccctcggt ggccaccacg acgtgggtgg
     1381 cggtcacggc ctcaccgtcg ccgagggtga cccgtccgtc ctcgacgcgg gtgaccgccg
     1441 agtcgcagcg gagcgcgccg gcgggcaggc cggccgccag ctggcggggg atctcctcca
     1501 taccgagggc gggcacggcc gaatcgccgc gggcgaggct gcggaacacc acgtcgaaga
     1561 cccgacggct ggtgccgagc gacgggtcga gctggatgcc gcccacgagg ggttcgaaga
     1621 agcgctcgat catcgccggc gagaagccgt cgtcccgcag cgcctgcgcc gtcgtcacgt
     1681 ccgcgccccg cagcagctcc ggggcggtgg cccgcagcag ccgccggcgc agcaggccga
     1741 cgcgcagctt gtcgccgatc gagcccaccg gcgcgagcgc gccgcggagc ccggcgacgg
     1801 gggagcggaa cggatcgacg acccggtgga agcggtcgcc gacccgcacg agggcaccgg
     1861 ggtcgaaccg gcgcagatcg agggcgtcga ggtccaggtg gcgggggagc tcggggtagt
     1921 cggtgagcag cacctggaag ccgcgatcga gccggtgccc gtcgaccagg tcggtgcgga
     1981 cccgtccgcc cggtgcgtcg gcggcctcga gcacgacgac gtcccggccg gcggcgatga
     2041 gggtgcgggc ggcgaccagg ccggcgagcc cggcacccac gatggcgacg tcgtggtggt
     2101 tcgagttcgg ggtgccgggc acggagcgac ggtagcgacg gcgctcgtcc cggcgaccgc
     2161 ggctcggcct gctgcgcgac catgggtggg tggagagcac tcgcatcgac cggtggctgt
     2221 gggccgtgcg gatctacaag acccggtccg atgcgacgaa ggcctgcagc ggcggccatg
     2281 tcgagctcga cgggcgcacc gtgaagccgg cccagaaggt gaaggtgggc gaccgcgtcc
     2341 acgctcaggt cggacagcgc cagcgggtcc tcgacgtggc ccgcatcatc gacaagcggg
     2401 tgggcgcgcc catcgcggtc gagtgctacg tggaccacag cccgccgccc ccggaacgac
     2461 cgctgcaggc cctcttcggc gagcgcgagc gcggcgccgg acggcccacg aagcgggagc
     2521 gccgccagct cgaccgggag ctcggccgg
//
LOCUS       DATISU010000272         1582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_202807, whole genome shotgun sequence.
ACCESSION   DATISU010000272 DATISU010000000
VERSION     DATISU010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202807"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VLR27_10620"
     CDS             <1..268
                     /locus_tag="VLR27_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HSP03947.1"
                     /translation="REIFSVPRIGKIAGCMVQSGVITRNSKVRFLREGTIIWKGSIQS
                     LRRFKDDATEVRAGFECGVGLSDFQDLKEGDIIETFEEREIERV"
     gene            265..561
                     /locus_tag="VLR27_10625"
     CDS             265..561
                     /locus_tag="VLR27_10625"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HSP03948.1"
                     /translation="MIWSGAALELTLRIPLGDSLKAKRSVIRPILDGAHRRFGVAASE
                     VADQDVHDRAVLVFAAVASSPGHVDEVLDAVDRFVWSFPEVEVVDSGRYWVDTA"
     gene            672..938
                     /locus_tag="VLR27_10630"
     CDS             672..938
                     /locus_tag="VLR27_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HSP03949.1"
                     /translation="MATDQLPGGVVHELPDDLREGLEASAVALEAWHDITPLARNEFI
                     CWVEDAKRPPTRERRIRRTREELEEGQRRPCCWPGCKHRERTGR"
     gene            complement(973..1143)
                     /locus_tag="VLR27_10635"
     CDS             complement(973..1143)
                     /locus_tag="VLR27_10635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HSP03950.1"
                     /translation="MADVSILDLRNHSDKIVERAERGERLTITRAGTPVAELTALPRP
                     TVSLEVVSVPQT"
     gene            complement(1182..>1582)
                     /locus_tag="VLR27_10640"
     CDS             complement(1182..>1582)
                     /locus_tag="VLR27_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HSP03951.1"
                     /translation="PIGAGRTSREIPRRLRRMLERRDRCCRVPGCGATAGLHAHHLIH
                     WEDEGPTELWNLALVCPFHHRLHHRGLITIRGPADDLTVIDRRDRLLSGGSLARPPTG
                     PFPDAPCYRHVDGERAQWKWYDPPVVRTPN"
BASE COUNT          224 a          533 c          585 g          240 t
ORIGIN      
        1 ccgcgagatc ttctccgtgc cccgcatcgg caagatcgcc ggttgcatgg tccagagcgg
       61 cgtcatcacg cgcaactcca aggtccgctt cctgcgtgag ggcaccatca tctggaaggg
      121 ctcgatccag tcgctgcggc ggttcaagga cgacgccacc gaggtgcgcg ccggcttcga
      181 gtgcggtgtc ggtctgtcgg acttccagga cctcaaggaa ggcgacatca tcgagacctt
      241 cgaggagcgc gagatcgagc gggtgtgatc tggagtggcg cagctctcga gctgaccctc
      301 cgcatcccgc tcggcgattc tctgaaggcc aagcggtcgg tgattagacc gatcctcgac
      361 ggtgcgcacc ggcgcttcgg cgtcgccgct tccgaggtcg cagaccagga cgtccacgac
      421 cgggcggtcc tggtcttcgc cgcggtcgcc tccagcccgg gtcacgtgga cgaggtcctc
      481 gacgccgtcg accgcttcgt gtggtcgttc cccgaggtcg aggtggtcga cagcggccgc
      541 tactgggtcg acacggcctg agcgcgacgt cgggctgacg cacccgttgg gtccgaagtg
      601 ccacgtcgga gcgacaatcc gacggcacga atgtcgcagc tggctgcgcc acccatcggt
      661 agcgtgggcc cgtggccacc gatcagctgc cgggcggggt cgtgcacgag cttcccgacg
      721 acctgcgcga gggcctggag gccagcgccg tcgcgctcga ggcctggcac gacatcacgc
      781 cgctggctcg caacgagttc atctgctggg tggaggacgc caagcgccca cccacccgcg
      841 agcgccggat ccggcgcacc cgcgaggagc tggaggaggg gcagcgccgg ccgtgctgct
      901 ggcccggctg caagcaccgg gagcgcaccg gccgctgaga gcgaccgcag aggcattgct
      961 cccttgggtg cttcaggtct gcgggacgga gaccacctcg agggagaccg tcggacgggg
     1021 cagggcggtg agctcggcca ccggggtgcc ggcgcgagtg atcgtcagcc gctcgccccg
     1081 ttcggcgcgc tcgacgatct tgtcgctgtg gttccgcagg tcgaggatcg acacgtctgc
     1141 cacgggcggc accgtatgtg gcctgattcg cacggcggcc atcagttggg cgtccgcacg
     1201 accggcgggt cgtaccactt ccactgcgct cgctcgccgt ccacgtgccg gtagcacggg
     1261 gcgtcgggga acggtcccgt gggtggtcgg gcgaggctgc cgccggacag caaccggtcc
     1321 cgcctgtcga tcaccgtgag gtcgtcggcc gggccccgga tggtgatgag gccacggtgg
     1381 tgcaggcgat ggtggaaggg gcagacgagg gcgaggttcc acagctcggt cggaccctcg
     1441 tcctcccagt ggatgaggtg gtgggcgtgg agcccggcgg tggcaccgca ccccggcacc
     1501 cggcagcagc ggtcccgccg ctcgagcatc cggcgcagtc ggcgggggat ctcccggctg
     1561 gtgcgtccgg cgccgatcgg ca
//
LOCUS       DATISU010000273        12102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_295967, whole genome shotgun sequence.
ACCESSION   DATISU010000273 DATISU010000000
VERSION     DATISU010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12102
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295967"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="VLR27_10645"
     CDS             complement(<1..1626)
                     /locus_tag="VLR27_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSP03952.1"
                     /translation="MSGDARVLVIDDDPDVSLLARLQLENAGYDVVEAATAADGLSRA
                     LDTAPDVVLLDWMLPDGDGIGLLAELRASDATSDVPVIMVTARSHAGDQRAAWEAGVV
                     DYLTKPFDGRELLRCVRDAVADPAPARQEARRRDALERLSDRRVDPDRLSAVVEHASD
                     AIVTMSLDGAVLSWNRAAEELYGYRVDEVLGRPVAVLSPSGHRDEVADALERVAAGVA
                     VPHFETVRRGKEGTDISVSVTVSPIRDEQGHITSASAIARDITERKAEESLHRGLLEA
                     APDAMVVVDDTGTIHLVNAQTEALFGYERSELVGQAVEILVPEEYRSGHPGHRRGYFT
                     EPRTRPMGQDLELTARRKDGSVFPVEISLSSIETERGRLVSAAIRNVSERKAAEARFR
                     GLVDAAPDAMVIVDPQGRIELVNAQADTMFGYERGELVGRPVEVLVPPRFRDQHPGHR
                     STDAHQARVRPLGTGLELFGLRKDGSEFPVEISLSPLQTGDSVSISAAIRDVSVRKRA
                     EAVQTAAFEREREASRRLREVDRLRSDFLSTVSH"
     gene            complement(1762..1920)
                     /locus_tag="VLR27_10650"
     CDS             complement(1762..1920)
                     /locus_tag="VLR27_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03953.1"
                     /translation="MPDNNDPSKLEEPTPDEEKAAERAAEQSPDIEEDYRESTERGAN
                     QEGEGKIV"
     gene            2049..2825
                     /locus_tag="VLR27_10655"
     CDS             2049..2825
                     /locus_tag="VLR27_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HSP03954.1"
                     /translation="MNDAASPEPRPGSRDSAEDEAAGSLLGPDPDEEISGRERLAAVL
                     ERRLDPVMAVLAVAWGVFVAYDLIAPASQRPVLSLVSDVVWGIFLLEFVAKLIVSGHP
                     LRFLRRRWPSVFFLAVPLLRTARIIPALRTLRILPSVRVLGSSYRAIGTARGLLGGRL
                     SFLAVTTVSVIVAGAQLLYLLDGSGESFGETLWWSVNLAISGTYTFEPSTVPSRIVSL
                     LLSVYAVIVFASMAAALGAFFVDQRAEHAAAEEERSETDG"
     gene            2882..4084
                     /locus_tag="VLR27_10660"
     CDS             2882..4084
                     /locus_tag="VLR27_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03955.1"
                     /translation="MPSTSHPAAAIAAAASIAAGLIHAAAAGSHAELATLSMLFSITA
                     VAQIGWGAAVLVRPTPPVMVAGLGINLAAFGAWLTSRTVGLSAIEGLEAGQTIGFQDG
                     LAAGFALLALAAGTVAVADVSVPRLGQAAVPAIVALLAFATVPAMAQPHDHDSHDHDG
                     DVALAAGEVASAGDGHDDDHVHDESHDHGDADHHDDDHHGTADDDADETEPDAPTSEE
                     LGYPASFASFLDDAPDADARDRAEQLLIETTEAMQAFPDEAAVQAAGFTSIGDGATGY
                     EHYINVQRIIDPRVLDPDDIESIVLKVNPDGTKEVASAMYLLPFGATMADAPDIAGEL
                     TPWHDHQNLCWDGARVVGTTDATGTCARGEFRPTQPMLHVWVDEHPCGPFAGIEGSHG
                     SGCDQHDH"
     gene            4130..5278
                     /locus_tag="VLR27_10665"
     CDS             4130..5278
                     /locus_tag="VLR27_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP03956.1"
                     /translation="MSTVSSPFYDDDHHAYREVVRSFTEKEIAPHVHEWDLAGEVPRD
                     LYVKAGNIGLFGDGFQEEYGGHGQRDALMRLVLMEELCMAGSGGVVAALFSNYIGLPP
                     IQRFGSEEMKARVIAPCMSGEKIAALAITEPSGGSDVARIKTTARREGDEFVVNGSKT
                     FITSGMRADFFATAVRTGGEGPAGISMMLIEGDRPGVDRTKLDKMGWLASDTATLYFD
                     DVRVPEENLITEENAGFSAIVNNFNAERIDLAVQSTAFSRVCFDEAVGWARERETFGK
                     RLADHQVIRHKLAEMDRMINASKAWLELLSWRLNQGDNPVAEIAEAKVNATLCFEQCA
                     REAAQILGGASYLRGDTVERLYREVRVQAIGGGSEEIMRDLASRQLGI"
     gene            complement(5306..6697)
                     /locus_tag="VLR27_10670"
     CDS             complement(5306..6697)
                     /locus_tag="VLR27_10670"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HSP03957.1"
                     /translation="MARPREQQFERHMSDNEALMWNLEKDPALSSWFASLTILDRPPS
                     LDRLRARLARAVVAVPRLRQHVVASVGRLSPPVWVDDPEFDLDRHVWRIAVPAPGSDA
                     DLHELAMRLLQEPFDRTRPLWQFVVIEGLADGRAALFQKMHHTITDGEGGVRLAEQFV
                     DLERDPNEPDPPAVPAPAGDGDGFAESAMEVMGHNVRRVGGLARRVTGSVVGNPLGTV
                     QGLGRAVKEIDRSLRPSHEEGRTVGSPLWKPRTLKRWFGSLHVPLADVKQAGRVLGGT
                     INDVFVAGALAGAAAYHHERGVHPDLLRAAMPVSTRKGGTSGGNAFAITTTDLRVPDD
                     PIEAFAVVHEALATVKGGGSMDLVGQLAMVVNLLPTSVLTSFAKDAAATVDFTVSNVR
                     AAPVELYIAGGRCEAVLPMGPISGTAFNLSTLSYNGSLDMGLVVDAGAIDDPAGLRDH
                     LVTAFDTLIEVGR"
     gene            6732..8705
                     /locus_tag="VLR27_10675"
     CDS             6732..8705
                     /locus_tag="VLR27_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HSP03958.1"
                     /translation="MSVRGSQRRNHVRAWWRDARAGVVADAVDRLGEADGQAFGVRVD
                     VVVDELHEAVDDVYGALGHDPDDLVLRLLSLALSTAEARSAELRILDHRREIDPHWFL
                     RARQVGYVAYTDRFAGTLRGVIDRLDHLDELGITYLHLMPLLRPRDGENDGGYAVADY
                     GAVDPRLGTMADLEALTGALHDRDMALCIDLVVNHTAAEHPWAQAALAGDPTHRRYYR
                     IFPDRTEPDEYEATMREVFPEMAPGSFTEVEGLGWVWTTFREWQWDLDYENPDVLASM
                     CAVMGDLANRGVDVFRLDAVPFLWKQKGTDCENLPQAHRIVQILRNVLRISAPGTICK
                     AEAIVSPDQLVQYLGGHEPEVRECELAYHNQLMVMLWSSLAARDAQLMTRSLERMRRP
                     PDGTGWVTYVRCHDDIGWAVSDEDAAAVGWSGFEHRAFLNRYYSGEHPGSWAQGVKFQ
                     ENPVTLDARISGTTAALSGIDQARDRHDPVALDSAIRRFLLLHSVIIGWGGSVPVLYM
                     GDEIALANDRSFADDPDHADDNRWIHRPPMDWEAAARRHDPKSVEGRVHRGLRQQIQA
                     RRDLPVMRAGGTTSAMWTGHRSVFAWRRTHPRHLPFLGLAAFADEPTSVPLPLAVEAG
                     LHDPVPVLASDGDVHIDDGQIHLPAWGWVWLSD"
     gene            complement(8710..10257)
                     /locus_tag="VLR27_10680"
     CDS             complement(8710..10257)
                     /locus_tag="VLR27_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HSP03959.1"
                     /translation="MIRRIPLAPVAVASLLVIAVTWWIDDRGDPDVGPVAASEVDDLD
                     TEPAETTTTLPPFEGWSNPALAGEPYPGATVEGLLTFRGNPTRSFHGRGPIPTDPTID
                     YRYPAEGGLCGESTNLGETKVWCGMGWTGQPAVFEREAADGSVRTWLVFGAYDGAVHF
                     LDANTGERILPAFPTGDLIKGSVTVDPDGYPLVYTGSRDNEYRILSIEGDEAVELWSM
                     HADDFSPILWNDDWDSSGIVIDDHLFVGGENSRFVIVKLNRGTAPDGSVTVAPEIVFD
                     VAGWDQELLDAIGDQTVSIEVSPTVVGDTVYFTNSGGLVQGYDISGLADGAEPTRSFR
                     YWMGDDTDASIVADADGMLYVAAEYERAATVERSDAVGQLVKLDPTADVAAGEDPLVW
                     SWFDNEVRPGGVWGTPAVVDGVVYVGTNGGRLVAVSATTGVELWSKRLPGPLWSSPTV
                     IDGVLLMGDCTGDFHAFGLGEGGEEPEELWSVSLGGCIEATPAVWEGRIYIGSRDGAL
                     YALAGGA"
     gene            10291..10734
                     /locus_tag="VLR27_10685"
     CDS             10291..10734
                     /locus_tag="VLR27_10685"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HSP03960.1"
                     /translation="MRADRFEPLPDDVLERWSTFGRWPDRQYFPGLLGLEIEELKSDY
                     CRMRLPFRPEHEQPAGVVHGGALATLMDTVVVPAIGSAYGPETQYMTVTMNLSYIGAV
                     KEADVVAEGWVTRRGRSTVFCRAEAMCDDRLVMEGSLVYAVREPR"
     gene            10744..11982
                     /locus_tag="VLR27_10690"
     CDS             10744..11982
                     /locus_tag="VLR27_10690"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSP03961.1"
                     /translation="MPETLRYVSRVARLPLQDGDGETIGQVQDVVLAPAGRLAPRVLG
                     FITVVQRRRIFVNANRIGDLEATGVRMRTGSIDLRPFRKREGELLALGDLVDSPVEVD
                     GVEQYVRDVGISPSDSPGSMWELSTIALGGRGPLARRRTPVIVPWEACRSLFDVGADA
                     AEVAEMREMRPADVANRIRALPLEKRQRLAEIMEDERLADLLEELPEDEQIRLINGLD
                     LERAADILEEMDPDDAADLLGEMSAEERARLLEAMEPDEAHHVRRLLTYESATAGGLM
                     TPEPVIMSPESTVAEALAMLREASLPVALAAQVFVVNPPLSVPTGRFLGTIGFQRLLR
                     YPPSENLGDIVPDRSEHLSPDTPQLEVAGRLAAYDMVAVAVCDGARHLVGVVTIDDVM
                     DHLLPTGWRRLLPRHRPGVG"
BASE COUNT         1669 a         4480 c         4252 g         1701 t
ORIGIN      
        1 gtgggagacg gtggacagga agtcggatcg gagccggtcc acctcccgca ggcgtcggga
       61 tgcctcccgc tcccgctcga aggccgcggt ctgcaccgcc tcggcccgct tgcgcacgct
      121 gacgtcgcgg atggcagcag agatcgagac gctgtcgccg gtctgcagcg ggctgaggga
      181 gatctccacg gggaactcgc tgccgtcctt gcgcagaccg aacagctcga ggccggtgcc
      241 cagcggccgc acccgggcct ggtgggcgtc ggtgctgcgg tggcccgggt gctggtcgcg
      301 gaagcggggc ggcacgagga cctcgacggg ccggccgacc agctccccgc gctcgtaccc
      361 gaacatggtg tcggcctgcg cgttcaccag ctcgatccgg ccctgcgggt cgacgatcac
      421 catggcgtcg ggggcggcgt cgacgaggcc acggaaccgg gcctctgccg ccttgcgctc
      481 ggacacgttg cggatcgccg ccgacaccag gcgcccgcgc tcggtctcga tcgacgacag
      541 cgagatctcc accgggaaca ccgagccgtc cttgcggcgg gcggtcagct cgaggtcctg
      601 gcccatcggg cgggtgcggg gctcggtgaa gtagccgcgg cggtgcccgg ggtggccgct
      661 gcggtactcc tcggggacga ggatctccac cgcctggccg accagctcgc tgcgctcgta
      721 gccgaacagc gcctcggtct gggcgttgac gaggtggatc gtgccggtgt cgtcgaccac
      781 gaccatggcg tcgggcgcgg cctcgagcag cccgcggtgg agcgactcct cggccttgcg
      841 ctcggtgatg tcccgggcga tggcggaggc cgacgtgatg tgaccctgct cgtcgcggat
      901 cggcgacacc gtcaccgaca ccgagatgtc cgtgccctcc ttgccacgcc gcacggtctc
      961 gaagtgcggc acggccacgc cggcggccac ccgctcgagg gcgtcagcta cctcgtcacg
     1021 gtggccggac ggcgacagca ccgccaccgg acggccgagg acctcgtcga cccggtaccc
     1081 gtacagctcc tcggcggccc ggttccagct caggaccgcc ccgtccaggg acatggtgac
     1141 gatggcgtcg gaggcatgct ccaccaccgc cgagagccgg tcggggtcga cccgacgatc
     1201 gctcagccgc tccagcgcgt cgcgccgccg ggcctcctgt cgggccgggg ccgggtccgc
     1261 caccgcatcg cgcacgcagc gcagcagctc acgaccgtcg aagggcttgg tcaggtagtc
     1321 gacgacgccg gcctcccacg ccgcccgctg gtcgccggcg tggctgcggg ccgtcaccat
     1381 gatcaccggc acgtcggagg tggcatcgct ggcccgcagc tcggcgagca ggccgatgcc
     1441 gtccccgtcg ggcagcatcc agtcgagcag gaccacgtcg ggcgcggtgt cgagggcgcg
     1501 ggacagcccg tccgccgccg tggccgcctc caccacgtcg tagccggcgt tctccagctg
     1561 caggcgcgcc agcagggaga cgtccggatc gtcgtcgatc accagcaccc gtgcgtcccc
     1621 cgacaccgat ccgttcctcc cagctccggg cgcagctcgc cgagtctgcc cctccggcgc
     1681 aggtccgggc gcaccggagc ccggacgcgg gacgagcacc cgtccgagga caggtgctcg
     1741 tcgagcagcg cagggtctcg atcagacgat cttgccctcg ccctcctggt tcgcaccgcg
     1801 ctcggtggac tcgcggtagt cctcctcgat gtcgggagac tgctcggcgg cccgctcggc
     1861 cgccttctcc tcgtcggggg tgggctcttc gagcttgctg gggtcgttgt tgtccggcat
     1921 accggcctcc tgcatgtggg tggggagcga gggcctggcc cccgcgcggt cggtgccgtc
     1981 cctacccagg cgcgctccga tgtgccccgg ctcgggtacc cctgcgccga tcgggttagg
     2041 gagctggcat gaacgatgcc gcttcccccg agccgcgccc cggctcccgg gacagcgccg
     2101 aagacgaggc ggcagggtcc ctcctcgggc ctgatcccga cgaggagatc agcgggcggg
     2161 agcggctggc ggccgtgctc gagcggcgcc tcgaccccgt gatggccgtg ctggccgtcg
     2221 cctggggcgt gttcgtggcg tacgacctca tcgccccggc cagccagcgt ccggtcctgt
     2281 ccctcgtcag cgacgtcgtc tgggggatct tcctcctcga gttcgtggcc aagctcatcg
     2341 tgtccggcca cccgctccgg ttcctgcgcc gacggtggcc gtcggtgttc ttcctggccg
     2401 tcccgctcct gcgcaccgcc cgcatcatcc cggcgctgcg caccctccgg atcctgccca
     2461 gcgtccgggt cctgggctcg tcgtaccggg ccatcggcac cgcgcgcggg ctgctcgggg
     2521 gccggctgtc gttcctcgcc gtcaccaccg tctcggtgat cgtcgccggc gcccagctgc
     2581 tctacctgct cgacggcagc ggggagagct tcggcgagac gctgtggtgg tcggtgaacc
     2641 tggcgatctc gggcacctac acgttcgaac ccagcaccgt cccgtcgcgg atcgtgtcgc
     2701 tgctgctcag cgtctacgcc gtcatcgtct tcgcctccat ggccgcggcc ctcggtgcgt
     2761 tcttcgtcga ccagcgcgcc gagcacgccg ccgccgagga ggaacggagc gagaccgacg
     2821 gctgagcccc gccgttcggc acctacgctg gagacgtccg tcacaccaga gaggggcacg
     2881 catgccctcg acatcccacc ccgctgcggc catcgctgcg gcggcatcca tcgccgccgg
     2941 cctgatccac gccgcggcgg ccggctccca cgccgagctc gccaccctgt cgatgctgtt
     3001 ctcgatcacc gcggtcgccc agatcggctg gggtgccgcc gtgctcgtcc gaccgacccc
     3061 gccggtcatg gtcgccgggc tcggcatcaa cctcgccgcc ttcggcgcct ggctcacgag
     3121 ccgcacggtc gggctctccg cgatcgaggg cctcgaggcc ggccagacca tcggcttcca
     3181 ggacggcctc gccgccggct tcgccctcct cgcgctcgcc gccgggacgg tcgccgtggc
     3241 cgacgtctca gtgccgcgcc tcggccaggc cgcggtgccg gccatcgtcg cgctgctcgc
     3301 cttcgccacg gtgcccgcca tggcccagcc ccacgaccac gacagccacg accacgacgg
     3361 cgacgtggcg ctggccgccg gcgaggtcgc ctccgccggc gacgggcacg acgacgacca
     3421 cgtccacgac gagtcccacg accacgggga cgccgaccac cacgacgacg accaccacgg
     3481 cacggccgac gacgacgcgg acgagaccga gccggacgcc cccacctcgg aggagctcgg
     3541 ctacccggcc agcttcgcgt cgttcctcga cgacgccccc gatgccgacg cgcgggaccg
     3601 ggccgagcag ctcctcatcg agaccaccga ggccatgcag gcgttcccgg acgaggccgc
     3661 ggtccaagcc gccggcttca cctccatcgg cgacggcgcc acgggctacg agcactacat
     3721 caacgtgcag cgcatcatcg acccccgggt gctcgacccc gacgacatcg agtcgatcgt
     3781 gctgaaggtc aaccccgacg gcaccaagga ggtggcctcg gccatgtacc tgctgccgtt
     3841 cggcgccacg atggccgacg cgcccgacat cgccggtgag ctcacgccct ggcacgacca
     3901 ccagaacctg tgctgggacg gggcccgggt ggtcggcacc accgacgcca ccggcacctg
     3961 cgcccgcggc gagttccggc cgacccagcc catgctccac gtctgggtgg acgagcaccc
     4021 ctgcggtccc ttcgccggca tcgagggctc gcacgggtcg ggctgcgacc agcacgacca
     4081 ctgatcccac ctgcccccgc ctgagcggcg tgtggaaggc tcggggacga tgagcacggt
     4141 gtcgagcccc ttctacgacg acgaccacca cgcctaccgg gaggtggtgc gctccttcac
     4201 cgagaaggag atcgcccccc acgtccacga gtgggacctg gccggcgagg tgccccgcga
     4261 cctgtacgtc aaggcgggga acatcgggct gttcggcgac gggttccagg aggagtacgg
     4321 cggccacggc cagcgcgacg ccctcatgcg cctggtgctc atggaggagc tgtgcatggc
     4381 cggatcgggc ggcgtggtcg ccgccctgtt ctccaactac atcggcctgc cgcccatcca
     4441 gcgcttcggc tccgaggaga tgaaggcccg ggtcatcgcc ccgtgcatga gcggcgagaa
     4501 gatcgcggcc ctggccatca ccgagccctc cggcggctcc gacgtggccc ggatcaagac
     4561 caccgcccgg cgcgagggcg acgagttcgt ggtgaacggc tcaaagacct tcatcacctc
     4621 gggcatgcgg gccgacttct tcgccaccgc ggtgcgcacc ggcggcgagg ggccggccgg
     4681 catctcgatg atgctgatcg agggcgaccg ccccggcgtc gaccgcacca agctcgacaa
     4741 gatgggctgg ctggcctcgg acaccgccac cctctacttc gacgacgtgc gggtgccgga
     4801 ggagaacctc atcaccgagg agaacgccgg cttctccgcc atcgtcaaca acttcaacgc
     4861 cgagcgcatc gacctcgccg tgcagtccac cgccttcagc cgggtctgct tcgacgaggc
     4921 cgtcggctgg gcccgcgagc gggagacctt cggcaagcgc ctcgccgatc accaggtgat
     4981 ccgccacaag ctggccgaga tggaccggat gatcaacgcg tccaaggcgt ggctggagct
     5041 gctgtcttgg aggctcaacc agggtgacaa cccggtggcc gagatcgccg aggccaaggt
     5101 caacgccacc ctgtgcttcg agcagtgcgc ccgggaggcg gcccagatcc tcggcggcgc
     5161 cagctacctg cggggcgaca ccgtcgagcg cctctaccgc gaggttcggg tccaggccat
     5221 cggcggcggc tccgaggaga tcatgcgcga cctcgcgtcg cgccagctcg gcatctgacc
     5281 gcgacaccag cactgccagt tctcctcagc ggccgacctc gatgagggtg tcgaaggcgg
     5341 tgacgaggtg gtcgcggaga ccggccgggt cgtcgatggc gccggcgtcc accaccaggc
     5401 ccatgtcgag gctgccgttg tagctgagcg tgctgaggtt gaacgccgtg ccggagatcg
     5461 ggcccatggg gaggaccgcc tcgcaccggc cgccggcgat gtagagctcg accggcgccg
     5521 cccgcacgtt ggagacggtg aagtcgacgg tggcggcagc gtccttggcg aacgacgtca
     5581 gcaccgacgt cggtagcagg ttgacgacca tggccagctg gccgacgagg tccatcgacc
     5641 caccgccctt caccgtggcc agcgcctcgt ggaccacggc gaaggcctcg atggggtcgt
     5701 cgggcacgcg caggtcggtg gtggtgatgg cgaacgcgtt gccgccggag gtgccgccct
     5761 tgcgggtgct caccggcatc gcggcgcgca ggaggtcggg gtggacgccg cgctcgtggt
     5821 ggtaggcggc ggcgccggcg agggcgccgg cgacgaagac gtcgttgatg gtcccgccga
     5881 ggacgcggcc ggcctgcttc acgtcggcga gcggcacgtg cagcgagccg aaccagcgct
     5941 tgagcgtgcg gggcttccac agcggcgacc cgacggtgcg gccctcctcg tgcgacgggc
     6001 gcagcgagcg atcgatctcc ttcacggccc ggccgaggcc ctggacggtg ccgagcgggt
     6061 tgccgaccac cgagccggtg acccggcggg cgaggccgcc gacccggcgc acgttgtggc
     6121 ccatgacctc catggcgctc tcggcgaagc cgtcgccgtc gccggccggg gcgggcacgg
     6181 cgggcgggtc gggttcgttg gggtcgcgct cgaggtcgac gaactgctcg gcgaggcgga
     6241 ccccgccctc accatcggtg atggtgtggt gcatcttctg gaacagcgcg gcccggccgt
     6301 cggccaggcc ctcgatgacc acgaactgcc acagcggccg ggtgcggtcg aagggctcct
     6361 gcagcagtcg catcgccagc tcgtgcaggt cggcgtcgct gccgggcgcg ggcacggcga
     6421 tgcgccagac gtggcgatcg aggtcgaact cggggtcgtc gacccacacg ggcgggctga
     6481 gtcggccgac gctggcgacg acgtgctggc gcaggcgcgg cacggccacg acggcacggg
     6541 cgaggcgggc ccgcaggcga tccagcgacg gcggccggtc gaggatcgtg aggctggcga
     6601 accaggagct gagcgcaggg tccttctcga ggttccacat gagcgcctcg ttgtcgctca
     6661 tgtggcgctc gaactgctgc tcccgggggc gggccatcct ggcgagcgta cccctgtacc
     6721 ttgcctcctc ggtgagcgtc cgaggcagcc agcgacggaa ccacgtccgc gcgtggtggc
     6781 gagacgctcg tgcgggcgtg gtggccgacg ccgtcgaccg cctcggcgag gcagacggcc
     6841 aggcgttcgg ggtccgggtc gacgtcgtgg tcgacgagct gcacgaagcc gtcgacgacg
     6901 tgtacggcgc actgggccac gaccccgacg acctcgtcct gcggttgctc tcactggcgc
     6961 tgtccacggc cgaggcacgg tctgcggagc tgcgcatcct ggatcaccgc cgggagatcg
     7021 acccgcactg gttcctccgg gcccgccagg tcggctacgt cgcgtacacc gaccggttcg
     7081 ccgggacgct gcgcggcgtc atcgaccggc tcgaccacct cgacgagctc ggcatcacct
     7141 acctgcacct catgccgctg ctgcgcccgc gcgacggcga gaacgacggc ggctacgccg
     7201 tcgccgacta cggcgccgtc gacccgcggc tgggcacgat ggcggacctc gaggccctca
     7261 ccggcgcgct gcacgaccgg gacatggcgc tgtgcatcga cctggtcgtc aaccacacgg
     7321 cggccgagca cccgtgggcc caggctgcgc tggccggtga ccccacccac cgtcgctact
     7381 accggatctt ccccgaccgc acggagcccg acgagtacga ggcgaccatg cgggaggtgt
     7441 tcccggagat ggcgccgggc agcttcaccg aggtcgaggg cctcggctgg gtgtggacga
     7501 cgttccgcga gtggcagtgg gacctcgatt acgagaaccc cgacgtgctg gcgtcgatgt
     7561 gcgcggtgat gggcgacctc gcgaaccggg gcgtcgacgt gttccgcctc gacgcggtgc
     7621 ccttcctctg gaagcagaag ggcaccgact gcgagaacct cccccaggcc caccggatcg
     7681 tgcagatcct ccgcaacgtg ctgcggatct cggcgccggg cacgatctgc aaggccgagg
     7741 cgatcgtgtc gcccgaccag ctggtgcagt acctgggggg ccacgagccc gaggtgcgcg
     7801 agtgcgagct cgcctaccac aaccagctca tggtgatgct gtggtcctcg ctcgccgccc
     7861 gggacgccca gctgatgacg agatcgctcg agcgcatgcg gcggccgccg gacgggacgg
     7921 gctgggtgac ctacgtgcgc tgccacgacg acatcgggtg ggcggtgagc gacgaggacg
     7981 cggctgccgt cgggtggagc ggcttcgagc accgcgcctt cctcaaccgc tactactccg
     8041 gcgagcaccc cggcagctgg gcgcagggcg tcaagttcca ggagaacccg gtgacgctcg
     8101 acgcccgcat ctccggtacc accgccgccc tcagcggcat cgaccaggcg cgggatcgcc
     8161 acgacccggt ggccctggac tcggcgatcc gccggttcct cctcctccac tcggtgatca
     8221 tcggctgggg cggctcggta ccggtgctct acatgggcga cgagatcgcc ctggccaacg
     8281 atcggtcgtt cgccgacgac cccgaccacg ccgacgacaa ccgctggatc caccgtccgc
     8341 cgatggactg ggaggcggca gcgcgccgac acgacccgaa gtccgtcgag gggagggtcc
     8401 accgcgggct gcgccagcag atccaggcgc ggcgcgacct gccggtcatg cgtgcgggcg
     8461 gcaccacttc ggcgatgtgg accgggcacc gctcggtgtt cgcgtggcgg cgcacgcacc
     8521 cccggcacct gcccttcctc gggctggcgg ccttcgccga cgagccgacg agcgtgccgc
     8581 tgcccctcgc cgtcgaggcc ggtctccacg acccggtccc cgtgctcgca tcggacggcg
     8641 acgtccacat cgacgacggg cagatccacc taccggcgtg gggatgggtt tggctgtcgg
     8701 actgatccgt caggcgccgc ccgccagcgc gtagagggcg ccgtcgcggc tgccgatgta
     8761 gatccggccc tcccacacgg cgggcgtcgc ctcgatgcag ccaccgaggg agaccgacca
     8821 cagctcctcg ggttcctcgc cgccctcccc caggccgaag gcgtggaagt caccggtgca
     8881 gtcacccatg agcagcacgc cgtcgatcac cgtcggcgac gaccacagcg gtccggggag
     8941 ccgcttggac cacagctcca cgccggtggt cgccgagacc gccaccaacc gtccaccgtt
     9001 cgtgccgacg tagaccacgc cgtcgacgac cgcgggcgtg ccccagaccc cgccgggccg
     9061 cacctcgttg tcgaaccacg accagaccag cggatcctcg ccggccgcca cgtcggccgt
     9121 ggggtccagc ttcaccagct ggccgacggc gtcggagcgc tcgacggtcg ccgcacgctc
     9181 gtactcggcg gcgacgtaga gcatcccgtc ggcgtcggcc acgatcgagg cgtcggtgtc
     9241 gtcgcccatc cagtagcgga aggaccgggt cggttcggcc ccgtcggcca ggccgctgat
     9301 gtcgtagccc tggacgaggc caccggagtt cgtgaagtac acggtgtcgc ccacgaccgt
     9361 gggcgagacc tcgatggaca ccgtctggtc gccgatcgcg tccagcagct cctggtccca
     9421 cccggccacg tcgaacacga tctcgggggc caccgtgacg gagccgtcgg gagccgtgcc
     9481 ccggttgagc ttcacgatga cgaagcggct gttctccccg ccgacgaaga ggtggtcgtc
     9541 gatgacgatg ccggagctgt cccagtcgtc gttccacagg atcggcgaga agtcgtcggc
     9601 gtgcatcgac cacagctcga cggcctcgtc gccctcgatg gagaggatcc ggtactcgtt
     9661 gtctcgggag ccggtgtaga cgagcgggta gccgtcgggg tcgaccgtga ccgagccctt
     9721 gatgaggtca ccggtgggga acgccgggag gatgcgctcg cccgtgttcg cgtcgaggaa
     9781 gtggacggcg ccgtcgtagg ccccgaagac gagccaggtg cggaccgagc cgtcggcggc
     9841 ctcccgctcg aagacggccg gctggccggt ccagcccatc ccgcaccaga ccttcgtctc
     9901 gccgaggttg gtggactccc cgcagagccc gccctcggcg gggtaccggt agtcgatcgt
     9961 cgggtccgtc gggatgggtc cgcggccgtg gaaggaccgg gtcgggttgc cccggaaggt
    10021 cagcaggccc tccaccgtcg cgcccgggta gggctcgccg gcgagcgccg ggttcgacca
    10081 gccctcgaac ggcggcagcg tcgtggtcgt ctctgccggc tccgtgtcca ggtcgtccac
    10141 ctccgacgcg gccacgggcc cgacatcggg gtcgccccgg tcgtcgatcc accacgtcac
    10201 cgcgatgacc aggaggctgg cgaccgccac cggtgccagc gggattcgac ggatcaccgg
    10261 cggagtgtac gaccgcgtag gttcccgatg gtgcgcgccg accgcttcga acccctcccc
    10321 gacgacgtcc tcgaacggtg gtccaccttc ggccgttggc cggaccgcca gtacttcccc
    10381 ggcctgctcg gcctggagat cgaggagctg aagtccgact actgccgcat gcggctcccg
    10441 ttccgccccg agcacgagca gccggccggc gtcgtgcacg gcggtgcgct cgccaccctc
    10501 atggacaccg tggtcgtgcc cgccatcggg tcggcgtacg gacccgagac ccagtacatg
    10561 accgtcacca tgaacctgag ctacatcggt gcggtgaagg aggcggacgt ggtcgccgag
    10621 ggctgggtca cccgccgggg tcgctccacc gtgttctgcc gggccgaggc gatgtgcgac
    10681 gaccgcctcg tcatggaggg atccctcgtc tacgcggtgc gcgagccccg gtagcctccg
    10741 cacgtgccgg agacgctgcg atacgtgtca cgcgtcgctc gcctccccct gcaggacgga
    10801 gacggcgaga cgatcgggca ggtgcaagac gtcgtcctcg cgcctgccgg ccgcctcgcg
    10861 ccccgggtgc tcggcttcat cacggtcgtg cagcgccgcc gcatcttcgt gaacgccaac
    10921 cgcatcggcg acctcgaagc cacgggcgtc cggatgcgca ccggctccat cgacctgcgt
    10981 cccttccgca agcgcgaggg cgagctgctc gccctcggcg acctggtcga cagcccggtc
    11041 gaggtcgacg gcgtcgagca gtacgtgcgc gacgtcggca tcagcccgtc ggactccccg
    11101 ggctcgatgt gggagctgtc caccatcgcg ctcggcggtc gcgggccgct ggcccgtcgc
    11161 cgcaccccgg tcatcgtgcc gtgggaggcg tgccggtcgc tgttcgacgt cggcgccgac
    11221 gcggccgagg tcgccgagat gcgcgagatg cgcccggccg acgtggccaa ccgcatccgg
    11281 gccctgccgc tggagaagcg ccagcgactg gccgagatca tggaggacga gcgcctcgcc
    11341 gacctgctcg aggagctgcc cgaagacgag cagatccggc tgatcaacgg gctcgacctg
    11401 gagcgggccg ccgacatcct cgaggagatg gaccccgacg acgccgccga cctcctcggg
    11461 gagatgagcg ccgaggagcg cgcccggctg ctcgaggcca tggagcccga cgaggcccac
    11521 cacgtgcgcc ggctcctcac gtacgagtcc gcgaccgccg gcggcctgat gaccccggag
    11581 ccggtgatca tgtcgccgga gtccacggtc gccgaggccc tcgccatgct gcgggaggcc
    11641 tcgctgcccg tcgccctcgc cgcccaggtc ttcgtcgtga acccgccgct gtcggtcccc
    11701 accggtcggt tcctcggcac catcgggttc cagcgactgc tccgctaccc gccgtcggag
    11761 aacctcggcg acatcgtccc cgatcgctcc gagcacctga gccccgacac cccccagctc
    11821 gaggtggccg ggcggctggc cgcctacgac atggtcgcgg tcgcggtgtg cgacggggcc
    11881 cggcacctgg tcggcgtggt caccatcgac gacgtgatgg accacctgct gcccaccggc
    11941 tggcggcgcc tgctccctcg tcaccgcccg ggggtcgggt agtgctgcgc ttccgccgct
    12001 ccgacgacct ggcccgcccc gcgcaggccg gcgtcatcgg cgtgcacttc gaccccgatg
    12061 cgttcggtcg gttctccgag ggcatcgccc gcttcctcgg ga
//
LOCUS       DATISU010000274         3614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_156261, whole genome shotgun sequence.
ACCESSION   DATISU010000274 DATISU010000000
VERSION     DATISU010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156261"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VLR27_10695"
     CDS             <1..882
                     /locus_tag="VLR27_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HSP03962.1"
                     /translation="LVPNSSTYGISKRLPDDERKRLRKILDGVKPDQHGVIVRTAAEN
                     VTAEEIENDVKRLVTLWDKIDDLAKRSQAPTLLYREPDMAVRVIREEFSKDYRAVVID
                     DPELYKEVDEYVSGITPALADRVQFYDPEAEKLPLFERHHIHEQLHRALDRKVWLPSG
                     GSLIIEHTEALTVIDVNTGKNVGKNSLEDTVYANNLEAAEEIARQLRLRDIGGIIVID
                     FIDMEVKKNRDDVGRVLREALARDKTRTQVFDISELGLVEMTRKRIGEGLLVGFTEEC
                     ETCKGRGVVFDKDLLDG"
     gene            930..1238
                     /gene="rplU"
                     /locus_tag="VLR27_10700"
     CDS             930..1238
                     /gene="rplU"
                     /locus_tag="VLR27_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HSP03963.1"
                     /translation="MYAVIATGGKQHRVAEGDVLEVERLGVEEGGSVDLRPVLLVDGD
                     TVLSTPDDLGGVSVTARVVGDAKGPKINGFVYKNKSNNRKRWGHRQHYTQIEITGISR
                     "
     gene            1260..1514
                     /gene="rpmA"
                     /locus_tag="VLR27_10705"
     CDS             1260..1514
                     /gene="rpmA"
                     /locus_tag="VLR27_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HSP03964.1"
                     /translation="MSKTKGGGSTRNGRDSESKRLGVKAYDGTVVSAGSILVRQRGTR
                     FHPGLNVGKGGDDTLFATSDGTVKFGSRHGRKLVDILPLD"
     gene            1607..2281
                     /locus_tag="VLR27_10710"
     CDS             1607..2281
                     /locus_tag="VLR27_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP03965.1"
                     /translation="MSGMEGPGGPSDVTVVVLEDHEMVASALEAVLDQDPRLRVVGTT
                     CRFEDALRVVAERSPTVLVTDLRLDGVEIASQLREFTEVCPDVRILVVTGWATERTLF
                     DSLDHGAHGFIGKAQGLDELVDAVVRVADGETVVAPQLLPALVRRTSARGGRDRTNLT
                     PRELDVLRLLAEGTGTREMATHLAVSPNTIRNHVRAVLGKLGAGSRLEAVTIARRMDL
                     IAPVDQ"
     gene            complement(2296..3459)
                     /locus_tag="VLR27_10715"
     CDS             complement(2296..3459)
                     /locus_tag="VLR27_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSP03966.1"
                     /translation="MADRPDHDPADGGPTPDLTPRAREWAATMAGHGSGSVRHAHADT
                     HADVGNTDIADREVREAREDDQLAPGATRAAGAGSRAVEEDPDPWRTCFERDRDRIVH
                     GTAFRRLAGKTQVFVFPDDHQRTRLTHALEVAQVATSIARACRLNVALAEAIALGHDC
                     GHGPFGHASEDALAPFVEGGYDHATWGADVVLAPLNLCTETLDGIRNHSWSRPAPSTP
                     EGEVVSWADRIAYLCHDFEDAVLAGVVGPDDLPDIVAERCGRDRRQQLHAFISAVVHA
                     TAESGTVGMHDDLAEALTAFRAFNYERIYLRDESRRQAVVVDRLLTALVEWYRADPAR
                     LVAEGRPIDDSTDIVREAVSFVGGMTDRFAHERALANLGWDAADLPIGLETHT"
     gene            3505..>3614
                     /locus_tag="VLR27_10720"
     CDS             3505..>3614
                     /locus_tag="VLR27_10720"
                     /inference="COORDINATES: protein motif:HMM:NF013205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HSP03967.1"
                     /translation="MSQFVDECGLHVKGGDGGAGSVSFRREAHVPRGGPDG"
BASE COUNT          546 a         1277 c         1287 g          504 t
ORIGIN      
        1 ctggtcccca actccagcac ctacggcatc tccaagcggc tgcccgacga cgagcgcaag
       61 cgcctccgca agatcctgga cggcgtcaag ccggaccagc acggcgtgat cgtgcgcacg
      121 gcggccgaga acgtgacggc cgaggagatc gagaacgacg tcaagcgcct ggtcaccctg
      181 tgggacaaga tcgacgacct ggccaagcgc tcgcaggcgc cgacgctgct ctaccgcgag
      241 cccgacatgg ccgtgcgggt catccgggag gagttctcca aggactaccg cgccgtggtc
      301 atcgacgacc cggagctgta caaggaggtc gacgagtacg tctcgggcat cacccctgcc
      361 ctcgccgacc gcgtgcagtt ctacgacccc gaggccgaga agctcccgct gttcgagcgc
      421 caccacatcc acgagcagct ccaccgggcc ctcgaccgca aggtgtggct cccctcgggc
      481 ggttcgctca tcatcgagca caccgaggcg ctcacggtca tcgacgtcaa caccggcaag
      541 aacgtgggca agaactccct cgaggacacc gtctacgcca acaacctcga ggccgccgag
      601 gagatcgccc gccagctccg cctgcgcgac atcggtggga tcatcgtcat cgacttcatc
      661 gacatggagg tgaagaagaa ccgcgacgac gtgggccggg tgctgcgcga ggcgctggcc
      721 cgggacaaga cccgcaccca ggtcttcgac atctccgagc tcggcctggt cgagatgacc
      781 cgcaagcgca tcggcgaggg cctgctcgtc ggcttcaccg aggagtgcga gacctgcaag
      841 ggtcgaggcg tcgtcttcga caaggacctc ctcgacggct gaggccgggt tggaggcggg
      901 acctgccacg ccgtacgatg tcgaacccta tgtacgcagt gatcgccacc ggcggaaagc
      961 agcatcgtgt cgccgaaggc gacgtcctcg aggtcgagcg cctcggggtc gaagagggtg
     1021 gctcggtcga cctgcggccc gtcctcctcg tcgacggtga caccgtcctg tccacccccg
     1081 acgacctggg tggcgtctcc gtcaccgctc gtgtggtggg tgacgccaag ggccccaaga
     1141 tcaacggctt cgtctacaag aacaagtcga acaaccggaa gcgctggggt caccgccagc
     1201 actacacgca gatcgagatc accggcatca gccgctgatc ccagaagagg tagctcgcca
     1261 tgtccaagac caagggcggc ggttccaccc ggaacggccg tgactccgaa tccaagcgcc
     1321 tcggcgtcaa ggcctacgac ggcaccgtcg tctcggccgg ctccatcctc gtgcgccagc
     1381 gcggcacccg cttccacccc ggcctgaacg tgggcaaggg cggcgacgac acgctgttcg
     1441 ccacgagcga cggcacggtg aagttcggtt cgcgccacgg gcgcaagctg gtcgacatcc
     1501 tcccgctcga ctgagcaccc cctcgaacgc actggcaccg gcccgggtcc tcgacccggg
     1561 ccgtgtcgcg cccgggggtg gtccatctgc actacgttct cgctcgatgt cagggatgga
     1621 ggggccgggc gggcccagcg acgtgaccgt ggtggtcctc gaggaccacg agatggtggc
     1681 cagcgccctc gaggccgtgc tcgaccagga tccccgcctg cgggtggtcg gcacgacgtg
     1741 ccggttcgag gacgccctgc gcgtggtcgc cgagcggtcg ccgaccgtgc tcgtcaccga
     1801 tctccgcctc gacggcgtcg agatcgccag ccagctccgc gagttcaccg aggtctgccc
     1861 cgacgtgcgg atcctcgtgg tcaccggttg ggccaccgag cgcaccctct tcgactcgct
     1921 cgaccacggc gcccacggct tcatcggcaa ggcccagggc ctcgacgagc tcgtcgacgc
     1981 tgtcgtgcgg gtcgccgacg gcgagacggt cgtggccccc cagctgctcc cggcgctggt
     2041 ccgccggacc tcggcacggg gcggacggga ccgcaccaac ctgacaccgc gagagctcga
     2101 tgtcctgcgg ctcctcgccg agggcaccgg cacccgcgag atggccacgc acctggcggt
     2161 gagccccaac acgatccgca accacgtgcg agcggtgctc ggcaagctcg gcgccggctc
     2221 ccgcctggag gccgtcacca tcgcccgccg catggacctg atcgccccgg tcgaccagta
     2281 ggggccgcac cggcctcagg tgtgggtctc gagcccgatc ggcaggtcgg cggcgtccca
     2341 gcccaggttg gccagggccc gttcgtgggc gaagcggtcg gtcatcccgc cgacgaagga
     2401 gacggcctcg cgcacgatgt cggtgctgtc gtcgatcggg cgcccttcgg ccaccaggcg
     2461 ggcgggatcg gcccggtacc actccaccag ggcggtgagg aggcggtcga cgacgacggc
     2521 ctgccggcgg gactcgtcgc gcaggtagat ccgctcgtag ttgaacgccc ggaaggcggt
     2581 gagcgcctcg gcgaggtcgt cgtgcatgcc caccgtgccc gattcggcgg tggcgtgcac
     2641 cacggcgctg atgaaggcgt ggagctgctg gcggcgatcc cgcccgcagc gctcggccac
     2701 gatgtcgggc aggtcgtcgg ggcccacgac gccggccagc acggcgtcct cgaagtcgtg
     2761 gcagaggtag gcgatgcggt cggcccagct gaccacctcg ccctcggggg tggacggcgc
     2821 cggccgcgac caggagtggt tgcggatccc gtcgagggtc tcggtgcaca ggttcagcgg
     2881 ggccaggacc acatcggcgc cccaggtggc gtggtcgtag ccaccctcga cgaagggggc
     2941 gagcgcgtcc tcggacgcgt gaccgaaggg tccgtggccg cagtcgtggc cgagggcgat
     3001 ggcctcggcg agggcgacgt tgagccggca ggcccgggcg atggacgtgg ccacctgggc
     3061 gacctcgagg gcgtgggtga ggcgggtgcg ctggtggtcg tcggggaaca cgaagacctg
     3121 ggtcttgccc gccaggcgcc ggaaggcggt gccgtgcacg atgcggtcgc ggtcgcgctc
     3181 gaagcaggtg cgccaggggt cggggtcctc ctcgaccgcc cgggatccgg cacccgcggc
     3241 ccgggtggcg cccggggcca gctggtcgtc ctcacgggcc tcgcggacct cgcggtcggc
     3301 gatgtcggtg ttgccgacat cggcgtgggt gtcggcgtgc gcgtggcgca ccgaaccgct
     3361 cccgtggccg gccatggtgg ccgcccactc ccgggcccgg ggggtcagat ccggcgtcgg
     3421 accgccgtct gcggggtcgt ggtcgggtcg gtcagccacg atccgagtct cccacgcgcc
     3481 ctggtcggtc ctagattccc cgcggtgtcc cagttcgtcg acgagtgcgg cctccatgtg
     3541 aagggcggag acggcggcgc cggctcggtg tcgttccgtc gcgaggccca cgtgccccgc
     3601 ggtgggcccg acgg
//
LOCUS       DATISU010000275         1908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_714794, whole genome shotgun sequence.
ACCESSION   DATISU010000275 DATISU010000000
VERSION     DATISU010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714794"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1244
                     /locus_tag="VLR27_10725"
     CDS             213..1244
                     /locus_tag="VLR27_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSP03968.1"
                     /translation="MQSDSHAHPAAELQAVTQAVKRVIVGQDRVLERLLVGLLAKGHL
                     LLEGVPGLAKTLAMETLATVVGGSFARIQFTPDLLPADITGTRVWRQSSERFDVELGP
                     VFVNFLLADEINRAPAKVQSALLEVMAEKQVSLGGETHQVPSPFLVMATQNPIESEGV
                     YPLPEAQRDRFLMKILIEYPSPAEESEIVLRHGIDPPEAERALDTGALLRLQDAADAV
                     SVDHGTLQYAVDLVQATRDPARYGLPDLVEYLSYGASPRATLGLVAAGRALALLRQRS
                     YVVPQDVFDVAPDVLRHRLVLSYESLAQGVGVEHVLARLLSTVPAPHVAGSPSASPPA
                     GDASEWAAG"
     gene            1241..>1908
                     /locus_tag="VLR27_10730"
     CDS             1241..>1908
                     /locus_tag="VLR27_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSP03969.1"
                     /translation="MSTTVHTEAAEVLRRLELDVNRRLDGLLHGDYRGLVPGHGSETG
                     ETRPYVPGDDVRRIDWNVSARSMDTHVRESIADRELETWVLVDRSPSLDFGTARTTKA
                     GLAVEAVGAIGFLTARTGNRLGAVIVDPDGTTVIPAKPGREHLLAVLHGLLTGARPGT
                     RLGGTSRHAGGTTDLGAALGHLIVPPRRRGLIVVVSDFLSTDGWSRALRGLTSRHDVV
                     AIEIV"
BASE COUNT          238 a          776 c          638 g          256 t
ORIGIN      
        1 ggtcgccgtc gagcaccggg gtgccctcgg tgccggggcc ggggacctgg cggccggctc
       61 gggccggggg ctcgatgccg ggagcgtcgg tggccctccg gttgaccagg acgacggcga
      121 cacctccgac caggacgacg acggcggcga tgagcaggag gatctccatg ggcgagcgag
      181 cctatcgtcg acccgcctct agccttcgag gggtgcagag cgactcccac gcccatcccg
      241 ccgccgagct ccaggctgtg acccaggcgg tcaagcgggt catcgtcggt caggaccgcg
      301 tgctcgagcg cctgctggtg ggcctgctgg ccaagggcca cctgctgctc gagggcgtgc
      361 cgggcctggc caagaccctc gccatggaga ccctggccac ggtcgtcggg ggcagcttcg
      421 cccgcatcca gttcaccccc gacctgctgc ccgccgacat caccggcacc cgggtctggc
      481 gccagtcctc cgagcgcttc gacgtggagc tgggcccggt gttcgtgaac ttcctcctcg
      541 ccgacgagat caaccgcgcg ccggccaagg tccagtcggc gctgctcgag gtgatggccg
      601 agaagcaggt gtccctcggt ggggagaccc accaggtgcc gagcccgttc ctggtgatgg
      661 ccacgcagaa cccgatcgag tccgagggcg tctacccgct ccccgaggcc cagcgcgacc
      721 gcttcctcat gaagatcctc atcgagtacc cctcgcccgc cgaggagtcc gagatcgtgc
      781 tgcgccacgg catcgacccg cccgaggccg agcgggcgct cgacaccggc gcgctgctgc
      841 gcctgcagga cgccgccgac gccgtctccg tcgaccacgg cacgctgcag tacgccgtcg
      901 acctggtgca ggcgacccgt gacccggccc gctacggctt gcccgacctg gtcgagtacc
      961 tctcctacgg ggcatcgccg cgcgccacgc tcggcctcgt cgccgccggc cgggcgctgg
     1021 ccctgctgcg ccagcgctcc tacgtcgtgc cccaggacgt gttcgacgtg gcgcccgacg
     1081 tcctgcgcca ccgcctcgtc ctgtcctacg agtccctcgc ccagggcgtg ggcgtcgagc
     1141 acgtgctcgc ccgcctgctg tccaccgtcc ccgccccgca cgtggccggc agcccgtccg
     1201 ccagcccgcc ggcgggcgac gccagcgagt gggcggccgg gtgagcacca cggtccacac
     1261 cgaggcggct gaggtcctcc ggcgcctcga gctcgacgtc aaccggcgcc tcgacggcct
     1321 gctgcacggc gactaccgcg gcctggtgcc gggccacggc tccgagaccg gcgagacgcg
     1381 cccctacgtc ccgggcgacg acgtgcgccg catcgactgg aacgtgtcgg cccgctcgat
     1441 ggacacccac gtgcgggagt ccatcgccga ccgcgagctg gagacctggg tcctggtcga
     1501 ccgctccccc agcctcgact tcggcaccgc ccgcaccacc aaggccggcc tggccgtcga
     1561 ggccgtgggg gcgatcggct tcctcacggc tcgcaccggc aaccggctcg gcgcggtcat
     1621 cgtcgacccc gacggcacca ccgtgatccc ggccaagccc gggcgggagc acctcctcgc
     1681 cgtgctgcac ggcctgctca ccggcgcccg gcccggcacc cgcctcggcg gcacctcccg
     1741 ccacgccggc ggcaccaccg acctcggcgc cgccctcggg cacctcatcg tcccgccccg
     1801 acgccgcggt ctcatcgtcg tcgtctccga cttcctgtcc accgacggct ggtcccgcgc
     1861 cctgcggggc ctcacttccc gccacgacgt cgtcgccatc gagatcgt
//
LOCUS       DATISU010000276         4319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_109571, whole genome shotgun sequence.
ACCESSION   DATISU010000276 DATISU010000000
VERSION     DATISU010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109571"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VLR27_10735"
     CDS             complement(<1..633)
                     /locus_tag="VLR27_10735"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HSP03970.1"
                     /translation="MQIAIALFPRLTALDAIGPYEVLQRLPDATVTFVGEQTGEVRSE
                     NGFLGLTVDATFDEVPHPDVVVVPGGVGTRALMAEGSAIHDWLRTAHETSRFTTSVCT
                     GSLVLGAAGLLDGLTATTHWSAYDVLRKTGAEPTGQRVVEHLDRRIITAAGVSSGIDM
                     ALRLAELLVDADAARAMQLMIEYDPEPPFDAGSVEKAGDTVMARLREYSAT"
     gene            complement(674..2227)
                     /locus_tag="VLR27_10740"
     CDS             complement(674..2227)
                     /locus_tag="VLR27_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03971.1"
                     /translation="MHRRTTIVAAVVAAAALLAPAATADTYEDPAYAEFDIENMSRSL
                     GRQAEHLTDPEFHRDFAATAVESQVENAVDQVREAPNGRVYGGLGQVLPGGSVGDPKA
                     YHEVTATEVNFLFRSGAKLEGHLFAPLDGTGPAPAVVITTGSIQGTQHMYWWAARTLA
                     RAGYLVLTWDVQGQGESETTPHAPGQVLPFFDPGSDEGYTDDGVPFQQAYNFHEGTID
                     ALRFLMSTPDDPYVPGGWSAEELAAAHASHDPGSEPIDWVNPLWSAFDRQHIGLAGHS
                     LGAGAVSNAQQCSDAGDRWQEIVDFCTRSFPIRAVVGWDRLQGGDHVPVVPGMDQQAD
                     GYFFNPQPTTEAPTPEQQMERVTGGAFADWRAAGVDTYTVTIRGGVHNEFIEVPYILP
                     GTTYGRDLADHYTLAWMDRYVHPDPARQADAAQRLLAAPIPTTEERESGSALPWRANL
                     MSARYAGAFSFHDVAPTAPAKPGMRVGQTPPPPTLHEAVDLRAYAGLSPVGDWAGANA
                     DRPTVRSAE"
     gene            2365..3060
                     /locus_tag="VLR27_10745"
     CDS             2365..3060
                     /locus_tag="VLR27_10745"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSP03972.1"
                     /translation="MAEMVEFASNGNDARGYLVLPETGTGPGVIVVQEWWGLDSGTKE
                     MADRLAAAGFVALAPDLYHGELAAHDEMDKAGELMQGLPIDRAARDMSGAVDFLADHQ
                     ATTGDGIGVVGFCMGGMLTFVLAAERPDRVKAAVPFYGFPPIEDGPADLSKIEASIQG
                     HMAEHDDFFPPDQAAALEAHLLSMGKDVEITVHEGTGHAFMAPHNALGTQQPEKVAEI
                     WPKVTKFLHDKLG"
     gene            3101..3538
                     /locus_tag="VLR27_10750"
     CDS             3101..3538
                     /locus_tag="VLR27_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03973.1"
                     /translation="MSTSLHPVWLSHHSAHEADRCVRVGGAHVCRRCLAMFVGFVPAV
                     ALLLSPWRDSLQAGDVGLVLALTIAAGLEFAQVARGQMAYSARRVLVLNPFAGMVLAW
                     LGVTGVRDGFSPLHFVLSGVAGALLALLVVRSRTHHGALAQQP"
     gene            3550..>4319
                     /gene="galE"
                     /locus_tag="VLR27_10755"
     CDS             3550..>4319
                     /gene="galE"
                     /locus_tag="VLR27_10755"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HSP03974.1"
                     /translation="MVDPAPTGTVLVTGGAGYIGSHTCVQLLEAGSRVVVVDNLDNSS
                     PVAVDRVRELAGLDPDDPRLTFHELDLRDGPAIEALLRDDPVDSVIHFAGRKAVGESV
                     EQPLAYYDHNLVGTTNLVKAMESAGVRDLVFSSSCTVYGEPDEVPITEAAPVGAINPY
                     GRTKLFIEELLRDVAAAGALEGRSPWRIALLRYFNPVGAHPSGRIGEDPAGIPNNLMP
                     FIMQVAVGRHDRLRVFGDDYDTPDGTCIRDYIHVVDLA"
BASE COUNT          576 a         1532 c         1578 g          633 t
ORIGIN      
        1 ggtggcggag tactcccgca accgggccat gacggtgtcg ccggccttct ccacgctgcc
       61 cgcatcgaag ggcggctccg ggtcgtactc gatcatcagc tgcatcgccc gcgccgcgtc
      121 ggcgtccacc agcagctcgg cgaggcgcag cgccatgtcg atccccgacg acacgccggc
      181 cgcggtgatg atgcgccggt cgaggtgctc gaccacgcgc tggccggtgg gctcggcgcc
      241 ggtcttgcgc agcacgtcgt acgcgctcca gtgggtggtg gcggtgaggc cgtcgagcag
      301 gccggcggcc ccgagcacca gggaaccggt gcacaccgag gtggtgaacc ggctggtctc
      361 gtgggcggtg cgcaaccagt cgtggatggc ggagccctcg gccatcaggg cacgcgtgcc
      421 cacgccgcca ggcacgacga cgacgtcggg gtgggggacc tcgtcgaagg tggcgtcgac
      481 ggtgagcccg aggaacccgt tctcgctgcg cacctcgccg gtctgctccc cgacgaaggt
      541 cacggtggcg tcgggcagtc gctggaggac ctcgtagggg ccgatggcgt cgagcgccgt
      601 gaggcggggg aacagggcga tggcgatctg cacggcgcga tcctgccccc tcagggccgc
      661 cccctcacga agatcactcg gcggagcgca cggtggggcg gtcggcgttg gcgccggccc
      721 agtcgccaac cggggagagg ccggcgtagg cccgcaggtc gacggcctcg tgcagcgtcg
      781 gtggcggtgg ggtctggccg acccgcatgc cgggcttcgc cggtgccgtc ggggcgacgt
      841 cgtggaacga gaacgcgccg gcgtagcggg ccgacatcag gttcgctcgc cacgggaggg
      901 ccgagcccga ctcccgctcc tcggtggtcg ggatcggggc ggcgaggagg cgctgggcgg
      961 cgtcggcctg gcgagccggg tccgggtgca cgtagcggtc catccacgcc agcgtgtagt
     1021 ggtcggccag gtcccggccg taggtggtcc ccgggaggat gtagggcacc tcgatgaact
     1081 cgttgtggac gccaccgcgg atggtgaccg tgtaggtgtc gacgccggcg gctcgccagt
     1141 cggcgaacgc gccacccgtc acccgctcca tctgctgctc cggcgtcggt gcctcggtgg
     1201 tcggctgggg gttgaagaag tagccgtcgg cctgctggtc catgccgggg acgacgggca
     1261 cgtggtcccc gccctgcagc cggtcccatc cgacgacggc ccggatcggg aacgaccggg
     1321 tgcagaagtc cacgatctcc tgccagcgat caccggcgtc ggagcactgc tgcgcgttcg
     1381 acacggcccc ggcgccgagg gagtggccgg cgagtccgat gtgctggcgg tcgaacgcgg
     1441 accagagggg gttcacccag tcgatcggct cgctgccggg gtcatgggac gcgtgggcgg
     1501 cggccagttc ctcggcggac cagcccccgg gcacgtacgg gtcatcgggc gtggacatca
     1561 ggaagcggag ggcgtcgatg gtgccctcgt ggaagttgta cgcctgctgg aaggggacgc
     1621 cgtcgtccgt gtagccctcg tcgctgccgg ggtcgaagaa gggcaggacc tggccgggcg
     1681 cgtgcggcgt cgtctccgac tcgccctggc cctgcacgtc ccacgtgagc accaggtacc
     1741 cggcgcgggc cagcgtgcgg gcggcccacc agtacatgtg ctgggtgccc tggatcgagc
     1801 cggtcgtgat cacgaccgcc ggcgccggtc cggtcccgtc gagcggcgcg aacaggtgcc
     1861 cctcgagctt ggcgccgctg cggaacagga agttgacctc ggtggcggtg acctcgtggt
     1921 acgccttcgg gtcgccgacc gagccgccgg gcagcacctg gccgaggccg ccgtacaccc
     1981 ggccgttcgg ggcctcgcgc acctggtcga cggcgttctc gacctgggac tcgacggcgg
     2041 tggcggcgaa gtcgcggtgg aactcggggt cggtgaggtg ctcggcctgg cgtccgagcg
     2101 agcggctcat gttctcgatg tcgaactcgg cgtaggccgg gtcctcgtag gtgtcggcgg
     2161 tggctgcggg tgcgagcagg gcggcagcgg ccacgacggc ggcgacgatg gtggtgcgac
     2221 ggtgcatggg gttccctccg gtcggccccc tcttcgcccc ggacccccga gaatcccccc
     2281 gcaccccacc ggaaaatccc gtggatccgt cccaccggtg gtgaccgatc cacgagattt
     2341 ccgatcgggg gtaggttccg agccatggct gagatggttg agttcgcgag caacggcaac
     2401 gacgcacggg gctacctggt cctgcccgag acgggcaccg gtccgggcgt gatcgtcgtc
     2461 caggagtggt ggggcctgga ctcgggcacc aaggagatgg ccgaccgcct cgccgcggcc
     2521 ggcttcgtcg cgctggcgcc ggacctgtac cacggtgagc tggccgccca cgacgagatg
     2581 gacaaggccg gtgagctgat gcagggcctg cccatcgacc gcgcggcccg ggacatgagc
     2641 ggcgccgtcg acttcctcgc cgaccaccag gccaccaccg gcgacggcat cggcgtggtg
     2701 ggcttctgca tgggcggcat gctcacgttc gtgctcgccg ccgagcgccc ggaccgggtg
     2761 aaggccgccg tgcccttcta cggcttcccg cccatcgagg acggtcctgc cgacctgtcg
     2821 aagatcgagg cgtcgatcca gggccacatg gccgagcacg acgacttctt cccgccggac
     2881 caggccgccg cgctcgaggc ccacctgttg agcatgggca aggacgtgga gatcaccgtc
     2941 cacgagggca ccggccacgc cttcatggcg ccccacaacg cgctcggcac ccagcagccc
     3001 gagaaggtcg ccgagatctg gcccaaggtc accaagttcc tccacgacaa gctggggtag
     3061 cacgcttcag atggaccgca ctcgtgccga tggagcacgg gtgagcacgt cgctgcaccc
     3121 cgtctggctg tcgcaccact cggcgcacga ggccgaccgc tgcgtccggg tgggcggcgc
     3181 ccacgtctgc cgtcggtgcc tggccatgtt cgtgggcttc gtcccggcgg tggcgctgct
     3241 gctgtcaccc tggcgtgact cgctccaggc cggtgatgtc gggctggtcc tggccctgac
     3301 gatcgcggcg ggcctcgagt tcgcccaggt cgcccggggg cagatggcct acagcgcccg
     3361 ccgggtgctg gtcctcaacc ccttcgccgg catggtcctg gcctggctcg gcgtcaccgg
     3421 cgtgcgcgat ggcttcagcc cgctgcactt cgtcctcagc ggcgtcgccg gggcgctgct
     3481 cgcgctgctc gtcgtccggt cccgcacgca ccacggagcg ctcgcgcagc agccgtaggc
     3541 tcggccgcca tggtcgaccc cgcgcccacc ggcaccgtcc tcgtcaccgg gggcgccggc
     3601 tacatcggct cccacacgtg cgtccagctg ctcgaagccg ggtcgcgcgt ggtcgtggtc
     3661 gacaacctcg acaactcctc accggtcgcc gtcgaccgcg tgcgcgagct cgctggcctc
     3721 gaccccgacg acccccgcct cacgttccac gagctcgacc tgcgcgacgg tcccgccatc
     3781 gaggcgctcc tgcgcgacga cccggtcgac tcggtcatcc acttcgccgg gcgcaaggcg
     3841 gtgggggagt ccgtcgagca gccgctcgcg tactacgacc acaacctggt cggcaccacc
     3901 aacctggtga aggcgatgga gtcggccggc gtccgcgacc tggtgttctc ctccagctgc
     3961 accgtctacg gcgagcccga cgaggtgccg atcaccgagg ccgcgccggt cggggccatc
     4021 aacccctacg gccgcaccaa gctgttcatc gaggagctgc tccgcgacgt cgccgcggcg
     4081 ggggcgctcg agggtcgctc gccctggcgc atcgcgctgc tccgctactt caacccggtg
     4141 ggcgcccatc ccagcggccg catcggagag gatccggccg gcatccccaa caacctgatg
     4201 ccgttcatca tgcaggtcgc cgtgggtcgc cacgaccggc tgcgggtctt cggtgacgac
     4261 tacgacacgc ccgacggcac ctgcatccgc gactacatcc acgtggtcga cctggccga
//
LOCUS       DATISU010000277         1917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_807641, whole genome shotgun sequence.
ACCESSION   DATISU010000277 DATISU010000000
VERSION     DATISU010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807641"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VLR27_10760"
     CDS             <1..1068
                     /locus_tag="VLR27_10760"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSP03975.1"
                     /translation="AANASVEVVLVDGTVDAERAFTVAQRFDEERPEVTVVLHMEPTG
                     DAFARAMQVGVRDILPPGSDADRIRESAKRALELAERRSHTMRSAENAEDQHRLIMVL
                     SPKGGAGKTTIASNLAVGLAMRAPKQVVLVDGDLQFGDVGNALRLLAETTIRDAITGG
                     LHDATEVKVHLTPHRSGLFALCAPEVPGVADEITAQAFSKAVSLLHQEFKYVVVDTDP
                     GLGERTLTVMDNATDMVFVAATDVASVRGLHKTIDALDRIGMTGAKRHFVLNRSNATV
                     GLDVADIAATIGMQPDVLVPSHVTLPVSMNQGVPVIEGGEPSPATAPLWELVDRFEPD
                     PNATAESAAAQRRSSGLFGRR"
     gene            1073..>1917
                     /locus_tag="VLR27_10765"
     CDS             1073..>1917
                     /locus_tag="VLR27_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HSP03976.1"
                     /translation="MRLGDRLKNADGTPAEDAHDDAPAHRADPYARLKHQVSEALMER
                     LAARPSSRDVDDDELLDLARDTLAEIMNQEAVPLTADEKARLVDEISADVIGYGPITP
                     LLEDPDVTEIMCNDVTGVWVERRGLLEETDISFADEAALRRVIDRIVSAIGRRVDESS
                     PMVDARLPDGSRVNAIIPPLAVDGPSLTIRKFSKLVLRSEDYIQTGAATPSLMEFLST
                     CVEAKLNVLVSGGTGTGKTTLLNVLSNFIPLGERIVTIEDAVELRLARRHVVRLESRP
                     QNIEGR"
BASE COUNT          302 a          752 c          606 g          257 t
ORIGIN      
        1 gcggccaacg cctcggtgga ggtcgtcctc gtcgacggca ccgtcgacgc cgagcgtgcc
       61 ttcaccgtgg cccagcgctt cgacgaggag cgccccgagg tcaccgtcgt cctccacatg
      121 gagcccaccg gcgatgcctt cgcccgggcc atgcaggtgg gcgtgcgcga catcctcccg
      181 cccggctccg acgccgaccg gatccgtgag tcggccaagc gagcactcga gctcgccgag
      241 cgccggtcgc acacgatgcg ctcggctgag aacgccgagg accagcaccg cttgatcatg
      301 gtgctgtcgc ccaagggcgg cgccggcaag accaccatcg cgtcgaacct cgcggtcggc
      361 ctcgccatgc gggcgcccaa gcaggtcgtg ctcgtcgacg gcgacctcca gttcggcgac
      421 gtcggcaacg ccctgcgcct gctggccgag accaccatcc gcgacgccat caccggcggc
      481 ctccacgacg ccaccgaggt caaggtgcac ctcaccccgc accgctcggg cctgttcgcc
      541 ctgtgcgccc ccgaggtgcc gggtgtggct gacgagatca ccgcccaggc gttctccaag
      601 gccgtctccc tcctgcacca ggagttcaag tacgtcgtgg tcgacaccga ccccggtctc
      661 ggtgaacgca cgctcacggt gatggacaac gccaccgaca tggtgttcgt cgccgccacc
      721 gacgtggcca gcgtccgcgg cctgcacaag accatcgacg ccctcgaccg catcggcatg
      781 accggggcca agcgccactt cgtgctcaac cggtcgaacg ccacggtcgg cctcgacgtg
      841 gccgacatcg ccgccaccat cggcatgcag cccgatgtgc tcgtcccgag ccacgtcacg
      901 ctgccggtgt cgatgaacca gggcgtgccc gtcatcgaag gcggcgagcc ctcgccggcc
      961 accgcccccc tctgggagct ggtcgaccgg ttcgagcccg accccaacgc gacggcggag
     1021 tccgccgcag cccagcgccg cagcagcggc ctgttcggaa ggcgctagca ccatgcgact
     1081 cggtgaccgg ctgaagaacg ccgacggcac gccggcggag gacgcccacg acgacgcccc
     1141 ggcccaccgc gccgacccct acgcccgcct caagcaccag gtgtccgaag cgctcatgga
     1201 gcgcctcgcc gcccggccca gcagccgcga cgtggacgac gacgagctgc tcgacctcgc
     1261 ccgcgacacg ctggccgaga tcatgaacca ggaggccgtg ccgctcacgg ccgacgagaa
     1321 ggcccgcctg gtcgacgaga tcagcgccga cgtcatcggc tacggcccga tcacgccgct
     1381 gctcgaggat cccgatgtca ccgagatcat gtgcaacgac gtcaccgggg tgtgggtgga
     1441 gcgtcggggc ctgctcgagg agaccgacat cagcttcgcc gacgaggccg cgctccgccg
     1501 cgtgatcgac cgcatcgtgt ccgccatcgg ccgccgcgtt gacgagtcct ccccgatggt
     1561 ggatgcccgc ctgcccgacg gctcccgcgt gaacgccatc atcccgccgc tcgccgtcga
     1621 cggcccctcg ctgacgatcc gcaagttctc caagctcgtc ctgcgctccg aggactacat
     1681 ccagacgggc gcggccacgc cgtcgctgat ggagttcctc tccacctgcg tcgaggcgaa
     1741 gctgaacgtc ctggtcagcg gcggtaccgg caccggcaag acgacgctgc tcaacgtgct
     1801 gtccaacttc atcccgctgg gtgagcgcat cgtcaccatc gaggacgcgg tcgagctccg
     1861 gctcgcccgc cgccacgtgg tccgcctcga gtcccgcccc cagaacatcg agggtcg
//
LOCUS       DATISU010000278        12108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_621991, whole genome shotgun sequence.
ACCESSION   DATISU010000278 DATISU010000000
VERSION     DATISU010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 12108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12108
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621991"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..370
                     /locus_tag="VLR27_10770"
     CDS             26..370
                     /locus_tag="VLR27_10770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSP03977.1"
                     /translation="MDIEIEQTPDYTLCRPSGELDAYTVDEFRNALGGVADAEALLID
                     LSDVPFMDSAGLGALIGGIRRTREAGGEVAVACSRASLTRLLHTTGFDRIVPVAESVD
                     EAAAALAEAQRA"
     gene            complement(372..1454)
                     /locus_tag="VLR27_10775"
     CDS             complement(372..1454)
                     /locus_tag="VLR27_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HSP03978.1"
                     /translation="MSAPDLEQLVRDRRIIVCGGSGGVGKTTTAAVLAMRAAEAGRRA
                     VVVTIDPAKRLADALGLDGLTDAPSQVAGSWPGELWAMMLDTKSTFDALVGKHAADEA
                     QLERILGNRFYKNISGALSGTQEYMAMEKLYELAEETDFDLIVVDTPPTRNALDFLDA
                     PARLSRFLDHRLYRLMTASNRGVLRAVNMATQTFVRTVARVVGADVIDDAVAFFQAFD
                     GMEEGFKRRATRVQQRLRDDDTAFVLVASPRRDTIAEATFFAEKLTEGGIAVRSLIVN
                     RMHPRFDDGLAEATHQRADSLAGTDIGSLYRNLADFQLAASREEENLAGLAEKVAPAP
                     VVRVPFLRSDVHDLEGLRRIEAELFA"
     gene            complement(1451..2452)
                     /locus_tag="VLR27_10780"
     CDS             complement(1451..2452)
                     /locus_tag="VLR27_10780"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HSP03979.1"
                     /translation="MNPTEYFAASRLLIVAGKGGVGKTVVTAALARAASMVGRSALVV
                     EVEGKSGLPRMFGQGTPDQSGLSYDEQLLRRGGGPDGSADVTARTITPDGALLEYLED
                     HGLSRISKRLVTSGALDVVATAAPGIRDILVLGKVKQLQRAGVADVIVLDTPAAGHAI
                     TFLRSAKGLLDAVKVGPINTQARDVLDLLEDHERCQVLLVTLPEETPVNELVETAFSL
                     EDQVGVGLGPVVVNGIYEDLDGLDADPVAAAEQAGVTLRDGEAEVLAEAADFRRHRID
                     LQTEQVQRLSDLLPLPQVRLPFLFESEIGPDEVDSLAQSVLGAIEKLDAEVQLGSPT"
     gene            2530..3741
                     /locus_tag="VLR27_10785"
     CDS             2530..3741
                     /locus_tag="VLR27_10785"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HSP03980.1"
                     /translation="MLALTSLAGSASAQSDPGDGGEETVRILKVSGLIDPVTASFIEE
                     EVEAAADSDVVALVLQVNSEGAVVGDDRMDEVVATIESSPVPVSTWIGPSGSKAEGDA
                     ARLVAASEVSGLSPGSRVEVTSELAEGIDLDGRTAVGERIGSELAAELGVVDNDAPVL
                     GEFIVGLPGVETRQVDDRIEPVTPVRFGQLGLIDQLFHTVASPPVAYLLFVIGASLLV
                     FELFTAGVGVAGMVGVGSLVLSAYGLAVLPTRTWALVLLCLAFPAFAVDVQTGVPRFW
                     TAVGTVALIIGSVAVFEDGPPSWITLLAGIIGVLLAMLGGMPAMVRTRFSTPTIGRDW
                     MIGETGVAVADISPDGVVTVRDAPWRAHTNRATPITAGDEVRVTAIDGLTLEVEPIEG
                     GARDYREARSH"
     gene            3849..4106
                     /locus_tag="VLR27_10790"
     CDS             3849..4106
                     /locus_tag="VLR27_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HSP03981.1"
                     /translation="MTLAIRTETEQWQLKAACRGPQSRVFFPPAHFERKDEKLEREGR
                     AKAICTTCSVQQDCLDYALRIREQHGIWGGLSENERKSLLP"
     gene            complement(4114..5841)
                     /gene="selB"
                     /locus_tag="VLR27_10795"
     CDS             complement(4114..5841)
                     /gene="selB"
                     /locus_tag="VLR27_10795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HSP03982.1"
                     /translation="MHVVATAGHVDHGKSTLVLHLTGTDPDRLAEEKARGLTIDLGFA
                     GATLPSGRRMAMIDVPGHVRFLKNMLAGMGAVDACLFVVAASEGWKPQSEEHLRILDV
                     LGVRHGLIALTKVGLVDDDLHELARLEVEEAVAGTFLEGAPVVGVDAPTGRGLDDLRA
                     ALDDLLAVTPTAVDHGRPRLWIDRSFAPPGAGTVVTGTLAGGSVAVDDELLLAPAGTT
                     VRVRAIQRLHEQVQQLEPGNRTALNLVGVSHGSVARGDVLVRDGQWHHTEQLDASLTV
                     LAGLDHAVSRRGAYLAYIGSGEHPVQLRVLGPQPIEPGETGMVRLHLARHLPLLPGDR
                     YVLRDAGRGETVGGGEVLDVDPVLRASRAAPDRRVERVVAERGWIDADRLALLTGERV
                     EPTIGRWVVSPEARSDAVERVTARLLEAGPLGLDLAALDEQDRAVAERLDDVVVEQGR
                     IVAVDAIDPLDGHPWLDALAAEPFAPPAPPDDLGRNEIRELVRRGAVVEVDGVAFGAD
                     AIEQACDVVRGLLEEHPEGVTVAQIRDGLGTSRKYVLPLLAHLDGTGRTRRRGDLRIA
                     GPRLVAAES"
     gene            complement(5841..7139)
                     /gene="selA"
                     /locus_tag="VLR27_10800"
     CDS             complement(5841..7139)
                     /gene="selA"
                     /locus_tag="VLR27_10800"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HSP03983.1"
                     /translation="MSNPSPAPDRPPSVDQLARSLAGTGLPHPLLVDAAREAIAAGDP
                     GSAQARAEARSRTLLQPVVNATGVLLHTNLGRAPLAASTDGRASNIELDLATGKRGSR
                     TAHAGTLLARAMDAEAAIVVNNCAAAVLLTLAALAGGGRGVAVSRGELVEIGGGFRVP
                     DVMAQSGARLVEVGTTNRTRAADYRDAMDADDDLALLLKVHRSNYTISGFTEDTSVGA
                     LAQLGPPVVVDLGSGLLDAACPWLDHGPPLWLADEPAAKQTLKAGADLVLFSGDKLLG
                     GPQAGVIAGRSDLVRACASHPLYRAFRPGSLVLAALQDVALAYLDRQAGQLPFWRMAA
                     AEVAELEDRARRIVERLDGSVGAEVVACASVPGGGTLPGTEIPSAGVAVAGDRTVELR
                     AGSPPVIARVEDDRTILDLRTVEPADDDVVVAALRDLDGA"
     gene            complement(7252..7911)
                     /locus_tag="VLR27_10805"
     CDS             complement(7252..7911)
                     /locus_tag="VLR27_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03984.1"
                     /translation="MSRTTDRLHRDLGHIADRAVPSPDAWLAIRTRIDGTGHNEMEYV
                     VLSETDAPAPTRRPMVLGAAVAAVVVLVLVAVLVISDDAESTTVADVDAPFVLTIDGE
                     PFVADQLPYCVVNRADASGREAPRQIGGGASGITASGIRFGVSFFSEPVRAGDPEDLE
                     FRGNAVVRRGSDSQTWNAPVEIGAPWSYLTIGPDRVTGHVVMTDLDGETSDVTIDISC
                     P"
     gene            complement(7908..8348)
                     /locus_tag="VLR27_10810"
     CDS             complement(7908..8348)
                     /locus_tag="VLR27_10810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP03985.1"
                     /translation="MTVLEALYRAEYPGMVRLAYTLVGSNAEAEEIVQDSFVEVHGRM
                     CQIRKPGAYLRSAVVSRCRSTLRRRAMAAQRPPDRPASLPSEAGELWDVLGRLPEAQK
                     LTVVLRHYGGYGASEIGELLDMPASTVRSHLRRGLAALRKELER"
     gene            complement(8517..9107)
                     /locus_tag="VLR27_10815"
     CDS             complement(8517..9107)
                     /locus_tag="VLR27_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03986.1"
                     /translation="MMEKVRVAAPRIDCRDLGHIAEGASPSPDAWLPIRTRIGGTGHD
                     EMEHVMMSETEAPAPARRPPILGAPVAAVVALALVAVMMASPAAAHATGVERMENAGW
                     TCIDFAPLGIHCFNEPSSAAFGVEGWPTSVQSRVFTVDDQGHETYQGTEVWIRADVFE
                     RNPKGVRPCPQEGAHWEDLSGFELPYYGCHHFDMHG"
     gene            9354..11537
                     /locus_tag="VLR27_10820"
     CDS             9354..11537
                     /locus_tag="VLR27_10820"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HSP03987.1"
                     /translation="MARFRRTTKLTQLAVALAVVLATPLTAAGVVLATQLFVPLPAVI
                     PEPEELEAIQFSQVYDVNGDLIHTYQQFDQNIPVSPGDIPEVLKQAVIAAEDRNFYGH
                     SGVDIRGTMRALVTDVREGELAQGGSTITQQLAKNAYGVGSDRTIARKVREAVLAAQL
                     DRSLSKEQILFEYLSIVYFGEGAYGIGAAADVYFDKPVNTLNASEAATLAALIPAPSR
                     YNPRADLAVAESRRTRVLDTMLEEEMLTQEEWQAAVDSPLWLASNGPAPGPHTLVVTP
                     TNEDTRYPWFISYLTRYLADALGDEDLLYRGGLQIFTTLDPRAQFAAEAVTFATLSGI
                     DPVVRPTQDGEVTHVMQMAVTAVEPPTGFVRAMVGGRDFGSDQTNIALKGAGSIGRQA
                     GSAFKPFVLATAFEQGIRPNEVYSGGPLTIGDYSPQNYAGAVYGNLTLRSAMHRSVNT
                     TFVRLMQDVGPDEAVAVANRLGLQIPEIDPARDGSGLSIALGSREVSGLGMASAYGTF
                     AHDGIRVPPTPVVRVFAPDGTLLLDRSQPEGEQVLEEATADNVTDVLEGVLTSGTASG
                     KGIGRPAAGKTGTTQDNRDSWFVGYTPTLSASVFMGWDPQPPLDVSYRVKGRALTGGS
                     FPAEAWQEFMRTALEGVPVTDFSEPAPIRDVASEEIQRERRGFAPRAPMFSGSTGVGG
                     PYVVDAEPPVAPAPTTTTTSTTTTSTTTTTLPSGGGLFDDEDDDD"
     gene            complement(11534..>12108)
                     /locus_tag="VLR27_10825"
     CDS             complement(11534..>12108)
                     /locus_tag="VLR27_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HSP03988.1"
                     /translation="AVGPSVFFVSTLDEAGQPSVGSAFVLFSDSERSFLLTSFETVRA
                     ATVEPGPGVEVRQNGTVLSAQLSAWDEGRDLALLVAEDSPNLPAIEVADPSSVGAGDR
                     VFAVSGLGSDGAALVQGVVSDVSATAIQHDAPIGPQFQGGPLLSSSGELVGIASRRFA
                     PLGFLPDTVFFGIPVREACAELVSCPAELD"
BASE COUNT         1757 a         4370 c         4338 g         1643 t
ORIGIN      
        1 ggcaggaacg gtagcgtgcc ccgcgatgga catcgagatc gagcagaccc cggactacac
       61 gctgtgccgc ccgagcggcg agctcgacgc ctacacggtc gacgagttcc gcaacgccct
      121 cgggggcgtg gccgacgccg aagcgctcct catcgacctc tccgacgtcc ccttcatgga
      181 ttcggccggg ctcggcgccc tcatcggcgg catccgccgc acccgcgagg ccggcggcga
      241 ggtcgccgtg gcctgctcgc gtgcctcgct cacccgcctg ctgcacacca ccggcttcga
      301 ccggatcgtg ccggtggccg agtcggtcga cgaggcggcc gccgccctcg ccgaggccca
      361 gcgcgcctag ctcaagcgaa gagctcggcc tcgatgcggc gcaggccctc gaggtcgtgc
      421 acgtcggagc gcaggaacgg cacccgcacc accggtgccg gagccacctt ctcggccagg
      481 cccgccaggt tctcctcctc gcgggaggca gccagctgga agtcggccag gttccggtag
      541 agcgagccga tgtcggtgcc ggccagggag tcggcgcgct ggtgcgtggc ttcagccagc
      601 ccgtcgtcga agcgggggtg catccggttg acgatgagcg accgcacggc gatgccgccc
      661 tcggtgagct tctcggcgaa gaacgtggcc tcggcgatgg tgtcgcgccg cggtgaggca
      721 acgagcacga acgcggtgtc gtcgtcgcgg aggcgctgct gcacccgcgt cgcccggcgc
      781 ttgaacccct cctccatgcc gtcgaaagcc tggaagaagg ccacggcatc gtcgatgacg
      841 tcggcaccga cgacgcgggc gacggtgcgc acgaaggtct gcgtggccat gttcacggcc
      901 cgcagcacgc cgcggttgga cgccgtcatc aaccggtaga ggcggtggtc gaggaagcgg
      961 ctgagccggg ccggggcgtc gaggaagtcg agcgcgttcc gggtgggtgg cgtgtcgacg
     1021 acgatgaggt cgaagtcggt ctcctcggcc agctcgtaga gcttctccat ggccatgtac
     1081 tcctgcgtgc cggagagggc gccggagatg ttcttgtaga agcggttgcc gaggatgcgc
     1141 tcgagctggg cctcgtcggc cgcgtgcttg cccaccagcg cgtcgaaggt cgacttggtg
     1201 tcgagcatca tcgcccacag ctcgcccggc cacgaccccg ccacctggct gggcgcatcg
     1261 gtgagcccgt cgaggccgag ggcatcggcg agccgcttgg ccggatcgat ggtgaccacg
     1321 acggcgcgcc gcccggcctc cgcggcccgc atggcgagga cggctgcggt ggtggtcttg
     1381 ccgacgccac cggagccgcc gcacacgatg atgcgccgat cgcggaccag ctgctcgagg
     1441 tcgggggcgc tcacgtgggc gaccccagct ggacctcggc gtcgagcttc tcgatggccc
     1501 ccagcaccga ctgggcgagg gagtccacct cgtccgggcc gatctcggac tcgaacagga
     1561 acggcaggcg cacctggggc agcgggagca ggtcggacag gcgctgcacc tgctcggtct
     1621 gcaggtcgat gcggtgccgg cggaagtcgg ccgcctcggc caggacctcg gcctccccgt
     1681 cgcggagggt gaccccggcc tgctcggcgg cggccaccgg atcggcgtcg aggccgtcga
     1741 ggtcctcgta gatgccgttg accacgaccg ggccgagacc caccccgacc tggtcctcca
     1801 gggagaacgc cgtctcgacc agctcgttca ccggggtctc ctccgggagg gtgacgagga
     1861 ggacctggca gcgctcgtgg tcctccagca ggtcgagcac gtcgcgcgcc tgggtgttga
     1921 tcggaccgac cttcaccgca tcgagcaggc ccttggccga ccgcaggaac gtgatggcgt
     1981 gcccggccgc cggggtgtcg aggacgatga cgtcggcgac gccggcgcgc tggagctgct
     2041 tgaccttgcc gagcacgagg atgtcccgga tgccgggtgc cgcggtggcc acgacgtcga
     2101 gcgcgccgct ggtgacgagc cgcttggaga tgcgggacag gccgtggtcc tcgaggtact
     2161 ccagcagcgc accgtccggt gtgatcgtgc gagcggtgac gtcggcggac ccgtccggac
     2221 cccctccccg gcgcaggagc tgctcgtcat agctgagtcc ggactggtcc ggcgtaccct
     2281 gtccgaacat ccggggcagg cccgacttgc cctcgacctc caccaccagc gcggagcgac
     2341 cgaccatcga cgccgccctg gccagggctg cggtgacgac cgttttgccc acgccaccct
     2401 tgcccgcaac gatgagcagc cgggatgcag cgaagtactc cgtcggattc accgaaacac
     2461 cacgtccatc ctcctcgtcg ccgagcgcga gccaccctac ccagggtcat cgcgggcctc
     2521 gcgctgtgcg tgctggccct caccagcctg gcgggcagcg cgtccgcgca gtcggacccg
     2581 ggcgacgggg gcgaggagac ggtccgcatc ctcaaggtct cgggcctgat cgacccggtc
     2641 acggcctcct tcatcgagga ggaggtcgag gcggccgccg acagcgatgt cgtcgccctc
     2701 gtcctgcagg tcaacagcga gggtgccgtc gtcggtgacg accggatgga cgaggtcgtt
     2761 gcgacgatcg agtcctcgcc ggtccccgtg tccacctgga tcggcccgtc cggctcgaag
     2821 gccgaaggcg acgccgcccg cctggtcgcg gcctccgagg tctccggcct ctcccccggc
     2881 agccgcgtcg aggtcacgag cgagctggcc gagggcatcg acctcgacgg gcgcaccgcc
     2941 gtgggcgagc gcatcggcag cgagctggcc gccgagctcg gtgtcgtcga caacgacgcc
     3001 ccggtcctcg gcgagttcat cgtcgggctc cccggcgtgg agacccgcca ggtcgacgac
     3061 cgcatcgaac cggtcacccc ggtccgcttc ggccagctcg gcctcatcga ccagctgttc
     3121 cacaccgtgg ccagcccgcc ggtggcctac ctcctcttcg tgatcggcgc gagcctgctc
     3181 gtcttcgagc tgttcaccgc cggcgtcggc gtggccggca tggtcggcgt cgggtcgctc
     3241 gtgctgtccg cctacggcct cgccgtgctg ccgacccgca cctgggcgct ggtgctgctc
     3301 tgcctggcgt tcccagcctt cgccgtcgac gtccagacgg gtgtgccgcg cttctggacc
     3361 gcggtcggca cggtcgcctt gatcatcggc tcggtcgccg tcttcgagga cggtcctccg
     3421 tcgtggatca ccctgctcgc cggcatcatc ggcgtgctgc tggccatgct cggcggcatg
     3481 ccggccatgg tgcgcacccg gttctcgacc cccaccatcg ggcgcgactg gatgatcggc
     3541 gagaccggag tcgccgtcgc cgacatctcc ccggacggcg tcgtcaccgt gcgcgacgcc
     3601 ccgtggcggg cccacaccaa ccgggccacc cccatcaccg ccggcgacga ggtccgggtc
     3661 acggccatcg acggcctcac cctcgaggtc gagcccatcg agggcggcgc ccgggactac
     3721 cgcgaggccc gcagccactg atccgacgct gctcgcgtcc gcgagcagaa cgctcgcgct
     3781 gattcacccc ttgtgcagaa cgacctgcgg acctaggttc ggcgccacgt cgacaccggg
     3841 gggttcgcat gacgctggcg attcgcacag agaccgagca gtggcagctc aaggctgcct
     3901 gtcgggggcc gcagtcacgg gtcttcttcc cgccggcgca cttcgagcgc aaggacgaga
     3961 agctcgagcg cgagggacgg gcgaaggcga tctgcaccac ctgctccgtg cagcaggact
     4021 gcctcgacta cgcgctgcgc atccgtgagc agcacggcat ctggggcggc ctgagcgaga
     4081 acgagcgcaa gtcgctgctc ccgtagcgac cagctagctc tccgcagcaa ccaagcgagg
     4141 cccggcgatc cgcagatcgc cgcgccgtcg cgtccggccg gtgccgtcga ggtgggcgag
     4201 cagcggcagc acgtacttgc ggctggtgcc gaggccgtcg cggatctggg cgacggtgac
     4261 cccctcgggg tgctcctcga gcagcccccg caccacatcg caggcctgct cgatggcgtc
     4321 ggcgccgaag gccaccccgt cgacctccac caccgcaccg cgccggacca gctcgcggat
     4381 ctcgttgcgg ccgaggtcgt caggcggcgc cggcggtgcg aacggctcgg cggcgagcgc
     4441 gtccagccac gggtggccgt ccagcgggtc gatggcgtcg acggccacga tgcggccctg
     4501 ctcgaccacg acgtcgtcga ggcgctcggc cacggcgcgg tcctgctcgt cgagcgcagc
     4561 caggtcgagc ccgagcggcc ccgcctccaa caaccgggcg gtgacccgct cgacggcgtc
     4621 ggaccgggcc tcgggggaga ccacccagcg gccgatcgtg ggctcgaccc gctccccggt
     4681 cagcagcgcg aggcggtcgg cgtcgatcca gccccgctcg gccaccacgc gctccacccg
     4741 tcggtcgggg gcagcccgcg acgcgcgcag caccgggtcg acgtcgagca cctcaccgcc
     4801 gcccacggtc tcgccgcgac cggcgtcgcg cagcacgtag cggtcgcccg gcagcagcgg
     4861 caggtggcgg gccaggtgga ggcggaccat gcccgtctcc cccggctcga tcggttgggg
     4921 cccgaggacg cgcagctgga ccgggtgctc gccggagccg atgtaggcca ggtacgcgcc
     4981 ccgccgggac acggcgtggt cgaggccggc cagcacggtg agcgacgcgt cgagctgctc
     5041 ggtgtggtgc cactgcccgt ctcggacgag cacgtcgccc cgggccaccg agccgtggct
     5101 cacgccgacg aggttgagcg ccgtgcggtt ccccggctcg agctgttgga cctgctcgtg
     5161 caggcgctgg atggcccgca cccgcacggt ggtgccggct ggtgcgagga ggagctcgtc
     5221 gtccacggcg accgacccgc cggcgagggt gccggtgacg accgtgccgg ccccgggcgg
     5281 ggcgaaggac cggtcgatcc agagtcgcgg gcgaccgtgg tcgacggcgg tcggcgtgac
     5341 ggccagcagg tcgtcgaggg cggcccgcag gtcgtccaga ccccgtccgg tcggtgcgtc
     5401 gaccccgacc acgggcgccc cctcgaggaa cgtgccggcc accgcctcct cgacctcgag
     5461 tcgggcgagc tcgtggaggt cgtcgtcgac gagaccgacc ttcgtcaggg cgatgaggcc
     5521 gtggcggacg ccgagcacgt cgaggatgcg caggtgctcc tcgctctggg gcttccaccc
     5581 ctcgctggcg gcgaccacga acaggcacgc gtccaccgca cccatgccgg ccagcatgtt
     5641 cttgaggaac cgcacgtggc ccgggacgtc gatcatcgcc atgcgccggc cggacggcag
     5701 ggtggcgccg gcgaagccga ggtcgatggt gagcccgcgc gccttctcct cggccagtcg
     5761 gtcgggatcg gtgccggtga ggtgcaggac gagggtggac ttgccgtggt cgacgtgccc
     5821 ggcggtggcc acgacgtgca tcaggcgccg tccaggtcgc gcagggcggc cacgacgacg
     5881 tcgtcgtcgg ccggctcgac cgtgcgcagg tcgaggatgg tgcgatcgtc ctccacccgg
     5941 gcgatgacgg gcggggaccc ggcgcgcagc tccacggtgc ggtcgccggc caccgccacg
     6001 ccggcggagg ggatctcggt gccgggcagc gtgcctccgc cgggcaccga cgcgcacgcg
     6061 acgacctcgg caccgaccga gccgtccagc cgttcgacga tgcggcgggc ccggtcctcc
     6121 agctcggcca cctcggcggc tgccatccgc cagaagggca gctgcccggc ctgccgatcg
     6181 aggtaggcca gcgccacgtc ctggagggcg gccagcacga gcgagcccgg ccggaaggcg
     6241 cggtagaggg ggtggctggc gcaggcgcgc accagatccg accggccggc gatgacgccg
     6301 gcctgcgggc cgccgaggag cttgtcgccg gagaacagga ccaggtcggc ccccgccttg
     6361 agcgtctgct tggcagcggg ctcgtccgcg agccacaggg gcgggccgtg atcgagccac
     6421 gggcaggccg cgtccaacag gccgctgccg aggtcgacga ccaccggtgg cccgagctgg
     6481 gcgagcgcgc ccacggacgt gtcttcggtg aagccggaga tcgtgtagtt cgagcggtgg
     6541 accttgagca ggagcgcgag gtcgtcgtcg gcgtccatgg catcgcggta atcggcggca
     6601 cgggtgcggt tggtggtgcc gacctcgacg agtcgggcgc cggactgggc catcacgtcg
     6661 ggcacccgga agccgccacc gatctccacc agctcccccc ggctgacggc gacgccccgt
     6721 ccgccaccgg ccagcgcggc gagggtgagc agcacggcgg cggcgcagtt gttcacgacg
     6781 atcgccgcct cggcgtccat cgcccgggcc aggagggtgc cagcgtgggc ggtgcggctc
     6841 ccccgcttcc cggtggcgag gtcgagctcg atgttggacg cccgcccgtc ggtgctggcg
     6901 gcgagcggcg cgcgcccgag gttcgtgtgc aggaggacgc ccgtggcgtt caccaccggt
     6961 tgcagcagcg tgcgggaccg cgcctcggcc ctggcctgcg ccgagccggg gtcgcccgcg
     7021 gcgatcgcct cgcgtgcagc gtcgaccagc agcggatggg gcaggccggt gccggcgagg
     7081 gaccgggcca gctggtcgac cgacggtggc cgatcgggag cgggcgacgg gttcgacacg
     7141 gccgccgact ctaggacgag gtcggctgcg cccatccccg gtcgtcacgc cagacgatcg
     7201 gttcaggtga gccgcgcaag accggcggtc cgccatccgc tgtgccagga gctacgggca
     7261 gctgatgtcg atcgtgacgt cgctcgtctc gccgtcgaga tcggtcatga ccacatggcc
     7321 ggtgacgcgg tccgggccga tggtcaggta cgaccacggc gcgccgatct ctactggagc
     7381 gttccaggtc tgcgaatcgg acccacgacg gacgacggcg ttgcctcgga actcgaggtc
     7441 ctcgggatcg ccggcgcgca ccggctcgct gaagaagctg acgccgaagc ggatgccgga
     7501 ggcggtgatg cccgaggcgc cgcccccgat ctgccgcggt gcctcccggc cggatgcatc
     7561 ggcgcggttg acgacgcagt aggggagctg atcggcgacg aagggctcgc cgtcgatggt
     7621 gagcacgaac ggtgcatcca cgtctgccac ggtcgtggat tcggcgtcgt cgctgatgac
     7681 gagcaccgcg accagtacga ggaccacgac cgcggcgact gccgctccca ggaccatcgg
     7741 tcggcgcgtc ggggcaggtg cgtcggtctc ggagagcacg acgtactcca tctcgttgtg
     7801 ccctgtgccg tcgatccggg tgcggatcgc gagccaggca tcgggtgacg gcacggcgcg
     7861 gtcggcgatg tgcccgagat cccggtgcag tcggtcggtg gtgcggctca tcgctccagc
     7921 tccttgcgca acgcagccag gccacggcgg aggtgcgagc gcaccgtcga cgctggcatg
     7981 tcgagcagct cgccgatctc cgacgccccg taaccgccgt agtggcggag cacgaccgtc
     8041 agcttctgcg cctcgggcaa ccggccgagc acatcccaga gctcgcccgc ctcgctcggc
     8101 agcgacgccg gccggtcagg tggacgctgg gccgccatgg cccggcgtcg caacgtcgac
     8161 cggcaccggc tgaccaccgc actgcggagg taggcgcctg gcttgcggat ctgacacatc
     8221 cggccgtgga cctcgacgaa gctgtcctgc acgatctcct cggcctcggc gttgctgccg
     8281 accagcgtgt acgccaaccg caccatcccc gggtactccg cgcggtacag cgcctcgagg
     8341 accgtcaccg acgacggtgc ctgatcctct gattgcgcag tccgcacctt ctccatcacc
     8401 ccctacggcg aatggtctca ctgaaacgtt gcagggcata ggagaacgtc cttctccgac
     8461 gaggacgccg tgccgagtgg acctgagcga ccgacggcag ggcgtgctgg gccccgtcag
     8521 ccgtgcatgt cgaagtgatg gcagccgtag tagggcagct cgaacccgga caagtcttcc
     8581 cagtgggcac cttcctgcgg gcacggacgg actcccttcg ggttgcgctc gaacacgtcg
     8641 gcccggatcc acacctcggt gccctgatag gtctcgtgcc cctgatcgtc gaccgtgaag
     8701 acacgggact gcacactggt cggccacccc tcgacgccga acgcggccga tgacggttcg
     8761 ttgaagcagt ggatcccgag aggagcgaag tcgatacagg tccaccccgc gttctccatc
     8821 cgctcgaccc ccgtggcgtg cgctgcggcc ggcgacgcca tcatgacggc gaccagcgca
     8881 agcgccacga cagcggcgac tggcgctcca aggatcggcg gtcggcgggc cggggcaggt
     8941 gcttcggtct cggacatcat gacgtgctcc atctcgtcgt ggcctgtgcc gccgatccgg
     9001 gtgcggatgg ggagccaggc atcgggtgat ggagaggcgc cctcggcgat gtggccgagg
     9061 tcgcggcagt cgatccgtgg tgcggcaacc cgcaccttct ccatcacccc ctacggcgaa
     9121 cggtctcagc ggaacgttgc agtgcacggg agaacgcccc ttcgggagcg acaccacgaa
     9181 ccgccgggcg aagccaactc atcggcaaca gcggccgagc gagagtgcag ggacccgcgc
     9241 gttcgagcgc actccaggcg ggggctgttc gcatcatcag aggccgactg aacccgtgaa
     9301 cgtgcgcgct ccgatgcgac atccggcgcg aatcgggcga tgatggtggg cgcatggctc
     9361 ggttccgccg cacgaccaag ctgacccagc tcgccgtcgc cctcgccgtg gtgctggcca
     9421 cgccgctcac cgcggccggc gtcgtgctgg ccacccagct gttcgtgccg ctcccggcgg
     9481 tcatccccga gcccgaggag ctggaggcga tccagttcag ccaggtgtac gacgtcaacg
     9541 gcgacctgat ccacacctac cagcagttcg accagaacat cccggtgtca cccggcgaca
     9601 tccccgaggt gctgaagcag gcggtgatcg cggccgagga ccgcaacttc tacggccact
     9661 ccggtgtgga catccgcggc acgatgcgtg ccctggtcac cgacgtgcgg gagggcgagc
     9721 tcgcccaggg cgggtccacc atcacccagc agctggccaa gaacgcgtac ggcgtcggca
     9781 gcgaccgcac catcgcccgc aaggtgcgcg aggccgtgct ggccgcccag ctcgaccggt
     9841 ccctgtcgaa ggagcagatc ctcttcgagt acctgtccat cgtctacttc ggcgaagggg
     9901 cctacggcat cggcgcagcg gccgacgtgt acttcgacaa gccggtgaac accctcaacg
     9961 cgtcggaggc ggcgacgctg gcggcactca tcccggcccc gtcgcggtac aacccgcggg
    10021 ccgacctggc cgtcgcggag agccggcgca cccgcgtgct ggacaccatg ctcgaggagg
    10081 agatgctgac ccaggaggag tggcaggcgg ccgtggactc ccccctgtgg ctggcgagca
    10141 acggcccggc gcccgggccg cacacgctgg tggtcacccc gacgaacgag gacacccgct
    10201 acccctggtt catctcgtac ctcacccggt acctggccga cgcgctcggc gacgaggacc
    10261 tgctgtaccg aggcggcctg cagatcttca ccacgttgga ccccagggcc cagttcgccg
    10321 ccgaggccgt cacgttcgcc acgttgagcg gcatcgaccc ggtcgtgcgc cccacccagg
    10381 acggcgaggt cacccacgtc atgcagatgg cggtgacggc cgtcgagccg ccgaccggct
    10441 tcgtgcgggc catggtcggc ggccgggact tcggctccga ccagaccaac atcgcgctca
    10501 agggggccgg gagcatcggc cgccaggccg gctccgcgtt caagccgttc gtgctcgcca
    10561 ccgcgttcga gcagggcatc cgcccgaacg aggtgtactc cggcggtccg ctcaccatcg
    10621 gcgactactc gccgcagaac tacgccggcg ccgtgtacgg gaacctgacc ctgcgatcgg
    10681 cgatgcaccg ctcggtcaac accaccttcg tccgcctcat gcaggacgtg ggacccgacg
    10741 aggctgtggc cgtggccaac cggctcgggc tgcagatccc cgagatcgat ccggctcggg
    10801 acggctccgg tctgtccatc gccctcggca gccgggaggt ctccgggctg gggatggcat
    10861 cggcgtacgg cacgttcgcc cacgacggca tccgcgtgcc gccgaccccg gtggtgcgag
    10921 tgttcgcccc ggacgggacc ctcctgctcg accgatccca acccgagggc gagcaggtgc
    10981 tggaggaggc caccgccgac aacgtcaccg acgtgctcga gggcgtgctc acgtccggca
    11041 ccgcgtccgg caagggcatc ggtcgcccgg cggcgggcaa gaccggcacc acccaggaca
    11101 accgcgactc ctggttcgtc gggtacacgc cgacgctgtc ggcatcggtg ttcatgggct
    11161 gggacccgca accgccgctc gacgtcagct accgggtgaa gggccgagcg ctcacgggcg
    11221 ggtcgttccc agccgaggcg tggcaggagt tcatgcgcac cgcgctcgag ggcgtgccgg
    11281 tcaccgactt cagcgaaccg gcgccgatcc gcgacgtggc cagcgaggag atccagcgcg
    11341 agcggcgggg cttcgcaccc cgggcgccga tgttctcggg ttcgaccggc gtcggaggcc
    11401 cctacgtcgt cgacgccgaa ccacccgtgg caccggcccc gacgacgacc accacctcca
    11461 cgaccaccac gtcgaccacg acgacgacgc tgccgtcagg cggcggcctc ttcgacgacg
    11521 aggacgacga cgactaatcc agctccgccg ggcacgagac gagctcggcg cacgcctccc
    11581 ggaccgggat cccgaagaag acggtgtcgg ggaggaagcc gagcggggcg aagcgccgcg
    11641 acgcgatgcc cacgagctcg ccggaggagc tcagcagcgg tccgccctgg aactgcgggc
    11701 cgatcggcgc atcgtgctgg atggcggtgg ccgacacgtc ggacacgacg ccctggacga
    11761 gggcggcgcc gtcgctgccc agacccgaca ccgcgaagac ccggtcaccg gcgcccaccg
    11821 aggacgggtc ggcgacctcg atcgccggca ggttcggcga gtcctcggcg acgagcaggg
    11881 cgaggtcccg cccttcgtcc caggcgctga gctgcgccga caggaccgtg ccgttctggc
    11941 gcacctcgac gcccgggccc ggttcgaccg tggcggcccg caccgtctcg aacgaggtga
    12001 gcaggaagga gcgctcgctg tcggagaaca gcacgaacgc ggacccgacg gagggttgcc
    12061 cggcttcgtc gagggtgctc acgaagaaga cggacggccc gacagcag
//
LOCUS       DATISU010000279         9408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_389646, whole genome shotgun sequence.
ACCESSION   DATISU010000279 DATISU010000000
VERSION     DATISU010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389646"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..833)
                     /locus_tag="VLR27_10830"
     CDS             complement(198..833)
                     /locus_tag="VLR27_10830"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HSP03989.1"
                     /translation="MRDPLLDPPSWAPAWLPTVDDVRGARLLRWLVTAVFVAGVAACV
                     TEGANSPADPELGPVAAETTTSAPAGGLAERFGTVAAQLVALSGEVLELCLLHADTAE
                     ERATGLMQVTDLEGHDGMLFSMEEPREGAFYMYRTLLPLTIGWWDAEGAFVSRADMVP
                     CESEDEAACERYPAAGPFRFAIEVVQGDPLAARFEPGARLQVGTEACAVPA"
     gene            complement(890..1765)
                     /gene="panB"
                     /locus_tag="VLR27_10835"
     CDS             complement(890..1765)
                     /gene="panB"
                     /locus_tag="VLR27_10835"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HSP03990.1"
                     /translation="MRTSWNAPSIRAVKTTDGTSAQPLVMVTAYDAPGAKMAHAAGVD
                     LILVGDSVAMVVLGHDDTLSVTVDDIAHHTGAVARGLKASTAEGRLPMVVADLPWMSY
                     HTDTHEAVRNAAALVRAGAHSVKLEGGRKRLPVIEAILDAEIPVMGHLGLTPQSVHAM
                     GGFKVQGKHHDAAMELVSDAKALAAAGCFAIVLEGVPDAVARMVTDAVDVPTIGIGAG
                     RDCDGQVLVFHDVLGIEDRMAPKFVRRYAEIGSASVDALSAFAADVRSGAFPNDDESY
                     HLSAEAAETLGLYGS"
     gene            1891..2061
                     /locus_tag="VLR27_10840"
     CDS             1891..2061
                     /locus_tag="VLR27_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03991.1"
                     /translation="MAIGTCDSCGSEEDVTEVRRVYVTPEDWDTEGKAEPQPGTEMWC
                     YACRAHYPHQEV"
     gene            complement(2074..4326)
                     /locus_tag="VLR27_10845"
     CDS             complement(2074..4326)
                     /locus_tag="VLR27_10845"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HSP03992.1"
                     /translation="MAPPATPSPSRKPKAKPAPKRRSVLWRFRRSLFLGALLFVGSIA
                     GAGFVLAQVDVPDADPLLQSTFVCAADVTDGCSADNAMAKLSGEEDRVSVPLEEMPQI
                     FIDAVLAAEDRQFFSHSGIDPLGIARAVYRDFKGDGVQQGGSTITQQYAKNAFLSSER
                     TITRKLKEAVLAVKLERDLSKQEILQRYLNTVYFGRGAYGAEAAARAYFGVSIRNVDL
                     AQAAYLAGLVRAPESADATTEPERATFRRDSVLAGMIVTGAITQAERDEAAAVPIGDM
                     VQPRAARDGVSMVKGADVGATYYVEYVRRLLYEEYGADVVNGGGLRVYTALDLGLQGE
                     ANHAVWDTLNEPDDPSGALVSVDDVGRVVAMVGGKDFDASEVNLALGAAAGGSGRQPG
                     SSFKPIVLATALEQGISLNSQFRNVYERTFPEANAGEDWEVTNYARGNEDVIDLVEAT
                     TVSSNTVYADLMIEVGPGNAVDVARKLGVRSELPAVNSLVLGSGEVSVLDMASAYSSF
                     AREGESIEPIFITKVEQVVDGEVKVLSTSAARTQRAVSESTAAQVAWTLRQNVLSGTG
                     TGASIGVPAAGKTGTTQDYRDAWFVGFTPQLTTAVWMGYPDVDEDGQPRFMRNVRGRD
                     VTGGSFPAQIWRTFMQAAVAGEDYGDFPKPTFTGEPLNPDLTTTTEAPPSTRATTTTD
                     PDPTTTEPDDDEEDEEEDDTTTTSQPRSTTTAPPSTTTTTAAPTTTAAPTSTTTTTRP
                     GGGGTIIDGD"
     gene            complement(4259..5503)
                     /locus_tag="VLR27_10850"
     CDS             complement(4259..5503)
                     /locus_tag="VLR27_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03993.1"
                     /translation="MTALLLLLLAAPVVGAAVAFVLPHHATRACRSIAAGVAVGWVVL
                     AAQDAPVTWGDLVTDPLLAAAAAGTALLVVATRPHSATAAGAALLILTLLPAAAAIDP
                     DRLPDRRLAAGVVLVAVLAAARLWTERAPRLGQLLTLLAGVVIATGLIGDDPAEAVAL
                     AVAGTTVAVVAAAVWGAPGRLLLPAGLLAVSRAAPLREAADGTDWTLIVVAALVAVAA
                     VVLRAVRSRALTERLPLAATAVAAALLAGDVGELRSAGALLGAGAVLALAGRHPLALL
                     ALLPGTVAAIDVIGLADQPAHAAVGAAIIAVVAVTSTGPLAPVDDRSRPGLLAAVAVA
                     FAVVPTWGWAGVDLAEYRTSLAVVVAVALPVLLLGTPAWRERRPAGGTIGARPFPGFS
                     HGTARHPEPVPEAQGQAGAQAP"
     gene            complement(5500..6123)
                     /locus_tag="VLR27_10855"
     CDS             complement(5500..6123)
                     /locus_tag="VLR27_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP03994.1"
                     /translation="MSAAAPGLAAPGPPTSLQPTSVAVATAATLAAAALAVTSTGSMV
                     LAALLVGVAARDRRSAGAALLAVVATAIRFRTATFDDLAGIQSVLGPAGTVGPATAAA
                     SAWLAAAAVILAARPLVRPSLQAATGRLDRLRDATPALACGLFAAALVVGPGPSDLGV
                     RVLASVVGVALAAGVTFTDARTAFGRARPWAALVAGVAAAVLAGWPS"
     gene            complement(6120..6530)
                     /locus_tag="VLR27_10860"
     CDS             complement(6120..6530)
                     /locus_tag="VLR27_10860"
                     /inference="COORDINATES: protein motif:HMM:NF036456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HSP03995.1"
                     /translation="MPFHPGALSKGVGVARPAGVVDHRIARNALVAEYERGRLARHEV
                     CDAHPELRRAAEHISRKTTQICPICEDRFLVLVTYAFGPHLPKSGYAIEDRADMDKVR
                     RTTTECTCYVIEVCVGCGWNHLLQTFPVEGFRGT"
     gene            complement(6577..7287)
                     /locus_tag="VLR27_10865"
     CDS             complement(6577..7287)
                     /locus_tag="VLR27_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSP03996.1"
                     /translation="MAWTPFTVAAPNGADLPDDAVLYGPEIPGEPVLRLLGDLSGKRV
                     LDLGCGAGRNAIAMARAGARVIAVDEDAEQIGAARRAIDAADMKVELHHAPLAELAFL
                     RADTIDAAISVHSLVQVADVGRVFRQIHRVLRPEHSFVVSVPHPTFALLDPDGPDPLQ
                     VRRSAFDEQPYAPGDPRHGTHARSTGALFTELTRAGFRVDHVLEPAPTGAGHGPHWSP
                     AMEKVPATLILRARKEGI"
     gene            complement(7354..8760)
                     /locus_tag="VLR27_10870"
     CDS             complement(7354..8760)
                     /locus_tag="VLR27_10870"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HSP03997.1"
                     /translation="MIPERFAPVLADVAPLAQAFDAAGHRLYLVGGVVRDLLLGDDRP
                     VDYDFTTDAPPDRIEEILGSFAGTEALWTQGKRFGTIGAKVRVGEGDDARVVDHEITT
                     HRAEAYVPDSRKPEVSFATDIEADLSRRDFTINAMALSLPDARLVDPFGGTADLAAKV
                     LRTPLSASESFSDDPLRMLRAARFLTRHDLKPDDELVEAVEQMGDRIAIVSPERIRDE
                     LNRLVVLDDPSPGLWFLADTGLSEHFLPELSAMRLEQDPIHHHKDVLAHTIAVVRKTS
                     PDLVLRLAALFHDIGKPDTRDFGPKGVTFHHHEVVGARMTRKRMKELKYPNEIRDDVS
                     QLVFLHLRFHGYGDDAWTDAAVRRYVRDAGPLLDKLNELTRCDCTTRNRRKAEMLARR
                     MDALEERIAALREQEAIDAIRPDLDGQQVMDLLDVRPGPVVGQALRMLLEVRMEEGPL
                     GEDEARRRVLAWWAKRSS"
     gene            complement(8866..>9408)
                     /locus_tag="VLR27_10875"
     CDS             complement(8866..>9408)
                     /locus_tag="VLR27_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSP03998.1"
                     /translation="SVGERLDRFAEACAIVRSLSTQERTTFHGEHYHLTDAPMEPKPV
                     GPFPLLVGGGGERRTLRIAAEHAQEWNVWSTPEIFAHKSGVLDGHCQDLGRDPAEIHR
                     STQALLFLSDDQAFLDRMRGMDMPMPTLIGTPSELVDVVGAYREAGVDELIVPDFTIG
                     TGSQRTDILDRFKEEVAIHF"
BASE COUNT         1281 a         3164 c         3747 g         1216 t
ORIGIN      
        1 accatcaatt cataggcgcg catgcctacg gacaccttcc tgtggatccg gcagggccgg
       61 agcccccgcg gcgcgggagc aggacggaca agggaactgc acatcgtatc gacggagctg
      121 gtcgtgggca catccacgtc ccccagcatc cacgaggggg gtgacaagaa cgcgcggacc
      181 ggcgcgcggt ggggcggtca ggccgggacg gcgcaggcct cggtgcccac ctgcagccgg
      241 gcgccgggct cgaagcgggc ggcgagcggg tcgccctgca ccacctcgat cgcgaaccgg
      301 aacgggccgg ctgccgggta ccgctcgcag gcggcctcgt cctcggactc gcaggggacc
      361 atgtcggccc gggacacgaa cgcgccctcc gcgtcccacc acccgatcgt gaggggcagc
      421 aaggtccggt acatgtagaa ggcgccctcg cggggctcct ccatgctgaa cagcatcccg
      481 tcgtggccct cgaggtcggt cacctgcatg aggccggtgg cgcgctcctc ggccgtgtcg
      541 gcgtggagca ggcacagctc gagcacctca cccgacagcg ccacgagctg ggcggcgacc
      601 gtgccgaacc gctcggcgag accgccggcc ggcgccgacg tggtggtctc ggccgcgacg
      661 ggtccgagct cggggtcggc gggcgagttg gccccctcgg tcacgcaggc ggccacgccg
      721 gcgacgaaga ccgccgtcac cagccagcgc agcaggcgcg ccccgcgcac gtcgtcgacc
      781 gtgggcagcc aggccggcgc ccacgacggc gggtcgagga gggggtcgcg cacccgctca
      841 tcgtgccgca cgcgcgagcc gttccgtggc cgctgccaga accctcggat cagctcccgt
      901 acaagccgag cgtctcggcg gcctcggcgg agaggtggta gctctcgtcg tcgttcggga
      961 acgctccgga gcggacgtcg gcggcgaagg ccgacagcgc gtcgaccgag gccgagccga
     1021 tctcggcgta gcggcgcacg aacttggggg ccatgcggtc ctcgatgccg agcacgtcgt
     1081 ggaacaccag cacctggccg tcgcagtccc ggcccgcgcc gatcccgatg gtgggcacgt
     1141 cgaccgcgtc ggtgaccatg cgggccacgg cgtcgggcac gccctcgagg acgatggcga
     1201 agcagccggc ggcggcgagc gccttggcgt cggacaccag ctccatggcg gcgtcgtggt
     1261 gcttgccctg caccttgaac ccgcccatgg cgtgcacgga ctggggggtg aggcccaggt
     1321 ggcccatgac cgggatctcg gcgtcgagga tggcctcgat cacggggagg cgcttgcgcc
     1381 cgccctcgag cttcaccgag tgggcgccgg cgcgcacgag ggcggcggcg ttgcggacgg
     1441 cctcgtgggt gtcggtgtgg tagctcatcc acggcaggtc ggcgacgacc atgggcaggc
     1501 ggccctcggc ggtggaggcc ttcagtcccc gggccacggc gccggtgtgg tgggcgatgt
     1561 cgtcgacggt gaccgagagg gtgtcgtcgt ggccgagcac gaccatggcc accgagtcac
     1621 cgacgaggat gaggtcgacg ccggcggcgt gggccatctt ggcgccgggg gcgtcgtagg
     1681 cggtcaccat gaccagcggc tgggccgacg tgccgtccgt cgtcttgacg gcgcggatcg
     1741 agggcgcgtt ccaggacgtg cgcatgtggt gctcctctgc tcccgtccag cggctgggtg
     1801 cctgccggcg gtgatccgag cgtacccccg tcgggcgggc ggagccaccc cctgtcggcg
     1861 ggatcaccgc gtgccggtag cgtcgccgcc atggccatcg ggacatgcga cagctgcggc
     1921 agcgaagagg acgtcaccga ggtgcgccgg gtgtacgtca ccccggagga ctgggacacc
     1981 gagggcaagg ccgaaccgca gccgggcacc gagatgtggt gctacgcctg ccgggcccac
     2041 tacccccacc aggaggtgtg agggcccccg ccctcagtcg ccgtcgatga tcgtgccgcc
     2101 cccgcccggg cgggtggtgg tcgtcgtcga ggtcggcgct gccgtggtgg tcggcgccgc
     2161 cgtggtcgtg gtggtcgacg gcggggccgt ggtcgtggag cggggctggg acgtggtggt
     2221 cgtgtcgtcc tcctcctcgt cctcctcgtc gtcgtccggt tcggtcgtgg tggggtccgg
     2281 gtcggtggtg gtcgtggcgc gggtggacgg tggcgcctcg gtggtggtgg tgaggtccgg
     2341 gttcaggggc tcgccggtga aggtcggctt cgggaagtcg ccgtaatcct cgccggccac
     2401 ggccgcctgc atgaaggtcc gccagatctg ggcggggaac gagccgccgg tgacgtcgcg
     2461 cccgcggacg ttgcgcatga agcggggctg gccgtcctcg tcgacgtcgg ggtaccccat
     2521 ccacaccgcg gtggtcagct gcggcgtgaa ccccacgaac caggcgtcgc ggtagtcctg
     2581 ggtcgtgccg gtcttgccgg cggcgggcac gccgatgctc gccccggtgc cggtgccgct
     2641 cagcacgttc tggcgcagcg tccaggcgac ctgcgcggcg gtggactccg agacggcccg
     2701 ctgggtccgg gctgcgctcg tcgacaggac cttgacctcg ccgtcgacga cctgctcgac
     2761 cttggtgatg aagatcggct cgatggactc gccctcccgg gcgaaggacg agtacgcgct
     2821 ggccatgtcg agcaccgaca cctcgccgga gccgagcacg agggagttga ccgccggcag
     2881 ctcgctgcgg acgccgagct ttcgggcgac gtcgacggcg ttgcccggcc cgacctcgat
     2941 catcaggtcg gcgtagaccg tgttggacga cacggtggtc gcctcgacca ggtcgatcac
     3001 gtcctcgttg ccgcgcgcgt agttggtgac ctcccagtcc tcgccggcgt tggcctcggg
     3061 gaacgtgcgc tcgtagacgt tgcggaactg cgagttcagc gagatgccct gctcgagggc
     3121 ggtggcgagc acgatcggct tgaacgacga gccgggctgg cgaccggacc cgccggcggc
     3181 ggcgccgagg gcgaggttga cctcggaggc gtcgaagtcc ttgccgccga ccatggccac
     3241 gacccggccg acgtcgtcga cggagacgag cgcgccggag gggtcatcgg gttcgttcag
     3301 cgtgtcccag acggcgtggt tcgcctcgcc ctgcagaccg aggtcgaggg cggtgtacac
     3361 ccgcaggccg ccgccgttca ccacgtcggc gccgtactcc tcgtagagca ggcggcggac
     3421 gtactcgacg tagtaggtgg ccccgacgtc ggcgcccttc accatcgaca cgccgtcgcg
     3481 cgccgctcgg ggttggacca tgtcgccgat cggcacggcc gcggcctcgt cccgctcggc
     3541 ctgggtgatg gcgccggtga cgatcatgcc ggccagcacc gaatcgcgcc ggaaggtggc
     3601 ccgctccggc tcggtggtgg catcggcgct ctcgggcgca cgcacgaggc cggcgaggta
     3661 ggcggcctgg gccaggtcga cgttgcggat cgagacaccg aagtacgcgc gagccgcggc
     3721 ctcggcgccg taggcacccc ggccgaagta cacggtgttg aggtagcgct ggaggatctc
     3781 ctgcttggac aggtcccgct cgagcttcac cgcgagcacg gcctccttga gcttgcgggt
     3841 gatcgtgcgc tcggaggaga ggaaggcgtt cttggcgtac tgctgggtga tggtcgagcc
     3901 gccctgctgg acgccgtcgc ccttgaagtc gcggtagacg gcgcgggcga tgccgagcgg
     3961 gtcgatgccg gagtgggaga agaactggcg gtcctcggcg gcgagcacgg cgtcgatgaa
     4021 gatctggggc atctcctcga gcgggaccga gacgcggtcc tcctcgccgg agagcttggc
     4081 catggcgttg tcggcgctgc agccgtcggt gacgtcggcg gcgcacacga acgtggactg
     4141 cagcagcgga tcggcatccg ggacgtcgac ctgggccagg acgaagccgg cgccggcgat
     4201 gctgcccacg aacaggagcg cgccgaggaa caggctgcgg cggaaccgcc agaggacgct
     4261 acggcgcttg ggcgccggct tggccttggg cttccgggac gggctcgggg tggcgggcgg
     4321 tgccatggga gaaacccggg aacgggcgag caccgatggt accgccggcc ggtcgccgtt
     4381 cccgccacgc gggggtgccg agcagcagga ccggcagggc caccgccacc accacggcga
     4441 ggctggtgcg gtactcggcg aggtcgacgc cggcccagcc ccaggtcggg acgaccgcga
     4501 aggcgacggc cacggcagcg agcagcccgg gccgggagcg gtcgtcgacc ggcgccagcg
     4561 gcccggtcga ggtgaccgcc acgacggcga tgatcgccgc gccgaccgcg gcgtgggcgg
     4621 gctggtcggc caggccgatc acgtcgatgg ccgccacggt gcccggaagg agggcgagga
     4681 gcgccagggg gtggcgaccg gccagcgcca gcaccgcccc cgccccgagg agcgcaccgg
     4741 cgctgcggag ctcgccgacg tcgccggcga ggagggcggc ggccaccgcg gtggcagcga
     4801 gcggcaggcg ctcggtcagg gcgcgggacc gcacggcgcg caggacgacg gcagcgacag
     4861 cgaccagcgc ggcgaccacg atgagggtcc agtcggtgcc gtccgcggcc tcgcgcagcg
     4921 gggcggcccg ggagacggcg agcaggccgg cgggcagcag gaggcgacct ggagcgcccc
     4981 acaccgcggc cgcgaccacg gcgacggtgg tgccggcgac ggcgagggcc accgcctcgg
     5041 cggggtcgtc gccgatgagg ccggtggcga tgacgacgcc ggcgagcagg gtgagcagct
     5101 gaccgagccg gggcgcgcgc tcggtccaca ggcgcgcggc ggcgagcacc gcgacgagga
     5161 ccacgccggc cgcgaggcgg cggtcgggca ggcggtcggg atcgatggcg gcggcggctg
     5221 ggagcagggt gaggatgagc agcgcggcac cggcggccgt ggccgagtgc gggcgggtcg
     5281 ccacgacgag cagtgcggtg ccggcggccg cggcggcgag cagcgggtcg gtgaccaggt
     5341 cgccccacgt gaccggggca tcctgggcgg cgaggaccac ccagccgaca gcgacgccgg
     5401 cggcgatgga acggcaggca cgggtggcgt ggtggggcag cacgaacgcg acggcggcgc
     5461 cgaccacggg tgcggcgagc agcaggagca ggagcgcggt cacgacggcc acccggcgag
     5521 gaccgcggcg gcgacgccgg cgaccagcgc ggcccacgga cgggcgcgac cgaacgccgt
     5581 ccgggcatcg gtgaacgtca cccccgcggc cagggcgacc cccaccaccg acgccagcac
     5641 gcgcacgccg agatcgctcg gaccgggccc gaccacgagg gcggcggcga acagtccgca
     5701 ggccagggcg ggggtggcgt cccgaagccg atccaggcgg ccggtggcgg cctgcagcga
     5761 gggccggacc agcggccggg cggcgaggat cacggcggct gcggccagcc aggcagatgc
     5821 agccgccgtc gcggggccga ccgtgccggc cggtccgagg acgctctgga tgccggcgag
     5881 gtcgtcgaag gtcgcggtgc ggaaccggat ggcggtggcg accacggcca gcagcgcggc
     5941 gccggcggat cggcggtccc gggcggccac gcccaccagc agggcggcca gcaccatcga
     6001 ccccgtggag gtgaccgcca gcgccgcggc ggccagcgtc gccgccgtcg cgaccgcgac
     6061 gctggtcggc tgcaggctgg tcggcgggcc cggcgccgcg agcccgggcg cggctgcgct
     6121 cacgtgcccc ggaagccctc gaccgggaag gtctgcagca ggtggttcca gccgcagccg
     6181 acgcacacct cgatgacgta gcaggtgcac tcggtggtgg tgcggcggac cttgtccatg
     6241 tcggcgcggt cctcgatggc gtagccggac ttgggcaggt ggggaccgaa cgcgtaggtg
     6301 acgagcacca ggaaccggtc ctcgcagatc gggcagatct gggtggtctt gcggctgatg
     6361 tgctcggcgg cgcgccgcag ctcggggtgg gcgtcgcaca cctcgtgccg ggcgaggcgg
     6421 ccccgctcgt actcggcgac gagcgcgttc cgggcgatgc ggtggtcgac gacgccggcc
     6481 gggcgggcga cgccgactcc cttcgacagg gctccggggt ggaacggcac gaccggcagg
     6541 gtagcgacgc ccgccgcgcc ctcgtcggcc gtgctcctag atgccttcct tgcgcgcccg
     6601 caggatcaag gtggcgggca ccttctccat cgccgggctc cagtgcgggc cgtggcccgc
     6661 cccggtgggc gccggctcca gcacgtggtc gacccggaag ccggcccggg tcagctcggt
     6721 gaacagggca ccggtcgagc gggcgtgggt gccgtgccgg ggatcgccgg gggcgtaggg
     6781 ctgctcgtcg aacgcagagc ggcggacctg gagggggtcg ggaccgtcgg ggtcgagcag
     6841 cgcgaaggtc gggtggggca cggagaccac gaagctgtgc tcgggacgca gcacccggtg
     6901 gatctggcgg aacacccggc ccacgtcggc gacctggacc agggagtgca ccgagatggc
     6961 ggcgtcgatg gtgtcggcgc gcaggaaggc cagctcggcc aggggcgcgt ggtgcagctc
     7021 caccttcatg tcggccgcgt cgatggcccg gcgagcggcg ccgatctgct cggcgtcctc
     7081 gtcgacggcg atgacccggg cgccggcgcg ggccatggcg atggcgttgc ggccggcccc
     7141 gcagccgagg tcgagcaccc gcttcccgga caggtcgccg agcagccgca gcaccggctc
     7201 gcccgggatc tcgggtccgt acaggacggc atcgtcgggg aggtcggcgc cgttgggcgc
     7261 agccacggtg aacggggtcc aggccatggt ccgagtctcc tacggacggg cggtcggggc
     7321 gaggatcctc gcgctacacc agcactgcca gatctaggag gagcgcttcg cccaccaggc
     7381 caggacgcgg cggcgggcct cgtcctcgcc gaggggacct tcttccatgc ggacctcgag
     7441 cagcatccgg agcgcctgcc ccacgaccgg tccgggtcgc acgtcgagga ggtccatcac
     7501 ctgctggccg tcgaggtcgg ggcggatggc gtcgatggcc tcctgctccc ggagtgcggc
     7561 gatgcgctcc tcgagcgcgt ccatgcgccg ggccagcatc tcggccttgc gccggttgcg
     7621 ggtggtgcag tcgcagcggg tgagctcgtt gagcttgtcg agcagcgggc cggcgtcgcg
     7681 cacgtagcgg cgcaccgcgg cgtcggtcca cgcgtcgtcg ccgtagccgt ggaagcgcag
     7741 gtgcaggaac accagctggg acacgtcgtc gcggatctcg ttggggtact tcagctcctt
     7801 catccgcttg cgcgtcatgc gggcaccgac cacctcgtgg tggtggaagg tcacgccctt
     7861 ggggccgaag tcgcgggtgt cgggcttgcc gatgtcgtgg aagagggcgg cgaggcgcag
     7921 caccaggtcg ggcgaggtct tgcggaccac ggcgatggtg tgcgccagca catccttgtg
     7981 gtggtggatg gggtcctgct cgaggcgcat ggcgctgagc tcgggcagga agtgctcgga
     8041 caggccggtg tcggccagga accacaggcc gggcgagggg tcgtcgagga ccaccaaccg
     8101 gttcagctcg tcccggatcc gctcggggga gacgatggcg atgcggtcgc ccatctgctc
     8161 gaccgcctcg accagctcgt cgtcgggctt caggtcgtga cgcgtcagga accgagcggc
     8221 gcgcagcatg cgcagggggt cgtcgctgaa cgactccgac gccgacagcg gggtgcgcag
     8281 caccttggcg gccaggtcgg ccgtgccgcc gaaggggtcg acgaggcggg cgtcgggtag
     8341 cgacagcgcc atggcgttga tggtgaagtc ccgacgggac aggtcggcct cgatgtcggt
     8401 ggcgaagctg acctcgggct tgcgggagtc gggcacgtag gcctcggccc ggtgggtggt
     8461 gatctcgtgg tcgacgacgc gggcgtcgtc gccctccccc acccgcacct tggcgccgat
     8521 ggtgccgaac cgcttgccct gggtccagag cgcctcggta ccggcgaagg acccgaggat
     8581 ctcctcgatg cggtcgggcg gggcgtcggt ggtgaagtcg tagtcgaccg ggcggtcgtc
     8641 accgagcagc aggtcccgca cgacgccacc caccaggtag aggcggtggc cggccgcgtc
     8701 gaacgcctgc gccaggggcg cgacgtcggc gagcacgggc gcgaatcgct cagggatcac
     8761 cggggcaacg gtaccggggg cgctcccggg cccaggattc gcttctgccg ctacatcagc
     8821 actgccagtt cgattcgacc ggaactggca gtggtggtgt tgcgctcaga agtggatcgc
     8881 gacctcttcc ttgaagcggt cgaggatgtc ggtgcgctgg ctgcccgtgc cgatggtgaa
     8941 gtcggggacg atgagctcgt cgacgccggc ctcgcggtag gcgccgacca cgtcgaccag
     9001 ctccgacggg gtgccgatga gcgtgggcat cggcatgtcc atgccacgca tccggtcgag
     9061 gaacgcctgg tcgtcggaca ggaacagcag cgcctgggtg gaccggtgga tctcggcggg
     9121 atcgcggccg aggtcctggc agtggccgtc gaggacgccg gacttgtggg cgaagatctc
     9181 tggggtgctc cagacgttcc actcctgggc gtgctcggcc gcgatgcgca gggtgcgcct
     9241 ctcgccaccg ccgccgacga ggagggggaa cggacccacc ggcttgggct ccatcggggc
     9301 gtcggtgagg tggtagtgct cgccgtggaa cgtggtgcgc tcctgggtgg acaggctgcg
     9361 cacgatggcg cacgcctcgg cgaagcggtc gaggcgctcc cccaccga
//
LOCUS       DATISU010000280        59933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_342711, whole genome shotgun sequence.
ACCESSION   DATISU010000280 DATISU010000000
VERSION     DATISU010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 59933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59933
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342711"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VLR27_10880"
     CDS             <1..577
                     /locus_tag="VLR27_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HSP03999.1"
                     /translation="QGALKALDAGVDGLVVEGGEGGGFKNPRDVASMVLLPLVAQHTD
                     VPIIAAGGMVDGPTMAAAFALGAEGVQMGTRMVSAAESPVHDNWKQAIVGAAETDTVF
                     LNRHSRPGLRALRTERTTELERQDSVDLGEAFGSVLDLYFGGDLEAGVALTGQVAGRI
                     DAVRPVAEIIDECVAGFHETIARLGGYGAAS"
     gene            574..1671
                     /gene="corA"
                     /locus_tag="VLR27_10885"
     CDS             574..1671
                     /gene="corA"
                     /locus_tag="VLR27_10885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HSP04000.1"
                     /translation="MTDTAAGPRSRLFHVDASGRRSELPDHRIDEIEGGWFWLDLQAP
                     TEARTHEIAERFELHRITREDLLEQQIPKFDDLDRYKVFVVHALAPDSDVLRTTEVDV
                     VVGQDWIITVHSGAVRSIDVLTERIHRDDFTLDGPFHLACRLFELVGERYLPILDDVD
                     TQVLDLEEGAVEGDPTVLPDIHALRRDLALLRRSLAPQRRMLELLTRSHGDSTDPAGR
                     DLADALDHHVHMIESLDSAHQMVATRVDTYRSATAERLNEVMKVLTVFSAIFLPMTLV
                     AGIYGMNFARMPELDESWGYPMALASMVVIAVGLWLYFVRRGFIGGPKLRKLIKPARA
                     VGRVGRGLASAAVQPVRAATRRNNGDGGAPD"
     gene            complement(1678..3231)
                     /locus_tag="VLR27_10890"
     CDS             complement(1678..3231)
                     /locus_tag="VLR27_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP04001.1"
                     /translation="MAIVHKSKFADVDIPSVPITEFVLRRADELADRQAIEEVGSGRG
                     YTFGALKQAIHALAGGLQAKGIGPGTTVAIMAPNLPEYAIVFHGVAVAGGTNTTINPT
                     YTADEVRHQLQDSEATLLVTIGMFVETAKAAIEGTSVTEIVTLDGAEGTTPLTSLFGD
                     PIEQVEIDPMEHVVVLPYSSGTTGLPKGVMLTHHNLVANIAQCEPVITYGDDEVAIAA
                     LPFFHIYGMQVLMNGLLANGVRVITMPRFDLQQALESIQEQKVTRFFAVPPMVLAFAK
                     HPLVAEYDLSSLRQVFSGAAPLGAELAEEAAARIGTEVVQGYGMSELSPVSHATPAGE
                     YKPGTSGVTIPNTECRIVDADGADQDVGGRGELWVRGPQVMKGYLNNDEATRSTIDED
                     GWLHTGDVAELDEDGHFSIVDRVKELIKFKGFQVPPAELEALLVSHPAIADAAVIGIP
                     DEEAGELPKAFVVVAPGQELSEDDVKEFVAGKVASYKQLRLVEFIDEIPKSASGKILR
                     RFLRDGTQD"
     gene            3291..4748
                     /locus_tag="VLR27_10895"
     CDS             3291..4748
                     /locus_tag="VLR27_10895"
                     /inference="COORDINATES: protein motif:HMM:NF005702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP04002.1"
                     /translation="MDVDLYDALVAGHDRDRPCLLLDDGGAWTYGDLARRSDELSGAL
                     RACGVDAGDRVVVQVDKSADAVALYLACLRSGAVHVPINTAYTPAEVAGLVDDARPTL
                     FLGRPGAPAPAGVRTETLGTDGSGTLPGHAAVGSAPVAPRSSDDWAAMLYTSGTTGRP
                     KGAMLSYGNLLSNARALVQAWRFEPDDVLVHVLPVFHVHGLFVALHCTLAVGATIRLH
                     ARFDADAVIADLPRSTVLMGVPTHYHRLLAHPDLDADRCVGMRLFTSGSAPLPAHEHE
                     AFARRTGHHIVERYGMTETMILTSNPYDGERIAGTVGHALEDIDLRVADDHGAPVPAG
                     TSGTVEVWGPNLFLGYWNRPEATAAAMRDDGFFITGDVGHLDDDGRLTLAGRASDLII
                     SGGYNVYPKEVELVLDQAPGVDESAVIGLPDPDLGEVVVAVVVGDGPVDEAAVLGACE
                     GLARFKRPRRLVVVDQLPRNAMGKVQKAVLRQQLG"
     gene            complement(4789..5208)
                     /locus_tag="VLR27_10900"
     CDS             complement(4789..5208)
                     /locus_tag="VLR27_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSP04003.1"
                     /translation="MSTQRNRTMSWAIPVVVVAVACVVAVVLVVSGGSSEEPPEPGSS
                     QELALGEEVYDQSCATCHGPGARGGLAGPPLVHEVYEDLTDAQIRTAIAEGKPPTNWP
                     QFEGGMAPVPGLSAGEVDAVIAYVRDVQRQEWGGDTP"
     gene            5311..5700
                     /locus_tag="VLR27_10905"
     CDS             5311..5700
                     /locus_tag="VLR27_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSP04004.1"
                     /translation="MPVDTKPTVLIVDDEVDIRLLVRAILESSDLGVDVVGEAVDGNE
                     AMEVFATLDPPEVPDVVILDNRMPGKEGLDVAAEMLEQEPRQHIVLFSAFVTPEIEER
                     AKALGVRACLGKNDFARLPELVIQLSR"
     gene            complement(5690..6058)
                     /locus_tag="VLR27_10910"
     CDS             complement(5690..6058)
                     /locus_tag="VLR27_10910"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HSP04005.1"
                     /translation="MNDAGPAPLLHLAVAAEWERARADGTYRRSTIDTSLEEEGFIHC
                     SFPEQVQATADRYYRGRDDIVLLRIDPDRLGVEVVVEEARSGERFPHVYGPIPVEAVT
                     SAEPVPMGPDGRLDLGWLSG"
     gene            complement(6055..7356)
                     /locus_tag="VLR27_10915"
     CDS             complement(6055..7356)
                     /locus_tag="VLR27_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP04006.1"
                     /translation="MGTPEEPAELFDLRMSEEAKPLLKRVTQFLEEVVAPMQEEYFAL
                     GANRENRWDYAPGQLELLDGAKAKAREAGLWNFFLPDSEVGGLSNLDYAYIAVELGKY
                     PLGSESLNCSAPDTGNMEVLERVGTPEQKKQWLEPLLNGEIRSAFAMTEPDVASSDAK
                     NVQLSAVKDGEEYVLNGEKYYISGAGDPRCKIMIVMCRTDPDAPPHKQQSQILVPMDT
                     PGVEILGPMHVFGHDDAPHGHMHLRFTDVRVPASNMLLGEGRGFEISQVRLGPGRIHH
                     CMRAIGQGEKALELLIRRAKFREAFGKPILSLGKNMETVSRARIELDACRLMVLKAAK
                     AMDVLGNQEARVYVSAVKAMVPEKVCQIIDQAIQVHGATGVSEWTPLAGMYTSQRTLR
                     LADGPDEVHHFVVARHETAQYSEPAPIGPSNDAEGIFAGPA"
     gene            7495..8265
                     /locus_tag="VLR27_10920"
     CDS             7495..8265
                     /locus_tag="VLR27_10920"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HSP04007.1"
                     /translation="MALTTDFRLDDRVAIVTGASSGIGHRFARVLAEQGATVLAAARR
                     ADRLEELAATSERIHAAACDVADDAQLEALVDRAVADHGRVDIVVNNAGTTDAVTPAE
                     HEDPALFRHVVDVNMNACFVLAAAAARQMIVQEQGGSVINIASVHGLVAAAPNAQAAY
                     AASKGGLVNLTRELAAQWAKHRIRVNAIAPGYFETELTEAMFVGEDAGLRYIRRNTLL
                     GRPGELAELDGPLLLLASDAGSYITGQTIAVDGGWTAR"
     gene            complement(8272..9354)
                     /locus_tag="VLR27_10925"
     CDS             complement(8272..9354)
                     /locus_tag="VLR27_10925"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HSP04008.1"
                     /translation="MPGQHLRRGRGAVLRALLAVVAVLVVGAALGAGGSLVFEERAGD
                     VRTADAPATAQLVPDAGPITIALVGDVAMGPAVAARLDEAPEDLVGPIAEVLRRADLA
                     IANLDVPIAVDPATVDVPAAPPGILGALVDSGIDVVSMANDRSLDLGPGGVYEMLAAD
                     EDLGALVGVGADEDAAYRPFVRDIGGHTVAVIGATQLLDAERIATDTAGPDQAGVASA
                     KRIDRLVAEVEAARAEADTVVVYVHWGAEGETCPGPGQQELATALVAAGADVVAGPGA
                     GRLQGAGRSGDAAVAYGLGRFIADDPSDPSTGVLLMQVDGSDVLDIEWVPAVRADGVP
                     VALEGDEATAAAGEWDSLRDCTGLTP"
     gene            complement(9400..10014)
                     /locus_tag="VLR27_10930"
     CDS             complement(9400..10014)
                     /locus_tag="VLR27_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04009.1"
                     /translation="MTTIIIGLLIVVLGTVAYIALRNAQDFSDANEVIPGVATSAPKE
                     WAGAHSVEARLHRRLRDSMTALRANRSLDEPAMIQVREALEREALAIDEQLVAAAALP
                     QRVRDEPLRQVATAVESVEATVADVVLLRGPERASTEAAIEQVRTRLALVAEARAELD
                     ALSGPSAVEQLRRELDLTAPAPPEQQPTTEPPPATEPPDEAPPA"
     gene            complement(10011..11189)
                     /locus_tag="VLR27_10935"
     CDS             complement(10011..11189)
                     /locus_tag="VLR27_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04010.1"
                     /translation="MVPPPPVGHAEEAAERLRERALLHRTEQTRTYPCLQCGGELEWH
                     IGKQRLVCPFCGNEQEVAVGDDDEVVEQDLHEAMAALDAGALTKTGLQVADEKEVVCQ
                     NCGGHTTFTGTLTTTRCPYCATPIQRDDVHDAPARLAVDGVLPFQVDRPTAESAIGEW
                     INSRWFAPNEFKKYATTGAFNSVYSAYFTYDALATTRYTGRRGDHYTVTVGSGENRRT
                     EVRTRWSYAAGTVQNAFDDVPVPANEGLDRKHVQALEPWPTADARPFSAEYLAGHLSR
                     TYDHDVVECFAPAKARMEDVIRETVRRDIGGDVQQITSMGVHYEGLTYKHLLLPVWLL
                     TVLYGGSPFQVFINGVTGEVQGERPYSKVKIALAVIAVTILALAAWFLFGSSGEGTAT
                     "
     gene            complement(11228..12379)
                     /locus_tag="VLR27_10940"
     CDS             complement(11228..12379)
                     /locus_tag="VLR27_10940"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HSP04011.1"
                     /translation="MGLLDKLKGELVDIIEWLDDSRSTLAWRFPRYQNEIKHGAQLIV
                     REGQRAVFVYRGQIADVFDPGNYELTSENLPILSTLQGWKHGFNSPFRSEVYFLNTRQ
                     VTDLRWGTPQPVTVRDPDFKMVQVRANGLCIVRIADAEVFLREVIGTDSAVEAEEITE
                     LLRRVITLAFSDMIMDTGLGAIDLQGRQVELSEKLREFVQDRVDDEFGLEITSVTMNI
                     SLPDEITQAMTRGVARGVEEGGYAHNVGDIGRLQQVRAADAMLAAAENEGGGVMGDAM
                     GMGMGIAMAGQMANQFGAATGQGQGQAGAAGPPPLPGQALFHVEVDGQATGPYSVQQL
                     QAGVAAGQVNAATLVWSNGMAEWKQASQVPALAPLFATPPPPPPPTTSE"
     gene            complement(12456..13955)
                     /locus_tag="VLR27_10945"
     CDS             complement(12456..13955)
                     /locus_tag="VLR27_10945"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HSP04012.1"
                     /translation="MDRRRFLLLSLATSGSLTIGRGALAPAFAAPAEPGASPFGPLLD
                     PDANGLRLPAGFTARVIKQFGEPIGPSTELAMPFPDGAATFPTADGGWILVSNSENPP
                     PVDAPDVTGTGITGLGGAQAIVFAPDGTIVDAYSILTGSRSNCAGGATPWGTWLSCEE
                     VDDPLDPASSGKVFECDPTGQRPAVDLPALGRFKHENATVDPERQQLYLSEDIGDGCF
                     YRFTPARYPDLSDGILEAASVGDDGRVTWLEVADPACERGVPCRQQAPATPFDGGEGC
                     VYDDCKVFLTTKGDDRVWMYDVVAETMVVLYDAGDFEEPVLTGVDHVIVQPGTGNIVV
                     AEDGGDMQAVMIRRDDYALFPILQMTGPQHGQQLPSPLDPVPIYSEVSGLAFNPAGDR
                     LYCNSQRGFVGGVTYEIQGPWLEALQRPADGAAGPPTSLPVRPDAGGGRPDGTPGVRP
                     AAAGTATTLPATGGTGVSAVGVGAAAGAAGLLALRRRLERRLPDGLPPA"
     gene            complement(13999..14439)
                     /locus_tag="VLR27_10950"
     CDS             complement(13999..14439)
                     /locus_tag="VLR27_10950"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP04013.1"
                     /translation="MPSHPQRSGQPTEPFSVRFDAHVRSVPESRRDFVAWMREADLDP
                     DTIDDLEVVFSELAANAVAASPEPTDDVHAQAQIDAGMLVLEVSNRTDDPQMLPQTPD
                     LDDPLRSNGRGLLIARAFVDSVEVEITGSNRLLVRCCRRLGLLR"
     gene            complement(14513..15682)
                     /locus_tag="VLR27_10955"
     CDS             complement(14513..15682)
                     /locus_tag="VLR27_10955"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSP04014.1"
                     /translation="MTYAGGRPILDADSHLMELPGFLDPYIEHDMLDRLKGRSMSRLA
                     PVLDKATGDAEARKADAQARAEAEERLLLDKGWNAMGAFDPAERSHVVDLFGFHGQLV
                     FATFASAMFVGKDLDLLYAGSAAHNRAVAAFCADDPRLLPVAYVPLHDPDRAVTLLQE
                     AIDLGSGAIHVPSTAAGERAPTHPDLDPFWARLEASGVPFVLHVGGGGRLLDRSFHNN
                     GRPVTDHLGGGENIRSKDFLAISNSPALFLGTLILDGLFDQFPGLRGGVIEEGASWVV
                     SWMHQLDFAQRAFRRTETPLQTLARTPSEYVHEHLRFTPFPGEPVGWMMEQAGADLFM
                     FSTDYPHPEGGRDPLAKFEATMADVTEADKHRFYYQNMADLLGPSLVPTKSGWID"
     gene            15783..16409
                     /locus_tag="VLR27_10960"
     CDS             15783..16409
                     /locus_tag="VLR27_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP04015.1"
                     /translation="MAADVAGTEPERLSADERRQALLDVTKALVNESGPDAVTMGTVA
                     ERAEVTRALVYKHFENKDALLLELYRREASALDRSIRHRVEAADEGFEAKLRAFVRSC
                     LDATGEHGQFFAPLRSARTSDDARRQQRRWDRRTSGYFAELAAEEFDIDPSTAATAIG
                     VLFSGIQNLLSQMRRSPSADRRDELEEVYVEMTIGALARLAAGRRPPA"
     gene            16475..17236
                     /locus_tag="VLR27_10965"
     CDS             16475..17236
                     /locus_tag="VLR27_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSP04016.1"
                     /translation="MAHEPGASLALAEAAEAGGWDGVFVSDAVMEDHDEPFVLLAAMA
                     ARTERITLGTWVVPLVARDVVAVARAAAGVDQLSDGRLVLGIGMGNEVEHRGLGVARD
                     GLGARHEAALDLLDRLLRGADVTVHDADHDLDGVALRTRPARSPRPPIMLAATWPNRA
                     GTRRAARWDGFMPQWPGLGEGADPDVAEGARIAELEALLGCYRDAGGDGPVAVPRTER
                     FGAEYDRRCEELGVDWLLTCDSLDEAALRAGPSAT"
     gene            complement(17254..17339)
                     /locus_tag="VLR27_10970"
     tRNA            complement(17254..17339)
                     /locus_tag="VLR27_10970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17303..17305),aa:Leu,seq:taa)
     gene            complement(17382..19892)
                     /locus_tag="VLR27_10975"
     CDS             complement(17382..19892)
                     /locus_tag="VLR27_10975"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HSP04017.1"
                     /translation="MTEDDGAPEPTRRDPSDPGDDPTVEQSAAEPGDHGGHEATGAEP
                     TSGLAAFRERARPAWRWLRWPVYGLAALFVLVLVAGAWLWTTTDLPPARSTSAAAVLL
                     DREGNELALLAQEGLRLEVPLEEMAPVAVDALIAAEDQRFYDHTGIDPVGIGRALWNN
                     LTDDSTQGGSTLTQQLVKNAYLNDDRTLSRKVREAVLAVKLERTEDKDQILERYLNTV
                     YFGRGSYGLEAAARTYFDSTAADLTAPQAAVLMGLLRSPTRLDPGEDLEASQERRDAV
                     LTAMVDMGTLTPEEGEAAIATPIEVTEAAASPTTLQEGVAPHFVEHVRGLLIEELGEQ
                     ALYDQGLVVHTTLDLADQRAAEAAVATHLDDPSDPQAAVVAVDRTGAVRAWVGGRDFE
                     TLQVDLAGGAGGSGRQPGSTFKPVTLAANFEAGNGAGEQYPAPAEITLDVAGGPWTVS
                     NAGGQDYGVITLADATVNSVNTVYAQALAKVGPQAVAELAQRLGIRRELQAEPSIALG
                     TEEVSPLEMASLYSTFARSGVSIDPFVITEVENRDGRLIWDPEGPTEEDAVEARIADT
                     VSAVLSETPRSGTARRAALDRPMAAKTGTTQRNADAWLAGYTPEYTAIVWVGNPDSNE
                     PIPDVDGQVVQGGTIPAMIWHDFMVEALADVPPTEFPEPDPELLSDTSPPMELTATPS
                     VEPGEAVDVIANGYQMCRAAWFVVLEGPTAVAEQETPEGQEPAPVEPVMVESPPDVDS
                     DEPTRRTSVQMPVTAVPGTYQVSARCDAGAGPQQLGPTAVVEVRPDPSATTTTSSTTT
                     STTTTTEPEEEPEPTTTTAPEQTTTTEAPPGQEDDEGG"
     gene            complement(19959..22211)
                     /locus_tag="VLR27_10980"
     CDS             complement(19959..22211)
                     /locus_tag="VLR27_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HSP04018.1"
                     /translation="MSSHHPDAPGPARPTGNPPGDDAPEAAPHRDGGGGLRGAWPWLR
                     WPVYGAAALLVLVLAVGAWLWTSTELPPTPSVAASAVLVDHTGEELAVLAQEGLRLEV
                     DLDEVAPVAVDALIAAEDRRFYDHGGVDPTGIARALWNNVTDDSTQGGSTLTQQLVKN
                     TYLTSDRTLTRKVREAVLAVKLDRTEDKDVILERYLNTVYFGRGAYGIEAAARTWFDT
                     TAADLTAEQAALLTGMLRAPESLGPDDHPDAARQRRDAVLDAMAAMGTLDPAEAERAQ
                     ATPVEVTPADPTPTVLRAGVAPHFVEHVRGLLVEQFGEQALYDQGLVVHTTLDIADQR
                     AAEAAVAAHLDDPDDPQAAVVSVDGFGAVRAWVGGRDFETLQVDLVSDAGGAGRQPGS
                     TFKPVTLAANLEAGNGAGQRFPAPAEMTFDIPGSEPWEVSNAGGSGYGTLSLADATVR
                     SVNTVYAQAVLQVGADAVVDMATRLGVSRELQPHPSIALGAQEVSVLEMATVYSTFAR
                     SGTRVDPFVMTKVETREGEVLWEQELEGEEAVDPAVANTVNAVLQESPRSGTARAAAI
                     DRPMAAKTGTTQNNADAWLAGYTPNYTTIVWVGHPDSNDTVEVDGRPVQGGTVPAAIW
                     HDFMVEAVQDVPAEDFAEPDDDLLRNPNRRSPAAEEAREEIDADQRATSSTTSTTSST
                     TTTAPEGEGEEEDEGDGDGDGEDDGDDGGGATTSTTSAPTTTAAPTTTAAPTTTEAST
                     TASGDAEGGG"
     gene            complement(22278..22601)
                     /locus_tag="VLR27_10985"
     CDS             complement(22278..22601)
                     /locus_tag="VLR27_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04019.1"
                     /translation="MQSYRIVLSHADIDGIPVDFDYADVFVVVREGATGPGPTDWEAQ
                     LRTDQYHRLAMARHDLALTAPDGSCMRGAAIVRFSDGHRHLFRGDDDLDGFVPHDPGG
                     DVVGG"
     gene            complement(22629..22931)
                     /locus_tag="VLR27_10990"
     CDS             complement(22629..22931)
                     /locus_tag="VLR27_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04020.1"
                     /translation="MSTSLRFAHAVRTLSESARLQGLEVPIFRTPPGRGDAVRTIRRN
                     RRGCTVAVRVGDRPWTAVLADLVDGIVLVNGLDGAAAIRCRTSLWTALEREAALAA"
     gene            23020..24000
                     /locus_tag="VLR27_10995"
     CDS             23020..24000
                     /locus_tag="VLR27_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HSP04021.1"
                     /translation="MPALATAWSPAQQRIIELLRRRPDDAPVDADVVAALHAELEERL
                     APVAERIPAGAALWVGKSALHQLDTCEGRWQAEASSRFAWNVPAARGTVAHKAIELQA
                     NWRGESEPCRLVDEAITRLAEGEGSLAAFLRALSTVELAELRQEAVGFVTAFEECFPP
                     LKSRWRPVVDGPSRVALAGGRIVLGGRPDLTLGRAADGGKVIIDLKTGRPSRHHVDDL
                     RFYALVDACKVGLPPRRLATLYLDTGTPTVEHVTPGVLQAATERVVRSVEAMVELRTA
                     ARPAQVRPNPMCRWCPALAGCETGSAWVAADADGEHEDGFWWPEPEVSQS"
     gene            24062..24910
                     /locus_tag="VLR27_11000"
     CDS             24062..24910
                     /locus_tag="VLR27_11000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HSP04022.1"
                     /translation="MTGEPIPTRVMEFDERFRDLPKHFAADGDIVMSHILAVLSSVFP
                     DGEDYFVRSVEAAVDDISDPELRHQVEGFIGQEEMHGREHRALNARLAELGYPTHAIG
                     VYVRGLTSLRERFQGRRANLAYTAALEHYTATLAETLLGDPDARAVVGHRGVRELLMW
                     HALEEAEHKSVAFDVYRHAGGTERMRVTLMWLTHLTFLLETSIWTTVSLAMDPAVRRR
                     PLRVLAGLWRLRRSPFARPRVVKQLFDYHRRGFHPTDRDTDALITEWRNTLFGRDGDL
                     TELVAS"
     gene            complement(25017..26303)
                     /locus_tag="VLR27_11005"
     CDS             complement(25017..26303)
                     /locus_tag="VLR27_11005"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP04023.1"
                     /translation="MAIDRPQAERARPGLREAGAALKIPDFRRFWIGATISNSGTWMQ
                     NVTVPYVLFQLTDSATWVGLASFLQFLPALFMGPLGGTLADRYPRRTVLLVAQSALAL
                     AAVGLWLLWSSGVAEPWSITAMVALFGAIAGTSIGAWQAFVSELVPRHLLLNAVTLNS
                     AQFNAARAVGPALGGLVLGTLGPSWAFGLNALSYVAVLVALATIHPAPVERAPRSGSV
                     LGDLRETAAYVRNMPGIATCIVVVIALGFFGNPVFPLLAVFAEQVFEVSGFRYGLLGA
                     ALGIGGVLATPLVAGRGSGLARSTLVGGALGVYGTSLLLFAVSPVFPMAVLFLVVAGG
                     GYLGLSSTLNTTIQVQVDEHRRGKVLALYVMGLTSSYPIGSLVQGALADVVSVRAVTA
                     FSGVVLLVVLARLLQTGRLRSMDDDPHAFDEAAEAG"
     gene            26406..27293
                     /locus_tag="VLR27_11010"
     CDS             26406..27293
                     /locus_tag="VLR27_11010"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HSP04024.1"
                     /translation="MARPTAEPVRTPGQLLPVHVPAPAAPELTDPRVSDVDEWGRSER
                     TRRAARALFAPMYDHWFRVEWEGLEKIPADGGALLVANHAAAIPSDAPVIMHGIETEL
                     QRAVYGLADELFKKVPVVGTLWSRAGGVLAHPDNAYRLLRQQQQLVLVFPEGSKGPGK
                     TFDERYQLRRFGRAGFVQIAMRAGVPVVPIAVVGAEESMPTLANLAPLAKALGLPYAP
                     VTANMLAFGPLLGPLGALVHLPAKFKLRVLDPVHFDVPPDQPRYSRSRVMDEAESIRQ
                     QIQDALYDMLRHRRSVWSG"
     gene            27293..28324
                     /locus_tag="VLR27_11015"
     CDS             27293..28324
                     /locus_tag="VLR27_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSP04025.1"
                     /translation="MGKRVLVTGLGTFWGARVAKALEADPDVDVIVGLDTREPLLELE
                     RTEYVKSDENYSILSRIVEATQVDTIVHTFLVVDSTQMRGRTMHEINVIGTMNLFAAA
                     TDSSVRTVVVKSSSYVYGCEPSDPVWFTEETPRQNPPKHRVERSLAEVESYVRDFAVD
                     NPHVAVTMLRFSNVLGPELSTPLSRMLELPLVPHVFGFDPRFQFVHEDDVVRAILFVL
                     EHELAGVFNVAGDGLLPWSEVAGICGKRTVPLPPIGQGLVTAALSRLGVDIPPEIVDL
                     LKYGRGLDNGRLKRAGFDYTHTSAGAVQAFIESLRLKRTVGPKAEYQYEREVEDFFRR
                     SPAVVRRPR"
     gene            complement(28321..28791)
                     /locus_tag="VLR27_11020"
     CDS             complement(28321..28791)
                     /locus_tag="VLR27_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP04026.1"
                     /translation="MEPVHVTELNHAVLYVRDVPVAVDFYRDVLGFQVVDEMPGRAAF
                     LRAGGSSNHHDLGLIGVGAGAPRPPHGATGLYHLAWRVGSIHELARAREVLASAGALR
                     GQSDHGVSKSLYAADPDGNEFELMYLLDEDEWGEYATRAVVQPLDLESEVARRS"
     gene            complement(28898..29467)
                     /locus_tag="VLR27_11025"
     CDS             complement(28898..29467)
                     /locus_tag="VLR27_11025"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HSP04027.1"
                     /translation="MTDTLTRTTADTTVPVAGTYQLDPSHSSADFQVRHLGLSKVRGG
                     FAIESGTVVVADDPTQSSVEVSLDAASFNTGAEDRDVHVKSADFLDVEQFPKVTFRST
                     AVRQSGDDWKVDGDLTIKDVSRPVTLDVEFEGANKDPWGNGRIAFAGETEINREDFGI
                     TWNQALETGGVLVGKNVKIMIDVQAVAEG"
     gene            29571..30056
                     /locus_tag="VLR27_11030"
     CDS             29571..30056
                     /locus_tag="VLR27_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HSP04028.1"
                     /translation="MALPTDPAEHLDDPRIEAYGMLIEAHNELHNALQRNLDAQAGVP
                     VGWLSVLIRLARSPDSRLRMTVLARDMTMSTSGLTRLIDRMEAEGVVQRQACPEDRRG
                     MLAVLTEQGRALLASAAPCHVADLEHLLGGALDQDELHELTDLLRRVRDHVRQVGPPP
                     G"
     gene            30085..30837
                     /locus_tag="VLR27_11035"
     CDS             30085..30837
                     /locus_tag="VLR27_11035"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HSP04029.1"
                     /translation="MSQVTTEITDGVAVVTLNDPDKRNALNLGMVEEVMAAMDAIEAD
                     ESVGAVVVTGEPPAFCAGADLSHLGSSQRDGLMSIYQGFLRFGRCPLPTIAAVNGAAV
                     GAGMNLALVCDVRIAAERAKFDTRFLQLGLHPGGGHTWMYRRIAGPQAVTAAVLLGQV
                     MDGKEAERVGLVHRCVPDAELLDHCIDMARASAEAPRDLVMKIKASIQDMADISDHDA
                     AVERELDPQVWSLNQPAFKERLAALQAKISKK"
     gene            complement(30865..31554)
                     /locus_tag="VLR27_11040"
     CDS             complement(30865..31554)
                     /locus_tag="VLR27_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04030.1"
                     /translation="MRVRAALLAAGLAAAALAGCGDDDDAPPPADPLPEQSELPGHGG
                     TEEADPGDLEVPEIAGTTFAPFPSLGFGIAVPQGWNATRLDDDALAELEDVRLAEPFF
                     LDAARNVASTGALFYAAGVDDEGRVAEIKVDVEEGASVEAVRTAAEAEVLAAGATDVN
                     VVEADDGRVRIDFRLAQPSAEDGETIDAYVSRLLVPSDDRLWSIVVTSEDAETQSAVL
                     AIVDAGFVIAD"
     gene            complement(31551..32162)
                     /locus_tag="VLR27_11045"
     CDS             complement(31551..32162)
                     /locus_tag="VLR27_11045"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HSP04031.1"
                     /translation="MSWSPAQTRLQGRRIVLRPLDVRDFPAWRDVRRRNTGWLTRWEP
                     RRIAGQPDVVESKDAFAARCSARAREWQLGTGYGFGVFSEDDALVGEINLSSVQRGPF
                     QNAYVGYWVDEAHAGRGYTPEAVVVACRYAFEELGLHRLQIAIIPRNRASRRVVEKLA
                     IREEGIALRYLEINGVWEDHVRYGITSEEWDTRRDELLEAWVS"
     gene            complement(32159..33244)
                     /locus_tag="VLR27_11050"
     CDS             complement(32159..33244)
                     /locus_tag="VLR27_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HSP04032.1"
                     /translation="MPRPDRILMGPGPGNPYPEAIEAFMRPMLGHLDPAFLELLDEVC
                     DQLRTVFGTNNALTIPISGTGSAGMETCIVNVLGPGDVAVIGVNGVFGARMCDVASRT
                     GAEVVRVDFDWGTPVTADRLLDAHPSPTLIGMVHAETSTGSRSDVEPVAAGKGDALLL
                     VDCVTSLGGIPFEADAWGIDLAYSGTQKCLGVPPGLSPITVSDRARERFVERSQSWYL
                     DLRMIGSYVGTAGAKRTYHHTAPISMIYSLHGGLTALLDEGIEAVHARHAECGQLLQD
                     GLVELGFELFATEGHRLPELTTVVVPDGVDEAAVRATLLERYDIEIGGGLGDYAGKVW
                     RIGCMGHTARPRNVTLLLGALREVLGR"
     gene            complement(33287..34660)
                     /locus_tag="VLR27_11055"
     CDS             complement(33287..34660)
                     /locus_tag="VLR27_11055"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HSP04033.1"
                     /translation="MADEADIAAVAELLGRAPQAHFDVVVRDDAGAPLVIRNAPLLFD
                     GTPMPTRYWLVGDEERARIGRLESTGAIDRAEAEIGEDVIAEAHDRYAAERDAAIPAD
                     HTGPRPSGGVGGTRRGIKCFHAHWAWHLAGGDDPVGRWIEAELAGQGGRVDAPVLAAI
                     DCGTNSTRLLVARGSGADKEPLARVNTITRLGRGVDATGKLDPEAIGRTIEVLRDYRR
                     IMDEHGADRVRVTATSAARDASNRDEFFDAAEEILGVRPELISGETEAELSFRGATAE
                     LDPADGPFLVVDIGGGSTEFIVGTEEVEGSMSIDIGCVRFLEQYVESDPPAPEELHAC
                     ISVTEAWMEDVARELPQLRSAKTFVGLAGTITTMAAVEIGLQEWDRDAIHHFRFQRDA
                     VEDVFRTLVTESMADRMHNPGLEPDRADVIVAGACIFVAIVRWLGVEECLVSESDILD
                     GLVDSLA"
     gene            complement(34667..35512)
                     /locus_tag="VLR27_11060"
     CDS             complement(34667..35512)
                     /locus_tag="VLR27_11060"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSP04034.1"
                     /translation="MELTNEFTVPVPIERAWEVLTDVELIAPCLPGAQLQEVEGDEYR
                     GIVKVKVGPITAQYKGAATFQEQDEAARKLVLKAEGRDTRGQGSASALITVNMSEQSG
                     QTHVTVDTDLTIKGKVAQFGRGMIADVSSKLMGEFVDCLEGKLDAPAAAATSAGTAAS
                     SASAASEVDSSATADAAESATGTEQAVGTPDGAEPTPVDGTAKVAADAASGTGATATP
                     VAGETGGAKAEPISGVRKIDSPDREPVDLVDAAGASLAKRVGPVVLVLLVLWLLGRRR
                     SNHGA"
     gene            complement(35503..36072)
                     /locus_tag="VLR27_11065"
     CDS             complement(35503..36072)
                     /locus_tag="VLR27_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HSP04035.1"
                     /translation="MVLAVLLAAGGGSRYEGDTHKLLAPLDGRPVLAHAIDALRASGL
                     EAVVVTGAADVAHLVPEGIATLSNPAWQAGQATSVRVGIEEARRRGHDAVVVGLGDQP
                     FVTAAAWQAVAAADAAVAVATYDGRRGHPVRLGDEVWDLLPVDGDEVGRAVMRGRPEL
                     VMEIPCDAGHTRTDRTADIDTLEDLRRWS"
     gene            36132..36518
                     /locus_tag="VLR27_11070"
     CDS             36132..36518
                     /locus_tag="VLR27_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04036.1"
                     /translation="MDPLAARRTLAQVRLAIGVGALVAPRLTGKLFGIDVAANPAAPY
                     LARLFGARELFMAAPFLMPAPGLDEAELASRAVPVDAADALASLAGGLRGYLPWRAAV
                     PATAAGLLGTWLGSQAAKNEKLIRPT"
     gene            complement(36650..37432)
                     /locus_tag="VLR27_11075"
     CDS             complement(36650..37432)
                     /locus_tag="VLR27_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HSP04037.1"
                     /translation="MIALFEVLRDAIAAEEPVALATVVEGPGVGGKLLVRPDREPIGS
                     LGDADLDRVVARDTLGELAAGTTGVRHYGTQGQANEHDVAVFVESFAPPPQLLVFGAV
                     DFTGALARVGKVLGYRVTVCDAREVFATKARFPFADEVVVDWPHRLLERVGDQLGPRD
                     AVCVLTHDPKFDVPAVIAALRTDVGYLGAMGSRRTTDDRNRRLRDEGVTDEQLARVMG
                     PIGLDLGARTPEETAVSIMAEVIATRTGRRAPSLRDAAGPIH"
     gene            complement(37429..37743)
                     /locus_tag="VLR27_11080"
     CDS             complement(37429..37743)
                     /locus_tag="VLR27_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HSP04038.1"
                     /translation="MREILGDLDRWRKAGKQVAVARVVDVEGSGPRLPGAAMAVNEDG
                     EVAGSVSGGCVEGAVVTEALQIIESGDRRIVTFGYSDDEAFAVGLTCGGTIHLFIEPL
                     DW"
     gene            complement(37754..38899)
                     /locus_tag="VLR27_11085"
     CDS             complement(37754..38899)
                     /locus_tag="VLR27_11085"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSP04039.1"
                     /translation="MVDIARDPATSAVAFVRALRQAGLVVPIDATATFAAALDHVDLG
                     TGDDVYWAGRSTLVRRADDVAAYDHLFAAFFGGVPLPRPAASSPQRITLAVDDPDAEM
                     DGGDDVADADPTVQVRFSRVETLRHKDFAAYDDDELAEAWALMDRLALVGAPRPSRRS
                     VPTRGVRGRPDLRRTVRHALRTGGEPIRRSHTQPGERFRRVVLLVDVSGSMEAYARPL
                     LRFVHAAVAARSRIEAFALGTRLTRLTRELRRRDPDEALRLAAAAVADFAGGTRLGEG
                     LGRFNDEWGVRGMARGAVVVILSDGWDRGDPEVLAEQMQRLQRVAHRVVWVNPLKATP
                     GYAPIARGMAAALPHVDAFVEGHSIDALERLAAVIAGEAAPVPAVAP"
     gene            complement(38908..39840)
                     /locus_tag="VLR27_11090"
     CDS             complement(38908..39840)
                     /locus_tag="VLR27_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSP04040.1"
                     /translation="MTPSAPPPADVRDARDVAAALEAHDYLPDTGLATAVFLALRLQR
                     PLLLEGDAGVGKTEVAKVLSRWTGGELLRLQCYEGLDVSQAVYEWDYSRQLLHLRAAE
                     AAGVAGGASVDQLEDELYDERFLVRRPLLEAVTPRSGPPPVLLIDEVDRADDEFEAFL
                     LEVLSDYTVTVPELGTFHADVPPLVVITSNRTRDVHDALKRRCLYHWVEHPDFEREVA
                     IVGVRVPEVGERLARQVAAATEALRGAGLYKPPGVAEAIDWAAALHVLGASELDVETV
                     DASLGTVLKYREDQARVRASGLDGLVARAVERSA"
     gene            39884..40180
                     /locus_tag="VLR27_11095"
     CDS             39884..40180
                     /locus_tag="VLR27_11095"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HSP04041.1"
                     /translation="MGLRHIVTLTFRDDTTDDQIHEIVEALRRLPSQIDEIRSYDVGT
                     DAELAERNAHLGIVADFDSVEGYEVYRDHPAHQAVITELIVPVIAGRSAVQHHI"
     gene            40189..41259
                     /locus_tag="VLR27_11100"
     CDS             40189..41259
                     /locus_tag="VLR27_11100"
                     /inference="COORDINATES: protein motif:HMM:NF014954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HSP04042.1"
                     /translation="MDDIAFISRPDQVSPEWLTAVLRAAGATDEGTVVDVKATPVGTG
                     QVGDSVRFALSWDDADAGPATVVGKFPTEDETSRATATAVRTYEVETRFYQQLRGRVD
                     IAAPVPFYAQIDMASHDFAILMNDLAPAEQGDQIAGCTPDEAAIAVEEAAKLHAPLWG
                     DSTLAGLDWMNRNSPEGLRGLVDMVWPGFLERYDGRLDAEVVDAGSVLVERLEAYLSW
                     RPEQLTAVHGDFRLDNMLFDPSPGGVPMTTVDWQTVSHGPGAQDVAYFLGTGLDPDLR
                     AEVEQDLVKEYLSTLRAGGVKDQSFEACWDQYRRSAFAGFIMAMISSMIVGQTDRGDE
                     MFMAMANRSGRMALDLDTLSML"
     gene            complement(41292..42530)
                     /gene="glp"
                     /locus_tag="VLR27_11105"
     CDS             complement(41292..42530)
                     /gene="glp"
                     /locus_tag="VLR27_11105"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HSP04043.1"
                     /translation="MIPLGAARRHVLDRVPRRAPVPVPVGDALGLVLGEDVVASEQVP
                     PFANTAMDGFALRAADTVGAPVELEILETIAAGHAPTAEVGPGQASRIFTGAPIPPGA
                     DAIVMVERTDLVDEGHVRVDIEVPVGNHVRPAGDDLDVGARVLAAGEVLSAAHLGVLC
                     SLGVESVLAHPRLTVGVVSTGDELVEGGQSLEPGQIRDSNRRTLLALAAGAGCDVVDL
                     GIAPDTEEAITDAIRRGVSECDAVLTSGGVSMGDIDLVKVILDRIGDMRWMQIAIKPA
                     KPFAFGLVDGVPVFGFPGNPVSSMVSFELLGRPALRQAMGHADLDRPIVRAVADAAFD
                     RRPDGKTHFARVVARHGADGRVHVDSAGGQGSHQLGAMAAANALAVLPDGDGVKAGGD
                     VDVLLLDDVPSESGLPIDPA"
     gene            complement(42548..43117)
                     /locus_tag="VLR27_11110"
     CDS             complement(42548..43117)
                     /locus_tag="VLR27_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04044.1"
                     /translation="MFTRCTKTLLTPVLALALLGAACGDDDDAAADAVGQDDSLDDAN
                     DAEETGDEATGDDEGAADEAEGEGTSGGDGTATLVLANGEEFEFSVLCSLEPQMAADQ
                     EILFSVTSYDEPYGFDATKFGEDAVMPGMASMDVYDSETYEAVWTASSVMGDVELELD
                     GSTVTGTGTFFEGDEGLGDGVAGELTASC"
     gene            complement(43159..44046)
                     /locus_tag="VLR27_11115"
     CDS             complement(43159..44046)
                     /locus_tag="VLR27_11115"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP04045.1"
                     /translation="MSLSLRARIVQRLIPKIMPGPELTPEEQRAKLSRSLGLPNRRVE
                     VVDLEVGGVPCLRVSPKGSPRRRHLLHVHGGGFVVGTTRGFAGYLSELALRADAVVTS
                     VDYRLAPEHPYPAAIDDCLAVYRVVADEVAPESLALIGESAGGNAVLGTMIRARDEGW
                     TPPNAAVLVSPWVDLTFSGDSMERNRSTELMLEPALFESWRDHYVGDRDPSDPGISPL
                     FAELWGLPPLHVQATAAEVLEDDARRLVERVREVGGDAELVVHDDLWHIFQTQAPLVP
                     EASDALDQAVTFIRTHTLG"
     gene            complement(44088..44798)
                     /locus_tag="VLR27_11120"
     CDS             complement(44088..44798)
                     /locus_tag="VLR27_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04046.1"
                     /translation="MSTVVVPEHFVGPPGMGHGGYVAGLLARHVDGAVQVTLRRATPV
                     GVDLDVDVEDDGRVVLHHDGELIAEAEAANLDLDVPPVPDIDAALAAEKGSPSHWNEL
                     GVHPICLGCGLARDDDDGLEIAVGPLEVDGHEQVAAVWRPTSAMAGPNGTVDPQWVLA
                     ALDCPGAMAFIARGTFAGLLGRIVFEQLAPAEVGIDHIVTGWEIDRDGRKLFAGTALA
                     TTDGTVLAQARATWFERT"
     gene            complement(44841..45716)
                     /gene="galU"
                     /locus_tag="VLR27_11125"
     CDS             complement(44841..45716)
                     /gene="galU"
                     /locus_tag="VLR27_11125"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HSP04047.1"
                     /translation="MTSVRTAVIPAAGLGTRFLPASKAIPKEMVPVVDKPTIQYIVEE
                     CVAAGIEDVLIIDSSGKSAIGDHFDRLMGLESALEAKGKTAELEMVRGLAELCDIHFV
                     RQGVALGLGHAIGIAERHVGDEPFAVLLGDDVQVDGGALLRGMIETHEEQQGSVIALL
                     EVEPEEISSYGCIDPIPIRDNLYGFERIVEKPSAEEAPSNLAVIGRYVFTPDIFDAIR
                     QTEPGVGGEIQITDAIGLLNKTQPVTGYSFREGRYDCGKPLDYLKATVEIACMRDDLG
                     PAFREYLKGFVATLD"
     gene            complement(45746..46357)
                     /gene="plsY"
                     /locus_tag="VLR27_11130"
     CDS             complement(45746..46357)
                     /gene="plsY"
                     /locus_tag="VLR27_11130"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HSP04048.1"
                     /translation="MSVLAVAVVVVGYVLGTFPTAVLVGRRHGFDPTTTGSGNPGASN
                     ATRVGGMRAGAVVLLGDAGKGMLAAGLGYVVDGRALAWAAGAAAVAGHLWPVTRRFRG
                     GKGVATAAGVALVCAPLVALPLAVVFAVLVKVTGTAAVGSIALAVLLPLGVLVRGQRW
                     YEVVVAAAIGLVVVVRHRTNIARLLDGTESVVRDRGRAPGGQA"
     gene            46428..46736
                     /locus_tag="VLR27_11135"
     CDS             46428..46736
                     /locus_tag="VLR27_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HSP04049.1"
                     /translation="MPTYEYRCKSCGHEMEVQQAFSDDPLDTCPECEGPLRKVYGNVG
                     IAFKGSGFYKNDARGSSKKSASSSSSTSDTSSSGSGSTEKKADTKTETKATPAASSSD
                     "
     gene            46849..47466
                     /locus_tag="VLR27_11140"
     CDS             46849..47466
                     /locus_tag="VLR27_11140"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HSP04050.1"
                     /translation="MRPAPARYPILRPPPLRLVLAVVLALVCGALVLRATDRAEAAVD
                     RYGSHTTAWVVASDLEPGDVIGSVDVDAESRPISFVPTGAVEADPTGRRATGALQRGE
                     VVVEHRLAGGDRRGAAALVPAGWRAVAIPALEAALPVEVGQRVDVLAALDPSGTGARG
                     SVVAEDGVVVHVADDDTVTVAVTPSQATRVVAALATGLVALALVG"
     gene            47506..49293
                     /locus_tag="VLR27_11145"
     CDS             47506..49293
                     /locus_tag="VLR27_11145"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSP04051.1"
                     /translation="MCSRWWRALLAVSFAFASVLAAPTPVAAQEVPAVTTHVVDSFDG
                     TPLLANLFVPSGVDGSRPAPLVLLTHGWGGTGQKAPTGFVADLLDAGYVVVTWDQRGF
                     GCSGGEVQIDKPEWEGRDVQALIDWAVANAPVQLDATGDPLVGMTGGSYAGGVQTAAA
                     SVDDRIDVLAPEISWADLRYSLYGGQVVNQGWATLLYLAGLASASGQGLDPSCPTHPQ
                     PGGLDPMITRGFTEGSTTAAISDEVLDFFAGSSLASYGQDQPVDIPTLVVQGSTDTLF
                     DLTDGWRIVEHVRATGAPTRFLVFCGGHVACPDTYADAGDGEHVDAAVLAWFARHLRG
                     DDTVDVGPPVEYRTNEGVWRATTGFPAPGSPPLEATGAGSLVSVPNVDAPDLSTLGGA
                     SFDGGIPALPFTSAAVSAPGDIRAMTVEVARATDRAVDLLGIPTVTLQVEGVGDEVIL
                     MAKLVDRESGAVVNLQEGAVRVPLLDGGAQVEVPMPGVAYTLPAGHHLDLQVSTASLM
                     HSNARTPATVDVIATVSVPVQAVDRAAAPAGPTSPAPAPTPAAPPVTLPATGAETSGT
                     VGAALAAAVAGVGLLLAGAAVRRRRGLSC"
     gene            complement(49283..50125)
                     /locus_tag="VLR27_11150"
     CDS             complement(49283..50125)
                     /locus_tag="VLR27_11150"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HSP04052.1"
                     /translation="MPLLHAIVLGIVQGLSEFIPISSSGHLAIVPWLFGWDDFAGDDS
                     LAKAFDVALHIGTLAGAIAYFWRDLIVFAREGLRVVVPANRADAPPEGRIAWLLVVSA
                     VPAAITGFLLNDVIEDLDDIFWLIGVMLIVGGVLLWAADRMGGPREAGEFRLRDALLM
                     GLGQAAALQPGVSRSGVTITVSRALGFDRAAAARLSFLMSLPIIAGAGLYKLLDVVGE
                     GGIPSDFVAPFLVGMATSAVTGFAAVWGTIRFVQTRTFAPFVGYRFVVGAGVIALAVA
                     GFNS"
     gene            complement(50199..51575)
                     /locus_tag="VLR27_11155"
     CDS             complement(50199..51575)
                     /locus_tag="VLR27_11155"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HSP04053.1"
                     /translation="MSAPAEPRTGTDVDALRALADRVASQAGPGEQVEAYVSQGTNTS
                     VDAYEGEIESLTQAGSAGIGIRVIKDGRTGFAYAGSHDDDIVADALAEARDNLPFAEP
                     DEWAGLAEPDGVAATSLDVHREAVLATPVEDKVAMAIELERMVRGADPRVRGVRAATY
                     TDAEGAFAVATSTGISVGGRSTIAYVSVSALADDGDVSVIAGGMSVGRAIDEVSLDEA
                     ADDAVSRLLAKIGSVKPESAVVTLILEPRLTATVLGIVSGMLGGEAVAKGRSPFANRI
                     GQQVGSPLLTIVDDPTNPDSLGAEPYDDEGLATRRVPLLQQGVLQGFLHNTHSARRAG
                     TTSTASAARSYSSRPGISARALTVEPGEGDLDSLLATVDDGVLASSLGGIHSGVNPVS
                     GDFSVSVEGLRVRNGERAEPIKEATLASTLQRLLLDVSHVGADVEWQPGGTGGVTLLI
                     PGVSLSGR"
     gene            complement(51572..52963)
                     /locus_tag="VLR27_11160"
     CDS             complement(51572..52963)
                     /locus_tag="VLR27_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HSP04054.1"
                     /translation="MIEDPTLERVLARALRTGGEFAEVFAEDKQSGNATLDDGRVEQL
                     SSGRDRGAGIRVVVGDTTGFAHTADLSEQGLLTAAEAASAAAREGGGGVREIALTRRD
                     VANPSPVSVLPDDVPKADKVAILRQADEAARAAAREISQVTASVAGSRRRILVANSDG
                     LLTSDDQVRTLFSVSAVATGDTGMQTGRKSTGLTIGWELFDRIDIDEFAAEAAQMAIT
                     KLRARPAPSGQLPVVIGAGSGGVLFHEACGHGLEKDLVDKGASVYAGRIGELVASPLV
                     TLVDDGTIAEEWGAFAVDDEGSPGQRNVLIQDGVLTEYMWDFLRSRKEGRANSGNGRR
                     QSYKHLPMVRMTNTFLENGTASPDDVIAGTDHGVYVAKLGGGQVNTASGDFVFGMQEA
                     YLIEDGEITEPIREGNLIGNGPQCLQDIEVIADDFAFGGPGTCGKDGQGVPVGDGCPT
                     LRVAQLTIGGTSS"
     gene            53041..53418
                     /locus_tag="VLR27_11165"
     CDS             53041..53418
                     /locus_tag="VLR27_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HSP04055.1"
                     /translation="MPLQTGLCATVEIVVTDDDTAIAIGSGSVPVLATPRLVALCEQA
                     AVAAIEGELTGGSTSVGMRVQVDHLMPTAVGAPVTAEATLEKVSGRRLTFTVSASDHR
                     GLIAAGRVTRVVVDYDRFMEKAN"
     gene            complement(53453..54283)
                     /locus_tag="VLR27_11170"
     CDS             complement(53453..54283)
                     /locus_tag="VLR27_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP04056.1"
                     /translation="MATRVIDISDGEHLTIDEVGVGGRGLLLVHGFTGGRIDFADHME
                     ALADAGWWVVAPDLRGHGDSWHPEDESEYSFDHFAGDMLSLVDALGWERFVLLGHSMG
                     GMIAQVAAAKRPDALDGLVLMDTTHGPLEIDRELAMLGAEIVREGGMVAVKEAMDAMG
                     GDGPLGTPANQRLLDERPGYRELGDQKFLGSSPAMYASMIGQMIDQLDRLQHLADLSV
                     PTLVIVGEQDAPFLDPSEAMATAVAGARLEVIADAGHSPQFENPDAWRAVMLDFLAGV
                     "
     gene            complement(54319..54993)
                     /locus_tag="VLR27_11175"
     CDS             complement(54319..54993)
                     /locus_tag="VLR27_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04057.1"
                     /translation="MPVRPRHLGCIALVAALVLSACSSSGASPSSPAPAAAVDPSSDP
                     AGSAPPAPEADGPHEVALVADFGPLPDGSGRAVVEATWTDDGDGTVGFVLDTPAGIVD
                     QHVLTADEHWWWLHPEARRTIADVEWIRFDLDVIAEAGGALPDVVLDARQPPPQPDEI
                     GPGDVVAGREVRAVDAVGEGEVRLTVAGVERPVVLRRRALPPGTTVDVPAGAVDVREL
                     PDHLQW"
     gene            55168..56079
                     /locus_tag="VLR27_11180"
     CDS             55168..56079
                     /locus_tag="VLR27_11180"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSP04058.1"
                     /translation="MTAGAVDQDDATTATDAQVAASRLRWWRELLYVAVFYAVYSSVR
                     NLFGSQGTGEDIDASVAFEHARQIIDIQRAMGLYFEAELQRWYLDLPAMGLIRVWNIF
                     YGTAHFVVTAGALVWLYRSDKARYPRWRNTLLVTTAVAIVGFASYSLMPPRLLDDPGK
                     YGACRIYAPEAAFTAEPGDKSVEGCDRYGFVDTLAVHGGWISFDDEKAAGLTNQYAAM
                     PSMHIGWSVWSALVLVPMMRRRWTRALAWSYPFLTLFTIMVTANHYWIDAVGGLVALG
                     FGALVARWLTARFQSRQARGDLPITTA"
     gene            complement(56132..57199)
                     /locus_tag="VLR27_11185"
     CDS             complement(56132..57199)
                     /locus_tag="VLR27_11185"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HSP04059.1"
                     /translation="MERSRDLPLIDVLALQRTACERAGSALYARILDAMADDVRTDGP
                     CTQILRPWAGNAMADAIPLRFLAAVHALVLTGDAPELAAHYASAGGSHTDDPVPAFLA
                     TVEAHRHRIEAAMDLGVQTNEVGRATSLFGGFHAVARRTGLPLRQLEVGASAGLLLHW
                     DRYGYRAGGGALAWGPDDGLVFEDPWTADLPPVATDLVVAERRGCDQAPIDPTTDEGA
                     TRLRSFLWPDQVERRARLDAAIEVARHHPVEVDAMDAGDWIEAQLAEPTPGLATVVHH
                     SIVLQYLPRSSFVRLRAAIEEAGARATPDAPIHWLRMEPAGDVADVRMSSWPGRAGGH
                     DEELLASTGYHGPPVTWLGGT"
     gene            complement(57216..58022)
                     /locus_tag="VLR27_11190"
     CDS             complement(57216..58022)
                     /locus_tag="VLR27_11190"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSP04060.1"
                     /translation="MPSLEGARARGESAIDSIRRRLTQQAADLLNEHPDFARGASEVG
                     LIDRAWLEQPTERPIRTATSLDVIQRFLERSIEQEPSLIANMGLNAIQLLNVDTDDGR
                     TGDARQVATIAFTDLEGFTSFTSREGDRAARELLELHHRAVGPVIRSRGGRIVKKLGD
                     GLLISFGAPEAAVLAALEINETNETGLRLRVGLHHGEVELLGDDVIGHDVNLAARVAD
                     SAKGGQVLATTDVRSAIGSPPPGVEFGRARRRAFKGVEGSVLVCPVRRAA"
     gene            58100..>59933
                     /gene="dxs"
                     /locus_tag="VLR27_11195"
     CDS             58100..>59933
                     /gene="dxs"
                     /locus_tag="VLR27_11195"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HSP04061.1"
                     /translation="MLLDRINSPADLRGLSADQLETLAAEIRTFVVDAVDRTGGHLGS
                     NLGAVELTLAVHRVFESPRDIVLWDTGHQAYVHKIVTGRRDGFANLRQEGGLSGYPSR
                     KESPHDWVENSHASTIISYAHGLATAMSRNEITDRRIVAVIGDGSLTGGMAYEGLNNL
                     GHAGSRAIIILNDNGRSYAPTVSRLGDSLARLRIDPRYVRNRARFEKLLRNVPRVGEM
                     LERGFDATLAGVREYFEPPAFFEDLGIRYTGPFDGHDIEGLESALHDAAEYDGPIVVH
                     VLTQKGRGYAPAENDPIKNMHDTGSVEPGSCAAAFTEALIKEAEQRPEVVAITAAMPD
                     STGLLPFAERFPDRLIDVGIAEQHAVTSAAGMAMGGLRPVFAVYSTFLSRAFDQVNLD
                     VGLHDQPVVFCLDRAGITGADGPSHHGVLDLVLFTKIPNMTVLAPSSYQEVGVMLHDA
                     LDQSDGPVLIRWPKVLPECGPDEVGSGLKGRKVRHGRDVCILAVGKMLHPAVEAAEAL
                     AADGIEATVWDVRCVKPADREMLDDAAGHPAVVTVEDGYKAGGAGSLLAAGVRECCEE
                     RLVPRIEILGVPTEYLDHAKVDAIMGRLCLDADGIRSSVLRAVRA"
BASE COUNT         8083 a        21480 c        22040 g         8330 t
ORIGIN      
        1 gcagggtgcg ctcaaggcgc tcgacgccgg cgtcgacggc ctggtcgtgg agggcggcga
       61 gggtggcggg ttcaagaacc cgcgcgacgt ggcctcgatg gtcctgctgc cgctcgtggc
      121 ccagcacacc gacgtgccga tcatcgccgc cggcgggatg gtcgacggcc cgacgatggc
      181 ggcggccttc gccctcggcg ccgagggcgt gcagatgggc acccgcatgg tctccgcggc
      241 cgagtcgccg gtgcacgaca actggaagca ggccatcgtc ggcgccgccg agaccgacac
      301 cgtgttcctc aaccggcaca gccgccccgg cctccgcgcc ctgcggaccg agcgcaccac
      361 cgagctggag cgccaggact cggtggacct cggcgaggcg ttcggctccg tgctcgacct
      421 ctacttcggc ggggacctgg aggccggggt cgccctcacc ggtcaggtgg ccgggcgcat
      481 cgacgcggtc cgaccggtgg ccgagatcat cgacgagtgc gtcgccgggt tccacgagac
      541 catcgcccgg ctgggtggat acggagccgc ctcgtgaccg acaccgctgc cggcccgcgg
      601 agccggctgt tccacgtcga cgcgagcggg cgtcgatcgg agctgcccga ccaccgcatc
      661 gacgagatcg agggcggctg gttctggctc gacctgcagg cgcccaccga ggcccgcacc
      721 cacgagatcg ccgagcgctt cgagctgcac cgcatcaccc gggaggacct gctcgagcag
      781 cagatcccga agttcgacga cctcgaccgc tacaaggtct tcgtggtcca cgcgctggcg
      841 ccggactccg acgtgctgcg gaccacggag gtcgacgtgg tggtcggcca ggactggatc
      901 atcacggtcc actccggcgc ggtccgcagc atcgacgtgc tcaccgagcg gatccaccgc
      961 gacgacttca ccctcgacgg tccgttccac ctggcctgcc ggctgttcga gctggtcggc
     1021 gagcggtacc tgccgatcct cgacgacgtc gacacccagg tgctcgacct cgaggagggc
     1081 gcggtggagg gcgaccccac ggtcctgccc gacatccacg ccctgagacg cgacctcgcc
     1141 ctgctgcggc gcagcctggc accgcagcga cgcatgctcg agctgctgac ccggtcccac
     1201 ggcgacagca ccgacccagc cggacgcgac ctcgccgacg ccctcgacca ccacgtccac
     1261 atgatcgagt cgctcgacag cgcccaccag atggttgcga cgcgggtcga cacctaccgc
     1321 agcgccacgg ccgagcggct gaacgaggtc atgaaggtgc tcaccgtctt ctcggccatc
     1381 ttcctgccga tgaccctggt ggccggcatc tacggcatga acttcgctcg catgcccgag
     1441 ctcgacgagt cgtggggcta cccgatggcg ttggcttcga tggtggtcat cgcggtgggg
     1501 ctctggctgt acttcgtgcg ccgcggcttc atcggcggcc cgaagctccg caagctgatc
     1561 aagccggcgc gtgccgtggg gcgggtgggt cgaggcctcg catcggctgc ggtgcagccg
     1621 gtgcgcgccg ccacccgtcg gaacaacggt gacggcggcg cgcccgactg agcgtggtca
     1681 gtcctgcgtt ccgtcgcgca ggaagcggcg caggatcttg cccgaggccg acttggggat
     1741 ctcgtcgatg aactcgacca ggcggagctg cttgtaggac gcgaccttgc cggcgacgaa
     1801 ctccttgacg tcgtcctcgg acagctcctg gccgggggcg accaccacga acgccttggg
     1861 cagctcgccg gcctcctcgt cggggatgcc gatgacggcc gcgtcggcga tggccgggtg
     1921 gctcacgagg agcgcttcca gctcggccgg cgggacctgg aagcccttga acttgatgag
     1981 ctccttcacc cggtcgacga tcgagaagtg cccgtcctcg tcgagctcgg cgacgtcgcc
     2041 ggtgtggagc cagccgtcct cgtcgatggt gctgcgggtg gcctcgtcgt tgttcaggta
     2101 gcccttcatg acctgcggac ccctgaccca cagctcgccc cggccgccga cgtcctggtc
     2161 cgcgccgtcg gcgtcgacga tgcggcactc ggtgttgggg atggtgaccc cggaggtgcc
     2221 gggcttgtac tcgccggcgg gcgtggcgtg ggacaccggg gacagctcgc tcatgccgta
     2281 gccctggacg acctcggtgc cgatgcgggc cgcagcctcc tcggcgagct cggccccgag
     2341 cggggcggcg ccggagaaga cctggcgcag ggaggagagg tcgtactcgg cgaccagcgg
     2401 gtgcttggcg aaggccagca ccatcggcgg gacggcgaag aagcgggtga ccttctgctc
     2461 ctggatcgac tcgagggcct gctggaggtc gaagcggggc atggtgatga cgcgcacgcc
     2521 gttggcgagc aggccgttca tcagcacctg catgccgtag atgtggaaga acggcagggc
     2581 ggcgatggcc acctcgtcgt cgccgtaggt gatgaccggc tcgcactggg cgatgttggc
     2641 caccaggttg tggtgggtga gcatgacgcc cttgggcagg ccggtggtgc ccgaggagta
     2701 gggcagcacg acgacgtgct ccatcgggtc gatctcgacc tgctcgatgg ggtcaccgaa
     2761 cagcgacgtg agcggggtgg tgccctcggc gccgtccagg gtcacgatct cggtgaccga
     2821 cgtgccctcg atggcggcct tcgcggtctc gacgaacatc ccgatggtga cgagcagcgt
     2881 cgcctcggag tcctggagct ggtggcgcac ctcgtcggcg gtgtaggtcg ggttgatcgt
     2941 ggtgttggtg ccgccggcga cggccacgcc gtggaagacg atggcgtact cgggcaggtt
     3001 gggcgccatg atcgccacgg tcgtgcccgg gccgatgccc ttggcctgga gcccgccggc
     3061 cagcgcgtgg atggcctgct tcagcgcccc gaaggtgtag ccgcggccgc tgccgacctc
     3121 ctcgatcgcc tggcggtcgg ccagctcgtc ggcgcgccgc aggacgaact cggtgatggg
     3181 caccgacggg atgtcgacgt cggcgaactt gctcttgtgg acgatggcca tggcgctgac
     3241 tctatggggc aggtcggggc ggccccacgg cgggtgcgag gatgcgggcg atggatgtcg
     3301 acctctacga cgccctggtg gccggccacg accgcgaccg tccctgcctg ctcctggacg
     3361 acggtggcgc ctggacctac ggcgatctgg cgcggcgcag cgacgagctg tcgggtgccc
     3421 tgcgggcctg cggggtggac gccggcgatc gggtggtcgt gcaggtggac aagtccgccg
     3481 acgccgtggc cctgtacctg gcctgcctgc ggtcgggggc ggtgcacgtg ccgatcaaca
     3541 ccgcgtacac cccggcggag gtggccgggc tcgtggacga cgctcgaccc accctgttcc
     3601 tcggacggcc cggcgcgcct gcgcccgcgg gggtgcgcac cgagacgctc ggcaccgacg
     3661 gcagcggcac gctccccggc cacgccgccg tcggatcggc gccggtcgcg ccccggtcga
     3721 gcgacgactg ggcggcgatg ctctacacct cgggcacgac gggtcgtccg aagggggcga
     3781 tgctctcgta cgggaacctg ctgtccaacg cccgggccct ggtgcaggcg tggcgcttcg
     3841 aacccgacga cgtgctggtc cacgtgctgc ccgtcttcca cgtgcacggc ctcttcgtcg
     3901 ccctgcactg caccctcgcc gtcggcgcga ccatccggct gcacgcccgc ttcgacgccg
     3961 acgccgtgat cgccgacctg ccgcgcagca cggtgctcat gggtgtgccg acccactacc
     4021 accgcctcct cgcccacccc gacctggacg ccgaccgctg cgtgggcatg cgcctgttca
     4081 cctcgggctc cgctcccctg ccggcccacg agcacgaggc gttcgcccgc cgcaccggcc
     4141 accacatcgt cgagcgctac ggcatgaccg agaccatgat cctgacctcg aacccctacg
     4201 acggcgagcg catcgccggc acggtcggcc acgccctcga ggacatcgac ctgcgggtcg
     4261 cggacgacca cggcgccccg gtgccggcgg ggacgtcggg gaccgtcgag gtctggggcc
     4321 cgaacctgtt cctcggctac tggaaccggc cggaggcgac cgcggcggcg atgcgcgacg
     4381 acgggttctt catcaccggc gacgtcggcc acctcgacga cgacggccgg ctgaccctgg
     4441 ccggccgggc gagcgacctc atcatctcgg gcgggtacaa cgtgtacccg aaggaggtcg
     4501 agctggtcct ggaccaggca cccggggtgg acgagtccgc cgtcatcggg ctgcccgacc
     4561 cggacctcgg cgaggtcgtg gtcgccgtgg tcgtgggcga cggaccggtg gacgaggctg
     4621 cggtgctcgg ggcctgcgag ggcctggccc gcttcaagcg cccccgccgt ctcgtcgttg
     4681 tcgaccagct gccccgcaac gcgatgggca aggtgcagaa ggccgtgctc cgccagcagc
     4741 tgggctgacg ggcgcaacac cagcactgcc agttcgagga gggggcggtc agggggtgtc
     4801 gccgccccac tcctggcgct gcacgtcgcg cacgtaggcg atcacggcgt cgacctcgcc
     4861 ggcggacagg ccgggcaccg gcgccatccc gccctcgaac tggggccagt tggtcggcgg
     4921 cttgccctcg gcgatcgccg tgcggatctg ggcgtcggtc aggtcctcgt agacctcgtg
     4981 gacgagcggc ggaccggcca ggccgccccg ggctccgggg ccgtggcacg tggcgcagct
     5041 ctggtcgtac acctcctcgc cgagggcgag ctcctgggag gagcccggtt cggggggttc
     5101 ctcggatgac ccgccgctga ccaccagcac cacggccacc acgcaggcga cggccacgac
     5161 gacgaccggg atcgcccatg acatggtgcg gttgcgctgg gtgctcatcg gttcctgccc
     5221 atttcccgat cctgacacgg ccatgtgcga tgccgtgacc taccgtgtcg gcgcaggtca
     5281 cgcgggtggc tgctggggaa ggagcgtgac atgccggtgg acaccaagcc gacggtgctg
     5341 atcgtcgacg acgaggtcga catccggttg ctggtgcgag ccatcctcga gtcgtccgac
     5401 ctcggcgtcg acgtggtcgg cgaggcggtc gacggcaacg aggcgatgga ggtgttcgcc
     5461 accctcgatc cgcccgaggt gcccgacgtg gtgatcctcg acaaccggat gcctggcaag
     5521 gagggcctcg acgtcgccgc tgagatgctc gagcaggagc cccgccagca catcgtgctg
     5581 ttcagcgcgt tcgtcacccc cgagatcgag gagcgggcca aggccctcgg cgtccgggcc
     5641 tgcctgggca agaacgactt cgcccgcctc cccgagctcg tgatccagct cagccgctga
     5701 gccaaccgag gtcgagtcgc ccgtcgggcc ccatcggcac cggctcggcc gacgtcaccg
     5761 cctcgacggg gatcggcccg tagacgtgcg ggaagcgctc gccgctgcgg gcttcctcca
     5821 ccacgacctc gacgccgagg cggtccgggt cgatgcgcag cagcacgatg tcgtcccgac
     5881 cccggtagta ccggtcggcc gtggcctgca cctgctccgg gaacgagcag tggatgaagc
     5941 cctcctcctc gagggaggtg tcgatcgtcg accggcggta ggtgccgtcg gcccgcgccc
     6001 gctcccactc ggcggcgacc gccaggtgga ggagcggggc gggtccggcg tcgttcatgc
     6061 ggggccggcg aagatgccct cggcgtcgtt gctcgggccg atgggggccg gttcgctgta
     6121 ctgggcggtc tcgtggcggg ccaccacgaa gtggtgcacc tcgtccggcc cgtccgccag
     6181 ccggagcgtg cgctgggagg tgtacatgcc ggcgagcggg gtccactcgg acacgccggt
     6241 ggcgccgtgg acctggatgg cctggtcgat gatctggcag accttctcgg ggaccatggc
     6301 cttcaccgcg ctcacgtaga cgcgggcctc ctggttgccg agcacgtcca tggccttggc
     6361 ggccttgagc accatgaggc ggcaggcgtc cagctcgatc cgcgcccggg agaccgtctc
     6421 catgttcttg ccgaggctca ggatcggctt gccgaacgcc tcgcggaact tcgcccggcg
     6481 gatcagcagc tcgagggcct tctcgccctg gccgatggcg cgcatgcagt ggtggatgcg
     6541 ccccgggccg aggcggacct ggctgatctc gaagccacgg ccctcgccga gcagcatgtt
     6601 cgatgccggc acgcgcacgt cggtgaagcg gaggtgcatg tggccgtgcg gggcgtcgtc
     6661 gtggccgaag acgtgcatcg ggccgaggat ctcgacgccg ggggtgtcca tcgggaccag
     6721 gatctgggac tgctgcttgt gcggcggggc gtccgggtcg gtccggcaca tgacgatcat
     6781 gatcttgcag cgcgggtcgc cggcgccgga gatgtagtac ttctcgccgt tgaggacgta
     6841 ctcctcgccg tccttcaccg ccgacagctg cacgttcttc gcatccgagg aggccacgtc
     6901 gggctcggtc atggcgaacg cggagcggat ctcgccgttc aggaggggct cgagccactg
     6961 cttcttctgc tcgggcgtgc cgacccgctc gaggacctcc atgttgccgg tgtcaggggc
     7021 cgagcagttc aggctctcgg agccgagcgg gtacttgccg agctcgaccg cgatgtaggc
     7081 gtagtcgagg ttggacaggc cgccgacctc ggagtcgggc aggaagaagt tccacagacc
     7141 ggcctcgcgg gccttggcct tggcgccgtc gagcagctcg agctggccgg gggcgtagtc
     7201 ccagcggttc tcgcggttcg cgccgagggc gaagtactcc tcctgcatcg gagccacgac
     7261 ctcttcgagg aactgcgtga cccgcttcag cagcggcttg gcctcctcgc tcatccgcag
     7321 gtcgaacagc tcggctggtt cttcgggggt gcccatgggt ggtgcgtcct ctcgtacgtg
     7381 gtcggccccg tccctggagg cctgccgtcg aatgtaggcc caacgaggcc gcatccacgc
     7441 tccgccgcgg ttccgacccg tccgtccgga ggccacttcg gtagaaaccc tgccatggcc
     7501 ctcaccaccg acttccgcct cgacgaccgg gtcgccatcg tgaccggcgc ctcgagcggc
     7561 atcggccatc gcttcgcgcg ggtgctcgcc gagcagggtg ccaccgtcct ggccgccgcc
     7621 cggcgggccg accgcctcga ggagctcgcc gccaccagcg agcggatcca cgccgccgcc
     7681 tgcgacgtcg ccgacgacgc ccagctcgag gccctcgtcg accgcgccgt cgccgaccac
     7741 ggtcgcgtcg acatcgtcgt gaacaacgcc ggcaccaccg acgccgtgac cccggccgag
     7801 cacgaggacc cggcgctgtt ccgccacgtg gtcgacgtga acatgaacgc ctgcttcgtg
     7861 ctggccgccg ccgcggctcg gcagatgatc gtgcaggagc agggcggatc ggtcatcaac
     7921 atcgcgtccg tgcacggtct cgtcgcggcc gcgcccaacg cccaggctgc ctacgcggcg
     7981 tccaagggcg gcctggtcaa cctgacccgc gagctcgccg cgcagtgggc caagcaccgg
     8041 atccgggtga acgccatcgc gccgggctac ttcgagaccg agctcaccga ggccatgttc
     8101 gtcggcgagg acgccggtct gcgctacatc cggcgcaaca cgctgctcgg ccgacccggc
     8161 gagctcgccg agctcgacgg ccccctgctg ctcctcgcct ccgacgccgg ttcctacatc
     8221 acgggccaga ccatcgccgt cgacgggggc tggaccgccc gctgagcgcc ctcagggggt
     8281 gaggccggtg cagtcccgga gcgagtccca ctcgccggct gcggccgtcg cctcgtcccc
     8341 ctccagggcc accgggacac cgtcggcccg caccgccggc acccactcga tgtccagcac
     8401 gtcgctcccg tcgacctgca tcagcaggac accggtgctg gggtcggacg gatcgtcggc
     8461 gatgaatcgg cccagcccgt aggcgacggc tgcgtccccg gagcgtccgg cgccctgaag
     8521 gcgtccggcg ccggggccgg cgaccacatc ggcgcctgcg gccaccagcg cggtcgcaag
     8581 ctcctgctgt cccggtccgg ggcaggtctc cccctcggct ccccagtgca cgtagaccac
     8641 gacggtgtcg gcctctgccc gggccgcctc gacctcggcc accaggcggt cgatccgctt
     8701 ggcggacgcc acgccggcct ggtcgggacc ggcggtgtcg gtggcgatgc gctccgcatc
     8761 gagcagctgc gtggcgccga tgaccgcgac cgtgtgacca ccgatgtcgc gcacgaaggg
     8821 gcggtaggcc gcgtcctcgt cggcgccgac gccgaccagg gccccgaggt cctcgtcggc
     8881 ggcgagcatc tcgtagacgc cccccggccc caggtccagg ctgcggtcgt tggccatgga
     8941 caccacgtcg atgccggagt cgacgagggc gccgaggatg ccgggtgggg cagcggggac
     9001 gtccacggtc gcggggtcga cggcgatcgg gacgtcgagg ttggcgatgg cgaggtcggc
     9061 acggcgcagc acctcggcga tcgggccgac gaggtcctcg ggggcctcgt cgagccgggc
     9121 ggccacggcc ggccccatgg cgacgtcacc gaccagcgcg atggtgatcg gaccggcgtc
     9181 gggcaccagc tgggcggtcg cgggggcgtc ggcggtgcgg acgtcgccgg cccgctcctc
     9241 gaacaccagc gagccgcctg ctcccagggc cgcgccgacg accagcaccg cgaccacggc
     9301 gaggagggcc cgcaggacgg cgccgcggcc tcgtcggagg tgttgcccgg gcatcggggc
     9361 gacgctaccg acggcccgtg ccgccgccgc gccacccggt caggccggag gcgcctcgtc
     9421 cggaggctcg gtcgcgggcg gtggctcggt ggtgggctgc tgctcgggcg gtgccggggc
     9481 ggtcaggtcc agctcacggc gcagctgctc gacggcggag ggaccgctca gcgcgtcgag
     9541 ctcggcgcgg gcttcggcga cgagggcgag gcgggtccgg acctgctcga tggcggcctc
     9601 ggtgctggcc cgctccggcc cgcgcagcag caccacgtcg gcgacggtgg cctcgacgga
     9661 ctccaccgcc gtggcgacct ggcgcagcgg ttcgtcacgg acccgctggg gcagggctgc
     9721 ggcggcgacc agctgctcgt cgatggcgag ggcctcgcgc tcgagcgcct cccgcacctg
     9781 gatcatggcc ggctcgtcga ggctccggtt ggcccgcagc gcggtcatcg agtcccggag
     9841 gcggcggtgc aggcgggcct cgacggagtg cgcaccggcc cactccttcg gagcgctcgt
     9901 cgccacgccc gggatgacct cgttggcgtc ggagaagtcc tgggcgttgc gcagggcgat
     9961 gtaggccacc gtgccgagga cgacgatgag caggccgatg atgatggtgg tcatgtggcg
    10021 gttccctccc cgctcgagcc gaagaggaac caggctgcca gggccaggat ggtgacggcg
    10081 atgacggcga gcgcgatctt gaccttgctg taggggcgct ccccctgcac ctcgccggtg
    10141 acgccgttga tgaacacctg gaacgggctc cccccgtaga ggaccgtgag cagccacacg
    10201 ggcagcagca ggtgcttgta ggtgagcccc tcgtagtgga cgcccatcga ggtgatctgc
    10261 tggacgtcgc cgccgatgtc gcgccgcacc gtctcgcgga tgacgtcctc catgcgggcc
    10321 ttggccggag cgaagcactc gacgacgtcg tggtcgtagg tgcggctcag gtgaccggcg
    10381 aggtactcgg cgctgaacgg ccgggcgtcg gcggtgggcc acggctcgag ggcctggacg
    10441 tgcttgcggt cgaggccctc gttggcgggg acggggacgt cgtcgaacgc gttctggacc
    10501 gtgccggccg cgtagctcca gcgagtgcgc acctccgtgc gccggttctc ccccgacccg
    10561 accgtgaccg tgtagtgatc gccccgccgg ccggtgtagc gggtggtggc gagcgcgtcg
    10621 taggtgaagt aggcggagta gacgctgttg aacgcgccgg tggtggcgta cttcttgaac
    10681 tcgttcgggg cgaaccaccg gctgttgatc cactccccga tggccgactc ggcggtgggg
    10741 cggtcgacct ggaacggcag cacgccgtcc acggccaggc gggccggggc gtcgtggacg
    10801 tcgtcgcgct ggatgggtgt ggcgcagtag ggacagcggg tggtggtgag cgtgccggtg
    10861 aaggtggtgt ggccgccgca gttctggcac acgacctcct tctcgtccgc gacctgcagg
    10921 ccggtcttgg tgagcgcccc ggcgtccagg gcggccatgg cctcgtgcag gtcctgctcg
    10981 acgacctcgt cgtcatcgcc gacggcgacc tcctgctcgt tgccgcagaa ggggcacacg
    11041 aggcgctgct tgccgatgtg ccactcgagc tcgccgccgc actgcaggca cgggtaggtc
    11101 cgggtctgct cggtgcggtg cagcagggcg cgctcgcgca ggcgctcggc ggcctcctcg
    11161 gcgtgcccga ccgggggtgg cggaaccacg gcccccgtgt cctccgggag gggcggcggc
    11221 tccggtgtca ttcgctcgtg gtcgggggcg gcgggggcgg cggcgtggcg aacagcggcg
    11281 cgagcgccgg cacctggctg gcctgcttcc actcggccat gccgttggac cacacgaggg
    11341 tggcggcgtt gacctggccg gcggcgacgc cggcctggag ctgctgcacg ctgtagggcc
    11401 cggtggcctg gccgtcgacc tcgacgtgga acagcgcctg gccgggcagc ggcggcggcc
    11461 cggctgcgcc tgcctggccc tggccctggc cggtcgcggc gccgaactgg ttcgccatct
    11521 ggccggccat ggcgatgccc atgcccatgc ccatggcgtc gcccatgacc ccgccgccct
    11581 cgttctcggc ggcggcgagc atcgcgtcgg cggcccgcac ctgctggagg cggccgatgt
    11641 cgccgacgtt gtgggcgtag ccgccctcct cgacacctcg ggcgacgccc cgggtcatgg
    11701 cctgggtgat ctcgtcgggc agcgagatgt tcatcgtcac cgacgtgatc tcgaggccga
    11761 actcgtcgtc cacccggtcc tggacgaact cccggagctt ctcggacagc tcgacctggc
    11821 ggccctgcag gtcgatggcg ccgaggccgg tgtccatgat catgtcgctg aaggccaggg
    11881 tgatgacccg gcgcaacagc tcggtgatct cctcggcctc gacggcggag tcggtgccga
    11941 tgacctcgcg gaggaagacc tcggcgtcgg cgatgcgcac gatgcacagg ccgttggccc
    12001 ggacctgcac catcttgaag tcggggtcgc ggacggtgac cggctgcggg gtgccccacc
    12061 gcaggtcggt gacctggcgg gtgttgagga agtacacctc ggagcggaac gggctgttga
    12121 acccgtgctt ccagccctgc agcgtggaca ggatcggcag gttctcgctg gtgagctcgt
    12181 agttgcccgg atcgaagacg tcggcgatct ggccccggta cacgaacaca gcccgctggc
    12241 cctcgcggac gatcagttgg gcgccgtgct tgatctcgtt ctggtagcgg gggaaccgcc
    12301 aggcgagggt cgagcggctg tcgtccagcc actcgatgat gtcgacgagt tcgcccttga
    12361 gcttgtccag cagtcccacg ggtaccgcct cctgcgccgt tcgctgacgg gggtcgccgg
    12421 tcggcgcgac cggcgcgcag gagcgtattg catcctcagg cgggtgggag cccgtccggc
    12481 aggcggcgtt cgagccggcg ccgcagtgcc aggagcccgg ccgcacctgc ggctgcgccc
    12541 accccgaccg ccgagacgcc ggtgccgccg gtggccggca gcgtcgtcgc cgtgcccgct
    12601 gccgcagggc gcacgcccgg ggtcccatcg ggccggccgc cacccgcgtc gggtcgcacc
    12661 ggcagcgacg tcggcggacc ggcagcaccg tcggccggtc gctgcagcgc ctcgagccag
    12721 ggtccctgga tctcgtaggt gaccccgccc acgaagcccc gctgggagtt gcagtacagg
    12781 cggtctccgg ccgggttgaa cgccaggccg gacacctcgg agtagatcgg caccggatcg
    12841 aggggggagg gcagctgctg gccgtgctgc ggaccggtca tctgcaggat cgggaacagg
    12901 gcgtagtcgt cccgacggat catcaccgcc tgcatgtccc cgccgtcctc ggcgaccacg
    12961 atgttcccgg tgccgggctg gacgatgacg tggtcgaccc cggtgaggac cggttcctcg
    13021 aagtcgccgg cgtcgtagag cacgaccatg gtctcggcga ccacgtcgta catccagacc
    13081 cggtcgtcgc ccttggtcgt gaggaacacc ttgcagtcgt cgtagacgca gccctcgccg
    13141 ccgtcgaagg gcgtcgccgg ggcctgctgg cggcagggca cgccgcgctc gcaggccggg
    13201 tcggccacct cgagccaggt gacccgtccg tcgtcgccga cgctcgcggc ttcgaggatc
    13261 ccgtcgctga gatcggggta gcgggccggg gtgaagcggt agaagcagcc gtcgccgatg
    13321 tcctcggaga ggtagagctg ctgtcgctcc ggatccacgg tggcgttctc gtgcttgaag
    13381 cggccgagcg cgggcaggtc cacggcgggg cgctgaccgg tggggtcgca ctcgaagacc
    13441 ttgcccgagc tggcggggtc gagcgggtcg tccacctcct cgcacgacaa ccaggtgccc
    13501 cacggcgtgg cgccgccggc gcagttcgag cgggagccgg tcaggatcga gtaggcgtcc
    13561 acgatggtcc cgtcgggggc gaagacgatg gcctgggcgc cgccgagtcc ggtgatgccc
    13621 gtcccggtga cgtcgggggc atcgaccggc ggcgggttct cggagttcga cacgaggatc
    13681 catccgccgt cggcggtcgg gaaggtggcg gccccgtcgg ggaagggcat ggccagctcg
    13741 gtgctcggcc cgatgggctc gccgaactgc ttgatcaccc gggccgtgaa cccggcaggc
    13801 agccgcaggc cgttggcgtc agggtccagc agcgggccga acgggctggc gccgggctcc
    13861 gcaggggcgg cgaaggcggg agcgagcgca ccccggccga tggtgaggct gccgctggtg
    13921 gccagggaca gcaggaggaa gcgacgacgg tccacggccg acacttcccg ctcggctcac
    13981 gtcattcctg cctctgggtc agcggagcag cccgaggcgg cggcagcacc ggacgagcag
    14041 ccggttcgac ccggtgatct cgacctcgac gctgtcgacg aacgcccggg cgatcagcag
    14101 cccgcgtccg ttcgaccgca gcgggtcgtc caggtccggg gtctgcggga gcatctgcgg
    14161 gtcgtcggtg cggttcgaga cctcgagcac gagcatgccg gcgtcgatct gcgcctgggc
    14221 gtggacgtcg tcggtgggtt ccggcgacgc agccacggcg ttggcggcca gctcgctgaa
    14281 cacgacctcg agatcgtcga tggtgtcggg gtcgaggtcg gcctcacgca tccacgcgac
    14341 gaagtcccga cggctctccg ggacggaacg gacgtgcgcg tcgaagcgga cggagaaggg
    14401 ttccgtgggc tgaccgctcc gctgcgggtg gctgggcatg cggatccgtt cgggtggtgg
    14461 ctggagctcc cggaccggga gaccggccat ttctacccaa caccgcagcg cctcagtcga
    14521 tccatcccga tttcgtcggc accagggacg gaccgagcag atcggccatg ttctggtagt
    14581 agaagcggtg cttgtcggcc tcggtcacgt cggccatggt cgcctcgaac ttggcgaggg
    14641 gatcccgccc gccctcgggg tgcgggtagt cggtggagaa catgaacagg tcggcgcccg
    14701 cctgctccat catccagccc accggctcgc cggggaacgg ggtgaagcgg aggtgctcgt
    14761 gcacgtactc cgagggcgtg cgcgccagcg tctgcagcgg cgtctcggtg cggcggaagg
    14821 cccgctgggc gaagtcgagc tggtgcatcc aggacacgac ccagctggcc ccctcctcga
    14881 tgacgccacc gcgcaggccg gggaactggt cgaacaggcc gtcgaggatg agggtgccga
    14941 ggaacagcgc cggcgagttg gagatggcga ggaagtcctt ggagcggatg ttctcgccgc
    15001 cgccgaggtg atccgtgacc ggccggccgt tgttgtggaa gctgcggtcg aggagccggc
    15061 cgccgccgcc gacgtggagc acgaagggca ccccgctcgc ctcgagccgc gcccagaagg
    15121 ggtcgaggtc cgggtgggtg ggggctcgct cccccgctgc cgtcgagggc acgtggatgg
    15181 cgccgctgcc gaggtcgatg gcctcctgca ggagggtgac cgcccggtcc gggtcgtgga
    15241 ggggcacgta ggcgaccggc agcaggcggg ggtcgtcggc gcagaaggcc gccaccgccc
    15301 ggttgtgggc ggcgctgccg gcgtagagca ggtcgaggtc cttgccgacg aacatcgccg
    15361 atgcgaacgt ggcgaacacg agctggccgt ggaacccgaa caggtcgacg acgtggctgc
    15421 gctccgccgg gtcgaacgca cccatggcgt tccacccctt gtcgagcagg aggcgctcct
    15481 cggcctcggc acgtgcctgg gcgtccgcct tgcgggcctc ggcatcgccg gtcgccttgt
    15541 cgagcaccgg cgcgagcctc gacatcgagc gccccttcag ccggtcgagc atgtcgtgct
    15601 cgatgtaggg gtcgaggaag ccgggcagct ccatgaggtg gctgtcggcg tcgaggatcg
    15661 ggcgtccccc tgcgtaggtc atcgtcactc cgtcctgtct ggttgcactt tgcaaccggc
    15721 tgtcccgaca ggagagcaca cccggttgca gagtgcaacc agcggtgcgc cacactgggc
    15781 cggtggctgc cgacgtcgcc gggaccgaac cggagcgact gtccgccgac gagcgccggc
    15841 aggcgctcct cgacgtgacc aaggcgctcg tgaacgagtc gggccccgac gccgtcacca
    15901 tgggcacggt cgccgagcgg gccgaggtga cgcgcgccct cgtctacaag cacttcgaga
    15961 acaaggacgc cctgctcctc gagctgtacc ggcgcgaggc cagcgccctc gaccgcagca
    16021 tccgccaccg ggtcgaggcg gccgacgagg gcttcgaggc caagctccgc gccttcgtgc
    16081 ggagctgcct cgacgccact ggggagcacg gccagttctt cgctccgctg cgcagcgccc
    16141 gcaccagcga cgacgcccgc cgccagcagc gccggtggga ccggcggacg tcgggctact
    16201 tcgccgagct ggcggcagag gagttcgaca tcgatccgtc caccgcggcc acggccatcg
    16261 gcgtgctgtt ctccggcatc cagaacctgc tctcccagat gcggcgaagc cccagcgccg
    16321 accggcgaga cgagctcgag gaggtctacg tcgagatgac catcggcgcc ctcgcccgcc
    16381 tggctgccgg ccgtcgacca ccggcgtagg gtctcggtcg acgacgactg ccgggggtgg
    16441 cgggatgcgg cacgggttct ggatccgcaa cgggatggcg cacgaacccg gcgcgagcct
    16501 ggcgctcgcc gaggcagccg aggccggcgg ctgggacggc gtgttcgtga gcgatgccgt
    16561 catggaggac cacgacgagc ccttcgtgct cctggccgcc atggcggcgc gcaccgaacg
    16621 catcacgctc gggacctggg tggtcccgct ggtcgcccgg gatgtggtcg cggtcgcccg
    16681 ggcggccgcc ggcgtcgacc agctctcgga tgggcgcctg gtgctcggca tcggcatggg
    16741 caacgaggtc gagcaccggg gcctcggcgt ggcccgtgac ggcctggggg cgcgccacga
    16801 ggcggccctg gacctgctcg atcggctgct ccgcggcgcg gacgtcaccg tgcacgacgc
    16861 cgatcacgac ctcgacggcg tggcgctgcg cacccgcccg gcccgatcgc cacgcccgcc
    16921 gatcatgctc gccgccacct ggcccaaccg agcgggcacc cgccgggcgg cgcgatggga
    16981 cggcttcatg ccccagtggc ccggcctcgg tgagggcgcc gaccccgacg tggccgaagg
    17041 cgcccgcatc gccgagctgg aggcgctgct gggctgctac cgcgacgccg gcggcgacgg
    17101 cccggtcgcc gtcccccgca ccgagcgctt cggtgccgag tacgaccgga ggtgcgagga
    17161 gctgggcgtg gactggctgc tcacctgcga ctccctggac gaggccgcgc tgcgggccgg
    17221 gccgagcgcg acctgacggc ggaggcgacg cggtgcccgg ggcggggttc gaacccgcac
    17281 gcccgttgcc gggccggggg gtttaagccc cctgcgtctg ccagttccgc cacccgggcg
    17341 ccggcgtcac gatacgcccg ggctgtcatc cactcgcggc ctcagccgcc ttcgtcgtcc
    17401 tcctgcccgg gcggcgcctc ggtcgtggtc gtctgctccg gtgccgtcgt cgtggtcggc
    17461 tccggctcct cctcgggttc ggtggtggtc gtcgtggagg tcgtggtcga cgacgtggtc
    17521 gtggtcgccg acgggtcggg tcggacctcg acgaccgccg tgggaccgag ctgctgcgga
    17581 ccggcaccgg catcgcagcg ggccgagacc tggtaggtgc cgggcacggc cgtgaccggc
    17641 atctgcacgc tcgtccggcg ggtcggctcg tccgagtcca catcgggcgg actctcgacc
    17701 atcaccggct cgacgggcgc gggctcctgg ccctcggggg tctcctgctc ggccaccgcg
    17761 gtcggcccct cgagcacgac gaaccaggcg gcacggcaca tctggtagcc gttggcgatc
    17821 acatcgaccg cctcgccggg ttcgacgctc ggcgtcgcgg tgagctccat cggcggggac
    17881 gtgtcgctca gcagctcggg gtcgggttcg gggaactccg tcggcggcac gtcggcgagg
    17941 gcctcgacca tgaagtcgtg ccagatcatc gccgggatcg tgccgccctg cacgacctgg
    18001 ccgtcgacgt cggggatcgg ctcgttgctg tcggggttcc ccacccacac gatcgccgtg
    18061 tactccgggg tgtagccggc cagccaggcg tccgcgttgc gctgcgtggt tccggtcttg
    18121 gccgccatcg gtcggtcgag ggcggcgcgg cgcgcggtgc cgctccgcgg cgtctcggag
    18181 agcaccgcgc tgacggtgtc ggcgatgcgg gcctcgaccg cgtcctcctc cgtcggaccc
    18241 tccggatccc agatcaggcg cccgtcgcgg ttctccacct cggtgatcac gaagggatcg
    18301 atgctgacgc cggagcgggc gaacgtgctg tacagcgacg ccatctccag cgggctgacc
    18361 tcctccgtgc cgagggcgat cgagggctcg gcctgcagct cgcgccggat gccgaggcgc
    18421 tgggccagct cggcgaccgc ctgtggtccg accttggcca gcgcctgggc gtacacggtg
    18481 ttcaccgagt tcacggtggc gtcggcgagg gtgatgacgc catagtcctg gccgccggcg
    18541 ttggagacgg tccacggtcc gcccgccacg tcgagggtga tctcggccgg cgccgggtac
    18601 tgctcgccgg cgccgttgcc ggcctcgaag ttggcggcga gggtcacggg cttgaacgtc
    18661 gagcccggtt ggcgacccga accgccggca ccgccggcga ggtcgacctg caaggtctcg
    18721 aagtcgcggc cgcccaccca ggcacggacg gcaccggtgc ggtcgacggc gacgacggcg
    18781 gcctggggat cggacggatc gtcgaggtgg gtggccacgg cggcctcggc cgcgcgctgg
    18841 tcggcgaggt ccagggtggt gtgcaccacc aggccctggt cgtacagcgc ctgctcaccg
    18901 agctcctcga tgagcaggcc ccgaacgtgc tcgacgaagt gcggggcgac accctcttgc
    18961 agggtggtcg gcgatgcggc tgcctcggtg acctcgatgg gcgtggcgat ggcggcctcg
    19021 ccctcctccg gggtcagggt gcccatgtcg accatggcgg tcagcacggc atctcgccgc
    19081 tcctgggacg cctcgaggtc ctcgccgggg tcgaggcggg tcggcgagcg gagcaggccc
    19141 atgagcaccg cggcctgcgg ggcggtgagg tccgcagcgg tgctgtcgaa gtacgtccgg
    19201 gcggcggcct cgagcccgta ggagccgcgg ccgaagtaca cggtgttgag gtaccgctcg
    19261 aggatctggt ccttgtcctc ggtccgctcg agcttcacgg cgagcacggc ttcgcggacc
    19321 ttgcggctga gcgtgcggtc gtcgttgagg taggcgttct tcaccagctg ctgggtgagc
    19381 gtggatccac cctgggtgga gtcgtcggtg aggttgttcc acagcgcacg gccgatgccg
    19441 accgggtcga tgccggtgtg gtcgtagaag cgctggtcct cggcggcgat cagggcgtcg
    19501 acggcaaccg gcgccatctc ctcgaggggc acctcgaggc gcaggccctc ctgggccagc
    19561 agggccagct cgttgccctc ccggtcgagc aggaccgccg cggccgacgt cgagcgggcc
    19621 ggcggcaggt cggtcgtcgt ccacagccag gccccggcga ccagcacgag cacgaacagg
    19681 gcggcgaggc cgtagacggg ccagcgcagc cagcgccagg ccggccgggc ccgctcgcgg
    19741 aaggcagcga ggccgctggt cggttcggcg ccggtggcct cgtggccacc gtggtctccc
    19801 ggttcggccg ccgactgctc gacggtgggg tcgtccccgg ggtcgctcgg gtcgcggcgg
    19861 gtgggctcag gagccccgtc gtcttcggtc atcgtggggt ctccttcgac cgccgacctg
    19921 tacccctggc acggtcgtgc cagaactcgc agcgtacgtc agccgccacc ctcagcatcg
    19981 ccgctggcgg tggtcgacgc ctccgtggtg gtgggtgcgg cggtggtcgt cggcgcggcg
    20041 gtggtggtcg gtgccgacgt ggtcgaggtc gtggcgccgc cgccgtcgtc accgtcgtcc
    20101 tcgccgtcgc cgtcgccgtc gccctcgtct tcttcctcgc cctcgccctc gggggcggtc
    20161 gtcgtggtcg acgacgtggt ggaggtggtg gacgacgtgg cccgctggtc ggcgtcgatc
    20221 tcctcccggg cctcctcggc cgccggcgac cggcggttcg ggttgcgcag caggtcgtcg
    20281 tcgggctcgg cgaagtcctc tgccggcacg tcctggacgg cctcgaccat gaagtcgtgc
    20341 cagatcgcgg ccggcacggt gccgccctgc accggtcgac cgtcgacctc gacggtgtcg
    20401 ttgctgtccg ggtgcccgac ccagacgatc gtggtgtagt tcggcgtgta gccggcgagc
    20461 caggcatcgg cgttgttctg ggtggtgccg gtcttggccg ccatcggacg atcgatggcg
    20521 gcagcgcggg cggtccccga ccggggggac tcctgcagca cggcgttcac cgtgttcgcg
    20581 accgccgggt cgacggcctc ctcaccctcc agctcctgct cccacaggac ctctccctcg
    20641 cgggtctcca ccttggtcat gacgaagggg tcgacccggg tgccggagcg ggcgaacgtg
    20701 gagtacacgg tggccatctc caggacgctg acctcctggg cgccgagcgc gatggacggg
    20761 tggggctgga gctcacggct cacgccgagc cgggtggcca tgtcgaccac cgcatcggca
    20821 ccgacctgca ggacggcctg ggcgtacacc gtgttcaccg agcggacggt ggcgtcggcg
    20881 aggctgagcg tgccgtagcc acttccgccc gcgttggaca cctcccacgg ctcggagccg
    20941 gggatgtcga aggtcatctc cgccggcgcc gggaaccgct ggccggcgcc gttgccggcc
    21001 tccaggttgg cggccagggt gaccggcttg aaggtggagc cgggctggcg gccggcgcca
    21061 ccggcgtcgg agaccaggtc gacctgcagg gtctcgaagt cgcgtccccc gacccacgcc
    21121 cgcaccgcgc cgaagccgtc gacgctcacg acggcggcct ggggatcgtc ggggtcgtcg
    21181 aggtgcgccg cgaccgccgc ctcggcggcg cgctggtcgg cgatgtcgag cgtcgtgtgc
    21241 accaccagcc cctggtcgta gagcgcctgc tcgccgaact gctccacgag caggccgcgg
    21301 acgtgctcga cgaagtgggg cgccacgccg gcacgcagga cggtgggggt cgggtcggcc
    21361 ggggtgacct cgaccggggt ggcctgggcc cgctcggcct cagccgggtc gagggtgccc
    21421 atggccgcca tcgcgtcgag gaccgcgtcg cgtcgctgtc gagcagcgtc ggggtggtcg
    21481 tcgggaccga gcgactccgg cgcgcgcagc atgccggtga gcagcgccgc ctgctccgcg
    21541 gtgagatcgg ccgccgtcgt gtcgaaccag gtgcgggcgg cggcctcgat gccgtaggca
    21601 ccgcggccga agtacaccgt gttcaggtac cgctcgagga tcacgtcctt gtcctcggtg
    21661 cggtcgagct tgacggccag gacggcctcg cggaccttgc gggtcagggt ccggtcgctg
    21721 gtgaggtagg tgttcttgac cagctgctgg gtgagcgtgg agccgccctg ggtggaatcg
    21781 tcggtgacgt tgttccacag cgcccgggcg atgccggtgg ggtcgacccc accgtggtcg
    21841 tagaagcggc ggtcctcggc tgcgatcagc gcgtcgacag cgaccggcgc gacctcgtcg
    21901 aggtcgacct cgagccggag gccctcctgg gccagcacgg caagctcctc cccggtgtgg
    21961 tcgacgagca ccgcggacgc ggcgaccgag ggggtcggcg ggagctcggt ggaggtccac
    22021 agccaagcgc cgaccgcgag gacgagcacg aggagggcgg cggcgccgta gacgggccag
    22081 cgcagccagg gccacgcgcc gcggaggccg ccgccaccgt cgcggtgcgg ggccgcctcg
    22141 ggtgcgtcgt ctccgggcgg gttgccggtg gggcgcgccg ggccgggcgc gtcgggatgg
    22201 tggctggaca tggtgggggg ttctccttcg accggcgccc cgtacccgag ccgtccggcg
    22261 agcagacccc gccggcgtca gccgccgacc acgtcgccgc ccggatcgtg gggcacgaag
    22321 ccgtcgaggt cgtcgtcacc ccggaacagg tgccggtggc cgtcgctgaa ccgcacgatg
    22381 gctgcgcccc gcatgcagct gccgtccggt gccgtgaggg ccaggtcgtg gcgggccatc
    22441 gcgagccggt ggtactggtc ggtgcgcagc tgggcctccc agtcggtcgg ccccggtccg
    22501 gtggcgccct cgcggacgac cacgaacacg tcggcgtagt cgaagtcgac ggggatgcca
    22561 tcgatgtcgg cgtgggagag cacgatccgg tacgactgca cgaccccgac ggtagggcac
    22621 cgggtcgctc atgccgccag ggccgcctcg cgctcgagcg ctgtccacaa cgaggtccgg
    22681 caccggatgg ccgcggcacc gtcgaggccg ttcaccagca cgatgccgtc gaccaggtcg
    22741 gccagcaccg cggtccacgg gcggtcgccc acccggaccg ccacggtgca accccgacgg
    22801 ttgcgccgga tggtgcgcac ggcgtcgccc cggcccggcg gcgtgcggaa gatcggcacc
    22861 tcgaggccct gcaggcgggc ggattcgctg agggtccgga cggcgtgggc gaagcggagc
    22921 gacgtggaca tggccccagc atccccagga ccaccgacag gaactcccgg cgcctgcgga
    22981 cccgcgttct tgtcagcacc cctgtgcgac ggtgccgcca tgcccgccct cgccaccgcc
    23041 tggtccccgg cccagcagcg catcatcgag ctgctccgtc gtcgcccgga cgacgcgccc
    23101 gtcgacgccg acgtcgtcgc cgccctccac gccgagctcg aggagcgcct cgcaccggtc
    23161 gccgagcgga tcccggccgg cgcggcgctg tgggtgggca agtcggcgct gcaccagctc
    23221 gacacctgcg aggggcgctg gcaggccgag gccagctccc gcttcgcgtg gaacgtgccg
    23281 gctgcccggg gcacggtggc ccacaaggcg atcgagctgc aggcgaactg gcggggcgag
    23341 agcgagccgt gccgcctggt cgacgaggcc atcacccgcc tcgccgaggg cgagggctcg
    23401 ctcgcggcgt tcctgcgggc cctgtccacg gtggagctcg ccgagctgcg ccaggaggcc
    23461 gtcgggttcg tcacggcgtt cgaggagtgc ttcccgccgc tcaagtcccg gtggcggccg
    23521 gtggtcgacg ggccgtcccg ggtcgccctc gccggcggcc gcatcgtgct cggcggccga
    23581 cccgacctca ccctcggccg ggccgccgac ggcggcaagg tcatcatcga cctcaagacc
    23641 ggtcgcccct cacgccacca cgtcgacgac ctccgcttct acgccctcgt cgatgcctgc
    23701 aaggtcgggt tgccgccgcg ccggctggcc accctctacc tggacaccgg cacgccgacg
    23761 gtcgagcacg tcacccctgg cgtgctccag gccgccaccg agcgggtcgt gcgctccgtc
    23821 gaggcgatgg tggagctgcg gacggccgcc cggcccgccc aggtgcgccc caacccgatg
    23881 tgccggtggt gcccggccct ggccggatgc gagacgggca gcgcctgggt cgccgccgac
    23941 gccgacggcg agcacgaaga cgggttctgg tggccggagc cggaggtcag ccagtcctga
    24001 gctcgcatcc gatctgcacc ctttgggtag gtcgtgtcgc atgtcccatg tgtcagaagc
    24061 tgtgaccggc gaaccgatcc cgacgcgggt gatggagttc gacgagcggt tccgcgacct
    24121 gccgaagcac ttcgctgccg acggcgacat cgtgatgagc cacatcctcg ccgtcctctc
    24181 ctcggtgttc cccgacggcg aggactactt cgtgcgctcc gtcgaggctg ccgtcgacga
    24241 catcagcgac ccggagctcc gacaccaggt cgaggggttc atcgggcagg aagagatgca
    24301 cggccgggag caccgtgcgc tcaacgcgcg cctggccgag ctcgggtacc cgacccacgc
    24361 catcggcgtc tacgtgcgtg ggctcacgtc gctccgggag cgcttccagg gccggcgggc
    24421 caacctcgcc tacaccgccg cactcgagca ctacaccgcc acgctcgccg agacgctcct
    24481 cggcgacccc gacgcccggg cagtggtcgg gcaccgcggt gtccgcgagc tcctgatgtg
    24541 gcacgccctc gaggaggcgg agcacaagtc cgtcgcgttc gacgtgtacc gacacgccgg
    24601 cgggaccgag cggatgcgcg tcaccctcat gtggctcacc cacctcacct tcctgctcga
    24661 gaccagcatc tggaccacgg tctcgctcgc catggacccg gcggtccggc gccgaccgct
    24721 ccgggtgctc gccggcctgt ggcggctccg tcgctccccc ttcgcccggc cccgcgtcgt
    24781 gaagcagctc ttcgactacc accgcagggg cttccacccg accgaccggg acacggacgc
    24841 gctcatcacc gagtggcgga acaccctctt cgggcgcgac ggggatctca ccgagctcgt
    24901 cgccagctga gccggcccga cgacatcacc gaagcgggac gcgacgcgcg ccttccaggc
    24961 gcgtcccgcg gcccagaacg ccaccgtcac ccgagcggca cggcaagccc ggatcttcag
    25021 cccgcctcgg ccgcctcgtc gaaggcgtgg gggtcgtcgt ccatggagcg caggcgcccg
    25081 gtctgcagca ggcgggcgag caccaccagc agcacgacgc cggagaaggc cgtcaccgcc
    25141 cgcacgctca cgacgtcggc cagcgctccc tgcaccagcg agccgatcgg gtagctgctg
    25201 gtgaggccca tcacgtagag ggccagcacc ttgccccggc ggtgctcgtc cacctgcacc
    25261 tggatcgtgg tgttgagcgt cgaggacagc ccgaggtagc cgccgccggc gaccaccagg
    25321 aacagcaccg ccatcgggaa cacgggcgac acggcgaaca gcagcagcga ggtgccgtac
    25381 acgccgagcg cacctccgac cagggtcgag cgggccagcc cgctcccccg gccggccacg
    25441 agcggcgtgg cgaggacacc accgatgccg agggcggcac cgagcaggcc gtagcggaag
    25501 cccgagacct cgaacacctg ctcggcgaac acggccagca gggggaacac cgggttcccg
    25561 aagaagccga gggcgatgac caccacgatg caggtggcga tgcccggcat gttgcgcacg
    25621 taggccgccg tctcgcgcag gtcgccgagg accgaccccg accgcggcgc acgctcgacg
    25681 ggggccggat ggatggtggc cagcgcgacc agcaccgcca cgtacgacag cgcgttcagc
    25741 ccgaacgccc acgacgggcc gagcgtgccg aggacgaggc cgcccagggc cggccccacg
    25801 gcgcgggcgg cgttgaactg cgccgagttg agggtcacgg cgttgagcag caggtgccgg
    25861 ggcaccagct cggacacgaa cgcctgccac gccccgatcg acgtgccggc gatggcgccg
    25921 aacagggcca ccatcgccgt gatgctccac ggctcggcca cgcccgagct ccacaggagc
    25981 cacagcccga cggccgcgag ggcgagggcc gactgggcga cgagcaggac ggtgcgtcgg
    26041 gggtagcggt cggccagggt gccgccgagc ggacccatga acagagcggg caggaactgc
    26101 aggaacgagg cgaggccgac ccaggtcgcg ctgtccgtga gctggaaaag cacgtagggc
    26161 acggtgacgt tctgcatcca cgtgccgctg ttggagatcg tggcgccgat ccagaaccga
    26221 cggaagtccg ggatcttcag cgccgccccc gcctcgcgga ggccgggacg ggcacgctcc
    26281 gcctgcggtc ggtcgatcgc catcgccggg cacgctaacg acgcgtcggc gccgtcccgc
    26341 cccggcacga ccccggccgg gtgtgacatc gccactgcca gaacgcggtg gatacgctcc
    26401 ccgccatggc ccgacccacc gccgagccgg tccgcacgcc cgggcagctc ctcccggtgc
    26461 acgtgcccgc gccggcggcc cccgagctga cggacccgag ggtctccgac gtcgacgagt
    26521 ggggccggtc cgagcgcacg cggcgggcgg cacgggcgct gttcgccccc atgtacgacc
    26581 actggttccg ggtggagtgg gaagggttgg agaagatccc cgccgacggc ggcgcgctgc
    26641 tcgtggccaa ccacgccgcg gcgatcccga gcgacgcccc ggtgatcatg cacggcatcg
    26701 agaccgagct gcagcgggcc gtctacggcc tggccgacga gctgttcaag aaggtgccgg
    26761 tcgtgggcac gctgtggtcc cgcgccggcg gcgtgctcgc ccaccccgac aacgcctacc
    26821 ggctcctgcg ccagcagcag cagctggtgc tcgtgttccc ggaggggtcg aagggaccgg
    26881 gcaagacctt cgacgagcgg taccagctgc gccgcttcgg ccgggccggg ttcgtgcaga
    26941 tcgccatgcg ggccggtgtg cccgtggtgc ccatcgccgt cgtcggtgcc gaggagtcga
    27001 tgccgacgct ggccaacctc gccccgctcg ccaaggcgct cgggctcccc tacgccccgg
    27061 tcaccgccaa catgctggcg ttcggcccgc tgctcggacc gctcggcgcc ctggtccacc
    27121 tgccggccaa gttcaagctg cgggtcctcg atccggtcca cttcgacgtc ccgcccgacc
    27181 agccccggta cagccgcagc cgggtcatgg acgaagccga gtcgatccgc cagcagatcc
    27241 aggacgcgct ctacgacatg ctccgccacc gtcgatccgt ctggagcggg tgatgggcaa
    27301 gcgcgtcctc gtcaccggcc tcggcacgtt ctggggcgca cgcgtcgcca aggccctcga
    27361 agccgacccc gacgtcgacg tcatcgtcgg cctcgacacc cgtgagcccc tgctcgagct
    27421 ggagcgcacc gagtacgtca agagcgacga gaactactcg atcctgtccc gcatcgtgga
    27481 ggccacccag gtcgacacga tcgtgcacac gttcctcgtc gtggactcca cccagatgcg
    27541 gggccggacg atgcacgaga tcaacgtcat cggcaccatg aacctgttcg ccgcggccac
    27601 ggactcctcg gtccgcaccg tggtcgtgaa gtcctcgtcc tacgtgtacg gctgcgagcc
    27661 gagcgatccg gtgtggttca ccgaggagac gccgcgccag aacccgccca agcaccgggt
    27721 cgagcggtcg ttggccgagg tcgagagcta cgtgcgcgac ttcgccgtcg acaaccccca
    27781 cgtcgccgtc accatgctgc ggttctccaa cgtgctcggc cccgagctca gcaccccgct
    27841 gtcacggatg ctcgagctgc cgctggtgcc gcacgtgttc gggttcgacc cccggttcca
    27901 gttcgtccac gaggacgacg tggtgcgggc gatcctgttc gtgctcgaac acgagctggc
    27961 cggcgtgttc aacgtcgccg gcgacggcct gctgccgtgg agcgaggtgg ccgggatctg
    28021 cggcaagcgg accgtgccgc tcccgccgat cggccagggg ctggtcactg ccgcgctcag
    28081 ccggctcggg gtcgacatcc ccccggagat cgtcgacctg ctcaagtacg ggcgcgggct
    28141 cgacaacggg cgcctcaagc gggccgggtt cgactacacg cacacctcgg cgggcgccgt
    28201 gcaggccttc atcgagtcgc tgcgcctgaa gcgcacggtc gggcccaagg ccgagtacca
    28261 gtacgagcgc gaggtcgagg acttcttccg gcgctcgccg gccgtcgtcc gccggccccg
    28321 ctaggagcgg cgggcgacct cggactcgag gtccaggggc tgcaccacgg ctcgggtggc
    28381 gtactcgccc cactcgtcct cgtcgagcag gtacatcagc tcgaactcgt tgccgtcggg
    28441 atcggcggcg tagagcgact tggacacgcc gtggtcggac tgcccacgca gggcgccggc
    28501 cgatgcgagc acctcgcgcg cccgggccag ctcgtggatc gatccgaccc gccatgccag
    28561 gtggtagagg ccggtggcgc cgtggggcgg gcggggtgcg cctgcgccga cgccgatgag
    28621 gccgagatcg tggtggttgc tcgacccgcc ggcgcgaagg aacgcagccc ggccgggcat
    28681 ctcgtccacg acctggaagc cgaggacatc tcggtagaag tcgacggcga cggggacgtc
    28741 gcgcacgtag agcacggcgt ggttcagctc ggtgacgtgg acgggttcca tgttcgctcc
    28801 tctggctcgc ctctggcagt gacgacgggg tccggtcgag ccggaccccg gtcgtcgctg
    28861 gtggtgtcgg ccgccgatgt ggcggccggg ttggatctca gccctcggcc acggcctgga
    28921 cgtcgatcat gatcttgacg ttcttgccca cgagcacgcc gccggtctcc agggcctggt
    28981 tccaggtgat cccgaagtcc tcgcggttga tctcggtctc gccggcgaag gcgatgcgac
    29041 cgttgcccca cgggtccttg ttggcgccct cgaactcgac gtcgagggtc acggggcggc
    29101 tgacgtcctt gatggtgagg tcgccgtcga ccttccagtc gtcgccggac tggcgcacgg
    29161 cggtggagcg gaacgtgacc ttggggaact gctcgacgtc gaggaagtct gcggacttca
    29221 cgtggacgtc gcggtcctcg gcgccggtgt tgaagctggc cgcgtcgagg gagacctcga
    29281 ccgagctctg cgtggggtcg tcggcgacga cgacggtgcc ggactcgatg gcgaagccgc
    29341 cgcggacctt ggagaggccg aggtggcgga cctggaagtc agcgctgctg tggctcgggt
    29401 cgagctggta ggtgccggcg acggggacgg tggtgtcggc ggtggtgcgg gtcaacgtgt
    29461 cagtcattgt tcggtctcct ggggggtctg tgtcagcggg attgttgcct gcacaacaac
    29521 atagcagcac atcgttgcgc gcgcaacgag ttcttcgtaa cgtggtcgcc atggccctcc
    29581 ccaccgatcc cgccgagcac ctcgacgacc cccgcatcga ggcctacggg atgctcatcg
    29641 aggcccacaa cgagctccac aacgccctcc agcgcaacct cgacgcccag gccggcgtgc
    29701 ccgtcggctg gttgagcgtg ctgatccgcc tggcccgctc ccccgacagc cggctgcgca
    29761 tgacggtgct ggcccgggac atgacgatga gcaccagcgg gctcacccgc ctcatcgacc
    29821 gcatggaggc cgagggggtg gtgcagcgcc aggcgtgccc cgaagaccgt cgcgggatgc
    29881 tggcggtgct caccgagcag gggcgcgcgc tgctcgcgtc cgccgctccg tgccacgtcg
    29941 ccgatctcga gcacctgttg ggcggtgcgc tggaccagga cgagctgcac gagctcaccg
    30001 acctgctgcg acgggtccgg gaccacgtgc gccaggtcgg gccaccgccg ggctgagcga
    30061 gagcggtctg ccagactcgc cccgatgagc caggtcacga ccgagatcac cgacggcgtc
    30121 gccgtcgtca ccctgaacga cccggacaag cgcaacgcgc tgaacctcgg catggtcgag
    30181 gaggtgatgg ccgcgatgga cgccatcgag gccgacgagt ccgtcggcgc ggtggtcgtc
    30241 accggcgagc cgccggcgtt ctgcgccggc gccgacctgt cccacctcgg ctcgtcccag
    30301 cgcgacggcc tcatgtcgat ctaccagggc ttcctgcgct tcgggcgctg cccgctgccc
    30361 accatcgccg ccgtgaacgg cgccgcggtc ggcgccggca tgaacctggc cctcgtgtgc
    30421 gacgtgcgca tcgccgccga gcgggccaag ttcgacaccc gcttcctcca gctcggtctg
    30481 caccccggcg gcggccacac ctggatgtac cggcgcatcg ccggtccgca ggccgtcacc
    30541 gctgccgtgc tcctcggtca ggtcatggac ggcaaggagg ccgagcgggt gggcctcgtc
    30601 caccgctgcg tacccgacgc cgagctgctc gaccactgca tcgacatggc ccgcgcctcg
    30661 gccgaggcgc cccgggacct ggtgatgaag atcaaggcct ccatccagga catggccgac
    30721 atcagcgacc acgacgccgc cgtcgagcgc gagctggacc cgcaggtgtg gtcgctgaac
    30781 cagccggcgt tcaaggagcg cctcgccgcc ctgcaggcga agatctccaa gaagtagccg
    30841 ctacaccacc actgccagaa ctgatcagtc ggcgatcacg aagccggcgt cgacgatggc
    30901 caggaccgcg ctctgggtct cggcgtcctc gctggtgacc acgatcgacc acagccggtc
    30961 gtcgctcggc accaggagcc ggctcacgta ggcgtcgatg gtctcgccgt cctcggcgga
    31021 gggttgcgcc aggcggaagt cgatgcggac ccggccgtcg tcggcctcca ccacgttcac
    31081 gtcggtggcg ccggcggcga gcacctcggc ctcggccgcc gtgcgcacgg cctccacgct
    31141 ggccccctcc tcgacgtcga ccttgatctc cgcgaccctt ccctcgtcgt cgacgccggc
    31201 ggcgtagaag agggcgccgg tcgaggccac gttgcgggcc gcgtccagga agaacggttc
    31261 ggccagccgg acgtcctcga gctcggcgag ggcgtcgtcg tcgaggcggg tcgcgttcca
    31321 gccctgcggg accgcgatcc cgaagccgag cgacgggaac ggggcgaacg tggtgccggc
    31381 gatctcgggc acctcgaggt cgcccgggtc ggcctcctcc gtgccgccgt ggccgggcag
    31441 ctccgactgc tccggcagcg gatcggcggg cggcggcgcg tcgtcatcgt cgccgcagcc
    31501 ggcgagggcg gcagcggcga gcccggcagc cagcagcgcg gcgcggaccc tcacgagacc
    31561 cacgcctcga gcagctcgtc gcgccgggtg tcccactcct cggacgtgat gccgtagcgc
    31621 acgtggtcct cccagacgcc gttgatctcc aggtagcgca gggcgatccc ctcctcccgg
    31681 atggcgagct tctcgaccac gcggcggctg gcgcggttgc gggggatgat ggcgatctgg
    31741 agccggtgca ggccgagctc ctcgaacgcg tagcggcacg ccaccacgac ggcttcgggc
    31801 gtgtagcccc ggccggcgtg ggcctcgtcg acccagtacc cgacgtaggc gttctggaac
    31861 gggccccgct gcaccgacga caggttgatc tcgcccacga gcgcgtcgtc ctcgctgaac
    31921 acgccgaacc cgtagccggt gccgagctgc cactcccggg cccgcgccga gcaccgggcc
    31981 gcgaaggcgt ccttgctctc caccacgtcg ggctggccgg cgatgcggcg gggctcccag
    32041 cgggtcagcc agccggtgtt gcgccggcgc acgtcgcgcc aggccgggaa gtcgcggacg
    32101 tcgagggggc ggaggacgat gcggcgaccc tgcaggcgcg tctgcgccgg cgaccagctc
    32161 accggccgag gacttcgcgc agcgcgccga gcagcagcgt cacgttgcgg ggccgggcgg
    32221 tgtggcccat gcagccgatg cgccagacct tgccggcgta gtcgccgagg ccgccgccga
    32281 tctcgatgtc gtagcgctcg agcagcgtgg cgcggaccgc ggcctcgtcg accccgtcgg
    32341 gcacgaccac ggtggtgagc tcggggaggc ggtgcccctc ggtggcgaac agctcgaagc
    32401 cgagctcgac cagaccgtcc tgcaggagct ggccgcactc ggcgtggcgg gcgtggaccg
    32461 cctcgatgcc ctcgtcgagc agggcggtga gcccgccgtg gagggagtag atcatcgaga
    32521 tcggcgcggt gtggtggtac gtgcgcttgg cgccggcggt gccgacgtag ctgccgatca
    32581 tgcggaggtc gaggtaccag gactggctgc gctcgacgaa gcgctcccgg gcccggtccg
    32641 acacggtgat gggcgacagg cccggcggca cgccgaggca cttctgggtg ccggagtagg
    32701 cgaggtcgat gccccacgcg tcggcctcga acgggatgcc accgagggag gtgacgcagt
    32761 cgaccagcag cagggcatcg cccttgcccg cggcgacggg ctcgacgtcg gaccgggagc
    32821 cggtggaggt ctcggcgtgc accatgccga tgagcgtcgg cgaggggtgg gcgtcgagca
    32881 gccggtcggc ggtgaccggc gtaccccagt cgaagtcgac ccggaccacc tcggcgccgg
    32941 tgcgcgacgc gacgtcgcac atgcgggcac cgaacacgcc gttgacgccg atgaccgcga
    33001 cgtcgccggg gccgagcacg ttgacgatgc aggtctccat gccggcggaa ccggtgccgc
    33061 tgatcgggat cgtcagggcg ttgttggtgc cgaacaccgt gcggagctgg tcgcagacct
    33121 cgtcgaggag ctcgaggaac gccgggtcga ggtgcccgag catggggcgc atgaaggcct
    33181 cgatggcttc ggggtacggg ttcccggggc cggggcccat gaggatgcga tcggggcggg
    33241 gcacgccgcg gaggctagat cggttgccgg ggcgagggag aacggctcag gccagggagt
    33301 cgacgaggcc gtcgaggatg tcgctctcgg acaccaggca ctcctcgacg ccgagccagc
    33361 ggacgatggc cacgaagatg cacgccccgg cgacgatgac gtcggcgcgg tcgggctcga
    33421 ggccggggtt gtgcatccgg tcggccatgg actcggtgac cagcgtgcgg aacacgtcct
    33481 cgaccgcgtc ccgctggaag cggaagtggt ggatggcgtc tcggtcccac tcctgcagcc
    33541 cgatctcgac cgccgccatc gtggtgatgg tgccggcgag accgacgaag gtcttggccg
    33601 accggagctg gggcagctcg cgggcgacgt cctccatcca cgcctcggtg accgagatgc
    33661 aggcgtgcag ctcctcgggg gcgggcgggt cgctctcgac gtactgctcg aggaaccgga
    33721 cgcagccgat gtcgatggac atcgagccct cgacctcctc ggtgcccacg atgaactccg
    33781 tggacccgcc gccgatgtcg acgaccagga acggtccgtc ggccggatcg agctcggcgg
    33841 tggcgccgcg gaacgacagc tcggcctcgg tctcgccgct gatgagctcg ggtcgcacgc
    33901 cgaggatctc ctcggcggca tcgaagaact cgtcgcggtt cgaggcgtcg cgcgccgccg
    33961 aggtggccgt gacccggacg cggtcggcgc cgtgctcgtc catgatgcgc cggtagtcgc
    34021 gcagcacctc gatggtgcgc ccgatggcct cggggtcgag cttcccggtg gcgtccacgc
    34081 cccggccgag gcgggtgatg gtgttcaccc gggcgagggg ctccttgtcg gcgccggacc
    34141 cacgggcgac gaggaggcgg gtggagttgg tgccgcagtc gatggcggcg agcacgggtg
    34201 cgtccacccg gccaccctgc cctgccagct ccgcctcgat ccaacgcccg acggggtcgt
    34261 ccccgccggc gaggtgccag gcccaatggg cgtggaagca cttgatgccc cgccgcgtgc
    34321 cgcccacccc accggagggc cgggggccgg tgtggtcggc ggggatggcc gcgtcgcgct
    34381 cggcggcgta gcggtcgtgg gcctcggcga tgacgtcctc gccgatctcg gcctcggccc
    34441 ggtcgatggc gccggtggac tcgaggcggc cgatgcgggc gcgctcctcg tcgcccacca
    34501 gccagtagcg ggtcggcatc ggcgtgccgt cgaacagcag gggcgcgttg cggatcacca
    34561 gcggtgcacc ggcgtcatcg cgcacgacca cgtcgaagtg ggcctgcggc gcgcgcccga
    34621 gcagctcggc gaccgcggcg atgtcggcct cgtcggccac tgcgcgctac gccccgtggt
    34681 tggagcggcg acgacccagc agccagagga cgagcagcac caggaccacc gggccgaccc
    34741 gcttggcgag cgaggcgccg gctgcgtcga cgaggtcgac gggctcgcgg tcgggcgagt
    34801 cgatcttgcg cacgcccgag atgggctcgg ccttggcgcc acccgtctca ccggcgaccg
    34861 gcgtcgcggt ggccccggtg ccggatgcgg cgtcggccgc caccttggcg gtgccgtcga
    34921 ccggggtcgg ctcggcaccg tcgggggtgc cgacggcctg ctcggtgccg gtggccgact
    34981 cagcggcgtc ggcggtggcc gagctgtcga cctcggaggc tgcggaggcg gagctggctg
    35041 cggtgccggc gctggtggcg gcggcagccg gggcgtcgag cttgccctcg aggcagtcga
    35101 cgaactcccc catcagcttg gagctgacgt cggcgatcat gcctcgcccg aactgggcga
    35161 ccttgccctt gatggtgagg tcggtgtcga cggtgacgtg ggtctggccg gactgctcgg
    35221 acatgttcac ggtgatcagg gcgctggcgc tgccctggcc ccgggtgtcg cggccctcgg
    35281 ccttgagcac cagcttgcgg gcggcctcgt cctgctcctg gaaggtggcg gcgcccttgt
    35341 actgggccgt gatcgggccg accttgacct tcacgatgcc gcggtactcg tcgccctcga
    35401 cctcctgcag ctgggcgccc ggcaggcacg gggcgatcag ctcgacgtcg gtcaggacct
    35461 cccatgcccg ctcgatcggt accgggacgg tgaactcgtt ggtcagctcc atctgcggag
    35521 atcctccagg gtgtcgatgt ctgcggtgcg atcggtgcgg gtgtgcccgg cgtcgcacgg
    35581 tatctccatc acgagctcgg gacgacctcg catcacggca cgcccgacct cgtcgccgtc
    35641 gactggcaac aggtcccaca cctcgtcccc gaggcggacc gggtgcccgc gccggccgtc
    35701 gtaggtggcg acggcaacgg cggcatcagc cgcagcgacc gcctgccacg ccgccgcggt
    35761 gacgaagggc tggtcgccga ggccgaccac gacagcgtcg tgaccccgtc gacgggcctc
    35821 ctcgatcccg acccggaccg atgtcgcctg gcccgcctgc cacgccgggt tcgacagcgt
    35881 ggcgatgccc tcgggcacga ggtgggcgac atccgccgcc ccggtcacga ccaccgcctc
    35941 gagcccgctg gcccggagcg cgtcgatggc gtgggcgagc accgggcggc cgtcgagggg
    36001 ggcgagcagc ttgtgggtgt cgccctcgta gcggctgcca ccgccggcag cgagcagcac
    36061 ggcgaggacc atcgccccag tgtggccggg cggcgcctgc gctggtcgcg tggtcgtggc
    36121 agcatcggcg catggatccc ctcgccgccc gccgcaccct cgcccaggtc cgcctcgcca
    36181 tcggcgtcgg cgcgctcgtg gcgccccgcc tcaccgggaa gctgttcggc atcgacgtcg
    36241 ccgccaaccc ggcggccccg tacctggccc ggctcttcgg cgctcgcgag ctgttcatgg
    36301 ccgccccgtt cctgatgccg gccccgggcc tcgacgaggc cgagctcgcc tcccgcgccg
    36361 tgccggtcga cgccgccgac gccctcgcct ccctggccgg cggcctccgg ggctacctgc
    36421 cgtggcgggc cgccgtcccc gccaccgccg ccgggctgct cggcacctgg ctcggctccc
    36481 aggccgccaa gaacgagaag ctcatccgcc ccacgtgatc acccgttccc tgcgcgcaga
    36541 acgacctcac gcggtcggat cgcgcgcaag gaacgatgag ggtctcgttc cctgcgcgcg
    36601 gaacgacgtc agacggtcgg atcgcgcgtg aggaacaggg tggtggccgt cagtggatgg
    36661 ggccggcagc gtcgcgcagc gacggggccc ggcgtccggt gcgggtggcg atgacctcgg
    36721 ccatgatcga caccgccgtc tcctccggcg tgcgtgcgcc gaggtcgagc ccgatggggc
    36781 ccatcacccg ggccagctgc tcgtccgtga cgccctcgtc ccggaggcgg cggttgcggt
    36841 cgtcggtggt gcgccgggag cccatcgccc cgaggtagcc cacgtcggtg cgcagcgcgg
    36901 cgatgaccgc cgggacgtcg aacttcgggt cgtgggtgag cacgcagacc gcgtcccgcg
    36961 ggccgagctg gtcgcccacc cgttcgagca gccggtgggg ccagtcgacg acgacctcgt
    37021 cggcgaacgg gaaccgggcc ttggtggcga agacctcccg ggcgtcgcac accgtcaccc
    37081 ggtagccgag caccttgccg acgcgggcga gggcgccggt gaagtccacc gcgccgaaca
    37141 ccagcagctg gggcggtggg gcgaaggact cgacgaagac ggccacgtcg tgctcgttgg
    37201 cctgcccctg ggtgccgtag tggcgcaccc cggtggtgcc ggcggccagc tcgccgaggg
    37261 tgtcgcgcgc gacgacgcgg tcgaggtcgg cgtcgcccag cgaaccgatc ggctcccggt
    37321 ccggtcggac gagcagcttg ccgccgaccc cgggaccctc gaccaccgtc gccagcgcca
    37381 ccggctcctc tgccgcgatc gcgtcgcgca gcacctcgaa gagcgcgatc accagtcgag
    37441 cggttcgatg aagaggtgga tggtgccgcc gcaggtgagc ccgaccgcga aggcctcgtc
    37501 gtcggagtag ccgaaggtca cgatgcgccg gtcgccggac tcgatgatct gcagggcctc
    37561 ggtgaccacc gcaccctcga cgcagccacc ggagaccgag ccggccacct cgccgtcctc
    37621 gttcacggcc atggccgcgc cggggaggcg gggaccggag ccctcgacgt cgaccactcg
    37681 cgccacggcc acctgcttgc ccgccttccg ccagcggtcc aggtccccga ggatctctcg
    37741 cacgccggcc agcctacgga gcgacggcgg ggacgggggc ggcctcgccg gcgatgaccg
    37801 cggccagccg ctcgagcgcg tcgatgctgt gcccctcgac gaaggcatcc acgtggggca
    37861 gcgcggcggc catcccccgg gcgatcgggg cgtagccggg cgtggccttg agggggttga
    37921 cccagaccac gcggtgggcg acgcgctgga ggcgctgcat ctgctcggcc agcacctcgg
    37981 ggtcgccacg gtcccagccg tcggagagga tcaccacgac cgcgccccgt gccatgccgc
    38041 gcacgcccca ctcgtcgttg aaccggccga gcccctcgcc gaggcgggtg ccgccggcga
    38101 agtcggcgac ggccgctgcg gccaggcgga gcgcctcgtc cggatcccgt cgcctgagct
    38161 cccgggtgag ccgggtgagg cgggtgccga gcgcgaacgc ctcgatccgg ctgcgcgccg
    38221 cgacggcggc gtggacgaag cgcagcagcg gtcgggcgta ggcctccatc gagccggaga
    38281 cgtcgaccag gagcaccacc cgccggaacc gctcgcccgg ctgggtgtgc gaccgccgga
    38341 tcggctcccc gcccgtgcgc agcgcgtggc ggaccgtgcg gcggaggtcg gggcggcccc
    38401 ggacgcccct cgtcggcacg ctgcggcgtg acggccgcgg cgctccgacc agggccaggc
    38461 ggtccatcag cgcccacgcc tcggccagct cgtcgtcgtc gtaggcggcg aagtccttgt
    38521 ggcgcagcgt ctcgacccgg gagaagcgca cctgcacggt ggggtcggcg tcggcgacgt
    38581 cgtcaccgcc gtccatctcg gcgtcggggt cgtcgacggc gagggtgatg cgctgggggc
    38641 tggatgccgc cggccggggc aggggcacgc cgccgaagaa ggcggcgaag aggtggtcgt
    38701 aggcggcgac gtcgtcggct cgtcgcacga gcgtggaccg cccggcccag tacacgtcgt
    38761 cgccggtccc gaggtcgacg tggtcgaggg cggcggcgaa cgtggcggtc gcgtcgatgg
    38821 ggacgacgag cccggcctgg cgcagggccc ggacgaaggc cacggcgctg gtggccggat
    38881 cgcgggcgat gtcgaccatg ccgcggctca ggcgctgcgc tcgacggccc gggccacgag
    38941 gccgtcgagg cccgatgctc gtacccgcgc ttggtcctcg cggtacttga gcaccgtgcc
    39001 gagcgacgcg tcgaccgtct ccacgtcgag ctcgctggcc ccgagcacgt gcagcgctgc
    39061 ggcccagtcg atggcctcgg cgacgcccgg cggcttgtag agcccggctc cccgcagcgc
    39121 ctcggtggct gcggcgacct ggcgggcgag gcgctcaccc acctccggga cccgcacgcc
    39181 gacgatggcg acctcgcgct cgaagtccgg gtgctccacc cagtggtaga ggcagcgccg
    39241 cttcagggcg tcgtggacgt cgcgggtgcg gttggaggtg atgaccacga gcgggggcac
    39301 gtcggcgtgg aaggtgccga gctcggggac ggtgaccgtg tagtcggaca gcacctcgag
    39361 caggaacgcc tcgaactcgt cgtcggcgcg gtcgacctcg tcgatcagca ggaccggggg
    39421 cggacccgac ctgggcgtca ccgcctcgag cagcggacgg cgcaccagga agcgctcgtc
    39481 gtagagctcg tcctcgagct ggtcgacgct ggccccgccg gcgacacccg ccgcctcggc
    39541 ggcgcgcagg tggagcagct ggcgggagta gtcccactcg tacacggcct ggctgacgtc
    39601 gagcccctcg tagcactgca ggcgcagcag ctcgccgccg gtccagcggg agagcacctt
    39661 ggcgacctcc gtcttgccga caccggcgtc gccctccagc agcagcggtc gctggaggcg
    39721 cagcgccagg aacacggccg tggccaggcc cgtgtcgggc aggtagtcgt gggcctcgag
    39781 cgcggccgcg acgtcacggg cgtcccggac gtcggccgga ggtggtgcgg acggggtcac
    39841 ggtcgcaggc tacggtccga cgaccgcacc acgaggggga accatgggac tgcgacacat
    39901 cgtcacgctc accttccggg acgacaccac cgacgaccag atccacgaga tcgtcgaggc
    39961 gctgcgccgg ctgccgtcgc agatcgacga gatccggagc tacgacgtgg gcaccgacgc
    40021 cgagctcgcc gagcgcaacg cccacctggg catcgtcgcc gacttcgact cggtggaggg
    40081 ctacgaggtg taccgcgacc acccggcgca ccaggccgtc atcaccgagc tcatcgtccc
    40141 cgtgatcgcc ggccgcagcg ccgtccagca ccacatctga ggccggccgt ggacgacatc
    40201 gccttcatct cccgccccga ccaggtctcg cccgagtggc tcaccgcggt gctgcgcgcc
    40261 gccggtgcca ccgacgaggg caccgtcgtc gacgtgaagg ccacgccggt cggcacgggc
    40321 caggtcggcg acagcgtgcg cttcgccctc agctgggacg acgccgacgc cggcccggcc
    40381 acggtggtcg gcaagttccc caccgaggac gagaccagcc gggccaccgc cacggccgtg
    40441 cgcacctacg aggtcgagac ccgcttctac cagcagctcc gcggccgggt ggacatcgcc
    40501 gcgccggtgc cgttctacgc gcagatcgac atggcgagcc acgacttcgc catcctcatg
    40561 aacgacctgg ccccggccga gcagggcgac cagatcgccg gctgcacgcc ggacgaggcc
    40621 gccatcgccg tcgaggaggc ggccaagctg cacgccccgc tgtggggcga cagcaccctc
    40681 gccggcctcg actggatgaa ccgcaactcg cccgaggggc tccgcggcct ggtcgacatg
    40741 gtgtggcccg ggttcctgga gcgctacgac ggccgactcg acgccgaggt ggtcgacgcc
    40801 ggcagcgtcc tggtcgagcg cctggaggcc tacctgtcgt ggcggcccga gcagctcacg
    40861 gcggtccacg gcgacttccg cctcgacaac atgctcttcg acccctcccc cggcggcgta
    40921 cccatgacca ccgtcgactg gcagaccgtc agccacggac ccggcgccca ggacgtcgcc
    40981 tacttcctcg gcaccggtct cgacccagac ctccgggccg aggtcgagca ggacctggtc
    41041 aaggagtacc tgtccacgct gcgggccggc ggggtgaagg accagtcctt cgaggcgtgc
    41101 tgggaccagt accgccgctc cgcgttcgcc ggcttcatca tggcgatgat ctcgtcgatg
    41161 atcgtgggcc agaccgaccg tggcgacgag atgttcatgg ccatggccaa ccgatccgga
    41221 cgcatggccc tcgacctcga caccctctcg atgctctagg tcgttccgaa ctggcagtgc
    41281 tggtgtagcg atcaggccgg gtcgatcggg agtccggact cggaggggac gtcgtcgagg
    41341 agcagcacat ccacgtcgcc acccgccttg actccgtccc cgtcgggcag gacggcgagc
    41401 gcgttcgcgg ccgccatcgc gccgagctgg tggctgccct ggccgccggc ggagtcgacg
    41461 tggacccgac cgtcggcacc gtgtcgggcc acgacgcggg cgaagtgggt cttgccgtcc
    41521 ggtcgccggt cgaacgccgc atcggccacc gctcgcacga tgggccggtc gaggtcggcg
    41581 tggcccatcg cctggcgcag ggcgggacgt ccgagcagct cgaaggacac catcgacgag
    41641 accgggttgc ccgggaaccc gaacaccggc acgccgtcga ccagcccgaa ggcgaacggc
    41701 ttggccggct tgatcgcgat ctgcatccag cgcatgtcgc cgatccggtc gaggatcacc
    41761 ttcaccaggt cgatgtcgcc catcgacacc ccgccgctgg tgagcacggc gtcgcactcc
    41821 gagacgcccc ggcggatggc atccgtgatc gcctcctcgg tgtcgggggc gatgccgagg
    41881 tcgaccacgt cgcacccggc gccggcggcc agcgccagca aggtgcggcg gttggagtcg
    41941 cggatctgcc ccggttcgag ggactgaccg ccctcgacga gctcgtcgcc ggtggacacc
    42001 acgcccacgg tgagacgggg atgggccagc acggactcca cgccgaggct gcacaggaca
    42061 ccgaggtggg cggcggacag cacctcgcca gccgccagca cccgggcgcc gacgtcgagg
    42121 tcgtcccccg caggcctcac gtggttgccg accgggacct cgatgtcgac gcggacgtgg
    42181 ccctcatcga cgaggtcggt gcgctcgacc atcacgatgg cgtcggcacc cggggggatc
    42241 ggcgcaccgg tgaagatccg gctggcctgc ccggggccga cctcggccgt cggcgcgtgg
    42301 ccggcggcga tggtctcgag gatctccagc tcgacgggcg cgccgaccgt gtctgcggcg
    42361 cgcagggcaa agccgtccat ggccgtgttg gcgaagggcg gcacctgctc gctggccacc
    42421 acgtcctcgc ccagcacgag gccgagggcg tcgccgacgg gcaccgggac cggggcccgc
    42481 cggggcaccc ggtccagcac gtgtcgtcgg gctgctccga gggggatcac cccgccgacg
    42541 gtaccgctca gcaggaagcg gtcagctcgc ccgccacccc gtcgccgagg ccctcgtcac
    42601 cctcgaagaa ggtgcccgtg ccggtgacgg tgctcccgtc cagctccagc tccacgtcgc
    42661 ccatgacgct cgaggcggtc cagacggcct cgtaggtctc gctgtcgtag acgtccatgc
    42721 tcgccatgcc cggcatcacg gcgtcctcgc cgaacttggt ggcatcgaag ccgtacggct
    42781 cgtcgtacga ggtcaccgag aacaggatct cctggtcggc agccatctgc ggctcgagcg
    42841 agcacaggac cgagaactcg aactcctcgc cgttggccag gaccagggtg gccgtgccgt
    42901 ccccgcccga ggtcccctcg ccctccgcct catcggctgc gccctcgtcg tcgccggtcg
    42961 cctcgtcgcc ggtctcctcg gcatcgttgg cgtcgtcgag gctgtcgtcc tgaccgacgg
    43021 catcggccgc cgcatcgtcg tcgtcaccgc aggccgcgcc gaggagggcg agggcgagga
    43081 cgggggtcag cagggtcttg gtgcaccgcg tgaacatggc agggactgtg ccacgaacgc
    43141 gacgcccggt cgcggaggtc agcccagcgt gtgcgtgcgg atgaaggtga cggcctgatc
    43201 gagtgcgtcg gacgcctcgg gcacgagggg cgcctgggtc tggaagatgt gccagaggtc
    43261 gtcgtgcacg accagctcgg catcgcctcc gacttcacgg acccgctcga cgaggcggcg
    43321 ggcgtcgtcc tcgagcacct cggcagcggt cgcctggacg tggagcgggg gaagccccca
    43381 cagctcggcg aagagcggcg agatgcccgg gtcggacggg tcccggtcgc cgacgtagtg
    43441 gtcgcgccac gactcgaaga gggcaggctc gagcatcagc tcggtggacc ggttccgctc
    43501 catcgagtcg cccgagaacg tcaggtcgac ccacggggag accagcacgg cagcgttcgg
    43561 cggcgtccag ccctcgtccc tggcgcggat catcgtgccc aacacggcgt tgccacccgc
    43621 ggactcgccg atgagcgcga ggctctcggg cgccacctca tcggcgacca cccggtacac
    43681 ggcgaggcag tcgtcgatcg cggccgggta cgggtgctcg ggggcgaggc ggtagtcgac
    43741 cgacgtcacg accgcatcgg cgcgcagcgc cagctcggag aggtaccccg cgaagccccg
    43801 ggtcgtcccg acgacgaagc cgccgccgtg gacgtgcagg aggtggcggc ggcggggcga
    43861 gcccttgggc gacacccgca ggcaggggac gcccccgacc tcgaggtcga cgacctccac
    43921 ccggcggttg gggagcccca gcgaccgcga gagcttggcc cgctgctcct cgggcgtcag
    43981 ctccggcccc ggcatgatct tcgggatcag gcgttggacg atgcgtgctc gcaggctcag
    44041 cgacatgggg cggagcgtag cgacggtgcg cgcgcctccc gcccgggtca ggtccgctcg
    44101 aaccaggtcg cccgggcctg ggccaggacg gtgccgtcgg tggtcgcgag ggcggtgccg
    44161 gcgaagagct tgcgcccgtc ccggtcgatc tcccagccgg tcacgatgtg gtcgatgccg
    44221 acctcggccg gtgccagctg ctcgaagacg atccggccga ggaggccggc gaaggtgccg
    44281 cgggcgatga aggccatggc ccccgggcag tccagggcgg cgagcaccca ctgtgggtcc
    44341 acggttccgt tcggaccggc catcgcggac gtcggacgcc acacggcggc gacctgctcg
    44401 tggccgtcga cctcgagcgg tccgacggcg atctcgaggc cgtcgtcgtc gtcccgggcc
    44461 agaccgcagc cgaggcagat cgggtgcaca ccgagctcgt tccagtggga gggcgacccc
    44521 ttctcggccg ccagggcggc gtcgatgtcg ggcaccggcg gcacgtcgag gtcgagattg
    44581 gcggcctcag cctccgcgat cagctcgccg tcgtggtgca gcacgacccg cccgtcgtcc
    44641 tcgacgtcca cgtccaggtc cacgcccacc ggggtggccc ggcgcagcgt gacctgcacg
    44701 gcgccgtcga cgtgccgggc gaggaggccg gcgacgtagc cgccgtggcc catgcccggc
    44761 gggccgacga agtgctcggg gacgacgacc gtgctcacga cccaccaggc aagcaggccc
    44821 acctcccgtc cggcgaaccc tcagtcgagg gtggcgacga agcccttgag gtactcccgg
    44881 aacgcagggc cgaggtcgtc gcgcatgcag gcgatctcga cggtggcctt gaggtagtcg
    44941 agcggcttgc cgcagtcgta gcggccctcg cggaacgagt acccggtgac cggctgggtc
    45001 ttgttgagca ggccgatggc gtcggtgatc tggatctcgc cgccgacgcc gggctcggtc
    45061 tggcggatgg cgtcgaagat gtccggcgtg aacacgtacc ggccgatcac cgccaggttc
    45121 gagggcgcct cctcggcgga tggcttctcg acgatgcgct cgaacccgta gaggttgtcc
    45181 cggatcggga tcgggtcgat gcagccgtag ctggagatct cctccggctc cacctcgagc
    45241 agggcgatga ccgacccctg ctgctcctcg tgcgtctcga tcatcccgcg cagcagggcg
    45301 ccgccgtcga cctggacgtc gtcgccgagg agcaccgcga agggttcgtc gccgacgtgg
    45361 cgctcggcga tgccgatggc gtgcccgagc ccgagcgcga ccccctggcg gacgaagtgg
    45421 atgtcgcaca gctcggccag gccccggacc atctcgagct cggccgtctt tcccttggcc
    45481 tccagcgcgg actccagccc catcaggcgg tcgaagtggt cgccgatggc cgacttgccg
    45541 ctgctgtcga tgatgaggac gtcctcgatg ccggcggcga cgcactcctc gacgatgtac
    45601 tggatcgtcg gcttgtcgac gaccggcacc atctccttgg gaatggcctt cgaagcgggc
    45661 aggaagcggg tgccgaggcc ggcggcgggg atcacagcgg tgcgcacgga ggtcatccgc
    45721 ccttcctagc cgacgccccc acccttcatg cctggcctcc gggcgcccgg ccgcggtccc
    45781 ggaccaccga ctcggtgccg tcgaggaggc gggcgatgtt ggtgcggtgg cgcacgacca
    45841 cgaccagccc gatggccgcc gccacgacca cctcgtacca ccgctggccc cgcaccagca
    45901 cgccgagcgg gagcaggacc gcgagggcga tcgaaccgac ggcagccgtg cccgtcacct
    45961 tcaccagcac cgcgaagacc acggcgagcg ggagggcgac gagcggtgcg cacaccagcg
    46021 ccacgccggc tgcggtggcc acgcccttgc cgccgcggaa gcgccgggtg accggccaca
    46081 ggtgcccggc gaccgcagcg gcgccggcgg cccaggcgag cgcccggccg tcgaccacgt
    46141 agcccagccc ggcggcgagc atccccttgc cggcatcgcc gaggaggacg acagcgccgg
    46201 cgcgcatgcc cccgacgcgg gtggcgttcg acgccccggg gttgcccgag ccggtcgtgg
    46261 tcgggtcgaa gccgtgacgt cggccgacca gcaccgcggt ggggaacgtg ccgaggacgt
    46321 agccgacgac cacgacggcg acggccagca cggacatgcg gcgagcgtag gcggatcggt
    46381 agcgttggca ctcgaacccc ttgagtgcca ggacctggag agctgcgatg cccacctacg
    46441 agtaccgctg caagagctgt ggccacgaga tggaggtcca gcaggcgttc agcgacgacc
    46501 ccctggacac ctgtccggag tgcgagggac ccctgcgcaa ggtgtacggc aacgtcggca
    46561 tcgccttcaa gggctccggc ttctacaaga acgacgcacg cgggtcgtcg aagaagtccg
    46621 cgtcgtcgtc cagctcgacg tcggacacgt cctcgtccgg gtcgggctcc acggagaaga
    46681 aggccgacac caagaccgag accaaggcca cgcccgccgc ctccagcagc gactgacgcc
    46741 gcccgctacc gtctcccctg ccggcccggc cggcgcaccg ggcgacgatt cgtcgacgaa
    46801 tcgccgccca cggacttccc ccgagcgctc cgtcctccgg aggtccccgt gcgtcctgcc
    46861 cccgcccgct atccgatcct gcggccgccg ccgctccgac tcgtgctcgc cgtggtgctc
    46921 gcgctcgtgt gcggtgccct ggtcctgcgt gccaccgacc gggccgaggc cgcagtcgac
    46981 cggtacggca gccacacgac ggcgtgggtc gtcgcctccg acctcgaacc cggcgacgtc
    47041 atcggctccg tcgacgtcga cgccgagtca cggccgatct cgttcgtccc gaccggcgcg
    47101 gtggaggccg acccgaccgg gcgtcgcgcc accggtgcgc tgcagcgggg cgaggtcgtg
    47161 gtcgagcacc gcctcgccgg cggcgaccgg cgcggtgcgg ccgcgctcgt cccggccggc
    47221 tggcgtgcgg tggccatccc ggcgctcgag gccgccctgc cggtggaagt cggccagcgg
    47281 gtggacgtgc tcgccgcgct cgacccgtcc ggcacgggtg cgaggggcag cgtcgtggcc
    47341 gaagacgggg tcgtggtcca cgtggccgac gacgacacgg tcaccgtggc cgtgacgccg
    47401 agccaggcga cgcgggtggt cgccgccctc gccaccgggc tggtcgccct cgccctggtc
    47461 gggtgacctc acagcgccgt ccggggcacc ggtacggttc gcggcatgtg cagcaggtgg
    47521 tggcgggcgc tgctcgcggt ctcgttcgcg ttcgcgtcgg tgctggcggc gccgacgccg
    47581 gtggccgccc aggaggtgcc cgccgtgacg acccacgtgg tcgattcctt cgacggcaca
    47641 ccgctgctcg ccaacctgtt cgtcccgtcc ggggtcgacg ggtcccggcc cgcaccgctc
    47701 gtgctgctga cgcacggctg gggcggcacg ggccagaagg cgcccaccgg cttcgtcgcc
    47761 gacctcctgg acgccggcta cgtcgtggtc acctgggacc aacgagggtt cggttgctcc
    47821 ggcggcgagg tccagatcga caagcccgag tgggagggcc gggacgtgca agccctcatc
    47881 gactgggcgg tggccaacgc cccggtgcag ctcgacgcca ccggcgaccc gctcgtcggg
    47941 atgaccggcg gctcctacgc cggcggggtc cagaccgcgg cggcgtcggt cgacgaccgc
    48001 atcgacgtgc tcgcaccgga gatctcctgg gcggacctgc ggtactcgct ctacggcggc
    48061 caggtcgtga accagggatg ggccacgctg ctctacctcg ccgggctcgc cagcgcgtcg
    48121 ggccagggcc tggacccgag ctgcccgacc cacccccaac ccggcgggct ggacccgatg
    48181 atcacgcggg gtttcaccga gggctcgacg acggcggcca tctccgacga ggtgctcgac
    48241 ttcttcgccg gcagcagcct cgcctcgtac ggccaggacc aaccggtcga catccccacc
    48301 ctggtggtgc agggcagcac cgacaccctc ttcgacctca ccgacggctg gcggatcgtc
    48361 gagcacgtgc gggccaccgg tgcgcccacc cgcttcctgg tgttctgcgg tggacacgtc
    48421 gcctgcccgg acacctacgc cgacgccgga gacggcgagc acgtcgacgc cgccgtcctg
    48481 gcctggttcg cccggcacct gcgcggcgac gacaccgtcg acgtcggccc gccggtcgag
    48541 taccgcacca acgagggcgt ctggcgggcc acgaccggct tccccgcgcc cggttcgccc
    48601 ccgctcgagg ccacgggcgc cggctcgctg gtgagcgtcc ccaacgtgga cgccccggac
    48661 ctcagcaccc tcggcggcgc ctccttcgac ggcggcatcc cggcgctgcc cttcacgtcc
    48721 gccgccgtgt ccgcgcccgg agacatccgc gccatgaccg tcgaggtcgc gcgggccacc
    48781 gatcgcgccg tcgacctgct cggcatcccg accgtgaccc tgcaggtcga gggcgtcggc
    48841 gacgaggtca tcctcatggc caagctggtc gaccgcgagt ccggcgcggt ggtcaacctg
    48901 caggagggtg cggtccgtgt gccgctgctc gacggcgggg cccaggtcga ggtgcccatg
    48961 cccggcgtcg cctacacgct gccggccggc caccacctgg acctgcaggt gagcaccgcc
    49021 agcctgatgc acagcaacgc ccggacgccc gcgacggtcg acgtcatcgc caccgtctcg
    49081 gttccggtcc aggccgtcga ccgtgccgca gcaccggccg gtccgacgag ccccgctccg
    49141 gcgccgaccc cggccgcccc gccggtgacg ctgcccgcca ccggtgccga gacgtcgggg
    49201 accgtcggtg cggcgctggc cgcggccgtc gccggcgtgg gcctgctgct cgccggtgcc
    49261 gccgtgcgcc ggcggcgggg gctcagctgt tgaagccggc gaccgcgagt gcgatgacgc
    49321 cggcgccgac cacgaagcgg tagccgacga agggcgcgaa ggtccgggtc tggacgaagc
    49381 ggatcgtgcc ccacacggca gcgaagccgg tgaccgcgct cgtggccatg ccgacgagga
    49441 acggggcgac gaagtccgac gggatgccac cctcgcccac gacgtcgagc agcttgtaga
    49501 ggccggcacc ggcgatgatc ggcaggctca tcaggaacga gagccgtgcc gcagcggcgc
    49561 ggtcgaagcc gagcgcccgg ctcaccgtga tggtgacgcc ggagcgggag acgccaggct
    49621 ggagcgccgc agcctggccc aggcccatca gcagggcgtc gcgcagccgg aactcgcccg
    49681 cctcgcgcgg tccgcccatg cggtcggcgg cccacagcag gacgccgccg acgatgagca
    49741 tcacgccgat cagccagaag atgtcgtcga ggtcctcgat gacgtcgttg agcaggaacc
    49801 cggtgatcgc cgccggcacg gcagacacaa cgagcagcca ggcgatgcgg ccctcgggcg
    49861 gggcatcggc gcggttggcc ggcaccacga cgcgcaggcc ctcgcgggcg aagacgatca
    49921 ggtcccgcca gaagtaggcg atggcgccgg cgagggtgcc gatgtggagg gcgacgtcga
    49981 aggccttggc cagggagtcg tccccggcga agtcgtccca accgaagagc cacgggacga
    50041 tggcgaggtg cccggacgac gagatcggga tgaactcgga gaggccctgg acgatgccga
    50101 ggacgatggc gtggaggagt ggcatgaggg agggcgttct ggcctacgag atcccctgcc
    50161 ggtgcgggga tgtgcaggcc agaacggaag ggaccgttct agcggccgga cagggacacg
    50221 ccgggaatca gcagggtcac gcccccggtg ccgccgggct gccattcgac gtcggcgccc
    50281 acgtgggaca cgtcgaggag gaggcgctgg agcgtgctgg ccagcgtggc ctccttgatc
    50341 ggctcggccc gctcgccgtt gcgcacccgc agcccctcca ccgacaccga gaagtcaccc
    50401 gagaccgggt tcacgccgga gtggatgccg ccgagggagg aggcgagcac gccgtcgtcg
    50461 accgtggcca gcagcgagtc gaggtcgccc tcgcccggct cgacggtgag ggcccgggcc
    50521 gagatgcccg gccggctgct gtagctgcgc gccgccgagg cggtcgaggt cgtcccggcc
    50581 cggcgggccg agtgggtgtt gtgcaggaac ccctgcagca cgccctgctg cagcaggggc
    50641 acgcgccggg tggccaggcc ctcgtcgtcg tagggctcgg cgccgaggga gtcggggttg
    50701 gtggggtcgt cgacgatggt gagcagcggg ctgccgacct gctggccgat gcggttcgca
    50761 aacgggctcc gtcccttggc cacggcctcg ccaccgagca tgcccgagac gatgccgagc
    50821 accgtggcgg tgagacgcgg ctcgaggatg agggtgacga cggccgactc gggcttgacc
    50881 gagccgatct tggccagcag ccggctgacc gcgtcgtcgg cggcctcgtc gagggagacc
    50941 tcgtcgatgg cccgcccgac cgacatgccg ccggcgatga ccgagacgtc gccgtcgtcg
    51001 gccagcgccg acaccgagac gtaggcgatg gtcgagcggc caccgacgga gattccggtg
    51061 gacgtggcca ccgcgaaggc gccctcggcg tcggtgtagg tggcggcccg caccccgcgc
    51121 acccgcgggt cggcgccgcg gaccatgcgc tcgagctcga tggccatggc caccttgtcc
    51181 tccaccggcg tggcgagcac ggcctcgcgg tgcacgtcga ggctggtggc ggcgacgccg
    51241 tcgggctcgg ccaggccggc ccactcgtcg ggctcggcga agggcaggtt gtcccgggcc
    51301 tcggccaggg cgtcggcgac gatgtcgtcg tcgtggctgc cggcgtaggc gaagccggtg
    51361 cggccgtcct tgatgacccg gatgccgatg ccggcggagc cggcctgggt cagggattcg
    51421 atctcgccct cgtaggcgtc gacggaggtg ttggtgccct ggctgacgta ggcctcgacc
    51481 tgctcgcccg ggccggcctg ggacgcgacg cggtcggcca gcgcccggag ggcgtcgacg
    51541 tcggtgcccg tgcggggttc ggccggcgcg ctcacgagct ggtgccgccg atggtgagct
    51601 gggcgacccg cagcgtcggg cagccgtcgc cgaccgggac gccctggcca tccttgccgc
    51661 acgtgccggg gccgccgaac gcgaagtcgt cagcgatgac ctcgatgtcc tggaggcact
    51721 gcggaccgtt gccgatgagg ttgccctcgc ggatcggctc ggtgatctcg ccgtcctcga
    51781 tgaggtacgc ctcctgcatg ccgaagacga agtcgcccga ggcggtgttc acctggccgc
    51841 cgccgagctt ggccacgtag acgccgtggt cggtgccggc gatgacgtcg tcggggctgg
    51901 cggtgccgtt ctcgaggaac gtgttggtca tgcgcaccat cggcaggtgc ttgtaggact
    51961 ggcggcggcc gttgcccgag ttcgcccggc cctccttgcg gctgcggagg aagtcccaca
    52021 tgtactcggt gagcacgccg tcctggatca acacgttgcg ctgccccggc gagccctcgt
    52081 cgtcgaccgc gaaggcgccc cactcctcgg cgatggtgcc gtcgtcgacc agcgtcacga
    52141 gcgggctggc gacgagctcg ccgatgcgcc cggcgtacac cgaggcgccc ttgtcgacca
    52201 ggtccttctc gaggccgtgc ccgcacgcct cgtggaagag cacgccgccg gatccggcgc
    52261 cgatcaccac cggcagctga ccggacgggg cgggccgggc ccgcagcttg gtgatggcca
    52321 tctgggcggc ctcggcggcg aactcgtcga tgtcgatgcg gtcgaacagc tcccagccga
    52381 tggtgaggcc ggtggacttg cgcccggtct gcatgccggt gtcgccagtg gccacggcac
    52441 tgaccgagaa cagggtgcgg acctggtcgt cgctggtgag cagcccgtcg ctgttggcca
    52501 cgaggatgcg gcggcgggag ccggccaccg acgcggtgac ctgggagatc tcgcgggcgg
    52561 cagcccgggc ggcctcgtcg gcctggcgca ggatggcgac cttgtcggcc ttgggcacgt
    52621 cgtcgggcag cacgctgacc gggctcgggt tggcgacgtc gcgccgggtg agggcgatct
    52681 cgcgcacgcc gccgccgccc tcgcgggctg cggcggatgc ggcctcagcc gcggtgagca
    52741 ggccctgctc gctgaggtcg gcggtgtggg cgaagccggt ggtgtcgccc accacgaccc
    52801 ggatgccggc gcctcggtcg cggccggagc tgagctgctc cacccggccg tcgtccagcg
    52861 tggcgttgcc gctctgcttg tcctcggcga acacctcggc gaactcgccg ccggtgcgca
    52921 gggctcgggc cagcactcgc tcgagcgtgg gatcctcgat cacttcgtcc tccggggtcg
    52981 cgggacgggc ctggggggcc gatgtgactc gggccgaccc gaacgacgta gcgtaccgat
    53041 gtgcccctcc agaccggtct ctgcgccacg gtcgagatcg tcgtcaccga cgacgacacc
    53101 gccatcgcca tcggctccgg ctcggtcccg gtgctcgcca ccccccgcct cgtcgccctg
    53161 tgcgagcagg ccgccgtcgc ggccatcgaa ggcgagctga ccggcggctc cacgtccgtc
    53221 ggcatgcggg tccaggtcga ccacctcatg cccaccgccg tcggcgcccc ggtcacggcc
    53281 gaggccacgc tcgagaaggt ctccgggcgc cggctgacct tcacggtgtc ggcatcggac
    53341 caccgcggcc tgatcgccgc cggccgggtc acccgcgtcg tggtcgacta cgaccgcttc
    53401 atggagaagg ccaactagtt ctggcagcgg ccacggaacg ggccgctgac gttcagacgc
    53461 cggcgaggaa gtcgagcatc accgcgcgcc acgcgtcggg gttctcgaac tggggcgagt
    53521 ggccggcgtc ggcgatgacc tcgagccggg cacccgcgac ggcagtggcc atggcctcgg
    53581 acgggtcgag gaacggggcg tcctgctcgc cgacgatcac gagggtgggg accgagaggt
    53641 cggcgaggtg ctgcagccgg tcgagctgat cgatcatctg gccgatcatc gaggcgtaca
    53701 tggccggcga gctgcccagg aacttctggt cgccgagctc gcggtagccc gggcgctcgt
    53761 cgaggaggcg ctggttggcg ggcgtcccga gcggtccgtc gccgcccatg gcgtccatgg
    53821 cctccttcac cgcgaccatc ccgccctcgc gcacgatctc ggcgccgagc atggccagct
    53881 cacggtcgat ctccagcggt ccgtgcgtcg tgtccatgag gacgaggccg tcgagcgcgt
    53941 ccggccgctt cgccgcggcg acctgggcga tcatcccgcc catcgagtgg ccgagcagga
    54001 cgaaccgctc ccagcccagc gcgtcgacga gggacagcat gtcgccggcg aagtggtcga
    54061 aggagtactc ggactcgtcc tcggggtgcc aggagtcgcc gtggccccgc aggtcgggcg
    54121 cgaccaccca ccagccggca tccgccaggg cctccatgtg gtcggcgaag tcgatccggc
    54181 cgccggtgaa gccgtgcacc agcagcaggc cccgtccgcc gacgccgacc tcgtcgatgg
    54241 tcaggtgctc cccgtcgctg atgtcgatca cccgtgtcgc catccgcaca tggtggctca
    54301 ccgctcgtgc cgtcgccgtc accactgcag gtggtcgggc agctcgcgca cgtcgaccgc
    54361 cccagccggc acgtcgacgg tcgttcccgg cggcagggcg cggcgccgga ggaccacggg
    54421 acgctcgacc ccggcgacgg tcaggcggac ctcgccctcg ccgaccgcgt cgacggcccg
    54481 cacctcgcga cccgccacca cgtcgccggg gccgatctcg tcgggctgcg gcggaggctg
    54541 gcgggcgtcc aggaccacgt cgggcagcgc acctcccgcc tcggcgatga cgtcgaggtc
    54601 gaagcggatc cactcgacgt cggcgatggt ccgccgggcc tcggggtgca gccaccacca
    54661 gtgctcgtcc gcggtgagga cgtgctggtc gacgatgccg gccggggtgt cgaggacgaa
    54721 gccgacggtg ccgtcaccgt cgtcggtcca ggtggcctcg accacggcac gaccggagcc
    54781 atccggcagc gggccgaagt cggccacgag ggcgacctcg tggggaccgt ccgcctccgg
    54841 ggccggcggc gccgagcccg ccggatcgga gctcggatcg acagcagccg cgggggcggg
    54901 ggacgagggg gacgcgccgc tgctcgagca ggcgctcagc acgagcgcgg cgacgagcgc
    54961 gatgcagccg aggtggcggg gacggacggg cacgatggac ctcccggatc gaccggccat
    55021 tgacagccgg gatgcaacgg gtggcgtggg tcgaccaccg aaccgtgacc gaggctagcc
    55081 acccccggcc ggctgcgtgg gtggatcgtt cgggcgcccg gcctcgccgt agtctggcgg
    55141 ggtgcgggga cagaggcaga cgggcgcatg accgccggcg ccgtggacca ggacgatgcc
    55201 accaccgcga ccgacgcgca ggtcgccgcc agccggctgc gctggtggcg cgagctcctc
    55261 tacgtcgccg tcttctacgc cgtgtactcc tcggtgcgga acctgttcgg ctcccagggc
    55321 acgggcgagg acatcgacgc ctccgtcgcg ttcgagcacg cccgccagat catcgacatc
    55381 cagcgcgcca tgggcctcta cttcgaagcc gagctccagc gctggtacct cgacctcccg
    55441 gcgatgggcc tgatccgggt ctggaacatc ttctacggca cagcgcactt cgtggtgacc
    55501 gccggcgcgc tcgtctggct gtaccgcagc gacaaggccc gctacccccg ctggcgcaac
    55561 acgctgctgg tcacgacggc ggtggccatc gtcggcttcg ccagctactc actgatgccg
    55621 ccccggctgc tggacgaccc cggcaagtac ggggcgtgcc gcatctacgc cccggaggcc
    55681 gcgttcaccg ccgagccggg cgacaagtcg gtcgagggct gcgaccggta cgggttcgtg
    55741 gacaccctcg ccgtgcacgg cgggtggatc tccttcgacg acgagaaggc cgccggcctc
    55801 accaaccagt acgcggcgat gccgtcgatg cacatcgggt ggtcggtgtg gtccgccctc
    55861 gtgctggtgc cgatgatgcg gcgacggtgg acccgggcgc tggcctggag ctacccgttc
    55921 ctcaccctgt tcacgatcat ggtcaccgcc aaccactact ggatcgacgc ggtgggcggc
    55981 ctcgtcgcgc tgggcttcgg cgccctcgtg gcccggtggc tcaccgcccg cttccagtcc
    56041 cgccaggctc gaggcgacct cccgatcacc accgcatagg cgttccgggc cgcggcacac
    56101 gcctcggagg cgtgtgggac gtcccggtcc ctcaggtgcc gcccagccag gtgaccggcg
    56161 gcccgtggta cccggtgctg gcgagcagct cctcgtcgtg gccgccggcc cgccccggcc
    56221 aggacgacat ccgcacgtcg gcgacgtcgc cggccggttc catgcgcagc cagtggatcg
    56281 gcgcatccgg ggtggcgcgc gcaccggcct cctcgatggc ggcgcgcagg cggacgaacg
    56341 acgaccgcgg caggtactgc agcacgatcg agtggtgcac gacggtggcc aggcccggcg
    56401 tgggctcggc cagctgcgcc tcgatccagt cgccggcatc catcgcatcg acctccaccg
    56461 ggtggtgacg ggcgacctcg atggccgcgt caagcctggc ccggcgctcg acctgatcgg
    56521 gccacaggaa cgagcggagg cgggtggcgc cctcgtccgt ggttggatcg atcggggcct
    56581 ggtcgcagcc tcgccgctcc gccacgacca ggtcggtggc caccggcggc aggtcggcgg
    56641 tccacgggtc ctcgaacacc aggccgtcgt cgggacccca ggcgagcgcc ccgccgccgg
    56701 cccggtaccc gtagcgatcc cagtgcagga gcaggccggc gctggcaccg acctcgagct
    56761 ggcgcagcgg caggccggtg cggcgggcga cggcgtggaa gccaccgaag agcgacgtgg
    56821 cccgcccgac ctcgttggtc tgcacgccca ggtccatggc cgcctcgatg cggtgccggt
    56881 gggcctcgac ggtggcgagg aaggcgggga ccgggtcgtc ggtgtgggag ccgccggccg
    56941 aggcgtagtg ggcggcgagc tcgggggcgt caccggtgag gacgagggcg tgcacggcgg
    57001 cgaggaagcg cagggggatg gcgtcggcca tggcgttccc ggcccacggc cgcaggatct
    57061 gggtgcaggg cccgtcggtc cgcacgtcgt cggccatggc gtcgaggatc cgcgcgtaca
    57121 gggccgatcc ggcccgttcg caggcggtgc gctgcagcgc gaggacgtcg atcaggggca
    57181 ggtcacggct gcgctccacc agggaccctg ctcgatcagg cggcgcggcg gacggggcag
    57241 accagcaccg agccctcgac acccttgaag gcgcgcctgc gggcgcggcc gaactcgacg
    57301 ccgggcggcg ggctgccgat ggcggaccgc acgtcggtgg tggccagcac ctggccgccc
    57361 ttggccgagt ccgccacccg ggcggcgagg ttcacgtcgt ggccgatgac gtcgtcgccg
    57421 agcagctcga cctcgccgtg gtgcagcccc acgcgcaggc gcagaccggt ctcgttggtc
    57481 tcgttgatct cgagggcggc gagcacggcg gcctcgggcg ctccgaagga gatgagcagc
    57541 ccgtcgccga gcttcttcac gatgcgcccg ccacggctgc ggatgacagg accgacggcc
    57601 cggtggtgca gctccagcag ctcacgggcc gcccgatcgc cctcgcggga cgtgaagctg
    57661 gtgaagccct cgaggtcggt gaaggcgatg gtggcgacct ggcgggcgtc gccggtccgg
    57721 ccgtcgtcgg tgtcgacgtt gagcagctgg atggcgttca ggcccatgtt ggcgatgagc
    57781 gagggctcct gctcgatcga gcgctcgagg aaccgctgga tgacgtcgag cgacgtggcg
    57841 gtgcggatgg gccgctcggt gggctgctcc agccaggccc ggtcgatgag gccgacctcg
    57901 gacgctccgc gggcgaagtc cgggtgctcg ttcaagaggt cggcggcctg ctgggtcagg
    57961 cggcgccgga tcgagtcgat ggcgctctcc ccacgggccc gggcaccttc gagcgacggc
    58021 atggcggcga atctagacgg cacctccccg gtggccgacg tggtcgatcc ggaccgggca
    58081 cgggtaccgt attcaccgca tgctgctcga ccggatcaac agccccgccg acctgcgagg
    58141 cctcagcgcc gaccagctcg agacgctggc tgcggagatc cggaccttcg tcgtcgacgc
    58201 cgtcgatcgc acgggcgggc acctcggctc caacctcggc gcggtggagc tcaccctcgc
    58261 cgtgcaccgg gtgttcgagt cccctcgcga catcgtgctg tgggacaccg gccaccaggc
    58321 gtacgtccac aagatcgtca ccggtcgtcg ggacgggttc gccaacctgc gccaggaggg
    58381 cggcctgtcg ggctacccgt cgcgcaagga gtccccgcac gactgggtcg agaacagcca
    58441 cgcctccacc atcatcagct acgcccacgg cctcgccacc gcgatgtcgc ggaacgagat
    58501 caccgaccgg cgcatcgtgg ccgtcatcgg cgacggctcc ctcaccggcg gcatggccta
    58561 cgagggcctg aacaacctcg gccacgccgg ctcccgggcc atcatcatcc tcaacgacaa
    58621 cgggcgctcc tacgccccca ccgtcagccg cctgggcgac agcctcgctc gcctccgcat
    58681 cgatccccgc tacgtgcgca accgggcccg gttcgagaag ctgctccgca acgtgccccg
    58741 cgtcggtgag atgctcgagc gcggcttcga tgcgacgctc gccggtgtcc gcgagtactt
    58801 cgaaccgccg gcgttcttcg aggacctggg catccgctac accggcccgt tcgacggcca
    58861 cgacatcgag ggcctcgagt ccgccctgca cgacgccgcc gagtacgacg gaccgatcgt
    58921 ggtccacgtc ctcacccaga agggccgcgg ctacgcgccg gccgagaacg acccgatcaa
    58981 gaacatgcac gacaccggat ccgtcgagcc cgggtcctgc gccgcagcgt tcaccgaggc
    59041 gctcatcaag gaggccgagc agcggcccga ggtcgtggcc atcaccgcag cgatgcccga
    59101 ctccaccggc ctgctcccct tcgccgagcg cttccccgac cgcctgatcg acgtcggcat
    59161 cgccgagcag cacgccgtga cctccgccgc cggcatggcc atgggcgggc tgcgcccggt
    59221 cttcgccgtc tactcgacgt tcctctcccg ggccttcgac caggtgaacc tcgacgtcgg
    59281 cctccacgac cagccggtcg tcttctgcct cgaccgggcc ggcatcaccg gggccgacgg
    59341 gccgtcgcac cacggcgtgc tcgacctcgt cctgttcacg aagatcccga acatgaccgt
    59401 gctcgccccc tcctcgtacc aggaggtggg cgtgatgctc cacgacgcgc tcgaccagag
    59461 cgacggcccg gtgctgatcc gctggcccaa ggtcctgccc gagtgcggcc ccgacgaggt
    59521 cggctccgga ctgaagggcc gcaaggtccg tcacggccgc gacgtgtgca tcctggcggt
    59581 gggcaagatg ctgcacccgg cggtggaggc cgccgaggcg ctcgccgccg acgggatcga
    59641 ggccaccgtc tgggacgtgc gctgcgtgaa gcccgccgac cgcgagatgc tcgacgacgc
    59701 cgccgggcac cctgccgtgg tcaccgtcga ggacggctac aaggccggcg gcgccgggtc
    59761 cctgctcgcc gccggggtcc gggagtgctg cgaggagcgg ctcgttcccc gcatcgagat
    59821 cctcggcgtg cccaccgagt acctagacca cgccaaggtc gacgcgatca tgggccgcct
    59881 ctgcctcgac gccgacggca tccgctcctc ggtcctgcgg gccgtgcggg ccc
//
LOCUS       DATISU010000281         3865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_529837, whole genome shotgun sequence.
ACCESSION   DATISU010000281 DATISU010000000
VERSION     DATISU010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529837"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VLR27_11200"
     CDS             <1..514
                     /locus_tag="VLR27_11200"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HSP04062.1"
                     /translation="PLVSEATELARQRQLIDEVAEAVFEEAGRRVEYQVGTMIEVPRA
                     ALTSEDIAEHADFISFGTNDLTQTTFAISRDDAEQAFLLQYLADKILTENPFASIDAK
                     GVGRLMGIALDGARETKPAIETGVCGEHGGDPASIALCHEMGLTYVSCSAYRIPVARL
                     AAAQAALAAG"
     gene            complement(581..1312)
                     /locus_tag="VLR27_11205"
     CDS             complement(581..1312)
                     /locus_tag="VLR27_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSP04063.1"
                     /translation="MTPTITEVADDIYRVSTWIPDVTPEGFTFNQFLVVADEPLLFHT
                     GHRGFFPLVSGAVAEVLPVESVRWISFGHVEADESGSVNTWLASAPDSEVVHTPLACD
                     ISLNDLCDRAPRALAEGEVLDLGGKRVRSIATPHVPHGWEAHVLYEETTGTLLCGDLF
                     SQIGAPAPLVSTDIVGPAMAAEAMFGATSLAPHTGATIRSLGDLEPRTLAVMHGSSFE
                     GDGRQALRDLAAAYDELIAGSPVPA"
     gene            complement(1335..3290)
                     /locus_tag="VLR27_11210"
     CDS             complement(1335..3290)
                     /locus_tag="VLR27_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04064.1"
                     /translation="MTTVADAERSLELAAEARAAFDIDGVVAHLSSAMRVLTAVGDVR
                     RAAMVCVALGDALANGMGNLTAGRAWFARANAMLADEPPCLEQGWAAVAAMGCDVGDP
                     DELLACAELALDRARRFGDVNLETKALADGGLAHVQAGRVEQGMAMLDEAMALVCGPA
                     DDEDAAGKSACSFFTACYYSADFERAASWADLLRAKGLIGAAPVTPIFLSSHCATVQA
                     CLLCELGRWGEAEALLEQASEEFTAITGMPGFHPVVLLADLRIRQGRLHEAEVMLLGK
                     GQAFEALLPLARLHLARGDHRLAAAAARRGLRSLGPDHLRAIELLAVLVDAELAEGDA
                     AAAAAACSELSGRLTVVGNPVLSARAAGARARVLAAAGDLAGAVELLEGVLGELPAGR
                     APYQQARLHLELARHRSAAGDGSGAQLDARAAADVLDELDVVIAPDDRRLLDALVATP
                     APEPVGFALVDHGGWYELVAGETRVRVPSTKGLRYLAELVRGPGTERHVLDLVDRVEG
                     VAAGGEIDRRAIGDAGEVLDHTARDAYRRRIEQLRAECDDLLDAGDLDGAEARQAELD
                     QLVAQLAAAFGIGGRERRASSTVEKARLNVTRALRTAIRRIEEVIPEGGVLLDRAVRT
                     GTYCTYEPGAGEPRWIVQPGVNGTAPV"
     gene            3328..>3865
                     /locus_tag="VLR27_11215"
     CDS             3328..>3865
                     /locus_tag="VLR27_11215"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HSP04065.1"
                     /translation="MIDTAPQTPGFETIEVAVDGATGSLWLARPDKLNPLSTQTLREL
                     EDAARWFDEHPDLKVVTVGGRGRAFSAGADLGAFTGEGGRGDREQGWRTARALEEMRA
                     VTVARIQGWCVGGGLVIAAACDLRIARASASFSIPEIDLGIPLAWGGIPRLVREIGPA
                     RTKELVMTGREFSPEEALT"
BASE COUNT          532 a         1420 c         1420 g          493 t
ORIGIN      
        1 cccgctcgtg agcgaggcca ccgagctcgc ccgccagcgc cagctcatcg acgaggtggc
       61 cgaggccgtc ttcgaggagg ccgggcgccg ggtcgagtac caggtgggca cgatgatcga
      121 ggtgccccgc gccgcgctca ccagcgagga catcgccgag cacgccgact tcatctcctt
      181 cggcaccaac gacctgaccc agacgacgtt cgccatcagc cgcgacgacg ccgagcaggc
      241 gttcctgctc cagtacctcg ccgacaagat cctcacggag aaccccttcg ccagcatcga
      301 cgcgaagggc gtcggccggc tcatgggcat cgcactcgac ggggcgcgtg agacgaagcc
      361 ggccatcgag accggcgtgt gcggcgagca cggcggtgat ccggcctcga tcgccctgtg
      421 ccacgagatg ggcctgacct acgtcagctg ctcggcctac cgcatcccgg tcgcccgcct
      481 ggccgcggcc caggccgcgc tggcggcggg ctgacctcac acgagcgcgc cgatcggcgc
      541 gctcgtgtcg ctggggagca cgaacgcgcc gatcctgggc tcaggcgggc accggcgatc
      601 ctgcgatcag ctcgtcgtag gccgcagcga ggtcccggag ggcctggcgg ccgtcgccct
      661 cgaacgagga cccgtgcatc acggcgagcg tgcgcggctc gaggtcgccg agcgagcgga
      721 tggtggcgcc ggtgtggggc gccaggctgg tcgccccgaa catcgcctcg gcggccatcg
      781 ccgggccgac gatgtcggtg ctcacgagag gggccggcgc tccgatctgg ctgaacaggt
      841 ccccgcacag cagcgtgccg gtggtctcct cgtagaggac gtgggcctcc cagccatggg
      901 ggacgtgcgg cgtggcgatc gagcggacgc gcttgccgcc gaggtcgagc acctcgccct
      961 cggccagcgc ccgcggggcc cggtcgcaga ggtcgttgag cgagatgtcg caggcgaggg
     1021 gcgtgtggac cacctccgag tcgggtgccg aggccagcca ggtgttcacc gaccccgact
     1081 cgtcggcctc gacgtggccg aagctgatcc agcgcacgga ctccaccggc agcacctcgg
     1141 cgacggcgcc ggacaccagg gggaagaagc ctcgatggcc ggtgtggaac agcagcggct
     1201 cgtcggcgac gacgaggaac tggttgaagg tgaacccctc gggtgtgacg tcggggatcc
     1261 aggtcgagac ccggtagatg tcgtcggcga cctcggtgat ggttggtgtc atgggttcca
     1321 tgacctgatg gcgttcagac cggggcggtc ccgttcactc cgggctgaac gatccacctc
     1381 ggctcgccgg cgcccggctc gtaggtgcag tacgtgccgg tgcgcacggc acggtcgagc
     1441 aggaccccgc cctcggggat gacctcctcg atgcgccgga tggcggtccg cagcgcccga
     1501 gtcacgttga gccgcgcctt ctcgaccgtg gacgacgccc ggcgctcgcg gccgccgatt
     1561 ccgaacgctg ccgcgagctg cgccacgagc tggtccagct ctgcctggcg cgcctcggcg
     1621 ccgtcgaggt cgccggcgtc gaggaggtcg tcgcactcgg ctcgaagctg ttcgatccga
     1681 cggcggtagg cgtcgcgggc ggtgtggtcg agcacctcgc cggcgtcgcc gatcgcccgg
     1741 cgatcgatct cgccgcccgc ggcgaccccc tccacccgat cgacgaggtc gagcacgtgg
     1801 cgttcggtgc ccgggccgcg caccagctcg gccaggtacc gcaacccctt cgtcgaaggc
     1861 actcgcaccc gcgtctcgcc ggcgaccagc tcgtaccaac cgccgtggtc gacgagggcg
     1921 aagccgacgg gctcgggtgc gggcgtcgcc accagcgcgt ccagcagccg gcgatcgtcc
     1981 ggggcgatga ccacgtcgag ctcgtccagc acgtcggctg ccgctcgggc gtcgagttgg
     2041 gcaccggagc cgtcgccggc cgccgagcgg tggcgggcca gctcgaggtg gaggcgggcc
     2101 tgctggtacg gcgcgcggcc ggccgggagc tcgcccagga ccccttcgag cagctcgacc
     2161 gcaccggcca ggtcgcctgc ggcggcgagc acacgtgccc gcgcgcctgc tgctcgtgcg
     2221 ctgagcaccg ggttcccgac caccgtcagg cgtcccgaca gctccgagca cgccgcggcc
     2281 gccgccgctg cgtcgccctc ggccagctcg gcgtcgacga ggacagcgag cagctcgatc
     2341 gcccgcaggt ggtcgggtcc gagcgaccgg agaccccgac gggcggcggc cgcggccagc
     2401 cggtggtcgc cgcgagcgag gtggagccgg gcgagcggca gcagcgcctc gaacgcctgg
     2461 cccttgccga gcaacatgac ctcggcttcg tgcaaccgcc cctggcggat gcgcagatcg
     2521 gcgagcagga ccaccggatg gaagccgggc atgccggtga tggccgtgaa ctcctccgat
     2581 gcctgctcga gcagcgcctc ggcctcgccc caacggccga gctcgcagag caggcacgcc
     2641 tgcacggtcg cgcagtggct gctgaggaag atcggggtga ccggcgccgc gccgatgagg
     2701 cccttggccc ggagcaggtc ggcccacgag gcggcccgtt cgaagtcggc gctgtagtag
     2761 caggcggtga agaacgagca cgccgacttg cccgccgcgt cctcgtcgtc ggccggcccg
     2821 cacaccagcg ccatcgcctc gtcgagcatc gccatgccct gctcgacgcg accggcctgc
     2881 acgtgggcga gccccccgtc ggccagggcc ttggtctcga ggttcacatc cccgaaccgc
     2941 cgggcccggt cgagcgccag ctcggcgcag gccaggagct cgtcgggatc gccgacatcg
     3001 caccccatgg cggcgaccgc ggcccagcct tgctccaggc acggtggttc gtcggcgagc
     3061 atggcgttgg cgcgagcgaa ccacgcccgg ccggcggtga ggttgcccat gccgttggcc
     3121 agcgcgtcgc cgagcgccac gcacaccatc gctgcccgac ggacgtcgcc gacggcggtc
     3181 agcacccgca tcgccgacga caggtgggcg acgacgccgt cgatgtcgaa cgcggcgcgc
     3241 gcctcggcgg ccagctccag tgagcgctcg gcgtccgcca ccgtggtcac gcccccagcc
     3301 tcgcaggtgc gggcctacgg tgcttccatg atcgacaccg ccccgcagac acccgggttc
     3361 gagaccatcg aggtcgccgt cgacggcgcc accggatcgc tctggctcgc ccgtcccgac
     3421 aagctgaacc cgctgtccac ccagaccctg cgcgagctgg aggacgccgc ccgctggttc
     3481 gacgagcacc ccgacctcaa ggtggtcacg gtgggcggac ggggccgggc gttcagcgcc
     3541 ggcgccgacc tgggcgcgtt caccggcgaa ggtggccggg gcgaccggga gcaggggtgg
     3601 cgcacggccc gggcgctcga ggagatgcgc gcggtcaccg tggcccgcat ccagggctgg
     3661 tgcgtcggcg gggggctcgt gatcgcggcg gcgtgcgacc tgcgcatcgc cagggcgtcg
     3721 gcgagcttct cgatcccgga gatcgacctc ggcatcccgc tggcctgggg cggcatcccc
     3781 cgcctggtcc gcgagatcgg cccggcccgc accaaggagc tggtgatgac cggccgggag
     3841 ttctcgccgg aggaggccct gaccg
//
LOCUS       DATISU010000282        19476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_669387, whole genome shotgun sequence.
ACCESSION   DATISU010000282 DATISU010000000
VERSION     DATISU010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 19476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19476
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669387"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2193
                     /gene="mbhE"
                     /locus_tag="VLR27_11220"
     CDS             <1..2193
                     /gene="mbhE"
                     /locus_tag="VLR27_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HSP04066.1"
                     /translation="ASFTWVPGLDLSVDLFVDGLGLVMILLVSGIGLAIAAYSGSYFA
                     PNATAARTAGLLVLFAGAMLGLVTAEHVVILYLFWELTSILSWLLISQRHTDPQARAA
                     ATHALVLTGSGGLALLGGLVILAREAGTWSVVELLADPPGGTAAGVGLGLVVLGIVTK
                     SAQYPFHAWLPGAMVAPTPVSAYLHSATMVKAGIYLALRFAPSFADLDAWQPVVLTIG
                     LLTMVAGGLRAIRQDDLKLLLAFGTVSQLGFLFVLTGLGFEDATIAGVTLLVAHALFK
                     ATLFLVVGIVDHQAHTRDRHELLDLRLGNGWGPVRVCAVLTAASMAGVPLLAGFIAKE
                     EAYAAFVYEGGTLGVIGLVGIVTGSILTMAYSLRFASVFWRQSDRDDADVNPPSWTFG
                     APVYVLTALTLVIGLVPAPIIGDLVDVAAGDLLGVEVASKLALWHGINTALILSLVTI
                     AAGVLLWRLTDPFVRLTGRAAPPIDGYRAFQIGLEGLFTVARVTTTRIQTGSLPRYVA
                     IILMVVVGGPALVAVATTSMPDLADPTDVPVQLALAGFIAGAALATIFVPRRLGAVVV
                     LGGVGYGMAGLFVAQGAPDLALTQFAIETLTIVAFVLVLRQLPARFGTDGDRAGKARR
                     LGAAAVVGLGAFVLVLAVSGTDPTTPASDLAIEQAVPEAEGRNIVNVILVDFRGLDTL
                     GEIAVLLVAAVGVTTLARVGRRPPRTGVPPLPEIDPLDPTTMDKDART"
     gene            2190..2675
                     /locus_tag="VLR27_11225"
     CDS             2190..2675
                     /locus_tag="VLR27_11225"
                     /inference="COORDINATES: protein motif:HMM:NF015970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HSP04067.1"
                     /translation="MKRSVIVDVTARLILPSALLFALHLLLSGHDRPGGGFVGGLVAG
                     ATIALIFVAGGMDDVRSVVPVRPWTILGGGLAIAACVATVPILLGNPVMDNWKFYVDV
                     PLLGEAKISSALVFDIGVAAVVIGTIFMVFEAFGDEEPPEFRGEVREGPGSGPRREVG
                     S"
     gene            2672..3082
                     /locus_tag="VLR27_11230"
     CDS             2672..3082
                     /locus_tag="VLR27_11230"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HSP04068.1"
                     /translation="MTTVFAALAGALFATGTYLVLQRELTRMLIGLALLSHGANVVLL
                     VAAGDPGRPAFLDGRDEPSAFLDPLPQAFALTAIVITFGVTCFLLALAYRSWVLTQDD
                     TVHDDPEDVRVREEPDADIERSGVDQPTIEAQDR"
     gene            3079..4542
                     /locus_tag="VLR27_11235"
     CDS             3079..4542
                     /locus_tag="VLR27_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSP04069.1"
                     /translation="MTPWIALPAVLPLLAAAASVLVGRSRTAQRIIGLGTLTALLGLA
                     VRLLIETDRNDEMTTQAGGWVAPLGITFVADRLSAIMLLVAIGMFLAVLLYAIGQGDE
                     ESRHVGFHPVYLTLVAGVCMAFLTGDLFNLFVAFEVLLVSSYVLLTLGGRLAQVRAGT
                     TYVVISLLASITFLMSLGLLYRATGTVNLADLPAAMEVLDPELRRVFLLLFLGVFGIK
                     AAIFPLFFWLPDSYPNAPTAITAVFAGLLTKVGVYAMIRIQTTIGPDDTVVGTVLLVV
                     AALTMVIGVFGAISQQEIKRILSFHAVSQIGYMIFGLGLLTVAGLAAAILYITQYIVV
                     KTTLFLTGGLVERASGTGHLDRVDGMLRTRPLLGMLFLLPALSLAGVPPLSGFVGKFA
                     LVDAGVSSRSWILVGVSLAVSIMTLLSMNKIWAGAFMGEPTANAEATHPLPTAMVFAT
                     GVLVVVSIGIALGAGPIYAFAERAAETLLPVAGSAAP"
     gene            4539..5051
                     /locus_tag="VLR27_11240"
     CDS             4539..5051
                     /locus_tag="VLR27_11240"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HSP04070.1"
                     /translation="MSYVGPVIWLTGLWMILWRSTTPGTIVTGIVLAVALTWVVRRSD
                     VHRRHHRVHLLPLLRFFGHMAVALVKSNIALGFEVLTPTDYTTPGLLEVRLPTCSELV
                     LTVIANSITLTPGTMTLELRPDTSTLLVHVLHLRNVDAARDEILELHRLVSAALVPVA
                     TPDERGAEIT"
     gene            5048..5299
                     /locus_tag="VLR27_11245"
     CDS             5048..5299
                     /locus_tag="VLR27_11245"
                     /inference="COORDINATES: protein motif:HMM:NF015993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HSP04071.1"
                     /translation="MIVATFVLLSCAAALFAYRAVRGPSIVDRVVAVDGLVIIAIGAI
                     AADAVRYDDARFADAAILLAIIAFVGTGIVARFIERRGG"
     gene            5302..5658
                     /gene="mnhG"
                     /locus_tag="VLR27_11250"
     CDS             5302..5658
                     /gene="mnhG"
                     /locus_tag="VLR27_11250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HSP04072.1"
                     /translation="MDLVADLIMLAGALWFLLAGLGVARLGDSLARVHAATKATTLGM
                     MLVVLGAALQLPLDDGLRIALAIFLIALTNPLGGHLLGRAVEANPGTAEMRIDVRATL
                     DDDGGDDDGSLEPDQG"
     gene            complement(5665..6426)
                     /locus_tag="VLR27_11255"
     CDS             complement(5665..6426)
                     /locus_tag="VLR27_11255"
                     /inference="COORDINATES: protein motif:HMM:NF020604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HSP04073.1"
                     /translation="MSAERRERGTTWPPKRTIVLCATPRTGSTLACDVLTGTGLLGFP
                     KEPFAEVAIPACADAWAVPTLDEDPHRYVRAAFTNATTPNGICAVKLMWDDVSRLARA
                     GDRHEADVFDCFVDPVALLVTRRDRLAAAISQHRAEQTGEWSTAATEHRPMPGPPDLD
                     RVSRLHDAQHEGADRWRALVLTTAIPWAEVVYEDVARDPSCIASVAAGLIGVELPERP
                     DVATRLRVQRDDWNDEVRRAWVHATGGCDRGCPAA"
     gene            complement(6423..7415)
                     /locus_tag="VLR27_11260"
     CDS             complement(6423..7415)
                     /locus_tag="VLR27_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04074.1"
                     /translation="MPRRHVYTRHIGWPGDGVARIATPDATYRASDLVDADWVRANRD
                     EFDCMHTHFGFGDVDLPSMRAWLAALVEVGRPLVHTVHDLENPHRADHRHHRRQLDLL
                     VPAADALITLTGCAADRVERDWGRRPVVLPHPHIVELERVGEPRVRTAPLVVGMHLKD
                     LRAAVHGPELWEVVADVVGGDERFRLRVDAYPAAVERQPDAGRRLEQLAAAPDVELQL
                     RPYGPDDEFEAYVRSIDVALLPYRWGTHSGWAEACLDVGTQVVAPTSTCIPDQHPSIH
                     ALDLDAPDVGTALARVLDACWAHRDEPALSREWRTQQRQDLAAAHAQIYEAVTS"
     gene            complement(7427..8482)
                     /locus_tag="VLR27_11265"
     CDS             complement(7427..8482)
                     /locus_tag="VLR27_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04075.1"
                     /translation="MTDPRPFAFYAHHQGRGHLSRIAAVCEALGPERCTVATSHPDAR
                     HALPRGADVCPLPTDVPPGPAGDVTAGGALHWAPEHEVGAARTHAITGWLLDRRPSVA
                     LIDVSVEVALTVRLAGVPVVVVRLHGDRADAPHDLAHRLARSIVAPFPAELEHPSTPA
                     WVRVKTVHSGFVTPATRFTGPDRDPGGQGRVLVVWGQGHPPPPAAALDAAAAATPGRT
                     WEMVGPPCPDGVPEHVRHHGWVDDVAACIATGDVVIGPPGDGLVGDVAGAGARFVAVC
                     EPRPFDEHRYKAEALEAAGAAVVLDRWPEPGAWRAVLQHADGLDPGVLASLGTDGAAR
                     IAAHLGDVAAGHRGRVA"
     gene            complement(8479..9564)
                     /locus_tag="VLR27_11270"
     CDS             complement(8479..9564)
                     /locus_tag="VLR27_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSP04076.1"
                     /translation="MSELPLRIGLIGSVRFPQRPPFAGGLEAHTWMLAEGLRDRGHEV
                     TLFGTDAPEGCTVRLVTADWAPSEVARADVSSVPAEVVSEHHAYLRILRSLALGEVEL
                     DVVHNNSTHLLPLAMAALVPVPMVTTLHTPPTPWLESALAAAGPKTGHVVSVSLANAA
                     QWAPGTVDEVIGNGVDCDLWRPRLGVRPEGAAWFGRLVPEKAPHLAIDAARAAGLTID
                     LAGPMHDRCYFEREIQPRLGDEVRYLGHLSPEGCAELVAGAEVCVVTPVWAEPFGFVV
                     AEALACGTPVAGFAAGAVPELVDDAVGRLAPVGDVAGLAAAILEARDLDRDACRRHAI
                     ERFSLDTMVDRYAAAFRRCTSDPCARP"
     gene            complement(9561..10433)
                     /locus_tag="VLR27_11275"
     CDS             complement(9561..10433)
                     /locus_tag="VLR27_11275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSP04077.1"
                     /translation="MVTLVRNRNALLRSFLAGVAAQDAPVEVVVVRAGGDEDPREVVA
                     ELDGLRATVCEVGAPDDRIPYSEARNAGAAAASGDHLAFCDADTIASPTFAGAIDAAL
                     ARHDALCTGEVRYLPPGDVSGLDFPTLLRRSRPHPHRETVPDHGVVLSDRHELVWGLC
                     MAMRHSTFESVGGFDEGYGGYAGEDTDLGRALAEAGVPVAIVADAVVLHQHHDSYEPP
                     VQQLRATVANAQRYRDKWGAWPMAGWLQGFADLGLVEWSPDASTCTVRRDPSPDELAA
                     CRRRVALPFEEVPA"
     gene            10457..11206
                     /locus_tag="VLR27_11280"
     CDS             10457..11206
                     /locus_tag="VLR27_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HSP04078.1"
                     /translation="MSGSVRDVVVVGAPRSGTSMLAGLFADAGHCAGRRLIPPTPSNP
                     AGFHEDLDVNAANDDLLAALDDTPWPGTVSPIRRLRWIGAYDGWVRGDRSGDDLAVLV
                     PDRPFVLKDPRFCYTLPAWQDVLADVLVVVIVRHPAEVVASVRAMAEREPATFEGFDV
                     TDEHLLAMWTAMHHAVLGWCDTDAPGPVTFVDCDDVRSGAALPVLSRLSGVDLVARTV
                     QPGLHRERSDRDDPSGELGALLERLRERMRG"
     gene            complement(11336..12202)
                     /locus_tag="VLR27_11285"
     CDS             complement(11336..12202)
                     /locus_tag="VLR27_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP04079.1"
                     /translation="MPSVDVNGITIEYERSGAGEPLLLVMGLGAQLTAWPVEMVELLE
                     RAGFDVIRFDNRDIGLSSACDWEPPSPVRAFLGRLLRRPAPTGYVVDDMADDAAGLLD
                     ALGIDRAHVVGASMGGMIAQGLAIRHPQRVASLTSIMSNPGDGSGGATARVLLAFARR
                     DEPTPENAVDQAVATFKRISGPHFREHEYRPLAEAAVARSFRPQGVARQTAAIMASAD
                     RTGGLSRLAVPALVVHGLIDPLVKPSGGIATAQAIPGSRLLMFNDMAHDLPAPRLPEI
                     VDAIARVAAPGA"
     gene            12293..12793
                     /locus_tag="VLR27_11290"
     CDS             12293..12793
                     /locus_tag="VLR27_11290"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HSP04080.1"
                     /translation="MSTHVTHVPVDAAAATAFLRSGRLAVIGASDERDSFGRTVYEAL
                     REQGIEVVAVHPRGGTVAGDVCHPSLLDVPGQLDGAIVMVSAPRSVEVVRQVAARGIP
                     RVWLFRGVGAAGAASPEAVAVAEELGLEVVPGACPLMFLEPVGLVHRFHRGVRRARGQ
                     LTVAAG"
     gene            12857..13840
                     /locus_tag="VLR27_11295"
     CDS             12857..13840
                     /locus_tag="VLR27_11295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HSP04081.1"
                     /translation="MTSAPAYAHDAAPSELEVLTRRLLELLGRITGLESTYLTSIDWD
                     GDWQQVLYARNVGSLDIPEGLQVEWSDTLCRRALEGGPSCTTDVAGTYPDSAAARELG
                     LSTYVTVPVRGPDGEIFGTVCGADSRPIDIDDDAHAVMTTLAEMVALQLANDAARREL
                     VAANAALSELAYLDGLTGLGNRRAFDRALPRACAGTEPIALVAVDIDHFKQINDTLGH
                     AAGDEVLHAISRRLVTHSRAGDTVARLGGDEFVAVLTGADLDTAAAVAERLRADVSGS
                     PIDTVAGPVHVTLSVGVASGTGEAATSLLRRADEALYTAKSAGRDAVHAPA"
     gene            complement(13861..14703)
                     /locus_tag="VLR27_11300"
     CDS             complement(13861..14703)
                     /locus_tag="VLR27_11300"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HSP04082.1"
                     /translation="MEPRTDPPSTPPGGPADRSRTTWALAGLAALVVALVVVLIVVLS
                     GDDDDDDVATEDTTTSVVDSTTTTEVDETTTTAAEEETTTTTASTTPGSVTDAEAATV
                     AWPDPLEGAGGDDPVAVATEFASEVLGFSDPVVGELQEGDSRSGEVEVRAAEDGPVTT
                     VGVRQLSDDRYYVLFAASSEVELLVPTAGSAIDTPLEVEGWGRGFEGQIAIRVHDRSD
                     GAQLGEGFVTGGGVGELEAFSGEIDWDNPGGGWGLVVAAVPGGEDGATWAAAVVPVGF
                     IGGD"
     gene            14860..15726
                     /locus_tag="VLR27_11305"
     CDS             14860..15726
                     /locus_tag="VLR27_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSP04083.1"
                     /translation="MARFIVGVDGSDSSADALRWASREAEVRGATVTALLAWNFLDQY
                     HLDEDTTFDPHYGEDEARRALVAAVQRALGERAADVQLEVVCDLPATALLDASGDADL
                     LVVGARGLGSFRGLVLGSVSQRCLEHATCPVMVVHGTPGARAAHDRVVVGVDGSEHSA
                     TALGWAVEEARLRGAALEVVHAWEVPYAAEFTLVGDAYDAAQRGAEEVVDHMLVAAGL
                     EPDDVVRTVVQASPVLALTDAAAGADLVVVGSRGRGGFAGLLLGSVSHQVSRHADVPV
                     VVVPDPDRSADD"
     gene            complement(15733..17145)
                     /locus_tag="VLR27_11310"
     CDS             complement(15733..17145)
                     /locus_tag="VLR27_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSP04084.1"
                     /translation="MRETAMNTSPSPILSFLGAAGTVTGSRFLVEFAGSSVLVDCGMF
                     QGRRELRRRNWAPFPVDPASIDAVVLTHAHIDHCGYLPALVRDGFRGPVLCSPATGEL
                     VPIMLRDAARLQEEEAAYAERKGYSKHHPPRPLYTGEDAEGAIRLLQAVDFDVEHPVA
                     DGIRARLRPAGHILGSATVELAVGDRRVLFSGDLGRPNHPILRPPAPLPDVDVLVTES
                     TYGGRRHDDMDEGLEQLADLIRRTAARGGSVVVPAFAVDRTEVVLLALGRMVREERIP
                     RLPIYADSPMALAVLDVYRRSMAAGSDEIRPGPWADDPFAAAGELEEVLDTDASKALH
                     DVPYPSIIISASGMASGGRVLHHLAHRLPDPRNAVVLSGFQAPGTRGERLASGADAVK
                     MFGRYVPVRAEVLALRCFSVHADEPETLRWLASTPRPPDVVYVVHGEPEASAAMHDAI
                     SRELGWTAVVASHGERVRID"
     gene            17253..17447
                     /locus_tag="VLR27_11315"
     CDS             17253..17447
                     /locus_tag="VLR27_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04085.1"
                     /translation="MAIELGASLVMLLWAPAARALEHASPPLEGWLVAVATAVVLVAV
                     DAVDKAQRRRTQLDGADGPP"
     gene            17518..>19476
                     /locus_tag="VLR27_11320"
     CDS             17518..>19476
                     /locus_tag="VLR27_11320"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HSP04086.1"
                     /translation="MGTEPSSADASSPFAGTTPVPSSATVGVVTLVEGQTFCVSGRAG
                     DITADFPHGLFLLDTRIVSSWSLQLNRHRVEPLGVDVHEPFGATFLGRSAPAEGHADA
                     DVVVLRHRHVGQGMRERIIVRNHGLRAAPMVLHLSVDADFADLFAVKEGRARALGQIR
                     SEADDGSLRFEQRSDAGSKDVRITASPPGIVEPGSIAWEQTLEPGASFEVCIQVTATI
                     RGIAVEPRFLCGQQDGESLPRQRMATWHATLPKVETASAPLAQTLARSAEDLGALRIF
                     DAEHPELPILAAGAPWFMTVFGRDSLLTAWMTLIADPTLAHGVLETLARFQGRDVDPR
                     TEEEPGKILHEMRFGSTTNLALGGGDVYYGSIDATPLFVMLLGELRRWDARDELVDRL
                     LPHADRALAWIDEHGDRDGDGYVEYQRSTDTGLANQGWKDSWDAVRHRDGRLATTPIA
                     LCEVQGYVYAAYVARVHLAREAGDTATAERYAAKASTLRRRFNEDFWSDEHGWYVLAL
                     DGDKRPVDVLSSNIGHCLWSGIIDPARAGLVAERLLSADMFSGFGVRTLSASAAAYNP
                     VSYHNGSVWPHDNALCAAGLARYGFVDAAHRIIEAQLDVARAHDGRLPELFTGFSRDE
                     FAVPASYPASCAPQAWAAASPMLWLRTLL"
BASE COUNT         2456 a         7291 c         6974 g         2755 t
ORIGIN      
        1 gcctccttca cgtgggtgcc gggcctggac ctgtccgtgg acctgttcgt cgacgggctc
       61 ggcctggtca tgatcctgct ggtgtcgggg atcggcctcg ccatcgccgc gtactccggc
      121 tcctacttcg cgcccaacgc caccgccgcc cgcacggccg ggctcctcgt gctcttcgcc
      181 ggagcgatgc tcggcctcgt cacggccgag cacgtggtga tcctctacct gttctgggag
      241 ctcacgtcga tcctgtcgtg gctgctcatc agccagcgcc acaccgaccc ccaggcgcgc
      301 gccgccgcga cccacgccct cgtgctgacc ggttcgggcg ggctcgccct gctcggcggc
      361 ctggtgatcc tggcgaggga ggcgggcacg tggtccgtcg tcgagctgct cgccgacccg
      421 cccgggggca cggccgccgg cgtcggcctc gggctcgtgg tcctgggcat cgtcaccaag
      481 tcggcccagt acccgttcca cgcctggctg ccgggggcga tggtcgcccc cacgccggtc
      541 agcgcctacc tgcactcggc caccatggtg aaagccggca tctacctcgc cctccggttc
      601 gccccgtcct tcgccgacct cgacgcctgg cagccggtcg tcctcacgat cgggctgctc
      661 acgatggtcg ccgggggcct gcgagccatc cgccaggacg acctcaagct gctgttggcg
      721 ttcggcaccg tgagccagct cggcttcctg ttcgtgctca ccggcctcgg gttcgaggac
      781 gccaccatcg ccggcgtcac gctcctggtc gcccacgccc tgttcaaggc cacgctcttc
      841 ctcgtcgtcg gcatcgtcga ccaccaggcc cacacgcgcg accgccacga gctgctcgac
      901 ctgcgcctcg gcaacgggtg ggggccggtc cgggtgtgcg ccgtgctgac cgccgcctcc
      961 atggccggcg tccccctgct ggccggcttc atcgccaagg aggaggccta cgccgcgttc
     1021 gtgtacgagg gcggcaccct cggcgtgatc gggctcgtcg gcatcgtcac cggctcgatc
     1081 ctgacgatgg cctacagcct ccggttcgcg tccgtcttct ggcgccagag cgaccgggac
     1141 gacgccgacg tcaacccgcc gtcctggacc ttcggcgccc ccgtctacgt gctcaccgcg
     1201 ctcaccctcg tcatcggcct ggtgccggca ccgatcatcg gcgacctggt ggacgtggcc
     1261 gccggcgacc tcctcggcgt cgaggtggcc tcgaagctcg cgctctggca cgggatcaac
     1321 accgccctga tcctgtcgct cgtcaccatc gccgctggcg tcctcctgtg gcgactcacc
     1381 gaccccttcg tccgcctcac cggtcgggcc gccccgccga tcgacggcta ccgggccttc
     1441 cagatcggcc tcgagggcct gttcaccgtc gctcgcgtga ccaccacgcg gatccagacc
     1501 ggctcgctcc cccgctacgt ggccatcatc ttgatggtgg tcgtcggcgg accggctctg
     1561 gtcgccgtgg ccaccacgtc gatgcccgac ctcgccgacc cgacggacgt ccccgtgcag
     1621 ctcgccctcg ccgggttcat cgccggcgcc gcgctggcca ccatcttcgt gccccgtcgc
     1681 ctcggggcgg tcgtggtgct cggcggcgtg ggctacggga tggccggcct gttcgtggcc
     1741 cagggcgcac ccgacctggc gctcacccag ttcgcgatcg agaccctcac gatcgtggcc
     1801 ttcgtcctcg tgctccgcca gctgcccgcc cgcttcggca cggacggcga ccgggccggg
     1861 aaggcccggc gcctcggcgc ggccgcggtc gtcgggctgg gcgccttcgt gctcgtgctg
     1921 gcggtgagcg gcaccgaccc gaccacgccg gcatcggacc tggcgatcga gcaggcggtg
     1981 ccggaggccg aggggcgcaa catcgtcaac gtcatcctcg tggacttccg aggcctcgac
     2041 accctcggcg agatcgccgt gctgctcgtc gccgccgtcg gcgtcaccac cctggcgcgg
     2101 gtcggccggc gcccgccgcg caccggcgtc ccgccgctcc ccgagatcga cccgctcgac
     2161 ccgaccacga tggacaagga cgcccggacg tgaagcggtc cgtcatcgtc gacgtcaccg
     2221 cccggctgat cctgccgtcg gcgctgctgt tcgccctgca cctcctgctc tccggccacg
     2281 accggcccgg gggcggcttc gtcggcggcc tggtggccgg cgccaccatc gcgctgatct
     2341 tcgtcgccgg cggcatggac gacgtgcgct cggtcgtgcc cgtccggccc tggacgatcc
     2401 tcggcggcgg gctggcgatc gccgcctgcg tggcgacggt gccgatcctg ctcggcaacc
     2461 cggtcatgga caactggaag ttctacgtcg acgtgccgct cctgggtgag gccaagatca
     2521 gcagcgccct ggtgttcgac atcggcgtcg cggccgtggt gatcgggacg atcttcatgg
     2581 tcttcgaggc cttcggcgac gaggagcccc ccgagttccg cggcgaggtc cgcgagggtc
     2641 ccggcagcgg cccccgccgg gaggtcgggt cgtgaccacc gtcttcgccg ccctcgcggg
     2701 cgccctgttc gccaccggca cctacctggt gctccagcgc gagctcaccc gcatgctcat
     2761 cggcctcgcg ctgctcagcc acggcgccaa cgtggtgctc ctcgtcgcgg ccggggatcc
     2821 cgggcggccg gccttcctgg acgggcgaga cgagccctcg gcgttcctcg accccctccc
     2881 ccaggccttc gcgctgaccg ccatcgtcat caccttcggc gtcacgtgct tcctgctcgc
     2941 cctcgcctac cggagctggg tcctcaccca ggacgacacc gtgcacgacg accccgagga
     3001 cgtgcgcgtg cgcgaggagc ccgacgccga catcgagcgc tccggggtcg atcagcccac
     3061 catcgaggcg caggaccgat gaccccctgg atcgccctcc ccgcggtgct gccgctgctg
     3121 gccgccgccg cgtcggtcct ggtcggccgg tcgcgcaccg cccagcgcat catcgggctg
     3181 gggacgctca ccgccctgct cggcctcgcc gtgcgcctgc tcatcgagac cgaccgcaac
     3241 gacgagatga ccacccaggc cggtggctgg gtggcgccgc tcggcatcac cttcgtcgcc
     3301 gaccgcctgt cggcgatcat gctgctggtc gccatcggca tgttcctggc cgtgctcctc
     3361 tacgccatcg gccagggcga cgaggagagc cgccacgtcg ggttccaccc cgtgtacctc
     3421 acgctcgtcg ccggcgtgtg catggccttc ctcaccggcg acctgttcaa cctgttcgtc
     3481 gcgttcgagg tcctgctggt ctccagctac gtgctgctca ccctgggcgg acgcctcgcc
     3541 caggtccggg ccggcaccac ctacgtggtc atcagcctgc tggcctccat caccttcctg
     3601 atgtcgctcg ggctgctcta ccgggccacc ggcacggtga acctggccga cctgcccgca
     3661 gcgatggagg tgctcgaccc cgagctgcgc cgggtgttcc tcctgctgtt cctcggcgtg
     3721 ttcggcatca aggccgccat cttcccgctg ttcttctggc tgccggacag ctaccccaac
     3781 gcaccgacgg cgatcacggc ggtgttcgcc gggctgctca ccaaggtcgg cgtgtacgcc
     3841 atgatccgca tccagaccac gatcggcccc gacgacaccg tcgtgggcac ggtcctgctg
     3901 gtggtggcag ccctcacgat ggtgatcggc gtgttcggcg ccatctccca gcaggagatc
     3961 aagcggatcc tgtcgttcca cgccgtcagc cagatcggct acatgatctt cggtctcggg
     4021 ctgctcaccg tcgccggcct cgccgcagcc atcctctaca tcacgcagta catcgtcgtg
     4081 aagaccaccc tgttcctcac cggcggcctg gtcgagcgcg cctcgggcac cggtcacctc
     4141 gaccgcgtcg acggcatgct ccgcacccgc ccgctgctcg gcatgctctt cctgctgccg
     4201 gccctgagcc tggccggggt ccccccgctg tcgggcttcg tgggcaagtt cgccctcgtc
     4261 gacgccggcg tgtcgtcccg gagctggatc ctggtgggag tgagcctggc ggtgagcatc
     4321 atgacgttgc tgtcgatgaa caagatctgg gccggcgcgt tcatgggcga accgacggcg
     4381 aacgccgagg cgacccaccc gctgcccacc gcgatggtct tcgccaccgg cgtgctcgtg
     4441 gtggtgagca tcggcatcgc cctcggcgcc gggcccatct acgccttcgc cgagcgggcc
     4501 gccgagaccc tgctgccggt cgccgggagt gccgcgccat gagctacgtc ggccccgtca
     4561 tctggctcac cggactgtgg atgatcctct ggcgcagcac cacgcctggc accatcgtca
     4621 ccggcatcgt gctggcggtc gccctcacct gggtggtgcg gcggtccgac gtccaccggc
     4681 gccaccaccg cgtgcacctg ctcccgctgc tccgcttctt cgggcacatg gcggtcgccc
     4741 tggtgaagtc caacatcgcc ctgggcttcg aggtgctcac ccccaccgac tacacgaccc
     4801 ccggcctgct cgaggttcgc ctgcccacct gctccgagct ggtcctcacc gtcatcgcca
     4861 acagcatcac gctgacgccg ggcacgatga ccctcgagct gcgacccgac acctccacgc
     4921 tcctcgtgca cgtgctgcac ctgcgaaacg tggacgccgc acgcgacgag atcctcgagc
     4981 tgcatcggct ggtcagcgcc gccctcgtcc cggtggcgac gcccgacgag cgaggagccg
     5041 agatcacgtg atcgtcgcca ccttcgtcct gctgtcctgc gccgccgcgc tcttcgcgta
     5101 ccgcgccgtg cgggggccct ccatcgtcga ccgcgtcgtc gcggtcgacg ggctggtcat
     5161 catcgccatc ggcgcgatcg ccgccgatgc cgtccgctac gacgacgccc gcttcgccga
     5221 cgccgccatc ctgctcgcca tcatcgcctt cgtcgggacg ggcatcgtcg cccgcttcat
     5281 cgaacgtcgg ggaggttgac cgtggacctc gtcgccgacc tgatcatgct ggccggggcc
     5341 ttgtggttcc tcctcgccgg gctcggggtc gcccggctgg gcgactccct cgcgcgggtg
     5401 cacgccgcca ccaaggcgac caccctcggg atgatgctgg tcgtgctcgg cgccgcgctg
     5461 cagctgccgc tcgacgacgg cctgcgcatc gcgctggcca tcttcctcat cgccctgacc
     5521 aacccgctcg gcggccacct gctgggccgg gcggtcgagg cgaaccccgg caccgccgag
     5581 atgcgcatcg acgtccgggc gacgctggac gacgacggcg gcgacgacga cggaagcctc
     5641 gagcccgacc agggctgagc gagctcaggc cgctgggcag ccgcgatcgc aaccgccggt
     5701 ggcgtggacc cacgcccggc gcacctcgtc gttccagtcg tcgcgctgca cccggaggcg
     5761 ggtggccaca tcgggtcgtt cgggaagctc gacgccgatg agtcccgcag cgacggacgc
     5821 gatgcagctc ggatcccggg cgacgtcttc gtagacgacc tcggcccacg ggatcgccgt
     5881 ggtcaggacc agtgcccgcc accggtcggc accctcgtgc tgggcgtcgt ggagccggga
     5941 cacccggtcc aggtcaggcg gtcccggcat cggacggtgc tcggtcgcgg cggtggacca
     6001 ctcccccgtc tgctcggctc ggtgctggga gatggcggcg gcgagtcggt cgcggcgggt
     6061 caccagcagc gccaccgggt cgacgaagca gtcgaagacg tcggcctcgt gccggtcccc
     6121 ggcgcgggcg agccgggaga cgtcatccca catcagcttc accgcgcaga tgccgttcgg
     6181 ggtcgtggcg ttcgtgaacg cagcgcggac gtagcggtgc gggtcctcgt cgagcgtggg
     6241 gacggcccag gcatcggcgc aggccgggat ggcgacctcg gcgaagggct ccttggggaa
     6301 cccgagcagg ccggtgccgg tgagcacgtc gcaggcgagc gtgctccccg tgcggggcgt
     6361 ggcgcacagg acgatggtgc gcttcggcgg ccaggtcgtg ccccgctcgc gtcgttcggc
     6421 gctcacgacg tgaccgcctc gtagatctgg gcgtgcgccg cggccaggtc ctggcgctgc
     6481 tgggtccgcc actcccggga cagcgccggc tcgtcccggt gcgcccagca cgcgtccagg
     6541 acgcgggcga gcgccgtgcc cacgtcgggt gcgtcgaggt cgagcgcgtg gatgctcggg
     6601 tgctggtcgg ggatgcacgt gctcgtcggt gccacgacct gggtgccgac atcgaggcag
     6661 gcctcggccc accccgagtg ggtgccccac cggtacggga gcagggcgac gtcgatgctg
     6721 cggacgtagg cctcgaactc gtcgtcgggt ccgtaggggc gcagctggag ctccacgtcg
     6781 ggcgcggcgg ccagctgctc gaggcgccga ccggcgtcgg gctggcgctc gacggcggcc
     6841 gggtaggcgt cgacgcgcag gcggaaccgc tcgtccccac cgaccacgtc ggccacgacc
     6901 tcccacagct cgggtccgtg cacggcggcg cgcaggtcct tcaggtgcat gccgacgacc
     6961 agcggagccg tgcgcacccg gggctccccg acccgttcca gctccacgat gtgggggtgc
     7021 gggagcacca ccggacgacg gccccagtcg cgctcgacgc gatcggcggc acatccggtg
     7081 agggtgatga gcgcgtcggc ggccggcacc agcaggtcca gctgccggcg gtggtggcgg
     7141 tgatcggcgc ggtgcgggtt ctcgaggtcg tgcacggtgt gcacgagcgg gcgtccgacc
     7201 tcgacgagcg cggcgagcca cgcccgcatc gacgggaggt cgacgtcacc gaagccgaag
     7261 tgggtgtgca tgcagtcgaa ctcgtcgcgg ttcgcccgca cccagtcggc gtcgaccaga
     7321 tcactggccc ggtacgtggc atcgggcgtg gcgatgcgcg ccacgccgtc gcccggccaa
     7381 ccgatgtggc gggtgtagac gtggcgacgg ggcacggacg ccaccctcac gcgacccggc
     7441 cccggtgccc ggccgcgacg tcgccgaggt gcgcggcgat gcgggcggcg ccgtcggtcc
     7501 cgagcgaggc cagcacgccc ggatccaacc cgtccgcgtg ctggagcacg gctcgccacg
     7561 cccccggttc tggccagcga tcgagcacga cggcggcccc ggcggcctcg agggcttcgg
     7621 ccttgtagcg gtgctcgtcg aagggacggg gttcgcacac ggcgacgaag cgggcgccgg
     7681 caccggccac gtcccccacc aagccgtcgc ccggcgggcc gatcacgacg tccccagtgg
     7741 cgatgcacgc cgccacgtcg tcgacccagc cgtggtgccg gacgtgctcg ggcaccccgt
     7801 cggggcacgg cggtccgacc atctcccagg tccggcccgg tgtcgccgcc gcggcggcgt
     7861 cgagggcagc agccggcggg ggcgggtggc cctggcccca cacgacgagc acccgaccct
     7921 ggccaccggg gtcgcggtcc gggccggtga accgggtggc cggggtgacg aagccgctgt
     7981 ggaccgtctt gacccgcacc cacgccggcg tggaggggtg ctccagctcc gccgggaacg
     8041 gggccacgat cgacctggcg aggcggtggg cgaggtcgtg gggggcgtcc gcgcggtcgc
     8101 cgtgcaggcg caccaccacg acgggcacgc ccgcgaggcg gacggtgagg gcgacctcga
     8161 ccgacacgtc gatcagggcg acggagggac ggcggtcgag gagccacccc gtgatggcgt
     8221 gggtccgtgc tgcgcccacc tcgtgttcgg gggcccagtg gagcgcgccg ccggcggtga
     8281 cgtcgccggc cggtccgggc ggcacatcgg tcgggagcgg gcagacgtcg gccccgcgtg
     8341 gcagcgcgtg gcgggcgtcc gggtggctcg tggccacggt gcaccgctcg ggcccgagcg
     8401 cctcgcagac cgcggcgatc cgggagaggt ggccgcgacc ctggtggtgc gcgtagaagg
     8461 cgaagggtcg cggatccgtc atggccgggc gcacggatcc gacgtgcacc ggcggaaggc
     8521 ggcggcgtag cgatcgacca tcgtgtcgag cgagaaccgc tcgatggcgt ggcgacggca
     8581 ggcatcgcgg tcgaggtcgc gcgcctccag gatcgctgcc gcgagaccgg cgacgtcgcc
     8641 caccggggcg aggcgaccca ccgcgtcgtc caccagctcc ggcaccgcac cggcggcgaa
     8701 gccggccacg ggggtcccgc acgcgagggc ctcggcgacg acgaagccga acggctcggc
     8761 ccagacgggc gtgaccacgc agacctcggc accggcgacg agctcggcac aaccctcggg
     8821 cgacaggtgc cccaggtagc ggacctcgtc ccccaggcgg ggctggatct cgcgctcgaa
     8881 gtagcaccgg tcgtgcatcg gtccggccag gtcgatggtc agccccgccg cccgcgccgc
     8941 gtcgatggcc aggtgcggtg ccttctcggg caccagccga ccgaaccagg cggcaccctc
     9001 ggggcggacg ccgaggcggg gccgccagag gtcgcagtcg acgccgttgc cgatgacctc
     9061 gtcgacggtg cccggagccc actgcgctgc gttggccagg gacaccgaga cgacatggcc
     9121 cgtcttgggt ccggcggccg ccagcgccga ctcgagccac ggggtcggcg gggtgtggag
     9181 ggtcgtcacc atcgggaccg gcacgagcgc cgccatcgcg agcggcagca ggtgggtgct
     9241 gttgttgtgc accacgtcga gctcgacctc gccgagggcg agcgaccgga ggatccgcag
     9301 gtacgcgtgg tgctcgctca cgacctcggc cgggacggag ctgacgtcgg cgcgggcgac
     9361 ctcgctgggc gcccagtcgg ccgtgaccag gcggaccgtg cagccctcgg gtgcgtccgt
     9421 cccgaacagg gtcacctcgt gaccgcggtc gcggaggccc tcggccagca tccacgtgtg
     9481 cgcctccagc ccgccggcga agggcgggcg ctgcgggaag cggacgctgc cgatcaggcc
     9541 gatgcgcagg gggagctcgc tcacgccggc acctcctcga aggggagcgc gacccggcgc
     9601 cggcacgcgg cgagctcgtc cggggagggg tcgcgccgga ccgtgcacgt cgaggcgtcg
     9661 ggggaccact cgaccaggcc gaggtcggcg aagccctgca accagcccgc catcggccac
     9721 gcaccccact tgtcccggta gcgctgggcg ttggcgacgg tggcccgcag ctgctggacc
     9781 ggcggctcgt aggagtcgtg gtgctggtgc agcacgaccg cgtcggcgac gatggcgacc
     9841 gggacgcccg cctcggccag ggcacgtccg aggtcggtgt cctcgccggc gtagccgccg
     9901 tagccctcgt cgaacccgcc gacggactcg aacgtagagt ggcgcatcgc catgcagagc
     9961 ccccacacca gctcgtgacg gtcgctcagc accacgccgt ggtccggcac ggtctcgcgg
    10021 tgggggtggg gtcgggagcg ccgcagcaac gtggggaagt ccagcccgga gacgtcgccg
    10081 ggtggcaggt agcggacctc gcccgtgcac agcgcgtcgt ggcgggccag cgccgcgtcg
    10141 atggcgccgg cgaaggtggg cgatgcgatc gtgtcggcgt cgcagaaggc caggtggtcg
    10201 ccggatgccg ccgccgcacc ggcgttgcgc gcctcgctgt acgggatgcg gtcatcgggt
    10261 gcgccgacct cgcagaccgt cgcccgcagc ccatcgagct cggcgacgac ctcgcgcggg
    10321 tcctcgtcgc ctccggcgcg caccaccacc acctcgaccg gcgcgtcctg ggcggcgacg
    10381 cccgcgagga acgagcggag cagggcgttc cggttccgga cgagggtcac catgctcagc
    10441 acggagccac cccctcgtga gcggctccgt ccgggatgtg gtggtcgtgg gcgccccgcg
    10501 cagcggcacg tcgatgctgg ccggcctgtt cgccgacgcc gggcactgcg ccggccgccg
    10561 cctcatcccg ccgacaccgt ccaacccggc cggcttccac gaggacctcg acgtcaacgc
    10621 cgccaacgac gatctgctcg ccgcgctgga cgacacgccg tggccgggga cggtgtcccc
    10681 gatccgtcgg ttgcggtgga tcggggcgta cgacggttgg gtgcgtggcg accgttccgg
    10741 cgacgacctc gccgtcctcg tcccggaccg cccgttcgtc ctgaaggacc cgcgcttctg
    10801 ctacacgctg ccggcctggc aggacgtgct cgccgatgtg ctggtcgtgg tgatcgtgcg
    10861 gcacccggcc gaggtggtgg ccagcgtccg ggccatggcc gagcgggagc cggcgacctt
    10921 cgagggcttc gacgtgaccg acgagcacct gctcgccatg tggacggcca tgcaccacgc
    10981 cgtgctgggc tggtgcgaca ccgatgcgcc cgggccggtg accttcgtcg actgcgacga
    11041 cgtgcgcagc ggggcggcgc tcccggtgct ctcgcggctc agcggcgtcg acctggtggc
    11101 ccgcacggtg cagccgggcc tccaccggga gcgctcggac cgtgacgacc cgagcgggga
    11161 gctgggcgcg ctgctcgaac ggcttcggga gcggatgcgg gggtgagatc gccggtcgac
    11221 gatgaccggc agggggcctg ggggacatgc atcgtcctgc gctcgtgctc gggtttcgag
    11281 agcgcgcctc ctaccccgat ggcccacgac cagaactccc ggggccgacc agtcgtcagg
    11341 ccccgggcgc ggccacccgg gcgatggcgt cgacgatctc gggcaggcgc ggtgccggca
    11401 ggtcgtgggc catgtcgttg aacatgagca ggcgggagcc ggggatggcc tgcgcggtgg
    11461 cgatcccgcc gctgggcttc accagcgggt cgatcagccc gtgcaccacg agcgccggga
    11521 ccgccaggcg ggacaggccc ccggtgcggt ccgcgctcgc catgatcgcc gcggtctggc
    11581 gggcgacgcc ctggggtcgg aagctgcggg ccacggccgc ctcggccagc gggcggtact
    11641 cgtgctcccg gaagtggggc ccgctgatgc gcttgaacgt ggccacggcc tggtccacgg
    11701 cgttctccgg ggtcggctcg tcgcggcggg cgaaggccag cagcacccgg gcggtggcac
    11761 ccccgctgcc gtcaccgggg ttggacatga tcgaggtcag gctggcgacc cgctgggggt
    11821 ggcggatggc gaggccctgg gcgatcatgc cgcccatcga ggcgcccacg acgtgggccc
    11881 ggtcgatgcc gagggcgtcc aggaggccgg cggcgtcgtc ggccatgtcg tcgacgacgt
    11941 agccggtcgg cgcggggcga cgcagcagcc ggccgaggaa ggcccgcacg ggtgacggcg
    12001 gctcccagtc gcaggccgag ctgagcccga tgtcgcggtt gtcgaagcgg atcacgtcga
    12061 agccggcccg ctcgagcagc tccaccatct ccacgggcca ggcggtgagc tgggcaccga
    12121 ggcccatcac cagcagcagc ggctcgccgg ccccgctgcg ctcgtactcg atggtgatcc
    12181 cgttgacgtc gaccgaaggc atggggccag tgtggcggga ccttcgcctc tgtcccggat
    12241 cgggcaggtc gtcctaccgt cggaggcagt cgaacgaccg aaggagcacg tcgtgtccac
    12301 ccacgtcacc cacgttcccg tcgacgccgc ggcggcgacg gcgttcctgc gatccgggcg
    12361 actcgcggtc atcggtgcct ccgacgagcg cgacagcttc ggtcgcaccg tctacgaggc
    12421 gttgcgggag cagggcatcg aggtcgtcgc cgtccacccc cggggcggga cggtcgccgg
    12481 cgacgtctgc catcccagcc tcctggacgt cccgggccag ctcgacggcg ccatcgtcat
    12541 ggtctcggcc ccgaggtcgg tggaggtcgt ccgccaggtc gcggcccgag gcatcccgcg
    12601 cgtctggctg ttccggggcg tcggcgcggc cggcgccgcc agcccggagg ccgtggcggt
    12661 cgccgaggag ctcggcctcg aggtcgtccc cggcgcctgc ccgctcatgt tcctggagcc
    12721 ggtcggcctc gtccaccggt tccaccgcgg ggtgcggcgc gcccgcggcc aactgaccgt
    12781 cgccgccggg tgacgagcgc cggtccgtga ccggcgccac ctcaagtcgc cgtcagcggg
    12841 agccgatgct cctcgcgtga cctccgcccc cgcgtacgcc cacgacgccg ctccgagcga
    12901 gctcgaggtg ctcacgcgcc ggttgctgga gctcctcggt cgcatcaccg ggctggagag
    12961 cacgtacctc acgtccatcg actgggacgg ggactggcaa caggtcctct acgcccgcaa
    13021 cgtcgggtcg ctcgacatcc ccgagggact gcaggtcgag tggtccgaca ccctgtgccg
    13081 ccgggcgctc gaggggggcc cgtcctgcac caccgacgtc gccggcacct accccgacag
    13141 cgccgcagcc cgggagctcg gcctgagcac ctacgtcacc gtgccggtcc gcggccccga
    13201 cggcgagatc ttcgggacgg tctgcggcgc cgactcccgt ccgatcgaca tcgacgacga
    13261 cgcgcacgcc gtgatgacga cgctggccga gatggtcgcg ctccagctgg cgaacgacgc
    13321 ggcacgccgc gagctcgtcg cagccaacgc cgccctgtcg gagctggcct acctcgacgg
    13381 cctcaccggc ctcggcaacc gccgggcctt cgaccgcgcg ctgccacgtg cctgcgcagg
    13441 caccgagccg atcgccctcg tcgcggtcga catcgaccac ttcaagcaga tcaacgacac
    13501 gctcggccac gctgcaggtg acgaggtgct ccacgcgatc agccggcggc tggtcaccca
    13561 cagccgggcc ggtgacaccg tggcccgcct cggcggcgac gagttcgtcg ccgtgctcac
    13621 cggtgccgac ctcgacaccg cggccgccgt cgccgagcgg ctgcgcgccg atgtgtcggg
    13681 gtcaccgatc gacacggtgg ccggaccggt ccacgtcacg ctcagcgtcg gtgtggcgag
    13741 cggcacgggg gaggcggcga cctcgctcct gcgacgggcc gacgaggcgc tctacaccgc
    13801 caagtccgcc ggccgcgacg cggtgcacgc ccccgcctga ccgtcggacc ggctggcggg
    13861 tcagtccccg ccgatgaagc cgaccgggac cacggcggcc gcccaggtgg cgccgtcctc
    13921 cccgcccggg accgcggcga ccacgaggcc ccacccgcct ccggggttgt cccagtcgat
    13981 ctcgccggag aaggcctcga gctcgccgac gccgccgccg gtgacgaagc cctcaccgag
    14041 ctgcgcgcca tctgaccggt cgtggacccg gatggcgatc tggccctcga agccgcggcc
    14101 ccagccctcg acctcgagcg gcgtgtcgat ggcgctcccc gccgtgggga ccagcagctc
    14161 gacctcggag gatgcggcga acaggacgta gtaccggtca tcgctcagct ggcggacacc
    14221 gacggtcgtc accggtccgt cctcggcggc gcgtacctcc acctcgccgg agcgggaatc
    14281 cccctcctgg agctccccca cgaccgggtc ggagaacccg agcacctcgg acgcgaactc
    14341 ggtggcgacg gccaccgggt cgtcgccgcc ggctccctcg agcggatccg gccacgccac
    14401 cgtcgccgct tcggcatcgg tgaccgagcc gggcgtggtc gacgccgtcg tcgtcgtcgt
    14461 ctcctcctcg gcggcggtcg tcgtcgtctc gtcgacctcg gtcgtcgtcg tcgagtccac
    14521 gacgctggtc gtggtgtcct cggtcgcgac atcgtcgtcg tcgtcgtcgc cggagagcac
    14581 gacgatcagg accacgacga gcgcgacgac cagggcggcc aacccagcga gcgcccaggt
    14641 cgtgcgggag cggtcggccg gtcctcccgg cggtgtggat ggggggtccg tgcgcggttc
    14701 catggggtga tcacacgcct gtgggcgacg ttgcgccaga gtctcgaggc actgctccgt
    14761 ggggaggatc cgatcccgcc cggccggtga cgttcgcccc tggccgccgc ctgacccccg
    14821 acgcacactc gggggaggac cccccaggag gagaccacca tggcacggtt catcgtcggc
    14881 gtcgacggct cggacagcag cgcggatgcg ttgcgctggg ccagccgcga ggcggaggtg
    14941 cgaggcgcca ccgtcaccgc gctgctcgcc tggaacttcc tcgaccagta ccacctcgac
    15001 gaggacacga ccttcgaccc gcactacggc gaggacgagg cgcggcgtgc cctcgtggct
    15061 gccgtccagc gtgcgctcgg cgagcgggct gccgacgtgc agctcgaggt cgtgtgcgac
    15121 ctgcccgcca ccgcgctgct cgatgcgtcc ggggacgccg acctgctcgt ggtcggtgcc
    15181 cgggggctcg gcagcttccg cggcctcgtg ctcggatcgg tcagccagcg gtgcctggag
    15241 cacgcgacct gccccgtgat ggtcgtccac ggcacgcccg gcgcgcgggc ggcccacgac
    15301 cgcgtggtgg tcggggtcga cggctccgag cactcggcga ccgccctcgg gtgggcggtg
    15361 gaggaagccc gcctccgggg tgcggccctc gaggtggtcc acgcgtggga ggtgccctac
    15421 gccgccgagt tcaccctcgt gggcgatgca tacgacgcag cccagcgcgg cgccgaggag
    15481 gtggtggacc acatgctggt ggccgccggg ctcgagccgg acgacgtggt gcgcacggtc
    15541 gtgcaggcct cccccgtcct ggcgctcacc gacgccgctg ccggcgcgga cctcgtggtc
    15601 gtcggctccc ggggacgcgg cggcttcgcc ggtctgctcc tcggctccgt gagccaccag
    15661 gtgagccgcc acgccgacgt gccggtcgtg gtggtgccgg atccggatcg cagcgccgac
    15721 gactgatcgg gatcagtcga tccggacccg ttcgccgtgc gaggccacga cggcggtcca
    15781 gccgagctcc cgggagatgg cgtcgtgcat cgccgcgctg gcctccggtt ccccgtggac
    15841 cacgtagacg acgtcgggtg ggcgaggcgt ggaagccagc caccgcaggg tctcgggctc
    15901 gtcggcgtgg accgagaagc accgcagcgc caggacctcg gcgcgcaccg ggacgtagcg
    15961 cccgaacatc ttcaccgcgt cggcccccga tgccagccgc tctccgcggg tgccgggagc
    16021 ctggaagccg gacaggacga ccgcgttccg cgggtccggg agccggtgcg cgaggtggtg
    16081 caggacgcgc ccgccggatg ccatgccgga tgccgagatg atgatcgagg ggtacggcac
    16141 gtcgtggagc gccttggagg cgtcggtgtc caacacctcc tccagctcgc cggctgccgc
    16201 gaaggggtcg tccgcccagg gcccgggacg gatctcgtcg ctgcccgccg ccatcgaccg
    16261 ccggtagacg tcgaggacgg cgagcgccat cgggctgtcc gcatagatcg gaagccgggg
    16321 gatgcgctcc tcccggacca tgcgacccag ggcgagcagc accacctcgg tccggtcgac
    16381 ggcgaaggcg ggcacgacga ccgatccccc gcgcgccgcg gtgcgccgga tgaggtcggc
    16441 gagctgctcg aggccctcgt ccatgtcgtc gtgccggcga cctccgtagg tcgactcggt
    16501 gacgaggacg tcgacgtccg gcagcggggc gggcggccgc aggatcgggt ggttcggccg
    16561 gccgagatcg ccgctgaaca gcacccggcg gtcgcccacc gcgagctcga cggtcgcgga
    16621 gccgaggatg tgccccgccg gccggagccg ggcccggatc ccgtccgcca ccgggtgctc
    16681 gacgtcgaag tccaccgcct ggagcagcct gatcgcgcct tccgcatcct cgccggtgta
    16741 gagcggccgt ggcgggtggt gcttcgagta gcccttccgc tcggcgtagg cggcctcctc
    16801 ctcctggagg cgtgccgcgt cgcggagcat gatcggcacg agctcaccgg tggcgggcga
    16861 gcacaggacc ggaccacgga acccgtcgcg caccagcgca ggcaggtacc cgcagtggtc
    16921 gatgtgggca tgggtcagca cgaccgcgtc gatcgaggcc gggtccaccg ggaacggtgc
    16981 ccagttccga cgccgcagct cgcggcgacc ctggaacatg ccgcagtcga ccagcacgct
    17041 ggaaccggcg aactccacca ggaagcggct cccggtgacg gtgccggccg cgcccaggaa
    17101 cgacaggatc ggtgacgggc tggtgttcat cgcggtctcc ctcatcgggt cggtgcctca
    17161 gcgcaccccc ggatcgtgac gtcgcgccac gggcaccacc agaggcatgg gtcccgacct
    17221 cgcacggtcg caacctcctc ctgccgccag cggtggccat cgagctcggc gcctcgctgg
    17281 tcatgctcct ctgggcgccg gccgcccggg ccctggagca cgcatctccg cccctggagg
    17341 ggtggctggt ggcagttgcg accgcggtcg tcctcgtcgc cgtcgacgcc gttgacaagg
    17401 cgcagcgccg gaggacccag ctcgacggcg cggacggacc accatgacga ccgtcggtcg
    17461 gacgggaagg cgttttggcc cttgcgcaac gccgtcggtc gcgccagcgt catccacatg
    17521 ggaacggagc cgtcatccgc cgacgcatcc tctcccttcg ccgggaccac gccggtgccg
    17581 tcgtcggcca ccgtgggcgt cgtcacgctc gtggaggggc agaccttctg cgtctccggg
    17641 cgggcgggcg acatcaccgc cgacttcccc cacggcttgt tcctcctgga cacccggatc
    17701 gtgtcgagct ggtccctcca gctgaaccgg caccgggtgg aaccgctcgg ggtcgacgtg
    17761 cacgagccct tcggcgcgac cttcctcggt cggagcgcac cggcggaggg ccacgccgat
    17821 gccgacgtcg tcgtcctgcg ccaccgccac gtggggcagg gcatgcgcga gcgcatcatc
    17881 gtgcgcaacc acggcctccg agccgcgccg atggtcctcc acctctccgt cgacgccgac
    17941 ttcgccgatc tgttcgccgt gaaggagggc cgcgcccggg cgctcggcca gatccggtcg
    18001 gaggcggacg acggctcgct gcgcttcgag cagcggtcgg acgcgggttc gaaggacgtg
    18061 cgcatcaccg cctccccacc cgggatcgtc gagccgggtt cgatcgcctg ggagcagacc
    18121 ctcgagcccg gcgcctcgtt cgaggtgtgc atccaggtga cggcgacgat ccgcggcatc
    18181 gccgtcgagc cgagattcct gtgcggccag caggacggcg agtcgctccc gaggcagcgc
    18241 atggccacgt ggcacgccac cctgccgaag gtggagaccg ccagcgcgcc cctggcccag
    18301 acgttggctc gctcggccga ggacctgggc gccttgcgca tcttcgacgc cgagcacccc
    18361 gagctgccga tcctggccgc gggggccccg tggttcatga ccgtgttcgg gcgcgactcc
    18421 ctgttgacgg cctggatgac cttgatcgcc gacccgacgt tggcgcacgg ggtgctggag
    18481 accctggcgc gcttccaggg ccgggacgtc gatccgagga ccgaggagga gcccgggaag
    18541 atcctgcacg agatgcgctt cgggtccacc acgaacctgg ccctcggtgg tggcgacgtc
    18601 tactacggct cgatcgacgc gaccccgctg ttcgtgatgc tgctcgggga gctgcgcagg
    18661 tgggacgccc gggacgagct cgtggatcgt ctgctccccc acgccgaccg cgccctggcc
    18721 tggatcgacg agcacggcga ccgcgacggg gacggctacg tcgagtacca gcgatccacg
    18781 gacaccggcc tggccaacca gggttggaag gactcctggg acgccgtgcg ccaccgagac
    18841 ggcaggctcg cgacgacgcc gatcgcgctg tgcgaggtgc agggctacgt ctacgccgcc
    18901 tacgtcgccc gggtgcacct ggctcgcgag gccggggaca ccgccactgc ggagcgctac
    18961 gccgccaagg cgagcacgct gcgccgccgg ttcaacgagg acttctggtc cgacgagcac
    19021 ggctggtacg tgctcgccct cgacggggac aagcggcccg tcgacgtcct gtcgtccaac
    19081 atcggccact gcctgtggag cggcatcatc gacccggcgc gcgccggcct cgtcgccgaa
    19141 cggctgctct cggcggacat gttctccggc ttcggcgtcc gcacgctgtc agcgtcggca
    19201 gccgcctaca acccggtgag ctaccacaac ggctccgtgt ggccccacga caacgccctc
    19261 tgcgcggccg gcctcgcccg gtacggcttc gtcgacgccg cgcaccgcat catcgaggcc
    19321 cagctggacg tggcgcgcgc ccacgacggt cggctgcccg agctgttcac cggcttctcg
    19381 cgggacgagt tcgccgtccc cgcctcgtac ccggcctcct gcgcgccgca ggcgtgggcg
    19441 gcggcatcgc cgatgctctg gctgcgcacc ctgctc
//
LOCUS       DATISU010000283         4354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_204351, whole genome shotgun sequence.
ACCESSION   DATISU010000283 DATISU010000000
VERSION     DATISU010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204351"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VLR27_11325"
     CDS             complement(<1..837)
                     /locus_tag="VLR27_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSP04087.1"
                     /translation="MLSETVLHHLLDQSADVTVLVDASTGRSIHRWYDPTTSRLHLPG
                     SPMARAITFIHPDDLPAVLDVLAGAAHEGGSRSTVARVHPDHAAEVGSSLMIVAHDVS
                     ASVPFGLLVHAWLVDFDAAALEGAEAGMAMSSLAEAAPVGLQVRSGNGTVSFENSRFS
                     ELVGAGRDEVEARIRAALDSTSTDAGTGAEAGEVSDLAIDGRSLRLRVVPTRDAEHRL
                     LLAVASLEDVTSQRAAEGLFRAVFDGSPVATAVVDLAGRFVQVNDALAMICGRTADDL
                     VGR"
     gene            complement(933..2249)
                     /locus_tag="VLR27_11330"
     CDS             complement(933..2249)
                     /locus_tag="VLR27_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP04088.1"
                     /translation="MVVDRRAELIDAGIRLLGRQQFQALLAAVETRSIADEAGVTTGS
                     FFHHFRNRSHFALAVADAFVSRWAERVDRLEGAATASNDREGYDGIRPAAAEEWAGLV
                     AEEDLSALQHLLWSVRRQELGAETARTAGDVLHDAYRGLTDSVEATYLEGVRRIGREM
                     LPPFTSCDLTVIMTALAEGLQMRHGADPESVREGLYADAVSAVLLGITRPRVERSEAD
                     PAPELSTLESRLLVHHRRPAESSDEPVETWRHIADAAAHLFVDRSPGEVRVSEVAAAA
                     GVSTTTVYHHFGTVNAVAAAAWARHMPELESIASAPITAEEGPIRRIEQVLLRYVQMA
                     RANRGATEALAAQIVAEAAPSGERQWPRDTRAVVPLPGVLLPLIRELRAVGRLRRRME
                     TVRLARSLVHLTTMQALLFGDEPDERIVDETMSLIFDGALVAPSDG"
     gene            complement(2501..3775)
                     /locus_tag="VLR27_11335"
     CDS             complement(2501..3775)
                     /locus_tag="VLR27_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP04089.1"
                     /translation="MPDRRTALVDAGVELVLQQCFQDLLASVDTRSITGRAGVTTGSF
                     FHHFRNRAAFTDAVIDRLEELWAGSTEQSLSSIATFTEGLDFHGAAAAEWRRLESDER
                     NSGLQHLLLAVPRQPVSEGSTRTAADVLAARYRSLNEAVLPAYRRGLDAIGREVMPPF
                     HEHDLAVALTALGAGLRARYVADAGSVRPGLYADLVSALVIAVTRPVGEQVREAETLA
                     DLDRPVAVRAGKVGPERPGSTWRQIAEAAAPLFAERMVGEVKVAEIAEVAGVSASTVY
                     QRFGSVSAVAAAGWARHLPELEAIASKPLTTSEGPIVRMEQVLTRYIQLGRENRGALE
                     GQVLEGVARASSGANPTSTLMPLAGLLVPHIREMRARGVLRRRIDSEGLARSVLQLIA
                     MRVLTSPDEPEERIIDETLGMLLEGALARQVR"
     gene            complement(3881..>4354)
                     /locus_tag="VLR27_11340"
     CDS             complement(3881..>4354)
                     /locus_tag="VLR27_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HSP04090.1"
                     /translation="LIDHETFLSGTLHPDTVLETVDGTVIPIGVAERWLCDSRWQLLV
                     QDAAGEVLMLGREERYANRAIRRALAARDRGCAAPGCNAPVSHCDAHHVTWWDYEGES
                     NIDQMALLCRHHHRMVHAGALEIMMVNGLPIFLDRYGNELTDGGRRRPPPEVSAA"
BASE COUNT          612 a         1563 c         1589 g          590 t
ORIGIN      
        1 ccgaccgacg agatcgtcgg cggtgcgacc gcagatcatg gccagggcgt cgttcacctg
       61 cacgaagcga ccggcgagat cgaccacggc ggtggcgacg ggggaaccgt cgaagaccgc
      121 ccggaagagg ccctcggccg cccgctggct cgtgacgtcc tcgagggacg cgacggcgag
      181 gagcagccgg tgctcggcgt cgcgggtcgg cacgacgcgg aggcgcagcg agcgaccgtc
      241 gatcgcgagg tcggacacct cgccggcctc cgcacctgtg ccggcgtctg tgctcgtgct
      301 gtcgagggcg gcgcggatcc gggcctcgac ctcgtcccgg ccggccccga ccagctcgga
      361 gaagcggctg ttctcgaacg acacggtgcc gttgccgctg cgcacctgga gcccgaccgg
      421 tgcggcctcg gccagggagg acatggccat accggcctcg gcaccctcca gggcggcggc
      481 atcgaagtcg accagccagg cgtggacgag gaggccgaac ggcacgctgg cgctcacgtc
      541 gtgggcgacg atcatcagcg agctgccgac ctcggcggcg tggtcggggt ggacccgggc
      601 cacggtcgag cggctcccac cctcgtgggc ggcgcccgcc aggacgtcga gcacggccgg
      661 caggtcgtca gggtggatga aggtgatggc gcgcgccatc ggtgaccccg gaaggtgcaa
      721 gcggctggtg gtcgggtcgt accagcggtg gatcgaccgc ccggtgctgg catcgacgag
      781 gacggtgacg tcggccgatt ggtcgagcag gtggtgcagg acggtctcgg agagcacgcg
      841 ctcatcgtct acgactcctc caaccactcc tcccgtgacc cctccgagat ccatggcacc
      901 tcgatcggca cttccgcgcc ggaactggag ccctatccgt cgctcggggc caccagcgcg
      961 ccgtcgaaga tcagggacat ggtctcgtcg acgatgcgct cgtcgggctc gtcaccgaac
     1021 agcagcgcct gcatggtggt caggtgcacc agcgaccggg ccaggcggac ggtctccatg
     1081 cgccggcgga gtcgacccac ggcgcgcagc tcgcggatga gcgggagcag caccccgggc
     1141 aggggcacca cggcgcgggt gtcccgcggc cactgccgct cgccggacgg cgcagcctcg
     1201 gccacgatct gggcagccag cgcctcggtc gctccgcggt tcgcgcgggc catctgcacg
     1261 tagcggagca gcacctgctc gatgcgccgg atcggaccct cctcggccgt gatcggtgcc
     1321 gacgcgatcg actccagctc gggcatgtgc cgggcccagg ccgcagcggc cacggcgttc
     1381 acggtgccga agtggtggta cacggtggtc gtgctcacgc ctgccgcggc ggccacctcg
     1441 gacacgcgca cctcgccggg gctccggtcg acgaagaggt gggcggccgc atccgcgatg
     1501 tggcgccagg tctcgactgg ttcgtcggag ctctcggctg gacgacggtg gtggacgagg
     1561 agccgcgact ccagcgtcga cagctccggt gccgggtcgg cctccgaacg ctcgacgcgc
     1621 ggccgggtga tcccgaggag cacggcggac accgcgtcgg cgtagagacc ctcgcggacc
     1681 gactccgggt cggcgccgtg gcgcatctgg aggccttcgg cgagcgcggt catgatcacg
     1741 gtgagatcgc agctggtgaa tggcggcagc atctcgcggc cgatccgccg cacgccctcc
     1801 aggtaggtgg cctcgacgct gtcggtgagg cctcggtagg cgtcgtgcag gacatcgccg
     1861 gcggtgcggg cggtctccgc tccgagctcc tggcgccgca ccgaccacag caggtgctgc
     1921 agcgccgaca ggtcctcctc ggcgacgagg ccggcccact cctcggcggc ggcggggcgg
     1981 atgccgtcgt agccctcgcg gtcgttggac gcggtggccg caccttcgag acgatccacc
     2041 cgttccgccc accggctgac gaacgcgtcg gccacggcca gggcgaagtg cgagcggttg
     2101 cggaagtggt ggaagaacga accggtcgtc acccccgcct catccgcgat cgatcgcgtc
     2161 tccaccgccg ccaagagcgc ttggaactgc tgccgtccca gcaggcggat cccagcatcg
     2221 atcagttcgg cccgccggtc taccaccacg gcccggaacg tagccgcagg tcagcgcatc
     2281 ctccgggacc gagcgcgaac atgacaccca cgccagcaca tagcgcgcgc tacggcctgg
     2341 cgtaccaggc gcaatgtctt ggcaccccac atgccacgtc ggggtggttc actgaccacg
     2401 cagcgggaac ctgcgaacgg ccctcgggtc gttcctgtcc ctgtggcgag gcgtccggcg
     2461 caccccgggc gcctcgccct ggggtaacgg ggcacggtgc tcaccgcacc tggcgggcca
     2521 gggctccctc gagcagcatg ccgagcgtct cgtcgatgat ccgctcctcg ggctcatccg
     2581 gcgacgtcag cacccgcatg gcgatgagct ggagcaccga gcgggccagg ccctcgctgt
     2641 cgatgcgacg ccgcagcacg ccccgcgccc gcatctcgcg gatgtggggg accaggagcc
     2701 cggccagcgg catgagcgtc gaggtcgggt tggcgccgct gctggcacgg gcgacccctt
     2761 cgagcacctg cccttcgagc gcaccgcggt tctcccgacc cagctggatg tagcgggtga
     2821 ggacctgctc catgcggacg atcggtccct cgctggtggt cagcggcttc gacgcgatgg
     2881 cctccagctc ggggaggtgc cgggcccacc cggccgccgc cacggcgctg accgagccga
     2941 accgctggta cacggtgctg gcgctcaccc cggcgacctc ggcgatctcg gcgaccttga
     3001 cctcacccac catccgctcg gcgaacagag gcgcggcggc ctcggcgatc tggcgccacg
     3061 tgctgcccgg acgctcgggt ccgaccttcc ccgcgcggac cgccacgggg cggtccaggt
     3121 cggcgagcgt ctcggcctcg cgcacctgct cccccaccgg tcgggtcacg gcgatgacca
     3181 gcgccgacac caggtcggcg tacaagccgg ggcggaccga tcccgcgtcg gccacgtagc
     3241 gggcccggag tccggcgccg agggccgtga gcgccacggc gaggtcgtgc tcgtggaacg
     3301 gcggcatcac ctcccggccg atggcgtcga ggcctcgccg gtacgccgga agcacggcct
     3361 cgttcaggga ccggtagcgg gcggccagca cgtcggcggc cgtccgcgtg gagccctcgc
     3421 tcaccggttg ccggggcacg gccaggagga ggtgctggag gccgctgttg cgctcgtcgg
     3481 actcgagccg ccgccactcc gctgctgccg cgccgtggaa gtccaggccc tcggtgaacg
     3541 tggcgatcga agacagcgac tgctccgtcg accccgccca cagctcctcc aggcgatcga
     3601 tcaccgcgtc ggtgaacgcc gcccggttcc ggaagtggtg gaagaacgat ccggtggtga
     3661 cgccggccct cccggtgatc gaacgggtgt cgacgctggc cagcaggtcc tggaagcact
     3721 gctgcaggac cagctccacc ccggcgtcga ccagcgcggt gcggcggtca ggcatcggcg
     3781 gcgacgctaa cccagctgcg acacgcggca ccgaccccgc acgaggcggg gtgacccgga
     3841 agtggtcgcg ctggagcgtc ggctgtgccg tggccgcgtc ctacgcggcg gacacttcgg
     3901 gcggtggtcg tcgtcgtccg ccgtcggtca gctcgttgcc gtaccggtcg aggaagatcg
     3961 gaaggccgtt gaccatcatg atctcgagcg ctccggcatg gaccatgcgg tggtggtggc
     4021 ggcagagcag cgccatctgg tcgatgttgc tctcaccctc gtagtcccac cacgtgacgt
     4081 ggtgggcgtc gcagtggctg accggggcgt tgcagcccgg cgcggcgcag ccccggtcgc
     4141 gggcggccag cgcccgccgg atcgcccggt tggcgtagcg ctcctcccga ccgagcatca
     4201 gcacctcgcc ggccgcgtcc tgcaccagca gctgccagcg ggagtcgcac agccagcgct
     4261 cggcgacgcc gatggggatg acggttccgt cgacggtctc cagcacggtg tcggggtgca
     4321 gggtgccgga caggaacgtc tcgtggtcga tcag
//
LOCUS       DATISU010000284         3586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_576992, whole genome shotgun sequence.
ACCESSION   DATISU010000284 DATISU010000000
VERSION     DATISU010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576992"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VLR27_11345"
     CDS             <1..432
                     /locus_tag="VLR27_11345"
                     /inference="COORDINATES: protein motif:HMM:NF019044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04091.1"
                     /translation="AGQDTYGRFMQIRVFDCWIHEQDAREALDLPGHSSGHAVDVSVD
                     EHSGAVGFVIGKKAGATEGQSVRLHLTGDTERTIDVRVDGRAAVVDDLEDPTTTITIP
                     TLLWFRYAAGRRPGDASHPDVTIEGDQDLGQRVLANAAYTI"
     gene            complement(508..1254)
                     /locus_tag="VLR27_11350"
     CDS             complement(508..1254)
                     /locus_tag="VLR27_11350"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HSP04092.1"
                     /translation="MQLTLCDRCHFDAGDYRRHDLATAGLWLWAMADQYVEGVPSGVL
                     AREPQSATHAELRRLVSSLQVDAPDAEIVHRAIHHLHDVGRQLHAGGGGVPSQRGTVA
                     QLSASDGGVPKVPIDSVDVGLRGVLGDRQANRKHHGRPFQALCLWSAEVIEALQAEGH
                     PVHPGSTGENVTVAGIDWAELRPGARVLVGEVLCEISVWALPCRKIDRCFTGRSDRID
                     HATNPGWSRAYAWVLEPGAITTGDEVVVEP"
     gene            1365..2003
                     /locus_tag="VLR27_11355"
     CDS             1365..2003
                     /locus_tag="VLR27_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04093.1"
                     /translation="MTLETPDERWDAALAAVGAVAGDRVAAYADLVERHRAPGRYHHV
                     LDHTSGVVDAVLAIHGNGDDWATTVLAAWYHDVVYDPTAPAGANEGSSAVLARRALES
                     MGASLTAVGEVCRLICLTTDHDPGPGDRNGALLCDADLAVLASDEGGYVAYATAVRRE
                     YAHVDDDAWRVGRRTVLRSFLDRPRIFRTIVGRERWEARARINISSELASLG"
     gene            complement(2071..2319)
                     /locus_tag="VLR27_11360"
     CDS             complement(2071..2319)
                     /locus_tag="VLR27_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4031 domain-containing protein"
                     /protein_id="HSP04094.1"
                     /translation="MILVDQAIWPWRGHRWAHLVSDESYDELHEFADRLGIPRRAFQG
                     DHYDVPAHIRDEAIALGAEAVDARVLVRRLRDAGLRKR"
     gene            complement(2442..>3586)
                     /locus_tag="VLR27_11365"
     CDS             complement(2442..>3586)
                     /locus_tag="VLR27_11365"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HSP04095.1"
                     /translation="ADVAEVRSMLGGLAVPGWTLRAEQVLRGGLAATRAVVEVQDDAH
                     ARSAGDIIAIVDGSDLPERVRSRAVATFRALAEAEAELHGEHPDDVQLHEVGGHDAIV
                     DVVGTCAALELLGVDLVLAGPVATGVGTTRSAHGTIPVPAPAVVALLAGAPVTRVDVA
                     MELTTPTGAALLRALVTGWGPMPAMTIVGSGYGAGTRELAERVNAVQLVLGDATVADP
                     TGTGDGQPMVELAVNVDDATGEQLAHAIAQLMDAGAADAWITPILMKKGRPGHVVTVL
                     ADQALVPTLSTVLTTETGSLGVRSRPVDRWPTARHFDSVDVDGHPVRIKVTPARTKVE
                     HDDAAEVAKRTGRPLREVVSLAEEAARRRQRVPHPADPDFSPDEPA"
BASE COUNT          473 a         1327 c         1331 g          455 t
ORIGIN      
        1 gccggccagg acacctacgg ccgcttcatg cagatccggg tgttcgactg ctggatccac
       61 gagcaggacg cccgcgaggc gctggacctg ccgggccaca gcagcggcca cgccgtcgac
      121 gtcagcgtcg acgagcactc cggggccgtc ggcttcgtca tcggcaagaa ggcgggcgcc
      181 accgagggcc agtcggtgcg gctccacctc accggcgaca ccgagcggac catcgacgtc
      241 cgggtcgacg gacgcgcggc cgtcgtcgac gacctcgagg acccgaccac caccatcacg
      301 atcccgaccc tgttgtggtt ccgctacgcc gccggccggc gcccgggcga cgcgtcgcac
      361 cccgacgtca ccatcgaggg cgaccaggac ctcggtcagc gggtcctcgc caacgccgcc
      421 tacaccatct gaacgaacgg accgatcggt ccccacgttc caccgggtgg aacgaacgga
      481 ccgatcggcg cggtcgttcc cgcagcgcta cggctcgacg acgacctcgt cgccggtggt
      541 gatcgcgccc ggctcgagca cccaggcgta ggcgcgggac caccccgggt tggtggcgtg
      601 gtcgatgcgg tccgagcgac cggtgaagca gcggtcgatc ttgcgacacg gcagggccca
      661 caccgagatc tcgcacagca cctcgccgac gagcacgcgg gcgcccggcc gtagctcggc
      721 ccagtcgatg ccggccacgg tgacgttctc gcccgtggag ccggggtgca cggggtgccc
      781 ctcggcctgc agcgcctcga tcacctcggc ggaccacagg cacagcgcct ggaacggtcg
      841 accgtggtgc ttgcggttgg cctgccggtc gcccagcacg ccccgcaacc ccacgtcgac
      901 ggagtcgatc ggcaccttcg gcacgccccc gtccgaggcg gacagctgcg cgacggtgcc
      961 gcgctggctc ggcaccccac ccccgccggc gtgcagctgg cggccgacgt cgtggaggtg
     1021 gtggatcgcc cggtggacga tctcggcatc gggggcgtcg acctggagcg aggagacgag
     1081 ccgccgcagc tcggcatggg tggcggactg cggctcgcga gccaggacgc cggacggcac
     1141 gccctcgacg tactggtcgg ccatggccca cagccacagg ccggccgtgg ccaggtcgtg
     1201 gcgtcggtag tcgccggcat cgaagtggca ccggtcgcag agcgtgagct gcaccggcgg
     1261 atcctgccac ccgcagcgat cgcggacccc gagggttctg gctgcggtca cggaacgcgt
     1321 tccgcagtcg ctgccagaac gcgtcgtgct ggcagactcc cgccgtgacg ctggagacgc
     1381 cagacgagcg ctgggacgcc gccctcgccg ccgtcggcgc cgtggccggc gaccgggtcg
     1441 cggcctacgc cgacctggtc gagcgccacc gtgccccggg ccggtaccac cacgtgctgg
     1501 accacacgtc cggcgtcgtc gacgccgtgc tcgccatcca cggcaacggc gacgactggg
     1561 ccaccacggt gctggccgcc tggtaccacg acgtcgtcta cgacccgacc gctccggccg
     1621 gcgccaacga gggttcctct gccgtgctgg cccgccgggc gctcgagtcg atgggtgcgt
     1681 cgctcaccgc cgtcggcgag gtctgccggc tgatctgcct cacgaccgac cacgatcccg
     1741 gccccggcga ccgcaacggc gccctgctct gcgacgccga cctcgccgtg ctggccagcg
     1801 acgagggcgg ctacgtcgcc tacgccaccg ccgtgcgccg cgagtacgcc cacgtcgacg
     1861 acgatgcctg gcgggtgggc cgccggaccg tgctgcgctc gttcctcgac cgcccccgga
     1921 tcttccgcac catcgtcggc cgggagcgct gggaggcccg agcccgcatc aacatctcct
     1981 ccgagctcgc gtcgctgggc tgatcccgcc gcgacaccac aactgccagt tccaagaagg
     2041 cgttctggca gtggtggtgg agcgcgccga tcagcgcttg cggaggccgg cgtcccggag
     2101 gcgacggacc aggacgcggg cgtcgacggc ctcggcgccc agcgcgatcg cctcgtcgcg
     2161 gatgtgggcg ggcacgtcgt agtggtcgcc ctggaacgcc cggcggggga tgccgagccg
     2221 gtcggcgaac tcgtgcagct cgtcgtagga ctcgtccgac accaggtggg cccaccgatg
     2281 cccccgccac ggccagatcg cttggtcgac caggatcacc atcaggaccc ttccggagac
     2341 atcacgtcca cccgcagtcg agatggcggc tccgccgccg gaatgacacc gtgctcatgg
     2401 gtcgcctcgc ctccggctcg gctccgacga ggggagtcac gtcaggcggg ctcatcgggg
     2461 gagaagtcgg ggtcggccgg gtgcggcacg cgctggcgcc ggcgggcggc ctcctcggcc
     2521 agcgacacga cctcgcgcag cggccggccc gtgcgcttgg ccacctcggc ggcgtcgtcg
     2581 tgctcgacct tcgtccgggc cggcgtgacc ttgatgcgga ccgggtggcc gtcgacgtcg
     2641 acggagtcga agtgccgggc cgtgggccag cggtcgaccg ggcgcgagcg caccccgagc
     2701 gagccggtct cggtggtgag cacggtgctg agcgtcggca cgagggcctg gtcggcgagc
     2761 accgtcacca cgtggccggg tcgccccttc ttcatgagga tcggggtgat ccaggcgtcg
     2821 gcggcgccgg cgtccatgag ctgggcgatg gcgtgggcca gctgctcgcc ggtggcgtcg
     2881 tcgacgttga cggcgagctc gaccatgggc tggccgtcgc cggtgccggt ggggtcggcc
     2941 acggtggcgt cgccgagcac cagctgcacg gcgttgacgc gctcggccag ctcccgggtg
     3001 ccggcgccgt agccggagcc gacgatggtc atggccggca tcggacccca tccggtgacc
     3061 agggcccgga gcagcgccgc gccggtgggg gtggtgagct ccatggcgac gtcgacccgg
     3121 gtgaccgggg cgccggcgag cagggccacc accgccggtg cggggacggg gatcgtgccg
     3181 tgggcggagc gggtcgtgcc gacgccggtg gcgaccggcc cggcgagcac caggtcgacg
     3241 ccgagcagct cgagggcggc gcacgtgccg acgacgtcga cgatggcgtc gtggccgccg
     3301 acctcgtgga gctggacgtc gtcggggtgc tcgccgtgca gctcggcctc ggcctcggcg
     3361 agggcccgga acgtcgccac cgcccggctg cgcacgcgct cgggcaggtc gctgccgtcg
     3421 acgatggcga tgatgtcgcc ggcagaacgg gcgtgggcgt cgtcctggac ctcgaccacg
     3481 gcgcgggtgg cggcgagccc gccccgcagc acctgctcgg cgcgcagggt ccagcccggc
     3541 acggcgaggc cgccgagcat cgagcggacc tccgccacgt cggcgc
//
LOCUS       DATISU010000285        13357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_437782, whole genome shotgun sequence.
ACCESSION   DATISU010000285 DATISU010000000
VERSION     DATISU010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 13357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13357
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437782"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /gene="rsmH"
                     /locus_tag="VLR27_11370"
     CDS             <1..1056
                     /gene="rsmH"
                     /locus_tag="VLR27_11370"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HSP04096.1"
                     /translation="VPDRGIHPGGPSAAEGLPVSDADDSTRTFRHVPVLRDEVVAAFA
                     PVPPGWVVDGTLGGAGHATAILDAHPHLSVLGLDRDPLAVQVATERLARFGDRAHVVH
                     TRFDRIADAMSLLRRTRTTADTTEEEIPTGLSGILLDLGVSSPQLDRGDRGFSYRNSG
                     PLDMRMDPTTGRSASDVVNEADHGELVRILRRFGDERHADRVARAIIEARPVTTTAQL
                     ADVVRDAIPAAARRTGGHPATRTFQALRIEVNDELAQLEAVLGDALDLLVPGGRMAVI
                     TYHSGEDRIVARRFRDAAADDCVCPPGSGTCRCGGAPSVKLVPRKAITASEDEIAANP
                     RARSARLRVAEQLGEAS"
     gene            1053..1634
                     /locus_tag="VLR27_11375"
     CDS             1053..1634
                     /locus_tag="VLR27_11375"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HSP04097.1"
                     /translation="MTAVVPARPSRPTHDRGHVAPARRPEHRRGQHLRPVERPRPKRS
                     PAVPALVGVGMVIVALLGLAGMHAVLIGGQVRLDAMRSDVAAETESIRRLELRVAELE
                     SPDRVLDVARDRLGMVLPTEVGYLLPVGLDGVPAEPVRVEPAPAEAEAEGDAETEAET
                     GAEVETETDVEADGAGDSSELAADQVPEGGLEE"
     gene            1631..3697
                     /locus_tag="VLR27_11380"
     CDS             1631..3697
                     /locus_tag="VLR27_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HSP04098.1"
                     /translation="MSWGPIDPDWGEGWEDFDIDPFAEEPRPHGRGGARPPRTATVHR
                     LPRPRPGRRGVPLDRSLTAPRPTAPAPRHPPVARRAPRTHELPDPAWPAPTAAHTVHE
                     VDTRHARPAHGGGGGGGRPPRQPASRPAARPERRSLALKALLAVLLLAVVGKLVHVQV
                     LEPDRFVEVGQSQRLRTVQLEADRGRILDREGHDLALSVLRPTVFADPSQIEDPLATA
                     RRLAPVLGMEVSDLTDLLDGDNRFAYLSRRIDDDVAARVVELELDGVHLLEEPARFTP
                     SEELARAIIGRVAPDQTGATGMELLYDEVLTGTPGEMTYERARDGGTIAAAGQSRVAP
                     RAGSDVVLTLDRSLQYETERVLAEQVDALGAKGGTVIISQPESGEILALANITRNTDD
                     EIITAGANRAVIDVFEPGSVNKVITIAAALEEGLVSPGTELVVPDSLQVSDHRFSDDH
                     EHPTMPMTVTQILAESSNVGTIMLAQDLGKERIDQYLRSFGFGSKSALGFPNESPGLL
                     LPTDSWSGTSIGSIPIGQGIAVTAMQMLDAYNVIANDGIHIPPRLVAATVDADGTRTP
                     IESGEPRRVVSAETAQQVREMLVEVVDNGTGENAQVAGYSVGGKTGTARKPQDTGTYR
                     DAAGNYHYVAAFTGMVPAEDPALSIIVAIDEPTATIYGGSAAAPVFADLAKFALNRFR
                     IPPAGS"
     gene            3697..5142
                     /locus_tag="VLR27_11385"
     CDS             3697..5142
                     /locus_tag="VLR27_11385"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HSP04099.1"
                     /translation="MLLSRAATGLADAGIDHEVRGDVEISGVVHRTDTVLAGVLFCCV
                     PGSRVDGHDLAADVVDAGAVALLVERPLTVDVPQVIVPSVRAAMGPVAARFWGDPSRD
                     LDVIGITGTNGKTTTVAILRAVLDAAGRRCEVVGTLTSRPGWPPTTPDAPELQAQLAT
                     WRDEGVDTVAMEVSSHALAMGRVDGTWFTATGFTTLGHDHLDLHGDIESYFEAKASLF
                     RPDRTDRAAIRADDEWGARLLERVEGTAVEVHPYRLEDAADLEPFGTGSRFTLRGVPV
                     ELPLAGRHNVANALCAAAIADWLGIDPDTVARGLASVPTVAGRFERVDEGQAFTLAVD
                     YAHTPDALQTVLDAARERADVDGGRVLVVFGCGGDKDRAKRPEMGRIAAASADVVVVT
                     SDNPRSEDPAAIAGEIVAGIPAQEPGQADVRTILDRAEAIHHAAREAREHDVVVIAGK
                     GHETTQTFADRVVDFDDRVVAATALREVLGR"
     gene            5139..6170
                     /gene="mraY"
                     /locus_tag="VLR27_11390"
     CDS             5139..6170
                     /gene="mraY"
                     /locus_tag="VLR27_11390"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HSP04100.1"
                     /translation="MIPLLIAAGVSLMVSLVGTRYLIDWLTAHQIGQPIHEDVEGHVT
                     KAGTPTMGGVAFVVAALAGYLAAHVRSGLVFTRTGLIVMGTILGAGIVGALDDWIKVS
                     RERNLGLSSRAKMLGLLVVAIGFVVATLTFTDVHTTISFTRFDSPGFDIPDWTWAIWA
                     MLLLAGTTNAVNLTDGLDGLAAGSSIFAFITFVVVGFWAFRHPEVYRVEHALDLAVVA
                     AAMLGGVTGFLWWNANPARIFMGDTGALAIGAALACLALTTNTQLLLPVIGFLFVLET
                     VSVMLQVTRFRLTGKRFFRMAPIHHHFELGGWPETTVIIRFWIMAGLATALALGMFYA
                     DYIGTGGLD"
     gene            6170..7519
                     /gene="murD"
                     /locus_tag="VLR27_11395"
     CDS             6170..7519
                     /gene="murD"
                     /locus_tag="VLR27_11395"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HSP04101.1"
                     /translation="MPPRRLDAVPDRVLLVGFGLTNQAVAAALLARGHHVTVTDDAPS
                     DATRAAAEQIGVALLAAPDEGTLEALVADADLVVPSPGLPDAHPVFAASAAHGTTSVT
                     ELDLAAVWDQRPIVAITGTNGKTTVTTMVTAMLDTAGIACVDAGNNDLPLVAAIDDPA
                     PEWFVVEASSFRLGRTHGWAPRVATWLNLAPDHLDVHATHADYEAAKARIWSAQASTD
                     VAVGNLDDPVVARHLAAAPARHVSWSTSDPAADYHLAGGELIGPDGVIVAATELPRAL
                     DHDISNALAAAATAEAAGCPRHAVREVLVGFGPLPHRVAPVATIDGATFYDDSKATVP
                     HATLAAVRGFDSVVLIAGGRNKGLDLGTLAEGVDHVRAVVAIGEAAPDVQAAFDGLRP
                     VELAGSMSEAVATAARLAQPGDAVLLSPACASFDWYPSYAARGDDFAAAVHALEETS"
     gene            7516..8775
                     /gene="ftsW"
                     /locus_tag="VLR27_11400"
     CDS             7516..8775
                     /gene="ftsW"
                     /locus_tag="VLR27_11400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HSP04102.1"
                     /translation="MTAIGLRRTRHPRLRLVPPPRSTDVGTAAQPASGATASQRLGLS
                     LVVVVALLSLLGSVMILSASSVWAVEEVGSAFAVFIRQCMWLVIASVALFIGARVDYH
                     RWARLVPVLVLGSFGLLVAVLIPGIGMSVNGAQRWIGFGFLQLQPAEPAKFALIVFCA
                     ALVARNSHRVHQLGVVLRPALVVLGMFALLLMKQPNLGTTMILCAIVGGMLLWAGVPM
                     RILGVLGAAAVVLGTTFALSADYRRARVTGFLDPWENAGDSGYQTIQSLIGVASGGIS
                     GTGLGASRAKWGFLPEAHTDFVFAIIGEELGLIGCLFVVALFATLIGLGLRIAQRASD
                     PFGAMLAVGVTTWFAVQGFVNVGAVLSLLPITGVPLPFVSFGGSSLVATMLAAGVLVN
                     VAFQGEEPDNPVRARIAARRARNAVRG"
     gene            8785..9855
                     /gene="murG"
                     /locus_tag="VLR27_11405"
     CDS             8785..9855
                     /gene="murG"
                     /locus_tag="VLR27_11405"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HSP04103.1"
                     /translation="MKILVTGGGTAGHVLPALAIATALRDRGHDVHLVGSERGIEARL
                     VPEAGFAQTLLPGRGIARSVSPSALRANVGAVVGLVRAVLMAIGLVRRHRPAAVVAVG
                     GYASVATGAAAVLWRVPIIVHEQNAVPGAANRLLARFAARSATSFPDTDLPRAEVVGN
                     PVRAELFDARHEDRSDGRTQLLVFGGSLGALSINRATIAAADRWRDRGDLAVHHVIGR
                     RDWPTLDRPTETGALAYEAVEYEDDMPAQLARADLVVCRGGSGTLFELAATGRPAVIV
                     PSLVTTGGQQVANAERVAATGGAVVIRDDELDGDRLVAEVDALLADPDRLRTMSERVS
                     TLATPDAADRVAALVEEHARAR"
     gene            9845..11251
                     /gene="murC"
                     /locus_tag="VLR27_11410"
     CDS             9845..11251
                     /gene="murC"
                     /locus_tag="VLR27_11410"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HSP04104.1"
                     /translation="MPADRPQQSIDLSTPRRLHVVNVGGAGMSAIATELAQMGHAISG
                     VDDKDTPFLAPLRALGVDVTVGSQGLADPAAADAVVTSTATPADHPDVVAAEAAGVPV
                     LHRGDALASICAERDVLAVAGTHGKSTTTGMVATILEGVGDGAGYLAGTRITGLDRNA
                     DWRTGPGFVVEADESDGTFLRVGATDALVTNVEADHLSYWGTEDALVEGFRTLVGGLD
                     GTAVLCLDDPGAAALAPFAGRTVTYGTDDAADLRITDVGPAGIGVRFTLHHDGDAVEV
                     EVPAAPGLHNARNAAGALAACLVRGVPLADGARALLGFAGVARRFEQRGVADGVTFVD
                     SYDHLPTEVAAALAAARTGPFDRIVCVFQPHRYTRTRDLAHTFADAFVDADVLGVTEL
                     YPAGEAPIEGVSGALVRDAVVAAHPDADVAFLPDLPAAEAWLLDVLRPGDLCLTLNAG
                     DLTATPDRVLAALRERGS"
     gene            11248..12171
                     /gene="murB"
                     /locus_tag="VLR27_11415"
     CDS             11248..12171
                     /gene="murB"
                     /locus_tag="VLR27_11415"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HSP04105.1"
                     /translation="MSPDPLTIAGEVLGDLAQPMVPLGPFTTYKVGGPAALFVEVTDD
                     DVLAAVGAAVAASGVAVLVVGNGSNMLVSDAGFPGLAIRLGDAYATVEVDGTEVTAGG
                     AALLPVVARQSVKAGLTGFEWAVGVPGSIGGAVRMNAGGHGSDMAASLLGVRVFDCRT
                     GEDANVPASALELGYRRSAITSDQVVLRATLGLALDEAGTGSDTLSEIVRWRRANQPG
                     GQNAGSVFTNPDGDSAGRLVDAAGCKGLRRGTAEVSPKHANFIQADAGGLADDVFAVM
                     VAVRDAVEADSGVRLHPETRLVGFDEAEVTW"
     gene            12168..12992
                     /locus_tag="VLR27_11420"
     CDS             12168..12992
                     /locus_tag="VLR27_11420"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HSP04106.1"
                     /translation="MSGPRVLDRDAEQAAPPLAAGVDPRFRARRIAVRKDAGRKRLKR
                     LLLLVGVAVLALAAVVVLRSPVLDVDEVVVVGTSRLDPATVRSEAGIGQGSPLLLADL
                     GAAADRIESMPWVAEAEVTRDLPGRVDIAIREREPVAVVSGAGTAVLVDVDGRVLEVA
                     PPVAGAFVQVVAAEAPPEPGDVVDPELLTAVGLAGRLRVNPAGAVAAVHLEPSLQLHL
                     AEGGVVDLGDTSAIDAKVEAFRTVHARVDRSCLATIDLTVPTHPVLTRDQGCGGGG"
BASE COUNT         1646 a         5480 c         4443 g         1788 t
ORIGIN      
        1 gtgcccgacc ggggcataca tcccggcggg ccttcggcgg ccgaggggct gccggtgagc
       61 gacgcagacg actccacccg gaccttccgc cacgtccccg tcctgcggga cgaggtcgtc
      121 gccgccttcg ccccggtccc gccgggctgg gtggtcgacg gcacgctcgg cggagccggc
      181 cacgccacgg cgatcctcga cgcccacccc cacctctcgg tgctgggact ggaccgagat
      241 ccgctcgccg tgcaggtggc caccgagcgg ctcgcccgct tcggggaccg ggcccacgtg
      301 gtgcacaccc gcttcgaccg gatcgccgat gccatgagcc tcctgcgccg cacccgcacc
      361 accgccgaca ccacggagga ggagatcccc accgggctct ccggcatcct cctggacctc
      421 ggcgtctcct caccccagct cgaccgcggc gaccgcggct tcagctaccg caacagcggc
      481 ccgctcgaca tgcgcatgga ccccaccacg ggacgctccg cctccgacgt ggtcaacgag
      541 gccgaccacg gggagctggt ccgcatcctg cgccgcttcg gcgacgagcg ccacgccgac
      601 cgcgtcgccc gcgccatcat cgaggcccgc ccggtcacca ccacggccca gctggccgac
      661 gtcgtgcgcg acgccatccc ggccgcagcc cgccgcaccg gcggccaccc cgccacccgc
      721 acgttccagg cgctgcgcat cgaggtgaac gacgagctgg cccagctgga ggccgtgctc
      781 ggcgacgccc tcgacctcct ggtccccggc ggtcgcatgg ccgtcatcac ctaccactcc
      841 ggggaggacc gcatcgtggc ccgccgcttc cgcgacgccg cagccgacga ctgcgtctgc
      901 cccccgggca gcggcacgtg ccgctgcggc ggtgcgccga gcgtgaagct ggtgccccgc
      961 aaggcgatca ccgccagcga ggacgagatc gccgccaacc cccgtgcccg cagcgcccgc
     1021 ctccgggtgg ccgagcagct cggtgaggcc tcgtgacggc ggtcgtcccg gcgcgcccgt
     1081 cgcgccccac ccacgaccgc ggccacgtcg ccccggcccg ccgccccgag caccgccggg
     1141 gccagcacct gcgccccgtc gagcgcccgc gcccgaagcg ctcgcccgcc gtccccgccc
     1201 tcgtcggcgt ggggatggtc atcgtcgccc tgctcggcct cgccggcatg cacgccgtgc
     1261 tcatcggcgg ccaggtccgc ctcgacgcca tgcgcagcga cgtcgccgcc gagaccgagt
     1321 ccatccgccg cctcgagctg cgggtcgccg agctcgagtc acccgaccgc gtcctcgacg
     1381 tcgcccgcga ccgcctcggg atggtcctgc ccaccgaggt cggctacctc ctccccgtcg
     1441 gcctcgacgg cgtccccgcc gagcccgtcc gcgtcgagcc cgctcccgcc gaagccgaag
     1501 ccgaaggcga cgcagaaacc gaggccgaga ccggggccga ggtcgagacc gagaccgacg
     1561 tcgaagccga cggtgccggg gactcgtccg agctcgccgc cgaccaggtg cccgagggag
     1621 ggctcgaaga gtgagctggg gtccgatcga ccccgactgg ggcgagggtt gggaggactt
     1681 cgacatcgat cccttcgccg aggagccacg gccccacggc cgtggtggcg ctcgcccgcc
     1741 ccgcaccgcc accgtccacc ggttgccccg cccgcgaccc ggtcgccggg gggtccccct
     1801 cgaccggtcc ctcaccgcgc cgcggccgac cgcacccgcg ccccggcacc cacccgtggc
     1861 ccggcgcgcg ccgcgcaccc atgagctccc cgacccggcg tggccggcgc ccacggccgc
     1921 ccacacggtg cacgaggtcg acacccgcca cgcccgcccc gcccacggcg gcggtggcgg
     1981 tggtgggcga cccccgcgcc agccggcgtc gcgcccggcg gcccggcccg agcgacggag
     2041 cctggccctc aaggccctcc tcgccgtcct gctgctggcc gtggtcggca agctcgtgca
     2101 cgtccaggtg ctcgagcccg accgcttcgt cgaggtcggc cagtcccagc gcctgcgcac
     2161 ggtgcagctc gaagccgacc gcggtcgcat cctcgaccgg gagggccacg acctggccct
     2221 gtcggtgctg cgccccacgg tgttcgccga ccccagccag atcgaggacc ccctcgccac
     2281 cgcacgccgg ctcgcgccgg tgctcggcat ggaggtgtcc gacctcaccg accttctcga
     2341 cggggacaac cgcttcgcct acctgtcccg ccgcatcgac gacgacgtgg ccgcacgggt
     2401 cgtggagctc gagctcgacg gcgtccacct gttggaggag cccgcccggt tcaccccgtc
     2461 ggaggagctg gcccgggcca tcatcggccg cgtggccccg gaccagaccg gcgcgaccgg
     2521 catggagctg ctctacgacg aggtgctcac cggcacgccg ggcgagatga cctacgagcg
     2581 ggcccgcgac ggcggcacca tcgccgccgc cgggcagtcc cgcgtcgctc cccgagccgg
     2641 cagcgacgtg gtcctcaccc tggaccgcag cctgcagtac gagaccgagc gggtcctggc
     2701 cgagcaggtc gacgccctcg gcgccaaggg tggcaccgtc atcatcagcc agcccgagag
     2761 cggcgagatc ctggccctgg ccaacatcac ccggaacacc gacgacgaga tcatcacggc
     2821 cggcgccaac cgcgcggtga tcgacgtctt cgagcccggg tcggtgaaca aggtcatcac
     2881 catcgccgcc gccctcgagg agggcctggt cagccccggc accgagctgg tcgtgccgga
     2941 cagcctccag gtgtcggacc accgcttctc cgacgaccac gagcacccga cgatgccgat
     3001 gacggtcacc cagatcctgg ccgagtcctc aaacgtcggc acgatcatgc tcgcccagga
     3061 cctcggcaag gagcggatcg accagtacct gcgcagcttc gggttcggtt cgaagtccgc
     3121 cctcggattc ccgaacgagt cgcccggcct gctcctgccc accgactcct ggtcgggcac
     3181 gtcgatcggc tcgatcccca tcggccaggg catcgccgtg accgccatgc agatgctgga
     3241 cgcgtacaac gtgatcgcca acgacgggat ccacatcccg ccccgcctgg tcgccgccac
     3301 ggtcgacgct gacggcacac ggacgccgat cgagtccggg gagccccgcc gggtcgtgtc
     3361 ggccgagacc gcccagcagg tgcgggagat gctcgtcgag gtggtcgaca acggcaccgg
     3421 ggagaacgcc caggtggccg gctactcggt gggcggcaag acgggcaccg cccgcaagcc
     3481 ccaggacacc ggcacctacc gagatgcggc cgggaactac cactacgtgg cggccttcac
     3541 cggcatggtt cccgctgagg atcccgcgtt gtccatcatc gttgccatcg acgaacccac
     3601 cgccaccatc tacggcggct ccgccgccgc tcccgtcttc gccgacctgg cgaagttcgc
     3661 cctgaaccgc ttccgcatcc cgccggcagg ttcgtgatgc tgctgtcgcg cgcggccacg
     3721 ggcctcgccg acgccggcat cgaccacgag gtgcgcggcg acgtcgagat ctcgggcgtc
     3781 gtccaccgca ccgacacggt cctcgccggc gtcctcttct gctgcgtgcc gggcagccgc
     3841 gtcgacggcc acgacctggc tgccgacgtg gtcgatgccg gcgccgtcgc cctcctcgtc
     3901 gagcgccccc tcaccgtcga cgtcccccag gtgatcgtgc cgtccgtccg agctgccatg
     3961 gggccggtgg ccgcccgctt ctggggcgac cccagccggg acctcgacgt catcggcatc
     4021 accggcacca acggcaagac cacgacggtg gccatcctgc gcgccgtgct cgacgccgcc
     4081 ggtcgccggt gcgaggtcgt gggcacgctg acgtcccgtc ccggctggcc gccgaccacg
     4141 cccgacgccc ccgagctcca ggcccagctg gccacctggc gcgacgaggg cgtggacacc
     4201 gtggccatgg aggtctcctc ccacgccctc gccatgggcc gcgtcgacgg gacctggttc
     4261 accgccaccg ggttcacgac gctgggccac gaccacctcg acctgcacgg cgacatcgag
     4321 tcctacttcg aggccaaggc gtcgctgttc cgacccgacc gcaccgaccg ggccgccatc
     4381 cgcgccgacg acgagtgggg cgcccggctg ctcgaacggg tcgaggggac cgcggtggag
     4441 gtccacccct accgcctcga ggacgccgcc gatctcgaac cgttcggcac cggctcccgg
     4501 ttcacgctgc gcggggtccc cgtggagctc cccttggccg gccggcacaa cgtggccaac
     4561 gccctgtgcg cggccgccat cgccgactgg ctcggcatcg accccgacac cgtggcccgg
     4621 ggtctggcgt ccgtccccac cgtcgccggc cgcttcgagc gggtcgacga ggggcaggcc
     4681 ttcacgctgg cggtcgacta cgcccacacc cccgacgccc tccagacggt gctcgacgcc
     4741 gcgcgggagc gagccgacgt cgacggcgga cgcgtgctgg tggtcttcgg ctgcggcggg
     4801 gacaaggacc gggccaagcg gccggagatg ggacgcatcg ccgccgcatc tgctgacgtg
     4861 gtggtcgtga ccagcgacaa cccccggtcc gaggatcccg ccgccatcgc cggcgagatc
     4921 gtcgccggga tccccgcgca ggagccgggt caggccgacg tccggaccat cctggaccgt
     4981 gcggaggcca tccaccacgc agcgcgcgag gcgcgggagc atgacgtcgt ggtgatcgcc
     5041 ggcaagggtc acgagacgac ccagacgttc gccgaccggg tggtggactt cgatgatcga
     5101 gtcgtggccg cgaccgccct ccgcgaggtg ctcggccggt gatccccctc ctcatcgccg
     5161 ccggcgtgtc cctgatggtc tccctcgtcg gcacccgcta cctcatcgac tggctcacgg
     5221 cgcaccagat cggccagccc atccacgaag acgtcgaggg ccacgtcacc aaggcgggca
     5281 cgccgacgat gggcggggtc gccttcgtcg tcgccgccct cgccggctac ctcgccgccc
     5341 acgtccggtc cggcctggtc ttcacccgca ccggcctcat cgtgatgggc accatcctcg
     5401 gggccggcat cgtcggggcc ctggacgact ggatcaaggt ctcgcgcgag cgcaacctcg
     5461 ggctgtcgag ccgggccaag atgctcggcc tgctcgtggt cgccatcggc ttcgtcgtgg
     5521 cgacgctcac gttcaccgac gtgcacacca cgatctcctt cacccggttc gactcgcccg
     5581 gcttcgacat ccccgactgg acctgggcga tctgggcgat gctcctgctc gccggcacga
     5641 ccaacgccgt caacctcacc gacggcctcg acggcctcgc cgcggggtcg tcgatcttcg
     5701 ccttcatcac cttcgtcgtc gtgggcttct gggcgttccg gcaccccgag gtgtaccggg
     5761 tcgagcacgc cctcgacctc gccgtggtcg cggccgccat gctcgggggt gtcaccggct
     5821 tcctctggtg gaacgccaac ccggcgcgca tcttcatggg ggacaccggc gccctggcca
     5881 tcggcgccgc gctcgcctgc ctggcgctga ccaccaacac ccagctgctg ctgccggtca
     5941 tcgggttcct gttcgtgctc gagaccgtct cggtgatgct gcaggtcacc cgcttccggc
     6001 tcaccggcaa gcgcttcttc cggatggccc ccatccacca ccacttcgag ctcggcggct
     6061 ggcccgagac cacggtcatc atccgcttct ggatcatggc cggcctcgcc accgcgctcg
     6121 cgctcggcat gttctacgcc gactacatcg gcaccggggg cctggactga tgccgccccg
     6181 acgcctcgac gccgtgcccg accgggtgct cctggtgggc ttcggcctga ccaaccaggc
     6241 cgtggctgcc gcgctgctcg cccgcggcca ccacgtgacc gtgaccgacg acgcaccctc
     6301 ggacgcgacc cgcgccgccg ccgagcagat cggggtggcg ctgctcgccg cccccgacga
     6361 gggcaccctc gaggctctgg tcgccgacgc cgacctcgtc gtgccctcgc ccgggctccc
     6421 cgacgcccac ccggtgttcg ccgccagcgc cgcgcacggc accacctcgg tcaccgagct
     6481 cgacctggcc gcggtgtggg accagcgccc gatcgtggcc atcaccggca cgaacggcaa
     6541 gaccacggtg accaccatgg tcaccgccat gctcgacacc gccggcatcg cgtgcgtcga
     6601 cgccggcaac aacgacctgc ccctggtcgc ggccatcgac gaccccgccc ccgagtggtt
     6661 cgtggtcgaa gcgtcctcgt tccgcctcgg tcgcacccac gggtgggccc cccgggtcgc
     6721 gacctggctc aacctcgccc ccgaccacct cgacgtgcac gccacccacg ccgactacga
     6781 ggcggccaag gcccgcatct ggtccgccca ggcgagcacc gacgtcgccg tcggcaacct
     6841 ggacgatccc gtcgtcgccc ggcacctcgc ggccgcaccc gcccggcacg tgtcctggtc
     6901 cacctccgac cctgctgccg actaccacct ggcgggcggg gagctcatcg ggccggacgg
     6961 ggtcatcgtc gctgccacgg agctgccccg agcgctggac cacgacatct ccaacgcgct
     7021 ggccgccgcc gccaccgccg aggccgccgg ctgcccgcgc cacgcggtcc gcgaggtcct
     7081 cgtcggcttc ggtccgctcc cgcaccgggt cgcgccggtc gccaccatcg acggcgccac
     7141 cttctacgac gactccaagg ccaccgtccc ccacgccacc ctcgcggccg tccggggctt
     7201 cgactccgtc gtcctcatcg ccggcggccg caacaagggc ctcgacctcg gcaccctcgc
     7261 cgagggggtc gaccacgtcc gggccgtggt ggccatcggc gaggctgcgc ccgacgtcca
     7321 ggccgccttc gacgggctcc gcccggtcga gctcgccggc tccatgtccg aggcggtcgc
     7381 caccgccgcc cgcctcgccc agccgggcga cgccgtactg ctctcacctg cgtgcgcgtc
     7441 gttcgactgg tacccgagct acgccgcgcg cggcgacgac ttcgccgccg ccgtccacgc
     7501 cctcgaggag acctcatgac cgcgatcggc ctgcgccgga cccgccaccc gcgactgcga
     7561 ctggtgcccc cgccgcggag caccgacgtc ggcaccgccg cccagcccgc ctccggtgcc
     7621 acggcatcgc agcgcctggg cctgtccctg gtggtggtcg tcgcgctgct gagcctgctc
     7681 gggtccgtga tgatcctgtc ggcgtcgtcg gtgtgggcgg tggaggaggt cggctccgcg
     7741 ttcgccgtgt tcatccgcca gtgcatgtgg ctcgtcatcg cctcggtcgc cctcttcatc
     7801 ggcgcccgcg tcgactacca ccgctgggcc cggctggtgc cggtgctcgt gctcggctcg
     7861 ttcggcctgc tggtcgccgt gctgatcccc ggcatcggga tgagcgtgaa cggggcccag
     7921 cgctggatcg gcttcggctt cctccagctc cagcccgcag aaccagccaa gttcgccctc
     7981 atcgtcttct gcgccgcgct cgtcgcccgc aactcccacc gggtccacca gctcggcgtg
     8041 gtgctccggc ccgccctcgt cgtcctcggc atgttcgcgc tgctgctcat gaagcagccc
     8101 aacctgggca ccaccatgat cctgtgcgcc atcgtcggcg gcatgctgct gtgggccggc
     8161 gtgcccatgc ggatcctcgg cgtgctgggc gccgccgccg tggtcctcgg caccaccttc
     8221 gccctgtcgg cggactaccg ccgggcccgg gtcaccgggt tcctcgaccc gtgggagaac
     8281 gcgggggact ccggctacca gaccatccag tcgctgatcg gcgtcgcgtc cggcggcatc
     8341 tccggcaccg gcctcggcgc cagccgggcg aagtgggggt tcctccccga ggcccacacc
     8401 gacttcgtct tcgccatcat cggcgaggag ctcgggctca tcggctgcct gttcgtcgtc
     8461 gccctcttcg ccaccctcat cggcctcggc ctgcgcatcg cccagcgggc ctcggacccc
     8521 ttcggcgcca tgctcgccgt cggcgtcacg acctggttcg cggtccaggg cttcgtgaac
     8581 gtgggcgccg tcctcagcct cctgccgatc accggcgtgc ccctgccctt cgtctccttc
     8641 ggcgggtcct ccctcgtggc caccatgctc gccgccggcg tgctcgtgaa cgtcgccttc
     8701 cagggcgagg agcccgacaa ccccgtccgg gcccgcatcg ccgcccgccg ggctcgcaac
     8761 gccgtccgcg ggtagggagc gccggtgaag atcctcgtca ccggcggcgg caccgccggc
     8821 cacgtcctgc ccgccctcgc cattgccacc gccctgcgcg accgcggcca cgacgtgcac
     8881 ctcgtcgggt ccgagcgcgg catcgaggcg cgcctggtgc ccgaggccgg attcgcccag
     8941 accctcctgc ccgggcgcgg catcgcccgc agcgtctcgc cttccgccct gcgggccaac
     9001 gtcggcgccg tggtgggcct cgtgcgggcc gtcctcatgg ccatcggcct cgtccgccgc
     9061 caccggccgg ccgcggtcgt cgccgtcggc ggctacgcca gcgtggccac cggggccgcc
     9121 gccgtgctgt ggcgggtccc catcatcgtg cacgagcaga acgccgtgcc cggcgccgcc
     9181 aaccgcctgc tcgcccgctt cgccgcccgc agcgccacgt cgttccccga caccgacctg
     9241 ccccgggccg aggtcgtcgg caacccggtg cgggccgagc tgttcgacgc ccggcacgag
     9301 gaccggtccg acggtcggac ccagctcctc gtcttcggtg gctccctcgg tgccctctcc
     9361 atcaaccggg cgaccatcgc cgccgccgat cgctggcgcg accgcggcga cctcgccgtg
     9421 caccacgtca tcggccggcg ggactggccc acgctcgacc gtcccaccga aacgggcgcg
     9481 ctggcgtacg aggccgtcga gtacgaggac gacatgccgg cgcagctggc ccgcgccgac
     9541 ctcgtcgtct gccggggcgg gtccggcacg ctgttcgagc tggctgccac cggccgcccg
     9601 gcggtcatcg tcccctcgct ggtcaccacc ggcggccagc aggtcgccaa cgccgagcgg
     9661 gtggccgcca ccggcggcgc cgtggtgatc cgcgacgacg agctcgacgg tgaccggctg
     9721 gtcgccgagg tggacgcact gctcgccgac cccgatcgcc tccgcaccat gtcggaacgg
     9781 gtgtcgaccc tcgccacccc cgacgccgcc gaccgggttg ccgccctcgt cgaggagcac
     9841 gcccgtgccc gctgatcggc cccagcagtc gatcgacctg agcacccccc gccgcctcca
     9901 cgtggtgaac gtcggcgggg caggcatgag cgcgatcgcc accgagctcg cccagatggg
     9961 ccacgccatc tcgggcgtgg acgacaagga cacgccgttc ctggctccgc tgcgagccct
    10021 cggcgtggac gtcaccgtcg ggagccaggg cctcgccgac cccgcggccg ccgacgccgt
    10081 cgtcacctcc acggccacac ccgccgacca ccccgacgtg gtcgcggccg aggcggcggg
    10141 cgtgcccgtg ctgcaccgcg gggacgcgct ggcgtccatc tgcgccgagc gcgacgtgct
    10201 cgccgtggcc ggcacccacg ggaagtccac gaccacgggc atggtcgcca ccatcctcga
    10261 aggcgtcggc gacggcgccg gctacctcgc cggcacccgc atcaccggcc tggaccgcaa
    10321 cgccgactgg cgcaccgggc ccggcttcgt ggtcgaggcc gacgagtccg acggcacctt
    10381 cctgcgggtc ggggccaccg acgcgctggt caccaacgtc gaggccgacc acctgtcgta
    10441 ctgggggacc gaggacgccc tggtcgaggg cttccgcacc ctcgtcggcg ggctggacgg
    10501 caccgccgtg ctctgcctcg acgaccccgg cgccgctgcc ctggcgccgt tcgcagggcg
    10561 caccgtcacc tacggcaccg acgacgccgc cgacctgcgc atcaccgacg tcggccccgc
    10621 cggcatcggt gtgcgcttca ccctgcacca cgacggggac gcggtcgagg tcgaggtgcc
    10681 ggcggcgccc gggctccaca acgctcgcaa cgccgccggg gccctggcgg cgtgcctggt
    10741 gcggggcgtg cccctcgccg acggcgcccg tgccctcctc ggcttcgccg gtgtcgcccg
    10801 ccggttcgag cagcgcggcg tcgcggatgg ggtgaccttc gtggactcct acgaccacct
    10861 gcccaccgag gtcgccgccg ctttggccgc cgcccgcacc ggtcccttcg accggatcgt
    10921 ctgcgtcttc cagccccacc gctacacccg cacccgggac ctggcccaca ccttcgccga
    10981 cgccttcgtc gacgccgacg tcctcggggt caccgagctg tacccggccg gcgaggcgcc
    11041 catcgagggg gtcagcggcg ccctggtccg cgatgcggtc gtggccgccc accccgacgc
    11101 cgacgtggcc ttcctgcccg acctccccgc cgccgaggcg tggctgctgg acgtgctgcg
    11161 cccgggcgac ctgtgcctca cgctcaacgc cggcgacctc accgccacgc ccgaccgggt
    11221 gctggccgcg ctccgggagc gcggctcgtg agccccgatc cgctcacgat cgccggcgag
    11281 gtcctcggcg acctcgccca gccgatggtg cccctcgggc cgttcaccac ctacaaggtc
    11341 gggggcccgg cggcgctgtt cgtggaggtc accgacgacg acgtcctggc tgcggtcggc
    11401 gccgccgtcg ccgccagcgg ggtcgcggtg ctggtcgtgg gcaacggctc caacatgctc
    11461 gtctccgacg ccggcttccc cggcctggcc atccgcctcg gggacgccta cgcgacggtc
    11521 gaggtcgacg gcaccgaggt caccgccggc ggcgccgccc tgctccccgt ggtggcccgc
    11581 cagagcgtga aggccggcct gaccggtttc gagtgggccg tgggcgtccc gggatccatc
    11641 ggtggcgcgg tccggatgaa cgccggcggg cacggctcgg acatggcagc cagcctgctg
    11701 ggggtccgcg tcttcgactg ccgcacgggg gaggatgcga acgtgcccgc gtccgccctc
    11761 gagctcggct accgacgctc cgccatcacc tccgaccagg tggtgctgcg ggccacgctg
    11821 ggcctggccc tggacgaggc cggcaccggc tcggacacgc tctccgagat cgtccgctgg
    11881 cggcgggcca accagcccgg cggccagaac gccggctcgg tgttcaccaa ccccgacggc
    11941 gacagcgccg gccgcctcgt cgacgcggcc ggctgcaagg gcctgcgccg gggcaccgcc
    12001 gaggtctcgc ccaagcacgc caacttcatc caggccgacg ccggcggtct cgccgacgac
    12061 gtcttcgccg tcatggtcgc ggtgcgcgac gcggtcgagg cggacagcgg cgtgcgcctg
    12121 caccccgaga cccgcctggt cgggttcgac gaggccgagg tcacctggtg agcggccccc
    12181 gggtgctcga ccgcgacgcc gagcaggccg cgccgccgct ggctgcgggc gtcgacccgc
    12241 gcttccgggc ccggcgcatc gccgtccgca aggacgccgg ccgcaagcgc ctcaagcggc
    12301 tgctcctgct ggtgggcgtc gccgtgctcg ccctggccgc cgtcgtcgtc ctgcgcagcc
    12361 cggtgctcga cgtcgacgag gtcgtggtgg ttggcacctc ccgcctcgac ccggccacgg
    12421 tccggtccga ggccggcatc ggacagggct ccccgctgct gctcgccgac ctcggcgccg
    12481 ccgccgaccg gatcgagtcg atgccgtggg tcgccgaggc cgaggtcacc cgtgacctgc
    12541 ccggacgcgt cgacatcgcc atccgcgagc gcgagcccgt ggccgtcgtg tccggtgccg
    12601 gcaccgccgt gctcgtcgac gtcgacggtc gggtcctcga ggtcgccccg ccggtcgccg
    12661 gcgcgttcgt ccaggtcgtc gccgccgagg ccccgcccga gcccggcgac gtcgtcgatc
    12721 ccgagctgct caccgcggtc ggcctcgccg gccggctccg cgtcaacccc gccggcgccg
    12781 tggccgcggt ccacctcgag cccagcctgc agctgcacct cgccgagggc ggggtcgtcg
    12841 acctcggcga cacgtccgcc atcgacgcca aggtcgaggc gttccggacc gtccacgccc
    12901 gggtcgaccg gtcgtgcctg gccaccatcg acctcaccgt gccgacccat ccggtgctca
    12961 cccgcgacca ggggtgcggc ggtggcggtt gaccgatccc cggccaccct cgtaccctct
    13021 cgacccacac ctgacatcga gggtgagccg acctcgacct cgactcgaac ttcaccccgg
    13081 gcggtccgtg ccgcccggtg cgccgcgcct gtcggctgcg gcaccatcac ccgaccggga
    13141 ccgcgacctc gcgtaccctg atccgagcgg tcctccctac cgagaccgag cggctgaccc
    13201 tggaggaatc acgactcatg gctgcgaacc cgcagaacta cctggctgtc atcaaggtgg
    13261 tcggcatcgg cggcggtggc gtgaacgcgg tcaaccgcat gatcgacgcc ggcctgaagg
    13321 gcgtggagtt catcgcggtg aacaccgacg cccaggc
//
LOCUS       DATISU010000286        24517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_763173, whole genome shotgun sequence.
ACCESSION   DATISU010000286 DATISU010000000
VERSION     DATISU010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 24517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24517
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763173"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..339
                     /locus_tag="VLR27_11425"
     CDS             130..339
                     /locus_tag="VLR27_11425"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HSP04107.1"
                     /translation="MTKNLTVRLPDDLAADAEAVARAEGTSLNETIKDALADAVERRR
                     KDPEFKKRLRDIVKQDRELLERLAK"
     gene            336..746
                     /locus_tag="VLR27_11430"
     CDS             336..746
                     /locus_tag="VLR27_11430"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HSP04108.1"
                     /translation="MTVEYLDLADFLVIAEEITGIEVPVLMKMSRLSLADSALHAPQA
                     GYGDVDLYPDFIDKAAVLLVRLVMNHPLPDGNKRAGWVSMRMFVSINDWSWDPHPDVD
                     DAERTVMAIASGDLDEAATATWLRQHLVPPAVGE"
     gene            1141..1359
                     /locus_tag="VLR27_11435"
     CDS             1141..1359
                     /locus_tag="VLR27_11435"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HSP04109.1"
                     /translation="MKLSVSLPDDDVEYLDEYARVQRLESRSAALHKAVRLLRASELG
                     SAYEDAWTEWADSDDDSVWDAVVDHGLA"
     gene            1359..1706
                     /locus_tag="VLR27_11440"
     CDS             1359..1706
                     /locus_tag="VLR27_11440"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HSP04110.1"
                     /translation="MRRGEVRWVDLDPVRGSESAKRQPAVIVSNDGANTTASRLGRGV
                     ITVVPVASSTARVHPFQVFLDADDAGLPRDSKAQAEQVRSVSVERIGARVGRLTAPQL
                     AALDEALRLHLAL"
     gene            complement(1819..2361)
                     /locus_tag="VLR27_11445"
     CDS             complement(1819..2361)
                     /locus_tag="VLR27_11445"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HSP04111.1"
                     /translation="MPTQMVAFLRAINTGGRRLSNDALLEPFRAAGLDDVGAYQAAGN
                     VTFLADRDPESLEAGLAPLLRAAYGFEAPVFVRTADELGARISALPFTAADLDATEGR
                     TQITFLASPPSPAQIDDVLGLVPDEDRVAFVEREWFWLPRAGVSTSALPVVRIERILG
                     PMTMRTVGTVERLLAKLDRA"
     gene            2436..3614
                     /locus_tag="VLR27_11450"
     CDS             2436..3614
                     /locus_tag="VLR27_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04112.1"
                     /translation="MQSDPRVLVGTRKGLFTLSGSADRGRWTLGDPEFLGHIIHHAVV
                     DPRDGETVLVAAKTGHLGPTVFRSCDQGRTWSEATRPPAFRAGDPHGRAVRSVFWLTP
                     GPADEPGSWYAGASPQGLFRTDDGGDTWEPVDGWNDHPSWGEWAEWPDVEGTPDGSML
                     HSVNVDPRDADHLYIGLSGGGVFESTDGGSDWAPLNAGCAADFLPDPDVPYGHDPHCV
                     RVHPADPDRLYMQNHCGIYRLDRPSTRWTRIGDNMPRDVGDIGFPVELHPRDRDTVWV
                     FPMDGTDVWPRTCPDGRPAVYRTRDGGDTWEQQCVGLPERGWLTVKRQAMTTDAADPV
                     GVYFGTTSGEVWGSADEGGSWACLASHLPEIYSVEVAGTPGGCASPRRCAPTPAARRT
                     "
     gene            3563..3817
                     /locus_tag="VLR27_11455"
     CDS             3563..3817
                     /locus_tag="VLR27_11455"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSP04113.1"
                     /translation="MRLPTPLRSYTGGEANVTATGTTVAEVLADLDRQFPGLRFRVVD
                     EQDRLREHMTVWLDGERCRDLSASLDGLDELVLMQALSGG"
     gene            3858..4976
                     /locus_tag="VLR27_11460"
     CDS             3858..4976
                     /locus_tag="VLR27_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSP04114.1"
                     /translation="MPRQLHSTVTDDGTIVLSIAEVDIPEPREDQVVIRVEAAPINPS
                     DLGPMFGPADLRTVQQSGDGSERTITAEIPERFRSMVQARVGVPVPIGNEGAGTVVAA
                     GSSDAAQALLGKTVATLGGAMYAELRLAPADQCLVLPDGVSAAEGASCFVNPLTTLGM
                     VETMRLEGHTALVHTAAASNLGQMLVKLCLSDGVPLINIVRRQEHVDLLRSLGATHVC
                     NSSDDDFRSQLTEAVAELDATIGFDATGGGTLASDILSAMETAQSRKEGAVGRYGSTR
                     HKQVYIYGGLDRSKTELDRSYGMAWAVGGWLLPYFLDRVGPERAQQLRERVASEITTT
                     FASSYTATVSLEEALSAEAVSVYGRQATGEKYLVDPTR"
     gene            complement(4979..5698)
                     /locus_tag="VLR27_11465"
     CDS             complement(4979..5698)
                     /locus_tag="VLR27_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSP04115.1"
                     /translation="MAGGSERTASGLPLPPSARRAGAASAVGVAAMLALLAVLSRVVE
                     LPSSRSITLDLAGAVGRSPDSVHAAVEVATEVGVVVLVVAVLWAAWRARGSVPALGRL
                     LAGGIGAVLAYVGSEVLKVFAAQPRPCQQVAALASDACPPAGDWSLPSNHAVIAVGLA
                     TALLLTFPRWWRWVVPVAAAVLVTRVALGVHYPHDVLDGALLAVVVVTGSVLVLDGVA
                     GRAVAAVAAHPRLGRVLRRAG"
     gene            complement(5817..6674)
                     /locus_tag="VLR27_11470"
     CDS             complement(5817..6674)
                     /locus_tag="VLR27_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04116.1"
                     /translation="MNDDEMYVHRPGIVVAREHVSRYPRHYASLGLFLVAMLLVPTVG
                     PRDLADLASSSSPAGATAPEDMLPLTPPSTAAAAPAAPPSTAARAVADAFGIDTDTAA
                     AVIDGAAGGSTPPPAPSGGGEAPAPAPAPDDPEDAGPAGVPLPSPPAVPLPAVPEELQ
                     PLLAAVGPLTNQGCSGLGLAAVVVAVAGTAAQDVPVGQVLPYLAPVYSACATFPPPAG
                     EPTVCEVDRAAHDAGYPSDVSGLMKTPNAIGTGVDVLYGIEAAIEAYTGQSAGLVAGI
                     ADQLGCSAG"
     gene            complement(6671..7807)
                     /locus_tag="VLR27_11475"
     CDS             complement(6671..7807)
                     /locus_tag="VLR27_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04117.1"
                     /translation="MTALALRSVAPGQRRPVSRWVAGGAVAATLAGGALLLVPGADDG
                     DPPPRTGSAGVVLDYEITYRTTFADGTEVEEEVAISRPFRSAVRSGETGRLSDAGVLA
                     TAGGDGRWLAIEVPIAPASGDVRIDAVLDAAVAEGHVEPGATRRIAGTTCRDHRFGGP
                     VSAGVLTPVGTVPGEHADVCIADGGLVLRERWVVDGEVRRTREAVSVAVRSVDDDRFA
                     VPSDARPLPFEQGGGSTEAVADDHDAGFAEHWTLATPEGFDHAGRWVVAPPAPQALEP
                     GEVRGPEVALVSDAWVRGVDLLVLDQGATMPGVAPPWDERPVVARHDLGPAGTAEVAW
                     DLRASEVRVLRPDGGFVRIAGTLPPDELLAIARTLTTTSPGGAS"
     gene            complement(7804..10986)
                     /locus_tag="VLR27_11480"
     CDS             complement(7804..10986)
                     /locus_tag="VLR27_11480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP04118.1"
                     /translation="MTTVDPATARTRSRRSEPLRAAGREDGPSDPVLVEDPAVAVPVD
                     PPVEVPPPIPPDLDAVFDLVPPPAPVEAGRSRWDIRRVTGTAPALPLLVLFGLNAVDE
                     LDRTAFGVLAPDIRDHFGMSNQGILSLITAFSLVVLVLGLPIAHQADRRSRLGMARGG
                     AVAWAGFSVLTGVAPAIGVLLLARIGSGVGKAVNDPTHNSLLADYYPPDTRARVYYLH
                     KMANTVGQIAGPVTAGVLATLFTWRTPFLVFAVPTVVLVVISLRLVEPSRGVWDRRAA
                     GADDATVEVEEEPLPFKEAWRTLWAIRSLRRVFYALPFLSASLIGLAALLSLFWEEVY
                     GLSPAERGVLESASEAFQLVGIAIGAVVVQRALTKDPSHVVKLLAVAGFIAGGAIAAM
                     ALSPVFLGSVVARVVFAVLAVALIPGIYAVGSLVLPARCRALGFSAAGIFALPGILFL
                     PVAGAIGDAHGLRAGMLVLIPVYLLGSVVLASSGLFVNADIETNRRSAKAAATRPEAR
                     SLGAAVMDRPFDDPTTVERPPALLRVEGVDASYGQTQVLFGVDLEVAEGEIVALLGTN
                     GAGKSTVLKAVSGLLAKDEGRVVFDGEDISGLDANATAAMGIAQVPGGRGVFPGLTVA
                     ENLRASAWMFRSDKAHVAWATERSLDLFPRLRERLHTPAGALSGGEQQMLSLSQAFVA
                     KPKLLMIDELSLGLAPTIVERLLEIVREIHREGTTIILVEQSVHTALRLADRAVFMEK
                     GEVRFEGSTAELLERTDILRAVYLKGSGAQEPADPVPATPRRPAADVLEAPPVLEVHG
                     LTKRYGGVAAVSDVSFTLHQGQVLGLIGPNGAGKTTIFDLVSGFQPLDGGRVCLFGRD
                     VTAWPAHARARAGLGRMFQDARLFGSLTVREAIAVSLERQVEVGDPLSSMLGMPGARA
                     SEEKLGLRVEELIELVGLGSFRDKFVDELSTGSRRMVEIAALLGIGPKVLLLDEPSSG
                     IAQKEAEALGPILLDVQRELDASILVIEHSMPLLTSIADHLVALDSGTYLTEGEPEAV
                     LTHPAVAESYLGGAEVDADGWANTDGSDAG"
     gene            complement(10983..13139)
                     /locus_tag="VLR27_11485"
     CDS             complement(10983..13139)
                     /locus_tag="VLR27_11485"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04119.1"
                     /translation="MGSFLDLPVPERLRPLVGAGIVLVLWGVLARLMGRGLPVGIIIR
                     GAVFGSLIALLAIGVVLVYRANRIVNFAQAELGSVAAVLAIQFKLVWGWNYFVAVVVA
                     LVLAGAMGGLIDRLVIRRFHRAPRLILTVATIGLAQILNALSIIIPLMIGGLAAGTFA
                     TPFAFRFDVHPQVFGGDHVMVMAVVPLVMLGLVWFLRFTDYGVAIRAAAENGDRANLV
                     GIPVKRLSTLVWTLAGFLSATAVILRVPLVGFASFQSVSGSGNALLLRTLAAAVLGRM
                     ESLPRTVVAAIGIGIFQEAAAWQYSDTDFVDALLVGVILIGLLLQKDFFSRAAETGIS
                     TWKAVREIRPVPAELRDLPEVRWAGFGLTTLLTVGVITLPLWASPSQEQAAALVAIYA
                     IVALSLFVLTGWAGHISLGQFAFVGFGGAACGVLYGRHGWDLMLALPAGVVFAAGAAV
                     LVGLPALRIRGLFLAVTTLAFAVTAETFLLEDRYVPWLIETRLDQPVLWGRFPLSEGW
                     QQYFLCLVGLLAAIVAVHNLRAGRMGRAIIAVRDNEANAEAAGIDATRVKLAAFAISG
                     GLAGFAGGLYVINQQGLFSDAFGAAVSIRMFSMVVIGGLGSLPGAILGAVYVRGAEFF
                     LPPEYELLASGFGILLLLMFLPEGLGGLVYRGRDAYLRRVARKHGLVVPSLLADERVE
                     DPRDQPTVDAMIADGDVALVDAAPPEPQRRPEEVRT"
     gene            complement(13141..14925)
                     /locus_tag="VLR27_11490"
     CDS             complement(13141..14925)
                     /locus_tag="VLR27_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04120.1"
                     /translation="MSSERRPVVTRIGPLGVVAATWVVIAVLFPTTEPDAMVSGGPGG
                     SSTIELADGASGTAAGGRTATSAGSGDAAGPATGAGADAGADAAGGAAGGDGAGDAPG
                     APEATAAGASGTTVGGLPCEPGVRQIPVSTYAVPCLPAWSGDNGGTTARGVTADEIVI
                     VERVFPETANSQAVGAVTEQAGAASAEQQDAIFEVFKQYLNDHYELYGRKVRWIRYES
                     RFGSSTDEAQSKGREAACLDAGVVADELGAFAVVGTRAAPVSGPFAECAAQRGVAVLN
                     GAAYFPERFYAALHPYVWAFTMECERISYQVAEYVGKRLAGKPAKWAGDPLLASQDRK
                     FATYVPNNDEYQHCVDITERVGKEQYGVPASSRYNYTLDVSRFPDEAARGVVQFKADG
                     ATTVVMACDPISAIFLTQSATSQAYYPEWFLIGTALQDTDLFAQLYDQDQADGHMFGM
                     SQLGATSKIVGPDSEPGRLYELITGQRMVEGASGGYSGLILTYNFLQAAGPGLTADAI
                     ATGARTIPPGGAPDFSLGYWSFADGPDGTAGAGDHTAVDDSREIYWTRSKVSPENGTP
                     GTFVETYGGQRFRNGEWPAEDPPIYPGS"
     gene            15152..15556
                     /locus_tag="VLR27_11495"
     CDS             15152..15556
                     /locus_tag="VLR27_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HSP04121.1"
                     /translation="MSDSQTMFDTHPRQLGVDAAVLARTVDALVACANTCTQCADACL
                     GEDDPAAQVRCIHRNLDCADICAATARVLSRRTEPTPDVARAQLQACIAACDSCAQEC
                     EQHAEHMEHCRVCAEHCRRCEQACQELLSALG"
     gene            complement(15566..16606)
                     /locus_tag="VLR27_11500"
     CDS             complement(15566..16606)
                     /locus_tag="VLR27_11500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSP04122.1"
                     /translation="MDVPGRLGEGRSLSASVEGVAPIAIIDFLANAESVVASLGEHTC
                     VEDWVLARCLGDEWVVLAGHGRHGYRPGDVLDRPAGASEHLAALASAWADDVVVDLTE
                     GAVEPRRGQPASEEPDDFDGLVAFPLWGGDGLFGAICALPADPVEEAELRRNESMVRL
                     TVELLTSVLGLDLDRSRLQRRLDAAESAALSDPMTGLGNRRAFERAVDREEARCARFG
                     HRAGIVIIDLDGLKDMNDTWGHGAGDDLIRRAGEAIRNTLRSADQAFRIGGDEFALLL
                     PEVTVDGLGGVRNRLAEAMAAAGVAASMGSAIREPAGSLHASVRAADAAMYERKRARA
                     LGPGRSRGRIRG"
     gene            16734..17024
                     /locus_tag="VLR27_11505"
     CDS             16734..17024
                     /locus_tag="VLR27_11505"
                     /inference="COORDINATES: protein motif:HMM:NF025385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HSP04123.1"
                     /translation="MAETRSERIRWTAVSKVSAVLGAIATRKLLQALWPSGDDGTGPP
                     FNPADRRVAWSTAVQWGIAAGVGAGLARVVSQRAAAAGWEAATGAPPPGLKT"
     gene            17047..17907
                     /locus_tag="VLR27_11510"
     CDS             17047..17907
                     /locus_tag="VLR27_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04124.1"
                     /translation="MLSQRWRASIPVVAAVAVVAALSLLTVTVMSNIGGGDDERSLVD
                     VDGSTSTTEATRRIAPRPSTTTEAPEVLGDTTERDPDPDADADPTTTTTVPGPPAPGV
                     ATPAPAPAPAPPAPGPAPTPGPTAPPTTSAPEPTAPPTTVCRNSADPSCGDFSWDPQP
                     EQSEVEVFAVTTPDATSAGEPVTFVVDYIDPAGVTAIGACLNWSTTDPAVVDTSSCQV
                     VATDCDRYGPHDPPARSEERIRLEQTIVFETPGEHEVTISGNIATHLADRCESPYTTS
                     FSRTFTIVVS"
     gene            complement(17900..18541)
                     /locus_tag="VLR27_11515"
     CDS             complement(17900..18541)
                     /locus_tag="VLR27_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HSP04125.1"
                     /translation="MSTQRTVRRLRPDPIPDDVLERVLQAACWAPTGGNQQPWRVVVV
                     TDDEGKAGLQAIYQPEWDRYVAGFMARLEGLPEEQLAPWRKIAAAGDHLAQHLAEAPA
                     VLVFCADPSQMAITDARLDRISIVGGGSVYPAVQNLLLACVAEGLGCAITTLHCRRED
                     EVRALLDIPDGWATAAMVPIGYPVLGGHGPITRQPPARLAHRDRFGAPWPGVS"
     gene            18684..19283
                     /locus_tag="VLR27_11520"
     CDS             18684..19283
                     /locus_tag="VLR27_11520"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HSP04126.1"
                     /translation="MDATQVADVFGAMATMAVVGVVLAGIAALVHRPTRDLVRDLVDE
                     HGRISAWLVAAIATGGSLWFSESAGFPPCELCWYQRIAIYPLVVVLGIRALRRHGSRD
                     LRLAGLVLVGLGLVVNLWHVAIETFPDLDTGACDAAVPCTVRWVEGLGFFTIPRLATV
                     TLALIGLALLLDRTDGRGQDDEHHPYDPQSSTPVGGPTP"
     gene            19280..19930
                     /locus_tag="VLR27_11525"
     CDS             19280..19930
                     /locus_tag="VLR27_11525"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSP04127.1"
                     /translation="MSTTKQRTRPGGPVTRRSYTPFIVLGVILVVALGIALLASSGGD
                     DDGGADSELLATAGITATGDRLPALPEGGDDPAEGTPAPTLEGVTPAGQGTNVAFDQP
                     TLLAFMAHWCPHCQAELPELVELAESGDLDDLDAVVVLTGTDESAPNYPPGPWVDREG
                     WTGRVLLDDDSGTAASAFGLSSYPFLVLVDGDGNVVDRNAGGLGVDGLREFVAQVG"
     gene            19978..21276
                     /locus_tag="VLR27_11530"
     CDS             19978..21276
                     /locus_tag="VLR27_11530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP04128.1"
                     /translation="MTAERRAEDQAELFRKRGAEVLHAPTVHTVDLTGDAALRATTES
                     VIADPPDWVVATTGFGMRLWFEAADAWDLGDALVGALGTGQVVARGAKAQSACRQRGL
                     EVRWRAPGESMPEVVDWLAAQAGFGEASVVVQLFDPEDHPSTAELVAMAGRVTEVPVY
                     RWRRPDDEGPMRDLTHRIARREVDAVTFTSQPAVRFLLEVAAEEGVADELVAACNDGA
                     VLPVCVGPVCAEPIAAAGIRTAIWPDPYRLVPMVKLAEERLAALGGDSSANRRGESST
                     NRSPATFEVVDPHSADARAAMAAYFDELDGRFPTGFDPGDARTGDDAALAPPSGAFVL
                     ARQDDRVVACGGIQTIGDGIGEIKRMWVADDVRGQGLGRRLLAELEARSAALGHRVVR
                     LDTNGALTEAIALYVRSGYAEIERYNDNPYAQRFFEKPLP"
     gene            complement(21281..22816)
                     /locus_tag="VLR27_11535"
     CDS             complement(21281..22816)
                     /locus_tag="VLR27_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04129.1"
                     /translation="MSDEKPTNDDAAKPKLPKGATTAAAWTTPDGGSLPYTAEATWMA
                     LRDDDDEPIADIFSVSYLVDDAGAARPVTFVFNGGPGASSAYLHVGALGPRRVGFDER
                     GMPLAPPTALVDNAESWLAFTDLVFVDPVGTGWSRIVKDDAAKGGGDKGDHSGNGGGK
                     GGGGEGRDPKAFFGVNKDLDSLTELMARWLSGHDRWASPVFVAGESYGGFRAAKLARL
                     ANERGIGLNGTILISPALEFALLDPSDYDVLPWVDLVPTMALAAHHHGRSRAFPPDAT
                     PGAVRQMAEDFATGPYATALIRGASMPAAERAEVVTRMADLLGLPEEVVDQHECRISM
                     TRFARELLRDEGTVIGLYDATATGRDPFPDRDGMAWPDPTLSGIERVFNAGINARLRT
                     ELGVRTDREYHLLSMAVNKDWKIDFDQHALESQVGATDDLRYGMALNPHMKVFLTHGL
                     YDMVTPYFASERIRNLLRLDEVTASSLTVAHFSGGHMFYAWEDSRTAFRDAIAGFYEG
                     ALR"
     gene            complement(22872..23435)
                     /locus_tag="VLR27_11540"
     CDS             complement(22872..23435)
                     /locus_tag="VLR27_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP04130.1"
                     /translation="MTDVPEAGSAGTASPNAMRAAETDHGDAVDRTSEWQRLRERHLE
                     AGPGRRMSSARGVHHVALISGDLERTIEFYSGLLEFPLVELFENRDYRGSTHFFFDIG
                     AGNALAFFDFPGLDLGRYAEVLGGLHHLAISVDPLRWERLKGKLDEAGVEYHHESGSS
                     LYFRGPDGERIELISDPLGEMYGEVIG"
     gene            complement(23432..24178)
                     /locus_tag="VLR27_11545"
     CDS             complement(23432..24178)
                     /locus_tag="VLR27_11545"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSP04131.1"
                     /translation="MNIPTTELRRADERFHTRISWLDSRHSFSFGGHYDPSEVGHGLL
                     IVSNDDRVAAGGGFSTHPHRDMEIVTWVLDGELEHRDSEGNEGVIYPGLAQRMSAGRG
                     IMHSEMNHSTTADVHFVQMWVPPHEPGITAGYEQLDLNAELDAGGLVPVASGRGHDSA
                     ISIHQQHAAMWVARLEAGRSVDLPAAPFVHLFVARGAVELSQTDGAPGEPLALAAGDA
                     ARLRDAGHPVVTATERSEVIVWESDLEVRR"
BASE COUNT         3512 a         8905 c         8950 g         3150 t
ORIGIN      
        1 gtgccgatcc tcggcggtgt cggctcggcc cgcttgaacg acgcgggggc gccgatcaac
       61 cggttctcct gtggaagccg agctgaacac gcggcaaagg cgcgaatgcc accgtgatac
      121 cgtggtgtca tgacgaagaa cctgaccgtg cgcctgcctg acgacctcgc cgccgatgcc
      181 gaagcggttg ctcgcgccga gggcaccagc ctcaacgaga cgatcaagga cgccctcgcc
      241 gacgcggtcg agcggcgacg caaggaccct gagttcaaga agcgcttgcg ggacatcgtc
      301 aagcaggatc gggagcttct cgaacgactg gcgaagtgac cgtcgagtac ctggatctcg
      361 ccgacttcct cgtcatcgcc gaagagatca ccggcatcga agtgccggtc ctcatgaaga
      421 tgtcgcgtct ctcgctggcc gactccgctc tgcacgctcc gcaggccggg tacggcgacg
      481 tcgacctgta tccggacttc atcgacaagg cagcagtcct tctggttcgt ctcgtgatga
      541 accatccgct cccggatggc aacaagcgtg cgggctgggt gtcgatgcgc atgttcgtgt
      601 ccatcaatga ctggtcctgg gatccgcacc cggacgtcga cgatgccgag cgcacggtga
      661 tggcgatcgc atccggagat ctcgacgagg cagcaaccgc gacctggctg cggcagcatc
      721 tcgtcccgcc cgccgtcggc gagtgacgac cgaccgccag cgaatcccaa gtacgagccg
      781 acgaggcgct gcacccctcg gggtagctgg tggtcggcca ggctgtctgc atggacggcg
      841 cctggacagt cgaggttgtg ccatcggggg ctgacctcat catcatcgaa agacttggac
      901 aagtggagac ggggacgacg acgggactcc tgccctccgc agacaaggtc ttcggagtcg
      961 gctccctcgg tgaacacggt ctggtgttcg cgctcgccga cgcggccgaa gcggtcgagg
     1021 ttcgcctcgc cagcggcgat gtcgagcggc cgcctctgac tcaggttccg gactcgccca
     1081 cgaagtactt cgccatgcca ctgcggtccg gacagcagcc gctgcgcgtt gtcgccttct
     1141 atgaagctga gtgtgagtct cccggacgac gatgtcgagt acctggacga gtacgcgcgt
     1201 gtgcagcggc tcgagtctcg atcggccgca ctgcacaagg ccgtccggct tctccgagcg
     1261 tcagagctcg ggagcgcata cgaggatgcc tggactgagt gggctgacag cgacgacgac
     1321 tcggtgtggg acgcagtcgt cgatcacggt ctggcgtgat gcggcgaggt gaggttcgat
     1381 gggtcgacct cgatcctgtc cgtggttccg agtcggccaa gcgacagcct gccgtgatcg
     1441 tcagcaacga cggggcgaac acgacggcaa gtcggctcgg tcgcggcgtg atcaccgtgg
     1501 tgccggtggc ctcgagcacg gcgcgcgtgc atccgttcca ggtcttcctc gatgccgatg
     1561 acgccggact gcctcgcgac tccaaggccc aagccgagca ggtgcggtcg gtctccgtcg
     1621 agcggatcgg agcacgggtc gggcggttga ccgcaccgca actcgccgcg ctcgacgaag
     1681 cgctgcgact tcatctggcg ctctagcaga agaagcgcag gtagaggcca gctcttcggc
     1741 gggtcgccag acacttccaa atcggtagcc gcacagtcgc tcgagccgtt ctccacccat
     1801 tcagcaaccc gcccgagctc acgctcggtc gagcttggcg agcaggcgct ccaccgtgcc
     1861 gacggtgcgc atcgtcatcg gcccgaggat gcgctcgatc cgcacgaccg ggagcgcgga
     1921 ggtgctcacc ccggctcggg gcagccagaa ccactcccgc tcgacgaacg cgacccggtc
     1981 ctcgtccggc acgagtccga ggacgtcgtc gatctgggca gggctggggg gcgacgccag
     2041 gaacgtgatc tgcgtccgcc cctcggtggc atcgaggtcg gcggcggtga acggcagcgc
     2101 cgagatccgc gcaccgagct cgtcggcggt gcgcacgaag acgggcgcct cgaacccgta
     2161 ggcggcgcgg agcagcggag cgagcccggc ttcgagcgac tccgggtcgc ggtcggcgag
     2221 gaaggtcacg ttgcccgcgg cctggtaggc gccgacatcg tcgagcccgg cggcgcggaa
     2281 tggctcgagc aatgcgtcgt tcgagagccg gcgaccgccg gtgttgatcg cccggaggaa
     2341 cgcgaccatc tgcgtcggca tctgaccagt gtcgcccagg agccgcaccg atgatccacg
     2401 agcgctggat ccgtcggagg tgcatgatga cgacgatgca gtccgatccg cgcgtgctcg
     2461 tggggacgag gaagggcctg ttcacgctgt cggggtcggc cgaccgcggg cgctggaccc
     2521 tgggtgaccc ggagttcctc ggccacatca tccaccacgc cgtggtcgac ccgcgcgacg
     2581 gcgagacggt gctggtggcg gccaagaccg gccacctcgg tcccaccgtg ttccgctcct
     2641 gcgaccaggg tcgcacatgg tccgaggcca cccggccgcc ggcgttccgc gccggtgacc
     2701 cccacggccg tgccgtccgc tccgtgttct ggctcacccc gggaccggcc gacgagcccg
     2761 ggtcctggta cgccggcgcg tcgccccagg gcctgttccg caccgacgac gggggcgaca
     2821 cctgggaacc cgtcgacggg tggaacgacc accccagctg gggcgagtgg gccgagtggc
     2881 ccgatgtcga gggcacgccc gacggctcca tgctccactc cgtgaacgtc gacccgcgcg
     2941 acgcggacca cctctacatc ggcctgtccg gtggcggcgt gttcgagtcg accgacggcg
     3001 ggtcggactg ggcgccgctg aacgccggct gcgccgccga cttcctgccc gaccccgacg
     3061 tcccctacgg ccacgacccg cactgcgtgc gcgtgcaccc cgccgacccc gaccggctgt
     3121 acatgcagaa ccactgcggc atctaccgcc tcgaccgtcc gagcacccgg tggacccgca
     3181 tcggcgacaa catgccccgc gacgtcggcg acatcggctt cccggtcgag ctgcaccccc
     3241 gggaccgcga cacggtctgg gtgttcccga tggacggcac cgacgtgtgg ccccgcacct
     3301 gcccggacgg ccggcccgcc gtgtaccgca cccgcgacgg cggcgacacc tgggagcagc
     3361 agtgcgtcgg cctgccggag cgggggtggc tcacggtcaa gcgccaggcg atgaccaccg
     3421 acgccgccga cccggtgggc gtctacttcg gcacgaccag cggcgaggtc tggggcagcg
     3481 ctgacgaggg cgggtcgtgg gcgtgcctcg cctcgcacct gcccgagatc tactccgtcg
     3541 aggtcgcggg caccccagga gggtgcgcct ccccacgccg ctgcgctcct acaccggcgg
     3601 cgaggcgaac gtgacggcga cgggcaccac ggtggccgag gtgctggccg acctcgaccg
     3661 gcagttcccc gggctccgct tccgggtggt ggacgagcag gaccggctcc gcgagcacat
     3721 gaccgtgtgg ctggacggcg agcgctgccg cgacctgtcg gcatcgctgg acgggctcga
     3781 cgagctcgtc ctcatgcagg ccctctccgg cgggtgacac agtggcctcc cgctcctccc
     3841 acggaaggcc acaccacatg ccacgccagc tccactcgac ggtcaccgac gacggcacga
     3901 tcgtgctgtc gatcgcggag gtcgacatcc ccgagccgcg cgaggaccag gtcgtcatcc
     3961 gggtcgaagc cgcgccgatc aacccgtcgg acctcggacc catgttcggc cccgccgacc
     4021 tgcgcaccgt ccagcagtcc ggcgacggct ccgagcgcac gatcaccgcc gagatccccg
     4081 agcgcttccg ctcgatggtg caggcccgcg tcggcgtgcc ggtgccgatc ggcaacgagg
     4141 gcgccggcac cgtcgtcgcc gccggctcgt ccgacgccgc gcaggcgctg ctcggcaaga
     4201 ccgttgccac cctgggcggt gcgatgtacg cagagctgcg cctcgccccc gccgaccagt
     4261 gcctggtgct gcccgacggc gtgtccgccg ccgagggggc gtcctgcttc gtgaacccgc
     4321 tcaccacgct cggcatggtc gagaccatgc gcctcgaggg ccacaccgcc ctggtgcaca
     4381 ccgccgctgc ctccaacctc ggccagatgc tggtgaagct ctgcctgtcc gacggcgtcc
     4441 cactgatcaa catcgtgcgc cgccaggagc acgtggacct gctccggtcc ctcggcgcca
     4501 cgcacgtctg caactccagc gacgacgact tccggtccca gctcaccgag gcggtggccg
     4561 agctcgacgc caccatcggc ttcgacgcca ccggtggcgg caccctcgcc agcgacatcc
     4621 tctccgcgat ggagacggcc cagagccgca aggagggggc agtcggtcgc tacggctcga
     4681 cccgccacaa gcaggtgtac atctacggcg gcctcgaccg ttccaagacc gagctcgacc
     4741 gctcctacgg catggcgtgg gcggtggggg gctggctgct gccgtacttc ctcgaccggg
     4801 tcgggcccga gcgcgcccag cagctccggg agcgggtggc ctccgagatc accaccacgt
     4861 tcgccagcag ctacacggcc acggtgtcgc tcgaggaggc cctcagcgcc gaggccgtgt
     4921 cggtctacgg gcgccaggcc accggcgaga agtacctcgt cgaccccacc cgctgacgtc
     4981 atccggcgcg tcgcagcacc cggcccagcc gcgggtgcgc ggcgacggcg gcgacggctc
     5041 gcccggccac gccgtccagg acgagcaccg aaccggtcac cacgacgacg gcgagcagcg
     5101 cgccgtcgag cacgtcgtgg gggtagtgca cgcccagggc caccctggtc accaggacgg
     5161 cggccgcgac cggcaccacc cagcgccacc aacgcgggaa cgtgagcagc aacgcggtcg
     5221 ccagtccgac ggcgatcacg gcgtggttgc tgggcagcga ccagtccccc gccggcgggc
     5281 acgcgtcgct cgccagtgca gccacctgct ggcagggacg gggctgtgct gcgaacacct
     5341 tgagcacctc gctgccgaca tacgcgagga cggcaccgat gccgccggcg agcagccggc
     5401 ccagcgcggg taccgacccc cgagcacgcc aggcggccca caggacggcg acgaccagca
     5461 cgacgacacc gacctcggtg gcgacctcga ccgcagcgtg cacagagtcc gggctgcgac
     5521 cgaccgcacc ggcgagatcc agggtgatgc tccggctgct cggcagctcg accacccggg
     5581 acaggacggc gaggagggcg agcatggcgg cgaccccgac ggccgaggcg gctccggcgc
     5641 gccgcgccga gggcggcagg gggagcccgc ttgcggtgcg ctcagagcct cctgccatgt
     5701 ctcggacggt acgcagggtg gctgggtccg tcgtccgccg tgggggtgat cgcgacggtc
     5761 gggggtccga ccggcggcgg accctggccg ctccgtgcgg cggacgctcg tcgctgtcag
     5821 ccggcgctgc agccgagctg gtcggcgatg ccggcgacca gccccgccga ctgcccggtg
     5881 taggcctcga tggcggcctc gatgccgtac agcacgtcga cgccggtgcc gatggcgttc
     5941 ggggtcttca tcaggccgct gacgtccgag gggtacccgg cgtcgtgggc ggcccggtcg
     6001 acctcgcaga cggtcggctc gccggcgggc ggggggaacg tggcacaggc cgagtacacc
     6061 ggcgccaggt acgggaggac ctggccgacc ggtacgtcct gggcggcggt gccggccacc
     6121 gccaccacga cggcggcgag cccgaggccc gagcacccct ggttcgtgag cggcccgacc
     6181 gcggccagca gcggctgcag ctcctcgggg acggcgggga gggggacggc cggaggcgac
     6241 ggcaacggga cgccggcggg tccggcgtcc tcggggtcgt ccggggccgg cgcgggggcg
     6301 ggggcctcac cgccgccgga cggcgccgga ggtggcgtcg agccaccggc cgcgccgtcg
     6361 atgaccgccg cagccgtgtc ggtgtcgatg ccgaaggcgt cggcgacggc ccgggcggcg
     6421 gtcgacgggg gggcggcggg ggcggccgct gccgtcgacg gcggcgtcaa cggcagcatg
     6481 tcctcgggcg cggtggcgcc ggcaggggac gagctcgacg ccaggtcggc gaggtcgcgg
     6541 gggccgacgg tcggcacgag cagcatggcc acgaggaaga gcccgagcga ggcgtagtgc
     6601 cgcgggtagc gggagacgtg ctcccgggcg acaacgatcc cgggccggtg gacgtacatc
     6661 tcgtcgtcgt tcacgatgcg cctcctggcg acgttgtggt gagcgtcctg gcgatggcga
     6721 ggagctcgtc gggcgggagc gtgccggcga tccggacgaa cccgccgtcc ggacgcagca
     6781 cgcggacctc actggcccgg aggtcccagg ccacctcggc cgtgccggcg gggccgaggt
     6841 cgtggcgggc cacgacgggt cgttcgtccc agggaggggc gacgccgggc atggtcgccc
     6901 cctggtcgag caccagcagg tcgacgcccc gcacccaggc gtcggagacg agcgcgacct
     6961 cgggaccgcg cacctcgccc ggctcgaggg cctgcggcgc gggcggcgcg accacccagc
     7021 gcccggcgtg gtcgaacccc tccggcgtcg ccagcgtcca gtgctcggcg aagccggcgt
     7081 cgtggtcgtc ggccaccgcc tccgtcgagc cgccgccctg ctcgaagggg agggggcggg
     7141 cgtcggacgg gaccgcgaac cgatcgtcgt cgaccgagcg gaccgccacc gagacggctt
     7201 cgcgggtgcg ccgcacctcg ccgtcgacga cccagcgctc ccgcaggacc agcccgccgt
     7261 ccgcgatgca cacgtcggcg tgctcgccgg gcacggtgcc gaccggcgtc agcacgccgg
     7321 ccgagacggg cccgccgaag cggtggtccc ggcacgtcgt cccggcgatg cgccgggtcg
     7381 cgcccggttc gacgtgaccc tccgcgacgg ccgcgtcgag tacggcgtcg atgcggacgt
     7441 cgccgctggc cggggcgatg ggcacctcga tggcgagcca ccgaccgtcc ccgccggcgg
     7501 tggcgagcac gccggcgtcg ctcaaccggc ccgtctcccc cgagcggacg gcagagcgga
     7561 acgggcggct gatggccacc tcctcctcca cctcggtgcc gtcggcgaag gtcgtccggt
     7621 aggtgatctc gtagtcgagc acgacacccg cactgccagt ccggggtggg gggtccccgt
     7681 cgtcggcgcc gggcaccagc agcagcgccc cgcccgccag cgtcgcagcc acggccccgc
     7741 cggcgaccca ccgggacacc ggccggcgct ggcccggggc gacgctgcgg agcgcgaggg
     7801 cggtcacccg gcgtcgctcc cgtccgtgtt ggcccagccg tcggcgtcga cctcggcacc
     7861 accgaggtag gactcggcca cggccgggtg ggtgagcacc gcctccggct cgccctcggt
     7921 gaggtaggtg ccgctgtcga gggccacgag gtggtcggcg atcgacgtca gcagcggcat
     7981 ggagtgctcg atgacgagga tcgacgcgtc gagctcgcgc tggacgtcga gcaggatcgg
     8041 accgagcgcc tcggcctcct tctgggcgat gcccgacgac ggctcgtcca gcagcagcac
     8101 cttggggccg atgccgagca gggcggcgat ctccaccatg cgccgcgacc cggtggacag
     8161 ctcgtcgacg aacttgtcgc ggaacgaccc cagaccgacc agctcgatga gctcctcgac
     8221 ccgcaggccc agcttctcct cgctggcccg ggcgccgggc atgcccagca tcgacgacag
     8281 cgggtccccg acctcgacct ggcgctcgag cgacacggcg atcgcctcgc ggacggtcag
     8341 cgagccgaac agccgagcgt cctggaacat acggcccagg ccggcacggg cccgggcatg
     8401 ggccggccag gccgtgacgt cacggccgaa gaggcagacc cggccaccgt ccaggggctg
     8461 gaagccggac accaggtcga agatggtggt cttccccgcg ccgttggggc cgatgaggcc
     8521 gagcacctgg ccctggtgga gcgtgaagga cacatcgctc accgcggcca cgccgccgta
     8581 gcgcttggtg aggccgtgga cctcgagcac cggcggcgcc tcgaggacgt cggcggccgg
     8641 gcgacgtggg gtcgccggca ccgggtccgc cggctcctgg gcgcccgagc ccttcaggta
     8701 cacggcccgg aggatgtcgg tgcgctcgag cagctcggcg gtggagccct cgaagcggac
     8761 ctcgcccttc tccatgaaca cggcccggtc ggcgaggcgc agcgccgtgt gcaccgactg
     8821 ctccaccagg atgatcgtgg tgccctcgcg atggatctcc cggacgatct cgaggaggcg
     8881 ctcgacgatg gtcggggcga gcccgaggga cagctcgtcg atcatgagca gcttgggctt
     8941 ggcgacgaag gcctgggaca gcgacagcat ctgctgctcg ccgccggaga gcgcacccgc
     9001 cggcgtgtgc aggcgctcgc gcaggcgcgg gaacaggtcg agggagcgct cggtggccca
     9061 ggccacgtgg gccttgtcgc tgcggaacat ccacgccgag gcccgcaggt tctcggccac
     9121 ggtcaggccc gggaacacgc cacgtccgcc cggcacctgg gcgatgccca tggcggcggt
     9181 ggcgttggcg tcgaggccgc tgatgtcctc gccgtcgaag acgacccgcc cctcgtcctt
     9241 ggccagcagg cccgacaccg ccttgagcac ggtggacttg ccggcgccgt tcgtcccgag
     9301 gagggccacg atctcgcctt cggccacttc caggtcgacg ccgaacagga cctgggtctg
     9361 gccgtaggac gcgtcgaccc cctcgacccg cagcagggcc ggcggacgct cgaccgtcgt
     9421 cgggtcgtcg aaggggcgat ccatcacggc cgccccgagc gagcgggcct cggggcgggt
     9481 ggccgccgcc ttcgccgagc ggcggttggt ctcgatgtcg gcgttcacga acagcccgga
     9541 cgaggccagg accaccgagc cgagcaggta cacggggatg agcacgagca tcccggcgcg
     9601 caggccgtgg gcgtcgccga tggcaccggc gacggggagg aagaggatgc cgggcagggc
     9661 gaagatgccg gccgccgaga acccgagggc ccggcagcgg gccgggagca ccaacgaccc
     9721 gacggcgtag atgccgggga tgagggcgac ggcgaggacg gcgaagacaa cccgcgccac
     9781 caccgagccg aggaacaccg gcgacagggc catcgccgcg atggcgccac cggcgatgaa
     9841 gcccgcgacg gcgagcagct tcaccacgtg ggacgggtcc ttggtcagcg cccgctgcac
     9901 cacgaccgcg ccgatcgcga tgccgacgag ctggaacgcc tcggaggcgg actcgagcac
     9961 gccgcgctcg gcgggcgaga ggccgtacac ctcctcccag aacagggaga gcagggcggc
    10021 gaggccgatg aggctggcgg agaggaaggg cagggcgtag aagacgcgac ggaggctgcg
    10081 gatcgcccag agggtgcgcc acgcctcctt gaacgggagg ggctcctcct cgacctcgac
    10141 cgtggcgtcg tcggcgcccg cggcccggcg gtcccacacg ccccgcgacg gctcgaccag
    10201 ccgcagcgag atcaccacga gcacgacggt gggcacggcg aacacgagga acggcgtgcg
    10261 ccacgtgaac agggtggcca gcacgccggc ggtgaccggt ccggcgatct ggccgaccgt
    10321 gttggccatc ttgtgcaggt agtacacccg cgcccgggtg tccggcgggt agtagtcggc
    10381 cagcaggctg ttgtgggtcg ggtcgttcac cgccttgccg accccggagc cgatgcgggc
    10441 gagcagcagc acgccgatgg ccggcgccac gcccgtgagc acggagaacc cggcccaggc
    10501 gacggcgccg ccgcgggcca tgccgaggcg gctgcgccgg tcggcctggt gggcgatggg
    10561 caggccgagc accagcacca ccagcgagaa ggcggtgatc agcgagagga tgccctggtt
    10621 ggacatgccg aagtggtcgc ggatgtcggg ggccaggacg ccgaacgcgg ttcggtcgag
    10681 ctcgtcgacg gcgttcaacc cgaagagcac gagcagcggc agcgccggcg cggtgccggt
    10741 gacccggcgg atgtcccacc ggctgcggcc ggcctcgacc ggcgccggcg ggggcaccag
    10801 gtcgaacacg gcatcgaggt cgggcgggat ggggggcggc acctcgacgg gcgggtccac
    10861 cggcacggcc acggcggggt cctccaccag caccggatcg gagggcccgt cctcccgacc
    10921 ggcggcgcgg agcggctcgc tccggcggga ccgggtgcgg gcggtggccg ggtcgacggt
    10981 ggtcacgtcc ggacctcctc gggtcggcgt tgcggttccg gcggggccgc gtcgaccagc
    11041 gcgacgtcgc cgtcggcgat catcgcgtcg acggtcggct ggtcccgggg gtcctcgacc
    11101 cgctcgtcgg cgagcaggct cggcacgacc agcccgtgct tgcgggcgac ccggcgcagg
    11161 taggcgtcgc gcccgcggta cacgagccca ccgaggccct cgggcaggaa catgagcagc
    11221 agcaggatgc cgaagccgct ggcgagcagc tcgtactcgg gtggcaggaa gaactcggcg
    11281 ccgcgcacgt agacggcgcc gaggatcgcg cccggcagcg agcccaggcc gccgatgacg
    11341 accatggaga acatgcggat ggacaccgct gcgccgaacg cgtcgctgaa caggccctgc
    11401 tggttgatga cgtagaggcc gccggcgaag ccggccaggc cgccggagat ggcgaacgcg
    11461 gcgagcttca cgcgggtggc gtcgatgccg gcggcctcgg cgttggcttc gttgtcccgc
    11521 acggcgatga tcgcccggcc catccgaccg gctcgcaggt tgtggacggc gacgatggcg
    11581 gccagcagtc cgaccaggca caggaagtac tgctgccagc cctccgacag cgggaaccgg
    11641 ccccacagca ccggctggtc gaggcgggtc tcgatcagcc acggcacgta gcggtcctcg
    11701 aggaggaacg tctcggccgt gaccgcgaac gccagcgtgg tgacggcgag gaacagcccc
    11761 cggatccgca gcgccggcag gccgaccagc accgcggccc ccgccgcgaa caccaccccg
    11821 gccgggaggg cgagcatcag gtcccagccg tggcgcccgt acagcacacc gcacgccgcg
    11881 ccgccgaagc cgacgaaggc gaactggccc aggctgatgt ggccggccca cccggtgagc
    11941 acgaagagcg acagggccac gatggcgtag atcgccacca gggcggcggc ctgctcctgg
    12001 ctcgggctcg cccacagggg cagggtgatg accccgacgg tgagcagggt ggtgagcccg
    12061 aagccggccc accggacctc gggcaggtcg cgcagctcgg ccggcaccgg ccggatctcc
    12121 cggaccgcct tccacgtcga gatgccggtc tcggccgccc gggagaagaa gtccttctgc
    12181 aggagcagcc cgatgaggat gacgccgacg agcagggcat cgacgaagtc ggtgtcggag
    12241 tactgccagg cggcggcctc ctggaagatc ccgatgccga tggcggccac gaccgtgcgg
    12301 ggcaggctct ccatgcgccc gagcaccgcg gcggccaggg tgcgcagcag cagggcgttg
    12361 ccggaaccgg acacggactg gaacgatgcg aagcccacga gcggcacccg caggatcacc
    12421 gccgtcgccg acaggaaccc ggcgagggtc cacaccaggg tcgacaggcg cttcaccggg
    12481 atgccgacga ggttcgcccg gtcgccgttc tcggccgcgg cccggatggc gacgccgtag
    12541 tcggtgaagc gcaggaacca gaccaggccg agcatcacca ggggcacgac cgccatgacc
    12601 atgacgtggt cgccgccgaa cacctgcggg tgcacgtcga agcggaaggc gaagggcgtg
    12661 gcgaaggtgc cggcggcgag gccgccgatc atcagcggga tgatgatcga cagcgcgttg
    12721 aggatctggg ccaggccgat ggtggccacg gtgaggatca ggcgtggcgc ccggtggaac
    12781 cgccggatca ccagccggtc gatgagccca cccatcgccc cggcgagcac cagcgcgacg
    12841 accacggcga cgaagtagtt ccacccccag accagcttga actggatggc gagcacggcg
    12901 gcgaccgagc ccagctcggc ctgggcgaag ttgacgatgc ggttggcgcg gtagacgagc
    12961 accacgccga tggcgagcag ggcgatgagc gagccgaaca cggcgccgcg gatgatgatg
    13021 ccgacgggca ggccccgacc catcaaccgg gccagcacgc cccacaggac gaggacgatg
    13081 ccggcgccca ccagcgggcg cagccgttcc gggacgggca ggtcgaggaa ggagcccacg
    13141 tcaggacccc gggtagatcg gcgggtcctc ggctggccac tcgccgttgc ggaaccgctg
    13201 gccgccgtag gtctcgacga acgtgccggg cgtcccgttc tcgggcgaca ccttcgaccg
    13261 ggtccagtag atctcgcggg agtcgtcgac cgccgtgtgg tcaccggcac cggccgtgcc
    13321 gtcggggccg tcggcgaagg accagtaccc gagcgagaag tccggggcgc cgcccggcgg
    13381 gatggtgcgg gccccggtgg cgatggcgtc ggcggtgagc cccggccccg ccgcctggag
    13441 gaagttgtag gtgaggatca gcccgctgta gccaccggag gccccctcga ccatgcgctg
    13501 gccggtgatc agctcgtaga gccggcccgg ctcgctgtcg ggcccgacga tcttggaggt
    13561 ggcgccgagc tggctcatgc cgaacatgtg gccgtcggcc tggtcctggt cgtagagctg
    13621 ggcgaacagg tcggtgtcct gcagcgccgt gccgatgagg aaccactccg ggtagtacgc
    13681 ctgggaggtc gcgctctggg tgaggaagat cgccgagatc gggtcgcagg ccatcaccac
    13741 cgtcgtggcg ccgtcggcct tgaactgcac gacgccccgg gcggcctcgt ccgggaaccg
    13801 gctgacgtcg agcgtgtagt tgtagcggga gctcgccggc acgccgtact gctccttgcc
    13861 gacccgctcg gtgatgtcga cgcagtgctg gtactcgtcg ttgttcggca cgtaggtggc
    13921 gaacttgcgg tcctgggatg cgagcagcgg gtcccccgcc cacttcgccg gcttgcccgc
    13981 caggcgcttc cccacgtact cggccacctg gtacgagatg cgctcgcact ccatggtgaa
    14041 ggcccagacg taggggtgga gcgcggcgta gaagcgctcc gggaagtagg ccgccccgtt
    14101 gagcaccgcc accccccgct gcgccgcgca ctcggcgaag ggcccgctga ccggcgcagc
    14161 gcgtgtcccc acgacggcga aggcaccgag ctcgtcggcc accacgccgg cgtcgaggca
    14221 ggcggcctcc cggcccttgc tctgcgcctc gtcggtggag ctgccgaagc ggctctcgta
    14281 gcggatccag cggaccttgc gcccgtacag ctcgtagtgg tcgttcaggt actgcttgaa
    14341 cacctcgaag atggcgtcct gctgctcagc ggaggcggcg ccggcctgct cggtcacggc
    14401 gcccaccgcc tggctgttgg cggtctcggg gaagacccgc tcgacgatca cgatctcgtc
    14461 cgcggtcacg ccccgggccg tggtgccgcc gttgtcgccc gaccacgccg gcaggcaggg
    14521 caccgcgtag gtcgacaccg ggatctggcg gaccccgggc tcgcacggca acccgccgac
    14581 ggtggtgccc gaggccccgg ctgcggtcgc ctccggcgcc cccggcgcgt cgccggcacc
    14641 atcaccaccc gcggctcccc cggcggcgtc agccccggcg tctgcaccgg cgccggtcgc
    14701 cggacccgcc gcgtcgccgg accccgccga cgtcgcggtg cggccaccgg ctgcggtgcc
    14761 gctcgcaccg tcggccagct cgatcgtgct gctcccgccc ggaccgcccg acaccatcgc
    14821 gtccggctcc gtggtcggga acagcacggc gatcaccacc caggtggccg ccaccacccc
    14881 cagcggtccg atcctcgtga ccaccggccg ccgttcgctc gacatgtctt cccccaagcc
    14941 cacttcgccg gaccggtggc ggtccgacca tctctgctga gcgcaccaac ccccggtgca
    15001 cccgattcct ggtgccgggg ttcgccgctg ggcgcgggga atcctgcctg acccgccggt
    15061 caattaccgg cggtacgccc gcccccgtct gccgatcggc gcggtcggca agggacgggg
    15121 ccactgcccg acgaaccgag gagccccctc catgtccgat tcccagacca tgttcgacac
    15181 ccacccgcgc cagctcggcg tcgacgccgc cgtgctcgct cgcacggtcg acgccctcgt
    15241 cgcgtgcgcg aacacctgca cgcagtgcgc cgatgcgtgc ctcggcgagg acgaccccgc
    15301 cgcccaggtg cgctgcatcc accggaacct ggactgcgcc gacatctgcg ccgccaccgc
    15361 ccgggtgctg tcgcgccgca cggagccgac accggacgtg gcgcgggccc agctccaggc
    15421 ctgcatcgcc gcctgcgaca gctgcgcgca ggagtgcgag cagcacgccg agcacatgga
    15481 gcactgccgc gtctgcgccg agcactgccg gcgctgcgag caggcctgcc aggagctgct
    15541 cagcgcgctg ggctgagggt cgggttcagc cccggatgcg gccgcggctg cgtccggggc
    15601 ccagggcccg ggcccgcttg cgctcgtaca tcgcggcgtc ggcggcgcgg acgctggcgt
    15661 gcaggctgcc ggccggctca cggatcgcgc tgcccatcga tgccgcgacc ccggcggcgg
    15721 ccatggcctc ggccaggcgg ttgcgcacgc caccgagtcc gtcgacggtg acctcgggga
    15781 ggagcagggc gaactcgtcg ccgccgatgc ggaaggcctg gtcggcgctg cggagggtgt
    15841 tgcggatcgc ctcgccggct cggcggatga ggtcgtcgcc ggcgccgtgg ccccaggtgt
    15901 cgttcatgtc cttcaggccg tcgaggtcga tgatcacgat gccggcccgg tggccgaacc
    15961 gggcgcagcg ggcctcctcc cggtcgacgg cccgctcgaa cgcgcgccgg ttgccgaggc
    16021 cggtcatggg gtcgctgagc gctgcgctct ccgcggcgtc gaggcgccgc tgcaggcggc
    16081 tgcgatcgag gtcgaggccg aggaccgacg tgagcagctc cacggtgagg cggaccatcg
    16141 actcgttccg ccggagctcg gcttcctcga cggggtccgc cggcagggcg cagatggcgc
    16201 cgaagaggcc gtcaccgccc cagaggggga acgcgaccag cccgtcgaag tcgtccggct
    16261 cctcggaggc cggctggccg cgacggggtt cgacggcacc ctcggtgagg tcgacgacca
    16321 cgtcgtcggc ccacgccgac gccagcgccg cgaggtgctc gctggccccg gccgggcggt
    16381 cgaggacgtc gccgggccgg tacccgtgcc ggccgtggcc ggcgagcacg acccattcgt
    16441 caccgaggca gcgggcgagg acccagtcct cgacgcaggt gtgctcgccg aggctcgcca
    16501 cgaccgactc ggcgttggcg aggaagtcga tgatcgcgat cggcgcgaca ccttcgaccg
    16561 atgcgctgag tgatcgcccc tcgccgagtc gtcctggcac gtccatccat ccctgatacc
    16621 cgaagtgccg cccaccagaa ggctacgcca ctacgcaatg tggtgcaatc ccctcagaag
    16681 gtgacaatca gacgtccggg caccggatgc cggcagtggg taagggccgg ggcatggcag
    16741 agacccgatc cgaacgcatc cgctggaccg cggtgtcgaa ggtgagcgcc gtgctcggcg
    16801 ccatcgcgac ccgcaagctg ctgcaggcgc tgtggccctc gggtgacgac gggaccggcc
    16861 cgccgttcaa cccggccgac cgccgggtgg cgtggtcgac cgcggtgcag tgggggatcg
    16921 ccgccggcgt cggcgccggg ctcgcccgcg tcgtcagcca gcgcgccgcg gccgcgggct
    16981 gggaggccgc caccggtgcc ccgccccctg gcctgaagac ctgacacggg cacgtacact
    17041 tcctgcgtgc tctcccaacg ctggcgcgcc tccatccccg tggtcgccgc ggtggccgtc
    17101 gtcgccgcgt tgtccttgct gacggtcacc gtgatgagca acatcggcgg gggcgacgac
    17161 gagcgctccc tggtcgacgt cgacggctcg acgagcacca ccgaggcgac gcgccggatc
    17221 gcaccgcgac cctcgaccac caccgaggcc cccgaggtgc tgggcgacac caccgagcgc
    17281 gaccccgacc ccgacgccga cgccgacccg acgaccacca ccaccgtccc cgggccgccg
    17341 gcccccggcg tggcgacccc cgctcccgct cccgcgccag caccgcccgc acccggtccg
    17401 gcgccgaccc ctggtcccac cgcgccgccg acgacgagcg cgccggagcc caccgccccg
    17461 cccacgacgg tgtgccgcaa cagcgccgat ccgtcgtgcg gcgacttctc ctgggatccc
    17521 cagccggagc agtccgaggt cgaggtcttc gccgtcacca cgcccgacgc cacgagcgcc
    17581 ggcgagccgg tgaccttcgt cgtcgactac atcgatccgg ctggcgtcac cgccatcggg
    17641 gcgtgcctca actggtccac caccgacccg gccgtggtgg acacctcgag ctgccaggtg
    17701 gtggccaccg actgcgaccg ctacggcccg cacgacccgc cggcgcgcag cgaggagcgc
    17761 atccgcctcg agcagaccat cgtgttcgag acccccggtg agcacgaggt gaccatcagc
    17821 ggcaacatcg ccacccacct cgccgaccgg tgcgagagcc cctacaccac cagcttctcg
    17881 cgcaccttca ccatcgtcgt cagctgacgc cgggccacgg ggcgccgaag cggtcccggt
    17941 gggcgaggcg ggcggggggc tggcgcgtga tcgggccgtg cccgcccagg accgggtacc
    18001 cgatgggcac catggccgcc gtcgcccagc cgtcggggat gtcgagcagg gcgcgcacct
    18061 cgtcctcgcg ccggcagtgc agcgtggtga tcgcgcagcc caggccctcg gcgacgcagg
    18121 cgagcagcag gttctgcacg gccgggtaca ccgagccccc gccgacgatg ctgatgcggt
    18181 cgagccgcgc atcggtgatc gccatctgcg aggggtcggc gcagaagacc agcacggccg
    18241 gggcctcggc caggtgctgg gcgaggtggt cgcccgcggc ggcgatcttg cgccacgggg
    18301 cgagctgctc ctcggggagg ccctcgaggc gggccatgaa gccggcgacg tagcggtccc
    18361 actccggctg gtagatggcc tgcagcccgg ccttgccctc gtcgtcggtg acgacgacga
    18421 cccgccacgg ctgctggttc ccaccggtgg gtgcccagca cgccgcctgc aggacgcgct
    18481 cgagcacgtc atcggggatc gggtccggtc gcaaccgtcg gacggtccgc tgcgtgctca
    18541 tcgcctggta gaggtccaac ccgctccccg ttcgctcgtg gaccgccgag tctggccgat
    18601 cattcgtgac cttggtcggt tgtcggcggt ggcgcaccgg tgccatcgtc accctgcccg
    18661 cttccccccg accggagcac gccatggacg caacccaggt cgccgacgtc ttcggcgcga
    18721 tggcgacgat ggcggtggtc ggcgtggtgc tggccggcat cgccgcgctg gtgcaccgac
    18781 cgacccgtga cctggtgcgc gacctcgtcg atgagcacgg gcggatcagc gcctggctcg
    18841 tggccgccat cgccacgggc ggcagcctgt ggttctccga gtcggctggg ttcccgccgt
    18901 gcgagctgtg ctggtaccag cgcatcgcca tctaccccct cgtggtcgtg ctggggatcc
    18961 gggcgctgcg acgccacggc agccgcgacc tgcgcctggc cgggctcgtg ctcgtcggcc
    19021 tcgggctggt cgtcaacctc tggcacgtgg cgatcgagac gttcccggac ctcgacaccg
    19081 gcgcctgcga cgccgccgtc ccctgcaccg tgcggtgggt cgagggcctc ggcttcttca
    19141 ccatcccccg cctggcgacg gtgaccctcg ccctcatcgg gctggccctg ctgctggacc
    19201 gcaccgacgg ccgcggccag gacgacgagc accaccccta cgatccccag tcgagcaccc
    19261 ccgtcggagg acccacgcca tgagcaccac caagcagcgc acgcgcccgg gcggaccggt
    19321 cacccgccgg tcgtacacgc ccttcatcgt cctcggcgtg atcctcgtcg tggccctcgg
    19381 catcgccctc ctcgcgtcga gcggtgggga cgacgacggt ggcgccgaca gcgagctgct
    19441 cgccaccgcc gggatcaccg ccaccggcga ccggctaccg gcgctgcccg agggcggcga
    19501 cgacccggcg gagggcaccc ccgctcccac cctcgaaggg gtgacgcccg ccgggcaggg
    19561 caccaacgtc gcgttcgacc agccgacgct gctcgcgttc atggcccact ggtgcccgca
    19621 ctgccaggcc gagctgcccg agctggtgga gctggccgag agcggcgacc tcgacgacct
    19681 cgacgccgtc gtggtgctca ccggcactga cgagtccgcc cccaactacc cgcccggccc
    19741 gtgggtcgac cgcgagggct ggacgggccg ggtcctgctg gacgacgact ccggcacggc
    19801 agccagcgcg ttcgggctga gctcgtaccc gttcctggtg ctggtcgacg gggacggcaa
    19861 cgtcgtcgac cgcaacgccg gcgggctcgg cgtcgacggg ctccgcgagt tcgtcgccca
    19921 ggtcggctga gcgggacgcg accgttggcg gtcgatctca ccggccgcac cgtggccgtg
    19981 acggcggagc gccgcgccga ggaccaggcc gagctgttcc gcaagcgcgg cgccgaggtg
    20041 ctgcacgccc ccaccgtcca caccgtcgac ctcacgggcg acgccgccct gcgcgccacc
    20101 accgaatcgg tcatcgccga cccgcccgac tgggtggtgg ccaccaccgg gttcggcatg
    20161 cggctgtggt tcgaggccgc cgacgcgtgg gacctcggcg acgcgctcgt cggtgcgctc
    20221 ggcaccggcc aggtcgtggc ccgcggcgcg aaggcgcagt cggcctgccg ccagcgtggc
    20281 ctcgaggtcc ggtggcgggc gccgggcgag tccatgcccg aggtcgtgga ctggctggcc
    20341 gcgcaagccg gcttcggcga ggcatccgtg gtcgtccagc tcttcgaccc ggaggaccac
    20401 ccctccaccg ccgagctggt ggccatggcc gggcgggtca ccgaggtgcc cgtgtaccgc
    20461 tggcgtcgtc ccgacgacga ggggccaatg cgcgacctga cccaccgcat cgcccggcga
    20521 gaggtcgacg ccgtcacctt caccagccag cccgcggtgc ggttcctgct cgaggtcgcc
    20581 gccgaggagg gcgtggccga cgagctcgtc gccgcctgca acgacggtgc cgtgctgccg
    20641 gtgtgcgtcg gtcccgtgtg cgccgagccg atcgcggccg ccggcatccg caccgcgatc
    20701 tggcccgacc cctaccggtt ggtgccgatg gtgaagctcg ccgaggagcg gctggcggcc
    20761 ctgggcggcg attcgtcggc gaatcgtcgc ggcgaatcgt cgacgaatcg gtccccagcg
    20821 accttcgagg tcgtcgatcc gcactcggcc gacgcccgcg ccgcgatggc cgcctacttc
    20881 gacgagctcg acggccgctt ccccaccggg ttcgaccccg gcgatgcccg caccggcgac
    20941 gacgccgccc tcgccccacc cagcggggcg ttcgtgctgg ctcgccagga cgatcgggtc
    21001 gtggcctgcg gcggcatcca gaccatcggg gacggcatcg gcgagatcaa gcgcatgtgg
    21061 gtcgccgacg acgtccgcgg ccagggcctg ggccggaggc tgctggccga gctcgaggcc
    21121 cggagcgcgg cactcgggca ccgggtggtg cgcctcgaca ccaacggggc gctcaccgag
    21181 gccatcgccc tgtacgtgcg gtccggctac gccgagatcg agcgctacaa cgacaacccg
    21241 tacgcgcagc ggttcttcga gaagccgctg ccctgacccg tcagcgcagg gcgccctcgt
    21301 agaacccggc gatggcgtcg cggaacgccg tgcggctgtc ctcccacgcg tagaacatgt
    21361 gcccgccgga gaagtgcgcc acggtcagcg aggacgcggt cacctcgtcg aggcgcagca
    21421 ggttccggat gcgctcggac gcgaagtacg gcgtgaccat gtcgtagagg ccgtgggtga
    21481 ggaacacctt catgtgcggg ttgagcgcca tgccgtagcg caggtcgtcg gtggcaccga
    21541 cctggctctc gagggcgtgc tggtcgaagt cgatcttcca gtccttgttc accgccatcg
    21601 agagcaggtg gtactcgcgg tcggtgcgca cgccgagctc ggtgcggagc cgggcgttga
    21661 tgccggcgtt gaacacccgc tcgatgccgc tcagcgtcgg gtcgggccag gccatgccgt
    21721 cgcggtcggg gaacggatcg cgcccggtgg cggtggcgtc gtagaggccg atgaccgtgc
    21781 cctcgtcccg cagcagctcc cgtgcgaagc gggtcatgga gatccggcac tcgtgctggt
    21841 cgacgacctc ctcgggcagc ccgagcaggt cggccatgcg ggtgaccacc tcggcccgct
    21901 cggcggccgg catcgaggcg ccccggatga gggcggtggc gtagggaccg gtggcgaagt
    21961 cctcggccat ctggcgcacc gcacccgggg tggcgtcggg tgggaaggcc cggctgcggc
    22021 cgtggtggtg ggcggccagc gccatcgtgg gcacgaggtc gacccacggg aggacgtcgt
    22081 agtcggaggg gtcgaggagg gcgaactcca gggccggaga gatgaggatc gtcccgttga
    22141 gcccgatgcc ccgctcgttg gcgagccggg cgagcttggc cgcccggaag ccgccgtagg
    22201 actcgccggc gacgaacacg ggtgacgccc accggtcgtg ccccgacagc cagcgggcca
    22261 tcagctcggt gagggagtcc aggtccttgt tgacgccgaa gaacgccttg gggtcgcgac
    22321 cttcgccgcc gccgcccttg ccgccaccgt tgccactgtg gtcgcccttg tcgccgccgc
    22381 ccttggccgc gtcgtccttg acgatgcggc tccaaccggt gccgaccggg tcgacgaaga
    22441 cgaggtcggt gaacgcaagc cacgactcgg cgttgtcgac gagcgccgtc ggcggtgcga
    22501 gcggcatgcc ccgctcgtcg aatcccaccc ggcgggggcc gagcgccccg acgtgcaggt
    22561 acgccgacga cgctccgggg ccgccgttga acacgaaggt gaccgggcgg gcggcgccgg
    22621 cgtcgtcgac gaggtaggag accgagaaga tgtcggcgat cggctcgtcg tcgtcatcgc
    22681 gcagggccat ccacgtggcc tcggccgtgt agggcaggct gccgccgtcg ggggtcgtcc
    22741 acgccgcagc ggtggtggcg cccttcggca gcttgggctt cgcggcgtcg tcgttcgtcg
    22801 gcttctcgtc ggacatggcg ggcacgctaa cgaggccggc ccgcaccccc gacccgacgt
    22861 cggacggcgg ttcagccgat gacctcgccg tacatctcgc cgagcgggtc cgagatgagc
    22921 tcgatgcgct cgccgtcggg gccacggaag tacaacgacg agccgctctc gtggtggtac
    22981 tcgacgccgg cctcgtcgag cttgcccttg aggcgctccc atcggagggg atcgacggag
    23041 atggcgaggt ggtggaggcc acccagcacc tcggcgtacc gcccgaggtc gagcccgggg
    23101 aagtcgaaga aggcaagggc gttgccggcg ccgatgtcga agaagaagtg ggtggaaccg
    23161 cggtagtcgc ggttctcgaa cagctcgacg agcgggaact cgagcaagcc cgagtagaac
    23221 tcgatggtcc gctcgaggtc gccggagatc agcgccacgt ggtgcacgcc tcgggcggag
    23281 gacatgcgcc gtcccgggcc cgcctccagg tgccgctcgc gcagccgttg ccactcggag
    23341 gtccggtcca cggcgtcgcc gtggtcggtc tcggcggcgc gcatggcgtt cggggaagcg
    23401 gtgccggcgc tgccggcttc gggcacgtcg gtcatcgccg gacctccagg tcggactccc
    23461 agacgatgac ctcggaccgc tcggtcgcag tgaccaccgg gtgcccggcg tcccgcagcc
    23521 gagcggcgtc accggcggcg agggccagcg gctcgcccgg ggcgccgtcg gtctggctca
    23581 gctccacggc accgcgcgcc acgaacaggt ggacgaaggg agcggcgggc aggtccaccg
    23641 accgtccggc ctccagccgg gcgacccaca tggcggcgtg ctgctggtgg atggagatgg
    23701 cgctgtcgtg gcctcggccg gacgcgaccg gcaccaggcc gccggcgtcg agctcggcgt
    23761 tcaggtcgag ctgctcgtac ccggccgtga tcccgggctc gtggggtggc acccacatct
    23821 gcacgaagtg cacgtcggcc gtggtcgagt ggttcatctc ggagtgcatg atgccgcgtc
    23881 cggcgctcat ccgctgggcc aagccgggat agatcacgcc ctcgttgccc tcggagtccc
    23941 ggtgctcgag ctcgccgtcg aggacccagg tgacgatctc catgtcgcgg tgggggtgcg
    24001 tggagaagcc tccaccggcg gcgacccggt cgtcgttgga cacgatgagc aggccgtggc
    24061 cgacctcgga cgggtcgtag tggccgccga agctgaagct gtggcgcgag tcgagccagg
    24121 agatccgggt gtggaaccgc tcgtcggctc ggcgcagctc ggtggtcggg atgttcatgg
    24181 cgcacctctc ggtctggtcg gcgtcatccc ctccaacagt agttgcacgc gcaacattcc
    24241 cgccgcccgg accaaccggg accttggacc ctgttgcggc cgggccgcgc cgctgacgat
    24301 gtgccgacac cacgcaccga cgcgaccagg gggacacccg atgcggatca tgctgaccga
    24361 ccgctccgac acgagtacca cggtggccga cggcctccgg cgcgatggcc acgagatcgt
    24421 ggacctgtgc ctgcccgatc ccgcctcccc gctcacgtgc cggggcctgg tcgagcaccg
    24481 ctgcccgctc gacgaagggg cggatctggc catcacc
//
LOCUS       DATISU010000287         7716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_18540, whole genome shotgun sequence.
ACCESSION   DATISU010000287 DATISU010000000
VERSION     DATISU010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18540"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VLR27_11550"
     CDS             complement(<1..464)
                     /locus_tag="VLR27_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSP04132.1"
                     /translation="MADLIRQTIRLGAPPWPTVDPFLFCVHHVDAYPAGNLHLGPDAE
                     LQGRAIGQDFAGVDGWNMYHGSVVPGFPSHPHRGFETITYVRRGLVDHADSLGAAARY
                     GKGDVQWMTAGKGIQQAEMLTLLDQSGPNPMELFQIWLNLPAEDKMVEPSYK"
     gene            complement(505..2586)
                     /locus_tag="VLR27_11555"
     CDS             complement(505..2586)
                     /locus_tag="VLR27_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSP04133.1"
                     /translation="MFFEVQPGSDAGDRRYRELVEQLDMPMLIHVDGTIVFATQAVVD
                     LVRVGTLDELMGRQLVDFIHPDDLELVFDHMLRVLAGEPSERHRYRIRRADGTDAWVE
                     AAATPYLLGDVAAALVVVEDITGKLEAEAKLRASEVRYRALVQNSADIVAIVSAAGVL
                     TYASPATAAVLGREVDEIAGTDVRHWLHPDDTIVIERLIGRLRHGPLGEGTSATFRVR
                     HQDGTWRHLEAIATSRLDEPAIGGVVVNARDVTDRIAAAARLRRMALHDSLTGLANRT
                     LIEDRIKQAVQLGPTAVVMLDLNRFKDINDSLVHAVGDVVLAEVADRIRRSVRSGDTV
                     GRVGGDEFVLVLPQAGRMEDISGTVERIMGTLGEPVEHGTSRFRLSASVGIAFAPEHG
                     TTGEGLLRAADAAMYRAKRERSGPAVYDHRDREQSIERLALSSELVDAIELGQFELHY
                     QPKYDLASGRMTGAEALLRWFHPERGLIPPDGFLPLAEDTGAIHAITRWVVDTAVAQA
                     RVWAEQGHDLSVAVNLSPSSLQDPAFGDHVQSVLVAEGVPSERLVLEVTEQTVMADPE
                     TALRVMRRLSEIGVRFSVDDFGTGYSSLTYLRMLPVTELKIDRSFIGDDCGEVDHDIV
                     ETIIALSRRLGLQVVAEGIESCDATDYLRSAGCDVGQGFHLGHPVPADELDLDHAACA
                     VHTREDVHLAS"
     gene            complement(2730..4535)
                     /locus_tag="VLR27_11560"
     CDS             complement(2730..4535)
                     /locus_tag="VLR27_11560"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HSP04134.1"
                     /translation="MSTTAPVATIGPDATVAEAAAEMHRRSASALVVLEDGRLVGLLT
                     ERDLVRVIVDGADPAEVRVRQRMRMDVVTVAPGTDAMEARELMGRHALRHLPVVEDGR
                     VVGLIEQTEPRTGLNFRERREVPERGTLPEPVADFRGPYADGPPADELRVDAPDLSVL
                     GFAEVRRGVVIWWTLVWGVVRALLRRRWAVIRRRDAEGWWDAASEGAVDAFEKLGPAF
                     VKLGQIMASSPGVFPEPLADACQRTLDEVPPFDSATARQIVEEDLGRPPEELFRFFDD
                     KPLASASIGQVHACVLPDGREAVLKLQRPGIRALFTADLRIMWRIARLAEKWDWLSSV
                     DPVAIIEDSAEINFQELVPALEAKRQAEFRDMIGFFGDNENITAPEVYWDYCGPRTIC
                     MQRMTGTPLDEFEAMARQGIDGELMLRWGAKVWIESVCVHGPFHGDMHAGNIWFLPDG
                     RASFLDFGIMGELTPEYREVIKDILYTCMIDGDFTRVARAYRQVGVFPDDVGSDEELG
                     MRLSMILGPMLQAGIGGMNLGEMMKMSIDLMDSYGAKGPRELVLIAKQLAYMERYAKG
                     MAPDYRIIQDLYLIKNIFPEAVAAKAAELGIEVPA"
     gene            complement(4532..5386)
                     /gene="lgt"
                     /locus_tag="VLR27_11565"
     CDS             complement(4532..5386)
                     /gene="lgt"
                     /locus_tag="VLR27_11565"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HSP04135.1"
                     /translation="MLTVLASLPSPPDNAISIGPLELRAYGLFIAIGAIVAVGIARRR
                     WASLTGSDSDAIGDVAVWAIPAGFVGARLYHVITDWHRFEDAPLDAFKVWEGGLGIPG
                     AILLGAVVGVAVARRKGYDVPLLMDAVAPALPVAQAIGRLGNWFNQELFGRPTDLPWG
                     LEIDPDRRPEGYETVETFHPTFLYEALWNLALAAFLVVLGRKKVLRPGQLFVLYVGGY
                     ALGRLWVEALRIDTATRIAGVRVNLWVSGLTIVAAAVWFLWWQRRPERGAEVESQDTG
                     SDAAKDER"
     gene            complement(5386..6276)
                     /locus_tag="VLR27_11570"
     CDS             complement(5386..6276)
                     /locus_tag="VLR27_11570"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HSP04136.1"
                     /translation="MDDAGAVPMDHDARVAADEDFLGLREVGSGRFTFEVSSRLARAD
                     ERLYGGAAVALSVAAAAAETGRDALWCTTQFVSTAMLGSTVDLQVEVLAAGRRTSQVR
                     VTATTDDGVVFASLGACGVLKPDGLAGTFEERPEVSPPGEGALGFQPSGRDGESGWHT
                     AVEVRTAEIRRHPDPGPGRVCLWLRRSDGGPVTPALAGYLADLVPLSIVRGCGVTGAG
                     TSLDNTIRIGAAAETEWILLDLRPNLAVGSYGHGIANVWTEDGHLLAVASQTASLMEL
                     DFDPLTAFGSHASELQERNR"
     gene            6344..7003
                     /locus_tag="VLR27_11575"
     CDS             6344..7003
                     /locus_tag="VLR27_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04137.1"
                     /translation="MVPDDDRSTRDDVLAVVRAVAGKGVELAGSGAGALGSGVDEALR
                     ALVDRRVERALRPSAPAVSATELVDALGTNGSSVSPWLGAGAARLARSGRAARFVGGR
                     TPIGLAVRFGPALYQAVSANLQGLDAAIAHLVTRARDHRIDPDPTRLRTAVVQALTGD
                     PVDPDTEADPSALVRVWLGDAGRRIVPFGFGRINGLNRGRTPDAVAAALASLDVRRLR
                     R"
     gene            7008..>7716
                     /locus_tag="VLR27_11580"
     CDS             7008..>7716
                     /locus_tag="VLR27_11580"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04138.1"
                     /translation="MLTKPDKVLIPAADGHEAVTKADVYDYATRIADRMLPYIADRPA
                     VLQRFPDGTDGEGWYQKHVPKHFPDWIPTVELPKRGGTVRHVVIDSVEALQYVVNQGT
                     ISIHVLGGTVSAPDVPAELLIDLDPSIDDPAQVVEATAAVRSLLDDLDVTGFVKTSGS
                     RGIHVHLPLRGDARFDAVRALSNRLAAILAERHPDLLTVEFAKDDRGDRLFVDVLRNA
                     PAQHAVAPYTLRPKPGAP"
BASE COUNT         1068 a         2821 c         2747 g         1080 t
ORIGIN      
        1 atcttgtagc tgggctcgac catcttgtcc tcggccggca ggttcagcca gatctggaac
       61 agctccatcg ggttgggccc ggactggtcg agcagcgtta acatctcggc ttgctggatg
      121 cccttgccgg cggtcatcca ctgcacgtcg cccttgccgt agcgggcggc ggcgccgagg
      181 gagtcggcgt ggtcgaccag gccgcgtcgc acgtaggtga tggtctcgaa gccccgatgc
      241 gggtgcgagg ggaacccggg cacgacggag ccgtggtaca tgttccagcc gtcgacgccg
      301 gcgaagtcct ggccgatggc ccgaccctgg agctcggcgt cgggaccgag gtgcaggttg
      361 cccgccgggt aggcgtcgac gtggtggacg cagaacagga acggatcgac cgtgggccac
      421 ggtggcgcgc ccaggcggat ggtctgtcgg atcaggtcgg ccatgcctcc aggctaggga
      481 gatcggcccg tcgtgcgtcc gcgctcacga ggcgaggtgg acgtcctccc gggtgtggac
      541 agcgcaggcg gcgtggtcga ggtcgagctc gtccgccggc acggggtgcc cgaggtggaa
      601 gccctgcccc acgtcgcacc cggcggagcg caggtagtcg gtcgcatcgc agctctcgat
      661 gccctcggcc acgacctgca ggccgagccg gcggctcagg gcgatgatcg tctcgacgat
      721 gtcgtgatcg acctcgccgc agtcgtcgcc gatgaacgac cggtcgatct tcagctcggt
      781 cacgggcagc atccgcaggt aggtcagcga gctgtacccg gtgccgaagt cgtcgaccga
      841 gaaccgcacg ccgatctcgg acagccggcg catgacgcgc agcgccgtct ccggatcggc
      901 catcacggtc tgctcggtga cctcgagcac cagccgctcg ctcggcaccc cctcggccac
      961 caggacggac tgcacgtggt cgccgaatgc ggggtcctgc aggctcgacg gcgacaggtt
     1021 gaccgccacc gacaggtcgt ggccctgctc cgcccacacc cgtgcttggg ccaccgccgt
     1081 gtccacgacc cagcgcgtga tggcgtggat ggcgccggtg tcctcggcca gcggaaggaa
     1141 cccgtcgggc gggatcagcc ctcgctccgg gtggaaccac cgcagcagcg cctccgcgcc
     1201 ggtcatccgc ccgctcgcca ggtcgtactt cggctggtag tgcagctcga actggcccag
     1261 ctcgatggcg tcgaccagct ccgagctgag cgccagccgc tcgatgctct gctcccggtc
     1321 gcggtggtcg tacaccgccg gaccggaccg ctcccgcttg gcccggtaca tggcggcgtc
     1381 cgcggcgcgc agcagccctt cgccggtggt cccgtgctcc ggcgcgaagg cgatgccgac
     1441 ggacgcggag agccggaagc ggctggtccc gtgctcgacg ggttccccca gggtccccat
     1501 gatgcgctcc acggtgccgc tgatgtcctc catccgaccg gcctggggca ggacgaggac
     1561 gaactcgtca ccgccgacgc ggccgacggt gtcgcccgag cgcaccgagc gacggatccg
     1621 gtcggcgacc tcggcgagca cgacgtcgcc gacagcgtgg acgagcgagt cgttgatgtc
     1681 cttgaaccgg ttcaggtcca gcatgaccac ggccgtcggg ccgagctgga cggcctgctt
     1741 gatgcggtcc tcgatcaggg tgcggttggc cagcccggtg agggagtcgt gcagcgccat
     1801 gcgtcgcagg cgcgccgccg ccgcgatccg gtcggtgacg tcgcgggcgt tgaccaccac
     1861 gccgccgatg gccggttcgt cgaggcgcga ggtggcgatg gcctccaggt gccgccaggt
     1921 cccgtcctgg tgccggaccc ggaaggtggc gctcgtcccc tcccccagcg gcccgtggcg
     1981 gagccggccg atcaggcgct cgatcacgat ggtgtcgtcg ggatggagcc agtggcgcac
     2041 gtcggtgccg gcgatctcgt cgacctcccg accgaggacc gcagcggtcg ccgggctggc
     2101 gtaggtgagg acccccgcgg ccgacacgat ggccacgatg tcggcgctgt tctgcacgag
     2161 cgcccggtag cggacctccg aggcacgcag cttggcctcg gcctcgagct tgccggtgat
     2221 gtcctccacc accacgaggg cagcggccac gtcgccgagg aggtacggcg tggcggccgc
     2281 ctccacccag gcgtcggtcc cgtcggctcg gcggatccgg tagcggtggc gctccgaggg
     2341 ctcaccggcg aggacccgca gcatgtggtc gaagaccagc tcgaggtcgt cggggtggat
     2401 gaagtccacc agctgccggc ccatcagctc gtccagggtc ccgacccgca ccaggtccac
     2461 gacggcttgc gtggcgaaca cgatggtgcc gtccacgtgg atgagcatcg gcatgtcgag
     2521 ctgctcgacc agctcccggt accgccgatc tcctgcatca ctgcccggct gcacttcgaa
     2581 gaacatcgcg atccgccaat cgggcgccgt cccggctcgg tgccggtcct gttgctgcgc
     2641 tgtgctgtgc atcggcacga tgcgcgaccg actggatgtg acgtccgcac gtcctggcat
     2701 ggtccgtcgt gaccatgccg gtggtcagct cacgccggga cctcgatgcc gagctcggcc
     2761 gccttggctg ccaccgcctc ggggaagatg ttcttgatca ggtacaggtc ctggatgatg
     2821 cggtagtccg gggccatgcc cttcgcgtag cgctccatgt aggccagctg cttggcgatc
     2881 agcaccagct cgcgcggccc cttggcgccg tagctgtcca tcaggtcgat gctcatcttc
     2941 atcatctcgc ccaggttcat cccgccgatg ccggcctgga gcatggggcc gaggatcatc
     3001 gacagccgca ttccgagctc ctcgtcggag ccgacgtcgt cggggaacac gcccacctgg
     3061 cggtatgccc gggcgacccg ggtgaagtcc ccgtcgatca tgcaggtgta gaggatgtcc
     3121 ttgatcacct cccggtactc gggggtgagc tcgcccatga tgccgaagtc gaggaagctg
     3181 gcccggccgt cgggcaggaa ccagatgttg ccggcgtgca tgtcgccgtg gaacgggccg
     3241 tgcacgcaca ccgactcgat ccagaccttg gcgccccagc gcagcatcag ctcgccgtcg
     3301 atgccctggc gggccatggc ctcgaactcg tcgagcgggg tgccggtcat gcgctgcatg
     3361 cagatggtcc gcggcccgca gtagtcccag tacacctcgg gggcggtgat gttctcgttg
     3421 tcgccgaaga acccgatcat gtcccggaac tccgcctggc gcttcgcctc gagcgcgggc
     3481 acgagctcct ggaagttgat ctcggccgag tcctcgatga tcgccaccgg gtcgaccgac
     3541 gacagccagt cccacttctc cgccagccgg gcgatgcgcc acatgatccg caggtcggcg
     3601 gtgaacagcg cccggatgcc ggggcgctgc agcttgagca cggcctcccg gccgtcgggc
     3661 agcacgcagg cgtgcacctg cccgatggag gccgaggcca ggggcttgtc gtcgaagaag
     3721 cggaacagct cctcgggcgg gcggcccagg tcctcctcca cgatctggcg ggccgtggcg
     3781 ctgtcgaagg gcggcacctc gtcgagggtg cgctggcagg cgtcggccag gggctcgggg
     3841 aacacgcccg gtgaggacgc catgatctgg ccgagcttca cgaacgccgg gccgagcttc
     3901 tcgaacgcgt cgaccgcgcc ctcggaggcg gcgtcccacc agccctcggc gtcgcgccgg
     3961 cggatgacgg cccagcggcg ccggagcagg gcgcgcacga cgccccacac gagcgtccac
     4021 cagatcacga cgccccggcg gacctcggcg aagcccagca cggacaggtc gggggcgtcc
     4081 acgcgcagct cgtcggccgg aggaccgtcg gcgtaggggc cgcggaagtc ggcgacgggc
     4141 tcgggcaggg tgccccgctc gggcacctcc cggcgctcgc ggaagttgag gccggtgcgg
     4201 ggctcggtct gctcgatgag gccgacgacc ctgccgtcct ccaccacggg gaggtgacgg
     4261 agggcgtggc gacccatgag ctcgcgcgcc tccatcgcat ccgtgcccgg cgcgaccgtg
     4321 acgacgtcca tccgcatgcg ctggcggacc cgcacctcgg cggggtcggc accgtcgacg
     4381 atgacgcgca ccaggtcgcg ctcggtgagc aggccgacga ggcggccgtc ctccagcacc
     4441 acgagcgctg acgcgctgcg gcggtgcatc tcggcggctg cctcggcgac ggtggcgtca
     4501 ggcccgatcg tggcgaccgg tgcggtggtg ctcatcgctc gtccttcgcc gcgtcggacc
     4561 ccgtgtcctg gctctccacc tccgcacccc gctccgggcg gcgctgccac cacaggaacc
     4621 acacggccgc cgccacgatc gtgaggccgc tcacccagag gttgacccgc accccggcga
     4681 tgcgggtggc ggtgtcgatc cgcagcgcct ccacccagag gcgcccgagc gcgtacccgc
     4741 cgacgtagag cacgaagagc tggccggggc ggaggacctt cttgcgaccg agcaccacga
     4801 ggaacgcggc gagggccagg ttccacagcg cctcgtacag gaaggtgggg tggaaggtct
     4861 cgaccgtctc gtagccctcc gggcggcggt cggggtcgat ctccagtccc cacggcaggt
     4921 cggtgggacg accgaacagc tcctggttga accagttgcc gaggcgaccg atcgcctggg
     4981 cgaccggcag ggccggggcg acggcgtcca tgaggagggg cacgtcgtag cccttgcggc
     5041 gggcgacggc gacgccgacc acggccccga ggaggatggc ccccgggatg ccgaggccgc
     5101 cctcccagac cttgaaggcg tcgaggggcg catcctcgaa gcggtgccag tcggtgatga
     5161 cgtggtacag ccgggccccg acgaagccgg ccgggatggc ccagacggcg acgtcgccga
     5221 tggcgtcgct gtcgctgccg gtgagcgacg cccaccgccg gcgggcgatc cccaccgcca
     5281 cgatcgcccc gatggcgatg aacaagccgt aggcccgcag ctccagcggg ccgatggaga
     5341 tcgcgttgtc cggcgggctc gggagggagg cgaggacggt gagcatcacc ggttgcgctc
     5401 ctgcagctcg gaggcgtgcg agccgaaggc ggtgaggggg tcgaagtcca gctccatcag
     5461 cgacgcagtc tggctcgcga cggccaggag gtgtccgtcc tcggtccaga cgttggcgat
     5521 cccgtgcccg tagctgccca ccgccaggtt cggtcgcagg tcgagcagga tccactcggt
     5581 ctcggcggcc gccccgatgc ggatggtgtt gtcgaggctg gtgccggcgc cggtcacccc
     5641 gcagccccgg acgatggaga ggggcacgag gtcggcgagg tacccggcga gggcgggcgt
     5701 caccggtccg ccgtcgctgc gacgcagcca caggcacacc cgaccgggcc ccgggtcggg
     5761 gtggcggcgg atctcggcgg tgcggacctc gacggcggtg tgccagcccg attcaccgtc
     5821 ccggccgctg ggctggaacc ccagcgcccc ctccccagga gggctgacct ctggacgttc
     5881 ctcgaaggtg ccggccagcc cgtcgggctt gagcacgccg cacgcgccga gggacgcgaa
     5941 caccacgccg tcgtcggtgg tggccgtcac ccggacctgg ctcgtccggc gaccggcggc
     6001 cagcacctcc acctgcaggt ccacggtgct cccgagcatc gccgtcgaca cgaactgggt
     6061 cgtgcaccac agcgcatccc gtccggtctc cgcggccgcg gcggccaccg agagggcgac
     6121 ggcggctccg ccgtagaggc gctcgtcggc acgggccagg cgggagctga cctcgaacgt
     6181 gaaccgcccc gatccgacct cccggagccc gaggaagtcc tcgtcggcgg cgacccgggc
     6241 gtcgtggtcc atcggcacgg caccagcatc gtccatgtcc cgaggtgcgc cacaaacggc
     6301 gcgtcccgcc gcgttctgcc ggcgcgcccg tagccttccg gggatggtcc ccgacgacga
     6361 ccgctccacc cgagacgacg tcctggccgt ggtccgcgcc gtggccggga agggcgtcga
     6421 gctcgccggg agcggtgccg gcgccctcgg gagcggggtc gacgaggccc tgcgggccct
     6481 ggtcgaccgg cgggtcgagc gtgcgctgcg accgtcggcc ccggccgtgt cggccaccga
     6541 gctggtggac gccctcggca cgaacggctc ctccgtctcg ccctggctgg gcgcgggcgc
     6601 cgctcgtctc gcccgctccg ggcgtgccgc ccgcttcgtg ggagggcgca ccccgatcgg
     6661 cctcgccgtg cggttcggac cggcgctcta ccaggcggtc agcgccaacc tgcaggggct
     6721 ggacgccgcc atcgcccacc tcgtcacccg ggcccgggac caccggatcg accccgatcc
     6781 cacccgcctg cgcaccgccg tggtgcaggc cctcaccggc gacccggtcg atcccgacac
     6841 cgaggccgac ccgtccgccc tcgtgcgcgt gtggctcggc gacgccgggc ggcgcatcgt
     6901 ccccttcggg ttcggtcgca tcaacgggct gaaccggggc cgcacccccg atgccgtcgc
     6961 cgccgcgctc gcgtccctcg acgtgcgccg cctgcgccgc tgagccgatg ctcaccaagc
     7021 ccgacaaggt cctgatcccg gccgccgacg ggcacgaggc ggtcaccaag gccgacgtct
     7081 acgactacgc cacccgcatc gccgaccgga tgctccccta catcgccgac cgccccgccg
     7141 tgctgcagcg cttccccgac ggcaccgatg gcgagggctg gtaccagaag cacgtcccga
     7201 agcacttccc cgactggatc cccaccgtcg agctgcccaa gcgcggcggc accgtccgcc
     7261 acgtcgtcat cgactcggtc gaggcgctgc agtacgtggt gaaccagggc accatctcga
     7321 tccacgtcct cggcggcacg gtctcggcac ccgacgtccc cgccgagctc ctgatcgacc
     7381 tcgacccctc catcgacgac ccggcccagg tggtggaggc gacggccgcg gtgcggtccc
     7441 tgctcgacga cctcgacgtc accgggttcg tcaagaccag cgggtcgcgt ggcatccacg
     7501 tgcacctgcc cttgcggggc gatgcccgct tcgacgcggt gcgggccctg tcgaaccgcc
     7561 tcgccgcgat cctggccgag cgccaccccg acctgctcac ggtggagttc gccaaggacg
     7621 accgcggcga tcggctcttc gtcgacgtgc tccgcaacgc ccccgcccag cacgccgtgg
     7681 cgccctacac gctgcgaccc aagccgggcg cgccgg
//
LOCUS       DATISU010000288         1904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_205084, whole genome shotgun sequence.
ACCESSION   DATISU010000288 DATISU010000000
VERSION     DATISU010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205084"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /gene="infB"
                     /locus_tag="VLR27_11585"
     CDS             complement(<1..1376)
                     /gene="infB"
                     /locus_tag="VLR27_11585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HSP04139.1"
                     /translation="MQAPTIGGVRVRKGDGETVRLARGSSLTDFAEKINVDAAALVQM
                     LFSLGEMVTATESVNDETFELLAEELNYVVEVVSPEDEDRELLESFDLDFGADEGDES
                     DLAFRPPVVTVMGHVDHGKTKLLDALRSANVVDKEAGGITQHIGAYQVTTDVDGNERR
                     ITFIDTPGHEAFTAMRARGAQATDIAILVVAADDGVMPQTIEALNHAKAAGVPVVVAV
                     NTIDKPEADPTKVRGQLTEYGLIPEEYGGDSMFVDVSAKSELNLDKLLEAVVLTADAS
                     LDLRANPDQDAQGLVVEAHLDRGRGPVATVLVQRGTLRVGDSIVAGAGYGRVRAMLDE
                     HGDNITEADPSRPAMVLGLTAVPGAGQNFIVVDDDRMARQIAEKRESRERAAMQAKRR
                     VRRTLEDFMASMEKGESQELNLILKGDVSGSVEALEDALAQIDVGEEVNLRVIDRGVG
                     AITETNVDL"
BASE COUNT          258 a          670 c          702 g          274 t
ORIGIN      
        1 aggtcgacgt tggtctcggt gatcgcaccg acaccgcggt cgatgacccg caggttgacc
       61 tcttcgccga cgtcgatctg ggcgagggcg tcctcgagtg cctcgaccga accggacacg
      121 tcgcccttga ggatcaggtt gagctcctgg ctctcgccct tctccatgga ggccatgaag
      181 tcctcgaggg tacgacgcac gcgacgcttg gcctgcatgg ccgcacgctc acgcgactcg
      241 cgcttctcgg cgatctggcg ggccatgcgg tcgtcgtcga ccacgatgaa gttctggccg
      301 gcaccgggca ccgcggtcag accgaggacc atcgcgggac gcgacgggtc ggcctcggtg
      361 atgttgtcac cgtgctcgtc gagcatcgcc cggacccggc cgtaaccggc tccggcgacg
      421 atcgagtcac ccacgcgcag cgtgccgcgc tggaccagca ccgtggcgac cggaccgcga
      481 ccgcggtcca ggtgcgcctc gacgacgagg ccctgggcgt cctggtcggg gttggcccgc
      541 aggtccaggg acgcgtcggc ggtgagcacg acggcctcga gcagcttgtc gaggttgagc
      601 tccgacttgg ccgagacgtc gacgaacatc gagtcgccgc cgtactcctc ggggatcagg
      661 ccgtactcgg tcagctggcc gcggaccttg gtcgggtccg cctcgggctt gtcgatcgtg
      721 ttgaccgcga cgacgaccgg gacaccggcc gccttggcgt ggttgagcgc ctcgatggtc
      781 tgcggcatca caccgtcgtc ggccgccacc acgaggatcg cgatgtcggt cgcctgggca
      841 ccacgggcac gcatggcggt gaacgcctcg tgaccggggg tgtcgatgaa ggtgatccgg
      901 cgctcgttgc cgtcgacgtc ggtcgtgacc tggtaggcac cgatgtgctg cgtgatgcca
      961 ccggcctcct tgtcgacgac gttggcgctg cgcagcgcgt cgaggagctt ggtctttccg
     1021 tggtcgacgt gacccatgac cgtgacgacc ggcgggcgga acgcgagatc gctctcgtca
     1081 ccctcgtcgg caccgaagtc caggtcgaag gactcgagca gctcgcggtc ctcgtcctcg
     1141 ggggacacga cctcgacgac gtagttgagc tcctcggcga gcagctcgaa cgtctcgtcg
     1201 ttgacggact cggtcgcggt gaccatctcg ccgagcgaga agagcatctg caccagtgct
     1261 gcggcgtcga cgttgatctt ctcggcgaag tcggtcagcg acgaaccgcg cgccaggcgc
     1321 accgtctcgc cgtcgccctt gcggacgcgg acgccaccga tggtgggcgc ctgcatctgc
     1381 tcgaactctt gacgacgagc gcgcttcgac ttgcgaccac ggcgcgacgg gccaccgggg
     1441 cgaccgaacg caccctgggt ctggccacgc tggccgggac gggcacgctg gccgctggcg
     1501 ttgggcccga agctgggacc gccaccggga cgacccggag cgccgcctcc accggggcca
     1561 ccacgggcac cgccggggcc accacgggcg ccgccggggc caccacgggc gccgccgggg
     1621 ccaccacggg caccgccggg gccgccgggg ccaccacggg caccgccggg gccaccggga
     1681 ccggagccga aggcggccgg ggacttgggc atcatcgcgg ggttcggacg gggcatgccc
     1741 ggccgacccg gggcaccacc gtcacgcgcg gctgccggcc gcggcggacg ctggtcgccg
     1801 gcacccgtgc cggcgccggc cgccgggacc ggggcctcgg ggttcggagc gggcgcgggc
     1861 cgacggccca tgccctggct gggggcgaag gggtggttgc cggg
//
LOCUS       DATISU010000289        11596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_298352, whole genome shotgun sequence.
ACCESSION   DATISU010000289 DATISU010000000
VERSION     DATISU010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 11596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11596
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298352"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /locus_tag="VLR27_11590"
     CDS             <1..1433
                     /locus_tag="VLR27_11590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP04140.1"
                     /translation="HERSVGDMAERAADITAATYPEAADWTDAQRNRFVRQARARFEA
                     IVALTRAGSADDELLEDLADAGGAAAFAGAPLPQVLLTLRISRDLLVQSAVAAAAELG
                     QQRSVALAVVLTRVLPVLDRLTDTVARGDWAAVRMREQEVLARYAHMVEHAGHGVYEV
                     ELDGTVCFANSMLARITGHHLDDLLGRDLAVSLPPADPTPDIDVYRSPTVSGWRPLRV
                     LRADGVERELLLQVTERTHQGRPVGYDGIVRDVTPERALERQKNDFLALVTDELRQPL
                     ATVLGLGVTLASEADELSCEQVASMGRTIHVQSDRIARLADDLHDVSRIRADALSLMM
                     RRVDIAPACAAAIRMLPGAEAVEVDVPADLEVIADGRRLQHVIAHLVENGLRHGRPPV
                     VLRARAVDGAIQIEVLDHGDGVPLHQVEGLFASLVPSAADDRLRDRPSGLGLPLARSL
                     VEAMGGRIRHDRLPDGATRFRVTLPT"
     gene            complement(1738..2934)
                     /locus_tag="VLR27_11595"
     CDS             complement(1738..2934)
                     /locus_tag="VLR27_11595"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HSP04141.1"
                     /translation="MSSPAVTVSPETVTTMLRGVIDPELGSDVVELGMVRGVDVAPSP
                     EGEGVTVTVTIALTTSGCPLRAQIQKDVRTRILSMEGVDAVKLAWDEMNQEEKARAME
                     RARFNVAQREEDSSVGPNTKVVLVSSGKGGVGKSTVTVNLAAAMAATGLKVGVLDADI
                     WGFSVPRLLGVSGRLASREIDGRKMMVPQERELGEGLLRIVSMGFLVEDEQSALMWRG
                     LMLNRGVQHFLQDVDWGDDLDYLLVDMPPGTGDVQMGVAKLIPRSEVLVVTTPAVNAQ
                     KVAARAVSMSRKNYLRVCGIVENMSAFVCDHGERYELFGSGGGEILAQESGAPLLGEI
                     PMEPTVSLEGDAGEPVALRDGPAGEAFRALATRLVEEAVPQSEMAGCSARMLDAAMAN
                     LDALEL"
     gene            3016..3681
                     /locus_tag="VLR27_11600"
     CDS             3016..3681
                     /locus_tag="VLR27_11600"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP04142.1"
                     /translation="MAERLEILKTLGDNTRYAIYLELARSVRPLATAEIAETLDLHAN
                     TVRPHLERMRDLGLVEVTTVATGSVGRPQHRYALAADAPSLGLEPPAFPTVTRMLLRA
                     AAAAGVDPEELVEAGRGQGRDDFARTQADGPADLVDALERELEAFGFDPARADDGDEV
                     TIAFTHCPFRELAEAHPDLVCSMHRGMVEGFIGCGAGAEVRRFHPLVDRTPCQVDVVR
                     KAG"
     gene            3832..4149
                     /gene="erpA"
                     /locus_tag="VLR27_11605"
     CDS             3832..4149
                     /gene="erpA"
                     /locus_tag="VLR27_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HSP04143.1"
                     /translation="MITLPATAAAKVTSLIDEEGEAALALRVAVRPGGCSGFSYEMFF
                     DTDVADDDKVLTYGDVRVIVDSSSAVMLEGATLDFKDGLESGFSINNPNAQRTCGCGS
                     SFS"
     gene            complement(4146..4895)
                     /locus_tag="VLR27_11610"
     CDS             complement(4146..4895)
                     /locus_tag="VLR27_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04144.1"
                     /translation="MASEHDRELVARITAGDVRGLLTADDPAAVLDAAGRTEGTVAVD
                     DLAGGIAAARVRLAEVVVARARRAEADQLAAVAPRAPSAADHATPADGRDDDRQAVRF
                     SLAVLLPALVGGAAVYVADGLLLAVVLPAVALAALGVVVLRHRRLPTSDPGATETDPP
                     TVPVEVGDGPAVRAAEAHLRRQQAAWKLVWWEREEPVPDLASWSPRLAATAPITLVSV
                     DVAGRLDADAHAAMTAAAPAAIRVVVLQARG"
     gene            complement(4912..5889)
                     /locus_tag="VLR27_11615"
     CDS             complement(4912..5889)
                     /locus_tag="VLR27_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04145.1"
                     /translation="MRDRLVVPPSRLIVPAALSRRGFLAGVAAAGLLAACGDDDDTGT
                     AATDTTGGSTDEGSSAAGGIVGGLSALRYYGPYYRAGQPARVPFGLADDEGPLTAELM
                     PGELTVSVRDPNTDEVASGLTAQLYTEGLPRPYYAFEFTPETAGFYDIVLDTEVGEVI
                     TQVQIVEADDPTASAMVGPGDQLPALMTPTVDDARGVTPICTREPACDLHARTAADVL
                     AAGEPMVLLVATPAFCQTVICGPVLDVLLGVLPDVPGITAVHAEVFSDPENNPTPPTE
                     ADYAPVIGELGLAFEPVLYTVGADGVVRERLDYIFGEAEIRRALEDLVA"
     gene            5914..7494
                     /locus_tag="VLR27_11620"
     CDS             5914..7494
                     /locus_tag="VLR27_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSP04146.1"
                     /translation="MAGHDQRGPSADPLQLRVNGRQVEVADASASLLGVLRDELGERA
                     PKDGCSPQGQCGCCTVLVDGQPRVACVTPVRRVRGREITTPDHLDDATTWAEALCATG
                     GSQCGFCTPGIVTRLSALRAKDADADHGKVEQALLAHLCRCTGWRTILEAWDAYGADH
                     PPRDLEAAAARATLEGHVPQRLEPEVALGRAGFADDTAPAGSVVAVLDPEGRWTLGES
                     VAAARANAAKVQGRHTTADSEPPIAVPDGDWALTLQTSWVEPAYLETDASWCEPGGEP
                     ASAAGNGGAFGAKADSPVPDAARRLADEHGTTVRALYSREDTVRLGAKRPPLAAGVRA
                     DGTGVVHVARTPGIADAIASVAPGLEVVEVDVPGPPTSANVRGAGWVEAAVLIAGLAG
                     ADGDGWVGPVGQPDGGTARARIAEDGTIQVEADAGEPLDEVALRSYCVGAAHQGASWV
                     TSESLTVDADGQVHDLTIRSFGVLRAVDTPPIHVDIAPGGATPVPVSDAVFAAVAAAV
                     WVHQGRPPVWPTGIPLRT"
     gene            7491..7856
                     /locus_tag="VLR27_11625"
     CDS             7491..7856
                     /locus_tag="VLR27_11625"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSP04147.1"
                     /translation="MTTPVGPYTPVVRSGDWIICSGQVGLADGKLVEGGVREQLTQAM
                     ANVRSLLESQGASLADVVKTTVFLVDMDDYSAMNDVYVQAFDEHADGHRPARSAVAVA
                     ALPIGAQVEIEAWARVPGS"
     gene            7863..8252
                     /locus_tag="VLR27_11630"
     CDS             7863..8252
                     /locus_tag="VLR27_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04148.1"
                     /translation="MAGDEEWAEPVELVGGEESTAEIDSGRPVDWHKVVRTGAAVVAA
                     LSLLWIGRSMADERAQVADDRCMRDVDSVLWRYENAGNRMGRGFGESPIPRDVVDEFI
                     AQARDCGDDLLADALEFEYVTSFEDDD"
     gene            8324..9298
                     /locus_tag="VLR27_11635"
     CDS             8324..9298
                     /locus_tag="VLR27_11635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HSP04149.1"
                     /translation="MRAVLCKQFGPVEDLVVEEVPDPTPGPGEVVVDVAGCGVNFPDV
                     LIIQDKYQFKPELPFSPGGEIAGTVSAVGEGVDGLQVGDAVLATLGWGGMAEKVAVSA
                     ASAIPVPPGVDLVQASGFLLAHGTSHHALVDRAHLQSGETLLVLGAAGGVGLAAVELG
                     ALLGARVIAAASTDEKLELCRAKGAAETINYSTEDLRGRIKELTDGKGIDVCYDPVGG
                     DLSEPALRSMAWDGRFLVVGFAAGDIPKVPLNLPLLKGCSVVGVFWGAFTGKEPERNK
                     ANIAELLGWLAEGKLDPHVSATYPLDQAVQAINDLAERRATGKVVVTP"
     gene            9385..9543
                     /locus_tag="VLR27_11640"
     CDS             9385..9543
                     /locus_tag="VLR27_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04150.1"
                     /translation="MIGAAIIVFVLIVFIPVSVLMSGSLGAAAIGFFLRNEADVRHEG
                     SELVDLNG"
     gene            complement(9533..10804)
                     /locus_tag="VLR27_11645"
     CDS             complement(9533..10804)
                     /locus_tag="VLR27_11645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HSP04151.1"
                     /translation="MLEWYLGPAPPVGLRCWDGSRWGDPDAALQVDVRSPDAVRRLLW
                     DPGELGLARAHVAGELDFDGSVFDLLGLRDRLIDRTADAGLDLTWRERAVLVRDAKRL
                     GVLGRRPEPPPEEARLRGRRHSKGRDRAAISHHYDVGNDFYRLVLGPSMTYSCALFPE
                     RSTTLEDAQALKYEHICRKLGLEPGQRLLDVGCGWGGMVLHAAEHHGVRATGITISEQ
                     QAVLAQERVRAAGLEDRVQIRLQDYRDVADEPYDAVSSIGMFEHVGMEQVRTYFERLQ
                     TLLRPGGRLLNHAISRCSGEGPLDPESFPGRYVFPDGQLHEVGRTVSTMQDLGFECRD
                     VESLREHYARTLRCWVANLEADWDRAVAFVGAPRARVWRLYMAGSAVAFEAHRLNIHQ
                     TLAVQTAADGTSGMPATRPDAVRSLRRLVSR"
     gene            complement(10912..>11596)
                     /locus_tag="VLR27_11650"
     CDS             complement(10912..>11596)
                     /locus_tag="VLR27_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP04152.1"
                     /translation="AMEPLLVYPDPPPPVLSQGLDLAGYPWVAVGADADIERMEPTDG
                     WPGAVILAVTNPEGAFAMCRALRKGDIPLEPVLLIASAEQLPELDMREDLFDDFCVLP
                     FQPAELDARLRNLFHKTGRGARPELVEYGELALNLETYQAAISGKPLDLTYMEYELLK
                     FLASHPGKVFTRETLLSRVWGYEYYGGARTVDVHVRRLRAKLGEEHAGLIQTVRSVGY
                     RFGQSRWMG"
BASE COUNT         1569 a         4334 c         4182 g         1511 t
ORIGIN      
        1 cccacgagcg ctcggtcggc gacatggccg agcgggccgc cgacatcacg gcggccacct
       61 accccgaggc cgccgactgg accgacgccc agcgaaaccg gttcgtccgc caggcgcgcg
      121 cccgcttcga ggccatcgtc gccctcaccc gcgccgggtc cgccgatgac gagctcctcg
      181 aggacctcgc cgacgccggc ggtgccgccg ccttcgccgg cgcacccctg ccccaggtgc
      241 tgctgaccct gcgcatcagc cgggacctgc tggtgcagag cgccgtcgcc gcggccgccg
      301 agcttggcca gcagcgcagc gtcgccctcg ccgtcgtgct cacccgggtg ctgccggtgc
      361 tcgaccgact caccgacacc gtcgctcgcg gcgactgggc cgcggtccgc atgcgggagc
      421 aggaggtcct cgcccggtac gcccacatgg ttgagcacgc cggccacggc gtctacgagg
      481 tcgagctcga cggcacggtc tgcttcgcca actcgatgct ggcccgcatc accggccacc
      541 acctcgacga cctgctcggc cgggacctcg ccgtctccct cccgccggcc gacccgacgc
      601 cggacatcga cgtctaccgc tcccccacgg tgagcgggtg gcggccgctg cgggtgctcc
      661 gcgccgacgg cgtcgagcgc gagctgctgc tccaggtgac cgagcggacc caccagggcc
      721 gccccgtcgg ctacgacggc atcgtccgcg acgtcacgcc cgagcgcgcc ctcgagcgcc
      781 agaagaacga cttcctcgcc ctcgtcaccg acgagctgcg ccaaccgctg gccaccgtcc
      841 tcggcctcgg cgtcaccctc gccagcgagg ccgacgagct gtcgtgcgag caggtggcga
      901 gcatgggccg gaccatccac gtccagtccg accgcatcgc ccgcctcgcc gacgacctcc
      961 acgacgtcag ccggatccgg gccgacgccc tcagcctgat gatgcgccgg gtcgacatcg
     1021 caccggcctg cgccgccgcc atccgcatgc tccccggcgc cgaggcggtg gaggtcgacg
     1081 tcccggccga cctcgaggtc atcgccgacg gtcgccggct ccagcacgtc atcgcccacc
     1141 tggtggagaa cggcctgcgc cacggccgcc cgcccgtcgt cctgcgggcg cgcgcggtgg
     1201 acggggcgat ccagatcgag gtgctcgacc acggcgacgg cgtcccgctg caccaggtcg
     1261 aggggctgtt cgcgtcgctg gtgcccagtg ccgccgacga tcgcctgcgc gaccggccgt
     1321 ccggtctcgg cctgcccctg gcccgcagcc tggtcgaggc gatggggggc cgcatccgtc
     1381 acgatcgcct gcccgacggc gccacccggt tccgggtgac gctgcccacc tgaccggcgt
     1441 ccaccccacg gacggggggt tggcacaaat gacccggccg ggccattccc gccgccacgc
     1501 accgcggtca ggatgggtgc acgccggaca tgccggccac aaccgagggg cgaagcgtcc
     1561 cgagactccc gggtgcgagg gctgttcgca tgcggggttc gccgctcccc ttcgccgctg
     1621 accccgtcga cgtggtgctg gggggcaccg gagcgacggg gtcagccgcg ctccggatcg
     1681 ttctggccta ccgatgtccg ccctggcggg ggtggatgta ggccagaacg ggtcgggtca
     1741 gagctcgagg gcgtcgaggt tggccatggc cgcgtcgagc atgcgggccg agcaaccggc
     1801 catctccgac tgcggcacgg cctcctccac gaggcgggtg gccagggccc ggaaggcctc
     1861 gccggccgga ccgtcgcgca gggcgaccgg ctcgccggcg tcgccctcga gcgagacggt
     1921 gggctccatc gggatctcgc ccagcagggg cgcgccgctc tcctgggcca ggatctcgcc
     1981 gccaccgctg ccgaacagct cgtagcgctc gccgtggtcg cacacgaagg cgctcatgtt
     2041 ctcgacgatg ccgcacaccc gcaggtagtt cttccgcgac atcgacacgg cccgggccgc
     2101 gaccttctgg gcgttgacgg cgggcgtggt cacgaccagc acctcgctgc gggggatgag
     2161 cttggccacg cccatctgga cgtcgccggt gccggggggc atgtcgacga gcaggtagtc
     2221 gaggtcgtcg ccccagtcga cgtcctggag gaagtgctgc acgccgcggt tcagcatcag
     2281 gccgcgccac atcagcgccg actgctcgtc ctcgacgagg aagcccatcg acacgatgcg
     2341 cagcaggccc tcgcccagct cgcgctcctg gggcaccatc atcttgcggc cgtcgatctc
     2401 gcggctggcg aggcgaccgg acacgccgag gaggcgcggg acggagaagc cccagatgtc
     2461 ggcgtcgagg acgccgacct tcaggccggt ggcagccatg gcggcggcga ggttcacggt
     2521 gacggtggac ttgccgacgc cgcccttgcc ggacgacacg agcaccacct tggtgttggg
     2581 accgaccgag gagtcctctt cgcgctgggc cacgttgaag cgggcccgct ccatggcccg
     2641 ggccttctcc tcctggttca tctcgtccca ggccagcttc acggcgtcga ccccctccat
     2701 cgagaggatg cgggtgcgga cgtccttctg gatctgggcg cgcagcgggc acccggaggt
     2761 ggtgagggcg atggtgaccg tgacggtcac gccctcgccc tcgggggacg gggcgacgtc
     2821 gacgccacgg accatgccga gctcgaccac atcgctgccg agctcgggat cgatcacgcc
     2881 gcggagcatc gtcgtgacgg tctcggggga gacggtgacg gcggggctgg acatgggtcg
     2941 agcctaccgc cggtcccggg attctcccgg cgcctcccgt ggaaaccggg acggatgccc
     3001 cggtagcctc gcaccgtggc cgaacgcctc gagatcctga agacgctggg tgacaacacc
     3061 cgctatgcga tctacctgga gctggcgcgc tcggtccgcc ccctggccac ggccgagatc
     3121 gccgagaccc tcgacctcca cgccaacacc gtgcgccccc acctcgagcg catgcgcgac
     3181 ctcggcctgg tcgaggtgac caccgtcgcc accgggtcgg tcggtcgccc ccagcaccgc
     3241 tacgccctgg ccgccgacgc cccctccctc ggcctcgaac cgccggcgtt ccccacggtc
     3301 acgcgcatgc tcctccgagc ggcggccgct gccggcgtcg atcccgagga gctggtcgag
     3361 gcgggccgcg ggcagggtcg ggacgacttc gcccgcaccc aggccgacgg gccggccgat
     3421 ctggtcgacg cgctggagcg ggagctggag gcgttcgggt tcgacccggc ccgggccgac
     3481 gacggtgacg aggtcaccat cgccttcacg cactgcccgt tccgcgagct ggccgaggcc
     3541 caccccgacc tggtctgcag catgcaccgg ggcatggtcg aggggttcat cgggtgcggc
     3601 gccggcgccg aggtccgccg cttccacccc ctggtcgacc gcaccccctg ccaggtcgac
     3661 gtggtccgca aggccggttg agccgagccg caggcgaggc gacccaggtg aacggtgccc
     3721 ttgtggtggc gaagccgcca cgatcgaacg cagggggacg tgacctgtct gggcggtcct
     3781 gagcgcgccg cgcctgcggc acgcccggac acctccgata cgctggtcgc catgatcacg
     3841 ctcccggcca ccgctgccgc caaggtcacg agcctcatcg acgaagaggg cgaggccgcc
     3901 ctggcgctcc gggtggccgt ccgtccgggc ggctgctccg gcttcagcta cgagatgttc
     3961 ttcgacaccg acgtcgccga cgacgacaag gtgctcacct acggcgacgt ccgcgtcatc
     4021 gtggactcct ccagcgccgt gatgctcgag ggcgccaccc tcgacttcaa ggacggcctg
     4081 gagtcgggct tctccatcaa caacccgaac gcgcagcgca cctgcggctg cggctccagc
     4141 ttctcctagc cccgagcctg cagcaccacc acgcggatgg cggccggcgc ggccgccgtc
     4201 atcgcggcgt gcgcatcggc atccagccgc ccggcgacat cgaccgacac gagcgtgatc
     4261 ggcgcggtcg ccgcgagccg aggtgaccag gaggcgaggt ccggcaccgg ttcctcgcgc
     4321 tcccaccaca ccagcttcca cgccgcctgc tggcggcgca ggtgcgcttc ggcggcgcgc
     4381 accgccgggc cgtcgcccac ctcgacgggc accgtcggtg ggtcggtctc cgtcgcgccc
     4441 gggtccgagg tcgggagccg acggtgccgg agcacgacga cgccgagcgc cgcgagcgcg
     4501 acggcgggca gcaccacggc gagcagcagc ccgtcggcga cgtagacggc ggccccgccc
     4561 accagcgccg gcaggagcac cgccagcgag aaccgcacgg cctgccggtc gtcgtcacgc
     4621 ccgtccgcgg gggtggcatg gtcagctgcc gacggggccc gtggggcgac ggcggcgagc
     4681 tggtcggcct cggcccggcg agcccgggcc acgaccacct cggccaggcg aactcgcgcc
     4741 gcagcgatcc cgccggcgag gtcgtcgacc gcgacggtgc cctcggtgcg accggcggcg
     4801 tcgagcaccg ctgccgggtc gtccgcggtg agcaggcccc gaacgtcgcc ggcggtgatc
     4861 cgggcgacca gctcccggtc gtgctcgctg gccatcccgg cagccggtcg ctcaggcgac
     4921 caggtcctcc agggcacggc ggatctcggc ctcgccgaag atgtagtcga ggcgctcccg
     4981 cacgacgccg tcggcgccca ccgtgtagag caccggctcg aacgcaagtc cgagctcgcc
     5041 gatcaccggg gcgtagtcgg cctcggtggg gggcgtcggg ttgttctccg ggtcgctgaa
     5101 gacctcggcg tgcacggccg tgatgccggg gacgtcgggc agcaccccga gcaggacgtc
     5161 gagcaccggt ccgcagatca ccgtctggca gaaggccggg gtcgcgacga gcagcaccat
     5221 cggctcgccg gcagccagca cgtcggcggc ggtgcgcgcg tgcaggtcgc aggccggttc
     5281 gcgggtacag atgggcgtga ccccgcgggc gtcgtcgacg gtcggggtca tgagcgcggg
     5341 cagctggtcg ccggggccga ccatggcgga cgccgtgggg tcgtcggcct ccacgatctg
     5401 cacctgggtg atcacctcgc cgacctcggt gtcgaggacg atgtcgtaga accccgcggt
     5461 ctccggcgtg aactcgaagg cgtagtacgg ccggggcagg ccctcggtgt agagctgggc
     5521 ggtgagcccg gaggcgacct cgtcggtgtt gggatcccgc accgacacgg tgagctcacc
     5581 cggcatcagc tctgccgtga ggggaccctc gtcgtcggcg aggccgaagg ggacgcgtgc
     5641 cggctggccg gcccggtagt agggcccgta gtagcgcagc gccgagagcc cgccgacgat
     5701 gccaccggcc gcgctgctgc cttcgtcggt ggacccgccg gtcgtgtcgg tggcggcggt
     5761 gccggtgtcg tcgtcgtcgc cgcacgcggc caggagaccg gccgcggcga cgccggcgag
     5821 gaagccacga cggctgaggg cggccggcac gatcaggcgg gacgggggga cgacgaggcg
     5881 gtcgcgcatg gagcgcgacc gtagtgtgac cccatggccg gccacgatca gcgaggaccg
     5941 tccgccgacc ccctgcagct gcgggtgaac gggcgtcagg tcgaggtcgc cgacgcctcg
     6001 gcgtcgctgc tcggcgtgct ccgcgacgag ctcggcgagc gggcgcccaa ggacggctgc
     6061 tcaccccagg gccagtgcgg gtgctgcacc gtcctggtcg acggccaacc ccgggtggcg
     6121 tgcgtgaccc cggtgcgccg ggtgcggggc cgggagatca cgacgcccga ccacctcgac
     6181 gacgccacca cctgggccga ggcgctgtgc gccacaggcg ggtcgcagtg cggcttctgc
     6241 acgcccggca tcgtcacccg cctgtccgcg ctgcgcgcca aggacgccga cgccgaccac
     6301 ggcaaggtcg agcaggcgct gctcgcccac ctgtgccgct gcaccggctg gcgcaccatc
     6361 ctcgaggcct gggacgcgta cggcgccgac cacccccctc gcgatctgga ggcggctgcg
     6421 gcgcgggcga cgctggaggg ccacgtcccc cagcgcctcg aacccgaggt cgccctgggc
     6481 cgggccggct tcgccgacga caccgccccc gccggcagcg tcgtcgccgt gctcgacccg
     6541 gagggccgct ggaccctcgg cgagtcggtc gccgccgccc gcgccaacgc ggccaaggtg
     6601 cagggacgcc acaccaccgc cgacagcgaa ccccccatcg ccgtgcccga cggcgactgg
     6661 gcgctcacgc tgcagacgtc gtgggtcgag ccggcgtacc tggagaccga cgcctcgtgg
     6721 tgcgagcccg gcggcgagcc ggccagcgca gccggcaacg gtggggcctt cggcgccaag
     6781 gccgactccc ccgtccccga cgcggcccgc cgcctcgccg acgagcacgg caccaccgtc
     6841 cgggccctgt acagccgtga ggacaccgtg cggctcggcg ccaagcgtcc gccgctggcc
     6901 gcaggggtcc gggccgacgg caccggcgtc gtgcacgtgg cgcgcacccc cggcatcgcc
     6961 gacgccatcg cctcggtggc gcccgggctc gaggtcgtcg aggtcgacgt ccccggtccg
     7021 ccgacgtcgg cgaacgtccg cggcgcgggc tgggtcgagg ccgccgtgct gatcgccggg
     7081 ctcgccggcg ccgacggcga cggctgggtc gggccggtcg gccagcccga cggcggcacg
     7141 gcgcgagccc gcatcgccga ggacggcacg atccaggtcg aggccgacgc cggcgaaccc
     7201 ctcgacgagg tggcgctgcg ctcctactgc gtcggcgccg cccaccaggg cgcgtcgtgg
     7261 gtgacgtcgg agtcgctcac ggtcgacgcc gacggccagg tgcacgacct caccatccgg
     7321 tccttcggtg tgctgcgggc cgtcgacacc ccacccatcc acgtcgacat cgcgcccggc
     7381 ggcgcgacgc cggtgccggt gtcggacgcg gtcttcgccg ccgtcgccgc agccgtttgg
     7441 gtgcaccagg gccgaccacc ggtctggccc accgggatac cgttgcggac atgaccaccc
     7501 ccgtcggccc gtacaccccc gtcgtccgca gcggcgactg gatcatctgc tccggccagg
     7561 tgggcctggc cgacgggaag ctggtcgagg gcggcgtgcg cgagcagctg acccaggcca
     7621 tggccaacgt ccggtcgctg ctcgagtccc agggcgcgtc gctcgccgac gtggtgaaga
     7681 ccacggtctt cctcgtcgac atggatgact actcggcgat gaacgacgtc tacgtgcagg
     7741 ccttcgacga gcacgccgac ggccaccgac cggcccggtc cgcggtcgcc gtcgccgccc
     7801 tccccatcgg cgcgcaggtc gagatcgagg cctgggcgag ggtgccgggg agctgaccgg
     7861 tcgtggcggg cgacgaggag tgggccgagc cggtcgagct cgtcggcggt gaggagtcca
     7921 ccgccgagat cgacagcggg cggccggtcg actggcacaa ggtggtgcgc acgggcgcgg
     7981 cggtggtggc cgcgctctcg ctgctctgga tcggccgctc gatggccgac gagcgagcgc
     8041 aggtcgcaga cgaccggtgc atgcgcgacg tcgacagcgt cctgtggcgc tacgagaacg
     8101 ccgggaaccg gatgggccgc gggttcgggg agtcaccgat cccccgcgac gtcgtcgacg
     8161 agttcatcgc ccaggcccgc gactgcggcg acgacctgct ggccgatgcc ctcgagttcg
     8221 agtacgtcac gtccttcgag gacgacgact gaacctctcg tctgtccgtc actcctggtg
     8281 tgacccgcag acgcgatttc ccggtggggt taggttgccg ggcatgcgcg ccgtgctctg
     8341 caagcagttc ggacccgtcg aggacctggt cgtcgaggag gtccccgacc ccacgcccgg
     8401 gccgggcgag gtggtcgtcg acgtggccgg ctgcggggtc aacttccccg acgtcttgat
     8461 catccaggac aagtaccagt tcaagccgga gctgcccttc tcccccggcg gcgagatcgc
     8521 cggcacggtt agcgcggtcg gcgagggcgt cgacggcctg caggtcggcg acgcggtgct
     8581 ggccacgctc ggctggggtg gcatggccga gaaggtcgcc gtctccgccg cgtcggcgat
     8641 cccggtgccg cccggcgtgg acctggtcca ggcgtcgggc ttcctgctgg cccacggaac
     8701 ctcgcaccac gccctggtcg accgcgccca cctgcagtcg ggcgagacgc tcctcgtgct
     8761 cggtgccgcc ggcggcgtcg gcctcgccgc ggtggagctc ggcgcgctgc tcggggcccg
     8821 ggtgatcgcg gccgcctcca ccgacgagaa gctcgagctg tgccgggcca agggcgccgc
     8881 cgagacgatc aactactcca ccgaggacct gcgcggccgg atcaaggagc tcaccgacgg
     8941 caagggcatc gacgtctgct acgacccggt cggcggcgac ctcagcgagc ccgcgctccg
     9001 gtcgatggcc tgggacggcc ggttcctcgt cgtcggcttc gcagctggcg acatccccaa
     9061 ggtgcccctc aacctgccgc tgctgaaggg ctgctccgtg gtcggggtgt tctggggtgc
     9121 gttcaccggc aaggagccgg agcgcaacaa ggccaacatc gccgagctgc tcggctggct
     9181 cgccgagggc aagctcgacc cccacgtgtc ggccacctac cccctcgacc aggcggtcca
     9241 ggccatcaac gacctcgccg agcgccgggc caccggcaag gtggtcgtga cgccctgacc
     9301 gagatcccgt ggatcggtca ccaccggtgg gacggatcca cggaatttcc cgaggggtgg
     9361 cggcggtctg gcacgatggc gaccatgatc ggtgccgcca tcatcgtctt cgtgctcatc
     9421 gtgttcatcc cggtgagcgt cctgatgtcg ggcagcctcg gcgcggcggc catcgggttc
     9481 ttcctgcgca acgaggccga cgtgcgccac gagggctccg agctcgtcga cctcaacggc
     9541 tgacgagcct ccgcagcgac cgcaccgcat ccggcctcgt cgccggcatc ccgctcgtgc
     9601 cgtcggctgc ggtctgcacg gcgagggtct ggtggatgtt cagccggtgg gcctcgaagg
     9661 ccacggcgga accggccatg tagaggcgcc acacccgagc ccggggcgcg ccgacgaacg
     9721 cgacggcccg gtcccagtcg gcctcgaggt tggcgaccca gcaccgaagc gtgcgggcgt
     9781 agtgctcccg cagcgactcc acgtcgcggc actcgaaccc gaggtcctgc atggtcgaga
     9841 cggtgcgtcc cacctcgtgc agctggccgt cggggaacac gtaccgcccg gggaacgact
     9901 ccggatccag cggcccctcg cccgagcacc gggagatggc gtggttgagc agccgcccgc
     9961 ccggtcggag cagggtctgg aggcgctcga agtaggtccg cacctgctcc atcccgacgt
    10021 gctcgaacat cccgatgctg gagaccgcgt cgtagggctc gtcggcgacg tcgcggtagt
    10081 cctgcaggcg gatctggacg cggtcctcga ggccggcggc gcgcacccgc tcctgggcca
    10141 gcaccgcctg ctgctcggag atcgtgatgc ccgtcgcccg cacgccgtgg tgctcggccg
    10201 cgtgcaggac catgccgccc cacccgcagc cgacgtcgag caggcgctgt cccggctcga
    10261 gccccagctt gcggcagatg tgctcgtact tgagcgcctg cgcgtcctcg agggtggtcg
    10321 atcgctcggg gaacagcgcg cacgagtagg tcatcgacgg accgagcacg agccggtaga
    10381 agtcgttgcc cacgtcgtag tggtgggaga tggcggcgcg atcccggccc ttcgagtggc
    10441 gccggccccg caggcgggcc tcctccggcg gcggctccgg ccgacgaccc aggaccccga
    10501 gccgcttcgc gtcgcgcacg aggacggcgc gctcgcgcca ggtgaggtcg aggccggcgt
    10561 cggcggtccg gtcgatcagg cggtcgcgca acccgagcag gtcgaagacc gagccgtcga
    10621 agtcgagctc gccggcgacg tgggcccggg ccaaccccag ctcacccgga tcccagagca
    10681 gccggcgcac cgcgtcgggc gagcgcacgt cgacctggag cgcggcgtcg gggtcacccc
    10741 accggctgcc gtcccagcag cgcaggccga ccggcggtgc cggtcccagg taccactcga
    10801 gcatgcgccc gatgacagcc gcagctccgt ccatctcggt ccaccccccg gagacccacg
    10861 gtcttgctgc accgagggta ccgagggaag gtgcgcgcgt gaacccccgc gctaccccat
    10921 ccagcgggac tggccgaagc ggtaccccac cgaccggacc gtctggatca acccggcgtg
    10981 ctcctcgccg agcttggccc gcaggcgccg cacgtggacg tcgacggtgc gggcaccgcc
    11041 gtagtactcg tagccccaca cccgggacag cagggtctcg cgggtgaaca ccttgcccgg
    11101 gtgggaggcg aggaacttga gcagctcgta ctccatgtag gtgaggtcga gcggcttgcc
    11161 ggagatggcg gcctggtagg tctccaggtt cagcgccagc tcgccgtact cgaccagctc
    11221 cgggcgggct ccccgaccgg tcttgtggaa caggttgcgc agacgggcgt cgagctcagc
    11281 gggctggaac ggcagcacgc agaagtcgtc gaagaggtcc tcacgcatgt ccagctcggg
    11341 cagctgctct gccgacgcga tgagcagcac cggctccagc gggatgtcac ccttgcgcaa
    11401 cgcccggcac atggcgaacg caccctcggg gttggtgacg gccaggatca ccgcgccggg
    11461 ccacccgtcg gtgggttcca tgcgctcgat gtcggcgtcg gcgccgaccg cgacccacgg
    11521 atagccggcc aggtcgaggc cctgggacag caccggcggc ggcggatccg ggtagacgag
    11581 cagcggttcc atggcc
//
LOCUS       DATISU010000290         6737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_391435, whole genome shotgun sequence.
ACCESSION   DATISU010000290 DATISU010000000
VERSION     DATISU010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6737
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391435"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VLR27_11655"
     CDS             complement(<1..205)
                     /locus_tag="VLR27_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSP04153.1"
                     /translation="MSLDRVERTFGDQTALAGLSFTVPARSITVLLGPNGAGKTTCTR
                     VITGALGPDAGAVRVFGLDPATDG"
     gene            363..872
                     /gene="ruvC"
                     /locus_tag="VLR27_11660"
     CDS             363..872
                     /gene="ruvC"
                     /locus_tag="VLR27_11660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HSP04154.1"
                     /translation="MFVWGIDPGLSRCGYAVLDTGRTGGARLVALGVFKTSPDLPVPE
                     RLHALQADVRELVADHPPGAVAIERILFSVNVRTAIGVAQAAGIVMVEAIAAGADVTE
                     YSPNEIKQAVTGDGAADKTQIETMVQRLLQIDRPIRPVDAADAAAIALCHVARAPMLA
                     RIAAAGGRR"
     gene            869..1486
                     /gene="ruvA"
                     /locus_tag="VLR27_11665"
     CDS             869..1486
                     /gene="ruvA"
                     /locus_tag="VLR27_11665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HSP04155.1"
                     /translation="MIGSLRGTLLERADDEVLVEVAGIGHRVMVSPTTAVSLGDLGDE
                     VFVWVHHHIREDTQALYGFATADARRCFTALLGAHGVGPSLALGILSVHGPGDLARVL
                     AEDDVAALCLVPGVGKKTATRLLVELKSRLDIPLDTGVPASSGSAGGAPGTGSVAAAR
                     ADVREALAGLGYGLEEIRDVLADLPDGDDPSALLKHALQRLAVSR"
     gene            1483..2517
                     /gene="ruvB"
                     /locus_tag="VLR27_11670"
     CDS             1483..2517
                     /gene="ruvB"
                     /locus_tag="VLR27_11670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HSP04156.1"
                     /translation="MREELLDPDPLEGEVVDDNGLRPRSLADFVGQARLKEHLGIILE
                     AARRRGQAVDHLLFAGPPGLGKTTLAAIVANEMGVRHVPTSGPAIERAGDLAAILTQL
                     EEGDVLFVDEIHRLPRAVEEVLYPAMEDFQLDIVIGKGPAARSIRLDLPPFTLVGATT
                     RTGAITGPLRDRFGLVERLDFYTSLELEAIVARAASILDVVLDDSGATEIARRARGTP
                     RIANRLLRRVRDYAEVRGDGTIDQATAALGLATFGVDERGLDKVDRAILSAICERFGG
                     GPVGLSTLAISVGEQTETVEDVYEPFLIQQGLLMRTPKGRVCTAGAWEHLGLVPPRSA
                     ETDGPSALFE"
     gene            complement(2530..2733)
                     /locus_tag="VLR27_11675"
     CDS             complement(2530..2733)
                     /locus_tag="VLR27_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04157.1"
                     /translation="MTRRLTIAAIAALAAAGTLGASAATADTGLTRLQADDEYILCAG
                     VRTIEVATCVEEPLGPIFDAVLP"
     gene            complement(2730..3812)
                     /locus_tag="VLR27_11680"
     CDS             complement(2730..3812)
                     /locus_tag="VLR27_11680"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSP04158.1"
                     /translation="MALRADDVDLGRDRDLVERYQNGDPSAFDELYRRYFGRLHRFCQ
                     RHTRDVHEAEEIAQEAFVKALRSMDTLTGERRFYPWMTVIAKRLTIDRHRKLARLELT
                     DEPDLGSVEPDVDHLFAAVDAGHVRDALAAVGPRHREVLLLREAEGLSYAQIGDRLEV
                     PVTTVEALLHRARKALRREYATVAGESRGMWGLPVFSLAATRLGELRAKLSERWVEIG
                     VVGAPVVAGAITAALVLTPSPGTEAVTTTAREIAPASSVDVVDEPAEPGAPVVVVGVD
                     PAVTTAPSSALAPPPAPAPPAVDAGPVDVFVGPEGVDRAADAAEEMPLRSDLGQAFAG
                     LDPRPALGTVGDGLAQALDPLLGGTP"
     gene            3942..4982
                     /gene="queA"
                     /locus_tag="VLR27_11685"
     CDS             3942..4982
                     /gene="queA"
                     /locus_tag="VLR27_11685"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HSP04159.1"
                     /translation="METASFDYDLPDSAIAQEPVEPRDAARMLVDRGAGSDPEHRTVA
                     DLPGLLGPGDVVVVNTTRVLRARLHLRKATGGAVEVLLLEPQDDGRWEALVRPGRRVP
                     PGTVLTPATDAGPPLEVEVGEILEEGRRLVRLGTDDVTAALDAHGAVPLPPYITEPLD
                     DPGRYQTVYADEPGSVAAPTAGLHLTERVLDGIRAAGADVVGVDLWVGLGTFRPISAE
                     RVEDHEMHTERYQVPEATWSACQQAERVVAIGTTTVRALESAAATGSLTGRTDLFLRP
                     GSELAVVDVLLTNYHLPRSSLLVLVEAFAGPHWRDLYVAALEAGYRFLSFGDCMLLGR
                     DGAGVEDRAVAT"
     gene            4979..6082
                     /gene="tgt"
                     /locus_tag="VLR27_11690"
     CDS             4979..6082
                     /gene="tgt"
                     /locus_tag="VLR27_11690"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HSP04160.1"
                     /translation="MTLSVEVEATAGAARATTITTARGTVTTPCFMPVGTRGVVRAVE
                     TADLEALGAQIVLGNTYHLLFRPGPDIVEQLGGIHGFSDWGGHVLTDSGGYQVFSLSP
                     KVDDDGVTFRSTYDGDEYRLTPEGAVDVQLALGADIQMVLDEVAPLPSPPEVIRRALD
                     RTADWAERARRTFLARDVPGRNQFGIVQGGTDAPMRIESAQRTVDIGFDGYAIGGLSV
                     GESREEMLPALAAAISELPPDAPRYLMGLGDPVGIVESIALGVDLFDCVLPTRFGRHG
                     TLLTSEGRLNLKNNRFATDAGPLDPDCDCRVCQRYSRAYLRHLFNVHEPTGPRLTTLH
                     NLAWTLRLTDRARAAVLDGTLDALRAEIAGVYE"
     gene            6137..6424
                     /gene="yajC"
                     /locus_tag="VLR27_11695"
     CDS             6137..6424
                     /gene="yajC"
                     /locus_tag="VLR27_11695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HSP04161.1"
                     /translation="MELLVPIAILGVMWAVLILPQQRRVKAHKAYVESLQVGDEVVTT
                     SGLFGTVTFLDADRARLRIAPDVEITMARMAIARPQSAVVPAEPPTPEDSE"
     gene            6430..>6737
                     /locus_tag="VLR27_11700"
     CDS             6430..>6737
                     /locus_tag="VLR27_11700"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04162.1"
                     /translation="MRRNGLVSLVLIVVLAIGGLGWTFAADNEPLLGLDLQGGVSVVL
                     EPTEEVAEGTLTQAIEIIRQRVDGIGVAEPEITRQDDAIVVQLPGIADREQALELVGQ
                     T"
BASE COUNT          870 a         2575 c         2414 g          878 t
ORIGIN      
        1 gcccgtcggt ggcggggtcg aggccgaaga cccgcaccgc gccggcatcc gggccgagcg
       61 cgccggtgat gacccgggtg caggtcgtct tgccggcgcc gttcgggccg agcaggaccg
      121 tgatcgaccg ggccgggacc gtgaacgaca gccccgccag cgcggtctgg tcaccgaagg
      181 tccgctcgac ccgatcgagg gacacgaccg gcgcggagcc ggcagggacc gcgtcgggcg
      241 cgcgggcaga gggggcagag gggagggtca tcaccacggg cgttgcatcg gcaccacgac
      301 ggcgacagtt gagcagagtg gggtccgcgg ccggcaccac cccgcccggt agggtcgaac
      361 ccgtgttcgt gtggggcatc gacccgggtc tctcccgctg cggctacgcc gtgctcgaca
      421 ccggtcggac cggtggcgcc cggctcgtgg ccctcggcgt gttcaagacc tcgccggacc
      481 tcccggtccc cgagcgcctc cacgccctgc aggccgacgt ccgcgagctc gtcgccgacc
      541 atccccccgg ggccgtcgcc atcgagcgca tcctgttctc ggtcaacgtc cgcaccgcga
      601 tcggcgtcgc ccaggcggcg ggcatcgtga tggtcgaggc catcgccgcg ggtgccgacg
      661 tcaccgagta ctcgccgaac gagatcaagc aggcggtcac cggcgacggc gccgccgaca
      721 agacccagat cgagaccatg gtgcaacgac tcctccagat cgaccgcccc atccgcccgg
      781 tcgacgccgc cgacgccgcc gccatcgcgc tgtgccatgt cgcccgggcg ccgatgctgg
      841 cccggatcgc cgccgccggg ggccgacggt gatcggctcg ctgcggggca ccctgctcga
      901 gcgcgccgac gacgaggtcc tggtcgaggt cgccggcatc ggccaccggg tgatggtgag
      961 cccgaccacc gcggtgtccc tcggcgacct cggtgacgag gtgttcgttt gggtgcacca
     1021 ccacatccgc gaggacaccc aggcgctgta cggcttcgcc accgccgacg cccgccggtg
     1081 cttcaccgcc ctgctcggcg cccacggggt cgggccctcg ctcgccctcg gcatcctctc
     1141 ggtgcacggc cccggcgacc tcgcccgggt cctggccgag gacgacgtcg ccgctctctg
     1201 cctggtgccc ggcgtcggca agaagaccgc cacccggctc ctggtggagc tcaagagccg
     1261 cctcgacatc cccctcgaca ccggggtccc ggcctcctcc gggtccgccg gcggcgcgcc
     1321 gggcaccggg agcgtggccg cggcccgcgc ggatgtccgc gaggccctcg ccggcctcgg
     1381 ttacgggctc gaggagatcc gcgacgtcct cgccgacctg cccgacggcg acgacccctc
     1441 cgccctgctc aagcacgcgc tgcagcgcct ggcggtgtcc cggtgaggga ggagcttctc
     1501 gatccggacc cgctcgaggg cgaggtcgtc gacgacaacg gcctgcggcc ccggtcgctg
     1561 gccgacttcg tgggccaggc ccgcctgaag gagcacctcg gcatcatcct cgaagccgcc
     1621 cgccggcgcg gccaggccgt cgaccacctg ctcttcgccg gtccacccgg tctcggcaag
     1681 accacgctgg ccgccatcgt cgccaacgag atgggcgtgc gccacgtgcc cacgtccggg
     1741 ccggccatcg agcgcgccgg cgacctcgcc gcgatcctca cccagctcga ggagggcgac
     1801 gtcctcttcg tcgacgagat ccaccgcctg ccccgcgccg tggaggaggt gctctacccg
     1861 gcgatggagg acttccagct cgacatcgtc atcggcaagg gccccgcggc gcgctcgatc
     1921 cgcctcgacc tgccgccgtt cacgctggtg ggtgccacca cccgcaccgg ggccatcacc
     1981 ggcccgctgc gcgaccgctt cggcctggtc gagcgcctcg acttctacac ctcgctcgag
     2041 ctcgaggcca tcgtcgcccg agcagcgtcc atcctcgacg tggtcctcga cgacagcggc
     2101 gccaccgaga tcgcccgccg ggcccggggc acgccccgca tcgccaaccg cctgctgcgt
     2161 cgggtgcgcg actacgccga ggtgcgtggc gacggcacca tcgaccaggc caccgcggcg
     2221 ctcgggctgg ccaccttcgg cgtggacgag cgcggcctgg acaaggtcga ccgggccatc
     2281 ctgtcggcca tctgcgagcg cttcggcggc ggccccgtcg gcctgtcgac cctggccatc
     2341 agcgtcggcg agcagaccga gacggtcgag gacgtctacg agccgttcct catccagcag
     2401 ggcctgctga tgcgcacgcc gaagggacgg gtctgcacgg ccggcgcgtg ggagcacctc
     2461 ggcctggtgc cgccccgctc cgccgagacc gacgggccgt ccgcgctgtt cgagtaggga
     2521 ccggcccggt cagggcagga cggcgtcgaa gatcgggccg agcggctcct cgacgcaggt
     2581 ggcgacctcg atggtccgca cgccggcgca gaggatgtac tcgtcgtcgg cctgcagccg
     2641 ggtgagtccg gtgtcggcgg tggcggccga ggcaccgagg gtgccggcgg cggcgagggc
     2701 ggcgatggcg gcgatggtga gacggcgggt cacggggttc ctccgaggag cgggtcgagt
     2761 gcctgggcga gaccgtcgcc gacggtgccg agggcagggc gggggtcgag gccggcgaag
     2821 gcctggccga ggtcggagcg caggggcatc tcctcggccg catcggcggc tcggtcgacc
     2881 ccctcggggc cgacgaagac atcgacggga ccggcgtcca cggccggggg cgcgggtgca
     2941 ggtggtggtg cgagggccga cgacggtgcc gtcgtgacgg ccgggtcgac gccgacgacc
     3001 acgacgggtg cgccgggctc ggccggctcg tcgacgacgt cgacgctgct ggccggcgcg
     3061 atctcgcgcg ccgtcgtcgt caccgcctcc gtgccgggcg agggggtgag cacgagcgcg
     3121 gccgtgatgg cgccggcgac caccggggcg ccgaccacgc cgatctcgac ccagcgctcg
     3181 gagagcttgg cccgcagctc gccgagccgg gttgcggcca ggctgaagac gggcaggccc
     3241 cacatgcccc gggactcccc ggcgacggtg gcgtactcgc gccgcagcgc cttgcgggcc
     3301 cggtggagca gggcctcgac ggtggtcacg gggacctcga ggcggtcgcc gatctgggcg
     3361 tagctgaggc cctcggcctc gcgcaggagc aggacctcgc ggtggcgggg gccgaccgcg
     3421 gcgagggcgt cgcgcacgtg gccggcgtcg acggcggcga agaggtggtc gacgtcgggc
     3481 tcgacggagc cgaggtcggg ctcgtcggtg agctcgagcc gggcgagctt gcggtggcgg
     3541 tcgatggtga ggcgcttcgc gatcaccgtc atccacgggt agaagcggcg ctcgccggtg
     3601 agggtgtcca tggaccgcag cgccttgacg aaggcctcct gggcgatctc ctcggcctcg
     3661 tggacgtcgc gggtgtggcg ctggcagaag cggtgcaggc ggccgaagta gcgccggtag
     3721 agctcgtcga acgcggaggg atcgccgttc tggtaccgct cgaccaggtc ccggtcgcga
     3781 ccgaggtcga cgtcgtccgc tcgtagcgcc atgttgcctc cgaacccccg agcctggacc
     3841 agctgcgcag ctgcgggtcc gctcgtcgac ccgcagcgcc tacttcgaca ccgggtcggg
     3901 ggttcccttc cgggccggcg cgacccgtag cgtcgcccga cgtggagacg gcgagcttcg
     3961 actacgacct gccggactcg gcgatcgcgc aggaaccggt cgagccgcgc gacgcggccc
     4021 ggatgctggt cgatcgcggg gccgggtccg acccggagca ccgcacggtc gccgacctgc
     4081 ccggcctgct cggcccgggc gacgtggtcg tcgtgaacac cacgcgcgtc ctgcgggcgc
     4141 gcctgcacct ccggaaggcc accggcggcg ccgtcgaggt cctgctcctc gaaccccagg
     4201 acgacggtcg gtgggaggcg ctggtccgcc ccggccggcg ggtcccgccc gggacggtcc
     4261 tcacgcccgc caccgatgcc ggaccgccgc tcgaggtcga ggtgggggag atcctcgagg
     4321 agggccggcg cctggtccgg ttgggcaccg acgacgtcac cgccgccctg gacgctcacg
     4381 gtgcggtccc gctgccgccc tacatcaccg agccgttgga cgatcccggt cgctaccaga
     4441 ccgtctacgc cgacgagccg ggatcggtgg ccgcgccgac ggccggcctg cacctcaccg
     4501 agcgggtgct cgacgggatc cgggccgccg gtgccgacgt ggtcggcgtc gacctctggg
     4561 tcgggctcgg gacgttccgg ccgatcagcg ccgaacgggt ggaggaccac gagatgcaca
     4621 cggagcgcta ccaggtgccc gaggccacct ggtcggcctg ccagcaggcc gagcgggtcg
     4681 tcgccatcgg cacgaccacc gtccgagcgc tcgagagcgc cgccgccacc gggtcgctca
     4741 ccggccggac cgacctgttc ctgcgccccg gcagcgagct ggccgtcgtc gacgtcctgc
     4801 tcaccaacta ccacctgccc cgctcgagcc tgctcgtgct cgtcgaggcc ttcgccggtc
     4861 cgcactggcg ggacctgtac gtcgccgcgc tggaggccgg ctaccggttc ctgagcttcg
     4921 gggactgcat gctcctcggc cgggacggcg cgggcgtcga ggaccgggcg gtggcgacat
     4981 gacgctctcg gtcgaggtcg aggccaccgc gggcgcggcg cgcgcgacca ccatcaccac
     5041 cgcccggggc acggtcacca ccccgtgctt catgccggtc ggcacccgcg gcgtggtgcg
     5101 ggcggtcgag accgccgatc tggaggcgct cggcgcccag atcgtgctcg gcaacacgta
     5161 ccacctgctc ttccgccccg ggcccgacat cgtcgagcag ctcggcggca tccacggctt
     5221 cagcgactgg ggcggccacg tgctgaccga cagcggcggc taccaggtgt tctcgctgtc
     5281 cccgaaggtc gacgacgacg gggtcacgtt ccggtccacc tacgacggcg acgagtaccg
     5341 cctgaccccc gagggtgccg tggacgtgca gctcgccctc ggggccgaca tccagatggt
     5401 gctcgacgag gtcgcgcccc tgcccagccc gcccgaggtg atccgccgcg cgctcgaccg
     5461 caccgctgac tgggccgagc gggcccgccg caccttcctc gcccgtgacg tgcccggccg
     5521 caaccagttc ggcatcgtcc agggcggcac cgacgccccg atgcgcatcg agagcgccca
     5581 gcggacggtg gacatcggct tcgacggcta cgccatcggc gggctctcgg tgggggagtc
     5641 gcgcgaggag atgctgccgg cgctggccgc cgccatcagc gagctcccgc ccgatgcccc
     5701 ccggtacctc atgggcctcg gcgacccggt cggcatcgtg gagtccatcg ccctcggggt
     5761 cgacctgttc gactgcgtgc tgcccacccg cttcggccgc cacggcaccc tgctcacctc
     5821 cgagggccgc ctcaacctca agaacaaccg cttcgccacc gacgccgggc ccctcgaccc
     5881 ggactgcgac tgccgggtgt gccagcgcta ctcacgcgcc tacctgcgcc acctcttcaa
     5941 cgtccacgag ccgaccggcc cccggctgac cacgctgcac aacctggcct ggaccctgcg
     6001 cctcaccgac cgggcccggg ccgcggtgct cgacgggacc ctcgacgcgc tgcgagccga
     6061 gatcgccggg gtctacgagt agcccgcacc ccgcaactag catcgtccgg tccgtccccg
     6121 accccggtgg tacctggtgg agctgctcgt cccgatcgcg atcctcggtg tcatgtgggc
     6181 cgtgctgatc ctcccgcagc agcggcgggt caaggcgcac aaggcctacg tcgagtcgct
     6241 ccaggtcggt gacgaggtgg tcaccacgtc gggcctgttc ggcaccgtca ccttcctcga
     6301 cgccgaccgc gcccgcctcc gcatcgcacc cgatgtcgag atcaccatgg cccgcatggc
     6361 catcgcccgg ccccagtcgg cggtcgtgcc ggccgaaccc cccacccccg aagactccga
     6421 gtagccccca tgcgccgcaa cggcctcgtc tccctcgtcc tcatcgtcgt gctcgccatc
     6481 ggcggcctcg gctggacctt cgccgccgac aacgaacccc tgctcgggct cgacctgcag
     6541 ggcggcgtct cggtggtcct cgaacccacc gaggaggtcg ccgagggcac cctcacccag
     6601 gccatcgaga tcatccgcca gcgcgtcgac ggcatcggcg tcgccgagcc ggagatcacc
     6661 cgccaggacg acgccatcgt cgtccagctg cccggcatcg ccgaccgcga gcaagccctg
     6721 gagctggtcg gccagac
//
LOCUS       DATISU010000291         5228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_111745, whole genome shotgun sequence.
ACCESSION   DATISU010000291 DATISU010000000
VERSION     DATISU010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111745"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..247)
                     /locus_tag="VLR27_11705"
     CDS             complement(83..247)
                     /locus_tag="VLR27_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04163.1"
                     /translation="MAAADDERSDPGTARRGPGTEDATSETSPRTEPSLVKNIERVAD
                     KVKGLLRSDG"
     gene            381..2837
                     /locus_tag="VLR27_11710"
     CDS             381..2837
                     /locus_tag="VLR27_11710"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSP04164.1"
                     /translation="MAAERSEAAALLRALPAALVLIDDEGTVVEVTDRGAAAIGRPRD
                     VLVGAPLAEHVGAAGDRLRAALAELGDDEIEVRFADPTGDGLAEVHLRVARSPFGPLC
                     DVQRRNPTTEDLLAAVLQAVASTDVGPEVLERALGQIGASNHWDVGALWVVDDAAGLL
                     RPVTTWSRDPGAARAHRDRTMGAGLAAGEGLPGRAWSEQRTVVDGDVGGDDRLADGSG
                     RFATRLHHPITAAGGQIVGVIELLATTDEDPRAWADSALRAVEPALAHLLDRVRDRMA
                     VRAAEGRLAAALDAGQFGVAGVDAGSGRVEWSSRMAALHGQTAVAGAGPLSALLAGVH
                     SDDLDEIQRALRSLPDPEATDGSPLRQVEYRVRNDEGRLRWLSTRITATVLPSGQTQV
                     AAITSDVTDRKQAEQRTQRRAMAIEGLQWVSQAIIAGRELRDTAIAVAHAATGVLGAV
                     YGVVLYQVPGDTADELAWALSGLPPGSDIPPPPVELDLPDDLAAAHGPALVDLRSQPD
                     VRAFVLGLRLPVEVGTMRSALVVPIRGEGRGRLGTMVFLHPDAGYFTEDDVRLARSIG
                     ASTGVAVENAKHHEQQRLAATAFQRELLPDSTAAVEGADVCVRYHPGRDGIEVGGDWY
                     DVIDLGDDRVGLAVGDVCGHGLTAAAHMGQFRHSFRALVQSSVSPEEALRVLNRLALE
                     ELHTTMTIVYVELDNRTGDCRTWICGHLPPLIVDRGGNEVRWLGDDGGRGPMLGFVPD
                     LDVTPSVDRLGSGELLFLYSDGLVERRGESIDEGLDRLASSFVGRSSDIETACDELYQ
                     LLADQGPAADDTALLAVRRH"
     gene            2945..3160
                     /locus_tag="VLR27_11715"
     CDS             2945..3160
                     /locus_tag="VLR27_11715"
                     /inference="COORDINATES: protein motif:HMM:NF039788.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HSP04165.1"
                     /translation="MATGTSIFLIALGAILTFAVDVTVSGLDLATVGIILMIAGVIGV
                     IVSLIWIDRATAPRTDRTVVEERTERR"
     gene            3289..>5228
                     /locus_tag="VLR27_11720"
     CDS             3289..>5228
                     /locus_tag="VLR27_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSP04166.1"
                     /translation="MARPTFRYSRWDGTQVGFDLDADAIFDEITDDLLYHGDLNAALR
                     RLMQQGFEDDRGNDVQGLRELMQRLRDARRERLENTNLGGVYDEIADALRDVVEQERS
                     ALDRRLDEARESGDARQQELAEASTTEKHMHLDMLPSDLAGQVQSLAQYDFESAEAQQ
                     RFDELMDQLRQQLAQQYFDQMAGAMSNPDPAAMQRTKDMMAALNELLEKHQRGEDTPE
                     DFQQFMEEYGDFFPEDPQDVEELLRAMAERMAQMQAMLNSMTPEQRAQLQGLAEQLLE
                     DMDLNFLASQLGQNLQQMFPQLDWDKQYDFSGDNPMGFAEAAQMMAELGDLDQLENLL
                     QSATQPGALAEADLDRARDLLGDDAARSLEQLSKLAKQLEEAGLIQNKEGRLELTPRG
                     LRRIGDNALDDLFSKLAKDKLGRHELSSSGIGHERTYETKAYEWGDPFNLHIGRTVRN
                     AVSRAGSGTPVQLTPEDFEIERTETVVRSATVLMLDLSLSMPMRDNFLPAKKVAMALH
                     ALISGQFPQDYLGLVGFSKVAHVLTPAQLPEVSWDYDYGTNMQHGLQLARQLLSRQSG
                     TKQIIMITDGEPTAHFEPGMAQPYFNYPPVQETIDATVREVIRCTKDDIRINTFMLDA
                     TQYLKTFIEKISRMNGGRAFFTT"
BASE COUNT          777 a         1951 c         1792 g          708 t
ORIGIN      
        1 gcagtcgacg ccgccgggcg caggggtcat ggcgatcgcg gccggcgcga acctactcgg
       61 gcatcgtggg gcggggcgac ggtcagccat ccgatcggag cagacccttc accttgtcgg
      121 cgacccgttc gatgttcttc accagcgagg gttcggtccg cggcgacgtc tcggacgtgg
      181 cgtcctcggt cccggggccg cgccgggccg tgcccgggtc ggatcgttcg tcgtcagctg
      241 cggccaaggt cctctccctt cgatggcgcg ccgcggcacg gcgccgtgta gcggggcctc
      301 catctccagt caccgggaac ggcagaacca gccccccagc cttggggtcg aacggggcgg
      361 gtgtgcgaac atcgaaaccc atggcggcag aacggtcgga ggcagcggcg ctgctgcgcg
      421 ccctgccggc tgccctggtc ctgatcgacg acgagggcac ggtggtcgag gtgaccgacc
      481 gcggcgcggc ggcgatcggt cgaccgcggg acgtcctggt cggcgcgccg ctcgccgagc
      541 acgtgggtgc cgccggggac cggctccgtg ccgcgctcgc cgagctcggc gacgacgaga
      601 tcgaggtccg cttcgccgac cccaccggag acggcctcgc cgaggtccac ctgcgcgtcg
      661 cccgcagccc gttcggtccg ctctgcgacg tgcagcggcg aaaccccacc accgaggacc
      721 tgctggcggc cgtgctgcag gcggtggcct ccaccgacgt cggccccgag gtgctcgagc
      781 gggccctcgg ccagatcggt gcatcgaacc actgggacgt gggcgcgctg tgggtggtcg
      841 acgacgccgc tgggctcctg cgcccggtca cgacgtggtc tcgggatccc ggcgccgccc
      901 gcgcgcaccg ggatcgcacg atgggggccg gccttgccgc cggcgagggg ctgcccggtc
      961 gggcgtggag cgaacagcgc acggtcgtcg acggcgacgt cggcggggac gaccgcctgg
     1021 ccgacgggtc cggtcggttc gcgacgcggc tccaccatcc catcaccgcc gcggggggcc
     1081 agatcgtggg ggtcatcgaa ctgctggcca cgaccgacga ggacccccgg gcctgggcgg
     1141 attcggcgct gcgcgccgtc gagcccgcgc tggcccacct gctcgatcgg gtccgcgacc
     1201 ggatggccgt gcgtgcggcg gaggggcggc tcgcagcagc cctcgacgcc ggccagttcg
     1261 gggtggccgg cgtcgacgcc gggtcgggac gggtcgagtg gtcctcccgg atggccgcgc
     1321 tgcacggcca gacggccgtg gccggtgccg gcccgctgtc ggcgctgctc gccggcgtgc
     1381 acagcgacga cctcgacgag atccagcggg cgctgcggag ccttccggac ccggaggcga
     1441 ccgacgggag ccccctgcgc caggtcgagt accgggtgcg caacgacgag ggccggctgc
     1501 ggtggctctc cacccgcatc accgcgaccg tcctgccgag cgggcagacg caggtcgccg
     1561 ccatcacctc cgacgtcacc gatcgcaagc aggccgagca gcgcacccag cgacgggcca
     1621 tggccatcga ggggttgcag tgggtcagcc aggccatcat cgccggacgg gagctgcggg
     1681 acacggcgat cgccgtggcc cacgccgcca ccggcgtgct cggtgcggtc tacggcgtcg
     1741 tcctctacca ggtgcccggc gacaccgccg acgagctggc gtgggccctc agcgggctgc
     1801 cccccggctc cgacatcccg ccgcccccgg tcgagctcga cctgccggac gacctcgccg
     1861 ccgcgcacgg gccggcgctg gtcgacctcc gcagccaacc cgacgtgcgc gcgttcgtgc
     1921 tcgggctccg gctgccggtg gaggtcggca ccatgcgctc cgccctggtc gtgccgatcc
     1981 ggggcgaggg ccgggggcgc ctcggcacga tggtgttcct gcaccccgac gccgggtact
     2041 tcaccgagga cgacgtccgc ctggcccgct ccatcggggc gtccaccggg gtggccgtgg
     2101 agaacgccaa gcaccacgag cagcagcggc tcgccgccac cgccttccag cgtgagctgc
     2161 tcccggactc gacggctgcg gtggagggtg ccgacgtctg cgtccgctac cacccgggtc
     2221 gggacggcat cgaggtcggc ggcgactggt acgacgtgat cgatctcggc gacgaccggg
     2281 tcggcctggc cgtcggcgac gtgtgcgggc acggcctcac cgctgcggcc cacatgggcc
     2341 agttccgcca ctccttccgg gcgctggtcc agtcctcggt gtcgcccgag gaggcgctgc
     2401 gggtgctgaa ccgcctcgcc ctcgaggagc tccacacgac gatgaccatc gtgtacgtgg
     2461 agctcgacaa ccgcaccggt gactgtcgga cgtggatctg cgggcacctc cccccgctca
     2521 tcgtcgaccg cggcgggaac gaggttcgtt ggctgggcga cgacgggggg cgtggtccga
     2581 tgctgggttt cgtcccagac ctcgacgtca cgccctcggt cgaccggctc ggctccgggg
     2641 agctgctgtt cctgtacagc gacggcctcg tcgagcgtcg gggcgagtcc atcgacgagg
     2701 gcctcgaccg cctcgcgagc tccttcgtcg gccggtcgtc ggacatcgag accgcgtgcg
     2761 acgagctgta ccagctgctc gccgaccagg gcccggcggc cgacgacacc gccctcctgg
     2821 ccgtgcgccg ccactgaggg tggcggcaag aaagtggcgg tggtctaggc gcttccgggg
     2881 tcgggtaccg aaccacccac acaacgggca tgcaacgggc agcccccaga aggagtgatt
     2941 ccccatggct accggcactt ccatcttcct catcgcactc ggcgcgatcc tcaccttcgc
     3001 cgtcgacgtg acggtgtccg ggctcgatct cgccaccgtc ggcatcatct tgatgatcgc
     3061 cggtgtcatc ggcgtcatcg tctccctgat ctggatcgac cgggccaccg ccccccgcac
     3121 cgaccgcacc gtcgtcgaag aacgcaccga gcgccgctga tccgacccga tcggatccac
     3181 cccggccacg ccggaaccca ctccgaacca cacgaggcag cgctcccccg cgctgcctcg
     3241 tgcgcgtctg cggcccgacc gtcccggctc gccgctaccg tgccgagcat ggctcgtccc
     3301 acgttccgct actcgcgctg ggatggcacg caggtcggct tcgacctcga cgccgatgcg
     3361 atcttcgacg agatcaccga cgacctgctg taccacggtg acctgaacgc cgccctgcgc
     3421 cggctgatgc agcagggctt cgaggacgac cggggcaacg acgtgcaggg cctgcgcgag
     3481 ctcatgcagc ggctgcgcga cgcccgccgt gagcggctcg agaacacgaa cctgggcggc
     3541 gtctacgacg agatcgccga tgccctccgg gacgtcgtcg agcaggagcg ctcggccctc
     3601 gaccgtcgcc tcgacgaggc gcgcgagtcc ggggacgccc gccagcagga gctggccgag
     3661 gcctccacca ccgagaagca catgcacctc gacatgctcc cctcggacct cgccggccag
     3721 gtccagagcc tggcccagta cgacttcgag tcagccgagg cccagcagcg cttcgacgag
     3781 ctcatggacc agctccgcca gcagctggcc cagcagtact tcgaccagat ggccggagcc
     3841 atgtccaacc ccgaccccgc cgccatgcag cgcaccaagg acatgatggc ggcgctcaac
     3901 gagctgctcg agaagcacca gcgcggcgag gacacgcccg aggacttcca gcagttcatg
     3961 gaggagtacg gggacttctt ccccgaggac ccccaggacg tcgaggagct cctgcgggcc
     4021 atggccgagc gcatggccca gatgcaggcg atgctcaact cgatgacgcc cgagcagcgg
     4081 gcccagctgc agggcctcgc cgagcagttg ctcgaggaca tggacctcaa cttcctggcc
     4141 agccagctcg gtcagaacct ccagcagatg ttcccccagc tcgactggga caagcagtac
     4201 gacttctccg gcgacaaccc gatggggttc gccgaggccg cgcagatgat ggccgagctc
     4261 ggcgacctcg accagctcga gaacctcctg cagtcggcca cccagcccgg tgccctggcc
     4321 gaggccgacc tcgaccgggc ccgcgacctg ctcggcgacg atgccgcccg ctccctcgag
     4381 cagctgtcca agctggccaa gcagctcgag gaggccgggc tcatccagaa caaggagggg
     4441 cgcctcgagc tcacgccgcg cggcctgcgc cgcatcggcg acaacgccct cgacgacctg
     4501 ttctccaagc tggccaagga caagctcggt cgccacgagc tgtcgtcctc cggcatcggt
     4561 cacgagcgca cctacgagac caaggcctac gagtggggcg acccgttcaa cctccacatc
     4621 ggccgcacgg tgcgcaacgc cgtgagccgc gccgggtccg gcacgccggt gcagctcacg
     4681 ccggaggact tcgagatcga gcgcaccgag acggtggtgc gctcggccac cgtgctcatg
     4741 ctcgacctgt cgctgtcgat gccgatgcgc gacaacttcc tgccggccaa gaaggtggcg
     4801 atggcgctgc acgcgctcat ctcgggccag ttcccccagg actacctcgg cctggtcggc
     4861 ttctcgaagg tcgcccacgt gctgacgccg gcccagctgc ccgaggtgag ctgggactac
     4921 gactacggca ccaacatgca gcacggcctg cagctcgccc gccagctgct gtcgcgccag
     4981 agcggcacga agcagatcat catgatcacc gacggcgagc ccaccgccca cttcgagccg
     5041 ggcatggccc agccgtactt caactacccg cccgtacagg agacgatcga cgccacggtg
     5101 cgcgaggtca tccgctgcac caaggacgac atccgcatca acaccttcat gctggacgcc
     5161 acccagtacc tgaagacctt catcgagaag atctcccgca tgaacggcgg gcgggccttc
     5221 ttcaccac
//
LOCUS       DATISU010000292        25434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_484853, whole genome shotgun sequence.
ACCESSION   DATISU010000292 DATISU010000000
VERSION     DATISU010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 25434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25434
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484853"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VLR27_11725"
     CDS             <1..258
                     /locus_tag="VLR27_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04167.1"
                     /translation="IVTWDTKAACTASEPASRRTRQQVEQAFGCRIDGVEEVETIAVA
                     APVHDVLDLREVAGAESPFLPTKGIVQQPTGGCRARHRAPG"
     gene            502..1785
                     /locus_tag="VLR27_11730"
     CDS             502..1785
                     /locus_tag="VLR27_11730"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HSP04168.1"
                     /translation="MHSFVRRHRDQLQVLVGVLMGVTGAAAALLADRAVELSMEIPTT
                     NALSLLAATFSALVTAGAFAFWMLPLAAQLAASSVPPTVVAERLHGRFQRLVVTVTLG
                     ALSYTAVAMASLPTSDEASAPTVTTALGAMLGVGAIAALLVAIEHAVRSTRPSVLVSE
                     AAHAVIDHIRTFGHGEAENAAADARASRSSIESVSAPATGWLHAVHEDALLRSVPEGT
                     TVSLQVGLGAFVVEGWTTVANVSGKSLPEEAGRGLESHFVVDVERAPQQALVGRLTHF
                     TDIAVHAGSGSSGAPSIVYESIWYLGAILHELVHHELGRTERRTDDGRILEWSPEPAA
                     AELVDVATDRIRQVTASEPSMALELVRVLVDVRHAAQGAGREDLVDVLGHQADLVVEQ
                     CRRADPLPADLDRVVAARSGDRRIVRDEEYATEQV"
     gene            1920..2099
                     /locus_tag="VLR27_11735"
     CDS             1920..2099
                     /locus_tag="VLR27_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HSP04169.1"
                     /translation="MADAPSNKDDAKGRVKEAAGNLTGDKDLENEGKVDKAVGGVKDV
                     ADKAADKVKDVFRKD"
     gene            2286..2801
                     /locus_tag="VLR27_11740"
     CDS             2286..2801
                     /locus_tag="VLR27_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04170.1"
                     /translation="MSDTTSDLPEGSTFDRLKAKAKQVAGDLLDRGDLAAEGTLEEAS
                     IDRADDAREKIAEAERAETEAELEARLSENEIEAERLEAEVEEQRRRDEVEQAEARTD
                     AQVEAQADQRAKAIAQQESAERSQASREQVEALSERAEAEQDVARLEAEAEAAKANAS
                     RLDALDAEKQS"
     gene            2798..3472
                     /locus_tag="VLR27_11745"
     CDS             2798..3472
                     /locus_tag="VLR27_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04171.1"
                     /translation="MTRTITRDEDQTMISQLKTLPREAVLGTLRLWRKPLGLAESVFA
                     KESADQAVSTWPPTVAYDRLASSVAHAAGVVLNDEVLLHTATVKKAKVSQLEAAARAE
                     AAAEAKRTDADQELAEKRAEAQRKEREAAQRAEKRKAQARTEEQQAKQRAKKQAAAKK
                     KAVDEAAQRTQDRIDAEAREAKKRELEAEKEALAEQREALQATKAAATTKKAAEAVKK
                     ARKSTR"
     gene            complement(3537..5690)
                     /locus_tag="VLR27_11750"
     CDS             complement(3537..5690)
                     /locus_tag="VLR27_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HSP04172.1"
                     /translation="MSIAITDDHRALSDTASDLLTKRDARGAARALLESSDEALPDLW
                     KDLVGLGWLGLHLPEAHGGSGYTLEELVIVVEQLGRVVAPGPFVPTVIASALIASAGD
                     DDLQSARLPGLADGSTIAAIGLTGSVTVSGGTASGQASPVLGGGLADVFVVRAGDDVA
                     IIDAGDGVTVDTPANLDPTRRSSRVTFDGAAATVVPGAARLFVDLARVILSAEAAGLA
                     QETTDMAASYAKEREQFGRPIAMYQAVKHHCANMAVARELTTSAVWDAARAAATGGDQ
                     LSYAAAVAATLAGPAADLCANLNTQVHGGIAITWEHDAHLYMRRATTLLTLLEPKQAA
                     ADLVDLVRSGVTRAKAVELPPEAEAIRDEVRTFAESIKDLSKEEQRAKLIETGYVMPQ
                     WPKPYGRAAGAIEQLVIEEEFAKAGIQRPAYGITAWNILTIIQYGTQDQLDRWVLPAL
                     NQDVIWCQLFSEPDAGSDAAGVKTKATRVDGGWLVNGQKVWTSGAHVAGMGFATVRTN
                     PDVPTHDGITMMAIDMHAEGVEVRPLKMTNGGSEFNEVFFNDVFVPDDDVVGPVDGGW
                     TVARSTLGNESVSIGGGGGGMSMPGAGLVPPLDANPDRLSGGAARVGRYIAQHQAMGL
                     LNLRAANRAVAGGEPGPEGAMTKLVLSEVGHEAAAILTELNGPEAAFMDGAGGMSNQL
                     VLMHRGMSIAGGTSEIKRNQIGERILGLPRDPLLK"
     gene            5834..6559
                     /locus_tag="VLR27_11755"
     CDS             5834..6559
                     /locus_tag="VLR27_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP04173.1"
                     /translation="MEPRPLDLAADPDPSISRVLVVEDDRNLADVVARYLERDGFEVE
                     VVHDGDAGLRRALESLPDLVVLDLMLPGLHGIEVFRRLRQVAPIPVVMLTARGSEEDK
                     VTGLELGADDYVAKPFSPRELTARVRAVLRRASASVEVRGSSVLRAGALELDLVAHEA
                     RRDGEPLPLTARELDLLAYLMAHPRRAFTRRELLREVWGHEIGDTATVTVHVQRLRDK
                     AEADPRQPQHLRTVRGIGYRFEP"
     gene            6556..7782
                     /locus_tag="VLR27_11760"
     CDS             6556..7782
                     /locus_tag="VLR27_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSP04174.1"
                     /translation="MSRPRGGARVAWVGIAGAGVVAALGAAGAAGLGVRDTLVLLALA
                     GGAATAAAVGTVALLTRMSRRDLRVQLVVVALVAVAATAAGVAVAAWAMFLSGHDLGV
                     LGVVLLLSASVGVSAAWILGWQVGDGLDGLGQRIDALAGDDVPDPPRRLPTAELQRLS
                     EVLTVTHRQLVDARQRAARLEASRRELVAWVSHDLRSPIGSVRAMAEALEDGLVAEPA
                     DVASYHRAIVQEVQRLAALVDDLFELSRIEAGAPPLDVPFVPLHELVADVIEAARVRA
                     DAQGVALHAEVHDLGPELVVAADVRRAIDNVVDNAIRHTPAGGSVTATARADGTGLVV
                     VVWDECGGIPASDIDRIFDVAFRGDDSRSSDTGGGGLGLAIARGLVEARAGGISVRNR
                     EPGCEFTVRLPAEVPS"
     gene            7779..9362
                     /locus_tag="VLR27_11765"
     CDS             7779..9362
                     /locus_tag="VLR27_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSP04175.1"
                     /translation="MTGRADDASRTRRERRWRAAVAGVIAAGGALAAGEVPPALAGEA
                     EGPVTAVGNEFVDRFAASLKELAVALFGQNDKVALIVGIVVISLLLGAALGLLALRSF
                     VLAALGFATFAAIGLAAQVADTGAGVLVPALSALAGAAAGAGVLALLVAQIGRAPDLA
                     TTLRRAGDVEDPRVRTPDRRTFLAVGAAAGAAAALTGAGTWSLRGRSAVDSQRAALVL
                     PRPVRATPVRASGLEIPGLSPYVTPTDDFYRIDTALVTPRVDADSWRLRVSGLVEEPF
                     ELTFDELLDMDMVEEPVTIACVSNEVGGDLVGNAVWLGVPLTALLDRAGVRPEGTQVV
                     GRSTDGFTAGFPTDVATDGRVAMVAVGMNGEPLPTDHGYPARLIVAGLYGYVSATKWL
                     TEIELTRFEDFDAYWIPRGWSKLGPVRTQSRVDVPRGTVDAGTVPIAGVAWAPDIGIA
                     AVEVRIDDGPWEPAELHGTANDSTWAQWVHQWDATPGAHTIRCRATDATGSTQTAERS
                     DPAPDGATGLHTVQVTVRG"
     gene            9402..9662
                     /locus_tag="VLR27_11770"
     CDS             9402..9662
                     /locus_tag="VLR27_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04176.1"
                     /translation="MHRLSRSTRLLLGLAVGIGLTAGACGDDDDTVETDPTQEVDEMT
                     EEEMTEDEMTEDEMTEEEMTEDEMEDEMTEDEMMEEDMEDGG"
     gene            9707..10771
                     /locus_tag="VLR27_11775"
     CDS             9707..10771
                     /locus_tag="VLR27_11775"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HSP04177.1"
                     /translation="MNRVLPPDERLNLRTSMPFLAFHLIPVAAIWTGVPVEAVVLAVV
                     SYVARMFFITAGYHRYFAHRAYKLPRWAQAVMAFGGTTALQKGPLWWAAHHRDHHRWS
                     DTDQDVHSPIRGFWWSHVGWILCDKYSATDESRIRDFAKYPELRWLNRNDWVGPVSLA
                     VVSFLVAGWAGLVIGFGASTIAVWHGTFLVNSMAHVVGRRRYVTSDSSRNSAVIAVLT
                     LGEGWHNNHHHYQASARQGFRWWEWDPSFYVLWALERVGIVRDLKRPSRQIMQADLVE
                     AGHFDIGMFRTGWQRAAARLVAVGDHAGEALAERRQALEDRIEAVRHDLDAHRRAQRD
                     AMAELAAVRRAARTGRPLGA"
     gene            10855..11577
                     /locus_tag="VLR27_11780"
     CDS             10855..11577
                     /locus_tag="VLR27_11780"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSP04178.1"
                     /translation="MAARTARTNRRRTRTVLPIVVALAAAVVAIVMAVGAGGASDDTT
                     RDVAADAPRAGDAPLTGPEIRGAGPMAKGADDAPVVMVMYSEFQCPFCGKFARDTEPE
                     LVERFVEDGTLRIEWRDLPYLGPESLQAAVAGRAAAEQGAFWELADVLFADQAPPNSG
                     RLTAEHLVEVASELGLDAERFRADLADEDLVEAVRADVDEAVGFGVTGTPAFFVNGQV
                     IMGAQPVEVFVEAIEQAAAAAG"
     gene            11584..12435
                     /locus_tag="VLR27_11785"
     CDS             11584..12435
                     /locus_tag="VLR27_11785"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HSP04179.1"
                     /translation="MSEVTLLAAFAAGTLALLSPCSALLVPSFFAYAFGTRTALLART
                     ALFALGLAAVLVPLGMGSSAASSLVYGNRQTMIAVAGWAIIALGVLQLLGKGFTLPFV
                     GRLQARTAGIDARAGALPTIALGAVYGLAGFCSGPVLGAILTVAATEEAPARGGMLLA
                     VYALGMAAPLFVLAALWDRFDLGRRQWLRGRPVHLGPVRTHTTSLLSGTLFVVIGALF
                     LSSEGMVGLTGVFGDTTDLEFDAQAWISDRLAVVPDWSVPALIAVVAAGVALRRARDE
                     APEQVEG"
     gene            complement(12448..12618)
                     /locus_tag="VLR27_11790"
     CDS             complement(12448..12618)
                     /locus_tag="VLR27_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04180.1"
                     /translation="MTTTETPPTPGDAPTEDEAARPGDEPGAVETVATAATGMVLGGA
                     ALWFLAPICFVC"
     gene            complement(12631..13125)
                     /locus_tag="VLR27_11795"
     CDS             complement(12631..13125)
                     /locus_tag="VLR27_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04181.1"
                     /translation="MTLADRWGTVLAWPTRRWAVAAAATAAYVLVVGIPTDLVPNPFF
                     GREIPPTWWSWPSLVVSAVLAGLLAATYVGTGDPADTVDPDARRGMAGGVLTFFAVGC
                     PVCNKLVLIALGSAGAITWFEPVQPLLQLAAMGLLLWALDTRLGNQLACPVPATAGAG
                     GSDT"
     gene            complement(13122..13811)
                     /locus_tag="VLR27_11800"
     CDS             complement(13122..13811)
                     /locus_tag="VLR27_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSP04182.1"
                     /translation="MEDTVDDAQHRSELFDRLAVVGKAFASANRLKIIDLLGQGERSV
                     DSIAREVGLGVTTASAHLQILKLANVVATRTEGTRVFYRLAGDDVATLYASLGAVART
                     HSADVERALATYFGTGALDDVELITRKELRRRMKAGDVVVVDVRPPEEFEAAHIPGAR
                     SVPFGDLLEHLAEVDDDVEIVAYCRGAHCVLAHDAVRLLTAEGRSAVRLEDGMLEWRA
                     AGLPVAAGAGR"
     gene            13889..14323
                     /locus_tag="VLR27_11805"
     CDS             13889..14323
                     /locus_tag="VLR27_11805"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HSP04183.1"
                     /translation="MGDPGTRYDLSPAGIDAMLEAEFPGTGNSCTELGPTHAVARRPV
                     DSAAIRPGGFVSGPTQFGLADAALWYLVFGAIGRIEPMALTSELSIRFLRPAQGSVLW
                     ARADLASAGSRSIVGSVTVWCDDRDDKPSSVAQGTYVLPRAG"
     gene            complement(14326..16029)
                     /locus_tag="VLR27_11810"
     CDS             complement(14326..16029)
                     /locus_tag="VLR27_11810"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HSP04184.1"
                     /translation="MKSIGFILSYLATSQARRSAKILGWMLALLAVIVAVYSALFHEI
                     MEHEGQRYSWITAIYWTLVTMSTLGFGDITFESDLGRTFSVVVLLSGSVFILVLLPFT
                     FIQFVFLPWMESTQRARAPRRVDDDVSDHVILTSTGPIEAALRTRLDRAGIAHVTLVA
                     DLDEALRHHDEGLTVMVGELDDPATYAAAGVERASLVATTQADTTNTNVAFTVREISE
                     SIRIVATANAEASIDILELAGCDEVLRLGEMLGAALARRVLAPDGRSQVVGRFDELLI
                     AEAAAPDSLIGKPLLGSGIRDDTGLTVGGVWDRGELLIARPSTVLTETSILVLAGSIE
                     QLARYDERFAADDQPDRSADPPVVIIGGGRVGRAVSRHLRAMEIPNRIVEKQSDRIRD
                     GADYVEGDAADIAVLKRAGIDDAPSVVVTTHDDDVNVYLTNYCRRLRADAQIIARSRL
                     DRNVSTLHRAGADFVLSYASTGANAIWNMLSADNTLQLAEGLDVFRVPVPRKLAGKPL
                     TQARIREETGCTVVARVSDGQFEPNPDPTVPLPAEGELVLVGDSESEHRFLSRYRTSP
                     L"
     gene            complement(16071..17501)
                     /locus_tag="VLR27_11815"
     CDS             complement(16071..17501)
                     /locus_tag="VLR27_11815"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HSP04185.1"
                     /translation="MGRRLAIAVVFATSAAILIVEITAGRLLAPYVGVSLETFTAIIG
                     VILAGIATGAGVGGWLADRHDPRRLLGPSLFVGGALVWIAVPIVRAIGPEVDGNPLDI
                     VVLATMAFFLPAAVLSASTPIVAKLRLSTLEETGSVFGGLSAAGTFGGLAGTFLTGFV
                     LVALLGTRTIMFVVGLLLVVGGVLLEWALRRSLPAASTAVVFVVSGLAVYGFPPECDV
                     ETRYACASVVVDEDDPSRRSLYLDTLHHARVDVDDPTHLDLRYIRLFADVAEALPPGP
                     IDALHIGGGGFTVPTYLAAERPGSSSHVIEIDPGVVDIAEDRLGLETGPDLTVAVSDA
                     RTEIPRLADDAYDLVVGDAFSGLSVPWHLTTVELAGKVDRVLRDGGIYVLNVIDGGDS
                     AFARAELATLLEVFDHVQVILPGGEPPDTPRNQVVVASDQPIADLDIDPADGVLRDEA
                     FVRSYIDGAEVLTDDHAPVDQLLRGS"
     gene            17558..18634
                     /locus_tag="VLR27_11820"
     CDS             17558..18634
                     /locus_tag="VLR27_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255838058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:17903..17905,aa:Sec)
                     /transl_table=11
                     /product="ResA-like WAxxUGC motif-containing protein"
                     /protein_id="HSP04186.1"
                     /translation="MLLTSTTTDPAAFAAATGWAVKPEGACRADICVPLPDEVRVDGG
                     LDVTVLADRLGMPVLRDDDHGLVALGPASVVGRALETAEAPELELPDADGNPFRLSSL
                     HGKKVLLVAWASWUGCRDDLPVWQALRTELAPKGVEVVTVALDTGGVAAAGPWIAKAS
                     PEHPSLIDEAHVSAERFGFVNVPNAVWIDEDGMVVRPSHPAHVKVSKLREIEVTPDMG
                     YLGEMLTELQKFNVDATTYLDAMRDWVEHGADSRWALSPDQVVDASRPRPREHAEAAA
                     AFELGQHLHRAGHPDDAVAWFKRAHELHPENWTYKRQAWTFVTTPEGEASDLIQADND
                     VYGTSWLDEVRRLGPETYYEPFDA"
     gene            complement(18613..19626)
                     /locus_tag="VLR27_11825"
     CDS             complement(18613..19626)
                     /locus_tag="VLR27_11825"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HSP04187.1"
                     /translation="MATTGIPVIDCAEALAGGDATVAAAAIDAACREHGFFVVTGHGI
                     EPELLAALDRAARDFFARPAAEKERIAMARAGAAWRGWFPPGGELTGGVPDGKEGIYF
                     GREDGPDHPRVQAATPLHGANLFPDEPAELAPLVLEVLDRLTDLGQGVLELVALGLGL
                     ERDWFRRHLTADPTILFRIFHYPPAGEVPDASWGVQEHTDYGLLTLLAQDDVGGLEVR
                     TPGGWVEAPPVPDSFVCNLGDMLERVTGGAYRSTPHRVRRPSRGRLSFPFFLDPSWDA
                     RIVPVPGFGDTGSPPPPRWDGRSVFDWDGTYGGYLSDKVARVFPGQAGLVQPSDAQAS
                     KGS"
     gene            19690..20352
                     /locus_tag="VLR27_11830"
     CDS             19690..20352
                     /locus_tag="VLR27_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP04188.1"
                     /translation="MESSPGTSELRAIEAEVRAHPHGRVPRELRRRQLLAVAEQLFVD
                     RGYAATSMDDVAAGAGVSKPIVYELVGSKHEVFEACMARAADALAARVADAVLHAGEG
                     DATRLRAGALAWFEFIGERRSLWDALLASDDAPASAAIEAVRARQDGFVAAQLAATAQ
                     AGGAAVDPELVEAVATAMNGAFEALGRWWSRHPDRSATELADLYTALVLPGLEGLLGW
                     SP"
     gene            20411..21451
                     /locus_tag="VLR27_11835"
     CDS             20411..21451
                     /locus_tag="VLR27_11835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HSP04189.1"
                     /translation="MKLSMTLNYSGDPVQAAATAADYEKAGVDMIWVAELYSFDAVSI
                     LGYLAAKTETMELASGLFPIYSRTPALTASTAAGLDAVSQGRFVLGLGASGPQVIEGW
                     HGVKYDKPLTRTRELIDICRIVWRRERLEYQGDAYTLPLPADQGSGLGKPLKLINRPV
                     RENIPIWVASLGHKNVSMTAELADGWLPVFFHPDKARDVWGDDLDDGFAKRSSDLGPL
                     QIAAGGTVAITDDKSVADGIRDFARPMTALYVGGMGAKGKNFYNNIFKRYGYEEEAEK
                     IQDLYLDGKKDEAAALVPDDFLDATAMVGDEGWVKERIQAYKAAGVTHLQITPVGDKP
                     LEIIEKVKAWAE"
     gene            complement(21420..23072)
                     /locus_tag="VLR27_11840"
     CDS             complement(21420..23072)
                     /locus_tag="VLR27_11840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSP04190.1"
                     /translation="MLELVRRMPHLVGMTDGSGEVLWLNEAGRRFVGVRPGQRLSTAD
                     LFTDVVFDRYYASIRPALVRDGVWTGELPVRRADGTIGVVDVVLTGDVDSDGEVRWIG
                     ALAVDVTSQHEREAALSHQASHDPLTGLANRVLLLDRLDVAVSIATRLGSPVAVLTLD
                     LDGFKAVNDEHGHAVGDRLLQQVTARIQSAVRPADTVARLGGDEFVVIVHPPEEQSAA
                     MAVAERIRDQIGMLDYVIGEHRIRVTASIGLTVSNPGVHAEPDALLAAADRGMYRAKR
                     SGGNCVRVAGNDHSGILGHAEDMGDQLARALDEGQVVAGFEPVIDLRSQELCALQALA
                     RWDHPDLGRLPARSFAEEALVTGYADLIWWAATQYALRAAAAAQSTVPIHITLATSQL
                     RDEDVVGRLRALRELAPDAEVHLRVDAHSVLDITAMGQEVIDVLGRDGLRLVLSGHGR
                     RGLPLGLLASLPLAAVELDPTLVRDAGENPKAIALATRLATTLDVPCIAQVDDDAELP
                     RLAVLGVTSASGWVTGCEWDADRLAAEMAARRVTPPRPSPSR"
     gene            23243..23944
                     /locus_tag="VLR27_11845"
     CDS             23243..23944
                     /locus_tag="VLR27_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04191.1"
                     /translation="MDEATNGPGDGSGAPAGPYERLGVLARQQVEVADGFRHVELFTM
                     QGLLTVLWHGPVEADRAVIMLGGAMGGLLGPADGLYHDLGVALADQGIATFRVSYRKA
                     NDFGRCVHDALAIADVAARQGARRFVTVGHSFGGAVAVQLGMALGDHAGGVVTLATQS
                     AGCEDADGLADTPMLLVHGDRDEILPAFASEVVHGLAGGHGELMILPGAGHLLTEAGD
                     ELRAKLLDWIPTHLA"
     gene            complement(23984..25189)
                     /locus_tag="VLR27_11850"
     CDS             complement(23984..25189)
                     /locus_tag="VLR27_11850"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP04192.1"
                     /translation="MGAHLRIDPKLPAPFQRLRAAAWASNLGDGVRNAALPLIAAGID
                     GSATTVAVVAAAGTLPFAVLGLPAGTMADRRRRVPLIVSAHLFRFVVMAGLAAVILLD
                     ATTVAVLVGGAFLLGCGEALADSSSPALLPDLVDDDRLEEANGELETAELVANDLVGP
                     SIGGTLTALSSSAPFVVDAASFLVAGALVRTVDVDESHTHDPPASTWWADLREGARIS
                     WTNPVLRSTGALIVLLQLGSIAAIAPIVIYLTERLDLSATGYGIFLSIGSLGGLAGAR
                     SVKRLVKRHRPFHVLLGALAATVVSFLLMAVPVLPVVAVGFALSFAAVVTGRIVVIAA
                     RQRSVPGRLLGRAQGAIRSLLWTAATVGALLGGAISDGLGMQAPFLFAAAVTLLASLL
                     LAPSLRRVL"
BASE COUNT         3569 a         9020 c         9455 g         3390 t
ORIGIN      
        1 atcgtcacct gggacaccaa ggccgcgtgc acggccagcg agcccgcgag ccgcaggaca
       61 cgccaacagg tcgagcaggc cttcggatgc cggatcgacg gcgtggagga ggtggagacc
      121 atcgccgtcg ctgcaccggt ccacgacgta ctcgaccttc gcgaggttgc gggcgcggag
      181 tcgccgttcc ttccgacgaa gggcatcgtc cagcagccga ctggaggctg ccgtgcgcgg
      241 caccgggcac ccggttgatg tggacgaggg cggccctggc gttcttgtga ccatccgcgc
      301 cgtgggcgcc ggtgacatcg atgtggccgg cgagggaggc aacgacctcc tcgactcccg
      361 cctcgaccac cgacgactcc atggatcaaa aaggtacgaa gggggtgctg acgagtgccc
      421 ttctccgcga ttggcgcgct gaccaccgtc ggcgcacccg accccatggt gaggctcgga
      481 cggatcgggt agtgcccgaa catgcactcg ttcgtgcgtc ggcaccggga ccagctccag
      541 gtgctggtgg gcgtcctcat gggggtcacg ggcgcggcag cggcgctcct cgccgatcgt
      601 gccgtcgagc tctcgatgga gatccccacg acgaacgccc tgtcgctgct cgccgcgacg
      661 ttctctgcgc tcgtgaccgc cggtgcgttc gccttctgga tgctgccgct ggccgcccag
      721 ctcgcggcgt cgagcgtccc acccaccgtc gtcgccgagc gcctgcacgg ccggttccag
      781 cgactggtcg tcacggtgac gctcggggcc ctctcgtaca cggccgtcgc gatggcgtcg
      841 ctgcccacgt ccgacgaggc atccgccccg acggtcacca ctgcgctcgg ggccatgctc
      901 ggcgtgggag ccatcgctgc gctgctcgtg gcgatcgagc acgccgtgcg ctcgacacgg
      961 ccgagcgtcc tcgtgtcgga ggctgcccac gccgtcatcg accacatccg gacgttcgga
     1021 cacggcgagg ccgagaacgc ggccgctgac gctcgcgcat cccggtcctc gatcgagtcc
     1081 gtctcggcgc cggcgaccgg gtggctgcac gcagtccacg aggatgccct gctccgttcc
     1141 gtgcccgagg gcacgacggt gtcgctccag gtgggactcg gcgcgttcgt cgtcgaaggg
     1201 tggaccacgg tcgccaacgt cagcggcaag agccttccgg aggaggccgg ccgagggctc
     1261 gagagccact tcgtcgtgga cgtggagcga gcgccccagc aggcgctcgt cgggcgactc
     1321 acgcacttca ccgacatcgc cgtgcacgcc ggatccggga gctcgggggc tccctcgatc
     1381 gtctacgagt cgatctggta cctgggtgcg atcctgcacg agctggtgca ccacgagctc
     1441 gggcggaccg aacgtcgcac cgatgacggg cgcatcctcg agtggtcgcc ggagccggcc
     1501 gccgccgagc tcgtcgacgt ggcgaccgat cgcatccggc aggtcactgc gtcagagccc
     1561 tcgatggcgc tcgagctcgt gcgcgtgctg gtcgatgtcc gccacgccgc acagggtgcc
     1621 ggccgggagg atctggtcga cgtgctcggc catcaggccg acctggtcgt cgagcagtgc
     1681 cgtcgtgcag atccccttcc ggcagacctc gatcgcgtgg ttgcggcgag gtccggcgat
     1741 cgacggatcg tgcgagacga ggagtacgca accgagcagg tctgaccggc atcgttcgac
     1801 cgtcaccgaa gggtggcggc aaccgctcgc tgcggtgctg acgtcggcgc gtctcgtcgg
     1861 tgcccgtacc gggaagagat ccgccggttc acctcacccg aacgaaaagg atcaccacca
     1921 tggctgacgc acccagcaac aaggacgacg cgaagggccg cgtcaaggaa gcggctggca
     1981 acctgaccgg cgacaaggac ctcgagaacg agggcaaggt cgacaaggca gtcggcggcg
     2041 tcaaggacgt cgcggacaag gctgcggaca aggtcaagga cgtgttccgc aaggactgag
     2101 aagaccccga tcgaggtctt cgcaccacac ggccccgggt cccgcccggg gccgtggcgc
     2161 gcccgggacc gtgttcgtgg acctgtgacg catgtcttcg ccctcgggtg acgagacctg
     2221 ttgcgaacgg gtaacgacgt acccgcgggt aacagcatcc cccgaccatc caggagcagg
     2281 cacccatgtc cgacaccacg agcgatctcc ccgaaggcag caccttcgac cgactgaagg
     2341 ccaaggccaa gcaggttgcc ggcgatctcc tggatcgcgg cgacctcgcc gccgaaggca
     2401 ccctcgaaga ggccagcatc gatcgggctg acgacgcccg cgagaagatc gccgaggccg
     2461 agcgcgccga gaccgaagcc gagctcgaag cccggctgtc cgagaacgag atcgaggccg
     2521 aacgtctcga ggccgaggtc gaggagcagc gtcgccgcga cgaggtcgag caggcagagg
     2581 cgcggacgga cgcccaggtc gaggcccagg ccgatcagcg tgcgaaggcg atcgcccagc
     2641 aggagtccgc cgagcgcagc caggcatccc gcgagcaggt cgaggcactc agcgagcgcg
     2701 ccgaggccga gcaggacgtc gcccgcctcg aggccgaggc cgaagcggcc aaggccaacg
     2761 cctcccgact cgacgccctc gacgccgaga agcagtcgtg acccgcacca tcacccgaga
     2821 cgaggaccag accatgatct cccagctcaa gaccctgccc cgcgaagccg tgctcggcac
     2881 cctgcggctg tggcgcaagc ccctcgggct cgccgagtcc gtgttcgcca aggagtcggc
     2941 cgaccaggcc gtctccacct ggccccccac ggtcgcctac gaccggctcg cctcctccgt
     3001 ggcccacgcc gcgggcgtcg tcttgaacga cgaggtcctg ctgcacaccg ccacggtgaa
     3061 gaaggccaag gtcagccagc tcgaggccgc tgcccgagcc gaggccgcgg ccgaggccaa
     3121 gcggaccgac gccgaccagg agctcgccga gaagcgggcg gaggcgcagc gcaaggagcg
     3181 cgaggcggcg cagcgagccg agaagcgcaa ggcgcaggcc cgcaccgagg agcagcaggc
     3241 caagcagcgt gcgaagaagc aggccgcggc caagaagaag gcggtcgacg aggctgccca
     3301 gcgcacgcag gaccgcatcg acgccgaggc ccgcgaggcc aagaagcgcg agctcgaggc
     3361 cgagaaggaa gcgctcgccg agcagcgcga ggcgctccag gccaccaagg ctgcagccac
     3421 caccaagaag gcggccgaag cggtcaagaa ggcgcgcaag tccaccaggt gatctgacgc
     3481 cgcagcgcga gacgaggggc ccgaccgggg cccctcgctc cgcgtccgga gggctgctac
     3541 ttgagcagcg ggtctcgggg caggccgagg atccgctcgc cgatctggtt gcgcttgatc
     3601 tccgacgtcc cgccggcgat ggacatgccg cggtgcatca gcacgagctg gttgctcatg
     3661 ccgccggcac cgtccatgaa cgcggcctcg gggccgttca gctcggtgag gatcgcggcg
     3721 gcctcgtgtc cgacctcgga caggaccagc ttggtcatgg cgccctcggg gccgggctcg
     3781 cccccggcga cggcccggtt ggccgcacgc aggttgagca ggcccatggc ctggtgctgc
     3841 gcgatgtagc gcccgacgcg ggcggcgccg ccggagaggc gatcggggtt ggcgtcgagc
     3901 ggcggcacga gcccggcacc cggcatcgac atgcccccgc caccgccgcc gatgctcacg
     3961 ctctcgttgc cgagggtgga gcgggccacg gtccagccgc cgtcgaccgg gccgacgacg
     4021 tcgtcgtcgg ggacgaagac gtcgttgaag aagacctcgt tgaactccga cccgccgttc
     4081 gtcatcttca gcggccggac ctcgacgccc tcggcgtgca tgtcgatggc catcatcgtg
     4141 atgccgtcgt gcgtggggac gtcggggttg gtgcgcacgg tggcgaagcc catgccggcg
     4201 acgtgggcgc cggaggtcca gaccttctgg ccgttgacga gccagccgcc gtcgacgcgg
     4261 gtggccttgg tcttgacgcc ggcggcgtcg gagccggcgt cgggctcgga gaacagctgg
     4321 caccagatga cgtcctggtt cagcgcgggg aggacccagc ggtcgagctg gtcctgggtg
     4381 ccgtactgga tgatggtgag gatgttccag gcggtgatgc cgtaggcggg acgctggatg
     4441 cccgccttgg cgaactcctc ctcgatgacc agctgctcga tggcgccggc ggcccgcccg
     4501 tagggcttgg gccactgggg catcacgtag ccggtctcga tcagcttcgc ccgctgctcc
     4561 tccttcgaga ggtccttgat cgactcggcg aaggtgcgca cctcgtcgcg gatggcctcg
     4621 gcctcggggg gcagctcgac ggccttggcc cgggtgacgc cggagcggac caggtcgacg
     4681 aggtcggcgg cggcctgctt gggctcgagc agggtgagca gggtggtggc ccggcgcatg
     4741 tacaggtggg cgtcgtgctc ccaggtgatg gcgatgccgc cgtggacctg ggtgttgagg
     4801 ttggcgcaca ggtcggcggc ggggccggcg agggtggcgg cgacggcggc ggcgtagctg
     4861 agctggtcgc cgccggtggc ggcggcgcgg gcggcgtccc acacggcgct ggtggtgagc
     4921 tcccgggcga cggccatgtt ggcgcagtgg tgcttcaccg cctggtacat ggcgatgggc
     4981 cggccgaact gctcgcgctc cttcgcgtag gaggccgcca tgtcggtggt ctcctgggcg
     5041 aggccggcgg cctcggcgga caggatgacg cgggcgaggt ccacgaacag gcgagcggcg
     5101 ccggggacca cggtggcggc cgcaccgtcg aaggtgacgc gcgacgagcg gcgggtggga
     5161 tcgaggttgg cgggggtgtc gaccgtgacg ccgtcgccgg cgtcgatgat ggcgacgtcg
     5221 tcgccggccc gcacgacgaa cacgtcggcg aggccgccgc cgagcacggg gctggcctgg
     5281 ccggaggcgg tgccgcccga cacggtgacc gagccggtga ggccgatcgc ggcgatggtg
     5341 gagccgtcgg cgaggccggg caggcgtgcc gactggaggt cgtcgtcgcc ggccgaggcg
     5401 atgagcgcgc tggcgatgac ggtgggcacg aagggtccgg gggccacgac ccggccgagc
     5461 tgctcgacga cgatgaccag ctcctcgagg gtgtagccgg agccgccgtg ggcctcgggg
     5521 aggtggaggc cgagccagcc gaggccgacc aggtccttcc acaggtcggg gagcgcctcg
     5581 tcgctggact cgagcagggc tcgagcggcg ccgcgggcgt ctcgcttggt caggaggtcg
     5641 gacgccgtgt cggacagggc gcggtggtca tcggtgatcg cgatcgacat ggcaggagac
     5701 taggcaacgc gggttgccag gtgcccgtcg gtggccctcc cgtccgctct cgtccgcatc
     5761 atccgcataa cggttggcga aacaagcggg cggcgcccgg gacacggcgg ccctgcgagc
     5821 gtacgttccc cccatggagc cccgaccgct cgacctcgct gcggatccgg atccgtcgat
     5881 cagccgggtg ctggtggtgg aggacgaccg gaacctcgcc gacgtggtgg cgcggtacct
     5941 cgagcgcgac gggttcgagg tggaggtcgt ccacgacggc gacgccggcc tgcgccgagc
     6001 cctcgagtcc ctgcccgacc tggtggtgct cgacctcatg ctccccggcc tgcacgggat
     6061 cgaggtgttc cgccggctgc ggcaggtcgc cccgatcccg gtcgtcatgc tcacggcgcg
     6121 gggctcggag gaggacaagg tcaccgggct cgagctgggc gccgacgact acgtggccaa
     6181 gccgttctcg ccccgggagc tcacggcgcg ggtgcgggcc gtgctccggc gcgcctcggc
     6241 ctcggtcgag gtccggggca gctcggtgct gcgcgccggt gcgctcgagc tcgacctggt
     6301 cgcccacgaa gcgcggcggg acggcgaacc cctgccgctc accgcccggg agctcgacct
     6361 gctcgcgtat ctgatggccc acccccgtcg tgccttcacg cgacgggagc tgctccgtga
     6421 ggtgtgggga cacgagatcg gtgacacggc gacggtcacc gtccacgtcc agcgcctgcg
     6481 cgacaaggcc gaggccgacc ctcggcaacc ccagcacctg cgcaccgtgc ggggcatcgg
     6541 ctaccggttc gagccatgag ccggccccgg ggaggcgcgc gcgtcgcgtg ggtgggcatc
     6601 gccggagcgg gggtcgtggc cgcgctcggt gcggccggcg ccgccggtct cggcgtgcgg
     6661 gacaccctcg tgctcctcgc cctggccggg ggagcggcca cggcagctgc ggtcgggacg
     6721 gtcgccctgc tcacccgcat gagccgtcgc gacctgcgcg tgcagctggt ggtcgtggcg
     6781 ctcgtggccg tcgcggccac ggcagccggg gtcgcggtcg ccgcgtgggc gatgttcctc
     6841 tccgggcacg acctgggcgt gctcggcgtc gtgctcctgc tgtcggcgtc ggtgggggtg
     6901 agcgccgcct ggatcctcgg ttggcaggtc ggcgacggac tggacgggct ggggcagcgc
     6961 attgacgcgc tcgccggtga cgacgtcccc gacccgccgc gccggctccc caccgccgag
     7021 ctccagcgcc tctcggaggt gctcaccgtg acgcaccggc agctggtcga tgcccggcag
     7081 cgagcggcac ggctcgaggc atcgcgccgc gagctcgtgg cctgggtctc ccacgacctg
     7141 cgcagcccca tcggttcggt gcgggcgatg gccgaggcgc tcgaggacgg cctggtcgcc
     7201 gagcccgccg atgttgcgtc ctaccaccgc gccatcgtcc aggaggtcca gcggctcgcc
     7261 gccctggtgg acgacctgtt cgagctgtcc cgcatcgagg ccggggcgcc gccgctcgac
     7321 gtgcccttcg tgcccctgca cgagctggtc gccgacgtca tcgaggccgc ccgcgtgcga
     7381 gccgacgccc agggcgtcgc gctgcacgcc gaggtccacg acctcggtcc cgagctcgtg
     7441 gtggctgccg acgtgcgccg cgcgatcgac aacgtcgtcg acaacgccat ccgccacacg
     7501 ccggccggtg gctcggtgac ggcgacagct cgagccgacg gcaccgggct ggtggtcgtg
     7561 gtgtgggacg agtgcggtgg catcccggcc tccgacatcg atcggatctt cgacgtggcc
     7621 ttccggggag acgactcccg gtcgagcgac accggcggag gaggcctcgg actggccatc
     7681 gcccgggggt tggtggaggc ccgggccggt ggcatctcgg tgcgcaaccg cgagcccggc
     7741 tgcgagttca ccgtccgcct gcccgccgag gtgccgtcgt gaccggtcgg gccgacgatg
     7801 cgtcgcgcac ccgacgggag cggcggtggc gcgccgccgt cgccggtgtg atcgctgccg
     7861 gtggggcgct cgccgccggc gaggtgccgc cggcgctcgc aggcgaggcg gagggaccgg
     7921 tgaccgccgt gggcaacgag ttcgtggacc ggttcgccgc gtcgctgaag gagctggccg
     7981 tcgccctgtt cggccagaac gacaaggtcg ccctgatcgt cggcatcgtc gtcatcagcc
     8041 tgctgctcgg ggcggcgttg ggtctcctcg ccctccgttc cttcgtcctc gcggccctgg
     8101 gcttcgccac cttcgccgcg atcggcctcg ccgcgcaggt cgccgacacc ggtgccggcg
     8161 tgctcgtgcc ggcgctgtcc gcgctcgccg gagcggcggc aggcgccggg gtgctggcgc
     8221 tgctggtggc ccagatcggg cgtgcgcctg acctggccac gacgttgcgc cgcgcgggcg
     8281 acgtcgagga tccgagggtg cggacaccgg atcggcggac gttcctggcc gtcggcgccg
     8341 cggccggcgc ggcggcggcc ctcaccggcg caggcacgtg gtccctgcgt ggccggtccg
     8401 ccgtcgactc ccagcgagcg gcgctggtcc tgccccggcc cgtgcgcgcc acgcccgtgc
     8461 gggcgagcgg tctcgagatc cccgggctca gcccctacgt gacgcccact gacgacttct
     8521 accggatcga caccgcgctc gtgacgcccc gggtggacgc cgactcctgg cggctgcggg
     8581 tctccggcct ggtcgaggag cccttcgagc tgaccttcga cgagctgctg gacatggaca
     8641 tggtcgagga gccggtcacc atcgcctgcg tgtccaacga ggtcggaggc gatctggtcg
     8701 gcaacgccgt ctggctcggc gtgcccctca ccgcgctgct cgaccgggcg ggggtccgcc
     8761 cggagggcac ccaggtggtc gggcggtcca ccgacggctt cacggccggg ttcccgacgg
     8821 acgtggcgac cgacgggcgg gtggccatgg tggccgtggg catgaacggc gagcccctgc
     8881 ccaccgacca cggctacccg gcgaggctca tcgtcgccgg cctctacggc tacgtctccg
     8941 cgacgaagtg gctgaccgag atcgagctca cccgcttcga ggacttcgac gcctactgga
     9001 tcccgcgcgg gtggtcgaag ctcggcccgg tcaggaccca gtcgcgcgtc gacgtgcccc
     9061 gcggcaccgt cgacgccggc acggtgccca tcgccggcgt ggcgtgggct cccgacatcg
     9121 ggatcgccgc cgtcgaggtg cgcatcgacg acgggccctg ggagccggcc gagctgcacg
     9181 gcaccgcgaa cgacagcacc tgggcccagt gggtccacca gtgggacgca accccgggtg
     9241 cgcacaccat ccgctgccgg gcgacggacg ccacgggcag cacccagacc gccgagcgca
     9301 gcgaccctgc accggacggc gcgaccggcc tccacaccgt ccaggtgacg gtgcggggct
     9361 gaccggcgcg gaccacccat cccaaccgga aggaaccagc catgcaccgc ctctcccgtt
     9421 cgacgcgcct gctcctcggc ctcgccgtgg gcatcggcct caccgccggc gcctgcgggg
     9481 acgacgacga caccgtcgag accgacccga cccaggaggt cgacgagatg accgaggagg
     9541 agatgaccga ggacgagatg accgaggacg agatgaccga ggaggagatg accgaggacg
     9601 agatggagga cgagatgacc gaggacgaga tgatggagga ggacatggag gacggaggct
     9661 gaccgacggg taggcgacct accgaccggt cggtaggctc cctcgagtga accgagtcct
     9721 ccccccggac gagcgcctga acctgcgcac gtcgatgccg ttcctcgcct tccacctgat
     9781 cccggtcgcc gcgatctgga ccggggtgcc ggtcgaggcc gtcgtgctcg ccgtggtgtc
     9841 ctacgtggcg cggatgttct tcatcaccgc cggctaccac cgctacttcg cccaccgcgc
     9901 ctacaagctg ccgcgctggg ctcaggcggt catggccttc gggggcacga ccgcgctgca
     9961 gaagggtccg ctgtggtggg ccgcccacca ccgcgaccac caccggtggt ccgacaccga
    10021 ccaggacgtg cactcgccga tccgtggctt ctggtggagc cacgtgggct ggatcctgtg
    10081 cgacaagtac tccgccacgg acgagtcacg gatccgggac ttcgccaagt acccggagct
    10141 gcggtggctg aaccgcaacg actgggtcgg tccggtgtcc ctcgccgtcg tgagcttcct
    10201 ggtcgccggc tgggccgggc tcgtcatcgg cttcggtgcc tcgaccatcg ctgtctggca
    10261 cgggacgttc ctggtgaact ccatggccca cgtcgtgggt cgccgccgct acgtgacgtc
    10321 ggacagcagc cggaactccg cggtcatcgc ggtgctcacc ctcggggagg gctggcacaa
    10381 caaccaccac cactaccagg ccagtgcccg ccagggcttc cgctggtggg agtgggatcc
    10441 gagcttctac gtgctgtggg cgctcgagcg ggtcggcatc gtgcgcgacc tcaagcgacc
    10501 ctcccggcag atcatgcagg cggacctggt cgaggccggc cacttcgaca tcggcatgtt
    10561 ccggaccggc tggcagcgag ccgccgcccg cctggtggcc gtgggtgacc acgccggcga
    10621 ggccctcgcc gagcgacgac aggccctcga ggaccgcatc gaagccgtgc gccacgacct
    10681 cgatgcgcac cgtcgggccc agcgcgacgc gatggccgag ctcgccgccg tccgccgagc
    10741 cgctcggacc ggtcgcccgc tcggcgcctg agcgccgggg acggcccggc gtcgccgatt
    10801 tgggtggtgg ggtcgggccg gtagcatctt ccaaagatct ttggaggatt ggcaatggct
    10861 gctcgaaccg ctcgtacgaa ccgacgtcgc acccgcacgg tgctccccat cgtggtggcg
    10921 ctcgccgccg cggtcgtggc catcgtgatg gcggtgggtg ccggcggggc gtcggacgac
    10981 acgacccgcg acgtcgccgc tgacgcgccc cgcgccggcg acgcacccct gaccggaccg
    11041 gagatccgtg gggcggggcc gatggcgaag ggagccgacg acgccccggt cgtgatggtg
    11101 atgtactccg agttccagtg ccccttctgc ggcaagttcg cgcgggacac cgagcccgag
    11161 ctggtggagc gcttcgtcga ggacggcacc ctgcggatcg agtggcgcga cctgccctac
    11221 ctcggtccgg agtcgctgca ggccgcggtc gccggacgcg ccgcggccga gcagggtgcg
    11281 ttctgggagc tggccgacgt gctgttcgcc gaccaagcgc cgcccaacag cggccgcctg
    11341 acggcggagc acctggtcga ggtggcgagc gaactgggcc tggacgccga gcggttccgc
    11401 gccgacctgg cggacgagga cctggtcgag gcggtccgcg ccgacgtgga cgaggccgtc
    11461 ggcttcgggg tgaccgggac ccccgcgttc ttcgtgaacg gccaggtcat catgggtgcc
    11521 cagcccgtcg aggtcttcgt ggaggccatc gagcaggccg cagccgcggc gggctgagcc
    11581 ccgatgtccg aggtcaccct cctcgccgcc ttcgccgcgg gcacgctcgc cctgctctcg
    11641 ccgtgcagtg cgctgctggt cccgtcgttc ttcgcctacg cgttcgggac gcgcaccgcg
    11701 ctgctcgccc gtaccgccct cttcgccctc ggcctggcgg ccgtgctcgt gcccctcggc
    11761 atggggtcta gcgccgcgtc gtccctggtc tacggcaacc gtcagaccat gatcgccgtc
    11821 gccggctggg cgatcatcgc gctcggcgtc ctgcagctgc tgggcaaggg cttcaccctg
    11881 cccttcgtcg gtcgcctgca ggcccggacc gccggcatcg acgcccgcgc cggcgcgctg
    11941 cccaccatcg cgctcggcgc cgtctacggc ctggccggct tctgctccgg cccggtcctc
    12001 ggcgcgatcc tcaccgtcgc cgccaccgag gaggccccgg cccgcggcgg catgctgctc
    12061 gccgtctacg ccctcggcat ggccgcgccc ctgttcgtgc tggccgcgct gtgggatcgc
    12121 ttcgacctcg gccgccgcca gtggctccgc gggcgacccg tgcacctcgg acccgtgcgc
    12181 acccacacca cgtcgctgct gtccggcacg ctcttcgtcg tcatcggggc gctgttcctg
    12241 agctccgagg gcatggtcgg gctgacgggc gtgttcggcg acaccaccga cctcgagttc
    12301 gatgcccagg cctggatcag cgaccggctg gcggtcgtcc cggactggtc ggtaccggcg
    12361 ctgatcgccg tggtggctgc gggggtcgcc ctccgtcggg ctcgggacga ggcgcccgag
    12421 caggtcgagg gctgagggtc gaaccgctca gcagacgaag cagatcggcg ccaggaacca
    12481 cagcgcggcc ccgcccagca ccatgccggt ggccgccgtg gcaacggtct ctaccgcgcc
    12541 cggttcatcg ccggggcgcg ccgcctcgtc ctcggtgggt gcgtcgccgg gcgtgggtgg
    12601 ggtctcggtg gtcgtcatgt cgtctccagg tcaggtgtcg gatccaccgg caccggcggt
    12661 ggccgggacc gggcaggcca gctggttgcc caggcgggtg tccagcgccc acagcagcag
    12721 gcccatggcg gcgagctgca gcagcggctg gacgggttcg aaccaggtga tggcgccggc
    12781 gctgcccagt gcgatcagca cgagcttgtt gcagaccggg cagccgacgg cgaagaaggt
    12841 gagcacgccg ccggccatgc cccggcgggc atcggggtcg acggtgtcgg cggggtcgcc
    12901 ggtgccgacg taggtggcgg ccagcagccc ggccagcacg gcgctgacca ccagggaggg
    12961 ccaggaccac caggtgggtg ggatctcccg accgaagaag gggttcggca ccaggtcggt
    13021 ggggatgccc acgacgagca cgtacgcggc ggtcgcggcg gcggccaccg cccagcgtcg
    13081 ggtgggccac gccagcaccg tcccccagcg atcggcgagc gtcatcgccc ggctccggcg
    13141 gccacgggca ggccggcggc ccgccactcg agcatcccgt cctcgaggcg caccgcgctg
    13201 cggccctcgg cggtgaggag gcggacggca tcgtgggcca gcacgcagtg cgccccccgg
    13261 cagtacgcga cgatctcgac gtcgtcgtcg acctcggcga ggtgctcgag caggtcgccg
    13321 aacgggaccg agcgggcacc ggggatgtgc gcggcctcga actcctcggg cgggcggacg
    13381 tcgaccacca ccacgtcgcc ggccttcatc cgccggcgga gctccttgcg ggtgatgagc
    13441 tccacgtcgt cgagcgcgcc ggtgccgaag taggtcgcaa gcgcccgctc gacgtccgcc
    13501 gagtgggtgc gggcgacggc gccgagcgac gcgtagagcg tggccacgtc gtcgccggcc
    13561 agccggtaga agaccctcgt gccctcggtg cgggtggcca cgacgttggc cagcttcagg
    13621 atctgcaggt gcgcggaggc ggtggtgacg ccgagcccga cctcgcgggc gatcgagtcg
    13681 accgagcgct cgccctgccc gagcaggtcg atgatcttga ggcggttcgc gctggcgaag
    13741 gccttgccga cgaccgcgag gcggtcgaac agctcgctgc ggtgctgggc gtcgtccact
    13801 gtgtcctcca aagttccttg gaagatgcta gcgggaggtg cgtgggaggg cgatccgggg
    13861 ccgacgccgg ggacgttagg ttcgccccgt gggcgacccc ggaacgcgat acgacctgag
    13921 cccggccggc atcgacgcga tgctcgaggc cgagttcccc gggaccggga actcctgcac
    13981 cgagctcggt ccgacccacg ccgtggcccg ccggccggtg gacagcgcgg cgatccgccc
    14041 cggcggcttc gtctcgggcc ccacccagtt cggcctcgcc gacgccgccc tctggtacct
    14101 ggtgttcggc gccatcggcc gcatcgagcc gatggcgctc acctccgagc tctcgatccg
    14161 cttcctgcgg ccggctcagg ggtcggtgct gtgggcccgc gccgacctcg cgtccgcggg
    14221 cagccggagc atcgtgggca gcgtgacggt ctggtgcgac gaccgcgacg acaagccaag
    14281 ctccgtcgct caggggacgt acgtacttcc gcgcgctggc tgatctcaga gcggcgaggt
    14341 gcggtaccgg ctgaggaagc ggtgctcgga ctcgctgtcg ccgacgagga ccagctcgcc
    14401 ctcggccggc aggggcacgg tggggtcggg gttcggctcg aactggccgt cggagacgcg
    14461 ggcgacgacg gtgcagccgg tctcctcgcg gatgcgggcc tgggtcagcg gcttgccggc
    14521 cagcttccgg gggacgggga cccggaacac gtcgaggccc tcggcgagct ggagggtgtt
    14581 gtcggcgctc agcatgttcc agatggcgtt ggcgccggtg gaggcgtagg agagcacgaa
    14641 gtcggcgccg gcgcggtgca gcgtcgagac gttccgatcg aggcgggacc gggcgatgat
    14701 ctgcgcgtcg gcgcggagcc gccggcagta gttggtgagg tacacgttga cgtcgtcgtc
    14761 gtgggtggtg accacgaccg acggtgcgtc gtcgatgccg gcccgcttca gcacggcgat
    14821 gtcggcggcg tcgccctcca cgtagtcggc gccgtcacgg atccggtcgg actgcttctc
    14881 gacgatgcgg ttcgggatct ccatcgcccg caggtgccgg gacacggccc gtcccacccg
    14941 gccgccgccg atgatgacga ccggcgggtc cgccgagcgg tcgggctggt cgtcagccgc
    15001 gaagcgctcg tcgtatcggg ccagctgctc gatggagccg gcgagcacga ggatgctcgt
    15061 ctcggtgagc accgtcgatg gccgggcgat gagcagctca ccgcgatccc acaccccgcc
    15121 cacggtgagg ccggtgtcgt cgcggatgcc ggagccgagg agcggcttgc cgatgagcga
    15181 gtccggcgcg gcggcctcgg cgatgagcag ctcgtcgaag cgcccgacca cctggctccg
    15241 gccgtcgggt gccagcaccc gtcgggcgag ggcggcgccg agcatctcgc cgaggcgcag
    15301 cacctcgtcg cagccggcga gctcgaggat gtcgatggac gcctcggcgt tggcggtggc
    15361 cacgatccgg atcgactcgg agatctcccg gacggtgaac gccacgttgg tgttcgtcgt
    15421 gtccgcctgg gtcgtcgcga ccagggacgc gcgctcgacg ccggccgcgg cgtaggtcgc
    15481 ggggtcgtcc agctcgccga ccatgaccgt caggccctcg tcgtggtgcc tgagcgcctc
    15541 gtccaggtcg gcgacgaggg tgacgtgcgc gatccccgcc cggtcgagcc gggtccgcag
    15601 cgcggcctcg atcggcccgg tgctggtgag gatgacgtgg tccgagacgt cgtcgtccac
    15661 ccgccggggt gcccgggccc gctgggtcga ctccatccag ggcaggaaca cgaactggat
    15721 gaacgtgaac ggcaggagca ccaggatgaa caccgacccg gacagcagca ccaccaccga
    15781 gaaggtgcgg cccaggtccg attcgaaggt gatgtcgccg aagccgaggg tcgacatcgt
    15841 gacgagggtc cagtagatcg ccgtgatcca gctgtagcgc tggccctcgt gctccatgat
    15901 ctcgtggaag agcgcgctgt acacggccac gatcacggcc agcagcgcca gcatccaccc
    15961 gaggatcttg gcgctgcgcc gggcctggct ggtggccagg tagctgagga tgaacccgat
    16021 ggacttcacg tcgggcgtcg ctccggacgg ttcgacatcc gacgatcctg tcaggacccg
    16081 cgcaggagct ggtcgacggg ggcgtggtcg tcggtgagga cctcggcgcc atcgatgtag
    16141 ctgcgcacga acgcctcgtc gcgcaggacg ccgtcggcgg ggtcgatgtc gagatccgcg
    16201 atcggctggt ccgatgccac caccacctgg ttgcggggcg tgtcgggtgg ctcgccgccg
    16261 gggaggatca cctggacgtg gtcgaacacc tcgagcagcg tcgccagctc ggcccgggcg
    16321 aacgcgctgt cgccgccgtc gatgacgttg agcacataga tgccgccgtc ccggaggacc
    16381 cggtccacct tcccggcgag ctccaccgtc gtgaggtgcc acgggacgga gagaccgctg
    16441 aacgcatcac ccacgacgag gtcgtaggca tcgtcggcga gccgggggat ctccgtgcgg
    16501 gcgtcgctga ccgccaccgt gaggtcgggg ccggtctcca ggccgaggcg gtcctcggcg
    16561 atgtcgacca cgccgggatc gatctcgatg acgtgggagc tggatcccgg acgctccgcc
    16621 gccaggtagg tcgggacggt gaacccgccg cccccgatgt ggagcgcgtc gatcggcccg
    16681 ggcgggaggg cctcggccac gtcggcgaac aggcggatgt accgcaggtc caggtgcgtg
    16741 gggtcgtcga cgtccacccg ggcgtggtgg agggtgtcga ggtacaggct gcgccgggac
    16801 gggtcgtcct cgtcgacgac caccgaggcg caggcatagc gggtctcgac atcgcactcg
    16861 ggcgggaacc cgtagacggc gagtccgctg acgacgaaca ccaccgcggt cgacgccgcg
    16921 ggcagggacc gccgcagcgc ccactcgagc agcacgcccc cgaccacgag cagcaggccg
    16981 acgacgaaca tgatcgtgcg ggtgccgagc agggcgacga ggacgaagcc ggtgaggaac
    17041 gtgccggcga ggcccccgaa cgtgccggcg gccgagagcc caccgaacac cgacccggtc
    17101 tcctccaggg tggacagccg gagcttggcc acgatcggcg tgctggcgct gagcacggcg
    17161 gccgggagga agaacgccat cgtggcgagc accacgatgt ccagcgggtt cccgtcgact
    17221 tcaggaccga tggcccgcac gatcggcacc gcgatccaga cgagcgcgcc acccacgaac
    17281 agggagggcc cgaggagccg ccgggggtcg tggcggtccg ccagccagcc cccgacgccg
    17341 gcgccggtgg cgatgccggc gaggatcacc ccgatgatgg cggtgaacgt ctcgagggac
    17401 accccgacgt agggcgccag cagccgcccg gcggtgatct cgacgatgag gatcgccgcc
    17461 gaggtggcga acacgacggc gatggcgagg cggcggccca tccggccatc ttggctgccc
    17521 ggcagcgccc ccacgaggcg ggcgtagagt gcgccccgtg ctcctgacct ccaccacgac
    17581 ggacccggcg gccttcgccg ccgccaccgg ctgggccgtc aagcccgagg gggcgtgccg
    17641 ggccgacatc tgcgtgccgc tgcccgacga ggtccgggtc gacggtggcc tcgacgtcac
    17701 cgtgctggcc gaccgcctcg gcatgccggt gctccgcgac gacgaccacg gcctcgtggc
    17761 gctcggtccg gccagcgtcg tcggccgagc cctcgagacc gcggaggcgc ccgagctcga
    17821 gctccccgac gccgacggca acccgttccg gctgtcctcc ctgcacggca agaaggtgct
    17881 gctggtcgcc tgggcgtcgt ggtgagggtg ccgcgatgac ctgcccgtgt ggcaggcact
    17941 gcgcaccgag ctggctccga agggcgtcga ggtcgtcacc gtcgcgctcg acaccggcgg
    18001 ggtcgcggcg gcgggcccgt ggatcgcgaa ggcgtcgccc gagcacccgt ccctgatcga
    18061 cgaggcccac gtctcggccg agcgcttcgg cttcgtcaac gtgcccaacg ccgtgtggat
    18121 cgacgaggac ggcatggtcg tgcggccctc gcacccggcg cacgtgaagg tctccaagct
    18181 ccgcgagatc gaggtcaccc cggacatggg ctacctcggg gagatgctca ccgagctcca
    18241 gaagttcaac gtcgacgcca ccacgtacct cgacgccatg cgcgactggg tcgagcacgg
    18301 cgccgacagc cggtgggcgc tctcgcccga ccaggtcgtg gacgcgtccc gcccccgccc
    18361 gcgcgagcac gccgaggccg cggctgcgtt cgagctcggc cagcacctgc accgggccgg
    18421 ccaccccgac gacgcggtgg cctggttcaa gcgggcgcac gagctgcacc ccgagaactg
    18481 gacctacaag cgccaggcgt ggaccttcgt gaccacgccc gagggtgagg cgagcgacct
    18541 catccaggcc gacaacgacg tgtacggcac ctcctggctg gacgaggtgc gtcgcctcgg
    18601 ccccgagacc tactacgagc ccttcgacgc ctgagcgtcg ctcggctgca ccaggccagc
    18661 ctggccgggg aagacccggg ccaccttgtc gctcaggtac ccgccgtagg tgccgtccca
    18721 gtcgaacacg gaccggccgt cccagcgcgg cggcgggggc gagccggtgt cgccgaagcc
    18781 gggcacgggg acgatgcgcg cgtcccagga cgggtcgagg aagaagggga acgacaggcg
    18841 cccgcgcgac ggccgtcgca cccggtgcgg ggtggaccgg taggccccgc ccgtcacccg
    18901 ttcgagcatg tcgccgaggt tgcagacgaa ggagtccggc accggcggcg cctcgaccca
    18961 accgcccggc gtgcgcacct cgagaccccc cacgtcgtcc tgggccagca gggtgagcag
    19021 gccgtagtcg gtgtgctcct ggacgcccca cgacgcgtcc ggcacctccc cggccggggg
    19081 gtagtggaag atccggaaca ggatcgtggg atcggcggtg aggtgccggc ggaaccagtc
    19141 ccgctccagc ccgaggccca gggcgacgag ctcgagcacc ccctggccga ggtcggtgag
    19201 ccggtcgagg acctcgagga cgagcggggc cagctcggcg ggctcgtcgg ggaagaggtt
    19261 ggcgccgtgc agcggcgtgg cggcctggac ccgggggtgg tcggggccgt cctcccgccc
    19321 gaagtagatc ccctccttgc cgtcggggac gccgccggtg agctcgccgc ccggcgggaa
    19381 ccagccgcgc caggccgcgc cggcgcgggc catggcgatg cgctccttct cggcggcggg
    19441 ccgggcgaag aagtcccggg cggcccggtc gagcgcagcg agcagctcgg gctcgatgcc
    19501 gtggccggtg acgacgaaga acccgtgctc ccggcaggcg gcgtcgatcg ccgccgcggc
    19561 caccgtggcg tcgccgccgg cgagcgcctc ggcgcagtcg atgacgggga tgccggtcgt
    19621 ggccacgtcc cgagtctccc cgatccggac cggttggggt ggcgtcggcg ccggccgtac
    19681 cctcgcccgg tggagtcgag tccgggcacg agcgagctgc gcgccatcga ggccgaggtg
    19741 cgggcgcacc cgcacggacg ggttccccgc gagctgcggc gccgccagct cctggccgtg
    19801 gccgagcagc tgttcgtcga ccggggctac gccgccacgt ccatggacga cgtggccgcc
    19861 ggtgccggcg tgtccaagcc gatcgtctac gagctggtcg gctccaagca cgaggtgttc
    19921 gaggcctgca tggcccgggc cgccgacgcc ctcgccgccc gggtggccga cgccgtgctc
    19981 cacgccggcg agggggatgc cacccgcctg cgggccggcg cgctggcctg gttcgagttc
    20041 atcggcgagc gccgcagcct gtgggacgcc ctgctcgcct ccgacgacgc tcccgcctcg
    20101 gccgccatcg aggcggtccg ggcccgccag gacggcttcg tggccgccca gctcgccgcc
    20161 accgcacagg ccgggggagc ggcggtcgat cccgagctgg tcgaggccgt cgccaccgcc
    20221 atgaacgggg cgttcgaggc gctcggccgc tggtggagcc ggcaccccga ccgcagcgcg
    20281 accgagctcg ccgacctgta caccgcgctg gtgctgcccg gcctcgaggg tctgctcggc
    20341 tggtccccgt agcccggtga cgggcaacgg gcgttgccag gtccgtcagt ggtggtagga
    20401 agaaccacct atgaagctct ccatgaccct caactactcg ggtgacccgg tgcaggccgc
    20461 cgccactgcc gccgactacg agaaggccgg cgtggacatg atctgggtgg ccgagctgta
    20521 cagcttcgat gctgtcagca tcctcggcta cctcgccgcc aagaccgaga cgatggagct
    20581 ggcgtcgggc ctcttcccga tctactcgcg gaccccggcg ctcaccgcct ccaccgccgc
    20641 cggcctcgac gccgtcagcc agggccgctt cgtcctcggc ctcggcgcgt ccggccccca
    20701 ggtcatcgag ggctggcacg gcgtcaagta cgacaagccc ctcacccgca cccgcgagct
    20761 catcgacatc tgccgcatcg tgtggcgtcg cgagcgcctc gagtaccagg gcgacgccta
    20821 cacgcttccg ctcccggccg accagggctc cggcctcggc aagccgctga agctgatcaa
    20881 ccggccggtg cgcgagaaca tccccatctg ggtggccagc ctcgggcaca agaacgtgtc
    20941 catgaccgcc gagctcgccg acggctggct gccggtgttc ttccaccccg acaaggcccg
    21001 cgacgtgtgg ggcgacgacc tcgacgacgg cttcgccaag cgcagcagcg acctcggccc
    21061 cctccagatc gcggcgggtg gcacggtggc catcaccgac gacaagtccg tggccgacgg
    21121 catccgggac ttcgcccggc cgatgaccgc cctctacgtc ggcggcatgg gcgccaaggg
    21181 caagaacttc tacaacaaca tcttcaagcg ctacggctac gaggaggagg cggagaagat
    21241 ccaggacctc tacctcgacg gcaagaagga cgaggccgcc gccctcgtgc ccgacgactt
    21301 cctcgacgcc accgccatgg tcggcgacga gggctgggtc aaggagcgca tccaggccta
    21361 caaggccgcc ggggtcaccc acctgcagat cacgccggtg ggcgacaagc ccctcgagat
    21421 catcgagaag gtgaaggcct gggcggagtg acccgccggg ccgccatctc ggcggccagc
    21481 cggtccgcgt cccactcgca gccggtgacc caacccgacg ccgacgtcac gccgagcacc
    21541 gcgagccgcg ggagctcggc gtcgtcgtcc acctgggcga tgcagggcac gtcgagggtg
    21601 gtggccagcc gcgtcgccag ggcgatcgcc ttcgggttct cgccggcgtc gcggaccagg
    21661 gtcgggtcga gctcgacggc cgcgaggggc agcgacgcga gcaggccgag gggcagccca
    21721 cgccgcccgt gcccgctgag caccaggcgg agcccgtcgc gaccgagcac gtcgatgacc
    21781 tcctgaccca tcgcggtgat gtccaggacg ctgtgcgcgt cgacccggag gtgcacctcg
    21841 gcgtcggggg ccagctcccg cagggcgcgc aggcggccga ccacgtcctc gtcgcggagc
    21901 tgggaggtgg ccagcgtgat gtggatgggg acggtcgact gcgcagccgc cgcggcgcgg
    21961 agcgcgtact gggtggccgc ccaccagatg aggtccgcgt acccggtgac cagcgcctcc
    22021 tcggcgaagc tccgggccgg caggcgaccg aggtcggggt ggtcccaccg agcgagcgcc
    22081 tgcagggcgc acagctcttg ggatcgcagg tcgatgaccg gttcgaagcc ggcgacgacc
    22141 tgaccctcgt ccagcgcccg ggcgagctgg tcgcccatgt cctctgcgtg gccgaggatc
    22201 ccggagtggt cgttcccggc gacccgcacg cagttcccgc cgctgcgctt ggcccggtac
    22261 atgccccggt cggcggcggc gaggagggcg tcgggctcgg cgtggacccc cgggttggag
    22321 acggtgagcc cgatgctggc ggtgacccgg atccggtgct cgccgatcac gtagtcgagc
    22381 atcccgatct ggtcccggat gcgctcggcg acggccatcg ccgccgactg ctcctccggc
    22441 gggtgcacga tgaccacgaa ctcgtcgccg ccgaggcggg ccaccgtgtc ggccggtcgc
    22501 acggcggact ggatccgggc cgtgacctgc tggagcagtc gatcgcccac cgcgtggccg
    22561 tgctcgtcgt tgaccgcctt gaacccgtcg agatcgaggg tgaggaccgc cacgggtgag
    22621 ccgagacggg tggcgatgga cacggcgacg tcgaggcgat cgagcagcag cacccggttg
    22681 gccaggccgg tgaggggatc gtgcgacgcc tggtgggaga gcgcggcctc ccgctcgtgc
    22741 tgggaggtga cgtccaccgc cagcgccccg atccagcgca cctcgccgtc ggagtccacg
    22801 tcgccggtga gcacgacgtc gacgaccccg atggtgccgt ccgcccgccg caccggcagc
    22861 tcgcccgtcc acacgccgtc tcgcaccagc gccgggcgga tgctggcgta gtacctgtcg
    22921 aagaccacgt cggtgaacag gtccgcggtg ctcagccgct ggccgggtcg gacaccgacg
    22981 aagcggcggc cggcctcgtt gagccacagc acctcgcccg aaccgtcggt catccccacc
    23041 aggtggggca tccgccgcac cagctccagc acgcgcggag cccagtgcag gtcgagctcc
    23101 gcgacgtctg ggggtggtgt gggcaagtgc tgtggtggcc ccgctggcct gttgtcggtc
    23161 tgtgatcggt gctgtaccca gggtgacggg tgcgtggacc tcgcggctac ggtcgattcc
    23221 acccggacgg gaggggacgg cagtggacga agcaaccaac gggccggggg acgggtccgg
    23281 cgcgccggcc gggccctacg agcgcctcgg cgtgctggct cgccagcagg tcgaggtggc
    23341 cgacgggttc cgccacgtcg agctgttcac catgcagggc ctgctcaccg tcctctggca
    23401 cgggccggtc gaggccgatc gagccgtgat catgctgggc ggtgcgatgg gcggcctgct
    23461 cggcccggcc gacgggctct accacgacct gggcgtcgcc ctcgccgacc aggggatcgc
    23521 caccttccgg gtctcgtacc gcaaggccaa cgacttcggg cgctgcgtgc acgacgccct
    23581 cgccatcgcc gacgtcgccg cccggcaggg cgcacgccgc ttcgtcaccg tggggcacag
    23641 cttcggcggc gcggtggccg tccagctcgg gatggccctc ggcgaccacg ccggcggggt
    23701 ggtcacgctg gccacgcagt ccgccgggtg cgaggacgcc gacggcctcg ccgacacccc
    23761 gatgctgctg gtgcacgggg atcgggacga gatcctcccc gccttcgcca gcgaggtggt
    23821 gcacggcctc gccggcgggc acggcgagct gatgatcctc cccggcgccg gccacctcct
    23881 gacggaggcc ggcgacgagc tgcgggcgaa gctcctcgac tggatcccca cccacctcgc
    23941 ctgaagttcc catccagcgg actgcactcg atgccggtgc cgtctacagc acccgccgca
    24001 gcgacggcgc caggagcagg ctggccagca gcgtcaccgc ggcggcgaac aggaagggcg
    24061 cctgcatgcc gaggccgtcg ctgatcgccc cgccgaggag tgctccgacg gtggcggcgg
    24121 tccacagcag cgagcggatg gcgccctggg cccggccgag caggcggccg ggcacgctgc
    24181 gctgccgggc ggcgatgacg acgatgcggc cggtgacgac cgcggcgaag gacagcgcga
    24241 agccgaccgc gaccaccggc agcaccggta cggccatgag gaggaacgac acgacggtgg
    24301 cggcgagcgc gccgagcagg acgtggaagg gccggtggcg cttgacgagg cgcttgacgc
    24361 tgcgggcccc ggccagaccg ccgagcgacc cgatgctgag gaagatgccg tagccggtgg
    24421 cgctgaggtc gaggcgctcg gtcaggtaga tgacgatcgg ggcgatggct gcgatgctgc
    24481 cgagctgcag cagcacgatg agggcaccgg tgctgcgcag gaccgggttg gtccaggaga
    24541 tcctcgcccc ctcgcgcagg tcggcccacc aggtgctggc gggcgggtcg tgggtgtggg
    24601 actcgtccac gtcgacggtg cgcacgagcg caccggcgac caggaaggac gcggcgtcga
    24661 ccacgaacgg cgccgagctg gacagtgcgg tgagggtgcc accgatggac gggccgacga
    24721 ggtcgttcgc cacgagctcg gcggtctcga gctcgccgtt ggcctcctcg aggcggtcgt
    24781 cgtcgaccag gtcggggagc agcgccgggc tggaggagtc ggcgagcgcc tcgccgcacc
    24841 cgagcaggaa ggcgcccccg acgagcacgg cgaccgtcgt ggcatcgagg aggatcaccg
    24901 cggccagccc ggccatcacc acgaagcgga acaggtgcgc cgagacgatg agcgggaccc
    24961 ggcggcggcg gtcggccatg gtgccggcgg ggaggccgag cacggcgaag ggcagggtcc
    25021 cggcggcggc caccaccgcc acggtcgtgg cggacccgtc gatcccggcg gcgatcaggg
    25081 gcagggcggc gttgcgcacg ccgtcgccga ggttcgacgc ccacgccgcc gcccgcaacc
    25141 gctggaaggg cgcggggagc ttcggatcga tccgcaggtg ggctcccatt ctccgatgat
    25201 cttcccacag cggacctccc gatccggaca cggtgcgacg tcggtttgcg gaccaccggg
    25261 gtcgcgggag aaactcggga tcgcccgcga tcccccctcg gaggacctca tgaccaccgt
    25321 gaccatcgcc ggccaagagc tgcatgtggt cgaccgtggc aacggtgacc cggtggtgtt
    25381 cctccacgcc ttcccgttca acgcggcgat gtgggagtac cagtacgccg cgct
//
LOCUS       DATISU010000293         1857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_18982, whole genome shotgun sequence.
ACCESSION   DATISU010000293 DATISU010000000
VERSION     DATISU010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18982"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="VLR27_11855"
     CDS             complement(<1..1200)
                     /locus_tag="VLR27_11855"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSP04193.1"
                     /translation="MRDVLDPSTGAVVDRVPELGPGDVDAVLTRTVDAHADWSRSGVR
                     VRRDVLREVARLIRVNAEHLARLESAQAGKPISAARGEVGAAAEVFEFYAGAVDKVHG
                     STIPGNADGTLLTFREPIGPSVAIVPWNFPLLILCWKVAPALAMGNAVLAKPASQTPL
                     TAIALAELAEGAGLPPGTFQVVTGPGGSLGEALVTDPRVRKVSFTGSTEVGSRIMALA
                     ARDITRVGLELGGKSASLVFDDLSDSQLDACVASSLWSVFDNTGQDCCARSRMFVHES
                     VVDDFTERFVAATEAIRIGPTGDGDTELGPLISDDQRAIAEEYVASAQADGARLRTGG
                     NRPDLPGSYLAPAVLDRADVGMRFMREEIFGPVVGIQSFRTEDEAIALANASIHGLSG
                     TIWCRDVG"
     gene            1264..>1857
                     /locus_tag="VLR27_11860"
     CDS             1264..>1857
                     /locus_tag="VLR27_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04194.1"
                     /translation="MSDTAKKPHHHKKVVTVLEPEDEYTHEPDEAENYNESMYLNAFD
                     AAQEVGGWFRLGNRVNEGYAEMTVCVYLPGGRVGFVYGRPQIDTNDKMEAGGLSIEVV
                     EPFKHLKVRYQGKVCLLDEPGQMADPRTAFKENPWVECEVDLDYRGVSPMYGGKPQYE
                     DGTEVEVEAEKSFAKAHYEQHCAVTGTIRVGEETIDMD"
BASE COUNT          300 a          620 c          699 g          238 t
ORIGIN      
        1 accgacgtcg cgacaccaga tcgtcccgga caggccgtgg atggacgcgt tggccagggc
       61 gatcgcctcg tcctccgtgc ggaacgactg gatgccgacc accgggccga agatctcctc
      121 gcgcatgaac cgcatgccga catcggcccg gtcgaggacc gccggggcca ggtagctgcc
      181 cgggaggtcg gggcggttgc cgccggtgcg gagccgggcc ccgtcggcct gggcggaggc
      241 cacgtactcc tcggcgatgg cgcgctggtc gtcggagatg agggggccga gctcggtgtc
      301 gccgtcgccg gtggggccga tgcggatggc ctcggtggcg gcgacgaagc gctcggtgaa
      361 gtcgtcgacc acggactcgt gcacgaacat gcggctgcgg gcgcagcagt cctggccggt
      421 gttgtcgaac accgaccaca gcgatgaggc cacgcaggcg tcgagctggc tgtcggagag
      481 gtcgtcgaac acgaggctgg ccgacttgcc gcccagctcg aggccgaccc gggtgatgtc
      541 ccgggcggcc agcgccatga tccgcgaccc gacctcggtg gagccggtga aggacacctt
      601 gcgcacccgg gggtcggtca ccagcgcctc gccgagcgat ccgcccggtc cggtcacgac
      661 ctggaacgtg ccgggaggga gcccggcgcc ctcggccagc tcggccaggg cgatggcggt
      721 gagcggggtc tgcgaggccg gcttggccag gacggcgttg cccatggcca gcgccggggc
      781 caccttccag cacaggatca ggagcgggaa gttccacggg acgatggcca cgctcgggcc
      841 gatcggttcc cggaaggtga gcaacgtgcc gtcggcgttg ccggggatgg tggagccgtg
      901 gaccttgtcg acggcgccgg cgtagaactc gaacacctcg gcggcggcgc cgacctcacc
      961 ccgggccgcg ctgatcggct tgccggcctg ggccgactcg aggcgggcca ggtgctcggc
     1021 gttgacccgg atgaggcggg ccacctcgcg cagcacgtcg cggcggacgc gcacgccgga
     1081 ccgggaccag tcggcgtggg cgtccacggt ccgggtgagg acggcgtcga cgtcgccggg
     1141 gccgagctcg ggcacccggt cgacgacggc accggtggag gggtcgagca cgtcgcgcac
     1201 gggcgggaac cgtaccggtt ggctaggttg gcaacgcctg taaccaagtt ctccggggga
     1261 tccgtgtccg acaccgccaa gaagccgcat caccacaaga aggtcgtcac cgtcctcgag
     1321 ccggaggacg agtacaccca cgagccggac gaggccgaga actacaacga gtcgatgtac
     1381 ctcaacgcct tcgacgcggc ccaggaggtc ggcggctggt tccgtctcgg caaccgggtc
     1441 aacgagggct acgccgagat gacggtgtgc gtgtacctgc ccggcggccg ggtcggcttc
     1501 gtctacgggc gcccccagat cgacaccaac gacaagatgg aggcgggcgg gctgtccatc
     1561 gaggtcgtcg agccgttcaa gcacctcaag gtccgctacc agggcaaggt gtgcctgctc
     1621 gacgagcccg gccagatggc cgacccccgc acggcgttca aggagaaccc gtgggtggag
     1681 tgcgaggtcg acctcgacta ccgaggcgtg tccccgatgt acggcggcaa gccccagtac
     1741 gaggacggca ccgaggtcga ggtcgaggcc gagaagtcct tcgccaaggc ccactacgag
     1801 cagcactgcg ccgtcaccgg caccatccgg gtcggcgagg agaccatcga catggac
//
LOCUS       DATISU010000294         2659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_251669, whole genome shotgun sequence.
ACCESSION   DATISU010000294 DATISU010000000
VERSION     DATISU010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251669"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VLR27_11865"
     CDS             <1..699
                     /locus_tag="VLR27_11865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HSP04195.1"
                     /translation="DERWTDERKRLILESRTKSTDLIARTIAGLDRKPSVLLSASGID
                     YYPDAGDEVLTEESRAGEGYLTDVCVQWEGATAPAQDAGIRTCLLRTGIVQSADGGAL
                     KKTLLPFKLGVGGRFGSGDQWWSWISIDDHVRAMLHLIESDIEGPVNLTAPNPVTNQQ
                     YTKALGRELGRPTLLPIPKLGPTVLLGKELTEALLYTSHRVLPAVLEADGFTFRHPTI
                     EVCLAAMLGDDEAA"
     gene            complement(748..2223)
                     /locus_tag="VLR27_11870"
     CDS             complement(748..2223)
                     /locus_tag="VLR27_11870"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HSP04196.1"
                     /translation="MPAAPLPPRAGRLLLLGVLAASLVSCRASDVALEAPTTTTAAPA
                     TTTTTAPPATGEAGEPGVGDPYFPELGNGGYDVASYHIDLEWLADNGSIEATTTIELT
                     PVVDLRSFNLDLVGLDVSLVTVDGVDVGFRRSGREMTVLPPSALPGGEEVTVEVSYAG
                     RPQPISLGSDVFGAGWQTDGREAYVVSEPAGAASWVPANDHPTDKALFTFELTVPADQ
                     AAVANGVLVEERVEPDGRQTSVWRASDPMATYLASIVVADLVIERTTAPTGLPLRDAY
                     PPEHAAGAQELFARTGEMVATFEDWFGPYPFEVYGHVVVDEALGFALENQTLSLFGTD
                     LLGVGGAGERTVAHELAHQWYGNAVSPASWRDIWLNEGFATYAEWIWLQEAYGQPIAS
                     SAERAHAGADYGVAPGDPGNEELFQPTVYIRGGLALHAISVAMGDEAFRALLREWPQR
                     FHDSTASTDDLRALAEELSGQDLGPVFDAWVYGVALPPFPA"
     gene            2279..>2659
                     /locus_tag="VLR27_11875"
     CDS             2279..>2659
                     /locus_tag="VLR27_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HSP04197.1"
                     /translation="MSTVLVTGATGYIGGRLVPALLEAGHEVRCLVRTPTKLRGIDWA
                     ERVTVVQGSLEDDGAVREAMEGCAAAYFLVHSMGGSDDFAATDRRNDRLFAEAAADTP
                     TMERIVYLGGLGQEGEDLSPHLASR"
BASE COUNT          370 a          992 c          946 g          351 t
ORIGIN      
        1 gacgagcgct ggaccgacga gcgcaagcgc ctcatcctcg agtcccgcac caagagcacc
       61 gatctgatcg cccgcaccat cgccgggctc gaccgcaagc cctccgtgct gctgtccgcc
      121 tcgggcatcg actactaccc cgacgccggc gacgaggtcc tcacggagga gtcacgcgcc
      181 ggcgagggct acctcaccga cgtgtgcgtc cagtgggagg gcgccaccgc gccggcacag
      241 gacgccggca tccgcacctg cctgctgcgc accggcatcg tccagtccgc cgacggcggg
      301 gcgctcaaga agacgctgct gccgttcaag ctcggcgtcg gcgggcgctt cggctccggc
      361 gaccagtggt ggagctggat ctccatcgac gaccacgtcc gggcgatgct ccacctcatc
      421 gagtccgaca tcgagggtcc ggtgaacctc accgccccga acccggtgac gaaccagcag
      481 tacaccaagg ccctcggtcg ggagctgggc cgcccgacgc tgctgcccat cccgaagctc
      541 ggccccaccg tgctgctcgg caaggagctc accgaggcgc tgctctacac ctcgcaccgg
      601 gtcctgcccg ccgtgctcga ggccgacggc ttcacgttcc gccaccccac catcgaggtg
      661 tgcctggccg cgatgctggg cgacgacgaa gccgcctgat ccgttctggg acgcggtgca
      721 cgcctccggg gcgtgcccgg ctgcccgcta cgccgggaac ggcggcagcg cgaccccgta
      781 cacccaggcg tcgaacaccg gacccaggtc ctggccggac agctcctcgg ccagcgcacg
      841 gaggtcgtcg gtcgacgccg tcgagtcgtg gaaccgctgc ggccactccc gcagcagcgc
      901 ccggaacgcc tcgtccccca tcgccaccga gatggcgtgc agcgccaggc cgccgcggat
      961 gtagacggtg ggctggaaca gctcctcgtt gcccgggtcg ccgggggcca cgccgtagtc
     1021 ggcgccggcg tgggcgcgct cggccgagct ggcgatcggt tgcccgtacg cctcctgcag
     1081 ccagatccac tcggcgtagg tggcgaagcc ctcgttgagc cagatgtcgc gccacgacgc
     1141 cgggctcacc gcgttgccgt accactggtg ggcgagctcg tgcgcgaccg tgcgctcccc
     1201 cgccccgccg acgccgagca gatcggtgcc gaacagcgac agcgtctggt tctcgagggc
     1261 gaagccgagc gcctcgtcca ccaccacgtg gccgtagacc tcgaaggggt aggggccgaa
     1321 ccagtcctcg aacgtggcca ccatctcccc ggtgcgggcg aacagctcct gcgcgccggc
     1381 ggcgtgctcc ggcgggtagg cgtcgcgcag cgggagcccg gtcggtgccg tggtgcgctc
     1441 gatcaccagg tcagcgacga cgatcgaggc gaggtaggtg gccatcgggt ccgacgcccg
     1501 ccacaccgag gtctgccggc cgtcgggctc gactcgctcc tccaccagca cgccgttggc
     1561 gaccgccgcc tggtcggccg ggacggtgag ctcgaaggtg aacagcgcct tgtccgtcgg
     1621 gtggtcgttg gccgggaccc agctggcggc gccggccggc tcggacacga cgtaggcctc
     1681 ccgcccgtcg gtctgccagc cggcaccgaa cacgtcgctg ccgagcgaga tgggctgcgg
     1741 ccgaccggcg tagctgacct ccaccgtgac ctcctcgccg ccggggagcg cggacggcgg
     1801 gagcaccgtc atctcccgac cgctgcgtcg gaagccgacg tccaccccgt cgacggtgac
     1861 gagggagacg tccaggccga ccaggtcgag gttgaacgag cgcaggtcga cgacgggtgt
     1921 cagctcgatg gtggtggtgg cctcgatcga gccgttgtcg gccagccact cgaggtcgat
     1981 gtggtagctg gccacgtcgt agccgccgtt gccgagctcg gggaagtacg ggtcgcccac
     2041 cccgggttca ccggcctccc ccgttgccgg cggtgccgtg gtcgtggtcg tcgccggcgc
     2101 cgcggtcgtg gtcgtcggcg cctcgagggc gacgtcgctg gcgcggcacg agacgaggct
     2161 ggcggcgagg acgccgagga gcaggagccg gccggcccgg ggcggcagcg gtgcggcggg
     2221 cacggccagc atcctcccac gacgtcccat ccgggccgca ccgcgctaca cagagcgcgt
     2281 gtccaccgtc ctcgtcaccg gcgccaccgg ctacatcggc ggccggttgg tccccgccct
     2341 gctggaagcc ggtcacgagg tgcgctgcct ggtccgcacg ccgacgaagc tgcggggcat
     2401 cgactgggcc gagcgggtca cggtcgtgca gggctcgctc gaggacgacg gcgccgtgcg
     2461 cgaggccatg gaggggtgcg cggccgcgta cttcctcgtg cactcgatgg gcggctccga
     2521 cgacttcgcc gccaccgatc gccgcaacga ccggctcttc gccgaggccg ccgccgacac
     2581 gccgacgatg gagcgcatcg tatacctcgg cgggctgggg caggagggcg aggacctttc
     2641 accccacctg gccagccgc
//
LOCUS       DATISU010000295         7292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_299054, whole genome shotgun sequence.
ACCESSION   DATISU010000295 DATISU010000000
VERSION     DATISU010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299054"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="VLR27_11880"
     CDS             <1..1458
                     /locus_tag="VLR27_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP04198.1"
                     /translation="EQFGRPIAMYQAVKHHCANMAVARELTTSAVWDAARAAATGGDQ
                     LSYAAAVAATLAGPAADLCANLNTQVHGGIAITWEHDAHLYMRRATTLLTLLEPKQAA
                     AEIVYLVRAGVTRAKAVELPPEAEAIRDEVRTFADSIKDLSKDEQRTKLIETGYVMPQ
                     WPEPYGRAAGAIEQLVIEEEFAAAGIQRPGSGITAWNILTIIQYGTQDQLDRWVNPAL
                     HQDVIWCQLFSEPDAGSDAAGVKTKATRVDGGWLVNGQKVWTSGAHVAGMGFATVRTN
                     PDVPKHDGITMMAIDMHAEGVEVRPLKMTNGGSEFNEVFFNDVFVPDDDVVGPVDGGW
                     TVARSTLGNESVSIGGGGGGMSMPGAALVPPLDANPDRLTGGAARVGRYIAQHQAMGL
                     LNLRAANRAVAGGEPGPEGAMTKLVLSEVGHEAAAILTELNGPEAAFMDGAGGMSNQL
                     VLMHRGMSIAGGTSEIKRNQIGERILGLPRDPLLK"
     gene            complement(1659..2207)
                     /locus_tag="VLR27_11885"
     CDS             complement(1659..2207)
                     /locus_tag="VLR27_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04199.1"
                     /translation="MTSTITRDEDKNMISQLKALPREAVLGTLRLWRKPLGLAESVFA
                     KESADQAVSTWPPTVAYDRIASSVAHAAGVVLNDEVLLHTATVKKAKVTQLENAAKAE
                     AAAEAKRTEAEEELAEKRAEAQRKEREAAQRAEKRKAQARTEEQQAKQRVKKQAAAKE
                     KAAADAARKKQERIDAEAREAK"
     gene            complement(2204..2719)
                     /locus_tag="VLR27_11890"
     CDS             complement(2204..2719)
                     /locus_tag="VLR27_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04200.1"
                     /translation="MSDTTSDLPEGSAFDRFKAKAKQVAGDLLDRGDLAAEGTLEEAS
                     IDRADEAREQIAEAERAETEAELEARLAENEIEAERVEAEVEEQRRRDAVERDEARAE
                     AQVEAQADQREKAIVQQEAAERSQASREQVDALGERADAEEGVARLEAEAEAAKANAA
                     RLSALDAEEQS"
     gene            complement(2894..3073)
                     /locus_tag="VLR27_11895"
     CDS             complement(2894..3073)
                     /locus_tag="VLR27_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HSP04201.1"
                     /translation="MADAPKNKDDAKGRFKEAAGNLTGDEDLENEGKVDKAVGGVKDA
                     ADKAADKVKDVFRKD"
     gene            complement(3179..4381)
                     /locus_tag="VLR27_11900"
     CDS             complement(3179..4381)
                     /locus_tag="VLR27_11900"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HSP04202.1"
                     /translation="MSTRALVGSAVAVVGVAVLSVLAVGAVRASDAPSARCGDGAAAG
                     VRCPSDEAADEPTTSDVPGDRVATTTVPPAVAGSGDEVAVRLDLPPAPDPERPATLLP
                     LTEPSPSLPNPFVLVDGDRYLMFTTEARDAVGAKQNVPVLESPDLDTWRFVRDALPTV
                     GEWAVPEATWAPDVARIGDDWILFYTARVADLDPPTQCIGAAVGAAPEGPYQPQPEPI
                     VCQLDRGGSIDPRTYRDRDGTWWLHWKSDDNREVDGTATSTIHAQRLSATGVGLVGEP
                     AAVLEVTQPWEGRIVEAPHLIEAGGRLWLFYSANWFNQPAYGIGVAVCDTPVGPCAKP
                     FPGPWLSSNAQGEGPGEGSLFLDRRGRWRLVYSPVAQQYRAETDRPVALATVGFDDLG
                     PYLADPAG"
     gene            4469..5497
                     /locus_tag="VLR27_11905"
     CDS             4469..5497
                     /locus_tag="VLR27_11905"
                     /inference="COORDINATES: protein motif:HMM:NF022427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HSP04203.1"
                     /translation="MATHENELTEPVDLCTPDGERVNPSALGWSRRPLHRANLGGPFG
                     PNKRWDYWAVLAGDLLVSSVYADVDHLGLADVWWCDMASGATGGGGIVTVGSDDFDLP
                     ERPGTAPLHIDRDGVEIAYVDDGSGTRLTASWTEPDGRPGRLDVHVALPPGHESLNVV
                     IPWSDEVFNYTSKHQARPAAGELVVGDQRREIGGSAGDAWGVLDVGRGRWPAEIAWNW
                     GGGAGHVGEHVLGLQFGAKWTEGSGYTENGFILDGRLTKLGNELDWRYDWDAPMEPWH
                     IVDPGGQLDVTLHPRYDKHSQLPGRDKGSETHQVFGTFSGRVRTDDGVELEFDGLQGF
                     AEEARQEW"
     gene            5749..6666
                     /locus_tag="VLR27_11910"
     CDS             5749..6666
                     /locus_tag="VLR27_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04204.1"
                     /translation="MRKMLITLLAVMGLVLAGLTTANAAGPPEGHGPPTGGEEVGNNL
                     SVPAIFVDGGEQVFSLTCPSGPVPPVDDPAVTTDDPGTDFELEGFYFLQKVHRWQAQC
                     ATAEAVDVTVDWGDNLEGDALKAGTPIRVEVGLIDTDAVPMNGFVVEKLQPSLSDKAS
                     AYGTEATLVGDHYEATETQFPHTATVFNHEGLPEEVTVGIRVFDPDATFTLQNVDTGL
                     WVVGPDPVLMGSEMNAKGAIVYGYNWGIGNQGVKFLPTAGEHLLTFTAPNVDLTEGGE
                     VDHTATLGFFVQPNVKNNGKSAAAKANGR"
     gene            complement(6753..>7292)
                     /locus_tag="VLR27_11915"
     CDS             complement(6753..>7292)
                     /locus_tag="VLR27_11915"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HSP04205.1"
                     /translation="AELQSHFVVDVERSSEQALVGQLTQFTDIAVHAGSGSSGAPSVV
                     YETIWYLGAILHELVLHRLGRTERRTDDGRVLEWSPDPDAATLANVAIDRIRQVAAPE
                     PAMALELVRVLVDVRRAAARAGRDGLVDVLDHQADLIVEQCRRTDPLPTDLERVVAAR
                     HGGRHVDLMAERDEEYATA"
BASE COUNT         1036 a         2631 c         2579 g         1046 t
ORIGIN      
        1 gagcagttcg gccgtcccat cgccatgtac caggcggtga agcaccactg cgccaacatg
       61 gccgtggccc gtgagctcac caccagcgcc gtgtgggacg ccgcccgcgc cgccgccacc
      121 ggtggcgacc agctcagcta cgccgcggcc gtcgccgcca ccctggccgg ccccgccgcc
      181 gacctgtgcg ccaacctcaa cacgcaggtg cacggtggca tcgccatcac ctgggagcac
      241 gacgcccacc tctacatgcg ccgggccacc acgctcctca cgctgctcga gcccaagcag
      301 gccgccgccg aaatcgtcta cctcgtccgc gccggggtca cgcgggccaa ggccgtcgag
      361 ctgccgcccg aggccgaggc gatccgcgac gaggtccgca ccttcgccga ctccatcaag
      421 gacctgtcga aggacgagca gcgcacgaag ctgatcgaga ccggctacgt catgccccag
      481 tggcccgagc cctacggccg ggccgccggt gccatcgagc agctggtgat cgaggaggag
      541 ttcgccgccg ccggcatcca gcgccccggc tccggcatca ccgcctggaa catcctgacg
      601 atcatccagt acggcaccca ggaccagctc gaccgctggg tgaacccggc cctgcaccag
      661 gacgtcatct ggtgccagct gttctccgag cccgatgccg gctccgacgc cgccggcgtg
      721 aagaccaagg ccacccgggt cgacggcggc tggctggtca acggtcagaa ggtgtggacc
      781 tcgggcgccc acgtcgccgg catgggcttc gccaccgtgc gcaccaaccc cgacgtcccc
      841 aagcacgacg gcatcacgat gatggccatc gacatgcacg ccgagggcgt cgaggtccgg
      901 ccgctgaaga tgaccaacgg cggctcggag ttcaacgagg tgttcttcaa cgacgtcttc
      961 gtccccgacg acgacgtggt cggccccgtc gacggcggct ggaccgtcgc ccgctccacc
     1021 ctcggcaacg agagcgtgag catcggcggc ggtggcggcg gcatgtcgat gccgggcgcc
     1081 gcgctcgtgc cgccgctcga cgccaacccc gatcgcctca ctggcggcgc ggctcgcgtc
     1141 gggcgctaca tcgcccagca ccaggccatg ggcctgctca acctgcgggc ggccaaccgc
     1201 gccgtcgccg gcggcgagcc cggccccgag ggcgccatga ccaagctggt cctgtccgag
     1261 gtcgggcacg aggccgccgc catcctcacc gagctcaacg gccccgaggc cgcgttcatg
     1321 gacggcgccg gcggcatgag caaccagctc gtgctgatgc accgcggcat gtcgatcgcc
     1381 ggcggcacgt cggagatcaa gcgcaaccag atcggcgagc ggatcctcgg cctgccccgg
     1441 gacccgctgc tcaagtagcg aaaagcggtt ctggcctaca tcggtgacca ccaggtggca
     1501 cggatgtagg ccagaacgac gccggcgtca ggtcagcggg cggacttgcg ggccttcttg
     1561 accgcttcgg ccgccttctt ggtggtcgcg gcggccttgg tggcctcgag cgcctcgcgc
     1621 tgctcgccga gggcttcctt ctcggcctcg agctcgcgct acttggcctc gcgggcctcg
     1681 gcgtcgatcc gctcctgctt cttgcgcgcc gcgtcagcgg cggccttctc cttggccgcg
     1741 gcctgcttct tcacgcgctg cttggcctgc tgctcctcgg tgcgggcctg cgccttgcgc
     1801 ttctcggctc gctgggcggc ctcgcgctcc ttgcgctggg cctcggcccg cttctcggcc
     1861 agctcctcct cggcctcggt ccgcttggcc tcagcggcgg cttcggcctt cgcagcgttc
     1921 tcgagctggg tgaccttggc cttcttcacg gtggcggtgt gcagcaggac ctcgtcgttc
     1981 aggacgacgc cggcggcgtg ggccacggag gatgcgatcc ggtcgtaggc gaccgtgggc
     2041 ggccaggtgg agacggcctg gtcggccgac tccttggcga acacggactc ggcgagaccg
     2101 aggggcttgc gccacagtcg cagcgtgccg agcacggcct cgcggggcag ggccttgagc
     2161 tgggagatca tgttcttgtc ctcgtctcgg gtgatggtgc tggtcacgac tgctcctcgg
     2221 cgtcgagggc gctgagccgg gcggcgttgg ccttggcggc ctcggcctcc gcctcgaggc
     2281 gggcaacgcc ctcttcggcg tcggcccgct cgccgagcgc gtcgacctgc tcgcgcgacg
     2341 cctggctgcg ctcggcggcc tcctgctgga cgatcgcctt ctcgcgctgg tcggcctggg
     2401 cctcgacctg ggcctcggcc cgggcctcgt cccgctcgac ggcgtcccgg cggcgctgct
     2461 cctccacctc ggcctcgact cgctcggcct cgatctcgtt ctcggccagg cgagcttcga
     2521 gctcggcctc ggtctcggcg cgctcggcct cggcgatctg ctcgcgggcc tcgtcggccc
     2581 gatcgatgct ggcctcctcg agggtgccct cggctgcgag gtcgccgcga tcgaggagat
     2641 cgccggccac ctgcttggcc ttggccttga atcggtcgaa ggcgctgcct tcggggagat
     2701 cgctcgtggt gtcggacatg gttgcgtgct cctgggtggg tcgggggtgc ggttacccgc
     2761 gggtacgtcg ttacccgttc gcaacacttc cggtcacccc acgacccgga aatgcgtcac
     2821 aggggacgtc gggccggacg cgccacggcc ccgggcgaac ccggggccgt gtggtgcgca
     2881 gatcgaggct cgttcagtcc ttgcggaaga cgtccttgac cttgtcggcg gccttgtcgg
     2941 ctgcgtcctt gacgccgccg accgccttgt cgaccttgcc ctcgttctcg aggtcctcgt
     3001 cgccggtgag gttgccggcc gcttccttga agcggccctt cgcgtcgtcc ttgttcttcg
     3061 gtgcgtcagc catggtggtt ccttcgggtc ggggtggtgg gctggcgcat cccttccccc
     3121 cgccgatgcc gacgagacat cgaccgaagg cgagcagtac cggtgcgacg accgatgatc
     3181 agcccgccgg gtccgccagg tacgggccga ggtcgtcgaa gccgaccgtg gcgagagcga
     3241 ccgggcggtc ggtctcggcg cggtactgct gggccaccgg gctgtagacg aggcgccagc
     3301 ggccccggcg gtcgaggaac agcgatccct cccccggtcc ctcaccctgg gcgttggacg
     3361 acagccacgg cccagggaac ggcttggcgc acggacccac cggcgtgtcg cagacggcca
     3421 cgccgatgcc gtaggccggt tggttgaacc agttggccga gtagaagagc cacagccgac
     3481 cgccggcctc gatcaggtgc ggggcctcga cgatgcgacc ctcccagggc tgggtcacct
     3541 cgagcacggc ggccggctcg ccgaccaggc cgaccccggt ggcggacagg cgctgggcgt
     3601 ggatcgtcga ggtggcggtg ccgtcgacct cgcggttgtc atcggacttc cagtgcagcc
     3661 accacgtgcc gtcccggtcg cggtacgtgc gcgggtcgat gctgccgccg cgatccagct
     3721 ggcacacgat cggctccggc tgcggttggt aggggccctc cggcgccgca cccaccgccg
     3781 cgccgatgca ctgcgtcggc ggatccaggt cggccacgcg tgcggtgtag aagaggatcc
     3841 agtcgtcacc gatgcgggcc acgtcgggag cccacgtcgc ctcggggacc gcccattcgc
     3901 cgacggtggg cagtgcgtcg cgcacgaagc gccacgtgtc caggtccggc gactcgagca
     3961 cgggcacgtt ctgcttggct ccgaccgcgt cgcgtgcctc ggtggtgaac atcaggtacc
     4021 ggtcgccgtc gacgaggacg aaggggttcg gcagagacgg cgacggctcg gtcagcggca
     4081 gcagggtcgc cggccgctcc gggtccggcg ccggcggcag gtcgaggcgg acggcgacct
     4141 cgtcgccgga gccggcgacg gcgggaggga cggtcgtcgt cgccacccgg tcaccgggaa
     4201 cgtccgacgt cgtcggctcg tccgcagcct cgtccgaggg acagcgaact cccgcggcgg
     4261 ccccgtcccc gcaccgtgcc gacggtgcgt cgctggcccg gacggcaccg acggccagga
     4321 cggagaggac ggcgaccccg acgacggcga cggcggatcc gacgagggct cgggtggaca
     4381 tcgtggtgcg tggcctcggg acgtcacccg ggcaggacgc cgggtgggtc cgctgaggct
     4441 accgacgcga cttcggtagg aaggagcagt ggcgacccat gagaacgagc tgaccgaacc
     4501 cgtcgacctg tgcacgccgg acggcgagcg cgtgaacccg agcgcgctgg gctggtcacg
     4561 ccgaccgctg caccgagcga acctcggtgg ccccttcggc ccgaacaagc ggtgggacta
     4621 ctgggcggtg ctcgccgggg acctgctcgt gtcctcggtg tacgccgacg tcgaccacct
     4681 cggcctcgcc gacgtgtggt ggtgcgacat ggcctccggc gccaccggcg ggggcggcat
     4741 cgtcaccgtc ggcagcgacg acttcgacct ccccgagcgg cccggcacgg caccgctgca
     4801 catcgaccgc gacggcgtcg agatcgccta cgtcgacgac ggatccggca cccggctcac
     4861 cgcgtcctgg accgagcccg acggccgtcc gggccggctc gacgtgcacg tcgcgctgcc
     4921 gcccggtcac gagtcgctga acgtcgtcat cccgtggagc gacgaggtct tcaactacac
     4981 ctccaagcac caggcccgcc cggccgccgg cgagctggtc gtcggcgacc agcgtcggga
     5041 gatcggcggc agcgccggcg acgcgtgggg cgtgctcgac gtgggacgcg ggcggtggcc
     5101 cgcggagatc gcctggaact ggggcggtgg tgccggacac gtcggcgagc acgtgctcgg
     5161 cctgcagttc ggagcgaagt ggaccgaggg atcgggctac accgagaacg ggttcatcct
     5221 cgacggccgc ctcaccaagc tcggcaacga gctcgactgg cgctacgact gggacgcgcc
     5281 gatggagccg tggcacatcg tcgacccggg cggccagctc gacgtcaccc tccacccccg
     5341 ctacgacaag cacagccagc tccccgggcg ggacaagggc tccgagaccc accaggtgtt
     5401 cggcacgttc tccggccggg tccgcaccga tgacggcgtc gagctcgagt tcgacgggct
     5461 gcagggcttc gccgaggagg cccgccagga gtggtgacct gagcgacgcg ggcagccgtg
     5521 ttgcacggag cgatgcccgc cggccacgca tcgagtgccg cgcgccctac caggccggca
     5581 tgggcaggcc cacggtcgaa cgacccgagt ccgcagggcc cttcgtacct acccaccggt
     5641 aacaacgcag ccgacgatgg cggtgcaccc atccaagcgg aggtgagcca atgaagaccc
     5701 ggcacagcag aggctccctg accacccaca tcggatagag gagagatcat gaggaagatg
     5761 ctcatcaccc tgctggcagt gatgggcctg gtgctcgccg gactcaccac agccaacgca
     5821 gccggccccc cggaggggca cgggccgccc acgggcggcg aggaggtggg gaacaacttg
     5881 tcggtgccgg cgatcttcgt cgacggcggc gagcaggtgt tctcgctgac gtgcccgagc
     5941 gggccggtcc cgccggtgga cgaccccgcc gtgaccacgg acgacccagg caccgacttc
     6001 gagctggagg ggttctactt cctccagaag gtgcaccgtt ggcaggccca gtgcgccacg
     6061 gcagaagcag tggacgtgac cgtggactgg ggtgacaacc tcgagggcga cgccctcaag
     6121 gccgggaccc cgatccgtgt cgaggtcgga ctcatcgaca ccgacgccgt tcccatgaac
     6181 gggttcgtcg tcgagaagct gcagcccagc ctcagcgaca aggcatccgc ctacggcacc
     6241 gaggcaacgt tggtcggtga ccactacgag gcaacggaga cccagttccc gcacaccgcg
     6301 acggtcttca accacgaggg cctgccggag gaggtgacgg tcgggatccg ggtgttcgac
     6361 cccgacgcaa cgttcaccct gcagaacgtc gacaccgggc tgtgggtcgt cggacccgat
     6421 ccggtgctga tgggctcgga gatgaacgcc aagggcgcca tcgtctacgg ctacaactgg
     6481 ggcatcggca accagggcgt caagttcctg ccgacggcgg gtgagcacct gctgaccttc
     6541 acggccccga acgtggacct gaccgagggc ggcgaggtcg accacaccgc gacgctcggg
     6601 ttcttcgtcc aacccaacgt gaagaacaac ggcaagagcg ccgccgccaa ggcaaacggc
     6661 cggtagcgcc ggctccaccg aggagtcccg ggtcgcagga cccggggctc cttccgcgtc
     6721 tggtcagcac gaagccgctg tgcgcctccg cctcaggccg tggcgtactc ctcgtcccgt
     6781 tccgccatga ggtcgacgtg gcggccgccg tggcgggcgg cgacgacccg ctcgaggtcg
     6841 gtcgggagcg gatcggtccg gcggcactgc tcgacgatca ggtcggcctg gtgatccagc
     6901 acgtcgacca gaccgtcacg accggcccgg gcggcggcgc ggcgcacatc gaccagcacg
     6961 cggaccagct cgagggccat ggccggctcc ggcgcagcga cctggcggat gcggtcgatc
     7021 gccacgttgg cgagcgtcgc cgcgtcgggg tccggtgacc actcgagcac ccggccgtcg
     7081 tcggtgcggc gctcggtccg gccgagccgg tgcagcacca gctcgtggag gatcgcgccg
     7141 aggtaccaga tcgtctcgta gacgaccgac ggcgctcccg agctcccgga gccggcgtgg
     7201 accgcgatgt cggtgaactg ggtgagctgc ccgacgagcg cctgctccga cgagcgctcc
     7261 acgtccacga cgaagtgcga ctgcagctcg gc
//
LOCUS       DATISU010000296         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_578476, whole genome shotgun sequence.
ACCESSION   DATISU010000296 DATISU010000000
VERSION     DATISU010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578476"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..993)
                     /locus_tag="VLR27_11920"
     CDS             complement(4..993)
                     /locus_tag="VLR27_11920"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HSP04206.1"
                     /translation="MHVSELPTPALVVERAVFDRNVARMAERHPGPRLRPHVKAFKST
                     ALARELATAGHTGFCCATVAEVLGMARAGLGDDLLLANETLDPRRLRAMAELDVPVTV
                     AVDSDQTVDAAAAARIQRVLVDVEVGLPRCGCDADDAGRLADRARAAGMEVRGVMGYE
                     GHVNVLLDREERRAAVEKAMTRLLRAHDLVGGDVVSGGGTMTAELNEWVTEVQAGTYV
                     LMDTSFAPQVPFEIAIGVEATVISVSPKGWAVADAGLKAFGMDSGKPSLPGADVWIVS
                     DEHVTLTGLDVSVGDRVRLLPAHLDPTVAKHERMWVLDGDEVVDEWPVDLRGW"
     gene            complement(1013..1798)
                     /locus_tag="VLR27_11925"
     CDS             complement(1013..1798)
                     /locus_tag="VLR27_11925"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HSP04207.1"
                     /translation="MPEGHTIHRLARDLAEDLLDRPVDATTRQERFADGARRLGGQVL
                     TGTEAHGKHLFLHWDGGEVLYVHLGLIGKFQRHEVPAPDPSPNLRLRLEGEATAWDLT
                     GPMTCELREPDVVDQVTATLGPDPLRPRADPQPFLDAVRRSRKPIGALLLDQSVVAGI
                     GNVYRAEILHLAGIDPSTPGQALSDDEARTLWRLAVEQLRLGLKRNRIVTVPLGGRRL
                     TSISREESFHVYKQERCRTCGADVVRVPIGNRTSYACATCQPA"
     gene            1834..1917
                     /locus_tag="VLR27_11930"
     tRNA            1834..1917
                     /locus_tag="VLR27_11930"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1868..1870,aa:Leu,seq:tag)
     gene            2002..2568
                     /locus_tag="VLR27_11935"
     CDS             2002..2568
                     /locus_tag="VLR27_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HSP04208.1"
                     /translation="MPGLDAQGFQIYNQLLSNRIVFLGTDVNDQIANQICAQLLYLEG
                     QDAEKDIWLSINSPGGSVTAGMAIYDTMQFVSCDVATVCLGLAASMGQFLLTSGAANK
                     RFTLPNARIMMHQPLAGLRGQATDIAIQAEQLRYTKQRMAQLISEHSGQPIEKIQADS
                     ERDRWFTAEQAKEYGLVDKVILRRGEIV"
     gene            complement(2603..3079)
                     /locus_tag="VLR27_11940"
     CDS             complement(2603..3079)
                     /locus_tag="VLR27_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04209.1"
                     /translation="MLPRQFVIAVALAASATALAGCGDDEVASDAERFCGEATVNRDM
                     IVAPPLSNEAEVAATLDFYRLMGQLAPIAIAEEWNELVVAMETAAAIVPGDPASEQEV
                     AMTAYATERSAYEIAVWLQRNCGVAIPITTIAPQAPLPPPTTALVPPPSTVATVTE"
BASE COUNT          476 a         1168 c         1195 g          469 t
ORIGIN      
        1 cgctcaccac ccgcgcaggt ccaccggcca ctcgtcgacg acctcgtcgc cgtcgagcac
       61 ccacatgcgc tcgtgcttgg cgacggtggg atcgaggtgg gcggggagga gccggacccg
      121 gtcgcccacc gagacgtcga gcccggtgag ggtgacgtgc tcgtccgaca cgatccagac
      181 gtccgcaccc ggcagtgagg gcttgccgct gtccatcccg aacgccttca gcccggcgtc
      241 ggccacggcc caccccttgg ggctgacgga gatgaccgtc gcctccaccc cgatcgcgat
      301 ctcgaagggc acctgcgggg cgaacgaggt gtccatcagc acgtaggtgc cggcctgcac
      361 ctcggtgacc cactcgttga gctcggcggt catcgtcccg cctccggaga cgacgtcgcc
      421 gccgacgagg tcgtgggccc ggagcagccg ggtcatggcc ttctccaccg cggcgcgtcg
      481 ctcctcccgg tcgagcagga cgttgacgtg gccctcgtag cccatgaccc cgcgcacctc
      541 catgccggcg gcgcgagccc gatcggccaa ccggccggca tcgtcggcat cgcacccgca
      601 gcgggggagg ccgacctcga cgtcgacgag cacccgctgg atcctcgccg cagcagcggc
      661 atcgaccgtc tggtcggagt cgaccgccac cgtcaccggc acgtcgagct cggccatcgc
      721 ccggaggcgt cgggggtcga gcgtctcgtt ggccaggagc aggtcgtcgc ccaggccggc
      781 gcgtgccatg cccaggacct cggccaccgt cgcgcagcag aagcccgtgt gaccggcggt
      841 cgcgagctcc cgggccagcg cggtcgactt gaacgccttg acgtgcggcc ggagccgcgg
      901 tcctgggtgg cgctcggcca tgcgggcgac gttccggtcg aacaccgcac gttcgacgac
      961 gagggcgggc gtcgggagct ccgagacgtg cacgctcgga cgctacgccc ggtcaggccg
     1021 gctggcaggt ggcgcaggcg taggaggtgc ggttcccgat ggggacgcgc accacgtcgg
     1081 cgccgcacgt ccggcaccgc tcctgcttgt agacgtggaa cgactcctcg cgggagatgc
     1141 tggtcagtcg ccgcccaccg agcgggacgg tgacgatccg gttgcgcttc agcccgaggc
     1201 ggagctgctc gacggccagg cgccagaggg tgcgagcctc gtcgtccgag agcgcctggc
     1261 cgggggtgct ggggtcgatg ccggccaggt gcaggatctc ggcgcggtag acgttgccga
     1321 tgccggcgac caccgactgg tcgaggagca gggcgccgat gggcttgcgg ctccgccgga
     1381 cggcgtcgag gaacggctgg gggtcggccc gtggtcgcag cgggtcgggg ccgagcgtgg
     1441 ccgtcacctg gtcgacgacg tcgggttccc gcagctcaca ggtcatgggg ccggtgaggt
     1501 cccacgccgt cgcctcgccc tccaggcgca ggcggaggtt cggggaggga tccggtgcgg
     1561 ggacctcgtg gcgctggaac ttcccgatga ggccgaggtg gacgtagagc acctcgccac
     1621 cgtcccagtg caggaagagg tgcttgccgt gggcctcggt gccggtgagc acctggccgc
     1681 cgaggcggcg tgcgccgtcg gcgaagcgct cctggcgggt ggtggcgtcc accgggcggt
     1741 cgagcaggtc ctcggccagg tcccgggcga ggcggtggat ggtgtggccc tcgggcatcg
     1801 ggccaggatg cctgggtacg ttgtctgccc cacgcatcag tggtggaact ggcagacacg
     1861 cagggtttag gtccctgtgc gcgagagcgt gtgtgggttc gagtcccacc tgatgcaccc
     1921 tcggcccgcg cggcgacccg tcgccccgcc gctagattcc gacccgtgac tgatcagctc
     1981 ccgaccccac tcgccgccga tctgccgggg ctcgacgcgc agggcttcca gatctacaac
     2041 cagctgctgt cgaaccgcat cgtcttcctc gggaccgacg tcaacgacca gatcgccaac
     2101 cagatctgcg cccagctcct gtacctcgag ggccaggacg ccgagaagga catctggctg
     2161 tccatcaaca gcccgggcgg ctccgtcacc gccggcatgg cgatctacga cacgatgcag
     2221 ttcgtctcct gcgacgtcgc cacggtgtgc ctcggcctcg ctgcgtcgat ggggcagttc
     2281 ctcctcacgt ccggagctgc aaacaagcgg ttcacgctgc ccaacgcccg gatcatgatg
     2341 caccagccgc tcgctggcct ccgcggtcag gccaccgaca tcgcgatcca ggccgagcag
     2401 cttcgataca ccaagcagcg catggcgcag ctcatctccg agcactccgg tcagccgatc
     2461 gagaagatcc aggccgacag cgagcgcgac cgctggttca ccgccgagca ggccaaggag
     2521 tacggcctgg tcgacaaggt gatcctgcgc cgcggcgaga tcgtctgacc cgacgcgtcc
     2581 tcgcgcctcg ggcgccgtca ggtcactcgg tgacggtggc gaccgtcgag ggcggcggca
     2641 cgagtgccgt cgtcggtggc gggagcggtg cctgcggggc gatcgtggtg atcgggatgg
     2701 cgacaccgca gttgcgctgc agccagaccg ctatctcgta ggccgatcgc tcggtcgcgt
     2761 aggcggtcat ggcgacctcc tgctccgacg ccgggtcgcc cggcacgatc gccgccgccg
     2821 tctccatcgc cacgacgagc tcgttccact cctcggcgat cgcgatcggg gcgagctgcc
     2881 ccatcaagcg gtagaagtcg agtgtggcgg cgacctcggc ctcgttcgac aacgggggag
     2941 cgacgatcat gtcgcggttg acggtggcct cgccgcagaa ccgttcggcg tcgctcgcca
     3001 cctcgtcgtc gccgcaccct gcgagggcgg tggccgacgc ggcgagcgcg acggcgatga
     3061 cgaactggcg ggggagcacg ccacgaagtg tggcacggcg cgatgccggc gcccgggcaa
     3121 ccccgtcgtg tagccgccgt caaccgcctg ctgcgcgagg gcgcgccacc cgccacgcca
     3181 gccacagcag tggcagctgg aacggtaggc ggagccacgc agcggcggcg gagtcgaacg
     3241 gcggatccat cccgtccata ccgccgtcga gggcagcctg gatgttcgcc gggtagaccg
     3301 cgagcagg
//
LOCUS       DATISU010000297         5897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_206028, whole genome shotgun sequence.
ACCESSION   DATISU010000297 DATISU010000000
VERSION     DATISU010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206028"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..856)
                     /locus_tag="VLR27_11945"
     CDS             complement(29..856)
                     /locus_tag="VLR27_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HSP04210.1"
                     /translation="MLKALRRWWKYLVARTDSSLNERADPKVQLEQAISEAHEQHKRL
                     TQQAANVIANQKQTEMRLNRVMTELEKVSGNARQAVLMADQAQKSGDTAKATEYTQAA
                     ESFANRMITLESEVEDLKALHFQASEASDQAKVAVQQNATALQKKLSERQALLGKIDQ
                     ANMQEEMNKAMSQLSMTVGEDVPSLEEVRSKVEARYAKAKGMQELQGSSVEGRMLEVE
                     QAALNVEATSRLAEIKAQLGIAPDAAEAEPEAIAGESAPEGQTQAEGQTQPSDGTAS"
     gene            complement(914..2623)
                     /gene="murJ"
                     /locus_tag="VLR27_11950"
     CDS             complement(914..2623)
                     /gene="murJ"
                     /locus_tag="VLR27_11950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HSP04211.1"
                     /translation="MSEPRTGSRAGQRAARLRQQANDPLPEAEARPDASPRRPPATDD
                     SGRNASVVGAGILLSRLSGLVREMVTARFLGVGLASDAFKAALRIPNLLQNLLGEGVL
                     SASFIPVYARLVDTDEEEAGRTAGAVAGLLAAAVSVLVVVGMVAARPITIVLTPGFRD
                     DPEKLDLTVSLVRIMFPGIGLLVLSAWCLAILNSHRRFFLSYVAPVLWNVAQIVVVAV
                     VAIGGSTPISLANALAWGVVLGGLAQLLVQLPTVRTLVPDLRLSLDRTLTGVRDAMSR
                     LGPVVFGRGVVQISGYLDLVLASLLVTGAVTAGTYAQVLYLLPVSLFGMSIAASELPE
                     LSRMRPEQRAELAGRVEGGLRRVAYFVAPTAVIYIALGEPLVAAVFQGGEFSVSDTRL
                     VWLVLAAYSLGLLASTGSRLLQNSLYALDDPRTPARIALVRVVTSSLIALSVMFLFDR
                     LVLVGTSIQLETDATDTAAFLHLGAVGIALGSAVGAWVELALLRLALRWRIGPVRLGG
                     GSLATVVTAALAGAAVGRLVDLVVGDLPAILAAGASIGAAGLVYLAFAQARGLPVRAM
                     LGR"
     gene            complement(2620..4026)
                     /locus_tag="VLR27_11955"
     CDS             complement(2620..4026)
                     /locus_tag="VLR27_11955"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSP04212.1"
                     /translation="MLPDPALDDAVRAFCDALTPRVRDLAATVKGVDGERIEVDVQVE
                     AYNLAAAFIDADGLHTDPELLAFIAAFGPRFGGSLARSAPADVRSARLLADRVRFLDQ
                     PSELAEVLLAADGRDGTDLASLYYDRALAIGFAVASLDQHTAPVELAAIERFRGRLLD
                     RIAETRGVGRREAAHTAATVGDGAEPVPAQPDLPPARPVDEVLAELDELIGLEAVKAE
                     VKKVADLTRVQLLRAERGLPVLDQSRHLVFSGNPGTGKTTVARLLAEIYRSLGVVEHG
                     HLVEVDRAGLVAGFVGQTAPKVTAVFDEADEGVLLIDEAYALVRGSETDFGREAIDTI
                     VKLVEDRRDRIVVIMAGYPDEMSELVAANPGLRSRFPKTIHFPDYDTDELLAIFDMRC
                     RRGGYDTTHEARAKVRSHLDAAPRDKGFGNGRVARNLFEEAVARQASRIVTIEDPTDD
                     QLRTLEADDIPDHRATEA"
     gene            4072..5319
                     /locus_tag="VLR27_11960"
     CDS             4072..5319
                     /locus_tag="VLR27_11960"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HSP04213.1"
                     /translation="MADSPMAQLGARAKAASRVLATASTAAKDAALLAAADLLVARSE
                     AILEANERDVAREEAGGMAPALLDRLRLSSARIEAMAGGLRQVAALPDPVGEISEGWT
                     RPNGLRINRVRVPLGVVAIIYESRPNVTSDAFGLCLKSGNAAFLRGSSAAIESNTAIA
                     AALREGIDKAGLPADALVLVEDVSREAAVEFMQLRGMVDCLIPRGGRSLIQSILDNAT
                     VPFVIDGDGNCHVYVDAAADLSVALDIIVNAKTQRPGVCNAAEKLLVHADVAADFLPR
                     AAAALEGVELVGDEAACGILTSASPATDEDWDTEFLDLKLGVAVVDSIDAAIDHIARH
                     GSGHSEAIVTTDLTAADRFTTEVDAAAVVVNASTRFVDGEEFGFGAEIGISTQKLHAR
                     GPMGLRQLTTEKFVVRGTGQTRS"
     gene            5407..5748
                     /locus_tag="VLR27_11965"
     CDS             5407..5748
                     /locus_tag="VLR27_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04214.1"
                     /translation="MSDTDQRVVFLERNAAEELLDLVDDRPGDADEDLLDAALDAIET
                     AGDGLPGEAQQAVARGDAVALGLDPTEADTVRTLILENHEADPGVLRSVGASIKSKLK
                     AAAKKARRKKR"
BASE COUNT          858 a         2094 c         2137 g          808 t
ORIGIN      
        1 gcttcgtgtg acgaggcggc tgccagtttc aagaagcggt gccgtccgag ggttgagtct
       61 ggccctcggc ctgggtctga ccctcgggag cggactcgcc ggcgatggcc tcgggctccg
      121 cctcagcggc gtccggagcg atgccgagct gcgccttgat ctcggccagc cggctggtgg
      181 cctccacgtt cagcgcggcc tgctcgacct cgagcatgcg accctcgacc gaggagccct
      241 gcagctcctg catccccttg gccttggcgt agcgggcctc gaccttggag cggacctcct
      301 cgagggaggg gacgtcctcg ccgaccgtca tggagagctg gctcatcgcc ttgttcatct
      361 cctcctgcat gttggcctgg tcgatcttgc cgaggagggc ctggcgctcg gagagcttct
      421 tctgcagggc ggtggcgttc tgctgcacgg cgaccttggc ctggtcggat gcctcggacg
      481 cctggaagtg cagggccttg aggtcctcga cctcgctctc cagcgtgatc atccggttgg
      541 cgaaggactc ggcggcctgg gtgtactcgg tggccttggc ggtgtcaccg gacttctggg
      601 cctggtcggc catcagcacg gcctggcggg cgttgcccga gaccttctcc agctcggtca
      661 tcacccggtt gaggcgcatc tcggtctgct tctggttggc gatgacgttc gccgcctgct
      721 gggtgaggcg cttgtgctgc tcgtgcgcct cggagatcgc ctgctcgagc tgcaccttgg
      781 ggtcggctcg ctcgttgagc gagctgtccg tccgggcaac caagtacttc caccaacgtc
      841 tgagagcctt gagcatggtc ggatcgtaga cccctccgcc cccgtcgccg cctcgttcct
      901 gcgtccgggg cggtcagcgg cccagcatcg cccgcaccgg caggccacgg gcctgggcga
      961 acgccaggta cacgaggccg gcggcgccga tggatgcacc cgctgccagg atcgccggca
     1021 ggtccccgac gacgagatcg acgagccgac cgaccgcagc gccggcgagg gcggcggtga
     1081 ccacggtggc gagcgagccg ccaccgaggc gcaccggccc gatgcgccag cggagcgcga
     1141 ggcgcagcag ggcgagctcc acccaggccc cgaccgccga gccgagggcg atgccgacgg
     1201 cgccgaggtg caggaaggcg gcggtgtcgg tggcgtcggt ctcgagctgg atcgaggtgc
     1261 ccaccaggac gagccggtcg aagaggaaca tcaccgacag cgcgatcagc gaggaggtga
     1321 ccacccggac cagggcgatg cgggccggtg tgcgggggtc gtccagggcg tagagcgagt
     1381 tctgcaggag gcgggagccg gtggaggcga gcaggcccag ggagtaggcg gccagaacca
     1441 gccacaccag tcgcgtgtcc gagacgctga actccccacc ctggaacacc gcggccacga
     1501 gcggctcgcc gagcgcgatg tagatcaccg ccgtcggggc cacgaagtac gccacccgcc
     1561 tcaggccgcc ctcgacccgg ccggccagct cggctcgctg ctcgggccgc attcgggaca
     1621 gctccggcag ctccgatgca gcgatgctca tgccgaagag cgagacgggc aggaggtaca
     1681 ggacctgggc gtaggtgccc gccgtgactg cgccggtgac gaggaggctg gccaggacca
     1741 ggtcgaggta gcccgagatc tgcaccacgc cccggccgaa caccaccggg ccgagccggc
     1801 tcatggcatc gcggacgccg gtgagggtgc ggtcgaggga caggcgcagg tcggggacga
     1861 gcgtgcggac ggtggggagc tggacgagca gctgggccag cccgccgagc accacacccc
     1921 aggccagggc gttggccagg ctgatggggg tcgacccgcc gatggcgacg acggccacca
     1981 ccacgatctg ggcgacgttc cacaggaccg gggcgacgta ggacaggaag aagcggcggt
     2041 gcgagttcag gatcgccagg caccacgccg acaggacgag cagcccgatg cccgggaaca
     2101 tgatccggac caggctgacg gtgaggtcca gcttctcggg gtcgtcgcga aagcccgggg
     2161 tgaggacgat ggtgatgggc cgggccgcca ccatgccgac aaccacgagg accgacaccg
     2221 cagcggcgag caacccggcg accgcaccgg cggtgcggcc ggcctcctcc tcgtcggtgt
     2281 cgacgaggcg tgcgtacacg gggatgaagg aggcggacag gaccccctcg cccaggaggt
     2341 tctggagcag gttggggatg cgcagggccg ccttgaacgc gtcggaggcc agcccgacgc
     2401 cgaggaaccg ggcagtgacc atctctcgga ccaggccgga gaggcgggac agcaggatgc
     2461 cggcgccgac gacgctggcg ttgcgcccgg agtcgtcggt ggccggcggg cggcgcggcg
     2521 acgcgtcggg gcgggcctct gcctccggaa gcgggtcgtt cgcctgctgt cgcagccgcg
     2581 ccgcccgctg gccggcgcgg ctgcccgtgc ggggttcgct cacgcctcgg tcgcccggtg
     2641 gtcggggatg tcgtcggcct cgagcgtgcg gagctggtcg tccgtcgggt cctcgatggt
     2701 cacgatccgg gacgcctggc gggcgacggc ctcctcgaac aggttccggg cgacccggcc
     2761 gttgccgaag cccttgtccc gcggtgcggc atcgaggtgc gaccgcacct tcgcccgcgc
     2821 ctcgtgggtg gtgtcgtagc caccccggcg gcagcgcatg tcgaagatgg ccagcagctc
     2881 gtcggtgtcg tagtcgggga agtggatggt cttggggaag cgggatcgca ggcccgggtt
     2941 cgccgccacc agctcggaca tctcgtcggg gtacccggcc atgatcacca cgatccggtc
     3001 ccggcggtcc tccaccagct tcacgatcgt gtcgatggcc tcccgcccga agtcggtctc
     3061 gctgccccgg accagggcgt aggcctcgtc gatgagcagc acgccctcgt cggcctcgtc
     3121 gaacaccgcg gtgaccttcg gcgcggtctg ccccacgaac ccggccacga gtccggcccg
     3181 gtcgacctcc accaggtgcc cgtgctcgac cacgccgagg gaccggtaga tctcggcgag
     3241 gagccgggcg acggtggtct tgccggtgcc ggggttgccg gagaacacca ggtgccgcga
     3301 ctggtcgagg accgggaggc cacgctcggc tcggagcagc tggacccggg tgaggtcggc
     3361 gaccttcttc acctcggcct tgaccgcctc gaggccgatc agctcgtcca gctcggcgag
     3421 cacctcgtcg acggggcggg ccgggggcag gtccggctgg gccgggaccg gctcggcgcc
     3481 atcgccgacc gtggccgcgg tgtgggccgc ctcacggcga ccgaccccgc gggtctcggc
     3541 gatccggtcg agcagccgcc cacggaagcg ctcgatcgcc gccagctcca ccggcgcggt
     3601 gtgctggtcg aggctggcca cggcgaagcc gatggcgagc gcgcggtcgt agtagaggct
     3661 ggcgaggtcg gtgccgtcgc ggccgtcggc ggcgaggagg acctcggcca gctcggaggg
     3721 ctggtcgagg aagcgcaccc ggtcggccag caggcgggcc gaccggacgt cggccggggc
     3781 cgatcgggcc aggctcccgc cgaagcgggg tccgaacgcg gcgatgaagg ccaggagctc
     3841 gggatcggtg tgcaggccgt cggcgtcgat gaacgccgcg gcgaggttgt aggcctccac
     3901 ctggacatcg acctcgatgc gctcaccgtc gacgcccttc accgtcgccg ccagatcccg
     3961 gacgcgcggg gtgagcgcgt cgcagaaggc acgcacggcg tcgtcgagcg cggggtccgg
     4021 cagcacgggc cgagtctgcc ccgtcgcggc atgcgtcctt agacttctcc catggctgac
     4081 tccccgatgg cccagctcgg cgctcgcgcc aaggctgcgt cccgcgtcct tgccaccgcg
     4141 tcgaccgccg cgaaggacgc ggccctcctc gccgcagccg acctgctcgt cgcccgcagc
     4201 gaggcgatcc tcgaggcgaa cgagcgcgac gtcgcacgcg aggaggccgg tggcatggcg
     4261 ccggcgctgc tcgaccggct ccgcctcagc tcggcccgca tcgaggccat ggccggcggc
     4321 ctgcgccagg tcgccgccct gcccgatccg gtcggtgaga tctccgaggg atggacccgc
     4381 ccgaacggcc tgcgcatcaa ccgggtccgg gtgccgctcg gcgtcgtggc catcatctac
     4441 gagtcccgcc ccaacgtcac cagcgacgcc ttcggcctgt gcctcaagtc tggcaacgcc
     4501 gccttcctgc gtggatcctc ggcggcgatc gagtcgaaca ccgccatcgc cgccgcgctg
     4561 cgcgagggga tcgacaaggc cggtctgccc gccgacgcac tcgtcctcgt cgaggacgtg
     4621 agccgtgagg ctgcggtcga gttcatgcag ctgcggggca tggtcgactg cctgatcccg
     4681 cgcggcggtc gctcgctcat ccagagcatc ctcgacaacg ccaccgtgcc cttcgtgatc
     4741 gacggcgacg gcaactgcca cgtctacgtg gacgcggccg ccgacctgtc ggtcgcgctc
     4801 gacatcatcg tcaacgccaa gacccagcgc cccggggtct gcaacgcggc cgagaagctc
     4861 ctcgtgcacg ccgacgtcgc cgccgacttc ctcccgcggg cggccgctgc gctggagggc
     4921 gtcgagctgg tcggcgacga ggccgcctgc ggcatcctga cctcggcctc gccggccacc
     4981 gacgaggact gggacaccga gttcctcgac ctcaagctcg gcgtcgccgt cgtcgactcc
     5041 atcgatgccg ccatcgacca catcgctcgc cacggctcgg gccactccga ggccatcgtc
     5101 accaccgacc tcaccgctgc cgatcgcttc accaccgagg tcgatgctgc ggcggtggtc
     5161 gtcaacgcgt ccacccgctt cgtcgacggc gaggagttcg gcttcggcgc cgagatcggc
     5221 atctccaccc agaagctcca cgctcggggt ccgatgggcc tgcgccagct caccacggag
     5281 aagttcgtcg tgcgcggcac cgggcagacc cgcagctgat cccgtctggc cgaccctccg
     5341 ggcgggtagg caccagagcg gagcagcggg cgcgacgggc gcacccgctg ggcaccgaaa
     5401 ggaaccatgt cagacaccga ccagcgcgtg gtgttcctcg agcgcaacgc cgccgaggag
     5461 ctcctcgatc tcgtggatga ccgcccgggc gacgccgacg aggacctcct cgacgccgcc
     5521 ctcgatgcga tcgagaccgc aggagacggc ctccccggcg aggctcagca ggccgtcgca
     5581 cgcggcgacg ccgtcgccct cggcctcgac ccgaccgagg ccgacaccgt gcggaccctc
     5641 atcctcgaga accacgaggc cgacccgggc gtgctgcgct cggtcggcgc cagcatcaag
     5701 tccaagctca aggctgcagc caagaaggca cgacgcaaga agcgctgacc gcaatagttg
     5761 accgagcggt caagacggcc ggtagggtcg ggagatggcc gaaccgcgct tcgattccgt
     5821 ccccgaggtc cgcgagggcc tgaagaaggt cgactacctc tccgacgagg gcatcgccgg
     5881 catcgtctac ctcgccg
//
LOCUS       DATISU010000298         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_66277, whole genome shotgun sequence.
ACCESSION   DATISU010000298 DATISU010000000
VERSION     DATISU010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66277"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VLR27_11970"
     CDS             <1..294
                     /locus_tag="VLR27_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HSP04215.1"
                     /translation="QTESQRGTIIPVEITIFEDRTFSFITKTPPTPVLIRQAAGLDKG
                     ATTTGTQVVGSITQAQLEEIAQTKMPDLNAYDLEAAKLQVAGTARSMGVEVKK"
     gene            383..1087
                     /gene="rplA"
                     /locus_tag="VLR27_11975"
     CDS             383..1087
                     /gene="rplA"
                     /locus_tag="VLR27_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HSP04216.1"
                     /translation="MAAGKRYRDATARYDRDANHAPAEALDLARSLANAKFDETIDLI
                     ARLGVDPRKADQIVRGTVALPSGTGTDVRVAVFAQGDAAQAARDAGAEHVGGDDLAEQ
                     VDGGMLDFDVAIATPDMMPTVGKLGRVLGPRGLMPNPKTGTVTTDVAKAVEEFKGGKV
                     EYRTDRYGNVHVPIGKSSFDSAQLAANFWAVVEELQRVRPASAKGRYFRRIGVSSTMG
                     PGIRIDTGRLKRETTD"
     gene            1283..2032
                     /gene="rplJ"
                     /locus_tag="VLR27_11980"
     CDS             1283..2032
                     /gene="rplJ"
                     /locus_tag="VLR27_11980"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HSP04217.1"
                     /translation="MENPRPEKVAVVDEVRERFDGTSAAILTEYRGLSVGQLADLRTR
                     LRGAGGDFKVYKNTLVRFAARDLDLEIEELLTGPTAIAFVTERPDGEPGDAVDVAKVL
                     KDFGKEFPALVVKGGLLGDKLLTADDTKALADVEPREVLLAKLAGAMAAPMQQLAGLL
                     QALPRNMAYGLKALIDQGGAEGAPADAAPAADADESTTDESPTDEAPAPAEAENTTDD
                     APATAEDDTPEAEDTPAAEAAPAADTDTQES"
     gene            2036..>2338
                     /gene="rplL"
                     /locus_tag="VLR27_11985"
     CDS             2036..>2338
                     /gene="rplL"
                     /locus_tag="VLR27_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002854784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HSP04218.1"
                     /translation="MATKEEILDAIANMTVLELSELLSDFEEKFGVTAAAPVAVAAAG
                     GPAAGAGEAEEEQDEFDVILKGAGDKKIGVIKEVRALTNLGLKEAKELVDGAPSPVL"
BASE COUNT          423 a          903 c          744 g          268 t
ORIGIN      
        1 cagaccgaga gccagcgggg cacgatcatc ccggtcgaga tcacgatctt cgaggaccgc
       61 acgttctcgt tcatcaccaa gaccccgccc acgccggtgc tgatccgcca ggccgcgggg
      121 ctcgacaagg gcgccaccac caccggcacc caggtcgtcg gctcgatcac ccaggcccag
      181 ctggaggaga tcgcgcagac caagatgccc gacctcaacg cctacgacct cgaagccgcc
      241 aagctccagg tcgccggcac cgcccgctcc atgggcgtgg aagtgaagaa gtagcagccc
      301 ctctggctgc gacccactcc tccggaagga cctcgtccgg agtgacggca atcgacgagg
      361 gagtctgaaa ggaccagtca ccatggctgc aggaaagcgc taccgcgacg ccaccgcccg
      421 ctacgaccgg gatgccaacc acgccccggc cgaggccctc gatctggccc ggtccctcgc
      481 gaacgccaag ttcgacgaga ccatcgacct gatcgcccgc ctcggcgtcg acccccgcaa
      541 ggccgaccag atcgtccgcg gcaccgtcgc cctcccgtcg ggcaccggca ccgacgtccg
      601 cgtcgccgtg ttcgcccagg gcgacgccgc ccaggcggcc cgtgacgccg gcgccgagca
      661 cgtcggcggc gacgacctcg ccgagcaggt cgacggcggc atgctcgact tcgacgtggc
      721 catcgccacg cccgacatga tgcccaccgt cggcaagctg ggccgcgtgc tcggcccccg
      781 cggcctcatg cccaacccca agaccggcac cgtgaccacc gacgtcgcca aggccgtcga
      841 ggagttcaag ggcggcaagg tcgagtaccg caccgaccgc tacggaaacg tgcacgtgcc
      901 gatcggcaag tccagcttcg acagcgcgca gctggccgcc aacttctggg ccgtggtcga
      961 agagctccag cgggtccgcc ccgcctcggc caagggtcgc tacttccgcc gcatcggcgt
     1021 gagctcgacc atgggccccg gcatccgcat cgacaccggc cgcctcaagc gcgagaccac
     1081 cgactagcga gttggtgctg cggcggctgc cgccgtagca tcgcccactt catcacaatc
     1141 gaacctgctc gccgcagaca tccggtgcga ccccacgggt cgcgcaatgg gccaggggcc
     1201 cgcccgacga ggcgacacct cctgaaccgg ctctgccggc tctgcccgtg tgcgagcccg
     1261 agacagaacg aaggaggtgt ccatggagaa ccccagaccg gagaaggttg cggtggtcga
     1321 cgaggtgcga gagcgcttcg acggcaccag cgctgccatc ctcaccgagt accgcggcct
     1381 cagcgtcggc cagctggccg acctgcgcac ccgactgcgg ggcgccggcg gcgacttcaa
     1441 ggtgtacaag aacaccttgg tgcgcttcgc cgcccgcgac ctcgacctcg agatcgaaga
     1501 gctgctcacc ggcccgaccg ccatcgcgtt cgtgaccgag aggcccgacg gtgaacccgg
     1561 cgacgccgtc gatgtcgcca aggtcctgaa ggacttcggc aaggagttcc cggcactggt
     1621 cgtcaagggc ggcctgctgg gcgacaagct cctcaccgcc gacgacacca aggccctggc
     1681 cgacgtcgag ccccgcgagg tcctgctggc caagctggcc ggcgccatgg ctgccccgat
     1741 gcagcagctc gccggactgc tccaggccct gccccgcaac atggcctacg gcctcaaggc
     1801 cctcatcgac cagggcggtg ccgaaggtgc ccccgccgac gctgctcccg cggccgacgc
     1861 cgacgagagc acgaccgacg agagccccac cgacgaggcc ccggcacccg ccgaggccga
     1921 gaacaccacc gacgacgccc cggcaaccgc cgaggacgac acccccgagg ccgaggacac
     1981 ccccgcggcc gaggcggccc ccgctgccga caccgacacc caggagagct gaaccatggc
     2041 caccaaggaa gagatcctcg acgccatcgc caacatgacc gtcctcgagc tgagcgagct
     2101 cctgtcggac ttcgaggaga agttcggcgt gaccgccgcc gcccccgtgg ccgtggccgc
     2161 agccggtggc cccgccgccg gtgctggcga ggccgaagag gagcaggacg agttcgacgt
     2221 catcctcaag ggtgccggcg acaagaagat cggcgtcatc aaggaggtgc gcgccctcac
     2281 caacctcggt ctcaaggagg ccaaggagct ggtcgacggc gccccgagcc ccgtgctc
//
LOCUS       DATISU010000299         5749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_858863, whole genome shotgun sequence.
ACCESSION   DATISU010000299 DATISU010000000
VERSION     DATISU010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858863"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..730)
                     /locus_tag="VLR27_11990"
     CDS             complement(122..730)
                     /locus_tag="VLR27_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HSP04219.1"
                     /translation="MTPPVRTGRIGGQMVAIVEGLANRMRIPALVQRHDSVAVMGAFA
                     FVNGCVAIGVMSFGAFVTGSPFVFPSLGPTAFLHFYTPMAPAASPRNTLCGHAVGVAA
                     AVISLLAFGLTGEGPALEDGMTLARVGAVALSLGLTSGAMVWLRVPHPPAGATTMIIS
                     LGILTEAAHLAVLLFAVVLLTIQAFLINRAAGVPYPRWSPAS"
     gene            complement(727..1062)
                     /locus_tag="VLR27_11995"
     CDS             complement(727..1062)
                     /locus_tag="VLR27_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04220.1"
                     /translation="MADGPRPIRPLPSTPAPESERGLATSLVTRIVIVATIVLGQLWA
                     LTVALEASLLDEDGQAWLLAGFSIVSFVVTLFLTTVEAPRRSSALRGRAASGSGRYVS
                     RPAGDGRRP"
     gene            complement(1062..1583)
                     /locus_tag="VLR27_12000"
     CDS             complement(1062..1583)
                     /locus_tag="VLR27_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HSP04221.1"
                     /translation="MTDRTNPSDPALVDERIQRLTTDALVTRRGYLKILGVLSGGLAA
                     GNVAVALGAFERRTEGAEVEMVIADDVDAIPVGGEVRFSYPTDNDPAVLLRLEDELFV
                     AYSAVCTHLACEVLWRQDERDLFCPCHDGHFDPSDGAPTAGPPKRPLPEIVLERRGNS
                     IVALREGRIREEH"
     gene            complement(1580..2152)
                     /locus_tag="VLR27_12005"
     CDS             complement(1580..2152)
                     /locus_tag="VLR27_12005"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSP04222.1"
                     /translation="MSLERSIFVDPSRCIACGACVAACRECDSHRGKSMMNLDFLNRG
                     ESTLALPTVCMHCTDPVAPCAQVCPVDAILVSDDGVVHSPDPSRCIGCQNCVHGCPWG
                     VPKFDVEEQLAHKCNLCYDRTSVGLGPMCATVCPSDALYYGTADEVLASRPNRRVVDV
                     FSFGAQELQSGNWVVVPDEFSVLTFPMVGP"
     gene            complement(2149..4380)
                     /locus_tag="VLR27_12010"
     CDS             complement(2149..4380)
                     /locus_tag="VLR27_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HSP04223.1"
                     /translation="MSPQRRRTAFRLSTPPGVDDFHEAGARPAGGWETDESDERWVST
                     HCAFCGVQCGMHLRVAGHEVIGVEPRTDTHNKGKLCPKGVAAYQQISHDDRLTHPLVR
                     DGGVLRRATWDEALDRVVDGIRGIQAAGGPDAMATYGGASMVNEKTYLLGKFARVALG
                     TRFTDYNGRMCMVSAGAANYRSFGIDRSANPLSDVAEADVLLVLGANVPETFPVYIRW
                     FWEAMDRGATMICVDPRETTLARVAQLHLPIRPGTDGALLNAMLHVVVRDGMVDEDFV
                     AAHTTGFAEASAAVADWTPERAAEVCGVPAADIERAAQLYGRADKAMVSHARGMEHQV
                     MGARNAMAAINLCLATGNIGRKGAGYGTITGQGNGQGGREHGQKCDQLPGQRHFTDPG
                     AIEHVAGVWGIDPADMPGPGEPIFAQLDRMEAGEIRGLLNICSNPMVSWPDEARTRRI
                     LEGLELYVVIDIFLSESALLADVVLPGSAWAESEGVVANSDALVCKINKAVDPPGEAK
                     PDMWILAEIARRLGKGEHFPDQTPREVFDELRRASAGGRADYYGITYERLEAEGPIAW
                     PCPTEDHPGTPRLFEDLRFNTDDGKAHFNAVEVVPPAEEPDGEYPIRLTTGRTVAHYL
                     SGNQTRRIGYLTDQTPSPWVEIHPNTAAEHGIVDGAPVRVTSRRGTTVLPAMVVRTIR
                     QDTVFIPYHWASPVAANQLTKSSFDPISWIPAFKTSAVRIEASEEPAMAVTPPPVFEG
                     GGR"
     gene            complement(4377..5462)
                     /locus_tag="VLR27_12015"
     CDS             complement(4377..5462)
                     /locus_tag="VLR27_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04224.1"
                     /translation="MAGPHRSSTTADHRPHARERLIGAVAALVLVALIWLGSNQLANF
                     DSALIGYAVGIVALTYAVVMRYARWLRMPSTGRYWRRGWQLTTSWSSFRALPGLLPRA
                     IAGQLLLQGFIRRRGWVRWIGHQCLFWGVIGATLITFPLVFGWLAFKLEPGTQEMYRM
                     WVFGFPTITFDSTSFLGWSLFHALNYTAVLVIIGCAIFLWRRFRDRAAIAGQRFGYDM
                     VPLLMLIVISVTGLLLTVSASLFHGSYYDFLVVIHMAAVVLSLVFIPFGKFFHVLQRP
                     ASVGIQMYAEINGREGRRPCAKCGEPLAPDLFVQDLQSTLQDLGQDYRLDDASMPAGH
                     LTELCPRCKRVARAEGYFAHDGSSFAR"
     gene            complement(5512..>5749)
                     /locus_tag="VLR27_12020"
     CDS             complement(5512..>5749)
                     /locus_tag="VLR27_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04225.1"
                     /translation="LLGDPSYDGRILKAHERVDAQQPFDAELFDRWYRLFVESVDSGW
                     SGSQAERAKRHAAHMAGVISRRLTGEDFEVRRIS"
BASE COUNT          864 a         1971 c         2135 g          779 t
ORIGIN      
        1 cctcacgacc acgtcgttgc cccggaccgg ctccggcctc acggccctgg cgatcggtgg
       61 cgccgtctcg ctcctgctgg gcgccgcgat ggtgcgccgg gccgcgacgg cgtagcccgg
      121 atcaggacgc cggcgaccac ctcgggtagg ggacgccggc ggcccggttg atcaggaacg
      181 cctggatggt gagcagcacc acggcgaaca gcagcacggc gaggtgcgcg gcctcggtga
      241 ggatgccgag cgagatgatc atcgtggtcg cgccggcggg cgggtgcggc acccgcagcc
      301 agaccatcgc cccgctggtg aggcccaggc tcagcgccac ggcaccgacc cgggcgaggg
      361 tcatgccgtc ctcaagcgcc gggccctccc ccgtcaggcc gaacgccagg aggctgatga
      421 ccgcagcggc gaccccgacc gcgtgcccgc acaaggtgtt gcggggcgac gctgccggcg
      481 ccatcggcgt gtagaagtgg aggaaggccg tggggccgag gctggggaac acgaacggcg
      541 agccggtgac gaaggcaccg aagctcatca ctccgatggc gacgcagccg ttgacgaacg
      601 cgaacgcccc catcaccgcg acggagtcgt ggcgctggac gagcgccggg atgcgcatcc
      661 ggttggccag accctcgacg atggcgacca tctggccgcc gatgcggccg gtccggaccg
      721 gtggcgtcac ggcctgcgac cgtcccccgc cgggcgggag acgtagcgcc cgctgccgct
      781 ggccgcccgc ccccgcaggg cgctgctccg ccgaggcgcc tccacggtgg tgaggaacag
      841 ggtgaccacg aacgacacga tcgagaaccc ggcgagcagc caggcctgcc cgtcctcgtc
      901 gagcagcgac gcttcgagcg cgacggtgag cgcccacagc tgcccgagca cgatcgtggc
      961 gacgatgacg atgcgggtca ccaggctcgt ggcgaggccg cgctcgctct cgggggcagg
     1021 cgtgctcggc agtggccgga tgggccgggg accgtcagcc atcagtgctc ctcgcggatg
     1081 cgtccctcgc gcagggccac gatcgagttg ccccgccgct ccagcacgat ctcggggagc
     1141 ggacgcttcg gcggcccggc cgtgggggca ccgtcgctcg ggtcgaagtg cccgtcgtgg
     1201 cacgggcaga agaggtcgcg ctcgtcctgc ctccacagca cctcgcaggc caggtgggtg
     1261 cacaccgccg agtaggcgac gaagagctcg tcctcgagcc ggagcagcac cgccgggtcg
     1321 ttgtcggtcg ggtacgagaa gcggacctcg ccgcccacgg ggatggcatc gacgtcgtcg
     1381 gcgatgacca tctcgacctc ggcgccctcg gtccggcgct cgaacgcacc gagggccacg
     1441 gcgacgttgc cggcggccag accgccggag agcaccccga ggatcttgag gtacccgcgc
     1501 cgggtgacca gcgcatcggt ggtcagccgt tggatgcgct cgtcgacgag cgcggggtcg
     1561 gacgggttgg tgcgatcggt catgggccca ccatcgggaa cgtgaggacg gagaactcgt
     1621 cggggacgac cacccagttg ccgctctgca gctcctgggc gccgaagctg aacacgtcga
     1681 ccaccctgcg gttcgggcgt gacgccagca cctcgtccgc ggtgccgtag tacagggcat
     1741 cggaggggca gaccgtggcg cacatgggac cgaggccgac ggacgtgcgg tcgtagcaga
     1801 ggttgcactt gtgcgcgagc tgctcctcga cgtcgaactt cggcacgccc cacgggcagc
     1861 cgtgcacgca gttctggcag ccgatgcagc gcgacggatc cggcgagtgc acgacgccgt
     1921 cgtcgctgac gaggatggcg tcgaccgggc acacctgcgc gcacggggcc accgggtcgg
     1981 tgcagtgcat gcacacggtg ggcagggcca gggtcgactc accccggttc aggaagtcga
     2041 ggttcatcat cgacttgccc cggtggctgt cgcactcccg gcaggcggcc acgcacgcgc
     2101 cgcacgcgat gcagcgggac gggtcgacga agatcgagcg ctcgaggctc atcggccacc
     2161 tccctcgaac acgggtggtg gcgtgacggc catcgccggc tcctcgctcg cctcgatgcg
     2221 caccgccgag gtcttgaacg ccgggatcca gctgatcggg tcgaacgacg acttcgtcag
     2281 ctggttcgcc gcgaccggcg acgcccagtg gtacgggatg aagacggtgt cctggcggat
     2341 cgtgcgcacg accatggccg gcagcacggt cgtgccccgc cggctcgtga cccgcaccgg
     2401 ggcgccgtcg acgatgccgt gctcggccgc ggtgttgggg tggatctcca cccagggcga
     2461 cggcgtctgg tcggtgaggt agccgatgcg ccgggtctgg ttgcccgaca ggtagtgcgc
     2521 cacggtgcga ccggtggtga gccggatcgg gtactcgccg tcgggctcct ccgccggcgg
     2581 gacgacctcg accgcgttga agtgcgcctt gccgtcgtcg gtgttgaagc ggaggtcctc
     2641 gaacagccgc ggcgtgccgg ggtggtcctc ggtcgggcac ggccaggcga tgggaccctc
     2701 ggcctcgagc cgctcgtagg tgatgccgta gtagtcggcc cgcccgccgg ccgacgcccg
     2761 gcggagctcg tcgaacacct cgcggggcgt ctggtcgggg aagtgctcgc ccttgccgag
     2821 ccggcgggcg atctcggcca ggatccacat gtcgggcttg gcctcacccg gcgggtcgac
     2881 cgccttgttg atcttgcaga cgagggcgtc gctgttggcg accacccctt cggactccgc
     2941 ccacgccgaa ccgggcagca ccacgtcggc gagcagggcg gactcggaca ggaagatgtc
     3001 gatcaccacg tagagctcga ggccctcgag gatgcgacgg gtccgggcct cgtcgggcca
     3061 ggacaccatc gggttggagc agatgttgag caggccccgg atctcgccgg cctccatgcg
     3121 atcgagctgg gcgaagatcg gctcgcccgg ccccggcatg tcggcgggat cgatgcccca
     3181 caccccggca acgtgctcga tggcgccggg gtcggtgaag tggcgctgcc cgggcagctg
     3241 gtcgcacttc tggccgtgct cgcggccgcc ctgcccgttg ccctggccgg tgatggtgcc
     3301 gtagcccgcg cccttgcggc cgatgttgcc ggtggccagg cacaggttga tcgccgccat
     3361 ggcgttgcgg gcgcccatca cctggtgctc catgccccgg gcgtggctca ccatggcctt
     3421 gtcggcccgg ccgtacagct gggcggcccg ctcgatgtcg gcggccggca ccccgcacac
     3481 ctcggcggcc cgctcgggcg tccagtcggc cacggcggcc gacgcctcgg cgaacccggt
     3541 ggtgtgggcg gccacgaagt cctcgtcgac catcccgtcg cgcacgacga catggagcat
     3601 cgcgttgagc agcgcaccgt cggtgccggg acggatcggc aggtgcagct gggcgactcg
     3661 ggcgagcgtg gtctcccggg ggtcgacgca gatcatggtg gcgccgcggt ccatggcctc
     3721 ccagaaccag cggatgtaca cggggaaggt ctcggggacg ttggcgccga gcacgagcag
     3781 cacgtccgcc tcggcgacgt cgctcagggg gttggcgctg cggtcgatcc cgaaggaccg
     3841 gtagttggcc gcccccgccg agaccatgca catccggccg ttgtaatccg tgaaccgggt
     3901 gcccagcgcc acgcgcgcga acttgccgag caggtacgtc ttctcgttga ccatcgacgc
     3961 cccgccgtag gtggccatcg catcgggacc gccggctgcc tggatcccgc ggatgccgtc
     4021 gacgacccgg tcgagcgcct cgtcccaggt cgcccgtcgc aggacgccgc cgtcgcgcac
     4081 cagcgggtgg gtgagccggt cgtcgtggct gatctgctgg taggcggcca cgcccttggg
     4141 gcagagcttg cccttgttgt gggtgtcggt gcggggctcg accccgatga cctcgtggcc
     4201 ggcgacccgc aggtgcatgc cgcactgcac gccgcagaac gcgcagtgcg tggagaccca
     4261 gcgctcgtcg gactcgtcgg tctcccaccc gccggccggc ctcgcgcccg cctcgtggaa
     4321 gtcgtcgacg ccgggcggcg tggagaggcg gaaggcggtg cgtcggcgct gggggctcat
     4381 cgggcgaagc tcgatccgtc gtgggcgaag tacccctcgg cccgggcgac ccgcttgcag
     4441 cgggggcaca gctcggtgag gtggccggcc ggcatggacg cgtcgtccag ccggtagtcc
     4501 tggccgaggt cctgcagggt cgactgcagg tcctgcacga agaggtcggg cgcgagcggc
     4561 tcaccgcact tggcgcaggg gcgacggccc tcccgaccgt tgatctcggc gtacatctgg
     4621 atgccgaccg atgccggtcg ctgcagcacg tggaagaact tcccgaacgg gatgaacacc
     4681 agcgacagca ccaccgccgc catgtggatg acgacgagga agtcgtagta cgagccgtgg
     4741 aagaggctgg ccgagaccgt gaggagcagc ccggtgaccg agatgacgat gagcatcagc
     4801 agcgggacca tgtcgtagcc gaagcgctgc ccggcgatgg cggcccggtc gcggaagcgg
     4861 cgccacagga agatggcgca gccgatgatg acgaggacgg cggtgtagtt gagggcgtgg
     4921 aacagcgacc acccgaggaa cgacgtggag tcgaacgtga tggtcgggaa cccgaacacc
     4981 cacatccggt acatctcctg ggtccccggc tcgagcttga acgccaacca cccgaacacg
     5041 agggggaagg tgatgagggt ggcgccgatc acgccccaga acaggcactg gtgcccgatc
     5101 cagcggaccc acccgcgccg gcggatgaag ccctgcagga ggagctggcc ggcgatggcc
     5161 cgcggcagca gcccgggcag ggcacggaac gacgaccacg acgtggtgag ctgccacccc
     5221 cgccgccagt agcgaccggt ggacggcatc cgcagccacc gggcgtaccg catcaccacg
     5281 gcgtaggtga gggcgacgat gccgaccgcg tagccgatga gtgcggagtc gaagttggcg
     5341 agctggttcg acccgagcca gatgagcgcc accaggacga gcgcggccac cgcaccgatc
     5401 aggcgctcgc gagcgtgggg ccggtggtcg gcggtggtgc tcgatcggtg cgggcctgcc
     5461 atgtcgatcc ccgaggtcgc gtgcggcgat cgccaccata gacacatcgc actacgagat
     5521 tctgcggacc tcgaagtcct cgccggtcag gcgacgggag atcaccccgg ccatgtgggc
     5581 ggcgtggcgc ttggcgcgct cggcctgaga ccccgaccag ccgctgtcga ccgactcgac
     5641 gaagagccgg taccaccggt cgaacagctc agcgtcgaag ggctgctggg cgtcgacccg
     5701 ctcgtgggcc ttcaggatgc ggccgtcgta gctcgggtca cccaggagc
//
LOCUS       DATISU010000300         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_206485, whole genome shotgun sequence.
ACCESSION   DATISU010000300 DATISU010000000
VERSION     DATISU010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206485"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VLR27_12025"
     CDS             <1..663
                     /locus_tag="VLR27_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HSP04226.1"
                     /translation="VEELLGGEGGFQPEVQARTREAADALEAAGAKVDVVSVPASTYG
                     LSAYYLIAPAEASSNLARFDGVRFGLRDERATMGETYDATRTAGFGAEVKRRIMLGTY
                     ALSSGYYDAYYGKAQRVRTMIIRDFDAAYERFDVLLSPASPCVAFPLGDKTADPLTMY
                     LNDVCTIPSSLAGHPAMSVPFGTGADDLPVGIQVMAPALGEEPMFRVAAALEAAAPGG
                     AR"
     gene            660..2105
                     /gene="gatB"
                     /locus_tag="VLR27_12030"
     CDS             660..2105
                     /gene="gatB"
                     /locus_tag="VLR27_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HSP04227.1"
                     /translation="MTATETSFTELPHGYRLVVGLEVHVELHTATKLFCGCPNQFGDD
                     PNTNVCPVCLGLPGSMPVLNAQAMELAMRLGRALHCEVRPSVFARKNYFYPDMPKDYQ
                     VSQYDQPTNVDGHLDLPDGTRIGIERAHIEEDTGKSTHVGGGGRIHDAAYSLVDYNRC
                     GVPLVEIVSRPDFRTAEQAKAYVNELRAILVATGVSDAKMEEGSLRVDANVSVHQPDT
                     PFGTRCEIKNINSVRSLGRAIEYEALRQVDLLEAGERVIQQTRHWNEDEGRTHMLRSK
                     EEAEDYRYFPEPDLVPMVPEQARIDAIDAALPRLPAERRSALAEAAGRTLPDESVTIV
                     VDRGLDELALAAVAAGADADRTLTHLQHDLDPDHAGNVPAAHLAQLVELETSGKLTAS
                     QAKQVVAEMGETGKAPEAIAAERGFEAMDTSELEGIVDDAIAANGDAWEKYCAGEQKA
                     QGAIVGHVMKATRGQADGKLVSQILEQRRSA"
     gene            2102..2593
                     /locus_tag="VLR27_12035"
     CDS             2102..2593
                     /locus_tag="VLR27_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04228.1"
                     /translation="MKLASALGVAVCALVLATACGDGDAEDATEPTPDPSEEPTASTA
                     PPSFEGDPDSEFCQRSRAAAEEPVLDPFEAGLEPREVELRFRALAQRFEGFAEIAPEP
                     LAEDLALLDATFDDLAVVLADADYDFARLAADEVDVSVFDDPALAVVADRLTAYQDQV
                     CER"
BASE COUNT          389 a         1030 c          845 g          331 t
ORIGIN      
        1 gtcgaggagc tgctcggcgg cgagggcggg ttccagcccg aggtccaggc ccgcacccgc
       61 gaggccgccg acgcgctcga ggccgccggc gcgaaggtcg acgtggtctc ggtgccggca
      121 tccacctacg gcctctccgc ctactacctg atcgccccgg ccgaggcgtc ctccaacctg
      181 gctcgcttcg acggcgtgcg cttcggcctg cgcgacgagc gggcgacgat gggggagacc
      241 tacgacgcga cccgcaccgc ggggttcggt gccgaggtca agcggcgcat catgctcggc
      301 acctacgccc tctcctcggg ctactacgac gcctactacg gcaaggccca gcgggtgcgc
      361 acgatgatca tccgcgactt cgacgccgcc tacgagcgct tcgacgtgct gctcagcccg
      421 gcctcgccgt gcgtggcctt ccccctcggc gacaagaccg ccgatccgct caccatgtac
      481 ctcaacgacg tgtgcacgat cccgtccagc ctggccggcc acccggccat gtcggtcccg
      541 ttcggcaccg gcgccgacga cctgcccgtg ggcatccagg tcatggcccc cgccctgggg
      601 gaggagccga tgttccgggt cgccgccgcg ctcgaggccg ccgcgccggg aggtgcccga
      661 tgaccgccac cgagacgtcc ttcaccgagc tgccccacgg ctaccgcctc gtggtgggcc
      721 tcgaggtcca cgtcgagctg cacaccgcca ccaagctgtt ctgcggctgc cccaaccagt
      781 tcggggacga ccccaacacc aacgtctgcc cggtgtgcct cggcctgccc ggttccatgc
      841 cggtgctcaa cgcccaggcg atggagctgg ccatgcgcct cggccgcgct ctgcactgcg
      901 aggtgcggcc ctcggtcttc gcccggaaga actacttcta cccggacatg ccgaaggact
      961 accaggtctc ccagtacgac cagccgacca acgtcgacgg ccacctcgac ctgcccgacg
     1021 gcacccgcat cgggatcgag cgggcccaca tcgaggagga caccggcaag tccacccacg
     1081 tcggcggtgg cggacgcatc cacgacgccg cctactccct cgtggactac aaccgctgcg
     1141 gcgtcccgct ggtcgagatc gtctcccggc ccgacttccg caccgccgag caggccaagg
     1201 cctacgtcaa cgagctgcgc gccatcctcg tggccaccgg cgtgtccgac gccaagatgg
     1261 aggagggctc gctgcgcgtc gacgccaacg tgtccgtcca ccagcccgac acgcccttcg
     1321 gcacccggtg cgaaatcaag aacatcaact cggtgcgctc gctggggcgg gccatcgagt
     1381 acgaggcgct gcgccaggtc gacctgctcg aggccggcga gcgggtcatc cagcagaccc
     1441 gccactggaa cgaggacgag ggtcgcaccc acatgctgcg ctcgaaggag gaggccgagg
     1501 actaccggta cttccccgag cccgatctcg tgccgatggt ccccgaacag gcccgcatcg
     1561 atgccatcga cgccgccctg ccccgcctgc ccgccgagcg ccgcagcgcc ctcgccgagg
     1621 ccgccgggcg caccctgccc gacgagtcgg tgaccatcgt ggtcgaccgc ggcctcgacg
     1681 agctggcgct cgccgccgtg gccgcgggcg ccgacgccga tcgcaccctc acccacctcc
     1741 agcacgacct cgaccccgac cacgccggca acgtccccgc cgcccacctg gcccagctgg
     1801 tcgagctcga gaccagcggc aagctcaccg ccagccaggc caagcaggtg gtggccgaga
     1861 tgggcgagac cggcaaggcc cccgaggcca tcgccgccga gcgcggcttc gaggccatgg
     1921 acaccagcga gctggagggc atcgtcgacg acgccatcgc cgccaacggt gacgcgtggg
     1981 agaagtactg cgccggcgag cagaaggccc agggcgccat cgtcggtcac gtcatgaagg
     2041 cgacccgggg ccaggccgac ggcaagctcg tgtcccagat cctcgagcag cgtcggtcgg
     2101 cgtgaagctc gcctcggcgc tcggcgtcgc ggtctgcgcg ctggtgctgg ccacggcctg
     2161 cggcgacggc gacgccgagg acgccaccga gccgacgccg gacccctccg aggagcccac
     2221 cgccagcacg gcgccgccca gcttcgaggg cgaccccgac tccgagttct gccagcgcag
     2281 ccgggctgcg gccgaggaac cggtgctcga cccgttcgaa gccgggctcg agccccgtga
     2341 ggtggagctg cgcttccggg ccctggccca gcgcttcgag ggcttcgccg agatcgcccc
     2401 cgagcccctc gccgaggacc tggcgctcct cgatgccacc ttcgacgacc tcgccgtcgt
     2461 gctggccgac gccgactacg acttcgcccg cctcgcggcc gacgaggtcg acgtgtcggt
     2521 gttcgacgac cccgccctcg ccgtcgtcgc cgaccgcctc accgcctacc aggaccaggt
     2581 ctgcgagcgc tgacc
//
LOCUS       DATISU010000301         6054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_811128, whole genome shotgun sequence.
ACCESSION   DATISU010000301 DATISU010000000
VERSION     DATISU010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811128"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1011)
                     /locus_tag="VLR27_12040"
     CDS             complement(37..1011)
                     /locus_tag="VLR27_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP04229.1"
                     /translation="MRRSRVLLAVAAVGGAVAAKRALDGHTARWDTNPDHCDGDPLGL
                     PATTEIEVTAADGARLRGHRCGDAGGPTVLLVHGYIESVEFWAPVVRRLAEAGLDVVL
                     VDQRGHGASERGTAPFDTDTLAGDLRSWVEELDVSDAILVGHSMGGVSAMAFSATHPD
                     VAAGRLRSQVLVATLAFPEPLFGLPDIDVAPHRVVGLLERVMQRETLALLFVSRVFGT
                     WPTRPALEATRQGIMRTDRQTRIDAMAMLRSFDLRPRLASIELPTVVVAGTHDQLTPL
                     AGNELIAESISGARLEVLPGMGHMLMFEAPDAVADAIVQATKPADAVR"
     gene            1080..1289
                     /locus_tag="VLR27_12045"
     CDS             1080..1289
                     /locus_tag="VLR27_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04230.1"
                     /translation="MGATDDLPDVLRFDCPRCRREVETAYYGPCETCLGELRVRYQAE
                     RTEVAAVAYEPKMNVTPNAVALKDD"
     gene            1290..2717
                     /locus_tag="VLR27_12050"
     CDS             1290..2717
                     /locus_tag="VLR27_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HSP04231.1"
                     /translation="MPQPRRRVRPIEPVVGDGLAAIRHELGVPAAFPDEVLAAAATLG
                     PAPEQGRVDRRDLPMCTIDPEGSTDLDQALAVEAVDGGHVVWYAIADVAAFVVPGDPI
                     DVESRRRGVTLYSPDGRAPLHPPALSEDLASLLPDQDRPAVLWRLRLDPDGQLVEVTV
                     GRATVRSRVQLSYEEAQQRIDAGDDELAPLAVVGEQRLAIERDRGGVSLPVPDQEAVR
                     DGDGYRLAYRAQLPVERWNAQLSLLCGMAAADLMLAGGWGLLRTLPPADEESLAALRR
                     HAAALGVPWPERARYADVIHDVDPSDPAAAAFLIQATRLFRGADHVPLRPGSAAPDDV
                     LVHAAIAAPYAHVTAPLRRLGDRFATECVLATVHGKEPPAWARDALEELPELLRSSMA
                     RANELDRAVVDHLEALVLSSHVGERFRAVVVDQRKGAAVVQLREPAVVATVPGEHPLG
                     EEVDVEVVSADPVARTVELAVRGSR"
     gene            complement(2714..4150)
                     /locus_tag="VLR27_12055"
     CDS             complement(2714..4150)
                     /locus_tag="VLR27_12055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HSP04232.1"
                     /translation="MADDRRPDLDRVPDIADAPVGWDPGKRRFTGGSDASEPTPPRTG
                     SRGVTPPGATADTRRFVIPAPPAGGRSTPPPRRTPEVVLPSSAPADPVGGRTMVAPAP
                     STPAPSADRPARVVVPAPGAAPKAAAPAAAKAASAKSTPAARDRQPKRPADRRWPPSR
                     RTVVRTLVGVVLVLALFAVGGLLYGYQQFQSIERVELGDVLASADGTNYLIVGSDTRS
                     GISADDPNAGAILGPDAQGGEPERSDTIMILRVDGEGSRMLSVPRDLLVTISETGQTT
                     RINSAFNGGPRRLIATLRDQLQLPIHHYLEIDFVAFRDLVDALGGITIDFPHPASDHM
                     SGLNVPEAGPQVLDGTQALAYVRSRYYTETIDGRQVTEPTGDIGRTVRQQQFLSAVVG
                     EMSGERNPLRLARMTSSMADGMRIDDQMGYLDAVRLALRLRGMGLEPTSLPTTNRTTS
                     SGMAVLDLVQPGADEVLARFGSPGATIG"
     gene            4375..4623
                     /locus_tag="VLR27_12060"
     CDS             4375..4623
                     /locus_tag="VLR27_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04233.1"
                     /translation="MPITERLRRARLRRDARARGLEVALGLGDEPPAERRPASLVDLR
                     CARCGGAGRLESLDLRTSRGLGRCPSCGTTWSIIYPPG"
     gene            complement(4625..>6054)
                     /locus_tag="VLR27_12065"
     CDS             complement(4625..>6054)
                     /locus_tag="VLR27_12065"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HSP04234.1"
                     /translation="SLWRALVQDPEAGPMVAGRFDHVLVDEYQDTNALQADVLAAMAD
                     AGATVTAVGDDAPAIYGFRGGTVRNILDFPSRFDAEVVALTRSHRSTPEVVTVANRIW
                     AAATERHDKELVATRAPGPRPALVTAGDEHAEARGVCERLLESVERGIPLRRQAVLFR
                     TGHHADLLEVELTVRRIPFVKFGGLKFLEAAHVKDLLAMCRVAVNPRDDLAWFRILQR
                     IDGVGPGIARRVVTGLDAADGSLARVVDSGVVPPRSVDEVAGLASAISTAADGDLATG
                     ERIDACRAWLDPHLDRFGDGAARRADLARLAELAAGTPDLETFLTELTLDPPASTGDL
                     AGPPHLDDDVLTLSTIHSAKGGEWDIVHLIHAADGNLPSDLSTGDPEQLEEERRLLYV
                     AVTRARDELHVHVPLRFHHHRNRLDDAHSTGQRSRFLTPDVVEAMDEVVAVRAASTSE
                     LDLPVPGSRRSAVAAADAELAALLG"
BASE COUNT          759 a         2047 c         2477 g          771 t
ORIGIN      
        1 cgaaggtgtc ggacacgtga tcctgcatcg ggccgctcac cggacggcgt cggccggctt
       61 ggtggcctgc acgatggcgt cggcgacggc gtcgggggcc tcgaacatga gcatgtggcc
      121 catgccgggg agcacctcga ggcgggcccc ggagatcgac tcggcgatga gctcgttgcc
      181 ggcgagcggg gtgagctggt cgtgggtgcc ggccacgacc acggtgggca gctcgatcga
      241 tgccaggcgc ggtcgcaggt cgaagctgcg gagcatcgcc atggcgtcga tgcgggtctg
      301 gcggtcggtg cgcatgatgc cctggcgggt ggcctccagc gctgggcggg tcggccaggt
      361 gccgaacacc cgggacacga acagcagcgc cagcgtctcg cgctgcatca cccgctcgag
      421 cagacccacc acccggtggg gggcgacgtc gatgtcgggc agcccgaaca gcggctcagg
      481 gaaggcgagc gtggcgacca gcacctggct gcggaggcgg ccggcggcga cgtcgggatg
      541 ggtggcgctg aaggccatgg cgctgacgcc gcccatggag tggcccacga ggatggcgtc
      601 gctgacgtcg agctcctcga cccacgagcg gaggtcgccg gcgagggtgt cggtgtcgaa
      661 gggcgcggtg ccgcgctcgg aggcgccgtg tccccgctgg tcgacgagca cgacgtcgag
      721 gccggcctcg gccaggcggc gcacgaccgg ggcccagaac tccaccgact cgatgtagcc
      781 gtggacgagc aggacggtgg ggccgccggc atcgccgcag cggtggcccc ggaggcgggc
      841 gccgtcggcc gcagtgacct cgatctcggt ggtggcgggc agcccgaggg ggtcgccgtc
      901 gcagtggtcg gggttggtgt cccagcgcgc cgtgtggccg tcgagcgcgc gcttggcggc
      961 gacggcgccc ccgacggcgg cgacggcgag caggacgcgg gaacggcgca taccggccag
     1021 tgtggccacg tcgggcgcgt ccgcgccacc gtcgccgacg accggtaccg tcgggcgcca
     1081 tgggcgcgac cgatgacctc cccgacgtgc tgcgcttcga ctgcccccgc tgccggcggg
     1141 aggtcgagac cgcctactac ggcccgtgcg agacgtgcct gggcgagctg cgtgtgcggt
     1201 accaggccga gcgcaccgag gtcgccgcgg tcgcctacga gcccaagatg aacgtcacgc
     1261 ccaacgcggt ggcgctcaag gacgactgag tgccccagcc gcgtcgtcgc gtccgcccca
     1321 tcgagccggt ggtgggcgac ggcctcgccg ccatccggca cgagctcggg gtgccggcgg
     1381 cgttccccga cgaggtcctg gccgcggcag cgacgctcgg tccggcacca gagcagggcc
     1441 gggtggaccg gcgcgacctg ccgatgtgca ccatcgatcc cgagggcagc accgacctcg
     1501 accaggcgct ggccgtcgag gccgtcgatg gtggccacgt cgtctggtac gccatcgccg
     1561 acgtcgccgc cttcgtggtc cctggcgacc cgatcgacgt cgagtcccgt cggcgaggtg
     1621 tgacgctgta ctcgcccgac ggacgggcgc cgctgcaccc gccggcgctg tcggaggacc
     1681 tggccagcct gctgccggac caggaccggc cggccgtgct gtggcgcctg cggctcgacc
     1741 ccgacgggca gctggtggag gtgacggtcg gccgggcgac ggtgcggtcc cgggtgcagc
     1801 tgtcctacga ggaggcccag cagcgcatcg atgcgggcga cgacgagctg gcgccgctcg
     1861 cggtggtggg ggagcagcgg ctggcgatcg agcgcgaccg gggtggggtg agcctgccgg
     1921 tgccggacca ggaggccgtg cgcgacgggg acggctaccg cctcgcctac cgggcccagc
     1981 tcccggtgga gcggtggaac gcccagctct ccctgctgtg cggcatggcc gccgcggatc
     2041 tcatgctcgc cggtggctgg gggctgctgc ggacgctgcc gccggcggac gaggagtccc
     2101 tcgccgccct gcgccggcac gccgccgcgc tcggcgtgcc gtggccggag cgcgcccggt
     2161 acgccgacgt catccacgac gtcgacccga gcgacccggc ggccgccgcg ttcctcatcc
     2221 aggccacccg tctgttccgg ggtgccgacc acgtgccgct gcgcccgggt tcggccgccc
     2281 ccgacgacgt gctggtgcac gccgccatcg ccgcgccgta cgcccacgtg accgcaccgc
     2341 tgcgccggct cggggatcgc ttcgccaccg agtgcgtgct ggccacggtg cacgggaagg
     2401 agccgccggc gtgggcgcgc gacgcgctcg aggagctgcc ggagctgctt cggtcgtcca
     2461 tggcccgcgc caacgagctc gaccgggcgg tcgtggacca cctggaggcc ctggtgctgt
     2521 cgtcgcacgt cggtgagcgc ttccgggccg tcgtggtcga tcagcgcaag ggcgcagccg
     2581 tcgtgcagct gcgcgagccg gcggtggtgg ccacggtccc gggcgagcac cccctcggcg
     2641 aggaggtcga cgtcgaggtg gtgtccgccg atccggtcgc ccggacggtc gagctggcgg
     2701 tgcggggctc gcgctagccg atggtggcgc cgggcgatcc gaaccgggcc aggacctcgt
     2761 cggcaccggg ttgcaccaga tcgaggaccg ccatgccgct gctggtcgtg cggttcgtcg
     2821 tcggcagcga cgtgggctcg agccccatgc cccggagccg cagggcgagg cggacggcgt
     2881 cgaggtagcc catctggtcg tcgatgcgca tgccgtcggc catcgaggag gtcatccggg
     2941 cgaggcggag cgggttgcgc tccccgctca tctccccgac gaccgcggac aggaactgct
     3001 gctggcggac ggtgcgcccg atgtcgccgg tgggttcggt gacctgccgg ccgtcgatgg
     3061 tctcggtgta gtagcgggag cgcacgtagg cgagtgcctg cgtgccgtcg aggacctggg
     3121 gtccggcctc gggcacgttc agccccgaca tgtggtccga cgccgggtgg gggaagtcga
     3181 tggtgatccc gccgagcgcg tcgaccaggt cccggaaggc gacgaagtcg atctccaggt
     3241 agtggtggat gggcagctgc agctggtccc gcagggtggc gatcagccgg cggggaccgc
     3301 cgttgaacgc cgagttgatg cgggtggtct gcccggtctc ggagatggtg acgagcaggt
     3361 cccggggcac ggagagcatg cgggagccct cgccgtcgac ccgcaggatc atgatcgtgt
     3421 cggagcgctc gggttcgccg ccctgggcgt cgggaccgag gatggctccg gcgttggggt
     3481 cgtcggcgga gatccccgag cgggtgtcgg agccgacgat gaggtagttg gtgccgtcgg
     3541 cggaggcgag gacgtcgccc agctccactc gctcgatgga ctggaactgc tggtagccgt
     3601 acaggaggcc gccgacggcg aacagcgcca ggaccagcac gaccccgacg agggtgcgca
     3661 ccaccgtgcg acgggacggc ggccaccggc gatcggcggg ccgcttcggc tggcggtcgc
     3721 gtgccgcagg cgtcgacttg gcgctggctg ccttcgccgc agcgggcgcg gctgccttgg
     3781 gcgcggcgcc cggggcgggg acgaccacgc gggcgggtcg gtcggcggac ggggccggtg
     3841 tggacggcgc cggagcgacc atggtccgtc cgcccacggg gtcggcgggg gcagaggacg
     3901 gcagcacgac ctcgggggtg cggcgcgggg gcggcgtcga gcgccccccg gccggagggg
     3961 cggggatgac gaagcgccgg gtgtcggcag tcgccccagg cggtgtgaca ccacgactgc
     4021 cagttcgagg aggggtcggc tcggacgcgt cggatccgcc ggtgaagcgg cgcttcccgg
     4081 ggtcccagcc cacgggggcg tcggcgatgt cgggcacccg gtcgaggtcc gggcgccggt
     4141 cgtcagccac ggacgggccc ggcggaggtg gcgcgcagcg gtcggagcac ggcgcgagtc
     4201 ttgcatcgcg gtgccaccac cgacgatgca cggtcgaacg ggaccgctcg gccttccggg
     4261 acctccgact ccgccgggac cgctgcgaaa tgagccgttc cgccctggcg gcgggcgccc
     4321 ccgacgggca gagtggaggt gcgggcacct gacgacagac cagagggacg ccagatgccg
     4381 atcaccgaac gactgcgcag ggcccggttg cgccgcgacg cgcgagcgcg gggcctggag
     4441 gtcgcactcg gtctcggtga cgagccgccg gccgaacgtc gcccggcatc cctcgtcgac
     4501 ctgcgctgcg cccgctgcgg tggcgccgga cggctcgagt ccctcgacct gcgcacgtcg
     4561 cgcggcctcg ggcggtgccc gtcctgcggg acgacctggt cgatcatcta cccgccgggc
     4621 tgagctaccc gaggagggcg gcgagctcgg cgtcggctgc ggcgacggcg ctgcgccggg
     4681 aacccgggac cgggaggtcg agctcggagg tggacgcggc gcggacggcc acgacctcgt
     4741 ccatggcctc gaccacgtcg ggggtgagga accggctgcg ctgcccggtg gagtgggcgt
     4801 cgtcgaggcg gttgcggtgg tggtggaagc gcagcggcac gtggacgtgc agctcgtccc
     4861 gggcccgggt gaccgcgacg tagagcaggc gtcgctcctc ctcgagctgc tccgggtcgc
     4921 cggtggacag atccgagggc aggttgccgt cggcggcgtg gatgaggtgg acgatgtccc
     4981 actcgccgcc cttggccgag tggatggtgg acagggtgag gacgtcgtcg tcgaggtgcg
     5041 ggggaccggc gaggtcgccg gtggacgccg gcgggtcgag ggtgagctcg gtgaggaagg
     5101 tctcgaggtc gggggtgccg gcggccagct cggccaggcg ggccaggtcg gctcgccgcg
     5161 ccgcgccgtc gccgaaccgg tcgaggtggg gatcgagcca cgcccggcac gcgtcgatgc
     5221 gctcgccggt ggccaggtcg ccgtcggctg cggtggagat cgcggaggcg agaccggcga
     5281 cctcgtcgac cgagcggggc gggacgacgc cggagtcgac gacccgggcg agggagccgt
     5341 cggcggcgtc gaggccggtg accacgcgcc gggcgatgcc ggggccgacg ccgtcgatgc
     5401 gctgcaggat gcggaaccag gccaggtcgt cccgcgggtt gaccgcgacc cggcacatgg
     5461 cgagcaggtc cttgacgtgg gcggcctcga ggaacttgag cccaccgaac ttcacgaacg
     5521 ggatccgccg cacggtgagc tcgacctcga ggaggtcggc gtggtggccg gtgcggaaca
     5581 ggacggcctg gcgtcgcagc gggatgccgc gctcgaccga ctcgagcagg cgctcgcaca
     5641 cgccgcgggc ctcggcgtgc tcgtcgccgg cggtgaccag ggccggacgg gggccggggg
     5701 cgcgggtggc gaccagctcc ttgtcgtggc gctcggtggc cgccgcccag atgcggttgg
     5761 ccacggtgac gacctcgggc gtcgagcggt ggctgcgggt gagggccacg acctcggcgt
     5821 cgaagcggga cgggaagtcg aggatgttgc gcacggtgcc gccgcggaag ccgtagatgg
     5881 ccggcgcgtc gtcgcccacc gcggtgacgg tggctccggc atcggccatg gcggcgagca
     5941 cgtcggcctg gagggcgttg gtgtcctggt actcgtcgac cagcacgtgg tcgaagcggc
     6001 cggccaccat cggcccggcc tcgggatcct ggaccagcgc ccgccacagc gaga
//
LOCUS       DATISU010000302         4800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_811392, whole genome shotgun sequence.
ACCESSION   DATISU010000302 DATISU010000000
VERSION     DATISU010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811392"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..752)
                     /locus_tag="VLR27_12070"
     CDS             complement(294..752)
                     /locus_tag="VLR27_12070"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04235.1"
                     /translation="MSTSAAPVHAASQFRGRGEYAIDDKGRLTLPVQMRKALLGGGSL
                     VVLDGRAVIWDEPTYRDAVDQLNERVTTGELTSAQVRGFLSSTHLVSPDSQGRIVIPH
                     AVRIEAGLERDVLVLGAGPRIEIVPVGDAVLDAGLTVDDAVVEVLDHARF"
     gene            complement(1033..1689)
                     /locus_tag="VLR27_12075"
     CDS             complement(1033..1689)
                     /locus_tag="VLR27_12075"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04236.1"
                     /translation="MSDWAVEHHRGSAAELHALEVSDDGTPRIWVLAATGPAVVLGST
                     QPDDVVDAEAARAAGVAVARRRSGGGAVWVAPGDPHWIDVIVPRGHELWTDDVGRAFL
                     PIGRAWQEALASLGVEGTHLHEDRLACGPLGGTVCFAGRGPGEVLLGEAKVVGISQRR
                     TRAGARFQCAVPVHWDPAPLAALLRDHPDPDALAGVGTGIGESITSAQLVEALITALP
                     "
     gene            complement(1686..2198)
                     /locus_tag="VLR27_12080"
     CDS             complement(1686..2198)
                     /locus_tag="VLR27_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HSP04237.1"
                     /translation="MSGRALVLNASYEPLSVVSSRRATVLMLAEKADLVSPSGLCFHS
                     EHLAVEVPSVVRLRHFVKVPFARRAALNRRAVFARDGGRCQYCGDSADSIDHVVPRAK
                     GGPHTWENVVAACRPCNVRKRDRLLHDTSMSLRSTPRAPAGHSWVLVSVGAVPDDWHP
                     YLALEPALSA"
     gene            complement(2195..2506)
                     /locus_tag="VLR27_12085"
     CDS             complement(2195..2506)
                     /locus_tag="VLR27_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04238.1"
                     /translation="MEGPAAAQLTGAGRRPSGTGAVAVAIARRPRLWPAAARQARALV
                     PSRWWRRRPFLPVPDRAWLRFRMTTAYGDAGAALDVEDLLTWLAWTDTVRRGTGGAER
                     R"
     gene            complement(2479..3738)
                     /locus_tag="VLR27_12090"
     CDS             complement(2479..3738)
                     /locus_tag="VLR27_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP04239.1"
                     /translation="MQPTWGTEAEQYREKVQAFLAEHLPADWRGIGALPRDEVHDWTE
                     RWRQTLFDNNLLAPRWPTEYGGPGLTETETVVLAEEFAKAGVPTGGPNDAFSIQMVGN
                     TILQWGTEEQKREFIPKILSGEHTWCQGYSEPNAGSDLGNLGCRATLDGEEWVINGQK
                     IWTSAGHLADWIFVLARTDVDAPKHRGITFLLVPMDQPGVEVRPIEMINGDSEFNEVF
                     FSDARCAKENVIGGVNNGWAVAMTLLGYERGDAAATFPVSFRIELDRLLALARDRGLD
                     QDPVIRQRLAQAHSTVEIMKWSGWRTLTAHLSGAHPGPEAGLFKLQWSEYHRRVTELA
                     VDILGADALAPSGRAPASSFQTDDPGAPNDSASWVGTFLTARAGTIYAGSSEIQRNIV
                     GEMVLGLPKEPRPEGAGASWKAQQQRS"
     gene            complement(3781..4665)
                     /locus_tag="VLR27_12095"
     CDS             complement(3781..4665)
                     /locus_tag="VLR27_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSP04240.1"
                     /translation="MATLAIDIGGTKLAAGVVDAVGSLAHRAQRATAAIGDGEALFAD
                     LIGLIDGVLHDARAAGIDIEACGVGCGGPMTAGGAEVSPLNIPAWRRFPLRARLAEHL
                     DLGVAVDNDAKALALGEGWCGAAAGERDFLAMVVSTGVGGGIVSRGRLLEGASGNAGH
                     VGHVFVADGTEAWPRHPAGLLESEASGTAIAQRTGAPAADAGVDVRARTGTLVGRAVA
                     SVANLLDLRLAVVAGSVALGYGEPFFAAAQAEVDRLCHLDFSAGTRVIPAGLGDAGPL
                     IGAAAVSLRARGVDIGIV"
BASE COUNT          643 a         1683 c         1851 g          623 t
ORIGIN      
        1 cgggaccggg gcgaaggcgg cgacgacctc gtcccgcagg acggggacgt ggcggaaggt
       61 ccgggtggag tcgtctgcgt cgctcaccgg cagcccctcg gccgccgaag gcccgccggg
      121 atgtatgccc cggtcgggca cgatgaaaga gcgggcggag agtaggggcc ttccatctcg
      181 acgcccgaga ggctgcgagt gagcggcgcg tggactcctg ttgtggtggt gctcgtctgc
      241 tcggtggacg cgctcccttc gttcacggtc ggtggtctgt ggtgggtggg atgtcagaag
      301 cgcgcatggt cgaggacctc gacgaccgcg tcgtccacgg tcaggccggc gtcgagcacg
      361 gcgtcgccga cggggacgat ctcgatgcgg gggccggcac cgagcacgag cacgtcgcgc
      421 tccaggccgg cctcgatgcg cacggcgtgc gggatcacga tgcggccctg ggagtccggc
      481 gagacgaggt gggtgctcga caggaacccg cggacctggg cgctggtgag ctcgccggtg
      541 gtgacccgct cgttcagctg gtcgacggcg tcgcggtagg tgggctcgtc ccagatgacg
      601 gcccggccgt cgaggacgac cagggagccg ccgccgagga gggccttgcg catctggacc
      661 gggagggtca ggcggccctt gtcgtcgatg gcgtactcgc cccggccccg gaactgggag
      721 gcggcgtgga ccggagcagc ggacgtggac acatcagggc tcatcagggc tgtcgcccca
      781 ctgcgctccg ttgtcgtcgc tccggtctcc gccatgcagg gcacagtacg tccatctagg
      841 gaagaacgtc aacgtctcag gccacattcc ccacccaatg tcgcgatcgg tgtcacgacc
      901 ggggcgtacc agggcgtggc ctccactccc atcccctggc gactggtgtg gccgcctgct
      961 gtgcgccgac tctggaagct ggcagtgatc tcgtcgcacg gaagcccgtg tgcgacgacc
     1021 tcactgccgg tttcagggga gggcggtgat gagggcttcg accagctggg cgctggtgat
     1081 cgactcgccg atgccggtgc cgacgccggc cagggcgtcg gggtccgggt ggtcgcgcag
     1141 cagcgctgcc aagggagccg ggtcccagtg caccggcacc gcgcactgga agcgggcgcc
     1201 ggcgcgggtg cggcgctggc tgatcccgac gaccttggcc tcgccgagga gcacctcgcc
     1261 ggggccgcgg ccggcgaagc agacggtgcc gccgagcggg ccacaggcga ggcggtcctc
     1321 gtggaggtgg gtgccctcga cgccgagcga cgccagtgcc tcctgccagg cccgcccgat
     1381 gggcaggaac gcccggccga cgtcgtcggt ccacagctcg tggccacggg ggacgatcac
     1441 gtcgatccag tgggggtccc cgggcgcgac ccacaccgcg cccccgccgc tgcgccggcg
     1501 ggccacggcc acgccggcgg cgcgcgccgc ctcggcgtcg acgacgtcgt cgggctgggt
     1561 cgagcccagc accacggcgg gaccggtggc cgccagcacc cagatgcgcg gggtgccgtc
     1621 gtcggagacc tcgagggcgt ggagctcggc ggcggatccc cggtggtgct cgacggccca
     1681 gtcgctcacg cggacaacgc cggctcgagc gcgaggtacg ggtgccagtc atcgggcacc
     1741 gcccccaccg acaccagcac ccacgagtgg ccggcgggcg cccggggcgt ggaccgcagc
     1801 gacatcgagg tgtcatgcag gagccggtcc cgcttgcgca cgttgcacgg ccggcacgca
     1861 gccaccacgt tctcccaggt gtgcgggccg cccttggcgc ggggcacgac gtggtcgatg
     1921 gagtcggcgg agtccccgca gtactggcag cggccaccgt cgcgggcgaa cacggcccgc
     1981 cggttgaggg cggcgcggcg agcgaagggg accttgacga agtggcgcag gcgcacgacc
     2041 gacggcacct cgacggcgag gtgctcggag tggaagcaga ggcccgacgg cgacacgaga
     2101 tcggccttct cggccagcat gagcacggtg gcccgcctcg acgagaccac cgacagcggc
     2161 tcgtaggagg cgttcagcac cagtgcccgg ccgctcatcg ccgctcggcc cctccggtcc
     2221 ctcttctcac cgtgtcggtc caggccaacc aggtgagcag gtcctcgacg tcgagggccg
     2281 cgccggcgtc gccgtaggcc gtggtcatcc ggaaccggag ccaggcccgg tcgggcacgg
     2341 ggaggaacgg gcggcggcgc caccacctgg acggcaccag cgcgcgggcc tggcgggcgg
     2401 ccgcgggcca caggcgcggc cggcgggcga tggcgaccgc gacggcaccc gtccctgacg
     2461 gacggcggcc ggccccggtc agctgcgctg ctgctgggcc ttccacgagg ctcctgcccc
     2521 ttcgggtcgg ggctccttgg ggaggccgag gaccatctcg ccgacgatgt tgcgctggat
     2581 ctcgctggag ccggcgtaga tcgtgccggc ccgggcggtg aggaacgtgc cgacccagga
     2641 cgccgagtcg ttgggggcgc cggggtcgtc ggtctggaag ctcgaggccg gtgcccgtcc
     2701 ggacggtgcc agcgcgtcgg cgccgaggat gtcgacggcg agctcggtga cccggcggtg
     2761 gtactcggac cactgcagct tgaacaggcc ggcctccggg ccggggtggg cgccggagag
     2821 gtgggcggtg agggtgcgcc agccggacca cttcatgatc tcgaccgtgc tgtgcgcctg
     2881 ggcgagccgc tggcggatga ccgggtcctg gtcgagcccg cggtcgcggg ccagcgccag
     2941 gaggcgatcg agctcgatgc ggaacgagac cgggaacgtg gcagccgcgt cgccgcgctc
     3001 gtagccgagc agggtcatgg ccaccgccca gccgttgttc acgccgccga tcacgttctc
     3061 cttggcgcag cgggcgtcgg agaagaagac ctcgttgaac tcggagtcgc cgttgatcat
     3121 ctcgatcggg cggacctcga cgccgggttg gtccatgggg accagcagga acgtgatgcc
     3181 ccggtgcttg ggggcgtcga cgtcggtgcg ggcgagcacg aagatccagt cggcgaggtg
     3241 cccggccgag gtccagatct tctggccgtt gatcacccac tcctcgccgt cgagggtggc
     3301 ccggcagccg aggttgccga ggtccgagcc ggcgttgggc tcggagtagc cctggcacca
     3361 cgtgtgctcg ccactgagga tcttcgggat gaactcccgc ttctgctcct cggtgcccca
     3421 ctgcaggatg gtgttgccga ccatctggat gctgaacgcg tcgttcggcc cgccggtggg
     3481 cacgccggcc ttggcgaact cctcggccag cacgacggtc tcggtctcgg tcaggccggg
     3541 gccgccgtac tcggtgggcc agcggggcgc gagcaggttg ttgtcgaaca gcgtctggcg
     3601 ccaccgctcg gtccagtcgt ggacctcgtc gcggggcagc gcgccgatgc ccctccagtc
     3661 ggccgggagg tgctcggcga ggaaggcctg gaccttctcg cggtactgct cggcctcggt
     3721 cccccacgtg ggctgcatag ccgagagtct gccacgacca ccccgacccc ggacgggcgg
     3781 tcagacgatc ccgatgtcca cgccccgggc ccgcagcgac accgccgcgg ccccgatgag
     3841 cggtccggcg tcgccgaggc cggcggggat gacgcgggtt ccggcggaga agtcgaggtg
     3901 gcagaggcgg tcgacctcgg cctgggcggc ggcgaagaac ggctcgccgt agccgagggc
     3961 gaccgacccg gcgaccacgg ccaggcgcag gtcgagcagg ttggccaccg acgcgaccgc
     4021 ccgacccacc agcgtgcccg tccgggcccg gacgtcgaca ccggcgtcgg cggccggggc
     4081 gccggtgcgc tgggcgatgg ccgtgccgct ggcctcggac tcgagcagac cggcggggtg
     4141 ccgcggccag gcctcggtgc cgtcggcgac gaagacgtgc ccgacgtggc cggcgttgcc
     4201 cgacgcgccc tcgagcaacc gtccccggct cacgatcccc ccgcccacgc cggtggacac
     4261 caccatggcg aggaagtccc gctcccccgc cgcggcgccg caccagccct ccccgagggc
     4321 gagcgccttg gcgtcgttgt ccacggcgac accgaggtcg aggtgctcgg ccaggcgggc
     4381 ccggaggggg aaccggcgcc aggccgggat gttgaggggc gagacctctg caccgcctgc
     4441 cgtcatcggt ccgccgcagc ccaccccgca cgcctcgatg tcgatgccag cggcgcgggc
     4501 gtcgtgcagc acgccgtcga tcaacccgat gaggtcggcg aagagcgcct cgccgtctcc
     4561 gatcgcggcg gtggcgcgct gggcccggtg cgccagggag ccgacggcgt cgacgacgcc
     4621 tgccgccagc ttcgtgccgc cgatgtcgat cgcgagggtc gccacccggg cgatggtagg
     4681 gagcccggca cctcgacccc gagcatccgc tcggcacccc ggcgccgtag tctgcccagg
     4741 cggcggggcg caccgatgtc ggccgtgcca cgacgacgca gacgaccagg aggacgggtg
//
LOCUS       DATISU010000303         5140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_532472, whole genome shotgun sequence.
ACCESSION   DATISU010000303 DATISU010000000
VERSION     DATISU010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532472"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1936)
                     /locus_tag="VLR27_12100"
     CDS             complement(<1..1936)
                     /locus_tag="VLR27_12100"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSP04241.1"
                     /translation="MNRESQEPGALHAVDARALPALFDTVLDQSATGVMVFDEELRLA
                     YVNEVAARIGGYPAEVHIGKRLTDLYPQIAVQADPLMARALAEQETMRNEELVWESPR
                     PPHERRYWQVTYVPVRSTADQPYVAAIYVETTPVRRAHERLTRLLDALPIFTGMCTPD
                     GNLLEANEATLAATELSREDVVGFPLWEASWWRDDPAVQVTLREMLTRAQAGEPTQED
                     IEVHLEDGRRVTIDFQLVPIVEHGAVTALVPSGVDITGRIVERDRLQALASLSRHLNG
                     ALMTAQVARLVVANAPAVLDAEFVNVALLDADRQELRLIQPLMDPDIEERWGTLPLDG
                     TRTPFHDVVRSGAPVLVGRPEREARYPEMVRDTERIGLDSTASVPLVDELGDVFGVLG
                     VGWSEAIEFTEEIRLRLDLLADLCSHALRRAQRTEARDRLVEEMQVEVLAMPDTSRTL
                     DVSLAYEPARGDIGFGGDWYDVIEINESCTALVVGDVAGHGITAAARMTEAKATIRTL
                     VLNVDHAEVIPAANRSLAHFDSGYIATAAVAWIDDEAKTLEWRLAGHLPPVLRTTDGD
                     AYLLEGVQHPPIGTATMPRVQESIPFPQGSLLVLYTDGLVERRGEDIDVGLERLRSIV
                     DALPPTCSAREARDSIMAELQ"
     gene            complement(2075..2728)
                     /locus_tag="VLR27_12105"
     CDS             complement(2075..2728)
                     /locus_tag="VLR27_12105"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HSP04242.1"
                     /translation="MDTVSGIGVLDKVVAILRVVGEGPATFRELCERTEISRATAHRL
                     AQGMEVVGLLRRGDDGAYTPGLALIGLGRAALEAFPLTTVAAPVLRRLRDDTGESVQL
                     FVVEEGERLCVAAIDSGHELRTIVPVGARLPMDVGSAGRVLGGGVEPGGWVESVGERA
                     PGVASVSAPVRDGDGRTLAAISVSGPIERVSRQPGARFGASVVAAAGEVEAALRHRL"
     gene            2777..4177
                     /gene="leuC"
                     /locus_tag="VLR27_12110"
     CDS             2777..4177
                     /gene="leuC"
                     /locus_tag="VLR27_12110"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HSP04243.1"
                     /translation="MAGKTLSEKVWDRHLVHSGDDGDLLYIDLHLVHEVTSPQAFDGL
                     RLAGRTVRRPDLTLATMDHNVPTANIDQPVADPISKKQMDTLQANCDEFGITCHAMGD
                     PGQGIVHIIGPEQGLTQPGMTIVCGDSHTSTHGAFGAIAFGIGTSEVEHVLATQTLPQ
                     SRPRTMAVTVEGDLQPGVTAKDVVLAILGRIGTGGGIGHMIEYRGSAIRSLSMEGRMT
                     VCNMSIEMGAKAGLIAPDETTFEYLQGRPHAPAGADWDAAVADWRTLVTDDDATFDKE
                     VVIDAAEITPHVSWGTNPGQVVPITGEVPHPDAMVDATERNAAERALDYMGLAAGTPM
                     KQVPVDTVFIGSCTNGRIEDLRAVAEVAKGRKVAINRAMIVPGSFKVKEQAVAEGLDK
                     IFDEAGFDWREPGCSMCLAMNPDKLAPGERCASTSNRNFEGRQGRGGRTHLVSPAVAA
                     ATAIAGHFATPADLEN"
     gene            4181..4768
                     /gene="leuD"
                     /locus_tag="VLR27_12115"
     CDS             4181..4768
                     /gene="leuD"
                     /locus_tag="VLR27_12115"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HSP04244.1"
                     /translation="MRAVRTIEGTAVPLDRSDVDTDQIIPSDWLKQVERTGFEKGLFS
                     EWRDDPGFVLNLEQYQGANVLVGGANFGTGSSREHAVWAIQQYGFEAVISPRFGDIFR
                     NNCTKNGLVPVQVDAETGERLMRMVEDDPTTVFEIDIASRTLRAGDVETTFPLDEGTQ
                     HRFLEGLDDIGLTLKLGDEISDFETKRPHHLPTAS"
     gene            complement(4805..>5140)
                     /locus_tag="VLR27_12120"
     CDS             complement(4805..>5140)
                     /locus_tag="VLR27_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HSP04245.1"
                     /translation="QVIAEGTPLESVISVGAVEQITEVLGASEVQTAGARVTATEVAL
                     LTGMQGGIVAGTGVTEARVGGSMVSAPAGPQPTLPRTGSSDDLTLLAGMGALALLGAA
                     LHLRRRATA"
BASE COUNT          782 a         1901 c         1780 g          677 t
ORIGIN      
        1 gctggagctc ggccatgatc gagtcccggg cctcacgggc cgagcacgtc ggcggcagcg
       61 cgtcgacgat cgaccgcagg cgctcgaggc cgacatcgat gtcctcgcct cgccgctcca
      121 cgagcccgtc ggtgtagagg acgagcaacg agccctgggg gaaggggatg gactcctgca
      181 cgcggggcat cgtggccgtg ccgatcggcg ggtgctggac gccctcgagc aggtaggcgt
      241 cgccatcggt cgtgcggagg acgggcggca ggtggccggc gaggcgccac tcgagggtct
      301 tggcctcgtc gtcgatccac gccacggcgg ccgtggcgat gtagccggag tcgaagtgcg
      361 cgagcgaccg gttcgccgcc gggatcacct cggcgtggtc cacgttcagg accagggtcc
      421 ggatcgtggc cttggcctcg gtcatgcgcg ccgccgcggt gatgccgtgg ccggcgacgt
      481 caccgaccac gagcgccgtg cacgactcgt tgatctcgat gacgtcgtac cagtcgccac
      541 cgaagccgat gtcaccccgg gccggttcgt acgccaggga cacgtcgagc gtccgggagg
      601 tgtcgggcat cgccagcacc tcgacctgca tctcctcgac caggcgatcc cgagcctcgg
      661 tccgctgcgc ccgccggagc gcgtggctgc agaggtcggc cagcaggtcg agacgcagcc
      721 ggatctcctc ggtgaactcg atcgcctcgc tccacccgac accgagcacg ccgaacacgt
      781 cgccgagctc gtcgaccagg gggaccgacg cggtgctgtc cagaccgatc cgctcggtgt
      841 cccggaccat ctcggggtac cgggcctccc gttccggccg gccgaccagc accggcgcac
      901 cggaccgcac cacgtcgtgg aacggcgtgc gcgtcccgtc gagcggcagc gtcccccacc
      961 gctcctcgat gtcggggtcc atgaggggct ggatgagccg gagctcctgg cggtcggcgt
     1021 cgagcagggc gacgttgacg aactccgcgt cgaggaccgc aggcgcgttg gccacgacca
     1081 gccgagcgac ctgggcagtc atgagcgccc cgttgaggtg gcgggacagc gatgccaggg
     1141 cctgcaggcg gtcgcgctcg acgatccgcc cggtgatgtc gacacccgag gggaccaggg
     1201 ccgtgacggc accatgctcc acgatcggga cgagctggaa gtcgatggtg acccgacgcc
     1261 cgtcctccag gtggacctcg atgtcctcct gcgttggctc gccggcctgg gcccgggtca
     1321 gcatctcgcg cagcgtcacc tgcacggcgg gatcgtcgcg ccaccaggac gcctcccaca
     1381 gcgggaagcc gacgacgtcc tcgcgggaca gctcggtggc ggcaagggtc gcctcgttcg
     1441 cttcgagcag gttgccgtcc ggcgtgcaca tgccggtgaa gatcggaagg gcatcgagca
     1501 gccgggtcaa gcgctcgtgg gcccggcgca ccggggtggt ctccacgtag atcgccgcca
     1561 cgtacggctg gtcggcggtc gagcgcaccg gcacgtaggt gacctgccag taccggcgct
     1621 cgtggggcgg ccgcggcgac tcccagacga gctcctcgtt gcgcatcgtc tcctgctcgg
     1681 ccagggcgcg ggccatgagc gggtcggcct gcacggcgat ctgggggtag aggtcggtga
     1741 gccgcttgcc gatgtggacc tcggccgggt agccgccgat gcgggccgcc acctcgttga
     1801 cgtaggcgag acgcagctcc tcgtcgaaga ccatcacgcc cgtggcggac tggtcgagca
     1861 ccgtgtcgaa cagcgccggg agggcccgcg cgtcgaccgc gtgcagcgct cccggctcct
     1921 ggctctcgcg gttcatgctc acggatcgct ccgggtcgcc gtcgtcaaag cacgtgcttc
     1981 gtgttcgcct gccctctcgg cagcacccgc caccgtcccg caccccgaag gtacccgtcc
     2041 cgccggtcgg ggacaggggt cagaggtcct cttctcagag gcggtgccgc agggcggcct
     2101 cgacctcgcc cgccgcggcg acgaccgacg cgccgaaccg cgcccccggc tggcggctca
     2161 cccgctcgat gggacccgag acgctgatgg cggccagggt ccggccgtcg ccgtcacgga
     2221 ccggcgccga caccgacgcc acccccgggg cccgctcacc gaccgactcc acccagccgc
     2281 ccggctcgac tccgccaccc agcacccgac cggccgagcc gacgtccatg gggagacgcg
     2341 caccgaccgg gacgatggtg cgcagctcgt ggccggagtc gatggccgcc acgcacagcc
     2401 gctccccctc ctccaccacg aacagctgga ccgactcgcc ggtgtcgtcg cgcagccgac
     2461 gcagcaccgg tgcggcgaca gtggtgaggg ggaacgcctc gagcgcggca cgcccgaggc
     2521 cgatgagggc gagcccgggc gtgtaggcgc cgtcgtcgcc ccgccggagc agcccgacga
     2581 cctccatccc ctgggcgagg cggtgcgccg tggcccggct gatctcggtc cgctcgcaca
     2641 gctcccggaa ggtcgctggc ccctcgccga ccacgcgcag gatggcaacg accttgtcga
     2701 gcacgccgat cccgcttaca gtgtccatca gacgggaaca ctatcccacc atgtgagaca
     2761 gtcaagggag cgagcgatgg caggcaagac gttgagcgag aaggtgtggg accggcacct
     2821 cgtgcactcc ggggacgacg gggacctgct ctacatcgat ctccacctcg tgcacgaggt
     2881 cacctccccc caggccttcg acgggctccg cctcgccggg cgcacggtcc gccgccccga
     2941 cctcaccctg gcgacgatgg accacaacgt gccgaccgcg aacatcgacc agccggtggc
     3001 cgaccccatc tccaagaagc agatggacac cctgcaggcg aactgcgacg agttcggcat
     3061 cacctgccac gcgatgggcg accccgggca gggcatcgtc cacatcatcg gccccgagca
     3121 gggtctcacc cagcccggca tgaccatcgt ctgcggcgac tcccacacct ccacccatgg
     3181 cgcgttcggg gcgatcgcgt tcggcatcgg cacctccgag gtcgagcacg tgctggccac
     3241 ccagacgctc ccccagtccc ggccccggac catggccgtc accgtcgaag gcgacctgca
     3301 gccgggcgtc accgccaagg acgtggtgct ggccatcctc ggccgcatcg gcaccggcgg
     3361 cggcatcggc cacatgatcg agtaccgcgg ctcggccatc cgctccctct ccatggaggg
     3421 gcgcatgacg gtgtgcaaca tgtccatcga gatgggcgcc aaggccggcc tgatcgcgcc
     3481 ggacgagacc acgttcgagt acctgcaggg ccgtccgcac gcgccggccg gcgccgactg
     3541 ggacgcggcg gtcgccgact ggcggacgct ggtcaccgac gacgacgcca ccttcgacaa
     3601 ggaggtggtg atcgacgccg ccgagatcac cccgcacgtg tcctggggca ccaaccccgg
     3661 tcaggtcgtg ccgatcaccg gcgaggtccc gcaccccgac gccatggtcg atgccacgga
     3721 gcgcaacgcc gccgagcgag ccctcgacta catgggcctc gccgccggca cgccgatgaa
     3781 gcaggtgccg gtggacacgg tgttcatcgg ctcgtgcacc aacgggcgca tcgaggacct
     3841 ccgggccgtg gccgaggtgg caaagggccg caaggtcgcc atcaaccggg cgatgatcgt
     3901 gcccggctcg ttcaaggtga aggagcaggc cgtcgccgag ggcctcgaca agatcttcga
     3961 cgaggccggc ttcgactggc gcgagccggg ctgctccatg tgcctggcca tgaaccccga
     4021 caagctcgcc cccggtgagc gctgcgccag cacctcgaac cggaacttcg agggccgcca
     4081 gggccggggc ggtcgcaccc acctcgtgtc ccccgccgtc gccgcggcca ccgccatcgc
     4141 cggccacttc gccacccccg ctgatctgga gaactgagac atgcgcgctg tccgcaccat
     4201 cgaaggcacc gcggtcccgc tcgaccgctc cgacgtcgac accgatcaga tcatcccgtc
     4261 cgactggctc aagcaggtcg agcggaccgg gttcgagaag ggcctgttct cggagtggcg
     4321 cgacgacccc ggcttcgtcc tcaacctgga gcagtaccag ggggccaacg tcctcgtcgg
     4381 cggggccaac ttcggcaccg gctcgtcccg cgagcacgcc gtctgggcca tccagcagta
     4441 cgggttcgag gcggtgatct ccccccgctt cggcgacatc ttccgcaaca actgcaccaa
     4501 gaacggcctg gtgccggtgc aggtcgacgc cgagaccggt gagcgcctca tgcgcatggt
     4561 cgaggacgac cccaccaccg tgttcgagat cgacatcgcc agccgcaccc tgcgcgccgg
     4621 cgacgtggag accacgttcc ccctcgacga gggcacccag caccggttcc tcgagggcct
     4681 cgacgacatc ggcctcaccc tgaagctggg cgacgagatc agcgacttcg agacgaagcg
     4741 accccaccac ctccccacgg cctcgtaggc ccccgagaac ggttccgggc ggtgggctgg
     4801 tcggtcaggc ggtggcgcgg cggcgcaggt gcagcgcagc cccgagcagg gcgagcgcac
     4861 ccatgccggc gagcagcgtg aggtcgtcgg acgagccggt gcggggcagg gtcggctgcg
     4921 gaccagccgg cgcgctgacc atgctgccgc cgacgcgggc ctcggtgacg ccggtgccgg
     4981 cgacgatgcc gccctgcatg ccggtgagga gggcgacctc ggtggcggtc acccgggcgc
     5041 cggcggtctg gacctcggag gcgccgagca cctcggtgat ctgctcgact gcgcccacgc
     5101 tgatgaccga ctcgaggggc gtgccctcgg cgatgacctg
//
LOCUS       DATISU010000304         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_486433, whole genome shotgun sequence.
ACCESSION   DATISU010000304 DATISU010000000
VERSION     DATISU010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486433"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VLR27_12125"
     CDS             <1..471
                     /locus_tag="VLR27_12125"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HSP04246.1"
                     /translation="QVERAILWGFDVSHLDSHMGVMQLRPEFFDVYLDLAVDYGLPIR
                     LSGASTQRAIGFPFRDLAAEEGVLFPDRFLYAPGIGSRKAIERTIYDLQPGVTEVYAH
                     PAVDTPELRAIGDTWSGRVDDLDLVLSSETRSLLERAGVTLIGYRELRDAQRAG"
     gene            474..860
                     /locus_tag="VLR27_12130"
     CDS             474..860
                     /locus_tag="VLR27_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04247.1"
                     /translation="MRPNIRRAVVIAIAGAALLSGACDSDRRAISRDDLPDVPSEPDH
                     VVELGDDGFDTDTLEVLNSDLVEFRIVGEDDHGIRADTRIDTGLLFPGESTFVIFDQP
                     GDYEVVDVTDEDQVLQVESVQDPDEE"
     gene            complement(868..1266)
                     /locus_tag="VLR27_12135"
     CDS             complement(868..1266)
                     /locus_tag="VLR27_12135"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HSP04248.1"
                     /translation="MGEPVELLTQAWLDAQRELTQRLPERPGASARMEYRVSGGPDGE
                     VVFHTVLEDGRIAESALGPATPEPDFTVLVPHAEYVAIVRGELDQNVGFMQGRVKVTG
                     NIGRMLSVLPVTTSPEWRAAADDLLARTTV"
     gene            complement(1266..2147)
                     /gene="mca"
                     /locus_tag="VLR27_12140"
     CDS             complement(1266..2147)
                     /gene="mca"
                     /locus_tag="VLR27_12140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HSP04249.1"
                     /translation="MSPNPARCILTVHAHPDDESSKGAATIARCQAEGIRTVLVCCTD
                     GAAGDILNPAMDREDVKANLPAVRREELARAAEIIGYDEVIDLGYRDSGMPDTDANAH
                     PEAFANADHDEAVGKLVEILRRERPQVVVTYGDDQRGYPHPDHLQVHDISMPAVERAA
                     DPEWYPEAGEPHRVDKVYWTSWSRGRMVAMHEKFLEMGLESPFDEKWFDRPDQDERIT
                     TRVHTGEWSDVRWDALLAHATQIDPTSPFWFGLPREVAATVHPWDEYILAVSHVDSDE
                     STGDLFDGIPVRDAASR"
     gene            complement(2158..2463)
                     /locus_tag="VLR27_12145"
     CDS             complement(2158..2463)
                     /locus_tag="VLR27_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04250.1"
                     /translation="MATPPATEPTQRSLGRSVALGIAVGVGVGAICFFLIAIPFYTLA
                     SFEPNGIDRPVVRTGLFKVALPVGLLVGLASGVAAGLWLRRGGTWTVNDGSARYRSR"
     gene            2500..3228
                     /locus_tag="VLR27_12150"
     CDS             2500..3228
                     /locus_tag="VLR27_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04251.1"
                     /translation="MPSRPVLDRQRWAGVLVALTLAGGVSACSDEPDEIAATGDERAA
                     QAEEAARDAGLDAEVADFLGLLARGETATYRVRFPGPSEGAELEVTSRPPDRRVELVA
                     EGTTTEVRLITGGEAFTCTPHDGDELACDRTDVLVEPPGVFSAGALDELSGSLAERAD
                     DYTFEIEVLPVAGVEAQCLVTRLRSGREAPELGASGTICASAEGAVLLVDQGSERLEA
                     TEYSTDVGDDAFVRPDAPDGGTGG"
BASE COUNT          468 a         1175 c         1185 g          456 t
ORIGIN      
        1 caggtcgagc gggcgatcct gtggggcttc gacgtcagcc acctcgacag ccacatgggc
       61 gtcatgcagc tgcggcccga gttcttcgac gtctacctcg acctggccgt cgactacggc
      121 ctgcccatcc gcctctcggg agcgtccacc cagcgggcca tcggcttccc gttccgcgac
      181 ctggccgccg aggagggtgt gctcttcccg gaccggttcc tgtacgcccc gggcatcggg
      241 tcccggaagg ccatcgagcg gaccatctac gacctgcagc ccggcgtcac cgaggtctac
      301 gcgcaccccg ccgtcgacac gcccgagctg cgagccatcg gcgacacgtg gtccggtcgg
      361 gtcgacgacc tcgacctggt gctcagctcc gagaccaggt ccctgctcga gcgcgccggc
      421 gtcaccctca tcggctaccg ggagctgcgc gacgcgcagc gggccggctg agcgtgcgcc
      481 cgaacatccg gcgagccgtc gtcatcgcca tcgccggggc cgcgctgctg agcggcgcct
      541 gcgacagcga ccgccgcgcg atctcacggg acgacctccc cgacgtgccc tccgaaccag
      601 accacgtcgt ggagctgggt gacgacggct tcgacacgga caccctcgag gtgctgaaca
      661 gcgacctggt cgagttccgc atcgtcggtg aggacgacca cggcatccgc gccgacacgc
      721 gcatcgacac cgggctgctg ttccccggcg agtccacgtt cgtcatcttc gaccagcccg
      781 gcgactacga ggtcgtcgac gtcaccgacg aggaccaggt gctgcaggtg gagtcggtcc
      841 aggaccccga cgaggagtag ccggacctca gaccgtggtg cgggcgagca ggtcgtcggc
      901 cgccgcccgc cactccgggg aggtggtcac cggcagcacc gagagcatcc gtccgatgtt
      961 cccggtcacc ttgacccggc cctgcatgaa gccgacgttc tggtccagct cgccccgcac
     1021 gatggccacg tactcggcgt gggggacgag caccgtgaag tcgggctccg gggtcgccgg
     1081 accgagcgcg ctctcggcga tgcgtccgtc ctcgaggacg gtgtggaaca cgacctcgcc
     1141 atcggggccg ccggacaccc ggtactccat ccgggcgctg gccccgggcc gctccggcag
     1201 gcgctgggtg agctcgcgct gggcgtcgag ccaggcctgg gtgagcagct cgaccggctc
     1261 gcccatcagc gggaggcggc gtcgcgcacc gggatgccgt cgaagaggtc gccggtggac
     1321 tcgtcgctgt ccacgtggct caccgccagg atgtactcgt cccacgggtg cacggtggcg
     1381 gcgacctcgc ggggcagacc gaaccagaac ggcgaggtcg ggtcgatctg ggtggcgtgg
     1441 gcgagcaggg cgtcccagcg cacgtcggac cactcccccg tgtgcacgcg cgtggtgatt
     1501 cgctcgtcct ggtcggggcg gtcgaaccac ttctcgtcga agggcgattc gaggcccatc
     1561 tcgaggaact tctcgtgcat ggcgaccatg cgaccccgcg accaggaggt ccagtacacc
     1621 ttgtcgacgc ggtggggctc gcccgcctcg gggtaccact cgggatccgc cgcccgctcc
     1681 accgccggca tcgagatgtc gtgcacctgc aggtggtcgg ggtgggggta gccgcgctgg
     1741 tcgtcgccgt aggtgaccac gacctgcggg cgctcgcgac gcaggatctc gacgagcttg
     1801 ccgaccgcct cgtcgtggtc ggcgttggcg aaggcctcgg gatgggcgtt ggcgtcggtg
     1861 tcgggcatgc cggagtcgcg gtacccgagg tcgatcacct cgtcgtagcc gatgatctcg
     1921 gcagcccgcg ccagctcctc acggcgcacg gcggggaggt tcgccttgac gtcctcgcgg
     1981 tccatcgccg ggttgaggat gtccccggcg gcgccgtcgg tgcagcagac gagcaccgtc
     2041 cggatgccct ccgcctggca ccgggcgatc gtcgcggcac ccttcgacga ctcgtcgtcg
     2101 gggtgggcgt ggacggtcag gatgcaacgg gcagggttcg ggctcacccc ggcaacgcta
     2161 acggctgcgg tagcgggcgc tcccgtcgtt gacggtccac gtcccgccac gtcgcaacca
     2221 cagcccggcc gcgacgcccg acgccaaccc cacgaggagc ccgacgggca gcgcgacctt
     2281 gaacaggcca gtgcgcacga cgggccggtc gattccgttc ggctcgaacg acgcgagcgt
     2341 gtagaagggg atcgcgatca ggaagaagca gatggcgccg acgccgacgc cgactgcgat
     2401 gcccagcgcg acggagcggc cgagcgaccg ttgcgtgggt tcggtcgcgg gaggcgttgc
     2461 cattccccca acggtactgc gggtccctac catccgaacg tgcccagccg acccgtgctc
     2521 gatcggcagc gttgggcggg cgtgctggtt gcgctgacgc tcgccggtgg cgtctcggcc
     2581 tgcagcgacg agcccgacga gatcgccgcc acgggcgacg agcgcgccgc gcaggccgag
     2641 gaggcggccc gggatgctgg actcgacgcc gaggtggcgg acttcctcgg gctgctcgcc
     2701 cgaggcgaga cggcgaccta ccgcgttcgc ttccccggcc cgtccgaggg cgcggagctg
     2761 gaggtgacga gccgtccgcc cgatcgccgg gtcgagctcg tcgccgaggg cacgaccacc
     2821 gaggtccggc tcatcacggg cggggaggcc ttcacgtgca ccccgcacga cggagacgag
     2881 ctcgcatgcg accggaccga cgtcctggtc gagccgcccg gcgtgttcag tgcgggcgcc
     2941 ctcgacgagc tgagcgggtc cctggccgag cgcgccgacg actacacctt cgagatcgag
     3001 gtcctccccg tcgccggcgt cgaggcgcag tgcctggtga cgcggctgcg gagcggacgg
     3061 gaggcgccgg agctcggggc gagcggcacg atctgcgcgt cggccgaggg agcggtgctc
     3121 ctcgtggacc agggcagcga gcgcctcgag gccacggagt actccaccga cgtcggcgac
     3181 gacgccttcg tgcggcccga cgcacccgac ggcggcaccg gcggatagcg gagcacatca
     3241 cccggtggcg gtcgatacca tcgaccacgt ggacgacccc gaac
//
LOCUS       DATISU010000305         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_67178, whole genome shotgun sequence.
ACCESSION   DATISU010000305 DATISU010000000
VERSION     DATISU010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67178"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VLR27_12155"
     CDS             complement(<1..506)
                     /locus_tag="VLR27_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HSP04252.1"
                     /translation="MEPNEPTNGGAALIRALELEGVEVIFGLPGGAILPVYDPIIDSD
                     IRHVLVRHELGAGHMAEGYAHVTGKPGVCMVTSGPAATNVVTPLADAYLDSVPMVCIT
                     GQVPYAAIGTDAFQECDTTGITMSITKHNYLVSDARDIPRIVREAFHIATTGRPGPVL
                     IDVPKDIV"
     gene            882..1235
                     /locus_tag="VLR27_12160"
     CDS             882..1235
                     /locus_tag="VLR27_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04253.1"
                     /translation="MQIHRIIAKLAALVGLALALTVASAVGAGAQEDPGYEPGYEPGY
                     EAPEDCPPGYEGYPGYEGCGEGEDPGTGGETAAPPAGGTGGMSSLPDTGGVAMVGFAG
                     AAAVAGLALRRFTRS"
     gene            1310..>2417
                     /locus_tag="VLR27_12165"
     CDS             1310..>2417
                     /locus_tag="VLR27_12165"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HSP04254.1"
                     /translation="MWASLDTAQGDLRAISLDSIEEAGGIRPAVDPALDAVGRADTWI
                     SSPFLSPLRAVPVLGSQVEGAQAMVDRLAVVAGAGDAALDQVEAGLARTGEPGGRVEL
                     LTTAIGALDHVEATVAALPEPDTDGLLGPLDSVQQRLEAELDELPERFDELRGHLVTA
                     HDVLAGPTRVLLLAGNNAEMRAGTGMHLSAGVITIEDGDFEASPFFPTGRITTRTEGR
                     AEVPDDVAALHGGIWDVGKEWRTTSTTPDFPTAGASFDQLAELAPIGPVDMVVSLDVP
                     ALAMMLDATGPVEVDGEVVSSANAVDLLLRDNYLRMGEESEANERRDLQSRIAGTIFE
                     TVTERDLDIVDLAASLTRAAEGRNLMGWSEDPGVQ"
BASE COUNT          326 a          883 c          882 g          326 t
ORIGIN      
        1 tcgacgatgt ccttggggac gtcgatgagc accggaccgg gccggccggt ggtggcgatg
       61 tggaacgcct cgcgcacgat gcgggggatg tcgcgggcat cgctgaccag gtagttgtgc
      121 ttggtgatcg acatcgtgat gccggtggtg tcgcactcct ggaacgcgtc ggtgccgatg
      181 gcggcgtagg gcacctgacc ggtgatgcag accatgggca ccgagtcgag gtaggcgtcg
      241 gccagcgggg tcacgacgtt ggtggcggcc gggccggagg tgaccatgca cacgccgggc
      301 ttgccggtga cgtgggcgta gccctcggcc atgtgaccgg cgcccagctc gtggcgcacc
      361 agcacgtggc ggatgtcgga gtcgatgatc gggtcgtaga cgggcaggat cgccccgccc
      421 ggcagcccga agatgacctc cacgccctcg agctcgaggg cccggatgag ggctgctccg
      481 ccgttggtcg gttcgttcgg ttccactgcg gtggtgctcc tgtgtcttcg gcgtcgaggt
      541 ctgcggcggg gcggtcaggt caaggttgcc cggacagccg cagcccgggc gaaaacgacg
      601 acgacccccc ggaacgggag gtcgaggagc gcacgcgcga cgggatgggt cggacgggcg
      661 ctaggcgatg cgtactacga ggtgtcggcg agccatcccg tccatcctgc caccgtgcgg
      721 gcggacccgc caaccccccg ttccgtggcg cgtcgacagc agggaggaat gtccccggag
      781 cgcgtcgaac taccggcctg agctgtcccg atcgcgccgt gtgccgaccg ggctccatgc
      841 tcccgtcgag aggcgggcgg cacacccctg aggagtcaca cgtgcagatc catcgcatca
      901 tcgccaagct cgccgccctg gtcggactgg ccctggcgct caccgtggcc tccgccgtcg
      961 gcgccggcgc gcaggaggat cccgggtacg aaccgggcta cgagcccggc tacgaggcac
     1021 ccgaggactg cccgcccggc tacgagggct acccgggcta cgagggctgc ggcgagggtg
     1081 aggaccccgg caccggcggc gagaccgcgg cacccccggc cggcggcacg ggcggcatga
     1141 gcagcctgcc ggacaccggt ggcgtggcga tggtcggctt cgccggcgcc gcagcggtcg
     1201 cgggcctcgc gctccgccgc ttcacccgaa gctgaggcga gcagccctcc gcagcctggc
     1261 cgtcctcgcc gggctgtacc tgacgctcct cgtcgtccac gccgtcgtgg tgtgggcgtc
     1321 gctcgacacc gcccagggcg acctgcgcgc catctcgctc gactcgatcg aggaggccgg
     1381 tggcatccgc ccggccgtgg acccggccct cgacgcggtg gggcgggccg acacctggat
     1441 ctccagcccc ttcctctccc ccctgcgcgc cgtgcccgtg ctcggatcgc aggtggaggg
     1501 cgcccaggcc atggtcgacc ggctcgccgt cgtcgccggc gccggggacg ccgccctcga
     1561 ccaggtggag gccggcctgg cccgcaccgg tgagcccggc ggccgcgtcg agctgctcac
     1621 caccgccatc ggcgcgctcg accacgtcga ggcgaccgtg gccgccctcc ccgaacccga
     1681 caccgacggg ctcctcggcc cgctcgactc cgtgcagcag cggctggagg ccgagctgga
     1741 cgagctgccg gagcgcttcg acgagctccg cggccacctc gtgaccgctc acgacgtgct
     1801 cgccggcccg acgcgggtcc tgctcctcgc cggcaacaac gccgagatgc gggccggcac
     1861 gggcatgcac ctctccgccg gggtgatcac gatcgaggac ggggacttcg aggccagccc
     1921 cttcttcccc accggccgga tcacgacccg gaccgagggt cgggccgagg tccccgacga
     1981 cgtcgccgcc ctgcacgggg gcatctggga cgtcggcaag gagtggcgca ccacgtccac
     2041 cacgcccgac ttccccaccg ccggggcgag cttcgaccag ctcgccgagc tggctccgat
     2101 cggcccggtc gacatggtcg tgtccctcga cgtccccgcc ctggcgatga tgctcgacgc
     2161 caccgggccg gtggaggtcg acggcgaggt cgtgtcgagc gccaacgccg tcgacctgct
     2221 gctgcgcgac aactacctgc gcatgggtga ggagtccgag gcgaacgagc ggcgcgacct
     2281 gcagagccgc atcgccggca ccatcttcga gaccgtcacc gagcgggacc tcgacatcgt
     2341 cgacctcgcg gcttcgctca cccgggccgc cgagggtcgc aacctgatgg gctggtcgga
     2401 ggaccccggc gtgcagg
//
LOCUS       DATISU010000306         8590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_253231, whole genome shotgun sequence.
ACCESSION   DATISU010000306 DATISU010000000
VERSION     DATISU010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8590
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253231"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VLR27_12170"
     CDS             complement(<1..444)
                     /locus_tag="VLR27_12170"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HSP04255.1"
                     /translation="MSLYELHERPTLDSPVLLVALDGWIDAGGAAARAVESIVAQHDV
                     THVATFDADALLDFRARRPILHLVDGVNTGLTWSTTELHATRSPNGTDVLLLTGVEPD
                     HAWNAFADAVVALTVELGCGMQVGLGAYPAAAPHTRPVQLSATAST"
     gene            complement(450..1469)
                     /locus_tag="VLR27_12175"
     CDS             complement(450..1469)
                     /locus_tag="VLR27_12175"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HSP04256.1"
                     /translation="MQGIEIEAVTAWYQEHVPEVTPPLVFDLIAGGHSNLTYRVTDAD
                     GRRTVLRRPPLGQVLASAHDMGREHRIIAALHPTDIPVAPPLGYVPDDSVNGAPFYVM
                     DFVDGHILRTQDEAMALSPGARANCGDSLVDVLARIHAVDVDAVGLGDLGRKDGFVER
                     QLKRWYGQWEKSKTRELPAIDEVHRVLSADVPEQGPAAIVHGDYRLDNCLVDDQGDVI
                     AVLDWELCTLGDALADVGLLMVYWSEPGDRSPTLGAPTAAEGMRSRQDLLDRYAAASG
                     RDLANIDYYVAFGYWKLACIVEGVYARYIGGSMGTRDTSDFDGFKLQVEALAAQAKAA
                     VDRLG"
     gene            1598..2317
                     /gene="trxA"
                     /locus_tag="VLR27_12180"
     CDS             1598..2317
                     /gene="trxA"
                     /locus_tag="VLR27_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HSP04257.1"
                     /translation="MAYVDVTDQTVQTEVIDRSTQVPVVVDLWAEWCGPCKTLGPIIE
                     KVVDGTGGKVVLAKVDVDANRAIAQAFQVQSIPAVYAMRDGKVVDGFVGALPEHQVQA
                     WVDQLIPSAEESEVSQLIAAGDEASLRQALELEPGNEQAITGLAALLIDGGTNEGKAE
                     ALELLARIPETEATRHLAAQARMANPDVPADVESVLDGLLPKVKSDEDAKQEYLDMLE
                     LMGPTDPRTADYRKKLGAALF"
     gene            2434..2679
                     /locus_tag="VLR27_12185"
     CDS             2434..2679
                     /locus_tag="VLR27_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04258.1"
                     /translation="MFVRRGVTMSPRLRRHEYEHVLQFHRLGRVRFLIRYVAEYLRWR
                     LRRHGHDGAYRRISLEIQAEWRARRELGIGVVDQPST"
     gene            complement(2664..3416)
                     /locus_tag="VLR27_12190"
     CDS             complement(2664..3416)
                     /locus_tag="VLR27_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04259.1"
                     /translation="MMDENEITQRLRDAGDQPVPGEVRASHLSQMHAATPGAAPQPKR
                     FGRLAVAAAAFVGFAVGSTGFAMAGALPDPAQQVAHDVLAVVQVDVENPRNRGQCISN
                     AARTIEDEAAKQAAKQECKDTIKPGKPDHAGQGNGKAKGRPDGAGRPDGHPNDGDPCK
                     GPPPWAGNKMTPAEKDAARAERATTCGRDLTVEEDEPEELEVETEEFEVEPEEPATEA
                     PLTETETEELGTETETEELETEATEPTDQVEG"
     gene            complement(3514..4005)
                     /locus_tag="VLR27_12195"
     CDS             complement(3514..4005)
                     /locus_tag="VLR27_12195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP04260.1"
                     /translation="MRRRRGADPRSIEGVYDALAPAVLGYFRSHRMADPEGLTGDVFV
                     AVTQRLATFEGDDAALRRWVFTVAHHRRVDEIRRATRQAPPGPPSPDAVEPEDPFDPD
                     LVAALATLTDEQRQVVVLRHVADLSVEDVAAITGRSTGSVKMLSARGLEALATRLSPT
                     TVT"
     gene            4128..4793
                     /locus_tag="VLR27_12200"
     CDS             4128..4793
                     /locus_tag="VLR27_12200"
                     /inference="COORDINATES: protein motif:HMM:NF022007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HSP04261.1"
                     /translation="MFGDEDDASGPQVPARLRARWEELLDTTGVGARSLVAGVAVVGL
                     VLGALWWLTRQAPPPVEATLPLVQATAPDTTTTTAGPVVVHVAGAVVAPGVHQLPPGS
                     RVIDAVDASGGLAPDADAGAVNLAAEVVDGSQIYVPRIGEVPPPAPAGPDGGDAAAGP
                     VDLNTADTALLETLPGIGPATAAAIVDHRERHGAFTSVDGLLDVRGIGEAKLAALRDL
                     VRV"
     gene            4786..6651
                     /locus_tag="VLR27_12205"
     CDS             4786..6651
                     /locus_tag="VLR27_12205"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HSP04262.1"
                     /translation="MSEPVALAVSDAVGADRRADLPAVALAVAAWAGAAVARPIPGAV
                     LIGAVGIGAGIHAVARRSGAPGRGGVVVLVVGLALLTSTLGARSLAGLAPVTAGEVDA
                     IVTLVTDPEPTATGRLRVEVRLDGRRLLGEARAPAAIDALRERLAGDRAVVRGVVEPF
                     EHRQDWTVARHLAGRLRIESVTGVGGGSLHAGAANGLRRVLDRGAMSLPDRHRSLLAG
                     LTLGDDRDQPAELTADFRASGLTHLLAVSGQNVVFVLAVAGPLLRRLRIWPRFVLALA
                     VVAGFAFLTRFEPSVVRAAFVAGVALWASTSGRPSGGLRHLALAVCALLVVDPLLTRS
                     VGFRLSVAACLGVLLLSAPIVARLPGPLWAREALGMTAGAQLAVAPVLVPTFGAMPLA
                     ALPANVAAAPLAGALMVWGVTAGAVAGVAGGVVGELLHLPSALGLWALEMVASLSAAL
                     PLGHVDLRHVGVVALGGVLFATRRPWARGAAVVLLAAAVLTPATVRTPPGASAAGWAA
                     TVWSDGPVAVVDLRAGASGVEVLDVLRRRQVHVVGMILVRAGRPELADVVGAVRARFP
                     VHLVLAPEGSPVPHHVVPEPGLITAVAGFDVQISSVTPVLDARIGRGPEVPPVQR"
     gene            6661..7236
                     /locus_tag="VLR27_12210"
     CDS             6661..7236
                     /locus_tag="VLR27_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04263.1"
                     /translation="MLLALGPLQLDTTMRTVVIGVADSPDDACIDRATSGADAVWLRG
                     AATVAQVRAAAERCARPLGVTVDDLDRSAELEAAGAVAVEVRSPDADADLAGLGTRPG
                     LSLWCAPGLARRALDAGVAPERLIVEGSPWDGPGVVGTTVVGDGPATWGSVVHALLDG
                     AGAIRTTDARAVRRVVTVTDRLLSARDGGRT"
     gene            7233..8213
                     /gene="holA"
                     /locus_tag="VLR27_12215"
     CDS             7233..8213
                     /gene="holA"
                     /locus_tag="VLR27_12215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HSP04264.1"
                     /translation="MSRPPVILIKGADDILRSARRSAVVAEQVGDADATLVVDEPTLD
                     DDERLRPLVDAAQTSPFFTDHRVVVGRSIERFKAKDDLAGLLDYLADPLPTTVLVLDW
                     GGTGRVPKALSDAITAAGGETISTDPGRDVGSFVDDAVSKARLQLERDAQRHLVDWLG
                     DDPGKLPGVLAVLASTYGEGARLSVADVDPFLGDAGGVPPWDLTDAIDRGDIAAAITT
                     LTRMLRSGRHPMQVMATLHTHFQRIMRLDGAGVRGEKEAATVLGMKGSTFPAKKALAQ
                     SRRLGSDGVRESIRLLARTDLELRGTVDWPVELSMEVLVARLARVAKASR"
     gene            complement(8280..>8590)
                     /gene="rpsT"
                     /locus_tag="VLR27_12220"
     CDS             complement(8280..>8590)
                     /gene="rpsT"
                     /locus_tag="VLR27_12220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HSP04265.1"
                     /translation="AHATFATPEVPHVANIKSQIKRNRQNEKARQRNKTVRSELQTRT
                     KKVLTAAQNGEETAESLKAAIRRIDTAATKGVLHKNTAARKKSRLIKQVRRLESAEQA
                     "
BASE COUNT          987 a         3082 c         3241 g         1280 t
ORIGIN      
        1 ggtggaggcg gtggccgaga gctgcacggg ccgggtgtgc ggcgcagccg ccgggtaggc
       61 gccgaggccg acctgcatgc cacagcccag ttcgacggtc agggccacga cggcgtcggc
      121 gaacgcgttc catgcgtggt cgggctccac cccggtgagg agcaggacat cggtgccgtt
      181 cggggaccgg gtggcgtgga gctcggtggt ggaccaggtg agccccgtgt tgacgccgtc
      241 gacgaggtgc aggatcggac gccgggcccg gaagtcgagc agcgcgtcgg cgtcgaacgt
      301 cgccacgtgg gtcacgtcgt gctgcgccac gatggactcg acggcgcgtg cggcggcacc
      361 gcccgcgtcg atccatccgt cgagcgccac gagcaggacc ggtgagtcca gggtcggacg
      421 ctcgtgcagc tcgtagaggg acaccggcgt cagcccagcc gatccaccgc ggccttcgcc
      481 tgcgcggcga gggcctcgac ctggagcttg aagccgtcga agtcggacgt gtcccgcgtg
      541 cccatcgagc cgccgatgta gcgcgcgtag acgccctcga cgatgcaggc cagcttccag
      601 tagccgaagg cgacgtagta gtcgatgttg gcgaggtccc gtcccgatgc ggcggcatag
      661 cggtcgagga ggtcctggcg ggaccgcatc ccctcggccg cggtcggggc cccgagggtg
      721 ggggagcgat cgcccggctc ggaccagtac accatcagca ggccgacgtc ggcgagggcg
      781 tcaccgaggg tgcacagctc ccagtcgagc accgcgatga cgtcgccctg gtcgtcgacc
      841 aggcagttgt cgagtcggta gtcgccgtgg acgatggccg ccggcccctg ctcgggcacg
      901 tcggcgctga ggacccggtg cacctcgtcg atcgcgggca gctcacgggt cttggacttc
      961 tcccactgcc cgtaccagcg cttgagctgg cgctccacga agccgtcctt gcgaccgagg
     1021 tcgccgaggc cgaccgcgtc gacgtcgacc gcgtggatgc gggcgagcac gtcgacgagc
     1081 gagtcgccgc agttggcacg cgccccgggc gacagcgcca tggcctcgtc ctgcgtgcgc
     1141 aggatgtgcc cgtcgacgaa gtccatgacg tagaagggcg caccgttcac ggagtcgtcg
     1201 gggacgtagc cgaggggcgg ggcgacgggg atgtcggtgg ggtgcagcgc ggcgatgatc
     1261 cggtgctcgc gacccatgtc gtgggcgctg gccaggacct ggccgagggg cgggcggcgc
     1321 agcacggtgc ggcggccgtc ggcatcggtg acccggtagg tgaggttcga gtggccgccg
     1381 gcgatcaggt cgaacacgag cggcggcgtc acctcgggta cgtgctcctg gtaccaggcc
     1441 gtgaccgcct cgatctcgat tccctgcacg tccgccactg gacctccctc tcggtgtcgg
     1501 tgtgtgggga ggtcaaccta gtgagcgccc ctcgccgacg ttgatccacc cggagtcgga
     1561 ggcggcgaga tcggacctcg ccggtaggtt ggccgccatg gcatacgtcg acgtgaccga
     1621 ccagacggtc cagaccgagg tgatcgaccg ctccacccag gtgccggtcg tcgtcgacct
     1681 ctgggccgag tggtgcgggc cgtgcaagac cctcggtccg atcatcgaga aggtggtcga
     1741 cggcaccggc ggcaaggtcg tgctcgccaa ggtcgacgtc gacgccaacc gcgccatcgc
     1801 ccaggccttc caggtccagt cgatccccgc cgtgtacgcc atgcgcgacg gcaaggtcgt
     1861 cgacggcttc gtcggcgcac tgcccgagca ccaggtccag gcctgggtgg accagctgat
     1921 cccctcggcc gaggagtccg aggtctccca gctgatcgcc gccggcgacg aggcgtcgct
     1981 gcgccaggcg ctcgagctcg agcccggcaa cgagcaggcc atcaccgggc tggctgcgct
     2041 gctcatcgac ggcggcacca acgagggcaa ggccgaggcg ctcgagctgc tggcccgcat
     2101 ccccgagacc gaggccaccc ggcacctcgc cgcccaggcc cgcatggcca accccgacgt
     2161 ccccgccgac gtggagtcgg tcctcgacgg gctgctgccg aaggtcaagt ccgacgagga
     2221 cgccaagcag gagtacctcg acatgctcga gctgatgggc cccaccgacc cccgcaccgc
     2281 cgactaccgc aagaagctgg gcgccgcgct cttctaggcc cgagcccggc tggtctcccc
     2341 gtgcgacgcc ggccacctcc caggatcgac gacctccgca tcgtccacgg gttcgctgcg
     2401 cccggcgcca gcgccacgac cctcggacgg accgtgttcg tgcgccgagg cgtcacgatg
     2461 agcccccggc tgcgtcgcca cgagtacgag cacgtcctgc agttccaccg cctcggccgc
     2521 gtccggttcc tcatccgcta cgtcgccgag tacctgcgct ggcgcctgcg ccgccacggc
     2581 cacgacggcg cctaccggcg catctccctg gagatccagg ccgagtggcg ggcccgccgc
     2641 gagctcggca tcggcgtggt ggatcagccc tcgacctgat ccgtgggttc ggtcgcctct
     2701 gtctcgagct cctcggtctc ggtctcggtg ccgagctcct cggtctccgt ctcggtcaag
     2761 ggcgcctcgg tggccggctc ctcgggctcg acctcgaact cctcggtctc gacctcgagc
     2821 tcctcgggct cgtcctcttc gaccgtgagg tcccgtccgc aggtggtggc gcgctcggcc
     2881 ctcgccgcgt ccttctccgc aggggtcatc ttgttgccgg cccacggcgg cgggcccttg
     2941 cacggatcgc cgtcgttcgg gtggccgtcg ggccgaccgg caccgtcggg ccggcccttg
     3001 gccttgccgt tgccctgacc ggcgtgatcg ggcttgcccg gcttgatcgt gtccttgcac
     3061 tcctgcttgg cggcttgctt cgcggcctcg tcctcgatgg tgcgggcggc gttcgagatg
     3121 cactggccgc ggttcctcgg gttctcgacg tccacctgga cgacggcgag cacgtcgtgg
     3181 gcgacctgct gggccgggtc ggggagggcg ccggccatgg cgaagccggt ggagccgacc
     3241 gcgaagccca cgaaggcggc ggcggccacg gcgaggcggc cgaagcgctt gggctggggt
     3301 gctgcccccg gagtggcggc gtgcatctgg ctgaggtggc tcgcacggac ctcgccgggg
     3361 accggctggt cgccggcgtc acggaggcgc tgggtgatct cgttctcgtc catcaccccc
     3421 gtgatcgtcc ggcgggccga tccggtcacg gtgctgtcgg agtttctcgg tgcagggcgt
     3481 tgcgccagcg caacgcgaga gcccgagaac cggtcaggtc acggtcgtgg gggagaggcg
     3541 ggtcgccagc gcctccaggc cgcgagcgga cagcatcttc accgacccgg tgctgcgccc
     3601 ggtgatggcg gcgacgtcct ccacggagag gtcggcgacg tggcgcagca ccacgacctg
     3661 gcgctgctcg tcggtgaggg tggcgagggc ggcgaccagg tcggggtcga aggggtcctc
     3721 gggttcgacc gcgtccggcg agggcggtcc gggcggggcc tggcgggtgg ctcgccggat
     3781 ctcgtccacc cggcggtggt gggcgacggt gaacacccag cggcgcaggg cggcgtcgtc
     3841 gccctcgaag gtggcgaggc gctgggtgac ggcgacgaag acgtcgccgg tgaggccctc
     3901 ggggtcggcc atgcggtggg agcggaagta gcccagcacc gccggcgcga gggcgtcgta
     3961 gaccccttcg atcgatcggg ggtccgcacc ccggcggcgt cgcacgggcc catcatccca
     4021 ccgggccccg gaccgagcgc ggctggagcg gccatcgcct accctggcgc tcgacttccc
     4081 cggtcgaacc gtcgtgcctg cgggccgacg tgtgcgcgga ggaagccatg ttcggcgacg
     4141 aggatgacgc ctcgggtccc caggtgccgg cccgcctgcg ggcgcgctgg gaggagctgc
     4201 tcgacacgac gggcgtcggt gcccggtcgc tcgtggccgg cgtcgccgtg gtcgggctcg
     4261 tgctcggggc gctgtggtgg ctgacccggc aggcgccccc accggtcgag gcgaccctcc
     4321 cactggtgca ggccaccgca cccgacacga ccacaacgac ggccggcccg gtcgtggtcc
     4381 acgtcgccgg tgccgtcgtc gcacccggcg tgcaccagct gccgccgggt tcccgggtca
     4441 tcgacgcggt cgacgcctcc ggcgggctcg cgccggatgc cgatgcgggg gcggtcaacc
     4501 tggcggccga ggtcgttgac gggtcccaga tctacgtgcc ccgcatcggt gaggtgccgc
     4561 cgccggcgcc cgccgggccg gacggcggtg acgcggccgc aggtccggtg gatctgaaca
     4621 cggcggacac ggcgctgctc gagacgctgc ccggcatcgg cccggccacc gcggcagcga
     4681 tcgtggacca ccgggagcgg cacggtgcct tcacctccgt cgacgggctc ctcgacgtgc
     4741 gaggcatcgg cgaggcgaag ctggctgcgc tgcgggacct ggtgcgtgtc tgagcccgtc
     4801 gccctcgcgg tcagcgacgc cgtcggcgcc gaccggcggg ccgatctccc cgccgtggcg
     4861 ctggcggtcg ccgcatgggc gggcgccgca gtggcacgtc cgatccccgg ggcggtgctg
     4921 atcggcgcgg tcgggatcgg cgccggcatc catgccgtgg cccgccgttc cggtgccccc
     4981 ggtcgaggtg gtgtggtggt gctcgtagtg gggctggcgt tgctcacctc gacgctcggt
     5041 gcccggtcgc tggccggcct ggccccggtc accgccggcg aggtcgatgc gatcgtcacg
     5101 ctcgtcaccg atcccgagcc caccgcgacc ggccgtctgc gggtcgaggt ccgcctcgac
     5161 ggtcgacggc tcctcggcga ggcacgagcg ccggcggcga tcgatgcgct gcgggagcgc
     5221 ctggcagggg accgggctgt ggttcggggc gtggtggagc ccttcgagca ccgccaggac
     5281 tggaccgtgg cccgccacct cgccggccgc ctccgcatcg aatcggtcac cggggtcggc
     5341 ggcgggtcgc tccatgccgg tgccgccaac ggtctgcgac gggtgctcga ccggggggcg
     5401 atgagcctgc ccgaccgcca ccgctccctt ctcgccggcc tcacgctcgg cgacgaccgg
     5461 gaccagccag cggagctcac cgccgacttc cgggcatcgg gtctcaccca cctcttggcc
     5521 gtctcgggcc agaacgtcgt cttcgtcctg gcggtcgccg gtccgctgct gcgtcggctc
     5581 cggatctggc cgcgcttcgt cctcgccctg gcggtggtgg ccggcttcgc cttcctcacc
     5641 cgcttcgagc cgtcggtggt gcgggcggcg ttcgtggccg gcgtcgcgct gtgggcatcg
     5701 acctcggggc ggccatcggg cgggctccgc cacctcgcgc tggcggtctg cgccctgctg
     5761 gtggtggatc cgctgctcac ccgatcggtg gggttccgcc tgagcgtcgc cgcctgcctc
     5821 ggcgtgctgc tgctctccgc gccgatcgtg gctcgactgc ccggccccct ctgggcgcgg
     5881 gaggccctgg gcatgaccgc cggcgcccag ctcgcggtgg cgccggtcct ggtcccgacg
     5941 ttcggcgcca tgccgctcgc cgccctgcct gcgaacgtgg ccgcggcccc gctggcgggc
     6001 gcgctgatgg tgtggggcgt gaccgcgggt gcggtcgccg gcgtggcggg cggggtggtg
     6061 ggcgagctgc tccacctccc gtcggccctg ggcctgtggg cgctcgagat ggtggcgtcc
     6121 ctgagcgccg cgctgcccct cggccacgtc gacctgcgcc acgtgggggt cgtggccctc
     6181 ggtggcgtgc tcttcgccac ccgtcgcccc tgggcgcgag gtgccgcggt ggtgctgctc
     6241 gccgccgcag tgctcacccc ggccacggtg cggacacccc ccggggcgtc ggctgccggc
     6301 tgggcagcga ccgtgtggag cgacggcccg gtggcggtgg tcgatctgcg cgccggggcg
     6361 tcgggagtgg aggtgctcga tgtgctccgc cggcggcagg tgcacgtcgt cgggatgatc
     6421 ctggtccgcg ccggccgtcc ggagctggcc gatgtcgtcg gtgcggtccg ggcccggttc
     6481 cccgtccacc tcgtgctcgc acccgagggg tcgccggtgc cccaccacgt ggtgcccgag
     6541 ccggggctca tcacggccgt ggccggcttc gacgtccaga tctcctcggt gacgccggtg
     6601 ctggacgccc gcatcggacg aggaccggag gtgccgccgg tgcagcgata ggttcgacag
     6661 gtgctcctgg cgctgggtcc cctgcagctc gacacgacca tgcgcacggt cgtgatcggt
     6721 gtcgcggact caccggacga tgcctgcatc gacagggcga cgtcgggcgc cgacgccgtg
     6781 tggctccgcg gtgcagccac cgtggcgcag gtgcgcgccg ctgccgagcg ctgcgctcgg
     6841 cccctcgggg tcaccgtgga cgacctcgac cggtcggccg agctggaggc cgccggggcg
     6901 gtggcggtcg aggtgcgatc ccccgatgcc gatgctgacc tcgccgggct gggaacccgc
     6961 cccgggctga gcctgtggtg cgcgcccggc ctcgcccgtc gggcgctcga tgccggcgtg
     7021 gcgcccgagc gcctgatcgt cgagggcagc ccctgggacg gtcccggcgt ggtcggtacc
     7081 actgtcgtgg gcgacgggcc cgccacgtgg ggttcggtgg tgcacgccct gctggacggt
     7141 gccggtgcga tccggaccac cgacgctcgg gccgtgcgcc gggtcgtgac cgtgacggat
     7201 cggctcctgt cggcgcggga cggaggacgg acgtgagccg gcctccggtc atcctgatca
     7261 agggcgccga cgacatcctc cgctccgccc gccggtctgc tgtggtcgcc gagcaggtgg
     7321 gcgacgccga cgccaccctc gtcgtcgacg agcccacgct ggacgacgac gagcgcctcc
     7381 gcccgctcgt cgacgcggca cagaccagcc cgttcttcac cgaccaccgc gtggtggtcg
     7441 ggcgcagcat cgagcggttc aaggccaagg acgacctcgc cggcctgctc gactacctgg
     7501 ccgaccccct gccgaccacg gtgctggtgc tggactgggg tggcacggga cgggtcccca
     7561 aggccctctc ggatgccatc acggcggccg gtggggagac gatcagcacc gatcccggtc
     7621 gcgacgtcgg cagcttcgtc gacgacgccg tgtcgaaggc ccgactgcag ctcgagcggg
     7681 acgcccagcg gcatctggtc gactggctgg gcgacgaccc gggcaagctc cccggggtgc
     7741 tcgcggtcct ggcgtccacg tacggggagg gcgcccggct cagcgtcgcc gacgtcgacc
     7801 cgttcctggg cgacgccggc ggggtcccgc cgtgggacct caccgatgcc atcgatcgcg
     7861 gcgacatcgc ggccgccatc acgaccctga cccgcatgct ccgttccggc cgccacccga
     7921 tgcaggtgat ggccaccctg cacacccact tccagcgcat catgcgcctc gacggcgccg
     7981 gcgtgcgggg ggagaaggag gcggcgaccg tcctcggcat gaagggctcg acgttcccgg
     8041 ccaagaaggc cctggcccag tcgcgccggc tcgggtccga cggcgtgcgg gagtccatcc
     8101 ggctgctcgc ccgcaccgac ctcgagctgc gcggcacggt ggactggccg gtcgagctca
     8161 gcatggaggt cctcgtcgcc cgcctggccc gggtcgcgaa ggcgtcgcgc taggtccgag
     8221 cccggacgcg gaacgacccc gccgcagcgg ggtcgtccag aacgcgagga gcgtcggggt
     8281 caggcctgct cggccgactc caggcgacgg acctgcttga tcaggcggga cttcttgcga
     8341 gccgccgtgt tcttgtggag cacgcccttg gtggccgcgg tgtcgatgcg acggatggcg
     8401 gccttgagcg actcagcggt ctcctcgccg ttctgggcgg cggtgagcac cttcttggtg
     8461 cgggtctgca gctcgctgcg caccgtcttg ttgcgctggc gcgccttctc gttctggcgg
     8521 ttgcgcttga tctggctctt gatgttggcc acgtggggga cctctggggt tgcgaaggtg
     8581 gcgtgcgcgc
//
LOCUS       DATISU010000307         7351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_719026, whole genome shotgun sequence.
ACCESSION   DATISU010000307 DATISU010000000
VERSION     DATISU010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719026"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VLR27_12225"
     CDS             complement(<1..626)
                     /locus_tag="VLR27_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSP04266.1"
                     /translation="MPFRTIPSLTGRRVLITGAARGIGAALAERLHDRGARVALAGLE
                     PELLADVAGRLDAPWWSCDVTDRDQVESTVQGAVDALGGLDVVVANAGIAAQLPLVGG
                     DPAIFERIMDVNVLGAYNTLRAAGPHISHDQGYAVAVSSLGAAVHMPLMGAYSASKAA
                     VEALGNTLRAEIAPTGARVGVAYFAELDTEMTSRGFGTEAAKRFLGGRT"
     gene            675..2417
                     /locus_tag="VLR27_12230"
     CDS             675..2417
                     /locus_tag="VLR27_12230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HSP04267.1"
                     /translation="MYRKLGILGAALVACAAFIAPSAVADDVDPQPFVTEDHRVPMSD
                     GVDLLARVGGRGPLVDGRLPARPVIIELSPYAPGCCPEYGGDGFNNVQVHIRGTGDSG
                     GAFDALGPRTQRDVAEILAWACEQPWSNGRLGLYGFSASAITAYNSLHQELPCLEGAV
                     LGAGTHELYRDLLYPGGIPNGVPAAGVFGLIGGPAMATVPERLQRDPGSLLPLTTAYG
                     QLAVDYLAHPTLDEWWRARGMRGDVNDVPVLVVNGFFDVESRGAFEAFQELADDGAHL
                     YVVGAHDGVPAGSGGAGDVTRRWLEHHVAGTPNGVDDQPTVQLWMADGDRVDMLDGDF
                     VRVDADAWPVPGTEWVPLHLGAARSGTATSSNDGVLSLSPDPEPTAIPHVPIPSLATA
                     TDAPTTSLLGVFNGSPELTTMNGPEALGLSWTTEAIAEDVLLAGPASLELALTSAVPE
                     SDLWVVISDVHPDGTAHPMATGRLRTTYPDLVPERTRTDATGAVIQPYADHSSKRSVT
                     PGEEHRFHVELWPIGNRFQEGHRLRVHVVGSSLFSVPTATGPQLVRVGGDDGGSVLRV
                     PVLPGSDLRSALGG"
     gene            complement(2419..3978)
                     /locus_tag="VLR27_12235"
     CDS             complement(2419..3978)
                     /locus_tag="VLR27_12235"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HSP04268.1"
                     /translation="MKELVYHRLLLPAVERYGDREAIIDGDHRGTLAEHLDRTTRLAS
                     ALRTELGVGPGDRFAIMATNSHQYLELYHAAFLGAGVVNPLNLRLAVAELEFILRDSG
                     TKVVFVDFLFANNIDMIREAAGIEKVVLIGPSMDSPHDIAYEDLIAAGEPTIPEEPEE
                     DDPVVLMYTGGTTGLPKGVLLDQRAEMLNLYHVIMAVGIDEGSTYLHQTPMFHAASMG
                     GLLGIPASGARSVFLPLFDPEKAMDTIEEHGVDWTVMVPTMVGMVLRHPAFRPERLAS
                     LRKLTYGASPMPEAILERLLELYPELELSQGYGMTEASAVLTFLNAEDHRRGGDVLRS
                     AGRPVPGVVLSVQDEDGNVLPTGETGEICARAGNFMREYWNRPEETAEAFRGGWYHTG
                     DAGHIDDEGFVYLVDRVKDMIVTGGENVYSSEVEDAIGSHPEVDQVAVIGIPSEKWGE
                     AVHAIVVRVEGSSVEAEELIAHARKHIAGYKVPKSIDFRDEPLPLSGAMKVLKRELRA
                     PYWEGHDRGVG"
     gene            complement(4031..6043)
                     /locus_tag="VLR27_12240"
     CDS             complement(4031..6043)
                     /locus_tag="VLR27_12240"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSP04269.1"
                     /translation="MDQARSAAGAERLAEVDGVVGTLLDLVEVDAGWESAFEAAAADA
                     LSAVVVDGAGAARQALAALTAGDATGAVLALGVDRTARTAPPVGEPVRSHVRSNRPGV
                     DALLDAVVGAAVRIDGDWAAGLDVALAHPDAVVVTADGARFGAGAWRVGGSATGATGA
                     ALEEARTRVDAAAEAVTAATATRDAARTRFAELKRTAGDARQAAEQTAAALRRAADGL
                     ARVEQQATEAAEELEGQSGVRAEVEQRLRTERDRVAELEAMLPALEAEEAAAAEAARA
                     MTEARAALDDRAAAVATLRSDLEIRVAQVEERRTSLQARLVEVDARVARNQQAAQEAA
                     VRREALDVRARALDGLATACQASLTEVEDQLVGLRAKRQEQSEAQRAVAVALDGLRRE
                     RVQVEKQLNEARERIQRADLELNEINLRIETSVETMRRDLDVDPETAIAAECPELAEN
                     VAPAARARELERELRLMGPINPLALEEYEALQERHEFLQGQLDDVKGSRRELSKVIRA
                     IDEEIVNVFAAAFADVSQNFTQLFSTLFPGGTGSIKLTDPEDLLETGIEVEAKPSGKN
                     VRKLSLLSGGERSLTALAFLFAVFRSRPSPFYVMDEVEAALDDVNLHRFLELVAEFRR
                     EAQLVIVSHQKRTMEAADILYGVTMSPGGSSKVVSERGAAKHAGAA"
     gene            complement(6203..>7351)
                     /locus_tag="VLR27_12245"
     CDS             complement(6203..>7351)
                     /locus_tag="VLR27_12245"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04270.1"
                     /translation="SSVRSQKMDDVIFAGTSKRSALGRAEVALTLDNTAGLLPIDFSE
                     VTITRTLFRTGESEYSINGVPCRLLDIQELLSDSGVGRMQHVIISQGQIDAVLNAKPT
                     ERRLIIEEAAGVLKFRKRKEKAERRLASTEANLTRVQDLLREVRRGLRPLERQAEAAR
                     RHGDLVTELDALRRFLAGRELARLRSRLDTTTHDRAQHAGTEETLRARLRDLDASVLR
                     SEAELNAMGGADVGDELTRYEALRERGRGIAAVLAERQRSLAKERESTVDAALIASLE
                     ADQARLRDELATIESTAAELLPEAEEVDRAERGLADARATFEERWGEGIASPSGEAAE
                     VRGQLGGLRQAVSRSLAELERTDGRTGSLADKRDRLTAEAERLRSEVR"
BASE COUNT          939 a         2625 c         2758 g         1029 t
ORIGIN      
        1 gtgcgaccgc cgaggaaccg cttcgccgcc tcggtgccga agccccggct ggtcatctcg
       61 gtgtcgagct cggcgaagta ggcgaccccg acccgggcgc ccgtgggggc gatctcggcg
      121 cgcagggtgt tgccgagggc ctccaccgcc gccttggatg cgctgtaggc ccccatcagg
      181 ggcatgtgga cggcggcgcc gagggaggag acggccaccg cgtagccctg gtcgtgggag
      241 atgtggggcc cggccgcccg gagggtgttg tacgcaccga gcacgttcac gtccatgatc
      301 cgctcgaaga tcgccgggtc gcccccgacc agggggagct gggcggcgat gccggcgttg
      361 gcgacgacga cgtcgaggcc accgagcgca tcgaccgcgc cctggaccgt ggactccacc
      421 tggtcgcgat cggtgacgtc gcaggaccac cacggcgcgt cgagccgacc ggcgacgtcg
      481 gcgaggagct cgggttcgag cccggcgagg gcaacgcgag cgccacggtc gtgcagtcgc
      541 tcggcgagag cggcgccgat gccgcgggca gcaccggtga tgaggacccg gcgaccggtg
      601 agtgagggga tcgttcggaa gggcacggaa cggacactag cgatcgatga acaatgttgt
      661 ctatcgttca cgagatgtac cggaagctgg ggatcctggg tgccgcgctc gtcgcgtgcg
      721 ccgccttcat cgcaccgtcc gccgtggccg acgacgtcga tccgcaaccg ttcgtcaccg
      781 aggaccaccg ggtcccgatg tcggacgggg tggacctgct ggcccgggtc ggcggacggg
      841 gtcccctcgt cgacggccgg ctgccggccc gaccggtcat catcgagctc agcccgtacg
      901 cgccgggctg ctgtcccgag tacggcggcg acgggttcaa caacgtccag gtccacatcc
      961 ggggcaccgg cgacagcggc ggcgccttcg acgccctcgg cccccgcacc cagcgtgacg
     1021 tggcggagat cctggcgtgg gcgtgcgagc agccgtggag caacgggcgc ctcggcctct
     1081 acggcttctc cgccagcgcg atcaccgcct acaacagcct ccaccaggag ctgccgtgcc
     1141 tcgagggcgc ggttctcggt gcgggcaccc acgagctgta ccgcgacctg ctctaccccg
     1201 gcggcatccc caacggcgtc ccggccgccg gcgtgttcgg cttgatcggc gggccggcga
     1261 tggccacggt gcccgagcgc ctccagcggg accccggttc gctgctcccc ctcaccaccg
     1321 cgtacgggca gctcgcggtc gactacctgg cccacccgac gctcgacgag tggtggcgag
     1381 cgcgcggcat gcggggcgac gtgaacgacg tccccgtgct cgtcgtcaac gggttcttcg
     1441 acgtcgagtc gcgcggcgcc ttcgaggcgt tccaggagct cgccgacgac ggcgcccacc
     1501 tctacgtggt cggcgcccac gacggcgtgc ccgccggatc gggcggcgcc ggtgacgtga
     1561 cccggcggtg gctcgagcac cacgtcgccg gcacccccaa cggcgtcgac gaccagccca
     1621 ccgtgcagct ctggatggcc gacggcgacc gggtcgacat gctggacggc gacttcgtcc
     1681 gggtcgacgc cgacgcctgg ccggtcccgg gcaccgagtg ggtcccgctc cacctcggcg
     1741 cggcgcggag cggcacggcc acctcgagca acgacggtgt gctgtccctc tcccccgacc
     1801 ccgagccgac cgcgatcccg cacgtgccga tcccgtcgct ggcgaccgcc accgacgccc
     1861 ccaccacgtc gctgctcggc gtgttcaacg gttcacccga gctcaccacc atgaatggcc
     1921 cggaggcgct ggggttgtcg tggacgaccg aggcgatcgc ggaggacgtg ctcctggccg
     1981 ggccggcgtc gctcgagctc gccctcacct ctgcggtgcc cgagagcgac ctgtgggtcg
     2041 tgatctccga cgtccacccc gacggcaccg cccaccccat ggccacgggc cgcctccgca
     2101 cgacctaccc cgacctggtg ccggagcgca cccgaaccga cgcaacgggc gccgtgatcc
     2161 agccctacgc cgaccactcg tcgaagcggt ccgtcacgcc gggcgaggag caccggttcc
     2221 acgtcgagct gtggccgatc ggcaaccggt tccaggaagg ccaccggctg cgggtccacg
     2281 tcgtggggtc gtcgctcttc agcgtgccca ccgccaccgg cccgcagctg gtgcgggtcg
     2341 gcggcgacga cggcggttcg gtgctgcggg tgccggtcct gccgggcagc gacctgcggt
     2401 cggcgctcgg cgggtaggtc agccgacgcc gcggtcgtgg ccctcccagt acggggcccg
     2461 cagctcgcgc ttgaggacct tcatcgcccc cgagagcggc aggggctcgt cgcggaagtc
     2521 gatggacttc ggcaccttgt agccggcgat gtgcttgcgg gcgtgggcga tcagctcctc
     2581 cgcctcgacc gaggagccct ccacccgcac gacgatggcg tggaccgcct cgccccactt
     2641 ctcgctcggg atcccgatca ccgccacctg gtcgacctcc gggtggctgc cgatcgcgtc
     2701 ctcgacctcg gaggagtaca cgttctcccc gccggtgacg atcatgtcct tgacccggtc
     2761 gaccaggtac acgaagccct cgtcgtcgat gtggcccgcg tcgccggtgt ggtaccagcc
     2821 gccccggaac gcctcggcgg tctcctcggg acggttccag tactcccgca tgaagttccc
     2881 ggcccgggcg cagatctccc ccgtctcccc cgtcggcagg acgttgccgt cctcgtcctg
     2941 gacgctgagc accacgccgg ggaccggacg ccccgccgac cggaggacgt cgccgccacg
     3001 ccggtggtcc tcggcgttga ggaaggtgag cacggcggac gcctcggtca tgccgtagcc
     3061 ctggctcagc tcgagctccg ggtacagctc cagcagccgc tcgaggatcg cctcgggcat
     3121 cggcgacgcc ccgtaggtca gcttgcggag cgaggcgagg cgctcgggcc ggaacgcggg
     3181 gtggcgcagc accatgccca ccatcgtggg caccatcacg gtccagtcga cgccgtgctc
     3241 ctcgatggtg tccatggcct tctcggggtc gaacagcggc aggaacaccg agcgtgcacc
     3301 cgaggcgggg atgccgagca gtccgcccat cgaggcggcg tggaacatgg gggtctggtg
     3361 caggtaggtc gagccctcgt cgatgcccac cgccatgatc acgtggtaca ggttcagcat
     3421 ctcggcccgc tggtcgagca gcacgccctt gggcagcccc gtggtgccgc ccgtgtacat
     3481 gagcacgacg gggtcgtcct cctcgggctc ctctgggatc gtcggctcgc cggcggcgat
     3541 caggtcctcg taggcgatgt cgtggggcga gtccatgctc gggccgatga gcacgacctt
     3601 ctcgatcccg gccgcctcgc ggatcatgtc gatgttgttg gcgaacagga agtcgacgaa
     3661 gaccaccttg gtgcccgagt cccgcaggat gaactccagc tccgccaccg cgaggcgcag
     3721 gttcagcggg ttgacgacgc cggcacccag gaaggcggcg tggtacagct cgaggtactg
     3781 gtggctgttg gtggccatga tggcgaagcg atcgccgggc ccgacgccca gctcggttcg
     3841 cagcgcggaa gccaggcgcg tcgtgcgatc gaggtgctcg gccagcgtgc cgcggtggtc
     3901 gccgtcgatg atcgcctcgc ggtcgccgta gcgctccact gccggcagca gcagacggtg
     3961 gtacacgagc tccttcacga ccagaccccc ggttcgtcat ccgacagggg acgatgatcg
     4021 cacgtcgtgg ctaggcggcg cccgcgtgct tggctgcgcc ccgctcggac acgaccttgg
     4081 acgagccgcc cggcgacatg gtgacgccgt agaggatgtc ggccgcctcc atggtgcgct
     4141 tctggtgcga gacgatcacg agctgggcct cgcggcggaa ctcggcgacg agctcgagga
     4201 accggtgcag gttcacatcg tcgagcgcgg cctcgacctc gtccatgacg tagaagggcg
     4261 agggccggga gcggaacacg gcgaacagaa acgccagcgc ggtcagcgag cgctcgccac
     4321 ccgacagcag cgacagcttg cggacgttct tgcccgacgg cttggcctcg acctcgatgc
     4381 cggtctcgag caggtcctcg gggtcggtca gcttgatcga accggtgccg cccgggaaga
     4441 gcgtggagaa cagctgcgtg aagttctgcg acacgtcggc gaaggcggcg gcgaagacgt
     4501 tgacgatctc ctcgtcgatg gcccggatga ccttcgagag ctcccgccgg ctgcccttga
     4561 cgtcgtcgag ctggccctgg aggaactcgt gccgctcctg gagggcctcg tactcctcga
     4621 gggcgagtgg gttgatcggg cccatgaggc gcagctcgcg ctcgagctcg cgggcccgtg
     4681 cggccggcgc cacgttctcg gccagctcgg ggcactcggc ggcgatggcc gtctcggggt
     4741 cgacgtcgag gtcgcgacgc atggtctcga ccgaggtctc gatccgcagg ttgatctcgt
     4801 tgagctcgag gtcggcgcgc tggatgcgct cgcgcgcttc gttgagctgc ttctcgacct
     4861 ggacgcgctc gcggcgcagc ccgtcgaggg cgacggcgac ggcccgctgg gcctcggact
     4921 gctcctggcg cttggcccgg aggccgacga gctggtcctc gacctcggtc agcgacgcct
     4981 ggcacgcggt ggccaggccg tcgagggctc gggcccggac gtcgagggcc tcgcgacgga
     5041 cggcagcctc ctgtgcggcc tgctggttgc gggccacccg ggcgtcgacc tcgacgaggc
     5101 gggcctgcag cgacgtgcgc cgctcctcca cctgggcgac gcggatctcg aggtcggagc
     5161 gcaacgtcgc cacggcggcg gcacggtcgt cgagcgcggc gcgggcctcg gtcatggccc
     5221 gggcggcctc ggcagcggcg gcctcctcgg cctcgagggc cgggagcatg gcttcgagct
     5281 cggcgacgcg gtcccgctcg gtgcgcaggc gctgctcgac ctcggcgcgc accccggact
     5341 ggccttcgag ctcctcggcg gcctcggtgg cctgctgctc gacccgggcg aggccgtcgg
     5401 cggcccggcg cagggcagcg gcggtctgct ccgccgcctg gcgagcgtcg cccgcggtgc
     5461 gcttgagctc ggcgaagcgg gtgcgggcag catcccgggt ggcggtggcc gcggtgacgg
     5521 cctcggcggc ggcgtcgacc cgggtgcggg cctcctccag cgcagcgccg gtggcgccgg
     5581 tggccgagcc accgacccgc caggcaccgg caccgaagcg ggcaccgtcg gcggtgacga
     5641 cgacggcgtc ggggtgggcg agggcgacgt cgaggccggc ggcccagtcg ccgtcgatgc
     5701 gcacggcggc accgaccacg gcgtcgagga gggcgtcgac cccggggcgg ttggagcgca
     5761 cgtgactgcg cacgggctcg cccacgggcg gggcggtgcg ggcggtgcgg tcgaccccga
     5821 gggcgagcac ggcaccggtg gcgtcgccgg cggtgagcgc ggcgagggcc tggcgggcag
     5881 cgccggcacc gtcgacgacc acggcggaca gggcgtcggc ggcggcggcc tcgaaggcgc
     5941 tctcccagcc ggcgtcgacc tcgaccaggt cgagcagggt gccgaccacg ccgtcgacct
     6001 cggccaggcg ctcggcgccg gcggcggacc gggcctggtc caacgccagc tcgagcgcct
     6061 cggcgcgcgc cgaccaggag cggcgctcgg cgtcggcctc ggccagggca gcggcggcgt
     6121 cctcgacctc ggcttcggcg gcggctcgtt cggccagcag ccgatcgcgc tcggcctcga
     6181 gctcggggag gcgggtctcg gcttagcgga cctcgctgcg gaggcgctcg gcctcggcgg
     6241 tgaggcggtc gcgcttgtcg gcgagcgacc cggtgcggcc gtcggtgcgc tcgagctcgg
     6301 ccagcgagcg gctgacggcc tggcgcagcc cgcccagctg ccctcgcacc tcggcggcct
     6361 caccggaggg tgaggcgatg ccctcacccc agcgctcctc gaaggtggcg cgggcgtcgg
     6421 ccaggccgcg ctcggcgcgg tcgacctcct cggcctcggg caggagctcg gcggcggtgg
     6481 actcgatggt ggccagctcg tcgcgcaggc gggcctggtc ggcctcgagc gaggcgatca
     6541 gcgcggcgtc gacggtggac tcgcgctcct tggccaggct gcgctggcgc tcggccagca
     6601 cggcggcgat gccgcgaccg cgctcgcgca gggcctcgta gcgggtgagc tcgtcaccca
     6661 cgtcggcgcc gcccatggcg ttgagctcgg cctcggagcg cagcaccgac gcgtcgaggt
     6721 cgcggaggcg ggcccggagg gtctcctcgg tgccggcgtg ctgggcccgg tcgtgggtgg
     6781 tggtgtcgag gcggctacgc aggcgggcca gctcgcggcc ggcgaggaac cggcgcaggg
     6841 cgtcgagctc ggtgaccagg tcgccgtggc gacgggcggc ctcggcttgg cgctcgagcg
     6901 ggcgcaggcc acggcggacc tcgcggagca ggtcctgcac gcgggtgagg ttggcctcgg
     6961 tggaggccag gcggcgctcg gccttctcct tgcgcttgcg gaacttgagg acgccggcgg
     7021 cctcctcgat gatcagccgc cgctcggtgg gcttggcgtt gaggacggcg tcgatctggc
     7081 cctggctgat gatcacgtgc tgcatccggc ccacgccgga gtccgacagc agctcctgga
     7141 tgtcgagcag gcggcagggc acgccgttga tcgagtactc cgactccccc gtgcggaaga
     7201 gcgtgcgggt gatggtgacc tcggagaagt cgatcggcag caggccggcc gtgttgtcga
     7261 gggtcagcgc cacctcggcc cggccgagcg cgctgcgctt ggacgtgccg gcgaagatga
     7321 cgtcgtccat cttctgggac cgcaccgacg a
//
LOCUS       DATISU010000308         3609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_487000, whole genome shotgun sequence.
ACCESSION   DATISU010000308 DATISU010000000
VERSION     DATISU010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487000"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VLR27_12250"
     CDS             <1..609
                     /locus_tag="VLR27_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSP04271.1"
                     /translation="PAFGLGQVDPAEQQAFGVERTERAKRFDEALPLLRRFWTEDAVD
                     HDGTFFRYEGARVRPRPVQEPIEIWLGGVAPSELRRTGRLGDGWLPSFCTPDDVAAGR
                     PEIEHIAAEHDREIDPEHVGALVPYALDGIPDVLAAFLAKRRPDVDVRDVVAPSIDAL
                     VPLLERFVEAGASKFVAVPIGEPADWSAHLAEVADAVMPLQT"
     gene            654..1520
                     /locus_tag="VLR27_12255"
     CDS             654..1520
                     /locus_tag="VLR27_12255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HSP04272.1"
                     /translation="MKFGIIFANTGPFANGDAAAEMAVAAEEAGFESLWTVEHVVVPS
                     GYDSPYPYDPSGKMPGGEDFDIPDPLIWLSWVASATSTIRLATGILIVPQRNPVVLAK
                     ELATLDHLSGGRMELGIGVGWLEEEFDAIGVPFAERGKRTDDNIAAMRSLWSEEKATY
                     HGEFTSFDECISRPRPVNGSIPVHVGGHTDIAARRAGRLGDGFFPGKGTPQELARSFD
                     LVRETAREHGRDPEAIEFTAGGATIGSGALQAAKELADVGVDRVMVPAFAFWGDTADA
                     LKRYGDEVIANA"
     gene            complement(1574..2992)
                     /locus_tag="VLR27_12260"
     CDS             complement(1574..2992)
                     /locus_tag="VLR27_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04273.1"
                     /translation="MSDLPPPPPPPGRPGYPAGPPVAPPGGSPPAPPPSAPSSSWTPD
                     EEPRAPRGRRVSIVLAIGAVLALLAATIVVITRDGDDPQPAAAPSTTAPAGGEDDEPP
                     ANPTTTEPAGPPLSQDELEVLVEELQAFVADARGLEFLEPVEVELADGDAFQSRLLED
                     FEEDADQIADVEVFYKALGLLDPGADLLEELRAIYSAGVLGFYDPETNELVVRGTSLT
                     PYVRKTIVHELVHALDDQHFELDREEYEDRKDEIATGFTAVVEGNARRIEQLWIDEQP
                     DDVQDQAAAEERAFADGIDVDAFPEILLFEIGAPYELGKIFVDAIVAERGDRAVDAAL
                     EDPPTTSEQVLFPPLYSERQPRVEVPPPPADGEIVDDGVVGTLFWFGLFTTGDTTVPP
                     QDAFAAIQGWGGDWAVTWMDGDAACARIDVVGDTQQDTDELEDALRAWAEGSGAAEIT
                     VVDERVRVDSCVAGAGAVPPQV"
     gene            3101..>3609
                     /locus_tag="VLR27_12265"
     CDS             3101..>3609
                     /locus_tag="VLR27_12265"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HSP04274.1"
                     /translation="MARISTRITDLLGIDVPIIQAPMGWIARSQLASAVSEAGGLGII
                     ETSSGELDNVKAEIAKMRDLTDKPFGVNIAQAFVRDPGIVDFVVEQGVRFVTTSAGDP
                     TKYTAQLKDAGLTVFHVVPTWQGALKALDAGVDGLVVEGGEGGGFKNPRDVASMVLLP
                     LVAQHTDVP"
BASE COUNT          487 a         1331 c         1292 g          499 t
ORIGIN      
        1 ccggccttcg ggctcggcca ggtcgacccc gccgagcagc aggccttcgg cgtcgagcgc
       61 accgagcggg cgaagcgctt cgacgaggcc ctccccctgc tccggcgctt ctggaccgag
      121 gacgccgtcg accacgacgg caccttcttc cggtacgagg gtgcccgggt ccggccccgc
      181 cccgtccagg agccgatcga gatctggctc ggcggcgtcg ccccgtcgga gctgcgccgc
      241 accggccggc tgggcgacgg ctggctaccg agcttctgca ccccggacga cgtcgccgcc
      301 ggccgccccg agatcgagca catcgccgcc gagcacgacc gggagatcga ccccgagcac
      361 gtcggggcgc tggtgcccta cgcgctcgac ggcatccccg acgtcctcgc cgccttcctc
      421 gccaagcgcc ggcccgacgt cgacgtccgc gacgtggtcg cccccagcat cgacgcgctg
      481 gtccccctgc tcgagcggtt cgtcgaggcc ggcgcgtcca agttcgtcgc cgtgcccatc
      541 ggcgagccgg ccgactggtc ggcccacctc gccgaggtcg ccgacgccgt catgcccctg
      601 cagacctgat cgctacctga ccgaccggtc aagttcggtt acggtccggc gccatgaagt
      661 tcgggatcat cttcgccaac accgggccct tcgccaacgg tgacgccgcc gccgagatgg
      721 cggtcgccgc cgaggaggcc gggttcgagt cgctctggac cgtcgagcac gtcgtcgtgc
      781 cgtcgggcta cgactcgccc tacccctacg acccgtcggg gaagatgccc ggcggcgagg
      841 acttcgacat ccccgacccg ctgatctggc tcagctgggt cgcgtcggcc acctccacca
      901 tccggctggc cacgggcatc ctcatcgtcc cccagcgcaa cccggtggtg ctggccaagg
      961 agctcgccac cctggaccac ctctccggcg gccgcatgga gctgggcatc ggcgtgggct
     1021 ggctcgagga ggagttcgac gccatcggcg tgccgttcgc cgagcgaggc aagcgcaccg
     1081 acgacaacat cgccgccatg cggtccctgt ggagcgagga gaaggccacg taccacggcg
     1141 agttcaccag cttcgacgag tgcatctccc gcccccgccc cgtcaacggc tcgatccccg
     1201 tgcacgtcgg cggccacacc gacatcgccg cccgccgggc cggccgtctc ggcgacgggt
     1261 tcttccccgg caagggcacc ccgcaggagc tggcccgcag cttcgacctg gtgcgcgaga
     1321 ccgctcgcga gcacggccgc gacccggagg ccatcgagtt caccgccggc ggcgccacca
     1381 tcggcagcgg cgcgctgcag gcggccaagg agctcgccga cgtgggcgtc gaccgcgtga
     1441 tggtccccgc cttcgccttc tggggcgaca ccgccgacgc gctcaagcgc tacggcgacg
     1501 aggtcatcgc caacgcctga cggtgtgacg aacgggctgc cagttcggaa gaaccgttct
     1561 ggcagccctc ctgtcacacc tggggtggga cggcgccggc gccggccacg cagctgtcga
     1621 cgcgcacgcg ctcgtcgacg acggtgatct cggcggcccc gctgccctcg gcccaggccc
     1681 gcagggcgtc ctcgagctcg tcggtgtcct gctgggtgtc gccgaccacg tcgatgcggg
     1741 cgcaggcggc gtcgccgtcc atccaggtga cggcccagtc gccgccccag ccctggatgg
     1801 cggcgaaggc atcctggggc ggcacggtgg tgtcgccggt cgtgaacagc ccgaaccaga
     1861 acagggtgcc gacgaccccg tcgtcgacga tctcgccgtc ggcgggcggc ggcgggacct
     1921 cgacccgtgg ctggcgctcg ctgtacagcg gggggaagag cacctgctcc gacgtcgtcg
     1981 gcgggtcctc cagggcagcg tcgacggccc ggtcgccgcg ctcggcgacg atggcgtcca
     2041 cgaagatctt gcccagctcg tagggagcgc cgatctcgaa gagcaggatc tccgggaagg
     2101 cgtcgacgtc gatgccgtcg gcgaaggcgc gctcctcggc ggcggcctgg tcctggacgt
     2161 cgtcgggctg ctcgtcgatc cacagctgct cgatgcgccg ggcgttgccc tcgaccacag
     2221 cggtgaagcc agtggcgatc tcgtccttgc ggtcctcgta ctcctcccgg tccagctcga
     2281 agtgctggtc gtcgagggcg tgcaccagct cgtgcacgat ggtcttgcgg acgtaggggg
     2341 tcagcgacgt gccccgcacg accagctcgt tcgtctcggg gtcgtagaag ccgagcacgc
     2401 cggcggagta gatggcccgc agctcctcga gcaggtcggc gccggggtcg agcaggccga
     2461 gcgccttgta gaagacctcg acgtcggcga tctggtcggc atcttcctcg aagtcctcga
     2521 gcaggcggga ctggaaggcg tcgccgtcag ccagctcgac ctcgaccggc tccaggaact
     2581 ccaggccccg ggcgtcggcg acgaacgcct gcagctcctc gaccaggacc tccagctcgt
     2641 cctgggacag cggcggcccg gccggttcgg tggtggtcgg gttggcgggc ggctcgtcgt
     2701 cctccccgcc cgcgggcgcg gtggtcgacg gcgcggcggc gggctgcgga tcgtcgccgt
     2761 cacgagtgat gacgacgatg gtggccgcga gcagggcgag gacggcaccg atggccaaca
     2821 cgatgctgac ccggcggccg cgcggggccc ggggctcctc gtccggggtc cagctcgagg
     2881 acggcgccga cggtggcggc gccggcgggg acccacccgg gggtgcgacc ggcgggccgg
     2941 cggggtagcc ggggcggccg ggcgggggtg gcggtggagg cagatcggac acgtgcggtt
     3001 cccagggggt gtggaccgga gcgaacggac gacggtaccg ggtcactgct gcggtcccgc
     3061 cgtcaccacg acctcgccgg cgcccctacg ctccggcgcc atggcccgca tctccacccg
     3121 catcaccgac ctcctcggca tcgacgtccc gatcatccag gccccgatgg gctggatcgc
     3181 ccgctcgcag ctggcctcgg cggtgtccga ggccgggggt ctcggcatca tcgagacgtc
     3241 gtcgggcgag ctggacaacg tcaaggccga gatcgccaag atgcgggacc tgaccgacaa
     3301 gcccttcggc gtgaacatcg cccaggcgtt cgtgcgcgac cccggcatcg tggacttcgt
     3361 ggtcgagcag ggcgtgcggt tcgtgaccac ctcggccggc gaccccacga agtacaccgc
     3421 gcagctcaag gacgccgggc tcaccgtatt ccacgtggtg cccacgtggc agggtgcgct
     3481 caaggcgctc gacgccggcg tcgacggcct ggtcgtggag ggcggcgagg gtggcgggtt
     3541 caagaacccg cgcgacgtgg cctcgatggt cctgctgccg ctcgtggccc agcacaccga
     3601 cgtgccgat
//
LOCUS       DATISU010000309         3915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_68166, whole genome shotgun sequence.
ACCESSION   DATISU010000309 DATISU010000000
VERSION     DATISU010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68166"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VLR27_12270"
     CDS             <1..576
                     /locus_tag="VLR27_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04275.1"
                     /translation="PEEERPVKIDFEVGDFDSAEGDWPITVVNVSGDLEPDEVVVVEL
                     RDGDGEIVWAGEGVFSPPTTSVTVDSFVAVGDVDEAAIGQRMPEETTTTTAPPEPVVA
                     GNVVTNPTTTPPPSSTTSPPTTTEAIEPPAPPPTVEGDVTATPEPELPQQGSGLGGAG
                     QLALSMVIAVVFVAILFRAPLPAATTQRWRR"
     gene            573..1472
                     /locus_tag="VLR27_12275"
     CDS             573..1472
                     /locus_tag="VLR27_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04276.1"
                     /translation="MKRFLASLIISGFALLAMASPAAAAETLDVEAEINGRDISGADD
                     SSPIELDPTQAVPISITLRNAGSQTEEVRFVRLEGQALGLTFLTYDLGMRTSLAPGEE
                     TTFEAELDFFDLDEQATGYLGTSLRVYDADRLLLGQEDFVLDVKGRTTSTLGLFAFAV
                     LGIAGFSITVLVLNTLRRRLASNRFVRGLQFAVAGAAVGVTLALGLAILRIDYANAEE
                     WLPLVFLPTVIAFGIGYLAPGPLSLSIRDVREEEALQALAEEALIRRSGEQARATSTS
                     VFIDVHPTSQPSIDERTQEPAQR"
     gene            1646..2977
                     /locus_tag="VLR27_12280"
     CDS             1646..2977
                     /locus_tag="VLR27_12280"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSP04277.1"
                     /translation="MIDTFEPNELEAALNALDDAVGLVQSASLDGVSPKEELALVRRL
                     EELRRRLDHGTDQAVGHLDAGAAFSIDGHKTAKGALKAIGRLSGPEAHGRVQTSRALR
                     RLPLVEAAYARGEIPTEHARALARTVANPRVAEWVPDADPIFAHHATKLGYDDFVAAL
                     RQWEALADADGADQASERSHQRRNASLLESGIDGSFHLEGSFGAFQGAVMAEIFERYE
                     TAELHADWAAAREVHGEDACVEHLARTPGQRRADALVEIFRRAETAAGPSGEPLVNIV
                     VDQDTFEDEVRRAAGEDVTVDPNEDLTARRCQAVDGTQLRPSEVLAAAVLGHVRRVVL
                     DSAGNVIDLGRRQRLFTGSARDAAFLQALLRSPGGLGCLWPGCDGSGRCLQVDHRDPA
                     RQRGPTDVANSDAYCGFHNRIKERGFRPVRDPDGTWTIHRPDGQGPTTPPV"
     gene            complement(2979..>3915)
                     /locus_tag="VLR27_12285"
     CDS             complement(2979..>3915)
                     /locus_tag="VLR27_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSP04278.1"
                     /translation="RECDGVLIADGQASRRHANLAPRGETVVVEDLGSTNGTHLDGNR
                     ISSAVVLMPGAVLQVGDTTLQLVRSAPEAVQPAPAAPFGARETTVGGASSGGGSFGGA
                     VKAGPSGPQNARETSMDAVARAVEQAPSAIAAVEHDHGTITIVFSDIENSTSRATSMG
                     DAAWMKVLGEHNRIISRHVEKWNGTVVKNQGDGFMLTFNGARQALRAMIEVQRELTEL
                     EQRDPERGVRIRVGVHTGEVIAEEGDIFGKHVMLAARVGGLADGGEIVVSSIVKEIAS
                     ARGDLEFGPPQVVALKGIDGEHVVYPFLWQSFDDS"
BASE COUNT          583 a         1430 c         1338 g          564 t
ORIGIN      
        1 ccggaagaag aacgccccgt caaaatcgac ttcgaggtcg gcgacttcga ctcagccgag
       61 ggcgactggc cgatcaccgt cgtgaacgtg agcggcgacc tggagccgga tgaggtcgtc
      121 gtcgtcgagc tgcgggatgg cgacggcgag atcgtgtggg cgggtgaggg cgtgttcagc
      181 ccaccgacca cgtcggtcac cgtcgactcc ttcgtcgccg tcggcgacgt cgatgaggca
      241 gcgatcgggc agcggatgcc cgaggagacc acgacgacca ccgctcctcc ggaaccggtg
      301 gtcgctggga acgtggtcac gaacccgacg acgacgcctc caccgtcgag caccacgtcg
      361 cctcccacga cgaccgaagc gatcgaaccc ccagcgccac caccgacggt ggagggtgat
      421 gtcactgcca cgcccgagcc cgagctccca cagcagggca gcggcctcgg cggtgcaggc
      481 cagctcgctt tgtccatggt gatcgccgtc gtcttcgtcg ccatcctctt ccgggcgccg
      541 ctccctgcgg caacgaccca acggtggcga cgatgaagcg cttcctggcc agcctgatca
      601 tctccggctt cgcgctcctc gcaatggcca gcccggccgc ggcagctgag accttggacg
      661 tcgaggcaga gatcaacggc cgcgacatct ctggtgccga tgacagctcc cccatcgagc
      721 tcgatccgac ccaggccgtg ccgatcagca tcaccctgcg caacgccggc agccagaccg
      781 aggaggtccg cttcgtgcgg ctggagggcc aggcgctcgg gctcacgttc ctcacgtacg
      841 acctcggcat gcgcacgtcg ctggctcccg gggaggagac gaccttcgag gcagagctgg
      901 acttcttcga tctcgatgag caggcgacgg ggtacctggg cacatcgctg cgggtgtacg
      961 acgccgaccg gctgctgctc ggccaggagg acttcgtcct cgacgtgaag ggccgcacca
     1021 cgtcgacact ggggctcttc gcgttcgcgg tgctcggcat cgccggcttc agcatcaccg
     1081 tgcttgtcct caacaccctg cgccgacggc tggcgtccaa ccgattcgtg cgcgggctgc
     1141 agttcgccgt ggccggcgcc gcagtgggtg tgaccctggc cctcggcctc gccatcctgc
     1201 gcatcgacta cgcgaacgcc gaggagtggc tcccactggt cttcctaccg acggtcatcg
     1261 ccttcggcat cggctaccta gcccccggac cgctgtccct cagcatccgg gacgtgcgcg
     1321 aggaggaagc cctgcaggcc ctcgccgagg aggcgctcat ccgtcgctcc ggcgagcagg
     1381 cgcgtgcgac gagcaccagc gtgttcatcg atgtgcaccc cacgtcgcag ccgtcgatcg
     1441 acgagcgcac gcaggagccc gcccagcgct gatcgggcac gcgttcctgt cagaccccct
     1501 gagttgaatg gtgggtaggc gctccggctg gggtcggagt cgccactcgt cggccacagg
     1561 ccgaccgtcg acaccaccag ggaaggagcc gcgcgatgcg cgaccaccga ccacgtcgtg
     1621 gtgctgcctc agggatggcg gcgccatgat cgacacgttc gagcccaacg agctcgaggc
     1681 cgcgctgaac gcactggacg acgccgtcgg cttggtgcag tcggcgtcgc tggacggcgt
     1741 ctccccgaag gaggagctgg ctctggtccg ccgcctcgag gagctgcgcc gtcggctgga
     1801 ccacggcacc gatcaagccg tcgggcacct cgatgcgggc gccgcgttca gcatcgacgg
     1861 tcacaagacg gccaagggcg cgttgaaggc catcggacgg ctgtcgggtc ccgaggccca
     1921 cggcagggtg cagaccagcc gggccctgcg ccgcctgccg ctcgtcgagg cggcgtacgc
     1981 ccgaggcgag atccccaccg agcacgcgcg tgccctcgcc cgcaccgtgg cgaacccgcg
     2041 ggtggccgag tgggtgcccg acgccgatcc gatcttcgcc caccatgcca ccaagctcgg
     2101 ctacgacgac ttcgtggcgg ccctgcgcca gtgggaggca ctcgccgacg ccgacggcgc
     2161 cgaccaggca tccgagcggt cccaccagcg gcgcaacgcc tcgttgctgg agtccgggat
     2221 cgatggctcg ttccacctcg agggcagctt cggcgccttc cagggcgcgg tcatggccga
     2281 gatcttcgag cggtacgaga ccgccgagct ccacgccgac tgggccgccg cccgggaggt
     2341 ccacggcgag gacgcctgcg tcgagcacct cgcccgcacc ccgggccagc gccgcgccga
     2401 cgccctggtc gagatcttcc gtcgcgccga gaccgcagcg ggaccatcag gggagccgct
     2461 ggtcaacatc gtcgtcgacc aggacacctt cgaagacgag gtccgtcgag ccgccggtga
     2521 ggacgtgacg gtggatccga acgaggacct caccgcccgc aggtgccagg ccgtcgacgg
     2581 cacccagctc cggccctccg aggtgctcgc cgccgccgtg ctgggtcacg tccggcgggt
     2641 cgtgctcgac tcggccggca acgtcatcga cctcggtcgc cggcagcgcc tcttcaccgg
     2701 gtccgctcgg gacgcagcgt tcctccaggc cctgctccgg agcccgggag gcctcggctg
     2761 cctctggccc ggctgcgacg gctcgggacg gtgcctccag gtggaccacc gcgaccctgc
     2821 gaggcagcga ggaccgaccg acgtcgccaa ctccgatgcg tactgcgggt tccacaaccg
     2881 catcaaggag cgcggcttcc ggccggtgcg cgaccccgac ggcacctgga ccatccaccg
     2941 acccgacggt caggggccca ccacgccacc ggtgtgactc agctgtcgtc gaagctctgc
     3001 cagaggaacg ggtacacgac gtgctcgccg tcgatcccct tcagcgcgac cacctgcggg
     3061 gggccgaact cgaggtcgcc gcgcgccgag gcgatctcct tcacgatcga cgacaccacg
     3121 atctcgccgc cgtcggccag accgccgacg cgggcggcga gcatgacgtg cttgccgaag
     3181 atgtcgccct cctcggcgat gacctcgccg gtgtgcacgc cgacccggat gcgcacgccc
     3241 cgctcggggt cacgctgctc gagctcggtc agctcgcgct ggacctcgat catcgcccgg
     3301 agtgcctggc gagcgccgtt gaacgtgagc atgaagccgt cgccctggtt cttgacgacg
     3361 gtgccgttcc acttctccac gtgccggctg atgatccggt tgtgctcccc gagcaccttc
     3421 atccaggcgg catcacccat cgacgtggcc cgcgacgtgg agttctcgat gtcgctgaag
     3481 acgatggtga tggtgccgtg gtcgtgctcg acggcagcga tggcggacgg cgcctgctcg
     3541 accgcgcggg cgacggcgtc catcgaggtc tcgcgcgcgt tctgtggccc cgacgggccg
     3601 gccttcacgg cgccgccgaa gctgccgccg ccgctgctgg caccaccgac cgtcgtctcc
     3661 cgggcaccga agggcgctgc aggggcgggc tgcacggcct cgggtgcgga gcggacgagc
     3721 tgcagcgtgg tgtcgcccac ctgcagcacg gcgccgggca tcagcacgac ggcagaggag
     3781 atccggttcc cgtcgaggtg ggtgccgttg gtgctgccca ggtcctcgac cacgacggtc
     3841 tcaccgcgag gcgcgaggtt ggcgtgccgg cgggaggcct ggccgtcggc gatgaggacg
     3901 ccgtcgcact cccgg
//
LOCUS       DATISU010000310         1688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_21911, whole genome shotgun sequence.
ACCESSION   DATISU010000310 DATISU010000000
VERSION     DATISU010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21911"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VLR27_12290"
     CDS             complement(<1..818)
                     /locus_tag="VLR27_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HSP04279.1"
                     /translation="MSSDDIYILGIHMTKFGKHPDSDVVDLGAEAAMAALADAGVTMA
                     DMGILAAGNLMNASAGFGQQLQKQIGQTGIPVFNVANACATGATALRTAIMAIKAGEC
                     DMGLAVGVEKLAGAGLLGGGGGGKKSDEHKWEPSGRFGAVAGVDGRYGTAAMPGTFAQ
                     VGMEYLYEHQYAGEAFELFAKISVKNHKHSTLNPLAAYTKEMSLEQVMGDVMIAYPNT
                     RPMCSANCDGGAAAVVVSGAKLKTLSKEQQRRAVKVSASVLTSDPWQEACQVLP"
     gene            complement(830..1231)
                     /locus_tag="VLR27_12295"
     CDS             complement(830..1231)
                     /locus_tag="VLR27_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HSP04280.1"
                     /translation="MATKTQIPIVDYLVLGDDPHLEATECTSCGARYFDRRNACASCF
                     GTDFTTVDVPTEGTLRSFTIVTFAAPGVPVPFVAGIVDAGGTSVRTNIINVEPDDEHV
                     KLGMPVRLATYSLGTDDDGVEAIGFGYEPKA"
     gene            complement(1289..>1688)
                     /locus_tag="VLR27_12300"
     CDS             complement(1289..>1688)
                     /locus_tag="VLR27_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HSP04281.1"
                     /translation="VVRYASPVVFMRRTATEDVTLAGRDFSAGERFVMFYGAANRDPA
                     HFENPEVFDVRRDPNPHVGFGGPGPHFCLGAHLARRELAVAWRQLLTRLPDLEAAGPP
                     EYLEAAGIPLVGGIKRFPVRFTPTAPVGRA"
BASE COUNT          235 a          566 c          622 g          265 t
ORIGIN      
        1 tcgggcagca cctggcaggc ctcctgccag ggatccgacg tcagcaccga cgccgagacc
       61 ttgacggccc ggcgctgctg ctccttgctc agcgtcttga gcttcgctcc ggagaccacg
      121 accgcggccg cgccgccgtc acagttggcc gagcacatcg ggcgggtgtt cgggtaggcg
      181 atcatcacgt cgcccatcac ctgctcgagg ctcatctcct tggtgtacgc agcgagcgga
      241 ttcagcgtgg agtgcttgtg gttcttcacg ctgatcttgg cgaacagctc gaaggcctcg
      301 cccgcgtact ggtgctcgta caggtactcc atgccgacct gggcgaaggt gcccggcatg
      361 gccgcggtgc cgtagcggcc gtcgacgccg gcgacggcac cgaagcggcc cgagggctcc
      421 cacttgtgct cgtcggactt cttgccgccg ccaccgccgc cgaggaggcc ggcaccggcc
      481 agcttctcga cgccgacggc gaggcccatg tcgcactcgc cggccttgat ggccatgatc
      541 gccgtgcgca gcgcggtggc gcccgtggcg cacgcgttgg ccacgttgaa caccgggatg
      601 cccgtctggc cgatctgctt ctggagctgc tggccgaagc cggcgctcgc gttcatcagg
      661 ttgccggcgg ccaggatgcc catgtcggcc atggtcacgc cggcgtcggc gagggccgcc
      721 atggccgcct ccgcgccgag gtcgaccacg tcggagtccg ggtgcttccc gaacttcgtc
      781 atgtggatgc cgaggatgta gatgtcgtcg ctgctcatgt cgttgccccc taggccttcg
      841 gttcgtagcc gaagccgatg gcctcgaccc cgtcgtcgtc ggtgccgagc gagtaggtcg
      901 ccaggcgcac cggcatgccg agcttcacgt gctcgtcatc gggttcgacg ttgatgatgt
      961 tggtccgcac gctggtgccg ccggcgtcga cgatgccggc cacgaagggc acgggcaccc
     1021 cgggagcggc gaaggtcacg atggtgaacg agcgcagcgt gccctcggtg ggcacgtcga
     1081 ccgtggtgaa gtcggtgccg aagcacgacg cgcacgcgtt gcgccggtcg aagtagcgcg
     1141 ccccgcacga ggtgcactcg gtggcctcca ggtgcgggtc gtcgcccagc accaggtagt
     1201 ccacgatcgg gatctgggtc ttcgttgcca cggagctcct gtcagatggg tggccgcgcg
     1261 tccccggcgc gaccgtgatg gtgggaatct aggcccgtcc caccggggcg gtgggcgtga
     1321 accggacggg gaagcgcttg atgccaccca ccagcgggat gccggcggcc tcgaggtact
     1381 cgggcggacc ggccgcctcg aggtcgggca ggcgggtgag cagctggcgc caggccacgg
     1441 cgagctcgcg acgggccagg tgcgcgccga ggcagaagtg cgggccggga ccaccgaagc
     1501 cgacgtgggg gttcgggtcg cggcgcacgt cgaagacctc ggggttctcg aagtgggccg
     1561 ggtcgcggtt ggcggcgccg tagaacatga cgaagcgctc gccggcggag aagtcccggc
     1621 cggccagggt gacgtcctcg gtggccgtgc ggcgcatgaa caccaccgga gaggcgtagc
     1681 gcacgacc
//
LOCUS       DATISU010000311         4216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_301414, whole genome shotgun sequence.
ACCESSION   DATISU010000311 DATISU010000000
VERSION     DATISU010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301414"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /gene="doeB2"
                     /locus_tag="VLR27_12305"
     CDS             complement(<1..1080)
                     /gene="doeB2"
                     /locus_tag="VLR27_12305"
                     /EC_number="3.5.1.125"
                     /inference="COORDINATES: protein motif:HMM:NF038260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(2)-acetyl-L-2,4-diaminobutanoate deacetylase
                     DoeB2"
                     /protein_id="HSP04282.1"
                     /translation="MQDATDLRRRLHQRPELGWSETWTAAAIRDELDRLGITWRVCAG
                     TGTVATLAAGAPGRHVALRADIDAMPVHEATGATWASQVDGMMHACGHDGHTATLLGT
                     ARWLRDHEDALPGPVTLLFQPAEEGGHGARGMIEDGALDGVDVVFGWHNWPAIPFGQA
                     VCPDGTVMCGNGTFEILVEGVGGHASQPEATRDPVLAAAAITVAIQQVVSRRLPPQQP
                     TVVAVTSIDAPSAATVTPASARLGGSIRIAEEAARDEVGALIDEVVHHVAAGYGCQAQ
                     VVHQRRYGPTINHPGPAAEVRAALADELGDGWHAAAAPVPIMASEDFSYYLTEVPGAF
                     ALVGADDGQGHDASCHSPHYDFNDRL"
     gene            complement(1169..1993)
                     /locus_tag="VLR27_12310"
     CDS             complement(1169..1993)
                     /locus_tag="VLR27_12310"
                     /inference="COORDINATES: protein motif:HMM:NF022740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 family protein"
                     /protein_id="HSP04283.1"
                     /translation="MRRPARRSMEPVDLDPRPRRYPVIGAVHGTPITHLPTGTVGVVE
                     RFLNSQLQMKDRTGYVHTFTVVPGEFAIDGVACHPVFGSAKPAEPERRISASGSIAVR
                     HEARVARASRILVEGIHDAELLEKIWGDDLRHEGIVVERLDGMDDLAAVVRDFRPGPT
                     RRLGVLLDHLVEGSKESRVAATVQHPHVLVTGTPYVDVWQAVRPSVAGIDAWPEVPMG
                     EPWKEGVLARIGRPGQHPGAFWRELLASVTTYADLFPPLVGAVEQLIDFVTEIEEG"
     gene            2138..3139
                     /locus_tag="VLR27_12315"
     CDS             2138..3139
                     /locus_tag="VLR27_12315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HSP04284.1"
                     /translation="MDNAPVGSANEPTGGGPEHVRALEAEVRRLRDQLDRLETSEAEW
                     RTLVETLPQIVWITRPDGWHEHFNRQWLEFTGLTLEESLGDGWNPPFHPEDRPLAWKL
                     WQEATATGTPYEIEYRLRRHDGVYHWMLGRALPLRGRDGNIVKWFGTCTDIDELKVAQ
                     AQVAGLVVELERRADHDPLTGLANRDLLFRQMELLLRQRHGVGVAAAFVDLDGFKAVN
                     DRCGHDAGDKLLLRVADQLRDAVRQGDVAARIGGDEFVVVGEADDEHDAERFAERLRA
                     ALGTSCRIGDEEIPVTASIGVVYVAPGDPASPDEVLSRADARMYRNKRQRRTGTSSG"
     gene            complement(3199..>4216)
                     /locus_tag="VLR27_12320"
     CDS             complement(3199..>4216)
                     /locus_tag="VLR27_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSP04285.1"
                     /translation="AIDHVQLAMPPGGEELATWFYEGILGVPRVPKPPHLAVRGGCWF
                     ERGALRIHLGVQDDFAPATKAHPAFTVLDLDRLEATITTHGLAVRRVDDPLEPDVLYV
                     DDPFGNRIELRPARGTEDKADYAEVVEGVARSAVGDRLADELAFVHELDRLKTVLRQS
                     FVGDASRRENTAEHSWHVAVMAAVLSEHLEADVDLARVVLLLLVHDIVEIDAGDTGIY
                     DHTSAGSKEDREVAAADRLYGLLPDDVGARLRDAWEEYEHGTTPEARAARVFDRLAPV
                     LLNLSAGGIAWRRWGVSADRVREVVAPIGAGSPDLGRFIEAALDQAVADGLLPAGGSG
                     LILP"
BASE COUNT          569 a         1462 c         1616 g          569 t
ORIGIN      
        1 cagtcggtcg ttgaagtcgt agtgcggact gtggcacgag gcgtcgtggc cctggccgtc
       61 gtcggcgccg accagggcga aggcccccgg cacctcggtg aggtagtagc tgaagtcctc
      121 cgacgccatg atcggcacgg gtgcggccgc ggcgtgccag ccgtcgccga gctcgtcggc
      181 gagggcggcc cgcacctccg cggccggacc ggggtggttg atcgtcggcc cgtagcggcg
      241 ctggtgcacg acctgggcct ggcagccgta ccccgccgcc acgtggtgca ccacctcgtc
      301 gatgagcgcg ccgacctcgt ctcgtgcggc ctcctcggcg atgcggatgc tgccgccgag
      361 ccgggccgat gccggggtga cggtggcggc gctgggcgca tcgatggacg tgaccgccac
      421 gacggtgggc tgctgcgggg gcagccgccg gctgaccacc tgctggatcg cgaccgtgat
      481 ggcggcggcg gcgagcacgg ggtcgcgcgt cgcctcgggc tgactggcgt gcccgccgac
      541 gccctcgacg aggatctcga aggtgccgtt gccgcacatg accgtgccgt cggggcacac
      601 cgcctggccg aacgggatgg ccggccagtt gtgccacccg aacacgacgt cgacgccgtc
      661 gagcgcaccg tcttcgatca tcccgcgggc cccgtggccg ccctcctcgg cgggctggaa
      721 caggagcgtc accggtccgg gcagcgcgtc ctcgtggtcc cgcagccagc gggcggtgcc
      781 gagcagcgtg gccgtgtgcc cgtcgtggcc gcaggcgtgc atcatgccgt ccacctgcga
      841 ggcccaggtc gcacccgtcg cctcgtggac cggcatggcg tcgatgtcgg cccgcagggc
      901 cacgtgccgt ccgggggcgc cggcggccag ggtggcgacg gtgccggtgc cggcgcagac
      961 ccgccaggtg atgccgaggc ggtcgagctc gtcgcggatc gccgctgccg tccaggtctc
     1021 gctccagccc agctccggtc gctggtgcag ccgacgtcgc aggtctgttg cgtcctgcac
     1081 gacggtgttc cagctggtgc ccactgcgct cccagatcgt tgctcggtgc cgggaccgta
     1141 gcgctccggg gcggctggtg atccccgatc agccctcttc gatctcggtg acgaagtcga
     1201 tgagctgctc gaccgcgccg acgaggggcg ggaacaggtc ggcatacgtc gtgacgctgg
     1261 cgagcagctc ccgccagaac gcgccggggt gctggcccgg gcggccgatg cgggcgagca
     1321 cgccctcctt ccacggctcc cccatcggga cctcgggcca ggcgtcgatg ccggcgaccg
     1381 acggccgcac cgcctgccac acgtcgacgt agggggtgcc ggtgaccagc acgtgggggt
     1441 gctgcacggt ggcggcgacg cgggactcct tggagccctc gacgaggtgg tcgagcagga
     1501 cgccgagccg ccgggtcggt ccgggccgga agtcgcgcac gaccgcggcg aggtcgtcca
     1561 tcccgtcgag gcgctcgacg acgatgccct cgtggcgcag gtcgtcgccc cagatcttct
     1621 cgagcagctc ggcgtcgtgg atgccctcca ccaggatgcg gctggcgcgg gcgacccggg
     1681 cctcgtggcg cacggcgatc gacccggacg ccgagatgcg ccgctcgggc tcggcgggct
     1741 tcgccgaacc gaagacgggg tggcaggcga cgccatcgat ggcgaactcg ccgggcacga
     1801 ccgtgaacgt gtgcacgtac ccggtgcggt ccttcatctg cagctgggag ttcaggaacc
     1861 gctcgacgac cccgacggtg ccggtgggca ggtgggtgat cggggtgccg tgcacggcgc
     1921 cgatgacggg gtaccggcgg gggcgcggat cgaggtcgac gggctccatc gatcggcggg
     1981 ccgggcggcg catggccccg agggtacgga atccccggac cacgatcggg gagggcgaaa
     2041 cggacgactg gggcggattg tctggctcga tcgtgtcacg cggagaaggg cgcaactgct
     2101 ctggcggacg gaccgtggac ctgtgcacga tcgcgggatg gacaacgcgc ccgtcggatc
     2161 ggcgaacgag ccgaccggtg gaggtccgga gcacgtgcgc gcgctcgaag cagaggtccg
     2221 ccgcctccgg gaccagctcg accgcctgga gaccagcgag gccgagtggc gcacgctcgt
     2281 cgagacgctg ccccagatcg tgtggatcac ccggcccgac ggctggcacg agcacttcaa
     2341 ccgccagtgg ctcgagttca ccggcctgac gctcgaggag agcctcggtg acggctggaa
     2401 cccgccgttc caccccgagg accgccccct cgcctggaag ctgtggcagg aggccacggc
     2461 caccgggacg ccgtacgaga tcgagtaccg gctgcgacgc cacgacggcg tctaccactg
     2521 gatgctcggc cgggcgctgc ccctgcgtgg ccgcgacggg aacatcgtca agtggttcgg
     2581 cacgtgcacc gacatcgacg agctgaaggt ggcccaggcg caggtcgccg ggctcgtcgt
     2641 cgagctggag cggcgggccg accacgaccc gctcaccggc ctcgccaacc gcgacctgct
     2701 cttccgccag atggagctgc tgctgcgcca gcgacatggc gtcggcgtcg ctgcggcgtt
     2761 cgtcgacctc gacggcttca aggcggtcaa cgatcgctgc ggccacgacg ccggcgacaa
     2821 gctcctcttg cgggtggccg accagctgcg cgacgccgta cgccagggcg acgtagcggc
     2881 ccggatcggc ggggacgagt tcgtggtggt gggcgaggcg gacgacgagc acgacgcgga
     2941 gcggttcgcc gagcgcctgc gtgcagccct cggcacgagc tgccgcatcg gcgacgagga
     3001 gatcccggtc acggccagca tcggggtggt ctacgtcgcc ccgggcgacc cggcgtcacc
     3061 cgacgaggtg ctctcccggg ccgacgcccg catgtaccgg aacaagcggc agcgcaggac
     3121 gggcacgtcg tcgggctgac gcgcgctgca cgggtactgc cagaacggga ggggtggact
     3181 gcctcgacct gcgtgtcgtc agggaaggat gaggccggag ccgccggcgg ggaggaggcc
     3241 gtcggcgacg gcctggtcca gggcggcctc gatgaagcgg ccgagatccg gcgagccggc
     3301 accgatcggg gcgacgacct cgcggacccg gtcggcgctg accccccagc gacgccaggc
     3361 gatcccgccg gcgctgaggt tcaacaacac gggcgcgagc cggtcgaaga cccgggcggc
     3421 tcgggcctcc ggggtggtgc cgtgctcgta ctcctcccag gcgtcgcgga ggcgggcgcc
     3481 gacgtcgtcg ggcagcaggc cgtagagccg atcggctgcg gccacctcgc ggtcctcctt
     3541 ggaccccgcc gaggtgtggt cgtagatgcc ggtgtccccg gcgtcgatct ccacgatgtc
     3601 gtggacgagc agcaggagga ccacgcgggc cagatcgacg tcggcttcga ggtgctcgct
     3661 cagcaccgcg gccatgactg cgacgtgcca gctgtgctcg gccgtgttct cccgccgtga
     3721 cgcgtcgccc acgaaggact ggcgcagcac ggtcttgagc cggtccagct cgtggacgaa
     3781 ggcgagctcg tctgcgagtc gatcgcccac tgcgctcctg gccacgccct cgacgacctc
     3841 ggcgtagtcg gccttgtcct cggtgccgcg agctggtcgc agctcgatgc ggttgccgaa
     3901 cgggtcgtcg acgtagagga cgtcgggctc cagcgggtcg tcgacccggc gcacggcgag
     3961 cccgtgcgtg gtgatcgtgg cctcgaggcg gtcgaggtcg agcacggtga aggccgggtg
     4021 ggccttggtc gccggggcga agtcgtcctg caccccgagg tggatgcgca gggcgccccg
     4081 ctcgaaccag cacccgcccc ggacggcgag gtgcggcggc ttcgggacgc ggggcacgcc
     4141 gaggatgcct tcgtagaacc aggtggccag ctcctcgccg ccgggcggca tggcgagctg
     4201 gacgtggtcg atcgcg
//
LOCUS       DATISU010000312         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_673604, whole genome shotgun sequence.
ACCESSION   DATISU010000312 DATISU010000000
VERSION     DATISU010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673604"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..569)
                     /locus_tag="VLR27_12325"
     CDS             complement(75..569)
                     /locus_tag="VLR27_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSP04286.1"
                     /translation="MADDQDIDLDLELDVPSRGTTPRVALVAEDEPGIRSLIVSLLTL
                     DGWEVHVTDTGENVADLIDQHHPVLCLLDVMMPGKDGVEAMEEGRSRGHGAPDGTVYV
                     ILTALAGQEDVIRGVVSGADDYITKPFDVDDLSDRVARWCEATGWELVDAEHPRVVHE
                     HLRY"
     gene            complement(671..1117)
                     /locus_tag="VLR27_12330"
     CDS             complement(671..1117)
                     /locus_tag="VLR27_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04287.1"
                     /translation="MSDTGGALASAPESRVVVDALGSVLDGDAAAARDLLVAGAEVTG
                     WASLLHRLDVAGRALALDAAIAPSAAPAWSVHVAALAASAGVDPLPAGTSEVLVAWSQ
                     GDGVPVQIDLTTDVVGAAPEAESAWAALLVLACLVDRSGFPPQVVA"
     gene            1256..2752
                     /locus_tag="VLR27_12335"
     CDS             1256..2752
                     /locus_tag="VLR27_12335"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSP04288.1"
                     /translation="MPRIAIIGSGFAGMGLGIQLKQQGIDTFTIFEKADAIGGTWRAN
                     TYPGAECDIPSALYSYSFENFDWPNKWSHQPVIKDYLEHCAEAYGLWAHIRLETPVTE
                     LRWVEEAGAWDVDATPVGGETTTERYDVVVSATGQLSRPRWPDIDGLDSFAGPVFHSA
                     EWDHSVDLTGKRVASIGAGASAVQYVPQIAKEASKVTIFQRTPNWIIPKNDRDYTEIE
                     KSLIRRSRVARNLRRFMIFARAELLGYNLMHQKSFVRDFMQKFAIKNLQEKVSDPDKV
                     AKLTPDYPMGAKRVLFSDDFYEAIEEHDVDVVTDRIDKVTGDAIVTVSGMDQDHVEHP
                     VDVIVVGTGFHTQDFLTPMTVIGPEGRNLNEQWQEGPQAHLGLAVTGFPNLFFMYGPN
                     TNLGHNSIVLMIESQCRYILSALRQMAARSIQSIEVKAEVQQAYNDELSRRLADSAWV
                     LIEDSWYLQGGKVTNNWVGRTTEYRRRTNHADLDEYHLRPKPALTPAS"
     gene            complement(2762..>3338)
                     /locus_tag="VLR27_12340"
     CDS             complement(2762..>3338)
                     /locus_tag="VLR27_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04289.1"
                     /translation="SLLAGDEFVGSALQDGADDQNPVPLDAAQAAAGAANDLDPGPVA
                     TVLIGVEADGEGIALPALEVRLPSDTLLVVEGVDHDGWTVVRGTPGMDEVVELPEQHA
                     TFIDAVEYVAAAVGKAGGSLPVVDTDEGIEAVAIFDADGGVYAVLKEAEDAYTFAWID
                     DDGDLERLDTYATLQEAILLPVLDEELFGGM"
BASE COUNT          523 a         1220 c         1126 g          469 t
ORIGIN      
        1 tcgagctccc gcacggcagc acggccccgc cgcttcgacc cgaccagctc cggcacgtcg
       61 tccaccacga ccggtcagta cctcaggtgc tcgtgcacga cgcgggggtg ctcggcgtcg
      121 acgagctccc agcccgtcgc ctcgcaccag cgggccaccc ggtcggagag gtcgtccacg
      181 tcgaagggct tggtgatgta gtcgtcggcg ccggacacga cgccgcggat gacgtcctcc
      241 tggccggcga gcgcggtgag gatgacgtag acggtgccgt cgggtgcgcc gtgcccgcgg
      301 gaccgaccct cctccatggc ctccacgccg tccttgcccg gcatcatcac gtcgagcagg
      361 cacaggaccg ggtggtgctg gtcgatgagg tcggcgacgt tctcgccggt gtcggtgacg
      421 tgcacctccc acccgtccag cgtcagcagc gagacgatga gcgaccggat gccgggctcg
      481 tcctcggcca ccagcgcgac gcggggtgtc gtgccccggg agggcacgtc gagttcgagg
      541 tcgaggtcga tgtcctggtc gtctgccatg tggggtgcac gtgggctcgc tccggccacg
      601 tgtgaccgac gctaccctcc ggcccccgac cccggcggtg tcggagggtg cgcctcccgg
      661 caggtgcgac ctacgcgacg acctgcggcg ggaagcccga gcggtccacc aggcacgcca
      721 gcacgagcag cgcggcccag gcgctctcgg cctcgggcgc ggcaccgacc acgtcggtgg
      781 tgaggtcgat ctgcacgggc accccgtcac cctggctcca ggccacgagg acctcggacg
      841 tgccggcggg cagcggatcg acgcccgccg aggcagcgag ggcggcgacg tgcacggacc
      901 acgccggtgc ggccgacggg gcgatggctg cgtcgagcgc gagggcgcga ccggcgacgt
      961 cgaggcggtg cagcagggag gcccagccgg tgacctccgc gccggcgacc agcaggtcgc
     1021 gggccgcggc cgcgtcgccg tccaggaccg atccgagggc atcgacgacg acgcgcgact
     1081 cgggcgcgct cgccagggca ccacccgtat cggacatctc ggtcacatgg catggttcgc
     1141 cgctggccga ggcacctcct accgcctgca tcgggcttcc cggacgtggc tgccggccgg
     1201 cgagcaggat acggtcgtta ccagaactcg tcatcatgtt ccagggggtc ccttcgtgcc
     1261 acgcatcgcc atcatcggct ccggcttcgc cggcatgggc ttgggcatcc agctcaagca
     1321 gcagggcatc gacacgttca ccatcttcga gaaggccgat gccatcggtg gcacctggcg
     1381 ggcgaacacc tacccaggtg ccgagtgcga catcccctcg gccctgtact cgtactcgtt
     1441 cgagaacttc gactggccca acaagtggtc ccaccagccg gtgatcaagg actacctcga
     1501 gcactgcgcc gaggcctacg ggctgtgggc ccacatccgc ctcgagacgc cggtgaccga
     1561 gctgcgctgg gtcgaggagg cgggcgcgtg ggacgtcgac gccaccccgg tgggcgggga
     1621 gaccaccacc gagcgctacg acgtggtcgt ctcggccacc ggccagctga gccggccccg
     1681 ctggcccgac atcgacggcc tcgactcctt cgccggcccg gtcttccact cggccgagtg
     1741 ggaccactcc gtcgacctca ccgggaagcg ggtggcgtcc atcggtgccg gcgccagcgc
     1801 cgtgcagtac gtgccccaga tcgccaagga ggcgtcgaag gtcacgatct tccagcgcac
     1861 cccgaactgg atcatcccga agaacgaccg cgactacacc gagatcgaga agtccctgat
     1921 ccgtcgcagc cgggtggccc gcaacctgcg tcggttcatg atcttcgccc gagccgagct
     1981 gctcgggtac aacctcatgc accagaagtc cttcgtgcgg gacttcatgc agaagttcgc
     2041 gatcaagaac ctgcaggaga aggtcagcga ccccgacaag gtggccaagc tgacccccga
     2101 ctacccgatg ggcgccaagc gggtcctgtt ctccgacgac ttctacgagg cgatcgagga
     2161 gcacgacgtc gacgtcgtca ccgaccgcat cgacaaggtc accggcgacg ccatcgtcac
     2221 cgtgtcgggc atggaccagg accacgtcga gcaccccgtc gacgtcatcg tcgtgggcac
     2281 cggcttccac acccaggact tcctcacgcc gatgaccgtc atcggacccg agggtcgcaa
     2341 cctgaacgag cagtggcagg agggcccgca ggcgcacctc ggcctcgcgg tcaccggctt
     2401 cccgaacctg ttcttcatgt acggccccaa caccaacctg ggccacaact cgatcgtgct
     2461 gatgatcgag tcgcagtgcc gctacatcct ctcggcgctg cgccagatgg cggcccggtc
     2521 gatccagtcg atcgaggtca aggccgaggt gcagcaggcc tacaacgacg agctctcccg
     2581 tcgcctggcc gacagcgcct gggtgctcat cgaggacagc tggtacctcc agggcggcaa
     2641 ggtcaccaac aactgggtgg gtcgcaccac cgagtaccgc cggcgcacca accacgccga
     2701 cctcgacgag taccacctgc gccccaagcc cgccctcacc ccggccagct gaccgctggc
     2761 ctcacatccc gccgaagagc tcctcgtcca ggacggggag caggatcgcc tcctggaggg
     2821 tggcgtaggt gtcgaggcgc tcgaggtccc cgtcgtcgtc gatccaggcg aaggtgtagg
     2881 cgtcctcggc ctccttcagc acggcgtaga cgcccccgtc ggcgtcgaag atcgccacgg
     2941 cctcgatgcc ctcgtcggtg tcgaccaccg gcagcgaccc tccggccttg ccgacggcgg
     3001 ctgccacgta ctcgacggcg tcgatgaagg tggcgtgctg ctcggggagc tcgacgacct
     3061 cgtccatccc cggcgtgccc cgcacgaccg tccagccgtc gtggtcgacc ccttcgacga
     3121 ccagcagggt gtcgctcggc aggcgcacct cgagcgccgg cagggcgatc ccctcgccgt
     3181 cggcctcgac gccgatcagg acggtggcca ccgggccggg gtcgaggtcg ttcgccgcgc
     3241 cggcggcggc ctgcgcggcg tcgaggggga cggggttctg gtcgtcggcg ccgtcctgga
     3301 gcgcagagcc cacgaactcg tcgccggcca gcagcgag
//
LOCUS       DATISU010000313         3672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_627032, whole genome shotgun sequence.
ACCESSION   DATISU010000313 DATISU010000000
VERSION     DATISU010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627032"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1245
                     /locus_tag="VLR27_12345"
     CDS             238..1245
                     /locus_tag="VLR27_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HSP04290.1"
                     /translation="MTSGESTETGTRMRAVVQHEYGGPETLHLGEIERPVPGAGQVLV
                     EVRAAGVERGVEHLRTGVPLLIRPVYGWRRPKNPVRGREAAGVVAEVGEGVTRFAVGD
                     EVFGVAEGTFAEYAVGEESKLAHLPAGMSFIDAAALPISGITALQGLLEVGGLEAGQD
                     VLVIGASGGVGSFAVQIAHATGATVTGVCSGAKADLVRSLGADHVIDHTSEDFATGDR
                     RYDLVFDIGGNSSLRRLRRVLRRDGTLVLVGGESAGGKLLAGFDRQLRAVALSPFISQ
                     RLTMFVAGEDHERLEQLASMVEQGQLRPAVGATYELADAPQAMRDLAAGRARGKLVVT
                     I"
     gene            complement(1255..1806)
                     /locus_tag="VLR27_12350"
     CDS             complement(1255..1806)
                     /locus_tag="VLR27_12350"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HSP04291.1"
                     /translation="MTTIRTVGHGTADADELGELLRGAGVTELVDVRRFPGSRRHPQF
                     SSEAMAAWLPEHEVGYQHLESLGGRRKLGPDSPHIGLRNEQFRAYADHMESDEFIDGV
                     AELLRIAENESVAVMCSESVWWRCHRRLLADHLVLVHDVVVEHLFHDGRLTRHDPTPE
                     ARLHDEHVIYDVGVAPPLFTPED"
     gene            complement(1803..3014)
                     /locus_tag="VLR27_12355"
     CDS             complement(1803..3014)
                     /locus_tag="VLR27_12355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HSP04292.1"
                     /translation="MTAPGRAELASLRRHLHRHPETAFDVDASAGLVAERLAAAGLDV
                     TRGVGGSGVVATLRRGTSSRSIGLRADLDALPIQEANTFDHRSQAPGAFHGCGHDGHT
                     TMLLGAALHLAADTGPDSSDIDGTVHFVFQPDEENGRGAQAMIDDGLLERFPMDAVYG
                     MHNLPGLAVGRFATRSGPFTAYEERFEIEIHGRGGHASAPERTIDPLVAGAHVVIGLQ
                     SIVSRALAPQDHGVVSVTELITDGAPNIIPTTVTIRGDVRGYEEHVSEAIRSRMGAIV
                     EGTAAAHGATATLRYARAFEPTVNTAAEVDVAARAVGGVAGAGIDTDHAPMGFSEDFA
                     QFLQHRPGCFILIGNGTEGCHGATLHNPHYDFNDDALPFGVAYWTALVRDVLATVAPP
                     PLDADGGGASR"
     gene            complement(3138..>3672)
                     /locus_tag="VLR27_12360"
     CDS             complement(3138..>3672)
                     /locus_tag="VLR27_12360"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSP04293.1"
                     /translation="VVHLDVESQIGSLHLGPALTEAERLYLTCDARVETWFERDGKPI
                     GVGRETREIPRRIRRALEHRQSTCAVPGCGATRGLHAHHIWHWEKGGPTELWNLVLVC
                     PFHHRLHHRKLITISGPGHAVTVTDVNGRTLDGSSVARPPTGPPPQAPPYAGPTGERA
                     DWKWYEPMAPGARGTDN"
BASE COUNT          487 a         1288 c         1388 g          509 t
ORIGIN      
        1 cgccgcagcg agtgcgagcg aggggacgag gaagcgacgc gtggacacgg cagtgccttt
       61 caggtgcggg cgcaccgttg cgccctgccg gtctctccgg cgcagatcag acgcagcagc
      121 cggcacttct gttcccgcac cgactgtggc ctgcgtcaca ccctgcgagc gcccgccagc
      181 atctgcctac ggtgatgccg aaatccacag cgcgacagcg acgggcaggg acgggccatg
      241 accagcggcg agtcgaccga gaccggcacg cgcatgcgcg ccgtcgtcca gcacgagtac
      301 ggcggaccgg agacgctcca cctcggcgag atcgagcggc cggtgcccgg tgccggccag
      361 gtgctggtcg aggtgcgcgc cgccggggtc gagcgcggcg tcgagcacct caggaccggc
      421 gtgcccctgc tgatccgtcc cgtctacggc tggcgccggc cgaagaaccc ggtgcggggc
      481 cgcgaggcgg cgggcgtcgt cgccgaggtg ggcgagggcg tcacccgctt cgccgtcggg
      541 gacgaggtgt tcggcgtggc tgaggggacc ttcgccgagt acgccgtcgg cgaggagtcc
      601 aagctggcgc acctgcccgc cgggatgtcc ttcatcgatg ccgcggcgct gcccatctcc
      661 ggcatcacgg cgctgcaggg cctgctcgag gtcggcggcc tggaggccgg ccaggacgtg
      721 ctcgtcatcg gcgcatccgg aggcgtgggg tccttcgccg tgcagatcgc ccacgccacg
      781 ggggccacgg tcaccggcgt gtgctcgggc gccaaggccg acctcgtccg ctcgctcggc
      841 gccgaccacg tgatcgacca cacgtccgag gacttcgcca cgggcgaccg gcgctacgac
      901 ctcgtcttcg acatcggcgg caactcgtcg ctgcgccggc tccggcgggt gctgcgccgg
      961 gacggcacgc tggtgctggt gggcggcgag agcgcgggcg ggaagctgct cgccggattc
     1021 gaccgccagc tgcgcgccgt cgccctctcc cccttcatca gccagcggct gacgatgttc
     1081 gtcgccggcg aggaccacga gcgtctcgag cagctggcat cgatggtcga gcaggggcag
     1141 ctgcgccccg ccgtcggcgc gacctacgag ctggccgatg caccccaggc gatgcgcgac
     1201 ctggccgcgg gccgggctcg ggggaagctg gtcgtcacga tctgaccggg cggctcagtc
     1261 ctccggcgtg aacagcggcg gggcgacgcc gacgtcgtag atcacgtgct cgtcgtggag
     1321 acgtgcttcc ggggtcgggt cgtggcgggt gagacggccg tcgtggaaca ggtgctcgac
     1381 gaccacgtcg tgcacgagca ccaggtgatc ggccagcagc cggcggtggc accgccacca
     1441 gaccgactcc gagcacatca ccgccacgct ctcgttctcg gcgatccgca ggagctcggc
     1501 caccccgtcg atgaactcgt cgctctccat gtggtccgcg taggcccgga actgctcgtt
     1561 gcgcagcccg atgtgcggcg agtcgggccc gagcttccgc cggccgccga gcgactcgag
     1621 gtgctggtag cccacctcgt gctcgggcag ccacgccgcc atcgcctcgg acgagaactg
     1681 cgggtggcgg cggctaccgg ggaagcggcg aacgtcgacc agctcggtga cgccggcgcc
     1741 gcgcagcagc tcgccgagct catcggcgtc ggccgtgccg tgcccgacgg tgcggatcgt
     1801 cgtcatcggg atgctccgcc accgtctgca tcgaggggcg gtggtgcgac cgtcgcgagc
     1861 acgtcgcgga cgagggccgt ccagtacgcg acgccgaacg gcagggcgtc gtcgttgaag
     1921 tcgtagtgcg ggttgtgcag cgtggcgccg tggcagccct cggtcccgtt gccgatcagg
     1981 atgaagcaac cgggccggtg ctggaggaac tgggcgaagt cctcggagaa gcccatcggc
     2041 gcgtgatcgg tgtcgatgcc ggccccggcg acaccgccga cggctcgggc cgcgacgtcg
     2101 acctcggccg cggtgttcac cgtcggctcg aaggcccggg cgtagcggag cgtggccgtg
     2161 gccccgtggg cggcagcggt gccctccacg atcgcaccca tccgtgagcg gatcgcttcg
     2221 ctgacgtgct cctcgtagcc gcgcacgtcc ccgcggatcg tcaccgtcgt cgggatgatg
     2281 ttgggggcac cgtcggtgat gagctcggtc accgacacga cgccgtggtc ctggggggcg
     2341 agggcgcggg acacgatgct ctggaggccg atcacgacgt gtgcgccggc cacgagcgga
     2401 tcgatggtgc gctccggcgc cgacgcgtgg ccaccccgcc cgtggatctc gatctcgaag
     2461 cgctcctcgt acgcggtgaa cgggccggac cgggtggcga agcggcccac ggccagcccc
     2521 ggcaggttgt gcatcccgta cacggcgtcc atcgggaagc gctcgaggag cccgtcatcg
     2581 atcatggcct gggccccgcg cccgttctcc tcgtccggct ggaagacgaa gtgcacggtg
     2641 ccgtcgatgt ccgagctgtc cgggccggtg tcggcggcca ggtgcagcgc ggcgccgagc
     2701 agcatcgtgg tgtgaccgtc gtggccgcag ccgtggaacg cacccggcgc ctgcgagcgg
     2761 tgatcgaagg tgttggcctc ctggatcggc aaggcgtcga ggtcggcacg cagcccgatg
     2821 ctgcgactcg acgtgccccg ccgaagcgtg gcgaccacgc cgctcccgcc gacgccacgg
     2881 gtgacgtcga gaccggccgc ggccaaccgt tcggccacca gcccggcaga ggcgtccacg
     2941 tcgaacgcgg tctcgggatg ccggtggagg tgccggcgca gcgaggcgag ctcagcccgg
     3001 ccgggcgcgg tcacgacgca ccgtcctggc gggggagccc ccggccgcga tggggttcga
     3061 cgtgcgggac cacgccccga ggctaggacg ggcgcgccga tgtgccgccg agacgcaggt
     3121 cccgccggca cacccgctca gttgtcggtg cccctggctc ctggggccat cggctcgtac
     3181 cacttccagt cggcccgctc gccggtgggt ccggcgtacg gcggagcctg agggggcggg
     3241 cccgtcggcg gtctggccac cgacgagccg tcgagcgtcc gtccgttcac gtcggtgacg
     3301 gtgaccgcgt ggcccgggcc ggagatggtg atgagcttcc ggtggtggag ccggtggtgg
     3361 aacgggcaga ccagcaccaa gttccagagc tcggtcgggc cgcccttctc ccagtgccag
     3421 atgtggtggg cgtgcaggcc ccgggtggcg ccgcacccgg gcacagcgca cgtcgactgg
     3481 cggtgctcga gcgcccgacg gatgcgacgt gggatctccc gtgtctcgcg gccgacgccg
     3541 atcggcttgc cgtcccgctc gaaccaggtc tccacccggg cgtcgcacgt caggtagagc
     3601 cgctcggcct cggtgagggc cggacccagg tggagcgacc cgatctggga ctccacgtcg
     3661 aggtggacga cg
//
LOCUS       DATISU010000314        17119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_22386, whole genome shotgun sequence.
ACCESSION   DATISU010000314 DATISU010000000
VERSION     DATISU010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 17119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17119
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22386"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VLR27_12365"
     CDS             complement(<1..632)
                     /locus_tag="VLR27_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04294.1"
                     /translation="MGSSPRSSRWKRLLRRTYAVALVVAAVVVLATRRDDVVALVDGA
                     RPGPLLVALALSIVSLGQSAWFWSRALTDLRSPLPYAKVLEATVAAIPGRYLPGSVWY
                     AAGRVGALRGAGASTTALAVVAVLETLLSFVVAVALGAGLLVAGGSDDSGLGVVSLIA
                     VAVALALVSSPWVINPVVRWAAARRGLREVPEVGWSGYAELVGHLVLFWLA"
     gene            complement(638..2545)
                     /locus_tag="VLR27_12370"
     CDS             complement(638..2545)
                     /locus_tag="VLR27_12370"
                     /inference="COORDINATES: protein motif:HMM:NF020075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HSP04295.1"
                     /translation="MPRTRSARGVVRVAGAAALLATATLAAAIAAPPAGAAPTQQDEI
                     SETITVTGRGWGHGRGLGQWGALGYATGRSGGPWDYQTILGHYYGETTPASIANPLGA
                     VTLLEQREQPLLVQRARGVAIEGKEGTTGAVRAVMRPDGDYDVSTATGCAVGNWSEPE
                     VLPSPVRLRAPGTTGEADDVLQVCHDDGTTTGYRGELAAMSRTFDGQDVGIAQTVNLV
                     RLDDLLRSVVPGLLPPAWADLDDGRGRHALLAQAVASRGFLGYGDGRWHDLHSGLGAT
                     FSTCDTARCARYAGVGVEDPRTDDAVRSTSGEVRVRDGLVVRTDYHASSGGWTAGGTF
                     PAVPDVGDALAANKDRTWNTYLDRREIESRYQLGQLQAIQILERNGLGDDGGRVLAMR
                     LVGTAVTVELSGTEFRDDWGLRSDWFQATGVPPRPPVEPREIGDACPEPDVPRDAFTD
                     VDPASVHALAVDCVTWWDVAQGRTATTFAPSATVTRGQMASFVHRMVLAADGNLPEDP
                     TDRFEDDDGTEHEDAINALAAVDVVRGKTATAYAPQEPVERAQAASLVARALLHLDVA
                     TGDVGDAFADDEGLVHESAANTLAAEGILTGVGPGRLAPFAPTQRDQMASLLARTLDL
                     LVDRGIAAVGG"
     gene            complement(2552..3091)
                     /locus_tag="VLR27_12375"
     CDS             complement(2552..3091)
                     /locus_tag="VLR27_12375"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP04296.1"
                     /translation="MGRFLRTLDDLMPDRAPSRVLEVGVGEGEISARVRARYPSASIV
                     GIDLYDPSLSAEWQARGHVGAFADVTRLPFPDDAFDLVLGIEVLEHVADPARALAEVR
                     RVGRDRFVLSVPREPIWRLANLVRGRYARELGNTPGHVNHWSTRAFRALVGDFLEVET
                     TRTPLPWTVVSARHRPARS"
     gene            3275..4249
                     /locus_tag="VLR27_12380"
     CDS             3275..4249
                     /locus_tag="VLR27_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSP04297.1"
                     /translation="MSKLIIQIPCLNEAEQLPVSLAALPREVAGFDEVEWLVIDDGST
                     DDTISVARANGVHHVVKLTNNKGLAYAFEAGIDACLKLGADVIVNTDADNQYDAADIP
                     ALVAPILAGTADMVVGDREVGTIQHFSGTKKALQRLGSAVVRRASGTTVPDATSGFRA
                     YNREAALNLTVVSTFTYTLETLIQAGKSLVAVEHVPVGTNDKLRESRLFGSMSTYVRR
                     NGLALFRIYTSYEPLRVFTTVAVLLWVAALAAWSPFLWDWIVNGRRSGHLQSVILGGV
                     LFMAAVQMFALGVLADVIASHRVVSQRTLERVRRIELQLGVAPSHYDG"
     gene            complement(4237..7686)
                     /locus_tag="VLR27_12385"
     CDS             complement(4237..7686)
                     /locus_tag="VLR27_12385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSP04298.1"
                     /translation="MSPLRLLIIVQRYGKDIRGGAEHAARAVAERLAARGHLVEVLTT
                     TAESYVDWSGDLAPGTSSINGVTVHRLTVDPERDTDVFGRLHHRLIDAEQPADPALEH
                     AWIDAQGPSVPDLEPWLEEHAARFDVAVFFTYLYATTSVGLPVAARHTATLLVPCAHD
                     ELPLQLRAFDRTAQLADAFAFLTPEEARLVHERFRLRSPEHVVGLGTDLAAEADEAAV
                     KRFRAERGIDDRPYLLYVGRIDPSKGTSWLVDRFIELEGELPADLQLVLVGEPVTPVT
                     DHPRVHVIADAADEERDAAMAGAVALVHPSPYESFGMVVTEAWAQRTPVLALGGNDVL
                     RGHIERSGGGLLFTDRATLGAAATVLTEDGEARDALAEAGQAHVRAEHDWDRVIDRWE
                     HALARTADAPRGRPPVLAPARPLPPTRADEPHRLRERGRVEVRDDLPPAWLARPLALV
                     LGLLAGFCIAGVAAAMAGEFRPAVVVPATLVLGGPIGWRSARALPRWRSTRWTHVTAV
                     VMLALVAAITLLNLDDHSQHVVADRDPGVYVTTAKHLADEGNLLLPGPSGPFVDAPNV
                     SANGAGFSPERDDTTLEAQFPHLTAVVLAAAGWVDDAGIFLATPVVGGFALVCLYAFA
                     TLLAGPRWAAVATAVTGIAMPVVIFSRDSYSEPIATALLFGGLWVLSLAERSPRWLPW
                     LLAGLLLGATAMARIDGYLNLAPIALALALHLRLSAGTGRRRLVAVALVVVGVGVAAT
                     LGLADTALFTGGYFDHSLAPRLPAMVAAAVVAGVLGLVLGPRLWQRAPTPDDPARRHP
                     TPLLRAGLWAGAAGLVGFVSWGYWIRPDPEGLPEVALEGMRVLDYLPQAKSLSALWLS
                     WYVGPLGLAAGVLGLAVALVALSKSKRPPIAAVAGLAAVLVTTLLYLWTPSVTPDHPW
                     AMRRFAAVAIPGLAVGVAVLARWLWSFAAPRVGGATVWSSARRLGAGALAVVVAAGSV
                     AGTAAATWPVRDVRAQVPMRDRVNQICELAGDDSAILVPIDGILALMFSVPVGVWCDV
                     PSAGGRPGLGPVDVARLAVAWEQEGRRLVVVSSSETPVMNRLLPAGFVVESIETDTVY
                     PRAVEPTIRRKPERVVADTRLGKGPDGEVTFYVYPTDADAARRYLYETRQQPSRTGER
                     YPS"
     gene            complement(7683..8657)
                     /locus_tag="VLR27_12390"
     CDS             complement(7683..8657)
                     /locus_tag="VLR27_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04299.1"
                     /translation="MNGTRRAVTHVVGRDRPGDPVAAAARALAKTLERCGRPGRVVTA
                     ADVAAGTEDLDGQLVVHSSDGGEALGDALPELVDRTLTLVHHGSAAGADRSVLRALRA
                     PTTSAVALDPVAREELRGMGFRAVAPLQPLLLDDPFDDVDPDEEAAAHLRAHPGPAIL
                     HVGPLVPNRSLEVLLDAFTELVTRHTPGATLSLCGPSPSWYLARLRRRISAHGLRSCE
                     LLEPSDEGQVLARLERADVFVALPPAGLDPYVRRAARDGVPIVAPPGAAATGLDPDDL
                     VELGPRAHAGELAQALEDAIGRGRRSPRVTAPVADGNLDTLVHQLGLR"
     gene            complement(8654..9826)
                     /locus_tag="VLR27_12395"
     CDS             complement(8654..9826)
                     /locus_tag="VLR27_12395"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP04300.1"
                     /translation="MSEDDADPIGHQERVSRLAADIAARLGAEPQSELTAHLARIAAR
                     QTSDPGPSLDAARQALQESPGVPEEAGESKARAAVADAVAQLADEVAAHRRAIDALLD
                     ALADSFRPATHHHTDLEGELDALHDRFAMAERNRAWTPSDLEVIRRLDALEANQRAEG
                     FQPFFSSDRFTDAFRGSQEALEERYQDLADAIAAAATTAGGPVLDLGCGGGDMLALLA
                     ARNVDARGVDSDAESVARAVSRGLRVERGDAVEALRSAADGSLGAVVLIQVVEHLGAQ
                     TLVDVVALAAAKLAPGGLLVAETVNPGSLYVYGHALYLDPTHTTPIHPAYLRFVCEEA
                     GFSSVDVQMRSHPPDEEALPKVNGADEPLVTDVNRVIERLNGILFGPQDYAVLATR"
     gene            9906..10340
                     /locus_tag="VLR27_12400"
     CDS             9906..10340
                     /locus_tag="VLR27_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04301.1"
                     /translation="MTPPDAASSEHHQVLRDAVTGRMAADREAADFPAEQLDAIGVAL
                     ARRTARSPGANVGGRLRELERAADIDVEPPVASERRAGRGVKVAVAKLTNWHVGYLAT
                     QVRDLGNASVRVVRALASRVDELERRVEELERLEQADAEVPS"
     gene            10337..11419
                     /locus_tag="VLR27_12405"
     CDS             10337..11419
                     /locus_tag="VLR27_12405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSP04302.1"
                     /translation="MTTVHQIIPVLAPHDAVGNHTLMVRDALREAGFTSEVFSELRFG
                     DRAGIGLALSDYDAQAGDADLLLYQSSTGSSAAQWLLGRQEPLAVNYHNITPASFFEP
                     WDDEAAASMRRARSQLAALARRSRLALADSPFNAAELVDLGFTPVQVSPLLLDLDARL
                     TAADAPLAEHLARRRDGRSWIFVGRLAPNKCQHDVIAAFAAYRLLYDPGARLTLVGSE
                     AASSYADALLGLVDDLGLAGAVSFTGPVTDAELAAYYDDADVMVCLSRHEGFCVPLLE
                     AMRHDVPVVALAAGAVPDTVGPAGVLVEEADPHLVAATVRELLEDEAARSALVAAGRD
                     VVAAHSLESAKATFVQAIRDHLDARG"
     gene            11412..12623
                     /locus_tag="VLR27_12410"
     CDS             11412..12623
                     /locus_tag="VLR27_12410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSP04303.1"
                     /translation="MAEPSRAGTIAFVPPRFGPDVVGGSEAVMREAAVGLAQRGWDVE
                     VLTTRARDHYTWADAYPAGRDDVDGVRVLRFDMCREEGRYPGQPDRGTLERRIAEGIP
                     LDEGEELAWLNGLFRVPELYHHLLAHADRYRAIVFSPYLFWTTVVGTTVAPERSIAMP
                     CLHDEGYARLRIIARSLSQAARAWYLSGPEQDLARELGLAPAEGELTGAGVPVPETYD
                     ADAFRARHGIERPFLLYAGRREEGKGWPVLVEAWAAAVARGADLDLVTMGVGEIDAPA
                     ELADRVHDLGFLPDAEAPDAFAAAAAYVQPSRNESFSRTIMEAWLAGTPVLANAESAV
                     VAWHCERSGAGVTWTGVDELSAAIEAVAASPGAFRDLAAPGRAYVLEHYTWPTVLDQM
                     ERSLEQMPWPR"
     gene            12620..13270
                     /locus_tag="VLR27_12415"
     CDS             12620..13270
                     /locus_tag="VLR27_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04304.1"
                     /translation="MTRWLVVGPYPPERGTGAEAARTFVEERLRAGDHVHVVSPRPTA
                     AHEHASLDSFGGLKLLRDIAIAQRADGIWLRIEPGIVLRPGTDRRRGLAERAALALLL
                     RRFRERIFDVGDVGLLPGGRAGRPVLQAATRFVTHRAADADALVLHGADPARVHRAGD
                     AEPAPATESTAPVVTEPVAYPPADVVRDLPAERQAIEAAVRARAEQLAAARDAARQ"
     gene            complement(13296..14501)
                     /locus_tag="VLR27_12420"
     CDS             complement(13296..14501)
                     /locus_tag="VLR27_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP04305.1"
                     /translation="MSDTAISIDGVSKRFRINQDRQSSLKERAIGFGRPKRGYKDFWA
                     LRDIDLDIREGETVGLLGHNGSGKSTLLKCVGGILAPTMGEIRVRGRIASLLELGAGF
                     HPDLTGRENVFLNASILGMSRSDIAARFDDIVAFAELEQFIDEQVKHYSSGMYVRLGF
                     AVAINVEPDVLLVDEVLSVGDEAFQQKCLERVRGFQRDGRTIIFVSHAADLVRRICDR
                     VMVLDHGESVAIDDPASAILIFREHIHGGLSLDEDQVLNHGHVVTNQVRIEEVRASWP
                     GSEDHGTIAPGQPLSIAVRYECDEPIDDIVFAMEMRNIDDINVYSCNTDVLEQEVERV
                     EGGGWVTFEIPALDLVDGQYLVDVGCHTHDVITEYDFVHEAARITVVGSGSVAGVANL
                     GAKVTHTPS"
     gene            complement(14504..15400)
                     /locus_tag="VLR27_12425"
     CDS             complement(14504..15400)
                     /locus_tag="VLR27_12425"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP04306.1"
                     /translation="MAADPPILVIAPDRSVLDRIRALWRYRELLVGLVRKELKVKYKD
                     SSLGFMWSLLNPAMYLAVFYVVFQIILGSGIPFFAVYLLAGLLAWNLFNGSVTGATGS
                     IVGNGSLVNKVYFPREILPLAVVGANLVHFFLQTMVLLAGLAIMRYDVALEYLWLTIP
                     ALAVLLVLASALAILFSALNVYARDLQHLLELVMLAWFWMTPIVYSWSLPAEQLSERG
                     LPTGVTLLNPIVPIVIALQRGIWGDDGFVNPDGVLVQVLPDGGPLWYLRNLAVVGVIS
                     LALLVFAMKVFGRLEANFAEEL"
     gene            15483..16784
                     /locus_tag="VLR27_12430"
     CDS             15483..16784
                     /locus_tag="VLR27_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04307.1"
                     /translation="MVTAQLVDRVSNVLARHSNRRGFLSRAAVVGSALTVAGPTYVLR
                     PTSAYAAVCSCLGRSCDCGSLCCDGYTEFCCAIYGENSCPPNTVLAGWWKVDNSHFCN
                     GSARYYMDCNKQSPNCGCGSTGVCRGSDTVCQCRSCSNRKDGCTTFRYGNCNNHISCV
                     GPIMCRVVTCAKPWELDPGCSTVPRTDNNTRYHHRACLDGPPAPPEPRTAPADIMSFQ
                     DGVWRFRRTQSEGPPEVEFSYGQAGDLPLMGDWNGDGTVGIGVRRGRTFILRNEASAG
                     APDHIFDWGRADDIPLVGDWNGNGRDTVGLKRGRTWFLKNRLDGSPHEIQFDWGRDTD
                     LPITGDWSGNGRTGVGIKRGRPWYLKNRLEATTHEIEFAWGRDTDVPVTGDWDGDGRT
                     GVGIVLDYRWYLKNELDPSGHHSEFSFGQPGDTYFGWDQTS"
     gene            complement(16798..>17119)
                     /locus_tag="VLR27_12435"
     CDS             complement(16798..>17119)
                     /locus_tag="VLR27_12435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSP04308.1"
                     /translation="AEADVFVCASEHEGFCIPVLEAMASGLPVVAYASTALPETVGSG
                     GILLPRKDPATVSAAVARLGADDALRTAMVERGLARVQDFDRARTAPRLLAALRSALD
                     LAGG"
BASE COUNT         2347 a         6203 c         6417 g         2152 t
ORIGIN      
        1 gccagccaga acaggaccag gtgcccgacc agctcggcgt agcccgacca gcccacctcg
       61 ggcacctcgc gcagcccacg gcgggccgcc gcccagcgca cgaccgggtt gatcacccac
      121 ggtgagctga ccagggccag cgccacggcg acggcgatga gcgacacgac cccgaggccg
      181 ctgtcgtcgc tgccgccggc cacgagcagg ccggcgccga gcgccacggc gaccacgaag
      241 gacagcagcg tctcgagcac ggccacgacg gcgagcgccg tggtgctggc gccggcaccc
      301 cggagggccc cgacccgtcc ggcggcgtac cagacgctgc cgggcaggta gcggccgggg
      361 atggcggcga cggtggcctc gagcaccttg gcgtagggga gcgggctgcg caggtcggtg
      421 agcgcacggc tccagaacca cgcgctctgg ccgagcgaca cgatggacag ggccagcgcg
      481 accagcagcg ggccggggcg tgcgccgtcc acgagcgcca ccacgtcgtc gcggcgggtg
      541 gccagcacga ccacggccgc caccacgagc gcgacggcat aggtgcggcg caggagccgc
      601 ttccaccgcg acgagcgcgg cgaagatccc acggtggcta cccgcccaca gcagcgatgc
      661 cgcgatcgac gagcaggtcg agggtgcgag ccaggaggct ggccatctgg tcgcgctggg
      721 tcggtgcgaa gggggcgagg cgacccggcc ccacgccggt gaggatgccc tcggcggcca
      781 gcgtgttcgc cgccgactcg tgcaccagcc cctcgtcgtc ggcgaacgcg tcgcccacat
      841 cgccggtggc gacgtccagg tgcagcagcg cccgggcgac gagcgaggcg gcctgcgcac
      901 gctccaccgg ctcctggggt gcgtacgcgg tcgcggtctt gccacgcacg acgtcgacgg
      961 cggccagcgc gttgatcgcg tcctcgtgct cggtgccgtc gtcgtcctcg aaccggtcgg
     1021 tggggtcctc cgggaggttc ccgtcggcgg ccagcaccat gcggtgcacg aacgaggcca
     1081 tctgcccccg ggtcacggtg gcgctcggcg cgaacgtggt ggcggtccgg ccctgggcga
     1141 cgtcccacca ggtcacgcag tcgacggcca gcgcgtggac gctggccggg tcgacgtcgg
     1201 tgaaggcgtc gcggggcacg tccggctccg ggcaggcatc gccgatctcg cggggctcga
     1261 ccggcgggcg cgggggcacc ccggtggcct ggaaccagtc ggagcgcagg ccccagtcat
     1321 cacggaactc ggtgccgctg agctccacgg tgacggccgt gccgacgagg cgcatggcga
     1381 ggacccgccc accgtcgtcg ccgagcccgt tgcgctcgag gatctggatc gcctgcagct
     1441 ggccgagctg gtacctggac tcgatctcgc ggcggtcgag gtacgtgttc caggtccggt
     1501 ccttgttggc ggcgagcgcg tcgccgacat cgggcacggc ggggaacgtg ccgccggccg
     1561 tccacccacc gctggaggcg tggtagtcgg tacgcacgac gaggccgtcg cgcacccgca
     1621 cctccccgga ggtcgagcgc acggcgtcgt cggtgcgggg gtcctcgacg cccacgccgg
     1681 cgtagcgggc gcagcgcgcc gtgtcgcagg tgctgaaggt ggcgccgagc ccgctgtgga
     1741 ggtcgtgcca gcgcccgtcg ccgtagccga ggaacccacg agaggccacg gcctgggcga
     1801 gcagtgcgtg gcgtccccgc ccgtcgtcga ggtcggccca cgccggcggg agcaggccgg
     1861 ggacgacgct gcgcagcagg tcgtcgaggc gcaccaggtt gacggtctgg gcgatgccga
     1921 cgtcctggcc gtcgaaggtg cgggacatgg cggccagctc gccccggtag ccggtggtgg
     1981 tgccgtcgtc gtggcacacc tgcagcacgt cgtcggcctc gcccgtggtg ccgggcgccc
     2041 gcaggcggac cgggctcggg agcacctccg gctcggacca gttgccgacg gcgcagccgg
     2101 tggcggtgga gacgtcgtag tcaccatcgg ggcgcatcac cgcccggacc gcgccggtgg
     2161 tgccttcctt gccctcgatg gcgacgcccc gggcccgctg gacgagcagg ggctgctcgc
     2221 gctgctcgag gagggtgacg gcacccaggg ggttggcgat cgaggccggt gtggtctcgc
     2281 cgtagtagtg gccgaggatc gtctggtagt cccacgggcc gccggagcgc ccggtggcgt
     2341 acccgagggc gccccactgt cccaagcccc ggccgtgacc ccacccgcgt ccggtgacgg
     2401 tgatggtctc ggagatctcg tcctgctggg tcggcgcggc gccggcgggc ggagcggcga
     2461 tggctgcggc gagggtggcg gtggcgagca gcgcggcggc gccggcgacc cgcacgacgc
     2521 cgcgagccga acgggtgcgt ggcatgcgtc ctcagctccg ggctggtcgg tgacgggccg
     2581 acactaccgt ccacggcagc ggggtcctcg tggtctcgac ctcgaggaag tcgccgacga
     2641 gcgcacggaa cgcgcgggtc gaccagtggt tgacgtggcc tggcgtgttc ccgagctccc
     2701 gggcgtagcg accccggacg aggttggcca ggcgccagat cggctcgcgg ggcacggaca
     2761 gcacgaagcg gtcccgcccg acccgtcgca cctcggcgag cgcccgggcc gggtcggcga
     2821 cgtgctcgag cacctcgatg cccaggacca ggtcgaacgc gtcgtccggg aacgggaggc
     2881 gggtgacgtc ggcgaaggcc ccgacgtggc cccgggcctg ccactcggcg ctcagcgacg
     2941 ggtcgtacag gtcgatgccg acgatggacg ccgacgggta ccgggcccgc acccgggccg
     3001 agatctcgcc ttccccgacg cccacctcga gcacccggct gggcgcgcgg tccggcatca
     3061 gatcgtcgag cgtgcgcagg aaccgaccca ccagccgctg ctcgagtgga tgcccggtcg
     3121 cgtacttgtc gtagctgttc cccgtcggcc cggaggacgt tggggagacg tctgcagagg
     3181 tcaacgggtc tccgtcggca ccgatcgggg gtgtgtgcgg gctgcgccag cctagtggac
     3241 gctccgtggt ggtgccctag ggtctgcgct cgtcatgtcg aagctgatca tccagatccc
     3301 ctgcctcaac gaggccgagc agctgccggt ctcgctcgcc gcgctcccgc gcgaggtggc
     3361 gggcttcgac gaggtcgagt ggctggtgat cgacgacggc tccaccgacg acaccatctc
     3421 ggtggcccgg gccaacggcg tgcaccacgt cgtcaagctc accaacaaca aggggctggc
     3481 gtacgcgttc gaagccggga tcgatgcctg cctgaagctg ggcgctgacg tgatcgtcaa
     3541 caccgacgcc gacaaccagt acgacgccgc cgacatcccc gccctcgtcg cgccgatcct
     3601 ggccgggacg gcggacatgg tggtgggcga ccgcgaggtc ggcaccatcc agcacttctc
     3661 gggcaccaag aaggcgctgc agcgcctcgg cagcgcggtg gtgcgacggg cgtcgggcac
     3721 cacggtgccg gacgcgacct ccggcttccg ggcctacaac cgcgaggcgg cgctgaacct
     3781 cacggtggtg tccacgttca cctacacgtt ggagaccctc atccaggccg gcaagagcct
     3841 ggtggcggtc gagcacgtgc cggtcggcac caacgacaag ctgcgcgagt cccggctgtt
     3901 cggctcgatg tccacctacg tgcgacgcaa cgggctggct ctgttccgca tctacacctc
     3961 ctacgagccg ctgcgcgtgt tcaccaccgt cgcggtgctc ctgtgggtcg cggcgctggc
     4021 ggcgtggagc ccgttcctgt gggactggat cgtgaacggg cgtcggtccg gccacctgca
     4081 gtcggtgatc ctcggcgggg tgctgttcat ggccgccgtg cagatgttcg ccctcggcgt
     4141 gctcgcggac gtgatcgcgt cacaccgcgt cgtgagccag cggacgcttg agcgggtgcg
     4201 ccgcatcgag ctgcagctgg gcgtggcgcc ctcgcactac gacgggtagc gctcgcccgt
     4261 cctcgacggc tgctgccgcg tctcgtacag gtaccggcgg gccgcgtcgg cgtcggtcgg
     4321 gtacacgtag aacgtcacct cgccgtccgg gcccttgccg agccgcgtgt cggccacgac
     4381 gcgctcgggc ttccgccgga tcgtgggctc gacggcacgc gggtagacgg tgtcggtctc
     4441 gatcgactcg accacgaagc cggcggggag cagccggttc atgacgggcg tctccgacga
     4501 cgagaccacc acgaggcgcc ggccctcctg ctcccacgcc accgccaggc gggcgacgtc
     4561 gacggggccg agcccgggtc ggccgccggc gctgggcacg tcgcaccaga caccgacggg
     4621 caccgagaac atcagggcga ggatgccgtc gatgggcacg aggatggcgg agtcgtcccc
     4681 ggccagctcg cagatctggt tgacgcggtc gcgcatcggg acctgggcgc gcacgtcccg
     4741 caccggccac gttgcggcgg ccgtgccggc caccgagccg gcggcgacca ccacggccag
     4801 cgcaccggcc ccgagtcggc gggccgagga ccacaccgtc gcgcccccga cgcgcggcgc
     4861 ggcgaaggac cagagccagc gggcgagcac cgccacaccc acggccaggc cggggatggc
     4921 gaccgcggcg aagcgccgca tcgcccacgg gtggtccggc gtgacgctgg gggtccacag
     4981 gtacagcagc gtggtgacca gcacggcggc cagccccgcc accgcagcga tgggcggccg
     5041 tttgctcttg ctgagcgcga ccagggccac ggcgagaccg agcacgccgg cggccagccc
     5101 cagcgggccg acgtaccagg acagccacag ggccgacagc gacttggcct gcgggaggta
     5161 gtccagcacc cgcatgccct cgagcgcgac ctccggcagc ccctcggggt ccgggcggat
     5221 ccagtagccc caggagacga acccgaccag accggcggcg ccggcccaca gcccggcccg
     5281 caggagcggc gtgggatgcc ggcgcgccgg gtcgtccgga gtgggcgcgc gctgccacag
     5341 ccgggggccg agcaccaggc cgagcacccc cgcgaccacc gcggcggcga ccatcgcggg
     5401 caggcgaggg gcgaggctgt ggtcgaagta cccgccggtg aacagcgccg tgtcggcgag
     5461 gccgagcgtc gccgcgaccc cgaccccgac cacgaccagg gccacggcca ccagccgacg
     5521 ccggcccgtg ccggcgctca ggcgcaggtg cagggccaac gcgagcgcga tgggtgcgag
     5581 gttgaggtag ccgtcgatgc gggccatcgc ggtggcgccg aggagcagac cggcgagcag
     5641 ccagggcagc cagcgtggcg accgctcggc gaggctcagc acccacaggc cgccgaacag
     5701 cagggccgtg gcgatcggct cggagtagga gtcgcgggag aagatcacca cgggcatggc
     5761 gatgccggtc acggccgtgg ccaccgccgc ccaccggggt ccggcgagca gggtggcgaa
     5821 cgcgtacagg cacacgaggg cgaagccgcc gaccacgggc gtggccagga agatgccggc
     5881 gtcgtccacc cagccagcgg cagccagcac gacggcggtg aggtggggga actgggcctc
     5941 gagggtggtg tcgtcccgct cgggcgagaa ccccgcgccg ttggcggaca cgttcggagc
     6001 gtcgacgaag ggcccgctcg ggccggggag gagcaggttg ccctcgtcgg ccaggtgctt
     6061 cgccgtggtg acgtagacac ccgggtcgcg gtcggccacg acgtgctggc tgtggtcgtc
     6121 caggttgagc agcgtgatcg ccgccaccag cgccagcatg accacggcgg tcacgtgggt
     6181 ccaccgggtg ctccgccagc gggggagcgc gcgggcgctc cgccacccga tcgggccgcc
     6241 cagcaccagg gtggccggca ccacgacggc ggggcggaac tccccggcca tcgccgccgc
     6301 cacgccggcg atgcagaacc cggcgagcag gccgagcacc agcgccaggg gtcgggccag
     6361 ccacgccggg gggaggtcgt ctcgcacctc gacccggccg cgctcgcgca ggcggtgggg
     6421 ttcgtcggct cgcgtcgggg gcagcggtcg agccggggcc aggacgggcg gccggccccg
     6481 cggtgcgtcg gccgtccggg ccagggcgtg ctcccaccgg tcgatgacgc ggtcccagtc
     6541 gtgctcggcc cgcacgtgcg cctgcccggc ctcggcgagc gcgtcgcgcg cctcaccgtc
     6601 ctcggtgagc acggtcgcgg cggcccccag ggtcgcccgg tcggtgaaca gcaggccgcc
     6661 gccgctgcgc tcgatgtgcc cgcgcaggac gtcgttgccg ccgagggcga gcaccggggt
     6721 ccgctgcgcc cacgcctcgg tgaccaccat gccgaagctc tcgtaggggg acgggtggac
     6781 cagggcgacg gcaccggcca tcgccgcgtc gcgctcctcg tcagcggcgt cggcgatcac
     6841 gtgcacccgg gggtggtcgg tcaccggggt caccggctcg ccgaccagca ccagctggag
     6901 gtcggcgggc agctcgccct ccagctcgat gaaccggtcg accagccacg acgtcccctt
     6961 gctcgggtcg atccggccca cgtacagcag gtaggggcgg tcgtcgatcc cccgctcggc
     7021 gcggaaccgc ttcaccgcgg cctcgtcggc ttcggcggca aggtccgtgc cgaggcccac
     7081 gacgtgttcg ggcgagcgca ggcggaaccg ctcgtggacg agccgggcct cttcgggggt
     7141 gaggaaggcg aaggcgtcgg ccagctgcgc ggtccggtcg aaggcgcgca gctgcagcgg
     7201 cagctcatcg tgcgcgcacg gcaccagcaa cgtggcggtg tggcgcgccg ccaccgggag
     7261 cccgacgctg gtggtggcgt agaggtacgt gaagaacacg gccacgtcga agcgggctgc
     7321 gtgctcctcg agccatggct cgaggtcggg caccgacggg ccctgcgcgt cgatccacgc
     7381 gtgctcgagc gccgggtctg cgggctgctc ggcatcgatg aggcggtggt gcagccggcc
     7441 gaagacgtcg gtgtcgcgct cgggatcgac ggtgagccgg tgcaccgtga ccccgttgat
     7501 ggagctggtg cccggggcca ggtcgccgga ccagtcgacg taggactccg cggtggtggt
     7561 gagcacctcg acgaggtggc cgcgtgccgc gaggcgctct gctacggccc gcgccgcgtg
     7621 ctcggcaccg ccccggatgt ccttgccgta gcgctggacg atgatcagca gccgcaacgg
     7681 gctcatcgca gcccgagctg gtgcaccagc gtgtcgaggt tcccgtcggc cacgggagcc
     7741 gtcacgcgtg gggatcgccg gccccggccg atcgcgtcct cgagggcttg ggcgagctcg
     7801 ccggcgtggg cgcgagggcc gagctcgacg aggtcgtcgg gatcgagtcc ggtggcggcc
     7861 gcgcccggcg gggcgacgat gggcacgccg tcacgggcgg ctcggcgcac gtaggggtcg
     7921 aggccggcag gcgggagggc gacgaacacg tcggcccgct ccagccgggc gagcacctgg
     7981 ccctcgtcgc tcggctcgag gagctcgcac gaccgcaggc cgtgggcgct gatccgccgg
     8041 cggagccggg cgaggtacca cgacggcgac ggaccgcaca gcgagagggt ggcgccgggc
     8101 gtgtgccggg tgaccagctc ggtgaaggcg tcgaggagca cctcgagcga gcggttcggg
     8161 acgagcgggc cgacgtggag gatcgcaggg ccggggtggg cccgcaggtg cgccgccgcc
     8221 tcctcgtcgg ggtcgacgtc gtcgaacggg tcgtcgagca gcagcggctg gagcggcgcc
     8281 accgcgcgga agcccatgcc ccgcagctcc tcgcgcgcca ccgggtccag ggccacggcg
     8341 gaggtggtcg gggcgcgcag cgcacgcagc acgctgcggt cggcaccggc ggcggacccg
     8401 tggtgcacga gcgtcagggt ccggtcgacg agctcgggca gggcgtcgcc gagcgcctcg
     8461 ccgccgtcgc tgctgtggac gaccagctgg ccgtcgaggt cctcggtgcc cgcggcgacg
     8521 tcggccgcgg tcacgacccg gcccgggcgt ccgcagcgct cgagggtctt ggcgagggca
     8581 cgggcggccg ccgccacggg gtcacccggg cggtcccggc ccaccacgtg cgtgaccgcc
     8641 cgccgggtgc cgttcatcgg gtggcgagca ccgcgtagtc ctgcggtccg aacaggatgc
     8701 cgttgaggcg ctcgatgacc cggttgacgt cggtgaccag cggttcgtcg gcgccgttga
     8761 ccttcggcag ggcctcctcg tccggcgggt gcgagcgcat ctggacgtcg acggaggaga
     8821 acccggcctc ctcgcacacg aaccgcaggt aggcggggtg gatcggcgtg gtgtgcgtcg
     8881 ggtccaggta gagggcgtgc ccgtacacgt agagcgagcc cgggttgacc gtctcggcga
     8941 cgagcaggcc gccgggcgcc agcttcgccg cggcgagcgc gaccacgtcc accagcgtct
     9001 gggccccgag gtgctcgacc acctggatga gcaccaccgc gcccagcgag ccgtcggccg
     9061 ccgagcggag cgcctcgacg gcatcgcccc gttccacgcg gagcccgcgc gacacggccc
     9121 gagcgaccga ctcggcgtcg ctgtcgacgc cgcgggcgtc gacgttgcgg gcggcgagca
     9181 gggccagcat gtcgccgccc ccgcacccga ggtccagcac cgggccgccg gcggtggtgg
     9241 cggcagcggc gatggcgtcg gcgaggtcct ggtagcgctc ctcgagcgcc tcctggctgc
     9301 cccggaaggc gtcggtgaac cggtcgctgc tgaagaacgg ctggaagccc tcggcccgct
     9361 ggttcgcctc gagggcgtcg agccggcgga tgacctcgag gtcgctcggc gtccacgccc
     9421 ggttgcgctc ggccatggcg aagcggtcgt gcagcgcgtc gagctcgccc tcgaggtccg
     9481 tgtggtggtg cgtggcgggg cggaaggagt cggccagcgc gtcgagcagg gcgtcgatcg
     9541 cccgacgatg ggcggcgacc tcgtcggcga gctgggcgac ggcgtcggcg accgcggccc
     9601 gggccttgct ctcgcctgcc tcctccggga cgccgggtga ctcctgcagc gcctggcggg
     9661 ccgcgtccag cgacgggccg ggatcgctgg tctgtcgggc ggcgatgcgg gccaggtggg
     9721 cggtcagctc ggactggggc tcggcgccga ggcgggcggc gatgtcggcc gcgagccgcg
     9781 agaccctctc ctggtggccg atcggatcgg cgtcgtcctc ggacacgaac ggcgagccta
     9841 ctgacgtctc ggcgggtgct cggggttcgg ggggcgggcg gaccccgtcg gtagagtcgc
     9901 ccgccgtgac cccaccggac gcagcctcct ccgaacacca ccaggtgctc cgcgacgccg
     9961 tgaccgggcg catggccgcc gatcgggagg ccgccgactt cccggccgag cagctcgacg
    10021 cgatcggggt ggccctggcc cgccgcacgg cgcgcagccc cggcgccaac gtgggcggtc
    10081 ggctgcggga gctggagcgg gccgccgaca tcgacgtcga gccgccggtg gcgtcggagc
    10141 gacgcgccgg ccggggcgtg aaggtcgcgg tcgccaagct caccaactgg cacgtcggct
    10201 acctcgccac ccaggtgcgc gacctcggca acgcctcggt ccgggtcgtg cgcgccctcg
    10261 cctcccgggt cgacgagctc gagcgtcgcg tcgaggagct ggagcggctg gagcaggccg
    10321 acgccgaggt gccgtcgtga ccacggtcca ccagatcatc ccggtgctcg ccccccacga
    10381 cgccgtcggc aaccacaccc tgatggtgcg cgacgcgctg cgggaggccg gcttcacctc
    10441 cgaggtcttc tccgagctcc gcttcggcga ccgggccggc atcggcctgg ccctgagcga
    10501 ctacgacgcc caggccggcg acgccgacct gctgctctac cagagctcca ccgggtcctc
    10561 cgcggcccag tggctgctcg gtcggcagga gccgctggcc gtcaactacc acaacatcac
    10621 gccggcctcg ttcttcgagc cgtgggacga cgaggccgcg gccagcatgc gccgggcccg
    10681 ctcccagctc gctgcgctgg cccgccggtc ccgcctcgcc ctggccgact cgccgttcaa
    10741 cgccgccgag ctcgtcgacc tcgggttcac gccggtgcag gtgtcgcccc tgctgctcga
    10801 cctcgacgcc cgcctcaccg cggccgacgc gccgctggcc gagcacctcg cccgccgccg
    10861 cgacggccgg agctggatct tcgtcggccg gctggcgcca aacaagtgcc agcacgacgt
    10921 gatcgccgcg ttcgccgcct accggctgct gtacgacccg ggcgcgcggc tcacgctggt
    10981 cggcagcgag gcggcgtcgt cctacgccga cgccctgctc ggcctggtcg acgacctcgg
    11041 cctcgccggc gccgtgtcct tcaccggacc ggtcaccgac gccgagctgg ccgcctacta
    11101 cgacgacgcc gacgtcatgg tgtgcctctc ccgccacgag gggttctgcg tgccgctgct
    11161 ggaggccatg cgccacgacg tgccggtcgt cgccctggcg gccggcgccg tgcccgacac
    11221 cgtcggtccg gccggcgtgc tggtcgagga ggccgacccc cacctggtgg ccgccaccgt
    11281 gcgggagctg ctcgaggacg aggccgcccg gagcgcgctg gtcgcggccg gtcgcgacgt
    11341 cgtcgccgcc cactcgttgg agtcggccaa ggccacgttc gtgcaggcga tccgcgacca
    11401 cctcgacgcc cgtggctgag ccgagccggg ccggcaccat cgccttcgtc ccgccccgct
    11461 tcggacccga cgtggtgggc ggctccgaag ccgtgatgcg cgaggccgcc gtcggcctgg
    11521 ctcagcgggg ctgggacgtg gaggtgctca ccacccgtgc ccgcgaccac tacacctggg
    11581 ccgacgccta cccggccggg cgcgacgacg tcgacggcgt gcgggtgctc cgcttcgaca
    11641 tgtgccggga ggagggccgc tacccgggtc agcccgaccg gggcacgctc gagcgccgca
    11701 tcgccgaggg catccccctc gacgagggcg aggagctggc ctggctcaac ggcctgttcc
    11761 gggtgcccga gctgtaccac cacctgctgg cgcacgccga ccggtaccga gccatcgtct
    11821 tctcgccgta cctgttctgg accaccgtcg tgggcaccac cgtggcgccc gagcgcagca
    11881 tcgccatgcc gtgcctgcac gacgagggct acgcccgcct gcggatcatc gcccgctcgc
    11941 tgtcccaggc cgcccgggcc tggtacctgt cgggtccgga gcaggacctg gcccgggagc
    12001 tcggcctggc cccggcggag ggcgagctca ccggcgccgg cgtgcccgtg cccgagacct
    12061 acgacgccga cgcgttccgc gcgcggcacg gcatcgagcg cccgttcctg ctgtacgcgg
    12121 ggcggcgcga ggagggcaag ggctggcccg tgctcgtcga ggcgtgggca gcagcggtcg
    12181 cccggggtgc cgacctcgac ctggtcacga tgggcgtcgg cgagatcgat gcgcccgccg
    12241 agctggccga tcgggtccac gacctcgggt tcctgcccga tgctgaggcg cccgacgcct
    12301 tcgccgccgc agccgcctac gtgcagccca gccgcaacga gagcttctcc cgcacgatca
    12361 tggaggcgtg gctggccggc accccggtgc tggcaaacgc cgagagcgcg gtggtcgcct
    12421 ggcactgcga gcgctccggt gccggcgtga cctggaccgg tgtggacgag ctgtcggccg
    12481 ccatcgaggc ggtggctgcg tcgcccggcg cgttccgtga cctggccgcg cccggccggg
    12541 cctacgtgct cgagcactac acgtggccca cggtgctcga ccagatggag cgcagcttgg
    12601 agcagatgcc gtggccccga tgacccgctg gctggtggtc ggcccctacc cgcccgagcg
    12661 aggcacgggc gcggaggcgg cccgcacctt cgtcgaggag cgcctgcgcg ccggcgacca
    12721 cgtgcacgtg gtctcgcccc gccccaccgc cgcccacgag cacgcctcgc tcgacagctt
    12781 cggcggcctc aagctgctcc gggacatcgc catcgcccag cgcgccgacg gcatctggct
    12841 gcggatcgaa cccggcatcg tgctgcggcc cggcaccgac cggcgccggg ggctcgccga
    12901 gcgggctgcg ctcgcactgc tgctgcgccg gttccgcgag cggatcttcg acgtgggcga
    12961 cgtcggcctg ctgcccggcg gccgggccgg ccggccggtg ctccaggcag cgacgcgatt
    13021 cgtcacccac cgcgccgccg acgccgacgc cctggtcctc cacggcgccg atccggcccg
    13081 cgtgcaccgc gccggcgacg ccgaaccggc acccgcaacg gagagcacag cgccggtcgt
    13141 caccgagccg gtggcgtacc cgccggccga cgtggtgcgc gacctgcccg ccgagcggca
    13201 ggccatcgag gccgcggtcc gggcccgggc cgagcagctc gccgccgccc gcgacgcggc
    13261 ccgccagtag ctcgttccgc agccgctgcc agaactcagc tgggggtgtg cgtgaccttg
    13321 gcgccgaggt tggcgacgcc ggcgacgctg cccgaaccca cgacggtgat gcgagcggcc
    13381 tcgtgcacga agtcgtactc ggtgatgacg tcgtgggtgt ggcagccgac gtcgacgagg
    13441 tactgcccgt cgacgaggtc gagcgccggg atctcgaacg tgacccagcc gccgccctcg
    13501 acccgttcga cctcctgctc gagcacgtcg gtgttgcagc tgtagacgtt gatgtcgtcg
    13561 atgttgcgca tctccatggc gaagacgatg tcgtcgatgg gctcgtcgca ctcgtagcgc
    13621 acggcgatgg agagcggctg gcccggggcg atggtgccgt ggtcctcgga gccgggccag
    13681 gaggcgcgga cctcctcgat gcgcacctgg ttggtgacca cgtggccgtg gttgagcacc
    13741 tggtcctcgt cgagggacag gccgccgtgg atgtgctcgc ggaagatcag gatcgcggag
    13801 gcggggtcgt cgatggccac cgactcgccg tggtcgagca ccatgacccg gtcgcagatg
    13861 cgtcgcacca ggtcggcggc gtgggacacg aagatgatcg tgcgcccgtc gcgctggaag
    13921 ccccgcaccc gctccaggca cttctgctgg aaggcctcgt cgcccaccga gagcacctcg
    13981 tcgaccagca gcacgtcggg ctcgacgttg atggccacgg cgaagccgag ccgcacgtac
    14041 atgcccgagg agtagtgctt gacctgctcg tcgatgaact gctcgagctc ggcgaaggcg
    14101 acgatgtcgt cgaagcgcgc ggcgatgtcg ctgcgggaca tgccgaggat cgacgcgttg
    14161 aggaacacgt tctcccggcc ggtgaggtcc gggtggaacc cggcgcccag ctcgagcagc
    14221 gaggcgatgc ggccccgcac gcggatctcg cccatggtgg gggcgaggat gccgccgacg
    14281 cacttgagca gcgtggactt gcccgagccg ttgtggccga gcaggccgac cgtctcaccc
    14341 tcgcggatgt cgaggtcgat gtcgcgcagc gcccagaagt ccttgtagcc ccgcttgggt
    14401 cgcccgaagc cgatggcgcg ctccttgagc gagctctgcc ggtcctggtt gatccggaag
    14461 cgcttggaga cgccgtcgat ggagatcgcc gtgtcggaca ccgtcagagc tcctcggcga
    14521 agttggcctc gagacgcccg aagaccttca tggcgaacac gagcagcgcc agcgagatga
    14581 cacccaccac cgcgaggttg cgcaggtacc agagcggacc gccgtcgggc agcacctgga
    14641 ccagcacgcc gtcggggttc acgaaaccgt cgtcacccca gatgccccgc tggagggcga
    14701 tgacgatcgg cacgatcggg ttcagcagcg tgacgccggt gggcagcccc cgctcggaca
    14761 gctgctcggc cggcagcgac cacgagtaga cgatgggggt catccagaac caggccagca
    14821 tcacgagctc cagcaggtgc tgcaggtcgc gggcgtacac gttgagggcg ctgaagagga
    14881 tggccagcgc ggatgccagc accagcagca ccgccagggc ggggatggtc agccacaggt
    14941 actcgagcgc cacgtcgtag cgcatgatgg cgaggccggc gagcagcacc atcgtctgca
    15001 ggaagaagtg gacgaggttg gcgcccacca cggccaacgg gaggatctcg cgcgggaagt
    15061 acaccttgtt gaccagcgag ccgttgccga cgatcgagcc ggtggcaccg gtgacgctgc
    15121 cgttgaacag gttccaggcc agcagcccgg cgaggagata cacggcgaag aaggggatgc
    15181 cgctgccgag gatgatctgg aagaccacgt agaacacggc caggtacatc gccgggttca
    15241 gcagcgacca catgaagccg aggctgctgt ccttgtactt gaccttcagc tccttgcgga
    15301 ccaggccgac caggagctcc cggtagcgcc acagggcccg gatgcggtcg aggacggacc
    15361 gatcgggcgc gatgaccagg atgggggggt cagcagccat gagctgggca atcgtaccgg
    15421 cccggtgggc gggggtcgcc aaccgccggg cccgcccacc gccttcgcta gggtcgtcgc
    15481 ccgtggtcac cgcacagctc gtcgaccggg tcagcaacgt cttggcccgc cactcgaacc
    15541 gtcgtgggtt cctctccagg gccgccgtcg tcggcagcgc cctcaccgtg gccgggccga
    15601 cctacgtgct ccgtcccacc agcgcgtacg ccgccgtgtg ctcctgcctc ggccgcagct
    15661 gcgactgcgg cagcctgtgc tgcgacgggt acaccgagtt ctgctgcgcc atctacggcg
    15721 agaactcctg cccgccgaac acggtcctcg ccgggtggtg gaaggtcgac aactcccact
    15781 tctgcaacgg atcggcccgc tactacatgg actgcaacaa gcagtccccg aactgcgggt
    15841 gcggctccac cggcgtgtgc cggggcagtg acacggtgtg ccagtgccgc agctgcagca
    15901 accgcaagga cggctgcacc accttccgct acggcaactg caacaaccac atctcctgcg
    15961 tcgggccgat catgtgccgg gtggtcacct gcgccaagcc ctgggagctc gacccgggct
    16021 gcagcaccgt cccccgcacc gacaacaaca cccgctacca ccaccgcgcc tgcctcgacg
    16081 gcccgccggc gccgcccgaa ccccgcaccg cgccggccga catcatgtcg ttccaggacg
    16141 gcgtgtggcg gttccggcgc acccagtccg agggtccgcc ggaggtcgag ttctcctacg
    16201 gccaggccgg cgacctgccc ctcatggggg actggaacgg cgacggcacg gtcggcatcg
    16261 gcgtgcggcg gggccgcacg ttcatcctcc gcaacgaggc gtccgccggc gcccccgacc
    16321 acatcttcga ctggggccgg gccgacgaca tcccgctggt cggcgactgg aacggcaacg
    16381 ggcgcgacac ggtcggcctg aagcggggcc ggacgtggtt cctgaagaac cgcctcgacg
    16441 gcagcccgca cgagatccag ttcgactggg gccgcgacac cgacctgccg atcaccggcg
    16501 actggagcgg caacggccgc accggcgtcg gcatcaagcg gggccgcccg tggtacctca
    16561 agaaccgcct cgaagcgacc acgcacgaga tcgagttcgc gtggggccgg gacaccgacg
    16621 tgccggtcac cggcgactgg gacggcgacg gccgcaccgg cgtcggcatc gtgctcgact
    16681 accgctggta cctcaagaac gagctcgacc cgagcggcca ccactccgag ttcagcttcg
    16741 gtcagcccgg cgacacctac ttcggctggg accagaccag ctgacgtccg ccggcgttca
    16801 gccgccggcg aggtccagcg cgctgcggag cgcggccagc aggcggggcg cggtccgggc
    16861 ccggtcgaag tcctgcaccc gggcgaggcc gcgctcgacc atcgccgtcc gcagcgcgtc
    16921 gtcggcgccg aggcgggcca cggcggccga caccgtggcc gggtccttgc ggggcaggag
    16981 gatgccgccg ctcccgaccg tctcgggcag ggcggtggac gcgtaggcca ccaccgggag
    17041 gccgctggcc atcgcctcga gcaccgggat gcagaagccc tcgtgctcgg aggcgcacac
    17101 gaacacgtcg gcttcggcg
//
LOCUS       DATISU010000315         3757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_488253, whole genome shotgun sequence.
ACCESSION   DATISU010000315 DATISU010000000
VERSION     DATISU010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488253"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VLR27_12440"
     CDS             complement(<1..1017)
                     /locus_tag="VLR27_12440"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HSP04309.1"
                     /translation="MTDATPAFDVVLFGPTGVTGREVARHLHRRAPELGLTWAVAGRD
                     RDRIERSLASVGAEPADILHADTDDRASIEAMVGAAKVVADLVGPFVRHGEPVYAACA
                     RAGTHCIDPCGEIDWVLDMIRRYDDRATESGSIIVPTAGFEALPFDLGALLAAHLLHE
                     RTGLGVASVDVAVTITSERDLRVRSAADAVSGGTFASGLEQIRRGRPEVSDPHVLDTS
                     GDHSGRYDLRPRKHSGTGRWLSPMVPSPFLNPPVVHRSASLLRDSGDTIHASDFEYRE
                     GVDTAGMLPGLLAAPAAVAGSLLAVGMGTLTGAREKIRRSFVDVLERVGPSPGDGPRP
                     EDLDR"
     gene            1074..1784
                     /locus_tag="VLR27_12445"
     CDS             1074..1784
                     /locus_tag="VLR27_12445"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HSP04310.1"
                     /translation="MCGRFVSTSPPDELAAYFGAEAVAESVLEPNYNVAPTQDVLAVV
                     EADGGRFLDAFFWGLVPSWAKDVKIGSRMINARAETLAEKSAFKKPFAARRCLVPADG
                     FYEWRKLDSSKKPKKQPMFIHRSDGEPLAFAGIWSVWRGPDKDQEPLRSLSIVTTSPN
                     ELMATIHDRMPVILPEERWATWLDRDNHDLDSLTDLLVPLPDGLLTATPVSTLVNNVR
                     NNGPELLEPEAVEQTTLL"
     gene            complement(1804..2223)
                     /locus_tag="VLR27_12450"
     CDS             complement(1804..2223)
                     /locus_tag="VLR27_12450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HSP04311.1"
                     /translation="MATVFTKIIEGELPGRFVWRDDRCVAFLSINPLRHGHALVVPIV
                     EVDHWLDLDPELNSHLMEVSQRIGQAQMAAFSPTRVGLMIAGLEVPHVHLHVVPIDGV
                     HDMDFANAARDPDAAAMDQARDDLVAALRDAGHDPGV"
     gene            2260..2925
                     /locus_tag="VLR27_12455"
     CDS             2260..2925
                     /locus_tag="VLR27_12455"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSP04312.1"
                     /translation="MSEPVEDAAESAVAPDDAIILDEDVVEDAGLLTIPNLITVVRLC
                     TLPVFVYLLFGADDRWAAGWVLFLIGSTDWLDGYLARRLGQVSNVGKILDPVADRLLF
                     FVGIGSIIIDGSVPLWFAVAVLVREAAVAIGTLSLAALGATRIDVTWYGKAATFALMG
                     AFPSFLVGASDHATADGFRVLAWVVGIPGLALAYWSALLYIPLGRKALAEGRQARLDR
                     TSA"
     gene            2987..3361
                     /gene="gcvH"
                     /locus_tag="VLR27_12460"
     CDS             2987..3361
                     /gene="gcvH"
                     /locus_tag="VLR27_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HSP04313.1"
                     /translation="MNVPEDLAYSSDHEWIRTDGGRARVGITDYAQDALGDVVYVQLP
                     EVGASVTAGEAIAEVESTKSVSDIYAPVSGTIVEVNADLADTPERLNDDPYGEGWLFI
                     IETADGATELLDAAAYRNIIEG"
     gene            3371..>3757
                     /locus_tag="VLR27_12465"
     CDS             3371..>3757
                     /locus_tag="VLR27_12465"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HSP04314.1"
                     /translation="MADHVFCTQCGHGNPLGSNFCSSCGAGLEHRRGEDSTITFHTEP
                     ALEGSEDVGIDLHEIPAGVGALVVKRGRNAGSRYVLDADKTVAGRHPESDIFLDDVTV
                     SRRHAEITRDGDAYTVRDVGSLNGTYL"
BASE COUNT          545 a         1356 c         1313 g          543 t
ORIGIN      
        1 gcgatcgagg tcctcggggc ggggaccgtc gccgggggag gggccgaccc gctcgaggac
       61 gtcgacgaag gaccgccgga tcttctcgcg ggcgccggtg agggtgccca tgccgacggc
      121 gaggagcgac ccggcgacgg cagcgggcgc cgccaacagg ccggggagca tcccggcggt
      181 gtcgacaccc tcccgatact cgaagtcgct cgcatggatg gtgtcgccgc tgtcccgcag
      241 gagcgacgcc gagcggtgga ccaccggcgg gttcaggaac ggcgagggca ccatcggcga
      301 cagccaacgg ccggtgccgc tgtgcttgcg gggccgcagg tcgtagcgac cggagtgatc
      361 gccgctggtg tcgagcacgt gggggtcgga gacctctggc cgaccccgcc ggatctgctc
      421 gagcccgctg gcgaacgtgc cgccggagac cgcatcggcg gccgagcgca cccgcaggtc
      481 ccgctcggag gtgatggtga cggcgacgtc gaccgaggcc accccgagcc cggtgcgctc
      541 gtgcagcagg tgggcggcga ggagcgcccc gaggtcgaag ggcagtgcct cgaagcccgc
      601 ggtggggacg atgatcgagc cgctctcggt cgcccggtcg tcgtagcggc ggatcatgtc
      661 gagcacccag tcgatctccc cgcacgggtc gatgcagtgg gtgccggccc gcgcgcacgc
      721 cgcgtagacc ggctcgccgt ggcggacgaa ggggccgacc aggtcggcga ccaccttcgc
      781 ggcaccgacc atggcctcga tggaggcccg gtcgtcggtg tcggcgtgga ggatgtcggc
      841 cggttcggct ccgaccgacg ccagcgagcg ctcgatgcgg tcgcggtcac gtccggcgac
      901 ggcccaggtg agcccgagct cgggggctcg gcggtgcagg tggcgggcca cctcgcggcc
      961 ggtgacaccg gtgggcccga acaggacgac gtcgaacgct ggggtcgcat cggtcatgga
     1021 cggaagcgtg gcaggccacc gcggcggtcg ccaccgtggc taccgtcggt gccatgtgcg
     1081 gtcgcttcgt gtccacgtcg ccgcccgacg agctggcggc ctacttcggt gccgaggccg
     1141 tggccgagtc ggtcctcgag cccaactaca acgtcgcccc gacccaggac gtcctggccg
     1201 tcgtcgaggc cgacggtggc cgcttcctgg acgccttctt ctggggcctc gtgccgtcgt
     1261 gggccaagga cgtcaagatc ggctcgcgca tgatcaacgc gcgtgccgag acgctggcgg
     1321 agaagtcggc cttcaagaag cccttcgccg cccgccgatg cctggtgccc gccgacggct
     1381 tctacgagtg gcgcaagctc gacagctcca agaagcccaa gaagcagccc atgttcatcc
     1441 accgcagcga cggcgagccg ctggccttcg ccgggatctg gtcggtgtgg cggggcccgg
     1501 acaaggacca ggaaccgctg cgctcgctgt cgatcgtgac cacctcaccc aacgagctca
     1561 tggccaccat ccacgaccgc atgccggtga tcctgcccga ggagcggtgg gccacctggc
     1621 tcgaccgcga caaccacgac ctcgactcgc tcaccgacct gctcgtgccg ctcccggacg
     1681 ggctgctcac ggcgacgccg gtgtccaccc tggtgaacaa cgtgcgcaac aacggtcccg
     1741 agctcctgga gccggaggcc gtggagcaga ccacgctgct ctgagtgtcg ccggaggcga
     1801 cactcagacg ccggggtcgt ggccggcgtc gcgcagcgca gcgacgaggt cgtcgcgtgc
     1861 ctggtccatg gcggcggcat ccgggtcgcg cgccgcgttg gcgaagtcca tgtcgtgcac
     1921 gccgtcgatc ggcacgacgt gcaggtggac gtgggggacc tcgaggccgg cgatcatgag
     1981 gcccacgcgg gtgggggaga aggccgccat ctgcgcctgc ccgatccgct ggctcacctc
     2041 catcaggtgg ctgttgagct cggggtcgag gtcgagccag tggtcgacct cgacgatcgg
     2101 cacgaccagg gcgtggccgt ggcgcagggg gttgatggag aggaacgcca cgcagcgatc
     2161 gtctcgccag acgaagcggc cggggagctc gccttcgatg atcttggtga acacggtggc
     2221 catgcccgcc agtgtgacag ggaccgtagg gtggatttca tgagcgaacc ggtcgaggac
     2281 gccgccgaat ccgctgtcgc acccgacgac gcgatcatcc tcgacgagga cgtcgtcgaa
     2341 gacgccgggc tcctcaccat ccccaacctc atcaccgtcg tccggctgtg cacgctgccg
     2401 gtcttcgtct acctcctgtt cggcgccgac gaccggtggg cggccggctg ggtgctgttc
     2461 ctcatcggga gcacggactg gctcgacggc tacctcgccc gccgcctcgg ccaggtctcc
     2521 aacgtcggca agatcctcga ccccgtcgcc gatcgactgc tgttcttcgt gggcatcggc
     2581 tcgatcatca tcgacggctc ggtcccgctc tggttcgccg tcgccgtcct ggtccgggag
     2641 gccgcggtcg ccatcggcac gctctcgctc gccgcgctcg gtgccactcg gatcgacgtc
     2701 acctggtacg gcaaggcggc gacgttcgcc ctcatgggtg cgttcccgtc gttcctcgtc
     2761 ggggccagcg accacgccac cgctgacggc ttccgcgtcc tggcctgggt cgtcggcatc
     2821 cccggcctcg ccctcgccta ctggtccgcc ctcctctaca tcccgctcgg ccgcaaggcc
     2881 ctcgccgagg ggcgccaggc ccgcctcgac cgcaccagcg cctgatcggg ccggtggttg
     2941 ctccatcacg gagccggagc aggatcgtcc ggtagcctcc gcgggcatga acgtgcccga
     3001 ggacctcgcg tactcgtccg accacgagtg gatccgcacc gacggcggtc gggcccgcgt
     3061 cggcatcacc gactacgccc aggacgccct gggtgacgtg gtctacgtcc agctgcccga
     3121 ggtcggtgcc agcgtgaccg ccggcgaggc catcgccgag gtcgagtcga ccaagtcggt
     3181 ctcggacatc tacgcgccgg tgtcgggcac catcgtggag gtcaacgccg acctcgccga
     3241 caccccggag cgcctgaacg acgaccccta cggcgagggc tggctcttca tcatcgagac
     3301 cgccgacggc gccaccgagc tcctggacgc cgccgcctac cggaacatca tcgagggctg
     3361 agcgtctccc atggctgatc acgtcttctg cacccaatgc gggcacggca acccgctcgg
     3421 ttcgaacttc tgctcgtcct gcggggcggg cctcgagcac cgtcgcggcg aggactccac
     3481 gatcaccttc cacaccgagc ccgcgctcga gggctccgag gacgtcggca tcgatctcca
     3541 cgagatcccc gccggagtcg gcgcactggt cgtcaagcgc ggtcgcaacg ccggtagccg
     3601 ctacgtcctg gacgccgaca agaccgtcgc cggccgccac ccggagagcg acatcttcct
     3661 cgacgacgtc accgtctccc gccgccacgc cgagatcacc cgcgacggcg acgcctacac
     3721 cgtgcgcgac gtcgggtcgc tcaacggcac ctacctg
//
LOCUS       DATISU010000316         9166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_302153, whole genome shotgun sequence.
ACCESSION   DATISU010000316 DATISU010000000
VERSION     DATISU010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9166
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302153"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VLR27_12470"
     CDS             complement(<1..518)
                     /locus_tag="VLR27_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04315.1"
                     /translation="MTAQRRATLDVLVGDIRTDLGAAHRAREVALPACRTAIRHAGLA
                     IRAVHRDDPTAHDEQVAASEAALRTAQDALADHPMVAHAGFLHDAEKEYAEARLCAAM
                     VRGEALASPAELGVMAHSWMRGLAEAASELRRNVLDRLRAGDLEGGEALLEVMDDAYD
                     VLASVDLPDALTG"
     gene            complement(628..1089)
                     /locus_tag="VLR27_12475"
     CDS             complement(628..1089)
                     /locus_tag="VLR27_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04316.1"
                     /translation="MTIMVLVVLAAIWAAVLLPPYLQKRRAFHPHASIGDFRNQLAVL
                     QRTGDPYAPAPMRTPSGRTMSRTDAQRRRRDVLATLAASAVLTFLLAVVAGGSVWLLH
                     LSVDAALLGYVALLMQIQQQRQAPARTNVAYLAAQNRPSATQQALLRRQAN"
     gene            complement(1305..1811)
                     /gene="moaC"
                     /locus_tag="VLR27_12480"
     CDS             complement(1305..1811)
                     /gene="moaC"
                     /locus_tag="VLR27_12480"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HSP04317.1"
                     /translation="MSERGLTHLDPLGRARMVDVTPKDPTHRRAIARGRVTMSPETAS
                     LVARGAIAKGDVLAVARVAGIQAAKRTPDLIPLCHPLLVGSVLVNFRIEDDFIEVEAQ
                     VDTIDRTGVEMEAMTACSIACLTIYDMCKSADRAMVISELTLWEKTGGRSGTYRRQPE
                     PGEDGLER"
     gene            complement(1839..2864)
                     /gene="moaA"
                     /locus_tag="VLR27_12485"
     CDS             complement(1839..2864)
                     /gene="moaA"
                     /locus_tag="VLR27_12485"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HSP04318.1"
                     /translation="MTRPTTPEQLVDGFGRVHRDLRISVTDRCNFRCTYCMPEEGMQW
                     LDRKEILSFEEITRIARVFVERYGVDSIRLTGGEPTVRAHLPVLVEKLAALGVELAMT
                     TNGSTLRAQGQALADAGLRRINVSLDSLRRDRFLELTRRDELAKVLDGIDAAKEAGLD
                     PVKVNVVLVGGVNDDEVVDFARFGRDNDVTVRFIEFMPLDADGGWEHAQVVPQAEIVR
                     RIAEVFPMEPIVRGHEPAEQWRYLDRPAGRPGASAGDVVGVIPTVTRPFCESCDRVRL
                     TAEGQLRNCLFALDEFDLRAPLRDGGTDDDLAAVMQRAVGAKWAGHAIGNVQFIRPRK
                     SMSQIGG"
     gene            complement(2911..8052)
                     /locus_tag="VLR27_12490"
     CDS             complement(2911..8052)
                     /locus_tag="VLR27_12490"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HSP04319.1"
                     /translation="MRFGVLGPVQVWTPAGIADVGGARQRRLLAALVLHAGEVASTDR
                     LLDIVFEGAPPSGASTTIRTYVARLRKSLGDAEAGAEQLITTEQGGYRLQIDLDAIDS
                     ARFEASIETARGQFDDRDPIGAAATLREGLAMWRGAAFGEFTFEEWARPEASRLEELK
                     TVAEEELNEALLACGLAHDVVSATRAQIQEHPLREELRGQHMLALYRAGRQVEALRSL
                     EEFQSVLVDVGLEPSEDLLRLGRSIASHDPALRLESPAGQPLRGYRVGAGLGRGAHGV
                     VYRAVQPGVGREVAIKTIRAQLADDPEFIRRFDAEAQLVANLEHPHVVPIYDYWREPG
                     GAYIVMRLLSDNLGSRLAAGPMAVPAVAATARELGGALAAAHRAGVVHGDIKPSNVLV
                     DESSTYLADFGVATLVESASGDETTYPSSGYESPELLAGEPPSPASDQFALAVLLIQL
                     LTGQLPFGTRAIATPHDRAPSVHVQRPSVPAPVDDVLRKATAWDATDRYADVDSFVEH
                     LDAALAGQGAPTPRDRQLANPYRGLRAFSEIDRAVFFGRDALVDELVGCLEGPAPDDR
                     LLVVVGASGSGKSSAVRAGLIPRVRSGAVPGSEHWLIATMQPGQDPFGELEAALRSVA
                     ATDPPQRAGATDESDVIRLLDAAVPPTQPLLLVIDQFEELFTQVSEEATTRHFITGLT
                     RAVRRPDGNLRVVATLRADHLDRPLRYSDFGQLVKRGAVPIVGMSATELASAVTRPAA
                     GVGIEIEPALATQLVADVLDQPAALPLLQFTLTELFERRTGPVLTLAGYRGLGGVDAA
                     VAGRAEAVLGRLTDAQRELARRMFLRLVTVDASGSVSRRRALRSDLLSVVEEPGSMDG
                     VVDAFGRSRLLTFDRDPHSREPTVELAHEALIDQWPRFGGWVADAGAGLRIRDQLAEA
                     AHTWDQLGRDDGDLYRGLRLEGAMEWAGAQPDALSLLEGEFLARSAGARAEEIEAERA
                     QAERDRRTNRRLRGLLAGVGLLLVIALVAGALAVRQQRRAERQTEVAVAAVADADLAT
                     LISRSAAQSAENAELSVLLALEAHRRSPGRETEQAVLNALGSSGIPNRVASISGLDNG
                     DCRPIPTFVGSDGMWEHAVVDGRLASLDLGTGDAGDHGPAPEDCGAWLGDPATGRVVV
                     SSADAERIWVGSFEDPYAIRVEQDSAMYLLDSDPATGVVAFVDPRGGNEAVILVDVVT
                     GERIAGPIGEGTLWAVGVDPSGSFAAVSHRERDTADRGRLRVLDVPTGEERFRLDTTA
                     TATSLTFDPETRELIVGTADGALMTVDPVTGEVTSTVATSATSLVIAVGVRDDGRIVA
                     VTEGQIELVDRRTGSAGAPTELTDVVVARVRPDGTVVTRGADGTYDVIELGGNALVER
                     SWPVDPFARVAFNAGRAGVFTLEGQVLSIVDLATGQQRGLDLRNGEGAPVVVDGVYPE
                     RDGVWALAFAGFAARWEGDRLVERIDFPGRLVAWTRSGDLIAVLHRDADGRQVASLVD
                     LEHDAAGIVFTVPAPDGSSVHPALGGGLHVYDRDGALHSYDADGEPTGVIDTGARETF
                     INTMDPTTGVLAVDGGPSGGVVVVDPASGDVQQVPGNDAVANLGFAQEGRLLVITGFD
                     GTVRIWDLERSESAGLAWNGTGVGPSSPSWYDPSSDSIWVYTSGRLLEVPLDPQRWVE
                     RACEIVGRDLTPEERDRYVPGADDATDRAGGSRLAGASAPRAACA"
     gene            8290..8727
                     /locus_tag="VLR27_12495"
     CDS             8290..8727
                     /locus_tag="VLR27_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04320.1"
                     /translation="MLVHDKRPETSETETTTESTRPPRGRRHGLVALVATGAIAGAAI
                     LAASSGSGTEPTPADRSHEAVEANRTAALRDVATVGRGSHHRVEANRTGALRDLVTWG
                     GALDRVEANRTAALRDLVTSSGPLDVGHEPGAELRRSGSVRPR"
     gene            complement(8714..>9166)
                     /locus_tag="VLR27_12500"
     CDS             complement(8714..>9166)
                     /locus_tag="VLR27_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04321.1"
                     /translation="HWTPPDAMAMNSTKRFATWFFVCRAPVGEDGVVTIDMGEIHEDE
                     WVRPTEMLARRDAGEIQLVPPTVVTLHDLAAHDTVDAALAAARDREAFRYVTKILGGA
                     DGTLVALWHGDAAYESEDLAAAGPRHRLVMADPDWTFEDDVTGHFSVA"
BASE COUNT         1275 a         3234 c         3422 g         1235 t
ORIGIN      
        1 ccggtgaggg catcgggcag gtcgacggag gcgaggacgt cgtaggcgtc gtccatcacc
       61 tcgaggaggg cttcgccgcc ttcgaggtcg ccggcgcgca gccggtccag gacgttgcgg
      121 cgcagctcgc tggccgcctc ggccaggccc cgcatccagg agtgggccat gacgccgagc
      181 tcggccgggg aggcgagcgc ctcgccccgc accatcgccg cgcacaggcg ggcttcggcg
      241 tactccttct cggcgtcgtg gaggaagccg gcgtgggcga ccatcgggtg gtcggccagg
      301 gcgtcctgcg cggtgcgcag ggcggcttcg gaggccgcga cctgctcgtc gtgggcggtg
      361 gggtcgtcgc ggtggacggc gcggatggcg aggccggcgt ggcggatcgc cgtgcgacag
      421 gcgggcaggg cgacctcgcg ggcccggtgg gcggcgccga ggtcggtgcg gatgtcgccg
      481 acgaggacgt cgagcgtggc ccgccgctgg gcggtcacga gttgctcagt ggatcacgca
      541 gcgccgcagg aagtcgtggc ggcttcgcca ccacagggca ccgtgacacg ggtcgcttcg
      601 cctggcggct cagctccgag ccttcgttca gttggcctgg cggcgcagga gcgcctgctg
      661 cgtggccgac ggacggttct gggcggccag gtaggccacg ttggtgcggg ccggggcctg
      721 acgctgctgc tggatctgca tgagcagggc gacgtagccg aggagggcgg cgtcgacgct
      781 gaggtggagc agccagaccg agccaccggc gacgacggcg aggaggaagg tgagcacggc
      841 gctggcggcg agcgtggcga gcacgtcgcg gcggcggcgc tgggcgtcgg tgcggctcat
      901 ggtgcgaccg gagggggtgc gcatcggcgc cggggcgtac ggatcgcccg tgcgctgcag
      961 cacggcgagc tggttccgga agtcgccgat ggaggcgtgc gggtggaacg cccggcgctt
     1021 ctgcaggtag gggggcagga gaacggcggc ccagatcgca gcaagcacca ctagcaccat
     1081 gatggtcacg ctcctcgcct cgctttcagt gtgtcgtgag tcaccgtagg agcgcccgga
     1141 acctgagtgg tggatccggg cggtcggcgg tgttcgttcg gttggcgggc aggccagggg
     1201 gctggcccgg gatcgttacg gttctactac gaaccgcaac acggtgacaa gcaactgcga
     1261 aggatgtcac gcacgatgcg aaccggatcg agcagacgag ccgatcagcg ctcgaggccg
     1321 tcctcgcccg gttcgggctg gcggcggtac gtgccggagc ggccgcccgt cttctcccac
     1381 agcgtcagct cgctgatcac catggcacgg tcggcgctct tgcacatgtc gtagatcgtc
     1441 aggcaggcga tggagcaggc ggtcatcgcc tccatctcca cgccggtgcg gtcgatcgtg
     1501 tcgacctggg cctcgacctc gatgaagtcg tcctcgatgc ggaagttcac cagcacggac
     1561 ccgacgagca gcgggtggca cagcgggatg aggtccggcg tgcgcttggc cgcctggatg
     1621 ccggcgacgc gtgccacggc gagcacgtcg cccttggcga tggccccgcg cgccacgagc
     1681 gacgccgtct cgggcgacat ggtgacccgg cccctggcga tggcccgacg gtgcgtcggg
     1741 tccttgggcg tgacgtcgac catccgggca cggcccaggg gatcgagatg ggtcagtcct
     1801 cgctccgaca tcggccggag tgtagggcgg acgctcggct aaccgccgat ctgggacatc
     1861 gacttccggg gtcggatgaa ctgcacgttg ccgatggcgt ggccggccca cttggcgccg
     1921 accgcacgct gcatcaccgc agcgaggtcg tcgtcggtgc cgccgtcgcg cagcggggcg
     1981 cgcaggtcga actcgtcgag ggcgaacagg cagttgcgca gctggccctc ggcggtgagg
     2041 cgcacccggt cgcaggactc gcagaagggg cgcgtgacgg tcgggatcac gccgacgacg
     2101 tcgcccgcgg acgcgccggg ccggccggcg gggcggtcga ggtagcgcca ctgctcggcg
     2161 ggctcgtggc cccgaacgat cggctccatc gggaagacct cagcgatgcg gcgcacgatc
     2221 tcggcctggg gcaccacctg ggcgtgctcc cacccgccgt ccgcgtcgag gggcatgaac
     2281 tcgatgaagc gcacggtgac gtcgttgtcg cggccgaagc gggcgaagtc cacgacctcg
     2341 tcgtcgttga ccccgccgac cagcaccacg ttgaccttca ccgggtcgag gccggcctcc
     2401 ttggccgcgt cgatgccgtc gaggaccttg gccagctcgt cccgtcgggt gagctccaag
     2461 aaccggtcgc ggcgcagcga gtccaggctc acgttgatgc ggcggaggcc ggcgtcggcg
     2521 agggcctggc cctgggcccg cagcgtggag ccgttggtgg tcatggccag ctcgacgccg
     2581 agggcggcga gcttctcgac cagcacgggc aggtgggctc gcacggtggg ctcgccgccg
     2641 gtgagccgga tgctgtcgac gccgtagcgc tcgacgaaca cccgggcgat gcgggtgatc
     2701 tcctcgaacg agaggatctc cttgcggtcc agccactgca tcccctcctc gggcatgcag
     2761 tacgtgcagc ggaagttgca gcggtcggtg accgagatgc gcaggtcccg gtggacgcgc
     2821 ccgaacccgt cgacgagctg ctcgggcgtg gtgggacggg tcatcagatg acccacggta
     2881 ccggcggggc cggcgggctg cgccggcacc tcacgcacag gcggcacggg gtgccgaggc
     2941 gcccgcgagc cgggagccgc ccgctcgatc cgtcgcgtcg tccgctccgg ggacgtagcg
     3001 gtcccgttcc tccggagtca ggtctcggcc gacgatctcg caggcgcgct cgacccagcg
     3061 ctgcgggtcg agcgggacct cgagcaggcg ccccgacgtg tagacccaga tcgagtcgct
     3121 cgacgggtcg taccacgagg gcgacgacgg cccgacgcct gtcccgttcc aggcgaggcc
     3181 ggcggactcg ctgcgctcga ggtcccagat gcgcaccgtt ccgtcgaagc cggtgatcac
     3241 gaggagccgt ccctcctgcg cgaagccgag gttggcgacc gcgtcgttgc ccggcacctg
     3301 ctggacgtcg ccgctcgccg gatcgacgac aacgacgccg ccggaggggc ctccatcgac
     3361 agcgagcacc ccggtcgtcg gatccatcgt gttgatgaac gtctcccggg cgccggtgtc
     3421 gatcaccccg gtgggctcgc cgtcggcgtc gtagctgtgc agcgcaccgt cccggtcgta
     3481 cacgtgcagc ccgcctccca gtgccggatg gacgctcgac ccgtccggag cgggcaccgt
     3541 gaagacgatc ccggccgcgt cgtgttcgag gtccaccagc gacgcgacct ggcgtccgtc
     3601 ggcgtcgcga tggagaacgg cgatcaggtc gccggaccgg gtccacgcaa cgagccgccc
     3661 cgggaagtcg atccgctcga ccagtcggtc accctcccac cgcgccgcga atccggcaaa
     3721 ggcgagcgcc cacacgccgt ctcgctccgg gtagacgccg tcgaccacga caggcgcgcc
     3781 ctcgccgttg cggaggtcga gccccctctg ctgtccggtg gcgagatcga cgatcgacag
     3841 cacctggccc tccagggtga agacgccggc gcggccggcg ttgaacgcga ccctggcgaa
     3901 cgggtcgacc ggccagctcc gttcgacgag cgcgttgccg ccgagctcga tgacgtcgta
     3961 cgtcccgtcc gcgccgcgcg tgacgacggt gccgtcgggg cgcaccctcg ccaccacgac
     4021 gtcggtcagc tcggtggggg ctccggcgga tccggtgcgg cgatcgacga gctcgatctg
     4081 cccctcggtc accgcgacga tccggccgtc gtctcgcacg ccgaccgcga tcaccagcga
     4141 ggtcgcggat gtcgcaacgg tcgaggtgac ctcgcccgtc acgggatcga cggtcatcag
     4201 tgccccgtcg gccgtgccga cgatcagctc gcgggtctcc gggtcgaagg tgaggctcgt
     4261 cgcggtcgcc gtggtgtcga gccggaagcg ctcctcgccg gtcgggacgt cgagcacgcg
     4321 gaggcgacct cggtcggcgg tgtctcgctc gcggtggctg accgcggcga acgacccgga
     4381 cggatcgacc ccgaccgccc agagcgtgcc ctcgccgatc gggccggcga tccgctcgcc
     4441 cgtgacgacg tcgacgagga tcacggcctc gttgccgccc cgggggtcga cgaacgcgac
     4501 gacgcccgtc gcggggtcgc tgtcgaggag gtacatggcg ctgtcctgtt cgacccggat
     4561 cgcataggga tcctcgaagg acccgaccca gatgcgctcg gcatccgcgc tgctcacgac
     4621 gacccggccc gtggccgggt ccccgagcca cgcgccgcag tcctccggcg ccggtccgtg
     4681 atcgccggcg tcgcccgtcc ccaggtcgag gctcgccaga cggccgtcga cgactgcgtg
     4741 ctcccacatc ccgtcggaac ccacgaaggt ggggatcggg cggcagtcgc cgttgtcgag
     4801 ccccgagatc gaggcgaccc ggttcgggat cccgctcgac ccgagcgcgt tgagcacggc
     4861 ctgctcggtc tcccgtccgg gcgaccgacg gtgggcctcg agcgcgagca gcacggacag
     4921 ctccgcgttc tcggcgctct gcgctgccga ccggctgatg agggtcgcca ggtcggcatc
     4981 cgcgactgcc gccaccgcga cctcggtctg gcgctcggcc cgccgctgct gccggaccgc
     5041 cagcgcgccg gccaccagtg cgatcacgag cagcaggccc acgccggcga gcaggccgcg
     5101 caggcgccgg ttcgtccggc ggtcgcgctc ggcctgcgct cgctcagctt cgatctcctc
     5161 ggcccgggct cctgcgctcc tggcgaggaa ctccccctcc agcaggctga gcgcgtcggg
     5221 ctgcgcaccg gcccactcca tggcgccctc cagtcgcagc ccgcggtaga ggtcgccgtc
     5281 gtcgcgaccc agctggtccc aggtgtgggc cgcctcggcg agctgatcgc ggatccggag
     5341 gccggcaccg gcgtcggcca cccagccacc gaaccggggc cactggtcga tcagggcctc
     5401 gtgggcgagc tcgaccgtcg gctcccggct gtgggggtcg cggtcgaagg tgagcagccg
     5461 cgacctgccg aaggcgtcga cgacgccatc catcgacccc ggctcctcca ccacggaaag
     5521 gaggtcggag cgcagcgccc gacgccggct caccgagccc gaggcatcca cggtcaccag
     5581 gcggaggaac atccggcgcg ccagctcgcg ctgcgcgtcg gtgagccgac cgagcacggc
     5641 ctcggcccgc ccggcgaccg cagcgtcgac cccaccgagg ccgcggtagc ccgccagggt
     5701 cagcaccggc ccggtccggc gctcgaagag ctcggtcagg gtgaactgga ggagcggcag
     5761 cgcggcgggt tggtcgagca cgtcagccac gagctgggtc gcgagcgcgg gttcgatctc
     5821 gatgccgaca cccgcagccg gacgggtgac cgccgatgcg agctccgtcg cagacatccc
     5881 gacgatgggc accgcgccgc gcttcacgag ctgcccgaag tcggagtagc ggagcggtcg
     5941 gtcgaggtgg tcggcgcgca gcgtggccac gacccggagg ttgccgtcgg gacggcggac
     6001 cgctctggtg agaccggtga tgaagtgacg ggtcgtcgcc tcctcgctca cctgggtgaa
     6061 gagctcctcg aactggtcga tcacgaggag gaggggctgg gtcggtggca cggcagcgtc
     6121 gaggagccgg atgacgtcgg actcgtcggt ggcgccggcc cgctggggtg gatccgtcgc
     6181 cgcgacggac cgcagcgcag cttcgagctc gccgaagggg tcctggcccg gctgcatcgt
     6241 cgcgatcagc cagtgctccg agccggggac ggcgccgctg cggacccggg ggatgagccc
     6301 ggcgcggacg gcactcgact tcccgctccc cgaggctccg accaccacga ggaggcggtc
     6361 gtcgggagca ggtccctcca ggcatccgac gagctcgtcg accagcgcgt cgcgaccgaa
     6421 gaagaccgca cggtcgatct cgctgaacgc gcggaggccc cggtacgggt tggccagctg
     6481 gcgatcccgc ggggtcggcg ctccctggcc ggcgagcgcg gcgtcgaggt gctcgacgaa
     6541 ggaatcgacg tccgcgtacc gatccgtggc gtcccacgcg gtggccttcc ggagcacgtc
     6601 gtcgacgggg gcggggacgc tcggccgctg cacgtggacc gacggggccc ggtcgtgcgg
     6661 cgtggcgatg gcacgggtcc cgaacgggag ctggccggtg aggagctgga tgagcaggac
     6721 cgccagcgcg aactgatcgc tcgccggcga cggaggctcg ccggcgagca gctccggtga
     6781 ctcgtagcct gatgacggat acgtcgtctc gtcgccgctc gccgactcga ccagggtggc
     6841 cacgccgaag tcggcgaggt acgtgctcga ctcgtccacg aggacgttgc tcggcttgat
     6901 gtcgccgtgc accacaccgg cccgatgggc tgcggcgagc gcacccccga gctcgcgggc
     6961 ggtggcggcc accgccggga ccgccatcgg accagcggcg agtcgtgacc cgaggttgtc
     7021 cgacaggagg cgcatgacga tgtaggcgcc gccgggctcg cgccagtagt cgtagatcgg
     7081 gacgacgtgg gggtgctcga ggttggcgac gagctgtgct tcggcgtcga agcgtcggat
     7141 gaactcgggg tcgtcggcga gctgggcgcg gatcgtcttg atcgccacct cgcgacccac
     7201 gccgggctgc accgcccggt acacgacgcc gtgcgcgcct cggccgagcc ccgcgccgac
     7261 gcggtagccg cggagcggct gaccggccgg ggattcgagg cgcagggccg ggtcgtggga
     7321 cgcgatgctg cgccccagcc ggagcaggtc ctcggacggt tcgagaccca cgtcgacgag
     7381 cacgctctgg aactcctcga ggctccgcag cgcctcgacc tggcggccgg cgcggtagag
     7441 cgccagcatg tgctgacccc gcagctcctc acggagcgga tgctcctgga tctgggcacg
     7501 cgtcgccgag acgacgtcgt gcgccagccc gcaagcgagg agcgcctcgt tgagctcctc
     7561 ctcggcgacc gtcttcagct cctcgagccg cgacgcctcc ggtcgcgccc actcctcgaa
     7621 cgtgaactcg ccgaatgcgg cgccccgcca catcgccagc ccctcacgga gcgtcgccgc
     7681 agcaccgatc ggatcccggt cgtcgaactg accgcgagcg gtctcgatcg atgcctcgaa
     7741 ccgcgcggag tcgatcgcgt cgagatcgat ctgcaggcgg tagcccccct gctcggtggt
     7801 gatcagctgc tccgcacctg cttcggcatc gccgagcgac ttgcggaggc gggcgacgta
     7861 ggtgcggatc gtcgtcgacg cacccgacgg cggtgcgccc tcgaagacga tgtcgagcag
     7921 ccgatcggtc gacgcgacct cgcccgcatg cagcacgagc gccgccagca gccgccgctg
     7981 acgggcacca cccacgtcgg cgatgcctgc gggcgtccac acctgcaccg gtccgagcac
     8041 cccgaagcgc atcgccaccc cctccgatcg atcctaagca gcggtcggtg gtgagtgaac
     8101 cccgccgacg ttgcatcgac gttgcagcgc gaaaccccag gtcaacgggg tcgtggagca
     8161 gacagacacg tgctccgggg gccgggcggt ggccacggtg cgaccgctcg caccctcgat
     8221 gcaacggccg ggtgcgacgt tcggcgtggc tcaggacgag cccacgcccg acacgaaagg
     8281 cagagaacga tgttggtcca cgacaagcgc ccagagacct ccgagaccga gacgaccacg
     8341 gagtccacgc gtccaccgcg tggtcgacgc cacgggctcg tcgccctggt ggcgacgggc
     8401 gccatcgccg gcgccgcgat cctcgcggca tcgtccggct cgggcaccga gcccaccccc
     8461 gcggaccgct cccacgaggc ggtggaagcc aaccggaccg ccgccctgcg ggacgtggcg
     8521 acggtcgggc gcggttccca ccaccgggtg gaggccaatc gcaccggcgc gctgcgcgac
     8581 ctggtgacgt ggggtggcgc cctcgaccgg gtggaggcca atcgcacggc cgccctgcgg
     8641 gatctggtga cctcgagcgg ccccctcgac gtgggccacg agcctggggc cgagctccga
     8701 cgctccggca gcgtcaggcc acgctgaagt gtccggtgac gtcgtcctcg aaggtccagt
     8761 ccgggtctgc catcacgagc cggtgccggg gtccggcggc ggcgaggtcc tcggactcgt
     8821 aggcggcgtc gccgtgccag agggcgacga gggtgccgtc ggcgccgccg aggatcttgg
     8881 tgacgtagcg gaacgcctcc cgatcgcggg ccgcggcgag ggcggcgtcc acggtgtcgt
     8941 gggcggcgag gtcgtggagc gtgacgaccg tgggcgggac cagctggatc tcgccggcgt
     9001 ctcggcgggc cagcatctcc gtgggccgca cccactcgtc ctcgtggatc tcgcccatgt
     9061 cgatggtgac gacgccgtcc tcccccaccg gggcccggca cacgaagaac cacgtggcga
     9121 agcgcttggt ggagttcatc gccatcgcgt ccggcggcgt ccagtg
//
LOCUS       DATISU010000317         1931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_813745, whole genome shotgun sequence.
ACCESSION   DATISU010000317 DATISU010000000
VERSION     DATISU010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813745"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1067
                     /locus_tag="VLR27_12505"
     CDS             57..1067
                     /locus_tag="VLR27_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04322.1"
                     /translation="MNEPRDTLVGDALRRIDVPDHAPEFWSRLDAELAAGPTGPAANN
                     GEGDADADVVELASAPSRRATWARRPVLLAAAVVAIVALAVGAAVLRPGGDDESQLDV
                     AETPGEVDPEPDATETPAPETSAPPEATDPPETTSTTVPEALDAGEAEAVAATWLESL
                     FAGDIDGAYDQLDLTSQSLLDRAGFEELSSGLAEGAAAFSQPGITRAVAVVDAADASF
                     AVVTFTGDVEREGMVETASYPVVVTASGVHFVVDGPQLELDPEYARSSGTTLAAPLEV
                     LVSETADTWLWIDDQEPERFLSPGRLTLGAEAIGPGTHVVSLVAIEGGAITARSYTVV
                     VP"
     gene            1109..1558
                     /locus_tag="VLR27_12510"
     CDS             1109..1558
                     /locus_tag="VLR27_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04323.1"
                     /translation="MVLVFAIVGALLVFAIAAAFVGSETFRLGHTPTTTVFDMDEAVI
                     HVAENLSVEAAMGLTMDEVKQLIQFSLQHLRSRGLSAMPGEELDHDVERAPVVVADDE
                     AVAVVLGRADDVGMEVTDAAVYEVLDLLLGHLVQIGAVGPAVPPPEL"
     gene            complement(1680..>1931)
                     /locus_tag="VLR27_12515"
     CDS             complement(1680..>1931)
                     /locus_tag="VLR27_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04324.1"
                     /translation="VLVVDDEGALVAQPPVLFDPNEAIRHAGHDTRDGEAPGNAGTSP
                     FQFVDRATVVVCTGWDQLPADSLGGGLVGFHQVGPRGLT"
BASE COUNT          274 a          704 c          674 g          279 t
ORIGIN      
        1 cgcgccctgc gcgccgtgct cgaacccacc cgctccgatc ggcatggaga gatccgatga
       61 acgagccccg agacaccctc gtcggtgacg ccctgcgccg catcgacgtc cccgaccacg
      121 cccccgagtt ctggagccgc ctcgacgccg agcttgcagc cggaccgacc ggtccggccg
      181 cgaacaatgg cgagggcgac gccgatgctg acgtcgtcga gctcgcctcc gcaccctccc
      241 gccgtgccac gtgggctcgg cgcccggtgc tgctggccgc cgcggtcgtg gcgatcgtcg
      301 cgctggccgt cggcgcggcc gtgctgcgcc ccggcggtga cgacgaatcc cagctcgacg
      361 tggccgagac accgggcgag gtcgaccccg agcccgacgc gaccgagacg ccggcgccgg
      421 agacctccgc gccgccggag gcgaccgacc caccggagac gacgagcacg acggtgcccg
      481 aggccctcga tgcgggcgag gccgaggcgg tcgccgccac gtggctcgag tccctgttcg
      541 ccggggacat cgacggggcc tacgaccagc tggacctcac gtcgcagtcc ctgctcgacc
      601 gagccgggtt cgaggagctg tcctccgggc tggccgaagg ggcagcagcc ttctcccagc
      661 ccggcatcac ccgtgcggtc gcggtcgtgg acgccgccga tgcgtcgttc gccgtcgtca
      721 ccttcaccgg cgacgtcgag cgggagggca tggtcgagac ggccagctac ccggtcgtcg
      781 tcacggcgtc cggcgtccac ttcgtcgtcg atggcccgca gctcgagctc gacccggagt
      841 acgcccggtc gtccgggacg acgctggctg cgccgctcga ggtgctcgtc tcggagaccg
      901 ccgacacctg gctctggatc gacgaccagg agccggagcg cttcctgtcg ccgggccggc
      961 tgacgctcgg tgcggaggcg atcggcccgg gcacgcacgt cgtctcgctc gtggcgatcg
     1021 aagggggcgc catcaccgcc cggagctaca ccgtggtggt gccctgatcg accagggagc
     1081 acgaggaccg cgtcgctagc gtgccggcgt ggtcctcgtc ttcgcgatcg tcggtgcgct
     1141 gctggtcttc gccatcgctg cggcgttcgt cggctccgag accttccggc tgggccacac
     1201 gccgaccacg acggtcttcg acatggacga ggccgtgatc cacgtcgccg agaacctcag
     1261 cgtcgaggcc gccatgggcc tgaccatgga cgaggtcaag cagctcatcc agttctcgct
     1321 ccagcacctg cggtcccgag gcctgagcgc catgccgggc gaggagctgg atcacgacgt
     1381 cgaacgggcg cctgtggtcg tggccgacga cgaagccgtg gcggtcgtgc tcgggagggc
     1441 cgacgacgtc ggcatggagg tcaccgacgc cgccgtctac gaggtgctcg acctgctgct
     1501 cggccacctc gtgcagatcg gtgcggtggg tccggccgtc ccgccgccgg agctctgagc
     1561 ggtccggaaa gtcgcgatcc gcgagccgcg tgacggcgct agcatttctc gcaccagaga
     1621 agggaggtgg tccaacacat gtacgacaat cgtcttggga ccctggaggt ggctggccgc
     1681 taggtcagtc ctctcggacc gacttggtgg aatcccacca agccaccgcc gagcgaatcg
     1741 gccgggagct ggtcccaccc ggtgcacacc acaacggtcg ctcggtccac gaactggaac
     1801 ggggaggtgc cggcattgcc cggcgcctcc ccgtcccgcg tatcgtgccc ggcatgtctg
     1861 atcgcctcgt tcggatcgaa gagcaccggt ggctgggcga caagcgctcc ttcgtcgtcc
     1921 acgaccagga c
//
LOCUS       DATISU010000318         7195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_674518, whole genome shotgun sequence.
ACCESSION   DATISU010000318 DATISU010000000
VERSION     DATISU010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674518"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VLR27_12520"
     CDS             complement(<1..447)
                     /locus_tag="VLR27_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSP04325.1"
                     /translation="MIGNLDSAVPPGDIGDKWTQHKFDMKLVAPNNRRKFEVIVVGTG
                     LAGAAAAATLGEQGYKVKAITYHDSPRRAHSIAAQGGINAAKNYKNDGDSVYRLFYDT
                     VKGGDYRSREANVHRLAEVSGDIIDQCVAQGVPFAREYGGMLDNRSF"
     gene            complement(444..1382)
                     /locus_tag="VLR27_12525"
     CDS             complement(444..1382)
                     /locus_tag="VLR27_12525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HSP04326.1"
                     /translation="MQTMEQSASSGGAGTGVEPVNPPRRPAPWPVQFYRSAVGKKWVM
                     AITGIMLMGFVFGHMVGNLKMYQGAHALNVYGETLRELLYPLLPRTLTLWIIRTGLAL
                     AFIFHIHAAASLTLMNRRANAGGYQSKRDWQAANTASRSMRFTGIVILAYLIWHLADF
                     TWGWVNPDFVRGDVYRNVQASLTNVIPAAIYVLATLALGVHLFHGAWSLFQSLGLNNP
                     KYNSWRRGFALGFAALITIGNLSFPLAVVSGAASDDVCYEEGDRIVTCETVFAEAIED
                     GRLSIEQYDELSDGERLKIIKAYEANEELRFEEGTS"
     gene            complement(1432..3561)
                     /locus_tag="VLR27_12530"
     CDS             complement(1432..3561)
                     /locus_tag="VLR27_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04327.1"
                     /translation="MTLPDRWLAAPADEDLRRTFADPELDDSGWEPIAVPGHWRSTPA
                     FAEHDGPLLHRTRFEQAPGTGDRRWWLRFDGLFYEGDVWLDGTYLGATEGYFFAHEFE
                     ITEQLRARSEHQLAVEVACSPQTNRAHKRNITGVFQHWDCLDPDWNPGGIWRPVHVEE
                     SGPVRIRHLVTRCLEATPARAVLSVTADLDATEATEVHLRTTVGAADHGTDRTLAAGA
                     NTVEWTVTVERPRLWWPHALGDAHLEQATVEVRLRDAVGSRVSDRRRFRTGLRQVSMR
                     KWVLSVNGERLFCKGANLGPTRMALGEATPDELAADVEAAKATGLDLLRVHGHVGRPE
                     TYDAADRAGMLLWQDFPLQWGYARSIRRQAQRQATEMVAMLAHHPSVAIWCGHNEPMG
                     LDISPEAFTEPHRLAGLGLRALVSQQLPTWNKTVLDRTVKRAIEAADGTRPVIKHSGV
                     LPRLPKLEGTDSHLYFGWYWGDERDLPGFARAMPSQVRFPTEFGAQAVPETDGFMGSE
                     AWPDLDWERLGRTHNLQLALLDRHVPREGRTYEEWKDATQAYQAAVVRHHVEALRRLK
                     YRPTGGFCQFAFADSHPGVTWSVLDHEREPKAAHAALTEACRPVIVVADRPPATVAPG
                     QALALDVHVVSDRRVDLTGTVEVQASWDGGAHRWAFTGDVPADSCVLVGTIQLEVPHA
                     AGELVIALTGEVGEDRPTNEYRTTITT"
     gene            3623..5038
                     /locus_tag="VLR27_12535"
     CDS             3623..5038
                     /locus_tag="VLR27_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSP04328.1"
                     /translation="MAAPSSLPSTLGALRESGWESRPVKEEVRRNAVTKIRAGEPLFE
                     GVLGYEQTVMPQLENALLAGHDVVFLGERGQAKTRMIRSLTRLLDEWMPVVKGSEIHD
                     DPYHPVSRYARDLVQEMGEDTEIDWVHRDERFGEKLATPDTSIADLIGEVDPIKVAEG
                     RYLSDELTLHYGLVPRTNRGIFAINELPDLSERIQVGLLNVLEERDVQIRGYKVRLPL
                     DVMLVASANPEDYTNRGRIITPLKDRFGSQIRTHYPLDVETEMVIADQEARPLQAEGI
                     TASMPSYMSQVVATLSHLARQSPHVNQRSGVSVRLTVSNHEVLIANAARRALRHGEDV
                     VVPRVSDLDALAASTMGKIEIESLDDGRDEAIAEHLVKAAVLTVFRERTDPAMLRDVI
                     DAFEEGRIVDAGDDISSADLASLAEEMPGLKAQVVGLTDGDESAAAMAAAVEFLLEGL
                     HLGKRLNKEHVGARSSYRARS"
     gene            complement(5043..5783)
                     /locus_tag="VLR27_12540"
     CDS             complement(5043..5783)
                     /locus_tag="VLR27_12540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP04329.1"
                     /translation="MAAADTQDRFVEYQTSRSRRLRNRLVEDNLALVEYYVSRYGKGH
                     MEADDVRQQALMALIKAVERFDPEVGVTFKTFANRTIEGELKRLLRDKGWSVRPPRSL
                     QELAVQARRLEPELTQELGRAPRVQELADRLEESTDRVLEALSAGASYRADSLDAPIG
                     DGTTTKGAMIAGSDGDMGQVETRIDVESLLQGLDERDRTIIEMRFVHRRSQQEIADEL
                     GVSQSYLSRLLRKTLATLREQAEEQMER"
     gene            complement(5770..6171)
                     /locus_tag="VLR27_12545"
     CDS             complement(5770..6171)
                     /locus_tag="VLR27_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04330.1"
                     /translation="MPDLPSRSTAELTVPATTAYLRHVRILAATIADDCAFDVEAIES
                     LRVAVDELCALAMADATDTAELTISITTTEAGIDMAGRCSPVTEEPMLDPIAEQLLRA
                     GSSHHALRLEGDTVHLELTAARPTAGVDGGR"
     gene            6224..6634
                     /locus_tag="VLR27_12550"
     CDS             6224..6634
                     /locus_tag="VLR27_12550"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP04331.1"
                     /translation="MSLQPSTRRFPARRVSSFATLAAVREAFVEWLAGVTTSEERVED
                     LSVVISELGANAVRGTPEGAEPATVSAWVEDGLLVLEVANRVDEITVPDADWDFEDPL
                     RTGGRGLLLVSAFVDDVHVDVEDDVLIIRCTTRL"
     gene            complement(6672..7007)
                     /locus_tag="VLR27_12555"
     CDS             complement(6672..7007)
                     /locus_tag="VLR27_12555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSP04332.1"
                     /translation="MTEPTPTPQDRLQVTVDEGDRTTFVLSGDLDPHTSPYLQERIDA
                     TLDEGATTAVLDVGEVSFVDSAGLRVIADTHRRLMNAGGGLVLRNPSKGLEKLLSVTG
                     LSDHVTIEH"
BASE COUNT          986 a         2420 c         2678 g         1111 t
ORIGIN      
        1 gaaggagcgg ttgtcgagca tgccgccgta ctcgcgggcg aagggcacgc cctgggcgac
       61 gcactggtcg atgatgtcgc cggacacctc ggccaggcgg tgcacgttgg cctcgcggga
      121 gcggtagtcg ccgcccttca cggtgtcgta gaagaggcgg tacaccgagt cgccgtcgtt
      181 cttgtagttc ttggcggcgt tgatgccgcc ctgggcggcg atcgagtgcg cccggcgggg
      241 cgagtcgtgg taggtgatcg ccttcacctt gtagccctgc tcgccgagcg tggcggcagc
      301 ggcggcgccg gcgaggccgg tgcccacgac gatgacctcg aacttgcgcc ggttgttcgg
      361 cgccacgagc ttcatgtcga acttgtgctg cgtccacttg tcaccgatgt cccccggggg
      421 gactgcggag tccaggttgc cgatcacgac gtgccctcct cgaagcggag ctcttcgttg
      481 gcctcgtagg ccttgatgat cttgaggcgt tcaccgtcgc tgagctcgtc gtactgctcg
      541 atgctcaggc ggccgtcctc gatggcctcg gcgaacacgg tctcacaggt gacgatgcgg
      601 tcaccctcct cgtagcagac gtcgtcggag gcggcgccgg agacgacggc gagcgggaag
      661 ctgaggttgc cgatggtgat gagggcggcg aagcccaggg cgaagccccg gcgccagctg
      721 ttgtacttgg ggttgttcag cccgaggctc tggaacaggc tccaggcccc gtggaacagg
      781 tgcaccccga gggcgagcgt ggccagcacg tagatggcag ccgggatcac gttggtgagg
      841 ctggcctgca cgttgcggta gacgtcgccg cggacgaagt ccgggttgac ccagccccag
      901 gtgaagtcgg ccaggtgcca gatgaggtag gcgaggatga cgatgccggt gaagcgcatc
      961 gagcggctgg ccgtgttggc cgcctgccag tcgcgcttgc tctggtagcc gccggcgttg
     1021 gcccggcggt tcatcagggt gaggctggcc gcggcgtgga tgtggaagat gaaggcgaga
     1081 gccagcccgg tgcggatgat ccacagcgtg agcgtgcggg ggagcagcgg gtagagcagc
     1141 tcgcgcaggg tctcgccgta gacgttcagg gcatgggcgc cctggtacat cttgaggttg
     1201 ccgaccatgt ggccgaacac gaagcccatg agcatgatgc ccgtgatggc catgacccac
     1261 ttcttgccga cagccgatcg gtagaactgc accggccacg gcgcggggcg gcgtgggggg
     1321 ttcacgggct cgaccccggt gcccgcaccc cctgacgagg ctgactgctc catggtctgc
     1381 atggattcgc acggtacccg tcgcccgata tcggggggca atcacctcgg atcaggtggt
     1441 gatggtggtg cggtactcgt tggtcggacg gtcctcgccc acctctccgg tgagcgcgat
     1501 gacgagctcg cccgcggcgt gggggacctc gagctggatc gtgccgacga gcacgcagga
     1561 gtcggcaggg acgtcgccgg tgaaggccca tcggtgcgcg cccccgtccc agctcgcctg
     1621 gacctcgacg gtcccggtca ggtccacccg ccggtccgac accacgtgga cgtcgagggc
     1681 cagcgcctgg ccgggtgcga cggtggccgg ggggcggtcg gcgaccacga tcaccggccg
     1741 gcacgcctcg gtgagggcgg cgtgggccgc cttcggctcg cgctcgtggt cgagcaccga
     1801 ccaggtgacg ccggggtggg agtcggcgaa ggcgaactgg cagaagccgc ccgtgggtcg
     1861 gtacttgagg cgacgtagcg cctcgacgtg gtggcgcacg acggcggcct ggtacgcctg
     1921 cgtggcgtcc ttccactcct cgtaggtgcg cccctcgcgt ggcacgtggc ggtcgaggag
     1981 ggcgagctgc aggttgtgcg tgcgacccag gcgctcccag tcgaggtccg gccacgcctc
     2041 cgaacccatg aagccatcgg tctcgggcac ggcctgcgca ccgaactcgg tggggaagcg
     2101 gacctggctg ggcatcgccc gggcgaagcc gggcaggtcg cgctcatctc cccagtacca
     2161 gccgaagtag aggtggctgt cggtgccctc gagcttgggg aggcggggca ggacgccgga
     2221 gtgcttgatc accgggcggg tgccgtcggc ggcctcgatg gcccgcttca ccgtccggtc
     2281 cagcaccgtc ttgttccacg tcggcagctg ctgggacacc agggcccgca ggccgagccc
     2341 ggcgaggcgg tgcggttcgg tgaaggcctc gggggagatg tcgaggccca tcggctcgtt
     2401 gtgcccgcac cagatggcca cggaggggtg gtgggcgagc atggccacca tctcggtggc
     2461 ctggcgctgc gcctggcgac ggatcgagcg ggcgtagccc cactgcagcg ggaagtcctg
     2521 ccacagcagc atcccggccc ggtcggcggc gtcgtaggtc tcgggccggc cgacgtggcc
     2581 gtggacgcgg agcaggtcga ggccggtggc cttggccgcc tcgacgtcgg cggccagctc
     2641 gtccggcgtg gcctccccca gcgccatgcg ggtgggcccc aggttggccc ccttgcagaa
     2701 caggcgctcg ccgttgaccg acagcaccca cttgcgcatg gacacctggc gtaggccggt
     2761 gcggaaccgt cgccggtccg acacgcggct gccgacggcg tctcgcaggc gcacctcgac
     2821 cgtggcctgc tcgaggtggg cgtcgccgag ggcgtggggc caccacagcc gggggcgctc
     2881 gacggtgacc gtccactcca cggtgttggc gcccgcggcc agcgtgcggt cggtgccgtg
     2941 gtcggcggcg ccgacggtgg tgcgcaggtg cacctcggtc gcctcggtgg catcgaggtc
     3001 ggcggtgacc gacagcacgg cgcgggcggg ggtggcctcg aggcagcgcg tgaccaggtg
     3061 gcggatgcgg accgggccgg actcctcgac gtgcaccggc cgccagatgc cgccggggtt
     3121 ccagtccggg tccaggcagt cccagtgctg gaacacgccg gtgatgttgc gcttgtgggc
     3181 gcggttcgtc tgcggcgagc acgccacctc gacggccagc tggtgctcgc tccgggcccg
     3241 gagctgctcg gtgatctcga actcgtgggc gaagaagtac ccctccgtgg cgccgaggta
     3301 ggtgccgtcg agccacacgt cgccctcgta gaagaggccg tcgaagcgca gccaccagcg
     3361 ccggtcgccg gtgcccggcg cctgctcgaa ccgggtccgg tgcaggagcg ggccgtcgtg
     3421 ttcggcgaag gccggcgtcg agcgccagtg gccgggcacg gcgatcggct cccagcccga
     3481 gtcgtccagc tcggggtcgg cgaacgtgcg gcgcaggtct tcgtcagcgg gggcggccag
     3541 ccagcggtcg gggagcgtca tcggcggcgg agcctaaaca cgccgaggtc gacggcctcg
     3601 gggtggccga taggttggcc ccatggcagc cccgtccagc ctcccgtcca ccctcggcgc
     3661 gctccgggag tccggttggg agtcccggcc ggtcaaggag gaggtgcggc gcaacgccgt
     3721 caccaagatc cgggccggtg agccgctctt cgagggcgtg ctcggctacg agcagaccgt
     3781 catgccccag ctggagaacg ccctgctggc cggtcacgac gtggtgttcc tcggcgagcg
     3841 cggccaggcc aagacccgca tgatccgctc gctcacccgg ctgctcgacg agtggatgcc
     3901 ggtggtcaag ggctccgaga tccacgacga cccctaccac ccggtctccc gctacgcccg
     3961 ggacctcgtg caggagatgg gtgaggacac cgagatcgac tgggtccacc gcgacgagcg
     4021 cttcggcgag aagctggcca cccccgacac ctccatcgcc gacctgatcg gcgaggtcga
     4081 cccgatcaag gtcgccgagg gtcgctacct ctccgacgag ctgaccctgc actacggcct
     4141 ggtgccccgg accaaccggg ggatcttcgc catcaacgag ctgcccgacc tgtccgagcg
     4201 catccaggtg ggcctgctga acgtgctcga ggagcgggac gtgcagatcc gcggctacaa
     4261 ggtccgcctg ccgctcgacg tcatgctcgt cgcgtcggcg aaccccgagg actacaccaa
     4321 ccgcggccgc atcatcacgc cgctgaagga ccgcttcggc agccagatcc gcacccacta
     4381 ccccctcgac gtcgagaccg agatggtcat cgccgaccag gaggcccgcc cgctgcaggc
     4441 cgagggcatc acggcgtcca tgccgagcta catgagccag gtcgtggcca cgctgtcgca
     4501 cctcgcccgc cagtcgccac acgtgaacca gcgctccggc gtgtcggtgc gcctcaccgt
     4561 ctccaaccac gaggtgctca tcgccaacgc cgcccggcgg gccctgcgcc acggcgagga
     4621 cgtcgtcgtc ccgcgcgtgt ccgacctcga cgcgctggct gcttcgacga tgggcaagat
     4681 cgagatcgag tccctcgacg acggccgcga cgaggccatc gccgagcacc tggtgaaggc
     4741 agccgtgctc accgtgttcc gcgagcgcac cgacccggcc atgctccgcg acgtcatcga
     4801 cgccttcgag gagggccgca tcgtcgacgc cggcgacgac atctcctccg ccgacctcgc
     4861 ctccctcgcc gaggagatgc ccggcctcaa ggcccaggtc gtcggcctca ccgacggcga
     4921 cgagtccgcc gccgccatgg ccgccgcggt cgagttcctc ctcgagggcc tgcacctcgg
     4981 caagcgcctc aacaaggagc acgtgggcgc ccgctccagc taccgcgcca ggagctgagc
     5041 gactaccgct ccatctgctc ctcggcctgc tcgcgcaggg tggcgagggt cttgcgcagc
     5101 aaccgggaca ggtacgactg ggagacgccg agctcgtcgg cgatctcctg ctggctgcgc
     5161 cggtgcacga accgcatctc gatgatggtg cggtcccgct cgtcgaggcc ctggaggagg
     5221 gactcgacgt cgatgcgggt ctcgacctgg cccatgtcgc cgtcgctgcc ggcgatcatg
     5281 gcgcccttgg tggtggtgcc gtcgccgatc ggggcgtcga ggctgtcggc ccggtaggac
     5341 gcgccggccg agagcgcctc gagcacgcgg tcggtggact cctcgagccg gtcggccagc
     5401 tcctggacgc gcggcgcccg ccccagctcc tgggtgagct cgggctccag ccggcgggcc
     5461 tggacggcca gctcctggag gctgcggggc gggcggacgg accagccctt gtcccgcagg
     5521 aggcgcttca gctcgccctc gatggtccgg ttggcgaagg tcttgaaggt gacgccgacc
     5581 tcgggatcga agcgctcgac cgccttgatg agcgccatca gcgcctgctg gcggacgtcg
     5641 tcggcctcca tgtggccctt gccgtagcgg ctgacgtagt actcgaccag cgcgaggttg
     5701 tcctcgacca accggttgcg gaggcggcgt gaccgcgacg tctggtactc gacgaagcgg
     5761 tcctgggtgt cagcggccgc catcgacgcc agccgtgggc cgtgccgcgg tgagctcgag
     5821 gtggacggtg tcgccctcga ggcgcagggc gtggtgggac gagccggcac gcagcagctg
     5881 ttcggcgatc gggtcgagca tcggctcctc ggtgacgggg ctgcagcgcc cggccatgtc
     5941 gatgccggct tcggtcgtgg tgatcgagat cgtgagctcg gcggtgtccg tggcgtcggc
     6001 catggcgagg gcgcacagct cgtcgacggc gacccgcagg gactcgatgg cctccacgtc
     6061 gaaggcgcag tcgtcggcga tggtggccgc caggatccgc acgtggcgga ggtaggccgt
     6121 ggtcgccggg acggtcagct cggctgtgga tcggctgggg aggtcaggca tgatcgttcc
     6181 gttctacagt gctgagggcg cgggtacgtg cccgttgatc gagatgtcct tgcaaccttc
     6241 cacccgccgt tttcctgcgc gacgtgtgtc gtcgttcgcc actctcgccg cggtgcgtga
     6301 ggcgttcgtg gagtggctcg ccggcgtgac cacgtcggag gagcgggtcg aggacctctc
     6361 cgtcgtgatc agcgagctcg gcgccaacgc cgtgcgcggc acgcccgagg gcgccgagcc
     6421 ggccacggtc agcgcctggg tcgaggacgg gttgctcgtc ctcgaggtcg ccaaccgcgt
     6481 cgacgagatc accgtgccgg atgcggactg ggacttcgag gacccgttgc gcaccggcgg
     6541 ccggggcttg ctgctcgtgt cggcgttcgt ggacgacgtc cacgtcgacg tcgaggacga
     6601 cgtgctgatc atccgctgca ccacccggct ctgagcgccc gctcggtggg gcgctgtcgc
     6661 gcccgccggc atcagtgctc gatggtgacg tggtcggaga gacccgtcac ggacagcagc
     6721 ttctccaggc ccttcgacgg gttccgcagc acgagtccgc ccccggcgtt catgagccga
     6781 cgatgggtgt cggcgatcac gcgcagcccg gccgagtcca cgaagctgac ctcgccgacg
     6841 tcgagcaccg cggtggtggc gccctcgtcg agggtggcgt cgatgcgctc ctgcaggtag
     6901 ggcgacgtgt gcgggtcgag atcgccgctc agcacgaagg tggtgcggtc gccctcgtcg
     6961 acggtgacct ggaggcggtc ctgcggggtg ggggtgggtt cggtcatcgg ggctccacgg
     7021 tgtctcgtgt gcgttcaggg tggggtgggg tacagcagtc gacgccgccg ggcgcagggg
     7081 tcatggcgat cgcggccggc gcgaacctac tcgggcatcg tggggcgggg cgacggtcag
     7141 ccatccgatc ggagcagacc cttcaccttg tcggcgaccc gttcgatgtt cttca
//
LOCUS       DATISU010000319         5828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_395513, whole genome shotgun sequence.
ACCESSION   DATISU010000319 DATISU010000000
VERSION     DATISU010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395513"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VLR27_12560"
     CDS             complement(<1..302)
                     /locus_tag="VLR27_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HSP04333.1"
                     /translation="MATPSADLTTVLAEDVRPGVRLLTMNRPERLNAMDHQLVSELHD
                     RFDEVAADPDLRCVILTGAGRGFCAGLDLKGSAAVPGTEELGRPQRGLATQQHIATL"
     gene            295..1971
                     /locus_tag="VLR27_12565"
     CDS             295..1971
                     /locus_tag="VLR27_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HSP04334.1"
                     /translation="MAIGAYFHVPDPSPVAADEHAIATLQEAIAEVVPDRECIVWRDR
                     RLTWRELTERSRRVANGLLDAGVGGEVLHDPDAPGWVSPHDHIALYLTNGNEYLEAML
                     GAWKAGAAAVNVNYRYTAEELAFVLADASAVAVVYHARFAPVLADALPHVPSLRLLLV
                     VDDGTGTTPVEGSVGYEALLADASDARPALTWSPDDRYVVYTGGTTGNPKGVLWRQGD
                     FLHTALGIRGSQDELVERARRPDRPRALPAPPFMHGAAHWNALSCLLAGGTVVIQHDT
                     EHLDAADVLDTCEREHVGSLLIVGDAFARPLLDELRRGDRDLSSLRVLMTGGAVLSPT
                     IKSQMVAELPTLRIVDILGSSETGRHGLARTDAGSDSEPGVFAPSDTTVVLDETRSRQ
                     LEPGSPDRGWLAQRGRVPLGYLGDEAKTRDTFPRVDGVVHAIAGDRARRRADGAIELL
                     GRDSVTINTGGEKVHAEEVEQALKAHPAVYDALVVGRPSERWGSEIVAVVALDARADG
                     LDVDEVRAAAGEHLARYKLPKAFVVVDRVQRSASGKPDYAWARQVATGEA"
     gene            1972..2514
                     /locus_tag="VLR27_12570"
     CDS             1972..2514
                     /locus_tag="VLR27_12570"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HSP04335.1"
                     /translation="MRRAVATRPRTYLRKPSVADAEEFVARVAESEHLEPWAFPPADV
                     GAFRQWLTRGERHEIEQFLVCSREDDGIAGFVNLNGIQRGAVQRATAGWSALSPYAGR
                     GHLTDGVSMVIEVAFTQLRLHRFEADIQPGNERSRALARRCGLRLEGYSPRLVLVDGD
                     WRDHERWAVNAEDWRSARRG"
     gene            2607..3506
                     /locus_tag="VLR27_12575"
     CDS             2607..3506
                     /locus_tag="VLR27_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04336.1"
                     /translation="MIAPPNTRRLTLTRPLDLRLTLAPTRYGKGDPSCLLRSDECYRT
                     TRTPTGPATVHLVVRNDGLEAEAWGPGADWALDQLPLLVGEQDDLDGFDPGTGVVAEL
                     VRRHPGLRIGASHRVMEALVPAVCAHRVSGFEGKRAHRQVMQAYGEPAPGPSGLDLTV
                     PPDPRVLASASHHDLHPLGLERARADVIRRAAARSVTVESVVDQSPEEAEHRLRSIAG
                     VGAWTASVVRQVALGDADAVPMGDERSKHLVAWAFLGERRGTDAQMLELLEPYRGHRG
                     RVLRLLRAERPSPHRAGDEHRSA"
     gene            3591..3809
                     /locus_tag="VLR27_12580"
     CDS             3591..3809
                     /locus_tag="VLR27_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04337.1"
                     /translation="MTSYEVTLRDKTTELVVGADAYQQEGPMTTFFHLGDGRDVVDCW
                     STRVASVRTADILIVRRADEAAVLRAVG"
     gene            complement(3792..5348)
                     /locus_tag="VLR27_12585"
     CDS             complement(3792..5348)
                     /locus_tag="VLR27_12585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSP04338.1"
                     /translation="MHGPRRNTPAADLTRADLLAVVVLVAATVALRFLTRSPLWLDEA
                     LSVNIASLPLGEIGDALRRDGHPPLYYWMLHGWMELFGTGDAAVRSLSGVFGVLSLPL
                     AYEAGRRRAGHAAGLCVLLVTAITPWGVRYATEARMYSLVLMLVFAAWLLADDLLHRP
                     SRWQWAALAVVTGGALLSHYWTAYVGVAAVVVLALRWRRGDADERAGARRVASGLAAG
                     ALLFLPWVPAFIDQITSTGTPWASAGRPTAAVVELLGALGGGARYAEAVLFGAVFYVL
                     VLLGLTMLPDDRRRAVLDFAGVAGVRSEVAVGALAAAVGLVAGLITEAAFQARYAAAF
                     LPMLLIAAGVGLARLPEPWPRRLAGAALALLAGVGLVANVVDERTQGEQVATAIAADA
                     GPDDVVVICPDQLGPATLRSLPDDIAALGLPALDRPTRIDWRDYAERNEAAEPGDAVA
                     SVLERAGDGAVWMVLMPGYRTYDGYCEAVLSGLGGARPMSERVIDERAQEVFEPMILY
                     RFPAASADGS"
     gene            5407..>5828
                     /locus_tag="VLR27_12590"
     CDS             5407..>5828
                     /locus_tag="VLR27_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSP04339.1"
                     /translation="MTNTPTETSSTPGDVVVIGAGPAGLTAAYELGKRGGTATVLESD
                     DVVGGISRTVVADGYRFDIGGHRFFTKVAEVEALWHEILPDEEFMQRPRKSRIYYQGK
                     FYDYPINVVNALKNLGPVEAVRCGLSFLWVRVRPPKDLT"
BASE COUNT          822 a         2248 c         2059 g          699 t
ORIGIN      
        1 agggtggcga tgtgctgctg ggtggcgagg ccgcgctggg gccggcccag ctcctcggtg
       61 cccggcaccg ccgcgctgcc cttcaggtcg aggccggcgc agaagccacg acccgcgccg
      121 gtgaggatga cgcagcgcag atcggggtcg gcggcgacct cgtcgaagcg gtcgtgcagc
      181 tcggacacca gctggtgatc catggcgttg agccgctctg ggcggttcat cgtcagcagg
      241 cggacaccgg ggcggacgtc ttccgcgagc acggtggtca ggtcggcgga aggggtggcc
      301 atcggtgcat acttccatgt gcccgacccc agccccgtcg ccgccgacga gcacgccatc
      361 gccaccctgc aggaggccat cgccgaggtc gtgcccgacc gcgagtgcat cgtgtggcga
      421 gatcgccggc tcacgtggcg cgagctcacc gagcggtccc gacgcgtggc caacggcctg
      481 ctcgacgccg gtgtgggcgg cgaggtcctg cacgacccgg acgcgccggg ctgggtcagc
      541 ccgcacgacc acatcgccct gtacctcacc aacggcaacg agtacctcga ggcgatgctc
      601 ggtgcgtgga aggccggtgc cgcggccgtc aacgtcaact accgctacac cgccgaggag
      661 ctcgccttcg tgctggccga cgcgagcgcg gtcgccgtcg tgtaccacgc ccgcttcgca
      721 ccggtgctgg ccgacgcgct cccccacgtg ccgtcgctgc gcctgctcct cgtcgtcgat
      781 gacggcaccg gcaccacccc cgtcgagggt tcggtcgggt acgaggcgct cctcgccgac
      841 gccagcgacg cccggccggc cctgacctgg tcccccgacg accgctacgt cgtctacacg
      901 ggcggcacca ccggcaaccc caagggcgtg ctgtggcgcc agggcgactt cctccacacc
      961 gccctcggga tccgcgggtc ccaggacgag ctggtcgagc gggcccggcg cccggaccgg
     1021 ccacgtgcgc tccccgctcc cccgttcatg cacggcgcgg cccactggaa cgccctgtcc
     1081 tgcctgctgg ccgggggcac cgtcgtgatc cagcacgaca ccgagcacct cgacgccgcc
     1141 gacgtcctcg acacctgcga gcgggagcac gtcggctccc tgctcatcgt gggcgacgcg
     1201 ttcgcccgac ccctgctcga cgagctccgc cggggcgacc gcgacctgtc gtcgctgcgg
     1261 gtgctcatga ccggcggggc ggtcctgtca ccgacgatca agtcccagat ggtcgccgag
     1321 ctgcccacgc tgcgcatcgt cgacatcctc gggtcgtccg agaccggccg ccacgggctg
     1381 gcccgcaccg acgccggctc cgattccgag cccggcgtct tcgcaccgtc cgacaccacc
     1441 gtggtgctcg acgagacccg ctcccgccag ctcgagcccg gctcgcccga ccgcggctgg
     1501 ctggcccagc gcggccgcgt gccgctcggc tacctcggcg acgaggccaa gacccgtgac
     1561 acgttcccgc gggtcgacgg cgtcgtccat gccatcgccg gcgaccgggc acgacggcgg
     1621 gccgacggcg cgatcgagct gctcggtcgg gactcggtca ccatcaacac cggcggggag
     1681 aaggtccacg ccgaggaggt cgagcaggcc ctgaaggcgc acccggcggt gtacgacgcg
     1741 ctggtcgtgg ggcgccccag cgagcggtgg ggctccgaga tcgtggcagt tgtcgccctc
     1801 gacgcacgag cggatggcct cgatgtcgac gaggtccggg ccgcggccgg cgagcacctg
     1861 gcccggtaca agctgcccaa ggccttcgtc gtggtcgacc gcgtgcagcg cagcgccagc
     1921 ggcaagcccg actacgcctg ggcccgccag gtcgccaccg gggaggcctg agtgcgacga
     1981 gccgtggcca cccggccgcg cacgtacctg cgcaagccct ccgtggccga cgccgaggag
     2041 ttcgtggccc gcgtcgccga gagcgaacac ctcgagccgt gggcgttccc acccgccgac
     2101 gtcggcgcgt tccgccagtg gctcacccgg ggcgagcgcc acgagatcga gcagttcctg
     2161 gtgtgctccc gcgaggacga cggcatcgcc gggttcgtca acctcaacgg catccagcgc
     2221 ggtgccgtgc agcgcgccac cgccggctgg tcggcgctgt cgccctacgc cggccgtggc
     2281 cacctcaccg acggcgtctc gatggtgatc gaggtcgcct tcacgcagct gcgcctgcac
     2341 cggttcgagg ccgacatcca gccgggcaac gagcgctcgc gcgccctcgc ccgccgctgc
     2401 gggctccgcc tggagggcta ctccccccgc ctcgtgctcg tcgacggtga ctggcgcgac
     2461 cacgagcggt gggccgtgaa cgccgaggac tggcggtccg cacggcgcgg gtgagacccg
     2521 gtccggattg ctccggtttg gcagcgccgg acggcgaagc ggagaatgtc acacccccgg
     2581 agcggcctcc ggagggagcc cgctcgatga tcgccccgcc caacacgcgc cggttgacgc
     2641 tgacccgtcc gctcgacctg cgcctcacgc tcgccccgac ccgctacggg aagggcgatc
     2701 cgtcgtgcct gctccgatcc gacgagtgct accgcaccac ccggacgccc accggtccgg
     2761 ccaccgtgca cctggtcgtc cgcaacgacg gcctggaggc cgaggcctgg gggccggggg
     2821 ccgactgggc cctcgaccag ctgcccctgc tcgtcggtga gcaggacgac ctcgacggct
     2881 tcgaccccgg caccggcgtc gtggccgagc tggtccggcg ccacccgggg ctgcgcatcg
     2941 gcgcatcgca ccgggtcatg gaggccctgg tgccggccgt gtgcgcccac cgggtcagcg
     3001 gcttcgaggg caagcgggcc caccgccagg tcatgcaggc ctacggcgaa cccgccccgg
     3061 gcccgtccgg cctcgacctg acggtgccgc ccgacccccg ggtcctggcc agcgccagcc
     3121 accacgacct gcacccgctg gggctcgagc gggcccgcgc cgacgtcatc cgccgcgccg
     3181 ccgcccggtc cgtcaccgtg gagtcggtgg tggaccagtc ccccgaggag gccgagcacc
     3241 gcctgcgctc catcgccggc gtcggcgcgt ggaccgcctc cgtcgtgcgc caggtcgcgc
     3301 tcggcgatgc cgacgccgtg ccgatgggcg acgagcgcag caagcacctc gtggcctggg
     3361 cgttcctcgg cgagcgccgg ggcacggacg cccagatgct ggagctgctc gagccctacc
     3421 ggggccaccg cggccgggtg ctgcgcctcc tgcgggccga gcggcccagc ccccaccggg
     3481 ccggggacga gcaccgcagc gcctgagggc gccgaccaca ggccctgcac aggcaagtca
     3541 cagggttgtc cctcttcctc cgcacagggc acccctcgta cggtcagccc atgacgagct
     3601 acgaggtgac cttgcgggac aagacgacag agctcgtggt cggggccgac gcctaccagc
     3661 aggagggtcc gatgacgacg ttcttccacc ttggcgacgg gcgcgacgtg gtcgactgct
     3721 ggtcgacccg cgtggccagc gtgcgcaccg ccgacatcct catcgtccgg cgggccgacg
     3781 aggccgcggt gctacgagcc gtcggctgag gcggccggga accggtagag gatcatcggc
     3841 tcgaacacct cctgggcgcg ctcgtcgatc acgcgctcgc tcatcgggcg agcaccaccg
     3901 aggccgctca gcaccgcctc gcagtagccg tcgtaggtcc ggtagccggg catgagcacc
     3961 atccacaccg cgccgtcgcc ggcccgctcc agcaccgagg cgacggcgtc gcccggctcg
     4021 gccgcctcgt tccgctcggc gtagtcgcgc cagtcgatgc gcgtcggtcg gtccaacgcc
     4081 ggcaggccga gggcggcgat gtcgtccggc agcgagcgca gggtggcagg gccgagctgg
     4141 tccgggcaga tgacgacgac gtcgtcgggt ccggcgtccg ccgcgatcgc cgtcgcgacc
     4201 tgctcgccct gggtccgctc gtcgaccacg ttggcgacca gacccacgcc ggccagcagc
     4261 gcgagcgccg cccctgccag gcgccggggc cacggttccg gcaaccgggc cagcccgacg
     4321 ccggccgcga tgagcagcat cgggaggaac gccgcggcgt agcgggcctg gaacgcggcc
     4381 tcggtgatca ggccggccac caggcccacc gcggcggcga gcgcaccgac cgccacctcg
     4441 ctgcgcacgc ccgcgacgcc cgcgaagtcg agcaccgccc gccggcggtc gtccgggagc
     4501 atggtgagcc cgagcaggac cagcacgtag aacacggcgc cgaacagcac ggcctcggcg
     4561 taccgggcac cgccgccgag cgcgccgagc agctcgacga cggcggcggt gggccgaccg
     4621 gccgaggccc acggggtccc ggtgctggtg atctggtcga tgaacgccgg cacccacggg
     4681 aggaacagca gcgcaccagc ggcgaggccg gacgcgaccc ggcgcgcacc cgctcgctcg
     4741 tcggcgtcgc cgcgccgcca gcgcagcgcc aggaccacca cggcggcgac gccgacgtac
     4801 gcggtccagt agtggctgag cagcgcgcca ccggtcacca cagcgagcgc ggcccactgc
     4861 caccgactcg gccggtggag caggtcgtcg gccagcagcc acgcggcgaa cacgagcatc
     4921 aggaccagcg agtacatgcg ggcctcggtg gcgtaccgca cgccccacgg ggtgatggcg
     4981 gtgaccagca gcacgcacaa gccggcggcg tggccggcgc gccgccgacc ggcctcgtag
     5041 gccaggggca gcgagagcac cccgaacacg ccggagagcg agcgcacggc cgcatcgccg
     5101 gtgccgaaca gctccatcca gccatggagc atccagtagt agagcggggg gtgcccgtcg
     5161 cggcgcagcg cgtcgccgat ctcccccagg ggcagcgacg cgatgttgac gctgagcgcc
     5221 tcgtccagcc agagcgggga gcgggtcagg aaccgcagcg ccaccgtcgc cgccacgagc
     5281 acgacgaccg cgagcaggtc ggcccgggtc aggtcagcgg cgggcgtgtt ccgtcgggga
     5341 ccgtgcaccg gcgtacgcta ggcggctgct ctccctgccg ccgaacgacg agatcgcatc
     5401 agcaccgtga ccaacacccc caccgaaacc tcgtccacgc ccggagacgt cgtcgtcatc
     5461 ggcgccggac ccgccgggct gaccgccgcc tacgagctgg gcaagcgtgg tggcaccgcc
     5521 accgtgctcg agtccgacga cgtcgtcggc ggcatcagcc gcacggtcgt cgccgacggc
     5581 taccgcttcg acatcggcgg ccaccgcttc ttcaccaagg tggccgaggt cgaggccctg
     5641 tggcacgaga tcctccccga cgaggagttc atgcagcggc cccgcaagag ccgcatctac
     5701 taccagggca agttctacga ctacccgatc aacgtcgtga acgccctgaa gaacctcggc
     5761 ccggtcgaag ccgtccgctg cgggctgtcg ttcctctggg tccgggtccg tccgccgaag
     5821 gacctcac
//
LOCUS       DATISU010000320         3673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_534935, whole genome shotgun sequence.
ACCESSION   DATISU010000320 DATISU010000000
VERSION     DATISU010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534935"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VLR27_12595"
     CDS             complement(<1..552)
                     /locus_tag="VLR27_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HSP04340.1"
                     /translation="MPDTATTLTIDRGVATITLDRPDARNALSADLVDSLADHLDAAV
                     ADDAARVVVLTNAGTTFCAGADLKSGTSRSPRRSLVDVLTTMLDSPKPVIGRIAGHCM
                     GGGVGLAAACDISVITDDAKVGFTEVRIGVAPAMISVVCLPKMRRADAAELFLSGERI
                     TAARAVEVGLLNRVVPRDDLDDAV"
     gene            complement(575..1051)
                     /locus_tag="VLR27_12600"
     CDS             complement(575..1051)
                     /locus_tag="VLR27_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04341.1"
                     /translation="MASDSVERERTWLVRSLPHPLPPGVPVVQGYLVADDGVAVRVRR
                     SCTRQVIAIQACGAGARTELGWDLTPAQFDAVWPLTRRARVEKVRHEVRIGSRTATVD
                     VFAGPLAGLALVSVALDDDPFAAPDWFGEEVSGEPRFAEAVLARDGLPAGHRAPTD"
     gene            1278..1649
                     /gene="rpsL"
                     /locus_tag="VLR27_12605"
     CDS             1278..1649
                     /gene="rpsL"
                     /locus_tag="VLR27_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HSP04342.1"
                     /translation="MPTIQQLVRKGRQSKMTKAKTPALKGAPQRRGVCTRVFTVTPKK
                     PNSALRKVARVRLTSGIEVTAYIPGEGHNLQEHSIVLVRGGRVKDVPGVRYKIIRGTL
                     DTSGVRDRKQARSRYGAKKDA"
     gene            1651..2121
                     /gene="rpsG"
                     /locus_tag="VLR27_12610"
     CDS             1651..2121
                     /gene="rpsG"
                     /locus_tag="VLR27_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HSP04343.1"
                     /translation="MPRKGPAPRREIKPDPIYDSTVVTQLTNKILLSGKRSTAERIMY
                     DAMSIVESKTGSEPLSTLKRALDNIKPQLEVKSRRVGGATYQVPIEVRPRRANTLAIR
                     WIITFARARRERTMAERLANEILDASNGLGASVKRREDMHKMAESNKAFAHYRW"
     gene            2223..>3673
                     /gene="fusA"
                     /locus_tag="VLR27_12615"
     CDS             2223..>3673
                     /gene="fusA"
                     /locus_tag="VLR27_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HSP04344.1"
                     /translation="MTEKRTNSIAKTRNIGIMAHIDAGKTTTTERILFYTGRNYKIGE
                     VHEGGATMDWMEQEQERGITITSAATTCQWKDHTINIIDTPGHVDFTVEVERSLRVLD
                     GAVAVFDGVAGVEPQTETVWGQANKYGVPRMCFINKMDRLGANFYDALDSIKDRLNAN
                     TAVLQLPIGYEAEYAGVVDLISMKALIWESEDLGASWTEGEIPDDLKDKAAEYRAELI
                     ETVATADETLMMKFLEDEEITEDELHEAIRKATIAGDIVPVLNGTAFKNKGVQPLLDA
                     VIRYLPSPADLPPVNGLNVKETEKLTRELTDEAPFSALAFKIMTDPHVGKLTYFRVYS
                     GTLAKGDTVLNVRSGNKERLGRLLQMHANTREDRERIFSGEIIAGIGMKNTKTGDTLC
                     DPDNPIVLESLEFPEPVIHVAVEPKTKADQEKMGKALQSLSEEDPTFTVRTDEETGQV
                     IIAGMGELHLEVLVDRMLREFRVDATVGKPQVA"
BASE COUNT          641 a         1343 c         1208 g          481 t
ORIGIN      
        1 cacggcgtcg tcgaggtcgt cgcggggcac gacgcggttg agcagcccga cctcgacggc
       61 tcgggcggcg gtgatccgct cgccggacag gaacagctcg gcggcgtcgg cccggcgcat
      121 cttgggcagg cacaccaccg agatcatcgc cggggccacc ccgatgcgga cctcggtgaa
      181 gccgaccttg gcgtcgtcgg tgatcacgga gatgtcgcac gccgcggcga ggccgacgcc
      241 accgcccatg cagtgcccgg cgatccgccc gatcaccggc ttggggctgt cgagcatggt
      301 ggtcaggacg tcgacgagcg accggcgggg cgagcgcgac gtcccggact tcaggtcggc
      361 gccggcgcag aacgtggtgc ccgcgttggt gaggacgacg acccgggcgg cgtcgtcggc
      421 gacggcggcg tcgaggtggt cggccaggct gtcgacgaga tcggcggaca gcgcgttgcg
      481 ggcgtcgggc cggtcgaggg tgatcgtggc caccccccgg tcgatcgtga gcgtcgtcgc
      541 ggtgtcgggc atgtcgggcc tctcgtcggt ggggtcagtc ggtgggggcg cggtgcccgg
      601 ctggcaggcc gtcccgggcg agcacggcct cggcgaagcg gggctcgccg gagacctcct
      661 cgccgaacca gtccggggcg gcgaaggggt cgtcgtcgag cgcgaccgag acgagcgcca
      721 ggccggccag cggtccggcg aacacgtcga cggtggcggt ccgcgatccg atgcggacct
      781 cgtggcgcac cttctccacg cgggcccggc gcgtgagcgg ccacacagcg tcgaactgcg
      841 ccggcgtgag gtcccagccg agctcggtcc gggccccggc gccgcacgcc tggatggcga
      901 tgacctgtcg cgtgcaggag cgtcggaccc gcacggcgac gccgtcgtcg gcgaccaggt
      961 agccctgcac gacggggacg cccggcggca gcggatgggg gagcgagcgc acgagccagg
     1021 tccgctcgcg ctccacgctg tcggacgcca tggcgagcga tcctaggagt ggacatccgc
     1081 cgcgcccact cgcccggctg cgaggatggg caccggcacc cccgctgtcg ccgggttgtg
     1141 tgccgccgag cctcgcccat agtctgtcct gtcggtcatg cgcgcccccg gtgcgaacct
     1201 cacgaggtgc gccagaccgt gcgatcgccc cgatacccaa gcacacacgc tttcgatccg
     1261 tcaggcagag gacacccgtg cccacgattc agcagctggt ccgaaagggc cggcagtcca
     1321 agatgaccaa ggcgaagacg ccggccctca agggtgcgcc ccagcggcgc ggcgtctgca
     1381 cccgggtgtt caccgtcacg cccaagaagc cgaactcggc gctgcgcaag gtcgcccgtg
     1441 tccgtctcac ctcgggcatc gaggtcaccg cgtacatccc cggcgagggc cacaacctcc
     1501 aggagcactc catcgtgctc gtgcgtggtg gccgtgtgaa ggacgtcccg ggcgtccgct
     1561 acaagatcat ccgcggcacg ctcgacacgt cgggcgtccg cgaccgcaag caggcccgta
     1621 gccgctacgg cgccaagaag gacgcctgag atgcctcgca agggacccgc accacgtcgt
     1681 gagatcaagc ccgatccgat ctacgactcc accgtcgtca cccagctgac caacaagatc
     1741 ctgctctcgg gcaagcgctc gaccgccgag cggatcatgt acgacgccat gtcgatcgtc
     1801 gagtccaaga cgggctccga gccgctctcc acgctgaagc gcgccctcga caacatcaag
     1861 ccccagctcg aggtcaagag ccgtcgcgtc ggtggtgcca cctaccaggt gccgatcgag
     1921 gtccgccccc gccgggcgaa caccctcgcc atccgctgga tcatcacctt cgcccgcgcc
     1981 cgccgtgagc gcaccatggc cgagcgcctc gccaacgaga tcctcgacgc cagcaacggc
     2041 ctcggcgcct ccgtcaagcg gcgtgaggac atgcacaaga tggccgagtc caacaaggcc
     2101 ttcgcgcact accgctggta gtgcccgccc tccccctgat ctgtccatcc cacgcgggcc
     2161 atcggcccca cgaccgggcc gcctgtcacc aggccccggt cgtctttctg tgagagccct
     2221 ccatgactga gaagcgcacc aactccatcg ccaagacccg caacatcggg atcatggcgc
     2281 acatcgacgc cggcaagacc accacgaccg agcgcatcct cttctacacc ggccgcaact
     2341 acaagatcgg cgaggtccac gagggcggcg ccacgatgga ctggatggag caggagcagg
     2401 agcgggggat caccatcacc tccgcggcca ccacctgcca gtggaaggac cacaccatca
     2461 acatcatcga cacgcccggc cacgtcgact tcacggtcga ggtcgagcgg tcgctgcgcg
     2521 tgctcgacgg tgccgtcgcc gtgttcgacg gtgtcgccgg tgtcgagccc cagaccgaga
     2581 cggtgtgggg ccaggccaac aagtacggcg tcccccgcat gtgcttcatc aacaagatgg
     2641 accgcctcgg cgccaacttc tacgacgcgc tcgactccat caaggaccgg ctgaacgcca
     2701 acacggccgt gctgcagctg cccatcggct acgaggccga gtacgccggc gtggtcgacc
     2761 tcatcagcat gaaggccctg atctgggaga gcgaggacct cggcgccagc tggaccgagg
     2821 gcgagatccc cgacgacctc aaggacaagg ccgccgagta ccgcgccgag ctcatcgaga
     2881 ccgtcgccac cgccgacgag accctcatga tgaagttcct cgaggacgag gagatcaccg
     2941 aggacgagct ccacgaggcc atccgcaagg cgaccatcgc cggcgacatc gtgcccgtgc
     3001 tcaacggcac cgccttcaag aacaagggcg tccagccctt gctcgacgcg gtcatccgct
     3061 acctgccgag cccggccgac ctgcccccgg tcaacggcct gaacgtcaag gagaccgaga
     3121 agctcacccg tgagctgacc gacgaggccc cgttctcggc cctggccttc aagatcatga
     3181 ccgaccccca cgtcggcaag ctcacctact tccgggtgta ctccggcacg ctcgccaagg
     3241 gcgacaccgt gctcaacgtg cgctccggca acaaggagcg cctcggccgc ctgctgcaga
     3301 tgcacgccaa cacccgcgag gaccgcgagc gcatcttctc cggcgagatc atcgccggca
     3361 tcggcatgaa gaacaccaag accggtgaca cgctctgcga tccggacaac ccgatcgtgc
     3421 tcgagtccct cgagttcccc gagcccgtga tccacgtggc cgtggagccc aagaccaagg
     3481 ccgaccagga gaagatgggc aaggccctcc agtcgctgtc ggaggaggac cccaccttca
     3541 ccgtgcgcac cgacgaggag accggccagg tcatcatcgc cggcatgggc gagctgcacc
     3601 tcgaggtgct ggtcgaccgc atgctccgcg agttccgcgt cgacgccacc gtgggcaagc
     3661 cccaggtcgc cta
//
LOCUS       DATISU010000321         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_442630, whole genome shotgun sequence.
ACCESSION   DATISU010000321 DATISU010000000
VERSION     DATISU010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442630"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VLR27_12620"
     CDS             <1..931
                     /locus_tag="VLR27_12620"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HSP04345.1"
                     /translation="MVGMASDAARILVLPAGGRPWLADAVRAGGGEPVTDGPAGAVVW
                     TDPKDAAGLRRLLDERPDIDWVQLPWAGIEPYVEVLDHDRRWTCGKGVYADPVAEQAL
                     MLALAGLRGMGTYARATTWEGPIGHNLLGARVVVVGGGGISESLVRLLQPFRCEIVVV
                     RRTEQPFPGATRTVTLDRLDDELPAADLVVLAVALTPETEGLFDGRRLRKLASHAWLV
                     NVARGRHVVTDDLVEVLQEGAIGGAGLDVVDPEPLPDGHPLWTLPNCFITPHVGNTPE
                     MAVPLLSARVQENVRRYLAGEELLGPVDVDAGY"
     gene            complement(925..1275)
                     /locus_tag="VLR27_12625"
     CDS             complement(925..1275)
                     /locus_tag="VLR27_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04346.1"
                     /translation="MAEDASNPLAQVLGRLGLSGDQLSSLQQQLGRVMPSEQMKLMRD
                     LVDTFSPSGDQLSAIRRQLEAQRVQVEAMLAQLEDIESTVDRLATTAEQLRAMQEPFQ
                     RLTHLFSPGQDRGQ"
     gene            1306..1677
                     /locus_tag="VLR27_12630"
     CDS             1306..1677
                     /locus_tag="VLR27_12630"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP04347.1"
                     /translation="MPVIGFDHLVLNVADARRSVRFYVDVLGLAPERVDEWEAGEAPF
                     PSVRVDATTVIDIMELDRGGENVDHLCLVVDDDIDALAAREDLHVLVGPVDRWGAQGV
                     ARSVYLRDPDGNLVELRRYPR"
     gene            1701..1774
                     /locus_tag="VLR27_12635"
     tRNA            1701..1774
                     /locus_tag="VLR27_12635"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1735..1737,aa:Val,seq:cac)
     gene            1936..2382
                     /locus_tag="VLR27_12640"
     CDS             1936..2382
                     /locus_tag="VLR27_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04348.1"
                     /translation="MEADAQVVGDLGEPFAYVACGSEPESTPVDEAGGAHQLRQLAGA
                     RGVDPQRLGKLSGLVVGVVTTEDRVGFDIGVQRGPKVVHEVAGREAQQDLAAFRGDAR
                     TAGAPTASTFLEQILTSGHAIHRASERRGSDRGTIVHPSEPSSRRA"
     gene            complement(2349..2591)
                     /locus_tag="VLR27_12645"
     CDS             complement(2349..2591)
                     /locus_tag="VLR27_12645"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HSP04349.1"
                     /translation="MSARFKVRVERAGVPMLRLHDLRHTAASIMLASGEQARVVQERL
                     GHSTVAFTLARYQKVLPGAQAEAATRYARLLDGSDG"
     gene            2606..>2867
                     /locus_tag="VLR27_12650"
     CDS             2606..>2867
                     /locus_tag="VLR27_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04350.1"
                     /translation="MANRRLREDEAELVGVIGLSERQLVLGLFDAVAVQGIDGGRIEV
                     DHPAGAVRLHREHRRSGAHHRELLGDGDPPCVEVDVGPAQSER"
BASE COUNT          396 a          983 c         1043 g          445 t
ORIGIN      
        1 gatggtgggc atggcttccg acgctgcccg catcctcgtc ctgcccgccg gcggccgccc
       61 gtggctggcc gatgctgtcc gtgccggggg cggtgagccg gtcaccgacg ggcctgccgg
      121 cgccgtggtc tggaccgatc cgaaggacgc ggccgggttg cgccggctgc tggacgagcg
      181 gcccgacatc gactgggtgc agcttccgtg ggcgggcatc gagccctacg tcgaggtgct
      241 cgaccacgac cggcgctgga cgtgcggcaa gggcgtctac gccgaccccg tcgccgagca
      301 ggcgctgatg ctcgccctcg ccgggctgcg gggcatgggt acctacgccc gggccacgac
      361 gtgggagggg ccgatcgggc acaacctgct cggcgcccgc gtggtcgtcg tcggtggcgg
      421 cggcatcagc gagtccctcg tgcgcctgct ccagccgttc cgctgcgaga tcgtggtcgt
      481 gcggcgcact gagcagccgt tcccgggagc gacccgcacg gtcaccctcg accggctgga
      541 cgacgagctg ccggctgccg acctggtcgt gctggcggtc gccctcacgc cggagaccga
      601 gggcctcttc gacggtcgtc gcctccggaa gctggcgtcc catgcctggc tcgtgaacgt
      661 ggctcgcggt cgccacgtgg tcaccgatga cctggtcgag gtactgcagg agggcgccat
      721 cgggggtgca ggcctcgacg tggtcgaccc cgagccgctt cccgacgggc acccgctgtg
      781 gacgttgccc aactgcttca tcacccccca cgtcgggaac acgccggaga tggccgtgcc
      841 gctgctgtcg gcgcgggtcc aggagaacgt gcggcggtac ctggcggggg aggagctcct
      901 cggccccgtc gacgtcgacg ccggctactg accccggtcc tggcccgggg agaacaggtg
      961 ggtcaggcgc tggaacggct cctgcatggc acggagctgc tcggcggtgg tggccaggcg
     1021 atcgacggtg gactcgatgt cctcgagctg cgccagcatc gcctcgacct ggacccgttg
     1081 ggcctccagc tggcggcgga tggcgctgag ctggtccccg gacggggaga acgtgtcgac
     1141 caggtcccgc atgagcttca tctgctcgct gggcatcacc cgcccgagct gctgctggag
     1201 cgaggagagc tggtccccgg acaggcccag gcgaccgagc acctgggcga gcgggttgga
     1261 ggcgtcttca gccatggatc gaccgtaccg tggcacgatg gggccgtgcc cgtgatcggc
     1321 ttcgaccacc tcgtgctcaa cgtcgccgac gcccgccgct ccgtccgctt ctacgtggac
     1381 gtgctcggcc tggcccccga acgggtggac gagtgggagg ccggcgaggc cccgttcccg
     1441 agcgtgcggg tcgacgccac cacggtcatc gacatcatgg agctcgaccg tggcggcgag
     1501 aacgtcgacc acctctgcct ggtggtcgac gacgacatcg acgccctcgc cgcccgcgag
     1561 gacctgcacg tcctggtcgg accggtcgac cgctgggggg cccagggcgt cgcccgatcc
     1621 gtctacctgc gagaccccga cggcaacctg gtcgagctcc gtcgctaccc gcgctgagtg
     1681 ctacagtcgc ccgctcctac gggcgtatag ctcagctggc tagagcgcac gcttcacacg
     1741 cgtgaggtca caggttcaag tcctgttacg cccactcctg tttgcccagg tcacaggctt
     1801 gtcgggcccc gaaatggggc cggttggtca cgaaatggtc acgaacggat cagcaccggc
     1861 cggcatctga gggcccgaac cagcacgatc cggcattgcc ggggctgcgt tcagcgcccg
     1921 ccgtcgagct cgcacgtgga ggctgatgcc caggtcgtcg gcgatctcgg cgagccgttt
     1981 gcctacgttg cgtgcggttc cgaaccagag tcgacgcccg tggacgaggc cggcggcgcc
     2041 caccagctgc gacagctggc cggtgccagg ggagtggatc cgcagcgact cggaaaactc
     2101 tcaggcttgg tcgtcggtgt cgtgaccacc gaggaccggg tcggcttcga catcggcgtc
     2161 cagcgcggcc cgaaagtcgt ccatgaggtc gccggtcgtg aggcgcagca agatctcgcg
     2221 gccttccggg gtgatgcgcg gaccgccggc gcgccgacgg cgtcgacgtt cctcgagcag
     2281 atcctcacca gcggtcacgc gatccatcgt gcctccgagc gtcgaggctc cgacaggggg
     2341 acgatcgttc atccgtcgga gccgtcgagc aggcgggcgt agcgggttgc cgcctcggcc
     2401 tgcgctccgg gcagcacctt ctggtagcgg gccagcgtga aggcgaccgt ggagtgcccg
     2461 aggcgctcct ggacgacacg agcttgctcc ccggaggcga gcatgatcga cgcagccgtg
     2521 tggcgcaggt cgtggagccg aagcatcggg accccggccc gttccacccg taccttgaac
     2581 cgcgctgaca cgcgcttggg gtggtgtggc gaaccgtcgg cttcgcgaag acgaggccga
     2641 gctcgtcggg gtcataggac tctccgagcg ccagcttgtg ctcggcttgt tcgatgcggt
     2701 ggcggtgcag ggcatcgatg gtggccggat cgaggtcgac cacccggcgg gagcggtcag
     2761 acttcaccgc gagcatcgtc ggagcggcgc ccaccatcgt gaactgctgg gtgatggaga
     2821 tccgccctgc gtcgaggtcg acgtcggacc agcgcagtcc gagcgcc
//
LOCUS       DATISU010000322         8196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_162844, whole genome shotgun sequence.
ACCESSION   DATISU010000322 DATISU010000000
VERSION     DATISU010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8196
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162844"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VLR27_12655"
     CDS             <1..1037
                     /locus_tag="VLR27_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HSP04351.1"
                     /translation="ERSSEVSDTTIPETSRKDWMSDQEVRWCPGCGDYSILAAVQLAL
                     PDRGVRREEVVFVSGIGCAARFPYYMNTYGMHSIHGRAPAIATGLAMARPDLHVFVVS
                     GDGDALSIGGNHLIHALRRNVNLTILLFNNQIYGLTKGQYSPTSETGKVTKSTPFGSI
                     EKPFNPVSVALGAEASFVARTHDMDRNHMMETIKRAHDHQGASIVEIYQNCNVFNDGA
                     FGQITAKGARDEMLIDLRHGEPILFGADRQRGVVLDGGGVRLAEVAEVGMDAVLVHDE
                     TRPDPSIAFMLSRIATGPHEPTPVGVFRAVARDEYGSRVNQQLAAAQEQRGPGNLHDL
                     LRSGATWEVE"
     gene            complement(1038..1739)
                     /locus_tag="VLR27_12660"
     CDS             complement(1038..1739)
                     /locus_tag="VLR27_12660"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HSP04352.1"
                     /translation="MQVTQLWRYPVKSMIGEALTAVDVGALGIDGDRQWGLVDRTTGL
                     VLTARRVPQLLFAEPVARDDRMAVRLPDGSVTDDDDVLSAWVGRPVELRRPAPDEHGH
                     YAVEGAGLDDEAPEQWEGPAGVWHDSKRTRLSIVAEEALGARDVRRFRPNVVIRGGDE
                     RDLVGSRIRMGSVVADVVKEIDRCVIVTRPQPGLDRDTSVLTQVHAERAGNLGVGALV
                     VAAGRISLGDALEIE"
     gene            1776..2705
                     /locus_tag="VLR27_12665"
     CDS             1776..2705
                     /locus_tag="VLR27_12665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HSP04353.1"
                     /translation="MDLRIFTEPQQGASYDTLLAAARATEAAGFSAFFRSDHYLKMGD
                     ASGLPGPTDAWITLAGLARETESIRLGTLVTAATFRLPGPLAISVAQVDQMSGGRVDL
                     GIGTGWYDDEHTAYGIPFPPLKERFEILEEQLEILTGLWRTPVGETFGFDGRHHTVSG
                     SPALPKPAQDGGPPIIIGGGGPKKTPSLAARFASEFNLPFAPKDVWVTQRGRVQDACR
                     DIDRDPDELTYSVALVVCVGEDEAEIARRAASIGREVDELRENGVCGTPAEAVARLQE
                     WGEAGAERVYLQVLDLDDLDHIELLGREVRTAV"
     gene            complement(2709..3920)
                     /locus_tag="VLR27_12670"
     CDS             complement(2709..3920)
                     /locus_tag="VLR27_12670"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP04354.1"
                     /translation="MTFDRWRGTELSAALAAGGLAALLALGGSTVGGALRVALVAAIV
                     TGLLLGLRGRVRRAAALAAGLGALALAIGVGFLPHLLDSPGGVRGYVAVGAVLGGLGL
                     VGFGTASATAGLRRRGRIPTWLGMVVLVLLTLVVVGPAVAVVNVPDSSSGRTAAEPPL
                     DAEPVSLPTSDGVTLAAWWVPGTNGAAVLVRHGAGSTHEGALAQATVLVEHGYGVLLM
                     DARGHGESTGRAMDFGWWGDADIAAGLDHVTTRPDVDPDRIGLLGLSMGGEEAIGAAT
                     DHRVRVVVAEGATARRADDKDWLSDVHGWRGAVQERIEQLQYGLTDLLTEAPRPTVLR
                     AAVARADVPVLLIAAGQVEDERHAAAWIAAGAPERVVVWTVDGAGHTDGIGVAPDEWE
                     RRVVGFLDENL"
     gene            4062..5444
                     /locus_tag="VLR27_12675"
     CDS             4062..5444
                     /locus_tag="VLR27_12675"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HSP04355.1"
                     /translation="MERRTAFLLVVALVAAAACDDGSRTTSAPTTQRSPVVVDTDGGS
                     DDAIALLYLLQHPDVDVIGITVSGTGIVHCDPGVANVLGWVELAAAGDVPVSCGRAEP
                     VAGERSFPEAWRTQADGRYDGILPVGRGAADPRPAARLMRDLVTDEGGLVTVLALGPL
                     TNVADALESTPEMAEAIGSLVVMGGAFDVPGNVDLPEAPAAAATEWNLYVDPAAAQAV
                     LDADIEVTFVPLDSAVGIDAFDVRALARAASSPAGRAAADLLLSSPFFTSPGFALWDP
                     LAAAVVAEPGAFVSSERPTRIVVDGPEAGRTVADGGRPVLVARPQRAGAFVAGLLGVL
                     SGAGPPLDVDRSPDLSVAVGEGTCVASDQDLAAGPVVLDVGTRGAVAIGVMDPDRDLG
                     DVEALLASGAPAAAPEWFPLVALLQGGGPVVARVDPGQLDVVCIDVGADGALHVAGHV
                     ELTVRASDGP"
     gene            5497..7926
                     /locus_tag="VLR27_12680"
     CDS             5497..7926
                     /locus_tag="VLR27_12680"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HSP04356.1"
                     /translation="MPSTPLPAVDDLTLEEKCRLLGGASTWRTHPIERVGIPAVKMSD
                     GPNGVRGDGLGARRTPGVVIPVGIAVGATWNPALAAELGDLLGTESRRKGAHVLLAPT
                     VNLQRTPIGGRVFECLSEDPELTARLAVPLVEAVQAHDVAVTVKHYVANDTEIDRMSV
                     DVDVDDRVLRELYLRPFEATVTEADAWGIMSAYNQRGGEHCAASRELLTGILRDEWGF
                     DGFVVSDWYGSHDTVASIRAGLTVPMPGPSTIYGERLQAAVEAGDVEEALVDAQVEDL
                     LRLMARVKVTERDAEQREETVDDPAERALCRRAVIEGLVLCRNEGGTLPIASGASVAV
                     IGPNAEDTRIMGGGSASLQALPNRSLLEALTDRAGSVVHEPGVRIDRLPPPLTEAVLR
                     TPDGEPGLRVDYRDGLDPDSPIVVTDVTPESMLRFFGSTPEGVDPERFHVTVTGTFVP
                     ERTGTHLLSAVLTGAGRIEVGDIAVLDDPDRALPRGPLFFGFGSEEQEATIECEEGVA
                     VPIRITTTGRGGYAAIRLGVRAPEPPDMLERAVAAARDADVAVVVVGTNDEWETEGED
                     RTSIALPGDQDELIRRVAEANPRTVVVVNAGSPVSMPWIDDVAAVLLAYFGGNELADG
                     VTDVLLGDADPGGRLPLTYPKALEDTPAWRWYAPVDGVQHYGEGLLMGYRGFDAAGTE
                     PLFPFGHGLSYGSSEWGEASLSSWIAGPGDGIDVTVPIRATGERAATVVVQAYLSGPD
                     GFGRPPKVLAGFAKSTVEPGAEEDVVVTVPPEAFRRWDGDGWVVDEGTWEVVVAASAT
                     DVRQRLALEVG"
     gene            complement(7965..>8196)
                     /locus_tag="VLR27_12685"
     CDS             complement(7965..>8196)
                     /locus_tag="VLR27_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04357.1"
                     /translation="LEQARAALPAHDLSYQHGVANVTEAVAEGRAQAAVLMRPATVAQ
                     IQAISHGGERMPPKTTFFAPKPATGIVFRALD"
BASE COUNT         1104 a         3031 c         2984 g         1077 t
ORIGIN      
        1 cagaacggag cagcgaagtg agcgacacca ccatcccaga gaccagccgc aaggactgga
       61 tgtcggacca ggaggtccgc tggtgcccgg gctgcggtga ctacagcatc ctcgccgccg
      121 tgcagctggc gctgcccgac aggggggtgc gtcgcgagga ggtcgttttc gtctccggca
      181 tcggctgcgc cgcccgcttc ccgtactaca tgaacaccta cgggatgcac tcgatccacg
      241 gccgtgcacc ggccatcgcc accgggctgg ccatggcccg acccgacctg cacgtcttcg
      301 tcgtgtccgg tgacggcgac gccctctcca tcggcggcaa ccacctcatc catgcgctgc
      361 gccgcaacgt gaacctcacg atcctgctgt tcaacaacca gatctacggg ctcaccaagg
      421 ggcagtactc gcccacgtcg gagaccggca aggtcaccaa gtccacgccg ttcgggtcca
      481 tcgagaagcc gttcaacccg gtgtcggtcg ccctcggcgc cgaggccagc ttcgtggccc
      541 gcacccacga catggaccgc aaccacatga tggagaccat caagcgggcc cacgaccacc
      601 agggtgcgtc gatcgtggag atctaccaga actgcaacgt cttcaacgac ggggcgttcg
      661 gccagatcac cgccaagggc gcgcgcgacg agatgctcat cgacctccgc cacggggagc
      721 cgatcctgtt cggcgccgac cgccagcgcg gcgtggtgct cgacggcggc ggcgtgcgcc
      781 tcgccgaggt ggccgaggtc gggatggacg cggtgctggt ccacgacgag acccgcccgg
      841 acccctccat cgccttcatg ctgtcccgca tcgccaccgg cccccacgag ccgacccccg
      901 tcggcgtctt ccgagccgtg gcccgggacg agtacggcag ccgggtcaac cagcagctcg
      961 cggccgccca ggagcagcgc gggccgggca acctgcacga cctgctccga tccggcgcca
     1021 cgtgggaggt cgagtgatca ctcgatctcg agggcgtcgc cgaggctgat ccgcccggcg
     1081 gccaccacca gcgcgccgac gcccaggttc ccggcgcgct cggcgtgcac ctgcgtgagc
     1141 accgacgtgt cccggtcgag cccgggttgg ggtcgggtga cgatgacgca gcgatcgatc
     1201 tccttgacga cgtcggcgac gacgctgccc atccggatgc gggagcccac gaggtctcgc
     1261 tcgtcgccgc cgcggatgac cacgttgggc cggaaccgac ggacgtctcg agcgccgagc
     1321 gcctcctcgg ccacgatcga caggcgggtc cgcttcgagt cgtgccacac gccggcgggg
     1381 ccctcccact gctcgggtgc ctcgtcgtcc agccctgcgc cctcgacggc gtagtggccg
     1441 tgctcgtcgg gcgccggacg gcggagctcc acggggcgcc ccacccacgc ggacaggacg
     1501 tcgtcgtcgt cggtgaccga tccgtccggt agtcgcaccg ccatgcggtc gtcgcgggcg
     1561 acgggctcgg cgaacagcag ctggggcacc cgccgagcgg tgaggacgag gccggtggtc
     1621 cggtcgacga ggccccactg gcggtcaccg tcgatgccga gcgccccgac gtcgaccgcc
     1681 gtcagggcct cgccgatcat cgacttcacg gggtagcgcc acagctgggt cacctgcacc
     1741 gggccatggt ggcatcgccg gtaggttccg aggccatgga cctgcgcatc ttcaccgagc
     1801 cccagcaggg cgcctcctac gacacgctcc tggctgcggc ccgggccacc gaggccgcgg
     1861 ggttctcggc gttcttccgc tcggaccact acctcaagat gggcgacgcc tccggcctgc
     1921 ccggaccgac cgatgcctgg atcaccctcg ccggcctggc ccgggagacc gagtccatcc
     1981 ggctcggcac gctcgtcacc gcggcgacgt tccggctgcc cggcccgctg gccatcagcg
     2041 tcgcccaggt cgaccagatg tcgggcggac gcgtcgacct cggcatcggc acgggctggt
     2101 acgacgacga gcacaccgcc tatggcatcc cgttcccgcc gctgaaggag cgcttcgaga
     2161 tcctcgagga gcagctcgag atcctcaccg gcctgtggcg cacgccggtg ggggagacct
     2221 tcggcttcga cggtcggcac cacaccgtgt cgggctcccc ggcgctcccg aagccggccc
     2281 aggatggtgg accgccgatc atcatcggcg gtggcgggcc gaagaagacc ccgtcgctcg
     2341 ccgcccggtt cgccagcgag ttcaacctcc ccttcgcgcc gaaggacgtc tgggtgaccc
     2401 agcggggccg ggtgcaggac gcctgtcgcg acatcgaccg ggaccccgac gagctcacgt
     2461 actcggtcgc gctcgtggtg tgcgtcggcg aggacgaggc cgagatcgcc cgccgggccg
     2521 cgtccatcgg tcgcgaggtc gacgagctgc gggagaacgg cgtgtgcggc acgcccgcgg
     2581 aggccgtggc gcgcctgcag gagtggggcg aggccggcgc cgaacgggtc tacctgcagg
     2641 tcctcgacct ggacgacctc gaccacatcg agctgctggg gcgcgaggtc cggaccgcgg
     2701 tctgatcgtc acaggttctc gtcgaggaac cccacgaccc gtcgctccca ctcatcgggc
     2761 gcgacgccga tcccgtcggt gtggccggca ccgtccaccg tccagaccac cacccgctcg
     2821 ggggcgccgg cggcgatcca ggcggcggcg tggcgctcgt cctcgacctg cccggcggcg
     2881 atgagcagga ccggcacgtc ggcgcgagcg accgcggcgc gcagcacggt cgggcggggt
     2941 gcctcggtga gcaggtcggt cagcccgtac tgcagctgct cgatccgctc ctgcacggcg
     3001 ccgcgccacc cgtggacgtc ggacagccag tccttgtcgt cggcccggcg ggcggtggcg
     3061 ccctcggcga cgacgacccg gacgcggtgg tcggtggctg caccgatggc ctcctcgcca
     3121 cccatcgaga gcccgaggag gccgatccgg tcggggtcga cgtcgggtcg ggtggtgacg
     3181 tggtcgagcc cggcggcgat gtcggcgtcg ccccaccagc cgaagtccat cgcccgcccg
     3241 gtgctctccc cgtgcccgcg agcgtccatg agcagcacgc cgtagccgtg ctcgacgagg
     3301 accgtggcct gggcgagggc gccttcgtgg gtggagcccg ccccgtggcg gacgagcacc
     3361 gcagcaccgt tcgtccccgg gacccaccag gcggccagcg tcacgccgtc cgaggtgggc
     3421 agcgagacgg gctcggcatc cagcgggggc tcggctgccg tccgcccgga ggaggagtcc
     3481 gggacgttca ccacggcgac ggccgggccc acgacgacca gggtgaggag gaccaggacg
     3541 accatcccga gccaggtggg gatccgcccg cgccggcgca ggccagcggt ggcggacgcc
     3601 gtgccgaagc cgacgagccc gaggccgccc agcacggcgc cgaccgcgac gtacccgcgg
     3661 acgccgcccg gggagtcgag caggtgcggg aggaagccga caccgatcgc gagggcgagc
     3721 gccccgagcc ccgcagcgag cgctgctgca cgccgcaccc ggccgcggag cccgaggagc
     3781 aggccggtga cgatcgcggc gacgagcgcg acccgcagcg ccccgcccac cgtgctgcca
     3841 ccgagtgcca ggagggcggc cagcccgcct gcggcgaggg ccgctgagag ctcggtgccg
     3901 cgccatcggt cgaacgtcac gggtgcatcg tccgcgcgga cggcgggcgc gagaagtgcc
     3961 ctcggtcccg gtgggccggg atcccgatgc gtcgccgtca gcggaggggc ctgtgacgtt
     4021 ctgctcttgc cagcaggtcg actcagccgg atgctgcggc catggagcgt cgaactgcgt
     4081 tcctcctggt ggttgcgctg gtcgccgccg cagcgtgcga cgacggctcc cgcaccacgt
     4141 cggcgcccac cacgcagcga tctccggtgg tcgtcgacac cgacggagga tccgacgacg
     4201 cgatcgcact gctgtacctg ctccagcacc cggacgtcga cgtcatcggc atcaccgtct
     4261 cgggcaccgg catcgtccac tgcgacccgg gcgtcgccaa cgtcctcggc tgggtcgagc
     4321 tggccgccgc gggcgatgtc cccgtgtcct gcggccgtgc ggagcccgtg gccggagagc
     4381 gcagctttcc cgaggcgtgg cggacccagg ccgacggccg ctacgacggc atcctcccgg
     4441 tggggcgggg ggcagctgac ccacgaccgg cggcgaggct gatgcgggac ctggtcaccg
     4501 acgagggcgg gctcgtgacg gtccttgcgc tgggtccgct caccaacgtg gctgacgccc
     4561 tcgagtcgac gcccgagatg gccgaggcga tcggctcgct cgtggtcatg ggcggcgcct
     4621 tcgacgtccc gggcaacgtg gatctcccgg aggcgccggc ggccgcagcc accgagtgga
     4681 acctgtacgt ggacccagca gcggcgcagg ccgtcctcga cgcagacatc gaggtgacgt
     4741 tcgtgccgct ggacagcgcc gtcggcatcg acgcgttcga cgtgcgtgcg ctcgcgcggg
     4801 cggcgtcgag tcccgccgga cgggccgccg ccgacctcct cctctcgagc ccgtttttca
     4861 cctcaccggg gttcgcgctg tgggatccgc tcgccgcggc cgtcgtcgct gagcccggcg
     4921 cgttcgtgtc gagcgagcga ccgacccgga tcgtcgtgga cggacccgag gccggccgca
     4981 ccgtggccga tggtggcaga cccgtgctgg tcgcccggcc gcaacgggcg ggagcgttcg
     5041 tcgcggggct gctcggcgtc ttgtccggcg ccgggccgcc gctggacgtc gatcgctcgc
     5101 cggacctctc ggtcgccgtg ggggagggca cgtgcgtcgc gagcgaccag gacctcgccg
     5161 ccggccccgt cgtgctcgac gtcggcaccc gcggggcggt cgccatcggc gtcatggatc
     5221 ccgaccggga cctgggcgac gtcgaggccc tcctcgccag cggtgcacca gccgccgcgc
     5281 cagagtggtt cccgctcgtc gccctcctcc aagggggcgg gcccgtcgtg gcccgggtcg
     5341 atccgggcca gctggacgtg gtctgcatcg acgtgggtgc ggacggcgcg ctgcacgtcg
     5401 ccggccatgt cgagctgacg gtgagggcgt ccgacggccc gtagcgaggg cgtgcggacg
     5461 caacgcacca ggtccgggtc actagtttcg ccggcgatgc ccagcacgcc gctccccgcc
     5521 gtcgacgacc tcaccctcga ggagaagtgc cgcctgctcg gcggcgcctc cacctggcgg
     5581 acccacccga tcgagcgggt cggcatcccc gcggtgaaga tgtccgacgg gcccaacggc
     5641 gtgcggggcg acggcctcgg cgcccgccgc acgcccgggg tggtcatccc ggtgggcatc
     5701 gccgtcggtg cgacgtggaa ccccgccctg gccgccgagc tcggggacct cctgggcacg
     5761 gagtcccgac gcaagggcgc gcacgtcctg ctcgccccga ccgtgaacct gcagcggacc
     5821 cccatcggtg gtcgggtgtt cgagtgcctg tccgaggacc ccgagctcac cgcccggctc
     5881 gccgtgcccc tcgtcgaggc cgtccaggcc cacgacgtcg ccgtgacggt gaagcactac
     5941 gtggccaacg acaccgagat cgaccggatg agcgtcgacg tggacgtcga cgaccgggtg
     6001 ctccgcgagc tctacctgcg gcccttcgag gccacggtca ccgaggccga cgcctggggg
     6061 atcatgagcg cgtacaacca gcgtggcggg gagcactgcg cggcgagccg ggagctgctc
     6121 accgggatcc tgcgcgacga gtggggcttc gacgggttcg tggtctcgga ctggtacggc
     6181 tcccacgaca ccgtcgcgtc gatccgcgcc ggcctcacgg tgcccatgcc cggcccgagc
     6241 accatctacg gcgagcgcct gcaggccgcc gtggaagccg gcgacgtcga ggaggccctc
     6301 gtcgacgccc aggtcgagga cctgctccgg ctcatggccc gcgtgaaggt caccgagcgc
     6361 gacgccgagc agcgcgagga gacggtggac gacccggccg agcgagcact gtgccgccgg
     6421 gcggtgatcg agggcctggt cctgtgccgc aacgagggtg ggaccctgcc gatcgcgtcg
     6481 ggtgccagcg tggcggtcat cggccccaac gccgaggaca cccgcatcat gggcggagga
     6541 tccgccagcc tccaggcgct gcccaaccgg tcgctgctcg aggccctcac cgaccgggcc
     6601 ggctcggtcg tccacgagcc gggtgtgcgc atcgaccgcc tccccccacc cctgaccgaa
     6661 gcggtgctgc ggaccccgga cggggagccc ggcctgcggg tcgactaccg cgacggcctc
     6721 gacccggact cgcccatcgt cgtcaccgac gtgacgcccg agtccatgct ccgcttcttc
     6781 gggtccaccc cggagggcgt ggaccccgag cgcttccacg tcacggtcac cggcacgttc
     6841 gtgccggaac ggaccggcac gcacctgctg tcagcggtcc tcaccggcgc gggacggatc
     6901 gaggtcggcg acatcgccgt gctcgacgac cccgaccggg cgctgccgcg cgggccgctc
     6961 ttcttcggct tcggatccga ggagcaggag gccaccatcg agtgcgagga aggggtggcg
     7021 gtcccgatcc ggatcaccac gaccgggagg ggcggctacg ccgccatccg gctcggcgtg
     7081 cgggcaccgg agccgcccga catgctcgag cgcgccgtcg ccgctgcccg cgatgccgat
     7141 gtcgccgtcg tggtggtggg caccaacgac gagtgggaga ccgagggcga ggaccgcacc
     7201 tccatcgccc tccccggcga ccaggacgag ctgatccgcc gggtcgccga ggccaacccg
     7261 cggaccgtcg tggtcgtcaa cgccggatca ccggtgtcga tgccctggat cgacgacgtc
     7321 gctgccgtgc tcctcgccta cttcggcggc aacgagctgg ccgacggggt caccgacgtc
     7381 ctcctcggcg acgccgaccc cggtggccgc ctgccgctga cctacccgaa ggcgctggag
     7441 gacacgccgg cgtggcgctg gtacgccccg gtcgacggcg tgcagcacta cggcgagggc
     7501 ctgctcatgg gctaccgggg cttcgacgcc gccggcaccg agccgctgtt cccgttcggg
     7561 cacggcctgt cctacggcag ctctgagtgg ggcgaggcgt cgctgtcctc ctggatcgcg
     7621 gggccggggg acgggatcga cgtcaccgtg ccgatcaggg cgacggggga gcgcgccgcc
     7681 accgtggtgg tccaggccta cctgtcggga ccggacggct tcggtcgccc gcccaaggtg
     7741 ttggccgggt tcgccaagtc cacggtcgaa cccggcgcgg aggaggatgt cgtcgtgacc
     7801 gtcccgccgg aggcgttccg gcgctgggac ggcgacggct gggtcgtcga cgaggggacg
     7861 tgggaggtcg tcgtcgctgc gtcggcgacc gacgtccgcc agcgcctggc cctcgaggtc
     7921 ggctgaggcg ttccgcagcc gctgccagaa ccctccggcg accctcagtc cagggcgcgg
     7981 aagacgatgc cggtggccgg cttcggggcg aagaacgtgg tcttgggcgg catgcgctcg
     8041 ccgccgtggg agatcgcctg gatctgggcc acggtggccg gccgcatgag gacggcggcc
     8101 tgggcccggc cctcggccac ggcctcggtc acgttggcca cgccgtgctg gtagctgagg
     8161 tcgtgggccg gcagggcggc gcgggcctgc tcgagg
//
LOCUS       DATISU010000323        28163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_69798, whole genome shotgun sequence.
ACCESSION   DATISU010000323 DATISU010000000
VERSION     DATISU010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 28163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28163
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69798"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..952
                     /locus_tag="VLR27_12690"
     CDS             140..952
                     /locus_tag="VLR27_12690"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSP04358.1"
                     /translation="MPGPDLVELRAVAEDVATEAAEVLAASVDDHRAAIDTKSTSTDM
                     VTEMDRASEALIVRRLQELRPDDGIVGEEGAAHPGTSGVEWVVDPLDGTTNYLYRHPG
                     WNVSVGATVDGVPAVGAVVVPSLGDTFAAATGHGATRNGRPLRIGPPAPLATSLVGTG
                     FGYEPAAREAQAEALSRLIARIRDVRRMGAAAADLCSVACGRLDAYYEAGLAEWDRVA
                     ATVVAREAGARVEVLTATDLGPDCTIAAHPERFDELVDLLVEAGVLRRSALG"
     gene            1023..1703
                     /locus_tag="VLR27_12695"
     CDS             1023..1703
                     /locus_tag="VLR27_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP04359.1"
                     /translation="MATRILTVEDDERIRTAVKLALEDEGWTVAEADTGEAALEVFQR
                     APTDVVLIDIMLPGIDGFEVCRSVRRISDVPIVMVTARADTHDVVAGLEAGADDYLTK
                     PFAPKELSARIRALLRRTRAADHGHTHLRFGDLEIIPDEGVVKRDEEEVHLTKTEFRL
                     LNELASSPGRVFSREVLLERVWGYGYFGDGRLVDVHIRRLRTKIEADPANPRHVVTVR
                     GLGYKLQA"
     gene            1700..3310
                     /locus_tag="VLR27_12700"
     CDS             1700..3310
                     /locus_tag="VLR27_12700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSP04360.1"
                     /translation="MTEGHVSSADTSPSSTGPVTVPAPLPSPRPAPRSTEEVEEVHQD
                     TSGLRMPRLGLRARVTLAFAIGALLLSMGMSLLVLGVARSNLVSNSETGATRQMYINA
                     RIVRSNASPDGANIAELLGSLQTPEGSRPVLYQRCIPEEGNEGEEECGPEGRWSQQDP
                     EYGRSALPPELRETVISGTPAKMRFPHPTTGDKSLAIGVPIRDFDGAYFEIVSLESLD
                     ETLRDISFTLFGASIATVLAGAALGWYASRRILRPLGEVGVAARKLASGELDTRIRVL
                     VDPDLEPIITSFNGMAGTLEDRIQKDAQFSSDVSHELRSPLMTLQASLEVLENNRDDL
                     NERGQSALDLLTADLDRFRELVEDLLEMSRFDAGVMHLELEEVLLGEFVRQVVRANGS
                     DVPVEPSPDMVVDEDFGDVITALDKRRMARVLTNLLTNADKYGDGAVAVRIDADEDWV
                     WIYVDDRGEGVPEEDRERIFERFNRAAAAHRRGASTGVGLGLALVAEHVRLHGGRVWV
                     EDAPGAGARFAVELPRIEIDHGDEGDLA"
     gene            3307..3900
                     /locus_tag="VLR27_12705"
     CDS             3307..3900
                     /locus_tag="VLR27_12705"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HSP04361.1"
                     /translation="MTHPTRLLLLVVAVLLAGACTVPTNDDPVELSGSIVPDTTTSTS
                     TTSPETATRQAFAYFLETSDSGTVLRRVPRTVEAGTGVQGVLSNMFTQRPSEDVPLEQ
                     GLTSAIPESATLLSATPAEDDPSVLVVDVRGLFGNEGVQGTTLRNALAQIVWTATEGD
                     PILQVVFRQDGERRPALVDNLEGTEDPVDRDDYQRNT"
     gene            3945..4979
                     /locus_tag="VLR27_12710"
     CDS             3945..4979
                     /locus_tag="VLR27_12710"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HSP04362.1"
                     /translation="MRVGILTGGGDCPGLNAVIRAAVRKGIRVHGDEMVGFLDGYQGV
                     VDQRFMRLDVEALRGKLPIGGTILGTSRYQPFMDEDGVDRFRQGLADQGVDALVVIGG
                     EGTLGAARDLAELGFPVVGVPKTIDNDMGGTEMTFGFQTAVQICTDAIDRLHTTAESH
                     DRVMVVEVMGRHAGHIAVWSGIAGGATVTLIPEEPFDIDSVCEAILRRHESGRFASIV
                     VVAEGAVPAPGTLQLAAPEVDVYGHQRLGGIGTTIGGEIERRTGYETRVTILGHIQRG
                     GTPNAYDRVLATRYGVAAIDLVHEGRYGEMVALQSDRITSIPLVEAKRLGLKTVDPQL
                     YDVAQVFFAH"
     gene            5025..5849
                     /locus_tag="VLR27_12715"
     CDS             5025..5849
                     /locus_tag="VLR27_12715"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSP04363.1"
                     /translation="MADERHVLVTGANSGIGLATVLHLAEKGFRVTGSVRSEAKADIV
                     ATAADVAGVEVETVLLDVTDADACEQVMADLGTLHGLVNNAGYGLTGAIEDVPDDEAR
                     AVLETMVLAPMRLARLALPGMRTAGGGRIVNISSIYGRTATPLTGWYQGSKHALEGLS
                     DALRMEVAKDGVHVILVEPGGFKTNIWADLERDLANREGSRYESAYQRIGGLTSMWEP
                     LMGEPASCAKVIAKALQTPMPRARYLVGADAKVMAAASQLTPTMVKDRILRIAQGL"
     gene            5851..7350
                     /gene="glpK"
                     /locus_tag="VLR27_12720"
     CDS             5851..7350
                     /gene="glpK"
                     /locus_tag="VLR27_12720"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HSP04364.1"
                     /translation="MGVVVAIDAGTTGVRSMALSEAGLPVGMAYREFTQHFPRPGWVE
                     HDATEIWEAVRATLTELVHELEEPIAAIGITDQRETVVAWDRRTGEPRHRAIVWQDRR
                     TAARCDELADAGHLELVRRTTGLVLDPYFSGTKLEWLLRPSDQGGGGVETDEHLAVGT
                     IDTWLLWQLTGGQVHATEPSNASRTMLFDIGELAWSEELCDLLGVPGAALPEVRPSSG
                     RFGTTAVGVGVPAGIPISGVAGDQQAALFGQACFEPGMTKNTYGTGSFVLMNVGQECP
                     EPVEGLLTTVAWSLGSVDAASTSYALEGAIFVTGAAVQWLRDGLEIIDEAAETGPLAE
                     SVPDTGGVVLVPAFTGLGSPWWDPYARGAIVGITRGTTRAHLARATVESMVFQTRDVV
                     DAMSAASGREVLAMRVDGGASVMPLLLQLQADQLQVPVSRPTVQETTALGAAYLAGLA
                     EGVWSSPDEVRENWVLDVEVEPAADRDAVDAAHATWLRAVERARGWATE"
     gene            complement(7367..7546)
                     /locus_tag="VLR27_12725"
     CDS             complement(7367..7546)
                     /locus_tag="VLR27_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04365.1"
                     /translation="MALTHPTTTPEDHRAVAVAADPEAAQRAAEANAAIILVACLFAL
                     VGGTQLLVAVLGHFA"
     gene            complement(7621..8289)
                     /locus_tag="VLR27_12730"
     CDS             complement(7621..8289)
                     /locus_tag="VLR27_12730"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HSP04366.1"
                     /translation="METCIVRVWLPDRPGALGEVATRIGDAGGEIVGIDILERSAGRA
                     IDELAVQIPDVSAIPALVGRIRSVPGVDIEDLRVLDGTAHDPRADVLETAAVIVGARD
                     RSTLLESVVTHGRRVLGGEWAAVLRIGEGEVLAGDGDLPLARWLVAFVEGSRTSARVS
                     SGESGPDDVVWAPLPGTGLALVVGRAGAPFRARERRQVSALARIVDTRLLDLSRRLHP
                     SNQG"
     gene            8432..9046
                     /locus_tag="VLR27_12735"
     CDS             8432..9046
                     /locus_tag="VLR27_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP04367.1"
                     /translation="MGRRLTPRGEQRRGELLTYATERFATNGYHPTSVAELVDGLGVG
                     KGVFYWYFESKEDLFLEILRSGERDLRRVQLDAMGEETDPVARMELAIRASMEWWATH
                     RELYVLIEFARTEASFASGVRKGERVALEDTRRLVQEAMDSGAISDGNATVVAQAVLG
                     VSSVLARTQVLGRGRPPSEVADEVIDFCWHGLLGRDAGQTRRTA"
     gene            complement(9051..10238)
                     /locus_tag="VLR27_12740"
     CDS             complement(9051..10238)
                     /locus_tag="VLR27_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSP04368.1"
                     /translation="MEFRRITGLPPYVFTIIDGLKQEARRAGNDVIDLGFGNPDLPSP
                     QIAVEKLAEAAMNPKNHRYSQSKGIPKLRQAAADLYLRRFGVALDPETEVVATIGAKE
                     GYSHLCWVLLGPGDSALVPSPSYPIHLWGPLFAGASIREIPMGGVVDHGEELLDRIRH
                     QFLYSWPRPRVIVVSFPHNPTTKCVDLPFFQGLVDFAREQEVIVVHDNAYADLGFDGW
                     QPPSILQAEGAKECAVELYSMTKSFSMAGWRVAFMAGNPEVVGALSKLKSYLDYGTFQ
                     PIQIAATVTLNEATEYPKEINAVYQSRRDALCDGLDRIGWEMPRPGGTMFAWAPIPEP
                     YRHLGSVEFAKKLVTEANVATSPGVGFGPGGEGNIRFALIENDQRIQQAIRGMKKALP
                     ELG"
     gene            10271..10753
                     /locus_tag="VLR27_12745"
     CDS             10271..10753
                     /locus_tag="VLR27_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HSP04369.1"
                     /translation="MQTIDVVVEIPRGSRNKYEYDHERHVFRLDRRLFSATVYPADYG
                     FIPDTLGEDGDPLDALVIMEEPTFPGCWIQARPVGVFWMTDDAGPDAKIICVPAGDPR
                     WERVPEIEDLPDYLLHEIEHFFTVYKDLEPHKFSSTRGYEGSEAAWTEIRAAHERYVP
                     "
     gene            complement(10741..11724)
                     /locus_tag="VLR27_12750"
     CDS             complement(10741..11724)
                     /locus_tag="VLR27_12750"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSP04370.1"
                     /translation="MAAPADQDRAVGIDIGGTKMLGVVVDSADPTAVLAEARRPTPVG
                     AAALVDGIAELVDALDPGGAGPVGLGIPGLIDDEDVFRFGANLPGVIDLAVGEVVGAR
                     LGRHVVADNDATCATYAEFRGGAGVGWADGVLLTLGTGIGGGIVVDGRVRHGANGFAG
                     EPGHMLIDPSGPPCPCGRRGCWERYASGSGLGRLGRDAALAGRLAAVVELAGGDPEAV
                     RGEHVVAAVRSGDHDASLVLDEFVWWVAAGIANLVDLLDPGVVVLGGGVIEAEDVLLP
                     RIEGAVPDQVLARGHRPVVPIVAAHFGERAGAIGAAVLALESAHAERAQGT"
     gene            complement(11729..12877)
                     /locus_tag="VLR27_12755"
     CDS             complement(11729..12877)
                     /locus_tag="VLR27_12755"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04371.1"
                     /translation="MRPNHDPQEGHLVPVITEDDIRALAAVRSNGALVTSCYLDVDGG
                     RYVRPADYERALDEMVRRMRRRPEVDEAVQGDLERIVARIGEGFDRTTTRGVALFASD
                     AHDLFVVHELPVAVRNELVVNPTPAVGQLELVLQQSEKVAVLAADKQHARVLVYQLGE
                     LVEKVERTDDIGRDYDTTGEHDRGGVDQHRDEMTHQHLRHAAELAWSVYQSTGFDHLV
                     LAAAEGVASELEADLHPYLAERLRARLDVPPSAPEPELRRAALAAERAIEMAREAALV
                     EELRAAVNGDGRGVAGIGPVVDALAERRVDRLLVSDGYAVEGWRCDSCDRLATVGRTC
                     TCGAEMAEVPDLVEEAVEAALAQSCRVVVCDGNADLDVLGRVGALLRY"
     gene            complement(12919..14043)
                     /locus_tag="VLR27_12760"
     CDS             complement(12919..14043)
                     /locus_tag="VLR27_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04372.1"
                     /translation="MRVLRVQLDDDHLVDLHPAVSVVSGLSGSQRTTLRRAFDAVGAG
                     LEPPIPALIEAHGLLFDAAQADLDLLDLARHPGRAVAAPATLAASLPEDDAARLRTAE
                     RDVLLLSSDRIRAVDDLARAEVAAARPTHARLRDRAGSLRAGIARHEETRAEPVRTAL
                     DDVRDQQRMGGSVQATPVADALGAVGLAVADLGLPAGEVVRIAEDWLEERSVDAAWSV
                     GAEVELRAIEDVLALPEDPSGSHLQDLDARRARASRAAAANAEAVDRLDELRTELARR
                     HGIRPTAQELDERLLARLADHRPARLAGAVPLLLDGVFDHLTDVDATTVLDRLAGQAG
                     AVQVVVVDDHPAVAAWTQAVGLRRAACVEPAARPPAAAPT"
     gene            complement(14043..14387)
                     /locus_tag="VLR27_12765"
     CDS             complement(14043..14387)
                     /locus_tag="VLR27_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04373.1"
                     /translation="MDEDPADPADTPGPDGDAADPVQTGIDQFQRAALEAIRAGRAML
                     DAAESIIQDPAAAEAVVRSVGTVARNATEAVAGFATGRWPTPGTAPGDDPDVGDDPDE
                     DPPDGFERISVS"
     gene            complement(14417..15586)
                     /locus_tag="VLR27_12770"
     CDS             complement(14417..15586)
                     /locus_tag="VLR27_12770"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HSP04374.1"
                     /translation="MRILLFTGKGGVGKTTTAAATAVRCADAGQRTIVLSTDPAHSLG
                     DAFDTELGDLTVEITDGLWGQQLDAQDRMEEAWDDIQDYLQAVFRWGGVDEIEAEELS
                     VVPGLDEIFALTDIKRYAASGEWDTIVVDCAPTAETIRLLSLPGVLAWWMERLFPMGR
                     RVNKVVSPLLGTVTDLPMPGDDVFGAVERFYERLDGVKELLTDRTVTTARLVVNPERV
                     VVAEARRTFTYLSLFGYSVDAVIANRLLPDAVSDPWFEDWKQRDLEHLAVIEEGFAPV
                     PVLRAELAPHELVGVDRLREFGTALYGDDDPSSHRADVAPMRLERTEAGDVLSVDLPF
                     TERDELALGRREDELLVRVGPYRRSILLPDSLKRRQVVDAALRDGVLCVTFAPTR"
     gene            complement(15597..16037)
                     /locus_tag="VLR27_12775"
     CDS             complement(15597..16037)
                     /locus_tag="VLR27_12775"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSP04375.1"
                     /translation="MPDQATQHMHIEAPLERIWSVLTDFDAYPTWARDLKEATVVQRD
                     DEGRASLVRFRAAAMGRSTTYTLAYEWDDAPNRLPWKLVSGDIQKVLDGAYEFAERDG
                     GTDVTYHLRVDLAIPLPGFVKRRAEARIISTALRELREHVTTTP"
     gene            complement(16085..16321)
                     /locus_tag="VLR27_12780"
     CDS             complement(16085..16321)
                     /locus_tag="VLR27_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04376.1"
                     /translation="MTPPLWLVIIGLEFPAIIAMLDCVQRPADHFEGGAEDRVAWIRW
                     LVVAILLVPVLIGYGILLGYYYVVIRRNTPGSPR"
     gene            complement(16318..16812)
                     /locus_tag="VLR27_12785"
     CDS             complement(16318..16812)
                     /locus_tag="VLR27_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04377.1"
                     /translation="MSDSPPPGPPTEAAEPDDVEVHEGAALVQLSFVGTAALLVAAVA
                     GVLSPDGAGVLTAWVSGVLFAIGVVLFLWGYAVGVVRSREEQITLGGLFFLSGTAPKV
                     VRFRLRIAFAVQVAAAFVAASLRPYTAVAFAVLAPMLGLGAMAMWGARHGTFHRKDDA
                     GPGR"
     gene            complement(16809..17921)
                     /locus_tag="VLR27_12790"
     CDS             complement(16809..17921)
                     /locus_tag="VLR27_12790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSP04378.1"
                     /translation="MTHLLVTNDFPPKIGGIQTLLWELWRRLDPGSFTVLTTPHPDAE
                     AWDAAQPFRVERTPDPVLLPHPGLVRRIDALAEEIGADTVVLDPALPLGLLGPHLARP
                     YDVLLHGAEVTVPGRLPGSRLALRRVLLGARRVIAFGGYPAAEAEHAAGRSLPLAVVP
                     CGIDSSRFRPLDPTEKAEVRARHGLHDGPLVLSLSRLVPRKGMDVLIDASARLAARVP
                     ELQVAIAGSGRDRERLDRRVAATGAPVTFLGRVPEEDLALVHGAADVYAMLCRSRWGG
                     LEQEGFGIVFLEAAAAGVPQVAGDSGGASEAVLDGETGFVVDPTDDDAVAVALERLLS
                     DDDLRARMGEAARTRAVEHFDYDRLASQLAAAIEDP"
     gene            complement(17918..19069)
                     /gene="queG"
                     /locus_tag="VLR27_12795"
     CDS             complement(17918..19069)
                     /gene="queG"
                     /locus_tag="VLR27_12795"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HSP04379.1"
                     /translation="MLGATELRDRVAAAGRDHGLDEVGCTDVASFPEVRRSLDQRKAA
                     GQHAGMSFTFRNPRRSTEPDRLLRNARTLVVGAKSYLESRPERPVGPVAAVARYAWRD
                     HYAELRAGLEAMAEVLRDHGHRAAVFADDNALVDRAAAHRAGIGWWGRNSNLLIPGAG
                     SWFVLGSVVTDAALPVASAPVADGCRSCTRCVDACPTGAITADGVIDARRCLAWLVQD
                     VGVFPREHRVALGDRIYGCDDCQEVCPPGRRDVAAVDRSDVAVHLGSPPDAWVSVLEL
                     LDPDVADDELLERFGRWYIPRRDPRHLRRNALVVLGNVGDASDPRQRAAVTRWCRDGD
                     ELLRAHAVWAAARLGLCDLVDERRDDPSPLVRSEVAMAPSVPRAPGALA"
     gene            complement(19087..20640)
                     /gene="sepH"
                     /locus_tag="VLR27_12800"
     CDS             complement(19087..20640)
                     /gene="sepH"
                     /locus_tag="VLR27_12800"
                     /inference="COORDINATES: protein motif:HMM:NF040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HSP04380.1"
                     /translation="MHDLQLVGFTTDRRGLIFRTRAGSRPEASFVVPITDELVELVTQ
                     LADGDTLPTGESDVADGPGEDTDVVAPETEPDPRPRSQMSVRDIQARLRAGEPTSRIA
                     AEAGVEEDWIERFAPPVRAEQQRIVAQALDCHLQRARSAPSAVPLRRAVGMALADKGI
                     AYTVAAYEAAWSARLLGHDRWAVSFTYHHRGRERVATWTYEAGEGALTTADRTAAQLG
                     YVAPGRAADDAGDAVDGIVGDPAATATRTTTPETPTPEPATTTARTSTGKKATAEKAP
                     ARKAAAKQTATKKKATKKKAAKKAATKKQAARKAATKRKAAQKAATGKAPAKKVASKK
                     QAAAKKAPTAKPAPPEAQRPKEKVVVKTTGTRPPEPAPAERTPPPPTTGDVDVPSDHR
                     LPDEPPRPADVPVAVPPVVDRTPAPQSNGRPHPDASPSGPGRRAAPEADEVDPASARR
                     ERAAQRTASTVHFRSGSAAPVRVAPEPQPSRPAGDRPAADDRPRPERGETNGAARPAT
                     RRRRQLRAR"
     gene            complement(20718..20930)
                     /locus_tag="VLR27_12805"
     CDS             complement(20718..20930)
                     /locus_tag="VLR27_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04381.1"
                     /translation="MTPSQPLVIDCADCIGPTIGACDDCVVTFLCERDPDDAVVIDVG
                     EARALRLLADADLAPRLRHRSVERGA"
     gene            complement(21032..21262)
                     /locus_tag="VLR27_12810"
     CDS             complement(21032..21262)
                     /locus_tag="VLR27_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04382.1"
                     /translation="MTDSGDAPTPRYRCTRCGNLTRFDVTSTRRTRAFHHYSVGGELE
                     VEDVDVLDEVIEAVECRWCGNGEGVEELPADA"
     gene            21374..23068
                     /locus_tag="VLR27_12815"
     CDS             21374..23068
                     /locus_tag="VLR27_12815"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HSP04383.1"
                     /translation="MVAPTAPQARRARRRAPGQGSFDDLGTPLFDVTFCVLDLETTGG
                     SPTDDAICEIGAIKVRGGECLGTFHTFVDPGRDIPAQITVLTGLTSAMVRPAPAIEHV
                     LPSLLEFIGEAAFVGHNVRFDKGFLDAALVRTDRPRLPNPTVDTCALARRLVRDEVPN
                     CKLGTLAERLRLDHRPSHRALDDALATADLLHVLLERAAGFGVSTLDDLLELPSAAAH
                     PQAKKLALTDRLPRSPGVYLFKDATGRVLYVGKASDLRARVRSYFTSDTRRKVGGLLR
                     EAAAIDHIATTSALEAAVTEIRLIHELLPRYNRQGTTWAKAAYLKLTAEAFPRLSVVK
                     RVRADGATYLGPLSSARVARRVAEAVETALPVRRCTADPAREPRQAPCAPAQLGVSAC
                     PCAGDIDEEGYEPVAASVREALTTKPELVLDRLLERMEVLAGDERYEEAADMRDRAIA
                     FADAHRRVRRLDLVHRAGRLVVQLGDQRTVIEDGRLRHTEQADAPSLLSSVERDVDRE
                     HAAHRWVEPAAADELTVLAGWIERNAERIRIEHCDGTLCSPLPRLPDLPARSATGR"
     gene            complement(23055..23663)
                     /locus_tag="VLR27_12820"
     CDS             complement(23055..23663)
                     /locus_tag="VLR27_12820"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP04384.1"
                     /translation="MTDAAPMSDDPTRDRLIEAAAAVFAEKGYDGAGVQEIARRAGFT
                     TGAIYGRFRGKAELLLAAIEAHTTNELEQLFADHRFEGRVTDVITTVGSHLLTDPFDE
                     GQALLLEAFVAARRDPEVAELMRELLRQRATTFAALVEDAKASGLVDEGLDTDAIVRF
                     CHAVGFGFLLFGAIDLQPPRADAWELLIARLVGALGDPVTDQ"
     gene            23749..24699
                     /locus_tag="VLR27_12825"
     CDS             23749..24699
                     /locus_tag="VLR27_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HSP04385.1"
                     /translation="MAGGLVGLFDDVAALAKLAAASIDDVGAAAGRASMKAAGVVVDD
                     TAVTPAYVQGLAAERELPIIRRIATGSLRNKLLFILPVAILLSEFAPTLVEIILMFGG
                     AFLCYEGAHKILGAVKGDDHDHDVPAAVQGGDVEQATISGAIRTDFILSAEIMVISLK
                     EVIDEPFVSRIVIMVVVALLITVVVYGLVAAIVKMDDVGLRLAQRDSARSQQVGRTLV
                     KAMPIVLRWLSVIGTAAMLWVGGHILLVGAHELGWHTPYDLVHDLEELVHDTGSLSGV
                     LEWLVNTAASAVLGLAVGLVIATIVDKLPSRGRSPEHHPA"
     gene            complement(24707..25765)
                     /gene="trpD"
                     /locus_tag="VLR27_12830"
     CDS             complement(24707..25765)
                     /gene="trpD"
                     /locus_tag="VLR27_12830"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HSP04386.1"
                     /translation="MSAPAIEGLGGWTHILGHLVEGQDLRGEEAAAALASILAGEATP
                     AQLAAFIVALRLKGETVEEVAGMVGAMLDAAAPLELPPGTIDIVGTGGSPRRRVKALN
                     VSTMACLVAAAAGATVCKHGNLKASSTSGSFDLLGALGIGYDLDGPALAAVVRDVGVG
                     FCFARSFHPAMRHAGPVRAELGVPTVFNLLGPLSHPGRVRRQVIGVGDPRSLDLVAGA
                     LAARGSEHALVVHGDGAIDELALTGPTQVREVRDGEVLAPSTYAPADAGLSAVAVEAL
                     PGGDAGANADIARAVLAGEPGPARDIVVLNAGAGLYVAGLVDSIAEGARRAEAAIDAG
                     TAESLLARVVERTRAAGT"
     gene            complement(25788..26021)
                     /locus_tag="VLR27_12835"
     CDS             complement(25788..26021)
                     /locus_tag="VLR27_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HSP04387.1"
                     /translation="MDNSWSARAACHGLDPTIFFPADDDAASLAKAVCDSCPVREPCL
                     EYALGEREREGVWGGCTERERRRIIRRRRRAAS"
     gene            complement(26124..26378)
                     /locus_tag="VLR27_12840"
     CDS             complement(26124..26378)
                     /locus_tag="VLR27_12840"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP04388.1"
                     /translation="MVATKSDTERQRLVRELVDGRDRDTLGGRLLRTREVALLFEVSE
                     RAVTDWATKGRISSIRTPGGHRRYPADAVAGLLAAEGRTA"
     gene            complement(26492..27091)
                     /locus_tag="VLR27_12845"
     CDS             complement(26492..27091)
                     /locus_tag="VLR27_12845"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HSP04389.1"
                     /translation="MSRKLTLSDIADLREYERERADFRAHVIDLKRRRRVGLGPFVTV
                     LFENRDTIRFQIQEMARVEKLFTDEAIEGELRVYNPLVPEPGELALTLFIELTSELAL
                     RDWLPRLVGIERTLELRIGEGDDRLVIPCIPDEEHQEQLTREEITASVHYIHFELDPD
                     QVEAFAAGPVELALTHENYEHATTLGAETTAELLADLRP"
     gene            complement(27211..27633)
                     /locus_tag="VLR27_12850"
     CDS             complement(27211..27633)
                     /locus_tag="VLR27_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HSP04390.1"
                     /translation="MAELKGSKTEENLKEAFAGESQANRRYLYFAQKADVEGYPDVAA
                     LFRSVAEGETGHAFGHFDFLAEVGDPVTGEAVGTTADNLKSSIAGETYEYTEMYPGFS
                     KTAREEGFEEVAEWLETLARAEKSHAGRFQQGLESLDA"
     gene            complement(27680..28105)
                     /locus_tag="VLR27_12855"
     CDS             complement(27680..28105)
                     /locus_tag="VLR27_12855"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HSP04391.1"
                     /translation="MRSPSDLADAFRSSGRKLTPQRERIFRVLHDNPTHPTAEAVHAE
                     VVADMPSVSLRTVYATLHDLAEMGELVQLDLGTGSSRFDPNVSEHHHLVCDGCGRVQD
                     VDLAVPSFELDVDAARGFHVDATQIVLRGRCNACSVPAA"
BASE COUNT         3799 a        10168 c        10425 g         3771 t
ORIGIN      
        1 tcggcgggtt ggtcggcgcc gtgatcttcg ccgtcggcgt gggcatcgtg gccgtcctgg
       61 ccatgcgggc catgggcgag tggcgggtga tcgagcactc ccgggagcag gccgaccgcc
      121 gcggaccgat cagctgagcg tgcccggccc cgacctggtc gagctgcgcg ccgtcgccga
      181 ggacgtcgcc accgaggccg ccgaggttct cgccgcgagc gtcgacgacc accgcgccgc
      241 gatcgacacc aagtcgacgt cgaccgacat ggtcaccgag atggaccggg ccagcgaggc
      301 cttgatcgtc cggcgcctgc aggagctgcg gcccgacgac gggatcgtcg gcgaggaggg
      361 cgctgcccac ccggggacga gcggggtcga gtgggtcgtg gaccccctcg acggcaccac
      421 caactacctg taccgccacc cggggtggaa cgtgtcggtc ggggcgacgg tcgacggcgt
      481 ccccgcggtc ggcgccgtgg tcgtgccgtc cctgggcgac acgttcgcgg cggcgaccgg
      541 ccacggcgcc acccgcaacg gccgccccct gcgcatcgga ccgcccgccc cgctggccac
      601 cagcctggtc ggcaccggct tcggctacga gccggcggcg cgggaggccc aggccgaggc
      661 gctctcccgc ctgatcgccc gcatccgcga cgtgcgccgg atgggggcgg cggccgccga
      721 cctgtgctcg gtcgcctgcg ggcgcctcga cgcgtactac gaagccggcc tcgccgagtg
      781 ggaccgcgtc gccgcgaccg tcgtggcgcg ggaggccggg gcgcgggtcg aggtcctcac
      841 cgccaccgat ctcggtcccg actgcacgat cgccgcccac ccggagcgct tcgacgagct
      901 ggtggacctc ctcgtcgagg ccggcgtgct gcgtcgctcc gcactgggct gaacgaacct
      961 tttcctgcgg gttcgggcac atgcgcgcgc caccggcccc cgatgaggca cgatggggcg
     1021 atatggcgac gaggatcctc acggtcgagg atgacgaacg gatccgcacg gcggtcaagc
     1081 tcgccctcga ggacgagggc tggaccgtgg cggaggccga cacgggcgag gctgcgctcg
     1141 aggtgttcca gcgcgcgccc accgacgtcg tgctcatcga catcatgctc cccggcatcg
     1201 acggcttcga ggtctgccgc tcggtgcgcc gcatcagcga cgtccccatc gtgatggtga
     1261 cggcccgggc cgacacccac gacgtcgtgg ccggcctcga ggcgggcgcc gacgactacc
     1321 tcaccaagcc cttcgccccc aaggagctgt cggctcgcat ccgggcgctg ctgcgccgga
     1381 cccgggcggc cgaccacggt cacacgcacc tccgcttcgg cgatctcgag atcatccccg
     1441 acgagggcgt ggtcaagcgg gacgaggagg aggtccacct caccaagacc gagttccgcc
     1501 tgctgaacga gctggcctcc tcccccggcc gggtgttctc ccgcgaggtc ctgctcgagc
     1561 gggtgtgggg ctacgggtac ttcggcgacg gccggctggt cgacgtccac atccggcggc
     1621 tgcgcaccaa gatcgaggcc gatccggcca acccgcgcca cgtcgtcacc gtccggggac
     1681 tcggctacaa gctccaggct tgaccgaggg ccacgtgtcg tcggccgaca cctccccctc
     1741 cagcaccgga ccggtcaccg tcccggcacc gctcccgtcc ccgcgtcccg ccccgcgctc
     1801 gacggaggag gtcgaggagg tccaccagga cacctccggc ctgcgcatgc cgcgcctcgg
     1861 cctccgggcc cgggtgacgc tggccttcgc catcggggcg ctgctgctct cgatgggcat
     1921 gtccctgctc gtcctcggcg tcgcccgctc caacctggtg agcaacagcg agaccggcgc
     1981 gacgaggcag atgtacatca acgcccggat cgtgcgttcg aacgccagcc ccgacggcgc
     2041 gaacatcgcc gagctgctcg ggtcgctgca gacgccggag ggctcgcgcc ccgtgctcta
     2101 ccagcggtgc atccccgagg aggggaacga gggcgaggag gagtgcgggc cggagggccg
     2161 ctggagccag caggaccccg agtacgggcg cagcgcgctg ccgcccgagc tgcgtgagac
     2221 ggtgatctcg ggcacgccgg ccaagatgcg cttcccccac cccaccaccg gcgacaagag
     2281 cctggccatc ggcgtcccga tccgggactt cgacggcgcc tacttcgaga tcgtgtcgct
     2341 cgagtcgctc gacgagaccc tccgtgacat ctccttcacc ctgttcggcg cctcgatcgc
     2401 cacggtcctc gccggcgccg ccctgggctg gtacgccagc cggcgcatcc tgcgtccgct
     2461 cggcgaggtc ggcgtcgcgg cgcgcaagct ggcctccggc gagctcgaca cccggatccg
     2521 cgtcctcgtc gaccccgacc tcgagccgat catcacctcc ttcaacggca tggccggcac
     2581 cctcgaggac cgcatccaga aggacgccca gttctcctcc gacgtgtccc acgagctccg
     2641 ctcgcccctg atgaccctgc aggcctcgct cgaggtgctc gagaacaacc gcgacgacct
     2701 gaacgagcgg ggccagtccg cgctcgacct gctgaccgcc gacctcgacc gcttccggga
     2761 gctggtcgag gacctgctcg agatgtcccg cttcgacgcc ggcgtgatgc acctcgagct
     2821 cgaggaggtc ctgctcggcg agttcgtccg ccaggtcgtg cgggccaacg gctccgacgt
     2881 ccccgtcgag ccgtcccccg acatggtggt cgacgaggac ttcggcgacg tcatcaccgc
     2941 cctcgacaag cgccgcatgg cgcgcgtgct caccaacctg ctcaccaacg cggacaagta
     3001 cggcgacggc gccgtcgccg tgcggatcga cgccgacgag gactgggtct ggatctacgt
     3061 cgacgaccgc ggcgagggcg tgcccgagga ggaccgcgag cgcatcttcg agcggttcaa
     3121 ccgggccgcc gccgcccacc ggcgcggcgc cagcacgggg gtgggcctcg gcctcgccct
     3181 ggtggccgag cacgtgcgcc tgcacggggg acgcgtctgg gtcgaggacg cacccggcgc
     3241 cggcgcccgg ttcgccgtcg agctgccccg catcgagatc gaccacggcg acgagggcga
     3301 cctggcatga cgcaccccac caggctcctc ctcctcgtcg tcgccgtcct cctcgccggc
     3361 gcgtgcaccg tgcccacgaa cgacgatccc gtcgagctga gcgggtcgat cgtgccggac
     3421 accacgacct cgacgtcgac gacctcgccg gagaccgcca cccgccaggc cttcgcctac
     3481 ttcctcgaga ccagcgactc gggcacggtc ctgcggcgcg ttccccgcac cgtggaggcc
     3541 ggcaccggcg tccagggcgt gctgagcaac atgttcacgc agcggcccag cgaggacgtc
     3601 cccctcgagc agggtctgac cagcgccatc cccgagagcg ccaccctgct gtcggcgacg
     3661 ccggccgagg acgacccctc cgtgctcgtc gtcgacgtcc gggggctgtt cggcaacgag
     3721 ggcgtccagg gcacgaccct gcgcaacgcg ctggcccaga tcgtgtggac ggccaccgag
     3781 ggcgacccga tcctgcaggt cgtgttccgc caggacggcg agcggcgtcc cgcgctcgtg
     3841 gacaacctgg agggcaccga ggacccggtc gaccgggacg actaccagcg caacacctga
     3901 ccgggtgggc agcgtgcgcc gcccccgtcg gtaggttgcg ggccatgcgc gtcggaatcc
     3961 tcaccggtgg cggcgactgc cccggcctca acgccgtcat ccgtgccgcc gtccgcaagg
     4021 gcatccgcgt gcacggcgac gagatggtcg gcttcctcga cggctaccag ggcgtggtcg
     4081 accagcgctt catgcgcctc gacgtcgagg ccctccgcgg caagctcccc atcggcggca
     4141 cgatcctggg caccagccgc taccagccgt tcatggacga ggacggcgtc gaccggttcc
     4201 ggcagggctt ggccgaccag ggcgtggacg cgctcgtcgt catcggcggc gagggcacgc
     4261 tcggcgcggc acgcgacctg gccgagctcg gcttcccggt ggtcggggtc cccaagacga
     4321 tcgacaacga catgggcggc acggagatga ccttcggctt ccagaccgcc gtccagatct
     4381 gcaccgacgc catcgaccgc ctccacacca ccgccgagtc ccacgaccgg gtcatggtgg
     4441 tggaggtcat gggccgccac gccgggcaca tcgcggtgtg gtcgggcatc gccgggggcg
     4501 ccaccgtcac cctcatcccc gaggagccct tcgacatcga ctcggtgtgc gaagccatcc
     4561 tgcgccggca cgagtcgggc cgcttcgcct ccatcgtggt cgtggccgag ggcgcggtcc
     4621 ccgcacccgg cacgctccag ctggccgcgc cggaggtcga cgtctacggc caccagcgcc
     4681 tcgggggcat cggcaccacc atcggcgggg agatcgagcg gcgcaccggc tacgagaccc
     4741 gggtgaccat cctcggccac atccagcggg gtggcacccc caacgcgtac gaccgggtgc
     4801 tggccacgcg ctacggcgtg gctgccatcg atctcgtcca cgagggccgc tacggcgaga
     4861 tggtcgcgct gcagtccgac cgcatcacca gcatccccct cgtcgaggcc aagcgcctcg
     4921 gcctcaagac ggtggacccc cagctctacg acgtcgccca ggtgttcttc gcgcactgag
     4981 cgcgtcgcct ccggcgacgt cgtccgacct ggcaggctgc cgccatggct gacgagcgac
     5041 acgtgctggt gacgggagcg aactcgggga tcggcttggc gacggtgctg cacctcgccg
     5101 agaagggctt ccgggtgacg gggtcggtgc gatccgaggc caaagccgac atcgtggcga
     5161 ccgccgccga cgtggcgggg gtggaggtgg agacggtgct gctcgacgtc accgacgccg
     5221 acgcctgcga gcaggtcatg gccgacctcg ggaccctgca cgggctggtc aacaacgccg
     5281 gctacggcct gaccggtgcc atcgaggacg tccccgacga cgaggcccgc gccgtgctcg
     5341 agacgatggt gctggccccc atgcgcctcg cccggctcgc gctccccggc atgcggacgg
     5401 ccggcggcgg gcgcatcgtg aacatctcct ccatctacgg ccgcaccgcc accccgctca
     5461 ccgggtggta ccagggctcc aagcacgcgc tcgagggcct gtccgacgcc ctgcgcatgg
     5521 aggtggccaa ggacggcgtc cacgtgatcc tggtggagcc cggcggcttc aagaccaaca
     5581 tctgggccga cctcgagcgg gacctcgcca accgcgaggg cagccgctac gagtccgcct
     5641 accagcgcat cggtggcctg acctcgatgt gggagccgct gatgggcgag cccgcgtcgt
     5701 gcgccaaggt gatcgccaag gcgctgcaga cgccgatgcc ccgggcccgc tacctggtcg
     5761 gcgccgatgc caaggtgatg gcggcggcga gccagctgac accgacgatg gtgaaggacc
     5821 gcatcctgcg catcgcccag ggcctctgag atgggcgtgg tcgtggcgat cgacgccggc
     5881 accaccggcg tgcgctcgat ggcgctgtcg gaggctggtc tcccggtggg catggcctac
     5941 cgggagttca cccagcactt cccccggccc ggctgggtcg agcacgatgc gaccgagatc
     6001 tgggaggcgg tgcgggccac cctgaccgag ctggtccacg agctcgagga gccgatcgcc
     6061 gcgatcggca tcaccgacca gcgcgagacc gtcgtggcct gggaccggcg caccggcgag
     6121 ccgcgccacc gcgccatcgt ctggcaggac cgccgcaccg cggcgcgctg cgacgagctg
     6181 gccgacgccg ggcacctcga gctggtgcgc cggacgacgg gcctggtgct cgacccgtac
     6241 ttctccggca ccaagctcga gtggctgctg cgcccgagcg accagggcgg cgggggtgtc
     6301 gagaccgacg agcacctggc cgtcggcacc atcgacacct ggctcctctg gcagctcacc
     6361 ggcggacagg tccacgccac cgagccgtcg aacgcgtcgc gcaccatgct cttcgacatc
     6421 ggcgagctgg cctggtccga ggagctgtgc gacctgctcg gcgtgcccgg cgccgcgctg
     6481 cccgaggtcc gcccgtcgtc ggggcggttc gggacgacgg ccgtgggggt cggcgtgccg
     6541 gccggcatcc ccatctccgg ggtcgccggc gaccagcagg ccgcgctctt cggccaggcc
     6601 tgcttcgaac cggggatgac caagaacacc tacggcaccg gctcgttcgt gctcatgaac
     6661 gtgggccagg agtgccccga gccggtggag ggcctgctca ccacggtggc gtggagcctc
     6721 ggctcggtcg atgccgcctc gacgtcctac gcgctcgagg gcgccatctt cgtcaccggg
     6781 gccgccgtcc agtggctccg cgacgggctc gagatcatcg acgaggcggc cgagaccggc
     6841 ccgctggccg agtcggtgcc cgacaccggc ggcgtggtgc tcgtgccggc gttcaccggg
     6901 ctcggctcgc cgtggtggga tccctacgcc cggggcgcga tcgtcggcat cacccgaggc
     6961 acgacccggg cccacctggc ccgggccacg gtggagtcga tggtgttcca gacgcgcgac
     7021 gtggtcgacg cgatgtctgc ggccagcggc cgggaggtcc tcgccatgcg cgtggacggg
     7081 ggtgcctcgg tcatgccgct gctgctgcag ctgcaggccg accagctgca ggtgccggtg
     7141 tctcgaccga cggtgcagga gaccacggcg ttgggagccg cctacctcgc cgggttggct
     7201 gagggcgtgt ggtcgtcacc cgacgaggtg cgggagaact gggtgctcga cgtcgaggtg
     7261 gagcccgccg cggaccggga tgcggtcgat gccgcgcatg ccacgtggct gcgggcggtg
     7321 gagcgcgctc ggggctgggc cacggagtag cgctcggtcg tgggtcctag gcgaagtggc
     7381 cgaggacggc gaccaggagc tgcgtgccgc ccacgagggc gaacaggcag gcgacgagga
     7441 tgatcgcggc gttggcttcg gcggcgcgct gggcggcctc ggggtctgcg gcgaccgcca
     7501 cggcacggtg atcctccggc gtggtggtgg ggtgggtcag ggccatggcg cgctccctcc
     7561 ccgccacgca gcgtgctcag accaccccgg cggcaggaat cgtgcgcatg tgaccgatgg
     7621 ctacccctgg ttgctcggat ggagccgccg ggagaggtcg agcagccggg tgtcgacgat
     7681 gcgggcgagg gccgacacct ggcggcgctc gcgggcccgg aagggtgcgc cggcccggcc
     7741 caccaccagg gccagcccgg tgccggggag cggtgcccac accacgtcgt cgggtccgct
     7801 ctccccgctc gagacccgcg ccgaggtccg ggagccctcg acgaacgcga cgagccagcg
     7861 ggcgagtggg aggtcgccgt caccggccag gacctcgccc tcgccgatcc ggagcaccgc
     7921 ggcccactcc ccgccgagca cccgccggcc gtgggtgacc acgctctcga gcagcgtcga
     7981 ccggtcccgg gccccgacga tgacggcggc ggtctcgagc acgtccgccc gcgggtcgtg
     8041 ggcggtgccg tcgagcaccc ggaggtcctc gatgtcgacc ccggggaccg accggatccg
     8101 accgaccagc gccgggatgg ccgacacgtc cgggatctgc acggccagct cgtcgatcgc
     8161 ccggccggcg ctgcgctcga ggatgtcgat gccgacgatc tcaccgccgg catcgccgat
     8221 cctcgtcgcc acctccccga gcgcgccggg acggtcgggc agccacacgc gcacgatgca
     8281 tgtctccatg gcgcggacgg tacgggccgg tggtgaacgc ccggtttcgg gacgggggcg
     8341 tctccatgag cgacgaccat cggcgggatg gcgcgacccg cgaccggggc ggtcggtgaa
     8401 cttgaccgtc tggtcaagta ccgtgcggtc gatgggccgt cgactcacac cccgaggcga
     8461 gcagcgacgt ggcgagctcc tgacgtacgc gaccgagcgc ttcgccacca acggctacca
     8521 cccgacctcg gtggccgagc tggtcgacgg cctgggcgtg ggcaagggcg tcttctactg
     8581 gtacttcgaa tccaaggagg acctcttcct cgagatcctc cgctccggcg agcgcgacct
     8641 gcgccgggtg cagctggacg ccatgggcga ggagaccgac ccggtggcgc gcatggagct
     8701 ggccatccgg gcgtcgatgg agtggtgggc gacccaccgc gagctctacg tgctgatcga
     8761 gttcgcccgc accgaggcca gcttcgcctc cggcgtgcgc aagggcgagc gcgtcgccct
     8821 cgaggacacc cgccggctgg tgcaggaggc gatggactcg ggcgccatct ccgacggcaa
     8881 cgccaccgtc gtggcccagg cggtgctggg cgtcagctcc gtgctggctc gcacgcaggt
     8941 cctgggccgg ggtcgcccac ccagcgaggt ggccgacgag gtcatcgact tctgctggca
     9001 cggcctcctc ggccgggacg ccggccagac ccgccggacg gcataacccc tcagcccagc
     9061 tcgggcagcg ccttcttcat cccgcggatc gcctgctgga tccgctggtc gttctcgatg
     9121 agggcgaagc ggatgttgcc ctcgccgccc gggccgaagc cgacgccggg cgaggtcgcc
     9181 acgttggcct cggtgaccag cttcttcgcg aactccaccg accccaggtg ccggtagggc
     9241 tcggggatcg gcgcccaggc gaacatcgtc cctccaggcc ggggcatctc ccagccgatg
     9301 cggtcgaggc cgtcgcacag cgcgtcccgg cggctctggt agacggcgtt gatctccttg
     9361 gggtactcgg tggcctcgtt cagggtcacc gtcgccgcga tctggatcgg ctggaacgtg
     9421 ccgtagtcga ggtagctctt cagcttggag agcgcaccga cgacctcggg gttgccggcc
     9481 atgaaggcca cccgccaccc ggccatcgag aacgacttgg tcatcgagta gagctcgacc
     9541 gcgcactcct tggcgccctc ggcctgcagg atcgacggcg gctgccagcc gtcgaagccg
     9601 aggtcggcgt aggcgttgtc gtggacgacg atgacctcct gctcccgggc gaagtcgacg
     9661 aggccctgga agaacggcag gtccacgcac ttggtcgtgg ggttgtgggg gaacgacacg
     9721 acgatgacgc gcgggcgggg ccaggagtac aggaactggt gccggatgcg gtcgaggagc
     9781 tcctcgccgt ggtcgacgac gccgcccatc gggatctcgc ggatgctggc cccggcgaac
     9841 agcgggcccc agaggtggat cgggtagctg ggcgagggga cgagcgccga gtcgcccggg
     9901 ccgagcagca cccagcacag gtgcgagtag ccctccttgg ccccgatggt ggccacgacc
     9961 tcggtctcgg ggtcgagggc gacgccgaag cggcgcaggt acaggtcggc cgcggcctgg
    10021 cgcagcttcg ggatgccctt ggactgcgag tagcggtggt tcttcgggtt catcgccgcc
    10081 tcggccagct tctcgacggc gatctgcggg gacggcagat cggggttgcc gaagccgagg
    10141 tcgatgacgt cgttccctgc ccgccgtgct tcctgcttca gcccgtcgat gatcgtgaag
    10201 acgtagggcg gcagcccggt gatgcggcgg aactccatgg gcccacggta cctgcgacaa
    10261 gatgaccgac atgcagacga tcgacgtcgt ggtggagatc ccccggggaa gccgcaacaa
    10321 gtacgagtac gaccacgagc gccacgtgtt ccggctggac cggaggctgt tctcggccac
    10381 ggtgtacccg gcggactacg ggttcatccc cgacacgctc ggcgaggacg gcgaccccct
    10441 cgacgccctg gtcatcatgg aggagcccac cttcccgggc tgctggatcc aggcccgccc
    10501 ggtcggggtg ttctggatga ccgacgacgc cggacccgac gccaagatca tctgcgtgcc
    10561 ggcgggtgac ccccgctggg agcgggtccc cgagatcgag gacctcccgg actacctgct
    10621 ccacgagatc gagcacttct tcacggtcta caaggacctc gagccccaca agttctcctc
    10681 gacccgtggc tacgagggct ccgaggcggc ctggaccgag atccgcgccg cccacgagcg
    10741 ctacgtcccc tgagctcgct ccgcgtgggc tgactccaac gccagcaccg cagcaccgat
    10801 cgcgccggcc cgctcaccga agtgcgccgc cacgatcggc acgaccggcc ggtgcccccg
    10861 ggccagcacc tggtcgggca cggccccctc gatgcggggc agcaggacgt cctcggcctc
    10921 gatgacgccg ccaccgagca cgacgacccc ggggtcgagc aggtcgacca ggttggcgat
    10981 gccggccgcc acccaccaca cgaactcgtc cagcacgagc gacgcgtcgt ggtcacccga
    11041 tcgaaccgcg gcgaccacgt gctcgccccg gaccgcctcg gggtccccgc cggcgagctc
    11101 gaccaccgcc gccagccggc cggcgagggc ggcgtcccgg cccagccgac cgaggcccga
    11161 gcccgacgcg tagcgctccc agcagccccg ccgcccgcac gggcacggcg gaccgctggg
    11221 gtcgatcagc atgtgaccgg gctccccggc gaagccgttg gccccgtggc gcacgcgccc
    11281 gtcgacgacg atgccgccgc cgatgccggt gccgagggtg agcaggacgc cgtcggccca
    11341 cccgaccccg gccccacccc ggaactcggc gtaggtggcg caggtggcgt cgttgtcggc
    11401 gacgacgtgg cgcccgagcc gcgcccccac cacctcgccg accgccaggt cgatcacacc
    11461 ggggaggttg gcgccgaagc ggaagacgtc ttcgtcgtcg atgaggccgg ggatgcccag
    11521 cccgacgggg ccggcgccgc ccgggtcgag ggcgtcgacg agctcggcga tgccgtccac
    11581 gagggccgcc gcgccgaccg gggtcggccg gcgggcctcg gccagcaccg ccgtcgggtc
    11641 ggctgagtcg acgaccacgc cgagcatctt ggtgccgccg atgtcgatgc cgacggcgcg
    11701 gtcctggtcc gcaggcgcgg ccacgaggtc agtagcggag cagcgcgccg acgcgaccga
    11761 gcacgtcgag gtcggcgttg ccgtcgcaca ccaccacccg gcaggactgg gcgagcgcgg
    11821 cctcgacggc ctcctccacg aggtccggga cctcggccat ctccgcaccg caggtgcagg
    11881 tccgaccgac ggtggccagc cggtcgcagc tgtcgcagcg ccacccctcg acggcgtagc
    11941 cgtcggagac cagcagccgg tcgacgcgcc gctcggccag cgcgtcgacc acggggccga
    12001 tgccggccac gccccgaccg tcgccgttca ccgcggcgcg cagctcctcg acgagggcgg
    12061 cctcgcgcgc catctcgatg gcgcgctcgg cagccagcgc cgcccgccgc agctcgggct
    12121 cgggtgcgct cggcggcacg tcgaggcggg cgcgcagccg ttcggcgagg tacgggtgca
    12181 ggtcggcctc gagctcggag gccacgcctt ccgccgcggc gagcaccagg tggtcgaagc
    12241 cggtcgactg gtacacggac caggccagct cggcggcgtg gcgcaggtgc tggtgggtca
    12301 tctcgtctcg gtgctggtcc acgccaccac ggtcgtgctc accggtcgtg tcgtagtcgc
    12361 gcccgatgtc gtcggtgcgc tcgaccttct cgacgagctc gccgagctgg tacacgagca
    12421 cgcgggcgtg ctgcttgtcg gcggcgagca ccgccacctt ctcgctctgc tgcaggacca
    12481 gctccagctg cccgacggcc ggggtcgggt tgaccaccag ctcgttgcgg accgccaccg
    12541 gcagctcgtg gacgacgaac aggtcgtgcg cgtcgctggc gaagagcgcg acgccccggg
    12601 tcgtggtccg gtcgaacccc tctccgatcc gggccacgat gcgctcgagg tcgccctgca
    12661 ccgcctcgtc gacctccggg cgacgtcgca tccgacgcac catctcgtcg agggctcgct
    12721 cgtagtcggc ggggcgcacg tagcgaccgc cgtcgacgtc gaggtagcag gacgtgacga
    12781 gcgcgccgtt gctgcggacg gcggccaggg cacggatgtc gtcttcggtg atgacgggca
    12841 cgaggtgtcc ctcctggggg tcgtggttcg ggcgcatgct aggaccccgc acggcagccc
    12901 gggccgggcg ggaggtgttc atgtcggggc cgccgcgggt ggccgcgctg ccggttcgac
    12961 gcaggcggcg cgacgcaggc cgacggcctg ggtccaggcg gcgaccgccg ggtggtcgtc
    13021 gaccacgacg acctgcacgg ccccggcctg gccggcgagc cggtcgagga ccgtcgtcgc
    13081 gtccacatcg gtgaggtgat cgaagactcc gtccagcagc agcgggaccg cgccggccag
    13141 acgagcgggc cggtggtcgg cgaggcgggc cagcaggcgc tcgtcgagct cctgggcggt
    13201 gggccggatg ccgtggcgcc gcgccagctc ggtccggagc tcgtcgaggc gatcgacggc
    13261 ttcggcgttg gctgccgcgg ctcgggacgc gcgcgctcgc cgtgcgtcga ggtcctggag
    13321 atggctgccc gacgggtcct cggggagggc caggacatcc tcgatggccc gcagctccac
    13381 ctcggccccc accgaccacg cggcatccac gctgcgctcc tccaaccagt cctcggcgat
    13441 gcggaccacc tccccggccg gcagacccag gtccgcgacg gcgagaccga ccgccccgag
    13501 cgcatcggcg accggtgtcg cctgcaccga cccacccatc cgctgctgat ccctgacgtc
    13561 gtccagggcg gtccgcaccg gttcggcacg ggtctcctcg tgccgggcga tgccggcgcg
    13621 gagcgacccg gcgcggtcgc gcagccgggc atgcgtgggc cgcgccgcgg ccacctcggc
    13681 acgagcgagg tcgtcgacgg cccggatccg gtccgacgac aggagcagca cgtcgcgctc
    13741 ggcggtgcgc agccgggccg cgtcgtcctc gggcagcgag gccgccaggg tggcgggggc
    13801 ggcgacggcg cggcccgggt ggcgcgccag gtccaggagg tcgaggtccg cctgcgcggc
    13861 gtcgaagagg agcccgtggg cctcgatcag ggcggggatg ggcggttcga gcccggcgcc
    13921 gacggcgtcg aacgcccggc gcagggtcgt gcgctgcgac ccggagaggc ccgacacgac
    13981 cgacacggcc gggtggaggt ccaccaggtg gtcgtcgtcg agctggaccc gcagcacccg
    14041 catcagctga cggagatccg ctcgaacccg tcgggtgggt cctcgtcggg gtcgtcgccg
    14101 acgtcggggt cgtcgcccgg cgccgtcccg ggtgtcggcc accggccggt ggcgaagccg
    14161 gcgacggcct cggtggcgtt gcgggcgacc gtgcccaccg agcgcacgac cgcctccgcc
    14221 gcggccgggt cctggatgat ggactcggcc gcgtcgagca tggcccgccc ggcccggatc
    14281 gcctcgagcg cggcgcgctg gaactggtcg atgccggtct gcaccgggtc ggccgcgtct
    14341 ccgtcgggtc ccggggtgtc agcggggtcg gcggggtcct cgtccatgcg cgtcgccatg
    14401 ttgccagacc gcggggctac cgggtcgggg cgaaggtcac gcagaggacg ccgtcgcgga
    14461 gcgcggcatc gacgacctgg cgccgcttga gcgagtccgg cagcaggatc gaccggcggt
    14521 acgggccgac ccggacgagc agctcgtcct cccgccgacc gagggcgagc tcgtcgcgct
    14581 cggtgaaggg gaggtcgacg gagaggacgt cgccggcctc ggtgcgctcc aggcgcatgg
    14641 gcgccacgtc ggcccggtgg ctcgacgggt cgtcgtcgcc gtacagcgcc gtgccgaact
    14701 cgcgcaggcg gtcgaccccg acgagctcgt gcggcgccag ctcggcccgc agcaccggca
    14761 cgggggcgaa gccctcctcg atgacagcca ggtgttccag gtcgcgctgc ttccagtcct
    14821 cgaaccacgg atccgacacg gcgtcgggca gcagccggtt ggcgatgacc gcgtcgacgc
    14881 tgtagccgaa cagcgacagg taggtgaagg tccgccgggc ttcggccacg accacccgct
    14941 cggggttgac cacgaggcgg gcggtggtga cggtccggtc ggtgaggagc tccttcacgc
    15001 cgtcgaggcg ctcgtagaag cgctcgaccg ccccgaacac gtcgtcgccg ggcatgggca
    15061 ggtcggtgac cgtgccgagc agcgggctca cgaccttgtt gacccggcgc cccatcggga
    15121 acaggcgctc catccaccag gcgagcaccc cggggaggga caggagccgg atcgtctcgg
    15181 cggtgggagc gcagtcgacg acgatcgtgt cccactcccc cgatgccgcg tagcgcttga
    15241 tgtcggtcag ggcgaagatc tcgtccaggc cggggaccac cgagagctcc tcggcttcga
    15301 tctcgtccac cccgccccag cggaacacgg cctggaggta gtcctggatg tcgtcccagg
    15361 cctcctccat ccggtcctgg gcatcgagct gctgacccca gagcccatcg gtgatctcga
    15421 cggtcaggtc gccgagctcg gtgtcgaagg catcgccgag cgagtgggcc gggtcggtcg
    15481 acagcacgat ggtgcgctgg ccggcatcgg cgcatcggac cgcggtggcg gcggcggtgg
    15541 tggtcttgcc gacgccgccc ttgccggtga acagcaggat cctcacgccg ggccgatcat
    15601 ggcgtggtgg tcacgtgctc gcgcagctcc cggagggcgg tggagatgat gcgggcctcg
    15661 gcgcgccgct tcacgaagcc ggggagcggg atcgccaggt cgacgcggag gtggtaggtg
    15721 acgtcggtgc cgccgtcgcg ctcggcgaac tcgtaggccc cgtcgagcac cttctggatg
    15781 tcgcccgaca ccagcttcca cgggaggcgg ttgggggcgt cgtcccactc gtaggcgagc
    15841 gtgtaggtgg tggagcggcc catggctgcg gcccggaagc gcaccaggct ggcccgcccc
    15901 tcgtcgtcgc gctgcaccac cgtggcctcc ttcaggtcgc gggcccacgt cgggtacgcg
    15961 tcgaagtcag tgagcacgga ccagatccgc tcgagggggg cctcgatgtg catgtgctgg
    16021 gtggcctgat cgggcatgcg ggcgattctg cctcacggtc ggggccggca cccggctggt
    16081 cggatcagcg agggctgccc ggggtgttcc ggcggatgac gacgtagtag tagccgagca
    16141 ggatcccgta gccgatcagg acggggacca gcaggatcgc cacgaccagc cagcggatcc
    16201 aggcgacgcg gtcctcggcg ccgccctcga agtggtcggc ggggcgttgg acgcagtcga
    16261 gcatggcgat gatggccggg aactcgagcc cgatgatgac gagccagagc ggcggggtca
    16321 tcgaccgggc ccggcgtcgt ccttgcggtg gaaggtgccg tgccgggccc cccacatggc
    16381 catggccccg agccccagca tcggggccag cacggcgaag gcgaccgcgg tgtaggggcg
    16441 caggctggcg gcgacgaacg cggcggcgac ctggacggcg aacgcgatcc gcaggcggaa
    16501 ccggaccacc ttgggcgcgg tgccactgag gaagaagagg ccaccgaggg tgatctgctc
    16561 ctcgcgggag cgcaccacgc cgacggcgta gccccacagg aacagcacca cgccgatggc
    16621 gaacagcacg cccgagaccc aggcggtgag cacgccggcc ccgtcggggc tgagcacgcc
    16681 ggcgacggcg gcgacgagca gggcggccgt gccgacgaag gacagctgca ccagggccgc
    16741 gccctcgtgg acctcgacgt catcgggctc ggcggcttcc gtgggtgggc cggggggtgg
    16801 ggagtcgctc aaggatcctc gatcgccgca gccagctgcg acgccagccg gtcgtagtcg
    16861 aagtgctcga cggcccgggt ccgggccgcc tcccccatcc tggcccgcag gtcgtcgtcg
    16921 gacagcaacc gctcgagggc gacggcgacc gcgtcgtcat cggtgggatc gaccacgaac
    16981 ccggtctcgc cgtcgaggac cgcctcggac gcgccgccgc tgtcgccggc gacctggggg
    17041 accccggcgg ccgcggcctc gaggaacacg atgccgaagc cctcctgctc cagcccaccc
    17101 cagcggctcc ggcagagcat ggcgtagacg tcggccgcgc cgtggacgag ggcgaggtcc
    17161 tcctcgggca cccgcccgag gaaggtcacg ggtgcgccgg tggcggcgac ccgccgatcc
    17221 aggcgctcgc gatcgcggcc cgagccggcg atggccacct gcagctcggg gacgcgggcg
    17281 gccaaccggg cggacgcgtc gatgaggacg tccatgccct tgcggggcac gaggcggctg
    17341 aggctgagga ccagcgggcc gtcgtggagc ccgtggcggg cgcgcacctc ggccttctcg
    17401 gtcgggtcga gcggccggaa ccggctgctg tcgatgccgc agggcaccac ggcgagcggg
    17461 agggagcggc cggcggcgtg ctcggcctcg gccgccgggt acccgccgaa cgcgatgacc
    17521 cggcgagcac cgagcaggac ccggcgcagg gccaggcggc tgcccggcag gcgcccgggc
    17581 accgtgacct cggcgccgtg gagcaggacg tcgtagggac gggccaggtg gggcccgagc
    17641 aggccgagcg gcagcgcagg atcgagcacg accgtgtcgg caccgatctc ctcggccagg
    17701 gcgtcgatgc gccggaccag cccggggtgg ggcagcagca ccgggtcggg ggtccgctcg
    17761 acccggaacg gctgggcggc gtcccaggct tcggcgtcgg ggtgcggcgt ggtgagcacg
    17821 gtgaaggagc cggggtcgag ccggcgccag agctcccaca gcagcgtctg gatgccaccg
    17881 atcttgggtg ggaagtcgtt ggtgacgagc aggtgggtca cgccagcgct ccgggcgctc
    17941 gagggacgct cggggccatg gccacctcgg accggacgag ggggctgggg tcgtcgcgcc
    18001 gctcgtcgac gaggtcgcac aggccgagcc gggccgcggc ccagaccgcg tgggcgcgca
    18061 ggagctcgtc gccgtcccgg caccaccgcg tgacggcggc ccgctggcgc gggtcggatg
    18121 cgtccccgac gttgcccagg accacgaggg cgttgcggcg caggtggcga gggtcgcggc
    18181 gcgggatgta ccagcgaccg aaccgctcga gcagctcatc gtcggcgacg tcgggatcga
    18241 gcagctcgag caccgacacc caggcgtcgg gtggtgagcc caggtgcacc gcgacgtcgc
    18301 tgcggtcgac cgcggcgacg tcgcgccggc ccggcgggca gacctcctgg cagtcgtcgc
    18361 acccgtagat gcggtcgccg agggccaccc ggtgctcacg ggggaagacg ccgacgtcct
    18421 gcaccagcca ggccaggcac cgacgggcgt cgatcacgcc gtcggcagtg atcgcaccgg
    18481 tgggacaggc atcgacgcag cgggtgcagg accggcagcc gtcggcgacg ggcgccgacg
    18541 ccacgggcag cgccgcgtcg gtgaccaccg agccgaggac gaaccagctg ccggcaccgg
    18601 ggatgagcag gttgctgttg cggccccacc agccgatgcc ggcccggtgc gcggcggccc
    18661 ggtcgacgag ggcgttgtcg tcggcgaaca ccgcagcgcg gtggccgtgg tcgcgcagca
    18721 cctcggccat ggcctcgagg ccggcgcgca gctcggcgta gtggtcgcgc caggcgtacc
    18781 gggcgactgc ggcgaccgga ccgacgggcc gctcgggccg ggactccagg taggacttgg
    18841 ccccgaccac cagggtgcgg gcgttgcgca gcaggcggtc gggctcggtg gagcgccgcg
    18901 ggttgcggaa cgtgaaggac atgccggcgt gctggccggc ggccttgcgc tggtccaggg
    18961 accggcggac ctcggggaac gaggccacgt cggtgcagcc gacctcgtcg aggccgtggt
    19021 cccggccggc ggccgcgacg cgatcgcgca gctcggtggc accgagcacc ggtgcagcgg
    19081 accggttcac cgggcgcgga gctggcgtcg ccgacgcgtg gccgggcggg ccgccccgtt
    19141 ggtctcgccc cgttccgggc ggggacgatc gtccgcggcg ggacggtcac ctgccgggcg
    19201 ggagggctgg ggctctgggg ccacccggac cggcgcagcc gagccgctgc ggaagtgcac
    19261 ggtggaggcc gtgcgctggg cggcacgttc gcgacgagcc gacgccgggt cgacctcgtc
    19321 ggcttcgggc gcggccctgc gtcccgggcc ggacggggag gcgtcggggt gcgggcggcc
    19381 gttcgactgc ggcgccgggg tgcggtcgac gacaggaggc accgccaccg ggacatcggc
    19441 gggccgaggc ggctcgtcgg gcaggcggtg gtcgctcggc acgtcgacgt cgccggtggt
    19501 ggggggtggg ggcgttcgct cggcaggagc cggctcgggt ggcctcgtcc ccgtcgtctt
    19561 gacgacgacc ttctccttcg ggcgctgcgc ctccggtggt gccggcttcg cggtgggggc
    19621 cttcttcgcc gcagcctgct tcttgctcgc gaccttcttc gccggcgcct tcccggtcgc
    19681 cgccttctgg gcggcctttc tcttggtcgc cgccttccgg gcggcctgct tcttggtcgc
    19741 cgccttcttg gcggccttct tcttggtggc cttcttcttg gtcgcggtct gcttcgccgc
    19801 agccttcctg gccggcgcct tctcggctgt ggccttcttg cccgtcgacg tccgagcggt
    19861 ggtcgtcgcc ggctccgggg tcggggtctc cggggtggtg gtgcgggttg cggtggccgc
    19921 cgggtcaccc acgatgccgt cgacggcatc cccggcgtcg tcggccgccc ggccgggcgc
    19981 cacgtagccg agctgggcgg cggtgcggtc ggcggtggtc agggctccct cgccggcctc
    20041 gtaggtccaa gtggccaccc gctcccgacc gcggtggtgg tacgtgaagc tcaccgccca
    20101 gcggtcgtgg ccgagcagtc gggccgacca cgctgcttcg taggccgcca cggtgtaggc
    20161 gatgcccttg tcggccaggg ccatgccgac ggcgcggcgg agcgggaccg ccgagggcgc
    20221 cgaccgggcc cgctggaggt ggcagtcgag ggcctgggcc acgatgcgct gctgctcggc
    20281 ccgcaccggc ggggcgaacc gctcgatcca gtcctcctcc acccccgctt cggcggcgat
    20341 gcgcgaggtc ggctcgccgg cccggagccg ggcctggatg tcgcgcaccg acatctggga
    20401 ccgcggcctc gggtcgggct ccgtctccgg tgccacgacg tcggtgtcct cgcccggccc
    20461 gtcggcgacg tcggattcgc cggtgggcag ggtgtcaccg tcggcgagct gggtgaccag
    20521 ctcgaccagc tcgtcggtga tcgggacgac gaagctcgcc tccggtcgcg acccggcacg
    20581 ggtgcggaag atgaggccac ggcgatccgt ggtgaagccg acgagctgga gatcgtgcat
    20641 ccggaggcac ggcaatcgga caagtgccgc gggcgggcac ggtaccaccg cattccgtac
    20701 agaacccgcc ccaagggtca ggccccgcgc tcgaccgagc ggtgccggag acggggtgcg
    20761 aggtccgcgt cggcgaggag ccggagcgct cgcgcctccc ccacgtcgat caccaccgcg
    20821 tcgtcgggat cgcgctcgca caggaacgtc acgacgcagt cgtcgcaggc gccgatggtg
    20881 gggccgatgc agtcggcgca gtcgatcacc agcggctgcg agggggtcat cggtggcatg
    20941 gatcctccag agcgaatcgc gcgccccggg agatccgggg ggcacgtcga acgctacgaa
    21001 gggggtctga caggaacgga tcggcgcgcg ctcaggcgtc ggcgggcagc tcctcgacgc
    21061 cctcgccgtt gccgcaccag cggcactcca ccgcctcgat cacctcgtcg agcacgtcga
    21121 cgtcctcgac ctcgagctcg ccaccgacgg agtagtggtg gaacgcgcgg gtccggcggg
    21181 tgctggtgac gtcgaagcgg gtgaggttgc cgcagcgggt gcagcggtaa cgaggcgtgg
    21241 gggcatcgcc ggagtcggtc acggggcgag cgtaccgatc ggcatccacc ggaccgagtc
    21301 cgcccgcctg ccgccacggg cgcctccgat ccccgcttga cagacgaaca cgcgttcggt
    21361 tcactccgac gccatggtcg ctcccaccgc tccgcaggcc cgtcgagcac gccgccgggc
    21421 cccggggcag ggcagcttcg acgacctcgg gaccccgctg ttcgacgtca ccttctgcgt
    21481 gctggacctg gagaccaccg gcggctcccc caccgacgac gccatctgtg agatcggggc
    21541 gatcaaggtg cgcggcggcg agtgcctcgg gaccttccac acgttcgtcg acccgggccg
    21601 cgacatcccg gcccagatca ccgtgctcac cgggttgacc tcggccatgg tgcgcccggc
    21661 gcccgccatc gagcacgtgc tgccgagcct gctcgagttc atcggcgagg ccgcattcgt
    21721 cggccacaac gtgcgcttcg acaagggctt cctggacgcc gccctggtcc gcaccgaccg
    21781 accccgcctg cccaacccca ccgtcgacac gtgcgcgctg gcccgccggc tcgtgcgcga
    21841 cgaggtgccc aactgcaagc tcggcaccct ggccgagcgg ctccgcctcg accaccgccc
    21901 cagccaccgg gccctcgacg acgcgctcgc caccgccgac ctcctgcacg tgctgctgga
    21961 gcgggccgcc gggttcggcg tgtccaccct cgacgacctg ctcgagctgc cgagcgccgc
    22021 cgcccacccc caggccaaga agctggccct caccgatcga ctcccccgct cgcccggcgt
    22081 ctacctgttc aaggacgcca ccggccgggt gctgtacgtg ggcaaggcct cggacctgcg
    22141 ggcccgggtg cgcagctact tcacgtccga cacccgccgg aaggtcggcg gtctcctgcg
    22201 ggaggcggcc gccatcgacc acatcgccac gacctcggcc ctcgaggcgg cggtcaccga
    22261 gatccggctc atccacgagc tgctcccccg ctacaaccgc cagggcacga cctgggccaa
    22321 ggccgcctac ctcaagctga ccgccgaggc gttcccccgg ctgtcggtgg tgaagcgggt
    22381 gcgcgccgac ggcgccacct acctcgggcc gctgtcgtcg gcccgggtcg cccggcgcgt
    22441 ggccgaggcg gtggagaccg ccctgcccgt gcggcgctgc accgcagacc ccgcccgcga
    22501 gccccgacag gcaccgtgcg cgcccgccca gctcggcgtg agcgcgtgcc cctgcgcggg
    22561 tgacatcgac gaggagggct acgagcccgt cgccgcctcg gtccgcgagg cgctcaccac
    22621 gaagccggag ctggtgctcg accggctgct cgagcggatg gaggtgctgg ccggcgatga
    22681 gcgctacgag gaggccgccg acatgcggga ccgggcgatc gccttcgccg acgcccaccg
    22741 gcgggtccgc cgcctcgatc tggtccaccg cgccggtcgg ctcgtcgtgc agctcggcga
    22801 ccagcgcacg gtcatcgagg acggccgcct ccgccacacc gagcaggccg atgcgccgtc
    22861 gctgctgagc agcgtcgagc gcgacgtgga tcgcgagcac gcagcccacc gctgggtcga
    22921 gcccgccgcg gccgacgagc tcacggtgct ggccggatgg atcgagcgca acgccgagcg
    22981 gatccgcatc gagcactgcg acggcacgct gtgctcgccg ctgccccgcc tgcccgacct
    23041 cccggcccgg tcggctactg gtcggtgacc ggatcgccca gggcgccgac gagccgggcg
    23101 atcagcagct cccaggcatc ggcacgaggc ggctgcaggt cgatggcacc gaacagcagg
    23161 aacccgaacc cgacggcgtg gcagaaacgg acgatggcgt cggtgtcgag gccctcgtcc
    23221 accaggccgc tggccttggc gtcctcgacc agggcggcga aggtcgtggc ccgctggcgg
    23281 agcagctcgc gcatgagctc ggcgacctcg gggtcgcgcc gggcggccac gaacgcctcg
    23341 agcagcaggg cctggccctc gtcgaaggga tcggtgagca ggtgcgagcc gacggtggtg
    23401 atcacgtcgg tgacccggcc ctcgaagcgg tggtcggcga agagctgttc gagctcgttg
    23461 gtggtgtggg cctcgatggc ggcgagcagc agctcggcct tgccgcggaa gcgcccgtag
    23521 atggcgccgg tggtgaagcc ggcccgccgg gcgatctcct gcacgccggc gccgtcgtag
    23581 cccttctcgg cgaacaccgc cgccgcggcc tcgatgagac gatctcgcgt cggatcgtcg
    23641 ctcatgggcg cggcatcggt catggaccga ggataacggt ggttatcacc ggtggccacg
    23701 atctcgcgct taggttctgc gccttcgtcg gcaacgagag gatgcgagat ggctggcggt
    23761 ctggtcgggc tgttcgatga cgtcgctgcg ctggccaagc tggccgccgc gtccatcgac
    23821 gacgtcggcg ctgcggccgg acgggccagc atgaaggcgg ccggcgtcgt ggtggacgac
    23881 accgcggtca cgcccgccta cgtccagggc ctggcggccg agcgcgagct gccgatcatc
    23941 aggcggatcg ccaccgggtc gctccgcaac aagctgctgt tcatcctgcc ggtggcgatc
    24001 ctgctgagcg agttcgcgcc gaccctcgtc gagatcatct tgatgttcgg cggtgccttc
    24061 ctctgctacg agggcgccca caagatcctg ggggcggtga agggcgacga ccacgaccac
    24121 gacgtccccg ctgcggtgca gggcggcgac gtcgagcagg ccaccatctc cggggcgatc
    24181 cggaccgact tcatcctctc ggccgagatc atggtgatct cgctgaagga ggtcatcgac
    24241 gagcccttcg tctcccgcat cgtgatcatg gtcgtcgtcg cgctgctcat cacggtcgtc
    24301 gtgtacggcc tcgtcgccgc catcgtgaag atggacgatg tcggcctccg cctggcccag
    24361 cgggattcag ctcgctcgca gcaggtcggc cgcaccctgg tcaaggcgat gccgatcgtc
    24421 ctgcgctggc tctcggtcat cggcacggcc gccatgctct gggtgggtgg gcacatcctg
    24481 ctggtgggcg cccacgagct cgggtggcac accccctacg acctggtgca cgacctcgag
    24541 gagctggtcc acgacaccgg atcgctcagc ggggtgctgg agtggctggt gaacaccgcc
    24601 gcctcggccg tcctcggact ggcggtcggg ctggtgatcg ccaccatcgt cgacaagctg
    24661 ccgtcccgcg gccgctcgcc ggagcaccac ccggcctgag acgaggtcag gtcccggccg
    24721 cgcgggtgcg ctcgaccacg cgggccagca gggactcggc cgtgccggcg tcgatcgccg
    24781 cctcggcccg gcgggctccc tcggcgatgc tgtcgacgag gccggcgacg tacaggccgg
    24841 ccccggcgtt gagcacgacg atgtcccggg ccggaccggg ctcgccggcc agcaccgcgc
    24901 gggcgatgtc ggcgtttgcc ccggcgtcgc cacccgggag cgcctcgacg gccactgcgg
    24961 acagcccggc gtcggccggg gcgtacgtgg acggcgccag gacctcaccg tcccgcacct
    25021 cccgcacctg ggtcggcccc gtgagggcga gctcgtcgat cgccccgtcg ccgtggacca
    25081 ccagggcgtg ctcgctgccc cgcgccgcga gggcgccggc caccaggtcg agcgagcgcg
    25141 ggtcgccgac gccgatcacc tgccgacgga ctcgccccgg gtgggacagc ggcccgagca
    25201 ggttgaacac ggtgggcacg ccgagctcgg cgcgcaccgg accggcgtgg cgcatggcgg
    25261 ggtggaagga gcgggcgaag cagaagccga ccccgacgtc gcggacgacg gcggcgagcg
    25321 ccgggccgtc gaggtcgtag ccgatgccga gggcgcccag gaggtcgaag gagcccgacg
    25381 tcgacgacgc cttcaggttc ccgtgcttgc acacggtggc cccggcggcg gccgccacga
    25441 ggcaggccat cgtggacacg ttcagcgcct tcacccggcg acggggggat ccgccggtgc
    25501 cgacgatgtc gatcgtcccc gggggcagct cgagcggggc ggcggcgtcg agcatggcgc
    25561 cgaccatgcc ggcgacctcc tccacggtct cgcccttgag ccgcagggcc acgatgaacg
    25621 cggcgagctg ggccggcgtg gcctcgcccg cgaggatcga ggccagggcc gcggccgcct
    25681 cctcgccccg caggtcctga ccctcgacga gatggccgag gatgtgcgtc cagcccccga
    25741 gcccttcgat ggcgggcgcg ctcacggccg gatctccccc cggcccacta cgaggccgcc
    25801 cgccgccgcc ggcggatgat ccgtcggcgc tcgcgctcgg tgcagccacc ccagacaccc
    25861 tctcgctccc gctcgccgag cgcgtactcg aggcacggct cgcgcaccgg gcaggagtcg
    25921 cacaccgcct tggccaggct cgctgcgtcg tcgtcggccg ggaagaagat cgtcggatcc
    25981 agcccgtggc aggcagcccg tgcgctccag ctgttgtcca tgctcgctcc gccgggatgg
    26041 gggtcggcgc cggcggcgcc gaggttggca cccctcgcgc cagcggcaca aggacccggg
    26101 cgggccgggt ggcgcggtgc gcgctaggcg gtgcgacctt cggcggcgag caggccggcg
    26161 acggcgtcgg ccgggtagcg gcgatggcca cccggggtgc ggatgctcga gatgcggccc
    26221 ttggtggccc agtcggtcac cgcccgctcg gacacctcga acaggagggc gacctcgcgc
    26281 gtgcgcagca ggcggcctcc gagggtgtcg cggtctcggc cgtcgaccag ctcgcgcacc
    26341 agtcgctgcc gctccgtgtc actcttcgtc gccaccatgg tccggatggt agagaagaac
    26401 cggaaccact gcaagtggtt cctccctcac gtaacgtagt cgtcggtacg gcccggtcgg
    26461 ccgaaatcgc ccggaaccgg cgggcccgga gctagggacg cagatcggcg agcagctcgg
    26521 ccgtcgtctc cgcaccgagc gtggtggcgt gctcgtagtt ctcgtgcgtc agggccagct
    26581 cgaccgggcc ggcggcgaag gcctcgacct ggtccgggtc gagctcgaag tggatgtagt
    26641 ggaccgaggc ggtgatctcc tcgcgggtga gctgctcctg gtgctcctcg tcggggatgc
    26701 acgggatgac gaggcggtcg tcgccctcgc cgatgcgcag ctcgagggtc cgctcgatgc
    26761 cgacgaggcg gggcagccag tcgcgcagcg ccagctcgga ggtcagctcg atgaacaggg
    26821 tgagggccag ctcaccgggc tccggcacca gcgggttgta cacccgcagc tcgccctcga
    26881 tcgcctcgtc ggtgaacagc ttctcgaccc gtgccatctc ctggatctgg aagcggatcg
    26941 tgtcccggtt ctcgaacagc accgtgacga agggaccgag gccgacccgt cgccgccgct
    27001 tcagatcgat cacgtgggct cggaagtcgg cacgctcgcg ctcgtactcc cgaaggtcgg
    27061 cgatgtcgga gagggtcagc ttgcggctca tcggtggtcc tcggatcggc ggggggattc
    27121 ggtcgggaga gggtgacgag caaggggccg ggtcacgacc cggcccctcc gcacgtcatc
    27181 aggtgccgga cgggtggtcc ggcggctcgc tcaggcgtcg agcgactcga ggccctgctg
    27241 gaagcggccg gcgtggctct tctcggcgcg agccagcgtc tccagccact cggcgacctc
    27301 ttcgaagccc tcctcgcggg cggtcttcga gaagccgggg tacatctcgg tgtactcgta
    27361 ggtctcaccg gcgatggagg acttgaggtt gtcggcggtg gtgccgacgg cctcgcccgt
    27421 gaccgggtcg ccgacctcgg cgaggaagtc gaagtgaccg aacgcgtggc cggtctcgcc
    27481 ctcggcgacg gagcggaaca gcgccgccac gtccgggtag ccctcgacgt ccgccttctg
    27541 ggcgaagtac aggtagcggc ggttcgcctg gctctcgccg gcgaaggcct ccttcaggtt
    27601 ctcttcggtc ttggagccct tgagctcagc catgggtgtt gctcctcggt tgcttgcttg
    27661 tggttactgg gggatgtcgt caggccgccg ggacgctgca tgcgttgcag cgaccccgca
    27721 ggacgatctg ggtggcgtcg acgtggaagc cgcgggcggc gtcgacgtcg agctcgaagc
    27781 tggggacggc gaggtcgacg tcctgcacgc gcccgcagcc gtcgcacacc aggtggtggt
    27841 gctcggagac attgggatcg aagcgcgacg acccggtgcc gaggtcgagc tggacgagct
    27901 cgcccatctc ggccaggtcg tgcagcgtcg cgtagacggt gcgcaggctc accgagggca
    27961 tgtcggcgac gacctcggcg tgcacggcct cggccgtggg gtgcgtgggg ttgtcgtgca
    28021 ggacgcggaa gatgcgctcg cgctggggcg tcagcttgcg gccggaggac cggaaggcgt
    28081 cggcgaggtc ggacggggag cgcatcggtc tcccaccata ggtcaaggat tgctcatcga
    28141 caatcgttgt cgatcatcaa cga
//
LOCUS       DATISU010000324        13657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_163002, whole genome shotgun sequence.
ACCESSION   DATISU010000324 DATISU010000000
VERSION     DATISU010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 13657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13657
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163002"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VLR27_12860"
     CDS             complement(<1..245)
                     /locus_tag="VLR27_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04392.1"
                     /translation="MSHRSTNEPIGALVSVVEQARDVTTAQRRDLAGWVAGLLALVTT
                     AATFLDWGRSGQRVRTSYELVDVADRAGVLADDAAWAA"
     gene            complement(268..930)
                     /locus_tag="VLR27_12865"
     CDS             complement(268..930)
                     /locus_tag="VLR27_12865"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSP04393.1"
                     /translation="MTAERDPFPRTERTTIGRSPDRGTHDRAVAEAILDEGMLCHVGL
                     VTDHGPVVIPTTYARRGDELLIHGSPAATWLRATGKGEPVCVTVTLLDGLVLARSTFH
                     HSMNYRSVVVFGRAEPITDHDEKAAALDAIVEHLVPGRTAEVRPMRDDEIRSTLVVRL
                     ALDEASTKIRTGGPIDDDADADLVEVWAGVLPVRAAFGDPEPDDLAAAVLPPPSVVDY
                     GR"
     gene            complement(976..1287)
                     /locus_tag="VLR27_12870"
     CDS             complement(976..1287)
                     /locus_tag="VLR27_12870"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HSP04394.1"
                     /translation="MSTPLIVIIGLGLGTYALKSAGPLLLGGRRLPAPVGRIAQQLPA
                     ALLAALVVVSTVAGDRALVLDARLAGVVAGGVALRLRAPFIVVVAVAVAVTGVVRAVA
                     G"
     gene            complement(1284..1970)
                     /locus_tag="VLR27_12875"
     CDS             complement(1284..1970)
                     /locus_tag="VLR27_12875"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HSP04395.1"
                     /translation="MDDRTRRRRIHRQALSISLALAPFGIVFGVACVQADLGIAEAVG
                     FSAIVFTGSAQFAAVGVLGDGGGALAAISAGLLLNIRSLAFGVLLAPALTGRVGTRAL
                     LAQLVIDESTAVATVQDEPRWQRYGFLAGGLAVFTVWNLTTIAGVLLASVDDGFITDL
                     GFDAAAPAAFLALLWPRLVGRGGRDGRRIAIAGGLVAAAAVPLAPPGIPILLAGLGVL
                     AARRTREATP"
     gene            2030..2791
                     /locus_tag="VLR27_12880"
     CDS             2030..2791
                     /locus_tag="VLR27_12880"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:NF002198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HSP04396.1"
                     /translation="MTDLLLGSHVAPDDPLGAAAAEGAECVQIFLGDPQSWKKPAPRD
                     DADALKAADVPIYVHAPYLINVASPNNKVRIPSRKILQQTCDAATDIGARAVIVHGGH
                     VTGDDDEADGFVRWRKALDSLETDMPVYLENTAGGDHAMARHFDTIARLWEHIADTGI
                     GFCLDTCHAWAAGEELVEAVERIVGITGRVDLVHCNDSKDESGSGRDRHENLTKGQID
                     PEAILHVVRTAGAPVICETADVGRKDDLAWLRDNL"
     gene            2892..3131
                     /locus_tag="VLR27_12885"
     CDS             2892..3131
                     /locus_tag="VLR27_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04397.1"
                     /translation="MRRRHAIIATTAGAALVGGLVLAPQAMADEHEEEAPASPSLCIN
                     LDISIQGTGEPVSLCLPPEGGEAPGLPELPGLPTP"
     gene            complement(3469..5277)
                     /locus_tag="VLR27_12890"
     CDS             complement(3469..5277)
                     /locus_tag="VLR27_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04398.1"
                     /translation="MSRLEDLLNDAADGPVRFDHRDIARRVRHRRTSRLAMVAAVVVL
                     GVVGFVAGFGEDTQEVDSVGPVEDGAVTVDELVADRWVAFAYSAVVVAPSPPAFLQFG
                     DDGQFGGFDGCRPISGDWELDGGRLVTSLEPMGNVLCDNEPGGLTELLEDDPRVGRFD
                     DGSETLQLTAGDDFVAFQRFDRLGEEPSATALEGRWSASTPAAEGEQEAPAVTFDPDG
                     TGRLEIFGCTQPLKWSLEGDALAVEGLGPDGVPCNGGVVGGGLLMSLTSDPRLRVDGA
                     LLWMSSDLGIDQLHSDGTGSDPSSTTTTTAPDGGQDAEDALERASQAIRFVAASPDGV
                     TLHDGDRPQPISSDDSAVAFAIGSDIVVHQPASTTFPEYPPTPEGTPTAWLSGEEQAL
                     PVNSDARSTRLLYATVLDGTPVALVAESYGGVGPDDTFEELVLIDIADGTRSTVVRRP
                     AWESAHLDAAVLPDGDVIGLFAAESLVLLARWSPGQDDPAWTVEVATDSRPTLTTVDG
                     QIRVVDAGFDEDFAPVLRIRAYDTDGQEQSDRSVPVADPDGELGAGLFCTGWYDATHL
                     LCGRSDGPPIVVSLTSGTYRQTDSATGSFPTVVHDG"
     gene            complement(5274..5735)
                     /locus_tag="VLR27_12895"
     CDS             complement(5274..5735)
                     /locus_tag="VLR27_12895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP04399.1"
                     /translation="MRGRLVGALTLATGRRAVAEELAQEALVRVWQRWDRVSLLDVPE
                     AWAVRVGLNLAGSWHRRRQAEWRANRRADHEEFTVEEDRAAAVAVREAVSALPDRQRS
                     VIVARFFLGYDVAATAALLGCAEGTVKATTHQALARLRTVGLVEDEEVPVR"
     gene            6119..6625
                     /locus_tag="VLR27_12900"
     CDS             6119..6625
                     /locus_tag="VLR27_12900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP04400.1"
                     /translation="MGEMRAEPTERLRTEARDDRWFDALYPHLHRVACVAAPADVDPD
                     DLVQDALVHLLNLDDPAAVRSTKAFLTKTIINLASNERRSWTRRRAAFARLGPTGPNR
                     DQFPSDLTDLLALPPRERAALYLHHVEGLPYDQVAELVGCTPAAARKAAERGRTRLAE
                     TLSKEGTP"
     gene            6622..8484
                     /locus_tag="VLR27_12905"
     CDS             6622..8484
                     /locus_tag="VLR27_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04401.1"
                     /translation="MTLEDSLEQLANRTPPGDPARVLAAARARTESRPEGRRPSLAAA
                     AAVVAVLAVAAAGFAVLNDGDDAVTVAGPDAPGRESQVEVPESGALPQQLADGTPVWV
                     VRHDDGSVSVVDAVSTHEPFGAGTLVAWCESSRGFVDPMYGSLYDEQGGKQAGPAPSG
                     LNTYPVTSVDADTAIVTGTAVEQPRSQDGEPEASEPAGQQCFDTDGDYNAGVIEGSFE
                     SHPLASATPMTPKDAQSETDGTLVVVDAPVVVVKGRQPLVCTSEVAGSPPTCEGIPAP
                     ELYPHDETPVVMRGVFVARPDAGTLTDIAYVGERTVIESDPGPDSGEASAVPGPVLPS
                     FVPDGYRLAWVDTPAEPPVDPERFPLHERPHDYAYGYQTESAELEVRVLVGSTIDDPK
                     AVADEWNALAAPASTAGRTAVGYTDDGGYTRLLVEVDGAVVMVTTHPLGGSFPDGPNP
                     AMPAPDQVDRFAGSIRTVGDDEWAAALGSQGVDVASTFGPKDGTPVVNGDGWALVHGL
                     FRAPLSVRWPSVWVDFGGTPGPAVSLGASEDDVQHAEISIEDDTRVAWGVLPAGAETV
                     EVIIDGRRFDGVVALLDDGTPVFAVDVSGTDGNGTFQALGPTGAPIASGPIEGI"
     gene            8714..9178
                     /locus_tag="VLR27_12910"
     CDS             8714..9178
                     /locus_tag="VLR27_12910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP04402.1"
                     /translation="MRAEVGQELDESLETLYVERYEGLVRLGYLLTGSRGDGEDLAQT
                     AFASLAQSWNEVRSPRSYLRRAVVNRAADLHRRAGRQLPARPEVLVGEPIVDETWDAV
                     QQLSEIQRTVVVLRFYEDLPLVDIAELVGRPEGTVRSDLRRALLRLRKELDR"
     gene            9175..9894
                     /locus_tag="VLR27_12915"
     CDS             9175..9894
                     /locus_tag="VLR27_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04403.1"
                     /translation="MNTDFDTLEAELGDRLRHTLRTIADQPVVHDAPPAERHRWRRAA
                     GITAAALIAVGTAAAFMVQNDDAIVELPADRALMTGEAASGEWWLFPTDAVVDGCPSV
                     TDGVVLVAEESNRPGAELNAGGVVYGEPPTSATACAPHDEQSWLEDPSRADVGHSRLG
                     SERSDTPWGVYGTFHPTIRTVRVHPDGAEPFVVDTVAREDRPDGPRFVAFTLLADTKS
                     AGLDLIDANGATVAVIDRSFG"
     gene            complement(10319..12208)
                     /locus_tag="VLR27_12920"
     CDS             complement(10319..12208)
                     /locus_tag="VLR27_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP04404.1"
                     /translation="MRQGPHSMMPSDASAVDGASLDRSIVRRVMEFARPYKTQIIGFL
                     AAIITAAILGLAPPLLFKWILDDAIADGDKGRLSLLAGLAVAAAVADSGLSLVERWLS
                     SRIGEGLIFDLRVRVFDHVQRLPVAFFTRAQTGALVSRLNNDVIGAQRALTGTLGSVV
                     SNVVVLVTTVAAMVALEWRLTLFTIALLPAFLIPARRVGTRLQAISREQMERNARMNS
                     TMTERFGVAGAMLVKLFGRHDDESDRFADDAAGVRDAGVRSAVLSRVFFSAIALVGAL
                     GIAAVYWWGGILVIDGAITLGTLVALAALVQRIYQPLTALTNARIDIMTAFVSFDRVF
                     EVLDIRNPIEDRPGAQDLVDVQGRVDFDDVWFTYPTAEDVSVPSLAGAVPDHDVSSGP
                     VLRGIDAHVEPGQLVAVVGPSGAGKTTLTSLLPRLYDVDSGAVRIDGHDVRDLTQESL
                     RLAIGVVSQDPHLFHDTIAGNLRYARPDATIDELRTACEAARIHHVIAGLPEGYDTVV
                     GDRGYRMSGGEKQRLSIARMLLKDPAIVILDEATSHLDSENEAAVQDALAEALVGRTS
                     LVIAHRLSTITTADQILVVDDGRVVESGRHDDLVESGGLYADLYRTLVRGSKVPMDIP
                     SLGPY"
     gene            complement(12255..13325)
                     /locus_tag="VLR27_12925"
     CDS             complement(12255..13325)
                     /locus_tag="VLR27_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04405.1"
                     /translation="MDFDIILIGLGAAIVLVVLYDLGATTISLSAVRGPLSGRVASLV
                     WAVGNRSRSERRRLQRITGPLLLVSILIGWFLALTAGWTLIFSAEGALTSTAEPEQQS
                     SRVRWVDALFFVFGRLVGTGSSNLEPDEAAWSTAVALLTLSGVILLTLVIAWILPVVA
                     AVVQKRALASKISALGGSPQGIICHSWTGRDLGDLNLHLLPLIGELTILAQRHLAYPV
                     IHYFHSSDGRTAVGPRIAALDEALTLIAAAGLDDVDRGTGLDVSVTRPLRQAVSDYLS
                     TLEYVFIEPADEPPDPPAFDRLVEEGIVDDEAMRAELAERMEAVRTRRALLRGYVDHD
                     GWTWAAVARDVDAEEEDPEETD"
     gene            complement(13537..13626)
                     /locus_tag="VLR27_12930"
     tRNA            complement(13537..13626)
                     /locus_tag="VLR27_12930"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13590..13592),aa:Ser,seq:cga)
BASE COUNT         2092 a         4803 c         4919 g         1843 t
ORIGIN      
        1 gccgcccacg cggcgtcgtc ggcgaggacg ccggcccggt cggcgacgtc gaccagctcg
       61 tagctggtgc ggacgcgctg cccggagcga ccccagtcca ggaaggtggc ggcggtggtg
      121 acgagggcga ggaggccggc cacccacccc gcgaggtctc gtcgctgcgc cgtcgtcacg
      181 tcacgagcct gctccaccac cgacacgagc gcgccgatcg gttcgttcgt gctgcggtgc
      241 gacacgaacg aaccgaacct cggcgggtca tcggccgtag tcgaccacgc tcggtggtgg
      301 gaggacggcg gctgcgaggt cgtcgggctc ggggtcgccg aacgccgcgc gcacggggag
      361 cacgccggcc cagacctcga ccaggtcggc atcggcgtcg tcgtcgatgg ggccacccgt
      421 gcggatcttg gtggatgcct cgtcgagggc caggcgcacg acgagcgtgc tgcggatctc
      481 gtcgtcgcgc atgggacgga cctccgcggt gcggcccggc acgaggtgct cgacgatggc
      541 gtcgagggca gccgccttct cgtcgtggtc ggtgatgggc tcggcccgac cgaacacgac
      601 gacggaccgg tagttcatcg agtggtggaa cgtgctgcgg gccaggacga gcccgtcgag
      661 cagggtgacg gtgacgcaca ccggctcccc cttgccggtg gcccgcagcc acgtggccgc
      721 cggcgagccg tggatcagca gctcgtcgcc ccggcgggcg taggtggtgg ggatgacgac
      781 cggaccgtgg tcggtgacga gcccgacgtg gcacagcatc ccctcgtcga ggatcgcctc
      841 ggcgacggcg cgatcgtggg tcccgcgatc cgggctgcgc ccgatggtgg tccgctcggt
      901 gcgggggaag gggtcgcgtt cggcggtcac gggtccgcag ccaaggcgac gcgggccgac
      961 cggcgcacat cggtttcagc cggcgacggc ccggacgacc ccggtgaccg ccacggccac
     1021 ggcgaccacc acgatgaacg gcgcccgcag gcgcagggcc acgccgccgg cgaccacgcc
     1081 ggcgaggcgg gcgtcgagca ccagcgcgcg gtcgcccgcc accgtgctca ccaccacgag
     1141 cgcggcgagc agggcggcgg ggagctgctg ggcgatgcgt ccgaccgggg cggggagtcg
     1201 gcggccgccg aggaggagcg gcccggccga cttgagcgcg taggtgccga gcccgaggcc
     1261 gatgatgacg atcagcgggg tgctcatggc gtcgcctcgc gcgtccttcg ggccgcgagg
     1321 acgccgaggc cggcgagcag gatcgggatg cccgggggtg ccagcggcac ggcggcggcg
     1381 gcgacgaggc cgccggcgat ggcgatgcgc cggccgtcgc gcccgccacg cccgacgagc
     1441 cgcggccaga ggagcgcgag gaaggctgcg ggcgcagcgg cgtcgaaccc gaggtcggtg
     1501 atgaacccgt cgtcgacgga ggcgagcagc acgccggcga tggtggtcag gttccacacg
     1561 gtgaacaccg ccagcccgcc ggcgaggaag ccgtagcgct gccagcgggg ctcgtcctgg
     1621 accgttgcca ctgcggtcga ctcgtcgatc acgagctggg ccagcagcgc tcgtgtcccg
     1681 acccgtccgg tgagcgctgg ggcgagcagc accccgaagg ccagggagcg gatgttcagc
     1741 agcagcccgg cgctgatcgc ggcgagggcg ccgccgccgt ccccgagcac accgaccgcc
     1801 gcgaactgcg ccgacccggt gaagacgatc gccgagaacc cgaccgcctc ggcgatcccg
     1861 aggtcggcct ggacgcaggc cacgccgaac acgatcccga agggtgcgag ggccagcgag
     1921 atggagagcg cctggcggtg gatccggcgg cgccgggtgc gatcgtccac gacggtgcat
     1981 cgtgcccgtc gggtcggtgc ccgacccaac ccccactacc gtcggggcca tgacggacct
     2041 gctgctcgga agccacgtcg cccccgatga cccgctcggc gcggctgccg ccgagggtgc
     2101 cgagtgcgtc cagatcttcc tcggcgaccc gcagtcgtgg aagaagccgg cgccccgcga
     2161 cgacgccgac gcgctcaagg ctgccgacgt cccgatctac gtccacgcgc cgtacctgat
     2221 caacgtggcg tcgccgaaca acaaggtccg catcccgagc cgcaagatcc tgcagcagac
     2281 ctgcgacgcc gccaccgaca tcggcgcccg ggcggtcatc gtgcacggcg gccacgtcac
     2341 cggggacgac gacgaggccg acggcttcgt gcgctggcgc aaggccctcg acagcctcga
     2401 gaccgacatg cccgtctacc tggagaacac cgccggcggc gaccacgcca tggcccgcca
     2461 cttcgacacc atcgcccggc tctgggagca catcgccgac accggcatcg gcttctgcct
     2521 cgacacctgc cacgcctggg cggccgggga ggagctggtc gaggcggtcg agcgcatcgt
     2581 cggcatcacc ggacgggtcg acctagtcca ctgcaacgac tccaaggacg agagcgggtc
     2641 cggtcgggac cgccacgaga acctgaccaa gggccagatc gatcccgagg cgatcctcca
     2701 cgtcgtgcgc accgccggcg cccccgtgat ctgcgagacc gccgacgtcg gtcgcaagga
     2761 cgacctggcc tggttgcgcg acaacctctg aggtttcttc gcggcgaccg caaggtcgcc
     2821 ccggctccga cgtttccctg aagacgaccg gcacgccggg tgccggcatc gacaggagga
     2881 caccgaccca catgcgtcga cgccacgcca tcatcgccac caccgccgga gccgctctcg
     2941 tgggcggcct ggtgctcgcc ccgcaggcga tggccgatga gcacgaggag gaggccccgg
     3001 cctccccgag cctctgcatc aacctcgaca tcagcatcca gggcaccggc gagccggtct
     3061 cgctgtgcct gccgcccgag ggtggcgagg cacccgggct gccggagctc cccgggttgc
     3121 ccacgccctg atccgcgcgg gcggggcgat ggaggggccg gggcaggcgg agctccggcc
     3181 cctcgtcgcg tccggggtca ggccgaggga gcgggctcgc ctcgcacgag cgtgctcgtt
     3241 gactcgcccc gaagggtctg ctggccgtcg agccccgtcg tgagccgagc accgtttcga
     3301 cggaccgtca gggcaaccgc gtccggaccg gaccgacccc ggttcggcgt ggactacatg
     3361 ccgcccgtgt cctccaggct gccttcgttc cacatgagtt gtggacggtt ggacgctggg
     3421 tggcgcggat cccgccgcca gcggagcctc cccgacctgc aacgtagctc agccgtcgtg
     3481 gacgactgtt gggaagctgc ctgtcgccga gtcggtctgc cggtaggtgc cggaggtcaa
     3541 cgaaacgacg atgggcggac cgtcgctgcg cccgcagagg aggtgggtgg cgtcgtacca
     3601 gccggtgcag aagagtccgg cgccgagctc gccgtcaggg tcggccaccg ggaccgaccg
     3661 gtcggactgt tcctggccgt cggtgtcgta ggcccggatt cgcagcacgg gggcgaagtc
     3721 ttcgtcgaag cctgcgtcga ccacgcggat ctgcccgtcg acggtcgtga gtgtgggtcg
     3781 gctgtcggtg gcgacctcga cggtccatgc ggggtcgtct tggccggggc tccagcgggc
     3841 gagcagcacg agtgactccg cggcgaagag tccgatgacg tccccgtcgg gtagcacggc
     3901 tgcgtcgagg tgggcggact cccatgccgg tcggcggacc acggtgctgc gggtgccgtc
     3961 cgcgatgtcg atgaggacga gctcctcgaa ggtgtcgtcg ggtccgacgc cgccgtagga
     4021 ctcggcgacg agtgcgaccg gggtcccgtc gaggactgtc gcgtaaagga ggcgggtgct
     4081 gcgagcgtcg gagttgaccg gtagggcctg ctcttcgccg gagagccatg cagttggggt
     4141 gccctcggga gtcggtgggt actcggggaa cgtcgtggat gctggctggt ggaccacgat
     4201 gtcggatccg atggcgaagg cgacggcgga gtcatcgctg ctgatgggct gtggccggtc
     4261 gccgtcgtgg agcgtgactc cgtcgggtga cgcggccacg aaccggatgg cctgtgacgc
     4321 tcgctcgagt gcgtcctcgg cgtcctgtcc gccgtcgggt gcggtggtgg tggtggtgga
     4381 cgacgggtcg gaaccggttc cgtcggagtg gagctggtcg atgccgaggt cagaggacat
     4441 ccacagcaga gctccgtcga cgcgcagtcg ggggtcggac gtcagcgaca tcaggaggcc
     4501 accgccgact acaccgccgt tgcagggcac gccgtcgggg ccgaggccct cgactgccaa
     4561 ggcgtcgcct tcgagcgacc atttcagggg ctgggtgcag ccgaagatct ccaacctgcc
     4621 ggtcccgtcc gggtcgaacg tcaccgccgg ggcttcttgt tcaccttccg ccgccggggt
     4681 ggatgcggac cagcgaccct cgagcgcggt cgcgctcggc tcctcgccca ggcggtcgaa
     4741 ccgctggaac gccacgaagt cgtcgcccgc cgtcagctgc agcgtctccg agccgtcgtc
     4801 gaaccgtccg accctcgggt cgtcctcgag cagctcggtc agcccgccgg gctcgttgtc
     4861 gcacagcacg ttccccatcg gctccagtga cgtgaccagc cggccgccgt cgagctccca
     4921 gtccccgctg atcggtcggc agccgtcgaa cccaccgaac tgaccgtcgt cgccgaactg
     4981 gaggaacgcc ggcggactgg gcgccacgac cacggcgctg taggcgaagg ccacccaccg
     5041 atccgcgacc agctcgtcga ccgtcacggc gccgtcctcg accggaccga cagagtccac
     5101 ctcctgggtg tcttcaccga acccggcgac gaagcccacc acacccagca ccacgaccgc
     5161 cgccaccatt gcgagccggc tggtccggcg gtgccgaacc cggcgagcga tgtcgcggtg
     5221 gtcgaagcgc accggaccgt cggcggcgtc gttcagcaga tcctcgagcc gactcatcga
     5281 accggcacct cctcgtcctc gacgagcccg acggttcgca gccgcgccag ggcctggtgc
     5341 gtcgtcgcct tcacggttcc ctccgcgcaa ccgagcagcg cggctgtcgc cgcgacgtcg
     5401 tagccgagga agaaccgggc cacgatcacc gaccgctgcc ggtcgggcag cgctgagacc
     5461 gcctcacgaa cagccacggc ggcagcccgg tcctcttcga ccgtgaactc ctcgtggtcg
     5521 gctctccggt tcgctcgcca ctccgcctgc cggcggcggt gccacgaccc ggccaggttc
     5581 agccccaccc gcaccgccca cgcttccggt acatccagca gggacacccg gtcccagcgt
     5641 tgccacaccc gcaccagcgc ctcctgggcg agctcctccg cgaccgcgcg ccgcccggtc
     5701 gccagagtga gcgcgccgac cagccggccg cgcacggcgt cgcagaactc ctccggtccc
     5761 tcagctccca tcacgaacaa cacgcatcag cacgccgatc ggtttagccg ggcagaagac
     5821 atccgttggg acatgttctt cccaaggcgc cgacgatcgt tgacctattc atcgctgccc
     5881 tccgctcgcc cgtcggagtg cgggtgagcc gtggcagctc ttcagcccca aacgtggcgt
     5941 cccatcgcga agggcgccaa gcgacgccaa caccacccgg cgacctcggg cctgctcagc
     6001 accaggggag ccgccgccga gggcaccgtg gccgccgatc cgatcgtcac gcgggcggaa
     6061 ctggggcgcc agtctctagg gatggcgatg tcacaaagcc tggctgtgcg gtgtcctcat
     6121 gggtgagatg cgcgccgagc ccacagaacg cctccggacc gaggcccgcg acgacagatg
     6181 gttcgacgcg ctctacccgc acctccaccg cgtggcctgc gtcgccgccc ccgccgacgt
     6241 cgaccccgac gacctcgttc aggacgccct cgtgcacctc ctcaacctcg acgacccagc
     6301 cgccgtccgg tcgaccaagg cgttcctcac gaagacgatc atcaacctcg cgtcgaacga
     6361 acgccggtcg tggacccgcc gtcgtgcagc gttcgcccgc ctcggcccga ccggccccaa
     6421 ccgcgaccag ttcccgtccg acctcaccga cctcctcgcc ctcccaccgc gggaacgggc
     6481 cgccctgtac ctccaccacg tcgaaggact gccgtacgac caggtcgccg agctcgtcgg
     6541 ctgcaccccg gccgcagcac gcaaggccgc cgaacgcggc cgcactcggc tcgccgaaac
     6601 cctctcgaag gagggaaccc cgtgaccctc gaagacagcc ttgaacagct cgccaaccga
     6661 accccacccg gcgaccccgc gagggtgctc gccgctgcac gtgcccgcac cgagtctcga
     6721 ccagagggcc gccgcccctc gctggccgcc gccgcagctg tcgttgccgt gcttgccgtg
     6781 gccgcagccg gcttcgccgt actgaacgac ggggacgacg cagtgaccgt cgccggaccg
     6841 gacgcccccg gcagggagag ccaggtcgaa gtgccggagt cgggggcctt gccgcagcag
     6901 ctcgcggacg gcacgcccgt gtgggtggtc cgtcacgacg acgggtcggt cagcgtggtc
     6961 gacgcggtct cgacccacga gccgttcggg gcaggaactc tcgtcgcttg gtgcgagtcg
     7021 tcgcgcgggt tcgtcgaccc gatgtacggg tcgctgtacg acgagcaagg cggcaagcag
     7081 gccgggcccg cgcccagcgg cttgaacacg tacccggtga ccagcgtgga cgccgacacg
     7141 gcgatcgtga ccgggacggc tgtcgagcag ccacgctcgc aggacggcga accggaggcg
     7201 tccgaacccg ccggtcagca gtgcttcgac accgacggtg actacaacgc cggtgtcatc
     7261 gaagggtcgt tcgagagcca cccgctggcg tcggcgacac ctatgacgcc caaggacgca
     7321 cagagcgaga ccgacggcac cctcgtcgtc gtagacgctc ccgtggtcgt cgtgaaaggc
     7381 aggcagccgc tcgtgtgcac ctccgaggtg gccgggtcac ctccgacctg tgaagggatc
     7441 cccgctcccg agctgtaccc gcacgatgag acgccggtgg tgatgcgcgg cgtgttcgtc
     7501 gcccgcccgg acgcagggac gttgacggac atcgcctacg tcggcgaacg caccgtcatc
     7561 gagagcgatc ccgggccgga ctccggcgag gcgagcgccg ttccggggcc cgtcctgccc
     7621 agcttcgtac cggacgggta ccgcctcgca tgggtcgaca cacccgccga gccgcccgtc
     7681 gaccccgagc gcttcccgtt gcacgaacgg ccgcacgact acgcgtacgg gtaccagaca
     7741 gagagtgccg agttggaggt ccgcgtgctc gtcggctcga caatcgacga cccgaaggct
     7801 gtcgccgacg aatggaacgc gctcgccgca cccgcgtcga cggcgggccg gacggcggtc
     7861 gggtacaccg acgacggcgg ctacacgcgc ttgctggtcg aggtggatgg cgcagtcgtg
     7921 atggtcacca cgcacccgct cggagggtcg ttcccggacg gcccgaaccc ggcgatgcca
     7981 gcaccggacc aagtcgaccg gttcgccggc tccatccgga ccgtcggcga cgacgagtgg
     8041 gcggcggccc tcggctcgca gggcgtggac gtggcttcga cgttcgggcc caaggacggc
     8101 acgcccgtcg tcaacgggga cggatgggcg ctcgtgcacg gcctgttccg cgcccccctg
     8161 tccgtccgct ggccatccgt atgggtggac ttcggtggca cacctggccc ggcagtgagc
     8221 ctcggggcgt ccgaggacga cgtccagcac gcggagatct ccatcgagga cgacacgcgc
     8281 gtggcctggg gagtcctgcc cgccggcgcg gaaacagtcg aagtcatcat cgacggacgg
     8341 cggttcgacg gcgttgtcgc gctcctcgac gacggaacgc cagtgttcgc cgtcgacgtg
     8401 tccggcacgg acggcaacgg gacgttccag gctctggggc caacgggggc cccgatcgcg
     8461 agcggaccga tcgaaggcat ctagccgcga agtcctcgcg tgctcccagg cgggtgaaga
     8521 cgacgacgcc ccagtcgggg tcgcccacct gcgagtccac gcaccgttgg ggaaccgtga
     8581 gcgagtgacc gaccaccgac gcaggcacgc acgggtatca gaatcggcac gtgtgcgcga
     8641 tcgctccgca acgtcgagtc gggtgtcgcg gtgaaggtgc aacgttccga gcgcgctgca
     8701 cgactatgtg gttgtgagag ccgaggtagg acaagaactc gacgagtcgc tcgagacgct
     8761 gtacgtggag cggtacgagg gactggtgcg cctcgggtac ctgctgaccg gcagccgtgg
     8821 cgatggggaa gaccttgccc agaccgcctt cgcatcgctc gcccagtcct ggaacgaggt
     8881 tcgttcccca cgctcctacc tccgccgggc agtcgtgaac cgtgccgccg acctgcaccg
     8941 ccgcgccggc cgacaactac cggcgcgccc cgaagtcctc gtcggtgaac cgatcgtcga
     9001 cgagacatgg gacgcggtgc agcagctctc cgagatccag cgcaccgttg tcgtgttgcg
     9061 cttctacgag gacctgcctc tcgtggacat tgcagaactg gtcggccgtc ctgagggcac
     9121 cgtgcgctcg gacctgcgcc gggccctcct ccgcctacgc aaggagctcg accgatgaac
     9181 accgacttcg acaccctgga ggccgagctc ggcgaccgcc ttcgccacac gctccgaacc
     9241 atcgccgacc agccagtcgt tcacgacgct ccgccggctg agcgccaccg ctggcgtcga
     9301 gctgccggca tcacggctgc tgcgctcatc gctgtcggga cagcggcggc gttcatggtc
     9361 cagaacgacg acgccatcgt cgagctaccc gccgatcggg cgctgatgac cggggaagcg
     9421 gcgtcaggcg aatggtggct cttccccacc gacgccgtcg tcgacggttg cccctccgtg
     9481 acggacggcg tcgttctggt cgccgaggag agcaaccgcc ccggcgccga gctcaacgcc
     9541 ggcggagtcg tctacggcga acccccgacg tctgcaacgg cctgcgctcc ccacgacgag
     9601 cagtcctggc tcgaggaccc gagtcgcgcc gacgtcggac acagccgtct cggctccgag
     9661 cgctccgaca cgccgtgggg cgtctacgga acgttccacc ccaccatccg cacagtgcgg
     9721 gtgcaccccg acggggccga gcccttcgtc gtggacacgg ttgcacgtga agaccgccct
     9781 gacggtccac gatttgttgc cttcaccctt ctggccgaca cgaagtccgc aggcctggac
     9841 ctgatcgacg ccaacggcgc gaccgtcgcg gtcatcgacc gctcgttcgg ctgatcggcc
     9901 acacacgcct ccgggacggg tgcgcaatgg tgccggctcg caaacgcctc tggccacccg
     9961 gtccgggacg aacagggcac cagagaacgc tccttctggt cgcgggccgg tgatgctctc
    10021 ggcgcgacgc tccgtgcaaa ccaggagggc ggactgttgg cgcaaaccag gagggcggac
    10081 ctgttggcct tcagcgcgca accccctgtc cactggcggc aactcaccga ccgctgatgc
    10141 gcgcagggcg gacgcctcga gcacggcggt gttgtgaagc cgagatggcc ggcctctcgg
    10201 acagtgcctg cggcgagacc aacccgtttc ggtcaactgg atcgctgccg agccagcgaa
    10261 ctcgtccttc gcgagcggcg ctcgtcttgg ctgacccttc ctgactgcca gcgggtagtc
    10321 agtacgggcc gagactcggg atgtccatgg ggactttgct cccccgcacg agcgtgcggt
    10381 agaggtcggc gtacaggccg ccggactcca cgaggtcgtc gtggcgtccg gactccacga
    10441 cccggccgtc gtcgacgacc aggatctggt cggcggtggt gatggtggag agccggtggg
    10501 cgatgaccag cgaggtccgt ccgacgagcg cctcggcgag ggcgtcctgc acggcggctt
    10561 cgttctccga gtcgaggtgg ctggtggcct cgtcgaggat gacgatggcc gggtccttga
    10621 gcagcatgcg ggcgatcgac agccgctgct tctcgccgcc ggacatgcgg tagccgcggt
    10681 cgccgacgac ggtgtcgtag ccctcgggca gcccggcgat gacgtggtgg atgcgggccg
    10741 cctcgcaggc ggtgcgcagc tcgtcgatcg tggcgtcggg ccgggcgtag cgcaggttgc
    10801 cggcgatggt gtcgtggaac aggtgggggt cctggctgac gacgccgatg gccagccgca
    10861 ggctctcctg ggtgaggtcc cggacgtcgt ggccgtcgat gcggacggcg ccggagtcca
    10921 cgtcgtagag gcgggggagc agcgaggtga gggtggtctt cccggccccg gacgggccca
    10981 cgacggcgac cagctgcccc ggctcgacgt gcgcgtcgat cccgcgcagc accggcccgg
    11041 acgagacgtc gtggtcgggc acggcaccgg ccagcgacgg gaccgagacg tcctcggcgg
    11101 tcgggtaggt gaaccacacg tcgtcgaagt cgacgcggcc ctgcacgtcg accaggtcct
    11161 gcgcgccggg gcggtcctcg atggggttgc ggatgtcgag gacctcgaac acccggtcga
    11221 aggagacgaa cgccgtcatg atgtcgatgc gggcgttggt cagggcggtg agcggctggt
    11281 agatccgctg caccagggcg gcgagggcga cgagcgtgcc gagggtgatg gcgccgtcga
    11341 tgacgaggat gccgccccac cagtacacgg cggcgatgcc gagcgccccg acgagggcga
    11401 tggcggagaa gaagacccgg ctgaggacgg cgctgcgcac cccggcgtcg cgcaccccgg
    11461 cggcgtcgtc ggcgaagcgg tcagactcgt cgtcgtggcg gccgaacagc ttcacgagca
    11521 tggcgccggc cacgccgaag cgctcggtca tggtcgagtt catccgcgcg ttgcgctcca
    11581 tctgctcgcg gctgatggcc tgcaaccggg tgccgacccg gcgggccggg atgaggaacg
    11641 ccgggagcag ggcgatggtg aacagggtga ggcgccactc gagcgcgacc atggcggcga
    11701 ccgtcgtgac gagcacaacc acgttggaga cgacggagcc gagcgtgccg gtgagggcgc
    11761 gctgggcgcc gatcacgtcg ttgttgagcc gggagacgag cgcaccggtc tgggcccggg
    11821 tgaagaaggc gaccgggagg cgctgcacgt ggtcgaacac ccgcacgcgc aggtcgaaga
    11881 tgagcccttc accgatgcgg gacgacagcc accgctccac gagcgacagg ccggagtcgg
    11941 ccacggcggc ggcgaccgcc agcccggcca ggagggacaa gcgacccttg tcgccgtcgg
    12001 cgatggcgtc gtcgaggatc cacttgaaca gcagcggcgg ggccagaccg aggatcgcag
    12061 cggtgatgat cgcggcgagg aagccgatga tctgggtctt gtacggacgg gcgaactcca
    12121 tgacccgacg cacgatcgag cgatcgaggg acgcgccgtc gacggcggat gcatcgctcg
    12181 gcatcatcga atgggggccc tgtcgcacgg gacgagggta ttggcctcgc cgggttcctc
    12241 ctccggcagc gacgtcagtc ggtctcctcg ggatcctcct cctcggcatc gacgtcgcgg
    12301 gcgaccgcag cccaggtcca cccgtcgtgg tcgacgtagc cccgcagcag ggcgcggcgg
    12361 gtgcgaacgg cctccatgcg ctcggcgagc tcggcgcgca tcgcctcgtc gtcgacgatg
    12421 ccctcctcga ccaggcgatc gaacgcggga gggtccggcg gctcgtccgc gggctcgatg
    12481 aacacgtact ccaacgtgga caggtagtcg ctgacggctt ggcgcagcgg ccgggtgacg
    12541 gacacgtcga ggccggtgcc ccggtcgacg tcgtcgaggc cggcggcggc gatgagggtc
    12601 agggcctcgt cgagcgcggc gatccggggg ccgacggcgg tgcggccgtc ggagctgtgg
    12661 aagtagtgga tcaccgggta cgccaggtgg cgctgggcga ggatggtgag ctccccgatc
    12721 agcggcagca ggtgcaggtt caggtcgccg aggtcgcggc cggtccagct gtggcagatg
    12781 atcccctgcg gcgagccgcc cagcgccgag atcttcgagg cgagcgcccg cttctgcacg
    12841 accgccgcca ccacggggag gatccaggcg atgacgagcg tcaggaggat gacgccggac
    12901 agggtgagca gcgcgaccgc cgtggaccaa gcggcctcgt ccggctcgag gttcgacgaa
    12961 ccggtgccca ccaggcgtcc gaagacgaag aacagcgcat cgacccagcg gacgcgggac
    13021 gactgctgct ccggctcggc ggtgctcgtc agcgcgccct cggcgctgaa gatcagggtc
    13081 cagccggccg tgagggcgag gaaccagccg atgaggatgg acacgaggag cagcggtccg
    13141 gtgatgcgct gcaggcgccg tcgctcgctg cgggaccggt tgcccaccgc ccacaccagc
    13201 gacgcgaccc ggcccgagag cgggccccgg accgcgctga gcgagatcgt ggtggcgccg
    13261 aggtcgtaga gcacgacgag cacgatcgcc gcgccgaggc cgatgaggat gatgtcgaag
    13321 tccactggtt ccgggtgggg tcagctgcgt cgaagggtgc ggcggcgagc gtcgggcact
    13381 cgtcgcagcg agctccctac ccaccggagc gcgggatcga ccggggcggt cgttccgggc
    13441 cgctccacac gcatgggcgg cgcgcctggc tgcccggaac gggggctgct ggccagcgga
    13501 cctcttgcga gagcccgagc tgaccagcag ttccgttggc ggagggagtg ggatttgaac
    13561 ccacggagac ccgaaggcct caacggtttt cgagaccgtc ccattcgtcc gctctggcat
    13621 ccctcctggg agatcaggag ggtagcgaac gggttgc
//
LOCUS       DATISU010000325         1773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_628394, whole genome shotgun sequence.
ACCESSION   DATISU010000325 DATISU010000000
VERSION     DATISU010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628394"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VLR27_12935"
     CDS             complement(<1..191)
                     /locus_tag="VLR27_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP04406.1"
                     /translation="MSDDITERQRQILVEIENHQRERGYPPSVREIGSAVGLTSPSSV
                     HAHLATLQRLGYLQRDPTKP"
     gene            439..849
                     /locus_tag="VLR27_12940"
     CDS             439..849
                     /locus_tag="VLR27_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HSP04407.1"
                     /translation="MVAIASLPTTVTTPGRAHRAPAHRPGPGVRRPALTLVPPVGTGA
                     PSTAGRSLASVVGAALLALAVVLAVGYLATTPTSSPAAGIAGTHVVAEGETMWSIAVA
                     NAPAGEAAGYVERLVEANGGAAVAPGQELLLPSS"
     gene            870..1322
                     /gene="nrdR"
                     /locus_tag="VLR27_12945"
     CDS             870..1322
                     /gene="nrdR"
                     /locus_tag="VLR27_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HSP04408.1"
                     /translation="MRCPSCGDLRDRVVDSRQTEDGRSIRRRRECETCGGRFTTFERV
                     ETALVQVRKRSGGVEGFDASKVAAGVSAACKSRPVDPGQIAVLVADVEDALRSLGKEV
                     TSDEVGLEVLLRLRALDEVAAVRFASVYKGFDTISDFEREIRLLQEPS"
     gene            complement(1332..>1773)
                     /locus_tag="VLR27_12950"
     CDS             complement(1332..>1773)
                     /locus_tag="VLR27_12950"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HSP04409.1"
                     /translation="SADPPRLVVVSGSLDLDLSRAAEGPVRPLVLTTEDADPTRRAAL
                     EELAEVRAYGHGSVELAPALADLRADGVGVVVCEGGPSLNASLVEADLVDEWCLTVAP
                     NVVGGDSRRVVDGAPELVGAGQPLDLVSLLEEDGVLLGRWIVRR"
BASE COUNT          224 a          666 c          635 g          248 t
ORIGIN      
        1 ggcttggtgg ggtcgcgctg caggtagccg aggcgctgga gggtggcgag gtgggcgtgg
       61 accgaggagg gggaggtgag cccgactgcg ctgccgatct cgcgcaccga gggcgggtag
      121 ccccgctctc gctggtggtt ctcgatctcc acgaggatct gacgttggcg ctcggtgatg
      181 tcatcggaca tggggcggcc ctttcggtcg aacaggtgtt tgcaacatac gacaggtccg
      241 cgccgaaggc aaacacccgt tcgtgcaggg tggggacggg tggcgttcgc acgcctgttc
      301 gaccgggcag ttgactcgaa cggtcgttcg tggcaacgta cgaacagctg ttcgcacgaa
      361 cgggcgttct gggtaggcaa gcacctgccg gacccgcccg gcgagttcct gtcagacccc
      421 ctgcggagac tgcacaccat ggtcgccatc gcctccctgc ccaccaccgt caccaccccc
      481 ggccgggccc atcgcgctcc ggcgcaccgg cccggcccgg gggtccgccg cccggcgctc
      541 accctggtgc cgccggtcgg caccggtgcc ccctcgaccg ccggtcgctc cctcgcctcg
      601 gtggtgggtg ccgccctgct cgccctcgcc gtggtgctcg ccgtcggcta cctcgccacc
      661 acgccgacgt cgtcgcccgc cgccggcatc gccggcaccc acgtcgtggc cgagggggag
      721 accatgtggt ccatcgccgt ggccaacgcc cccgccggcg aggccgccgg ctacgtcgag
      781 cgcctcgtcg aggccaacgg cggcgccgcc gtcgccccgg gccaggagct cctcctgccg
      841 tcgagctgat gcccggtagg gtgccgccga tgcggtgccc ctcctgcggc gacctccgag
      901 atcgggtggt cgactcccgc cagaccgagg acggccgttc gatccgccgc cgccgcgagt
      961 gcgagacctg cggcggtcgc ttcaccacct tcgagcgggt cgagaccgcc ctggtccagg
     1021 tccggaagcg ctccggtggc gtcgagggct tcgatgcgtc caaggtcgcg gccggggtga
     1081 gcgcggcgtg caagtcccgt cccgtcgacc cgggccagat cgccgtgctc gtcgccgacg
     1141 tcgaggacgc cctgcgctcg ctcggcaagg aggtcacctc cgacgaggtc ggcctcgagg
     1201 tgctgctgcg gctccgcgcc ctcgacgagg tcgctgccgt gcggttcgcg tccgtctaca
     1261 aggggttcga cacgatctcg gacttcgagc gagagatccg cctcctgcag gagccgtcgt
     1321 aggggtcccg ctcagcgccg aacgatccag cggccgagga ggacgccgtc ctcctcgagc
     1381 agggacacga ggtcgagcgg ctgcccggcg ccgacgagct cgggggcgcc gtccacgacc
     1441 cgccggctgt cacccccgac cacgttcggg gccaccgtca ggcaccactc gtcgaccagg
     1501 tcggcctcga cgagcgacgc gttgagcgac ggcccgccct cgcagaccac gacgcccacc
     1561 ccgtcggcgc gcaggtcggc gagggccggg gccagctcga cgctcccgtg cccgtaggcc
     1621 cggacctcgg ccagctcctc gagcgccgct cggcgggtcg gatcggcatc ctcggtggtc
     1681 agcaccaagg gccgcaccgg cccctccgcg gctcgtgaca ggtcgaggtc gagactcccg
     1741 gagacgacca ccaggcgggg cgggtcggcc gag
//
LOCUS       DATISU010000326         1648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_862238, whole genome shotgun sequence.
ACCESSION   DATISU010000326 DATISU010000000
VERSION     DATISU010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862238"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..635
                     /locus_tag="VLR27_12955"
     CDS             69..635
                     /locus_tag="VLR27_12955"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSP04410.1"
                     /translation="MTETVTIRNGIDVDQLQATIAHVQDDAAAATFTFRASSRWQDGM
                     YNIGRIGSFDHAGETDGSRSEPFTLQGDEPPVLLGDNRGPNAVELVLQALAFCYAAGY
                     VANAAAQGIDITRMDYEIEGDIDLHDFLGQQGPRPGFTEIRATGTVSSPNASEEQLTA
                     LCQYVQDTSPVRDVLANATPVRTTLQVV"
     gene            686..1294
                     /locus_tag="VLR27_12960"
     CDS             686..1294
                     /locus_tag="VLR27_12960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP04411.1"
                     /translation="MLGVDERTELQRLCRQELPGLYALARRLGGVDAEELVQDTLERA
                     CRSFRSLRDPQAACKWLRTILTNIWRDRMRRDGRLPDEVPVEDERDFSLYRTLEEEDP
                     LPYSDTLHVDFLGAFSEHDVHVVLERLPLRYRAPLVLRYIEGFDATEVATLLDLPDGT
                     VMSQLHRGRQRFERELWGYARESGLVDGLATTADGVTEGTTA"
     gene            1291..1560
                     /locus_tag="VLR27_12965"
     CDS             1291..1560
                     /locus_tag="VLR27_12965"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSP04412.1"
                     /translation="MTVHEPIGCHEAVAHLWEMLDGQLDELDQRLLERHLAWCLRCCG
                     ELAFARELRGMLRERSEAAVPDDVLHRLESFIDDLPASTTGEAPG"
BASE COUNT          255 a          593 c          578 g          222 t
ORIGIN      
        1 cgcaatggat cggcggccgt cgtcgtcctg aacaggtgac gggagaagca cgaccagggg
       61 gaggacccat gaccgagacc gtgaccatcc gcaacggcat cgacgtcgac cagctgcagg
      121 cgaccatcgc acacgtccag gacgacgcag ccgccgccac gttcaccttc cgggccagca
      181 gccggtggca ggacggcatg tacaacatcg gccggatcgg ctcgttcgac cacgcaggcg
      241 agaccgacgg ctcccggtcc gaacccttca ccctccaggg cgacgaaccg ccggtgctgc
      301 tcggggacaa ccggggaccg aacgccgtcg agctggtgct ccaggcgctc gcgttctgct
      361 acgccgccgg ctacgtcgcc aacgccgccg cccagggcat cgacatcacc cgcatggact
      421 acgagatcga gggcgacatc gacctgcacg acttcctcgg ccagcagggc cctcgccccg
      481 gcttcaccga gatccgagcg acgggcaccg tgtcgagccc gaacgcctcg gaggagcagc
      541 tgaccgcgct gtgccagtac gtgcaggaca cctcgcccgt gcgcgacgtg ctggccaacg
      601 ccaccccggt gcgcacgacg ctccaggtgg tctgacccct ggacagcgga ggaacggggt
      661 cccacgatgg gggacgggtg attcggtgct cggcgtcgac gagcgaacgg agctgcagcg
      721 gttgtgccgg caggagctgc cggggctcta cgcgctcgcc cgtcggctcg gcggcgtcga
      781 cgccgaggag ctcgtgcagg acaccttgga gcgggcgtgc cgctccttcc gctcgctgcg
      841 cgacccgcag gcggcgtgca agtggctccg cacgatcctc accaacatct ggcgggaccg
      901 catgcgccgg gacggccggc tgcccgacga ggtgccggtc gaggacgagc gcgacttctc
      961 cctctaccgg acgctcgagg aggaggaccc gctgccgtac tccgacacgc tgcacgtcga
     1021 cttcctcggc gcgttctccg agcacgacgt gcacgtggtc ctcgaacggc tcccgcttcg
     1081 ctaccgcgcc ccgctggtgc tgcgatacat cgagggcttc gacgccaccg aggtggcgac
     1141 gctgctcgac ctgccggacg gcacggtgat gtcccagctg caccggggtc ggcagcgctt
     1201 cgagcgcgag ctgtgggggt acgcccggga gtcgggcctc gtcgacggtc tggcgacgac
     1261 ggcggacggg gtgacggagg gaacgacggc atgaccgtgc acgaaccgat cggttgccac
     1321 gaggcggtcg cccacctgtg ggagatgctg gacggccagc tcgacgagct ggaccagcgc
     1381 ctcctcgaac gccacctcgc ctggtgcctg cgctgctgcg gcgagctggc gttcgcccgc
     1441 gagctgcgtg ggatgctgcg cgagcgctcg gaggcggcgg tgcccgacga tgtcctgcac
     1501 cgcctggagt cgttcatcga cgacctgccg gcgtcgacga ccggggaggc accgggatga
     1561 gccacgccac cacgtccccc gacctgctgt tccaggacga gatccgcgct gcggtccgcg
     1621 acgcctaccg ggcgctctcc gccggtgc
//
LOCUS       DATISU010000327         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_721549, whole genome shotgun sequence.
ACCESSION   DATISU010000327 DATISU010000000
VERSION     DATISU010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721549"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VLR27_12970"
     CDS             <1..450
                     /locus_tag="VLR27_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04413.1"
                     /translation="GEEPAPGTDGRACLDGRDDYDGLTSNGCEAEADDLDGTELVDRI
                     AANIVPADDVDRYPMEVADELDLGCNNTLRVEITAPPGLALRLEVLDGQGEVLGQATS
                     ADGIPGEVAIQDPRCFRNDSGTFVAQVTATGSDRTAEPYVLTRRGSF"
     gene            complement(463..777)
                     /locus_tag="VLR27_12975"
     CDS             complement(463..777)
                     /locus_tag="VLR27_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HSP04414.1"
                     /translation="MTPDAITFYWRPGCGFCMGLERKLERLGIPLDKRNIWDDPEASA
                     TVRSIANGNETVPTVVVGEARMVNPRVEHVLAAIRQEAPHLEPDEVPAADGGTLRRFL
                     NR"
     gene            864..1874
                     /locus_tag="VLR27_12980"
     CDS             864..1874
                     /locus_tag="VLR27_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSP04415.1"
                     /translation="MTSPIEHAGFTFASIMALGPQLAIETATRAEQLGYESFWTAETT
                     GPEAFTTLAAAGSAAPSLGLGTGVIPIQLRTPMTVAMAAATLQALHPDRDVLVGIGIS
                     SPVVTERWHGVEYGDRPLARCREYITLLKEIWAGDAVTFEGDFWSLKRSRLGVRLGER
                     KPKVVLGALNPKMLALGGELADGVLLNYLPASHVPWSVERIREGASLRGDGTAPTVYA
                     YVHAGVTEREHGIDNARKDLFSYAVVDAYAKNFERAGFGADVSELRERHAAKDREGAV
                     GAVSDAFVDAIDFMGTPDEVRDFAREYAQAGVDVPILMPLPWGPDRRQVIDDTMAAFV
                     AR"
     gene            complement(1878..2288)
                     /locus_tag="VLR27_12985"
     CDS             complement(1878..2288)
                     /locus_tag="VLR27_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04416.1"
                     /translation="MASERGDESRILLDVRGGDREFVESFLERIGHPVTTCSGPDPGT
                     ICPLLSGEGCTKFQDAHGIVFELDLDRPQHRAILEHYAELRRPELPIRVVVSPEQKVT
                     YAALLADVEVWDHEPTVAELDGFAANVESVDRSR"
     gene            2375..>2755
                     /locus_tag="VLR27_12990"
     CDS             2375..>2755
                     /locus_tag="VLR27_12990"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSP04417.1"
                     /translation="MELQDKNIVVTGAGGGIGAALVRRFAQDGPRAIVLADVDGAAAQ
                     DVVDELEPGFDTVVVEADLGTEAGNVEVVRAAEEYGPVDLLCLNAGIAVGGGVEAPDE
                     DGYRIVDVNVMAPVWAVRAALPGML"
BASE COUNT          389 a         1026 c          966 g          374 t
ORIGIN      
        1 ggcgaggaac ccgccccggg caccgacggg cgggcgtgcc tcgacgggcg ggacgactac
       61 gacgggctca ccagcaacgg ctgcgaggcc gaggccgacg acctggacgg caccgagctg
      121 gtcgaccgca tcgccgccaa catcgtcccg gccgacgacg tcgaccgcta cccgatggag
      181 gtcgccgacg agctcgacct cggctgcaac aacaccctgc gggtggagat caccgcaccg
      241 ccgggcctcg ccctgcgcct cgaggtgctg gacgggcagg gcgaggtcct cggccaggcg
      301 acctcggccg acggcatccc gggcgaggtc gcgatccagg acccgcgctg cttccgcaac
      361 gacagcggca cgttcgtcgc gcaggtgacg gccacgggca gcgatcgcac ggccgagccc
      421 tacgtgctca cccggcgggg cagcttctga tcgacgcctg ggtcagcggt tgaggaaccg
      481 gcgcagcgtg ccgccgtcgg cggccgggac ctcgtcgggc tcgaggtgcg gcgcctcctg
      541 gcggatggcg gcgagcacgt gctcgaccct cgggttgacc atgcgggcct ccccgacgac
      601 cacggtgggc accgtctcgt tgccgttggc gatgctccgg acggtggcgg aagcctcggg
      661 gtcgtcccag atgttgcgct tgtccagggg gatgccgagg cgttcgagct tgcgctcgag
      721 gcccatgcag aagccgcagc cgggccgcca gtagaaggtg atcgcatcgg gggtcatgtc
      781 ggtcacagca cgtacaaccc tgggggccgg accggcattc cacaacctgc ccgacccgga
      841 gcccgcggct acggtccgcg gccatgacga gccccatcga gcacgccggc ttcaccttcg
      901 ccagcatcat ggccctcggc ccgcagctcg cgatcgagac cgccacccgg gccgagcagc
      961 tcggctacga gtcgttctgg accgcggaga ccaccggacc ggaggcgttc accaccctcg
     1021 cggccgccgg ctccgccgcg ccgtcgctcg gcctcggcac cggcgtcatc cccatccagc
     1081 tgcgcacgcc catgacggtc gccatggcgg cggccaccct gcaggcgctg cacccggacc
     1141 gggacgtgct ggtcggcatc gggatctcgt cgcccgtggt gaccgagcgc tggcacggcg
     1201 tcgagtacgg cgaccgcccc ctcgcccgct gccgggagta catcacgctg ctgaaggaga
     1261 tctgggcggg ggacgccgtg accttcgagg gcgacttctg gtccctgaag cgcagccgcc
     1321 tcggcgtgcg cctcggcgag cgcaagccca aggtcgtgct cggtgcgctc aacccgaaga
     1381 tgctggccct cggcggcgag ctggccgacg gcgtgctgct caactacctg cccgcatccc
     1441 acgtcccctg gtcggtcgag cgcatccgtg agggtgcgtc gctccggggc gacggcaccg
     1501 cccccacggt ctacgcctac gtgcacgccg gggtcaccga gcgcgagcac ggcatcgaca
     1561 acgctcgcaa ggacctgttc tcctacgccg tcgtcgacgc ctacgccaag aacttcgagc
     1621 gggccgggtt cggcgccgac gtctccgagc tccgcgagcg ccatgccgcc aaggaccgcg
     1681 aaggtgcggt cggcgccgtc agcgacgcgt tcgtcgacgc catcgacttc atggggaccc
     1741 ccgacgaggt ccgggacttc gcccgggagt acgcccaggc cggcgtcgac gtgcccatcc
     1801 tcatgccgct gccgtggggg cccgatcgcc gtcaggtcat cgacgacacc atggcggcct
     1861 tcgtcgcccg ctgaggctca gcgggagcgg tcgaccgact cgacgttcgc agcgaagccg
     1921 tcgagctcgg ccaccgtcgg ctcgtggtcc cagacctcga cgtccgcgag cagcgccgcg
     1981 taggtgacct tctgctcggg gctgaccacg acgcggatgg gcagctccgg ccgccggagc
     2041 tccgcgtagt gctcgaggat ggcccggtgc tggggccggt cgaggtcgag ctcgaacacg
     2101 atcccgtgcg cgtcctggaa cttcgtgcac ccctcgccgc tgagcagcgg gcagatcgtg
     2161 cccggatccg ggccgctgca ggtggtcacc ggatgtccga tgcgctcgag gaacgactcc
     2221 acgaactcgc gatcaccacc ccgcacgtcg agcaggatcc tggactcgtc tccacgttcg
     2281 cttgccatgc tgaccacccc ctgtggccag tgtccgaggc gggtggcccc ccgcctagag
     2341 gcgttggtca cacgccgtca ctagggtccc gggcgtggag ctccaggaca agaacatcgt
     2401 cgtcaccggc gccggcggcg gcatcggcgc cgccctcgtg cgccgcttcg cccaggacgg
     2461 cccccgcgcc atcgtgctcg ccgacgtcga cggcgccgca gcccaggacg tggtcgacga
     2521 gctcgaaccc ggcttcgaca ccgtcgtggt cgaggccgac ctcggcaccg aggccggcaa
     2581 cgtcgaggtg gtccgggccg ccgaggagta cgggcccgtc gacctgctgt gcctgaacgc
     2641 cggcatcgcc gtcggcggcg gcgtggaggc accggacgag gacgggtacc ggatcgtcga
     2701 cgtcaacgtc atggcccccg tctgggcggt gcgggccgcc ctccccggca tgctc
//
LOCUS       DATISU010000328        22968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_209997, whole genome shotgun sequence.
ACCESSION   DATISU010000328 DATISU010000000
VERSION     DATISU010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 22968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22968
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209997"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="VLR27_12995"
     CDS             <1..1444
                     /locus_tag="VLR27_12995"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSP04418.1"
                     /translation="DGGVTLAQAVHDVAPEVAVVLVAGDVAEGNEVRATLTVTPGIGR
                     HATCVVLHEPDTLDRIADEVERAQLRLQHRRTLERVRQDISAFEGAAPETLSIYLGQL
                     FEHAPVGILLAEPDGVVRAANPCSGEVLGWKPRHAVGTSLADMFSGDAAEIASGLLRE
                     SVGSGGPSEATITRTGPDGTTQHLEVTVAPVDPDHWDLGVFVLLRDESERIQALDAAQ
                     RARQAAEADAKRYAELAWTLQESLLPPDLPAIDGIHLGTRYHPAGDGSEVGGDFYDIF
                     QVADGEWFAVVGDVCGKGAGAARLTALSRYSLRAATVRTKHLGTNLGDLNTALNRQYD
                     LDRARGQHRFATATAIRFEADDGGVTVRAGSGGHPPPLVVRADGTVEEVACRGPLLGV
                     FEEATFTTGETRLGPGDVLVLYTDGVIEARRGREEYGEDRLRDLLSSCAGQRADDVAG
                     AVEEAVLTFQSGVARDDIAVLAIGPEGRPS"
     gene            complement(1437..2120)
                     /locus_tag="VLR27_13000"
     CDS             complement(1437..2120)
                     /locus_tag="VLR27_13000"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HSP04419.1"
                     /translation="MSAAELFRIEPRYDMPSYLWPRPKYRGMLHAIGAFTVVPLGLAL
                     VLDAAGPTARLAAAVYVLAQLIVFATSASYHRLAKSPTARRRMQLADHSMIYLLIAGT
                     WVPICVLVLPEATGRTFLVGVLAAAALGIGLKSFGIDRFPRSSNTMYGVMGGAGLLAL
                     PGIVANISPGAVALLTVGGVAYAVGAFVLLFKRPDPLPSVFGYHEIWHACTVAAAACH
                     FAMVWMIVS"
     gene            complement(2270..2953)
                     /locus_tag="VLR27_13005"
     CDS             complement(2270..2953)
                     /locus_tag="VLR27_13005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSP04420.1"
                     /translation="MRQQPSAPARAVRRRSLAVSAALASVAAAVIASVVLVGSPAQAQ
                     TDVEPAPPVASLAPLFEPVGSLAGSGVDLVAATTTHMITAATPPPPPPPPTHVCPAPG
                     AEFIDSWGFARSGGRRHKGVDMMAPHGTPVYAPAAGNIRASNSSLGGLGFWLEGADGN
                     TYFGSHLQSLSVREGWVEPGTPIGTVGSTGNAGGTPHLHFEVMIGGADSVNPYPFVLD
                     WCGSNDPTG"
     gene            complement(3062..4267)
                     /locus_tag="VLR27_13010"
     CDS             complement(3062..4267)
                     /locus_tag="VLR27_13010"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HSP04421.1"
                     /translation="MPEAVIVATSRTPIGRANKGSLAEARPDDMTAFILKDVLSKVPS
                     LDPTLVEDVMLGCGQPAGEAGYNLARVAAVLAGLDDVPGVTVNRYCSSSLQTIRMAAH
                     AIRAGEGDVFIAAGVEAVSRYMHGASDVGPHHEAFAEAEARTAERSQGGADTWTPPSH
                     LPDIYIAMGQTAENVVQAEGVSRQEMDEWAARSQQRAVESQENGFFEREISSYTLPDG
                     TVMSKDDGPRAGTTVEKLAELKPVFRPDGSVTAGNACPLNDGAAAVVVMSDTKAKELG
                     ITPLARIVSSGVTGLNPEIMGMGPVGAVRQALGRANMTIDDIDLYEINEAFAAQVLPS
                     ARHLGMDLDKLNVKGGSIALGHPFGMTGARIMTTLLNALQDEDKEFGLETMCVGGGQG
                     MAMVVQRLS"
     gene            4316..5653
                     /gene="xseA"
                     /locus_tag="VLR27_13015"
     CDS             4316..5653
                     /gene="xseA"
                     /locus_tag="VLR27_13015"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HSP04422.1"
                     /translation="MADSPDAAPATTTWSVGELSEAIGDRLRAAFPGEVWVRGEIHDL
                     SRARSGHVYFTLRDADQDVTAQLSVMLSSTNKVRVNRLLLRGGGRVRMTDGTEVRIRG
                     RLDWYGPRGQLQLQMTSIDPAFTLGQLAAARAELLERLRGEGLVGANALAPLPVAPLR
                     IGLVTSAGSAAEADVVDELLRSGFAFEITRADVRVQGPEAPGALAGAITGLARTRVDI
                     VLVARGGGAATDLAAFDSELVARAIAAAGKPVITGIGHEVDRSVADEVAHTAAKTPTA
                     AAQHVVGLVAVALERAEHAWQRVAVRATQVLDRADDHLERSRGRAALAARTAAHREQG
                     RIDEAAVRLARSSTSVLDAADRRLDESARRLVSTSRRRVDDAAARLDVLATRADLLDP
                     VHVLSRGWSIARRDDGGIVRSVDDVVAGDALAIRVGDGTLHVRIERVDHDGGP"
     gene            5650..5883
                     /locus_tag="VLR27_13020"
     CDS             5650..5883
                     /locus_tag="VLR27_13020"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF014648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HSP04423.1"
                     /translation="MSETDAPDDQEPAGYGEAVTELESILREIEDVDVDVDVLADRVR
                     RAAQLIEWCRGRILAARTAVEDATADLVDDDHS"
     gene            complement(5986..6468)
                     /locus_tag="VLR27_13025"
     CDS             complement(5986..6468)
                     /locus_tag="VLR27_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04424.1"
                     /translation="MRCRSAATAALLVAACSTGDGLEPLQARVRTPDQRATIDVDGCG
                     RDGDVIVLGASSSSVLVQLLLVLDGDEVDLDASGLTVTLGDRGTLGAGDAEVIQAERP
                     AGAITSANVRGDRIEVVADAEPVTPGADLDAGEVRVVARCTADEELAAPVLDADLTQP
                     "
     gene            complement(6465..7655)
                     /locus_tag="VLR27_13030"
     CDS             complement(6465..7655)
                     /locus_tag="VLR27_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04425.1"
                     /translation="MARGTRIHPLVPALLAVVVVGVLVLVVARSTAGDDEGAAPTTAP
                     TTASTTTEAPTTTTSTMPDPEPVEDLLAPGPAVQAPQAYRITYEIVENGLTRTEEWTV
                     QRPYESLILSRRGDTLLEGTSTSRDKLQTYLADRSGWLVIQPELHRAAFDQHPAAAIG
                     AMEVLGLVEVRGQEEHVGRTCTVYRTGQPPSAGAATAPSEAERTDVCIDDAGLVLHER
                     WEIGGSVVVERTATALELEPAIDPAAFEPGPVVDADDALSRVFSTIAVEADEETLERL
                     ETVVPVPEGYTEDGAVFRASDGGVGAGGASVPGSAEIVRFYSSGPSLLEVAEVFVDGG
                     ADLSSQAAVLVEVDRFGEVWFEPGFRSSSLRARTGESSYVDLRHHDVAFLFDVLRSLQ
                     PADG"
     gene            7739..8812
                     /locus_tag="VLR27_13035"
     CDS             7739..8812
                     /locus_tag="VLR27_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HSP04426.1"
                     /translation="MSQPTRSAVPTPPSLLHVADVVEARLTALLDDEVARWSALTDDL
                     VEPLDALRSLVMAGGKRLRPAFCFWGFVGAGGDPDDPIVVDAGAAFELLQAFALVHDD
                     VMDGSATRRGFRTAHLAFADRHQLEVWRGEGRRFGEGVAILIGDLAHVYADLLLRDAR
                     REVLDVWDELRIELNVGQYLDILGTARGDTDPEGARRIARYKSGKYTIERPLHVGAAL
                     AGRYAELGPALSAYGDPLGEAFQLRDDILGAFGENTVIGKPVGDDLREGKPTPLLAAA
                     SGAADAGQRDVLARIGEPDLTDDEVSAIQTTLRDTGALDEIEALIAQLSASAIAAIQE
                     ADITEEARTALVDLAHFVAWRET"
     gene            8846..10360
                     /gene="crtI"
                     /locus_tag="VLR27_13040"
     CDS             8846..10360
                     /gene="crtI"
                     /locus_tag="VLR27_13040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HSP04427.1"
                     /translation="MRVIVVGAGLGGLSAAAHLRGRGHDVLVLEKAAVPGGRNGLLEL
                     GGYRFDTGPTVFTMPDLLRSAFEALGTEMDDFVTLKPVDPMYRATYHDGTELRVWHGR
                     ERMTQEIRDVVGPEAARQFGRFADWLRELYLLERDHFIDANFDSPLDLAWPLKPALDL
                     VRLGGFGKLGKKVASYFDDHRLQKIFGFQSMYAGLAPYEALAIYCVITYMDSIEGVYF
                     PEGGMHAVPIGLAAALEKAGTELRYGVEVDRIVLAEGTSGPVRGVHVTNPDGTTELLE
                     ADAVVCNPDLPVAYRTLLPGLKQPFNVRHGHYSPSCALWHVGVTGALPERTAHHNIHF
                     GQEWDGAFEDLLKRGTLMGDPSILVTVHSLDEPSLVPDRGDGLDRHSLYVLEPVPNLD
                     GKIDWTRERDRLHDSLQQRLGAMGYPVGAGDIEVEAFLDPTDWERQGMERGTPFALSH
                     KFLQTGPFRPNNVTKHAPGLVFVGSGTLPGVGVPMVLISGKLAAERVDQLDGRR"
     gene            10357..11223
                     /locus_tag="VLR27_13045"
     CDS             10357..11223
                     /locus_tag="VLR27_13045"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HSP04428.1"
                     /translation="MTPAPITLEQSYAHCKALNKRNGTTYYWATTVLPVVKRHHVHAL
                     YGFCRHADDIVDDLGDVATDVRAKALADFGDRFFEDLEAGDSDDLVLKAMVHTVRAFR
                     IDPDCVRRFLRSMAMDLTVETYETWDDLLVYMDGSAAVIGEMMLPILEPTDPAAFAPA
                     RELGNAFQLTNFLRDIDEDLDRSRVYVPQEDIRRFGAESAFATRTATPEFLALMRFEI
                     ERARQLYAVADTGIALLPGRSGRAIRAARTLYARILDQIEAQGLDVFHARARVPTWQK
                     VTIAGRALLLGR"
     gene            11220..11936
                     /locus_tag="VLR27_13050"
     CDS             11220..11936
                     /locus_tag="VLR27_13050"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HSP04429.1"
                     /translation="MTARLAWAPFAVLALTQIGIPLTDDDATLEWLSSLVVVAFFLTV
                     LVIAVARWGAARAVGAAAFVVVATLAVEKLGTATGFPFGEYDYTGALQPTVGGVPVIV
                     PLAWFAMGVTALEVGHHVGSRVVAVVVGALGLTAWDLFLDPQMVEAGYWEWAVDGAYQ
                     GIPLSNYGGWLLTGLVVLAVVEAIRPPTPPSPGLRWLYTWWAVMSTIGFVVFFGRPWV
                     GLVGGIGMGAVTALAWMRAR"
     gene            11938..13389
                     /gene="crtI"
                     /locus_tag="VLR27_13055"
     CDS             11938..13389
                     /gene="crtI"
                     /locus_tag="VLR27_13055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HSP04430.1"
                     /translation="MASVVVVGAGVGGLAASIHLAAAGHDVVVLERLDGAGGKLAEHT
                     AAGFTWETGPSLLTLPEVFRDLAAVAGRSLDDLVTLRRLDPICRYRFPDGTTFDHRAD
                     LDDAAAAAEALSPGSGAEWRAFMAHAREVWEVSERTFFAGAMSSPLELLGRMRSPRDL
                     LAIDPLRSLHARASASFSDPRLVQYVDRYATYSGSSPFRAPATLSCIAHIEQSLGGWY
                     VEGGLGRLADALVELAASAGVEIRTGVDVEAIERRDGAASGVRLAGGEHVAADAVVAN
                     VDALHLYRDLLPHRIRRRRAEAAPRSSSGFVLLLGVEGETPDLTHHNLSFSADYETEF
                     DQLFDDKVPATDPTVYVCAASVTDPAQAPAGHETWFVLVNAPEGGGEMAGYGDHLLDV
                     MAGRGWDLSGRIVHRDQISPADIESRFRTPGGAIYGTSSNGRAAAFLRPGNRGPLDRL
                     YLCGGSSHPGGGLPLVAMSGRIAADLCLGDLVR"
     gene            13482..13916
                     /locus_tag="VLR27_13060"
     CDS             13482..13916
                     /locus_tag="VLR27_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP04431.1"
                     /translation="MTEQPAPLTFQVAIDCREPHVLADWWAEALRWEVEPQDEDFIRS
                     MIEQGHATDDDTDTHRGKLVWRTGAAVVPPEGTTAPRLYFQQVPEAKVAKNRMHLDLR
                     TTDVETERQRLLDLGATELWSGQQGPHTWVTLADPEGNELCI"
     gene            14016..15197
                     /locus_tag="VLR27_13065"
     CDS             14016..15197
                     /locus_tag="VLR27_13065"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HSP04432.1"
                     /translation="MRALTWHGKRDVRVEDVPDPRIEEPTDAIIEITSTGLCGSDLHL
                     YEVLGPYLHAGDVLGHEPMGVVQEVGSAVTNIKAGDRVVIPFQIACGSCFMCDQGLHT
                     QCETTQVTEQGMGAALFGYTDLYGSVPGAQAQYLRVPQAHFGPIVVPDGPSDDRFLFL
                     SDVLPTAWQAVAYADTPKDGTLAVLGLGPIGEMACRIAKHLGVERVIGIDLVPDRLAR
                     AAARGVETIDFRDHKDVAEVVKEMTSGRGADAVIDAVGMEAHGAPVGKLAHQMLAYLP
                     DALGRKIMETAGIDRLGALYPALEMVRRGGTVSLIGVYGGSADPMPMLNMFDKQIQLR
                     MGQANVKRWVDDIMPLLTDDDPLGVDDFATHRVPLEQAPQMYEDFQKKRDGVFKVVFQ
                     P"
     gene            15230..15670
                     /locus_tag="VLR27_13070"
     CDS             15230..15670
                     /locus_tag="VLR27_13070"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSP04433.1"
                     /translation="MSKTARDIMTGNPRIMQMTDCAADIAKVLADENIGAVVICDGER
                     LQGMVTDRDLAVGVLAKGKDPKTCSAADLVDGREVVTIGADDDIDEAIDTMKNHAVRR
                     LPVIDGDKVIGLVAQADIAKHGDDKQVGRLVETISEAPDNSGKG"
     gene            complement(15737..18970)
                     /locus_tag="VLR27_13075"
     CDS             complement(15737..18970)
                     /locus_tag="VLR27_13075"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HSP04434.1"
                     /translation="MATDPVPPRSPLDAFGPRSWARPEQVAVGRLPMRATAYPYPDED
                     LAAGGDRTASPWFHPLDGTWDFALRDRPEAVTPEDVGADAVDDGWLPFEVPGCWTMHP
                     EVPDKPHYTNVRMPFDLEPPEVPDANPTGVHRRTFTLPDGWDGRRAVLHVGGAESCLY
                     VFCNGEPVGMGKDSRLPQEFDLTPFLQAGGNTLALVVVRWSDGSWLEDQDHWWMAGLH
                     REVFLYSTADVHLADVAVTADLDEDLSTGLLQVDVRVGGPLDAGWRLEASVLDDDGAA
                     LLRQPLSADVEVFDRSDPLAESISAYLHRGVTRLRERFDDVSPWSSENPVLHTLVLSL
                     VDPDGNAVESTSVRIGFRRVEVVDRELLLNGYPVLIHGVNRHDHDPDRGKAVTEVGMW
                     DDVVLMKQHNVNAVRTAHYPNDPRFLDACDELGLWVVDEANVESHARLASLSLDPRFT
                     PAILDRIVRTVIRDRNHPSIFGFSLGNESGDAAVFDAAAAWIRRTDPSRVVHYEGACH
                     RAFAGTDDPGDPGPATDLVCPMYPSIDAIRAWAERTADTDEQRPLVMCEFSHAMGNSN
                     GSLADYWAVIETTDGLQGGFIWDWADQGLRATDDAGAEFWAYGGHFGDEPNDATFCCN
                     GLVWPDRSPKPALLEYAYLTAPVRFRASDLKRGRIVVINMQDWRDVSWLGGEWDVRVD
                     GEVVASGTFDLPELPGGFEATVEIDLPTVDVPPGAEAHLGLRCVVTEEQPWAPVGHRV
                     AWQQFDLPETKQHPATGSSAGPTTEPVELDGDVAVAGDVTVRWDPDVAALVDVVLDGR
                     SLLAGPVTASLWRAPTENDGRRLDSTTVPGALAGWLALGLDRVEQEARKVTARRQADG
                     SVQVTLRRRLHLPGLDKAIDHRQRLRVQGDGTVVVDEELALPRSLDDLPRIGARFEVP
                     ASLDLLEWYGRGPLESYADRESSEQLGRWRGRVADQYVPYVLPQEHGHHTDTRWFVLA
                     DGDPAEGGSGVLVSSTDRLEPFGFSARHHHDADLYAATTTAELQMSPTIEVHVDLVNR
                     GLGTGACGPDTLAPYQVTGGPARWSWALRPVRPGDDVARVARRIRTT"
     gene            18995..20083
                     /locus_tag="VLR27_13080"
     CDS             18995..20083
                     /locus_tag="VLR27_13080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSP04435.1"
                     /translation="MPPLPPLRPHLRPGVVVRAALLGTVVARFVRATAAEAPLAAPQP
                     PTDQPDATVVVPARDEQDRIGPCVTSLVGQGAHVIVVDDGSTDRTREVAAAAGATVVD
                     AGPLPDGWAGKAHALQVGLGAASTPVVVFVDADTRAREGFVAAAVDALGDSTLVTAGA
                     RVLVDDAGERWLHPSMLTSLVYRLGPPGVVPSDPARTMANGQCMVVDRERLLDAGGFE
                     PVRGHLLEDLALARSLAGLGHDVRFLDGTAVIDVEGYGSLGATWRGWGRSLDLRTVTP
                     PAAQLLDLAVVWSTMALPLPRLVAGRGDAVDVAALLVRLGTLAGTRGAYARRGLPYVL
                     SPLADLAVAARITAGTVRPSRRWRGRSY"
     gene            complement(20178..20762)
                     /locus_tag="VLR27_13085"
     CDS             complement(20178..20762)
                     /locus_tag="VLR27_13085"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HSP04436.1"
                     /translation="MDQSGAVLAALLTAVVAGYLLGSIPVAVLVGRARGFDPRDVGDR
                     NPGYWNVKEQLGPRSSLPVFVGDVAKGAAAGALGLLLDDPAWGIAHAAVGAAMVGHAY
                     PVFAGFRGGRSILTFVGGMAVLAPVPAAVTVLVLLLVWAATRSFAWAARVGVFGFPIV
                     QLAFESKERVAATGALLSIIGLRFAQASRDRSDP"
     gene            20784..21482
                     /locus_tag="VLR27_13090"
     CDS             20784..21482
                     /locus_tag="VLR27_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04437.1"
                     /translation="MVVASFHLVRHAGALAPTARLPLDRRALRQVPGLRFGRLLGTGE
                     GRTMTTSADLRRWAAFAVWDDIADLDRFRRDHPLSRRWDRAAERFDVQLRPISARGTW
                     DGVDPLAGEDHAGALDGPIAVLTRATVRWRRLPAFLRAVPPVDAHLGSVDGLLQAAGI
                     GEWPIGRQATFSLWRDPEAMQRFSYRSAEHVEVVRRTRDEDWYGEELFARFTPVAWTG
                     TWSGERPLGPDTWA"
     gene            21505..22278
                     /locus_tag="VLR27_13095"
     CDS             21505..22278
                     /locus_tag="VLR27_13095"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HSP04438.1"
                     /translation="MRRALLLGLLTVALIAAGCGDDDDTETTTDTSAATETETDTVTE
                     TETETADPTSTTAAPTPSELPGERIEIFPYEGNELVVVGVEADDVLNVRAAPGVEYEI
                     VAELDPLADGLMPTGHNRQLDDGPIWAEIEVDGETGWASTAFLAHPGAVRDTTSELYP
                     DVADRPRGDTMLQLGELVAERVAGEGEPEPDVVVVDGPTVGDLGEITVDVVGFPDDSV
                     LGERLHIFAEPDPGGESFTVRTVESTTLCRRGVSDGLCV"
     gene            complement(22256..22747)
                     /locus_tag="VLR27_13100"
     CDS             complement(22256..22747)
                     /locus_tag="VLR27_13100"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HSP04439.1"
                     /translation="MTALAVVVAAFVVMEPVTYLAHRYVMHGLGRRLHRSHHRRWPAR
                     ADGEPFFEANDAFPAIFAGAVMVAMAIGFNVDGFSVLLPVTVGVTLYGVAYALVHDGY
                     IHQRLPVRGRHPVLDRLAEAHALHHRFGGEPYGMLVPIVPASVRARAAAADERQLTRT
                     GRR"
     gene            complement(22744..>22968)
                     /locus_tag="VLR27_13105"
     CDS             complement(22744..>22968)
                     /locus_tag="VLR27_13105"
                     /inference="COORDINATES: protein motif:HMM:NF039186.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HSP04440.1"
                     /translation="ISLFFMFLVNGWLTKLSAPIVRYDPDQKTPWRFPWDIPVEDYFF
                     GFSLLTLVLLLWVRQDTPDDPAPWRPEGPA"
BASE COUNT         3156 a         8546 c         8242 g         3024 t
ORIGIN      
        1 cgacggtggg gtcaccctcg cccaggcggt ccacgacgtg gctcccgagg tcgcggtcgt
       61 gctggtggca ggtgacgtcg ccgagggcaa cgaggtgcgg gccacgctca cggtcacgcc
      121 gggcatcggc cgccacgcca cctgcgtcgt cctccacgag cccgacaccc tcgaccggat
      181 cgccgacgag gtcgagcggg cccagctgcg cctccagcac cgccgcacgc tcgagcgcgt
      241 gcgccaggac atctccgcct tcgagggcgc cgcaccggag acgctgtcga tctacctcgg
      301 ccagctgttc gagcacgcgc ccgtcgggat cctgctcgcc gagcccgacg gcgtggtgcg
      361 cgcagccaac ccgtgcagcg gtgaggtgct cggctggaag ccccgccacg cggtcggcac
      421 ctcgctcgcc gacatgttca gcggcgacgc cgccgagatc gcgtccggcc tgctccggga
      481 gagcgtcggc tccggcggtc cgagcgaggc caccatcacc cgcaccggac cggacgggac
      541 cacgcagcac ctcgaggtca cggtcgcgcc ggtcgatccc gaccactggg acctcggcgt
      601 gttcgtgctg ctccgcgacg agtccgagcg catccaggcc ctcgacgccg cgcagcgggc
      661 ccgccaggcg gcggaagccg atgcgaagcg ctacgccgag ctggcctgga ccctccagga
      721 gagcctgctg cccccggacc tgcccgccat cgacgggatc cacctcggca cgcgctacca
      781 cccggccggc gatggctccg aggtcggcgg cgacttctac gacatcttcc aggtggccga
      841 cggcgagtgg ttcgcggtcg tcggcgacgt ctgcggcaag ggtgccggcg cagcgcggct
      901 gaccgccctc agccgctaca gcctgcgggc cgccacggtg cggaccaagc acctcggcac
      961 caacctgggc gacctgaaca cggcgctcaa ccgccagtac gacctcgacc gggcgcgagg
     1021 tcagcaccgc ttcgccaccg ccaccgccat ccgcttcgag gccgatgacg gcggcgtgac
     1081 cgtccgggcc ggatcgggcg ggcacccgcc gcccctcgtc gtccgcgccg acggcacggt
     1141 ggaggaggtg gcgtgccgcg ggccgctcct cggggtcttc gaggaggcca cgttcaccac
     1201 cggcgagacc cgcctcggcc ccggcgacgt gctcgtgctc tacaccgacg gcgtcatcga
     1261 ggcccgccgg ggccgcgagg agtacggcga ggaccgcctc cgggacctcc tgtcgtcgtg
     1321 cgccggccag cgggccgacg acgtggccgg cgccgtcgag gaggccgtac tcacgttcca
     1381 gtcgggcgtg gcccgcgacg acatcgccgt cctcgccatc ggtcccgagg gccgacctag
     1441 ctgacgatca tccagaccat ggcgaagtgg caggcggccg cggcgacggt gcaggcgtgc
     1501 cagatctcgt ggtacccgaa cacgctcggc agcgggtccg ggcgcttgaa cagcagcacg
     1561 aacgccccca ccgcgtaggc gaccccgccg acggtgagca gggccacggc cccggggctg
     1621 atgttggcca cgatcccggg gagggcgagc agcccggcgc cgcccatcac cccgtacatc
     1681 gtgttgctcg accgggggaa gcggtcgatg ccgaaggact tcagcccgat gccgagcgcg
     1741 gcggcggcca gcaccccgac caggaacgtc cgaccggtgg cttcgggcag caccagcacg
     1801 cagatgggga cccacgtgcc ggcgatgagc aggtagatca tcgagtggtc ggccagctgc
     1861 atgcggcgtc gggccgtcgg cgacttggcc aggcggtggt agctggccga ggtcgcgaac
     1921 acgatgagct gggcgagcac gtacaccgcg gcggccagcc gagccgtggg tccggcggcg
     1981 tcgaggacca gcgccagccc cagcgggacc acggtgaagg ccccgatcgc gtgcagcatc
     2041 ccgcggtact tcggacgggg ccacaggtac gacggcatgt cgtaccgggg ctcgatccgg
     2101 aacagctcgg ctgcgctcac cgggaccatc ctcgcgtagc ggggcggccg gaccggaacc
     2161 gcacggtcag gcgggaggtg catcggagcc gagccgacag gcgaggcgac ccggatgacg
     2221 ggccctgtgg cggcgaagcc gtcacgatcg aacagcagcg ggacgtgacc tatccggtcg
     2281 gatcgttcga gccgcaccag tcgaggacga acgggtacgg gttgaccgag tcggccccgc
     2341 cgatcatgac ctcgaagtga aggtgcggcg tgccgccggc gttgccggtg gacccgaccg
     2401 tgccgatggg cgtccccggc tcgacccagc cctcgcggac gctgagggac tgcaggtgcg
     2461 acccgaagta ggtgttgcca tcggcaccct cgagccagaa gccgagaccg ccgagggacg
     2521 agttcgacgc ccggatgttc ccggccgccg gggcgtacac gggcgtgccg tggggcgcca
     2581 tcatgtcgac gcccttgtgc cggcggccgc cggagcgggc aaagccccac gagtcgatga
     2641 actccgcgcc cggcgccggg cacacgtgcg tgggcggcgg gggcggaggc ggcggggtcg
     2701 ccgcggtgat catgtgggtc gtggtggccg cgacgaggtc gacgccgctg ccggcgaggc
     2761 tgccgacggg ctcgaacagc ggggccagcg acgccacggg cggtgcgggc tccacgtcgg
     2821 tctgggcctg ggcgggtgac ccgaccagga ccacggaggc gatcacggcg gcggcgacgc
     2881 tggcgagggc ggcggagacc gcgagcgaac ggcgtcggac agcccgcgca ggggccgagg
     2941 gctgctggcg catccggaga tgctttcatt acggaaaggt gacggtcaaa ccgagaccgt
     3001 cacctctccg taaccagaac tcgttctggt cagacccccg agggggaatg accgccccgg
     3061 ctcagctgag gcgctggacc accatggcca tgccctggcc gccgccgacg cacatcgtct
     3121 cgaggccgaa ctccttgtcc tcgtcctgga gggcgttgag gagcgtggtc atgatgcggg
     3181 cgccggtcat gccgaacggg tgaccgaggg cgatcgagcc gcccttgacg ttgagcttgt
     3241 cgaggtccat gccgaggtgc cgggccgagg gcagcacctg ggcggcgaag gcctcgttga
     3301 tctcgtacag gtcgatgtcg tcgatggtca tgttcgcccg gcccagggcc tggcggacgg
     3361 cgccgaccgg gcccatgccc atgatctcgg ggttcaggcc ggtgacgccc gaggacacga
     3421 tgcgggccag cggggtgatg cccagctcct tggccttggt gtcgctcatg acgacgacgg
     3481 cggcggcgcc gtcgttgagc gggcaggcgt tgccggcggt gaccgagccg tcggggcgga
     3541 acaccggctt cagctcggcc agcttctcga ccgtggtgcc ggcccggggg ccgtcgtcct
     3601 tggacatgac cgtgccgtcg gggagggtgt aggagctgat ctcgcgctcg aagaagccgt
     3661 tctcctggct ctcgaccgca cgctgctggg aacgggcggc ccactcgtcc atctcctggc
     3721 gggacacgcc ctcggcctgg accacgttct cggcggtctg gcccatggcg atgtagatgt
     3781 cgggcaggtg cgacgggggc gtccacgtgt cggcgccgcc ctgggagcgc tcggcggtgc
     3841 gggcctcggc ctcggcgaag gcctcgtggt gcgggccgac gtcggacgcg ccgtgcatgt
     3901 agcgggacac ggcctcgacg ccggcggcga tgaagacgtc gccctcgccg gcgcggatgg
     3961 cgtgggcggc catgcggatg gtctgcagcg acgaggagca gtagcggttg acggtgacgc
     4021 ccgggacgtc gtcgaggccg gcgagcacgg cggcgacgcg ggccaggttg tacccggcct
     4081 cgccggcggg ctgcccgcag ccgagcatga cgtcctcgac cagggtgggg tcgagcgacg
     4141 gcaccttgga cagcacgtcc ttcaggatga acgccgtcat gtcgtcgggg cgggcctcgg
     4201 cgagggaccc cttgttggct cgaccaatgg gcgtgcggga cgtggcgacg atgactgctt
     4261 cgggcatggg gccctcctcg gctcgggaac ggccgccgac agtaccgtcg gaaccgtggc
     4321 cgactcaccc gacgccgcgc cggcgaccac gacctggtcg gtcggcgagc tgtccgaggc
     4381 catcggcgac cggctgcggg ccgcgttccc cggcgaggtc tgggtgcggg gcgagatcca
     4441 cgacctcagc cgggcccggt cgggccacgt gtacttcacc ctgcgcgacg ccgaccagga
     4501 cgtcaccgcc cagctctcgg tgatgctgtc ctcgaccaac aaggtgcggg tcaaccggct
     4561 gctgctccgc gggggcgggc gggtgcggat gaccgacggc accgaggtgc ggatccgggg
     4621 ccggctcgac tggtacggcc cccggggcca gctgcagctg cagatgacct cgatcgaccc
     4681 ggccttcacg ctcggccagc tcgccgcggc ccgggccgag ctgctcgagc ggctccgggg
     4741 cgaggggctg gtcggggcca acgccctggc accgctgccg gtcgcaccgc tgcggatcgg
     4801 gctggtgacg tcggcgggca gcgcggcgga ggccgacgtg gtcgacgagc tgctccgatc
     4861 ggggttcgcc ttcgagatca cccgcgccga cgtccgggtc cagggccccg aggcgccggg
     4921 cgcgctggcc ggggccatca ccggcctggc ccgcacgcgg gtcgacatcg tcctggtggc
     4981 gcggggcggc ggtgcggcca ccgacctggc tgccttcgac agcgagctgg tcgcccgcgc
     5041 catcgcggcc gccgggaagc cggtcatcac cgggatcggc cacgaggtcg accgctccgt
     5101 cgccgacgag gtcgcccaca ccgcggccaa gacgcccacg gccgccgccc agcacgtcgt
     5161 cgggctcgtg gccgtcgcgc tggagcgagc cgagcacgcc tggcagcggg tcgcggtccg
     5221 ggcgacccag gtgctcgacc gggccgacga ccacctggag cgctcccggg ggcgggccgc
     5281 gctcgccgcc cgcaccgccg cccaccgcga gcaggggcgc atcgacgagg ccgccgtccg
     5341 cctggcgcgg agctcgacgt cggtgctcga cgccgccgac cggcggctcg acgagtccgc
     5401 ccgccgcctc gtgtccacct cccgccgccg cgtcgacgac gccgccgccc gcctggacgt
     5461 gctggcgacc cgcgccgacc tgctcgaccc cgtgcacgtg ctgtcgcggg ggtggtccat
     5521 cgcccgccgg gacgacggcg gcatcgtccg gtccgtcgac gacgtcgtgg ccggggacgc
     5581 cctcgccatc cgggtgggcg acggcaccct ccacgtgcgg atcgagcgcg tcgaccacga
     5641 tggcgggcca tgagcgagac cgacgcccct gacgaccagg agcccgccgg ctacggcgag
     5701 gcggtcaccg agctcgagtc gatcctgcgc gagatcgagg acgtcgacgt ggacgtcgac
     5761 gtcctggccg accgggtccg ccgggccgcg cagctcatcg agtggtgccg aggccgcatc
     5821 ctggcggccc gcaccgccgt ggaggacgcc accgccgacc tcgtcgacga cgaccactcc
     5881 tgaaactggc agtcgcggcg tcgcggcggt gcccgggcac gcgccgccac ccgaactgga
     5941 cgcgatgccg acgcatcggc tgcgtcagat ctgggtccag aacggtcagg gctgcgtcag
     6001 atccgcgtcc agaaccggag cggcgagctc ctcgtcggcg gtgcagcggg ccacgacccg
     6061 cacttcaccg gcgtccaggt cagcaccggg cgtgaccggt tcggcgtcgg cgacgacctc
     6121 gatgcgatcg cctcggacgt tggcggacgt gatggcgccg gcgggacgct cggcctggat
     6181 gacctcggcg tcgccggcgc cgagcgtgcc ccggtccccg agggtcacgg tgagcccgga
     6241 cgcgtcgagg tcgacctcgt cgccgtcgag caccagcagc agctgcacca ggacggagga
     6301 cgacgacgcg ccgaggacga tcacgtcgcc atcccgaccg cagccgtcca catcgatggt
     6361 cgcccgctgg tccggggtgc gcacccgggc ctgcagcggt tccaagccgt cgccggtcga
     6421 gcacgccgcg acgaggaggg cggccgtcgc cgccgaccgg cacctcatcc gtcagcgggc
     6481 tgcagggagc ggaggacgtc gaacaggaac gccacgtcgt ggtggcgcag gtccacgtag
     6541 gaggactcgc cggtgcgggc gcgcagcgag gagctgcgga agcccggctc gaaccagacc
     6601 tcgccgaacc tgtcgacctc caccaggacc gccgcctgcg acgacaggtc ggcgccgccg
     6661 tcgacgaaca cctcggcgac ctcgagcagc gacgggcccg acgagtagaa gcgcacgatc
     6721 tccgcgctgc cggggacgga cgcgccgccg gcaccgaccc cgccgtccga ggcccggaag
     6781 acggcgccgt cctcggtgta gccctcgggc actgggacca cggtctcgag gcgctcgagg
     6841 gtctcctcgt cggcctcgac cgcgatggtg gagaacaccc gggacagcgc atcgtcggcg
     6901 tcgaccaccg ggccgggctc gaaggcggcc gggtcgatgg cgggctcgag ctcgagggcg
     6961 gtggcggtgc gctcgacgac gacgctgccc ccgatctccc agcgctcgtg gagcacgagc
     7021 ccggcgtcgt cgatgcacac atcggtccgc tcggcctcgc tcggcgccgt ggccgcaccg
     7081 gcgctcggcg gctggccggt gcggtagacg gtgcacgtcc gccccacgtg ctcctcctgg
     7141 ccacggacct ccaccaggcc gagcacctcc atcgccccga tggcggcggc cgggtgctgg
     7201 tcgaaggcgg cccggtgcag ctcgggctgg atcaccagcc acccggagcg gtcggcgagg
     7261 taggtctgca gcttgtcccg ggacgtcgac gtcccctcca gcagggtgtc gccccgccgc
     7321 gacaggatca gggactcgta cgggcgctgg accgtccact cctcggtgcg ggtcagcccg
     7381 ttctccacga tctcgtaggt gatgcggtac gcctgcggcg cctgcacggc gggcccgggg
     7441 gcgagcaggt cctcgaccgg ctccgggtcg ggcatcgtcg aggtggtcgt ggtcggggcc
     7501 tcggtggtgg tgctcgccgt cgtgggtgcc gtcgtcggcg ccgcgccctc gtcgtcgccg
     7561 gcggtcgagc gcgccacgac cagcaccagc acgccgacga cgacgaccgc gagcagagcc
     7621 ggcaccagcg ggtggatgcg ggtcccccga gccatggcgg tcagcatgcc agccgagctg
     7681 gaggtggccc gtaccgcgag ggcaagggtg acgagcagcg ccgtaggctc ggggcaccat
     7741 gtcgcaaccg acccgcagcg ccgtcccgac gccgccgagc ctcctgcacg tcgccgatgt
     7801 cgtcgaggcc cggctcaccg cgctgctcga cgacgaggtg gcccgctggt ccgcgctgac
     7861 cgacgacctc gtcgagcccc tcgacgccct gcgctccctc gtcatggccg gcggcaagcg
     7921 cctccggccc gccttctgct tctggggctt cgtcggcgcc gggggcgacc ccgacgatcc
     7981 catcgtcgtc gatgccggcg ccgccttcga gctcctgcag gccttcgccc tggtgcacga
     8041 cgacgtgatg gacggctccg ccacccgacg cgggttccgc accgcccacc tggccttcgc
     8101 cgaccgccac cagctcgagg tgtggcgcgg ggagggccgt cgcttcggcg agggcgtggc
     8161 catcctcatc ggcgacctgg cccacgtcta cgccgacctg ctgctgcgcg acgcccgtcg
     8221 cgaggtcctc gacgtctggg acgagctgcg catcgagctc aacgtcggcc agtacctcga
     8281 catcctcggc acggcccggg gcgacaccga tcccgagggc gcccgccgga tcgcccggta
     8341 caagtccggc aagtacacga tcgagcggcc cctgcacgtg ggggcggccc tggccggccg
     8401 gtacgccgag ctcggcccgg cgctgtcggc ctacggcgac cccctcggcg aggccttcca
     8461 gctgcgcgac gacatcctcg gcgcgttcgg cgagaacacg gtcatcggca agcccgtggg
     8521 cgacgacctg cgcgagggca agcccacccc gctgctggcc gcggccagcg gcgccgccga
     8581 cgccggccag cgcgacgtgc tggctcgcat cggcgagccc gacctcaccg acgacgaggt
     8641 gtcggcgatc cagacgacgc tgcgcgacac gggggcgctc gacgagatcg aggccctcat
     8701 cgcccagctc tccgccagcg ccatcgccgc catccaggag gctgacatca ccgaggaggc
     8761 ccggaccgcg ctcgtggacc tggcccactt cgtcgcctgg cgcgagacat gacatccaga
     8821 cgccgatcgg ctccggagac ctgacgtgcg cgtgatcgtc gtcggtgccg gactcggtgg
     8881 cctgtcggcc gcagcccacc tgcgcgggcg cggccacgac gtcctggtgc tggagaaggc
     8941 tgccgtcccc ggcggtcgca acggcctgct cgagctcggc ggctaccgct tcgacaccgg
     9001 gcccaccgtg ttcaccatgc cggatctgct ccgctcggcc ttcgaggcgc tcggcaccga
     9061 gatggacgac ttcgtcacgc tgaagccggt cgacccgatg taccgggcca cctaccacga
     9121 cggcaccgag ctccgggtct ggcacggccg cgagcgcatg acccaggaga tccgcgacgt
     9181 cgtcgggccc gaggccgccc ggcagttcgg ccgcttcgcc gactggctgc gcgagctgta
     9241 cctgctcgag cgcgaccact tcatcgacgc caacttcgac tcgcccctcg acctggcgtg
     9301 gccgctgaag ccagcactcg acctggtccg cctcggcggc ttcggcaagc tcggcaagaa
     9361 ggtggcgtcg tacttcgacg accaccggtt gcagaagatc ttcggcttcc agtccatgta
     9421 cgccggcctc gctccctacg aggcgctggc catctactgc gtcatcacct acatggactc
     9481 catcgagggc gtgtacttcc ccgagggcgg catgcacgcc gtgccgatcg ggctcgccgc
     9541 tgcgctcgag aaggccggca ccgagctgcg ctacggcgtc gaggtcgacc gcatcgtcct
     9601 ggccgagggc accagcgggc cggtccgggg cgtgcacgtc accaaccccg acggcaccac
     9661 cgagctgctc gaggccgacg ccgtcgtctg caaccccgac ctccccgtcg cctaccgcac
     9721 cctgctgccc ggcctgaagc agccgttcaa cgtgcgccac ggccactact cccccagctg
     9781 cgccctctgg cacgtggggg tgacgggagc gctgccggag cggacggcgc accacaacat
     9841 ccacttcggc caggagtggg acggcgcctt cgaggacctg ctgaagcggg gaaccctgat
     9901 gggcgacccg tcgatcctcg tcaccgtgca cagcctcgac gagccgtccc tggtccccga
     9961 ccggggcgac gggctcgacc gccactcgct gtacgtgctc gaacccgtcc cgaacctcga
    10021 cgggaagatc gactggaccc gggagcggga tcgcctgcac gactcgctcc agcagcgcct
    10081 cggcgccatg ggctacccgg tcggcgccgg cgacatcgag gtcgaggcct tcctcgaccc
    10141 caccgactgg gagcgacagg gcatggagcg gggcaccccg ttcgccctgt cccacaagtt
    10201 cctccagacc ggcccgttcc ggcccaacaa cgtcaccaag cacgcccccg ggctcgtctt
    10261 cgtcggttcc ggcacgctgc ccggcgtcgg ggtcccgatg gtgctgatct cgggcaagct
    10321 cgccgccgag cgcgtcgacc agctggacgg ccgacgatga ccccggcgcc gatcacgctc
    10381 gagcagagct acgcccactg caaggcgctc aacaagcgca acggcaccac ctactactgg
    10441 gccaccacgg tgctgccggt cgtgaagcgg caccacgtgc acgccctcta cgggttctgc
    10501 cgccacgccg acgacatcgt cgacgacctc ggcgacgtcg ccaccgacgt gcgggccaag
    10561 gcgctggccg acttcggcga ccgcttcttc gaggacctcg aggccggcga cagcgacgac
    10621 ctcgtgctca aggccatggt ccacaccgtg cgggcgttcc gcatcgaccc tgactgcgtg
    10681 cgccggttcc tgcgctcgat ggcgatggac ctcaccgtcg agacctacga gacctgggac
    10741 gacctgctcg tctacatgga cggctcggcc gcggtgatcg gcgagatgat gctgccgatc
    10801 ctggagccga ccgacccggc cgcgttcgca ccggcacggg agctgggcaa cgcgttccag
    10861 ctcaccaact tcctccggga catcgacgag gacctcgacc gctcccgcgt ctacgtgccc
    10921 caggaggaca tccgccgctt cggcgccgag tccgccttcg cgacccggac ggcgaccccc
    10981 gagttcctgg cgctgatgcg cttcgagatc gagcgggccc ggcagctgta cgccgtggcc
    11041 gacaccggca tcgccctgct gcccggccgc tccggtcgcg ccatccgcgc cgcccgcacc
    11101 ctctacgccc gcatcctcga ccagatcgag gcccaggggc tcgacgtgtt ccacgcgcgt
    11161 gccagggtgc cgacgtggca gaaggtgacc atcgcgggac gggcgctgct cctcggtcga
    11221 tgaccgcccg cctggcgtgg gcgccgttcg ccgtgctggc gctcacccag atcggcatcc
    11281 ccctcaccga cgacgacgcc accctcgagt ggctctcgtc gctggtggtc gtcgccttct
    11341 tcctcaccgt cctcgtcatc gccgtcgccc gctggggcgc agcccgcgcc gtcggagccg
    11401 ccgcgttcgt ggtggtcgcc accctggccg tcgagaagct cggcaccgcc accggcttcc
    11461 ccttcgggga gtacgactac accggcgcgc tccaaccgac ggtcggcggc gtgccggtga
    11521 tcgtcccgct ggcgtggttc gccatggggg tcaccgcgct ggaggtcggg caccacgtcg
    11581 gctcccgggt cgtggcggtc gtcgtcggtg cgctcgggct gacggcgtgg gacctgttcc
    11641 tcgaccccca gatggtggag gccggctact gggagtgggc cgtcgacggc gcctaccagg
    11701 gcatcccgct gtcgaactac gggggctggc tgctcaccgg gctggtcgtg ctggccgtcg
    11761 tcgaggccat ccgcccgccc acgccgccgt cgccggggct gcggtggctg tacacgtggt
    11821 gggcggtcat gagcaccatc ggcttcgtcg tgttcttcgg ccggccctgg gtcggcctgg
    11881 tcggcggcat cggcatgggc gcggtcaccg ccctggcctg gatgcgggcg cgctgacgtg
    11941 gcctcggtcg tggtcgtcgg cgccggtgtg gggggcctcg ccgcgtcgat ccacctcgcc
    12001 gcggccggcc acgacgtggt ggtcctcgag cgcctggacg gcgccggcgg gaagctggcc
    12061 gagcacactg cggccgggtt cacgtgggag accggaccgt cgctgctgac gctgcccgag
    12121 gtgttccgcg acctggccgc cgtcgccggg cggtcgctgg acgacctcgt caccctccgc
    12181 cggctggatc cgatctgtcg ctaccgcttc cccgacggca ccacgttcga ccaccgtgcg
    12241 gacctcgacg acgcggccgc agccgccgag gcgctctcgc cgggcagcgg tgccgagtgg
    12301 cgggcgttca tggcccacgc ccgcgaggtg tgggaggtgt ccgagcggac gttcttcgcc
    12361 ggcgccatgt cctccccgct ggagctgctc ggccggatgc ggtcgccccg cgacctgctg
    12421 gccatcgacc cgctccgctc gctccacgcc cgcgcatcgg cgtcgttctc cgacccccgc
    12481 ctggtgcagt acgtcgaccg ctacgccacc tactcggggt cgtccccgtt ccgggcgccg
    12541 gcgacgctga gctgcatcgc ccacatcgag cagtcgctgg gcggttggta cgtcgagggt
    12601 gggctcggcc ggctcgccga cgcgctggtc gagctggccg ccagcgccgg cgtggagatc
    12661 cgcaccggcg tcgacgtcga ggcgatcgag cggcgggacg gcgcggccag cggggtccgc
    12721 ctggccggcg gggagcacgt ggccgccgac gcggtcgtcg ccaacgtcga cgccctccac
    12781 ctgtaccgcg atctgctccc ccaccgcatc cgccggcggc gggccgaggc cgcaccgcgc
    12841 tcgtcctcgg gcttcgtgct gctgctcggc gtcgagggcg agaccccgga cctcacccac
    12901 cacaacctgt cgttctccgc cgactacgag accgagttcg accagctctt cgacgacaag
    12961 gtgcccgcca ccgaccccac cgtctacgtc tgcgccgcgt ccgtcaccga cccggcccag
    13021 gcgccggccg ggcacgagac ctggttcgtg ctcgtgaacg cgcccgaggg cggcggcgag
    13081 atggccggct acggcgacca cctgctcgac gtgatggcgg ggcggggctg ggacctgtcg
    13141 ggtcgcatcg tccaccgcga ccagatcagc ccggccgaca tcgagtcccg cttccgcacc
    13201 ccgggcggcg cgatctacgg cacgtcgtcc aacggccggg ccgcggcgtt cctccgcccg
    13261 ggcaaccgcg gtcccctcga ccggctctac ctgtgcggcg gcagcagcca ccccggcggc
    13321 ggcctgcccc tcgtggcgat gagcggccgc atcgccgccg acctgtgcct gggggacctc
    13381 gtacgctgag tcccgggcaa gcccctcgtt ctgggccgcg gccgacgcat ccacggcgtg
    13441 tggagcggcc cggtcccacc ttcagcaccg gaggccgcat cgtgaccgaa cagcccgcac
    13501 ccctgacctt ccaggtcgcc atcgactgcc gggagcccca cgtcctcgcc gactggtggg
    13561 ccgaggccct gcgctgggag gtggagcccc aggacgagga cttcatccgc tccatgatcg
    13621 agcagggcca cgccaccgac gacgacaccg acacccaccg gggcaagctc gtgtggcgca
    13681 ccggggccgc cgtcgtgcca cccgaaggca ccacggcgcc gcgcctgtac ttccagcagg
    13741 tgccggaggc aaaggtcgcc aagaaccgca tgcatctcga cctgcgcacg accgacgtgg
    13801 agaccgagcg gcagcggctg ctcgacctcg gcgccaccga gctgtggtcc ggtcagcagg
    13861 gcccgcacac gtgggtgacg ctggccgacc ccgagggcaa cgagctctgc atctgacgtc
    13921 gcagccgcga cagcagcact gccagttccg ggagatgcgc atggcacccc ctggcccgcg
    13981 ggtagcgacc tcctccggac ccgatggagg aacacatgcg cgcactgacc tggcacggca
    14041 agcgagacgt ccgagtcgag gacgtccccg atccgaggat cgaggagccg accgacgcca
    14101 tcatcgagat cacctccacc ggcctgtgcg gctctgacct gcacctctac gaggtgctcg
    14161 gcccgtacct gcacgccggc gacgtcctcg gccacgagcc gatgggcgtc gtccaggagg
    14221 tcggctccgc ggtcacgaac atcaaggccg gtgaccgggt cgtcatcccg ttccagatcg
    14281 cctgcggctc ctgcttcatg tgcgaccagg gcctgcacac ccagtgcgag accacccagg
    14341 tcaccgagca gggcatgggc gccgccctct tcggctacac cgacctctac ggcagcgtcc
    14401 cgggcgcgca ggcccagtac ctgcgggtcc cccaggccca cttcggcccg atcgtcgtgc
    14461 ccgatggccc gtcggacgac cgcttcctct tcctctccga cgtgctgccc accgcctggc
    14521 aggccgtcgc gtacgccgac accccgaagg acggcaccct cgccgtgctg ggcctcggcc
    14581 ccatcggtga gatggcgtgc cggatcgcca agcacctcgg ggtcgagcgg gtgatcggca
    14641 tcgacctcgt ccccgaccgc ctcgctcgcg ccgcagcgcg cggcgtggag accatcgact
    14701 tccgggacca caaggacgtc gccgaggtcg tgaaggagat gacgtccggt cgcggtgcgg
    14761 atgccgtcat cgacgccgtc ggcatggagg cccacggcgc acccgtgggc aagctcgccc
    14821 accagatgct ggcctacctc cccgacgccc tcggccggaa gatcatggag accgccggca
    14881 tcgatcgcct cggcgcgctg taccccgccc tcgagatggt ccgccgtggc ggcaccgtgt
    14941 cgctcatcgg cgtgtacggc ggttcggccg acccgatgcc gatgctgaac atgttcgaca
    15001 agcagatcca gctgcgcatg gggcaggcca acgtgaagcg ctgggtcgac gacatcatgc
    15061 cgctgctcac cgacgacgac ccgctcggcg tggacgactt cgccacccac cgtgtccccc
    15121 tcgagcaggc cccccagatg tacgaggact tccagaagaa gcgcgacggc gtcttcaagg
    15181 tcgtcttcca gccctgatca gcaccgcacc cagaccacag gagaccacca tgagcaagac
    15241 cgcacgagac atcatgacgg gcaacccccg catcatgcag atgaccgact gcgccgccga
    15301 catcgccaag gtgctcgccg acgagaacat cggcgccgtc gtcatctgcg acggcgagcg
    15361 gctccagggc atggtcaccg accgcgacct cgccgtcggc gtcctcgcca agggcaagga
    15421 ccccaagacc tgcagcgccg ccgacctggt cgacggtcgt gaggtcgtca ccatcggcgc
    15481 cgacgacgac atcgacgagg ccatcgacac gatgaagaac cacgccgtgc gccgcctccc
    15541 ggtcatcgac ggcgacaagg tgatcggcct ggtcgcgcag gccgacatcg ccaagcacgg
    15601 cgacgacaag caggtcggcc gcctggtgga gacgatctcc gaggccccgg acaactccgg
    15661 caagggctga gtcgcctccc tccatctgga gatcccgtgg atcccgaccc acccgtggtc
    15721 gggatccacg gcgttttcag gtggtgcgga tgcggcgggc cacgcgggcc acgtcgtcgc
    15781 cgggccgcac gggccgcagc gcccagctcc agcgggccgg tccgcccgtg acctggtacg
    15841 gcgccagcgt gtccgggccg cacgcgccgg tgccgaggcc gcggttgacg aggtcgacgt
    15901 ggacctcgat ggtcgggctc atctgcagct cggcggtggt ggtcgcggcg tagaggtcgg
    15961 cgtcgtggtg gtggcgggcg ctgaacccga agggctcgag gcgatcggtc gacgacacca
    16021 ggacaccgga cccaccctcg gccgggtcgc cgtcggcgag cacgaaccac cgggtgtcgg
    16081 tgtggtgccc gtgctcctgg ggcagcacgt agggcacgta ctggtcggcc acccgtcccc
    16141 gccaccgacc cagctgctcg ctgctctccc ggtcggcgta ggactccagc ggaccccggc
    16201 cgtaccactc gagcaggtcg agcgaggccg gcacctcgaa gcgggcgccg atgcgcggga
    16261 ggtcgtcgag gctgcggggc agcgccagct cctcgtcgac gacgacggtg ccgtcgccct
    16321 gcacccggag ccgctggcgg tggtcgatcg ccttgtccag ccccggcagg tgcaggcgcc
    16381 ggcgcagcgt cacctgcacc gacccatcgg cctggcgccg ggccgtgacc ttgcgggcct
    16441 cctgctcgac ccggtcgagg ccgagcgcga gccagccggc cagcgcgccc gggacggtgg
    16501 tgctgtcgag gcggcggcca tcgttctcgg tcggggcccg ccacaggctg gcggtgaccg
    16561 gtccggccag cagcgagcga ccgtccagca cgacatcgac cagcgctgcg acgtccgggt
    16621 cccaccggac ggtcacgtcg ccggcaaccg ccacgtcgcc gtcgagctcc accggttcgg
    16681 tcgtcggccc ggcgctcgac cccgtggccg gatgctgctt ggtctcgggg aggtcgaact
    16741 gctgccaggc gacccggtgc ccgaccgggg cccacggctg ctcctcggtc acgacgcagc
    16801 ggaggccgag gtgggcctcg gcgccgggtg gcacgtccac cgtggggagg tcgatctcga
    16861 ccgtggcctc gaacccgccg ggcagctccg ggaggtcgaa cgtgccggac gcgaccacct
    16921 cgccgtcgac ccgcacgtcc cactcgccac cgagccagga cacgtcgcgc cagtcctgca
    16981 tgttgatcac gacgatgcgg ccgcgcttga ggtcgctggc ccggaaccgg accggggcgg
    17041 tcaggtaggc gtactcgagc agcgccggct tcgggctgcg gtcgggccag accaggccgt
    17101 tgcagcagaa cgtggcgtcg ttgggttcgt cgccgaagtg gccgccgtag gcccagaact
    17161 cggcgccggc atcgtcggtg gcccgcagtc cctggtccgc ccagtcccag atgaacccgc
    17221 cctgcagccc gtcggtggtc tcgatcaccg cccagtagtc agcgaggctg ccgttggagt
    17281 tgcccatcgc gtggctgaac tcgcacatga cgagcggccg ctgctcgtcg gtgtcggcgg
    17341 ttcgctcggc ccaggcccgg atggcgtcga tcgacgggta catggggcag acgaggtcgg
    17401 tggccggacc ggggtcgccc gggtcgtcgg tgccggcgaa ggcgcggtgg caggccccct
    17461 cgtagtggac gacgcggctg gggtcggtcc ggcggatcca cgccgcggcg gcgtcgaaga
    17521 cggccgcgtc gcccgactcg ttgccgaggg agaagccgaa gatcgacggg tggttccggt
    17581 cgcggatcac cgtccgcacg atccggtcga ggatcgccgg cgtgaaccgg ggatcgagcg
    17641 acaggctggc cagccgggcg tggctctcga cgttcgcctc gtccaccacc cacaggccga
    17701 gctcgtcgca ggcgtcgagg aaccgggggt cgttcgggta gtgggcggtg cgcaccgcgt
    17761 tcacgttgtg ctgcttcatc agcacgacgt catcccacat gccgacctcg gtgaccgcct
    17821 tcccccggtc ggggtcgtgg tcgtggcggt tcaccccgtg gatcagcacc gggtacccgt
    17881 tgagcagcag ctcgcggtcg accacctcca cccgccggaa gccgatgcgc accgacgtcg
    17941 actcgaccgc gttcccgtcg gggtcgacca gcgacagcac cagcgtgtgc aggacggggt
    18001 tctccgacga ccacggcgac acgtcgtcga agcgctcgcg caggcgggtg acgccgcggt
    18061 ggaggtaggc gctgatcgac tccgccagcg gatcgctgcg gtcgaacacc tccacatccg
    18121 cgctgagcgg ctggcgcagc agggcggcgc catcgtcgtc gagcaccgag gcctcgaggc
    18181 gccaccccgc gtcgagcggc ccgcccaccc ggacgtcgac ctggagcagg ccggtggaca
    18241 ggtcctcgtc gaggtcggcg gtgacggcca cgtcggcgag gtgcacgtcg gcggtggagt
    18301 acaggaacac ctcgcggtgc aggcccgcca tccaccagtg gtcctggtcc tcgagccagc
    18361 tcccgtcgga ccaccggacc acgaccagcg ccagcgtgtt gcccccggcc tgcaggaacg
    18421 gcgtgaggtc gaactcctgg ggcaggcggg agtccttgcc catgccgacc ggctcgccgt
    18481 tgcagaagac gtagaggcag ctctccgcac cgccgacgtg cagcaccgcg cgacggccgt
    18541 cccagccgtc ggggagggtg aaggtgcgcc ggtgcacgcc ggtcgggttg gcgtcgggga
    18601 cctcgggcgg ttcgaggtcg aagggcatcc ggacgttcgt gtagtgcggc ttgtcgggga
    18661 cctccgggtg catggtccag cagcccggca cctcgaacgg cagccagccg tcgtcgaccg
    18721 cgtcggcgcc gacgtcctcc ggcgtcaccg cctcggggcg gtcgcgcagc gcgaagtccc
    18781 aggtgccatc gagggggtgg aaccagggag acgcggtccg atcgccgccg gcggccaggt
    18841 cctcgtcggg gtacgggtag gcggtcgccc gcatgggcag gcggccgaca gccacctgct
    18901 cgggtcgggc ccacgagcgc gggccgaacg cgtcgagggg cgagcgaggg ggcacggggt
    18961 ccgtcgccat cgggtcgacc gtagtttgtg ctccgtgccg ccgctcccgc ccttgcgccc
    19021 ccacctgcgt ccgggcgtgg tggtgcgagc cgccctgctc ggcacggtcg tcgcccgctt
    19081 cgtccgggcc accgcggccg aggccccgct cgccgcaccg cagccaccga ccgaccagcc
    19141 cgatgccacc gtcgtcgtcc ccgcccgaga cgagcaggac cggatcggcc cgtgcgtgac
    19201 ctcattggtg gggcaagggg cgcacgtgat cgtcgtggac gacggctcca ccgaccgcac
    19261 ccgtgaggtc gccgcggcgg ccggtgccac cgtcgtcgac gccggcccgc tcccggacgg
    19321 ctgggccggc aaggcgcacg ccctgcaggt ggggctcggc gcagcgtcga cgccggtggt
    19381 ggtcttcgtc gacgccgaca cccgcgcccg ggagggcttc gtcgcagccg ccgtcgatgc
    19441 actcggcgac tcgacgctcg tcacggccgg ggcacgcgtc ctggtcgacg acgccggcga
    19501 gcgctggttg cacccatcga tgctcaccag cctcgtgtac cgcctcggac cacccggggt
    19561 ggtcccgtcc gatccggccc gcacgatggc gaacggccag tgcatggtcg tcgaccgcga
    19621 gcggctgctc gacgccggtg gcttcgagcc ggtgcggggc cacctgctcg aggacctcgc
    19681 cctcgcccgc tcgctcgccg ggctcgggca cgacgtgcgc ttcctcgacg gcacggcggt
    19741 gatcgacgtc gagggctacg gatccctcgg cgccacgtgg cgggggtggg gccgctccct
    19801 cgacctgcgg acggtgacgc cgcccgccgc gcagctgctc gacctggcgg tggtctggtc
    19861 gacgatggca ctgcccctcc cccgcctggt ggctggccgg ggcgacgccg tcgacgtcgc
    19921 agcgctgctc gtgcgcctgg ggaccctcgc cggcacccgc ggcgcctacg cccggcgtgg
    19981 cctgccgtac gtgctctcgc cgctggccga cctcgcggtc gcggctcgca tcaccgccgg
    20041 cacggtccgg ccgagccggc gctggcgcgg gcgcagctac tgacggtgtc ccagagacga
    20101 acctcccggt tctgggccgc tccacacgca tgagagacgc gtacggcggc ccagttcgtt
    20161 tgatctcgtc cgtccggtca cgggtcggat cgatctcggc ttgcctgggc gaagcgcagc
    20221 ccgatgatcg acagcagcgc gccggtcgcc gccacccgct ccttgctctc gaaggccagc
    20281 tgcacgatcg ggaagccgaa gaccccgacc cgcgcggccc acgcgaacga ccgggtcgcc
    20341 gcccacacca ggagcaggac cagcaccgtg accgccgcgg gcacgggggc gagcacggcc
    20401 atgccgccga cgaacgtgag gatcgagcga ccaccccgga agcccgcgaa cacggggtag
    20461 gcgtggccga ccatcgctgc gccgaccgcg gcgtgggcga tgccccaggc cgggtcgtcg
    20521 agcagcaggc cgagcgcgcc ggccgccgcc cccttggcca cgtcgccgac gaacaccggg
    20581 agcgagctgc gcggtccgag ctgctccttg acgttccagt agccggggtt gcggtcgccg
    20641 acgtcccggg gatcgaaccc ccgagcccgc ccgaccagca ccgccaccgg gatcgaaccg
    20701 aggaggtagc cggccacgac ggcggtgagc agggcggcga gcaccgcacc actctgatcc
    20761 acggcccgta gcgtcggggg cgcatggtcg tcgcctcctt ccacctcgtg cggcatgccg
    20821 gcgccctcgc accgacggcg cgcctcccgc tggaccggcg agcgcttcgc caggtgcccg
    20881 ggctgcgctt cggtcggctg ctcggcaccg gcgagggccg gacgatgacg acgtcggccg
    20941 acctgcgacg ctgggccgcc ttcgccgtgt gggacgacat cgccgatctc gaccggttcc
    21001 gccgcgacca cccgctgtca cgtcgctggg accgagccgc ggagcgcttc gacgtgcagc
    21061 tgcggccgat cagcgcacgg ggcacgtggg acggcgtcga tcccctcgcc ggcgaggacc
    21121 acgccggtgc cctggacggc ccgatcgccg tgctcacccg ggccaccgtc cgctggcggc
    21181 gcctccctgc gttcctgcgg gcggtccccc cggtcgacgc gcacctcggc tcggtggacg
    21241 ggctgctcca ggcggcgggg atcggcgagt ggccgatcgg ccgccaggcc acgttcagcc
    21301 tgtggcgcga ccccgaggcc atgcagcggt tctcgtaccg gtcggccgag cacgtcgagg
    21361 tcgtgcgccg gacgcgcgac gaggactggt acggcgagga gctgttcgcc cggttcaccc
    21421 cggtggcgtg gaccgggacg tggtccggcg aacggccgct cggccccgac acctgggcgt
    21481 gacggcctgc cgtaccgtgg ggccatgcgc cgtgcactgc tgctgggact gctcaccgtc
    21541 gccctgatcg ccgccgggtg cggcgacgac gacgacaccg agaccaccac cgacacgtcg
    21601 gctgccaccg agaccgagac cgacacggtg accgagaccg agaccgagac cgccgatccg
    21661 acgtcgacca ccgccgcccc gacgccgtcg gagctgcccg gcgagcgcat cgagatcttc
    21721 ccgtacgaag gcaacgagct cgtcgtggtc ggcgtcgagg ccgacgacgt cctcaacgtc
    21781 cgcgccgcgc cgggcgtgga gtacgagatc gtcgccgagc tcgacccgct ggccgacggc
    21841 ctcatgccca ccgggcacaa ccgccagctc gacgacggcc cgatctgggc cgagatcgag
    21901 gtcgacggcg agaccggctg ggccagcacc gccttcctcg cccaccccgg cgcggtccgg
    21961 gacacgacca gcgagctcta ccccgacgtc gccgaccgtc cccgcggcga caccatgctg
    22021 cagctcggcg agctcgtcgc cgaacgggtc gccggcgagg gtgagcccga gcccgacgtg
    22081 gtcgtggtcg acggtcccac ggtcggcgac ctcggcgaga tcacggtcga cgtcgtgggc
    22141 ttccccgacg actccgtgct cggcgagcgc ctccacatct tcgccgagcc cgatccgggc
    22201 ggcgagagct tcaccgtgcg cacggtggag tccaccacgc tgtgccggcg tggggtcagc
    22261 gacggcctgt gcgtgtgagc tgccgctcgt cggccgccgc ggcccgggcc cgcacggagg
    22321 caggcacgat cggcaccagc atcccgtagg gctcgcctcc gaagcggtgg tgcagcgcgt
    22381 gcgcctcggc cagccggtcg agcacggggt gacgtcctcg cacggggagc cgctggtgga
    22441 tgtagccgtc gtggaccagc gcgtaggcga cgccgtacag ggtgacgccc acggtgacgg
    22501 gcagcagcac gctgaagccg tcgacgttga agccgatcgc catcgccacc atcacggcgc
    22561 cggcgaagat ggccgggaac gcgtcgttgg cctcgaagaa gggctcgccg tcggcacgcg
    22621 ccggccagcg gcggtggtgc gagcggtgga ggcggcggcc gaggccgtgc atcacgtacc
    22681 ggtgcgccag gtaggtgacc ggctccatca ccacgaaggc cgccaccacg acggcgagcg
    22741 cggtcacgcc ggtccctctg gtcgccacgg tgccgggtcg tccggggtgt cctggcggac
    22801 ccagaggagc aggacgaggg tgaggagcga gaagccgaag aagtagtcct cgaccgggat
    22861 gtcccagggg aagcgccacg gggtcttctg gtccgggtcg tagcgcacga tcggcgccga
    22921 cagcttcgtg agccacccgt tgaccaggaa catgaagaac aggctgat
//
LOCUS       DATISU010000329         5808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_768097, whole genome shotgun sequence.
ACCESSION   DATISU010000329 DATISU010000000
VERSION     DATISU010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768097"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VLR27_13110"
     CDS             complement(<1..583)
                     /locus_tag="VLR27_13110"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HSP04441.1"
                     /translation="MELLLRVHTDRRSPLDVAVDIDADEPVARLAEALGEATAIEGVD
                     LRVSSARTGQVFDPDEPVGATGIVSGDELVLGWAQVRPATAAVPIRGVSVDVLAGPDS
                     GHSAVLDRGSYLLGRDQSADLTFGDVTVSHLHLRVDVEADWTVRIAPHEDPQNGVLVN
                     DEPIDEATVVGPDDVVALGSSRVSFREFIRAEHE"
     gene            complement(597..3596)
                     /locus_tag="VLR27_13115"
     CDS             complement(597..3596)
                     /locus_tag="VLR27_13115"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HSP04442.1"
                     /translation="MIRRILREALVPALLLAAATATFAPAFDVDALARAGVVSALVGG
                     AAGLLLRWRVPLLAGPLVVLAAIAAAAALAPPSPVWPGDIGTAVSTFLSVGLPADGLD
                     DLLVVVALVVTPAALLTVGASLRRRPILTLAGPVVAAVVGAVLIAPTAPPTWPAVALA
                     VVGCAVLGADARSDISAMPPLVGSRTEERRQRSWWRPAAQLGAALAAVALAAVLPLPG
                     SADLRDVVQPRTLRVEDPNPLSVAARWGQVENPVTVAEVTVDGPAPGRFRLSVLEDYT
                     PTGWRQAADFLVTGRSLAPDPRGIAPLPDQDAGGAAGTTVTVTPTEHLAPFRAVPTAG
                     QPLAVADPDGLRYAPDAGVVLAGRQRPVSYRTEVAPVPGDLAAGLVPTVVPDVPPELL
                     ACPDSDAVRAVATQLTAGIGPFEDRLDALEDWLLTRRIYDPAAPGGQTLAGVDTFVRT
                     TFGRGNLEVFVTTYALLARCAGVPVRVVVGAADVPEGSTEITQDAIGAWVEVPVDGQG
                     WVARDPLPTPEEQEAVAQLAEQPPPPPEEPETSEPSEPEQVEALDPPSDDAPIWPFVL
                     VALVVLAVLAVAVLLPRWVHRRRRRIADPTAAVLAAWTTVLDELADRRIPVSGAHTPQ
                     EAAGAVASHVPIPVRRAIELLAPLVDGARYRGTPATDEEASTAWALADLARVRLPRGR
                     ADHLSALVHPLRTVSRVRSVLGVERRSKAWEATLPPEVAAASEEAPTDVPGVTLDTRI
                     GAGSTGTVFKGTLVETGGDVAVKVFRFGPGDDGFDERRFDWEIHIAREVSGYPHLPEV
                     LGAGTTPGGVRPYLITTLYEDGTLLDRVRRGGPLTTAEAVAVGADVATALATLHQLGV
                     VHGDVKPENAFAGREGWVLGDLGSAWLRAGRGPARSLTPPYAAPEVWRGSSPTPASDL
                     YSLGLTVLFAATGDVPVAGNAPDLVQVRALFGDHSDLARLVDPDPRRRPRSAAELARR
                     LRPGARAAAFADGGLSLPTPTVTTARP"
     gene            complement(3593..4717)
                     /locus_tag="VLR27_13120"
     CDS             complement(3593..4717)
                     /locus_tag="VLR27_13120"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSP04443.1"
                     /translation="MQLTGTGRALVAAAVVLTVAGVALGQPGTVGLGVAGLLVTLVAA
                     LTVAEAPKVEVDRAVRPHEVDRGKPAEVSLAFRGTAPRARPFSVVETVGGERRSAAMP
                     ALRAGERASIAYELDTTRRGTIVAGPLQLRRTDPLGLVVAERRIAGTASVQVRPRRHP
                     IAMLPSGRWRDLEGPTREVSRGTATFHQLRDYVPGDDMRHIHWRATARTGDLIVREHV
                     DTTKPEVVVIVDNRERALGEDDFEEGVDVAASVLTAAERAGFPTRLLFADGREERDEG
                     LDQLAHLDRLTAVRRSPVASLQQLADALRARGRSLVFVTGELDPQDLRLLAKIAHGFA
                     PAILVSVQADRRAPFVAPPGVVGIPCASAGGFVAAWGQHR"
     gene            complement(4722..>5808)
                     /locus_tag="VLR27_13125"
     CDS             complement(4722..>5808)
                     /locus_tag="VLR27_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSP04444.1"
                     /translation="AVTDHEPTNGNPIPEPPSATPSFQRRLAAPAMPSPRAASAPASE
                     QDIVWFAETFSRLTANICRVIRGKNDEVNLALLGLVSEGHLLLEDAPGLGKTSLARAL
                     AESISGDWQRIQFTPDLLPSDITGVSVWDPGSRHFEFRPGPVFANVVVGDEINRASPK
                     TQSALLEVMEERQVTVDGHSRPVPTPFVVVATQNPVEMDGTYHLPEAQLDRFLLKMSL
                     GYPDLAAEVDIVLSRHGSAVVHLEPVVTLAEVQRMADISASVRLDPQLVQYAVSVVHA
                     SRTSADVRLGASPRGSIGLLRAAQSLAAAEGRTYAIADDVKRLAVPVLAHRLMLSPDA
                     QLRGVTAERVVTELLATTAVPGGAEVG"
BASE COUNT          762 a         1990 c         2412 g          644 t
ORIGIN      
        1 cctcgtgctc ggcccggatg aactcccgga agctgacccg gctcgacccg agcgccacca
       61 cgtcgtcggg gccgacgacc gtggcctcgt cgatgggctc gtcgttgacg aggacgccgt
      121 tctgggggtc ctcgtggggc gcgatgcgga cggtccagtc cgcttcgacg tccacccgca
      181 ggtgcaggtg ggacacggtc acgtcgccga aggtgaggtc ggccgactgg tcgcgcccca
      241 gcaggtagga gccgcggtcg agcactgcgg agtggccgga gtcggggccg gcgagcacgt
      301 cgacgctcac ccctcggatc ggcacggcgg ccgtggccgg tcggacctgg gcccagccca
      361 gcaccagctc gtcgccggac acgatgccgg tggcgcccac cggctcgtcg gggtcgaaca
      421 cctggccggt gcgggccgag gacacccgca ggtcgacccc ctcgatcgcg gtcgcctccc
      481 cgagcgcctc ggcgaggcgg gcgaccggtt cgtcggcgtc gatgtcgacc gccacgtcga
      541 ggggcgatcg gcggtcggtg tgcacgcgga ggagcagttc catcacaggt tccggatcag
      601 ggtcgggcgg tggtgaccgt gggggtgggc aggctcaggc cgccgtcggc gaacgccgcg
      661 gcgcgggccc cggggcggag gcgtcgggcg agctcggcgg ccgagcgggg gcggcggcgg
      721 ggatcgggat cgacgagtcg ggccaggtcc gagtggtcac cgaacaaggc tcgcacctgg
      781 acgaggtcgg gggcgttgcc cgcgaccggg acgtcgccgg tggcggcgaa cagcacggtg
      841 agaccgaggc tgtagaggtc ggaggcgggg gtggggctcg acccgcgcca cacctcgggc
      901 gcggcgtagg gcggggtgag actgcgggcc ggaccgcgac cggcgcggag ccacgccgag
      961 ccgaggtcgc cgagcaccca cccttcccga ccggcgaagg cgttctcggg cttgacgtcg
     1021 ccgtggacca cgccgagctg gtggagcgtg gcgagcgcgg tggcgacgtc ggcgccgacc
     1081 gcgacggctt cggcggtcgt gagcggcccg ccccggcgga cccggtcgag cagggtgccg
     1141 tcctcgtaca gggtggtgat caggtacgga cggacgccgc cgggggtggt gccggcgccg
     1201 agcacctcgg gcaggtgcgg gtagccggac acctcgcgtg cgatgtggat ctcccagtcg
     1261 aagcggcgct cgtcgaagcc gtcgtcgccg ggcccgaagc ggaacacctt caccgccacg
     1321 tccccgccgg tctcgacgag ggtgcccttg aacacggtgc cggtggagcc ggccccgatc
     1381 cgggtgtcga gcgtgacgcc ggggacgtcg gtgggcgcct cctcgctggc ggccgccacc
     1441 tcggggggca aggtggcctc ccacgccttc gagcggcgct cgacgccgag caccgaacgg
     1501 acccggctca cggtccgcag cgggtggacg agggcagaga ggtgatcggc gcgcccgcgg
     1561 ggcaggcgca cccgggccag gtcggcgagc gcccacgcgg tgctcgcctc ctcgtcggtg
     1621 gcgggcgtgc cgcggtagcg ggcaccgtcg accagcgggg cgagcagctc gatggcccgg
     1681 cgcaccggga tgggcacgtg ggacgcgacg gcgcccgccg cctcctgcgg ggtgtgggcg
     1741 ccggacaccg ggatccgccg gtcggccagc tcgtcgagga cggtggtcca ggcggcaagg
     1801 accgcggcgg tggggtcggc gatccggcgc cgccggcggt ggacccaccg ggggagcagc
     1861 acggcgacgg ccaggacggc gagcacgacg agtgcgacga gcacgaacgg ccagatcggc
     1921 gcgtcgtcgc tcggcgggtc gagcgcctcg acctgctcgg gctcggacgg ttccgacgtc
     1981 tccggctcct cgggtggggg tggcggctgc tcggccagct gggcgacggc ctcctgctcc
     2041 tcgggggtcg ggaggggatc gcgggccacc cagccctgcc cgtcgacggg gacctccacc
     2101 cacgcgccga tggcgtcctg ggtgatctcg gtggagcctt cggggacgtc ggcggcgccg
     2161 accaccaccc gcacggggac gccggcgcag cgggccagca gggcgtaggt ggtgacgaag
     2221 acctcgaggt tgccgcgccc gaaggtcgtg cggacgaagg tgtcgacgcc ggccagcgtc
     2281 tggccgcccg gtgccgcggg gtcgtagatg cgccgggtca ggagccagtc ctcgagcgcg
     2341 tcgagccggt cctcgaacgg cccgatgccg gcggtcagct gggtcgccac cgcccgcacc
     2401 gcgtcgctgt cgggacaggc gagcagctcg ggcgggacgt cggggaccac ggtggggacc
     2461 agtccggcgg cgaggtcgcc gggcacgggc gcgacctcgg tgcggtagga gaccggtcgc
     2521 tgccggccgg ccagcaccac gccggcgtcc ggggcgtacc gcaggccatc ggggtcggcc
     2581 acggccaacg gctgcccggc ggtgggcacg gcccggaacg gcgccaggtg ctcggtgggg
     2641 gtgacggtca ccgtggtgcc cgcggcaccg ccggcgtcct ggtccgggag cggggcgatg
     2701 ccgcgggggt ccggcgccag ggaccggccg gtcacgagga agtctgcagc ctggcgccac
     2761 ccggtgggcg tgtagtcctc gagcacggag aggcggaacc gccccggtgc gggaccgtcg
     2821 acggtgacct cggcgacggt gacggggttc tcgacctgac cccagcgggc ggcgaccgac
     2881 agcgggttgg ggtcctccac ccgcagggtg cggggctgga ccacgtcgcg caggtcggcc
     2941 gacccgggga ggggcagcac cgccgccagg gcgacggcgg cgagggcggc gccgagctgg
     3001 gcggccggtc gccaccacga gcgctgccgg cgctcctcgg tgcggctgcc caccagcggc
     3061 ggcatggccg agatgtccga gcgggcgtcg gcgccgagga ccgcgcagcc gaccacggcg
     3121 agggcgacgg ccggccacgt cgggggcgcg gtgggggcga tgagcacggc accgacgacg
     3181 gcggcgacga ccgggccggc cagggtcagg atcgggcggc ggcggagcga tgcaccaacg
     3241 gtcagcaggg cggccggggt gacgacgagg gcgacgacga caaggaggtc gtcgagcccg
     3301 tcggcgggca gacccaccga caagaacgtg gacaccgccg tgccgatgtc gccgggccac
     3361 accgggctgg gcggcgcgag cgccgcggca gcagcgatgg cggccagcac cacgagcgga
     3421 ccggcgagca gcggaacacg ccatcgcagc aggagcccag cggcgccgcc gaccagcgcc
     3481 gacaccacgc cggctcgggc gagggcatcg acgtcgaacg ccggggcgaa cgtggcggtc
     3541 gccgcggcca gcagcagtgc cggtaccagc gcctcgcgga ggatgcggcg gatcaccggt
     3601 gctgccccca cgcggcgacg aacccgccgg cggacgcgca cgggatgccg accacgccag
     3661 gaggggcgac gaagggcgca cgccggtcgg cctggaccga gacgaggatc gccggggcga
     3721 acccgtgcgc gatcttggcg aggaggcgga ggtcctgggg gtcgagctcg ccggtcacga
     3781 acaccaggct ccggccgcgt gcgcggaggg cgtcggcgag ctgctggagc gaggccacgg
     3841 gggagcggcg cacggcggtg agccggtcga ggtgggcgag ctggtcgagg ccctcgtcgc
     3901 gctcctcgcg gccgtcggcg aacagcaacc gcgtggggaa cccggctcgc tcggctgcgg
     3961 tgagcacgga cgcggcgacg tcgacgccct cttcgaagtc gtcctcgccg agggcgcgtt
     4021 cgcggttgtc gacgatcacc acgacctcgg gcttggtggt gtcgacgtgc tcgcgcacga
     4081 tcaggtcgcc ggtgcgggcg gtggcgcgcc agtggatgtg gcgcatgtcg tcgcccggga
     4141 cgtagtcgcg cagctggtgg aaggtggccg tgccccggga cacctcacgg gtcgggccct
     4201 cgaggtcgcg ccagcgcccc gacggcagca tggcgatcgg gtggcggcgg gggcgcacct
     4261 ggaccgatgc ggtgccggcg atgcggcgtt cggccaccac cagaccgagg ggatcggtgc
     4321 ggcgcagctg cagcggaccg gccacgatcg tgccccggcg ggtggtgtcg agctcgtagg
     4381 cgatggaggc tcgctcgccg gcccggagcg ccggcatcgc ggccgatcgc cgctcgccac
     4441 ccaccgtctc gaccacggag aacggccggg ctcggggtgc ggtgccccgg aacgccagcg
     4501 acacctcggc cggcttgccg cggtcgacct cgtggggccg cacggcgcgg tcgacctcga
     4561 ccttcggcgc ctcggccacg gtgagtgcgg ccaccagggt cacgagcagg ccggcgacgc
     4621 cgagaccgac ggtgccgggc tggccgagcg cgacgccggc cacggtgagg accaccgccg
     4681 ccgccacgag cgccctcccg gtgccggtga gctgcacggg ttcagccgac ctcggcgccg
     4741 ccgggcacgg cggtggtggc gagcagctcg gtgacgacgc gctcggcggt gacgccccgc
     4801 agctgggcgt cgggcgagag catcagccgg tgagcgagca cgggcacggc caggcgcttc
     4861 acgtcgtcgg cgatggcgta cgtgcggccc tcggcggcgg ccagcgactg cgcggcgcgc
     4921 agcagaccga tgctgccgcg ggggctggcg ccgagccgga cgtcggcgga ggtgcgcgac
     4981 gcgtgcacca ccgacaccgc gtactggacc agctggggat cgagccgcac cgacgccgag
     5041 atgtcggcca tgcgctgcac ctcggccagg gtgaccaccg gctcgaggtg gacgacggcg
     5101 ctgccgtggc gggacaggac gatgtcgacc tcggcggcga ggtccgggta gccgagcgac
     5161 atcttcagca ggaaccggtc gagctgggcc tcgggcaggt ggtaggtacc gtccatctcc
     5221 accgggttct gggtggcgac caccacgaac ggcgtcggca cgggccggct gtggccgtcg
     5281 acggtgacct ggcgctcctc catgacctcg agcagggcgg actgggtctt gggcgaggcc
     5341 cggttgatct cgtcgccgac cacgacgttg gcgaacaccg gaccgggccg gaactcgaag
     5401 tgccgggagc ccgggtccca caccgacacg ccggtgatgt cggaggggag caggtcgggc
     5461 gtgaactgga tgcgctgcca gtcgccggag atcgactcgg ccagcgcccg ggccaggctg
     5521 gtcttgccga ggccgggggc gtcctcgagc agcaggtgcc cctcggacac caggccgagg
     5581 agggcgaggt tgacctcgtc gttcttgccc cggatgaccc ggcagatgtt ggcggtcagc
     5641 cgggagaacg tctcggcgaa ccacacgatg tcctgctccg acgccggcgc cgaggcggcg
     5701 cgcggggacg gcatggccgg ggcggccagg cggcgctgga acgacggggt ggcgctgggc
     5761 ggctcgggga tggggttgcc gttggtgggc tcgtggtcgg tcacggcg
//
LOCUS       DATISU010000330         4100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_768107, whole genome shotgun sequence.
ACCESSION   DATISU010000330 DATISU010000000
VERSION     DATISU010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768107"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1299
                     /locus_tag="VLR27_13130"
     CDS             106..1299
                     /locus_tag="VLR27_13130"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSP04445.1"
                     /translation="MTFDPDLPKTLDERMAHHVETGETGGIAWLASCDGKVVAGSAGV
                     LTRGEPAPVEQDSLFRIASITKPLTAVAALLLVEECRLRLEDPVDALLPELADRRVLV
                     DPLGPIDGDTVSAEGPITVRDLLTMQCGLGMDFTAPWPQPLLDAMGALGLSPGAPEPQ
                     VPPEPDEWMRRMGTLPLLRQPGERWLYNTASDILGVLVGRAAGMPFDRFLQERVLDPL
                     GMVDTGFSTTATDRLGTSYAVDPETGERSVYDGPDGKWSTPPEFPSGAAGLVSTVDDL
                     AAFGTMLLADGFLPDGSRLLSRASVAAMTTDQLGVAAGLHPSVSADGSQGWGFGVGVQ
                     VRRTGVARNVGAYGWDGGLGSSWANDPAEDLVGVVLSTDMFGGSDALPHPLADFWTTV
                     YAALD"
     gene            complement(1307..1804)
                     /locus_tag="VLR27_13135"
     CDS             complement(1307..1804)
                     /locus_tag="VLR27_13135"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HSP04446.1"
                     /translation="MATSTERTGVGAARRDYDLLRKVTAAVGLVFLLVGIAGFIPGIT
                     TDYSDMAVGGPDSEAKLLGIFQVSIVHNIVHLAFGVLGLVAAKAWGSARLFLVGGGII
                     YLALWLYGLVIDKDSDANFVPLNTADDWLHFGLGLGMLALGALLWNGRSARAGAARNT
                     ASRTR"
     gene            complement(1903..3264)
                     /locus_tag="VLR27_13140"
     CDS             complement(1903..3264)
                     /locus_tag="VLR27_13140"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HSP04447.1"
                     /translation="MTYRLDPDRPLVDEVRRAALECLDDAIGRLEEPGADLEDDVHET
                     RKRGKETRGSIRLVRPALGDAYASVNAEVRDGARELSSLRDAHALLGTVDDLASSPDG
                     NGTDELEALAGHLRVAASAATHRVDGDDPRIAVARARFETARAMIATWAGTIDVEALV
                     GGVAKTAARGRKAWKASRKDASDEQVHEWRKRVKYLWYQARLLEAADPDGIGPLVAQL
                     DELSDLLGDEHDLTVLVEHVERAADTDAMADVDVDRVIEVARARQATLRAAALELGEE
                     IYGDGVEALVDRLVGPWSGAGGMEGDGMERERTFLVADMPELPDDGTRIRQGYLAIDG
                     DVQVRVREREGDGRTLTVKGGRGAVRAEIELVIGVDRFEALWPLTAGRRIDKTRYVVP
                     VDGADAELDVFAGDLAGLVLVEVELDSDEAMAAFEPPAWFGPEVTDDDRYSNATLAVD
                     GLL"
     gene            complement(3306..>4100)
                     /locus_tag="VLR27_13145"
     CDS             complement(3306..>4100)
                     /locus_tag="VLR27_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSP04448.1"
                     /translation="VSCITSCGACRFCREGRYGQCLGGGGWILGHLIDGTQAEYVRVP
                     FADTSTYKAPAGVPDEELLMLADIIPTGYEVGVLNGKVTPGDVVAVVGAGPIGLATIM
                     GAKLFSPAHIVAIDKVDARLEAAKMFGADVVVNNDREDPLEVVRSLTEGLGADVAVEA
                     VGVPATFELSAELIRPGGHVANVGVHGEPATLHLEELWIKDVTITTGLVDTYSTPTLL
                     KLVTSRQVDARRFVTHHFTMDDFMEAYDVFTRAGETGALKVVVTRD"
BASE COUNT          542 a         1524 c         1450 g          584 t
ORIGIN      
        1 cgtccgaccg tacgaagggc tcccgggcgg cgacagggac tgcggaccct ttccggcgca
       61 gacctcgtgg cgatgatctg gcggcggtgg caacgtcgga gggacatgac cttcgatccc
      121 gacctcccga agacgctcga cgagcgcatg gcccaccacg tcgagaccgg cgagaccggc
      181 ggcatcgcct ggctcgccag ctgcgacggc aaggtggtcg ctggttcggc cggggtgctc
      241 acccgaggcg aaccggcgcc cgtcgagcag gacagcctgt tccggatcgc gtcgatcacg
      301 aagccgctca ccgcggtcgc cgcgctgctg ctcgtggagg agtgccggct gcgcctggag
      361 gacccggtcg acgccctcct gcccgagctg gccgaccgcc gcgtcctggt cgacccgctc
      421 ggcccgatcg acggcgacac cgtctcggcc gaaggaccga tcaccgtgcg cgacctgctc
      481 accatgcagt gcgggctcgg catggacttc accgcgccgt ggccccagcc gctgctcgat
      541 gccatgggcg cgctcggcct ctcccccggc gcgccggagc cccaggtccc gccggagccc
      601 gacgagtgga tgcgccgaat gggcacgctg ccgctgctgc gccaaccggg cgagcggtgg
      661 ctgtacaaca cggcgtcgga catcctcggc gtgctcgtcg ggcgggccgc cggcatgccc
      721 ttcgaccggt tcctgcagga gcgggtgctc gacccgctcg gcatggtcga caccgggttc
      781 tcgaccaccg ccaccgaccg cctcggcacg tcctacgccg tcgacccgga gacgggcgag
      841 cggtccgtgt acgacggacc cgacggcaag tggtcgacgc cacccgagtt cccgtccggt
      901 gcggccgggc tcgtgtcgac ggtcgacgac ctcgccgcct tcggcacgat gctgctggcc
      961 gacggcttcc tgcccgacgg gtcccgtctg ctgagtcggg catcggtcgc ggccatgacc
     1021 accgaccagc tcggcgtcgc cgccggcctc caccccagcg tctcggccga cgggtcccag
     1081 gggtggggct tcggggtcgg cgtgcaggtc cgccgcaccg gcgtggcgcg caacgtcggc
     1141 gcctacgggt gggacggcgg cctcggcagc tcgtgggcca acgacccggc cgaggacctg
     1201 gtcggcgtgg tgctctccac cgacatgttc ggcggttcgg acgcgctgcc ccacccgctc
     1261 gccgacttct ggaccaccgt ctacgccgcg ctggactgac gggacctcag cgggtgcggg
     1321 aggcggtgtt ccgggcggcg ccggctcggg cggaacgtcc gttccacagc aacgcgccga
     1381 gggcgagcat gccgagcccc agcccgaagt gcagccagtc gtcggcggtg ttgagcggga
     1441 cgaagttggc gtcgctgtcc ttgtcgatca ccaggccgta cagccagagc gccaggtaga
     1501 tgatgccgcc gccgacgagg aacagccggg ccgagcccca cgccttcgcg gccaccagac
     1561 ccagcacgcc gaaggcgagg tgcacgatgt tgtgcacgat cgagacctgg aagatgccga
     1621 gcagcttggc ctcggagtcc gggccgccca cggccatgtc ggagtagtcg gtggtgatcc
     1681 ccgggatgaa cccggcgatg ccgaccagca ggaagacgag gccgacggca gcggtcacct
     1741 tcctcaggag gtcgtagtcg cggcgtgccg caccgactcc ggtgcgctcg gtcgatgtcg
     1801 ccatgtctgt gctcctcgtg tctcggtggg aagccagggt ccagggcgtc gcggtccgtt
     1861 ccgcgcgtcg gtacccgggc gatccggcgc cgagaactcg gcctacagca gcccgtccac
     1921 cgccagggtc gcgttcgagt agcggtcgtc gtcggtgacc tcgggcccga accacgccgg
     1981 cggctcgaag gcggccatgg cctcgtcgga gtcgagctcg acctcgacca gcaccagccc
     2041 ggcgaggtcg cccgcgaaca cgtcgagctc ggcgtcggcc ccgtcgaccg ggaccacgta
     2101 ccgggtcttg tcgatccgcc gaccggcggt cagcggccac agcgcctcga agcggtcgac
     2161 gccgatcacc agctcgatct cggctcgaac cgcgccccgc ccgcccttca cggtgagcgt
     2221 gcggccgtcg ccctcccgct cccggacccg cacctgcacg tcgccgtcga tggccaggta
     2281 gccctggcgg atgcgggtgc cgtcgtcggg cagctcgggc atgtcggcga ccaggaacgt
     2341 ccgctcccgc tccatgccgt ccccctccat gccgccggcg ccggaccacg ggccgacgag
     2401 tcggtcgacg agggcctcca cgccgtcgcc gtagatctcc tcgccgagct cgagcgctgc
     2461 cgcccgcagc gtcgcctgcc gggcgcgggc cacctcgatg acgcggtcga cgtcgacgtc
     2521 cgccatcgcg tcggtgtcgg cggcccgctc cacgtgctcg accagcacgg tcaggtcgtg
     2581 ctcgtcgccc agcagatcgg acagctcgtc cagctgcgcc acgagcgggc cgatgccgtc
     2641 ggggtcggcg gcttcgagca accgggcctg gtaccagagg tacttcaccc gcttgcgcca
     2701 ctcgtgcacc tgctcgtcgc tggcgtcctt gcggctcgcc ttccatgcct tccgcccccg
     2761 ggccgcggtc ttggcgacgc cgccgaccaa cgcctccacg tcgatcgtgc cggcccaggt
     2821 ggcgatcatc gcccgggccg tctcgaaccg ggcccgggcc acggcgatcc gggggtcgtc
     2881 gccgtcgacg cggtgggtgg cggccgaggc cgcgacccgg aggtggccgg cgagagcctc
     2941 gagctcgtcg gtgccgttcc cgtcggggga ggaggccagg tcgtcgaccg tgccgaggag
     3001 cgcatgggca tcccggaggc tggacagctc ccgggcgccg tcacgcacct cggcgttgac
     3061 ggaggcgtac gcatcgccga gcgccgggcg gaccaggcgg atcgagcccc gcgtctcctt
     3121 gccgcgcttg cgggtctcgt ggacgtcgtc ctcgaggtcg gcgccgggct cctcgaggcg
     3181 gccgatcgca tcgtccaggc actcgagggc ggcgcggcgg acctcgtcga cgagcgggcg
     3241 gtcggggtcg agccggtagg tcacctccgg tcccttcccg acggccgccc gccggttgac
     3301 gtcgctcagt cccgggtcac cacgaccttg agcgcaccgg tctccccggc gcgggtgaac
     3361 acgtcgtagg cctccatgaa gtcgtccatc gtgaagtggt gcgtcacgaa ccggcgggcg
     3421 tcgacctgac ggctcgtcac cagcttcagc aaggtcggcg tggagtacgt gtcgaccagg
     3481 ccggtggtga tcgtgacgtc cttgatccac agctcctcga ggtggagcgt ggccggctcg
     3541 ccgtggaccc cgacgttggc gacgtgtcca cccgggcgga tgagctcggc cgacagctcg
     3601 aaggtggcgg gcacgcccac cgcctccacc gccacatccg cgccgaggcc ctcggtcagg
     3661 ctgcgcacga cctccagcgg gtcctcccgg tcgttgttca ccacgacgtc ggcgccgaac
     3721 atcttcgccg cctccaaccg ggcgtcgacc ttgtcgatcg cgacgatgtg cgccgggctg
     3781 aagagcttgg cgcccatgat cgtggcgagg ccgatgggtc cggcacccac caccgccacc
     3841 acgtcaccgg gggtgacctt gccgttgagc acgcccacct cgtacccggt cgggatgatg
     3901 tcggcgagca tcagcagctc ctcgtccggg accccggcag gtgccttgta cgtggacgtg
     3961 tccgcgaacg ggacccgtac gtactcggcc tgggtgccgt cgatgaggtg gccgaggatc
     4021 cagccgcccc cgccgaggca ctgcccgtag cgaccctcgc ggcagaagcg gcacgcgccg
     4081 cacgaggtga tgcaggacac
//
LOCUS       DATISU010000331         1823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_768139, whole genome shotgun sequence.
ACCESSION   DATISU010000331 DATISU010000000
VERSION     DATISU010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768139"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..1107)
                     /gene="map"
                     /locus_tag="VLR27_13150"
     CDS             complement(346..1107)
                     /gene="map"
                     /locus_tag="VLR27_13150"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HSP04449.1"
                     /translation="MGRRGRGARRSEAEIATMRRAGRVVAEMHDVCREAIRPGVTTGQ
                     LDALARDVLERRGATSNFLGYGGFPAVLCASVNDEIVHGIPGSRVLEEGDVVSIDCGA
                     VVDGWHGDAAFTTCVGQGSAADLELIEVGRRSLEAGIDQVVAGRTLGDLGHAVESVVR
                     AGGFSVLEDHCGHGIGRAMHEAPDVLNTGTPGKGLPIEVGMVLAIEPMVCAGTSDYRV
                     LADGWTVVTADGARSVHWEHTVAVTDHGPEVLTRH"
     gene            complement(1112..1771)
                     /locus_tag="VLR27_13155"
     CDS             complement(1112..1771)
                     /locus_tag="VLR27_13155"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HSP04450.1"
                     /translation="MIPGARLVMLGRQGAGKGTQCTRLSRHFVVPHISTGDMLRAAVK
                     EGTEFGRKAQEYMSAGELLPDDIIIGVVEERLLKDDTRNRGYILDGFPRTESQAESLA
                     EITADLPLDLVIDLAVPTDVVLERLAARRVCVDCGANYAVHAPPKHGWTCDHCGGDVI
                     QRDDDTEEAIKRRLDIYEAETAPLIQWYEARDLLMVVDGDGDPDEVTARLIRTIDTAR
                     R"
BASE COUNT          268 a          641 c          651 g          263 t
ORIGIN      
        1 cggatcacct ggcacttctc gcacatgggc ttgacactcg gtcggacctt catcggttgc
       61 tctctcgaga gtggttgctc agtggcaccg ggagccggcc acgactgagc tcgtacgggg
      121 gtgagctcca cctgcacccg atccccagga cgagacatgg gtgtgagcac aggccactgc
      181 aggggcaggc gcgcctgtcc cagagccgaa gggagatcct atgacagcgc gcacacgacc
      241 gccaacccca cgagcgacga ggggccggat cggtggcgcc gtcgagcccg tcagggcccg
      301 cgaaccccca catcatgacc gatccggaca cgcgatgggg ggatttcagt ggcgggtgag
      361 gacctcgggt ccgtggtcgg tcacggccac cgtgtgctcc cagtgcaccg agcgggcgcc
      421 gtcggcggtg accacggtcc agccgtcggc caggacccgg tagtccgagg tcccggcgca
      481 caccatcggc tcgatggcca gcaccatgcc gacctcgatg gggaggccct tgccgggcgt
      541 gccggtgttg agcacgtcgg gcgcctcgtg catggcccgc ccgatgccgt ggccgcagtg
      601 gtcctccagg acggagaacc ccccggcgcg cacgaccgac tcgacggcgt ggccgaggtc
      661 gccgagggtg cgaccggcca ccacctggtc gatcccggcc tccagcgaac ggcgcccgac
      721 ctcgatcagc tccaggtccg ctgcgctccc ctgcccgacg cacgtcgtga aggcggcgtc
      781 gccgtgccag ccgtcgacca ccgcaccgca gtcgatggag acgacgtcgc cctcctcgag
      841 cacccgggag ccggggatgc cgtggacgat ctcgtcgttc acgctggcgc agagcacggc
      901 ggggaagccg ccgtagccga ggaagttcga cgtggcgccc cgacgctcga gcacgtctcg
      961 ggccagcgca tcgagctgcc cggtggtgac gcccgggcgg atggcctccc ggcagacgtc
     1021 gtgcatctcg gccacgaccc gtccggcgcg tcgcatggtg gcgatctcgg cctcggaccg
     1081 gcgggcgcct cgaccacggc gacccatccc gctaccgccg agcggtgtcg atggtgcgga
     1141 tcaagcgggc cgtgacctcg tcggggtcac cgtcgccgtc gacgaccatg agcaggtcgc
     1201 gggcctcgta ccactggatc agcggggcgg tctcggcctc gtagatgtcg aggcggcgct
     1261 tgatggcctc ctcggtgtcg tcgtcgcgct ggatgacgtc gccaccgcag tggtcgcagg
     1321 tccagccgtg cttggggggc gcgtgcacgg cgtagttggc accgcagtcg acgcagaccc
     1381 gccgggcggc caggcgctcg agcaccacgt cggtgggcac ggcgaggtcg atgaccagat
     1441 cgaggggaag atctgcggtg atctcggcca gcgactcggc ctgggactcg gtgcggggga
     1501 agccgtcgag gatgtagccc cggttgcggg tgtcgtcctt gagcaggcgc tcctcgacga
     1561 ccccgatgat gatgtcgtcg ggcagcagct cgccggcgga catgtactcc tgggccttgc
     1621 gcccgaactc ggtgccctcc ttgaccgcgg cacggagcat gtcgccggtc gagatgtggg
     1681 gcacgacgaa gtggcgggag agccgcgtgc actgggtgcc cttgccggct ccctgccggc
     1741 cgagcatcac gagtcgggca ccggggatca tgagatcacg caccctaccg gccccgcttt
     1801 cgcccatccg aaatccccca ccc
//
LOCUS       DATISU010000332         2271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_70398, whole genome shotgun sequence.
ACCESSION   DATISU010000332 DATISU010000000
VERSION     DATISU010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70398"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VLR27_13160"
     CDS             complement(<1..189)
                     /locus_tag="VLR27_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04451.1"
                     /translation="MSDPLVEPIEGGEHQSFAGIDIDLFAVGDARVKRLVYGVGGKWS
                     EHVKPHVGTDWCEHAHVGF"
     gene            268..1986
                     /locus_tag="VLR27_13165"
     CDS             268..1986
                     /locus_tag="VLR27_13165"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HSP04452.1"
                     /translation="MPATSAPRSAGERLLPGLAVLRGYRRPWLRTDVFGGLTVGAMLI
                     PQSMAYAELAGMPPVTGFYAVIGALVIYALVGTSRHLGVGPEPGTAILAATGVGAIAA
                     GDPTRYLALMAALALVVAAICIAAAVVRLGFVSSLLSKPVLVGYITGVGLTLLSSQIA
                     GFTGVDIEGDSFFPRFTELLGELGDIHWSTLAVGAAALGVIVGLRRWAPQLPGALIGV
                     VGAGAAVALLGLDDDGIALIGAIPAGLPTPAVPDVSWSDLAALLPVAAGVALVGFTDN
                     LLTARSIAARRRDPEAAPIDANQELLALGLTNLSAGFSQGFPVSSSASRTAVPASLGS
                     TTQLVSLIAAASVVVSLTAGRSLLEHIPRPALAAVIIAAALAIIDAPGFRDLWRVSRT
                     DAALAVATTLGVIVFDVLVGVVVAVVLSVLAALSHMARPHDAVLGDLAGLDGWVDLDA
                     YPDATTEPGLLVYRFDAPLFFMNAGRFRARVEQVLEDNVGREEWLVLDFEGVGSLDTT
                     AVDALSELLEDLDEAEIAVVAVARAIQPVLDVLERSRLLAPAGPLVSFPTINAAVRAY
                     RDRAGS"
     gene            complement(1983..>2271)
                     /locus_tag="VLR27_13170"
     CDS             complement(1983..>2271)
                     /locus_tag="VLR27_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HSP04453.1"
                     /translation="KQTFIAGCGHAGDGNVHLSVFQPDPDVRYEMMLELFRLGMDHGG
                     AISGEHGLGRTKAKYWLELEDPTKVALMRRIKSAFDPNGILNPGALFGDTA"
BASE COUNT          252 a          841 c          817 g          361 t
ORIGIN      
        1 gaagccgacg tgcgcgtgct cgcaccagtc ggtgccgacg tgcggcttca cgtgctcgga
       61 ccacttgccg cccaccccgt agacgagtcg cttcacgcgg gcatcgccga ctgcgaacag
      121 gtcgatgtcg atgccggcga acgactggtg ctcgccgccc tcgatcggtt ccaccagggg
      181 atcggacatg gtgcggacgg tacccggccc ggcgccgcgc tacaggggaa ctgccagttc
      241 gagaggaccg ccgtacggtg acgctcgatg ccggcgacgt ccgccccccg aagcgcaggt
      301 gagcggctgc tcccgggtct cgcggtgctg cgcgggtacc ggcggccctg gctgcgcacg
      361 gacgtcttcg gcgggctcac cgtcggcgcc atgctcatcc cccagtcgat ggcctacgcc
      421 gagctggcgg gcatgccgcc ggtcaccggg ttctacgccg tcatcggcgc gctggtcatc
      481 tacgcgctgg tcgggaccag ccgccacctc ggggtcgggc ccgagcccgg caccgccatc
      541 ctcgccgcca ccggcgtcgg ggcgatcgcc gccggtgacc cgacccggta cctggcgctg
      601 atggccgcgc tcgccctggt cgtggccgcc atctgcatcg cggcggcggt ggtgcgcctc
      661 gggttcgtgt cgtcgctgct ctccaagccg gtgctcgtgg gctacatcac cggggtcggg
      721 ctcaccctgc tcagcagcca gatcgccggg ttcacgggcg tggacatcga gggcgactcc
      781 ttcttccccc ggttcaccga gctgctgggt gagctgggcg acatccactg gtccaccctc
      841 gcggtgggtg ccgccgccct cggcgtcatc gtcgggctcc ggcggtgggc gcctcagctc
      901 ccgggtgcac tcatcggggt ggtcggtgcc ggtgcagcgg tggcgctgct cggcctcgac
      961 gacgacggca tcgcgctcat cggcgcgatc ccggccggcc tcccgacgcc ggccgtgccc
     1021 gacgtgtcct ggtccgacct ggctgcgctg ctccccgtgg ccgccggcgt cgccctcgtg
     1081 ggcttcaccg acaacctgct caccgcccgc tcgatcgccg cccgacggcg ggaccccgag
     1141 gccgcgccca tcgacgccaa ccaggagctg ctcgccctcg ggctcaccaa cctgagcgcc
     1201 gggttctcgc agggcttccc ggtgtcctcc agcgccagcc gcaccgcggt cccggcatcg
     1261 ctcgggtcga ccacgcagct cgtgtccctg atcgcggcgg cgtcggtcgt ggtctcgctg
     1321 acggcgggac gatccctgct cgagcacatc ccgcggccgg cgctggccgc ggtgatcatc
     1381 gccgccgcgc tcgccatcat cgatgcgccc gggttccggg acctgtggcg ggtgagccgc
     1441 accgacgccg cgctcgcggt ggcgaccacc ctcggcgtga tcgtgttcga cgtgctcgtg
     1501 ggggtggtgg tggccgtcgt gctgtcggtg ctcgccgcgc tgtcccacat ggcccggccc
     1561 cacgacgccg tgctgggcga cctggccggg ctcgacgggt gggtcgacct cgacgcctac
     1621 cccgacgcca ccactgagcc cggcctgctc gtctaccgct tcgacgcccc gctgttcttc
     1681 atgaacgcgg gtcggttccg ggcccgggtc gagcaggtgc tcgaggacaa cgtcggccgg
     1741 gaggagtggc tggtgctgga cttcgagggc gtgggcagcc tcgacaccac cgcggtcgac
     1801 gcgctgagcg agctgctcga ggacctggac gaggcggaga tcgcggtcgt ggcggtggcc
     1861 cgggcgatcc agcccgtgct cgacgtgctc gagcgctccc gcttgctcgc gccggccggc
     1921 ccgctggtgt cgttcccgac gatcaacgcc gcggtgcgcg cctaccgcga ccgggcgggg
     1981 tcctaggcgg tgtcgccgaa cagggcgccg gggttcagga tcccgttggg gtcgaaggcc
     2041 gacttgatgc ggcgcatgag cgcgaccttg gtcgggtcct ccagctcgag ccagtacttg
     2101 gccttcgtgc ggccgaggcc gtgctcgccg gagatcgccc cgccgtggtc catgccgagg
     2161 cggaacagct cgagcatcat ctcgtagcgc acgtcggggt cgggctggaa caccgacagg
     2221 tgcacgttgc cgtcgccggc gtggccgcag ccggcgatga aggtctgctt g
//
LOCUS       DATISU010000333         1890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_629156, whole genome shotgun sequence.
ACCESSION   DATISU010000333 DATISU010000000
VERSION     DATISU010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629156"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="VLR27_13175"
     CDS             <1..1222
                     /locus_tag="VLR27_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04454.1"
                     /translation="GLVRRALAAALLGISLWIGSLAWTGFLLTRTVLDPDRSERVAEA
                     LYEDDAVRARLAANIADGVQASLPEGFPVDAATVDAGAAQALESPAVEAVFVDAFVRT
                     HQAMLGEGEVPRSVDPGAFGSAGRDALVAARPELDAILPAAPEVVVSLPTDRVPNLGP
                     VRRGLLTAVPWMAAASAVGAVLALLITSNRPAVIRRAGIWAVTLSAIVLAIAYGIPAL
                     ARTVVPDQSEVIAALVGAMVAETRVPAITLGALGVAGIVLSLFWRAAPALLSEPAPPQ
                     RRRTRPPRPRREPRLVGGPPPRRDISRPAPRPTPAAAPRQPDPSPTTVQPGPGRSHPE
                     LDADPTRVDASGSRPDPREGGNADLTTQQDVPSPPPGPTRWVAGVGWVVESAEHIPPE
                     ARWVPGVGYVVEEG"
     gene            complement(1201..>1890)
                     /locus_tag="VLR27_13180"
     CDS             complement(1201..>1890)
                     /locus_tag="VLR27_13180"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP04455.1"
                     /translation="LVERGWVCVSVTYRLSPRATWPDHIVDVKRALAWVKENIADHGG
                     DPSWIALTGGSAGGHLCALAALTPNDPQFQPGFEDADTTVQAAVPFYGVYDWLNRDGT
                     GRDDLTQLLVDRVVKQPFADARDLYEQASPMTHVGEHAVPMMLLHGTNDSLVPVAQAR
                     SMVDMLRAESKQPVVYVEYPGAQHAFDVFSGNRTDAAVAGIERFLNVARGDAGQHVHE
                     SAAEAQPSSTT"
BASE COUNT          213 a          723 c          694 g          260 t
ORIGIN      
        1 tgggctcgtg cgccgcgccc tcgccgccgc cctcctcggc atcagcctgt ggatcgggtc
       61 cctcgcctgg accgggttcc tgctgacgcg caccgtcctc gacccggacc ggtccgagcg
      121 cgtcgccgag gcgctctacg aggacgacgc ggtccgggcc cgcctcgccg ccaacatcgc
      181 cgacggcgtc caggcgtcgc tgcccgaggg gttcccggtc gacgccgcca ccgtcgatgc
      241 cggcgccgcc caggcgctgg agtcaccggc ggtggaggcg gtgttcgtcg atgccttcgt
      301 ccgcacgcac caggcgatgc tcggcgaggg cgaggtgccc cgctcggtcg acccgggcgc
      361 gttcggcagt gccggccgcg atgcgctcgt cgccgcccga ccggagctgg acgccatcct
      421 cccggcggcc ccggaggtgg tggtctccct gccgacggac cgggtgccga acctcggtcc
      481 ggtccgccgg gggctcctca ccgccgtgcc gtggatggcc gccgcctcgg ccgtcggggc
      541 ggtgctcgcc cttctgatca cctcgaaccg gccggccgtg atccggcgcg ccggcatctg
      601 ggcggtgacg ctctccgcga tcgtgctcgc gatcgcctac ggcatcccgg ccctcgcccg
      661 gaccgtcgtg cccgaccagt ccgaggtcat cgccgcgctg gtcggcgcga tggtggccga
      721 gacccgggtg ccggcgatca cgctcggcgc cctgggcgtc gccggcatcg tgctctccct
      781 gttctggcgg gccgccccgg ccctgctgtc cgagccggcg cccccgcaac gacgccgcac
      841 ccgaccgccg cgccctcgtc gcgagccgcg cctggtcggc ggcccgccgc cacgacggga
      901 catctcccga cccgcacccc ggcccacccc cgccgcagcg ccccgtcagc cggacccctc
      961 gcccaccacg gtccagcccg gccccggccg atcccacccg gagctcgatg cggatccgac
     1021 gcgggtcgat gcatcgggat cgcgtccgga tccgagggag ggaggcaacg ccgatctgac
     1081 gacgcagcag gacgtgccgt cgccgccccc aggcccgacc cgctgggtcg cgggcgtcgg
     1141 ctgggtcgtc gagtcggccg agcacatccc gccggaggct cggtgggtgc ccggtgtcgg
     1201 ctacgtggtc gaggagggct gagcctcggc ggccgactcg tgcacgtgct ggccggcatc
     1261 gccgcgcgcg acgttgagga accgctcgat gcccgcgacc gctgcgtcgg tgcgattccc
     1321 ggagaacacg tcgaaggcgt gctgggcgcc ggggtactcg acgtagacga cgggctgctt
     1381 cgactcggcg cgcagcatgt cgaccatgga ccgcgcctgg gcgaccggca cgaggctgtc
     1441 gttggtcccg tgcagcagca tcatcggcac ggcgtgctcg ccgacgtggg tcatcggcga
     1501 cgcctgctcg tagaggtccc gggcgtcggc gaagggctgc ttcaccaccc ggtcgaccag
     1561 cagctgggtg aggtcgtcgc ggccggtgcc gtcgcggttc agccagtcgt acaccccgta
     1621 gaacgggacg gcggcctgga ccgtggtgtc ggcgtcctcg aagccgggct ggaactgcgg
     1681 gtcgttcggg gtcagggcgg cgagggcgca caggtggcct ccggcggagc cgccggtgag
     1741 ggcgatccag ctcgggtcgc cgccgtgatc agcgatgttc tccttcaccc aggccaaggc
     1801 ccgcttgacg tcgacgatgt gatcgggcca ggtcgcgcgc gggctcaggc ggtaggtgac
     1861 cgagacgcag acccagccgc gctcgacgag
//
LOCUS       DATISU010000334         2581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_443735, whole genome shotgun sequence.
ACCESSION   DATISU010000334 DATISU010000000
VERSION     DATISU010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443735"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="VLR27_13185"
     CDS             complement(<1..1584)
                     /locus_tag="VLR27_13185"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HSP04456.1"
                     /translation="MYELARELGLENKETVDLCNSLGIGVKSHSSSIVEPQADRVRRK
                     AEREGLVREPKPDTGDGDGKPAAEAEAPATKKAAPAKKVVAKKAAPPKPKPAPEADQP
                     AAAEPSAPAAPTPPPASAPAAAQAGRADAPMPRPPAVRPTKGVVKSSGSEHPPPRPKE
                     DEPAAAAPAPPQPPAAAPPAPPAAPKPPSEAPKPPAAAPEPPAEAPAASAPKPPSEAP
                     KPPSAAPTPPPAAPPAPPEAPESPAASDAPRPPAAANDPGAKPGSKTPPKSASGKPIP
                     PPPGPMISRGGKPIPPPPGAGGRGPAPAPSGPGNRPARRGAAGQVRYTPPPANTGGPR
                     PGGGPGGGPGGGRPGPGGGRRPPKRRGGRRRRNRDELQPIEAPSFTPHDAPIPDHEVV
                     IERASTPQDIGPKLNRTASDVVRFLLEQGEMVTATQSLSDDMIELFAAELGATIRLVN
                     PGEEAEVELQKRLGVDELLGEDDEDDDSPSRPPIVTIMGHVDHGKTKLLDYIREANVV
                     AGEAGGITQHIGAYQAVKND"
     gene            complement(1939..>2581)
                     /locus_tag="VLR27_13190"
     CDS             complement(1939..>2581)
                     /locus_tag="VLR27_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04457.1"
                     /translation="AKALQPARVKEVRIDENTGTAMVIVPDHQLSLAIGKEGQNARLA
                     GRLTGWAIDIKSETQLAEAEAAYAAEDWAEGEWVVDEETGEQVWQPAEGGPAVSAAEY
                     MEGEEDEVEEPAVAEAAPDSDDTQVTEDADVAPADDEDVVEPLDPAADLAAAADDAVQ
                     AEVGDDPVERVQPDVPAGDVPSDAAEEQVLTTEPVVEGPELGGAADDPASEPT"
BASE COUNT          274 a          871 c         1032 g          404 t
ORIGIN      
        1 atcgttcttc acggcctggt aggcgccgat gtgctgggtg atgccaccgg cctcgccggc
       61 gaccacgttg gcctcgcgga tgtagtcgag gagcttggtc ttgccgtggt cgacgtgacc
      121 catgatcgtg acgatcgggg gtcgcgacgg ggagtcgtcg tcttcgtcgt cctcgccgag
      181 cagctcgtcg acgccgagac gcttctgcag ctcgacctcg gcctcctcgc ccgggttgac
      241 caggcggatg gtggcgccga gctcagcggc gaacagctcg atcatgtcgt cggacagcga
      301 ctgcgtggcc gtgaccatct cgccctgctc caggaggaag cggacgacgt cggacgccgt
      361 gcggttcagc ttggggccga tgtcctgggg cgtggaggcg cgctcgatga cgacctcgtg
      421 gtccgggatc ggtgcgtcat ggggcgtgaa gctgggagcc tcgatgggct gcagctcgtc
      481 gcggttgcgg cgacgacgac cgccacggcg cttcggtggg cggcgaccgc caccgggacc
      541 gggacgacca ccaccggggc ctccacctgg accgccgccg gggcgggggc cgccggtgtt
      601 ggcgggcggg ggcgtgtagc ggacctgacc ggccgcgcca cggcgggcgg gacggttgcc
      661 cgggccgctc ggggccgggg ccggaccgcg gccaccagcg ccgggaggcg gcgggatcgg
      721 cttgccgcca cgggagatca tgggacccgg gggcggcggg atcggcttgc ctgacgccga
      781 cttgggcggc gtcttggaac cgggcttggc gccggggtcg ttggcggcgg ccggcgggcg
      841 gggcgcgtcg gacgcggccg gggattccgg tgcctcgggc ggtgccggcg gtgcggccgg
      901 gggcggggtg ggcgcggccg acggcggctt gggggcctcg gacggcggct tcggtgccga
      961 tgcggcaggg gcctcggccg ggggctcggg cgcagcagcc gggggcttgg gggcctcgga
     1021 cggcggcttg ggtgcggcag gcggggccgg gggcgccgca gccgggggct gaggcggagc
     1081 gggcgcggca gctgccggct cgtcttcctt ggggcggggc ggggggtgct cgctgccgct
     1141 cgacttcacg acgcccttgg tggggcgcac ggcgggcggg cgaggcatgg gcgcgtcggc
     1201 ccggccggcc tgggccgccg ccggagcgga agcaggaggc ggggtgggcg cagcaggtgc
     1261 ggacggctcg gcagcggcgg gctggtcggc ctcgggcgcc ggcttgggct tgggcggcgc
     1321 agccttcttg gcgaccacct tcttggccgg ggcagccttc ttggtcgcgg gggcctcggc
     1381 ctccgcggca ggcttgccgt cgccatcgcc ggtgtcgggc ttgggctcgc gcaccaatcc
     1441 ctcacgctcc gccttgcggc ggacgcgatc agcctgcggc tccacgatgc tcgaggagtg
     1501 gctcttgacg ccgatcccga gcgagttgca caggtcgacg gtctctttgt tctcgagtcc
     1561 gagctcacgt gcgagctcgt acaccctgat ctttcctgcc aaaagtcttc gtttcgcctt
     1621 gcgttacgtc agtagggatc tgctgcgcga ccggtcatcc tcgcgcgttc tgctctgggt
     1681 cacgaaccga gtcgacggca tctcgcaccg cggacccgac cgaagcgtga tcgaccggac
     1741 cccggaaggc tcgttcgaac gcccggcggc gcagcgccag cgccgcgcac tcggagagcg
     1801 tcgcgatgca cagccatgcg ccccgaccag ggagtgtacg accgaactcg accgttccct
     1861 cctggcttcg gaccatgcgg accagctcgt cggtgccagc agaccgctgg caaccgatgc
     1921 aggtccgcgc cggggagctc aggtcggctc ggatgccggg tcgtcggcag cccctccgag
     1981 ctcgggtccc tcgaccacgg gttccgtggt gaggacctgc tcctcggcgg catcggacgg
     2041 cacatcgccg gccgggacgt cgggctggac ccgctcgacc gggtcgtcgc cgacctcggc
     2101 ctggacggcg tcatcggcgg cggcggccag atcggcggcc ggatcgagcg gctcgacgac
     2161 gtcttcgtcg tcggccggcg cgacatcggc gtcctcggtg acctgggtgt cgtcggagtc
     2221 cggggcggcc tcggccacgg ccggctcctc gacctcgtcc tcttcgcctt ccatgtactc
     2281 ggcggcggag acggcggggc caccttcggc gggctgccag acctgctcgc cggtctcctc
     2341 gtcgaccacc cattcaccct cggcccagtc ctcggcggcg taggccgctt cggcctcggc
     2401 gagctgggtc tcggacttga tgtcgatggc ccacccggta aggcggccgg cgaggcgggc
     2461 gttctggccc tccttgccga tggccaggct cagctggtgg tcggggacga tgaccatggc
     2521 ggtgccggtg ttctcgtcga tgcggacctc cttgacccgg gcgggctgga gggccttggc
     2581 c
//
LOCUS       DATISU010000335         3430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_350114, whole genome shotgun sequence.
ACCESSION   DATISU010000335 DATISU010000000
VERSION     DATISU010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350114"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /gene="ligA"
                     /locus_tag="VLR27_13195"
     CDS             complement(<1..1070)
                     /gene="ligA"
                     /locus_tag="VLR27_13195"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HSP04458.1"
                     /translation="MSDQDVPEEQRRAEQLRAEILEHNERYYVQDAPIVTDAEYDELI
                     RELRELEAAHPELQDDTSPTQQVGGRASAAFSPVTHSSPLLSLDNAFAEDDLRSWDER
                     LQRRLGVAEGDDPITYGYVCELKIDGVAISLRYEDGALVQAATRGDGRVGEDVTANIR
                     TIADIPDRLPKGAPAVLEVRGEVYMTTAAFEALNEWAAEHGHAPFINPRNSAAGALRQ
                     KDPTITGQRELSMWSYGLGEVQGGPALARHEEMFDLLRELGFPVNPEIRTVETIDDVV
                     RLCLDREQHRHDLPYEIDGVVVKVDDLARRADLGFTSRAPRWAIAYKFPPEERTTTLL
                     DIMVSVGRTGRATPYAVLEPVFV"
     gene            complement(1117..2124)
                     /locus_tag="VLR27_13200"
     CDS             complement(1117..2124)
                     /locus_tag="VLR27_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04459.1"
                     /translation="MKAINLRVGTASAIGSMPHRQVDAAVAMAMAACADLPAAPSLPA
                     RDAREGMVAQGAWGLRGVDIGTDGVIRLVGDLDPVDPFGGEPTLEGPAFAGLRAFLDA
                     NRHRTSAIKLQLTGPVTLGLALIEAGADPDVAFATARTAVTARARSMLSTALRIAPLA
                     PQVAFVDEPSLVALSGPDFPLRTDDALDLVSSALAAVEPFAVTGLHCCGDTDWRLALA
                     TGPQIVSMPIGPAIADHAGALGVHLDRGGWVAWGAVPTSRPVGDSSDRLWRALSDTWC
                     DLVQRGCDALRLREQALVTPECGLAAHDTLQAEHILALTVDLAERVRRQSFGVRLSVG
                     A"
     gene            2245..3132
                     /locus_tag="VLR27_13205"
     CDS             2245..3132
                     /locus_tag="VLR27_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04460.1"
                     /translation="MLRSLPLAAPWPFRALWLILPVVAGPAVADALADRSRAVQVVAS
                     VLAWACWGGVLAASVVLRSVTLTVVRALAPGSVPLCTWAAFASDELAWGLAGIVVAVL
                     ATVAALSPATADAFVDGSSYGDERRVALRVPVAMAVGPVPIAWALAAAGLVVGPLLLA
                     AEQWVAGGIALVVGLPVVVAVALRLHVLSRRWLVFVPAGVVVHDPLAMAEPVLLQRHL
                     LRRIGPADADAAHDGSLDLTGGALGLALELRTVEPFEVGLRQGRETVETTGVPAVLVT
                     PGRPATTLEIAAERKLPVA"
     gene            complement(3257..3427)
                     /locus_tag="VLR27_13210"
     CDS             complement(3257..3427)
                     /locus_tag="VLR27_13210"
                     /inference="COORDINATES: protein motif:HMM:NF042466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HSP04461.1"
                     /translation="MLAQCSAHGTPHPATVTPGPGGATVTFDEPQRRLAPGQSVVLYR
                     GDLVVGGGIAQR"
BASE COUNT          406 a         1224 c         1324 g          476 t
ORIGIN      
        1 acgaacaccg gctcgagcac cgcgtacggc gtggcgcggc cggtgcgccc gaccgagacc
       61 atgatgtcga gcagcgtggt ggtgcgctcc tcgggcggga acttgtaggc gatggcccag
      121 cggggtgccc gggacgtgaa cccgaggtcg gcccggcggg ccaggtcgtc caccttcacc
      181 acgacgccgt cgatctcgta gggcaggtcg tggcggtgct gctctcggtc gaggcagagc
      241 cggaccacgt cgtcgatcgt ctcgaccgtg cggatctcgg ggttcaccgg gaagccgagc
      301 tcgcgcagga gatcgaacat ctcctcgtgg cgggccaggg ccggaccgcc ctgcacctcg
      361 ccgaggccgt agctccacat cgacagctcg cgctggccgg tgatggtggg gtccttctgg
      421 cgcagggcgc cggcagcgga gttgcggggg ttgatgaacg gggcgtggcc gtgctcggcc
      481 gcccactcgt tcagcgcctc gaacgccgcg gtggtcatgt acacctcgcc ccgcacctcg
      541 agcaccgcag gcgcgccctt cggcagccgg tcggggatgt cggcgatggt gcggatgttg
      601 gcggtgacgt cctcccccac ccggccgtcg ccgcgggtgg cggcctggac cagggcgccg
      661 tcctcgtagc gcagcgagat ggcgacgccg tcgatcttga gctcgcacac gtagccgtag
      721 gtgatgggat cgtcgccctc ggcgaccccc agccggcgct ggaggcgctc gtcccaggag
      781 cgcaggtcgt cctcggcgaa ggcgttgtcg agcgacagca aggggctgga gtgcgtgacc
      841 ggcgagaacg cggccgacgc ccgtccgccg acctgctggg tgggcgaggt gtcgtcctgg
      901 agctcgggat gggcggcctc gagctcgcgc agctcgcgga tgagctcgtc gtactcggcg
      961 tcggtgacga tgggcgcgtc ctggacgtag tagcgctcgt tgtgctcgag gatctcggcc
     1021 cgcagctgct cggcccggcg ctgctcctcg gggacgtcct ggtcgctcac cccggcgacg
     1081 ttacgcggag gtgctgacag gaaccgccag gtggtcctac gcgccgacgg acaggcgcac
     1141 gccgaaggac tggcgtcgca cccgctcggc caggtcgacc gtgagggcga ggatgtgctc
     1201 ggcctgcagg gtgtcgtgcg cggccaggcc gcactcgggc gtcaccagcg cctgctcgcg
     1261 gaggcgcagg gcgtcgcagc cccgctggac caggtcgcac caggtgtccg acagcgcccg
     1321 ccacagccgg tcggaggagt cgccgacggg acgggacgtg ggcaccgcac cccaggcgac
     1381 ccagccgcca cggtcgaggt ggacgccgag tgcgccggcg tggtcggcga tggccggccc
     1441 gatcggcatc gacacgatct gggggccggt ggccagcgcc aggcgccagt cggtgtcgcc
     1501 gcagcagtgg aggccggtga cggcgaaggg ctccaccgcg gcgagggcgc tggacacgag
     1561 gtcgagggcg tcgtcggtgc ggagcgggaa gtcgggcccg gacagggcga ccagcgacgg
     1621 ctcgtcgacg aacgcgacct ggggcgccag cggcgcgatg cgcagggcgg tcgagagcat
     1681 cgagcgggcc cgggcggtga cggccgtgcg ggcggtggcg aaggcgacgt cggggtcggc
     1741 gccggcctcg atcagggcga ggccgagggt gaccggaccg gtgagctgca gcttgatcgc
     1801 cgaggtgcgg tggcggttgg cgtccaggaa ggcccgcagc ccggcgaagg cgggaccctc
     1861 gagggtgggc tcgccgccga acgggtcgac cgggtcgagg tcgccgacga ggcggatcac
     1921 gccgtcggtg ccgatgtcga cgccgcgcag gccccacgct ccctgggcga ccatgccctc
     1981 ccgggcgtcg cgcgccggca gggagggcgc cgcgggcagg tcggcgcagg ccgccatggc
     2041 catcgccacg gccgcgtcga cctgtcggtg gggcatgctg ccgatggccg acgccgtgcc
     2101 gactcggagg ttgatcgcct tcatgaccgc tcgttccgcc tcgctgaacc gtggccggca
     2161 gggtggctga cgccaccatc cccctggttc cacatgacac cggaatagcc accgccgggc
     2221 tccccgggca aatctgtcgg ggccatgctc cgctccctcc ccctcgccgc accgtggccg
     2281 ttccgagcgc tgtggctgat cctgcccgtg gtggccggcc ccgccgtcgc cgacgcgctg
     2341 gccgatcgca gccgcgccgt gcaggtcgtg gcgtcggtgc tggcatgggc gtgctggggt
     2401 ggcgtgctcg ccgcatcggt cgtcctgcgc tccgtcaccc tcaccgtcgt ccgggccctc
     2461 gcgcccggtt cggtcccgct gtgcacgtgg gcggccttcg cctccgacga gctcgcatgg
     2521 ggcctcgccg gcatcgtggt cgctgtcctc gccacggtcg cggccctctc gccggccacg
     2581 gccgacgcct tcgtcgacgg ctccagctac ggcgacgagc gccgggtcgc cctgcgggtc
     2641 ccggtcgcca tggccgtcgg acccgtgccc atcgcctggg cgctggccgc cgccgggctc
     2701 gtggtcggac cgctcctgct cgcggccgag cagtgggtcg ccggcgggat cgcgctcgtc
     2761 gtcggcctgc cggtcgtcgt cgctgtcgcc cttcggttgc acgtgctcag ccggcgctgg
     2821 ctggtgttcg tccccgccgg cgtcgtggtg cacgacccgc tcgccatggc cgaaccggtg
     2881 ctgctgcagc gtcacctgct gcgtcgcatc ggacccgccg acgccgacgc cgcgcacgac
     2941 gggtcgctcg acctcaccgg cggggcgctc gggctcgccc tcgagctgcg caccgtcgag
     3001 ccgttcgagg tcggcctgcg ccagggacgc gagacggtgg agaccaccgg cgtccccgcc
     3061 gtgctcgtga caccgggccg cccggccacc acgctggaga tcgccgccga gcgcaagctc
     3121 cccgtcgcct gagcgacgtt cctcacgcgc agatcgacca tgccccgtcg atccgcgcgc
     3181 aacgaacgat gaggggaccg ttcctcacgc gccgatcgac catccacggt cgatgtgcgc
     3241 gctgggaacg gtttggtcag cgctgggcga tgccgccgcc gacgaccagg tcgccgcggt
     3301 agagcacgac cgactggccc ggggcgagcc ggcgctgggg ctcgtcgaag gtgacggtgg
     3361 cgccgccggg gccgggcgtg accgtggccg ggtgcggggt gccgtgggcg ctgcactggg
     3421 ccagcacggc
//
LOCUS       DATISU010000336         9532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_397293, whole genome shotgun sequence.
ACCESSION   DATISU010000336 DATISU010000000
VERSION     DATISU010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9532
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397293"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..638)
                     /locus_tag="VLR27_13215"
     CDS             complement(198..638)
                     /locus_tag="VLR27_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSP04462.1"
                     /translation="MLLRFDPFRELDRLADELDRSVARTATAPLPMDAFRRGNHVVAQ
                     LDVPGVRPDDVDITVERNVLTVRATRNVEVGEDDERIVAERRHGELVRQLQLGESLDP
                     QRVEADVTDGVLTLRIPVAETATPRKVEVGTGGGHDAGQAALSA"
     gene            786..1055
                     /locus_tag="VLR27_13220"
     CDS             786..1055
                     /locus_tag="VLR27_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04463.1"
                     /translation="MDAHERLATRVADEQDDILGTIQLVDGDDALQDRLFDQLVDLLV
                     EGMFLDLREAYLAGSIPREAYVDQLSDLAERCRSWGLLPLPSRPT"
     gene            complement(1152..2951)
                     /locus_tag="VLR27_13225"
     CDS             complement(1152..2951)
                     /locus_tag="VLR27_13225"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HSP04464.1"
                     /translation="MAATTTNERLKAWVDEWAEIFQPDAIEWCDGSDAEWDRLTSLLV
                     EQGSFTRLDDSKRPNSFLCNSDPADVARVEERTFINSVEEIDAGPTNNWRAPSEMKEE
                     VLSFYRGAMRGRTMYVIPFSMGPLGSPIAHIGVQLTDSAYVVVNMKIMTRMGQGALDV
                     LGDGDWVKCLHSVGYPLVDDEGNRRDDVAWPCDADNKYICHFPEDREIWSYGSGYGGN
                     ALLGKKCFALRIASTMARDEGWMAEHMLIIGVTPPGGEKKYVAAAFPSACGKTNMAML
                     ESTLPGWKVETVGDDIAWLKFGDDGQLYAINPESGFFGVAPGTSEETNHNALRSVDAN
                     SIFTNVAKTDDDDVWWEGLSEPPAHLIDWKGSDWTPESDAPAAHPNARFTAPASQCPV
                     IAPEFEDPKGVPISAILFGGRRATNVPLVTEAESWEDGVFLGSIMSSEKTAAAAGKVG
                     EVRFDPFAMLPFIGYNAGDYMQHWLDIGEREGAQLPKLFWVNWFRRGEDGSFLWPGFG
                     DNIRVLKWVLERLDGTADAVQTPIGLVPTAESLDLEGLDIDAAVLDELLEVHPESWRQ
                     EVPLIEDHFDSIGERLPETLRGRLETLEKKLSQ"
     gene            complement(3022..3231)
                     /locus_tag="VLR27_13230"
     CDS             complement(3022..3231)
                     /locus_tag="VLR27_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04465.1"
                     /translation="MIIADSDLNEYDLAPGALHGSVFRMLRQGQRVVFTTDADGRAAD
                     LLLGSEVDMGTPRFPQDDVPLETGI"
     gene            3418..5085
                     /locus_tag="VLR27_13235"
     CDS             3418..5085
                     /locus_tag="VLR27_13235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HSP04466.1"
                     /translation="MTTLDRLGAAELGRAIATFGDALSMHQDAINRLNVYPVPDGDTG
                     TNMTLTLRSVAEAVAGVEPEEMAAVCKAIAHGSLMGARGNSGVILSQLLRGMTEVVRE
                     HDALDGEVLAAALTRASELSYEAVQRPVEGTILTVVREAAEAARDACAGGTALVEVLA
                     RTRDAGQVSLDGTPDLLPVLKEAGVVDAGGAGFLLLLDALLHVVDGREVPPPPDAPEG
                     AADRVRGGGDVTPETGKHVSELRYEVMYLLEAPDEAMPAFREVWAGIGDSIVVVGGDG
                     IFNCHIHTDDIGGAIEAAIEIGRPRQIRVTDLLDELHEIEEERWVREAAAGADADAGP
                     EPEPVPCGVVAVATGEGIRRIFGNLRVQQVVTGGQSMNPSTAQLLEAVEAVPAPEVVI
                     LPNNKNIIAVAEQVDALTDKTVRVLPTRSITEGFAALLAYDPQSTAEANVAEMRQHAE
                     AVVNGEITRAVRDSSCDAGPIAEGDWLGISRTGIQVVSPELDDAACQLLDRLLDDHEL
                     VTIIEGDGASPGCTRHITQWLEEHHPDVEAEVHHGGQPLYPYLFGIE"
     gene            5137..5760
                     /locus_tag="VLR27_13240"
     CDS             5137..5760
                     /locus_tag="VLR27_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04467.1"
                     /translation="MELFSTIDSTFADLAKTVDQGTVAVAERLPAPVDALALRVGAVN
                     AEALRQYGRVTATTVDAVYGVVSVAWTGTAQLIDASGDALGASAGTVRRTGRRAVGDV
                     RQASSTITNRARSAAGEVQRNFSVVADRADRVEDRVEAEAEAAADKVVKATDAGIAET
                     GRTGANRPSGPYESWTKEELYERAQELDLDGRSTMSKNQLVKALRSA"
     gene            5853..7976
                     /gene="recG"
                     /locus_tag="VLR27_13245"
     CDS             5853..7976
                     /gene="recG"
                     /locus_tag="VLR27_13245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HSP04468.1"
                     /translation="MARTLRFLSGIPVSELKGVGPARAKALASVGIETVFDLLTHYPR
                     RYIDRTKEARIADLHVGEVASVLARVDRISSRRLGGGRGRGGRVMVEASVSDSSGRMK
                     ITFFNQAWREQQLAPGTEAVFWGKLEDFRGTKQLTNPVVDLVGDQTGQVVPVYPQSEK
                     ARITTQDVRRWVDEALRRSGDLVDPVPESLLDRLDLVDRTTAMRMVHVPGDSVGAYVV
                     ARKRLIFDELLRLQLALVLRKRQVERTTVGVAHDLSGALVGRFHDALPYPLTGAQRRV
                     IEEIEADLADPVPMHRLLQGDVGAGKTVVAVSAMLVAVQGGHQGALMAPTEVLAEQHA
                     LGIRELLTGLRVGDEGALFDRPLRVELLTNRVPAGERRKITEGLAAGEVDIAIGTHAL
                     LSADVDFASLGVVVVDEQHRFGVEQRAALRDKGRGTVPDVLVMTATPIPRTAAMTVYG
                     DLDVSVLDELPPGRTPIETTWARTEDDQVQVWEQVRAEVAAGRQAYVVSPLVEESEKL
                     EVSSATETYEQLSHGELAGLRLGLLHGRVAPAEKEATMRQFREGRLDVLVATTVIEVG
                     VDVPNATVMVILDADRFGIAQLHQLRGRVGRGAHRSYCHLLGEGATEDGERRLEAMVE
                     TTDGFVLAEVDLDLRGEGTILGARQKGRNDLKLASLRDHRDVVDTAREIAFELVDAPG
                     GPPEALVDEVRLFLDEDEEEFLFKS"
     gene            complement(8000..8167)
                     /locus_tag="VLR27_13250"
     CDS             complement(8000..8167)
                     /locus_tag="VLR27_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04469.1"
                     /translation="MYEPDPVITCIDCGGRAHLNAHPPEEGWQPGMLTMYRCEDCLDG
                     WYLEVPDYDDF"
     gene            8238..9326
                     /locus_tag="VLR27_13255"
     CDS             8238..9326
                     /locus_tag="VLR27_13255"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HSP04470.1"
                     /translation="MRTRPLAVLALVGSLVLAACGGGEQEAATVASTTTTEAVRKTTT
                     TTTTTEVPIPVSALTGLVLPDAFVQARPVVAVKFDNVEGRSTPQIGIGSADVVYEVPV
                     EGQVTRFLALFHSVDAAPVGPIRSARGSEIGLLEELNAPLFAWHGANALLDSHVRASS
                     VVPRSFDDVPHLYYRDPGRRMPYNSFAVGTAEIRATAPEGSTGPQQPILRFAQPGEVV
                     PSPHGVPASTVHLAFPPPFGARGGAGTAVRFEWDGARWVRFQAGHPHVDGGGSGQIGV
                     DNVIVRFTNALDSGTVDQAGSRVPTAQVVGEGEAWVFSAGKVATGTWHKPDNATPTTY
                     RDIEGNEIVLAPGKTWIALPYGPGSSFT"
     gene            9326..>9532
                     /locus_tag="VLR27_13260"
     CDS             9326..>9532
                     /locus_tag="VLR27_13260"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HSP04471.1"
                     /translation="MRVVAGSVRGRRIAAPPGTDTRPTTDRVREAMFNALGSLGAVDG
                     AVVADLFAGSGALGIEALSRGAASA"
BASE COUNT         1349 a         3466 c         3382 g         1335 t
ORIGIN      
        1 tcgtagggac cggtgacgtc ctcggcggtg acgacgccct cgatggcccg gacggcgtcg
       61 gcgacgtcgg ccgccttgcc cacctcggtc tggatgagga tgtacgcgct gacggacatg
      121 gccggagcct acgcgcgcca caggcccgcc tgcggcgggc ccgtgcgtgg tcgtcgtcac
      181 gtggtcgacg ggtccggcta cgccgagagc gcggcctgcc cggcgtcgtg gccgccaccg
      241 gtgccgacct cgaccttgcg gggcgtggcg gtctcggcca ccgggatgcg cagggtgagc
      301 accccgtcgg tgacgtcggc ctcgacgcgc tgggggtcga gggactcgcc gagctgcagc
      361 tggcgcacca gctcgccgtg gcgacgctcg gcgacgatgc gctcgtcgtc ctcgccgacc
      421 tcgacgttgc gggtggcgcg caccgtcagc acgttgcgct cgacggtgat gtcgacgtcg
      481 tcggggcgca cgccgggcac gtcgagctgg gccacgacgt ggttgccgcg ccggaaggcg
      541 tccatcggca ggggagcggt ggcggtccgc gccaccgagc ggtcgagctc gtcggcgagg
      601 cgatcgagct cacggaacgg gtcgaagcgg agcagcatct cggcctcctg tggtccatgg
      661 gtgggatctg tccgctggag agggtaacaa gaaacctgag tgttgacaac gcaactttgc
      721 tcagtcccgg aaaacgacgc tgcgtgggga aggaggtcgt ctgcggagcg gcgaacaacc
      781 ggcccatgga cgcacacgag cggttggcga cccgggtcgc cgacgagcaa gacgacatcc
      841 tcggcacgat ccagctcgtc gacggtgacg acgccctcca ggaccggctc ttcgaccagc
      901 tcgtcgacct gctcgtcgag ggcatgttcc tggacctgcg cgaggcctac ctcgccgggt
      961 cgatcccgcg ggaggcctac gtcgaccagc tcagcgacct ggccgagcgc tgccggtcgt
     1021 ggggtctgct gcccctgccc tcccggccca cctagccgag cccgcccacc acggcccgtc
     1081 cccctcgggc cggcgcgcaa gagccccagc cgaacggctg gggctctcgt cgtctccgga
     1141 tccggatggc gctactggct gagcttcttc tcgagggtct cgagccggcc ccgcagcgtc
     1201 tcgggcaggc gctcgccgat ggagtcgaag tggtcctcga tcagcgggac ctcctggcgc
     1261 cacgactcgg gatggacctc gagcagctcg tcgagcacgg ccgcgtcgat gtcgaggccc
     1321 tcgaggtcga gcgactccgc ggtgggcacg aggccgatcg gggtctggac cgcatcagcg
     1381 gtgccgtcga ggcgctcgag cacccacttg aggacccgga tgttgtcgcc gaagcccggc
     1441 cacagaaacg agccgtcctc gccccggcgg aaccagttga cccagaacag cttggggagc
     1501 tgcgcgccct cccgctcgcc gatgtcgagc cagtgctgca tgtagtcgcc ggcgttgtac
     1561 ccgatgaagg gcagcatggc gaaggggtcg aagcggacct cgccgacctt gccggcggcg
     1621 gccgccgtct tctccgagct catgatcgag ccgaggaaga cgccgtcctc ccaggactcg
     1681 gcctcggtca ccagcggcac gttggtggcg cggcgaccgc cgaagaggat ggccgagatc
     1741 ggcacgccct tggggtcctc gaactcgggg gcgatgaccg ggcactgcga cgccggggcg
     1801 gtgaagcggg cgttggggtg ggcggccggg gcgtcggact cgggcgtcca gtcgctgccc
     1861 ttccagtcga tgaggtgggc cggcggctcg gagaggccct cccaccacac gtcgtcgtcg
     1921 tcggtcttgg ccacgttggt gaagatcgag ttggcgtcca ccgagcgcag cgcgttgtgg
     1981 ttggtctcct cgctggtgcc gggggccacg ccgaagaagc cggactcggg gttgatggcg
     2041 tagagctggc cgtcgtcgcc gaacttgagc caggcgatgt cgtcgccgac cgtctccacc
     2101 ttccacccgg gcagggtgga ctcgagcatg gccatgttcg tcttgccgca cgcggacggg
     2161 aaggcggcgg ccacgtactt cttctcgccg ccgggagggg tcacgccgat gatgagcatg
     2221 tgctcggcca tccagccctc gtcgcgggcc atcgtcgagg cgatgcgcag ggcgaagcac
     2281 ttcttgccga gcagggcgtt gccgccgtag ccggagccgt aggaccagat ctcgcggtcc
     2341 tcggggaagt ggcagatgta cttgttgtcg gcgtcgcagg gccacgcgac gtcgtcgcgc
     2401 cggttgccct cgtcgtcgac gagcgggtag cccaccgagt gcaggcactt gacccagtcg
     2461 ccgtcgccga gcacgtcgag tgcgccctgg cccatgcggg tcatgatctt catgttgacc
     2521 acgacgtagg cgctgtcggt gagctgcacg ccgatgtggg cgatgggcga gccgagcggg
     2581 cccatggaga aggggatgac gtacatcgtc cggccccgca tggcgccccg gtagaaggag
     2641 agcacctcct ccttcatctc cgacggtgct cgccagttgt tggtggggcc ggcgtcgatc
     2701 tcctcgacgg agttgatgaa cgtgcgctcc tcgacccggg ccacgtcggc ggggtcggag
     2761 ttgcagagga aggagttggg gcgcttcgag tcgtcgaggc gggtgaagga gccctgctcc
     2821 accaggaggc tggtgaggcg gtcccactcc gcgtcggagc cgtcgcacca ctcgatggcg
     2881 tcgggctgga agatctccgc ccactcgtcc acccaggcct tcagcctctc gttggtcgtc
     2941 gttgcggcca tttgtctctc gcctccgtcg cgccgttgcg acaagtcgag ggatcagctc
     3001 cgagccgggt ggccaggagg gtcagatccc ggtctcaagc ggcacgtcgt cctgggggaa
     3061 gcgcggcgtg cccatgtcca cttcggagcc cagcagcagg tcggcggccc ggccgtcggc
     3121 gtcggtcgtg aacacgacgc gctggccctg ccgcagcatc cggaagaccg atccgtggag
     3181 tgcgccggga gccaggtcgt actcgttcag gtcggagtcg gcgatgatca cgccatcccc
     3241 ggtcccgggg tcgtacgact tgatgacacc ctgcacgacg gctcctgcat cgatcggtcc
     3301 aactgggtcc ggccaccata cccacgtgtc cacattccgg tcacaacgcc acggcggcaa
     3361 gaggcgaagg accctggtcc gctccggctg gtggcccatc gatagggttc cccgccgatg
     3421 acgacgctcg accgcctcgg tgccgccgag ctcggccgcg ccatcgccac cttcggcgac
     3481 gcgctgtcga tgcaccagga cgccatcaac cggctcaacg tgtaccccgt gcccgacggc
     3541 gacaccggca ccaacatgac cctgacgctg cggtcggtgg ccgaggccgt ggcgggcgtg
     3601 gagcccgagg agatggcagc ggtctgcaag gccatcgccc acgggtcgct catgggtgcc
     3661 cggggcaact caggcgtgat cctgtcccag ctcctgcggg gcatgaccga ggtcgtccgg
     3721 gagcacgacg cgctcgacgg cgaggtgctc gccgccgccc tcacgcgagc ttccgagctc
     3781 tcctacgagg ccgtgcagcg cccggtcgag ggcaccatcc tcaccgtcgt gcgggaggcc
     3841 gcggaggcgg cccgcgacgc ctgcgccggc ggcaccgcgc tggtcgaggt gctcgcccgg
     3901 acccgggacg ccgggcaggt gtccctcgac ggcacccccg acctgctgcc cgtgctgaag
     3961 gaggccgggg tggtcgacgc cgggggcgcc ggcttcctgc tcctgctcga cgccctgctc
     4021 cacgtggtcg acgggcgcga ggtccctccg ccgccggatg cccccgaggg tgccgccgac
     4081 cgggtccgcg gtggcggcga cgtgaccccg gagaccggca agcacgtgtc cgagctccgc
     4141 tacgaggtca tgtacctgct ggaggcgccc gacgaggcca tgccggcgtt ccgcgaggtg
     4201 tgggccggca tcggcgactc catcgtggtg gtcggcggcg acggcatctt caactgccac
     4261 atccacaccg acgacatcgg gggcgcgatc gaggcggcca tcgagatcgg ccggccccgc
     4321 cagatccggg tcaccgacct gctcgacgag ctgcacgaga tcgaggagga gcgctgggtc
     4381 cgcgaggccg ccgcaggtgc agacgcggac gccggccccg aacccgagcc ggtgccctgc
     4441 ggggtcgtcg ccgtggccac gggggagggc atccgccgca tcttcgggaa cctgcgggtg
     4501 cagcaggtgg tcaccggggg tcagtcgatg aacccgtcga cggcccagct gctcgaggcg
     4561 gtggaggcgg tgccggcccc cgaggtcgtc atcctcccca acaacaagaa catcatcgcg
     4621 gtggccgagc aggtcgacgc cctcactgac aagaccgtcc gggtgctgcc cacccgctcg
     4681 atcaccgagg ggttcgcagc gctgctcgcg tacgacccgc agtccacggc cgaggccaac
     4741 gtggccgaga tgcgccagca cgccgaagcc gtcgtcaacg gggagatcac ccgggcggtg
     4801 cgcgactcca gctgcgacgc cggccccatc gccgagggcg actggctcgg catcagccga
     4861 accggcatcc aggtggtgtc gccggagctc gacgacgcgg cctgccagct cctggaccgc
     4921 ctcctcgacg accacgagct ggtgaccatc atcgagggcg acggtgcgtc gccgggctgc
     4981 acccgccaca tcacccagtg gctcgaggag caccaccccg acgtcgaggc cgaggtgcac
     5041 cacggcggcc agccgctcta cccgtacctg ttcggcatcg agtgagttcc gtcacgtctt
     5101 gcatggtgga tcaagcgcac gggtaccgaa ccaagcatgg aactcttcag cacgatcgat
     5161 tcgaccttcg ccgacctggc caagacggtt gaccaaggca ccgttgccgt cgccgagcgc
     5221 ctgccggcgc cggtcgacgc actcgcgctg cgggtgggtg cggtcaacgc cgaggccctc
     5281 cgccagtacg gacgggtcac cgccaccacc gtcgacgccg tgtacggcgt cgtcagcgtg
     5341 gcctggaccg gtaccgccca gctgatcgac gccagcggcg acgccctcgg ggcatccgcc
     5401 ggcaccgttc gccggaccgg ccgccgtgcg gtgggcgacg tccgccaggc gagctcgacc
     5461 atcacgaacc gcgcccgctc cgccgccggc gaggtccagc gcaacttcag cgtcgtcgcc
     5521 gatcgcgccg accgggtcga ggaccgggtc gaggccgagg ccgaggccgc tgccgacaag
     5581 gtggtcaagg ccaccgacgc cggcatcgcc gagaccggtc gcaccggcgc caaccgcccc
     5641 tccggtccct acgagagctg gaccaaggaa gagctgtacg agcgggccca ggagctcgac
     5701 ctcgacggcc gttcgaccat gagcaagaac cagctcgtga aggccctccg ctccgcctga
     5761 tcgggcaccc aacgactccg ccaacggcgg atcccgcaac gacaccgtcg gcatcgtccg
     5821 acggtgtcgg cgcgcgcggg caagatcggg ggatggcccg cacgctgcgc ttcctgagcg
     5881 gcatccccgt ctccgagctc aagggggtcg ggccggcgcg ggcgaaggcg ctcgcctccg
     5941 tgggcatcga gaccgtgttc gacctgctga cgcactaccc gcgccggtac atcgatcgca
     6001 cgaaggaggc ccgcatcgcc gacctgcacg tcggcgaggt cgcctccgtg ctcgcccgcg
     6061 tcgaccgcat ctcgtcgcgc cggctcggcg gcggtcgcgg ccgcggcggt cgggtgatgg
     6121 tcgaggcctc cgtgtccgac agcagcggac gcatgaagat caccttcttc aaccaggcct
     6181 ggcgcgagca gcagctcgcc ccggggaccg aggcggtgtt ctggggcaag ctcgaggact
     6241 tccggggcac caagcagctc acgaaccccg tggtcgacct cgtcggcgac cagaccggcc
     6301 aggtcgtgcc ggtgtacccg cagtcggaga aggcccgcat caccacccag gacgtgcgcc
     6361 gctgggtgga cgaggcgctg cgccgctccg gtgacctggt cgacccggtg cccgaatcgc
     6421 tgctcgaccg gctcgacctg gtcgatcgca ccacggcgat gcgcatggtc cacgtccccg
     6481 gcgacagcgt cggcgcctac gtcgtcgccc gcaagcgcct catcttcgac gagctgctgc
     6541 gcctgcagct cgcgctcgtc ctgcgcaagc gccaggtgga gcgcaccacg gtcggcgtgg
     6601 cccacgacct ctccggcgcg ctggtcgggc ggttccacga cgccctcccg tacccgctca
     6661 ccggtgccca gcgccgggtc atcgaggaga tcgaggccga cctcgccgac cccgtgccca
     6721 tgcaccggct gctgcagggc gacgtcggcg cgggcaagac ggtggtcgcg gtgtcggcga
     6781 tgctggtggc ggtccagggc ggccaccagg gagcgctcat ggcccccacc gaggtgctcg
     6841 ccgagcagca cgccctcggc atccgcgagc tgctgaccgg cctgcgggtg ggcgacgagg
     6901 gagccctgtt cgaccggccg ctccgggtgg agctgctcac caaccgggtg ccggcggggg
     6961 agcggcgcaa gatcaccgag ggcctcgccg ccggcgaggt cgacatcgcc atcggcaccc
     7021 atgcgctcct gtcggccgac gtcgacttcg cgtcgctcgg cgtggtcgtc gtcgacgagc
     7081 agcaccgctt cggcgtcgag cagcgggccg cgctgcggga caaggggcgc ggcacggtcc
     7141 ccgacgtgct cgtcatgacc gccacgccga tcccgcgcac ggcggccatg accgtctacg
     7201 gcgacctcga cgtctcggtc ctcgacgagc tgccgccggg ccgcaccccg atcgagacca
     7261 cctgggcccg caccgaggac gaccaggtgc aggtgtggga gcaggtgcgg gccgaggtcg
     7321 ctgccggccg gcaggcgtac gtggtcagcc ccctcgtcga ggagtccgag aagctcgagg
     7381 tctcctcggc caccgagacc tacgagcagc tctcccacgg cgagctcgcc gggctgcggc
     7441 tcgggctcct gcacggccgg gtggcgccgg ccgagaagga ggccaccatg cgccagttcc
     7501 gggagggtcg gctcgacgtg ctggtggcca ccaccgtcat cgaggtcggg gtcgacgtgc
     7561 ccaacgccac ggtgatggtc atcctcgacg ccgaccgctt cggcatcgcc cagctccacc
     7621 agctccgggg ccgcgtgggc cgtggcgcgc accggtccta ctgccacctc ctcggcgagg
     7681 gcgccaccga ggacggcgag cgccggctcg aggccatggt cgagaccacc gacggcttcg
     7741 tcctggccga ggtcgacctc gacctgcggg gcgagggcac catcctcggc gcccgccaga
     7801 agggccgcaa cgacctcaag ctggcgtcgc tccgcgacca ccgcgacgtg gtcgacaccg
     7861 cccgggagat cgccttcgag ctggtcgacg cgcccggtgg cccgccggag gcgctcgtcg
     7921 acgaggtccg cctgttcctc gacgaggacg aggaggagtt cctcttcaag agctgacgaa
     7981 cccttcgggt tcgtccccct cagaagtcgt cgtagtcggg gacctcgagg taccagccgt
     8041 cgaggcagtc ctcgcagcgg tacatcgtca gcatccctgg ctgccagccc tcctcgggcg
     8101 ggtgcgcgtt gaggtgcgcc cgtccgccgc agtcgatgca ggtgatgacg ggatcgggct
     8161 cgtacaccgc accgaccgta tccgggcccg gtggggatgc acggcggcgg cgccggccca
     8221 tggaacactg ctcggcgatg cgcacgcgcc ccctcgccgt cctcgcgctc gtcgggtccc
     8281 tcgtgctggc cgcctgcggt ggcggggagc aggaggcggc cacggtggcc tccacgacca
     8341 ccaccgaggc cgtgcggaag accaccacga cgacgaccac cacggaggtg ccgatcccgg
     8401 tgtcggcgct caccgggctc gtcctccccg acgccttcgt ccaggcccgc cccgtggtcg
     8461 cggtgaagtt cgacaacgtg gagggcaggt cgacgcccca gatcggcatc ggctccgccg
     8521 acgtcgtcta cgaggtgccg gtcgagggcc aggtcacgcg cttcctcgcc ctgttccact
     8581 ccgtcgacgc cgccccggtc gggcccatcc gctcggcgcg cgggtcagag atcggcctgc
     8641 tcgaggagct gaacgccccg ctgttcgcgt ggcacggcgc caacgccctg ctcgacagcc
     8701 acgtccgagc gtcctccgtc gtcccccggt ccttcgacga cgtcccccac ctgtactacc
     8761 gagacccggg ccgccgcatg ccgtacaact ccttcgccgt cggcaccgcc gagatccggg
     8821 ccacggcacc cgagggatcg accgggccgc agcagcccat cctgcggttc gcccagccgg
     8881 gcgaggtcgt cccgtcgccc catggcgtcc cggcgagcac ggtccacctc gcgttcccgc
     8941 cgccgttcgg cgctcggggc ggcgccggca ccgcggtgcg cttcgagtgg gacggcgccc
     9001 gctgggtccg gttccaggcc ggccaccccc acgtggacgg cgggggcagc ggccagatcg
     9061 gcgtggacaa cgtcatcgtc cgcttcacca acgcgctcga ctccggcacc gtcgaccagg
     9121 ccggctcgcg ggtgcccacc gcgcaggtcg tcggcgaggg cgaggcgtgg gtgttcagcg
     9181 ccggcaaggt cgccaccggc acctggcaca agcccgacaa cgccaccccc acgacctacc
     9241 gggacatcga gggcaacgag atcgtcctcg cccccggcaa gacgtggatc gccctgccct
     9301 acggccccgg ctcctcgttc acgtagtgcg ggtcgtcgcc ggatcggtgc ggggccggcg
     9361 catcgccgca ccccccggga ccgacacccg ccccaccacc gaccgggtgc gcgaggccat
     9421 gttcaacgcc ctcgggagcc tcggcgcggt cgatggcgcc gtggtcgccg acctgttcgc
     9481 cggcagcggc gcgctcggca tcgaggcgct cagccgtggg gcggccagcg cg
//
LOCUS       DATISU010000337        41739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_444183, whole genome shotgun sequence.
ACCESSION   DATISU010000337 DATISU010000000
VERSION     DATISU010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 41739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41739
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444183"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VLR27_13265"
     CDS             complement(<1..449)
                     /locus_tag="VLR27_13265"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSP04472.1"
                     /translation="MVAIGADAPGFELPGTDGTDEGRRPYRLADYAGTPVVLVFYPGD
                     NTPVCTRQLNEYTAEIDAFHDVGAQVLAISPNDVPSHEDFADKQGGFGFPLLADTDKS
                     VGEAYGILGPLGFYRRSVFVIDAAGKVAYSHRAIAGLTFRPTEELVRA"
     gene            complement(449..1198)
                     /locus_tag="VLR27_13270"
     CDS             complement(449..1198)
                     /locus_tag="VLR27_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSP04473.1"
                     /translation="MSGHSKWATIKHKKAATDQKRAKVFAKLIRQVEVAAREGGGDLD
                     ANPTLRTMFQKARDNSIPLDTIERAVKRGTGDLEGVVYEQITYEGYGPNGVGMLVEVL
                     TDNRNRAAADVRNAFTKNGGSQAEPGAVSWQFDRKGVIIVPKSVAEDDLMLVALEAGA
                     EDITDEESIWQVVTPATDLPDVRQALDDAGIDVESSEVTMLPQNTVPITEADKAKQTL
                     RIVEALEDLDDVQAVYANFDMTADMMEAAAG"
     gene            complement(1199..1780)
                     /gene="pdxT"
                     /locus_tag="VLR27_13275"
     CDS             complement(1199..1780)
                     /gene="pdxT"
                     /locus_tag="VLR27_13275"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HSP04474.1"
                     /translation="MVAIPTVGVLALQGAFARHARALEACGASTVEVRRPEHLDGIDA
                     LVLPGGESSTMSMLLERGDLFDPVAKRLADGMPALGTCAGMILLSSEILDGRPDQRCF
                     SAVDISVRRNGYGRQVDSFEADLDVEGLDDPFHASFIRAPVVERTGDDVTVLAEVDGR
                     PVLCASGPVMVAAFHPELSDDLRIHRRFLEEVL"
     gene            complement(1774..2697)
                     /gene="pdxS"
                     /locus_tag="VLR27_13280"
     CDS             complement(1774..2697)
                     /gene="pdxS"
                     /locus_tag="VLR27_13280"
                     /inference="COORDINATES: protein motif:HMM:NF003215.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HSP04475.1"
                     /translation="MSSDAQDTARSHETGTFRVKRGLAEMLKGGVIMDVVTPEQAKVA
                     EDAGAVAVMALERVPADIRVDGGVARMSDPDMIDGIQAAVSIPVMAKARIGHFVEAQI
                     LQALGVDYVDESEVLTPADETHHIDKWAFTVPFVCGATNLGEALRRISEGAAMIRSKG
                     EAGTGNVVQAVRHLRSITGDIRKLTQADEPELFEWAKQLRAPLPLVQEIAETGTLPVP
                     MFCAGGLATPADAALAMQLGADAVFVGSGIFKSDEPGPRAKAIVEATTHFRDADILAK
                     VSRGLGTPMTGIGMDPAGTATDGTRFEDRGW"
     gene            complement(2776..3561)
                     /locus_tag="VLR27_13285"
     CDS             complement(2776..3561)
                     /locus_tag="VLR27_13285"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSP04476.1"
                     /translation="MATDLSTAREPTTDLARIAVVHAVVVAVPVLVLGVILLPLWLAP
                     IVAIVVAVAVTALRCRGIDERIAGALGARAIDAGDAPRLDGVVESTSMAVGVSVPQLH
                     VIEDPARNAVVWGSGTGPGASIAVTTGLLDAAERIELEGVVAHLLADVRDGVVEAPTV
                     ATALFGPLAGGPAAGLVAWLARAGEDERRVVMTDLAAAQATQYPPGLVNALERVRAGS
                     SEVERSPRPMQPLWFAAPSTPGDDDPFAAHPPLADRIDLLREL"
     gene            3779..5623
                     /locus_tag="VLR27_13290"
     CDS             3779..5623
                     /locus_tag="VLR27_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HSP04477.1"
                     /translation="MRLSRLVPVLAAGALFAASTSPAQAQTGPVDDPVEGVGTTAGAL
                     TLLGLDAGDLLTLDLLTDAASANIDEDTGPRRAAAQIAALAVESPAAGVSQVLPLLQV
                     ESTGEPDERSQAVTPIDNPVVSGTLTPLELSALVDEGGATSLLSAGLADLEVLSGILT
                     LAGTTLDLGSTALVGDASGSRGLDVDALTVLDLEALLAALGIPLTDLPLDTLLDLLDG
                     LGLLGQLEALLAPLGLDIDGLSVDAIVDLVDGLVGELDVVLGQLDEVDALVDQIASLE
                     AAEATLLADVTDCDSLSDLDDLLGVGGTLTDLCGNVNTTLTTITSQLTTLTAQLDALP
                     ELTELTALVDGLLDQLQGALEAPLDLLAGQALLSLEGLDVTVLTKATDDVATSTAEVT
                     GTLGSLQVGELPVLGTLDLTSPTDQLNGLLAQAEGLIGQVLAPIGDGALADLVQIRTL
                     EETTSVEEVAGAVVSSATFSGLDVQVAPILDQLTGLLAGLGGDDSVGALLGDLDIPLP
                     TSGATEVLQLNTLLAPVTDGLPLLAALEDGLGLQVATLSQQSTFTPIAAQAPATPGAP
                     AAPAAPAAPAPILPRTGSDDGLLLALAGAAVLAALGGRHLVRRGAADA"
     gene            complement(5642..6721)
                     /locus_tag="VLR27_13295"
     CDS             complement(5642..6721)
                     /locus_tag="VLR27_13295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSP04478.1"
                     /translation="MKVGILCPYSVTIPGGVQGQVLALARALRTKGHPTRVLAPADGV
                     PPDAGVTPLGASVPTAANGSVAPVAPDPSAQLRTMRAILDEGFDVLHLHEPLAPGPTM
                     TALLLNAVPMIGTFHAAGDSSSYRYAAPILRWGLRRLRHRVAVSPDARALAHRYLSGE
                     YELLFNGIEIDRYADGPVTAGEGRTIFFCGRHEPRKGLALLLEALQSLPSDVTVWVGS
                     DGPETERLRALYAGDRRIVWLGRISDEDKMARLRGADVFCAPALMGESFGVVLLEAMA
                     AGTAVVASDLDGYRNVATHCQDALLATVGDAGALADALRTALDDEHRRGELVDAGRAR
                     AAQFSMDLLADLYLERYERISTTRS"
     gene            complement(6718..7635)
                     /locus_tag="VLR27_13300"
     CDS             complement(6718..7635)
                     /locus_tag="VLR27_13300"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HSP04479.1"
                     /translation="MPGSRLLTAFQAASAIAGKVPPPALGPLARLGGTGAALPDATRR
                     MLVARHLQRVDPSLTGLALRRAVNRTFESYAAYWLEAFRLPTLTPAELDAGMSYEGYG
                     HLERSLHRGKGTILVLPHLGGWEWTGFWFTRVVGVPITAVVEKLEPPELFEWFTDFRT
                     RLGMHIVPLGPDAGREVIKALKRNDLVCLLSDRDIDGSGVEVEFFGERTTLPAGPATL
                     ALRTGADLVPGAMFQQPRGGHHAVVRPPMVVERRGGLRDDVARITQDIARELESLIRI
                     DPTQWHLMSPNWPSDHAALAAAGRAGGPG"
     gene            complement(7653..8339)
                     /locus_tag="VLR27_13305"
     CDS             complement(7653..8339)
                     /locus_tag="VLR27_13305"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSP04480.1"
                     /translation="MFDGRFRTSVEKGLKPVGANLRRAGIKADHLTAFGVLMSVAASV
                     AIANGALRGGLLLLALTAIPDVLDGAVAKASGTASPRGAFFDSTMDRVADAVLLGGVA
                     WYLDSTEPGRIGLLPMAVLGASLLISYERAKAEALGFDARGGLMERAERLILLGFALL
                     FDSLLIPVLWIMLVLTLITAGQRFVKVWKQASATVPQATPIDRDRRRRTLRRVERQQR
                     AARRRRRTRS"
     gene            8381..8908
                     /locus_tag="VLR27_13310"
     CDS             8381..8908
                     /locus_tag="VLR27_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04481.1"
                     /translation="MASLPTDHLSPSDAAVAVRSFPRRFRGVLARPDDTDATDDAAQA
                     DPDELARRIGPDGRSAADHLLAADGVLTLLDRALEQVRSDDDPVLHPAVRDLRDTWWE
                     DEHTPLPALLDQLEQTAERCADRIDAIPTDDWGRQARVADADATVGVLDVVREAVDVV
                     AGHLRSAERTIAAVR"
     gene            complement(8918..10516)
                     /locus_tag="VLR27_13315"
     CDS             complement(8918..10516)
                     /locus_tag="VLR27_13315"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSP04482.1"
                     /translation="MARPAADDNQLLQLGLALEAGQLGVWDWDREADTVSWSAEMEHL
                     AGMAPGAFDGTFDGFMAAVHPDDRDRVAATIAEGVAAGHHVVEHRFLHPDGTVVWVEG
                     RGSARRDEAGEVIGLVGIAIDVTDRVRYRTRLEAERPVLRALDRISLQLNRERDRDRL
                     AETVVDVAAEALGADGAGLVRRASILHPWQVVAATPGVEADVERVAHAAASTMTRAAG
                     QQVQPAPGGLAIAVVWEPDLTELLVVLHDGEVPDGAERLLRGLGAHAATALENARLHE
                     AARVELAERARLLVERDEVTRQLQRSLLPPDLPVIPNLDLAAGYLPMTQGIGGDFYDV
                     FPLHDGSWGIVLGDVCGKGPTAAGVTALARHTLRAAAMAQHRPAEAVHILNQAMTELE
                     DRTFCTVIDARLRPVDGRVCVEAAVAGHPPLLVLRADGTLEHVGPTGHLVGMFDQIKT
                     GELTVELGPGDTCVLYTDGATDVRRDGEVFGDDRLADVVHGCRGMSAAATARSIEVAV
                     VDYQRGDISDDLALVVLRVPESSR"
     gene            10582..11556
                     /locus_tag="VLR27_13320"
     CDS             10582..11556
                     /locus_tag="VLR27_13320"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HSP04483.1"
                     /translation="MVRPEPAEIIELIEARRRDEVADLVRELTANQGLDEAVGLLVEV
                     QLEVGARWQSQRWTVADEHAASAIVDHALSVACAATGAPEDGRGVVVACAEDEWHVLP
                     ARMLSEQLRSRGWDTLFLGSSAPATELGRFVAHAEPVAVGLSCSMDRNLPGARRSIEA
                     CHEAGVPVVVGGAAFARPGRVAALGADASCATLAELDELLETWSSARPVLASPTVEAP
                     PALDGLARHRVLDATLRAMVERIHPLADARPHELVRLHDDLDGLLRGAEAAAHVRDPD
                     IVADHVAWLGAVSEARGEPAGLVAVALEALAGAAPEWLDLRGELAVEV"
     gene            complement(11527..12042)
                     /locus_tag="VLR27_13325"
     CDS             complement(11527..12042)
                     /locus_tag="VLR27_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04484.1"
                     /translation="MADPRDDDVVDELPEDLDAAGYVGPYVFPDNNRRRIPGYLYLLT
                     AAACIALWALAGDDAVLVNDGILIVGVVLALVGAYHLLAGYDLEVDERDALVEASRAV
                     GFPVGHASAQMGWRGLRSRPTWRILLYSAEEPPEQRGFVLVDGVDGAIVDTLVEGNPE
                     DWSDLDGKLTA"
     gene            complement(12156..13082)
                     /locus_tag="VLR27_13330"
     CDS             complement(12156..13082)
                     /locus_tag="VLR27_13330"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HSP04485.1"
                     /translation="MQRNLRLLGALLALMLFAAACGDDDDETTTAGDESTDEQSDDAE
                     AAGGTVQIGMIPWDEDIAVTHLWTHILEEEGYTVETTQLDAAPTFQGLAAGDLDVFLD
                     AWLPSTHEDYWEEYGDELEDLGTWYDQGTLNIAVPSYVDMETIGDLAGQADMFDGQII
                     GIEPGAGLTRVTRDEAMPGYGLDGFELVEGSTPAMLAELERALDDEEPIVVTLWHPHW
                     AYAAYDIKDLEDPDGLMGGAEELHAVARSGFSDDFPEVAEWMGNFELTDEQLASLEDL
                     MFNTYESGQEAEAVAEWLEDQDNADAVQSWIS"
     gene            complement(13127..13984)
                     /locus_tag="VLR27_13335"
     CDS             complement(13127..13984)
                     /locus_tag="VLR27_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015373553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     permease"
                     /protein_id="HSP04486.1"
                     /translation="MVEIPRIPIGDRVDEGVDWLTDNLGDVFDFVSDQVRWLVDHLED
                     LLLWPPAIVLAALFALVAWRLRSLAFGIFTFLSMLLIESMTLWEESMSTLSLVLIATL
                     VAVAIGIPLGIAAASSERVSSVLRPVLDFMQTMPAFVYLIPAITFFGIGAVPGVVATV
                     VFAMPPGVRLTELGIRQVDTEVVEAAESFGAPPRQVLRKVQLPLALPTIMAGVNQVIM
                     LALSMVVIAGIVGAGGLGAEVFRGITRLDVGVGFEGGLAVVILAVFLDRTTASIGSTG
                     RRPPPAGTA"
     gene            complement(13977..15188)
                     /locus_tag="VLR27_13340"
     CDS             complement(13977..15188)
                     /locus_tag="VLR27_13340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HSP04487.1"
                     /translation="MSTIELQNVYKLFGRRADEALELLREGVPGAEVQERTGAVPAVI
                     DISLDIQQGELFVVMGLSGSGKSTLVRTINRLHEPTAGTIKLGGRDITDLGPAALREV
                     RSASISMVFQHFALFPHRTVLENAAWGLEVRRRDRSERTERSREALRMVGLEGWEDRY
                     PDQLSGGMQQRVGLARALATDADVLLMDEAFSALDPLIRREMQDQLIELQETLHKTIV
                     FITHDLNEAMRLGDRIAVLKDGRLVQVGTAEDILQDPANDYVASFVQDVDRSRVLTAG
                     SVMEKAHAVITTHDGPRAAMREMRSLQVSALLVVDAQRRLRGYVTDEDVAAAIRDGAQ
                     RLDGALRSDLTTISPDVALVELFVPAAATPLPLPVEDEHGRLVGVVPRVTLLAALGGQ
                     TVDTPPEADHG"
     gene            15456..17099
                     /locus_tag="VLR27_13345"
     CDS             15456..17099
                     /locus_tag="VLR27_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSP04488.1"
                     /translation="MGAIEVSDAGYRLPGGRPLFDDVSFRVGDGQKVAMVGANGVGKT
                     TLLRLIAATATGEPHDDHDGTIRVDGRAAVMDQLVGMRGTGGTVRDLFVSLAPPPVRA
                     AAEQLTAAERAMAGAERGGKPSFVAEAGMRLAEAHGRWGDVDGYDAEVLWEVCCTEAV
                     GLPLDVACERPLSTFSGGQQKRLALEYLLRSDAAVLLLDEPDNFLDIPGKRWLEQRLG
                     ESRKTVLFISHDRELLAASADKIVTIEGDGAWTHGGSFATYHQARADRHARLADDHRR
                     WTEERKRLVELVRVMKERAKVSDANASRARALETRLRHFDEAGPPQEQPKEQRITMRI
                     AGGRTGKRAITMEDLELIDLTFPFSAEVHYGERVGVLGANGTGKSHLLRLLGGDRTVA
                     HEGRCELGARVVPGHFSQTHDHPELHGRTLVDVLRARDLDLGASMSRLRRYDLQGAAD
                     QTFETLSGGQQARFQILLLELGGATLLLLDEPTDNLDLVSAEALEDALEAFDGTVVAV
                     THDRWFLRSLDRFLVFREDGEVEVCDEFSWARTGAASPT"
     gene            complement(17062..17619)
                     /locus_tag="VLR27_13350"
     CDS             complement(17062..17619)
                     /locus_tag="VLR27_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HSP04489.1"
                     /translation="MTTPDREGARSPLDRLWAGWRTAYMDEVTADGNAGLPTEGGSVF
                     ARILRAIEAGDLTDETAYLVHRGDHAFAILNAYPYGTGHLLVMPYREVGALEDLTGEE
                     SADVWRLVNEAVRAIKAAYRPEGVNVGFNLGRAAGAGVPTHVHGHVLPRWDADSNFMT
                     TVAEARVLPEPLSVTWEKLRQSWPS"
     gene            complement(17606..19603)
                     /gene="thrS"
                     /locus_tag="VLR27_13355"
     CDS             complement(17606..19603)
                     /gene="thrS"
                     /locus_tag="VLR27_13355"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HSP04490.1"
                     /translation="MPDQIAVTLPDGSSRELPTGSTAGDLARAIARGLAKKAVIAEVN
                     GIERDLSHELADGDAVAIVTADSDQGLYVIRHSTAHVLAQAVLELFPGATFGIGPPVE
                     NGFYYDFELPDGGTFHEEDLERIEERMREILKESQPFVKSEIGADEARQIFADHKYKL
                     EIIENASTDPMSATSERGEVRCYENIPPEPRPNQTFHGHDGFIDLCRGPHVPTTSDHL
                     GHFKLMRVAGAYWRGDEKNPQLQRIYGTAWSTKAELAEHLQRLEEAAKRDHRRLGTEL
                     DLFSFPSEIGGGLAVWHPKGGIVRKLMEDYSRARHEEGGYEFVYTPHLTKAGVFETSG
                     HLHWYADGMYPPMEMDNGTYYMKPMNCPMHCLVYRGRQRSYRELPLRLYELGTVYRYE
                     RAGTLHGLMRIRGFTQDDSHIYVAEEQLADEIAGLLDFVLSVLRAFGFEDFTFNLSTK
                     DPDKYVGSDEIWEKATEALREALDRHGLEYGVKEGDAAFYGPKIDIDVRDAIGRSWQL
                     STIQADFNHPEKFDLTYVGADNEHHRPIMLHRALFGSIERFFGVLLEHYAGAFPTWLA
                     PVQAVVCPVADEHEDHAQTVADRLREAGVRVEVSPADDGLGARIRRHKMEKVPHILVV
                     GGDDVANDTVADNVRGGDKPERDIPVADLIERITAEIRDHA"
     gene            complement(19614..20141)
                     /locus_tag="VLR27_13360"
     CDS             complement(19614..20141)
                     /locus_tag="VLR27_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSP04491.1"
                     /translation="MVVLTDQDAIDLALLVFRCAVGAVMFAHGWNHVFRGGKIEGTAG
                     WFASLGMKPGILHAWLASLAELAAGISLVLGLLTPLGAAAVVGTMTVALITNHRGNGF
                     FIFRPGEGWEYVMTLVAAGVALGVVGPGGWSLDEALDLFQPVPGSDGLWIVLAAGFGG
                     ALALLSVFWRPQRGQ"
     gene            complement(20129..21046)
                     /locus_tag="VLR27_13365"
     CDS             complement(20129..21046)
                     /locus_tag="VLR27_13365"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSP04492.1"
                     /translation="MGDEREYGKMYPVAKGLIRPLMHALWRINVTGAHNVPAEGPAIF
                     CPNHISFFDSLVLPCVLPRRISYVGKAEYMDSWKTKYIFPAIGMIPIDREGGSASQRA
                     LDTATRVLERGELFGIYPEGTRSRDGRLHRGHTGPARLALRTGAPLIPVGFRGTDRIQ
                     PAGASLPQPFRSVQVNIGAPIDVTRYRDRADDRLVLRQLIDEVMYEIRKLSGQEYVDE
                     YANKRDDERDPEASEVAAPAPVAEPAELGNGNGHPRAEPEPEPAPQRERVLVGAGGAH
                     DVEHAEDIEVHHDVDVRRSSADVLSKWWS"
     gene            complement(21092..22231)
                     /locus_tag="VLR27_13370"
     CDS             complement(21092..22231)
                     /locus_tag="VLR27_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HSP04493.1"
                     /translation="MSPLRLYDTARREIVPFEPGEIVTMYTCGITPYDATHLGHAAAY
                     MGYDVLQRRLRDRGHQTRCVRNVTDVDDSILGRAREIGVHYLDLAAAETAKFDDDMVA
                     LGMLPSWSEPRATSAIADIRGFIGMVLDRGHAYQAGGAVYFDISTYEGFGQVSGYDRD
                     TMLEYAAERGGNVDDPNKRDALDFVLWQPSLPDEPSWDSLWGPGRPGWHIECSALALR
                     ELGTTIDLHGGGADLIFPHHECEAAQSEAATGEPFVRHWMHQAMVRMDGEKMSKSLGN
                     LVFVSELRKEWDAMAIRLAVVKHHYRDGWEWHEDLMPEAAERLARWRAGDGGEGGAVL
                     ETVRAHLDEDLDTPGAVEAIDVAAAAGADVTDAALLLGVQLVPGS"
     gene            complement(22228..22962)
                     /gene="npdG"
                     /locus_tag="VLR27_13375"
     CDS             complement(22228..22962)
                     /gene="npdG"
                     /locus_tag="VLR27_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HSP04494.1"
                     /translation="MKIGILGGTGPAGSALAARLADVGYDVIIGSRSAERAKEKVEEL
                     LDKWPGRTLAIEAGDNAAAAAADVVVVATPWDGATQTAASVSDHLSGKVVISMANALT
                     RIGKEFQPLVPPRGSVAASIQAVVPDCKVAGAFHHVPAKELGDLDHPIETDVLICSDH
                     EEATAQTTELIQAIPNMRPLDAGELSQSTPIESFTAVLLQLNVRYKTRVAVKFNGLPD
                     SLTGLPKKPPRATVAAERDAGDPNSK"
     gene            23034..23471
                     /locus_tag="VLR27_13380"
     CDS             23034..23471
                     /locus_tag="VLR27_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04495.1"
                     /translation="MPDPTADPAAGPPRQLSPVPFVVALVGFVVIAALAFVFLRADDE
                     GRLVRPDRFQVLDADTITATALGQPGCGRVERAQVDLADTEVLVELVVVDVEGPCSDV
                     TVDLVVEITLPEPVGDRAVRAGVGRTRLPCTGDARSTTCGPTG"
     gene            23517..24284
                     /gene="fabI"
                     /locus_tag="VLR27_13385"
     CDS             23517..24284
                     /gene="fabI"
                     /locus_tag="VLR27_13385"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HSP04496.1"
                     /translation="MGILDGKRLLITGVLTDASLAFGVAKLAQEEGAEIVLTGAGRAL
                     RLTQRTARKLPVEADVLELDVTVPEHVHAVRDALGAKWDRVDGALHAIGFAPESCLGG
                     GFTEAPWEDVAVAMQISAYSLKTIADVVVPLMPEGGSIVGLDFDATQAWPAYDWMGVA
                     KAALESTSRYLARDLGPKNVRVNLVAAGPIRTMAAKSIPGFSAFEDVWDDRAPLGWDV
                     NDSAGVARTTVAMLSDWFPQTTGEIVHVDGGYHAIGA"
     gene            complement(24281..25630)
                     /locus_tag="VLR27_13390"
     CDS             complement(24281..25630)
                     /locus_tag="VLR27_13390"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HSP04497.1"
                     /translation="MTPDELADRLQDLLRTELADPDLEVSGLRRLTGGASRETWAFEA
                     GGDRFILQRERTGSPRTGAMATEASMLRAAADAGVPVPSLVSASGGPGDRGDLGAGYL
                     VSRFVDGETIARKILRDDEFATARDRLAADCGRALAAIHRVPTDAVPGLERTDQVAQY
                     RTVLDDFGDPHPTFEHAFRWLEANRPPSSGDAIVHGDFRLGNLMVGPDGLEAVIDWEL
                     AHIGDPMEDLGWLCTRAWRFGSEAPVAGVGPYDQLFDAYEEASGRPVDRDVVLWWEVL
                     GTLKWGIMCIMQARAHLEGLSRSMELAAIGRRVCENEHDLLLLLEPDALAAAVARPAP
                     TGGGAPGLHGRPTAAELLEAVREWVTGDVREQASGRVGFHSRVAENVLATLEREATMG
                     PAMEADHAERLQSLGVADDLAFAAGVRDGSLRGDEVVRAMAESVVDKLRVANPRWFA"
     gene            complement(25690..26796)
                     /locus_tag="VLR27_13395"
     CDS             complement(25690..26796)
                     /locus_tag="VLR27_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HSP04498.1"
                     /translation="MLHRLLAPLVVTLAVAASLAVPSAAGAQTADIQRARAAAEEAVR
                     RVSDAESALGAIERDIASLEAKATQAEERLAALSGSMEELVVERYTSSGDMPLLGTDD
                     INEGVKAEAFARYVSQGTFDTIDDFTEAKAELDEASAALAAKRQQQESSIESLRRALA
                     DVQAELKRLEELEAKRRAEEEARRKAAEAEAQRRAAAEAQAQQRTSQAARSSATTSGS
                     ASRAASPAPAPAPAAAPATSSGGGMVCPVAGPHSFIDSWGYPRSGGRSHKGVDMMART
                     GVPVVAPVSGTVSHRGNSIGGLSFHLSGDNGHYYYGTHLSAYGASGRVSAGTVIGYVG
                     DTGNARGMPHLHFEIHPNHSGPVNPYPATRAACG"
     gene            complement(26869..27957)
                     /locus_tag="VLR27_13400"
     CDS             complement(26869..27957)
                     /locus_tag="VLR27_13400"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HSP04499.1"
                     /translation="MNATALIASVLLLAANAFFVAVEFALVASRRTRLAALADEGSKA
                     AARAMDSIQDLSRQLAGAQLGITICSLLLGYVAEPAIAHALEDLLQDRIDVSEGVATT
                     VGFGLALSIVVFLHMVLGEMVPKNIAIAEPERTVLVLDWANRLYMRIFGPVIVVLNAL
                     ANGIIRLLGAEPADEISDSHTSDELADMVSASRKEGLIHDVQDQILRGALAIGEAPIG
                     PMVVPRDRIGYVPRQATTVEAEREVVRSGHTRLLVVGEGGLDDVLGFLHAKDLLTVPE
                     ALRGAPLPLSKIRRLPIVRDDTIVDDVLHALQKARVHLAIVADAHGRTVGLVSLEDAL
                     EELVGEIVDESDKPRTVRSTRTRPLPSA"
     gene            complement(27954..29291)
                     /locus_tag="VLR27_13405"
     CDS             complement(27954..29291)
                     /locus_tag="VLR27_13405"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HSP04500.1"
                     /translation="MARWTPGLCTLCPIETALGLVAVAVLITANAAFVANEFALVAVD
                     RGRVSRHAREGKGAARVTEKLLRRLSYHLSGAQLGITITSLVLGFIAEPTVAAVIEPA
                     VAAVVGESSGRGVSIAVALALATTVQMVLGELVPKSLAITYPTGTAYFLAPVMRVYGI
                     VVKPVVALFNGCANWVVHRLGIEPQEELRSVRGLEELDLLFRSSGEEGTIDEDSVNLL
                     TRAVRFSEKSAVDALVPRLETVGVAVDETVADLTARAVTTGFSRFPVYDGDLDDIVGV
                     SHVKDAFGVPYEQRGTTSVRSVMAQPLVVPETMQLSVLLVLLREQATHLAVVVDEHGG
                     TAGVITLEDVLEEIVGEIDDEHDRAAPALTRVQRPGEWVLGGGLHRDEVFDACGLELP
                     DGPFETLAGFVLDTLGRLPVEGDAFDHGDWTVTVREMDRMRIAEVVLVERSGG"
     gene            29276..30112
                     /locus_tag="VLR27_13410"
     CDS             29276..30112
                     /locus_tag="VLR27_13410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSP04501.1"
                     /translation="MSTEPPDATPAEAPDAVVELVGVDVAVDGVPVLTGLDWTIRAGE
                     RWVIVGPNGSGKTTLLRLLSSWLRPWSGRAVVLGETLGRTDMRALRRRVGYTSQALTD
                     LLRPTMTPHEVVLAAPNGALETWWHRYGDDQHTDASTQLRLFGIDEETEHRPLRTLSS
                     GERQRVLLARAFTGRPGIVILDEPTAGLDVGGREDLLRRLDAAAATTTAPLVLVTHHL
                     EEIPAAFDRVVLLADGRIAGQGPATSLLTSARLSDLYGVDLDVACRDGRWSAVAATRT
                     AD"
     gene            complement(30120..30575)
                     /locus_tag="VLR27_13415"
     CDS             complement(30120..30575)
                     /locus_tag="VLR27_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSP04502.1"
                     /translation="MDLRASLEADCTPDTLFEWVGGLDTYDRWLEIVPRTADAEAIDG
                     DPGPAWYVTLRGRLGPLARSKRLRMVRTEHTRPVGGEPGRAVFERRETDGREHAAWRL
                     TATVEPTPTGACLLMDLHYGGSLWVPLLDRVLTSEIEASRQRLRALVES"
     gene            complement(30587..31936)
                     /locus_tag="VLR27_13420"
     CDS             complement(30587..31936)
                     /locus_tag="VLR27_13420"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HSP04503.1"
                     /translation="MTRTPSTPLRVLATALAVFSVAVGAPALAQEDPPPTEPVDCSTT
                     STSTTSTTPGTPEPCPTTTTAPPPPPPPPADSSTTTTPPPPPGGEEQPPPPEETAPTT
                     PPDQAGDQDSPPESVPLSEETIPPPPGEPVDPLAAERLIRRELRVAQAEVLQSAAEVE
                     GAATLVDDLSDRLADLQQQLAHLDVAQQLKVQELEEAQVRFKERIANAVVRGNAAELD
                     TVMTSQDGTVLEMRKVFLASVSEADSQSVADLQEAKAVIDQGVLDAVDAVAQTRRELR
                     SARQALQDSLRINTERKFQLAVFSAGSEIVIRGFVFPVGEPYDFIDSWGYPRMVGTEY
                     EHGHQGVDIMAPFDTPLYAVERGIITRVGTDVLGGTKLWLKGQSGTYYYYAHLAAFAE
                     GMVEGALVNAGDVVGFVGDTGNAQGGAPHLHFQVHPNGGDAVNPYGLLRVVADLSRR"
     gene            32019..33170
                     /locus_tag="VLR27_13425"
     CDS             32019..33170
                     /locus_tag="VLR27_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP04504.1"
                     /translation="MERTLFDDEHELFRESFRRFVADEITPNLEQWEADGIADRSLFS
                     KAGSHGFLGMAVPEEHGGGGVDDFRYNQVVIEEVQAAGAGGAGLGITLHNDICLPYFL
                     TLTNDEQKARWLPGIASGELITAIAMTEPAIGSDLASMTTSAVRDGDDWIVNGSKTFI
                     TNGINADLIIVAVKTDPSQKHAGMSLLVVERGMDGFERGRNLDKIGMHSQDTAELFFN
                     DVRVPAANLIGDEGKGFLHLVDKLPQERLSIAVAGVAAAKAGLDWTLDYVKERTAFGQ
                     PIGKFQNTRFTLAEVATEVEIGQAYIDKCVLALNEGTLTAEEASMAKWWCTELQHRAL
                     HECLQLFGGYGFMTEYPIARAYADARVTTIYGGTTEIMKEIISKRHLGL"
     gene            complement(33177..34130)
                     /locus_tag="VLR27_13430"
     CDS             complement(33177..34130)
                     /locus_tag="VLR27_13430"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HSP04505.1"
                     /translation="MTTPRVVCCGEALVDLIGADASGRTWHAAPGGSPFNAAVAAGRL
                     GAPTSFLGVLSDDRFGQLLRDHLDASHVSWRTCPITTEPTTLAVVTQGPDDLEPSFAF
                     HVAGTTTVSARTIDLHLPADTGVLHVSGSMALVLEPAASRLEALLAAARHRTLVHLDP
                     NPRPAVAGGRERYLARLDRWLGLADVVKVSVADIDWIEPGANPLDLAQRWLRDDDGDV
                     PAAIVITRGAEGAAVVCSAGVIEVVPAAPVDVVDTVGAGDTFVGAMLAAFAAHGITDR
                     RALADLDEHWWHTALTFAVEAAGIACGRIGADPPWRRELQH"
     gene            34218..35915
                     /locus_tag="VLR27_13435"
     CDS             34218..35915
                     /locus_tag="VLR27_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSP04506.1"
                     /translation="MKNTTSTRGWLRALALVLGLAMVAAACGGGDETSTDTGDATTGT
                     GGVEDSIPDAPEGDPTPGGELTYALEAETNGGFCLAEAQLAISGMQVAATIYDTLTKP
                     DADGQIQPHLAESVEPNEDFTSWTITLPEGVTFHDGTPLDAQVVANNIDAYRGAYENR
                     SPLLFRFVFQDVASVQVVDPQTVQVDMTRPWSAFDWALYGSNRIGIMGQAQLDSEDCG
                     TELVGTGPFQLESWTVGEELVATKNPSYWRTDADGNQLPYLDTITYQPIPEVASRVNA
                     LEAGQVDVLHTSDTEQIAQRLQPLAEQDQIQLLATDSYGEVNYLMLNTDAAPFDNKDA
                     RLALAHALDRQLLIDVRGSGLGSVADGPFPEDVPGHVADPGFPQYDPAKAQEHAAAYE
                     EATGEPLSFSYTFVSTESGQLTAQEVQTQLADAGIEISLSPAGDQATTINKALGGDFQ
                     AMAFRNHPGTDPDRENNWWYTGSPVNFGRIADPEVDRLLDEGRETAPGEERDAIFQDL
                     TRRFAEEVYNVWLSTAVWAIATQPDVHNVLGYGPEAGSEAFPGVATGHDVAGIWVSR"
     gene            35999..36616
                     /locus_tag="VLR27_13440"
     CDS             35999..36616
                     /locus_tag="VLR27_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HSP04507.1"
                     /translation="MLPPPRLTRLVRCVLGLVLLGVGLGLVVAADLGAAPWDVLHQGL
                     SQRLGITIGTASIGVGIVILLAWIPLREKPGVGTVLNVFVIGATMDLTLAVVDRPDAL
                     WVQVAFLVLGVWLWGPGSGLYIGSGLGSGPRDGVMTGLARRGLRVGRVRSAIEVVALS
                     VGFLLGGTVGIGTLVFAIGVGPNVAWWLPRLDMRAARPAALARCG"
     gene            complement(36585..37850)
                     /locus_tag="VLR27_13445"
     CDS             complement(36585..37850)
                     /locus_tag="VLR27_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP04508.1"
                     /translation="MIQWTEEQQMVRDAVRQFVEAEVAPRRDELEFGDTPPYDVLRKL
                     YSTFGMDTMARERFKRQIEFEKSVEEAIAKGEEPPTKEKAGGEGGGGGGDGSITMIPI
                     IELCRYSPGLVTAMGVSTGLTASAITSKGTIRQKERWALDLLTLDKIGAWAITEPGSG
                     SDAFGSMKATARRDGDEYVLNGSKTFITNGPYADTIVFICKLDEGNPPEERKVLSFVL
                     DSGMPGLEQSKPLRKMGMHSSPTGELFLDDVRVGLDRLIGETEDMPKGGREGAKATFL
                     MERAGVAAMALGMIEKCLELSVEYAKDRVQFGQPIGQFQLIQEKLARMEVARLNVQNL
                     VFRTIESQAAAARGEEMAMTLAEASAMKLYSARAATEVALEAVQIFGGNGYMSEYQVE
                     QLARDAKVLQIYAGTDEIQITHIARALLA"
     gene            complement(37847..38533)
                     /locus_tag="VLR27_13450"
     CDS             complement(37847..38533)
                     /locus_tag="VLR27_13450"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HSP04509.1"
                     /translation="MAEALEGPLTPIQQRVLGALAEKAATVPDTYPMTLRGLQTACNQ
                     KNNREPVTEHTEGEIQAALDELKARHLVRFVYASHGARSTKFRHVLDERLGLDAGELA
                     LVTALLLRGPQTLNELRTRTERAHAFDSNDEVAATLEGLAARTDPVVVHLPRQPGQKE
                     TRWAHLLGDGPDERAVEHTSEAPVPVPVPAPAGLDARVAELEERVAALEALLTDPTDP
                     ARTTEETSPT"
     gene            38609..39922
                     /locus_tag="VLR27_13455"
     CDS             38609..39922
                     /locus_tag="VLR27_13455"
                     /inference="COORDINATES: protein motif:HMM:NF013153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006952 - defense response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neocarzinostatin apoprotein domain-containing
                     protein"
                     /protein_id="HSP04510.1"
                     /translation="MTEETEQVIDLAPAGASIGTDAPRSRAVLVALAVVAALAAAVLV
                     LVPGDDAEVAVDEDGETETETEPEPDGRDATAFPAPLALGAPDDGKASVGLPVKAEPA
                     TGLVDGQTVQVTGTGFPPGESIGVVMCTKEAGQDHGARGVDACNIGRFAQTTADADGV
                     ATVGFEVRRLVVLDGQEVDCASEAQRCLIGMGMISDYDQSGGVLVDFDPSVPLPDPPT
                     SSIEAPGPFDDGEAVTVRVDGLRPGTGVGGTVCTADGAFCTDAPSNGVVADDGTATFE
                     LRLWRVFSAPVWEATTAGQDIDCAVVTCRLQLWGEAVGGRAIPMLDLSFTDGPIDRVR
                     PVLEVRSEGPYRPGDRIEAFVPDVGPNSGLELLLCGPQACGGGSVELDWVFGGIQARL
                     TVPSAGEGNPCIGEPCRLVAQVWAEPVPGAAPPLAPAPVEVVVEA"
     gene            40011..40490
                     /locus_tag="VLR27_13460"
     CDS             40011..40490
                     /locus_tag="VLR27_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HSP04511.1"
                     /translation="MTFKVGDKVVYPHHGAAVIEKTEVMESFGEEKEFFVLRMTHGEL
                     TLKVPSDRAEEIGMRYPISDEDVEDVFEVLAKKDVREPTNWSRRFKNHQEKLKSGDVY
                     QVAEVVRNLALREADKGLSAGEKSMFVKARQVLVSELAFALDIEEEDALARLETTLS"
     gene            40497..41660
                     /locus_tag="VLR27_13465"
     CDS             40497..41660
                     /locus_tag="VLR27_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04512.1"
                     /translation="MPGDEETEETGPSDETIARLRSRAEQLQAARRLREQDEAEHDTE
                     PDAGVPALEDEDEDDEDPFAAMLSAGAPLAGAEDLVGGADPLTEHGGDPDAERLLDRW
                     AEEERDEAVEPKRNWSPLIAVVVVLAVAALAFAGARALITGSADDDTATGGLLDTDDD
                     GSSVLDEDTPSLEDLTSDVTLPPGPDAGLQVADQGITIVPDRFDESRREGTFAVIVQN
                     PHDDWLAQGVQVSATFLDEGGQPLGADDGFVEVVLPGQSVAVASLFFDAPTVPVAGLD
                     VQVEVARWRETGPVAGGFSITEVQTNEAEFSGVRTTFLIQSDFDEPLTDVGVTAVYRT
                     ADGRIIGGYDTFVDRLDPGVATLAEIDLLANVDPAQIASTELYPTAAFGSVPD"
BASE COUNT         5730 a        14860 c        15279 g         5870 t
ORIGIN      
        1 gcgcgaacca gctcctcggt ggggcggaac gtgagcccgg cgatggcccg gtgggagtag
       61 gccaccttgc cggcggcgtc gatcacgaac accgagcgcc ggtagaagcc gagcgggccg
      121 aggatgccgt aggcctcccc gacggatttg tcggtgtcgg cgagcagggg gaagccgaag
      181 ccgccctgct tgtcggcgaa gtcctcgtgg ctgggcacgt cgttcgggga gatggccagc
      241 acctgggcac cgacgtcgtg gaacgcgtcg atctccgcgg tgtactcgtt cagctggcgg
      301 gtgcacacgg gcgtgttgtc gcccgggtag aagacgagca cgacgggcgt gccggcgtag
      361 tcggcgagcc ggtacgggcg gcgcccctcg tcggtgccgt cggtgcccgg gagctcgaag
      421 cccggggcgt cggcgccgat ggccaccatc agccggccgc cgcttccatc atgtcggccg
      481 tcatgtcgaa gttggcgtag acggcctgga cgtcgtcgag gtcctcgagt gcctcgacga
      541 tgcgcaaggt ctgcttggcc ttgtcggcct cggtgatcgg cacggtgttc tgcgggagca
      601 tcgtgacctc ggagctctcg acgtcgatgc ccgcgtcgtc gagcgcctgg cggacgtcgg
      661 gcaggtcggt ggccggcgtg accacctgcc agatcgactc ctcgtcggtg atgtcctcgg
      721 ccccggcctc gagcgccacc agcatgaggt cgtcctcggc gaccgacttc gggacgatga
      781 tcacgccctt gcggtcgaac tgccaggaca cggcaccggg ctcggcctgg gagccgccgt
      841 tcttggtgaa ggcgttgcgc acgtcggcgg cggcccggtt gcggttgtcg gtgagcacct
      901 cgacgagcat gccgaccccg ttggggccgt agccctcgta ggtgatctgc tcgtagacga
      961 cgccctcgag gtcgccggtg ccccgcttga ccgcccgctc gatggtgtcg agcgggatgg
     1021 agttgtcccg ggccttctgg aacatggtcc gcagggtcgg gttggcatcg aggtcgccgc
     1081 cgccttcgcg ggcggccacc tccacctggc ggatcagctt ggcgaagacc ttggcccgct
     1141 tctgatcggt ggcggccttc ttgtgcttga tcgtcgccca cttggaatgg ccggacatct
     1201 agaggacctc ctcgaggaat cgccggtgga tgcgcaggtc gtcggacagc tccgggtgga
     1261 acgccgcgac catcaccggg ccggatgcgc agagtacggg ccgaccgtcg acctcggcga
     1321 gcacggtcac gtcgtcgcct gtccgttcca cgacgggtgc gcggatgaag gacgcgtgga
     1381 acgggtcgtc gagaccctcc acgtcgaggt ccgcttcgaa gctgtcgacc tggcggccgt
     1441 agccgttccg gcgcaccgag atgtccacgg cgctgaagca gcgctggtcg gggcggccgt
     1501 cgaggatctc gctgctgagc aggatcatcc cggcgcaggt gccgagggcg ggcatgccgt
     1561 cggcgaggcg cttcgccacg gggtcgaaca ggtcgccgcg ctcgagcagc atcgacatcg
     1621 tcgacgactc gcccccgggc agcacgagcg cgtcgatgcc gtcgaggtgc tcgggccgcc
     1681 tgacctccac ggtgctggcg ccgcaggcct cgagggcccg ggcgtggcgc gcgaacgcgc
     1741 cctggagcgc caggacgccg accgtcggga tcgctaccag ccccggtcct cgaaccgggt
     1801 gccgtcggtg gccgtgccgg ccgggtccat gccgatgccg gtcatcgggg tgccgaggcc
     1861 acgggagacc ttggccagga tgtcggcgtc acggaagtgc gtggtggcct cgacgatggc
     1921 cttggcccgg gggccgggct cgtcggactt gaagatgccc gagcccacga acacggcgtc
     1981 ggcgccgagc tgcatggcga gggcggcgtc ggccggggtg gccaggccgc cggcgcagaa
     2041 catcggcacg ggcagggtgc cggtctcggc gatctcctgc acgaggggca gcggggcacg
     2101 gagctgcttg gcccactcga acagctcggg ctcgtcggcc tgggtgagct tgcggatgtc
     2161 gccggtgatg gagcgcagat ggcggacggc ctggacgacg ttgccggtgc cggcctcgcc
     2221 cttggagcgg atcatcgccg cgccctcgct gatgcgccgg agcgcctcgc cgaggttggt
     2281 ggcgccgcag acgaagggga cggtgaaggc ccacttgtcg atgtggtggg tctcgtcggc
     2341 cggggtgagg acctcggact cgtcgacgta gtcgaccccg agcgcctgca ggatctgggc
     2401 ctcgacgaag tggccgatgc gggccttggc catcaccggg atgctgaccg cggcctggat
     2461 gccgtcgatc atgtccgggt cggacatgcg ggcgacgccg ccgtcgacgc ggatgtcggc
     2521 cggcacgcgc tcgagcgcca tgaccgcgac ggcgccggcg tcctcggcga ccttggcctg
     2581 ctccggggtg acgacgtcca tgatgacgcc gcccttcagc atctcggcca gcccacgctt
     2641 cacgcggaac gtgccggtct cgtggctcct ggccgtgtcc tgggcgtcgc tcgacatggt
     2701 cccgagtcta cgggcgaccc ggggcggatc tcccaacgcg atcaccgcaa caggagcact
     2761 gccagaacgg aggggctaca gctcgcggag caggtcgatg cgatcggcga gcggcgggtg
     2821 ggcggcgaag ggatcgtcgt ccccaggcgt cgacggcgcc gcgaaccaga gcggctgcat
     2881 gggccgtggg ctgcgctcga cctcagagga cccagcgcgg acccgctcga gcgcgttgac
     2941 caggcccggc gggtactgcg tggcctgcgc cgccgccagg tcggtcatca cgacccgacg
     3001 ctcgtcctcg ccggcccggg ccagccaggc gaccagcccg gcggcgggac cgccggccag
     3061 tgggccgaac agcgcggtgg ccaccgtcgg cgcctcgacc acgccgtccc gcacgtcggc
     3121 gagcaggtgg gccaccacgc cctcgagctc gatgcgctcg gcagcgtcga gcaggcccgt
     3181 ggtcaccgcg atcgaggcgc ccgggccggt gcccgaaccc cacaccacgg cgttgcgcgc
     3241 cggatcctcg atgacgtgga gctgcggcac ggagacgccg accgccatcg acgtgctctc
     3301 gacgacgccg tcgaggcgtg gggcgtcacc ggcgtcgatc gctcgggcac ccagggcgcc
     3361 ggcgatgcgc tcgtcgatcc cccggcagcg cagggcggtg accgccacgg cgacgacgat
     3421 ggccacgatc ggtgccaacc agagcgggag gaggatgacg ccgaggacca gcaccggcac
     3481 ggctacgacc acggcgtgga cgacggcgat gcgagccagg tcggtggtgg gctctcgtgc
     3541 ggtggacagg tcggtggcca cggtgctccg gggacgggcg cggcgggcgc ggtgcggggt
     3601 cgggagtcta cggaccgcct ccccggcgga cgacccggcc cctgaccacg caacgtgacc
     3661 acgccgtgac tagtcagtcg ggcgtgcgaa atggcacacc tgggcgatat cgccaaagcg
     3721 gcgaatcgga caaggtggcg acgtcagtcc tgtctgttca gttggggagc ccttcttcat
     3781 gcgtctgtcc cgtctcgtcc cggtcctcgc ggccggggcc ctcttcgcgg cgagcacctc
     3841 gcccgcgcag gcccagaccg gccctgtgga cgatccggtg gagggcgtcg gcaccaccgc
     3901 aggtgccctg accctcctcg gcctcgacgc cggcgacctg ctcacgctgg acctgctcac
     3961 cgacgccgcc tcggcgaaca tcgacgagga caccggtccc cgccgcgctg ccgcccagat
     4021 cgcggcactg gccgtcgagt cccccgccgc cggcgtctcc caggtgctcc cgctgctcca
     4081 ggtggagtcc accggcgagc ccgacgagcg ctcgcaggcc gtcaccccga tcgacaaccc
     4141 ggtcgtgtcc ggcaccctga cgcccctgga gctgagcgcc ctcgtcgacg agggcggcgc
     4201 cacctcgctg ctgtcggccg gcctcgccga cctcgaggtg ctcagcggca tcctcaccct
     4261 ggccggcacc acgctggacc tcggcagcac cgcgctcgtc ggtgacgcca gcggttcgcg
     4321 cggcctcgac gtcgacgccc tcacggtgct cgacctcgag gcgctgctcg cggcgctcgg
     4381 catcccgctc accgacctgc ccctcgacac cctgctcgac ctcctcgacg gcctcggcct
     4441 gctcggccag ctcgaggccc tcctcgcccc cctcgggctg gacatcgacg gcctgagcgt
     4501 cgacgccatc gtggacctcg tcgacggcct cgtcggcgag ctcgacgtcg tgctcggcca
     4561 gctcgacgag gtcgatgcgc tcgtcgatca gatcgcctcg ctcgaggccg cggaggcgac
     4621 gctcctcgcc gacgtcaccg actgcgacag cctctcggac ctcgacgacc tgctcggcgt
     4681 cggcggcacg ctgaccgacc tctgcggcaa cgtcaacacc acgctgacga ccatcacgag
     4741 ccagctgacg acgctcacgg cgcagctcga cgcactgccc gagctgaccg agctcaccgc
     4801 cctcgtcgac ggcctgctcg accagctcca gggcgcgctc gaagcgccgc tcgacctgct
     4861 cgcgggccag gccctgctca gcctcgaggg cctcgacgtc accgtgctca ccaaggccac
     4921 cgacgacgtc gccacctcga ccgccgaggt caccggcacg ctcggcagcc tgcaggtcgg
     4981 cgagctgccg gtgctcggca ccctcgacct gacctcgccc accgaccagc tgaacggcct
     5041 gctcgcccag gccgagggcc tcatcgggca ggtgctggcg ccgatcggcg acggcgcgct
     5101 ggccgacctg gtgcagatcc gcaccctgga ggagaccacc tcggtcgaag aggtcgccgg
     5161 cgccgtggtg tcctcggcga cgttctccgg cctcgacgtg caggtcgccc cgatcctcga
     5221 ccagctcacc gggctgctcg ccggcctcgg cggtgacgac tcggtcggcg cgctcctcgg
     5281 ggacctcgac atcccgctgc cgacctccgg cgccacggag gtcctgcagc tcaacaccct
     5341 gctggccccg gtcaccgacg gcctgcccct gctcgccgcc ctcgaggacg gcctcggcct
     5401 ccaggtggcc acgctgagcc agcagtccac gttcaccccg atcgccgccc aggccccggc
     5461 caccccgggc gcacccgcag cgccggcggc ccctgccgcg ccggcaccga tcctgccccg
     5521 cacgggcagc gacgacggcc tgctcctggc cctcgccggc gccgccgtgc tcgccgccct
     5581 cggcggccgc cacctggtcc gccggggcgc cgctgacgcc tgagatcgac gcctgcgagc
     5641 gctacgagcg ggtcgtgctg atccgctcgt agcgctccag gtacaggtcg gccagcaggt
     5701 ccatggagaa ctgcgcggcg cgggcccggc cggcgtccac cagctcgcca cggcgatgtt
     5761 cgtcgtcgag cgcggtgcgc agcgcgtccg ccagcgctcc ggcgtcgccg acggtggcga
     5821 gcagcgcgtc ctggcagtgc gtggcgacgt tgcggtagcc gtcgaggtcg ctggccacga
     5881 cggcggtgcc ggcggccatc gcctcgagca gcaccacgcc gaaggactcc cccatcaggg
     5941 cgggtgcgca gaagacgtcg gcgccccgca ggcgcgccat cttgtcctca tcgctgatcc
     6001 ggcccagcca cacgatgcgc cggtcaccgg cgtagagcgc gcgcaggcgc tcggtctccg
     6061 gcccgtcgga gccgacccag acggtgacgt cggacggcag ggactggagc gcctcgagca
     6121 ggagggccaa ccccttgcgg ggttcgtggc gtccgcagaa gaagatcgtc cgcccctcgc
     6181 cggcggtgac cggaccgtcc gcgtagcgat cgatctcgat gccgttgaac agcagctcgt
     6241 actcacctga caggtagcgg tgggccaggg cgcgggcgtc gggtgagacg gcgacccggt
     6301 ggcggagccg gcgcaggccc cagcgcagga tgggtgcggc gtatcggtag gagctcgagt
     6361 cgccggcggc gtggaacgtg ccgatcatcg gcaccgcgtt gaggagcagc gccgtcatcg
     6421 tcgggccggg tgcgagcggc tcgtgcaggt ggagcacgtc gaagccctcg tcgaggatgg
     6481 cccgcatggt gcgcagctgg gccgacgggt cgggtgcgac cggggcgacc gagccgttgg
     6541 ccgcggtcgg cacgctggcg ccgagcgggg tcacgccggc gtcgggcggg acgccgtcgg
     6601 ccggcgccag cacgcgggtg gggtgcccct tggtccgcag ggcacgggcc agggccagca
     6661 cctgcccctg cacgccgccg gggatggtga cgctgtaggg gcagaggatg ccgaccttca
     6721 ccccggacct ccggcgcgtc cggccgcggc cagggcggcg tggtcgctgg gccagttcgg
     6781 ggacatgagg tgccactggg tcgggtcgat gcggatcaga gactccagct cgcgggcgat
     6841 gtcctgggtg atgcgggcga catcgtcgcg cagcccgccc cggcgctcga ccaccatggg
     6901 cgggcgcacc acggcgtggt gaccgccacg gggctgctgg aacatcgcac ccggcaccag
     6961 gtcggctccc gtgcgcagcg ccagggtggc cggtccggcc gggagcgtgg tgcgctcgcc
     7021 gaagaactcg acctccacgc cggagccgtc gatgtcgcgg tcgctgagca ggcacaccag
     7081 gtcgttgcgc ttcagcgcct tgatgacctc gcgccccgca tcggggccga gggggacgat
     7141 gtgcatgccg aggcgcgtgc ggaagtcggt gaaccactcg aacagctcgg ggggctcgag
     7201 cttctcgacg acggcggtga tcggcacgcc cacgacccgg gtgaaccaga agccggtcca
     7261 ctcccagccg cccaggtggg gcaggacgag gatcgtgccc ttgccccggt gcagcgagcg
     7321 ctcgaggtgc ccgtagccct cgtaggacat gcccgcatcc agctcggcag gcgtgagcgt
     7381 cgggagccgg aacgcctcga gccagtaggc ggcgtaggac tcgaaggtgc ggttcaccgc
     7441 ccggcgcagg gcgaggcccg tgaggctcgg atcgacccgc tggaggtggc gggccacgag
     7501 catgcgccgg gtggcgtcgg gcagcgccgc gccggtgccg ccgaggcggg caagcgggcc
     7561 gagcgcgggc ggggggacct tgccggcgat ggcggaggcc gcctggaacg cggtgaggag
     7621 gcgggacccg ggcacaggag ccagggcgcg gactacgacc gggtccggcg acgccggcgg
     7681 gcggcgcgct gctggcgctc gacccggcgc agggtgcggc gccggcggtc gcggtcgatg
     7741 ggcgtcgcct ggggcacggt ggccgacgcc tgcttccaca ccttcacgaa ccgctgcccg
     7801 gccgtgatga gggtcagcac gagcatgatc cagagcaccg ggatcagcag cgagtcgaac
     7861 agcagggcga agccgagcag gatcagccgc tcggcccgct ccatcaggcc gccccgggcg
     7921 tcgaacccca gcgcctcggc cttggcccgc tcgtaggaga tcagcaggga cgcgccgagc
     7981 accgccatcg ggagcaggcc gatgcgcccc ggttcggtgc tgtcgaggta ccaggcgacg
     8041 ccaccgagca gcacggcatc ggcgacgcgg tccatggtgg agtcgaagaa cgcgcccctc
     8101 ggcgaggcgg tgccggatgc cttggccacg gcgccgtcga gcacgtcggg gatggcggtg
     8161 agggcgagga gcagcaggcc gccccggagc gcaccgttgg cgatggccac ggacgcggcg
     8221 accgacatca gcacaccgaa cgcggtgagg tgatcggcct tgatgcccgc ccggcgcagg
     8281 ttggcgccga ccggcttcaa gcccttctcg acgctggtgc ggaagcggcc atcgaacacg
     8341 cgcaaagcgt accaagcacc cggccgatac cgtcgggacc atggcctccc tgccgaccga
     8401 ccacctgagc ccgagcgacg cagccgtggc cgtgcgctcc ttcccccgcc gcttccgcgg
     8461 ggtgctcgcc cggcccgacg acaccgacgc caccgatgac gccgcgcagg ccgatcccga
     8521 cgagctggcc cgccgcatcg ggcccgacgg ccgctcggct gccgaccacc tgctcgccgc
     8581 cgacggcgtc ctcaccctgc tcgaccgagc cctcgagcag gtgcgcagcg atgacgaccc
     8641 ggtcctgcac cccgccgtgc gggacctgcg cgacacctgg tgggaggacg agcacacgcc
     8701 gcttcccgcg ctcctcgacc agctggagca gaccgccgag cggtgcgcgg accgcatcga
     8761 cgccatcccc accgacgact gggggcgtca ggcccgcgtc gccgatgccg acgccacggt
     8821 gggggtcctc gacgtggtgc gcgaggcggt cgacgtcgtg gccgggcacc tgcgcagcgc
     8881 cgagcgcacc atcgccgcgg tgcgctgaac cgatccgcta ccgggaggac tccggcacgc
     8941 gcaggacgac gagtgcgaga tcgtcgctga tgtcaccccg ctggtagtcc accaccgcga
     9001 cctcgatcga gcgcgccgtc gccgccgccg acatgccgcg gcagccgtgc accacatcgg
     9061 ccagccggtc gtcgccgaac acctcaccgt cccggcgcac gtcggtggcc ccgtcggtgt
     9121 agaggacgca ggtgtcgccc gggccgagct cgaccgtgag ctcgccggtc ttgatctggt
     9181 cgaacatgcc caccaggtga ccggtggggc cgacgtgctc gagcgtgccg tcggcccgca
     9241 gcaccagcag gggcgggtgg ccggccacgg cggcctcgac gcacacgcgc ccgtcgacag
     9301 gtcggagccg ggcgtcgatg accgtgcaga aggtgcggtc ctcgagctcg gtcatcgcct
     9361 ggttcaggat gtggacggcc tccgccgggc ggtgctgggc catggcagcg gcccgcaggg
     9421 tgtgccgggc gagcgcggtc acgccggcgg cggtcggacc cttgccgcag acgtcgccga
     9481 ggacgatccc ccacgagccg tcgtggagcg ggaacacgtc gtagaagtcg ccgccgatcc
     9541 cctgggtcat cgggaggtag ccggcggcga ggtcgaggtt cgggatcacc ggcaggtccg
     9601 ggggcagcag gctgcgctgg agctggcggg tcacctcatc gcgctcgacg agcagacggg
     9661 cccgctcggc cagctcgacg cgcgccgcct cgtgcaggcg ggcgttctcc agcgcggtgg
     9721 cggcgtgggc gccgagcccg cggaggagcc gctcggcgcc gtcaggcacc tcgccgtcgt
     9781 ggaggacgac gagcagctcg gtgaggtccg gctcccacac gaccgcgatg gcgaggccgc
     9841 cgggcgccgg ctggacctgc tggcccgcag cccgggtcat ggtcgacgcg gccgcatgcg
     9901 cgaccctctc cacgtcggct tccacaccgg gcgtggccgc cacgacctgc caggggtgca
     9961 ggatgctcgc tcggcgcacc agaccggccc cgtcggcacc gagtgcctcg gcggcgacgt
    10021 cgaccacggt ctcggccagg cgatcgcggt cccgctcgcg gttgagctgg aggctgatcc
    10081 ggtcgagcgc ccgcagcacc ggccgttcgg cctcgagccg cgtgcggtag cggacccggt
    10141 cggtgacgtc gatggcgatg ccgaccagcc cgatgacctc gccggcctcg tcccggcgag
    10201 ccgagccccg cccctcgacc cagacgacgg tcccgtcggg atggaggaag cggtgctcga
    10261 ccacgtggtg gccggcggcc accccctcgg cgatggtggc ggcgacgcgg tcccggtcgt
    10321 cggggtggac cgcagccatg aacccgtcga acgtgccgtc gaacgcgccc ggcgccatcc
    10381 cggcgaggtg ctccatctcc gcggaccagc tcacggtgtc ggcctcgcgg tcccagtccc
    10441 acacgccgag ctgcccggcc tcgagcgcca ggccgagctg gaggagctgg ttgtcatcag
    10501 ccgcaggcct ggccacgggg cgacatccta ggaagcgcca ccccgtgtgg gacgacgcgc
    10561 cgatcccgac atactggcgc cgtggtccgg cccgagcctg cggagatcat cgagctgatc
    10621 gaagcccgtc gacgcgacga ggtcgccgac ctggtgcgcg agctgacggc aaaccagggg
    10681 ctcgacgagg ccgtcggcct gctcgtcgag gtccagctcg aggtcggggc ccgctggcag
    10741 tcgcagcgct ggaccgtcgc cgacgagcac gccgcctcag ccatcgtcga tcacgcgctc
    10801 agcgtggcgt gcgcggccac gggcgctccc gaggacgggc gcggcgtggt ggtcgcctgc
    10861 gccgaggacg agtggcacgt gctgccggct cgcatgctgt ccgagcagct ccggtcgcgg
    10921 ggatgggaca cgctcttcct cggatcgtcg gcgccggcca cggagctcgg ccgcttcgtc
    10981 gcccatgctg agcccgtcgc cgtggggctc agctgctcga tggaccggaa cctgcccgga
    11041 gctcgacgct ccatcgaggc ctgccacgag gccggcgtcc cggtggtcgt cggcggcgct
    11101 gccttcgccc ggcccggccg cgtcgcagcg ctgggggccg atgcgagctg cgcgaccctc
    11161 gcggagctgg acgagctgct cgagacgtgg tcgtcggccc gtccggtcct cgcatccccg
    11221 acggtcgagg cgcccccggc gctcgacggg ctcgcccgcc accgggtgct ggacgcgacc
    11281 ctccgggcca tggtcgagcg catccacccc ctcgccgatg cccgccccca cgagctcgtg
    11341 cggctgcacg acgacctcga cgggctcctg cgcggcgccg aggcggccgc ccacgtccgg
    11401 gatccggaca tcgtggctga ccacgtggcc tggctcgggg cggtctccga ggcccgcggc
    11461 gaaccggccg gcctggtcgc ggtggcgctc gaggcgctcg ccggcgccgc gcccgagtgg
    11521 ctcgatctac gcggtgagct tgccgtcgag gtctgaccag tcctcggggt tgccctccac
    11581 gagggtgtcg acgatggcgc cgtcgacgcc gtcgaccagc acgaagcccc gctgctcggg
    11641 cggttcttcg gccgagtaga gcaggatccg ccaggtcggg cggctgcgca ggccccgcca
    11701 gcccatctga gccgaggcgt ggccgacggg gaagccgacg gctcgggacg cctccaccag
    11761 cgcgtcgcgc tcgtcgacct cgaggtcgta gccggccagc aggtggtagg cgccgaccag
    11821 ggcgaggacc acgcccacga tgaggatgcc gtcgttgacc agcacggcgt cgtcgccggc
    11881 gagggcccac agcgcgatgc aggccgccgc ggtcagcagg tacaggtacc cggggatgcg
    11941 gcgccggttg ttgtccggga acacgtacgg gccgacgtag ccggcggcgt cgaggtcttc
    12001 gggcagctcg tcgacgacgt cgtcgtcccg gggatcggcc atggacccga cgctaccggc
    12061 gcctcgatcg ggaccccgaa cgcgcaccag cccgggagga ccggccgcgg tggccggtcc
    12121 agcccgggct ggacgtcacg gatgcgaccg gtcgatcagc tgatccagct ctggaccgcg
    12181 tcggcgttgt cctggtcctc gagccactcg gccaccgctt cggcttcctg gcccgactcg
    12241 taggtgttga acatcaggtc ctcgaggctg gcgagctgct cgtcggtgag ctcgaagttg
    12301 cccatccact cggcgacctc ggggaagtcg tcgctgaagc cgctccgggc cacggcgtgg
    12361 agctcctcgg cgccacccat gaggccgtcg ggatcctcca ggtccttgat gtcgtatgcg
    12421 gcgtaggccc agtgggggtg ccacagggtg acgacgatgg gctcctcgtc gtcgagtgcc
    12481 cgctcgagct cggcgagcat cgccggggtg ctgccctcga cgagctcgaa cccgtcgagc
    12541 ccgtagccgg gcatggcctc gtcgcgggtg acgcgtgtga gcccggcgcc cggctcgatg
    12601 ccgatgatct ggccgtcgaa catgtcggcc tggccggcga ggtcgccgat cgtctccatg
    12661 tcgacgtagg acggtacggc gatgttcagg gtgccctggt cgtaccaggt gccgaggtcc
    12721 tcgagctcgt cgccgtactc ctcccagtag tcctcgtggg tggagggcag ccaggcgtcg
    12781 aggaacacgt ccaggtcgcc ggcggcgagg ccctggaagg tgggggcggc gtcgagctgg
    12841 gtggtctcca cggtgtagcc ctcctcctcg aggatgtggg tccacaggtg cgtgaccgcg
    12901 atgtcctcgt cccacgggat catcccgatc tggacggtgc cgccagcggc ctcggcgtcg
    12961 tcgctctgct cgtcggtgga ctcgtcgccg gcggtggtgg tctcgtcatc gtcgtcgcca
    13021 caggcggcgg cgaacagcat gagggccagc agggccccga gcagtcgaag gttgcgttgc
    13081 attcgcattc tctctccttg gttgggtgaa ctcggtgaga tctgggtcag gcggtgcccg
    13141 ccgggggcgg gcggcgaccg gtcgagccga tcgaggcggt cgtgcgatcg aggaagacgg
    13201 cgaggatcac cacggcgagc ccgccctcga agccgacgcc gacgtcaagg cgggtgatgc
    13261 ctcggaacac ctcggcgccc aggccgccgg caccgacgat gccggcgatc acgaccatgg
    13321 acagggccag catgatcacc tggttgacgc cggccatgat cgtcggcagc gccagcggca
    13381 gctggacctt gcgcaggacc tgtcgcggtg gtgcgccgaa ggactcggcc gcctcgacca
    13441 cctcggtgtc gacctggcgg atgccgagct cggtgagccg gacgccgggc ggcatggcga
    13501 agacgaccgt ggcgacgacg cccgggacgg cgccgatgcc gaagaaggtg atcgccggga
    13561 tcaggtagac gaacgccggc atcgtctgca tgaagtcgag gaccgggcgc agcaccgagg
    13621 agacccgttc gctgctcgcc gcggcgatgc cgagcgggat gccgatggcc accgccacca
    13681 gcgtcgcgat gagcaccaga gacagcgtgg acatcgactc ctcccagagc gtcatgctct
    13741 cgatcaggag catcgacagg aacgtgaaga tgccgaaggc caggctccgg agccgccagg
    13801 ccacgagcgc gaagagcgcg gccagcacga tcgccggtgg ccacaggagc aggtcctcga
    13861 ggtggtccac gagccagcgg acctggtccg agacgaagtc gaagacgtcg ccgaggttgt
    13921 cggtcagcca gtcgacccct tcgtcgaccc ggtcgccgat ggggatgcgg gggatctcaa
    13981 ccatggtcgg cctccggtgg cgtgtcgacc gtctggccgc ccaaggcggc gagcagggtc
    14041 actcggggca ccaccccgac gagccggccg tgctcgtcct cgacgggcag cggcaggggg
    14101 gtcgccgcgg ccgggacgaa cagctcgacg agcgcgacgt ccggcgagat ggtggtcagg
    14161 tcggagcgca gggcgccatc caggcgctgg gctccgtcgc ggatggcagc tgcgacgtcc
    14221 tcgtcggtca cgtagccacg gagcctgcgc tgcgcgtcca ccaccagcag cgccgacacc
    14281 tggagcgatc gcatctcgcg catcgcggca cggggcccgt cgtgggtggt gatgaccgcg
    14341 tgggccttct ccatcacgga accggcggtg agcacccggg accggtcgac gtcctgcacg
    14401 aagctggcca cgtagtcgtt ggccggatcc tggaggatgt cctcggccgt gccgacctgg
    14461 accaggcgac cgtccttgag cacggcgatc cggtccccga ggcgcatggc ctcgttgagg
    14521 tcgtgggtga tgaacacgat ggtcttgtgc agggtctcct gcagctcgat gagctggtcc
    14581 tgcatctcgc gccggatgag cgggtcgagc gcgctgaacg cctcgtccat gaggagcacg
    14641 tcggcgtcgg tggccagcgc ccgggccagc ccgacccgct gctgcatgcc accggacagc
    14701 tggtccgggt agcggtcctc ccacccttcg aggcccacca tccgcagcgc ctcgcgcgat
    14761 cgctccgtcc gctcgctccg gtcacgccgg cggacctcga ggccccacgc agcgttctcg
    14821 agcaccgtgc ggtgcgggaa cagcgcgaag tgctggaaca ccatgctgat gctggcgctg
    14881 cgcacctccc ggagcgctgc cggcccgagg tcggtgatgt cgcggccgcc gagcttgatc
    14941 gtccccgccg tcggctcatg caggcggttg atggtgcgca cgagcgtcga cttccccgag
    15001 cccgacaggc ccatgacgac gaacagctcg ccctgctgga tgtcgaggct gatgtcgatc
    15061 accgcaggga cggcacccgt gcgctcctgc acctcggcgc ccggcacccc ctcgcgcagc
    15121 agctcgagtg cctcgtccgc gcggcggccg aacagcttgt agacgttctg gagttcgatc
    15181 gttgacatgt ggtctccggt ccggcgacac gactcggtgg cgcgacctcc aggagaacca
    15241 ggaccgcggt cgtggctggg aggcctcagc gagcggtgat cgaccagcga ccaacccgtc
    15301 gcacctcacc gcgtcagcgg cgggcgaaga ggcacatcca gttctcctga gtcggtcgct
    15361 tccgcccggc tcgcgttgtt cgagatcgag tgtggcagga accggcagcg aatccctgat
    15421 cggctcctcc cctcccggcg ttaccgtgcc cgcccgtggg tgcgatcgag gtgagcgatg
    15481 cggggtaccg cctgcccggc gggcgaccgc tgttcgacga cgtctcgttc cgggtcggcg
    15541 acgggcagaa ggtggccatg gtcggcgcca acggcgtcgg caagaccacc ctgctccgcc
    15601 tcatcgccgc caccgcgacc ggcgaacccc acgacgacca cgacgggacg atccgcgtcg
    15661 acgggcgggc cgccgtcatg gaccagctgg tgggcatgcg gggcacaggc ggcacggtcc
    15721 gggacctgtt cgtctccctc gcgccgcccc ccgtgcgggc cgcagcggag cagctgaccg
    15781 ccgccgagcg ggccatggcg ggagccgagc ggggcggcaa gccctcgttc gtggccgagg
    15841 ccggcatgcg cctcgccgag gcccacggcc ggtggggcga cgtcgacggc tacgacgccg
    15901 aggtcctgtg ggaggtgtgc tgcaccgagg ccgtcgggct ccccctcgac gtcgcctgcg
    15961 aacgcccgct ctccacgttc tccgggggcc agcagaagcg cctcgccctc gagtacctgc
    16021 tccgcagcga cgccgccgtc ctgctgctgg acgagccgga caacttcctc gacatccccg
    16081 ggaagcgctg gctcgagcag cggctcggcg agagccgcaa gaccgtgctg ttcatcagcc
    16141 acgaccggga gctgctcgca gccagcgccg acaagatcgt caccatcgag ggcgacggcg
    16201 cctggaccca cggcgggtcc ttcgccacgt accaccaggc ccgcgccgac cgccacgccc
    16261 gtctggccga cgaccaccgg cgctggaccg aggagcgcaa gcgcctcgtg gagctggtgc
    16321 gggtcatgaa ggagcgggcg aaggtctccg acgccaacgc atcccgggcc cgggccctcg
    16381 agacccggct gcgccacttc gacgaggccg gcccacccca ggagcagccc aaggagcagc
    16441 gcatcaccat gcgcatcgcc ggcggccgca ccggcaagcg ggcgatcacg atggaggacc
    16501 tcgagctcat cgacctgacg ttcccgttct cggcagaggt ccactacggg gagcgggtcg
    16561 gggtgctcgg ggcgaacggc accggcaagt cgcacctgct ccgcctgctc ggcggcgacc
    16621 gcaccgtcgc ccacgagggc cgctgcgagc tcggcgcgcg cgtggtgccc ggccacttct
    16681 cccagaccca cgaccacccc gagctgcacg gccgcaccct cgtcgacgtc ctgcgcgccc
    16741 gcgacctcga cctcggcgcg tcgatgagcc ggctgcggcg ctacgacctc cagggcgcgg
    16801 ccgaccagac cttcgagacc ctctccggtg gccagcaggc ccggttccag atcctgctgc
    16861 tggagctcgg cggcgccacg ctgctgctgc tcgacgagcc caccgacaac ctcgacctgg
    16921 tctcggccga ggcgctggag gacgcactcg aggccttcga cggcaccgtc gtcgccgtca
    16981 cccacgaccg ctggttcctc cgctcgctcg accgcttcct ggtcttccgc gaggacggcg
    17041 aggtcgaggt gtgcgacgag ttcagctggg ccaggactgg cgcagcttct cccacgtgac
    17101 cgacagcggc tcgggcagca cccgggcctc ggcgacggtg gtcatgaagt tcgagtcggc
    17161 gtcccagcgg ggcaggacgt ggccgtggac gtgggtcggc accccggcac ccgccgcccg
    17221 gccgaggttg aagccgacgt tcacgccctc gggccggtac gccgccttga tggcgcgcac
    17281 cgcctcgttc accaggcgcc acacgtcggc ggactcctcc cccgtcaggt cctcgagcgc
    17341 gccgacctcg cggtagggca tcaccagcag gtgcccggtg ccgtaggggt aggcgttgag
    17401 gatggcgaag gcgtgatcgc cccggtggac caggtaggcg gtctcgtcgg tgaggtcacc
    17461 ggcctcgatg gcacgcagga tgcgagcgaa caccgaaccc ccctcggtgg ggagcccagc
    17521 gttgccatcg gcggtgacct cgtccatgta cgccgtgcgc cacccggccc acagccggtc
    17581 gaggggcgag cgggctccct cgcgatcagg cgtggtcacg gatctcggcg gtgatgcgct
    17641 cgatgaggtc ggcgacgggg atgtcgcgct cgggcttgtc gccgccgcgg acgttgtcgg
    17701 cgacggtgtc gttggccacg tcgtcgccgc ccaccacgag gatgtggggg accttctcca
    17761 tcttgtggcg gcggatgcgg gcaccgaggc cgtcgtcggc cggggacacc tcgacccgga
    17821 cgccggcctc gcggaggcgg tcggcgacgg tctgggcgtg gtcctcgtgc tcgtcggcca
    17881 ccgggcacac cacggcctgc accggggcga gccaggtcgg gaacgccccg gcgtagtgct
    17941 cgagcagcac gccgaagaag cgctcgatcg agccgaacag ggcgcgatgc aacatgatcg
    18001 gtcggtggtg ctcgttgtcg gcgccgacgt aggtcagatc gaacttctcc gggtggttga
    18061 agtcggcctg gatggtcgac agctgccacg agcggccgat cgcgtcccgg acgtcgatgt
    18121 cgatcttggg cccgtagaag gcggcgtcgc cctccttgac gccgtactcc agcccgtggc
    18181 ggtccagcgc ctcgcgcagc gcctcggtcg ccttctccca gatctcgtcg ctcccgacgt
    18241 acttgtcggg gtccttggtc gacaggttga acgtgaagtc ctcgaagccg aacgcccgca
    18301 gcacggacag gacgaagtcg agcaggccgg cgatctcgtc ggcgagctgc tcctcggcca
    18361 cgtagatgtg gctgtcgtcc tgggtgaacc cccggatccg catgagcccg tgcagggtcc
    18421 cggcccgctc gtagcggtag acggtgccga gctcgtacag gcgcagcggc agctcccggt
    18481 agctgcgctg gcgtccccgg tacaccaggc agtgcatcgg gcagttcatc ggcttcatgt
    18541 agtacgtgcc gttgtccatc tccatcggcg ggtacatgcc gtcggcgtac cagtggaggt
    18601 ggcccgaggt ctcgaacacg ccggccttgg tgaggtgcgg ggtgtagacg aactcgtacc
    18661 cgccctcctc gtgacgggcc cgcgagtagt cctccatcag cttgcggacg atgccgccct
    18721 tggggtgcca gacggcgagg ccgccgccga tctcgctcgg gaacgagaac aggtccagct
    18781 cggtgccgag ccggcggtgg tcgcgcttgg cggcctcctc gaggcgctgg aggtgctcgg
    18841 ccagctcggc cttggtggac cacgccgtgc cgtagatgcg ctgcagctgg gggttcttct
    18901 cgtcgccgcg ccagtacgcg ccggcgacgc gcatgagctt gaagtggccg aggtggtcgc
    18961 tggtggtcgg cacgtggggg ccgcggcaca ggtcgatgaa gccgtcgtgg ccgtggaagg
    19021 tctggttcgg gcgcggctcg ggcgggatgt tctcgtagca gcgcacctca ccccgctccg
    19081 acgtggccga catcgggtcg gtggacgcgt tctcgatgat ctcgagcttg tacttgtggt
    19141 cagcgaagat ctggcgggcc tcgtcggcgc cgatctcgct cttcacgaag ggctgggact
    19201 ccttcaggat ctcccgcatg cgctcctcga tgcgctcgag gtcctcctcg tggaaggtgc
    19261 ccccgtcggg cagctcgaag tcgtagtaga agccgttctc cacgggcggg ccgatgccga
    19321 aggtggcgcc cgggaacagc tcgagcaccg cctgggccag gacgtgggcc gtcgagtggc
    19381 ggatgacgta gaggccctgg tccgagtcgg cggtgacgat ggcgaccgcg tcgccgtcgg
    19441 cgagctcgtg ggagaggtcc cgctcgatgc cgttcacctc ggcgatcacg gccttcttgg
    19501 cgaggcccct ggcgatggcg cgggcgaggt cgccggcagt ggacccggtg ggcagctcac
    19561 ggctggatcc atcggggagc gtgacggcga tctggtcggg catctgggcg aggctactga
    19621 cccctctgcg gacgccagaa cacggatagc agggcgaggg ccccgccgaa gccggcggcg
    19681 agcacgatcc acagcccgtc gctgccgggg acgggctgga acaggtcgag cgcctcgtcc
    19741 agcgaccagc cacccgggcc gaccacgccg agggccacgc cggcggcgac gagggtcatc
    19801 acgtactccc agccctcacc gggccggaag atgaagaagc cgttgccccg gtggttggtg
    19861 atcagcgcca cggtcatcgt gccgacgacg gcggcggcac cgagcggcgt gagcagcccg
    19921 agcaccagcg agatgccggc ggcgagctcg gccaggctgg ccagccaggc gtgcaggatg
    19981 cccggcttca tgccgagcga ggcgaaccac ccggctgtgc cctcgatctt cccgccccgg
    20041 aacacgtggt tccacccgtg ggcgaacatc acggcgccga cggcgcagcg gaagacgagg
    20101 agggcgaggt cgatggcgtc ctggtccgtc aagaccacca cttcgacagc acgtccgccg
    20161 acgaccgccg gacgtcgacg tcgtggtgga cctcgatgtc ctcggcgtgc tcgacgtcgt
    20221 gtgcgccacc ggcgccgacc agcacgcgct cgcgctgcgg tgcgggctcg ggctccggct
    20281 cggctctcgg atggccgttg ccgttgccga gctcggccgg ttcggcgacc ggtgccggcg
    20341 ccgcgacctc ggaggcctcg ggatcgcgct cgtcgtcgcg cttgttggcg tactcgtcga
    20401 cgtactcctg gcccgagagc ttgcggatct cgtacatgac ctcgtcgatc agctggcgca
    20461 gcacgaggcg gtcatcggcc cggtcgcggt agcgggtgac gtcgatgggt gcgccgatgt
    20521 tgacctggac ggaccggaag ggctggggca gcgaggcgcc cgccggttgg atgcggtcgg
    20581 tgccgcggaa gccgacgggg atcagcgggg ccccggtgcg cagggcgagg cgggcggggc
    20641 cggtgtggcc gcggtgcagc cgcccgtccc gggagcgggt gccctctggg tagatgccga
    20701 acagctcgcc gcgctcgagg acgcgggtgg cggtgtcgag cgcgcgctgg ctggcgctgc
    20761 ccccctcccg atcgatgggg atcatgccga tggccgggaa gatgtacttg gtcttccagg
    20821 agtccatgta ctcggccttg cccacgtagc tgatgcgtcg gggcagcacg cagggcagga
    20881 cgagggagtc gaagaacgag atgtggttgg ggcagaagat cgccgggccc tcggcgggga
    20941 cgttgtgcgc accggtgacg ttgatgcgcc acagggcgtg catcaacggt cggatcaggc
    21001 cctttgcgac cggatacatc tttccgtact cgcgctcgtc ccccacgggt cggaacgtac
    21061 cagcgggaac gctccgtgcg tcggcagttc gctacgaacc agggaccagc tggacgccga
    21121 gcagcagcgc agcgtcggtg acgtcggcgc cggccgccgc cgcgacgtcg atcgcctcga
    21181 cggctccggg cgtgtcgagg tcctcgtcga ggtgggcccg gaccgtctcg agcacggcac
    21241 caccctcgcc gccgtcacca gcacgccagc gggccaggcg ctcggcggcc tcgggcatga
    21301 ggtcctcgtg ccactcccag ccgtcccggt agtggtgctt gacgaccgcc aggcggatgg
    21361 ccatggcatc ccactccttg cgcagctcgg acacgaagac caggttgccg agcgacttgg
    21421 acatcttctc gccgtccatg cggaccatcg cctggtgcat ccagtggcgc acgaagggct
    21481 cgccggtggc cgcctcggac tgggcggcct cgcactcgtg gtgcgggaag atgaggtcgg
    21541 cgcccccgcc gtgcaggtcg atggtggtgc cgagctcgcg cagcgccagg gccgagcact
    21601 cgatgtgcca gccgggccgc cccggacccc acagcgagtc ccacgacggc tcgtcgggca
    21661 gggacggctg ccagagcacg aagtcgagcg cgtcccgctt gttcgggtcg tccacgttcc
    21721 cgccccgctc ggcggcgtac tcgagcatcg tgtcgcggtc gtagcccgac acctgcccga
    21781 acccctcgta ggtcgagatg tcgaagtaga cggcgccgcc ggcctggtag gcgtggcccc
    21841 ggtcgagcac catcccgatg aagccacgga tgtcggcgat ggccgacgtc gcccgcggct
    21901 cgctccagga cggcagcatc ccgagggcga ccatgtcgtc gtcgaacttg gcggtctctg
    21961 cggcggcgag gtcgaggtag tgcacgccga tctcgcgcgc ccggccgagg atggagtcgt
    22021 cgacgtcggt gacgttgcgc acgcagcggg tctggtggcc ccggtcccgc aggcgacgct
    22081 ggagcacgtc gtagcccatg tacgccgcgg cgtgtcccag gtgcgtggcg tcgtacggcg
    22141 tgatgccgca cgtgtacatc gtgacgatct cgcccggctc gaacgggacg atctcgcggc
    22201 gcgccgtgtc gtagagccgc agcgggctca cttcgagttg gggtccccgg cgtcgcgctc
    22261 cgcagccacg gtggcgcggg gcggcttctt cggcaggccg gtgaggctgt cgggcaagcc
    22321 gttgaacttc accgcgacgc gcgtcttgta gcgcacgttg agctgcagca gcaccgccgt
    22381 gaacgactcg atcggcgtcg actgggacag ctcgccggcg tcgagggggc gcatgttcgg
    22441 gatggcctgg atcagctcgg tggtctgggc cgtggcctcc tcgtggtccg agcagatcag
    22501 cacgtcggtc tcgatcgggt ggtcgaggtc gcccagctcc ttggccggca cgtggtggaa
    22561 cgcgcccgcc accttgcagt cgggcaccac ggcctggatg ctggcggcca cgctgccccg
    22621 cggcggcacg agcggctgga actccttgcc gatgcgggtg agcgcgttgg ccatggagat
    22681 gacgaccttg ccgctcaggt ggtcgctgac cgaggccgcg gtctgggtgg cgccgtccca
    22741 cggggtggcg acgaccacga cgtcggcagc ggctgctgcg gcgttgtcgc cggcctcgat
    22801 ggccagggtg cggcccggcc acttgtcgag cagctcctcg accttctcct tggcccgctc
    22861 cgcggaccga gagccgatga tcacgtcgta gcccacgtcg gcgaggcgtg ccgcgagtgc
    22921 ggagccggcg ggacctgttc ctccaaggat tccgatcttc acggcgcgga ctctaccggt
    22981 ggggtcctcg gcggaagcca aaccggtccc aacccggtcc taacctgcga gccgtgccgg
    23041 accccaccgc cgaccccgcc gcgggcccgc cccggcagct ctcccctgtc cccttcgtgg
    23101 tcgccctcgt cggcttcgtc gtcatcgccg cgctggcctt cgtgttcctc cgcgccgacg
    23161 acgagggccg cctggtgcgg cctgaccggt tccaggtcct cgacgccgac acgatcaccg
    23221 ccaccgcgct cgggcaaccg gggtgcgggc gggtcgagcg cgcccaggtc gacctggccg
    23281 acaccgaggt cctcgtcgag ctcgtcgtcg tggacgtcga gggaccgtgc tcggacgtga
    23341 cggtggacct cgtcgtcgag atcacgctgc ccgaaccggt cggcgaccgc gccgtgcgcg
    23401 ccggggtcgg ccgcacccgg ctgccctgca ccggcgacgc gagatcgacc acgtgcgggc
    23461 cgaccggctg agacgacccc ggtaacgtcg ccgcaccccc cgaggtcagg acaccaatgg
    23521 gaatcctcga cggcaagcga ctgctcatca ccggcgtcct cacggatgcc tccctcgcgt
    23581 tcggcgtggc caagctggcc caggaggagg gagccgagat cgtcctcacc ggggcgggtc
    23641 gagcgctgcg cctcacgcag cgcacggcgc gcaagctccc ggtcgaagcc gacgtgctcg
    23701 agctcgacgt caccgtcccc gagcacgtgc atgccgtccg cgacgcgctc ggcgcgaagt
    23761 gggaccgggt cgacggtgcc ctccacgcca tcgggttcgc ccccgagtcc tgcctcggcg
    23821 gcgggttcac cgaggcgccg tgggaggacg tcgccgtcgc catgcagatc tcggcgtact
    23881 cgctcaagac catcgccgac gtcgtcgtgc ccctgatgcc ggagggcggg tccatcgtcg
    23941 ggctcgactt cgacgccacc caggcctggc cggcctacga ctggatgggc gtggccaagg
    24001 ccgcgctcga gtccacgtcc cgctacctcg cccgcgacct cgggccgaag aacgtccgcg
    24061 tcaacctggt cgccgccggc ccgatccgga ccatggccgc caagtcgatc cccggcttct
    24121 cggccttcga ggacgtgtgg gacgaccgcg ccccgctggg ctgggacgtc aacgactccg
    24181 ccggcgtggc ccgcaccacc gtggcgatgc tgtcggattg gttcccccag accaccggcg
    24241 agatcgtcca cgtcgacggc ggctaccacg ccatcggggc ctaggcgaac cagcgggggt
    24301 tggcgacccg gagcttgtcg acgacggact ccgccatggc ccgcaccacc tcgtcgcccc
    24361 ggaggctgcc gtcgcggacc ccggcggcga acgcgagatc gtcggccacg ccgagtgatt
    24421 gcaggcgctc ggcgtggtcg gcctccatcg ccggccccat cgtggcctcc cgctccagcg
    24481 tggccagcac gttctccgcg acgcggctgt ggaacccgac tcggcccgat gcctgctcgc
    24541 gcacgtcgcc ggtcacccac tcccgcaccg cctcgagcag ctcggccgcc gtgggacgcc
    24601 cgtgcaggcc gggcgcgcct ccaccggtcg gtgccggtcg agcgacggcc gcggccagcg
    24661 cgtcgggctc gagcagcagg agcaggtcgt gctcgttctc gcacacccgg cgcccgatgg
    24721 cggccagctc catggagcgg gacaggccct ccaggtgggc ccgggcctgc atgatgcaca
    24781 tgatccccca cttgagcgtg ccgaggacct cccaccacag gacgacgtcg cggtcgacgg
    24841 gacggcccga cgcctcctcg tacgcgtcga agagctggtc gtagggaccc acgcccgcca
    24901 ccggggcttc gctgccgaag cgccaggccc gggtgcacag ccagccgagg tcctccatcg
    24961 ggtcgccgat gtgggccagc tcccagtcga tgaccgcctc caacccatcg ggaccgacca
    25021 tgaggttgcc gagccggaag tccccgtgga cgatcgcgtc accgctcgac gggggccggt
    25081 tggcctccag ccaccggaac gcgtgctcga aggtggggtg cgggtcgccg aagtcgtcga
    25141 ggaccgtgcg gtactgggcg acctggtcgg tgcgctccag gccgggcacg gcgtcggtgg
    25201 ggacccggtg gatcgcggcc agcgcccggc cgcagtcggc ggcgaggcgg tcccgcgccg
    25261 tcgcgaactc gtcgtcgcgc aggatcttgc gggcgatcgt ctccccgtcg acgaagcggg
    25321 acacgaggta cccggcgccg aggtcacccc ggtcaccggg tccaccagag gcgctgacga
    25381 gggatggcac gggcacgccg gcgtcggctg cggcccgcag catcgacgcc tcggtggcca
    25441 tcgcaccggt gcggggcgac ccggtgcgct cccgctgcag gatgaaccgg tcaccgccgg
    25501 cctcgaaggc ccacgtctct cgggacgcac cgccggtgag ccggcggagg ccgctgacct
    25561 cgaggtccgg gtcggcgagc tcggttcgga gcaggtcctg cagtcggtcg gcgagctcgt
    25621 cgggcgtcac gccggggatc ctgccagatg cggcaacacc cgttgccaac cagccgccag
    25681 gccggcgagc tagccgcagg cggcgcgggt ggcggggtac gggttgacgg ggccgctgtg
    25741 gttggggtgg atctcgaagt gcaggtgcgg catgccccgg gcgttgccgg tgtcaccgac
    25801 gtagccgatg accgtgccgg cggagacccg cccggacgcg ccgtaggccg agaggtgggt
    25861 gccgtagtag tagtgcccgt tgtcgccgga caggtggaac gacagaccgc cgatggagtt
    25921 gccgcggtgg ctcaccgtgc ccgacaccgg ggcgacgacc gggacgccgg tgcgggccat
    25981 catgtcgacg cccttgtgcg agcggccgcc ggagcggggg tagccccagg agtcgatgaa
    26041 ggagtgcggg ccggccaccg ggcagaccat gccgccgccg ctcgacgtgg ccggggcggc
    26101 ggccggggcc ggggccgggg ccgggctggc cgcacggctg gcggaccccg aggtggtggc
    26161 gctgctgcga gcggcctgcg aggtgcgctg ctgggcctgg gcttcggccg cggcgcggcg
    26221 ctgggcttcg gcctcggcgg ccttgcgccg ggcctcctcc tcggcgcggc gcttggcctc
    26281 gagctcctcg aggcgcttca gctcggcttg gacgtcggcg agcgcccggc gcagcgactc
    26341 gatgctggac tcctgctgct ggcgcttcgc ggcgagggcc gcactggcct cgtcgagctc
    26401 ggccttggcc tcggtgaagt cgtcgatggt gtcgaaggtg ccctggctga cgtagcgggc
    26461 gaacgcctcg gccttcacgc cctcgttgat gtcgtcggtg ccgagcagcg gcatgtcgcc
    26521 gctggacgtg taccgctcga ccacgagctc ctccatcgac cccgacaggg cggcgaggcg
    26581 ctcctcggcc tgggtggcct tggcctcgag cgaggcgatg tcgcgctcga tggcgccgag
    26641 cgcggactcg gcgtcggaga cccggcgcac ggcctcctcg gcggcggccc gggcgcgctg
    26701 gatgtccgcg gtctgggcgc cggcggccga gggcacggcg agggatgcgg cgacggcgag
    26761 ggtcaccacg aggggggcga ggaggcgatg gagcatggtg gggacagccg gtgggccgac
    26821 gggccaggct acgacccggt tgcagatgtt gcaatccggt gacggacctc aggccgacgg
    26881 caggggccgg gtccgggtgc tgcggacggt ccgcggcttg tcgctctcgt cgacgatctc
    26941 gcccaccagc tcctcgagcg cgtcctcgag gctgaccaga ccgacggtgc gcccgtgggc
    27001 atcggccacg atggcgaggt gcacccgggc cttctgcagg gcgtggagga cgtcgtcgac
    27061 gatggtgtcg tcgcgcacga tgggcagccg gcggatcttg ctcagcggga gcggggcgcc
    27121 gcgcagggcc tcggggaccg tgagcaggtc cttggcgtgc aggaagccca gcacgtcgtc
    27181 gaggcccccc tcccccacca cgagcaaccg ggtgtggccg ctgcgcacca cctcgcgctc
    27241 ggcctcgacc gtcgtggcct gtcggggcac gtacccgatg cggtcgcgcg ggacgaccat
    27301 cggcccgatg ggggcctcgc cgatggcgag cgcgccgcgc aggatctggt cctgcacgtc
    27361 gtggatcagg ccctccttgc gggaggccga gaccatgtcg gcgagctcgt cggaggtgtg
    27421 gctgtcggag atctcgtcgg caggctcggc gccgaggagg cggatgatgc cgttggcgag
    27481 ggcgttgagc accacgatga ccggcccgaa gatccgcatg tagagccggt tggcccagtc
    27541 gagcacgagc accgtgcgct ccggctcggc gatggcgatg ttcttgggca ccatctcacc
    27601 caggaccatg tggaggaaca ccacgatgga cagggccaga ccgaagccga ccgtggtggc
    27661 cacgccctcg gacacgtcga tgcggtcctg cagcaggtcc tcgagggcgt gggcgatggc
    27721 gggttcggcc acgtagccga gcagcagcga gcagatcgtg atgccgagct gggcgccggc
    27781 gagctggcgg gacaggtcct ggatggagtc catcgcccgc gccgcggcct tgctgccctc
    27841 gtcggccagg gcggcgagcc tcgtgcgccg gctggccacg agggcgaact cgaccgccac
    27901 gaagaacgcg ttcgcggcca gcagcagcac gctggcgatg agggcggtgg cgttcatccg
    27961 ccgctccgct cgaccaggac gacctcggcg atgcgcatgc ggtccatctc gcggacggtg
    28021 accgtccagt cgccgtggtc gaaggcatca ccctcgacgg gcaggcggcc gagggtgtcg
    28081 agcacgaagc cggcgagggt ctcgaagggg ccgtcgggca gctcgaggcc gcaggcgtcg
    28141 aagacctcgt cgcggtgcag gccaccgccg agcacccact cccccgggcg ctgcacgcgc
    28201 gtcagggccg gcgccgcccg gtcgtgctcg tcgtcgatct cgccgacgat ctcctcgagc
    28261 acgtcctcga gggtgatgac gcccgcggtg ccgccgtgct cgtcgaccac caccgcgagg
    28321 tgcgtggcct gctcgcgcag cagcacgagg agcaccgaca gctgcatcgt ctcgggcacc
    28381 accaggggct gggccatgac ggagcggacc gaggtggtgc cccgctgctc gtaggggacg
    28441 ccgaacgcgt ccttcacgtg gctgacgccg acgatgtcgt cgaggtcgcc gtcgtagacc
    28501 gggaagcggc tgaaaccggt ggtgacggcg cgcgcggtca ggtcggcgac ggtctcgtcc
    28561 acggcgacgc cgacggtctc cagccggggg acgagcgcgt cgacggcgga cttctccgag
    28621 aaccgcacgg cccgggtgag caggttcacc gagtcctcgt cgatcgtgcc ctcctcaccc
    28681 gaggagcgga acagcaggtc cagctcctcc aggccccgca cggaccgcag ctcctcctgc
    28741 ggctcgatgc cgaggcggtg caccacccag ttggcgcacc cgttgaacag ggcgaccacc
    28801 ggcttgacca cgatgccgta gacccgcatg acgggggcga ggaagtaggc ggtgccggtg
    28861 gggtacgtga tggcgagcga cttcgggacg agctcgccca gcaccatctg cacggtggtg
    28921 gccagggcca gcgcgacggc gatggacacg ccccgcccgc tgctctcgcc gaccacggcg
    28981 gcgacggccg gctcgatgac ggcggcgacg gtgggctcgg cgatgaagcc gagcacgagc
    29041 gacgtgatcg tgatgccgag ctgggcgccg gacaggtggt acgagagccg gcggagcagc
    29101 ttctcggtga cccgggccgc acccttgccc tcccgggcgt ggcggctgac ccgccctcga
    29161 tcgacggcca cgagcgcgaa ctcgttcgcc acgaacgcgg cgttggcggt gatgaggacg
    29221 gcgacagcga ccaggccgag tgcggtttcg atggggcaca gggtacaaag gcccggtgtc
    29281 caccgagcca cccgacgcga cgcctgcgga ggcccccgac gccgtggtcg agctggtcgg
    29341 cgtggacgtc gccgtcgacg gcgtcccggt gctcaccggg ttggactgga ccatccgagc
    29401 cggcgagcgg tgggtgatcg tgggaccgaa cgggtcgggc aagacgaccc tgctgcgcct
    29461 gctctcgtcg tggctccggc cgtggtcggg gcgagcggtc gtgctcggcg agacgctcgg
    29521 tcgcaccgac atgcgggccc tgcgccggcg ggtgggctac acgagccagg cgctcaccga
    29581 cctgctccgt cccacgatga cgccgcacga ggtcgtgctg gcggcaccga acggcgccct
    29641 ggagacgtgg tggcaccgct acggcgacga ccagcacacc gatgcctcca cccagctgcg
    29701 gctcttcggc atcgacgagg agaccgagca ccgcccgttg cgcaccctgt cctcgggcga
    29761 gcgccagcgg gtgctgctgg cccgggcgtt caccggccgg cccggcatcg tcatcctcga
    29821 cgagccgacg gcgggtctgg acgtcggcgg tcgcgaggac ctgctccggc gcctcgacgc
    29881 ggcagctgcg acgaccaccg cgccgctcgt gctggtgacc caccacctcg aggagatccc
    29941 cgccgccttc gaccgcgtcg tgctcctcgc cgacgggcgc atcgccgggc agggcccggc
    30001 cacgtcgctg ctcaccagcg cgcggctgag cgacctctac ggcgtggacc tcgacgtggc
    30061 ctgccgcgac ggtcgctggt cggcggtcgc agccacccgc accgccgact gacgacggct
    30121 caggactcga cgagggcgcg gagccgctgg cgcgacgcct cgatctcgct ggtcagcacc
    30181 cggtcgagca gcggcaccca gagcgacccg ccgtagtgga ggtccatgag caggcaggcg
    30241 cctgtgggcg tgggctccac cgtcgcggtg agccgccacg cggcgtgctc gcgcccgtcg
    30301 gtctcgcgcc gttcgaacac ggcccggccg ggttcgcctc cgaccggcct cgtgtgctcc
    30361 gtgcgcacca tgcgcaggcg cttggaccgg gcgagcggcc cgagcctccc ccgcagggtg
    30421 acgtaccagg ctggacccgg gtcgccgtcg atggcctcgg cgtcggcggt gcggggcacg
    30481 atctcgagcc agcggtcgta ggtgtcgagg ccaccgaccc actcgaagag cgtgtcgggg
    30541 gtgcagtcgg cctcgaggct ggcgcgcagg tccacggcgt gcaggtctac cggcgcgaca
    30601 ggtcggcgac cacgcgcagc aggccgtagg ggttcacggc gtcgccgccg ttgggatgga
    30661 cctggaagtg caggtgcggc gcgccgccct gggcgttgcc cgtgtcgccg acgaagccca
    30721 ccacgtcgcc ggcgttgacc agcgcgccct cgaccatgcc ctcggcgaag gcagccaggt
    30781 gggcgtagta gtagtacgtc ccggactggc ccttcagcca cagcttggtg ccgccgagga
    30841 cgtcggtgcc gacccgggtg atgatgccgc gctccaccgc gtagagcggg gtgtcgaagg
    30901 gcgccatgat gtcgacgccc tggtggccgt gctcgtactc ggtgccgacc atgcgggggt
    30961 agccccacga gtcgatgaag tcgtagggct ccccgaccgg gaacacgaag ccccggatca
    31021 cgatctcgga gccggcgctg aacaccgcca gctggaactt gcgctcggtg ttgatgcgga
    31081 gcgagtcctg cagggcctgg cgggcgctgc gcagctcgcg ccgggtctgg gccacggcgt
    31141 cgacggcgtc caggacgccc tggtcgatga cggccttggc ctcctgcagg tcggccaccg
    31201 actggctgtc ggcttcggac acggaggcga ggaagacctt gcgcatctcg agcacggtgc
    31261 cgtcctggga ggtcatgacc gtgtccagct cggccgcgtt gccgcgcacc accgcgttgg
    31321 cgatccgctc cttgaaccgg acctgggcct cctcgagctc ctggaccttg agctgctggg
    31381 ccacgtcgag gtgcgccagc tgctgctgca ggtcggccag gcgatccgac aggtcgtcga
    31441 ccagggtcgc cgcaccttcg acctcggcgg cgctctggag cacctcggcc tgggcgacgc
    31501 gcagctcgcg acggatgagg cgctcggcgg cgagcgggtc cacgggctca ccgggcggcg
    31561 gcgggatcgt ctcctccgac agcggcaccg actcgggggg cgagtcctgg tcgcccgcct
    31621 ggtccggggg tgttgtcggc gccgtctcct ccggaggcgg cggctgctcc tcgccgccgg
    31681 gcggcggcgg gggcgtcgtg gtggtggagg aatccgccgg cggtggcggg ggcggaggtg
    31741 gcgccgtcgt ggtggtcggg cacggctccg gcgtgccggg cgtcgtcgac gtcgtcgagg
    31801 tcgaggtcgt cgagcagtcg accggttccg tgggcggcgg atcctcctgg gccagcgccg
    31861 gcgcaccgac ggcgaccgag aacacggcga gcgcggtggc gaggacgcgg aggggggtgg
    31921 acggggtgcg tgtcatcgac gggggtgctc gttccagggc ccgccctgga tctctcgttc
    31981 gttcgcgcat cgtagggcgc cggatagcgt cgcggcccat ggagcgcacc ctgttcgacg
    32041 acgagcacga gctgttccgg gagagcttcc ggcgcttcgt cgccgacgag atcaccccga
    32101 acctggagca gtgggaggcc gacggcatcg ccgaccgctc gctgttctcg aaggccgggt
    32161 cccacggctt cctcggcatg gccgtgcccg aggagcacgg cggcggcggg gtcgacgact
    32221 tccgctacaa ccaggtggtc atcgaggagg tgcaggctgc cggcgccggc ggcgccgggc
    32281 tgggcatcac gctgcacaac gacatctgcc tgccgtactt cctcaccctg acgaacgacg
    32341 agcagaaggc ccgctggctg ccgggcatcg cctcgggcga gctcatcacg gccatcgcca
    32401 tgaccgagcc cgccatcggc tcggacctgg cctcgatgac cacgagcgcc gtgcgcgacg
    32461 gcgacgactg gatcgtcaac ggctccaaga ccttcatcac caacggcatc aacgccgacc
    32521 tgatcatcgt cgcggtgaag accgacccct cccagaagca cgccgggatg tcgctgctcg
    32581 tcgtggagcg gggcatggac ggcttcgagc gcggccgcaa cctcgacaag atcggcatgc
    32641 acagccagga caccgccgag ctgttcttca acgacgtgcg ggtgcccgcc gccaacctca
    32701 tcggcgacga gggcaagggc ttcctgcacc tggtcgacaa gctccctcag gagcggctgt
    32761 cgatcgccgt cgccggcgtc gccgccgcca aggccggcct cgactggacc ctcgactacg
    32821 tcaaggagcg caccgcgttc gggcagccga tcgggaagtt ccagaacacc cggttcacgc
    32881 tggccgaggt cgccaccgag gtcgagatcg gccaggccta catcgacaag tgcgtgctcg
    32941 ccctcaacga ggggaccctc accgccgagg aggcctcgat ggccaagtgg tggtgcaccg
    33001 agctgcagca ccgggccctg cacgagtgcc tccagctctt cggcggctac ggcttcatga
    33061 ccgagtaccc catcgcccgc gcctacgccg acgcccgggt caccacgatc tacggcggca
    33121 ccaccgagat catgaaggag atcatctcca agcggcatct cggcttgtag ccggggtcag
    33181 tgctgcagct cgcggcgcca gggcgggtca gccccgatgc gaccacaggc gatgccggcg
    33241 gcctcgaccg cgaacgtgag cgcggtgtgc caccagtgct cgtcgaggtc ggcgagcgcc
    33301 cggcggtcgg tgatgccgtg ggcggcgaag gcggcgagca tcgcgcccac gaaggtgtcg
    33361 ccggcgccga cggtgtcgac cacgtcgacc ggcgcggcgg gcacgacctc gatcacaccg
    33421 gcgctgcaga ccaccgcggc gccctcggca ccccgggtga tcacgatcgc cgccggcaca
    33481 tcaccgtcat cgtctcgcag ccagcgttgg gcgaggtcga gcgggttggc gccgggctcg
    33541 atccagtcga tgtcggccac gctgaccttg accacgtcgg ccagcccgag ccaccggtcg
    33601 agccgggcca ggtagcgctc ccgcccgccg gcgacggccg gccgggggtt cggatcgagg
    33661 tgcacgaggg tgcggtggcg ggcggcggcc agcagtgcct cgagccgcga cgccgccggc
    33721 tccagcacca gggccatcga gccgctcacg tggaggaccc cggtgtcggc gggcaggtgc
    33781 aggtcgatcg tgcgcgccga caccgtggtg gtgcccgcga cgtggaaggc gaaggagggt
    33841 tcgaggtcgt cgggaccctg ggtgaccacg gcgagggtcg tgggttcggt ggtgatcggg
    33901 caggtgcgcc aggacacgtg ggacgcgtcg aggtggtcgc gcagcagctg gccgaaccgg
    33961 tcgtcggaga gcaccccgag gaagctggtc ggcgcgccga ggcgaccggc ggccaccgcc
    34021 gcgttgaagg gtgagccgcc cggcgcggcg tgccaggtgc ggccggaggc gtcggcgccg
    34081 atgaggtcga cgagggcttc gccgcagcac acgacacgtg gggtggtcac gcgctcaggg
    34141 tgccagactg gaattgactg gtcggtcaag tagcagccag cgttgttgac acactgtcaa
    34201 caacgagaag gaatgctgtg aagaacacga cttcgacgcg ggggtggctc cgggcgctcg
    34261 cgctggtgct gggcctcgcg atggtcgctg ccgcctgcgg cggcggcgac gagacctcca
    34321 ccgacaccgg cgatgccacg accggcaccg gcggcgtgga ggactcgatc cccgacgcac
    34381 ccgagggtga ccccaccccg ggtggggagc tgacctacgc cctcgaggcc gagaccaacg
    34441 gcggcttctg cctcgccgag gcccagctcg ccatctccgg catgcaggtc gccgccacga
    34501 tctacgacac cctcaccaag cccgatgcgg acggccagat ccagccgcac ctggccgagt
    34561 cggtcgagcc caacgaggac ttcacctcct ggaccatcac cctccccgag ggcgtcacgt
    34621 tccacgacgg cacccccctc gacgcccagg tcgtggccaa caacatcgac gcctaccgcg
    34681 gcgcgtacga gaaccgcagc ccgctgctgt tccgcttcgt gttccaggac gtcgcctcgg
    34741 tccaggtcgt ggacccccag accgtccagg tcgacatgac ccggccgtgg tccgccttcg
    34801 actgggcgct ctacggctcg aaccggatcg gcatcatggg ccaggcccag ctcgactccg
    34861 aggactgcgg caccgagctc gtcggcaccg gcccgttcca gctcgagagc tggaccgtcg
    34921 gcgaggagct cgtcgccacg aagaacccga gctactggcg cacggatgcc gacggcaacc
    34981 agctgcccta cctcgacacc atcacctacc agccgatccc cgaggtcgcg agccgggtga
    35041 acgcgctcga ggccggtcag gtcgacgtgc tccacacctc cgacaccgag cagatcgccc
    35101 agcggctgca gccgctggcc gagcaggacc agatccagct gctcgcgacg gactcgtacg
    35161 gcgaggtcaa ctacctcatg ctgaacacgg acgcggcgcc cttcgacaac aaggacgccc
    35221 ggctggccct ggcccacgcc ctcgaccggc agctgctgat cgacgtccgg ggctccggcc
    35281 tcggctccgt cgccgacggc cccttccccg aggacgtccc cggccacgtc gccgaccccg
    35341 gcttcccgca gtacgacccg gccaaggccc aggagcacgc tgcggcctac gaggaggcga
    35401 ccggcgagcc gctgtcgttc tcctacacct tcgtctcgac cgagtccggg cagctgaccg
    35461 cccaggaggt ccagacccag ctggccgatg ccggcatcga gatcagcctc tcccccgccg
    35521 gtgaccaggc gaccaccatc aacaaggccc tcggcggcga cttccaggcc atggccttcc
    35581 gcaaccaccc cggcaccgac ccggaccggg agaacaactg gtggtacacg ggctcgcccg
    35641 tgaacttcgg ccgcatcgcg gatcccgagg tcgaccgcct cctcgacgag ggtcgcgaga
    35701 ccgcccccgg cgaggagcgc gacgccatct tccaggacct gacccggcgc ttcgccgagg
    35761 aggtctacaa cgtgtggctg tcgaccgcgg tgtgggcgat cgccacccag cccgacgtgc
    35821 acaacgtgct cggatacggc cccgaggccg gcagcgaggc cttccccggc gtcgccacgg
    35881 gccacgacgt cgccggcatc tgggtgagcc ggtgatcggt ggcgggcggc tgctcccccg
    35941 gccgcccgcc accccatctc cctggggcgc ccgccccgtc gctcgctagc gtcgcctggt
    36001 gctccccccg ccccgcctga cccggctcgt ccggtgcgtg ctcggactgg tgctgctcgg
    36061 cgtgggcctc gggctggtcg tcgcagccga tctcggtgcc gccccgtggg acgtgctgca
    36121 ccagggcctg tcccagcggc tgggcatcac gatcggcacc gccagcatcg gggtcggcat
    36181 cgtgatcctc ctggcctgga tcccgctgcg ggagaagccg ggcgttggca cggtgctcaa
    36241 cgtgttcgtg atcggcgcca ccatggacct cacgctggcg gtcgtcgacc gcccggacgc
    36301 gctgtgggtc caggtggcgt tcctggtcct cggcgtgtgg ctgtggggtc ccggctccgg
    36361 cctctacatc ggctccggcc tcggctcggg tccccgcgac ggggtcatga ccggcctcgc
    36421 ccgccggggc ctgcgcgtgg gacgggtccg ctcggccatc gaggtcgtcg ccctcagcgt
    36481 cggcttcctg ctcggcggca ccgtcggcat cggcacgctg gtgttcgcca tcggcgtcgg
    36541 ccccaacgtg gcgtggtggc tgccccgcct cgacatgcga gcggctaggc cagcagcgct
    36601 cgcgcgatgt gggtgatctg gatctcgtcg gtgccggcgt agatctgcag gaccttggcg
    36661 tcgcgagcca gctgctcgac ctggtactcg gacatgtagc cgttgccgcc gaagatctgg
    36721 accgcctcga gggcgacctc ggtggcggcc cgggccgagt acagcttcat cgccgacgcc
    36781 tccgcgagcg tcatggccat ctcctcaccc cgggcggcgg cggcctgcga ctcgatggtc
    36841 cggaacacca ggttctggac gttgaggcga gccacctcca tgcgggccag cttctcctgg
    36901 atcagctgga actgcccgat gggctggccg aactggaccc ggtccttggc gtactcgacc
    36961 gacagctcga ggcacttctc gatcatgccg agggccatcg cggccacgcc ggcgcgctcc
    37021 atgaggaacg tggccttcgc gccctcgcgg ccgcccttgg gcatgtcctc ggtctcgccg
    37081 atgaggcggt cgaggccgac ccgcacgtcg tcgaggaaca gctcgcccgt ggggctggag
    37141 tgcatgccca tcttgcgcag cggcttggac tgctcgaggc cgggcatgcc cgagtcgagg
    37201 acgaaggaca ggaccttgcg ctcctcgggc gggttgccct cgtccagctt gcagatgaag
    37261 acgatggtgt cggcgtaggg gccgttggtg atgaaggtct tggagccgtt gaggacgtac
    37321 tcgtcgccgt cgcggcgggc ggtggccttc atcgacccga aggcgtcgga gcccgagccc
    37381 ggctcggtga tggcccaggc gccgatcttg tccagcgtca gcaggtcgag cgcccagcgc
    37441 tccttctggc ggatggtgcc cttggaggtg atggccgatg cggtgaggcc ggtggagacg
    37501 cccatggcgg tgaccaggcc cgggctgtag cggcacagct cgatgatcgg gatcatcgtg
    37561 atcgagccgt cacccccacc accgccgccc tctcccccgg ccttctcctt ggtcgggggc
    37621 tcctcgccct tggcgatggc ctcctcgacc gacttctcga actcgatctg gcgcttgaac
    37681 cgctccctcg ccatcgtgtc catgccgaac gtgctgtaga gcttgcgcag gacgtcgtag
    37741 ggcggggtgt cgccgaactc gagctcgtcg cgccggggtg cgacctcggc ctccacgaac
    37801 tggcggacgg cgtctcgcac catctgctgc tcttcggtcc actggatcat gtcggggagg
    37861 tctcctcggt ggtacgtgca ggatcggtcg ggtcggtcag cagcgcctcg agggcggcca
    37921 cccgctcctc cagttcggca acgcgggcgt cgagccctgc cggtgccggg accggaaccg
    37981 gcaccggggc ctcgctcgtg tgctcgacgg cgcgctcgtc gggtccgtcg ccgagcaggt
    38041 gggcccagcg ggtctccttc tgcccgggct ggcggggcag gtggaccacg acgggatcgg
    38101 tgcgggccgc gagcccctcg agggtggcgg cgacctcgtc gttcgagtcg aacgcgtggg
    38161 cccgctcggt ccgggtccgc agctcgttca gcgtctgcgg gccgcgcagc agcaaggcgg
    38221 tgaccagcgc gagctcgccg gcgtcgaggc cgaggcgctc gtcgaggacg tggcggaact
    38281 tggtgctccg ggcgccgtgg ctggcgtaga cgaagcgcac caggtggcgg gccttcagct
    38341 cgtccagcgc tgcctggatc tccccctcgg tgtgctcggt gaccggctcg cggttgttct
    38401 tctggttgca ggcggtctgc aggccccgca gggtcatcgg gtacgtgtcg ggcaccgtgg
    38461 cggccttctc ggccagggca ccgagcaccc gctgctggat cggggtgagc ggaccctcga
    38521 gcgcctcggc catggggcga ccctaggccg acgaccctgt gaaccctgtg gttcactggt
    38581 gggcggggac gatgggcgga ggtcagacat gacggaggag accgagcagg tcatcgatct
    38641 cgcacccgcg ggtgcgtcca tcggcaccga cgcaccacgc agccgggcgg tgctcgtggc
    38701 gctggccgtg gtcgccgccc tcgccgcggc cgtgctcgtg ctggtgccgg gcgacgacgc
    38761 cgaggtggcc gtcgacgagg acggcgagac cgagaccgag accgaaccgg agccagacgg
    38821 ccgcgacgcc accgccttcc cggcgccgct ggcactcggt gcacccgacg acggcaaggc
    38881 atccgtcggc ctgcccgtga aggccgagcc cgccaccggc ctggtcgacg ggcagacggt
    38941 ccaggtcacc gggaccggct tcccgcccgg ggagtccatc ggggtcgtca tgtgcaccaa
    39001 ggaggccggc caggatcacg gcgcccgcgg cgtggatgcc tgcaacatcg gccgcttcgc
    39061 gcagaccact gcggacgccg acggcgtggc caccgtcggg ttcgaggtcc gtcgcctcgt
    39121 cgtgctcgac ggccaggagg tggactgcgc gtccgaggcg cagcgctgcc tgatcggcat
    39181 ggggatgatc tccgactacg accagtccgg tggcgtgctc gtcgacttcg acccgagcgt
    39241 gccccttccg gacccaccca cgtccagcat cgaggccccg ggccccttcg acgacggcga
    39301 ggcagtcacg gtccgggtcg acgggctccg ccccggcacc ggtgtgggcg gcacggtgtg
    39361 cactgcggat ggcgcgttct gcaccgacgc tccgtcgaac ggcgtggtcg cagacgacgg
    39421 gaccgcgacc ttcgagctcc ggctgtggcg ggtcttctcg gcgccggtgt gggaggccac
    39481 gaccgccggc caggacatcg actgcgcggt cgtgacctgt cgcctgcagc tctggggtga
    39541 ggcggtcggc gggcgggcca tccccatgct cgacctgtcg ttcaccgatg gcccgatcga
    39601 ccgggtccga cctgtgctcg aggtgcgctc cgagggtccc taccggcccg gcgaccgcat
    39661 cgaggccttc gtccccgatg tcggaccgaa cagcgggctc gagctgctgc tgtgcggtcc
    39721 gcaggcgtgc ggcggcggct cggtcgagct ggactgggtg ttcgggggga tccaggcccg
    39781 cctcaccgtc ccgtcggccg gcgagggcaa cccgtgcatc ggggagccgt gccgcctcgt
    39841 ggcccaggtg tgggccgagc cagtccccgg tgcggcgcct ccgctggcgc cggcaccggt
    39901 cgaggtcgtc gtcgaggcct gaacctgctg gtggctggac tccggcgacc acccaatagg
    39961 atccgctgct cggttccgcc tcccggcgac ggctttctag gagtgccagc gtgacgttca
    40021 aggtcggcga caaggtcgtg tacccccacc acggggccgc cgtcatcgag aagaccgagg
    40081 tgatggagag cttcggggag gagaaggagt tcttcgtcct gcgcatgacg cacggcgagc
    40141 tcaccttgaa ggtgccctcc gaccgcgccg aggagatcgg catgcggtac ccgatctcgg
    40201 acgaggacgt cgaggacgtc ttcgaggtgc tggccaagaa ggacgtccgc gagcccacca
    40261 actggtcccg gcggttcaag aaccaccagg agaagctgaa gtccggcgac gtctaccagg
    40321 tcgccgaggt cgtgcggaac ctggccctgc gggaggctga caagggcctg tccgccggcg
    40381 agaagtccat gttcgtcaag gcccgccagg tcctggtgtc cgagctcgcg ttcgcgctgg
    40441 acatcgagga ggaggatgca ttggcgcggt tggagaccac cctctcgtag cggctgatgc
    40501 ctggcgacga ggagacggag gagaccggcc cgtcggacga gaccatcgcc cggctccgct
    40561 cccgcgccga gcagctgcag gcggcacgtc gcctccgcga gcaggacgag gccgagcacg
    40621 acaccgagcc tgatgccggc gtgcccgccc tcgaggacga ggacgaggac gacgaggacc
    40681 ccttcgcggc tatgctctcg gcgggagccc cgctcgccgg cgccgaggac ctggtcggtg
    40741 gcgccgaccc cctcaccgag cacggcggcg accccgacgc ggagcgcctc ctggaccgct
    40801 gggccgagga ggagcgcgac gaggcggtcg aaccgaagcg gaactggtcc ccgctcatcg
    40861 ccgtggtcgt cgtgctcgcc gtcgccgccc tggcgttcgc cggagcccgg gcgttgatca
    40921 ccggctcggc cgacgacgac accgcgacgg gagggctcct cgacaccgac gacgacggct
    40981 cgtcggtcct cgacgaggac accccgagcc tcgaggacct cacctccgac gtcaccctgc
    41041 cgccgggccc cgatgccggg ctgcaggtcg ccgaccaggg catcaccatc gtgcccgacc
    41101 gcttcgacga gtcccgccgc gagggcacgt tcgcggtgat cgtgcagaac ccgcacgacg
    41161 actggctcgc ccagggcgtc caggtgtcgg cgacgttcct cgacgagggc ggccagccgc
    41221 tcggcgccga cgacggcttc gtggaggtgg tcctgcccgg gcagtccgtg gccgtcgcct
    41281 ccctgttctt cgatgcgccc accgtcccgg tggccggcct cgacgtgcag gtcgaggtgg
    41341 cccgctggcg cgagaccggg ccggtcgccg ggggcttctc gatcaccgag gtgcagacga
    41401 acgaggccga gttcagcggc gttcgcacga ccttcctgat ccagtcggac ttcgacgagc
    41461 cgctcaccga cgtcggcgtg accgcggtct accggaccgc cgacgggcgg atcatcggcg
    41521 gctacgacac cttcgtcgat cggctcgacc ccggcgtggc gaccctggcc gagatcgacc
    41581 tgctggccaa cgtcgacccg gcccagatcg cctcgaccga gctctacccc accgccgcgt
    41641 tcggctccgt ccccgactga gcccggacgc gacgaaggcc cccgaagggg ccttcttccg
    41701 tgggcgtaac aggacttgaa cttccggcga agggtgtgt
//
LOCUS       DATISU010000338         1521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_444283, whole genome shotgun sequence.
ACCESSION   DATISU010000338 DATISU010000000
VERSION     DATISU010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444283"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1521)
                     /locus_tag="VLR27_13470"
     CDS             complement(<1..>1521)
                     /locus_tag="VLR27_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HSP04513.1"
                     /translation="MDGEKGEGRAFGDVESIRRAYEHGDIAIHAKIQYRAGRDEEGKA
                     RWITTTPGRVIFAECLPESFDFLGTMNERLDKRGLSAIVDRLASEYSKAEVAQALDAI
                     KDLSYTWASRSGLTVSIDDVKTPESKRDILDSHEVEADKVETQFRRGIITDGERRQKE
                     IEIWTNATEEIRAEMEIGLKAERFNPIDMMVASGARGNMMQVRQIAGMRGLVANPRGD
                     MIPRPIKSNFREGLDMLEYYIATPGARKGLVDTALRTADSGYLTRRLVDVAQELIVNE
                     EDPFDRAERTGSPVRSIWIEDIRPDLPNKRFYIETKLLGRVLAEDVTLAEPDEEGVSS
                     YERGTMVSELMMQAMIDDPDVTRVRVLSPLTDDSERGISALGYGMSMATGGMIERGEA
                     VGVIAAQSIGEPGTQLTMRTFHTGGVASSSGDIAGGLPRVVELFEARTPKGKATLSTV
                     SGVVRILEDEGRGKTVLVVTDDGEEVSFPIPVGARLEVTDGQELRAGDAIVEGPRDP"
BASE COUNT          210 a          496 c          554 g          261 t
ORIGIN      
        1 ggggtcgcgg ggaccctcga cgatggcgtc gccggctcgc agctcctggc cgtcggtgac
       61 ctcgaggcga gcgccgacgg ggatggggaa ggagacctcc tcaccgtcgt cggtgacgac
      121 gagcacggtc ttgccgcgcc cctcgtcctc gaggatccgg acgacgccgg agacggtgga
      181 cagggtggcc ttgcccttgg gcgtgcgggc ctcgaacagc tcgaccacgc ggggcagacc
      241 gccggcgatg tcgccgctgc tggatgcgac gccaccggtg tggaacgtgc gcatcgtgag
      301 ctgcgtgccg ggctcgccga tggactgggc ggcgatcacg ccgacggcct cgccgcgctc
      361 gatcatcccg cccgtggcca tggacatgcc gtagcccagg gcggagatgc cgcgctcgga
      421 gtcgtcggtg agcggagaca gcacccggac ccgggtgacg tcgggatcgt cgatcatcgc
      481 ctgcatcatc agctccgaga ccatggtgcc gcgctcgtag gagctgacac cttcctcgtc
      541 gggctcggcg agggtgacgt cctcggccag cacccggccg aggagcttgg tctcgatgta
      601 gaagcgcttg ttcggcaggt cggggcggat gtcctcgatc cagatcgagc gcacgggcga
      661 gccggtgcgc tcggcacgat cgaagggatc ctcctcgttg acgatgagct cctgggccac
      721 gtcgacgagg cgacgggtga ggtagccgga gtccgcggtg cgcagcgccg tgtcgaccag
      781 gcccttccgg gcaccaggcg tggcgatgta gtactcgagc atgtcgagcc cctcgcggaa
      841 gttcgacttg atcgggcggg ggatcatgtc accacggggg ttggccacca ggccgcgcat
      901 gcccgcgatc tggcggacct gcatcatgtt ccctcgggca ccggaggcga ccatcatgtc
      961 aatcgggttg aagcgctcgg ccttgaggcc gatctccatc tccgcccgga tctcctcggt
     1021 ggcgttggtc cagatctcga tctccttctg gcgccgctca ccgtcggtga tgatgccccg
     1081 acggaactgg gtctcgacct tgtcggcctc gacctcgtgg ctgtcgagga tgtcgcgctt
     1141 ggactcgggg gtcttgacgt cgtcgatgga caccgtcaga ccggaccggg acgcccacgt
     1201 gtagctgagg tccttgatcg cgtcgagggc ctgggccacc tcggccttgc tgtactccga
     1261 ggcgaggcgg tcgacgatgg cggagaggcc ccgcttgtcg agccgctcgt tcatggtgcc
     1321 gaggaagtcg aaggactcgg gcaggcactc ggcgaagatg acgcggccgg gggtggtggt
     1381 gatccaccgg gccttgccct cctcgtcgcg gccggcgcgg tactggatct tggcgtggat
     1441 ggcgatgtcg ccgtgctcgt aggcccggcg gatcgactcc acgtcgccga aggcgcggcc
     1501 ctcgcccttc tcgccgtcca t
//
LOCUS       DATISU010000339         6387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_769675, whole genome shotgun sequence.
ACCESSION   DATISU010000339 DATISU010000000
VERSION     DATISU010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769675"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1578
                     /locus_tag="VLR27_13475"
     CDS             <1..1578
                     /locus_tag="VLR27_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP04514.1"
                     /translation="VPLIGRTIGEDLERTVHRHPENEALVDVPTGRRWTYRELDAAVD
                     AVATALLQRGIAKGGRVGIWSPNCAEWVFLQYATAKVGAVLVNINPAYRSHELEYVLN
                     QAGIQMLVAAPSFKTSDYAAMIDEVRPRCAALRDVVLLGGPDWDAMASATVDRAAIDD
                     RAATLDVDDPINIQYTSGTTGFPKGATLSHHNILNNGFFVGHLLGYTDADRVCVPVPF
                     YHCFGMVMGNLGATSNGATIVIPAPSFDPAATLQAVQDERCTSLYGVPTMFIAELALP
                     DFADYELSSLRTGIMAGSPCPVEVMKRVVSEMHMEEVTIMYGMTETSPVSTMTRRSDD
                     LDRRVSTVGTVMPFVEIQVVDPATGRNVPRGTPGELCTRGYSVMLGYWDAPEQTADAI
                     DAAGWMHTGDLATMDEDGYVAIVGRIKDMVIRGGENIYPREVEEYLYRHPAISDVQVI
                     GVPDERYGEELMAWVTLKPGESLTIDELRAFCDGQLAHYKIPRHLHVCDEFPVTVTGK
                     VRKVEMRERAAEILGLR"
     gene            complement(1585..1989)
                     /locus_tag="VLR27_13480"
     CDS             complement(1585..1989)
                     /locus_tag="VLR27_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HSP04515.1"
                     /translation="MTRTGRCLCGAVTYELTGDVIATAVCHCDHCQRQSGGAFSVNLV
                     VHESQLAVSGDLQTYVETGENDDGEYVNRKFCGTCGSPIVSELVQTEGVVAVKAGSLD
                     DRSDVRPTVEVWCVDRQPWVELPGMLASVDRE"
     gene            complement(2037..3035)
                     /locus_tag="VLR27_13485"
     CDS             complement(2037..3035)
                     /locus_tag="VLR27_13485"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSP04516.1"
                     /translation="MLAPIAWRTPPVHYGPWELFASLLTEGLVALGHDVTLFATADSI
                     TDATLHATSPAGWSEDGSIDPKVAECLHISAVFERADDFDIIHNGFDFLPLTYSGLVD
                     TPVVTTIHGFSSPAIVPVYERYDATTTYVAISDADRHPRLTYAATIHHGIDVDAFGLH
                     PEPGEHLVFFGRIHPDKGTAAAIEVAHRTGRRLDIAGIVQDEDYFRTEVLPHLDDDRV
                     RYLGPIEATRRSEVLGGAHALLHLIGFDEPFGYSVAEALACGTPVIAHDRGSMGELIR
                     HGVDGFLTADVDTAVHAVEQVGSLDRTRIRASAHERFARATMVRRYVDVYDALLAR"
     gene            3302..4408
                     /locus_tag="VLR27_13490"
     CDS             3302..4408
                     /locus_tag="VLR27_13490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSP04517.1"
                     /translation="MVGTFPPTACGIATFSAALSAGLIAEGHSVDVVRVSPQEGLDDP
                     LVLASLTGPPGPWGTGIEVLDRTDVAIVQHEYGIYPGPDGDEILDVVAQLQVPTVVVA
                     HTVRQTPTSGQRRVLTELCAVASAVVVMTRSGRDRLIDGYGVDPSKVHVIAHGAATPP
                     AATRPAGSRGRPRLLTWGLLGPGKGIEWAIDALLELGAMHPFPEYLVAGATHPKVKEH
                     SGEEYRNMLIQRAWAAGVSPHVSFDDAYRDLPALTELITSADLVVLPYDSDEQVTSGV
                     LVDAVAAGRPVVSTAFPHATELLASGAGIVVPQRDPVALASAIRSVLSDPDLAADMAA
                     EARRLAPGLSWGAVAGEYANLAEHLCTVGRTSTR"
     gene            4405..5436
                     /locus_tag="VLR27_13495"
     CDS             4405..5436
                     /locus_tag="VLR27_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04518.1"
                     /translation="MSVDVLAAPLPRFDHLLAMSDSIGTFEHAEFAAPRRAHGYCTDD
                     MARVLTAVVREPDPSPAVQALGRTAMRFLVEAQDAVGRSRNRRDVDGGWGDRYGVEDC
                     WGRSVAAFGTAANRGADDWTRQMGMASFGHAIEQRSGHFHAMAFAALGAAEVLHADPH
                     NYGARALLEDAVTAIGPPTDDLAWPWPVARLSYANATIAEALIAAGHALHRPDVVEHG
                     CTLLRWLLDRETHDGHLSLTPVDGSGPGDVAPQFDQQPIEAAAMADACARAEAVTGDP
                     SWARGVDLATAWFLGENDVGAVMWDPETGGGYDGIHDAGPNLNQGAESTIALISTLQH
                     ARSRVSVPG"
     gene            5433..>6387
                     /locus_tag="VLR27_13500"
     CDS             5433..>6387
                     /locus_tag="VLR27_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04519.1"
                     /translation="MTADLAVRQPLRLHADPTRVIAKLFVPGHPEPRGGRQVTDAVAH
                     VLELDEARVADGLAEILVRFGSRHRDLASTFEHHAARIANRLPEGTVLSAERRLLLGA
                     TFTHEYSIEAAALCNPSFVAAPNQAGALPGTLDLVMSTRGIGEGHRSSIGFRTASVDA
                     QGHVTMDAPGPFAGAGTLSSGILHADAFRGMARRVRDDIEATTWVLDALGDEFTRTEL
                     DGRLAELEGQDDTRPGVARTVRRLRALAERGYSVDFDPSSPIDERVLYPVTSAEVSGV
                     EDARFVRFEDDDRVVYYATYTAYDGRDIGQQLLETVDFCTFE"
BASE COUNT          916 a         2309 c         2246 g          916 t
ORIGIN      
        1 gtgccgctga tcggtcggac catcggggag gacctcgagc ggaccgtcca ccggcatccc
       61 gagaacgagg cgctcgtcga cgtgcccacc gggcgacggt ggacctaccg cgagctggac
      121 gctgccgtcg acgccgtcgc cacggcgttg ctgcagcggg ggatcgcgaa gggcggccgc
      181 gtgggcatct ggtcgccgaa ctgtgccgag tgggtgttcc tgcagtacgc cacggccaag
      241 gtcggcgccg tgctcgtcaa catcaacccg gcataccgca gccacgagct cgagtacgtc
      301 ctcaaccagg ccggcatcca gatgctggtg gccgccccgt cgttcaagac gagcgactac
      361 gccgcgatga tcgacgaggt gcggccacgg tgcgcggccc tgcgcgacgt ggtgctgctc
      421 ggcggtccgg actgggacgc catggcctca gccaccgtcg accgtgccgc catcgacgat
      481 cgggccgcca cactggacgt cgacgacccc atcaacatcc agtacacgtc cgggaccacc
      541 ggattcccga agggcgcgac cctgtcgcac cacaacatcc tcaacaacgg gttcttcgtc
      601 ggacacctgc tcggctacac cgacgccgac cgggtctgcg tgccggtgcc cttctaccac
      661 tgcttcggga tggtcatggg caacctcggc gccacctcga acggggccac catcgtgatc
      721 ccggcgccgt ccttcgaccc ggcggcgacg ttgcaggcgg tccaggacga gcgctgcacg
      781 tcgctgtacg gggtcccgac gatgttcatc gccgagctcg cgctccccga cttcgccgac
      841 tacgagctgt cctcgctgcg caccgggatc atggccggct ccccgtgccc ggtcgaggtg
      901 atgaagcggg tcgtgtccga gatgcacatg gaggaggtca cgatcatgta cggcatgacc
      961 gagacgtccc cggtgtcgac catgacccgc cgcagcgacg acctcgaccg ccgcgtctcc
     1021 accgtcggca ccgtcatgcc cttcgtcgag atccaggtcg tcgacccggc caccggtcgc
     1081 aacgtcccga ggggcacgcc gggcgagctg tgcacccggg gctactcggt gatgctcggc
     1141 tactgggatg cgccggagca gaccgcggac gcgatcgacg ccgccgggtg gatgcacacg
     1201 ggggacctcg ccaccatgga cgaggacggc tacgtcgcca tcgtcggccg catcaaggac
     1261 atggtgatcc gcggcgggga gaacatctac ccgagggagg tcgaggagta cctgtaccgg
     1321 caccccgcca tctcggacgt gcaggtgatc ggcgtccccg acgagcgcta cggcgaggag
     1381 ctcatggcct gggtgaccct caagccgggc gagtcgctca cgatcgacga gctgcgagcg
     1441 ttctgcgacg gccagctcgc ccactacaag atcccccgcc acctccacgt gtgcgacgag
     1501 ttccccgtga ccgtcacggg caaggtccgc aaggtcgaga tgcgcgagcg agcagcggag
     1561 atcctgggcc tccgctgacg gcggctactc gcggtcgacg gaggccagca tgccgggcag
     1621 ctcgacccag ggctggcggt cgacgcacca cacctcgacg gtcggtcgca cgtcggaccg
     1681 gtcgtcgagc gagccggcct tcacggcgac gacgccctcg gtctgcacga gctcggagac
     1741 gatcggggag ccgcacgtgc cgcagaactt gcggttgacg tactcgccgt cgtcgttctc
     1801 gccggtctcc acgtaggtct gcaggtcccc cgacacggcc agctgggact cgtggacgac
     1861 gaggttcacc gagaacgcgc cgccgctctg acgctggcag tggtcgcagt ggcagacggc
     1921 ggtcgcgatg acgtcgcccg tcagctcgta ggtcaccgcg ccgcagaggc atcttcctgt
     1981 ccgggtcatg gccggatcgt aacggcgggc acccggcacg acggtcgact cccgggtcag
     2041 cgtgcgagga gggcgtcgta gacgtcgacg tagcgccgca ccatcgtggc gcgcgcgaac
     2101 cgctcgtgcg ccgaggcgcg gatccgggtg cggtcgaggg aaccgacctg ctcgacggcg
     2161 tgcacggcgg tgtcgacgtc ggcggtgagg aacccgtcga ctccgtggcg gatgagctcg
     2221 cccatcgagc cgcgatcgtg ggcgatcacg ggcgtcccgc acgccagcgc ctcggcgacg
     2281 ctgtagccga acggctcgtc gaacccgatg aggtggagca gggcgtgggc gccgccgagg
     2341 acctcggatc ggcgcgtcgc ctcgatcggg cccaggtagc ggacgcggtc gtcgtcgagg
     2401 tgggggagga cctcggtgcg gaagtagtcc tcgtcctgga cgatgccggc gatgtccagg
     2461 cggcgtcccg tccggtgcgc gacctcgatg gccgcggcgg tgcccttgtc ggggtggatg
     2521 cggccgaaga acaccaggtg ctcgcccggc tccggatgga ggccgaacgc gtcgacgtcg
     2581 atgccgtggt ggatggtggc ggcgtaggtc aggcgcgggt ggcgatcggc gtcgctgatc
     2641 gccacgtagg tcgtggtggc gtcgtagcgc tcgtagaccg ggacgatggc gggtgaggag
     2701 aagccgtgga tcgtcgtgac gacgggggtg tcgaccagcc cgctgtaggt cagcgggagg
     2761 aagtcgaagc cgttgtggat gatgtcgaag tcatcggcgc gctcgaacac cgcgctgatg
     2821 tgcaggcact cggcgacctt cggatcgatg gagccgtcct cggaccagcc cgcaggcgat
     2881 gtcgcgtgca gcgtggcgtc ggtgatcgag tcggcggtgg cgaacagggt gacgtcgtgg
     2941 ccgagggcga cgaggccctc cgtcagcagg gaggcgaaca gctcccacgg cccgtagtgc
     3001 accggtggcg tgcgccaggc gatcggtgcg agcacggcga tccgcagcgg ggacgtcgtg
     3061 ttccctggtt gcgaggcgat ggtcgagcgt acccaggagg ggtcgggacc ggacggccgt
     3121 aggctcgggg catccctcct tgggggagtc gccatgcttg ggtccggacg gccgagaacg
     3181 gtccgtcacc ctccgcaggc ccgacgcctc gcctgagccg accatcggag cgctcgtgcc
     3241 aggccccttc cattcgccat cgtccatcgg ttccatcgcg cccgcgtccc gcacgttcgg
     3301 catggtcggg accttcccgc cgaccgcctg cggcatcgcc accttctccg ccgccctgtc
     3361 ggccggtctc atcgccgaag ggcactcggt cgacgtggtg cgcgtcagcc cgcaggaagg
     3421 tctcgacgac ccgctggtcc tggcatcgct gaccggcccg cccggaccgt ggggcaccgg
     3481 catcgaggtc ctcgaccgca ccgacgtcgc catcgtgcag cacgagtacg ggatctaccc
     3541 cggcccggac ggggacgaga tcctcgacgt ggtcgcccag ctgcaggtgc cgaccgtcgt
     3601 cgtggcccac acggtgcgcc agacgccgac gagcggtcag cgtcgcgtgc tgaccgagct
     3661 ctgcgccgtc gccagcgcgg tcgtggtcat gacccggagc ggccgtgacc ggttgatcga
     3721 cggctacggc gtcgacccgt ccaaggtcca cgtcatcgcg cacggcgcgg ccaccccgcc
     3781 cgccgcgacc cgacctgccg ggtcccgtgg gcggccccgg ctgctgacct ggggcctgct
     3841 cggaccgggc aagggcatcg agtgggcgat cgacgcgctc ctcgagctgg gtgccatgca
     3901 cccgttcccc gagtacctgg tcgccggtgc cacccacccg aaggtgaagg agcactccgg
     3961 cgaggagtac cggaacatgc tgatccagcg ggcctgggcc gctggggtct cgccgcacgt
     4021 gtccttcgac gacgcctacc gcgacctgcc ggcgctgacc gagctgatca cctccgccga
     4081 tctcgtcgtc ctcccgtacg actccgacga gcaggtcacg tcgggggtcc tcgtggatgc
     4141 cgtcgccgca ggccgtccgg tcgtgtccac ggcgttcccg cacgccaccg agctccttgc
     4201 gtccggggcc ggcatcgtgg tgccccagcg ggaccccgtc gcgctcgcct ccgccatccg
     4261 gtccgtgctg tccgaccccg acctggcggc cgacatggcg gccgaggccc gacgcctcgc
     4321 ccctggcttg tcgtgggggg ccgtggccgg cgagtacgcc aatctggccg agcacctgtg
     4381 caccgtcggg cggacctcga cccgatgagc gtggacgtcc tggccgcgcc cctcccgcgc
     4441 ttcgaccacc tcctggcgat gagcgactcg atcggcacct tcgagcacgc cgagttcgcc
     4501 gcgccgcgcc gcgcccacgg ctactgcacc gacgacatgg cccgggtcct caccgccgtc
     4561 gtccgggaac cggacccgag ccctgcggtg caggcgctgg gccgcacggc catgcgcttc
     4621 ctcgtcgagg cccaggatgc ggtgggacgg tcgcgcaacc ggcgtgacgt cgacggcggt
     4681 tggggagacc ggtacggcgt cgaggactgc tggggccgga gcgtcgcggc gttcggcacg
     4741 gctgcgaacc gaggcgccga cgactggacg cgccagatgg gcatggcgtc gttcgggcac
     4801 gccatcgagc agcgctccgg ccacttccac gccatggcct tcgccgcgct cggcgctgcc
     4861 gaggtcctac atgctgatcc gcacaactac ggcgcacggg cgctgctcga ggatgccgtc
     4921 accgccatcg gcccaccgac ggatgacctc gcctggccgt ggccggtggc ccgactgtcc
     4981 tacgcgaacg ccacgatcgc cgaggcactg atcgctgccg gccatgccct ccaccggccc
     5041 gatgtggtcg agcacggctg cacgctgctc cgctggctcc tcgaccggga gacgcacgac
     5101 gggcacctgt cgctgacgcc ggtcgacggc tccggacccg gggacgtcgc gccgcagttc
     5161 gaccagcaac cgatcgaggc ggcggcgatg gccgatgcgt gcgcacgggc cgaggccgtc
     5221 accggtgacc cgagctgggc gcgcggggtc gacctggcca ccgcgtggtt cctcggcgag
     5281 aacgacgtcg gtgccgtgat gtgggacccc gagaccggtg ggggctacga cggcatccac
     5341 gacgccggac ccaacctgaa ccagggcgcc gaatccacca tcgcgctgat ctccacgctg
     5401 cagcacgccc gcagccgcgt gtcggtgccg ggatgaccgc cgatctggcc gtgcgccagc
     5461 cgctccggtt gcatgccgac ccgacccgcg tgatcgccaa gctgttcgtg cccgggcacc
     5521 ccgagccgcg gggcgggcgg caggtgacgg acgccgtcgc ccacgtgctc gagctcgacg
     5581 aggcccgggt cgcggacggt ctcgccgaga tcctcgtccg gttcggctcc cgccaccggg
     5641 acctggcgag caccttcgag caccacgccg cgaggatcgc caaccggctc cccgagggca
     5701 ccgtcctcag cgccgagcga cggctgctcc tcggtgcgac cttcacccac gagtacagca
     5761 tcgaggccgc cgcgctctgc aacccctcgt tcgtcgctgc gccgaaccaa gccggtgcgc
     5821 tccccggcac gctcgacctc gtcatgagca cccgcgggat cggtgaggga caccggtcct
     5881 ccatcggctt ccgcaccgca tccgtcgacg cgcagggcca cgtcaccatg gacgctcccg
     5941 gaccgttcgc cggcgccggc acgctttcgt cggggatcct ccacgccgat gccttccgcg
     6001 gcatggcgcg acgcgtccgg gacgacatcg aggccacgac ctgggtcctc gatgcgctcg
     6061 gtgacgagtt cacccggacc gagctcgacg gtcgcctcgc cgagctcgag ggccaggacg
     6121 acacccgccc gggcgtggcg cggacggtcc gtcggctgcg ggcgctcgcc gagcggggct
     6181 acagcgtcga cttcgacccg tcctccccga tcgacgagcg cgtgctctac ccggtgacga
     6241 gcgcggaggt gagcggcgtc gaggatgctc gcttcgtccg cttcgaggac gacgaccgcg
     6301 tcgtctacta cgccacctac accgcctacg acgggcgcga catcggccag cagctcctcg
     6361 agaccgtcga cttctgcacc ttcgagg
//
LOCUS       DATISU010000340         4581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_211727, whole genome shotgun sequence.
ACCESSION   DATISU010000340 DATISU010000000
VERSION     DATISU010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211727"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1697)
                     /locus_tag="VLR27_13505"
     CDS             complement(108..1697)
                     /locus_tag="VLR27_13505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP04520.1"
                     /translation="MIAPDLTAAAAAIDAARGVLDKASARLAELGDIDANQVLAYDLA
                     HATAAIEMSATLLDYGAKGDTEARIACAFAADAVGELAAKLFGREAEWGVEPGALDEA
                     REFVGSYRSPEFLTSIEGEGPRHLDGDFEMVQDTFRRFGDDQIKPHAEHVHRTNGDVP
                     EDVISGLAELGAFGLSIPEEFGGFASGGESDYMGMVVATEELSRASLGIGGSLITRPE
                     IMTRAVEKGGTQEQKEHWLPKLATTEVMCAVAVTEPDFGSDVAGVKTTATRDTLDDGT
                     EGWRINGVKTWCTFGARADVLLLLARTDPDRSLGHKGLSMFIVPKPQGEGHGFTFEDG
                     HGGKMDGSPIDTIGYRGMHSYEIALDNWFVPQDNLIGGDDGLGRGFYLQMEGFENGRL
                     QTAARAVGVMQAAYEEAKQYAADRVVFGQPIAEYQLTQAKLAKMAVIIQASRQFSYAV
                     AKMMAKGEGQAEASMVKAYVCKAAEWVTREALQIHGGMGYAEEYTVSRLFVDARVLSI
                     FEGADETLCLKVIARRLTTGN"
     gene            complement(1760..4051)
                     /locus_tag="VLR27_13510"
     CDS             complement(1760..4051)
                     /locus_tag="VLR27_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSP04521.1"
                     /translation="MGVVGGAARARAGGARNLGVPAAMVVVAVVGFAVAGFELASGLS
                     GPRPILLALVGFAAAAMLSAVPISLALSRRAALEAEYADLVQSADAIVWWLAEGADSY
                     GSISAAAGRILGRPQHELLEVDRWHELVHPEDRAALVVARAETLATTTSCEVDYRMLD
                     ADGQWRWLREALTAEPEAPRAPARLRGVVVDVTSRRATEDERALYHEVVEHIETAIVV
                     ARPSDRLDGGLGVIAANPAAAALFGTTPERILGRLVTDLPGAPGDDVVLRAHLVARGG
                     DPFVVERAEAVGVDPGKTFTIRAFGLPGGAVGLGFEDVTAAAMVSAALRRQALHDGLT
                     GLPNRTLLRDRLQTALGEARRRGERVALVLLDLNHFKDVNDALGHQYGDELLMSFAER
                     LRHLLRDCDTIARLGGDEFAVLLTGATDAGSVLVAEKVIEAMAEPFVLHGVTVKSTAS
                     LGIAMYPDDAEDADLLTQRADVAMYAAKRSGGGWRSYSPADDQSSIERLTLLADLHEE
                     LTRSAPETLELHYQPVLDLAGDGITGVEALLRWDHPSFGPLDPEMVVGLAEVSGLVND
                     LARHVVREAITTGRRWRDRGVELCMAVNLSARNLYDAQLTDWISALLRELDVPPSSLK
                     CELTESQLMDDPVLSLEILGRLRDIGVRTAIDDFGTGYSSLSYLRRLPVDEIKIDRSF
                     VLQMADDHNDLMIVRTVIDLAHNLGLRVLAEGVEDERTLQLLRSLGCDEAQGHLVGRP
                     MTADRLLASVRSQASVPLRTSRR"
     gene            4190..4444
                     /locus_tag="VLR27_13515"
     CDS             4190..4444
                     /locus_tag="VLR27_13515"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HSP04522.1"
                     /translation="MAQRGNQSFEKRQREADRQERAREKDARRQERREAEDEGTGEDE
                     GALMAEYGRLAERHDNGEITEEQFVARKREIFEALGLEVD"
BASE COUNT          653 a         1589 c         1688 g          651 t
ORIGIN      
        1 tggtcgaccc ccacgacgat cacgcgggcg ccgacttcga acgcatccgc cacctcggcg
       61 aactgctcga ctggttcggc tgaaacggtc cagcccagtc ggcgtgctca gttgccggtg
      121 gtgaggcggc gggcgatcac cttcaggcac agggtctcgt ccgctccttc gaagatcgac
      181 agcacgcggg catcgacgaa caggcggctc accgtgtact cctcggcgta tcccatgccg
      241 ccgtggatct ggagcgcctc gcgggtgacc cactcggcgg ccttgcacac gtaggccttc
      301 accatggacg cctcggcctg gccctcgccc ttggccatca tcttggccac ggcgtaggag
      361 aactggcggc tggcctggat gatcacggcc atcttggcga gcttggcctg ggtcagctgg
      421 tactcggcga tgggctggcc gaacacgacg cgatcggcgg cgtactgctt ggcctcctcg
      481 tatgcggcct gcatgacacc gacggcgcgg gctgcggtct ggagacgacc gttctcgaac
      541 ccctccatct gcaggtagaa cccgcggccg aggccgtcgt cgccgccgat gaggttgtcc
      601 tgggggacga accagttgtc gagggcgatc tcgtaggagt gcatgccccg gtacccgatg
      661 gtgtcgatgg ggctgccgtc catcttgccg ccgtggccgt cctcgaacgt gaagccgtgc
      721 ccctcgccct ggggcttggg gacgatgaac atcgacaggc ccttgtggcc gagggagcgg
      781 tcggggtcgg tgcgggccag caggaggagc acgtcggccc gggccccgaa cgtgcaccag
      841 gtcttcacgc cgttgatgcg ccagccctcg gtgccgtcgt cgagggtgtc gcgcgtcgcg
      901 gtggtcttca cgccggcgac atcggaaccg aagtcgggct cggtgaccgc caccgcgcac
      961 atgacctcgg tggtggccag cttggggagc cagtgctcct tctgctcctg cgtgccaccc
     1021 ttctcgacgg cccgggtcat gatctcggga cgcgtgatga gcgagccgcc gatgccgagg
     1081 ctggctcggg acagctcctc ggtggccacg accatgccca tgtagtcgga ctcgccgccc
     1141 gacgcgaagc cgccgaactc ctcggggatc gacaggccga aggcgccgag ctcggccagg
     1201 ccgctgatga cgtcctcggg cacgtcgccg ttggtgcggt gcacgtgctc ggcgtggggc
     1261 ttgatctggt cgtcgccgaa gcgtcggaag gtgtcctgca ccatctcgaa gtcgccgtcg
     1321 aggtggcggg ggccctcgcc ctcgatgctg gtgaggaact cgggggagcg gtagctgccg
     1381 acgaactcgc gcgcctcgtc gagtgcgccc ggctccacgc cccactcggc ctcgcggccg
     1441 aacagcttgg cggccagctc gccgacggcg tcggcggcga acgcgcaggc gatgcgggcc
     1501 tcggtgtcgc ccttggcccc gtagtcgagc agcgtggccg acatctcgat ggcggcggtg
     1561 gcatgggcca ggtcgtaggc cagcacctgg ttggcgtcga tgtcgccgag ctcggcgagg
     1621 cgggcggacg ccttgtcgag cacgccccgg gcggcgtcga tggcggcggc ggcggcggtg
     1681 aggtcggggg cgatcatggc ggcagggtac cggtgggtaa ccggcccctt ccaagcaggc
     1741 gagcgacggg ggaccgggtt cagcgccggc tggtgcggag cgggaccgat gcctgcgacc
     1801 ggacgctcgc cagcagccga tccgccgtca tcggccgtcc caccaggtgc ccctgggcct
     1861 cgtcgcaccc gagcgaccgg aggagctgga gcgtccgctc gtcctcgacc ccctccgcca
     1921 gcacgcgcag gccgaggttg tgggcgaggt cgatcacggt ccgcacgatc atcaggtcgt
     1981 tgtggtcgtc ggccatctgc aggacgaacg agcggtcgat cttgatctcg tccacgggga
     2041 ggcggcgcag gtaggacaac gacgagtagc ccgtcccgaa gtcgtcgatc gcggtccgga
     2101 cgccgatgtc gcgcaggcga ccgaggatct cgagggagag caccgggtcg tccatgagct
     2161 gggattcggt cagctcgcac ttgagcgagc tcggcggcac gtcgagctca cggagcagcg
     2221 cgctgatcca gtcggtcagc tgggcgtcgt agaggttgcg ggccgacagg ttcacggcca
     2281 tgcacagctc cacgccccgg tcccgccacc gccggccggt ggtgatggcc tcgcggacca
     2341 cgtggcgggc caggtcgttg acgagccccg acacctcggc cagaccgacg accatctccg
     2401 gatcgagcgg cccgaagctc gggtggtccc agcgcagcag cgcctcgacg ccggtgatgc
     2461 catcaccggc caggtcgagc accggctggt agtggagctc gagggtctcg ggggcggatc
     2521 gggtcagctc ctcgtggagg tcggccagca gcgtgaggcg ctcgatggag gactggtcgt
     2581 cggccggcga gtaggaccgc cagccgcccc cgctgcgctt ggcggcgtac atggccacgt
     2641 cagcccgctg ggtcagcagg tcggcgtctt cggcgtcgtc ggggtacatg gcgatgccga
     2701 ggctcgccgt ggacttcacc gtcaccccgt ggagcacgaa gggttcggcc atcgcctcga
     2761 tgaccttctc cgcgaccagg acggagccgg catcggtggc gccggtcagg agcacggcga
     2821 actcgtcgcc gccgaggcgg gcgatcgtgt cgcagtcacg gaggaggtgg cgcaggcgct
     2881 ctgcgaacga catcaggagc tcgtcgccgt actggtggcc gagggcgtcg ttcacgtcct
     2941 tgaagtggtt caggtcgagc agcaccaggg ccacccgctc gccccgccgc ctggcctcgc
     3001 cgagcgcggt ctgcaggcgg tcgcgcagca acgtccggtt gggcagaccg gtcagcccgt
     3061 cgtggagcgc ctggcgccgc agggccgccg acaccatggc ggccgccgtg acgtcctcga
     3121 agccgagccc gaccgcgcca ccgggcagcc cgaaggcccg gatggtgaag gtcttgcccg
     3181 ggtcgacgcc cacggcttcg gcgcgctcga cgacgaaggg gtcgccgccc cgcgccacga
     3241 ggtgggcccg gagcaccacg tcgtctccgg gagcgccggg caggtcggtg accaggcgac
     3301 cgaggatgcg ctcgggcgtg gtgccgaaca gcgccgcggc cgccgggttg gcggcgatga
     3361 cgccgagccc accgtcgagg cggtcgctcg gccgggccac cacgatggcg gtctcgatgt
     3421 gctcgacgac ctcgtggtac agcgcccgct cgtcctcggt cgcccgccgg ctggtgacgt
     3481 cgacgacgac gccgcgcagg cgggcgggcg cgcgtggtgc ctccggctcg gccgtcagcg
     3541 cctcacggag ccagcgccac tggccgtcgg cgtccagcat gcggtagtcg acctcgcagc
     3601 tcgtcgtggt cgccagcgtc tcggctcggg ccacgaccag cgcggcccgg tcctccgggt
     3661 gcacgagctc gtgccatcgg tcgacctcga gcagctcgtg ctgggggcga ccgaggatcc
     3721 tgccggcggc cgcggagatg gagccgtagg agtcggcgcc ctcggccagc caccacacga
     3781 tggcgtcggc gctctggacc aggtcggcgt actcggcctc cagcgcggcc cggcgcgaga
     3841 gcgcgaggga gatcgggacg gcggagagca tggccgcggc tgcgaacccg accagtgcca
     3901 gcaggatcgg tcgcgggccg gacagccccg acgccagctc gaagccggcc accgcgaagc
     3961 ccacgaccgc gaccacgacc atggcggccg ggaccccgag gttgcgggca ccgcccgctc
     4021 tggcgcgtgc cgcgccgccg accactccca ccctccgcct ccgcatccac cggtgccgtc
     4081 ccgcgacggc gacggcgttc catcggcgcg tcgcgcctcg aactggagca cgaagtcggt
     4141 catcggcgcc cgcgccggta cgttcgacgg tgacgagagg acgatcccga tggcccagcg
     4201 tggcaaccag agcttcgaga agcgccagcg cgaggcggac cgccaagagc gcgctcgcga
     4261 gaaggatgcc cgacgtcagg agcggcgcga ggccgaggac gagggcaccg gtgaggacga
     4321 aggtgccctc atggccgagt acggccgcct cgccgagcgt cacgacaacg gtgagatcac
     4381 cgaggagcag ttcgtcgccc gcaagcggga gatcttcgag gcgctcggtc tcgaggtcga
     4441 ctgacggggg tcgatgggcg tccggcgccg gggcctttgt aggttctccc gccatgagca
     4501 agacgaaccc gggcaacttc ttcgaggact tccgcgtcgg ccaggtcatc gagcacgcca
     4561 ccccgcgcac cgtcaccgac g
//
LOCUS       DATISU010000341        24781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_864070, whole genome shotgun sequence.
ACCESSION   DATISU010000341 DATISU010000000
VERSION     DATISU010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 24781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24781
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864070"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..597
                     /locus_tag="VLR27_13520"
     CDS             238..597
                     /locus_tag="VLR27_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSP04523.1"
                     /translation="MELRSTEVCCQPVTESLLSEDEAEELAGLLKVLADPARLRLLSL
                     VATAEAGEACACDLVEPLGRSQPTVSHHLSLLVDAGLLTREKRGRWAWYRVVPGRLAA
                     IQDALAPSAAVSSSPRP"
     gene            complement(575..1771)
                     /locus_tag="VLR27_13525"
     CDS             complement(575..1771)
                     /locus_tag="VLR27_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP04524.1"
                     /translation="MSASHTNPAVAEAESFLAEHWSPDVDRRAWRELVVDEKWAALRW
                     PSEWYGRDLDDEAAEAVEAVFAAAGAPGPGQDKYNLWAGTLLAHGGDELKARMLRPLL
                     LDEVAMCLLYSEPGAGSDLAAIRTTAVRDGEEWVVNGQKVWTSGAQGADYGMLIARTD
                     WDQPKHRGITFFWFPMRQDGVEVRPLRQATGDARFNEVFLTDARVPDSHRLGELGKGW
                     WVLQTALTYERAVMGVTQRRGSEGSGGATAEAHGRIPVPDTSLVRLARETGRADDPVI
                     RQDLARLHAMRTVNEWNGQRAKAVAEAGGSSPLASLGKLAMSGILHFAGHLQGEILGM
                     EGTLDGDAHPRARDANYSQLNAYFTSIGGGTDQIQRNIIGERILGLPREPEVDRGTPF
                     KDVARS"
     gene            1824..3281
                     /locus_tag="VLR27_13530"
     CDS             1824..3281
                     /locus_tag="VLR27_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HSP04525.1"
                     /translation="MTDAMHWLPDAADASARLLFQRGLALVYLIAFIAARNQFRGLLG
                     SRGLTPIASFVARVPFRRAPSIFHWRSSDRAFLVVAWTGIAVSAAALVGLLDRGPILL
                     TMAAWALLWALYLSIVNVGQRWYSFGWESLLCEAGFLAIFLGPADVEAPAAILVLLWW
                     LLFRLELGAGLIKLRGDPCWRDLTCLQYHHETQPMPNPLSRWFHLLPGPLHRVEVAAN
                     HVAQLVAPVLLFAPQPIRSGAAVLMIVTQAYLMLSGNFAWLNLLTIVIGFAALDGHLI
                     EAVIGLSPPTELAASPVWFDVVVGIVTAVVAVLSIGPAKNLLSKRQRMNASFDPLHLV
                     NTYGAFGSITRTRDEIVIEGTDDPDPGPDTVWREYGFKGKPTDPAQRPRQIAPYHLRL
                     DWLLWFVPLSATYAEPWLAPFLRGLLENDPDICSLLGRNPFPDEPPRAVRARRYRYRY
                     ATRAERRETGNFWTRTPLGEFVRPVTRASLPEDRR"
     gene            complement(3333..5921)
                     /locus_tag="VLR27_13535"
     CDS             complement(3333..5921)
                     /locus_tag="VLR27_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HSP04526.1"
                     /translation="MVQQDSATDLDVVVVGAGFAGMYLLHRLRGVGFSTVVLEAADDV
                     GGTWYWNRYPGARCDIPTVDYTYSFDPELEDEWTWSEKYATQPEILRYAQHVADKHDL
                     RRDIRFDTRVDAARWDEGAARWHVRTEAGDELTCRWYVMATGCLSMPKTPDIEGADRF
                     AGEVHFTSRWPHEGVDFTGKRVGVIGTGSSAIQSIPIIAQQASELTVFQRTPNFSIPA
                     HNGPVAEERKAELEADREAYREAAKWSRGGIPGEPTEISAVLADEETRKQRLDAAWEK
                     GELFEIIGVFNDVLVNPAANEVVADYIRGRIRSIVDDPETAEALCPTDHPFASKRPCL
                     DSGYYATYNLPHVRLVDLRKHPITTITETGIDTADGSHELDAIVYATGFDAMTGALVS
                     VDIEGRDGVTLKQKWADGPTTYLGLTSVGFPNLFTVTGPGSPSVLSNMMVSIEQHVDW
                     IADTLVDLREQGFDVIEPTQTAESGWVQHVNDCADITLYPTANSWYMGANVPGKPRVF
                     LPYVGGVDGYRQACDEVVEAGYLGFELRGDGKVQRNDGVIRRLQPDVAMVLELMAEME
                     LPPMDSMSAEDARAFSEATNAQRPPGPDVGEIVDGTLPGAAGDLRYRLYRPPTPGPHP
                     VVAYFHGGGWVLGSHDSDDPFCRDLCLRADAVIVSVDYRHAPEARFPAAAEDGFAAVR
                     WIAEHPQELGGIPGALAVAGWSAGANVAAVAAQKARDAAAPHISAQVLVNPVTDSDFS
                     RPSYEENGEGYMLTRDLMAWFWDHYADPADRTDPRAAPLRNPDLSGLPPALVVTCEFD
                     PLRDEGQAYAEALDAAGVQVRHLPGRGLIHTSITAVDMIISATDLRAEIGSELGRMVR
                     EGVAVS"
     gene            complement(6013..6636)
                     /locus_tag="VLR27_13540"
     CDS             complement(6013..6636)
                     /locus_tag="VLR27_13540"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HSP04527.1"
                     /translation="MGLLRFSYGTMSSGKSTLALQIHHNLAKQSRHGLLLSLFDREGA
                     RVSSRLGVSATAVEVTPELDLCAVAAQEVAVNGRLDYLVCDEVQFYAVEQVDQLAVVA
                     DELGADVYAFGLLTDFRGVLFDGTKRMFEVADERVELHVEARCWCGARATNNARLVNG
                     ELVRHGETIVVGDTVAADDTPLFGDVVTYELLCRRHFRAGLVAPADD"
     gene            6772..8670
                     /locus_tag="VLR27_13545"
     CDS             6772..8670
                     /locus_tag="VLR27_13545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP04528.1"
                     /translation="MGGGVGTEPDSTIDDDTWRTGPSSAGAQLVARLLEQHGVAALIW
                     CDGAVPWANATALELIAEDQLVEDGTSRLRRLRDAIGDWSVLADLEPGEERAISLELA
                     STRALLTVAAVALRAHDPTNNEVLVTFRAPLERPVDPAHVARLEAMLDSASDVITVID
                     QQGRVRYSNPAAGRITGLYGSVANGTNMVEFVHPEDHADALDAFDRGVLRGEAVEPLV
                     LRIQMADGEWHHTEVSLAGAVELDDGTTGHVVSLRDVSDRVRRDEEANSHRRHLESLV
                     ENIDDVIVILGPDLSVRWTSPGIERFVDAPAYTNVGESAFNDMHPDDVEGVVTAITEA
                     MAEPNGRSRASLRLRHARFGWRWIEAAVVNRLDDPDVEGLVCTLRDVTDQRDEAGELA
                     RLRDQDRDEMSRLREADRLKDQFLATVSHELRTPLTAVRGFGSVLRQQWRRLGDAERD
                     QLLERIVANATSMETMIEQLLDFSRLQAGRVQVELEPLALDQVVTEMVDSLDLHLQEH
                     EVVIETNDLRVLGDRYAFGHVLRNLLTNAAKYSSAGTRIDIEAERLGDTVHLHVRDQG
                     IGIAPEDQRRVFQSFFQSAPALADRRGTGVGLNVARRYAQLQAGGLDLESELGVGTTF
                     TFTLPAAD"
     gene            complement(8689..10524)
                     /locus_tag="VLR27_13550"
     CDS             complement(8689..10524)
                     /locus_tag="VLR27_13550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSP04529.1"
                     /translation="MRRAEAQQGEAQPATGLLTRMERWQLVTTVGLAIVAIALLALAV
                     AHNQHHGPRVRDSIEATRIVRTAHEAMLDQHAGLRAYALTGDDGFLEAFRNAQEVLDR
                     TGAEQLPADDDIRQRHLDLRLAQAAWTDRWVPDALELGAGGGDGAETGEALLLDGKEL
                     FDDYRSEYVALVGALQDRREDALDGQTEAVTRTTALAVALAVASLALTLVQGRRLRAV
                     VKGALDAVQLRLDRVAAGDLEPGPAPSGPSELADIQRGIDETAAQLASARAAVDVQGD
                     QLRAQNRQLGQVLRFAREVAGSLNLRYVIRGLCSATTNITDSDRVVVWLRSTDDQTLE
                     AIADSSGPALEPVGLEPVPIGEGSIGRAARFGRIEGQPKGSVGRALDDDPEAGGTVAV
                     PMVVGAEVIGVIEVAFPPDAEEPDSTMRVLEALATQAATAVGAARVHEHTETMAMTDA
                     LTQLPNRRRMETDLDREVGASHRYGRPLGFAMIDVDRFKDYNDALGHQAADVALQTIA
                     RILSASVRGSDTVYRYGGEEIAVVMRETDLDSACRLGERLREAVEHHFASPGQPRPVT
                     ISVGVASMPVHAASLPALVGAADQALYEAKRSGRNRTVIAGPDAT"
     gene            10614..11225
                     /locus_tag="VLR27_13555"
     CDS             10614..11225
                     /locus_tag="VLR27_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HSP04530.1"
                     /translation="MPDDVTAYDPQTALVVVDVQNDFADPGGSLYVRGGQEIIPAVND
                     EIAAARAGGATVVTTQDWHPPTTPHFTTDGGTWPVHCVRATWGAELHPELDVEADLVV
                     RKGTGGEDGYSAFTVLDPDTGDARPTGLAGYLRERGVRRAVVVGLAADVCVKATALDA
                     EAIGLDTTVRWDATRAVDVEPGDGDRALAELRDAGVRIVGAEP"
     gene            11222..12781
                     /locus_tag="VLR27_13560"
     CDS             11222..12781
                     /locus_tag="VLR27_13560"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HSP04531.1"
                     /translation="MTDAGPPRWHRSALFTDSYQLVMAQLYVEDGIADRTAQFDHLYR
                     SNPDYGSHQAGFCVFAGLEPMLEWMATVAVTDADLTALAAQRNPAGDRRYSDDFLGWL
                     SEHGHFRDLEVQAVAEGRVVHPNVPMMTVTGPFAAAQLLETALLNQCNYPTLIATKAT
                     RIVESARGGDVLEFGMRRGPGAAVDEAARAALIGGCSATSNVQASVALGTDPKGTHAH
                     SLVQAHLALGSGELGAFRSFARRYPDECILLVDTVDTLRSGVPNAIQVFEELRAAGHE
                     PAGIRLDSGDLAHLAVESARMLDDAGFERARIVLSGDLDEVTIWQVRTQVIDEAPKAG
                     LDPDALCRRLVYGVGTRLITSEGDPALGGVYKLTAITDETGAWLPAVKLSENPAKVPI
                     TGPKACWRLHDRRGRATVDLMTAPDEVPFDGVDRMTVHHPAQSGVRREIHRDDITRVE
                     PLHDVVFADGRATRPPSTLDELRARRRADVDDLDPGVRRMVNPHRYHVSLSDRLHRIQ
                     QETVERLRAGR"
     gene            12834..14345
                     /locus_tag="VLR27_13565"
     CDS             12834..14345
                     /locus_tag="VLR27_13565"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04532.1"
                     /translation="MTRTSRLAAAVVAVLVLFVPESSAEESDAVHSPNLVPVANLAYE
                     DRYGTGANQGTDIEFARLKHKGKVRDFALAGSYDNGLQIIDVSDPSAPTVVGAYDCGI
                     SQGDVQVFTRGKRTLATYTMDAGYTLQEESACVREAKALGLFEPTTSHPLDIDPIGAI
                     APDSAYGRAGVGTYIVDITDPTRPTTVSFVGVPKGSHNQTVHPSGDYLYNSNSQLYTT
                     ALNAGIEVYDIRDLGAPRLAAVLPLPPVPGLGSESHDITFNEQGTRAYSAALSHTAII
                     DTTVVDRPEIISTIVDPTINVHHQSTKVTLTDANLGVTRDFLLIEDELAGAAGFDPCP
                     SGGLHIYDVTGPLEAAPVKLGYWNIADLRYPTAEGNVGAQCTMHVFQVFPEEGILTAA
                     FYNGGVRVVDISSLVGVALGATGVGMQEIAHARFPDSLTWAAKTPRIDPDGAFHLFGN
                     DLNRGLDVYRFDPTAERSDGLSTWLAAGEVPIPEATTASSDDTLFCLLGRNAS"
     gene            14369..15307
                     /locus_tag="VLR27_13570"
     CDS             14369..15307
                     /locus_tag="VLR27_13570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HSP04533.1"
                     /translation="MHAVGVNSFGGPDALEMVELPDPVAGPGQLLVRVHAATVNPTDT
                     YVRNGARAEHQRKDPPPYVPGMDVAGVLEAIGDGAETDLQVGDRVMAVVLPDGAHGGY
                     CQRLALPAGSVARMPAGASFAEASTIPMNGLTARLALDELDVASGGTVAVTGAAGAFG
                     GYCVQLAKADGLRVIADASEADEQLVRDLGADIVVRRGDGVAERILEHAPDGVDGLCD
                     GSVQDEALFPAIRDGGGFAAVRGLKVEAPRGIHMHRVWVSRYLEEQAELDRLRQQVED
                     GQVAMRVAQTFPMDQAAEAHRLLEAGGIRGRLVIEL"
     gene            complement(15394..16083)
                     /locus_tag="VLR27_13575"
     CDS             complement(15394..16083)
                     /locus_tag="VLR27_13575"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HSP04534.1"
                     /translation="MNRFRLIRVAGLALSLSLVAAACGDDDDTDTSADAPVEETTDEM
                     SEDMGDDMGDESAAPADSAEACDADAIVAAVEGGPEEGTLAGMTDDPVGTAASSNPVL
                     TTLVTAVTEAELVDTLNSAEALTVFAPTDCAFAALDPATLDAALADPSGLLTQVLGFH
                     VIAGEQLSAADLAGVTELETFTGETLEISADGETVSVGGGQANVVVADIQTANATVHL
                     IDSVMLPPSAG"
     gene            complement(16166..17698)
                     /locus_tag="VLR27_13580"
     CDS             complement(16166..17698)
                     /locus_tag="VLR27_13580"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSP04535.1"
                     /translation="MENAPRTPTLPAALWASAAAVAALGAAELAAGVVQTWRSPVVAV
                     AESVIDRSPSALTRFGIRTFGTNDKAALIIGILLILAVVAPLLGVAARRRPWAGVAGF
                     ALFGVVGVAASIDGGPAAAAVPAVLAALIGASTMLVALRPRSGERRIPSDAGVGRREM
                     LAGAGALTALGLVMAAGGRALRDRFSAAASRAALVLPGARQPLPSAPAGASFDGIDGL
                     APLFTPNDRFYRIDTALLIPQVPVEDWTLRVHGMVDEPFEIGFDELVGLGLVEADITL
                     TCVSNRLGGDLVGNARWLGVPLRLLLERAGVQDRADQIVGRSVDGYTCGFPVEAAFDR
                     DALVAVGMNGEPLPLQHGFPARLVTPGLYGYVSATKWLAEIELTRFDTFTQYWVPRGY
                     AERAPIKTMSRIDVPRSGQIVEAGDTVIAGVAWAQTRGISKVEVSIDDGPFAEAELAD
                     ALTDDTWRQWRMPWTATAGAHRLAVRCTDGDGQLQREERDEPLPDGASGWQSLLVTVR
                     EP"
     gene            complement(17747..19255)
                     /locus_tag="VLR27_13585"
     CDS             complement(17747..19255)
                     /locus_tag="VLR27_13585"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF004531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HSP04536.1"
                     /translation="MDHDRTAGTAGVVPVTQARDLDPSLVGAKAASLARMIDLGLRVP
                     PAFVVTTDVCRRVLADGQVPRATIDAVRAAVADLEEATGRRFGGDDPLLVSVRSGAAI
                     SMPGMMDTVLDVGFAPHTRTALADRAGDAFARSCHARFLTGWASIVLGATPQRGDDPD
                     ELAAALAGAVPDDPHDQLLDAIVAVFRSWENERARAYRERHGLDDAGGTAVVVQAMVF
                     GNRGAGGGAGVASSRDPSTGAPALCGDLLVDAQGEDVVAGSHRPRPIDDLGRLWPRVD
                     GELRAAVAAIEAATGDMVDVEFTIEDGTLHLLQSRVGARAAPAAVRIAVDLVEEGVIS
                     VAEALARVDQDQLRHASQPTVDPDGADVLATGLGACPGVATGEVCLSPEHVSSHDGAV
                     VLVRRETSPDDIRGMVASAGVLTARGGLVSHAALVARELDLPAVVGVEALEVDEAAGT
                     VRAGGQVLRDGDIVTIDGTTGRVHAGEPPILAPAPSGHLARFRSWRAEHEPD"
     gene            19312..20718
                     /locus_tag="VLR27_13590"
     CDS             19312..20718
                     /locus_tag="VLR27_13590"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HSP04537.1"
                     /translation="MLPIFAADALLPARYQMAVSLGWHILIAAFGVAFPAIIFVAHRR
                     GLRGDPAGLVLAQRWAKVSGVLFALGAVSGTILSFELGLLWPGMMGTFGDVIGPAFAW
                     EGLAFFTEAIFLGIYLYGFNRLAPRTHLLTLVPVVLAGILGSFSVIAANAWMNSPAGF
                     DIVDGTVTDIDPVAAIFNEAVWLQWIHMLFAAYMVVGFTVAGVYALGRLRGRDDGAHR
                     LGFIVPFAFASVATLLQPVVGHLAGERLAEAQPSKFAAIELLPETTDGAPLTIGGVLI
                     DGEVRYGIQIPNLGSLIATGSPDARIVGLDDVPEGAEVPASIVHLSFQLMVGIGFSLV
                     ALVLWYWFRRWRGSDPLDRSTWFRRGVVVAGPAAMTALIAGWIVTEVGRQPWIVYRVM
                     RVDEAVTDAGWIWWSLTTIVVVYASMTVFGAVVLRAMSRRWREGGEMRVPYGPDTEDD
                     EAGVDVSAGVPGPGGDGS"
     gene            20715..21707
                     /locus_tag="VLR27_13595"
     CDS             20715..21707
                     /locus_tag="VLR27_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HSP04538.1"
                     /translation="MSADLVAAAMFAGVVLYALFGGADYGAGVWDLTAGRGDMADRAR
                     RQIDRSIGPVWEANHVWLIFVIVMLWTGFPRAFSAIMTTLAVPWSFAGLGIVFRGGAF
                     VFRKSSPTYAQAQLHGVVFAASSVVTPFFLGTIAGAIAAGRVPIDGGGDPLTSWTGPL
                     SIVGGVLAVLVTAFLAAVLLTVDADRRGDHELSEWFRRRALLAAAVTGVAALTAAATI
                     EATAPSLADGLHGPGAPLVVASAVGGVTTAWALLRRRLAVARIGAFVAVAAVVTGWGV
                     AQYPDVLVDVATIDDVAGAPVTMRMLLVVVAAAGVIVVPALVWLLRLVDQPRWK"
     gene            complement(21714..22997)
                     /locus_tag="VLR27_13600"
     CDS             complement(21714..22997)
                     /locus_tag="VLR27_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP04539.1"
                     /translation="MAWDFQTEPEFQEKLDWVENFCKEKVEPLDYVFPYAVRSPDPVV
                     KAYVRELQQEVKDQGLWAIFLDEELGGPGFGQLKLGLLNEVIGRYSGAPQMFGAAAPD
                     TGNMEMLAAYGTEEQKKRWLEPLLNQEMFSAYSMTEPQGGSDPNLFRTTAVRDGDEWV
                     INGEKWFTSAGRVADILFVMCTNGMFVVPRETPGVEIQPQPRNHNHIIYNDVRVPADH
                     LLGPEDGAKVLAQRRLGGGRIHHAMRTIAQCTLAFDMMCERALSRESHGKIIGEHQMV
                     QEKIAESYAKLKMLRLYVLETAWKMDQTSSAETRTDIAAVKFTMARVLREISFDALHI
                     HGSLGTTDLTPLQAMYAGAPTMGLADGADEVHKSTVARRVLKDYRPHEGYFPREFIPY
                     KKEEAWEKIQPVLDARPELRESAENYKRYLAKRGR"
     gene            complement(23052..23669)
                     /locus_tag="VLR27_13605"
     CDS             complement(23052..23669)
                     /locus_tag="VLR27_13605"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP04540.1"
                     /translation="MASPVPDALIRAAERLFAERGSDQVSLREITAEAGATNASAVQY
                     HFGDRRGLIRAVLAKHDVDVERRRHALLDAYEEAGTADIRRLAHALVQPLADELGNED
                     GGPGYLQLLSDLFNRPNPPYEPAAAEDPTYSVNRWRALVMPMLTPEAIRLHRRFDALR
                     FTVSELARRGRTGRKDHRLFTSQLVDLVTGLLLAPVSEDTRRLLR"
     gene            complement(23755..24438)
                     /locus_tag="VLR27_13610"
     CDS             complement(23755..24438)
                     /locus_tag="VLR27_13610"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP04541.1"
                     /translation="MSTLRLRQVALVATELDPVLEALGTELGLEVAHRDPAVATFGLV
                     NAVLPVGRQFLEVVSPTRDGTAAGRQLERMGGDGGYMVICQTDDQAPFRQRAEELGVR
                     TAFEFDDDGYQGWQLHPGDTGGSFLEIDVQPAGEDDPWMPAGPTWQQAVRTDVVDGIT
                     AVELEVPEPDATARRWRELLGRKHFDDGVELRWATGERGLVAVDLHASDRARAGDGRT
                     ICGVRFRLV"
     gene            complement(24452..>24781)
                     /locus_tag="VLR27_13615"
     CDS             complement(24452..>24781)
                     /locus_tag="VLR27_13615"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSP04542.1"
                     /translation="TGAALDMLWSSRKVEAAEALRLGLVQQVVAPDDLLDAARRYVVD
                     LAENVSPASLADTKRLVYEGYGVTYPDALDEIDGVQYAALDRPDAREGAAALIEKRAP
                     SFPRLGG"
BASE COUNT         3231 a         9065 c         8940 g         3545 t
ORIGIN      
        1 accagcttca ggggcgggtc ggcgatggcg aagttggcgt agccgggctt gcgcttgagc
       61 ggctcggtgg cgaagagctt ggcgtagaac gccacggcct cgtcgatgtc gctgacgttg
      121 agggcgagct ggactcggga cacgggatcc tcctggtacg gttcatcgac gtccgtcgat
      181 gtctggtgca ccataccaac acatcgacgg ttgtcaatgt gagatcgagg aggtcccgtg
      241 gagctgcgat cgaccgaggt gtgctgccag ccggtgaccg agtcgctgct gtccgaggac
      301 gaggccgagg agctggccgg gctgctcaag gtcctcgccg acccggcccg cctccggctg
      361 ctgtccctcg tggccaccgc cgaggccggc gaggcgtgcg cgtgcgacct ggtcgagccg
      421 ctcggccgct cgcagccgac cgtgtcccac cacctctccc tcctcgtgga cgccggcctg
      481 ctcacccggg agaagcgggg acgctgggcc tggtaccggg tcgtccccgg gcgcctggcc
      541 gccatccagg acgccctggc gcccagcgcg gcggtcagct cctcgccacg tccttgaacg
      601 gggtgccccg gtcgacctcg ggctcccggg gcaggccgag gatgcgctcc ccgatgatgt
      661 tgcgctggat ctggtcggtg cccccgccga tcgaggtgaa gtaggcgttc agctgggagt
      721 agttggcgtc ccgggcccgg gggtgcgcgt cgccgtcgag ggtgccctcc atgccgagga
      781 tctccccctg caggtgaccg gcgaagtgga ggatgccgga catggccagc ttgccgagcg
      841 aggccagggg cgaggacccg cccgcttcgg cgacggcctt ggcccgctgc ccgttccact
      901 cgttcaccgt gcgcatcgcg tggagccggg ccaggtcctg gcggatcacc gggtcgtcgg
      961 cgcggccggt ctcccgggcc aggcggacca gcgaggtgtc gggcaccggg atccggccgt
     1021 gggcctcggc ggtggcgccc ccgctgccct cgctgccgcg gcgctgggtc acgcccatca
     1081 ccgcccgctc gtaggtgagc gcggtctgca gcacccacca gcccttgccg agctcgccga
     1141 ggcggtggct gtcgggcacc cgggcgtcgg tgaggaacac ctcgttgaac cgggcgtcgc
     1201 cggtggcctg gcggagcggg cgcacctcga cgccgtcctg gcgcatcggg aaccagaaga
     1261 acgtgatgcc gcggtgcttg ggctggtccc agtcggtgcg ggcgatgagc atcccgtagt
     1321 ccgcgccctg ggccccggag gtccagacct tctggccgtt gaccacccac tcctcgccgt
     1381 cgcgcaccgc ggtggtgcgg atcgccgcca ggtcggagcc ggcgccgggc tcgctgtaga
     1441 gcaggcacat ggccacctcg tcgagcagga gcggtcgcag catgcgcgcc ttgagctcgt
     1501 cgccgccgtg ggccagcagc gtgccggccc agaggttgta cttgtcctgc cccggcccgg
     1561 gcgcgccggc tgcagcgaag accgcctcga ccgcctcggc cgcctcgtcg tccaggtcgc
     1621 ggccgtacca ctcgctcggc cagcgcagcg ccgcccactt ctcgtccacg accagctccc
     1681 gccacgctcg acggtcgacg tcgggtgacc agtgctcggc gaggaacgac tcggcctcgg
     1741 ccaccgcggg gttcgtgtgc gacgcgctca tggctgcagg ttctcacgcc cgtcagggcg
     1801 tgctccacac cgcggatggg taggtgaccg acgcgatgca ctggctcccc gacgccgccg
     1861 atgcgagcgc ccggctgctc ttccagcgcg gcctggcgct cgtgtacctg atcgcgttca
     1921 tcgccgcccg caaccagttc cggggcctgc tcggctcccg cggcctcacg cccatcgcct
     1981 ccttcgtcgc ccgcgtcccg ttccgccggg ccccgagcat cttccactgg cgcagctcgg
     2041 accgggcctt cctcgtggtg gcctggaccg gcatcgccgt gtcggccgcc gccctggtcg
     2101 ggctcctcga ccgcggcccg atcctgctca cgatggcggc gtgggcgctc ctgtgggcgc
     2161 tgtacctgtc gatcgtgaac gtgggccagc gctggtactc cttcggctgg gagtccctgc
     2221 tctgcgaggc cgggttcctg gcgatcttcc tcggccccgc cgacgtcgag gcaccggccg
     2281 cgatcctggt gctcctctgg tggctgctgt tccgcctcga gctcggcgcc ggcctcatca
     2341 agctgcgcgg cgatccgtgc tggcgggacc tcacctgcct ccagtaccac cacgagaccc
     2401 agccgatgcc gaacccgctg agccgctggt tccacctcct ccccggtccg ctccaccggg
     2461 tcgaggtggc ggcgaaccac gtcgcccagc tggtcgcacc cgtgctgctg ttcgcgccgc
     2521 agccgatccg ctccggcgcc gcggtgctga tgatcgtcac ccaggcgtac ctgatgctca
     2581 gcggcaactt cgcctggctc aacctgctca cgatcgtgat cggcttcgcc gcgctggacg
     2641 gccacctcat cgaggccgtg atcgggctct cacctccgac cgagctggcc gcgtcgcccg
     2701 tctggttcga cgtcgtcgtc ggcatcgtga ccgccgtcgt ggccgtcctg tcgatcggac
     2761 cggcgaagaa cctcctgtcc aagcgccagc ggatgaacgc cagcttcgac ccgctccacc
     2821 tcgtcaacac ctacggcgcg ttcggttcga tcacccgcac ccgggacgag atcgtgatcg
     2881 agggcaccga cgaccccgac cccggcccgg acaccgtgtg gcgggagtac gggttcaagg
     2941 gcaagccgac cgacccggcc cagcggcccc gccagatcgc cccctaccac ctgcgcctcg
     3001 actggctcct gtggttcgtg ccgctgtccg cgacctacgc cgagccgtgg ctcgccccgt
     3061 tcctgcgcgg cctgctggag aacgaccccg acatctgctc gctgctgggt cgcaacccgt
     3121 tccccgacga gccaccccga gcggtgcgcg cccgtcggta ccgctaccgg tacgcgaccc
     3181 gcgccgagcg gcgggagacc ggcaacttct ggacgcgcac gcccctcggc gagttcgtcc
     3241 gcccggtcac ccgagcctcg ctgcccgagg atcggcgctg aacggagcgc atccgcccgt
     3301 ccagcgccga tcggcggcga gccgcacccg gctcaggaga cggcgacgcc ctcgcgcacc
     3361 atgcgcccga gctccgagcc gatctcggcg cggaggtccg tggccgagat gatcatgtcc
     3421 acggccgtga tcgaggtgtg gatcaggccc cgaccgggca ggtgccggac ctgcacgccg
     3481 gcggcgtcga gcgcctcggc gtaggcctgg ccctcgtcgc gcagcgggtc gaactcgcag
     3541 gtcacgacca gcgccggcgg caggccggac aggtcggggt tgcgcagggg cgcggcgcgc
     3601 gggtcggtgc ggtcggccgg gtcggcgtag tggtcccaga accaggccat gaggtcgcgg
     3661 gtgagcatgt agccctcgcc gttctcctcg tagctcgggc gactgaagtc cgagtcggtc
     3721 accgggttca ccagcacctg ggcgctgatg tggggtgccg cggcgtcccg cgccttctgg
     3781 gccgccaccg cggcgacgtt ggcgccggcg ctccagccgg ccaccgccag cgcgccgggg
     3841 atgccgccga gctcctgggg gtgctcggcg atccaccgga cggcggcgaa gccgtcctcg
     3901 gcggcggccg ggaagcgggc ctcgggggcg tgccggtagt cgaccgagac gatcaccgcg
     3961 tcggcccgca ggcacaggtc gcggcagaac ggatcgtcgg agtcgtggct gccgagcacc
     4021 cacccgccgc cgtggaagta ggccacgacc gggtgcggtc cgggcgtcgg gggccggtac
     4081 agccggtagc ggaggtcgcc ggcggcaccg ggcagcgtgc cgtcgacgat ctcgcccacg
     4141 tccggaccgg gtggccgctg ggcgttggtc gcctcggaga aggctcgggc gtcctcggcc
     4201 gacatcgagt ccatcggcgg cagctccatc tcggccatga gctcgaggac catggccacg
     4261 tcgggctgca gccggcggat cacgccgtcg ttgcgctgca ccttgccgtc accgcggagc
     4321 tcgaagccga ggtagccggc ctcgaccacc tcgtcgcacg cctgccggta gccgtcgacg
     4381 ccgccgacgt agggaaggaa gaccctgggc ttcccgggca cgttggcgcc catgtaccag
     4441 gagttcgccg tcgggtagag cgtgatgtcg gcgcagtcgt tgacgtgctg cacccagccc
     4501 gactccgcgg tctgggtcgg ttcgatgacg tcgaagccct gctcgcgcag gtcgacgagc
     4561 gtgtcggcga tccagtccac gtgctgctcg atcgacacca tcatgttgga cagcaccgac
     4621 gggctccccg gcccggtgac cgtgaacagg ttggggaacc cgaccgaggt gagaccgagg
     4681 tacgtggtgg ggccgtccgc ccacttctgc ttcagggtga cgccgtcgcg gccttcgatg
     4741 tcgaccgaca cgagcgcgcc ggtcatggcg tcgaagccgg tggcgtagac gatggcgtcg
     4801 agctcgtggg atccgtccgc cgtgtcgatg cccgtctcgg tgatggtcgt gatcgggtgc
     4861 ttgcgcaggt cgaccaggcg cacgtggggc aggttgtagg tggcgtagta cccgctgtcg
     4921 aggcaggggc gcttggaggc gaaggggtga tcggtcgggc acagggcctc ggccgtctcg
     4981 gggtcgtcca cgatggagcg gatccggccc cggatgtagt cggcgacgac ctcgttggcc
     5041 gccgggttga cgagcacgtc gttgaacacg ccgatgatct cgaacagctc gcccttctcc
     5101 cacgccgcgt ccaggcgctg cttgcgggtc tcctcgtcgg ccagcaccgc gctgatctcg
     5161 gtgggctcgc ccgggatgcc gccgcgcgac cacttcgccg cctcccggta ggcctcgcgg
     5221 tcggcctcga gctcggcctt gcgctcctcg gccacggggc cgttgtgcgc cgggatcgag
     5281 aagttgggcg tgcgctggaa gacggtgagc tcgctcgcct gctgggcgat gatcgggatg
     5341 gactggatcg cggacgaacc ggtgccgatg acgcccaccc gcttgccggt gaagtcgacg
     5401 ccctcgtggg gccagcggct ggtgaagtgc acctcgccgg cgaagcggtc ggcaccttcg
     5461 atgtcgggcg tcttcggcat cgacaggcag ccggtggcca tgacgtacca gcggcaggtc
     5521 agctcgtcgc cggcctcggt gcggacgtgc cagcgcgccg cgccctcgtc ccagcgtgcg
     5581 gcgtcgaccc gcgtgtcgaa gcggatgtcg cggcgcaggt cgtgcttgtc ggcgacgtgc
     5641 tgggcgtagc ggaggatctc gggctgggtg gcgtacttct ccgaccaggt ccactcgtcc
     5701 tcgagctcgg ggtcgaagct gtacgtgtag tcgaccgtcg ggatgtcgca gcgcgccccg
     5761 gggtaccggt tccagtacca ggtgccgccg acgtcgtcgg ccgcctccag gaccaccgtg
     5821 gagaacccga ctccgcgcag gcggtgcagc aggtacatgc cggcgaagcc tgcccccacc
     5881 accaccacat cgagatcggt cgcgctgtcc tgttgcacca tccggtgacc cccttcgtgc
     5941 gtccccgtcg ccagcttggc aacgtccgtg tccagtgtca cacaccgggc gcgcggaacg
     6001 ctgaccgacg ggtcaatcgt ccgcgggtgc gacgagaccg gcccggaagt ggcggcggca
     6061 gagcagctcg taggtcacca cgtcgccgaa caggggggtg tcgtcggcgg ccacggtgtc
     6121 gccgaccacg atcgtctccc cgtggcggac cagctcgccg ttcacgaggc gggcgttgtt
     6181 ggtcgcccgg gccccgcacc agcagcgcgc ctccacgtgc agctcgaccc gctcgtcggc
     6241 cacctcgaac atccgcttgg tgccgtcgaa gagcacgccg cggaagtcgg tgagcagccc
     6301 gaaggcatag acgtcggcgc ccagctcgtc ggcgacgacc gcgagctggt cgacctgctc
     6361 caccgcgtag aactggacct cgtcgcacac gaggtagtcg aggcggccgt tcacggccac
     6421 ctcctgggcg gccacggcgc acaggtcgag ctcgggcgtg acctcgacgg cggtggccga
     6481 cacgccgagg cggctcgaca cccgggcccc ttcccggtcg aacagcgaca ggagcagccc
     6541 gtgccgggac tgcttggcca ggttgtggtg gatctgcagg gccagcgtgc tcttgcccga
     6601 gctcatcgtg ccgtaggaga agcggagcag gcccatggcc ggggacggta gcgagccgct
     6661 ccgtcgccgc cggtgccccg agcgaaccgc ccgggacggc acaagcgcgt cgaagcggga
     6721 tccttgggcc acgaccacgc gcgtcacgac ccagagcgcg aacatgacca catggggggt
     6781 ggcgtgggga ccgagccgga ttcgacgatc gatgacgaca cctggcgcac cggtccgtcg
     6841 agcgccggcg cccagctggt cgcccgactg ctggagcagc acggcgtcgc cgccctgatc
     6901 tggtgcgacg gggcggtgcc ctgggccaac gccacggcgc tggagctgat cgccgaggac
     6961 cagctcgtcg aggacgggac gagccgcctg cgacgcctcc gcgacgccat cggcgactgg
     7021 agcgtgctgg ccgacctcga gccgggcgag gagcgcgcca tctcgctgga gctggcgtcc
     7081 acgcgcgcgc tgctcaccgt cgccgccgtg gcgctgcgcg cccacgaccc gaccaacaac
     7141 gaggtgctgg tcacgttccg ggcgccgctg gagcgccccg tcgacccggc ccacgtggcc
     7201 cgcctcgagg ccatgctcga ctcggccagc gacgtcatca cggtcatcga ccagcagggt
     7261 cgggtgcgct actcgaaccc ggccgccggc cggatcacgg gcctgtacgg gtcggtcgcc
     7321 aacggcacga acatggtcga gttcgtccac cccgaggacc acgccgacgc gctcgacgcc
     7381 ttcgatcgcg gcgtcctgcg cggcgaggcc gtggagccgc tcgtcctgcg gatccagatg
     7441 gccgacgggg agtggcacca caccgaggtc tccctcgccg gtgcggtcga gctggacgac
     7501 ggcacgaccg gtcacgtcgt gtccctccgc gacgtctccg accgggtccg acgggacgag
     7561 gaggccaaca gccaccgccg ccacctcgag tccctcgtgg agaacatcga cgacgtgatc
     7621 gtcatcctcg ggcccgacct gtcggtgcgc tggaccagcc ccggcatcga gcgcttcgtg
     7681 gacgcccccg cctacaccaa cgtcggggag agcgcgttca acgacatgca ccccgacgac
     7741 gtcgaggggg tggtgaccgc catcaccgag gcgatggccg agcccaacgg ccgatcccgg
     7801 gcgtcgctgc gcctccgcca cgcccgcttc gggtggcggt ggatcgaggc ggccgtcgtc
     7861 aaccggctcg acgaccccga cgtcgagggc ctggtgtgca cgttgcgcga cgtcaccgac
     7921 cagcgcgacg aggccggcga gctggcccgc ctccgggacc aggaccgcga cgagatgagc
     7981 cggctgcggg aggccgaccg gttgaaggac cagttcctcg ccaccgtctc ccacgagctg
     8041 cgcaccccgc tgaccgccgt gcggggcttc ggctcggtgc tgcgccagca gtggcgccga
     8101 ctcggggatg cggagcggga ccagctgctc gagcgcatcg tggcgaacgc gacgtcgatg
     8161 gagacgatga tcgagcagct cctcgacttc tcccggctgc aggccggacg ggtgcaggtc
     8221 gagctcgaac cgctggccct cgaccaggtc gtgaccgaga tggtcgactc cctggacctg
     8281 cacctccagg agcacgaggt cgtgatcgag accaacgacc tccgggtcct cggcgaccgc
     8341 tacgcctttg gccacgtcct gcggaacctg ctcaccaacg ccgcgaagta ctcgagcgcc
     8401 ggcacccgca tcgacatcga agccgagcgc ctcggcgaca cggtgcacct gcacgtccgg
     8461 gaccagggga tcggcatcgc gcccgaggac cagcgacggg tgttccagag cttcttccag
     8521 tccgcgccgg cgctggccga ccgccggggc accggtgtcg ggctcaacgt cgcccgtcgc
     8581 tacgcgcagc tccaggccgg cgggctcgac ctcgagagcg agctcggcgt cgggaccacg
     8641 ttcaccttca cgctccccgc cgccgactga gcgtcgcctt cggcgacctc acgtcgcgtc
     8701 gggcccggcg atgaccgtgc ggttccggcc gctgcgcttc gcctcgtaga gggcctggtc
     8761 ggccgcgccc accagcgccg gcagggaggc ggcgtgcacg ggcatcgagg ccacgcccac
     8821 ggagatggtg accgggcggg gctggccggg cgacgcgaag tggtgctcga ccgcctcccg
     8881 gagacgctca ccgagccggc aggccgagtc gaggtcggtc tcgcgcatca cgacggcgat
     8941 ctcctcaccg ccgtagcggt agacggtgtc gctgccccgg acgctcgccg acaggatccg
     9001 ggcgatggtc tgcagcgcga cgtcggccgc ctggtggccg agcgcgtcgt tgtagtcctt
     9061 gaaccggtcg acgtcgatca tggcgaaccc gagcgggcgg ccgtagcggt ggctggcacc
     9121 gacctcccgg tccaggtcgg tctccatccg ccggcggttc ggcagctgcg tcagcgcgtc
     9181 cgtcatcgcc atcgtctcgg tgtgctcgtg cacgcgcgcc gcacccaccg ccgtcgccgc
     9241 ctgggtggcc agcgcctcga ggacgcgcat ggtgctgtcc ggctcctcgg catcgggcgg
     9301 gaacgccacc tcgatcacgc cgatcacctc ggcgccgacc accatcggca ccgccacggt
     9361 cccgccggcc tcggggtcgt cgtccagtgc ccggccgacc gagcccttcg gctggccttc
     9421 gatgcggccg aaccgggcgg cgcgaccgat cgatccctcg ccgatcggga cgggctccag
     9481 tccgacgggc tcgagcgcgg gccccgagct gtccgcgatc gcctcgaggg tctggtcgtc
     9541 cgtgctgcgc agccacacga ccacgcggtc ggagtcggtg atgttcgtcg tcgcggagca
     9601 caggccgcgg atgacgtagc gcaggttcag gctgccggcg acctcccgtg cgaagcgcag
     9661 gacctggccg agctggcggt tctgcgcccg cagctggtcc ccctgcacgt cgacggccgc
     9721 acgggctgat gcgagctgcg ccgccgtctc gtcgatgccg cgctggatgt cagccagctc
     9781 cgacggcccc gacggcgccg gcccgggctc gaggtccccg gcagccaccc gatcgaggcg
     9841 cagctgcacc gcgtccaggg cacccttgac gactgcgcgg agccggcgac cctgcacgag
     9901 cgtgagggcg aggctggcga ccgccagcgc gacggcgagg gcggtcgtgc gggtcaccgc
     9961 ctcggtctgg ccgtcgaggg cgtcctcgcg gcggtcctgc agcgcgccga cgagcgcgac
    10021 gtactcgctg cggtagtcgt cgaagagctc cttgccgtcg agcagcagag cctcgccggt
    10081 ctcggcgccg tcgcccccgc ccgccccgag ctccagggcg tcggggaccc accgatcggt
    10141 ccacgccgcc tgcgccagcc ggagatcgag gtggcgctgg cggatgtcgt cgtcggccgg
    10201 cagctgctcc gcgccggtgc ggtcgagcac ctcctgggca ttccggaacg cttcgaggaa
    10261 accgtcgtcg ccggtgagcg cgtaggcgcg cagcccggcg tgctggtcga gcatggcctc
    10321 gtgcgcggtg cggacgatcc gggtcgcctc gatgctgtcc cgcacgcgag gcccgtggtg
    10381 ctggttgtgc gcgacggcga gcgcgaggag ggcgatcgcc acgatggcga ggcccacggt
    10441 cgtgaccagc tgccaccgct ccatgcgggt cagcagcccg gtggccggct gggcttcgcc
    10501 ctgttgtgct tccgctcgtc gcacgtggtc cccagaccgg ttcgctttgc ttcgccgcgg
    10561 cgaacatacc ggacgttcgt aggatcggcc cggaacccga ccgaggaggc cccgtgcccg
    10621 acgacgtcac cgcctacgac cctcagaccg cgctcgtcgt ggtcgacgtg cagaacgact
    10681 tcgccgatcc cggcggatcg ctgtacgtgc gcgggggcca ggagatcatc ccggccgtca
    10741 acgacgagat cgccgccgcc cgcgccggcg gggccaccgt cgtcaccacc caggactggc
    10801 acccgccgac cacgccgcac ttcaccaccg acggcggcac gtggcccgtc cactgcgtcc
    10861 gggccacctg gggtgccgag ctgcatcccg agctcgacgt cgaggccgac ctcgtcgtgc
    10921 ggaagggcac cggcggggag gacggctact cggccttcac cgtgctcgac ccggacaccg
    10981 gcgacgcacg gccgaccggg ctggccggct acctgcgcga gcggggggtg cgccgcgccg
    11041 tggtggtcgg cctggctgcc gacgtgtgcg tgaaggccac cgccctcgac gccgaggcca
    11101 tcgggctcga cacgacggtg cgctgggacg ccacccgggc ggtcgacgtg gagcccggcg
    11161 acggcgaccg ggcgctggcc gagcttcgcg acgcaggcgt gcgcatcgtc ggtgccgaac
    11221 cgtgaccgac gccggtccac ctcgctggca ccgcagcgcg ctgttcaccg actcctacca
    11281 gctggtcatg gcccagctct acgtcgagga cggcatcgcc gaccgcaccg cgcagttcga
    11341 ccacctctac cggtccaacc cggactacgg cagccaccag gccggcttct gcgtgttcgc
    11401 cggcctcgag cccatgctcg agtggatggc cacggtggcg gtcaccgacg ccgacctgac
    11461 cgcgctcgcg gcgcagcgca acccggccgg ggaccggcgc tactccgacg acttcctcgg
    11521 ctggctgtcc gagcacggcc acttccgcga cctggaggtg caggccgtcg ccgagggccg
    11581 ggtggtccac ccgaacgtgc cgatgatgac cgtcaccggc ccgttcgccg ccgcccagct
    11641 gctggagacg gccctgctca accagtgcaa ctaccccacg ctgatcgcca ccaaggcgac
    11701 gcggatcgtg gagagcgccc ggggcggtga cgtgctggag ttcggcatgc gccggggccc
    11761 gggagccgcc gtggacgagg ccgcccgggc cgccctcatc ggcgggtgct cggccacgtc
    11821 gaacgtgcag gcgtcggtcg ccctcggcac cgaccccaag ggcacccacg cccacagcct
    11881 cgtccaggcg cacctggcgc tcggcagcgg cgagctgggc gccttccggt ccttcgcccg
    11941 ccgctacccc gacgaatgca tcctgctggt cgacaccgtg gacacgctgc gctccggggt
    12001 gcccaacgcc atccaggtct tcgaggagct ccgggccgcc ggccacgagc cggccggcat
    12061 ccggctcgac tccggcgacc tcgcccacct ggccgtggag tcggcccgga tgctcgacga
    12121 cgccggcttc gagcgggccc gcatcgtgct gtccggtgac ctcgacgagg tcaccatctg
    12181 gcaggtgcgg acccaggtga tcgacgaggc gccgaaggcc ggcctggacc ccgacgccct
    12241 ctgtcgccgg ttggtctacg gcgtgggcac ccgactcatc acctccgagg gggacccggc
    12301 gctcggcggc gtctacaagc tcaccgccat caccgacgag accggcgcgt ggctcccggc
    12361 tgtgaagctg tcggagaacc cggccaaggt gccgatcacc ggacccaagg cgtgctggcg
    12421 gctccacgat cgtcggggcc gggccaccgt cgacctcatg accgcccccg acgaggtgcc
    12481 cttcgacggg gtcgaccgga tgaccgtgca ccaccccgcg cagtccgggg tgcggcggga
    12541 gatccaccgc gacgacatca cccgggtgga gcccctgcac gacgtcgtgt tcgccgacgg
    12601 ccgcgccacc cgaccgccgt cgacgctgga cgagctccgg gcccgtcgcc gggcggacgt
    12661 cgacgacctc gatcccgggg tccggcgcat ggtcaacccg caccgctacc acgtctcgct
    12721 cagcgaccgg ctgcaccgga tccagcagga gacggtggag cggctccgcg ccggacgctg
    12781 agcgaccgcg atccggaggg ggacttcgcc ggcgggacgg cgaatgatcc cccgtgaccc
    12841 gaaccagccg cctcgccgcc gccgtcgtcg ccgtcctcgt gctcttcgtg ccggagagct
    12901 ccgccgagga gagcgacgcc gtccactccc ccaacctcgt gcccgtggcc aacctggcct
    12961 acgaggaccg gtacggcacc ggcgccaacc agggcaccga catcgagttc gcccggctga
    13021 agcacaaggg caaggtccgg gacttcgccc tggccggctc ctacgacaac ggtctccaga
    13081 tcatcgacgt ctccgatccg tcggccccga ccgtcgtggg cgcctacgac tgcggcatct
    13141 cccagggcga cgtgcaggtc ttcacccgcg gcaagcggac gctggcgacc tacacgatgg
    13201 acgccgggta caccctccag gaggagtcgg cttgcgtccg cgaggccaag gcgctcggcc
    13261 tgttcgagcc gaccacgtcc cacccgctcg acatcgaccc gatcggcgcc atcgcccccg
    13321 attcggcgta cggccgggcc ggcgtcggca cctacatcgt cgacatcacg gacccgaccc
    13381 gaccgacgac ggtctccttc gtcggcgtgc cgaagggctc gcacaaccag accgtgcacc
    13441 cgagcggcga ctacctctac aactccaaca gccagctgta caccacggcg ctgaacgccg
    13501 gcatcgaggt gtacgacatc cgggacctgg gcgctccccg cctcgccgcc gttctcccgc
    13561 tgccgcccgt cccgggcctc ggcagcgagt cccacgacat cacgttcaac gagcagggga
    13621 cgcgggcgta ctccgccgcc ctgtcccaca cggcgatcat cgacaccacg gtggtcgacc
    13681 gccccgagat catctcgacc atcgtcgacc cgaccatcaa cgtccaccac cagtcgacga
    13741 aggtgacgct caccgacgcg aacctcgggg tcacccggga cttcctgctc atcgaggacg
    13801 agctggccgg cgccgccggc ttcgacccgt gcccctcggg cggcctccac atctacgacg
    13861 tgaccggccc cctcgaggcc gctcccgtga agctcggcta ctggaacatc gccgacctgc
    13921 ggtaccccac ggccgagggc aacgtgggcg cccagtgcac catgcacgtc ttccaggtct
    13981 tccccgagga gggcatcctc acggccgcct tctacaacgg cggcgtgcgg gtcgtggaca
    14041 tctccagcct ggtcggcgtg gccctcgggg ccaccggcgt gggcatgcag gagatcgccc
    14101 acgcccggtt ccccgactcc ctcacctggg cggccaagac gccccgcatc gaccccgacg
    14161 gcgcgttcca cctgttcggc aacgacctga accgcgggct cgacgtctac cgcttcgatc
    14221 cgaccgccga gcggtccgac gggctcagca cgtggctcgc cgccggcgag gtgccgatcc
    14281 ccgaggccac gaccgccagc tccgacgaca cgctcttctg cctcctgggg cgcaacgcga
    14341 gctgagcccg ccccgctaca caggggccat gcatgcagtc ggtgtgaaca gcttcggtgg
    14401 tccagatgcc ctggagatgg tggagctgcc cgacccggtt gccggtccgg gccagctcct
    14461 ggtccgggtg cacgccgcca cggtgaaccc caccgacacc tacgtccgca acggcgcccg
    14521 cgccgagcac cagcgcaagg acccgccgcc gtacgtgccc ggcatggacg tcgccggcgt
    14581 gctcgaagcc atcggcgacg gcgcggagac cgacctgcag gtcggcgacc gcgtcatggc
    14641 cgtcgtgctg cccgacggcg cccacggcgg gtactgccag cgcctggcgc tgccggccgg
    14701 ctcggtcgcc cgcatgccgg cgggggcgtc gttcgccgag gcctccacca tcccgatgaa
    14761 cgggctcacc gcccggctcg cgctcgacga gctcgacgtc gcctcggggg gcacggtcgc
    14821 ggtcaccggc gccgccggcg ccttcggcgg ctactgcgtc cagctggcca aggcggacgg
    14881 actccgggtg atcgccgacg cgtccgaggc cgacgagcag ctggtgcgcg acctcggcgc
    14941 cgacatcgtc gtccgccggg gcgacggggt ggccgagcgg atcctcgagc acgcccccga
    15001 cggcgtcgac ggcctctgcg acggctcggt ccaggacgag gccctcttcc ccgccatccg
    15061 cgacggtggc ggcttcgctg cggtgcgcgg cttgaaggtc gaggccccgc gcggcatcca
    15121 catgcaccgg gtgtgggtca gccggtacct ggaggagcag gcggagctcg accggttgcg
    15181 ccagcaggtc gaggacggcc aggtcgccat gcgggtcgcg cagacgttcc cgatggacca
    15241 ggccgccgag gcgcaccgcc tcctggaggc cggcggcatc cggggccgcc tcgtcatcga
    15301 gctgtagacc ccgagacgcg gagctgggcc gcgggacacg cactccgtgc gtgcaccgcg
    15361 gcccagaacc aggggggctc cagcgggtgg ggctcagccg gcggacggcg ggagcatcac
    15421 cgagtcgatc aggtgcacgg tggcgttggc ggtctggatg tcggccacga cgacgttggc
    15481 ctggccacca ccgacggaga cggtctcacc gtcggcgctg atctccaggg tctcgcccgt
    15541 gaaggtctcc agctcggtca cgcccgccag atcggcagcg gacagctgct cgccggcgat
    15601 gacgtggaag ccgaggacct gggtgagcag gccgctgggg tcggcgaggg cggcgtcgag
    15661 ggtcgccggg tcgagtgcgg cgaaggcgca gtcggtgggg gcgaagaccg tgagggcctc
    15721 ggcggagttg agggtgtcga cgagctcggc ctcggtgacg gcggtgacca gcgtggtgag
    15781 cacggggttg ctggaggctg cggtgccgac cgggtcgtcg gtcatgccgg cgagcgtgcc
    15841 ctcttcgggg ccaccctcga ccgcggcgac gatggcgtcg gcgtcgcagg cctcggcgga
    15901 gtcggccggg gcggcgctct cgtcgcccat gtcgtcgccc atgtcctcgg acatctcgtc
    15961 ggtggtctct tcgaccgggg cgtcggcgga ggtgtcggtg tcgtcgtcgt cgccacacgc
    16021 ggcggcgacg agggagaggg acagcgcgag gccggccact cggatcaagc ggaagcggtt
    16081 catctcaggg gtactcctgt ggtggtgggt tggggggttt cgtccgcccg gtcgacccgg
    16141 gcggcgatct ggggcgagcc gctcatcacg gctcgcggac ggtgacgagg agcgactgcc
    16201 agcccgacgc gccgtcgggg aggggttcgt cgcgctcctc gcgctggagc tggccgtcgc
    16261 cgtcggtgca gcggacggcg aggcggtggg cgccggccgt ggcggtccag ggcatgcgcc
    16321 actggcgcca cgtgtcgtcg gtgagagcgt cggcgagctc cgcctcggcg aaggggccgt
    16381 cgtcgatgct gacctcgacc ttgctgatcc cccgggtctg ggcccaggcg acgccggcga
    16441 tgacggtgtc gcccgcctcg acgatctggc ccgagcgggg gacgtcgatg cgggacatcg
    16501 tcttgatggg tgcccgctcg gcgtagccgc ggggcaccca gtactgggtg aaggtgtcga
    16561 accgggtgag ctcgatctcc gccagccact tcgtggccga cacgtagccg tagaggccgg
    16621 gggtgacgag ccgggcgggg aagccgtgct gcaggggcag gggctcgccg ttcatgccga
    16681 cggcgacgag ggcgtcacgg tcgaacgcgg cctcgacggg gaagccgcag gtgtagccgt
    16741 cgaccgagcg gccgacgatc tggtcggccc ggtcctggac accggcccgc tcgagcagca
    16801 ggcgcagggg gacgcccagc cagcgggcgt tgccgacgag gtcgccgccg aggcggttcg
    16861 acacgcaggt gagcgtgatg tcggcctcca ccagcccgag cccgaccagc tcgtcgaagc
    16921 cgatctcgaa gggctcgtcc accatgccgt gcacccgcaa ggtccagtcc tcgaccggga
    16981 cctgggggat cagcagcgca gtgtcgatgc ggtagaagcg gtcgttcggc gtgaacagcg
    17041 gggccaggcc gtcgatgccg tcgaacgatg cgccggccgg tgccgacggg agcggttggc
    17101 gggctcccgg cagcaccagc gcggcgcgcg acgcggccgc gctgaagcgg tcgcgcagcg
    17161 cccgcccgcc ggcggccatg accaggccga gcgcggtgag cgctccggcc ccggcgagca
    17221 tctcacgccg cccgacccct gcatcggacg ggatgcgacg ctcgccggag cgcggacgta
    17281 gggcgaccag catcgtgctg gcgccgatca gtgcggcgag gaccgccggg acggcagcgg
    17341 ccgcggggcc gccgtcgatc gaggcggcga ccccgaccac gccgaagagg gcgaagccgg
    17401 cgacccccgc ccaagggcgg cggcgggccg ccacgccgag gagcggggcg acgaccgcga
    17461 ggatgagcag gatcccgatg atcagggcgg ccttgtcgtt ggtcccgaag gttcggatcc
    17521 cgaagcgggt gagggcggag ggcgagcggt cgatcaccga ttcggcgacg gcgaccaccg
    17581 gcgagcgcca cgtctgcacg acgccggcgg ccagctcggc tgcgccgagc gcagcgacgg
    17641 cggcagcgct cgcccagagg gcggcgggca gcgtcggggt gcggggtgcg ttctccatct
    17701 cacgtgcact acgaggccgg gcacggatcc ggatgacgcg aacggatcag tcgggctcgt
    17761 gctccgcccg ccaggagcgg aaccgggcga ggtgaccgct cggtgccgga gccaggatcg
    17821 gcggctcgcc ggcgtgcacc cgcccggtcg tgccgtcgat ggtgacgatg tcgccgtccc
    17881 ggaggacctg ccctccggct cggacggtgc ccgcggcctc atccacctcc agcgcctcga
    17941 cgccgaccac cgccggcagg tcgagctccc gggcgacgag cgcggcgtgg ctgacgaggc
    18001 cgccccgtgc ggtgagcacc ccggcgctgg ccaccatgcc gcggatgtcg tcgggggagg
    18061 tctcgcggcg tacgagcacg acggcgccgt cgtggctcga cacgtgctcg ggcgacaggc
    18121 acacctcgcc cgtggccacc ccggggcagg cgccgaggcc ggtggccaac acgtcggctc
    18181 cgtccgggtc gacggtcggc tgcgaggcgt ggcggagctg gtcctggtcg acccgggcca
    18241 gcgcctcggc gacgctgatc acgccctcct cgacgaggtc gacggcgatc cgcacggcgg
    18301 cgggcgccgc ccgggcgccc acccggctct ggaggaggtg gagggtgccg tcctcgatcg
    18361 tgaactcgac gtcgaccatg tccccggtcg ccgcctcgat cgcggccacg gcggcccgga
    18421 gctcgccgtc gacccggggc cagagccgtc cgaggtcgtc gatcggccgg gggcgatgcg
    18481 acccggcgac gacgtcctcc ccctgcgcgt cgaccaggag gtcgccgcac agcgccgggg
    18541 cgccggtgct cggatcccgg ctcgacgcga cgccggcacc cccgcccgcc ccgcggttgc
    18601 cgaacaccat cgcctgcacc acgacggcgg ttccgccggc gtcgtcgaga ccgtgccgct
    18661 cgcggtaggc ccgagcccgc tcgttctccc aggaccggaa caccgccacg atggcgtcga
    18721 ggagctggtc gtgggggtcg tcgggcaccg cgccggcgag cgccgccgcc agctcgtcgg
    18781 gatcgtcgcc ccgctgcggg gtggcgccga gcacgatcga tgcccagccg gtcaggaagc
    18841 gggcatggca gctgcgggcg aaggcgtcac ccgcccggtc ggccagggcg gtgcgggtgt
    18901 ggggcgcgaa cccgacgtcg aggacggtgt ccatcatccc cggcatggag atcgccgcac
    18961 ccgagcggac ggataccagc agggggtcgt caccgccgaa gcggcgcccg gtcgcctcct
    19021 cgaggtcggc gaccgcggcc cggaccgcgt cgatcgtggc tcggggcacc tgcccgtccg
    19081 ccagcacccg acggcagacg tcggtggtca ccacgaacgc cggcggcacc cggaggccca
    19141 ggtcgatcat gcgagccagg ctcgccgcct tggcgccgac gagcgagggg tccaggtccc
    19201 gggcctgggt gacgggcacc acgccggcgg tcccggcggt gcggtcgtgg tccacggcgc
    19261 cattctcccg cgcggcggcc gccgtccgcc gccgtgcgag gctggatgcc ggtgctcccg
    19321 atcttcgccg ccgacgcgct gctgcccgcc cgctaccaga tggcggtcag cctcggctgg
    19381 cacatcctca tcgccgcctt cggcgtggcc ttcccggcga tcatcttcgt cgcccaccga
    19441 cgaggtctcc gcggggatcc ggccgggctg gtcctcgccc agcggtgggc gaaggtcagc
    19501 ggcgtgctct tcgccctcgg cgcggtcagc ggcacgatcc tgtccttcga gctgggcctg
    19561 ctgtggccgg ggatgatggg gacgttcggc gacgtcatcg gtccggcgtt cgcgtgggag
    19621 ggcctcgcgt tcttcaccga ggccatcttc ctcggcatct acctgtacgg cttcaaccgg
    19681 ctggcgcccc gcacgcacct cctcaccctc gtgccggtgg tgcttgccgg catcctcggg
    19741 agcttctcgg tgatcgccgc caacgcgtgg atgaactcgc cggccgggtt cgacatcgtg
    19801 gacggcacgg tgaccgacat cgacccggtc gccgccatct tcaacgaggc cgtgtggctg
    19861 cagtggatcc acatgctgtt cgccgcgtac atggtggtcg gcttcacggt ggccggcgtc
    19921 tacgcgctcg gccgcctccg ggggcgcgac gacggcgccc atcgcctcgg cttcatcgtc
    19981 ccgttcgcgt tcgcctcggt cgccacgctg ctgcagcccg tcgtcggcca cctcgcgggc
    20041 gagcggctcg ccgaggcgca gccgtcgaag ttcgccgcca tcgagctgct gccggagacg
    20101 acggacggcg cgcccctcac catcggcggg gtgctcatcg acggcgaggt ccgctacggc
    20161 atccagatcc cgaacctggg gtcgctcatc gccaccggct cccccgatgc ccgcatcgtc
    20221 ggcctcgacg acgtgcccga gggcgccgag gtgcccgcct cgatcgtgca cctgtccttc
    20281 cagctgatgg tcgggatcgg cttctcgctc gtcgcgctgg tgctctggta ctggttccgc
    20341 cggtggcgag ggtcggaccc gctcgaccgc agcacctggt tccgccgggg cgtcgtcgtc
    20401 gccggccccg cggcgatgac cgccctcatc gccggctgga tcgtcaccga ggtcggccgg
    20461 caaccctgga tcgtgtaccg cgtgatgcgg gtggacgagg cggtcaccga cgccggctgg
    20521 atctggtgga gcctcaccac catcgtcgtg gtctacgcgt cgatgaccgt gttcggggcg
    20581 gtcgtgctgc gggccatgag ccgccggtgg cgcgagggcg gcgagatgcg ggtgccgtac
    20641 ggacccgaca ccgaggacga cgaggcgggg gtcgacgtgt ccgccggcgt cccgggcccg
    20701 gggggcgacg gctcgtgagc gccgacctcg tggccgccgc catgttcgcc ggggtcgtcc
    20761 tctacgccct cttcggaggc gccgactacg gcgccggcgt ctgggacctc accgccggtc
    20821 ggggcgacat ggccgaccgg gcccgccgcc agatcgatcg ctcgatcggg ccggtgtggg
    20881 aggccaacca cgtctggctc atcttcgtca tcgtcatgct gtggaccggg ttcccccggg
    20941 cgttcagcgc gatcatgacc acgctcgccg tgccgtggtc cttcgccggg ctcggcatcg
    21001 tgttccgggg cggtgcgttc gtcttccgca agtcctcccc cacctacgcc caggcccagc
    21061 tgcacggcgt cgtcttcgcc gcctcgtcgg tcgtgacccc cttcttcctc ggcaccatcg
    21121 ccggtgccat cgccgccggg cgggtgccga tcgacggggg cggcgacccg ctcacctcgt
    21181 ggacgggtcc gctgtccatc gtcggcgggg tgctggccgt gctggtcacg gcgttcctcg
    21241 ccgcggtcct cctcaccgtc gatgccgacc gtcgcggcga tcacgagctc tcggagtggt
    21301 tccgccgtcg cgccctgctc gccgccgccg tcaccggggt ggccgcgctg accgcagcgg
    21361 ccacgatcga ggcgacggca ccgtcgctcg ccgacggcct ccacggcccg ggcgcacccc
    21421 tggtcgtcgc ctcggccgtc gggggggtca cgaccgcgtg ggcgctgctc cggcggcggc
    21481 tggccgtggc ccggatcggc gcgttcgtgg ccgtcgccgc cgtcgtcacc gggtggggcg
    21541 tcgcccagta ccccgacgtg ctcgtcgacg tcgccaccat cgacgacgtc gccggcgcgc
    21601 ccgtcaccat gcgcatgctg ctggtggtgg tcgcggccgc aggcgtgatc gtggtgccgg
    21661 cgctggtgtg gctgctgcgc ctcgtcgacc agccgcgctg gaagtagccc ggctcagcga
    21721 ccccgcttgg cgaggtacct cttgtagttc tcggccgact cgcgcagctc gggccgggca
    21781 tcgagcacgg gctggatctt ctcccacgcc tcttccttct tgtacgggat gaactcccgg
    21841 gggaagtagc cctcgtgggg gcggtagtcc ttgagcaccc ggcgggcgac cgtggacttg
    21901 tgcacctcgt cggcgccgtc ggccaggccc atggtggggg cgccggcgta catggcctgc
    21961 agcggcgtca ggtcggtggt gccgagggag ccgtggatgt gcagggcgtc gaaggagatc
    22021 tcccgcagca cccgcgccat cgtgaacttc accgcggcga tgtcggtgcg ggtctcggca
    22081 ctggacgtct ggtccatctt ccacgccgtc tcgagcacgt agagccgcag catcttgagc
    22141 ttggcgtagg actcggcgat cttctcctgc accatctggt gctcaccgat gatcttgccg
    22201 tgcgactccc gcgacagcgc ccgctcgcac atcatgtcga aggccagcgt gcactgggcg
    22261 atggtgcgca tggcgtggtg gatgcgaccg ccgccgagcc ggcgctgggc caggaccttg
    22321 gcgccgtcct cggggccgag gaggtggtcg gccgggacgc gcacgtcgtt gtagatgatg
    22381 tggttgtgat tgcggggctg gggctggatc tccacgcccg gggtctcgcg gggcaccacg
    22441 aacatgccgt tggtgcacat gacgaagagg atgtcggcga cgcgtccggc ggacgtgaac
    22501 cacttctccc cgttgatgac ccactcgtcg ccgtcgcgga ccgccgtggt gcggaacagg
    22561 ttcgggtcgg agccgccctg gggctcggtc atcgagtagg cggagaacat ctcctggttg
    22621 agcagcggct cgagccagcg cttcttctgc tcctcggtgc cgtaggcggc gagcatctcc
    22681 atgttcccgg tgtcgggcgc ggcggcaccg aacatctgcg gggcgccgga gtagcgaccg
    22741 atcacctcgt tgagcaggcc gagcttgagc tggccgaagc cggggccacc cagctcctcg
    22801 tcgaggaaga tcgcccacag cccctggtcc ttgacctcct gctgcagctc gcgcacgtag
    22861 gccttcacca ccgggtcggg tgaccggacc gcgtagggga agacgtagtc gagcggctcg
    22921 accttctcct tgcagaagtt ctcgacccag tcgagcttct cctggaattc gggttcggtc
    22981 tggaagtccc atgccatggc ccgcagtgga tcaatgcagg tgcattgatg tcaagccggg
    23041 tcgcgtgcgg gtcagcgcag gagcctgcgg gtgtcctccg acaccggtgc cagcaggagc
    23101 ccggtgacca ggtcgacgag ctggctggtg aagaggcggt ggtccttgcg gccggtgcga
    23161 ccgcgccggg cgagctcgga caccgtgaag cgcagcgcgt cgaaccgccg gtggagccgg
    23221 atcgcctcgg gggtcagcat cggcatcacc agggcccgcc agcggttgac gctgtaggtc
    23281 ggatcctcgg cggcggcggg ctcgtagggc gggttcggtc ggttgaagag gtccgacagg
    23341 agctgcaggt agccggggcc gccgtcctcg ttgcccagct cgtcggccag cggctggacc
    23401 agggcgtggg ccaggcgccg gatgtctgcg gtgccggcct cctcgtaggc gtcgagcagc
    23461 gcgtgccgac gtcgctcgac gtcgacgtcg tgcttggcga gcacggcccg gatcagtcct
    23521 cgccggtcac cgaagtggta ctgcaccgcg ctggcgttgg tcgcccccgc ctcggccgtg
    23581 atctcccgca ggctcacctg gtccgacccg cgctcggcga agagccgctc ggcagctcgg
    23641 atcagcgcat cggggacggg gctggccatc ggccaggtgt ctaaccgacc gttctgggcc
    23701 gctccacacg ccgccggggc gcgtgccgcg accccgttcg tcgcctcggc tcgttcagac
    23761 gaggcggaag cgcaccccgc agatcgtccg tccgtcgccg gcgcgggcgc ggtcgctggc
    23821 gtgcaagtcg acggcgacca ggccgcgctc ccccgtcgcc caccgcagct cgacgccgtc
    23881 gtcgaagtgc ttccggccca gcagctcgcg ccagcggcgg gccgtggcgt ccggctcggg
    23941 aacctcgagc tccacggcgg tgatgccgtc gacgacgtcg gtgcggaccg cctgctgcca
    24001 ggtcggtccg gccggcatcc acgggtcatc ctcgcccgcg ggctggacgt cgatctcgag
    24061 gaacgagcca ccggtgtcgc ccgggtggag ctgccagccc tggtagccgt cgtcgtcgaa
    24121 ctcgaaggcc gtgcggacgc cgagctcctc ggcccgctgc cggaacgggg cctggtcgtc
    24181 ggtctggcag atcaccatgt agccgccgtc gccgcccatg cgctcgagct ggcggccggc
    24241 ggcggtgccg tcccgcgtgg gcgagacgac ctcgaggaac tgcctgccga cgggcagcac
    24301 ggcgttcacg agaccgaacg tggccacggc gggatcgcgg tgcgcgacct ccagcccgag
    24361 ctcggtgccg agcgcctcca ggacggggtc gagctcggtg gcgaccaggg cgacctggcg
    24421 cagtcggagc gtgctcatcg tctcgggcac gtcagcctcc caggcgcggg aacgacggcg
    24481 cccgcttctc gatgagggcc gcagcaccct cccgggcgtc aggacggtcg agggcggcgt
    24541 actgcacgcc gtcgatctcg tcgagcgcgt ccgggtacgt caccccgtag ccctcgtaga
    24601 cgaggcgctt cgtgtcggcc agcgaggccg gcgagacgtt ctcggccagg tccacgacgt
    24661 agcgccgggc tgcgtcgagg aggtcgtcgg gggccacgac ctgctgcacc agtccgaggc
    24721 gcagcgcctc ggccgcctcg accttccgcg acgaccagag catgtcgagc gcggcgccgg
    24781 t
//
LOCUS       DATISU010000342        13001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_117986, whole genome shotgun sequence.
ACCESSION   DATISU010000342 DATISU010000000
VERSION     DATISU010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 13001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13001
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117986"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VLR27_13620"
     CDS             <1..209
                     /locus_tag="VLR27_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04543.1"
                     /translation="RPAVVRFALDGSLVVGGFLLGGQLGAGTVAMTVLVPALIEPALR
                     VGHRLADTPMPAALARPPRIAELT"
     gene            267..2210
                     /locus_tag="VLR27_13625"
     CDS             267..2210
                     /locus_tag="VLR27_13625"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSP04544.1"
                     /translation="MPDALRLPFDEPASFFVVVFLAILVGPILARWVRLPAIVGLVLV
                     GVAIGSGGAGLLQRDGTIELFGTAGLLYLMFLAGLDLDRESFARERKGALVFSLSTTV
                     LPLVVTTLAGMALGLEFLAAALLASAFTSHTLVTYPTVQRFGLAETRPAVISLGGTLV
                     STIVALLVLAVVAAAHSGTLGPVFWLSFVGGIVLFFAAAFTGLPRLAQWFFTGLGTDR
                     TVRYSFTLAIAFGAALLAEVLGIEPIVGAFVAGLALNRFVPGDALLMERIQFLGSSLL
                     IPIFLVSTGMLIDPVELITDPEGLVLGAVLTVAAVGSKVLAVYPTRAVTKLSRSEIGL
                     MVGLTTAQAAGALAAAIVALDIGLIGVTEVNAVVLVILLTCVLSGALTERFAQSIPRP
                     DRPTPKIGKTVVVPIADSNRGQQLVEVAAAIARRDSGVVVPLTVLNFDAAPDEVERLR
                     DELTSEVESVALGSGADLRSMVRIDVTASAGMLHTAIESGASSILLGWKGYPARSSSY
                     FGEDTDAIITLSPVPVLVCRPADLAPSRVVLDVIDRDFSPVARPTTQLCVEVGERIAR
                     HFGVPFVVLTDQHDDLVRQVVHEEVELVTDERDSLQSLPDRTVPGDIVIGGMPPTRAG
                     LDRRASQLARALPGRTLLMVSGR"
     gene            2212..3015
                     /locus_tag="VLR27_13630"
     CDS             2212..3015
                     /locus_tag="VLR27_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HSP04545.1"
                     /translation="MRTVRVDDPDDPRLVELTQLTDATARSRIEAEHGCFVAEGILTL
                     EAVLASNHPLRAVLVAEPKLDRVAALLGHTDVDVFVGPPPLREGVTGSPIHRGVIASV
                     GRLPLPEPEALLAGARRVLVIEAVNDHENLGSLFRNAAAFGVDAVLLDPRCADPLYRR
                     SVRVSLGHVLRVPWTRIGPLAESLTLLGDAGLRTMALTPAGERTVHDLAVGPDDRLAW
                     LVGAEGPGLDAATLAAADERVRIPIAPGVDSLNVATAAAVALALSSTLA"
     gene            complement(2996..4204)
                     /locus_tag="VLR27_13635"
     CDS             complement(2996..4204)
                     /locus_tag="VLR27_13635"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP04546.1"
                     /translation="MTAPPSSDAPAGPAADDERLLTPLFALLIAAGLCYFLSIAALLP
                     TLPLYVEEELGGGGLEVGIVVGAFAVSAALIRPTVGRLGDVVGRRTMSMVGAGLAAIA
                     VVPLGLVPAIWFLVVLRLVAGLGEACFFIGAATAAQDLAPDHRRGEAASLFSVAIYSG
                     LAFGPTVGEAVYKAWGPTSTWLFAGGSAAIAFVLSWFIPREMGRAENPPPRRGLLHPA
                     AILPGSVLLMGILGFTGFAAFLPLYVDEVGTDQAGPFFLVYGVIVLFVRIVGARVPDR
                     LGPVRTSSYALVSIAAGVGVVAAVGSVTGIWIGTVLLAIGMSLLFPALFTLAVNGAPA
                     EERSHAVGTFSLFFDLSQGLGAPALGLAVTAFGTERAAFVVGALVALGGLVLANTRLR
                     AAAPVTRASS"
     gene            complement(4224..5015)
                     /gene="nudC"
                     /locus_tag="VLR27_13640"
     CDS             complement(4224..5015)
                     /gene="nudC"
                     /locus_tag="VLR27_13640"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HSP04547.1"
                     /translation="MAERPFTPLVDPPTATSADAPLVFTVRGDQVHLAEVDTVDPDAL
                     GEGHLYLGQLGGSDCWAVDVDGDEEPDVLLTPLMALHANVSEAEWLVAGRAVQLVQWR
                     RTHRFCGSCGGPTEMVPGERAMRCEDCRLMAFPRLAPAVITLVTRPDGAALLARNAAW
                     PMPMFSCLAGFVEPGETLEHAVAREVEEEVGIEIGKVTYQGSQPWPFPHSLMIGFHAE
                     YESGDIEVDGVEIAEADWFTTDDLPPIPPRLSIARRLIDEWIAAS"
     gene            complement(5008..5919)
                     /locus_tag="VLR27_13645"
     CDS             complement(5008..5919)
                     /locus_tag="VLR27_13645"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSP04548.1"
                     /translation="MPAPSPSETDRPPAPRGDLALLGVAVLAVSTSGPLIRYAAAPAL
                     AIAMWRNALALPVLAIPAWRARRPTRREARLIGLSGVFLAAHFATWVPSLSYTTVASS
                     VALVATQPVWAALIARARGEHVHRQVWIGIGLALVGVLVLSGVDLSISPRALFGDLLA
                     LVGGALAAAYVSVGSEVRRSVGTAVYTTGSYGVASALLLLACLVSGRPLAGFSTATWL
                     AIAALVVGPQLLGHTLVNTVLRSITATAVSVAILFEVVGATVIAALWFGETPPLAAVP
                     AGLLIFSGIIVVIRAGRRRPVEGAPLG"
     gene            complement(5925..6824)
                     /locus_tag="VLR27_13650"
     CDS             complement(5925..6824)
                     /locus_tag="VLR27_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HSP04549.1"
                     /translation="MDDQTAPADGYDEITYEVDGAVATLTLDRPDRLNAFTGRMMQEM
                     IAAFDEIDRDDRVRAVIVTGAGRGFCAGADLASGGDTFDSDAQSGGSAPSEMEQRDGG
                     GLVTLRIFECTKPVIAAINGPAVGIGATMTLPMDVRLASTQSKFGFVFARRGIIPEAC
                     SSWFLPRLVGISQAAEWCYSGRVFGPEEALAGGLVRSIHEPDELLGAAQELADEIAQN
                     TSAVSVAMTRAMLWRMLGESHPMAAHEVDSPGIAYLGKSADAREGVTSFLEKRPPAFT
                     DTVSEDMPPFYPWWEERGFRDGR"
     gene            complement(7047..7763)
                     /locus_tag="VLR27_13655"
     CDS             complement(7047..7763)
                     /locus_tag="VLR27_13655"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSP04550.1"
                     /translation="MTAVAVVAVLVAAALGAFVQGSVGFGHNLIAAPVFALVDERLVP
                     GPAIASAGVLVLLTVVRDRRGLHLGEVGTALIGRVPGTILAAATVAILPADDLAVVFA
                     VLVLAAVAITASGVSVRPTRSTLVGAGALSGFMGTATSIGGPPMAMLYAGEQGRRLRG
                     TLAGFFLTGILMSLLALVVTGSFGMEELRLSALPVPAIVVGFALSSWGARRLDAGRTR
                     PAVLAVAVLSAVSVLGDALR"
     gene            complement(7774..9678)
                     /locus_tag="VLR27_13660"
     CDS             complement(7774..9678)
                     /locus_tag="VLR27_13660"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP04551.1"
                     /translation="MGGFGPPAGSPFGQPGATGLPFAGVPEELQKRVEELLDGEPEHE
                     VDEVEFSQSDWDSRPFTLRRFVGERRWAMIGAVVLVVLETAMVSAGPFLTGEAIDRGI
                     VPRDTDMLLLIAGLYVGSVVLGTILSAARVAWTGRVGESLLESLRVRIFTHFQRLSLN
                     FFTGEKAGVLMTRMTSDIDALTALFQEGLVNMFVQGLTLAIVAVVLFVVSPTLAAVLF
                     LGVVPVMLVLTIWFRKASDVAFLDVRERIADVMSDLQENLGGIRVVTATNRAKRNVVS
                     HDNVLGRHRDANDRTAVIGAVYGPSTELLGYLAQAMLLGLGGWLVSEGRLSIGELAAF
                     FLYLTMFFAPIQQLVQLYNTYQQGRSAVRKLADLFATEPEVIEAPDAHPLPDMQGAID
                     LEHVTFGYDPAVPVLRDLDLHIRAGETVAFVGETGAGKSTVAKLVNRYYDPVEGTVRI
                     DGHDLRDVTLDSVRSQIGVVPQEPFLFAGSIKDNLTFAAPGATDDQLWEAVDAVGLRD
                     LVERQPEGLDQIIHERGSSLSSGERQLLALARAFLSQPRVLVLDEATSNLDLQSETRI
                     ERALDAVLEGRTAIIIAHRLATAMRADRIAVIHDGELVELGSHDELVARGGRYAALFD
                     TWMSQASAST"
     gene            complement(9678..11570)
                     /locus_tag="VLR27_13665"
     CDS             complement(9678..11570)
                     /locus_tag="VLR27_13665"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP04552.1"
                     /translation="MSLTENRTGQDESTAPAPPPGLDVDAPATRYEPLPPTAEYHPPR
                     GHIHPDTSLGWVRRMWPVVWAEKKRFGIALGMAFIALLANVAIPAVVGEGIDAIRAQT
                     DVRTWVVLLLVLSVARAFFTYGYRSRLYKFAYSIEYDLRSLMFRHLATMSHGFYDKVQ
                     TGQLVSRANSDIRAVQMLLAFAPFMAMMSMTFVLAFAYMLTKHVLLTLVAISTLPLVY
                     VFGVKLRDIMFPLSWVVQGRLAEVATTVEENVTGVRVVKAFAGEQRQIQQLARQARRL
                     KWAAVKVTDSRARYAPLMENLPRLGMALTLLYGGYLAIDGKVTVGTIITFSSYVLLLQ
                     APFRMLGFFMIMSQRAAASAGRIFEILDTPAEIVDDPHADDLVDPAGAVTFDHVTFRY
                     GEDDQLVLEDFDLEVRPGETMALVGGTGSGKSTVARLLLRFYDVDEGAVRIDGHDVRD
                     LTMASIRHHVGMVTDEPFLFSSSIHDNIAYARPDATRDEVVAAAKAANAHHFIGELAD
                     GYDTVIGERGYTLSGGQRQRIAIARTLVANPAVLVLDDATSAVDVRVEAEIHEALEHL
                     SADRTTIVIAHRLSTIALADRVALLQHGKVLAVGTHQELLVHEPHYREVLATADLVDD
                     DEEVDG"
     gene            complement(11567..11998)
                     /locus_tag="VLR27_13670"
     CDS             complement(11567..11998)
                     /locus_tag="VLR27_13670"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSP04553.1"
                     /translation="MPKNDAGVRAVARLGRVFEKRLADEGMSLPQFRVLAFLSEGEWA
                     ASKVAEWLAVSRPSLTSLVDGLVEQGWVERREDPTDRRSVLHFLTDAGRTRLAEATEE
                     LSEGLDTLLDHLDDDERARAEDGLALLHTALHRHHEAMVTS"
     gene            complement(12041..12934)
                     /locus_tag="VLR27_13675"
     CDS             complement(12041..12934)
                     /locus_tag="VLR27_13675"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HSP04554.1"
                     /translation="MALKLDYMVRETGTNLFRNLSLTIASILTVAVSLSLVGGSVLLR
                     YSVDNATQQWQGGIEFIVFMQPDATEAQSDAVERQLTESTQVERFEYFDQERTFEEFK
                     DLFQNTPQIVESVTPDILPPSFRVVPVDKSAETIESLRSVFVTQPGVREVVAAFDSIR
                     TIRNLSNLIGVGLMVVAGFLLLAATLLILNSIRMAMFARRREIEVMKLVGASNWFIRI
                     PFMLEGVFQGLIGSGFAIGVVFGVQRVFDNIAADQRFSLFSGLVVDNQQVMTTSVFVM
                     VTGVLIGALGSAFAVSRFLDV"
BASE COUNT         1947 a         4537 c         4810 g         1707 t
ORIGIN      
        1 tccggcccgc cgtcgtccgc ttcgccctcg acggcagcct ggtcgtcggg gggttcctcc
       61 tgggtggcca gctcggggcc gggaccgtgg ccatgaccgt cctggtcccg gcgctcatcg
      121 agcccgccct gcgggtcggc caccggctgg cggacacgcc catgcccgcc gccctcgccc
      181 gcccaccacg gatcgccgag ctgacctgac ctgagccgac gcgacggcgt ccggaccggg
      241 ccgcgccgcc tggctagcgt cgccggatgc ccgacgccct gcgactgccg ttcgacgagc
      301 cggcgtcctt cttcgtcgtc gtcttcctgg ccatcctcgt cgggccgatc ctggcccgct
      361 gggtgcgctt gcccgccatc gtcggcctcg tcctggtcgg ggtcgccatc ggctccggcg
      421 gtgccgggct cctgcagcgg gacgggacca tcgagctctt cgggaccgct gggctcctct
      481 acctgatgtt cctcgccgga ctggacctcg accgcgagag cttcgcccgg gagcgcaagg
      541 gcgcattggt cttctcgctc tcgaccaccg tgctccccct ggtcgtgacc accctggccg
      601 ggatggcgct cggcctcgag ttcctcgccg cggcgctcct cgcctcggcg ttcacgtcgc
      661 acacgctcgt cacctacccc acggtgcagc gcttcggcct ggccgagacc cggcccgccg
      721 tcatctccct cggcggcacc ctggtctcca cgatcgtggc cctgctcgtg ctcgccgtgg
      781 tcgccgcggc gcacagcggg acgctcggcc cggtgttctg gctcagcttc gtcggcggca
      841 tcgtgctgtt cttcgccgct gcgttcacgg gtctgccccg cctggcccag tggttcttca
      901 ccggcctcgg caccgaccgc acggtccggt actcgttcac cctcgccatc gcgttcgggg
      961 ccgccctgct ggccgaggtg ctcggcatcg agccgatcgt cggggccttc gtcgccggcc
     1021 tcgcgctcaa ccggttcgtc cccggcgacg cgctgctcat ggagcggatc cagttcctcg
     1081 ggagcagcct gctgatcccg atcttcctcg tgtccaccgg gatgctcatc gacccggtgg
     1141 agctgatcac ggaccccgag ggcctcgtcc tcggggccgt gctcacggtg gcggcggtgg
     1201 gctccaaggt gctggccgtg taccccaccc gagcggtgac gaagctctcc cgcagcgaga
     1261 tcgggttgat ggtcggcctc accaccgcgc aggccgccgg cgcgctggcc gcggccatcg
     1321 tcgccctcga catcggcctc atcggcgtca ccgaggtcaa cgccgtggtg ctggtgatcc
     1381 tgctcacgtg cgtgctctcg ggcgccctca ccgagcgctt cgcccaatcg atcccccggc
     1441 ccgaccgtcc gacgccgaag atcggaaaga cggtcgtggt gccgatcgcc gacagcaacc
     1501 gcggccagca gctcgtcgag gtggccgccg ccatcgcccg tcgggactcc ggcgtcgtgg
     1561 tccccctcac cgtcttgaac ttcgacgccg cacccgacga ggtggagcgg ctgcgcgacg
     1621 agctcaccag cgaggtcgag tccgtggcac tcggcagcgg cgctgacctg cgctcgatgg
     1681 tccgcatcga cgtgaccgcg agcgccggca tgctccacac ggcgatcgag agcggcgcga
     1741 gcagcatcct gctcggctgg aagggctacc ccgcccggag cagcagctac ttcggggagg
     1801 acaccgacgc catcatcacc ctttcgcccg tccccgtgct ggtgtgccgg ccggccgacc
     1861 tcgcgccctc acgggtcgtg ctcgacgtca tcgaccggga cttcagcccg gtggcacgtc
     1921 ccaccacgca gctgtgcgtg gaggtcggtg agcgcatcgc ccggcacttc ggggtcccct
     1981 tcgtcgtgct caccgaccag cacgacgatc tggtccgcca ggtggtgcac gaggaggtcg
     2041 agctcgtcac cgacgagcgc gactccctcc agtcgctgcc cgaccgcacc gtgcccggtg
     2101 acatcgtcat cggcgggatg ccgcccaccc gagccgggtt ggaccggcga gcatcccagc
     2161 tcgcccgggc gctgcccggc cggaccctgc tcatggtgtc ggggcgctga cgtgcgcacg
     2221 gtgcgggtcg acgatcccga cgacccgcgc ctggtcgagc tgacccagct caccgacgcc
     2281 accgcccgct cccggatcga ggccgagcac ggctgcttcg tcgccgaggg catcttgacc
     2341 ctggaggcgg tgctggcctc gaaccacccg ctgcgcgccg tgctggtcgc cgagcccaag
     2401 ctcgaccggg tcgccgccct gctcggccac accgacgtcg acgtcttcgt cgggccgccg
     2461 ccgctgcgcg agggcgtcac cggctccccg atccaccggg gggtcatcgc gtcggtcggc
     2521 cggctccccc tcccggagcc ggaggccctg ctcgccgggg cacgccgggt gctggtgatc
     2581 gaggcggtca acgaccacga gaacctcggc tccctcttcc gcaacgccgc agcgttcggc
     2641 gtcgacgccg tgctcctcga cccccgctgc gccgaccccc tctaccggcg ttcggtgcgg
     2701 gtgtccctcg gccacgtgct gcgcgtgccc tggacccgga tcgggccgct ggcggagtcg
     2761 ctgacgctgc tcggggacgc cggtctgcgc accatggccc tcacgcccgc cggcgagcgc
     2821 accgtccacg acctcgccgt cggccccgac gaccgcctcg cctggctggt gggcgccgag
     2881 ggccccgggt tggacgccgc caccctcgcc gcggccgacg agcgggtgcg catcccgatc
     2941 gcgcccggcg tcgactccct gaacgtggcc acggcggcgg ccgtggccct cgccctcagc
     3001 tcgacgctcg cgtgaccggt gcggctgccc gcaggcgcgt gttggccagg acgagcccac
     3061 cgagcgccac gagcgcgccc accacgaagg cggcccgctc ggtgccgaac gcggtcacgg
     3121 cgaggccgag cgccggcgcc ccgagcccct gggacaggtc gaagaacaac gagaaggtgc
     3181 ccacggcgtg gctccgctcc tcggccgggg ccccgttgac ggcgagggtg aacagggccg
     3241 ggaacagcag cgacatgccg atggcgagca ggaccgtgcc gatccagatg ccggtcaccg
     3301 agcccacggc ggcgacgaca ccgacgccgg cggcgatgga caccagggcg taggacgacg
     3361 tgcgcaccgg gccgagccgg tccgggaccc gggcgccgac gatgcgcacg aacagcacga
     3421 tcacgccgta gacgaggaag aacgggccgg cctggtcggt gccgacctcg tcgacgtaca
     3481 gcggcaggaa cgcggcgaag ccggtgaacc cgaggatgcc catgagcagc accgatccgg
     3541 ggaggatcgc cgcggggtgg agcaggcccc ggcggggcgg cgggttctcg gcgcggccca
     3601 tctcgcgagg gatgaaccac gacagcacga acgcgatggc ggcgctgccg ccggcgaaga
     3661 gccaggtcga ggtcggcccc cacgccttgt agaccgcctc ccccaccgtc ggcccgaagg
     3721 ccaggccgga gtagatggcg accgagaaca gcgacgcggc ctcgccccgc cggtggtcgg
     3781 gggccaggtc ctgggcggcc gtggccgcgc cgatgaagaa gcacgcctcc cccagcccgg
     3841 cgacgaggcg gagcaccacc aggaaccaga tcgccgggac gaggccgagc ggcaccacgg
     3901 cgatcgctgc gaggccggcg ccgaccatcg acatggtgcg ccgtccgacc acgtcgccga
     3961 ggcgaccgac cgtcgggcgg atgagggctg ccgacacggc gaacgcgccg accacgatgc
     4021 cgacctcgag accgccgccg cccagctcct cctcgacgta cagcggcagc gtgggcagca
     4081 gggcggcgat ggacaggaag tagcagagcc cggcggcgat gagcagggcg aacagcggcg
     4141 tgagcagccg ctcgtcgtcg gcggcaggac cggcgggcgc gtcggaggag gggggcgcgg
     4201 tcatggcagc gatcggggcg ggttcagctc gcggcgatcc actcgtcgat cagccgacgg
     4261 gcgatcgaca ggcgaggcgg gatcggcggg aggtcgtcgg tggtgaacca gtcggcctcg
     4321 gcgatctcga cgccgtcgac ctcgatgtca ccggactcgt actcggcgtg gaagccgatc
     4381 atgagcgagt gcgggaacgg ccacggctgg gagccctggt aggtgacctt gccgatctcg
     4441 atgccgacct cctcctcgac ctcgcgggcc acggcgtgct ccagggtctc ccccggctcg
     4501 acgaagccgg cgaggcagga gaacatcggc atcggccagg ccgcgttgcg ggccaggagg
     4561 gcggcgccgt cggggcgggt gacgagggtg atgaccgccg gcgcgaggcg ggggaaggcc
     4621 atgagccggc agtcctcgca ccgcatcgcc cgctcgccgg gcaccatctc ggtcggtccg
     4681 ccgcagctcc cgcagaagcg gtgcgtccgg cgccactgga cgagctgcac ggcccgtccc
     4741 gccaccagcc actccgcctc cgagacgttg gcgtggagcg ccatgagcgg ggtgaggagc
     4801 acgtcgggct cttcgtcgcc gtcgacgtcg accgcccagc agtcgctgcc gccgagctgg
     4861 ccgaggtaca ggtggccctc gccgagcgcg tcggggtcga ccgtgtccac ctccgcgagg
     4921 tggacctggt cgccgcgcac cgtgaacacg agcggtgcgt ccgccgatgt cgcggtgggc
     4981 ggatcgacga gcggggtgaa gggacgttca gccaagggga gctccttcga cgggtcgacg
     5041 gcgtccggcg cggatcacca cgatgatgcc tgagaagatc aggagtccag cggggaccgc
     5101 ggcgagcggc ggggtctccc cgaaccagag ggcggcgatc accgtggcgc ccaccacctc
     5161 gaagaggatg gcgaccgaca cggcggtggc cgtgatcgag cgcaggaccg tgttgaccag
     5221 ggtgtggccg agcagctgcg gacccacgac cagcgcggcg atggcgagcc aggtggcggt
     5281 gctgaacccg gcgaggggac gtcccgacac gaggcaggcg aggagcagga gcgcggacgc
     5341 gaccccgtag gagccggtgg tgtagacggc ggtgcccacc gagcgacgga cctcggagcc
     5401 cacggacacg taggcggcgg cgagggcgcc gccgaccagg gccagcaggt cgccgaacag
     5461 cgcccggggg gagatcgaca gatcgacccc cgacaggacc agcaccccga cgagcgcgag
     5521 cccgatgccg atccacacct gccggtgcac gtgctcaccc cgggcgcggg cgatgagagc
     5581 ggcccagacc ggctgggtgg ccaccagggc gacggaggag gccacggtgg tgtacgagag
     5641 cgagggcacc cacgtggcga agtgggcggc caggaacacc ccggacagcc cgatcaggcg
     5701 cgcctcgcgc cgggtcggcc ggcgggcccg ccacgctggg atcgccagga ccgggagggc
     5761 gagcgcgttg cgccacatgg cgatcgccag cgccggggcg gcggcgtagc ggatgagggg
     5821 accggacgtg gagacggcga gcaccgccac gccgagcagg gcgaggtcgc cgcggggtgc
     5881 gggcgggcgg tccgtctccg acggggaggg ggcgggcaag cgggtcagcg cccgtcgcgg
     5941 aagccccgct cctcccacca cgggtagaac ggcggcatgt cctcgctgac ggtgtcggtg
     6001 aacgccgggg gccgcttctc caggaaggac gtgacgccct cgcgggcgtc ggcgctcttg
     6061 cccaggtagg cgatgccggg cgagtcgacc tcgtgggccg ccatgggatg ggactccccg
     6121 agcatccgcc acagcatcgc ccgggtcatg gccacgctga cggccgaggt gttctgggcg
     6181 atctcgtcgg ccagctcctg ggcggcgccg agcagctcgt cgggctcgtg gatcgagcgg
     6241 accagcccac cggccagggc ctcctcgggg ccgaagaccc gtccggagta gcaccactcg
     6301 gctgcctggg agatgccgac gaggcggggc aggaaccacg acgagcacgc ttccgggatg
     6361 atgccccggc gggcgaacac gaagccgaac ttcgactggg tcgaggcgag gcgcacgtcc
     6421 atcggcagcg tcatggtcgc accgatgccg acggccgggc cgttgatggc ggcgatgacc
     6481 ggcttggtgc actcgaagat gcgcagcgtg accagaccgc cgccgtcgcg ctgctccatc
     6541 tcggaggggg cgctcccgcc ggactgggcg tcggaatcga aggtgtcacc gccggatgcc
     6601 aggtcggcgc cggcgcagaa gccccggccg gcgccggtga cgatgacggc ccgcacgcgg
     6661 tcgtcgcgat cgatctcgtc gaacgcggcg atcatctcct gcatcatccg accggtgaac
     6721 gcgttcaggc ggtcgggtcg gtcgagggtg agcgtcgcga cggcaccgtc gacctcgtag
     6781 gtgatctcgt cgtagccgtc cgcgggagca gtctgatcgt ccatcggagc atcgtggcac
     6841 gcggtgcggc ggcgtcccga gggctcccag cttccggatc ctccgacctc gccgggaaag
     6901 ccgatcattc cgacaatctc cgctcaagtc ccggcgcccc cagcacgatg atgcaagtgg
     6961 cacgtcgacg gggcggttgg ccagaccctc cctctcccgc ctgtccggcc gtcccgtcgg
     7021 tgccacgacc tccccgagcg cgctcctcag cgcagggcat cgcccaggac cgagacggcg
     7081 ctcagcacgg ccacggccag cacggccggg cgggtgcgac ccgcgtcgag ccgtcgggcg
     7141 ccccacgacg acagggcgaa gccgacgacg atggcgggga cgggcagcgc cgacaggcgg
     7201 agctcctcca tcccgaagct gccggtgacc accagcgcca gcaggctcat gaggatgccg
     7261 gtcaggaaga agccggccag cgtgccgcgc agccgccggc cctgctcacc tgcgtagagc
     7321 atggccatcg gcgggccgcc gatggacgtg gccgtgccca tgaagcccga cagcgcgccg
     7381 gcgccgacga gggtggaccg ggtcgggcgg acggacacgc cggaagcggt gatcgccacc
     7441 gcggcgagca cgagcacggc gaagaccacg gcgaggtcgt cggcggggag gatggcgacg
     7501 gtcgcggcgg ccaggatcgt gccggggacc cgtccgatca gcgcggtgcc gacctcgccc
     7561 aggtgcaggc cgcggcggtc gcgcaccacg gtgagcagga ccaggacgcc ggccgacgcg
     7621 atggccgggc ccggcaccaa ccgctcgtcg acgagggcga agaccggcgc agcgatgagg
     7681 ttgtggccga agcccacgct gccctgcacg aaggcaccga gcgccgcggc gaccagcacc
     7741 gcgaccaccg ccaccgcggt catcgaggcc agatcacgtc gacgccgacg cctgggacat
     7801 ccaggtgtcg aacagtgcgg cgtagcgccc accacgggcg acgagctcgt cgtgactgcc
     7861 gagctccacc agctcgccgt cgtggatcac ggcgatgcgg tcggcccgca tggcggtggc
     7921 caaccggtgg gcgatgatga tcgccgtgcg cccctcgagg acggcgtcga gagcccgctc
     7981 gatgcgggtc tcggactgca ggtccaggtt cgacgtggcc tcgtcgagga cgaggacccg
     8041 cggctgggag aggaacgccc gggccagggc gaggagctgg cgctcgcccg aggacagcga
     8101 cgagccccgc tcgtggatga tctggtccag gccctcgggc tggcgctcca ccaggtcgcg
     8161 caggccgacg gcgtcgacgg cttcccacag ctggtcgtcg gtggcgccgg gggcagcgaa
     8221 ggtcaggttg tccttgatcg atccggcgaa gaggaacggc tcctggggta cgacgccgat
     8281 ctgggaccgc accgagtcga gggtgacgtc gcgcaggtcg tggccgtcga tgcggaccgt
     8341 gccctccacc gggtcgtagt agcggttgac cagcttggcc acggtggact tgcccgcccc
     8401 cgtctccccg acgaaggcga cggtctcgcc ggcccggatg tgcaggtcga ggtcgcgcag
     8461 caccggcacg gcggggtcgt agccgaacgt gacgtgctcg aggtcgatgg caccctgcat
     8521 gtcgggcagc gggtgggcgt cgggggcctc gatgacctcg ggctcggtgg cgaacaggtc
     8581 ggcgagcttg cgcaccgccg aacggccctg ctggtaggtg ttgtacagct gcaccagctg
     8641 ctggatcggc gcgaagaaca tcgtgaggta gaggaagaac gcggccagct cgccgatgga
     8701 caggcgcccc tcgctcacca gccagccacc gaggccgagc agcatggcct gggccaggta
     8761 gccgagcagc tcggtggagg ggccgtagac ggcgccgatc accgcggtgc ggtcgttggc
     8821 gtcgcggtgg cggccgagca cgttgtcgtg gctcacgacg ttgcgcttgg cccggttggt
     8881 ggcggtgacg acgcggatgc cgccgaggtt ctcctggagg tccgacatca cgtcggcgat
     8941 gcgctcgcgc acgtcgagga aggcgacgtc ggacgccttg cggaaccaga tggtgagcac
     9001 gagcatcacc ggcaccacgc cgaggaacag cacggcggcc agcgtggggc tcaccacgaa
     9061 caggaccacg gccacgatcg ccagggtgag gccctggacg aacatgttga ccagaccctc
     9121 ctggaacagg gcggtgaggg cgtcgatgtc gctggtcatg cgggtcatga gcacgcccgc
     9181 cttctcgccg gtgaagaagt tgagcgacag gcgctggaag tgggtgaaga tccgcacgcg
     9241 cagcgactcg agcagggact cccccacccg accggtccag gcgacccggg cggccgacag
     9301 gatcgtgccg agcacgacgg agccgacgta gagcccggcg atgagcagga gcatgtcggt
     9361 gtcgcggggc acgatgccgc ggtcgatggc ctcaccggtg aggaacgggc cggcggagac
     9421 catggcggtc tcgagcacga ccagcacgac ggcgccgatc atcgcccagc ggcgctcgcc
     9481 gacgaagcgg cgcagcgtga acggccggga gtcccagtcg gactgggaga actcgacctc
     9541 gtcgacctcg tgctcgggct cgccgtcgag cagctcctcg acgcgcttct ggagctcctc
     9601 gggcacgccg gcgaagggca ggccggtggc gccgggctgg ccgaacggcg agccggctgg
     9661 tggtccgaag ccgcccatca gccgtccacc tcctcgtcgt cgtcgaccag gtcggcggtg
     9721 gccaggacct cgcggtagtg cggttcgtgc acgagcagct cctggtgggt gcccacggcc
     9781 aggaccttgc cgtgctggag cagcgcgacc cgatcggcga gggcgatcgt ggacagccgg
     9841 tgggcgatca cgatcgtggt gcggtcggcg gagaggtgct cgagggcctc gtggatctcg
     9901 gcctccaccc ggacgtcgac ggcgctggtg gcgtcgtcga gcacgagcac ggccgggttg
     9961 gcgacgagcg tgcgggcgat ggcgatgcgc tggcgctggc cgccggacag ggtgtagccc
    10021 cgctcgccga tcaccgtgtc gtagccgtcg gccagctcgc cgatgaagtg gtgggcgttg
    10081 gcggccttcg ccgcggccac gacctcgtcc cgggtcgcgt cgggccgggc gtaggcgatg
    10141 ttgtcgtgga tggaggacga gaacaggaac ggctcgtcgg tgaccatccc cacgtggtgg
    10201 cggatggagg ccatggtcag gtcgcgcacg tcgtggccgt cgatccggac cgccccctcg
    10261 tcgacgtcgt agaagcgcag cagcaggcgg gccaccgtgg acttgcccga gccggtgccc
    10321 ccgaccaggg ccatcgtctc cccgggccgg acctcgaggt cgaagtcctc cagcacgagc
    10381 tggtcgtcct cgccgtagcg gaaggtgacg tggtcgaagg tgaccgcgcc cgccgggtcc
    10441 acgaggtcat cggcatgggg gtcgtcgacg atctcggcgg gggtgtcgag gatctcgaag
    10501 atgcgcccgg ccgaggccgc ggcccgctgg ctcatgatca tgaagaagcc gagcatgcgg
    10561 aacggcgcct ggagcagcag gacgtaggac gagaaggtga tgatggtgcc caccgtgacc
    10621 ttgccgtcga tggcgaggta gccgccgtag agcagcgtga gggccatccc gaggcggggc
    10681 aggttctcca tcaggggcgc gtaccgggct cgggagtcgg tgaccttcac ggccgcccac
    10741 ttcaggcgcc gggcctggcg ggcgagctgc tggatctggc gctgctcgcc ggcgaaggcc
    10801 ttcacgaccc gcacgccggt gacgttctcc tcgaccgtgg tggcgacctc ggcgaggcgg
    10861 ccctgcacga cccaggagag cgggaacatg atgtcgcgca gcttgacgcc gaagacgtac
    10921 accagcggca gggtcgagat ggccaccagc gtcagcagca cgtgcttggt gagcatgtag
    10981 gcgaaggcca gcacgaacgt catcgacatc atcgccatga acggggcgaa ggccagcagc
    11041 atctgcacgg cgcggatgtc ggagttggcc cgggacacca gctggccggt ctggaccttg
    11101 tcgtagaagc cgtggctcat ggtggccagg tgccggaaca tgagcgaccg caggtcgtac
    11161 tcgatgctgt aggcgaactt gtagaggcga ctgcggtagc cgtaggtgaa gaaggcccgg
    11221 gcgacggaca gcaccagcag caggaccacc caggtgcgca cgtcggtctg ggcccggatg
    11281 gcatcgatgc cctcgccgac gacggccggg atggcgacgt tggcgagcag ggcgatgaac
    11341 gccatgccga gggcgatgcc gaagcgcttc ttctccgccc acaccaccgg ccacatgcgt
    11401 cgcacccagc ccagcgaggt gtcggggtgg atgtggcccc gcggtgggtg gtactcggcc
    11461 gtcggcggca ggggctcgta gcgggtggcc ggggcgtcga cgtcgagccc gggcggcggt
    11521 gcgggggcgg tggactcgtc ctggccggtg cggttctcgg tgaggctcac gacgtcacca
    11581 tcgcctcgtg gtgccggtgc agggcggtgt gcaggagcgc caacccatcc tcggcgcggg
    11641 cgcgctcgtc gtcgtcgagg tggtcgagga gggtgtcgag gccctcgctg agctcctcgg
    11701 tggcctcggc gagccgggtc cggccggcgt cggtgaggaa gtgcagcacc gagcggcggt
    11761 cggtggggtc ctcgcggcgc tccacccacc cctgctcgac caggccgtcg accagcgacg
    11821 tcagcgacgg acgggacacc gccagccact cggcgacctt cgacgccgcc cactccccct
    11881 cggacaggaa ggccagcacc cggaactggg gcaggctcat cccctcgtcg gcgaggcgct
    11941 tctcgaacac ccggcccagg cgggcgacgg cgcgcacccc agcatcgttc ttcggcatga
    12001 atggttaggc tagcggaacc attccgccag ccaaaccgag ctacacgtcg aggaagcgac
    12061 tcacggcgaa ggcggagccg agcgcaccga tgagcacgcc ggtgaccatc acgaacacgc
    12121 tggtggtcat gacctgctgg ttgtcgacga cgaggccgga gaacaacgag aagcgctggt
    12181 cagcggcgat gttgtcgaac acccgctgca cgccgaagac gacgccgatg gcgaaccccg
    12241 agccgatgag gccctggaac acgccctcga gcatgaacgg tattcggatg aaccagttcg
    12301 aggctccgac gagcttcatc acctcgatct cccggcggcg ggcgaacatc gccatccgga
    12361 tcgagttgag gatgagcagc gtggcggcga ggagcaggaa cccggccacc accatgagcc
    12421 ccaccccgat gaggttggac aggttgcgga tggtccggat cgagtcgaag gcggccacca
    12481 cctcgcggac gccgggctgg gtgacgaaca ccgagcgcag gctctcgatg gtctcggccg
    12541 acttgtccac cggcaccacg cggaagctcg gcggcaggat gtcgggcgtc accgactcca
    12601 cgatctgggg cgtgttctgg aacaggtcct tgaactcctc gaaggtccgc tcctggtcga
    12661 agtactcgaa gcgctcgacc tgggtggact cggtgagctg gcgctcgacc gcgtcggact
    12721 gggcctcggt ggcatcgggc tgcatgaaca cgatgaactc gatgccgccc tgccactgct
    12781 gggtggcgtt gtcgaccgag tagcgcagca ggaccgagcc gcccacgagg gacagcgaga
    12841 cggcgacggt gaggatggac gcgatggtga gcgagaggtt ccggaacagg ttcgtcccgg
    12901 tctcgcggac catgtagtcg agcttgaggg ccatcagcgc tgctccgggc tcattcgtag
    12961 acgccgcggt gctggtcgcg gacgatggcg ccgcggtcga g
//
LOCUS       DATISU010000343         4814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_165207, whole genome shotgun sequence.
ACCESSION   DATISU010000343 DATISU010000000
VERSION     DATISU010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165207"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2324)
                     /locus_tag="VLR27_13680"
     CDS             complement(<1..2324)
                     /locus_tag="VLR27_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HSP04555.1"
                     /translation="MKIRATASAIPRHRYPVEPWRLVETELDHDDLGHTESLFAIGNG
                     YIGMRANPSEGREAHSHGTFLNGFHETWPIHHAEAAVAFATTGQTIVNVPDAKLMKLY
                     VDDEPLLLREADLEHYERAVDFRTGISHRELVWTTPAGKRLRVRSERMVSLEHRHLAV
                     LSFEVEMLEGSAPVVVSSQLLNRQDGEDEYHVTAAALGEGFDPRQRRTFRDRVLLPQL
                     QQHLPDANDGGEVVLGYRCAHSGMTLACAYHHEVETTCGYEADTVVGEDLAKTVFTVH
                     ADAGEKFRITKYVSYHSSRGIPSQELADRCHRTIKRARRTGVVGLREQQRAWLDDFWQ
                     RTDICVEGDEAVQQAVRWNLFQLAQASARAHEQGVAAKGVTGGGYDGHYFWDTEVYVL
                     PCLAYTNPDVARKLLRFRWKMLDAAKERAAELSQVGALYPWRTINGEEASAYYAAGTA
                     QYHINAAVAYALERYLNATADVDLLVHEGAEILVETARLWADLGFYEHNGNGRSGAVA
                     GRFRIHGVTGPDEYTTVVNDNLYTNVMARFNLRYAAWTYDFLAEWSPEDLEALQRRTG
                     VTDEEVEGWHRAAEAMYLPYDEELGIHPQDESFLDREPWDFDGTPPEKYPLLLHYHPL
                     VIYRFQILKQADVVLAMHLRGQQFSRDQKRRNFEYYDPITTGDSSLSACVQSIVAAEV
                     GHEGLAFDYFKQSLYLDLADTPGNTADGVHIANAGGVWAALIHGFSGMADTGEHLRFA
                     PRLPAAWRSMRYRLLRHGCEILVTVDPDGCTVEI"
     gene            complement(2321..3046)
                     /locus_tag="VLR27_13685"
     CDS             complement(2321..3046)
                     /locus_tag="VLR27_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HSP04556.1"
                     /translation="MVARGSTVEWQAYRAVLLDLDGVITPTASVHQVAWAELFAPWGF
                     TGDDYLTYVDGKPRYDGVRSFLTSRGVELPAGTPEDPPGDATVAAMGNRKDELFRELL
                     ERDGVAPYPGTLAVLEELDRHGIAQAVVSSSRNARPVLEAAGLGDRFEHVVDGVTIVE
                     EGITGKPAPDMFLRAAELVGVSPPDAVVVEDASSGVAAGAAGGFGLVLGVDRGGNREA
                     LLAAGADLVVEDLAETLESEGVR"
     gene            complement(3115..3354)
                     /locus_tag="VLR27_13690"
     CDS             complement(3115..3354)
                     /locus_tag="VLR27_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04557.1"
                     /translation="MSEDRPHGTDTPTGTDPDAKWSEPGYEDKSLGQAVDQDSELAEE
                     ILDEEGGDEKAAEKRFEEESHGSPARERQGKPETS"
     gene            complement(3454..3852)
                     /locus_tag="VLR27_13695"
     CDS             complement(3454..3852)
                     /locus_tag="VLR27_13695"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSP04558.1"
                     /translation="MQGIWTEELAPVVVADEESPSRLVARITVTGFGSVVAHIDVALR
                     GELCAGTTPAVAEAIDGLLPLGAVDVRFDLAELRLCTSAGIDLWVDLATRLLPHGGDV
                     RLDGAAGVVRRALDAVGVSDSGTVRRPGSA"
     gene            4043..>4814
                     /locus_tag="VLR27_13700"
     CDS             4043..>4814
                     /locus_tag="VLR27_13700"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04559.1"
                     /translation="MADTRSECMDGNEAAARVAHALSEVIAIYPITPASPMAEHCDDW
                     SAAGRPNRWGTVPDVVEMQSEAGAAGALHGALQKGALATTFTASQGLLLMVPNMFKIA
                     GELTPAVIHVAARSIATHALSIFGDHSDVMSVRSTGWAMLAAGSVQEAHDFALVSHAA
                     TLRARVPFLHFFDGFRTSHEIDKIEVLDDEVVRALVRDDDVIAHRLRGMTPEAPSLRG
                     TAQNPDTFFQAREACNPFHDAVPGIVQEVMDELGEQTGR"
BASE COUNT          658 a         1709 c         1732 g          715 t
ORIGIN      
        1 tcgatctcga ccgtgcaccc gtcgggatcg acggtcacca ggatctcgca gccgtgtcgc
       61 agcagccggt accgcatcga gcgccacgcc gccggcagcc gaggggcgaa gcggaggtgc
      121 tcgccggtgt cggccatgcc gctgaacccg tggatcaagg ccgcccacac gccgccggcg
      181 ttggcgatgt gcacgccgtc ggcggtgttg ccgggcgtgt cggccaggtc caggtagagc
      241 gactgcttga agtagtcgaa cgccagcccc tcgtggccga cctcggcggc cacgatcgac
      301 tgcacgcagg ctgacaacga cgagtcaccg gtggtgatcg ggtcgtagta ctcgaagttg
      361 cggcgcttct ggtcccggga gaactgctgg ccgcgcaggt gcatggcgag caccacgtcg
      421 gcctgcttga ggatctggaa ccggtagatc acgagcgggt ggtagtggag cagcagcggg
      481 tacttctcgg gcggggtgcc gtcgaagtcc cacggctcgc ggtcgaggaa gctctcgtcc
      541 tgggggtgga tgcccagctc ctcgtcgtag ggcaggtaca tggcctccgc ggcccggtgc
      601 cagccctcca cctcctcgtc ggtgacgccg gtgcgccgct gcagcgcctc gaggtcctcc
      661 gggctccact cggccaggaa gtcgtaggtc cacgccgcgt agcggaggtt gaagcgggcc
      721 atgacgttcg tgtacaggtt gtcgttgacg acggtggtgt actcgtcggg gccggtgacg
      781 ccgtggatcc ggaagcgccc cgccaccgcc ccggaccggc cgttgccgtt gtgctcgtag
      841 aagccgaggt cggcccacag ccgggcggtc tcgaccagga tctcggcccc ctcgtggacg
      901 aggagatcga cgtcggccgt ggcgttcagg tagcgctcca gggcgtaggc caccgccgcg
      961 ttgatgtggt actgcgcggt gccggcggcg tagtacgcgg aggcttcctc gccgttgatc
     1021 gtgcgccacg ggtagagcgc gccgacctgg ctcagctccg cggcccgctc cttggccgcg
     1081 tccagcatct tccagcggaa gcggagcagc ttccgggcga cgtcggggtt ggtgtaggcg
     1141 aggcacggca ggacgtagac ctcggtgtcc cagaagtagt gcccgtcgta gccgccgccg
     1201 gtgacgccct tggcggccac gccctgctcg tgggcccggg ccgaggcctg ggccagctgg
     1261 aacaggttcc agcgcaccgc ctgctgcacc gcctcgtcgc cctcgacgca gatgtcggtg
     1321 cgctgccaga agtcgtcgag ccacgcccgc tgctgctcgc gcaggccgac gacgccggtg
     1381 cggcgggccc gcttgatggt ccggtggcag cggtcggcca gctcctggga cgggatgccc
     1441 cgggacgagt ggtaggagac gtacttcgtg atgcggaact tctcgccggc gtcggcgtgc
     1501 acggtgaaca ccgtcttggc caggtcctcg ccgaccacgg tgtcggcctc gtagccgcac
     1561 gtcgtctcca cctcgtggtg gtaggcgcag gccagcgtca tgccgctgtg cgcacaccgg
     1621 tagccgagca ccacctcgcc cccgtcgttg gcgtcgggaa ggtgctgctg gagctggggc
     1681 agcagcaccc ggtcgcggaa cgtgcggcgc tggcgcgggt cgaagccctc ccccagcgcc
     1741 gccgcggtca cgtggtactc gtcctcgccg tcctggcggt tcagcagctg ggaggagacc
     1801 acgacgggcg ccgagccctc cagcatctcg acctcgaagc tcagcaccgc caggtggcgg
     1861 tgctccaggc tcaccatccg ctccgagcgc acccgcagcc gcttgccggc gggagtggtc
     1921 cacaccagct cccggtggga gatgccggtg cggaagtcga ccgcccgctc gtagtgctcg
     1981 aggtcggcct cgcgcagcag cagcggctcg tcgtcgacgt agagcttcat cagcttggcg
     2041 tcggggacgt tgacgatggt ctggccggtg gtggcgaagg cgacggcggc ctcggcgtgg
     2101 tggatcggcc aggtctcgtg gaacccgttc aggaacgtgc cgtgggagtg ggcctcgcgc
     2161 ccctcgctgg ggttcgcccg catcccgatg tagccgttcc cgatggcgaa cagcgactcg
     2221 gtgtgcccca ggtcgtcgtg gtccagctcg gtctccacca ggcgccacgg ctccactggg
     2281 tagcggtgcc gggggatagc gctggccgtg gcccggatct tcatcgcacg ccctccgact
     2341 cgagcgtctc ggccaggtcc tcgacgacca ggtcggcgcc ggcggcgagc agcgcctccc
     2401 ggttcccacc ccgatcgacg ccgagcacca ggccgaagcc gccggcggca ccggcggcca
     2461 cgccggacga cgcatcctcc accaccaccg catcgggtgg cgagacgccg accagctcgg
     2521 cggcgcgcag gaacatgtcc ggcgccggct tcccggtgat gccctcctcg acgatggtga
     2581 cgccgtccac gacgtgctcg aagcggtcgc cgagcccggc ggcctcgagc accggacggg
     2641 cgttgcggga cgaggacacc acggcttggg cgatgccgtg ccggtcgagc tcctcgagca
     2701 cggcgagggt gccggggtac ggggcgacgc cgtctcgttc cagcagctcg cggaacagct
     2761 cgtccttgcg gttgcccatg gcggcaaccg tggcgtcgcc cggcgggtcc tccggcgtgc
     2821 ccgccggcag ctccacgccc cgcgaggtca ggaacgaccg gacgccgtcg tagcggggct
     2881 tgccgtcgac gtaggtgagg tagtcgtcgc cggtgaagcc ccagggggcg aacagctcgg
     2941 cccacgcgac ctggtgcacg gacgcggtcg gcgtgatgac cccgtcaagg tcgagcaaga
     3001 cggcccggta tgcctgccac tcgaccgtgg acccgcgtgc gaccatggcc gccgagtgta
     3061 ggggcggacc cccacccggc cggccaggtc gctcggttca ggtcctcgcc cggttcagct
     3121 cgtctcgggc ttgccctggc gctcccgggc cggcgagccg tggctctcct cctcgaagcg
     3181 cttctcggcg gccttctcgt cgccgccctc ttcgtcgagg atctcctcag cgagctcgct
     3241 gtcctggtcg acggcctggc cgagggactt gtcctcgtag ccgggctcgc tccacttggc
     3301 gtccgggtcg gtgccggtgg gggtgtcggt accgtggggg cgatcttcgc tcatgggtcg
     3361 gtccttccgt cgtggggatg gactcgcgtc gctacccgag tcggtgcccg aacatgccgt
     3421 tccgggagat cccggaggtg cagcgcagac cgctcaggcg gacccgggac ggcgcacggt
     3481 gccggagtcg ctgaccccca ccgcgtcgag cgcccggcgc acgacgccgg cggcgccgtc
     3541 gaggcgcacg tcgcccccgt ggggcagcag acgggtggcg aggtcgaccc agaggtcgat
     3601 gccggccgag gtgcacaggc gcagctcggc gagatcgaag cggacgtcga cggcgccgag
     3661 gggcaggagc ccgtcgatgg cctcggcgac ggcgggggtc gtgcccgcgc acagctcgcc
     3721 gcgcagggcg acgtcgatgt gggcgacgac ggagccgaag cccgtgacgg tgatgcgggc
     3781 gacgagtcgc gagggggact cctcgtcggc gacgaccacc ggagcgagct cctcggtcca
     3841 gattccttgc atcacagctc caaaagaagg cccacgaaca caccggccgg ggtcacgggc
     3901 ctgggtccag ggtaccgggg accggtgtcc cttccacccc gatgatgcac ccgacggcgg
     3961 ccgtgcgctg gtgccattgg tccacagcac tggcgacctt gtcccatgcc ggatcgcgcc
     4021 ggacgacgtc accctgcgag agatggctga cacacgcagc gagtgcatgg atggcaacga
     4081 agccgcggcc cgggtcgccc atgcgctcag cgaggtgatc gccatctacc cgatcacccc
     4141 ggcgtcgccc atggcggagc actgcgacga ctggtcggcc gctgggcgac ccaatcgctg
     4201 gggcacggtg cccgacgtgg tcgagatgca gtccgaggcc ggggccgccg gcgcgctcca
     4261 cggcgccctc cagaagggcg cgctcgccac cacgttcacg gcctcccagg gcctgctgct
     4321 catggtcccg aacatgttca agatcgccgg cgagctgacc ccggcggtca tccacgtggc
     4381 cgcccgctcc atcgccaccc atgccctctc gatcttcggc gaccacagcg acgtgatgtc
     4441 ggtgcgcagc accggctggg cgatgctcgc cgccggctcc gtccaggagg ctcacgactt
     4501 cgcgctggtc tcccacgccg ccaccctccg ggcccgggtg cccttcctcc acttcttcga
     4561 cgggttccgc accagccacg agatcgacaa gatcgaggtg ctcgacgacg aggtcgtgcg
     4621 ggccctggtc cgcgacgacg acgtcatcgc ccaccggctg cggggcatga cgcccgaagc
     4681 cccgtcgctg cggggcaccg cccagaaccc cgacacgttc ttccaggccc gcgaggcctg
     4741 caacccgttc cacgacgccg tgcccgggat cgtccaggag gtgatggacg agctcggcga
     4801 gcagaccggc cgcc
//
LOCUS       DATISU010000344         2733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_630411, whole genome shotgun sequence.
ACCESSION   DATISU010000344 DATISU010000000
VERSION     DATISU010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630411"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VLR27_13705"
     CDS             complement(<1..750)
                     /locus_tag="VLR27_13705"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04560.1"
                     /translation="MRRPPPLLVALLAGLCLAAATPPWGFWIFAFVGVALLDHVLDDP
                     TLRPVQRLGRSWFVWAALLFPTLLWMRALTLPGYVIATAVFAGFLATATLLTPRGPAR
                     LLALPGAIILLEWVRWRWPFGGAPLSNLAVGQVDGPLAPILRVGGALLLVGITVTAGL
                     ALAAAWKRRWPVAAVLAAVVVLVLGLAVVAPDGEPTGERLEVALVQGGGPQGTRAATT
                     EPGIVLERHLAASADLDPDRDIDLLVWPEDEH"
     gene            complement(861..1643)
                     /locus_tag="VLR27_13710"
     CDS             complement(861..1643)
                     /locus_tag="VLR27_13710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HSP04561.1"
                     /translation="MARGTRHEHRDRFHPGRARLAVIAIVVLLAANAAVLAGVLDGSA
                     DAAVESRGDVAAAAVALAATAEPAVEADLDPLAGITLSAAPVTAEVDASAPLPVPENP
                     PANPYAPEPEIRHGTLEIPAIGLSQPLFEGVSLTAINRGPSHWPGTAMPGELGNVVVA
                     GHRTTYTRPFWDLQALQAGDELIFTMDDGSRHVYELDRISIVGPEDVHIVDQTHAHTA
                     TLFACHPRGSARQRIVGHFTLRAETQQVQITDTHQIRAPAGA"
     gene            complement(1700..>2733)
                     /gene="hemG"
                     /locus_tag="VLR27_13715"
     CDS             complement(1700..>2733)
                     /gene="hemG"
                     /locus_tag="VLR27_13715"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HSP04562.1"
                     /translation="PTDLDELAESGLLSPEGVARAAEDLDSDAPPVTDDVSVGELVRS
                     RLGDEVCDHLVEPLLGGINGGEADGLSVQACAPQIWACATRGGSLIHAAAEVKARTAP
                     SDAPVFAAPAEGMAALPRRLADHLRAEIRFATVVPPLTVDGGEIHVDDSSFSGVVIAT
                     PADVAGPLVARLDHDAAAKLEATEYASVVMVTVVARAATVDHPLDGSGVVVARDAGLS
                     ITACSFGSSKWAHWDDGRNVVFRISLGHDGDPVDWCMHSNEDLTAVALADLERLFGAP
                     VTPVGVRVGRWSRSFPQYRPGHLGRIADVRAALDAAGPIAVAGMSYDGIGVPACIRSG
                     RTAARHLLG"
BASE COUNT          370 a          932 c         1117 g          314 t
ORIGIN      
        1 gtgctcgtcc tcgggccaga ccagcaggtc gatgtcgcgg tcggggtcga ggtcggcgga
       61 tgcggccagg tgccgctcga gcacgatgcc cggctcggtg gtggcggcgc gggtgccctg
      121 cggtccgccg ccctggacca gcgcgacctc gaggcgctcg ccggtcggtt ccccgtcagg
      181 ggcgacgacg gcgagcccga ggacgagcac cacgacggcg gcgaggacgg ctgccacggg
      241 ccaccgccgc ttccacgccg ccgccagcgc gagcccggcc gtgaccgtga tgccgacgag
      301 cagcagcgcg ccgccgacgc gcaggatcgg tgcgagcggg ccgtcgacct ggccgacggc
      361 gaggttcgac agcggggcgc cgccgaaggg ccaccgccag cgcacccatt cgaggaggat
      421 gatcgcgccg ggcagggcga gcagccgggc cgggccccgc ggggtcagca acgtggcggt
      481 ggcgaggaag ccggcgaaca ccgcggtggc gatgacgtag ccgggcagcg tgagcgcccg
      541 catccacagc agggtcggga acagcagcgc cgcccacacg aaccacgatc ggccgaggcg
      601 ctggacgggc cgcagcgtgg ggtcgtcgag gacgtggtcg agcagcgcca cgccgacgaa
      661 ggcgaagatc cagaaccccc acggcggggt ggccgccgcc aggcacagcc cggcgagcag
      721 cgcgaccagc aggggtgggg gacggcgcat ggcggcggcg agtctggcac cgccccccga
      781 gcccgctgca gtcagcgcga gccgctacac gggcactgcc agttcgagag aaccggaacc
      841 ggcagtggtg gtgttgcggc tcaggccccg gccggggccc ggatctggtg cgtgtcggtg
      901 atctgcacct gctgggtctc ggcccgaagc gtgaagtgcc cgacgatgcg ctggcgggcc
      961 gagccgcggg ggtggcaggc gaacagcgtg gccgtgtggg cgtgcgtctg gtcgacgatg
     1021 tggacgtcct cgggcccgac gatcgagatg cggtcgagct cgtagacgtg gcggctgccg
     1081 tcgtccatcg tgaagatcag ctcgtcgccg gcctgcagcg cctggaggtc ccagaacggg
     1141 cgggtgtagg tcgtgcggtg gccggcgacc acgacgttgc cgagctcgcc gggcatcgcc
     1201 gtgccgggcc agtggctggg gccccggttg atggcggtga ggctgacgcc ctcgaacagc
     1261 ggctggctca gcccgatggc cgggatctcg agcgtcccgt ggcggatctc gggctcgggg
     1321 gcgtaggggt tcgccggcgg gttctccggc accggcagcg gcgcgctggc gtcgacctcg
     1381 gcggtgactg gcgcggcgct gagggtgatg ccggcgagcg gatcgaggtc ggcctcgacg
     1441 gccggctcgg cggtggcggc gagggcgacc gcggcggcgg cgacgtcgcc gcgggactcg
     1501 accgcagcgt ctgcgctgcc gtccaggacg ccggcgagga ccgcggcgtt ggcggccagg
     1561 agcaccacga tggcgatgac ggcgagacgg gcgcggccgg ggtggaaccg gtcgcggtgc
     1621 tcgtgtcggg ttccccgggc catcggtccc agtgaaccac ggatccgggc gatccggaca
     1681 ggttgacgga gatcggtcgt cagcccagga ggtgccgggc ggcggtgcgc ccgctgcgga
     1741 tgcaggccgg gacgccgatg ccgtcgtagg acatgccggc cacggcgatc ggccctgcgg
     1801 catcgagggc ggcgcgcacg tcggcgatgc gaccgaggtg gccgggccgg tactggggga
     1861 acgaacggga ccaccggccg acccggacgc cgaccggcgt caccggagcg ccgaagaggc
     1921 gctcgaggtc ggcgagcgcg acggcggtga ggtcctcgtt gctgtgcatg caccagtcga
     1981 ccgggtcgcc gtcgtggccg agcgagatcc ggaacacgac gttgcggccg tcgtcccagt
     2041 gcgcccactt ggacgagccg aaggagcagg cggtgatgga gagcccggcg tcccgggcga
     2101 ccacgacccc gctgccgtcg agggggtggt cgaccgtcgc cgcccgagcg acgaccgtga
     2161 ccatcaccac cgacgcgtac tccgtcgcct cgagcttcgc cgccgcgtcg tggtcgagcc
     2221 gggcgacgag gggaccggcc acgtcggcgg gggtcgcgat cacgacgccg gagaaggagc
     2281 tgtcgtccac gtggatctcg ccgccgtcca cggtcagcgg aggcacgacg gtggcgaagc
     2341 ggatctcggc ccgcaggtgg tcggccaggc ggcggggcag ggcagccatg ccctcggccg
     2401 gcgcggcgaa cacgggcgcg tcgctcggtg ccgtccgggc cttgacctcg gcagcggcgt
     2461 ggatcagcga gccgccccgg gtggcgcacg cccagatctg cggcgcgcac gcctggaccg
     2521 acagcccgtc ggcctcgccg ccgttgatgc caccgagcag cggttcgacg aggtggtcgc
     2581 agacctcgtc gccgaggcgg gagcgcacga gctcgcccac ggacacgtcg tcggtgaccg
     2641 gtggcgcgtc gctgtccagg tcctcggcgg cccgggccac gccctcgggc gacagcagcc
     2701 cgctctcggc cagctcgtcg aggtcggtgg gca
//
LOCUS       DATISU010000345         9647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_72132, whole genome shotgun sequence.
ACCESSION   DATISU010000345 DATISU010000000
VERSION     DATISU010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9647
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72132"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /locus_tag="VLR27_13720"
     CDS             complement(<1..1502)
                     /locus_tag="VLR27_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HSP04563.1"
                     /translation="MKPTATYRLQLHGDNTLDDARALLAHLADLGVSHVYLSPILAAV
                     PGSMHGYDVVDHASVAADLGGDEAWERFSDEARSVGLGVVVDVVPNHVSIEGGHNERW
                     LDVLEHGASSSSAEWFDIDWVGREEYQKGIVVLPVLGDLYGELLAAGELGIARDGVRF
                     RAEAPGHRFPLAPRSLPLLLHPAAARLTGDTDADPLAAESLAFVADSLDALEPATTDR
                     ARRHRAGHLDVLQDLLRRLLDQPAVAAAVDAEIDAVNADVDALHRILDEQHYRLAYWR
                     TGARELDYRRFFDITTLVAIRVERDRVFDAVHELPLRWLAEGRVDGLRIDHPDGLADP
                     GGYVHRLRHVAPDAWIVVEKILEGDEWLPEDWPVDGTTGYEAMRLVNGVLVDPTGLDA
                     LADVERRAHELCGVEAPEPEAVVLESKRYAVEELLAPELERVVDLALRVAEARPLHRD
                     HPRDDIRRAVAALAVGFDVYRTYVVPGTAGASSAVSDTDVERVDHAARDA"
     gene            complement(1499..3637)
                     /gene="glgX"
                     /locus_tag="VLR27_13725"
     CDS             complement(1499..3637)
                     /gene="glgX"
                     /locus_tag="VLR27_13725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HSP04564.1"
                     /translation="MPDETRPHPRETWPGQPFPLGASYDGLGTNFSVFSQVAESVTLC
                     LIGDDGSEERVPLTEVDAHCWHEYLPGVGPGQRYGYRVEGPWDPASGRRCNASKLLLD
                     PYAKAVTGEVEWGQEVFGYDFDDPDQRNDLDSAGKVPVGVVHSPYFDWGDDRLLNTPL
                     HETVIYETHVKGLTMTHPDVPERERGTYQGLAHPAVIDHLVGLGITAVELMPVHQFIH
                     DGHLVDKDLRNYWGYNTIGFFAPHADYSASGSTGEQVQEFKGMVRAMHEAGIEVILDV
                     VYNHTAEGNHMGPTLSFRGLDNEAYYRLVPDDRRHYFDTTGTGNSLNMAHPYSLQLIM
                     DSLRYWVTECHVDGFRFDLASTLARQFYEVDRLSAFFDLVHQDPIVSQVKLIAEPWDV
                     GEGGYQVGNFPPVWSEWNGQFRDTIRDHWRGEPHTVPELASRLTGSADLYSDETRSPV
                     ASINFVTAHDGFTLRDLVSYNEKRNEANGEDGKDGESHNRSWNSGAEGETDDPEVLEL
                     RSRQQRNLLATLLLSQGVPMLLGGDELGRTQGGNNNAYCQDNEISWYDWDEVDTDLLE
                     FTRRCIAIRREHPVLRRRRWFFGQEIRGNVDIEWYKPDGVAMEDHDWEGAPRTIGLYM
                     DGRGIRSRDTRGRPIIDDSFLLLISAEPDPIDWKLPEMDGEWVVELDTALPTGVADDV
                     RTVTADIELVPRSTVLLRFRPADDDGADER"
     gene            3841..5307
                     /locus_tag="VLR27_13730"
     CDS             3841..5307
                     /locus_tag="VLR27_13730"
                     /inference="COORDINATES: protein motif:HMM:NF027645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54 modulation/S30EA ribosomal C-terminal
                     domain-containing protein"
                     /protein_id="HSP04565.1"
                     /translation="MLATQEASPMPDGVVQWFDPSTGEGIIRRGGRRYAVRAGAMEPA
                     ARVGGARVHFDVLRDDGVLRAENVVLRHGTRVSPRQTGFGDLSGAREPAATGGTASSR
                     RNPAPVVSYDHHPARAVEDWAALLADGRLDDAVLQCAPDIVLHDGEDELAGTEALRRW
                     WDASPQLGSGATPTVAGVGPDTFELTWDQPSPNRIRVRVAHGLISEQWDDAATAAAAT
                     DEDADEGGRPAFGTLEVSSAGHVTSTELAYAEDKVAKVLTDVGRPVLHTDIRLERSSD
                     ASLERPAMARLIVLVDGEPVRAQVRASEMTEAIDLLDARARRRLTQLAEHRQTLRRRG
                     PSSPEGQWRRGDAPTERPPHLPRPAAEREIVRRKSFTTAEATVDEAIFDMEALDHDFF
                     LFTDLASGQDAVVHVLDDGSYGLQYATGTDEDPTEPTAAVVEAQTDPAPRFTVDQARE
                     HLDLSGAPWLFFVDADHDRGRVLYRRDDGHYGLIAPAG"
     gene            complement(5304..6803)
                     /locus_tag="VLR27_13735"
     CDS             complement(5304..6803)
                     /locus_tag="VLR27_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP04566.1"
                     /translation="MRRMLGRASFLVRKDLNLGNVLERLAVVHGDRRLVTEARGGLTL
                     THRQAAKRVARWAGGVAARTEPGDVVVVATPNGYQQLLLCLAVARAGCIPAPVNDAMR
                     PDEVRHVMEDSGAAFALRSAHEVDGHEPVRQAVAASGSDVAALFYTSGTTGKPKGAAL
                     THDALLGSLRGGALVPAGLREDEAVVSLPVAHIMGFVTLLGLAATGTATWFSPRFRPE
                     EVLDAIESRRPSVFIGVPAMYRLLLEAGAEDRDLGSIRVWGSGADVMPAELAQRFKKM
                     GATVCLPFVGDVGEATFAEGYGLVETGGGVAAKISPPFMRLGTGDAMGFPVPGYRLRV
                     VDEQGDDVAGGATGELLVKGPGVLRGYWHDDAATAAVLDDGGWLHTGDLARRGPLGSI
                     VFAGRKKDVIMRGGFSVYAVEVEAVLEDHPDVLEAAVVPVPDERLGEVPAAAVRLRDG
                     VELDPAALDAHAREHLAHYKVPARYVAVDDLPRTGTNKVQRSEVIALLA"
     gene            complement(6833..7918)
                     /locus_tag="VLR27_13740"
     CDS             complement(6833..7918)
                     /locus_tag="VLR27_13740"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HSP04567.1"
                     /translation="MSTGRGDGALPEHQPTRRASDDDRTQVVDRLRLAAGEGLIDLDE
                     LEQRLAAAWSARTLPELAPLTADLPATTTPPATPAQRPRRPIGMEDEGFRSHLTVYCL
                     VIGMLIGIWLLGGAGHFWPFYPAAGWGIGLGAHYQGSAAEQRKLLKKARAEGLTLNQY
                     KEREELRKAERRAAKKQERRQLSPRDGSRPALGPTPSRFVVAMFVDVVGSTSLNEALG
                     DEQWVAVRRALRDQVADCAAAHGGWEANTAGDGVLLRFHDPISAATAATELLRRLERQ
                     RAETGFAPSVRVGIHSGDAVEDGDDIIGNVVNLAARVTDAADADEILVTEHVADHLDS
                     RHTTDGRGLHTLKGISRPRHLLTLVWR"
     gene            complement(7984..>9647)
                     /locus_tag="VLR27_13745"
     CDS             complement(7984..>9647)
                     /locus_tag="VLR27_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HSP04568.1"
                     /translation="VLAHYRQRFTHVLVDEYQDTNKVQNELVVQLAKEHRNVTVVGDG
                     DQSIYGFRGADIANILQFEEAFPDVTTIPLVQNYRSTQTVLDAANAVIANNMGRKPKD
                     LWTEAGPGDKILRYHAEDEHDEAQWVTQRISAFHEAGDVRWGDIAVFYRTNAQSRVIE
                     EYLARGGIPYRVIGGTRFYDRREIKDALAYLKAVVNPTDEVSVKRVLNVPKRGVGDTS
                     VARVDAWIQGRGLSFLEGLRRADEAGVSGRALNGIARFTELLDGAVDRIDDGPGPLLE
                     HLLEGSGYLDELRNERTIESEGRLENLAELLGTASETEDVAEFLEAVSLVADTDQLDP
                     DQSEVTLMTLHSAKGLEYPIVFLVGLEDGIFPHLRTLTEPDQLEEERRLAYVGITRAM
                     HHLHITHAWSRTLFGGTQYNPPSRFLDEIPSELVEEIGGRQRASRGGRTYSGGGSERA
                     GRRAGGWSEPEGRTFGRGDRKERMEAGRDRIVENALAARDAPAPTSGAEGLGLKVGDD
                     VHHATFGDGVIIDIEGAGDKAEATIRFVDVGEKRLLLSWSPLEKR"
BASE COUNT         1278 a         3355 c         3637 g         1377 t
ORIGIN      
        1 ttggcgtcgc gggcggcgtg gtcgactcgc tccacgtcgg tgtcgctgac cgcgctggac
       61 gcaccggcgg tgccgggcac cacgtacgtg cggtagacgt cgaagccgac ggcgagggcc
      121 gccaccgcgc gtcggatgtc gtcccgcggg tggtcccggt ggagcggccg ggcctcggcg
      181 acccggaggg cgaggtcgac gacccgctcg agctccggcg ccaggagctc ctcgacggca
      241 tagcgcttcg attcgaggac gacggcctcg ggctccggcg cctcgacgcc gcacagctcg
      301 tgggcgcgac gctcgacgtc ggcgagggcg tcgaggccgg tcgggtcgac cagcacgccg
      361 ttgacgagac gcatcgcctc gtacccggtg gtgccgtcga ccggccagtc ctcgggcagc
      421 cactcgtcac cctcgaggat cttctccacg acgatccacg cgtcgggcgc gacgtggcgc
      481 aggcggtgca cgtagccgcc tggatcggcc aggccgtcgg ggtggtcgat gcgcagcccg
      541 tcgacgcgtc cctcggccag ccagcgcagc gggagctcgt gcacggcgtc gaacacgcgg
      601 tcgcgctcga cccggatggc gacgagggtc gtgatgtcga agaagcgccg gtagtccagc
      661 tcccgggccc cggtccgcca gtacgcgagc cggtagtgct gctcgtcgag gatccggtgg
      721 agggcgtcga cgtcggcgtt gacggcatcg atctcggcgt cgaccgcggc ggccacagcc
      781 ggctggtcga ggaggcggcg gagcaggtcc tgcaggacgt cgaggtgacc ggcccggtgc
      841 cggcgggcgc ggtcggtggt ggccggctcg agcgcgtcga gggagtcggc gacgaaggca
      901 agggactcgg cggcgagcgg gtccgcatcg gtgtcgccgg tcaggcgggc cgccgccggg
      961 tggagcagca gcgggaggct gcggggggcg agcgggaacc ggtggccggg cgcttcggcc
     1021 cggaaccgca cgccgtcccg ggcgatgccc agctcgccgg cggcgagcag ctcgccgtag
     1081 aggtcgccaa gcaccggcag gaccacgatg cccttctggt actcctcccg accgacccag
     1141 tcgatgtcga accactcggc ggacgaggag gacgccccgt gctcgaggac gtcgagccag
     1201 cgctcgttgt ggccgccctc gatcgacacg tggttcggca ccacgtcgac caccacgccg
     1261 agcccgacgc tccgggcctc gtcgctgaag cgctcccagg cctcgtcgcc accgaggtcg
     1321 gcggcgacgc tggcgtggtc gacgacgtcg tagccgtgca tcgagccggg cacggccgcg
     1381 aggatcggcg acaggtacac gtgggagacg ccgaggtcgg cgaggtgcgc gaggagggcg
     1441 cgtgcgtcgt cgagggtgtt gtcgccgtgg agctggagcc ggtaggtggc ggtcggcttc
     1501 aacgctcgtc ggctccgtca tcgtcggcgg ggcggaaccg cagcagcacg gtcgagcggg
     1561 gcacgagctc gatgtcggcc gtcacggtgc gcacgtcgtc cgcgacgccg gtgggcagcg
     1621 cagtgtcgag ctcgacgacc cactcgccgt ccatctcggg cagcttccag tcgatggggt
     1681 cgggctcggc gctgatcagc agcaggaacg agtcgtcgat gatcgggcgg ccgcgggtgt
     1741 cgcggctgcg gatgccccgt ccgtccatgt acagaccgat ggtgcgcggc gcgccctccc
     1801 agtcgtggtc ctccatggcg acgccgtcgg gcttgtacca ctcgatgtcg acgttgcccc
     1861 ggatctcctg gccgaagaac cagcgccggc ggcgcaggac cgggtgctcg cgccggatgg
     1921 cgatgcagcg gcgggtgaac tcgagcaggt cggtgtccac ctcgtcccag tcgtaccagc
     1981 tgatctcgtt gtcctggcag taggcgttgt tgttgccgcc ctgggtgcgg cccagctcgt
     2041 cgccgccgag cagcatcggc acgccctggg agagcagcag ggtggccagc aggttgcgct
     2101 gctggcggct ccgcagctcg agcacctcgg ggtcgtcggt ctccccctcg gcaccgctgt
     2161 tccacgaccg gttgtgggac tcgccgtcct tgccgtcctc accgttggcc tcgttgcgct
     2221 tctcgttgta ggagacgagg tcgcgcagcg tgaacccgtc gtgggcggtg acgaagttga
     2281 tgctggccac gggcgagcgg gtctcgtcgg agtagaggtc ggccgagccg gtgagccggg
     2341 aggcgagctc gggcacggtg tggggctcgc cacgccagtg gtcgcggatg gtgtcgcgga
     2401 actgcccgtt ccactcggac cacaccggcg ggaagttgcc cacctggtaa ccgccctcgc
     2461 cgacgtccca cggctcggcg atgagcttca cctgggacac gatcgggtcc tggtgcacca
     2521 ggtcgaagaa ggccgagagc cggtcgacct cgtagaactg gcgggcgagg gtggacgcga
     2581 ggtcgaaccg gaagccgtcg acgtggcact cggtgaccca gtaccgcagc gagtccatga
     2641 tcagctgcag cgagtacggg tgggccatgt tcaggctgtt gccggtgccc gtggtgtcga
     2701 agtagtggcg gcggtcgtcc ggcaccaggc ggtagtacgc ctcgttgtcg aggccccgga
     2761 acgacagcgt cgggcccatg tggttgccct cggccgtgtg gttgtagacg acgtcgagga
     2821 tcacctcgat gccggcctcg tgcatggccc gcaccatgcc cttgaactcc tggacctgct
     2881 cgccggtgga cccggacgcc gagtagtcag cgtggggggc gaagaagccg atggtgttgt
     2941 agccccagta gttgcgcagg tccttgtcga cgaggtgccc gtcgtggatg aactggtgca
     3001 ccggcatcag ctcgacggcg gtgatgccga ggccgacgag gtggtcgatg acggcggggt
     3061 gggcgagccc ctggtaggtg ccccgctcgc gctcggggac gtcggggtgg gtcatggtga
     3121 ggcccttgac gtgggtctcg tagatcaccg tctcgtgcag cggcgtgttc agcaggcggt
     3181 cgtcgcccca gtcgaagtac gggctgtgga ccacgccgac cggcaccttg ccggcgctgt
     3241 cgaggtcgtt gcgctggtcg gggtcgtcga agtcgtagcc gaagacctcc tggccccact
     3301 cgacctcacc ggtgaccgcc ttggcgtagg ggtcgagcag gagcttggac gcgttgcagc
     3361 ggcggccgct ggccgggtcc cagggtccct cgacccggta gccgtagcgc tggcccggac
     3421 cgacgcccgg gaggtactcg tgccagcagt gggcgtcgac ctcggtgagc ggcacccgct
     3481 cctcgctgcc gtcgtcgccg atcaggcaca gggtcacgga ctcggcgacc tgggagaaca
     3541 cgctgaagtt ggtgcccagg ccgtcgtagc tcgctccgag cgggaacggc tgccccggcc
     3601 aggtctcgcg tgggtggggg cgcgtctcgt cgggcacccg ggaccgatac ccagtggacg
     3661 atcgggagat acgtgacatg cggccgggag cgtggcacgg atcggggcag gtgcgtcgcg
     3721 gtcccggcgg gatcgacccc ctggcggggg acgaaggacc cttccccgac ggagggcgct
     3781 ggcgcagcct gggggcgagc ggacgcgcct ttcacccctc cctagggtga cgtcacggcc
     3841 gtgctcgcaa cccaggaggc cagtccgatg cccgacggcg tggtgcagtg gttcgacccc
     3901 tcgaccggcg aggggatcat ccgacgtggt ggacggcgct acgccgtgcg cgccggcgcg
     3961 atggagcccg cggctcgagt gggaggtgcg cgcgtgcact tcgacgtgct gcgcgacgac
     4021 ggcgtcctcc gcgccgagaa cgtcgtgctc cggcacggga cccgcgtgtc accgcgccag
     4081 accgggttcg gggacctgag cggcgctcgc gaacccgcag cgaccggtgg gacggcctca
     4141 tcgcggcgca accccgcacc cgtggtctcc tacgaccacc acccggcgcg cgcggtcgag
     4201 gactgggcgg cgctgctggc cgacgggcga ctcgacgacg ccgtgctcca gtgcgccccc
     4261 gacatcgtgc tccacgacgg cgaggacgag ctggccggca ccgaggccct ccgccgctgg
     4321 tgggacgcca gcccccagct cggctccggg gccaccccga cggtcgctgg cgtgggcccg
     4381 gacaccttcg agctcacctg ggaccagccc tcgccgaacc ggatccgggt ccgggtggcc
     4441 cacgggttga tctccgagca gtgggacgac gcagcaacgg cagcggctgc gacggatgag
     4501 gacgccgacg agggagggcg ccccgccttc gggaccctcg aggtctcctc cgccggacac
     4561 gtcacgtcga ccgagctcgc ctacgccgag gacaaggtcg ccaaggtgct caccgacgtc
     4621 gggcggccgg tgctgcacac cgacatccgc ctcgagcggt cctccgacgc gtcgctcgag
     4681 cgcccggcga tggcccgact catcgtgctg gtcgacggtg agccggtccg cgcccaggtg
     4741 cgggcgtcgg agatgaccga ggccatcgac ctcctcgacg cccgggcccg acggcgcctc
     4801 acccagctcg ccgagcaccg ccagacgctg cggcgccgcg ggccgtcgag ccccgagggg
     4861 cagtggcgcc gcggtgacgc gcccaccgag cggcctcccc acctgccccg gcccgccgcc
     4921 gagcgcgaga tcgtgcgccg caagagcttc acgacggcgg aggccaccgt cgacgaggcc
     4981 atcttcgaca tggaggcgct cgaccacgac ttcttcctgt tcaccgacct ggccagcggg
     5041 caggacgccg tcgtgcacgt gctcgacgac ggctcgtacg gcctccagta cgccaccggc
     5101 accgacgagg acccgacgga gccgaccgcc gcggtggtcg aggcccagac cgacccggcg
     5161 ccccggttca ccgtcgacca ggcccgggag cacctcgacc tgagcggcgc gccgtggctg
     5221 ttcttcgtcg acgccgacca cgatcgcggc cgcgtcctct accgccgcga cgacgggcac
     5281 tacgggctga tcgcaccggc cggctaggcg agcagggcga tgacctcgct ccgctgcacc
     5341 ttgttggtcc cggtgcgggg caggtcgtcg acggccacgt agcgcgccgg caccttgtag
     5401 tgcgccaggt gctcccgggc gtgggcatcg agcgcggccg ggtccagctc gaccccgtcc
     5461 cgcagccgga ccgccgcggc ggggacctca ccgaggcgct cgtcgggcac cgggaccacc
     5521 gcggcctcga gcacgtcggg gtggtcctcg agcacggctt cgacctcgac cgcatagacc
     5581 gagaacccgc cgcgcatgat cacgtccttc ttgcgaccgg cgaagacgat cgagccgagg
     5641 ggtccgcgcc gggcgaggtc gccggtgtgg agccagcccc cgtcgtcgag caccgcggcg
     5701 gtggccgcgt cgtcgtgcca gtacccgcgc aggacgccgg gacccttgac cagcagctcg
     5761 ccggtcgcgc cgccggcgac gtcatcgccc tgctcgtcca cgacccgcag ccggtagccg
     5821 ggcacgggga accccatggc gtcacccgtg ccgaggcgca tgaacggcgg gctgatcttg
     5881 gcggcgacgc ccccgccggt ctccaccagg ccgtagccct cggcgaaggt cgcctcgccc
     5941 acgtcgccga cgaagggcag gcacaccgtc gcccccatct tcttgaaccg ctgggccagc
     6001 tcggccggca tgacgtcggc cccggatccc cagacgcgga tgctgcccag gtcccggtcc
     6061 tcggcgccgg cctcgagcag gaggcggtac atggcgggca cgccgatgaa caccgagggc
     6121 cggcgggact cgatggcgtc gaggacctcc tcggggcgga agcgggggga gaaccacgtg
     6181 gccgtcccgg tggcggccag gccgagcagc gtgacgaagc ccatgatgtg ggccacgggg
     6241 aggctgacca cggcctcgtc ctcgcgcagc cccgccggca cgagcgcgcc gcctcgcagg
     6301 gagccgagca gcgcgtcgtg ggtgagggcg gcgcccttgg gcttgccggt cgtgcccgac
     6361 gtgtagaaga gcgcggcgac gtcggaccca ctggccgcga cggcctggcg caccggttcg
     6421 tggccgtcga cctcgtgagc cgaccgcagg gcgaaggccg cgccggagtc ctccatgacg
     6481 tggcggacct cgtcagggcg catggcgtcg ttcaccgggg ccgggatgca cccggcgcgg
     6541 gcgacggcca agcagagcag cagctgctgg tagccgttcg gggtggcgac cacgaccacg
     6601 tcgccgggtt cggtccgggc cgcgacgccg ccggcccagc gggcgacccg cttcgccgcc
     6661 tggcgatggg tgagggtcag cccgccgcgg gcctcggtga cgagacgccg gtcgccgtgg
     6721 acgacggcga ggcgctcgag cacgttgccc aggttgaggt ccttgcggac caggaaggag
     6781 gcccgcccga gcatccgacg catggagcac cggtgtaccc ccgccggggc ggtcagcgcc
     6841 agacgagcgt cagcaggtgc cgggggcggc tgatgccctt gagcgtgtgc aggccacgac
     6901 cgtcggtcgt gtggcggctg tcgaggtggt cggcgacgtg ctcggtcacg aggatctcgt
     6961 cggcgtcggc ggcatcggtg acccgggcgg cgaggttcac cacgttgccg atgatgtcgt
     7021 ccccgtcctc gacggcatcg ccgctgtgga tgccgacgcg caccgagggg gcgaagccgg
     7081 tctcggcgcg ctggcgctcg agccggcgca gcagctcggt ggcggcggtg gcggcggaga
     7141 tcggatcgtg gaagcgcagc agcacgccgt cgccggcggt gttggcctcc cagccgccgt
     7201 gggccgcggc gcagtcggcg acctggtccc gcagtgcccg gcgcaccgcc acccactgct
     7261 cgtcgccgag ggcttcgttc aggctggtgg agccgaccac gtcgacgaac atggcgacga
     7321 cgaagcggct gggcgtcggc ccgagggcag ggcggctgcc gtcgcgtggg gagagctggc
     7381 ggcgctcctg cttcttcgcc gcccgccgct ccgccttccg gagctcctcg cgctccttgt
     7441 actggttcag cgtcagcccc tcggcccgag ccttcttcag cagcttgcgc tgctccgccg
     7501 ccgacccctg gtagtgggcg ccgagcccga tgccccagcc ggcggccggg tagaagggcc
     7561 agaagtggcc ggccccgccg agcagccaga tgccgatgag catgccgatg acgaggcagt
     7621 acacggtgag gtggctccgg aacccctcgt cctccatccc gatcggacgg cggggacgtt
     7681 gcgccggcgt cgcaggaggg gtcgtggtcg ccggcaggtc agccgtgagc ggggcgagct
     7741 cgggcagggt gcgagccgac caggcggcgg cgaggcgctg ctcgagctcg tcgaggtcga
     7801 tgaggccctc gccggccgcg agccggagcc ggtcgaccac ctgggtgcgg tcgtcatcgg
     7861 aggcgcggcg cgtcggctgg tgctcgggca gtgccccgtc cccccgtccc gtgctcacaa
     7921 ggaccgattc tggcaccgag catgcatctc ctgcccggac ggctgtgacg tctggccggt
     7981 gggtcagcgc ttctcgagcg gggaccagga cagcagcagg cgcttctcgc cgacgtcgac
     8041 gaagcggatg gtggcctcgg ccttgtcgcc ggcaccctcg atgtcgatga tcacgccgtc
     8101 gccgaaggtg gcgtggtgca cgtcgtcgcc gaccttgagg ccgagcccct cggcgccgct
     8161 ggtgggtgcc ggcgcatctc gtgccgccag ggcgttctcg acgatgcggt cgcggcccgc
     8221 ctccatccgc tccttgcggt cgccccgccc gaaggtgcgc ccctcgggtt cggaccagcc
     8281 gccggcgcgg cgtccggccc gctccgagcc accgccgctg tacgtgcgcc cgccccggct
     8341 ggcccgctga cggccgccga tctcctccac cagctcggac gggatctcgt ccaggaagcg
     8401 gctgggcggg ttgtactgcg tgccgccgaa gagggtgcgg ctccaggcat gcgtgatgtg
     8461 caggtggtgc atcgcccggg tgatgcccac gtaggcgagg cgacgctcct cctcgagctg
     8521 gtcgggctcg gtgagggtgc gcaggtgggg gaagatgccg tcctccaagc ccacgaggaa
     8581 gacgatgggg tactcgaggc ccttggccga gtgcagggtc atcagggtga cctcggactg
     8641 gtcggggtcg agctggtcgg tgtcggccac gaggctgacc gcctcgagga actcggcaac
     8701 gtcctcggtc tccgatgcgg tgccgagcag ctcggccagg ttctcgaggc gcccctcgga
     8761 ctcgatggtg cgctcgttgc gcagctcgtc caggtagccg cttccctcga gcaggtgctc
     8821 gagcaggggc ccggggccgt cgtcgatgcg atcgaccgcg ccgtcgagca gctcggtgaa
     8881 ccgggcgatg ccgttgagcg cccggccgga cacaccggcc tcgtcggccc ggcgcaggcc
     8941 ctcgaggaag gacaggccac gaccctggat ccaggcatcg acccgagcca ccgaggtgtc
     9001 gcccacgccg cgcttgggca cgttgagcac ccgcttcacg ctgacctcgt cggtggggtt
     9061 caccaccgcc ttcaggtagg cgagcgcgtc cttgatctcg cgccggtcgt agaagcgggt
     9121 gccgccgatg acccggtacg ggatgccgcc gcgggccagg tactcctcga tgacccggga
     9181 ctgcgcgttg gtccggtaga agacggcgat gtcgccccag cggacgtcgc ccgcctcgtg
     9241 gaacgccgag atgcgctggg tgacccactg ggcctcgtcg tgctcgtcct cggcgtggta
     9301 gcgcaggatc ttgtcgcccg ggccggcctc ggtccacagg tccttgggct tgcgccccat
     9361 gttgttggcg atcaccgcgt tggcggcgtc gagcaccgtc tgggtggacc ggtagttctg
     9421 caccagcggg atcgtggtga cgtcggggaa cgcctcctcg aactggagga tgttggcgat
     9481 gtcggcgccc cggaagccgt agatcgactg atccccgtcg cccacgaccg tgacgttgcg
     9541 gtgctccttg gccagctgga ccacgagctc gttctggacc ttgttggtgt cctggtactc
     9601 gtcgacgagg acgtgggtga agcgctggcg gtagtgggcg agcacgt
//
LOCUS       DATISU010000346        14518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_770443, whole genome shotgun sequence.
ACCESSION   DATISU010000346 DATISU010000000
VERSION     DATISU010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 14518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14518
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770443"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VLR27_13750"
     CDS             <1..555
                     /locus_tag="VLR27_13750"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HSP04569.1"
                     /translation="EELFHADWHEAKTTLGREPLSSELARTPAQRRADALVVMAQRAH
                     VANPSAAARLRPLVVVVAGLDALAGGLAELWNETPLSARQLARILQDDPDLERYVYGP
                     GEVPIAYNERHRFFTGKLRRAIQVRDRHCTARGCDRPAEYCDIDHIDPVTQGGRTRPD
                     NGRVACNPDNRARPHRRFHGDPDP"
     gene            complement(569..1822)
                     /locus_tag="VLR27_13755"
     CDS             complement(569..1822)
                     /locus_tag="VLR27_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04570.1"
                     /translation="MAKRKRRGGGGRVTPPARVWPEDEPGLPPLEGIVAALDDFTDVG
                     DRSPLEIELQLSQFLGQLGAGSAPPGPGEPDHEELLNGIVELCLHHLEQSPPRVVLDF
                     LWVLDAFDPGWLTWPLHERLDAASVPSRPAWAMDVGQAEVRSAHRVEHETADGYDIAL
                     VARHRSADKDHVVAVYIDRTLGGLATDLLVHDDAAEYLRLSDDADGMGITDLDLAVAG
                     ATIDVAIDATLAAGVPAAVADGFSPQWAVVEHYVAKLPAGGEPLAHPDPATPEECAAI
                     VDRFLASLDGMAHKKDRQPLLDAVGFVAEELGGDPLRWSAPVTQLILGGWLPLSGFDD
                     DVAERFPRVLRAFVPWAHREHGWGDRYLPEALEVVRAAEAGELPGPSQGGGQVEILEQ
                     AVAAGVDLDDEAALDAFLDDYLDGE"
     gene            1993..2886
                     /locus_tag="VLR27_13760"
     CDS             1993..2886
                     /locus_tag="VLR27_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04571.1"
                     /translation="MPAPLGDHLATDASLHLLADHLTEALAEGELEPCLLHVATPDAD
                     GFDLGVKPLDGLHPTDALLGTTAPDDWHAVGIATHGWAYHVAERGDPGRRRHRVHVVT
                     LLSRTGEHAHRVRADDEDLEEALGEEAPGGEQVDLLRRVLGLPTDPPPCDPSTYWSIE
                     WLSALVQAEQPVETLADAIELHPVTQLLRRGTSIGEPDLVDVLTAFHRVLTWGRMREL
                     ASLGRFTVPELDPEDAAWLDDGSFARFVLNRCPPLHLLKGRLDAHCSPEVAEDVLDLL
                     EDLGVPDTTWPDLVRRPRPAA"
     gene            2932..3354
                     /locus_tag="VLR27_13765"
     CDS             2932..3354
                     /locus_tag="VLR27_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04572.1"
                     /translation="MAWFRRRTSETPASTDSAAPAPSRELVPMVSGQSLERTERALKM
                     LATQSAQLHGHVVQLEHRVDALSDALLDRMDVPSNGDLLEARMHSARVAAELSRLEVN
                     LAARLDAVRDELRAVQGQGASIDLRELTPNDTGWTPAR"
     gene            complement(3365..6376)
                     /locus_tag="VLR27_13770"
     CDS             complement(3365..6376)
                     /locus_tag="VLR27_13770"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HSP04573.1"
                     /translation="MPSPAARRPRPAGSSRGRTILIVAAIALFILVTSLRGIAGFYTD
                     FLWFDSLGYDDVWRGVLGAQISLAVIFVVIFFAICWVNLYIADRLAPKFPPAGPEQEA
                     LERFRDAIGHRTGLLRIGVALFLALIAGLGMSQEWRSWLLFVNGGDWGQEDAQFGTDL
                     GFYIFRLPFLSSVADWAFASILIILVVTVAAHFANGGIRVQPTGPGAVGPRVTSQVKA
                     HISVLLGVLALIRAAGYFLQRFELTTSSTGFVDGAGYTDVNAQLPVLNLLVLISAASF
                     VLFIVNIWRRGWVLPILGVGLWGLVAVVAGGIYPQFIQRFQVEPNESSTEEQYIERNI
                     AATREALDLGDVDSVDYELNREPEGVDLTDNRNTVRNIRLWDPSATVLGQTFPRLEEL
                     LPYYQLSDVDVDRYTIDGEPTQVVLSVRDLNTDGIPTGSWEARHVIYTHGYGVVMASG
                     TSKTPEGRPDFFLQQVPVRENTDLGLERPQIYFGEDMGGNGITGASRDELDYQIGNET
                     QLTRYDGADGVGIGSYLRRIAFSLRFGQYDPLITDFITGDSKIHYIRDVRDRAQSLAP
                     FLEFDADPYAVVVDGGVKWIIDAYTTTDRFPYGQVADNGGLPANSGLNKGFNYVRNSV
                     KTVVDAYDGTVTFYVIDDEDPIIQAWRGAFPDLFVDIDEMPDELTEHLRYPEDLFRVQ
                     TNMWARYHVNDPQTFYNNNDAWNVALDPGTAGAGPATQTTNAAGEPVGPSVGARVDPY
                     YLLTQLPGEDEAEFVMLRSFVPFGRDDDNQQLTAFMVARSDPENYGELVTYTMPRQNR
                     PDGPAIVADAMQSDPVVSEQQTLLGTGESTVKLGNLIVVPVDESLVYVRPFYVESNQV
                     RIPELKKVIAFFESNVAIADTLEQALTQLLGDVPNLDEVVDEEDREVIDVPDDPNAPL
                     DPNEQTGSVDERAARLLEEANELFEDAQAALIAGDLGEYQALTDEAQELVTEALELLA
                     ASTTTTTSPSAETEGTDPEATDPEDEESEEA"
     gene            complement(6480..8573)
                     /locus_tag="VLR27_13775"
     CDS             complement(6480..8573)
                     /locus_tag="VLR27_13775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HSP04574.1"
                     /translation="MTEDPRIDPADLRNVRFPGSSRRYDARAVDQFLETVAARIAATN
                     ELVDELQARIADMDTAAPVAAPAPPPPELSLLDEDELVKLIGSETASVLSTARRAAEE
                     IRSKAEESAARMIREATTEADRLTEEARSKATELTSDAEQALERATTEATAEAERIRS
                     EAVEEAERTTTEAAELLATEQAAADARRAEAEAEVEAILEAARDEGRGMVNEAKEVRT
                     RILEDLQRRRDIARDHIERLLDGRERLLAAYGLVRENLDDITGQLADALPDPGEPELP
                     DGFVGIVGGTVTASEDDDGEDGAADAEEQSEGEVAAVEPDDTVAPLSETEADIEGVDD
                     AVDEPEELEETDDEVQAEGSEGPAVEEPEATEPDVDDHEVTELEADEADAHAPGSGGD
                     VDELFARLRAEREESVAKARQVVEAGPDADPDPDSDSDTEPDDAADDAGTVAADEAED
                     SEPAEDAETDDAETDDAGADDRAGGDVDRVPAEFLADEEALTGRAEALGPLETKLNRA
                     LKRRLADEQNELLDLLRRSGTTTAAELLPELAIQVDGYARLAEKHLLAAAAAATQSAG
                     SEVAVDVSALAQALGAALVEPFRRRVERSASEVDGDPDELDERLRALYREWKVQHIGP
                     VADDALLSAYALGTLTAAEPDGRVSWRIDPSQGPCPDAQDNALAGEIGAGEAFPTGDA
                     CPQAHPGCRCLLVIS"
     gene            complement(8650..9789)
                     /locus_tag="VLR27_13780"
     CDS             complement(8650..9789)
                     /locus_tag="VLR27_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04575.1"
                     /translation="MSTRAVLAGCAVVVLLVGVGAVVLRDDPDDPGPDQARLQVDGAA
                     SVTAVDGATRELVDEVEVLAFGEQVAMVSGSAELELADGSTYELRSRDDTATVLEVGA
                     PPRVLAGELLAVGGFPAQVSVDTATLTAQGALRVDADDVVASAYAGRARVTGVGDVGE
                     LAGLRRLVLVAGGTPEPISFDGSDEWDRRYLGEAIAFGERLEALARGYTSDLPPGSGR
                     SLSFFQSVLPALADERELGEDLLDPARTPGETLVGAAITVQGRQGTFRERWQEVFAFR
                     AAGAAWGLVALDQGVSAAPVLDTIELAVDASDGAGMSPTTTRAGSPTATTPGVSTDTT
                     TTRPTSTTTTTTPPAPPEGGLLDPVVGPTSEVLEGLLEVLGLAPP"
     gene            complement(9786..12404)
                     /locus_tag="VLR27_13785"
     CDS             complement(9786..12404)
                     /locus_tag="VLR27_13785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP04576.1"
                     /translation="MTTEAAQLQFAAEFTLFMAAIAAIAVTALRPDLLARRALGRFLL
                     VTGAIALAAAAFLHGSLLVDDGTDPVLVGLRLGGIAAVAAAVATWRTSRGAQGAAAAG
                     VLALAAAEGLVAAELETGADVVRGLGAVLFGIGLVVASRRAIAARVAMAAAAVVLVVV
                     TVLAIALSVVIAENVEDEAAERYEARAETESQVFVDAGRSARASAELLGRVFAGQAAA
                     IQTATDPAAAPADAEAARAQIAAALAEARTSLLQLRFPGPIAVLGADGRLAAALDLSD
                     AVRVSLLGDATVAEVRETRQSSEAVDIVGNQTFAVGAAPILDADGIEFVGVAVVTTRL
                     DDEYLADRVDDGGSVDQDVGMAIADRSRIYASSGPQAGEPVIRQLAGDVLLEGERRTT
                     VDGDRFVVADTVEVDGTPKLAVVVNVPQGSIDATRQDLFRLLFLVALGAGALAMVLAG
                     YAGNRIGAGLSRLTTVTGELQQGRLSARADVGAEDELGVLASTFNQMAASLQSMTDDL
                     RSAADQESALRGRLEGVVGGMGEALVAVDDRGRITDFNASAEELVGLPARDVLGRTLA
                     KVCTITGEDGVDVTPRFVKPVVDSWTAALDLVRSDGATVPVAVSAGPLRGPAGMLTGA
                     VFVLRDVRREREIEQMKTDFIANVSHELRTPLTPVLGYADILATRELGPDQTKRFAGE
                     IHESARKLLRVTDQLVQFATLAAGRLQLHEEPVDARQLLDALADRWSDRLPDTHRLTR
                     RIGRGMPPAVLDRRYVDVALDELIDNAVKYSPEGGRIRVSASVHENGTGPRLVLAVDD
                     QGVGIDPDRRESIFEDFAQADGSMTRRFGGLGLGLALVSRIVRAHGGDLSCSSEVGKG
                     TTVRMELPLDRPADRP"
     gene            complement(12435..13571)
                     /locus_tag="VLR27_13790"
     CDS             complement(12435..13571)
                     /locus_tag="VLR27_13790"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HSP04577.1"
                     /translation="MSTEDESVPGSVSTRDHDRDIPEPPDAPVSRRRTWPWWLAAVAL
                     LGTAAALFAAFTVQIGHFEFRPGSARPTTALVDVGEGIEVHPPAGEIAFTTVSLRQST
                     VASYVWAWFDDDVQVVADELILGNRSPQENRQFNLQLMDTSKQDAIRVALLELGYEVP
                     VTIEGVAIVTVQEGSAGEGLVEVGQTVVALDGEPLDDIDDVSRIMDPKAPGDTVVLSV
                     EPPERGDATDVPVVLGASPADPEQGLIGIELQPRSPAYDFPIDVDIDSGNVGGPSAGL
                     AFTLAVLDVLTPGELTGGLDVAVTGTIDGAGNVGDVGGVRQKTAAAIDQGYDVFLVPS
                     KEFQEAEERAGDALEVIAVDTLADALDALASLGGSGLGNPADVG"
     gene            13564..14034
                     /locus_tag="VLR27_13795"
     CDS             13564..14034
                     /locus_tag="VLR27_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04578.1"
                     /translation="MLTLILLVVLGGGAGLMAVWLIDARGLSRLLGRFAPGSLRPTRP
                     ADTTRTETPEPAAPEPALTSAATVATAYEPVESSFEPLPAPEPATRRDQKKVPAAYTA
                     VEGTYREVARVPLWRKLVSLVVLLVILAVVGATIAALAGATFGLVAELVDGAVG"
     gene            14031..>14518
                     /locus_tag="VLR27_13800"
     CDS             14031..>14518
                     /locus_tag="VLR27_13800"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSP04579.1"
                     /translation="MSDDATARRRDAAVAGHIGDEATARSLLADEDHKVRATALAALD
                     RIGALGDDELAAALADPAPGVRRRALSLTATRPGDEPPAALALLDDEDPVVAETAAWA
                     AGERQPPEDGAAEALARLATEHPEHLVREAAVAAIGAVGDPVGLPAVLLALEGRATLR
                     RRA"
BASE COUNT         1917 a         5292 c         5322 g         1987 t
ORIGIN      
        1 gaagagctgt tccacgccga ctggcacgag gccaagacca cgctcggccg ggaacccttg
       61 tcgagcgagc tggcccgcac ccccgcgcag cgacgtgctg atgcgctggt ggtcatggcc
      121 caacgcgccc atgtcgcgaa cccctccgcg gcggcccggc tccgcccact tgtggtcgtc
      181 gtggccggcc tggatgccct cgctgggggt ctggccgagc tgtggaacga gacccccctg
      241 tcggcccgcc agctcgcccg gatcctgcag gacgatccgg atctggaacg ctacgtgtac
      301 gggccgggtg aggtgccgat cgcgtacaac gaacggcacc ggttcttcac cggcaagctc
      361 cgccgggcca tccaggtccg cgaccgccac tgcaccgcac gcggttgcga ccgacccgcc
      421 gagtactgcg acatcgacca catcgacccc gtcacccagg gcgggcgcac ccgccccgac
      481 aacggacgcg tcgcctgcaa ccccgacaac cgagcccgac cccaccgccg attccacggc
      541 gaccccgacc cgtaggccag gccggcggct actcgccgtc gaggtagtcg tccaggaacg
      601 cgtcgagcgc cgcctcgtcg tccaggtcca cgccggcggc caccgcctgc tcgaggatct
      661 ccacctgacc accgccctgc gagggtcccg gcagctcgcc ggcctcagcg gcgcgcacca
      721 cctccagcgc ctcgggcagg tagcggtcac cccagccgtg ctcccggtgg gcccacggga
      781 cgaacgcccg cagcaccctc gggaagcgct cggccacgtc gtcgtcgaag cccgacaacg
      841 gcagccagcc gccgaggatc aactgggtga ccggtgcgct ccagcgcagc gggtcgccgc
      901 cgagctcctc ggccacgaag cccaccgcgt ccagcaaggg ttggcgatcc ttcttgtggg
      961 ccatgccgtc gaggctggcg aggaagcggt cgacgatcgc ggcgcactcc tccggggtgg
     1021 cgggatccgg gtgcgccagc ggttcgccgc ccgccggcag cttggccacg tagtgctcga
     1081 cgacggccca ctgcggggag aacccgtcgg ccacagcggc gggcacgccg gcggcgaggg
     1141 tggcgtcgat ggccacgtcg atggtggcgc cggccaccgc caggtcgagg tccgtgatgc
     1201 ccatgccgtc ggcgtcgtcg gacaggcgca ggtactccgc ggcgtcgtcg tggacgagca
     1261 ggtcggtggc caggccgccc agggtgcggt cgatgtagac ggccacgacg tggtccttgt
     1321 ccgccgagcg gtgccgggcc accagcgcga tgtcgtagcc gtcggcggtc tcgtgctcca
     1381 cccggtgggc gctgcgaacc tcggcctgcc cgacgtccat ggcccatgcc ggccgagacg
     1441 gcaccgacgc ggcgtcgagg cgctcgtgga gcggccacgt cagccaaccg gggtcgaagg
     1501 cgtcgagcac ccagaggaag tcgaggacga cccggggcgg cgactgctcg aggtggtgca
     1561 ggcacagctc gacgatgccg ttgagcagct cctcgtgatc gggctcgccg ggtccgggcg
     1621 gcgccgaccc ggcaccgagc tggccgagga actgggagag ctgcagctcg atctcgagcg
     1681 gcgagcggtc ccccacgtcg gtgaagtcgt cgagggcggc cacgatgccc tccagcggcg
     1741 gcagtccggg ctcgtcctcg ggccacaccc gtgcgggcgg ggtcacccgt ccgccaccac
     1801 ctcgacgctt gcgcttggcc atccggcgac cctacgcgcc gaggctgtca gcccccatct
     1861 gccatgatcg gtcccgagcc gacatcggcc tcgtggatca cctccgcctc cggcgccacc
     1921 ggcgccgctc gccgccccca tcccccacgg gatgagacgg gcagccagct ccgaacgtct
     1981 ggaggctccg ccatgcccgc accgctgggt gatcacctcg cgaccgacgc gtcgctccac
     2041 ctgctcgccg accacctcac cgaggccctc gccgagggcg agctcgaacc gtgtctgctc
     2101 cacgtcgcca cgcccgacgc cgacgggttc gacctggggg tgaagccgct cgacgggctc
     2161 cacccgaccg atgccctgct gggcaccacc gcgccggacg actggcacgc cgtgggcatc
     2221 gccacccacg gatgggcgta ccacgtcgcc gagcggggcg atccgggccg gcgacggcac
     2281 cgggtgcacg tcgtcacgct gctgtcccgc accggcgagc acgcccaccg cgtccgggcc
     2341 gacgacgagg acctcgagga ggcgctcggc gaggaggcgc cgggcggcga gcaggtcgat
     2401 ctgctccgcc gggtgctcgg gctgcccacc gatcccccac cgtgcgaccc gtccacctac
     2461 tggagcatcg agtggctctc ggcgctggtc caggccgagc agcccgtcga gaccctggcc
     2521 gacgccatcg agctgcaccc ggtcacccag ctgctgcggc ggggcacgtc catcggcgag
     2581 cccgacctgg tcgacgtcct caccgcgttc caccgcgtgc tcacgtgggg ccgcatgcgg
     2641 gagctggcct ccctcggccg cttcacggtc cccgaactcg acccggagga cgcggcctgg
     2701 ctcgacgacg ggtccttcgc ccgcttcgtc ctcaaccggt gcccgccgct ccacctgctc
     2761 aagggccgcc tcgacgccca ctgctccccc gaggtggccg aggacgtgct ggacctgctc
     2821 gaggacctcg gcgtacccga caccacctgg cccgacctgg tccggcggcc ccgcccggcg
     2881 gcgtgaacgc catccaccac cgcctgcccg tggtggatct accgtcgggt catggcctgg
     2941 ttccgtcgcc gcacctccga gaccccggcg tccaccgact ccgccgcacc ggccccgagc
     3001 cgggagctgg tgccgatggt gtcgggccag tcgctcgagc gcaccgagcg cgccctcaag
     3061 atgctggcca cccagtcggc ccagctccac gggcacgtgg tccagctcga gcaccgggtc
     3121 gacgccctgt ccgacgcgct gctcgaccgc atggacgtgc cgagcaacgg cgacctgctc
     3181 gaggcccgca tgcactccgc tcgggtcgcg gccgagctgt cccgcctcga ggtcaacctc
     3241 gcggcccgcc tcgatgcggt ccgcgacgag ctgcgggccg tccagggcca gggtgcatcc
     3301 atcgacctgc gcgagctgac cccgaacgac accggctgga cgccggcgcg ctgagccgct
     3361 cccgctaggc ctcctcggac tcctcgtcct cggggtcggt ggcctccgga tccgtcccct
     3421 cggtctctgc ggacggcgag gtcgtcgtgg tcgtcgacgc ggccagcagc tcgagcgcct
     3481 cggtcaccag ctcctgggcc tcgtcggtca gggcctggta ctcgcccagg tccccggcga
     3541 tcagcgctgc ctgcgcgtcc tcgaacagct cgttggcctc ctcgagcagg cgtgcggcgc
     3601 gctcgtcgac cgatcctgtc tgctcgttgg ggtcgagggg agcgttcggg tcgtcgggca
     3661 cgtcgatgac ctcgcggtcc tcctcgtcga cgacctcgtc caggttgggc acgtcgccca
     3721 gcagctgggt cagcgcctgc tccagggtgt cggcgatggc cacgttggac tcgaagaagg
     3781 cgatgacctt cttgagctcg gggatccgga cctggttgga ctccacgtag aagggccgca
     3841 cgtacacgag cgactcgtcc accgggacca cgatcaggtt gcccagcttc accgtcgact
     3901 caccggtgcc gagcagcgtc tgctgctcgg agaccaccgg gtccgactgc atggcgtcgg
     3961 ccacgatggc cgggccgtcg ggccggttct ggcggggcat cgtgtaggtc acgagctcgc
     4021 cgtagttctc cgggtcggac cgggccacca tgaacgctgt cagctgctgg ttgtcgtcgt
     4081 cgcggccgaa gggcacgaag ctgcggagca tgacgaactc ggcctcgtcc tcaccgggca
     4141 gctgggtgag caggtagtac gggtcgacgc gggcaccgac ggacgggccg accggctcgc
     4201 cggcggcgtt cgtggtctgg gtggccggac cggcgccggc ggtgccgggg tcgagggcca
     4261 cgttccaggc gtcgttgttg ttgtagaagg tctgcggatc gttcacgtgg tagcgggccc
     4321 acatgttggt ctgcacccgg aacaggtcct cggggtagcg caggtgttcc gtgagctcgt
     4381 ctggcatctc gtcgatgtcg acgaacaggt cggggaaggc gccgcgccag gcctggatga
     4441 tcgggtcctc gtcgtcgatc acgtagaagg tgaccgtgcc gtcgtaggcg tcgaccacgg
     4501 tcttgaccga gttgcggacg tagttgaagc ccttgttcag gccgctgttc gccggcaggc
     4561 cgccgttgtc ggccacctgc ccgtagggga accggtcggt ggtggtgtag gcgtcgatga
     4621 tccacttgac cccgccgtcc acgacgacgg cgtacgggtc ggcgtcgaac tccaggaacg
     4681 gcgccaggct ctgggcccgg tcccgaacgt cgcggatgta gtggatcttg gagtcgccgg
     4741 tgatgaagtc ggtgatcagc gggtcgtact gcccgaagcg cagcgagaac gcgatgcgac
     4801 gcaggtacga gccgatgccc accccatcgg cgccgtcgta gcgggtgagc tgggtctcgt
     4861 tgccgatctg gtagtccagc tcgtcccggc tggcgccggt gatgccgttg ccacccatgt
     4921 cctcgccgaa gtagatctgc gggcgctcga ggccgaggtc ggtgttctcg cgcaccggca
     4981 cctgctggag gaagaagtcg gggcgacctt ccggggtctt cgaggttccc gatgccatga
     5041 ccacgccgta gccgtgggtg tagatgacgt ggcgggcctc ccatgacccg gtggggatgc
     5101 cgtcggtgtt caggtcgcgc accgacagga cgacctgggt gggctcaccg tcgatcgtgt
     5161 agcggtccac gtcgacgtcg ctcagctggt agtagggcag cagctcctcg aggcggggga
     5221 acgtctggcc caggaccgtg gcggaggggt cccagagtcg gatgttgcgc acggtgttgc
     5281 ggttgtcggt gagatcgacg ccctcgggct cgcggttgag ctcgtagtcg acgctgtcca
     5341 cgtcgccgag gtcgagcgcc tcccgggtgg cggcgatgtt gcgctcgatg tactgctcct
     5401 cggttgagga ctcgttcggc tcgacctgga agcgctggat gaactgcggg tagatgccgc
     5461 cggccaccac ggcgaccagg ccccagaggc cgacgccgag gatgggaagc acccagccac
     5521 ggcgccagat gttcacgatg aacagcacga agctggccgc cgagatcagg accagcaggt
     5581 tgagcacggg gagctgggcg ttgacgtcgg tgtagccggc gccgtcgacg aagccggtgc
     5641 tcgaggtggt gagctcgaag cgctggagga agtaccccgc ggcccggatg agggccagga
     5701 cgccgagcag cacggagatg tgggccttga cctgcgaggt cacccgcggc ccgaccgctc
     5761 cggggccggt gggctggacc cggatgccgc cgttggcgaa gtgggcggcg acggtgacga
     5821 cgaggatgat gaggatcgag gcgaacgccc agtcggccac cgaggacagg aacggcagcc
     5881 ggaagatgta gaagcccagg tcggtgccga actgggcgtc ctcctggccc cagtcgccgc
     5941 cgttgacgaa gagcagccac gagcgccact cctggctcat gcccaggccg gcgatcaggg
     6001 cgaggaacag cgccacgccg atgcgcagca ggcccgtgcg gtgcccgatg gcgtcccgga
     6061 agcgctccag cgcctcctgc tcgggaccgg ccggggggaa cttcggcgcc aggcggtcgg
     6121 cgatgtacag gttgacccag cagatggcga agaagatgac gacgaagatc accgcgaggg
     6181 agatctgggc gccgagcacg ccacgccaga catcgtcgta cccgagcgag tcgaaccaga
     6241 ggaagtccgt gtagaagccg gcgatgccgc gcagcgacgt gacgaggatg aacagcgcga
     6301 tggccgcgac gatcaggatc gtgcgtccac ggctcgaacc ggccgggcgc ggacggcgcg
     6361 cggcggggct tggcatgtca gagggtgggc gcatggaggg cgcggagcct accgatgacg
     6421 ggcgggccac ccatcgcgtg cccgcgccga ccggctcgat cggagcgggt gggagcgcgt
     6481 cagctgatca cgaggaggca gcggcacccg gggtgggcct gggggcaggc gtcgccggtg
     6541 gggaaggcct cgccagcacc gatctcgccg gccagggcgt tgtcctgggc gtcggggcac
     6601 ggtccctggg acgggtcgat ccgccagctg acccggccgt cgggctcggc ggcggtgagg
     6661 gtgccgagcg cgtaggcgga caggagcgca tcgtcggcca cagggccgat gtgctggacc
     6721 ttccactccc ggtacagggc gcggaggcgc tcgtcgagct cgtcggggtc gccgtcgacc
     6781 tccgaggccg agcgctcgac ccgacggcgg aacggctcca ccagggcggc gccgagggcc
     6841 tgggccagcg ccgagacgtc gacggcgacc tcgctgccgg cggactgggt ggccgctgcg
     6901 gctgcggcca gcaggtgctt ctcggcgagc cgggcgtagc cgtcgacctg gattgcgagc
     6961 tcgggcagca gctcggcagc ggtcgtggtg ccgctgcgcc gcagcaggtc gagcagctcg
     7021 ttctgctcgt cggccagccg gcgcttgagg gcccggttca gcttggtctc gagcggaccg
     7081 agggcctcgg ctcgcccggt cagggcctcc tcgtcggcga ggaactcggc cggcacccgg
     7141 tcgacgtcgc cgccggcccg gtcgtcggcc ccggcgtcgt cggtctcggc gtcgtcggtc
     7201 tcggcatcct cggccggctc gctgtcctcg gcctcgtccg cagcgaccgt gccggcgtca
     7261 tcggcggcgt cgtcgggctc ggtgtcggag tcggagtcgg ggtcggggtc agcgtcggga
     7321 ccggcctcga ccacctggcg ggccttggcg accgactcct cccgctcggc ccgcaggcgg
     7381 gcgaacagct cgtccacatc gccgccgctg ccgggcgcgt gggcgtcggc ctcgtccgcc
     7441 tcgagctcgg tgacctcgtg gtcgtcgacg tcgggctcgg tggcctcggg ctcctcgacc
     7501 gcaggcccct cggacccctc ggcctggacc tcgtcgtcgg tctcctccag ctcctcgggc
     7561 tcgtcgaccg cgtcgtcgac gccctcgatg tcggcctcgg tctccgagag cggggccacg
     7621 gtgtcgtcgg gttcgaccgc ggcgacctcg ccctcggact gctcctcggc gtccgccgct
     7681 ccgtcctccc cgtcgtcgtc ctccgacgcc gtgacggtcc caccgacgat gccgacgaac
     7741 ccgtcgggca gctcgggctc cccagggtcg ggcagcgcat cggccagctg gccggtgatg
     7801 tcgtcgaggt tctcccgcac cagcccgtag gcagcgagga ggcgctcccg gccgtcgagg
     7861 agccgctcga tgtggtcgcg ggcgatgtcc cggcggcgct ggaggtcctc gaggatccgg
     7921 gtgcggacct ccttggcctc gttcaccatg ccccggccct cgtcgcgggc tgcctccagg
     7981 atcgcctcca cctcggcctc ggcctcggca cggcgggcgt cggctgcggc ctgctcggtg
     8041 gcgaggagct cggcggcctc ggtcgtggtc cgctcggcct cctcgaccgc ctcgctgcgg
     8101 atgcgctcgg cctcggcggt ggcctcggtg gtcgctcgct cgagtgcctg ctcggcgtcg
     8161 ctggtgagct cggtcgcctt cgacctcgcc tcctcggtga gccggtcggc ctcggtggtg
     8221 gcctcccgga tcatccgggc cgcggactcc tcggccttgg accggatctc ctcggcggcg
     8281 cgccgggccg tcgacagcac cgaggcggtc tcggagccga tcagcttcac cagctcgtcc
     8341 tcgtcgagga gcgacagctc gggcggcggc ggtgccgggg cggcgaccgg tgcggccgtg
     8401 tccatgtcgg cgatccgggc ctgcagctcg tcgaccagct cgttcgtcgc cgcgatgcgc
     8461 gccgcgacgg tctcgaggaa ctggtcgacc gctcgggcgt cgtagcgccg cgacgaaccg
     8521 gggaaccgga cgttccggag atcggccggg tcgatgcggg ggtcctcggt catcggggag
     8581 caaccctacg cccggccctc cgagcgccgg tggagccccc gaggagcccc acctggcctg
     8641 catcgtggcc tacggcggcg ccagacccag cacctcgagc aagccctcga gcacctccga
     8701 ggtcggcccc acgaccgggt ccagcagccc gccctccggt ggcgcgggcg gcgtcgtcgt
     8761 ggtggtcgtg gacgtcgggc gggtggtggt cgtgtcggtg ctgacacccg gtgtcgtcgc
     8821 tgtcggcgac cctgcccgcg tggtcgtcgg gctcatccca gcgccgtcgg aggcgtcgac
     8881 ggccagctcg atcgtgtcga gcaccggggc cgcgctcacg ccctggtcga gggcgacgag
     8941 gccccaggca gcgccggcgg cccggaaggc gaagacctcc tgccaccgct cacggaaggt
     9001 cccctgccgg ccctgcacgg tgatggcggc cccgaccagc gtctcacccg gtgtgcgggc
     9061 cgggtcgagc aggtcctcgc ccagctcgcg ctcgtcggcc agggcgggca gcaccgactg
     9121 gaagaacgag agggaccgtc cgctccctgg cgggaggtcg ctggtgtagc cccgggccag
     9181 cgcctcgagc cgctcaccga acgcgatcgc ctcgccgagg taccgccggt cccactcgtc
     9241 gctgccgtcg aagctgatcg gctcgggggt gcccccggcg acgagcacca gccggcgcag
     9301 cccggccagc tcccccacgt caccgacacc ggtgacccgt gcccggccgg cgtaggcgct
     9361 cgcgaccacg tcgtccgcgt ccacccgcag cgctccctgc gcggtcaacg tggcggtgtc
     9421 caccgacacc tgggccggga accccccgac agcgagcagc tcaccagcga ggacccgtgg
     9481 tggtgcaccg acctcgagga ccgtggcggt gtcgtccctc gaccgcagct cgtaggtgga
     9541 gccgtcggcg agctccagct cggcggatcc ggacaccatc gccacctgct ccccgaaggc
     9601 cagcacctcg acctcgtcga cgagctcccg ggtggcgccg tcgaccgccg tcacggacgc
     9661 ggcaccgtcg acctgcaggc gagcctggtc ggggccgggg tcgtcggggt cgtcccggag
     9721 caccacggcg cccacgccca ccagcaggac cacgaccgcg cagccggcga gcacggcccg
     9781 cgtgctcacg gccggtccgc gggtcgatcg agcggcagct ccatgcggac ggtggtcccc
     9841 ttgcccacct ccgagctgca cgacaggtca ccgccgtggg ctcgcacgat gcggctcacc
     9901 agggccaagc cgaggccgag gccgccgaag cggcgggtca tggacccgtc ggcctgggcg
     9961 aagtcctcga agatggactc gcggcggtcc gggtcgatgc cgacgccctg gtcgtcgacg
    10021 gccagcacca ggcgaggtcc ggtgccgttc tcgtgcaccg aggcgctgac ccggatgcga
    10081 ccgccctcgg gcgagtactt cacggcgttg tcgatcagct cgtcgagggc cacgtcgacg
    10141 tagcggcggt cgaggacggc cggcggcatg ccccgaccga tgcgccgggt gaggcggtgc
    10201 gtgtcgggca gccggtcgct ccaccggtcg gccagggcgt cgagcagctg acgggcgtcg
    10261 accggctcct cgtggagctg cagccggccg gcggcgaggg tggcgaactg cacgagctgg
    10321 tcggtgaccc ggagcagctt gcgggcggac tcgtggatct cgccggcgaa gcgcttggtc
    10381 tggtcggggc cgagctcgcg ggtggcgagg atgtcggcgt agccgaggac cggggtcagc
    10441 ggggtgcgca gctcgtggct gacgttcgcg atgaagtcgg tcttcatctg ctcgatctcg
    10501 cgctcgcggc gcacgtcgcg gagcacgaag acggcgccgg tcagcatgcc ggccggaccc
    10561 cgcagcggac cggcggagac cgccaccggc acggtggcac cgtcggatcg gaccaggtcc
    10621 agggcagccg tccacgagtc gaccaccggc ttcacgaagc gcggcgtcac gtcgacgccg
    10681 tcctcgccgg tgatcgtgca caccttcgcc agggtgcggc cgagcacgtc acgggccggc
    10741 aggccgacga gctcctcggc cgaggcgttg aagtcggtga tgcggccccg gtcgtcgacg
    10801 gcgacgagcg cctcgcccat gccgccgacc acaccttcga ggcggccgcg cagggcggac
    10861 tcctggtcgg ccgcggagcg caggtcgtcg gtcatcgact gcagggacgc ggccatctgg
    10921 ttgaaggtcg aggccagcac gccgagctcg tcctcggccc cgacgtcggc ccgggccgac
    10981 agccgcccct gctgcagctc cccggtgacc gtggtcagcc ggctcaaccc ggcgccgatc
    11041 cggttgccgg cgtacccggc caggaccatg gccagcgcgc cggcgccgag ggccaccagg
    11101 aacagcaggc ggaacaggtc ctgacgggtg gcgtcgatgc tgccctgcgg gacgttgacg
    11161 acgacggcca gcttcggggt gccgtcgacc tccacggtgt cggcgacgac gaagcggtcg
    11221 ccgtccaccg tcgtgcgccg ctccccctcg agcaggacgt cgcccgccag ctgccggatc
    11281 acgggttccc cggcctgggg gccgctgctg gcgtagatgc gggaccggtc ggcgatggcc
    11341 atgcccacgt cttggtccac cgaccctccg tcgtcgaccc gatcggccag gtactcgtcg
    11401 tcgagccgtg tcgtgaccac ggcgacgccc acgaactcga tgccgtcggc gtcgaggatc
    11461 ggcgccgcgc cgacggcgaa cgtctggttc ccgacgatgt ccacggcttc gctggactgg
    11521 cgggtctcgc gcacctcagc gacggtggcg tcgccgagca aggacacgcg caccgcatcg
    11581 gacagatcga gcgcggcagc gaggcggccg tcggctccga gcaccgcgat cgggccgggg
    11641 aaccgcagct gcaggagact ggtgcgggcc tccgccagcg ccgccgcgat ctgggctcgc
    11701 gccgcttcgg catcggcggg agcggcggca ggatcggtgg cagtctggat cgctgctgcc
    11761 tggccggcga agacacggcc gagcagctcg gcggatgctc gggccgaccg accagcgtcg
    11821 acgaacacct gcgactcggt ctcagcccgg gcctcgtagc gctcggctgc ctcgtcctcg
    11881 acgttctcgg cgatcacgac cgacagggcg atcgccagga cggtgaccac gaccaggacc
    11941 accgctgcgg cagccatggc gacccgggcg gcgatggcgc gacgggacgc gaccacgagg
    12001 ccgatgccga acagcaccgc gcccagaccc cggacgacgt ctgctccggt ctccagctcg
    12061 gcggcgacga gcccctcggc cgccgccagg gcgagcacgc cggcggcggc cgcgccctgg
    12121 gctcctcggg acgtccgcca ggtcgcgacg gccgcggcga ccgcggcgat gccgccgagg
    12181 cggagcccga cgagcaccgg gtcggtgccg tcgtcgacga ggagggaccc gtgcaggaac
    12241 gccgcggcgg ccagggcgat ggcgccggtg acgagcagga accgcccgag cgcccggcgc
    12301 gccagcagat cggggcgcag ggccgtgacc gcgatcgccg cgatggcggc catgaacagc
    12361 gtgaactcgg cagcgaactg gagctgggcc gcttccgtcg tcatgccagg cctaccggat
    12421 ccgcgaggag ggggtcagcc cacgtcggcg gggttcccca gtccactccc accgagggat
    12481 gccagcgcgt cgagggcatc cgccagggta tcgaccgcga tcacctcgag ggcgtctcct
    12541 gcccgctcct cggcctcctg gaactccttg ctggggacga ggaagacgtc gtagccctgg
    12601 tcgatggcgg cggcggtctt ctgacggacg ccgcccacgt cgccgacgtt gccggccccg
    12661 tcgatggtgc cggtgacggc gacgtcgagg ccgcccgtga gctcgccggg cgtgaggacg
    12721 tcgagcacgg cgagggtgaa ggccagcccg gccgaggggc cgccgacgtt gccgctgtcg
    12781 atgtccacgt cgatcgggaa gtcgtaggcg gggctgcggg gttggagctc gatgccgatc
    12841 aggccctgct ccgggtcggc gggcgaggca ccgagcacca cgggcacgtc ggtcgcgtcg
    12901 ccacgctcgg ggggctcgac cgagagcacg accgtgtccc cgggcgcctt cgggtccatg
    12961 atgcggctca cgtcgtcgat gtcgtcgagg ggctcgccgt cgagggcgac cacggtctgg
    13021 ccgacctcga ccaggccctc accggccgag ccctcctgca ccgtcacgat cgccaccccc
    13081 tcgatggtca ccggcacctc gtagccgagc tcgagcaggg cgacccggat ggcgtcctgc
    13141 ttggacgtgt ccatcagctg gaggttgaac tggcggttct cctgggggga gcggttgccg
    13201 aggatcagct cgtcagccac cacctgcacg tcgtcgtcga accacgccca cacgtaggac
    13261 gccacggtcg actggcgcag cgacaccgtg gtgaacgcga tctcgccggc gggcgggtgg
    13321 acctcgatgc cctcgccgac atcgaccagc gcggtggtgg gccgggccga accggggcgg
    13381 aactcgaagt ggccgatctg caccgtgaac gccgcgaaca gggcggcggc ggtgccgagc
    13441 agggcgaccg cggcgagcca ccacggccag gtgcgccgcc gcgacaccgg ggcatcgggc
    13501 ggctcgggga tgtcccggtc gtggtcacgg gtgctgaccg accccggtac gctctcgtcc
    13561 tcggtgctca ctctcatcct gctcgtggtg ctcggcggtg gcgccggcct gatggccgtg
    13621 tggctgatcg acgcccgcgg gctctcccgg ctgctgggtc ggttcgctcc gggcagccta
    13681 cggccgaccc ggccggcgga cacgacgcgg acggagacgc cggagcccgc ggcccccgag
    13741 ccggcgctga cctccgccgc caccgtggcg accgcctacg agccggtgga gtcgtccttc
    13801 gagcccctcc ccgcaccgga gcccgccacc cgtcgggacc agaagaaggt cccggccgcg
    13861 tacaccgcgg tcgagggcac ctaccgggag gtcgcccggg tcccgctgtg gcgcaagctg
    13921 gtgagcctcg tcgtgctcct cgtcatcctt gcggtggtcg gtgccaccat cgccgccctc
    13981 gccggcgcca cgttcggcct ggtcgccgag ctggtcgacg gcgccgtcgg gtgagcgacg
    14041 acgccaccgc acgccgccgc gacgccgccg tcgccgggca catcggtgac gaggccaccg
    14101 cccgctcgct gctcgccgac gaggaccaca aggtgcgggc caccgccctc gccgcgctcg
    14161 accggatcgg cgcgctgggc gacgacgagc tggcggccgc gctcgccgac cccgcgcccg
    14221 gggtccgccg gcgggcgctg tcgttgaccg ccacccgacc gggcgacgag ccgcccgcag
    14281 cgctggcgct gctcgacgac gaggatcccg tcgtggccga gaccgccgcc tgggccgccg
    14341 gcgagcgcca gccccccgaa gacggcgcag ccgaggcact ggcccggttg gccacggagc
    14401 accccgagca cctggtccgc gaggcggcgg tggcggccat cggtgcggtg ggcgacccgg
    14461 tgggcctgcc ggccgtgctg ctcgccctgg agggacgggc caccctgcgc cgacgggc
//
LOCUS       DATISU010000347         1937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_537834, whole genome shotgun sequence.
ACCESSION   DATISU010000347 DATISU010000000
VERSION     DATISU010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537834"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1128
                     /gene="pheA"
                     /locus_tag="VLR27_13805"
     CDS             154..1128
                     /gene="pheA"
                     /locus_tag="VLR27_13805"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HSP04580.1"
                     /translation="MTGSDTSATRPAADGRGRIAFLGPEGTFSEQAMLTQPDYAAMDL
                     VPCGTITDVLHAVTSGEVEIGFVPIENAIEGSVNITLDALIFDHELRMQREVVIPIVM
                     CLLAPPGVGIADIERIVSIPVATAQCRGFLREHLPSVEHVPANSTALAAQIVAEEDDG
                     RSAAIGTSLAGKVNGLEVLAEDIEDHRGNETRFVAVTRGGVPAPTGHDKTTIVVFQRA
                     DRPGSLLSILQEFAARAINLTRLESRPTKRGLGDYCFIIDLEGHVGDELVADALRDLK
                     SKNEDVLFLGSYTAAGETAPRVRREAEAAWREANDWVQALRTEIPPAT"
     gene            1216..1356
                     /locus_tag="VLR27_13810"
     CDS             1216..1356
                     /locus_tag="VLR27_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04581.1"
                     /translation="MVRPTSGTTTSTSRTATSGTGSEFRSLAAATSMERRPHANARAA
                     LA"
     gene            1499..1768
                     /locus_tag="VLR27_13815"
     CDS             1499..1768
                     /locus_tag="VLR27_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04582.1"
                     /translation="MDLDALAHARIGGEQDPSTDDTVDPAMVDALRVIRSLEVPTGAL
                     TRDRTQQIHAGRAFVLAVGIVLVLVTVFTLNQLVQAAFQDLGQGL"
     gene            1843..>1937
                     /locus_tag="VLR27_13820"
     CDS             1843..>1937
                     /locus_tag="VLR27_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HSP04583.1"
                     /translation="MKDSVGQYLHEIGQVPLLDAARERELAQRIEA"
BASE COUNT          289 a          730 c          652 g          266 t
ORIGIN      
        1 gcccggtggt ggtcgacccg ggcgtggccg aggtcggcga aggggaggcg gcgcaggcgg
       61 gtgaccgcgg cgtccggatc gacgcggccg gcggcgacgt cgtcgagaag ctcccgcagg
      121 gtgtgggcgt ccaccgtcct atcctggccc gtcgtgaccg gatccgacac gtctgcgacc
      181 cgtcccgccg ccgatggccg gggtcgcatc gcgttcctcg gccccgaggg caccttcagc
      241 gagcaggcga tgctcaccca gcccgactac gccgcgatgg acctggtgcc ctgcgggacc
      301 atcaccgacg tcctccacgc cgtcacctcc ggcgaggtgg agatcggctt cgtgccgatc
      361 gagaacgcca tcgagggctc ggtcaacatc accctcgacg ccctcatctt cgaccacgag
      421 ctgcgcatgc agcgcgaggt cgtcatcccc atcgtcatgt gcctcctcgc cccacccggg
      481 gtaggcatcg ccgacatcga gcgcatcgtg tcgatcccgg tcgccaccgc ccagtgccgc
      541 ggcttcctgc gcgagcacct gcccagcgtc gagcacgtgc cggccaactc gacggccctc
      601 gcggcgcaga tcgtggccga ggaggacgac gggcgctcgg cggccatcgg cacgtcgctc
      661 gccggcaagg tcaacgggct cgaggtgctg gccgaggaca tcgaggacca ccgcggcaac
      721 gagacccgct tcgtcgccgt cacccggggc ggcgtcccgg cgcccaccgg gcacgacaag
      781 accaccatcg tggtgttcca gcgcgccgac cgccccggtt cgctcctgtc gatcctccag
      841 gagttcgccg cccgggccat caacctgacc cgcctcgaga gccgccccac caagcggggc
      901 ctcggcgact actgcttcat catcgacctc gagggccacg tcggcgacga gctggtggcc
      961 gacgcgctcc gcgacctcaa gtccaagaac gaggacgtgc tcttcctggg cagctacacc
     1021 gcagccggcg agaccgcccc ccgggtccgc cgggaggccg aagcggcctg gcgggaggcc
     1081 aacgactggg tccaggccct gcgcaccgag atcccacccg ccacctgacg caccccctcc
     1141 agaactggca gttctggtgt tgcggtggtg cggggtacgg gacggcctcc cgccccgacc
     1201 ccggaggtcg ctcccatggt ccgccccacg tctggcacga cgacatcgac cagcaggacc
     1261 gccacgtcgg gcaccggctc ggagttccgc tcgctcgccg cagccacgtc catggagcgc
     1321 cggccccacg ccaacgcccg cgccgccctc gcgtgaccgg cggtggtgct cgtgcaccac
     1381 gcgggtggtg cgcccggccc agcgcgtggg tgacctgagc ctctgacgcc gacggcagac
     1441 gtctgcgcac gatcgctcga aggacgcgca cggagcgcgc cgctgggagg gcacggacat
     1501 ggatctcgac gcgctcgcgc atgcgaggat cggcggcgaa caggacccgt cgacggacga
     1561 caccgtcgac ccggcgatgg tcgatgccct gcgcgtcatc cggtcgttgg aggtgcccac
     1621 gggcgccctg acccgagacc gcacgcagca gatccacgcc ggcagggcct tcgtcctggc
     1681 cgtcggcatc gtcttggtcc tggtgactgt tttcacgctg aaccagctcg tgcaggcggc
     1741 cttccaggat ctcggccagg gtctctgacc cgccgagctg aaaccgaccc gcaccggaat
     1801 gacggcggca ctgccgctgt tcttctccct gaggtgaaga acatgaagga ttccgtaggt
     1861 cagtacttgc acgagatcgg ccaggtgccg ctgctcgatg ccgcccggga gcgtgagctc
     1921 gcgcagcgga tcgaggc
//
LOCUS       DATISU010000348         8477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_72360, whole genome shotgun sequence.
ACCESSION   DATISU010000348 DATISU010000000
VERSION     DATISU010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8477
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72360"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VLR27_13825"
     CDS             <1..862
                     /locus_tag="VLR27_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP04584.1"
                     /translation="EVRIGDTLVSGPGTQVPPERRRIGMVFQDWALFPHLTVAGNVSF
                     GLPRAERKGPRVEAALELVGLEGFGDRMPGTLSGGQQQRVALARALAPEPSALLLDEP
                     FSNLDTTLRARVRTEVHELLHRVGITTVFVTHDQDEAFVLGDDVAVMSEGLIVQQAPP
                     HELYADPANAWVAHFVGDANLVPGVARQGRASTALGDVDLRSEVEGSCEVLLRPEDLA
                     LDSAAGDGASKPVHVELVEYYGHDTVYLVTLDDGTRVRVREVSVPRFDRGDVASIRTT
                     GRAAVAFAAG"
     gene            877..2214
                     /locus_tag="VLR27_13830"
     CDS             877..2214
                     /locus_tag="VLR27_13830"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSP04585.1"
                     /translation="MHVVIGAGPAGLATAWQLQRDGHEVRVLEASDHVGGMAGVREVA
                     GVRVDLGSHRLHPSTPPWLLDELGQLVELQERPRNGRIRMGGRWVPFPLTPVGLVRGL
                     PARVAARAAIDAVAAPLRRPRADTYAEVVRAGLGPTVWRRFHEPYAWKLWDTDPDLLA
                     GELARRRVSASSPVDIARRLLRGVRSTPTFLYPRRGFGAIAEALAEQVPVDTGRRVNR
                     IIEHHDRVIVGLSDGRLVTASHVHATVPAWRVASWLGLHEDPLGPPPVRAMVLVYLVL
                     DRRPYTTFDAHYLPDRNVLLSRLSEPTNYRDNPEDPTDRTVLCAEIPCWSGDEVWTAS
                     ADVLGARVADDLVRSGLPDPRHVEVHVERLPSVYPVMTPQSLGALARAERALTMSARV
                     TVLGRQGLATPDNTHHVLEMGVRSARAVRPDGTFDHAAWADAREGFRTYVVED"
     gene            2265..3086
                     /locus_tag="VLR27_13835"
     CDS             2265..3086
                     /locus_tag="VLR27_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04586.1"
                     /translation="MLVDRIPARTAGRIMVVTAAVGLVVAVIGTVVAWQLVGQVGGAV
                     DESLAITDESVDTIEDTIVVAGQVVDDVGAALGALDDVLVQLRDGTRDVEPVLGDVED
                     LTTDVPTALGAFQDTLDRVAGAAGDVDAILVQLARLPLAPSYDPETTLSAQLDQLSDD
                     LDPVIETLAASGDDLAALRATTSELRAELGGLTSDVRALVTRMDESSALIDRYQAQAG
                     RAGDLVDGSRDDLDGSVTAMRVLVILGGLVFAAGQLVPLWVGSGLLSSDPTGRDG"
     gene            complement(3060..3830)
                     /locus_tag="VLR27_13840"
     CDS             complement(3060..3830)
                     /locus_tag="VLR27_13840"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSP04587.1"
                     /translation="MPAAGAPVVLFLPARDEVATVADVLARAPRAVTVDGRSHPVRTL
                     VVDDGSVDGTGDAARACGAEVVRTPGLGLGAAVGIGLAWGASVGAAAVAFCDADLEYD
                     PAELEDLVAPVLTGRADYVVGSRFLGGARRMKPHRELGNRFLTALTAASTAGRRAVPL
                     TDGQSGYRALSGEAAATTVVAHDYNYAQVLTLDLLGRGYRYLEVPITYAHRQVGRSFV
                     RLPTYLRRVLPTMWRVRRSWAVTAERPGPASTVAPRRI"
     gene            3876..4904
                     /locus_tag="VLR27_13845"
     CDS             3876..4904
                     /locus_tag="VLR27_13845"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSP04588.1"
                     /translation="MPAPRTRLPRAWKGVLASLCAVALVAPACSTDDDRLTIYSGRTS
                     DLIKPLLDQFSEDTGTKIDVRYGDSADLALLIDEEGDRSEVDVFISQSPGAVGFLDEE
                     GVLSELDQSVLDLVDDDFHAGDGEWVGLSGRVRVLVYNTDLVEESELPGSVLDLTEDE
                     FAGRVAVAPNNGSFQDFVTAMRIELGDDAATEWLEGMVANDSPTYSNNTAIVEAVTRG
                     EVPMGLVNHYYLERALNDDPSLPAANHFFDEGDMGSLLITTATAIIEGSDQSEDAQSL
                     VEFLLSEEAQRYFSEETFEYPLARDVPQAEAVPALSTINTTTIDLDGLGGGLARTAEL
                     IQDSGLDR"
     gene            4916..6490
                     /locus_tag="VLR27_13850"
     CDS             4916..6490
                     /locus_tag="VLR27_13850"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HSP04589.1"
                     /translation="MRRRAPVALSATSVLVALVFATPLAYLISRVAVGDVRMSSVVSD
                     DRVQDALWRTIVLASLVAVSAAVIGTALAWLVARSDVPYRRVFAVLVPLPLVFPSFVG
                     ALALRASVSPGGLLESVLGPFGVDRLPQVDGLFGSWLVLTLFTYPYVQLPVAARLRAL
                     GPSVEESARLLGRRPLAVFFQVVLPQARTAIAAGTLLVFLYAVSDFGAVDLLGYPALT
                     RVIYANRVLRRDLAMALGLVLAIVALLVTIAERRVGRNAAVAQGLRTKRSLTVSLGRW
                     RWPVFGLVVFFLLNAIVAPVASLVHWTRRGITSGSLEVGDADLWTPVLNTAGISMVTA
                     LVAVVVVLPLAFLATRYRSRVAGPALTMVVAGFALPGIVIALSMVFLSLNTPVLDAFY
                     QTFPLLVLAYVVHFGAQSVRAASVAVGSVPARLDDAARMLGAERWRRLLRIDAPLMAP
                     GLAAGGGLVLLSTMKELPATLLLAPTGFETLATRIWSATEENALAEAGLAALVLLVVS
                     GLLTWVLVVRRAELLD"
     gene            6495..7970
                     /locus_tag="VLR27_13855"
     CDS             6495..7970
                     /locus_tag="VLR27_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04590.1"
                     /translation="MSLRRLLAAVFAVVALWALLGAGVRATYGARVTADEPQYLLSAI
                     SIVEDGDLDIADELAAERWRDFHDAQLPRQTEPRSDGSELSPHDPLLPVLLAPGVAVA
                     QLVAGDWQHAELAGARVVLALVAGALAAIVVWTAVAHLGVRRSTAVVTVGAFALAAPL
                     STYGNQLYPELVAALAVAVGAAALLELAAAAEHRRWGPTAAWVGAVVALPWLGVKYAA
                     VAAAIAVLGLLFDRRRRIVALVGLVAAGIAYLVVHQRVYGGWTVYAAGDHFVDGELTV
                     VGNDPDHLGRTVRLAGLLVDRHFGLLVWAPAYLLAVPAVGALLRRRDRIAAVLLLPLA
                     AGWATATWVALTMHGWWWPGRQVVVVLPLAVLAVAAFVDGERRAGSRWMWLALLVTGA
                     LGIATWVWLAVEATFGHLRLIVDFDRTTNPWVRAARLVLPDLREGGAMTDLVLSTWTV
                     VLLALAVWGWRRGGSGTRGAPQASASQPGSGSAPEPSSAVE"
     gene            complement(7956..>8477)
                     /locus_tag="VLR27_13860"
     CDS             complement(7956..>8477)
                     /locus_tag="VLR27_13860"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HSP04591.1"
                     /translation="TDHPLIGFVVAFTVWDGLGWVDHWIGHRTRIGWVTHRPHHGGSS
                     FNLTLALRQSPYPLAGIALLPMVALTGVPFETAAAVVAVSNTWQAVIHTQARLPLPGW
                     LERTVMTPATHEIHHDHGGLVNLGPVLTLWDRAAGTFCTPTTGASAVGPARGGRDVHQ
                     PGTTPDLDRSLDR"
BASE COUNT          977 a         3201 c         3056 g         1243 t
ORIGIN      
        1 cgaggtccgc atcggcgaca ccctcgtcag cggccccggc acccaggtcc cgccggagcg
       61 gcgccgcatc ggcatggtgt tccaggactg ggcgctgttc ccgcacctca ccgtcgccgg
      121 caacgtgtcc ttcggcctgc cccgcgccga gcgcaagggg ccacgggtgg aggctgcgct
      181 cgagctcgtc ggcctcgagg gcttcggcga ccgcatgccg ggcaccctgt cgggcggcca
      241 gcagcagcgg gtggcgctcg ctcgggcgct cgcccccgaa ccctccgcgc tgctgctcga
      301 cgagccgttc tccaacctcg acaccaccct gcgggcgcgg gtccgcaccg aggtccacga
      361 gctgctgcac cgggtcggca tcaccacggt gttcgtcacc cacgaccagg acgaggcctt
      421 cgtgctcggc gacgacgtgg ccgtgatgtc cgagggcctc atcgtccagc aggccccgcc
      481 gcacgagctc tacgccgacc cggccaacgc ctgggtcgcc cacttcgtcg gcgacgccaa
      541 cctcgtgccc ggcgtcgccc gccagggccg ggcctccacc gccctcggcg acgtcgacct
      601 gcgcagcgag gtggaggggt cctgcgaggt gctgctgcgg cccgaggacc tcgcgctcga
      661 ctcggccgca ggtgacggcg ccagcaagcc ggtgcacgtc gagctggtgg agtactacgg
      721 ccacgacacc gtgtacctcg tgaccctgga cgacggcacc cgggtgcgcg tgcgcgaggt
      781 ctccgtgccc cgcttcgatc gcggcgacgt ggcctcgatc cgcaccaccg gccgcgccgc
      841 ggtcgcgttc gccgccggct gagcgccggg agcgccgtgc acgtcgtcat cggagcagga
      901 ccagcgggcc tggccacggc gtggcagctg cagcgagacg gccacgaggt ccgcgtcctc
      961 gaggcatcgg accacgtcgg cggcatggcc ggcgtccgcg aggtcgccgg cgtccgggtc
     1021 gacctcggca gccaccgcct gcacccgtcc acgccgccct ggctcctcga cgagctcggc
     1081 cagctggtgg agctgcagga gcgcccccgc aacggccgca tccggatggg cggccggtgg
     1141 gtgccgttcc cgctgacgcc cgtcggtctc gtgcgcgggc tgcccgcccg ggtcgccgcc
     1201 cgcgccgcca tcgacgccgt ggccgccccg ctgcggcgac cgcgggccga cacctacgcc
     1261 gaggtcgtcc gcgccggcct cgggccgacg gtgtggcgcc gcttccacga gccctacgcc
     1321 tggaagctgt gggacaccga cccggacctg ctcgccggcg agctggcccg ccggcgggtg
     1381 tcggcgtcgt cgcctgtcga catcgcccgg cgcctcctcc gtggggtgcg ctcgacgccg
     1441 acgttcctgt acccccgccg cgggttcggc gccatcgccg aggccctggc cgagcaggtg
     1501 cccgtggaca cgggccggcg ggtgaaccgc atcatcgagc accacgaccg ggtcatcgtc
     1561 gggctctccg acggccggtt ggtgacggcc agccacgtcc acgccaccgt gcccgcctgg
     1621 cgggtggcgt cgtggctggg gctgcacgag gaccccctcg gcccgccgcc ggtgcgggcc
     1681 atggtgctcg tctacctggt gctcgaccgc cggccctaca cgaccttcga cgcccactac
     1741 ctgcccgacc gcaacgtgct gctcagccgc ctgtcggagc cgacgaacta ccgcgacaac
     1801 cccgaggacc ccaccgaccg cacggtgctc tgcgccgaga tcccgtgctg gtcgggcgac
     1861 gaggtgtgga cggcgagcgc cgacgtgctg ggcgcgcggg tggccgacga cctcgtccgc
     1921 agcgggctgc cggatccccg ccacgtcgag gtccacgtgg agcgcctgcc gtccgtgtac
     1981 ccggtgatga ccccgcagtc gctcggcgcc ctggcccggg ccgagcgcgc cctcaccatg
     2041 tcggcacggg tcacggtgct cggccgccag ggcctggcca cgcccgacaa cacccaccac
     2101 gtgctggaga tgggtgtgcg gtccgcccgg gccgtccggc ccgacggcac cttcgaccac
     2161 gcggcctggg ccgacgcccg cgagggcttc cggacctacg tcgtggagga ctgatgagct
     2221 gctcggttgg gctgcgccgg cgggcgatcg ggtagcaggg ggaggtgctc gtcgatcgca
     2281 tcccggcccg gaccgccggc cggatcatgg tcgtgaccgc cgcggtcggg ctggtcgtcg
     2341 cggtgatcgg caccgtcgtc gcctggcagc tggtcggcca ggtcgggggc gccgtggacg
     2401 agtcgctggc gatcacggac gagtcggtcg acacgatcga ggacaccatc gtcgtggccg
     2461 gccaggtggt cgacgacgtc ggcgccgcgc tcggcgcgct cgacgacgtc ctcgtccagc
     2521 tccgggacgg cacccgcgac gtcgaacccg tcctcggtga cgtggaggac ctcaccaccg
     2581 acgtccccac cgccctcggc gccttccagg acaccctcga ccgggtcgcc ggtgcggctg
     2641 gcgacgtcga cgcgatcctc gtgcagctcg ctcgcctccc cctcgccccg agctacgacc
     2701 cggagacgac gctctcggcc cagctcgacc agctgtccga cgacctcgac ccggtcatcg
     2761 agacgctggc ggcatccggc gacgacctcg ccgcgctgcg ggccaccaca tcggagctgc
     2821 gcgccgagct cgggggcctc acctccgacg tccgcgccct cgtcacccgc atggacgaga
     2881 gctccgcgct gatcgatcgc taccaggccc aggccgggcg agcgggcgac ctcgtggacg
     2941 gcagccgcga cgacctggac gggagcgtca cggccatgcg ggtgctggtg atcctcggtg
     3001 gcctggtgtt cgccgccggc cagctcgtcc cgctgtgggt cggttcgggg ctgctgtcgt
     3061 cagatccgac ggggcgcgac ggttgatgcc gggccgggac gctcggccgt caccgcccac
     3121 gaccgccgga cccgccacat cgtgggcagc acgcgacgca ggtaggtggg gaggcggacg
     3181 aaggaccggc cgacctggcg gtgggcgtag gtgatcggca cctcgaggta gcggtagccg
     3241 cgacccagca ggtcgagcgt gagcacctgc gcgtagttgt agtcgtgggc caccacggtc
     3301 gttgccgccg cctcgccgga gagggcgcgg tacccggact gcccgtcggt cagcgggacc
     3361 gcccgccgac cggccgtgct cgccgcggtg agggcggtga ggaagcggtt gcccagctcg
     3421 cggtggggct tcatccgccg tgcgcccccc aggaaccggg accccacgac gtagtcggcg
     3481 cggccggtga gcaccggtgc gaccaggtcc tccagctcgg cggggtcgta ctcgaggtcg
     3541 gcgtcgcaga acgccaccgc agccgcaccg accgacgcgc cccacgccag accgatgccc
     3601 acggcggcgc cgaggcccaa ccccggggtg cgcacgacct cggccccgca ggcgcgcgcc
     3661 gcgtcgccgg ttccgtcgac cgacccgtcg tcgaccacga gcgtgcgaac cgggtgcgag
     3721 cgaccgtcga cggtgacggc acgaggggcc cgggcgagga cgtcggcgac ggtggcgacc
     3781 tcgtcccgag cgggcaggaa cagcacgacg ggggcaccag cggctggcat taggcgaact
     3841 ttacacacaa gggtcggcct cctagcatcc ggtccatgcc cgccccccga acccgcctgc
     3901 cccgcgcctg gaagggcgtg ctggcgtcgc tgtgcgcggt ggctctcgtc gcgccggcct
     3961 gctccaccga cgatgaccgc ctcaccatct acagcggccg cacgagcgac ctcatcaagc
     4021 ccctgctgga ccagttcagc gaggacaccg gcaccaagat cgacgtgcgc tacggcgact
     4081 cggccgacct cgcgctgctc atcgacgagg aaggcgaccg cagcgaggtc gacgtgttca
     4141 tctcccagtc gcccggggcc gtcgggttcc tggacgagga gggcgtgctc agcgagctcg
     4201 accagtcggt gctcgacctg gtcgacgacg acttccacgc cggcgacggg gagtgggtcg
     4261 gcctctcggg ccgggtgcga gtgctcgtct acaacaccga cctcgtcgag gagtccgagc
     4321 tgcccgggtc ggtgctcgac ctgaccgagg acgagttcgc gggtcgggtg gccgtcgccc
     4381 ccaacaacgg ctcgttccag gacttcgtca ccgccatgcg catcgagctg ggcgacgacg
     4441 cggccaccga gtggctggag ggcatggtcg ccaacgactc gccgacgtac tccaacaaca
     4501 ccgccatcgt cgaggccgtc acccgcggcg aggtccccat ggggctggtg aaccactact
     4561 acctcgagcg cgcgctgaac gatgatccca gcctgccggc ggccaaccac ttcttcgacg
     4621 agggcgacat gggctcgctg ctcatcacga cggccaccgc gatcatcgag ggcagcgacc
     4681 agtccgagga cgcccagtcc ctcgtcgagt tcctcctgtc cgaggaggcc cagcgctact
     4741 tcagcgagga gaccttcgag taccccctcg cccgtgacgt gccccaggcc gaggccgtgc
     4801 ccgccctgtc gacgatcaac accaccacca tcgacctcga cgggctgggc ggcgggctcg
     4861 cccgcaccgc cgagctgatc caggacagcg gcttggaccg ctagtgctcg acacgatgcg
     4921 ccgccgagca ccggtcgccc tgtcggcgac ctcggtgctc gtcgcgctcg tgttcgccac
     4981 gccgctggcg tacctgatct cccgcgtcgc cgtgggcgac gtgcgcatgt cctcggtcgt
     5041 gtccgacgac cgggtgcagg acgccctgtg gcgcaccatc gtcctggcct cgctggtggc
     5101 cgtgtcggca gcggtcatcg gcaccgcgct ggcgtggctg gtggcgcgct ccgacgtccc
     5161 gtaccggcgg gtgttcgccg tgctggtgcc gctgcccctc gtgttcccgt ccttcgtcgg
     5221 cgcgctcgcg ctgcgggcgt cggtgtcgcc cggcgggctg ctggagtcgg tgctcggacc
     5281 gttcggggtc gaccggctcc cgcaggtcga cggtctgttc ggctcgtggc tggtcctgac
     5341 gctgttcacc tacccctacg tgcagctgcc cgtggccgcc cgcctgcgcg ccctgggtcc
     5401 ctcggtcgag gagtcggccc ggctgctcgg tcgacggccg ctggcggtgt tcttccaggt
     5461 ggtgctgccc caggcccgca ccgccatcgc cgccggcacg ctgctcgtgt tcctgtacgc
     5521 ggtgtcggac ttcggtgcgg tcgacctcct cggctacccg gccctcaccc gggtcatcta
     5581 cgccaaccgg gtgctgcgcc gcgacctggc gatggcgctc ggcctcgtgc tggccatcgt
     5641 cgccctgctc gtgaccatcg ccgagcggcg ggtggggcgc aacgccgcgg tggcccaggg
     5701 gctgcgcacg aagcgctcgc tcaccgtctc cctcggtcgc tggcgctggc cggtgttcgg
     5761 cctcgtcgtc ttcttcctcc tcaacgccat cgtcgccccg gtggcgtcgc tcgtgcactg
     5821 gacccggcgg ggcatcacct cgggctcgct cgaggtgggc gatgccgacc tctggacccc
     5881 ggtgctgaac accgccggca tctcgatggt gaccgcgctc gtggcggtgg tggtcgtgct
     5941 gccgctcgcg ttcctcgcca cccggtaccg cagccgggtc gccggccccg cgctcacgat
     6001 ggtggtggcc ggcttcgcgc tgccgggcat cgtcatcgcg ctgtcgatgg tgttcctgtc
     6061 gctgaacacc ccggtgctcg acgccttcta ccagacgttc ccgctgctgg tgctggccta
     6121 cgtcgtgcac ttcggagccc agtcggtccg ggcggccagc gtcgcggtcg gctccgtgcc
     6181 cgctcgcctc gacgacgccg ctcgcatgct cggcgccgag cgctggcggc gcctgctgcg
     6241 catcgacgcg ccgctgatgg caccgggcct ggccgccggc ggcggcctgg tgctgctgtc
     6301 gaccatgaag gagctgccgg ccacgctgct gctggccccg accgggttcg agaccctcgc
     6361 cacccgcatc tggtcggcca ccgaggagaa cgccctggcc gaagccggtc tggccgcgct
     6421 cgtcctgctg gtcgtgtccg gcctgctcac gtgggtgctg gtcgtgcgcc gcgccgagct
     6481 gctcgactga tcccgtgtcg ctgcgccggc tgctcgcggc cgtgttcgcc gtggtcgcgc
     6541 tgtgggcgct gctcggcgcc ggggtgcggg ccacctacgg cgctcgggtc accgcggacg
     6601 agccgcagta cctgctgagc gccatcagca tcgtcgagga cggcgacctc gacatcgcgg
     6661 acgagctcgc cgccgagcgc tggcgggact tccacgacgc ccagctgccc cgccagaccg
     6721 agcctcgttc cgacggctct gagctcagcc cccacgaccc gctcctgccc gtgctcctgg
     6781 cccccggggt cgccgtcgcc cagctggtcg ccggtgactg gcagcacgcc gagctcgccg
     6841 gcgcccgcgt cgtgctggcg ctggtggccg gcgcgctggc cgcgatcgtg gtgtggaccg
     6901 cagtcgctca cctcggggtc cgccggtcca ctgcggtcgt gacggtgggc gccttcgcgc
     6961 tcgccgcacc gctgagcacg tacggcaacc agctctaccc ggagctggtc gccgcgctcg
     7021 cggtcgctgt cggggcggcg gccctcctgg agctggcagc ggctgcggaa catcgacggt
     7081 gggggccgac cgccgcgtgg gtcggtgcgg tcgtggccct gccgtggctc ggcgtgaagt
     7141 acgccgccgt cgcggcggcg atcgccgtgc tcggcctgct cttcgaccgc cggcgccgga
     7201 tcgtcgccct cgtggggctc gtcgcggccg gcatcgccta cctggtggtg caccagcggg
     7261 tgtacggcgg ctggaccgtc tacgcagccg gcgaccactt cgtcgatggc gagctcaccg
     7321 tggtgggcaa cgaccccgac cacctcggcc ggaccgtgcg tctcgccggc ctgctcgtcg
     7381 accgccactt cggcctgctc gtgtgggcgc ctgcgtacct gctggcggtg ccggcggtcg
     7441 gggcgctgct ccggcgccgc gaccggatcg ctgccgtgct gctgctcccg ctggcagctg
     7501 ggtgggcgac ggcgacgtgg gtcgcgctca ccatgcacgg ctggtggtgg cccgggcgcc
     7561 aggtcgtcgt cgtcctgccg ctcgcggtcc tggccgtcgc cgcgttcgtc gacggcgagc
     7621 gccgcgccgg cagccggtgg atgtggcttg cgctcctggt cacgggggcg ctcgggatcg
     7681 cgacgtgggt gtggctcgcc gtcgaggcca ccttcgggca cctgcgcctg atcgtggact
     7741 tcgaccgcac cacgaacccg tgggtgcggg ccgcccgcct cgtcctgccg gacctccggg
     7801 agggtggcgc gatgacggac ctcgtgctct cgacgtggac ggtcgtcctg ctcgccctgg
     7861 ccgtgtgggg gtggcgtcgg ggcggttccg ggacgcgagg cgcgccgcag gcgagcgcgt
     7921 cgcagcccgg ttctggctcg gcccccgaac cgtcatcagc ggtcgagtga tctgtcgagg
     7981 tcgggcgtcg tccccggttg gtgcacgtcc cgcccccctc gcgccgggcc caccgccgaa
     8041 gcgcccgtcg tcggagtgca gaaggtccca gcggcccggt cccagagggt gaggacaggg
     8101 ccgaggttca ccaacccgcc gtggtcgtgg tggatctcgt gggtggcggg cgtcatgacc
     8161 gtccgttcca gccaaccggg taggggcagc cgggcttgcg tgtggatcac cgcctgccag
     8221 gtgttcgaga cggcgacgac ggccgcggcc gtctcgaagg ggactccggt cagcgcgacc
     8281 atcggcagga gggcgatccc ggcgagcggg tagggcgact gtcgcagcgc gagggtcagg
     8341 ttgaaggagc tgccgccgtg gtggggccgg tgggtcaccc agccgatccg cgtgcggtgc
     8401 ccgatccagt ggtcgaccca gccgagcccg tcccacaccg tgaacgccac cacgaacccg
     8461 atgagcgggt ggtcggt
//
LOCUS       DATISU010000349        20996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_351557, whole genome shotgun sequence.
ACCESSION   DATISU010000349 DATISU010000000
VERSION     DATISU010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 20996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20996
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351557"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1932)
                     /locus_tag="VLR27_13865"
     CDS             complement(<1..1932)
                     /locus_tag="VLR27_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04592.1"
                     /translation="MRRRHIRATVALGLAGLAATTFAVLPSAHAEDQRTPADLVADEV
                     ATAAGACDDLDTRLCLLPFPNDQFTTLDATTPTGRRVDLSPLATPRAFGAKPMDPTEW
                     NRNDGFSPGSMILTFVPGLDLTTTFGLDPEPESGAGSTTAGDPFAPRDALPDAVVEAP
                     AMSMAPDAPIVLLDADTGERHPYWAELDEHEVTLAEGHDRTLIIRPLTNLEEGRRYIV
                     ALRDLRAADGTELAPTGAFTALRDAYEPPRNPKGKPKKPKFPPAADIDPADRAGRYQD
                     IFTRLDASGVAVDDLHLAWDFHVASAPNLAGRALAIRDDAFAQLGDTDLADLVPAGAA
                     PAMTISEHVDEVDAAGNVTRTVHGSVTVPNYLTTPQDELIELAGELPVVLPQSRFVYA
                     SPTPGPMDTPIQNPLVPTMEAEFTCKLPVGVAGGAKPTLYGHGLLGGRGETEGGSTAD
                     LRRVGHAMCGVDWIGMATEDIVNVAAILHDISFFPSLADRAQQGFLGFLYVGRALIHP
                     DGVSTHPAFQDAEGRSLLDTSELFYDGNSQGGIMGGALTALAPDFTKAVLGVPGMNYS
                     TLLNRSVDWEGDFIDPTEPDIPAYASFNYNSYPDKVGQQLVFALLQMLWDRAEANGYA
                     HHMTSDPYPNTPPHQVLLEV"
     gene            complement(2045..2518)
                     /locus_tag="VLR27_13870"
     CDS             complement(2045..2518)
                     /locus_tag="VLR27_13870"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSP04593.1"
                     /translation="MPELDEQVVAALDAAVLGWLATADEHGMPNVSPKEVFAHLGGDR
                     VVIAHIASPRSARNAKANPQGCFSVVDVFRQRGFKLTGALHLHAPDDERFDAVAGPLR
                     VMVGDRFALRGVFELTVEAVAPIVAPSYTFHADEVSEESMVAGAMKAYGVRPASP"
     gene            complement(2511..3134)
                     /locus_tag="VLR27_13875"
     CDS             complement(2511..3134)
                     /locus_tag="VLR27_13875"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HSP04594.1"
                     /translation="MLIRDWDRPIDEAEWRAFVTANAFGHLVAGGRDRTVPIIVPTQF
                     VLDTDEVVLHLARPNPIWEAIAENPTVVLSVAGDWAYVPSEWKAIGDEDPRRGIPTTY
                     YAAVQITGDAVVVDDPDEIAGVLRRQLGDLQPEVDAVDPLEHGAQLRAIRGLRIPIDD
                     VRAKFKYGANVDDAHRRAVHERLLARGGPGDASAARHLERRLGADRA"
     gene            3201..3809
                     /locus_tag="VLR27_13880"
     CDS             3201..3809
                     /locus_tag="VLR27_13880"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP04595.1"
                     /translation="MGDDRWNHNIHLHRVVLDAVPPGAQRALDVGCGEGMLSRDLHET
                     IPEVVGLDLHGPSLDLARSQSEGDGITYVLGDVLDHPFELASFDVVASIAALHHMDTV
                     AGLEAMRDLLRPGGVLVVVGLARTRFPRDLGWQVAGAVSTRWLKLSPKRTYWEHSAPI
                     VWPPPETYAGMRTIAERVLPGVAYRRHVLWRYSLVWTKPATR"
     gene            complement(3816..4721)
                     /locus_tag="VLR27_13885"
     CDS             complement(3816..4721)
                     /locus_tag="VLR27_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04596.1"
                     /translation="MQQRPFDAPVTIEGPERAPRQMLAEQTYGGHASLHDESTADSLG
                     LSGAPIEGPTHFSQFDPLAHLLWGDAWFEHGCISAHFRTMVVEGEGVTARATRTSSTR
                     ADISAAKADGSPVLAGSITAAPDVPTALDERRAKLGDPGELFIVDQLAVGDRSGPVTS
                     SVDLKTPNGDLYPFSLEQKLDAITEPSDWYRTERNPWGRPILPMEMVSVLTQKVGADF
                     PVRGPSVGLFLDLEVRLVAGPIFVGHEYVLEREVVALGQSRRTESSWVRTDVTDAESG
                     DLVAHVLLHSGVFKESYAGYPTDRL"
     gene            complement(4770..4988)
                     /locus_tag="VLR27_13890"
     CDS             complement(4770..4988)
                     /locus_tag="VLR27_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04597.1"
                     /translation="MNNAGARAAGLLAVAVLPVAAGISGRDYGDPAALTDGFRVAVVI
                     TAVLAASGGALAAFTIDDDVLAAGAEVV"
     gene            5119..6201
                     /locus_tag="VLR27_13895"
     CDS             5119..6201
                     /locus_tag="VLR27_13895"
                     /inference="COORDINATES: protein motif:HMM:NF022470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HSP04598.1"
                     /translation="MDLEVKRDDLHETRVVDSPPAPLEEGEARLAVRRFGFSANNVTY
                     AVFGDLMGYWTFFPAAPDGEGWGRIPVWGFAEVVEANGSGLDDGELVYGYLPMGTELV
                     VTPTRVGDHGFTDASAHRAGLPPAYNGYTRCATDPVYDPATEPLQMLLRPLFMTDFVL
                     DDWLADNAFFGAGDGTVVLSSASSKTSFGLAHLLARRADRPQVVGLTSAGNVAFVEGL
                     GVYDRVITYDDVATLPADGPSVYVDMAGDAGVRKAVHEQMGGLAASVQVGGTHWEQVG
                     GGGDLPGPAPALFFAPDQIVKRREDWGPGGVESRFADAWAEFLPVVQDWIEVVERHGP
                     DAIADTYLEVLDGRADPSVAFVLSMD"
     gene            6271..7461
                     /locus_tag="VLR27_13900"
     CDS             6271..7461
                     /locus_tag="VLR27_13900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSP04599.1"
                     /translation="MSPTTRQHDVVVVGSRCAGAATAMLLAQQGVDVVTVDRARFPSD
                     TLSTHSLARGGVVQLHRWGLLDEVLASGAPAIREVSFHIGDTVIRKDIKAAAGVDHLV
                     APRRHILDDILLRAAERAGAEVRTGVTVTDVTRDATGRVAGIVGRGPDGEPVELGARF
                     VVGADGLRSRVARAVGAPVTDQRHADGACHYTYVADLDFQGTEFHLSADGFAGLFPTH
                     CGEANVWVSTSVDRAEGLRGGADRNRAFRSLVAGISPAFAERLDVARQTAPVRGFSGM
                     PNLLRKPVGDGWALVGDASYFRDAITGHGMTDAFRDAEHLARTIGAVLAGDVDEADAM
                     ARYHARRDEMVSDLFEITCALAQFPAPDRFSELQKQLSRCIEAEAEALAALPPIRPVG
                     AFAA"
     gene            7478..7609
                     /locus_tag="VLR27_13905"
     CDS             7478..7609
                     /locus_tag="VLR27_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04600.1"
                     /translation="MTLITIELADAVLAERRRQALGGPRPSPIRRRRRMRRGRLPVT"
     gene            7663..8238
                     /locus_tag="VLR27_13910"
     CDS             7663..8238
                     /locus_tag="VLR27_13910"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSP04601.1"
                     /translation="MSITESNTRPARNGVDTPTLFATLDAVKQAPAAAKFQFRAHNEW
                     QSGTHSRSTIEDFFGVGEERSHERTFVFEADHPAVLVGNDNGPTPVEFLLHGLASCLT
                     AGLANIAAARGVTLHQVRSTVSGDIDLNGILGLDPEVRNGYQQITVDMVVEGDAPPEV
                     LASLVEQSKQRSAVYDVITSSVPVAINVTTG"
     gene            8310..9044
                     /locus_tag="VLR27_13915"
     CDS             8310..9044
                     /locus_tag="VLR27_13915"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HSP04602.1"
                     /translation="MTVTAPTATADRNTTTASSERGPGLVAGARAMLPWLVGVVPFGL
                     MVGITVGSSSIDHGAGLATGLTIYAGSAQLAAISLLEQGAAPAVAVATVLAINARLVF
                     YSASMAPRWQGTSAWFRAAASYLLIDPSYAVGAATYDRHPGRRGHLHYVGAAVVLWTA
                     WQASIATGYLAGAGVPEGLQLEHAIPLFLLAELGHSVRGRPALTAAVVGASVAVAGSG
                     LPLHAGPLLGIAAGIAAAGLVEERRP"
     gene            9041..9361
                     /locus_tag="VLR27_13920"
     CDS             9041..9361
                     /locus_tag="VLR27_13920"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HSP04603.1"
                     /translation="MTAWTVVLLAGLGSYLFRVSIVFAIDRLRAPAGLERMAGYVVPA
                     AFAGLAATAVIEPVAAGGGDGLAVGSAALVTLVLAGRGRPASAAFAGGLLTLWAVSAL
                     VAIT"
     gene            9371..10150
                     /locus_tag="VLR27_13925"
     CDS             9371..10150
                     /locus_tag="VLR27_13925"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSP04604.1"
                     /translation="MTTRTTSPATITDHHDARGLGTVLGVWAHPDDEAYLSAGLMALA
                     RAAGNHVTCVTATLGEHGTEDARTWPPGRLARTRRMEITASMAILGVTDHRILGYEDG
                     TLPEQPAEPAVAAISAVIEEVRPDTIVTFGPDGMTGHPDHQTVSAWTTTAWEQTGRGR
                     LLHATTTDAFAEEHADLHARIPVFGPGLPLRTPAAEVALAVDLDEELLDTKLAALRAQ
                     ATQVGPLLPLIGEQAFRCWWRRETFRAPDPTRAARALTLIS"
     gene            10224..13379
                     /locus_tag="VLR27_13930"
     CDS             10224..13379
                     /locus_tag="VLR27_13930"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSP04605.1"
                     /translation="MKTTRITVLGTFAVERDGVVAPEDGWRRRQAASLVKLLALAPGR
                     RAHREQVVDALWPDHTPDAVAPKLHKATYYARRALGDNDAVVTVGDVLTLLPDDDVVV
                     DAVAFEEAAVGALRCDDQLAMAAALDDHPGDLLPDDPYEEWLGPPRERLAALRADLFR
                     RLGRWEDLVAVDPTDEAAHVALMQALRERGDVHGALRQYERLERALSTELGCTPGPEA
                     SALRDQLLELGSVAASTPADRALELVGREGALASLVALLDDAEQGRGRTAFVRGPVGS
                     GKTALLDALARHAGERGCLVGRGAAARVEGSWPYAPVLEALGELCRNDRSLLEDLDPA
                     YRAELDRALLGSDLHWSGEGGHQRLFVAAAELLRLAGREQCVVLVLDDLHDADEASTR
                     LLHYLARGLTDEPVLLVLAARDGAATSPLLSVQSSLVGRGAATDVQLDRLDEERARRL
                     VAVAAPGLDGEAADRVIAVADGSPFALAELARQAAAGAAPVDTVAEAILRALTPEDRR
                     VLERAALLGSSFDTDEFVAVSDLPDDEAFTHLDHALAARMLEHTGARYRFRHSLVRDA
                     LLAGVPPHRRLAIHRLTAERLASLGASPARIGHHLLEGGDTEAAVPHLLAAADRAAAV
                     GAYRDAYALVERIQDHAVGEHRAQALALRADLLFALGDPTAPAAYRQALPLASGDQAR
                     LLRARLARAATVAGDIDTALAAIDGLDTDGGPTDPDVLLARANVAYFAGDHAAAWDLT
                     QQARTRILSGDQTWQVLDLIALEGLLAHHRGEWFDRMRTELVRTVQDPEIALAIFDSY
                     LCPAEYLLYGPTPYEDVLQLAERLHATALRSGALRAVAFAAALAGEAALLAGDLERAR
                     RELEESMALHREIGARSGEAHSLQRLAEVELAEGDRAAARALLQRALPLARWSTISLH
                     LLQRIYGTMMRAADDPDAALAVIERAEATLGTEDLCPFCAVMLAVPAATASAQADDLD
                     RADLYLAIAEQSGALWEGTSWRAAIVEARAHRAAAAGDPEEAAALMAEARDLFEVAGQ
                     PLDTARCDQAPDASGAAVPLG"
     gene            13436..13822
                     /locus_tag="VLR27_13935"
     CDS             13436..13822
                     /locus_tag="VLR27_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSP04606.1"
                     /translation="MTRPDHHEVVDATVATEDTMTPQRVPVRMYEAPEALVVVAPMCA
                     VQRSDVHIEKRGTHLRFWAHLRSAAPREYLLDEWDFGGYERELEVPEGYGAGIEATLV
                     NGQLVIRLLKGADVGEMRAQPEGAEI"
     gene            complement(13836..17324)
                     /locus_tag="VLR27_13940"
     CDS             complement(13836..17324)
                     /locus_tag="VLR27_13940"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HSP04607.1"
                     /translation="MRISVLGPMEAHRSDGGEALPLGGLRQRAVLARLAVAGGDTVPV
                     DRMVDDLWDGDPPPSAVNTLQSYVSNLRRELRTGDDVIVERSGPGYRLSSAVELVSQR
                     FEQLVAVAADAPVGERVTLLDQALALWRGPALVELADQAWAEAEAARLDELRLSAIEA
                     RYQALLDTGQHQRVVGDLERAVAEHPLREGLTAQLVLALYRSGRQADALRAYERTRAL
                     LAEELGLDPMPELVALADRVLVQDPELAAPPIDAAPTPHEPAPTAAAAARTAIDAPLP
                     RAATVGHEQHLIGRDDELARLGRALGTLRDGDVRVAIVEGEAGVGKTSLVSAAVADVH
                     AAGGLVLWGRCSAEHLVAYQPVLDAVRAFERSLDREAAAVLGDRHPVLGHLLGRTDAP
                     VTERDAYELYESIAALLRDLASDRPLVLVVDDLHWVDRSSLALLGHLVTQGRVPGLGL
                     IGTVRRPAGRPTVDLDRVVADLRRAGSTDVIDLAGIDASSVAELLDRCGRPVDAAVAD
                     AVHARTGGNPLFVEALSSQADDLTATDPRQLPHTVRETLDLRAAALDADDLHLLITAA
                     AIGDPVDLHVLAEVCECGMDRMLDVVDVGVAAGLLVEDPEVIGRVSFTHALARDALIA
                     RSTRNREAQLHLRIADVLAGRPEAVPEAVLAQHLRAAGALCPPERAATASVAAAREAH
                     ELLAPDEARTWARRALAQLDAAPSPVEGADGLRWTARLIISQACRHLGDRDEASEIGA
                     ALLEQARHSGDPQRITVAAEEAVLGTANVGFSFGFVDEPLLALLDEALTSIRSGEGEH
                     PAEEATLLAWSSIALAGGPPGARQHDLAAKAAKAAAASSGCPDVRAISALALRLAQPG
                     PAAVEERLDLHDEMLAAAVAAKWTDLEVLGHVLGVTDLLEAGRVAESRALLEVLRERL
                     APHHRPGFDTYLHFLDACYALLDGEVDRAEERSNRGLADGGRAHGTNATQAWAAEQFM
                     IGWERGQLAGFVDITASMVEDFPEVASWRPAHAAALVAAGDPERGLEVVRPLVEGDRL
                     VVPPDLLWSTTLAMLGDVVWAVDDPHLGRIVADALAPVQNCVAVTAMGALSLGHLIRQ
                     RGLALAVAGDLAGADAALALAVERADACGFRPWAARARVERATVLERQGLDGTALRSE
                     GEAQAAELGTALTLTG"
     gene            complement(17334..18164)
                     /locus_tag="VLR27_13945"
     CDS             complement(17334..18164)
                     /locus_tag="VLR27_13945"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSP04608.1"
                     /translation="MTSGPLHLAGDAWLVRGLRSGEQVEGRERPVRLHANAMVLAGEE
                     PVLVDTGAGADRDGYWAQVEAILDPADVRWVFLSHDDADHRGNLGDVLDRCPAATLVA
                     GPRLIHRLTLDRELPAERCRWVEDGDVFDAGDRPLLAVRPPAYDLPTTRGLFDATTGV
                     YWGADCFGTPVPADITDVAELDDDVWVDGFTAFHLLLSPWIRDVDPIRWRQAVGRVAS
                     LGATTIVSAHGPVIRSPQVGRALDLAFGLAGASMPAAADRFTALPSWRNHVPATTDRI
                     "
     gene            complement(18161..18913)
                     /locus_tag="VLR27_13950"
     CDS             complement(18161..18913)
                     /locus_tag="VLR27_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04609.1"
                     /translation="MITITPTTGPLGTPAGSSRPDAHSHHAADPALDGTRATWPLAGA
                     VAGIASLVAAMAGMATITEEQYLEGVGVIDDLERGGYHVSFVIGLIGVAALFIAAAGW
                     RRWAHHRAADDLAAGTIATALSATATVNIIGYSMAGALALYLDGGMDGGTMSDESLFV
                     NFAYLDFGTLFGWWGATLAAGCVAFLAFRRRILPRWMGITSVVAIAIPLGFAVFTSLP
                     GMPGFIMPLWLVAISVGMVFSRTAGASVGVPR"
     gene            19084..19704
                     /locus_tag="VLR27_13955"
     CDS             19084..19704
                     /locus_tag="VLR27_13955"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP04610.1"
                     /translation="MRDRRAEQRERTRDEIVAAAWERCRAEGLAGLSLRDLAAAVGMR
                     APSLYSYFDSKLAIYDAMFAEGCQQFLDEVAALPRTGDTMTDLKTSLRHFVEFCASDH
                     VRHQLLFQRTIPGFEPSPESYAISVQGLAVLAGWFRELGYDDPADLDLATAIGSGLAS
                     QQISNDPGGDRWLRLVDDAVEMLVAHLERKTETDTETKTKTSRGEP"
     gene            19701..20447
                     /locus_tag="VLR27_13960"
     CDS             19701..20447
                     /locus_tag="VLR27_13960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HSP04611.1"
                     /translation="MMQITRVEEVPTIARPESIRLAETETARMVALLRSLDDEGWAAP
                     TDCPLWDVRAMAGHVLGMVETFSSLRRFARDMRAGGKAAGDGPFIDGLTAVQVQAHAG
                     LSRQELIDRLEVRGPVQAAWRGSRRLMRQIRTTEDGPDGPETWKAGYLFDIILTRDTW
                     MHRVDITRATGTPMELTPDHDGRIVADVVAEWARRHGQPFTLRLAGPAGGAWASGSGG
                     EDIELDAVEFCRILADRAEGTGLLTQRVPF"
     gene            complement(20472..>20996)
                     /locus_tag="VLR27_13965"
     CDS             complement(20472..>20996)
                     /locus_tag="VLR27_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04612.1"
                     /translation="DSGIGPVSPPGSFVRVPVEQAAAELAAILDAEGVGSLVADGPEG
                     IYGHPDHIHAHDVSTRAAELVGIATRYEMTVDREHLHFVDVHVVETATEAVTETWHGH
                     TGTYGHTTVEIDLLVDVRTHIDAKRAAFAAHASQLPEHSETMRLDAEAFAAVYGIEWF
                     NRRGPAGPLDDLVG"
BASE COUNT         2757 a         7903 c         7617 g         2719 t
ORIGIN      
        1 gacctcgagc agcacctggt gcggcggcgt gttcgggtac gggtcgctcg tcatgtggtg
       61 ggcgtagccg ttggcctcgg cccggtccca gagcatctgc agcagggcga acacgagctg
      121 ctggccgacc ttgtcggggt acgagttgta gttgaacgag gcgtaggcgg ggatgtcggg
      181 ttccgtcggg tcgatgaagt cgccctccca gtcgaccgag cggttcagca gcgtcgagta
      241 gttcatgccc ggcacgccga gcacggcctt ggtgaagtcc ggcgccagcg cggtgagggc
      301 gccgcccatg atcccgccct gggagttgcc gtcgtagaac agctcgctgg tgtcgagcag
      361 cgaccggccc tcggcgtcct ggaaggcggg gtgcgtgctg acgccgtcgg ggtggatcag
      421 cgcccggccg acgtacagga agccgaggaa cccctgctgg gcccggtcgg ccagcgacgg
      481 gaagaacgag atgtcgtgga ggatggcggc cacgttgacg atgtcctcgg tggccatgcc
      541 gatccagtcg accccgcaca tggcatggcc gacgcggcgc aggtcggcgg tcgagccacc
      601 ctcggtctcc cctcggccgc ccagcaggcc gtggccgtag agggtgggct tggccccgcc
      661 ggcgacgccg acgggcagct tgcacgtgaa ctccgcctcc atcgtcggca ccagcgggtt
      721 ctggatcggc gtgtccatcg gcccgggcgt cggcgacgcg tacacgaagc gcgactgtgg
      781 gagcacgacc gggagctcac cagccagttc gatcagctcg tcctgggggg tggtcaggta
      841 gttcgggacg gtgacggagc cgtgcacggt gcgggtgacg ttgcccgccg cgtcgacctc
      901 atcgacgtgc tcgctgatcg tcatcgccgg cgcagcaccg gccgggacga gatccgccag
      961 gtccgtgtcg ccgagctggg cgaaggcgtc gtcgcggatg gcgagcgccc tgccggccag
     1021 gttcggcgcc gaggcgacgt ggaagtccca ggcgaggtgg aggtcgtcga ccgccacgcc
     1081 ggacgcatcc agacgggtga agatgtcctg gtaccgaccg gcccggtcgg ccgggtcgat
     1141 gtcggccgcc ggcgggaact tcggcttctt gggcttcccc ttggggttgc gcggcggctc
     1201 gtaggcgtcg cgcagggcgg tgaaggcacc ggtcggggcc agctcggtgc cgtcggccgc
     1261 acgcaggtcc cggagggcga cgatgtagcg gcgcccctcc tcgaggttcg tgaggggacg
     1321 gatgatgagc gtgcggtcgt ggccctcggc cagcgtgacc tcgtgctcgt cgagctcggc
     1381 ccagtagggg tggcgctcgc cggtgtcggc gtccagcagg acgatggggg cgtcgggcgc
     1441 catggacatc gccggggcct cgaccacggc atcgggcagc gcgtcgcggg gggcgaaggg
     1501 gtcgcccgcc gtcgtcgagc cggcgccgga ctccggttcg gggtcgagcc cgaacgtggt
     1561 cgtcaggtcg aggccgggta cgaacgtgag gatcatggac ccgggcgaga aaccgtcgtt
     1621 gcggttccac tccgtcgggt ccatgggctt ggcgccgaac gcccggggcg tggccagcgg
     1681 cgacaggtcg acccgtcggc cggtgggcgt ggtggcgtcc agcgtcgtga actggtcgtt
     1741 ggggaacggc agcaggcaga ggcgggtgtc gaggtcgtcg cacgccccgg cggccgtggc
     1801 cacctcgtcg gcgaccaggt ccgccggcgt gcgctggtct tcagcgtggg cgctcggcag
     1861 cacggcgaag gtggtggcgg ccaggccggc caggcccagc gcgacggtgg cgcggatgtg
     1921 acgacgacgc atggagcgat cccccggctg tgggccggga cccgccccgg cgctgctccg
     1981 gtcttcgacg gcagcggctg gtgttcctgc cggtaacccc gttctggcag tcgtggtgtc
     2041 gcggctacgg cgacgccggc cgcaccccgt aggccttcat cgcaccggcg accatcgact
     2101 cctcgctgac ctcgtccgcg tggaacgtgt acgacggcgc cacgatcggc gccaccgcct
     2161 cgaccgtcag ctcgaacacg ccccgcagcg cgaaccggtc gccgaccatc acccgcagcg
     2221 gtccggcgac cgcatcgaac cgctcgtcgt ccggagcgtg caggtggagc gcgcccgtga
     2281 gcttgaaccc gcgctggcgg aacacgtcca cgacgctgaa gcagccctgc gggttggcct
     2341 tcgcgttccg ggccgagcgc ggcgaggcga tgtgggcgat gacgacgcgg tcgccaccga
     2401 ggtgtgcgaa cacctccttg ggcgacacgt tcggcatgcc gtgctcgtcg gccgtcgcca
     2461 accagccgag gacggcggca tcgagcgcag cgacgacctg ctcgtcgagc tcaggcacgg
     2521 tccgctccca gccgtcgctc caggtgacga gccgccgagg cgtcgccggg gccacctcgt
     2581 gccagcagcc gctcatggac ggcacggcgg tgcgcgtcgt cgacgttggc gccgtacttg
     2641 aacttcgccc ggacgtcgtc gatcgggatc cgcaggcccc ggatcgctcg gagctgggcg
     2701 ccgtgctcga gggggtcgac cgcatccacc tccggctgga ggtcgccgag ctggcgccgc
     2761 aggaccccgg cgatctcgtc cgggtcgtcg accacgacgg cgtcgccggt gatctgcacc
     2821 gcggcgtagt aggtggtcgg gatccctcgc cgagggtcct cgtcgccgat ggccttccac
     2881 tcggacggca cgtaggccca gtcgccggcc acgctcagga cgacggtcgg gttctcggcg
     2941 atcgcctccc agatcgggtt ggggcgggcc aggtggagca cgacctcgtc ggtgtcgagg
     3001 acgaactgcg tgggcacgat gatcgggacg gtgcggtccc gcccgccggc gacgaggtgc
     3061 ccgaaggcgt tggcggtgac gaaggcacgc cactccgcct cgtcgatcgg gcgatcccag
     3121 tcgcggatga gcacgtcgcc atcttggtca tcccgagtcc cacgtcgcag cgacttctcc
     3181 cccacctatg gtccggcccc gtgggggacg accgctggaa ccacaacatc cacctgcacc
     3241 gcgtcgtgct ggacgcggtg ccgccgggtg cgcagcgggc gctcgacgtc ggctgcggcg
     3301 agggcatgct cagccgcgac ctgcacgaga cgatcccgga ggtggtcggc ctcgacctcc
     3361 acgggccgag cctcgacctg gcccgctccc agtccgaggg cgatggcatc acctacgtgc
     3421 tcggcgacgt cctcgaccac cccttcgagc tcgcgtcgtt cgacgtggtc gcgtcgatcg
     3481 ccgccctgca ccacatggac accgtcgcgg gtctcgaggc gatgcgcgac ctcctgcgcc
     3541 ccggtggcgt gctggtcgtc gtgggcctgg cccgcacgcg cttcccgcgc gacctgggct
     3601 ggcaggtggc cggcgcggtc agcacccgct ggctgaagct gagcccgaag cgcacgtact
     3661 gggagcactc ggcacccatc gtgtggccgc cgccggagac ctatgccggc atgcgcacca
     3721 ttgccgagcg cgtcctgccc ggcgtggcgt accggcgtca cgtcctgtgg cgctactccc
     3781 tggtctggac caagcccgcc acgcgctgac cgagctcaga gccggtcggt ggggtacccg
     3841 gcgtaggact ccttgaacac gccggagtgc agcagcacgt gggcgaccag gtcgccggac
     3901 tcggcgtcgg tcacgtcggt gcggacccac gacgactcgg tgcggcggct ctgcccgagg
     3961 gcgaccacct cgcgctccag gacgtactcg tgccccacga agatcgggcc ggcgacgagc
     4021 ctcacctcga ggtccaggaa cagccccacc gacgggccgc gcacggggaa gtcggcgccg
     4081 accttctggg tgaggacgct gaccatctcc atgggcagga tcggccggcc ccacgggttg
     4141 cgctccgtcc ggtaccagtc gctgggctcg gtgatggcgt ccagcttctg ctcgagcgag
     4201 aacgggtaca ggtcgccgtt cggcgtcttc aggtcgaccg acgacgtcac cggaccggag
     4261 cggtcgccga cagcgagctg gtccacgatg aacagctcgc ccgggtcgcc gagcttcgcc
     4321 cgccgctcgt cgagggcggt cgggacgtcg ggggcggccg tgatcgagcc ggcgagcacc
     4381 gggctgccgt cggccttcgc cgccgagatg tcggcccgcg tggagctggt gcgggtcgcg
     4441 cgcgccgtca cgccctcccc ctccacgacc atcgtgcgga agtgggcgct gatgcacccg
     4501 tgctcgaacc aggcgtcgcc ccagaggagg tgggcgagcg ggtcgaactg ggagaagtgg
     4561 gtcggcccct cgatcggtgc gccggacagc ccgagcgagt cggcggtgga ctcgtcgtgc
     4621 aggctggcgt ggccgccgta ggtctgctcg gcgagcatct gtcgcggtgc gcgctcggga
     4681 ccctcgatgg tgacgggtgc gtcgaacggt cgttgctgca tcggtgccac ctccgtcggc
     4741 cggcgccctt cgccgcctcg acgacgtgtc tagacgacct cggcgccggc cgcgagcacg
     4801 tcgtcatcga tggtgaaggc ggcgagcgct ccgccggatg ctgccagcac ggctgtgatg
     4861 accacggcga cgcggaagcc gtccgtgagc gcggccgggt cgccgtagtc cctgccggag
     4921 atgcccgcgg cgaccgggag caccgccacg gcgagcaggc cggccgccct ggcccccgcg
     4981 ttgttcacgc cggaggcgac gccggcgtgg cggatgtcgg ccgcggcgag acgatccgct
     5041 caccggcatc cgccgggggt cgaggcgtcg gggacccgca ccggcgcaac gctacgaccg
     5101 cggtcgtagg gtccgcccat ggacctcgag gtgaagcgcg acgacctgca cgagacccgc
     5161 gtggtcgact ccccgccggc gccgctggag gagggtgagg cccggctggc cgtgcgccgg
     5221 ttcgggttca gcgccaacaa cgtgacctac gccgtgttcg gtgacctcat gggctactgg
     5281 accttcttcc cggcggcacc cgacggcgag ggctggggcc gcatcccggt gtggggcttc
     5341 gccgaggtcg tggaggcgaa cggctccggc ctcgacgacg gcgagctcgt ctacggctac
     5401 ctgcccatgg gcaccgagct ggttgtcacg cccacccgcg tcggcgacca cggcttcacc
     5461 gacgcctccg cccaccgcgc cggcctgccg ccggcgtaca acggctacac ccgctgcgcc
     5521 accgacccgg tctacgaccc ggccaccgag ccgctccaga tgcttcttcg gcccctgttc
     5581 atgaccgact tcgtcctcga cgactggctg gccgacaacg ccttcttcgg cgccggggac
     5641 ggcacggtcg tgctctcgag cgcgtcgtcc aagacgtcgt tcggcctcgc acacctgctg
     5701 gctcgccggg cggaccggcc ccaggtggtc ggcctcacgt ccgcaggcaa cgtggccttc
     5761 gtcgagggtc tcggcgtcta cgaccgggtg atcacctacg acgacgtcgc cacgctccct
     5821 gccgacggcc cgtcggtcta cgtcgacatg gccggcgacg ccggcgtgcg caaggcggtc
     5881 catgagcaga tgggcgggct cgcggccagc gtgcaggtcg gcggcaccca ctgggagcag
     5941 gtcggcggtg gcggcgacct ccccggtccg gcgccagcgc tgttcttcgc ccccgaccag
     6001 atcgtgaagc gccgggagga ctggggcccc ggcggggtcg agtcccgctt cgccgacgcg
     6061 tgggccgagt tcctccccgt ggtccaggac tggatcgagg tcgtcgagcg ccacggtccc
     6121 gacgccatcg ccgacaccta cctcgaggtg ctcgacggcc gggccgaccc gtcggtggca
     6181 ttcgtgctgt cgatggactg agggagcgac cagcccgccg gggaagccgg gggaagcgcc
     6241 ggggaagccg gtcgccggat ggtgggcgac atgagcccca ccacccgaca gcacgacgtg
     6301 gtcgtcgtcg gcagccgctg cgccggcgcc gccaccgcca tgctcctcgc ccagcagggc
     6361 gtcgacgtcg tcacggtcga ccgcgcccgg ttcccgagcg acaccctctc cacccactcc
     6421 ctcgcccggg gtggcgtggt gcagctgcac cgctggggcc tcctcgacga ggtcctggcc
     6481 agcggcgccc cggccatccg ggaggtgtcg ttccacatcg gcgacaccgt catccgcaag
     6541 gacatcaagg ccgccgccgg cgtcgaccac ctggtggccc cccgccgcca catcctcgac
     6601 gacatcctcc tccgggcggc cgagcgcgcc ggcgccgagg tccgcacggg cgtcaccgtc
     6661 accgacgtga cccgggacgc caccgggcgg gtcgccggca tcgtcggccg gggcccggac
     6721 ggagaacccg tcgagctggg cgcccggttc gtggtcggcg ccgacggcct ccgctcccgg
     6781 gtcgcccgag ccgtgggcgc tccggtgacc gaccagcgcc acgccgacgg cgcctgccac
     6841 tacacctacg tcgccgacct ggacttccag ggcaccgagt tccacctcag cgccgacggc
     6901 ttcgccggcc tgttccccac ccactgcggc gaagccaacg tgtgggtgtc cacctccgtc
     6961 gaccgggccg aaggcctgcg gggcggcgcc gatcgcaacc gtgcgttccg gtcgctggtc
     7021 gccgggatca gcccggcctt cgccgaacgc ctcgacgtcg cccgccagac cgccccggtc
     7081 cgtggcttct cgggcatgcc gaacctgctg cgcaagccgg tgggcgacgg gtgggcgctg
     7141 gtcggcgacg cctcctactt ccgcgacgcc atcaccgggc acggcatgac cgatgccttc
     7201 cgcgacgccg agcacctggc ccgcaccatc ggagccgtcc tcgccggtga tgtcgacgag
     7261 gccgacgcca tggcccgcta ccacgcccgc cgcgacgaga tggtctcgga cctcttcgag
     7321 atcacctgcg ccctggcgca gttcccggcc ccggaccgct tctccgagct ccagaagcag
     7381 ctgagccgct gcatcgaggc cgaagccgaa gcgctggccg ccctcccgcc catccgaccc
     7441 gtcggcgcct tcgccgcctg atccccagga gaccgccatg accctcatca ccatcgagct
     7501 cgccgacgcc gtgctcgccg aacggcgccg ccaggcgctc ggcggacccc gcccgtcgcc
     7561 gatccgccgc cggcgccgaa tgcgccgcgg acgcctgccc gtcacgtgac ggcacggccc
     7621 agaccaccca ccaaccacac caacaacaca aggagcaatc ccatgagcat caccgagagc
     7681 aacacccgtc cggcccgcaa cggcgtcgac acgccgaccc tgttcgccac cctcgacgcc
     7741 gtcaagcagg cgccggccgc ggcgaagttc cagttccggg cccacaacga gtggcagtcc
     7801 ggcacgcaca gccgcagcac catcgaggac ttcttcgggg tgggcgagga gcgcagccac
     7861 gagcgcacgt tcgtgttcga ggccgaccac ccggccgtcc tcgtcggcaa cgacaacggc
     7921 cccacgccgg tggagttcct cctgcacggc ctcgccagct gcctcacggc cggcctggcc
     7981 aacatcgccg ccgctcgcgg cgtgaccctg caccaggtcc gctcgaccgt ctcgggcgac
     8041 atcgacctca acggcatcct cgggctcgac cccgaggtcc gcaacggcta ccagcagatc
     8101 accgtcgaca tggtcgtcga gggtgacgcc ccgcccgagg tgctggcctc cctggtggag
     8161 cagtccaagc agcgctccgc cgtctacgac gtgatcacca gctcggtgcc cgtcgccatc
     8221 aacgtcacca ccgggtgaca ccccgccgcc gtggccggtc cggacctccc cggaccggcc
     8281 acgcaggcac gctccgagga gccctcccga tgaccgtcac cgcacccacc gccaccgccg
     8341 accgcaacac cacgactgcc agttccgaaa ggggtcccgg cctcgtcgcc ggcgcacggg
     8401 cgatgctgcc gtggctggtc ggcgtggtgc ccttcggcct catggtcggc atcaccgtcg
     8461 gctcgagctc catcgaccac ggcgccggcc tcgccaccgg cctcaccatc tacgccggca
     8521 gcgcccagct cgccgccatc agcttgctcg agcagggcgc cgcccctgcc gtggccgtcg
     8581 ccacggtgct cgccatcaac gcccgcctcg tcttctacag cgcctcgatg gcgccccgct
     8641 ggcagggcac cagcgcctgg ttccgcgccg ccgcctccta cctgctcatc gacccctcct
     8701 acgccgtcgg cgccgcgacc tacgaccggc acccgggccg gcgtgggcac ctccactacg
     8761 tcggcgccgc ggtcgtcctc tggaccgcct ggcaggcgtc catcgccacc ggctacctgg
     8821 ccggagcggg cgtgcccgag ggcctgcagc tcgagcacgc gatcccgctg ttcctcctgg
     8881 ccgagctcgg gcactccgtg cggggacggc cggcgctcac cgctgcggtc gtcggggcct
     8941 ccgtcgccgt cgccgggagc ggcctcccac tccacgccgg gccgctgctc ggcatcgcgg
     9001 ccggcatcgc cgcggcgggt ctcgtcgagg agcgccggcc atgaccgcct ggacggtcgt
     9061 cctcctcgcc ggcctcggct cctacctgtt ccgggtcagc atcgtcttcg ccatcgatcg
     9121 cctgcgcgca cccgccgggc tggagcggat ggccggctac gtcgtgcccg ctgcgttcgc
     9181 cggtctcgcc gccacggcgg tgatcgagcc ggtcgcggcc ggcggcggcg acggcctcgc
     9241 cgtcgggtcc gccgccctcg tcaccctcgt cctggccggc cgcggacgac cggcctctgc
     9301 cgccttcgcc ggcggcctcc tcaccctctg ggccgtcagc gccctcgtcg ccatcaccta
     9361 ggagcgaacc atgaccacca ggaccacgag ccccgccacc atcaccgacc accacgatgc
     9421 ccgtggcctc ggcaccgtcc tcggcgtgtg ggcccacccc gacgacgagg cctacctctc
     9481 cgccgggctc atggccctcg cccgggccgc cgggaaccac gtcacctgcg tgacggcgac
     9541 cctcggcgag cacggcaccg aggacgcacg cacctggccg ccggggcgcc tggcccggac
     9601 ccgtcgcatg gagatcaccg cctccatggc gatcctgggc gtcaccgacc accgcatcct
     9661 cggctacgag gacggcaccc tccccgaaca accggccgaa ccggccgtcg ccgccatcag
     9721 cgccgtgatc gaggaggtcc gacccgacac catcgtcacc ttcggccccg acggcatgac
     9781 gggccacccg gatcaccaga ccgtctcggc gtggaccacc acggcctggg agcagaccgg
     9841 tcgcggtcga ctgctgcacg ccaccaccac cgacgccttc gctgaggagc acgccgatct
     9901 gcacgcgcgc atccccgtgt tcggccccgg cctcccgctg cgcacgccgg ctgccgaggt
     9961 ggcgttggcc gtcgacctcg acgaggagct gctcgacacc aagctcgccg ccctccgcgc
    10021 ccaggccacc caggtcggac ccctgctgcc gctgatcggc gagcaggcgt tccggtgctg
    10081 gtggcggcgg gagaccttcc gggcccccga ccccacccgg gcagcacgtg cgctgaccct
    10141 gatctcctga ccaggagcag gacaaagctg ctgcagcccg ccgggaggtg ggggtaccgt
    10201 gccccctgca cagggagcag atcgtgaaga cgacgcgcat caccgtgctg gggaccttcg
    10261 ccgtggagcg cgacggcgtc gtcgcgcccg aggacgggtg gcgccgtcgc caggctgcct
    10321 cgctcgtcaa gctgctcgcg ctcgcacccg gccgccgggc gcaccgcgag caggtggtgg
    10381 atgcgctctg gcccgaccac accccggacg ccgtcgctcc gaagctgcac aaggccacct
    10441 actacgcccg gcgggcgctc ggtgacaacg acgcggtcgt caccgtcggc gacgtgctca
    10501 ccctgctgcc cgacgacgac gtggtcgtcg acgccgtcgc cttcgaggag gccgcggtcg
    10561 gagcgctccg ctgcgacgac cagctggcga tggctgcagc cctggacgac caccccggcg
    10621 acctgctgcc cgacgacccg tacgaggagt ggctcgggcc cccgcgggag cggctggccg
    10681 cgctgcgcgc cgacctgttc cggcggctcg ggcggtggga ggacctcgtc gccgtcgacc
    10741 ccaccgacga ggcggcgcac gtcgcgctca tgcaggcgct gcgcgagcgg ggcgacgtgc
    10801 acggcgcgct gcgccagtac gagcggctcg agcgggcgct gtcgaccgag ctcggctgca
    10861 ccccgggacc ggaggcgtcc gcgctgcgcg accagctcct cgagctcggc tcggtggcgg
    10921 cgagcacgcc ggcggaccgt gccctcgagc tggtgggccg ggagggcgcc ctcgcctcgc
    10981 tcgtggccct gctcgacgac gccgagcagg gccggggacg caccgcgttc gtgcgcgggc
    11041 ccgtcggttc cggcaagacc gcgctgctcg atgccctcgc ccgtcacgcc ggcgagcgtg
    11101 gctgcctggt cggccggggc gcggccgcgc gggtcgaggg gtcctggccc tacgcaccgg
    11161 tgctcgaggc gctcggcgag ctgtgccgga acgaccgctc gctgctggag gacctcgacc
    11221 cggcgtaccg cgccgagctc gaccgggcgt tgctcggctc cgacctgcac tggtcgggcg
    11281 agggcggcca ccagcgcctg ttcgtcgccg ccgccgagct gctccgcctg gccggccggg
    11341 aacagtgcgt ggtgctcgtg ctcgacgacc tccacgacgc cgacgaggcc agcacccgcc
    11401 tgctgcacta cctcgcccgt gggctgacgg acgagccggt gctgctcgtg ctcgccgccc
    11461 gggacggagc agcgacgagc ccgctgctgt cggtgcagtc cagcctcgtc ggacgtggtg
    11521 ccgcgaccga cgtgcagctc gaccggctgg acgaggagcg ggctcgccgg ctcgtcgccg
    11581 tcgccgcccc cggcctggac ggcgaggcgg cggaccgcgt catcgccgtc gccgacggtt
    11641 ccccgttcgc cctcgccgag ctcgcccgcc aggcggccgc cggcgcggcg cccgtggaca
    11701 cggtggccga ggcgatcctg cgcgccctca cccccgagga caggcgggtg ctcgaacggg
    11761 ctgccctgct cgggtcctcg ttcgacaccg atgagttcgt cgccgtcagc gacctgcccg
    11821 acgacgaggc cttcacccac ctggaccacg ccctcgccgc ccggatgctc gagcacaccg
    11881 gcgcccgcta ccggttccgc cacagcctcg tgcgggatgc cctgctcgcc ggggtcccgc
    11941 cgcaccgacg cctcgcgatc caccgcctca ccgctgagcg gctcgcctcg ctcggcgcat
    12001 caccggcccg gatcggtcac cacctcctgg agggcggcga caccgaggca gccgtgcccc
    12061 acctcctcgc cgccgccgac cgggcggcgg ccgtgggcgc ctaccgcgac gcctacgcgc
    12121 tcgtcgagcg catccaggac cacgccgtcg gcgagcaccg ggcccaggcc ctggccctgc
    12181 gcgccgatct gctcttcgcc ctcggcgacc ccaccgcccc ggcggcctac cgccaggcgt
    12241 tgccgctcgc ctccggagac caggcccggt tgctgcgcgc ccgcctggcc cgggccgcca
    12301 ccgtcgccgg tgacatcgac acggccctcg ccgccatcga cgggctcgac accgacggcg
    12361 gccccaccga tcccgacgtg ctcctcgctc gcgccaacgt cgcctacttc gccggcgacc
    12421 acgccgcagc ctgggacctc acccagcagg cccgcacccg gatcctgagc ggcgaccaga
    12481 cctggcaggt gctcgacctg atcgcgctcg aggggctgct cgcccaccac cggggcgagt
    12541 ggttcgaccg catgcgcacc gagctggtcc ggaccgtcca ggatcccgag atcgccctcg
    12601 ccatcttcga cagctacctg tgcccggccg agtacctgct ctacgggccg acgccgtacg
    12661 aggacgtgct gcagctcgcc gagcgcctcc acgccaccgc cctgcggtcc ggtgcgctgc
    12721 gggcggtggc gttcgccgcc gccctggccg gcgaggccgc cctgctcgcg ggcgacctcg
    12781 agcgggcccg ccgcgagctc gaggagtcca tggcgctgca ccgcgagatc ggcgcccgct
    12841 ccggggaggc ccacagcctg cagcgcctcg ccgaggtcga gctggccgag ggggaccggg
    12901 ccgcagcccg tgccctgctg cagcgggccc tgcccctcgc ccgctggagc acgatctcgc
    12961 tccacctgct ccaacgcatc tacgggacga tgatgcgggc ggcggacgac cccgatgcgg
    13021 cgctcgccgt catcgaacgg gccgaggcca ccctcggcac cgaggacctg tgcccgttct
    13081 gcgcagtgat gctggccgtc cctgccgcca ccgccagcgc ccaggccgac gacctggacc
    13141 gggccgacct gtacctcgcc atcgccgagc agtcgggtgc gctgtgggag ggcacgtcct
    13201 ggcgggccgc catcgtcgag gcccgggcgc accgggcagc cgcggcgggc gacccggagg
    13261 aggcggcggc gctcatggcc gaggctcgtg atctgttcga ggtggccggc caaccgctgg
    13321 acacggcccg gtgcgatcag gcgccggacg catccggcgc ggccgtcccg ctcggctgag
    13381 ccccggcggc ggggacccgg ctgacaggac ggcccggcgg gcgtacggtc gggccatgac
    13441 ccgccccgat caccacgagg tcgtggacgc gaccgtcgcc accgaggaca ccatgacgcc
    13501 ccaacgggtg ccggtgcgga tgtacgaggc ccccgaggcg ctcgtcgtcg tcgcgccgat
    13561 gtgcgcggtg cagcgcagcg acgtccacat cgagaagcgc ggcacgcacc tgcggttctg
    13621 ggcccacctg cgcagcgccg caccccgcga gtacctgctc gacgagtggg acttcggtgg
    13681 ctacgagcgc gagctggagg tgcccgaggg ctatggcgcc ggcatcgagg ccacgctggt
    13741 caacggccag ctggtgatcc gcctcctgaa gggcgccgac gtcggcgaga tgcgcgccca
    13801 gcccgagggc gcggagatct gagccccggc cggcgtcagc cggtcagggt gagcgcggtc
    13861 ccgagctcgg ccgcctgggc ctccccctcg gagcgcagtg ccgtcccgtc gaggccctgc
    13921 cgctccagca ccgtcgcccg ctcgacgcgg gcgcgggccg cccaggggcg gaacccgcac
    13981 gcgtcggccc gctcgacggc gagggcgagc gcggcgtcgg cgccggccag gtcgccggcg
    14041 acggcgagcg ccagtccccg ctggcggatc aggtgaccga gggacagcgc gcccatggcg
    14101 gtgacggcga cgcagttctg caccggcgcc agcgcgtcgg ccacgatccg tccgaggtgc
    14161 gggtcgtcca ccgcccacac gacgtcgccg agcatggcca gggtcgtcga ccacagcagg
    14221 tcgggcggga cgaccagccg gtcgccctcg acgaggggac gcaccacctc gagcccgcgc
    14281 tccgggtcac cggcggcgac cagggccgcg gcgtgcgccg gccgccacga ggcgacctcg
    14341 gggaagtcct cgaccatcga tgcggtgatg tcgacgaacc cggcgagctg gccgcgctcc
    14401 cacccgatca tgaactgctc ggcggcccag gcctgcgtgg cgttggtgcc gtgggcccgt
    14461 ccaccgtcgg ccaggccccg gttgctccgc tcctcggccc gatcgacctc accgtccagc
    14521 agcgcgtagc aggcgtcgag gaagtggagg taggtgtcga agccgggccg gtggtggggg
    14581 gccagccgct cgcgcagcac ctcgagcagc gcccgtgact cggcgacccg accggcttcg
    14641 agcaggtcgg tcaccccgag cacgtggccg agcacctcga ggtcggtcca cttggcggcg
    14701 accgcggcgg cgagcatctc gtcgtgcagg tccaggcgct cctcgaccgc agccgggccg
    14761 ggctgggcca accgcagcgc cagcgcgctg atggcccgca cgtcggggca gccggagctg
    14821 gccgcagccg ccttcgccgc cttggccgcc aggtcgtgct gccgggcgcc aggcgggccg
    14881 ccggcgagcg cgatcgagga ccaggccagc agtgtcgcct cctccgccgg gtgctccccc
    14941 tcgcccgacc ggatggacgt cagagcctcg tccagcagtg ccagcagggg ctcgtcgacg
    15001 aagccgaagc tgaagccgac gttcgcggtg ccgaggacgg cctcctcggc ggcgacggtg
    15061 atccgttgcg gatcaccgga gtgtctggcc tgctcgagca gcgcggcccc gatctccgag
    15121 gcttcgtccc ggtctcccag gtgccggcag gcctgcgaga tgatcagccg cgcggtccag
    15181 cggagcccgt cggcgccctc caccggcgag ggtgccgcgt cgagctgggc cagcgcccgg
    15241 cgcgcccagg tgcgcgcctc gtccggcgcg agcagctcgt gggcctcccg tgccgcggcg
    15301 accgacgcgg tggcggcccg ctccggcggg cacagggccc cggccgcccg caggtgctgg
    15361 gccagcacgg cttcgggcac cgcctcgggg cggccggcga gcacgtcggc gatgcgcagg
    15421 tgcagctggg cctcacggtt ccgcgtcgag cgggcgatca gcgcgtcacg cgcgagcgcg
    15481 tgggtgaacg acacccgccc gatcacctcg gggtcctcca ccaggagccc ggcggcgacg
    15541 ccgacgtcga ccacgtcgag catccggtcc atcccgcact cgcagacctc ggcgagcacg
    15601 tggagatcga ccggatcacc gatggcggcc gccgtgatca ggaggtgcag gtcgtcggcg
    15661 tcgagcgcgg cggcccggag gtcgagcgtc tcgcggaccg tgtggggcag ctggcggggg
    15721 tcggtggcgg tgaggtcgtc ggcctggctc gagagcgcct cgacgaagag ggggttcccg
    15781 ccggtgcggg cgtggacggc gtcggccacg gcggcgtcga ccggacgccc gcagcggtcg
    15841 agcagctcgg cgaccgacga cgcgtcgatg ccggccaggt cgatgacgtc ggtcgagccg
    15901 gcgcggcgca ggtcggcgac cacccggtcc aggtcaacgg tcggtcgacc cgccggccgc
    15961 cgcacggtgc cgatcagccc gagacccggc acccgtccct gggtgacgag gtggccgagc
    16021 agggcgaggg atgagcgatc gacccagtgc aggtcgtcga cgacgaggac cagcggtcgg
    16081 tccgaggcca ggtcgcggag cagggcggcg atggactcgt agagctcgta ggcatcgcgc
    16141 tcggtgaccg gggcgtcggt gcgcccgagg aggtgaccca gcaccgggtg gcgatcgccg
    16201 agcacggcag ccgcctcccg gtcgaggctg cgctcgaagg cccggaccgc gtcgaggacc
    16261 ggctggtagg cgacgaggtg ctccgcggag cagcgtcccc acagcaccag gccgccggcg
    16321 gcgtgcacgt ccgccacggc ggccgacacg agcgaggtct tgcccacacc ggcctcgccc
    16381 tcgacgatcg cgacccgcac gtcgccgtcg cgcagcgtcc cgagggcgcg cccgagccgg
    16441 gcgagctcgt catcccggcc gatgaggtgc tgctcgtggc cgacggtcgc tgctcgcggc
    16501 agcggggcgt cgatggccgt gcgggcagcg gccgccgccg tcggggcggg ctcgtgcggc
    16561 gtgggggcgg cgtcgatcgg cggcgcggcg agctcggggt cctgcaccag cacccgatcg
    16621 gccagggcga ccagctcggg catggggtcg agccccagct cctcggccag cagggcccgg
    16681 gtgcgctcgt aggcccggag ggcgtcggcc tggcgaccgg agcggtagag ggcgaggacg
    16741 agctgggcgg tgaggccctc gcgcagcggg tgctcggcca ccgctcgctc caggtcgccc
    16801 accacccgct ggtgctggcc ggtgtcgagc agcgcctggt agcgggcctc gatggccgac
    16861 agccgcagct cgtcgagccg ggccgcctcg gcctcggccc aggcctggtc ggccagctcc
    16921 accagcgccg gcccccgcca cagcgcgagg gcctggtcca ggagggtgac ccgctcgccc
    16981 accggggcgt cggctgcgac cgcgaccagc tgctcgaagc gctgggacac cagctcgacc
    17041 gccgaggaga gccggtagcc gggacccgac cgttcgacga tgacgtcgtc gccggtgcgc
    17101 agctcccggc gcaggttcga gacgtagctc tggagcgtgt tcaccgccga cggtggcggg
    17161 tccccgtccc agaggtcgtc caccatccgg tcgaccggga cggtgtcgcc acccgccacc
    17221 gccaaccggg ccaggacggc acgctggcgc aaccccccga gggggagcgc ctcaccgccg
    17281 tcgctgcgat gggcctccat cgggcccagc accgagatcc gcaccggcgg atcttagatc
    17341 cggtcggtcg tcgccgggac gtgattccgc caggacggca gcgcggtgaa ccggtcggcc
    17401 gcggccggca tcgatgcgcc ggcgaggccg aacgccaggt cgagggcccg gcccacctgc
    17461 gggctgcgga tcaccggccc gtgggcgctg acgatggtgg tggcgccgag gctggccacc
    17521 cgcccgaccg cctggcgcca gcggatcgga tcgacgtcgc ggatccaggg gctcagcagc
    17581 aggtggaagg cggtgaaccc gtcgacccac acgtcatcgt cgagctcggc gacgtcggtg
    17641 atgtcggccg gcaccggcgt cccgaagcag tcggcacccc agtagacgcc ggtggtggcg
    17701 tcgaacaggc cccgggtggt cggcaggtcg taggccggcg gacgcacggc gaggagcggg
    17761 cggtcgccgg cgtcgaagac atcgccgtcc tccacccacc ggcaccgctc ggccgggagc
    17821 tcccggtcga gggtgagccg gtggatgagc cggggcccgg ccaccagcgt ggcggccggg
    17881 caccgatcga gcacgtcgcc caggttcccc cggtgatcgg cgtcgtcgtg gctgaggaag
    17941 acccagcgca cgtcggcggg atcgaggatg gcctcgacct gggcccagta gccgtcccgg
    18001 tccgctccgg cgccggtgtc gaccagcacc ggctcctcgc cggccagcac catggcgttg
    18061 gcgtgcaggc ggacggggcg ctcccgtccc tcgacctgct cgccggaccg gaggccccgg
    18121 accagccagg cgtcaccggc gaggtgcagc gggcccgagg tcatcgcggg acgccgaccg
    18181 aggcacctgc cgtgcgggag aacaccatgc cgacgctgat ggccacgagc cacaggggca
    18241 tgatgaaccc gggcatcccg ggcagggacg tgaacaccgc gaagccgagc gggatcgcga
    18301 tggccacgac gctggtgatg cccatccagc ggggcaggat ccggcgccgg aaggcgagga
    18361 acgccacgca gccggcggcg agcgtcgccc cccaccagcc gaacagggtg ccgaagtcca
    18421 ggtaggcgaa gttcacgaac agcgactcgt ccgacatggt gccgccgtcc atgccgccgt
    18481 cgaggtacag cgccagggcg ccggccatcg agtagccgat gatgttcacc gtcgccgttg
    18541 ccgacagggc ggtggcgatg gtgccggcgg cgaggtcgtc ggcggcccgg tggtgggccc
    18601 agcggcgcca gccagcggcg gcgatgaaca gggcggcgac cccgatgagg ccgatgacga
    18661 aggagacgtg gtagccgcct cgctccaggt cgtcgatcac gccgacgccc tcgaggtact
    18721 gctcctcggt gatggtggcc atgccggcca tggccgcgac caggctggcg atgccggcga
    18781 cggcgccggc gaggggccag gtggcccggg tgccgtcgag cgcggggtcg gcggcgtggt
    18841 ggctgtgggc gtcgggccgg gacgagccgg cgggcgtgcc cagcgggccg gtggtggggg
    18901 tgatggtgat cacgggtggt gctcctgcgg tggggctggt ggttggtgcg atcaccgtcg
    18961 cccgccctcc atggagccct cgtggagcgc gcttggagca ggttccactt gacttccatc
    19021 gaacgccgtt ccattgtacg aacagtgtta ggtgaagcgg ggacggagac gggcaccgcg
    19081 ccggtgcggg accgccgggc ggagcagcgg gagcgcacgc gcgacgagat cgtggcggcg
    19141 gcgtgggagc gatgccgggc ggaggggctg gccgggctgt cgctgcgcga cctcgccgcc
    19201 gccgtcggca tgcgggcccc gtcgctgtac tcctacttcg actccaagct cgccatctac
    19261 gacgccatgt tcgccgaggg ctgccagcag ttcctggacg aggtggcggc gctgccccgc
    19321 accggggaca cgatgaccga cctcaagacg agcctgcgcc acttcgtcga gttctgcgcc
    19381 agcgaccacg tccgccacca gctgctgttc cagcggacca tccccggctt cgagccctcg
    19441 cccgagtcct acgccatctc cgtgcagggg ctcgcagtgc tggccgggtg gttccgcgag
    19501 ctcggctacg acgacccggc cgacctcgac ctggccaccg ccatcggctc cggcctggcg
    19561 tcccagcaga tcagcaacga cccgggaggc gatcgctggc tgcgcctggt cgacgacgcc
    19621 gtcgagatgc tcgtcgccca cctcgaacgc aagaccgaga ccgacaccga gaccaagacc
    19681 aagaccagca ggggggaacc atgatgcaga tcacgagggt cgaggaggtg ccgaccatcg
    19741 ctcggcccga gtcgatccgc ctggccgaga ccgagacggc acggatggtc gccctgctgc
    19801 gctcgctcga cgacgaaggc tgggcagcac caaccgactg cccgctgtgg gacgtgcgag
    19861 ccatggccgg ccacgtgctc ggcatggtcg agacgttctc gtccctgcgc cgcttcgccc
    19921 gcgacatgcg cgccgggggc aaggccgccg gcgacgggcc cttcatcgac ggcctcaccg
    19981 ccgtgcaggt ccaggcccat gccggcctgt cccgccagga gctgatcgac cggctcgagg
    20041 tgcgaggccc ggtccaggcg gcctggcggg gcagccggcg cctcatgcga cagatccgca
    20101 cgaccgagga cggacccgac gggcccgaga cgtggaaggc cggctacctg ttcgacatca
    20161 tcctcacccg ggacacgtgg atgcaccggg tcgacatcac ccgggccacc ggcacgccga
    20221 tggagctcac gccggaccac gacggacgca tcgtcgccga cgtggtcgcc gagtgggctc
    20281 gccgccacgg ccagcccttc accctccgcc tcgccggtcc ggccggcggg gcctgggcat
    20341 ccggctccgg cggcgaggac atcgagctcg acgctgtgga gttctgccgg atcctcgccg
    20401 accgggccga ggggacgggg ctgctcaccc agcgcgtccc gttctgatcc ggggctaccc
    20461 ggcccggctg cctagccgac caggtcgtcc agcgggcccg ccgggccgcg gcggttgaac
    20521 cactcgatgc cgtagaccgc ggcgaacgcc tcggcatcca gccgcatggt ctcggagtgc
    20581 tcgggcagct ggctggcgtg ggcggcgaac gcggcgcgct tggcgtcgat gtgggtgcgc
    20641 acgtcgacga gcaggtcgat ctccaccgtc gtgtgcccgt aggtgccggt gtgcccgtgc
    20701 caggtctcgg tcaccgcctc ggtcgcggtc tcgaccacgt ggacgtcgac gaagtggagg
    20761 tgctcccggt cgacggtcat ctcgtagcgg gtggcgatgc cgaccagctc ggcggcccgg
    20821 gtcgacacgt cgtgggcgtg gatgtgatcg gggtgcccgt agatgccctc gggcccgtcg
    20881 gccacgaggg aaccgacccc ctcggcatcg aggatggcgg ccagctcggc cgccgcctgc
    20941 tccaccggca cccgcacgaa cgatccgggt ggcgacaccg gcccgatccc ggagtc
//
LOCUS       DATISU010000350        10925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_258410, whole genome shotgun sequence.
ACCESSION   DATISU010000350 DATISU010000000
VERSION     DATISU010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10925
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258410"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VLR27_13970"
     CDS             complement(<1..448)
                     /locus_tag="VLR27_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04613.1"
                     /translation="MVLRGKWAQGIEPRNFTWVLQGKLAVCERPGGYGQNHRKVRRQE
                     EIIWLREQGFTMVLSLAASPHNLHNYDEMGMPYRHRPFRRTDDRETFLATLFPELRQL
                     LVDGGKIVLHQDELGDRLAGVMAAYVLWNRLVPGPPQAISIVEQLLH"
     gene            complement(448..1263)
                     /gene="folP"
                     /locus_tag="VLR27_13975"
     CDS             complement(448..1263)
                     /gene="folP"
                     /locus_tag="VLR27_13975"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HSP04614.1"
                     /translation="MRPLVMGVLNVTPDSFSDGGAWADTDAAVRRGRELLAEGADIVD
                     VGGESTRPGAEPVGIDTELARVVPTIEALVGDVAAAGARLSVDTRHAEVARAALAAGA
                     TILNDVSASLEQVAGDADAGWVAMHMLGEPGTMQDSPHYDDVVAEVRAFLVAAEQRGR
                     DAGVDEIWIDPGIGFGKTFEHNWALLGALDQLVATGIPVAVGTSRKGFLGAVLAEADG
                     TDDRPGPDDRLEGSITTACWAATMGAAMIRVHDVRATVAALDAALAAPGPGGT"
     gene            1538..2545
                     /locus_tag="VLR27_13980"
     CDS             1538..2545
                     /locus_tag="VLR27_13980"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSP04615.1"
                     /translation="MRSPIRTRLVAVLAALALALAACGDDDDSGVAASGDPTDTTEAA
                     DDGSTEEGAEEATFPVTVTVDGTDTTIEAEPQAIVSLSPTATEMLFAVGAGEQVIAVD
                     DFSNFPEDAPVTDLSGFDPNVEAIIGYDPDLVILSGDRNDVVSGLAAVDVPTIVLPSA
                     TTIEDTYTQIEVVGAATGHVGDAAEVVANMQSDIDELVAQLPEQDEPLTYYHELDDTL
                     YSVTSDTFIGEIYGLAGLENVADTADGAAEAGGYPQLSAEFLIDADPDLIFLADTKCC
                     GQDASTVAERPGWSEMSAVQQGDVVELDDDIASRWGPRIVDFLEAIVEATADVDAGAA
                     G"
     gene            2556..3680
                     /locus_tag="VLR27_13985"
     CDS             2556..3680
                     /locus_tag="VLR27_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HSP04616.1"
                     /translation="MTTATDGPGITTGPSVAPVEPPDGDDLVGTLPAATDLSPRWLLA
                     GLAAVAVATVVGIATGPVSLPLHRVAVELVDHLPLVSIDSGLSDTQRAIVWEIRTPRV
                     VLGLLVGALLAGSGAGYQGVFRNPLADPYLLGIAAGAGLGATLAIVTGAGDGVGAFDA
                     IPMFAFIGALVAVAMAAALSVRRDGSGGPATLILAGVAVAAFFTAAQTYVQQRHAETL
                     RQVYAWILGRLSTSGWDEVLLLLPYALICGAVLVLAVGALDVLAVGDDEARALGLHPR
                     RIRMAVLVAASLATAAAVAVSGLIGFVGLVVPHGVRLVAGVSNRRVIPLSFLFGGAFL
                     ALADVLARTVQAPAELPIGVITAFVGAPFFLWILRSRSMA"
     gene            3677..4537
                     /locus_tag="VLR27_13990"
     CDS             3677..4537
                     /locus_tag="VLR27_13990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP04617.1"
                     /translation="MSTHPAISWRDVGVELGGSTVVRDVSLDVPTGAWLSLIGPNGAG
                     KTTLVRTLGGTVDHAGDVELLGRDGRGLSVRDRARQLAVVPQHPVIPPGMRVFDYVLL
                     GRAPHQGLRCASSLQDRRRSLAVLQRLGLEDFADRRVDTLSGGERQRVVLARALVQDT
                     PVLVLDEPTAALDVGHQLEVLELIADLRTERELTIVTTVHDLTVAGQFADLVAVLADG
                     RLVAAGTPREVLTPDVIGAHWGVEASCDVDAEDGSVTVTVRRRKEQRRRPATPATTEL
                     APHATEEDHR"
     gene            4534..5112
                     /gene="cobO"
                     /locus_tag="VLR27_13995"
     CDS             4534..5112
                     /gene="cobO"
                     /locus_tag="VLR27_13995"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HSP04618.1"
                     /translation="MSDTPTDAPPTEHQEPPELRRADSLVLVNTGNGKGKSTAAFGTM
                     IRSVARGWPVAVVQFLKSGKWNTGEEKVARDLGVEWFAIGEGFTWDSEDLSRDEAVAR
                     EAWARAASLLESGTHRLVVLDEITYPMNWGWFDTDEVVAAIRDRSPKVNVICTGRDAP
                     EPLLELADTATEMRVLKHAYQSGIRAMKGIDY"
     gene            5113..6645
                     /locus_tag="VLR27_14000"
     CDS             5113..6645
                     /locus_tag="VLR27_14000"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HSP04619.1"
                     /translation="MGAPPHGALLVVGATSGAGKSTVTAGLCRWFARTGATVAPFKAQ
                     NMSNHSAVSVDGGEVGRAQAMQAAAAGVDVESTMNPILLKPSSATGSHVVVLGREVGT
                     TDAAGWGVRTDELRPVVLDALTSLRRRFDVVVAEGAGGAAEINLLDRDLVNLPLARAA
                     GMPAVLVVDIERGGAFAAAHGTIDLVPPDLARCIGGIVLNRFRGDPALLGDGLADLEN
                     RTGVPVLGVLPHLGPRALLGTEDSLDLGNVHDPDRRGSVDPVRVAAVQLPHLANPSDL
                     DPLVAEPDVVVRWVTSPGELADADLVVVPGSRATVDDLAWLRRTGLAAALVAAAERGT
                     DVLGICAGQQILGTRITDDVESGAGAVDGLGLLDVVTAFEQDKVVRRRTGFTIAGRAD
                     VTGYQIHLGRARGTDPWLALDPLETGGPVEHEGSRSGDGCVRGTSLHGLFDADGVRAA
                     ILSDVADRRGRTFAAHPTAFADRLEAQHDLLADWIDEHLDTGAVRDLAAVAAAPGSEP
                     GW"
     gene            6642..7319
                     /locus_tag="VLR27_14005"
     CDS             6642..7319
                     /locus_tag="VLR27_14005"
                     /inference="COORDINATES: protein motif:HMM:NF014060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HSP04620.1"
                     /translation="MSELGRHVARPAPVDVVDLPDGAGARAVCSWVFDAPRAALSSAP
                     VGGGSSAIEWLLNIGVPADYDRTDLDTHAREIAGRLGLQGTGAAMLTAVDVHRTVRAE
                     DGDVVVDATVGVTKPTWAADVDGSHGHRIDGRWEPGTINIVVQVPVALSPAAAVNAVI
                     TATEAKAQALQEAGVPGTGTASDAVTVVWPPAVPVEAFAGPRSTWGARIGRAVHAAIR
                     DGLERHP"
     gene            7316..7840
                     /locus_tag="VLR27_14010"
     CDS             7316..7840
                     /locus_tag="VLR27_14010"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF014351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HSP04621.1"
                     /translation="MIILVLGGTRSGKSVVAESMAARLAGPDGAVTYVATAQLDPTDA
                     DHAARIDAHRSRRPASWTTVECDRPADLPDLLRATDGVVLLDSLGTWVTGHPDLDPDA
                     GPLVDALRARRGTTIVVSEEVGLAPHAPTELGRRFADALGQANQAVSAVADHALLVVA
                     GRAIILPAPGDLPC"
     gene            7834..8532
                     /locus_tag="VLR27_14015"
     CDS             7834..8532
                     /locus_tag="VLR27_14015"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HSP04622.1"
                     /translation="MLSALGFLTVFGGARTPDRTTFLFFPVVGALIGAALAGVWLVSV
                     ELWSPGVAAVLVVAADLAITGMLHADGLADTADGLLPHLDRDRRLAVMAAPDVGAFAL
                     AVVPVVLLARWATLATDLVEPLSLIAVWAASRTVLAVVPALVPYARDAGLASAMVPGA
                     RRWTALWLVPAAGVLVVAQGATGAIALVALLATAAGVVLLARRRVGGFTGDVLGACAV
                     LAETIALLALAARP"
     gene            8529..9455
                     /locus_tag="VLR27_14020"
     CDS             8529..9455
                     /locus_tag="VLR27_14020"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HSP04623.1"
                     /translation="MRRPDVAARARGTALGLVLAQVVPPVPDRAHPVARFGALMTEVE
                     DLLWADDRRAGIRYTAAGAALGAVGGRLLGSTAAAVSVTTAGRQLREVAADIGAHLEA
                     GDLDAARTALPSLVGRDPSALDAVGIAAAVVESVAENMVDAVYAPALWGLAGGAAGTG
                     LYRALNTMDAMVGHRSERHERFGWASARADDLANLVPARLLALCVVALAPVERRAETV
                     RAIREDAPTHPSPNAGVAEAAFAGALGVSLGGPLRYGDRHEDRPTLGSGDRPGPADIA
                     RAVRLAEQAELVLIATMAGIGILAIRPRRRRR"
     gene            9452..10378
                     /locus_tag="VLR27_14025"
     CDS             9452..10378
                     /locus_tag="VLR27_14025"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSP04624.1"
                     /translation="MTGPLTPGPHGGDVERIAASLGRPADELLDLSASLNPLAPDALP
                     ALRAALDRVDRYPDDTRATAALAEAIGVDPTRLVLTNGGAEAIALVARHRPVGWADEC
                     DFSLYRRHLARLDPSGPRWRSDPHNPTGRLAAADDVADVRDEAFYLLATGGWTRGDDD
                     TTVLGSLTKAWAIPGIRVGYVLARDKDEADAIRTLRPRWSVNGLVCAALPALLDLADP
                     TGWRDGIAALRSQLHTLLVGHGYEPEPSDAPYVWVPRAAGLRDRLLPGGVVVRDGGTF
                     GHPDAVRIAVPGADGLERLDRALSATDHRSDP"
     gene            10375..>10925
                     /locus_tag="VLR27_14030"
     CDS             10375..>10925
                     /locus_tag="VLR27_14030"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF014347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HSP04625.1"
                     /translation="MNPDQLDHLRQRITTPAEDGERAEASRSATARWSTRAKPPGSLG
                     RLEDVAVRIAGITGTCPPAVPEQPAVVVFAGDHGVVADGASAWPSEITAAMVRTMAAG
                     GAAINAFAAQAGASVTLVDVGVSSDLSDLHALGRPDVLDRRVRAGTDSIAHGPAMTRA
                     DALAALAVGAAAADEAMDAGADLL"
BASE COUNT         1332 a         4287 c         3854 g         1452 t
ORIGIN      
        1 ggtggagcag ctgctcgacg atggagatgg cctggggcgg gccggggacg aggcggttcc
       61 acagcacgta ggccgccatc acgccggcca gccggtcgcc gagctcgtcc tggtgcagca
      121 cgatcttgcc gccgtcgacc aggagctggc gcagctcggg gaacagggtg gcgaggaagg
      181 tctcgcggtc gtcggtgcgg cggaaggggc ggtgccggta gggcatgccc atctcgtcgt
      241 agttgtgcag gttgtgggga gacgcggcga gcgacagcac catcgtgaag ccctgctcgc
      301 gcagccagat gatctcctcc tggcggcgca ccttgcggtg gttctgcccg tagccgccgg
      361 gacgctcgca gacggcgagc ttgccctgga ggacccaggt gaagttccgg ggctcgatgc
      421 cctgggccca cttgccgcgc agcaccatca ggtgccccct gggccaggcg cagcgagggc
      481 cgcgtcgagt gcggccaccg tggcgcggac gtcgtgcact cggatcatgg cggcacccat
      541 cgtggcagcc cagcaggcgg tcgtgatcga tccctccagc cggtcgtccg gaccgggccg
      601 gtcgtcggtg ccgtcggcct cggccaagac ggcgcccagg aaccccttgc ggctcgtccc
      661 cacggccacc gggatgccgg tggcgacgag ctggtccaga gccccgagca gggcccagtt
      721 gtgctcgaag gtcttcccga agccgatacc gggatcgatc cagatctcgt ccaccccggc
      781 gtccctgccc cgctgctcgg ccgccacgag gaacgcccgg acctcggcga ccacgtcgtc
      841 gtagtgcggt gagtcctgca tggtccccgg ctcgccgagc atgtgcatgg ccacccagcc
      901 ggcgtcggcg tcgcccgcca cctgctcgag cgacgcggac acgtcgttga ggatcgtggc
      961 gccggcggcc agcgcggcgc gggcgacctc ggcgtggcgg gtgtcgaccg acagccgggc
     1021 acccgcggcg gcgacgtcgc cgaccagcgc ctcgatggtg ggcacgaccc gggcgagctc
     1081 ggtgtcgatg ccgacgggct cagcgccggg ccgggtggac tccccgccga cgtcgacgat
     1141 gtcggcaccc tcggcgagca gctctcggcc ccgtctcacc gcggcgtcgg tgtccgccca
     1201 ggcgccaccg tcggagaagg agtccggggt gacgttcagg acgcccatga ccagcggtcg
     1261 catcggccgc tcaccctatc gaccgcccgg cggccccgaa cccggactgg accggccgcg
     1321 gcggacccgg gctatggtca tcggccggtg gagtgacgaa gtccggtgag atcccggcac
     1381 tgacccgcaa cggtgatgtc cgaggcctcc gggccgaggg cgagtccggt cgaaccctcc
     1441 cccgaacgaa ccatgtgacc ctcgaggcaa ggggttggtt cgaccggcac ccctgtcgat
     1501 cgagcagacc ggaacctcga cgacaggagg taccgaattg cgctccccca tccgaacccg
     1561 cctcgtggcg gtcctcgccg cgctcgccct ggcgctcgcc gcctgcggtg acgacgacga
     1621 cagcggcgtc gccgccagcg gcgaccccac cgacaccacc gaggctgccg acgacggcag
     1681 taccgaggag ggcgccgagg aggcgacgtt ccccgtcacg gtcaccgtcg acggcaccga
     1741 caccacgatc gaggccgaac cgcaggcgat cgtctcgctg tcgcccacgg cgaccgagat
     1801 gctgttcgcc gtcggcgccg gcgagcaggt catcgccgtc gacgacttct ccaacttccc
     1861 cgaggacgcc cccgtcaccg atctctccgg gttcgatccc aacgtggagg ccatcatcgg
     1921 ctacgacccc gacctcgtca tcctgtccgg tgaccgcaac gacgtcgtct ccggcctcgc
     1981 tgccgtcgat gtccccacga tcgtgctgcc gtcggccacg acgatcgagg acacctacac
     2041 ccagatcgag gtggtcggcg ccgcgacggg ccacgtcggc gacgcagccg aagtggtcgc
     2101 gaacatgcag tccgacatcg atgagctggt cgcccagctg cccgaacagg acgagccgct
     2161 gacctactac cacgagctgg acgacacgct ctacagcgtc acgtccgaca ccttcatcgg
     2221 cgagatctac gggctcgccg gcctggagaa cgttgccgac accgcggacg gggcagccga
     2281 ggccggcggc tacccgcagc tgtcggcgga gttcctgatc gacgccgatc ccgacctgat
     2341 cttcctcgcc gacaccaagt gctgcggcca ggacgccagc accgtggccg agcgtccggg
     2401 ctggagtgag atgagcgccg tccagcaggg cgacgtcgtc gagctggacg atgacatcgc
     2461 ctcccgctgg ggcccgagga tcgtggactt cctcgaggcg atcgtcgagg ccacggccga
     2521 cgtcgacgcc ggcgcggcgg gttgagcggc cggtcatgac gacggcgacc gacggtccgg
     2581 gcatcaccac cggtccgtcg gtcgcgcccg tcgaaccgcc agacggcgac gacctcgtcg
     2641 ggacgctgcc cgccgcgacc gacctgtccc cgcgatggct gctggcggga ttggctgccg
     2701 tggccgtcgc caccgtcgtc ggcatcgcca cgggtcccgt ctcgttgccc ctgcaccggg
     2761 tcgccgtcga gctggtcgac cacctgcccc tcgtgtcgat cgactccggg ctgtccgaca
     2821 cccagcgggc gatcgtgtgg gagatccgca cccctcgcgt cgtgctcggc ctgctcgtgg
     2881 gtgcgctgct cgccggctcg ggtgcgggct accagggcgt gttccgcaac ccgctcgccg
     2941 acccctacct gctcggcatc gccgccggcg cgggcctcgg cgccaccttg gcgatcgtca
     3001 ccggcgccgg agacggcgtc ggtgccttcg acgccatccc gatgttcgcc ttcatcggcg
     3061 ccctcgtggc cgtggcgatg gccgccgcgc tcagcgtgcg ccgcgacgga tccggcgggc
     3121 cggcgacact gatcctggcg ggcgtcgccg tggccgcgtt cttcaccgcg gcccagacct
     3181 acgtgcagca acgccacgcc gagaccctcc gccaggtcta cgcctggatc ctcggccggc
     3241 tctccaccag cggctgggac gaggtcctgc tgctcctccc ctacgcgctg atctgcggcg
     3301 ccgtgctcgt gctggcggtc ggcgccctcg acgtcctcgc tgtcggcgac gacgaggccc
     3361 gggccctcgg cctccacccg cgccgcatcc gcatggcggt cctcgtcgcc gcctcgctgg
     3421 cgaccgccgc cgcggtcgcg gtcagcgggc tgatcggctt cgtgggcctc gtggtccccc
     3481 acggcgtccg cctcgtcgcc ggcgtcagca accgccgcgt catcccgctc tcgttcctgt
     3541 tcgggggcgc cttcctggcg ctggccgacg tcctcgcccg cacggtgcag gcccccgccg
     3601 agctgccgat cggcgtcatc accgccttcg tgggcgcccc gttcttcttg tggatcctcc
     3661 ggtcgaggtc catggcatga gcacccaccc ggccatctcg tggcgcgacg tcggcgtcga
     3721 gctcggcggg tccaccgtcg tgcgcgacgt cagcctggac gtccccaccg gggcgtggct
     3781 gtcgctgatc ggcccgaacg gcgccggcaa gaccacgctg gtgcgcacgc tcggcgggac
     3841 ggtcgaccac gccggcgacg tcgagctgct gggccgcgac gggcggggcc tcagcgtgcg
     3901 cgaccgggcc cgccagctcg cagtcgtccc gcagcacccg gtgatcccac ccggcatgcg
     3961 ggtgttcgac tacgtcctcc tcgggcgggc gccccaccag ggcctccggt gcgcgtcgtc
     4021 gctccaggac cgtcgcaggt ccctcgcggt gcttcagcgc cttgggctcg aggacttcgc
     4081 cgaccggcgc gtcgacacgc tctcaggcgg tgagcgccag cgcgtggtgc tggctcgcgc
     4141 cctggtccag gacacccccg tgctcgtcct cgacgagccg accgcggcgc tcgacgtggg
     4201 ccaccagctc gaggtgctcg agctgatcgc cgacctgcgc accgagcgcg agctgaccat
     4261 cgtcacgacc gtccacgatc tcaccgtggc cggccagttc gccgacctcg tcgccgtgct
     4321 ggccgacggg cgcctcgtcg ccgcgggcac cccccgggag gtcctcacgc cggacgtgat
     4381 cggcgcccac tggggcgtgg aggccagctg cgacgtcgac gcagaggacg gctcggtgac
     4441 ggtgaccgtc cgccggcgca aggagcagcg ccgccgacca gcgacgcccg ccaccaccga
     4501 gctcgcaccg cacgccaccg aggaggacca ccgatgagcg acaccccgac cgacgcaccg
     4561 cccaccgagc atcaggagcc accggagctg cggcgcgccg actccctggt cctggtgaac
     4621 accggcaacg gcaagggcaa gtccaccgca gcgttcggca ccatgatccg ctccgtcgcg
     4681 cgcggctggc cggtggcggt cgtccagttc ctcaagtccg ggaagtggaa cacgggcgag
     4741 gagaaggtcg cccgtgacct cggcgtcgag tggttcgcca tcggcgaggg cttcacctgg
     4801 gacagcgagg acctgagccg cgacgaagcg gtggcccggg aggcctgggc tcgagcggcg
     4861 tcgctcctcg agtcgggcac ccaccgcctg gtcgtgctcg acgagatcac ctacccgatg
     4921 aactggggtt ggttcgacac cgacgaggtc gtcgccgcca tccgggatcg atcgccgaag
     4981 gtcaacgtca tctgcaccgg tcgcgacgca ccggagccgc tgctcgagct ggccgacacg
     5041 gccaccgaga tgcgggtcct caagcacgcg taccagtcgg gcatccgggc gatgaagggc
     5101 atcgactact agatgggcgc gccgccgcac ggtgcgctcc tcgtggtcgg cgccacgagc
     5161 ggcgcgggga agtcgaccgt gaccgccggc ctgtgccgct ggttcgcccg caccggcgcg
     5221 acggtggcgc cgttcaaggc ccagaacatg tcgaaccact cggcggtctc cgtggacggc
     5281 ggcgaagtcg gccgggctca ggcgatgcag gcggcggccg ccggcgtcga cgtcgagtcg
     5341 accatgaacc cgatcctgct gaagccctcc agcgcgacgg gcagccacgt cgtcgtgctc
     5401 ggcagggagg tggggaccac cgacgccgcc gggtggggtg tccgcaccga cgagctgcga
     5461 ccggtcgtgc tcgacgctct cacctcgctg cgccggcgct tcgacgtggt cgtcgccgag
     5521 ggtgccggcg gcgcggccga gatcaacctg ctcgaccggg acctggtcaa cctccccctc
     5581 gcccgtgccg ccgggatgcc ggcggtgctc gtggtcgaca tcgagcgtgg cggcgcgttc
     5641 gccgccgccc acgggacgat cgacctggtg ccgccggacc tcgcccgctg catcggcggc
     5701 atcgtcctga accgcttccg gggcgacccc gcgctcctcg gcgacggcct cgccgacctc
     5761 gagaaccgca ccggggtgcc ggtgctcggg gtgctgcccc acctcggccc ccgcgcgctg
     5821 ctcggcaccg aggactccct cgacctgggc aacgtccacg atcccgatcg ccgcggttcg
     5881 gtcgacccgg tgcgcgtggc cgccgtgcag ctcccgcacc tcgccaaccc gtccgacctc
     5941 gaccccctcg tcgccgagcc cgacgtcgtc gtccgctggg tgaccagccc gggtgagctg
     6001 gccgatgccg acctcgtcgt ggtgcccggc agccgggcga ccgtcgacga cctggcctgg
     6061 cttcgtcgaa cgggcctcgc ggctgcgttg gtcgcggcgg cggagcgcgg caccgacgtc
     6121 ctcggcatct gcgccgggca gcagatcctc ggcacgcgga tcacggacga cgtcgagtcc
     6181 ggggcgggcg cggtcgacgg gctcggcctg ctcgacgtgg tgaccgcgtt cgagcaggac
     6241 aaggtggtgc gccggcgcac cggcttcacc atcgccgggc gggccgacgt caccggctac
     6301 cagatccacc tggggcgggc gcgcggcacc gacccgtggt tggccctcga cccgctcgag
     6361 accggcgggc cggtggagca cgagggctcc cggtcgggcg acgggtgcgt acgggggacc
     6421 agcctgcacg gcctgttcga cgccgacggc gtccgcgctg ccatcctctc cgacgtggcc
     6481 gaccgacggg gcaggacctt tgcagcgcac ccgacggcct tcgccgatcg gctcgaggcc
     6541 cagcacgacc tgctggccga ctggatcgac gagcacctcg acaccggcgc ggtgcgcgac
     6601 ctcgccgccg tcgcagccgc gccgggctcg gagccgggct ggtgagcgag ctcggtcgcc
     6661 acgtcgcccg cccagcgccg gtcgatgtcg tggatctccc cgacggcgcc ggcgcgcgtg
     6721 ccgtctgcag ctgggtgttc gacgcccccc gggctgcgct ctcgtcggct ccggtcggtg
     6781 gcggcagctc ggcgatcgag tggctgctca acatcggggt gccggccgac tacgaccgca
     6841 ccgatctgga cacccacgcc cgcgagatcg ccggccggct cggcctgcag ggcaccggcg
     6901 cggcgatgct caccgccgtc gacgtgcacc gcaccgtgcg ggccgaggac ggcgacgtgg
     6961 tggtcgacgc caccgtcggc gtgaccaagc cgacctgggc tgccgacgtc gacggctccc
     7021 acgggcaccg catcgacggc cgctgggagc cgggcacgat caacatcgtc gtgcaggtcc
     7081 cggtggcgct gtccccggcg gccgccgtga acgcggtgat caccgccacc gaggccaagg
     7141 cccaggcgct gcaggaggcc ggggtgcccg gcaccggcac ggccagcgat gccgtgaccg
     7201 tcgtgtggcc gcccgccgtg ccggtcgagg ccttcgccgg gccccggtcc acctgggggg
     7261 cccgcatcgg ccgggccgtg cacgccgcga tccgcgacgg gctggagcgg cacccgtgat
     7321 catcctcgtc ctcgggggga cacgctcggg caagtcggtc gtcgccgagt cgatggccgc
     7381 gcgcctggcc ggtcccgacg gcgccgtgac ctacgtggcc actgcgcagc tcgaccccac
     7441 cgacgccgac cacgccgccc gcatcgatgc ccaccggagt cgccgcccgg cttcgtggac
     7501 gaccgtcgag tgcgatcgac ccgctgacct ccccgacctg ctgcgagcca ccgacggcgt
     7561 cgtgctcctc gactccctgg gcacgtgggt caccggccac ccggacctcg atcccgacgc
     7621 gggaccactg gtcgacgcac tccgggcgcg gcgcggcacg acgatcgtgg tgagcgagga
     7681 ggtggggctc gccccgcacg cccccaccga gctcggtcgc cgcttcgccg atgcgctcgg
     7741 ccaggccaac caggcggtgt ccgccgtggc cgaccacgcc ctgctcgtcg tggccggccg
     7801 ggcgatcatc ctgccggcac cgggcgacct gccgtgctga gcgccctcgg gttcctcacg
     7861 gtcttcggcg gagcacggac gccggaccgc acgacgttcc tcttcttccc ggtcgtcggc
     7921 gccctcatcg gcgccgcgct cgccggcgtc tggctcgtgt cggtcgagct gtggtcgccc
     7981 ggcgtcgccg ccgtgctcgt cgtggccgcc gacctcgcca tcaccgggat gctccacgcc
     8041 gacgggctgg ccgacaccgc cgacgggctc ctcccgcacc tcgaccggga ccggcgcctg
     8101 gccgtcatgg ccgctcccga cgtcggcgcg ttcgccctcg ccgtggtccc cgtggtgctg
     8161 ctggcgcggt gggccacgct cgccaccgat ctggtcgagc ccctctcgct catcgccgtg
     8221 tgggcggcga gccgcacggt gctcgccgtg gtgcccgccc tcgtgcccta cgcccgcgac
     8281 gccggcctcg cgtcggccat ggtgcccggc gcccgccggt ggaccgcgct gtggctggtg
     8341 ccggcggccg gcgtgctggt cgtggcgcag ggggccaccg gtgccatcgc cctcgtcgcg
     8401 ctgctcgcca ccgcggccgg ggtcgtcctg ctcgcccgac gccgcgtcgg cgggttcacc
     8461 ggcgacgtgc tcggggcgtg cgccgtgctc gccgagacca tcgccctgct cgcgctggcg
     8521 gcacgaccgt gaggcgaccg gacgtcgcgg cgcgggcccg gggcaccgcg ctcgggctcg
     8581 tcctggccca ggtcgtcccg ccggtacccg accgggccca cccggtcgcc cgcttcggcg
     8641 cgctgatgac cgaggtcgag gacctgctgt gggccgacga ccgtcgcgcc ggcatccgct
     8701 acaccgccgc gggcgcggcg ctcggcgccg tcggtggccg gctcctcggc tcgaccgccg
     8761 ccgccgtctc ggtgacgacc gctggtcgcc agctgcggga ggtcgccgcc gacatcggcg
     8821 cccacctcga ggccggcgac ctcgacgccg cccggaccgc actcccctcc ctcgtcggac
     8881 gcgacccgag cgcgctcgac gccgtaggca tcgcggccgc tgtcgtcgag tcggtggccg
     8941 agaacatggt ggatgccgtg tacgcgccgg ccctgtgggg cctcgccggc ggggcggcgg
     9001 gcaccggcct ctaccgggcg ctcaacacga tggacgccat ggtcggccac cgcagcgagc
     9061 gccacgagcg cttcgggtgg gcgtccgccc gggccgacga cctcgccaac ctcgtcccgg
     9121 cccggttgct cgccctctgc gtcgtggccc tggccccggt cgagcggcgc gccgagaccg
     9181 tgcgggccat ccgcgaggac gcccccaccc acccgtcacc gaacgccggc gtggccgaag
     9241 ccgcgttcgc cggtgccctg ggcgtgtccc tcggtggacc actccgctac ggcgatcgcc
     9301 acgaggaccg gcccacgctc ggctccggcg accgacccgg cccggccgac atcgcccgcg
     9361 ccgtccgcct ggccgagcag gccgagctgg tgctcatcgc caccatggcg ggcatcggga
     9421 tcctcgccat ccgcccccgg cggcgacgcc ggtgaccggc cccctgaccc ccggccccca
     9481 cggcggcgac gtcgagcgga tcgccgcttc cctcggccgg ccggccgacg agctgctcga
     9541 cctgtcggcc agcctcaacc cgctcgcgcc cgacgccctc cccgccctgc gcgctgcact
     9601 cgaccgggtc gaccgctacc ccgacgacac ccgcgccacc gccgcgctcg ccgaggccat
     9661 cggcgtcgac ccgacgcggc tggtgctgac caacggcggc gccgaggcca tcgccctcgt
     9721 cgcccggcac cgcccggtcg gctgggccga cgagtgcgac ttctcgttgt accggcgcca
     9781 cctggcgcgc ctcgatccgt ccggcccgcg ctggcggtcc gacccgcaca accccaccgg
     9841 gcgcctcgcc gcggccgacg acgtcgccga cgtgcgcgac gaggccttct acctgctggc
     9901 cacgggcggc tggacccggg gcgacgacga cacgaccgtg ctcgggtccc tgaccaaggc
     9961 gtgggcgatc ccgggcatcc gggttggcta cgtgctcgcc cgcgacaagg acgaggccga
    10021 cgccatccgc accctccggc ctcgctggtc ggtcaacggc ctggtgtgcg ccgccctgcc
    10081 tgcgctcctc gacctggccg atccgaccgg gtggcgagac ggcatcgctg cgctgcggtc
    10141 ccagctccac accctgctcg tcggccacgg ctacgaaccc gagccgagcg acgcccccta
    10201 cgtgtgggta ccccgagctg ccgggctgcg cgaccgcctc ctgcccggag gcgtcgtcgt
    10261 ccgcgacggc gggacgttcg ggcacccgga cgctgtccgc atcgccgtcc ccggagcgga
    10321 cggcctcgag cggctcgacc gcgccctgtc cgcaaccgac caccggagcg acccgtgaac
    10381 cccgaccagc tcgaccacct gcgccagcgg atcacgacgc ccgccgagga cggcgagcgc
    10441 gcggaggctt cacgatcggc gaccgcacgc tggagcaccc gggccaagcc ccccggctcc
    10501 ctcggccgcc tcgaggacgt cgccgtccgc atcgccggca tcaccgggac gtgcccgccg
    10561 gccgtgcccg agcagccggc cgtcgtcgtg ttcgccggcg accacggcgt ggtggccgac
    10621 ggggccagcg cctggccgag cgagatcacc gcggccatgg tgcggaccat ggcggccggc
    10681 ggggccgcca tcaacgcgtt cgccgcccag gccggcgcct cggtgacgct ggtcgacgtc
    10741 ggcgtgtctt cggacctcag cgatctgcac gccctcggcc gccccgacgt cctggaccgg
    10801 cgggtgcggg ccggcaccga cagcatcgcc cacggtcccg ccatgacccg ggccgatgcc
    10861 ctggccgccc tggcggtggg cgccgccgct gcggacgagg cgatggacgc cggcgccgac
    10921 ctgct
//
LOCUS       DATISU010000351         1632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_678342, whole genome shotgun sequence.
ACCESSION   DATISU010000351 DATISU010000000
VERSION     DATISU010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678342"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VLR27_14035"
     CDS             complement(<1..482)
                     /locus_tag="VLR27_14035"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP04626.1"
                     /translation="MGDDGVAYADLGDVTICHRLAGPEDGPRVVYVSGSGSDLRREPN
                     VFSSPFVERCRVLAYDHRGLGRSTTGSGPPTMADFAGDLLGLLDHVGWDTCRAVGISF
                     GGMVLMEAAATEPARFERCVLGCTSPGGEGGASFPLHELVGLPDDERRERWLDALDTR
                     "
     gene            complement(487..981)
                     /locus_tag="VLR27_14040"
     CDS             complement(487..981)
                     /locus_tag="VLR27_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04627.1"
                     /translation="MKRFVRFAFLAAALAAVASAVKSMRRPNDPLPTPASGPSEPWPP
                     LRADAAPTPDAAPAPNTPDPAAEAPAAEDLASPKPESTDEDTVRFPEPDGVPVAATPT
                     QGASWTEPTDDGGCPDGYPIKAKMSSKIFHCPGQLNYDRTTPDRCYVDETAAEADGLR
                     AAKR"
     gene            complement(978..>1632)
                     /locus_tag="VLR27_14045"
     CDS             complement(978..>1632)
                     /locus_tag="VLR27_14045"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HSP04628.1"
                     /translation="SIDLFEDRPAVSFRESLELALRRVEDVDVRTRWSDASLPGQTPA
                     DPLPSDPDWSGGTVFIDEQQASADAPADAVFAVAAGIGGDRGWFVADLLWTLRGIADK
                     LVGGPGMRRGRRHPDHLRVGDALDFWRVEAYEPPRLLRLRAEMRLPGEAWMEWVVEDQ
                     GPGRSLLRQRALFVPRGLFGRLYWYAVLPFHGLIFGKLAADIARRAGSVEGSVGSGR"
BASE COUNT          214 a          578 c          641 g          199 t
ORIGIN      
        1 ttgcgggtgt cgagggcgtc gagccagcgc tcgcggcgct cgtcgtcggg caggccgacc
       61 agctcgtgga gcgggaacga ggcaccgccc tctccgcccg gtgacgtgca gccgagcacg
      121 cagcgctcga accgagcagg ctccgtggcg gcggcctcca tcaggaccat gccgccgaag
      181 ctgatcccca cggcacggca cgtgtcccag ccgacgtggt cgaggaggcc caggaggtcg
      241 ccggcgaagt cggccatggt cggcggcccg gaacccgtgg tggagcggcc gaggccccgg
      301 tggtcgtagg cgagcacccg gcagcgctcg acgaacggcg acgagaagac gttgggctcc
      361 cgccgaaggt ccgacccgga gccgctgacg tacacgactc gcggcccgtc ctcggggcct
      421 gcgaggcggt ggcagatcgt gacgtcgccg aggtcggcgt aggcgacgcc gtcgtctccc
      481 atcaggtcag cgcttggcgg cgcggaggcc gtcggcctcg gctgcggtct cgtcgacgta
      541 gcagcggtcc ggcgtcgtgc ggtcgtagtt cagctggccg gggcagtgga agatcttcga
      601 gctcatcttc gccttgatgg ggtacccgtc ggggcacccg ccgtcgtccg tgggctcggt
      661 ccacgacgcg ccctgcgtcg gcgtggcggc gaccgggacg ccgtcgggct cggggaagcg
      721 gacggtgtcc tcgtcggtcg actccggctt cgggctggcg aggtcctcgg cggccggtgc
      781 ttcggcggcg gggtcgggcg tgttcggtgc cggcgcggcg tcgggggtgg gggcggcgtc
      841 ggcgcgcagc ggcggccacg gctcggacgg accgctcgcc ggcgtgggca gcgggtcgtt
      901 cgggcggcgc atcgacttca cggcggaggc gaccgcggcc agggcggcgg cgaggaaggc
      961 gaaccggacg aagcgcttca tcggccggac cctaccgacc cctccacgga tccggcccgc
     1021 cgagcgatgt cggcggcgag cttgccgaag atcaggccgt ggaacgggag cacggcgtac
     1081 cagtagaggc gaccgaacaa gccgcgcggc acgaacagcg cccgctggcg cagcagcgac
     1141 cggcccggtc cttggtcctc gaccacccac tccatccacg cctcgccggg cagtcgcatc
     1201 tcggcccgca gccgcagcag ccggggcggc tcgtaggcct cgacccgcca gaagtcgagg
     1261 gcgtcgccga cccgcaggtg gtcggggtgg cggcggcccc gtcgcatgcc cgggccgccg
     1321 acgagcttgt cggcgatgcc ccgcaacgtc cagaggaggt cggccacgaa ccagccccgg
     1381 tcgcccccga tgccggcagc gaccgcgaac accgcgtcgg ccggtgcgtc ggcggacgcc
     1441 tgctgctcgt cgatgaacac ggtgccgccc gaccagtccg ggtcggaggg gagcgggtcg
     1501 gcgggggtct ggccgggcag cgacgcgtcc gaccagcggg tgcgcacgtc gacgtcctcg
     1561 acccggcgca gggccagctc cagcgactcc cggaacgaca ccgccggccg gtcctcgaac
     1621 aggtcgatgc tg
//
LOCUS       DATISU010000352         1765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_72978, whole genome shotgun sequence.
ACCESSION   DATISU010000352 DATISU010000000
VERSION     DATISU010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72978"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..846)
                     /locus_tag="VLR27_14050"
     CDS             complement(235..846)
                     /locus_tag="VLR27_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP04629.1"
                     /translation="MGDPVLAPLPKGRHQLSRQEVQDAQRIRLAVGMAEEMAESGYVG
                     TPVAAVLKRARVSRQTFYELYDDKHACFLDALDLVGAVLLADLRAETAPGDPLDRART
                     AVDAYLDTIVRHRAFARLFLVEVHAAGEEAMARRAALQDEVVDGLADLLGAVEPDAGF
                     ACRAYVAAIASLVTLPVVTGDEGALDALRAPLLGLLEDLAERI"
     gene            complement(839..1402)
                     /locus_tag="VLR27_14055"
     CDS             complement(839..1402)
                     /locus_tag="VLR27_14055"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HSP04630.1"
                     /translation="MSTARIAPGSRDDVGLLNWAFARASGVVAGTEPPNLFLTLGRHR
                     RLFRGWLRFAGHLMPGGKISRRDTELVILRVGHRNDCEYELAQHRRLARRAGLSDAEI
                     ARAADGPDAAGWSERDRMLLVVTDELHDQRDLTDETWTRLRSHLDEVESIELVLLVAH
                     YEMLATAIGTLRIEPDRPAPRPWSRRG"
     gene            complement(1399..>1765)
                     /locus_tag="VLR27_14060"
     CDS             complement(1399..>1765)
                     /locus_tag="VLR27_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HSP04631.1"
                     /translation="ALGNTLRAEIAPTGARVGVAYFAELDTEMTSRGFGTEAAKRFLG
                     GRTITRVTPLSVGLDALERGIARRSRFICAPAWAGAVLPIRMLAQPVMDLAFRGKVAG
                     ALEVARAESVELTTPQPVS"
BASE COUNT          241 a          634 c          674 g          216 t
ORIGIN      
        1 cggcgggttc ctcggcgccc tgctgttcgg cagcgacggc gacgagacca cgctcggccc
       61 ggccgggatc atcggctcga tcatcggtgc gatcatcgtg ctgctgatct acaacgccgt
      121 caccggccgc aaggacgcca ccgctcggcg ctgatccgcc ggcgcccggc gccgacatct
      181 gagccgacgc accgcaccgc cgctgctccc agcggcggtg tggcgcgtcc gggctcagat
      241 gcgctcggcc aggtcctcga gcagccccag gagcggcgca cggagcgcgt cgagcgcacc
      301 ctcgtcgccc gtgaccaccg gcagggtgac gagcgaggcg atcgccgcga cgtaggcccg
      361 gcacgcgaac ccggcgtcgg gctcgacggc cccgaggagg tcggccagcc cgtcgaccac
      421 ctcgtcctgc agcgcggccc ggcgcgccat ggcctcctcc ccggcagcgt gcacctccac
      481 caggaacagc cgagcgaatg cccggtggcg gacgatcgtg tcgaggtagg cgtcgaccgc
      541 ggtgcgcgcc cggtcgagcg ggtcgcccgg agccgtctcg gcgcgcagat cggcgaggag
      601 gaccgcaccc acgaggtcga gggcgtcgag gaagcaggcg tgcttgtcgt cgtagagctc
      661 gtagaaggtc tggcgggaca cccgggcacg cttgagcacg gcggcgacgg gggtgcccac
      721 gtaaccggac tcggccatct cctcggccat gcccaccgcg agccggatcc gttgcgcgtc
      781 ctggacctcc tggcggctca gctggtggcg ccccttcggc agcggggcca gcaccgggtc
      841 acccacggcg cgaccagggg cggggcgcgg gacggtcggg ctcgatccgc agcgtgccga
      901 tggcggtggc cagcatctcg tagtgggcga cgagcagcac gagctcgatc gattcgacct
      961 cgtcgaggtg gctgcggagc cgggtccacg tctcgtcggt gaggtcgcgc tggtcgtgga
     1021 gctcgtcggt cacgaccagg agcatccgat cgcgctcgga ccagccggcc gcgtcgggcc
     1081 cgtcggcggc gcgagcgatc tcggcatcgg acaggccggc ccggcgggcc agtcgccggt
     1141 gctgggccag ctcgtactcg cagtcgttgc ggtggccgac ccgcaggatc accagctcgg
     1201 tgtcgcggcg ggagatcttc ccgccgggca tgaggtggcc ggcgaagcgc agccagcctc
     1261 ggaacaaccg gcggtgccgg cccagcgtca ggaacaggtt gggcggctcg gtcccggcca
     1321 cgacccccga cgcccgggcg aaggcccagt tgaggaggcc gacgtcgtcc cggctgcccg
     1381 gggcgatgcg cgcggtgctc acgagacggg ctggggggtg gtgagctcca ccgactcagc
     1441 ccgggcgacc tcgagcgcac cggcgacctt gccgcggaac gcgaggtcca tgaccggctg
     1501 ggcgagcatc cggatgggca ggacggcgcc ggcccacgcc ggcgcgcaga tgaaccggct
     1561 ccggcgggcg atgccacgct cgagtgcatc gagaccgacc gacagcggcg tgacccgggt
     1621 gatcgtgcga ccgccgagga accgcttcgc cgcctcggtg ccgaagcccc ggctggtcat
     1681 ctcggtgtcg agctcggcga agtaggcgac cccgacccgg gcgcccgtgg gggcgatctc
     1741 ggcgcgcagg gtgttgccga gggcc
//
LOCUS       DATISU010000353         5585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_585460, whole genome shotgun sequence.
ACCESSION   DATISU010000353 DATISU010000000
VERSION     DATISU010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585460"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VLR27_14065"
     CDS             <1..703
                     /locus_tag="VLR27_14065"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSP04632.1"
                     /translation="GLIRVLYLGFQTYRVDWQPVIYAVAILTLLVGALMAVVQTDVKR
                     MLAYSSINHAGFVLVAVQAATERGVQASMFYLASYTFLVGGSFAIVTVVGRTGDDRHT
                     LDDYRGLAKARPALALVFALLLFSQAGVPFTSGFFAKFYAIAAAVDAGSYPLAAVAML
                     TAVIAAFLYLRITVSMFLGDVDDADVPDRRTLPVPVGAVIALVICVVVTLGVGIFPHV
                     LTGPAGDATAVLVGF"
     gene            717..1058
                     /locus_tag="VLR27_14070"
     CDS             717..1058
                     /locus_tag="VLR27_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04633.1"
                     /translation="MATIESAGDVVEVDDVLVTISPRPGRLARRRSTLTEPVTWPAPR
                     TTVALRRTDITGQWQARFTYEGQTVTLVFPPKRLDELVAFADAHLGGMTNTVMDAEQD
                     RLARRAEDGLR"
     gene            1055..1810
                     /locus_tag="VLR27_14075"
     CDS             1055..1810
                     /locus_tag="VLR27_14075"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSP04634.1"
                     /translation="MTIAESVLIAVAGFLAGGVNAVAGGGSLISFPALVAAGLPTLDA
                     NVTNTTAVWPGYLGSAAAYRSELRTQGARLRGLAVTSVLGAGAGAAILLVAPDAVFDA
                     VVPFLVLAGSVLLALQPRIAKAVLARTEAGGQGQTAQLHVAIFLAAVYGAYFGGGLGV
                     ILLAVLGIFVADHLQRLNGLKAAVSLLVNTVALVAFALFGPVDWGAVAIVAPASLLGG
                     YVGGRLARRLSPDALRATIVVFGVVVAVWLALR"
     gene            1887..2735
                     /locus_tag="VLR27_14080"
     CDS             1887..2735
                     /locus_tag="VLR27_14080"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HSP04635.1"
                     /translation="MAKSRAPHRRPGPRAVLLLAIGLALGFSAYHAIRALDAEDTGVA
                     IADDAAVTTSVPVTTTSTTQATTTTAAPTTTAAPTTTAAPTTTSTTTTTVPGPPTYRT
                     GDQGPEVAALQERLLALGFWVPVVDGDYGSVTQQAVMAFQKHAGLSRDGLAGPATLAA
                     LDTAPPVAAREGGDHVELDLDRQLLIVVRGGRTIAFNTSTGTTGWRTPPGRFTVQREI
                     DGVREAPLGDLYRPKYFNAGIAVHGSPSIPGYPASHGCARVHDRVMDLLWSDGLMPIG
                     TPVWVY"
     gene            2848..3213
                     /gene="ndhC"
                     /locus_tag="VLR27_14085"
     CDS             2848..3213
                     /gene="ndhC"
                     /locus_tag="VLR27_14085"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSP04636.1"
                     /translation="MSEFLRNYLTVAIFGGVAVALLAGVLGLGSLIRPTRRQEGKYEN
                     YESGVDPVGDTWSQSQIRYYIFALMFVIFDVEAVFIFPWATRVEEYGVYGLVMMLIFV
                     FILTLGLVHAWRKGVLRWI"
     gene            3227..3727
                     /locus_tag="VLR27_14090"
     CDS             3227..3727
                     /locus_tag="VLR27_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HSP04637.1"
                     /translation="MGLVESGKLPKPLTQLLNTSRKYSLWMYQWGLACCAIEMAAAMA
                     GPRFDIMRLGVIPLPASPRQSDLLCVSGTVTDKMVPAVRRLWEQMPDPKYVISMGACA
                     NCGGPYWDSYSVTKGVDQVIPVDVYVPGCPPRPEALLEGVVLLQERIQNEDMADKWRG
                     DPIVVS"
     gene            3714..4427
                     /locus_tag="VLR27_14095"
     CDS             3714..4427
                     /locus_tag="VLR27_14095"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSP04638.1"
                     /translation="MSSAEPEAPEQEATETDERREALLAGLADRLGDDLLGSHILPGR
                     DLWVRVTPAVWVSTMRHLRDDLGFRFFEFVSAIDWLPSPYGRSEESGLDVDLVARRAE
                     ALATDPSTLETGLAGGDTRYQVFARVMNLAERDLGIIVKVDVPPLADDPHLPGAVGTI
                     VPIYPGADWGEREVHEMFGIGFEGNPNLRNLYLPTGFEGYPLRKDYPLLARHVKPWPG
                     IVDVEPMPDEGGDEEEGDG"
     gene            4424..>5585
                     /locus_tag="VLR27_14100"
     CDS             4424..>5585
                     /locus_tag="VLR27_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D 1"
                     /protein_id="HSP04639.1"
                     /translation="MTVTESEQLAYVAAKAADARVNVELETDDGMTLNIGPQHPATHG
                     TLRIVAKLDGEQVLWVEPIMGYMHRGYEKLVEVRTYPQVTTLVNRIDWLAAACNEVPF
                     ILATEQLMGVEAPLRAQYLRTIFHEMGRIAHLTMFLGDMGVQIGALTPMFYAMRDREF
                     VLNQTESVSGGRFHPNFNRIGGLKDDIPKGWLDETIRVMKTMQAFCDQMEELLLGNEI
                     FEQRTRGIGIIPAEIAQQYGLSGANLRASGVDWDLRRDVDSPMAWKHADFNVVTHTDG
                     DCYARTWIRLQETREACKIVTQLCEDLPAGPIMAKVPRIIKVPAGEAYVATENPLGEM
                     GYYIVSQGDLGPFRVKIRTASFHNISIVPWVLRGVYVPDIISILGSLYFILGD"
BASE COUNT          770 a         2166 c         1787 g          862 t
ORIGIN      
        1 cggcctcatc cgggtgctct acctcggctt ccagacctac cgggtcgact ggcagccggt
       61 gatctacgcc gtggccatcc tcaccctgct cgtgggtgcc ctgatggccg tcgtccagac
      121 cgacgtcaag cggatgctgg cctactcctc gatcaaccac gccggcttcg tcctcgtcgc
      181 cgtgcaggcc gccaccgagc gcggcgtgca ggcctcgatg ttctacctgg ccagctacac
      241 cttcctggtc ggcggcagct tcgccatcgt caccgtcgtc gggcgcaccg gcgacgaccg
      301 ccacaccctc gacgactacc gaggcctggc caaggcccgc cccgcgctgg cgctggtctt
      361 cgccctgctg ctgttcagcc aggccggcgt tcccttcacc agcggcttct tcgccaagtt
      421 ctacgccatc gccgccgcgg tggacgccgg ctcgtacccg ctggccgcgg tcgcgatgct
      481 caccgcggtc atcgcggcct tcctgtacct gcgcatcacg gtgtcgatgt tcctcggcga
      541 cgtcgacgac gccgacgtcc ccgatcgccg cacgctgccg gtgcccgtcg gcgccgtcat
      601 cgcactggtc atctgcgtgg tcgtcaccct cggggtgggc atcttccccc acgtgctcac
      661 cggccccgcc ggcgacgcca ccgcggtcct cgtcgggttc tgacccgact ccagccgtgg
      721 caaccatcga gtcggccggc gacgtcgtcg aggtcgacga cgtgctggtc accatctcgc
      781 cccgcccggg ccgcctcgcc cgccggcgct ccacgctcac cgagccggtg acgtggccgg
      841 cgccgcgcac caccgtcgcg ctgcgccgca ccgacatcac cgggcagtgg caggcccggt
      901 tcacctacga gggccagacc gtcacgctgg tgttcccgcc caagcgcctc gacgagctcg
      961 tcgcgttcgc cgatgcccac ctcggcggca tgaccaacac cgtcatggat gccgagcagg
     1021 accgcctggc ccgccgcgcc gaggacgggt tgcggtgacc atcgccgagt ccgtgctgat
     1081 cgccgtcgcc gggttcctgg ccggcggcgt gaacgccgtc gcgggcggcg gatcgctcat
     1141 ctccttcccg gccctcgtcg ctgccgggct gccgaccctc gacgccaacg tcaccaacac
     1201 caccgcggtc tggcccggct acctgggcag cgccgccgcc taccgcagcg agctgcgcac
     1261 ccagggcgca cgcctgcgcg gcctggcggt gacctcggtg ctcggcgccg gcgcgggcgc
     1321 agcgatcctg ctcgtcgccc ccgacgcggt cttcgacgcg gtggtgccgt tcctcgtgct
     1381 cgccggatcg gtgctgctcg ccctgcagcc gcgcatcgcc aaggcggtcc tggcccgcac
     1441 ggaagccggc gggcagggcc agaccgcgca gctccacgtc gccatcttcc tggccgccgt
     1501 gtacggcgcg tacttcggtg gtggcctcgg ggtgatcctg ctcgcggtcc tcggcatctt
     1561 cgtcgccgac cacctgcagc ggctcaacgg gctcaaggcc gccgtctccc tgctggtcaa
     1621 caccgtcgcc ctcgtcgcgt tcgccctctt cggcccggtc gactggggcg cggtggcgat
     1681 cgtggcgccg gcgagcctgc tcggcggcta cgtcggcggc cggctggccc ggcgcctgtc
     1741 gcccgacgcg ctgcgggcga ccatcgtggt gttcggcgtc gtggtcgcgg tctggctcgc
     1801 gctgcgctga acgcgttcgc ctcccgggtg accgatgtca tagattttcc gtgaacggcg
     1861 gacggggctg gagcgtctca tcgacgatgg cgaaatcacg tgccccccac cgccgccccg
     1921 gcccccgcgc cgtgctgctc ctcgccatcg gcctggcgct cgggttcagc gcctaccacg
     1981 ccatccgggc gctcgacgcc gaggacaccg gcgtggcgat cgccgacgac gcggccgtga
     2041 ccacctcggt cccggtcacc accacctcga cgacgcaggc gacgaccacc accgccgcgc
     2101 ccaccacgac ggccgccccc acgaccaccg ccgcccccac caccacgtcc acgacgacca
     2161 ccaccgtccc cggcccgccc acgtaccgca ccggtgacca ggggcccgag gtcgccgccc
     2221 tgcaggagcg gctcctcgcc ctcgggttct gggtccccgt cgtcgacggg gactacggct
     2281 cggtgaccca gcaggccgtg atggcattcc agaagcacgc cggactgtcc cgggacggcc
     2341 tcgccggccc ggcgaccctg gccgccctcg acaccgcgcc cccggtggcg gcccgcgagg
     2401 gcggcgacca cgtcgagctc gacctcgatc gccagctgct catcgtcgtg cggggcgggc
     2461 gcaccatcgc gttcaacacc tccaccggga cgacgggctg gcggaccccg cccggacggt
     2521 tcaccgtcca gcgggagatc gacggcgtcc gggaggcgcc cctcggcgac ctctaccggc
     2581 ccaagtactt caacgccggc atcgccgtgc acggctcgcc gagcatcccc ggctaccccg
     2641 cctcccacgg ctgcgcacgg gtccacgacc gggtcatgga cctgctgtgg tcggacggcc
     2701 tgatgcccat cgggacgccc gtctgggtgt actgaacccg ggtccggagg ggccgagcgg
     2761 tcccgttttt cgggccccga ccggccgtct gcgaccggct tgggcgatct tggcccactt
     2821 ctccaccggt cagtagggtg cccctccgtg tcggagttcc tccgtaatta cctgacggtt
     2881 gccatcttcg gtggcgttgc ggtcgcgctg ttggccggtg tcctcggcct cggctcgctg
     2941 atccgtccga cccgtcgcca ggagggcaag tacgagaact acgagtccgg cgtcgacccg
     3001 gtcggagaca cctggtccca gagccagatc cggtactaca tcttcgccct gatgttcgtc
     3061 atcttcgacg tggaggcggt cttcatcttc ccgtgggcca cccgcgtcga ggagtacggc
     3121 gtctacggcc tcgtcatgat gctgatcttc gtcttcatcc tcacccttgg cctcgtgcac
     3181 gcgtggcgca agggtgtcct gcgctggatc taggagaggg aaccccatgg gtctggtcga
     3241 gagcggcaag ctccccaagc cgctcacgca gctgctcaac acgtcacgca agtactccct
     3301 ctggatgtac cagtggggcc tcgcgtgctg cgccatcgag atggccgcag ccatggccgg
     3361 gccgcgcttc gacatcatgc gcctcggcgt gatcccgctg ccggcgtcgc cgcgccagtc
     3421 cgacctgctc tgcgtgtccg gcacggtcac cgacaagatg gtgccggccg tgcgccgcct
     3481 gtgggagcag atgcccgacc cgaagtacgt gatctccatg ggcgcctgcg ccaactgcgg
     3541 cgggccctac tgggacagct actcggtgac caagggcgtc gaccaggtca tcccggtcga
     3601 cgtctacgtc ccggggtgcc cgccccgccc cgaagccctg ctcgagggcg tggtgctgct
     3661 ccaggagcgc atccagaacg aggacatggc cgacaagtgg cgaggtgatc ccattgtcgt
     3721 cagctgagcc cgaagccccc gagcaggagg ccaccgagac cgacgagcgc cgcgaggcgc
     3781 tcctggccgg cctggccgac cgactcggcg acgacctcct cggctcccac atcctgcccg
     3841 gccgcgacct gtgggtccgc gtgaccccgg ccgtctgggt gtccaccatg cgccacctgc
     3901 gcgacgacct cggcttccgc ttcttcgagt tcgtctccgc catcgactgg ctgccatcgc
     3961 cctacgggcg ctccgaggag tccggcctcg acgtcgatct ggtggcccgg cgggccgagg
     4021 cgctggccac cgaccccagc acgctcgaga ccgggctggc cggtggcgac acccgctacc
     4081 aggtgttcgc ccgggtcatg aacctggccg agcgcgacct cggcatcatc gtcaaggtcg
     4141 acgtgccccc gctcgccgac gatccccacc tgcccggggc cgtcggcacc atcgtgccca
     4201 tctaccccgg cgccgactgg ggcgagcgcg aggtccacga gatgttcggc atcggcttcg
     4261 agggcaaccc caacctgcgc aacctctacc tgcccaccgg cttcgagggg tacccgctgc
     4321 gcaaggacta cccgctgctg gcccggcacg tgaagccgtg gccgggcatc gtcgacgtcg
     4381 agcccatgcc cgacgaaggc ggcgacgagg aggagggcga cggatgaccg tcaccgagtc
     4441 agagcagctg gcctacgtcg ccgcaaaggc ggccgacgcc cgcgtcaacg tcgagctcga
     4501 gaccgacgac ggcatgacgc tgaacatcgg cccccagcac ccggccacgc acggcacgct
     4561 gcgcatcgtc gccaagctcg acggcgagca ggtcctctgg gtcgagccga tcatgggcta
     4621 catgcaccgg ggctacgaga agctcgtcga ggtccgcacg tacccgcagg tcaccacgct
     4681 ggtgaaccgc atcgactggc tcgccgccgc gtgcaacgag gtgccgttca tcctcgccac
     4741 cgagcagctc atgggcgtgg aggcgccgct gcgggcccag tacctgcgca cgatcttcca
     4801 cgagatgggc cgcatcgccc acctcaccat gttcctcggg gacatgggcg tgcagatcgg
     4861 cgcgctgacg ccgatgttct acgcgatgcg cgaccgcgag ttcgtgctga accagaccga
     4921 atcggtcagc ggtggccgct tccaccccaa cttcaaccgc atcggtggcc tcaaggacga
     4981 catcccgaag ggctggctcg acgagaccat ccgggtcatg aagacgatgc aggccttctg
     5041 cgaccagatg gaggagctgc tcctcggcaa cgagatcttc gagcagcgca cccgcggcat
     5101 cggcatcatc cccgccgaga tcgcccagca gtacggcctc tccggcgcca acctgcgggc
     5161 ctcgggcgtc gactgggacc tgcgccgcga cgtcgactcg cccatggcct ggaagcacgc
     5221 cgacttcaac gtggtcaccc acaccgacgg cgactgctac gcccgcacct ggatccgcct
     5281 gcaggagacc cgcgaggcct gcaagatcgt cacccagctg tgcgaggacc tgccggccgg
     5341 cccgatcatg gccaaggtgc cccgcatcat caaggtgccc gccggcgagg cctacgtcgc
     5401 caccgagaac cccctcggcg agatgggcta ctacatcgtc tcccagggcg acctcggccc
     5461 gttccgggtc aagatccgca ccgccagctt ccacaacatc tcgatcgtgc cgtgggtgct
     5521 ccgcggcgtc tacgtgccgg acatcatctc gatcctcggc tcgctgtact tcatcctggg
     5581 agaca
//
LOCUS       DATISU010000354         1537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_539002, whole genome shotgun sequence.
ACCESSION   DATISU010000354 DATISU010000000
VERSION     DATISU010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539002"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VLR27_14105"
     CDS             <1..462
                     /locus_tag="VLR27_14105"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04640.1"
                     /translation="VDIDRQWDVVLANRAALALVEGVPDHLLGPPLNVFRVCLHPDGL
                     AARTRNLSEWAGYLLHQIDRTVRLTGDRRLAEIADEVRAYPNVAGLPDQTPEEDPPLL
                     VPVVMDMDGTAVAMFTTLTTFGTPRDVTLDELAVELFFPADDAADAHLRAR"
     gene            complement(512..1198)
                     /locus_tag="VLR27_14110"
     CDS             complement(512..1198)
                     /locus_tag="VLR27_14110"
                     /inference="COORDINATES: protein motif:HMM:NF022794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
                     /protein_id="HSP04641.1"
                     /translation="MGLAAAVLVAAGCGGDGDVVERAETTTTSGPTSTVAVTTTTTEP
                     DGPVVIEVRTERRAMDGTDGFDQVVRDTLGDPRGWGQAGFEIRFSDDAPNVVLVAEGH
                     EVDALCDPYDTGGRYSCQIGPLVALNADRWREATDTWPGTLDEYRQMLVNHEVGHLLG
                     RHHARPACQEPGAPAAVMYQQSAGVEGCAPNPWPLPWEIECAARHDEPVAPPYEPDAS
                     ATCGPNDISG"
     gene            complement(1231..>1537)
                     /locus_tag="VLR27_14115"
     CDS             complement(1231..>1537)
                     /locus_tag="VLR27_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04642.1"
                     /translation="REDSDVYEGGAIAHPGFLARQANYVLSSSVRLGPWIHVSTRAHH
                     RGMVRDGELVEVRGVVTDEREHKGHRFVDLDVEITADGRPVWSAAHTAIWRPRQDRA"
BASE COUNT          193 a          595 c          531 g          218 t
ORIGIN      
        1 gtggacatcg accgtcagtg ggacgtggtg ctggccaacc gcgcggccct ggcgctcgtc
       61 gaaggcgtcc ccgaccacct gctcggcccg ccgctgaacg tgttccgggt ctgcttgcac
      121 ccggacggac tggccgcccg cacccgcaac ctgtccgagt gggccggcta cctgctgcac
      181 cagatcgacc ggaccgtgcg cctcaccggc gaccgacgcc tcgccgagat cgccgacgag
      241 gtgcgggcct acccgaacgt cgccgggctc cccgaccaga cgccggagga ggacccaccg
      301 ctgctggtgc cggtggtgat ggacatggac ggcaccgccg tcgccatgtt caccacgctc
      361 accaccttcg ggacgccacg cgacgtcacc ctcgacgagc tggccgtgga gctgttcttc
      421 cccgccgacg acgccgccga cgcccacctc cgcgcccgct gatccgtggc cacgcccttc
      481 ccgttctggc agttcccgtg tagcggcagc ctcagccgct gatgtcgttc gggccgcacg
      541 tggcggaggc gtcgggctcg tagggcgggg cgaccggctc gtcgtggcgg gcggcgcact
      601 cgatctccca gggcaggggc cacgggttcg gcgcgcaccc ctcgaccccc gcggactgct
      661 ggtacatcac cgctgccggt gcaccgggct cctggcaagc ggggcgggcg tggtggcggc
      721 cgaggaggtg gccgacctcg tggttcacca gcatctggcg gtactcgtcg agcgtccccg
      781 gccaggtgtc ggtcgcctcc cgccagcgat cggcgttcag cgccaccagc gggccgatct
      841 ggcacgagta ccgaccgccg gtgtcgtagg ggtcgcagag ggcatcgacc tcgtggcctt
      901 cggcgaccag caccacgttc ggggcgtcgt cgctgaaccg gatctcgaag ccggcctggc
      961 cccagcccct cgggtcgccg agggtgtcgc ggacgacctg gtcgaagccg tcggtcccgt
     1021 ccatggcccg ccgctcggtg cgcacctcga tcaccaccgg gccgtcgggc tccgtcgtcg
     1081 tcgtcgtcac cgccaccgtg gacgtcggac cggacgtggt cgtggtctcc gcgcgctcga
     1141 cgacgtcacc gtcaccgccg cacccggctg cgaccagcac cgcagccgcc agccccacca
     1201 cacgaacggc cccggcgccc gccaccaccc tcaggcccgg tcctggcggg ggcgccagat
     1261 cgccgtgtgc gcggcgctcc acacggggcg gccgtcggcc gtgatctcga cgtcgagatc
     1321 cacgaagcgg tggcccttgt gctcccgctc gtcagtgacc acgccgcgca cctcgaccag
     1381 ctcgccgtcg cgaaccatgc cccggtggtg ggcgcgggtg ctgacgtgga tccaggggcc
     1441 gaggcgcacg ctgctcgaca gcacgtagtt ggcctgacgg gcgaggaagc cgggatgggc
     1501 gatcgctccc ccctcgtaga cgtcgctgtc ctcccgg
//
LOCUS       DATISU010000355         3709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_73666, whole genome shotgun sequence.
ACCESSION   DATISU010000355 DATISU010000000
VERSION     DATISU010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73666"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..642)
                     /locus_tag="VLR27_14120"
     CDS             complement(43..642)
                     /locus_tag="VLR27_14120"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP04643.1"
                     /translation="MGYHHLAFASRDMQATHHFYADVLGFELVKAVAAPTDRPGGWAK
                     HLFYAIGEHDGSENAGALIAFWELHDERMADKDTAISTGLGFEAWVNHVAFQADGLGD
                     IDRRRTQWLDAGIDVMEIDHGFCTSIYTMDPNGILVEFCTDTAPYSQDDKDRALEILR
                     SDDPELEAPPTPVFHSAADRAALAEVAEPVTAAAGTPTT"
     gene            734..940
                     /locus_tag="VLR27_14125"
     CDS             734..940
                     /locus_tag="VLR27_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04644.1"
                     /translation="MFLGEDLLAYLVLAIGGAMAVGSILAIVRPPEQVKDGDLPAAPI
                     ARSIVFAVVGLIGAIWALASLVSG"
     gene            complement(950..2320)
                     /gene="murA"
                     /locus_tag="VLR27_14130"
     CDS             complement(950..2320)
                     /gene="murA"
                     /locus_tag="VLR27_14130"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HSP04645.1"
                     /translation="MTPAEPDAPAEAWSVEPSGPLRGEVRVAGSKNAITKHMVAAVMG
                     SSPSTIHNVPDVGDVGITTDVLQSLGVGVDLDGDTITVTPGDEIDPEVPLRYSGLNRI
                     PILLLGPLLHLHQEAFVPVVGGDRIGARPVDFHVDALRAFGAEVTETEDGVRAKVAGR
                     LVGTQITLPYPSVGATETVLLTAALAEGRTVLNNAATEPEIIELALFLQRMGARVELT
                     PGRRFVVDGVEKLHGATTRLQGDRLEAFSYLVAGLVTGGQVRVAGCAQERMVTAISTL
                     QRMGAELEINDDWISANAPNGLRAIAIHTDTHPGFMTDWQSPLFVLMTQAEGMSVLHE
                     TVFEDRIRYPAMVVQAMGGEIELFETCLGGPACRFHDGNAVHSAVVRGVSELHGAHVE
                     IPDLRAGFSGVIAAAVATGSSVIEGVHHLERGYNKPLETLQSLGLRVTRTEPNPATAT
                     SPEPEA"
     gene            complement(2394..3296)
                     /gene="pyrF"
                     /locus_tag="VLR27_14135"
     CDS             complement(2394..3296)
                     /gene="pyrF"
                     /locus_tag="VLR27_14135"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HSP04646.1"
                     /translation="MHPADALDARITATGSIACVGLDPRPDLLPGELIARHRTTGGAM
                     DAVASAFIEANQRVIDAVADRCAAVKPQAACYEAYGLAGWAALEATVRHATEAGIPVI
                     LDAKRGDIGSTAAHYGQMAFGGAPDLDGGTCAGIGADWLTVNGYLGSDGVEPVLAAGG
                     GVFVLVKTSNPASGELQDVELAGGGTVADRMADRVATWGADRLGACGLSDVGAVVGAT
                     YPDHAIALRERMPDTVFLVPGYGAQGASASDAVAGRRPDGRGVLVSSSRGITGAWREA
                     GTDDIGAAARTALDAMNADLDAAR"
     gene            3360..>3709
                     /locus_tag="VLR27_14140"
     CDS             3360..>3709
                     /locus_tag="VLR27_14140"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HSP04647.1"
                     /translation="MSRDVLKAASVPADDSREFAQRMTAGDPVTPSNEKPTTSRTRRR
                     RRFGLRRHGDGDERGAALVEFALIATPLFLILFGTIEFGWAFFQLNDIRHGAREGIRL
                     VAVDSDVTPTYTGS"
BASE COUNT          500 a         1277 c         1394 g          538 t
ORIGIN      
        1 cgatcagcgt gcgcggatcc tccgccggcg ggtcccccac gatcaggtgg tcggggtgcc
       61 cgctgcggcg gtgaccggct cggccacctc ggcgagggcg gcgcggtcgg ctgccgagtg
      121 gaacaccggc gtcggaggcg cctccagctc ggggtcgtcg ctgcgcagga tctccagcgc
      181 ccggtccttg tcgtcctggg agtagggggc ggtgtcggtg cagaactcca cgaggatccc
      241 gttcgggtcc atcgtgtaga tgctggtgca gaacccgtgg tcgatctcca tgacgtcgat
      301 gccggcatcg agccactggg tgcgccggcg gtcgatgtcg ccgaggccgt cggcctggaa
      361 ggcgacgtgg ttgacccacg cctcgaagcc gagaccggtg gagatggcgg tgtccttgtc
      421 ggccatccgc tcgtcgtgca gctcccagaa ggcgatgagc gcgccggcgt tctcgctgcc
      481 gtcgtgctcg ccgatggcgt agaagaggtg cttggcccaa ccgccgggcc ggtcggtcgg
      541 ggcggcgacc gccttgacca gctcgaagcc gaggacgtcg gcgtagaagt ggtgggtggc
      601 ctgcatgtcg cgcgaggcga aggccaggtg gtggtagccc atctcggatc ccctccggag
      661 tggaaacgtg cggagccagt gttccacaac cgccgacggg aggtgccggg caccgtggac
      721 gtagcgtgac ggcatgttcc tcggagagga cctgctcgcc tacctcgtcc tcgccatcgg
      781 cggggccatg gccgtcggct cgatcctggc catcgtccgc ccgccggagc aggtgaagga
      841 cggcgacctg ccggcggcgc cgatcgcccg cagcatcgtc ttcgccgttg tcggcctcat
      901 cggcgcgatc tgggcgctgg ccagcctggt ctccggctga cgacggggcc tacgcctcgg
      961 gctccgggct cgtcgcggtg gccgggttcg gctcggtccg cgtgacccgc aggccgagcg
     1021 actgcagcgt ctccaacggc ttgttgtagc cccgttccag gtggtggacg ccctcgatca
     1081 ccgaggaacc ggtggcgacc gccgcagcga tcacacccga gaagccggcc cggaggtcgg
     1141 ggatctcgac gtgggcgccg tggagctctg acacgccccg gaccacggcg gagtgcacgg
     1201 cgttgccgtc gtggaatcgg cacgccgggc cgccgaggca ggtctcgaac agctcgatct
     1261 cgccgcccat cgcctgcacc accatggccg ggtagcggat gcggtcctcg aacacggtct
     1321 cgtggagcac cgacatgccc tcggcctggg tcatgagcac gaacagcggc gactgccagt
     1381 cggtcatgaa cccgggatgg gtgtcggtgt ggatggcgat ggcacgaagg ccgttcggcg
     1441 cgttggcgct gatccagtcg tcgttgatct cgagctcggc gcccatgcgc tgcagcgtgg
     1501 agatggcggt gaccatccgc tcctgggcgc agcccgccac ccgaacctgg ccgccggtga
     1561 cgaggccggc caccaggtag gagaaggcct cgaggcggtc gccctggagc cgggtggtgg
     1621 ccccgtgcag cttctcgacg ccgtcgacga cgaagcggcg accgggcgtg agctccaccc
     1681 gcgcgcccat gcgctgcagg aacagggcca gctcgatgat ctcgggctcg gtcgccgcgt
     1741 tgttcagcac cgtgcggccc tcggccagcg ccgccgtgag cagcacggtc tccgtggcgc
     1801 cgaccgacgg gtacggcagg gtgatctggg tgcccaccag gcgaccggcc accttggccc
     1861 gcacgccgtc ctcggtctcg gtgacctcgg cgccgaacgc gcggagcgcg tcgacgtgga
     1921 agtcgaccgg gcgggcgccg atgcggtcgc cgccgaccac ggggacgaag gcctcctggt
     1981 ggaggtgcag cagcgggccg agcagcagga tggggatgcg gttgagcccc gagtagcgca
     2041 gggggacctc ggggtcgatc tcgtcgcccg gggtgaccgt gatcgtgtcc ccgtcgaggt
     2101 cgacgccgac gccgagcgac tgcagcacgt cggtggtgat cccgacgtcg ccgacgtcgg
     2161 ggacgttgtg gatcgtggac ggcgagctgc ccatgaccgc agcgaccatg tgcttggtga
     2221 tggcgttctt cgacccggcg acgcggacct cgccgcggag cggcccggag ggctccacgg
     2281 accaggcttc ggcgggagca tccggctcgg cgggggtcat gcagcgagtg tacggccggc
     2341 cgtcgtgccc cctcccggcc ggagcgcttc tggcagcggc cacggaacgc cggtcagcgg
     2401 gcggcgtcga ggtcggcgtt catggcgtcc agagcggtgc gtgccgcggc gccgatgtcg
     2461 tcggtgccgg cctcccgcca ggcgccggtg atgccacggc tggacgacac gagcacgccc
     2521 cggccgtcgg gtcgccgccc ggcgacggca tccgacgccg acgcgccctg ggcgccgtag
     2581 ccgggcacga ggaagacggt gtcgggcatg cgctcccgga gcgcgatcgc gtggtcgggg
     2641 taggtggcgc cgaccaccgc gccgacgtcg ctcagaccgc acgcgcccaa ccggtcggcc
     2701 ccccaggtgg cgacccggtc ggccatccgg tcggcgaccg tgccgccgcc ggcgagctcg
     2761 acgtcctgca gctcgccgct ggcggggttc gacgtcttca ccagcacgaa gacgccaccg
     2821 ccggcggcca gcacgggctc cacgccgtcg gacccgaggt agccgttgac cgtcagccag
     2881 tcggcgccga tgccggcgca cgtcccgccg tcgaggtcgg gagcgccgcc gaaggccatc
     2941 tggccgtagt gggcggccgt ggatccgatg tccccgcgct tggcgtcgag gatcacgggg
     3001 atgccggcct cggtcgcgtg ccgcacggtg gcctcgagcg cggcccaccc ggccaacccg
     3061 taggcctcgt agcaggccgc ttgcggcttc accgccgcgc accgatcggc cacggcgtcg
     3121 atcacccgtt ggttcgcctc gatgaacgcc gacgccactg catccatcgc cccgccggtg
     3181 gtgcggtggc gggcgatgag ctcgccgggc aggaggtcgg gtcgggggtc gaggccgacg
     3241 caggcgatgg aacccgtggc ggtgatccgg gcatcgaggg cgtcggcggg gtgcacggat
     3301 cgaccgtagc ggcaggccga gtcggtgaag ggagccggtt ctgccgacat ttccggcact
     3361 tgtcgcggga cgtgctcaag gcggcgagcg ttccggccga tgactcccgt gagttcgcgc
     3421 aacgaatgac cgccggagac cccgtgacgc cgtccaacga gaagccgacc acgagccgca
     3481 cgcgccgccg tcgtcgcttc gggctgcgcc gacacggcga cggcgacgaa cgaggagcgg
     3541 cgctggtcga gttcgcactc atcgcgacgc cgctgttcct catcctcttc gggaccatcg
     3601 agttcggctg ggcgttcttc cagctcaacg acatccgtca cggtgcacgg gaaggcattc
     3661 gactggtggc cgtcgactcg gatgtgacac cgacgtacac gggttccga
//
LOCUS       DATISU010000356         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_866130, whole genome shotgun sequence.
ACCESSION   DATISU010000356 DATISU010000000
VERSION     DATISU010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866130"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VLR27_14145"
     CDS             <1..544
                     /locus_tag="VLR27_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HSP04648.1"
                     /translation="LQGVQGITDLITTPGLINTDFADVKMIMSNAGSALMGIGYASGE
                     GRALQAARNAISSPLLEASIEGARGILLTISGGSDLGLFEVNEAAEVIHGVAHPEANI
                     IFGTVVDDDMGDEVRVTVIAAGFDRFDGEAPAPRSSGQRDPLRPSSSGGDLGDVLGGD
                     DDDLFGDDGDDDFDVPSFLK"
     gene            567..1238
                     /locus_tag="VLR27_14150"
     CDS             567..1238
                     /locus_tag="VLR27_14150"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HSP04649.1"
                     /translation="MPRSVRSSFSERGDGDLRIDADPAELAARRQAVAPGEWTWLRQV
                     HGARVVEVITPGEHAGAEADAAVTAIPGAVLAVHTADCAPVLLESDDAAVVGAAHAGW
                     RGLVAGVLEQTVASMEGLGATGIRARMGPHIRGRCYEFGTDELELVAARYGDDVRATT
                     AWGTPALDVTAAVRGALGSVGVEVSDRGGCTACEPHRLWSHRARGDRARMAATIAIVE
                     PGTAA"
     gene            1235..1939
                     /locus_tag="VLR27_14155"
     CDS             1235..1939
                     /locus_tag="VLR27_14155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HSP04650.1"
                     /translation="MSIDAADVTAALARVRDRVADAGGDPDAITVVAVTKGFGPEAPV
                     AALRAGLVELGENYAQELQDKADAVAAAGDLPEPSWHFIGRLQSNKVRLVADRVACWQ
                     SLDRASVVDEVAKRAPGARALIQVNAAAEDGKGGASLADAPDLVARARDGGLDVVGLM
                     AVGVAGDEAATESAFAATASLADDLDLPVRSLGMSGDLAAAVRSGTTMIRVGTALFGP
                     RPTPGGSRHTRPTVRD"
     gene            1962..2555
                     /locus_tag="VLR27_14160"
     CDS             1962..2555
                     /locus_tag="VLR27_14160"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HSP04651.1"
                     /translation="MAGAFQKAMVWLGLAPDEEYDDYRYEDQARPAPGGPTRQGGGWG
                     GQQGQGGPRGHQPPPEPEEPVGAYGRGPGGRPTGAVRPLPSRNQPEPPPRAPQREETP
                     TVRPMRPTSAKPTVVAPTSFDAAKDVADRFKANQPVVMDLSDADRDLARRLIDFSSGM
                     CYALGGNMERVTPGVYLITPSGVEVSAEERRRLAGGR"
     gene            2568..2810
                     /locus_tag="VLR27_14165"
     CDS             2568..2810
                     /locus_tag="VLR27_14165"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HSP04652.1"
                     /translation="MGLICSLLQLYVIVIIAGALLSWFPTEPGGGLHQVRMGIARVTD
                     PVMLPVRRAIGTSFGGIDFSPLIVIIGLQLLAGIIC"
     gene            2911..>3308
                     /locus_tag="VLR27_14170"
     CDS             2911..>3308
                     /locus_tag="VLR27_14170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HSP04653.1"
                     /translation="MDLTPDLLLEAKFAPARRGYDMNEVDDFLERCAEGLDVLLARLR
                     AEFDRAERAEAALVELRATGAAPAVDALTETVPVPDTERVPEPQAEAAPASTTSSDPS
                     VGDEASSAPPSSSASASGASPTSPVGGVASP"
BASE COUNT          412 a         1296 c         1175 g          425 t
ORIGIN      
        1 gctccagggc gtccagggca tcaccgacct catcaccacg ccgggcctga tcaacaccga
       61 cttcgccgac gtgaagatga tcatgtccaa cgccggctcg gcgctcatgg gcatcggcta
      121 cgcctccggc gagggccggg cgctgcaggc ggctcgcaac gccatctcct ccccgctgct
      181 cgaggcctcc atcgaaggtg cccgcggcat cctgctcacc atctccggtg gcagcgacct
      241 cggcctgttc gaggtcaacg aggccgccga ggtcatccac ggcgtcgccc accccgaggc
      301 caacatcatc ttcggcaccg tggtcgacga cgacatgggc gacgaggtgc gcgtcaccgt
      361 catcgccgcc ggcttcgacc gcttcgacgg cgaggcaccc gccccccgca gctcgggcca
      421 gcgcgacccg ctgcgcccgt cctcctcggg cggcgacctc ggcgacgtgc tcggtggcga
      481 cgacgacgac ctcttcggcg acgacggcga cgacgacttc gacgtcccgt cgttcctcaa
      541 gtaaccccac ggaccgtccc gtcccggtgc cccgctccgt ccgctcctcg ttctccgagc
      601 gcggcgacgg cgacctccgc atcgacgcgg accccgccga gctggcggcc cgccgccagg
      661 cggtcgcgcc gggggagtgg acgtggctgc gccaggtcca cggcgcccgg gtcgtcgagg
      721 tcatcacgcc gggcgagcac gccggcgccg aggccgacgc tgcggtgacc gccatccccg
      781 gcgccgtgct cgccgtccac accgccgact gcgccccggt gctgctcgag agcgacgacg
      841 ccgcggtcgt cggcgcggcc cacgccgggt ggcggggcct cgtcgccggc gtgctcgagc
      901 agaccgttgc gtcgatggag ggcctcggcg ccactggcat ccgggcccgg atggggccgc
      961 acatccgggg ccgctgctac gagttcggca ccgacgagct cgagctggtc gcggcccgct
     1021 acggcgacga cgtgcgggcc accaccgcct ggggcacgcc agccctcgac gtgaccgccg
     1081 ccgtccgggg tgccctcggc tcggtcgggg tcgaggtgtc ggaccggggc gggtgcacgg
     1141 cgtgcgaacc gcaccggctg tggtcccacc gcgcccgggg cgaccgggcc cgcatggccg
     1201 ccaccatcgc catcgtcgag ccggggacgg cggcgtgagc atcgacgccg ccgacgtcac
     1261 cgctgcgctg gcgcgggtcc gcgaccgggt cgccgacgcc ggcggggatc ccgacgccat
     1321 caccgtggtc gccgtcacca agggcttcgg cccggaggcc ccggtcgccg cgctgcgtgc
     1381 cggcctcgtc gagctgggcg agaactacgc ccaggagctg caggacaagg ccgacgccgt
     1441 cgccgccgcg ggggacctgc ccgagccgag ctggcacttc atcggccgcc tccagtcgaa
     1501 caaggtgcgc ctcgtggccg accgggtcgc gtgctggcag agcctcgacc gggcctcggt
     1561 ggtcgacgag gtcgccaagc gtgcgcccgg tgcccgggcc ctgatccagg tgaacgccgc
     1621 cgccgaggac ggcaagggcg gcgcctccct cgccgacgcg cccgacctcg tggcccgggc
     1681 ccgggacggc ggcctcgacg tcgtcggcct gatggccgtc ggcgtggccg gagacgaggc
     1741 cgcgaccgag tcggccttcg ccgccacggc ctcgctcgcc gacgacctgg acctccccgt
     1801 gcggtcgctg ggcatgagcg gcgatctcgc ggcggcggtc cgctccggca cgacgatgat
     1861 ccgcgtgggt acggccctgt tcggaccccg acccacccct ggcggttcgc ggcacacgcg
     1921 accgaccgtg cgagactgac cgacgagacg aggaggcacc gatggctggc gcgttccaga
     1981 aggccatggt ctggttgggc ctggcgcccg acgaggagta cgacgactac cgctacgagg
     2041 accaggcgcg cccggcgccc ggcggcccca cccgccaggg cggcggctgg ggcggccagc
     2101 agggccaggg cggtccccgt ggccaccagc ccccacccga gcccgaggag cccgttggcg
     2161 cctacggccg cgggccggga ggccgtccca ccggtgcggt ccgtccgctg ccgtcgcgga
     2221 accagcccga gccgccaccg cgcgcgcccc agcgcgagga gacgcctacg gtgcgcccca
     2281 tgcgccccac ctcggccaag cccaccgtcg tcgccccgac gtcgttcgac gcggccaagg
     2341 acgtcgccga ccggttcaag gccaaccagc ccgtcgtgat ggacctctcc gatgccgatc
     2401 gcgacctggc ccgccggctc atcgacttct cctcgggcat gtgctacgcc ctcggcggca
     2461 acatggagcg cgtcacgccc ggcgtgtacc tgatcacgcc gtccggcgtc gaggtgtccg
     2521 ccgaagagcg gcgacgcctc gccggcggcc gctgacgagg accgaccgtg ggcctgatct
     2581 gctcgctgct gcagctgtac gtcatcgtca tcatcgccgg ggcgctgctg tcgtggttcc
     2641 ccaccgagcc cggcggcggc ctgcaccagg tgcgcatggg catcgcccgg gtcaccgatc
     2701 cggtgatgct cccggtccgc cgggccatcg gcacgagctt cggcgggatc gacttctcgc
     2761 cgctcatcgt gatcatcggc ctgcagctgc tcgccggcat catctgctag caccgctcgg
     2821 ggcccggcac cccggtcccc gcttctggca gtgctggtgt agcgccacca tcacccagtc
     2881 ctgacggcgg ggacgcgtag catcgtggcc atggatctga cgcccgatct gctcctcgag
     2941 gccaagttcg ctcccgctcg ccggggctac gacatgaacg aggtcgacga cttcctcgag
     3001 cgctgcgccg agggactcga tgtcctcctg gctcgcctcc gggccgagtt cgaccgggcc
     3061 gagcgggccg aggccgcgct ggtcgagctg cgtgccaccg gggctgcgcc ggcggtcgac
     3121 gccctgaccg agaccgtgcc ggtgcccgac accgagcgcg tgcccgagcc ccaggcggag
     3181 gctgcaccgg cgtcgaccac gtcgtcggac ccgtcggtcg gggacgaggc gtcatctgcg
     3241 ccgccgtcga gctccgcgtc ggcgtcggga gcatcgccga cgtcgccggt gggcggcgtg
     3301 gcgagccc
//
LOCUS       DATISU010000357        19213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_307286, whole genome shotgun sequence.
ACCESSION   DATISU010000357 DATISU010000000
VERSION     DATISU010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 19213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19213
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307286"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VLR27_14175"
     CDS             complement(<1..416)
                     /locus_tag="VLR27_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HSP04654.1"
                     /translation="MDGGDAWVLMSTALVLFMVPGLALFYGGMVRSKNVLNMLLMNLY
                     CLAVIPLLWVTVGASLSGSGSNGIIGGFDNIGLKGLDGDGLLATAFLMTFAAITPALI
                     SGAVADRMRFSTWAVFVPLWSLIVYVPVVFWIYGLDA"
     gene            complement(527..1483)
                     /locus_tag="VLR27_14180"
     CDS             complement(527..1483)
                     /locus_tag="VLR27_14180"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HSP04655.1"
                     /translation="MRADRLVAVLLLLQQRGRVTAREVADELEISERTARRDLEALGL
                     AGIPVYSQAGRGGGWRLVGGARTDLSGLTAAEARALFLVAGPASGGAPAVRSALRKLV
                     GALPEPFRVEAEAAAASLHVDNVAWGRAGDATVPAFLDPLQQAVIDGVQVELGYVARD
                     GRSSSRTVHPLGVVAKGPSWYLVAGTDRGQRTFRIDRVTDVTRTNRPAERPDGFDLAA
                     EWRAIAEAIDRSGTPIEIRAVADPDHVDVLRWILGSRLDVGGPGPGGVEVVIRGHSSR
                     SIAGELAGLGSWLHVTSPEEVRDELADIGQQLRDLYGGDRTS"
     gene            1546..2211
                     /locus_tag="VLR27_14185"
     CDS             1546..2211
                     /locus_tag="VLR27_14185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HSP04656.1"
                     /translation="MEPTTATTTTPAPFGVDDPRHVLARAVAVGTATVVAVRPDQLDG
                     PTPCSEMDVRHLLGHLVSVLDRIAALGRGEDSFAVVESADHDDWPGAWAAAAHRVQAA
                     WTDDDVLARPMALPWQQGTGADILLGYVNEVVVHTWDLAVATGQRPVWDDDVVTVALE
                     RMPALPAEDRLAMFEQISADMGLPEVGVPFAEAVPVGADAAAIDRLVAWNGRDPRWAP
                     ARG"
     gene            2240..2917
                     /gene="queC"
                     /locus_tag="VLR27_14190"
     CDS             2240..2917
                     /gene="queC"
                     /locus_tag="VLR27_14190"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HSP04657.1"
                     /translation="MTRPAVVLLSGGLDSATCLAIAADEGLAPHAMSFRYGQAHTAEL
                     DAARRVASALGAVEHRLVDIDLAAFGGSSLLGDGEIPKGREEIGEGIPSTYVPARNTV
                     FLAYALAWAEVLGANDIFIGVNALDYSGYPDCRPEFITAFEAAANAGTRTGTEGDGYR
                     IRAPLIDLTKAEIIRLGTGLGVDYAMTVTCYDATADGACGECDACVLRRRGFEEAGVP
                     DPTSYRP"
     gene            2914..3549
                     /gene="queE"
                     /locus_tag="VLR27_14195"
     CDS             2914..3549
                     /gene="queE"
                     /locus_tag="VLR27_14195"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR04508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HSP04658.1"
                     /translation="MSTYAVKEVFRTLQGEGRQAGRAAVFLRFSRCNLWSGHERDRAT
                     AVCRFCDTDFVGTDGPGGGVFRGATELAVHVAETWGAGREHRFVVCTGGEPALQLDDE
                     LVEALHAEGFEVAVETNGTLPLAAGIDWVCVSPKAGTDLAVTAGDELKVVWPQDGTDL
                     DALEALDFDHLLVSPMDGPDRAAATAAAVALCLERPRWRLSLQTHKVLGIP"
     gene            3645..3947
                     /locus_tag="VLR27_14200"
     CDS             3645..3947
                     /locus_tag="VLR27_14200"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HSP04659.1"
                     /translation="MDTVTGHPLYDADGHKLGKIVDVLGLYGGADDIGWLAVKVGLRG
                     TKLVPNTGVEPGEDGFTTPFTKDQITSAPKVPPHFEPAGDDREALLSHYGVRLAGL"
     gene            4081..4860
                     /locus_tag="VLR27_14205"
     CDS             4081..4860
                     /locus_tag="VLR27_14205"
                     /inference="COORDINATES: protein motif:HMM:NF039623.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HSP04660.1"
                     /translation="MPDPGGLPAGSIDDAIARMDRIIARCAETGDARGWFAVVYRAVT
                     ARVRDGIAAGAFDDGERMEAFDVRFATHWLDAYDAWDRARPVSASWACSFEAAARPGV
                     ILQHLLLGMNAHINLDLGLAAAQTCPGPGVVELRDDFERINDVLGELVDAMQAAISEV
                     SPMARVLDVVGLRLDEAIVSFSLAQARARSWRFAEELATADLAPALVDARDAAVAGYG
                     ARLARPGFAMRCLLPIARWREEPDLARVAGLLRARGAEVQL"
     gene            4914..5621
                     /locus_tag="VLR27_14210"
     CDS             4914..5621
                     /locus_tag="VLR27_14210"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSP04661.1"
                     /translation="MSDRPRKYRQSRFVRPPGACEVILVRHGESQPADPDEPFPLVDG
                     QGDPPLHEPDGVEQARLACERLLASGEEFAAVYVTTLQRTRQTAQVLLDRLGLEPVVE
                     PDLREVHLGEWEGGLFRKKVAEGDPIAEQMYLEQRWDVIPGAESSEDFAGRVRRALDG
                     IARTHPDQTVAVFTHGGVIGQVMAEASGARGFAFTGSDNCGITHVVIANDRWIVRTWN
                     DTSHLSPRFTHDPEPLI"
     gene            5618..5983
                     /gene="queD"
                     /locus_tag="VLR27_14215"
     CDS             5618..5983
                     /gene="queD"
                     /locus_tag="VLR27_14215"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HSP04662.1"
                     /translation="MTMELFKEFGFESAHSLPNVPDGHKCARLHGHSFRVRVTVEGAV
                     GDDSGWVMDFADLKAAVQPTIDALDHRLLNDIEGLENPTSEVLAVWIWDRVAPGLPGL
                     AAVEVRETCTSGCTYRGGE"
     gene            5994..6389
                     /gene="queF"
                     /locus_tag="VLR27_14220"
     CDS             5994..6389
                     /gene="queF"
                     /locus_tag="VLR27_14220"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HSP04663.1"
                     /translation="MSDAPTDDLTLLGQPTRGPVEHVETFPAPEGCTRVVFTTDEVAS
                     LCPVTRQPDISSVRIDYVPDQRCIESKSLKLFLWGFRDRHVFAEALAVEIADEIWETV
                     EPESVDVVVTQHVRGGIVTETHSSHPRPS"
     gene            complement(6382..7026)
                     /locus_tag="VLR27_14225"
     CDS             complement(6382..7026)
                     /locus_tag="VLR27_14225"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HSP04664.1"
                     /translation="MPSPTDRRFDAVVFDFSGVMVSSAFDAIARHAGGHGMSEDEFLE
                     FLLGPYAEDSDHAWHRAERGEIGILDWATDTMARAEEAGMGLDLSFMAGMLGDLEVYE
                     VMVDAARTLRVDGYRTSLLTNNIAEGRDSWRPLLPLDDLFDDVIDSSEVGLRKPNPEI
                     YLLALARLGGIEPSRAVMLDDHPGNCAGALAAGMQAIQVRDPAEAVEELRALLS"
     gene            7079..8263
                     /locus_tag="VLR27_14230"
     CDS             7079..8263
                     /locus_tag="VLR27_14230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP04665.1"
                     /translation="MTSSPNPVRVVASGSVALTTTVVPAFLTGALSGRIGDDLGFGDG
                     GAGAAVAAFFVGAGVFAVPMGRVTERIGPTRALRAGALVSAAAALAIAAFASSLWELV
                     ALLVVAGSAVGLVDTGAARAFGDSVPSTRHGIAFGAKEASVPAASLAAGLSIPVLAEQ
                     FGWRSAFVAGALLAPLVWVLVPRMAADRPPARDGRAGDSRRGPLVLFAAGVALGVGAA
                     TAASTLLVPAIEDRGWDEADAGLLLAVASSASIAVRLAAGSRGDRRPLSTWAMVATLL
                     GVGAVGAVLLAATSAGPAGVIGAIAVIGGGWGWTGLAFQTAVRVGRDRPAAAAGVVLT
                     GLSVGGAAGPAAFGAIASALSYSAAWATAAVALVAGGAAILASKHWLESGSAQERTTA
                     AR"
     gene            8285..9211
                     /locus_tag="VLR27_14235"
     CDS             8285..9211
                     /locus_tag="VLR27_14235"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP04666.1"
                     /translation="MALDPQAKALLDALAQMEGPPILESEPAEVREAMAALGAMSDAS
                     AEEVREVVEQTIPGPGGDLRVRIYTPVLATEGAPQACLVWYHGGGWVIGDLDTTDGVC
                     RALCGRSAATVISVDYRLAPEHPYPAALDDAEAAFDWVRDHADDLYIAPDRIAVGGDS
                     AGGNLAALVAQSRRGQVAFQLLVYPATDLTNSHPSHEENAEGYLLTRDHMIWFTQHYL
                     GDHDRTDPRVSPLHADDLSGVAPAFVLTAEFDPLRDEGEAYAARLEDAGVEVTSSRYD
                     GMIHAFFQLSGAIDRANDALDEAAAALRRALT"
     gene            9208..9417
                     /locus_tag="VLR27_14240"
     CDS             9208..9417
                     /locus_tag="VLR27_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04667.1"
                     /translation="MKHRLATLAVALVLVLGACESDQDPGIDPGVTDGPAETSDTLGT
                     CPAGGPDDTTPPAGCLGPDGSVLRP"
     gene            complement(9433..10518)
                     /locus_tag="VLR27_14245"
     CDS             complement(9433..10518)
                     /locus_tag="VLR27_14245"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HSP04668.1"
                     /translation="MTLPDDVAARPLVPLPGQPDGVPWPTHEWPTGEEPTDRALAPLL
                     DVVMDPDSGHGRTRAVVVVHRGRLVAERYGDRTDAWGEVTGDPVDASTPLLSWSMAKS
                     MLHAVVGMLVDEGRLRLDDAAPVPAWDEGDPRAAITLEQLLAMRDGLDFAEDYAPDDQ
                     AGAVSHVIEMLFGSGASDVAGFAASRPLAHEPGTVFNYSSGTSNIISGVVARALGGEE
                     PYRRFLHERLFDPPGMRTADPRFDDAGTWVASSYVWATARDMARFGYLYLRDGVWDGT
                     RLLPEGWVDHGRRPRSVDPVDGRLYGAQWWVVGDDVGTFWANGYEGQSIMVCPGLDLV
                     VVRLGKSTAECYPALIEWRAAVVDAFR"
     gene            complement(10556..10987)
                     /locus_tag="VLR27_14250"
     CDS             complement(10556..10987)
                     /locus_tag="VLR27_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSP04669.1"
                     /translation="MLVDQILEAKGRAVATVAPDATVAEAVAALAEHNVGALVVSSDG
                     KTVEGIVSERDVVRGLGESAEVLQRRVRDLMATDVSTCTGRSGVEELMTTMTTGRFRH
                     LPVVADGELCGIISIGDVVKARIDELATETQQLVGYIRDGR"
     gene            complement(11053..12381)
                     /locus_tag="VLR27_14255"
     CDS             complement(11053..12381)
                     /locus_tag="VLR27_14255"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HSP04670.1"
                     /translation="MPFPRPRSRARRGLGAFVVAVVLGCAWWAGPAGAQEVPTHPGSL
                     GPAPTHAFVQRVHQLFLGRAATPAEATELAHLMHVDGPAALTGRLALSPEWAGVRIDD
                     LYRTILGRSPDPGGQAYWLDQLRRGRTLESVAAGFYGSDEYYGAVGGRADAFVDSLYR
                     RLLEREPDAGGRQHWLGHMWAGMSRMAVAGSFYGSLESRRQRVDALYGEILGRAPDPG
                     GRAFWVERLPALGDVALASHLAASDELHGRATGVRLPDVRVEGVGAGTRYPLSASWRP
                     GCPVGPGDLRAVTFPHFRTDGSIGRGVLIVHRDAAHDVAVLVRTAYGSRFPITQARPV
                     DDFGGDDDASMAADNTSAFNCRVVAGTTTWSQHAYGRAVDLNPVRNPYVRGDRVEPPS
                     GGAYVDRSDVRPGMLVEGSAVIRAVDRLGWGWGGRWASGADHQHVSRNGR"
     gene            12446..13042
                     /gene="yihA"
                     /locus_tag="VLR27_14260"
     CDS             12446..13042
                     /gene="yihA"
                     /locus_tag="VLR27_14260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HSP04671.1"
                     /translation="MSKPLALRFVTSADRLDRLPPTAAEVTVVGRSNVGKSSLLNALA
                     NRTNLAKTSKTPGRTQLLNCFELEPHDSVEGTFVDCPGYGYAKTPKSVRQGFQPMIEG
                     YLLGREELRMVGVLVDGEIGPTKLDVLLLEWLRHHDVPHTVIATKHDKVKSKAREKRK
                     RDLAEGCGVEKGDVLWVSAHNGVNIDVLRDRIRGWLAS"
     gene            13039..14298
                     /locus_tag="VLR27_14265"
     CDS             13039..14298
                     /locus_tag="VLR27_14265"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP04672.1"
                     /translation="MTSTTERGEEAGARFFPGWIVVGAAFVLLMANSGFIFYGQAVYL
                     DALTTERGFSTGAASLGQSLVFVVGGLVGPRVGKLITDRDVRVVVLGGLVTSWVAVVL
                     LGRVEELWQLYAVNVLLGVGFSAAGLVPATTVVTRWFETRRSVALAIASTGLSVGGLT
                     FTQLVAWWIRRDGLELATPWLVVVWAACTLPVVPLLWPTPEHRGVLASGAVAGSFAMG
                     VAYDVAVRSRFFTMVTAGYVLVLGMQVGGITHLVKLGNERAGDGTGGRLIAGLTIASV
                     VARLIGGVVADKQPLVRLAVGFAATQALALALLSQVESSLLLVLCAVLFGATIGNILM
                     LQPLLFADAMGARDYARIFALNQLIVVAGVALGPFLLGALHDAASYRASYIVAAVLCG
                     VGTVLLSRAGSTAAARAELDSERPATT"
     gene            14330..14596
                     /locus_tag="VLR27_14270"
     CDS             14330..14596
                     /locus_tag="VLR27_14270"
                     /inference="COORDINATES: protein motif:HMM:NF025715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HSP04673.1"
                     /translation="MDDELRTHLDQLEAKIAEARADGNIDEAEREELRVLVERVRSDL
                     GSGDPDRDGLAEQLEESAVRFESDHPTVAAVIRSAVHTLTGYGM"
     gene            14668..17466
                     /locus_tag="VLR27_14275"
     CDS             14668..17466
                     /locus_tag="VLR27_14275"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HSP04674.1"
                     /translation="MDGSTTDGIASAIRSATGSIVVVGDAHHRAVAATAPVGDAHLVE
                     VRPAPEPTPWSGLAAILDACRSEWPRLPVPAALEAAATGRPVGDHLGLAGDLGSLLAR
                     VGEEGPWTLLVHDAHRFDLSSAAALSRALAQEPAGLRAVVTQDRSGRGVEGIPVHVAS
                     GGAAPIQDGPPLAGPAGIEGAEGRASAALRLGAPLDAAAWAEAAGDWATAAARWLEGG
                     RPDRCRRALGLAEPDPHVAEVTARLAHRTGSERERRIAAQRAIPILDAHDPLASVRLR
                     LLEASARVWSDAVGASATLAVAADLLATGGSSPAARTVRDLHALVVSTAALGRDGDPE
                     PLRCSVERPLARLGAGGIDPDAIHLLTAAARVLAWQGHLDDARSLLDRVVGVLDARRR
                     FLLLAEPLAASAWLARRRGRLELALTHGARALDIARACGWTSDERIATVEVAHIEAMR
                     GRLDDCRRHVATLVPPGTVPRGAAQIGAVSALAVGELLADAPERAVELLEPVQERFGA
                     SISPAEIAWRHNLVEAYVRTGRDADAARVLDDLVGWVARTDSTREQGQVAWCRGMLAP
                     PGEHDADFTEARRLLDAYPTLRWRSDLHHLRRLLDERRHDTAADVAGRLVSEARSAGL
                     LHAVERVHRLQREHGIDVPEPAPSTSVLSVEDLRVALALAEGTEVEEVATLLQLTPRR
                     VEVVRDRVMGLLGVDAPEQLAGFLPQDRATPVPVAAVVCVLGPTVVHRGAQQLVPPAG
                     RPEAVLALVATERDVPVERVLDVLWPDVDPGRSRSRLRNVLSRLRAAAGALVERDGDR
                     LVLSAGVEVDAHRFDQLAELALRAPPDEVLARTDAALAAWHGEPLPAWPYEDWALRER
                     HRLVQRCVALHVRRAEIHAASGAVGAALDELELAVGLQPDARDLWERAVHLAEADERI
                     GRARALRRRAADHDVDLV"
     gene            complement(17477..17641)
                     /locus_tag="VLR27_14280"
     CDS             complement(17477..17641)
                     /locus_tag="VLR27_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04675.1"
                     /translation="MYEHCAAERTRSLLLQAVLVGLLVLGGLTLFDTVSSATEPVAEG
                     MDQRADTPSP"
     gene            complement(17808..19166)
                     /locus_tag="VLR27_14285"
     CDS             complement(17808..19166)
                     /locus_tag="VLR27_14285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSP04676.1"
                     /translation="MSTEHTLDQVTSSPARAEPGSDAMFRAIVQTSVNPFVVLDPAGH
                     VRWCSEKVEMLIGRTADDVIGLHFLDLIDPSSHEAVLAEYAEFTSDDQLRRAWVGPPM
                     LLDMVHADGSRITCEVSAATATTHGIEGIVVQVRRWRGTVLLYAAVDALAAGEPIDDV
                     LTKLAAIVEHDMPGSVVFVAAGWDSDRFDVVVTAPGTASTHRDLAARIAEMPVIDWHG
                     DGTGPVSNPDLADEAGLASCWSIPITVRGDQRPSATLVLLRPMDAPPLPHSTTVERVA
                     TLVALAIESDRNRRAWRRSAVTDQLTELPNRSGLDEWLGAQAVDRADEGLALLFCDVD
                     EFKYVNDHLGHAMGDRTLQVVAERLRRSVRDGDLVCRWGGDEFVVVCAEPAAAEALAR
                     RLIDHVRDPIGLEQHTARVGLSVGVSFGTFATPLDDLLRESDRALLEAKSQGRNRYVV
                     RA"
BASE COUNT         2458 a         7076 c         7091 g         2588 t
ORIGIN      
        1 gcgtcgaggc cgtagatcca gaacacgacg ggcacgtaga cgatgaggct ccacagcggc
       61 acgaacacgg cccaggtcga gaaccgcatg cggtcggcga cggcgccgct gatgagggcc
      121 ggggtgatgg cggcgaaggt catgaggaag gcggtggcga ggaggccgtc gccgtcgagg
      181 cccttcaggc cgatgttgtc gaagccgccg atgatgccgt tggagcccga cccgctcagc
      241 gaggcgccga ccgtgaccca cagcaggggg atgacggcca ggcagtacag gttcatcagg
      301 agcatgttca gcacgttctt cgagcgcacc atgccgccgt agaacagggc caggcccggc
      361 accatgaaca ggaccagcgc ggtcgacatc agcacccagg cgtcgccacc atccatcgca
      421 gttcgctccc tcgtcgcgtg ggggcgacag gtgtcgccca gatcggggtc acgttggcga
      481 aagggtgtgt cccgaggggt tcccgggtgt ttcggcagtg tgaacgtcag gaggtgcggt
      541 cgccgccgta gaggtcgcgc agctgttgcc cgatgtcggc gagctcgtcg cggacctcct
      601 cgggcgaggt gacgtggagc caggagccga ggccggcgag ctcgccggcg atggaccggc
      661 tgctgtggcc gcggatgacg acctccacgc caccgggacc cgggccgccg acgtccagcc
      721 gggatccgag gatccaccgc aggacgtcga cgtggtcggg gtcggcgacg gcgcggatct
      781 cgatcggcgt gccgctccgg tcgatggcct cggcgatggc ccgccactcg gcggccaggt
      841 cgaagccgtc cgggcgctct gccggccggt tcgtgcgggt gacgtcggtg acccggtcga
      901 tccggaacgt ccgctggccc cggtcggtcc cggccaccag gtaccacgac ggtcccttgg
      961 cgacgacgcc gagcgggtgg accgtgcgcg acgagctgcg cccgtctcgg gcgacgtacc
     1021 cgagctcgac ctgcacgccg tcgatcacgg cctgctggag cggatcgagg aaggcgggca
     1081 ccgtggcgtc acccgcccgg ccccacgcga cgttgtccac gtggagcgag gcggccgccg
     1141 cttcggcctc cacccggaac ggctccggca gcgccccgac cagcttgcgc agcgccgagc
     1201 gcacggccgg ggccccaccc gatgccggcc cggcgacgag gaacagcgcc cgggcctcgg
     1261 cggcggtgag gccgctgaga tcggtgcggg cgccgccgac cagcctccag ccgccgcccc
     1321 gcccggcctg ggagtacacc gggatgcccg cgagcccgag ggcctccagg tcccggcggg
     1381 cggtccgctc cgagatctcg agctcgtcgg ccacctcgcg ggcggtgacc cgaccccgtt
     1441 gctggagcag gaggaggacg gcgacgaggc ggtcggcgcg catgccgaga tgttcgcaga
     1501 gaaactggcc aacagatgac cggtttcgac gcggatgctg catccatgga acccaccacc
     1561 gccaccacga ccaccccggc gcccttcggc gtcgacgatc cccgccacgt cctcgctcgg
     1621 gccgtcgctg tcggcacggc gacggtcgtc gcggtccgtc cggaccagct cgacggtccg
     1681 accccgtgct cggagatgga cgtgcgccac ctcctcggcc acctcgtctc cgtgctcgac
     1741 cgcatcgccg ccctcgggcg gggcgaggat tccttcgccg tcgtcgagtc cgccgaccac
     1801 gacgactggc ccggtgcctg ggcggcggcc gcgcaccgcg tgcaggcggc ctggaccgac
     1861 gacgacgtgc tcgcccgccc catggcgctg ccgtggcagc agggcacggg cgccgacatc
     1921 ctcctcggct acgtgaacga ggtcgtcgtc cacacgtggg acctggcggt ggccaccggc
     1981 cagcgcccgg tgtgggacga cgacgtcgtc accgtggcgc tcgagcggat gcccgccctc
     2041 ccggccgagg atcgccttgc catgttcgag cagatctccg ccgacatggg tctgcccgag
     2101 gtcggcgtcc ccttcgccga ggcggtgccc gtcggcgccg atgcggcggc gatcgaccgg
     2161 ctggtcgcct ggaacggccg cgatccgcgc tgggcgcccg cccgaggctg accgccggcg
     2221 gcgcccgtag gttggagggg tgacccgacc tgccgtcgtc ctgctctccg gtggcctcga
     2281 ctcggccacc tgcctggcga tcgccgccga tgagggcctg gcaccccacg cgatgagctt
     2341 ccgctacggc caggcccaca ccgccgagct cgacgccgcc cgtcgggtgg cgtcggcgct
     2401 cggcgcggtc gagcaccgcc tcgtggacat cgacctcgcg gccttcggcg ggtcgtccct
     2461 gctcggcgac ggcgagatcc cgaaggggcg cgaggagatc ggcgagggca tcccctccac
     2521 ctacgtgccg gcccgcaaca ccgtgttcct cgcgtatgcg ctcgcgtggg ccgaggtgct
     2581 cggggccaac gacatcttca tcggggtcaa cgccctcgac tactcgggct acccggactg
     2641 ccggccggag ttcatcaccg cgttcgaggc ggccgccaac gccggcaccc gcacgggcac
     2701 cgagggggac ggctaccgga tccgggcccc gctgatcgac ctgaccaagg ccgagatcat
     2761 ccgcctcggc accgggctcg gcgtggacta cgccatgacg gtcacctgct acgacgccac
     2821 cgcggacggc gcctgcggcg agtgcgacgc gtgcgtgctg cgtcgccggg gcttcgagga
     2881 ggcgggcgtg cccgacccga cctcgtaccg gccatgagca cctacgcagt caaggaggtg
     2941 ttccgcaccc tgcagggtga gggccgccag gccgggcgcg ctgcggtgtt cctgcggttc
     3001 tcccgctgca acctgtggtc cgggcacgag cgcgaccggg ccacggcggt gtgccggttc
     3061 tgcgacaccg acttcgtcgg cacggacgga cccggcgggg gtgtgttccg cggggccacc
     3121 gagctggccg tccacgtcgc cgagacctgg ggcgcaggtc gcgagcaccg cttcgtggtg
     3181 tgcacgggcg gcgagccggc gctgcagctc gacgacgagc tggtcgaggc gctgcacgcc
     3241 gagggcttcg aggtcgcagt ggagaccaac gggacactcc cgctggccgc aggcatcgac
     3301 tgggtgtgcg tgagccccaa ggccggcacc gacctcgccg tcaccgccgg cgacgagctc
     3361 aaggtcgtgt ggccccagga cggcaccgac ctcgacgccc tcgaggcgct cgacttcgac
     3421 cacctgctgg tctcgcccat ggacggcccg gaccgagcgg ccgccacggc cgccgcggtc
     3481 gcgctctgcc tcgagcgccc ccgctggcgc ctcagcctcc agacccacaa ggtgctcggc
     3541 atcccgtgag ggcccgagcg gaagaaaata ttgcgaacgc gtgacaaacg gtcatgtcgc
     3601 tgttatgttg atccctgggc ggcaccttca gaggaggagc tgacatggac acggtcaccg
     3661 gacatccgct gtacgacgca gacgggcaca agctggggaa gatcgtcgac gtgctcggcc
     3721 tctacggcgg tgccgatgac atcggttggc tggccgtcaa ggtcgggctc cgcggcacga
     3781 agctcgtgcc caacaccggc gtcgagcccg gcgaggacgg cttcaccacg ccgttcacga
     3841 aggatcagat caccagcgcc cccaaggtcc cgccgcactt cgagccggcc ggcgacgacc
     3901 gcgaggcgct cctttcccac tacggcgtcc ggttggctgg cctgtgaccc cgtagggacc
     3961 cggtgccccc gatcgcgctg gtcggggggc accgggatgc tcttcatcaa cagagacggt
     4021 tgacgacccg gcgcctggcg ccgggtcgtc gtcgtgtgcg ggcgatgcca gactcgggcc
     4081 gtgccggacc cgggcgggct ccccgcggga agcatcgacg acgccatcgc gcgcatggac
     4141 cggatcatcg cccggtgcgc cgagaccggt gacgcgcgcg ggtggttcgc ggtggtgtac
     4201 cgggcggtca cggcgcgggt ccgcgacggg atcgccgccg gtgcgttcga cgacggcgag
     4261 cgcatggagg ccttcgacgt ccgcttcgcc acccactggc tggatgccta cgacgcgtgg
     4321 gatcgggcga ggccggtgtc ggcctcgtgg gcgtgctcgt tcgaggcggc cgcccggccc
     4381 ggggtgatcc tccagcacct gctcctcggc atgaacgccc acatcaacct cgacctcggc
     4441 ctggccgccg cccagacctg cccgggaccc ggcgtcgtcg agctccgcga cgacttcgag
     4501 cgcatcaacg acgtgctcgg cgagctcgtg gacgcgatgc aggccgcgat cagcgaggtg
     4561 tcaccgatgg ctcgcgtgct cgacgtggtg gggctccggc tcgacgaggc gatcgtgagc
     4621 ttctcgctcg cccaggcgcg cgcccggtcg tggcgcttcg ccgaggagct cgccactgca
     4681 gatctggctc cggcgctcgt cgatgcccgc gacgccgcgg tggcggggta cggcgcccgg
     4741 ctggcccgac caggcttcgc catgcgctgc ctgctgccga tcgcccggtg gcgagaggag
     4801 ccggatctcg cccgggtcgc tggtctgctc cgggctcggg gtgccgaggt ccagctctga
     4861 cgtcgccgga tcggagtcgc ccgcatcccg aggggctctc taccgtggcc gccgtgtccg
     4921 atcgccctcg caagtaccgc cagagccgct tcgtgcgccc gcccggggcg tgcgaggtca
     4981 tcctcgtgcg ccacggcgag tcccagccgg ccgaccccga cgagcccttc cccctggtcg
     5041 acggccaggg cgacccgccg ctgcacgagc ccgacggggt cgagcaggcc cgcctggcgt
     5101 gcgagcggct gctggcctcg ggggaggagt tcgccgccgt ctacgtgacc acgctgcagc
     5161 ggacccgcca gaccgcccag gtgctgctcg accgcctcgg cctcgagccc gtggtcgaac
     5221 ccgacctgcg cgaggtacac ctcggcgagt gggagggcgg cctgttccgc aagaaggtgg
     5281 ccgagggcga ccccatcgcc gagcagatgt acctcgagca gcgctgggac gtgatccccg
     5341 gtgccgagtc cagcgaggac ttcgccggcc gcgtccgtcg ggcgctcgac gggatcgccc
     5401 ggacccaccc ggaccagacc gtggccgtgt tcacccacgg cggcgtcatc ggtcaggtca
     5461 tggccgaggc cagcggcgct cggggcttcg ccttcaccgg ctccgacaac tgcggcatca
     5521 cccacgtcgt gatcgccaac gaccggtgga tcgtgcggac ctggaacgac acgagccacc
     5581 tcagcccgcg cttcacccac gaccccgaac cgctcatctg acgatggagc tgttcaagga
     5641 gttcgggttc gagtcggccc actcgctgcc gaacgtgccc gacggccaca agtgcgcccg
     5701 cctgcacggc cacagcttcc gggtgcgggt caccgtcgag ggcgcggtgg gcgacgacag
     5761 cggctgggtg atggacttcg ccgacctcaa ggccgcggtg cagcccacca tcgacgcgct
     5821 ggaccaccgc ctcctcaacg acatcgaggg gctggagaac ccgaccagcg aggtgctggc
     5881 cgtgtggatc tgggaccggg tcgcgcccgg cctcccgggc ctcgccgcgg tcgaggtgcg
     5941 cgagacatgc accagcggct gcacctaccg gggtggggaa tagcctggcg tcgatgagcg
     6001 acgccccgac cgacgacctc accctcctcg gccaaccgac ccgcgggccc gtcgagcacg
     6061 tcgagacgtt cccggcgccg gagggctgca cccgggtggt cttcaccacc gacgaggtgg
     6121 cgtcgctgtg cccggtcacc cgccagcccg acatctcctc ggtgcggatc gactacgtcc
     6181 ccgaccagcg ctgcatcgag tccaagtcgc tgaagctgtt cctgtggggc ttccgcgacc
     6241 ggcacgtctt cgccgaggcg ctcgccgtgg agatcgccga cgagatctgg gagaccgtgg
     6301 agcccgaatc ggtcgacgtc gtcgtgaccc agcacgtgcg cggcggcatc gtcaccgaga
     6361 cgcactcgtc ccacccccgt cctagctgag cagggcgcgc agctcctcca ctgcttcggc
     6421 cgggtcgcgc acctggatcg cctgcatgcc ggcggcgagc gcgccggcgc agttgcccgg
     6481 gtggtcgtcg agcatcacgg cgcgtgacgg ctcgatgccg ccgagccggg ccagggccag
     6541 caggtagatc tccgggttgg gcttgcgcag cccgacctcg gaggagtcga tgacgtcgtc
     6601 gaacaggtcg tcgaggggga gcagcgggcg ccacgagtcc cggccctcgg cgatgttgtt
     6661 ggtgagcagc gaggtgcggt acccgtcgac gcgcagcgtg cgcgctgcat cgaccatgac
     6721 ctcgtagacc tcgaggtcgc cgagcatgcc ggccatgaag gacaggtcga ggcccatgcc
     6781 ggcctcctcg gcgcgggcca tcgtgtccgt ggcccagtcg aggatgccga tctcgccccg
     6841 ctcggcgcgg tgccaggcgt ggtcgctgtc ctcggcgtag gggccgagga ggaactcgag
     6901 gaactcgtcc tcgctcatcc cgtgcccgcc ggcgtggcgg gcgatggcgt cgaaggccga
     6961 gctcaccatc acgcccgaga agtcgaagac gacggcgtcg aagcggcggt ccgtcgggct
     7021 gggcacggcc ggaggttaga gccacgtcgg cgcccgacgc tcgtagcctc acgggatcgt
     7081 gacctccagc cccaaccccg tccgcgtcgt ggcgtcgggc agcgtcgcgc tcaccaccac
     7141 cgtcgtgccc gccttcctga ccggcgcgct ctccgggcgc atcggtgacg acctcggctt
     7201 cggcgacggg ggagcgggtg ctgcggtcgc cgccttcttc gtcggcgccg gggtcttcgc
     7261 cgtgccgatg gggcgggtga ccgagcgcat cggcccgacc cgggcgctgc gcgccggcgc
     7321 gctcgtcagc gcggccgccg ccctcgccat cgcggcgttc gcatcgagcc tgtgggagct
     7381 cgtcgcgctc ctcgtcgtgg ccggctccgc ggtcggtctc gtggacaccg gcgccgcccg
     7441 cgccttcggg gactcggtcc cgtcgacccg ccacggcatc gccttcgggg ccaaggaagc
     7501 gagcgtgcct gccgcgtcgc tggcggcggg actgtcgatc ccggtgctgg ccgagcagtt
     7561 cggctggcgt tcggccttcg tcgccggcgc gctgctcgcc ccgctcgtct gggtgctcgt
     7621 gccccgcatg gccgccgacc gacccccggc gcgcgacggc agggctggcg acagccgacg
     7681 aggtccgctc gtgctcttcg ccgccggcgt cgccctcggg gtcggtgccg ccaccgcggc
     7741 gtcgaccctc ctcgtaccgg cgatcgagga ccgcggctgg gacgaggccg acgccgggct
     7801 cctgctcgcg gtcgccagca gcgcgagcat cgcggtgcgt ctcgccgccg gcagccgcgg
     7861 cgaccgccgt ccgctcagca cctgggcgat ggtggccacg ctcctcggcg tcggggcggt
     7921 cggtgcggtc ctgctcgccg cgacctcggc cgggccggcc ggcgtgatcg gcgccatcgc
     7981 ggtcatcggc gggggttggg gctggaccgg cttggcgttc cagaccgcgg tccgggtcgg
     8041 ccgcgaccgc ccggccgccg cggccggcgt cgtgctcacc ggcctgtccg tcggaggcgc
     8101 agcgggaccg gccgcgttcg gcgccatcgc gtcggcgctg tcgtactcgg cggcgtgggc
     8161 gaccgccgcc gtggcgctgg tcgccggcgg cgccgcgatc ctggcgtcca agcactggct
     8221 cgagtccggc agcgcgcagg agcggacgac cgccgcgcga tgatgccgcg cgatgatgcg
     8281 ggccatggct ctggacccgc aggcgaaggc actcctcgac gcgctggcgc agatggaggg
     8341 gccgcccatc ctcgagtcgg agccggccga ggtgcgcgag gcgatggctg cgctcggcgc
     8401 catgtccgac gcatccgccg aggaggtccg cgaggtcgtc gagcagacga tcccgggtcc
     8461 cggcggcgac ctgcgggtgc ggatctacac gccggtgctg gccaccgagg gcgccccgca
     8521 ggcgtgcctg gtctggtacc acggcggcgg ctgggtcatc ggcgacctcg acaccaccga
     8581 cggggtgtgc cgggcgctgt gcggacgctc cgccgccacc gtcatctccg tcgactaccg
     8641 gctcgccccg gagcacccgt accccgccgc cctcgacgac gccgaagccg ccttcgactg
     8701 ggtgcgcgac cacgccgacg acctctacat cgcgccggac cgcatcgcgg tcggcggcga
     8761 ctcggccggc ggcaacctgg ccgcgctcgt cgcccagtcc cgtcggggcc aggtggcgtt
     8821 ccagctgctg gtctacccgg cgaccgacct gaccaactcg cacccgtcgc acgaggagaa
     8881 cgccgagggc tacctgctga cccgggacca catgatctgg ttcacgcagc actacctcgg
     8941 cgaccacgac cgcaccgacc cgcgggtgtc gccgctgcac gccgacgacc tgtccggcgt
     9001 ggcgccggcg ttcgtgctga ccgccgagtt cgatcccctg cgggacgagg gtgaggccta
     9061 cgcggcccgg ctggaggacg ccggcgtcga ggtcaccagc agtcgctacg acggcatgat
     9121 ccacgccttc ttccagctga gcggcgccat cgaccgggcg aacgacgccc tcgacgaggc
     9181 ggccgctgcg ctcaggaggg cgctgacgtg aagcaccggc tggcgaccct cgccgtcgcg
     9241 ctggtcctcg tcctcggggc gtgcgagtcc gaccaggatc cgggcatcga ccccggcgtc
     9301 acggacggcc cggccgagac gtcggacacg ctcggcacct gcccggcggg cggtcccgac
     9361 gacacgacgc ccccggccgg ctgcctcggt cccgacggct ccgtcctgcg cccctgaccg
     9421 tcgaccgact cctcagcgga acgcgtccac caccgcggcc cgccactcga tgagagccgg
     9481 gtagcactcg gcggtggact tcccgagccg cacgacgacg aggtccagac cggggcacac
     9541 catgatcgac tgcccttcgt agccgttggc ccagaaggtc ccgacgtcgt cgcccaccac
     9601 ccaccactgg gcgccgtaga gccggccgtc caccgggtcg accgagcggg gccggcggcc
     9661 gtggtcgacc cagccctccg gcagcagccg ggtgccgtcc cacacgccgt cgcgcaggta
     9721 gaggtacccg aagcgggcca tgtccctcgc ggtcgcccac acgtacgacg acgccaccca
     9781 cgtgccggcg tcgtcgaagc gcgggtcggc ggtgcgcatg ccgggcgggt cgaacagccg
     9841 ctcgtggagg aagcgccggt acggctcctc gccgccgagg gcgcgggcca cgacgcccga
     9901 gatgatgttg ctggtgcccg acgagtagtt gaagaccgtg cccggctcgt gggccagggg
     9961 ccgtgacgcg gcgaagccgg ccacgtccga cgcaccggac ccgaagagca tctcgatgac
    10021 gtgggagacc gcgccggcct ggtcgtcggg cgcgtagtcc tcggcgaagt ccaggccgtc
    10081 gcgcatggcg agcagctgct cgagggtgat cgccgcccgc gggtcgcctt cgtcccacgc
    10141 cgggaccggt gccgcgtcgt cgaggcgcag ccggccctcg tcgacgagca tcccgacgac
    10201 ggcgtggagc atggacttgg ccatcgacca cgacagcagc ggggtcgacg cgtcgacggg
    10261 gtcgccggtg acctcgcccc aggcgtcggt gcggtcgccg tagcgctcgg ccaccagccg
    10321 cccgcggtgg accacgacga cggcgcgggt gcggccgtgc ccgctgtcgg ggtccatcac
    10381 cacgtccagg agcggcgcga gggcacggtc ggtgggctcc tcgccggtgg gccactcgtg
    10441 ggtgggccac ggcacgccgt cgggctggcc cggcagcggg acgagcgggc gggcggcgac
    10501 gtcgtcaggg agggtcaccc cggaagtgtg gccgccggcg ggcggccggg tggcctcagc
    10561 ggccgtcgcg gatgtagccg accagctgct gggtctcggt ggccagctcg tcgatgcggg
    10621 ccttgaccac gtcgccgatg ctgatgatgc cgcagagctc gccgtcggcg accacgggca
    10681 ggtgccggaa gcgaccggtg gtcatcgtgg tcatgagctc ctcgacgccg gagcggccgg
    10741 tgcaggtgga cacgtcggtg gccatgaggt ctcgcacgcg gcgctgcagc acctcggcgc
    10801 tctcgccgag gccacggacc acatcgcgct cggagacgat gccctccacc gtcttgccgt
    10861 cgctggacac gacgagcgcg cccacgttgt gctcggcgag cgcggccacg gcctctgcga
    10921 ccgtggcgtc cggcgccacc gtcgcgacgg ctcggccctt ggcttccagg atctggtcga
    10981 ccagcactcc acacctcccc ttggtgtctt cgtctgcagt ttcccacacg ccggtcacgg
    11041 cgtgacggcg gatcagcgtc cgttgcgtga cacgtgctgg tggtcggcgc ccgacgccca
    11101 ccgaccgccc cacccccagc cgagccggtc gaccgcacgg atcaccgcgc tgccctcgac
    11161 cagcatcccg ggccggacgt cgctgcggtc gacgtaggcg ccgccggacg gcggctcgac
    11221 gcggtcgccg cgcacgtagg gattgcgcac cgggttcagg tcgacggcgc ggccgtacgc
    11281 gtgctgcgac cacgtggtgg tgcctgccac cacccggcag ttgaacgccg aggtgttgtc
    11341 cgcggccatc gacgcgtcgt cgtcaccgcc gaagtcgtcg acgggacggg cctgggtgat
    11401 cgggaaccgg ctgccgtacg ccgtccgcac gagcaccgcc acgtcgtggg ccgcgtcgcg
    11461 gtgcacgatg agcacgccgc gcccgatgct gccatcggtc cggaagtggg ggaacgtcac
    11521 ggcgcgcagg tcgccgggtc cgacggggca ccccgggcgc caggaggccg agagcgggta
    11581 ccgggtgccg gcgccgaccc cctcgacccg cacgtccggc agccggacgc cggtggcccg
    11641 gccgtggagc tcgtcgctgg ccgccaggtg cgaggcgagg gcgacgtcgc cgagggcggg
    11701 gaggcgctcg acccagaacg cgcgcccgcc cggatcgggg gcgcggccga ggatctcccc
    11761 gtagagggcg tcgacccgct ggcggcggga ctcgagcgag ccgtagaacg acccggccac
    11821 cgccatgcgg ctcatcccgg cccacatgtg gcccaaccag tgctggcgtc cgccggcgtc
    11881 gggctcccgc tccagcaggc ggcggtagag cgagtcgacg aaggcgtcgg cccgcccgcc
    11941 gacggcgccg tagtactcgt cggagccata gaagccggca gcgaccgact cgagcgtgcg
    12001 cccgcgacgc agctggtcga gccagtacgc ctggccgccg ggatcggggg accggccgag
    12061 gatggtgcgg tacaggtcgt cgatccgcac cccggcccac tcgggggaca gggcgagccg
    12121 cccggtcagg gctgcgggac cgtcgacgtg catcaggtgg gccagctcgg tcgcctccgc
    12181 cggggtggcg gcccgtccga ggaacagctg gtggacgcgc tgcacgaacg cgtgcgtcgg
    12241 cgccggcccg agcgatcccg ggtgggtggg cacctcctgc gcgccagccg ggccggccca
    12301 ccaggcgcag cccagcacga cggcgacgac gaaggccccc agcccccgcc gcgcccggga
    12361 ccggggtcgt gggaacggca cgtcgtcagc ctcgcgcacc cgggccaccg cgtgcggcgg
    12421 cgtggtcctg tggcagcctg cgacggtgag caagcccctg gcgcttcggt tcgtgacctc
    12481 cgccgaccgc ctcgaccgcc tcccgccgac ggcggccgag gtcaccgtcg tgggccggtc
    12541 gaacgtgggc aagtcctcgt tgctgaacgc gctcgccaac cgcacgaacc tggccaagac
    12601 gtcgaagacc ccgggccgca cccagctgct gaactgcttc gagctcgagc cccacgactc
    12661 ggtggagggc accttcgtcg actgccccgg ctacggctac gccaagaccc ccaagtcggt
    12721 gcggcagggc ttccagccga tgatcgaggg ctacctgctc ggccgcgagg agctgcgcat
    12781 ggtgggcgtg ctcgtcgacg gggagatcgg accgacgaag ctcgacgtct tgctgctgga
    12841 gtggctgcgc caccacgacg tgccccacac cgtcatcgcc accaagcacg acaaggtgaa
    12901 gtccaaggcg cgggagaagc gcaagcgcga cctcgccgag gggtgcggcg tggagaaggg
    12961 cgacgtgctc tgggtgtcag cgcacaacgg cgtgaacatc gacgtcctcc gggaccggat
    13021 ccgggggtgg ttggcgtcgt gaccagcacc accgagcggg gggaggaggc cggcgcccgg
    13081 ttcttcccgg gttggatcgt cgtcggcgcc gccttcgtcc tcctcatggc gaactcgggg
    13141 ttcatcttct acgggcaggc cgtgtacctc gacgccctca ccaccgagcg cggcttctcg
    13201 accggcgcgg cgtcgctcgg ccagtcgctc gtgttcgtgg tcggcgggtt ggtcgggcca
    13261 cgcgtcggca agctcatcac cgaccgcgac gtgcgggtcg tggtcctggg tggcctggtc
    13321 acgtcatggg tcgccgtggt gctcctcggc cgggtcgagg agctgtggca gctgtatgcc
    13381 gtgaacgtcc tgctgggcgt gggcttcagc gccgccggcc tggtgccggc gaccacggtc
    13441 gtgacgcggt ggttcgagac caggcgctcg gtcgccctgg ccatcgcgtc gactggcttg
    13501 tccgtcggtg gcctcacctt cacccagctc gtcgcctggt ggatccggcg tgacgggctc
    13561 gagctggcca cgccctggct ggtcgtcgtc tgggccgcgt gcaccctgcc ggtcgtcccc
    13621 ctgctgtggc cgacgccgga gcaccgcggg gtgctggcct ccggggcggt ggccggctcc
    13681 ttcgccatgg gcgtcgccta cgacgtcgcg gtgcgctccc ggttcttcac catggtcacc
    13741 gccggctacg tgctcgtgct cgggatgcag gtcggtggca tcacccacct cgtcaagctc
    13801 ggcaacgagc gggccgggga cggcaccggc ggtcgcctca tcgccgggct caccatcgcc
    13861 agcgtggtgg cgcgcctgat cggcggcgtg gtcgccgaca agcagccgct cgtgcgcctg
    13921 gccgtcgggt tcgccgcgac ccaggccctc gcgctggcgt tgctgtccca ggtcgagtcc
    13981 agcctgctcc tcgtgctgtg cgcagtgctc ttcggagcca ccatcggcaa catcctcatg
    14041 ctgcagccgc tcctgttcgc cgacgccatg ggggcgcgcg actacgcccg gatcttcgcc
    14101 ctgaaccagc tcatcgtggt ggccggggtc gcgctcggcc ccttccttct cggcgccctc
    14161 cacgacgccg ccagctaccg agcgtcctac atcgtcgccg ctgtcctgtg cggcgtgggc
    14221 accgtcctgc tctcacgggc cggaagcacg gcggcggccc gcgcggagct cgactccgag
    14281 cgcccggcga cgacctgatc gcgatggcgc agcggtagtg tctggcgcga tggacgacga
    14341 gctccgcacc cacctcgacc agctcgaggc caagatcgcc gaggcccgtg ccgacggcaa
    14401 catcgacgag gccgagcgcg aagagctgcg cgtgctcgtc gagcgcgtcc gcagcgatct
    14461 gggcagcggc gatcccgacc gggacggcct cgccgagcag ctcgaggagt ccgccgtgcg
    14521 gttcgaatcc gaccacccca cggtggccgc ggtcatccgc agcgcggtgc acaccctcac
    14581 cggctacggc atgtagctcc ggcgctggtc ccgccgccgg gcgggtctac cttgtgcccg
    14641 agggagcttg gggacgaggg aggggcggtg gacgggtcca ccacggatgg gatcgcgtca
    14701 gcgatccggt cggcgacggg gtccatcgtc gtcgtcggag atgcacacca ccgagccgtc
    14761 gccgcgacgg cgccggtcgg cgacgcgcac ctcgtcgagg tccgcccggc gcccgagccc
    14821 acgccgtgga gcgggctcgc cgcgatcctg gacgcctgcc gcagcgagtg gccccggctc
    14881 cccgtgccgg cggcgctcga ggcggccgcc accggccgtc cggtcgggga ccacctcggg
    14941 ctggccggcg acctgggcag cctgctcgcc cgggtggggg aggaggggcc gtggaccctc
    15001 ctggtccacg atgcgcaccg gttcgatctg agcagcgcgg ctgcgctgtc gcgcgccctg
    15061 gcccaggagc cggccggact gcgagccgtg gtcacccagg accgctccgg tcggggcgtc
    15121 gaggggatcc cggtgcacgt ggcgagcggt ggggccgcgc ccatccagga cggcccgccg
    15181 ctcgcggggc cggctgggat cgagggcgcc gagggtcggg ccagcgcggc gctccgcctc
    15241 ggcgcgccgc tcgatgcggc ggcgtgggcg gaagccgcgg gtgactgggc gacggccgca
    15301 gcacggtggc tggagggagg ccggccggac cggtgccgac gcgcgctcgg tctcgccgaa
    15361 cccgatcctc acgtggcgga ggtgaccgcc cggctcgccc accgcaccgg gagcgagcgc
    15421 gagcgccgca tcgccgccca gcgcgccatc ccgatcctcg atgcgcacga ccccctcgcg
    15481 tccgtgcgac tccgcctgct cgaggcgagc gcccgggtct ggtccgacgc ggtcggggcg
    15541 tcggcgaccc tggccgtcgc cgccgacctc ctcgccaccg gcggctcgag cccggccgct
    15601 cggacggtcc gggacctgca tgcgctcgtc gtgagcaccg cggcgctcgg gcgcgacggt
    15661 gaccccgagc cgttgcggtg cagcgtcgag cgcccgctcg cccggctcgg cgccgggggc
    15721 atcgatcccg acgccatcca cctcctcacc gccgcagccc gggtgctggc gtggcaggga
    15781 cacctggacg acgcccggtc gctcctggac cgggtcgtcg gcgtgctcga cgcccgccgc
    15841 cgcttcctgc tgctggccga accgctggcg gcgtccgcct ggcttgctcg ccgccgcggt
    15901 cgactcgagc tggcgctgac ccacggggcg cgggcgctgg acatcgcgcg ggcgtgcggc
    15961 tggaccagcg acgagcggat cgcgacggtg gaggtcgccc acatcgaggc gatgcgcggc
    16021 cgtctcgacg actgccgccg ccacgtcgcc acgctcgtcc caccggggac ggttccccgg
    16081 ggggcggccc agatcggggc ggtgtcggcc ctggccgtcg gcgagctgct ggccgatgca
    16141 ccggagcggg cggtggagct gctcgagccg gtgcaggagc ggttcggtgc gtcgatctct
    16201 ccggccgaga tcgcctggcg gcacaacctc gtcgaggcct acgtccgcac ggggcgcgac
    16261 gcggacgcgg cgagggtcct ggacgacctc gtcggatggg tggcgcgcac cgactcgaca
    16321 cgggagcagg gccaggtcgc gtggtgccgg ggcatgctcg ctccgcccgg cgagcacgac
    16381 gcggacttca ccgaggcccg gcggctgctc gatgcgtacc cgaccctgcg ctggcgatcc
    16441 gacctgcacc acctccggcg gctgctcgac gagcgacgcc acgacaccgc tgccgatgtg
    16501 gcggggcgac tggtgtccga ggcgaggagc gcgggcctgc tccacgccgt tgagcgggtg
    16561 caccggctcc agcgggagca cggcatcgac gtgcccgaac ccgccccgtc gacgtcggtg
    16621 ctctccgtgg aggacctgcg ggtggcgctc gccctggcgg aggggaccga ggtcgaggag
    16681 gtcgcgacgc tgctgcagct gaccccacga cgcgtcgagg tggtgcgcga ccgcgtcatg
    16741 ggtctgctcg gcgtcgacgc gcccgagcag ctggccggct tcctccccca ggaccgagcg
    16801 acaccggtgc ccgtcgccgc ggtcgtgtgc gtcctcggcc ccaccgtcgt gcaccgaggg
    16861 gcgcagcagc tggtcccccc ggcgggccgt cccgaagcgg tgctcgccct ggtcgccacc
    16921 gaacgagacg tcccggtcga gcgggtgctc gacgtcctgt ggcccgacgt cgaccctggt
    16981 cggtcacggt cccggctccg caacgtcctg tcccggctgc gggccgccgc cggcgcgctg
    17041 gtcgagcgcg acggcgaccg cctcgtgctg tccgccggcg tggaggtgga cgcccaccgc
    17101 ttcgatcagc tggccgagct cgcgctgcgg gcaccgccgg acgaggtcct cgcccgcacc
    17161 gacgcggccc tcgccgcctg gcacggcgaa ccgctgccgg cgtggcccta cgaggactgg
    17221 gcgctgcgcg agcggcaccg gctggtgcag cgctgcgtcg cgctgcacgt ccggcgggcc
    17281 gagatccacg ccgcgagcgg ggcggtgggc gccgccctgg acgagctcga gctggccgtc
    17341 gggttgcagc ccgacgcgcg tgacctgtgg gagcgcgccg tgcacctggc cgaggcggac
    17401 gagcgcatcg gccgggcgcg ggcgctgcgg cgccgcgccg ccgaccacga cgtcgatctg
    17461 gtctgaccgg ggatgctcag gggctcggcg tgtcggctcg ctggtccatg ccctcggcca
    17521 ccggctcggt cgcggacgac acggtgtcga acagcgtcag gccgccgagg accagcagtc
    17581 ccaccaggac ggcttggagc agcagggagc gggttcgttc ggcggcgcag tgctcgtaca
    17641 cgtccggcac tcctcggagt cgggtcggga acgccgtcag catgtgatga cggcgtgtca
    17701 cggccgtgtc acgagcgatc accgcgagcg cgcatcgcgt cccgtcgccg tcacgccaac
    17761 ggcgatccag gcggtatgcc ggaggtgtcc ggtgccgccc tgggtggtca ggcgcgcacg
    17821 acgtagcggt tgcggccctg cgacttcgct tcgaggagcg cacggtcgct ctcgcgcagc
    17881 aggtcgtcga gcggggtggc gaaggtgccg aacgagacgc cgacgctgag cccgacgcgg
    17941 gcggtgtgct gctcgaggcc gatcgggtcc cggacgtggt cgatcaggcg ccgggccagg
    18001 gcctcggcgg cggcaggttc ggcgcagacg acgacgaact cgtcgccacc ccagcggcag
    18061 accaggtcgc cgtcgcgcac cgaccggcgc agtcgctcgg ccaccacctg gagcgtgcgg
    18121 tcgcccatgg cgtggccgag gtggtcgttg acgtacttga actcgtcgac gtcgcagaag
    18181 agcagcgcga gcccctcgtc ggcgcgatcg accgcctggg caccgagcca ctcgtcgaga
    18241 ccgctgcggt tgggtagctc cgtgagctgg tcggtgacgg ccgatcgccg ccacgctcgc
    18301 cggttccggt cggactcgat ggccagtgcg accagggtcg ccacccgctc gaccgtggtg
    18361 ctgtgcggca ggggcggtgc gtccatcggg cgcagcagca ccagcgtcgc ggagggtcgt
    18421 tggtcgccgc gcaccgtgat ggggatcgac cagcacgagg cgagcccggc ctcgtcggcc
    18481 aggtccgggt tgctgacggg tccggtgccg tcgccgtgcc agtcgatcac cggcatctcg
    18541 gcgatgcgcg ccgcgaggtc gcggtgcgtc gacgcggtgc ccggggcggt caccaccacg
    18601 tcgaagcggt cgctgtccca gccggcggcg acgaacacca ccgaacccgg catgtcgtgc
    18661 tccacgatcg cggcgagctt ggtcagcacg tcgtcgatcg gctctccggc cgcgagcgcg
    18721 tcgaccgcgg cgtagagcag cacggtgccg cgccaccggc ggacctgcac gacgatgccc
    18781 tcgatgccgt gggtggtggc ggtggccgcc gagacctcgc acgtgatccg cgacccgtcg
    18841 gcgtgcacca tgtccaggag catcggcggg ccgacccacg ctcgacggag ctggtcgtcg
    18901 gacgtgaact cggcgtactc ggcgagcacc gcctcgtgcg aggacgggtc gatgaggtcg
    18961 aggaagtgga gcccgatgac atcgtctgcg gtgcggccga tgagcatctc caccttctcg
    19021 ctgcaccacc gcacgtgccc cgcggggtcg agcaccacga aggggttcac cgacgtctgc
    19081 acgatggccc ggaacatggc gtcgctgccc ggctccgcgc gcgccgggga cgaggtcacc
    19141 tggtccagcg tgtgctccgt gctcatgagg tgccctaccc gcgcgccgac tgcaccgcgc
    19201 aacctaggcg gca
//
LOCUS       DATISU010000358         1593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_772167, whole genome shotgun sequence.
ACCESSION   DATISU010000358 DATISU010000000
VERSION     DATISU010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772167"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1593
                     /locus_tag="VLR27_14290"
     CDS             <1..>1593
                     /locus_tag="VLR27_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04677.1"
                     /translation="LDGDEAIGEPLLDDDGVAWLLAEHAAGHRAWRVGHDLEPTTRDV
                     PPTTTSLAVVDGEVVAVTRTGVVAVGGGDDRSAPSGATVPTVAVGTGDAWAHAEGAQV
                     TVLDGGRSSEVTLEDPVEQLVVWSGAVLAVTAGGVHRVEGGDTTPVGEFDDAPRVHTD
                     GGLLWLTSRNRVTAVWDDHTEVTFELAAIDLDLCVGDCEADDVRRFLDQQEEEEEERD
                     EPTERTTTTLDRQQSPTTTRQLRPPSVEPSIPTTTTTTTTRPPSTTTTTTRDRSGGEE
                     REERPTTTRPRPTATTQPTTTTRPTTTTAAPAPPPPPPPRPTWLPPLLPGPRDPGPGD
                     PGPSDPGPGDPGPGPGNGGGPGRPPGPPPTEATTTTAPAAQSTTTTTEPTTTTTEPPP
                     GSVSLVLGVDRDGGDATATVGVQGRAIDCGALVSRQTTGTVSWSGAASGSTQVRLAWG
                     GPGDRSTDIEEVTIANVRNGDLTVSFSACGVQTSQTAGDPDAPPRVSAVTVSPAQPEV
                     GQSFTASVSVEEANGWTVSGAEW"
BASE COUNT          226 a          640 c          572 g          155 t
ORIGIN      
        1 ctcgatggcg acgaggcgat cggcgagccc ctcctcgacg acgacggcgt cgcatggctg
       61 ctggccgagc acgccgccgg ccaccgggcc tggcgggtgg gccacgacct cgagcccacc
      121 accagggacg tgcccccgac caccacgtca ctcgccgtgg tcgacggcga ggtcgtggcc
      181 gtcacccgga ccggcgtcgt ggcggtgggc ggcggcgacg accgctcggc tcccagcggc
      241 gccacggtcc cgaccgtggc cgtcggcacc ggcgatgcct gggcccacgc cgagggtgcg
      301 caggtcaccg tgctcgacgg cggccggtcc agcgaggtga cgctcgagga tccggtggag
      361 cagctggtcg tgtggtccgg tgccgtcctc gccgtcacgg ccggcggcgt acaccgcgtg
      421 gagggcggcg acacgacgcc ggtgggcgag ttcgacgacg ccccccgggt gcacaccgat
      481 ggcggcctcc tctggttgac ctcgcgcaac cgggtcaccg cggtgtggga cgaccacacg
      541 gaggtcacct tcgagctggc cgccatcgac ctcgacctct gcgtcggcga ctgcgaggcc
      601 gacgacgtcc gtcgcttcct cgaccagcag gaggaggagg aagaggagcg cgacgagccc
      661 accgagcgca ccaccacgac gctcgaccgc cagcagtcac cgaccaccac ccgccagctg
      721 cgacccccgt cggtggagcc ctcgatcccg acgacgacca ccacgacgac gacgcggcca
      781 ccgtcgacca ccaccacgac gacgcgggac cgcagcggcg gtgaggagcg cgaagagcgc
      841 ccgacgacga cccggcctcg gccgaccgcc acgacccagc cgaccacgac cacccggccg
      901 acgacgacca cggcggcgcc cgcgccgccc cctcccccgc cgccccggcc gacgtggctc
      961 ccgccgctgc tgcccggacc ccgcgacccc ggaccgggcg acccgggtcc gagcgatccc
     1021 gggcccggcg accccggtcc gggccccggg aacgggggtg gccccggccg ccctccgggg
     1081 cccccgccga ccgaggccac gacgacgacc gccccggcgg cccagtcgac cacgaccacc
     1141 acggagccga cgacgaccac caccgagccc ccgcccggct cggtcagcct cgtgctcggc
     1201 gtcgaccgcg acggtggcga cgccaccgcg acggtgggcg tccaggggcg cgccatcgac
     1261 tgcggcgcgc tcgtcagccg ccagaccacg ggcaccgtct cgtggtccgg cgccgcctcc
     1321 ggcagcaccc aggtcaggtt ggcgtggggc gggccaggtg accggtcgac cgacatcgag
     1381 gaggtcacga tcgccaacgt ccgcaacggc gatctcacgg tgtcgttctc ggcgtgcggc
     1441 gtgcagacct cgcagacggc aggcgacccc gacgcgccgc cgagggtgtc ggccgtgacc
     1501 gtgagcccgg ctcagcccga ggttggccag tcgttcaccg cctcggtgtc ggtggaggag
     1561 gcgaacggct ggacggtcag cggcgccgag tgg
//
LOCUS       DATISU010000359         1958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_214390, whole genome shotgun sequence.
ACCESSION   DATISU010000359 DATISU010000000
VERSION     DATISU010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214390"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /gene="grpE"
                     /locus_tag="VLR27_14295"
     CDS             complement(<1..452)
                     /gene="grpE"
                     /locus_tag="VLR27_14295"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HSP04678.1"
                     /translation="MADDDLTPTDDIDDPAHVSEALASDAEAVAESVDAHADADHHLA
                     DEPLEFTPEQLDALVAERDEFLDAYRRTAADFDNYRKQAQKRITDEVARSQGGFVERM
                     LPVLDAIEAAQTHAGPGESGVEAVSKALFGFLEKEGLERVAPTGEPFDP"
     gene            complement(458..>1958)
                     /gene="dnaK"
                     /locus_tag="VLR27_14300"
     CDS             complement(458..>1958)
                     /gene="dnaK"
                     /locus_tag="VLR27_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HSP04679.1"
                     /translation="TVTQAVITVPAYFDDAQRTATKEAGQIAGLEVLRIINEPTAAAL
                     AYGLDKEGDADQTVLVFDLGGGTFDVSVLEIGDGVFEVKSTHGDTNLGGDDWDQKIID
                     WLVAQFKSAHGVDLSKDNMAEQRLKEAAEKAKIELSQVQQTQINLPFITATQEGPLHL
                     DEQLTRAKFQELTQDLLDRCKSPFEQAISDAGLSKGDIDHVILVGGSTRMPAVTDLVK
                     EFTGKDPNKSVNPDEVVAIGAAVQAGVLKGDVKDVLLLDVTPLSLGIETKGGVMTRLI
                     ERNTTIPTKRTEVFTTAEDMQPSVEIHVLQGEREMASFNKTLGKFQLVDLPPAPRGVP
                     QIEVTFDIDANGIVHVSAKDRATDKTQSMTITGQSSLAKDDIDRMVKDAEAHAEEDAR
                     RKEEAEARNQGDTLVYQTEKLLKEQGEKLSGDEKQKVEDALASLKTALAGDDVAAIKE
                     ATEALMTASQEFTQKLYESAAQSEGGAAGGTGATSADDDEVVDAEIVDE"
BASE COUNT          233 a          627 c          724 g          374 t
ORIGIN      
        1 gggtcgaagg gctcgccggt gggggcgacc cgttcgaggc cctccttctc gaggaacccg
       61 aacagcgcct tggacaccgc ctcgacaccg gactcgccgg ggccggcgtg ggtctgcgcc
      121 gcctcgatgg cgtcgaggac ggggagcatg cgctccacga agccgccctg gctgcgggcc
      181 acctcgtcgg tgatccgctt ctgggcctgc ttgcggtagt tgtcgaagtc cgccgccgtg
      241 cgccggtacg cgtcgaggaa ctcgtcgcgc tcggccacca aggcgtcgag ctgctcgggg
      301 gtgaactcga gcggctcgtc ggccaggtgg tggtcggcgt cggcgtgggc gtcgaccgac
      361 tcggcgaccg cctccgcgtc cgaggcgagc gcctcggaca cgtgggcggg atcgtcgatg
      421 tcgtcggtcg gggtcaggtc gtcgtcggcc acgtcgatca ctcgtcgacg atctcggcgt
      481 cgacgacctc gtcgtcgtca gccgaggtgg caccggtgcc acctgcggcg ccgccctcgg
      541 actgggccgc cgactcgtag agcttctggg tgaactcctg cgacgccgtc atgagggcct
      601 cggtggcctc cttgatggca gccacgtcgt cgccggcgag cgcggtcttc agcgaggcga
      661 gggcgtcctc gaccttctgc ttctcgtcgc ccgacagctt ctcgccctgc tccttgagca
      721 gcttctcggt ctggtacacg agggtgtcgc cctggttgcg ggcctcggcc tcttccttgc
      781 ggcgggcgtc ctcctcggcg tgggcctcgg cgtccttcac catgcggtcg atgtcgtcct
      841 tggccagcga gctctgaccg gtgatcgtca tcgactgggt cttgtcggtg gcccggtcct
      901 tggccgacac gtggacgatg ccgttggcgt cgatgtcgaa ggtgacctcg atctggggca
      961 cgccacgggg ggcggggggc aggtcgacga gctggaactt gccgagcgtc ttgttgaacg
     1021 acgccatctc gcgctcgccc tgcaggacgt ggatctccac cgacggctgc atgtcctcgg
     1081 cggtggtgaa cacctcggtg cgcttggtgg ggatggtggt gttgcgctcg atgaggcggg
     1141 tcatcacgcc gcccttggtc tcgatgccga gcgacagcgg ggtgacgtcg agcaggagca
     1201 cgtccttgac gtcgcccttg agcacgccgg cctggacggc ggcgccgatg gccacgacct
     1261 cgtcggggtt gaccgacttg ttcgggtcct tgccggtgaa ctccttgacc aggtcggtca
     1321 cggccggcat gcgggtggag ccgcccacga ggatgacgtg gtcgatgtcg cccttggaca
     1381 ggccggcgtc gctgatggcc tgctcgaacg gggacttgca gcggtcgagc aggtcctggg
     1441 tgagctcctg gaacttggcc cgggtgagct gctcgtcgag gtggagcgga ccctcctgcg
     1501 tggcggtgat gaacggcagg ttgatctggg tctgctgcac ctgggacagc tcgatcttgg
     1561 ccttctccgc ggcctccttg aggcgctgct cggccatgtt gtccttgctc aggtcgacgc
     1621 cgtgggcgga cttgaactgg gcgaccagcc aatcgatgat cttctggtcc cagtcgtcgc
     1681 cgccgaggtt ggtgtcgccg tgggtggact tgacctcgaa cacgccgtcg ccgatctcga
     1741 ggaccgacac gtcgaaggtg ccgccgccga ggtcgaagac gagcacggtc tggtcggcgt
     1801 cgccctcctt gtcgagcccg taggccaggg cggccgcggt gggctcgttg atgatgcgca
     1861 gcacctcgag gccggcgatc tggccggctt ccttggtggc ggtgcgctgg gcgtcgtcga
     1921 agtaggccgg cacggtgatg acggcctggg tgacggtg
//
LOCUS       DATISU010000360         7435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_493455, whole genome shotgun sequence.
ACCESSION   DATISU010000360 DATISU010000000
VERSION     DATISU010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7435
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493455"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VLR27_14305"
     CDS             <1..411
                     /locus_tag="VLR27_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HSP04680.1"
                     /translation="YVDGDWFRTGDIGYFDDDGYLYIHDRVKDMIVSGGENVYPAEVE
                     NALMKHPGVADVAVIGVPDEKWGEVGKAMVVKAEGQDPDPDEIISFAREQLAGFKVPK
                     SVDFIDEIPRNPTGKMLKKDLRAPFWEGRERNVN"
     gene            complement(486..821)
                     /locus_tag="VLR27_14310"
     CDS             complement(486..821)
                     /locus_tag="VLR27_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04681.1"
                     /translation="MSNRHRSHKETAPGATHPAPPKEERKAANRKARHRTNELLAHAD
                     LESGEEIVPPRPVHTRLDTERGDASGSSERPRREYRHWKQKFWKRRTNVRAQKAMLAN
                     QELPTPEIG"
     gene            948..2261
                     /gene="serS"
                     /locus_tag="VLR27_14315"
     CDS             948..2261
                     /gene="serS"
                     /locus_tag="VLR27_14315"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HSP04682.1"
                     /translation="MLDLRRIRSEPDAIRAGLSRRGADLSLLDRVLELDERARAVQAE
                     VDELRNQVKTVSKEIGELFKAGEREEAEARKAESKRLGDREKELAAAADGLQTDIRDA
                     LLRIPNLPSDDAPDGASEADNVVLRTHGPGPETFAEHQRVPHWDIGTELGIIDGERAV
                     KISGSMFTMYRGWGARLLRAMIGHSLDASADPANGAVWEEIRPPTLVKTDTMIGTGHL
                     PKFEDEAYHVERDDLWTIPTAEVPLTSMAADEVLDWDTLPVRFTAYTSCFRREAGAAG
                     RDTRGLLRSHEFDKVELMGYADGAEQAIALQEEILARSEQILQDFGLSYRILDLCAGD
                     LGGSAARTWDIEAYAPGVGEWLEVSSVSWFADYQARRANVRWKRAGEKGTQVCHTVNG
                     SAMGWPRTVAALLETHRQPDGSVAIPEVLQPRLGGMSHIPAPGGG"
     gene            2273..4228
                     /locus_tag="VLR27_14320"
     CDS             2273..4228
                     /locus_tag="VLR27_14320"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSP04683.1"
                     /translation="MHNLLGVSMPFEDPALVLVTVFAVVLFAPVLAGLARIPPIIGLI
                     AGGILVGPNGLELVERVGTIELLGGAGLLFLMFQAGIELDLDDFAAHRNGAVIFGLAT
                     FLLPMAIGTVGHLLLGFDAMAAVLLASCWASHTLLSYPTFQRLGVVTNRSVSTAVGAT
                     IITDIAALMVLVVVIAADGGDLDVAFFAGLLPALAAAFAFILLVLPRLARWFFKRAGR
                     DRISRFLFVCLALYGSAAIAEVAGTEAIIGAFLAGLALNRLIPHGGLLMERIDFFGGT
                     FLVPIFLISVGMLVDLGALADAETLGWAAGFCAVVVGAKLAAAALAGRVLHFGSAEVG
                     SMFSLSVAQAAATLAAVFVGLEAGIIDGRAVNAVVVVILVTSLLSSLGAARYGAQLPV
                     PPMKELTLGQRILVGLPEQGDPSAMVDLASRLAAPESGSVLPARILDLESQPADVQAK
                     RAAIVETLEPRILGSGAEVTPIIRLDLTPNAGLLHATIEQQATCLLLGWRGYTARRDA
                     ILGEQLDALLALSPVPVMVHREGAESGIRRVVLAVDPGDLSPSGRRTLDLTLLVSARL
                     ATSYKVDWTVLTPADAPIIAEVESLDLHPEITVDARPLHVAVADVVGPSDLLVLGIPR
                     ALDGLGAGVARAARAVPGCRMLAVAAR"
     gene            4249..6177
                     /locus_tag="VLR27_14325"
     CDS             4249..6177
                     /locus_tag="VLR27_14325"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HSP04684.1"
                     /translation="MTELGALLWTPSSERVAATRMARFAERLGCEGYEALHELSVSDP
                     DRFWPAVWDECGLVGDPGGTVLDPGRSMPEARLFPDASLSIVEHVLGHTGSEPAIIAE
                     SETGSRSMSWDHLAVEVGAMAAALAEVGVGEGDRVAAWLPNGIEAVVAMLGAAAIGAV
                     FSSTSPDFGAAGVLDRFGQIEPVVLFAVDGYDYGGTHFDRRDVRAEIEAGLPALRRTV
                     VLGHDWNAFLEPHRGTPAPLTRRSFHHPWYVLYSSGTTGTPKCIVHATGRVLLQHAKE
                     HQLHTDLSAGDRLLYVTTCGWMMWNWLVSALASGVTIVTVDGNVAHPGPSRLWELVDR
                     HGVDVLGVGAKYLDALAHQGYAPGEHHDLTSLRTLASTGSPLSPERFAWVHEHVKADV
                     HLASISGGTDLCGCFVLGDPTSPVHAGEIQRPGLGMAVDVWSEDGEPLGAGERGELVC
                     TRPFPSQPLGFWGDDDGSRYRAAYYDRFPDARDGYGVWHHGDFASWTEHGGVVIHGRS
                     DTTLNPGGIRIGTAEIYRAVEGLSGIAESLVFGEEVTDDDGGTDVRIVLLVVLDDGVA
                     LDDELRKRIADTVRSSCSPRHVPRRIVAVDDLPRTRSGKLVELAVSDAVNGREVRNTE
                     ALLDPEVLWAVRDAVTGD"
     gene            complement(6256..6669)
                     /locus_tag="VLR27_14330"
     CDS             complement(6256..6669)
                     /locus_tag="VLR27_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04685.1"
                     /translation="MNVAAQMLEARSDERGVDHAALALAIDAMHDVVSATTSCADACL
                     SEPDVADKTECVRACLDAADVATALIKVLSRTGPTVQGTQALLSATAKMLAETSAVAA
                     SHGDHDKHCRITAETTSRGQQALASLEAAVAAAES"
     gene            6748..7386
                     /gene="pdxH"
                     /locus_tag="VLR27_14335"
     CDS             6748..7386
                     /gene="pdxH"
                     /locus_tag="VLR27_14335"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HSP04686.1"
                     /translation="MDPEVLARRRREYEAQGFDVADADPDPLVQFHRWFDAVDEPMDE
                     PHAMVLATVQPDGTPSVRTVLLRGVDERGLGFFTNYESAKADAIEAQPVVECLFLWLP
                     VHRQVRVRGRAEKVSVEESDEYFASRPRGSQLGAWASPQSQVVSGREELERYLAAAEE
                     RFTHEVPRPPHWGGYRVVPDGWEFWQGRPGRLHDRVRYRREGGAWVRERLAP"
BASE COUNT          952 a         2701 c         2682 g         1100 t
ORIGIN      
        1 tacgtcgacg gggactggtt ccgcaccggc gacatcggct acttcgacga cgacggctac
       61 ctctacatcc acgaccgggt gaaggacatg atcgtctccg gcggcgagaa cgtgtacccg
      121 gccgaggtcg agaacgccct gatgaagcac ccgggcgtcg ccgacgtcgc cgtgatcggc
      181 gtccccgatg agaagtgggg cgaggtcggc aaggccatgg tggtcaaggc cgagggccag
      241 gaccccgacc cggacgagat catcagcttc gcccgcgagc agctggccgg gttcaaggtg
      301 cccaagtcgg tggacttcat cgacgagatc ccccgcaacc ccaccggcaa gatgctgaag
      361 aaggacctgc gcgccccgtt ctgggagggc cgcgagcgca acgtcaacta gtccgatcct
      421 ccgctggacg cgcaacggcc cggggcacct gccccgggcc gtgtcgcgct cggcgtcagt
      481 cggagtcagc cgatctccgg ggtcggcagc tcctggttgg cgagcatcgc cttctgggct
      541 cgcacgttgg tgcgccgctt ccagaacttc tgcttccagt gccggtactc ccgtcggggt
      601 cgttcgctgg agccgctggc gtcaccgcgc tcggtgtcga gccgggtgtg caccggtcgg
      661 gggggcacga tctcctcgcc ggactcgagg tcggcgtggg cgagcagctc gttggtgcgg
      721 tggcgggcct tgcggttggc cgccttgcgc tcctccttcg gcggagccgg gtgcgtggca
      781 ccgggggcgg tctccttgtg cgaacggtga cggttggaca tggcactcct cgtcagttcg
      841 gggtcccgtc atccgacggg tccgggacca gaacgtacga aacgaatgtt acggaccgat
      901 gacggaactc ggtttggtga ccttgggccc tccagtaccg tgcctccgtg ctcgacctgc
      961 gccgcatccg gtccgaaccc gacgcgatcc gcgccgggct gtcccggcgc ggggccgacc
     1021 tgtccctgct cgaccgggtg ctcgagctcg acgagcgggc ccgggccgtg caggccgagg
     1081 tggacgagct gcgcaaccag gtcaagaccg tctccaagga gatcggcgag ctgttcaagg
     1141 ccggcgagcg cgaggaggcc gaggcccgca aggccgagtc caagcgcctc ggcgaccggg
     1201 agaaggagct ggcggccgcc gccgacgggc tccagaccga catccgcgac gcgctgctgc
     1261 gcatccccaa cctgccgagc gacgacgccc ccgacggcgc cagcgaggcc gacaacgtcg
     1321 tgctgcgcac ccacgggccc gggccggaga ccttcgccga gcaccagcgg gtgccgcact
     1381 gggacatcgg caccgagctc ggcatcatcg acggcgagcg ggccgtgaag atcagcggct
     1441 ccatgttcac catgtaccgg ggctggggtg cccggctgct gcgggccatg atcggccaca
     1501 gcctcgacgc cagcgccgac ccggccaacg gcgccgtgtg ggaggagatc cgcccgccga
     1561 ccctggtgaa gaccgacacg atgatcggca ccggccacct gcccaagttc gaggacgagg
     1621 cgtaccacgt cgagcgcgac gacctctgga cgatccccac cgccgaggtg cccctcacgt
     1681 ccatggccgc cgacgaggtc ctcgactggg acaccctgcc ggtgcggttc acggcgtaca
     1741 cgtcgtgctt ccgccgtgag gccggcgccg ccgggcgcga cacccggggc ctgctgcgca
     1801 gccacgagtt cgacaaggtc gagctcatgg ggtacgccga cggcgccgag caggccatcg
     1861 ccctgcagga ggagatcctc gcccgcagcg agcagatcct ccaggacttc ggcctcagct
     1921 accgcatcct cgacctgtgc gccggcgacc tcggcggctc ggccgcccgc acgtgggaca
     1981 tcgaggccta cgcgccgggc gtgggcgagt ggctcgaggt gagctcggtc agctggttcg
     2041 ccgactacca ggcccgccgg gccaacgtgc gctggaagag ggcgggcgag aagggcaccc
     2101 aggtgtgcca cacggtgaac ggctcggcca tgggctggcc ccgcaccgtg gccgccctgc
     2161 tcgagaccca ccgccagccc gacgggagcg tcgccatccc cgaggtcctc cagcccagat
     2221 tgggcggaat gtcacacatc cccgcccccg ggggtgggta ggggacgccg ccatgcacaa
     2281 cctgctcggg gtctcgatgc ccttcgagga cccggccctg gtgctggtca ccgtgttcgc
     2341 ggtggtcctg ttcgcaccgg tgctcgcagg gttggcgcgc atccccccga tcatcggact
     2401 gatcgccggc ggcatcctcg tcggtcccaa cggcctggag ctggtcgagc gggtcgggac
     2461 catcgagctg ctcggcggcg ccgggctgct cttcctcatg ttccaggccg ggatcgagct
     2521 ggacctggac gacttcgccg cccaccggaa cggggcagtg atcttcggcc tcgccacgtt
     2581 cctgctgccc atggcgatcg gcaccgtcgg gcacctcctg ctcggcttcg acgcgatggc
     2641 ggcggtgctg ctcgcgtcgt gctgggcgtc gcacacgctg ctgtcgtacc cgacgttcca
     2701 gcgcctcggt gtggtcacga accggtcggt gtccacggcc gtcggggcca cgatcatcac
     2761 cgacatcgcc gcgctgatgg tgctggtcgt ggtcatcgcc gccgacggcg gcgacctgga
     2821 cgtcgccttc ttcgccggcc tcctgccggc gctcgccgct gcgttcgcgt tcatcctcct
     2881 cgtcctgccc cggctcgccc gctggttctt caagcgagcc ggacgagacc gcatcagccg
     2941 gttcctgttc gtgtgcctgg cgctgtacgg ctcggccgcc atcgccgagg tggccggcac
     3001 cgaggcgatc atcggggcct tcctcgccgg cctcgccctc aaccgcctga tcccccacgg
     3061 cgggctgctc atggagcgga tcgacttctt cggcggcacc ttcctcgtgc cgatcttcct
     3121 catctcggtc ggcatgctcg tggacctcgg cgccctggcc gacgccgaga ccctcggctg
     3181 ggcggccggg ttctgcgccg tcgtcgtggg cgccaagctc gccgccgccg cgctggccgg
     3241 ccgggtcctg cacttcggat ctgccgaggt cggatcgatg ttctccctgt cggtggccca
     3301 ggcggccgcg accctggccg cggtgttcgt cggcctcgag gccgggatca tcgacggtcg
     3361 ggcggtcaac gccgtcgtcg tggtcatcct ggtgacgagc ctgctgtcgt cgctgggcgc
     3421 cgctcgctac ggggcccagc tgccggtccc gccgatgaag gagctgacgc tcggccagcg
     3481 gatcctcgtc ggcctccccg agcagggtga cccgtccgcc atggtcgacc tcgcgtcccg
     3541 cctggcggcg cccgagagcg gatcggtcct gccggcgcgc atcctcgacc tcgagtccca
     3601 gcccgccgac gtgcaggcga agcgggcggc gatcgtcgag acgctcgagc cccggatcct
     3661 cggcagtggc gccgaggtga ccccgatcat ccgcctcgac ctcaccccga acgccgggct
     3721 cctgcacgcc accatcgagc agcaggccac ctgcctgctg ctgggctggc gcggctacac
     3781 cgcccgccgc gacgccatcc tcggcgagca gctcgacgcg ctgctggcgc tctcgccggt
     3841 gccggtcatg gtccaccgcg agggcgccga gtcggggatc cgccgggtcg tgctggccgt
     3901 ggaccccggt gacctgagcc cgtcgggtcg gcgcaccctc gacctcaccc tgctcgtgtc
     3961 ggcccgcctc gccacgtcct acaaggtgga ctggaccgtg ctgacgccgg ccgacgcacc
     4021 gatcatcgcc gaggtcgagt cgctggacct ccaccccgag atcacggtcg atgcccgccc
     4081 gctccacgtc gccgtcgccg acgtggtcgg cccgtccgac ctgctggtgc tcggcatccc
     4141 gcgggccctc gacggcctcg gcgccggcgt ggcccgggcc gcccgcgccg tccccggctg
     4201 tcgcatgctc gccgtcgccg cccggtagcc cggggtacgg tcgccggcgt gaccgagctc
     4261 ggggcgctgc tgtggacgcc gtcgtcggaa cgggtggccg ccacccggat ggcgcgcttc
     4321 gccgagcggc tgggctgcga gggctacgag gcgctgcacg agctgtcggt ctcggacccg
     4381 gatcggttct ggccggccgt gtgggacgag tgcggcctgg tcggtgaccc gggtggcacc
     4441 gtgctggacc cggggcggtc catgcccgag gcccggctgt tccccgacgc ctcgctgagc
     4501 atcgtcgagc acgtgctggg ccacaccggg agcgagccgg cgatcatcgc cgagtccgag
     4561 accggcagcc gctcgatgtc ctgggaccac ctcgcggtcg aggtcggggc catggccgcc
     4621 gcgctcgccg aggtcggcgt cggcgagggc gaccgggtgg cggcgtggct gcccaacggc
     4681 atcgaggccg tggtcgccat gctcggggcg gcggcgatcg gtgccgtctt ctcctccacc
     4741 agcccggact tcggtgccgc cggcgtgctc gaccgcttcg gccagatcga gccggtggtc
     4801 ctgttcgctg tggacggcta cgactacggc ggcacccact tcgatcggcg tgacgtccgg
     4861 gccgagatcg aggccgggct gcccgcgctg cggcgcacgg tggtgctcgg gcatgactgg
     4921 aacgcgttcc tcgagcccca ccgcggcacg cccgcgccgc tgacccgtcg cagcttccac
     4981 cacccctggt acgtcctgta ctcgagcggc acgaccggca cgccgaagtg catcgtgcac
     5041 gccaccggcc gggtgctgct ccagcacgcc aaggagcacc agctccacac cgacctgtcc
     5101 gccggcgacc gcctgctcta cgtcacgacg tgcggctgga tgatgtggaa ctggctggtc
     5161 tcggcgctgg cgtccggggt cacgatcgtg accgtcgacg gcaacgtcgc ccaccccggt
     5221 ccgtcgcggc tgtgggagct ggtcgaccgc cacggcgtgg acgtcctcgg cgtcggcgcc
     5281 aagtacctcg acgccctggc ccaccagggg tacgcaccgg gggagcacca cgacctcacg
     5341 tcgctgcgga cgctggcgtc caccggctcg cccctgagcc cggagcgctt cgcctgggtg
     5401 cacgagcacg tgaaggccga cgtgcacctc gcctcgatca gcggcggcac cgacctgtgc
     5461 ggctgcttcg tgctgggcga cccgacctca ccggtgcacg ccggtgagat ccagcgaccc
     5521 ggcctcggca tggccgtcga cgtctggtcc gaggacgggg agccgctcgg cgcgggggag
     5581 cggggtgagc tcgtgtgcac ccggcccttt cccagccagc cgctcggctt ctggggtgac
     5641 gacgacggca gccggtaccg ggccgcgtac tacgaccgct tcccggacgc ccgcgacggc
     5701 tacggcgtgt ggcaccacgg ggacttcgcg agctggaccg agcacggtgg cgtggtcatc
     5761 cacggacgca gcgacaccac cctgaacccc ggcggcatcc gcatcggcac cgccgagatc
     5821 taccgggcgg tcgaaggtct gtccggcatc gccgagtccc tggtgttcgg cgaggaggtc
     5881 accgacgacg acggcggcac cgacgtgcgc atcgtgctgc tggtggtcct cgacgacggg
     5941 gtggcgctgg acgacgagct gcggaagcgc atcgccgaca ccgtccgatc gagctgctcc
     6001 ccccgccacg tgccccgccg catcgtggcg gtcgacgacc tgccccggac ccgctccggg
     6061 aagctggtcg agctggccgt gtccgacgcc gtgaacggcc gcgaggtgcg caacaccgag
     6121 gcgctgctcg acccggaggt gctgtgggcc gtgcgcgacg ccgtcaccgg cgactgatgc
     6181 cggtcggggc gcggcagggc ccccgaccga atgggaggcg gtcgggggcc ttcggagcct
     6241 gagggacggg cagccctagg actccgcagc agcgaccgca gcctcgaggg atgcgagcgc
     6301 ctgctggccg cgactggtgg tctcggcggt gatgcggcag tgcttgtcgt ggtccccgtg
     6361 gctggcggcc acggcggagg tctcggccag catcttggcc gtggcgctca gcagcgcctg
     6421 ggtgccctgc acggtggggc cggtgcggga caggaccttg atcagggcgg tggcgacgtc
     6481 ggcggcgtcg aggcaggccc gcacgcactc ggtcttgtcg gccacgtcgg gctcggacag
     6541 gcaggcgtcg gcgcacgagg tggtggcgct gacgacgtcg tgcatcgcgt cgatggcgag
     6601 ggcgagggcg gcgtgatcca cacctcgttc gtccgagcgg gcttcgagca tctgggcagc
     6661 gacgttcatg tcagcgttcc tgccccaact gctggggcgt cagaacccgc cgcctgcccg
     6721 ccggtcgcgc cgcggtagac agagcacgtg gatcccgagg tgctcgcccg acggcgtcgt
     6781 gagtacgagg cgcagggctt tgacgtggcc gacgccgacc ccgacccgct ggtgcagttc
     6841 caccgctggt tcgacgccgt cgacgagccc atggacgagc cccacgccat ggttctcgcc
     6901 acggtgcaac cggacggcac gccgtcggtc cgcacggtgc tcctgcgcgg cgtcgacgag
     6961 cgggggctgg gcttcttcac caactacgag agcgccaagg ccgacgcgat cgaggcccaa
     7021 ccggtcgtcg agtgcctgtt cctgtggctg cccgtgcacc gccaggtccg ggtgcggggt
     7081 cgggccgaga aggtgtcggt cgaggagtcc gacgagtact tcgccagccg gccgcgggga
     7141 tcccagctcg gtgcgtgggc gtcgccgcag tcccaggtgg tctccggtcg cgaggagctc
     7201 gagcgctacc tcgccgccgc tgaggagcgc ttcacccacg aggtgccccg gccgccgcac
     7261 tggggcggct accgggtggt gcccgacggg tgggagttct ggcagggccg tcccggccga
     7321 ctccacgacc gggtgcgcta ccggcgcgag ggcggcgcct gggtcaggga gcgcctggcc
     7381 ccctgagcgc gtcgatggcg gcgacgaggc cgtcgaccag gcgctcggtg tgcgg
//
LOCUS       DATISU010000361         1938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_168195, whole genome shotgun sequence.
ACCESSION   DATISU010000361 DATISU010000000
VERSION     DATISU010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168195"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..815
                     /gene="tsf"
                     /locus_tag="VLR27_14340"
     CDS             21..815
                     /gene="tsf"
                     /locus_tag="VLR27_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HSP04687.1"
                     /translation="MAEFTAKDVQALRQATGAGMMDAKKALVDAEGDFDAAAKKLREK
                     GLAKAATRTDRENVEGAVAVAETDTAAAIVQLKSETDFSAKSDDFVALTQDIASAVAA
                     EGPGAVEGFTARIDDLKVTLKENIDLGTVTHVEKGAGQVVDTYVHVQDGRGVNGVVTV
                     LDGGDAELAHDISIHIAFTKPAYLRRDEVPQTAIDDERSTLEEITRKEGKPEQALPKI
                     VEGRLDGWFRDQVLLEQKFVRDEKQTIEQLLGGASLVRFEQVVVGG"
     gene            875..1564
                     /gene="pyrH"
                     /locus_tag="VLR27_14345"
     CDS             875..1564
                     /gene="pyrH"
                     /locus_tag="VLR27_14345"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HSP04688.1"
                     /translation="MKLSGEAFAGPDGFGIDGDTVGQIAEEVLDCRRLGVDVAVVVGG
                     GNLWRGMTGAGKGMDRAQADYMGMLGTVMNALALQDILERKGQPTRVQTAIHMAQIAE
                     PYIRRRAIRHLEKGRVVIFGAGNGNPFFTTDTTAALRAVEIDAEAVLKGSHSGVDGIY
                     TADPRTDPEATKLDHLSYLDVLNRGLAVMDSTAITLCMDNDLPILVFDLMTPGNVRSI
                     LQGQAIGTLVS"
     gene            1561..>1938
                     /locus_tag="VLR27_14350"
     CDS             1561..>1938
                     /locus_tag="VLR27_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HSP04689.1"
                     /translation="MNDDLVSMILDDAATKMAAAVARTREEFSAVRTGRASPAFVEKL
                     QVEYYGTDVALQQLAGFAVPEARTLLITPFDKDAIPGIEKAIQQSDLGLNPSSDGVVI
                     RLNFPQLTEERRRDYVKVVKQKAE"
BASE COUNT          341 a          702 c          639 g          256 t
ORIGIN      
        1 cacggagtcg tagaaccccc atggcagagt tcaccgccaa ggacgtccag gccctgcgcc
       61 aggcaaccgg cgccggcatg atggacgcca agaaggcgct cgtcgacgcc gagggcgact
      121 tcgacgccgc cgccaagaag ctgcgcgaga agggcctggc caaggcggcc acccgcaccg
      181 accgcgagaa cgtggaaggc gccgtggccg tggccgagac cgacaccgcc gccgccatcg
      241 tgcagctgaa gtccgagacc gacttctccg ccaagtccga cgacttcgtc gccctcaccc
      301 aggacatcgc cagcgcggtg gccgccgagg gcccgggtgc cgtcgagggc ttcaccgccc
      361 gcatcgacga cctcaaggtg acgctgaagg agaacatcga cctcggcacc gtcacccacg
      421 tcgagaaggg tgccggccag gtcgtggaca cctacgtcca cgtccaggac gggcgcggcg
      481 tcaacggcgt cgtcaccgtg ctcgacggcg gcgacgcgga gctggcgcac gacatctcga
      541 tccacatcgc cttcaccaag ccggcctacc tgcgtcgcga cgaggtgccc cagaccgcga
      601 tcgacgacga gcgctccacc ctcgaggaga tcacccgcaa ggagggcaag cccgagcagg
      661 cgctgcccaa gatcgtcgag ggtcgcctcg acgggtggtt ccgcgaccag gtgctcctcg
      721 agcagaagtt cgtgcgcgac gagaagcaga ccatcgagca gctgctcggc ggtgcatccc
      781 tcgtccgctt cgagcaggtc gtcgtcgggg gctgagcatc tccgccgctg acgagaccga
      841 cgggtccgcg aagccccgtt ggggccgcgt cctgctgaag ctgtccggcg aggccttcgc
      901 cggcccggac ggcttcggca tcgacggcga caccgtcggc cagatcgccg aggaggtgct
      961 ggactgccgg cgcctcggcg tcgacgtggc cgtggtcgtc ggcggcggca acctgtggcg
     1021 cgggatgacc ggcgccggca agggcatgga ccgggcccag gccgactaca tgggcatgct
     1081 cggcacggtc atgaacgccc ttgcgctcca ggacatcctc gagcgcaagg gtcagcccac
     1141 ccgcgtccag accgccatcc acatggccca gatcgccgag ccctacatcc ggcgtcgcgc
     1201 catccgtcac ctcgagaagg ggcgggtcgt catcttcggc gccggcaacg gcaacccgtt
     1261 cttcaccacc gacaccacgg ccgcgctacg agccgtggag atcgacgccg aggccgtgct
     1321 caagggcagc cactccggcg tcgacggcat ctacaccgcc gatccccgca cggatcccga
     1381 ggcgaccaag ctcgaccacc tgagctacct cgacgtgctc aaccggggcc tggccgtcat
     1441 ggactccacg gcgatcacgc tgtgcatgga caacgacctg ccgatcctcg tgttcgacct
     1501 catgaccccg ggcaacgtgc ggtccatcct gcagggtcag gcgatcggta cgctggtttc
     1561 gtgaacgacg acctcgtgtc gatgatcctc gacgacgctg cgaccaagat ggcggccgcc
     1621 gtcgcgcgca cccgtgagga gttctcggcg gtgcgcacgg gccgggcatc gcctgccttc
     1681 gtcgagaagc tgcaggtcga gtactacggc accgacgtgg ccctccagca gctggccggc
     1741 ttcgccgtgc ccgaggcgcg cacgctgctc atcaccccgt tcgacaagga cgcgatcccg
     1801 gggatcgaga aggccatcca gcagtccgac ctcgggctca acccctcgag cgacggcgtg
     1861 gtcatccgac tcaacttccc gcagctcacc gaggagcggc ggcgggacta cgtcaaggtc
     1921 gtgaagcaga aggcagag
//
LOCUS       DATISU010000362        10038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_819499, whole genome shotgun sequence.
ACCESSION   DATISU010000362 DATISU010000000
VERSION     DATISU010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10038
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819499"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2455)
                     /locus_tag="VLR27_14355"
     CDS             complement(<1..2455)
                     /locus_tag="VLR27_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSP04690.1"
                     /translation="MPETRPDIVVLLTDEERAAPPYETEALRAWRRRALPGWRWFDEH
                     GVRFTRHHTGSLACVPSRPTLLTGQYPDLHGVSQTDGLGKMADDSRMRWLPPDEVPTI
                     GSWFRAAGYDTHYDGKWHVSHADIADPATGRPLATNDAEGNVDPAAVQAYLDADPLGA
                     YGFSGWIGPEPHGAPMANSGLVRDPLIADRVVAWLEDRYARRRAGDADAQRPFLLVAS
                     FVNPHDIVLFPAWVRGRWPLEPSPLDPPPVPPSPTDGEDLRDKPAAQIAYRAAYPSGY
                     GPAPAVARIYRRGAQRYRDLYHRLHVEVDGPLDRVRRAVTDGGSEQAVLVRTSDHGEL
                     LGAHGGLHQKWFNLYDEATRVPFTVARVGERATAPRTVDAPTSHVDLVPTLLAAAGID
                     VEATARTLATTFSEVHPLPGRDLLPVVDGAEPDPDRVVYLQTRDNMLEGDSGASGVAR
                     RLGRVVRPPAPLRIRVPTHVASSFEGVVARVPEEVVPGGAGHRWKLVRTFDDPSTWTE
                     PGVRHLADTGPAGPAYRTEPLEDQWELYDLDADPIELRNRWPDPDAAEVLAHLRDVLK
                     AEAARSIPERNQPLVAARRHPTERPAKTPPRPALALRRIVQRLGMHPEDRDADPTIDR
                     TGRRALVVCTNHGVLDVGKPTGVFASEMTVPYYAFRDAGMLVEVASPAGGAVPIDPQS
                     LKPVLRCAADDRFLADPELRAKVTDSLAIADVDISGYDLVYLAGGWGAAFDFGTSQAL
                     GDQLTRAHELGLPIGGVCHGPLALRLATAPDGRPLVEGRRISCVTDKQVHELGIASTP
                     MHPETELRALGARFESRHRF"
     gene            complement(2448..2903)
                     /locus_tag="VLR27_14360"
     CDS             complement(2448..2903)
                     /locus_tag="VLR27_14360"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSP04691.1"
                     /translation="MSAEVSRTAIVAAPVGVVWDLLADFGSLARWVPEVEHSCRLHAG
                     PVGIGTVRRVQVGRTTLLETITTWSPPDHLGYDITGLPPALRAVRNDWHLRPVDAGTE
                     VTVTTTVDAGPRPPQRLIAGIVARRMAATSDQMLAGLTAALTSGDRTRA"
     gene            complement(2900..3784)
                     /locus_tag="VLR27_14365"
     CDS             complement(2900..3784)
                     /locus_tag="VLR27_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04692.1"
                     /translation="MTTDGAAWRALTADAALASHRLVGWIYWDPTAVANYAAFGVPDG
                     AGYYIATRAASIAAAGDDVVTAVFGSIHPDFVSVCLDLCREHTTFDAAAEARDAAVEP
                     GLREIAPDVCDGLAELGPHLWSAADELVLTGRPLFASLLRRRREDQPLLSAWLAVNCI
                     REWRGDTHWAIHAAEGIDGTMAGLLDGAWRGHDDDWLPRSRGADDGALTAALTRLEAR
                     GLATGGRVDQAGIAYRQELEDRLDDLCAPGWQALGEARTRRLVQLVEPHGAAFVERID
                     RTAGPRWMPAARDRTVRT"
     gene            complement(3781..4692)
                     /locus_tag="VLR27_14370"
     CDS             complement(3781..4692)
                     /locus_tag="VLR27_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HSP04693.1"
                     /translation="MQTLRTPDDAFAGLPDFPFEPHYVEVDDGEGGTIRIHHLDEGPA
                     DAAPVLLMHGEPTWSFLYRHVIPGLVEAGHRVIAPDLVGMGRSDKPTEQSDHTYARHV
                     EWMRQALFDRLDLRDITFFGQDWGSLVGLRLVALDPDRYARVAIGNGGLPTGHGPASE
                     AFLAWQKFSQETEHFPVGRIIDGGCTTTLSPEVIAAYDAPYPDDSYKAAVRVFPSLVP
                     TSSDDPATPDQEAAWRVLEQFEQPFLLCFSDADPITKGGDAPFLAKVPGAQGQAHTTI
                     EGGGHFLQEDRGPELARVLAEFIETTS"
     gene            complement(4717..6978)
                     /locus_tag="VLR27_14375"
     CDS             complement(4717..6978)
                     /locus_tag="VLR27_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HSP04694.1"
                     /translation="MRADQFRGRIGPTIAESEPWFDEPPHPGEGAPDIVVVLLDDTGY
                     AQLGCFGSDIDTPNIDALAAGGVQFTNFHVTPLCSPTRAALLTGRAPHAVGMRAVSNF
                     RTGFPNQLGHISNHAGTVAEVLHDHGYATFCVGKWHLAPMEQCSAAGPFDQWPLARGF
                     DRFYGFLEGETDQFHPELVADNHPVEPPGGPEDGYHLSEDLVDQALRMIADSTGVRPD
                     RPVFTYLAFGATHAPHQAPPAYLDKYRGRFDEGWDVVRERWFRRQLELGVIPPDTRLA
                     PRNPGVEPWDDLPDDQRRLAARLQEAFAAFLDHTDVQIGRFVDGLRRLGRLENTILVV
                     LADNGASQEGGPYGVLHEMKFFNGILETPAEAVARIDDIGGPRSHTNYPWGWAQCGNT
                     PFRWYKQNTHEGGVHVPMVVHWPAGIDAAQRGTKRDQFAFVADIAPTLYDVVGIEPPT
                     VRNGLDQLPVTGHSFAAALHDPEAPPTNTVQYFENAGSRAIVADGWKAVTKHVAGADY
                     DTEPWELHHLAADPSETEDLAQREPDRLRQLVDLWWAEAERHGVLPLDDRTIELFGAR
                     FREHSPHPPDRRYVYRPPMSPLPGQAGAAIAGRSFDLTATVTHEPAAEGVLFATGTEN
                     SGLSLFVQAGRLVLDYNAFGDHTIVESDAAIPAGEVALGVRLRRLDGFAGTATLTVDG
                     VDAGHAELSLFMRMMSSIGPSIGADHGSAVSDRYDAPFAYDGTLHEIVIQASPDRYGD
                     TAATTARAEMSRQ"
     gene            7084..7686
                     /locus_tag="VLR27_14380"
     CDS             7084..7686
                     /locus_tag="VLR27_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP04695.1"
                     /translation="MSEQTVTTDGRRARRERNRAAVIDAVFELIVDDHVPPAVESIAA
                     RAGVSVSSVFRYFDSLDDLLEQTVARYFERFADLFAVPPVPDGDLDDRIATLVAARLD
                     LYEAIGPIARIARLRATEQPRLATVLADTRRTFEGQVRSHFEPDLTGRSRTEVDDRVA
                     LVDCLTAFESWDLLTGTHGRDRHLVERAWTSGLRALLVPA"
     gene            complement(7762..8805)
                     /locus_tag="VLR27_14385"
     CDS             complement(7762..8805)
                     /locus_tag="VLR27_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HSP04696.1"
                     /translation="MPIARSRFRRTAPAAALLGAFALLTVTASPAGADPIGTGEASAF
                     GGHIQLTDQDVVPPTPLAESDLAGEDVTETLVDIPADPVAVSGTLNADAAVHAESDIA
                     SQLTQVTQDVEGPYNASGVALIEGAEVLVDSIPDEVSLVTADVIRAEAVAVCRGGTVE
                     YSANSEIINLQLGGEDIPLDAPLSDLLDAIGDGLEESTLNQVLDIQRNVVTETDDGIA
                     VDALVVTVLAAAGDTPLATVRLGHAEVGGVTCGALTECSDGADNDGDGVIDADDPGCH
                     TDGDATNPDSYDPTDDSEADEPTGGAIAPGGATPSAERLPATGGDAVSTAGLAAVMAA
                     GALGVGALRRRFG"
     gene            complement(8985..>10038)
                     /gene="guaA"
                     /locus_tag="VLR27_14390"
     CDS             complement(8985..>10038)
                     /gene="guaA"
                     /locus_tag="VLR27_14390"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HSP04697.1"
                     /translation="GPYGVQFPPEVVHTQMGQDLLKHFLYEACGCRPTWTMSSVIETQ
                     VEAIRAQVGAGRAICGLSGGVDSAVAAALVHKAIGHQLTCVFVDTGLMRKDEGDQVVE
                     TFKRNFGIELIHVRAADRFFEKLAGVTDPEDKRKAIGELFIRIFEDAKGGITDADHLV
                     QGTLYPDVIESGGGAKAATIKSHHNVGGLPDDMDFDLVEPLRDLFKDEVRRVGEELGL
                     PEEIVWRQPFPGPGLGVRIIGEVTPDKVELLQEADAIVRAELHKAGMDREIWQAFAVL
                     PDIRAVGVMGDERTYGYPCIIRAVTSDDAMTADWARLPYDLLEKMSSRIINEVPGINR
                     VAYDITSKPPGTIEWE"
BASE COUNT         1310 a         3402 c         3896 g         1430 t
ORIGIN      
        1 ggaagcggtg ccgggactcg aacctcgcgc cgagcgcccg cagctcggtc tcggggtgca
       61 tcggcgtcga ggcgatgccg agctcgtgca cctgcttgtc ggtgacgcag ctgatccgcc
      121 ggccctcgac cagcggccga ccgtcgggtg cggtcgcgag ccggagcgcg agcgggccgt
      181 ggcacacgcc gccgatgggc agccccagct cgtgggcccg ggtgagctgg tcgccgaggg
      241 cctgggacgt gccgaagtcg aaggccgcgc cccacccacc ggcgaggtac acgaggtcgt
      301 agccgctgat gtcgacgtcc gcgatggcca gcgagtcggt caccttcgcc cgcagctccg
      361 ggtcggccaa gaaccggtcg tccgccgcgc agcgcagcac gggcttcagc gactgcgggt
      421 cgatcggcac cgccccgccg gcggggctgg cgacctcgac cagcatcccg gcatcgcgga
      481 acgcgtagta cggcacggtc atctcgctgg cgaacacgcc ggtgggcttg ccgacgtcga
      541 gcaccccgtg gttggtgcac acgacgaggg cgcgccgacc ggtccggtcg atggtcgggt
      601 cggcgtcgcg gtcttccggg tgcatgccga ggcgctggac gatgcgccgc agcgcgaggg
      661 ccggccgggg cggggtcttc gccgggcgct cggtcgggtg gcggcgagcg gcgaccagcg
      721 gctggttgcg ctccgggatg ctccgggccg cctccgcctt cagcacgtcg cgcaagtggg
      781 cgagcacctc cgccgcgtcc gggtccggcc agcggttgcg gagctcgatc gggtcggcgt
      841 cgaggtcgta gagctcccac tggtcctcga gcggctcggt ccggtacgcc ggaccggctg
      901 gcccggtgtc ggccaggtgg cgcaccccgg gctcggtcca ggtgctgggg tcgtcgaagg
      961 tccgcaccag cttccagcgg tgaccggcgc cgccgggcac gacctcctcg gggacgcggg
     1021 cgaccacgcc ttcgaagctc gaggcgacgt gggtgggcac gcggatgcgc agcggcgccg
     1081 gcggacggac cacgcgaccc aggcggcggg ccacgccgga ggcacccgag tcgccctcga
     1141 gcatgttgtc tcgggtctgg aggtagacga cgcggtccgg atcgggttcg gcgccgtcga
     1201 ccaccggcag caggtcgcga ccgggcagcg ggtggacctc ggagaaggtg gtggcgagcg
     1261 tgcgggcggt ggcctcgacg tcgatgccgg cggcagcgag cagcgtcggc accaggtcga
     1321 cgtgggaggt cggtgcgtcg acagtgcgcg gcgccgtggc tcgctcgccg acccgggcga
     1381 cggtgaaggg gacgcgtgtg gcctcgtcgt agaggttgaa ccacttctgg tgcaggcccc
     1441 cgtgcgcgcc gaggagctcg ccgtggtcgc tggtccgaac caggacggcc tgctcggagc
     1501 cgccgtcggt gacggcccgg cgcacgcgat cgagcgggcc gtcgacctcg acgtgcaacc
     1561 ggtggtacag gtcccggtag cgctgggcgc cgcgccggta gatgcgggcg acggccggag
     1621 ccggcccgta ccccgacggg taggccgccc ggtaggcgat ctgggcggca ggcttgtccc
     1681 gcaggtcctc tccatcggtc ggcgagggcg gcacgggcgg ggggtcgagc ggcgacggct
     1741 cgagcggcca gcgaccccgc acccaggccg ggaaaagcac gatgtcgtgg gggttcacga
     1801 agctggcgac gagcaggaac ggtcgctggg catcggcgtc tccggcgcgc cgtcgggcgt
     1861 agcgatcctc cagccaggcg accacccggt cggcgatgag cgggtcgcgc acgagcccgc
     1921 tgttggccat cggggcgccg tgcggttcgg gaccgatcca gccggagaag ccgtaggcgc
     1981 cgagcggatc ggcatccagg taggcctgca cggcggcggg gtcgacgttg ccctcggcgt
     2041 cgttcgtggc gagcggtcgg ccggtggccg ggtcggcgat gtcggcgtgg gacacgtgcc
     2101 acttgccgtc gtagtgcgtg tcgtagcccg cggcccggaa ccagctgccg atcgtgggca
     2161 cctcgtcggg cggcagccac cgcatgcggg agtcgtcggc catcttgccg aggccgtcgg
     2221 tctggctgac cccgtgcagg tccgggtact gcccggtgag cagcgtcggg cggctgggca
     2281 cgcaggcgag cgagccggtg tggtgtcgcg tgaaccgcac gccgtgctcg tcgaaccagc
     2341 gccacccggg cagggcgcgc cggcgccacg cccgcagcgc ctcggtctcg tacggcggcg
     2401 cggcgcgctc ctcgtcggtg aggagcacca cgatgtctgg gcgggtctca ggcacgggtg
     2461 cggtctccgg aggtgagggc agcggtgagg ccggcgagca tctggtccga ggtggccgcc
     2521 atgcggcggg cgacgatgcc ggcgatcagg cgctggggtg gccggggtcc ggcgtcgacg
     2581 gtggtggtga cggtgacctc cgtgcccgcg tccacgggtc ggaggtgcca gtcgttgcgc
     2641 accgcccgca gcgccggcgg caggccggtg atgtcgtagc cgaggtggtc gggcggcgac
     2701 cacgtggtga tcgtctcgag cagggtcgtg cgcccgacct gcacccgtcg gaccgtgccg
     2761 atgcccaccg gcccggcatg cagtcggcag gagtgctcca cctccggcac ccagcgggcc
     2821 agcgacccga agtcggcgag caggtcccac acgactccga cgggtgccgc cacgatggcg
     2881 gtgcgcgaca cctctgcgct cacgtgcgca ccgtccgatc gcgtgccgcc ggcatccacc
     2941 gggggccggc ggtccggtcg atgcgttcga cgaacgccgc accgtgcggt tcgaccagct
     3001 ggacgagccg gcgggtccgc gcctcgccga gggcctgcca acccggcgcg cagaggtcgt
     3061 cgaggcggtc ctcgagctcc tggcggtagg cgatgccggc ctggtccacc cggccgccgg
     3121 tggcgagccc ccgggcctcg aggcgggtca gggcggcggt cagcgccccg tcgtcggccc
     3181 cccgggagcg cggcagccag tcgtcgtcgt ggccgcgcca ggcgccgtcg agcagcccgg
     3241 ccatggtgcc gtcgatgccc tccgcagcgt ggatcgccca gtgcgtgtcc ccgcgccact
     3301 cgcggatgca gttgacggcc agccaggccg acagcagcgg ctggtcctcc cgcctccggc
     3361 gcaacagtga tgcgaagagc gggcgcccgg tgagcacgag ctcgtcggcg gcagaccaca
     3421 ggtgcggacc cagctcggcg aggccgtcgc acacgtcggg ggcgatctcc cgcaggccgg
     3481 gctcgacggc ggcatcgcgc gcctcggcgg cggcatcgaa ggtcgtgtgc tcgcggcaca
     3541 gatcgaggca gacggagacg aagtccgggt ggatcgaccc gaacaccgcc gtcaccacgt
     3601 cgtcgcccgc ggcggcgatc gaagcggctc gggtcgcgat gtagtagccg gcaccgtctg
     3661 ggaccccgaa tgctgcgtag ttcgccacgg cggtggggtc ccagtagatc cagccgacga
     3721 ggcgatgcga cgcgagcgcg gcgtccgctg tgagcgcccg ccaggcggcg ccgtccgtgg
     3781 tcacgacgtg gtctcgatga actcggcgag cacgcgggcc agctcggggc cgcggtcctc
     3841 ctgcaggaag tgcccgccgc cctcgatcgt ggtgtgggcc tgcccctggg caccgggcac
     3901 cttggccagg aacggtgcgt cgccgccctt ggtgatcggg tcggcgtcgc tgaagcacag
     3961 caggaacggc tgctcgaact gctcgagcac ccgccaggcc gcctcctggt ccggcgtggc
     4021 cgggtcgtcc gagctcgtgg gcaccaggct cgggaagacc cgcaccgcgg ccttgtagga
     4081 gtcgtcgggg tagggcgcgt cgtaggcggc gatgacctcc gggctgagcg tggtcgtgca
     4141 gccgccgtcg atgatccgtc ccaccgggaa gtgctcggtc tcctgggaga acttctgcca
     4201 ggccaggaag gcctcgctcg ccgggccgtg gccggtgggc aggccgccgt tgccgatggc
     4261 gacgcgggcg tagcggtcgg ggtcgagcgc gacgaggcgc aggccgacca gcgaccccca
     4321 gtcctggccg aagaaggtga tgtcacgcag gtcgagccgg tcgaagaggg cctggcgcat
     4381 ccactcgacg tggcgggcgt aggtgtggtc ggactgctcg gtcggcttgt cgctgcggcc
     4441 catgccgacc aggtccggag cgatcacgcg gtggccggcc tcgaccaggc cggggatgac
     4501 gtggcggtag aggaagctcc aggtcggctc gccgtgcatg agcagcaccg gcgcggcgtc
     4561 ggccggcccc tcgtcgaggt ggtggatgcg gatggtgccg ccttcgccgt cgtcgacctc
     4621 cacgtagtgg ggttcgaacg ggaagtcggg gaggccggcg aaggcgtcgt cgggggtgcg
     4681 gagggtctgc atggtgttcc tcgtgggtgt cagcggctac tggcgggaca tctcagcgcg
     4741 tgcggtggtg gcggccgtgt cgccgtaccg gtcggggctg gcctggatga cgatctcgtg
     4801 cagggtgccg tcgtaggcga acggcgcgtc gtagcggtca gagaccgccg agccgtggtc
     4861 ggcgccgatg ctcggcccga tggaggacat catgcgcatg aacagggaga gctcggcgtg
     4921 gccggcgtcg accccgtcga cggtgagcgt cgcggtgccg gcgaagccgt cgaggcggcg
     4981 gagccggacg ccgagggcga cctcacccgc cgggatcgcc gcgtccgact ccacgatcgt
     5041 gtggtcgccg aacgcgttgt agtcgagcac caggcgaccg gcctgcacga agagggagag
     5101 cccggagttc tcggtgccgg tggcgaacag cacgccctcc gccgccggct cgtgggtgac
     5161 ggtggcggtc aggtcgaagc tgcgtccggc gatggcggcg ccggcctgcc cggggagggg
     5221 cgacatcggc ggccggtaca cgtagcggcg atccggcggg tgtggggagt gctcgcggaa
     5281 gcgggcgccg aacagctcga tggtgcggtc gtcgaggggc agcacgccgt ggcgttcggc
     5341 ctcggcccac cagaggtcga cgagctggcg cagccggtcc ggctcgcgct gggcgaggtc
     5401 ctcggtctcg gacgggtccg ctgccaggtg gtgcagctcc cacggttcgg tgtcgtagtc
     5461 ggccccggcc acgtgcttgg tcaccgcctt ccacccgtcg gcgacgatgg cgcggctgcc
     5521 ggcgttctcg aagtactgga cggtgttcgt cggcggcgcc tcggggtcgt ggagcgcggc
     5581 ggcgaacgag tgaccggtca ccgggagctg gtcgagcccg ttgcgcaccg tcggcggctc
     5641 gatgccgacc acgtcgtaga gcgtcggggc gatgtcggcc acgaaggcga actggtcccg
     5701 cttcgtgccc cgctgggcgg cgtcgatccc cgctggccag tgcaccacca tcgggacgtg
     5761 gaccccgccc tcgtgggtgt tctgcttgta ccagcggaac ggggtgttgc cgcactgggc
     5821 ccagccccac gggtagttgg tgtggctgcg gggtccgccg atgtcgtcga tgcgggccac
     5881 cgcctcggcg ggggtctcca ggatgccgtt gaagaacttc atctcgtgga ggacgccgta
     5941 gggcccaccc tcctgcgacg cgccgttgtc ggcgagcacg acgaggatcg tgttctccaa
     6001 ccggccgagg cggcgcagcc cgtcgacgaa ccggccgatc tggacgtcgg tgtggtcgag
     6061 gaacgcggcg aaggcctcct ggaggcgagc ggcgaggcgg cgctggtcgt cgggcaggtc
     6121 gtcccacggc tcgacgccgg ggttgcgggg cgcgagccgg gtgtcgggcg ggatcacgcc
     6181 gagctcgagc tggcgccgga accagcgctc ccggaccacg tcccagccct cgtcgaagcg
     6241 gccccggtac ttgtcgaggt acgccggcgg ggcctggtgg ggggcgtggg tggcgccgaa
     6301 ggcgaggtag gtgaagacgg gccggtcggg gcgcacgccc gtggagtcgg cgatcatccg
     6361 cagcgcctgg tcgacgaggt cctcggacag gtggtagccg tcctcgggcc cccccggcgg
     6421 ctcgaccggg tggttgtcgg cgaccagctc ggggtggaac tggtcggtct caccctcgag
     6481 gaagccgtag aaccggtcga acccgcgcgc gaggggccac tggtcgaagg gtccggccgc
     6541 cgagcactgc tccatgggcg ccaggtgcca cttgccgacg cagaaggtgg cgtagccgtg
     6601 gtcgtggagg acctcggcga cggtgccggc gtggttggag atgtggccga gctggttggg
     6661 gaacccggtg cggaagttcg acacggcccg catcccgacc gcgtggggtg cccggccggt
     6721 gagcagcgcc gcccgggtcg gcgagcacag gggggtcacg tggaagttcg tgaactgcac
     6781 cccacccgcc gccagcgcgt cgatgttggg ggtgtcgatg tcggagccga agcagccgag
     6841 ctgggcgtac ccggtgtcgt cgaggaggac gaccacgatg tcgggcgccc cctcccccgg
     6901 gtggggcggt tcatcgaacc acggttcgga ctcggcgatc gtcgggccga tgcggccccg
     6961 gaactggtct gctcgcacct ggacctcccc ggtctcgccc catcgaaaca cctgagagtt
     7021 ccactgtcaa tacattctgc gagccgcgct cgcaggaggt cgctcggcgc taccgtggcc
     7081 acggtgtccg agcagaccgt caccaccgac gggcggcgtg cccgccgcga gcgcaaccgc
     7141 gccgccgtca tcgacgccgt gttcgagctg atcgtcgacg accacgtgcc acccgccgtg
     7201 gagtcgatcg cagcgcgcgc cggcgtgtcg gtgtcatcgg tcttccgcta cttcgacagc
     7261 ctcgacgacc tgctcgagca gaccgtggcc cgctacttcg agcgcttcgc cgacctgttc
     7321 gcggtcccac ccgtgccgga cggcgacctc gacgaccgca tcgccaccct cgtcgccgct
     7381 cgcctcgacc tctacgaggc catcggcccc atcgcccgca tcgcccggct gcgggccacc
     7441 gagcagcccc gcctcgccac ggtcctggcc gacacgcgcc ggaccttcga gggccaggtg
     7501 cggtcgcact tcgagcccga tctgaccgga cgcagccgga ccgaggtcga cgaccgcgtc
     7561 gcgctggtcg actgcctcac cgccttcgag tcctgggatc tgctcaccgg cacccacggg
     7621 cgggaccgcc acctcgtcga gcgggcgtgg acgtcggggc tgcgcgccct gctcgtcccg
     7681 gcctgaaccg cacctgagcc cggacgcgac tcgggcggcc cctccgctgg agggaccgcc
     7741 cgggcacgtc gttcggaggc gtcagccgaa gcgtcgacgc agggcgccga caccgagggc
     7801 gccggcggcc atcacggcgg ccaggcctgc ggtgctgacg gcgtcaccac cggtggcggg
     7861 gaggcgctcc gcggagggcg tggcaccgcc gggggcgatg gccccgccgg tcggctcgtc
     7921 ggcctcgctg tcgtcggtgg ggtcgtagga gtccgggttc gtggcgtcac catcggtgtg
     7981 gcacccggga tcgtcggcat cgatgacgcc gtcaccatcg ttgtcggcgc cgtcggagca
     8041 ctcggtgagc gcaccgcagg tcacgccccc cacctcggcg tggccgaggc ggacggtggc
     8101 gagcggggtg tcgccggcag cggccagcac ggtgacgacc agggcgtcca cggcgatgcc
     8161 gtcgtcggtt tcggtcacca cgttgcgctg gatgtcgagc acctggttga gggtgctctc
     8221 ctccaggccg tcgccgatgg catcgagcag gtcggacagc ggcgcgtcga gcgggatgtc
     8281 ctcgccaccg agctggaggt tgatgatctc ggagttggcg gagtactcga cggtcccacc
     8341 gcggcacact gcgaccgctt cggcccggat cacgtcggcg gtgacaaggg agacctcgtc
     8401 ggggatgctg tcgaccagca cctcggcacc ttcgatcagg gccacgccgg aggcgttgta
     8461 ggggccctcg acgtcctggg tgacctgggt cagctgcgag gcgatgtcgg actcggcgtg
     8521 gacggcggcg tcggcgttga gcgtgccgga gacggcgacc gggtcggccg ggatgtcgac
     8581 gagggtctcg gtgacgtcct cgcccgccag gtcggactcg gccagcggcg tggggggcac
     8641 gacgtcctgg tcggtcagct ggatgtggcc accgaaggct gacgcctcgc cggtcccgat
     8701 gggatcggcc ccggccgggg acgccgtgac ggtcagcagg gcgaaggccc cgagcagcgc
     8761 ggccgcggga gcggtgcgcc ggaatctgga tcgagcgatg ggcatgtgtt cctccgaagg
     8821 gactgtggtt ccgccgagca gggagccact tcctgtccga ccgtaggagt acttagacgg
     8881 cgaacgccgc gatacctacc tagatcggcg cggaatgacg caaagcgtgg acgaaaaggt
     8941 gcatttcgcc acggatcgtc acgaacggga cacgatccgg gcgactactc ccactcgatg
     9001 gtgccgggcg gcttcgaggt gatgtcgtag gcgacccggt tgatgcccgg cacctcgttg
     9061 atgatccggc tcgacatctt ctcgagcagg tcgtagggca ggcgggccca gtcggcggtc
     9121 atggcatcgt cggaggtgac ggcgcggatg atgcaggggt agccgtaggt gcgctcgtca
     9181 cccatcacgc ccacggcgcg gatgtcgggc agcacggcga aggcctgcca gatctctcgg
     9241 tccatgccgg ccttgtgcag ctcggcccgc acgatggcgt cggcctcctg cagcagctcg
     9301 accttgtccg gggtcacctc gccgatgatc cgcacgccga ggcccggccc ggggaacggc
     9361 tggcgccaca cgatctcctc gggcaggccg agctcctcgc ccacccggcg gacctcgtcc
     9421 ttgaacaggt cgcggagggg ttcgacgagg tcgaagtcca tgtcgtcggg cagaccgccc
     9481 acgttgtggt ggctcttgat ggtggccgcc ttggccccgc cgcccgactc gatcacgtcg
     9541 gggtagagcg tgccctgcac caggtggtcg gcgtcggtga tgccgccctt ggcgtcctcg
     9601 aagatgcgga tgaacagctc gccgatggcc ttgcgcttgt cctccgggtc ggtgacgccg
     9661 gccagcttct cgaagaagcg gtcggcggcc cggacgtgga tcagctcgat gccgaagttc
     9721 cgcttgaagg tctcgacgac ctggtcgccc tcgtccttgc gcatgaggcc ggtgtccacg
     9781 aacacgcagg tcagctgatg tccgatggcc ttgtgcacca gggcggcagc cacggcggag
     9841 tccacgccac ccgagagccc gcagatggct cggccggcgc cgacctgggc ccggatggcc
     9901 tcgacctggg tctcgatcac cgacgacatc gtccacgtgg gccggcaccc gcacgcctcg
     9961 tagaggaagt gcttgagcag gtcctggccc atctgggtgt ggacgacctc gggggggaac
    10021 tgcaccccgt aggggccc
//
LOCUS       DATISU010000363        13507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_75057, whole genome shotgun sequence.
ACCESSION   DATISU010000363 DATISU010000000
VERSION     DATISU010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 13507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13507
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75057"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VLR27_14395"
     CDS             complement(<1..696)
                     /locus_tag="VLR27_14395"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HSP04698.1"
                     /translation="MTDLLSISDLERGDIERLLDLTDHFAEVSTRPIPKVPALRGKTV
                     VSLFYEDSTRTRLSFETAAKRLSADTMNFSVSTSSVKKGESLRDTAQTIEAMGIDCVV
                     VRHKSSGVPWQLTRWVDASVVNAGDGWHEHPTQALLDCYTIRQQRGSLDGMHIAIVGD
                     VKHSRVARSDIWAFTKLGATVTVVAPPTLLPPAIDAYPVRVSHDIDAVLPTVDAVYLL
                     RMQRERMTEALLPS"
     gene            complement(693..1193)
                     /gene="pyrR"
                     /locus_tag="VLR27_14400"
     CDS             complement(693..1193)
                     /gene="pyrR"
                     /locus_tag="VLR27_14400"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HSP04699.1"
                     /translation="MDADDLRRATWRMAHEIIEANGGVDEVVLIGLQTGGVPLAERLQ
                     EALLEIEGVEVPVGKLDVAFHRDDIGLRPVLPEAVTDVPFHLDGVVVVLVDDVLFTGR
                     TIRAALDAITSFGRPRAIQLAVMVDRGHRELPIRPDYVGKNLPTRKDEAVDVHDEGVD
                     LGEVVK"
     gene            complement(1316..1798)
                     /locus_tag="VLR27_14405"
     CDS             complement(1316..1798)
                     /locus_tag="VLR27_14405"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP04700.1"
                     /translation="MLPTPPEGLRHWRPEDAPALEAAWADPDIARWNPPPDRLDADAW
                     IAGAEQRWEHRLALDLVIVPDVAGEVGLSGFTTDPARAELGVWVAAPHRRTGAARRAV
                     EAVTAWALDPDGLAVDQLWARTDPANLAAEALFDRLGWDRLGASDGKTIWSATTAVLR
                     "
     gene            complement(1779..2825)
                     /gene="nusB"
                     /locus_tag="VLR27_14410"
     CDS             complement(1779..2825)
                     /gene="nusB"
                     /locus_tag="VLR27_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HSP04701.1"
                     /translation="MSLPSTRREARERAVELLYEADTKSLHPEDVVASLPIRPDAYAL
                     ELAYGVADHQVEIDHVLDRYANRWPVKRMAAMDRMVLRLGVLELATQLEVPPGVCLSE
                     AVELGGRYGSTDDTPKFVNGILARVAEEVRDGDRPWMPIDVVVFDMDGVIRHWTAQAI
                     GRFQDAHDLPPDAVGSIAFSEPLYSDAMCGRLTAEEWAAEIGRRLAEAHDGVDADAGR
                     QMWLETEWRIDDEVVAIVSALRDAGQRVAVFSNATTKLEVDMGTMGIHELFHDICNSS
                     LIGLMKPDVASFEHVADEVLGVAPERLLFIDDRHENVVGALEAGWHAVQMRSAAGIAA
                     VLRRLQVPGAPDPA"
     gene            complement(2822..3385)
                     /gene="efp"
                     /locus_tag="VLR27_14415"
     CDS             complement(2822..3385)
                     /gene="efp"
                     /locus_tag="VLR27_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HSP04702.1"
                     /translation="MATVSTNDLKNGMCLDLPEGLVQVQDFQHVKPGKGGAFVRTTLK
                     NVRTGANIDKTFRAGEKVEQAKIDRSEYQYLYNDGSSYVFMDQETYDQIEVAPSNLGD
                     ATNYLVEQATAELMMYGDEIVGVELPPSVVLTVAETEPGLQGDRSTGGTKPATLETGY
                     VVQVPLFINTGEKLKIDTRTGQYQSRA"
     gene            complement(3438..4541)
                     /locus_tag="VLR27_14420"
     CDS             complement(3438..4541)
                     /locus_tag="VLR27_14420"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HSP04703.1"
                     /translation="MSPTDLAPLEVAPRADRVRAALDDAGCEALVVTDLTNVRWLTGF
                     TGSAGVLVVRPHDLVLVTDGRYQEQAADQLAAAGVTAEIEITRDTTDRLATVLDGIGP
                     VGLEADDVTWDTQQRWAEALPGSLIPTVGLLLGLRAVKDDGEVDRLQRAAEIADAALA
                     DVRHLLREQPTERDFARALESAMLDHGAQGPSFETIVASGPNGARPHARPSDRVIGAT
                     GEGELVVLDFGALCDGYHSDMTRTFVVGEPSATQARMLEVVRASQAAGVAAVRAGAPA
                     VGIDGACRAVIDEAGWSDAFVHGTGHGIGLVIHEEPRLSPESTALLAPGHCVTVEPGV
                     YLPEHGGVRIEDSVVVTATGCRPLTASPYDPEP"
     gene            complement(4538..5542)
                     /locus_tag="VLR27_14425"
     CDS             complement(4538..5542)
                     /locus_tag="VLR27_14425"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HSP04704.1"
                     /translation="MITVEVPVPGRSYPAVVGRGARRELAALLPEGTGRVAVVTQRGI
                     GMTADPGVEHRVFHIEAGEDAKTMRTVEYLCRQWATWGLTRADCVVAIGGGMVTDVAG
                     FAAASYHRGVPVVHVATTLLGMVDAAIGGKTGVNLPEGKNLVGAFWQPSAVVCDLEAL
                     DTLPPRELRGGYGELAKYRFLPGGEGLDDMELEEQVAQCIRIKAAIVAADEREGGLRA
                     ILNYGHTLAHALETAGEYDLRHGEAVAVGLLYAAALARRLGRISDERVALHHEIVSRY
                     ELPDRVPEGLDDDQLLSLMARDKKALDGLTFALDGDDGVEIVTGVDAEVARAALGDVR
                     "
     gene            complement(5550..6722)
                     /gene="aroC"
                     /locus_tag="VLR27_14430"
     CDS             complement(5550..6722)
                     /gene="aroC"
                     /locus_tag="VLR27_14430"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HSP04705.1"
                     /translation="MLRYLTAGESHGAALVVIVEGLPAGLPIQVEEVQGELARRRLGY
                     GRGPRMRFEQDEVTLVGGVRHGRTLGSPVAIEIANTEWTRSDKWHEEMSPAPGATKNP
                     ATQPRPGHADLAGMQKYGFDDARDVLERASARETAARVAAGTLAKALLSHLGISVLSH
                     VIQMGQARASAGIRPAPGDLGAVDEDEVRCFDPTASAAMVEEIKAAAKDGDSLGGVAE
                     VLAYGVPVGLGSHVHWDRKIDAALAQALMSIQAIKGVEIGAGFEVAGLRGSDAHDPIG
                     WAPDARDGRGDYVREGTRAGGVEGGITTGELLVARAAMKPLASLNRPSLKTVDVRTKE
                     ETVSFKERTDVTAVPAMGVVAETMVALVLATEATRKFGGDSLDELVRNHDAYVQTR"
     gene            complement(6790..7581)
                     /locus_tag="VLR27_14435"
     CDS             complement(6790..7581)
                     /locus_tag="VLR27_14435"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HSP04706.1"
                     /translation="MDVVAVVLAGIAGLVVGSLANRLIVREPGYVVTDPKDLPEGADE
                     SLLDELEPVPTDLAPVPVLAIARPGTWWRRWFPVTEVIMAATFAATVAELGWSAPTAA
                     ILFLVAALVTLAAVDLRVYRIPDRINFPSMGIGLGAIVLASLIDGAPGTIVGALIGGV
                     LYASMLFVFHLISPRGMGWGDVKLAWLMGFYLGWRGHEPGGALDLLLGPLRAVLLGAA
                     LGSILGVLSGGVYAIARRSTKVVFPYGPSLAAGCTIAVVWGITPL"
     gene            complement(7642..8724)
                     /locus_tag="VLR27_14440"
     CDS             complement(7642..8724)
                     /locus_tag="VLR27_14440"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HSP04707.1"
                     /translation="MALSGTLETFSLPDVLRLLSSTKKTGLLALDGDRGRGRVWVRDG
                     AIVAADADRAVEDAVEGVAFELLRFVDARFDFDSGAEPPAEREPRTVDEVLSEAEARL
                     VEWREIETVVPSLDVWVHMVAELDDERTIAPTEWRVLAQVGTGASGHALAERLEQGEY
                     DVCRQLRDLVEGGLVELTEAPTEAVVEAPVTTFATVEEPTFVEPALDPDAVSSLGTDL
                     ASFVAVGTVEEPYTVAAPVTVEEPVVEPEATYEPETSEAETTFEPDTAEAESFEPDTE
                     PEDDDDFASQLSQLSPKAAAAVEATWGGDEQAAADEPAADEPVETIEPVAEVEPVEPV
                     GADEPASGSDEIDQNLLLRFLSSTKN"
     gene            complement(8813..9631)
                     /locus_tag="VLR27_14445"
     CDS             complement(8813..9631)
                     /locus_tag="VLR27_14445"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HSP04708.1"
                     /translation="MIRTPTGATHLAGVIGWPVRHSRSPAIHNAAYEALGLDWVYVAL
                     PVAPGQVPAALAGMRALGLEGLSVTMPHKGAVAAEVDVLTADAAALGAVNSVRREGDR
                     LIGDNTDGGGFVDSLAEAGVDPAGRRCVVLGAGGAARAVVRALAVAGAAQVTVLNRDP
                     GRGEAAAALAGDVGAVGTTSDLGTADLLVQATPVGMGDDATLAADPDLLPAGAVVADL
                     VYHPLVTPLLAAAQERGLQTVDGLGMLVHQAARQLHAWTGRDAPVDVMRAAAAG"
     gene            complement(9628..10839)
                     /gene="mltG"
                     /locus_tag="VLR27_14450"
     CDS             complement(9628..10839)
                     /gene="mltG"
                     /locus_tag="VLR27_14450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HSP04709.1"
                     /translation="MNDDWTRDHDPADDWDTTTVHDHEGDYVEEEPWAEGEWDDWHPP
                     SHTGRKLFIAFLVLLALLAAGAFVAWNQVQDRIDPPGPPGATVELEIPSGSTTEDIGA
                     LLVDNDIITSSTIWNYWTRLQSVGPFQAGFYEFQENSSFDEAVAVLDEGPRPPQLERV
                     TIPEGLTVTEILDRLADPERGVERWSLEALQAAASSGEIRSQFQPEGESACIQEGGPP
                     WCALEGLLFPDTYEIDEDVDEAAFLGRLVAEMDARLAGLDIETRAAELGRTPYEVLVI
                     ASLIEAETRVPEERAKVARVVYNRLADGIPLGIDATSRYESVLAGRGRDSLDFESDSP
                     YNTRRVAGIPPTPIAAPGQASLEGALNPADGPWIYYVLQDEQGNHFFTESFSEFNQAK
                     AECARLGLGCG"
     gene            complement(10836..11270)
                     /gene="ruvX"
                     /locus_tag="VLR27_14455"
     CDS             complement(10836..11270)
                     /gene="ruvX"
                     /locus_tag="VLR27_14455"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HSP04710.1"
                     /translation="MRALALDLGSKRIGIALSSGTLATPYEVLARAGDRRRDHRAIAE
                     HVAETEAEVVVVGLPLSLDGSIGHAAERVLAECDQLAEALDVPIETWDERLSTVTAER
                     SLTEQQLRGPARRRVVDKVAAAVILQSWLDARPAEHPTEEPS"
     gene            complement(11278..>13507)
                     /gene="alaS"
                     /locus_tag="VLR27_14460"
     CDS             complement(11278..>13507)
                     /gene="alaS"
                     /locus_tag="VLR27_14460"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HSP04711.1"
                     /translation="DRIWITVHTSDDEAEDIWADAVGFPRERIQRLDKDNFWEMGDTG
                     PCGPSSELFWDFGPDEGPDGGPANPAAEERYVEFWNLVFPQYFRQPDGSLRDLDAPGI
                     DTGAGLERILGVLADSPSLYAADTLSVLTDRAQEVTGARFGGDRLHDIALRLIADHTR
                     TVAFLVADGVMPSNEDRGYVLRRVLRRAVYFSYMLGVEKPVLPPMIETCVELMGESYP
                     ELAESADSVLTIVSREEDRFRRTLRTGSTMLDTHIATIPEGGTVDGTVAFTLHDTHGF
                     PFEVTQEIAAERGYDVDEDAFRKAMADQRARAKAASKGGGVAVGDEVDAFRSVLDASG
                     PTEFVGREVDSTTTTVVGVVPGADDTVSIFLAATPFYAEAGGQVGDTGEITADGARAE
                     VLDTVYALPGLHRHIARITEGAFDLGDEVTASIDSDRRNAIRRHHTGTHVLHWALREV
                     LGDHVKQQGSLVTPERLRFDFSHFEAVTPEQIAAIEDLANDEILTNAEARHYEATMEE
                     ARAKGAIAFFGDKYGEVVRILEAGPKSIELCGGTHVSRLGDIGPVKIVSETSIGSNLR
                     RIEAVAGTGPVHRLREREAELADVAARAGVPVGEVVDGVGRLREQLKAAQDEVKALKK
                     QLAGAAAGNLAESAVDGIVVARVDGVERNDLRELALSLRDSPGVRAVVIGGAPEGGGA
                     ALVAATTEESGIDAGSLVADATRAIKGGGGKDPRLVVAGGKDPEGLDEALDLVRAAAG
                     IA"
BASE COUNT         1762 a         4663 c         5175 g         1907 t
ORIGIN      
        1 cgagggcagc agcgcctcgg tcatccgctc ccgctgcatg cgcagcaggt acaccgcgtc
       61 gaccgttggc aggacggcgt cgatgtcgtg gctgacccgc acggggtagg cgtcgatggc
      121 cggcggcagc agggtgggcg gagcgaccac ggtgacggtg gccccgagct tggtgaaggc
      181 ccagatgtcg gaccgggcca cccggctgtg cttgacgtcg ccgacgatgg cgatgtgcat
      241 gccgtcgagc gagccccgct gctggcggat cgtgtagcag tcgaggagcg cctgggtggg
      301 gtgctcgtgc cagccgtcgc cggcattgac gaccgaggcg tccacccagc gggtgagctg
      361 ccagggcacg ccggaggact tgtggcgcac gacgacgcag tcgatgccca tggcctcgat
      421 ggtctgggcg gtgtcgcgca gggactcgcc cttcttcacc gacgaggtcg acaccgagaa
      481 gttcatggtg tcggccgaca ggcgcttggc ggccgtctcg aagctgaggc gggtgcgggt
      541 cgagtcctcg tagaagaggc tgaccacggt cttgcctcgg agcgccggca ccttggggat
      601 cgggcgggtg gagacctcgg cgaagtgatc ggtgaggtcg aggaggcgct cgatgtcgcc
      661 ccgctcgagg tcgctgatgg agagcaggtc ggtcacttca cgacctcccc gagatcgacg
      721 ccctcgtcgt gcacgtcgac ggcctcgtcc ttgcgggtgg gcaggttctt gcccacgtag
      781 tcgggccgga tgggcagctc gcggtggccg cggtcgacca tgaccgccag ctggatggcc
      841 cgcgggcgtc cgaaggaggt gatggcgtcg agcgcggcgc ggatggtgcg gccggtgaag
      901 agcacgtcgt cgacgaggac cacgaccacg ccgtcgaggt ggaatggcac gtcggtgacc
      961 gcctcgggca gcaccgggcg caggccgatg tcgtcgcggt ggaaggccac gtcgagcttg
     1021 ccgaccggga cctcgacgcc ctcgatctcg agcagcgcct cctggaggcg ctcggcgagc
     1081 ggcacgccgc cggtctgcag cccgatgagc acgacctcgt cgacgccgcc gttggcctcg
     1141 atgatctcgt gggccatgcg ccaggtggcc cgacgcaggt cgtctgcgtc catcacctgg
     1201 gtccgggcaa cgaaaacgcc cccgaagggg cgtgtgagac gtcgttcgcg ttccgccatg
     1261 cggcgttcct tcctgtccgt ccgggcctca cgggacccgc ttcacggtca ggaggtcaac
     1321 gtagcaccgc cgtcgtggcc gaccagatcg tcttcccgtc cgatgcgccg agccggtccc
     1381 acccgagccg gtcgaacagg gcctcggcgg cgaggttggc cggatcggtg cgggcccaga
     1441 gctggtcgac ggccaggccg tcggggtcga gtgcccacgc cgtgaccgcc tcgaccgcac
     1501 ggcgggcggc cccggtgcgg cggtggggcg cggcgaccca cacgccgagc tcggcgcggg
     1561 ccgggtcggt ggtgaagccc gacaggccca cctcgccggc gacgtcgggg acgatcacca
     1621 ggtcgagggc gaggcgatgc tcccagcgct gctcggcgcc ggcgatccag gcgtcggcgt
     1681 cgaggcggtc gggcggcggg ttccagcggg cgatgtccgg gtcggcccag gcggcctcga
     1741 gggccggggc gtcctcagga cgccagtggc ggaggccctc aggcggggtc gggagcaccg
     1801 ggcacctgca ggcgccggag gacggcggcg atgcccgccg cgctgcgcat ctgcacggcg
     1861 tgccagccgg cctcgagggc gccgaccacg ttctcgtggc ggtcgtcgat gaacagcagc
     1921 cgctcggggg caacgccgag cacctcgtcg gccacgtgct cgaacgaggc gacgtcgggc
     1981 ttcatgagcc cgatgagcga cgagttgcag atgtcgtgga acagctcgtg gatgcccatg
     2041 gtgcccatgt cgacctcgag cttggtggtg gcgttggaga acacggccac ccgctggccg
     2101 gcgtcgcgca gggcggacac gatggcgacg acctcgtcgt cgatgcgcca ctcggtctcg
     2161 agccacatct gccgaccggc gtcggcgtcg acgccgtcgt gggcctcggc caggcgccgg
     2221 ccgatctcgg cggcccactc ctcggcggtg aggcggccgc acatggcgtc gctgtagagg
     2281 ggttcggaga aggcgatgga cccgaccgcg tccggcggga ggtcgtgggc gtcctggaac
     2341 cgaccgatgg cctgcgccgt ccagtgccgg atgacgccgt ccatgtcgaa gacgacgacg
     2401 tcgatcggca tccacgggcg gtcgccgtcg cggacctcct cggccacgcg ggccaggatg
     2461 ccgttcacga acttgggggt gtcgtcggtg ctgccgtagc ggccgcccag ctccacggcc
     2521 tcggacaggc acacgcccgg ggggacctcg agctgggtgg ccagctcgag cacgccgagg
     2581 cgcagcacca tgcggtccat ggcggccatg cgcttcaccg gccagcggtt ggcgtagcgg
     2641 tcgagcacgt ggtcgatctc gacctggtgg tcggcgacgc cgtaggccag ctcgagcgcg
     2701 tacgcgtcgg gccggatggg caggctggcg acgacgtcct cggggtgcag ggacttggtg
     2761 tcggcctcgt agagcagctc gacggcgcgc tcgcgcgcct cccggcgggt gctgggcagg
     2821 ctcacgctcg ggactggtac tggccggtgc gggtgtcgat cttgagcttc tcacccgtgt
     2881 tgatgaacag cgggacctgc acgacgtagc cggtctcgag ggtggcgggc ttggtgccac
     2941 cggtgctgcg gtcgccctgc agacccggct cggtctcggc cacggtcagc acgaccgacg
     3001 gaggcagctc caccccgacg atctcgtcgc cgtacatcat gagctcggcc gtggcctgct
     3061 cgaccaggta gttggtggcg tcgccgaggt tgctcggggc gacctcgatc tggtcgtagg
     3121 tctcctggtc catgaagacg tagctcgacc cgtcgttgta gaggtactgg tactcggagc
     3181 ggtcgatctt ggcctgctcg accttctccc ccgcccggaa ggtcttgtcg atgttggcgc
     3241 cggtgcgcac gttcttcagc gtggtgcgca cgaaggcgcc gcccttgccc ggcttgacgt
     3301 gctggaagtc ctgcacctgc acgagaccct ccggcaggtc gaggcacatg ccgttcttga
     3361 ggtcgttcgt ggagacggtc gccatggcga catggtgtgc cgtcggggac ggacctgcca
     3421 aatggtccgc tcgcggctca gggctccggg tcgtagggcg aggccgtgag gggtcggcag
     3481 ccggtggcgg tgaccaccac ggagtcctcg atgcggaccc cgccgtgttc gggcaggtag
     3541 acgcccggtt cgaccgtgac gcagtggccg ggggccagca gcgcggtgga ctcggggctc
     3601 aggcggggct cctcgtggat gacgaggccg atgccgtgcc cggtgccgtg gacgaaggcg
     3661 tcggaccacc cggcctcgtc gatcacggcg cggcaggcgc cgtcgatgcc gaccgcgggc
     3721 gccccggcgc ggacggcggc gaccccggcc gcctgggacg cacgcacgac ctcgagcatg
     3781 cgagcctgcg tggccgaggg ctcccccacc acgaaggtcc gcgtcatgtc cgagtggtag
     3841 ccgtcgcaga gcgcgccgaa gtcgaggacg accagctcgc cctcgcccgt ggcgccgatg
     3901 acccggtcgg aggggcgggc gtggggtcgg gcgccgttcg ggccgctggc gacgatcgtc
     3961 tcgaacgacg ggccctgggc gccgtggtcg agcatggccg actccagcgc gcgggcgaag
     4021 tcgcgctcgg tgggctgctc acgcagcagg tggcggacgt cggcgagggc ggcgtcagcg
     4081 atctcggcag cgcgctggag gcggtcgacc tcgccgtcgt ccttgacggc gcgcaggccg
     4141 agcagcaggc ccacggtggg gatgagcgag ccggggagcg cctcggccca ccgctgctgg
     4201 gtgtcccacg tgacgtcgtc ggcctcgagc ccgaccggtc cgatgccgtc gagcacggtc
     4261 gccaggcgat cggtggtgtc ccgggtgatc tcgatctcgg cggtgacgcc ggcggcggcg
     4321 agctgatcgg cggcctgctc ctggtagcgg ccgtcggtga ccagcacgag gtcgtgcggg
     4381 cggacgacga ggaccccggc cgagccggtg aagccggtga gccagcgcac gttcgtgagg
     4441 tcggtgacga cgagggcctc gcagccggcg tcgtcgagcg cggcccggac gcggtcggcc
     4501 cggggcgcga cctcgagcgg cgccaggtcg gttgggctca ccgcacgtcg cccagcgcgg
     4561 cgcgggcgac ctcggcgtcg acgcccgtga cgatctcgac gccgtcgtcg ccgtcgagcg
     4621 cgaacgtgag gccgtcgagg gccttcttgt cccgggccat gagcgagagg agctggtcgt
     4681 cgtcgaggcc ctcgggcacg cggtcgggca gctcgtagcg ggacacgatc tcgtggtgca
     4741 gggcgacgcg ctcgtcggag atccggccga ggcgccgggc gagggcggcg gcgtagagca
     4801 gtccgacggc gacggcctca ccgtggcgca ggtcgtactc gccggcggtc tcgagggcgt
     4861 gggccagggt gtggccgtag ttcaggatcg cccgcagccc gccctcgcgc tcgtcggcgg
     4921 cgacgatcgc cgccttgatg cggatgcact gggcgacctg ctcctcgagc tccatgtcgt
     4981 cgaggccctc cccgccgggg aggaagcggt acttggccag ctcgccgtac ccgccccgca
     5041 gctcccgcgg cggcagggtg tcgagggcct cgaggtcgca gaccacggcc gagggctgcc
     5101 agaacgctcc gaccaggttc ttgccctccg gcaggttgac gccggtcttg ccgccgatgg
     5161 ccgcgtcgac catcccgagc agggtggtgg ccacgtgcac gaccggcacg ccccggtggt
     5221 acgaggcggc ggcgaagccg gcgacgtcgg tgaccatgcc gccgccgatg gccaccacgc
     5281 agtcggcccg ggtcaggccc caggtggccc actggcggca caggtactcg accgtgcgca
     5341 tcgtcttggc gtcctcgccg gcctcgatgt ggaagacgcg gtgctcgacc ccgggatcgg
     5401 cggtcatgcc gatgccgcgc tgggtcacga cggccacccg gccggtgccc tcgggcagca
     5461 gggcggccag ctcccgccgt gcgccgcgcc cgacgacggc cgggtacgaa cggcccggga
     5521 cggggacctc gacggtgatc accggcgcct caccgggtct ggacgtaggc gtcgtggttg
     5581 cggaccagct cgtccaggga gtcgccgccg aacttccgcg tcgcctcggt ggccagcacc
     5641 agggcgacca tcgtctcggc gaccacgccc atggccggca cggcggtgac gtcggtgcgc
     5701 tccttgaagc tcaccgtctc ctccttggtg cgcacgtcca cggtcttgag ggaaggccgg
     5761 ttcaaggagg cgagcggctt catcgcggcg cgggccacga gcagctcgcc ggtggtgata
     5821 ccgccttcga cgccgccggc ccgggtgccc tcgcggacgt agtcacctcg cccgtcgcgg
     5881 gcgtcgggcg cccagccgat ggggtcgtgg gcgtcgctgc cccgcaggcc ggcgacctcg
     5941 aagccggcgc cgatctcgac gcccttgatg gcctggatgc tcatgagggc ctgggccagg
     6001 gccgcgtcga tcttgcggtc ccagtgcacg tggctgccga ggccgaccgg gacgccgtag
     6061 gcgaggacct cggcgacgcc gccgaggctg tcgccgtcct tggcggccgc cttgatctcc
     6121 tcgaccatgg cggcgctggc ggtgggatcg aagcagcgga cctcgtcctc gtcgacggcc
     6181 cccaggtcgc caggtgcggg ccggatgccg gcgctcgccc gggcctggcc catctggatg
     6241 acgtggctga gcacggagat gccgaggtgc gagagcagcg ctttggccag cgtgccggcc
     6301 gcgacccggg ccgccgtctc gcgggccgag gcccgctcga ggacgtcgcg cgcgtcgtcg
     6361 aagccgtact tctgcatgcc ggcgaggtcg gcgtggcccg gccggggctg cgtggccggg
     6421 ttcttggtcg cccccggcgc cggcgacatc tcctcgtgcc acttgtcgct gcgggtccac
     6481 tcggtgttgg cgatctcgat cgcgacgggc gaaccgaggg tgcggccgtg gcggacgccg
     6541 ccgaccaagg tgacctcgtc ctgctcgaag cgcatgcggg ggccgcgccc gtagccgagc
     6601 cggcgacggg ccagctcgcc ctgcacctcc tcgacctgga tcggcaggcc ggcggggagc
     6661 ccctcgacga tcacgacgag ggcggccccg tgcgattcac cggcggtcag gtagcgcagc
     6721 acggccggga gcgtagaggc ggtgccggcc agggcacgaa gcacatgggg cggggttccc
     6781 tgtcccgggt cagagcggcg tgatgcccca cacgacggcg atcgtgcagc cggcggccag
     6841 cgacgggccg tacgggaaca ccaccttggt cgagcgccgg gcgatcgcgt acaccccgcc
     6901 gctgagcacg ccgaggatcg agccgagggc tgcgcccagg aggacggcgc gcagcggtcc
     6961 gagcaggagg tcgagcgcgc cacccggttc gtgcccccgc caccccaggt agaagcccat
     7021 gagccaggcc agcttcacgt cgccccaccc catgccccgg ggcgagatca ggtggaagac
     7081 gaacagcatc gaggcgtaga ggacgccgcc gatgagggcc ccgacgatgg tgcccggcgc
     7141 cccgtcgatg agcgaggcga ggacgatggc accgaggccg atccccatcg aggggaagtt
     7201 gatgcggtcc gggatgcggt acacccgcag gtcgacggcg gcgagggtca ccagggcggc
     7261 gaccaggaac aagatggccg ccgtcggcgc cgaccaaccg agctcggcaa ccgtggcggc
     7321 gaacgtggcg gccatgatca cctcggtgac cgggaaccag cgccgccacc acgtccccgg
     7381 gcgggcgatc gccagcacgg gcacgggcgc caggtcggtg ggcacgggct ccagctcgtc
     7441 gagcagggac tcgtctgcac cttccgggag gtccttcgga tcggtgacga cgtagcccgg
     7501 ctcgcggacg atcagccggt tggcgaggga cccgacgacg aggccggcga tcccggcgag
     7561 caccacggcg acgacgtcca cggccgggca ccctaggttc ggccggagcc ggcgacggtg
     7621 acgccgccgg ccgagcgtgg atcagttctt ggtggaggac aggaagcgca gcagcaggtt
     7681 ctggtcgatc tcgtcgctgc cggatgccgg ctcgtccgca ccgacaggct cgacgggctc
     7741 gacctcggcc accggctcga tcgtctcgac cggctcgtcg gcggcgggct cgtctgctgc
     7801 ggcctgctcg tctccgcccc aggtcgcctc gacggccgcg gccgccttcg ggctcagctg
     7861 ggacagctgg gaggcgaagt cgtcgtcgtc ctcgggctcg gtgtccggct cgaagctctc
     7921 cgcctcggcg gtgtccggct cgaacgtggt ctcggcctcg gaggtctcgg gttcgtacgt
     7981 ggcctcgggc tcgacgaccg gttcctcgac ggtcacgggc gctgcgacgg tgtagggctc
     8041 ctcgaccgtg ccgaccgcca cgaaggacgc gaggtcggtg ccgagggagc tgaccgcatc
     8101 ggggtccagg gcgggctcca cgaacgtggg ctcctccacc gtggcgaacg tggtcaccgg
     8161 ggcttccacg acggcctcgg tcggtgcctc ggtgagctcg accagcccgc cctcgaccag
     8221 gtcgcggagc tggcggcaca cgtcgtactc gccctgctcc aggcgctcgg ccagggcgtg
     8281 cccgcttgca ccggtcccga cctgggcgag cacccgccac tcggtcggtg cgatggtgcg
     8341 ctcgtcgtcg agctcggcga ccatgtgcac ccacacgtcg agcgacggca ccacggtctc
     8401 gatctcgcgc cactcgacca gccgggcctc ggcctcggac agcacctcgt ccacggtccg
     8461 gggctcgcgc tcggccgggg gctccgctcc ggagtcgaag tcgaagcggg catcgacgaa
     8521 gcgcagcagc tcgaaggcca cgccttcgac tgcgtcctcg acagcacggt cggcatcggc
     8581 agcgacgatc gcaccgtcgc gcacccagac ccgaccgcga ccgcggtccc cgtccagggc
     8641 cagcaggccg gtcttcttcg tgctcgagag gagccggagg acatccggca ggctgaacgt
     8701 ctcgagggtc ccggacagcg ccacgacgat ctccttcaga ggctgggctt cgctccccgt
     8761 tccttcggcg cgatcgggcc cgtcctgtag ggggaatccg ctcgaggttc cctcagccgg
     8821 ctgctgcggc ccgcatgacg tcgaccgggg cgtcgcggcc ggtccaggcg tgcagctggc
     8881 gcgccgcctg gtggacgagc atcccgaggc cgtccacggt ctgcaggccg cgctcctggg
     8941 ccgcggccag caggggtgtc accagcgggt ggtacacgag gtcggcgacc acggcaccgg
     9001 cgggcagcag gtccggatcg gcggccaggg tggcgtcgtc gcccatcccc accggggtcg
     9061 cctggacgag caggtcggcc gtgccgaggt cggaggtggt gccgacggcg ccgacgtccc
     9121 cggccagggc cgcggccgcc tcgcctcgac cgggatcccg gttgagcacg gtgacctgcg
     9181 ccgcgccggc gacggccagg gcccgcacga cggctcgggc tgcaccgccg gcgccgagca
     9241 ccacgcagcg gcgtccggcc ggatcgaccc cggcctcggc cagcgagtcg acgaagccgc
     9301 ccccgtcggt gttgtcgccg atcagccgat cgccctcccg gcgcacgctg ttgaccgcac
     9361 ccaacgctgc ggcgtcggcg gtgaggacgt cgacctcggc ggcgaccgct cccttgtgcg
     9421 gcatcgtcac cgacagcccc tcgaggccga gcgcccgcat cccggcgagg gcggcgggca
     9481 cctggcccgg ggcgacgggg agggcgacgt acacccagtc gagcccgagg gcctcgtagg
     9541 cggcgttgtg gatggcgggt gaccgggagt ggcgcaccgg ccaaccgatg acgccggcca
     9601 ggtgcgtggc cccggtgggg gtccggatca cccgcagccg aggccgagtc gggcgcactc
     9661 ggccttggcc tggttgaact cgctgaacga ctcggtgaag aagtggttcc cctgctcgtc
     9721 ctggagcacg tagtagatcc acgggccgtc ggcggggttg agcgccccct cgagcgacgc
     9781 ctggcccggt gcagcgatgg gcgtcggcgg gatgccggcg acccggcggg tgttgtacgg
     9841 ggagtcggac tcgaagtcga gcgagtcacg gccacgtccg gcgagcaccg actcgtaccg
     9901 gctggtggcg tcgatgccga gggggatgcc gtcagccagc cggttgtaga cgacccgggc
     9961 gaccttggcc cgctcctcgg ggacccgggt ctcagcctcg atgagcgagg cgatcaccag
    10021 gacctcgtag ggggtgcggc cgagctcggc ggcccgcgtc tcgatgtcga gcccggcgag
    10081 ccgggcgtcc atctcggcga cgaggcggcc caggaaggcg gcctcgtcca cgtcctcgtc
    10141 gatctcgtag gtgtccggga agaggagccc ctcgagcgcg caccacggcg gaccaccctc
    10201 ctggatgcac gcgctctccc cctccggctg gaactggctg cggatctccc ccgaagacgc
    10261 tgcggcttgc agggcctcga gcgaccagcg ctcgacgccg cgctcggggt cggcaaggcg
    10321 gtccaggatc tcggtcacgg tgaggccctc ggggatggtg acccgttcga gctgcggtgg
    10381 tcggggcccc tcgtcgagca cggcgacggc ctcgtcgaag gacgagttct cctggaactc
    10441 gtagaagccg gcctggaacg gcccgacgct ctgcaggcgg gtccagtagt tccagatcgt
    10501 cgacgacgtg atgatgtcgt tgtcgaccag cagggcgccg atgtcctcgg tggtggagcc
    10561 cgaggggatc tcgagctcca cggtggcgcc gggcgggccg ggcgggtcga tgcggtcctg
    10621 gacctggttc caggccacga acgcgccggc ggccagcagg gccagcagca cgaggaaggc
    10681 gatgaacagc ttgcgcccgg tgtgggacgg cgggtgccag tcgtcccact ccccctcggc
    10741 ccagggctcc tcctccacgt agtcgccctc gtggtcgtgc accgtggtgg tgtcccagtc
    10801 gtcggcggga tcgtggtccc tggtccagtc gtcgttcacg acggctcctc tgttggatgc
    10861 tcggcaggtc gggcgtcgag ccacgactgc aggatgacgg cggcggcgac cttgtcgacg
    10921 acgcggcgcc gggccgggcc gcggagctgc tgctcggtca acgaccgctc ggcggtgacg
    10981 gtggacaggc gctcgtccca ggtctcgatc ggcacgtcga gcgcctcggc gagctggtcg
    11041 cactcggcga ggacccgctc ggcggcgtgg ccgatcgagc cgtcgaggga caggggcagg
    11101 ccgacgacca cgacctcggc ctcggtctcg gcgacgtgct cggcgatggc gcggtggtcg
    11161 cgccggcggt cgccggcgcg cgccagcacc tcgtagggcg tggccagggt gcccgaggac
    11221 agggcgatgc cgatccgctt ggatccgagg tcgagggcca gggcgcgcac cccggtctca
    11281 ggcgatgccg gccgcggcgc ggaccaggtc gagggcctcg tcgagtccct cggggtcctt
    11341 gccgccggcg acgaccaggc gtgggtcctt gccaccaccg cccttgatgg cccgggtggc
    11401 gtcggccacc aggctgcccg cgtcgatgcc ggactcctcg gtggtggccg cgaccagggc
    11461 cgcgccgcca ccttccggag caccgccgat gacgacggct cgcaccccgg ggctgtcgcg
    11521 gaggctcagc gcgagctccc gcaggtcgtt gcgctcgacg ccgtcgaccc gggccacgac
    11581 gatcccgtcg accgccgact cggcgaggtt gccggcggcg gcgccggcca gctgcttctt
    11641 gagcgccttg acctcgtcct gggccgcctt cagctgctcg cgcaggcgcc cgacaccgtc
    11701 gacgacctcg ccgacgggca cccccgcccg ggcggcgacg tcggccagct cggcctcgcg
    11761 ctcgcgcagg cggtggaccg gtccggtgcc ggcgacggcc tcgatgcggc gcaggttcga
    11821 gccgatggag gtctcgctga cgatcttcac cgggccgatg tcgccgaggc gggacacgtg
    11881 ggtgccgccg cacagctcga tcgacttggg gccggcctcg aggatgcgca cgacctcgcc
    11941 gtacttgtcg ccgaagaagg caatggcgcc ctttgcccgc gcctcctcca tggtcgcctc
    12001 gtagtggcgg gcctcggcgt tggtgaggat ctcgtcgttg gcgaggtcct cgatggcggc
    12061 gatctgctcg ggcgtgaccg cctcgaagtg gctgaagtcg aagcgcagcc gctcgggggt
    12121 gacgagggag ccctgctgct tgacgtggtc gccgagcacc tcgcgcagcg cccagtgcag
    12181 gacgtgggtg ccggtgtggt gccggcggat ggcgttgcgc cggtcgctgt cgatgctggc
    12241 ggtgacctcg tcgccgaggt cgaaggcacc ctcggtgatc cgggcgatgt gccggtgcag
    12301 gccgggcagc gcgtagacgg tgtcgagcac ctcggcccgg gcgccgtcgg cggtgatctc
    12361 gccggtgtcg cccacctggc caccggcctc ggcgtagaag ggcgtggcgg cgaggaagat
    12421 cgagacggtg tcgtcggcac cggggaccac gccgacgacc gtggtcgtgg tgctgtcgac
    12481 ctcgcgaccg acgaactcgg tgggtccgct ggcgtcgagc acggagcgga aggcgtcgac
    12541 ctcgtcgccg acggccacgc cgccgccctt ggacgccgcc ttggcccgag cccgctggtc
    12601 ggccatggcc ttgcggaagg cgtcctcgtc cacgtcgtag ccacgctcgg ccgcgatctc
    12661 ctgggtgacc tcgaacggga acccgtgcgt gtcgtgcagg gtgaaggcga cggtgccgtc
    12721 gaccgtgccg ccctcgggga tggtggcgat gtgggtgtcg agcatcgtcg agccggtgcg
    12781 cagggtccgc cggaagcggt cctcctcgcg gctgacgatg gtgagcacgc tgtcggcgct
    12841 ctcggcgagc tcggggtagg actcccccat cagctcgacg caggtctcga tcatcggggg
    12901 cagcaccggc ttctcgacgc cgagcatgta ggagaagtac acggcccggc gcaggacgcg
    12961 gcgcagcacg tagccgcggt cctcgttgga gggcatgacg ccgtcggcga ccaggaaggc
    13021 cacggtgcgg gtgtggtcgg cgatgaggcg cagggcgatg tcgtggaggc ggtcgccgcc
    13081 gaagcgggcg ccggtgacct cctgggcccg atcggtgagc accgagaggg tgtcggccgc
    13141 gtagagcgac ggcgaatcgg ccaggacgcc gaggatccgc tcgagccccg caccggtgtc
    13201 gatgccgggg gcgtcgaggt cgcgcagcga gccgtcgggc tggcggaagt actgggggaa
    13261 caccaggttc cagaactcga cgtagcgctc ctcggccgcc gggttggccg gaccgccgtc
    13321 ggggccctcg tccggaccga agtcccagaa gagctccgag gacgggccgc aggggccggt
    13381 gtcgcccatc tcccagaagt tgtccttgtc gaggcgctgg atgcgctcgc gcgggaagcc
    13441 gacggcgtcg gcccagatgt cctcggcctc gtcgtcggag gtgtggacgg tgatccagat
    13501 gcggtcg
//
LOCUS       DATISU010000364        16939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_261516, whole genome shotgun sequence.
ACCESSION   DATISU010000364 DATISU010000000
VERSION     DATISU010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 16939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16939
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261516"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VLR27_14465"
     CDS             complement(<1..274)
                     /locus_tag="VLR27_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSP04712.1"
                     /translation="MTRPRLLVGVCGTATEVGKTWASARLIEVLVGDGRTVAARKPLQ
                     SFDPDDDAPTDAEVLAAATGETPHDVCPEPGWYPVPMAPPMAAAALG"
     gene            complement(271..1422)
                     /locus_tag="VLR27_14470"
     CDS             complement(271..1422)
                     /locus_tag="VLR27_14470"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HSP04713.1"
                     /translation="MSRSHRSWVRAELDAIRGQDRWRRTRSFDALGPAGSLEDRPVVS
                     FASNDYLGLSAHPAVVAAGHDALDRWGAGATASRLVVGSRPVHHDLESALAAWKGTEA
                     AAVVPSGYAANLAVLTTFGGPDCLVVSDELNHASIIDGCRLSRSHVAVARHADVDHVD
                     DLLASAGSRRAIVVTDSVFSMDGDEAPVQALAECCARHGALLVLDDAHAVLGPDADLA
                     AVDGLDVLHVLTLSKALGSLGGAIAGSQDAVDLVVNRARPFIFTTALSPADAAAALAA
                     VEVVRSPEGDALVARLRAHVDRLRPGHPSPIIPVVLGEEQAALDAAEALLAQGLLVPA
                     IRPPTVAPGTSRLRIALSAAHTDDQISTLAAALHPFGTGSAPVAPAGVR"
     gene            complement(1419..2711)
                     /locus_tag="VLR27_14475"
     CDS             complement(1419..2711)
                     /locus_tag="VLR27_14475"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HSP04714.1"
                     /translation="MPPQDASAQDLADRDRARVWHPYAPMPGALAPVPVAGAHGTRLV
                     LADGREVVDGMSSWWASIHGYRHPVLDEAVRAQLDATAHVMFGGLTHAPAVELAELLV
                     SIAPEGLEHVFLADSGSVSVEVAMKMALQHARGTGRPGRTRLLTVRGGYHGDTLGAMS
                     VCDPVNGMHSMFTDVLPTQLFAPAPTPAFGEPFDESHVSELAALLSHHRDEVAAVILE
                     PVVQGAGGMRFYAPDYLAAVRRLCDEHDVLLIADEIATGFGRSGRLFGCDHAGITPDI
                     MCLGKALTGGYLTMAAVLCTPAVAAGVSAAESGALMHGPTFMGNPLAAAVSLASVRLL
                     LSRDWETEVAGIEAGLRAGLDDLRDHDAVADVRVLGAIGVVELHEPVDMAVSQETLLD
                     HGVWLRPFGRLLYTMPPFVSSEDDVAAITTAMRAVVVG"
     gene            2863..4359
                     /locus_tag="VLR27_14480"
     CDS             2863..4359
                     /locus_tag="VLR27_14480"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSP04715.1"
                     /translation="MLNVVTGRVLIVGGGVGGLTAALGLARRGFEVEVLERDPLPDVT
                     DAEAAFAAERRGAPQVHQTHGFLARLVVELRDRHPDVLDQLLDAGCVTMPTTTQLGDP
                     RPGDEDLRVLVVRRTTLEWVLRRAVADEPGVTMHTGVGVQGLLGTSAGDGRPAVVTGV
                     VTDDGQERHADIVVAANGRRSPVGRWLDELGVAVPETVHESGLMYLSRWYRFADDAEI
                     VLDPKLGGDLGFVKYLAVPGDGGTLSITLAIRTEDAELRAALSDADAFEHACRILPGP
                     DRFVGRYDLTPLGGVRPMTGLLNRIRRFVDDDGNPTVLGFHALGDSHTCTNPLYGRGC
                     ALAAVQANRLVDAATAHPDDPSARAIAYEQTLAREVEPWFDISVQMDRMGADPAGLAI
                     GGGAGSDGGGAKAMGALFAAAATDPVIGRGMARFWNLLALPADLMTDPELMARMAEVM
                     ADPDAWPMPPVEGPNRTELLDALAALPTDPNEDPVPTTSDRTTDRAAS"
     gene            4356..5351
                     /locus_tag="VLR27_14485"
     CDS             4356..5351
                     /locus_tag="VLR27_14485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP04716.1"
                     /translation="MSAPLPAGVTERTIRVGDIDMHLLEAGTEGPLVVLAHGFPELAW
                     SWRHQLPALADAGYRVVAPDQRGYGGTSRPEAIEDYDIHHLTGDLVGLLDELGEERAI
                     FIGHDWGSMVVWQLSLLHPDRVMGVCGMSVPFVPRASAPPIQIFRAIFADQFFYIVYF
                     QEPGVADAELGGDPARTMRRLLAGLSTPGDLTTDEAASMSADDGRGFVDRMPEPDGLP
                     DWLTQEELDHYVEVFSETGFTGGINWYRNFDRNWELTPQLAGAKVEVPSFFVGGADDP
                     VLTMSPPAGQEAWLTDHRGSVIIDGAGHWVQQEAPEQVNAALLDFCDQLRGGTNL"
     gene            5348..6550
                     /locus_tag="VLR27_14490"
     CDS             5348..6550
                     /locus_tag="VLR27_14490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSP04717.1"
                     /translation="MSTAPSRLKFGAFYAPFHPPGQNPTLALEYDLQRAEALDRFGYD
                     EVWFGEHHSGGYEIIPSPEVFIAAAAQRTKHIKLGTGVVSLPYHHPLMVADRMVLLDH
                     LTRGRVIFGTGPGALPTDAYMMGIDPVEQRRMMEESLEAILALLRTDEPVTRETDWFT
                     LRDAKLQYRPYSDPHFEVSVAAMVSPSGPRLAGTHDVSLLSLSMSIADGFAAIGQAWN
                     VVEDMAAKAGHKEPDRANWRVLGTMHIAETRDQAIEDCTYGLRDFANYFGGGAGFVPL
                     SNEVTEGTTSERDFVEAYVEGGKGNVVIGTPDDAIEYIEGLLEQSGGFGTFLMLGHDW
                     ARPDRTIESYRLFAERVMPHFQGQLQAQRDSHDWATGKRNELFGRAGQAIMNAVQQHV
                     QEQDAEQA"
     gene            6550..7596
                     /locus_tag="VLR27_14495"
     CDS             6550..7596
                     /locus_tag="VLR27_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSP04718.1"
                     /translation="MRAAVLNKGEILVRDDVEEPTPGFGQVLVEVKACGICGSDLHFA
                     KHGGDMLALGKQIEGMPSMGGEGDGVDLDRDVYMGHEFAAEVLDVGPDTEAAAPVGSL
                     VTSMPVLLGMDGIHPIVYSNTVKGGYGERMLLSAPMLLEVPNGLSAEHAALTEPMAVG
                     LHAVNKARLEQGEGAIVLGCGPVGLAVIAALAVRGVGPIVAADFSPARRRLAEQMGAT
                     DIVDPREETGWQAWTRAGNGRAPVVFEAIGVPGILDDILRCAPAQSRVVVVGVCMGHD
                     TINPFFGISKELNIQFCLGYDPMEFAGSLRSIAEGEIDVAPMITAQVDLDGVPQAFAD
                     LGDPDEHCKILVVP"
     gene            7593..8768
                     /locus_tag="VLR27_14500"
     CDS             7593..8768
                     /locus_tag="VLR27_14500"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSP04719.1"
                     /translation="MTKPMHETHRIAYDGAVDADGHILEPPDLWETYIDPQFRDRALR
                     IRTDEQGLEELEIGGERSQMARRGMPSTLGAMGAPDLAAMQKDPERTYLREAPYGSMD
                     PAQRLEVLDAENLDAAILYTTVGLLWEAELTDPELSQAYTKAYNRWICEFCADSPRLV
                     PTAHLSLSDPVAAAAELERAIGEGAKGCYVAPFTHDAKPLGHPDNDPVFAVAQEAGIP
                     FAIHPTFEPQWTKGQRMGTWENVKQLRLLASVTASDGVRQQFTTLFDYGVFDRFPELK
                     VLVLESGGGWIGYWLDRIDAVYGHTFIGERVPLQEKPSDYFRRQCWISCDPDERTIPA
                     LADRFGVDRFLWASDFPHADHTPEYVEDLNELAGMFSDADRRKFLGDNARALFDIDA"
     gene            8840..9433
                     /locus_tag="VLR27_14505"
     CDS             8840..9433
                     /locus_tag="VLR27_14505"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP04720.1"
                     /translation="MAGAAGARKRVRMDHEERRKQILDAATALFLRRPYSQVSVSDIA
                     EAAGVAKGLLHHYFDSKRDLYLEVVRDVSSVPLPVGAPAGPGEDPAWAAAIDALLAFV
                     SETRELWLRATTVGDDEVASIVDDSREVLAEQTIEALGLDDTPVVRALVRGYGGLVQE
                     VTREWLDRGRLTEAQAREVLVRTLPLLLERVLPDLRP"
     gene            complement(9423..9809)
                     /locus_tag="VLR27_14510"
     CDS             complement(9423..9809)
                     /locus_tag="VLR27_14510"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HSP04721.1"
                     /translation="MAHDFLSDEWMDAIEALRPEAPEPPAATKDLVINLVVPDTPFGE
                     REAHMAGGQFERGLHDSAPTKLTVPYEVAKSVFIAGDQQAAMQAFMSGQIKIDGDMTK
                     LMAMQSAGAPSPEQVAFQEKIKALTA"
     gene            10079..11479
                     /locus_tag="VLR27_14515"
     CDS             10079..11479
                     /locus_tag="VLR27_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04722.1"
                     /translation="MTAAASVTMLGLSVLLAPGGVAQEVPQSAPESGEIRNGTAKAVA
                     TVAKIAPGVGQLELGITMGTAVTQLTNDLAQATAQTADLGLIGSSLTSEGCDGDASFT
                     PDQLPQPTSVDNRKGDASQSRDEGGTDGAPVALGRMQVEATEDAPASRAVASSTILGS
                     PDLLRIGGGDAESSTRVLPGEGREATATVTSSLSITDAVRLDGMRWHATHRTGVDPLA
                     EGAFELGRAEVGGVPLPTEDLASAEAAINEALAPTGISISLPRVQRMLEPTDLIRVTP
                     MRIEIRDSPLGKTALGPALDATRDQRVQLFDDLVELYCDSASVLLVGDIGISVVAGTG
                     FLTVDLGGVQASSSDLEVGNPFGTIEPFELGGVSIPGTGVDTSTAAPPAGSPPVAGVS
                     PPSTVAAAPAVGAVPARATGPVEELCESLHPNRGTCSEGAAAAVGVVGLLATVGMAGA
                     DVLRQRRQAALAEDLA"
     gene            11476..13143
                     /locus_tag="VLR27_14520"
     CDS             11476..13143
                     /locus_tag="VLR27_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04723.1"
                     /translation="MIGSLLGHRGLRPYLPVGAAAVAFLVMASVVGPAPRSHQALAGS
                     VEVPLAAGPQQSGPGAGDAGAPGSTAPGADGTDPGGPGGATPGTATDPQSDGGGGVTP
                     CPDRAAQVPGDPYSPPCLVFSGDNGGATTTGVTADEIVVSVRRLEGPTAGEIFADISG
                     ESVEDSPESYANTLDALAQYFSERFQFYGRSLRIEIYDGEGVGSSELLGGGREAALAD
                     SVRAREIGAFADLSAITLPYADALTRQGIIGFGAPYPSRSWFEERRPYAWSLFPDGTL
                     VSEAGAAATAARLAGQQTAEYAGEDIKGRQRVYGLVAPENAEYQESVERYISAIGEHG
                     ITFAKSLRYKLDISSMPNQASNIIAQLKDAGVTTLVCACDPVMLALGMAPKANEQSWE
                     PEWLTGGLAFVEQDIVAQLIDSRQWRHAFGTAFNAESEPIGASYPYAAFKQIRPGDEP
                     AFGVEEIYYSMYLLALGIHLAGPNLTPESFEAGMFSYQGASGPRGYWSFGPGDYTPTD
                     DFREIWWDPDRISAQNNKPGAWVQVNGGQRWFADTIPTGPAPFFQEG"
     gene            13145..13339
                     /locus_tag="VLR27_14525"
     CDS             13145..13339
                     /locus_tag="VLR27_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04724.1"
                     /translation="MPTTTEALEVLRRRPAATELFGGFGPLVLAAALLLAMVLLAPSV
                     APERTVERPVEPGTSTEAGR"
     gene            13336..15465
                     /locus_tag="VLR27_14530"
     CDS             13336..15465
                     /locus_tag="VLR27_14530"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP04725.1"
                     /translation="MIARTAERLRTDRASQVVLATAVAYTLLYWVPLSPLPRLVPWGV
                     IAQGVIFGTSYALLAMGLILIYRTTRIVNFAYGAMGAMPGSLTVGLFVAKGWSYWVAI
                     LVGVAVGVATGAAVDVFVIRRFARSSRLVLTVASIGLAQILGAVGLIIGVALGTDALI
                     GNIDTPLSGSWFVRPYPIRGDHLLMLAVAPLTLAGLGWFLLGTDTGRAVRAAAENEDR
                     ALLLGVPVRRLQTVVWAIAGGVSTITFVTKAPFTGVVPDALVGATTILPGLAVAVIAR
                     FKSLPLALVGGIGLGIAEWTIRWNVRAESVFDVAFLVAILVALLLNRERTTRAESGES
                     SWDAAGVLKGIPRELRDVPEVRVPMRVLAVVGLAAVVLYPLSFAPSTMVTLTFGVLWC
                     MVGVSLAVLTGWGGSISLGQFAIVGVGAMATGNALIRWNLDVFASLALAVAAGAIVSV
                     LVGVPALRIKGLYLAVTTLAFAVALDSYFLNPVNFPELVPDRIIRPVLWKRFDLGSEW
                     TTYYLCLATLGATVLVVRAMRRRRAGRVMVGVRDNERGAAALAVPTTRVKLQTFLVAG
                     CIAGLAGGLYVVVAGGTGQGTFRPGMSIEVFSFAVIGGLGSVAGVISGVVMFRLLDFV
                     LARGVDGAAADILRLSLTGGGLLLILYFLPGGVWQYVQRARDRYLRVVADRRGILVPS
                     LVADRRVEDGAKDEPEDETSVISGALT"
     gene            15462..>16939
                     /locus_tag="VLR27_14535"
     CDS             15462..>16939
                     /locus_tag="VLR27_14535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSP04726.1"
                     /translation="MSEPLLRVEGVDAAYGPVQILFGLDVDVHEGEIVALLGTNGAGK
                     STLFKCITGLLPPTDGKILFKGEDLVGRTTDEIAELGVVMMPGGKSVFPTLTVRENLR
                     LASWLKRKDKQAVLDGEARVLELFPRLAERIDQLAGNLSGGEQQMLAVSQALIPDPEL
                     LLIDELSLGLAPTIVGQLIDVVRQVHASGLTVVVVEQSINVALTLAERAVFMEKGEFR
                     FTGSTRELLERPDILRSVFISGGTDSSGPDSETAAGTPGTVPATPVRTAPEPDAPAIL
                     ECHGVTKRFGGITAVDHVDLELRQGEILGLIGQNGAGKTTLFDCVSGFLPIDEGRILL
                     RGHDITSLAPHERARRGLGRSFQEARLFPTLTTAETLAVARERHVRGTSMVADAFGQP
                     VARATDDDIATAVDLLIELMGLGAFREKLTGELSTGTRRIVELACLLASDPKVIVLDE
                     PSGGVAQRETEALGPLLQRVQAATGTSVLVIEHDMPLLTSIC"
BASE COUNT         2319 a         6468 c         5852 g         2300 t
ORIGIN      
        1 ggcccagcgc cgcggccgcc atcggcggtg ccatcggtac ggggtaccaa cccggctccg
       61 ggcacacgtc gtgcggagtc tcgcccgtgg ccgccgccag cacctcggcg tcggtgggtg
      121 cgtcgtcgtc cgggtcgaac gactgcagcg gcttgcgggc cgccaccgtg cgaccgtcgc
      181 cgaccaggac ctcgatgaga cgggcgctcg cccaggtctt gccgacctcc gtggccgtcc
      241 cgcacacgcc gacgagcagc ctggggcggg tcatcgcaca cccgcgggcg ctacaggggc
      301 actgccagtt ccgaaggggt ggagggcggc ggccagcgtg ctgatctggt cgtcggtgtg
      361 cgcggcggac agtgcgatgc gcaggcggga ggtgccgggg gcgaccgtcg gcgggcggat
      421 ggccgggacg agcaggccct gggcgaggag cgcctcggca gcgtcgaggg cggcctgctc
      481 ctcaccgagc accacgggga tgatcgggga cgggtgacca ggacggagcc ggtcgacgtg
      541 cgcgcgcagg cgagccacca gggcatcgcc ctcggggctg cggaccacct ccaccgcggc
      601 gagcgcggcg gcggcatccg cgggcgacag agcggttgtg aagatgaacg ggcgggcccg
      661 gttcacgacc aggtcgacgg catcctgcga tccggcgatc gcaccaccga gcgagcccag
      721 cgccttcgac agggtgagca cgtggagcac gtcgaggccg tcgaccgcgg ccaggtcggc
      781 gtcgggcccg agcacggcgt gggcgtcgtc gagcacgagc agggcgccgt ggcgggcgca
      841 gcactcggcc agcgcctgca ccggggcctc gtcgccgtcc atggagaaca ccgagtcggt
      901 gaccacgatc gcccgccggg acccggccga cgccagcagg tcgtccacgt ggtcgacatc
      961 ggcatggcgg gccacggcga cgtgggagcg cgacagccgg cagccgtcga tgatcgaggc
     1021 gtggttcagc tcgtcgctga ccacgaggca gtcgggaccg ccgaaggtgg tgagcacggc
     1081 gaggttggcg gcgtacccgc tcggcacgac cgcagcggcc tcggtcccct tccacgcggc
     1141 cagcgccgac tcgaggtcgt ggtgcacggg acgggagccg accaccaacc ggctggccgt
     1201 cgcaccggca ccccaccggt cgagggcgtc gtggccggcg gccaccacgg cggggtgggc
     1261 ggacaggccg aggtagtcgt tcgacgcgaa cgacaccacc gggcgatcct ccagggaccc
     1321 ggcggggccg agtgcgtcga aggagcgggt gcgacgccag cgatcctgac cccggatggc
     1381 gtcgagctcg gcccgcaccc agctgcggtg cgacctgctc atccgaccac caccgcccgc
     1441 atcgcggtcg tgatcgcggc gacgtcgtcc tcgctgctga cgaagggcgg catcgtgtac
     1501 agcagtcgcc cgaacggccg cagccagacg ccgtggtcca gcagcgtctc ctgggagacg
     1561 gccatgtcga ccggctcgtg cagctccacg acgccgatgg cgccgagcac ccggacgtcg
     1621 gcgacggcgt cgtgatcccg caggtcgtcc agccccgcac ggagcccggc ctcgatgccg
     1681 gcgacctcgg tctcccagtc ccgggacagc agcagccgca cggatgcgag cgacacggcc
     1741 gcggcgagcg ggttccccat gaacgtcggt ccgtgcatca gcgcaccgga ctccgccgcg
     1801 ctcacgccgg cggccacggc cggcgtgcac agcacggcgg ccatcgtgag gtagccgccg
     1861 gtgagcgcct tgcccaggca catgatgtcc ggcgtgatgc cggcgtggtc gcagccgaac
     1921 aggcgacccg accggccgaa cccggtggcg atctcgtcgg cgatcagcag cacgtcgtgc
     1981 tcgtcgcaga ggcgccgcac cgcggccagg tagtcgggtg cgtagaagcg catgccgccc
     2041 gccccctgca cgacgggttc gaggatgacg gcggccacct cgtcccggtg gtgcgacagg
     2101 agggctgcca gctcggagac gtgggactcg tcgaagggct cgccgaacgc cggggtcggt
     2161 gccggggcga agagctgggt gggcaggacg tcggtgaaca tcgagtgcat cccgttcacc
     2221 gggtcgcaca cggacatggc cccgagggtg tcgccgtggt agccgccccg cacggtcagc
     2281 agccgcgtgc ggcccgggcg gcccgtgccc cgggcgtgct gcagcgccat cttcatggcg
     2341 acctccaccg acaccgatcc cgagtcggcc aggaacacgt gctccaaccc ctcgggggcg
     2401 atggacacca acagctcggc cagctccacc gccggcgcgt gcgtgagacc gccgaacatg
     2461 acgtgggcgg tggcatccag ctgggcgcgc accgcctcgt cgagcaccgg gtggcggtag
     2521 ccgtggatcg atgcccacca cgaggacatg ccgtcgacca cctcgcgccc gtcggccagc
     2581 accaggcgcg tgccgtgcgc cccggccacg ggcaccggtg ccagcgcccc cggcatgggg
     2641 gcgtaggggt gccagacccg ggcccggtcc cggtcggcga ggtcctgggc cgaggcgtcc
     2701 tgcgggggca tccgggcgaa cgtaccggcg ggcgtgccgc gcctccgagc cgccacccgc
     2761 ctccgtgacc gacgccacag cagacacccg gttgacggga tcgggaccgc cgcctacctt
     2821 gccctggcaa cgtgcgttgc cagggggcga ccgggtctgg gggtgttgaa cgtggtgacc
     2881 ggacgggtgc tgatcgtggg aggtggcgtc ggcgggctga ccgcggcgct gggcctggcc
     2941 cgtcgcggct tcgaggtcga ggtcctcgag cgggacccgc tccccgacgt caccgacgcc
     3001 gaggccgcct tcgccgccga gcgccggggt gcaccccagg tccaccagac ccacggcttc
     3061 ctcgcccgcc tcgtggtcga gctgcgggac cggcacccag acgtgctcga ccagctcctc
     3121 gacgccggtt gcgtcaccat gcccaccacc acccagctgg gcgatccccg accgggcgac
     3181 gaggacctgc gcgtcctcgt cgtgcgccgc acgaccctcg agtgggtgct gcgccgggcg
     3241 gtcgccgacg agccgggcgt caccatgcac accggcgtcg gggtccaggg cctcctcggc
     3301 acctccgccg gcgacggccg gcccgccgtg gtcaccggcg tcgtcaccga cgacggccag
     3361 gagcgccacg ccgacatcgt cgtcgccgcc aacggccgtc gcagcccggt gggtcgctgg
     3421 ctcgacgagc tcggcgtcgc ggtgccggag accgtccacg agagcgggct catgtacctg
     3481 tcccgctggt accgcttcgc cgacgacgcc gagatcgtgc tcgaccccaa gctcggcggc
     3541 gacctcggct tcgtgaagta cctggccgtg cccggcgacg ggggcacgct gtcgatcacg
     3601 ctcgccatcc gcaccgagga cgccgagctg cgggcggctc tctccgacgc cgacgccttc
     3661 gagcacgcct gccggatcct gcccggcccc gatcgcttcg tcgggcgcta cgacctgacg
     3721 cccctcgggg gcgtgcggcc catgaccggg ctgctcaacc gcatccggcg cttcgtcgac
     3781 gacgacggca accccacggt gctcggcttc cacgccctcg gcgacagcca cacctgcacc
     3841 aacccgctct acggacgggg ttgcgccctg gccgcggtcc aggccaaccg cctcgtcgac
     3901 gccgccaccg cccaccccga cgaccccagc gcccgcgcca tcgcctacga gcagaccctc
     3961 gcccgggagg tggagccctg gttcgacatc tcggtgcaga tggaccgcat gggcgcggac
     4021 cccgccggcc tcgccatcgg cgggggcgcc gggagcgacg gcggcggcgc caaggcgatg
     4081 ggcgccctct tcgccgcggc ggccaccgac ccggtgatcg ggcggggcat ggcccgcttc
     4141 tggaacctgc tcgccctgcc cgccgacctc atgaccgacc ccgagctgat ggcccgcatg
     4201 gccgaggtca tggccgaccc cgacgcctgg cccatgccgc cggtcgaggg accgaaccga
     4261 accgagctgc tcgacgcgct cgccgccctg cccaccgacc cgaacgagga tcccgtgccc
     4321 accaccagcg accgcaccac cgatcgggcc gcatcgtgag cgcccccctg cccgccggcg
     4381 tcaccgagcg caccatccgc gtcggcgaca tcgacatgca cctgctcgag gccggcaccg
     4441 agggacccct cgtcgtcctg gcccacggct tccccgagct ggcctggtcg tggcgccacc
     4501 agctgcccgc cctggccgac gccggctacc gggtcgtcgc ccccgaccag cggggctacg
     4561 gcggcaccag ccggccggag gcgatcgagg actacgacat ccaccacctc accggcgacc
     4621 tcgtcgggct cctcgacgag ctgggcgagg agcgggccat cttcatcggc cacgactggg
     4681 gctccatggt cgtctggcag ctgtcgctcc tgcacccgga ccgcgtcatg ggggtctgcg
     4741 gcatgagcgt gccgttcgtg ccccgggcct cggcgccgcc catccagatc ttccgggcga
     4801 tcttcgccga ccagttcttc tacatcgtgt acttccagga gccgggcgtg gccgatgccg
     4861 agctgggcgg cgacccggcc cgcaccatgc gccgcctgct cgccgggctg tcgaccccgg
     4921 gcgacctgac caccgacgag gccgcctcca tgtcggcgga cgacgggcgg ggcttcgtcg
     4981 accgcatgcc cgagcccgac ggcctgcccg actggctcac ccaggaggag ctcgaccact
     5041 acgtcgaggt cttctccgag accggcttca ccggcggcat caactggtac cggaacttcg
     5101 accgcaactg ggagctcacc ccccagctgg ccggggccaa ggtggaggtg ccctcgttct
     5161 tcgtcggcgg cgccgacgat cccgtcctca ccatgtcccc gcccgccggc caggaggcct
     5221 ggctgaccga ccaccggggc agcgtgatca tcgacggtgc tggccactgg gtccagcagg
     5281 aagcacccga gcaggtgaac gcagcgctgc tcgacttctg tgaccagctg cgtggaggaa
     5341 ccaacctgtg agcaccgccc cgtcccgact gaagttcggc gccttctacg ccccgttcca
     5401 cccgccgggc cagaacccga cgctcgccct cgagtacgac ctgcagcggg ccgaggccct
     5461 cgaccgcttc ggctacgacg aggtgtggtt cggcgagcac cactcgggtg gctacgagat
     5521 catcccgtcg cccgaggtgt tcatcgcggc cgccgcgcag cgcacgaagc acatcaagct
     5581 cggcacgggc gtggtgtcgc tcccctacca ccacccgctg atggtggccg accgaatggt
     5641 cctgctcgac cacctcaccc ggggtcgggt catcttcggc accggtcccg gtgcgctgcc
     5701 caccgacgcc tacatgatgg gcatcgaccc ggtggagcag cgccggatga tggaggagtc
     5761 cctcgaggcc atcctcgccc tgctgcgcac cgacgagccg gtcacccggg agaccgactg
     5821 gttcacgctg cgcgacgcga agctgcagta ccggccctac agcgacccgc acttcgaggt
     5881 ctccgtcgcc gccatggtgt cgccgtccgg gccccgcctc gccggcaccc acgacgtgtc
     5941 gctgctgtcg ctgtccatgt ccatcgccga cgggttcgct gccatcggcc aggcctggaa
     6001 cgtggtcgag gacatggccg ccaaggccgg ccacaaggag cccgaccggg ccaactggcg
     6061 ggtgctcggc accatgcaca tcgccgagac ccgcgaccag gccatcgagg actgcaccta
     6121 cggcctccgg gacttcgcca actacttcgg cggcggcgcc ggcttcgtgc cgctgtccaa
     6181 cgaggtcacc gagggcacca cgtccgagcg cgacttcgtg gaggcctacg tcgagggggg
     6241 caagggcaac gtggtcatcg gcacgcccga cgacgccatc gagtacatcg agggcctcct
     6301 cgagcagtcc ggcgggttcg gcacgttcct catgctcggc cacgactggg cccgacccga
     6361 ccgcaccatc gagtcgtacc gcctcttcgc cgagcgggtc atgccccact tccagggcca
     6421 gctccaggcc cagcgcgact cccacgactg ggccaccggc aagcgcaacg agctgttcgg
     6481 ccgggccggc caggcgatca tgaacgccgt gcagcagcac gtccaggagc aggacgcgga
     6541 gcaggcctga tgcgcgcggc cgtcctgaac aagggcgaga tcctcgtccg cgacgacgtc
     6601 gaggagccga ctcccgggtt cggccaggtg ctggtcgagg tgaaggcctg cggcatctgc
     6661 gggtccgacc tgcacttcgc gaagcacggc ggcgacatgc tcgccctcgg caagcagatc
     6721 gaggggatgc cgagcatggg cggtgagggc gacggggtcg atctcgaccg cgacgtctac
     6781 atgggccacg agttcgcggc cgaggtgctc gacgtcggcc ccgacaccga ggccgcggcc
     6841 ccggtgggct cgctcgtcac gtcgatgccg gtgctcctcg ggatggacgg catccacccc
     6901 atcgtctaca gcaacaccgt gaagggcggg tacggcgagc ggatgctgct gtcggccccg
     6961 atgctgctcg aggtgcccaa cgggctctcg gccgagcacg cggccctcac cgagcccatg
     7021 gccgtcgggc tgcacgccgt gaacaaggcg cgcctcgagc agggcgaggg cgccatcgtc
     7081 ctcggctgcg gcccggtcgg cctggcggtc atcgccgccc tggcggtgcg gggggtcggc
     7141 cccatcgtgg cagccgactt ctcccccgcc cgccggcggc tggccgagca gatgggggcc
     7201 accgacatcg tcgacccccg cgaggagacc ggctggcagg cctggacccg ggccggcaac
     7261 ggccgggcgc cggtggtgtt cgaggccatc ggcgtgccgg ggatcctcga cgacatcctg
     7321 cggtgcgccc cggcccagtc gcgggtggtg gtcgtcggcg tgtgcatggg gcacgacacg
     7381 atcaacccgt tcttcggcat cagcaaggag ctgaacatcc agttctgcct gggctacgac
     7441 ccgatggagt tcgccggcag cctgcgcagc atcgccgagg gcgagatcga cgtggccccg
     7501 atgatcaccg cccaggtcga cctcgacggc gtgccccagg ccttcgccga cctcggggac
     7561 cccgacgagc actgcaagat cctggtggtc ccatgaccaa gccgatgcac gagacgcacc
     7621 ggatcgcgta cgacggcgcg gtcgacgccg acggccacat cctcgagccg ccggacctgt
     7681 gggagaccta catcgacccg cagttccgcg accgggcgct gcgcatccgc accgacgagc
     7741 agggcctgga ggagctcgag atcggcggcg agcggtcgca gatggctcgg cggggcatgc
     7801 catcgacgct cggggccatg ggcgcgcccg acctggccgc catgcagaag gaccccgagc
     7861 gcacctacct gcgcgaggcg ccgtacggga gcatggaccc ggcccagcgg ctcgaggtgc
     7921 tcgacgccga gaacctcgat gccgccatcc tctacaccac cgtcgggctc ctctgggagg
     7981 ccgagctcac ggacccggag ctgtcgcagg cctacaccaa ggcctacaac cgctggatct
     8041 gcgagttctg cgccgactcg ccccgcctgg tccccacggc ccacctgtcg ctgtccgatc
     8101 ccgtcgccgc ggcggccgag ctggagcggg ccatcggcga gggcgccaag ggctgctacg
     8161 tcgctccgtt cacccacgac gccaagcccc tcggccaccc ggacaacgac ccggtcttcg
     8221 ccgtggccca ggaggccggc atcccctttg ccatccaccc gaccttcgag ccgcagtgga
     8281 ccaagggcca gcgcatgggc acgtgggaga acgtcaagca gctgcgcctg ctcgcctcgg
     8341 tcaccgcgtc cgacggcgtg cgacagcagt tcaccacgct gttcgactac ggcgtgttcg
     8401 accgcttccc cgagctgaag gtgctggtgc tcgagtccgg cggcgggtgg atcggctact
     8461 ggctcgatcg catcgacgcc gtctacggcc acacgttcat cggcgagcgg gtgccgctgc
     8521 aggagaagcc gagcgactac ttccggcgcc agtgctggat cagctgcgac cccgacgagc
     8581 gcaccatccc cgccctcgcc gaccgcttcg gcgtcgaccg gttcctgtgg gcgagcgact
     8641 tcccccacgc cgaccacacg cccgagtacg tcgaggacct caacgagctg gccggcatgt
     8701 tctccgacgc cgatcgccgc aagttcctgg gcgacaacgc ccgggcgctg ttcgacatcg
     8761 acgcctgagg attctggcag cggccacgga acgggcgttc cgcagtcgct gccgggagcg
     8821 atcggtacgg tgcccggcca tggctggagc agcgggcgcc cgcaagcggg tgcgcatgga
     8881 ccacgaggag cggcgcaagc agatcctcga cgcggccacc gcgctgttcc tgcgccggcc
     8941 ctacagccag gtctcggtgt ccgacatcgc cgaggccgcc ggcgtggcca agggcctgct
     9001 gcaccactac ttcgactcca agcgcgacct ctacctcgag gtcgtccggg acgtgtccag
     9061 cgtccccctg ccggtcggcg cgcccgccgg cccgggtgag gacccggcgt gggcggctgc
     9121 catcgacgcg ctgctcgcct tcgtctcgga gacccgggag ctgtggctgc gcgccaccac
     9181 cgtcggcgac gacgaggtcg cctcgatcgt cgacgacagc cgcgaggtgc tggccgagca
     9241 gacgatcgag gcgctcggac tcgacgacac gcccgtcgtg cgggccctgg tgcggggcta
     9301 cggcgggctg gtgcaggagg tgacgcgcga gtggctggac cgcggtcggc tgaccgaggc
     9361 ccaggctcgc gaggtgctgg tgcggacgct cccgctcctg ctggagcggg tgctgcccga
     9421 cctcaggccg tgagggcctt gatcttctcc tggaaggcga cctgctcggg cgacggtgcg
     9481 ccggccgact gcatggccat gagcttggtc atgtcgccgt cgatcttgat ctgaccggac
     9541 atgaaggcct gcatggcggc ctgctggtcg ccggcgatga agacgctctt ggccacctcg
     9601 tagggcacgg tcagcttcgt cggggccgag tcgtgcaggc cccgctcgaa ctggccgccg
     9661 gccatgtggg cctcccgctc gccgaagggc gtgtcgggca cgacgaggtt gatcacgagg
     9721 tccttggtgg ccgcgggcgg ctcgggggcc tccgggcgca gcgcctcgat cgcgtccatc
     9781 cactcatccg acaagaagtc gtgtgccatc gctgttgctc ctgttgctcg ctggggtgcg
     9841 ccgacgcggg cggcgccacg accttgttgg ccgctggcca acaacttcgg cacgccgggg
     9901 aggaagtcct acgcccggcc ccgaaaagaa cctcgtcagg gggcaggaaa caaccgttgc
     9961 cagatcgaag tgtggcatcg ggcacatgtt cgagccacag ggggatctgg ggatgggcga
    10021 gcgacgacga gcgatcaggc ggggtgcgcg cccccgggcg cggtaccggc gcacgcacgt
    10081 gaccgcggcc gcatcggtca cgatgctcgg cctcagcgtg ctgctggccc cgggcggggt
    10141 ggcccaggag gtcccgcagt ccgcgccgga gtcgggcgag atccgcaacg gcacggccaa
    10201 ggcggtcgcc accgtcgcca agatcgcccc cggtgtgggc cagctcgagc tcggcatcac
    10261 catgggcacc gccgtcaccc agctcacgaa cgacctggcc caggccaccg cccagaccgc
    10321 cgacctcggc ctcatcggct cctcgctcac gtccgagggg tgcgacggcg acgccagctt
    10381 cacccccgac cagctgcccc agcccacgtc ggtggacaac cgcaaggggg acgcctcgca
    10441 gagccgggac gagggcggca ccgacggcgc gccggtcgcc ctcggccgca tgcaggtgga
    10501 ggcgaccgag gacgcgcccg catcccgggc cgtggccagc tccaccatcc tcggctcccc
    10561 cgacctgctg cgcatcggcg gcggcgacgc ggagtccagc acccgggtgc tgccgggcga
    10621 agggcgggag gccaccgcga cggtcacctc ctcgctctcc atcaccgacg ccgtgcgcct
    10681 ggacggcatg cgctggcacg ccacccaccg caccggtgtc gacccgctgg ccgagggcgc
    10741 cttcgagctg ggtcgggccg aggtcggcgg cgttcccctg cccaccgagg acctggcgtc
    10801 ggccgaggcc gcgatcaacg aggcgcttgc gcccaccggc atcagcatct cgctgccccg
    10861 cgtgcagcgc atgctcgagc cgaccgacct catccgcgtc acgccgatgc gcatcgagat
    10921 ccgcgactcc ccgctcggca agaccgccct cggcccggcg ctggacgcca cccgggatca
    10981 gcgggtgcag ctgttcgacg acctggttga gctgtactgc gacagcgcca gcgtcctgct
    11041 cgtcggcgac atcggcatct cggtcgtggc cggcaccggc ttcctgaccg tggacctggg
    11101 cggcgtgcag gcatccagct ccgacctgga ggtcggcaac ccgttcggca cgatcgagcc
    11161 cttcgagctc ggcggcgtct cgatccccgg caccggcgtc gacacctcga ccgccgcacc
    11221 gcccgccggg tctcccccgg tcgccggcgt ctcgccaccg agcaccgtgg ctgcggcacc
    11281 ggccgtcggc gcggtgccgg cccgcgccac cggtccggtc gaggagctgt gcgagtcgct
    11341 gcaccccaac cggggcacct gctccgaggg cgcagccgcc gccgtcggcg tggtcggcct
    11401 gctcgccacc gtgggcatgg ccggcgccga cgtcctccgc cagcgacgcc aggccgccct
    11461 cgccgaggac ctcgcatgat cggctccctg ctcggccacc gcggcctgcg cccgtacctg
    11521 ccggtgggcg ccgccgccgt cgccttcctg gtcatggcct ccgtcgtcgg tccggcgccg
    11581 cgcagccacc aggcgctcgc cggctccgtg gaggtcccgc tcgccgccgg accccagcag
    11641 tccggccccg gagccggcga cgccggtgcg cccggttcga ccgcgccggg cgccgacggc
    11701 accgaccccg gcggtccggg cggtgccacc cccgggacgg ccaccgaccc gcagagcgac
    11761 ggaggtggcg gcgtcacacc gtgccccgac cgcgccgcac aggtccccgg cgacccgtac
    11821 tccccaccgt gcctcgtctt ctccggcgac aacggcgggg ccaccaccac cggcgtcacg
    11881 gcggacgaga tcgtcgtcag cgtgcgccgc ctcgagggcc cgacggcggg ggagatcttc
    11941 gccgacatct ccggcgagtc cgtggaggac agccccgagt cctacgccaa caccctcgac
    12001 gccctggcgc agtacttctc cgagcgcttc cagttctacg gccgcagcct gcgcatcgag
    12061 atctacgacg gcgagggcgt cggctcctcc gagctgctcg gcggtggccg cgaggccgcc
    12121 ctggccgact cggtccgggc gcgggagatc ggtgcgttcg ccgacctgag cgccatcacc
    12181 ctcccctacg cggacgccct cacccgccag ggcatcatcg gcttcggcgc cccctacccg
    12241 tcccgcagct ggttcgagga gcgccgcccc tacgcctgga gcctgttccc cgacggcacg
    12301 ctcgtcagcg aggccggcgc cgcagccacg gcggcccgcc tcgccggcca gcagaccgcg
    12361 gagtacgccg gcgaggacat caagggccgc cagcgggtgt acggcctggt cgcccccgag
    12421 aacgccgagt accaggagtc ggtcgagcgc tacatctcgg ccatcggcga gcacggcatc
    12481 accttcgcca agagcctccg gtacaagctc gacatctcct cgatgccgaa ccaggcctcg
    12541 aacatcatcg cccagctcaa ggacgccggc gtgaccacgc tggtgtgcgc gtgcgacccg
    12601 gtcatgctcg ccctcggcat ggcccccaag gccaacgagc agtcgtggga gcccgagtgg
    12661 ctcaccggcg gcctcgcctt cgtcgagcag gacatcgtcg cccagctcat cgacagccgg
    12721 caatggcggc acgccttcgg caccgccttc aacgccgaga gcgaaccgat cggggcgtcc
    12781 tacccctacg ccgccttcaa gcagatccgg cccggcgacg agcccgcctt cggcgtcgag
    12841 gagatctact actcgatgta cctgctggcg ctcggcatcc acctggccgg gccgaacctc
    12901 acgcccgaga gcttcgaggc cggcatgttc tcctaccagg gagccagcgg cccgaggggc
    12961 tactggagct tcggcccggg tgactacacg cccaccgacg acttccgcga gatctggtgg
    13021 gacccggacc gcatctcggc ccagaacaac aagcccggcg cctgggtgca ggtcaacggc
    13081 ggccagcgct ggttcgccga cacgatcccg acgggccccg ccccgttctt ccaggagggc
    13141 tgagatgccg accaccaccg aggccctcga ggtcctgcgc cggcgccccg ccgccaccga
    13201 gctgttcggc ggcttcggcc cgctcgtcct ggccgccgcc ctgctcctcg ccatggtgct
    13261 gctcgccccg tcggtggcgc cggagcgcac cgtcgagcgg ccggtcgagc ccggcaccag
    13321 cacggaggcc ggacggtgat cgcccgcacc gccgagcggc tgcgcaccga ccgggccagc
    13381 caggtggtgc tcgccaccgc cgtcgcctac accctcctct actgggtgcc cctcagtccc
    13441 ctgccccgac tggtcccgtg gggcgtgatc gcccagggcg tcatcttcgg cacgtcgtac
    13501 gccctgctgg ccatgggcct catcctcatc taccgcacga cccgcatcgt gaacttcgcc
    13561 tacggggcca tgggcgcgat gccgggttcg ctcaccgtgg ggctcttcgt ggccaagggc
    13621 tggagctact gggtggcgat cctcgtcggc gtggcggtcg gcgtggccac cggtgccgcc
    13681 gtcgacgtct tcgtcatccg ccgcttcgcc cggtcctcgc gcctggtgct cacggtcgcc
    13741 tcgatcgggc tcgcccagat cctgggggcg gtcgggctca tcatcggcgt cgccctcggc
    13801 accgacgccc tcatcggcaa catcgacaca ccgctcagcg gctcctggtt cgtgcgcccc
    13861 tacccgatcc gcggcgacca cctcctcatg ctggcggtgg cccccctgac cctcgccggc
    13921 ctggggtggt tcctgctcgg caccgacacc ggccgggccg tgcgggccgc ggcggagaac
    13981 gaggaccgcg ccctcctgct cggcgtgccg gtgcggcggt tgcagacggt cgtgtgggcc
    14041 atcgccggtg gcgtgtccac catcaccttc gtcaccaagg cgcccttcac cggcgtcgtg
    14101 cccgacgcgc tggtgggtgc cacgacgatc ctgcccggcc tggccgtggc ggtcatcgcc
    14161 cgcttcaagt cgctgccgct cgccctggtc ggcggcatcg ggctcggcat cgccgagtgg
    14221 accatccgct ggaacgtccg ggccgagtcg gtgttcgacg tggccttcct cgtcgccatc
    14281 ctcgtcgccc tgctcctcaa ccgggagcgc accacccggg ccgagagcgg agagagcagc
    14341 tgggacgccg ccggcgtgct caagggcatc ccccgcgagc tgcgcgacgt gcccgaggtc
    14401 cgggtgccga tgcgggtgct cgccgtcgtc ggactcgctg ccgtcgtgct gtacccgctc
    14461 tccttcgccc cctccacgat ggtcacgctc accttcggcg tgctgtggtg catggtcggc
    14521 gtgtccctcg ccgtgctcac cggctggggc ggcagcatct cgctcggcca gttcgccatc
    14581 gtcggcgtgg gcgccatggc caccggcaac gccctgatcc ggtggaacct cgacgtgttc
    14641 gcgtcgctcg ccctcgccgt ggccgccggc gccatcgtgt cggtgctcgt cggggtgccg
    14701 gcgctgcgca tcaaggggct ctacctggcc gtgacgacgc tcgccttcgc cgtcgccctc
    14761 gactcgtact tcctgaaccc ggtgaacttc cccgagctcg tgcccgaccg catcatccgc
    14821 ccggtgctgt ggaagcgctt cgacctcggc agcgagtgga ccacgtacta cctgtgcctg
    14881 gccacgctcg gcgccaccgt cctggtcgtg cgggccatgc gccgccgccg ggccggtcgg
    14941 gtcatggtcg gcgtgcgcga caacgaacgt ggtgccgccg ccctcgccgt gcccaccacc
    15001 cgggtgaagc tgcagacctt cctggtcgcc ggctgcatcg ccggcctggc cggcggcctc
    15061 tacgtggtgg tcgccggcgg caccggccag ggcacgttcc ggcccggcat gtccatcgag
    15121 gtgttctcct tcgccgtcat cggcgggctc ggctcggtgg ccggcgtcat ctcgggcgtc
    15181 gtgatgttcc gcctgctcga cttcgtgctg gcccggggcg tcgacggcgc cgccgccgac
    15241 atcctgcgcc tcagcctcac cggcggtggc ctgctgctga tcctgtactt cctgcccggc
    15301 ggggtgtggc agtacgtgca gcgagcccgc gaccgctacc tgcgggtggt cgccgaccgg
    15361 cgcgggatcc tcgtgcccag cctcgtcgcc gaccggcgcg tcgaggacgg cgcgaaggac
    15421 gaaccggagg acgagacgtc cgtgatctcg ggagccctga cgtgagcgaa cccctgctgc
    15481 gggtcgaggg cgtcgacgcc gcctacggcc ccgtccagat cctcttcggc ctcgacgtcg
    15541 acgtgcacga gggcgagatc gtcgccctcc tcggcaccaa cggcgccggc aagtcgaccc
    15601 tgttcaagtg catcaccggc ctgctcccgc ccaccgacgg caagatcctg ttcaagggcg
    15661 aggacctggt cgggcgcacc accgacgaga tcgccgagct gggcgtggtg atgatgccgg
    15721 gcggcaagag cgtgttcccc acgctcacgg tccgggagaa cctgcgcctc gcgtcgtggc
    15781 tgaagcgcaa ggacaagcag gccgtgctcg acggcgaggc ccgggtgctc gagctgttcc
    15841 cccgcctcgc cgagcgcatc gaccagctgg ccggcaacct ctccggcggc gagcagcaga
    15901 tgctcgccgt cagccaggcg ctgatccccg accccgagct cctcctcatc gacgagctct
    15961 ccctcggcct cgccccgacc atcgtcgggc agctcatcga cgtcgtgcgc caggtccacg
    16021 ccagcggact caccgtcgtg gtcgtcgagc agtccatcaa cgtggcgctc acgctcgccg
    16081 agcgggccgt gttcatggag aagggcgagt tccgcttcac cggctccacc cgggagctgc
    16141 tcgagcgacc cgacatcctc cgctcggtct tcatcagcgg cggcaccgac agcagcgggc
    16201 ccgattccga gaccgccgcc ggaacgcccg gcaccgtccc ggcgacgccg gtgcgcaccg
    16261 cgcccgagcc cgacgccccc gccatcctcg agtgccacgg cgtcaccaag cgcttcggcg
    16321 gcatcaccgc cgtcgaccac gtcgacctcg agctgcgcca gggcgagatc ctcggcctca
    16381 tcggccagaa cggcgctggc aagaccacac tcttcgactg cgtgtccggg ttcctgccca
    16441 tcgacgaggg gcgcatcctg ctgcggggcc acgacatcac ctcgctggcg ccccacgagc
    16501 gggcccggcg gggcctgggg cggtcgttcc aggaggcccg cctgttcccc accctcacca
    16561 ccgccgagac gctggcggtc gctcgggagc gccacgtgcg ggggacgtcg atggtcgccg
    16621 acgcgttcgg ccagcccgtg gcccgggcca ccgacgacga catcgccacc gcggtcgacc
    16681 tgctcatcga gctgatgggc ctcggtgcgt tccgcgagaa gctcaccggc gagctgtcga
    16741 ccggcacccg gcgcatcgtg gagctcgcct gcctcctggc ctcggacccc aaggtcatcg
    16801 tcctcgacga gccctcgggc ggggtcgccc agcgcgagac cgaggccctc gggccgctgc
    16861 tccagcgggt gcaggccgcc accggcacct cggtcctggt catcgagcac gacatgccgc
    16921 tgctcaccag catctgcga
//
LOCUS       DATISU010000365         1897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_401760, whole genome shotgun sequence.
ACCESSION   DATISU010000365 DATISU010000000
VERSION     DATISU010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401760"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VLR27_14540"
     CDS             <1..475
                     /locus_tag="VLR27_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HSP04727.1"
                     /translation="VPHIYVAGDLSGRLPLSSVASMQGRKIAEHVMGLHTREHRHLDY
                     DKAASAIFTEPEIADVGLAEADAFAEGRKIRVTKVPFSTTAKALINNAPRGFVKIVSD
                     PATGVVLGGSIVGRHAAELISVIALAVTAGLKVEDIVDSLLVHPSLSEALAEAAD"
     gene            475..1134
                     /locus_tag="VLR27_14545"
     CDS             475..1134
                     /locus_tag="VLR27_14545"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP04728.1"
                     /translation="MPYTEITAEQFTATDGLDDWRVVLRTAEASFRAPSFPAAGRLVG
                     AIADAAEAANHHPDVSLTYPGVVRVALTTHAVDGLSTHDTDLAHHISQLAADAGATAE
                     PMAAQILEIAIDTMDADRIRPFWAAVLDYREQPDGSLSDPRGFGAPVWFQQMDEPRTE
                     RNRFHLDVSVPHDLAEARLEKALAAGGTLVTDEHARAFWVLADAEGNEVCICTWQDRS
                     G"
     gene            complement(1159..>1897)
                     /locus_tag="VLR27_14550"
     CDS             complement(1159..>1897)
                     /locus_tag="VLR27_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HSP04729.1"
                     /translation="AEGKFLPSPGPIATLVPPQGNGVRWDGGYDSGDEVSQYYDNLIG
                     KLVVWAGDRDTAIARMLRCLDEFVLDGPPTTIPAAEAILAHEDFKAGEHSTKWVEDTL
                     DLTGVRGTPAPTGDDEDDKVERNVDVEVNGKRFAVKLFVPADQVAVAAAGGGGGLPKA
                     RKRSKGTSGAAPTAGSGRIAVPMQGTVVKVNVAVGDEVTEGQVLCVLEAMKMENNIAA
                     DKAGTVSEVNVEEGASVGSGDVIIVIE"
BASE COUNT          260 a          707 c          653 g          277 t
ORIGIN      
        1 cgtgccccac atctacgtgg cgggcgacct gtccggccgg ctgccgctgt cgtcggtggc
       61 gtcgatgcag ggtcgcaaga tcgccgagca cgtgatgggc ctgcacaccc gcgagcaccg
      121 ccacctcgac tacgacaagg ccgcctcggc catcttcacc gagcccgaga tcgccgacgt
      181 cggcttggcc gaggccgacg ccttcgccga gggccgcaag atccgggtga cgaaggtgcc
      241 gttctccacc acggccaagg ccctcatcaa caacgcgccg cgcggcttcg tgaagatcgt
      301 ctccgacccg gccaccggcg tcgtgctcgg cggctccatc gtcggacgcc acgccgccga
      361 gctgatctcg gtcatcgccc tcgccgtcac cgccgggctg aaggtcgagg acatcgtcga
      421 cagcctcctg gtccacccca gcctctccga ggcgctcgcg gaagcagccg actgatgccc
      481 tacaccgaga tcaccgccga gcagttcacc gccactgacg gactggacga ctggcgggtc
      541 gtgctgcgca ccgccgaggc cagcttccgg gcgccgtcgt tcccggctgc cggccgcctg
      601 gtcggtgcca tcgccgatgc tgccgaggct gcgaaccacc acccggacgt gtcgctgacc
      661 tatcccggcg tcgtccgggt cgcgctcacc acccacgcgg tcgacgggct ctccacccac
      721 gacaccgacc tggcccacca catctcccag ctcgctgccg atgccggcgc caccgccgag
      781 ccgatggcgg cccagatcct ggagatcgcc atcgacacga tggacgccga ccgcatccgg
      841 ccgttctggg cggcggtgct cgactaccgg gagcagcccg acggcagcct gtccgacccc
      901 cgggggttcg gcgcgccggt ctggttccag cagatggacg agccccgcac ggagcggaac
      961 cggttccacc tcgacgtctc ggtgccccac gacctcgccg aggcgcgcct cgagaaggcg
     1021 ctcgccgccg gcggcacgct cgtcaccgac gagcacgccc gggcgttctg ggtcctcgcc
     1081 gacgccgagg gcaacgaggt ctgcatctgc acctggcagg accgctccgg ctgagcaacc
     1141 gggcggcgcc cgccggcgct actcgatgac gatgatcacg tcgccggagc cgacggacgc
     1201 gccttcctcg acgttgacct cggagaccgt gccggccttg tcggcggcga tgttgttctc
     1261 catcttcatg gcctcgagca cgcagaggac ctggccctcg gtgacctcgt cgccgacggc
     1321 gacgttgacc ttgacgacgg tgccctgcat gggaacggcg atgcggccgg aaccggcggt
     1381 gggcgccgcg ccgctggtgc ccttggagcg cttgcgggcc ttgggcagac cgccaccgcc
     1441 gccggcggcc gccacggcga cctggtcggc gggcacgaac agcttcacgg cgaagcgctt
     1501 gccgttgacc tcgacgtcga cgttgcgctc gaccttgtcg tcctcgtcgt cgccggtcgg
     1561 ggccggggtg ccccggacgc cggtgaggtc gagggtgtcc tcgacccact tggtggagtg
     1621 ctcgccggcc ttgaagtcct cgtgggcgag gatcgcctcg gccgcgggga tggtggtggg
     1681 cggaccgtcg aggacgaact cgtcgaggca gcgcagcatg cgggcgatcg cggtgtcgcg
     1741 gtcgccggcc cacacgacga gcttgccgat gaggttgtcg tagtactggc tgacctcgtc
     1801 accggagtcg tagccgccgt cccagcgcac gccgttgccc tgcggcggga ccagcgtggc
     1861 gatggggccg ggggagggga ggaacttgcc ctcggcc
//
LOCUS       DATISU010000366        11130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_494800, whole genome shotgun sequence.
ACCESSION   DATISU010000366 DATISU010000000
VERSION     DATISU010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11130
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494800"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1687
                     /locus_tag="VLR27_14555"
     CDS             56..1687
                     /locus_tag="VLR27_14555"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HSP04730.1"
                     /translation="MTAPVDERRSLARSTATQTVLTAVSRITGFVRVVVVAAVLGTTF
                     LGNTYQSANTIPNIVFELFAAGALQAVLVPALVARFDRGDDEEAERLAGSVLGLTLAV
                     LAGLAVLGALASPLLMRALVSGVDDPAIRADQVALGTIFLLVFLPQILVYDVGLVATA
                     VLHARGRFALPAIAPAVNNLVVCGAYGLFWWMRRGEEPSLDLSAAEIAVLAGGTTFGV
                     VAFCALPIVGARRAGFRFRPNLDRSDPALRFLARQGGWAAIYLAMTQVFLGAVLLFAN
                     RTEGGVIAYQVGFTFFLLPHSLFALPVLTTLFPAAARHVHAQRWAEVRATVRRGTTAI
                     ALFVLPSAVALGVLAEPLARVASFGNVGQGGAELIADVVRGFAPGLVGYALFYFLTRM
                     LYAMEDTRTPATAHAVVVVLGVLAMAVSAATLDGDQLVAALAWAHSGTYLLGALLLGA
                     VVTRRLLRRDEPIHLLAALGPQLAGAAVASAAVLGVVASLSWDSRLGSLGIVVLGAAA
                     VAVVHITVQSLLGGPRPMAIVGLLRGAPAAGEGST"
     gene            1684..2754
                     /locus_tag="VLR27_14560"
     CDS             1684..2754
                     /locus_tag="VLR27_14560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSP04731.1"
                     /translation="MSRPVALELLGPSIGGIRRHVSYLSEQLRAAGWEVRTAGPAGVL
                     DDLDHVVPIPGGLRIDQVLAARRALRPLLEEVDVVHAHGLKAGWVAATVRPRPPLVAS
                     IHNLVLDDVAGRSAPLLRLLEERLPGRVDATIAVSREVARRFAGRPGAERIHVIPPAG
                     PPPVPARSAEQVRAELGVGEDEDLVVTAARLHAQKGLHVLLDAAAAIRTQRPGLRWFV
                     FGDGPLRGELEEAISARGLERVVHLAGSRPDVDSELAAADLVVVTSQWESGPLVVLEA
                     LALGRPVVSTDVGLVPEVIDPGSGRVVPVGDAVRLAEAVVAVLAGSSAPEGEVPGSSA
                     RFRPEALAAAVRDVYEEVMSPR"
     gene            2751..5063
                     /locus_tag="VLR27_14565"
     CDS             2751..5063
                     /locus_tag="VLR27_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04732.1"
                     /translation="MKPLRIAAALAVVAVAAVLLVVPGRGSTSRADADRVLIVSIPTL
                     TWDLVADEQPPNLVSLLARSAVASASVRTVGIDTDLGEGYATMGSGNRTAVVTEYASL
                     AFPPDATFEGEPVADVYERRFGTAPGDAEVVQLGAAAIQRRNDDLLYGAEVGSLGAAL
                     RDAGRSAAVVANAELVVDPPVSELSRQAALAVMDDLGRVARGRVDGGLLEAGPDLPGG
                     VALDADQVLAEVESAWDDSDVLMVELSDLLRAEATEPVVTPEVADAVAADALARTDDL
                     LGALLEGVDPERDLVLVVSPVSPVGPDNLVVAAAAGAGLEPGLARSGTSRRDGYVTLP
                     DIGPTVLDALGIDKPGAMSAAPIASSGGGAPDAGTFRGLADDNVRALFRNSLSGPLTV
                     LFIISQILTYAVAAYALSSHRRQMAGTVAFLLLAGMALPFVSFLAGVFPYWRLSVGGA
                     IAAIFAVSGIVAAVAHLVSARAVDSGRVHSRALVPSVLIMSATVVLLVGDIVLGGPLQ
                     IDTAFGYGGGAIVAGRLAGYGNLAWALMVAALIIAATGIWGRRELSGGVGRDRGDRRF
                     DLAVVGGVFVLAVLAVGLPALGANVGGTISVVSGLAITLLALTGARIDLRRLVGVGVL
                     TALVLAAFGALDLRRDPQDQTHLGRLIDQTLGDEGIAGLSTVIERKINANLNILLSSV
                     WSLVIPAALAFLAFLIWRPPRFLRTVFQRIPGVRACVIGVLATGVIGGIVNDSGIAIP
                     AVMLTLLLPHVAYLVVRTHDAAVVDAEPET"
     gene            5060..5914
                     /locus_tag="VLR27_14570"
     CDS             5060..5914
                     /locus_tag="VLR27_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04733.1"
                     /translation="MNDAVVLAVALGLGVAGGALAWPMLAQVFAAPLFLRTNVRGAEV
                     PVAVGIVLPVVLLAAGAALVVLDAGDLVQVPQGALAVSVLAAAGFGMLGLFDDLAGDR
                     AAQGFRGHLSALRSGQLTTGAVKLFGGGALAVLVAAPTARERFWVLLADAALIALSAN
                     LANLFDRAPGRLAKVSLLATVPIAVVAGIDPDLAGPAVVLGALLALLAPDLRERLMLG
                     DTGANVLGATLALAVVLTTAPSTRVVVLVVVAALNLLSERVSFSRVIQQVRPLRWFDG
                     IGRLPPTT"
     gene            5935..6852
                     /locus_tag="VLR27_14575"
     CDS             5935..6852
                     /locus_tag="VLR27_14575"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HSP04734.1"
                     /translation="MPDFLVIGAARSATTSLHYYLDQHPGITMSRIKEPNHFAFDHSV
                     DPPSPLIDPASPIVTKSVPDRHAYEQLFAHAGPGDVLGEASPLYLYVREAPEQIRRAL
                     TDPKLVAVLRNPVDRAYSHWLHIHRDRPEDAVAAFARGAEREIAEPPGYSPYAGASHV
                     LRMGLYDEQLQRHLDVFGPDALLVLSYDEVTVRPGESLERICDHLGVARHDFDTGVQY
                     NRSGVAGGALRARVGRVVRAVQPTVKRVLPDTVAARLGRLRAERDRPDAAPPIPADLR
                     ARLVDWYYPSVERLRAMDVVDISGWDDFT"
     gene            6899..8566
                     /locus_tag="VLR27_14580"
     CDS             6899..8566
                     /locus_tag="VLR27_14580"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HSP04735.1"
                     /translation="MTKHIFVTGGVTSSLGKGLTASSLGRLLKARGLRVTLQKLDPYI
                     NVDPGTMNPFEHGEVFVTDDGGETDLDLGHYERFIDESLTKDSNATTGSIYSAVLAAE
                     RRGDYLGKTVQVIPHITDEIKRRISRLANDDVDVVITEVGGTVGDIEILPFLEAIRQF
                     RLDVGRGNVCYVHVTLVPYIGPSGEQKTKPTQHSVTELRSRGIQPDAIVVRSADPIPD
                     DLKHKISRLCDVHADGVVNAADAGNIYEIPLVLHEEGLDSVVCDVLQMTDLPDPDLRE
                     WEALVAKVEAATRPVRIGIIGKYVALQDAYLSVVEATKHGGFDIGVDVEFEWIQAEEV
                     EGLMADGRLRDLDGVVIPGGFGERGTEGKIEAARYTRENEIPCLGLCLGLQMMVVDYA
                     RNVLDMPGANSTEMEPAAVDPVITIMPSQHDVTDMGGTMRLGAYVAQLTPGSKVAEAY
                     GTTVVSERHRHRYEFNPRYRSRFEASDLLLCGESPDGRLVEFIELQGHPFWVGTQAHP
                     ELKSRPTRPAPLFRAFAAAALARAEGRKPHLFDLDAPVEQPSSVSAD"
     gene            8567..9100
                     /locus_tag="VLR27_14585"
     CDS             8567..9100
                     /locus_tag="VLR27_14585"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSP04736.1"
                     /translation="MTSFRLQGEEAVYDGHVMRVVVGTFEGPDGDTFTRDIIRHPGAV
                     AVLPLHEDGTVTLVRQYRAPLDEHILEIPAGIRDVEDEPTEQTAVRELAEEVGLEAEH
                     LEHLVSFHNAPGMSDEVVEVYLATGLRSVDADAQGPEEEAMTIERHHLDDLATMIGDG
                     QLTDAKTVIAVALVRAR"
     gene            9097..10032
                     /locus_tag="VLR27_14590"
     CDS             9097..10032
                     /locus_tag="VLR27_14590"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HSP04737.1"
                     /translation="MTASDADEQLPLEIEEYLTWLRLERGRARSTVEAYRRDLRAWAE
                     HLHHLGRAVTEVTEDDLVAWVGDLRERGLAPSTRKRRVVAARNLHRYLAEEGHVDDDV
                     GADVAPPPVARGLPKALTEAEVDALLSRVVGDDPVARRDRAILETLYGTGVRISELVS
                     MSLRDLDLHDGFVRVVGKGDKERVVPVLGAAADALGAWLTVDGRGAMIPARWRRRDDE
                     EAVFLNQRGGRLGRQGTWLVVKAHAAEAGLGDRLTPHVLRHSCATHLLDHGADIRAVQ
                     ELLGHASIATTQVYTKVSTERLVAAWRSAHPRAGA"
     gene            complement(10034..10681)
                     /locus_tag="VLR27_14595"
     CDS             complement(10034..10681)
                     /locus_tag="VLR27_14595"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HSP04738.1"
                     /translation="MAAGWETGEVIVRREVLGLAPGFHPDPPPPWYGRPWEALPVYVV
                     RDDAEHLVTYIAEGAEMGFVDGPWPTPDGLHPWSRKQAWGGHGCLMVQRPGDPWAVWH
                     FWKGPDREFACWYVNLQADFVRTSIGYDTQDFELDLIVYPDGSLVVKDLEVLDDRVAE
                     GRYTAGLVDWIRSLGDDLVGRLEVGERLWDDRWSRWEPPSSWRGPRLPEGWATVD"
     gene            complement(10737..>11130)
                     /locus_tag="VLR27_14600"
     CDS             complement(10737..>11130)
                     /locus_tag="VLR27_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HSP04739.1"
                     /translation="QVRPAEAVAAALVGHVRRVVVDSAGTVIDLGRRQRLFTGSARDA
                     AFLQALLRGPGGLGCLWPGCDGRGKNLQVDHRDPACRGGPTNTDNSDAYCGFHNRTKE
                     HGFRPVRDPDGTWTIHRPDDDGPITPPV"
BASE COUNT         1360 a         4240 c         3888 g         1642 t
ORIGIN      
        1 gccactacgg cttgggcgag ggcgctgccg cgctcctgcc gccgctccag gacccgtgac
       61 cgcccccgtc gacgagcggc gcagcctcgc ccgctcgacg gcgacgcaga ccgtgctgac
      121 ggcggtgagc cgcatcaccg gcttcgtccg cgtcgtggtc gtggctgccg tgctcggcac
      181 cacgttcctc ggcaacacgt accagtcggc caacacgatc ccgaacatcg tcttcgagct
      241 gttcgccgcc ggcgcgctgc aggcggtgct ggtcccggcg ctcgtcgccc gcttcgaccg
      301 gggtgacgac gaggaggccg agcgcctcgc cgggtcggtg ctcgggttga ctctggccgt
      361 gctggcgggc ctggccgtcc tcggtgccct ggcctcgccg ctgctcatgc gggcattggt
      421 gtcgggggtg gacgatccgg cgatccgggc cgaccaggtc gcgctcggca ccatcttcct
      481 gctcgtgttc ctgccccaga tcctggtgta cgacgtcggc ctcgtggcca ctgccgtgct
      541 ccacgcccgg ggccgcttcg ccctgcccgc catcgccccc gcggtgaaca acctggtggt
      601 gtgcggcgcc tacgggctgt tctggtggat gcggcgaggt gaggagccga gcctggacct
      661 cagcgccgcc gagatcgccg tcctcgcggg tggcaccacc ttcggggtgg tggcgttctg
      721 cgcgctgccc atcgtcggcg cccgccgggc cggcttccgg ttccggccca acctcgatcg
      781 ctccgacccc gccctgcgct tcctcgcccg gcaggggggc tgggccgcca tctacctggc
      841 gatgacccag gtcttcctcg gcgccgtgct cctcttcgcc aaccgcaccg aaggtggcgt
      901 catcgcctac caggtcgggt tcacgttctt cctgctgccg cactcgctct tcgcgctgcc
      961 ggtcctcacg accctcttcc cggccgcagc gcggcacgtg cacgcgcagc gctgggcgga
     1021 ggtgcgggcc acggtccggc ggggcacgac cgccatcgcc ctcttcgtgc tcccgagcgc
     1081 tgtcgccctg ggagtgctgg ccgagccgct ggcgcgggtc gcatccttcg gcaacgtcgg
     1141 ccagggcggt gccgagctca tcgccgatgt ggtgcgtggc ttcgcccccg gcctggtggg
     1201 atacgcgctc ttctacttcc tgacgcgcat gctctacgcc atggaggaca ccaggacacc
     1261 ggccaccgcc cacgccgtcg tggtcgtgct cggcgtgctg gccatggccg tctccgccgc
     1321 gacgctcgac ggcgaccagc tcgtcgctgc gctggcctgg gcccactccg gcacctacct
     1381 cctcggtgcg ctcctgctcg gtgcggtggt cacccgccgg ctcctgcgcc gggacgaacc
     1441 gatccacctg ctcgccgcgc tcggccccca gctggccggt gcggcggtcg cgtctgccgc
     1501 ggtgctcggg gtggtcgcct ccctgtcctg ggactcgcgc ctcggctcgc tcggcatcgt
     1561 ggtcctcggc gccgccgccg tggccgtcgt gcacatcacg gtgcagtcgc tgctcggcgg
     1621 gcctcgaccc atggccatcg tcgggctgct gcggggcgca ccggcggcgg gggaggggtc
     1681 gacgtgagca ggccggtcgc cctcgagctg ctgggtccgt ccatcggcgg gatccgccgt
     1741 cacgtctcgt acctcagcga gcagctccga gctgccggct gggaggtccg caccgccggt
     1801 ccggccggcg tcctggacga cctcgaccac gtcgtcccca tccccggcgg cctccggatc
     1861 gaccaggtgc tcgcggcccg gcgcgccctg cgcccgctgc tcgaagaggt cgacgtggtg
     1921 cacgcccacg gcctcaaggc cggctgggtg gccgccaccg tgcgtcctcg tccaccgctg
     1981 gtcgccagca tccacaacct cgtgctcgac gacgtcgccg gccggtcggc gccgctgctg
     2041 cgcctcctgg aggagcgcct ccccgggcgg gtcgacgcca ccatcgccgt gtcccgcgag
     2101 gtcgcacgcc gcttcgcggg ccgccccggc gccgaacgga tccacgtgat ccccccggcc
     2161 ggcccgccgc cggtgcctgc ccgatcggcg gagcaggtcc gtgccgagct cggcgtgggc
     2221 gaggatgagg acctggtggt caccgcggcg cgcctccacg cccagaaggg cctgcacgtg
     2281 ctgctcgacg ccgctgcagc gatccgcacg cagcgccccg gcctgcgctg gttcgtcttc
     2341 ggcgacggcc cgctgcgggg tgagctggag gaggcgatct cggcccgggg cctggagcgg
     2401 gtcgtgcacc tcgccggatc gcgtccggac gtcgacagcg agctggcagc tgccgacctg
     2461 gtcgtcgtca cctcccagtg ggagtccggt ccgctcgtgg tgctcgaggc gctcgccctc
     2521 ggacgcccgg tcgtgtccac cgacgtgggc ctcgtccccg aggtgatcga cccgggctcg
     2581 ggccgggtcg tgccggtggg cgacgcggtc cgcctggccg aggcggtcgt cgcggtcctg
     2641 gcgggttcgt ccgccccgga gggtgaggtg ccaggatcat cggcccggtt ccgtcccgaa
     2701 gccctcgcgg ctgccgtccg ggacgtctac gaagaggtca tgtccccccg atgaagccgc
     2761 tccggatcgc cgctgcactg gccgtcgtcg ccgtcgctgc cgtgctcctc gtcgtccccg
     2821 ggcgagggag cacctcgcgg gccgatgccg accgggtgct gatcgtgtcg atcccgaccc
     2881 tcacgtggga cctcgtcgcc gacgagcagc caccgaacct cgtgtcgctg ctggcccggt
     2941 ccgcggtggc gtccgccagc gtgcgcacgg tcggcatcga caccgacctc ggcgagggct
     3001 acgccaccat gggatcgggc aaccgcaccg cggtggtgac cgagtacgcc tcgctcgcct
     3061 tcccgccgga cgcgacgttc gagggcgaac cggtggccga cgtgtacgag cgtcgcttcg
     3121 gcacggcacc gggcgacgcc gaggtcgtcc agctcggtgc cgccgccatc cagcggcgca
     3181 acgacgatct gctctacggc gccgaggtcg gctcgctggg cgcggcgctg cgtgacgccg
     3241 gccgctccgc cgccgtggtc gccaacgccg agctcgtcgt ggaccccccg gtgtccgagc
     3301 tgagccgcca ggccgccctc gccgtgatgg acgacctcgg ccgcgtggca cggggtcggg
     3361 tcgacggcgg cctgctcgag gccgggcccg atctgcccgg tggggtggcg ctggacgccg
     3421 accaggtgct cgccgaggtc gagtcggcct gggacgacag cgacgtgctc atggtcgagc
     3481 tgagcgacct gctccgggcg gaggcgaccg agccggtcgt gacccccgag gtcgccgatg
     3541 cggtggccgc cgatgccctc gcccgcaccg acgacctcct cggtgcgctg ctcgagggcg
     3601 tggaccccga gcgcgacctg gtgctcgtgg tctccccggt ctccccggtg ggtcccgaca
     3661 acctcgtcgt cgccgccgcc gccggggccg gactcgagcc gggactggcc cgcagcggca
     3721 cctcgcggcg ggacggctac gtcacgctgc ccgacatcgg gcccacggtc ctcgacgccc
     3781 tgggcatcga caagcccggc gccatgagcg ccgcgccgat cgcgtcctcc ggcggcggtg
     3841 ctcccgacgc cggcaccttc cggggcctgg ccgacgacaa cgtccgagcg ctgttccgca
     3901 acagcctgtc cgggccgctg acggtgctgt tcatcatcag ccagatcctc acctacgcgg
     3961 tggcggcgta cgcgctgtcg agccaccgcc ggcagatggc cggcaccgtc gcgttcctgc
     4021 tcctggccgg gatggcgctc ccgttcgtgt cgttcctggc cggcgtcttc ccgtactggc
     4081 gactgagcgt cggtggggcg atcgccgcca tcttcgcggt gtccggcatc gtcgccgccg
     4141 tcgcccacct ggtgagcgcg cgcgccgtcg acagcggacg cgtccactcc cgcgccctcg
     4201 tgccgtcggt gctgatcatg tcggccacgg tggtgctgct ggtcggcgac atcgtcctcg
     4261 gtggaccgct ccagatcgac acggccttcg gctacggcgg cggcgcgatc gtcgccggtc
     4321 gactggccgg ctacggcaac ctcgcctggg cgctcatggt cgcggccctc atcatcgccg
     4381 ccaccggcat ctggggtcgc cgcgagctgt ccggcggtgt cggccgggac cggggtgatc
     4441 gccgcttcga cctcgccgtg gtcggcggcg tgttcgtgct ggcggtgctg gccgtcgggc
     4501 tgcctgccct cggcgcgaac gtcggcggca ccatctcggt ggtgtccggc ttggcgatca
     4561 cgctgctcgc cctcaccggt gcccggatcg acctccgtcg cctggtcggc gtcggcgtgc
     4621 tcaccgccct ggtcctcgcc gcgttcgggg cgctcgacct ccgccgagat ccccaggacc
     4681 agacccacct cggccggctc atcgaccaga cgctcggcga cgagggcatc gccgggctca
     4741 gcacggtgat cgagcgcaag atcaacgcca acctgaacat cctgctgtcc tcggtgtggt
     4801 cgctcgtgat ccccgccgcc ctcgccttcc tcgccttcct gatctggcgg ccgccccgct
     4861 tcctgcgcac ggtgttccag cggatccccg gcgtgcgggc ctgcgtgatc ggcgtcctcg
     4921 ccaccggcgt catcggcggg atcgtcaacg actcgggcat cgccatcccc gccgtcatgc
     4981 tgaccttgct gctgccccac gtcgcgtacc tcgtggtccg cacccacgac gcagcggtgg
     5041 tcgacgcgga acccgagacg tgaacgacgc ggtcgtcctg gccgtggccc tcggcctcgg
     5101 ggtcgccggc ggcgccctgg cctggccgat gctggcccag gtcttcgccg ccccgctctt
     5161 cctgcggacc aacgtccggg gtgccgaggt gccggtcgcg gtcggcatcg tcctgcccgt
     5221 ggtgctgctc gccgccggcg ccgccctcgt cgtgctcgac gccggcgacc tcgtgcaggt
     5281 gccccagggc gccctggccg tctccgtgct ggccgccgcc ggcttcggga tgctcggcct
     5341 cttcgacgac ctcgccgggg accgggcggc gcagggcttc cgggggcacc tgtcggcact
     5401 gcggtccggc cagctgacca ccggagcggt gaagctgttc ggcggcggtg ccctcgccgt
     5461 gctcgtcgcc gcgcccaccg cccgcgagcg cttctgggtc ctgctcgccg acgccgcgct
     5521 catcgcgctg tcggccaacc tcgccaacct gttcgaccgg gcccccggac gtctggcgaa
     5581 ggtgtcgctc ctcgccaccg tccccatcgc cgtcgtcgcc ggcatcgatc cggacctggc
     5641 cggcccggcg gtcgtgctcg gcgccctgct ggccctcctg gcgccggacc tgcgcgagcg
     5701 gctcatgctc ggggacacgg gcgcgaacgt cctcggcgcc accctggcgc tggccgtcgt
     5761 gctcaccacg gcgccctcga cccgggtcgt ggtcctggtc gtcgtggccg ccctgaacct
     5821 gctcagcgag cgggtcagct tcagccgggt gatccagcag gtccgcccgc tgcggtggtt
     5881 cgacggcatc ggccgcctcc ccccgacgac ctgaggcatg ctggccccgt gacgatgccc
     5941 gacttcctgg tgatcggagc agcccgctcc gcgacgacat ccctgcacta ctacctggac
     6001 cagcacccgg gcatcacgat gtcgcgcatc aaggagccga accacttcgc gttcgaccac
     6061 tccgtcgacc cgccgtcccc cctcatcgac ccggcgtcgc cgatcgtgac gaagtccgtg
     6121 cccgaccggc acgcgtacga gcagctgttc gcccacgccg ggcccggcga cgtcctcggg
     6181 gaggcatcgc cgctgtacct ctacgtccgg gaggccccgg agcagatccg gcgggcgctg
     6241 accgatccga agctggtggc ggtgctgcgc aacccggtcg accgggcgta cagccactgg
     6301 ctccacatcc accgtgaccg ccccgaggac gcggtcgccg cgttcgcccg tggcgccgag
     6361 cgggagatcg ccgaaccacc cgggtactcg ccctacgccg gtgccagcca cgtgctgcgg
     6421 atgggcctct acgacgagca gctgcaacgc cacctcgacg tcttcggccc cgacgccctc
     6481 ctcgtcctca gctacgacga ggtcacggtc cgccccgggg agtcgctgga gcggatctgc
     6541 gaccacctcg gcgtggcccg ccacgacttc gacaccggcg tgcagtacaa ccggtccggc
     6601 gtggccgggg gagcgctgcg ggcccgggtc gggcgggtcg tgcgagccgt ccaacccacc
     6661 gtcaagcggg tgctccccga caccgtggca gcccgcctcg gacggctgcg ggccgagcgc
     6721 gaccgccccg acgcggcccc gccgatcccg gccgacctgc gcgcccgtct cgtcgactgg
     6781 tactacccca gcgtcgagcg cctgcgggcc atggatgtgg tcgacatctc cggctgggac
     6841 gacttcacct gaccaggatc gtcccgagcg cgccggcgcg ggcctaggct gtggtcccgt
     6901 gacgaaacac atcttcgtga cgggtggcgt gacgagcagc ctgggcaagg gcctcaccgc
     6961 ctcctcgctg ggccgtctgc tgaaggcccg gggcctgcgg gtcacgctcc agaagctcga
     7021 cccgtacatc aacgtcgacc cggggaccat gaaccccttc gagcacggcg aggtgttcgt
     7081 caccgacgac gggggtgaga ccgacctgga tctcggccac tacgagcggt tcatcgacga
     7141 gagcctgacc aaggactcga acgccacgac cggctcgatc tactcggcgg tgctggccgc
     7201 cgagcggcgg ggggactacc tgggcaagac cgtccaggtc atcccgcaca tcaccgacga
     7261 gatcaagcgg cgcatcagcc ggctggccaa cgacgacgtc gacgtcgtca tcaccgaggt
     7321 cggcggcacc gtcggcgaca tcgagatcct cccgttcctc gaggcgatcc gccagttccg
     7381 cctcgacgtc ggtcggggca acgtctgcta cgtccacgtc acgctcgtgc cctacatcgg
     7441 cccgtccggc gagcagaaga ccaagcccac ccagcactcg gtcaccgagc tgcgcagccg
     7501 gggcatccag cccgacgcca tcgtggtccg ctcggccgac ccgatccccg acgacctgaa
     7561 gcacaagatc agtcgcctgt gcgacgtcca cgccgacggg gtcgtgaacg ccgccgacgc
     7621 cggcaacatc tacgagatcc ccctcgtgct ccacgaggag ggcctcgaca gcgtcgtgtg
     7681 cgacgtgctg cagatgaccg acctccccga ccccgacctt cgggagtggg aggccctggt
     7741 cgccaaggtg gaggccgcca cccgccccgt gcgcatcggc atcatcggca agtacgtggc
     7801 cctccaggac gcctacctgt cggtggtcga ggccacgaag cacggcggct tcgacatcgg
     7861 cgtcgacgtg gagttcgagt ggatccaggc cgaggaggtc gaggggctca tggccgacgg
     7921 ccgcctccgc gacctcgacg gcgtcgtcat ccccggcggc ttcggcgagc ggggcaccga
     7981 gggcaagatc gaggcggccc gctacacgcg cgagaacgag atcccgtgcc tggggctgtg
     8041 cctcggcctg cagatgatgg tcgtggacta cgcccgcaac gtcctcgaca tgccgggcgc
     8101 caactccacc gagatggagc cggccgccgt ggacccggtg atcacgatca tgccgagcca
     8161 gcacgacgtc accgacatgg gcggcaccat gcgcctcggc gcctacgtcg cccagctcac
     8221 gcccggctcc aaggtggccg aggcctacgg gaccaccgtg gtgtccgagc gccaccgcca
     8281 ccgctacgag ttcaacccgc ggtaccggtc gcgcttcgag gccagcgacc tgctgctctg
     8341 cggggagtcg ccagacggtc ggctggtcga gttcatcgag ctgcagggcc acccgttctg
     8401 ggtcggcacc caggcccacc ccgagctcaa gagccgcccc acccgcccgg caccgctgtt
     8461 ccgggccttc gcggccgcgg cgctggctcg ggccgagggc cgcaagccgc acctgttcga
     8521 cctggacgca ccggtcgagc agcccagcag cgtctcggcc gactaggtga cgtcgttccg
     8581 gctccagggt gaggaggccg tctacgacgg acacgtcatg cgcgtcgtcg tcggcacctt
     8641 cgaggggccc gacggggaca cgttcacccg tgacatcatc cgccaccccg gtgcggtggc
     8701 cgtgctgccg ctccacgagg acggcacggt gacgctggtg cgccagtacc gggcgccgct
     8761 cgacgagcac atcctggaga tccccgccgg catccgcgac gtcgaggacg aacccaccga
     8821 gcagaccgcc gtccgggagc tcgccgagga ggtcggcctg gaggccgagc acctcgagca
     8881 cctggtgtcg ttccacaacg cgccgggcat gtccgacgag gtcgtggagg tctacctcgc
     8941 caccgggctc cggtcggtgg acgccgacgc gcagggaccc gaggaggagg ccatgaccat
     9001 cgagcgccac cacctcgacg acctggccac gatgatcggc gacgggcagc tgacggacgc
     9061 gaagacggtg atcgccgtcg cgctcgtccg agcccgttga ccgcgtccga cgcggacgag
     9121 cagctgccgc tggagatcga ggagtacctg acgtggttgc ggctcgagcg cggccgggcc
     9181 cggtccaccg tggaggcgta ccgccgggac ctgcgggcct gggccgagca cctccaccac
     9241 ctcggacgag cggtgacgga ggtgaccgag gacgacctcg tcgcctgggt cggcgatctc
     9301 cgggagcggg ggctggcccc gtcgacccgg aagcgtcgcg tcgtggctgc ccgcaacctg
     9361 caccgctacc tggccgagga gggtcacgtc gacgacgacg tgggcgccga cgtcgctccg
     9421 ccgccggtgg cccgcggtct gccgaaggcg ctcaccgagg ccgaggtcga cgcgctgctc
     9481 tcgcgcgtcg tgggtgacga cccggtcgcc cgccgcgacc gggcgatcct ggagaccctg
     9541 tacggcacgg gcgtgcggat ctcggagctg gtctcgatgt ccttgcggga cctcgacctc
     9601 cacgacggct tcgtgcgggt cgtcggcaag ggggacaagg agcgagtggt gccggtcctg
     9661 ggcgccgccg ccgacgccct cggtgcctgg ctgacggtgg acggccgggg cgcgatgatc
     9721 ccggcccgct ggcgccgccg ggacgacgag gaggccgtct tcctcaacca gcggggcgga
     9781 cgactcggcc gccagggcac ctggctcgtc gtgaaggctc acgcagccga ggccggcctc
     9841 ggcgaccggc tcacccccca cgtgctgcgc cactcctgcg ccacccacct gctcgaccac
     9901 ggggccgaca tccgggccgt gcaggagctg ctcggccacg cctcgatcgc gaccacgcag
     9961 gtgtacacga aggtctcgac cgagcgcctc gtcgccgcct ggcgctccgc acacccgcgg
    10021 gccggcgcct gactcagtcg accgtcgccc agccctcggg gaggcggggg ccccgccagg
    10081 aggacggggg ttcccacctc gaccagcggt cgtcccacag ccgctcgccc acctccagcc
    10141 ggccgacaag gtcgtcacca agcgatcgga tccagtcgac caggccggcg gtgtagcgac
    10201 cctcggccac ccggtcgtcg agcacctcca ggtccttcac cacgagcgag ccatccgggt
    10261 acacgatcag gtcgagctcg aagtcctggg tgtcgtaccc gatcgacgtc cggacgaagt
    10321 cggcctgcag gttcacgtac cagcaggcga actcccggtc gggccccttc cagaagtgcc
    10381 acaccgccca cggatcgccg gggcgttgga ccatcaggca gccgtggccg ccccacgcct
    10441 gcttccgact ccaggggtgc agcccgtcgg gtgtgggcca cgggccgtcg acgaacccca
    10501 tctcagcccc ctcggcgatg taggtcacga ggtgctcggc gtcgtcgcgc accacgtaca
    10561 ccgggagcgc ctcccagggg cgtccgtacc atgggggtgg gggatcgggg tggaagccgg
    10621 gcgcgaggcc cagcacctcc cggcgcacga tgacctcacc cgtctcccac ccggccgcca
    10681 tggcgcgacg gtagggccga ccgaccggtc ggtgctgcgt cgtcgtctgt ggcccgtcag
    10741 acgggtgggg tgatgggtcc gtcgtcgtcg gggcggtgga tggtccaggt gccgtcgggg
    10801 tcgcgaaccg gtcggaagcc gtgttccttg gtgcggttgt ggaacccgca gtacgcatcg
    10861 gagttgtcgg tgttggtggg tccgccccgg caggccggat cgcggtggtc gacttgcagg
    10921 ttcttgcctc gtccgtcaca gcccggccac agacagccga gcccgccggg tcctcgcagc
    10981 agggcttgga ggaaggcggc gtcgcgggcg gagccggtga acaggcgttg gcggcgtccc
    11041 aggtcgatga cggtgccggc cgagtcgacc acgacccgac gcacatgacc gaccagggcc
    11101 gcggcgaccg cttcggccgg acggacctgg
//
LOCUS       DATISU010000367         1930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_773894, whole genome shotgun sequence.
ACCESSION   DATISU010000367 DATISU010000000
VERSION     DATISU010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773894"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VLR27_14605"
     CDS             <1..342
                     /locus_tag="VLR27_14605"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HSP04740.1"
                     /translation="AGDFIVILSASPQELVEAVAARLGAQRAVGTRAEIEGARYTGGL
                     DGPFCYGIGKVQRLEQDLGPGSLDAATAYADSVSDLPVLEACREPVAVNPDRQLHRLA
                     KARDWPVVRFG"
     gene            complement(390..1229)
                     /locus_tag="VLR27_14610"
     CDS             complement(390..1229)
                     /locus_tag="VLR27_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP04741.1"
                     /translation="MPVDQPTTTGTTAEGIEISLPAPAVPKPPTSHHLTRHVHVAPSA
                     RKVQAPMLQPTVLVVEDEESFIEALSVGLAREGFRVQVARDGIAAQTMFDAIQPDVVL
                     LDVLLPKVSGIDVCRELRKRSRVPIIMVTAKGSEIDAVVGLEVGADDYVTKPYRIREL
                     IARMRAVMRRQTGTDRTLPADAMQVGDVALDPERHEVRIRGSVTPMPLKEFELLHVLL
                     ANADRVMARGDLIDLVWGFDYVGDTKTLDVHVKRLRAKVETDPANPERIVTIRGLGYK
                     YAS"
     gene            complement(1229..>1930)
                     /gene="phoU"
                     /locus_tag="VLR27_14615"
     CDS             complement(1229..>1930)
                     /gene="phoU"
                     /locus_tag="VLR27_14615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HSP04742.1"
                     /translation="HEPLGTGYGQGGQRLRKSFHDELDQLRLQVELMGVKVDHNLERT
                     RRVLLDGDPDAAAAAVACDDEIDAMNVSLTERCYDLLARENPVASDLRFVVSVLRILS
                     ELERIGDLALRVVKLNPDRVLWAEHRPTFDILASMGETAIDAYRDALRAWSAQDLHLA
                     TDLATRVRLMDMHYEHLMAELLRLSGPHAVPIATSTLIAGRSLERIADHSAIIGARLR
                     YMLTGDPDHLTAEVR"
BASE COUNT          269 a          642 c          716 g          303 t
ORIGIN      
        1 gccggcgact tcatcgtcat cctgtcggcg agcccgcagg agctggtcga ggcggtcgca
       61 gcccggctcg gcgcgcagcg ggcggtcggc acccgcgccg agatcgaggg cgcgcgctac
      121 accggcggcc tcgacggacc gttctgctac ggcatcggca aggtgcagcg actggagcag
      181 gatctgggcc ctggttccct ggacgctgct acggcgtacg ccgactcggt ctcggacctg
      241 cccgtgctgg aggcgtgccg cgagccggtc gcggtcaacc cggaccgtca gctgcatcgg
      301 ctcgcgaagg cgagggactg gcccgtcgtg cgattcgggt gagcggctcg cgggtcccct
      361 gacagactgg cgagccactc ggcccgacct cagctggcgt acttgtagcc gagccctcgg
      421 atcgtgacga tgcgctcggg gttggccggg tcggtctcga ccttggcccg caggcgcttc
      481 acgtgcacat ccagcgtctt ggtgtcgccg acgtagtcga agccccagac gaggtcgatg
      541 aggtcgcccc gcgccatcac gcggtctgcg ttggcgagca gcacgtggag cagctcgaac
      601 tccttcaggg gcatgggcgt gaccgatccc cggatgcgga cctcgtgcct ctcgggatcg
      661 agggcgacgt cgccgacctg catggcatcg gcggggaggg ttcggtcggt gccggtctgg
      721 cgtcgcatga cggcgcgcat gcgggcgatg agctcgcgga tgcggtacgg cttggtgacg
      781 tagtcgtctg ccccgacctc gagccccacg acggcgtcga tctccgagcc cttcgccgtg
      841 accatgatga tcggcacccg agaccgcttc cgcagctcac ggcacacgtc gatgcccgac
      901 accttgggca gcagcacgtc gaggagcacc acgtcgggtt ggatcgcatc gaacatcgtc
      961 tgggcggcga tgccgtcgcg ggcgacctgc acccggaacc cttcgcgagc gagcccgacg
     1021 ctgagcgcct cgatgaagga ctcctcgtcc tccaccacca ggacggtcgg ttggagcatg
     1081 ggcgcttgga ccttccgggc cgagggggcg acgtggacgt ggcgggtcag gtggtggctc
     1141 gtcggcggct tcggcaccgc gggtgcgggg agcgagatct cgatcccttc ggccgtcgtg
     1201 ccggtcgtgg tcggctggtc gacgggcatc aacggacctc cgccgtgagg tggtcggggt
     1261 ctccggtgag catgtagcgc aggcgggcac cgatgatggc ggagtggtcc gcgatgcgct
     1321 ccagggaccg gccggcgatg agcgtggacg tggcgatcgg cacggcgtgc ggaccggaca
     1381 ggcggagcag ctcggccatg aggtgctcgt agtgcatgtc catcaaccgg acccgggtgg
     1441 ccaggtcggt ggcgaggtgg aggtcctggg ccgaccacgc ccgcagcgca tcgcggtacg
     1501 catcgatggc ggtctcgccc atcgacgcga ggatgtcgaa ggtcggccgg tgctcggccc
     1561 acagcacccg gtcggggttc agcttgacca cccgcagggc gaggtcgccg atccgctcca
     1621 gctcggacag gatccgcagc acggacacga cgaagcgcag gtcggaggcc accgggttct
     1681 cgcgggcgag caggtcgtag cagcgctcgg tgaggctcac gttcatcgcg tcgatctcgt
     1741 cgtcgcacgc aacggctgcc gcggcggcat cggggtcgcc gtcgaggagc acgcggcgcg
     1801 tccgctccag gttgtggtcg accttgacgc ccatcagctc gacctgcagc cgcagctggt
     1861 ccagctcgtc gtggaaggac ttgcgcaacc gttggccccc ctgcccgtac cccgtgccca
     1921 gtggctcgtg
//
LOCUS       DATISU010000368         3208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_820594, whole genome shotgun sequence.
ACCESSION   DATISU010000368 DATISU010000000
VERSION     DATISU010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820594"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VLR27_14620"
     CDS             complement(<1..699)
                     /locus_tag="VLR27_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04743.1"
                     /translation="MAQRFDELLGEHPGVATGPHRGVHGHEGAACVTAAQGVDEVVDG
                     AVGDRDAAGGGHLVEGGQRVAGRAPTGLDDVVDHVVVELEPGVGDHVPDVTGEHVGGQ
                     QVELEVLGAGPDGGQHLLRLGGGQHEHDVLGRLLQRLQQRVGRCRREHVHLVEHVHLA
                     VAARAHGDPAEQVAHGVHAVVGGGVELLEPVDVAPLLHRLAVGAGGVGLAVDGVLAVE
                     DLGQDAGRRRLAGAA"
     gene            953..1681
                     /locus_tag="VLR27_14625"
     CDS             953..1681
                     /locus_tag="VLR27_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04744.1"
                     /translation="MRWPPAATVEAAVAAAPTGPATPPRGAEAARARLAQHDATAPEA
                     PEPAAPAPAPAASRPSLGGVKRARQQEAASPRDVPAEAVTPTPAPEPESAPAAAPTPD
                     ADSGVPTLAELTAAWDDVLGALRGRAKALYKMGRLVEGDERTARFAVPNEPSLKNCSE
                     KLDEVLEALAARFGRPVPLDLVVDGDAATAPRPDPSSTTAAADEDIDDEVDLDELTDA
                     PDPGTGGIAQLKDAFPGAELLEDR"
     gene            1678..2097
                     /locus_tag="VLR27_14630"
     CDS             1678..2097
                     /locus_tag="VLR27_14630"
                     /inference="COORDINATES: protein motif:HMM:NF014616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HSP04745.1"
                     /translation="MSDQNPDVAGGGLPDLGGLLGGLGGGGGGGGLGDLFAQAQAAMS
                     ASQAAAEQQVEGTAGGGMVRIEATAGGEVLAVHIDPAVVDPDDVEGLEDLVLAALRDV
                     HAQAARVHAEAMGGFDPSSMLGGLLGGGADDEPDDEA"
     gene            2104..2703
                     /gene="recR"
                     /locus_tag="VLR27_14635"
     CDS             2104..2703
                     /gene="recR"
                     /locus_tag="VLR27_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HSP04746.1"
                     /translation="MHADAVQNLIDQLGRLPGVGPKSAQRIAYHLLKVPKEDALALAS
                     AVVTAKERVRFCEVCHNLSEAPRCQICADTRRDATLVCVVEEPRDIVSVERTGGFRGR
                     YHVLGGAINPMEGIGPDQLKVRELLGRIDSDAVVEVILCTNPNLEGDTTAMYLARLLK
                     PLGVRVTRIASGLPVGGDLEYADELTLGRALEGRRDVDA"
     gene            complement(2998..>3208)
                     /locus_tag="VLR27_14640"
     CDS             complement(2998..>3208)
                     /locus_tag="VLR27_14640"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HSP04747.1"
                     /translation="GVGFTVALFIASLAYDPGSLATDSAKIGILAGSVVAGLLGYGFL
                     RSGPSPDPGGDDSGRVREPAPALVD"
BASE COUNT          467 a         1225 c         1161 g          355 t
ORIGIN      
        1 cgcggcaccg gcaagacgtc gacggcccgc atcctggcca aggtcctcaa ctgcgagaac
       61 cccgtcgacg gcgagcccga caccacctgc gccaactgca aggcggtgga ggaggggcgc
      121 cacgtcgact ggctccagga gctcgacgcc gcctccaaca acggcgtgga cgccatgcgc
      181 gacctgctcg gccgggtctc catgggcacg agcggcaacc gcaaggtgta cgtgctcgac
      241 gaggtgcaca tgctctcgac ggcagcgtcc aacgcgctgc tgaagacgct ggaggagccg
      301 cccgagcacg tcgtgttcgt gctggccacc accgagccgg agaaggtgct gcccaccatc
      361 cggtcccgca cccagcactt cgagttcaac ctgctgcccg ccgacgtgct cgccggtcac
      421 gtccggcacg tgatcgccga cgccgggctc gagctcgacg acgacgtggt cgaccacgtc
      481 gtccaggccg gtcggggctc ggcccgcgac acgctgtccg ccctcgacca ggtggccgcc
      541 gccggcggcg tctcggtcgc cgacagctcc gtcgacgacc tcgtcgacgc cctgggccgc
      601 cgtgacgcag gcggcgccct cgtggccgtg gacgccgcgg tgcgggccgg tcgcgacccc
      661 cgggtgctcg ccgagcagct catcgaatcg ctgcgccacg ccttcctcgt cgccatgggc
      721 gcacccgccg gtgggctcac cgaccgggcc caggcccggg cgcgcgacgt cagccaggcg
      781 ctgggaccgg cctcgctcac ccgggcgctg gaggccgtcg gcacctccgt ggtggagatg
      841 cgccacgccc ccgacccccg catccccctc gaggtcgcgc tggtgcagat cgcccgaccc
      901 gaggcagcgg gtgacctcga cgccctggtg cgacgcatcg agcagctcga acgtgcgctg
      961 gcctccggcg gccaccgtcg aggcggcggt cgccgccgcc ccgaccggtc cggccacacc
     1021 accccgcggc gccgaagcgg cccgggcccg cctcgcccag cacgacgcca cggctcccga
     1081 ggcaccggag ccggcggcgc cggcgccggc accggcggcc tcccgcccga gcctgggcgg
     1141 ggtcaagcga gcccgccagc aggaggcagc gtcaccgcgc gacgtcccgg ccgaagcggt
     1201 gaccccgacg ccggcgcccg agccggagtc ggcacccgcg gccgcaccca cgcccgacgc
     1261 cgacagcggt gtgccaaccc tggccgagct gacggccgcc tgggacgacg tgctcggcgc
     1321 gctgcgcggg cgggccaagg cgctctacaa gatgggccgc ctcgtggagg gcgacgagcg
     1381 cacggcgcgc ttcgcggtgc ccaacgagcc cagcctcaag aactgctcgg agaagctgga
     1441 cgaggtgctc gaggcgctcg ccgcccgctt cggccggccg gtgccgctcg acctcgtggt
     1501 cgacggcgat gccgccaccg caccccgacc cgacccgtcg agcaccaccg cagccgccga
     1561 cgaggacatc gacgacgagg tcgacctcga cgagctgacc gacgcacccg accccggcac
     1621 gggcggcatc gcccagctga aggacgcctt ccccggcgcc gagctcctgg aggaccgatg
     1681 agcgaccaga accccgacgt ggccggtggc ggcctccccg acctcggcgg gctgctcggc
     1741 ggcctcggcg gaggcggtgg tggcggcggt ctcggcgacc tgttcgccca ggcgcaggcg
     1801 gcgatgagcg cgagccaggc cgcagcggag cagcaggtcg agggcaccgc cggcggcggc
     1861 atggtccgga tcgaggcgac cgccggtggc gaggtcctcg ccgtgcacat cgacccggcc
     1921 gtggtggatc ccgacgacgt cgagggcctc gaggacctgg tgctcgccgc cctgcgcgac
     1981 gtgcacgccc aggctgctcg cgtgcacgcc gaggcgatgg gtgggttcga cccgtcctcc
     2041 atgctcggtg ggctcctcgg cggcggagcc gacgacgaac ccgacgacga ggcctgagcg
     2101 cgggtgcacg ccgacgcggt ccagaacctg atcgaccagc tcgggcggct gcccggcgtg
     2161 gggcccaagt ccgcgcagcg catcgcctac cacctgctga aggtgcccaa ggaggacgcc
     2221 ctggcgctcg cctcggccgt ggtcaccgcc aaggagcggg tccggttctg cgaggtgtgc
     2281 cacaacctgt cggaggcgcc gcgctgccag atctgcgccg acacccgccg cgacgccacc
     2341 ctcgtctgcg tcgtcgagga gccccgggac atcgtgtcgg tcgagcgcac cggcgggttc
     2401 cgcggccgct accacgtgct cggcggggcc atcaacccga tggagggcat cggtcccgac
     2461 cagctcaagg tgcgcgagct gctgggccgc atcgacagcg acgccgtcgt cgaggtcatc
     2521 ttgtgcacca acccgaacct cgagggcgac acgaccgcca tgtacctggc ccggctgctg
     2581 aagccgctcg gcgtgcgggt gacccgcatc gccagcggcc tgccggtggg cggcgacctg
     2641 gagtacgcgg acgagctcac cctcggccgg gccctcgagg gccggcgcga cgtcgacgcc
     2701 tgacgcaccg ccgacccgga gcccggacgc gacaccggcc cgacccagag ggagagggga
     2761 tcgggccggc gaggcagttc caacccgggc atcgaccgct ggacggaggc gggcgggcgg
     2821 tgcgaaccgc ccagcacccg atgcccgggg agggtgtgag gtcggtgacc gggccgaagc
     2881 cctgtgtcga tccgacgttg cagtcacatt acaacgatga cgcacccgtg acaagccctg
     2941 tccgaaaagt ccgggaaaga tcccctccca tgacggatgg atgacgagaa gcggggttca
     3001 gtcgaccagt gccggcgccg gttcgcggac ccggccggag tcgtcgccgc cgggatccgg
     3061 gctcgggccg gaccgcagga agccgtagcc gagcaggccg gcgaccacgc tgccggcgag
     3121 gatgccgatc ttggccgagt ccgtcgccag gctccccggg tcgtaggcga gcgaggcgat
     3181 gaacagcgcg acggtgaacc cgacgccc
//
LOCUS       DATISU010000369         4539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_634287, whole genome shotgun sequence.
ACCESSION   DATISU010000369 DATISU010000000
VERSION     DATISU010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634287"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      10..88
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      95..189
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            221..3079
                     /gene="gcvP"
                     /locus_tag="VLR27_14645"
     CDS             221..3079
                     /gene="gcvP"
                     /locus_tag="VLR27_14645"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HSP04748.1"
                     /translation="MTTTGPTTSSGFRSRHIGPRGDDIATMLDVVGAGSLDELIDQAV
                     PSSIRSAEALDLPAAASEEEALAELRALAERNVVRTAMIGLGYHGTHTPPVIRRNVLE
                     SPAWYTAYTPYQPEISQGRLEALLNFQTVITELTGTELASSSLLDEGTAAAEAMAMCG
                     RLVKGDRPRFVVAADTFPQTIAVLQARAEAIGIAVEVVADVAATDLADACGVLVQYPA
                     ASGAVVDPSPAIEAAHDAGALAVVATDLLACTLLTPPGELGADIVVGSSQRFGVPLGF
                     GGPHAGFMGCREAYKRTLPGRLVGVSVDAAGRPATRLALQTREQHIRREKATSNICTA
                     QVLLAVIASMYAVYHGPEGLRAIAARVHGLTSAFATRLEQAGRTVRNEAWFDTLTVEV
                     PGEASDVVARAAEEGIDIRLVDADTVSLAFDETCTEATSDRLAVAFGGATGELPEREG
                     VAVPEGLVRTSDFLTHPTFHSYRSETEMLRYLRRLADKDVALDRAMIPLGSCTMKLNA
                     TAEMEPISWPGFANLHPFVPATSSEGYRTLIDQLEAWLVEVTGYDAVSFQPNAGSQGE
                     LAGLLAIRSFHDANGDTDRRVCLIPSSAHGTNAASAVMAGMRVVVVACDDDGNVDVDD
                     LKAKVTDHADTLAGLMVTYPSTHGVFETRIREICDLVHDHGGQVYLDGANLNALVGVA
                     KPGEFGADVSHLNLHKTFCIPHGGGGPGVGPVAVRSHLAPYLPNHPLDDAAGPATGPG
                     PVSAAPYGSAGILPISWAYVRMMGGPGLTEATHLAILNANYIAARLGERYPVLYAGEH
                     GRVAHECIVDLRDITRRTGVTVDDVAKRLIDYGFHAPTMSFPVAGTLMIEPTESESLA
                     EIDRFCDAMLAIADEIAEVESGAVAHDDSALAHSPHTAEDLLVANWDRPYSREQAAYP
                     VDGLRQDKYWPPVSRIDGAAGDRNLVCACPPPEAFEQG"
     gene            3129..>4539
                     /locus_tag="VLR27_14650"
     CDS             3129..>4539
                     /locus_tag="VLR27_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04749.1"
                     /translation="MATRIDIELTSRQDEGKTFTWRAAGARQPKGAAEASLFPEGAEV
                     GDEFRVETEMDVDGIRVTQVVPAKGKEPKKVETLELIARDDEPLVTQVLAEKGRGRGD
                     RGDRGPRRGGGGRDGGRGRKEGDRGPRGEGGDRGPRGEGGRRGGNDRPRTPAKPKLPP
                     KPKPPRLKAGKTHRNALIDSLPDEQKAVAQELLRGGLQAVRTGLEKQNEQRKVEGQDP
                     IDEGPIVTLAEELLVKVRAAEWRDRAEAALEQSETVDLRDLRTVVTAADGAAKDDETR
                     ALAAKVRTALNERLDADQQAWVAEVVEMLDDDRVVRALHLSSRPPKAGAPLPAPVALR
                     LVEAANASMSSETSADRWSYILEALAHSPVRRQVVPVSLPNTVSDELKATIARHGSRL
                     PEIAQIFGVEPDPKAGRDAARRKRRSGGKPKKDAKPGEAKGERKADKPKGDKPKADEP
                     KGDEPKAEEPKRDEPTAEEP"
BASE COUNT          698 a         1770 c         1544 g          527 t
ORIGIN      
        1 gacccccgac gggagagtcc cgccccaccg ccagtgggga gggcgccgaa ggagcaaccg
       61 ccccggaacc tctcaggcca ccggaccgcc gggacgaggc acctctggag agcagcctgc
      121 gggctcaccg aaggagcaag cccggcctcg ctgggcgaat ctctcaggtc acggaacaga
      181 aggggctcca cgccgacgcg caccagggag ctcccccacc atgaccacca ccggaccgac
      241 cacgagctcg ggcttccgca gccgccacat cggcccccgt ggcgacgaca tcgccacgat
      301 gctcgacgtc gtcggcgccg gctccctcga cgagctgatc gaccaggcgg tgccgtcctc
      361 gatccgctcc gccgaggctc ttgacctgcc ggcagccgca tcggaggagg aggccctcgc
      421 cgagctgcgg gccctggccg agcgcaacgt cgtgcgcacc gcgatgatcg gcctcggcta
      481 ccacggcacc cacacgccgc cggtcatccg gcgcaacgtg ctcgagagcc ccgcctggta
      541 caccgcctac accccgtacc agcccgagat ctcccagggc cgcctcgagg cgctgctcaa
      601 cttccagacc gtcatcaccg agctcaccgg caccgagctg gccagctcgt cgctgctgga
      661 cgagggcacc gcggccgccg aggccatggc gatgtgcgga cgcctggtga agggcgaccg
      721 gccccgcttc gtggtcgccg ccgacacctt cccccagacc atcgccgtgc tgcaggcccg
      781 cgccgaggcg atcggcatcg ccgtcgaggt cgtggcggac gtggccgcca ccgacctggc
      841 cgacgcctgc ggcgtgctcg tccagtaccc ggcggcatcg ggcgcggtgg tcgacccctc
      901 ccccgccatc gaggccgccc acgacgccgg cgccctcgcc gtggtggcca ccgacctgct
      961 ggcctgcacg ctcctgaccc cgcccggcga gctcggcgcc gacatcgtcg tcggctcctc
     1021 gcagcgcttc ggcgtgcccc tcggcttcgg cggcccccac gccgggttca tgggctgccg
     1081 ggaggcgtac aagcggaccc tgcccggccg tctcgtcggc gtgtccgtcg acgctgccgg
     1141 ccggcccgcc acccgactcg ccctccagac ccgcgagcag cacatccgac gggagaaggc
     1201 gacctccaac atctgcaccg cccaggtcct gctggcggtc atcgcctcga tgtacgccgt
     1261 gtaccacggg cccgaggggc tgcgggccat cgccgcgcgg gtccacggac tgaccagtgc
     1321 cttcgccacc cggctggagc aggccggccg gaccgtccgc aacgaggcct ggttcgacac
     1381 gctcaccgtc gaggtcccgg gcgaggcctc cgacgtcgtc gcgcgggccg cagaggaggg
     1441 catcgacatc cgcctggtcg acgccgacac cgtgtcgctg gcgttcgacg agacgtgcac
     1501 cgaggccacc tcggatcgcc ttgccgtggc gttcggtggc gcgacagggg aactgccaga
     1561 acgggagggg gtggcggtgc ccgaggggct cgtgcgcacc agcgacttcc tcacccaccc
     1621 gacgttccac tcgtaccggt ccgagaccga gatgctgcgc tacctgcgcc ggctggcgga
     1681 caaggacgtc gccctggacc gggccatgat cccgctcggg tcctgcacga tgaagctcaa
     1741 cgccacggcc gagatggagc ccatctcctg gcccgggttc gccaacctcc accccttcgt
     1801 cccggccacg agcagcgagg gctaccgcac gctcatcgac cagctcgagg cgtggctggt
     1861 ggaggtcacc ggctacgacg cggtgagctt ccagcccaac gccgggtccc agggtgagct
     1921 ggccggcctg ctcgccatcc gctccttcca cgacgccaac ggcgacaccg accgtcgggt
     1981 ctgcctcatc ccgtcctcgg cgcacggcac caacgcggcc tcagcggtca tggccggcat
     2041 gcgggtcgtc gtggtggcct gcgacgacga cggcaacgtc gacgtcgacg acctgaaggc
     2101 caaggtcacc gaccacgccg acaccctcgc cgggctgatg gtgacgtacc cctccacgca
     2161 cggcgtgttc gagacccgga tccgcgagat ctgcgacctg gtccacgacc acggcggcca
     2221 ggtgtacctc gacggcgcca acctcaacgc cctggtcggc gtggccaagc cgggcgagtt
     2281 cggcgccgac gtgagccacc tgaacctgca caagacgttc tgcatccccc acggcggcgg
     2341 agggcccggc gtcggaccgg tggccgtgcg atcgcacctc gccccgtacc tgcccaacca
     2401 ccccctcgac gacgccgccg gcccggccac cggtcccggt cccgtgtcgg ccgccccgta
     2461 cggctcggcc ggcatcctgc cgatctcgtg ggcctacgtg cggatgatgg gcggccccgg
     2521 cctcaccgag gccacccacc tggcgatcct gaacgccaac tacatcgccg cccgcctcgg
     2581 cgagcgctac ccggtgctct acgccggcga gcacggccgg gtggcccacg agtgcatcgt
     2641 ggacctgcgc gacatcaccc gccgcacggg cgtcaccgtc gacgacgtgg ccaagcgcct
     2701 catcgactac gggttccacg cccccacgat gagcttcccg gtcgccggca ccctgatgat
     2761 cgagccgacc gagtcggagt ccctcgccga gatcgaccgc ttctgcgacg ccatgctcgc
     2821 catcgcggac gagatcgccg aggtcgagtc cggtgcggtc gcgcacgacg actcggcgct
     2881 ggcgcactcc ccccacaccg ccgaggacct cctggtggcg aactgggacc gcccgtacag
     2941 ccgcgagcag gccgcctacc cggtcgacgg gctgcgccag gacaagtact ggccgccggt
     3001 gagccggatc gacggggccg cgggcgaccg caacctggtg tgcgcctgcc cgccgccgga
     3061 ggccttcgag cagggctgag agggcaatcg tcggtgccgg gacggctcgg ttagcctggc
     3121 aacccgctat ggccacccgg atcgacatcg aactcaccag ccgccaggac gaggggaaga
     3181 ccttcacctg gcgggccgcc ggggcccgtc agcccaaggg tgcggccgag gcgtcgctgt
     3241 tccccgaggg cgccgaggtc ggtgacgagt tccgcgtcga gaccgagatg gacgtcgacg
     3301 ggatccgggt cacccaggtc gtgccggcca agggcaagga gcccaagaag gtcgagaccc
     3361 tcgagctgat cgcccgcgac gacgagccgc tggtgaccca ggtgctggcc gagaagggcc
     3421 ggggtcgagg cgatcgcggc gaccgcggcc cccgccgtgg tggcggtggt cgcgacggtg
     3481 gtcgtggccg caaggaaggc gaccgtggtc cccgtggcga gggcggcgat cgcggccccc
     3541 gtggcgaggg cggacgtcgg ggcggcaacg accggccccg caccccggcc aagcccaagc
     3601 tccccccgaa gccgaagccg ccgcgcctga aggccggcaa gacccatcgc aacgccctca
     3661 tcgactccct gcccgacgag cagaaggccg tcgcccagga gctgctgcgc ggcggcctgc
     3721 aggccgtgcg caccgggctc gagaagcaga acgagcagcg caaggtcgag ggccaggacc
     3781 ccatcgacga ggggcccatc gtcaccctgg ccgaggagct gctggtgaag gtccgcgccg
     3841 ccgagtggcg cgaccgggcc gaggccgccc tcgagcagtc cgagaccgtc gacctgcgcg
     3901 acctgcgcac cgtcgtcacg gccgccgacg gcgccgccaa ggacgacgag acgcgtgcgc
     3961 tcgccgccaa ggtgcgcacc gccctgaacg agcgcctcga cgccgaccag caggcctggg
     4021 tggccgaggt cgtcgagatg ctcgacgacg atcgcgtcgt ccgggccctg cacctgtcgt
     4081 cccgcccgcc caaggccggc gcaccgctgc ccgcccccgt cgcgctccgc ctcgtcgagg
     4141 ccgccaacgc gtcgatgagc agcgagacct ccgccgaccg gtggagctac atcctcgagg
     4201 ccctcgccca ctcccccgtc cggcgccagg tcgtgccggt gtcgctgccg aacacggtgt
     4261 cggacgagct gaaggccacc atcgcccgcc acggcagccg cctgcccgag atcgcgcaga
     4321 tcttcggcgt cgagcccgac ccgaaggccg gccgcgacgc ggcccggcgc aagcgccgca
     4381 gcggcggcaa gcccaagaag gacgccaagc ccggcgaggc caagggcgag cgcaaggctg
     4441 acaagcccaa gggtgacaag cccaaggccg acgagcccaa gggtgacgag ccgaaggctg
     4501 aggagcccaa gcgcgacgag cccacggctg aggagccca
//
LOCUS       DATISU010000370         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_495424, whole genome shotgun sequence.
ACCESSION   DATISU010000370 DATISU010000000
VERSION     DATISU010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495424"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..300)
                     /locus_tag="VLR27_14655"
     CDS             complement(16..300)
                     /locus_tag="VLR27_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04750.1"
                     /translation="MTDTRTAWGEVGDHLSALLLKLKLHAEEELSDDEVREKCGLDRV
                     SAVLEETAEAMSDAYEDEAVRADARELARSFVDAVDTTFREAQTRMRTGG"
     gene            467..598
                     /locus_tag="VLR27_14660"
     CDS             467..598
                     /locus_tag="VLR27_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04751.1"
                     /translation="MVPAGEQEQDDDGAYRLTELGEPLRAALQPLDGWAQDWARRLD"
     gene            654..1070
                     /locus_tag="VLR27_14665"
     CDS             654..1070
                     /locus_tag="VLR27_14665"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSP04752.1"
                     /translation="MQPRDGQEPTTALEVVTAIYDAFARKDLEAVLALTSPDIVVTQD
                     TALPWGGRFEGHDGLGRFVMALLGSIDSAVTVEAIFQAGDRVVQYGRTAGTVRSTGAS
                     FDIPECHLWRVEDGVAVEAQYFIDSAAMLEALRAPG"
     gene            1125..1823
                     /locus_tag="VLR27_14670"
     CDS             1125..1823
                     /locus_tag="VLR27_14670"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP04753.1"
                     /translation="MEDDADLLAEQRAYYRARAPEYDEWWQRRGRYDRGEEERAEWDR
                     QVAVLDEALADFGATGRVLELAGGTGWWTERLARTADELTVVDASAETLEHNRTRVGR
                     DDVTYVVADVFEWRPTQQYDVVFFSFWLSHVPRPRFGEFWSLVHDCLAPDGRAFLIDS
                     REDQPSSSIRDPYVLEYQEDLHRRHLGDGSEWRVVKVMYEPDELNQLLEAEGWRPQID
                     GTRWFLYGAAEPAS"
     gene            complement(1831..>2100)
                     /locus_tag="VLR27_14675"
     CDS             complement(1831..>2100)
                     /locus_tag="VLR27_14675"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440277.1"
                     /note="catalyzes the cleavage of carbon-halogen bonds in
                     aliphatic compounds forming a primary alcohol and a
                     halide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HSP04754.1"
                     /translation="LQWPRELPIEGEPADVTEIAEAYTEWLSTSDVPKLFVNAEPGAL
                     LTGEQRERVRAFPNQTEVTVAGSHFVQEDSPHEIGRAIADWLPIR"
BASE COUNT          291 a          720 c          769 g          320 t
ORIGIN      
        1 ccctgggcgc ggcggtcagc cgccggtgcg catgcgggtc tgcgcctccc tgaacgtcgt
       61 gtcgacggcg tcgacgaagg accgggcgag ctccctggcg tcggcccgga cggcttcgtc
      121 ctcgtaggca tcggacatgg cctcggcggt ctcctcgagc accgcgctca cccggtcgag
      181 gccgcacttc tcgcggacct cgtcgtcgga gagctcctcc tcggcgtgca gcttcagctt
      241 caggaggagg gcggagaggt ggtcgccgac ctcgccccat gcggttcggg tgtcagtcat
      301 gggtccacgg tgcgctccgg tcggcgcagg tggaagggtc cttcgtccct ggagggctgg
      361 tgcacctcgc cgtcgttcag gtggcgcaga cgatctggtg atcggcaaac cgaggcgcgc
      421 cgggctcgag cggtacgccg atgccacggg ccacgaagct gatggcgtgg taccagccgg
      481 tgagcaggag caggacgacg acggcgccta ccggctgacc gagctcgggg agccgctgcg
      541 ggctgcgctg caaccgctcg acggttgggc gcaggactgg gcccgccgcc tggactgagc
      601 cgtcgctcgg agctctgacg gcacgctcgg agcggctgga gtaggcattc ctcgtgcaac
      661 cgcgagacgg gcaggagccg accaccgcac tcgaggtcgt caccgccatc tacgacgcct
      721 tcgcccgcaa ggacctcgag gcggtgctgg cactgaccag ccccgacatc gtcgtcacgc
      781 aggacacggc gctgccttgg ggcggccgct tcgaaggcca cgatggcctg ggccgcttcg
      841 tcatggcgct gctcggctcg atcgactcgg ccgtcaccgt cgaagccatc ttccaggccg
      901 gcgaccgggt ggtgcagtac gggcgcaccg ccggcacggt tcgctcgacc ggcgcgagct
      961 tcgacatccc cgagtgccac ctgtggcggg tcgaggacgg cgtcgccgta gaggcgcagt
     1021 acttcatcga ctcggccgcg atgctcgagg ccctgcgcgc cccgggctga gccggcggtt
     1081 tacccctgac aggatcctgt cgtcgccggt tacggtcagc gccggtggag gacgacgccg
     1141 acctgctggc tgagcaacgg gcgtactacc gggcccgggc gccggagtac gacgagtggt
     1201 ggcagcggcg cggtcgctac gaccgcggcg aggaggagcg ggccgaatgg gaccgtcagg
     1261 tcgccgtgct cgacgaggcg ctggccgact tcggcgccac cggtcgggtg ctggagctgg
     1321 ccggcggcac cggctggtgg accgagcgcc tggcccgcac cgcggacgag ctcaccgtgg
     1381 tcgacgcatc tgccgagacg ctcgagcaca accggacccg ggtcggtcgt gatgacgtca
     1441 cctacgtcgt ggccgacgtc ttcgagtggc gcccgaccca gcagtacgac gtcgtgttct
     1501 tctcgttctg gctgtcgcac gtgccgaggc cgaggttcgg cgagttctgg tcgctggtgc
     1561 acgactgcct cgcacccgat ggccgggcgt tcctcatcga cagtcgggag gaccagccgt
     1621 cgtcgtcgat ccgggacccc tacgtcctcg agtaccagga ggacctccac cgacggcacc
     1681 tgggcgacgg ctccgagtgg cgggtggtga aggtcatgta cgaacccgac gagctgaacc
     1741 agctgctcga ggccgagggc tggcggccgc agatcgacgg cacccgctgg ttcctctacg
     1801 gggccgccga gccggccagc tgatcgccga tcagcggatg ggcagccagt cggcgatggc
     1861 acgcccgatc tcgtgcgggc tgtcctcctg gacgaagtgg ctgccggcca ccgtcacctc
     1921 ggtctggttg gggaaggcgc gcacccgctc ccgctgctcc ccggtgagca gggcaccggg
     1981 ctcggcgttg acgaacagct tcgggacgtc ggacgtggac agccactcgg tgtaggcctc
     2041 ggcgatctcg gtgacgtcgg cgggctcacc ctcgatcggc agctcccgcg gccactgcaa
//
LOCUS       DATISU010000371         4013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_169969, whole genome shotgun sequence.
ACCESSION   DATISU010000371 DATISU010000000
VERSION     DATISU010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169969"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VLR27_14680"
     CDS             <1..402
                     /locus_tag="VLR27_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-hexose 4-ketoreductase"
                     /protein_id="HSP04755.1"
                     /translation="RPEFLNRIDETIVFHELSKDEVIQIVDMFVAKLAIQMEAQGMGI
                     ELTKAAKLYVADKGYDPTLGARPLRRAIQRFVEDPLSEKLLYKQFRAGEIVTVDVEPD
                     PETNEDVIVFRSEEGFVPPSVEELEAAATPE"
     gene            complement(488..1021)
                     /locus_tag="VLR27_14685"
     CDS             complement(488..1021)
                     /locus_tag="VLR27_14685"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HSP04756.1"
                     /translation="MALDPQQAVRDIALAFPEAVEKQAGDHPTYRVRDKIFVSSGVDD
                     DGRATITVKAAHGEHGTLLARGEPFFSPKYVGARGWVGMRLDDETDWRQVHELIIDSY
                     REIAPGPLALGIGVRSAERSKAAAAFVAVGGVIGELVEGKPPKDQQVAEDVQEDADDG
                     PRIALDPDDPGSSTVQL"
     gene            1121..1492
                     /locus_tag="VLR27_14690"
     CDS             1121..1492
                     /locus_tag="VLR27_14690"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSP04757.1"
                     /translation="MYSRILVGTDGSSTADKAVARAVEVARSAGASLTIMTASSEAKG
                     RPVVDRAAADHADAGVEIDTLVVDKDPVGALIDTAEQGYDLVVVGNKGMTGVSRFFKV
                     GAVPNKLSHHLPTSMLVVRTT"
     gene            1499..2263
                     /locus_tag="VLR27_14695"
     CDS             1499..2263
                     /locus_tag="VLR27_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04758.1"
                     /translation="MRRAGLALLVLLSLAGCEVRTELNVTVDEDGSGDVELAVALDED
                     AMSRRPDLLDELDLSDLTDTGWEVTGPAEEADGSTWIRASHDFGTPEELGTLVDDVAG
                     EQGPFRDFRLTREDAFAERRYDFTGTVDFTAGAGALAEDPELAEALEAEPIELLEERL
                     GAAIDEMVEVQVAVRMPGTVDSNALTQASNGAVWRPSVVGREVVELQATGTVSRSDRL
                     VWAGVAAVATFALVLYLLIRAAAWRRSRRRSTPAGS"
     gene            2305..3684
                     /gene="radA"
                     /locus_tag="VLR27_14700"
     CDS             2305..3684
                     /gene="radA"
                     /locus_tag="VLR27_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HSP04759.1"
                     /translation="MTTTRPRSSRPRRSTFRCSSCGAGAPVWAGRCTGCDEWNTLVEE
                     RTSAVTRTVGAPATPARPIADITDQDVAPMPTGVPELDRVLGGGLVPGSVTLVGGEPG
                     IGKSTLLLQTASAMASGGRSALYLTAEESAPQVRGRAQRLGALADQLWLAADTALPDV
                     LGHLDAVQPSVLIVDSIQTVHDPELASAPGAVGQVRECAQRLVTEARARGVAVVLVGH
                     VTKDGGLAGPRVLEHLVDTVLSFEGDRHHALRLLRAVKHRFGGTGELGVFEMSDAGLV
                     GVDDPSALFLADRRTGEAGSVVIPVLDGGRPLLVEVQALLAPTTIPTPRRQGQGVESS
                     RLGMLLAVLDQRVGVSAANLDVWVTVAGGVRATEPAADLGVALAVVSAMTGVPVPGDL
                     VACGEVGLAGELRQVGRIDRRLAEAARLGFRRVLVPTTAPEPPAGCQAIRARGLREAI
                     AAVGLDRSG"
     gene            3694..>4013
                     /locus_tag="VLR27_14705"
     CDS             3694..>4013
                     /locus_tag="VLR27_14705"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HSP04760.1"
                     /translation="MPSRTSPAMLEALAFVAPGAPLREGLDRILQAEMGALIVVGDGP
                     DVLNICSGGFLLDAAFSPQRLSELAKMDGAIILAPDATRIARANVHLVPNPNVPTSET
                     GTRHR"
BASE COUNT          521 a         1506 c         1433 g          553 t
ORIGIN      
        1 cggcccgagt tcctgaaccg catcgacgag acgatcgtgt tccacgagct cagcaaggac
       61 gaggtcatcc agatcgtcga catgttcgtc gccaagctgg ccatccagat ggaggcccag
      121 ggcatgggca tcgagctcac caaggccgcc aagctgtacg tcgccgacaa gggctacgac
      181 cccacgctcg gcgctcgtcc gctgcgtcgg gccatccagc gcttcgtcga ggatccgctg
      241 tcggagaagc tgctctacaa gcagttccgc gccggcgaga tcgtcaccgt cgacgtcgag
      301 cccgaccccg agacgaacga ggacgtcatc gtgttccgct ccgaggaggg cttcgtgccc
      361 ccgtcggtcg aggagctcga agcagccgcc acccccgagt agggggagcg aagcaacccc
      421 gcaacgaact ggcagccctc ccgtcacgcc gaaggtgtga cgagagggct gccagttcct
      481 cgcgcggtca gagctggacg gtggaggacc cgggatcgtc cgggtcgagc gcgatgcgcg
      541 gtccgtcgtc ggcgtcctcc tggacgtcct cggccacctg ctggtccttg ggcggcttgc
      601 cctcgaccag ctcgccgatg acgccgccga cggcgacgaa cgcggcggcc gccttgctgc
      661 gctcggcgga gcggacgccg atgccgaggg ccagcggtcc cggggcgatc tctcggtagg
      721 agtcgatgat gagctcgtgg acctgccgcc agtcggtctc gtcgtccagc cgcatgccga
      781 cccagccccg agcgccgacg tacttcggcg agaagaacgg ctccccccgg gcgagcaggg
      841 tcccgtgctc gccgtgcgcg gccttgacgg tgatggtggc ccggccgtcg tcgtcgacgc
      901 cggagctcac gaagatcttg tcccgcaccc ggtaggtcgg gtggtcgccc gcctgcttct
      961 ccaccgcctc ggggaaggcg agggcgatgt cgcggaccgc ctgctgggga tcgagggcca
     1021 tgacgaccat cctcacagga aaatcccgtg gatccgcacc gccggtggtg gacgatccac
     1081 gacatttcgc tcggaggcgg tccggcctac gctgcccggc gtgtactcgc gcatcctggt
     1141 cggcaccgac ggatcctcca cggcggacaa ggccgtcgcc cgggcggtcg aggtcgcccg
     1201 ctccgccggc gccagcctca cgatcatgac ggcgtcgtcg gaggccaagg gccgccccgt
     1261 cgtggaccgg gccgccgccg accacgccga tgccggcgtc gagatcgaca ccctggtggt
     1321 cgacaaggac ccggtcggcg cgctcatcga cacggccgag cagggctacg acctcgtcgt
     1381 ggtcgggaac aagggcatga ccggcgtcag tcggttcttc aaggtcgggg cggtgccgaa
     1441 caagctgtcc caccacctgc ccaccagcat gctcgtcgtc cggacgacct gagtgcggtt
     1501 gcgccgcgcc gggctggccc tgctggtcct gttgagcctc gccggctgcg aggtccgcac
     1561 cgagctcaac gtgacggtgg acgaggacgg ctcgggtgac gtggagctgg ccgtcgcgct
     1621 ggacgaggac gccatgtccc gccgccccga cctgctggac gagctcgacc tcagcgacct
     1681 gaccgacacc ggctgggagg tgacgggccc ggccgaggag gccgacgggt ccacctggat
     1741 ccgggcgagc cacgacttcg ggacccccga ggagctcggg acgctggtcg acgacgtggc
     1801 cggggagcag ggcccgttcc gcgacttccg cctgacccgg gaggatgcct tcgccgagcg
     1861 ccgctacgac ttcaccggca ccgtcgactt cacggccggc gccggtgcgc tcgccgagga
     1921 cccggagctg gccgaggccc tggaggccga gccgatcgag ctgctcgagg agcgcctcgg
     1981 cgcggcgatc gacgagatgg tcgaggtgca ggtcgcggtg cgcatgcccg ggacggtgga
     2041 ctccaacgcc ctcacccagg cctccaacgg cgcggtctgg cgcccgtcgg tcgtggggcg
     2101 cgaggtcgtc gagctgcagg cgacggggac ggtgagccgc tccgaccggc tggtgtgggc
     2161 cggcgtcgcc gcagtcgcca ccttcgccct ggtgctctac ctgctgatcc gtgccgccgc
     2221 gtggcgccgg tcccggcgac ggtcgacccc cgccggctcc tagccatccc cgttcctgtc
     2281 ggagggtccg gagaggatgg ggcggtgacc acgacccggc cccgatcctc ccggccccgc
     2341 cgctccacgt tccggtgctc ctcctgcggg gcgggcgccc cggtgtgggc cgggcgctgc
     2401 accggctgcg acgagtggaa caccctcgtc gaggagcgca cgtccgccgt cacccgcacg
     2461 gtcggggcgc ccgccacgcc ggcccgaccc atcgccgaca tcaccgatca ggacgtcgcg
     2521 cccatgccca cgggcgtgcc cgagctcgat cgggtcctcg gcgggggact ggtccccggg
     2581 tcggtcaccc tcgtcggtgg cgagcccggc atcggcaagt cgaccctgct gctgcagacc
     2641 gcctcggcga tggccagcgg cggcaggtcc gcgctgtacc tgacggcgga ggagtccgca
     2701 ccgcaggtgc ggggacgggc ccagcgcctc ggcgccctcg ccgaccagct gtggctggcc
     2761 gccgacaccg ccctgcccga cgtgctcggc catctcgacg ccgtccagcc gtcggtgctc
     2821 atcgtcgact ccatccagac cgtccacgac cccgagctgg cgtcggcccc gggggcggtc
     2881 ggacaggtgc gcgagtgcgc ccagcgcctc gtcaccgagg cacgtgcgcg cggcgtggcc
     2941 gtggtgctgg tcgggcacgt caccaaggac ggcggcctcg ccggcccgcg ggtgctggag
     3001 cacctggtcg acaccgtcct gtccttcgag ggcgaccgcc accacgccct gcgcctgctc
     3061 cgggcggtga agcaccgctt cggcggcacg ggcgagctcg gcgtgttcga gatgtccgac
     3121 gccggcctcg tcggggtcga cgaccccagc gccctgttcc tggccgaccg tcgcacgggt
     3181 gaggccggct cggtcgtgat cccggtgctg gacgggggcc gtcccctcct cgtcgaggtc
     3241 caggccctgc tcgcgcccac caccatcccc acgccgcgcc gccagggaca gggcgtcgag
     3301 agcagccgtc tgggcatgct gctggcggtg ctcgaccagc gggtgggcgt gtcggccgcg
     3361 aacctcgacg tgtgggtcac cgtcgccggt ggggtccggg ccaccgagcc cgccgccgac
     3421 ctgggcgtcg cgctggccgt cgtgtcggcc atgaccggcg tgcccgtgcc cggcgacctg
     3481 gtcgcgtgcg gcgaggtcgg cctcgccggc gagctccgcc aggtgggccg catcgaccgc
     3541 cgcctcgccg aggccgcccg cctcggcttc cgtcgcgtcc tggtgcccac caccgccccc
     3601 gagccgccgg cgggctgcca ggcgatccgg gcgcgaggcc tgcgcgaagc catcgcagcc
     3661 gtcggcctgg accgctccgg atagtctgtg aaggtgccgt ccaggacctc acccgcgatg
     3721 ctggaggccc tggccttcgt cgcaccgggc gcgccgctgc gcgagggcct cgaccgcatc
     3781 ctgcaggccg agatgggcgc gctcatcgtc gtgggcgacg ggcccgacgt cttgaacatc
     3841 tgctcgggcg gcttcctgct cgacgccgcc ttcagccccc agcgcctctc cgagctggcc
     3901 aagatggacg gcgccatcat cctggcgccc gacgccaccc gcatcgcccg ggccaacgtc
     3961 cacctggtgc cgaacccgaa cgtgcccacg tcggagaccg gcacccggca ccg
//
LOCUS       DATISU010000372         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_634981, whole genome shotgun sequence.
ACCESSION   DATISU010000372 DATISU010000000
VERSION     DATISU010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634981"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1122)
                     /locus_tag="VLR27_14710"
     CDS             complement(67..1122)
                     /locus_tag="VLR27_14710"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSP04761.1"
                     /translation="MARIAVTGAAGPVGRRLCALLAEDPDVELVRAIDRRRVHLPDSS
                     RGDPRRERLDVRREHLREADLDDLLDGVDVVCHLAGSDPLEDTTADHDLLATSRLLEA
                     VERSGVRQLVARTSATVYGAWPDNPVPLTEAADLRPNPGTSWVQMRERIEVLLAAFAS
                     SHPDVVVAVLRPSVTVSDEGPDELGRVLAAARVVAPADGASPAQFVHVDDVVAAVDVV
                     RRNRAEGAFNVAPDGAIPADRIRALVGGAPRLPLPAPLARRLTAIGWRYRLAPTPPGF
                     VPFVTDSWVVANDRLRALGWEPKMTNDEAYVAGHAAAPWAQISPKRRQELALAAAAAV
                     VVAGGVGAAAAVRRTLR"
     gene            1141..>2074
                     /locus_tag="VLR27_14715"
     CDS             1141..>2074
                     /locus_tag="VLR27_14715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HSP04762.1"
                     /translation="MSGFGPTGDDNPFEGMPFLGDLGRMLGGMGGAAGGLQWDAARQM
                     AMQVASGGESEPNIDPIARMDVEQLARVADLHVAQKTGLSTSMTGRGVQVVPVTRMQW
                     VQRSLDAYKPLFESLASSLSTTPEAPSDPAEAADPFAAMLTQIMGSMAPMMLGVTAGG
                     MIGHLAQRSFGQYDLPIPREPSDELLLVLPNVNGFADDWSIDRKDLLLWLSMHEIAHH
                     AVLAVPHVRAALEGGLREYAAGFRPDASGLSDKLSTVDMSDPQASMTELQGLFGDPEV
                     VLGAIQTDAQRALLPRLEALIAMIEGYVDHVMDAI"
BASE COUNT          276 a          781 c          764 g          253 t
ORIGIN      
        1 gtgctcggca tccaccgggc cggcgagacc cgcgacgtgc gggtcaccct ggccggcaag
       61 ggctgatcag cgcagcgtcc gccgcaccgc cgccgcggcc ccgacgccgc cggccaccac
      121 cacggcagcg gcagcggcga gcgccagctc ctggcggcgc ttcggggaga tctgcgccca
      181 cggcgcggcc gcgtgaccgg cgacgtaggc ctcgtcgttg gtcatcttgg gctcccagcc
      241 cagggcccgg agccggtcgt tggccaccac ccacgagtcg gtgacgaagg ggacgaaccc
      301 gggcggggtc ggggccagcc ggtaccgcca gccgatggcg gtgaggcgcc gggccagggg
      361 cgcaggcagc gggaggcggg gcgccccgcc gacgagggcg cggatgcggt cggcggggat
      421 ggcgccgtcc ggcgccacgt tgaacgcccc ctccgcccgg ttgcgccgca cgacgtcgac
      481 cgcggccacg acgtcgtcga cgtgcacgaa ctgggccggc gaggcgccgt ccgccggggc
      541 gacgacccgc gcggcggcca ggacccggcc cagctcgtcg ggaccctcgt ccgacacggt
      601 cacgctcggg cgcaggacgg cgacgacgac gtcggggtgg gacgacgcga aggccgccag
      661 cagcacctcg atgcgctcgc gcatctgcac ccaggacgtc ccggggttgg ggcgcaggtc
      721 ggcggcctcg gtgagcggga ccgggttgtc cggccacgcg ccgtagacgg tcgccgacgt
      781 gcgggcgacg agctggcgga ccccggaccg ctcgaccgcc tcgaggagcc gggaggtggc
      841 gagcaggtcg tgatccgccg tcgtgtcctc gagcggatcg gagccggcca ggtggcagac
      901 gacgtcgacg ccgtcgagca ggtcgtccag gtcggcctcg cgcaggtgct cccggcggac
      961 gtcgaggcgc tcgcggcggg ggtcgccccg gctgctgtcg ggcaggtgga cccggcggcg
     1021 gtcgatggcc cggaccagct cgacgtcggg atcctcggcg agcagggcgc agagccgccg
     1081 gcccaccggg cctgctgcgc cggtgaccgc gatccgtgcc atcgccctac catggccgtg
     1141 atgagcggct tcggtccaac cggtgacgac aaccccttcg agggcatgcc gttcctcggc
     1201 gacctcgggc ggatgctcgg gggcatgggc ggcgcggccg ggggcctgca gtgggacgcg
     1261 gcccgccaga tggccatgca ggtggcatcg ggcggcgagt ccgagcccaa catcgacccc
     1321 atcgcccgca tggacgtcga acagctggcc cgggtggccg acctccacgt ggcccagaag
     1381 accgggctgt ccacgtcgat gaccggccgg ggcgtgcagg tcgtgcccgt cacccgcatg
     1441 cagtgggtgc agcgcagcct cgacgcctac aagccgctgt tcgagtccct cgcctcgtcg
     1501 ctgtccacca cgccggaggc accgtcggac ccggccgagg ccgccgaccc cttcgccgcc
     1561 atgctcaccc agatcatggg gtcgatggcc ccgatgatgc tgggcgtcac cgccggcggg
     1621 atgatcggcc acctggccca gcgcagcttc ggccagtacg acctgccgat cccccgcgaa
     1681 ccctccgacg agctcctgct ggtgctgccc aacgtcaacg gcttcgccga cgactggtcg
     1741 atcgatcgca aggacctcct gctctggctg tccatgcacg agatcgcgca ccacgccgtg
     1801 ctcgccgtgc cccacgtccg ggccgcgctc gaggggggcc tgcgcgagta cgccgccggg
     1861 ttccgccctg atgccagcgg cctctccgac aagctcagca ccgtcgacat gtccgatccg
     1921 caggcgtcga tgaccgagct ccagggcctg ttcggcgatc ccgaggtcgt gctcggcgcc
     1981 atccagaccg acgcccagcg cgccctgctc ccccgcctcg aggccctcat cgccatgatc
     2041 gagggctacg tcgaccacgt catggacgcc atcg
//
LOCUS       DATISU010000373         3779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_170151, whole genome shotgun sequence.
ACCESSION   DATISU010000373 DATISU010000000
VERSION     DATISU010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170151"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1520)
                     /locus_tag="VLR27_14720"
     CDS             complement(201..1520)
                     /locus_tag="VLR27_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HSP04763.1"
                     /translation="MVADPGTTGRAGKHRHLLAGSAALVVGAAVQAVTGAVFWLVAAR
                     MDTTDDVGRATALFTSILFIAYLAGLGLQVSLARFAPGRDRESHTVFSWAVLATVLSA
                     TVVAGGYLVVVDTEATRSLTDWHPVLGPVLFVSGAIGAALTLIVDVRWMTVRRWALVL
                     VRITVVGALRFPLLFLVPAGDHHTVHLFAVAALPLIVTGVGGVVAMPRFSGMGHRLFP
                     LPTSGGAAIRYSAVNYVSTLAYQAPQFVLPVIVLLSVDPDRNAAFYVAWGITSIVLYV
                     PMAIGQALLAEGGKDGAELRGQVRVVLVGTGALMGLAALVAWLGRELVTVVYGADYDD
                     AARILPALVVAGIPWAITSVYLTEARVLHRHLPTVAITGVLTVAILVPALVLVPDDGI
                     DGATRAFLVGNVAAAVVAIVFHRIGRRRRVTAVPEPGSVEDGLALHP"
     gene            1557..2489
                     /locus_tag="VLR27_14725"
     CDS             1557..2489
                     /locus_tag="VLR27_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP04764.1"
                     /translation="MTSPAETRTHPGARRPDRHRRVDTTGISLAVHEWGDEAAPPVLL
                     AHGGFDFAGTYDVFAPLLADAGWRVVSWDQRGHGDSEHAPLYQWEADIRDALAVFDSV
                     TTDPVPVVGHSKGGSLMSQLAASLPGRISSLVNIDGVPSRRSMPDVQDHDRTKLLADD
                     LAGWLDHRRRSATNSRKPGTIEELAQRRSQMNPRLSREWLEYLVTVGARRDEDGWRWK
                     IDPSLRMGGFGPWRPEWSLHRLPDITIPFLVLLGSEPEPMGWGTKTGDVWPFLPRQAR
                     AVEVPDVGHFVHIEAPEATAELVLEHLGAPGGRS"
     gene            2486..3151
                     /locus_tag="VLR27_14730"
     CDS             2486..3151
                     /locus_tag="VLR27_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP04765.1"
                     /translation="MKRTDLLHNHLTLALWELRGGAGRPLLLLHGLGERTTPDALDAV
                     ESWPGPVHGLDLTGHGGSDVPKGGGYYCEILMADADAALAALQPDADGPGVTVVGRGL
                     GGYVALLIAGARPDIVRGAVIADGPGLHGGGPGPTSPVLFRPDQDGSTPDPVALMELA
                     IDVRPPDYARTFAWQASALSGLDTAIAITAVNRPPWLAEVASQPGVVEAELPAALELF
                     GRT"
     gene            3203..3514
                     /locus_tag="VLR27_14735"
     CDS             3203..3514
                     /locus_tag="VLR27_14735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HSP04766.1"
                     /translation="MDRYQYLLIMAACAAITLPLEFVFRARVYRRPRRLLRAMWLPLA
                     IFIVWDIIAIARGHWTFDERWVTGWRLPGDIPVEEVAFFIVIPLCALLTFEASRHTLK
                     R"
     gene            3522..>3779
                     /locus_tag="VLR27_14740"
     CDS             3522..>3779
                     /locus_tag="VLR27_14740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HSP04767.1"
                     /translation="MSYEYAIPAVLSAVAVVALEKRVLRTGLFRSPSYWWTMGISLFF
                     MFLVNGWLTKLSAPIVRYDPDQKTPWRFPWDIPVEDYFFGFS"
BASE COUNT          548 a         1414 c         1335 g          482 t
ORIGIN      
        1 gccctgctcc tcggagcagc tgcctgcgga gacgacgacg acgacgtgaa caccgacgac
       61 atcgagcagg acgccgagga tctcggcgag agcaccggcg acgaggccga ggacctcggc
      121 ggcgagctcg aggaagaggg cgacgagatg gacgagtgag gctgaccgcc tcctcgtagg
      181 acccacatcg ctccaccccg tcaggggtgc agcgccaacc cgtcctcgac cgaccccggt
      241 tccggcacgg cggtgacccg tcgccgccgg ccgatccggt ggaacacgat ggcgacgacg
      301 gcggcggcca cgttcccgac caggaacgcg cgggtcgccc catcgatgcc gtcgtcgggc
      361 accagcacga gcgccggcac gaggatcgcc accgtcagca cgcccgtgat cgccacggtc
      421 gggaggtgac ggtggagcac gcgcgcctcg gtgaggtaca cggaggtgat cgcccacggg
      481 atgccggcca cgaccagcgc cggcaggatc cgggcggcgt cgtcgtagtc ggcgccgtag
      541 acgacggtga ccagctcccg gcccagccag gcgacgaggg cggcgaggcc catcagcgcg
      601 ccggtgccga cgagcaccac ccgcacctgg ccgcgcagct cggcgccgtc cttgccgccc
      661 tcggcgagca gcgcctgccc gatggccatc ggcacgtaga gcacgatgct ggtgatgccc
      721 cacgccacgt agaacgcggc gttgcggtcg gggtccaccg acagcagcac gatgacgggc
      781 aggacgaact gcggcgcctg gtaggcgagc gtggacacgt agttgaccgc cgagtaccgg
      841 atggcggcgc cacccgaggt gggcaggggg aacaggcggt gccccatgcc gctgaagcgg
      901 ggcatggcca cgaccccacc gaccccggtc acgatcaacg gtagggcggc cacggcgaac
      961 aggtgcacgg tgtggtggtc gccggccggc accaggaaca gcagcgggaa ccggagcgca
     1021 ccgaccacgg tgatccgcac gaggaccagc gcccaacggc gcaccgtcat ccaccgcacg
     1081 tcgacgatga gcgtgagcgc cgcgccgatg gcacccgaca cgaacagcac cggcccgagc
     1141 accgggtgcc agtcggtcag cgaccgcgtg gcctcggtgt cgacgacgac gaggtacccg
     1201 ccggcgacga ccgtggccga caggaccgtg gccagcacgg cccaggagaa gacggtgtgg
     1261 gactcccggt cgcggccggg cgcgaaccgg gcgagggaga cctgcagccc cagcccggcc
     1321 aggtaggcga tgaagaggat cgaggtgaag agcgcggtgg cccggccgac gtcgtcggtg
     1381 gtgtccatgc gggcggccac gagccagaag accgcaccgg tcaccgcctg tacggccgcg
     1441 cccacgacga gggcggccga cccggcgagc aggtggcgat gcttcccggc ccggccggtc
     1501 gtgccagggt cggcgaccat cgccggcgag gctacttcga cctatggtcc tggcctgtga
     1561 cgagccccgc cgagacccgc acccacccgg gtgcccgccg ccccgatcgc caccgccggg
     1621 tcgacaccac gggcatcagc ctggccgtgc acgagtgggg tgacgaggcc gcaccgcccg
     1681 tgctgctggc gcacggcggc ttcgacttcg ccggcaccta cgacgtgttc gcgccgctcc
     1741 tggccgatgc cggatggcgg gtcgtgtcct gggaccagcg cggccacggc gactccgagc
     1801 acgccccgct gtaccagtgg gaagccgaca tccgcgacgc cctcgccgtg ttcgactcgg
     1861 tgacgaccga cccggtgccc gtcgtcgggc actccaaggg cggctcgctg atgagccagc
     1921 tggccgcgtc gctgcccggc cgcatcagct cgctggtgaa catcgacggg gtgccgagcc
     1981 gccgctcgat gcccgacgtg caggaccacg accgcaccaa gctgctggcc gacgacctcg
     2041 ccggctggct cgatcaccgt cgccgctcgg cgaccaactc ccgcaagccg ggcaccatcg
     2101 aggagctggc ccagcggcgg tcgcagatga acccccggct gtcccgggag tggctggagt
     2161 acctcgtcac cgtcggtgcc cgccgggacg aggacggctg gcgctggaag atcgacccca
     2221 gcctgcgcat gggcggcttc gggccgtggc gaccggagtg gtcgctgcac cgactccccg
     2281 acatcaccat cccgttcctg gtgctgctcg gcagcgagcc cgagccgatg ggctggggca
     2341 ccaagacggg cgacgtgtgg cccttcctgc cccgccaggc tcgtgcggtc gaggtgcccg
     2401 acgtcggcca cttcgtccac atcgaggccc ccgaggccac ggccgagctg gtgctcgagc
     2461 acctgggcgc gccgggaggt cggtcgtgaa gcgcaccgac ctgctgcaca accacctcac
     2521 cctcgccctg tgggagctgc ggggtggcgc cggccgcccg ctgctgctcc tgcacggcct
     2581 cggcgagcgc accacccccg acgcgctcga cgccgtcgag tcctggcccg gcccggtcca
     2641 cgggctcgac ctcaccggcc acggcggatc cgatgtgccc aagggcggcg gctactactg
     2701 cgagatcctc atggccgacg ccgacgcggc cctcgccgcg ctgcagccgg acgcggacgg
     2761 ccccggcgtc acggtcgtgg ggcgcggcct cggtggctac gtggccctgc tgatcgccgg
     2821 cgcccgcccc gacatcgtgc ggggggccgt gatcgccgac ggtcccgggc tgcacggcgg
     2881 cggtcccgga ccgacgtcgc ccgtgctgtt ccggcccgac caggacggct ccaccccgga
     2941 ccccgtcgcc ctgatggagc tggccatcga cgtgcgcccg cccgactacg cccggacctt
     3001 cgcgtggcag gcgagcgcgc tctccggcct cgacaccgcc atcgccatca ccgccgtcaa
     3061 ccggcccccg tggctggccg aggtcgcctc ccaacccggt gtggtcgagg ctgagctgcc
     3121 cgccgcgctc gagctcttcg gccggacctg acccgggccg acgagggccg gaggggtcct
     3181 gcgggtaccg tcgcgcccgc ccatggaccg ctaccagtac ctgctcatca tggccgcctg
     3241 cgccgcgatc acgctgccgc tggagttcgt gttccgggca cgcgtgtacc gccggcctcg
     3301 ccggttgctg cgggccatgt ggctcccgct cgccatcttc atcgtgtggg acatcatcgc
     3361 catcgcccgg ggccactgga ccttcgacga gcggtgggtc accggctggc gcctgcccgg
     3421 cgacatcccg gtcgaggagg tcgccttctt catcgtgatc ccgctctgcg cgctgttgac
     3481 gttcgaagcc tcccggcaca cgctgaagcg gtgagcggcc catgagctac gagtacgcca
     3541 tccccgccgt gctgtcggcc gtcgccgtcg tcgccctgga gaagcgcgtg ctgcgcacgg
     3601 gcctgttccg cagcccgtcg tactggtgga cgatgggtat cagcctgttc ttcatgttcc
     3661 tggtcaacgg gtggctcacg aagctgtcgg cgccgatcgt gcgctacgac ccggaccaga
     3721 agaccccgtg gcgcttcccc tgggacatcc cggtcgagga ctacttcttc ggcttctcg
//
LOCUS       DATISU010000374         3545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_310067, whole genome shotgun sequence.
ACCESSION   DATISU010000374 DATISU010000000
VERSION     DATISU010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310067"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..235
                     /locus_tag="VLR27_14745"
     tRNA            161..235
                     /locus_tag="VLR27_14745"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:196..198,aa:Pro,seq:ggg)
     gene            354..1784
                     /locus_tag="VLR27_14750"
     CDS             354..1784
                     /locus_tag="VLR27_14750"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HSP04768.1"
                     /translation="MVFNSLVYALFLPAVVGLHWAVRPGARRWVLLVASYVFYAAWDW
                     RFLGLIFLSTAVDYTIGRALGVERTERARKALLITSVVVNLGILGVFKYGGFFVESAA
                     SLLARVGLEPNPALLQIVLPVGISFYTFQTISYTFDVYRRQLEPERNLSTFALYVAYF
                     PQLVAGPIERATHLLPQLRSERARPDAETIHSALGLILLGLVKKVAIADAVAPIVNRG
                     FGDAGNLSSLSAWTTMVAFALQVYGDFSGYTDVARGTSRLLGVELRVNFRQPYLSRNL
                     TEYWNRWHVSLSSWLRAYLYVPLGGNRHGRWTTYRNLMITMTLGGLWHGAAWTFVAWG
                     VLHGLILSIERALGVQPDDSDRLPALRHIPAILFAFLVWNVTLVPFRASSLGNAGEML
                     TSMFSGPLMPSSLGAWGDVLMVGVALVVVLAIDLVHRARPARLVETIRRPLEAPVASG
                     ALAGSAVVALVMFSGSPSDPFIYFQF"
     gene            1784..2737
                     /locus_tag="VLR27_14755"
     CDS             1784..2737
                     /locus_tag="VLR27_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04769.1"
                     /translation="MLARSTKMLASLVAVLLVVEAAVRVVSSGFPEPVTWYHEIAQAK
                     VEQLIDLDDDLDHVFIGTSQTYHGIDPAVVDRRLGTRSYNAAIPAGIPPIQHRWLEDA
                     VLRHRDVDTVVWPMSSIDLNAARPQDMRPIYDDAFEVRGGVLAGVDRWLSARLAMFRH
                     RRVLADSRSWFDADDPVEHARGVLQANGKRRPGRANTSDAERRRVVRDVIGDYEVGGH
                     MSDDIHRTVASLRERNVDVVFVWLPQAPRFLDLLPDRSADADAEREARRLAAELDVSF
                     IDVSDGYGNGDFSDFTHLTGEAAQRLSATVADELAELGGGH"
     gene            complement(2900..3094)
                     /locus_tag="VLR27_14760"
     CDS             complement(2900..3094)
                     /locus_tag="VLR27_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04770.1"
                     /translation="MEERRIDETEVRSERRSGRGPIIGLLVVLGLVLVVLILFLLSGD
                     GDDSGDDINPADIEVDIGDE"
     gene            complement(3218..>3545)
                     /locus_tag="VLR27_14765"
     CDS             complement(3218..>3545)
                     /locus_tag="VLR27_14765"
                     /inference="COORDINATES: protein motif:HMM:NF019191.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HSP04771.1"
                     /translation="STADATYGVDAQNADWNAQAAMSAADYLDYTAFSRPGLISQLEY
                     EGFSTADATYGVDAQNADWNAQAAMSAADYLEYTSFSRSGLIDQLIYEGYTPEQAEYG
                     VSTTGL"
BASE COUNT          469 a         1275 c         1166 g          635 t
ORIGIN      
        1 cccgagccgc tcccgccggt cccgtggcac ttcaagctga tgctggcgct gctggtcgcc
       61 tacctgctct ggcgcttcgt gcagttcctc gccgccgtgt tctgagcatc gggcggattg
      121 gcggatcgcc accagcaggg gagtagattc gaccgtccca cgggctgtgg cgcagcttgg
      181 ttagcgcgct tgactggggg tcaagaggtc gggagttcga atctccccag cccgacggtg
      241 cgaagggtcg ctgactgcgg agacgcaggc ggcggccctt cgtcgttctg gcaggactca
      301 cgacgacagg aacggggtag gaccccccgg gtcccgaccc gaagtcgctc cccatggtct
      361 tcaactcgct cgtctacgcg ctgttcctgc ccgcagtggt cgggctccac tgggctgtcc
      421 gccccggcgc tcggcggtgg gtcctcctgg tggcgtcgta cgtcttctac gccgcctggg
      481 attggcgctt cctcggcctg atcttcctgt cgaccgccgt cgactacacg atcggccgag
      541 cgctgggagt cgagcgcacc gagcgcgctc gcaaggcact gctgatcacc agcgtggtgg
      601 tcaacctggg gatcctcggg gtgttcaagt acggcggctt cttcgtcgag tcggccgcat
      661 cgctgctcgc tcgggtcggt ctggaaccca acccggcgct gctccagatc gtcctcccgg
      721 tcggcatcag cttctacacg ttccagacga tctcgtacac cttcgacgtc taccgccggc
      781 agctcgaacc cgagcgcaac ctgtcgacgt tcgccctgta cgtggcgtac ttcccccagc
      841 tcgtcgcggg tccgatcgag cgggccaccc acctgctgcc ccagctgcgc tccgagcgcg
      901 cccggcccga tgccgagacg atccactccg cgctcgggct gatcctgctc ggcctcgtaa
      961 agaaggtggc gatcgccgat gcggtggcgc cgatcgtgaa ccggggcttc ggcgacgccg
     1021 ggaacctctc cagcctgtct gcctggacga cgatggtcgc cttcgctctg caggtgtacg
     1081 gcgacttctc cggctacacc gacgtcgccc gcggcacctc gcggctgctg ggcgtggagc
     1141 tgcgggtcaa cttccgccag ccgtacctgt cacgcaacct caccgagtac tggaaccgat
     1201 ggcacgtctc gctgtcgtcg tggctgcggg cgtacctcta cgtcccgctc ggcggtaacc
     1261 gccacggacg ctggacgacg taccggaacc tgatgatcac catgaccctc ggcgggctct
     1321 ggcacggtgc ggcgtggacg ttcgtggcgt ggggcgtgct gcacggcctg atcctctcca
     1381 tcgaacgggc cctgggcgtg cagcccgacg acagcgatcg gctccccgcc ctccggcaca
     1441 tccccgccat cctcttcgca ttcctggtct ggaacgtgac cctggtgccg ttccgggcca
     1501 gctcgctcgg caacgccggc gagatgctga cctcgatgtt cagcggaccc ctgatgccgt
     1561 cgtcgctcgg ggcctggggt gacgtcctga tggtcggcgt ggcgctcgtc gtcgtgctgg
     1621 ccatcgacct ggtccaccgt gcccggccgg cgcgcctcgt ggagacgatc cgccgaccgc
     1681 tcgaggcccc cgtcgcctcc ggtgcgctcg ccggctcagc cgtggtggcg ctcgtgatgt
     1741 tcagcggctc cccgtccgac cccttcatct acttccagtt ctgatgctcg ctcgatccac
     1801 caagatgctg gccagcctcg tggcggtcct cctcgttgtc gaagccgcgg tgcgcgtcgt
     1861 ctcgtcgggg ttcccggagc ccgtgacctg gtaccacgag atcgcccagg ccaaggtcga
     1921 gcagctcatc gacctggacg acgacctcga ccacgtgttc atcgggacct cgcagaccta
     1981 ccacggcatc gatccagcgg tggtcgaccg acggctcggc acccgcagct acaacgccgc
     2041 catcccagcc ggcatcccgc cgatccagca ccgctggctg gaggacgcag tcctgcgcca
     2101 ccgcgatgtc gacacggtcg tgtggcccat gtcgtcgatc gacctgaacg ccgcgcgccc
     2161 ccaggacatg cgaccgatct acgacgacgc gttcgaggtg cgcggcgggg tgctcgctgg
     2221 tgtcgaccgg tggctctcgg cgcgcctggc catgttccgc catcgtcggg tcctggcgga
     2281 ctcccgctcc tggttcgacg cggacgaccc ggtggaacac gctcgggggg tcctgcaggc
     2341 caacggcaag cgccggcccg gacgtgccaa caccagcgac gccgaacgcc gtcgcgtcgt
     2401 gcgcgacgtg atcggcgact acgaggtcgg cggacacatg tccgatgaca tccaccgcac
     2461 ggtcgcctcc ctgcgcgagc ggaacgtcga cgtggtcttc gtgtggctgc cgcaggcacc
     2521 ccgcttcctc gacctgctcc cggaccggtc agccgacgcc gacgcggagc gagaggctcg
     2581 acggctcgct gccgagctgg acgtctcctt catcgatgtc agcgacgggt acgggaacgg
     2641 ggacttcagc gacttcaccc acctgaccgg cgaggccgcg cagcggctgt cggccacggt
     2701 ggccgacgag ctcgctgagc tcgggggcgg gcactagctc gacggtcgag gatctccggt
     2761 ggggctcggc gcgccctacg cttcccagca acgggccagg ttctcgccgg acctgcgctc
     2821 gccagagggc ctcatctccc acaggggatg gggccctcgt cgcgtctgga ccaccccggg
     2881 gcgttcgctc gccccgcact cactcgtcgc cgatgtcgac ctcgatgtcc gctggattga
     2941 tgtcgtcgcc gctgtcatcg ccgtcaccgc tcagcaggaa caggatcagg accacgagga
     3001 ccaggcccag gacgacgagc agtccgatga tcggccctcg tccgctgcgc cgttcgctcc
     3061 ggacctctgt ctcgtcgatc cttcgctctt ccatgggacc ctcctggcac gggttggtga
     3121 ggtcgcgaac cgtacctggg gctgtggcgt tgcgctgacg caacgtcgtg ctcccggttc
     3181 gccagctgcc gacgtgggct gtgcactcgc ctgcgatcta caaccccgtg gtgctgacgc
     3241 cgtactcggc ctgctccggc gtgtagccct cgtagatgag ctggtcgatc aggccactgc
     3301 gggagaacga ggtgtactcc aggtagtccg ccgccgacat cgccgcctgc gcgttccagt
     3361 cggcgttctg ggcgtcgacg ccgtacgtgg cgtcggcggt gctgaagccc tcgtactcga
     3421 gctggctgat gagtccgggg cgggagaacg ctgtgtagtc caggtagtcc gccgccgaca
     3481 tcgccgcctg cgcgttccag tcggcgttct gggcgtcgac gccgtacgtg gcgtcggcgg
     3541 tgctg
//
LOCUS       DATISU010000375        10354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_402986, whole genome shotgun sequence.
ACCESSION   DATISU010000375 DATISU010000000
VERSION     DATISU010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 10354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10354
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402986"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1237)
                     /locus_tag="VLR27_14770"
     CDS             complement(92..1237)
                     /locus_tag="VLR27_14770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP04772.1"
                     /translation="MEKVLDRATQVAHEVAAPAAETVDAEARWPEAALRALQDAGLGG
                     LVVPERDGGLGHGLTALAQVCEILGGACASTAMCFGMHGVGSAVIAAKATEEQQDRYL
                     EPIVAGRHLTTLTLSEPGTGSHFWLPQSQLRRADDGFVLSGTKTFVTSGSHADSYVVS
                     AVDADATSPIGEFSCVVVDAANPGLEWGPPWDGLGMRGNSSRSVELRDVRIPEAGLLG
                     EPGDQIWYVFNVIAPYFLMAMSGSYLGVAAGAFEDMRLHVTQRRYSHDGSRLAEQPVI
                     QLRVGELWAELERTRQLVRHAAAGADRGEPWALPALASAKAEVAECATNVVNGAMTLV
                     GGIGYREHGSLGRRLRDARAAHVMAPTTDLLRLWTGRAVLDEHILAD"
     gene            complement(1344..2516)
                     /gene="argJ"
                     /locus_tag="VLR27_14775"
     CDS             complement(1344..2516)
                     /gene="argJ"
                     /locus_tag="VLR27_14775"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HSP04773.1"
                     /translation="MQLPAGFRAHTANIGIKDDTTDFAVIAAERPCSAAAVFTRSRFA
                     GPSVTLSRGHAGDGRLQAVVVVSKNANVATGADGDADALELVEGVAHALGCDPTDVLV
                     ASTGVIGRRYPMDRVRQHLASMPPPRSTDAGAVAEAIMTTDTHPKTAAATAPTVTGGE
                     ARVVGIAKGVGMIEPDMATMLAFVFTDADVDAAVLDRAFRAVVERTFNSLSVDTDTST
                     SDTAAVLASGAAGPVDEADLEAALDAVCLALTRQLAADGEGAETLVVVTVDEARDRDQ
                     AKRVAKTIVNSPLVKTAVHGADPNWGRIAMAVGKCSDDTDIDQRAVTIRIGDRELYPK
                     PASETDLDALVDHLRGDEVSIHVSLGVVRDGAGASATVYGCDLTDGYVRINADYTT"
     gene            complement(2544..4298)
                     /locus_tag="VLR27_14780"
     CDS             complement(2544..4298)
                     /locus_tag="VLR27_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04774.1"
                     /translation="MSDRPEDPGMPGGPPTEPPPPPPPPVQGATVTLDSETEVRAGGV
                     GGRIVAGAVGLVLLLGGVAFAATQIGSDDGASDPEAAVAELFDAIADEDVLGMLATLD
                     PGERDALSQPVERFFGELERLEVLDDSFELSGVAGVDLEFEDLTFRTEPVRSDLTRVY
                     LTGGTASFAVDTDEIPVGDFLADTFDRFGVEYRGVQQSESDTLDPAEADDTFLVVRDT
                     GDGWRVSLGYTAVEAARVAQGAPVPAAGAGLAAIGADTPEEAVEGFLQAAVGIDIEGV
                     VARLSPGELRAVHDYWPVLVGGTDLPTADDVPADIALTDLSLRSSTDGDRGRVFIDSI
                     GVDVVTEDFEGGATIADGCIEVRGDIRDELDAAEVELPDGPICRDDIDAILEDAAEEM
                     GGANVFGFGGMDGLAMGDGDAPELGITVVHIDEKGWFVAPMGTWADLGLAVLETIDRE
                     DLDAMVDAVEEFFGSGFFGGGMLGGGFVPPGMTDDLGGFEDFDDEFGYGETEVEVETF
                     EELGDPIGGNDLDATLAEMVEVFAGDPDVAACALEQLRLKATPDQLFELADAYQYEYE
                     PSMEAQDVLFGVLSSCGG"
     gene            complement(4295..4720)
                     /locus_tag="VLR27_14785"
     CDS             complement(4295..4720)
                     /locus_tag="VLR27_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04775.1"
                     /translation="MTTARRRDLAAGAAGVLALVTTATTFLGWGRSGQRVRTSYELVD
                     VADRAGVLADDAAWAAPLWFLAPAACGAVLVALAVHRHAIAGALTTTLGALVGTGAIL
                     VDRSPLVAEPAVGVAVVLGGATALSGVAVLVTARKETAG"
     gene            complement(4717..5379)
                     /locus_tag="VLR27_14790"
     CDS             complement(4717..5379)
                     /locus_tag="VLR27_14790"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSP04776.1"
                     /translation="MTAERDAFPRTERTTIGRSPDRGTRDRSVAEAILDEGMLCHVGL
                     ATDHGPLVIPTTYARRGDELLIHGSPAATWLRATGKGEPVCVTVTLLDGLVLARSTFH
                     HSMNYRSVVVFGRAEPITDHEEKAAALDAIVEHLVPGRTAEVRSMRDDEIRGTLVVRL
                     ALDEASTKIRTGGPLDDEADADLVDVWAGVLPMRTAFGEPEPDDLAAAVPPPPSVVDY
                     GR"
     gene            complement(5425..5736)
                     /locus_tag="VLR27_14795"
     CDS             complement(5425..5736)
                     /locus_tag="VLR27_14795"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HSP04777.1"
                     /translation="MSTAVVVIIGLGLGTYVLKSAGPLLLGGRRLPAPVERIAQQLPA
                     ALLAALVVVSTVAGDRALVLDARLAGVVAGGVALRLRAPFIVVVAVAVAVTGVVRAAA
                     G"
     gene            complement(5733..6419)
                     /locus_tag="VLR27_14800"
     CDS             complement(5733..6419)
                     /locus_tag="VLR27_14800"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HSP04778.1"
                     /translation="MDDRTRRRRIHRQALSISLALAPFGIVFGVACVEAGLGIAEAVG
                     FSAIVFTGSAQFAAVGVLGDGGGALAAISAGLLLNIRSLAFGVLLAPALTGRVGTRAL
                     LAQLVIDESTAVATVQDEPRWQRYGFLAGGLAVFTVWNLTTVAGVLLASVDDEFITDL
                     GFDAAAPAAFLALLWPRLVGDGGSEGRRIAIAGGLVAVAAVPLAPPGIPILLAGLGVL
                     AVQRRREVQA"
     gene            6479..7240
                     /locus_tag="VLR27_14805"
     CDS             6479..7240
                     /locus_tag="VLR27_14805"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:NF002198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HSP04779.1"
                     /translation="MSDLLLGSHVAPDDPLGAAAAEGAECVQIFLGDPQSWKKPKPRD
                     DADALKAADIPIYVHAPYLINVASPNNKVRIPSRKILQQTCDAATDIGAKAVIVHGGH
                     VTEDDDEADGFVRWRKALDSLETDMPVYLENTAGGDHAMARHFDTIGRLWEHIADTGI
                     GFCLDTCHAWAAGEELVEAVERIVGITGRVDLVHCNDSKDECGSGRDRHENLTKGQID
                     PEAILHVVRTAGAPVICETADVGRKDDLAWLRDNL"
     gene            7327..7554
                     /locus_tag="VLR27_14810"
     CDS             7327..7554
                     /locus_tag="VLR27_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04780.1"
                     /translation="MRRRHAILATTAGAAMLGGLLLAPQAQAEPTLCVDLDLTVQDQE
                     LVQSICLPPEDGGGAPALPGLPGEPALPALP"
     gene            complement(7623..9491)
                     /locus_tag="VLR27_14815"
     CDS             complement(7623..9491)
                     /locus_tag="VLR27_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP04781.1"
                     /translation="MRQGPHSMMPSDASAVDGASLDRSIVRRVMEFARPYKTQIIGFL
                     AAIITAAILGLAPPLLFKWILDDAIADGDKGRLSLLAGLAVAAAVADSGLSLVERWLS
                     SRIGEGLIFDLRVRVFDHVQRLPVAFFTRAQTGALVSRLNNDVIGAQRALTGTLGSVV
                     SNVVVLVTTVAAMVALEWRLTLFTIALLPAFLIPARRVGTRLQVISREQMERNARMNS
                     TMTERFGVAGAMLVKLFGRHDDESDRFADDAAGVRDAGVRSAVLSRVFFSAIALVGAL
                     GIAAVYWWGGILVIDGAITLGTLVALAALVQRIYQPLTALTNARIDIMTAFVSFDRVF
                     EVLDIRNPIEDRPGAQDLADVQGRVDFDDVWFTYPTAQDVSVPSLAGAVPDHDASSGP
                     VLRGIDAHVDPGQLVAIVGPSGAGKTTLTSLLPRLYDVDSGAVRIDGHDVRDLTQESL
                     RLAIGVVSQDPHLFHDTIAGNLRYARPDATIDELRQACEAARIHHVIAALPEGYDTVV
                     GDRGYRMSGGEKQRLSIARMLLKDPAIVILDEATSHLDSENEAAVQAALAEALVGRTS
                     LVIAHRLSTITTADQILVVDDGRVVESGRHEELLEAAGLYADLYRTLVRGEPAPSA"
     gene            complement(9599..9688)
                     /locus_tag="VLR27_14820"
     tRNA            complement(9599..9688)
                     /locus_tag="VLR27_14820"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9652..9654),aa:Ser,seq:cga)
     gene            complement(9736..10200)
                     /gene="tadA"
                     /locus_tag="VLR27_14825"
     CDS             complement(9736..10200)
                     /gene="tadA"
                     /locus_tag="VLR27_14825"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HSP04782.1"
                     /translation="MPEPAAPTDDELMCLALEEARLAADAGEVPVGAVVVVGGQVVAR
                     GRNRRQGDHDPSAHAELLALRAACADAGSWRLPGAQVVVTLEPCPMCAGALVAARVGR
                     VVFGAADPKAGALGSLYNLGVDPRLPHELDVRAGVRAEECGALLAGFFAERR"
BASE COUNT         1464 a         3639 c         3926 g         1325 t
ORIGIN      
        1 cgggatccgg ccggtgcggg caccgatctg gcgcacgatc gcgtcgggga ggtcgacgac
       61 cacgatgtcg gtcacggcgg gcgggtgagg ctcagtcggc gaggatgtgc tcgtcgagga
      121 cggcacgtcc ggtccagagg cgcagcaggt cggtggtggg tgccatgacg tgggcggccc
      181 gggcgtcgcg caggcgccgg ccgaggctgc cgtgctcccg gtaaccgatg ccgccgacca
      241 gggtcatggc cccgttgacc acgttggtgg cgcactcggc cacctcggcc ttcgcggacg
      301 ccagcgccgg caacgcccag ggttcgcccc ggtcggcccc ggcggccgcg tggcggacga
      361 gctggcgggt gcgctcgagc tcggcccaga gctcgccgac gcggagctgg atgacgggct
      421 gctcggccag gcggctgccg tcgtgggagt agcggcgctg ggtcacgtgg aggcgcatgt
      481 cctcgaacgc gccggcggcg acgccgaggt agctgcccga catggccatg aggaagtacg
      541 gggcgatgac gttgaagacg taccagatct ggtcgccggg ctcgccgagc agccccgcct
      601 cggggatccg gacgtcgcgc agctccacgg agcggctgct gttgccgcgc atgccgagcc
      661 cgtcccaggg cggtccccac tcgagcccgg ggttcgccgc gtcgacgacc acgcaggaga
      721 actcgccgat cggagacgtg gcgtcggcgt cgacggccga gacgacgtag gagtcggcgt
      781 gggatccgct ggtcacgaag gtcttggtgc cggacaggac gaagccgtcg tcggcccggc
      841 gcagctggga ctgggggagc cagaagtgcg agcccgtccc gggctcgctg agcgtcagcg
      901 tggtgaggtg gcggccggcg acgatgggct cgaggtagcg gtcctgctgc tcctcggtcg
      961 ccttggcggc gatgaccgcg gacccgaccc cgtgcatgcc gaagcacatc gccgtcgagg
     1021 cgcaggcgcc acccaggatc tcgcacacct gggcgagggc ggtcaggccg tggcccaggc
     1081 cgccgtcgcg ctcgggcacg acgaggccgc ccagcccggc gtcctgcagc gcacgcagcg
     1141 ccgcctccgg ccagcgggcc tcggcgtcga cggtctcggc cgcgggcgcg gcgacctcgt
     1201 gcgcgacctg ggtcgctcga tcgagcacct tctccatcga gagactcgtc aaggggttct
     1261 ccacccggtt cggcccgccg ccttcactgt gtgcgtccgc gaatgtacgc catgcgaggt
     1321 cgtggcgtgc acctcggcag atgtcaggtg gtgtagtccg cgttgatgcg cacgtagccg
     1381 tcggtgaggt cgcagccgta cacggtggcg gacgctccgg cgccgtctcg caccacgccg
     1441 agcgagacgt ggatgctgac ctcgtccccc cggaggtggt cgacgagcgc atcgaggtcg
     1501 gtctcgcttg ccggcttcgg gtacagctcc cgatctccga tccggatcgt cacggcccgc
     1561 tggtcgatgt cggtgtcgtc gctgcacttg ccgaccgcca tggcgatccg gccccagttc
     1621 gggtcggcgc cgtgcacggc ggtcttcacc aggggggagt tgacgatggt cttggccacc
     1681 cgcttggcct ggtcgcggtc ccgcgcctcg tcgacggtga cgaccacgag ggtctcggcg
     1741 ccctcgccgt cggcagcgag ctgccgggtg agggcgaggc agaccgcgtc gagggcggcc
     1801 tccaggtcgg cttcgtccac cggcccggcc gcgccgctgg ccagcacggc cgcggtgtcg
     1861 gaggtcgagg tgtcggtgtc gaccgacagc gagttgaacg tgcgctccac caccgcccgg
     1921 aaggcgcggt cgagcacggc ggcgtcgacg tcggcgtcgg tgaacacgaa cgccagcatc
     1981 gtggccatgt cgggctcgat catcccgacg cccttggcga tgcccaccac gcgggcctcg
     2041 ccgccggtca cggtgggcgc ggtggccgcc gcggtcttcg ggtgggtgtc ggtcgtcatg
     2101 atcgcctcgg ccacggcacc ggcgtcggtc gaccgaggag gtggcatcga ggcgaggtgc
     2161 tggcggaccc ggtccatcgg gtagcggcgt ccgatcaccc cggtggaggc cacgagcacg
     2221 tcggtggggt cgcacccgag ggcgtgggcc acgccttcga ccagctcgag cgcgtcggcg
     2281 tcgccgtcgg ccccggtggc cacgttggcg ttcttggaca ccacgaccac cgcctggagg
     2341 cggccgtcac ctgcgtgccc gcgcgacagg gtgacgctcg ggccggcgaa ccgggagcgg
     2401 gtgaacaccg cggccgccga gcaggggcgc tcggcggcga tgaccgcgaa gtcggtggtg
     2461 tcgtccttga tcccgatgtt ggcggtgtgg gcccggaagc cggcagggag ctgcacggcg
     2521 ccgagtctgg caccgcccga cggctacccc ccgcaggagg agaggacgcc gaagagcacg
     2581 tcctgggcct ccatcgaggg ctcgtactcg tactggtagg cgtcggcgag ctcgaagagc
     2641 tggtcggggg tcgccttcag acgcagctgc tcgagggcgc aggcggcaac gtcgggatcg
     2701 ccggcgaaga cctcgaccat ctcggccagc gtggcgtcga ggtcgttgcc gccgatcggg
     2761 tcgccgagct cctcgaaggt ctcgacctcg acctcggtct ccccgtaccc gaactcgtcg
     2821 tcgaagtcct cgaatcctcc gaggtcgtcg gtcatccccg gcgggacgaa cccgccgccg
     2881 agcatgccgc cgccgaagaa cccgctgccg aagaactcct cgacggcgtc gaccatcgcg
     2941 tcgaggtcct cgcggtcgat ggtctccagc acggcgaggc cgaggtcggc ccacgtcccc
     3001 atgggtgcca cgaaccagcc cttctcgtcg atgtggacga ccgtgatgcc gagctcgggg
     3061 gcgtccccgt cgcccatggc gaggccatcc atcccgccga acccgaacac gttcgcgccg
     3121 cccatctcct cggccgcatc ctcgaggatg gcatcgatgt cgtcccggca gatggggccg
     3181 tcgggcagct cgacctcggc cgcgtcgagc tcgtcgcgga tgtcgccgcg gacctcgatg
     3241 cacccgtcgg cgatggtggc cccaccctcg aagtcctcgg tgaccacgtc gacgccgatg
     3301 ctgtcgatga acacccgtcc ccggtcgccg tcggtggagg accgcagcga caggtcggtc
     3361 agcgcgatgt cggcggggac gtcgtccgcg gtggggaggt cggtaccacc gacgaggacc
     3421 ggccagtagt cgtgcaccgc ccgcagctcg ccgggggaga gtcgggcgac gacgccctcg
     3481 atgtcgatgc cgaccgcggc ctgcaggaag ccttcgacgg cctcctcggg cgtgtcggcg
     3541 ccgatggcgg cgagcccggc gccggcggcg ggcaccggcg cgccctgcgc gacccgggcg
     3601 gcctccacgg cggtgtagcc gaggctgacc cgccacccgt cgccggtgtc gcgcacgacg
     3661 aggaaggtgt cgtcggcctc ggcggggtcg agcgtgtcgg actcggactg ctggaccccc
     3721 cggtactcga cgccgaagcg gtcgaaggtg tcggcgagga agtcgcccac cgggatctcg
     3781 tcggtgtcga cggcgaagga tgcggtcccg ccggtcaggt agacgcgcgt caggtcgctg
     3841 cgcacgggct cggtgcggaa ggtcaggtcc tcgaactcga ggtcgacgcc ggcgacgccg
     3901 ctgagctcga aggagtcgtc gagcacctcg aggcgctcca gctcgccgaa gaaccgctcg
     3961 accggctggg agagcgcgtc gcgctcaccg gggtcgaggg tggcgagcat gccgaggacg
     4021 tcctcgtcgg cgatggcgtc gaacagctcg gcgacggcgg cctccgggtc gctcgccccg
     4081 tcgtcgctgc cgatctgggt ggcggcgaag gccacgcccc cgagcagcag gaccaggccg
     4141 accgcaccgg cgacgatgcg cccgccgacg ccaccggcgc gcacctcggt ctcggagtcg
     4201 agggtgaccg tcgcaccctg caccgggggc ggtggcgggg gcggcggctc cgtcggcggt
     4261 ccgcccggca tgccggggtc ctctggtcgg tcgctcatcc cgccgtctcc ttccgtgccg
     4321 tgacgagcac ggcgacacca ctcaaggccg tggccccacc gaggacgacg gccaccccga
     4381 ccgccggttc ggcgacgagt ggtgaccggt cgaccaggat cgcgcccgtg cccaccagcg
     4441 caccgagcgt agtcgtgagc gccccggcga tggcgtgtcg gtgcacggcg agggcgacca
     4501 ggaccgcacc gcaggccgcc ggtgcgagga accacagcgg tgccgcccac gcggcgtcgt
     4561 cggcgaggac gccggcccgg tcggcgacgt cgaccagctc gtagctggtg cggacgcgct
     4621 gcccggagcg cccccagccc aggaaggtgg tggcggtcgt gacgagggcg aggacgccgg
     4681 ccgcccctgc cgcgaggtct cgtcgccgcg ccgtcgtcat cggccgtagt cgaccacgct
     4741 cggtggtggg gggacggcgg cggcgaggtc gtcgggctcg ggttcgccga acgccgtccg
     4801 catcgggagc acgccggccc agacatcgac caggtcggcg tcggcctcgt cgtcgagggg
     4861 cccacccgtg cggatcttgg tggatgcctc gtcgagggcc agtcgcacga cgagcgtgcc
     4921 gcggatctcg tcgtcgcgca tcgagcggac ctccgcggtg cggcccggca cgaggtgctc
     4981 gacgatcgcg tcgagggcgg ccgccttctc ctcgtggtcg gtgatcggct cggcccgacc
     5041 gaacacgacg acggaccggt agttcatcga gtggtggaac gtgctgcggg ccaggacgag
     5101 cccgtcgagc agggtgacgg tgacgcagac cggttcgccc ttgccggtgg cccgcagcca
     5161 cgtggccgcc ggcgagccgt ggatcagcag ctcgtcgccc cgccgggcgt aggtggtggg
     5221 gatgacgagg gggccgtggt cggtggcgag cccgacgtgg cacagcatcc cctcgtcgag
     5281 gatcgcctca gcgaccgagc gatcgcgggt gccgcgatcc gggctgcgcc cgatggtggt
     5341 ccgctcggtg cgggggaagg cgtcgcgttc ggcggtcacg ggtccgcagc caaggcgatg
     5401 cggaccgacc ggcgcacatc ggtttcagcc ggccgcggcc cgcacgacac cggtgaccgc
     5461 cacggccacg gcgaccacga cgatgaaggg cgcccggagg cgcagggcca cgccgccggc
     5521 gaccaccccg gcgaggcgcg cgtcgagcac cagcgcccgg tcgcccgcca ccgtgctcac
     5581 gaccacgagc gcggcgagca gcgcagcagg cagctgctgc gcgatgcgct ccaccggggc
     5641 gggcagacga cgtccgccga ggagcagcgg gccggcggac ttgaggacgt aggtgcccag
     5701 accgaggccg atgatgacga cgaccgcggt gctcacgcct gcacctcccg ccggcgctgg
     5761 accgccagga cgccgaggcc ggcgagcagg atcgggatgc cgggcggtgc cagcggcacg
     5821 gcggcgacgg ccaccaggcc gccggcgatc gcgatgcgcc gaccctcgct cccgccgtcc
     5881 ccgacgagtc gcggccacaa cagcgcgagg aaggcggcgg gcgcggcggc gtcgaagccg
     5941 aggtcggtga tgaactcgtc gtcgacggat gccagcagca cgccggcgac cgtggtgagg
     6001 ttccagacgg tgaacacggc cagcccgccg gcgaggaagc cgtagcgctg ccagcggggt
     6061 tcgtcctgga cggtcgccac ggcggtcgac tcgtcgatca ccagctgggc caggagcgcg
     6121 cgggtcccga cccgaccggt cagggcgggg gcgaggagca ccccgaaggc cagggaccgg
     6181 atgttcagca gcaggccggc gctgatcgcc gccagcgcac cgccgccgtc gccgagcacg
     6241 ccgaccgcgg cgaactgggc cgagccggtg aacacgatcg ccgagaaccc gaccgcctcg
     6301 gcgatgccga gcccggcctc gacgcaggcc acgccgaaca cgatcccgaa gggcgcgagg
     6361 gccagggaga tggagagcgc ctggcggtgg atgcggcggc gccgggtgcg atcgtccacg
     6421 acggtgcatc gtgcccgtcg ggtcggcgcc ccgcccaacc cccactaccg tcggggccat
     6481 gtcggacctg ctgctcggaa gccacgtcgc ccccgatgat ccgctcggcg cggccgccgc
     6541 cgagggcgcc gagtgcgtcc agatcttcct cggcgacccg cagtcgtgga agaagcccaa
     6601 gccccgggac gacgccgacg cgctcaaggc cgccgacatc ccgatctacg tccacgcccc
     6661 gtacctgatc aacgtggcgt cgccgaacaa caaggtccgc atcccgagcc ggaagatcct
     6721 gcagcagacc tgcgatgccg ccaccgacat cggcgcgaag gcggtcatcg tgcacggcgg
     6781 tcacgtcacc gaggacgacg acgaggccga cggcttcgtg cgctggcgca aggcgctcga
     6841 cagcctcgag accgacatgc ccgtctacct ggagaacacc gccgggggcg accacgccat
     6901 ggcccgccac ttcgacacca tcggccggct gtgggagcac atcgccgaca ccggcatcgg
     6961 cttctgcctg gacacctgcc acgcgtgggc ggcgggggag gagctggtcg aggcggtcga
     7021 gcgcatcgtc ggcatcacgg gtcgggtcga cctcgtccac tgcaacgact ccaaggacga
     7081 gtgcgggtcc ggccgggacc gccacgagaa cctcaccaag ggccagatcg accccgaggc
     7141 gatcctccac gtcgtgcgca ccgcgggcgc cccggtgatc tgcgagaccg ccgacgtcgg
     7201 tcgcaaggac gacctggcct ggttgcgcga caacctctga gcgattttcg caaggttcgg
     7261 acgtcggctg cgttctcctc ggaccggctg gcacaggggt gccggccacg acgaaaggga
     7321 tcgacgatgc gtcgacgcca cgccatcctc gccacgaccg ccggtgccgc catgctgggt
     7381 gggctcctcc tcgcccccca ggcccaagcc gagcccacgc tctgcgtgga cctcgacctg
     7441 accgtccagg accaggagct cgtgcagagc atctgcctgc cgcccgagga cggcggcggt
     7501 gccccggcgc tccccggcct gcccggcgag ccggcgctcc cggccctgcc ctgacgcagc
     7561 cggcggaacg ggtccacgga tcggtgccgg cggtcgccgg tccgtgaccc accacctcgg
     7621 ggtcaggccg aaggtgccgg ttcgccccgc acgagggttc ggtacagatc ggcgtagagg
     7681 ccggcggcct ccagcagctc ctcgtggcgc ccggactcca cgacccggcc gtcgtcgacg
     7741 acgaggatct ggtcggcggt ggtgatggtg gagaggcggt gggcgatgac cagtgacgtc
     7801 cggccgacga gcgcctccgc gagggcggcc tgcaccgcgg cctcgttctc ggagtcgagg
     7861 tggctggtgg cctcgtcgag gatgacgatg gccgggtcct tgagcagcat gcgagcgatc
     7921 gacagccgct gcttctcgcc gcccgacatg cggtagccgc ggtcgccgac cacggtgtcg
     7981 tagccctcgg gcagggcagc gatgacgtgg tggatgcggg ctgcctcgca cgcctggcgc
     8041 agctcgtcga tcgtggcgtc gggccgcgcg tagcgcaggt tgcccgcgat ggtgtcgtgg
     8101 aacaggtggg ggtcctggct cacgacgccg atggcaagcc gcaggctctc ctgggtgagg
     8161 tctcggacgt cgtggccgtc gatgcggacg gcgcccgagt ccacgtcgta cagccggggg
     8221 agcagcgagg tgagggtggt cttgccggcg ccggacggcc ccacgatggc gaccagctgg
     8281 cccggatcga cgtgcgcgtc gatgcctcgc agcaccggac ccgacgaggc gtcgtggtcc
     8341 ggcacggcac cggcgaggga cggcaccgag acgtcctggg ctgtcgggta ggtgaaccag
     8401 acgtcgtcga agtcgacccg gccctgcacg tcggccaggt cctgcgcgcc cggacggtcc
     8461 tcgatcgggt tgcggatgtc gagcacctcg aacacccggt cgaaggagac gaacgccgtc
     8521 atgatgtcga tgcgggcgtt ggtgagggcg gtgagcggct ggtagatccg ctgcaccagg
     8581 gcggcgaggg ccacgagcgt gccgagggtg atggcgccgt cgatgacgag gatgccgccc
     8641 caccagtaca cggcggcgat gccgagcgca cccacgaggg cgatggcgga gaagaagacc
     8701 cgactgagca ccgcgctgcg caccccggcg tcgcgcacgc cggcggcgtc gtcggcgaag
     8761 cggtcggact cgtcgtcgtg gcggccgaag agcttgacga gcatcgcgcc ggccaccccg
     8821 aagcgctccg tcatggtcga gttcatccgg gcgttgcgct ccatctgctc ccggctgatg
     8881 acctgcagcc gggtgccgac ccgccgggcc gggatgagga aggcggggag cagggcgatg
     8941 gtgaacaggg tgaggcgcca ctcgagggcg accatcgccg cgaccgtcgt gacgagcacg
     9001 acgacgttgg agaccacgga gccgagcgtt ccggtgaggg cgcgctgggc accgatcacg
     9061 tcgttgttga gccgcgacac cagcgcgccc gtctgggccc gggtgaagaa ggcgaccggg
     9121 aggcgctgga cgtggtcgaa gacgcgcacc cgcaggtcga agatgagtcc ctcaccgatg
     9181 cgggacgaga gccaccgctc gaccagtgac agacccgagt cggccacggc ggcggcgacc
     9241 gccagcccgg ccaggaggga gagccgcccc ttgtcgccgt cggcgatggc gtcatcgagg
     9301 atccacttga acagcagcgg tggggcgagg ccgaggatgg cagccgtgat gatggcggcc
     9361 aggaagccga tgatctgcgt cttgtagggg cgggcgaact ccatgacccg ccgcacgatc
     9421 gagcgatcga gcgatgcgcc gtccaccgcg gaggcatcgc tcggcatcat cgagtggggg
     9481 ccctgtcgca cgggacgagg gtattggcct cgtggggtac ctcctccggc gcccgtcacc
     9541 cggtccgccc gggacgagat ctgctggcca gcggacagcc gctgaccagc agttcgtctg
     9601 gcggagggag tgggatttga acccacggag acccgaaggc ctcaacggtt ttcgagaccg
     9661 tcccattcgt ccgctctggc atccctcctg ggagctctgg agggtagcga acgggccgag
     9721 gcccctccca aggggtcagc ggcgctcggc gaagaacccg gcgagcagcg cgccgcactc
     9781 ctcggcccgc acgccggccc ggacgtcgag ctcgtggggc aggcgggggt cgacgccgag
     9841 gttgtagagc gagccgagcg caccggcctt ggggtccgcg gcgccgaaca cgacccggcc
     9901 gacgcgtgct gcgacgagcg cgcccgcgca catggggcag ggctcgagcg tcaccacgac
     9961 ctgggcgccg ggcaaccgcc aggagcctgc gtccgcgcac gccgcccgga gcgcgagcag
    10021 ctcggcgtgg gcgctggggt cgtggtcgcc ctggcggcgg ttgcgccccc gggccacgac
    10081 ctggccgccc accacgacca cggcgcccac cgggacctcg cccgcgtcgg ccgcgaggcg
    10141 tgcctcctcg agcgccaggc acatgagctc gtcgtcggtg ggggcggcgg gctccggcac
    10201 ggcggcgacg ttaccggcag ggggaggaag gccaccggtc gccgtcgaag gaccggggac
    10261 cgggtgtgcg agccggacgg tgctggggcc gtccggggcg cctccccgga cgcgccgatg
    10321 gcccgaccag cggccgggcc atcggtgctt cggc
//
LOCUS       DATISU010000376        11077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_869614, whole genome shotgun sequence.
ACCESSION   DATISU010000376 DATISU010000000
VERSION     DATISU010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 11077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11077
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869614"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..155
                     /locus_tag="VLR27_14830"
     CDS             6..155
                     /locus_tag="VLR27_14830"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04783.1"
                     /translation="MLAFSFIVTTILAKVLDATLGLRADEASETTGLDRTEHAESAYG
                     DALTA"
     gene            332..1003
                     /locus_tag="VLR27_14835"
     CDS             332..1003
                     /locus_tag="VLR27_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04784.1"
                     /translation="MADSKKPRAPFAPWDRRELPGAFSVEETAARVGQYKWIEMKLFE
                     ALGGWVATVPELDIKMRLGTHCYHHAWHADLWHKRLPELREMNPERLTGPASPEVEAF
                     MDAVAEPGEHDQTIEKLVGVYRVLIPHKVAAYTYHLNNTSTITDAPTIRSLKFALQDE
                     MEDWRDGTLMLTSLIQSDEEVQRAADHQAKLEKLLVAAGGICGPGTIGDAYDLSGDPA
                     RLTVA"
     gene            1043..1897
                     /locus_tag="VLR27_14840"
     CDS             1043..1897
                     /locus_tag="VLR27_14840"
                     /inference="COORDINATES: protein motif:HMM:NF016212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HSP04785.1"
                     /translation="MKKLLTPEELARDDRFERADMMDRIQDPRNGSEAANRGNGRLRP
                     ERADAPDAARALMHGIFVGEIQALEGAGRTCYDFEVGATGKLEDEEVPFELKLDMARQ
                     CWDEARHCEISVKLSEWMGTEIGEFGENAFLFQAACNPDPVLRLTGVNRALEGLAIDV
                     FNTMKEFGDLAGDPVLEFCEDWMLADEVTHVKMGSDWLRKLTVNDKDRQKRALEFQRV
                     VDKLFSFGGFRGEDEESPVHLARRFRELAGFDDAEMEEIAELSREAKSEVEERSRLAR
                     EANATASA"
     gene            1897..2463
                     /locus_tag="VLR27_14845"
     CDS             1897..2463
                     /locus_tag="VLR27_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04786.1"
                     /translation="MPTTVDPSTFTMVDFDSAQIAALADELAGKVGLGDDVQIVIEVD
                     ETTPLGRTELVSLDPVHIRTESGAFENPKRIRQFSEDATIDVLGRYLLRAADRRRDGF
                     GDVPPDSELSVPQRTAWEVSAVGRMARMGYPVRRQRWLYAFRNRYGFTDTADDAFEQL
                     WHGDDLTWAEIDRLSETTAATNPGPLQR"
     gene            complement(2609..3094)
                     /locus_tag="VLR27_14850"
     CDS             complement(2609..3094)
                     /locus_tag="VLR27_14850"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSP04787.1"
                     /translation="MTPERGATVHRPTRPLLAAVAAALLLLGAAACSDDDDAGAAAPA
                     AETSGTAVSAAGVRVVSPDEAATLLAADPAPSVIDVRTPEEFEEGHIEGAVLIDVTSP
                     DFAERVAELDRDQPYVLYCRSGNRSVTARQVLADLGFADVADIEGGITAWVAEGQPVV
                     T"
     gene            complement(3129..3449)
                     /locus_tag="VLR27_14855"
     CDS             complement(3129..3449)
                     /locus_tag="VLR27_14855"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HSP04788.1"
                     /translation="MSDEREVETAMVTMTFDATDPDRLLGVLSKYVVLTRGQDGCRNV
                     DLCLSVTMPDRFVIVQKWEHPEDQQRHFDSPEMVEMAAACTGLLATPPAIDLLEGISA
                     HDLA"
     gene            3532..4461
                     /locus_tag="VLR27_14860"
     CDS             3532..4461
                     /locus_tag="VLR27_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP04789.1"
                     /translation="MADRAGDDEDLVRLYLRDIGRHPLLSKDDEVHLAKLIEAGAEAR
                     AALEASSTISSTQRAALRRTARRGAAAQRRFIESNLRLVVSIAKRYQASGLPLLDLVQ
                     EGNLGLMHAVEKFDWRKGFKFSTYATWWIRQSISRGIANTGRTIRLPVHAGDQLVRVQ
                     RARARLETELGRPPTDAELAAETGFGIDKVREALRFAIEPLSLSEPLREDGEAELGDL
                     VEDVGASSPFDHAAATLLPSAVAQMLSGLEPRERDVLRLRYGLDRGDPRTLEEVGDAF
                     GLTRERIRQIEARALSKLRHPSIEAGARDLLAG"
     gene            complement(4516..4611)
                     /locus_tag="VLR27_14865"
     tRNA            complement(4516..4611)
                     /locus_tag="VLR27_14865"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4574..4576),aa:Sec,seq:tca)
     gene            complement(4675..5604)
                     /locus_tag="VLR27_14870"
     CDS             complement(4675..5604)
                     /locus_tag="VLR27_14870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HSP04790.1"
                     /translation="MEAAFFDLDKTVIARPSMVAFGAPLRREGLVSRRLLLRALWAQL
                     IYLTLGADEVRLERVRDAALRVSKGWEQRHISAIVRETITEVIEPIVYAEALELMAMH
                     REAGRKVFIVSASPEEIVAPLAQYLGADEAIATRAVLDEHGCYTGEVERYCYGPEKVR
                     VLEEVAERDGIDLDASYAYSDSATDAPMLAAVGHAIAVNPDRELLRLARTAGWEVVRF
                     SHRVRLRERVAFPSATTIGVGTVVASAVGGGAAVGWWLWNERQRRLAPVHVRGRRKAA
                     DALRRATEVVPALGPTRAGPSWRPAWPGTRVRR"
     gene            5755..6408
                     /locus_tag="VLR27_14875"
     CDS             5755..6408
                     /locus_tag="VLR27_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04791.1"
                     /translation="MLTAYWAAKGGAGATVLAAAHALVASTRRATVVVDLDGDLPAAL
                     GVEATDRPGVADWLRAGPDVPADALDRLLVPVADDLLLLPRGSGALDDGRAAVLGRLL
                     ADSPREVVVDAGTRPVGAARDVVRQADRSVLVTRACYLALRRLHGIGLAPTEVALVRE
                     ANRALGGADVTAAVGVPVRTTVGVDPGIARAVDAGLLATRLPRSLARAATAAGGIDR"
     gene            6408..7502
                     /locus_tag="VLR27_14880"
     CDS             6408..7502
                     /locus_tag="VLR27_14880"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HSP04792.1"
                     /translation="MDAAALDRLVHDEALAEGRPVSAAELGDLVHRHAPLLDAAQADA
                     AVRRVQARMHGLGVLDGLLGDETVTDVLVNGPGPVWVERHGQVEETEVVLDEPEVRRL
                     VERVVAPLGLRADRRNPLVDARLPDGSRVHVAMPPLAVDGPYLTVRRFSARTLPVAAF
                     AAVDVADLLIDAVRERRNLVVVGGTGAGKTTLLNALCQHLDPGERVVTVEDAAELRLP
                     GAHVVRLESRPASPDGLPAVTIRELVRTALRMRPDRIVVGEVRGGEALDMLQAMNTGH
                     EGSLSTCHANGPVQALRRLETMALTAGVDLPLRAVRDQLVSAVDLLVQVGRGPDGARR
                     VLAVDRVVDDPDASTRTEPLVVDGHLVAAP"
     gene            7499..8371
                     /locus_tag="VLR27_14885"
     CDS             7499..8371
                     /locus_tag="VLR27_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSP04793.1"
                     /translation="MSGEVAALAAGGAVLAASAGVTSARWWIAGARARHRLLERAASR
                     AAPMPGRVRDAFGRAGLAEEADRLLRLWLAGLFLTLTGSLLVPGVRLLLAGALVAGPV
                     GLLLATRRETRRRRAQLPEALDAVAAGLRSGLALPAAIAGAASVGAPLGDELGAMARD
                     VDAGRPLAEAIERWQAATPDDPLTGLAAAALTVATEVGGPGARAVDGAAASLRERLGS
                     EAETAALATQGRASAAVLTLAPLGFAFLLTSLDPAAGRFLLGTPVGWLCITIGLGLDG
                     LGAWWMARLVRRAR"
     gene            8368..9006
                     /locus_tag="VLR27_14890"
     CDS             8368..9006
                     /locus_tag="VLR27_14890"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSP04794.1"
                     /translation="MTALLATSCAVGAVLVVLHRPRRPARLVRPRPPRRRRRWTVPIR
                     RGRRAQLDDAQVARDLPDLIDLLALAVGAGLSVPAALPVVAPVAPVALRADLRSVVAA
                     MAGGRSSDEAVAELATCWGAPARPLLHAIADHLRYGTPVLPALERVAAEARSHRRRAA
                     EIRARKLPVLLLFPLVLCTLPAFGLLTVAPLVAGTFDSLRGGHLEAPASFGP"
     gene            9023..9271
                     /locus_tag="VLR27_14895"
     CDS             9023..9271
                     /locus_tag="VLR27_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04795.1"
                     /translation="MHPISLVLQLHVVTAIGAVAGRARGLLARAGTGRERGQSTAEYA
                     LVLLGAATVAIVFLAWAGKTSRIGKLFDAVLDTVIGKV"
     gene            9268..9666
                     /locus_tag="VLR27_14900"
     CDS             9268..9666
                     /locus_tag="VLR27_14900"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HSP04796.1"
                     /translation="MTGAVRSTRPGHGAGHRGQATVELALGLPVVCIGVLLVLQLALV
                     GRDAVLVTHAAREAARAAAVEPTGPAARTGAVAASSALDPSRLDVRLRRSGGRVTVEV
                     GYRSITDLPLVGALIPDPDLHGQATMLEEG"
     gene            9825..11027
                     /locus_tag="VLR27_14905"
     CDS             9825..11027
                     /locus_tag="VLR27_14905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HSP04797.1"
                     /translation="MNVRRMMGITALAAAATGLVFVPGAGAQEAGPSYGGSSSGEALN
                     LSLFGQGLTVGSTLAELTPTSGTAQGSGVATPAFEAGATSASETAPGSADETCEGPLD
                     QIPGISIALACSSSQVTMGETSTAAATGRVGTITLNPVAPILDTPLSEVVAPVEDGVD
                     QLLGGLEPVLGPIDEASDLGLEDTLGELFDRLFAGADLATVTLGDTASTTTANATNVT
                     TSCVAEGARVDVLSVLDGPPLISVVVGRAGSTVDVDRASGTGTPTVDPALVTIESATL
                     GVTPITVPVGQTVEVPLPEPFGPSYIRVADGTTGTNEDGSTFATANAVSLDLLNGEAL
                     MGGIELELAGCTSVAGAEVPVQPAAAAPVPTLPTTGSDDTGMLVLAGLAGLAGLGLAL
                     LRRSGTDT"
BASE COUNT         1446 a         4321 c         3865 g         1445 t
ORIGIN      
        1 tcgtggtgct ggccttctcc ttcatcgtca cgacgatcct ggccaaggtg ctcgacgcca
       61 ccctggggct ccgggccgac gaggcgtcgg agaccaccgg cctcgaccgc accgagcacg
      121 ccgagagcgc ctacggcgac gccctcaccg cctgacccgg gagggacgaa cgaaccgatc
      181 ggtcccgacg ttccactgga tggaacgacc cgaccgatcg gttcgttcgt gtcggcctcg
      241 attcccaggt gcgcccttga ccgatgggtc aggtgacgta gggtgggata gcgatcgtta
      301 tcgcaccagt ggccgagtca cagggggaca cgtggccgat tcgaagaagc cgagagcgcc
      361 gttcgcaccg tgggaccgcc gggagctgcc cggcgcgttc agcgtcgagg agacggcggc
      421 ccgggtgggt cagtacaagt ggatcgagat gaagctgttc gaggcgctgg gcggctgggt
      481 cgccaccgtg cccgagctcg acatcaagat gcgcctcggc acgcactgct accaccacgc
      541 gtggcacgcc gacctgtggc acaagcgcct gcccgagctg cgggagatga accccgagcg
      601 cctgaccggg ccggccagcc cggaggtcga ggcgttcatg gacgccgtcg ccgaaccggg
      661 cgagcacgac cagaccatcg agaagctggt cggggtgtac cgggtgctca tcccccacaa
      721 ggtggcggcg tacacgtacc acctgaacaa cacctccacg atcaccgatg cacccaccat
      781 ccggtccctg aagttcgcgc tccaggacga gatggaggac tggcgcgacg gcacgctgat
      841 gctgacctcg ctcatccagt ccgacgagga ggtccagcgc gctgcggacc accaggccaa
      901 gctcgagaag ctgctcgtcg ccgccggggg gatctgcggc ccgggcacca tcggcgacgc
      961 ctacgacctc tccggcgacc cggctcgcct caccgtcgcg taggcgacgg cacaccaact
     1021 cgaacccagg gggacacaca ccatgaagaa gctgctcaca cccgaagagc tggcccgtga
     1081 cgaccgcttc gagcgggccg acatgatgga ccggatccag gacccgcgga acggcagcga
     1141 ggccgccaac cgcggcaacg gccggctccg tcccgagcgc gccgacgcgc ccgacgccgc
     1201 ccgcgccctg atgcacggca tcttcgtggg cgagatccag gccctcgagg gtgccggtcg
     1261 cacctgctac gacttcgagg tcggggcgac ggggaagctg gaggacgaag aggtcccctt
     1321 cgagctcaag ctcgacatgg cccgccagtg ctgggacgag gcccgccact gcgagatctc
     1381 cgtgaagctc agcgagtgga tgggcaccga gatcggtgag ttcggcgaga acgccttcct
     1441 gttccaggcc gcctgcaacc ccgacccggt cctgcgcctc accggcgtga accgcgccct
     1501 cgagggcctg gccatcgacg tcttcaacac catgaaggag ttcggcgacc tggccggcga
     1561 tccggtgctg gagttctgcg aggactggat gctcgccgac gaggtcaccc acgtgaagat
     1621 gggctcggac tggctccgca agctcaccgt gaacgacaag gaccgccaga agcgggccct
     1681 cgagttccag cgggtcgtcg acaagctgtt ctccttcggc ggtttccggg gcgaggacga
     1741 ggagagcccg gtccacctgg ctcgccgctt ccgcgagctc gccggcttcg acgacgccga
     1801 gatggaggag atcgccgagc tgtcgcgcga ggccaagtcc gaggtcgagg agcgctcgcg
     1861 cctggcccgc gaggcgaacg cgacggcgag cgcctgatgc ccacgacggt cgacccgtcg
     1921 accttcacca tggtcgactt cgacagcgcg cagatcgccg cgctcgccga cgagctggcc
     1981 ggcaaggtgg gcctgggcga cgacgtgcag atcgtcatcg aggtcgacga gacgacgccg
     2041 ctcgggcgca ccgagctggt gtcgctggac ccggtgcaca tccgcacgga gtcgggggcg
     2101 ttcgagaacc cgaagcgcat ccgccagttc agcgaggacg caaccatcga cgtgctcggc
     2161 cgctacctgt tgcgagccgc cgatcgtcgg cgcgacggct tcggcgacgt gccgccggac
     2221 agcgagctct ccgtgccgca gcgcacggcg tgggaggtgt ccgccgtcgg tcgcatggcc
     2281 cgcatggggt acccggtgcg ccggcagcgg tggctctacg cgttccgcaa ccgctacggc
     2341 ttcaccgaca ccgccgacga cgccttcgag cagctctggc acggggacga cctcacctgg
     2401 gccgagatcg accggctcag cgagaccacc gccgcgacca acccgggccc gctccaacgc
     2461 tgacaccgtg ccggtgcacg agctgcgcac cgacggctcg tgctgcgcca gtggcgggag
     2521 tcggatcgcg cgccgtgggc ggcggtcaac gccgacccgg aggtgatggc gcacttccac
     2581 tcgaccctgt ccctggaggc gcggccggtc acgtgacgac cggctggccc tccgcgaccc
     2641 aggcggtgat gccgccctcg atgtcggcga cgtcggcgaa cccgaggtcg gccagcacct
     2701 ggcgcgccgt gacggaccgg ttgcccgacc ggcagtacag gacgtagggc tggtcgcgat
     2761 cgagctcggc gacccgttcg gcgaagtccg ggctggtgac gtcgatgagc accgcgccct
     2821 cgatgtggcc ctcctcgaac tcctccgggg tgcgcacgtc gatgaccgat ggagccgggt
     2881 cggcggccag gagcgtggcg gcctcgtccg gcgacacgac gcgcacgccc gcggcggaca
     2941 cggcggtgcc ggaggtctcc gcggccgggg ccgcggcccc ggcatcgtcg tcgtcactgc
     3001 acgcggcggc gccgagcagg aggagggcag ccgcgacagc ggccaggagg gggcgggtgg
     3061 ggcggtgcac ggtggctcct cgttcggggg tcatccgcag ggtagcgaga tgtccggaca
     3121 actggcgttc aggccaggtc gtgcgcgctg atcccctcga gcaggtcgat ggccggcggc
     3181 gtcgccagca acccggtgca ggccgcggcc atctccacca tctcgggcga gtcgaagtgc
     3241 cgctgctggt cctcggggtg ctcccacttc tgcacgatca cgaagcggtc gggcatggtg
     3301 accgacaggc acaggtcgac gttgcggcac ccgtcctggc cccgggtgag caccacgtac
     3361 ttcgacagca cgccgagcag gcggtccgga tcggtggcat cgaaggtcat ggtcaccatc
     3421 gcggtctcga cctcacgctc gtcgctcacg tgccgtgctc ctcacccctg gtccggatca
     3481 cgggacagta gtaccgttcc ttcaccgcag ctccggggga caggagaggt catggcggac
     3541 cgtgcaggag acgacgagga cctcgtccgg ctctacctgc gcgacatcgg ccgccacccg
     3601 ctgctgagca aggacgacga ggtccacctg gccaagctca tcgaggccgg cgcagaggca
     3661 cgggccgcgc tcgaggcgtc gagcacgatc tcctcgaccc aacgggccgc gctgcgccgc
     3721 acggccaggc gcggcgccgc ggcgcagcgg cgcttcatcg agtcgaacct gcgactggtc
     3781 gtgtcgatcg ccaagcgcta ccaggcgtcc ggcctgccgc tgctcgacct ggtgcaggag
     3841 gggaacctcg gcctcatgca cgccgtcgag aagttcgact ggcgcaaggg gttcaagttc
     3901 tcgacctacg ccacgtggtg gatccgccag tccatcagcc ggggcatcgc caacaccgga
     3961 cgcaccatcc ggctccccgt ccacgccggg gaccagctgg tgcgcgtgca gcgcgcccgc
     4021 gcccgcctgg agaccgagct cggacgcccg ccgaccgatg ccgagctcgc cgccgagacc
     4081 ggcttcggca tcgacaaggt ccgcgaggcg ctgcgcttcg ccatcgagcc gctctcgctg
     4141 tcggagccgc tgcgggagga cggcgaggcc gagctcggcg acctggtgga ggacgtcggc
     4201 gcgtcctccc ccttcgacca cgccgccgcg acgctgctgc ccagcgccgt cgcgcagatg
     4261 ctcagcgggc tcgagccgcg cgagcgcgac gtcctccgcc tgcgctacgg cctcgaccgc
     4321 ggcgacccgc gcaccctcga ggaggtcggc gacgcgttcg ggctcacccg ggagcgcatc
     4381 cgccagatcg aggcgcgggc gctctccaag ctgcgccacc cgagcatcga ggccggggcg
     4441 cgcgacctgc tcgccggctg aggagccgat cctgaacgtg gcggtggggc ctcagcgcga
     4501 ggccccaccg ctcggtggcg gaggtggacg ggaatcgaac ccgccgcacg gggagcgccc
     4561 gtgccaaccg ctttgaaggc ggcggagccc accaggtgct cggacacctc cgctcggcaa
     4621 cctagttctc cgcccgtggg gcctagtctc ggccgtccat gaagaagctc ctgctcatcg
     4681 ccgcactcgt gttcctggcc aggctggccg ccaagaaggc ccagcacgtg taggcccgag
     4741 cgccggcacc acctcggtcg cccggcgcag ggcgtccgcc gccttgcgac gcccccgcac
     4801 gtgcaccggc gcgagccggc gctggcgctc gttccagagc caccacccca cggccgcgcc
     4861 accacccacc gcggacgcga ccacggtgcc gacgccgatg gtggtggccg acgggaacgc
     4921 gacccgctcg cgcagccgga cccggtggga gaagcggacg acctcccacc cggcggtgcg
     4981 ggcgaggcgc agcagctccc ggtcggggtt cacggcgatg gcgtgcccga cggcggcgag
     5041 catcggggcg tcggtggccg agtcggagta ggcgtaggac gcgtcgaggt cgatgccgtc
     5101 gcgctcggcc acctcctcca gcacccggac cttctcgggg ccgtagcagt accgctcgac
     5161 ctcgcccgtg tagcagccgt gctcgtcgag gaccgcccgg gtggcgatcg cctcgtcggc
     5221 cccgaggtac tgggccaggg gggcgacgat ctcctccggt gaggccgaca cgatgaacac
     5281 cttgcgcccc gcctctcggt gcatggccat gagctcgagc gcctcggcgt agacgatggg
     5341 ctcgatgacc tcggtgatcg tctcgcgcac gatcgccgag atgtggcgct gctcccaccc
     5401 cttggagacc cggagcgccg cgtcgcggac ccgctccagg cggacctcgt cggcaccgag
     5461 ggtcaggtag atcagctggg cccacagcgc ccgcagcagc aggcggcgcg agaccagccc
     5521 ctcccggcgc agcggcgcgc cgaacgcgac catcgacgga cgggcgatca ccgtcttgtc
     5581 caggtcgaag aaggccgctt ccacgagcgg gagcctacgg aaggccacgg accggaccgg
     5641 ttgcggatct tcgatgccct tggtacgttc tcggccactc tccatccggc aaccgccggg
     5701 agagcccggc ttcccccgcc acctctgcgc cacgccgcgc gcgaagggga tcccgtgctc
     5761 accgcctact gggccgcgaa gggtggcgcc ggcgccaccg tgctcgccgc cgcccatgcc
     5821 ctcgtcgcct cgacccgccg cgccaccgtc gtcgtggacc tcgacggcga cctgcccgcc
     5881 gccctcgggg tcgaggccac ggatcggccg ggcgtggccg actggctacg ggccggcccc
     5941 gacgtgcccg ccgacgccct ggatcggctg ctcgtcccgg tcgccgacga cctcctgctc
     6001 ctcccccgtg gctccggtgc gctcgacgac ggacgggccg ccgtcctcgg ccgcctgctg
     6061 gccgacagcc cacgcgaggt ggtggtcgac gccggcaccc ggcccgtagg cgcggctcgt
     6121 gacgtcgtgc gccaggccga tcgcagcgtg ctcgtcaccc gggcctgcta cctcgccctg
     6181 cgccggctgc acggcatcgg cctggccccc accgaggtcg ccctcgtccg cgaggccaac
     6241 cgcgccctcg gcggcgccga cgtcaccgcc gccgtcggcg tgcccgtgcg caccacggtc
     6301 ggggtcgatc ccggcatcgc ccgggccgtg gacgccggcc tcctcgccac ccgcctcccc
     6361 cgcagcctgg ctcgggcggc gacggccgcc ggcgggatcg accgctgatg gacgccgctg
     6421 cactggaccg gctcgtccac gacgaggccc tggccgaggg ccggccggtc agcgccgccg
     6481 agctcggcga cctcgtgcac cgccacgccc cgctgctcga cgcggcgcag gcggacgccg
     6541 cggtgcgccg ggtccaggcc cgcatgcacg gcctcggcgt cctcgacggc ctgctcggcg
     6601 acgagacggt caccgacgtg ctggtcaacg gtcccggccc ggtgtgggtc gaacggcacg
     6661 gccaggtcga ggagaccgag gtcgtcctcg acgaacccga ggtgcgccgc ctggtcgagc
     6721 gcgtggtggc gcccctcggc ttgcgcgccg accgccgcaa ccccttggtc gacgcgcggc
     6781 tgcccgacgg ctcccgcgtc cacgtggcca tgcccccgct cgccgtcgac ggcccgtacc
     6841 tgaccgtgcg ccgcttcagc gcccgcaccc tcccggtggc ggcgttcgcc gcggtcgacg
     6901 tggccgacct cctgatcgac gccgtccgcg agcggcgcaa cctggtggtc gtcggcggca
     6961 ccggcgccgg gaagacgacg ctgctcaacg cgctgtgcca gcacctcgac cccggcgagc
     7021 gcgtcgtcac cgtcgaggac gcggccgagc tgcggctccc cggcgcgcac gtggtgcggc
     7081 tcgagagccg tccggcgtcg cccgacgggc tgcccgccgt caccatccgc gagctggtgc
     7141 gcaccgcgct gcgcatgcga cccgatcgca tcgtggtcgg cgaggtccgg ggtggggagg
     7201 cgctcgacat gctccaggcg atgaacaccg ggcacgaggg ctcgctgtcc acctgccacg
     7261 ccaacggtcc ggtgcaggcg ctgcggcggc tggagacgat ggcgctcacc gccggcgtcg
     7321 acctgccgct ccgggcggtg cgggaccagc tcgtctcggc cgtcgacctg ctcgtgcagg
     7381 tcggccgggg tccggacgga gcacggcggg tcctggccgt cgaccgcgtc gtcgacgacc
     7441 ccgacgccag cacccgcacc gagccgctgg tcgtcgacgg gcacctggtg gcggcgccgt
     7501 gagcggcgag gtcgccgccc tcgccgccgg cggcgccgtc ctggcggcat cggccggcgt
     7561 gacgagcgcc aggtggtgga tcgccggcgc ccgcgcccgc caccgcctcc tcgagcgcgc
     7621 cgcgtcccgg gcggcgccca tgcccggccg ggtccgggac gcgttcggtc gcgccggcct
     7681 ggccgaggag gccgatcgcc tgctccgcct gtggctcgcc ggtctgttcc tcaccctcac
     7741 cggctcgctg ctggtgccgg gagtgcggct cctcctggcc ggggcgctcg tcgccgggcc
     7801 ggtcggcctc ctcctcgcca cccggcgcga gacccgacgt cgccgcgcgc agctccccga
     7861 ggcgctcgac gccgtcgccg ccgggctgcg cagcggcctc gccctgccgg ccgccatcgc
     7921 cggggcggcg tccgtcgggg cacccctcgg cgacgagctc ggcgccatgg cccgcgacgt
     7981 cgacgcgggc cgccccctgg ccgaggccat cgaacgctgg caggccgcca cccccgacga
     8041 tccgctcacc ggtctggccg cagccgccct caccgtggcc accgaggtcg gagggccggg
     8101 cgcacgggcg gtcgacgggg cggcggccag cctgcgggag cggctcggca gcgaagccga
     8161 gacggcggcg ctggccaccc agggccgggc ctcggccgcg gtgctcaccc tcgcgccgct
     8221 gggcttcgcc ttcctgctca cgtcgctcga tccggccgcc ggccggttcc tgctcggcac
     8281 cccggtgggg tggctctgca tcacgatcgg cctgggcctc gacggtctcg gtgcctggtg
     8341 gatggcccgg ctggtccggc gggcccgatg accgccctgc tcgccaccag ctgtgccgtc
     8401 ggcgccgtgc tcgtcgtcct ccaccggccg cgccgccccg cccggctggt ccgcccccgt
     8461 ccgccgcggc gccgccggcg ctggaccgtc ccgatccgac gtgggcggcg cgcccagctc
     8521 gacgacgccc aggtggcacg agacctcccc gatctcatcg atctgctcgc cctcgccgtc
     8581 ggtgccggcc tctccgtgcc ggccgccctg ccggtcgtcg cacccgtcgc accagtcgcc
     8641 ctccgagccg acctccgatc cgtcgtcgcg gccatggccg gtggccggtc gtccgacgag
     8701 gccgtcgccg agctcgcgac ctgctggggc gcgcccgccc gaccgctcct ccacgccatc
     8761 gccgaccacc tccgctacgg cacgcccgtc ctgccggccc tggagcgggt cgcggccgag
     8821 gcccggagcc accgtcggcg cgccgccgag atccgggccc ggaagctgcc ggtcctcctg
     8881 ctcttcccgc tcgtgctgtg caccctgccg gccttcggcc tgctcacggt cgctccgctc
     8941 gtggcgggca ccttcgactc cctccgcggg ggtcacctcg aggccccggc ctccttcggt
     9001 ccctgaacgt caggagtcct ccatgcatcc catctccctc gtgctccagc tgcacgtggt
     9061 caccgcgatc ggcgccgtcg ccggtcgcgc ccgcggcctg ctggcccgag ccggcaccgg
     9121 ccgcgagcgc ggccagtcca ccgccgagta cgccctcgtg ctgctcggcg ccgccaccgt
     9181 cgccatcgtc ttcctggcct gggccggcaa gacgagccgc atcggcaagc tcttcgacgc
     9241 cgtgctcgac acggtgatcg ggaaggtgtg accggcgcag tccgctcgac ccggcctggt
     9301 cacggtgcag gtcaccgtgg ccaggccacc gtcgagctcg ccctcggcct ccccgtcgtg
     9361 tgcatcggcg tgctcctggt cctgcagctc gcgctcgtgg ggcgcgacgc cgtgctcgtc
     9421 acccacgccg cccgggaggc cgctcgtgcc gccgcggtcg agccgacggg tcccgccgcc
     9481 cgcaccgggg cggtcgcggc ctcctcggca ctcgacccgt cgcggctcga cgtgcggctg
     9541 cgccgcagcg ggggacgggt caccgtcgag gtcgggtacc ggtcgatcac cgacctgccc
     9601 ctcgtcggcg ccctcatccc cgacccggac ctgcacggcc aggccacgat gctcgaggag
     9661 ggctgacgat ctgctccggt ggcggctcgg cacctccccc tggggcagac tggcccgacc
     9721 tcgggaggaa ggacttcctg acgaccgggg cgaacactgg ggagtccgag cccggacttg
     9781 cagcaagacg tgacgcgctc gccgcgtcgg ccgtggaggt cgtcatgaac gttcgacgaa
     9841 tgatggggat caccgccctg gctgcggccg ccaccggact ggtcttcgtg ccgggcgcgg
     9901 gcgcccagga ggccggaccg tcctacggcg ggtcgtcctc gggcgaagcg ctgaacctgt
     9961 ccctcttcgg ccaggggctc accgtgggat ccacgctcgc cgagctcacc cccacctcgg
    10021 gcaccgccca gggcagcggc gtggccaccc cggccttcga ggccggcgcc acctcggcgt
    10081 cggagaccgc tcccggctcg gccgacgaga cgtgcgaggg cccgctcgac cagatcccgg
    10141 gcatcagcat cgccctcgcc tgcagctcgt cccaggtcac catgggcgag acctccaccg
    10201 ccgcggccac cggccgggtc ggcaccatca cgctcaaccc cgtcgccccc atcctcgaca
    10261 ccccgctgtc cgaggtcgtc gctccggtcg aggacggcgt ggaccagctc ctcggcggcc
    10321 tggagcccgt gctcggcccg atcgacgagg ccagcgacct cggcctcgag gacaccctgg
    10381 gcgagctgtt cgaccgcctc ttcgccgggg ccgacctcgc caccgtcacc ctcggcgaca
    10441 ccgcctccac cacgaccgcg aacgccacga acgtgacgac ctcctgcgtc gccgagggcg
    10501 cccgggtcga cgtgctcagc gtcctcgacg gcccgccgct gatctcggtc gtcgtcggcc
    10561 gggccggctc caccgtcgac gtcgaccgtg ccagcggcac cggcaccccg acggtcgacc
    10621 cggcgctggt caccatcgag tcggcgacgc tcggcgtcac ccccatcacg gtgcccgtcg
    10681 gccagaccgt cgaggtcccc cttcccgagc cgttcggccc ctcctacatc cgggtcgccg
    10741 acggcaccac cggcacgaac gaggacggct ccaccttcgc cacggccaac gccgtcagcc
    10801 tcgacctgct caacggcgag gccctcatgg gcggcatcga gctggagctc gccgggtgca
    10861 catcggtcgc cggcgccgag gtgccggtcc agcccgcggc ggcggcgccg gtccccaccc
    10921 tgccgaccac cgggtccgac gacaccggca tgctcgtcct cgccggtctc gccggcctcg
    10981 ccggcctcgg tctggcgctg ctgcgacgct ccggcaccga cacctgacgc ccccgaccac
    11041 cagccgctgc tgatgcacct cctcgccgac cccgcca
//
LOCUS       DATISU010000377         5717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_728842, whole genome shotgun sequence.
ACCESSION   DATISU010000377 DATISU010000000
VERSION     DATISU010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728842"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VLR27_14910"
     CDS             complement(<1..621)
                     /locus_tag="VLR27_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HSP04798.1"
                     /translation="MADFRELLAATKAQIREIDTEEADRLRRDGDGVVILDVREPDEN
                     AQGSIPGAVFIPRGQLESNIENKIPNKDATVLIHCASGVRSAFAAKTLAELGYGDVAS
                     VAGGFNKWKDEGREWSTPKNLTPEQRNRYQRHLLLPEVDIEGQIKLLEAKVLLLGAGG
                     LGSPAALYLAAAGVGTLGIIDMDVVDESNLQRQILHNMDRIGMRKVD"
     gene            complement(708..1421)
                     /locus_tag="VLR27_14915"
     CDS             complement(708..1421)
                     /locus_tag="VLR27_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP04799.1"
                     /translation="MTVGGDRASVLVVEDDADIRDVLARLLDRDGFEPHACATGADAV
                     ASLGAVDPEVVLLDLGLPDIDGFDLLRTIRSRSDATVIVLSGWASESDRVLALELGAD
                     DYVVKPFLTRELVARIRAHRRRATATTADRSDGLLVEGDLQIDQRTREVTVAGEAVDL
                     TAREFDLLHFLASSPRQVFSRDQILEHAWGSSVVWQQDATVTEHVHRLRQKVGAEHIA
                     TVRSVGYRFEPQVAAHVAE"
     gene            1573..3921
                     /locus_tag="VLR27_14920"
     CDS             1573..3921
                     /locus_tag="VLR27_14920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP04800.1"
                     /translation="MRRRSTLAPLAALAIGVTVSVVAGRDVQQTAERELAISLDRGAS
                     EIGMMIQSLAFGLEAELSSLAAVATVTGGDPLLQQTFVEDQGLDGGYVLVDASGEDLR
                     VAAVAAPDEATTAAQRALAEAFIASPGPATELRQLVDGSSFGFVLGGDAAQTLALAAG
                     TVAGDGSAYAALRLFDVGDAGLFVSEPMAGAERFAVYAGDVPDPSRAVLATADPLPLT
                     GTTTSLEAAVGEQSLLIEVGGEPVHAISPAVVVGTGIALTLLLAGLLSTALRRRDAAL
                     RALAAAERADQQFRAALLASPDLILRWDVEHDELEVLNRDEFYGYDATRTHRVDDLLE
                     LIVSEDRDRVRTLAGSAGTWADELDGEDGSDIRIRAADGTHHWVHLRVGRMQPERADG
                     DLLIVVTDVDDVHRAAEQRSAVEAELQQAQRMESIGQLAGGIAHDFNNLLAAIAATAE
                     LVLDDVDDPATMADVEAILDATQRGSGLTKQLLSFSRRGLTRREEVEVDAVVRGMADL
                     LRRTIGEHIELQVACGAPGAHVLGDPAELEQILLNLAVNARDAADGTGLQITVRTEVA
                     GGQVALSVEDNGAGMSADVVDRAFEPFFSTKPTDRGTGLGLSIVYGIATRMGGSVGID
                     STEGRGTTVRLELPCTETSPSPAGLTRPVEAGVAAHESVLLVEDEPAVRRAAQRLLER
                     AGHAVVAAADGHEAVDAVLDGLRPTVLLTDVVLPGTLTGHDVAERVRAVIPDVRIVYA
                     SGYSRDVLTAEQLESEHARFVSKPFTSRSLLDAVAGAVEVVA"
     gene            3918..4973
                     /locus_tag="VLR27_14925"
     CDS             3918..4973
                     /locus_tag="VLR27_14925"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSP04801.1"
                     /translation="MTGLLVVEDEPTLARALGRVFADHFDHVHVAGTAREARAVLAQH
                     RHVIDVVFTDVMLPDGDGITLVAELRQLDPTLGAVVATGVDDPLTAERAVELGVQGYL
                     LKPFEMTEARINAANAQHWRRLEQENRDHRERLSSLVDVRTRALERSRSETITRLSVA
                     AEHRDMETSSHLERMSAYAAILARRAGLDPEHCEQIRLASPMHDIGKLGIPDHVLLHD
                     GPFTPEQRRIMGTHTVLGHRILAGSESPLLELGATIALTHHEWWDGSGYPCGLAGTDI
                     PLEGRIVAIADVFDALASPRRYKRAFGVDEAVEIMRSERGTHFDPWLLDRFLADLDEL
                     LFIRSAHPDRADVVYAG"
     gene            complement(4999..5436)
                     /locus_tag="VLR27_14930"
     CDS             complement(4999..5436)
                     /locus_tag="VLR27_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04802.1"
                     /translation="MDLDKLSLSDKIIAGTGIVLIIDLLFLPWHSIDVGPFSASLTAI
                     EEENAFLGVLALLLTIVVVAVTLLRVFKPETKLPDLPVTWDRAIFFGAAATLGLLVLK
                     LILETDYLGIGAWLGLLLAAGMTYGGYLKFQADSTASAGTGTV"
     gene            complement(5529..>5717)
                     /locus_tag="VLR27_14935"
     CDS             complement(5529..>5717)
                     /locus_tag="VLR27_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HSP04803.1"
                     /translation="RADLLLAVGSTLGVYPIANVVPLAKVHGASVLIVNGGPTEMDDL
                     ADVRIEGSISEVLPALLG"
BASE COUNT          810 a         2046 c         2045 g          816 t
ORIGIN      
        1 gtcgaccttg cgcatgccga tgcggtccat gttgtggagg atctggcgct gcaggttgga
       61 ctcgtcgacg acgtccatgt cgatgatgcc gagcgtgccc acgccggcgg cggccaggta
      121 gagcgcggcc ggggagccga gaccgccggc gccgaggagc aggaccttgg cctcgagcag
      181 cttgatctgg ccctcgatgt cgacctcggg cagcaggagg tggcgctggt agcggttgcg
      241 ctgctcgggc gtgaggttct tcggggtgct ccactcgcgc ccctcgtcct tccacttgtt
      301 gaacccgccg gccacggagg cgacgtcgcc gtagcccagc tcggccaggg tctttgccgc
      361 gaaggcggag cgcacaccgg aggcgcagtg gatcaggacc gtggcgtcct tgttcgggat
      421 cttgttctcg atgttggact ccagctggcc ccgggggatg aagaccgcgc cggggatgga
      481 gccctgggcg ttctcgtccg gctcacggac gtcgaggatg acgacgccgt cgccgtcgcg
      541 gcggaggcgg tcggcctcct cggtgtcgat ctctcggatc tgggccttgg tggcagcgag
      601 gagctcgcgg aagtcggcca tcggtgttcg ccttcgtgct ggtggaccgg tcggaggagg
      661 gatccgtgaa tccctactca gtaggtcaac aattgcgcgg cgcggcccta ttccgcaacg
      721 tgggcggcga cctggggctc gaagcggtac ccgacgctgc gcaccgtggc gatgtgctcg
      781 gcccccacct tctgtcggag ccggtggacg tgctcggtga cggtggcgtc ctgctgccac
      841 acgacggacg acccccaggc gtgctcgagg atctggtcgc ggctgaacac ctgccggggt
      901 gaggacgcga ggaagtgcag caggtcgaac tcacgagcgg tcaggtccac cgcttcgccg
      961 gccaccgtga cctcccgcgt gcgctggtcg atctgcaggt caccctcgac gaggagcccg
     1021 tcggagcggt ccgcagtggt ggcggtggct cgacggcggt gcgcgcggat gcgggcgacc
     1081 agctcgcggg tgaggaaggg cttgaccacg tagtcgtcgg cgccgagctc cagggccagc
     1141 acccgatcgc tctccgatgc ccagcccgac agcacgatga cggtggcgtc gctgcggctg
     1201 cggatggtgc ggaggaggtc gaagccgtcg atgtcgggca gaccgaggtc gagcagcacg
     1261 acctcggggt cgacggcgcc gagggacgcg acggcatcgg caccggtcgc gcaggcgtgg
     1321 ggctcgaagc cgtcccggtc cagcagcctc gccagcacgt cgcgaatgtc tgcgtcgtcc
     1381 tccacgacca gcacgctcgc ccggtccccg cccaccgtca tgtccgaaat ggtgccagat
     1441 gggcgcttcg ccgcgtcggc agcgttgagc gagcgttgag gaacgcccga gggtccggtg
     1501 actcgcaccc cgcagtgttc gcaccatgac cacggaactc ctcgccacga ccctggatcg
     1561 gggcgtctcc tgatgcgccg ccgcagcacg ctggctcccc tcgccgcgct cgccatcggc
     1621 gtcacggtga gcgtggtcgc cggccgcgac gtgcagcaga ccgccgagcg cgagctcgcc
     1681 atctccctcg atcggggtgc gagcgagatc ggcatgatga tccagagcct cgccttcggg
     1741 ctcgaagccg agctctcctc gctcgccgcc gtcgccaccg tcaccggcgg cgacccgctc
     1801 ctgcagcaga ccttcgtgga ggaccagggc ctcgacggcg ggtacgtgct cgtcgacgca
     1861 tccggagagg acctgcgggt ggcggccgtc gccgcccccg acgaagccac gaccgctgcg
     1921 cagcgcgccc tggccgaggc cttcatcgcc tccccgggac cggcgaccga gctgcgccag
     1981 ctggtcgacg ggtcctcctt cgggttcgtg ctcggcggcg acgccgccca gacgctggcg
     2041 ctggccgccg gcaccgtcgc cggcgacggc tccgcctacg ccgcgctgcg actcttcgac
     2101 gtcggcgacg cagggctgtt cgtctcggag ccgatggccg gcgccgagcg cttcgccgtc
     2161 tacgccggcg acgtgcccga cccgtcccga gccgtgttgg ccaccgccga ccccctcccg
     2221 ctcaccggca ccacgacgtc gctcgaagcc gcggtcggcg agcagtcgct gctcatcgag
     2281 gtggggggcg aacccgtcca cgccatctcg cccgccgtcg tcgtcggcac cggcatcgcc
     2341 ctcacgctgc tgctggccgg cctcctctcc acggcgctgc gccgccggga cgcggcgctg
     2401 cgggccctgg cggctgccga gcgggccgac cagcagttcc gtgccgcgct gctcgcctcg
     2461 cccgacctga tcctgcgctg ggatgtggag cacgacgagc tcgaggtgct gaaccgggac
     2521 gagttctacg gctacgacgc cacccgcacc caccgggtcg atgacctgct cgagctgatc
     2581 gtgtccgagg accgggatcg ggtgcgcacc ttggccgggt cggctgggac gtgggccgac
     2641 gagctcgacg gcgaggacgg cagcgacatc cggatccggg cggcggacgg cacccaccac
     2701 tgggtccacc tgcgcgtggg tcggatgcag cccgagcgcg cggatggcga cctgctgatc
     2761 gtggtgaccg acgtcgacga cgtgcaccgg gcggccgagc agcgctcggc tgtggaggcc
     2821 gagctccagc aggcccagcg catggagtcg atcggccagc tcgccggcgg gatcgcccac
     2881 gacttcaaca acctgctggc ggccatcgcc gcgacggccg agctcgtgct cgacgacgtc
     2941 gacgacccgg cgacgatggc ggacgtggaa gcgatcctcg acgccaccca gcgcggctcg
     3001 gggctgacca agcagctgct ctccttcagc cggcggggtc tgacccggcg cgaagaggtc
     3061 gaggtcgatg ccgtcgtccg cggcatggcg gacctgcttc gccggacgat cggcgagcac
     3121 atcgagctgc aggtggcgtg cggcgctccg ggggcgcacg tcctcggcga cccggccgag
     3181 ctcgagcaga tcctgctcaa cctggccgtc aacgcccggg acgccgccga cgggaccggg
     3241 ctccagatca ccgtgcgcac cgaggtcgcc ggtggccagg tggcgttgag cgtcgaggac
     3301 aacggtgcag gcatgagcgc cgacgtcgtc gaccgggcgt tcgagccgtt cttctccacg
     3361 aagcccaccg accggggtac cgggctcggc ctgtccatcg tctacggcat cgccacccgc
     3421 atgggcggtt ccgtgggcat cgactccacc gaggggcgcg ggaccacggt ccgcctggag
     3481 ctgccctgca ccgagacctc accctccccc gccggtctca cccgcccggt ggaggcgggc
     3541 gtcgccgccc acgagtcggt gctcctcgtc gaggacgaac ccgccgtgcg ccgggccgcc
     3601 cagcggctgc tcgagcgggc cggccacgcg gtggtcgccg ccgccgacgg gcacgaagcc
     3661 gtcgatgccg tgctcgacgg gctgcgcccc acggtcctgc tcaccgacgt cgtcctgcct
     3721 ggcacgctca ccgggcacga cgtcgccgag cgggtccggg ccgtgatccc cgacgtgcgg
     3781 atcgtctacg cctcgggcta ctcccgcgac gtcctcaccg cggagcagct cgagtccgag
     3841 cacgcccggt tcgtctccaa gccgttcacc agtcgatcgc tgctcgacgc ggtcgccggc
     3901 gccgtcgagg tcgtggcatg accgggctgc tcgtcgtcga ggacgagccg accctggccc
     3961 gggcgctcgg gcgcgtgttc gccgaccact tcgaccacgt gcacgtcgcc gggacggcgc
     4021 gggaggcgag agccgtgctc gcccagcacc gacacgtcat cgacgtcgtg ttcaccgacg
     4081 tgatgctgcc cgacggcgac ggcatcaccc tggtggccga gctccgccag ctcgatccga
     4141 ccctcggcgc ggtcgtcgcg accggggtgg acgatccgct gaccgccgag cgagccgtcg
     4201 agctcggggt ccagggctac ctgctgaagc ccttcgagat gaccgaggcc cggatcaacg
     4261 ccgccaacgc ccagcactgg cgccggctcg agcaggagaa ccgggaccac cgcgagcgcc
     4321 tctccagcct ggtcgacgtc cggacccggg cgctggagcg gagccggtcc gagaccatca
     4381 cccggctgtc cgtggccgcc gagcaccgag acatggagac gagcagccac ctggagcgga
     4441 tgagcgccta cgcggcgatc ctcgcccggc gcgccggtct ggacccggag cactgcgagc
     4501 agatccgact cgccagcccg atgcacgaca tcggcaagct gggcatcccc gaccacgtcc
     4561 tgctccacga cgggccgttc accccggagc agcgccgcat catggggacc cacaccgtgc
     4621 tcggccaccg gatactcgct ggctccgagt caccgctgct cgagctgggg gcgaccatcg
     4681 cgctgacgca ccacgagtgg tgggacggca gcggctaccc gtgcgggctg gccggcaccg
     4741 acatccccct cgaggggcgc atcgtcgcca tcgccgacgt gttcgatgcg ctcgcctcgc
     4801 cccgtcgcta caagcgggcg ttcggcgtgg acgaggcggt cgagatcatg cggtccgagc
     4861 gtggcacgca cttcgatccg tggctcctcg accgcttcct cgccgacctc gacgagctgc
     4921 tgttcatccg atccgcccac cccgaccgtg ccgacgtggt gtacgccggc tgacggtcgg
     4981 ggcagagcag gtcggcgttc agaccgtgcc ggtgccggcg ctggccgtcg agtcggcctg
     5041 gaacttcagg tagccgccgt aggtcatgcc ggcggcgagc agcaggccga gccaggcacc
     5101 gatgcccagg taatcggtct cgaggatcag cttcagcacc agcaggccga gggtggcggc
     5161 agcgccgaag aagatggccc ggtcccacgt caccggcagg tcggggagct tggtctcggg
     5221 cttgaagacc cgcagcaacg tcacggccac gacgacgatc gtgaggagga gcgcgaggac
     5281 gccgaggaag gcgttctcct cctcgatcgc ggtcagcgat gcgctgaacg gaccgacgtc
     5341 gatgctgtgc cagggcagga acagcaggtc gatgatcagc acgatgcccg tgccggcgat
     5401 gatcttgtcg gacagggaaa gcttgtccag gtccatgatg agggcccacc tctcgtttgc
     5461 gtcgtgcacc gggtccggtg caccaccgaa acggtacgac gtcacgacgg agcgtgcggg
     5521 gattcccctc agccgaggag ggcgggcagg acctcggaga tcgagccctc gatgcgcacg
     5581 tcggcgaggt cgtccatctc ggtggggccg ccgttgacga tcagcacgga cgcgccgtgc
     5641 accttggcca gcgggaccac gttggcgatc gggtagacgc cgagggtgga gcccacggcg
     5701 agcagcaggt cggcccg
//
LOCUS       DATISU010000378         3040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_682848, whole genome shotgun sequence.
ACCESSION   DATISU010000378 DATISU010000000
VERSION     DATISU010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682848"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VLR27_14940"
     CDS             <1..649
                     /locus_tag="VLR27_14940"
                     /inference="COORDINATES: protein motif:HMM:NF013934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HSP04804.1"
                     /translation="PAPHNRIPNFVGAEAAAERLRSQPAWASAATVKSNPDSPQWPVR
                     QRALEDGKVVYMAVPRLAEDDPFFLLDPHDLADTPRKASSIKGATRSARTVTVAELEP
                     VDLVVTGCVAVDRSGARLGKGGGFSDLEYALAWEAGLIGPDTVVVTTVHDVQVLAEGD
                     IPMTDHDFRLDLVVTPDEVIECSPGDRGPAAIRWDELTEDKIRSIPLLARLAAEQ"
     gene            704..1294
                     /locus_tag="VLR27_14945"
     CDS             704..1294
                     /locus_tag="VLR27_14945"
                     /inference="COORDINATES: protein motif:HMM:NF016109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop repeat-containing protein"
                     /protein_id="HSP04805.1"
                     /translation="MLEQAVTAFAVVLALTQPVPQIIRVLRTRSVAGVSGATTWLGFT
                     INTAWVAYGVARDLLPVALLSTAYVVGYLVVGVLLVRGGNPKGIGPGALVGAGLAALA
                     AVGGWVALGTVLALAVAVQFLPQVVEAWTSDDLTGLAPGTYVVSGLDGIVWGGFGLMV
                     SDGPLVLYGVIMCTVAVAVLVPRARWAARQRALATA"
     gene            complement(1335..2441)
                     /locus_tag="VLR27_14950"
     CDS             complement(1335..2441)
                     /locus_tag="VLR27_14950"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP04806.1"
                     /translation="MTITDVPTTATAEPTVDDFVERLVGAVLGAQEVQGVYFGHRLGW
                     YDALADHGPMTSTQLAAATGSNERYAREWLEHQAVCGYLTVDDDTAAPQDRRFSLPAA
                     HAEVLTDQDSLAFVAPVAHLVAAVGHHLDAIADTFRTGGGVSWAQLGDEVREAQAAAN
                     RPLFLHQLGSELLPQAGDVDERLRNGARVADIGTGFGWSAIGLARAYPTITVDGFDTD
                     VPSIEAARRNAAAAGVADRVRFHVADGGDLATPTKPYDAVFAFECIHDLPDPVSVLAT
                     MRRLAGDDGAVVVMDENVAEAFHAPGDEVERLMYGYSLTCCLTDGMSHEHSAGTGTVM
                     RASTFTGYARQAGFADVHVLPIEHDFFRFYRLER"
     gene            2571..>3040
                     /locus_tag="VLR27_14955"
     CDS             2571..>3040
                     /locus_tag="VLR27_14955"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HSP04807.1"
                     /translation="MRARVQVLGAVAVTVGDETTTISSPSQRVVLSVLAARADRDVPT
                     DLLVEALWPDAPPRTAVSSLRTYVSRLRGLLGDALVGTPGGYRLAIDEVDLDLLRFER
                     LLDDAATAPPARALDLIDEALAMWRGPPFGDVGDVESIRPVAVRLEERWVTARE"
BASE COUNT          386 a         1106 c         1120 g          428 t
ORIGIN      
        1 cccggcaccg cacaaccgca tcccgaactt cgtcggggcc gaggccgccg ccgagcgcct
       61 ccgctcgcag ccggcctggg catcggccgc gacggtgaag tcgaacccgg actctcccca
      121 gtggccggtg cgccagcggg ccctggagga cggcaaggtc gtctacatgg ccgtcccccg
      181 cctcgccgag gacgatccct tcttcctgct cgacccccac gacctggccg acacgccccg
      241 gaaggcgtcg agcatcaagg gtgcgacccg cagcgcccgc acggtcaccg tggccgagct
      301 cgagcccgtc gacctcgtgg tcaccggctg cgtcgccgtc gaccgctccg gtgctcgcct
      361 cggcaagggc ggtggcttct ccgatctgga gtacgccctc gcctgggaag cgggcctcat
      421 cgggccggac acggtcgtgg tgaccaccgt gcacgacgtg caggtgctgg ccgagggcga
      481 catcccgatg accgaccacg acttccggct cgacctggtc gtcacccccg acgaggtgat
      541 cgagtgctcc ccgggcgacc ggggccccgc cgcgatccgc tgggacgagc tgaccgagga
      601 caagatccga tcgatcccgc tcctcgcccg cctcgccgcc gagcagtagt cgccgctaca
      661 tccgcactgc cagaacggtt cggggtgctg cgagactgtc ccgatgctcg agcaggcggt
      721 caccgccttc gccgtggtgc tggccctgac ccagccggtg ccccagatca tccgggtgct
      781 gcgcacgcgg tcggtcgccg gcgtctcggg tgccaccacg tggctcgggt tcaccatcaa
      841 cacggcgtgg gtcgcctacg gcgtcgcccg cgacctgctg ccggtcgccc tgctgtccac
      901 cgcgtacgtc gtcggctacc tcgtggtcgg cgtgctgctc gtgcggggtg gcaaccccaa
      961 gggcatcggc cccggcgccc tcgtgggtgc cggcctcgcc gcgctcgccg cggtcggggg
     1021 atgggtcgct ctcggcaccg tgctcgccct ggccgtcgcc gtgcagttcc tgccccaggt
     1081 ggtcgaggcg tggaccagcg acgacctcac cggcctcgcc cccgggacct acgtggtcag
     1141 cggcctcgac ggcatcgtgt ggggcgggtt cggcctgatg gtcagcgacg gacccctcgt
     1201 cctctacggc gtgatcatgt gcacggtggc ggtcgccgtc ctcgtgcccc gggcccgctg
     1261 ggccgcccgc cagcgggccc tcgccacggc ctgagcaccg gccgggacga gggcaccgcc
     1321 gtggcggcga gccgtcagcg ctccaaccgg tagaagcgga agaagtcgtg ctcgatcggc
     1381 aggacgtgca cgtcagcgaa gccggcctgg cgggcgtagc cggtgaaggt ggacgcccgc
     1441 atcacggtcc cggtcccggc cgagtgctcg tgcgacatgc cgtcggtgag gcagcacgtc
     1501 agcgagtagc cgtacatgag ccgctcgacc tcgtcgccag gtgcgtggaa cgcctcggcg
     1561 acgttctcgt ccatgacgac gacggcaccg tcgtcgccgg cgagccggcg catcgtggcc
     1621 agcaccgaca ccgggtcggg caggtcgtgg atgcactcga acgcgaacac ggcgtcgtag
     1681 ggcttcgtcg gggtggccag gtcaccaccg tcggcgacgt ggaagcggac ccgatcggcg
     1741 acgccggccg cggccgcgtt ccggcgtgcc gcctcgatcg acgggacgtc ggtgtcgaag
     1801 ccgtcgacgg tgatggtcgg gtaggcccgg gccagcccga tggccgacca gccgaacccg
     1861 gtgccgatgt cggcgacccg tgcgccgttg cgcaggcgct cgtcgacgtc acccgcctgg
     1921 gggagcagct cggagccgag ctggtgcagg aagagcggtc ggttcgccgc agcctgcgcc
     1981 tcccggacct cgtcacccag ctgggcccag ctgacgccgc caccggtgcg gaacgtgtcc
     2041 gcgatggcgt cgaggtggtg gccgacggcg gcgacgaggt gggccacggg ggcgacgaag
     2101 gcgaggctgt cctggtcggt gagcacctcg gcgtgggccg ccggcaggga gaaccggcga
     2161 tcctggggtg cggccgtgtc gtcgtcgacc gtcaggtagc cgcacacggc ctggtgctcg
     2221 agccactccc gggcgtagcg ctcgttgctc ccggtggctg cggccaactg cgtcgaggtc
     2281 atgggcccgt ggtcggccag tgcgtcgtac cagccgagcc ggtggccgaa gtacacgccc
     2341 tggacctcct gggcgccgag cacggcgccg acgagccgct cgacgaagtc gtcgacggtc
     2401 ggctcggcgg tggcggtggt ggggacgtcg gtgatggtca tggtggtgct cctgtcggtg
     2461 gtggtcgacg gccagcatcc gacgggggcg ttgcagcggc gtcgcagcgg gatcgcagcc
     2521 cgggagacaa agccatcgac ccccgagccc ctggtccgta cgctcgccac gtgcgagcgc
     2581 gggtgcaggt cctcggagcg gtcgccgtga ccgtcggcga cgagaccacg acgatctcga
     2641 gccccagcca acgggtcgtc ctgtcggtgc tcgccgcccg cgccgaccgg gacgtcccca
     2701 ccgacctcct cgtcgaggcg ctctggcccg acgcgccgcc ccgcacggcg gtcagctcgc
     2761 tgcgcaccta cgtgtcgagg ctgcgcggcc tgctcggtga cgccctggtc gggacgcccg
     2821 gcggctaccg gctggccatc gacgaggtcg acctcgacct gctgcgcttc gaacgactcc
     2881 tcgacgacgc cgcgacggca ccgccggccc gggcgctcga cctgatcgac gaggcgctgg
     2941 ccatgtggcg gggacccccg ttcggcgacg tcggggacgt cgagtcgatc cggccggtcg
     3001 cggtgcggct ggaggagcgg tgggtgacgg cacgggagga
//
LOCUS       DATISU010000379        10074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_123748, whole genome shotgun sequence.
ACCESSION   DATISU010000379 DATISU010000000
VERSION     DATISU010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10074
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123748"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VLR27_14960"
     CDS             complement(<1..471)
                     /locus_tag="VLR27_14960"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HSP04808.1"
                     /translation="MASPRPLIGLTTYPPNPGDRYELPRPYVDAVRRAGGEPVLVPPG
                     VEDAAGLLDRLDGLLLAGGGDIDPARYGGPSHETVYALDPDRDESELALAHLVLERGT
                     PTLAICRGSQVVNVALGGSLHVHLPDVVDGTVVHRKEPEALRGMPGPTPHEIEVE"
     gene            complement(510..1331)
                     /locus_tag="VLR27_14965"
     CDS             complement(510..1331)
                     /locus_tag="VLR27_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04809.1"
                     /translation="MRIRTLLAAAASAACLSAVVALGAPTASAAPSWAPASSAAVHPG
                     VQTYTAGGQCTANFVFTDATDVYIGQAAHCSGTGGQTETNGCDAGSLPLGTPVEVDGA
                     TQPGTMVYNSWLTMQDVGETDPNACAYNDFALIRLDPADHGLVNPSIPFWGGPVAIGG
                     ATSTLERVYSYGNSSLRLGIEALSPKTGTSLGSNGGGWNHPVYTVTPGIPGDSGSAFL
                     DGDGNAIGVLSTLALAPLAGSNGVSDIGLALAYMEANTSLDVDLAPGTEPFSPIL"
     gene            complement(1416..2753)
                     /locus_tag="VLR27_14970"
     CDS             complement(1416..2753)
                     /locus_tag="VLR27_14970"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HSP04810.1"
                     /translation="MTPGLLSVDDLRAAVAAGTIDTVICAFPDLYGRLMGKRLDARFF
                     LEDPAHGTHACDYLFTVDMEMEPIEGYGFASWAKGYGDVHLVPDLATLRRCSWLDRTA
                     LVMCDAALGHDPVRVAPRSVLAAQVARAGEHGYTAKAASELEYFLYRDSFADAATRDH
                     RDLHPAGWYIEDYHLLQASRTEDLNGPFRRHLTASGIPVESTKGEWGRGQHELNIAYC
                     DVANMADRHVLLKHACKEIADQLGASVTFMAKPDSAEAGSSSHLHLSLWRGNVNVFAS
                     DETFRHFLGGWLAHAAELMVCFAPTINSYKRYQDQSWAPTALAWSPDNRTAGFRIVGE
                     GPAKRIECRIPGADVNPYLAYAAALASGIDGIEQGTEPPEALDGDAYATDDLPPLPRT
                     LVEAVAAFEGSDFARRAFGEDVVDHYVHFFRTEVAQYDAAVTDWERRRYFERI"
     gene            complement(2810..3598)
                     /locus_tag="VLR27_14975"
     CDS             complement(2810..3598)
                     /locus_tag="VLR27_14975"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HSP04811.1"
                     /translation="MSDDLFPDVRRHVLGRLGLSFDEDRSAVRLPDEPVLLDPDGHVN
                     FGVLGVLLDMGSSGALAGEEFSPWVHADIAVHRLRAPQGALSTTARTLRRGKRTGVVE
                     LDVHDASGALVAASTQQITFLGPPPEPASDMSERHGAFQSMFDGRCHLTEPLPEYLGI
                     RDDGDGTWSMDHGPDRTNGMSGLHGGVATTFVDAAAAGAVRRARGGPARTVSAAVRYL
                     RPALHGPFSVTPEVLHDDGRVAVVRTPVLDVEGTLVISADVHVG"
     gene            complement(3736..4005)
                     /locus_tag="VLR27_14980"
     CDS             complement(3736..4005)
                     /locus_tag="VLR27_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04812.1"
                     /translation="MQFQALFSSRDADSVSVDGILHDGTGPGRAATITGARPTSGVLA
                     VVIPGTLDAWAEGMSMLDVTEGQGWLEMTDGATRLSLRPVDAHHA"
     gene            complement(4167..4331)
                     /gene="rpmG"
                     /locus_tag="VLR27_14985"
     CDS             complement(4167..4331)
                     /gene="rpmG"
                     /locus_tag="VLR27_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HSP04813.1"
                     /translation="MPKNDKRPIIKLKSTGGTGYTYVTRKNKTNTRERLELSKYDPVL
                     RKHVTFKEER"
     gene            complement(4331..4567)
                     /gene="rpmB"
                     /locus_tag="VLR27_14990"
     CDS             complement(4331..4567)
                     /gene="rpmB"
                     /locus_tag="VLR27_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HSP04814.1"
                     /translation="MSKICQVTGRKPSFGKTISHSHRRGNRRWTPNIQKQRFWVESEK
                     RWVTLTVSAKGIKTINKKGIDAVLADMRRKGVKV"
     gene            complement(4746..6068)
                     /locus_tag="VLR27_14995"
     CDS             complement(4746..6068)
                     /locus_tag="VLR27_14995"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HSP04815.1"
                     /translation="MHDHSHDLGTAPEVARALRLAVLAIVVLTAIGLFVWRPDGGVAI
                     PSAVDGQGERVDATVTGTSEIECAGTDFGGVPIVCEEIELDITSGANAGRTGSFELYP
                     QPGSPSFDAGDRIVVNEYAVPEGGISYTFADYQRSRPMLLLLGIFVVAVIAMGRFQGL
                     RALAGLVVSGIVLLAYAFPALLDGKPPLGIALVSSVIIAVAALYLSHGINDTTTVALL
                     GTLAALGLTGLLAAVFTAAAQITGLASEDQIFLMVSAEGVDVRGLVLAGIVLGSLGVL
                     DDVTVTQVSAVARLREANPGYGIAELYRSGVRIGRDHIASVVNTLVLAYAGAALPLLL
                     FYSNIGIGLSESVTSEIVAVEIIRTLVGSIGLVAAVPLTTGLAAVVVTGTGFRRRDPH
                     PDHDDEPAVAPATPATPAAPIARPPTAEADRPEPEVDWDEFGPRPEDF"
     gene            6132..6872
                     /locus_tag="VLR27_15000"
     CDS             6132..6872
                     /locus_tag="VLR27_15000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HSP04816.1"
                     /translation="MEPCTHLAHLRADIDRILATPAEHLDALVKACPGWTVADLLGHH
                     AGVFRFATAQLRAEPGSDFVPFDPPDEGVPPLEMLAVAGQEVLAALADTDPTEHRPNW
                     ADAPTSAFWFRRLAHETAIHRVDAQLAHTTPDPIDAALAVDGVDELGEVFLRHAGRRG
                     ITGTGETVHLHATDDEVTSGEISGGEWMFTFHEDGVDVTHDHGKGDMAVRGPAGQLVL
                     FAWNRRPVEVECFGDPDPLAFWARTVRI"
     gene            6885..7676
                     /locus_tag="VLR27_15005"
     CDS             6885..7676
                     /locus_tag="VLR27_15005"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSP04817.1"
                     /translation="MTAPPVDPELLTWAVELTKEAGRRTLTRFRSDDLAIEAKGDGTP
                     VTEADKDAEAYIRAELTAAHPDDAVLGEEEGDQEGTTGRRWIIDPIDGTKAFMRGVPL
                     YSNLLAVEDDHGGAIGVINLPAAGECVYAGRGLGAFQDDRQGERRPARVGPRSTTQPF
                     LTASGFTRWDDDALLRVKHAGWGLRTWGDGFGYAMVATGRIDAMVDPSAALWDIAPMP
                     VILSEAGGRFSAWSGSPDPREGSGVATNGEGHDDLLALLRGDTQP"
     gene            complement(7738..8733)
                     /locus_tag="VLR27_15010"
     CDS             complement(7738..8733)
                     /locus_tag="VLR27_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04818.1"
                     /translation="MSPAPDRNRTAGVLGVGATGARAARQLASADGIDSVVLGAHGER
                     PIALQQSLGSAARIGEPIAADVGVVVLAGPAGTHVEAARRAIDLGVDVVSTSDAIPDV
                     EGLLALDGRARAAGARVVVGAGFSPGLSCVLARHLADWVDEVDEVHLSRFGTGGPACA
                     RQHHRALAARAIDWRDGEWVRRRGGSGRELSVFPDPVGSLDCYRAALPDALLLRPAFP
                     GASRIAARQAANRRQRLLALLPMLRRPHPEGRIGAIRVEVRGRRDGEVVCEVVGAIDR
                     PAVAAGAVAGLAARWCLDGRVEPGAAGLAPRVEPVPFLQELADFGVRAAVFVGVA"
     gene            complement(8730..8867)
                     /locus_tag="VLR27_15015"
     CDS             complement(8730..8867)
                     /locus_tag="VLR27_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04819.1"
                     /translation="MTLLRRAIAALGVAAFIAAGLRIRGTGGTPPQTGGWRELRGPDL
                     T"
     gene            complement(8951..9024)
                     /locus_tag="VLR27_15020"
     tRNA            complement(8951..9024)
                     /locus_tag="VLR27_15020"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8988..8990),aa:Ile,seq:gat)
     gene            complement(9091..9696)
                     /locus_tag="VLR27_15025"
     CDS             complement(9091..9696)
                     /locus_tag="VLR27_15025"
                     /inference="COORDINATES: protein motif:HMM:NF023511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HSP04820.1"
                     /translation="MSTDPTGVRPSPDATGPQPTVPDERRGTQAPAPSRSRRGRGRKV
                     RKVHRVVRHLDIWSVAKLAAVFALCWYVVGMISGYLLWQAADRVGTIDGIEGFFEDAG
                     GYDTFEILGDIVFRAAAVVGLLLAALGVFAAVLGALLFNLISDLTGGVRMTVIDEDLI
                     VAPARRRTVAPGGPPQTPNGQDSQSRPDRTAERPATSSVER"
     gene            complement(9883..>10074)
                     /locus_tag="VLR27_15030"
     CDS             complement(9883..>10074)
                     /locus_tag="VLR27_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HSP04821.1"
                     /translation="TSRGNEVLGSVTTRTPGGTKVTALAATGTSTTVALAALGAVLLL
                     AGFGLQVTARRVQSSTVGA"
BASE COUNT         1397 a         3450 c         3910 g         1317 t
ORIGIN      
        1 ctccacctcg atctcgtgcg gcgtcggacc gggcatgccg cgcagggcct cgggctcctt
       61 gcggtggacg acggtgccgt cgaccacgtc gggcaggtgg acatggaggc tgccgccgag
      121 ggcgacgttc acgacctggg agccacggca gatggccagc gtcggcgtcc cccgctcgag
      181 gacgaggtgg gccagggcca gctcgctctc gtcccggtcg gggtcgaggg cgtacacggt
      241 ctcgtgcgac ggaccgccgt agcgggccgg gtcgatgtcg ccgccgccgg cgaggagcag
      301 cccgtcgagc cggtcgagca acccggcagc gtcctcgacg cccggcggga ccagcaccgg
      361 ctcgcccccg gcccgacgga cggcgtcgac gtagggccgg gggagctcgt agcggtcgcc
      421 ggggttcggc gggtacgtgg tgagcccgat gagcgggcga gggctggcca ttggcagcga
      481 acgctaccga ccgcccccgc ccggtcgagc tagaggatcg gcgagaaggg ctccgtgccc
      541 ggggcgagat cgacgtcgag ggaggtgttg gcctccatgt aggcgagggc gaggccgatg
      601 tcgctcacgc cgttcgagcc ggccagcggg gccagggcga gcgtggacag cacgccgatg
      661 gcgttgccgt cgccgtcgag gaacgccgag cccgagtcgc ccgggatgcc cggggtcacg
      721 gtgtacaccg ggtggttcca gccgccgccg ttcgagccga ggctggtgcc ggtcttgggc
      781 gacagcgcct cgatgcccag gcgcaggctc gagttgccgt agctgtacac ccgctcgagg
      841 gtgctggtgg cgccgccgat ggcgacgggg ccgccccaga acgggatcga ggggttgacc
      901 aggccgtggt cggccgggtc gaggcggatg agcgcgaagt cgttgtaggc gcacgcgttc
      961 gggtcggtct cgccgacgtc ctgcatcgtc agccacgagt tgtagaccat cgtgcccggc
     1021 tgggtggcgc cgtcgacctc gacgggcgtg ccgaggggca gcgagccggc gtcgcagccg
     1081 ttggtctcgg tctggccgcc ggtgccgctg cagtgggcgg cctggccgat gtagacgtcg
     1141 gtggcgtcgg tgaagacgaa gttggcggtg cactggccgc cggcggtgta ggtctgcacg
     1201 ccggggtgca cggcggcgct gcttgccggc gcccacgacg gggcggcgga ggcggtgggt
     1261 gcgccgaggg cgacgacggc ggagaggcag gccgcggagg cggcagcagc caggagggta
     1321 cggatgcgca cgagggtggt ccttgtctgt cgggttccgg gaggaacggg gcgcccgccc
     1381 cgcacgggaa cggtcccgac ccgaggacct tgcggtcaga tccgttcgaa gtaccgccgg
     1441 cgctcccagt cggtgaccgc ggcgtcgtac tgggccacct cggtgcggaa gaagtggacg
     1501 tagtggtcca ccacgtcctc cccgaacgcc cgccgggcga agtcggaccc ctcgaacgcg
     1561 gccacggctt cgacgagcgt gcggggcagg ggcggcaggt cgtcggtggc gtacgcgtcg
     1621 ccgtcgagcg cctcgggcgg ttcggtgccc tgctcgatgc cgtcgatccc gctggcgagg
     1681 gcagcggcgt aggcgaggta ggggttcacg tccgccccgg ggatgcggca ctcgatgcgc
     1741 ttggccggtc cctcccccac gatccggaac ccggcggtgc ggttgtcggg ggaccaggcg
     1801 agcgcggtcg gcgcccacga ctggtcctgg tagcgcttgt agctgttgat cgtgggggcg
     1861 aagcacacca tcagctccgc agcgtgggcg agccacccgc cgaggaagtg ccggaaggtc
     1921 tcgtcgctgg cgaacacgtt cacgttgccc cgccacagcg agaggtggag gtgcgacgac
     1981 gaaccggcct cggcgctgtc tggcttggcc atgaacgtga ccgacgcgcc gagctggtcg
     2041 gcgatctcct tgcacgcgtg cttcagcagg acgtggcggt cggccatgtt cgcgacgtcg
     2101 cagtaggcga tgttcagctc gtgctggccc cggccccact cccccttggt cgactccacc
     2161 gggatgcccg acgcggtcag gtgacgccgg aaggggccgt tgaggtcctc ggtccggctg
     2221 gcctggagca ggtggtagtc ctcgatgtac cagccggccg ggtgcaggtc gcggtggtcc
     2281 cgggtggcgg catcggcgaa ggagtcccgg tacaggaagt actcgagctc gctggccgcc
     2341 ttggcggtgt agccgtgctc accggcccgg gcgacctggg cggcgagcac gctgcggggg
     2401 gcgacccgga ccggatcgtg gccgagcgcc gcgtcgcaca tgacgagggc ggtgcggtcg
     2461 agccaggagc agcggcgcag ggtggcgagg tcggggacga ggtgcacgtc gccgtagccc
     2521 ttggcccagc tggcgaaccc gtagccctcg atcggctcca tctccatgtc gacggtgaag
     2581 aggtagtcgc aggcgtgggt gccgtgcgcg gggtcctcga ggaagaagcg ggcgtcgagg
     2641 cgcttgccca tgagccgccc gtacaggtcg gggaaggcgc agatgacggt gtcgatcgtc
     2701 ccggccgcca ccgcggctcg caggtcgtcg acggacagga ggccgggcgt cacgccggca
     2761 gtcgaccacg ggaccccggc cggggcaagg gtctggcctg tccccgacct cagccgacgt
     2821 ggacgtcggc gctgatgacg agggtcccct cgacgtccag cacgggcgtg cgcaccacgg
     2881 ccacgcggcc gtcatcgtgc aggacctcgg gcgtcaccga gaacgggccg tggagcgcgg
     2941 gccgcaggta gcggaccgcg gcgctgacgg tgcgagccgg cccaccgcgg gcccggcgga
     3001 cggcgccggc ggcggcggca tcgacgaagg tcgtggccac gccgccgtgc aggccgctca
     3061 tgccgttggt gcgatccggt ccgtggtcca tggaccaagt gccgtcgccg tcgtcccgga
     3121 tgcccaggta ctccggcagc ggctcggtga ggtggcagcg accgtcgaac atcgactgga
     3181 acgcgccgtg gcgctcgctc atgtccgagg ccggctcggg cggcggcccg aggaaggtga
     3241 tctgctgggt gctggcggcg acgagcgcgc ccgaggcgtc gtgcacgtcg agctcgacca
     3301 cgccggtgcg cttgccccga cgcagggtgc gggcggtggt ggacagcgcg ccctgcggtg
     3361 cccgcagccg gtgcacggcg atgtcggcat ggacccacgg gctgaactcc tcgccggcga
     3421 gggcacccga ggagcccatg tccagcagga ccccgaggac gccgaagttc acgtggccgt
     3481 ccgggtcgag caggaccggt tcgtcgggca gccgcaccgc gctgcggtcc tcgtcgaagg
     3541 agagcccgag ccggccgagc acgtgccggc ggacgtccgg gaacaggtcg tcgctcaccc
     3601 tgccagtctc tcttgaccgg gcggtcaagt tccaatcggt ggggtcgctg cggcggtcag
     3661 cacccggggt gggttcggcc ggcgggcccg ccggagaccc cacctcccca tgcggacccg
     3721 ccggtcgaga gctgctcagg cgtggtgggc gtccacgggc cggaggctga gtctggtggc
     3781 accgtcggtc atctcgagcc agccctggcc ctcggtgacg tcgagcatcg acatcccctc
     3841 ggcccaggcg tcgagggtgc cggggatgac cacggccagc acgccgctgg tggggcgggc
     3901 gccggtgatg gtggcggccc ggccggggcc ggtgccgtcg tggaggatcc cgtcgacgct
     3961 gaccgagtcg gcgtcacggc tggagaagag cgcttggaac tgcacgggac gacctcgggg
     4021 tgaaggggcg accggggggt ggccggttcc tacccctgca cccctccggt cattcggcgg
     4081 ctgcgcccgg gcgcggcgag ggccggtgcc cagcggcacc ggccctcgtc tcgtgctcgt
     4141 cgggcccggg gaccgggccg gcgtgcttag cgctcttcct tgaaggtgac gtgcttgcga
     4201 aggacggggt cgtacttcga cagctcgagg cgctcgcggg tgttggtctt gttcttgcgg
     4261 gtcacgtacg tgtacccggt gccaccggtg ctcttgagct tgatgatcgg gcgcttgtcg
     4321 ttcttgggca tcagaccttc actcccttgc ggcgcatgtc ggccagcacg gcgtcgatgc
     4381 ccttcttgtt gatcgtcttg atccccttgg ccgagaccgt gagggtgacc cagcgcttct
     4441 ccgactcgac ccagaagcgc tgcttctgga tgttcggggt ccagcggcgg ttgccacggc
     4501 ggtgggagtg ggagatggtc ttgccgaacg acggcttgcg tccggtgacc tggcagatct
     4561 tcgacatggt gcttccttcg aggggcgcag caccgcctca cgggcgtgag cgcgggcgcc
     4621 ggtacgagcg acggaatagt ttcgcaggcg cgccaggaga acgacaaccc ggcgcccgac
     4681 cggttcggcc tgcggggaaa ccacatccat gtaatcacag gtgtggaaga tgctgtggat
     4741 aacccctaga agtcctcggg acggggtccg aactcgtccc agtccacctc cggctccggc
     4801 cgatctgcct ccgcagtcgg cgggcgggcg atcggcgctg cgggcgtcgc cggggtcgcc
     4861 ggcgccacgg cgggctcgtc gtcgtggtcg gggtgcgggt cgcgccggcg gaacccggtg
     4921 ccggtcacga ccacggcggc caggccggtc gtgagcggga cggcggcgac caggccgatg
     4981 ctgccgacga gggtgcggat gatctcgacg gcgacgatct cggaggtgac cgactcggag
     5041 agcccgatcc cgatgttgga gtagaagagc agcagcggca gggcggcgcc ggcgtaggcc
     5101 aggacgaggg tgttcacgac cgaggcgatg tggtcgcggc cgatgcgcac gccgctgcgg
     5161 tacagctcgg cgatgccgta gccggggttg gcctcgcgca ggcgggccac ggccgagacc
     5221 tgggtgacgg tgacgtcgtc gagcacgccg aggctgccga gcacgatgcc ggccagcacc
     5281 aggccccgca cgtcgacccc ctcggccgac accatgagga agatctggtc ctccgacgcc
     5341 agcccggtga tctgggcggc ggcggtgaac accgcggcga gcaggccggt gagcccgagc
     5401 gcggcgaggg tcccgaggag cgcgacggtg gtggtgtcgt tgatgccgtg ggacaggtac
     5461 agcgcggcca cggcgatgat caccgaggac acgagcgcga tgccgagcgg gggcttgccg
     5521 tcgagcaggg cggggaacgc gtaggcgagc aggacgatgc ccgacaccac gaggccggcg
     5581 agggcgcgca ggccctggaa gcgacccatg gcgatgaccg ccaccacgaa gatgccgagc
     5641 aggagcagca tcggccggga tcgctggtag tcggcgaagg tgtaggagat gccgccctcg
     5701 ggcaccgcgt actcgttcac gacgatgcgg tcgccagcgt cgaacgatgg gctgccgggc
     5761 tgggggtaga gctcgaagct gccggtccgg ccggcgttgg cgccgctggt gatgtcgagc
     5821 tcgatctcct cgcagacgat gggcaccccg ccgaagtcgg tgccggcgca ctcgatctcg
     5881 gacgtgcccg tgaccgtggc atcgacccgt tcgccctgcc cgtcgacggc ggaggggatg
     5941 gccaccccac cgtcgggtcg ccagacgaac agcccgatgg cggtgagcac gacgatggcc
     6001 agcacggcga ggcgcagggc gcgggccacc tcgggggcgg tgcccagatc gtgggagtga
     6061 tcgtgcacgc cagcaatggt cgcccaccgg accgactgcc gcgcggaccg tcgccggtac
     6121 ggtcgggacc gatggagccc tgcacccacc tcgcccacct gcgcgccgac atcgaccgga
     6181 tcctcgccac gcccgccgag cacctggacg ccctggtgaa ggcgtgcccg gggtggaccg
     6241 ttgccgacct gctggggcac cacgccggcg tgttccggtt cgccacggcg cagctgcggg
     6301 ccgagccggg atcggacttc gtccccttcg acccgcccga cgaaggcgtc ccgcccctcg
     6361 agatgctggc ggtcgccggc caggaggtcc tcgccgccct cgccgacacg gacccgaccg
     6421 agcaccggcc caactgggcg gacgccccca cctcggcgtt ctggttccgc cgcctcgccc
     6481 acgagaccgc gatccaccgg gtggatgccc agctcgccca cacgaccccg gaccccatcg
     6541 acgccgcact cgccgtcgac ggcgtcgacg agctgggcga ggtgttcctc cgccacgccg
     6601 gacgccgcgg catcaccggc acgggcgaga ccgtgcacct ccacgccacc gatgacgagg
     6661 tcacctccgg cgagatcagc ggcggcgagt ggatgttcac cttccacgag gacggcgtcg
     6721 acgtcaccca cgaccacggc aagggcgaca tggcggtgcg cggtccggcc ggacagctgg
     6781 tgctgttcgc ctggaaccgc cggcccgtcg aggtcgagtg cttcggcgat cccgacccgc
     6841 tggcgttctg ggcccggacg gtgcggatct agcaccctgg cgggatgacc gctccccccg
     6901 tcgaccccga gctgctgacc tgggccgtgg agctcacgaa ggaggcaggc cgccggacgc
     6961 tcacccgctt ccgcagcgac gacctggcca tcgaggccaa gggcgacggc accccggtga
     7021 ccgaggccga caaggacgcc gaggcctaca tccgggccga gctcaccgcc gcccaccccg
     7081 acgacgccgt gctcggcgag gaggaggggg accaggaggg gaccacgggc cgacgctgga
     7141 tcatcgatcc catcgacggc accaaggcct tcatgcgggg cgtgccgctg tactcgaacc
     7201 tgctcgccgt cgaggatgac cacggcgggg cgatcggtgt catcaacctg ccggccgccg
     7261 gcgagtgcgt ctacgccggc cgggggctgg gcgcgttcca ggacgaccgc cagggcgagc
     7321 gccggccggc ccgggtcggc ccccgctcga ccacgcagcc gttcctcacc gcctcgggct
     7381 tcacccgctg ggacgacgac gccctcctgc gggtcaagca cgccggctgg ggcctgcgca
     7441 cctggggtga cggcttcggc tacgccatgg tcgccaccgg tcgcatcgac gcgatggtcg
     7501 acccgtcggc cgcgctgtgg gacatcgccc ccatgccggt gatcctgtcc gaggccggcg
     7561 gccggttcag cgcctggtcc ggctcacccg acccgcgcga gggctcgggc gtggccacca
     7621 acggcgaggg ccacgacgac ctgctcgccc tcctccgagg cgacacccag ccctgacccg
     7681 ttctggccta catccatgac cgtctgacgg cacggatgta ggccagaacg agtggggtca
     7741 ggcgacgccg acgaagaccg cagcccggac gccgaagtcg gcgagctcct ggaggaacgg
     7801 caccggctcg acccggggtg cgaggccggc ggcgcccggt tcgacgcgac cgtcgaggca
     7861 ccagcgggcg gcgaggccgg ccacggcgcc ggcggccacg gcgggacgat cgatggcgcc
     7921 cacgacctcg cagacgacct cgccgtcgcg tcggccccgg acctcgacgc ggatggcgcc
     7981 gatgcggccc tcggggtgcg gccggcgcag catcggcagc agggccagga ggcgctggcg
     8041 acggttggcg gcctggcgcg cggcgatccg ggatgcaccc gggaacgccg ggcgcaggag
     8101 cagggcgtcg ggcagggcgg cccggtagca gtcgagcgaa ccgacggggt cggggaacac
     8161 gctgagctcg cgtcccgagc caccgcggcg acggacccac tcgccgtcgc gccagtcgat
     8221 ggcccgggcg gcgagggcgc ggtggtgctg gcgcgcgcag gccggcccgc ccgtcccgaa
     8281 gcgggagagg tggacctcgt cgacctcgtc gacccagtcg gcgaggtggc gggccagcac
     8341 gcagctgagc ccgggcgaga acccggcgcc gacgaccacc cgcgcaccgg cggcgcgggc
     8401 tcgaccgtcg agcgcgagga ggccctcgac gtcggggatg gcgtcgctgg tggagaccac
     8461 gtcgaccccg aggtcgatgg cccgccgggc cgcttccacg tgcgtgccgg cggggccggc
     8521 caggaccacg accccgacgt cagcggcgat cggctcaccg atccgggcgg ccgatccgag
     8581 ggattgctgg agtgcgatcg gacgctcgcc gtgggcgccg agcacgaccg agtcgatccc
     8641 gtcggccgac gcgagctggc gagcggctcg tgcgccggtg gcgccgaccc cgaggacccc
     8701 ggcggtgcgg ttccggtccg gtgccgggct cacgtgaggt cgggaccgcg cagctcgcgc
     8761 cagcccccgg tctgcggggg tgttccgccg gtgccacgga tgcgcaggcc agcggcgatg
     8821 aaggcggcga cgcccagggc ggcgatggct cggcgcagca gcgtcacgtc ggcggtgctc
     8881 cttcggcgct ccggtagcgg ggagttgcga ggcgcagccg aacaactccg aggaacccga
     8941 agggttcccg tggggctaac aagaatcgaa cttgtgacct cacccttatc aggggtgcgc
     9001 tctaaccgac tgagctatag ccccggagga ggggacaaca tagcagcagg acccgggggc
     9061 ccgaccaagc ggacgggccg gctcgggggc tcagcgctcg acgctggagg tcgccggacg
     9121 ctcggctgtg cggtcggggc gggactggct gtcctgcccg ttgggggtct ggggcggacc
     9181 gccgggcgca acggtgcggc gacgggccgg agcgacgatg aggtcctcgt cgatgacggt
     9241 catgcgaacg ccgccggtga ggtcgctgat gaggttgaac aggagggcgc cgagaaccgc
     9301 agcgaacacc ccgagcgccg ccaggagcag gccgacgacg gccgcggcgc ggaagacgat
     9361 gtcaccgagg atctcgaagg tgtcgtagcc gccggcatct tcgaagaagc cctcgatgcc
     9421 gtcaatggtg ccgacccggt ccgcggcctg ccacagcagg tagccgctga tcatgccgac
     9481 cacgtaccag cacagggcga agacggcggc cagcttggcc accgaccaga tgtcgaggtg
     9541 gcggacgacc cggtggacct tgcggacctt gcgtccccga ccgcggcggg agcgggacgg
     9601 cgccggcgcc tgggtgccgc ggcgctcgtc cggcacggtc ggctggggac ccgtggcatc
     9661 gggcgagggc cgcacacctg tggggtcggt cgacatcgtg ctccagtgta ggaatgggcg
     9721 tcccgcttgc ccgaccgcgg cgatcgtagc ggtggcgtcc ccgggcgcgg tgccgcccgg
     9781 tgagcgctcc cgcacgccca ccgggtcggc cgctgcgctg ccccgcccag caacgctgcc
     9841 cgctcgcatc cccggcccag ctccggggtc gcgttctgtg tgtcaggccc ccacggtgga
     9901 ggactggacc cggcgggcgg tgacctgcag accgaagccg gcgagcagga gcaccgcgcc
     9961 gagggcggcg agggcgacgg tcgtgctggt gcccgtggca gccagcgcgg tgaccttggt
    10021 gccgcccggc gtccgggtgg tgaccgaacc gaggacctcg ttgcccctcg acgt
//
LOCUS       DATISU010000380         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_171117, whole genome shotgun sequence.
ACCESSION   DATISU010000380 DATISU010000000
VERSION     DATISU010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171117"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VLR27_15035"
     CDS             complement(<1..680)
                     /locus_tag="VLR27_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSP04822.1"
                     /translation="MGILDRVLRTGEGKRLRALQGLVPEINALEPTYQAMSDDELKHQ
                     TVLFRERLEAGEDLDDIMLEAFAVCREAATRVLGQRHFDVQLMGGAALHFGWVAEMKT
                     GEGKTLTSTLPLYLNGLSGKGTHIITVNEYLATFQSEWMGRLFKWLGLSVGLIVPGNR
                     DADFKREQYAADITYGINNEFGFDYLRDNMAMSRERQVQRGFSYAIVDEVDSILIDEA
                     RTPLIISGR"
     gene            complement(735..1634)
                     /locus_tag="VLR27_15040"
     CDS             complement(735..1634)
                     /locus_tag="VLR27_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04823.1"
                     /translation="MRRSRSSLAFVLLVAVVGLVLGISPADAEGKGNGKGKGKGGTTS
                     TVQWAPADVAAITPGAEMLTNGAQCTANFIFTDGADVLIGYAAHCAGDGGAATDTDGC
                     LAPSLPTDGSTQVEIEGASQPGTLVYSSWETMQRVGETDPDTCLHNDFALVRIDPADH
                     GAVNPSIPYWGGPTALNTDGTGAGETVHSYGNSSLRLGIRALSPKTGLGLGTTAGGWT
                     HPVYTVSPGIPGDSGSALLDADGAAAGVLSTLALAPLAGSNNYSDLASALEYMHSNGG
                     PAAALALGTEAFDGAQLPLDLGL"
     gene            1783..2484
                     /locus_tag="VLR27_15045"
     CDS             1783..2484
                     /locus_tag="VLR27_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04824.1"
                     /translation="MLAVGFVAACSSGAEVAAPEAGVTAPTTTVRTVPPAVAGALRAE
                     VPPPAEPRRDDLEHEVLLYGDSVALLVADELAVELDAPLVVDAVDCRRLDAAFPGPCG
                     GVPAGTDVASGLDDLGPATERLDDPAAAIAVVVIANNAALAASDLDAAMAALGAVPRV
                     WWVTTRVDGRAWQDPNNQLIAELADRDPRAEVIDWFDASEDQGWLVDNVHPGDEGQAA
                     LADLIAAHVRCDCTP"
     gene            2481..>3911
                     /locus_tag="VLR27_15050"
     CDS             2481..>3911
                     /locus_tag="VLR27_15050"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HSP04825.1"
                     /translation="MTDLADPRTAPDDSRWPLRIEEPPPAYGPSLGRGVGYLPGIDGL
                     RALAVVAVLAYHLDASWATGGYLGVEVFFVVSGFLITSLLLAEHREHDRIDLLAFWRR
                     RARRLLPAVFVLVGLVFAYAAVVLPTGELHRFRGDGIASLLYVQNWHAIFTEQPYFEA
                     FGRPSPLRHLWSLAIEEQFYLLWPLLLPIGLRRLGRRRTVIAVAGAILVSVVLMAATA
                     DITAPERAYYGTDTRLFGILSGALLAFAWRPQRARGDVGPAARRVIEGAGIAALVLLA
                     WQHATRSEFDGWTYPWGFLLVDVLTLVTIIAVTHPASRLERSVGSGPLQALGRRSYSI
                     YLWHWPVIVFTRPGVDWGLTGPAALAARLALVAVLAEASYRLVERPVRDGTLQRWLAG
                     ATLPSPAALLRRRRAVGIAALAGLLLAVTVIAAPPGPGNEVAVGTVADQPSAITLPPV
                     PPTPSTAPPTTAAAAPADPPPTTAPTT"
BASE COUNT          453 a         1477 c         1418 g          563 t
ORIGIN      
        1 cggcccgaga tgatgagcgg ggtgcgggcc tcgtcgatga ggatcgagtc gacctcgtcg
       61 acgatcgcgt agctgaagcc ccgctggacc tggcgctcgc ggctcatggc catgttgtcg
      121 cgcaggtagt cgaagccgaa ctcgttgttg atgccgtagg tgatgtcggc ggcgtactgc
      181 tcgcgcttga agtcggcgtc gcggttgccc ggcacgatga ggcccacgga caggccgagc
      241 cacttgaaga gccggcccat ccactcggac tggaacgtgg ccaggtactc gttgaccgtg
      301 atgatgtggg tgcccttgcc ggacagcccg ttgaggtaca gcggcagggt cgaggtgagg
      361 gtcttgccct caccggtctt catctcggcc acccagccga agtggagggc ggcgccgccc
      421 atcagctgga cgtcgaagtg gcgctgcccg agcacccggg tcgccgcctc gcggcacacc
      481 gcgaaggcct cgagcatgat gtcgtcgagg tcctcgccgg cctcgagccg ctcgcggaac
      541 agcaccgtct ggtgcttgag ctcgtcgtcc gacatggcct ggtacgtcgg ctcgagcgcg
      601 ttgatctcgg gcaccaggcc ctggagcgcc cgcagacgct tgccttcacc ggttctgagg
      661 acgcgatcga ggatgcccat gcggttggcg agtgtatcgg cgcgccttca gcgcaccgag
      721 gcgatgcgga tcgcctacag cccgaggtcg aggggcagct gggcgccgtc gaaggcctcg
      781 gtgccgaggg ccagggcggc ggccgggccg ccgttgctgt gcatgtactc cagcgccgat
      841 gccaggtcgc tgtagttgtt cgatccggcc agcggggcga gggccagcgt cgacaggacg
      901 ccggccgccg cgccgtcggc gtcgagcagc gcgctgcccg agtcgccggg gatgccgggc
      961 gacacggtgt acacggggtg ggtccagccg ccggcggtgg tgccgaggcc gaggccggtc
     1021 ttgggcgaca gcgcacggat gccgaggcgc aggctggagt tcccgtagct gtgcacggtc
     1081 tcgccggccc cggtgccgtc ggtgttcagc gcggtgggac cgccccagta cgggatcgag
     1141 gggttgaccg cgccgtggtc ggccgggtcg atgcggacga gggcgaagtc gttgtggagg
     1201 caggtgtcgg gatccgtctc gccgacccgc tgcatggtct cccacgacga gtagacgagc
     1261 gtgccgggct gggatgcgcc ctcgatctcg acctgggtgg agccgtcggt gggcagcgac
     1321 ggggccaggc agccgtcggt gtcggtggcg gccccgccgt cgccggcgca gtgggcggcg
     1381 tagccgatga gcacgtcggc gccgtcggtg aagatgaagt tggcggtgca ctgggcaccg
     1441 ttggtgagca tctccgcacc gggcgtgatg gcggccacgt cggcgggggc ccactggacc
     1501 gtgctggtgg tgccgccctt gcccttcccc ttgccgttgc ccttgccctc ggcgtccgcc
     1561 ggggagatcc ccagcaccag gcccaccacg gcgacgagca ggacgaaggc gagggaggag
     1621 cgggaccggc gcatggacgg tggttcgacg gcagcggggc gaagtcctgc ggaggcctcc
     1681 ccgaggcagc agggggcgct gggtacgctg cccggggtgg cccgctcccg ctcgtgccga
     1741 ggccccggac gtcgcggccg ccgatgctgc cgactcgcca ccgtgctcgc cgtcggcttc
     1801 gtcgcggcct gctcgtcggg cgcggaggtg gcggcgccgg aggccggggt gaccgccccg
     1861 accaccaccg tgcgcaccgt gccgcccgcc gtggccggcg cgctccgagc cgaggtcccc
     1921 ccgcccgccg agccccgccg ggacgacctc gagcacgagg tgctcctcta cggggactcg
     1981 gtcgccctgc tcgtcgccga cgagctggcc gtcgagctcg acgcgcccct cgtggtcgac
     2041 gcggtcgact gccgtcgcct cgatgccgcg ttccccggac cgtgcggcgg cgtccccgcc
     2101 ggcaccgacg tggccagcgg gctcgacgac ctcgggccgg ccacggagcg gctcgacgac
     2161 ccggctgccg cgatcgccgt cgtcgtcatc gccaacaacg ccgccctggc cgcatccgac
     2221 ctcgacgccg ccatggccgc cctcggcgcc gtgccgcggg tctggtgggt caccacccgc
     2281 gtcgacggcc gggcctggca ggacccgaac aaccagctga tcgccgagct cgccgaccga
     2341 gacccgcggg ccgaggtgat cgactggttc gacgccagcg aggaccaggg ctggctcgtc
     2401 gacaacgtcc accccggcga cgagggccag gccgcgctgg ccgacctcat cgccgcccac
     2461 gtccgctgcg actgcacgcc atgaccgacc tcgccgaccc ccggaccgca cccgacgaca
     2521 gccggtggcc cctgcgcatc gaggagcccc cgccggccta cggcccctcc ctcggccggg
     2581 gcgtcgggta cctgcccggc atcgacggcc tgcgcgccct ggcggtcgtg gccgtgctcg
     2641 cctaccacct cgacgccagc tgggcgaccg gcggctacct cggcgtcgag gtcttcttcg
     2701 tcgtctccgg cttcctcatc accagcctcc tgctggccga gcaccgggag cacgaccgga
     2761 tcgacctgct cgccttctgg cggcgccggg cgcgccggct gctgccggcg gtgttcgtgc
     2821 tcgtggggct ggtgttcgcc tacgccgcgg tcgtgctgcc caccggcgag ctgcaccgct
     2881 tccggggcga cggcatcgcc agcctcctct acgtccagaa ctggcacgcc atcttcaccg
     2941 agcagccgta cttcgaggcc ttcgggcgcc cgtcgccgct gcgccacctg tggtcgctgg
     3001 cgatcgagga gcagttctac ctgctctggc ccctgctgct gccgatcggt ctgcggcgcc
     3061 tggggcggcg gcgcaccgtc atcgccgtgg ccggggcgat cctcgtctcg gtcgtgctca
     3121 tggccgccac ggccgacatc accgcgccgg agcgcgccta ctacggcacc gacacccggc
     3181 tcttcggcat cctctcgggc gccctcctgg ccttcgcctg gcgaccccag cgggcccggg
     3241 gcgacgtggg gccggcagcc cggcgggtca tcgagggcgc cgggatcgcc gccctggtcc
     3301 tcctcgcctg gcagcacgcc acccgcagcg agttcgacgg ctggacctac ccgtggggct
     3361 tcctcctcgt cgacgtgctg acgctggtca ccatcatcgc cgtgacccac ccggcgtcgc
     3421 gcctcgagcg ctcggtcggc agcggccccc tccaggccct cggtcgccgg tcgtactcga
     3481 tctacctgtg gcactggccg gtcatcgtct tcacgcgacc gggggtcgac tggggcctca
     3541 ccgggccggc cgcgctcgcc gcccggctcg cgctggtcgc ggtcctggcc gaggcctcct
     3601 accgcctcgt cgagcggccg gtgcgggacg gcacgctgca gcggtggctc gccggggcca
     3661 ccctcccgtc accggcggcg ctgctgcggc gtcgccgcgc cgtgggcatc gccgcgctcg
     3721 ccggcctcct gctcgccgtc accgtgatcg cggccccgcc cggcccgggg aacgaggtcg
     3781 ccgtcggcac cgtcgccgac cagccctcgg ccatcacgct gcccccggtc ccgccgacac
     3841 cgtccaccgc accgcccacg acggcggctg cggcaccggc cgacccccca ccgaccacgg
     3901 cgccgacgac g
//
LOCUS       DATISU010000381         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_870222, whole genome shotgun sequence.
ACCESSION   DATISU010000381 DATISU010000000
VERSION     DATISU010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870222"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VLR27_15055"
     CDS             <1..538
                     /locus_tag="VLR27_15055"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HSP04826.1"
                     /translation="TVKAVFSGVANAVVTADQLDVPLTYEGFQAIGSGMGSAGFIVHD
                     DTACMVNAAYVYSRFLSVESCGQCPPCKLGSGEITEHLQRLETGGGTAEDMQGIAHWL
                     GRVTDGNRCYLAVEEQIMVTSVVRAFAEEFVEHVDHGSCPRPRPIPLPKLVDLEGGQA
                     TYDETFWRKRPDWTYEPE"
     gene            576..1760
                     /locus_tag="VLR27_15060"
     CDS             576..1760
                     /locus_tag="VLR27_15060"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSP04827.1"
                     /translation="MASATSSADLGAPIAGIAEVLEGFEDPAALLGPDRSIVGANRAW
                     QVHGFTDATFVDGTQAPIDRVLRGEATQQAAVGRRSRRDGWQWYRSRVRSITGMPGIA
                     AVLTHRDVTDERRLHLRMAQSPVAHLELAPDGALLGVNERWEEIRGRPVGAELGMRWL
                     RDTPPEERRALLVRLDRPVPFQTTLTTIGSDERTCSIQLDLEPVLDAGEWIGWHASAT
                     DVTEVRALAAVADSAFTDDLTGLANRALFETTLTRSLARRSEEAPAAVLFLDLDGFKP
                     VNDLHGHAAGDAVLQLVAERIRSVLRPADLVARYGGDEFAVLLERADPEAAEHLGHRL
                     VESLRAPFSVAGHLIEIGTSVGAALTEDGDDVDTVIGRADAAMYAAKRAGGNRVAVSR
                     AD"
     gene            1794..2465
                     /locus_tag="VLR27_15065"
     CDS             1794..2465
                     /locus_tag="VLR27_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04828.1"
                     /translation="MALAGRSVQVVQLTLHDGGRIAVVHAAGASKHLESAVTEFDHPP
                     APARTRNWKKIGAITGGVFLVLMLIGAVAGDPEEQDVTIQEAADTRQVTTTAAPTTTA
                     APTTTVAPTTTAAPTTTTTTAAPTTTTTAAPTTTTTVPVPAAAPAALMPPIACGTDLQ
                     LAQDLVQEAGVWLSSSEDATGQGRNQVMDRNWTVLAAEPAPGTPIGEGDPVFYVVKDE
                     EFFGC"
     gene            2542..>2970
                     /locus_tag="VLR27_15070"
     CDS             2542..>2970
                     /locus_tag="VLR27_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04829.1"
                     /translation="MAAHVPPPPPVPPPPAVEDHQPGLPIRAWRTYRGWPTWLQVGLG
                     LLLVVAIGASGGGDDEQSVAIQAAAESEEVTTTDAPATTEAPTTTAAPTTTAAPTTTA
                     APTTTAAPTTTAPPVTAPPTTADPFAGESMSQKNARRSAAD"
BASE COUNT          455 a         1130 c         1012 g          373 t
ORIGIN      
        1 gaccgtgaag gccgtcttct ccggggtcgc caacgcggtg gtcaccgccg accagctcga
       61 cgtgccgctg acctacgagg gcttccaggc catcggcagc ggcatgggct cggccggctt
      121 catcgtccac gacgacaccg cctgcatggt caacgccgcc tacgtgtact cgcgcttcct
      181 ctcggtcgag tcctgcggcc agtgcccacc ctgcaagctc ggctccggcg agatcaccga
      241 gcacctccag cggctcgaga ccgggggcgg caccgccgag gacatgcagg gcatcgccca
      301 ctggctcggg cgggtgaccg acggaaaccg ctgctacctg gccgtggagg agcagatcat
      361 ggtcacgagc gtcgtccggg ccttcgccga ggagttcgtc gagcacgtcg accacggcag
      421 ctgcccccgg ccccggccca tcccgctgcc caagctcgtc gacctcgagg gtgggcaggc
      481 cacctacgac gagaccttct ggcgcaagcg gcccgactgg acctacgagc ccgagtgagc
      541 gtcctacgga ggcgctgacc cgggcaggga gcggcatggc ctccgccacg agctctgccg
      601 atctcggcgc gcccatcgcc ggcatcgccg aggtgctcga agggttcgag gacccggccg
      661 ccctgctcgg accggacagg agcatcgtcg gagccaaccg ggcgtggcag gtgcacggct
      721 tcacggacgc cacgttcgtc gacggcacgc aggccccgat cgaccgcgtc ctccgcgggg
      781 aggccaccca gcaggccgcc gtgggacgtc ggtcgcggcg cgacgggtgg cagtggtacc
      841 gctcccgggt gcgctcgatc acgggcatgc ccggcatcgc tgccgtgctc acccatcgtg
      901 atgtcaccga cgagcgccgc ctgcacctgc gcatggccca gtccccggtt gcccacctcg
      961 agctggcgcc ggacggcgcg ctgctcggcg tcaacgagcg gtgggaggag atccggggcc
     1021 ggcccgtggg cgccgagctg ggcatgcgct ggctgcggga caccccgccc gaggagcgac
     1081 gtgcgctgct cgtccgcctc gatcggcccg tgccgttcca gacgaccttg accaccatcg
     1141 gctccgacga gcggacctgc tccatccagc tcgacctcga accggtcctg gacgccggcg
     1201 agtggatcgg gtggcacgca tcggccaccg acgtgaccga ggtgcgcgcc ctggccgccg
     1261 tcgccgacag cgcgttcacc gacgacctga ccggcttggc caaccgtgcc ctgttcgaga
     1321 ccacgctcac ccgctcgctc gcccgccgca gcgaggaggc gccggcggcc gtgctgttcc
     1381 tggacctgga cgggttcaag ccggtgaacg acctgcacgg ccacgccgca ggagacgccg
     1441 tcctgcagct ggtcgccgag cgcatcaggt cggtgctgcg acctgcagac ctggtggccc
     1501 gctacggtgg cgacgagttc gccgtgctcc tggagcgcgc cgaccctgag gccgccgagc
     1561 acctgggcca ccgcctggtc gagtcgctcc gggcaccgtt ctcggtggcc ggccacctca
     1621 tcgagatcgg gacgtccgtc ggcgctgccc tgaccgagga cggcgacgac gtcgacaccg
     1681 tcatcgggcg agcggacgcc gcgatgtacg ccgcgaagcg cgccgggggc aaccgggtgg
     1741 cggtcagccg cgctgactag tccgctcggg tcaaatcggc attcacagtc cgaatggctc
     1801 tcgccggacg atcggtgcag gtcgtacaac tgaccctgca cgatggcggg cggatcgcgg
     1861 tcgtgcatgc cgctggggca tcgaagcatc tggagagcgc tgtgaccgag ttcgaccacc
     1921 ctcccgcgcc tgcgcgcacc cgcaactgga agaagatcgg cgccatcacc gggggcgtgt
     1981 tcctcgtcct catgctgatc ggcgccgtgg caggcgatcc ggaggagcag gacgtcacga
     2041 tccaagaggc cgcagacacc cgacaggtga cgacaaccgc cgcgccgacg accactgcag
     2101 cgccgacgac caccgttgcg ccgacgacca cggccgcccc gaccacgacc acgacgaccg
     2161 cggcgccgac cacgacgacg acggccgcgc caacgaccac gacgacagtg cctgtccccg
     2221 ctgcggcgcc cgccgcgctg atgcccccga tcgcgtgcgg cacggatctc cagctcgccc
     2281 aggacctcgt gcaagaagcc ggcgtgtggc tctcgagcag cgaggacgcg accggccagg
     2341 gtcgcaacca ggtgatggac cgcaactgga ccgtcctggc tgccgaaccg gcgcccggca
     2401 cgccgatcgg cgaaggcgac cccgtcttct acgtcgtcaa ggacgaagag ttcttcggct
     2461 gctgagcgca gcgggagcac cgagagccat caccttccac caccgccgga ccccggcggc
     2521 catccagaac aggagccttc catggctgcg cacgtcccac ctcctccgcc cgttcctcct
     2581 cctcccgccg tcgaggacca ccagccgggc ctcccgatcc gcgcgtggcg cacctaccga
     2641 gggtggccga cgtggctcca ggtgggcctc ggcttgctgc tcgtcgtggc gatcggcgcg
     2701 agcgggggtg gcgacgacga gcagtccgtc gcgatccagg cggcggcgga gagcgaggag
     2761 gtgacgacga cggacgcccc cgccaccacg gaggctccga cgaccaccgc tgcgcccacg
     2821 accacggcgg cgccgacgac caccgcggcc cccaccacga cggcggcgcc gaccaccacc
     2881 gccccgccgg tgaccgcgcc tccgacgacc gccgatccct tcgccggtga gtcgatgagc
     2941 cagaagaacg cacgccggtc ggcggcggac
//
LOCUS       DATISU010000382         3254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_543436, whole genome shotgun sequence.
ACCESSION   DATISU010000382 DATISU010000000
VERSION     DATISU010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543436"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..396)
                     /locus_tag="VLR27_15075"
     CDS             complement(16..396)
                     /locus_tag="VLR27_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04830.1"
                     /translation="MSDFAFRFDPTYRRLAVPFGVRPSTALVRVTDTQLDARFGPWRV
                     RTPLANIAGTQRTGPYTLPKTAGPAHLSMADRGITFATNPEAGLCIRFHEPVTGIDPF
                     RLIRHPGLTVTVADLDGLEQLLGV"
     gene            471..1487
                     /gene="argC"
                     /locus_tag="VLR27_15080"
     CDS             471..1487
                     /gene="argC"
                     /locus_tag="VLR27_15080"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HSP04831.1"
                     /translation="MISVGILGASGYAGSELLRLLAAHPELEVRLAGAESQAGTAVAD
                     LYPNLAAAYPDLGFTPATAEDCDGLDLVFLGLPHGLSQEIVPQLRGRVKWIVDAAADF
                     RLKDPSLYDQWYGQAHPAPGLLAEAAYGIPELFRDELTGADLVAVAGCYPTGAALALT
                     PFVRAGLVETTGVIVDAASGVSGAGRPPKAHTTFASASENMTAYGLLDHRHTPEIEQA
                     TGAQVLFTPHLVPMTRGILSTIYATPAAGASRDDLLSCLADTYADEPFVVVDDRIPQT
                     KAVAGSNCAHLTVRHDERTGRLLVISAIDNLVKGTSGQAIQCANVLAGLPETTGLPLT
                     GLLP"
     gene            1484..2368
                     /gene="argB"
                     /locus_tag="VLR27_15085"
     CDS             1484..2368
                     /gene="argB"
                     /locus_tag="VLR27_15085"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HSP04832.1"
                     /translation="MTATDRANVLLEALPYIQRFRQKVVVIKFGGNAMVDPALADSFA
                     ADIVLLHAVGLRPVVVHGGGPQIGELLARLGIESSFKDGLRVTDADTLDVARMVLVGK
                     VNRDIVGAINTHGPLAVGLSGEDAGLITSVARDPELGYVGDVESVNPAILHRLLAEEL
                     IPVVSTIGGSADGQAYNINADTVAGALAASLHAEKVIYLTDVPGLLEDVDDPSTLIGQ
                     TTVSELRRKVADGELTGGMIPKIDAAVAAVEHGVPAAHLLDGRLEHVVLLELFSDEGV
                     GTMITRHPHTGDTRGATP"
     gene            2365..>3254
                     /locus_tag="VLR27_15090"
     CDS             2365..>3254
                     /locus_tag="VLR27_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSP04833.1"
                     /translation="MTETQHAAAMGAPDDLDACPLMPTYGPPQVRFVRGQGTELWDGD
                     GTRYLDFLSGLAVVSLGHANPVVADAIAEQARTLCHVSNLFGTEPGWEVARTLDELLR
                     AGNPDASRYQTFFCNSGAESNEAAIKLARKFGGRGKHDVVCAFSSFHGRTLATLHATG
                     QPQKWEAFQPLPEGFRHVPWNDLDALEQALDASVAAVLLEPVQGEGGVNPATAEYFQG
                     VERLCRERGILFMVDEVQTGLGRTGEWFGFQHFGVQPDVVTMAKALGNGMPIGACAAK
                     VEVAAAFEPGDHATTFGGQPL"
BASE COUNT          459 a         1258 c         1091 g          446 t
ORIGIN      
        1 cgccggagcg aggggtcaga ccccgaggag ctgctccagg ccgtcgaggt cggcgacggt
       61 gaccgtgagg cccggatgcc ggatgaggcg gaacgggtcg atcccggtga cgggctcgtg
      121 gaaccggatg cacagcccgg cttcggggtt cgtggcgaac gtgatcccgc gatcggccat
      181 cgacaggtgg gccggccctg cggtcttggg cagcgtgtag gggccggtcc gctgcgttcc
      241 ggcgatgttc gccagcggtg tgcgcacccg ccacgggccg aagcgggcat cgagctgggt
      301 gtcggtgacc cgcacgagcg ccgtcgacgg ccggacgccg aaggggaccg ccagtcggcg
      361 gtacgtcgga tcgaagcgga acgcgaagtc ggacatggtg gctccgtacc cgagacgcac
      421 ccctcgaacc tgtttgcata gtcatgcaac ctgacggata ggctccgccc atgatctccg
      481 tcggcatcct gggtgcgtcc ggctacgccg ggtccgagct gctgcgcctg ctcgcggccc
      541 atccggagct cgaggtccgt ctcgccgggg ccgagagcca ggcgggcacc gccgtcgccg
      601 acctgtaccc gaacctggct gccgcctacc ccgacctggg cttcacgccg gcgaccgccg
      661 aggactgcga cggcctggac ctggtcttcc tcggcctgcc ccacgggctg agccaggaga
      721 tcgtcccgca gctgcggggc cgggtgaagt ggatcgtcga cgccgccgcc gacttccggc
      781 tcaaggaccc ctcgctgtac gaccagtggt acggccaggc ccatccggcg cccgggctcc
      841 tcgccgaggc cgcctacggc atccccgagc tgttccgcga cgagctgacc ggcgccgacc
      901 tcgtggccgt cgccggctgc tacccgaccg gcgcggccct cgccctcacg cccttcgtgc
      961 gggccggcct ggtggagacc accggcgtga tcgtcgatgc cgcctccggg gtgagcggcg
     1021 ccggacggcc cccgaaggcg cacacgacgt tcgcctcggc gtcggagaac atgaccgcct
     1081 acggcctgct cgaccaccgc cacacgcccg agatcgagca ggccaccggt gcccaggtgc
     1141 tgttcacccc gcacctggtg cccatgacgc ggggcatcct gtcgaccatc tacgccacgc
     1201 cggccgcggg tgcgagccgg gacgacctcc tgtcctgcct ggccgacacc tacgcggacg
     1261 agcccttcgt cgtcgtcgac gaccggatcc cccagaccaa ggccgtcgcc ggatcgaact
     1321 gcgcccacct gacggtgcgc cacgacgagc gcaccgggcg gctgctcgtg atctcggcca
     1381 tcgacaacct ggtcaagggc acgtccggtc aggccatcca gtgcgccaac gtcctcgccg
     1441 gcctcccgga gaccaccggc ctgcccctga ccgggttgct gccgtgaccg ccacggatcg
     1501 ggccaacgtc ctgctggagg ccctgcccta catccagcgc ttccgccaga aggtggtcgt
     1561 catcaagttc ggcggcaacg cgatggtcga ccccgcgctg gccgactcct tcgccgccga
     1621 catcgtcctc ctccacgccg tcgggctccg gccggtcgtg gtccacggcg gcggccccca
     1681 gatcggcgag ctgctcgccc gtctcggcat cgagtcctcg ttcaaggacg gcctgcgggt
     1741 caccgatgcc gacaccctcg acgtggcccg gatggtgctg gtcggcaagg tcaaccggga
     1801 catcgtcggc gccatcaaca cccacggccc cctcgccgtc ggcctgtccg gcgaggacgc
     1861 cggcctcatc acctcggtcg ctcgcgatcc cgagctcggc tacgtcggcg acgtcgagtc
     1921 ggtgaacccg gcgatcctgc accgcctgct cgccgaggag ctgatcccgg tcgtgtccac
     1981 catcggcggc agcgcggacg gccaggcgta caacatcaac gccgacaccg tcgccggcgc
     2041 cctggccgcc tcgctccacg ccgagaaggt catctacctc accgacgtgc ctggcctgct
     2101 ggaggacgtg gacgacccca gcacgctgat cggccagacg accgtgagcg agctgcgccg
     2161 caaggtcgcc gacggcgagc tcaccggcgg catgatcccc aagatcgacg ccgccgtggc
     2221 cgccgtggag cacggcgtgc ccgccgccca cctcctcgac ggccgcctcg agcacgtcgt
     2281 gctgctcgag ctgttctccg acgagggcgt cggcaccatg atcacccgcc acccccacac
     2341 cggggacacc aggggagcaa caccgtgacc gagacccagc acgccgccgc catgggcgcc
     2401 ccggacgacc tcgacgcctg cccgctgatg ccgacctacg gcccgcccca ggttcgcttc
     2461 gtccgcggcc aggggaccga gctctgggac ggcgacggca cccgttacct ggacttcctg
     2521 tccgggctcg ccgtcgtgtc cctcggccac gccaacccgg tggtggccga cgccatcgcc
     2581 gagcaggccc gcaccctctg ccacgtgtcg aacctgttcg gcaccgaacc cggctgggag
     2641 gtcgcccgca ccctcgacga gctgctgcgc gccggcaacc ccgacgcgtc ccgctaccag
     2701 acgttcttct gcaactcggg cgccgagtcc aacgaggccg ccatcaagct ggcccgcaag
     2761 ttcggcgggc ggggcaagca cgacgtggtc tgcgcgttct cgtcgttcca cggccgcacc
     2821 ctggcgacgc tgcacgccac cggccagccc cagaagtggg aggccttcca gccgctgccc
     2881 gagggattcc gccacgtgcc ctggaacgac ctcgacgcgc tcgagcaggc cctcgacgcc
     2941 tccgtcgccg ccgtgctcct cgagccggtg cagggcgagg gcggggtcaa cccggcgacc
     3001 gccgagtact tccagggcgt cgagcggctg tgccgcgagc gcgggatcct gttcatggtc
     3061 gacgaggtcc agaccgggct cggccggacg ggggagtggt tcggcttcca gcacttcggc
     3121 gtccagcccg acgtggtgac catggccaag gcgctcggca acggcatgcc gatcggcgcc
     3181 tgcgcggcca aggtcgaggt cgccgccgcc ttcgagccgg gcgaccacgc caccaccttc
     3241 gggggtcagc ccct
//
LOCUS       DATISU010000383         4576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_450862, whole genome shotgun sequence.
ACCESSION   DATISU010000383 DATISU010000000
VERSION     DATISU010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450862"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VLR27_15095"
     CDS             complement(<1..627)
                     /locus_tag="VLR27_15095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HSP04834.1"
                     /translation="MSTELVVIVTSIVVAFAAFAYMFALPRRGIWPRTWVAAAVVGLT
                     AAVGLVVLGRFGEIVGPFTVTELAIGLVAGAVWLVATHVGYSVISWVFPAFGDQVRDL
                     YRLGDEDTASRMVGPIAAMGVAEELLFRGVLQGVGGLVLALVAYTAVQVFERKWALAL
                     AALLGGVVWGALFAWRGGLVAPIAAHVLWTGMLTFVWTLGTAPRVQESG"
     gene            complement(624..1526)
                     /locus_tag="VLR27_15100"
     CDS             complement(624..1526)
                     /locus_tag="VLR27_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04835.1"
                     /translation="MTLAEPTGVEPVQAQDGFAAYAVRTRSGPHVTMQAGVVQSGRFI
                     TTTSRDSLKAKSVRAHRVAAATIEHDDGATEVLAGPTIAVDLRHPEAMFTDPAGALIA
                     GPATLRMAGDQLEQLLGYVEAAGKVPSGWLPHNRVLLVTRIDRTLRLEGFDLVDGSGD
                     WTPELPAPSELRRTSDRAEGSLPELPADVRHLVELEAPVRLGIDTPEGPLVLPARWQG
                     GDRFGVSAAALAQVQADLPGAGCATFDDSASRRPDRKRGVMLRGHLDLVEVDGAHATV
                     AIRTDRITAWDGFSASTTDVDDAP"
     gene            complement(1523..2044)
                     /locus_tag="VLR27_15105"
     CDS             complement(1523..2044)
                     /locus_tag="VLR27_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSP04836.1"
                     /translation="MTCFSTRNVSNGDVPVPRSAVWEVLTDPQCLTEMTPLLDGITVD
                     GDRWCWQLSGIRALGIEVAPSFTEQMTFDEPSRITYRHAPPAGQTERAGADGVYELTE
                     VGPDRTHLSIDITLHVDLPLPALSRRAVERVMAASIARTGEVFAERLYRHLGVATVGD
                     GMQSTTHKVRVSA"
     gene            complement(2093..4153)
                     /locus_tag="VLR27_15110"
     CDS             complement(2093..4153)
                     /locus_tag="VLR27_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HSP04837.1"
                     /translation="MTDERIWLEDIEGTDAMAWVRERNAEAEARLFADPDFDPLRQRI
                     LEVLEADDRIAYPSLHGDPAGDPTVHNFWTDASHRRGILRRTTWSDYRGGDPTWETVL
                     DVDALGAEEDESWVFHGADTRRSDRRRTLVDLSPGGSDASVTRELDLVDKAFVPEADG
                     GFVRPLAKGGLTWIDDDTVYVTTDHGPGSLTRSGYPRTVRRWRRGTPLADATVVFEGD
                     EADIVVGVAVGTVAGGRHHLFQRMLDFYTSETWIIPDGAGPDAAPVRLDVPIDVEVSV
                     HGPWLTLSLRKDWTVEGTTHPGGSLLVVDVDGFLAGTATPVPVFRPSAHRALAGHTWT
                     RSRLVLNVLEDVRNRIEVAHPDAEGTWTARPVTDVPDVWSLDVAAVDADTCDDLWLVA
                     NDFLTPESLYRVHVGDRDEAPELLRRAPERFDADGLAITQHFATSADGTRIPYFEVGP
                     EGGADAPRPTLLGGYGGFEVPRLPAYAPALGRAWLEHGGTYVLANIRGGGEYGPRWHQ
                     AGLRDQRPRVYEDFEAVALDLIDRGVTTPDRLGCTGGSNGGLLVGNMYVRSPELWGVI
                     VCQVPLLDMARYHLLLAGASWMAEYGDPDDPDDWAYLQGYSPYHQVDPAQDHPPILLT
                     TSTRDDRVHPGHARKMAALLAELGKDVTYWENTEGGHGGAADASQQATMQALLYVFLR
                     RRLV"
     gene            complement(4182..>4576)
                     /locus_tag="VLR27_15115"
     CDS             complement(4182..>4576)
                     /locus_tag="VLR27_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04838.1"
                     /translation="SADDLANAANGVQGMDGRDAFLDAWRPFLAAATDFAAATASGDA
                     AAAEEARSTMDAAPVQLATVFQEATAGNTAADLTDVLTTHVANLTAAIEAIVADDPAA
                     ATQARTAAQHASTVAAGLATAFDTAANQ"
BASE COUNT          606 a         1546 c         1822 g          602 t
ORIGIN      
        1 gcccgactcc tgcacgcggg gtgcggtgcc gagggtccac acgaacgtga gcatgccggt
       61 ccacagcacg tgggcggcga tgggggccac gaggccgccg cgccaggcga acagcgcccc
      121 ccacaccacg ccgccgagca gggcggccag ggccagggcc cacttccgct cgaagacctg
      181 cacggccgtg taggcgacga gcgcgaggac gaggccgccg acgccctgca ggacgccgcg
      241 gaacagcagc tcctcggcga cgcccatggc ggcgatgggg ccgaccatgc gggacgccgt
      301 gtcctcgtcg ccgaggcggt agaggtcgcg gacctggtcg ccgaaggcag ggaacaccca
      361 gctgatgacg gagtagccga cgtgggtggc gaccagccac accgcgcctg cgaccagccc
      421 gatggcgagc tcggtgaccg tgaacgggcc gacgatctcg ccgaagcgac ccaggaccac
      481 gagcccgaca gcggcggtga gcccgaccac ggcggcggcc acccaggtcc ggggccagat
      541 gccgcggcgg gggagggcga acatgtaggc gaacgcggcg aacgccacca cgatcgacgt
      601 cacgatgacg acgagctcgg tgctcacggc gcgtcgtcga cgtcggtcgt gctggccgag
      661 aacccgtccc aggccgtgat gcggtcggtg cggatcgcca ccgtggcgtg ggcgccgtcg
      721 acctcgacca ggtcgaggtg gccgcggagc atcacgccgc gcttgcggtc cggccggcgg
      781 ctggcgctgt cgtcgaaggt ggcgcagccg gcgccgggca ggtcggcctg cacctgggcc
      841 agggctgcag cggacacgcc gaagcggtct ccgccctgcc aacgggccgg cagcacgagg
      901 gggccctcgg gggtgtcgat gccgaggcgc acgggagcct cgagctcgac gaggtggcgc
      961 acgtcggcag ggagctcggg cagcgaaccc tcggcgcggt cgctggtgcg gcgcagctcg
     1021 gacggtgcgg gcagctccgg cgtccagtcg ccggacccgt cgaccaggtc gaagccctcg
     1081 agccgcaggg tgcggtcgat gcgggtcacg agcagaaccc ggttgtgggg cagccagccg
     1141 ctcggcacct tgcctgctgc ctccacgtag ccgagcagct gctcgagctg gtcgccggcc
     1201 atgcgcagcg tggcgggacc ggcgatgagc gcgccggccg ggtcggtgaa catggcctcg
     1261 gggtggcgca ggtcgacggc gatggtcggg ccggcgagga cctcggtggc gccgtcgtcg
     1321 tgctcgatcg tcgcggcggc gacgcggtgg gcgcgcacgg acttcgcctt cagcgagtcg
     1381 cgcgaggtcg tggtgatgaa gcggccgctc tggacgacgc cggcctgcat cgtcacgtgc
     1441 gggcccgacc gggtccgcac ggcgtaggcg gcgaagccgt cctgggcttg cacgggctcg
     1501 acgccggtgg gctcggcgag cgtcacgcgc tgactcggac cttgtgggtc gtgctctgca
     1561 tgccgtcgcc gacggtggcg acgccgaggt ggcggtagag gcgctcggcg aacacctcgc
     1621 cggttcgggc gatgctcgcc gccatcaccc gctccacggc acggcgcgac agcgccggca
     1681 gcggcaggtc gacgtgcagg gtgatgtcga tggacaggtg ggtgcggtcg ggaccgacct
     1741 cggtcagctc gtagacgccg tcggcaccgg cccgctccgt ctggccggca ggcggggcgt
     1801 ggcggtacgt gatgcgggac ggctcgtcga aggtcatctg ctccgtgaag gagggggcga
     1861 cctcgatgcc gagggcccgg atgccgctca gctgccagca ccagcggtcg ccgtccacgg
     1921 tgatgccgtc gagcagcggg gtcatctcgg tgaggcactg cggatctgtc agcacctccc
     1981 agacggcgct gcggggcacg gggacgtcgc cgttggacac gttgcgggtc gagaagcagg
     2041 tcacggagag ggctgtaccc gtctcgtcgg ggcgctcatg cgccgtgtga cgtcagacca
     2101 ggcgccgccg gaggaacacg tagagcagcg cctgcatcgt ggcctgctgg gacgcgtcgg
     2161 ccgcgccgcc gtgcccgcct tcggtgttct cccagtacgt cacgtccttc ccgagctcgg
     2221 cgagcagggc tgccatcttg cgggcgtggc cggggtggac gcggtcgtcg cgggtggacg
     2281 tggtcagcag gatcggcggg tggtcctggg cgggatcgac ctggtggtag ggcgagtacc
     2341 cctgcaggta ggcccagtcg tcgggatcgt cggggtcgcc gtactcggcc atccacgacg
     2401 cgccggcgag gagcaggtgg tagcgggcca tgtcgagcag ggggacctgg cagacgatga
     2461 ccccccacag ctccggggac cgcacgtaca tgttgccgac gagcagaccg ccgttgctgc
     2521 cgcccgtgca cccgagccgg tccggggtgg tgacgccgcg gtcgatgagg tcgagggcga
     2581 cggcctcgaa gtcctcgtag acgcgcgggc gctggtcccg caggccggcc tggtgccagc
     2641 gcgggccgta ctccccgccg ccgcggatgt tggccagcac gtaggtgccg ccgtgctcga
     2701 gccaggcccg tccgagcgcc ggggcgtagg ccggcaaccg gggcacctcg aatccgccgt
     2761 acccgccgag cagcgtgggg cggggcgcgt ccgcgccgcc ctcgggaccg acctcgaagt
     2821 aggggatgcg ggtgccgtct gcgctggtgg cgaagtgctg ggtgatcgcc agaccgtcgg
     2881 cgtcgaagcg ctcgggtgcc cgtcgcagca gctcgggtgc ctcgtcgcgg tcgccgacgt
     2941 ggacccggta gagcgattcg ggggtgagga agtcgttggc gaccagccag aggtcgtcgc
     3001 aggtgtcggc gtcgacggcc gcgacgtcga gggaccacac gtcggggacg tcggtgaccg
     3061 gacgggcggt ccacgtgccc tcggcgtcgg ggtgcgcgac ctcgatccgg tttcgcacgt
     3121 cctcgagcac gttcagcacc aggcgcgatc gggtccacgt gtggccggcc agggcccggt
     3181 gcgccgacgg gcggaagacg ggcacggggg tggcggtgcc ggccaggaag ccgtcgacgt
     3241 cgaccacgag cagcgagccc cccgggtggg tggtgccctc gacggtccag tccttgcgca
     3301 gcgagagcgt gagccacggc ccgtggaccg agacctcgac gtcgatgggc acgtcgaggc
     3361 ggaccggcgc ggcgtcaggc ccggccccgt cggggatgat ccaggtctcc gacgtgtaga
     3421 agtcgagcat ccgctggaac aggtggtggc gcccaccggc gacggtgccc acggcgaccc
     3481 ccacgacgat gtcggcctcg tcgccctcga agacgaccgt cgcgtcggcc agcggggtcc
     3541 cccgccgcca ccgccgcacg gtgcgggggt agccggaccg ggtgaggctc cccggcccgt
     3601 ggtcggtggt gacgtagacg gtgtcgtcgt cgatccaggt caacccgccc ttggccaacg
     3661 gtcggacgaa gcccccgtcc gcttcgggga cgaacgcctt gtcgaccagg tccagctcgc
     3721 gcgtcaccga cgcgtccgac ccgccggggg agaggtccac caacgtgcgg cgacggtcgg
     3781 agcgccgggt gtcggcgccg tggaagaccc aggactcgtc ctcctcggcg ccgagggcgt
     3841 cgacgtcgag gaccgtctcc caggtcgggt caccgccgcg gtagtccgac cacgtcgtgc
     3901 ggcggaggat gccgcgccgg tgggacgcgt cggtccagaa gttgtggacg gtcggatcgc
     3961 cggccgggtc gccgtgcagg gacgggtagg cgatgcggtc gtcggcctcg agcacctcga
     4021 ggatccgctg gcgcaggggg tcgaagtcgg gatccgcgaa cagccgggcc tcggcctcgg
     4081 cgttgcgctc ccggacccac gccatcgcgt cggtcccctc gatgtcctcc agccagatgc
     4141 gctcgtcggt cacggacgga ccctacgggc gccccggtgc gctactggtt cgctgccgtg
     4201 tcgaaggcgg tggcgaggcc ggcggcgacg gtggaggcgt gctgcgcggc cgtccgggcc
     4261 tgggtggccg ccgcgggatc gtcggcgacg atcgcctcga tcgctgcggt gaggttggcg
     4321 acgtgggtcg tcaggacgtc ggtcagatcg gcggcggtgt tgccggccgt ggcctcctgg
     4381 aagacggttg cgagctggac cggtgcggca tccatggtcg agcgcgcctc ctcggctgcc
     4441 gccgcatcgc cggatgcggt ggccgcggcg aagtcggtgg cggcggcgag gaaggggcgc
     4501 caggcgtcga ggaacgcgtc gcggccgtcc atcccctgca cgccgttggc ggcgttggcc
     4561 aggtcgtcgg cgctgc
//
LOCUS       DATISU010000384         6551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_451057, whole genome shotgun sequence.
ACCESSION   DATISU010000384 DATISU010000000
VERSION     DATISU010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451057"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VLR27_15120"
     CDS             complement(<1..593)
                     /locus_tag="VLR27_15120"
                     /inference="COORDINATES: protein motif:HMM:NF012286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HSP04839.1"
                     /translation="MTEQLDGGHVGIVGVGHVGMASAAALFHASMVSRLTLVDLDERR
                     AEGEAMDLMHGQALVGACDVRSGGLEALAGAAVVVMCAGVSQKPGETRIDLLARNADV
                     FRSVADGLDEHAPDAVVLIATNPVDIMTRIFAELSARPRERVIGTGTTLDSARLRALL
                     GRRYRVDPQSVHGYVLGEHGDTEMVPWSLVSIGGTTIR"
     gene            655..2409
                     /locus_tag="VLR27_15125"
     CDS             655..2409
                     /locus_tag="VLR27_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSP04840.1"
                     /translation="MWDDAAIARSQGELKMHDLVIRNGTIVDGTGADRFHGDVAVDDG
                     VITEIARDGESVGAGRQELDADGNLVAPGWVDIHTHYDGQVTWDPEMAPSSWHGATTV
                     VMGNCGVGFAPARPDERDFLIELMEAVEDIPGTALHEGIDWQWESFGEYLDALEAMPR
                     TIDVAAQVPHAALRAYVLGKDAHDAEVSADHLREMADLTAEAVRAGALGFSTSRTILH
                     TSKHGLIPGTTALPDELLAIGQGIAQGGPAVFQFVSDGLGTESVERQWIRELAALPGV
                     TVTYSLAQSPRDPHAYKDALDEAQALRAEGHTVVPQVPSRPTGMLFGLQSSFHPFMAH
                     PTYRPLWELSLDERVARLRDPEVRAQLLAEEPRTRNASALYLATNWEQIFRLGTDCDY
                     EPTPDSSVAATAAREGRSEQEVVLDWLLELDGKSLLFSPLGSYVEKNHDAIRSMIEHP
                     ASAVGLSDGGAHCGLICDASFPTYLLTHWTRDRTRGDKLPLELVVNKQTKATADVYGL
                     GDRGVLEVGKRADVNVIDLDALHLHVPHMIFDLPAGGRRLVQPVDGYRWTVQAGEVTF
                     VDGEATGARPGRVVRGRR"
     gene            complement(2415..4016)
                     /locus_tag="VLR27_15130"
     CDS             complement(2415..4016)
                     /locus_tag="VLR27_15130"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HSP04841.1"
                     /translation="MLRRIAVLLFFVAAAAGCTSGGDETSVDAEGLAVELELADGHAL
                     VGRLVIESDEPVRPEVEVVGTERSFDIPVADAANRHEIPVVGLRAEHDYQVSVSVGDE
                     VVQADLRTGPLPDQLPELRIATAEPAEVSEGFTVFNVLGLTEGDPPREGIAIAVDAEG
                     EVVWYHRTDHPIGDLRQLDDGTILHEYNDTGARRIDLFGATLEEWAGEIIMGPLRLDA
                     HDRQVVGEGATEVATDSMHHEVGMLDNGNLVAVSSELRTYEGFAGPLCGEGEDFDGSY
                     DLIVDVIVEYAPDGTVVAEYPLADHLDPVADARDQNVCGLPFDDVFPNWLYRAQGEAD
                     ALDWTHANAVVPDPSGEAFTVSVRHLDAILQIDRATGELLWRFGPGGDLEVEHPSDYS
                     SHQHAPEWQDDGTLLVYDNGNVRAPGPDDPADVEPTSRAVQYRIDADAGTAEVVWSYT
                     ATVDGEPAYASFVGDADRMANGNVLMTNGGYADRPDQVSAQIVEVVPDDSPAGGRVVL
                     DLRLDHPDQPIIYRAERVETFYPAG"
     gene            4123..4488
                     /locus_tag="VLR27_15135"
     CDS             4123..4488
                     /locus_tag="VLR27_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04842.1"
                     /translation="MDPVPGAPSDNTTAVAEIDDLREQGYGADFVVGEGGVVRCTKCR
                     HEERPEDVEVDVFRRMEGASDPDDMSAILAMTCRECGTKGIAMVGYGPRASADEGDLL
                     LAVDTPEDDGTDASVDNPA"
     gene            complement(4485..5225)
                     /locus_tag="VLR27_15140"
     CDS             complement(4485..5225)
                     /locus_tag="VLR27_15140"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSP04843.1"
                     /translation="MAARILTWNLQGRERPDLEAVAAVIADALPDVVALQEVQRSQAR
                     WLAARLGWSHVWRFKHWPLVVPAEGLAVLSPTPPADVAMVHLAHPFRFWSWRRRIAVR
                     AEVVTADGPLAVVAAHLGAGVDDAERSRQAELAVGLLRAGRLPVPRGCVVGDLNTLPG
                     SEVLATFATHGLRDAWGALHPEEPGPTNWSRGPRDGPPTQRLDYALVGDALEVVDVAV
                     PNAAEPGIHRYGALSDHLPVTVTVVQRR"
     gene            5301..>6551
                     /locus_tag="VLR27_15145"
     CDS             5301..>6551
                     /locus_tag="VLR27_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP04844.1"
                     /translation="MTDTAPRTADRSETEIIAELDEWLNENWDPDITVEEWWNRLGLS
                     GWGAPMWPEEWYGKDLNRSEAVAVQNRIGEFGALPAPGGLGLLLAGPTIIDHGSDEHK
                     RTLVKDIITGQKAWCQLFSEPGAGSDLAGINARAIKDGEEWIVNGQKVWTSGGHIADM
                     GMLIARTDPEQPKHQGITYFAVDMHQDGMDVRPLKEMTGRAMFNEVFMSDIRVPDEWV
                     IGGLNNGWRVANTTLMYERSGLGAGGGSAAASLASPGTVAGDLSKRVGDFVRNPDQRR
                     STGGGATAGRGGAKGLIEMAKAAGKVDDGPIRQDLMRLHTLHEIARMNNLRAKAARAA
                     KKDIPGLPNIMKLSMSEILRVNRETGMKILGPMATLHAYDGESRKKLDAATGNPFLGM
                     ITESALFSPGPAIYGGTDQIQKNII"
BASE COUNT          977 a         2469 c         2258 g          847 t
ORIGIN      
        1 cggatggtgg tgccaccgat cgacacgagg ctccacggga ccatctcggt gtcgccgtgc
       61 tcgccgagca cgtagccgtg caccgactgc gggtcgaccc ggtagcgccg gccgagcagc
      121 gcccgcaggc gagccgagtc cagcgtcgtg ccggtgccga tgacccgctc ccggggacgg
      181 gcgctgagct cggcgaagat ccgagtcatg atgtccaccg ggttggtggc gatcagcacg
      241 acggcgtccg gtgcgtgctc gtccagccca tcggccaccg agcggaacac gtcggcgttg
      301 cgggccagca ggtcgatgcg ggtctcaccc ggcttctgcg agacgccggc gcacatgacg
      361 accacggcgg caccggccag ggcctcgagg ccgcccgaac gcacgtcgca ggcgccgacg
      421 agggcctggc cgtgcatcag gtccatggcc tccccctcgg cccggcgctc gtcgaggtcg
      481 acgagggtga ggcgcgacac catcgaggcg tggaacagcg cggcggccga ggccatgccg
      541 acgtggccca ccccgacgat cccgacgtgg ccaccgtcca gctgctcggt catggcgcca
      601 gcgtcgcaga tcgccgcccg ccccggggtc cagccgctgg caacgtccgt ttccatgtgg
      661 gacgatgcgg cgatcgctcg ctcccagggg gaactgaaga tgcacgacct cgtcatccgc
      721 aacggcacca tcgtcgacgg caccggtgcc gaccgcttcc acggtgacgt ggccgtcgac
      781 gacggcgtca tcaccgagat cgcccgggac ggcgagtcgg tgggcgccgg ccgccaggag
      841 ctcgacgccg acggcaacct cgtcgccccc ggctgggtgg acatccacac ccactacgac
      901 ggccaggtca cctgggaccc cgagatggcc ccgtcgtcct ggcacggcgc caccacggtc
      961 gtcatgggca actgcggcgt ggggttcgcc ccggctcgac ccgacgagcg cgacttcctc
     1021 atcgagctga tggaggcggt cgaggacatc cccggcacgg ccctgcacga gggcatcgac
     1081 tggcagtggg agagcttcgg cgagtacctc gacgcgctgg aggcgatgcc ccgcaccatc
     1141 gacgtggccg cccaggtgcc ccacgccgcc ctgcgggcct acgtgctcgg caaggacgcc
     1201 cacgacgccg aggtctccgc cgaccacctg cgcgagatgg ccgacctgac cgcggaggcg
     1261 gtgcgggccg gcgcgctggg cttctccacc agccgcacca tcctccacac ctccaagcac
     1321 ggcctgatcc cgggcaccac cgcgctcccc gacgagctgc tcgccatcgg ccagggcatc
     1381 gcccagggcg ggccggccgt gttccagttc gtgtccgacg gcctcggcac cgagtccgtc
     1441 gagcgccagt ggatccgcga gctcgccgcc ctgcccggcg tcaccgtcac ctactccctg
     1501 gcccagtcgc cccgggaccc ccacgcctac aaggacgcgc tcgacgaggc ccaggcgctg
     1561 cgggccgagg gccacacggt ggtgccccag gtcccgagcc gtcccaccgg catgctcttc
     1621 ggcctgcaga gcagcttcca cccgttcatg gcccacccca cctaccggcc gctgtgggag
     1681 ctgagcctcg acgagcgggt cgcccgcctg cgcgaccccg aggtccgggc ccagctgctg
     1741 gccgaggagc cccgcacccg caacgcctcg gccctgtacc tggccacgaa ctgggagcag
     1801 atcttccgcc tgggcaccga ctgcgactac gagcccacgc cggactcctc ggtggccgca
     1861 accgccgccc gcgagggccg cagcgagcag gaggtcgtcc tcgactggct gctggagctg
     1921 gacggcaaga gcctgctgtt ctccccgctc ggctcctacg tcgagaagaa ccacgacgcc
     1981 atccgctcga tgatcgagca cccggcctcg gccgtcggcc tgtccgacgg cggcgcccac
     2041 tgcgggctga tctgcgacgc gtccttcccc acctacctgc tcacccactg gacccgcgac
     2101 cggacccggg gcgacaagct gcccctcgag ctggtggtga acaagcagac caaggccacc
     2161 gccgacgtct acggcctcgg cgaccggggc gtgctcgagg tcggcaagcg cgccgacgtc
     2221 aacgtcatcg atctcgacgc cctgcacctc cacgtgcccc acatgatctt cgacctgccc
     2281 gccgggggcc ggcggctggt gcagccggtc gacggctacc gctggacggt gcaggccggc
     2341 gaggtcacct tcgtcgacgg cgaggccacc ggtgcccggc ccggtcgggt ggtgcgcggt
     2401 cggcgctgag cgcctcagcc cgcggggtag aaggtctcga cgcgctcggc ccggtagatg
     2461 atcggctggt ccgggtggtc gaggcgcagg tcgagcacga cccggccacc tgccggggag
     2521 tcgtcgggca cgacctccac gatctgggcc gacacctggt cgggacggtc cgcgtagccg
     2581 ccgttggtca tcagcacgtt gccgttggcc atccggtcgg catcgcccac gaaggacgcg
     2641 taggcgggct ccccgtcgac ggtggcggtg taggaccaca cgacctcggc tgtcccggcg
     2701 tcggcatcga tgcggtactg gaccgcccgg ctggtgggct cgacgtcggc cgggtcgtcc
     2761 ggccccggcg ctcgcacgtt gccgttgtcg tagaccagca gggtgccgtc gtcctgccac
     2821 tccggcgcgt gctggtggga ggagtagtcg gacgggtgct cgacctcgag gtcaccgccg
     2881 ggcccgaacc gccacagcag ctcgcccgtg gcccgatcga tctgcaggat ggcgtcgagg
     2941 tggcgcaccg acacggtgaa ggcctcgccg ctcgggtccg gaaccaccgc gttggcgtgg
     3001 gtccagtcca gggcgtcggc ctcaccctgg gcccggtaca gccagttggg gaagacgtcg
     3061 tcgaagggca gcccgcacac gttctggtcg cgcgcgtcgg ccaccgggtc gaggtggtcg
     3121 gcgagggggt actcggcgac gacggtgccg tcgggggcgt actccacgat cacgtcgacg
     3181 atcaggtcgt agctgccgtc gaagtcctcg ccctccccgc agagcgggcc ggcgaacccc
     3241 tcgtaggtcc gcagctcgct gctcacggcc acgaggttgc cgttgtcgag catcccgacc
     3301 tcgtggtgca tggagtccgt cgccacctcg gtggccccct cccccacgac ctggcggtcg
     3361 tgggcgtcga gccggagcgg acccatgatg atctcgccgg cccactcctc gagcgtcgcc
     3421 ccgaacaggt cgatgcgcct cgcccccgtg tcgttgtact cgtgcaggat cgtgccgtcg
     3481 tcgagctggc gcaggtcgcc gatcgggtgg tccgtgcggt ggtaccagac cacctcgccc
     3541 tcggcatcga cggcgatggc gatcccctcg cggggcgggt cgccctcggt gagccccagc
     3601 acgttgaaga ccgtgaagcc ctcgctcacc tcggccggct cggcggttgc gatgcgcagc
     3661 tcgggcagct ggtcggggag cggcccggtg cgcaggtcgg cctggacgac ctcgtccccg
     3721 acggagaccg acacctggta gtcgtgctcg gcccgcaggc cgaccaccgg gatctcgtgg
     3781 cggttcgcag cgtcggcgac ggggatgtcg aagctgcgct cggtgccgac gacctcgacc
     3841 tcgggacgga ccggctcgtc gctctcgatc acgagccggc cgacgagggc gtggccgtcg
     3901 gccagctcga gctcgaccgc gagcccctcg gcgtcgaccg atgtctcgtc acccccgctc
     3961 gtgcagccgg ccgcggccgc gacgaagaag aggagcactg cgatccgccg gagcatcctc
     4021 cgagtgtggc ccacgggccg gtgcgatgca ggccgcccac gtccaggatc ggctcggacc
     4081 gggtagaacg ggacgatgac ctcccaacgt gaggaagacc acatggaccc cgtccccgga
     4141 gcaccctccg acaacaccac cgccgtcgcc gagatcgacg atctccgcga gcaggggtac
     4201 ggggccgact tcgtcgtcgg cgagggcggc gtggtccgct gcacgaagtg ccgccacgag
     4261 gagcggcccg aggacgtcga ggtcgacgtc ttccgtcgca tggaaggtgc gtccgacccg
     4321 gacgacatgt cggcgatcct ggccatgacg tgccgcgagt gcggcaccaa gggcatcgcg
     4381 atggtgggct acgggccccg cgcctcggcc gacgagggcg acctcctgct cgccgtcgac
     4441 acgcccgagg acgacggcac cgacgcgtcc gtcgacaacc cggcctaacg acgctgcacc
     4501 accgtcaccg tgacgggcag gtggtcggag agcgcgccgt accggtggat gcccggctcc
     4561 gccgcgttcg gcaccgccac gtcgaccacc tcgagggcat cgccgaccaa ggcgtagtcg
     4621 agccgctgcg tcggcggccc gtcccgtggc ccccgtgacc agttggtggg ccccggctcc
     4681 tccgggtgga gcgcacccca cgcatcccgg aggccgtggg tcgcgaaggt ggccagcacc
     4741 tccgagcccg ggagggtgtt gaggtccccg accacgcagc cccggggcac cgggaggcga
     4801 ccggcacgca gcagccccac ggccagctcg gcctgccggg accgctcggc gtcgtcgacg
     4861 cccgcaccga ggtgggcagc cacgaccgcg agcggtccgt cggcggtcac cacctcggcc
     4921 cggacggcga tccggcgccg ccacgaccag aaccggaacg ggtgggcgag gtgcaccatc
     4981 gccacgtcgg ccggtggggt cggcgacagc acggccaggc cctccgccgg caccaccagc
     5041 ggccagtgct tgaaccgcca cacgtgcgac cagccgagcc gggccgccag ccaccgggcc
     5101 tgggaccgct ggacctcctg gagggccacg acgtcgggca gggcgtcggc gatcacggcc
     5161 gccaccgcct ccagatcggg ccgctcccga ccctgcaggt tccaggtcag gatgcgggcg
     5221 gccacggccc catcgtgccc cacgctgcgc cgccccgaat gacttgaccg ccgggtcaag
     5281 tttgtggctc catgttggcc atgacggaca ccgcaccgcg caccgctgat cgatccgaga
     5341 ccgagatcat cgccgagctc gacgagtggc tcaacgagaa ctgggacccg gacatcaccg
     5401 tcgaggagtg gtggaaccgc ctgggcctgt ccggctgggg cgccccgatg tggcccgagg
     5461 agtggtacgg caaggacctc aaccgctccg aggctgtcgc cgtgcagaac cgcatcggcg
     5521 agttcggcgc cctccccgcc cccggcggcc tcggcctgct gctggccggg ccgacgatca
     5581 tcgaccacgg ctccgacgag cacaagcgca ccctggtgaa ggacatcatc accggccaga
     5641 aggcctggtg ccagctgttc tccgagcccg gcgccggctc cgacctcgcc ggcatcaacg
     5701 cccgggcgat caaggacggc gaggagtgga tcgtcaacgg ccagaaggtg tggacctccg
     5761 gcggccacat cgccgacatg ggcatgctca tcgcccgcac cgaccccgag cagcccaagc
     5821 accagggcat cacctacttc gccgtcgaca tgcaccagga cggcatggac gtccgcccgc
     5881 tcaaggagat gacgggccgg gccatgttca acgaggtgtt catgtccgac atccgggtgc
     5941 cggacgagtg ggtcatcggc ggcctcaaca acggctggcg cgtcgccaac accacgctga
     6001 tgtacgagcg ctccggcctc ggcgccggcg gcggctcggc cgcggcctcc ctcgcctccc
     6061 ccggcaccgt cgccggcgac ctgtccaagc gggtcggcga cttcgtgcgc aaccccgacc
     6121 agcgccgctc caccggcggc ggcgccaccg ccggccgtgg cggcgccaag ggcctcatcg
     6181 agatggccaa ggcggccggc aaggtggacg acggccccat ccgccaggac ctcatgcggc
     6241 tgcacacgct gcacgagatc gcccgcatga acaacctgcg ggccaaggcg gcccgggcgg
     6301 ccaagaagga catccccggc ctgcccaaca tcatgaagct gtcgatgtcg gagatcctgc
     6361 gggtcaaccg cgagaccggc atgaagatcc tcggcccgat ggccacgctg cacgcctacg
     6421 acggcgagag ccgcaagaag ctcgacgccg ccaccggcaa cccgttcctc ggcatgatca
     6481 ccgagtccgc cctgttctcg cccggccccg ccatctacgg cggcaccgac cagatccaga
     6541 agaacatcat c
//
LOCUS       DATISU010000385         6364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_917581, whole genome shotgun sequence.
ACCESSION   DATISU010000385 DATISU010000000
VERSION     DATISU010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917581"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3075)
                     /gene="hrpA"
                     /locus_tag="VLR27_15150"
     CDS             complement(<1..3075)
                     /gene="hrpA"
                     /locus_tag="VLR27_15150"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HSP04845.1"
                     /translation="MGPTTPSPATLRERLDQASVADAHRLGRRLGNLKRKKGEARQAA
                     LDSIAAELDRSIAAVETRRSTGVVPTYPDDLPITARRDELLEVLRDHQVVIVAGETGS
                     GKSTQLPKMCLELGRGVRGLIGHTQPRRIAARSVSERVAEELGVEVGGQVGYAVRFTD
                     QVGPDTRLKVMTDGILLNELQRDRLLLGYDTIIVDEAHERSLNIDFILGYLKELLPKR
                     PDLKVVVTSATIDTERFAEHFADERGPAPIIEVSGRTYPVEQRYRPDEDGQPPQDTVQ
                     AVADAVQELVREGPGDVLVFASGEREIRDMADAVEGLRLPGTEVLPLFARLSSAEQHR
                     VFARHTKRRVVIATNIAETSITVPGIRYVVDPGTARISRYSKRTKVQRLPIEAISQAS
                     ADQRAGRCGRVAPGICIRLYAEDDYDSRPEFTEPEILRTNLASVILQMTALGLGDVES
                     FPFVEPPDRRNIADGVTLLEELGALEPDRPADDRRLTEIGRKLARLPLDPTLGRMVLE
                     AERNRCVHEVMVIVAGLSIQDPRERPTGQEERAGQLHARFRVPGSDFLGYLALWDHVK
                     ERQRELSSSRFRRECRDELLHFVRLREWQDVYTQIRQVVRSLKIGIDRDGADADAIHR
                     ALLAGLLANVGNRDDSEEPTPAKGPSGRRGPKGPVEYVGPRNARFALGRGSVLSKSPP
                     RWVMVAEIVETNRLWARTAATVQPSWIEQAAGHLLTRTYEEPHWNPDRGSAQVIERVR
                     LRALTIVEGRAINLDRVDRAMARELFILHALVRGEWESHHGFVQRNADRVAEVRAMED
                     RARRRDILVGEDEVFDVFDAHIPDDVTDVRRFDRWWRDVQQDQPDLLDLTVADLVVPG
                     TTDVDDAAFPDRWDDAPAEIRIDYEFEPGRASDGTTFEVPVAVLNRTDPEPFEWLVPG
                     LRHELVTSLIKALPKQVRRSLVPAPDRAAELLERVSPDDGPLREVVAAELSRMAGATV
                     TPDMWADLVLPGHLRPRYRVVGEGRTLAEGDDLRELERRLRAHVRATVVSSAP"
     gene            complement(3125..4228)
                     /locus_tag="VLR27_15155"
     CDS             complement(3125..4228)
                     /locus_tag="VLR27_15155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HSP04846.1"
                     /translation="MLDWVPRWLRVAFGVLVMALVVWFLVIPQFGQAERALDAITGVH
                     MGYVLLGGVLVAASIAGQAELVRAILPPGERPSLSDMVRMELATTAVSHTVPGGTAAG
                     TALGYRLLTQAGVQGTSAGFALGVRGIGSALVLNVILWLALLVSIPTTGFDGRYTTAA
                     VLGSALLTAVGAVVVGLLRAPEATTRVVARILRPIPFVDAEGVPRVIGRLSEQLRELV
                     SDRSLALRSVAWASAYWLGQAAALWVLLLAFGWTGHPVSVMVAFGLVNVLAALPVTPR
                     GLGVLEAVLIPMLVGFGAPAYVATLGVVSWRLLTFWAPIPLGTIAYVSIRVRQPGVQG
                     DAEDAADELGAVGREHREPWTEWADRHAIRRRR"
     gene            4326..5339
                     /locus_tag="VLR27_15160"
     CDS             4326..5339
                     /locus_tag="VLR27_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04847.1"
                     /translation="MLTLRYRRDELMEDHDYAEPQVIAGRRMHGGFLADGTYQPPRTK
                     VRGPAIDAWTKALRDRGGELFDADASLLDGVRLPNVDQQRVLLRNGLGETFWNNLTII
                     GKIEAKGRLLADITFPDLQDVIVEDISDMAIGHLNDGLLVAHGLDEGGLPEQGIGGHD
                     EMWFAARDLAFGTDAHPDVDPPENIARPDDERRIPELRPEIEGLVSFLANLLLIEFRA
                     ELGFSETQAVLRTPDLWGDRAEQAAEAAEIIGRIRTDEEIHVSSLRLYLGEIRACTFR
                     TVDGGTIGGAEVIDRFWSDLVRWAVVDKPALEADQQRRVLEDRIRAHAEADRVLQEFD
                     AAA"
     gene            complement(5344..>6364)
                     /locus_tag="VLR27_15165"
     CDS             complement(5344..>6364)
                     /locus_tag="VLR27_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSP04848.1"
                     /translation="ADLIRQTIRLGAPPWPTVDPFLFCVHHVDAYPAGNLHLGPDAEL
                     QGRAIGQDFANVDGWNMYHGSVVPGFPSHPHRGFETITYVRRGLVDHADSLGAAARYG
                     KGDVQWMTAGKGIQHAEMFPLLDQSGPNPMELFQIWLNLPAEDKMVEPSYKMLWDDEI
                     PRVPVDGDGEVTVIAGAIPGPSGLVVPPSPAPDSWAARSDTDVALWHVRLRAGASVVL
                     PPAAYEDTVRVLYVFDGDGVSVDGQDHDVPTGIVVDSGQEAPLQALGEGVDVLVMQGR
                     PIGEPVAQYGPFVMNTKAEIQQAFEDYQRDHFGGWPWDRSDPVHGRDPARFARHADGR
                     EERAS"
BASE COUNT          886 a         2277 c         2353 g          848 t
ORIGIN      
        1 cggcgcgctg gacaccacgg tggcgcgcac gtgggcccgg agccggcgct ccagctcccg
       61 caggtcgtcg ccctcggcca gggtccggcc ctcgcccacc acgcggtagc gggggcggag
      121 gtgcccgggc agcacgaggt cagcccacat gtccggggtc accgtcgcac cggccatgcg
      181 gctcagctcg gccgccacga cctctcgcag cggcccgtcg tcggggctga cacgctcgag
      241 cagctcggcg gcccggtcgg gggccgggac gagcgagcgc cgcacctgct tgggcagggc
      301 cttgatcagg ctggtcacca gctcgtggcg caacccgggg accagccact cgaacggctc
      361 gggatcggtg cggttcagca cggcgaccgg cacctcgaag gtggtgccgt ccgaggcccg
      421 gcccggttcg aactcgtagt cgatgcggat ctcggccggc gcgtcgtccc accggtccgg
      481 gaaggcggcg tcgtcgacgt cggtggtgcc gggcacgacg aggtcggcga cggtgaggtc
      541 gagcaggtcg ggctggtcct gctggacgtc gcgccaccag cggtcgaagc gacggacatc
      601 ggtgacgtcg tccgggatgt gggcatcgaa gacgtcgaac acctcgtcct cgccgacgag
      661 gatgtcccgg cgccgggccc ggtcctccat ggcccggacc tcggcgaccc ggtcggcgtt
      721 gcgctggacg aagccgtggt ggctctccca ctcgccccgc acgagcgcgt ggaggatgaa
      781 cagctcgcgc gccatggccc ggtcgacccg gtcgaggttg atcgcccggc cctccacgat
      841 ggtgagggca cggagccgga cccgctcgat gacctgggcg gaaccccggt cggggttcca
      901 gtgcggctcc tcgtaggtgc gggtcagcag gtgcccggcg gcctgctcga tccacgacgg
      961 ctggacggtg gcggccgtgc gggcccacag ccggttggtc tccacgatct cggccaccat
     1021 cacccagcgg ggcgggctct tcgacagcac cgagccacgg ccgagggcga agcgtgcgtt
     1081 gcgggggccg acgtactcga ccgggccctt cgggccccgg cgcccggacg gccccttcgc
     1141 cggcgtcggc tcctcgctgt cgtcgcggtt gcccacgttg gcgaggagcc cggcgagcag
     1201 ggcgcggtgg atggcgtcgg cgtcggcgcc gtcgcggtcg atgccgatct tcaggctgcg
     1261 caccacctgg cggatctggg tgtagacgtc ctgccactcc cggagccgca cgaagtgcag
     1321 cagctcgtcg cggcactccc ggcggaacct cgacgacgac agctcccgct ggcgctcctt
     1381 cacgtggtcc cacagggcca ggtagccgag gaagtcggaa ccgggcaccc ggaaccgggc
     1441 gtggagctgg ccggcgcgct cctcctggcc ggtggggcgc tcgcgcgggt cctggatgga
     1501 caggccggca acgatgacca tgacctcgtg cacgcaccgg ttgcgctcgg cctcgagcac
     1561 catccgaccc agcgtggggt cgagcggcag gcgcgccagc ttgcggccga tctcggtgag
     1621 gcggcggtcg tcggcggggc ggtcgggctc gagggcaccg agctcctcga ggagggtgac
     1681 gccgtcggcg atgttgcgcc ggtcgggcgg ctcgacgaac gggaacgact cgacgtcgcc
     1741 gaggccgagt gccgtcatct gcaggatgac cgaggccagg ttggtccgca ggatctcggg
     1801 ttcggtgaac tcggggcggc tgtcgtagtc gtcctcggcg tagaggcgga tgcagatgcc
     1861 cggtgccacc cggccgcagc gaccagcgcg ctggtcggcg ctggcctggg agatcgcttc
     1921 gatcggcagg cgctgcacct tggtgcgctt gctgtagcgg gagatgcgtg ccgtgcccgg
     1981 atccacgacg tagcggatcc ccgggaccgt gatcgacgtc tcggcgatgt tggtggcgat
     2041 gaccacccgg cgcttggtgt ggcgggcgaa cacccggtgc tgctcggccg aggagagccg
     2101 ggcgaacagc ggcaggacct cggtgccggg caggcgcagc ccctcgaccg cgtcggccat
     2161 gtcgcggatc tcccgctccc ccgaggcgaa cacgagcacg tcgccgggcc cctcgcgcac
     2221 gagctcctgc acggcgtcgg ccacggcctg gacggtgtcc tggggcggtt ggccgtcttc
     2281 gtcgggtcgg tagcgctgct cgaccgggta cgtgcggccc gacacctcga tgatcggggc
     2341 cggaccccgc tcgtcggcga agtgctcggc gaatcgctcg gtgtcgatgg tggcggaggt
     2401 gacgacgacc ttgaggtccg gccgcttcgg cagcagctcc ttgaggtagc cgaggatgaa
     2461 gtcgatgttg aggctgcgct cgtgggcctc gtccacgatg atcgtgtcgt agccgagcag
     2521 cagccgatcc cgctggagct cgttcagcag aatgccgtcg gtcatgacct tgaggcgggt
     2581 gtccgggccg acctggtcgg tgaagcgcac cgcgtagccg acctggccgc cgacctcgac
     2641 gccgagctcc tcggccacgc gctcggacac cgagcgggct gcgatccggc ggggctgcgt
     2701 gtgcccgatg agcccgcgca ccccgcggcc gagctcgagg cacatcttgg gcagctgggt
     2761 gctctttccc gatccggtct cgccggcgac gatcaccacc tggtggtcgc gcaggacctc
     2821 gagcagctcg tcgcgtcggg cagtgatcgg caggtcgtcg gggtaggtcg gcaccacgcc
     2881 ggtcgaccgc cgggtctcca ccgcggcgat ggagcggtcc agctcggcgg cgatcgagtc
     2941 gagcgccgcc tggcgggcct cacccttctt gcgcttcagg ttcccgagcc ggcgaccgag
     3001 ccggtgcgcg tcggccaccg aggcctggtc gaggcgctcg cggagggtgg cgggagacgg
     3061 ggtggtgggt cccatgggca gccgaccagg atgccaccgg tcggcggtcg tgcgaccgtg
     3121 cggttcagcg ccgccggcgg atggcgtggc ggtcggccca ctccgtccac ggttcgcggt
     3181 gctcccggcc gaccgcgccg agctcgtcgg ccgcatcctc cgcatcgccc tgcacgccgg
     3241 gctggcggac acggatcgac acgtaggcga tggtcccgag cgggatcggt gcccagaacg
     3301 tgagcaggcg ccagctgacg accccgaggg tggcgacgta ggcgggcgcc ccgaagccga
     3361 cgagcatcgg gatgagcacg gcctcgagca cccccaggcc tcgcggcgtc acggggaggg
     3421 cggcgaggac gttgaccagc ccgaacgcca ccatcaccga caccgggtgg ccggtccagc
     3481 cgaacgccag cagcaggacc cacagggcgg cggcctggcc gagccagtag gcggaggccc
     3541 acgccaccga ccgcagggcg aggctgcgat ccgagaccag ctcgcgcagc tgctccgaga
     3601 gccggccgat gactcgtggc accccctcgg cgtccacgaa cgggatgggt cgcaggatcc
     3661 gggccacgac ccgggtggtg gcctccgggg cccgcagcaa cccgacgacg acggctccga
     3721 cggcggtgag cagcgccgac cccaggaccg cggccgtggt gtagcggccg tcgaagccgg
     3781 tggtcgggat cgacaccagc agggcgagcc agaggatgac gttgaggacg agcgccgagc
     3841 cgatgccgcg caccccgagc gcgaaccccg ccgacgtgcc ctgcaccccg gcctgggtga
     3901 gcagccggta gccgagggcg gtgccggcgg cggtcccgcc cggcacggtg tggctcaccg
     3961 cggtcgtggc cagctccatg cggaccatgt ccgacaggct cgggcgctcc cccggcggca
     4021 ggatcgcccg caccagctcg gcctgcccgg cgatggaggc ggcgaccagc accccgccga
     4081 gcagcacgta gcccatgtgc acgccggtga tggcgtcgag ggcccgctcg gcttgaccga
     4141 actgcgggat caccaggaac cacacgacga gggccatcac gagcacgccg aaggccacac
     4201 gcaaccaccg tgggacccag tcgagcatcg gcacagcctg gcagcgggcc gcccggtttc
     4261 ccccacgtgg cccgccgagt accttgaccg acgggtcaag tcgacgacgc agggggtccc
     4321 cgaccgtgct cacgctccgc taccgccgtg acgagctgat ggaagaccac gactacgccg
     4381 agccgcaggt gatcgcgggc cggcgcatgc acggcggctt cctcgccgac ggcacctacc
     4441 agccgccgcg caccaaggtc cggggtccgg ccatcgacgc ctggaccaag gccctgcgcg
     4501 accgcggcgg cgagctgttc gacgccgacg cgtcgctcct cgacggcgtc cgcctgccca
     4561 acgtcgacca gcagcgggtc ctgctccgca acggcctcgg cgagacgttc tggaacaacc
     4621 tgaccatcat cggcaagatc gaggccaagg gccgcctcct cgccgacatc accttcccgg
     4681 acctccagga cgtgatcgtc gaggacatct ccgacatggc gatcggccac ctcaacgacg
     4741 gcctcctcgt ggcccacggc ctggacgagg gcggcctgcc cgagcagggc atcggcggcc
     4801 acgacgagat gtggttcgcc gctcgcgacc tcgcgttcgg caccgacgcc caccccgacg
     4861 tcgacccgcc cgagaacatc gcccgccccg acgacgagcg ccgcatcccc gagctccgcc
     4921 ccgagatcga gggcctggtg tcgttcctgg ccaacctgct gctcatcgag ttccgcgccg
     4981 agctcggctt ctccgagacc caggccgtgc tccgcacgcc cgacctgtgg ggcgaccgcg
     5041 ccgagcaggc cgccgaggcc gccgagatca tcgggcgcat ccgcaccgac gaggagatcc
     5101 acgtctcctc gctccggctc tacctcggcg agatccgcgc ctgcacgttc cgcaccgtcg
     5161 acggtggcac catcggtggc gccgaggtca tcgaccgctt ctggagcgac ctcgtccgct
     5221 gggcggtggt cgacaagccg gccctcgagg ccgaccagca gcgccgggtg ctcgaggacc
     5281 gcatccgggc ccacgccgag gccgaccggg tgctgcagga gttcgacgcc gccgcttgag
     5341 cgttcagctg gctcgctcct cgcgcccgtc agcgtggcgg gcgaagcggg ccgggtcgcg
     5401 cccgtgcacc gggtcgctgc ggtcccaggg ccagccgccg aagtggtcgc gctggtagtc
     5461 ctcgaaggcc tgctggatct cggccttggt gttcatgacg aaggggccgt actgggcgac
     5521 cggctcgccg atggggcggc cctgcatcac cagcacgtcg acgccctcgc cgagagcctg
     5581 caacggcgcc tcctggccac tgtccacgac gatgccggtg ggcacgtcgt ggtcctgtcc
     5641 gtcgacggac acgccgtcgc cgtcgaacac gtacaggacc cggacggtgt cctcgtaggc
     5701 cgcgggcggc agcacgacgg acgcgccggc gcgcaggcgg acgtgccaca gcgcgacgtc
     5761 ggtgtcgctg cgggcggccc acgagtccgg cgccggcgac gggggcacca cgagcccgga
     5821 cggaccgggg atggcaccgg cgatgacggt gacctcgccg tcaccgtcga ccggcacgcg
     5881 ggggatctcg tcgtcccaga gcatcttgta gctgggctcg accatcttgt cctcggccgg
     5941 caggttcagc cagatctgga acagctccat cgggttgggc ccggactggt cgagcagcgg
     6001 gaacatctcg gcgtgctgga tgcccttgcc ggcggtcatc cactgcacgt cgcccttgcc
     6061 gtagcgggcg gcggcgccga gggagtcggc gtggtcgacg aggccgcgac gcacgtaggt
     6121 gatggtctcg aagccccggt gcgggtgcga cgggaacccg ggcacgaccg acccgtggta
     6181 catgttccag ccgtcgacgt tggcgaagtc ctggccgatg gcccgcccct gcagctcggc
     6241 gtcgggaccg aggtgcaggt tgcccgccgg gtaggcgtcg acgtggtgga cgcagaacag
     6301 gaacggatcg accgtgggcc acggcggcgc gcccaggcgg atggtctgtc ggatcaggtc
     6361 ggcc
//
LOCUS       DATISU010000386         7690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_78659, whole genome shotgun sequence.
ACCESSION   DATISU010000386 DATISU010000000
VERSION     DATISU010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78659"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VLR27_15170"
     CDS             complement(<1..1142)
                     /locus_tag="VLR27_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSP04849.1"
                     /translation="MTTGSDGEHGEDISREQLVALEAEVAALRQRLQDAPKRVRTLEE
                     RLLETKGQLSQAVSQNEKLSYTLREAREHIAALREEVDKLTQPPSAYGTFLRMNDDGT
                     ADVHAGGRKMRVAVHPELADDVDALPQGAEVVLNESFSIILSRSPEGQGEVVTLKEIL
                     EDRRRALVVGRADEERVVELVDELVGERLRSGDTMLLDPRSGLLLEKLPRPEVEELVL
                     EEVPDVGYDDIGGLDDQIEQIADAVELPFLHQELFAEFELPAPKGILLYGPPGCGKTL
                     IAKAVANSLAKKVAESTGDEARSFFLNIKGPELLNKYVGETERQIRLVFQRAREKSEE
                     GWPVIVFFDEMDSMFRTRGSGISSDMESTIVPQLLAEIDGVESLKNV"
     gene            1335..1568
                     /locus_tag="VLR27_15175"
     CDS             1335..1568
                     /locus_tag="VLR27_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HSP04850.1"
                     /translation="MKVWIDQDLCTGDGLCEEIAPDVFALLDDGLAYVKDGDKILSEP
                     GGAEAVAPVPAGQEEAVIESAEECPGECIFIEV"
     gene            complement(1616..2917)
                     /locus_tag="VLR27_15180"
     CDS             complement(1616..2917)
                     /locus_tag="VLR27_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HSP04851.1"
                     /translation="MTGTDLDVAVAEARARYAEARPISAGWSDRAAKVMPGGNTRSIL
                     QFEPFPFRVERAAGKHLWDVDGHRYVDLLGNYTAGLLGHSPAPVLAAAKEALSTGWSL
                     GAVHEDEVRLAELLVERFPSLEQVRFTNSGTEANLMALAAATHHTGRRRIVVFRNGYH
                     GGVLTFGRDDDPVNVPHEWLLCDYNDVGGAEAAFAEHGSEIACVLVEPMQGSGGCIPA
                     DPDFLQALHRLCRDAGALLVFDEVMTSRSSAGGAQQLLGITPDLTTLGKYLAGGLTFG
                     AFGGRADVMAHFDAGQGGRLGHAGTFNNNVASMAGGVAALSELLTGSVLDDLHARGEE
                     MRAALNEVLASAGLPMCATGAGSLMNVHGTPGPVSRAQDLRGADDRLKELFFFHCLEA
                     GFYVARRGFVALSIEITGADIEAFLDVVRAFDPTGARNGRP"
     gene            complement(2943..4388)
                     /locus_tag="VLR27_15185"
     CDS             complement(2943..4388)
                     /locus_tag="VLR27_15185"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HSP04852.1"
                     /translation="MVDLRRPRRTSPLGAAFEVVSPERVRNLLPVAIVAISSGRVVLV
                     LLVAGAISLIAGVLGWLRRLWSFDGDVLHLDEGVFVRNQRRIPVDRIQHVELERSLRH
                     QIFGLAAVRVETAGGSGAELRLDAVGRDEAEALRSQVLDALRARAAVPEGSGGDGEGW
                     DAARHGPMPPPPPAPREVLVRLPPGRLLLAGITGPEVVAVLASLVFAVDALADLGIDP
                     DEIGRVDLGRTAIALLVLVGVPVWFLIAGLIAVVRKWDLTASISGDELRVTYGLLRKH
                     EFVVRTSRVQDARVAQRLLLRPFGRADLRVRSAASGGGDASQVDIPLLDAAEIDRVLT
                     RVLPAAVPRPRLHPAPSGARRRSLVRGAAGALLGASALSVLAVVTSRWWFVPAAIVLV
                     LGPLLGELAYRGLGWAEDGGVQHSRSGALARRTAIVPSARVQSAAVVSTLFQRRRGLG
                     SVRLDLASSAVGVLDRDAGECGHIAAAVTAD"
     gene            complement(4390..4884)
                     /locus_tag="VLR27_15190"
     CDS             complement(4390..4884)
                     /locus_tag="VLR27_15190"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HSP04853.1"
                     /translation="MTEPDADDPGIGDLVPLERRVLSLWRLRATIALAVWVALVLAAG
                     AGVGQALPGALAALVLAVVGGLLVRWWTSLVWQAWQFRVGDGALHLRHGVLTRRQSTI
                     PYHRVQHIDLEAGPLERRMGLTSLILRTASASSDSTVPGIDAAEAEALRARILALVGT
                     GDAV"
     gene            complement(4881..6047)
                     /locus_tag="VLR27_15195"
     CDS             complement(4881..6047)
                     /locus_tag="VLR27_15195"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HSP04854.1"
                     /translation="MNLLDLILLGTGVLAMVGGYRLGFLTRTASWVGMGLGLFLGART
                     LPWLVEQFEHESPASLLLLGGIVLLVGSFLGQAVGVVVGSRLRVNLGNSEWTIVDHAF
                     GAVAGVLGMVIAVWLLLPTMSEVPGAFSSQARNSVIARSVDRILPAPPDALVALRRLV
                     GEDQFPRVFDAMRPAPDVGPPPTDAQITPEVVAAVAPSTVKVSGIACNRLQEGSGFVT
                     VAPDVVVTNAHVVAGQTETEVELTDGSRLDAAVVVFDPDRDLAVLRVAGLSRNPLPIL
                     DAPEGASGGVFGYPGGAPLRVQPFQVGDEVRAVGTDIYDQRRTERAVLILASQLRPGD
                     SGGALVDGAGNVVGVAFAIAPDDPGVAYGLDVSELQAVLGGDLSTPVGTGSCLR"
     gene            complement(6070..6882)
                     /locus_tag="VLR27_15200"
     CDS             complement(6070..6882)
                     /locus_tag="VLR27_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HSP04855.1"
                     /translation="MTASPVTGPIPGDDPEKRFVEGDDVLLIDRKRRRYLVTLASGKE
                     FHSHAGVLAHDQLLGSVEGTTYRTTLGQWLLALRPTLNDIVLKMPRGAQVIYPKDIGP
                     IIIMADIHPGVRVLESGVGSGALSMGMLRAGAQITGYEVREDFAERAQANVERFAPAV
                     AANYDVQIRDCYEGIDEEHLDRVVLDLPEPWQVVPHAEKALRTGGILVAYTPTIGQAS
                     RLRDTLVESRFAFEETLEVLQRTWHIEGQSVRPDHRMVAHTGFLTSARLLAE"
     gene            complement(6879..>7690)
                     /locus_tag="VLR27_15205"
     CDS             complement(6879..>7690)
                     /locus_tag="VLR27_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP04856.1"
                     /translation="VLVAVSGGKDSLAVWDILLELGYQADGFVIGLGIGESSETSTAY
                     ARAFAVDRGLTLHETSLRDDHGFDVPTAAQVTRRVPCSACGLSKRHLFDKAAIDGGYD
                     AVATGHNLDDEAAVLFGNALRWNTEYLGRQRPVLPARDGFPRKVKPLVRLGEREMAAY
                     CVIRGIDYIVEECPMAAGNKHLRYKEALNAIEAESPGAKHAFYHEFLSKAADRFADDA
                     AGAIADGTSELGTCTRCGSPSSNEVCAFCLLVERTADAVPVTLGERRGARR"
BASE COUNT         1097 a         2720 c         2883 g          990 t
ORIGIN      
        1 acgttcttca gcgactcgac gccgtcgatc tcggcgagca gctgggggac gatggtggac
       61 tccatgtcgg agctgatgcc cgagccgcgg gtgcggaaca tcgagtccat ctcgtcgaag
      121 aacacgatga ccggccaacc ctcctcgctc ttctcccggg cccgctggaa caccaggcgg
      181 atctggcgct cggtctcgcc gacgtacttg ttcagcagct cggggccctt gatgttgagg
      241 aagaagctcc gggcctcgtc gccggtgctc tcggccacct tcttggccag gctgttggcc
      301 accgccttgg cgatgagggt cttgccgcag ccgggcgggc cgtagagcag gatccccttc
      361 ggcgccggca gctcgaactc ggcgaagagc tcctggtgca ggaacggcag ctcgaccgcg
      421 tcggcgatct gctcgatctg gtcgtcgagg ccgccgatgt cgtcgtagcc gacgtcgggc
      481 acctcctcga gcacgagctc ctcgacctcg ggccggggca gcttctcgag cagcaggccc
      541 gagcgggggt cgagcagcat ggtgtcgccg gaccggaggc gctcgcccac cagctcgtcg
      601 acaagctcca cgacccgctc ctcgtcggcc cggccgacca ccagcgcgcg gcggcggtcc
      661 tcgaggatct ccttgagcgt caccacctcg ccctgtccct cgggactgcg ggacaggatg
      721 atgctgaagc tctcgttgag cacgacctcg gcgccctggg gcagcgcgtc gacgtcgtcg
      781 gccagctcgg ggtgcaccgc cacccgcatc ttgcgcccac cggcgtggac gtcggcggtg
      841 ccgtcgtcgt tcatgcgcag gaacgtgccg taggcggagg ggggctgggt gagcttgtcg
      901 acctcctcgc gcagcgcggc gatgtgctcg cgcgcctcgc ggagggtgta gctcagcttc
      961 tcgttctggg acaccgcctg ggacagctgg cccttggtct cgagcaggcg ctcctcgagc
     1021 gtgcggaccc gcttcggcgc gtcctggagc cgctggcgca gcgcggcgac ctcggcctcg
     1081 agggcaacga gctgctcgcg cgagatgtcc tcgccgtgct cgccgtcgct tcctgtggtc
     1141 atggccggac tccctcttcg ggtgtgttcg cggaccatac accggtcctg cgggggtgcc
     1201 ccgcgcaccg ggcggcgaat tgtttgcgcg ggagttgccg ttcttattca tcccttcggt
     1261 agggtgcaga gcaccggcga tctcccccga tcgcttgacc atcgaaagcg caccgcagga
     1321 agggcgacac cgacatgaag gtctggatcg atcaggatct ctgcaccggc gacggtctgt
     1381 gcgaggagat cgcccccgat gtgttcgcac tcctcgacga cggcctcgcc tacgtcaagg
     1441 acggcgacaa gatcctcagc gagccgggcg gcgccgaagc cgtcgctccg gtgcccgccg
     1501 ggcaggaaga ggccgtcatc gagtcggccg aggagtgccc cggcgagtgc atcttcatcg
     1561 aggtgtgagg ctcgcctccg ggcgagcaca cgacaacgag tgactccggg cggcctcagg
     1621 gccgcccgtt gcgcgccccc gtcgggtcga aggcgcgcac cacgtcgagg aacgcctcga
     1681 tgtcggcacc ggtgatctcg atgctgaggg cgacgaaccc ccggcgggcc acgtagaagc
     1741 cggcctcgag gcagtggaag aagaacagct ccttgaggcg atcgtcggcg cctcggaggt
     1801 cctgggccct cgacaccggt ccgggcgtgc cgtgcacgtt catcagcgac ccggcgccgg
     1861 tcgcgcacat cggcagcccg gcggaggcga ggacctcgtt cagcgccgcc cgcatctcct
     1921 ccccgcgggc gtgcagatcg tcgagcaccg acccggtgag cagctcggac agcgcggcca
     1981 cgcccccggc catcgacgcc acgttgttgt tgaaggtgcc ggcgtgaccg agccggccgc
     2041 cctggccggc gtcgaagtgc gccatcacgt cggcccggcc gccgaacgcg ccgaacgtga
     2101 gcccgccggc gaggtacttg ccgagcgtgg tcaggtccgg ggtgatgccg aggagctgct
     2161 gcgcaccgcc ggcggaggac ctcgaggtca tgacctcgtc gaagaccagg agcgcacctg
     2221 cgtcccggca cagccggtgc agcgcctgga ggaagtcggg atccgccggg atgcagccgc
     2281 cggagccctg catcggctcg acgaggacgc aggcgatctc ggacccgtgc tcggcgaagg
     2341 ccgcctcggc gccgcccacg tcgttgtagt cgcacagcag ccactcgtgg ggcacgttca
     2401 ccgggtcgtc gtcccgcccg aaggtgagga cgccgccgtg gtagccgttg cggaagacga
     2461 cgatgcggcg acggccggtg tggtgggtgg cggcggccag ggccatcagg ttggcctcgg
     2521 tgccggagtt ggtgaaccgc acctgctcga gcgacgggaa ccgctcgacg agcagctcgg
     2581 ccaggcgcac ctcgtcctcg tgcacggcgc cgagggacca tcccgtcgac agcgcctcct
     2641 tcgccgcagc cagcaccggg gcgggcgagt ggccgaggag cccggcggtg tagttgccga
     2701 gcaggtcgac gtatcggtgc ccgtccacgt cccacaggtg cttgccggcg gcccgctcga
     2761 cccggaacgg gaacggctcg aactgcagga tcgaccgggt gttgccgccc ggcatcacct
     2821 tggccgcccg gtcggaccag ccggcgctga tcggtcgggc ttcggcgtac cgggctcgcg
     2881 cctcggccac tgcgacgtcg agatcggtcc ccgtcatggc gccaccgtac gacggttcgg
     2941 gatcagtcgg ccgtgacggc ggcggcgatg tggccgcact cccccgcgtc gcggtcgagg
     3001 accccgaccg cgctgctggc caggtcgagg cggaccgagc cgaggccgcg ccggcgctgg
     3061 aacagcgtcg agaccacggc tgcggactgg acgcgcgcgc tcggcacgat ggcggtccgc
     3121 cgggccaggg cccccgacct gctgtgctgg accccgccgt cctcggccca gcccaggccc
     3181 cggtaggcca gctcgccgag cagcggcccg aggacgagga cgatcgccgc cggcacgaac
     3241 caccagcgtg acgtcacgac tgccagaacg gagagggcgg aggcgccgag gagcgctccg
     3301 gcggcgccgc ggaccaggga ccgccggcgg gcgccggacg gcgccgggtg caggcggggg
     3361 cggggcacgg cggcgggcag gacccgggtg agcacccggt cgatctcggc ggcatcgagc
     3421 agcgggatgt ccacctggct ggcgtcgccc ccgccggagg ccgcgctccg gacccggagg
     3481 tcggcccggc cgaagggccg cagcagcagg cgttgggcca cgcgcgcgtc ctgcacgcgg
     3541 ctggtgcgga ccacgaactc gtgcttgcgg agcagcccgt aggtgacccg cagctcgtcg
     3601 ccgctgatgc tggcggtgag gtcccacttg cgcaccacgg cgatgagccc ggcgatcagg
     3661 aaccacaccg gcacgccgac gagcacgagc agggcgatgg ccgttcgccc gaggtccacg
     3721 cgtccgatct cgtcgggatc gatgccgagg tcggccagcg cgtcgacggc gaacacgagc
     3781 gacgcgagca cggccaccac ctcgggtccg gtgatcccgg ccaggaggag tcggccgggc
     3841 ggcaggcgga ccaggacctc ccggggggcg ggcggcggcg ggggcatcgg gccgtgccgt
     3901 gccgcgtccc agccctcccc gtccccacca gacccttcgg gcaccgcggc ccgcgcccgc
     3961 agggcgtcca gcacctggga gcgcagcgcc tcggcctcgt cccgcccgac cgcgtcgagg
     4021 cgcagctccg caccggaccc gccggcggtc tccacccgca ccgctgccaa cccgaagatc
     4081 tggtgccgga ggctgcgctc gagctccacg tgctggatgc ggtccaccgg gatccggcgc
     4141 tggttgcgga cgaacacccc ctcgtcgagg tggaggacgt cgccgtcgaa ggaccacagc
     4201 cggcggagcc agccgagcac gccagcgatg agggagatgg cgccggcgac cagcaggacg
     4261 agcacgaccc ggcccgagga gatggcgacg atcgccaccg gcaggaggtt ccgcacccgc
     4321 tcgggcgaga caacctcgaa ggccgccccg agcggcgagg tccgtcgcgg ccgtcggagg
     4381 tcgaccacgt cagaccgcgt cgccggtgcc gaccagcgcg aggatccggg cccgcagggc
     4441 ctcggcctcc gccgcgtcga tgccgggcac ggtcgagtcg ctcgacgcgg aggccgtgcg
     4501 caggatcagc gacgtcagcc ccatccgccg ctcgagcgga ccggcctcga ggtcgatgtg
     4561 ctggacccgg tggtacggga tggtcgactg ccggcgggtg agcacgccgt ggcgcaggtg
     4621 cagcgcgccg tcgccgaccc ggaactgcca ggcctgccac acgagcgacg tccaccagcg
     4681 cacgaggagc ccgccgacga cggcgagcac cagtgcggcg agggcacccg ggagggcctg
     4741 gcccaccccc gcgccggcag ccagcacgag cgccacccag acggccagcg cgatggtggc
     4801 gcgcagccgc cacaggctca ggacccggcg ctcgagcggg acgaggtcgc cgatgcccgg
     4861 gtcgtcggcg tcgggttcgg tcatcgcagg cacgacccgg tcccgaccgg ggtcgagagg
     4921 tccccgccca gcaccgcctg cagctcggag acgtcgaggc cgtacgcgac gccggggtcg
     4981 tcgggcgcga tggcgaaggc cacgccgacc acgttcccgg cgccgtcgac cagggccccg
     5041 ccggagtcgc ccggccgcag ctgggaggcc aggatcagca cggcccgctc ggtgcggcgc
     5101 tggtcgtaga tgtcggtgcc cacggcgcgg acctcgtcgc cgacctggaa cggctgcacc
     5161 cgcagcgggg cgccgcccgg gtagccgaag accccgcccg acgcgccctc gggcgcatcg
     5221 aggatgggca gcgggttgcg ggacagcccg gccacgcgga gcacggcgag gtcccggtcg
     5281 gggtcgaaga ccaccacggc cgcgtcgagg cgggagccgt cggtcagctc gacctcggtc
     5341 tcggtctggc cggccacgac gtgggcgttg gtcaccacga cgtccggggc gacggtgacg
     5401 aacccgctgc cctcctggag ccggttgcag gcgatgccgc tgaccttcac cgtcgacggc
     5461 gcgaccgcgg cgacgacctc gggggtgatc tgggcatcgg tgggcggtgg gccgacgtcg
     5521 ggcgccgggc gcatggcgtc gaacacgcgg gggaactggt cctcccccac caggcgccgg
     5581 agggcgacca gcgcgtccgg gggtgccggc aggatgcggt cgacgctgcg ggcgatcacc
     5641 gagttccggg cctgcgacga gaacgccccg gggacctcgg acatggtcgg caggagcagc
     5701 cagacggcga tcaccatgcc gagcacgccg gccaccgcac cgaaggcgtg gtcgacgatc
     5761 gtccactcgc tgttgcccag gttcacgcgc agccgcgatc cgaccacgac gccgaccgcc
     5821 tgaccgagga acgaccccac cagcagcacg atgccgccga gcaggaggag gctcgccggg
     5881 ctctcgtgct cgaactgctc gaccagccac ggcagcgtgc gcgcgccgag gaacaagccg
     5941 agtcccatcc ccacccacga ggcggtgcgg gtcaggaagc ccagccggta gccgcccacc
     6001 atggccagca cgccggtgcc gagcaggatg aggtcgagca ggttcacggc gcgggagggt
     6061 gccggggcgt cactcggcca ggaggcgggc gctggtcagg aagccggtgt gggcgaccat
     6121 ccggtggtcg ggccgcaccg actggccctc gatgtgccac gtgcgctgca gcacctcgag
     6181 ggtctcctcg aacgcgaagc gggactccac gagggtgtcg cgcaggcgcg acgcctgccc
     6241 gatggtgggc gtgtaggcga cgaggatccc gccggtgcgc agcgccttct cggcgtgggg
     6301 cacgacctgc cacggctcgg ggaggtcgag caccacccgg tcgaggtgct cctcgtcgat
     6361 gccctcgtag cagtcccgga tctggacgtc gtagttggcg gccaccgccg gggcgaagcg
     6421 ctcgacgttg gcctgggccc gctcggcgaa gtcctcgcgc acctcgtagc cggtgatctg
     6481 ggcgccggcg cggagcatgc ccatggacag cgcgcccgac ccgacgccgg attccagcac
     6541 gcggaccccg gggtggatgt cggccatgat gatgatcggg ccgatgtcct tggggtagat
     6601 cacctgggcg ccgcggggca tcttgagcac gatgtcgttc agcgtgggac gcagcgccag
     6661 cagccactgc ccgagcgtgg tgcggtaggt ggtgccctcc acggagccga gcagctggtc
     6721 gtgggccagc acgccggcgt ggctgtggaa ctccttgccg gacgcgaggg tgacgaggta
     6781 gcggcgtcgc ttgcggtcga tgagcaggac gtcgtcgccc tcgacgaagc gcttctcggg
     6841 gtcgtcgccg gggatggggc cggtgaccgg cgaggcggtc atcgtcgggc tccgcggcgc
     6901 tcgccgaggg tgaccggcac ggcgtcggcg gtgcgctcga cgagcaggca gaaggcgcag
     6961 acctcgttcg acgacggcga cccgcagcgg gtgcacgtgc ccagctcgga cgtgccgtcg
     7021 gcgatcgcgc cggcggcgtc gtcggcgaag cggtcggccg ccttgctgag gaactcgtgg
     7081 tagaaggcgt gcttcgcgcc gggcgactcg gcctcgatgg cgttcagggc ctccttgtac
     7141 cgcaggtgct tgttgccggc ggccatgggg cactcctcga cgatgtagtc gatgccacgg
     7201 atgacgcagt acgccgccat ctcgcgctca ccgagtcgga cgagcggctt gaccttgcgg
     7261 gggaagccgt cgcgggcggg caggacgggc cgctggcgac ccaggtactc ggtgttccag
     7321 cgcagggcgt tcccgaacag cacggccgcc tcgtcgtcga ggttgtggcc ggtggccacg
     7381 gcgtcgtagc caccgtcgat ggccgccttg tcgaacaggt gccgcttgct caggccgcac
     7441 gccgagcacg ggacgcggcg ggtgacctgg gcggcggtgg gcacgtcgaa gccgtggtcg
     7501 tcgcgcagcg aggtctcgtg gagcgtcagg ccgcggtcga cggcgaaggc ccgggcgtag
     7561 gcggtcgagg tctcggagga ctcgccgatg ccgagaccga tgacgaagcc gtcggcctgg
     7621 tagccgagct cgaggaggat gtcccagacg gcgagggagt ccttcccacc ggagaccgcc
     7681 accagcaccc
//
LOCUS       DATISU010000387         3181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_497755, whole genome shotgun sequence.
ACCESSION   DATISU010000387 DATISU010000000
VERSION     DATISU010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497755"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /gene="rplW"
                     /locus_tag="VLR27_15210"
     CDS             complement(<1..136)
                     /gene="rplW"
                     /locus_tag="VLR27_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HSP04857.1"
                     /translation="MDARSVIIRPVVSEKSYDLIEGNVYTFVVHPDANKTQIRQAIEQ
                     I"
     gene            complement(136..774)
                     /gene="rplD"
                     /locus_tag="VLR27_15215"
     CDS             complement(136..774)
                     /gene="rplD"
                     /locus_tag="VLR27_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HSP04858.1"
                     /translation="MPSIDVRNISGDTVGSADLDEAIFGIEPNVAVMHQVVTAQLAAK
                     RSGTQSTKTRAEVRGGGAKPWKQKGTGRARQGSIRAPQWRGGGVALGPKPRSYAQKTP
                     KKMIKLALRSALSDRFASERIIVLDSWGLDAPKTKTVRVALETLGTSGRVVAVVSRDD
                     VNTNKSLANLPNVHVVQPGELNTYDVLVSDWLVFTTDTLPSSAPAASEEGAA"
     gene            complement(774..1418)
                     /gene="rplC"
                     /locus_tag="VLR27_15220"
     CDS             complement(774..1418)
                     /gene="rplC"
                     /locus_tag="VLR27_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HSP04859.1"
                     /translation="MATKSIVATKVGMTQVWNEEGHIVPVTMLRVQPCRVVQIKTQER
                     DGYSALQVTYGQRDARKLTMPKAGHFAAAGVDPGETLVELRLDDVSAYEVGQELAADV
                     FAAGDLVDITGVSKGKGFAGVMKRHGFGGMPASHGSHRVHRKPGAIGACATPARVFKG
                     HRMAGRMGNERVTTQNLVVVESDPEREIVLIRGSVPGPKQSTVLIKDASKKAVS"
     gene            1776..1937
                     /locus_tag="VLR27_15225"
     CDS             1776..1937
                     /locus_tag="VLR27_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04860.1"
                     /translation="MPEHLHLEGTTLDHPAWMADQRDDIFDAAALERAMVESRQAAYD
                     ALRELAPAS"
     gene            2015..2749
                     /locus_tag="VLR27_15230"
     CDS             2015..2749
                     /locus_tag="VLR27_15230"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HSP04861.1"
                     /translation="MRLYALAVPEPDAELVADRCWQAGAAGIWESAAEGGSVTLRVGV
                     EEADVDAFEAALATHGPVDVTATDLVELSTRTVELPAESGRVRIEVPPTVFGDGLHPT
                     TASCLELVADLAGPGTTLLDVGCGSGALSVVAARAGAVVTAIDIDPVAVEATIANAAA
                     NEVDVDATTTPLTDLSGTWDVVMANISARAVLELSDDLWRVCAGTLVVSGILAERWSS
                     VRERLGGTVLRVHDVDGWVTASVTRS"
     gene            complement(2848..3168)
                     /gene="rpsJ"
                     /locus_tag="VLR27_15235"
     CDS             complement(2848..3168)
                     /gene="rpsJ"
                     /locus_tag="VLR27_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HSP04862.1"
                     /translation="MADSQKIRIRLKAYDHEVIDTSTKKIVETVLRTQAVVRGPIPLP
                     TEKHRFTVVRGPFKDKDSREHFEMRIHKRLIDILEPSAKTVDSLQRIELPAGVDIEIK
                     IQQA"
BASE COUNT          447 a         1045 c         1155 g          534 t
ORIGIN      
        1 agatctgctc gatcgcctgg cggatctggg tcttgttggc gtccgggtgg acgacgaagg
       61 tgtagacgtt gccctcgatg aggtcgtagc tcttctcgga gacgaccggg cggatgatga
      121 cggatcgtgc gtccatcagg cagcaccttc ctcggaggcg gccggagccg acgacgggag
      181 ggtgtcggtg gtgaagacca accagtcgct gaccagcacg tcgtaggtgt tgagctcgcc
      241 cggctggacc acgtgcacgt tcggcaggtt ggccaggctc ttgttcgtgt tgacgtcgtc
      301 tcgggagacg acggccacca cccgaccgct ggtgcccagg gtctcgaggg ccacgcggac
      361 ggtcttggtc ttgggagcgt cgaggcccca gctgtcgagg acgatgatgc gctcggaggc
      421 gaagcggtcc gagagggccg atcgcagggc gagcttgatc atcttcttcg gggtcttctg
      481 ggcgtagctg cggggcttgg ggccgagggc cacaccgccg ccgcgccact ggggcgcacg
      541 gatggagccc tggcgagccc gaccggtgcc cttctgcttc cacggcttgg caccaccgcc
      601 gcgcacctcg gcgcgggtct tggtggactg ggtgccggag cgcttggcgg cgagctgggc
      661 ggtgacgacc tggtgcatga cggcgacgtt cggctcgatg ccgaagatcg cctcgtccag
      721 gtcggcgctg ccgaccgtgt caccggagat gttgcgcacg tcgatggagg gcatcagctg
      781 accgccttct tgctggcgtc cttgatgagc acggtcgact gcttggggcc ggggaccgat
      841 ccgcggatga gcacgatctc gcgctcggga tcggactcga ccacgaccag gttctgggtg
      901 gtgacccgct cgttgcccat gcggccggcc atgcggtggc ccttgaacac acgagccggc
      961 gtggcgcagg cgccgatggc gcccggcttg cggtgcaccc ggtgcgagcc gtggctggcg
     1021 ggcatgccgc cgaagccgtg gcgcttcatg acgcctgcga agcccttgcc cttggagacg
     1081 ccggtgatgt cgaccaggtc ccccgcagcg aacacgtccg cggcgagctc ctgaccgacc
     1141 tcgtaggcac tgacgtcgtc gaggcggagc tcgacgagcg tctccccggg atcgacgcca
     1201 gcagccgcga agtgaccggc cttgggcatg gtgagcttgc gggcatcacg ctggccatag
     1261 gtgacctgga gtgcgctgta accgtctcgc tcttgtgtct tgatctggac cacccggcag
     1321 ggctgcacgc ggagcatggt gacggggacg atgtgtccct cctcgttcca tacctgcgtc
     1381 attcccactt tggtggcgac gatcgatttc gtggccatgc ggcgcgtcct cgttcacgcg
     1441 aacgctccgc agggcacgta ctggcccagc ggagcgctgg ttcggttgtg ccgtgcggtt
     1501 cccgatccgg atgtcggacc aggcctgcac gggcctcgat tgcagaaagc tgcggcctcc
     1561 ggacaggggt ccgggggcac gaagaggaaa cgatagcggg gcagcacgcc gaacaccaat
     1621 gcgccgcaga tcgcacctcc aggcaaccac ggcgcgccga gccgggcgcg gatgggcggg
     1681 acgtgacgtc ggggcccggc gggtgtcacg acaccgacac agagcgtcat gtggtcgaca
     1741 ccggacatcc atgtccatca ggtgcaatgg cccccatgcc agagcacctg cacctcgagg
     1801 gcaccacgct ggaccatccg gcctggatgg ccgaccagcg agacgacatc ttcgacgccg
     1861 cggcgctcga acgggcgatg gtcgagtccc gccaggcggc ctacgacgcg ctgcgggagc
     1921 tcgctccggc gtcctgaccc cgccccacca cggacagctc tgaccgtgca ccgcccgagt
     1981 ggcggtgcac ggccgcgttc ggggagggtt gggcgtgcgc ctctacgccc tcgccgtgcc
     2041 cgagcccgac gccgagctgg tcgccgaccg gtgctggcag gccggcgccg ccgggatctg
     2101 ggagtccgcc gctgaaggcg gctccgtcac gctgcgcgtc ggggtcgagg aggccgacgt
     2161 cgacgcgttc gaggcggccc tcgccaccca cggtccggtg gacgtgaccg ccaccgacct
     2221 ggtcgagctg tccacccgga ccgtcgagct ccccgccgag agcggtcggg tgcgcatcga
     2281 ggtgccgccg accgtgttcg gcgacggcct gcaccccacg acggccagct gcctcgagct
     2341 ggtcgccgac ctcgcgggac cggggaccac gctcctggac gtgggctgcg ggtcgggggc
     2401 gctgtcggtg gtggcggcgc gcgccggagc cgtggtcacc gcgatcgaca tcgacccggt
     2461 ggccgtcgag gccacgatcg ccaacgccgc ggccaacgag gtggacgtgg atgccaccac
     2521 cacgcccctg acggacctca gcgggacgtg ggacgtcgtc atggccaaca tctccgcccg
     2581 ggccgtgctg gagctctccg atgacctgtg gcgggtctgc gccgggacgc tggtggtctc
     2641 gggcatcctc gctgagcgct ggtcctcggt ccgggagcgt ctcggtggga ccgtgctccg
     2701 cgtgcacgac gtcgatggtt gggtcactgc ttctgtgacc cggtcctagg aaactggcag
     2761 tcacctcgtc acacgcgcag aacgccgccc cggcgtggga cggcgttccg tgtgacgatc
     2821 tgactgccag tttcagtttc ggcagtctta cgcctgctgg atcttgatct cgatgtcgac
     2881 cccggcgggg agctcgatgc gctgcagcga gtccacggtc ttggcggacg gctcgaggat
     2941 gtcgatcagg cgcttgtgga tccgcatctc gaagtgctcg cggctgtcct tgtccttgaa
     3001 cggaccacgg acgacggtga agcggtgctt ctcggtgggg agcgggatcg ggccccggac
     3061 gacggcctgc gtgcgcagga cggtctcgac gatcttcttc gtcgaggtgt cgatgacctc
     3121 gtggtcgtac gccttcaggc ggatgcggat cttctgactg tcagccatga cggtgacctg
     3181 g
//
LOCUS       DATISU010000388         7172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_405048, whole genome shotgun sequence.
ACCESSION   DATISU010000388 DATISU010000000
VERSION     DATISU010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405048"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VLR27_15240"
     CDS             complement(<1..180)
                     /locus_tag="VLR27_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04863.1"
                     /translation="MPSERLRSSVRAGGRGLVASMAMTGMRELTVGMGWLERTPPREI
                     LRDQAPLLVLPLSDGH"
     gene            277..1137
                     /locus_tag="VLR27_15245"
     CDS             277..1137
                     /locus_tag="VLR27_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04864.1"
                     /translation="MPIDPDEPGGSHDPTRANDPIDPTVAQDPVDATHVQGAAPAAGM
                     RPSERRFDPLDEPAPRHPDDDRDRTGAWWPWALLAAVLIGIVLFALLGGDDDDDDVGT
                     TDQTTDTLAPETSVEDTVTEDTTAETTAPDSTEDTAATEDTTATSAPATGGDAPATDA
                     GDDPGTVTTTDGTDLFTLVQGDAGDAGDAERLSPHADQDVTGEGVYVLEVVDGEGFWI
                     GASDQQRIFAHAPDDVSVEAGQRIGFDGFLEANPAEDSADVHDIPEDQGAELHRQQGH
                     HIELRSLTPA"
     gene            1181..1828
                     /locus_tag="VLR27_15250"
     CDS             1181..1828
                     /locus_tag="VLR27_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04865.1"
                     /translation="MADDAGDEAAESERAPSRRRTGRALAVVLALVALGAVVAAVSLW
                     TSRADLQDDVDRRAEIASVAGRFAEALLSYDHEDLDASRSEVRDLATSEFADEYVDAF
                     DAGLGGQIESLEATSSATIREVFVSSSTGDTARAVVIADTEIESDAGSRATVGTYLDI
                     SLVRLDGRWQVDDVSSVANAGSRLAPVPGSDVDEGTADDPDADGTDSTSTTTSAP"
     gene            complement(1836..3191)
                     /locus_tag="VLR27_15255"
     CDS             complement(1836..3191)
                     /locus_tag="VLR27_15255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HSP04866.1"
                     /translation="MNRRVLLNLAAFGLVSAVVIVWSIATLFEVDALDDPDFVTVEFE
                     SSPGLARGFEVAYLGHAVGSVHSAALRDGYSEIVLRIDKDEGLPAAVDALARRKSAIG
                     EPFVDLMPTPGTDPTSGPRLGDGDRIPLERTSSPISYGTLFKSVDALLAAIDPDQVGT
                     VVDELAAALDGRGDDLRRIIAGSRALTERTVANGDEIDQFLADLGDITGVLADNRDPL
                     ARSIDAFDAVTQTLDETRGPIEQLLQEGPSALQLVLRIMASTDAQLVCTVDGLAALGP
                     VTADGVAEALGATIADSGTALDITNAVIGADGYLNLTLLFSDTNEAPVYPERRPPPVA
                     PSVDGCGDLTGRTVGAELGSRADLGPGGRDDLAADGSVDPDERAIDPDDGGPTGSSDL
                     DEPDEPLLAEVIRKALPLLLLAALVALAWWLWKRRQEAGEGPGDEPEALPDDADETVG
                     S"
     gene            complement(3188..4162)
                     /locus_tag="VLR27_15260"
     CDS             complement(3188..4162)
                     /locus_tag="VLR27_15260"
                     /inference="COORDINATES: protein motif:HMM:NF023315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HSP04867.1"
                     /translation="MSRAALARLAVLVVLTLLAGACTLQTAGGPKGDRTFVARFDDIQ
                     HLVVGHSVRVADVTVGTVTSLRLDGYDAVVEMSIVDGRELPVGTVASVSATSLLGENY
                     VRLRLPDEVEPPYHEDGDELPTSSADASFEELTIQLLGVLRAIEGRDVADIVDSGATA
                     IGGRGDELGGLLQSLDELGDSLVGQSEQLVTALDSFGRLGGDLAAGADRFGDSIERTA
                     EATGSLAAQGDRMVVLLEELTRVAGTLDANVLAPHREELDRILDRLAPIADVLVEDVD
                     TLIALLEALRTASPLIPRMIEDHEALTYGIFDTFVVPGQEPIHVPEGP"
     gene            complement(4159..5337)
                     /locus_tag="VLR27_15265"
     CDS             complement(4159..5337)
                     /locus_tag="VLR27_15265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HSP04868.1"
                     /translation="MRPPRRRLGLTGAVLAVVLAAFALPSCGGGGASGYEVTASFARA
                     VALYEGADVLVMGIDVGDVTAIEIVGGGIDVTMSIDEVVPLPADATAAIVPSSLIGER
                     TVVLGPAWRDGDDELADGDRIPVERTIIPVEPDEALQSVTELLQSLDPNSVRRLLAEG
                     AGALEGNGRTLNQALGELAQLIPYLAEQDDELLELAGDVNVLADVIRSRDTEVGALLT
                     DFAAVSSALAEERESLARFLTSLTSLTLQGEELLTAYETSLPEDMDTLAAVALTVKAN
                     GGSVRQLLLSLRGFQQGVIEAYDPANRSIRARVYTSQTLLNPVMELLLELGLVPDSDP
                     IPLPDPDDLPLPIPVPDPGDLPTPTIPTPTIPPPTVPDPGDDDGGTLCVPLLDPGCDG
                     "
     gene            complement(5343..6359)
                     /locus_tag="VLR27_15270"
     CDS             complement(5343..6359)
                     /locus_tag="VLR27_15270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HSP04869.1"
                     /translation="MSRFHLRSFADRSPLVLGILTLVAAFIAVVLAFAIGTLGILEDR
                     YEMSGVFEESGGIRPGDKVRVAGLDVGEVTSVRPDFQEAQVVVTWEVDRGVDLGADTS
                     AEISLATLLGGRYLRLDGPVTEPYVADLPTEERRIPIERTRLPIGVQDALGRFTDAAD
                     AYDVDQIDQLLQNFADISADNADSFEPLVENITVLASVLNERRGQIDSLLQNTTELTS
                     ALASKDQELLTLIDQSGRLLDVVAQRRDQLRAVLGSGSQAVSELDRLITANRESLDTL
                     LADFDITADTLRARLPELNELLAYVGPAVQGLDEATRSGPWLDSIVSGTGLVQVPDLI
                     ESGG"
     gene            complement(6356..>7172)
                     /locus_tag="VLR27_15275"
     CDS             complement(6356..>7172)
                     /locus_tag="VLR27_15275"
                     /inference="COORDINATES: protein motif:HMM:NF023315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HSP04870.1"
                     /translation="VEVRMGKAWVEFDLDETVELPRDSEVAVRAQNLLNVRELVLTPG
                     EAGELLADGDVMENTSSSVELGNLINELGPLLEAVDPEQVNTLVTALNEALGGNRERV
                     AGLTSDFEVVVGNLAARSDTIGQVIDDYAVISEEVARRDRQIQQLVDNLVLLTGTYDE
                     SEQTLIDALATLPGFSDRLAGLLSENAGNLDSILADLAALGTVTRQNRDLVDDVVRLA
                     PPALLELYTITDRADALVNNYLCTAAHPPPCPHPTTDQPGSSTSDVFEELLRP"
BASE COUNT         1015 a         2513 c         2689 g          955 t
ORIGIN      
        1 gtgcccgtcg gacaggggca ggaccagcag cggcgcctgg tcgcgcagga tctcccgcgg
       61 cggcgtgcgc tccagccagc ccatgccgac ggtgagctcg cgcatgccgg tcatggccat
      121 cgacgcgacg aggcctcggc ccccggcgcg cacggaggag cggagtcgtt cgctgggcat
      181 cgccgtcccc tacccgatcc gccgtcccga ggttcaagac gttgagcgcc acgggtagtg
      241 aaccggcctg ctccccccag gaccggaggt cccgtcatgc cgatcgatcc cgacgagcca
      301 ggcgggtcgc acgacccgac ccgggcgaac gacccgatcg acccgaccgt cgcccaggat
      361 ccggtcgacg ccacccacgt gcagggcgct gcgccggccg ccgggatgcg cccgtccgag
      421 cgccggttcg atccgctgga cgagcccgcc ccccgccatc ccgacgacga ccgcgaccgc
      481 accggtgcat ggtggccgtg ggcgctgctg gccgcggtcc tgatcggcat cgtgctcttc
      541 gccctgctcg gcggggacga cgacgatgac gacgtcggca cgaccgacca gacgaccgac
      601 acgctcgcgc cggagacctc ggtggaggac accgtcaccg aggacacgac cgccgagacg
      661 accgcgccgg acagcaccga ggacacggcg gcgaccgagg acacgacggc cacgagcgca
      721 ccggcgaccg gcggcgacgc cccggcgacc gatgccggcg acgacccggg cacggtcacc
      781 accaccgacg gcaccgacct gttcaccctc gtccagggcg acgccggcga cgccggcgac
      841 gccgagcgcc tctcgcccca cgccgaccag gacgtgaccg gcgagggcgt ctacgtgctg
      901 gaggtcgtcg acggcgaggg cttctggatc ggggccagcg accagcagcg catcttcgcc
      961 cacgctcccg acgacgtctc ggtcgaggcc ggccagcgca tcggcttcga cgggttcctc
     1021 gaggccaacc cggccgagga ctccgccgac gtgcacgaca tccccgagga ccagggggcc
     1081 gagctgcacc gccagcaggg ccaccacatc gagctccgct cgctgacccc ggcctgagcg
     1141 acggcgcagg gcgtccggcg cgcaccgata cgatccggcc gtggccgacg acgctgggga
     1201 cgaagccgcc gagtccgagc gcgcgccgtc gaggcgacgc acgggccggg cgctggcggt
     1261 ggtgctggcg ctggtcgccc tgggagcggt cgtcgcggct gtctcgctgt ggacgtcccg
     1321 ggccgacctc caggacgacg tcgatcgccg ggccgagatc gcctcggtcg ccgggcgctt
     1381 cgccgaggcg ctcctctcct acgaccacga ggacctcgat gcctcgcgga gcgaggtgcg
     1441 cgacctcgcc acgtccgagt tcgccgacga gtacgtggac gccttcgatg ccgggctcgg
     1501 cggccagatc gagagcctgg aggccacctc ctcggcgacg atccgcgaag tgttcgtgtc
     1561 gtcgtccacc ggcgacaccg cccgcgccgt cgtcatcgcc gacaccgaga tcgagagcga
     1621 tgccggcagt cgggccaccg tcggcaccta cctcgacatc agcctcgtcc gtctcgacgg
     1681 ccgctggcag gtcgacgacg tgagctcggt ggccaacgcc ggctcccgcc tcgcgccggt
     1741 gcccggctcc gacgtcgacg aggggacggc cgacgatccg gacgccgacg gcacggactc
     1801 cacatcgacc accacgtcgg ccccctgagc cggggtcagc tcccgacggt ctcgtcggcg
     1861 tcgtccggga gggcctcggg ttcgtcgccc ggaccctcgc cggcctcctg gcgccgtttc
     1921 cagagccacc aggcgagcgc gacgagcgcc gccagcagca ggagcggcag cgccttccgg
     1981 atgacctcgg ccaagagcgg ctcgtcgggc tcgtcgaggt cggaggaccc cgtcgggcca
     2041 ccatcgtccg gatcgatcgc acgctcgtcg gggtcgaccg agccgtcggc ggccaggtcg
     2101 tcacgaccgc ctggcccgag gtcggcgcgg ctgcccagct cggcgccgac ggtgcgaccg
     2161 gtgaggtcac cgcagccgtc gacggacgga gccacgggcg ggggccggcg ctcggggtag
     2221 accggcgcct cgttggtgtc ggagaacagc agcgtcaggt tcagatagcc gtcggcgccg
     2281 atgacggcat tcgtgatgtc gagggcggtc ccggagtcgg cgatggtggc cccgagggcc
     2341 tcggcgacgc cgtcggcagt caccgggccg agcgcggcga ggccgtcgac cgtgcacacc
     2401 agctgcgcgt cggtcgaggc catgatccgg agcaccagct gcagcgcgga cggtccctcc
     2461 tggagcagct gctcgatggg tccacgggtc tcgtccagcg tctgggtgac ggcgtcgaag
     2521 gcgtcgatgc tccgggcgag cgggtcccgg ttgtcggcga gcacacccgt gatgtcgccg
     2581 agatcggcga ggaactggtc gatctcgtcg ccgttggcca cggtgcgctc cgtcagcgct
     2641 cgggacccag cgatgatccg acgcaggtcg tccccccggc cgtcgagcgc ggcggccagc
     2701 tcgtcgacca cggtgccgac ctgatccgga tcgatggccg ccagcagcgc atcgacgctc
     2761 ttgaagagcg tgccgtagct gatcgggctc gacgtccgct ccagcgggat ccggtcgccg
     2821 tcgccgagtc ggggtccgct cgtcgggtcg gtgccgggcg tcggcatcag gtcgacgaag
     2881 ggctcgccga tcgccgactt gcgacgggcg agggcatcga ccgcggcggg cagcccctcg
     2941 tccttgtcga tgcgcaggac gatctcggag tacccgtctc gcaatgcggc cgagtgcacg
     3001 gagccgaccg cgtggccgag gtacgcgacc tcgaaccccc gagcgaggcc gggcgaggac
     3061 tcgaactcga cggtcacgaa gtccgggtcg tcgagcgcgt cgacctcgaa gagggtggcg
     3121 atggaccaca cgatcaccac cgcgctcacg agcccgaacg cggcgaggtt gaggaggacc
     3181 cggcggttca cggtccctcc ggcacgtgga tcggctcctg gcccgggacc acgaaggtgt
     3241 cgaagatccc gtacgtcagg gcttcgtggt cctcgatcat ccgggggatc agcgggctcg
     3301 ccgtgcggag cgcctcgagc agggcgatga gcgtgtcgac gtcctccacg aggacgtcgg
     3361 cgatcggcgc cagccggtcg aggatccggt ccagctcctc gcggtgcgga gcgagcacgt
     3421 tggcgtccag cgtgccggcg acgcgagtga gctcctcgag cagcaccacc atgcggtcgc
     3481 cctgcgcggc gaggctgccg gtggcctcgg cggtccgctc gatcgagtcg ccgaagcggt
     3541 cggcgcccgc cgcgaggtcg ccgccgaggc ggccgaagga gtcgagggcg gtcaccagct
     3601 gctcggactg gcccaccagg ctgtcgccca gctcgtcgag gctctggagc agcccgccga
     3661 gctcgtcacc gcggccaccg atcgcggtgg caccggagtc gacgatgtcg gcgacgtcgc
     3721 gcccctcgat ggcccgcagg acaccgagca gctggatggt gagctcctcg aagctggcgt
     3781 cggccgagct cgtgggcagc tcgtcgccgt cctcgtggta cggcggctcg acctcgtcgg
     3841 ggaggcgcag ccgcacgtag ttctcgccga gcagcgaggt ggccgacacc gacgccacgg
     3901 tgccgacggg gagctcccgg ccgtcgacga tcgacatctc gaccacggcg tcgtagccat
     3961 cgaggcggag gctggtgacc gtgccgaccg tgacgtcggc gacccgcacc gagtggccga
     4021 ccacgaggtg ctggatgtcg tcgaagcggg ccacgaacgt gcggtcgccc ttgggaccgc
     4081 cggcggtctg cagcgtgcac gccccggcga gcagcgtgag cacgaccagg accgcgaggc
     4141 gggcgagcgc ggcgcggctc atccgtcgca tcccgggtcg aggagcggga cgcagagcgt
     4201 ccccccgtcg tcgtcgccgg ggtcggggac ggtcggtggc gggatggtcg gcgtggggat
     4261 ggtgggggtc ggcaggtcac cggggtccgg caccgggatg ggcagtggca ggtcgtcggg
     4321 gtccggcagc gggatcgggt ccgagtcggg cacgaggccg agctccaaca ggagctccat
     4381 gaccgggttg agcagggtct gcgacgtgta cacccgggcc cggatcgagc ggttcgccgg
     4441 gtcgtaggcc tcgatgacgc cctgctggaa gcctcgcagg gacaggagga gctggcgcac
     4501 cgagcccccg ttggccttca cggtgagggc gacggcggcg agggtgtcca tgtcctcggg
     4561 cagcgacgtc tcgtaggcgg tgagcagctc ctcaccctgc agggtcagcg acgtcagcga
     4621 cgtcaggaac cgggccaggg actcccgctc ctcggcgagc gcgctggaca cggcggcgaa
     4681 gtcggtgagc agcgcaccga cctcggtgtc gcgcgagcgg atcacgtcgg cgaggacgtt
     4741 gacgtcgccg gcgagctcaa gcagctcgtc gtcctgctcg gccaggtacg ggatgagctg
     4801 ggccagctcg ccgagcgcct ggttcagggt ccggccgttg ccctcgagcg ccccggcccc
     4861 ctcggccagc aaccggcgga ccgagttcgg gtcgagcgac tggagcagct cggtgaccga
     4921 ctgcagcgcc tcgtcgggct ccaccgggat gatcgtccgc tcgaccggga tgcggtcgcc
     4981 gtcggcgagc tcgtcgtcgc cgtcgcgcca ggccggtccg agcacgacgg tgcgctcgcc
     5041 gatgagcgag ctcgggacga tggccgcggt ggcgtcggcg ggcagcggca cgacctcgtc
     5101 gatggacatg gtcacgtcga tcccaccgcc gacgatctcg atcgcggtga cgtcgccgac
     5161 gtcgatcccc atgacgagca cgtccgcgcc ctcgtagagg gcgacggcac gggcgaacga
     5221 cgccgtcacc tcgtagccgc tggcgccgcc cccgccgcag gagggaaggg cgaacgcagc
     5281 cagcaccacg gcgaggaccg cgccggtgag gccgagacgg cgacggggag gtcgcacggc
     5341 gatcatccgc cgctctcgat caggtcgggc acctggacga gcccggtgcc cgacacgatg
     5401 gaatcgagcc acggccccga gcgggtggcc tcgtcgaggc cctgcaccgc aggccccacg
     5461 taggcgagca gctcgttgag ctcgggcagg cgggcgcgga gggtgtcggc ggtgatgtcg
     5521 aagtcggcga ggagcgtgtc caggctctcg cggttcgcag tgatcaggcg gtcgagctcg
     5581 gacaccgcct ggctccccga gccgagcacg gcgcgcagct ggtcccgccg ctgggccacc
     5641 acgtcgagca gccggccgga ctggtcgatc agcgtcagca gctcctggtc cttgctggcg
     5701 agggccgagg tcagctcggt ggtgttctgc aggagcgaat cgatctgccc ccgtcgctcg
     5761 ttgagcacgc tcgccaggac cgtgatgttc tcgacgaggg gctcgaagga gtcggcgttg
     5821 tcggcggaga tgtcggcgaa gttctggagg agctggtcga tctggtcgac gtcgtaggcg
     5881 tcggcggcat cggtgaagcg cccgagcgcg tcctgcacgc cgatcggcag acgggtgcgc
     5941 tcgatgggga tgcggcgctc ctcggtcggg aggtcggcga cgtagggctc ggtgaccggc
     6001 ccgtcgaggc ggaggtagcg cccgccgagc agcgtggcca ggctgatctc ggccgacgtg
     6061 tccgcgccga ggtcgacgcc gcgatcgacc tcccacgtca cgacgacctg tgcctcttgg
     6121 aagtcgggcc gcaccgaggt gacctcgccg acgtcgaggc cggccacgcg caccttgtcg
     6181 ccggggcgga tgccgccgct ctcctcgaag acgccgctca tctcgtagcg gtcctcgagg
     6241 atcccgagcg tgccgatggc gaaggcgagc accacggcga tgaaggcggc gaccagcgtg
     6301 aggatgccga gcaccagggg gctgcggtcg gcgaagctgc ggaggtggaa gcggctcatg
     6361 gtcggagcag ctcctcgaag acgtcggacg tcgacgaccc gggctgatcc gtggtcgggt
     6421 gggggcacgg cggcgggtgc gccgccgtgc agaggtagtt gttgacgagg gcgtcggccc
     6481 ggtcggtgat cgtgtagagc tcgagcagcg ccggcggggc gagccggacg acgtcgtcga
     6541 ccaggtcgcg gttctgccgg gtcaccgtgc cgagggcggc gaggtcggcg aggatgctgt
     6601 cgaggttgcc agcgttctcc gacagcagcc cggccaggcg gtcgctgaag ccgggcaacg
     6661 tggccagcgc gtcgatcagc gtctgctcgc tctcgtcgta ggtgccggtg agcaggacca
     6721 ggttgtcgac gagctgctgg atctggcggt cgcgccgggc cacctcctcg ctgatgacgg
     6781 cgtagtcgtc gatgacctgg ccgatggtgt cgctgcgggc ggcgaggttg ccgaccacga
     6841 cctcgaagtc gctggtgagt ccggccaccc gctcccggtt gccgccgagc gcttcgttca
     6901 gggcggtgac gagggtgttg acctgctcgg gatcgacggc ctccagcagg gggccgagct
     6961 cgttgatgag gttgcccagc tcgaccgacg acgaggtgtt ctccatcacg tcgccgtccg
     7021 ccagcagctc gcccgcctcg cccggggtga ggaccagctc gcgcacgttg agcaggttct
     7081 gggcccgcac cgccacctcg ctgtcgcggg ggagctccac cgtctcgtcg aggtcgaact
     7141 ccacccaggc cttgcccatg cgcacctcga cc
//
LOCUS       DATISU010000389        31239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_871899, whole genome shotgun sequence.
ACCESSION   DATISU010000389 DATISU010000000
VERSION     DATISU010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 31239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31239
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871899"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VLR27_15280"
     CDS             <1..407
                     /locus_tag="VLR27_15280"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSP04871.1"
                     /translation="AWDALARVDAYRRWWPWLRRFDAERLASGERWEARIRIPMPWSL
                     RFTLDLRDVEAPVRVIADVTGDIEGTATVTIERGGDGAEIRLRSDLAPRHRLLRGVNR
                     ILPGVSRRLHDRVVDRAFQQFADRDRSGAVER"
     gene            556..3306
                     /locus_tag="VLR27_15285"
     CDS             556..3306
                     /locus_tag="VLR27_15285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HSP04872.1"
                     /translation="MSSTPATAPTREVAATFHDRTVDEVLETLSVDPGAGLTTDEVVR
                     RREEHGSNELDEAPRDPSWRRFARQFKDLLILILLAAAGISFVVSGELKTPIVVLVVV
                     LLNAVIGFVQENRAEASLDALRRMLVLEVRVRRDGTMRTVPAAELVPGDVVAVEAGDR
                     IPADGRVIEAVQLEVEEAALTGESLPVAKVPDPVPGEDVPVADRTSMAHMQTTVTRGR
                     GELVITTTGMGTEIGRIAGLLREAAPEKTPLQRQLDHLAHSLAKLAGVIVGLVFLIGL
                     IRGEDISDLLLTAVALAVASIPEGLPAVTAVTLAIGVSKMARENAIVKRLASVETLGS
                     TTVICSDKTGTLTLNQMTAEEIVVTGRRLAISGNGYAPEGEIQGLDDVPIDLDEVLEP
                     MALCSDATVRFDEEEGVWRLVGDPTEGALVVLAGKGGVDPATRRAERPRVGEVPFDSA
                     HKLMATLHDSESDLRMYAKGAPDAILDRSTTVLGYDGTIVPIEEAREELEEHNARLAS
                     SGMRVLAVARRVISRDEWKGDGDLDAVTDLTFLALIGIVDPPREEARLAIEEAGGAGI
                     SVKMITGDHASTAKAIGEQLSLGGGDQVVAVTGRDLDAMSDDELAERIEDISVFARVS
                     PEHKLRLVSALQKRGHVVAMTGDGVNDAPALRRADMGIAMGITGTEVTKEAATMVLAD
                     DNFATIIEAVRRGRTIYDNIVKFVRFQLSTTLGFATIFLAASIFGIASGQPFTAIAIL
                     WVNIIMDGPPAMALGLDPVDERVMGREPRPPGEPILTRPRWIAIGQSAVVMAAGTLAV
                     LALAPGPEPIAGEATVAGTMAFNTFVLYQFFNILNVRSDQQTVFRRATFRNSKLWFAL
                     AAVIALQIGVTHFGPMQELFDTTSITAAQWLVCVGVASTVMLTEELRKIWRRSRTGEP
                     AT"
     gene            3314..3946
                     /locus_tag="VLR27_15290"
     CDS             3314..3946
                     /locus_tag="VLR27_15290"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSP04873.1"
                     /translation="MEQRPVDLTVAALAAGALALGAWEARRGELRAAEARWFSGVNGI
                     SERWLAPAWVVMQAGSLGGALGIGGAVAASGHRDLGRRLALVGSLAWAGSKLVKPLAN
                     RGRPSSVVEVARILGRAQTGLGYPSGHAAVAVAMATAAGSQVPPAWKVPTWSAVAVVA
                     AARIYVGAHLPLDVAGGAALGLATERIVRVVRGPAGRRAPQRAPGAAPAG"
     gene            complement(3885..4421)
                     /locus_tag="VLR27_15295"
     CDS             complement(3885..4421)
                     /locus_tag="VLR27_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04874.1"
                     /translation="MTARRTTAGLVTATLLLGGCAVGTGGAAPRCEPGMRLATLAQSV
                     PEAAYVPCVEELPAGWTFEGLHVDDDGATMTLESDRADRDVRVELAESCDIAEATPIT
                     PSDEGVRTYHRVDSIDPRYAGQLLDVFPGGCVTVDYDFERGRHVALVTELQAVVGLFT
                     RLELRQVLAAELDVRLDP"
     gene            complement(4418..6079)
                     /locus_tag="VLR27_15300"
     CDS             complement(4418..6079)
                     /locus_tag="VLR27_15300"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HSP04875.1"
                     /translation="METPPTLPLRSPTPGATAARRRPSGEAPPLPRPVSTATRASGAV
                     LVLVALVSLALGTDTGLRAITRADLAVLRALERLRWDPLVDAAEIVVAVGSTTAFRVL
                     AWVVLLVLLAVRRYQHLFATLALLLVVPLVAAVIRESVGRMRPAGVEILAGWDGYAHP
                     SRPVGQLALVLTIATVVLAPRGAWRARAVVATAAVLVLVAAARLYTAVDHPSDVAASV
                     ALGVAAPVLALHLLTPEEAFPVTYRRGVRAHLDVGGRRGEAIRRAFASQLDVDVVDVA
                     PFGQRGSAGSTPLRVATPSGALFAKLYAAVHLRSDRWYKLGRTVRYGRLEDERPFNSV
                     RRLVQYEDHMLRLMRDAGVPTAEPHGIVEITPEREYVLVTELIEGATHLTDGDVTDDV
                     VDQALAMVRTMWDAGLAHRDIKPSNLLLADGRVRLIDVAFAEVRPSPWRQAVDLANMM
                     LTLSLCVPPARVYERATRLFTPDEIAEAFAATRAVTMPAQLRAMLRERDDDVVEQLRR
                     LAPPRQPVAIQRWTLRRVGLTLAVLLGGVVALALVLANLDLVGLL"
     gene            6130..7005
                     /locus_tag="VLR27_15305"
     CDS             6130..7005
                     /locus_tag="VLR27_15305"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSP04876.1"
                     /translation="MVLLVLSACAGDGGRDADARAGLVVASFSFSESELVAEIYAQAL
                     EASGVTVQRQLDLGPRELVLPALRGGLVDVVPEYAGSALDAVVPASDGERGDVDAVIT
                     ALAEQVDPWGLSVLDPAAASNENVIAVRSDLAASLDLRSVSDLAGGASRFVLGGPPEC
                     PRRPRCLPGLEARYGLDFAGFEPFADAVLVRSALVDGVIDVGVLFSTDAAVTGDEVTV
                     LVDDRRLQPPDNVVPLVRTEALADRRVQATLDAVSAELTTDGLRLLNWRLANAGTDPA
                     SEARGWLIRHGLVDR"
     gene            complement(7019..7711)
                     /locus_tag="VLR27_15310"
     CDS             complement(7019..7711)
                     /locus_tag="VLR27_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04877.1"
                     /translation="MSVRQRSSEEREELAERIADRLDLPITAAGVVFLLLVLAETISE
                     PEGGVGTVFAIVSWGLWALFVGEFLLRLIVAPSTTTFLKRNWWQVFFLAVPALRFVRA
                     LRVLRAARVGRVLSSAVRSSRTAGRKLSSRLAALGVVTIVVILASSQLLFELGDYDSY
                     GDALDTAALATIAGEPFFPEHTALRVVQIIIITYSLVVFAALAGTLGAFLLERKADDD
                     RRLQAEAVPPPS"
     gene            complement(7737..9047)
                     /locus_tag="VLR27_15315"
     CDS             complement(7737..9047)
                     /locus_tag="VLR27_15315"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSP04878.1"
                     /translation="MVRSGRPALAGVLLLLAPLGGVAAACGGDRPEAAEELRGTRIEI
                     LAVWEDAEADAFRLVLDRFEEETGARVTFSSTAGTDMGTALDDRLASGDPPDVAVLPQ
                     PALLERYARTGEIVPVGDVVGAAVRAGWAPVWEQLATVDGVLYGVWFKAANKSLVWYS
                     LAAFEDAGLVPPADLDGLADAARDLAARGMPAFSLPGLPADAWVLTDWFENLYLRLAG
                     PDRYDALAAHRIPWTHPTVAETLQTMAALLDDELVTRVDDATFPDSVGDVFSPRPRAA
                     MVVEGDFVPGVVDDGGAAVVGIDVDVFAFPGQEPTDRAVVVGGDAAVLVQDTPGGRAL
                     LQFLASTRAAEVWAGLGGFISPNQGVDLGTYPDAPSRRIARALLEAGDDLRFDLSDLQ
                     PVPFGATTGAGLWAELSAFVADPTDIPGTTARIEAAADASWAGR"
     gene            9170..9904
                     /locus_tag="VLR27_15320"
     CDS             9170..9904
                     /locus_tag="VLR27_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP04879.1"
                     /translation="MRDVTLAAATVHASKIYGKDATEVRALDDVTVGFEAGRFTAIMG
                     PSGSGKSTLLHCIAGLDTLTSGSAYIGDTDLSTLDDNHLTVLRRDQVGFVFQAFNLVP
                     TLTADENVTLPLALAGRKGDRDWIDEVVRTVGLQDRLDHRPSELSGGQQQRVAVARAL
                     ASKPRIVFADEPTGNLDSRSGSDVLQFLRAAVDDFGQTIVMVTHDPVASSYADRIVFL
                     ADGRVVDEMLEPTAERVLDRMKQFGD"
     gene            9906..12455
                     /locus_tag="VLR27_15325"
     CDS             9906..12455
                     /locus_tag="VLR27_15325"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSP04880.1"
                     /translation="MLRTTLRNLAANKLRLLTTGFAVLLGVAFMSGTMILSATMDKTF
                     DDMFVNAYEGTDGMVRSSSVLETPEGLEIRPRIDESMVEVIEAVDGVAVAQADVWGTA
                     QVIDRDGDPVGSPEMGPPTLGATWPVDELNTWTLVDGRGPEADDEVAMDKKTADDAGY
                     RAGDQVRILVNGAPLEVTLTGVVRMADTDSPGGAQFSIFTVEGAQKHFGEPGKVDSIS
                     IAAEDGVSQTELVQRVSGVLGDDMEAITGDALVRENQDAMAEATAFFDQFMMIFAVVA
                     LLVGGFTIFNTFFITVAQRTKQHALMRAIGASRRQVLWSILLEAVVIGIIASVIGLGA
                     GVLIAAGLKAMLTAFGVALPAGGMVFGLGTAVVAFTAGLLVTVLSAVSPARKAGKVPP
                     IAAMRDIEVSSSGYGSKERIMVGLAVLALGVAGLLYGLFGSPSNGLAIVGGSAIVVFF
                     AVSVLGRTVSLPMSRVIGWPLAKGRGIAGHLARQNAMRSPKRTAATAAALMIGVGLVA
                     FITIFAASTKASFNETLDRAFTGDIVVTSPQQFGGGGGMSPELTERIRDLPEVETAAG
                     LRGGALEIGGATRFVLAADTAGFDLFDVEPVAGSPEDLDATSVAVHEDVAEDLALSVG
                     DMVPARFAATGAQELEIAMIYGENQPAGNWVLGIEAYEANFVDQLDAQIFIRSAGDAS
                     PEEALAAVEREASAFPGAKVLDQNEYKQEQLAFVDQMLGLVYAMLALAIIIALLGIGN
                     TLALSIFERTRELGVLRAVGMTRRQLRSTIRWESVIIAVQGAMLGLLIGVFFGWALVA
                     ALAEEGLTTFAIPVATLAWVVVLAALAGVVAAILPARRASRLDVLRAVVSE"
     gene            complement(12468..13613)
                     /locus_tag="VLR27_15330"
     CDS             complement(12468..13613)
                     /locus_tag="VLR27_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HSP04881.1"
                     /translation="MPHYRSVGTVPAKRHTKAFDEHGNRLFEELVGTEGFSRESALLY
                     HRCSPSAILGVEAVDPPETHPGGGIGPRHFRTGDLSTGQDLVTGRRRLLANADVTIAF
                     ARTSAPSPLYRSVVGDELVYLHEGTARLESVFGSMDVRSGDYVVIPAGTTHRWVPSSD
                     LVAALVVESSGHVRIPDKYLSPHGQLLEGAPFSERDLRGPDRPMRVDEADVPVLVRTR
                     TGWMRYRHATHPFDVVGWDGCVYPHALSIHDFEPIVGRIHQPPPVHQTWALPGAVVCS
                     FVPRPYDFDPDAVRVPYHHHNVDSDEVLFYAEGDFMSRSGSGIGRGSISLHPAGFVHG
                     PQPGSSDKADAADGTGEIAVMVDTFRPLEVLADAAGAEEPGYSSSWT"
     gene            complement(13613..14728)
                     /locus_tag="VLR27_15335"
     CDS             complement(13613..14728)
                     /locus_tag="VLR27_15335"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSP04882.1"
                     /translation="MRHAYGIVTGPDGGAHLAARDGDVAVDLRGVPGVPADVAEADDL
                     HPLLALGRTGWEDVHGAVHEHLASGPPTVPVADLQVRLPVVPGDYVDFYASEHHAANL
                     GRILRPSEPGLPDAWHHLPIGYHGRAGSIVVSGTPVRRPVGLVGPGDRRPSGRLDLEL
                     ELGFLVGTGVPMGEVVAPDDAAEHVFGVALVNDWSARDIQAFEYRPLGPFAGKSFATS
                     MAGWITPLDAIPRVSSPVQDPAPESGLSASRDWGLDLDVAWSLNGTEVGRTNASHLWW
                     TFAQQLAHLTANGAGLRAGDLFASGTISGPGRGEEGSLIEAARNGASPLTLDDGSTRC
                     WLEDGDTVTLSGSWCTAAGIVRLPEVTATIAPAQEVR"
     gene            complement(14737..15873)
                     /gene="hppD"
                     /locus_tag="VLR27_15340"
     CDS             complement(14737..15873)
                     /gene="hppD"
                     /locus_tag="VLR27_15340"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HSP04883.1"
                     /translation="MTTVERTAGTEAPATHLQGWDCIEWWVGNARSTAGFLMSAFGFE
                     CTGYAGPESGVPDRVSYVLEQGDIRFVVTGALQADSPIADHVRHHGDGVHDLAWIVDD
                     VDATFGAAVERGARPVREPWEETDELGTLRLAQIATYGDTVHTFVDRQRYAGEQAHRL
                     EPGFHDERLPPVPVGPDVGLQGLDHVVGNVEQGSLDQWVDFYARVLGFEQLVHFDDDQ
                     IVTEYSALMSTVVWDGSKIVMPINEPADGLKKSQIQEYLDCYDGPGVQHIAMRTDDIV
                     ATVDALRQRGVRFMEVPDTYYLEAKERLADFDLPWAELQRLSILADRDHDGYLLQIFT
                     ETVTDRPTVFFEIIERHGAKGFGEGNFKALFEAIERDQARRGNL"
     gene            complement(15870..16703)
                     /locus_tag="VLR27_15345"
     CDS             complement(15870..16703)
                     /locus_tag="VLR27_15345"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:NF012571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HSP04884.1"
                     /translation="MAELTIELPADHPGVSDEDYLARRARIAEVGRRYRPGEPIPEVH
                     YSPEEDQVWATVSAELAQLHQHFACAEVRDGSERLALPKDRVPQLHEVDARTRQLSDF
                     RIRPVPGLVPTREFYGSLADRTFLSTQYIRHHSVPFYTPEPDIVHEIIGHATMLASPR
                     LADLYERAGRASLRATSDEALDFFSQVFWFTLEFGVLHEDGEVRAYGAGLLSSYGEIQ
                     VFRDAEIRPWDIQAMGTTDYDITQYQPVLFAAESMDAVLADLGAFFDSYDDDTYRRNH
                     A"
     gene            16882..17079
                     /locus_tag="VLR27_15350"
     CDS             16882..17079
                     /locus_tag="VLR27_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04885.1"
                     /translation="MTDADVDPERLAVCAYWRCGVLFERRSINQRFCRKACRSRQGKW
                     QRAQERRAAREEAKQRRRRPS"
     gene            17076..17450
                     /locus_tag="VLR27_15355"
     CDS             17076..17450
                     /locus_tag="VLR27_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04886.1"
                     /translation="MTLSYRGAEQIAMGRDLMERNHLRPFERTVLRLTDSGMSTAEIA
                     WRFRRSPGHIERVIDLSRLPRSENEARTSDELRPVERCVLRARDEGVGTAEIAARLRR
                     TPGHVARVERYASFKLARAATS"
     gene            17447..17680
                     /locus_tag="VLR27_15360"
     CDS             17447..17680
                     /locus_tag="VLR27_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HSP04887.1"
                     /translation="MKVWIDQDLCTGDGLCEEIAPTVFAMLDDGLAYVKDGDKILSDP
                     GGAEGVAPIPDDQVEATIEAAEECPGECIFIEV"
     gene            complement(17667..18788)
                     /locus_tag="VLR27_15365"
     CDS             complement(17667..18788)
                     /locus_tag="VLR27_15365"
                     /inference="COORDINATES: protein motif:HMM:NF044385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSP04888.1"
                     /translation="MKILVTSTPGLGHILPMVPLVRELRDRGHELRWVGGHAGLDEIE
                     ALGIPVRRVGMPEADRQRTFFARHPEVSSLPPMDRQRFAFPRVFGEIAAAEMTEPVRA
                     AAEEWGPDVILHDAAELAAPLVAATLDIPSVSHGFGQIVPEPAVRTAGEVLAPRWWAA
                     GLEPDAHAGSYRGLYVDIYPPSLRSADMDHVPRVQRCRPADGAPSSGGAVYVTFGTLF
                     NQAEDALRTAVLAAADVADEVVVTLGGGRDPAVLGAVPDHVRVETFIPQADVLPRCAA
                     VVCHGGSGTVLAALAHAIPVVCIPQGADQFANAAEVERVGAGRSVAAGVTRAILRDAI
                     EHVLRAPEPRRSAAELAAEIAVMPDRTAVARAIEAVRPR"
     gene            complement(18870..20288)
                     /locus_tag="VLR27_15370"
     CDS             complement(18870..20288)
                     /locus_tag="VLR27_15370"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HSP04889.1"
                     /translation="MTDAGFRGTTVEQVVAQVTSREIGAREQVQRALDAIEALDPTVH
                     AFVAVDAERALVEAEEIDRRIAAGEEVGPLAGVPLAVKDVEDVAGYTTTNGSALHADD
                     PPAEQDSAHVARLRAAGCIVVGKTNSPEFGWQADTSNQVFPSTVNPWNPERSAGGSSG
                     GSGAAIAAGMVPLATGSDGGGSIRIPSAVHGLTGLKTSLGRVPGGGPTPARWMHLSCK
                     GPMARSARDVALALDAVVGFDPTDLHTLPPHHGPSWRSQVDEVLPPRRVVWSPTLGYG
                     RVDAEVAAACRAAVDRLADEGTEVIEVETVFDEDPAFVWAAVAYSAILRTVADRRGTP
                     EWETITPGLRAMFDLVAGQSGVDVLQAMDAGHRLNHRLVEVLHRGAVLLCPTVAGQTG
                     KVGEQGTIDGEPSMGWVSYTYPFNLTGSPAGTVCVGHTDDGMPIGLQVVGPRFGDVVA
                     LRVLTHLEDLLEVDTVAPLDWA"
     gene            20360..20875
                     /locus_tag="VLR27_15375"
     CDS             20360..20875
                     /locus_tag="VLR27_15375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP04890.1"
                     /translation="MAEWRIRPRTPDDDDRVRAVNAANVPEVGPLDEDRLALFAEAAP
                     HFDVIEVDGVVVGLFVGLVEGMPYASPNYRWFVDRHERFAYVDRIALEPEARGVGIAD
                     ELYERFERWASETGRPVVCAEVNVEPPNPRSMRFHERRGYAAVGELAPYGTQERVAMV
                     EKRLRGVPSAT"
     gene            20913..22133
                     /locus_tag="VLR27_15380"
     CDS             20913..22133
                     /locus_tag="VLR27_15380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HSP04891.1"
                     /translation="MEHVDIAVIGGGPAGAATAIRAARAGAKVVVFEKGPHGRDKVCG
                     DGLTPRAVGALQELEIPYEDEAHRIDGLRMIAGRQRRQLPWPEHGRFPSHGAVWPRKR
                     LDAHLVDAAEKAGAELVYETEALPILDGHVVRGVTAGGRTVHADLTVVAAGAQGKAAR
                     MLGAERDPDEPFGLAIRAYVESPRHADRHLEACLTLRDRSGTAVPGYGWMFPCGDGTV
                     NIGVGALSTMKRFTDLNLNTLLDDYQALVRDEWGVGEYLARPRAWRLPMSSVKRHGPG
                     WVAIGDAAGLVNPMNGEGIDYGLESGMLAADLFLESPSTAPARYDIEIGERFDAFLRT
                     GRRFSFLIGHPWILRNGLRVAVGTQKIADITLSVMGNLVDNETPGAAGRVMRIADRTL
                     AAADPLLRKTRAAS"
     gene            22267..25557
                     /locus_tag="VLR27_15385"
     CDS             22267..25557
                     /locus_tag="VLR27_15385"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HSP04892.1"
                     /translation="MPSIRRLLIANRGEIAVRIARTARAEGIQTVIAHAEDEADAPFV
                     RAGDAAQALAGTGPAAHLSVEGVVAAAVAAGCDAIHPGYGFLSENPELARACREAGIT
                     FVGPGVSQLETFGDKLRGRAVAEAAGVPVLAATAGATDLAAAESFFDEHGPLMVKAVA
                     GGGGRGMRVVTERAQLAEAMAACAREAAAAFGDDAVYVEELLTGARHVEVQIVGDGQR
                     VEHLYERDCTLQRRRQKVVETAPAWDLAPSIRVQLQDAAVRLGEHVDYDSLGTVEFLV
                     AGERIAFIECNARLQVEHTVTEAITGLDLVQLQLALAGGSSLSSLGMFATPPASGVAL
                     QARVLMESVGADGQAVPAGGTITGFHAPDGVRVDTFAEDPSNLAGYTTSPRYDSLLAK
                     VVVAGDEPDALLDAAASALDDLAIEGVETNAAFLAAVLRDPAVRDGDIDTTWLEANAA
                     RLGGGGATANRTAGAVVDRTDPLAVLDHGRPRDGATPALPEREDSTIAVRAPMQGTVG
                     SIAVSVGDEVAAGATVAVMEAMKMEHVITTSRSGIVRRIGVEVGDAVFAGHDLVVLEP
                     AEVAATAGLAEEDVDLDAIRPDLAEVIERHAHGLDENRPDAVEKRRKLGKRTARENVA
                     DLIDDGSLIEYGPLVVAAQKKRRSIEDLREKTPGDGLVGGVATVDGHPVAVASYDYTV
                     LAGTQGLNNHRKKDRLFEVAEHNRLPVVFFTEGGGGRPGDTDGTGIAGLDCLAFHYFG
                     KLSGLVPLVGVASGRCFAGNAAILGCCDVVIATRDSNIGMGGPAMIEGGGLGVYRPEE
                     VGPIEDQIASGVVDVAVEDEVEAIAVAKQYLSYFTEPDLGVWDCADQRWLRRAIPENR
                     LRVYEVRGVIETLFDADSVLELRRGFGHGMVTALARIEGRAVGVIANDPAHLAGAIDS
                     DAADKAARFMQLCDAHDLPIVFLCDTPGIMVGPDAERTGLVRHASRLFVTAGGLTVPY
                     MTVVLRKGYGLGAQAMAGGSFHAPLFVVGWPTSEFGGMGLEGAVKLGYRNELAAIEDP
                     EERRRTFDEMVERMYEVGKGTSMAAAFEIDDVIDPVDTRRWITTGLAAAPAPAPRTGK
                     KRPHIDTW"
     gene            25571..27283
                     /locus_tag="VLR27_15390"
     CDS             25571..27283
                     /locus_tag="VLR27_15390"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HSP04893.1"
                     /translation="MTTLDIPFDRYPRFDELSEHLQRLAAEYPDLLELDVLGQSHEGR
                     ELWIATVTNTATGPHHEKPAVWLDGNIHASEVTASVALLHLLTHLCTGHGTDQRVTRA
                     LDTRTFYVVPRVNPDGAELALSEVPFVVRSTTRAWPRPEQREGLVSGDVDHDGRSLQM
                     RIEDPNGTWKAHPSIPKLLVAREPDEDGPGPYYRLLREGHVEGYDGVNIPTAPPLAGI
                     DSNRNFPYQWRRFPEGEGFGWGHGDFPTSEPEVRAVVQGVVDRPNIGIYFAHHTYSGV
                     ILRPYSDRPDDAFPTVDKWTYDELGRRAEEITGYPYLGVYDGFRYDPKDVITGVGDDW
                     AYDHRGLFAWTTEFWNPLTAAGIEDPHPIEWYRDHTLDDELKLLAWVEENVPDGYVDW
                     YPFEHPQLGSVELGGWHAAAAFRNPPPHLLEAEVEPHSELAVFQALCLPELRVRDTSV
                     ERLGDDTWQVRVVVENAGWLPTNVTKRAEEAKVTTPIEARLVLPEGASLVHGTAQAEL
                     GQLSGRALRRSAVGMFGPPNDGTGDRASATWTVRAPAGTSLEVVVRSDRAGVVRATVE
                     LAID"
     gene            complement(27448..28056)
                     /locus_tag="VLR27_15395"
     CDS             complement(27448..28056)
                     /locus_tag="VLR27_15395"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HSP04894.1"
                     /translation="MTLRSIALNCTLKPGDAESSTELMLTQVLDELRSLDVETAPIVR
                     VADLDIKPGVTSDEGDGDEWPALRRQILDADILVVGTPIWLGQPSSVVKRVLERMDAF
                     LGEEDDRGRMISYDKVAIAAVVGNEDGAHHVSAELFQALNDVGYTIPAGGVTYWVGEA
                     MHGIDYKDKEPTPDVTAATNKTAAANAAHLARLLQRDGYPAT"
     gene            complement(28102..29103)
                     /locus_tag="VLR27_15400"
     CDS             complement(28102..29103)
                     /locus_tag="VLR27_15400"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HSP04895.1"
                     /translation="MRRRRLLGLAAATSLVLAACGSDASDVERSSDDTTEVTEPAPTT
                     TEAPTTTTTTVATTTTTAPPAPDTAPAPAASPAELTERLVRAEDAVRDPATPAAELDR
                     AAFDQQLLYRQLARTPEWEPEVTASLSEPYSAAAAANVAARREFRSMHRTLSDALPAW
                     RIVEPPSADELLSYYQEAEASFGVPWEILAAVNLVETGMGRIRGVSVAGAQGPMQFMP
                     ATWAAFGDGGDVNDYRDAIMGAARYLAHNGGGRGEIDNALWNYNHSDRYVRGVKHYAS
                     VMEQDPNTYRGYHQWEIVYLSTMGDVWLPVGYESEARVPVADHLGRNPGHHLGTATH"
     gene            complement(29154..30707)
                     /locus_tag="VLR27_15405"
     CDS             complement(29154..30707)
                     /locus_tag="VLR27_15405"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HSP04896.1"
                     /translation="MPSGLPRIGRATLVVVVIALAITVALTAAAQRAHDTNEDRLLAQ
                     QAEEATAVLQASVPRILSNVAQVARVVDLLDGDTEAMEAELAGDINEESFTTLTVLRR
                     TGAEPVVELGAPTVLDDDDLVATLQEVRPDPDLVHVSAFLDDDDDRRLAYGTDTGASD
                     LVVIAEVRLDGPRISDDRDGEAFDGIDLAIYLGEGERDEDLLTSTTPDLPIDGRRTSR
                     EVPFGDTQLLFVVTPAGTLGGGVLAVLPWLAALAGLVSGGIAAVLVESLHRRRLDAET
                     FTEELHELYQREHAIAHTLQHSLLPTHLDRLQGIEVAARYFPGAEGTDIGGDWYDVIN
                     DEDGTFTVVVGDVVGRGVKAAAVMAAMRYATHAVAGQSTEPGEILRAVNQLEHIRGDF
                     VTMLCGNVDPVARTICFATAGHPAPLLISPEETRFLRVSPGPPIGFLDDPTFPVKRTT
                     LPHGSVVVLFTDGLYERRGESIEVGLERLREAATRLDGTVDDILDGLAAAMLGDGVSD
                     DTAMLAFRV"
     gene            complement(30744..31178)
                     /locus_tag="VLR27_15410"
     CDS             complement(30744..31178)
                     /locus_tag="VLR27_15410"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP04897.1"
                     /translation="MHGNSATGRTQGEFAWRLGSGSGSIISAREAFDSWLRQTTTDAE
                     LLEDMSVVVSELVSNALDGAPPDEPDAEVRAEIDDHMLHLVVSNRLPDGVADIRHWDL
                     DDPLRGGGRGLMIVRAYTDSLAVDSTGGAVQVRCSRRLDPAS"
BASE COUNT         4387 a        10951 c        11562 g         4339 t
ORIGIN      
        1 aggcctggga cgcgctcgcc cgagtggacg cctaccgccg gtggtggccg tggttgcgcc
       61 ggttcgacgc cgaacggctg gcgtcgggcg agcggtggga ggcccgtatc cgcatcccga
      121 tgccgtggtc gctgcgcttc accctcgacc tccgcgacgt cgaggcgccg gtccgggtga
      181 tcgccgacgt caccggcgac atcgagggca cggcgacggt cacgatcgag cggggcggtg
      241 acggcgccga gatccggctc cgctccgacc tggcaccccg gcaccggctg ctgcggggcg
      301 tcaaccgcat cctccccggg gtgtcccggc gcttgcacga ccgcgtcgtg gaccgggcgt
      361 tccagcagtt cgccgaccga gaccgatccg gcgcggtgga gcggtaggct ccttcgtcga
      421 aggggagtat ccccccggtg cggtgcacca tcgctgtctc gtcactacgg ctccggcccg
      481 ggacatcggc ccacgacgtg ggtgggagag accttcggag cacggcccgt cgtcgaattc
      541 cgaggtgcaa ccccgttgtc cagcacgcct gcaaccgccc cgacccgcga ggtcgccgcg
      601 acgttccacg atcgcaccgt cgacgaggtg ctcgagaccc tctcggtgga tccgggcgcc
      661 ggtctgacca ccgacgaggt cgtccgccgg cgtgaggagc acgggtccaa cgagctcgac
      721 gaggcgcccc gcgacccatc gtggaggcgc ttcgctcgcc agttcaaaga cctgctgatc
      781 ctgatcctcc tggcggctgc gggcatcagc ttcgtcgtgt cgggagagct gaagacgccg
      841 atcgtggtgc tcgtcgtcgt cctgttgaac gcggtcatcg ggttcgtcca ggagaaccgg
      901 gcggaggcgt cgctggatgc gctgcgccgc atgctcgtgc tcgaggtgcg cgtccgccgc
      961 gacggcacca tgcgaacggt cccggcggcc gagctggtgc cgggtgacgt ggtggctgtg
     1021 gaagccggcg atcgcatccc ggccgacggc cgggtgatcg aggccgtgca gctggaggtg
     1081 gaggaagccg ccctcaccgg cgagagcctc ccggtcgcga aggtgcccga tcccgtgccg
     1141 ggcgaggacg tgccggtcgc cgatcgcacg tccatggccc acatgcagac caccgtcacc
     1201 cgtggccggg gcgagctcgt catcaccacg accgggatgg ggaccgagat cggccgcatc
     1261 gccggcctcc tccgcgaggc ggctccggag aagaccccgc tgcagcgcca gctcgatcac
     1321 ctcgcgcaca gcctggcgaa gctggcgggc gtcatcgtcg ggctggtctt cctcatcggg
     1381 ctgatccgcg gcgaggacat ctccgacctg ctcctcacgg ccgtcgccct ggcggtcgca
     1441 tcgatccccg agggcctgcc ggcggtcacc gccgtgaccc tggccatcgg tgtgtcgaag
     1501 atggctcggg agaacgccat cgtgaagcgc ctggccagcg tcgagaccct cggctccacc
     1561 accgtcatct gcagcgacaa gaccgggacg ctcacgctga accagatgac ggctgaggag
     1621 atcgtcgtga ccggacgccg cctggcgatc agcggcaacg gctacgcccc cgagggcgag
     1681 atccagggcc tcgacgacgt gccgatcgac ctcgacgagg tgctcgagcc catggcgctg
     1741 tgcagcgacg ccacggtgcg cttcgacgag gaggagggcg tgtggcgcct ggtgggcgac
     1801 cccacggaag gggcactggt cgtgctggcg ggcaaggggg gcgtcgatcc cgcgacccgg
     1861 cgggccgagc gcccacgggt gggcgaggtt ccgttcgact cggcccacaa gctcatggcc
     1921 acgctgcacg acagtgaatc cgatctgcgc atgtacgcca agggtgcacc cgatgccatc
     1981 ctcgaccggt ccaccaccgt gctgggctac gacggcacca tcgtccccat cgaggaggcc
     2041 cgcgaggagc tcgaggagca caacgctcgc ctggccagct ccggaatgcg ggtgctcgcg
     2101 gtcgctcggc gcgtcatctc ccgggatgag tggaagggag acggtgacct cgatgcggtc
     2161 accgatctga cgttcctcgc cctgatcggc atcgtcgacc cgccccggga ggaggcgaga
     2221 ttggcgatcg aggaggccgg tggcgcaggc atcagcgtga agatgatcac cggcgaccac
     2281 gccagcacgg cgaaggcgat cggcgagcag ctgagcctcg gtggcggaga ccaggtcgtg
     2341 gccgtgaccg gacgggacct cgacgcgatg agcgacgacg agctcgccga gcgcatcgag
     2401 gacatctccg tcttcgcccg ggtctcgccg gagcacaagc tccgtctcgt ctccgccctg
     2461 cagaagcggg gccacgtcgt ggccatgacc ggcgacggtg tgaacgatgc gccggccctg
     2521 cgccgggccg acatgggcat cgccatgggc atcaccggca cggaggtgac gaaggaggcg
     2581 gccacgatgg tcctcgccga cgacaacttc gccaccatca tcgaggccgt ccggcggggc
     2641 cgcacgatct acgacaacat cgtcaagttc gtgcggttcc agctgagcac cacgctcggc
     2701 ttcgcgacga tcttccttgc ggcatcgatc ttcggcatcg ccagcggtca gccgttcacc
     2761 gccatcgcca tcctctgggt gaacatcatc atggatggcc ctcctgcgat ggccctcggc
     2821 ctcgatccgg tcgacgagcg ggtgatgggc cgcgagcccc ggccaccagg cgagcccatc
     2881 ctcacccgcc cccggtggat cgcgatcggt cagtcggccg tggtcatggc ggcaggcacg
     2941 cttgccgtcc tcgcgctggc gcccggtccg gagccgatcg caggtgaggc cacggtggcc
     3001 gggaccatgg cgttcaacac cttcgtcctg taccagttct tcaacatctt gaacgtgcgc
     3061 agcgaccagc agacggtgtt ccgccgggcg acgttccgca acagcaagct ctggttcgcg
     3121 ctggccgctg tcatcgctct gcagatcggc gtgacccact tcggcccgat gcaggagctg
     3181 ttcgacacga cgtcgatcac ggccgcgcag tggctcgtgt gcgtcggcgt cgcatccacc
     3241 gtgatgctca ccgaggagct gcgcaagatc tggcgccgca gccgcacggg cgagcccgcc
     3301 acctgaggtc gggatggagc agcgtcccgt cgacctcacc gttgccgccc tcgccgccgg
     3361 cgcgctcgcc ctcggcgcgt gggaggcgag gcgaggggag ctgcgtgcgg ccgaggcccg
     3421 ttggttctcg ggcgtgaacg ggatcagcga gcgctggttg gctccggcgt gggtggtcat
     3481 gcaggccggc agcctgggtg gcgcgctcgg gatcggtggc gcggtcgccg cgtccggtca
     3541 ccgcgacctc gggcgtcgtc tggcgttggt cggctcgctg gcgtgggccg gctcgaagct
     3601 ggtgaagccc ctcgcgaacc gtggtcggcc gtcgtcggtg gtggaggtgg cccgcatcct
     3661 cggacgggcg cagaccggcc tggggtaccc gtccggtcac gccgcggtcg cggtggcgat
     3721 ggcgacggca gccggttccc aggtgccacc ggcctggaag gtgccgacgt ggtcggccgt
     3781 ggcggtggtg gccgcggccc gcatctacgt cggcgcccac ctgccgctcg acgtggccgg
     3841 aggtgccgcg ctcggcctcg cgaccgaacg gatcgtccgg gtggtcaggg gtccagccgg
     3901 acgtcgagct ccgcagcgag cacctggcgc agctccagcc gggtgaacag gccgacgacc
     3961 gcctggagct cggtgacgag ggcgacgtgg cgtccccgtt cgaagtcgta gtcgaccgtg
     4021 acgcagccgc ccgggaacac gtcgaggagc tggccggcgt agcgcgggtc gatcgagtcg
     4081 acccggtggt acgtgcgcac gccctcgtcg ctcggtgtga tcggggtggc ttcagcgatg
     4141 tcgcacgact cggcgagctc gacgcggacg tcgcggtcgg cgcgatcgga ctcgagcgtc
     4201 atggtggcgc cgtcatcgtc gacgtgcagg ccctcgaacg tccagcccgc gggcagctcc
     4261 tcgacgcagg gcacgtaggc ggcctcgggg acggactggg ccagggtggc gagtcgcatg
     4321 ccgggctcgc accgcggtgc ggcgcctccg gtgccgaccg cgcacccgcc gaggagcagc
     4381 gtggctgtga ccaggccggc ggtggtgcgg cgcgcggtca cagcagcccg accaggtcga
     4441 ggttggccag caccagagcg agcgccacca cgccgccgag cagcaccgcg agggtgaggc
     4501 cgacccggcg aagggtccag cgctggatgg ccaccggttg gcgtggcggc gcgagccgcc
     4561 gcagctgctc gaccacgtcg tcgtcccgct cccgcagcat cgcccggagc tgggccggca
     4621 tggtcaccgc tcgggtcgca gcgaacgcct cggcgatctc gtcgggggtg aacaggcggg
     4681 tcgctcgttc gtagacccgg gccgggggga cgcagagcga cagcgtgagc atcatgttgg
     4741 ccaggtccac ggcctgtcgc cacgggctcg gccgcacctc ggcgaaggcc acgtcgatga
     4801 gccgcacccg accgtcggcc aggagcaggt tggagggctt gatgtcccgg tgcgcgaggc
     4861 ccgcgtccca catggtccgc accatggcga gagcctggtc gacgacgtcg tcggtcacgt
     4921 cgccatcggt gaggtgggtg gcgccctcga tgagctcggt gaccaggacg tactcccgct
     4981 cgggggtgat ctccacgatg ccgtggggct cggcggtcgg cacgccggcg tcgcgcatga
     5041 gccggagcat gtggtcctcg tactgcacga ggcgacggac cgagttgaac gggcgctcgt
     5101 cctcgaggcg gccgtagcga acggtgcggc cgagcttgta ccagcgatcc gaccggaggt
     5161 ggacggccgc gtagagcttg gcgaacagcg cacccgacgg ggtggccacg cgcagcggcg
     5221 tggacccggc gctgccccgc tgcccgaacg gcgcaacgtc gaccacgtcg acgtcgagct
     5281 gggaggcgaa ggcccgccgg atcgcctcac cccgccgacc acccacgtcc aggtgggccc
     5341 gcacgccccg ccggtacgtg acggggaagg cctcctccgg cgtcagcagg tgcagggcca
     5401 ggaccggcgc ggccacgccg agggcgaccg atgctgcgac gtcggacggg tggtccacgg
     5461 cggtgtagag gcgtgcagcg gcgaccagca cgaggacggc tgccgtggcc accacggccc
     5521 gagcccgcca ggcgccccgc ggggccagga ccacggtggc gatggtgagc acgagcgcca
     5581 gctgccccac cggacgcgac gggtgggcgt agccgtccca gcccgccagg atctcgacac
     5641 cggcaggacg catgcggccc accgactcgc ggatcaccgc cgccacgagc ggcaccacca
     5701 ggagcagcgc caacgtggcg aacaggtgct ggtaccggcg caccgccagc aggacgagca
     5761 gcacgaccca cgccaggacc cggaacgcgg tggtcgagcc cacggcgacc acgatctcgg
     5821 cggcgtccac cagggggtcc catcgcagcc gctcgagggc gcgcagcacc gcaaggtcgg
     5881 cccgggtgat ggcgcgcagc ccggtgtcgg tgccgagcgc cagtgagacg agcgccacca
     5941 ggacgagcac cgcgccggag gcacgggtgg cggtgctgac gggacggggg agcggcggcg
     6001 cctctcccga cggtcgacgt cgcgcggcgg tcgcaccggg cgtcggggat cgcagcggga
     6061 gcgtgggtgg cgtttccatc ccgggtctgt ggcgatacta ggaccgtgcg atggcagggg
     6121 ttggccgcag tggtgctcct ggtgctgtcc gcctgcgccg gcgacggcgg ccgggacgcc
     6181 gacgcccggg ccggtctggt cgtcgcgtcg ttcagcttct ccgagagcga gctcgtcgcc
     6241 gagatctacg cccaggcgct cgaggcatcg ggggtcaccg tccagcggca gctcgatctc
     6301 ggcccgcgcg agctggtgct gcccgccctg cgcggtggcc tcgtggacgt cgtccccgag
     6361 tacgcaggct cggcgctcga tgcggtcgtg ccggcatccg acggcgagcg cggggacgtc
     6421 gacgccgtga tcacggcgct ggcggagcag gtcgacccgt ggggcctgtc ggtgctggac
     6481 cctgcagcgg cgtccaacga gaacgtgatc gccgtgcgct cggacctcgc ggcgtcgctc
     6541 gatctccgct cggtcagcga cctcgcaggg ggcgcgagcc ggttcgtcct cggcggtcca
     6601 ccggagtgcc cgaggcgccc acgctgcctc cccggattgg aggcccggta cggcctcgac
     6661 ttcgccggct tcgagccctt cgccgacgcc gtgctggtgc ggagcgccct ggtcgacggg
     6721 gtgatcgacg tcggggtctt gttcagcacg gacgccgcgg tcaccggcga cgaggtcacc
     6781 gtgctggtcg acgatcgccg gctccagccg cccgacaacg tggttcccct ggtccgcacg
     6841 gaggccctgg cggaccggcg ggtgcaggcg acgctggatg cggtgtcggc cgagctgacg
     6901 acggacggtc tccgcctcct caactggcgg ctcgccaacg cgggcaccga cccggcgtcg
     6961 gaggcccgcg gatggctcat ccgccacggg ctcgtcgacc gctgaggcct gacgtcgatc
     7021 agctcggcgg gggcaccgcc tcggcctgca gccggcggtc gtcgtcggcc ttgcgctcca
     7081 ggaggaacgc gcccagcgtc ccggccaggg cggcgaacac gacgagggag taggtgatga
     7141 tgatgatctg cacgacgcgc agcgcggtgt gctccgggaa gaacggctca ccggcgatgg
     7201 tcgcgagggc ggccgtgtcg agggcgtccc cgtaggagtc gtagtcgccg agctcgaaca
     7261 gcagctggct ggaggcgagg atgaccacga tcgtcacgac gcccagcgcc gcgaggcgac
     7321 tcgacagctt gcgtcctgcc gtccggctcg accgcaccgc cgagctcagc acccggccca
     7381 cccgagcggc gcgcaggacg cggagcgcac ggacgaagcg cagcgccggg acggcgagga
     7441 agaacacctg ccaccagttg cgcttcagga acgtcgtcgt cgagggggcg acgatcaggc
     7501 gcagcaggaa ctcgccgacg aagagcgccc acagccccca gctcacgatg gcgaagacgg
     7561 tgccgacgcc gccttccggc tccgagatcg tctcggcgag gacgagcagg aggaagacga
     7621 cgcctgcggc cgtgatgggc agatcgaggc gatcggcgat gcgctcggcc agctcctcgc
     7681 gctcctcgct gctgcgctgt cggacgctca cgctgcgagg atcgcacacg ccgccgtcaa
     7741 cggcccgccc atgacgcatc ggcggccgcc tcgatgcggg ccgtggtccc cgggatgtcc
     7801 gtcgggtcgg cgacgaaggc gctcagctcg gcccacagcc cggcgcccgt ggtcgcgccg
     7861 aagggaacgg gctggaggtc ggagaggtcg aatcgcagat cgtcgcccgc ctcgagcagc
     7921 gcgcgggcga tgcgtcggct gggagcgtcg gggtaggtgc cgaggtcgac cccctggttg
     7981 ggggagatga acccaccgag gccggcccac acctccgccg cccgggtcga ggcgaggaac
     8041 tgcagcagcg cccgtccgcc cggggtgtcc tgcacgagca ccgccgcgtc gccccccacc
     8101 accacggccc gatccgttgg ctcctgcccc gggaaggcga agacgtcgac gtcgatcccg
     8161 acgaccgcgg cgcctccgtc gtcgacgacg cccggcacga agtccccctc cacgaccatc
     8221 gcggcccgag gccggggcga gaagacgtcg ccgacggagt cggggaaggt ggcgtcgtcg
     8281 acccgggtga cgagctcgtc gtcgaggagg gcggccatcg tctgcagcgt ctcggcgacc
     8341 gtggggtggg tccacgggat ccggtgcgcg gccagggcgt cgtagcggtc gggtccggcg
     8401 aggcgcaggt acaggttctc gaaccagtcg gtgaggaccc aggcatcggc gggcaggccc
     8461 gggagcgaga acgcaggcat cccccgcgcc gcgaggtccc gggcggcgtc ggccaagccg
     8521 tcgaggtccg ccgggggcac caggcccgca tcctcgaagg cggcgagcga gtaccagacc
     8581 aggctcttgt tggctgcctt gaaccacacg ccgtagagca cgccatccac cgtggccagc
     8641 tgctcccaca cgggcgccca gccggcgcgc accgccgccc cgacgacgtc gccgacgggg
     8701 acgatctccc cggtccgggc gtagcgctcg agcagggcgg gctgggggag gacggcgacg
     8761 tcgggcgggt caccggatgc cagccggtcg tcgagggcgg tgcccatgtc ggtaccggcc
     8821 gtcgacgaga acgtgacgcg cgcgcccgtc tcctcctcga agcggtcgag gacgaggcgg
     8881 aaggcgtcgg cctctgcgtc ctcccacacg gccaggatct cgatcctggt gccccgcagc
     8941 tcctcggccg cttccggtcg atcgccacca catgcggcgg cgacgccgcc gagcggagcg
     9001 aggaggagca gcacgcccgc cagcgccgga cggcccgatc ggaccatgac cttggaccct
     9061 acgacgtgtt tgtgcgcagg agcacgatcg gggcacggct cgacagatga gcacgatcac
     9121 tacgggagca cccatgacga tcaccgaccg ttcgacatcg gatcgggggg tgcgagacgt
     9181 caccctcgcc gcagccaccg tgcacgcctc caagatctat ggcaaggacg ccaccgaggt
     9241 ccgtgccctc gacgacgtga ccgtcggctt cgaggccggc cggttcaccg ccatcatggg
     9301 accgtcgggc tcgggcaagt ccaccctcct gcactgcatc gccgggctgg acacgctcac
     9361 ctccgggtcc gcctacatcg gcgacaccga cttgagcacc ctggacgaca accacctgac
     9421 cgtgctgcgg cgcgatcagg tcggcttcgt gttccaggcg ttcaacctgg tgcccacgct
     9481 gaccgcggac gagaacgtca ccctccccct cgcgctggcc ggtcggaagg gcgaccggga
     9541 ctggatcgac gaggtcgtgc ggacggtggg cctccaggac cgcctcgacc accgcccgag
     9601 cgagctctcc ggtggccagc agcagcgggt ggccgtggcc agggccctcg ccagcaagcc
     9661 tcggatcgtc ttcgccgacg aaccgaccgg caacctcgac tcccgctcgg ggtcggacgt
     9721 cctgcagttc ctgcgtgccg cggtcgacga cttcggtcag accatcgtca tggtcaccca
     9781 cgacccggtg gcctcctcct acgccgatcg gatcgtgttc ctcgccgacg gcagggtcgt
     9841 cgacgagatg ctggaaccca cggccgagcg ggtgctcgac cgcatgaagc agttcgggga
     9901 ctgagatgct ccgcaccacg ctccgcaacc tggcggcgaa caagctccgg ttgctcacga
     9961 cgggcttcgc cgtgctcctc ggcgtcgcgt tcatgagcgg cacgatgatc ctcagcgcca
    10021 cgatggacaa gaccttcgac gacatgttcg tcaacgcgta cgagggcacc gacggcatgg
    10081 tccggtcgtc ctcggtgctc gagaccccgg agggcttgga gatccggccc cggatcgacg
    10141 agtcgatggt cgaggtcatc gaggcggtcg acggcgtggc ggtggcccag gcagacgtgt
    10201 ggggcaccgc ccaggtgatc gaccgcgacg gcgaccccgt cggcagcccc gagatgggcc
    10261 ctcccaccct cggcgcgacc tggcccgtgg acgagctgaa cacgtggacc ctggtggacg
    10321 gacgcggccc cgaggccgat gacgaggtcg ccatggacaa gaagacggcg gacgacgccg
    10381 ggtaccgggc cggtgaccag gtccggatcc tcgtcaacgg cgcgccgctc gaggtgacgc
    10441 tcacgggcgt cgtccgcatg gccgacacgg attcaccggg cggtgcgcag ttctcgatct
    10501 tcacggtgga gggcgcccag aagcacttcg gtgaacccgg caaggtcgac agcatctcca
    10561 tcgccgccga ggacggggtg tcccagaccg agctggtgca gcgggtctcc ggggtgctgg
    10621 gtgacgacat ggaggcgatc accggtgacg cgctcgtgcg ggagaaccag gacgccatgg
    10681 ccgaggcgac ggcgttcttc gaccagttca tgatgatctt cgccgtggtc gccctgctgg
    10741 tgggcggctt cacgatcttc aacaccttct tcatcaccgt ggcgcagcgc acgaagcagc
    10801 acgccctcat gcgggccatc ggtgcgagcc gccgccaggt gctgtggtcg atcctcctcg
    10861 aggccgtggt gatcgggatc atcgcctccg tcatcgggct cggcgcgggt gtgctcatcg
    10921 ctgcagggct caaggcgatg ctcaccgcct tcggggtggc gctcccggcc ggcggcatgg
    10981 tcttcgggct cggcaccgcg gtcgtcgcgt tcaccgccgg tctgctcgtg acggtgctgt
    11041 cggccgtctc cccggcccgc aaggccggca aggtcccgcc gatcgctgcg atgcgagaca
    11101 tcgaggtcag cagcagcggc tacgggtcca aggagcggat catggtcggc ctcgccgtcc
    11161 tcgccctcgg tgtggccggc ctgctctacg ggctgttcgg ctcgccctcg aacggcttgg
    11221 ccatcgtcgg tggctcggcc atcgtggtgt tcttcgcggt ctcggtgctg gggcggaccg
    11281 tgtccctgcc gatgagccgg gtgatcggct ggccgctggc caagggacgc ggcatcgccg
    11341 ggcacctcgc ccgccagaac gccatgcgca gcccgaagcg gaccgcggcc accgcggcag
    11401 ccctgatgat cggcgtcggg ctggtggcgt tcatcacgat cttcgcggcg tcgaccaagg
    11461 cctcgttcaa cgagaccctc gaccgggcgt tcaccggcga catcgtggtc acctcgcccc
    11521 agcagttcgg cggcggtggg ggcatgtcgc ccgagctgac cgagcggatc cgcgacctcc
    11581 ccgaggtcga gacggcagcc ggcctgcggg gcggcgcact cgagatcggc ggtgcgacga
    11641 ggttcgtgct cgccgccgac acggccggct tcgacctctt cgacgtggag cccgtcgccg
    11701 ggtctccgga ggacctcgac gccacgtcgg tcgccgtgca cgaggacgtg gccgaagacc
    11761 tggcgctgtc tgtcggcgac atggttccgg cccgcttcgc cgccaccggc gcgcaggagc
    11821 tggagatcgc aatgatctac ggcgagaacc agccggcggg gaactgggtc ctcggcatcg
    11881 aggcctacga ggccaacttc gtcgaccagc tggacgccca gatcttcatc cgcagcgccg
    11941 gcgatgcgtc tccggaggag gcccttgccg cggtggagcg ggaggcctcg gcgttcccgg
    12001 gagcgaaggt gctggaccag aacgagtaca agcaggagca gctcgccttc gtcgaccaga
    12061 tgctcggact ggtgtacgcc atgctcgccc tggccatcat catcgccctg ctcggcatcg
    12121 ggaacacgtt ggccctgtcc atcttcgagc ggacccggga gctgggcgtg ctccgagcgg
    12181 tcggcatgac ccggcgccag ctccgctcga ccatccgctg ggagtcggtg atcatcgccg
    12241 tgcagggcgc catgctcggc ctgctcatcg gcgtcttctt cgggtgggcg ctggtcgccg
    12301 ccctggccga ggaggggctc acgaccttcg ccatcccggt cgccacgctg gcctgggtcg
    12361 tcgtccttgc cgccctggcc ggcgtggtcg ccgcgatcct gccggcccgg cgggcgtcgc
    12421 ggctcgacgt cctgcgagcg gtggtcagcg agtaggcgag tcgtggctca ggtccaggac
    12481 gacgagtagc ccggctcctc ggcgccggcc gcgtcggcga gcacctcgag cgggcggaac
    12541 gtgtcgacca tgaccgcgat ctcgccggtg ccgtcggcgg cgtcggcctt gtcggacgag
    12601 cccggttggg gtccgtgcac gaacccggcc gggtgcaggg agatcgaccc ccggccgatg
    12661 ccggaaccgg agcggctcat gaagtcgccc tcggcgtaga agaggacctc gtcgctgtcg
    12721 acgttgtggt ggtggtaggg cacccgcacg gcgtcggggt cgaagtcgta gggccgcggc
    12781 acgaacgagc acacgaccgc accggggagc gcccaggtct ggtgcaccgg cggtggctgg
    12841 tggatgcgcc cgacgatggg ctcgaagtcg tggatgctga gcgcgtgggg gtacacgcat
    12901 ccgtcccacc cgacgacgtc gaaggggtgg gtggcgtggc ggtaccgcat ccaccctgtc
    12961 cgggtccgca cgagcacggg gacgtcggcc tcgtcgaccc gcatcggtcg gtcggggcct
    13021 cgcaggtctc gctcggagaa cggtgctccc tccagcagct ggccgtgggg tgacaggtac
    13081 ttgtcgggga tccggacgtg gccgctcgac tcgacgacca acgcagcgac gaggtcgctg
    13141 gacggcaccc agcgatgggt ggtgccggcg gggatcacca cgtagtcacc cgacctgacg
    13201 tccatcgatc cgaacaccga ctcgagccgg gccgtgccct cgtggaggta gaccagctcg
    13261 tcgcccacga ccgagcggta cagcggcgac ggcgccgagg tgcgggcgaa cgcgatggtg
    13321 acgtcggcgt tggcgaggag gcggcggcgg ccggtgacca ggtcctgtcc cgtcgacagg
    13381 tccccggtgc ggaagtggcg cgggccgatg ccgccgcccg ggtgggtctc tggcgggtcc
    13441 acggcctcca cgccgaggat cgccgacggt gagcaacggt ggtacagcag ggcggactcc
    13501 cgggagaacc cctcggtgcc cacgagctcc tcgaacaggc ggttgccgtg ctcgtcgaag
    13561 gccttggtgt ggcgcttggc gggcacggtg ccgacggatc ggtagtgggg catcagcgga
    13621 cctcctgggc gggggcgatg gtggcggtga cctcgggcag ccggacgatc cccgcggcgg
    13681 tgcaccagct gccgctgagt gtgaccgtgt cgccgtcttc gagccagcac cgcgtgctgc
    13741 cgtcgtcgag cgtgaggggc gacgcgccgt tgcgcgccgc ctcgatgagc gaaccctcct
    13801 cgccccgacc ggggccggag atcgtgccgg aggcgaacag gtcgccggcg cggaggccgg
    13861 cgccgttggc ggtgaggtgg gcgagctgct gggcgaaggt ccaccacagg tgggacgcgt
    13921 tcgtgcgtcc cacctccgtg ccgttcagcg accacgcaac gtcgaggtcg aggccccagt
    13981 cgcggctcgc tgagagaccg ctctcgggtg ccgggtcctg cacaggcgac gacacacggg
    14041 ggatcgcgtc gagcggcgtg atccagccgg ccatcgacgt ggcgaagctc ttgccggcga
    14101 aggggccgag cggccggtac tcgaaggcct ggatgtcgcg ggccgaccag tcgttcacca
    14161 gggcgacgcc gaagacgtgc tcagccgcgt cgtccggcgc gacgacctcg cccatcggca
    14221 cgcccgtgcc cacgaggaac ccgagctcga gctccaggtc gaggcggccg gaagggcgcc
    14281 ggtctcctgg gccgacgagg ccgacggggc ggcggacggg cgtcccggac accacgatcg
    14341 agccggcccg gccgtggtac ccgatgggca gatggtgcca ggcgtcgggg aggccgggct
    14401 cgctcgggcg caggatccgt cccaggttcg cggcgtggtg ctcggatgcg tagaagtcga
    14461 cgtagtcgcc cggcaccacc ggcagccgga cctggagatc ggcgacgggc acggtgggtg
    14521 ggccggaggc gaggtgctcg tggacggcgc cgtgcacgtc ctcccaaccc gtgcggccga
    14581 gggccagcag cgggtggagg tcgtcggcct cggccacgtc ggctggcacg ccggggacgc
    14641 cccgcaggtc gacggcgacg tctccgtcgc gggccgcgag gtgcgcgcct ccgtccggtc
    14701 cggtgacgat gccgtaggcg tggcgcacgg accgcctcag aggttgccgc gccgggcctg
    14761 gtcgcgctcg atggcctcga acagggcctt gaagttgccc tcgccgaagc ccttggcccc
    14821 gtgccgctcg atgatctcga agaagaccgt gggtcggtcg gtgaccgtct cggtgaagat
    14881 ctgcagcagg tagccgtcgt ggtcgcggtc ggcgaggatc gacaggcgct ggagctcggc
    14941 ccaggggagg tcgaagtcgg ccaggcgctc cttggcctcg aggtagtagg tgtcgggcac
    15001 ctccatgaac cgcacgcccc gctggcgcag cgcgtcgacc gtggcgacga tgtcgtcggt
    15061 gcgcatggcg atgtgctgca cgccgggacc gtcgtagcag tcgaggtact cctggatctg
    15121 gctcttcttc agcccgtcgg ccggctcgtt gatgggcatc acgatcttgg agccgtccca
    15181 caccaccgtg gacatgagcg cggagtactc ggtgacgatc tggtcgtcgt cgaagtggac
    15241 gagctgctcg aacccgagca cccgggcgta gaagtcgacc cactggtcga gactgccctg
    15301 ctcgacgttg cccacgacgt ggtcgaggcc ctggaggccg acatcggggc cgaccggcac
    15361 gggcgggagg cgctcgtcgt ggaagccggg ctcgaggcga tgggcctgct cgccggcgta
    15421 gcgctggcgg tcgacgaagg tgtgcacggt gtcgccgtag gtggcgatct gggcgaggcg
    15481 gagcgtgccg agctcgtcgg tctcctccca cggctcgcgc acgggacggg cgccgcgctc
    15541 gaccgcggcg ccgaaggtgg catcgacgtc gtcgacgatc caggccaggt cgtgcacgcc
    15601 gtcgccgtgg tggcggacgt ggtcggcgat gggggagtcg gcctgcaggg cgccggtgac
    15661 gacgaagcgg atgtcgccct gctcgaggac gtagctgacg cggtcgggca cgccggactc
    15721 cggcccggcg tagccggtgc actcgaagcc gaacgcggac atgaggaagc cagcggtgct
    15781 gcgggcgttg ccgacccacc actcgatgca gtcccaaccc tgcaggtggg tggccggggc
    15841 ctcggtgccc gcggtgcgct cgacggtggt catgcgtggt tcctccggta ggtgtcgtcg
    15901 tcgtaggagt cgaagaaggc gccgaggtcg gcgagcacgg catccatgct ctcggcggcg
    15961 aacagcaccg gctggtactg ggtgatgtcg tagtcggtgg tgcccatcgc ctggatgtcc
    16021 cacgggcgga tctcggcgtc gcgaaagacc tggatctcgc cgtaggacga cagcagtccg
    16081 gcgccgtagg cccggacctc accgtcctcg tggagcaccc cgaactcgag ggtgaaccag
    16141 aagacctggc tgaagaagtc gagcgcctcg tcggacgtgg cccgcagcga tgcccgcccg
    16201 gcgcgctcgt acaggtcggc gaggcggggg gaggcgagca tcgtggcgtg gccgatgatc
    16261 tcgtgcacga tgtcgggctc gggtgtgtag aagggcaccg agtggtggcg gatgtactgc
    16321 gtggacagga acgtccggtc ggccagcgac ccgtagaact cgcgggtcgg caccagcccg
    16381 gggacgggcc ggatgcggaa gtccgacagc tgccgcgtgc gggcgtcgac ctcgtgcagc
    16441 tggggcaccc ggtccttcgg gagggcgagg cgctcgctgc cgtcgcgcac ctcggcgcag
    16501 gcgaagtgct ggtggagctg ggccagctcg gcggacaccg tcgcccagac ctggtcctcc
    16561 tcgggggagt agtgcacctc ggggatcggc tcccccgggc ggtaccggcg cccgacctcg
    16621 gcgatgcggg cgcggcgagc caggtagtcc tcgtcgctga cgccggggtg gtccgccggc
    16681 agctcgatcg tgagctcggc catgccccca tggtgacgga gttcacaccg tttgccaacc
    16741 agttcgtcca atgatctcga tcagctctgc gcggagtgcg cagtcgactg cgtccagtgg
    16801 tcgcagatcg cccggtcagc gtgcgatctg cgctcgtgcc ggttgactgg tcccatcgca
    16861 gagcccaccg acgacgatga cgtgaccgat gccgacgtcg atcccgagcg gctggcggtg
    16921 tgcgcctact ggcgctgcgg ggtgctgttc gagcgacgct ccatcaacca gcggttctgc
    16981 cggaaggcgt gtcgcagccg gcagggcaag tggcaacggg cgcaggagcg ccgagccgcg
    17041 cgtgaggagg cgaagcagcg ccggaggcgg ccgtcgtgac actgtcgtac cgtggagccg
    17101 aacagatcgc gatggggaga gacctgatgg aacgcaacca cctgcgcccg ttcgaacgga
    17161 ccgtgctgcg cctgaccgac tccgggatgt cgacggccga gatcgcatgg cggttccgtc
    17221 gcagcccggg gcacatcgaa cgggtcatcg acctgagccg cctgccacgt tcggagaacg
    17281 aggcacggac gtccgacgag ctgcgcccgg tcgagcgatg cgtcctccgt gcccgcgacg
    17341 aaggtgtcgg caccgcggag atcgccgctc ggttgcggcg cacacctggc catgtcgccc
    17401 gggtcgaacg gtatgcgtcc ttcaagctgg cgagggcggc cacgtcatga aggtgtggat
    17461 cgatcaggac ctgtgcaccg gggacggact gtgcgaggag atcgcaccca ccgtgttcgc
    17521 catgctcgac gatggtctcg cctacgtgaa ggacggcgac aagatcctca gcgaccccgg
    17581 tggcgccgaa ggggtggccc ccatccccga cgaccaggtc gaggccacca tcgaagccgc
    17641 cgaggaatgc cccggcgagt gcatcttcat cgaggtctga ccgcttcgat cgctcgagcg
    17701 acggcagtgc ggtcgggcat gaccgcgatc tctgcggcca gctcggccgc tgaccgtcgt
    17761 ggttccgggg cacggaggac gtgctcgatc gcgtcgcgga ggatggctcg ggtgacgccg
    17821 gcggcgacgg accggccggc gccgacccgc tcgacctcgg cggcgttggc gaactgatcg
    17881 gcgccctggg ggatgcacac gaccgggatg gcgtgggcga gggcggcgag gacggtgccc
    17941 gaacctccgt ggcagacgac ggccgcgcag cggggtagca cgtcggcctg ggggatgaac
    18001 gtctccaccc ggacgtggtc gggtacggca ccgagcacgg cggggtcccg cccgccgccg
    18061 agcgtcacca cgacctcgtc ggcgacgtcg gccgcggcga gcacggcggt gcgcagggcg
    18121 tcctcggcct ggttgaacag cgtcccgaac gtcacgtaca ccgcaccccc gctcgacggc
    18181 gccccgtccg ccggccggca ccgctggacg cgtggcacgt ggtccatgtc cgcgctgcgc
    18241 agcgacggcg ggtagatgtc gacgtacagc ccccggtagc tgcccgcgtg ggcgtcgggt
    18301 tccaaccccg ccgcccacca ccggggcgcc agcacctcgc cggcggtccg caccgcaggt
    18361 tcgggcacga tctgcccgaa gccgtggctc acggacggga tgtcgagggt ggcggccacg
    18421 agcggggcgg ccagctcggc ggcgtcgtgg aggatgacgt cgggacccca ctcctcggcg
    18481 gcagcgcgga ccggctcggt catctccgca gccgcgatct caccgaagac gcgcgggaac
    18541 gcgaagcgct gccggtccat gggcggcagc gacgacacct ccgggtgacg ggcgaagaag
    18601 gttcgttggc ggtccgcctc cggcatgccg acgcgacgga cggggatgcc cagcgcctcg
    18661 atctcgtcca ggccggcatg gccgccgacc catcgcagct cgtggcctcg gtcgcgcagc
    18721 tcccgcacga gcggaaccat cggcaggatg tggccgaggc ccggcgtcga cgtcaccagg
    18781 atcttcatgg tcgtgcctca gcccgacgga cgctccgtca ccggtcgatc atccacccgg
    18841 accccggtcc tgaccagtcc gatcggcgct catgcccagt cgagcggggc cacggtgtcg
    18901 acctcgagca ggtcctcgag gtgggtgagc acccgcagcg cgacgacgtc gccgaagcgt
    18961 ggtccgacca cctgcaggcc gatcggcatc ccgtcgtccg tgtgcccgac gcagaccgtt
    19021 ccggccgggc tccccgtgag gttgaagggg tacgtgtacg acacccagcc catggatggc
    19081 tcgccgtcga tggtgccctg ctcaccgacc ttcccggtct ggcccgcgac cgtcgggcac
    19141 aggagcacgg ccccgcggtg cagcacctcg accagccggt ggttgagccg atggccggcg
    19201 tccatcgcct gcagcacgtc gacgccgctc tggccggcga cgaggtcgaa catggcgcgc
    19261 aggccgggtg tgatggtctc ccactccggc gtgccccgcc gatccgcgac cgtgcggagg
    19321 atggcggagt aggcgacggc ggcccacacg aaggcggggt cctcgtcgaa gacggtctcg
    19381 acctcgatga cctcggtgcc ctcgtcggcc aggcggtcga cggcggcgcg gcacgcggcg
    19441 gcgacctccg cgtcgacccg tccgtagccc agcgtcggcg accacacgac ccggcgcggt
    19501 ggcagcacct cgtcgacctg ggaccgccac gacggtccgt ggtgcggcgg cagcgtgtgc
    19561 aggtccgtgg ggtcgaagcc gacgacggcg tcgagggcga gggcgacgtc gcgggcgctg
    19621 cgggccatgg ggcccttgca ggacaggtgc atccaccggg ccggggtcgg cccgccacca
    19681 ggcacgcgcc cgagggaggt cttcagaccg gtgaggccgt gcaccgcgct ggggatgcgg
    19741 atcgagccgc cgccatcgga gccggtggcc agggggacca tgccggcggc gatcgcggcc
    19801 ccggatccgc cggaggaccc tcccgcgctg cgctccgggt tccacgggtt caccgtggac
    19861 gggaagacct ggttggacgt gtcggcctgc cacccgaact cgggcgagtt ggtcttgccg
    19921 acgacgatgc agccggcggc gcgcaggcga gccacgtgcg cagagtcctg ctcggccggc
    19981 gggtcgtcgg cgtggagcgc ggagccgttg gtggtggtgt acccggcgac gtcctcgacg
    20041 tccttcaccg ccaacggcac gccggcgagg ggaccaacct cctcgcccgc cgcgatgcgc
    20101 cggtcgatct cctccgcctc caccagcgcg cgctcggcgt cgacggccac gaaggcatgg
    20161 acggtcgggt cgagcgcctc gatggcgtcg agcgctcgct ggacctgttc gcgcgcgccg
    20221 atctcgcggc tggtgacctg ggccaccacc tgctcaacgg tcgtcccccg gaaccctgca
    20281 tcggtcatgg cgggacggta gtgccgaagc gccgggtgcg ccctcggccg ccgggcgtcg
    20341 ccggtggcag gctcctgcga tggccgagtg gcgcatccgt ccccgcaccc ccgacgacga
    20401 cgaccgggtc cgtgcggtca acgccgccaa cgtccccgag gtcggcccgc tcgacgagga
    20461 ccgcctcgct ctgttcgccg aggccgcccc gcacttcgac gtgatcgagg tcgacggcgt
    20521 ggtggtcggg ctcttcgtgg gactcgtcga ggggatgccg tacgccagcc cgaactaccg
    20581 ctggttcgtc gatcgccacg agcgcttcgc ctacgtcgac cgcatcgcgc tcgaaccgga
    20641 ggcgcgcggt gtcggcatcg cggacgagct ctacgagcgg ttcgagcgct gggcgagcga
    20701 gaccggtcga ccggtggtct gcgccgaggt caacgtcgag ccgcccaacc cgcggtcgat
    20761 gcggttccac gaacgtcgcg gctacgccgc cgtcggcgag ctcgccccct acggcaccca
    20821 ggagcgggtc gcgatggtgg agaagcgcct ccgcggggtg ccgtcggcca cctgactggg
    20881 ggaacgcccc ggcgacccgt gagaatggcc cgatggagca cgtcgacatc gccgtcatcg
    20941 gtggaggtcc cgccggcgcg gcgaccgcca tccgggccgc ccgggccggc gccaaggtcg
    21001 tcgtgttcga gaagggcccc cacggtcggg acaaggtctg cggcgacggg ctgaccccgc
    21061 gggcggtcgg tgcgctccag gagctggaga tcccctacga ggacgaggcg caccgcatcg
    21121 acgggctgcg catgatcgcc ggccggcagc gacgccagct gccctggccc gagcacggcc
    21181 gcttcccgtc ccacggcgcg gtgtggcccc gcaagcgcct cgacgcccac ctggtcgacg
    21241 ccgccgagaa ggccggcgcc gagctcgtct acgagaccga ggccctcccg atcctcgacg
    21301 gccatgtcgt gcgcggcgtc accgccggcg ggcgcaccgt gcacgccgac ctcaccgtcg
    21361 tcgccgccgg cgcccagggc aaggccgccc gcatgctcgg cgccgagcgc gaccccgacg
    21421 agcccttcgg cctggcgatc cgggcctacg tcgagtcccc ccgccacgcc gaccgccacc
    21481 tcgaggcctg cctcaccctg cgcgatcgca gcggtaccgc ggtccctggc tacggatgga
    21541 tgttcccctg cggggacggc acggtgaaca tcggcgtcgg cgcgctgagc acgatgaagc
    21601 ggttcaccga cctgaacctc aacacgctgc tggacgacta ccaggccctc gtgcgcgacg
    21661 agtggggtgt gggcgagtac ctcgcccggc cgcgggcgtg gcggctgccg atgtcctcgg
    21721 tgaagcggca cggacccggg tgggtggcga tcggcgacgc cgccggcctg gtgaacccga
    21781 tgaacggcga gggcatcgac tacggcctcg agtccgggat gctcgccgcc gacctgttcc
    21841 tcgagagccc ctccaccgca ccggcccggt acgacatcga gatcggcgag cgcttcgacg
    21901 ccttcctgcg caccggccgc cgcttctcgt tcctcatcgg ccatccgtgg atcctgcgca
    21961 acggcctgcg cgtcgcggtc gggacgcaga agatcgccga catcacgctg tcggtcatgg
    22021 gcaacctcgt cgacaacgag acgcctggcg ccgccggccg ggtgatgcgc atcgccgacc
    22081 gcaccctcgc cgccgccgac ccgctcctcc gcaagacccg cgccgccagc tgacgcttcc
    22141 tcacgcgcgg actgaccgcc gacggtcgat ctgcgcgcgg agaacgacga ccggcgggtt
    22201 ccatgcgcgc cgatcgactc gtggaggtcg gatggcgcgc gggaaacggt ggggcaggat
    22261 gggctcgtgc cgagcatccg acgcctgctg atcgccaacc gcggggagat cgccgtccgc
    22321 atcgcccgga ccgcccgggc cgaggggatc cagaccgtga tcgcccacgc cgaggacgag
    22381 gccgacgccc cgttcgtccg ggcgggtgac gccgcccagg cgctggccgg caccgggccg
    22441 gccgcccacc tgtcggtcga gggcgtggtc gctgcagcgg tggcggcggg ctgcgacgcg
    22501 atccaccccg gctacggctt cctgagcgag aaccccgagc tggcccgggc gtgccgcgag
    22561 gccggcatca ccttcgtggg tcccggcgtc agccagctcg agacgttcgg cgacaagctg
    22621 cggggacggg cggtcgccga ggccgccggc gtccccgtgc tcgccgccac cgccggggcg
    22681 accgatctcg ccgctgcgga gtcgttcttc gacgagcacg gccccctcat ggtgaaggcc
    22741 gtcgcagggg gcggcgggcg gggcatgcgc gtcgtgaccg agcgggccca gctcgccgag
    22801 gccatggcgg cgtgcgcccg agaggcggcg gccgcgttcg gcgacgacgc cgtctacgtc
    22861 gaggagctgc tcacgggtgc ccgccacgtc gaggtccaga tcgtcggcga cggccagcgg
    22921 gtcgagcacc tgtacgagcg ggactgcacg ctgcagcgcc ggcggcagaa ggtggtcgag
    22981 accgcaccgg cctgggacct ggcgccgtcg atccgggtgc agctgcagga cgccgccgtc
    23041 cgcctgggcg agcacgtcga ctacgactcg ctcggcaccg tggagttcct cgtggcgggg
    23101 gagcgcatcg cgttcatcga gtgcaacgcc cggctgcagg tcgagcacac cgtcaccgag
    23161 gcgatcaccg ggctcgatct cgtgcagctc cagctggcgc tggcgggcgg gtcgtcgctg
    23221 agctcgctcg gcatgttcgc gacgccgccg gcgtcggggg tcgcgctcca ggcgcgcgtc
    23281 ctgatggagt cggtcggcgc cgacggccag gcagtaccgg ccggcggcac catcaccggg
    23341 ttccacgcgc cggacggcgt gcgggtcgac accttcgccg aggacccgtc caacctcgcc
    23401 ggctacacga cgagcccgcg ctacgactcg ctgctggcca aggtcgtcgt cgccggcgac
    23461 gagccggacg ccctgctcga cgccgcggcg agtgccctgg acgacctcgc catcgagggc
    23521 gtggagacca acgccgcgtt cctcgccgcc gtgctccggg atcccgccgt gcgcgacggc
    23581 gacatcgaca ccacgtggtt ggaggccaac gccgcccggc tcgggggcgg tggtgccacg
    23641 gccaaccgca ctgccggcgc ggtggtcgat cgcaccgacc cgctcgccgt cctcgaccac
    23701 ggccggcccc gggacggcgc tacacccgca ctgccagaac gggaggactc cacgatcgcc
    23761 gtgcgtgcgc cgatgcaggg cacggtcggc tcgatcgcgg tgtcggtcgg ggacgaggtc
    23821 gccgccggcg ccaccgtcgc ggtgatggag gccatgaaga tggagcacgt catcaccacc
    23881 tcgcgcagcg gcatcgtgcg ccgcatcggg gtggaggtcg gcgacgcggt cttcgccggc
    23941 cacgacctgg tggtgctcga gccggccgag gtggcagcca ccgccggcct ggccgaggag
    24001 gacgtcgacc tcgacgcgat ccgccccgac ctggccgagg tcatcgaacg gcacgcccac
    24061 ggcctggacg agaaccggcc cgacgcggtc gagaagcgcc gcaagctcgg gaagcgcacg
    24121 gcgcgggaga acgtggccga cctgatcgac gacgggtcgc tcatcgagta cggcccgctc
    24181 gtcgtcgccg ctcagaagaa gcgacgctcc atcgaggacc tgcgggagaa gaccccgggc
    24241 gacggcctcg tcggtggcgt ggccacggtg gacgggcacc cggtggccgt cgcgtcctac
    24301 gactacacgg tgctggccgg cacccagggc ctgaacaacc accgcaagaa ggaccggctc
    24361 ttcgaggtgg ccgagcacaa ccggttgccc gtcgtgttct tcaccgaggg cggcggcggc
    24421 cgtccgggcg acacagacgg caccggcatc gccggcctgg actgcctcgc cttccactac
    24481 ttcggcaagc tctccggcct cgtcccgctg gtcggcgtcg cgagcgggcg ctgcttcgcc
    24541 ggcaacgccg caatcctcgg ctgctgcgac gtcgtcatcg ccacccgcga ctccaacatc
    24601 ggcatgggcg ggccggcgat gatcgagggc ggcggcctcg gcgtgtaccg gcccgaggag
    24661 gtcggcccga tcgaggacca gatcgcgtcc ggcgtggtcg acgtggccgt cgaggacgag
    24721 gtcgaggcca tcgccgtcgc caagcagtac ctgtcgtact tcaccgagcc ggacctgggc
    24781 gtgtgggact gcgccgacca gcggtggctg cgccgggcga tcccggagaa ccgcctgcgc
    24841 gtctacgagg tgcgcggggt catcgagacc ctgttcgacg ccgactcggt gctcgagctg
    24901 cgccgcggct tcggacacgg catggtcacg gcgctggccc gcatcgaggg ccgggcggtg
    24961 ggcgtcatcg ccaacgaccc cgcccacctc gccggcgcca tcgacagcga cgccgccgac
    25021 aaggccgcac ggttcatgca gctgtgcgat gcccacgacc tgcccatcgt cttcctgtgc
    25081 gacacgcccg ggatcatggt cggccccgac gccgagcgca ccggcctcgt ccgccacgcc
    25141 agccggctgt tcgtcaccgc cggcgggctc accgtgccgt acatgaccgt ggtgctgcgc
    25201 aaggggtacg gcctcggcgc ccaggcgatg gcgggcgggt ccttccacgc tccgctgttc
    25261 gtggtcggct ggccgaccag cgagttcggc ggcatgggcc tggagggcgc ggtgaagctc
    25321 ggctaccgca acgagctcgc cgccatcgag gaccccgagg agcgacgccg cacgttcgac
    25381 gagatggtcg agcggatgta cgaggtgggc aagggcacct ccatggccgc ggcgttcgag
    25441 atcgacgacg tgatcgaccc ggtggacacc cgccgctgga tcaccaccgg cctggccgcc
    25501 gcgcccgcgc cggcgccgcg caccggcaag aagcgaccgc acatcgacac ctggtgaggc
    25561 agaccccccg atgaccaccc tcgacatccc cttcgaccgc tacccgcgct tcgacgagct
    25621 cagcgagcac ctgcagcgcc tcgcagccga gtaccccgac ctgctcgagc tcgacgtgct
    25681 cggccagagc cacgagggac gtgagctgtg gatcgccacg gtcacgaaca ccgccaccgg
    25741 cccccatcac gagaagccgg cggtctggct cgacggcaac atccacgcca gcgaggtcac
    25801 cgcatcggtc gcgctgctgc acctgctcac ccacctgtgc accgggcacg gcaccgacca
    25861 gcgcgtcacc cgtgccctcg acacccgcac cttctacgtg gtgccgcggg tcaaccccga
    25921 cggtgccgag ctggcgctgt ccgaagtgcc cttcgtggtc cgttcgacca cccgggcctg
    25981 gccccggccc gagcagcgcg aggggctggt cagcggcgac gtcgaccacg acggacgcag
    26041 cctgcagatg cggatcgagg atccgaacgg cacgtggaag gcgcacccgt cgatcccgaa
    26101 gctcctggtc gcccgcgagc cggacgagga cggtcccggc ccgtactacc ggctgctgcg
    26161 ggaagggcac gtcgagggct acgacggggt gaacatcccg accgctccgc ccctcgccgg
    26221 catcgactcg aaccggaact tcccgtacca gtggcgccgc ttccccgagg gcgagggctt
    26281 cgggtggggc cacggcgact tcccgaccag cgagcccgag gtgcgcgccg tcgtccaggg
    26341 tgtcgtggac cgcccgaaca tcggcatcta cttcgcccac cacacgtact cgggcgtgat
    26401 cctgcgcccg tactccgatc ggcccgacga cgcgttcccc acggtcgaca agtggaccta
    26461 cgacgagctg ggccgccgag ccgaggagat caccggctac ccgtacctcg gcgtctacga
    26521 cgggttccgc tacgacccga aggacgtgat cacgggcgtc ggtgacgact gggcctacga
    26581 ccaccggggt ctgttcgcct ggaccaccga gttctggaac ccgctgaccg ccgccggcat
    26641 cgaggacccg cacccgatcg agtggtaccg ggaccacacc ctcgacgacg agctgaagct
    26701 gctggcgtgg gtggaggaga acgtccccga cggctacgtc gactggtacc ccttcgagca
    26761 cccgcagctc ggctcggtgg agctcggcgg ttggcacgcg gcagcggcgt tccggaaccc
    26821 gccgccccac ctgctcgagg ccgaggtcga accccactcc gagctcgccg tgttccaggc
    26881 gctgtgcctg cccgagctgc gcgtgcgcga cacgtcggtc gagcggctgg gcgacgacac
    26941 gtggcaggtg cgcgtggtcg tcgagaacgc cggctggctc ccgaccaacg tgaccaagcg
    27001 ggccgaggag gccaaggtga cgaccccgat cgaggcgcgc ctggtgctgc ccgagggcgc
    27061 ctcgctggtc cacgggacgg cgcaggccga gctcggccag ctgtcgggac gcgccctgcg
    27121 gcgatcggcg gtcggcatgt tcggtcctcc caacgacggc acgggcgatc gggcgtcggc
    27181 gacgtggacg gtccgcgcgc ccgccggcac cagcctcgag gtcgtcgtgc gcagcgaccg
    27241 cgccggcgtc gtgcgggcga ccgtggagct cgccatcgac tgacgggcct gagctcgaga
    27301 tcgccccggc catcgtcacc ggcctcttct acgccatgct cgccatcgtg gtcggctccg
    27361 ccgtcatcgc catcggcggc gccggcgtga ccgagatgcg gccctaccag gcccggcagc
    27421 gtcgcgcccg ggggtcggtg gcggagctca ggtggccggg tacccgtcgc gctggagcag
    27481 ccgggccagg tgggcggcgt tggctgccgc ggtcttgttc gtggccgcgg tgacgtccgg
    27541 cgtgggctcc ttgtccttgt agtcgatgcc gtgcatggcc tcgccgaccc agtaggtgac
    27601 gccgccggcg gggatcgtgt agccgacgtc gttcagcgcc tggaacagct cggccgagac
    27661 gtggtgggcg ccgtcctcgt tgccgacgac ggccgcgatg gccaccttgt cgtagctgat
    27721 catccggcct cggtcgtcct cctcgccgag gaaggcgtcc atgcgctcga gcacgcgctt
    27781 gacgaccgat gacggctggc cgagccagat cggggtgccg acgacgagga tgtcggcgtc
    27841 gaggatctgg cgtcgcagcg ccggccattc gtcgccgtct ccttcgtcag acgtgacgcc
    27901 gggcttgatg tcgaggtcgg cgacccgcac gatcggcgcg gtctcgacat cgaggctgcg
    27961 gagctcgtcg aggacctgcg tgagcatgag ctcggtgctc gactcggcgt cgccgggctt
    28021 gagggtgcag ttgagggcga tggatcggag ggtcatgcct ccacgtctat cgccggatcg
    28081 aggcgatcct ccggccggcg ttcagtgggt ggcggtcccg aggtggtggc cggggttgcg
    28141 gcccaggtgg tcggcgacgg ggacccgcgc ctcggactcg tagccgacgg gcagccagac
    28201 gtcgcccatc gtggacaggt agacgatctc ccactggtgg tagccgcggt aggtgttcgg
    28261 gtcctgctcc atgaccgagg cgtagtgctt cacgcctcgc acgtagcgat cgctgtggtt
    28321 gtagttccac agggcgttgt cgatctcgcc gcgcccgccc ccgttgtggg cgaggtagcg
    28381 ggcggcgccc atgatcgcgt cccggtagtc gttcacgtca ccgccgtccc cgaaggccgc
    28441 ccaggtggcc ggcatgaact gcatcgggcc ctgcgcgccg gcgaccgaga cgccgcggat
    28501 ccggcccatg ccggtctcga ccaggttcac ggcggcgagg atctcccacg gcacaccgaa
    28561 cgaggcctcg gcctcctggt agtacgacag cagctcgtcg gccgacgggg gttcgacgat
    28621 gcgccacgcc ggcagggcgt cggagagcgt gcggtgcatg ctgcggaact cccggcgcgc
    28681 cgccacgttg gccgcagcag cggcgctgta gggctcggac agcgacgcgg tcacctcggg
    28741 ctcccactcc ggcgtgcggg ccagctggcg gtacagcagc tgctggtcga acgcggcccg
    28801 atcgagctcg gcggccggtg tcgccgggtc gcggacggcg tcttcggcgc gcaccaggcg
    28861 ctcggtcagc tcggccgggg aggccgccgg tgcgggggcg gtgtcgggcg ccggcggtgc
    28921 cgtcgtggtg gtggtggcca ccgtcgtggt ggtggtcgtc ggagcttcgg tggtggtcgg
    28981 cgccggctcc gtcacctcgg tggtgtcgtc gctgctgcgc tccacgtcgc tggcgtcgct
    29041 gccgcaggcg gccaagacga ggctcgtcgc cgccgcgagc cccaacaggc gtcgtcgtcg
    29101 catgcgccca cggtaggggt cttccggcgc agttcctcgg cgccggcccc ccgtcagacg
    29161 cggaaggcca gcatggcggt gtcgtcgctg acgccgtcgc ccagcatggc cgcggccagg
    29221 ccgtcgagga tgtcgtcgac ggtgccgtcg agccgggtgg cggcctcgcg gaggcgctcc
    29281 agccccacct cgatgctctc gccgcgacgt tcgtagagcc cgtcggtgaa cagcaccacg
    29341 acgctgccgt ggggcaacgt ggtccgcttg accgggaacg tcgggtcgtc gaggaagccg
    29401 atcggcggcc ccgggctgac gcgcaggaac cgggtctcct cggggctgat cagcagcggc
    29461 gcgggatggc cggcggtggc gaagcagatg gtgcgggcca cggggtcgac gttgccgcac
    29521 agcatggtga cgaagtcgcc gcggatgtgc tcgagctggt tgacagcccg caggatctcg
    29581 ccgggctcgg tgctctgccc ggccacggcg tgggtggcgt agcgcatggc cgccatgacc
    29641 gcagcggcct tgacgccgcg gccgacgacg tcgccgacga cgaccgtgaa ggtcccgtcc
    29701 tcgtcgttga tgacgtcgta ccagtcgccg ccgatgtcgg tgccctcggc tcccgggaag
    29761 tagcgagcgg cgacctcgat gccctggagc cggtcgaggt gcgtgggcag caggctgtgc
    29821 tggagggtgt gggcgatggc gtgctcgcgc tggtagagct cgtggagctc ctcggtgaag
    29881 gtctcggcgt cgagccggcg gcgatgcagg ctctcgacca gcacggccgc gatgccgccg
    29941 gacacgaggc cggcgagcgc ggccagccac ggcaggacgg cgaggacccc gccgcccagg
    30001 gtgccggcgg gtgtcacgac gaagagcagc tgggtgtccc cgaagggcac ctcccggctg
    30061 gtccggcgtc cgtcgatcgg gaggtccggg gtggtggagg tcaggaggtc ctcgtcacgc
    30121 tcgccctcac cgaggtagat ggcgagatcg atgccgtcga aggcctcgcc gtcgcggtcg
    30181 tcgctgatgc ggggtccgtc caggcgcacc tcggcgatga ccaccaggtc gctggcgccg
    30241 gtgtcggtgc cgtaggcgag tcggcggtcg tcgtcgtcgt cgaggaaggc gctgacatgg
    30301 accaggtccg ggtccgggcg gacctcctgg agcgtggcga ccaggtcgtc gtcgtcgagc
    30361 acggtcgggg caccgagctc caccacgggt tcggcaccgg tccggcgcag gacggtgagc
    30421 gtcgtgaacg actcctcatt gatgtcaccg gcgagctccg cctccatcgc ctccgtgtcg
    30481 ccgtccagca ggtcgaccac ccgagcgacc tgcgcgacgt tggagaggat tcggggcacc
    30541 gacgcctgca ggaccgccgt cgcctcctcc gcctgctggg ccaggaggcg gtcctcgttg
    30601 gtgtcgtggg cccgctgcgc cgctgccgtg agggccaccg tgatcgcgag cgcgatgacg
    30661 accacgacga gggtggcgcg accgatgcgg gggaggcctg atggcacggc gggggctgct
    30721 cttcggttgg tgcggcggga gggctacgag gccgggtcga ggcgacggct gcagcgcacc
    30781 tgcacggcgc ccccggtgct gtcgacggcg agggagtcgg tgtaggcgcg cacgatcatg
    30841 aggccgcgcc caccgccccg gagcggatca tcgaggtccc agtggcggat gtcggccacg
    30901 ccgtcgggca ggcggttgga caccacgagg tggagcatgt ggtcgtcgat ctcggctcgg
    30961 acctcggcgt cgggttcgtc cggcggcgcg ccgtcgaggg cgttggagac cagctcgctg
    31021 acgaccacgg acatgtcctc gagcagttcg gcatcggtcg tggtctggcg cagccaggag
    31081 tcgaaggcct cgcgcgccga gatgatcgat cccgaaccgc tgccgaggcg ccacgcgaac
    31141 tcgccctgcg tccgcccggt cgcgctgttt ccatgcactc ggatcgccct cctcccggcc
    31201 ggaacgggac caccctccca ggggaccctt cgaccagcg
//
LOCUS       DATISU010000390        23712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_777454, whole genome shotgun sequence.
ACCESSION   DATISU010000390 DATISU010000000
VERSION     DATISU010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 23712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23712
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777454"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VLR27_15415"
     CDS             complement(<1..933)
                     /locus_tag="VLR27_15415"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HSP04898.1"
                     /translation="MLNRRWMRLLALLLGLSLIAAACGDDDDTDTATGDTEDATEDTA
                     TEGETEGGEASDSDFLACEVTDTGGVDDRSFNENAYNGLVQAAEEIGFEPVVLESQSD
                     ADFDPNIQALIDQNCDLVVTVGFLLGDATEAAAQENTDTDFAIVDYAYETEYENVLGL
                     TYATDQAAFMAGYVAASQTQTGTVGTYGGLNIPTVNIFMKGFEAGIEHHNEQKGTDVQ
                     LVGWSTADDDGLFTGDFEDLSKGRQTTESLLDQGADIILPVAGPVGQGSVEAIRAGGD
                     DAKLIWVDTDGCESVADACDLFLTSVMKNFDVSVY"
     regulatory      1104..1218
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1206..1883
                     /locus_tag="VLR27_15420"
     CDS             1206..1883
                     /locus_tag="VLR27_15420"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HSP04899.1"
                     /translation="MDLDRPPDIVVVAGGEPVAPGLVAAVPGTARIVAADAGVDLARS
                     LGWEVHVAVGDFDSVSAEGRSALADEGADVRVHPMAKDATDLELALEVAAELAPARPA
                     PGHRVLVIGLEGGRPDHALANLLLAAAEPFAALDIELVLAQGRAWVVRDHLTGSLPAG
                     GLVSLVPVHGPATVSVSGVRWPLDRAALAAGTTRGVSNEAVGGPFELRVHAGAVLCIT
                     PRTEETP"
     gene            1880..2959
                     /locus_tag="VLR27_15425"
     CDS             1880..2959
                     /locus_tag="VLR27_15425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HSP04900.1"
                     /translation="MIPIRRPLSALLAATLVVAACGDDDAETTPEPDGGGAGATSVTL
                     LTHDSFDVSDEVLAAFTEDTGITVELLPSGDAGSMLNQAILTKGSPQGDVLFGVDSTF
                     LSRALDEDLFVAYESPALEGVDESLVLDPEHRVTPIDTGDVCLNYDKAGLAAAGLEPP
                     EDLADLADPAYAGTLVVENPATSSPGLAYLLATVAEFGEDGWQDHWRALRANDVEVAA
                     GWEEAYYGSFSGAAGSPGDRPIVVSYASSPPAEVIFAEEPLDDAPTGVVDGSCYRQIE
                     FAGILAGTEHEAAAQQLIDFLLSPTFQEDVPLNMFVYPVIDVELPAEFVEHGADPEDP
                     YLLDADVVAANRDAWIEEWTEIVLR"
     gene            2967..4640
                     /locus_tag="VLR27_15430"
     CDS             2967..4640
                     /locus_tag="VLR27_15430"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HSP04901.1"
                     /translation="MSSAERAPRASRAVTAAAFAVPVAFLAVFFAVPVATLITTGLRE
                     GGRWELGAVGDVLTSASTRSVAWFTLWQAVLSTAATVVAALPAAWLIGRVRFPGRSLF
                     VAALIVPFVLPTVVVGTAFLAVLGPDGPVAALGGVLGADVDLRRTATAVILAHVFFNY
                     AVVARTVGAAWMAVDPAAEDAARTLGADRWTTFRTITLPQLRPALAGSAAIVFLFTFT
                     SFGVVKILGGPRIATIEVEIHRLTTQLLDLRTASVLSLLQLAAVIAALALYRRAGVRR
                     GSARTRAGRSVLHPPAGAERWVLAATISSAALLVGVPIATLVWRSVDTAGGPGLGYYR
                     ALGSTGRGSALFVPPAEAVRNSLLIGAAAAATALVVGGLAAAGLHRLGGRARGIADSS
                     LMLPLGVSAVTVGFGFLIALDEPPLDLRTSPVLIPAAQAVIAIPFVVRTLLPALDAVP
                     GRLREAAAALGAPPHRVWWEVDARLLRRPALGALGFAYAISLGEFGATVFIARADAPT
                     VPVAIFRALSQPGALNVGQAMALSTILLVLTAAVMLLVDRVGDARVGGF"
     gene            4642..5655
                     /locus_tag="VLR27_15435"
     CDS             4642..5655
                     /locus_tag="VLR27_15435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP04902.1"
                     /translation="MSSLIVRGVSKAFGDLAVLREVDLAVEDGTTLALLGPSGSGKST
                     LLRIVAGLEQPDAGSVHLGDADVTGRPAHRRGVGVVFQDQALFPHLDVAGNVGFGLRM
                     AGVDRRERAERVRDALGLVGLSGYERRDPATLSGGEAQRVALARALAPRPAVVLLDEP
                     LAALDQLLRERLLVELRALFDDLDVTVVAVTHDPTEAAALGDRIAVLLDGRIAQVGTP
                     GAVIAAPSSAAVAGLVGQRNVLAGRVVPGGVETPLGLLPVDPGRSRGEVDVLVPPSAL
                     VPTAQGRVRSVVRGLLYRSGTPVAVVDAGGVALDVLAEPGWVVGDEVALRIVPDSVHL
                     MGR"
     gene            5722..6153
                     /locus_tag="VLR27_15440"
     CDS             5722..6153
                     /locus_tag="VLR27_15440"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HSP04903.1"
                     /translation="MRRLTMILAALTLVVAACGDDDDGSAAPADDEAVTAPTTAPAGL
                     GAEDETDDGTSPSGEAEVSISDFSFQPDRIDVGAGATVTWTNDDGVPHTVTAGTADAV
                     GDTFDEPVEAGQTAEVTFDEAGTYDYFCAIHPTMTGQVVVS"
     gene            6159..6671
                     /locus_tag="VLR27_15445"
     CDS             6159..6671
                     /locus_tag="VLR27_15445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP04904.1"
                     /translation="MAHADLDAAVRHLHAAHAPALHAWARHRTDDAREAEEIVQETLI
                     LAWRKQDQYDPARGSERAWLFGIARHVAVDRHRRQQRHLQVVPGADAPEPVDDGEIDR
                     AVEGSHIRDALWALSDAHRQVVIETYYRGRSVREAAAALEIPEGTVKSRLYHALRALR
                     RELERREVLP"
     gene            6668..7324
                     /locus_tag="VLR27_15450"
     CDS             6668..7324
                     /locus_tag="VLR27_15450"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSP04905.1"
                     /translation="MTASDHGRYRDLTAAYVLGALDAGERVEIEQHLEACEACRSDVV
                     SFAPLPALLSRVDADDAASDHPGAGADALVAAVRADVDQLDRSRRRWRRGAGLAAAAA
                     LVLAAFVVVRDDDPARWSDGVELAVEAISAETTADVVATERGWGTYLSLSAEGLPPRD
                     AYEMWTVDDEGAWEPVGSWGPTPEGRADLGCSSALPLGAIDRVVVTSDDRGDELLVAR
                     "
     gene            complement(7332..7748)
                     /locus_tag="VLR27_15455"
     CDS             complement(7332..7748)
                     /locus_tag="VLR27_15455"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP04906.1"
                     /translation="MEPRISVITLGVEDADRARRFYEALGWSGESPDGEVVFFQAGGM
                     VLALWSREKLAHDSEVTDPGGWGGVTLAHNVGSPEEVDAVLAQAEAAGGTIARPGGKA
                     FWGGYTGIFIDPDGHPWEVAHNPIWRLDDDGTVHLR"
     gene            complement(7775..9274)
                     /locus_tag="VLR27_15460"
     CDS             complement(7775..9274)
                     /locus_tag="VLR27_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04907.1"
                     /translation="MLRRLLAVGAFGLVSVSSCGAPATDLPWAAEAVVYFEAMEETGA
                     RSITEQVRRFYDADALVDTWYTSGSACEGGPACEEWLTDRFGATTDETTTGMLFLDPR
                     GAVRMVNRSWTSGVASELVVQDIGPEGLVTRELQPRSVDDVRAVLGPAATASLEELAA
                     AHLEVWSAADPAAVAARYAPDAELVDGLVGLQLTGRDAIASHALAKRFAAPSATPPAV
                     LLDVDGRGAMYVYLPDALPAGPSVPYQLFFIQTLGGPARCPGTTVVALTVRDGLVGHE
                     RRYHEIGATRRCADDLTPPEGWWTGLVVPSLPVEVTGTVVVAGVQVTVHDGTPALERY
                     VRWGLERFVAGGLTVPHVALVGFDEDLHPDECPNERDGCCIEDERGSAIYICATEDDI
                     CTDPGCQAVSVRWRQLLLHELAHAWMSDHVDLDAQEQFAALRGLTAWDDPATSWDRRG
                     VEHGAEVLAWGLMDEPVVPYRLGASSCDELAAGFRLLTGTAPPRGCPTG"
     gene            complement(9375..10163)
                     /locus_tag="VLR27_15465"
     CDS             complement(9375..10163)
                     /locus_tag="VLR27_15465"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HSP04908.1"
                     /translation="MSDPVVLVERNDAVATVTLNRPGARNALSRELRKSLWQAMSELE
                     ADVSVAAIVLTGADPAFCAGLDLKELASTSGAALGETGATGDTATGRGPIPPMETPVI
                     GAVNGVAITGGFEVALACDFLVASDRAAFADTHARMGIMPGWGLTVLLPEAVGFRRAK
                     ELSTTGNFLDASTALAWGLVNHVVPHDELVPFAQQLAADIATNDRAGVRQMLQTYEQG
                     ALITRGEAWALEGRVAGEWQAGGRDGADLEARRKAVTERGRSQV"
     gene            complement(10200..10790)
                     /locus_tag="VLR27_15470"
     CDS             complement(10200..10790)
                     /locus_tag="VLR27_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04909.1"
                     /translation="MLDHVFTDAIGALRDALETAMLERMTAEERFQVDVLLGDTTWET
                     SYGLPGEGNPPRVRADITLDWPTWSQSAFRSWLRNEEPEDDPRIEISIVLRLQRLVEL
                     PSPDQVTAVLPAESPSVGPDQLHRTAPTVETAYTAELEPVSHALELAYEGAYELDDKA
                     LGDGAILDDHFSALGGWIASMLVRLGDLPLAYHDPY"
     gene            complement(10896..12878)
                     /locus_tag="VLR27_15475"
     CDS             complement(10896..12878)
                     /locus_tag="VLR27_15475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HSP04910.1"
                     /translation="MLTASGLSLAHGSRVLFRDVTLSLSAGRRVAMVGGNGQGKTSLL
                     SILVGDAEPDSGTVTKPKDMRIGYLAQEIPSEDDGTVLDTVLGGRPDIMRLHHQIVEG
                     PADASPEALHRFGEAQTQYEQLNGYALEAEAHTVLAGLGFAPDDADRQVSELSGGWRM
                     RVALARLLLSAPDLLVLDEPTNHLDVDSVGWLEGRLRAWTGALLFVSHDRDFIDNVAT
                     HVVELANETLTEYVGGFAEFVVQREERIAQLEAQAASQAKARAQTERFVERFRYKATK
                     ARQVQSRIKMLEKLETIEIPDRKELQARFQFPEPPRTSRVVAELSHMTAGYGDTPILR
                     DVSVHVERGQKIALVGPNGAGKTTLLKMMLGDLPPMTGTAEIGQSVTVARFEQHQADE
                     LAPDQTVLQVARDGIGSDTPRGKTLRTYLASFGFRDDAVERQVGMLSGGERTRLALAK
                     AMAEPAGLLILDEPTNHLDLASCDMLEDALKVYPGTVLLVTHDRYLIRSVADDLIEVR
                     NGTAKFHHGVDEAVLTPTTATTRRDASGSPVKSAKKSGGGRSGKAAKAKGPTQPAKAP
                     GQSSPTSPKQKQKQGGGGSQQDRDLRKQVNRLEKQWETAEAKVAELQAALGNPDVYAD
                     ADKLTELTTALDAAKDEAVRLMTDWEQAASRLERSS"
     gene            12963..13910
                     /locus_tag="VLR27_15480"
     CDS             12963..13910
                     /locus_tag="VLR27_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP04911.1"
                     /translation="MADGPLDPDALVRARGLTKRFDDFVAVDGIDFEVRRGEVFGFLG
                     PNGAGKTSTMRMISCVSPATGGELSVLHLDPSSDGPAIRSRIGVVPQEDNLDTELSVW
                     DNFIVYGRYFGLPRRVLRERAQELLEFARLTDRRDDRVDPLSGGMKRRLTIARSLIND
                     PDLVLLDEPTTGLDPQARHLLWDRLYGLKRRGVTLIITTHYMDEAEQLCDRLVVMDQG
                     RIVDEGSPRQLIDRHVTPEVIELRFAEPAEQEAALSSAGEAAERIEVLADRLLLYTRD
                     AEATHRALAERSVEPLTELTRRASLEDVFLALTGRTLEE"
     gene            13921..14691
                     /locus_tag="VLR27_15485"
     CDS             13921..14691
                     /locus_tag="VLR27_15485"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP04912.1"
                     /translation="MTQSVWRALERELLVFVRTWRGSAFTAIVQPVLFLLAMGIGLGG
                     LVDEGAGSASVTLDYLDFVAPGLLAASALLAVAGEALWGVMGGVKWMGQFKGMVATPM
                     TPGDVYGGLVLSAGVRAGAAAIAFLVVAAVLGGVSSLWAPLAILAAVLLAIVTMSALS
                     AYAVRKEDDRTFPLIMRLGVIPMFLFSGTFFPVEQLPDLVQPIAVFSPLWHGVEVARD
                     AMTGAVDGWTLLHAGVLVALTAVVLPIGVRGFERRLTP"
     gene            14688..15500
                     /locus_tag="VLR27_15490"
     CDS             14688..15500
                     /locus_tag="VLR27_15490"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP04913.1"
                     /translation="MSVQDLVDHGRIAPPLDPRKRFLRLTERNVVGHLKAWPILLTGV
                     LEPFLYLLSIGIGVGALVGDITYAGRQVPYETFVASGMLATAAMNGAVFDTTFNFFIR
                     LRYAKLYDAVLATPLEPRDVALGEVTWALMRGTFYSAAFLVTMALFGLTESWWAIAAL
                     PATVLIGAAFASIGLAGATYLRSFLDFDFVNLAITPMFLFSATFFPLSQYPDGLAAVI
                     RFTPLYQGVALCRALTTGEVGIGLLVHVAYLLGMVLVGVAVASRRIDGLLRS"
     gene            15582..16244
                     /locus_tag="VLR27_15495"
     CDS             15582..16244
                     /locus_tag="VLR27_15495"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSP04914.1"
                     /translation="MIHEIDIDLPVRTVYDQWTQFEDFPLFMKHVTDVRQVDDTHTHW
                     KAKISGVSREWEAEITEQTPDQRVAWTATDGTANSGVVTFHALDDNTTRVVLQMETDP
                     KGFLEKVADWGGYISDRAKKDLDQFKDFIEARGKSTGAWRGQVERDSMRDLHSREDEL
                     RELSDHELAKRAEDAGLERPLDRNRDWLVSALARDGYDRNTSRDHDGDDDRRDSEDAH
                     TS"
     gene            complement(16267..16899)
                     /locus_tag="VLR27_15500"
     CDS             complement(16267..16899)
                     /locus_tag="VLR27_15500"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP04915.1"
                     /translation="MTVWLDALEVGDEPAAWASAGFAVDGSEVAIGTVRVVCTGDGAG
                     ADRWRLRADDEDVPRSVDGIATSVTDRPPPAPVEHPNGVVRIDHIVLRSPDLDRTTEA
                     FGALGLDLRRTRDVGSGDRAMQQRFFRMGEVILELVGPPSATGDGPCSIWGLALVTPD
                     IDASVAQLGEACSPSKPAVQPGRRIATVRTRELGIGPTIALMTPHERATA"
     gene            17057..17815
                     /locus_tag="VLR27_15505"
     CDS             17057..17815
                     /locus_tag="VLR27_15505"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSP04916.1"
                     /translation="MTMSETPVSPATVSALMRLTADPDPAETDLAGVVAADTGLAARV
                     LAVANSASFGLSRQVTQVHQAVGLVGMNLVQTLAIAGSCQLLDGTVGLPRARRHAVEV
                     ACAARALAERAGLDGGAAFAAGLLHDLGEILLWRDDPEPYAEAHARWSDPTTQLREER
                     GAYGTDHALVAREQLAAWNVPGNIVDAVGDHHRPDLLHRDICTVVVAAEELTDRDVDW
                     SPRFEIFGVTGAALPSLRDEVSTQTDELLSLLVR"
     gene            complement(17816..19516)
                     /gene="leuA"
                     /locus_tag="VLR27_15510"
     CDS             complement(17816..19516)
                     /gene="leuA"
                     /locus_tag="VLR27_15510"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HSP04917.1"
                     /translation="MGTEPRNGKPGTMPFEKYQPFLPLELPDRTWPDQRIEAAPLWCS
                     VDLRDGNQALIDPMDPVRKRKMFDLLVGMGFKEIEIGFPSASQPDFDFCRHLIEEDLI
                     PDDVWIQVLVQSRPELIERTYEAIAGAPRAIVHFYNSTSVLQRRVVFGLEKDGITDIA
                     VNAAKLARKLEDRVPDTHVRYEYSPESFTGTETDYAIEICDAVREAIDPGTDDKLIMN
                     LPATVEMYTPNIYGDVIEYFHRNIPHRDQVVLSLHPHNDRGCAVAAAEFGVMAGADRV
                     EGTLFGNGERTGNVDLVTLGMNLFVQGVDPEIDFRDIDAVRRTTEYCNRLEVHPRHPY
                     GGDLVYTAFSGSHQDAIKKGLAALAETIPEGGAAGDYPVWEVPYLPIDPKHVGRTYEA
                     VIRVNSQSGKGGVAYVMKEEHGFDLPRRLQIEFSKAIQGVVEDTGTEINPGEMWAQFE
                     GEYLGIGRYRLDSYEMRTSDEAAEITAQVIADGEHHTVIGKGNGPVSAFVNGLREHLG
                     VDVDVLDYTEHAIGAGADTQAVAYVETQTPEGLVRWGVGVHDSIVVASLRAVLSAVNR
                     "
     gene            complement(19712..20677)
                     /locus_tag="VLR27_15515"
     CDS             complement(19712..20677)
                     /locus_tag="VLR27_15515"
                     /inference="COORDINATES: protein motif:HMM:NF021832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine salvage family protein"
                     /protein_id="HSP04918.1"
                     /translation="MADDDAPLPDRVRTGAAALAGRAELVRIDDEALDRLAASLDPTA
                     PEPFPEERWEGSVADRAMGVVAWNAVNFGSGWFPHVVKLPGHSGARTMGARWQQHCRT
                     AGVPGAEWLADVTVDEVAGVFHQPLEGEVRGLLDAFTAAWRELGVHLLEHHGGSAAAM
                     VGAAGNSGASLAAELAALPCWDDRHRLDDLEVPLHKRAQIVVSNLAGALADDELGRFD
                     DLATLTAFADNLVPHVLKMAGVLVVDPALDARIEREELLEAGERGEVELRALGVHAVE
                     LLVDRLRSAGHDEVTAATLDHRLWQQGQDPAVKARPRHRCRCTYY"
     gene            20692..21114
                     /locus_tag="VLR27_15520"
     CDS             20692..21114
                     /locus_tag="VLR27_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP04919.1"
                     /translation="MPELPRFHLAVPVDDLAAAEAFYGGVLGCPTGRRSDQWIDWDLH
                     GHQLVTHLVETRPGADDTNPVDGQDVPVPHFGLLLEVDAFHAMAERLRAGEVPFVIEP
                     YLRFEGLPGEQWTLFVRDPAGNAIELKAFADDADVFRS"
     gene            21215..23104
                     /locus_tag="VLR27_15525"
     CDS             21215..23104
                     /locus_tag="VLR27_15525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HSP04920.1"
                     /translation="MLRILRSRPRSSLAVAAIALLASAVAISPIADAHPDPLEGGHKR
                     QAAGGIVVQVNSPDGLAGDLPAVQWGDTGEIDGQTAELAWAGSGCSPLSYGTADVAGK
                     IALIESAAADDPCPASTFFQRVQSAEQAGAIGLVAIPAEGAEPTGSATAVTATIPALE
                     VFRSPEVLAVRDAVLAGEAVEVTLADTREPLPRIIDEPCVDGMAGPFPCDGVDLLSFV
                     PQEEFDGAGVSDIWGWTDQETGGEYVMFGKTNGVALYNVTDPYAPIYLGEIPNEAALQ
                     EIWHDIKVFQDHAFIVSESMPHGMTVFDLTQLRGVTEPQVWKPTARYDGVMSAHNVAI
                     NEETGFAYIVGGNAALVLPDQCLSGLHMVDINDPTNPTFAGCYPLDGGPGTAARTAGL
                     PVGAAYVHDTQCVVYRGPDQDHFERELCFNAAEDKITIVDVTDKLLPSTVATIDYEAV
                     AYAHQGWLTEDHRYLLTNDELDEQADPGTRTRTIVLDVTDLDAPVVHDIHLHDTISID
                     HNNYVHEGLAYQSNYTTGLRIFDVATLDDVDPRLDPVGFFDTYPFSDDPTFDGTWSNY
                     PYFGSGTIAVSGIGEGLFLVKLADAGTDTDTGDGDGGEEPVPGSNGNGRPDHAGPKDG
                     RGPKG"
     gene            complement(23124..>23712)
                     /locus_tag="VLR27_15530"
     CDS             complement(23124..>23712)
                     /locus_tag="VLR27_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HSP04921.1"
                     /translation="LDVIDELNGRWDDFRILTGIEVDILADGTLDQDEDLLDRLDVVV
                     ASVHSKLSMDAKEMTRRMVMAVASPHVDILGHCTGQKVPGHQRQIGKSDFAGRIGSSF
                     DADVVFAACARFDTAVEINCRPERQDPPDHLLELALEWGCKLAIDTDAHAPGQLEWQP
                     FGCDKAARHGVAPEQVVNTWAADQLVEWATAHPTS"
BASE COUNT         3118 a         8613 c         8454 g         3527 t
ORIGIN      
        1 gtagaccgac acgtcgaagt tcttcatgac gctggtcagg aacaggtcgc aggcgtcggc
       61 gacggactcg cagccgtcgg tgtcgaccca gatcagcttg gcgtcgtcgc cgccggcgcg
      121 gatcgcctcg acggagccct ggcccacggg gccggccacc ggcaggatga tgtcggcgcc
      181 ctggtcgagc agcgactccg tggtctgacg gcccttcgag aggtcctcga agtcgccggt
      241 gaacaggccg tcgtcgtcgg cggtgctcca accgacgagc tgcacgtcgg tgcccttctg
      301 ctcgttgtgg tgctcgatgc cggcctcgaa gcccttcatg aagatgttca cggtggggat
      361 gttgaggccg ccgtaggtgc cgacggtgcc ggtctgggtc tgcgacgccg cgacgtagcc
      421 ggccatgaag gccgcctggt cggtggcgta ggtcaggccg aggacgttct cgtactcggt
      481 ctcgtacgcg tagtcgacga tggcgaagtc ggtgtcggtg ttctcctggg cagccgcctc
      541 ggtggcgtcg cccagcagga agccgaccgt cacgacgagg tcgcagttct ggtcgatgag
      601 ggcctggatg ttggggtcga agtccgcgtc gctctgggac tcgagcacga ccggctcgaa
      661 gccgatctcc tcggcggcct ggacgaggcc gttgtaggcg ttctcgttga aggaccggtc
      721 gtcgacgcca ccggtgtcgg tgacctcgca ggccaggaag tcactgtcgg aagcctcgcc
      781 gccctcggtc tcgccttcgg tggcggtgtc ctcggtcgcg tcttcggtgt cgccagtggc
      841 ggtgtcggtg tcgtcgtcgt cgccgcatgc agcagcgatc agggacaggc cgagcagcag
      901 cgcgagcagc cgcatccatc gtcggttcag cacaggggtt ctacctccgg atggggtcgc
      961 gcacggatcg tccgcgtacc cgtcaagtgt catggggacg gcgcaacggt aggtgtccgg
     1021 gaccccgagg acaatgatcc agccgcgtct cgtgaccaag gtcccggtgg accggtccgg
     1081 tatggtcacg gtggcgccgc acaagaaccg gcgcggggag ctcggggcac accgggctga
     1141 gagggtgtct gcaggccgtc accgaccctg gaacctgatc tgggtaatgc cagcggaggg
     1201 aactcgtgga cctcgatcgt ccacccgaca tcgtcgtggt cgccggaggc gaaccggtcg
     1261 cgcccggact cgtggcggcg gtccccggca cggcccgcat cgtcgcggcc gacgccggcg
     1321 tcgacctggc ccgctccctg gggtgggagg tccacgtcgc ggtgggggac ttcgactcgg
     1381 tgtccgccga gggccggtcg gcgctcgccg acgagggggc cgacgtccgc gtgcacccga
     1441 tggccaagga cgccaccgac ctcgagctcg ccctggaggt cgccgccgag ctcgcgccgg
     1501 ctcggcccgc gcccggtcat cgggtgctcg tgatcggcct cgagggcggg cgccccgacc
     1561 acgcgctcgc caacctgctc ctcgccgccg ccgaaccctt cgctgccctc gacatcgagc
     1621 tcgtgctcgc ccagggccgg gcgtgggtgg tccgagacca cctcaccggc tcgctgccgg
     1681 cgggcgggct cgtgtcgctc gtgcccgtcc acggcccggc gacggtgtcg gtcagcgggg
     1741 tgcgctggcc gctcgaccgc gccgccctcg ccgctggcac cacccgcggc gtcagcaacg
     1801 aggccgtcgg cggccccttc gagctgcggg tccacgccgg cgccgtgctc tgcatcaccc
     1861 cccgaaccga ggagaccccg tgatcccgat ccgacgaccc ttgtccgccc tgctcgccgc
     1921 caccctggtc gtggctgcct gcggcgacga cgacgccgag accacgccgg agccggacgg
     1981 cggtggcgcc ggggccacct cggtgaccct gctgacccac gacagcttcg acgtgtccga
     2041 cgaggtgctg gccgccttca ccgaagacac cggcatcacc gtcgagctcc tcccgtccgg
     2101 cgatgccggc tccatgctga accaggcgat cctcaccaag ggctcgcccc agggcgacgt
     2161 cctgttcggc gtggacagca cgttcctgtc ccgcgccctc gacgaggacc tgttcgtggc
     2221 ctacgagtcg ccggcgctcg agggcgtcga cgagtcgctg gtgctcgatc ccgagcaccg
     2281 cgtgacgccc atcgacaccg gcgacgtctg cctcaactac gacaaggcgg gcctcgccgc
     2341 cgccggcttg gaaccgcccg aggacctcgc cgacctcgcc gaccccgcct acgccggcac
     2401 cctcgtcgtc gagaacccgg ccacctcgtc gcccggcctc gcgtacctgc tcgccaccgt
     2461 cgccgagttc ggggaggacg gatggcagga ccactggcgg gccctgcgcg ccaacgacgt
     2521 ggaggtggct gcgggctggg aggaggcgta ctacggcagc ttctccggcg ccgccggcag
     2581 cccgggggac cggcccatcg tggtgagcta cgcctccagc cccccggcgg aggtgatctt
     2641 cgccgaggag ccgctggacg acgcacccac gggggtcgtg gacggttcgt gctatcgcca
     2701 gatcgagttc gccgggatcc tggcgggcac cgagcacgag gcggccgccc agcagctgat
     2761 cgacttcctc ctctcgccca cgttccagga ggacgtgccg ctgaacatgt tcgtgtaccc
     2821 ggtgatcgac gtcgagctgc ccgccgagtt cgtcgagcac ggggccgacc cggaggaccc
     2881 gtacctgctc gacgccgacg tcgtggcggc gaaccgcgac gcctggatcg aggagtggac
     2941 cgagatcgtc ctgcgatgat cagaccgtga gctccgccga gcgagcgccc cgggcgtccc
     3001 gagccgtcac cgccgctgcg ttcgcggtgc cggtggcgtt cctcgccgtg ttcttcgccg
     3061 tgccggtggc gacgttgatc accacggggc tgcgcgaggg cgggcggtgg gagctcggcg
     3121 ccgtgggcga cgtgctgacg tcggccagca cgcggtcggt ggcgtggttc acgctctggc
     3181 aggcggtgct gtccaccgcg gcgaccgtcg tcgccgcgct gccggctgcg tggctgatcg
     3241 ggcgcgtccg gttccctggc cggtcgctgt tcgtggccgc cctcatcgtc ccgttcgtgc
     3301 ttcccaccgt cgtggtgggc acggcgttcc tggccgtgct cggccccgat ggcccggtgg
     3361 cagcgctcgg cggcgtcctc ggcgccgacg tggacctgcg gcgcacggcc accgccgtga
     3421 tcctcgccca cgtgttcttc aactacgcgg tcgtggcccg caccgtgggc gcggcgtgga
     3481 tggcggtcga ccccgccgcc gaggacgccg cccgaaccct gggcgctgac cgctggacca
     3541 cgttccgcac catcacgctg ccccagctgc ggcccgcgct ggccgggtcg gcggccatcg
     3601 tgttcctctt caccttcacc tccttcggcg tggtcaagat cctcggtgga ccgcgcatcg
     3661 ccaccatcga ggtcgagatc caccgcctca ccacccagct gctggacctg cgcaccgcgt
     3721 cggtcctgtc cctgctccag ctcgccgcgg tcatcgccgc cctggcgctg taccggcgag
     3781 ccggcgtccg ccgggggagt gcacgcaccc gcgcgggccg ctccgtcctg cacccaccgg
     3841 ccggcgcgga gcgctgggtg ctggcagcca ccatcagctc cgccgcgctc ctcgtcgggg
     3901 tcccgatcgc caccctggtc tggcggtccg tcgacaccgc cggcgggccg ggcctgggct
     3961 actaccgggc cctggggtcc accggacggg ggagcgcgct gttcgtgccg cctgccgagg
     4021 cggtgcggaa ctcgctgctc atcggtgcag cggcggccgc gaccgccctg gtggtgggcg
     4081 ggctggcagc cgccggcctg caccgcctcg gcgggcgggc tcgcgggatc gcggactcct
     4141 cgctcatgct ccccctcggc gtcagcgccg tcaccgtcgg cttcggcttc ctcatcgcgc
     4201 tcgacgagcc accgctcgac ctgcggacct cgccggtgct gatccccgcc gcccaggccg
     4261 tcatcgccat cccgttcgtg gtccgcacgc tgctgcccgc gctggacgcc gtgccgggtc
     4321 gattgcgaga ggcggccgcc gcgctcggcg caccaccgca ccgggtctgg tgggaggtgg
     4381 acgcccgcct gctgcgccga cccgcgctcg gcgcgctcgg cttcgcctac gccatctccc
     4441 tcggggagtt cggcgccacg gtgttcatcg cccgggccga cgcgccgacc gtgcccgtgg
     4501 ccatcttccg ggcgctctcc cagcccggcg cgctgaacgt cggccaagcc atggccctgt
     4561 ccacgatcct gctggtcctg accgccgccg tgatgctgct cgtggaccgg gtcggcgacg
     4621 cccgggtcgg cggcttctga cgtgtcgtcg ctgatcgtcc gcggcgtctc caaggcgttc
     4681 ggtgacctcg ccgtcctccg cgaggtggat ctcgccgtcg aggacggcac cacgctcgcg
     4741 ctcctcggcc catccggcag cggcaagtcg acgctgctgc gcatcgtcgc cggcctcgag
     4801 cagccggacg cgggctccgt gcacctgggc gacgccgacg tcaccggtcg tcccgcccac
     4861 cggcggggcg tcggcgtcgt gttccaggac caggcgctct tcccgcacct cgacgtggcc
     4921 ggcaacgtcg gcttcgggct gcgcatggcg ggggtcgacc gcagggagcg ggccgagcgg
     4981 gtccgggacg ccctcggcct cgtcggcctg tcgggctacg agcgccgcga cccggccacg
     5041 ctctccggcg gcgaggccca gcgggtcgcg ctggcccggg cgctggcgcc tcgcccggcg
     5101 gtggtcctcc tcgacgaacc cctcgccgcg ctcgaccagc tgctgcggga gcgactgctg
     5161 gtcgagctgc gggcgctgtt cgacgacctc gacgtcacgg tcgtcgccgt gacccacgac
     5221 ccgaccgagg ccgcagcgct cggtgaccgc atcgccgtgc tgctcgacgg ccggatcgcc
     5281 caggtgggga cgccgggtgc ggtcatcgcc gcacccagct cggccgcggt cgccggcctg
     5341 gtcggccagc ggaacgtcct ggccggtcgg gtcgtgcccg gtggcgtgga gaccccgctc
     5401 ggcctgctcc cggtcgatcc cggtcgttcc cgtggggagg tcgacgtcct ggtgccgccg
     5461 tcggcgctgg tgccgaccgc ccaaggcagg gtgcgcagcg tcgtccgtgg gctgctctac
     5521 cgcagcggca cgccggtcgc cgtggtggac gccggcgggg tcgcgctcga cgtgctcgcg
     5581 gagccgggct gggtcgtcgg tgacgaggtg gcgctgcgga tcgtcccgga ctcggtgcac
     5641 ctgatgggcc gctgatcggc gattgaaccg atcgtggtcg cgctgcgtag tcctcaccga
     5701 gatcgacctc aggaggacgc gatgcgacga ctgaccatga tcctcgcggc actgacactg
     5761 gtggtcgctg cctgcggcga cgacgacgac ggatccgccg caccggccga cgacgaggcg
     5821 gtgacggctc cgacgacggc accggccggg ctcggcgcgg aggacgagac cgacgacggc
     5881 acgtcaccga gcggcgaggc agaggtctcg atcagcgact tctcgttcca gcccgaccgc
     5941 atcgatgtcg gggctggcgc gacggtcacc tggaccaacg acgacggcgt cccgcacacg
     6001 gtgacggcag ggaccgcgga cgccgtcggg gacaccttcg acgagccggt cgaggccggc
     6061 cagaccgccg aggtcacctt cgacgaggcc ggcacctacg actacttctg cgcgatccat
     6121 ccgaccatga ccggtcaggt cgtggtctcg tagagcacgt ggcccatgcg gacctggacg
     6181 cggcggtgcg gcacctccac gccgcccacg cgcccgcgct ccacgcgtgg gcccggcacc
     6241 gcaccgatga cgcccgggag gccgaggaga tcgtgcagga gaccctcatc ctcgcctggc
     6301 gcaagcagga ccagtacgac ccggcccgcg gctcggagcg agcctggctg ttcggcatcg
     6361 cccgccacgt cgccgtcgac cgccatcgtc gacagcagcg ccacctccag gtcgtccccg
     6421 gtgccgacgc ccccgaaccg gtggacgacg gtgagatcga ccgtgcggtc gagggctcgc
     6481 acatccgcga tgccctgtgg gccctgtcgg acgcccatcg ccaggtcgtg atcgagacct
     6541 actaccgggg tcggagtgtg cgcgaggctg ccgctgcgct cgagatcccc gagggcacgg
     6601 tgaagtcccg cctctaccac gcgctgcggg cgctccgtcg cgagctcgag cgtcgagagg
     6661 tgctgccatg accgcctcgg accacggccg ctaccgcgac ctgaccgcgg cctacgtgct
     6721 cggtgccctc gacgccgggg agcgggtcga gatcgagcag cacctcgagg cgtgcgaggc
     6781 gtgccggagc gacgtcgtct cgttcgcgcc cctgcccgcc ctgctgagcc gcgtcgatgc
     6841 cgacgatgcg gccagcgacc acccgggagc cggggccgac gccctggtcg ccgcggtccg
     6901 agccgacgtg gaccagctcg accgcagccg gcgtcgctgg cggcggggcg ccggcctggc
     6961 tgcggctgcc gcgctcgtgc tggctgcctt cgtggtcgtc cgcgacgacg acccggcccg
     7021 gtggtcggac ggggtcgagc tggcggtgga ggcgatctcg gccgagacca ccgccgacgt
     7081 ggtcgcgacc gagcgcgggt ggggcaccta cctctccctg tcggccgagg gcctcccgcc
     7141 acgcgacgcc tacgagatgt ggacggtgga cgacgagggc gcctgggagc cggtcgggtc
     7201 gtggggcccg acgccggagg gccgggccga cctggggtgc tcctcggccc tgccgctcgg
     7261 tgccatcgac cgcgtcgtgg tcacctccga cgaccgcggc gacgagctct tggtcgcccg
     7321 gtgaggggcg gtcagcgcag gtggacggtg ccgtcgtcgt ccaggcgcca gatcgggttg
     7381 tgggcgacct cccaggggtg accgtcggga tcgatgaaga tccccgtgta gccgccccag
     7441 aacgccttcc cgccgggtcg ggcgatggtc ccgccggcgg cttcggcctg cgcgagcact
     7501 gcgtcgacct cttcggggga gccgacgttg tgcgccaggg tcacgccgcc ccagcccccg
     7561 gggtcggtga cctcgctgtc gtgggccagc ttctcgcggc tccacagggc caagaccatc
     7621 cctcccgcct ggaagaacac cacctcaccg tcgggggact cgccggacca gccgagggcc
     7681 tcgtagaagc gccgggcccg atcggcgtcc tcgacgccga gggtgatgac cgagatgcgc
     7741 ggctccatcg cgcggacgct aggcgaggtc gcggtcagcc ggtcgggcaa cctcgtggcg
     7801 gagcagtgcc ggtgagcagc cggaagcctg ctgccagctc gtcacacgac gatgcaccga
     7861 gccggtacgg cacgaccggc tcgtccatca ggccccaggc caggacctcg gcaccgtgct
     7921 cgacaccccg tcgatcccac gacgtggcgg gatcgtccca cgccgtcaga cctcggaggg
     7981 cggcgaactg ctcctgcgcg tcgagatcca cgtgatcgct catccacgcg tgggcgagct
     8041 cgtggaggag gagctgtcgc cagcgcaccg agacggcctg gcacccgggg tccgtgcaga
     8101 tgtcgtcctc ggtcgcgcag atgtagatgg ccgaaccgcg ctcgtcctcg atgcagcacc
     8161 cgtcgcgctc gttcgggcac tcgtcggggt ggaggtcctc gtcgaagccg acgagcgcga
     8221 cgtgggggac cgtgaggcca ccggcgacga agcgttcgag gccccatcgg acgtagcgct
     8281 cgagcgctgg cgtgccgtcg tgcacggtga cctgcacacc ggccacgacg accgtcccgg
     8341 tcacctcgac ggggagcgag ggcacgacca gccccgtcca ccagccctcg ggaggggtga
     8401 ggtcgtcggc gcagcgccgg gtcgcgccga tctcgtggta gcggcgctcg tgcccgacca
     8461 ggccgtcgcg cacggtgagc gcgaccacgg tcgtccctgg gcagcgcgcc ggccccccca
     8521 gcgtctggat gaagaagagc tggtacggga cgctcggccc ggcgggcagc gcgtccggga
     8581 ggtacacgta catggccccg cgaccgtcca cgtcgagcag gaccgccggg ggcgtggccg
     8641 acggtgcggc gaaccgcttg gcgagggcgt ggctggcgat ggcgtcccgc ccggtcagct
     8701 ggagcccgac gagcccgtcg accagctcgg cgtcaggggc gtacctcgct gcgacggcgg
     8761 ccgggtctgc ggcggaccag acctcgaggt gggctgcggc cagctcctcg agggatgccg
     8821 tcgccgcggg acccagcacg gctcggacgt cgtcgaccga gcggggctgc agctccctgg
     8881 tgacaagacc ctccgggccg atgtcctgca cgacgagctc ggaggcgacg ccggaggtcc
     8941 aggagcggtt gaccatgcgc accgcacctc tcgggtcgag gaagagcatc cccgtggtcg
     9001 tctcgtccgt cgtcgccccg aaccgatcgg tcagccactc ctcgcaggct gggccgccct
     9061 cgcatgccga gccgctcgtg taccaggtgt ccacgagggc gtcggcgtcg tagaagcgcc
     9121 ggacctgctc ggtgatggac cgagcaccgg tttcctccat ggcctcgaag tacaccaccg
     9181 cctcggcggc ccagggcagg tcggtggcag gtgcgccaca ggacgagacg gacaccagcc
     9241 cgaacgcacc gaccgcaagt agccgtcgca acacgtcgac ctcctccgtc gctgaaacgg
     9301 tacggaggca cagcgagggt ggccagagtc gcccgtcccg ttctgagcgg agatgacgac
     9361 cccggcccgg tggctcagac ctgcgagcgg ccgcgctcgg tgacggcctt gcgtcgcgcc
     9421 tcgaggtcgg cgccgtcgcg cccgccggcc tgccactcgc cggccacccg gccttcgagc
     9481 gcccaggcct cgccgcgcgt gatcagcgcc ccctgctcgt aggtctgcag catctggcgg
     9541 acgccggccc ggtcgttggt cgcgatgtcc gcggcgagct gctgggcgaa gggcacgagc
     9601 tcgtcgtggg gcaccacgtg gttgaccagg ccccaggcca gtgccgtgct cgcatcgagg
     9661 aagttgccgg tggtcgacag ctccttggcc cgccggaagc cgacggcctc gggcagcagg
     9721 acggtgaggc cccagccggg catgatgccc atgcgggcat gggtgtcggc gaaggcagcc
     9781 cggtcggagg cgacgaggaa gtcgcacgcc agggcgacct cgaagccgcc ggtgatggcc
     9841 acgccgttga ccgcgccgat gacgggggtc tccatcggcg ggatggggcc gcggccggtg
     9901 gcggtgtccc cggtggcgcc cgtctcgccc agtgcagcgc cggaagtcga tgccagctcc
     9961 ttcaggtcca gtccggcgca gaacgcaggg tcagcgccgg tgaggacgat cgccgcgacc
    10021 gagacgtcgg cttccagctc cgacatggcc tgccagagcg acttgcgcag ctcccggctc
    10081 agcgcgttgc gggcgccggg ccggttgagg gtgacggtgg cgacggcgtc gttgcgctcg
    10141 acgaggacga cgggatcgga catggcgggc tccgtttggg ttgggtgagc gtgggtcgat
    10201 cagtaggggt cgtggtaggc gagcgggagg tcgccgaggc ggacgagcat cgaggcgatc
    10261 cacccaccga gggcggagaa gtggtcatcg aggatggcac cgtcgccgag cgccttgtcg
    10321 tccagctcgt aggccccctc gtaggccagc tcgagcgcgt ggctgaccgg ctccagctcg
    10381 gcggtgtagg cggtctccac cgtgggcgcg gtgcggtgca gctggtccgg tccgacggac
    10441 gggctctcgg cggggagcac cgcggtcacc tggtcggggc tcggcagctc gacgaggcgc
    10501 tggagccgga gcacgatgct gatctcgatg cgagggtcgt cctcgggctc ctcgttgcgc
    10561 agccacgacc ggaacgccga ctgcgaccac gtcggccagt cgagcgtgat gtcggctcgc
    10621 acgcgcggcg ggttgccctc gccggggagg ccgtaggagg tctcccacgt cgtgtcgccg
    10681 agcagcacgt cgacctggaa ccgctcctcc gccgtcatcc gctcgagcat cgccgtctcg
    10741 agggcgtctc gcagggcacc gatcgcgtcg gtgaagacgt ggtccaacat gggttggcga
    10801 ctctaggggg tcgtccgctg cgcttctggg ccgctccaca cgcatccgat gcgtgcacag
    10861 cggcccggtt cgaggatgtg accggcttct gcccctcagc tcgaacgctc gagccgcgag
    10921 gcggcctgct cccagtcggt catcaggcgc acggcctcgt ccttggctgc gtcgagcgcg
    10981 gtggtgagct cggtgagctt gtcggcgtcg gcgtagacgt ccgggttccc gagcgccgcc
    11041 tggagctcgg cgaccttggc ctcggccgtc tcccactgct tctcgagccg gttcacctgc
    11101 ttgcgcaggt cccggtcctg ctggctgccg ccaccacctt gcttctgctt ctgcttcggc
    11161 gaggtggggc tgctctgccc gggtgccttc gccggctggg tcgggccctt ggccttcgcc
    11221 gccttccccg aacggccacc gccgctcttc ttcgcgctct tcaccggcga ccccgacgcg
    11281 tcccggcggg tggtcgcggt cgtcggtgtg agcacggcct cgtccacgcc gtggtggaac
    11341 ttcgccgtgc cgttgcggac ctcgatgagg tcgtcggcaa cggagcggat caggtagcgg
    11401 tcgtgggtca cgagcaggac ggtgcccggg tacaccttga gggcgtcctc gagcatgtcg
    11461 cagctggcca ggtcgaggtg gttggtgggc tcgtcgagga tcagcaggcc ggcgggttcg
    11521 gccatcgcct tggccagcgc gaggcgcgtg cgctcgccac ccgagagcat gccgacctgg
    11581 cgctcgacgg cgtcgtcgcg gaagccgaag ctggccaggt aggtgcgcag cgtcttgccc
    11641 cggggcgtgt cgctgccgat gccgtcccga gccacctgca ggacggtctg gtcgggcgcc
    11701 agctcgtcgg cctggtgctg ctcgaagcgg gcgacggtga cggactggcc gatctcggcg
    11761 gtgcccgtca tcggcgggag gtcgcccagc atcatcttca gcagcgtggt cttgccggcg
    11821 ccgttcggcc cgaccagggc gatcttctgg ccgcgttcca cgtgcaccga cacgtcgcgc
    11881 aggatcggcg tgtcgccgta gccggcggtc atgtgcgaca gctcggcgac cacccgtgac
    11941 gtgcggggcg gctcggggaa ctggaagcgg gcctgcagct ccttgcgatc ggggatctcg
    12001 atcgtctcga gcttctccag catcttgatg cggctctgca cctggcgggc cttggtggcc
    12061 ttgtagcgga accgctccac gaagcgctcg gtctgggccc gggccttggc ctgactggcc
    12121 gcctgggcct cgagctgggc gatgcgctcc tcgcgctgca cgacgaactc ggcgaagccg
    12181 ccgacgtact cggtgagggt ctcgttggcc agctcgacca cgtgggtcgc gacgttgtcg
    12241 atgaagtcgc ggtcgtggga gacgaacagc agggccccgg tccacgcccg cagccggccc
    12301 tcgagccagc cgacggagtc cacgtcgagg tggttggtgg gctcgtcgag caccagcagg
    12361 tcgggagcgg acagcagcag ccgggccagg gcgacgcgca tgcgccagcc gccggagagc
    12421 tccgacacct gccggtcggc gtcgtcgggg gcgaacccca ggccggcgag caccgtgtgg
    12481 gcctcggctt cgagggcgta gccgttcagc tgctcgtact gcgtctgggc ctcgccgaag
    12541 cggtgcagcg cctcgggcga ggcatccgcc gggccctcca cgatctggtg gtgcaggcgc
    12601 atgatgtcgg gccggccccc gagcacggtg tcgaggacgg tgccgtcgtc ctcggacggg
    12661 atctcctggg ccaggtagcc gatccgcatg tccttgggct tggtgaccgt gccgctgtcg
    12721 ggctcggcgt cgcccaccag gatgctgagc agcgaggtct tcccctgccc gttgccgccg
    12781 accatcgcca cccgccgccc ggccgagagc gacagcgtga cgtcacggaa cagcacgcgg
    12841 ctgccgtgcg cgagggagag tccggaggcg gtgagcatgg ccgttcgagc ctacggaggc
    12901 gctccgcgaa gccgcgaaac agatcgtccc cgtcgtcacg cccgaccggt accgtcgctc
    12961 cgatggctga cggacccctc gaccccgacg cgctcgtgcg ggcccggggc ctgacgaagc
    13021 gcttcgacga cttcgtcgcc gtggacggca tcgacttcga ggtccgccgg ggcgaggtct
    13081 tcggcttcct cggcccgaac ggcgcgggca agacgtcgac catgcgcatg atcagctgcg
    13141 tgtcaccggc cacgggcggc gagctgtccg tgctgcacct cgatccgagc agcgacggcc
    13201 ccgccatccg cagccgcatc ggcgtcgtcc cccaggagga caacctcgac accgagctgt
    13261 cggtctggga caacttcatc gtctacggcc ggtacttcgg cctgccccgc cgggtgctgc
    13321 gggagcgggc ccaggagctg ctcgagttcg cccgcctcac cgatcgccgc gacgaccggg
    13381 tcgatccgct ctcgggtggg atgaagcggc ggctcaccat cgcccgctcg ctcatcaacg
    13441 atcccgatct cgtcctgctc gacgagccca ccaccggcct cgacccccag gcccgccatc
    13501 tgctgtggga ccgcctctac gggctcaagc ggcgcggcgt caccctcatc atcaccaccc
    13561 actacatgga cgaggccgaa cagctctgcg accgcctcgt cgtcatggat cagggccgca
    13621 tcgtcgacga gggctccccc cgccagctca tcgaccgcca cgtcacgccc gaggtcatcg
    13681 agctgcgctt cgccgagccg gccgagcagg aggccgcgct gtcgtctgcc ggcgaggctg
    13741 ccgagcgcat cgaggtgctc gccgatcgcc tgctcctcta cacccgtgac gccgaggcca
    13801 cgcaccgggc actggccgag cgcagcgtcg aaccgctgac cgagctgacc cgccgggcgt
    13861 ccctcgagga cgtcttcctc gccctcaccg gccggacgct ggaggagtga gccggtgcgg
    13921 atgacccagt cggtgtggcg ggccctcgag cgcgagctgc tcgtgttcgt gcgcacctgg
    13981 cgggggagcg cgttcaccgc catcgtgcag ccggtgctgt tcctgctggc gatgggcatc
    14041 ggcctcggcg gcctggtcga cgagggcgcc ggctcggcca gcgtgacgct cgactacctc
    14101 gacttcgtcg cccccggcct gctcgccgcc tccgcgctgc tcgccgtggc gggcgaggcg
    14161 ctctggggcg tcatgggcgg cgtgaagtgg atgggccagt tcaaggggat ggtggccacg
    14221 cccatgacgc ccggcgacgt gtacggaggc cttgtcctgt ccgccggcgt gcgcgccggc
    14281 gccgcggcga tcgccttcct cgtggtggcc gcggtcctcg gcggggtgtc ctcgctgtgg
    14341 gcgccgctgg ccatcctcgc cgcggtgctg ctcgccatcg tcacgatgag cgcgctgtcc
    14401 gcctacgccg tgcgcaagga ggacgaccgg accttcccgc tcatcatgcg tctcggcgtc
    14461 atcccgatgt tcctcttctc gggcaccttc ttcccggtcg agcagctgcc cgacctggtg
    14521 caaccgatcg ccgtcttctc accgctctgg cacggcgtgg aggtcgcccg cgacgccatg
    14581 acgggggccg tcgacggctg gacgctgctg cacgccggcg tgctggtcgc cctcaccgcc
    14641 gtggtcctcc cgatcggcgt ccgcggcttc gagcggcggt tgacgccgtg agcgtccagg
    14701 acctggtcga ccacggccgc atcgcaccgc cgctcgaccc gcgcaagcgc ttcctgcgcc
    14761 tgaccgagcg caacgtcgtg ggccacctca aggcgtggcc gatcctgctc accggcgtgc
    14821 tcgaaccgtt cctctacctg ctctcgatcg gcatcggcgt cggggccctg gtcggcgaca
    14881 tcacctacgc cggccgccag gtcccctacg agaccttcgt ggccagcggg atgctggcca
    14941 cggccgccat gaacggcgcg gtgttcgaca ccacgttcaa cttcttcatc cgcctccgct
    15001 acgccaagct ctacgacgcg gtgctggcga cgccactcga accccgcgac gtcgcactcg
    15061 gcgaggtcac ctgggcgctg atgcggggca ccttctactc ggcggcgttc ctcgtcacga
    15121 tggcgctctt cgggctcacc gagtcgtggt gggcgatcgc cgccctgccg gccaccgtgc
    15181 tgatcggcgc ggcgttcgcc tcgatcgggc tcgccggcgc cacctacctg cgctcgttcc
    15241 tcgacttcga cttcgtgaac ctggccatca ccccgatgtt cctgttctcg gcgacgttct
    15301 tcccgctgtc gcagtacccc gacgggctgg ccgccgtcat ccggttcacg ccgctgtacc
    15361 agggggtcgc cctgtgccgg gcgctcacca ccggcgaggt cggcatcggc ctcctcgtgc
    15421 acgtcgccta cctcctcggc atggtcctgg tgggcgtcgc ggtcgccagc cggcgcatcg
    15481 acggcctgct ccgctcctga cggttccgac ttccgtccgg cacttccggg tacggagcac
    15541 gggagcccag acccctgccc gtctccccga ggagccccgc catgatccac gagatcgaca
    15601 tcgacctccc cgtccgcacc gtctacgacc agtggacgca gttcgaggac ttccccctgt
    15661 tcatgaagca cgtgaccgac gtgcgccagg tggatgacac ccacacccac tggaaggcca
    15721 agatctccgg cgtcagccgc gagtgggagg ccgagatcac cgagcagacc ccggaccagc
    15781 gggtcgcctg gacggccacc gacggcaccg ccaactccgg cgtcgtgacg ttccacgccc
    15841 tcgacgacaa caccacgcgc gtcgtgctcc agatggagac cgacccgaag ggcttcctcg
    15901 agaaggtcgc cgactggggt ggctacatct cggaccgcgc caagaaggac ctcgaccagt
    15961 tcaaggactt catcgaggcc cgcggcaagt ccacgggtgc ctggcgcggc caggtcgagc
    16021 gcgactccat gcgcgacctg cactcccgcg aggacgagct ccgcgagctc agcgaccacg
    16081 agctggccaa gcgggccgag gatgccgggc tcgagcgtcc gctcgaccgc aaccgcgact
    16141 ggctggtcag cgccctcgcc cgtgatggct acgaccgcaa caccagccgc gaccacgacg
    16201 gcgacgacga ccgtcgggac tcggaagacg cgcacacgtc ctgacgtgcg agcgagcggg
    16261 tgacggtcag gccgtcgccc gctcgtgcgg ggtcatcagc gcgatggtcg gaccgatgcc
    16321 gagctcgcgc gtgcgcacgg tggcgatccg ccgccccggt tgcactgcgg gcttcgacgg
    16381 cgagcaggcc tcgccgagct gcgcgaccga tgcgtcgatg tccggggtga cgagcgcgag
    16441 gccccagatg ctgcagggcc cgtcgccggt cgctgacggc ggaccgacca gctcgaggat
    16501 cacctcaccc atccggaaga agcgctgctg catcgcccga tcgccgctgc cgacgtcccg
    16561 ggtgcggcgc aggtccagac ccagcgcgcc gaaggcctcg gtggtgcggt cgagatccgg
    16621 cgagcggagc acgatgtgat cgatccgcac caccccgttg gggtgctcga ccggtgcggg
    16681 tggcggccga tcggtgacgg acgtcgcgat cccgtccacc gagcgcggca cgtcctcgtc
    16741 gtcggcgcgg agtcgccaac ggtcggctcc ggcaccgtcg ccggtgcaga cgacgcgcac
    16801 ggtgccgatg gcgacctcgc tgccgtcgac ggcgaagccg gccgacgccc acgccgccgg
    16861 ttcgtctccc acctcgagcg cgtccaacca caccgtcacg cgcgccggtc taccccatcg
    16921 cccgcgaccc ttcgcctcgg atcggaagga agtcggcgat cgccctcaac tccgggccgt
    16981 cccggtcgaa ggggggaggt gaccggtcga gtccgcagcg cgagggagca cgaacgagca
    17041 tggcgatcga gcgatcatga cgatgtccga gacgccggtc tcgccggcga cggtgtcggc
    17101 gctgatgcgc ctgaccgcag acccggaccc ggcggagaca gatctggccg gcgtcgtggc
    17161 cgccgacacc gggctggcgg cgcgcgtgct ggcggtggcc aactccgcct ccttcgggct
    17221 gtcccggcag gtcacgcagg tccaccaggc cgtcggcctc gtggggatga acctcgtgca
    17281 gacgctcgcg atcgccgggt cctgccagct cctcgacggc acggtggggc tgccccgtgc
    17341 tcgccgccat gccgtggagg tggcctgcgc cgcccgagcg ctggccgagc gtgccggcct
    17401 cgacggcggc gcagccttcg ccgcggggct gctgcacgac ctcggtgaga tcctgctgtg
    17461 gcgggacgac cccgagccct acgccgaggc ccacgctcgg tggagcgacc cgacgacgca
    17521 gctccgcgag gagcgcggcg cctacggcac cgaccacgcg ctggtcgccc gggagcagct
    17581 ggcggcgtgg aacgtgccgg ggaacatcgt cgatgccgtc ggggaccacc accgcccgga
    17641 cctcctgcac cgggacatct gcacggtcgt ggtcgcggcc gaggagctca ccgaccgcga
    17701 cgtcgactgg tcgccgcggt tcgagatctt cggcgtcacc ggtgccgcgc tcccgtcgct
    17761 gcgggacgag gtgtcgacgc agaccgacga gctgctcagc ctgctcgtgc ggtgatcagc
    17821 ggttgacggc ggagagcacc gctcggaggg aggcgaccac gatgctgtcg tggacaccga
    17881 cgccccagcg caccaggccc tccggggtct gggtctccac gtaggccacg gcctgggtgt
    17941 cggcaccggc gccgatggcg tgctcggtgt agtcgaggac gtcgacgtcg acgccgaggt
    18001 gctcgcgcag gccgttcacg aaggccgaca ccggcccgtt ccccttcccg atcaccgtgt
    18061 ggtgctcgcc gtcggcgatg acctgggccg tgatctcggc ggcctcgtcg gaggtgcgca
    18121 tctcgtagct gtcgagccgg tagcggccga tgccgaggta ctcgccctcg aactgggccc
    18181 acatctcccc ggggttgatc tcggtgccgg tgtcctccac cacgccctgg atcgccttgg
    18241 agaactcgat ctggagccgg cgggggaggt cgaagccgtg ctcctccttc atcacgtacg
    18301 cgacgccgcc cttgccggac tggctgttca cccggatgac ggcctcgtag gtgcggccca
    18361 cgtgcttggg gtcgatgggc aggtacggca cctcccagac cgggtagtcg cccgccgcgc
    18421 cgccctcggg gatcgtctcg gccagggcgg ccaggccctt cttgatggcg tcctggtggc
    18481 tgccggagaa ggcggtgtag acgaggtcgc ccccgtaggg gtgccgcggg tggacctcga
    18541 ggcggttgca gtactcggtg gtgcggcgga ccgcgtcgat gtcgcggaag tcgatctcgg
    18601 ggtcgacgcc ctgcacgaac aggttcatgc cgagcgtgac caggtcgacg ttgccggtgc
    18661 gctcgccgtt gccgaacagg gtgccctcga cccggtcggc gccggccatg acgccgaact
    18721 cggcggcagc cacggcgcag ccccggtcgt tgtgggggtg caggctcagg acgacctggt
    18781 cccggtgggg gatgttgcgg tggaagtact cgatgacgtc gccgtagatg ttcggcgtgt
    18841 acatctccac cgtcgccggc aggttcatga tcagcttgtc gtcggtgccg ggatcgatgg
    18901 cctcccgcac cgcgtcgcag atctcgatgg cgtagtcggt ctcggtgccg gtgaagctct
    18961 cgggcgagta ctcgtagcgg acgtgggtgt cgggcacccg gtcctcgagc ttgcgggcca
    19021 gcttggcggc gttgaccgcg atgtcggtga tgccgtcctt ctccaggccg aacacgaccc
    19081 ggcgctggag gacggaggtc gagttgtaga agtggacgat ggcgcggggt gcgccggcga
    19141 tggcctcgta ggtgcgctcg atcagctcgg gccgggactg caccaacacc tggatccaca
    19201 cgtcgtcggg gatcaggtcc tcttcgatga ggtgccggca gaagtcgaag tcgggctggc
    19261 tcgccgacgg gaacccgatc tcgatctcct tgaaccccat gccgaccagc aggtcgaaca
    19321 tcttgcgctt gcgcaccggg tccatcgggt cgatcagggc ctggttgccg tcgcgcaggt
    19381 cgaccgagca ccacagcggc gcggcctcga tgcgctggtc cggccaggtg cggtcgggga
    19441 gctcgagcgg gaggaacggc tggtacttct cgaatggcat cgtgccgggc ttgccgttgc
    19501 ggggttcggt tcccatgtcg gttcctctcg gggacttcgt cgggctgctc tggttcgggt
    19561 cggaaaagca agaaacctcc tggccgggag gcacaggagg tcgggcgagc gcggtatgtg
    19621 gcgctcgcct aggtgagaag gagcagtccg agatcgtggc tcatcgctcc ccaggatgcc
    19681 ggggtacccc cgcgatgtca acgcagatgg ctcagtagta cgtgcagcgg caccggtggc
    19741 ggggccgggc cttcaccgcc gggtcctggc cctgctgcca gagccggtgg tcgagcgtcg
    19801 ccgcggtgac ctcgtcgtgg ccggcggacc gcaggcgatc gaccagcagc tccacggcgt
    19861 ggacgccgag ggcccgcagc tcgacctcgc cgcgctcacc cgcctcgagc agctcctcgc
    19921 gctcgatgcg ggcatcgagc gccgggtcca ccaccagcac cccggccatc ttcaggacgt
    19981 ggggcaccag gttgtcggcg aaggcggtca gggtggccag gtcgtcgaag cggccgagct
    20041 cgtcgtcggc cagcgcgccc gccaggttcg acacgacgat ctgggcccgc ttgtggagcg
    20101 gcacctcgag gtcgtccagg cggtggcggt cgtcccagca ggggagcgca gccagctccg
    20161 ccgccagcga tgcaccgctg ttgccggcgg cgccgaccat cgccgccgcg gagccgccgt
    20221 ggtgctcgag caggtggacg cccagctccc gccacgcagc ggtgaaggcg tcgagcaggc
    20281 cgcgcacctc accctcgagc ggctggtgga acacgccggc cacctcgtcg accgtgacgt
    20341 ccgcgagcca ctcggcgcct ggcaccccgg cggtccggca gtgctgctgc cagcgggcgc
    20401 ccatcgtccg ggcccccgag tgcccgggca gcttcacgac gtgggggaac cagcccgacc
    20461 cgaagttgac ggcgttccag gccaccacgc ccatcgcccg gtcggcgacg ctgccctccc
    20521 agcgctcctc ggggaagggc tcgggcgcgg tcgggtcgag cgatgcagcc agacggtcga
    20581 gcgcctcgtc gtcgatgcgc accagctcgg cgcgaccggc cagcgcggcg gcaccggtgc
    20641 ggacccggtc gggcagcgga gcgtcgtcgt cggccatggg agactcgcag cgtgccagag
    20701 ctgccccggt tccacctcgc cgtcccggtc gacgacctcg ccgccgcgga ggcgttctac
    20761 ggcggggtgc tcggatgccc caccggacgc cgatccgacc agtggatcga ctgggacctc
    20821 cacggccacc agctggtgac ccacctggtg gagacccgtc ccggtgccga cgacaccaac
    20881 ccggtcgacg gtcaggacgt gccggtgccg cacttcggcc tgctgctcga ggtcgacgcg
    20941 ttccacgcca tggccgagcg gctccgggcc ggtgaggtgc cgttcgtcat cgagccgtac
    21001 ctgcgcttcg aggggctgcc gggggagcag tggacgctgt tcgtccgcga cccggcgggc
    21061 aacgcgatcg agctgaaggc cttcgccgac gacgcagacg tcttccgcag ctgagctccg
    21121 tcaccggccg cttccacgcg gacggtggga ggaatcctgg ccccggaccc cgaaatcgtc
    21181 ctcgggtggg cagcccagcc agggaaggga acccatgctc cggatcctcc gttcacgccc
    21241 gcgctcgtcg ctggcggtcg cagccatcgc gctgctcgcg tccgcggtcg ccatctcgcc
    21301 gatcgccgac gcccaccccg atcccctcga gggaggtcac aagcgccagg ccgccggcgg
    21361 catcgtcgtg caggtgaact cgcccgacgg cctcgccggt gacctgcccg ccgtgcagtg
    21421 gggggacacc ggcgagatcg acgggcagac cgcggagctg gcgtgggccg gcagcggctg
    21481 cagcccgttg agctacggca ccgccgacgt cgccgggaag atcgcgctca tcgagtccgc
    21541 cgcggccgac gacccgtgcc cggcctccac cttcttccag cgggtccagt ccgccgagca
    21601 ggccggggcg atcggcctcg tggccatccc ggccgagggc gccgagccca ccgggagcgc
    21661 caccgccgtg accgcgacca tcccggccct cgaggtgttc cgctcgcccg aggtcctcgc
    21721 cgtccgcgac gccgtgctcg ccggcgaggc cgtcgaggtc acgctcgccg acacccgcga
    21781 gccgctgccc cggatcatcg acgagccctg cgtcgacggc atggccggcc cgttcccctg
    21841 tgacggcgtc gacctgctct ccttcgtccc gcaggaggag ttcgacggcg ccggcgtgag
    21901 cgacatctgg ggctggaccg accaggagac cggcggcgag tacgtcatgt tcggcaagac
    21961 caacggcgtc gccctctaca acgtgaccga cccgtacgcg ccgatctacc tcggcgagat
    22021 cccgaacgag gcggcgctgc aggagatctg gcacgacatc aaggtcttcc aggaccacgc
    22081 cttcatcgtc agcgagtcca tgccgcatgg catgacggtc ttcgacctca cccagctgcg
    22141 cggcgtcacc gagccgcagg tgtggaagcc cacggcccgc tacgacggcg tgatgtcggc
    22201 ccacaacgtc gccatcaacg aggagaccgg cttcgcctac atcgtgggtg gcaacgctgc
    22261 gctggtcctg cccgaccagt gcctgtcggg cctccacatg gtcgacatca acgacccgac
    22321 caaccccacc ttcgccggct gctacccgct cgacggcggc cccggcaccg cagcccgcac
    22381 cgccgggctg ccggtgggcg cggcctacgt ccacgacacc cagtgcgtcg tctaccgcgg
    22441 ccccgaccag gaccacttcg agcgcgagct ctgcttcaac gccgccgagg acaagatcac
    22501 catcgtcgac gtcaccgaca agctcctgcc gtcgactgtc gcgaccatcg actacgaggc
    22561 cgtggcatat gcccaccagg gctggctgac cgaggaccac cggtacctgc tcaccaacga
    22621 cgagctggac gagcaggccg accccggcac gcgcacccgg acgatcgtcc tcgacgtcac
    22681 cgacctcgac gcgccggtcg tgcacgacat ccacctgcac gacacgatct cgatcgacca
    22741 caacaactac gtgcacgagg gcctcgccta ccagtccaac tacacgacgg gtctgcggat
    22801 cttcgacgtg gcgacgctgg acgacgtcga cccccggctc gacccggtgg gcttcttcga
    22861 cacctatccc ttcagcgacg acccgacctt cgacggcacg tggtcgaact acccctactt
    22921 cgggtccggc acgatcgcgg tgagcggcat cggcgagggc ctgttcctcg tgaagctggc
    22981 ggacgccggc accgacaccg acaccggtga tggcgatggc ggcgaggagc cggtcccggg
    23041 cagcaacggc aacgggcgcc cggaccacgc cggaccgaag gacggccgcg gcccgaaggg
    23101 ctgagtcccc ggggggaccg gcgtcagctc gtcgggtggg cggtggccca ctcgacgagc
    23161 tggtcggccg cccacgtgtt caccacctgc tcgggcgcga cgccgtggcg ggccgccttg
    23221 tcgcagccga acggctgcca ctccagctgg ccgggggcgt gggcgtcggt gtcgatggcc
    23281 agcttgcagc cccactcgag ggccagctcc agcaggtggt cgggcgggtc ctgccgctcg
    23341 gggcggcagt tgatctccac cgccgtgtcg aaccgggcgc aggcggcgaa caccacgtcg
    23401 gcgtcgaagc tggacccgat gcggccggcg aagtcgctct tgccgatctg gcgctggtgg
    23461 ccgggcacct tctgcccggt gcagtggccg aggatgtcga cgtgggggct ggccacggcc
    23521 atgaccatcc ggcgggtcat ctccttcgcg tccatcgaca gcttcgagtg cacgctggcc
    23581 acgacgacgt cgaggcggtc gagcaggtcc tcgtcctggt cgagggtgcc gtcggcgagg
    23641 atgtcgacct cgatgccggt gaggatccgg aagtcgtccc agcgcccgtt gagctcgtcg
    23701 atcacgtcga gc
//
LOCUS       DATISU010000391         5060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_172970, whole genome shotgun sequence.
ACCESSION   DATISU010000391 DATISU010000000
VERSION     DATISU010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172970"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..565
                     /locus_tag="VLR27_15535"
     CDS             146..565
                     /locus_tag="VLR27_15535"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSP04922.1"
                     /translation="MADNASRIEELYGAFGRGDLDAVLGAMDPQIEWNEAEHVTFWTG
                     APFVGPDAVVQGVFARIPETFGDTFRIEPERFVDCGSTVLVQARYHGVAQATGKEIDV
                     QVAHVWDLGDDGKVTRFQQYTDTWAFAEATGETPEAS"
     gene            complement(573..956)
                     /locus_tag="VLR27_15540"
     CDS             complement(573..956)
                     /locus_tag="VLR27_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04923.1"
                     /translation="MSKILFVDAGLAAGWRRAEPDLDQLDEVVAAARELVDDTVVLAD
                     ASLKWSLPESQQERFEEYRGTAAVLCAPGGTKGGHHAFLATAARAAAARDKDVWVLSA
                     LDLGEGPWRTALLRRPDGYWSIELQ"
     gene            complement(953..1870)
                     /locus_tag="VLR27_15545"
     CDS             complement(953..1870)
                     /locus_tag="VLR27_15545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HSP04924.1"
                     /translation="MSPTETPDASTPIEDRIGPRGIWWPTDAMPVEAAVEFAQWLESL
                     GYGALWIPETLGRDPFAHAAHLLSNTSSLVVATGIANIFNRHPGSMRQAQLTLAEQSG
                     GRFLLGAGVSHAPIVAGVRKLDYSKPLTSMREHLQGMADSMYMAPAPATPPKTVIAAL
                     GPKMLELAATAADGAHPYWTTPEHTAQAKAVLGDDKMLCVEQKVVLCTDAAEARATAR
                     AALGVYIGLPNYFKNWFRLGFDESDLADGGSDRLIDALVPWGTADEIEARVAEHVDAG
                     ATHVCLQPLKPSARMGEPDRDALEALAPR"
     gene            1977..2564
                     /locus_tag="VLR27_15550"
     CDS             1977..2564
                     /locus_tag="VLR27_15550"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HSP04925.1"
                     /translation="MFRALLLAPPGAGKGTQGQRLADRHGVPHLATGDLLRDHVSRGT
                     DLGQQAKSHMDAGRLVPDELVIDMVLRKIGGDGEGEILEDFLLDGFPRTLAQARFAYE
                     WGLQFRRTFHAVIMLEVPEDELVRRLVQRGQEQGRSDDTEDVIRNRLTVYEQNTAPLV
                     DFYESRGILVRVDGTGTVDEVTARIEVGLEPMVPA"
     gene            2561..2944
                     /locus_tag="VLR27_15555"
     CDS             2561..2944
                     /locus_tag="VLR27_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04926.1"
                     /translation="MSRENLAGAQRALAAVIAVGILVMAAIAGRSIFGPWDIVVHGYV
                     GNGLFVLAVVNLALAIYRRPGTAELTVAALIGMLTFAQVGLGYVGREQLEAAAWHIPN
                     GVLLMALSTYQWASLQARAAVSSAT"
     gene            complement(2930..>5060)
                     /locus_tag="VLR27_15560"
     CDS             complement(2930..>5060)
                     /locus_tag="VLR27_15560"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSP04927.1"
                     /translation="ILGTVAGVAIARSRAVDDVEERARGAAASAAALLEIEVRQVAAG
                     LGGAAAVVGPDGDLDVEVFRAFASDPLGGEEVRELALIGIVSGGERAEFEARTGRPIS
                     VLDDRGIVAAPPAPVHHPVLSVQPDVGDGAAVGFDFGADRARRAVLEAAQRTRAAAVG
                     DVTDVAGGVGPVVPVVRPLLDGDDVVRGFVATGIPVDQLEATIDTASVSGAPVALVAG
                     ETLIAGDVLRHPDEATVAEVDVPAGSWRVLVGPGPEPDLTVSWLVAGGGGVAILAMAA
                     LVFVTERHQRRLARANALLATNEERSRAVQAVAGRLARALGGSDVATALVDHLPAAVG
                     ARSAVVATVDSAGRLGIIERDPGVGAGEGDGGATEVRRLAVPVTGSVVEDALVQRTPA
                     WLSSPLGWKGDDVATVLAGDGQALAVLPLLAEDVTGVLAVAYPRFHIFGDDEQELLQT
                     VGVLAARALARGRRYDAEHETAVAFQRTALPDALPVVAGATIAARYRPATHGATVGGD
                     WYDALLLDDDHVLLVVGDVVGHGMVAAAAMGRLRTAFQTIVRFTADPGLILQAINQQV
                     LSIPDAFCTTVVCVVLHLGTGELVWSRAGHPPPLVLDGDGRALLDAPCLPPLGVVDDA
                     VAPVHHRVLSAGDVVVLYTDGLVERRTEALDQGFRRLGAVAAQLADLDPEELSDALVE
                     ALVPSEEQTDDLAVLVVRYDGRGSGGG"
BASE COUNT          695 a         1874 c         1854 g          637 t
ORIGIN      
        1 gcgacctcgg gttcctggag ttgttcggct gcgcctcaca actcccatct gacgcaagca
       61 ccgggtgaca acgcggcgcc acggcgcgat ggtcgcgtga gcgggcggtc ccgtgggggg
      121 accgccgcga ccaaggggga cagggatggc cgacaacgcg tcgcgcatcg aggagctcta
      181 cggggccttc ggccgaggag acctggatgc ggtcctgggg gcgatggacc cgcagatcga
      241 gtggaacgaa gccgagcacg tgaccttctg gaccggtgcg ccgttcgtgg gtcccgacgc
      301 ggtggtgcag ggcgtcttcg cccgcatccc cgagacgttc ggggacacct tccgcatcga
      361 gccggagcgc ttcgtcgact gcggctccac cgtgctggtc caggccaggt accacggcgt
      421 cgcgcaggcg acgggcaagg agatcgacgt ccaggtcgcc cacgtgtggg acctcggcga
      481 cgacggcaag gtgacccggt tccagcagta caccgacacc tgggccttcg ccgaggcgac
      541 cggggagacc cccgaagcca gctgatgtcc ggctactgga gctcgatgct ccagtagccg
      601 tcggggcggc ggagcagggc ggtgcgccac ggcccctcgc ccaggtcgag cgccgacagc
      661 acccacacgt ccttgtccct ggccgcggcc gcccgcgccg cggtggcgag gaaggcgtgg
      721 tgcccgccct tggtgccgcc cggcgcgcac agcacggcgg cggtgccccg gtactcctcg
      781 aagcgctcct gctgggactc cggcagcgac cacttgagcg acgcgtcggc caggaccacc
      841 gtgtcgtcga ccagctcgcg cgccgcggcg accacctcgt cgagctgatc gaggtcgggc
      901 tcggcccgcc gccaacccgc ggccaggccg gcgtcgacga agaggatctt gctcatcggg
      961 gcgcgagcgc ctcgagggca tcgcggtcgg gctcgcccat gcgggccgac ggcttcagcg
     1021 gctgcaggca gacatgtgtc gcgccggcgt cgacgtgctc ggcgacgcgc gcctcgatct
     1081 cgtccgccgt gccccacggg accagcgcgt cgatgaggcg atcggagccc ccgtcggcca
     1141 ggtcgctctc gtcgaagccg aggcggaacc agttcttgaa gtagttcggc aggccgatgt
     1201 acacgccgag cgcggcgcgc gccgtggccc gggcctcggc cgcgtccgtg cagagcacca
     1261 ccttctgctc cacgcagagc atcttgtcgt cgccgaggac ggccttggcc tgggcggtgt
     1321 gctcgggggt ggtccagtac gggtgggcgc cgtcggctgc agtggcggcg agctcgagca
     1381 tcttcgggcc gagggcggcg atcaccgtct tcggtggcgt tgcgggcgcc ggggccatgt
     1441 acatcgagtc ggccatgccc tggaggtgct cgcgcatcga ggtgaggggc ttggagtagt
     1501 cgagcttgcg caccccggcc acgatcggcg cgtgggagac gcccgcgccg aggaggaacc
     1561 ggccgccgga ctgctcggcc agggtcagct gcgcctggcg catcgatccg gggtggcggt
     1621 tgaagatgtt ggcgatgccg gtggccacga ccagcgacga cgtgttcgac agcaggtggg
     1681 cggcgtgcgc gaacgggtcg cggcccaggg tctcggggat ccacagagcg ccgtagccca
     1741 gcgactccag ccactgcgcg aactcgacgg cggcctccac cggcatggca tcggtgggcc
     1801 accagatgcc ccgcggaccg atgcggtcct cgatgggggt gctggcgtca ggggtctccg
     1861 tggggctcat gggcggccat cctgccaccg gcaccggggc ccgacgcgcc ttcctgccgg
     1921 gtcacaccgg tgcgctaccg tcgtcggacc acgccgccga gccacagggg aagaccgtgt
     1981 tccgagcgtt gctgctcgcg ccgcccggcg caggcaaggg cacccagggt cagcgcctcg
     2041 ccgaccgcca cggcgtcccc cacctcgcca ccggagacct cctgcgcgac cacgtgtccc
     2101 gcggcaccga cctgggtcag caggccaagt cccacatgga tgccggccgc ctcgtccccg
     2161 acgagctggt catcgacatg gtgctgcgca agatcggcgg cgacggcgag ggcgagatcc
     2221 tcgaggactt cctcctcgac ggcttccccc gcaccctggc ccaggctcgc ttcgcctacg
     2281 agtgggggtt gcagttcagg cgcaccttcc acgccgtgat catgctcgag gtgcccgagg
     2341 acgagctcgt ccgccggctc gtgcagcggg gccaggagca gggccgcagc gacgacaccg
     2401 aggacgtcat ccgcaatcgc ctgacggtct acgagcagaa caccgcgccg ctggtcgact
     2461 tctacgagtc ccgcggcatc ctcgtccggg tcgacggcac cggcacggtc gacgaggtca
     2521 ccgcgcggat cgaggtcggc ctggagccga tggtgccggc gtgagccgcg agaacctggc
     2581 gggcgcccag cgcgccctgg ctgcggtcat cgccgtgggc atcctggtca tggccgcgat
     2641 cgccggtcgg tccatcttcg ggccctggga catcgtggtg cacggctacg tcggcaacgg
     2701 cctcttcgtg ctcgccgtcg tcaacctcgc cctcgccatc taccggcgac ccggcaccgc
     2761 cgagctcacc gtcgccgccc tcatcggcat gctcaccttc gcccaggtcg gcctcggcta
     2821 cgtcgggcgt gagcagctcg aagccgccgc ctggcacatc cccaacggcg tcctcctcat
     2881 ggcgctcagc acctaccagt gggcctcgct gcaggcccgg gctgccgtct catccgccac
     2941 ctgaacctcg gccgtcgtag cggaccacca ggacggcgag gtcgtcggtc tgctcctcgg
     3001 atggcaccag ggcctcgacg agggcgtcgc tgagctcctc ggggtcgagg tcggccagct
     3061 gcgccgccac ggcaccgagc cggcggaagc cctggtccag cgcctcggtg cggcgctcca
     3121 ccaggccatc ggtgtagagc accacgacgt cgccggccga gagcacccgg tggtgcacgg
     3181 gcgcgacggc gtcgtcgacc acgccgagcg ggggcaggca gggggcatcg agcagcgcac
     3241 gcccgtcgcc gtcgagcacg agcgggggcg ggtgcccggc tcgggaccag accagctcac
     3301 cggtgccgag gtgcagcacg acgcacacca cggtggtgca gaacgcgtcg gggatcgaca
     3361 gcacctgctg gttgatcgcc tgcaggatca agccgggatc ggccgtgaac cggacgatcg
     3421 tctggaaggc cgtgcgcagg cgacccatgg cggcggcggc gaccatgccg tggccgacca
     3481 cgtcgccgac caccagcagg acgtggtcgt cgtcgaggag cagggcgtcg taccagtcgc
     3541 caccgacggt cgccccgtgg gtcgccggcc ggtagcgggc ggcgatggtg gcgccggcca
     3601 cgaccgggag cgcatccggc agcgcggtcc gctggaacgc cacggcggtc tcgtgctcgg
     3661 cgtcgtagcg gcgaccgcgg gccagggccc gggcggcgag cacgccgacc gtctgcagca
     3721 gctcctgctc gtcgtcgccg aagatgtgga accgggggta cgccacggcc agcacgccgg
     3781 tgacgtcctc ggcgagcagc gggaggacgg cgagcgcctg gccgtcgccg gcgagcaccg
     3841 tggccacgtc gtcgcccttc cagccgagcg gcgacgacag ccacgccggg gtccgctgga
     3901 cgagggcgtc ctcgaccacc gacccggtga cgggcaccgc cagccggcgg acctcggtgg
     3961 cacccccgtc gccctccccc gccccgacac ctggatcgcg ctcgatgatc ccgagccgcc
     4021 cggcgctgtc caccgtggcg accaccgccg atcgggcccc gaccgcggcg ggcaggtggt
     4081 ccaccagggc ggtggcgacg tcgctgcccc cgagggcgcg ggcgagccgg ccggcgaccg
     4141 cctggacggc gcggctgcgc tcctcgttgg tggccagcag ggcgttggcc cgggccagcc
     4201 ggcgctggtg acgctcggtc acgaagacca gcgcggccat ggccaggatc gccacgccac
     4261 cgccaccggc cacgagccac gagaccgtca ggtcgggttc ggggccgggt cccaccagga
     4321 cccgccacga gccggccggg acgtcgacct ccgccaccgt cgcctcgtcg gggtggcgca
     4381 ggacgtcgcc ggcgatcagc gtctcgccgg ccacgagcgc caccggggca cccgagacgg
     4441 aggccgtgtc gatcgtcgcc tcgagctggt ccacggggat cccggtggcg acgaagcctc
     4501 gcaccacgtc gtcgccgtcg agcagcggcc gcaccacggg caccacgggg ccgaccccgc
     4561 ccgccacgtc ggtgacgtca ccgaccgccg cggcgcgcgt ccgctgggcg gcctcgagga
     4621 ccgcccgccg ggcgcggtcg gcgccgaagt cgaagccgac cgcggcgccg tcaccgacat
     4681 cgggctgcac gctgaggacg gggtggtgca ccggcgcagg cggggcagcg acgatccccc
     4741 ggtcgtcgag caccgagatc ggacgtccgg tccgggcctc gaactcggcg cgctcgcctc
     4801 cggagacgat gccgatcagg gcgagctccc gcacttcctc cccgcccaac gggtcgctgg
     4861 cgaaggctcg gaagacctcg acgtcgaggt cgccgtccgg cccgacgact gcagcggcgc
     4921 caccgagccc ggccgccacc tggcggacct cgatctcgag cagggcggcg gcgctcgccg
     4981 cggcgccgcg ggcccgctcc tcgacgtcat cgaccgcccg gctccgagcg atcgccacgc
     5041 cggcaaccgt gccgaggatc
//
LOCUS       DATISU010000392         6412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_173150, whole genome shotgun sequence.
ACCESSION   DATISU010000392 DATISU010000000
VERSION     DATISU010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173150"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..757)
                     /locus_tag="VLR27_15565"
     CDS             complement(110..757)
                     /locus_tag="VLR27_15565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HSP04928.1"
                     /translation="MPVDGPQYPVNLVLRGRSVLVVGGGPVATSKVEGLLDGGADQIL
                     VVAPDVTDDLAALEAGGIVAIERRRYASPEAAEHRLVVTATSEPDVNHQVFLDADAAG
                     VWVNSADDPANCTFTLPARVRRGTLLVTVATGGHSPALATWLRRRFEDEFGPEYDALV
                     ELLSAERESIKAQGRSTEGLKWQEALDAGLLDLLRADRRTEALELLRSCLSSSSG"
     gene            complement(760..1410)
                     /locus_tag="VLR27_15570"
     CDS             complement(760..1410)
                     /locus_tag="VLR27_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HSP04929.1"
                     /translation="MAERGGKRIPDATVARLPIYLRSLLELGPQTPTVSSEQLAESAG
                     VNAAKVRKDLSYLGSYGTRGVGYDVEYLQAHMRRALGMTQDWPVVIVGIGNLGAALAN
                     YGGFGNRGYPVAALVDASPDVVGRSFGDLVVRHADELADVVAEEHIAIGVIATPAPAA
                     QAVADELIAAGVRSILNFAPTVLNVPPEVSLRKVDLAVEMQILSFYQQHGPASAAE"
     gene            complement(1461..2978)
                     /locus_tag="VLR27_15575"
     CDS             complement(1461..2978)
                     /locus_tag="VLR27_15575"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP04930.1"
                     /translation="MNLAEICADHPATSVALISRGKETTYGELREQVGRLRGGLVQAG
                     IQPGERVAIVAANNWYFVASWLAILGVGAVAVPINPASPGPEVTRELADVGVVGVIVG
                     PTGKRVLPDIDRASVPDLRLVVQSDPGDLVVEGAVRLDELMAVEPTPIVPRDADDLAV
                     LMFTSGTAGFPRAAKLSHGNLLANLDQLQANPYREQRSHDITFGVLPFFHIFGLNVVL
                     DMALRTGGAVLAVERFDPESALESVKKHEVTIVAGAPAMWSAWAHLPGADGDALRTVR
                     IAGSGAAKLDPAVRTLVQERFGLTIDEGYGLTEASPVVTTATGIPAPPGSIGAPLPGV
                     KVRLTDSEGRDVLVGDVGEIRVQGPNVFQGYWDDEDITSQTLTPDGWLKTGDLAVVDD
                     DGFLFIVDRLKDLIIVSGFNVYPAEVESILAEHPGVAAAAVVGVQHPHSGEAVKAYVV
                     AAEGASLEEDEIIAFAEQRLARYKCPQKVWFVDELPKGLGGKVRKHELRGTRTSH"
     gene            3043..4026
                     /locus_tag="VLR27_15580"
     CDS             3043..4026
                     /locus_tag="VLR27_15580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP04931.1"
                     /translation="MHDGIVTEGLTRLFPGGVGLADLSLEVASGEVLALLGPNGAGKT
                     TTVRLLNGVLRPDRGHARVLGLDPATEGDELRRRTGVLTEHAGLDDRLTARENLHYAA
                     RIRGDTGPAVEARIGELLDRFGMGERADQPVRGASTGQRKRLGLARSLLHDPEVLFLD
                     EPTSGLDPTATRDVVELIGSLATEHGRTIVLATHFLGEAGRLADRMAVLDRGELVAYG
                     RPGDLAADLWDGLEAAIDLGARATTEQLDAIAAVAGVHTVEPSPTGAVLRVAGRDVLP
                     QVVARLVGVGADVFAAVPRPPTLEDVYFEIEARRAASAGLPPAPDHQELAT"
     gene            4023..4835
                     /locus_tag="VLR27_15585"
     CDS             4023..4835
                     /locus_tag="VLR27_15585"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSP04932.1"
                     /translation="MTRTDWTAVWGVVAKDLRAVVRSKSVVIPMLAVPVLLMVFLPGA
                     IGLAARAAPGVQVERLLGDVPGDLARPILDLPVEEQLIVLVNGYLLAPLFLIVPLMVS
                     AVLAADAFAGEKERKTLESLLHSSIRDRDLFLAKFLSAFLPALAVSWLGFLAFVIVAN
                     GVAWPVMGRIFVPTRLWGVMILWVAPAVATLGLAVMVRVSARTHTAQEANQLGGAVIL
                     PLIFLAVGQSTGLLLVTLPIAIGIGAVIWAVALLLLRGGVKRFTRDALVAQV"
     gene            4882..5664
                     /locus_tag="VLR27_15590"
     CDS             4882..5664
                     /locus_tag="VLR27_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04933.1"
                     /translation="MSFDLAAYKESTDRLDVGGIDFDAFREQPLAPEHLRCLRYMHDV
                     ELHTSCYLRNLLNTRAHHDPEITTFLTLWNHEELWHGEALARVLAAHDEPGGAPRVAS
                     MRQRLGWRITASPLLWMGFSAMTSHFLAIHMTFGVINEWTTQAGYVRLAVGAQHPVLD
                     ELLRRIMRQEGTHIAFYRSHAVERLEASERAQRTTRRAVRALWSPVGASVMPEAETRH
                     LVRTLFSGPDGRAVADRIDRRVDALPGLDGLDLMAGTMSRYG"
     gene            complement(5819..>6412)
                     /locus_tag="VLR27_15595"
     CDS             complement(5819..>6412)
                     /locus_tag="VLR27_15595"
                     /inference="COORDINATES: protein motif:HMM:NF042701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HSP04934.1"
                     /translation="GPDNGLLAPAVSMVGGATRAVSLTNTDFHLPAPGPTFDGRDVFA
                     PVAAALCTGTDLAELGDLVEATSLVPGLIPVMREEDGALVCDVLWIDRFGNCQLNVDP
                     DDLDGWGDRIGLRIGGQLRVARRVDAYARIKEGEVGLVVDSYGLLAVALDRRSAHAEL
                     GLGTGQEVRLERLDGDEGPVGVTSPVSLGSRPQGGRP"
BASE COUNT          858 a         2350 c         2288 g          916 t
ORIGIN      
        1 acggcctcgg acacgtgctc cttggtgagc agcgcgtgca gcgtcttgcc gagcgcagcg
       61 tcgccgacgg tcatcgcctc gagcaccccg aggggggccg tgcggtggtt caccccgatg
      121 acgacgacag gcatgatctc agcagttcca gcgcttcggt tcggcggtcg gcacgcagca
      181 ggtcgagaag cccggcatcg agggcctcct gccacttgag tccctcagtt gaacgaccct
      241 gggccttgat ggattccctc tccgcggaga ggagctccac cagggcatcg tattccggac
      301 cgaactcgtc ttcgaatcgc ctccgcagcc aggtggccag ggcggggctg tgaccccccg
      361 tcgcgacggt caccaggagc gtcccgcgcc ggacgcgcgc cggcagcgtg aaggtgcagt
      421 tggccgggtc gtcggccgag ttcacccaca cgccggcggc gtcggcgtcg aggaacacct
      481 ggtggttgac gtcgggctcg ctcgtggcgg tgacgacgag gcggtgctcg gcggcctcgg
      541 ggctggcgta gcgacggcgc tcgatcgcca cgatcccgcc ggcctcgagg gctgcgaggt
      601 catcggtcac atccggcgcg acgaccagga tctggtccgc tcccccgtcg agcaacccct
      661 ccaccttcga cgtggcgacc ggcccgcccc cgaccacgag caccgaccgg ccccgcagca
      721 ccaggttcac cgggtattgc gggccgtcga ccggcatcgc tactcggccg ccgaggccgg
      781 gccgtgctgc tggtagaagg acaggatctg catctccacg gcgaggtcga ccttgcggag
      841 cgagacctcg ggcggcacgt tcagcaccgt cggggcgaag ttcaggatcg agcgcacgcc
      901 ggccgcgatg agctcgtcgg ccaccgcctg ggccgccggc gccggcgtgg cgatgacgcc
      961 gatggcgatg tgctcctcgg ccaccacgtc ggcgagctcg tcggcgtggc ggaccacgag
     1021 gtcgccgaag ctgcgcccga ccacgtcggg gctggcgtcg accagggcgg ccaccgggta
     1081 gccgcggttg ccgaagccgc cgtagttggc gagggcggca ccgaggttgc cgatgccgac
     1141 gatgacgacg ggccagtcct gggtcatgcc gagcgcccgg cgcatgtggg cctgcaggta
     1201 ctcgacgtcg tagcccacgc cccgggtgcc gtagctgccg aggtaggaca ggtccttgcg
     1261 gaccttggcc gcgttcacgc cggcggactc ggcgagctgc tcgctgctca ccgtgggcgt
     1321 ctgcgggccg agctcgagca ggctgcgcag gtagatcggc aggcgcgcca cggtggcatc
     1381 ggggatgcgc ttgccaccac gctcggccac ggcacgaggg tagggctcgt tcacacgttc
     1441 acaaagtccg cgcggcggcc tcagtgagag gtccgcgtgc cccgcagctc gtgcttgcgg
     1501 accttgccgc cgaggccctt cgggagctcg tcgacgaacc acaccttctg cgggcacttg
     1561 taccgggcga ggcgttgctc ggcgaacgcg atgatctcgt cctcctcgag cgaggccccc
     1621 tcggcggcca ccacgtaggc cttcaccgcc tcgccggagt gggggtgctg cacgcccacc
     1681 acggcggccg cggcgacgcc cgggtgctcg gccaggatgc tctcgacctc ggccgggtac
     1741 acgttgaacc cggacacgat gatgaggtcc ttcaaccggt cgacgatgaa gaggaacccg
     1801 tcgtcgtcca ccaccgccag gtcgccggtc ttcagccacc cgtcgggggt gagcgtctgg
     1861 gacgtgatgt cctcgtcgtc ccagtagccc tggaacacgt tcgggccctg cacccggatc
     1921 tcgccgacgt cgccgacgag gacgtcgcgg ccctcggaat cggtgagacg caccttcacg
     1981 ccgggcagcg gcgcaccgat gctgccgggg ggcgccggga tgccggtggc ggtggtgacg
     2041 accggcgacg cctcggtgag cccgtagccc tcgtcgatcg tcaggccgaa gcgctcctgg
     2101 accagggttc ggaccgccgg gtcgagcttc gccgcccccg aaccggcgat gcggaccgtg
     2161 cgcagcgcgt cgccgtcggc tcccggcagg tgcgcccacg ccgaccacat ggcgggtgcc
     2221 ccggcgacga tggtgacctc gtgcttcttc acggactcga gggcggactc ggggtcgaag
     2281 cgctccaccg cgagcacggc gccgccggtg cgcagggcca tgtcgagcac cacgttgagg
     2341 ccgaagatgt ggaagaacgg cagcacgccg aaggtgatgt cgtggctgcg ctgctcccgg
     2401 tacgggttgg cctgcagctg atcgaggttg gccagcaggt tgccgtggct gagcttggcg
     2461 gcccggggga agccggcggt gcccgaggtg aacatgagca cggcgaggtc gtcggcgtcg
     2521 cgggggacga tcggcgtcgg ctcgacggcc atgagctcgt cgaggcgcac ggccccctcg
     2581 accacgaggt cgccgggatc ggactgcacc acgaggcgca ggtcgggcac ggacgcccgg
     2641 tcgatgtcgg gcagcacccg cttgccggtg gggccgacga tgacgccgac gacgccgacg
     2701 tcggccagct cgcgggtgac ctcgggcccg ggactggccg ggttgatcgg cacggcgacc
     2761 gcgccgacgc cgaggatggc gagccagctc gccacgaagt accagttgtt ggcggccacg
     2821 atcgcgaccc gctcgccggg ctggatgccg gcctggacga gaccgccccg caggcgcccg
     2881 acctgctcgc gcagctcgcc gtaggtggtc tccttgcccc gggagatgag cgccacgctc
     2941 gtcgctgggt ggtcggcaca gatctcggcg aggttcacgc cgggcagttc agcacgtggg
     3001 cggcgtgcga tgctgccgcg tggtccccgg tagacatgcg acgtgcacga cgggatcgtc
     3061 accgagggcc tgacccgcct gttccccggc ggcgtcggct tggccgatct gtcgctggag
     3121 gtcgcgtccg gcgaggtgct ggcgctgctc ggacccaacg gcgccggcaa gaccacgacg
     3181 gtgcggctcc tcaacggcgt gctgcggccc gaccggggcc acgcccgggt cctcggcctc
     3241 gacccggcca ccgaaggtga cgagctgcgc cggcgcaccg gggtgctcac cgagcacgcc
     3301 gggctcgacg accggctcac cgcccgcgag aacctgcact acgccgctcg catccggggc
     3361 gacaccggac cggccgtgga ggcccgcatc ggcgagctgc tcgaccgctt cggcatgggc
     3421 gagcgcgccg accaaccggt gcggggcgcc tccaccggcc agcgcaagcg gctggggctg
     3481 gcccggtccc tgctccacga ccccgaggtc ctgttcctcg acgagcccac ctccgggctc
     3541 gaccccaccg ccacgcggga cgtggtggag ctgatcggct ccctggccac cgagcacggc
     3601 cgcaccatcg tgctcgccac ccacttcctc ggggaggccg gccggctcgc cgaccgcatg
     3661 gccgtgctcg accgcggcga gctggtcgcc tacgggcgtc ccggcgacct ggcggccgac
     3721 ctgtgggacg gcctcgaggc cgcgatcgat ctcggggctc gggccaccac cgagcagctc
     3781 gacgccatcg ccgccgtcgc cggggtccac accgtcgagc catcgccgac gggcgccgtc
     3841 ctgcgcgtgg ccgggcggga cgtgctcccg caggtggtcg cccgcctcgt cggcgtgggg
     3901 gcggacgtgt tcgccgccgt cccccggcca ccgaccctcg aggatgtgta cttcgagatc
     3961 gaggcgcgac gggcggcctc ggccgggctg ccaccggccc cggaccacca ggagctggcg
     4021 acgtgacccg caccgactgg accgccgtgt ggggcgtggt ggccaaggac ctgcgcgccg
     4081 tcgtgcgctc caagtccgtc gtcatcccga tgctggccgt gcccgtcctg ctcatggtgt
     4141 tcctgcccgg tgccatcggc ctcgcggccc gggccgcgcc gggcgtgcag gtcgagcgtc
     4201 tcctcggcga cgtgcccggc gacctcgccc ggccgatcct ggacctcccg gtcgaggagc
     4261 agctcatcgt gctggtgaac ggctacctgc tcgccccgct gttcctgatc gtgccgctca
     4321 tggtgtcggc cgtgctggcc gccgacgcct tcgccgggga gaaggagcgc aagacgctcg
     4381 agtcgctgct ccactcctcc atccgcgacc gcgacctgtt cctggccaag ttcctctccg
     4441 cgttcctgcc cgccctcgcg gtgtcctggc tcgggttcct cgccttcgtg atcgtcgcca
     4501 acggcgtggc ctggccggtg atgggccgga tcttcgtgcc gacgcgcctg tggggcgtga
     4561 tgatcctgtg ggtcgcgcct gcggtcgcca ccctcggcct ggccgtgatg gtgcgggtct
     4621 cggcccggac ccacaccgcc caggaggcca accagctcgg cggcgccgtg atcctgcccc
     4681 tgatcttcct cgccgtcggc cagtccaccg gcctgctgct tgtgacgctg ccgatcgcca
     4741 tcggcatcgg cgccgtcatc tgggccgtgg ccctgctgct gctccggggc ggcgtcaagc
     4801 gcttcacccg cgacgcgctc gtcgcccagg tctagaccga acccggcaat ccggacgctc
     4861 ccggcgttcc gaacaccagg cgtgagcttc gacctggccg cctacaagga atccaccgac
     4921 cggctcgacg tcggcgggat cgacttcgac gccttccgcg agcaaccgct cgcccccgag
     4981 cacctccgct gcctgcggta catgcacgac gtcgagctcc acacgtcctg ctacctgcgg
     5041 aacctgctga acacccgggc ccaccacgac ccggagatca ccaccttcct caccctctgg
     5101 aaccacgagg agctctggca cggcgaagcc ctcgcccggg tgctcgccgc ccacgacgaa
     5161 cccgggggcg cgccccgtgt cgcctccatg cgccagcgcc tcgggtggcg catcaccgcc
     5221 agccccctgc tgtggatggg cttctcggcg atgacctccc acttcctcgc catccacatg
     5281 accttcggtg tcatcaacga gtggacgacg caggccggct acgtccgtct cgccgtcgga
     5341 gcccagcacc ccgtgctcga cgagctgctc cgacgcatca tgcgacagga gggcacccac
     5401 atcgccttct accgctcgca tgccgtggag cgcctcgagg cgagcgaacg agcgcagcgc
     5461 accacccgcc gcgccgtccg tgcactctgg agcccggtcg gcgcgtccgt catgcccgag
     5521 gcggagaccc gccacctggt gcggacgctg ttctccggac ccgacggtcg agccgtcgcc
     5581 gaccgcatcg accggcgcgt cgacgccctc ccggggctgg acggcctcga cctgatggcc
     5641 ggcaccatga gccgctacgg ctgagccgtg gcgttctggc ctgcatccgt gccacctgac
     5701 ggtcatggat gtaggccaga acgaggctgt gggggctagg tcaggaagac gagctggagg
     5761 gtggcggcgc cgatgatcag cagcagcagg atcgccatga cgatggtggt tccgggcctc
     5821 atggccttcc tccctgggga cgtgatccga ggctgaccgg cgaggtcacc ccgacgggac
     5881 cctcgtcgcc gtcgaggcgc tcgaggcgca cctcctggcc ggtgccgagg ccgagctcgg
     5941 cgtgcgcgga gcgccggtcg agggcgacgg cgagcaggcc gtaggagtcc acgaccaggc
     6001 cgacctcgcc ctccttgatg cgggcgtagg cgtcgacccg gcgagccacg cgcagctggc
     6061 cgccgatgcg caggccgatg cggtcgcccc agccgtcgag gtcgtccggg tcgacgttga
     6121 gctggcagtt gccgaagcgg tcgatccaga gcacgtcgca cacgagggcg ccgtcctcct
     6181 cgcgcatgac ggggatcagg ccggggacca gcgaggtcgc ctcgaccaga tcgcccagct
     6241 cggcgaggtc ggtgcccgtg cacagggcgg cggcgacggg ggcgaacacg tcacgaccgt
     6301 cgaaggtcgg acccggcgcc ggcaggtgga agtcggtgtt ggtgagcgag acggctcggg
     6361 tggcgccgcc caccatggag accgccgggg cgagcaggcc gttgtcgggc cc
//
LOCUS       DATISU010000393         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_638137, whole genome shotgun sequence.
ACCESSION   DATISU010000393 DATISU010000000
VERSION     DATISU010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638137"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VLR27_15600"
     CDS             <1..588
                     /locus_tag="VLR27_15600"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSP04935.1"
                     /translation="VRATKTGFLGSAGTVDVVARVAEAGRLANLVVDPVLVASTGAAL
                     FPPEVQEALVARLLPQARVITPNRTEAGLLVGRALPTLADMEAAAVELLDLGCEAVVV
                     KGGDAEDEGDQAVDVLAVAGRTERIALARVDTPNDHGSGCSFAAATAARLALGDDVPE
                     AVRGAKAFVHRALLGARDWRLGGGHGPIDAFGWDS"
     gene            687..1547
                     /locus_tag="VLR27_15605"
     CDS             687..1547
                     /locus_tag="VLR27_15605"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSP04936.1"
                     /translation="MPRVLITGCSSGFGLLTAVELAGRGHEVVATMRDPARDGDLRRA
                     CEEAGTSVHVVALDVHDEGSVASGVGEAVDLLGGLDVVVNNAGIEVRGPVELVSDDEV
                     ALQFETNVFGLLRVVRATVPHLRDSDAGVLVDMSSVAGVIARPFAGIYAASKHAVEAI
                     TEALHFELGLSGIRVHSIQPGQFPTELGANTVTAAAFTPDHDGYWAIAEELESRAKGL
                     GGDGPADPALVARTVADVIEDEAAPLRTPVGADAELIVAARHGHTFEEYEVLMRSALD
                     HWEGYRRERP"
     gene            complement(1526..1795)
                     /locus_tag="VLR27_15610"
     CDS             complement(1526..1795)
                     /locus_tag="VLR27_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04937.1"
                     /translation="MGRPTDAATKRARTRRRNARRAATRATLQLVVALFGGLLAGGLV
                     ITAALPGTAGPIPATATTAAAVVVAVATLVAARRLRPVDQGRSRR"
     gene            1856..>2349
                     /locus_tag="VLR27_15615"
     CDS             1856..>2349
                     /locus_tag="VLR27_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HSP04938.1"
                     /translation="MTATMKANDQCFCGSGRKFKRCHKASFEPVRQGAISPLRPVPDS
                     IELPTYVVEGRVDRWDEPAVKSPDVIERMRRTGAEAARILALAGAAVRPGTTTDELDR
                     IVHEATIDAGAYPSPLLYGGPDNPFPKSVCTSVNEVICHGIPDDRELVDGDIVNIDVT
                     IFREG"
BASE COUNT          311 a          862 c          872 g          304 t
ORIGIN      
        1 gtgcgggcca ccaagaccgg cttcctcggc tcggcgggca ccgtggacgt ggtcgcacga
       61 gtcgccgagg ccggccggct ggcgaacctc gtggtcgatc cggtgctcgt ggcctcgacg
      121 ggagcggccc tgttcccgcc cgaggtccag gaggcgctgg tcgccaggct gctgccccag
      181 gcgcgggtga tcacgcccaa ccgcaccgag gccgggctcc tcgtcggtcg agccctgccg
      241 acgctggccg acatggaggc ggccgccgtc gagctgctcg acctcgggtg cgaggcggtc
      301 gtggtcaagg gcggcgacgc cgaggacgag ggcgaccagg ccgtcgacgt gctcgccgtc
      361 gccgggcgga ccgagcgcat cgccctggcc cgggtcgaca cccccaacga ccacggttcc
      421 ggctgctcgt tcgccgcggc gacggccgct cgcctggcgc tcggtgacga cgtgcccgag
      481 gccgtgcgtg gagcgaaggc gttcgtgcac cgggcgctcc tcggcgctcg cgactggcgc
      541 ctcggtggcg gccacgggcc catcgacgcc ttcggctggg acagctgacg caacaccagc
      601 actgccagtt cgggacgacc gcacagggac ccgcctgaac cgttctggca gtgctcacgt
      661 cacgccggga ccgctagacc gtcggcgtgc ccagggtcct gatcaccggt tgctccagcg
      721 gcttcggcct cctcacggcc gtggagctgg ccgggcgggg ccacgaggtc gtggcgacca
      781 tgcgcgaccc cgcccgggac ggcgacctcc gccgggcctg cgaggaggcc ggcacctcgg
      841 tgcacgtggt cgccctcgac gtgcacgacg agggctccgt cgccagcggt gtgggggagg
      901 cggtggacct gctcggcggg ctggacgtgg tggtcaacaa cgccggcatc gaggtgcggg
      961 gacccgtcga gctggtgtcc gatgacgagg tcgccctgca gttcgagacc aacgtgttcg
     1021 ggctgctgcg ggtggtgcgg gccaccgtcc cgcacctgcg ggactcggac gcgggtgtcc
     1081 tcgtcgacat gagcagcgtg gccggtgtca tcgcccggcc cttcgccggg atctacgccg
     1141 cttccaagca cgccgtggag gcgatcaccg aggccctcca cttcgagctc ggcctgtccg
     1201 gcatccgggt ccactcgatc cagccggggc agttccccac cgagctgggc gccaacaccg
     1261 tcacggcggc ggccttcacc cccgaccacg acggctactg ggcgatcgcc gaggagctcg
     1321 agtcccgcgc caaggggctg gggggcgacg ggccggccga ccccgccctc gtcgctcgga
     1381 ccgtggccga cgtgatcgag gacgaggcgg cgccgctgcg gaccccggtc ggcgccgacg
     1441 ccgagctgat cgtggctgcc cgtcacggcc acacgttcga ggagtacgag gtgctgatgc
     1501 gcagcgcgct ggatcactgg gagggctacc ggcgcgaacg cccctgatcg accggccgga
     1561 ggcgacgggc cgccaccagc gtcgccacgg caaccacgac ggcggccgcg gtggtggcgg
     1621 tggccgggat cggcccggcg gtgccgggca gggcggcggt gatgaccaga ccgccggcga
     1681 gcagaccgcc gaacagggcg acgacgagct gcagcgtggc ccgggtggcg gcgcgacggg
     1741 cgttgcgccg tcgggtccgg gctcgcttgg tggcagcatc ggtggggcgt cccacggctc
     1801 cgaccgtacg catccgcccg gggcggccgg tggatccggc gttaccctgg gggccgtgac
     1861 ggccacgatg aaggcgaacg accagtgctt ctgcggcagc ggccgcaagt tcaagcgctg
     1921 ccacaaggcg agcttcgagc ccgtccggca gggggccatc tccccgctgc gccctgtccc
     1981 cgacagcatc gagctgccca cctacgtggt cgagggccgg gtcgaccgct gggacgagcc
     2041 cgccgtgaag tcgcccgacg tcatcgagcg catgcggcgc accggtgccg aggccgctcg
     2101 catcctcgcc ctcgccggtg ctgccgtccg gccgggcacc accaccgacg agctcgaccg
     2161 catcgtccac gaggccacca tcgacgcggg cgcctacccc agcccgctgc tctacggcgg
     2221 ccccgacaac ccgttcccga agtcggtctg cacgtcggtg aacgaggtca tctgccacgg
     2281 catccccgac gaccgcgagc tggtcgacgg cgacatcgtg aacatcgacg tcaccatctt
     2341 ccgcgaggg
//
LOCUS       DATISU010000394        10814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_313330, whole genome shotgun sequence.
ACCESSION   DATISU010000394 DATISU010000000
VERSION     DATISU010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10814
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313330"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VLR27_15620"
     CDS             <1..1330
                     /locus_tag="VLR27_15620"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HSP04939.1"
                     /translation="AIATLGGGGALGAEGPSVYAGARIGPRLGRRLTSVLGPRGHRAL
                     TSAGAAAGVAAIFQAPATGVLFALETPFQRDVARRALIPALIAAATSYLTFVSIFGVH
                     SYLPVPRGEVRLRDEVFGAILLGLCAGITARGLAGLWGAAKGLSERVSPVVRLAAGAA
                     VSGLTLVAARGLVGTSFTLGPGVEMVAEIAVDPTIGLWVIVAAFALRALATSAALGAG
                     GVGGVFIPLVVQGLLLGRLVESLFTDAAPGLYPVIGLAAVLGAGYRTPLAAVMFVAET
                     TGQAQFVIPALVATAVSQAVMGEKSVASYQVTEREGMLERRLRLPVRNVVIDVPILHP
                     DQLVTDVIDTYGDAPPAQALPVCDDRYRGLLMLRDMAMALFEIGPDATVGQAMHDVPA
                     VRVDVPAVEAARIMGELDSAIIAVIDADDVAIGVVTALTMTGALDYDPDD"
     gene            1360..2406
                     /locus_tag="VLR27_15625"
     CDS             1360..2406
                     /locus_tag="VLR27_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HSP04940.1"
                     /translation="MKLGLSLGYWGRGPNPDTLDNVLAAESMGFDSMWTAEAWGSDAL
                     TPLAWYGSRTTTMKLGQSITQISARTPAATAMAMQTLQHLSDGRVICGLGVSGPQVVE
                     GWYGQPFAKPLARTREYVSILKQVWERGEPVTSEGPHYPLPYPADAEGSVGKGKALRS
                     ITHPHPTPIPVYLAAEGPKNLALAGEMADGWLPLWMSPDESSWYEAQIQKGMDRVPGK
                     SWDDFGVAATVPTLVLDDLDAAIDLMRPNLALYIGGMGAKGENYHYDVFARMNEGRYE
                     GTCEQIQELYLSGHKDEAIATVPAEMVDDICLVGSADRVRDRVQRWEDSIVDTLLIGG
                     PWQTVQAAGAALLD"
     gene            complement(2403..4682)
                     /locus_tag="VLR27_15630"
     CDS             complement(2403..4682)
                     /locus_tag="VLR27_15630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HSP04941.1"
                     /translation="MVSPPPEHDPGPQDDAVGVSVGEPETTAAGLPGVRHGLGHVLRR
                     VGIRRGARILRDLNQPGGVDCPGCAWPEAEDPSHAEFCENGMKAVAEEATRRRVDPTF
                     FERHPIAELAERSDWWLGQQGRITDPVHRPPGATHYQPISWDAAFAVIARHLQATSPD
                     RAVFYTSGRTSNEAAFLYQLFVRDLGTNNLPDCSNMCHESSGSALTPTIGIGKGTVSL
                     RDFDDADVILIVGQNPGTNHPRMLSSLERAKERGATIVAINPMPEAGLLGFRNPQRAR
                     GLLGSGTGLADHHLAIRVGGDLALFQLVNAALVAQGALDRGFVDDRVDGLDALASSLA
                     GIDHAARLEATGLTQADIDLLVDLLAGTQRFITCWAMGLTQHKGSVPTIREIVNTHLL
                     RGAIGLPGAGLCPVRGHSNVQGDRTMGIWEKPPAPFLDALAAEFGFDPPREHGLDTVH
                     AIRAMARGDVDVFVGMGGNFVSAAPDTEKTAAGLASCGLTVQVSTKLNKSHALCGEEA
                     LILPCLGRTEIDRTGGLVQSVTVEDSMGEVHATQGSVEPVSDHLRSEVAIVCALAEAT
                     LPDSTVPWSSFADDYARIRTRISRVVPGFERFEERIAGGARFALPNAPRDERSFETPS
                     GRAQLTVNEFEPIRIPEGRLLLQTVRSHDQFNTTIYGLDDRYRGITGGRRVVFCNAQD
                     LVDLGLTDGDVVDLVSEWPGDDDRRAPSFRVVEYPIARRTAAAYFPEANVLVPVDSVA
                     TTSNTPTSKSIIVRLERRS"
     gene            complement(4704..5534)
                     /locus_tag="VLR27_15635"
     CDS             complement(4704..5534)
                     /locus_tag="VLR27_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSP04942.1"
                     /translation="MEQLRGRVAAITGAGSGIGRALAVELARAGTHLALSDVDEVGLA
                     ETVSKAEGFGVKVTSARVDVADRGAVERWADEVVGDHGQVNLVFNNAGVALGATVEGA
                     RYEDLHWLMDINFWGVVHGTKAFLPHLKDSGAGHVVNISSVFGLLGIPSQSAYNAAKF
                     AVRGFTDALRMELEIEPCGVSATTVHPGGIKTNIAKNARMDDSVTAMADDPEQARRDF
                     ERMFMTTPEKAARQIIKAVQRNRRRALIGPDAKAIDFVSRLPAAVVQKVIVKGASRMR
                     "
     gene            complement(5546..6442)
                     /locus_tag="VLR27_15640"
     CDS             complement(5546..6442)
                     /locus_tag="VLR27_15640"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HSP04943.1"
                     /translation="MTITEPKPTTQTSNASRDVPTRRISFEEALRDLPKHFAEDGDII
                     GSHLIASLSSVFPDGEDFFVRSVRHYRSEITDPDLKRQVSGFIGQEAVHGREHREFND
                     RLDQLGYPTKAAERFTKKGLALRERIAPPISNLASTAALEHFTATLAEIILSDERARD
                     MIGHEEVKNLFLWHALEESEHKAVAFDVFKAVGGSERMRVWTMKFIRSGFVIGMGLQV
                     IVSVLLDPDARRHPVKLWRSFRKLKASPFLSRETWRTLKEYDDPDFHPDDRDTDELVS
                     RWRAELFGDEGTLNDKLATRAA"
     gene            complement(6439..7041)
                     /locus_tag="VLR27_15645"
     CDS             complement(6439..7041)
                     /locus_tag="VLR27_15645"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP04944.1"
                     /translation="MPRISEEQRAAARQRLIDATVAVAERDGVDGLTTRAIVTEAGVS
                     AGMFYGHFDSKEALLAAVVDHKTDELTTLVHAELELGTPMEGVVRALVDQLVRVLDLK
                     VLASFRTGSGTEDALAAQRRINHRIVDAFVPLLHESVDAGVVRPDIDPEAVVEFIDLV
                     IDGLNRRRALDGFVSSDDRLTAVIIAAFERYLLVPQEDPR"
     gene            7258..7476
                     /locus_tag="VLR27_15650"
     CDS             7258..7476
                     /locus_tag="VLR27_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HSP04945.1"
                     /translation="MALGACRGADPDLFFPDRGESLEPAKRICSECVVRDECLEHALA
                     SGERFGVWGGTSERERRRLRRTRRTAAA"
     gene            complement(7541..8899)
                     /locus_tag="VLR27_15655"
     CDS             complement(7541..8899)
                     /locus_tag="VLR27_15655"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HSP04946.1"
                     /translation="MGPADVVAALQDALPAESVVVDPDVVDGYRFDRAKTVVPGTPIA
                     LVRAGSTDEVAATMRVASRFGVGVVTRGAGTGLSGGSAAVDGCITLSTERMREVRIDA
                     GSMVAVAQPGALNVEVKAAARAEGLWYPPDPSSYEICSIGGNLATNAGGLCCVKYGVT
                     TDYVLGIEVVLADGTVTRLGGRTIKDVAGLDLKRLFVGSEGTLGVITEATLRLRPTPP
                     PSSTVVATFADPVAAGVAVAATMASLRPSALELMDQAAVAAVESVKPMGLDTSIGALL
                     LGQSDAGDATAARIAEHAEAAGATYVAVTDDPDEGEMLMAARRMAIPCVERLGSVLIE
                     DVGVPIPQIPALLAAVAEVADRHDTAIPVIGHAGDGNFHPLVTFDPSDPDAVARADAA
                     FGAVMDAALDLGGTITGEHGVGSLKAAHLATQLGPDVIDLSRRVKAALDPQGILNPGK
                     WL"
     gene            complement(8932..9264)
                     /locus_tag="VLR27_15660"
     CDS             complement(8932..9264)
                     /locus_tag="VLR27_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP04947.1"
                     /translation="MSGTMRGGAGPDAGRPTPEGPLGWTFLTNHSHVLVAVSRDPELR
                     QRDISQQVGITQGAVQRILDDLEAGGYVRRQRVGRRNRYEVIADRPLRHPLEQPRTVA
                     DLLEAVEA"
     gene            complement(9261..9518)
                     /locus_tag="VLR27_15665"
     CDS             complement(9261..9518)
                     /locus_tag="VLR27_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04948.1"
                     /translation="MTPSRALVDELERQVRIIERLWTLAPPARYRDAPRVLGKLEGEI
                     RHLLLRARCGSSDDRIWSEALGCGRRLDRLRAEWHRDPLAA"
     gene            9595..10104
                     /locus_tag="VLR27_15670"
     CDS             9595..10104
                     /locus_tag="VLR27_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HSP04949.1"
                     /translation="MTEPFSPNTDELLAKNAEYAGVFHDSDLQVAPTRNLAVVACMDS
                     RMDIFQILGLGNGEAHIIRNAGGVITDDVIRSLCLSQRFLGTQEIVLLHHTDCGLSKV
                     DEGEFREELADELGVKPWWSLEAFTDPDEDVRQSMHRLQMTPFIPHKDHVRGFVYEVE
                     TGRLREVTA"
     gene            10157..10639
                     /locus_tag="VLR27_15675"
     CDS             10157..10639
                     /locus_tag="VLR27_15675"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HSP04950.1"
                     /translation="MAEVTETQLPGVGVRYEFKTGDKDHVGVVHHHSGRREIVVYDRK
                     DPDVARSVLELSADDSRTLSDLLGASQVTEALAAVQQRIEGLTLEWVEVAEESEMANR
                     SIGDGELRTRTGASVVAVIRGDTSEPAPSPDYVLEPEDVVVAVGTADGLELLRQALRP
                     "
BASE COUNT         1478 a         3823 c         3938 g         1575 t
ORIGIN      
        1 cgccatcgcc accctgggcg ggggcggcgc gctcggcgcc gagggtccct cggtctacgc
       61 cggtgcccgc atcgggccgc gcctcggccg gcggctcacg tcggtgctcg gcccccgggg
      121 tcaccgcgcc ctgacctcgg ccggcgcggc cgccggcgtc gccgccatct tccaggcgcc
      181 cgccaccggc gtgctcttcg ccctcgagac tcccttccag cgcgacgtgg cccggcgcgc
      241 cctcatcccg gccctgatcg ccgcggccac ctcgtacctc accttcgtgt cgatcttcgg
      301 cgtgcactcg tacctgccgg tgccccgggg cgaggtgcgc ctgcgcgacg aggtcttcgg
      361 ggcgatcctg ctcggcctgt gcgccggcat cacggcccgc gggctggccg gtctgtgggg
      421 ggcggccaag ggcctgtccg agcgggtctc ccccgtcgtg cgcctcgcgg ccggcgcggc
      481 cgtcagcggc ctcacgctcg tcgccgcccg ggggctcgtc ggcaccagct tcaccctcgg
      541 cccgggcgtc gagatggtgg ccgagatcgc cgtcgacccc acgatcggcc tctgggtcat
      601 cgtcgccgcc ttcgccctcc gggccctggc gacgtcggca gcgctcggcg cgggaggcgt
      661 cggcggggtg ttcatcccgc tggtggtcca gggtctgctg ctcgggcgcc tcgtggagtc
      721 gctgttcacc gatgccgccc ccggcctcta cccggtcatc ggcctcgccg ccgtcctggg
      781 tgccggctac cgcacgccgc tcgccgccgt gatgttcgtg gccgagacca ccgggcaggc
      841 ccagttcgtg atccccgccc tcgtggccac cgccgtgtcc caggccgtga tgggcgagaa
      901 gagcgtggcg tcgtaccagg tcaccgaacg ggaggggatg ctcgagcggc gcctgcggct
      961 gcccgtgcgc aacgtggtca tcgacgtccc gatcctgcac cccgaccagc tggtcaccga
     1021 cgtgatcgac acctacggcg acgccccgcc ggcccaggcc ctcccggtgt gcgacgaccg
     1081 ctaccggggt ctgctgatgc tgcgcgacat ggccatggcc ctgttcgaga tcggtcccga
     1141 tgccaccgtc ggccaggcca tgcacgacgt gccggcggtg cgggtggacg tccccgccgt
     1201 ggaggcggcg cggatcatgg gcgagctcga cagcgccatc atcgccgtga tcgatgccga
     1261 cgacgtcgcc atcggcgtgg tcaccgccct gacgatgacc ggcgccctcg actacgaccc
     1321 cgacgattag gccgctggcg agggcatagg gtccgggcca tgaagctcgg actctccctc
     1381 ggctactggg gtcgcggccc caacccggac accctcgaca acgtgctcgc cgccgagtcg
     1441 atgggcttcg actcgatgtg gacggcggag gcgtggggtt ccgatgccct caccccgctg
     1501 gcctggtacg ggtcgcgcac gaccacgatg aagctcggtc agagcatcac ccagatcagc
     1561 gcccgcaccc cggccgccac ggcgatggcg atgcagacgc tgcagcacct ctccgacggg
     1621 cgggtcatct gcgggctcgg cgtcagcggt ccgcaggtcg tcgagggttg gtacggccag
     1681 cccttcgcca agccgctggc ccgcacgcgc gagtacgtct cgatcctcaa gcaggtgtgg
     1741 gagcggggcg agccggtcac gagcgagggc ccccactacc cgctccccta ccccgccgac
     1801 gccgagggct ccgtgggcaa gggcaaggcg ctgcgctcca tcacccaccc gcaccccacg
     1861 ccgatcccgg tgtacctggc cgccgagggc cccaagaacc tggccctcgc cggcgagatg
     1921 gccgacggct ggctgccgct gtggatgagc ccggacgagt cgtcctggta cgaggcccag
     1981 atccagaagg gcatggaccg cgtccccggc aagagctggg acgacttcgg cgtcgccgcc
     2041 accgtgccca ccctcgtgct cgacgacctc gacgccgcca tcgacctcat gcgacccaac
     2101 ctcgccctgt acatcggcgg catgggagcc aagggcgaga actaccacta cgacgtgttc
     2161 gcccggatga acgagggtcg ctacgagggg acctgcgagc agatccagga gctgtacctg
     2221 tccggacaca aggacgaggc catcgccacg gtgcctgccg agatggtcga cgacatctgc
     2281 ctggtcggct ccgccgaccg cgtgcgggac cgcgtgcagc gctgggagga ctccatcgtg
     2341 gacaccctcc tcatcggcgg tccctggcag accgtccagg ccgccggcgc cgccctcctg
     2401 gactagctcc tccgctcgag gcggacgatg atcgacttcg aggtcggcgt gttggaggtg
     2461 gtggccaccg agtccaccgg caccagcacg ttggcctcgg ggaagtaggc cgccgccgtc
     2521 cgccgggcga tcgggtactc gacgacccgg aacgacgggg cccgccgatc gtcgtcgccg
     2581 ggccactcgc tgaccaggtc gaccacgtcg ccgtcggtga ggccgaggtc cacgaggtcc
     2641 tgggcgttgc agaacacgac ccgtcggccg ccggtgatcc cccggtagcg gtcgtcgagc
     2701 ccgtagatcg tggtgttgaa ctggtcgtgc gagcggacgg tctgcagcag cagccggccc
     2761 tcggggatcc ggatcggctc gaactcgttg acggtcagct gggcccggcc ggacggggtc
     2821 tcgaaggagc gctcgtcgcg cggtgcgttg ggcagggcga agcgggcccc gccggcgatg
     2881 cgctcctcga agcgctcgaa gcccgggacc acccggctga tccgggtccg gatgcgggcg
     2941 tagtcgtcgg cgaaggatga ccacggcacg gtggaatcgg gcagcgtggc ctcggccagg
     3001 gcgcacacga tggccacctc gctgcggagg tggtccgaca ccggctccac cgagccctgg
     3061 gtggcgtgga cctcgcccat cgagtcctcc accgtcaccg actgcacgag accgccggtg
     3121 cgatcgatct cggtgcgtcc gaggcacggc aggatcagcg cctcctcccc gcacagggcg
     3181 tggctcttgt tgagcttggt cgacacctgc acggtgaggc cgcacgacgc caggccggct
     3241 gcggtcttct cggtgtcggg ggcagcggac acgaagttgc cgcccatccc gacgaacacg
     3301 tcgacgtcgc cgcgtgccat ggcgcggatg gcgtgaacgg tgtcgaggcc gtgctcgcgg
     3361 ggcgggtcga agccgaactc ggcggccagc gcatcgagga acggggcggg cggcttctcc
     3421 cagatgccca tggtgcggtc gccctgcacg ttgctgtgcc cgcgcacggg gcagagcccg
     3481 gcacccggca gcccgatggc gccccgcagc aggtgggtgt tcacgatctc gcggatcgtc
     3541 ggcaccgacc ccttgtgctg ggtgaggccc atggcccagc aggtgatgaa ccgctgggtg
     3601 ccggcgagga ggtcgacgag caggtcgatg tcggcctggg tcagcccggt ggcctcgagc
     3661 cgggcggcgt ggtcgatgcc ggcgagcgac gacgccagcg cgtcgaggcc gtcgacccgg
     3721 tcgtcgacga acccgcggtc gagcgcgccc tgggcgacga gcgcagcgtt gacgagctgg
     3781 aacagggcga ggtcgccccc gacccggatc gccaggtggt ggtcggcgag accggtgccc
     3841 gacccgagca ggccgcgcgc ccgctgcggg ttgcggaagc cgaggaggcc ggcttcgggc
     3901 atggggttga tcgccacgat ggtggcgccc cgctccttgg cccgctcgag cgacgacagc
     3961 atccgggggt ggttggtgcc cgggttctgg ccgacgatga ggatcacgtc ggcgtcgtcg
     4021 aagtcccgga ggctgaccgt ccccttgccg atgccgatcg tcggggtcag cgcgctgccc
     4081 gaggactcgt ggcacatgtt ggagcagtcg ggcaggttgt tcgtgccgag gtcccggacg
     4141 aagagctggt agaggaacgc ggcctcgttg ctcgtccggc ccgatgtgta gaacaccgcc
     4201 cgatccgggc tggtcgcctg caggtggcgg gcgatgacgg cgaaggccgc gtcccagctg
     4261 atcggctggt agtgcgtggc ccccggtggg cggtggaccg ggtcggtgat ccggccctgc
     4321 tggcccagcc accagtcgct gcgctcggcc agctcggcga tggggtggcg ctcgaagaag
     4381 gtggggtcga cgcggcggcg ggtcgcctcc tcggcgacgg ccttcatccc gttctcgcag
     4441 aactcggcgt gggacgggtc ctcggcctcg ggccaggcgc aaccggggca gtcgacgccg
     4501 ccgggctggt tgaggtcgcg caggatccgc gcgccccggc ggatgcccac ccggcgcagc
     4561 acgtggccca gtccgtggcg gacgccgggg aggccggcgg cggtcgtctc cggttccccg
     4621 acgctgacgc caacggcgtc gtcctgggga ccaggatcgt gctcgggggg cggcgacacc
     4681 atgccaggca tggtgtcaga ccctcagcgc atgcgggagg cgcccttgac gatcaccttc
     4741 tggaccacgg ccgccgggag gcgggagacg aagtcgatgg ccttggcgtc gggaccgatc
     4801 agggcccggc ggcggttccg ctgcacggcc ttgatgatct ggcgggctgc cttctcgggc
     4861 gtggtcatga acatgcgctc gaagtcccgg cgggcctgct cggggtcgtc ggccatcgcg
     4921 gtgacgctgt cgtccatgcg ggcgttcttg gcgatgttgg tcttgatccc gccggggtgg
     4981 acggtggtgg cgctgacgcc gcacggctcg atctcgagct ccatgcgcag ggcgtcggtg
     5041 aacccgcgca ccgcgaactt ggccgcgttg tacgccgact gcgacgggat gccgagcagg
     5101 ccgaagacgc tcgagatgtt caccacgtgg ccggcgccgg agtccttcag gtggggcagg
     5161 aacgccttgg tgccgtgcac cacaccccag aagttgatgt ccatcagcca gtggaggtcc
     5221 tcgtagcgag cgccctccac ggtggcgccg agggccaccc cggcgttgtt gaacaccagg
     5281 ttgacctggc cgtggtcgcc gacgacctcg tcggcccagc gctcgacggc gccacggtcg
     5341 gccacgtcga cgcgggccga ggtgaccttg accccgaagc cctcggcctt ggagaccgtc
     5401 tcggcgaggc cgacctcgtc cacgtcgctc agggcgaggt gggtgccggc ccgggccagc
     5461 tcgaccgcga gggcacgccc gatgcccgat cctgcgccgg tgatggcggc cacccggcca
     5521 cggagctgct ccacgtcccg ctccctcacg ccgctcgcgt cgcgagcttg tcgttcaggg
     5581 tcccctcgtc gccgaacagc tcggcgcgcc agcggctgac cagctcgtcg gtgtcgcggt
     5641 cgtcggggtg gaagtccggg tcgtcgtact ccttcagcgt gcgccacgtc tcccgggaga
     5701 ggaacggcga cgccttgagc ttgcggaagc tgcgccacag cttcaccggg tggcgacggg
     5761 cgtcggggtc cagcagcacc gacacgatga cctggaggcc catgccgatg acgaagccgg
     5821 accggatgaa cttcatggtc cacacccgca tgcgctcgga gccacccacg gccttgaaca
     5881 cgtcgaaggc gacggccttg tgctcggact cctcgagcgc gtgccagagg aagaggttct
     5941 tgacctcctc gtggccgatc atgtcccggg cccgctcgtc gctgaggatg atctcggcga
     6001 gggtcgcggt gaagtgctcg agcgcggcgg tggaggccag gttcgagatc ggcggcgcga
     6061 tccgctcccg gagcgccagg cccttcttgg tgaagcgctc ggcggccttg gtcgggtagc
     6121 cgagctggtc gaggcggtcg ttgaactccc ggtgctcccg accgtgcacg gcctcctgac
     6181 cgatgaagcc cgacacctgg cgcttgaggt cggggtcggt gatctccgag cggtagtggc
     6241 gcaccgagcg gacgaagaag tcctcaccgt cggggaacac cgaggagagc gaggcgatca
     6301 ggtggctgcc gatgatgtcg ccgtcctcgg cgaagtgctt gggcaggtcg cgcagcgcct
     6361 cctcgaagga gatgcgccgg gtcggcacgt ctcgcgacgc gttcgacgtc tgggtggtcg
     6421 gcttcggttc ggtgatggtc atcgggggtc ctcctggggg acgagcaggt accgctcgaa
     6481 ggcggcgatg atcacggcgg tgagacggtc gtcggagctg acgaagccgt cgagggcacg
     6541 tcggcggttg aggccgtcga tgacgaggtc gatgaactcc acgacggcct ccgggtcgat
     6601 gtcggggcgc acgaccccgg cgtcgacgga ctcgtggagc agcgggacga aggcgtcgac
     6661 gatgcggtgg ttgatgcggc gctgggcggc aagggcgtcc tcggtgcccg agccggtgcg
     6721 gaacgacgcc aggaccttga ggtcgagcac ccgtacgagc tggtcgacca gggcccggac
     6781 gacgccctcc atgggggtgc cgagctcgag ctcggcgtgc acgagcgtgg tgagctcatc
     6841 ggtcttgtgg tcgaccacgg cggccagcag ggcctccttg gagtcgaagt gcccgtagaa
     6901 catcccggcg gagacgccgg cctcggtgac gatggcccgg gtggtgaggc cgtccacgcc
     6961 gtcgcgctcg gcgaccgcga cggtggcgtc gatcagccgc tggcgggcgg cggctcgctg
     7021 ctcctcgctg atcctgggca tggcggtcct tccgaaaccg agcgctcgtt ctgtttccgt
     7081 tgttcgccgt gccgatgcgc gatcccttcc gcagtggcaa gagccacacc atccggtacc
     7141 cttccgcacg tcacttcgtg ggcggagatg acccttgagg agtcctccgt gcccgcaccc
     7201 gaccagcaca cgagcccgtt ctccgaaccg ggcgacctgt tcgcgtccgt cgccttcatg
     7261 gcgctcggcg cgtgccgggg cgccgatccc gacctgttct tccccgaccg gggcgagtcc
     7321 ctcgagccgg ccaagcgcat ctgcagcgag tgcgtcgtgc gcgatgagtg cctcgagcac
     7381 gccctcgcca gcggcgagcg cttcggcgtg tggggcggga cgtcggagcg cgagcgccgc
     7441 cgactgcgcc gcacccgtcg caccgccgcc gcctgaccgc cgcgctacct cacgactgcc
     7501 agttccagga gattgaaact ggcagttccc ctgtcgcgcg ctacagccac ttcccggggt
     7561 tgaggatgcc ctgggggtcg agggccgcct tcacccggcg gctcaggtcg atcacgtcgg
     7621 gcccgagctg ggtggccagg tgcgcagcct tcagggagcc cacgccgtgc tcgccggtga
     7681 tcgtgccgcc gaggtcgagg gcggcgtcca tcacggcgcc gaaggcggca tcggcccggg
     7741 cgacggcgtc ggggtccgag ggatcgaagg tgacgagcgg gtggaagttg ccgtcgccgg
     7801 cgtggccgat gacggggatg gcggtgtcgt ggcgatcggc gacctcggcc acggcggcga
     7861 gcagcgccgg gatctgcggg atgggcacgc ccacgtcctc gatcagcacg ctgccgaggc
     7921 gctccacgca cgggatggcc atgcgccggg cggccatcag catctcgccc tcgtccgggt
     7981 cgtcggtgac ggccacgtag gtggcgcccg cggcctcggc gtgctcggcg atgcgggcgg
     8041 cggtcgcgtc cccggcgtcg gactggccga gcagcagggc accgatcgag gtgtcgagcc
     8101 ccatcggctt caccgactcc accgcggcca cggctgcctg gtccatgagc tcgagggccg
     8161 aggggcgcag cgacgccatc gttgccgcca cggcgacgcc ggccgcgacc gggtcggcga
     8221 acgtggccac gacggtggac gacgggggcg gggtgggtcg caggcgcagc gtggcctcgg
     8281 tgatgacgcc gagggtgccc tcggatccga cgaagagccg cttgaggtcg agcccggcga
     8341 cgtccttgat ggtccgcccg ccgagccggg tgacggtgcc gtcggccagc accacctcga
     8401 tgccgagcac gtagtcggtg gtcacgccgt acttcacgca gcacaggccg ccggcattgg
     8461 tggccaggtt cccgccgatg gagcagatct cgtaggacga cgggtccggc gggtaccaga
     8521 gcccctcggc ccgggccgca gccttgacct cgacgttgag cgcgcccggc tgggccacgg
     8581 cgaccatcga cccggcgtcg atgcgcacct cgcgcatgcg ctcggtcgac agggtgatgc
     8641 agccgtccac tgcagccgac ccgcccgaca ggccggtccc tgcaccgcgg gtcaccactc
     8701 ccacgccgaa ccggctcgcc acgcgcatgg tggcagccac ctcgtcggtc gagccggccc
     8761 ggaccagcgc gatcggcgta ccgggcacca ccgtcttggc ccggtcgaag cggtagccgt
     8821 ccaccacgtc ggggtcgacg accaccgact cggccgggag cgcatcttgc agcgcggcga
     8881 cgacgtcggc gggacccatc gccgtcgagt ctgccctgcg ccggcggtcc gtcaggcctc
     8941 gacggcctcc aggaggtcgg cgaccgtccg cggctgctcg agcgggtggc gcagcgggcg
     9001 atcggcgatg acctcgtagc ggttgcgccg gccgacgcgc tggcggcgga cgtagccgcc
     9061 ggcctcgagg tcgtcgagga tgcgctgcac ggcgccctgc gtgatgccga cctgctggct
     9121 gatgtcccgc tggcgcagct cggggtcgcg actgacggcg acgaggacgt ggctgtggtt
     9181 ggtgaggaac gtccagccca ggggaccctc tggcgtgggc cggcccgcat cgggaccggc
     9241 cccaccgcgc atcgtgccgc tcatgcggcc agggggtcgc ggtgccattc ggcccgcagc
     9301 cggtcgagcc ggcgtccgca gccgagcgcc tcggaccaga tccgatcgtc gctgctgccg
     9361 catcgggctc gcagcagcag gtgccggatc tcgccctcca gcttcccgag cacccgagga
     9421 gcatcgcggt agcgagccgg cggggcgagg gtccagagcc gctcgatgat ccgtacctgg
     9481 cgctcgagct cgtcgacgag tgcacgcgat ggggtcatgg gacgagccaa acatacccat
     9541 tgcaataggt caatcagatt gcgtcagttg ccatcgagcc gtaacctctc gcccatgacc
     9601 gagcccttct cccccaacac cgacgagctc ctggccaaga acgccgagta cgccggcgtc
     9661 ttccacgaca gcgacctcca ggtcgccccc acccggaacc tcgccgtggt ggcctgcatg
     9721 gactcccgca tggacatctt ccagatcctc gggctgggca acggcgaagc gcacatcatc
     9781 cgcaacgccg gcggggtcat caccgacgac gtcatccgct ccctctgcct gtcccagcgc
     9841 ttcctcggca cccaggagat cgtgctcctg caccacaccg actgcggcct ctccaaggtc
     9901 gacgagggcg agttccgcga ggagctcgcc gacgagctcg gggtgaagcc gtggtggtcg
     9961 ctcgaggcgt tcacggaccc cgacgaggac gtccgccagt cgatgcaccg cctccagatg
    10021 accccgttca tcccccacaa ggaccacgtg cgcgggttcg tgtacgaggt cgagaccggt
    10081 cgactgcggg aggtcaccgc ctgagcgctc cgcaccatcg ggcagactga cggcgacgcc
    10141 gacgacagga gcgccgatgg ccgaggtgac cgagacgcag ctgcccgggg tgggtgtgcg
    10201 ctacgagttc aagacgggtg acaaggacca cgtcggcgtg gtccaccacc actcgggccg
    10261 tcgcgagatc gtcgtgtacg accgcaagga ccccgacgtg gcccgctccg tgctcgagct
    10321 gagcgccgac gacagccgca cgctcagcga cctgctcggc gccagccagg tcaccgaggc
    10381 actcgccgcg gtccagcagc gcatcgaggg cctcaccctc gaatgggtcg aggtcgccga
    10441 ggagtccgag atggccaacc ggagcatcgg cgacggcgag ctccgcaccc gcaccggcgc
    10501 atcggtcgtg gccgtgatcc gcggcgacac cagcgagccg gcgccgagcc cggactacgt
    10561 gctcgaaccc gaggacgtcg tggtcgccgt cgggaccgca gacggactgg agctcctccg
    10621 gcaggcgctg cgcccgtgac cacggtgctg gccgcggtca gcggggagac ggccgaggcg
    10681 ttcatccaga tcggtggggt ggtcgtcgtg ctggccgtgc tggcgcgcat cgcaggccga
    10741 ctcggcatcc cggccatccc cctcgacctg ctcgccgggc tcgccgtcgg cgagggcggg
    10801 ttcacctcgc tcga
//
LOCUS       DATISU010000395         1918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_220109, whole genome shotgun sequence.
ACCESSION   DATISU010000395 DATISU010000000
VERSION     DATISU010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220109"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VLR27_15680"
     CDS             <1..895
                     /locus_tag="VLR27_15680"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HSP04951.1"
                     /translation="VAVAGSTMHFLQAILLLFVLYSFVGVPGGSLTEDKEWEVGEVVP
                     GSAAADAGLRADDRVIEVDGRRYETFTTFAEDIAERPGEEVTLSVLRDGDLLSVPVEI
                     GIAPSDADRGLLGIRRDLVTTTVGPVRAVGTSISDFWDQTTATLGFIGSFSPTDFWDS
                     VVSSGSEPAAVESRDPAPGGPQPSSSEAPDENRIISIVGAVGIGADLTENGLLGFLLF
                     FVGINITIGIFNLLPVLPLDGGHVVVATYERIRELLRPGKARYHVDMAKLLPVVYVVF
                     FFLVLLGVTTIYMDITDPVRL"
     gene            895..>1918
                     /gene="ispG"
                     /locus_tag="VLR27_15685"
                     /gene_synonym="gcpE"
     CDS             895..>1918
                     /gene="ispG"
                     /locus_tag="VLR27_15685"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HSP04952.1"
                     /translation="MQIGESSFPRRTTRQIQVGDVAVGGGAPISVQSMTVTKTADVDA
                     TLQQIYALAAAGCDIVRCTCNEPEAAEGLARIIPRSPVPIVADIHHQYKRALEALEAG
                     VHCLRLNPGNIRKPEHIKAVAMEAKDRGVPIRIGVNGGSLDPALYEKYGGKVTPEAMV
                     ESAKIEIGYFEEVGFEDIKISVKASSVPLMIEAYRQLADAVDYPLHLGVTEAGPPPGG
                     LIKATAGIATLLAEGIGDTIRYSLTADPVQEAKAGRQLLEALGLRERKTVDQIAGPSC
                     GRAEVDVIGVAIRAMEAFAGREIPLQIAVMGCVVNGPGEARDADLGIAAGNKRGHLFV
                     KGQNVAV"
BASE COUNT          289 a          729 c          617 g          283 t
ORIGIN      
        1 cgtggccgtg gccggctcca ccatgcactt cctgcaggcc atcctcctgc tgttcgtcct
       61 gtactccttc gtcggcgtcc ccggcggcag cctcaccgag gacaaggagt gggaggtcgg
      121 cgaggtcgtg cccggcagcg ccgccgccga cgccggcctg cgcgccgacg accgcgtcat
      181 cgaggtcgac ggtcgccgct acgagacgtt caccaccttc gctgaggaca tcgccgagcg
      241 acccggcgag gaggtcaccc tgtcggtgct ccgcgatggc gacctgctca gcgtgcccgt
      301 cgagatcggc atcgccccga gcgacgccga ccggggcctg ctcggcatcc ggcgggacct
      361 cgtcacgacc acggtcggcc cggtccgcgc cgtcggcacc tcgatcagcg acttctggga
      421 ccagaccaca gccaccctcg gcttcatcgg ctcgttcagc ccgaccgact tctgggacag
      481 cgtcgtgagc agcggcagcg agcccgccgc cgtcgagtcc cgtgatcctg cccccggtgg
      541 tccgcagccc tccagctccg aggcccccga cgagaaccgc atcatctcga tcgtgggtgc
      601 ggtgggcatc ggtgccgacc tcaccgagaa cggcctgctc ggcttcctgc tgttcttcgt
      661 cggcatcaac atcaccatcg gcatcttcaa cctgctgccg gtgctgcccc tcgacggcgg
      721 ccacgtggtg gtcgccacct acgagcgcat ccgcgagctg ctccggcccg ggaaggcccg
      781 ctaccacgtc gacatggcga agctgctgcc ggtggtctac gtcgtgttct tcttcctggt
      841 gctgctcggc gtcaccacca tctacatgga catcaccgac ccggtccggc tctgatgcag
      901 atcggcgaat ccagcttccc ccgtcgcacc acccgccaga tccaggtcgg cgacgtcgcc
      961 gtcggcggcg gtgcgcccat cagcgtccag tcgatgacgg tgaccaagac cgccgacgtc
     1021 gacgccaccc tccagcagat ctacgcgctc gccgccgcag gctgcgacat cgtgcgctgc
     1081 acctgcaacg agcccgaggc ggccgagggc ctggcccgca tcatcccccg ctcgccggtg
     1141 ccgatcgtcg ccgacatcca ccaccagtac aagcgtgccc tcgaggcgct cgaggccggc
     1201 gtgcactgcc tgcgcctcaa cccgggcaac atccgcaagc ccgagcacat caaggccgtg
     1261 gccatggagg ccaaggaccg gggcgtgccc atccgcatcg gcgtcaacgg cggcagcctc
     1321 gaccccgccc tgtacgagaa gtacggcggc aaggtcaccc ccgaagccat ggtcgagtcg
     1381 gccaagatcg agatcgggta cttcgaggag gtcggcttcg aggacatcaa gatctcggtg
     1441 aaggcgtcgt ccgtgccgct gatgatcgag gcctaccgcc agctcgccga cgccgtcgac
     1501 tacccgctgc acctcggcgt caccgaggcc ggcccgcccc ccggcggcct catcaaggcc
     1561 accgccggca tcgccaccct gctggccgag ggcatcggcg acacgatccg ctactcgctc
     1621 accgccgacc cggtccagga ggccaaggcc ggccgccagc tgctcgaggc cctcggtctg
     1681 cgggagcgca agaccgtcga ccagatcgcc ggcccgtcgt gtggccgcgc cgaggtcgac
     1741 gtgatcggcg tggcgatccg ggcgatggaa gcgttcgccg gtcgcgagat ccccctgcag
     1801 atcgccgtga tggggtgcgt ggtcaacggc ccgggtgagg cgcgcgacgc cgacctcggc
     1861 atcgctgcgg gcaacaagcg cggtcacctg ttcgtgaagg gccagaacgt ggccgtgg
//
LOCUS       DATISU010000396        10900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_778370, whole genome shotgun sequence.
ACCESSION   DATISU010000396 DATISU010000000
VERSION     DATISU010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10900
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778370"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /gene="rpsI"
                     /locus_tag="VLR27_15690"
     CDS             <1..382
                     /gene="rpsI"
                     /locus_tag="VLR27_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HSP04953.1"
                     /translation="LVQATGRRKRAVARIRLRPGTGTVTVNGKDVKVYFPTDALQSLA
                     MAPIAVAEAEEHYDIDCTIVGGGTTGQAGAMSLGLARAFAELDPERRGELKHEGLLRR
                     DPRKKESKKYGLKKARKAPQYSKR"
     gene            396..1733
                     /gene="glmM"
                     /locus_tag="VLR27_15695"
     CDS             396..1733
                     /gene="glmM"
                     /locus_tag="VLR27_15695"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HSP04954.1"
                     /translation="MLSFGTDGVRGVANQDLTPELALALGRASARVLGGDAVVIGRDT
                     RRSGPMLEAALAAGYASEGVAVTLLGVVPTPTVAHAAAARGVPGAMISASHNPFPDNG
                     IKLFAAGGTKLADEDEDQLEAELAHLLGSGGGDRPTGAGVGSVAADDGTLARAYADDL
                     HRAIGGRNLAGLSVVLDCAHGAASAIAPEVLRAAGVEVTVVHAEPDGVNINADAGSTS
                     PEALQRAVVDHGADVGLALDGDADRLLAIDAGGTLVDGDHVIAILAIDRQAQGRLPDD
                     TVVATVMSNLGFRQGMARRGITVVDTKVGDRYVLEALARTGAALGGEQSGHVIQADLA
                     TTGDGLLTALTLLDVVHRTGRPLGDLAADAMTSLPQVLVNVRLERRDPDLVERLAGDV
                     VEAEARLGDDGRVLVRPSGTEPLIRVMVEAPTAEQAQQEADTLAAAVRRLAGA"
     gene            1779..2612
                     /locus_tag="VLR27_15700"
     CDS             1779..2612
                     /locus_tag="VLR27_15700"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HSP04955.1"
                     /translation="MRYALGLLALFVAAAVLNACGDDDESVDTRGGPAALAAEYTSVE
                     VTEAGAPRPLVAGTAISLRFDEGRVGASLGCNQLGGPYELDGDRLLVEDLSMTEMGCD
                     PERHAQDEWFAGVLQSEPTVVVDGDTMTLTAGETVVRFVDREVAEPDQELVGTTWEVD
                     GFADGQDPEDAAMSVAVDQPATVRFGEDGFVTGNDGCNAFGTGEVDGTQDGLRYQLDG
                     DRVVLSGAPVSTQVACPDVEDYVDRFWAALSGTVTWSVDADRLTLVGEDGRVVTFRAT
                     D"
     gene            2691..6152
                     /locus_tag="VLR27_15705"
     CDS             2691..6152
                     /locus_tag="VLR27_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HSP04956.1"
                     /translation="MCGIIAVLRRPVDRVDLPAEPVVAALDRGVQSLRRAIDDAPEPG
                     PAIEALQEVAVDLEQVADDLHGPRGVRLLLTDTATSARIRGLAKTLGQTVVELDAALG
                     LLVVDDDTEARNAAAVRVKDAAWTIERDRLRLADGVATLVGRDTGWSAIEACTQIEQA
                     LSAIDRLEVRGRDSAGVMVLVRDHGLDPTDPAVIGLLDGRADDPLLRSRAVRLVDGPA
                     GPASQLAFAYKAAAEIGELGDNTAAIRAHIVGDELLHLALASPDAQAVVLGHTRWASV
                     GIISEANAHPLDSLEQEQAEVGPFVAAALNGDVDNFADLKSANGLSIHAEVTTDAKVI
                     PTLVSRRVVAGDDALEAFRASVASFEGSVAIAATVAGRPDALALALRGSGQALYVGLV
                     DDAYIVASEPYGLVEETSRYVRMDGETPANPDNPNASRGQILLLDGAKAGSLEGIERI
                     AYDGTVLPVTEDDVVTATITTRDIDRGDFPHFLLKEITESPVSFHKTLRGRLIEDGDE
                     LRVVLPEDSFPADIRARLADGSITRILAIGQGTASIASRGFAALLADAADRRGVQLEA
                     IPATELSGFRLRSDMSDTLIVAISQSGTTTDTNRTVDLVRGRGASVIAIVNRRGSDLT
                     DRAEGVLYTSDGRDVEMAVPSTKAFYAQIAAGNLLAIAIAAEWGEEPDQSLLRAMRDL
                     PDAMRATLATRPAIAEAAHEWCPPRRYWALVGNGTNRIAAEELRIKLSELCYKSIACD
                     ATEDKKHIDLSSEPLILVCAAGLTGSNVDDVAKEVAIYRAHKAAPIVIATEGEARFAA
                     ALAVLTVPETHEALAFILATMVGHLFGYEAALAIDAQATPLKEVRGGIEAAIEGGALD
                     GERVLSELRTSVMPPVQTFFDGLRAGRYDGHLEASTAVRTANLLRYASGAVALDAYQL
                     DHGKVGTPSVLVEDLTAALTRAIDELTRPIDAIKHQAKTVTVGISRTDESMLEVPLVR
                     EVLAAGAPRDRLTYKNLRTLADVSPAVEEVVGYTRYRIEGTVDDTSGAGATITVVDRG
                     GIARDLPLRTEQNPVLRGTKHQVAADQELLVGRGRSDDRTIVIVPEVKDGTTTGITLL
                     HVRFADRLSVASARGVLSGYRRRLAALRDAVTETEPTFRDDLLAEVPVVDLLTLPVSL
                     LADRWRS"
     gene            6169..6552
                     /locus_tag="VLR27_15710"
     CDS             6169..6552
                     /locus_tag="VLR27_15710"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HSP04957.1"
                     /translation="MSPVGVVGVGIDLVDVDRFRVSLARTPSMRERMFTDGERAYAET
                     ANDPTERYAARFAAKEAVMKAMGVGLGAFGFHDVEVLRADGGQPSLRVIGAAELLAVD
                     LGVRGWQLSLTHTATSAGAVVVALG"
     gene            complement(6616..7518)
                     /locus_tag="VLR27_15715"
     CDS             complement(6616..7518)
                     /locus_tag="VLR27_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04958.1"
                     /translation="MSDLQTTHRTTVTEEMIDHLGHMNVRYYGVAARAASDAVVRDLG
                     GAGDLSVQAVDIYTRHFREQLVDARLEVRSTVLSHGGGVLRLYHELRNTDTDDLAASF
                     VHRLEARHGDDLVGTWPPAGDLRTGDIPEHGRPRSIALDTAPLETAPTLADVQADELL
                     AMRRPRTIEADECRPDGAYDPAAAPFLLWGGEPLEGATGPTLHPGPDGQLVGWATMEN
                     RMVIHRLPRRGDRIQSFGAVVEIRDKTTHRVMWGYDLDRGDLLVAFEVVDVALDTVSR
                     RAVPIPAELRRHAEDRFRPELLPR"
     gene            7609..8973
                     /locus_tag="VLR27_15720"
     CDS             7609..8973
                     /locus_tag="VLR27_15720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HSP04959.1"
                     /translation="MLPVVRIAEMQAIDADAPEPVEVLIDRAGAAVARAALDLLGGGY
                     GRRVVVLAGPGNNGADGRVAAEKLRRRGLRVDVIELADAPDVLPAADLVIDAALGTGA
                     SRPHHAPDPGAALVLAVDIPSGIHGDTGEAVGSPMRADLTITFAALKPGLLLGDGPAH
                     TGAIRIADIGLDVSRARMHLLDDAGAAALLPRRDTAAHKWHRSVLVVAGSPGMTGAAS
                     MAAEAAQRAGAGMVRLATPGGGHGADVPREVVTLDVEEAGWAEALVDDPGKVHALAVG
                     PGMGTAMATRHQIRTLVAGIEAPVVVDGDGLTALGTDAASVIGRRRAGTVLTPHDGEF
                     ERLAGHAPGTDRVADVVALAERTGAVVLLKGPTTVVAAPDGQIRLARAGDDRLATAGT
                     GDVLTGIVAALLAGGLDPVDAAAVGAHVHGLAGRAGPRHGTMASDVIDGIPAALGHLL
                     GDER"
     gene            8970..10127
                     /gene="alr"
                     /locus_tag="VLR27_15725"
     CDS             8970..10127
                     /gene="alr"
                     /locus_tag="VLR27_15725"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HSP04960.1"
                     /translation="MTADPGYGGSVAEIDLDAIAHNVAVLDDHAGDAQVCAVVKADGY
                     GHGAVPVARAALAAGATWLGVAQVQEARALRAAGIDAPVLVLSECDTSADTLDHALAM
                     GLHLVGYRPGFLDAVSERARVLGTRPAPVHLKVDTGMRRVGCEPHQAVDLARRIVEDP
                     HLVLAGTMTHLAVADEPGNLGTAVQLDRFEAVLAGFDEAGIDPGVRHAANSAATLLHP
                     RAHLDLVRTGIAVYGIPPSPVLSGVAALRPAMRWSSSVRFVKPVARGEGVSYGHRHVF
                     EEATVVATVPVGYADGYRRRLGLVGGVVLIRGRRCPIVGVVTMDQLVVDVGPDGDVEV
                     GDEVVLVGAQGDDEVTATELALLVDTIGYEITCAVSSRVARHHHGGPEDSA"
     gene            10127..>10900
                     /locus_tag="VLR27_15730"
     CDS             10127..>10900
                     /locus_tag="VLR27_15730"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HSP04961.1"
                     /translation="MVTDVDGVRVGHWTDAEARTGCTVVLLPEGTVASGEVRGGAPAT
                     REFALLDPTRMVVRMDAVVLSGGSAFGLAAADGVMAHLAEAGVGFETVGGPVPIVVGM
                     ALYDLAVGDPSVRPTAEHGRAAAEAATGGGVDTGLVGAGTGATVAKWRGPDHRRPGGL
                     VAATRRGRDGLVVAARVAVNAFGEPGADPGDVADGSVGGSDFTEGAPGTNTTIGVVVT
                     NARLDKVACHLLAQSAHHGLARSVSPSHTSVDGDAFVAAA"
BASE COUNT         1397 a         4222 c         3883 g         1398 t
ORIGIN      
        1 cctcgtccag gcaaccggcc gtcgcaagcg cgccgtcgcc cgcatccggc tccgtcccgg
       61 caccggcacc gtcaccgtga acggcaagga cgtgaaggtc tacttcccga ccgacgccct
      121 ccagagcctg gccatggccc cgatcgccgt cgccgaggcc gaggagcact acgacatcga
      181 ctgcaccatc gtcggtggtg gcaccaccgg ccaggccggc gccatgagcc tcggtctcgc
      241 ccgtgcgttc gccgagctcg accccgagcg ccgtggcgag ctgaagcacg aaggcctcct
      301 gcgccgcgat ccgcgcaaga aggagtccaa gaagtacggc ctcaagaagg cccgcaaggc
      361 gccgcagtac tccaagcgct gatccgcccc ggcggatgct gtccttcggc accgacggcg
      421 tccgcggcgt cgccaaccaa gacctgacac cggagctcgc cctcgcactg gggcgagctt
      481 cggcgcgcgt gctgggcggt gatgccgtgg tcatcggccg cgacacccgg cgctccggcc
      541 ccatgctcga ggcggcgctc gccgccggct acgcctccga gggcgtggcg gtgaccctgc
      601 tcggcgtggt gcccacgccc acggtcgccc acgccgcggc ggcacgaggt gtccccggcg
      661 ccatgatctc ggcgtcgcac aacccgttcc ccgacaacgg catcaagctg ttcgcggccg
      721 gcggcaccaa gctggccgac gaggacgagg accagctgga ggccgagctc gcccacctgc
      781 tcggcagcgg cggtggcgac cgccccaccg gcgcgggcgt cgggtcggtg gcggccgacg
      841 acggcaccct ggcccgggcc tacgccgacg acctccaccg ggccatcggg ggccggaacc
      901 tcgccgggct ctcggtggtg ctcgactgcg cccacggtgc tgcgtcggcc atcgcccccg
      961 aggtcctgcg cgctgccggc gtcgaggtca ccgtggtcca cgccgagccc gacggcgtga
     1021 acatcaacgc cgatgccggg tccacgtcgc ccgaggccct gcagcgggcg gtggtcgacc
     1081 acggggccga cgtcggcctc gccctcgacg gtgatgccga ccggctgctc gccatcgacg
     1141 ccggcggcac cctcgtcgac ggcgaccacg tgatcgccat cctcgccatc gaccgccagg
     1201 cccagggccg cctgcccgac gacaccgtcg tggccacggt catgtcgaac ctcggcttcc
     1261 gccagggcat ggcccgccgg ggcatcacgg tcgtcgacac caaggtgggg gaccgctacg
     1321 tcctcgaggc gctggcccgc acgggcgcag cgctcggtgg cgagcagtcc ggccacgtga
     1381 tccaggctga cctggccacc accggcgacg ggctcctcac cgccctgacc ctgctcgacg
     1441 tggtccaccg caccggccgt ccgctcggcg acctggcggc tgacgccatg acgtcgctgc
     1501 cccaggtgct ggtcaacgtg cgcctcgagc gccgcgaccc ggacctcgtg gagcggctcg
     1561 ccggcgacgt cgtcgaagcc gaagcacgcc tcggcgacga cgggcgcgtg ctcgtgcgcc
     1621 cgtcgggcac cgagcccctc atccgggtca tggtcgaagc ccccacggcc gagcaggccc
     1681 agcaggaggc cgacaccctg gccgccgccg tccgtcgcct cgccggcgcc tgaccgcccc
     1741 tccgggaact ggcagtcgct gcgtagcgtc gaaggggcat gaggtacgcg ctcggactgc
     1801 tcgccctgtt cgtggccgcg gcggtgctca acgcctgcgg tgacgacgac gagtccgtgg
     1861 acacccgtgg tggcccggcg gccctggcgg ctgagtacac ctcggtcgag gtgaccgagg
     1921 ccggagcgcc gcgaccgctc gtggcgggaa ccgccatctc cctgcgcttc gacgagggcc
     1981 gggtgggggc ctcgctcggc tgcaaccagc tgggcggtcc ctacgagctg gatggcgatc
     2041 gcctgctggt cgaggacctg agcatgaccg agatgggttg cgaccccgag cgccacgccc
     2101 aggacgagtg gttcgccggg gtcctgcagt ccgagcccac ggtcgtcgtg gacggagaca
     2161 ccatgacgct gacggctggg gagacggttg tgcgcttcgt cgaccgcgag gtggccgagc
     2221 ccgatcagga gctggtcggc accacgtggg aggtcgacgg gttcgccgac ggccaggatc
     2281 ccgaagacgc cgccatgtcc gtcgcagtcg atcagccggc gacggtgcgc ttcggggagg
     2341 acggcttcgt gaccggcaac gacgggtgca acgcgttcgg gaccggtgag gtcgacggca
     2401 cgcaggacgg ccttcgctac cagctcgacg gcgatcgggt cgtcctgtcc ggggcgcccg
     2461 tgagcacgca ggtcgcgtgt ccggacgtgg aggactacgt cgaccggttc tgggcggcgc
     2521 tctccggcac ggtcacctgg tccgtcgacg ccgaccggct caccctggtc ggcgaggacg
     2581 gccgcgtcgt gaccttccgc gccaccgact gaggcgcgac aggagcactg ccagttccag
     2641 aagaggtcgt ccggtgggct ccggggccgg accgggcaga atggtcggcc atgtgcggaa
     2701 tcatcgccgt cctccggcgc cccgtcgacc gggtcgatct cccggccgag ccggtggtcg
     2761 ctgccctcga ccgtggcgtg cagtcgctgc gccgcgccat cgacgacgcg cccgagcccg
     2821 ggccggcgat cgaggcgctg caggaggtcg ccgtcgacct cgagcaggtc gccgatgacc
     2881 tgcacggtcc ccgtggcgtc cgcctgctgc tcaccgacac ggcgacgtcc gcccgcatcc
     2941 gtggtctggc caagaccctc ggccagaccg tggtcgagct cgacgccgcc ctcggcctgc
     3001 tggtggtcga cgacgacacc gaggcccgca acgccgccgc cgtccgggtg aaggacgcgg
     3061 cctggaccat cgagcgcgac cgcctgcgcc tcgccgacgg cgtggccacc ctggtcggcc
     3121 gcgacaccgg ctggtcggcg atcgaggcgt gcacccagat cgagcaggcg ctgtcggcca
     3181 tcgaccgcct cgaggtccgg ggccgggact cggccggggt catggtgctc gtccgcgacc
     3241 acgggctcga cccgacggat ccggccgtca tcggcctgct ggacgggcgc gccgacgatc
     3301 ccctgctgcg ctcgcgcgcc gtgcgcttgg tcgacggccc cgccggtccg gccagccagc
     3361 tggccttcgc ctacaaggcc gccgccgaga tcggcgagct cggcgacaac accgccgcca
     3421 tccgcgccca catcgtcggc gacgagctgc tgcacctggc cctcgcgtcg cccgacgccc
     3481 aagcggtcgt cctcggtcac acgcgctggg ccagcgtcgg catcatctcc gaggccaacg
     3541 cccacccgct ggactccctg gagcaggagc aggccgaggt cggcccgttc gtggccgcgg
     3601 cgctcaacgg cgacgtcgac aacttcgccg acctgaagtc ggcgaacggc ttgtcgatcc
     3661 acgccgaggt caccaccgac gccaaggtga tccccaccct cgtctcccgt cgggtggtgg
     3721 ccggcgacga cgccctcgag gcgttccgcg ccagcgtggc ctccttcgag ggcagcgtcg
     3781 ccatcgccgc cacggtggcc ggtcgccccg acgccctcgc cctggcgctg cgcggctcgg
     3841 gccaggccct ctacgtcggc ctggtcgacg acgcctacat cgtcgcctcc gagccctacg
     3901 gcctggtcga ggagaccagc cgctacgtgc gcatggacgg cgagaccccg gccaaccccg
     3961 acaaccccaa cgccagccgg ggccagatcc tgctgctcga cggggccaag gccgggagcc
     4021 tcgagggcat cgagcggatc gcctacgacg gcaccgtcct tcccgtcacc gaggacgacg
     4081 tcgtcaccgc caccatcacc acgcgcgaca tcgaccgggg cgacttcccg cacttcctgc
     4141 tgaaggagat caccgagtcg ccggtgagct tccacaagac gctgcggggc cggctgatcg
     4201 aggacggcga cgagctccgg gtcgtgctgc ccgaggactc cttccccgcc gacatccggg
     4261 cccggctcgc cgacggctcc atcacccgca tcctcgccat cgggcagggc acggcgtcga
     4321 tcgccagccg cgggttcgct gcgctcctcg ccgacgccgc cgaccgccgg ggcgtgcagc
     4381 tcgaggccat cccggccacc gagctgtccg gcttccggct ccgcagcgac atgtccgaca
     4441 cgctgatcgt cgccatcagc cagtcgggca cgaccaccga caccaaccgc acggtcgacc
     4501 tcgtccgggg tcggggggcg tcggtgatcg ccatcgtcaa ccggcggggc tccgacctca
     4561 ccgaccgggc cgagggcgtg ctctacacgt ccgatggccg cgacgtggag atggcggtgc
     4621 cgtcgacgaa ggcgttctac gcccagatcg ccgccggcaa cctgctcgcc atcgccatcg
     4681 ctgccgagtg gggcgaggag cccgaccaga gcctgctgcg cgccatgcgc gacctgcccg
     4741 acgccatgcg ggccacgctg gcgacccgtc cggccatcgc cgaggccgcc cacgagtggt
     4801 gcccgccccg gcgctactgg gccctcgtcg gcaacggcac caaccgcatc gccgccgagg
     4861 agctgcggat caagctgtcg gagctctgct acaagtcgat cgcctgcgac gccaccgagg
     4921 acaagaagca catcgacctg tcctccgagc cgctgatcct ggtctgcgcg gccgggctca
     4981 ccgggtccaa cgtggacgac gtggccaagg aggtggcgat ctaccgggcc cacaaggccg
     5041 ctccgatcgt catcgccacc gagggcgagg cccgcttcgc cgccgccctg gccgtgctca
     5101 cggtccccga gacccacgag gcgctggcgt tcatcctcgc caccatggtc ggccacctgt
     5161 tcggctacga ggccgccctg gccatcgacg cgcaggccac cccgctcaag gaggtccgcg
     5221 gtggcatcga ggccgccatc gagggcggcg ccctcgacgg tgagcgcgtc ctgtccgagc
     5281 tgcggacgtc cgtgatgccg cccgtgcaga cgttcttcga cggcctgcgg gccggccgct
     5341 acgacgggca cctcgaggcc tccaccgcgg tgcgcaccgc caacctgctc cgctacgcca
     5401 gtggcgccgt ggccctcgac gcctaccagc tcgaccacgg gaaggtcggc acgccgtcgg
     5461 tcctggtcga ggacctcacc gccgccctca cccgcgccat cgacgagctc acccggccca
     5521 tcgacgccat caagcaccag gccaagaccg tcaccgtggg catctcccgc accgacgagt
     5581 ccatgctcga ggtgcccctc gtgcgcgagg tcctggcggc tggtgcgccc cgcgaccggc
     5641 tcacctacaa gaacctgcgc acgctcgccg acgtgtcgcc ggccgtcgag gaggtcgtgg
     5701 gctacacccg ctaccgcatc gagggcaccg tcgacgacac gtccggcgcc ggcgccacca
     5761 tcaccgtcgt cgaccggggc ggcatcgccc gggacctgcc cctgcgcacc gagcagaacc
     5821 cggtgctgcg cggcaccaag caccaggtcg ccgccgacca ggagctgctg gtcggtcggg
     5881 gccgcagcga cgaccgcacc atcgtgatcg tccccgaggt caaggacggc accaccaccg
     5941 gcatcacgct gctgcacgtc cgcttcgccg accggctctc ggtcgcgtcc gcacgcggcg
     6001 tgctgtccgg ctaccgccgc cggctggccg cgctgcgcga cgcggtcacc gagaccgagc
     6061 cgaccttccg cgacgacctg ctggccgagg tcccggtggt cgacctgctc accctgcccg
     6121 tcagcctcct ggccgaccgg tggcggtcct gagcggggaa cgccggtcgt gagcccggtg
     6181 ggcgtggtcg gcgtgggcat cgacctcgtc gacgtcgacc gcttccgcgt ctcgctggcc
     6241 cgcacgccgt ccatgcgcga gcgcatgttc accgacggtg agcgggccta cgccgagacc
     6301 gccaacgacc ccaccgagcg ctacgcagcc cgcttcgccg ccaaggaggc ggtcatgaag
     6361 gcgatgggcg tgggcctcgg tgccttcggg ttccacgacg tcgaggtgct ccgcgccgac
     6421 gggggccagc cgtcgctgcg cgtcatcggt gccgccgagc tgctcgccgt ggacctgggt
     6481 gtgcggggct ggcagctcag cctcacccac accgccacct cggcgggtgc ggtggtcgtc
     6541 gccctcgggt agcgccacac ccgaaccggg ccgcgacgag cgcgcgtccg aggcgtggac
     6601 cgcggcccag aacggtcagc gggggaggag ctcgggccgg aagcggtcct cggcgtggcg
     6661 gcgcagctcg gcggggatgg gcaccgcccg gcggctcacc gtgtcgagcg cgacgtcgac
     6721 gacctcgaag gccacgagca ggtcgccccg gtcgaggtcg tagccccaca tgacccggtg
     6781 ggtggtcttg tcccggatct ccaccacggc cccgaaggac tggatgcggt cgccccggcg
     6841 ggggaggcgg tggatgacca tgcggttctc catggtcgcc cacccgacga gctggccatc
     6901 ggggccgggg tgcagcgtgg gcccggtggc accctccagc ggctcgccac cccagagcag
     6961 gaacggagcg gccgccgggt cgtaggcgcc gtcgggccgg cactcgtcgg cctcgatggt
     7021 ccggggtcgg cgcatcgcca gcagctcgtc ggcctgcacg tcggcgaggg tgggtgcggt
     7081 ctcgagcggc gcggtgtcga gggcgatgct tcggggccgg ccgtgctcgg ggatgtcacc
     7141 ggtgcggagg tcgccagccg gcggccaggt cccgaccagg tcgtccccgt gccgggcctc
     7201 gaggcggtgc acgaagctgg cggcgaggtc gtcggtgtcg gtgttgcgca gctcgtggta
     7261 gagccgcagc acgccgccgc cgtgggacag cacggtgctg cgcacctcga gccgggcgtc
     7321 gaccagctgc tcgcggaagt gccgggtgta gatgtccacc gcctggaccg acaggtcgcc
     7381 ggcaccgccg aggtcccgca cgacggcgtc gctcgccgcc cgggcggcca cgccgtagta
     7441 ccgcacgttc atgtgcccga ggtggtcgat catctcctcg gtgacggtgg tgcggtgggt
     7501 ggtctgcagg tcgctcatgt ggtgctcggt ccctcctcgt cagccggacg acggtagcgg
     7561 tgggcccccg ggaacccgta ggccggtcgc cggtggcagg atcggtcggt gctccccgtc
     7621 gtccggatcg ccgagatgca ggccatcgac gccgacgcgc ccgaaccggt cgaggtcctg
     7681 atcgaccggg cgggcgccgc ggtggcccgc gccgccttgg acctcctcgg cgggggctac
     7741 ggccggcggg tggtcgtcct ggctggcccg ggcaacaacg gcgccgacgg acgggtggcg
     7801 gccgagaagc tgcgccgacg cggcctgcgg gtcgacgtga tcgagctggc cgacgcgccg
     7861 gacgtcctgc cggcggccga cctggtcatc gacgctgccc tcggcaccgg cgccagccgg
     7921 ccccaccacg cccccgaccc gggcgccgcc ctcgtgctcg ccgtcgacat cccctcgggg
     7981 atccacgggg acaccggcga ggccgtcggg tcgcccatgc gggccgacct caccatcacc
     8041 ttcgcggccc tgaagccggg cctgctcctc ggcgacggtc ccgcccacac cggcgccatc
     8101 cgcatcgccg acatcggcct ggacgtgtcc cgcgcccgga tgcacctcct cgacgacgcc
     8161 ggcgccgctg cgctcctgcc ccgccgggac accgccgcgc acaagtggca ccgctccgtg
     8221 ctggtggtcg ccggcagccc ggggatgacc ggtgccgcgt cgatggccgc cgaggccgcg
     8281 cagcgggccg gagccggcat ggtccgcctc gccacccccg gcggcgggca cggtgccgac
     8341 gtgccgcggg aggtcgtcac gctcgacgtc gaggaagccg gctgggccga ggcgctcgtc
     8401 gacgacccgg gcaaggtgca cgcgctcgcc gtcgggccgg gcatgggcac cgccatggcc
     8461 acccggcacc agatccgcac cctggtcgcc ggcatcgagg cgccggtggt ggtcgacggc
     8521 gacgggctca ccgcgctcgg caccgacgcg gcgtccgtca tcggacgccg tcgcgccggc
     8581 accgtgctca cgccccacga cggcgagttc gagcgcctcg ccggccatgc tcccggcacc
     8641 gaccgcgtcg ccgatgtggt cgccctcgcc gagcgcaccg gcgccgtcgt gctgctgaag
     8701 ggcccgacga cggtggtcgc cgcccccgac gggcagatcc gcctcgcccg cgccggcgac
     8761 gaccgcctgg ccacggccgg caccggcgac gtgctcaccg gcatcgtcgc cgccctcctc
     8821 gccggcggcc tcgaccccgt cgacgccgcc gcggtcggcg cccacgtgca cggcctcgcc
     8881 ggacgcgccg gaccccgcca cggcacgatg gcctcggatg tgatcgacgg catcccggcc
     8941 gcgctcgggc acctcctcgg ggacgagcgg tgaccgccga ccccgggtac ggcggatccg
     9001 tcgccgagat cgacctcgac gccatcgccc acaacgtggc cgtcctcgac gaccacgccg
     9061 gcgatgccca ggtgtgcgcc gtggtgaagg ccgacggcta cgggcacggc gcggtccccg
     9121 tcgcccgggc ggccctggcg gcgggtgcga cctggctcgg tgtggcccag gtgcaggagg
     9181 cccgggcgct gcgagccgcc ggcatcgacg cccccgtgct ggtgctgagc gaatgcgaca
     9241 cgtcggccga caccctcgac cacgccctgg cgatgggcct gcacctcgtc ggctaccggc
     9301 ccgggttcct cgacgccgtg tccgaacggg cccgggtgct cggcacgcga ccggcacccg
     9361 tccacctcaa ggtcgacacc ggcatgcgac gggtcgggtg cgaaccgcac caggcggtcg
     9421 acctggcccg ccgcatcgtc gaggacccgc acctggtgct cgccggcacc atgacccacc
     9481 tggccgtggc cgacgaaccc gggaacctcg gcaccgccgt gcagctcgac cggttcgagg
     9541 cggtgctcgc cggcttcgac gaggcgggca tcgatcccgg tgtccgccac gccgccaact
     9601 ccgccgccac cctcctgcac ccacgcgccc acctcgacct ggtccgaacc ggcatcgccg
     9661 tctacggcat cccgccgtcg ccggtgctct ccggggtcgc cgcgctgcgt cccgccatgc
     9721 gctggtcctc ctccgtccgc ttcgtgaagc cggtcgcgcg cggcgagggc gtgtcctacg
     9781 gccaccgcca cgtcttcgag gaggcgaccg tcgtggccac cgtgccggtc ggctacgccg
     9841 acggctaccg ccggcgcctc ggcctcgtcg ggggcgtggt gctcatccgc ggtcggcggt
     9901 gccccatcgt cggggtcgtc acgatggacc agctggtcgt cgacgtcgga ccggacggcg
     9961 atgtcgaggt cggcgacgag gtggtgctgg tcggcgccca gggagacgac gaggtcaccg
    10021 ccacggagct ggcgctgctc gtggacacca tcggctacga gatcacctgc gccgtcagct
    10081 cccgggttgc tcggcaccac cacggtggcc cggaggactc ggcctgatgg tcaccgacgt
    10141 cgacggcgtg cgggtcgggc actggaccga cgccgaggcc cgcaccgggt gcacggtcgt
    10201 gctgctcccc gagggcacgg tcgcgtccgg cgaggtccgg ggcggtgcgc ccgccacgcg
    10261 cgagttcgcc ctgctcgacc cgacccgcat ggtcgtccgg atggacgcgg tggtgctgag
    10321 cggcggatcg gccttcgggc tcgcggcggc ggacggcgtg atggcccacc tggccgaggc
    10381 cggtgtcggg ttcgagaccg tcggcggtcc ggtgcccatc gtcgtcggga tggcgctgta
    10441 cgacctggcg gtcggcgacc cctccgtgcg gcccaccgcc gagcacggcc gcgccgccgc
    10501 cgaggccgcg accgggggag gggtggacac cggcctggtc ggcgccggca ccggggcgac
    10561 cgtggcgaag tggcggggcc ctgaccaccg acggccgggc gggctcgtgg ccgccacgcg
    10621 ccggggtcgc gacggcctcg tcgtcgccgc ccgcgtggcc gtgaacgcgt tcggcgagcc
    10681 gggtgccgac cccggtgacg tggctgacgg cagcgtgggc ggctcggact tcaccgaggg
    10741 tgcccccggc acgaacacga ccatcggcgt ggtggtcacc aacgcccggc tcgacaaggt
    10801 cgcctgccac ctcctggccc agtcggccca ccacggcctc gcccgcagcg tctcgccctc
    10861 ccacacctcc gtcgacggcg acgcgttcgt cgcggcggcg
//
LOCUS       DATISU010000397        12243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_499333, whole genome shotgun sequence.
ACCESSION   DATISU010000397 DATISU010000000
VERSION     DATISU010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 12243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12243
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499333"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VLR27_15735"
     CDS             complement(<1..342)
                     /locus_tag="VLR27_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HSP04962.1"
                     /translation="MAIVERPSTSETLELEAGPRHATPEDALGVFKRPQGDTGWRSWL
                     FTVDHKRIGIMYGAAALFFFLVGGIEALIIRTQLAAPDQALISADLYNQLFTMHGVTM
                     IFLVVMPIGAAF"
     gene            complement(354..1559)
                     /gene="coxB"
                     /locus_tag="VLR27_15740"
     CDS             complement(354..1559)
                     /gene="coxB"
                     /locus_tag="VLR27_15740"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HSP04963.1"
                     /translation="MHRFRSTVGSARVRLFGLLALAGLVLSGCAMSLDGDTPQTTLHP
                     EGPAAEKIDGLSDLTFIIAAVVFVLVEVGVLFVAWKFRKRKDDTGDELPEQLHGNTKL
                     EIGWTALPAVILAFLAVFTVSTILDLAEDSEDATRIRVVGQQWWWSYDYDIDGDGVYA
                     DGDVHIPGIENPEEYETDIEVANEMVIPAGRPVTLEIEARDVIHSFWIPALNGKKDAV
                     PGRTHFLSLEADEPGTYVGQCTEYCGLSHAYMRMVVHALPQDEYDAWVEQQLAPANEP
                     QTAEEQAGLEVFVGRCASCHLVEGVNDDEYNGATVVPGAAPNLTHFASRGTYAGSIFN
                     LWQDTDGSDVIEWDEIGGELNRNQLEAWLRNPPAEKPMAADDNRGMPNLGLSEADIDS
                     LVAYLTSLD"
     gene            complement(1559..2386)
                     /locus_tag="VLR27_15745"
     CDS             complement(1559..2386)
                     /locus_tag="VLR27_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04964.1"
                     /translation="MITTGSKFFYGLAALLALAAVVYGYASGGGGVGPISAGYKGGVG
                     EHLGYGILLAAAVASLFAGFCVTAFRDADPEPTAALLGADTVPSATTAGSSFWPVVGA
                     FGVVLTVVGVVLNNIFFVAGLIALGAVAIEWTMQAWAERATGDPAVNREIRNRIMFPI
                     EVPLAGILAIAIVIVGYSRVFLAVSKLGAVWIALAIAATIFVIGTILSLRPRIRTDLV
                     AGVLAAAAVVTIGLGIFGAVAGEREFHDLSEEHAEEGGGEAEASDESTDEGASEESN"
     gene            complement(2397..3047)
                     /locus_tag="VLR27_15750"
     CDS             complement(2397..3047)
                     /locus_tag="VLR27_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HSP04965.1"
                     /translation="MATATATTTTPERLPLRLEDVPPAPPRRPRVLLIGTTMASAASI
                     ALFGGLLAAYFALRADVVGQGEQWLPDGTNIPLSPGNMTMVTLAMSLITAQWAVHAGK
                     LRDRGHAYFALGLTVLLGIAHVVQLGFLFTQWELPLNAADGESVTTQAVLLFTIMGLH
                     IAMVAAALIFFTLMLLRSLGGQFTGRDVEGLSAAALYWYVTVGIFVVLWYGILIVK"
     gene            complement(3047..4660)
                     /locus_tag="VLR27_15755"
     CDS             complement(3047..4660)
                     /locus_tag="VLR27_15755"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HSP04966.1"
                     /translation="MTVTETQAPPAATTTAPALEPPRPATGLAAVLGTGEHRTIGRLW
                     IFTSFAYLLVAGVAGALVGVEKVDTDGLGDVLGADVFGETLSLHGVAGTFLFLLPLFI
                     GLGTHVVPAQVGSATVAFPRAAAAAYWTYLLAGAVLIASFVADGGPFGADSRSVELFV
                     ASFIAVLIALVLGTICVATTAVALRTHGMGLHRTPLFAWSSLVTGVLVVLQLPVLAGL
                     MLLVYIDHRYGPTFLGGSEGVLDRIAWAWSQPSVYLFAIPVLGIIGDIVPVSARTRLS
                     KHRIAQGAIGAFAALAFGAWAMPGFGSDGAAPLLHVGDVPFYAFSILILFPALAFAGL
                     LGDTVRRGSLQVTSPLLWGIASLLMLLTGILNGILVAIEPFDLIQTTAQTAQIHYVLV
                     ASLLGFFAGLAHWAPRVLGTPVMEVASKGLAALGLLGTILLCLPDVISGFLDQAWRLQ
                     GVSGDEVDAVEALNIASIAGGVLLVLVAVAFVGLVLKAAAARDHVGDDPWEGNTLEWA
                     TADATPAITSEAPGSDARHGAGPSTTEAK"
     gene            complement(4833..5729)
                     /locus_tag="VLR27_15760"
     CDS             complement(4833..5729)
                     /locus_tag="VLR27_15760"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HSP04967.1"
                     /translation="MLVAATPSLRSKLGAYVSLTKPQIIELLLVTTVPVQIVAARGIP
                     SLWLIVTTVVGGTLAAGGAHAINMYVDRDIDALMARTRNRPLVTGVIEPRNALVFAVA
                     LEVAAFALLWSTVNLLSAVLAVVACLFYVFVYTLWLKRTSTQNIVIGGAAGAVPVLIG
                     WSSVTNSLDWAPVILFGVIFFWTPPHFWALAIKYKDDYAAADVPMMPVVADVRSTVRQ
                     MIAYTVVTWALTLLFAPVAGMGALYVAAAMVLGAIFTYQAYRLLRDASYARAMRLFGW
                     SITYVSLLFGAMALDQLVRSGF"
     gene            5828..6763
                     /locus_tag="VLR27_15765"
     CDS             5828..6763
                     /locus_tag="VLR27_15765"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HSP04968.1"
                     /translation="MRRPTISPTAYRRITLLALGLLGIIVVTGAGVRLTGSGLGCSTW
                     PSCEPGTLVPHDESGAHGVIESTNRYFTGLVSVGVILAVAGSFFRVPRRRDLTLWSWT
                     LVAGVLGQIVLGGLTVLFHLSPPFVMGHFLLSAVLVGCAVVLHHRAGEPDAGERRPVA
                     TPEVRLLAKVLLVAASVVLITGTVVTGSGPHGGDPDVERLPFFLPDVARIHAVAVWIL
                     LATTLWTLRLVHRGGASPSVERALKALVVLEVAQGAVGYLQYELGVPVGLVAVHILGS
                     MLVLGAAIVVLLRTSVVEPTRAPAPRTPDPVATGV"
     gene            6767..7078
                     /gene="clpS"
                     /locus_tag="VLR27_15770"
     CDS             6767..7078
                     /gene="clpS"
                     /locus_tag="VLR27_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HSP04969.1"
                     /translation="MVGSPRAGGTAPAEIQPDVDESSEVDTDTPWVAIVWNDPINLMS
                     YVSYVFRKLFGYSQAKADELMLDVHEKGRAVVTNGTREKVELDVFRLHEHGLWATMEQ
                     Q"
     gene            7081..7557
                     /locus_tag="VLR27_15775"
     CDS             7081..7557
                     /locus_tag="VLR27_15775"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HSP04970.1"
                     /translation="MGLFSGRRIKRIASGRYRIALPEPERELLSTLVPQLRELLTTDD
                     PSLNRLFPTAYADDPERDAGYHAMVRDELLEKRFHSLDVLERTIEGGEVDEETLSSWM
                     RALNDLRLVLGTRLDVSEDDDPMDIDPDDPLAPAWSIYHYLAALQSMIIDALSQEL"
     gene            7614..9236
                     /locus_tag="VLR27_15780"
     CDS             7614..9236
                     /locus_tag="VLR27_15780"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSP04971.1"
                     /translation="MRACLLTSPHPAFDVLVPGADAVAVDDGPDVVLVAGDTVGAPVE
                     VLADVTAAVERGAVLVCLGVPEGNGWAAITGVRPGEAVPGGEVFATVAARTPETARIT
                     GEFPVAGAFRWLEPVAEVATLLSSSIRFAHRPALVQRRLGAGRVVTCGFGADALLHGS
                     SGNRELVRILRRLVDGATAADRADLGVGVVGYGPYGGMGSLHGTAAAQTEGLALVAVV
                     DPADERRKAAEDEFPGVAAHESLDSMLADDAVDVVVVATPPNSHADLTLACLRAGKHV
                     AVEKPLCITTAEADLLLGTAADGDRMLTVHQNRRWDADFRALRRAVDAGLLGEVFNVE
                     TFVGGFEHPCRAWHSELSVSGGAIYDWGSHHLDWILLLLGGEPATVTTTRHKRVWHDI
                     TNDDQVRVRLAWADGREAEFLQSDIAGVRRPKLYVQGTEGTIAGHYRPVVTESVEHVH
                     GYQRHESHHAEAPVELTLARYEQGYGLTRTTLPPLPTEPWPFHRNVADHLLLGEALEV
                     DPVDVRRVIGVLEAAQRSGDEGGRTIELEPAG"
     gene            9325..10368
                     /locus_tag="VLR27_15785"
     CDS             9325..10368
                     /locus_tag="VLR27_15785"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSP04972.1"
                     /translation="MIDPRRSLTAGHDERTFRPATAPDVASSSWEPVRVGVIGASSMV
                     ARAAVVPAIEATPKAELVASASLAAGDDYDAVLADPDVEAVYVPLPNGMHREWVERCA
                     EAGKHVLCEKPLALTGSDARAMADACAANGVVLLEAYMTPYHPRSVALRDLVAAGRLG
                     RLLSVRSVFTFPHEEPADHRWHPLLGGGALADLGVYVLSPLFELAAAVGQDVPSSVTS
                     HAVVTEPDADGRCVDVTTAGEVRFPDGIVTTFECSFDAPEAQALEVTGTQGRVRVEHA
                     FTPDETDDELVLQRRDGTTDVIAGLGGDCYRGMVDHFCAVVRGLAESKRPPEVSVAFA
                     ELCDRLRSSQGQP"
     gene            10365..11228
                     /locus_tag="VLR27_15790"
     CDS             10365..11228
                     /locus_tag="VLR27_15790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP04973.1"
                     /translation="MTEPHAVEHVPVGDEVSLHLRRWDGGDRRPMVLVHGLASNARLW
                     DGVAAELHATGHPVAAVDQRGHGRSSKPDAGYEMATVVEDLRRLLDRLGWERPVVAGQ
                     SWGGNVVVELAATHPGLTAGVVGVDGGTIELAERFESWDDCATALRPPAMAGTPLADL
                     ERMLRSSHPDWPESGIQGTLACFEVRDDGTIAPWLTLERHLLVLRGLWEHRPSERYDR
                     ITEPVLLLGADPGPAESDWHRDKVRTLSAAAAALRDARVEWLRGDHDLHAQHPERVAE
                     LLARFADEIET"
     gene            11231..>12243
                     /locus_tag="VLR27_15795"
     CDS             11231..>12243
                     /locus_tag="VLR27_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04974.1"
                     /translation="MPRILTVMGSGETAPTMIKVHRRVMEAVGAEHPADAVLLDTPYG
                     FQENATDISTKAQEYFRASVGRPISVATWRGADDDTVTRERALAAIADASYVFAGPGS
                     PTYALAQWVGTPLPQLLADKLRRGGAVTFASAAALTLGVATVPVYEVYKAGATPAWAD
                     GLDLLSEVGLRVALIPHYDNAEGGHHDTRFCYLGERRLSVLEPDLPEGAWVLGVDEHT
                     ALVLDLDAGTAVVEGNGTVTVRRDGESAVLSAGTEIGLDELRPGTSAASPAPARDAAP
                     VADEAPVTNAGIGLAATTERLTADFDAALDARDPDGAVAAVLELEEAIVQWSADTLQS
                     DET"
BASE COUNT         1759 a         4348 c         4521 g         1615 t
ORIGIN      
        1 gaaggcggcg ccgatgggca tgacgacgag gaagatcatc gtcacgccgt gcatggtgaa
       61 cagctggttg tagaggtcgg ccgagatcag ggcctggtcc ggtgccgcca gctgggtccg
      121 gatgatcagc gcctcgatgc cgccgaccag gaagaagaac agcgcggcgg cgccgtacat
      181 gatgccgatg cgcttgtggt cgacggtgaa gagccaggag cgccacccgg tgtcgccctg
      241 cgggcgcttg aagacgccga gggcgtcctc gggtgtggcg tgccggggcc cggcctcgag
      301 ctcgagggtc tcggacgtgg aaggtcgttc gacgatggcc atggtgtggt tcctcagtcg
      361 agactggtca ggtaggcgac cagggagtcg atgtcggcct cgctgaggcc gaggttcggc
      421 atgccgcgat tgtcatctgc ggccatgggc ttctctgcgg gcgggttgcg cagccaggct
      481 tcgagctggt tgcggttcag ctcgccgccg atctcgtccc actcgatgac gtcgctgcca
      541 tcggtgtcct gccacaggtt gaagatggag ccggcgtagg tgccgcggct ggcgaagtgg
      601 gtcaggttgg gggcggcgcc cggcaccacc gtggcgccgt tgtactcgtc gtcgttgacg
      661 ccctcgacca ggtggcagct ggcgcagcgg cccacgaaca cctcgaggcc ggcctgctcc
      721 tcggcggtct ggggctcgtt cgccggcgcg agctgctgct cgacccaggc gtcgtactcg
      781 tcctggggca gggcgtggac gaccatgcgc atgtaggcgt gcgacaggcc gcagtactcg
      841 gtgcactggc ccacgtaggt gccgggctcg tcggcctcga gggagaggaa gtgcgtgcgc
      901 ccggggacgg cgtccttctt gccgttgagc gccgggatcc agaagctgtg gatcacgtcg
      961 cgagcctcga tctcgagggt gaccgggcga ccggcgggga tcaccatctc gttggcgacc
     1021 tcgatgtcgg tctcgtactc ctcggggttc tcgatgccgg ggatgtggac gtcgccgtcg
     1081 gcgtacacgc cgtcgccgtc gatgtcgtag tcgtaggacc accaccactg ctggccgacg
     1141 acccggatgc gggtggcgtc ctcgctgtcc tcggcgaggt cgaggatggt gctcaccgtg
     1201 aacacggcga ggaaggcgag gatgacggcc ggcagggcgg tccagccgat ctccagcttg
     1261 gtgttgccgt gcagctgctc gggcagctcg tcgccggtgt cgtccttgcg cttgcggaac
     1321 ttccaggcga cgaagaggac gccgacctcg acgaggacga agaccaccgc ggcgatgatg
     1381 aacgtgaggt cggagaggcc gtcgatcttc tcggcggccg gaccctcggg gtggagggtg
     1441 gtctggggcg tgtcgccgtc gaggctcatg gcgcagcccg agaggaccag cccggcgagc
     1501 gcgaggaggc cgaagaggcg gacccgggcg ctgccgacgg tggaacggaa gcggtgcatc
     1561 agttgctctc ctcggaggcg ccttcgtcgg tgctctcgtc gctggcctcg gcctcgccgc
     1621 cgccttcttc ggcgtgctcc tcggagaggt cgtggaactc gcgctcgccg gccacggcac
     1681 cgaagatgcc gaggccgatg gtgaccacgg cggccgcggc gagcacgccg gcgacgaggt
     1741 cggtgcggat gcggggacgg agcgagagga tcgtgccgat gacgaagatg gtggccgcga
     1801 tcgccagcgc gatccagaca gcccccagct tgctcacggc gaggaacacc cgcgagtagc
     1861 cgacgatgac gatcgcgatg gcgaggatgc cggccagcgg gacctcgatg gggaacatga
     1921 tccggttgcg gatctcgcgg ttgacggcgg ggtcgccggt ggcccgctcg gcccaggcct
     1981 gcatggtcca ttcgatggcg accgcaccca gggcgatgag cccggcgacg aagaagatgt
     2041 tgttgagcac gacgccgacg accgtgagga ccacgccgaa ggcgcccacg accggccaga
     2101 aggacgagcc ggcggtggtg gcgctgggga cggtgtcggc gcccagcagg gcggcggtgg
     2161 gctcgggatc ggcgtcgcgg aaggcggtga cgcagaagcc ggcgaagagg gaggcgaccg
     2221 cagcggcgag caggatgccg tagccgaggt gctcgccgac gccgcccttg tagcccgcgc
     2281 tgatcgggcc gacgcccccg ccgccgctgg cgtagccgta gacgacagca gccagggcga
     2341 gcagcgccgc gaggccgtag aagaacttgg agccggtggt gatcatcggt gctcgcctac
     2401 ttcacgatca ggatgccgta ccagaggacc acgaagatcc cgacggtcac gtaccagtac
     2461 agcgcggcag cggacaggcc ctcgacgtcg cggccggtga actggccacc gaggctgcgc
     2521 aggagcatga gggtgaagaa gatcagggcg gcagcgacca tggcgatgtg gaggcccatg
     2581 atcgtgaaga gcagcacggc ctgcgtggtg acgctctcgc cgtcggcggc gttgagcggg
     2641 agctcccact gcgtgaacag gaagccgagc tgcaccacgt gggcgatgcc gagcagcacc
     2701 gtgaggccga gggcgaagta ggcatggccc cggtcgcgca gcttgccggc gtgcacggcc
     2761 cactgcgccg tgatcagcga catggccagc gtcaccatgg tcatgttgcc cggggacagc
     2821 gggatgttgg tgccgtcggg gagccactgc tcgccctggc cgaccacgtc ggcgcggagg
     2881 gcgaagtacg cagcgagcag gccgccgaac agggcgatcg aggccgccga ggccatcgtc
     2941 gtgccgatga gcagcacccg gggccggcga ggcggtgccg gcggcacgtc ctccaggcgc
     3001 agcgggagcc gctcgggggt ggtggtggtc gcggtggccg tggccatcac ttcgcctccg
     3061 tggtcgaggg gccggcgccg tgacgggcgt cggaccccgg ggcctccgag gtgatcgcgg
     3121 gcgtcgcgtc ggcggtcgcc cactcgaggg tgttgccctc ccaggggtcg tcgccgacgt
     3181 ggtcccgggc ggcggcggcc ttgaggacga gtccgacgaa ggcgacggcc acgagcacca
     3241 ggagcacgcc gccggcgatg ctggcgatgt tgagcgcctc caccgcgtcg acctcgtcgc
     3301 cgctcacgcc ctgcaggcgc caggcctggt cgaggaagcc gctgatgacg tcggggaggc
     3361 agagcaggat cgtgccgagc aggccgaggg cggcgaggcc cttggaggcg acctccatca
     3421 ccggcgtgcc gaggacccgg ggcgcccagt gggcgaggcc ggcgaagaac ccgagcagcg
     3481 aggcgaccag cacgtagtgg atctgggcgg tctgggccgt ggtctggatc aggtcgaagg
     3541 gctcgatggc caccaggatc ccgttgagga tgccggtgag gagcatgagc agcgaggcga
     3601 tgccccacag gagcgggctg gtgacctgca gggatccccg gcgcacggtg tcgccgagca
     3661 ggccggcgaa ggcgagggcg gggaacagga tcaggatcga gaaggcgtag aagggcacgt
     3721 cgccgacgtg gagcagcggg gcggcgccgt cggacccgaa gcccggcatg gcccaggcac
     3781 cgaaggccag ggcggcgaag gcaccgatgg cgccctgggc gatccggtgc ttgctgaggc
     3841 gggtgcgggc cgagaccggg acgatgtcgc cgatgatgcc gaggaccggg atggcgaaca
     3901 ggtacacgct gggctgggac caggcccagg cgatgcggtc gaggacgccc tcgctgccgc
     3961 cgaggaaggt cgggccgtag cggtggtcga tgtagacgag cagcatcagc ccggcgagca
     4021 ccgggagctg gaggaccacg aggacgccgg tgacgaggct ggaccaggcg aacagcggcg
     4081 tgcggtgcag gcccatgccg tgcgtgcgca gggcgacggc cgtggtggcg acgcagatgg
     4141 tgccgaggac cagggcgatc aggacggcga tgaaggaggc cacgaacagc tcgacggagc
     4201 ggctgtcggc gccgaagggg ccgccgtcgg ccacgaacga ggcgatcagc accgcaccgg
     4261 ccagcaggta ggtccagtag gcggcagcag cagcccgggg gaaggcgacg gtggccgatc
     4321 cgacctgcgc cggcaccacg tgggtgccga ggccgatgaa cagcggcagc aggaacagga
     4381 aggtgccggc cacgccgtgg aggctcaggg tctcgccgaa gacgtcggcg ccgagcacgt
     4441 cgccgaggcc atcggtgtcg accttctcga cgcccaccag ggcaccggcc acgccggcga
     4501 ccaggaggta ggcgaaggag gtgaagatcc acagccgccc gatggtgcgg tgctcgcccg
     4561 tgcccagcac ggccgcgagg ccggtggcgg gacgaggcgg ctccagtgcc ggagcggtgg
     4621 tggtggccgc aggcggcgct tgggtttcag tcacggtcat cgctgcgggt ttctcctccg
     4681 gccgggcgac gtcgcggcgt cggaaccggg aacagtccgg gtcggctgcc gaatccgagc
     4741 ggacagtacc caacgtccac ttgacggcac gaaccaggtc tcaggggtgg attcgtcgtg
     4801 accggggacc ctcgacgcgc gcaccgacac ggtcagaagc cgctgcgcac cagctggtcc
     4861 agcgccatgg caccgaacag gaggctcacg tacgtgatcg accacccgaa gaggcgcatg
     4921 gcccgggcgt aggaggcgtc ccggagcagc cggtacgcct ggtaggtgaa gatcgcgccg
     4981 agcaccatcg cggcggcaac gtacagggcg cccatgccgg cgaccggggc gaacagcagg
     5041 gtcagggccc aggtgacgac cgtgtaggcg atcatctggc gcaccgtgga gcgcacgtcg
     5101 gcgaccaccg gcatcatcgg cacgtcggcc gcggcgtagt cgtccttgta cttgatggcc
     5161 agcgcccaga agtggggcgg ggtccagaag aagatgaccc cgaacaggat caccggcgcc
     5221 cagtcgaggg agttggtgac gctggaccag ccgatgagca ccggcaccgc accggcggcg
     5281 ccgccgatga cgatgttctg cgtcgaggtg cgcttcagcc acagcgtgta gacgaagacg
     5341 tagaagaggc aggcgaccac ggccagcacg gccgacagca ggttgaccgt ggaccacagc
     5401 agcgcgaacg ccgcgacctc gagggcgacg gcgaacacga gcgcgttgcg gggctcgatg
     5461 acgccggtga ccagcgggcg gttgcgggtg cgggccatca gggcgtcgat gtcgcggtcg
     5521 acgtacatgt tgatggcgtg ggcaccgccg gcggccaggg tgccgccgac caccgtggtg
     5581 acgatcagcc acagcgacgg gatgccccgg gcggccacga tctggacggg caccgtggtc
     5641 accagcagca gctcgatgat ctgcggcttc gtcagcgaga cgtaggcgcc gagcttcgac
     5701 cggagcgagg gagtggcggc cacgagcatg acccggtgag cctacgtccg gtgggacggg
     5761 ggggaccaag gcgccccggc cccgcggcgc ctggcacccg cggcaccgga gcggccacgc
     5821 tggaggggtg cgtcgcccga cgatctcccc cacggcgtac cgccggatca ccctgctcgc
     5881 gctggggctg ctgggcatca tcgtggtcac cggcgccggc gtgcgcctca ccggatcggg
     5941 cctgggctgc tccacctggc cgagctgcga gcccggcacc ctggtccccc acgacgagtc
     6001 cggcgcgcac ggggtcatcg agtccacgaa ccgctacttc accggcctcg tctccgtcgg
     6061 cgtgatcctc gccgtcgccg gctccttctt ccgcgtgccc cgccggcggg acctcaccct
     6121 gtggtcctgg accctggtcg ccggcgtgct gggccagatc gtgctcggtg gcctcacggt
     6181 gctgttccac ctgtcgccgc cgttcgtgat gggacacttc ctgctctcgg cggtgctggt
     6241 cggctgcgcc gtcgtgctgc accaccgcgc cggcgagccc gacgcgggcg agcgccgccc
     6301 ggtggccacc ccggaggtcc gcctcctggc caaggtgctc ctcgtcgctg catccgtcgt
     6361 cctgatcacc ggcaccgtgg tcaccggctc gggcccccac ggcggcgatc cggacgtcga
     6421 gcgcctcccc ttcttcctgc ccgacgtcgc gcggatccac gccgtggccg tgtggatcct
     6481 gctcgccacc accctgtgga cgctgcgcct ggtgcaccgg ggcggcgcgt ccccgtcggt
     6541 ggagcgggcg ctgaaggcgc tggtcgtgct cgaggtcgcc cagggcgccg tcggctacct
     6601 ccagtacgag ctgggcgtgc ccgtcggcct cgtcgccgtc cacatcctcg ggtcgatgct
     6661 cgtgctcggc gccgccatcg tcgtcctgct ccgcacgagc gtggtcgaac cgacccgggc
     6721 ccccgccccc cgcacccccg accccgtcgc cacgggggtc tgaggcgtgg tcggctcccc
     6781 ccgcgccggc ggcaccgccc cggccgagat ccagcccgat gtcgacgaga gcagcgaggt
     6841 cgacaccgac acgccctggg tcgccatcgt ctggaacgac ccgatcaacc tgatgtccta
     6901 cgtgtcctac gtgttccgca agctgttcgg ctactcccag gccaaggctg acgagctgat
     6961 gctcgacgtg cacgagaagg gccgggccgt ggtcaccaac gggacccggg agaaggtgga
     7021 gctcgacgtg ttccggctcc acgagcacgg cctgtgggcg accatggagc agcagtagcg
     7081 gtggggctgt tctccggtcg acggatcaag cggatcgcct ccggccgcta ccgcatcgcc
     7141 ctgcccgaac ccgagcggga gctgctgtcg accctcgtgc cccagctgcg cgagctgctc
     7201 accaccgacg acccgtccct gaaccggctc ttccccacgg cctacgccga cgaccccgag
     7261 cgcgacgccg gctaccacgc aatggtgcgc gacgagctgc tcgagaagcg gttccactcc
     7321 ctcgacgtcc tcgagcgcac catcgagggt ggcgaggtcg acgaggagac gctgtcgtcg
     7381 tggatgcggg cgctcaacga cctgcgcctc gtgctcggca cccgcctcga cgtgtccgag
     7441 gacgacgacc ccatggacat cgaccccgac gatccgctcg caccggcctg gtcgatctac
     7501 cactacctcg ccgccctcca gagcatgatc atcgacgccc tcagccagga gctctgaccg
     7561 gccggcgggc ccgacggcac gccggatcga tgtcgctcta cgctccgacg cccatgcgcg
     7621 cctgcctgct cacctcgccc cacccggcgt tcgacgtcct ggtgcccggc gcggacgcgg
     7681 tcgccgtcga cgacggcccc gatgtcgtgc tcgtggcggg cgacactgtc ggcgccccgg
     7741 tcgaggtgct ggccgacgtc accgccgccg tcgagcgcgg tgcggtgctg gtctgcctcg
     7801 gggtgcccga gggcaacggc tgggccgcga tcaccggcgt ccgtcccggg gaggccgtcc
     7861 ccggcggtga ggtcttcgcc accgtggcgg cccgcacgcc ggagaccgcc cgcatcaccg
     7921 gggagttccc ggtggccggg gcgttccgct ggctggagcc ggtggccgag gtcgccacgc
     7981 tgctcagctc cagcatccgc ttcgcgcacc gcccggcgct ggtgcagcgg cgcctcgggg
     8041 cgggccgggt cgtcacctgc gggttcggtg ccgacgccct cctgcacggc tcgtcgggca
     8101 accgcgagct cgtccggatc ctgcgccggc tggtcgacgg ggccacggcg gccgaccggg
     8161 ccgacctcgg cgtcggggtc gtgggctacg gcccctacgg cggcatgggc tcgctgcacg
     8221 gcaccgccgc ggcccagacc gaggggctgg cgctggtcgc ggtcgtggac ccggccgatg
     8281 agcgtcggaa ggccgccgag gacgagttcc ccggcgtcgc cgcccacgag tcgctcgact
     8341 cgatgctggc cgacgacgcg gtcgacgtcg tggtggtcgc cacgccgccg aactcccacg
     8401 ccgacctcac cctcgcctgc ctccgcgccg gcaagcacgt ggcggtcgag aagccgctgt
     8461 gcatcacgac cgccgaggcc gatctgctcc tggggaccgc ggccgatggc gaccgcatgc
     8521 tgaccgtgca ccagaaccgc cgctgggacg ccgacttccg ggcgctgcgg cgcgcggtcg
     8581 atgccggcct gctcggcgag gtgttcaacg tggagacctt cgtcggcggc ttcgagcacc
     8641 cgtgccgggc gtggcactcc gagctcagcg tgtccggcgg cgccatctac gactggggca
     8701 gccaccacct cgactggatc ctcctgctgc tcggcggcga gccggccacc gtcaccacca
     8761 cccgacacaa gcgggtgtgg cacgacatca ccaacgacga ccaggtccgg gtccggctgg
     8821 cgtgggccga cggccgcgag gccgagttcc tccagtccga catcgccggc gtgcggcggc
     8881 cgaagctgta cgtgcagggc accgagggca cgatcgccgg ccactaccgg cccgtcgtca
     8941 ccgagtccgt cgagcacgtc cacggctacc agcgccacga gtcccaccac gccgaggcgc
     9001 cggtggagct cacactcgcc cgctacgagc agggctacgg cctgacccgg accacgctgc
     9061 cgccgctgcc gaccgagccg tggccgttcc accgcaacgt cgccgaccac ctcctgctcg
     9121 gcgaggccct cgaggtcgac ccggtcgatg tccgccgggt gatcggcgtg ctcgaggcgg
     9181 cccagcgctc cggggacgag ggcggccgca cgatcgagct cgagcccgcc gggtgagccc
     9241 tcaggagccg agccgccagg cgaggcgacc cagcagcacg gtgccctacg gcggcggagc
     9301 cgccagaccg gagtgcggaa ggacgtgatc gatccgcgta ggtctctgac ggccggtcac
     9361 gacgagcgca cgttccggcc ggccaccgcg cccgacgtgg cctcgtcgag ctgggagccg
     9421 gtgcgcgtcg gcgtgatcgg ggcgtcctcg atggtcgccc gggccgcggt cgtgcccgcc
     9481 atcgaggcca cgccgaaggc cgagctggtg gcgtcggcct cgctcgccgc cggcgacgac
     9541 tacgacgccg tgctggccga cccggacgtc gaggcggtct acgtcccgct gccgaacggc
     9601 atgcaccgcg agtgggtcga gcgctgcgcc gaggccggca agcacgtcct gtgcgagaag
     9661 ccgctggccc tcacgggctc cgacgcccgg gcgatggccg acgcctgcgc cgccaacggc
     9721 gtcgtgctgc tcgaggcgta catgacgccg taccaccccc gctcggtggc gctgcgggac
     9781 ctcgtcgcgg ccggccgcct cggtcgcctg ctgtccgtgc ggtcggtgtt cacgttcccc
     9841 cacgaggagc cggcggacca ccggtggcac ccgctgctcg gcggcggagc gctcgccgac
     9901 ctcggcgtgt acgtcctcag ccccctgttc gagctcgccg cggccgtcgg ccaggacgtc
     9961 cccagctcgg tcaccagcca cgccgtggtc accgaacccg acgccgacgg ccgctgcgtg
    10021 gacgtcacca cggcgggcga ggtccgcttc cccgacggga tcgtcaccac cttcgagtgc
    10081 tccttcgacg cgccggaggc ccaggccctc gaggtcaccg ggacccaggg tcgggtgcgg
    10141 gtcgagcacg ccttcacgcc cgacgagacc gacgacgagc tcgtgctcca gcgccgggac
    10201 gggaccaccg acgtcatcgc cggcctcggc ggagactgct accggggcat ggtcgaccac
    10261 ttctgcgcgg tggtccgcgg cctggccgag tccaagcgcc ccccggaggt ctcggtcgcc
    10321 ttcgccgagc tgtgcgaccg gctgcgatcg tcgcagggcc aaccgtgacc gagccgcacg
    10381 cggtcgagca cgtccccgtc ggcgacgagg tctcgctgca cctgcgtcgc tgggacggcg
    10441 gcgaccgccg gccgatggtg ctcgtgcacg ggctcgcgtc caacgcccgg ctgtgggacg
    10501 gcgtcgcggc cgagctccac gccaccgggc acccggtggc cgccgtggac cagcggggcc
    10561 acgggcgatc gtccaagccc gacgctggct acgagatggc gaccgtcgtc gaggacctcc
    10621 ggcgcctgct cgaccgactc ggctgggagc gcccggtcgt cgccggtcag agctggggcg
    10681 gcaacgtcgt cgtcgagctg gccgccaccc acccgggcct caccgccggc gtggtcgggg
    10741 tggacggcgg caccatcgag ctcgccgagc gcttcgagtc ctgggatgac tgcgccaccg
    10801 ccctccgacc cccggcgatg gccggcaccc cactggccga cctcgagcgc atgctgcgct
    10861 cgtcgcaccc ggactggccc gagtcaggca tccaggggac gctcgcctgc ttcgaggtgc
    10921 gtgacgacgg caccatcgcg ccgtggctga ccctcgagcg ccacctgctc gtcctccggg
    10981 gcctgtggga gcaccggccc tcggagcgct acgaccggat caccgagccc gtgctcctcc
    11041 tcggcgccga ccccggacca gccgagagcg actggcaccg ggacaaggtc cgcaccctct
    11101 cggccgcagc cgccgcgctc cgcgacgccc gggtcgagtg gctccgtggc gaccacgacc
    11161 tccacgccca gcacccggag cgggtggccg agctgctggc ccgcttcgcc gacgagatcg
    11221 agacctgacc gtgccccgca tcctcaccgt catggggtcg ggcgagaccg cccccaccat
    11281 gatcaaggtc caccgccgcg tcatggaggc cgtcggcgcc gaacacccgg ccgacgccgt
    11341 gctcctggac acgccgtacg gcttccagga gaacgccacc gacatctcga ccaaggccca
    11401 ggagtacttc cgggcctcgg tcggccgccc gatctcggtg gcgacctggc gcggcgccga
    11461 cgacgacacg gtgacccggg agcgggccct ggccgccatc gccgacgcca gctacgtgtt
    11521 cgccggcccg gggagcccga cctacgccct ggcccagtgg gtcggcaccc cgcttcccca
    11581 gctgctcgcc gacaagctcc ggcggggtgg ggccgtgacg ttcgcctcgg ccgcggcgct
    11641 caccctgggt gtcgcgacgg tccccgtcta cgaggtctac aaggccggtg ccaccccggc
    11701 ctgggccgac gggctcgacc tgctgtccga ggtcggcctg cgcgtggcgc tcatccccca
    11761 ctacgacaac gccgagggcg gccaccacga cacccggttc tgctacctgg gcgagcggcg
    11821 cctgtccgtg ctggagccgg acctgcccga gggggcgtgg gtgctcgggg tcgatgagca
    11881 caccgccctg gtcctcgacc tcgacgccgg gaccgccgtg gtcgagggca acggcaccgt
    11941 caccgtccgg cgcgacggcg agtccgccgt cctgtccgcc ggcaccgaga tcggcctcga
    12001 cgagctccga cccgggacca gcgcggcatc gcccgcccct gcacgtgacg ctgctcccgt
    12061 cgccgacgag gccccggtca ccaacgccgg catcggcctg gccgccacga ccgagcgcct
    12121 caccgccgac ttcgacgccg ccctcgacgc ccgcgacccc gacggggccg tcgccgccgt
    12181 gctcgagctg gaggaggcca tcgtccagtg gtcggccgac acgctccaga gcgacgagac
    12241 cga
//
LOCUS       DATISU010000398         1568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_266712, whole genome shotgun sequence.
ACCESSION   DATISU010000398 DATISU010000000
VERSION     DATISU010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266712"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VLR27_15800"
     CDS             complement(<1..327)
                     /locus_tag="VLR27_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HSP04975.1"
                     /translation="MANRDLLAASALFSGLTEDELDAVVSAATTRELRRGDVLFSEGD
                     DSEELFLVEDGRVAMSQRSADGRESVIALMERGDLFGEMPLFDGLGRSTEARALEPSI
                     VTVVPYE"
     gene            424..1089
                     /locus_tag="VLR27_15805"
     CDS             424..1089
                     /locus_tag="VLR27_15805"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HSP04976.1"
                     /translation="MPAPTAAAPVWTVVVAGGGGTRFGGPKQYVELAGRRTIDWSLAA
                     ARSVSDGIVVVVPADDVDEPVDDADAVVAGGDTRSASVRNGLAAVPDDAGVVLVHDAA
                     RPAASPALFRAVVEAVRNGAEAVVPGVAVTDSLRRRSGGAVDRDELVAVQTPQGFDAA
                     ALRAAHASGGDASDDASLVEAGGGTVAVVLGETTNVKLTHPGDRVALEATLASRSGEG
                     APA"
     gene            1086..1568
                     /gene="ispF"
                     /locus_tag="VLR27_15810"
     CDS             1086..1568
                     /gene="ispF"
                     /locus_tag="VLR27_15810"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HSP04977.1"
                     /translation="MTGRVGMGFDVHPLGDDPERPMVLGGVTFPGPGLVGHSDADVIA
                     HACADALLGAAGLGDIGSHFPDTDPALAGADSIELLRAVGDEIRAAGWLVANVDCSVV
                     CDEPKIGPSREQMQQRLSDAAGGPVTVKGKRPEGLGALGRHEGIACWAVAMVEREDPR
                     "
BASE COUNT          207 a          569 c          581 g          211 t
ORIGIN      
        1 ctcgtagggg accacggtga cgatcgaggg ctcgagggca cgggcctcgg tggaccggcc
       61 gagcccgtcg aacaggggca tctcgccgaa caggtcgcct cgctccatca gcgcgatgac
      121 ggactcccgc ccgtcggccg agcgctgcga catggccacg cgcccgtcct cgacgaggaa
      181 cagctcctcg gagtcgtcgc cctcggagaa caggacgtcg ccccggcgca gctcccgggt
      241 ggtggccgcg ctgaccactg cgtcgagctc gtcctcggtg agacccgaga agagcgcgga
      301 ggctgcgagg aggtcacggt tggccacgag gtccgaatct atgccaaacg tcaccggatc
      361 cggcgcgagg acgccatccc ggccggatgc gaggaggtgc cggctgctgc gtacgctccg
      421 tgcatgcctg cccccaccgc tgccgccccg gtgtggaccg tggtcgtcgc cggcggcggc
      481 ggcacgcggt tcggcgggcc gaagcagtac gtcgagctgg ccggtcgtcg cacgatcgat
      541 tggtccctcg ccgccgcccg ctcggtgtct gacggcatcg tcgtggtcgt gcccgccgac
      601 gacgtggacg aaccggtcga cgacgccgac gcggtcgtgg ccggcgggga cacccgctcc
      661 gcctcggtcc gcaacggcct cgccgctgtg cccgacgacg ccggtgtggt cctcgtgcac
      721 gacgcggccc gtcctgcggc cagcccggcg ctgttccggg cggtcgtgga ggccgtgcgc
      781 aacggtgccg aggccgtggt ccctggggtc gcggtcaccg actcgctccg ccgccggtcc
      841 gggggagcgg tggaccgcga cgagttggtg gccgtccaga cgccgcaggg cttcgacgcc
      901 gcggccctgc gcgccgctca tgcatccggt ggcgacgcct cggacgatgc gtccctggtc
      961 gaggcagggg gcgggacggt ggcggtcgtg ctgggcgaga ccaccaacgt caagctcacc
     1021 caccccggcg accgcgtcgc gctcgaagcg acgctcgcgt cgcgcagcgg cgaaggagca
     1081 ccggcatgac cggccgcgtc ggcatgggct tcgatgtcca ccccctcggt gacgatcccg
     1141 agcggccgat ggtcctcggc ggcgtcacct tccccggtcc ggggttggtc ggccacagcg
     1201 acgccgacgt catcgcccac gcctgcgcgg acgccctgct gggtgcggca gggctcggcg
     1261 acatcggttc ccacttcccc gacaccgatc cggcgctcgc cggcgccgac agcatcgagc
     1321 tgctccgggc cgtcggcgac gagatccggg cggccggttg gctcgtcgcc aacgtcgact
     1381 gctcggtcgt ctgcgacgaa cccaagatcg gaccgagccg ggagcagatg cagcaacggc
     1441 tgagcgatgc ggccggcggt ccggtcaccg tgaagggcaa gcgtcccgag ggcctcggcg
     1501 ccctgggtcg ccacgagggc atcgcctgct gggcggtcgc catggtcgaa cgagaggatc
     1561 cacgatga
//
LOCUS       DATISU010000399        21663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_499593, whole genome shotgun sequence.
ACCESSION   DATISU010000399 DATISU010000000
VERSION     DATISU010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 21663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21663
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499593"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VLR27_15815"
     CDS             complement(<1..363)
                     /locus_tag="VLR27_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04978.1"
                     /translation="MSRLDEVGEQDGWRCWLCDEPVDPDMSVNDARGPSIDLRTTKAK
                     AKKKGADGQERLAHRGCNTGKGAKDPVVPWPEHLFVVDPAVILTAVDRLERKGGREVM
                     ARCPTADDADAAAAWLTDR"
     gene            468..1868
                     /gene="fumC"
                     /locus_tag="VLR27_15820"
     CDS             468..1868
                     /gene="fumC"
                     /locus_tag="VLR27_15820"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HSP04979.1"
                     /translation="MASESRTETDSMGGIDVAADRYWGAQTQRSIGNFPIGRDRFVWG
                     RPIIEAFGLVKWAGARANTQLGELDEEISELVQRAADEVIAGDLDDHFPLVVWQTGSG
                     TQSNMNANEVISNRAIEMAGGEIGSKSPVHPNDHVNRGQSSNDTFPTAMHIAIVLELH
                     RQLYPGVMALRDALRDKAVEFADVVKVGRTHLQDATPITMGQEIGGWVHQIDWALEQV
                     RRSEEGLLELAIGGTAVGTGLNAHPRFGAVAAAELAERTGHEFRVAGNRFAALSAHDA
                     LVATSGALRTLAGALMKIANDIRWEASGPRNGIGELRIPENEPGSSIMPGKVNPTQSE
                     ALTMVAVQVFGNDAAVAFGGSQGNFQLNVFKPVMAHNVLESIALLGDASASFEEHCAR
                     GLEPDHDRIRHNLEQNLMVVTALNPHIGYDKGAQIAKKAHAEGTTLREAAIGSGHLTA
                     EQFDEWVDPMAMTRAD"
     gene            complement(1873..2769)
                     /locus_tag="VLR27_15825"
     CDS             complement(1873..2769)
                     /locus_tag="VLR27_15825"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HSP04980.1"
                     /translation="MTPSVDGPLELRCAPWTLDQGVDPIGSAVEIDRLVTVEVPLPWP
                     ADVADHPWIADLELDPGTRMQTIVPEVARTDGTVLVTRWTADGARFTGTDWLVPAVDV
                     PDALVVVASGGDPDVPGEGAPDEILVCGHGARDRCCGGSGTRLAVEARAALPEVRIRR
                     TSHLGGHRFAPTALSLPDGRVWAFLDVEAIAGIVDRTLHPIDARDHYRGHVGLDPWAQ
                     VVEAVGLSEHGWAALDVDHLEADVDVDADGTSATVTLRWDGATGAGERTARVGVRRRY
                     PVLQCGLPPEAAKKDAPEYELL"
     gene            complement(2885..3163)
                     /locus_tag="VLR27_15830"
     CDS             complement(2885..3163)
                     /locus_tag="VLR27_15830"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HSP04981.1"
                     /translation="MLWFLLVLFFFGIIFGAIARLLVPGRDPMGIVGTWALGVAGSFV
                     GGFLGYLIFGADLDDGAVQMGGIFGSIIGSVVLLLVVRSVRGGRRRTA"
     gene            complement(3251..5956)
                     /locus_tag="VLR27_15835"
     CDS             complement(3251..5956)
                     /locus_tag="VLR27_15835"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HSP04982.1"
                     /translation="MHAGPDQLARGVVVADGASPPAPWAGVPVVVVDEDVLAQPGAVV
                     ERLHAAWAGRERVVVDLRVDPVRFRRPVSHTDVPWELSPTFEPWLDRLHFLVWHNSYD
                     ARGDEVVWWWGRKAARIDGTAVDELPAGHDPVEGDVLLPDGTVAWIDGGPPDDVDPAT
                     LAPAVLVRAESVDAGHLRPVPAWVAPSAELAPDQLAAVAHRGGPARIIAPAGSGKTRV
                     LTERLRHLLADRGHERDLLLAVAYNRKAQEEMAGRTAGLGARIQTLNALGYELIGRHA
                     GRRPQMLDEREVRRRLEPHLPKLQHRLNTDPMAPYLEGLSVIRLGLRDPDEVELEADA
                     PGLAAAVGPYREGLRRDSVLDFDEQIIHAVELLLSDGEFRRREQNRHRHLLVDEFQDL
                     TPAHVLLLRLLAAPTYDVFGVGDDDQVIYGHAGASPQFLLRFADLFPGAHEHALQVNY
                     RCPPAVVDAARHLLGYNDERVAKTITAARPAGPGDALTVTQHPPQEGATRVVEAVRAA
                     VEATGDPGQVAVLTRTNSLLLAPHVALHGAGIPIASVLRRDVLQRVGLRAALAWLRVA
                     TDPGSIASTDLTEIRRRPSRGFPNWVDKWLGRCRSGHEVAKAAERIDDARVADKLLDL
                     AADIDRLGDLARGATTREILQAIRDDIGLGGAMELLDGRPGAEGSSHLDDLDALLQVA
                     DLQPDPDEFEAWLRGALDGPRASDGDPTAEAAAGVTLSTVHRVKGREWPHVIVFGADA
                     GIFPHRLAEDVEEERRVFHVAITRGIEAVTVLADDDRPSPFLAELDGTASKQAPRRTA
                     RTGAATGSRAAGSAKAAKAAKTSEPDLEGEDLVLFEELRRWRSEVAKRQGGPAFIIFG
                     DAPLKDIAKARPADLRALSRIKGVGPSKLDSYGDDVLEIVARFAT"
     gene            6153..7574
                     /locus_tag="VLR27_15840"
     CDS             6153..7574
                     /locus_tag="VLR27_15840"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HSP04983.1"
                     /translation="MSIELQEQGHEKTAKRDGWLVLVATFSALAMIVMLFAAGVAVRG
                     GDDDGGGGAPVATETIDVTLGDLFVEPSSLTMPAGATLTLNVTNDGAMPHDLKVGGTD
                     GTAMLDPGASETITIGPFDASVEAWCTVPGHREGGMNMMITVEGEAGGETAVAAAEGD
                     AAEVGGAALGGAEIDFNAAPEGDWQPFDPALAPAPGGTEHEVTWSMTEEVMEIAPGVT
                     QEVWTFDGQVPGPTLRGKVGDIFTVTIVNDGEMGHSLDFHASKVAWNDEMRTIQPGEE
                     LVYQFEAKHAGAFMYHCGTAPTLHHIGNGMHGAIIIDPPDLAPVDHEFVFVQHEYYTG
                     GEGEPGDLGKMQRDEWDAVVFNGYVNQYVHAPIDGIEVGERVRAWVVDNGPSENSSWH
                     VVGTVFDTLYKEGAYQLRPGDGNQGGAQALDLQPAQGGFVEFTFDEEGAYPFVTHKFS
                     NAGKGAMGLFVAGDPDLEGIGGH"
     gene            complement(7742..8644)
                     /locus_tag="VLR27_15845"
     CDS             complement(7742..8644)
                     /locus_tag="VLR27_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04984.1"
                     /translation="MRPDAHGTVELDGPYDVGLSMPCLGTASDPTWRGDRTRVELAGR
                     TPEGPVAVRARAEDGHLRLDAWGPGAGWMVARLDGLAARHDDPRLLQFGHPVLDETNR
                     RRAGMRHAPTGLVVDGLLARVLGQRVLATEAARSWNAMCRELGGPAPGPLDLLLPPDP
                     ERLAEMPTWWFHERGVERGRARTIVAVCRQARRLAEVVDLPLPQAYGRMRAVPGLGPW
                     TVNGVARVALGDPDAIVVGDYWISHAVCSFFTGRARGSDEEMLGLVDRWVGQRGRVER
                     LIHLSGHRVQRFGPGVPTPRIAHL"
     gene            8684..9127
                     /locus_tag="VLR27_15850"
     CDS             8684..9127
                     /locus_tag="VLR27_15850"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSP04985.1"
                     /translation="MADEATASATTHVDATAEEVFEFIRRPVNHAEISGDGSVKGTRH
                     GAEVLSAEGDQFGMSMKMYGLPYRITNTVVEYEDGRRIAWCHPGKHRWRWEVEPVATG
                     GCTVTETFDMSTSPLKPVLKLMGFPGKHQPNVEKSVVNVAKHFAG"
     gene            9192..10265
                     /locus_tag="VLR27_15855"
     CDS             9192..10265
                     /locus_tag="VLR27_15855"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HSP04986.1"
                     /translation="MSRIGVPTLVVGASAAVLALGLGGHGLLVPAGPASTSVRVVGAV
                     VAVAATVGLVGLDGPLRAGRTLLLVAVTGGLTVLATLPASPVTIDAGDVPEASAPAGR
                     DPQRTILLEQLRPGEAQDGTLVLPPGATVGPDGEDVVVQAPGGETTVLAPGQRVAQPD
                     GSVVVVGPGGRMEVPEPRPGDAELVPDRLAAGVEAVLALLLGGFALLAFAPPLVRVAE
                     RSLTRVAPDPAPAPARPPSRSVEQGLADVLRAMLADPDPRTAVIGAYARLLAALDEAG
                     FPRRAEEAPHEHLWRSLGPLGVRRPPVHRLAELFVRARFTPHPVTDEHRRAAIAALAD
                     AVADLRLPDRDVDELVSTVVGPR"
     gene            10262..10708
                     /locus_tag="VLR27_15860"
     CDS             10262..10708
                     /locus_tag="VLR27_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04987.1"
                     /translation="MNRRLLSASIAAAALTGAGAVVFPQHAASLVLLAAGTVVVVAAV
                     FLLVLAGPLVTTERPVSALDVVPGGGAAPLDPHGLRDARRDLAVRHAPGSLPLPVHDR
                     LAAAGVLPASPPPPPGTTVDRDAAAALVHRVLDGPDAPTGPDGGAP"
     gene            10705..11694
                     /locus_tag="VLR27_15865"
     CDS             10705..11694
                     /locus_tag="VLR27_15865"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSP04988.1"
                     /translation="MTTIDDDHPATEATPTTATVALAGRIVDEVERAVIGKRDVVDLV
                     LAAVLAGGHVLLEDVPGVAKTLLARSLATACGLGFARVQFTPDLLPSDITGTLVLDLA
                     TRTPTFRPGPVFTDVLLADEVNRAPAKSQAALLEAMQEGQVTVDGTARDLGRPFAVIA
                     TQNPIEAEGTYPLPEAQLDRFLVRVALGYPSAEDELAILGGRLARRQPEVDIRPVVSR
                     QTFVSMQAAVEAVHVDDSLVAYALALVRATRDEPDIAIGASPRGSLALVTLARAWALV
                     RGRDYVGPDDVRALAVPTLSHRITLDDDAWVRGTLADDVVRRVIDRVPAPTWS"
     gene            11691..12995
                     /locus_tag="VLR27_15870"
     CDS             11691..12995
                     /locus_tag="VLR27_15870"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSP04989.1"
                     /translation="MTTVRRAEAPVVQSQVAADRRFGPYVVLAALAAVLAVVTGGVAY
                     AATGVPVGLLLAWRSRHGRAPGVAVRVHEPPARVLEGGRWELVVDLVWSGPAEVDVVL
                     DGVGGHVVLGLPGAAVRADGGTRLRFALEAQRWGRHRLGRLTVRARRPGGMVVHDLVV
                     EVPGVVRVLPAASRLDSLLHPARPRAAAGAHAAPTRGPGTDFADLRPYVPGDRLRDVS
                     WAASARSDDPWVVVHHPERTGTVVLLLDGFVEAGLPAGALDRAARVVWSVARHHLVNG
                     DRVGLVAAGPTPRWLPPVAGRRARWAVLDALLRAEAVTAGRGPRRTPASARRQDEAVP
                     ADAFVVGVSPLQSDAFVAAVAHHRRLGRPTAVVGVEVADLLPPADDEVERAARRLWRL
                     EADLRRAKLSRAGVRTLLVTDDVTPAIRALSSLPHRAVRQSA"
     gene            12992..13438
                     /locus_tag="VLR27_15875"
     CDS             12992..13438
                     /locus_tag="VLR27_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP04990.1"
                     /translation="MMVLRLARAGGLACALAVAIVAGATLGGWWWLAAALAGVGALTG
                     RTGSLVHAVATLVLAGSASVEDVTWLVPLLVLGIVAGVETAALPERATRVRTDVPVAP
                     ALAVPVAAAGTAVAVLALATVAPDFAVPTALAATLAAVALLTALRP"
     gene            13602..14513
                     /locus_tag="VLR27_15880"
     CDS             13602..14513
                     /locus_tag="VLR27_15880"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HSP04991.1"
                     /translation="MLDVVTALAWDQHGAFSTAQAEGRGVARSSLTRRCRDGVLLRRA
                     QGVYALAVAPRTARQDLMVHLLAAGDGAFATDDSALALWCPELVLPAKPVIVVPRSCG
                     YRTRQAIVRRGLDTDRVNPGVVDGIPTVGVARALLDASIGRSPDEVLSRIDACRRHGS
                     LATGALLEVLDQHARRGRPGIVTFRQAVRGLRREVTDSEFERLVIRDLVAAGLPAPRL
                     HHVVRLPGEGPIELDLDWPGLLLDVELDGADHKERARRMARDRKRDRLLQAAGYLVAR
                     YTWDDYVADRAGMIAEIVELRAAARRS"
     gene            complement(14566..17163)
                     /gene="valS"
                     /locus_tag="VLR27_15885"
     CDS             complement(14566..17163)
                     /gene="valS"
                     /locus_tag="VLR27_15885"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HSP04992.1"
                     /translation="MSSIPDKPTIDGLEAKWRAIWDEQGTYRFDRSKTRDEVYSIDTP
                     PPTVSGSLHIGHVMSYTHTDTLARYQRMRGREVFYPMGWDDNGLNVERRVQMLTATLC
                     DPTLPYDPDFTPPQPDAKGKLPKDPVRISRPNFVELCEQVVQEMEDEYHRLWATVGLS
                     VDWEQTYTTIGPVASRISQKAFLRLLDEGVAYRSGAPTLWDVDMQTAVAQAELEDREV
                     PGAYHKLAFGLPGGGGPDGDVLWIDTTRPELLPACVAMVAHPDDERYQPLFGRTAVTP
                     LFGVEVPILAHELAQPDKGTGIAMICTFGDTTDVIWWRELGLPIRPVIRHDGRFEREL
                     TWSGSPEHEDDVWVSTDPVAANEAYAELAGKTVFTAQKNIVALLSESGAMEGEPRPIT
                     HPVKFWENGKKALEIVTSNQWFIRYPPAEEIIERAKGLAFHPEYMRVRFEDWAKGLQG
                     DWNITRQRFFGVPFPIWYPIDDEGLVDYLSPIVATEDMLPVDPTTMVPPGFEEAQRNQ
                     PGGFAADPDVMDTWNTSSMSPQIASKWEEDPDLFERVYPMDLRPQAHDIIRTWLFSTV
                     VKAHELFDDLPFRHAAISGFVVDPDRKKLSKSKDNAEVGPFQLLEMFGADAVRYWACG
                     GGPGRDVANDQGQMKIGRRLAIKLLNASKFALLQGEPSPGATVTEPLDRAMLLGLADL
                     VDEATRVFDAYDYARALERTETYFWTFTDDYLELVKGRAYGDDAAADSAKVALRLALE
                     TLLKLFAPFLAYTAEEVWSWWQEGSIHRSPWPVADDLRTAAGGPDPDAALALTVAGEV
                     LSLLRKAKSDAKVSRRTEITTATITDTPERLAAFERVRADVADAGKVQQVQTAEGDTL
                     TVEATLADS"
     gene            complement(17212..18378)
                     /locus_tag="VLR27_15890"
     CDS             complement(17212..18378)
                     /locus_tag="VLR27_15890"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSP04993.1"
                     /translation="MDEEVQQGDRDALLIGDAEREATIAALRDAVGDGRVTLDEFADR
                     AGATYAATTVADLRAVVADLGGPDLPAPAPRGVQAPPPAPPGSQRVVAVLGAAERKGR
                     WTVGSELSAVAVMGSVELDLYDAALTAEVTTIRAFALMGAVEIVVPEGVPVQVEGFVL
                     MGALEDRTRSHRGQQPMLRIIGHGMWGAVEVRHRKARSKGARRPDRAPTPLLEQPASA
                     GDTFTLLFTDIVGSTVLAERLGDQRWAEVLREHNTLVRDQISHHGGDEIKNQGDGFMV
                     AFRSARRALLAAIAIQRALEAYRRDHPEHPLHVRIGVHSGEVVADEGDLHGRNVVLAS
                     RISDLAGRDEVLASALTKQLADAGGDLCFGAGRHVALKGLNGEWLVHPVEWSTS"
     gene            18497..19831
                     /gene="cysS"
                     /locus_tag="VLR27_15895"
     CDS             18497..19831
                     /gene="cysS"
                     /locus_tag="VLR27_15895"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HSP04994.1"
                     /translation="MLHLFDTARGAVVPFEPSTPGKASIYACGPTVYGPPHLGHGRNQ
                     VSYDLLRRYLAWSGYEVTFVSNITDIDDKIIERARNEERPWQDITQRCESVWFQGMDR
                     LGILRPDHTPHATAYVDEMVELIQRLVDRDVAYATSDGVYLDVSQIDGYGLLAHGELD
                     QLRSGARVEANEEKRSPLDFALWKLAKPGEPSWPAPFGDGRPGWHTECVVMSLDILGE
                     GFDIHAGGNDLQFPHHENERAQAVAEGKRFANLWFHHGMVEIDGEKMSKSLGNVSNLL
                     DLMDAYDPRAFRLLVLRAHYRSPIDVSDDSMRDAEAAIARLDGFARRFAGKVAGVDAD
                     PGSIERFRAAMDDDLKTPTAVATLFDAVNRANKDGDLAAAAAAFEIAGVLGLELRGEA
                     ATVPEHVVAQARARDEARAAKDWAAADQIRDELTAAGWVVEDTADGTTVRPG"
     gene            complement(19852..21264)
                     /locus_tag="VLR27_15900"
     CDS             complement(19852..21264)
                     /locus_tag="VLR27_15900"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP04995.1"
                     /translation="MKRTTDTTTTGDQDLVAAAGRGDQAAFDELHRRHAPLAWRLALA
                     VTGRPEDAATAVVEGTGATFTAVRAGRFQANSHGTALATASRNAALDLRRGQDAIAAP
                     VAADDADPFLAGAFGALPERWRSVLWLRDAEDLDAEAVAPIVELTPEAVDQLAVRARR
                     GLRERYLKAQAATATDRGCTRAIARLGALEDGTLNDADRTTLEGHLAECEACTGRRAG
                     IAAIPTALTALATAPPADLHERTRAAHASALATTSTTGLSPRTEKILAGASAFAAAVG
                     VLGATLFGAGGGEEPVASPLAPLVSDINAPRPIDLSALTLPITTPDPVTTTARRSLSP
                     AVRSAALGTDPATGGTAGGELAAPAPDAGGPASDPGTPAAEAPSSPLPGGDDVPISVD
                     VGEDTSVTVGPVTADLTPEPDEDPITIDEPEALSPVVEPVEEVVNTVVEPVTQAAQPV
                     LEAAEPVTDGVGQVLGGLGR"
     gene            21523..>21663
                     /locus_tag="VLR27_15905"
     CDS             21523..>21663
                     /locus_tag="VLR27_15905"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP04996.1"
                     /translation="MAEFSLQLNDDQIQIRDWIHEFAKDVLRPAGQEWDDKEEFPWPI
                     VQE"
BASE COUNT         2799 a         7786 c         8146 g         2932 t
ORIGIN      
        1 ccggtcggtc agccacgcgg cggcggcgtc ggcgtcgtcg gcggtggggc agcgggccat
       61 gacctcccgg ccgcccttgc gctcgaggcg gtcgacggcg gtgaggatga cggcggggtc
      121 cacgacgaag aggtgctcgg gccacggcac caccgggtcc ttggccccct tgccggtgtt
      181 gcagccccgg tgggccaggc gctcctgacc gtcggccccc ttcttcttcg ccttggcctt
      241 ggtcgtgcgc aggtcgatgc tcgggccccg ggcgtcgttc accgacatgt cggggtccac
      301 cggctcgtcg cagagccagc accgccagcc gtcctgctcg ccgacctcgt cgaggcggct
      361 catggggacg ggacggggga gaggaccacg gcggcaccgt aaggcagtgg gcggcgattc
      421 gtcgacgatt cgtcagctcg agaccgtgcc gccgataccg tcggcccatg gcctccgaat
      481 cccgcaccga gaccgactcc atgggcggca tcgacgtcgc cgccgaccgc tactggggtg
      541 cccagaccca gcgcagcatc ggcaacttcc cgatcggacg ggaccgcttc gtctggggtc
      601 gcccgatcat cgaggcgttc ggcctggtga agtgggccgg cgcccgggcc aacacccagc
      661 tgggcgagct cgacgaggag atctccgagc tggtccagcg ggccgccgac gaggtcatcg
      721 ccggcgacct cgacgaccac ttcccgctgg tcgtgtggca gacgggctcg ggcacgcagt
      781 cgaacatgaa cgcgaacgag gtcatctcca accgggccat cgagatggcc ggcggcgaga
      841 tcggctccaa gtcgcccgtg cacccgaacg accacgtcaa ccggggccag tcgagcaacg
      901 acacgttccc caccgccatg cacatcgcca tcgtcctcga gctgcaccgc cagctgtacc
      961 ccggggtcat ggccctgcgc gacgcgctgc gggacaaggc ggtggagttc gccgacgtgg
     1021 tcaaggtcgg ccgcacgcac ctgcaggacg ccaccccgat caccatgggc caggagatcg
     1081 gcggctgggt ccaccagatc gactgggccc tcgagcaggt gcggcgcagc gaggagggcc
     1141 tgctcgagct ggccatcggc ggcaccgccg tcggcaccgg gctgaacgcc cacccccgct
     1201 tcggggctgt cgcggccgcc gagctcgccg agcgcaccgg ccacgagttc cgggtcgccg
     1261 gcaaccggtt cgccgccctg tcggcccacg acgcgctcgt cgccaccagc ggcgcgctgc
     1321 gcaccctcgc cggggcgctc atgaagatcg ccaacgacat ccggtgggag gcgtccggtc
     1381 cccgcaacgg catcggcgag ctgcgcatcc ccgagaacga gccgggctcg agcatcatgc
     1441 cgggcaaggt caacccgacc cagtccgagg cgctgacgat ggtggccgtc caggtcttcg
     1501 gcaacgacgc cgccgtggcg ttcggcgggt cccagggcaa cttccagctc aacgtgttca
     1561 agccggtgat ggcccacaac gtgctggaga gcatcgccct cctcggcgac gccagcgcct
     1621 ccttcgagga gcactgcgca cgagggctgg aacccgacca cgaccgcatc cgccacaacc
     1681 tcgagcagaa cctgatggtc gtgacggcgc tcaaccccca catcggctac gacaagggcg
     1741 cacagatcgc caagaaggcc cacgccgagg gcacgacgct gcgggaggcc gccatcggct
     1801 ccggccacct caccgccgag cagttcgacg agtgggtcga cccgatggcc atgacccgag
     1861 ccgactgagg agctacagca gctcgtactc cggggcgtcc ttcttcgccg cctcgggcgg
     1921 cagcccgcac tgcaggaccg ggtagcggcg acgcacgccg acgcgggcgg tgcgctcgcc
     1981 ggcgccggtg gccccgtccc agcggagggt gacggtggct gacgtcccgt ccgcatcgac
     2041 atcgacgtcg gcctcgaggt ggtcgacgtc gagggcagcc cacccgtgct cggacaggcc
     2101 gacggcctcc acgacctggg cccaggggtc gaggccgacg tggccgcggt agtggtcccg
     2161 ggcgtcgatg gggtggagcg tgcggtcgac gatgccggcg atggcctcga cgtccaggaa
     2221 cgcccagacg cgaccgtcgg gcagggagag cgcggtgggg gcgaaccggt ggccgcccag
     2281 gtggctcgtc cgccggatcc ggacctcggg cagggcggct cgggcctcga ccgcgaggcg
     2341 ggtgcccgat ccgccgcagc accggtcgcg ggcgccgtgc ccgcagacga ggatctcgtc
     2401 gggcgcgccc tcgccgggga cgtcggggtc cccgccggat gcgacgacaa cgagcgcgtc
     2461 gggtacgtcg actgccggta cgagccagtc cgtgccggtg aaccgggcgc cgtcggcggt
     2521 ccagcgggtg accagcacgg tgccgtcggt gcgggcgacc tcggggacga tggtctgcat
     2581 ccgggtgccg gggtcgagct cgaggtcggc gatccagggg tggtcggcga cgtcggccgg
     2641 ccagggcagc ggcacctcga cggtgaccag ccggtcgatc tccacggccg agccgatcgg
     2701 gtccacaccc tggtccaggg tccacggggc gcagcgcagc tcgaggggtc cgtcgacgga
     2761 gggggtcacc tccgcagtat gcccagggcg gacggatgag ccgcgggccc agggcgcacc
     2821 cggccggggg cgggcagtcc ggccggatgc gccctcgacc cgcggccgag ggtggtgcgc
     2881 tggttcaggc ggtgcggcgg cggccgccgc ggacgctgcg cacgacgagg aggagcacga
     2941 ccgagccgat gatcgagccg aagatgccgc ccatctggac ggcgccgtcg tcgaggtcgg
     3001 cgccgaagat caggtagccg aggaagccgc cgacgaagga gccggcgacg ccgagggccc
     3061 acgtgccgac gatgcccatg gggtcccgac cgggcacgag caggcgagcg atggctccga
     3121 agatgatccc gaagaagaag agcacgagca ggaaccagag catggcggtc ccttcccccc
     3181 accgtgggtg gccagacccg gacgagaccg gagtcaccgc aacgtcagca ctgccagttc
     3241 gaggaggggg tcaggtggcg aagcgggcga cgatctcgag gacgtcgtcg ccgtagctgt
     3301 cgagcttgga gggcccgacg cccttgatgc ggctgagggc ccggaggtcg gcggggcggg
     3361 ccttggcgat gtccttcagc ggtgcgtcgc cgaagatgat gaaggccgga ccgccctgtc
     3421 gcttggccac ctcgctgcgc cagcgccgca gctcctcgaa caggacgagg tcctcgccct
     3481 cgaggtcggg ctcggaggtc ttggctgcct tcgctgcctt cgccgagcct gcggcccggc
     3541 tgccggtcgc cgctccggtg cgagcggtcc ggcgaggtgc ctgcttgctg gcggtgccgt
     3601 cgagctcggc gaggaacggg ctgggtcggt cgtcgtcggc gagcacggtg accgcctcga
     3661 tgccacgggt gatggcgacg tggaagacgc gtcgctcctc ctcgacgtcc tcggcgagcc
     3721 ggtgggggaa gatcccggcg tcggcgccga acacgatgac gtggggccac tcccggccct
     3781 tcacccggtg gaccgtggac agggtgacgc cggcggcagc ctcggcggtg ggatcgccgt
     3841 cggacgctcg gggaccgtcg agggcacccc gcagccaggc ctcgaactcg tccgggtcgg
     3901 gctgcaggtc ggcgacctgg aggagcgcgt cgaggtcgtc gaggtgggac gacccctcgg
     3961 cgcccggccg gccgtcgagc agctccatgg cgccgccgag gccgatgtcg tcgcggatgg
     4021 cctggaggat ctcacgggtg gtggcgcccc gggccagatc gccgaggcgg tcgatgtcgg
     4081 cggcgaggtc gaggagcttg tcggcgacgc gcgcgtcgtc gatgcgctcg gccgccttgg
     4141 cgacctcgtg gccggagcgg caccggccca gccacttgtc gacccagttg gggaagcctc
     4201 ggctcgggcg gcggcggatc tcggtgaggt cggtcgatgc gatggacccc ggatcggtgg
     4261 cgacccgcag ccaggccagc gccgcccgga ggccgacccg ctggaggacg tcgcggcgca
     4321 gcacggatgc gatggggatg ccggcaccgt gcagggccac gtgcggggcc agcaggagcg
     4381 agttggtgcg ggtgagcacg gccacctgcc ccgggtcgcc cgtggcctcg acggcggcgc
     4441 ggacggcctc gaccacgcgg gtggcgccct cctgcggcgg gtgctgggtg acggtgaggg
     4501 catcaccagg accggcggga cgggccgccg tgatggtctt ggccacccgc tcgtcgttgt
     4561 agcccagcag gtggcgcgcc gcgtcgacca cggcgggcgg gcagcggtag ttgacctgga
     4621 gcgcgtgctc gtgggcgccg gggaacaggt cggcgaaccg gagcaggaac tgcgggctcg
     4681 caccggcgtg gccgtagatc acctggtcgt cgtcgcccac gccgaagacg tcgtaggtgg
     4741 gggcggccag cagtcgcagc agcagcacgt gggccggcgt caggtcctgg aactcgtcga
     4801 cgagcaggtg gcggtgccgg ttctgctccc gccggcggaa ctcgccgtcg gacaggagca
     4861 gctcgacggc gtggatgatc tgctcgtcga agtcgagcac cgagtcccgc cgcagtccct
     4921 cgcggtaggg gccgacggca gccgcgaggc cgggcgcgtc ggcctcgagc tcgacctcgt
     4981 cggggtcgcg caggccgagg cggatgaccg agagcccctc caggtacggc gccatcgggt
     5041 cggtgttgag gcggtgctgg agcttgggga ggtggggttc gaggcggcgg cgcacctcgc
     5101 gctcgtcgag catctgtgga cggcgtccgg cgtggcggcc gatgagctcg tagcccaggg
     5161 cgttcagcgt ctggatgcgg gcgccgaggc cggcggtgcg cccggccatc tcctcctggg
     5221 ccttgcggtt gtaggcgacg gcgagcagca ggtcgcgctc gtggccgcgg tcggcgagga
     5281 ggtggcgcag gcgctcggtg aggacccggg tcttgcccga accggccggg gcgatgatgc
     5341 gggccggccc gccgcggtgg gcgaccgccg ccagctgatc gggtgccagc tcggcgctgg
     5401 gtgcgaccca ggccggcacg gggcggaggt ggccggcgtc gacggactcg gcccggacga
     5461 gcacggcggg tgcgagggtg gcggggtcga cgtcgtcggg cggacccccg tcgatccacg
     5521 cgaccgtgcc gtcgggcagc aggacgtcgc cctcgacggg gtcgtgcccg gccggcagct
     5581 cgtcgacggc cgtgccgtcg atgcgggccg ccttgcgtcc ccaccaccac acgacctcgt
     5641 cgccgcgggc gtcgtagctg ttgtgccaga cgaggaagtg caggcggtcg agccacggct
     5701 cgaacgtcgg cgacagctcc cacggcacgt cggtgtggga cacggggcga cggaaccgga
     5761 ccgggtccac ccgcaggtcg accacgacgc gctcgcgacc cgcccaggcg gcgtggagcc
     5821 gctcgacgac ggcccccggc tgggcgagga cgtcctcgtc gaccaccacg acgggcaccc
     5881 cggcccacgg cgccggcggc gaggcgccgt cggccaccac gacgccgcgg gcgagctgat
     5941 cgggtccggc gtgcatcggg tcgatcgtag ggacggccac cgacagccac ggtaggcgcg
     6001 tccggcggcc ttggcgaacg gacgttcgaa tctctagttc ttggtagatg aatgggacct
     6061 tggtccccgt cctgggggta cttgtcctcg ccgccgccac ccctccgacg cctcagcatg
     6121 gtctccaaca cccctttccc gaggagctcg agatgagcat cgaactgcaa gaacaaggac
     6181 acgagaagac ggcgaagcgt gacggctggc tggtgctggt cgccacgttc agcgccttgg
     6241 cgatgatcgt catgctcttc gccgccggcg tcgccgtgcg cggcggagac gacgacggag
     6301 gcggtggtgc cccggttgcg accgagacca tcgacgtcac cctcggcgac ctcttcgtcg
     6361 agccgtcgag cctgaccatg ccggccggcg ccaccctgac gctgaacgtc accaacgacg
     6421 gcgccatgcc ccacgacctc aaggtcggtg gcacggatgg cacggcgatg ctcgatcccg
     6481 gcgcatccga gacgatcacg atcggcccgt tcgatgcctc cgtcgaggct tggtgcacgg
     6541 tgcccggtca ccgcgagggc ggcatgaaca tgatgatcac cgtcgagggc gaggccggcg
     6601 gcgagaccgc cgtggctgct gccgagggcg atgctgccga ggtcggaggt gccgccctcg
     6661 gtggtgccga gatcgacttc aacgccgccc ccgagggtga ctggcagccg ttcgaccccg
     6721 cgctggcgcc tgcccccggc ggcaccgagc acgaggtcac ctggagcatg accgaggagg
     6781 tcatggagat cgctcccggt gtgacccagg aggtctggac cttcgacggc caggtgcccg
     6841 gcccgaccct gcggggcaag gtcggcgaca tcttcaccgt cacgatcgtg aacgacggcg
     6901 agatgggcca ctcgctcgac ttccacgcca gcaaggtggc ctggaacgac gagatgcgca
     6961 ccatccagcc gggcgaggag ctggtgtacc agttcgaggc caagcacgcc ggggcgttca
     7021 tgtaccactg cggcacggcc ccgacgctgc accacatcgg caacggcatg cacggcgcga
     7081 tcatcatcga cccgccggac ctggcgccgg tcgaccacga gttcgtcttc gtccagcacg
     7141 agtactacac gggcggcgag ggtgagcctg gcgacctggg caagatgcag cgtgacgagt
     7201 gggacgccgt ggtcttcaac ggctacgtca accagtacgt ccacgccccg atcgacggca
     7261 tcgaggtcgg cgagcgggtg cgggcctggg tcgtcgacaa cggcccctcg gagaacagct
     7321 cctggcacgt cgtcggcacg gtcttcgaca ccctctacaa ggagggcgcc taccagctgc
     7381 gacccggtga cgggaaccag ggcggcgccc aggcgctgga cctccagccg gcccagggcg
     7441 gcttcgtcga gttcaccttc gacgaggagg gcgcctaccc gttcgtgacc cacaagttct
     7501 cgaacgccgg caagggcgcc atgggcctgt tcgtggccgg cgatcccgat ctcgagggca
     7561 tcggcggtca ctgagcctcg ggaaggcgct ccggccccgg caccgcttcg gcggtgaccg
     7621 gggccgtggc gcgtccgggg tcagaggtcg gcctggatga tcacgctccg ctgctcatcg
     7681 actggcggct ccgccgccgt agggcaccgt gacatgggtc gcctcgcctt cggctcggct
     7741 cctacaaatg cgcgatgcgc ggcgtcggca cgccggggcc gaagcgctgc acgcggtggc
     7801 cgctcaggtg gatcagccgc tcgacccggc cgcgctggcc gacccaccgg tcgaccaggc
     7861 cgagcatctc ctcgtcggat ccgcgggccc ggccggtgaa gaacgagcac acggcgtggc
     7921 tgatccagta gtcgccgacg acgatggcgt cggggtcgcc gagggccacc cgggcgacgc
     7981 cgttcaccgt ccacggaccc aggccgggaa cggctcgcat gcggccgtag gcctggggga
     8041 ggggcaggtc gaccacctcg gcgaggcggc gggcctggcg gcacaccgcg acgatcgtcc
     8101 gggcccggcc ccgctcgacg ccgcgctcgt ggaaccacca ggtgggcatc tcggcgaggc
     8161 gctccgggtc tggcgggagg agcaggtcca gggggccggg cgcgggcccg cccagctctc
     8221 ggcacatggc gttccacgag cgggcggcct cggtggccag cacccgctgc ccgagcacgc
     8281 gggccagcag cccgtcgacg accaggcccg tcggggcgtg gcgcatcccg gcgcgacggc
     8341 ggttggtctc gtccagcacc gggtgaccga actgcagcag ccgcggatcg tcgtggcgag
     8401 cggccaggcc gtcgagccgg gccaccatcc acccggcacc cgggccccag gcgtcgagcc
     8461 ggaggtgccc gtcctcggct cgggcgcgga ccgcgaccgg accctcgggc gtgcgtccgg
     8521 cgagctccac ccgggtgcgg tcgccgcgcc aggtcgggtc cgaggcggtc ccgaggcagg
     8581 gcatggacag gccgacgtcg taggggccgt cgagctcgac ggtgccgtgg gcgtccgggc
     8641 gcatgcggac gcgacggtag cggtgcgcgg tagaacgtct gccatggccg acgaagcgac
     8701 cgcatccgcc accacccacg tcgacgcgac ggccgaggag gtgttcgagt tcatccgccg
     8761 gccggtcaac cacgccgaga tcagcggcga cgggagcgtc aagggcacgc ggcacggcgc
     8821 cgaggtgctc tccgccgagg gcgaccagtt cggcatgtcg atgaagatgt acggcctccc
     8881 gtaccggatc accaacacgg tggtggagta cgaggacggc cgacgcatcg cctggtgcca
     8941 cccgggcaag caccgctggc gctgggaggt cgagccggtg gccaccggtg gctgcaccgt
     9001 caccgagacc ttcgacatgt ccacctcgcc gctcaagccg gtgctcaagc tcatgggctt
     9061 ccccggcaag caccagccga acgtggagaa gagcgtcgtg aacgtggcca agcacttcgc
     9121 cggctgacgc ctgatcgccc ggcccgggca tccccgaggc cgcaggtcga tctcggtacc
     9181 gttcggtcgg tatgtccaga atcggcgtcc cgaccctggt cgtcggcgcg agcgccgcgg
     9241 tcctggccct cggcctcggc gggcacggcc tgctcgtccc ggccggcccg gcatcgacgt
     9301 cggtccgggt ggtgggtgcc gtggtcgcgg tcgccgccac cgtcggcttg gtcggcctcg
     9361 acgggccgct gcgggccggc cgcacgctgc tgctcgtcgc ggtgacgggc gggctgaccg
     9421 tgctcgccac gctccccgcc tcaccggtca cgatcgatgc cggcgatgtc ccggaggcga
     9481 gcgcaccggc gggccgggat ccacagcgca ccatcctgct cgagcagctc cgccccggcg
     9541 aggcccagga cggcacgctc gtcctgccac cgggagccac ggtggggccg gacggtgagg
     9601 acgtggtcgt gcaggcgccg gggggtgaga ccaccgtgct cgcgccgggc cagcgggtcg
     9661 cccagccgga cgggagcgtg gtcgtcgtcg gcccgggcgg gcggatggag gtgcccgagc
     9721 cccgacccgg cgacgccgag ctcgtgcccg accgtctcgc cgcgggggtg gaggccgtgc
     9781 tcgccctgct cctcggcggg ttcgccctcc tggccttcgc cccgccgctc gtgcgggtcg
     9841 ccgagcgctc cctgacccgg gtcgcccccg atccggcgcc ggcgcccgcg cgtccaccct
     9901 cgcgctcggt cgagcagggc ctggccgacg tgctgcgggc catgctcgcc gaccctgatc
     9961 cgcgcacggc ggtcatcggg gcgtacgccc gcctgctggc ggcgctggac gaggccgggt
    10021 tcccccgccg ggccgaggag gcgccccacg agcacctgtg gcgcagcctg ggaccgctcg
    10081 gcgtccggcg cccgccggtc caccgcctcg ccgagctgtt cgtccgagcc cgcttcacgc
    10141 cgcaccccgt caccgacgag caccgccggg cagccatcgc cgcgctggcc gatgccgtgg
    10201 ccgacctccg cctgccggac cgcgacgtcg acgagctggt gagcaccgtg gtcggaccgc
    10261 gatgaaccgc cggctcctct ccgcgtcgat cgccgccgcc gccctcacgg gtgccggtgc
    10321 cgtggtcttc ccccagcatg cggccagcct ggtgctgctc gcagcgggca cggtcgtggt
    10381 ggtcgccgcc gtgttcctgc tcgtgctggc cggtccgctg gtgacgaccg agcgcccggt
    10441 gtccgcgctc gacgtggtgc ccggcggcgg tgcggcaccg ctcgatccgc acggcctgcg
    10501 cgacgcccgc cgcgacctcg ccgtccgcca cgcgccgggc tcgctgcccc tgccggtgca
    10561 cgaccgcctc gcggccgcag gcgtcctgcc ggcgtcgccg ccaccgccgc cggggaccac
    10621 ggtcgaccgg gacgccgcgg cggcgctcgt gcaccgcgtg ctcgacggac ccgacgcgcc
    10681 caccggtccc gacggaggag ccccatgacc acgatcgacg acgaccaccc cgccaccgag
    10741 gcgacgccca ccaccgccac tgtcgccctc gccgggcgca tcgtggacga ggtcgagcga
    10801 gcggtcatcg gcaagcgcga cgtcgtcgac ctggtgctgg ccgcggtgct ggccggcggt
    10861 cacgtgctgc tcgaggacgt ccccggcgtg gccaagacgc tgctcgcccg cagcctggcg
    10921 acggcgtgcg ggctcgggtt cgcccgggtc cagttcacgc cggacctgct gccgtccgac
    10981 atcactggca cgctggtgct ggacctggcc acccgcacgc ccaccttccg acccggcccg
    11041 gtcttcaccg acgtgctcct cgccgacgag gtgaaccggg cgccggccaa gtcgcaggcc
    11101 gcgctgctcg aggccatgca ggagggccag gtgacggtcg acggcaccgc ccgcgacctg
    11161 gggcgcccgt tcgcggtcat cgccacccag aaccccatcg aggccgaggg cacctacccg
    11221 ctgcccgagg cccagctcga tcgcttcctc gtccgggtcg ccctcggcta cccgagcgcc
    11281 gaggacgagc tggccatcct cggtggccgc ctggcccgtc gccagcccga ggtcgacatc
    11341 cgtccggtcg tgtcccgcca gacgttcgtg tccatgcagg ccgccgtcga ggcggtccac
    11401 gtcgacgact cgctcgtggc ctacgcgctc gccctcgtgc gggccacccg cgacgagccc
    11461 gacatcgcca tcggggccag cccccgcggg tcgctggcgc tggtgacgct ggctcgggcg
    11521 tgggcgctgg tccggggccg ggactacgtg gggcccgacg acgtgcgcgc cctcgccgtg
    11581 ccgacgctga gccaccgcat caccctcgac gacgacgcct gggtccgcgg gaccctggcc
    11641 gacgacgtcg tgcgccgggt gatcgaccgg gtgcccgccc ccacctggtc gtgaccacgg
    11701 tgcgccgggc cgaagcgccg gtggtccagt cgcaggtggc ggccgaccgc cggttcggcc
    11761 cctatgtggt cctcgccgcc ctggccgcgg tgctggccgt cgtgaccggt ggggtggcgt
    11821 acgccgcgac cggggtcccg gtcgggttgc tcctcgcctg gcgcagccgg cacggccggg
    11881 cgccgggcgt ggccgtgcgg gtccacgagc ctccggcacg ggtgctggag ggtggccggt
    11941 gggagctggt ggtggacctg gtgtggtccg gtccggccga ggtcgacgtc gtgctcgacg
    12001 gcgtcggcgg ccacgtggtg ctcggtctcc cgggcgccgc ggtgcgggcc gacggcggca
    12061 cccggctgcg gttcgcgctg gaggcccagc gctggggccg gcaccgcctc gggcggctca
    12121 ccgtccgtgc tcgccggccc ggcgggatgg tcgtgcacga cctggtggtg gaggtgcccg
    12181 gcgtggtccg cgtgctgccc gcggccagcc gcctcgattc tctcctccac ccggctcgac
    12241 cccgggcggc cgccggcgcc cacgccgccc ccacccgcgg tcccggcacc gacttcgccg
    12301 acctgcgccc ctacgtcccc ggcgatcggc tccgcgacgt gtcgtgggcc gcctcggccc
    12361 gcagcgacga cccctgggtc gtcgtgcacc acccggagcg gacgggcacg gtcgtgctcc
    12421 tgctcgacgg gttcgtcgag gccggcctcc ctgccggcgc gctggaccgg gcggcccggg
    12481 tcgtgtggtc ggttgctcgc caccacctcg tcaacggcga ccgggtcggc ctcgtcgccg
    12541 ccgggccgac gcctcgctgg ctgccaccgg tggcggggcg ccgggcccgg tgggccgtgc
    12601 tggacgcgct cctccgggcc gaggccgtca cggcggggcg gggcccccgg cggaccccgg
    12661 cgagcgcccg gcgtcaggac gaggcggtgc cggccgacgc gttcgtcgtc ggcgtcagcc
    12721 ccctgcagtc cgacgcgttc gtcgccgccg tggcccacca ccgtcgcctg ggccggccga
    12781 ccgcggtggt cggcgtcgag gtcgccgacc tgctgccgcc ggccgacgac gaggtcgagc
    12841 gcgccgcccg ccggctgtgg cgcctcgagg ccgacctgcg ccgggcgaag ctctcccggg
    12901 ccggcgtgcg cacgctgctg gtcaccgacg acgtcacgcc ggcgatccga gccctctcct
    12961 cgctgcccca ccgggccgtg cggcagtcgg catgatggtg ctccggctcg cccgggccgg
    13021 cggcctcgcc tgcgcgctgg ccgtcgcgat cgtcgccggc gccaccctcg gcggctggtg
    13081 gtggctggcc gccgccctcg ccggcgtcgg cgccctcacc gggcgcaccg gcagcctcgt
    13141 ccacgccgtg gccaccctgg tcctcgccgg cagcgcatcg gtcgaggacg tcacctggct
    13201 ggtgccgctc ctggtgctcg gcatcgtcgc cggcgtcgag accgccgccc tgcccgagcg
    13261 cgccacccgc gtccgcacgg acgtccccgt cgcccccgcc ctcgccgtcc cggtcgccgc
    13321 cgccggcacg gccgtcgccg tcctcgccct cgcgacggtc gcccccgact tcgcggtccc
    13381 caccgccctg gccgcgaccc tcgccgccgt cgccctcctc accgccctcc gaccctgacc
    13441 ccccacccgt tctggcagtg ctgctgtagc gctgacgttc tcgggctgtg gcgttgcgtc
    13501 agcgccacgc cctgcgccga gaagcggctc ggccgtgggt gtcggtcgtc gtcggtacgg
    13561 tgatgaccac ttcccccgag cgatcgagag ggggaaggcg cgtgctcgac gtcgtcacgg
    13621 cactggcgtg ggaccaacac ggtgcgttca gcaccgcaca ggcagagggc agaggtgtgg
    13681 cccgcagctc gctgacgcgc cgctgccggg acggcgtgct gctgcgacgc gcccagggcg
    13741 tctacgcgct ggccgtcgcc ccccggaccg cgcgacagga cctgatggtc cacctcctcg
    13801 ccgccggcga cggtgccttc gccaccgatg attcggccct cgcgctctgg tgccccgagc
    13861 tggtcctgcc tgcgaagccg gtcatcgtcg tgccgaggag ctgcggctac cgcacccggc
    13921 aggccatcgt ccgccgtggt ctcgacacgg atcgcgtcaa cccgggcgtc gtcgacggca
    13981 tccccaccgt cggcgtcgcc cgagccctgc tcgacgcgtc gatcgggagg tcccccgacg
    14041 aggtgctgtc ccgcatcgat gcctgccgcc gacacggttc cctcgcgacc ggcgccctgc
    14101 tcgaggtgct cgatcaacat gcacggcgag gacggccggg catcgtcacg ttccggcagg
    14161 ccgtgcgagg gctacggcgg gaggtcaccg actcggagtt cgaacggctg gtcatccgtg
    14221 acctggtcgc tgctggactg ccggcacccc ggctccacca cgtcgtccga cttccaggtg
    14281 aggggccgat cgagctcgac ctggactggc ccggattgct gctcgacgtc gagctggacg
    14341 gcgccgacca caaggagcgg gctcggcgca tggcccggga tcggaagcgg gatcgactgc
    14401 tgcaggccgc cgggtacctg gtggctcgct acacctggga cgactacgtc gccgaccgcg
    14461 ccgggatgat cgccgagatc gtcgagctgc gcgccgcagc acggcgaagt tgaccttctc
    14521 gggtcgtggc gttgcgccag cgcaacgccg tgcgccgaga acggctcagc tgtcggcgag
    14581 cgtggcctcg acggtgaggg tgtcgccttc ggcggtctgc acctgctgga ccttgccggc
    14641 gtcggcgacg tcggcccgga cccgttcgaa cgcggcgagg cgctcggggg tgtcggtgat
    14701 ggtggcggtg gtgatctcgg tgcgcctgga gaccttggcg tcggacttgg ccttgcgcag
    14761 cagggacagg acctcgccgg cgacggtgag ggccagggcg gcgtcggggt cggggccgcc
    14821 ggcggccgtg cggaggtcgt cggccaccgg ccagggggag cggtggatgg agccctcctg
    14881 ccaccacgac cacacctctt cggccgtgta ggcgaggaac ggggcgaaca gcttgagcag
    14941 cgtctcgagc gcgaggcgca gggcgacctt ggccgagtcg gccgcggcgt cgtcgccgta
    15001 ggcccggccc ttgaccagct cgaggtagtc gtcggtgaag gtccagaagt aggtctcggt
    15061 ccgctcgagc gcccgggcgt agtcgtaggc gtcgaacacg cgggtggcct cgtcgaccag
    15121 gtcggcgagg ccgagcagca tggcccgatc gagcggctcg gtgacggtgg cgcccggcga
    15181 gggctcgccc tggagcaggg cgaacttcga cgcgttcagc agcttgatgg cgaggcgccg
    15241 gccgatcttc atctgcccct ggtcgttggc gacgtcgcgc cccggaccgc cgccgcaggc
    15301 ccagtagcgc acggcgtcgg cgccgaacat ctcgagcagc tggaacgggc cgacctcggc
    15361 gttgtccttg gacttcgaca gcttcttgcg gtccgggtcg acgacgaagc cggagatcgc
    15421 ggcgtggcgg aacggcaggt cgtcgaacag ctcgtgggcc ttgacgacgg tggagaacag
    15481 ccaggtgcgg atgatgtcgt gggcctgggg tcgcaggtcc atcgggtaca cccgctcgaa
    15541 caggtcgggg tcctcctccc acttcgacgc gatctgcggc gacatcgacg acgtgttcca
    15601 cgtgtccatg acgtcggggt cggcggcgaa gccacccggc tggttgcgct gggcctcctc
    15661 gaagccgggc gggaccatgg tggtggggtc gaccgggagc atgtcctcgg tggcgacgat
    15721 gggggagagg tagtcgacca ggccctcgtc gtcgatcggg taccagatcg ggaacggcac
    15781 gccgaagaag cgctgccggg tgatgttcca gtcgccctgc aggcccttgg cccagtcctc
    15841 gaagcgcacc cgcatgtact cggggtggaa ggcgaggccc ttggcccgct cgatgatctc
    15901 ctcggccggc gggtagcgga tgaaccactg gttggaggtg acgatctcca gtgccttctt
    15961 gccgttctcc cagaacttca ccgggtgggt gatggggcgg ggctcgccct ccatcgctcc
    16021 cgactccgac agcagcgcca cgatgttctt ctgggcggtg aagacggtct tgccggccag
    16081 ctcggcgtag gcctcgttgg cggcgaccgg gtcggtggac acccagacgt cgtcctcgtg
    16141 ctcgggcgag ccggaccagg tcagctcccg ctcgaagcgg ccgtcgtggc ggatgaccgg
    16201 ccggatcggc agcccgagct cgcgccacca gatcacgtcg gtggtgtcac cgaaggtgca
    16261 gatcatggcg atgccggtgc ccttgtcggg ctgggccagc tcgtgggcga ggatggggac
    16321 ctcgacgccg aacagcgggg tgaccgccgt gcgcccgaac agcggctggt agcgctcgtc
    16381 gtcggggtgg gcgaccatgg cgacgcaggc ggggagcagc tcggggcggg tggtgtcgat
    16441 ccacagcacg tcgccgtcgg ggccgccgcc accgggcagg ccgaacgcga gcttgtggta
    16501 ggcgccgggg acctcgcggt cctcgagctc ggcctgggcg accgcggtct gcatgtcgac
    16561 gtcccacagc gtcggcgcac ccgaccggta ggccacaccc tcgtcgagca ggcgcaggaa
    16621 cgccttctgg gagatccggc tcgcgaccgg gccgatggtg gtgtaggtct gctcccagtc
    16681 gacggacagg ccgacggtgg cccacaggcg gtggtactcg tcctccatct cctggacgac
    16741 ctgctcgcac agctcgacga agttgggtcg cgagatgcgc accgggtcct tcgggagctt
    16801 gcccttggcg tcgggctggg gcggcgtgaa gtcggggtcg tagggcagcg tcgggtcgca
    16861 cagcgtcgcg gtgagcatct ggacgcgccg ctcgacgttc aggccgttgt cgtcccagcc
    16921 catggggtag aagacctcac gcccccgcat gcgctggtac cgggccagcg tgtcggtgtg
    16981 cgtgtaggac atcacgtggc cgatgtgcag cgaacccgac accgtcggcg gcggcgtgtc
    17041 gatggagtac acctcgtcgc gggtcttgga ccggtcgaag cggtaggtgc cctgctcgtc
    17101 ccagatcgcc cgccacttgg cctcgagccc gtcgatggtc ggcttgtcgg ggatgctgct
    17161 cacgggcgct gagggtaccg gtggcctctc gatcctcccg aaacacaaac gtcagctcgt
    17221 ggaccactcc accgggtgca cgagccactc cccgttgagg cccttgaggg cgacatggcg
    17281 tcccgcaccg aagcacaggt cgccgccggc gtcggccagc tgcttggtga gggccgaggc
    17341 gaggacctcg tcgcgacccg ccaggtcgct gatccgggat gcgagcacga cgttgcggcc
    17401 gtggaggtcg ccctcgtcgg cgacgacctc gccggagtgc acgccgatgc ggacgtggag
    17461 cgggtgctcg gggtggtctc ggcggtaggc ctcgagggcg cgctggatcg cgatggcggc
    17521 gagcagggcg cggcgcgcgg aacggaacgc caccatgaac ccgtcgccct ggttcttgat
    17581 ctcgtctcct ccgtggtggc tgatctggtc ccggacgagg gtgttgtgct cgcgcagcac
    17641 ctcggcccag cgctggtcgc cgaggcgctc ggcgaggacg gtcgacccga cgatgtcggt
    17701 gaacaggagg gtgaaggtgt ccccggccga cgctggctgc tccagcagcg gcgtgggagc
    17761 ccgatcgggg cgccgagcgc ccttgctgcg ggccttgcgg tggcgcacct cgaccgcgcc
    17821 ccacatgccg tggccgatga tccgcagcat gggttgctgc ccgcggtggg acctggtgcg
    17881 gtcctcgagc gcccccatga gcacgaagcc ctccacctgc accgggaccc cttcgggcac
    17941 cacgatctcg acggcgccca tgagcgcgaa ggcgcggatg gtcgtcacct cggcggtcag
    18001 ggcggcgtcg tacaggtcga gctcgacgct gcccatcacc gccaccgccg acagctcgga
    18061 gccgaccgtc cagcgaccct tgcgctcggc agcacccagc accgcgacga cccgctggga
    18121 tccgggcggc gccggcggcg gggcctggac accacgcggt gccggcgcgg gcaggtcggg
    18181 gcctccgaga tccgccacca ccgctcgcag gtcggcgacg gtggtggcgg cgtaggtggc
    18241 gccggcccga tcggcgaact cgtccagcgt cacccggccg tcgccgacgg cgtcgcgcag
    18301 cgcggcgatg gtcgcctccc gctcggcgtc gccgatcagc agcgcgtcgc ggtctccctg
    18361 ctgcacctct tcgtccaccg gtccccccga acgtcgtggt cgagcggccc atcggccgca
    18421 ggtgcagtct ggcccaaccg gccacgcgcc cgcttctccc tgggtcggac ggcggggcgt
    18481 cggtaccctc ggcgccgtgc tgcacctgtt cgacaccgcc cggggcgccg tcgtcccctt
    18541 cgagccctcc acgccgggga aggcctcgat ctacgcgtgc gggccgacgg tctacgggcc
    18601 gccgcacctc gggcacggcc gcaaccaggt ctcctacgac ctgctccgcc ggtacctggc
    18661 ctggtcgggc tacgaggtca ccttcgtgtc gaacatcacc gacatcgacg acaagatcat
    18721 cgagcgcgcc cggaacgagg agcgtccctg gcaggacatc acccagcgct gcgagtccgt
    18781 ctggttccag ggcatggacc gcctcggcat cctgcgcccc gaccacaccc cgcacgccac
    18841 cgcctacgtc gacgagatgg tcgagctgat ccagcgcctg gtcgaccgcg acgtcgccta
    18901 cgccacgtcc gacggcgtct acctcgacgt gtcccagatc gatggctacg gcctgctcgc
    18961 ccacggggag ctcgaccagc tgcgcagcgg cgcgcgcgtc gaggccaacg aggagaagcg
    19021 gtcaccgctc gacttcgcgc tctggaagct ggccaagccc ggcgagccgt cgtggccggc
    19081 ccccttcggc gacggccggc ccggctggca caccgagtgc gtcgtgatga gcctggacat
    19141 cctcggcgag ggcttcgaca tccacgccgg cggcaacgac ctgcagttcc cccaccacga
    19201 gaacgaacgg gcccaggccg tggccgaggg gaagcgcttc gccaacctgt ggttccacca
    19261 cgggatggtc gagatcgacg gcgagaagat gtccaagtcg ctcggcaacg tgtcgaacct
    19321 gctcgacctc atggacgcat acgacccccg ggccttccgc ctgctggtgc tgcgcgccca
    19381 ctaccgctcg ccgatcgacg tgagcgacga ctcgatgcgc gacgcagagg cggcgatcgc
    19441 ccgcctcgac gggttcgccc gtcgcttcgc cggcaaggtc gccggtgtcg acgccgatcc
    19501 ggggagcatc gaacgcttcc gcgcggccat ggacgacgac ctgaagaccc cgaccgcggt
    19561 ggccacgctc ttcgacgcgg tgaaccgggc gaacaaggac ggcgacctgg ccgcagccgc
    19621 tgcggccttc gagatcgccg gcgtgctcgg gctcgagctg cggggtgagg cggcgaccgt
    19681 ccccgagcac gtggtcgcgc aggcgcgggc ccgcgacgag gcccgagcgg ccaaggactg
    19741 ggccgcggcc gatcagatcc gcgatgagct cacggccgcc ggatgggtcg tggaggacac
    19801 cgccgacggc accacggtcc gcccgggctg acgcccgggg agaccgtcgg atcagcggcc
    19861 gaggccgccg agcacctggc cgacgccgtc ggtcaccggc tcggccgcct ccagcacggg
    19921 ctgggcggcc tgcgtcaccg gctcgacgac cgtgttcacg acctcctcga ccggctcgac
    19981 caccggcgac agtgcctcgg gctcgtcgat ggtgatcggg tcctcgtcgg gctccggggt
    20041 gaggtccgcc gtgaccgggc cgaccgtgac gctcgtgtcc tcgccgacgt cgacggagat
    20101 cggcacgtcg tcgccgccgg ggagcggtga ggagggcgct tcggcggccg gcgtgccggg
    20161 gtcggaggca ggaccgcccg catcgggggc cggtgcggcg agctcgccgc cggcggtgcc
    20221 gcccgtggcc gggtccgtgc cgagcgccgc ggaccggacg gccggggaca gcgaccggcg
    20281 tgcggtggtc gtgacggggt ccggcgtggt gatcggcagc gtgagggccg agaggtcgat
    20341 ggggcggggc gcgttgatgt cggagacgag gggtgcgagg ggggacgcga ccggctcctc
    20401 gccgccaccg gcaccgaaca gggtggcgcc gagcacgccg accgccgcag cgaaggcgct
    20461 ggcgccggcg aggatcttct cggtgcgggg ggagaggccg gtggtggagg tggtggcgag
    20521 tgcggaggca tgcgcggcgc gggtccgctc gtgcaggtcg gcgggcggcg cggtggcgag
    20581 tgcggtgagg gcggtgggga tcgccgcgat gccggcccgg cgcccggtgc aggcctcgca
    20641 ctcggccagg tgcccttcga gggtggtccg gtcggcgtcg ttgagggtgc cgtcctcgag
    20701 ggcgccgagc cgggcgatgg cgcgggtgca gccgcggtcg gtggcggtgg cggcctgggc
    20761 cttcaggtag cgctcgcgca ggccacggcg ggcccggacg gccagctggt cgacggcctc
    20821 gggggtgagc tccacgatgg gggcgacggc ttcggcgtcg aggtcctcgg cgtcgcgcag
    20881 ccacaggacg ctgcgccagc gctcgggcag cgcgccgaac gcaccggcga ggaaggggtc
    20941 ggcgtcgtcg gcggccacgg gggcggcgat ggcgtcctgg ccacggcgca gatcgagggc
    21001 ggcgttgcgg ctggcggtgg cgagcgcggt gccgtggctg ttggcctgga agcgcccggc
    21061 ccgcacggcc gtgaacgtgg cgccggtgcc ctccacgacc gcggtggctg cgtcctcggg
    21121 ccggccggtc acggccaggg cgaggcgcca agcgaggggt gcgtggcgcc ggtgcagctc
    21181 gtcgaaggcg gcctggtcgc cgcgcccggc ggcagcgacg aggtcctggt cgccggtggt
    21241 ggtggtgtca gtggtccgct tcatgtcgcc cccagatcag tggttcggat cgaggcttcg
    21301 ccccctccca cggtctgtcc ttcccatctt cgcggttcgt catcgaaatg cagcgcggaa
    21361 ggccgtcgaa accggacaga tcggtagggt tcgggctccg tcacccggcc gatccacggg
    21421 caccggctgg ccgccgaggg ttactcatcg gtaacttcga cggggacgtg gcgctgcgcc
    21481 cgcggtgcct gcccgatccg ctcctgcccc cggaggaacc ccatggccga gttctcgctc
    21541 cagctcaacg acgaccagat ccagatcagg gactggatcc acgagttcgc caaggacgtg
    21601 ctgcgtccgg cgggccagga gtgggacgac aaggaggagt tcccctggcc gatcgtgcag
    21661 gag
//
LOCUS       DATISU010000400        13434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_314307, whole genome shotgun sequence.
ACCESSION   DATISU010000400 DATISU010000000
VERSION     DATISU010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 13434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13434
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314307"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="VLR27_15910"
     CDS             complement(<1..1289)
                     /locus_tag="VLR27_15910"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSP04997.1"
                     /translation="MPALSIADALHGQRIAITGGTGFLGTALIERLLRRAPGCELVLL
                     IRPSQRRSVEQRARREIFKNDAFDLLRSQLDGGESFDAMIERRVQVIGGDVGTDGLGL
                     DEAGREILATCDTVIHSAATVSFDSPLDLAVEVNLLGPSRIATVLHELCADRDPQAPK
                     PHLVAVSTCYVAGNRKGAAPEEPVDENAFWFGVDWRSEVAASRRSRADFEAESRRPDM
                     LARFRKEARRELGAAGTPLLAAKTEQRRSAWVSDQLVEAGRTRAASLGWPDAYAYTKA
                     LGEKALFEHARAPVAGGGDLPFSIVRPAIIESSLAEPSPGWIRGFRMAEPVIISYARG
                     LLKEFPGVPEGIIDVIPVDLVCGAICAVAADGPARNDDGSLAPPRIVQSASGSANPLR
                     YRRLVDNVRSWFTEHPVYDSDGQPIVVPEWNFPGRGRV"
     gene            1380..3161
                     /locus_tag="VLR27_15915"
     CDS             1380..3161
                     /locus_tag="VLR27_15915"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSP04998.1"
                     /translation="MGDTGGMGGMAEGSTLLGALPVDVRQAVVARLEPRSLHGGETLV
                     EQGDEADGLYLVHAGRLQATMRTGDGDTVLGEIGRGEVVGEAALLTEQRRTATIVALR
                     DTELWHLPLAAFDELVAEHPSFLRPIAAQVVQRMLTAGSRQVASQPVTTVAIVPMHGT
                     ELASDATAALADALAAVVGRGLVVDSGDRPDDRSDAGWIQSLEVDNDLVVFRCDADAD
                     DDADDATRRFLRQADAVVLVADAAQGPAVTPCESLLAEHRERVDVPVELVLVHPATCV
                     EARGTRRWLDGRRLRRHHHVRAGDEAHAARVARLVTDRGIGVVLSGGGARSMAEIGVI
                     AAMVELGIPIDAVGGTSAGALVAGAFARGWSVQEVRDVLHQGMVAQGSPIDPTVPFTS
                     LAAGRRMTERLQAAAGEVDIEDLWLPFYCVSTNLSRNRPEIHRRGRGWRAVRASMSIP
                     GIFPPVAEGGDVLVDGGLVDNLPVAEMRRAHEGITVVAVDVGVHRGMNAGDLPDSTVV
                     HGWRLLRDRIHPGRRSPKIAGIIAILTRLTELGGGSVATDLGDVLVRPDVDRFPMLDF
                     SRFDDLIAVGEREGRSALSDWWRSREP"
     gene            3151..3816
                     /locus_tag="VLR27_15920"
     CDS             3151..3816
                     /locus_tag="VLR27_15920"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSP04999.1"
                     /translation="MNPDRILAWLARFARLLTAPFVRYDIRGGRCARELDCGVIAMNH
                     RSMFDIVVGLIALHHFGHCPRLLVDRTYVESRWTAPFARALGAIPVDRIKGGGTSVEE
                     ALAALRNGTSIVVMPEGRLHRDADDPTTTGPTRTGVSRLAVGSGLPVVPVGTTGTTAV
                     LPYGARVPRFNPFRRKTVICHVADDAIWLTGDDHRANTDEVMAAIRALLVLPSQREER
                     PDR"
     gene            3794..4381
                     /locus_tag="VLR27_15925"
     CDS             3794..4381
                     /locus_tag="VLR27_15925"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP05000.1"
                     /translation="MRNDRTADRILETALRSFGTTGIDATSLDALARSLGVTKQAILY
                     WYPSKEALLDAVIDFSAVELQRRFARAIETGEGFDRIEAVVHAAFRLAARHPAMLGLM
                     REVNRIGPPTSTRLTESVTPLLTAAAAWLGAEMDAGRLRRHDPKLLVLMAYSSVTGLA
                     TEVEVLRALGEEPTLASLVRRRDQLVDLLRDALVP"
     gene            4427..5353
                     /locus_tag="VLR27_15930"
     CDS             4427..5353
                     /locus_tag="VLR27_15930"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HSP05001.1"
                     /translation="MRLHRILAVPFVLALVAAGCTDADDDTSSTAQDSTDSSDSAGAD
                     DGSDLDVATDGASAGEGSGGSRVADVEQVDLEAVPIAAVDRKIIYTGTVSSVVEDVAA
                     AVREVRDAATVEGGYVFAQDLGPGQGRVVLKVPVDRFDAVFDAVVAIGEVEAQHIEAE
                     DVSERFVDLESRQATLATSIDRIRGFLAQTTNVDEISRLESELTNREAEHDVIAGQLR
                     VLRDRTSYGTISVDLRLQDPDAALVTEEAESGPPGFGEGLSAGWDAVTTAGAAAATVV
                     GFVLPFAPVLVLAGGLSWWWRRRTRADVTATA"
     gene            complement(5337..5765)
                     /locus_tag="VLR27_15935"
     CDS             complement(5337..5765)
                     /locus_tag="VLR27_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05002.1"
                     /translation="MDTRARTLAALIGAATGVAVGVAAASSLERHLVRGESMVPALRP
                     RDTVISVRAGSILGRFLRPRRSRIAVIALPWEHDLIGVKRLVAEPGEPWEDGDDRVAA
                     GPGWVAVGDKRELSTDSRHHGRVPADAIRGIVVGRVKPSQ"
     gene            5793..6206
                     /gene="sodN"
                     /locus_tag="VLR27_15940"
     CDS             5793..6206
                     /gene="sodN"
                     /locus_tag="VLR27_15940"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HSP05003.1"
                     /translation="MSIVSRLLSVADLDDAATADAHCDLMCGVYNPAQARIEAESVLE
                     TMKKYNASDDDYFKTRCIIIKEQRAQLAKEHLWVLWTDYFKPPHVEQFPQLHDLFWRA
                     TKAAGDAKKSNDEADAQKLLDLIDEISEIFAETKK"
     gene            complement(6207..7268)
                     /locus_tag="VLR27_15945"
     CDS             complement(6207..7268)
                     /locus_tag="VLR27_15945"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HSP05004.1"
                     /translation="MALPDRIRLVMLFGGRSAEHEVSCTTATHVLRALDPGRYEPVPV
                     GIARDGSWLQTEGPLPDQLVVAGTPVEPVTALQPQPAAPGGPDIPVVVLPLLHGPFGE
                     DGTVQGLLEMADVPYVGSGVLSSSVSMDKVAAKELLSAHDIPQGAWMSARRHEIDDEL
                     LERAADRLGFPMFVKPANMGSSIGISKVHEVEHLAAAVAEASRFDEWILFEENIVGRE
                     LECGVLGNLTPRASVVGEVRPGADFYDYDDKYEDGAELLIPAPISAPMSDELREIALA
                     TFAALRCEGMARVDFFWEEDGRGPLVNEVNTIPGFTPISQYPKLWEASGLPYGRLLDE
                     LVALALERHERRSSFETSR"
     gene            7309..8619
                     /gene="murF"
                     /locus_tag="VLR27_15950"
     CDS             7309..8619
                     /gene="murF"
                     /locus_tag="VLR27_15950"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HSP05005.1"
                     /translation="MELSIDEVAAAVRGRVVGPPVTVSRVAIDSRTVGEGQLFVPLVA
                     ERDGHDFVPSAVSAGASAYLTDRGPLDDGATAVHVDDTMEALTRLGAVARDRLPDAVV
                     GITGSVGKTSVKDLLAGVLATTYRTTASEKSFNNEIGVPLTLVNAPADTEVLVLEMGA
                     RGIGHIRDLCEVARPSVAVVTMVAEVHTSEYENGLDDVARAKGELVESIPVDGHAVLN
                     GSDARVRRMAGLTDARVLTYGVSGDVVAQRVDLDDHLRPRFRLSSPWGTVDVQLAVHG
                     VHQVGNALAAAAAALVQGVPLDGVAEGLASADLSPWRMELRHTDAGAVVVNDSYNSNP
                     TALEAALHSLAAIDADRHVAVLGVMAELGHHSDQRHAEMGELAEELGIEVITVDCPAY
                     GVGTDVPTPEAAVDALGSLGPDDAVLVKASRSAGLERVAALLLG"
     gene            complement(8628..9392)
                     /locus_tag="VLR27_15955"
     CDS             complement(8628..9392)
                     /locus_tag="VLR27_15955"
                     /inference="COORDINATES: protein motif:HMM:NF016365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HSP05006.1"
                     /translation="MRGLGTVVNVVTVLAGTGVGVLLHGRMPERVRSTVLDGIGLIVL
                     GVGIASFLETDNAVFPVVAIVAGGVLGELLRIEDRLEGLGERLRRLVEGPASEPVIAS
                     YVERDPDELEVDRHVPTGRSFVDGFLIASLTFGIGAMMIVGSLQDGISGDSELLIVKA
                     TLDGIVSVPFAAVYGWGVGFSALSIAVLQGAITLLGATLGDSLLDDRMVAELEATGGV
                     MILGIGLRLLDLKKVKVGSFLPALVIAPVLVAVFAS"
     gene            complement(9456..10679)
                     /locus_tag="VLR27_15960"
     CDS             complement(9456..10679)
                     /locus_tag="VLR27_15960"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSP05007.1"
                     /translation="MPYIADRVVHDADAHIMETPTWLRDHADPAIRDRIEPLPLTSGN
                     ELRQTGDVEEQLADLTAAFDRLAAKHASEEYRAEEAHEIMLRKNFAATGSFIPEDRVR
                     ALDLIGVQSQLVFNTFHNSRLFAWEHSGDLEMAYGTARAHNRGMAEFCSVDERLLSTM
                     YVPLADFDRAAAMAGEALHLGAAALLIPSGCPATHSPSHRSLDPVWAQAQEAGIPIVF
                     HVGGTGELIDPMYFQNGLPIPPDFHGGDENFRCVDYMGIPGPPAQTLATMLFDGVFER
                     FPDLRAGVIEQGAIWVPGWMRQMESAFDAFHRHEERLQQLSMRPSEYVSRQMRFTPYP
                     TEDVGWIIEQAGPETVLFSTDFPHVEGGRRPVERFETSLDAAGVSDDDRDRFYRRNFV
                     DLMGNGLPAVLAAAS"
     gene            complement(10701..11972)
                     /locus_tag="VLR27_15965"
     CDS             complement(10701..11972)
                     /locus_tag="VLR27_15965"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP05008.1"
                     /translation="MTAPADATEEDGEEGDEEGPQWGGQSMAALRQQTFRRVYTGAFF
                     SNIGTWMQNVVLGALAYDISGGSSSFVGIVLFAQLGPMLFFSIIGGMLADSFDRRHLL
                     MIVSATQAALAVVLAVVVAGEDPNRWAIVGVVFLIGLGQSVFGPTYSAVLPDLVGPGN
                     LAGAISLNSTQMNGSRVIGPAIGGLLFARYGAEVVFSLNAATYLFVIGALASIRLPDV
                     ETAGAGESRIERLALGFRIARNRFPIGRSLVTMIVFSFLCLPFVGQMPALAERNLGIA
                     ATSSAYGWLYACFGLGAMLGALSVGTTFSEVPKEDVTRWGMIAFGIALGVFAWLRTPV
                     PAYLVIIVVGFFYFATVTALSTVLQMHLAGHERGRVMALWMMAFGGTVPLGNLVAGPI
                     IDATSVTTVVFPGAFVALFLAWWADLRPKQA"
     gene            12066..13280
                     /gene="dinB"
                     /locus_tag="VLR27_15970"
     CDS             12066..13280
                     /gene="dinB"
                     /locus_tag="VLR27_15970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HSP05009.1"
                     /translation="MSSAGILHADLDCFYAAVEQRDDPRLRGRPVIVGGGVVLTASYE
                     ARVHGVRTAMPVPQARSLCPSAIVVPSRMSAYTEASRAVFAIFRETTPLVEGLSVDEA
                     FLDVNGLRRLVGEPIEIARELRRRVRDEVGLPISVGVARTKFLAKVASAVSKPDGLLV
                     VEPDGELDFLHPLDVRRLWGVGPVTAGKLDERGLRTVGDVAGVPESALVAMLGPAAGR
                     HLHALARNVDPRSVTPGTRRSSIGAQQALSRAGGRRPWSRAELDAALLALVERVTERL
                     RGGRRVGRTVTLRLRHADLSRETRSRTLPVATDRTDLILGAARDLLDARWSHLREAGC
                     SLIGVTVSELAPADAVQLSLDLDGHDRRTLDHTLDAVRDRFGGSAVTRASLLGEGRGA
                     RVPILPDPVPDP"
BASE COUNT         1931 a         4765 c         4923 g         1815 t
ORIGIN      
        1 acgcggccgc ggccggggaa gttccactcg ggcacgacga tgggctggcc gtcgctgtcg
       61 tacacggggt gctcggtgaa ccaggaccgc acgttgtcga ccaggcgccg gtatcgcagc
      121 gggttggccg agcccgacgc cgactgcacg atgcggggcg gggccaggga gccgtcgtcg
      181 ttgcgggccg gaccgtcggc cgcgaccgcg cagatggcac cgcacacgag gtcgaccggg
      241 atgacgtcga tgatcccctc ggggacgccg gggaactcct tcagcaggcc gcgggcgtag
      301 gagatgatga ccggctcggc catgcgaaag ccccggatcc atccgggact gggctcggcg
      361 agcgacgact cgatgatggc cggacgtacg atcgagaacg gcaggtcgcc gcccccggcc
      421 accggtgcgc gagcgtgctc gaacagcgcc ttctcgccga gggccttggt gtaggcgtag
      481 gcgtcgggcc agccgagcga ggcggcgcgg gtccggccgg cctcgacgag ctggtcgctg
      541 acccaggcgg agcggcgctg ctcggtcttg gcggccagga ggggcgtgcc ggcggcgccg
      601 agctcgcggc gggcctcctt gcggaaccgg gcgagcatgt cgggccggcg ggactcggct
      661 tcgaagtcgg cccgcgaccg ccgggaggcg gcgacctcgc tgcgccagtc gaccccgaac
      721 cagaacgcgt tctcgtcgac cggctcctcc ggcgcggcgc ccttgcggtt gccggcgacg
      781 tagcaggtgg acacggccac caggtgcggc ttcggtgcct gcgggtcacg gtcggcgcag
      841 agctcgtgga gcacggtggc gatgcgggac gggccgagca ggttgacctc gacggcgagg
      901 tcgaggggtg agtcgaagct caccgtggcg gccgagtgga tgacggtgtc gcaggtggcg
      961 aggatctcgc ggccggcctc gtcgaggccg aggccgtcgg tgccgacgtc gccgccgatg
     1021 acttgcacac gccgctcgat catcgcgtcg aagctctcgc ccccgtcgag ctgcgaccgg
     1081 aggagatcga acgcgtcgtt cttgaagatc tcgcggcggg cccgttgctc gacgctgcga
     1141 cgctggctcg ggcggatgag caggaccagc tcgcatcccg gcgcccgacg caggaggcgc
     1201 tcgatgaggg cggtcccgag gaaccccgtc ccgccggtga tggcgatgcg ctgaccgtgg
     1261 agcgcgtcgg cgatggagag ggccggcatc gggcccgttc taccaccttt ccttaccagt
     1321 tggtaggtag aggtgccggc cggtgggggt gccgactccc gtccttggac gtcgacgacg
     1381 tgggggacac ggggggcatg gggggcatgg ccgagggcag caccctcctc ggggcgctgc
     1441 cggtcgatgt gcgacaggcc gtggtggcgc ggctcgagcc gcgctccctg cacggtgggg
     1501 agacgttggt ggagcagggc gacgaggccg acggcctcta cctggtccac gccggtcggc
     1561 tgcaggccac catgcggaca ggagacggag acacggtcct cggtgagatc ggccgcggcg
     1621 aggtcgtggg cgaggcggcg ttgctcaccg agcagcgtcg aacggccacg atcgtggcgc
     1681 tgcgcgacac cgagctctgg cacctgccgc tcgccgcgtt cgacgagctg gtggccgagc
     1741 acccttcgtt cctgcgaccg atcgccgcgc aggtggtgca gcggatgctc accgcaggca
     1801 gccggcaggt cgcctcgcag ccggtcacca ccgtggcgat cgtccccatg cacggcacgg
     1861 agctcgcttc ggacgcgacg gcggcgctgg cggacgcgct ggctgccgtc gtgggacggg
     1921 ggctggtggt cgacagcggc gaccgccccg acgaccgcag cgacgccggc tggatccagt
     1981 cgctggaggt ggacaacgac ctggtggtct tccgctgcga cgccgatgcc gatgacgacg
     2041 ccgacgatgc gacccgacgg ttcctccgcc aagccgacgc ggtggtgctg gtcgccgacg
     2101 ccgcgcaggg gccggccgtc acgccgtgcg agtccctgct cgccgagcac cgcgaacgcg
     2161 tcgacgtgcc ggtcgagctc gtgctggtgc acccggcgac ctgcgtggag gcgcggggga
     2221 cccgccggtg gctcgacggg cgacggctcc gtcgacacca ccacgtgcgc gccggcgacg
     2281 aggcgcacgc ggcccgggtc gcccggctgg tcaccgatcg cggcatcggg gtggtcctct
     2341 cgggtggcgg ggcgcgcagc atggccgaga tcggcgtcat cgcggcgatg gtcgagctgg
     2401 ggatccccat cgacgccgtc ggtggcacga gcgccggtgc gctcgtcgcg ggggcgttcg
     2461 cacgcgggtg gtcggtccag gaggtgcgcg acgtcctcca ccaggggatg gtggcccagg
     2521 gcagcccgat cgacccgacg gtgccgttca cctcgctggc ggccgggcgg cgcatgaccg
     2581 agcggctcca ggccgcggcc ggtgaggtcg acatcgagga cctctggctg cccttctact
     2641 gcgtctccac gaacctgtcc cgcaaccgcc ccgagatcca ccggcgcggt cggggctggc
     2701 gggcggtgcg ggccagcatg tcgatcccgg gcatcttccc gccggtggcc gagggtgggg
     2761 acgtgctcgt ggacggcggt ctggtcgaca acctccccgt ggccgagatg cgccgggcgc
     2821 acgagggcat cacggtggtg gcggtcgacg tgggtgtgca ccgaggcatg aacgccggcg
     2881 atctcccgga ctcgacggtc gtgcacggct ggcgcctgct ccgcgaccgg atccaccccg
     2941 gacgtcgctc gccgaagatc gccgggatca tcgcgatcct cacccgcctc accgagctcg
     3001 ggggcggaag cgtcgccacc gacctcggcg acgtcctcgt gcgccccgac gtcgaccggt
     3061 tcccgatgct ggacttctcc cgcttcgacg acctgatcgc cgtcggcgag cgcgagggcc
     3121 ggtctgcact ctccgattgg tggaggtctc gtgaaccctg atcgcatcct ggcctggctg
     3181 gcccgcttcg cccggctcct caccgcgccg ttcgtgcgct acgacatccg cggtgggcga
     3241 tgtgcccgcg agctggactg cggggtcatc gcgatgaacc accgcagcat gttcgacatc
     3301 gtggtcggcc tcatcgcgct gcaccacttc ggccactgcc cccggctgct ggtcgaccgg
     3361 acctacgtgg agtcgcgctg gaccgccccg ttcgcccggg cgctgggcgc catccccgtc
     3421 gaccggatca agggcggggg cacgtccgtc gaggaagcgc tcgccgccct gcgcaacggg
     3481 acatccatcg tggtcatgcc cgaaggtcgc ctccaccggg atgccgacga cccgaccacg
     3541 accggaccga cgcggacggg cgtgtcccgg ctggccgtcg gttccgggct gcccgtcgtg
     3601 ccggtgggga cgacggggac caccgccgtg ctgccctacg gcgcccgcgt cccgcggttc
     3661 aacccgttcc ggcgcaagac cgtgatctgc cacgtggccg acgacgcgat ctggctcacc
     3721 ggcgacgacc accgcgccaa caccgacgag gtcatggccg ccatccgtgc actgctggtc
     3781 ctaccgtcgc agcgtgagga acgaccggac cgctgaccgc atcctggaga ccgcgctgcg
     3841 cagcttcggc acgaccggca tcgacgcgac ctcgctggat gcgctcgccc ggagcctggg
     3901 ggtcaccaag caggcgatcc tctactggta cccgtcgaag gaggcgctcc tcgacgcggt
     3961 gatcgacttc agcgccgtgg agctccagcg gcgcttcgcc cgggccatcg agacggggga
     4021 gggcttcgac cgcatcgagg cggtcgtgca cgcggcgttc cggctggcgg cgcggcaccc
     4081 ggcgatgctc ggcctgatgc gcgaggtgaa ccgcatcggc ccgccgacgt cgacccggct
     4141 caccgagtcg gtcaccccgc tgctcaccgc cgccgcggcc tggttgggtg ccgagatgga
     4201 tgccggccgg ctgcgccgcc acgaccccaa gctgctggtg ctgatggcgt actcctcggt
     4261 caccgggctg gcaaccgagg tcgaggtgct ccgggcgctg ggggaggagc ccacgctggc
     4321 gtcgctcgtg cgccggcgcg accagctggt cgacctgctg cgggacgccc tcgtgccctg
     4381 acgcggagcc gggaaccgag gacgcccgca cgagcgtcgg atctgcatgc gcctccaccg
     4441 gatcctcgcc gtcccgttcg tcctggccct cgtcgcggcc ggctgcaccg acgccgacga
     4501 cgacacctcg tccaccgccc aggacagcac cgacagctca gacagcgccg gtgcggatga
     4561 cgggagcgat ctcgatgtcg cgactgacgg cgcatcggct ggtgaggggt cgggtggaag
     4621 ccgggtggcc gacgtcgaac aggtcgacct ggaggcggtg ccgatcgccg cggtggaccg
     4681 caagatcatc tacacgggca cggtcagctc cgtggtcgag gacgtggccg ccgccgtgcg
     4741 ggaggtccgg gacgctgcga cggtggaggg cggctacgtg ttcgcccagg acctcggccc
     4801 cgggcagggc cgggtggtgc tcaaggtgcc cgtcgaccgc ttcgatgccg tgttcgacgc
     4861 ggtcgtggcc atcggcgagg tcgaggccca gcacatcgag gccgaggacg tgagcgagcg
     4921 cttcgtcgat ctcgagagcc gccaggccac cctcgccacc agcatcgacc gcatccgggg
     4981 cttcctcgcc cagaccacga acgtcgacga gatcagccgg ctcgagtccg agctgaccaa
     5041 ccgcgaggcc gagcacgacg tcatcgccgg ccagctgcgg gtcctgcggg accgcacgag
     5101 ctacggcacg atcagcgtcg acctgcgctt gcaggacccc gatgccgcgc tcgtcaccga
     5161 ggaggccgag agcggcccgc ccgggttcgg cgaggggctg tcggccgggt gggatgcggt
     5221 caccacggcc ggggccgcgg cggcgacggt ggtcgggttc gtcctgccct tcgctcccgt
     5281 cctggtgctg gccggcgggc tcagctggtg gtggcggcgt cgcacccgcg ccgacgtcac
     5341 tgcgacggct tgacccgccc gacgacgatg ccccggatcg cgtcggcggg cacccggccg
     5401 tggtggcggc tgtcggtgga gagctcgcgc ttgtcgccca cggccaccca cccgggtccg
     5461 gcggcgaccc ggtcgtcgcc gtcctcccag ggctcacccg gctcggccac caggcgcttg
     5521 acgccgatga ggtcgtgctc ccagggcagc gcgatcaccg cgatccggct gcgtcgggga
     5581 cggaggaagc ggccgaggat cgagccggcc cgcacggaga tgacggtgtc gcgagggcgc
     5641 agcgcgggca ccatcgactc gccgcggacg aggtggcgct ccagcgagga ggccgccgcg
     5701 acgccgaccg cgacccccgt ggcggcgccg atcagcgccg ctagggtccg ggcacgagtg
     5761 tccatcctca ccatgatccc caaggagctc ccatgagcat tgtgtcccga ctgctctccg
     5821 tcgccgacct cgacgatgct gccacggcag acgcccactg cgacctgatg tgcggcgtgt
     5881 acaaccccgc gcaggcccgc atcgaggccg agtccgtcct cgagacgatg aagaagtaca
     5941 acgcgtcgga cgacgactac ttcaagaccc gctgcatcat catcaaggag cagcgcgccc
     6001 agctcgccaa ggagcacctg tgggtgctgt ggaccgacta cttcaagccg ccccacgtcg
     6061 agcagttccc ccagctccac gacctgttct ggcgggccac caaggccgcc ggcgacgcca
     6121 agaagagcaa cgacgaggcc gacgcccaga agctgctcga cctcatcgac gagatctccg
     6181 agatcttcgc cgagaccaag aagtaatcag cgcgaggtct cgaagctcga gcgacgctcg
     6241 tggcgctcca gtgcgagcgc cacgagctcg tcgagcaggc ggccgtaggg caggccgctg
     6301 gcctcccaga gcttggggta ctgggagatg ggtgtgaacc cggggatcgt gttgacctcg
     6361 ttcacgaggg gtccacgacc gtcctcctcc cagaagaagt cgacccgcgc catgccctca
     6421 cagcgcaggg cggcgaaggt cgccagcgcg atctcccgca gctcgtcgct catcggcgcc
     6481 gagatcggtg cggggatgag cagctcggcg ccgtcttcgt acttgtcgtc gtagtcgtag
     6541 aagtcggccc ccgggcggac ctcgccgacg accgaggcgc ggggcgtcag gttgccgagc
     6601 acgccgcact ccagctcacg accgacgatg ttctcctcga acaggatcca ctcgtcgaag
     6661 cgcgatgcct cggccacggc ggccgccagg tgttcgacct cgtgcacctt cgagatgccg
     6721 atcgaggagc ccatgttcgc cggcttgacg aacatcggga agccgagccg gtcggcggct
     6781 cgctcgagca gctcgtcgtc gatctcgtgg cgccgggccg acatccacgc gccctggggg
     6841 atgtcgtggg ccgagagcag ctccttggcc gccaccttgt ccatcgacac cgacgagctg
     6901 agcacgccgc tgcccacgta gggcacgtcg gccatctcga gcaggccctg gacggtgccg
     6961 tcctcgccga aggggccgtg cagcaggggc agcaccacca ccgggatgtc tgggccaccg
     7021 ggtgcggccg gctggggctg cagcgcggtg accggttcga cgggggtgcc ggccacgacc
     7081 agctggtcgg gcagcgggcc ttccgtctgc aaccaggagc cgtcgcgggc gatgcccacc
     7141 ggcacgggtt cgtagcggcc ggggtcgagg gcgcgcagca cgtgcgtggc ggtggtgcag
     7201 ctcacctcgt gctcggccga acggcccccg aagagcatga cgagccggat gcggtcgggg
     7261 agggccatgg cgccggagtg tagggagccg gctgcaagaa tcccgggtgt ggagctcagc
     7321 atcgacgagg tcgccgccgc cgtccgcggg agggtggtcg gcccgcccgt caccgtgtcc
     7381 agggtggcca tcgactcgcg cacggtgggt gaggggcagc tgttcgtccc gctggttgcc
     7441 gagcgcgacg ggcacgactt cgtgccgtct gcggtctcgg ccggcgcgtc ggcctacctc
     7501 accgaccgtg gccccctcga cgacggggcc acggccgtcc acgtcgacga cacgatggaa
     7561 gccctgacgc ggctcggcgc cgtggcccgc gaccgactgc ccgacgcggt ggtgggcatc
     7621 accggctcgg tgggcaagac gtcggtgaag gacctgctgg ccggcgtgct cgcgaccacc
     7681 taccgcacga cggccagcga gaagagcttc aacaacgaga tcggcgtccc gctcaccctg
     7741 gtcaacgccc ccgccgacac cgaggtgctc gtgctcgaga tgggggcgag gggcatcggc
     7801 cacatccgcg acctgtgcga ggtggcccgc ccgtcggtcg ccgtcgtgac gatggtggcc
     7861 gaggtccaca cctccgagta cgagaacggc ctcgacgacg tggcccgagc caagggcgag
     7921 ctggtcgagt ccatcccggt ggacgggcac gccgtgctca acggcagcga cgcccgcgtc
     7981 cggcggatgg caggcctcac cgatgcgcgg gtgctcacct acggggtgtc cggcgacgtc
     8041 gtcgcccagc gggtcgacct ggacgaccac ctccgcccac ggttccggct gtcgtcgccg
     8101 tggggcaccg tcgacgtgca gctcgccgtc cacggcgtgc accaggtcgg gaacgccctc
     8161 gccgcggccg cggcagccct ggtgcagggc gtgcccctcg acggggtggc cgaggggctc
     8221 gcatccgccg acctgtcgcc gtggcgcatg gagctgcgcc acaccgacgc cggcgccgtc
     8281 gtggtcaacg acagctacaa ctccaacccc accgcgctcg aggccgccct gcactcgctg
     8341 gccgccatcg acgccgaccg ccacgtcgcc gtcctcgggg tcatggccga gctgggccac
     8401 cacagcgacc agcgccacgc cgagatgggc gagctcgccg aggagctcgg catcgaggtg
     8461 atcaccgtgg actgcccggc ctacggcgtc ggcaccgacg tccccacccc ggaggcggcg
     8521 gtcgacgcgc tcggctcact cggccccgac gacgccgtgc tggtcaaggc gagccgctcg
     8581 gccggcctgg agcgcgtcgc cgccctgctc ctcggctgac caggtgctca gctggcgaag
     8641 acggcgacca gcaccggcgc gatgacgagg gcgggcagga acgagccgac cttgaccttc
     8701 ttgaggtcga gcaggcgcag gccgatgccg aggatcatca cgccgccggt ggcttcgagc
     8761 tcggccacca tccggtcgtc gagcagcgag tcgccgaggg tggccccgag cagggtgatg
     8821 gcgccctgga gcacggcgat gctgagggcg ctgaagccga cgccccaccc gtagacggcg
     8881 gcgaagggca ccgagacgat cccgtccaac gtcgccttca cgatcaggag ctcgctgtcc
     8941 ccgctgatgc cgtcctgcag ggaccccacg atcatcatcg ccccgatgcc gaacgtgagg
     9001 ctggcgatca ggaacccgtc gacgaaggac cgacctgtcg gcacgtgccg gtcgacctcg
     9061 agctcgtcgg ggtcgcgctc gacgtagctg gcgatcacgg gctcggaggc gggaccttcg
     9121 acgaggcgtc ggagccgctc gcccaacccc tcgagccggt cctcgatgcg gagcagctcg
     9181 ccgagcacgc cgccggcgac gatggcgacc acggggaaca cggcgttgtc ggtctcgagg
     9241 aagctggcga tgccgacgcc gagcacgatg agcccgatgc cgtcgagcac cgtggagcgc
     9301 acccgctcgg gcatgcgtcc gtggagcagc acgccgacgc cggtgccggc gagcaccgtg
     9361 accacgttga cgaccgttcc gagaccgcgc accaaccgac cgtagcgacg cgcccgcgcg
     9421 actccagcac tgccagtttc ccgggggtca ggcgatcagg aggctgcagc gagcacagct
     9481 ggcaacccgt tgcccatgag gtcgacgaag ttgcgccggt agaagcgatc gcggtcgtcg
     9541 tcggagacac cggcggcgtc gaggctggtc tcgaagcgct cgaccgggcg ccggccgccc
     9601 tcgacgtgcg ggaagtcggt gctgaacagc acggtctcgg ggccggcctg ctcgatgatc
     9661 caccccacgt cctcggtggg gtacggcgtg aaccgcatct ggcgcgacac gtactccgac
     9721 ggacgcatcg acagctgctg gaggcgctcc tcgtggcggt ggaaggcgtc gaaggccgac
     9781 tccatctggc gcatccagcc cggcacccag atcgcgccct gctcgatgac gccggcgcgc
     9841 aggtccggga atcgctcgaa cacgccgtcg aagagcatcg tggccagcgt ctgggccggc
     9901 ggccccggga tgcccatgta gtcgacgcag cggaagttct cgtcgccccc gtggaagtcg
     9961 ggcgggatgg gcagcccgtt ctggaagtac atcggatcga tgagctcgcc ggtgccgccc
    10021 acgtggaaca cgatggggat gccggcctcc tgggcctggg cccacaccgg atcgagcgag
    10081 cggtggctgg gggagtgggt ggccgggcag cccgacggga tcagcagggc cgctgcgccg
    10141 aggtggaggg cctcgccggc catggccgcg gcccggtcga agtcggcaag cggcacgtac
    10201 atggtcgaga gcaagcgctc gtcgaccgag cagaactcgg ccatgccgcg gttgtgggcc
    10261 cgggcggtgc cgtaggccat ctcgaggtcc ccgctgtgct cccaagcgaa cagccgcgag
    10321 ttgtggaagg tgttgaacac gagctggctc tgcacgccga tcaggtccag cgctcgaacc
    10381 cggtcctccg ggatgaacga gccggtggca gcgaagttct tgcgcagcat gatctcgtgc
    10441 gcctcctcgg cccggtactc ctccgaggcg tgcttggcgg cgaggcggtc gaaggcggcg
    10501 gtgaggtcgg cgagctgctc ctcgacgtca ccggtctggc gcagctcgtt gccgctcgtc
    10561 agcgggagcg gctcgatgcg gtcgcggatg gccgggtcgg cgtggtcgcg caaccaggtc
    10621 ggcgtctcca tgatgtgggc gtccgcgtcg tggacgaccc gatcggcgat gtacggcatg
    10681 cgatcccccc ggatgcgagc tcaggcctgc ttcggtcgca ggtccgccca ccatgccagg
    10741 aacagggcga cgaacgcacc agggaacacg acggtggtga ccgaggtggc gtcgatgatc
    10801 gggccggcca ccaggttgcc gaggggcacg gtgccgccga aggccatcat ccacagcgcc
    10861 atgacccggc cccgctcgtg gccggcgagg tgcatctgca gcaccgtgga cagggcggtg
    10921 acggtggcga agtagaagaa cccgaccacg atgatgacga ggtaggcggg caccggcgtg
    10981 cgcagccagg cgaacacgcc gagggcgatc ccgaaggcga tcatgcccca gcgggtgacg
    11041 tcctccttgg gcacctcgga gaacgtcgtg ccgaccgaga gggcgccgag catggcgccg
    11101 aggccgaagc aggcgtacag ccagccgtag gccgacgacg tggcggcgat gcccaggttc
    11161 cgctcggcga gggcgggcat ctggccgacg aagggcaggc acaggaacga gaacacgatc
    11221 atggtgacca gcgaccggcc gatggggaag cggttgcggg cgatgcggaa cccgagtgcg
    11281 agccgctcga tgcgggactc accggctccg gccgtctcca cgtcggggag tcggatcgag
    11341 gccagggcgc cgatgacgaa caggtaggtg gcggcgttga ggctgaacac gacctcggcg
    11401 ccgtagcggg cgaagagcag gccgccgatg gcagggccga tcacgcggga cccgttcatc
    11461 tgcgtcgagt tgagcgagat ggcgccggcc aggttgccgg gcccgacgag gtcgggcagg
    11521 acggcggagt aggtgggtcc gaagaccgac tggccgagtc cgatgaggaa cacgacgccg
    11581 acgatcgccc accggttcgg gtcctctccg gcgacgacga cggccaggac cacggcgagc
    11641 gccgcctgcg tcgccgagac gatcatcagc aggtggcgac ggtcgaagga gtcggccagc
    11701 atcccgccga tgatcgagaa gaacagcatg ggaccgagct gggcgaacag cacgatgccg
    11761 acgaacgagg acgagccgcc gctgatgtcg taggcgagcg cgccgagcac gacgttctgc
    11821 atccacgtgc cgatgttgga gaagaacgcg ccggtgtaga cccgccggaa cgtctgctgg
    11881 cgcagggccg ccatcgactg gccgccccac tgtggaccct cctcgtcacc ctcctcgccg
    11941 tcctcctcgg tggcgtcggc aggagcggtc accgggacag tctgcccgcg accctcggga
    12001 actgaccaac gggtcaagtc cagctaccgg aacacgtgtt cgagcgccgg gtgtaccctc
    12061 ggaccgtgag ctcggccggg atcctgcacg ccgacctcga ctgcttctac gccgcggtcg
    12121 agcagcgcga cgacccccgc ctgcggggcc ggccggtcat cgtgggcggt ggcgtggtcc
    12181 tcaccgccag ctacgaggcc cgggtccacg gggtccgcac cgccatgccc gtgccgcagg
    12241 cccggtcgct gtgcccgtcc gcgatcgtgg tgccctcccg gatgtcggcc tacaccgagg
    12301 cgagtcgcgc cgtgttcgcg atcttccggg agaccacccc gctggtggag ggcctctcgg
    12361 tggacgaggc cttcctcgac gtgaacgggc tgcgccgcct cgtcggcgag cccatcgaga
    12421 tcgcccgtga gcttcgccgg cgggtgcgcg atgaggtcgg gctccccatc agcgtcggcg
    12481 tcgcccgcac caagttcctg gccaaggtgg ccagcgccgt gtccaagccc gacgggctcc
    12541 tcgtggtcga gcccgacggc gagctcgact tcctgcatcc cctcgacgtg cgccggctgt
    12601 ggggcgtcgg tccggtgacc gccgggaagc tggacgagcg aggcctgcgc accgtcggcg
    12661 acgtcgccgg cgtcccggag tcggccctgg tcgccatgct cggccctgcc gccggacgtc
    12721 acctgcacgc cctggcccgc aacgtcgacc cgcgctcggt cacgccgggg acgcgtcgca
    12781 gctcgatcgg cgcccagcag gccctcagtc gcgcaggcgg ccgacgtccg tggtctcgcg
    12841 ccgagctcga cgccgccctg ctcgccctgg tcgagcgggt caccgagcgc ctccgcggtg
    12901 gccggcgggt cggccggacc gtgacgctgc gcctgcgcca tgccgacctc agccgggaga
    12961 cccgctcccg caccctgccc gtcgccaccg accgcaccga cctgatcctc ggcgcggccc
    13021 gcgacctgct cgatgcccgc tggtcgcacc tgcgcgaggc cgggtgctcc ctcatcggcg
    13081 tcaccgtctc cgagctggcc ccggccgacg ccgtgcagct gagcctcgac ctcgacggcc
    13141 acgaccgccg caccctcgac cacaccctcg acgccgtccg cgaccgcttc ggcggcagcg
    13201 ccgtcacccg cgcatcgctc ctcggcgagg gccgcggcgc ccgggtcccg atcctcccgg
    13261 accccgtccc cgacccctga ccgcgagcgt caagatggtg ggcatggctt ccgacgctgc
    13321 ccgcatcctc gtcctgcccg ccggcggccg cccgtggctg gccgatgctg tccgtgccgg
    13381 gggcggtgag ccggtcaccg acgggcctgc cggcgccgtg gtctggaccg atcc
//
LOCUS       DATISU010000401         3051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_825651, whole genome shotgun sequence.
ACCESSION   DATISU010000401 DATISU010000000
VERSION     DATISU010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825651"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1548
                     /locus_tag="VLR27_15975"
     CDS             142..1548
                     /locus_tag="VLR27_15975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HSP05010.1"
                     /translation="MERLSALDAEFLLVEDGIAHMHIAGISVFEGPRPELSELTAMVA
                     AKLHRIPRYRQRVRTVPLELGRPVWVDDPHFDLGYHIRHTALPAPGDDAALRRLMSRL
                     MGQELDRARPLWEVWFVEGLEDGRWALISKVHHCMVDGVSGMDLLGILLDVEPDAPLP
                     EAPEWSPEPEPAGIAKVLDAWSGALADGLGLAARAPSALRDPKRAVSSLLDTGRGLLE
                     LTRHLGLTTPTTLEGSIGPHRSWAHAVVPLDDVKALRQELDTTINDVVLALLAGAFRD
                     LLLHRGEDPDDCVLRTLVPVSVRTEDARGVPDNRVSAILFELPVHLADPVARVQAVKE
                     QMAERKASHMSEAGEVVVRLGDLAPPMLVGSVMRTATRLLARMPQRSVNTVTTNVPGP
                     QLTLYCLGRPMLVHLPYVPLSHGVRIGVAILSYDGNLAFGVTGDRDTAADVDVLAGAV
                     PKGYEELKTRLSGNVPVS"
     gene            1564..2346
                     /locus_tag="VLR27_15980"
     CDS             1564..2346
                     /locus_tag="VLR27_15980"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HSP05011.1"
                     /translation="MDNDPRRFGRITRGERAVAGVAALVLLVLVVVEPDIVGAPFENA
                     RTVAFTVGGTVVAAVLLALMLRFDVAPVVRIIVLVVPFVAVSWWLLAPFFVDDVVDEE
                     FATSIAAELEGGDGPGTDSTGTTSSTAPAAPDGPTPMQETATEPSGPVLLAAGSFVGL
                     AGHSGTGDAGIFENPDGSLALRFEAFDIENGPDLEVYLVPGADQTSLAAGSVHLGALK
                     GNVGDQTYELPPGTELPPGSYTALVWCEAFAVEFVGATLTIG"
     gene            complement(2384..>3051)
                     /locus_tag="VLR27_15985"
     CDS             complement(2384..>3051)
                     /locus_tag="VLR27_15985"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HSP05012.1"
                     /translation="PDELGSALVFLTGPPEEFVPVHLQGQTLAAVALLWAGDVPDGED
                     AIADLRALGPEVDLVGPMPYSEFQCMIDDPPGLGNYWSADYHDSFPDEALDTFVKYGF
                     ERPSPVSQQILVPWGGAIARVADDATPMTHRDVRWISHPFAMWDASEDATANIEWAQS
                     FRRDIAAHTNGGVYLNFIGHEGEERIKAAFGEDKYRRLQQVKADWDPDNVFQGNQNIR
                     PAG"
BASE COUNT          405 a         1081 c         1086 g          479 t
ORIGIN      
        1 gtcgagcgtc ttcgggaggt cgggatcgaa ggtcatgtcc ctccgacgtt gccaccgccg
       61 ccagatcatc gccacgaggt ctgcgccgga aagggtccgc agtccctgtc gccgcccggg
      121 agcccttcgt acggtcggac gatggagaga ctgagcgcgc tcgatgccga gttcctcctc
      181 gtcgaggacg gcatcgccca catgcacatc gccgggatct cggtgttcga gggtccccgg
      241 ccggagctgt ccgagctgac cgcgatggtg gcggccaagc tccaccggat cccccgctac
      301 cggcagcggg tgcggaccgt gcccctggag ctcgggcgac cggtgtgggt cgacgacccg
      361 cacttcgacc tcggctacca catccggcac accgccctgc ccgcccccgg tgacgacgcc
      421 gcgctgcgac ggctgatgtc ccggctcatg ggccaggagc tcgaccgggc gaggccgctg
      481 tgggaggtgt ggttcgtcga gggcctcgag gacggtcggt gggccctcat ctccaaggtg
      541 caccactgca tggtcgacgg cgtctccggc atggacctgc tcggcatcct cctcgacgtc
      601 gagccggacg cgccgctgcc tgaagcgccg gagtggtcac ccgagcctga gcccgccggg
      661 atcgccaagg tgctggacgc gtggtcaggt gccctggccg acggcctcgg gctggccgcc
      721 cgggcaccga gcgcgctgcg ggatccgaag cgagccgtgt cctcgctcct cgacaccggt
      781 cgcggcctgc tcgagctcac caggcacctg ggtctgacca cgcccaccac gctggagggc
      841 tcgatcgggc cgcaccggag ctgggcccac gccgtggtcc cgctcgatga cgtgaaggcg
      901 ctccgccagg agctggacac cacgatcaac gacgtcgtcc tcgccctgct ggccggcgcc
      961 ttccgcgacc tgctgctgca ccggggcgag gaccccgacg actgcgtgct gcggaccctc
     1021 gtccccgtgt ccgtgcgcac cgaggacgcc cgtggcgtgc ccgacaaccg ggtgtcggcg
     1081 atcctgttcg agctgcccgt gcacctggcc gaccccgtgg cgcgggtcca ggcggtgaag
     1141 gagcagatgg ccgagcggaa ggcgtcgcac atgagcgagg ccggcgaggt ggtcgtgcgc
     1201 ctcggtgacc tggcaccacc gatgctggtg ggctccgtga tgcggacggc gaccaggctg
     1261 ctcgcccgca tgccccagcg gtcggtgaac accgtgacga cgaacgtgcc cggcccgcag
     1321 ctcacgctgt actgcctggg ccgaccgatg ctggtgcacc tgccgtacgt gccgctgtcg
     1381 cacggcgtcc ggatcggcgt ggcgatcctg tcctacgacg ggaacctcgc gttcggcgtc
     1441 accggcgacc gcgacaccgc agccgacgtc gacgtgctcg ccggcgccgt gccgaaaggt
     1501 tacgaggagt tgaagacccg gctcagcgga aatgtgccgg tgtcgtgaca cgtttccgtc
     1561 cccatggaca acgacccgcg tcggttcgga cggatcacga ggggcgagcg ggccgtcgcc
     1621 ggggtcgccg ccctggtgct gctggtgctg gtcgtggtgg agcccgacat cgtcggcgcc
     1681 ccgttcgaga acgccaggac cgtcgcgttc accgtcggtg ggacggtggt cgctgcggtc
     1741 ctgctcgccc tcatgctccg gttcgacgtg gcgccagtgg tgcgcatcat cgtcctggtc
     1801 gtccccttcg tcgccgtgag ctggtggctg ctggccccgt tcttcgtcga cgacgtggtg
     1861 gacgaggagt tcgcgacctc gatcgccgcc gagctcgagg gcggtgacgg accggggacc
     1921 gacagcaccg ggacgacgtc gtccactgcg ccggccgccc ccgatggtcc aaccccgatg
     1981 caggagaccg caacggagcc gtccggtccc gtcctgctcg ccgccgggtc cttcgtcggg
     2041 ctcgccgggc actccggcac cggcgacgcc ggcatcttcg agaaccccga cggctccctc
     2101 gccctgcggt tcgaggcctt cgacatcgag aacggcccgg acctcgaggt gtacctcgtg
     2161 ccgggcgccg accagacgtc cctcgccgcc ggctctgtcc acctcggtgc gctgaagggc
     2221 aacgtgggcg accagaccta cgagctccct cccggcaccg agctgcctcc cgggtcctac
     2281 accgcgctcg tctggtgcga ggcgttcgcc gtcgagttcg tcggcgccac cctcaccatc
     2341 gggtgacgcc gccggggcag ccgctggcca gcgcagcgcg tgctcacccg gcgggccgga
     2401 tgttctggtt gccctggaag acgttgtccg gatcccagtc ggccttgacc tgctgcaggc
     2461 gccggtactt gtcctcgccg aaggccgcct tgatgcgctc ctcgccctcg tggccgatga
     2521 agttgaggta gacgccgccg ttggtgtgcg cggcgatgtc tcggcggaag ctctgggccc
     2581 actcgatgtt ggcggttgcg tcctcggagg cgtcccacat ggcgaagggg tggctgatcc
     2641 agcggacatc gcgatgggtc atcggcgtgg cgtcgtcggc gacacgggcg atggctccgc
     2701 cccaggggac gaggatctgc tggctgacgg gtgatggccg ctcgaagccg tacttgacga
     2761 aggtgtccag cgcctcgtcc gggaacgagt cgtggtagtc ggcgctccaa tagttcccga
     2821 ggccgggcgg gtcgtcgatc atgcactgga actcgctgta gggcatgggg ccgacgaggt
     2881 cgacctcggg cccgagcgcg cggaggtcgg cgatggcgtc ctcaccgtct ggaacgtcac
     2941 ccgcccacag gagcgcgacc gcagcgaggg tctgcccctg caggtggacc gggacgaact
     3001 cctccggtgg cccggtcagg aacacgagcg ccgagccgag ttcgtccggt g
//
LOCUS       DATISU010000402         4784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_779190, whole genome shotgun sequence.
ACCESSION   DATISU010000402 DATISU010000000
VERSION     DATISU010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779190"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VLR27_15990"
     CDS             complement(<1..203)
                     /locus_tag="VLR27_15990"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP05013.1"
                     /translation="MGAISTTDAPGPVAPVAPATEERIRTGALACIARTGITRTTVDD
                     VVREAGVSRATLYRAFPGGKDVLV"
     gene            complement(265..855)
                     /locus_tag="VLR27_15995"
     CDS             complement(265..855)
                     /locus_tag="VLR27_15995"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP05014.1"
                     /translation="MTSRAALAGRVLDATFAEVAEHGLAALTVEAVAVRAGSSRATLY
                     RHFPGGRDELVARTIEREVERFFAAVVADVPPAAAGVVDHVAGIVVSARRLLGEHDVL
                     QRLLVDEADAIVPSLATVQPLVATGLARHLTAALGEAVARGEVRSDVDVAMAGDHAAR
                     LVLRYVGSPGRWDLVDPGEARRLVQDRLLAGVVRDG"
     gene            complement(852..1604)
                     /locus_tag="VLR27_16000"
     CDS             complement(852..1604)
                     /locus_tag="VLR27_16000"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HSP05015.1"
                     /translation="MYRFALRPLWILSHLFALACVVAFVGLGLWQLDRHDQRAERNAT
                     IEARADLPADPIADALRDAGDDDEALRFRTVAASGTYGEGDLLVDNRSKDGLPGAWVL
                     TPLVLDDGTTLVVNRGFQFNDGGRVDPEPAPDGPVSIEGTATTWEDDGCGVRRDDAGE
                     PVGMACLRRSAAEEAFGGDVLPVVVQRQTSTPPAADALAPVPLPELDAGPHRSYAVQW
                     FIFAALTAIVYVLILRRVAHRVGERADDADGA"
     gene            complement(1650..2624)
                     /locus_tag="VLR27_16005"
     CDS             complement(1650..2624)
                     /locus_tag="VLR27_16005"
                     /inference="COORDINATES: protein motif:HMM:NF039623.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HSP05016.1"
                     /translation="MRTTKAIVAALVAALLFGTAAPAGAGLLSGSDSDTSTTSSYDDP
                     FFLNWPTYLRSTSPDTYVASTSNECKNGQVQCVDKVIREMTSRYNALGCDHDSTFAFT
                     YLLTTEEYRRAVEDPQFFTDNAFINHQDAVFADYYFEQFDDWHRGAVEQVSPAWRIAF
                     DSAERQLVSGMGNVFLGMNAHVNRDLPFVLADIGLTKPDGTSRKADHDRVNDFLAATN
                     QYLLSEAAKVLDPTLDDGDIPGTSVDNSVMVQALVVWREQAWRNAERLVAAPTPEARA
                     RVAEEIEQMAAAEALLIRTSFAYTPLHRLLGMGPTQRNAFCEANFRTR"
     gene            2783..3517
                     /locus_tag="VLR27_16010"
     CDS             2783..3517
                     /locus_tag="VLR27_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05017.1"
                     /translation="MTDQPALARHPPSPHTLKLLVAPVIFLVICSNVANVMWPTLQED
                     HPLLLIALSSINRYLILVSDQLDPISYYGVGTLRLLVSDPLFFLLGYWYGDRALAWMD
                     QKSPGYGPMLRKAQQWFGKAAWPLVLIAPNNFICLFAGAAGMRVGWFFALNITGTVGR
                     LYLIRVLGEAFSSPIDSFQGVVAEYRWWFIAGSALFVLISLRGEVKGGGEIDQLVHID
                     EELAEPAEAAGETGDEAPEPRVEEER"
     gene            3514..4251
                     /locus_tag="VLR27_16015"
     CDS             3514..4251
                     /locus_tag="VLR27_16015"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSP05018.1"
                     /translation="MSERRVAVVTGASAGIGAATARLLAADGWHVVLGARRVDRIEAL
                     AAELDGTAIALDVTDRASVDAFVDAVERCHLLVNNAGGALGVEPLAEAVDEKWISMFE
                     SNVLGLMRMTRGLLPRLVASGDGHVVNVTSIAGLQGYPGGSGYNAAKFGAHGVTESLR
                     HELLGQPVRVTEILPGLVETEFSVVRFDGDADRAAQVYAGLQPLTADDVAECIRWAVA
                     RPSHVNIDSIVVKPRAQADAFRTHRES"
     gene            4251..>4784
                     /locus_tag="VLR27_16020"
     CDS             4251..>4784
                     /locus_tag="VLR27_16020"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05019.1"
                     /translation="MTATIRRQSAHPPLGARTPARNEDFFDEDQLAELRAYSRPRKKV
                     DLAAKIVVGAIDLVLIFVLDLGPEVAGWVDGAPWPLQLVVVAGLFALISQVLMLPADW
                     WATMVHDKRHGLSNQSAGLWAGDQVKGLVLNVVLSALLLVPIFWAIRTFDTWWLVGGL
                     LFLGIALFLNFVYPVLIM"
BASE COUNT          665 a         1721 c         1697 g          701 t
ORIGIN      
        1 accagcacgt ccttgccgcc ggggaaggcg cggtacagcg tggcccggga gaccccggcc
       61 tcacggacga cgtcgtcgac cgtggtgcgg gtgatgccgg tgcgggcgat gcaggcgagg
      121 gccccggtgc ggatgcgctc ctcggtggcc ggcgccaccg gagccacggg ccccggtgca
      181 tcggtcgtcg agatcgcccc catcaccatg agacactacg ggctgatctg tctcactgtc
      241 tacgcaaatc ggacgtgacg tccgtcaccc gtctcgcacc acgccggcca ggagccggtc
      301 ctgcaccagc cgccgggcct caccggggtc gaccaggtcc cagcggcccg ggctgccgac
      361 gtagcggagc acgaggcggg cggcgtggtc gccggccatg gccacgtcga cgtcggaccg
      421 gacctcgccc cgggccaccg cctccccgag cgccgcggtc aggtggcggg cgaggccggt
      481 cgccaccagc ggctggacgg tggccagcga cggcacgatg gcgtcggcct catcgaccag
      541 caaccgctgc agcacgtcgt gctcgccgag cagccgccgg gccgacacga cgatgccggc
      601 gacgtggtcg accacgccgg ctgccgccgg cggcacatcg gccaccaccg cggcgaagaa
      661 ccgctcgacc tcgcgctcga tcgtgcgggc gacgagctcg tcccgtccac cggggaagtg
      721 ccggtagagg gtggctcggg acgagccggc ccggacggcg accgcttcaa cggtcagcgc
      781 ggccaggccg tgctcggcga cctcggcgaa ggtggcgtcg aggacccggc cggcgagggc
      841 cgcgcggctc gtcacgcgcc gtccgcgtcg tccgctcgct cgcccacgcg gtgggcgacc
      901 cgacgcagga tgaggacgta cacgatggcg gtgagggcgg cgaagatgaa ccactgcacg
      961 gcgtagctgc ggtgcggccc ggcatcgagc tcgggcagcg gcaccggcgc gagggcatcg
     1021 gccgcgggcg gcgtcgacgt ctggcgctgc accacgacgg gcaggacgtc gccgccgaac
     1081 gcctcctcgg cagccgaccg ccgcaggcac gccatgccga ccggctcgcc ggcgtcgtct
     1141 cggcgcacgc cgcagccgtc gtcctcccag gtcgtggcgg tcccctcgat cgacaccgga
     1201 ccgtccggcg ccggctcggg gtcgacccga ccgccgtcgt tgaactggaa gccccggttg
     1261 accaccaggg tcgtgccgtc gtcgaggacc agcggggtca ggacccaggc accgggcagg
     1321 ccgtccttcg agcggttgtc gacgagcagg tcaccctcgc cgtacgtgcc ggacgccgcg
     1381 acggtgcgga agcggagcgc ctcgtcgtcg tcaccggcgt cccggagcgc gtcggcgatc
     1441 gggtcggcgg gcaggtcggc tcgggcctcg atggtggcgt tgcgctcggc ccgctggtcg
     1501 tgacggtcga gctgccagag ccccaggccg acgaaggcga cgacgcaggc cagcgcgaac
     1561 aggtgcgaca ggatccacag ggggcggagg gcgaaccggt acaccgcggc cgagactacg
     1621 gaccgaggtc atcgcggcgg gattcgccgt cagcgggtgc ggaagttggc ctcgcagaag
     1681 gcgttgcgct gggtcggccc catgccgagc aggcggtgca gcggcgtgta ggcgaacgag
     1741 gtgcggatca gcagcgcctc ggcggccgcc atctgctcga tctcctcggc gacccgggcc
     1801 cgggcctcgg gggtcggggc cgccacgagc cgctcggcgt tgcgccacgc ctgctcccgc
     1861 cacacgacga gcgcctgcac catgaccgag ttgtcgaccg aggtgccggg gatgtcgccg
     1921 tcgtccagcg tcgggtcgag gaccttggcg gcctcggaca gcaggtactg gttggtggcg
     1981 gccaggaagt cgttgacccg gtcgtggtcc gccttgcggc tggtgccgtc gggcttggtc
     2041 agaccgatgt cggcgagcac gaagggcagg tcgcggttca cgtgcgcgtt catgccgagg
     2101 aagacgttgc ccatgccgct caccagctgg cgctcggcgc tgtcgaacgc gatgcgccac
     2161 gccggcgaca cctgctcgac cgcaccccgg tgccagtcgt cgaactgctc gaagtagtag
     2221 tcggcgaaca ccgcgtcctg gtggttgatg aacgcgttgt cggtgaagaa ctgcgggtcc
     2281 tcgacggccc ggcggtactc ctcggtggtg agcaggtacg tgaaggcgaa cgtcgagtcg
     2341 tggtcgcagc ccagcgcgtt gtaccgggag gtcatctcgc ggatgacctt gtcgacgcac
     2401 tggacctgac cgttcttgca ctcgttgctc gtcgacgcca cgtaggtgtc ggggctcgtc
     2461 gagcgcaggt aggtcggcca gttcaagaag aacgggtcgt cgtagctcga cgtcgtgctc
     2521 gtgtcgctgt cggagcccga cagcagtccg gcaccagccg gcgccgccgt cccgaacagc
     2581 aacgccgcca ccagggcggc cacgatggcc ttggtcgtac gcacccgcga ttccccctcc
     2641 gcgtcctctc tccgccacac accgtcacat gccgtgacag ttgacgacca tggcccgttc
     2701 gggtggtttc cgatccaccc gaacgggtcg gtcgatcacc ccggcggtcc cgtgcgcggg
     2761 ccgcctgcaa gactcgtcgc ccgtgaccga ccagccagcc ctggcgaggc acccaccgag
     2821 cccgcacacg ttgaagctcc tggtcgcgcc cgtgatcttc ctggtgatct gctccaacgt
     2881 cgccaacgtc atgtggccga cgctgcagga ggaccacccc ctcctgctca tcgccctgag
     2941 cagcatcaac cgctacctca tcctggtgtc ggaccagctc gacccgatca gctactacgg
     3001 cgtcggcacc ctccggctgc tggtctcgga ccccctcttc ttcctgctcg gctactggta
     3061 cggcgaccga gcgctggcgt ggatggacca gaagtcgccc ggctacgggc cgatgctgcg
     3121 caaggcccag cagtggttcg gcaaggcggc ctggccgttg gtgctgatcg caccgaacaa
     3181 cttcatctgc ctgttcgccg gggcggccgg gatgcgcgtc gggtggttct tcgccctgaa
     3241 catcaccggc accgtcgggc gcctctacct gatccgggtg ctcggcgagg cgttctcctc
     3301 ccccatcgac agcttccagg gcgtggtcgc cgagtaccgc tggtggttca tcgccgggtc
     3361 ggctctgttc gtgctgatca gcctgcgggg cgaggtcaag ggcggcgggg agatcgacca
     3421 gctcgtgcac atcgacgagg agctggccga gccggccgag gccgccggcg agaccggcga
     3481 cgaggccccc gaaccgcggg tcgaggagga gcgatgagcg agcgacgggt cgccgtcgtc
     3541 accggggcga gcgccggcat cggtgcggcc accgcccgcc tgctggcggc ggacggctgg
     3601 cacgtcgtgc tcggcgcccg gcgggtcgac cggatcgaag cgctcgcggc cgagctggac
     3661 ggcaccgcca tcgccctcga cgtcaccgac cgcgcgtcgg tcgacgcctt cgtggacgcg
     3721 gtggagcgct gccacctgct ggtgaacaac gccggcggcg ccctcggcgt cgagccgctc
     3781 gcggaggccg tcgacgagaa gtggatctcg atgttcgagt ccaacgtgct cgggctcatg
     3841 cgcatgaccc gggggctgct cccccgcctc gtcgcgtcgg gcgacggcca cgtcgtcaac
     3901 gtcacctcca tcgccgggct ccagggctac cccggcgggt cgggctacaa cgcagccaag
     3961 ttcggtgcgc acggtgtcac cgagtcgctc cgacacgagc tgctcggcca gcccgtgcgg
     4021 gtcaccgaga tcctccccgg cctggtggag accgagttca gcgtggtgcg cttcgacggc
     4081 gacgccgatc gcgccgccca ggtctacgcc ggtctgcagc ccctgaccgc cgacgacgtc
     4141 gccgagtgca tccgatgggc ggtggcgcga ccgagccacg tcaacatcga ctccatcgtg
     4201 gtcaagcccc gcgcccaggc cgacgccttc cgcacccacc gagagagctg atgaccgcca
     4261 ccatccgacg ccagtcggcg caccccccgc tgggtgcccg cacccccgct cgcaacgagg
     4321 acttcttcga cgaggaccag ctcgctgagc tgcgcgccta cagccggccc cgcaagaagg
     4381 tcgacctcgc cgccaagatc gtcgtcggcg ccatcgacct cgtgctgatc ttcgtgctcg
     4441 acctcggtcc cgaggtcgcc ggctgggtcg acggggcgcc gtggccgctg cagctggtcg
     4501 tggtcgccgg actcttcgcc ctgatctctc aggtactgat gctgcccgcg gactggtggg
     4561 cgacgatggt ccacgacaag cgccacggcc tgtcgaacca gagtgcgggg ctgtgggccg
     4621 gcgaccaggt caagggcctg gtcctcaacg tcgtgctctc cgccctgctg ctggtcccga
     4681 tcttctgggc catccgcacg ttcgacacgt ggtggctggt gggcggcctg ctgttcctcg
     4741 gcatcgccct gttcctgaac ttcgtctacc cggtgctgat catg
//
LOCUS       DATISU010000403         1616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_221510, whole genome shotgun sequence.
ACCESSION   DATISU010000403 DATISU010000000
VERSION     DATISU010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221510"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..484
                     /locus_tag="VLR27_16025"
     CDS             17..484
                     /locus_tag="VLR27_16025"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HSP05020.1"
                     /translation="MTAPTRADEAQTERLARLLDSQFRVPGTSLRFGVEPLLGLVPGV
                     GDAAGLALSSAVIVQAVRLGARGATVARMVLNVAIDAVFGSIPLLGSVFDFAFKANNR
                     NVALLQRHELDPAGTSVESRRAVRRTLIGVAIGLVVTLILVIALVTWLLTLIF"
     gene            537..683
                     /locus_tag="VLR27_16030"
     CDS             537..683
                     /locus_tag="VLR27_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05021.1"
                     /translation="MSRYTPPSDHDDDITPYRRRSLGMRIGVWVLLIGVLALVFVDVL
                     AALI"
     gene            693..1304
                     /locus_tag="VLR27_16035"
     CDS             693..1304
                     /locus_tag="VLR27_16035"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HSP05022.1"
                     /translation="MQVEVFADVWCPFTHVGLRRFVELRTHMDVPPALVVRSWPLELV
                     NGAPMDPAFIAEEVEDIRGSVAPDLFTGFDPARFPTTTLSALALTGRAYERSPATGEA
                     VALELRDRLFERGEDIGDPDLVAAVATTHGLPADAGADHDLPIRDWEEGKSRGVIGSP
                     HFFTPGGDWFCPSLDVSRADDGHLRITDDPEGFDAFVRSCQEG"
     gene            complement(1311..>1616)
                     /locus_tag="VLR27_16040"
     CDS             complement(1311..>1616)
                     /locus_tag="VLR27_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05023.1"
                     /translation="NDPDVLHLAGFLLLFVAVLQPVNGAVFVLDGLLIGAGDMSFLAK
                     AMVGAALLTAPVAIAVAVLGLGIGWVWGAITVLMLARLGVLALRWRSGAWAVVGAER"
BASE COUNT          220 a          617 c          556 g          223 t
ORIGIN      
        1 gggtagggag cggcccatga ccgcaccgac ccgggccgac gaggcccaga ccgagcgcct
       61 cgcccgcctg ctcgacagcc agttccgcgt gccgggcacg agcctgcgct tcggcgtgga
      121 gccgctgctc ggactggtgc ccggggtggg cgacgccgcc gggctggccc tgtcgtcggc
      181 ggtcatcgtc caggccgtgc ggctcggggc ccgcggcgcc acggtggccc gcatggtgct
      241 gaacgtggcc atcgacgccg tgttcggctc gatcccgctg ctcggctcgg tcttcgactt
      301 cgcgttcaag gccaacaacc gcaacgtcgc cctcctccag cgccacgagc tggacccggc
      361 cggcacgtcg gtcgagagcc gacgagccgt gcgacggacg ctgatcggtg tggcgatcgg
      421 actcgtcgtg acgctgatcc tggtgatcgc gctggtgacc tggttgctca ccctgatctt
      481 ctgacgcccc ggtccgacgc gaggtcggga cgcagcccca ccggtacggt ggccacgtgt
      541 ctcgctacac gcccccgtcc gaccacgacg acgacatcac cccgtaccgg cgccggtcgc
      601 tcggcatgcg catcggcgtc tgggtcctgc tgatcggcgt gctggcgctg gtcttcgtcg
      661 acgtcctggc cgccctgatc taggagcaac ccatgcaggt cgaggtcttc gccgacgtct
      721 ggtgcccgtt cacccacgtc ggtctccgcc gcttcgtcga gctccgcacc cacatggatg
      781 tgccaccggc gctcgtcgtg cggtcctggc cgctcgagct ggtgaacggc gccccgatgg
      841 acccggcgtt catcgcagag gaggtcgagg acatccgcgg ctcggtcgcg cccgacctgt
      901 tcaccggctt cgacccggcc cggttcccga ccaccacgct gtcggccctc gccctgaccg
      961 gccgggccta cgagcggtcg ccggcgaccg gcgaggccgt ggccctcgag ctccgcgacc
     1021 gcctgttcga gcggggcgag gacatcggcg accccgacct ggtggccgcc gtggcgacga
     1081 cgcacggcct gcctgccgac gccggcgccg accacgacct cccgatccgc gactgggagg
     1141 agggcaagtc ccgcggcgtg atcggctcac cgcacttctt cacgccaggc ggcgactggt
     1201 tctgcccgtc gctcgacgtg tcccgggccg acgacggcca cctgcgcatc accgacgacc
     1261 ccgagggatt cgacgccttc gtgcggagct gccaggaggg ctgagcgggg tcagcgctcg
     1321 gcgcccacca ccgcccacgc ccccgaccgc caccgcagcg ccaggacgcc gagccgggcc
     1381 agcatgagca cggtgatggc gccccacacc cagccgatgc cgaggccgag cacggcgacc
     1441 gcgatggcga ccggggcggt cagcagagcg gcaccgacca tggccttggc caggaacgac
     1501 atgtcgccgg cgccgatcag caacccgtcg agcacgaaca ccgcgccgtt caccggctgc
     1561 agcacggcca cgaacaggag caggaagccg gccaggtgga gcacgtcggg gtcgtt
//
LOCUS       DATISU010000404         1653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_221544, whole genome shotgun sequence.
ACCESSION   DATISU010000404 DATISU010000000
VERSION     DATISU010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221544"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VLR27_16045"
     CDS             complement(<1..762)
                     /locus_tag="VLR27_16045"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HSP05024.1"
                     /translation="MSGPDDTTETTVDIKARSRDVTDGMERAPARAMLRAIGMQDEDF
                     AKAQIGVASSWNEVTPCNMPLDRLAKRSKVGVRDAGGYPIEFVTIAVSDGISMGHEGM
                     RASLVSREVIADSVETVMHAERMDGLLTFAGCDKSLPGMLMAAARLNVPSVFLYAGSI
                     LPGQYKGEALDIVSVFEAVGARAAGTIDQETLDAIERNACPTEGACAGMFTANTMASI
                     SEALGMSLPGSASAPAVDGRRDDLAYESGKAVMELI"
     gene            827..>1653
                     /locus_tag="VLR27_16050"
     CDS             827..>1653
                     /locus_tag="VLR27_16050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HSP05025.1"
                     /translation="MPAFGALVAEPLYVLADTAVVGRIGTDELAGLAVASSALLTGYA
                     IFIFLAYGTTAAVSRLLGAGREGSAAHQAVQGLWLAGVIGVVMTALVLGLADPIVELL
                     GGAGAVKTNALVYLRISAIGITFQLLTFAGTGYLRGLQDTRTPLLVAIGAAVANLVIE
                     LVLINGLGFGIGASALSTVIAQAGGALVYLRLITTATSRLGAALRPDWSAIRRLGRAG
                     WDLLVRTLALRMSLTLFTAIAARIGAEEVGAHEIAFAIWSLLALALDAIAIAGQAMVG
                     "
BASE COUNT          200 a          599 c          591 g          263 t
ORIGIN      
        1 gatgagctcc atgaccgcct tgccgctctc gtaggcgagg tcgtcgcggc gaccgtcgac
       61 cgccggggcg ctggcgctgc cgggcagcga catgccgagg gcctcggaga tgctggccat
      121 ggtgttggcc gtgaacatcc cggcgcaggc gccctcggtg gggcaggcgt tgcgctcgat
      181 ggcgtcgagg gtctcctggt cgatcgtgcc ggcggcgcgg gcgcccacgg cctcgaacac
      241 cgagacgatg tcgagggcct cgcccttgta ctggccgggc aggatcgagc cggcgtagag
      301 gaacacgctc ggcacgttca gccgggcggc ggccatgagc atgccgggca gcgacttgtc
      361 gcagccggcg aaggtgagca gcccgtccat gcgctcggcg tgcatgacgg tctcgacgga
      421 gtcggcgatg acctcccggg acaccagcga ggcgcgcatg ccctcatgtc ccatggagat
      481 gccgtcggac acggcgatgg tgacgaactc gatcgggtag ccgccggcgt cgcgcacgcc
      541 gaccttggag cgcttggcca ggcggtcgag gggcatgttg cacggcgtca cctcgttcca
      601 ggacgacgcc accccgatct gggccttggc gaagtcctcg tcctgcatgc cgatggcgcg
      661 cagcatggcc cgggccgggg cccgctccat cccgtcggtc acgtcacggg accgggcctt
      721 gatgtcgacg gtggtctcgg tggtgtcgtc tggtccgctc acagcgagcc aggctacgcg
      781 agtgccgatc cggagcccgc acgactcaga gatcaggagg ctggcggtgc cggcgttcgg
      841 tgcgctggtc gccgagccgc tctacgtgct ggccgacacc gccgtggtcg gccgcatcgg
      901 caccgacgag cttgccggcc tggccgtggc ctcctcggcc ctgctgaccg ggtacgccat
      961 cttcatcttc ctggcctacg gcacgacggc ggccgtgtcg cgcctgctcg gtgccggccg
     1021 ggagggctcg gccgcccacc aggccgtgca gggcctgtgg ctcgccggcg tgatcggcgt
     1081 ggtgatgacc gcgctcgtcc tcggcctcgc cgacccgatc gtggagctgc tcggcggcgc
     1141 cggagccgtg aagaccaacg cgctcgtgta cctgcggatc tcggccatcg gcatcacctt
     1201 ccagctgctc accttcgccg gcaccggcta cctgcggggc ctgcaggaca cccgcacccc
     1261 cctgctggtc gccatcggcg ccgcggtcgc gaacctggtg atcgagctgg tcctgatcaa
     1321 cgggctgggc ttcggcatcg gcgcctcggc gctctcgacg gtgatcgccc aggccggtgg
     1381 tgccctcgtc tacctgcgcc tcatcaccac ggccacgtcc cgactcgggg cggcactgcg
     1441 tccggactgg tcggccatcc gtcgcctcgg ccgggccggg tgggacctgc tggtccgcac
     1501 cctcgcgctg cggatgtcgc tcacgctgtt caccgccatc gccgcccgca tcggggccga
     1561 ggaggtcggc gcccacgaga tcgccttcgc catctggagc ctgctggccc tggccctcga
     1621 cgccatcgcc atcgccggcc aggccatggt cgg
//
LOCUS       DATISU010000405         4687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_360904, whole genome shotgun sequence.
ACCESSION   DATISU010000405 DATISU010000000
VERSION     DATISU010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360904"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VLR27_16055"
     CDS             <1..599
                     /locus_tag="VLR27_16055"
                     /inference="COORDINATES: protein motif:HMM:NF022794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
                     /protein_id="HSP05026.1"
                     /translation="TSTSTTPTTDTMTTEPDAPVVIELRTERRAMPGTEGFDQVVRDT
                     LTDPRGWRQAGFEIRFADDAPSVVLVAEGDEVDASCSPYDTGGQYSCQIGPVVALNAD
                     RWREATDSWPGTLEEYRQMLVNHEVGHLLGRHHARPACQEPGAPAAVMYQQSAGVEGC
                     APNPWPLPWEIECAARHDEPVAPPYEPDATATCGPDDV"
     gene            complement(667..1458)
                     /locus_tag="VLR27_16060"
     CDS             complement(667..1458)
                     /locus_tag="VLR27_16060"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSP05027.1"
                     /translation="MATAATVPVGDLLRGWRQRRRRSQMDLAHDVGVSPRHLSFVETG
                     RSRPSPEMVLAVARHLEVPLREQNALLLAAGYAPRYAETPLDADSMARVRTSLQRMLD
                     AHDPYPGVVIDRQWDVVLANAAALALTEGVPDHLLGPPLNVFRVCLHPDGLAARTRNL
                     PEWAGYLLHQIDRAVRLTGDHRLTEIAAEVRSYPNIAALPEAPAEVDPPLLLPVVLDM
                     GGTDVSLFTTLTTFGTPRDVTLDELAVELFFPADDAADAHLRTAR"
     gene            1567..1983
                     /locus_tag="VLR27_16065"
     CDS             1567..1983
                     /locus_tag="VLR27_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05028.1"
                     /translation="MTTIAAPPTTTTQTSVQRLRTVLSVNAATSFVAGTIGLLATDPV
                     VDELGLQSAGWTRLVSAGLVLFAIDVALGVRSARYLANTALLTSVLDLAWVVATVAVL
                     ATVELTGLGRLLAVVMGVAVLDFAVLQLWFRSRMPR"
     gene            2089..2613
                     /locus_tag="VLR27_16070"
     CDS             2089..2613
                     /locus_tag="VLR27_16070"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HSP05029.1"
                     /translation="MTDPATTQPGATESYSPLLPDDLEEHIREVLERVAEQELSLATA
                     ESCTGGMLASVLTDVQDLSGVFERGFVVYSDPAKCELLGLAQADIDRCGAVSQEVAVA
                     MAEGALANSKADIAVSTTGYADDGPEPGLVHFACARRDGPTIHREERFGAIGRGPVRI
                     GSMRVAVQMIDEVL"
     gene            2610..>4687
                     /locus_tag="VLR27_16075"
     CDS             2610..>4687
                     /locus_tag="VLR27_16075"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HSP05030.1"
                     /translation="MSSPHPFHDPATHGFVRVAAATPGVAVGDPECNAGFHLDLARRA
                     HDEGVDLVVYPELGLSSYAIDDLHLQGGMHAAVDRALRRVVEESTDLQPVLLVGLPLP
                     RNGRLYNCAAVVAHGRVLGVVPKSYLPNYREYYEKRWFAPGAGIVGAEIEAGGDTVPF
                     GTDLRFVADDHDGLDFHVEICEDYWAATPPSSIGALAGALICTNLSASNIVIGKARDR
                     ALLCASQSMRTQSAYVYSAAGPGESTTDLAWDGQGLVYELGEELAHSDRFAMEPDMVV
                     ADVDAARIRLERMRNGTFNDAAVAAGHPEDRFRVIGFEHTRRAPTDEAAPLLRPLARF
                     PFVPDDPERLEEDCYEAFNIQVEGLVTRFRAIKAEHVVIGVSGGLDSTHALIVAAKAF
                     DRMGLDRSGILGYTMPGFATSDSTKSNAWALMEALGVTGQEIDIRPAARQMLADMDHP
                     FASGEPVYDIAFENVQAGLRTDYLFRLANDNRGFVLGTGDLSELALGWCTYGVGDQMS
                     HYNVNSGVPKTLIQHLIRWTVRTDQWDPETDRILLSILDTVISPELVPSHEDADTDDP
                     ELQSTEDQIGPYELHDFFLHHIARQGLAPSKVAFLAWHAWRDAEAGRWPEGFPEDKRN
                     AYDLDVIRQWLREFLWRFFGFSQFKRSAMPNGPKVSGGGALSPRGDWRAPSDGTATPF
                     LDELERNLPPA"
BASE COUNT          672 a         1734 c         1641 g          640 t
ORIGIN      
        1 cgacctccac cagcacgacg ccgacgaccg acacgatgac cacggagccc gacgccccgg
       61 tcgtgatcga gctgcgcacc gagcgacggg ccatgccggg cacggagggg ttcgaccagg
      121 tcgtccggga cacgctcacc gacccgaggg gctggcgcca ggccgggttc gagatccgct
      181 tcgctgacga cgccccgagc gtggtgctcg tcgccgaggg cgacgaggtc gacgccagct
      241 gctcgccata cgacaccggc ggccagtact cgtgccagat cggaccggtc gtcgcgctga
      301 acgccgaccg ctggcgcgag gcgacggaca gctggccggg gacgctcgag gagtaccgcc
      361 agatgctcgt gaaccacgag gtcggccacc tcctcgggcg ccaccacgcc cgccccgcct
      421 gccaggagcc cggtgccccg gcggcggtga tgtaccagca gtccgccggg gtcgaggggt
      481 gcgcgccgaa cccgtggccc ctgccctggg agatcgagtg cgcggcccgc cacgacgagc
      541 cggtcgcccc gccctacgag cccgacgcca ccgccacgtg cggcccggac gacgtgtgac
      601 cgtcccctca ggggttctgg cctacggatg cccccgcatg cgagggaatc cgtaggccag
      661 aacggatcag cgggcggtac ggaggtgggc gtcggccgcg tcgtcggcgg ggaagaacag
      721 ctccacggcc agctcgtcga gggtgacgtc ccggggcgtc ccgaaggtcg tgagggtggt
      781 gaacagcgag acgtcggtgc cgcccatgtc gagcaccacc ggcagcagga gcggcgggtc
      841 gacctcggcg ggcgcctcgg gcagcgcggc gatgttcggg tacgaccgca cctcggcggc
      901 gatctcggtg agccggtggt cgccggtgag gcgcacggcg cggtcgatct ggtggaggag
      961 gtagccggcc cactcgggca ggttgcgggt gcgggcagcc aacccgtccg ggtgcaggca
     1021 gacccggaac acgttgagcg ggggaccgag caggtggtct ggcacgccct cggtgagggc
     1081 gagggcggcg gcgttggcca ggaccacgtc ccactgccgg tcgatcacca cgccggggta
     1141 cgggtcgtgg gcgtcgagca tgcgctgcag ggaggtgcgg acgcgggcca tcgagtcggc
     1201 gtccaggggc gtctcggcgt accggggtgc gtagccggcg gccagcagca gggcgttctg
     1261 ctcgcgcagc gggacctcga ggtggcgggc gacggccagc accatctccg ggctgggccg
     1321 ggagcggccc gtctccacga agctcaggtg gcggggggag acgccgacgt cgtgcgccag
     1381 gtccatctgg ctgcggcgcc gacgctggcg ccagccgcgg agcaggtcgc cgacggggac
     1441 ggtggcggcg gtggccatgg ccgaaccgta cggatggccg gtcatggtcg ccattacctc
     1501 agagggaatc gccgtgcctc ccggccacgc cgacgatggc gtcatccgat ccaccgagga
     1561 ggacccatga ccaccatcgc cgccccaccc accacgacca cccagacgtc ggtccagcgg
     1621 ctccgcaccg tgctgagcgt caacgccgcc acgtcgttcg tcgccggcac gatcgggctc
     1681 ctggccaccg accctgtcgt cgacgagctc ggtctgcaga gcgcgggctg gacccggctg
     1741 gtctccgccg ggctcgtgct cttcgccatc gatgtggccc tcggcgtgcg ctcggctcgc
     1801 tacctggcca acaccgccct gctcacgtcc gtgctcgacc tggcgtgggt cgtcgcgacc
     1861 gtggccgtgc tggcgaccgt cgagctgacg ggcctcggcc ggctcttggc ggtcgtgatg
     1921 ggcgtcgccg tcctggactt cgccgtcctg cagctgtggt tccggagccg catgccccgc
     1981 tgaccggccg cgggcgcgac gtcagcactg ccagttcggt gtgctcggtc accgcccatc
     2041 gacaagtcgg tggttgcgag gtcggagtga cccgggtatc ggttgcacgt gaccgatcca
     2101 gcaaccaccc agccgggtgc caccgagtcg tactccccgc tgctccccga cgacctggag
     2161 gagcacatcc gtgaggtgct cgaacgggtc gccgagcagg agctgtcact cgccacggcc
     2221 gagagctgca ccggcgggat gctcgcctcg gtgctcaccg acgtccagga cctgagcggc
     2281 gtgttcgagc ggggcttcgt cgtctacagc gacccggcca agtgcgagct gctcggactg
     2341 gcccaggccg acatcgaccg gtgcggtgcc gtcagccagg aggtggcggt ggccatggcc
     2401 gagggggccc tggccaactc caaggcggac atcgccgtgt ccaccaccgg ctacgccgac
     2461 gacggccccg agcccgggct ggtgcacttc gcatgcgccc ggcgggacgg gcccaccatc
     2521 caccgcgagg aacggttcgg cgcgatcggg cgcggaccgg tcaggatcgg gtccatgcgc
     2581 gtcgccgtcc agatgatcga cgaggtcctg tgagctcccc ccacccgttc cacgacccgg
     2641 ccacgcacgg cttcgtgcgg gtcgccgccg ccaccccggg cgtcgccgtc ggcgatcccg
     2701 agtgcaacgc cggcttccac ctcgacctgg cccgccgcgc ccacgacgag ggcgtcgacc
     2761 tcgtcgtgta ccccgagctc ggcctgtcgt cctacgccat cgacgacctc cacctgcagg
     2821 gcgggatgca cgctgccgtc gaccgggcgc tgcgtcgggt cgtcgaggag tcgaccgacc
     2881 tgcagcccgt gctgctcgtc ggcctgccgc tgccccgcaa cggtcgcctg tacaactgcg
     2941 ccgccgtcgt cgcccacgga cgggtgctcg gcgtcgtgcc gaagagctac ctgcccaact
     3001 accgcgagta ctacgagaag cgctggttcg cccccggcgc cggcatcgtc ggcgccgaga
     3061 tcgaggcggg cggcgacacg gtccccttcg gcaccgacct gcgcttcgtg gccgacgacc
     3121 acgacgggct cgacttccac gtcgagatct gcgaggacta ctgggccgcc acgccgccgt
     3181 cgtccatcgg tgccctcgca ggtgcgctga tctgcaccaa cctgtccgcg tcgaacatcg
     3241 tcatcggcaa ggcccgcgac cgggcgctgc tgtgcgcgtc gcagtcgatg cgcacgcagt
     3301 cggcctacgt gtactcggcg gccgggccgg gggagagcac caccgacctc gcctgggacg
     3361 gccagggcct cgtctacgag ctcggcgagg agctggccca ctcggaccgc ttcgccatgg
     3421 agcccgacat ggtggtcgcc gacgtcgacg ccgcccggat ccgtctggag cgcatgcgca
     3481 acggcacgtt caacgacgcc gccgtcgccg ccggccaccc cgaggaccgc ttccgggtga
     3541 tcggcttcga gcacacgcgc cgcgccccga ccgacgaagc agccccgctg ctgcggccgc
     3601 tggcccggtt cccgttcgtg cccgacgatc ccgagcgcct cgaggaggac tgctacgagg
     3661 cgttcaacat ccaggtcgag ggtctcgtca cccgcttccg cgcgatcaag gccgagcacg
     3721 tcgtcatcgg cgtgtccggt gggctcgact cgacccacgc cctcatcgtc gcggccaagg
     3781 ccttcgaccg gatgggcctc gaccgcagcg gcatcctcgg gtacacgatg cccggcttcg
     3841 ccaccagcga ctcgaccaag tccaacgcgt gggcgctcat ggaggccctc ggcgtcaccg
     3901 gccaggagat cgacatccgc ccggcggccc gccagatgct ggccgacatg gaccacccct
     3961 tcgcgagcgg cgagccggtg tacgacatcg cgttcgagaa cgtccaggcc ggcctgcgca
     4021 ccgactacct gttccgcctg gccaacgaca accgcggctt cgtgctcggc acgggcgacc
     4081 tgtccgagct ggcgctgggc tggtgcacct acggcgtcgg cgaccagatg agccactaca
     4141 acgtgaacag cggcgtgccg aagacgctca tccagcacct gattcgctgg acggtgcgca
     4201 ccgaccagtg ggaccccgag accgaccgga tcctgctgtc gatcctcgac accgtgatct
     4261 ccccggagct ggtgccgagc cacgaggacg ctgacaccga cgatcccgag ctgcagagca
     4321 ccgaggacca gatcggtccc tacgagctcc acgacttctt cctgcaccac atcgcccgcc
     4381 agggcctggc cccgtcgaag gtggccttcc tcgcctggca cgcgtggcgc gacgcggagg
     4441 ccggtcgctg gcccgagggc ttccccgagg acaagcgcaa cgcctacgac ctcgacgtca
     4501 tccgccagtg gctgcgcgag ttcctgtggc gcttcttcgg cttcagccag ttcaagcgct
     4561 cggccatgcc gaacggcccg aaggtctccg gcggcggcgc cctgtcaccc cgtggcgact
     4621 ggcgcgcacc gtcggacggg accgccacgc ccttcctcga cgagctcgaa cgcaacctgc
     4681 cgccggc
//
LOCUS       DATISU010000406         5128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_500490, whole genome shotgun sequence.
ACCESSION   DATISU010000406 DATISU010000000
VERSION     DATISU010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500490"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="VLR27_16080"
     CDS             <1..162
                     /locus_tag="VLR27_16080"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HSP05031.1"
                     /translation="DQLFYCRQRGLDEEDATSMIVNGFCREVFDELPMEYAVEAHALM
                     RLSLEGAVG"
     gene            162..911
                     /gene="sufC"
                     /locus_tag="VLR27_16085"
     CDS             162..911
                     /gene="sufC"
                     /locus_tag="VLR27_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HSP05032.1"
                     /translation="MLHIRDLHASIDGKEILAGLDLDLPAGEVHAVMGPNGAGKSTLS
                     NVLAGRDGYEVRGTATLDGTDLLALEAEDRATAGLFLAFQYPMELPGVGNAYFLRTSL
                     NAIRRAHGQDEVSATDFLAVAREHLAALEMDPAFLSRSVNEGFSGGEKKRNEILQMSM
                     LEPRLAILDETDSGLDIDALRIVARGIERLRGPDRSMLVITHYPRLLELVRPDRIHVL
                     DGGRIVRSGGHELAFELEADGYGGMTDRVPA"
     gene            908..2140
                     /locus_tag="VLR27_16090"
     CDS             908..2140
                     /locus_tag="VLR27_16090"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HSP05033.1"
                     /translation="MTAAVDDVMTEEAVHARTWLSEHGLPTGREEDWRYTPVTEVRRR
                     LDEMPDAPVPARVVTGDDVDRLAGRHGAARIVHVDGVHVPELSTDALPAGLWVGTTHN
                     LSARVRLQLTTPEGDPPDGFEALNRSASQDVLYLLVADDADLRDPVHVVHVSTGAGVS
                     HPRLVVDVGVGGRVHLVESHVSLDGGGFTNASTTVRLSARSHADVHRVQDQAADAVHV
                     GRLELHHDAGAHASVTALSRGGAISRLTTVALLREPEASCTVTALLAPGIGARHDDAV
                     TVDHLASHCTSDLTVRSVVPERSRVTSSGHAIVRPGTVATVAHQRTDSLVLHPSGQAD
                     SRPWLEIFADDVRANHGSATGRLDDDALFYLRSRGIPRAEARDVLVGAFARTLVDRID
                     PPSLRDQVAGWFGPEGTA"
     gene            2137..2691
                     /gene="sufT"
                     /locus_tag="VLR27_16095"
     CDS             2137..2691
                     /gene="sufT"
                     /locus_tag="VLR27_16095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HSP05034.1"
                     /translation="MTQTWNPTILQRDCEALTVPHGERVALTAGGEVEITQRLGSSVT
                     VRTQMGTLLRIDGADADALGLDPAPETAVHVTRQTGAFDFADVTAALRGVYDPEIPIN
                     IVDLGLVYRCDEAALEDGRRRIEIDMTMTAPGCGMGDVIARDAEAAVQRVAGVDEVDV
                     TIVWDPPWTRARISEAARLELGLF"
     gene            2806..3189
                     /locus_tag="VLR27_16100"
     CDS             2806..3189
                     /locus_tag="VLR27_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05035.1"
                     /translation="MQFRRAMALAGVLALTVSACGGDDDDAATPAGDSGSGGSELTVI
                     AEDISFPEDTYQADAGTVDVVYENEGAIEHTLLIEGVDDFKLVVESNGDVDEGSVELD
                     AGTYTIYCDVPGHQEGGMEADLEVS"
     gene            complement(3290..>5128)
                     /gene="acnA"
                     /locus_tag="VLR27_16105"
     CDS             complement(3290..>5128)
                     /gene="acnA"
                     /locus_tag="VLR27_16105"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HSP05036.1"
                     /translation="VEAYAKAQGLWHDPTATARYSEHLSLDLSTVVPSLAGPARPQDR
                     VPLDRSKAMFADALADIADRRGAGGGPKPGVPSQEQPVEVRTGVDEASAESFPASDPP
                     ALDGDEPAPEPVPAPRDHDGHEHPPSPRVTVEREDGAFELTDGSVVIAAITSCTNTSN
                     PSVMVAAGLLAKKAVERGLRTKPWVKTTLAPGSQVVTDYFERAGLTPYLDELGYQLVG
                     YGCTTCIGNSGPLAPDISAAIGEGDLAAVSVLSGNRNFEGRIHPEVRMNYLASPPLVV
                     AYGLAGRMDVDLFAEPLGTGHDSEPVHLRDIWPTPTEVQEVIDAALETEMFTRRYAEV
                     YEGDERWRALPLSSGDRYEWSGDSTYIRQPTFFDGMGPEPSPITDVEGARVLALLGDS
                     VTTDHISPAGAIKADGPAGRWLQDQGVPVEEFNSYGSRRGNHEVMVRGTFANIRLRNQ
                     LAPGTEGGVTVHQPSGEQTTIYDAAERYRRDGVPLVVLAGAEYGSGSSRDWAAKGTLL
                     LGVRAVIATSFERIHRSNLIGMGVLPLELVGGEDVSSLGLTGREVVDIRGLDGRSEQD
                     LIGATLSVDADGTTFEVRTRIDTPMEAAYYRHGGILQYVLRDLAGS"
BASE COUNT          733 a         1899 c         1800 g          696 t
ORIGIN      
        1 gaccagctct tctactgccg ccagcgaggc ctcgacgagg aggacgccac gtccatgatc
       61 gtcaacggct tctgccggga ggtcttcgac gagctgccca tggagtacgc ggtcgaggcc
      121 cacgccttga tgcgcctgag cttggaagga gcggtgggct gatgctgcac atccgcgacc
      181 tgcacgcatc gatcgacggc aaggagatcc tcgccggact cgatctcgac ctccccgccg
      241 gcgaggtcca cgccgtcatg ggacccaacg gcgccggcaa gagcacgttg tcgaacgtgc
      301 tcgccggccg cgacggctac gaggtccggg ggacggcgac cctcgacggc accgatctgc
      361 tggccctcga agccgaggac cgcgccaccg ccggtctctt cctcgccttc cagtacccga
      421 tggagctccc cggcgtcggg aacgcctact tcctgcgcac gtcgctcaac gccatccgac
      481 gggcccacgg ccaggacgag gtcagcgcga ccgacttcct cgccgtcgcc cgggagcacc
      541 tcgcggcgct cgagatggac ccggcgttcc tcagccggtc ggtcaacgag ggcttctccg
      601 gcggggagaa gaagcggaac gagatcctgc agatgtcgat gctcgaacct cgcctcgcca
      661 tcctcgacga gaccgactcc ggcctcgaca tcgacgcgct gcgcatcgtc gcccggggca
      721 tcgaacgcct gcggggacca gaccgctcga tgctcgtcat cacccactac ccccgcctgc
      781 tggagctggt ccggcccgac cgcatccacg tcctcgacgg cgggcgcatc gtccgctccg
      841 gcggccacga gctggccttc gagctcgagg cggacgggta cggcgggatg accgatcggg
      901 tgccggcatg acagccgccg tcgacgacgt gatgaccgag gaagccgtcc acgcccggac
      961 gtggctgtcc gagcacgggc tccccaccgg gcgcgaggag gactggcgct acacgccggt
     1021 caccgaggtc cgccgtcgcc tcgacgagat gcccgacgca ccggtgccgg cgcgggtcgt
     1081 caccggagac gacgtcgacc gcctcgccgg gcggcacggc gcggcgcgca tcgtgcacgt
     1141 ggacggcgtg cacgtcccgg agctgtcgac cgatgccctc ccggccggcc tctgggtcgg
     1201 gaccacgcac aacctgtccg cccgcgtccg gctccagctg acgacgccgg agggcgatcc
     1261 gcccgatggc ttcgaggcgc tgaaccgctc ggcatcgcag gacgtcctct acctgctcgt
     1321 ggccgacgac gccgacctcc gggatccggt ccacgtcgtg cacgtcagca ccggcgccgg
     1381 cgtgtcccac ccccgcctcg tcgtcgacgt cggcgtcggc ggtcgcgtcc acctcgtcga
     1441 gagccacgtg agcctggacg gcggcggctt cacgaacgcc tccaccaccg tccggctttc
     1501 ggcccggtcg cacgcggacg tgcaccgggt ccaggaccag gccgccgacg cggtgcacgt
     1561 cggccgcctc gagctccacc acgacgccgg tgcgcacgcc tcggtcaccg cgctgtcgcg
     1621 aggtggcgcc atctcgcgcc tgacgacggt cgcgctgctc cgggagccgg aggcatcgtg
     1681 cacggtcacc gcgctgctcg ctcccgggat cggggcgcgc cacgacgacg ccgtcaccgt
     1741 cgatcacctc gcctcccact gcacgagcga cctcaccgtg cgcagcgtcg tgccggagcg
     1801 atcccgggtg acctcgagcg gacacgccat cgtgcgcccc ggcacggtcg ccacggtcgc
     1861 ccaccagcgc accgacagcc tggtgctcca cccgtcgggc caggcggaca gccggccgtg
     1921 gctggagatc ttcgccgacg acgtgcgggc gaaccacggg tcggccaccg ggcgactcga
     1981 cgacgacgcg ctcttctacc tccgcagccg gggcatcccc cgcgccgagg cccgagacgt
     2041 gctggtcggc gccttcgccc gcaccctcgt cgaccgcatc gacccgccat cgctccgcga
     2101 ccaggtcgcg gggtggttcg ggccggaggg aacggcatga cccagacctg gaaccccacc
     2161 atcctccagc gcgactgcga ggcgctcacc gtgccccacg gcgagcgtgt cgcgctcacg
     2221 gccggcggcg aggtcgagat cacccaacgg ctcgggtcga gcgtcaccgt ccggacccag
     2281 atgggcacgc tgctgcgcat cgacggcgcc gacgccgacg ccctcggcct cgatcccgcg
     2341 cccgagaccg cggtccacgt cacccgccag acgggggcgt tcgacttcgc cgacgtcacg
     2401 gccgccctgc ggggcgtcta cgacccggag atcccgatca acatcgtcga cctcggcctg
     2461 gtgtaccgct gcgacgaggc ggcactcgag gacggccggc gtcgcatcga gatcgacatg
     2521 acgatgaccg cacccggctg cggcatgggc gacgtcatcg cccgtgacgc cgaagccgcc
     2581 gtccagcgcg tcgccggcgt cgacgaggtg gacgtgacga tcgtgtggga cccgccctgg
     2641 acccgggccc ggatctccga ggcggcccgg ctggagctgg ggctcttctg atcccggcgt
     2701 cccctccgag cggggcgacg tccacccgcc acgccgtgcg agcagcatga cgacgacgac
     2761 caccaccaca acgacaacga ccacgagaac cacggaggtt cgatgatgca gttccgacga
     2821 gcgatggccc tggccggcgt gctggccctg acggtgagcg cctgcggtgg cgatgacgac
     2881 gacgccgcca ccccggcggg tgacagcggt tccgggggga gcgagctcac cgtgatcgcg
     2941 gaggacatct cgttccccga ggacacctac caggccgacg ccggcaccgt cgacgtggtg
     3001 tacgagaacg agggcgccat cgagcacacc ctcctcatcg agggcgtgga cgacttcaag
     3061 ctggtcgtgg aatccaacgg cgacgtcgac gagggcagcg tcgagctcga cgccggcacc
     3121 tacaccatct actgcgacgt gccgggccac caggagggcg gcatggaagc ggacctcgag
     3181 gtcagctgag cgaccccttg gcctgacctg ctcgttctgg gccgctcacc acgcttccca
     3241 tgcgtgtacg acgtcccagt tcggtgggcg gcaccgtccc agggggcact cacgacccgg
     3301 cgaggtcgcg cagcacgtac tggaggatgc cgccgtggcg gtagtaggcg gcctccatcg
     3361 gggtgtcgat gcgggtgcgc acctcgaagg tggtgccgtc ggcgtcgacg gacagcgtcg
     3421 ccccgatcag gtcctgctcc gatcgtccgt cgaggccgcg gatgtcgacg acctcccggc
     3481 cggtgaggcc gagcgacgag acgtcctcgc cacccacgag ctcgagcggc agcaccccca
     3541 tgccgatcag gttcgaccgg tggatgcgct cgaaggacgt ggcgatcacg gcgcggacgc
     3601 cgagcagcag cgtgcccttg gccgcccagt cgcgcgacga ccccgagccg tactcggcac
     3661 cggccagcac gacgagcggc acgccgtcgc ggcggtagcg ctcggcggcg tcgtagatgg
     3721 tcgtctgctc gccgctcggc tggtggacgg tgacaccgcc ctccgtgccc ggcgccagct
     3781 ggttgcggag ccggatgttg gcgaacgtgc cgcgcaccat cacctcgtgg ttgccgcggc
     3841 gggagccgta ggagttgaac tcctcgaccg gcacgccctg gtcctgcagc cagcgaccgg
     3901 ccggtccgtc ggccttgatg gcgccagcgg gggagatgtg gtcggtggtc acgctgtcgc
     3961 cgagcagcgc gaggacgcgg gcgccctcga cgtcggtgat cggcgacggc tccggcccca
     4021 tcccgtcgaa gaaggtcggc tggcggatgt aggtcgagtc cccgctccac tcgtagcggt
     4081 cgccgctcga cagcggcagg gcccgccacc gctcgtcgcc ctcgtagacc tcggcgtagc
     4141 gccgggtgaa catctcggtc tccagcgcgg cgtcgatcac ctcctgcacc tcggtcggcg
     4201 tcggccagat gtcgcgcagg tggaccggct cgctgtcgtg gccggtgccg aggggctcgg
     4261 cgaacaggtc gacgtccatc cgaccggcga ggccgtaggc cacgaccagc ggcggtgagg
     4321 ccaggtagtt catccgcacc tcggggtgga tgcggccctc gaagttgcgg ttgccggaca
     4381 gcaccgacac cgccgccagg tcgccttcac cgatggcggc ggagatgtcc ggcgccagcg
     4441 gccccgagtt gccgatgcag gtggtgcagc cgtagcccac gagctggtag ccgagctcgt
     4501 cgaggtaggg ggtgagcccg gcccgctcga agtagtcggt gaccacctgg gacccgggtg
     4561 cgagcgtggt cttcacccac ggcttggtcc gcaggccgcg ctccaccgcc ttcttggcca
     4621 gcaggccggc agcgaccatc accgacgggt tcgaggtgtt cgtgcagctg gtgatggcgg
     4681 cgatgaccac ggacccgtcg gtgagctcga acgcgccgtc ctcccgttcg accgtcacgc
     4741 gtggggacgg cgggtgctcg tggccgtcgt ggtccctcgg tgccggcacc ggctccggag
     4801 cgggctcgtc gccgtcgagc gccggagggt cgctggccgg gaacgactcg gccgaggcct
     4861 cgtccacgcc ggtgcgcacc tcgaccggct gctcctggga cggcacgccc ggcttcggac
     4921 cgccgcccgc gccacgacgg tcggcgatgt cggcgagggc gtcggcgaac atcgccttcg
     4981 agcggtcgag gggcacgcgg tcctgcggcc gtgccgggcc ggcgaggctc ggcaccacgg
     5041 tcgacaggtc gagggacagg tgctcggagt accgggcggt ggcggtcggg tcgtgccaca
     5101 ggccctgcgc cttcgcgtag gcctccac
//
LOCUS       DATISU010000407         1944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_593995, whole genome shotgun sequence.
ACCESSION   DATISU010000407 DATISU010000000
VERSION     DATISU010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593995"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VLR27_16110"
     CDS             complement(<1..635)
                     /locus_tag="VLR27_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HSP05037.1"
                     /translation="MPRPGLVLDVDSSTPPIVFHHGEDFVLEKLPAGRSRVIYPPEPH
                     EGLKDTDAAIRHALENPLGDSQPLSSLLFSGMKLTICFDDISLPLPPMRKPDVRQRII
                     EAVLDTAAAAGVDDVEIIAALALHRRMTEDELRHAVGDRVYDAFAPSGRLYNHDAEDP
                     DGMLEIGTTDKGELVEINKRAATSDLIVYVNINLVSMDGGWKSTATGLSGYR"
     gene            complement(636..1838)
                     /locus_tag="VLR27_16115"
     CDS             complement(636..1838)
                     /locus_tag="VLR27_16115"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSP05038.1"
                     /translation="MKALEFSRKPARYAAAMIAGALRPGTGAAVGPLRLRDLDEPEAP
                     ADGWVTLRPRLAGICGSDLSTIDGHSSRYFEPIVSFPFVLGHEVVGDLEDGTRAVLVP
                     VLHCEVRGITPPCPGCASGDVNRCERIAFGHLEPGLQSGFCESTGGGWSEAMVAHPAQ
                     LVPVPDDLSDEAAVMVEPTACAVHAADVAVGALQAVDGDGPVVVIGAGTLGLLTIAAM
                     SDALPTGMPIITGARYPHQKRLATELGATTVVSPDELNGAVRLATRSMKLGADHAAGQ
                     LTGGASVVVDCVGSEASLQQALEVVAPGGSILVVGMPATVSLDLTGLWHREVALRGVY
                     AYRREDFDTAIRLVSPTGPHGDLGRLVSATYPLSRYEDAIVHAADAGRRGAVKIAFDL
                     RKTSERKP"
BASE COUNT          273 a          616 c          773 g          282 t
ORIGIN      
        1 cggtagccgg acaggccggt ggcggtggac ttccagccgc cgtccatcga cacgaggttg
       61 atgttcacgt agacgatgag gtccgacgtg gcggcccgct tgttgatctc gaccagctcg
      121 cccttgtcgg tggtgccgat ctcgagcatc ccgtcggggt cctcggcgtc gtggttgtac
      181 agccggccgg acggcgcgaa ggcgtcgtac acgcggtcgc cgacggcgtg gcgcagctcg
      241 tcctcggtca tgcgccggtg gagggcgagg gcggcgatga tctcgacatc gtccaccccg
      301 gcggccgcgg cggtgtcgag gacggcctcg atgatccgct ggcgcacgtc cggcttgcgc
      361 atcgggggca gcggcaggga gatgtcgtcg aagcagatcg tcagcttcat gcccgagaag
      421 agcaggctgg agagcggctg gctgtcgccg agtgggttct cgagcgcgtg ccggatggcg
      481 gcgtcggtgt ccttcaggcc ctcgtggggt tcgggcgggt agatcacccg gctccggccg
      541 gcgggcagct tctcgagcac gaagtcctca ccgtggtgga acacgatggg tggggtcgac
      601 gagtcgacgt cgaggacgag tccggggcga ggcatctagg gcttccgttc tgatgtcttg
      661 cggaggtcaa atgcgatctt gacggcgccg cggcggccgg cgtcagcggc gtggacgatg
      721 gcgtcctcgt agcgggacag ggggtaggtg gcgctcacca agcgcccgag gtcgccgtgg
      781 ggacccgtgg gcgacacgag gcggatggcc gtgtcgaagt cctcacggcg gtaggcgtag
      841 acgccgcgga gcgcgacctc gcggtgccac aggccggtga ggtcgaggga gacggtggcg
      901 ggcatgccga ccacgaggat tgagccgccg ggggcgacga cctcgagggc ctgctggagg
      961 ctcgcctcgc tgccgacgca gtcgacgacc accgatgcgc cgccggtcag ctggccagca
     1021 gcgtgatcgg cgccgagctt catggatcgg gtggcgagcc ggacggcgcc gttgagctcg
     1081 tcgggcgaca cgaccgtcgt ggcgccgagc tcggtggcca gccgcttctg gtgcgggtag
     1141 cgggcgccgg tgatgatcgg catgcccgtc ggcagggcgt cgctcatggc agcgatggtg
     1201 agcaggccga gggtgccggc gccgatgacc accacgggtc cgtcgccgtc gacggcctgg
     1261 agtgcaccaa cggcgacatc ggccgcgtgg accgcgcagg ccgtgggctc gaccatgacg
     1321 gcggcttcgt cgctgaggtc gtcgggcacc gggacgagct gggcggggtg ggcgaccatc
     1381 gcctcggacc aaccgccgcc ggtgctctcg cagaagccgg actgcagacc gggctcgagg
     1441 tgaccgaacg cgatgcgctc gcagcggttc acgtcgcccg acgcgcagcc agggcagggg
     1501 ggagtgatgc cgcggacctc gcagtggagc accgggacca gcacggctcg cgtgccgtcc
     1561 tccagatcac cgacgacctc gtgcccgagc acgaacggga agctcacgat cggttcgaag
     1621 tagcgcgagg agtggccgtc gatggtcgac aggtccgagc cgcagatgcc agcgaggcgg
     1681 ggccgcagcg tgacccagcc gtcggcgggc gcctcgggct cgtcgaggtc gcgcaggcgg
     1741 agcggtccga ccgcggcgcc ggtgccggga cgcagggcgc cggcgatcat cgctgccgcg
     1801 taccgggccg gcttgcggga gaactcgagc gccttcatcg cgcgctcgcg gtcgtctggt
     1861 tccaactcgg cccgatgggg agcaaggggt ggctgccgcc cttggccttg tcgaagtgct
     1921 cgacgagcca gccccgcttg cgag
//
LOCUS       DATISU010000408         5922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_826366, whole genome shotgun sequence.
ACCESSION   DATISU010000408 DATISU010000000
VERSION     DATISU010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826366"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VLR27_16120"
     CDS             <1..415
                     /locus_tag="VLR27_16120"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSP05039.1"
                     /translation="ETMLMRDVDQVDRTVSALRHAGVSLAIDDFGTGYSSMSQLHRLD
                     VTACKIDRGFVAGAPEHPRDAAILRALVDVGTAFGFPVVAEGVERPEELSAVQASGCP
                     LVQGFLLAVPSPPEALTEMLLSGSVTLPGAVPSAR"
     gene            412..969
                     /locus_tag="VLR27_16125"
     CDS             412..969
                     /locus_tag="VLR27_16125"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HSP05040.1"
                     /translation="MTARIRPTHPRLDPLGDDEIDDETRALVPAGSLNIFRTLARHPK
                     LLKRWLVFGNHVLAKSTLPERERELAILRVGWRCGAEYEFGQHTVIARRCGITDDEIV
                     ALAGELADGGWSEADRTLLVAVDELHDDQCITEQTWSALAQRWSDQQLLDLIFTVGQY
                     TLVSMALNSVGVPLDDGVPGFPDRG"
     gene            962..1765
                     /locus_tag="VLR27_16130"
     CDS             962..1765
                     /locus_tag="VLR27_16130"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSP05041.1"
                     /translation="MAEGRLAGKVALVVGGGQTPGETTGNGRAISCRFAAEGASVVVV
                     DRVLARAEETVSIIEAGGGEAIAVEGDITSSDDCAAMVAATVEAHGRMDVVVDNVGIG
                     GNDGGPVGLDEAVWDRIQDVNLKGAYLLCKHALPVLREQGSGSIVLVSSVAAICSTGM
                     LAYTTSKAALNALCHEVAMGNATRGIRCNAVMPGLMDTPMAVDALAGALGVDRAVVSE
                     RRDAMVPLGGRQGTADDVANAVLFLASDEARFVTAAVLPVDGGQSARIG"
     gene            complement(1829..2893)
                     /locus_tag="VLR27_16135"
     CDS             complement(1829..2893)
                     /locus_tag="VLR27_16135"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HSP05042.1"
                     /translation="MTGRSINLFSDTQTRPTEAMRAAMAAAEVGDEQRKADPTVNRLQ
                     DRMAELLGKERALFFPTGTMCNLVAIGAHTRHGDSIVLEHSGHVLRSETSGAAAVNGC
                     VIETIRGDRGFFTPDQLAQVLTPGNQYVPPTRLVCLEQTHNFAGGTVWPLEQYRAVAA
                     LAHQQDVAVHTDGARLMNAVVASGVPAADWAAPVDTIWVDFTKGLGAPTGAVLAGPAD
                     VMEQAWLLKHRFGGAMRQAGIVAAGCLHALDHHVDRLADDHANAARLAEGLTGLGLDV
                     TAPETNLVYVDPRPAGLDAASFAAAMDEHGVEISTIGDRNRLCTHLDVTEADVDEALT
                     IAARVVEAGATGLAEGERYA"
     gene            complement(2920..3816)
                     /locus_tag="VLR27_16140"
     CDS             complement(2920..3816)
                     /locus_tag="VLR27_16140"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HSP05043.1"
                     /translation="MTDERTTTDRLRRALAARAETIHPEPDGLDRIEERLMARTPLTD
                     TQRWTIAGVAAVATLLIVTLFVVSAGEDDDDTDVATDTTTTTSAPEETTTTTTTEPEE
                     TTTTAPFAPAADPFSVAFPSPLDSRSFEAPPAAARAYATDVLGFTELVLGGPTTTGEG
                     AAEVAIQDREDGPTTTVFLEQRDDGAWYVIGSETEDIVVTAPGPGTSLATPFETSGEA
                     LAFEGTVEVVVLAQGDPGPLGQGVVTGSGTPPAGPFSGTIEFRSPSEPTAGVLVYRVR
                     SPEDGRVVQATSLRVRLTPFTS"
     gene            complement(3813..4316)
                     /locus_tag="VLR27_16145"
     CDS             complement(3813..4316)
                     /locus_tag="VLR27_16145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP05044.1"
                     /translation="MAPPLRRAEPWLVALYRAHHRDLVRLATLLVDDQGSAEEAVQDA
                     YVSLARRGPAGVDDPEAYLRTAVLNNARSALRKRRVRRRHLRSVERDRSAPGADHAVL
                     LDDDARRVLAALDQLSERQREVLVLRYWADLSEAEIATALGISAGSVKTHAHRGTAAL
                     AQILEAP"
     gene            4413..5000
                     /locus_tag="VLR27_16150"
     CDS             4413..5000
                     /locus_tag="VLR27_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05045.1"
                     /translation="MELLRGGCVVCREPGPALCTSCLRRTEPPPSTRMRELGTVHALF
                     AYEGHGARIVQALKYRDGRRLVAPLADELAGRWAEVAPAAPGLIVTWVPTSGRRRRDR
                     GFDQSELLARALARRLGATARALLRRRPGPAQTGLDRAAREAAAGYRATARAGGPVLL
                     LDDVCTTGSTLRAASAALAGVGTAPIGHLVLARTP"
     gene            5079..5777
                     /locus_tag="VLR27_16155"
     CDS             5079..5777
                     /locus_tag="VLR27_16155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSP05046.1"
                     /translation="MTEWDGDDPASDDIRVMICDDHALFRRGLIMVLEAEDDIEVVAE
                     AEDGEDALEKAVEFVPDVVLMDVRMPGVDGIEATRRIAEKVPTAKILMLTVSDEEEDL
                     YEAIKAGATGYLLKEVSIEEVAPAARAVVAGQSLISPSMASKLLGEFSNLAKRAEERS
                     SVPTPRLTERELEVLRLVAQGKSNREIAGDLYISENTVKNHVRNILEKLHLHTRMEAV
                     MYAVREKLLEIPGT"
BASE COUNT          766 a         2098 c         2244 g          814 t
ORIGIN      
        1 cgagaccatg ctgatgcgcg acgtggacca ggtcgaccgc accgtgtccg ccctgcgcca
       61 cgccggggtc agcctcgcca tcgacgactt cggcaccggc tactcctcga tgagccagct
      121 gcaccgcctc gacgtgaccg cttgcaagat cgatcgcggg ttcgtggccg gggcgccgga
      181 gcacccccgc gacgccgcca tcctgcgcgc cctggtcgac gtcggcaccg ccttcgggtt
      241 cccggtcgtg gccgagggcg tcgagcgccc ggaggagctg tcggccgtgc aggccagcgg
      301 ctgcccgctc gtgcagggct tcctgctggc cgtcccgtcg ccacccgagg ccctgaccga
      361 gatgctcctg tccggttcgg tgaccctgcc gggagcagta ccttcggccc gatgacggcc
      421 aggatccgcc ccacccaccc gcggctcgac ccgctcggcg acgacgagat cgacgacgag
      481 acccgggcgc tcgtaccggc gggttcgctg aacatcttcc gcacgctggc ccgccacccc
      541 aagctgctga agcggtggct ggtcttcggg aaccacgtgc tggccaagag caccctgccc
      601 gagcgcgagc gggagctggc catcctgcgg gtgggctggc ggtgcggcgc cgagtacgag
      661 ttcggccagc acaccgtcat cgcccggcgc tgcggcatca ccgacgacga gatcgtcgcc
      721 ctcgccggcg agctggccga cgggggctgg agcgaggcgg accgcaccct gctcgtcgcc
      781 gtcgacgagc tccacgacga ccagtgcatc accgagcaga cctggtcggc actggcccag
      841 cggtggagcg accagcagct gctcgacctg atcttcaccg tcggccagta cacgctggtc
      901 tcgatggcgc tcaacagcgt cggcgtaccg ctcgacgacg gcgtccccgg cttccccgac
      961 cgtggctgaa ggcaggctcg ccggaaaggt cgccctggtc gtgggcggcg gccagacccc
     1021 gggcgagacc accgggaacg gccgggccat ctcgtgccgg ttcgccgccg agggcgcatc
     1081 ggtggtcgtc gtcgatcggg tgctggcccg tgccgaggaa acggtctcga tcatcgaggc
     1141 cggcggtggc gaggccatcg cggtggaagg cgacatcacc tcgagcgacg actgcgcggc
     1201 gatggtcgct gccacggtcg aggcccacgg ccggatggac gtcgtggtcg acaacgtcgg
     1261 catcggcggc aacgacggcg ggccggtcgg gctcgacgag gcggtctggg accgcatcca
     1321 ggacgtcaac ctgaagggcg cgtacctgct ctgcaagcac gccctgccgg tgctgcgcga
     1381 gcaggggtcc gggtccatcg tgctggtgtc gtcggtggcc gcgatctgct ccaccgggat
     1441 gctcgcctac accacgtcga aggcggcgct gaacgccctg tgccacgagg tcgcgatggg
     1501 caacgccacg cgcggcatcc gctgcaacgc ggtgatgccg gggctgatgg acacacccat
     1561 ggccgtcgac gccctcgccg gcgcgctcgg tgtcgaccgg gccgtcgtgt ccgagcgccg
     1621 cgacgcgatg gtgccgctcg gtggacggca ggggacggcc gacgacgtcg ccaacgccgt
     1681 gctcttcctc gcctccgacg aggcccgctt cgtcaccgcc gccgtcctcc cggtcgacgg
     1741 cggccagtcc gcccgcatcg gctgagaccc gagccgttct ggcctacatc cgtgccagct
     1801 ggtggtcatc gatgtaggcc agaacggatc aggcgtagcg ctcgccctcg gccaggccgg
     1861 tggcgccggc ctccacgacc cgggcggcga tggtgagggc ctcgtccacg tcggcttcgg
     1921 tcacgtccag gtgggtgcag aggcggttgc ggtcgccgat ggtgctgatc tccacgccgt
     1981 gctcgtccat cgccgccgcg aacgacgcgg cgtcgaggcc ggccgggcgg gggtcgacgt
     2041 agacgaggtt ggtctcgggg gcggtcacgt cgagaccgag cccggtgagg ccctcggcca
     2101 gccgggcggc gttggcgtgg tcgtcggcga ggcggtcgac gtggtggtcg agggcgtgca
     2161 ggcacccggc cgccacgatg ccggcctggc gcatggcgcc gccgaagcgg tgcttgagca
     2221 gccacgcctg ctccatgacg tcggccggac cggccagcac ggcgcccgtc ggggcgccga
     2281 ggcccttggt gaagtcgacc cagatcgtgt cgaccggtgc ggcccagtcg gccgcgggca
     2341 cgccggaggc gaccaccgcg ttcatgagac gggcgccgtc ggtgtgcacg gccacgtcct
     2401 gctggtgggc gagagcagcc acggcccggt actgctcgag gggccagacc gtgccgccgg
     2461 cgaagttgtg cgtctgctcc aggcacacca accgagtggg cggcacgtac tggttgccgg
     2521 gggtgagcac ctgggcgagc tggtcgggcg tgaagaagcc acggtcgccg cggatggtct
     2581 cgatgacgca cccgttcacg gcggcggcgc ccgaggtctc ggaccgcagc acgtggccgc
     2641 tgtgctcgag cacgatcgag tcgccgtggc gggtgtgggc gccgatcgcc accaggttgc
     2701 acatggtgcc ggtggggaag aagagcgccc gctccttgcc gaggagctcg gccatgcggt
     2761 cctgcaggcg gttgaccgtc gggtcggcct tgcgctgctc gtcgcccacc tcggcggcgg
     2821 ccatggcggc gcgcatcgcc tcggtggggc gggtctgggt gtcggagaag aggttgatcg
     2881 atcgaccggt cacccgggca gcctgccagg gaccggtcgc tacgaggtga agggggtgag
     2941 gcgcacccgc agcgaggtgg cctgcacgac ccgaccgtcc tcgggggagc gcacccggta
     3001 gacgagcacg ccggcggtgg gctccgacgg cgaccggaac tcgatggtgc cgctgaacgg
     3061 ccccgccggc ggcgtcccgc tgccggtgac gaccccctga ccgagcgggc cggggtcgcc
     3121 ctgggcgagc acgacgacct cgacggtgcc ctcgaaggcg agggcctcgc cgctggtctc
     3181 gaacggtgtg gcgagcgacg tgccggggcc gggcgcggtc acgacgatgt cctcggtctc
     3241 ggagccgatc acgtaccagg caccgtcgtc gcgctgctcg aggaacacgg tcgtcgtcgg
     3301 gccgtcctcg cggtcctgga tggccacctc cgccgcgccc tcgcccgtgg tggtggggcc
     3361 gccgagcacc agctcggtga agccgaggac atcggtcgcg taggcccggg ccgccgccgg
     3421 tggggcttcg aagctgcggc tgtccagcgg cgacgggaac gccacgctga acgggtcggc
     3481 cgccggcgcg aacggcgccg tcgtcgtggt ctcctccggt tcggtggtcg tcgtcgtggt
     3541 ggtctcctcc ggcgccgacg tggtggtcgt ggtgtcggtg gccacgtcgg tgtcgtcgtc
     3601 gtcctcgccg gcggacacga cgaagagggt gacgatgagc agcgtggcca cggcggccac
     3661 gccggcgatc gtccagcgct gggtgtcggt gagtggggtg cgggccatga gtcgctcctc
     3721 gatgcggtcg agcccgtcgg gctcggggtg gatggtctcg gcgcgggcgg ccagggcccg
     3781 gcgcaggcgg tcggtggtcg ttcgctcgtc ggtcacgggg cctccaggat ctgggccagg
     3841 gcggccgtcc cgcggtgggc gtgggtcttc acggagccgg ccgagatgcc gagcgcggtg
     3901 gcgatctcgg cctcggacag atcggcccag tagcgcagca ccaggacctc ccgctggcgc
     3961 tcgctcagct ggtcgagggc ggcgaggacc cggcgggcgt cgtcgtcgag cagcacggcg
     4021 tggtcggcgc ccggcgcaga gcggtcgcgc tcgacgctgc gcaggtggcg gcggcgcacg
     4081 cgccgcttgc ggagggccga ccgggcgttg ttgagcacgg cggtgcgcag gtaggcctcg
     4141 gggtcgtcca caccggcggg tccccgccgg gccagggaca cgtaggcgtc ctgcaccgcc
     4201 tcctcggcgc tgccctggtc gtccacgagg agggtggcca ggcgcaccag gtcgcggtgg
     4261 tgcgcccggt acagcgccac gagccagggt tcggcccgcc gcaggggagg tgccatggtg
     4321 gccatcatca cctccgagga cgtccgacga gcggatgggt tgacgcgatc gagcaggttt
     4381 cctgtcgggg ggtcgcacct accgtgccgc ccgtggagct gctgcgaggg gggtgcgtcg
     4441 tctgtcggga gccgggcccg gcgctgtgca catcgtgcct gcgccgcacc gagccacccc
     4501 cgtccacccg catgcgcgag ctcggcacgg tgcacgccct cttcgcctac gagggccacg
     4561 gcgcccgcat cgtccaggcg ctgaagtacc gggacggccg gcggctggtg gcccccctcg
     4621 ccgacgagct ggccggccgg tgggccgagg tcgcgcctgc cgcgccgggg ctgatcgtca
     4681 cctgggtgcc gacctcgggt cggcgccggc gggaccgggg gttcgaccag tccgagctgc
     4741 tcgcccgcgc cctggcccgc cgcctcggcg ccaccgctcg cgcgctgctg cgccgccgac
     4801 cgggcccggc ccagacgggc ctcgaccggg ccgcccgcga ggcggccgcg ggctaccgcg
     4861 ccaccgcccg ggcgggtggc cccgtgctgc tcctcgacga cgtctgcacc accggatcca
     4921 cgctgcgtgc cgcatccgcc gcgctcgccg gtgtgggcac cgcacccatc gggcacctgg
     4981 tcctggcccg gaccccctga cccgtccggg cggctcccac ccacccggac gggcggagtc
     5041 ggtaccatgc gcggcctagc acgttcggag gatcagcgat gactgaatgg gacggggacg
     5101 acccggcgag cgatgacatc cgggtgatga tctgtgatga tcacgcgctg ttccgccgcg
     5161 ggttgatcat ggtcctcgag gccgaggacg acatcgaggt cgtcgccgag gccgaggacg
     5221 gggaggacgc gctggagaag gccgtcgagt tcgtgcccga cgtggtgctg atggacgtgc
     5281 ggatgcccgg cgtcgacggc atcgaggcca cccgccgcat cgccgagaag gtccccaccg
     5341 ccaagatcct catgctcacg gtcagcgacg aggaggagga cctctacgag gccatcaagg
     5401 cgggcgccac cggctacctg ctgaaggagg tctcgatcga ggaggtcgcg ccggcggcgc
     5461 gggccgtcgt cgccggccag agcctcatca gcccgtcgat ggcctccaag ctgctcggcg
     5521 agttctccaa cctggccaag cgggccgagg agcgcagctc ggtgcccacg ccgcggctca
     5581 ccgagcgcga gctcgaggtc ctgcgcctgg tcgcccaggg caagtccaac cgcgagatcg
     5641 ccggcgacct gtacatctcg gagaacacgg tcaagaacca cgtgcgcaac atcctcgaga
     5701 agctgcacct gcacacccgc atggaggcgg tcatgtacgc cgtgcgcgag aagctcctgg
     5761 agatccccgg cacctgacgg ccccgggtgg cgtcgtcgct acccgccggc ggcggccacg
     5821 acgaggtcgc ggtagacctc ctggccccgg cgggtcaggt ggaccccgtc gtcgaccagc
     5881 aggcccggct cgctggcgga cgctgcgtac cagtcggcga gc
//
LOCUS       DATISU010000409         3277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_454605, whole genome shotgun sequence.
ACCESSION   DATISU010000409 DATISU010000000
VERSION     DATISU010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454605"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VLR27_16160"
     CDS             complement(<1..495)
                     /locus_tag="VLR27_16160"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSP05047.1"
                     /translation="MPPARQRPTVRQLEYVVAVADHLSFSAAAETCGVSQPALSTQVR
                     EVEQRLGLSLFERGRGGVSVPDHAVAVIDAARRAIRAVDDVADAAVDLQGELVGPVRI
                     GIIPTMAPYLLPTLVRELRRRHPRAEPVLTEERTDDLVRRIELGELDLGLLAEPVPSE
                     TLASK"
     gene            608..2146
                     /locus_tag="VLR27_16165"
     CDS             608..2146
                     /locus_tag="VLR27_16165"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HSP05048.1"
                     /translation="MTAPHDPLEHLRAATSRALAGNLETQAAKLEKQGKLFVRDRLDL
                     LLDAGSFVEDALLANNQGEDLPADGVVTGTGTIDGRRVCVVANDPTVKAGSWGARTVE
                     KMIRTSEYALAHDLPIFWLVDSAGARITDQVQLFPGRRGAGRIFANQIRLSGRVPQIC
                     CLFGPSAAGGAYIPSFCDVVFMVEGNASMYLGSPRMAEKVIGEHTTLEEMGGARMHAS
                     VSGCGDALAHDDEDALDQARAWFSYFPQTWREQPPRYEPVPPAHDLADDVVPVEEAVA
                     FDMHAFIDGLVDAESFFEYKPLFAQELICGLGRIDGRPVGVVANNPSHLGGVLFVDSA
                     DKASRFVWLCDAFNIPLLFLADVPGFMIGTQVERQGIIRHGAKMVTAITEATVPKLCV
                     VVRKAYGAGLYAMCGPAFDPEATIALPTAKIAVMGPDAAVNAVFANKIAAIEDEDEQA
                     AFVAEQIEVYEADVDLLRLASELVIDAIVPPSALRDEVVRRFDAAGTRRREFSDRRHG
                     VPPV"
     gene            2156..3034
                     /locus_tag="VLR27_16170"
     CDS             2156..3034
                     /locus_tag="VLR27_16170"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HSP05049.1"
                     /translation="MSWDLPDTISIRDVGPRDGLQPEQPVRVADRVALITALVDAGVH
                     DVEAAAFVSPRAVPAMAGAADLFRALERDEHARYWALVPNVKGAELAVEAGVDGLTVT
                     VSASEAYSQRNVKMSVEESVAQIGAIAETAGSRPVDVVISCAFGSPYDEDIGAFDVAA
                     IVDSAQDAGATAITLADTTGVATPDRIADVVATTGIDVRLHLHDTRGTALVNAFAAMT
                     LGVAAFDTAVGGLGGSPFAEGAGGNLATEDLVHLCDGLGIDTGIDLDRMVRTAGTVSA
                     LIGRPVPSRITSTLGR"
     gene            3038..3241
                     /locus_tag="VLR27_16175"
     CDS             3038..3241
                     /locus_tag="VLR27_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05050.1"
                     /translation="MPYCDSCDRFYNPNTLDQEGDCPEGHHVADPTEPEPLPPVPWHF
                     KLMLALLVAYLLWRFVQFLAAVF"
BASE COUNT          460 a         1231 c         1122 g          464 t
ORIGIN      
        1 cttcgacgcc aaggtctcgc tcggcaccgg ctcagccagc agtcccaggt ccagctcgcc
       61 gagctcgatg cggcggacca ggtcgtcggt gcgctcctcg gtgaggacgg gctcggcccg
      121 ggggtgacgc cggcgcagct cccgcaccag cgtgggcagg aggtaggggg ccatggtggg
      181 gatgatgccg atccggaccg gcccgaccag ctcgccctgg aggtccaccg cggcatcggc
      241 gacgtcgtcc accgcccgga tggcccggcg ggcagcgtcg atgacggcca cggcgtggtc
      301 gggcacggac accccacccc gtccccgctc gaacagcgac agcccgaggc gctgctccac
      361 ctcgcgcacc tgggtcgaga gggccggctg gctgacaccg caggtctcgg cggcagcgct
      421 gaagctcagg tggtcggcga cggcgacgac gtactcgagc tgacgcaccg tcgggcgctg
      481 gcgggcaggg ggcatagcga gaggctatcg acactcgatc ccggatcacc tgcgggtcag
      541 cacctccgaa ctggcagtcg tggcgttgca cttcgcgccc actggccgga ccgggcaaga
      601 tcgtggggtg acggcgcccc acgacccgct cgagcacctc cgggctgcga cctcccgggc
      661 gctggccggc aacctcgaga cgcaggccgc caagctcgag aagcagggca agctcttcgt
      721 ccgcgaccgc ctcgacctgc tgctcgatgc cgggtccttc gtggaggacg cgctcctggc
      781 caacaaccag ggcgaggacc tgccggcgga cggtgtcgtc accggcacgg gcaccatcga
      841 cggccgtcgc gtgtgcgtgg tggccaacga cccgacggtg aaggccgggt cctggggggc
      901 tcgcacggtc gagaagatga tccgcaccag cgagtacgcc ctcgcccacg acctgcccat
      961 cttctggctg gtcgactccg ccggcgcccg catcaccgac caggtgcagc tgttccccgg
     1021 ccgccggggc gccgggcgga tcttcgccaa ccagatccgc ctctccggcc gggtgccgca
     1081 gatctgctgc ctgttcggac cctccgccgc cggcggcgcc tacatcccga gcttctgcga
     1141 cgtggtcttc atggtcgagg gcaacgcctc gatgtacctg ggctcgcccc gcatggccga
     1201 gaaggtcatc ggcgagcaca ccacgctcga ggagatgggt ggggcgcgga tgcacgccag
     1261 cgtctcgggc tgcggcgatg ccctggccca cgacgacgag gacgccctgg accaggcccg
     1321 ggcctggttc tcgtacttcc cgcagacctg gcgcgagcag ccgccccgct acgagccggt
     1381 gccgccggcc cacgacctcg ccgacgacgt cgtgccggtg gaggaggcgg tggccttcga
     1441 catgcacgcc ttcatcgacg ggctcgtcga tgccgagtcg ttcttcgagt acaagccgct
     1501 cttcgcccag gagctgatct gcgggctcgg tcgcatcgac gggcgcccgg tcggcgtcgt
     1561 ggccaacaac ccgtcccacc tcggcggcgt gctgttcgtg gactcggccg acaaggcgtc
     1621 ccgcttcgtg tggctgtgcg acgcgttcaa catcccgctg ctgttcctgg ccgacgtgcc
     1681 cgggttcatg atcggcaccc aggtcgagcg acaggggatc atccgccacg gcgccaagat
     1741 ggtcaccgcc atcaccgagg ccacggtgcc caagctctgc gtcgtcgtcc gcaaggccta
     1801 cggcgccggc ctgtacgcca tgtgcgggcc ggcgttcgat cccgaggcca ccatcgcgct
     1861 gcccaccgcc aagatcgccg tcatgggacc cgacgccgcc gtgaacgcgg tgttcgccaa
     1921 caagatcgct gccatcgagg acgaggacga gcaggcagca ttcgtcgccg agcagatcga
     1981 ggtgtacgag gccgacgtcg acctcctccg cctggcgtcc gagctggtga tcgacgccat
     2041 cgtccccccg agcgccctgc gcgacgaggt cgtgcgccgc ttcgacgcgg ccggcacccg
     2101 ccgccgtgag ttctccgacc gccgccacgg cgtgcccccg gtgtaggagc gagccatgtc
     2161 ctgggacctg cccgacacca tctccatccg cgacgtgggc ccccgcgacg ggctccagcc
     2221 cgagcagccg gtgcgggtgg ccgaccgggt cgcgctcatc accgcactcg tcgacgcagg
     2281 cgtccacgac gtggaggccg ctgccttcgt ctcaccccgc gccgtgccgg cgatggccgg
     2341 ggcggccgac ctgttccggg cgctcgaacg cgacgagcac gctcgctact gggcgctggt
     2401 cccgaacgtg aagggcgccg agctggccgt ggaggccggc gtcgacgggc tcaccgtcac
     2461 cgtgtcggcg tccgaggcct actcccagcg gaacgtgaag atgagcgtcg aggagtccgt
     2521 cgcccagatc ggcgccatcg ccgagaccgc cggcagccgg ccggtggacg tcgtgatctc
     2581 gtgcgcgttc ggctcgccct acgacgagga catcggcgcc ttcgacgtgg ccgccatcgt
     2641 ggactcggcc caggacgccg gtgccaccgc catcacgctc gccgacacca cgggcgtggc
     2701 gaccccggac cgcatcgcgg acgtggtggc caccaccggc atcgacgtcc gcctgcacct
     2761 gcacgacacg cgtggcaccg ccctggtgaa cgccttcgcc gccatgaccc tcggcgtggc
     2821 cgccttcgac accgccgtcg gcgggctcgg cgggtcaccg ttcgccgagg gggccggcgg
     2881 gaacctcgcc accgaggacc tcgtgcacct ctgcgacggg ctcgggatcg acaccggcat
     2941 cgacctcgat cgcatggtcc gcaccgccgg cacggtgtcc gcgctcatcg gccgcccggt
     3001 cccgtcgcgg atcacctcca cgctcgggcg gtagccggtg ccgtactgcg actcctgcga
     3061 tcgcttctac aacccgaaca ccctcgacca ggagggcgac tgccccgaag gccaccatgt
     3121 ggccgacccg accgaacccg agccgctccc gccggtcccg tggcacttca agctgatgct
     3181 ggcgctgctg gtcgcctacc tgctctggcg cttcgtgcag ttcctcgccg ccgtgttctg
     3241 agcatcgggc ggattggcgg atcgccacca gcagggg
//
LOCUS       DATISU010000410         4588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_407969, whole genome shotgun sequence.
ACCESSION   DATISU010000410 DATISU010000000
VERSION     DATISU010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407969"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VLR27_16180"
     CDS             <1..500
                     /locus_tag="VLR27_16180"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSP05051.1"
                     /translation="FDPDDDAPTDAEVLAAATGETPHDVCPEPGWYPVPMAPPMAAAA
                     LGLRCPTLTEVVAGITWPPGMDVGLVETVGGVRSPLAEDGDSRDLVRALDVDLVVLVA
                     DAGLGTIDAVRSGVDSLAPLSVVVLLNRFDEGDDLHRRNRDGLVQRDSFDVVTDVEDL
                     AARLR"
     gene            complement(620..838)
                     /locus_tag="VLR27_16185"
     CDS             complement(620..838)
                     /locus_tag="VLR27_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05052.1"
                     /translation="MATDETDAELDEIQDRVDRIRDRLPDNPGLDVVDKDDVPAYFDD
                     DDIPAEGVSKEEAVRRDDPDDHQAHAPG"
     gene            complement(925..3156)
                     /locus_tag="VLR27_16190"
     CDS             complement(925..3156)
                     /locus_tag="VLR27_16190"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HSP05053.1"
                     /translation="MSNATPKIIYTYTDEAPALATHSLLPVIETFASAAGVEVETRDI
                     SLAARILAVFPDALTDDQRVPDALGELGQLAKTPEANIIKLPNISASIPQMKAAIKEL
                     QDKGYALPDYPEDPQTDDERATRDRYDSVKGSAVNPVLREGNSDRRAPKAVKEFARKN
                     PHRMGEWSSDSKTHVATMSSGDFRSNEQSVTIDADTTLRIVHVSDSGEETVLKDGISV
                     LAGEVVDGTFMSAAQLRTFLAEQIADADAKDVLFSLHMKATMMKVSDPIIFGHAVEVY
                     FADVFAEHGDALRAAGANPRDGLGDVLAAVEQLDTDTKLAVQTGIAAALESGPDLAMV
                     DSDRGITNLHVPSDVIIDASMPPMIRDSGKMWNPAGELQDTKATIPDSSYAGVYQAVI
                     DDHKAHGALDPRTMGTVPNVGLMAQKAEEYGSHDKTFEVPAAGVVRVLDDAGTQLMEH
                     QVEAGDIWRMCTVKDAAIQDWVKLAVNRARATGSPAVFWLDGQRAHDEQLIRKVHRYL
                     EDHDSHGLDIEIMDPASATTYSLERIRRGEETISVTGNVLRDYLTDLFPILEVGTSAK
                     MLSIVPLMNGGGLFETGAGGSAPKHVQQFVKENHLRWDSLGEFLALAVSFEHLADHFD
                     KPRAQVLADTLDAATGALLQNGKGPSRKVGELDNRGSHYYLARYWAEALAEQTADADL
                     AERFAPLAQALAENEQTILDELNGAQGNPVDTGGYYFADRSVLDEAMRPSPTFNRIID
                     EAR"
     gene            3279..3920
                     /locus_tag="VLR27_16195"
     CDS             3279..3920
                     /locus_tag="VLR27_16195"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HSP05054.1"
                     /translation="MARQQGPQREGIALDDGTWVDVGRDGRWHPSAAERVRAAVILGV
                     FVGLLAVLALVLSVGDDGDGDEDLAAARDTTTTTEPPELTTTTTLPPDPSSVGGEPAP
                     IHCVGDDRGGAELRERDTTAVLVLNATSRTGQAADIVERLEGLGYATTVPGNAGRLAV
                     SNFAYVSGYCAEAVRAAFELGLTEATFEPVDAESLSVPLGRARIVATVGADSL"
     gene            complement(3940..>4588)
                     /locus_tag="VLR27_16200"
     CDS             complement(3940..>4588)
                     /locus_tag="VLR27_16200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HSP05055.1"
                     /translation="GKTTILRVIMTLERPDAGTIQVGGRHLYHEVHGNGLKPASEKHI
                     RSVRSDIGMVFQHFNLFPHMTALENIMLAPVKVHGKSRDEARARGMELLDSVGLAHAA
                     NQTPGQLSGGQKQRVAIARSLANEPRIMLFDEVTSALDPELVGDVLEVLRRIAEEGKT
                     TMMLVTHEMGFAREVADRVVMFDAGAVVEEGPPAQILDNPREERTRSFLGAVREH"
BASE COUNT          583 a         1506 c         1746 g          753 t
ORIGIN      
        1 cgttcgaccc ggacgacgac gcacccaccg acgccgaggt gctggcggcg gccacgggcg
       61 agactccgca cgacgtgtgc ccggagccgg gttggtaccc cgtaccgatg gcaccgccga
      121 tggcggccgc ggcgctgggc ctgcggtgcc cgacgctcac cgaggtggtc gccggcatca
      181 cctggccacc cgggatggac gtcgggttgg tcgagacggt gggcggtgtc cgctcgccgc
      241 tggccgagga cggcgacagc agggacctgg tccgtgcgct cgacgtcgac ctcgtggtgc
      301 tggtcgccga cgccggcctc gggaccatcg acgcggtgcg atcgggcgtc gactcgctcg
      361 cgccgctgtc cgtcgtggtc ctgctgaacc gcttcgacga gggcgacgac ctgcaccgcc
      421 gcaaccgcga cgggctggtg cagcgcgaca gcttcgatgt ggtgaccgac gtcgaggacc
      481 tcgccgcccg actccgctga ccccggtccg ttctggccta catcgagcac caccagacgg
      541 cacggatgta ggccagaacg aggttcagtc ggggccaggg cgcgcgacac cagcactgcc
      601 agtttccaga gcggggggac taccccgggg cgtgggcctg gtggtcgtcg gggtcgtccc
      661 ggcggacggc ctcttccttg ctgacgccct cggcggggat gtcgtcgtcg tcgaagtagg
      721 cggggacgtc gtccttgtcg accacgtcga ggcccgggtt gtcgggcagt cggtcgcgga
      781 tgcggtcgac ccgatcctgg atctcgtcca gctcggcgtc ggtctcgtcg gtggccatgg
      841 gtgcctcctg aggtggtggg gcgtgctgcc ccggtccctt cccccgggac cgggacgcca
      901 tgcccgagcg gaggcggccg gccctcagcg ggcttcgtcg atgatccggt tgaaggtcgg
      961 gctgggccgc atcgcctcgt cgaggacgga ccggtcggcg aagtagtagc cgccggtgtc
     1021 gaccgggttg ccctgagcgc cgttgagctc gtcgaggatg gtctgctcgt tctcggccag
     1081 ggcctgggcg agcggggcga agcgctcggc caggtcggcg tccgcggtct gctcggccag
     1141 ggcctcggcc cagtagcggg ccaggtagta gtggctgccg cggttgtcga gctcgccgac
     1201 cttgcgggac gggcccttgc cgttctgcag cagcgcaccg gtggcggcgt cgagggtgtc
     1261 ggccagcacc tgggctcggg gcttgtcgaa gtggtcggcc aggtgctcga aggacacggc
     1321 cagggccagg aactcgccga gggagtccca gcggaggtgg ttctccttca cgaactgctg
     1381 gacgtgcttg ggggcggagc cgccggcgcc ggtctcgaac agcccgccgc cgttcatcag
     1441 cggcacgatc gacagcatct tggcgctggt gccgacctcg aggatcggga acaggtcggt
     1501 gaggtagtcg cgcagcacgt tgccggtgac cgagatggtc tcctcgcccc gtcggatgcg
     1561 ctcgagggag taggtggtgg ccgaggccgg gtccatgatc tcgatgtcga ggccgtggga
     1621 gtcgtggtcc tcgaggtagc ggtggacctt gcggatcagc tgctcgtcgt gggcgcgctg
     1681 cccgtcgagc cagaacacgg ccggggagcc ggtggcccgg gcccggttga cggccagctt
     1741 gacccagtcc tggatggcgg cgtccttcac cgtgcacatg cgccagatgt cgccggcctc
     1801 gacctggtgc tccatcagct gggtgccggc atcgtcgagc acgcgcacca cgccggcggc
     1861 ggggacctcg aaggtcttgt cgtggctgcc gtactcctcg gccttctggg ccatgaggcc
     1921 gacgttgggg accgtgccca tggtgcgggg gtcgagggcg ccgtgggcct tgtggtcgtc
     1981 gatgacggct tggtagacgc cggcgtagga cgagtccggg atggtggcct tggtgtcctg
     2041 gagctcgccg gccgggttcc acatcttccc ggagtcccgg atcatcggcg gcatggaggc
     2101 gtcgatgatg acgtcggagg gcacgtgcag gttcgtgatg ccgcgatcgg agtcgaccat
     2161 ggccaggtcg gggccggact cgagcgcggc ggcgatgccg gtctgcaccg cgagcttggt
     2221 gtcggtgtcg agctgctcga cggcggcgag cacgtcgccg aggccgtcgc gggggttggc
     2281 gccggcggcg cggagggcgt cgccgtgctc ggcgaacacg tcggcgaagt acacctcgac
     2341 ggcgtggccg aagatgatcg ggtcggagac cttcatcatc gtggccttca tgtgcaggga
     2401 gaacaggacg tccttcgcgt cggcgtcggc gatctgctcg gccaggaagg tgcgcagctg
     2461 ggcggcgctc atgaaggttc cgtcgacgac ctcgccggcc agcaccgaga tgccgtcctt
     2521 gagcacggtc tcctcaccgg agtccgacac gtgcacgatc cgcagggtgg tgtcggcgtc
     2581 gatggtgacc gactgctcgt tgctgcggaa gtcaccgctg ctcatggtgg cgacgtgggt
     2641 cttggagtcc gaggaccact cacccatgcg gtgcgggttc ttgcgggcga actccttgac
     2701 ggccttgggg gcgcgccggt cggagttgcc ctcgcgcagc accgggttca ccgcgctgcc
     2761 cttgaccgag tcgtagcggt cgcgggtggc gcgctcgtcg tcggtctggg gatcctctgg
     2821 gtagtcgggg agcgcgtagc ccttgtcctg cagctccttg atcgccgcct tcatctgcgg
     2881 gatcgacgcc gagatgttgg gcagcttgat gatgttggcc tcgggcgtct tggccagctg
     2941 gccgagctca ccgagcgcgt cggggacgcg ctggtcgtcg gtgagcgcat cggggaacac
     3001 ggcgaggatg cgggcggcga gggagatgtc gcgggtctcg acctccaccc cggcggcgct
     3061 ggcgaaggtc tcgatgaccg ggagcagcga gtgcgtggcc agcgccggcg cctcgtcggt
     3121 gtaggtgtag atgatcttcg gggtcgcgtt gctcatgggc tcttccgtcg tcggtcgtct
     3181 gggcgcagcc ggggggccgc cacgtcttcg gaggctaccc acctcccgcg ggggcgatgc
     3241 caccacgcag tggctccgga gtggcagtgt cgggaccggt ggcacgccag cagggaccac
     3301 agcgcgaagg gatagcgctc gacgacggga cgtgggtcga cgtgggccgc gacgggcggt
     3361 ggcacccgtc cgcagcggag cgcgtacggg cggcggtcat cctcggggtc ttcgtggggc
     3421 tgctcgccgt gctcgcgctc gtgctgtccg tcggcgatga cggcgacggg gacgaagacc
     3481 tggccgccgc cagggacacg acgaccacca ccgaaccgcc cgagctgacc accaccacca
     3541 cgctgccgcc cgatccgtcg tcggtgggcg gggagccggc tccgatccac tgcgtcggcg
     3601 acgaccgggg cggggccgag ctgcgtgagc gggacaccac cgcggtgctg gtcctgaacg
     3661 ccaccagccg caccggccag gccgccgaca tcgtcgagcg cctcgagggc ctgggctacg
     3721 ccaccacggt gcccgggaac gccggccgac tggcggtctc caacttcgcc tacgtctcgg
     3781 ggtactgcgc cgaggccgtg cgcgccgcgt tcgagctcgg gctcaccgag gcgacgttcg
     3841 agccggtgga cgccgagtcc ctctccgtgc cactgggccg ggcccggatc gtggcaaccg
     3901 tcggagccga ctccctgtag cgcctatcgg ctcggctcct cagtgctcgc gcaccgcgcc
     3961 gaggaacgag cgggtccgct cctccctcgg gttgtcgagg atctgggcgg gagggccttc
     4021 ctcgaccacg gcaccggcgt cgaacatcac gacgcggtcg gcgacctcgc gagcgaagcc
     4081 catctcgtgg gtgaccagca tcatggtggt cttgccctcc tcggcgatcc ggcgcagcac
     4141 ctcgagcacg tcgccgacga gctcggggtc gagcgccgag gtcacctcgt cgaacagcat
     4201 gatccggggt tcgttggcca gcgacctggc gatggccacc cgctgcttct ggccgccgga
     4261 gagctgaccc ggtgtctggt tcgcggcgtg ggcgaggccg acgctgtcga gcagctccat
     4321 gccccgggcc cgggcctcgt cgcgggactt cccgtgcacc ttcaccggcg ccagcatgat
     4381 gttctccagc gcggtcatgt gcgggaacag gttgaagtgc tggaacacca tgccgatgtc
     4441 ggagcggacc gagcggatgt gcttctcgct ggccggcttg agcccgttgc cgtggacctc
     4501 gtggtagagg tgccgcccac cgacctggat cgtgccggcg tcggggcgct cgagggtcat
     4561 gatcacgcgc aggatcgtgg tcttgccc
//
LOCUS       DATISU010000411        16487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_594382, whole genome shotgun sequence.
ACCESSION   DATISU010000411 DATISU010000000
VERSION     DATISU010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 16487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16487
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594382"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VLR27_16205"
     CDS             complement(<1..650)
                     /locus_tag="VLR27_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HSP05056.1"
                     /translation="MGPLQGTTVLEIAGIGPGPFCAMLLADMGADVIRVDRAGQVMGG
                     DPESPPADVLNRGRRSIGVDLKSSEGVETVLALVEQADVIMEGFRPGVAERLGIGPEE
                     CRARNPKIVYGRMTGWGQDGPYAQAAGHDINYIALAGALEPIGRRGEAPVPPLNLVGD
                     FGGGGMFLAFGIVCALLEAQRSGEGQVVDAAMVDGAAVLTTMFHAFRAMGIWNDERG"
     gene            complement(684..1595)
                     /locus_tag="VLR27_16210"
     CDS             complement(684..1595)
                     /locus_tag="VLR27_16210"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP05057.1"
                     /translation="MGETIHQVNGIELCAEVRGPTDGVPLLLVMGLGAQLVSWEDDFV
                     SRLVDRGYRVARFDNRDVGRSTHFDDADIDVMATLRALAKGEAAGVPYTLSDMAADAA
                     ALLDELGMSPAHVVGASMGGMIVQAMAIEQPQAVRSVVSIMSTTGDRDVGQPTAEANE
                     LLFSPPPTTEDEAVERARASEATWGSPAHRDPDAAVERARREWNRVRNPAGVGRQIVA
                     INASGSRTDALGGVQVPFTVIHGDADTLVTPTGGRRTAEAVPHAVHVELEGMGHNLPR
                     ALWPRIMDEIDATVERTRDADPTRHTD"
     gene            1657..2661
                     /gene="galK"
                     /locus_tag="VLR27_16215"
     CDS             1657..2661
                     /gene="galK"
                     /locus_tag="VLR27_16215"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HSP05058.1"
                     /translation="MSKQTDVRAFAPGRVNLIGEHTDHTGGRCLPMAVAMGVTVHGRR
                     EGDRVLLSSAEVPGVADIALDVEDPATVEPAWARYVAGVVAELRPEVGFTGGLRSDVP
                     VGAGMSSSAALEVAVALVLGHTGDVDDLARRCQAAEQRATGVPCGILDQLASAAARDG
                     HAFVLDCHTLERTHVPVPDTIEVRVVHSGQERTLVGSAYAERRAECEAAEAAIGPLRL
                     ASPADAATIDDPVLRRRARHVTTENQRVLDAADALRAGDVVALGALLDEGHRSLRDDF
                     EVSTPTVDATVDRLRARPGVHGVRLMGGGFGGCVVAVTDPGALDEGWHARPGPGAHLM
                     "
     gene            complement(2718..3344)
                     /locus_tag="VLR27_16220"
     CDS             complement(2718..3344)
                     /locus_tag="VLR27_16220"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HSP05059.1"
                     /translation="MPFPRKLLNEAEEVVLDLHPHWSFLAKQTLALLAAIVLGALVLL
                     NVDNGIVQLLAGVLILAALGWWIVRYLDWRTTNFVVTSDRLIYRHGVVAKHGIEIPLE
                     RVNNVLFSQRLIERMLGSGDLVIESAGESGRQAFSDVRKPSAVQNEIYRQIEANENRK
                     FDRIGQGHGGGGSSTSVADELAKLDELRRNGILSEDEFAAQKARLLGR"
     gene            complement(3376..3726)
                     /locus_tag="VLR27_16225"
     CDS             complement(3376..3726)
                     /locus_tag="VLR27_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HSP05060.1"
                     /translation="MVDVDPERFEELVAAALDELPGPLAEGLENVMVRIRTGRPTGSL
                     LGLYEGVPLTERGDYGAGGPPMPDRVTIYRLPICSMCETEDEVTEQVRITVIHELAHH
                     YGIDDDRLHELGWA"
     gene            3770..5701
                     /locus_tag="VLR27_16230"
     CDS             3770..5701
                     /locus_tag="VLR27_16230"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSP05061.1"
                     /translation="MDPEAVLAGLDDDQRRAATSPASPLAVVAPAGSGKTRVLTARVA
                     HRIAAGEIEPAHVLCVTFTRKAAGELTSRLRRIGVRDRIEAGTFHAVAWRQLRDRWSA
                     QGRAEPQLLDRVRPFLREQLEVTAPAALTELANEIGWARARMLRPDDYADAARAAGRR
                     TRHPAAEVAERFAQYEAAKAKRHVVDFDDLLAACAAAIEGDPGFAAAQRWRFRHLFVD
                     EFQDVNPLQFRLLEAWRGDRWDVFVVGDTHQSIYGWNGADPGLLDELGRRWPALETIH
                     LDRTHRSTPQITAAAASVIAAAGLPDRHPVSSGAPGPPARCLEHEDEVAEIRAIGRAI
                     RRRRGSDLPWKSFAVLARTHERVLDLDRGLAALGVPTRMRRQASLLDDPAVRRVLHRL
                     RDDERPVLTALGDALRGDAEDPAVVVLAEAAEGQRTKDDAMTGRAFVGWAYANLSDDD
                     HEPDAVTISTIHAAKGLEWPVVHVAGCEDGSIPISSARRREARAEEARLLYVAVTRAE
                     REVTLHWARARTHRGTRRSQTRSPFLAGFEERVAAVTDPPTSTGVPQLAALRAQLHPP
                     EVTDPLLAALRAWRTARARAARTEPGTVVPDEVLVEIAAKRPRTRPEIDAVAGLGPAR
                     RNRFADELLAVVARAEHAR"
     gene            5698..6192
                     /locus_tag="VLR27_16235"
     CDS             5698..6192
                     /locus_tag="VLR27_16235"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HSP05062.1"
                     /translation="MRFTVEQRFSTGADETARAFADPDLYAGFAALPKVSVPDVLSCE
                     RNGDLVELRIRYRFAGHLSSAARAVIDPAQLTWVDESTHDLARRLVRFVLRPDHHGNR
                     FRCSGDYRIEPHGEGCRRHATIDLKVSAPFVGGAVERAIESGLREHLADETEVVEAYL
                     AARR"
     gene            6202..6993
                     /locus_tag="VLR27_16240"
     CDS             6202..6993
                     /locus_tag="VLR27_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HSP05063.1"
                     /translation="MRLATWNVNSLKARLERVEAWLAEASPDVLCVQETKLADDAFPA
                     LALGALGYDGVHHGQGRWNGVAILSRIGISDVTHGFADGGPPDDEARLLTARCGTTVV
                     VTAYAPNGRAVGHEQFHYKLAWFDRLAAHLDAVADPSDDVVVCGDLNIAPEDRDVWDV
                     DAIHGATHITEDERHRFQRLLDWGLVDAFRRCYPDDDRLFTWWDYRAGNFHKHKGMRI
                     DHVLVSEPLASRLSWSLVDRNARKGTGPSDHAPLLIEVASEAVPA"
     gene            6990..7742
                     /locus_tag="VLR27_16245"
     CDS             6990..7742
                     /locus_tag="VLR27_16245"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HSP05064.1"
                     /translation="MNDADADAGGQAFGPNSPSWPSRLAELASEEPTPRRRELRRLAD
                     ATRRVLHTMVMNGADADTLAAAADALEAVADDLHHHAQPSQSIYEGFGEAATSGNPHG
                     FFDHSPMLGVANPIAPPIVLRGIDERTMEGAATFGPAYEGPPGCVHGGYIAAAFDEVL
                     GAVQSLSGQPGMTGTLIVRYRSPTPLHEPLRFTGRFEGVEGRKILTTGTLHAGDRLCA
                     EAEGIFISVDFGRMAELRSRRAEQQARRDDRT"
     gene            complement(7744..9438)
                     /locus_tag="VLR27_16250"
     CDS             complement(7744..9438)
                     /locus_tag="VLR27_16250"
                     /inference="COORDINATES: protein motif:HMM:NF023403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HSP05065.1"
                     /translation="MAAVTPVGSRRSGSGIAAAGPAPPNLLRHTLPTTHLVVDGSNIA
                     TEGRSLPSLQQLLEAVDAVTAEFDHANVTVIVDATFGHRIDASERDQYEEKVNSGQII
                     TPPAGVIGRGDAFILEVADRADAIILSNDSFQEFHGTYDWLFQEGRLLGGKHVPGVGW
                     IFLARSPVRGPTSRRAVSDAKRKRRGSKTAASKTPASSRPSKSSSKSTKSSRSKSSSK
                     SAKSSGRSKAKAKTDEPAGRKRGGKEPEPYNEPLPFIEFVGAHPVGSVIEAEIEQFAS
                     HGAYVLCDGTRAYISLKSISDPPPRSAREVFELGETREFVVKAIDTPRRGIDLAMPGM
                     ALDAPDDDEIDTGDDAIEVEDAPTAEAAPAAAPVIDDGDRKLSVSQRRKLRAQQQAEA
                     EAAAAAAAAEADAPAKKAASRTRARAAAKKAAPDQKTTDRKATSPMATKKAASKKKAA
                     AKKAATKKKAAPAKKATAKKSAPASRAKKSPAKKATTKKAAAKKSSAKKATTKKAAAK
                     KSPAKTSSAKKATTKKAAAKKSPAKKATTKKAAAKKSPAKKSTTKKAASKRATKRS"
     gene            9482..10138
                     /locus_tag="VLR27_16255"
     CDS             9482..10138
                     /locus_tag="VLR27_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HSP05066.1"
                     /translation="MPDPDAQITAALFDFGGVILSSPFDAFADYEREHGLPEGFIRGL
                     NATNPDDNAWAKMERNDVTIPEFCELFEAEARAAGGELDAAAVLARLRGQIRPAMVEA
                     VRRCKARLKTACLTNNFVAPDATHPNGRMDPESFDGVMDLFDVIVESSKAGCRKPDPR
                     FYEIACEELGIEPSEAVFLDDLGINLKPAKAMGMTTIKVVDPDEAIAELERVVGFPLR
                     "
     gene            10193..11008
                     /locus_tag="VLR27_16260"
     CDS             10193..11008
                     /locus_tag="VLR27_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP05067.1"
                     /translation="MSVRARHNVQISGVPDGQPLVFAHGFGCDQHMWRHVAPRFEDRY
                     QVVLFDHVGAGGSQTEAYDPERHASLDGYAEDVVTLVEGLDLHGVVFVGHSVSAMIGI
                     LAARRAPERFAHLVLVGPSPRYIDDPDSGYVGGFSAPDIDELLASLESNYLGWSSAMA
                     PVIVGNPERPELGVELTESFCKADPEIQQRFAKATFLSDNRADLAHVPVPCLVLQCRD
                     DVIAPESVGRYVDDQLPESELVILDATGHCPNLSAPDETTAAIEAYLRRHRDR"
     gene            11005..12210
                     /locus_tag="VLR27_16265"
     CDS             11005..12210
                     /locus_tag="VLR27_16265"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSP05068.1"
                     /translation="MSGLSAVGASGPTHGYVQALIEDDPAELYETAPCGYLSTDPSGA
                     IVKVNRTFLDWTGYGRDELVGSRRFQSLLAVGDRIFYETHFAPSLAMQGTVREIAVEV
                     VCADGERLPALVNAVSTDAVGGRPGRIRIAVFDARERRSYERELLIARERAEQAAAEA
                     RALAETLQRTLLPLSPPEIPGMDVGTAYRPAGDGSVVGGDFYDLFELRDGTWGVTLGD
                     VCGKGAPAAMVTALARHTVRALAVRSAKPSEVLRGLHDAIARSDVDSFCTALYLTVCA
                     EDGALEVCMSAGGHHLPLRVGVDGTVAEVGRPGHLLGMLEPLHLHDEDVAVGPGEKLV
                     LYTDGVIEAARAGDFFGEGRLHDVIREHRGDAAQDLADAIAGTAVEFQEQDTRDDIAV
                     VVLGAAAST"
     gene            complement(12200..15829)
                     /locus_tag="VLR27_16270"
     CDS             complement(12200..15829)
                     /locus_tag="VLR27_16270"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HSP05069.1"
                     /translation="MAEKQTPDLVGPNAWMVDEMYEQYLSDPSSVSESWQDFFADYKR
                     DSDAEPIAASPAATNGAGAPAKPAPAPAPEPEAKKSDEKKAEAAEDEVGEPIRGVGAR
                     IVENMERSLAVPTATSYRNVPAKLLEVNRAVINGYLGRTRGGKVSFTHLIGYAVVRAI
                     ADTVPAMNNSYVEGADGKPRIVRNDHVGLGIAVDIEKADGSRSLLVPVVKDADTLDFR
                     GFWGSYEELIRKVRNNKLAPDDFSGITVSLTNPGTIGTVQSVPRLMPGQGLIVGVGSI
                     DWPAEMQAADPETLADLGLSKVITISSTYDHRIIQGAESGVFLKRVHELLLGEDGFYD
                     EIFRALDVPYEAVQWRQDVRPVDQEAYRREKQMAVQRLINMYRVRGHLMADLDPLRWR
                     EPNMHTELDPLTYGLTIWDLEREYLTGGLAGREKLTLRQILKVLRDAYCRTLGIEYMH
                     IQDPEQKAWIQEQVEGVEVALDPDEQHHVLGRLNAAEAFEKFLATKYVGQKRFGLEGG
                     ESAIVILDAVLERAAAEGMDKAIMGMAHRGRLNVLVNIAGKDLDQLFSEFEGYVPEGS
                     VQGSGDVKYHLGQEGVYCSRHEQEITVELAANPSHLEAVDPVVVGMVRALQDQIDQPE
                     AFSVLPILIHGDAAFAGQGVVVETLQMSDIRGYRVGGTVHLIVNNQIGYTTTPEASRS
                     GFYSTDVAKMIQAPVFHVNGDDPEACVRAARLAFEFRQRFNKDVVIDMVCYRRHGHNE
                     GDDPSYTQPMMYAKIDQHRSVRKLYTESLVKRGHMSLEEAEAALDDFQARLQDALDET
                     RSSPEEHMAAEPPAARGVLPHIETGVDREVLDRVFAALDSTPEDFNLHPKLAKQFDTR
                     RKMWESGEVDWGLAESLAFGTLLLEGIDIRLAGQDTRRGTFAHRHATLHDHTNGAEYV
                     PLGNIADDQGKLWIYDSLLSEYAALGFEYGYSVENPKAMVAWEAQFGDFVNGAQIIID
                     QFIVAAADKWQQDSGLIMLLPHGFEGQGPEHSSARMERFLLLCAEDNIQVVNATTAGQ
                     FFHLLRRQMHQEARRPLIVFTPKSLLRAKPARSSVEELTHGSFEEVLPDPAVASGAID
                     TDDVRRVVLATGKVAQDAIASRDERQVPVAIVRVEQVYPWPGARLAEELERYPKATEL
                     IWLQEEPENMGAWNFASRRLYEGFGDSHKIQRVSRYESGSPATGANKIHHQEQAMILD
                     KALTY"
     gene            complement(15882..>16487)
                     /locus_tag="VLR27_16275"
     CDS             complement(15882..>16487)
                     /locus_tag="VLR27_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05070.1"
                     /translation="LLAGRLDEAEVDLRDGGRLHRAVGGAVGEALALQRLAEVAIHRG
                     RDREAWDLLDEALDLARVTDIGFHLLDRIYGTRITLAGRDPDAALAAVDEAESAVQGQ
                     LETCPGCRITFAVPAAIACATAGELDRALRYEEASAWLAQVVMRLPAWDAAHEEVRGH
                     VRLAAGDAGLAKQHFARAASGFGAAGHPLDAERCRSLVAAG"
BASE COUNT         2166 a         5961 c         5904 g         2456 t
ORIGIN      
        1 ccccgctcgt cgttccagat gcccatggcc cggaaggcgt ggaacatcgt ggtgagcacg
       61 gcggcaccgt cgaccatggc ggcgtcgacg acctggccct caccggagcg ctgggcctcg
      121 aggagcgcgc agacgatgcc gaacgcgagg aacatgccgc cgccgccgaa gtcgcccacc
      181 aggttgaggg gcggcaccgg ggcctcgccg cggcgaccga tgggctcgag cgcgccagcg
      241 agggcgatgt agttgatgtc gtggccggcg gcctgggcgt agggcccgtc ctggccccag
      301 ccggtcatgc gcccgtacac gatcttgggg ttgcgggccc tgcactcctc gggcccgatg
      361 ccgaggcgct cggccacgcc gggacggaag ccctccatga tcacgtcggc ctgctcgacc
      421 agggcgagga cggtttcgac gccctcggag ctcttcaggt ccaccccgat ggagcggcgg
      481 ccgcggttca gcacgtcagc aggcggtgac tcggggtcgc cgcccatgac ctgcccggcg
      541 cggtcgacgc ggatcacgtc ggcgcccatg tcggcgagga gcatggcgca gaacgggccg
      601 ggcccgatgc cggcgatctc gagaacggtg gtgccctgga gcggtcccat gtggcggggt
      661 tcctctttcg tggtgggggt ggttcagtcg gtgtggcggg tggggtcggc gtcgcgcgtc
      721 cgctcgacgg tggcgtcgat ctcgtccatg atccgcggcc acagggcgcg gggcaggttg
      781 tgccccatcc cctcgagctc gacgtggacg gcgtggggga cggcctcggc ggtgcggcgc
      841 ccgccggtgg gggtgacgag ggtgtcggca tcaccgtgga tcacggtgaa gggcacctgc
      901 acgccaccga gcgcatcggt gcggctgccc gaggcgttga tggcgacgat ctgacggccg
      961 acgccggcgg ggttgcggac ccggttccac tcgcggcgag cgcgctcgac ggcggcgtcc
     1021 gggtcgcggt gagccgggct gccccacgtg gcctccgatg cgcgggcgcg ctcgacggcc
     1081 tcgtcctcgg tggtgggcgg cggcgagaag agcagctcgt tggcctccgc ggtcggctgg
     1141 ccgacgtcgc ggtcgccggt ggtcgacatg atcgacacca ccgagcgcac cgcctggggc
     1201 tgctcgatgg ccatcgcctg cacgatcatg ccgcccatcg atgcgccgac cacgtgggcc
     1261 ggcgacatgc cgagctcgtc gaggagcgca gcggcgtcgg ccgccatgtc cgagagcgtg
     1321 taggggacgc cggcggcctc acccttggcc aacgcccgca gggtggccat cacgtcgatg
     1381 tcggcatcgt cgaagtgggt cgaccgaccg acgtcgcggt tgtcgaagcg ggcgacgcgg
     1441 tagccccggt cgaccagccg gctgacgaag tcgtcctccc agctgaccag ctgcgcgccg
     1501 agccccatga cgaggagcag cgggacgccg tcggtgggtc cgcgcacctc cgcgcagagc
     1561 tcgatgccgt tgacctgatg gatcgtctcc cccatgccgg gatgcttgct gttccttgac
     1621 tgctcggtca agacccggcc cggtacggtc gccgccgtgt cgaagcagac cgacgtgcga
     1681 gccttcgccc cgggtcgggt gaacctgatc ggcgagcaca ccgaccacac cggggggcgc
     1741 tgcctgccca tggcggtggc gatgggcgtc accgtgcacg gccggcgcga gggcgaccgg
     1801 gtgctgctct cgtcggccga ggtgcccggc gtcgccgaca tcgcgctcga cgtcgaggac
     1861 ccggccacag tcgagccggc gtgggcccgg tacgtcgccg gcgtggtcgc cgagctgcga
     1921 cccgaggtcg ggttcaccgg cggcctccgc tccgacgtgc cggtcggcgc cgggatgtcg
     1981 tcgtcggccg cgctcgaggt cgcggtcgcg ctggtgctcg ggcacaccgg tgacgtcgac
     2041 gacctggccc ggcgctgcca ggcagccgag cagcgcgcca ccggagtgcc gtgcgggatc
     2101 ctcgaccagc tcgcctccgc cgccgcgcgg gacggccacg ccttcgtcct cgactgccac
     2161 accctcgagc gcacccacgt gccggtgccc gacacgatcg aggtgcgcgt ggtccactcc
     2221 ggccaggagc gcacgctcgt cggttcggcc tacgccgagc gacgggccga gtgcgaggca
     2281 gccgaggccg ccatcggccc cctccgactc gcctcccccg ccgacgccgc cacgatcgac
     2341 gacccggtgc tgcgccggcg ggcccgccac gtcacgaccg agaaccagcg cgtcctcgac
     2401 gcggccgatg cgctgcgggc cggcgacgtc gtcgccctcg gcgccctcct cgacgagggg
     2461 caccgctcgc tgcgggacga cttcgaggtc tccacgccga ccgtcgacgc caccgtcgat
     2521 cggttgcgag cccgaccggg cgtgcacggc gtgcgcctca tgggcggcgg cttcggcggc
     2581 tgcgtcgtcg cggtcaccga tcccggtgcg ctcgacgagg gctggcacgc ccgacccggc
     2641 cccggcgccc acctgatgtg acgttctggc ctacatcgat gaccaccagg tggcacatcg
     2701 gtaggccaga acggagctca gcggccgagg aggcgggcct tctgagcggc gaactcgtcc
     2761 tcggagagga tgccgttgcg gcgcagctcg tcgagcttgg ccagctcgtc ggccaccgag
     2821 gtcgagctgc cgcccccgcc gtggccctgg ccgatgcggt cgaacttgcg gttctcgttg
     2881 gcctcgatct ggcggtagat ctcgttctgc acggcggagg gcttccgcac gtcggagaag
     2941 gcctggcggc cgctctcgcc ggcggactcg atgacgaggt cgcccgagcc gagcatccgc
     3001 tcgatgaggc gctgggagaa caggacgttg ttgacccgct ccagggggat ctcgatgccg
     3061 tgcttggcca ccacgccgtg gcggtagatg agccggtcgg aggtgacgac gaagttggtg
     3121 gtgcgccagt ccaggtagcg cacgatccac cagcccaggg cggcgaggat caggaccccg
     3181 gccagcagct ggacgatgcc gttgtcgacg ttgagcagca cgagcgcgcc gagcacgatg
     3241 gcggcgagga gcgcgagggt ctgcttggcg aggaacgacc agtgcgggtg caggtcgagg
     3301 acgacctcct cggcctcgtt cagcagcttc cgagggaacg gcacgaccgg atggtaaccc
     3361 cggcgggcac ggcagctagg cccatcccag ctcgtggagg cgatcgtcgt cgatgccgta
     3421 gtggtgggcc agctcgtgga tgaccgtgat ccggacctgc tcggtcacct cgtcctcggt
     3481 ctcgcacatc gagcagatcg gcaggcggta gatcgtgacc cggtccggca tgggtgggcc
     3541 accggcgccg tagtcgcccc gctcggtgag cgggaccccc tcgtacaggc cgaggagcga
     3601 gccggtgggg cgaccggtgc ggatgcggac catgacgttc tcgaggccct cggccagcgg
     3661 gccgggcagc tcgtcgagcg ccgccgccac cagctcctcg aatcgctcgg ggtcgacgtc
     3721 caccatcgac gccgacgcta ccggggcagc ctctagcctc caggtcgtga tggaccccga
     3781 ggcggtgctg gccggcctgg acgacgacca gcgccgcgcc gccacctcgc ccgcgtcgcc
     3841 gctggccgtg gtcgcgcccg cagggtccgg caagacgcgg gtcctcaccg cgcgcgtcgc
     3901 ccaccgcatc gcggccggcg agatcgagcc ggcccacgtc ctgtgcgtca cgttcacccg
     3961 caaggcggcc ggcgagctca cctcccggct gcggcgcatc ggcgtgcggg accgcatcga
     4021 ggccggcacg ttccacgccg tggcgtggcg ccagctgcgg gaccgctggt cggcgcaggg
     4081 ccgcgccgaa ccccagctgc tcgaccgcgt ccgccccttc ctgcgcgagc agctcgaggt
     4141 gaccgcgccg gctgccctca ccgagctggc caacgagatc gggtgggccc gcgcccgcat
     4201 gctccgaccc gacgactacg ccgacgcagc ccgggccgcg ggtcggcgca cccggcaccc
     4261 ggccgccgag gtcgccgagc gcttcgccca gtacgaggcg gccaaggcca agcgccacgt
     4321 cgtggacttc gacgacctgc tcgccgcctg cgccgctgcc atcgaggggg acccgggctt
     4381 cgccgccgcc cagcgctggc gcttccgcca cctgttcgtc gacgagttcc aggacgtgaa
     4441 cccgctgcag ttccggctgc tcgaggcgtg gcggggcgat cgctgggacg tgttcgtcgt
     4501 cggcgacacg caccagtcga tctacggctg gaacggcgcc gaccccggcc tgctcgacga
     4561 gctcggtcgc cggtggccgg cgctcgagac catccacctc gaccgcacgc accgctcgac
     4621 gccgcagatc accgcggcgg cggcctcagt catcgccgcc gccgggctcc ccgaccgcca
     4681 cccggtctcc agcggcgccc cgggtccgcc ggcgcgctgc ctggagcacg aggacgaggt
     4741 ggccgagatc cgcgccatcg gccgggccat ccgccggcgc cgggggtcgg acctgccgtg
     4801 gaagtccttc gccgtcctgg cccgcaccca cgagcgcgtc ctcgatctcg accgcggcct
     4861 cgccgccctc ggcgtgccga cccgcatgcg ccgccaggcg tcgctgctcg acgaccccgc
     4921 cgtgcggcgc gtgctgcacc gcctccgcga cgacgagcgc ccggtgctca ccgcgctggg
     4981 cgacgccctc cgcggcgacg ccgaggaccc ggccgtggtg gtgctggccg aggcagccga
     5041 gggccagcgc accaaggacg acgccatgac cggacgcgcc ttcgtcggct gggcctacgc
     5101 caacctctcc gacgacgacc acgagcccga tgccgtcacc atctccacga tccacgcggc
     5161 gaagggcctg gagtggcccg tggtccacgt cgccgggtgc gaggacggat cgatcccgat
     5221 ctcctcggcg cgccggcgtg aggcccgggc ggaggaggcc cgactgctgt acgtcgccgt
     5281 cacccgcgcc gagcgcgagg tgaccctgca ctgggctcgg gcgcgcaccc atcgggggac
     5341 ccgacgatcg cagacccgga gcccgttcct cgccggcttc gaggagcgcg tcgccgcggt
     5401 gaccgatccg cccacctcga ccggcgtccc ccagctcgcc gcgctccggg cccagctcca
     5461 cccgcccgag gtcaccgacc cgctcctggc tgcgctgcgg gcgtggcgga cggcacgagc
     5521 gcgcgcggcc cgcaccgagc cgggcaccgt cgtgcccgac gaggtcctcg tggagatcgc
     5581 cgccaagcgc ccccgcaccc ggcccgagat cgacgccgtg gcggggctcg gcccggcccg
     5641 tcgcaaccgc ttcgccgacg agctgctggc cgtcgtggcc cgcgccgagc acgcccgatg
     5701 aggttcaccg tcgagcagcg cttcagcacc ggcgcggacg agacggcgcg ggcgttcgcc
     5761 gacccggacc tgtacgccgg cttcgcggcg ctgccgaagg tctcggtacc cgacgtcctc
     5821 tcctgcgagc gcaacggcga cctggtcgag ctgcgcatcc gctaccgctt cgccggccac
     5881 ctgtcctccg cggcgcgggc cgtcatcgac ccggcccagc tgacctgggt ggacgagtcc
     5941 acccacgacc tggcccggcg cctcgtccgg ttcgtgctcc gccccgacca ccacggcaac
     6001 cgcttccgct gctcgggcga ctaccgcatc gagccgcacg gcgaggggtg ccggcgccac
     6061 gcgaccatcg acctcaaggt gtcggccccc ttcgtcggcg gcgcggtgga gcgggcgatc
     6121 gagtccgggc tgcgggagca cctcgccgac gagaccgagg tggtggaggc atacctggcg
     6181 gcccgtcggt aggttcgcct ggtgcgcctg gccacctgga acgtgaactc gctcaaggcc
     6241 cgactcgagc gggtcgaggc ctggttggcc gaggcatctc ccgacgtgct ctgcgtgcag
     6301 gagaccaagc tggccgacga cgcgttcccg gccctggcgc tcggcgccct cggctacgac
     6361 ggcgtccacc acggccaggg gcggtggaac ggcgtggcga tcctgtcccg catcgggatc
     6421 agcgacgtga cccacggctt cgccgacggc ggcccgcccg acgacgaggc ccgcctgctc
     6481 accgcccggt gcggcaccac cgtggtggtc accgcctacg ccccgaacgg tcgcgccgtg
     6541 ggccacgagc agttccacta caagctggcc tggttcgacc ggctcgccgc ccacctcgac
     6601 gccgtggccg atccctccga cgacgtcgtc gtctgcggcg acctgaacat cgcaccggag
     6661 gaccgcgacg tctgggacgt cgacgccatc cacggcgcga cccacatcac cgaggacgag
     6721 cgccaccgct tccagcgcct cctcgactgg ggcctggtgg acgcgttccg ccgctgctac
     6781 cccgacgacg accggctgtt cacctggtgg gactaccggg ccggaaactt ccacaagcac
     6841 aagggcatgc gcatcgacca cgtcctcgtc agcgaaccgc tggcctcccg gctgtcgtgg
     6901 tccctggtgg accgcaacgc ccgcaagggc accggcccct ccgaccacgc cccgctgctc
     6961 atcgaggtcg cgtccgaggc ggtgccggca tgaacgacgc cgacgccgac gccggcggcc
     7021 aggccttcgg gccgaacagc ccgagctggc cgagccgtct cgccgagctg gcctcggagg
     7081 agcccacgcc gcgccggcgt gagctgcgcc gcctggccga cgccacgcga cgggtgctgc
     7141 acacgatggt catgaacggc gccgacgccg acacgctcgc cgcggccgcc gacgcgctcg
     7201 aggcagtggc cgacgacctc caccaccacg cccaacccag ccagtcgatc tacgaggggt
     7261 tcggcgaggc ggccaccagc gggaacccgc acggcttctt cgaccacagc ccgatgctcg
     7321 gcgtggccaa cccgatcgca ccgcccatcg tcctgcgcgg gatcgacgag cggaccatgg
     7381 agggcgcggc caccttcgga ccggcctacg agggcccacc cgggtgcgtg cacggcggct
     7441 acatcgcggc cgcgttcgac gaggtcctcg gcgccgtgca gtcgctgtcg ggccagccgg
     7501 gcatgaccgg caccctcatc gtgcgctacc gctcccccac accgctgcac gagccgctgc
     7561 ggttcaccgg acggttcgag ggcgtcgagg gtcgcaagat cctcaccacc ggcacgctcc
     7621 acgctggtga ccggctctgc gccgaagcgg aggggatctt catctccgtg gacttcggcc
     7681 ggatggccga gctgcgatcg cggcgggccg aacagcaggc ccgccgcgac gaccggacct
     7741 gacctaggag cgcttggtgg cccgcttgga ggccgccttc ttcgtggtgg acttcttggc
     7801 cggcgacttc ttggccgctg ccttcttcgt cgtcgccttc ttggccggcg acttcttggc
     7861 cgctgccttc ttcgtcgtcg ccttcttggc cgaggacgtc ttggccggcg acttcttggc
     7921 cgctgccttc ttcgtcgtgg ccttcttggc cgaggacttc ttggccgctg ccttcttcgt
     7981 cgtcgccttc ttggccggcg acttcttcgc ccggcttgcc ggcgcggact tcttggcggt
     8041 cgccttcttg gcgggcgccg ccttcttctt cgtcgcagcc ttcttggccg cagccttctt
     8101 cttgctcgct gccttcttgg tcgccatggg agacgtcgcc ttcctgtccg ttgtcttctg
     8161 gtccggtgct gccttcttcg ccgcggcccg tgcccgggtc ctcgatgcgg ccttcttcgc
     8221 gggtgcatcg gcttcggctg cggccgccgc tgcggcggcc tcggcttcgg cctgttgctg
     8281 ggcacggagc ttgcggcgct gggagacgga gagcttgcgg tctccgtcgt cgatgacggg
     8341 cgcagcagcc ggagctgcct cggccgtcgg cgcgtcctcg acctcgatcg cgtcgtcgcc
     8401 ggtgtcgatc tcgtcgtcgt cgggggcgtc gagcgccatg ccgggcatgg cgaggtcgat
     8461 gcctcgtcga ggggtgtcga tggccttgac cacgaactcg cgggtctcgc ccagctcgaa
     8521 gacctcgcgc gcgctgcggg gcggcgggtc ggagatggac ttgagcgaga tgtaggcgcg
     8581 ggtgccgtcg cagaggacgt aggcgccgtg gctggcgaac tgctcgatct cggcctcgat
     8641 caccgacccc accggatggg cgccgacgaa ctcgatgaac gggagcggct cgttgtaggg
     8701 ctcgggctcc ttgcccccgc gcttgcggcc agccggctcg tcggtcttgg ccttggcctt
     8761 ggaccgtccg gacgacttgg ccgacttcga cgaggacttc gaccgggacg acttggtcga
     8821 cttcgacgac gacttcgatg gacgcgagga ggcgggggtc ttcgacgcgg cggtcttgga
     8881 cccgcgccgc ttgcgcttgg cgtcggacac ggcccggcgg ctggtgggcc cgcgcaccgg
     8941 cgagcgggcg aggaagatcc acccgacgcc ggggacgtgc ttgcccccga gcagccggcc
     9001 ctcctggaag agccagtcgt aggtgccgtg gaactcctgg aaggagtcgt tggacaagat
     9061 gatggcgtcg gcgcgatcgg cgacctcgag gatgaaggca tcaccgcgac cgatcacacc
     9121 cgccgggggc gtgatgatct ggccgctgtt gaccttctcc tcgtactggt cgcgctcgga
     9181 ggcgtcgatg cggtgcccga aggtggcatc gacgatgacc gtcacgttgg cgtgatcgaa
     9241 ctcggcggtg accgcgtcca cggcctccag cagctgctgg aggctgggaa gggatcgacc
     9301 ttcggttgcg atgttggatc cgtcgacgac gagatgcgtg gttggcaaag tgtgacgcag
     9361 caggttcggt ggcgcagggc ccgcagctgc gatgccggac ccgcttcggc gtgaccctac
     9421 tggcgtgacg gccgccatcc gcggagcacc gtccgcgcgg cgtcatggta ggaaccccgt
     9481 cgtgcccgat cccgacgccc agatcaccgc cgcgctcttc gacttcggag gcgtgatcct
     9541 gtccagcccc ttcgatgcgt tcgccgacta cgagcgcgag cacggcctgc ccgagggctt
     9601 catccgcggg ctgaacgcca ccaacccgga tgacaacgcg tgggcgaaga tggagcgcaa
     9661 cgacgtcacc atccccgagt tctgcgagct gttcgaagcc gaggcccgag ccgccggtgg
     9721 cgagctcgac gccgccgcgg tgctggcccg gctgcggggg cagatccgac cggcgatggt
     9781 ggaggcggtg cgccggtgca aggcgcggct gaagaccgcg tgcctcacca acaacttcgt
     9841 ggcccccgat gccacccacc ccaacggccg catggacccg gagagcttcg acggggtcat
     9901 ggacctcttc gacgtcatcg tcgagtcgtc caaggcgggc tgtcgcaagc ccgacccccg
     9961 cttctacgag atcgcgtgcg aggagctcgg catcgagccg tccgaagccg tgttcctcga
    10021 tgacctcggc atcaacctca agccggccaa ggccatgggc atgaccacca tcaaggtcgt
    10081 cgaccccgac gaggccatcg ccgagctcga gcgggtggtc gggttcccgc tgcgctgacg
    10141 gggaacgggc gtccggcacg aaccggacgc gaccggcgct acggtgccgg ggatgtccgt
    10201 ccgcgcccgg cacaacgtcc agatctccgg cgtccccgac ggccagccgc tggtgttcgc
    10261 ccacggcttc ggctgcgacc agcacatgtg gcgccacgtc gcccctcgct tcgaggaccg
    10321 ctaccaggtc gtgctgttcg accacgtggg cgccggaggc tcccagaccg aggcctacga
    10381 ccccgagcgc cacgccagcc tcgacggcta cgccgaggac gtcgtcaccc tcgtcgaggg
    10441 cctcgacctg cacggcgtcg tgttcgtcgg ccacagcgtg agtgccatga tcgggatcct
    10501 cgccgctcgc cgtgcccccg agcgcttcgc ccacctcgtc ctcgtcggcc cgtcgccccg
    10561 ctacatcgac gatcccgaca gcggctacgt cggcggcttc agcgctcccg acatcgacga
    10621 gctcctcgcc tcgctcgagt cgaactacct cggctggtcc tcggcgatgg cgccggtcat
    10681 cgtcggcaac cccgagcggc ccgagctcgg cgtggagctg accgagagct tctgcaaggc
    10741 agaccccgag atccagcagc gcttcgcgaa ggccacgttc ctctcggaca accgcgccga
    10801 cctcgcgcac gtgccggtcc cgtgcctggt gctccagtgc cgtgacgacg tcatcgcacc
    10861 ggagtcggtc ggccgctacg tcgacgacca gctccccgag agcgagctcg tcatcctcga
    10921 cgccaccggc cactgcccga acctcagcgc gccggacgag accaccgccg ccatcgaggc
    10981 ctacctccgc cggcaccggg atcggtgagc gggctctccg ccgtgggcgc gtcggggccg
    11041 acccacggct acgtgcaggc gctgatcgag gacgacccgg ccgagctgta cgagacggcg
    11101 ccgtgcgggt acctgtcgac cgacccgtcg ggcgcgatcg tcaaggtcaa ccgcacgttc
    11161 ctcgactgga cggggtacgg gcgcgacgag ctcgtgggca gccgccggtt ccagtccctg
    11221 ctcgccgtcg gcgaccgcat cttctacgag acccacttcg cgccgtcgct cgccatgcag
    11281 ggcaccgtcc gcgagatcgc cgtcgaggtc gtgtgcgccg acggcgagcg cctcccggcg
    11341 ctcgtcaacg ccgtctccac ggacgctgtc ggcggccggc ccggccgcat ccgcatcgcc
    11401 gtgttcgacg cccgggagcg gcgctcgtac gagcgcgagc tgctgatcgc ccgcgagcgt
    11461 gccgagcagg cggccgccga ggcccgggcg ctcgccgaga ccctgcagcg cacgcttctg
    11521 cccctgtcgc cccccgagat cccgggcatg gacgtcggca ccgcataccg ccccgcaggc
    11581 gacggcagcg tggtgggcgg cgacttctac gacctgttcg agctgcgcga cggcacctgg
    11641 ggcgtcacgc tcggcgacgt ctgcggcaag ggtgcaccgg cggccatggt caccgcgctc
    11701 gcccggcaca ccgtccgcgc cctcgccgtg cgcagcgcca agccgagcga ggtgctgcgg
    11761 ggtctgcacg acgccatcgc ccgcagcgac gtcgacagct tctgcactgc gctctacctc
    11821 acggtgtgcg ccgaggacgg cgccctcgag gtgtgcatgt cggccggcgg ccaccacctg
    11881 ccgctgcggg tcggcgtcga cggcacggtc gccgaggtcg gccggcccgg gcacctgctc
    11941 ggcatgctcg agccgttgca cctccacgac gaggacgtcg ccgtcggtcc gggcgagaag
    12001 ctggtgctct acaccgacgg tgtcatcgag gccgcccgcg ccggtgactt cttcggcgag
    12061 ggacgactgc acgacgtcat ccgcgagcac cggggcgacg ccgcgcagga cctggccgac
    12121 gccatcgccg gcaccgccgt cgagttccag gagcaggaca cgcgcgacga catcgccgtc
    12181 gtggtgctgg gagctgcggc tagtacgtga gcgccttgtc caggatcatc gcctgctcct
    12241 ggtggtggat cttgttcgcg ccggtggccg gtgagccgga ctcgtaccgc gacacccgct
    12301 ggatcttgtg gctgtcgccg aagccctcgt acaaccggcg gctggcgaag ttccacgcac
    12361 ccatgttctc cggctcctcc tggagccaga tgagctcggt ggccttcggg tagcgctcga
    12421 gctcctcggc caggcgggca ccgggccacg ggtagacctg ctcgacccgc acgatggcga
    12481 cgggcacctg gcgctcgtcg cgggaggcga tggcgtcctg ggcgaccttg ccggtggcga
    12541 ggaccacccg ccgcacgtcg tcggtgtcga tggcaccgct ggccaccgcg gggtcgggca
    12601 ggacctcctc gaaggaaccg tgcgtgagct cctcgacgga ggaccgggcc ggcttggccc
    12661 gcagcagcga cttcggcgtg aacacgatca gcggacgacg cgcctcctgg tgcatctggc
    12721 gtcgcagcag gtggaagaac tggccggcgg tggtggcgtt gacgacctgg atgttgtcct
    12781 cggcgcacag cagcaggaag cgctccatgc gggccgagct gtgctcgggt ccctggccct
    12841 cgaagccgtg gggcagcagc atgatgagac cggagtcctg ctgccacttg tcggcggcgg
    12901 cgacgatgaa ctggtcgatg atgatctggg cgccgttgac gaagtcgccg aactgggcct
    12961 cccaggcgac catcgccttc gggttctcca ccgagtagcc gtactcgaag ccgagcgcgg
    13021 cgtactccga cagcagcgag tcgtagatcc agagcttgcc ctggtcgtcg gcgatgttgc
    13081 cgagcggcac gtactcggcg ccgttggtgt ggtcgtgcag cgtggcgtgc cggtgggcga
    13141 acgtgccccg gcgggtgtcc tggccggcca ggcggatgtc gatgccctcg agcagcaggg
    13201 tgccgaaggc cagggactcg gcgaggcccc agtcgacctc accggactcc cacatcttgc
    13261 ggcgggtgtc gaactgcttg gccagcttgg gatggaggtt gaagtcctcc ggcgtcgagt
    13321 cgagggcggc gaacacccgg tcgaggacct cgcggtcgac gccggtctcg atgtggggca
    13381 gcacgccgcg cgccgcgggc ggttcggcgg ccatgtgctc ctccggggag gagcgggtct
    13441 cgtcgagcgc gtcctggagc cgggcctgga agtcgtcgag ggcggcctcg gcctcctcga
    13501 gggacatgtg gccccgcttc acgagggact cggtgtacag cttgcggacc gaccggtgct
    13561 gatcgatctt ggcgtacatc atcggctgcg tgtagctggg gtcgtcgccc tcgttgtgac
    13621 cgtggcgccg gtagcagacc atgtcgatca cgacgtcctt gttgaaccgc tggcggaact
    13681 cgaaggcgag gcgggcggcg cggacgcagg cttcggggtc gtcaccgttc acgtggaaga
    13741 ccggtgcctg gatcatcttg gccacgtcgg tggagtagaa gccggagcgg ctggcctccg
    13801 gcgtggtcgt gtagccgatc tggttgttga cgatcaggtg caccgtgcca cccacgcggt
    13861 agccgcggat gtcggacatc tgcagggtct cgaccacgac gccctggccg gcgaaggccg
    13921 cgtcgccgtg gatgaggatc ggcaggacgc tgaacgcctc gggctggtcg atctggtcct
    13981 ggagggctcg gaccatgccg accaccaccg ggtccaccgc ctcgaggtgg ctcgggttgg
    14041 ccgccagctc gacggtgatc tcctgctcgt ggcgcgagca gtacacgccc tcctggccga
    14101 ggtggtactt gacgtcgcca ctgccctgca ccgacccctc gggcacgtag ccctcgaact
    14161 cgctgaacag ctggtcgagg tccttgccgg cgatgttcac cagcacgttg agccggccgc
    14221 ggtgggccat gcccatgatc gccttgtcca tgccctcggc cgcggcgcgc tcgagcaccg
    14281 cgtcgaggat cacgatggcg gactcgccgc cctcgaggcc gaagcgcttc tggccgacgt
    14341 acttggtggc caggaacttc tcgaacgcct cggcggcgtt gagccggccg agcacgtggt
    14401 gctgctcgtc ggggtcgagc gcgacctcga cgccctcgac ctgctcctgg atccaggcct
    14461 tctgctccgg gtcctggatg tgcatgtact cgatgcccag ggtccggcag taggcgtcgc
    14521 gcagcacctt gaggatctgg cgcagcgtca gcttctcccg cccggcgagg ccgccggtga
    14581 ggtactcgcg ctcgaggtcc cagatcgtga gcccgtaggt gagggggtcg agctcggtgt
    14641 gcatgttcgg ctcgcgccag cgcagcgggt cgaggtcggc catgaggtgg ccgcgcacgc
    14701 ggtacatgtt gatgagccgc tggacggcca tctgcttctc gcgccggtag gcctcctggt
    14761 ccaccgggcg cacgtcctgg cgccactgga cggcctcgta gggcacgtcc agggcccgga
    14821 agatctcgtc gtagaagccg tcctcgccga gcagcagctc gtggacgcgc ttgaggaaga
    14881 ccccggactc ggcgccctgg atgatgcggt ggtcgtaggt cgaggagatc gtgatgacct
    14941 tcgacaggcc gaggtcggcc agcgtctcgg ggtcggccgc ctgcatctcg gcgggccagt
    15001 cgatggagcc cacgcccacg atcaggccct ggccgggcat gaggcggggc acggactgga
    15061 ccgtgccgat ggtgcccggg ttggtgaggc tgaccgtgat gccggagaag tcgtccgggg
    15121 ccagcttgtt gttgcggacc ttgcggatca gctcctcgta ggagccccag aagccgcgga
    15181 agtcgagggt gtcggcgtcc ttcaccacgg gcacgagcag cgagcggctg ccgtcggcct
    15241 tctcgatgtc gacggcgatg ccgagcccga cgtggtcgtt gcgcacgatg cgcggcttgc
    15301 cgtcggcgcc ctcgacgtag ctgttgttca tcgccggcac cgtgtcggcg atggcccgga
    15361 cgacggcgta tccgatgagg tgggtgaagc tgaccttgcc acctcgggtg cggcccaggt
    15421 agccgttgat gaccgcccgg ttgacctcga gcagcttggc cgggacgttg cggtagctcg
    15481 tcgccgtcgg gaccgcgagg ctgcgctcca tgttctcgac gatgcgggcg ccgacgccgc
    15541 ggatgggctc gccgacctcg tcctcggcgg cctcggcctt cttctcgtcg gacttcttcg
    15601 cctcgggctc cggcgccggg gcgggcgccg gcttggccgg ggcgccggcg ccgttggtgg
    15661 ccgccggtga cgcggcgatg ggctcggcgt cggagtcgcg cttgtagtcg gcgaagaagt
    15721 cctgccagga ctcgctcacc gacgaggggt cggagaggta ctgctcgtac atctcgtcga
    15781 ccatccaggc gttcggtccg acgagatccg gggtctgctt ctcggccacc tggcgagcgt
    15841 accgccgggc tccggcggcg ccgttgcccg cggtcgaggg atcagccggc agcgaccagc
    15901 gagcggcacc gctcggcgtc gagcgggtgg ccggcggcgc cgaagcccga cgccgcccga
    15961 gcgaagtgct gcttcgccag cccggcgtcg ccggcagcga ggcggacgtg cccacggacc
    16021 tcctcgtggg cggcgtccca tgcaggcagt cgcatgacga cctgggcgag ccatgcgctc
    16081 gcctcctcgt accggagggc gcgatcgagc tcaccggcgg tggcacaggc gatggctgcg
    16141 ggcacggcga aggtgatccg gcaccccgga cacgtctcga gctggccctg cactgcgctc
    16201 tcggcttcgt cgacggcagc caacgcggcg tcgggatcgc ggcccgcgag ggtgatccgg
    16261 gtgccgtaga tccggtccag caggtggaag ccgatgtcgg tgacccgggc caggtccagc
    16321 gcctcgtcga ggaggtccca cgcctcccgg tcccgtcccc ggtggatggc gacctcggcg
    16381 agccgctgga gcgcgagggc ctcaccgacc gcgcccccca ccgcccggtg gaggcggccg
    16441 ccgtcgcgga ggtcgacctc ggcctcgtcc agccggccgg cgagcag
//
LOCUS       DATISU010000412        21296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_500866, whole genome shotgun sequence.
ACCESSION   DATISU010000412 DATISU010000000
VERSION     DATISU010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 21296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21296
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500866"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /gene="trxA"
                     /locus_tag="VLR27_16280"
     CDS             complement(<1..643)
                     /gene="trxA"
                     /locus_tag="VLR27_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HSP05071.1"
                     /translation="MAYVDVTDQTFQTEVIDRSTQVPVVVDLWAEWCGPCKTLGPTIE
                     KVVDATGGKVVLAKVDVDANRAIAQAFQVQSIPAVYAMRDGKVVDGFVGALPEHQVQA
                     WVDQLIPSAEESEVSKLVAAGDEASLRQALELEPGNEPAIEALAALLIDGSTNEGKAE
                     ALDLLARIPETDTTRHLAAQARMANPDVPADVDSVLAGLLPQVKGDEDAKQQYL"
     gene            769..1788
                     /locus_tag="VLR27_16285"
     CDS             769..1788
                     /locus_tag="VLR27_16285"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HSP05072.1"
                     /translation="MQGIEIEPVTDWYRANVPGVTPPLEFELIAGGHSNLTYRVTDAN
                     GRRTVLRRPPLGQVLASAHDMGREHRIIAALHDTDIPVAPPLGYVPDDSVNGAPFYVM
                     DFVDGHVLRTQDEALALSPEARANCGDSLVDVLARIHAVDVDEVGLGDLGRKEGYIER
                     QLKRWYGQWEKSKTRELPEIDEVHRILSADVPDQGPAAIVHGDYRLDNCMVDDQGDVI
                     AVLDWELCTLGDALADVGLLMVYWSEPGDRSPTLGAPTAAEGMRSRQELLDRYAAASG
                     RDLSHIDYYVAFGYWKLACIVEGVYARYIGGSMGTRDTSDFDGFKLQVEALAAQAKAA
                     VDRLA"
     gene            1794..2678
                     /locus_tag="VLR27_16290"
     CDS             1794..2678
                     /locus_tag="VLR27_16290"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HSP05073.1"
                     /translation="MSLYELHERPDLDAPVLLVALDGWIDAGGAAARAVESIVAQGDT
                     THVATFDADALLDFRARRPILHLVDGVNTGLTWSVTELHATTAPNGTDVLLLTGVEPD
                     HAWNAFADSVLGLAVELGCGMQIGLGAYPAAAPHTRPVQLSATASTDELAAQVGLVRG
                     TVDVPAGIEAVLEQRFAAMGLPAVGVWAQVPHYVAAMRYPAAAARLIEGLNLVAGTDF
                     GTEDLDVEIGAMRTRIDELVESNPQHVAMIHQLEEAYDQSLEAMSSDDSSLGGAPLPS
                     GDELAEELQRFLREQGDQ"
     gene            2722..3729
                     /locus_tag="VLR27_16295"
     CDS             2722..3729
                     /locus_tag="VLR27_16295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HSP05074.1"
                     /translation="MKIDGGIGLDLNTAGEQAKEAEDFGYDGIWVPETSRDPFLPLVL
                     ATEHTERLEIGTSIAVAFARSPMTLANTAYDLTEYSKGRFILGLGSQIKPHITKRFSM
                     EWSKPAARMREMVLAMHAIWDSWMTGAKLDFKGDFYTHTLMTPFFNPGPHEYGHPRVF
                     LAGVGPLMTEVAGEVCDGFFCHGFTTEKYLREVTLPALEAGRQKAGKTLEGFEICGPS
                     FVVTGNTEEELEAAKVGTRRQIAFYGSTPAYRPVLECHGWGELQTDLNRMSKEGKWEE
                     MGTLIDDEILETFAVVGEPEAIGPELQKRYGDVISRLSFYAPYAGNRDRWQRVLEDMR
                     G"
     gene            complement(3755..5071)
                     /locus_tag="VLR27_16300"
     CDS             complement(3755..5071)
                     /locus_tag="VLR27_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05075.1"
                     /translation="MILAHGVGSRADLPVEPWLFAYSAAFALLISFAALRLLWPRPRL
                     AAAATGTPVPAVLGTVASVLGVVFKGLALALFATTLVAAWFGEDAVSANLAPTALYIA
                     LWIGMQVASAVLGDVWRRINPLWTVASALDRIRGRDPETSTATGWWASHWPAALGLLA
                     FHWLELAYYEPASLTAVAAFLSVYTVAVLVAASWFGAGWVRTGDGFAVLFGLLAALSP
                     LHRDEAGRLRLRLPGSGLAAVDLGPGTLGVILVVLGGTTFDGVTRTTWWSDIAAGRRE
                     WDLTVVSTIGLLLTIATVAIAYLVAIRVLGVLAKDDADLVDQARRWGSSLIPIVLGYS
                     IAHYFSLLVFEGQSFLALLSDPLGSGRDLFGTAQDTIDFTVVTADQIAYTQVAAIVIG
                     HIAGVIAAHDKAVERYPHRTAVLSQYPLLAVMVAYTVSGLLLLLNA"
     gene            complement(5068..6198)
                     /locus_tag="VLR27_16305"
     CDS             complement(5068..6198)
                     /locus_tag="VLR27_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05076.1"
                     /translation="MGRPLFRRAAAILAAALLVAACGDDDTTTTADDTVVEDTTTAEM
                     ADGHGHGEGIEVGDADEIPTVSITATPDAKSGINLQVEATGITFAPEHASSDHVPGEG
                     HAHVYVDGEKYGRVYGEWTHLDVEAGEHEIRVDLNANDHSPLLVDGEPVDDVVTVEAP
                     EPPSGHGHMAGFEADAPFPTVALTVTEDPKSGWNVQIETTDYTFAPDKASTMETTSGE
                     GHAHLYVDGRKVTRMYGEWFHVDEDLAAGEHEFRVELSANDHSPYLADGAPIEAVQTV
                     TVEGMPEDGPSADDADQVIEVAIAGDRVENGGRHEVAVGDTVALVITSDVDDEIHVHG
                     YDLYADIGPAGPATVLVEATIPGVWEVELHDSGLLLVELAVS"
     gene            complement(6258..6812)
                     /locus_tag="VLR27_16310"
     CDS             complement(6258..6812)
                     /locus_tag="VLR27_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05077.1"
                     /translation="MGPRAVAISMLAVSGVAVGHIVGYAVAHPETAAREAALGGHAYL
                     PSAAAVAIPLGVVAALVWAIRTSRQLGLAGAIPFRTLAVTQLAVFAVQEIGERIAVGE
                     PASSVLTERGVWFGLLAQVAVAYLITRSVDAVRRAVHLLTGGGRVLRDVLCPPVSFVP
                     VVVRVQRAVAVSVGLRAPPVVGSR"
     gene            complement(6876..8567)
                     /locus_tag="VLR27_16315"
     CDS             complement(6876..8567)
                     /locus_tag="VLR27_16315"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP05078.1"
                     /translation="MISRAPRASQDTLATRVAAGAARGHDVTFVSGDVHVTVPWSDVH
                     EDATAMAAAMQQRGIRPGDHVALLGPTTRSLVTGIQAVWLAGATIVVLPLPMRMGSLD
                     EFVAQTRQRILDADVSLVVADPDLAAFITPEPGDPPIAVYDELATDASFTAPVPDPGA
                     NAILQFTSGSTSDPKGVVLPHRAVCANLDAIAEAAELAVDDDVLVSWLPLYHDMGLVG
                     ILSLAMTTGTRMVLGAPQDFLASPARWMQWMSQYGGTATAGPNFSYVLATRALRRAAR
                     EQQQLDLSKLRVVLNGAEPIDPHTVEDFLDAGAPFGMPRGSAFPAFGMAEVAIAGTFP
                     PPGRGLVVDTVDRRVLETERYAAPADPDADNARSFALLGRAVPGLEIRIVGPDDGREL
                     REREVGELEIRGTSVCAGYYGRPELNDELFHDDWLRTGDLGYLVGGELVLCGRIKDVI
                     IVAGRNVFPEDIERACNDIEGVRAGNVIAFGVETAKGKEGMVVVAEARPPEGTTDLSP
                     VRADVQHRVQAVAGIPAKDVVLVAPGTLPKTSSGKLQRALCRARYLDGQLELVHA"
     gene            8648..9121
                     /locus_tag="VLR27_16320"
     CDS             8648..9121
                     /locus_tag="VLR27_16320"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSP05079.1"
                     /translation="MTPITPRPLEWIDSAPVRASRSRRIAQPTSVVWAAIADHAGWTE
                     WFPRLVAVTPGAVADAVGGTRTVDLGAAAVEEEFLAWEPERRFAFTVTGSTRPGLRSM
                     VEDIRLTPDGDRACTVTYTMGVDPVGGKLLRPVAAPLLRKVIADGLAGLAVHVRG"
     gene            9133..10074
                     /locus_tag="VLR27_16325"
     CDS             9133..10074
                     /locus_tag="VLR27_16325"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HSP05080.1"
                     /translation="MRASGGQRIEWREVDPDVRHAIEAHVGAPVVDARNQAGGFSPGL
                     AARCTLADGGRVFIKAVSPEQNPQACRIHRREAEVAAQLPADLPVPRLLHVVDDGRWV
                     ALVFEEIEGVQPDEPWTHGQLDVVLPALQRFAERATPTPAPGLQTVEARYGTVFHGWR
                     QIASGDVDASSYGPDVGAAVDRLATLEATWTEAAAGSTLLHTDLRADNLLLTPTGEVV
                     LVDWPWACTGAAFFDLVLLLPSVGLSGGPDPATVIEAHRLFGDVDPDALVSVFAALAG
                     FFVRQSLDPAPPGLPTIRSFQRAQAEVALGWLLPHIS"
     gene            10133..11014
                     /locus_tag="VLR27_16330"
     CDS             10133..11014
                     /locus_tag="VLR27_16330"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HSP05081.1"
                     /translation="MRFNRRARLDSDQVRTRKGGAAAVGGGAGIVGILVVLALQFLGG
                     GGGDLGGALGALEGTRTGGQGASSEPIEGCETGADAERSQECRIVAVVNSIQDLWSTQ
                     LDGYQPAVTNFFTGGVTTGGCGSASSAVGPFYCPADQQIYIDLGFFDDLESRFGASGG
                     DFAEAYVLAHEYGHHVQNLIGIRAERGSGPESDAVRQELQADCFAGVWANHATTTPDP
                     ATGEILILELTQQDIDEALSAAAAVGDDRIQEAATGQVNPHTWTHGSAEQRQQWFLTG
                     YETGDPNQCDTFASDEL"
     gene            11097..11693
                     /locus_tag="VLR27_16335"
     CDS             11097..11693
                     /locus_tag="VLR27_16335"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HSP05082.1"
                     /translation="MKLRTLAVGAAAAGILALTAAPAQAAHAEATTTVGLNGANEVPE
                     KGDPNGSGKIDITVFGPRVINDGNRATVAFDDVYYLCYDLVTRNIDGVAAAHIHEVDR
                     GEGQERTNPRKLTGGVVVDLQPGATETSDSVKFKNDAGQVVAFEDATSTCVTALDGSD
                     DEVIDEMTREPREYYVNVHNEEFPAGAIRGQVNFETDR"
     gene            complement(11743..14259)
                     /gene="leuS"
                     /locus_tag="VLR27_16340"
     CDS             complement(11743..14259)
                     /gene="leuS"
                     /locus_tag="VLR27_16340"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HSP05083.1"
                     /translation="MAADTASNEVADGRVPDYDPQTVEAKWQQRWRDEGTYEIDNDDP
                     RPKYYVLNMYPYPSGPAHMGHVRNYTFGDLIVRYKTMQGHGVLSPMGFDSFGLPAENA
                     AIQSGIHPREFTEERIPQLTSSITRIGAVYDWRRTVASHTPEYIRFTQWIFLRFLERG
                     LAYKRKAAVNWCPGCQTVLANEQVLADGTCERSGDLVEARDLEQWFFKITDYAQQLLD
                     DLELVDWPERVVTMQRNWIGRSEGAQFSMPVVDAEGEPHESAARVEVYTTRPDTAFGM
                     TYAVLAPEHELALTVTTPEHRDEVEAFIAKARSTSEMDRLSSEGAIDKRGVFTGSYLQ
                     NPFNGEPVPLYLADYVLVTYGTGAIMAVPGEDQRDWDFAKAYDLPIVRTVQPPEDFDG
                     EAYTGTGSAINSAGTTITGDELDLNGLEMAEAKSKAIDWLTANGIGQDRVNYRLRDWL
                     LSRQRFWGCPIPIVYCTKCAGIDGSGAVPVPDDQLPVIAPDDVEFLPTGESPLKHHEG
                     FLRTTCPSCGGEAMRETDTMDTFVDSSWYFLRFADPTNEDLPFSQEAVERWLPVDQYI
                     GGVEHAILHLMYARFFTKALADIGVAPSDLREPFQRLFTQGMIRLGGAKMSKSKGNLV
                     APEEILDREGADALRLGQLFVGPPADDVDWEGVGIEGTHRFIGRLWRLAHPVSDAVAE
                     ATGDAAVAVDKEAHRLIARVSDEYERWSYNTAVAAFMEFTNLLYKQGRTDFAVDSLLL
                     LLAPMAPHVTAELWELRHGTHVHEQAWPTHDPDMLAVDRVTMVVQVKGKVRDRIEVSP
                     DISEDEARSLALASEAVQGHLDGEPTKVIVRVPNIVNIVP"
     gene            14383..14455
                     /locus_tag="VLR27_16345"
     tRNA            14383..14455
                     /locus_tag="VLR27_16345"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14416..14418,aa:Ala,seq:ggc)
     gene            14546..15430
                     /locus_tag="VLR27_16350"
     CDS             14546..15430
                     /locus_tag="VLR27_16350"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP05084.1"
                     /translation="MADPVIPLPDRLVELPGGRRVALDDRGDDDGSPVLYCHGSPDSR
                     FARHPDDSLAAIARVRLLAADRPGIGHSDVDPDATPTSVADDLAAVLDHLGIERVGVL
                     SWSTGAIHALAFAGTHPDRCRRVVLAAPLVPADAYDDPTVLDGSDDARRLFADAHRGM
                     DPGDVGAELAPWLVPPVIDDATAREMMAGSITALADIPGADDQLALAVRASVAAGMTG
                     VERDIAAQATRLDALLDRITATASVHVGADDAVTPPAMARWIGRRLDVRPQVHDGIGH
                     ELAIRRWADLLQEAAAEG"
     gene            complement(15434..15880)
                     /gene="rsfS"
                     /locus_tag="VLR27_16355"
     CDS             complement(15434..15880)
                     /gene="rsfS"
                     /locus_tag="VLR27_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HSP05085.1"
                     /translation="MTDAEQTATPAPASPGLADDLLDPTFPIAAARAAFAKGATDILV
                     LQVGDVLAITDYFVIASASNPRLVRTIVQDCEEAVAAAGGPKALRVEGLAESEWVLAD
                     HGTFVVHVFHQDARQYYELERLWSDVPQIAWEDPDLPAAAASAGTR"
     gene            complement(15873..17210)
                     /locus_tag="VLR27_16360"
     CDS             complement(15873..17210)
                     /locus_tag="VLR27_16360"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HSP05086.1"
                     /translation="MTGEAGDGDPDIDDDEREARLQALLSVRVTARPDELGPGPLEGR
                     RRRTTASDGDGDAETGAGDSGSRRRIRLGTVLATLVLLGLFATGAGLVYAGTRIIRSS
                     TEGEVVAEQTDPASPGYEAIVSPTPTMLLVHDVEGTVASLTVLTLPEADGGGGGVVLV
                     PTRTVAEIPMLGFAPIEAAYDLATLDLQVQVVGDVLNAGIGEAAVVDAERWEGLVAPV
                     APLTIDNPDDLVVDGEVRFSAGTIELQAEDVGPYLAASHDGESDLARLFRHHTFWTAW
                     LDAVAASGDPSAVPGELESGVGRFVRALAAGERVVETLPVEPTTVEGFDDEPTFLPET
                     EEIAALVAELVPLPRSPAFGVRPRVRVLNGTTELDEARAVALDLPPVGVEVVAVGNAR
                     SLDEETTSIAYVNPSHEEAAQDIRDLLGVGEVFVDPRPSDVVDITVTLGADHD"
     gene            complement(17207..17800)
                     /gene="nadD"
                     /locus_tag="VLR27_16365"
     CDS             complement(17207..17800)
                     /gene="nadD"
                     /locus_tag="VLR27_16365"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HSP05087.1"
                     /translation="MAAARIGVFGGTFDPPHVGHLVTAVNVRHDLRLDRLLLVVANEP
                     WQKIGSRPITPASDRLAMVEAAVAGVAGLEACALEIQRGGHSYTADTLTELAGLHPGA
                     ELFLVLGADAAAGLPTWERVEEVRSLASLVIVDRPGEPPPVVPDGWDWTHVEVPRLEV
                     SSTDLRDRVADGRPLEYLLPRDVVACVRERRLYRGST"
     gene            18021..18854
                     /locus_tag="VLR27_16370"
     CDS             18021..18854
                     /locus_tag="VLR27_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSP05088.1"
                     /translation="MSDSVGQYLNEIGMVPLLTAEEERELSQIIEKGREAQERVDAGE
                     KKTREERRAIADAAAAKDRFIRANLRLVVSIARRYPLPPSMELLDLIQEGNLGLEHAV
                     DKFDWRKGFKFSTYATFWIRQAIGRALDQKASLVRLPGDRSASLRAALRHASGEGTEL
                     DDEHSRLHRLTTPTSLDRTVGDEADGSELIDLLADNKPGPETLTLKAAEDEFVTGLLD
                     VLEPRARYAVEQRFGLNDGRTRSYREVGEELGVTAEAARRLVKRAVGTVREEAGTRGA
                     A"
     gene            19039..20379
                     /locus_tag="VLR27_16375"
     CDS             19039..20379
                     /locus_tag="VLR27_16375"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HSP05089.1"
                     /translation="MTQWTPTSWRTKPAAQQPEWPDEGALEAVLKELGSYPPLVFAGE
                     VRDLTAQLAQVAAGEAFLLQAGDCAESFDSFSADAIRDKLKVILQMAVVLTYSSGVPT
                     VKVGRIAGQFAKPRSSSTETVDGTELPSFRGHIVNDVGFTSASRQADPGRLLQAYHQS
                     SSTLNLLRAFTKGGFADLGRVHQWNQEFVASSREGERYEALAHDIDRTLRFMKACGID
                     TVETPYLHEVDFFTSHEALLLGYEEALTRQDSLTDDWYDCSAHMLWIGERTRQLDGAH
                     VEFLAGVRNPIGCKVGPTATADDVLGLCEALNPDRIPGRLTLISRMGADKIAELLPPL
                     LEATRDGEHPVVWACDPMHGNTFTSESGRKTRHFEDVVREVAGFFAAHRDAGTWPGGI
                     HIELTGDDVTECLGGAEEIGDADLGNRYETMCDPRLNGRQSIDLAFRVSELLRG"
     gene            20536..>21296
                     /locus_tag="VLR27_16380"
     CDS             20536..>21296
                     /locus_tag="VLR27_16380"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HSP05090.1"
                     /translation="MQRHEIVLDPEQAADIAKFETQLAAYLDGTLAEDAFRVFRLTNG
                     IYGQRQGGTNQMVRVKIPYGKIEPAQLDMMAHIADTYSRGWGHITTRQNVQFHFVPLE
                     RIPDVMRDLAVVGMTSREACGDTNRNVQGCHLAGACPHEVLDISAWAQATHEHFLRNP
                     LVQRLPRKFKINFSGCATDCGQAMFNDVGVIAVTHQHEDGSLEAGFRVFVAGGLGANP
                     HPALALEEFTPRHRLLETIEAILRVFNNHGFRDNKL"
BASE COUNT         3109 a         7778 c         7442 g         2967 t
ORIGIN      
        1 ccaggtactg ctgcttggcg tcctcgtcgc ccttcacctg gggcaggagg ccggccagca
       61 ccgagtccac gtcggcgggg acgtcggggt tggccatccg ggcctgtgcg gcgaggtggc
      121 gggtggtgtc cgtctccggg atgcgggcga gcaggtccag cgcctcggcc ttgccctcgt
      181 tggtgctgcc gtcgatgagc agggcagcca gcgcctcgat ggccggctcg ttcccgggct
      241 cgagctcgag cgcctggcgc agcgacgcct cgtcgccggc cgcgaccagc ttcgacacct
      301 cggactcctc ggccgacggg atcagctggt cgacccacgc ctggacctgg tgctcgggca
      361 gcgcaccgac gaagccgtcc acgaccttcc cgtcgcgcat ggcgtagacg gcagggatcg
      421 actgcacctg gaaggcctgg gcgatggctc ggttggcgtc cacgtcgacc ttggccagca
      481 cgaccttgcc gcccgtggcg tcgaccacct tctcgatggt ggggcccagg gtcttgcacg
      541 gcccgcacca ctcggcccag aggtcgacga cgaccggcac ctgggtggag cggtcgatca
      601 cctcggtctg gaacgtctgg tcggtcacgt cgacgtatgc catggcggcc aacctaccgg
      661 cgaggtccga tctcgccgcc tccggcgccc gggcgggtca acgtcgccgg gggccgctca
      721 ctaggttggc gaacccacac cgcgagagac ggaggccaag tggcggacgt gcagggaatc
      781 gagatcgaac cggtcacgga ctggtaccgg gcgaacgtgc ccggggtgac gccacccctg
      841 gagttcgagc tgatcgccgg cggccactcg aacctcacgt accgggtcac cgacgcgaac
      901 ggccgccgca cggtgctgcg ccggccgccg ctcggccagg tcctggccag cgcccacgac
      961 atgggccgcg agcaccgcat catcgccgcc ctccacgaca ccgacatccc cgtcgccccg
     1021 cccctcggct acgtccccga cgactcggtc aacggcgcgc ccttctacgt catggacttc
     1081 gtcgacggcc acgtgctgcg cacccaggac gaagcgctcg cgctgtcgcc cgaggcgcgg
     1141 gcgaactgcg gcgactccct cgtcgacgtg ctcgcccgca tccacgccgt ggacgtcgac
     1201 gaggtcggcc tgggcgacct cggccgcaag gagggctaca tcgagcgcca gctcaagcgc
     1261 tggtacggcc agtgggagaa gtccaagacc cgcgagctgc ccgagatcga cgaggtccat
     1321 cggatcctca gcgccgacgt gcccgaccag gggccggccg ccatcgtcca cggcgactac
     1381 cgcctcgaca actgcatggt cgacgaccag ggcgacgtga tcgcggtgct cgactgggag
     1441 ctgtgcacgc tcggcgacgc cctcgcggat gtcgggctgc tgatggtgta ctggtccgag
     1501 ccgggcgacc gctcccccac cctcggggcg ccgacggcag ccgagggcat gcgcagccgc
     1561 caggagctgc tcgaccgcta cgccgccgca tcgggtcgcg acctgtccca catcgactac
     1621 tacgtcgcct tcggctactg gaagctggcc tgcatcgtgg agggcgtcta cgcccggtac
     1681 atcggtggct ccatgggcac gcgggacacg tcggacttcg acggcttcaa gctccaggtc
     1741 gaggccctcg ccgcgcaggc gaaggccgca gtggatcggc tggcctgacc gccgtgtccc
     1801 tgtacgagct gcacgagcgt cccgacctgg atgccccggt cctgctcgtc gcgctcgacg
     1861 gctggatcga cgccggcggg gccgcggcac gggccgtgga gtccatcgtc gcccagggcg
     1921 acacgaccca cgtggccacc ttcgacgccg acgcgctgct cgacttccgg gcccggcgcc
     1981 cgatcctgca cctcgtggac ggcgtgaaca ccggtctcac ctggtcggtc accgagctgc
     2041 acgcgacgac ggcaccgaac ggcaccgacg tgctgctgct caccggcgtc gagcccgacc
     2101 acgcctggaa cgccttcgcc gactcggtgc tcgggctcgc cgtcgagctc gggtgcggga
     2161 tgcagatcgg cctcggcgcc tatcccgcgg ccgcgccgca cacccgaccc gtccagctct
     2221 ccgccaccgc ctccaccgac gagctcgccg cccaggtcgg cctcgtccgg ggcaccgtcg
     2281 acgttcccgc cggcatcgag gccgtcctcg agcagcgctt cgccgccatg ggcctccccg
     2341 ccgtcggcgt ctgggcccag gtgccgcact acgtcgcggc catgcgctac ccggctgcgg
     2401 cggctcgcct gatcgagggg ctgaacctgg tggccggcac cgacttcggc accgaggacc
     2461 tcgacgtcga gatcggcgcc atgcgcaccc gcatcgacga gctggtcgag tccaaccccc
     2521 agcacgtcgc gatgatccac cagctcgagg aggcctacga ccagtcgctc gaggcgatga
     2581 gcagcgacga ctcctcgctc gggggtgcac cgctgccctc gggtgacgag ctggccgagg
     2641 agctgcagcg cttcctccgg gagcagggcg accagtagcc acggaacttg accggtcagt
     2701 caagaaccgt atgatccggt catgaagatc gacgggggca tcggactcga cctcaacacg
     2761 gccggcgagc aggccaagga ggccgaggac ttcggctacg acggcatctg ggtgccggag
     2821 acgtcacggg acccgttcct gccgctcgtc ctggccaccg agcacaccga gcgcctcgag
     2881 atcggcacct ccatcgccgt ggccttcgcc cgctccccga tgacgctggc gaacacggcc
     2941 tacgacctga ccgagtactc gaagggtcgc ttcatcctcg gcctcggcag ccagatcaag
     3001 ccccacatca ccaagcgctt ttccatggag tggtccaagc cggccgcccg catgcgagag
     3061 atggtgctgg ccatgcacgc catctgggac agctggatga ccggggcgaa gctcgacttc
     3121 aagggcgact tctacaccca cacgctgatg acgccgttct tcaacccggg cccgcacgag
     3181 tacggccacc cgcgggtgtt cctcgccggc gtcggcccgc tgatgaccga ggtcgccggc
     3241 gaggtctgcg acgggttctt ctgccacggc ttcaccaccg agaagtacct gcgcgaggtc
     3301 accctgcccg cgctcgaggc cggccgccag aaggccggca agaccctcga gggcttcgag
     3361 atctgcggcc ccagcttcgt ggtcaccggc aacaccgagg aggagctgga ggcggccaag
     3421 gtcggcaccc ggcgccagat cgcgttctac ggctcgactc ccgcctaccg cccagtcctc
     3481 gagtgccacg gctggggcga gctccagacc gatctcaacc gcatgtccaa ggagggcaag
     3541 tgggaggaga tgggcacgct catcgacgac gagatcctgg agacgttcgc ggtcgtgggc
     3601 gagcccgagg ccatcggccc cgagctccag aagcgctacg gcgacgtcat ctcccggctc
     3661 agcttctacg ccccctacgc cggcaaccgc gaccggtggc agcgggtcct cgaggacatg
     3721 cgcggctgat cgttccgcgg tcgctgccag aagctcacgc gttcagcagc aggagcaggc
     3781 cggagacggt gtaggccacc atcacggcca gcagcgggta ctggctgagc acggccgtgc
     3841 ggtgcgggta gcgctcgacc gccttgtcgt gggcggcgat caccccggcg atgtggccga
     3901 tgacgatcgc cgccacctgg gtgtaggcga tctggtcggc cgtgaccacg gtgaagtcga
     3961 tggtgtcctg cgccgtgccg aacaggtcgc gccccgagcc gagcggatcc gagagcaggg
     4021 ccaggaagct ctgcccctcg aacaccagca gcgagaagta gtgggcgatc gagtagccga
     4081 gcacgatcgg gatgagcgac gacccccagc ggcgggcctg gtccacgagg tcggcgtcgt
     4141 ccttggccag cacgccgagc acccggatgg cgaccaggta ggcgatggcc accgtggcga
     4201 tcgtcagcag caggccgatg gtgctgacca cggtcaggtc ccactcccgc cggccggcgg
     4261 cgatgtcgct ccaccacgtg gtgcgggtga cgccgtcgaa ggtggtgccc ccgaggacca
     4321 cgaggatcac gccgagcgtg ccgggtccca gatcgaccgc ggcgagaccg gagccgggca
     4381 ggcgcaggcg cagccgcccg gcctcgtcgc ggtggagcgg ggacagggcg gccagcagac
     4441 cgaacaggac ggcgaacccg tcgccggtgc gcacccagcc ggcgccgaac cagctcgccg
     4501 ccaccaggac ggcgacggtg tagacgctga ggaacgcggc cacggccgtc agcgaggccg
     4561 gctcgtagta ggcgagctcg agccagtgga aggcgaggag ccccagcgct gccggccagt
     4621 gcgacgccca ccatccggtc gccgtcgagg tctccgggtc ccgccctcgg atgcggtcga
     4681 gggcggacgc cacggtccac agcgggttga tgcgccgcca cacgtccccg agcaccgcgc
     4741 tcgccacctg catgccgatc cacagcgcga tgtacagggc ggtcggcgcc aggttggccg
     4801 acacggcgtc ctcgccgaac cacgccgcca cgagcgtcgt ggcgaacagc gcgagggcga
     4861 gacccttgaa gacgacgccg agcaccgatg ccaccgtgcc cagcaccgcc gggacgggcg
     4921 tcccggtcgc ggcggccgcc agccgcgggc gcggccacag cagccgcagc gcggcgaagg
     4981 agatgagcag ggcgaaggcc gccgagtagg cgaacagcca cggttcgacg ggcaggtcgg
     5041 cgcggctgcc gaccccgtgg gcgaggatca cgagaccgcc agctcgacga ggagcaggcc
     5101 gctgtcgtgc agctcgacct cccagacgcc ggggatggtg gcctcgacca ggacggtggc
     5161 cggcccggcc ggaccgatgt cggcgtacag gtcgtagccg tgcacgtgga tctcgtcgtc
     5221 cacgtcgctc gtgatcacca acgccaccgt gtcgccgacc gcgacctcgt ggcggcctcc
     5281 gttctcgacg cggtcgccgg cgatcgccac ctcgatcacc tggtcggcgt cgtccgccga
     5341 gggcccgtcc tcgggcatgc cctccaccgt cacggtctgg accgcttcga tgggtgctcc
     5401 gtcggccagg tagggcgagt ggtcgttcgc cgacagctcg acccggaact cgtgctcgcc
     5461 ggcggcgagg tcttcgtcca cgtggaacca ctcgccgtac atgcgagtga ccttgcggcc
     5521 gtccacgtag aggtgggcgt ggccctcccc cgaggtggtc tccatcgtcg aggccttgtc
     5581 gggggcgaag gtgtagtcgg tggtctcgat ctgcacgttc cagccggact tcggatcctc
     5641 ggtgaccgtg agcgccaccg tcgggaaggg cgcatcggcc tcgaagcccg ccatgtgccc
     5701 gtggccgctc ggcggctccg gggcctcgac ggtcacgacg tcgtcgacgg gctcgccgtc
     5761 gacgaggagc ggcgagtggt cgttggcgtt gaggtcgacg cggatctcgt gctcgccggc
     5821 ctcgacgtcg aggtgcgtcc actcgccgta gacccggccg tacttctcgc cgtccacgta
     5881 cacgtgggcg tggccctcgc cggggacgtg atcggaggat gcgtgctcgg gggcgaaggt
     5941 gatgccggtg gcctccacct gcaggttgat gccggacttg gcatccgggg tggcggtgat
     6001 cgagacggtg gggatctcgt cggcgtcgcc gacctcgatg ccctcgccgt ggccatggcc
     6061 gtcggccatc tcggcggtcg tggtgtcctc gacgacggtg tcgtcggcgg tcgtggtggt
     6121 gtcgtcgtcg ccgcatgcag cgacgagcag cgcggcggcg aggatcgcgg ccgcgcgacg
     6181 gaacaggggt cgtcccatgt tgtgtctcct cggttgttcg tctgtggtcg gtggaccggc
     6241 gcgacgccgg tcccgggtca gcgggagccg accaccggcg gggcacggag cccgacggag
     6301 accgcgacgg cacgctggac gcgcacgacc acggggacga aggagacagg agggcagagg
     6361 acgtcgcgca ggacgcgacc gccgccggtg agcaggtgca ccgcccggcg gacggcgtcg
     6421 accgagcgcg tgatgaggta cgcaaccgcc acctgggcga ggagaccgaa ccacacccca
     6481 cgttcggtga ggacgctgga tgccggttcg ccgaccgcga tccgctcgcc gatctcctgc
     6541 acggcgaaga cggcgagctg ggtgacggcg agcgtgcgga acgggatcgc cccggccagc
     6601 ccgagctggc gactggttcg gatcgcccac accagcgcgg cgacgacacc gagcgggatg
     6661 gcgaccgcgg cggcggacgg gaggtaggcg tgaccgccga gcgcggcctc gcgtgcggcc
     6721 gtctccgggt gtgcgaccgc gtagcccacg atgtggccga cggcgacgcc ggagaccgcc
     6781 agcatcgaga tcgccacggc gcgggggccc atgggcgcga cgctagcccc gccccgcccg
     6841 acttccggat tcgaccaggg gcgcgtcccg cccgctcagg cgtggaccag ctcgagctgc
     6901 ccgtcgaggt agcgcgctcg gcacagggcg cgctgcagct tgccgctgct cgtcttgggc
     6961 agggtgcccg gtgccacgag caccacgtcc ttggccggga tgccggcgac cgcctgcacc
     7021 cggtgctgca cgtcggcccg cacgggcgag aggtcggtgg tgccctcggg cggacgggcc
     7081 tcggccacca cgaccatgcc ctccttgccc ttggcggtct ccacgccgaa ggcgatgacg
     7141 ttgccggctc ggacgccctc gatgtcgttg caggcccgct cgatgtcctc ggggaacacg
     7201 ttgcgaccgg cgacgatgat cacgtccttg atccgcccgc acagcacgag ctcgccgccg
     7261 accaggtagc cgaggtcgcc ggtgcgcagc cagtcgtcgt ggaacagctc gtcgttcagc
     7321 tccgggcggc cgtagtaccc ggcgcacacc gaggtgccgc ggatctcgag ctcgccgacc
     7381 tcgcgctcgc gcagctcgcg gccgtcgtcg ggtccgacga tgcggatctc cagaccgggc
     7441 acggcgcgac ccaggagggc gaagctgcgg gcgttgtccg cgtcgggatc ggccggggcc
     7501 gcgtagcgct cggtctcgag gacccgacgg tccacggtgt ccacgacgag gccccgtccc
     7561 gggggcggga aggtgccggc gatggccacc tcggccatgc cgaaggcggg gaacgccgaa
     7621 cccctcggca tgccgaacgg cgcaccggcg tcgaggaagt cctcgaccgt gtggggatcg
     7681 atgggctcgg cgccgttgag cacgacgcgg agcttcgaca ggtccagctg ctgctgctcc
     7741 cgtgccgccc ggcggagggc gcgggtggcg aggacgtagg agaagttcgg cccggccgtg
     7801 gcggtgccgc cgtactgcga catccactgc atccagcggg ccggcgaggc gaggaagtcc
     7861 tgcggggcgc cgagcaccat gcgggttccc gtggtcatgg cgagggagag gatcccgacc
     7921 aggcccatgt cgtggtagag cggcagccag gacaccagga cgtcgtcgtc gaccgccagc
     7981 tctgcggcct cggcgatcgc gtcgaggttg gcgcacacgg cccggtgcgg cagcacgacg
     8041 cccttcgggt ccgatgtgga gccgctggtg aactgcagga tcgcgttggc gccgggatcg
     8101 ggcaccggcg cggtgaagga tgcgtcggtc gccagctcgt cgtagacggc gatcggcgga
     8161 tcgcccggct cgggggtgat gaaggcggcg aggtcggggt cggcgacgac gagggagacg
     8221 tcggcgtcca ggatccgctg ccgggtctgg gcgacgaact cgtcgagcga ccccatccgc
     8281 atggggagcg gcaggaccac gatcgtggcg ccggccagcc acaccgcctg gatgccggtg
     8341 accagcgacc gggtcgtcgg tccgaggagg gcgacgtggt cgccgggtcg gatcccccgc
     8401 tgctgcatgg ccgcggccat ggccgtggcg tcctcgtgga cgtcggacca cgggaccgtg
     8461 acgtggacgt cgccggacac gaaggtgacg tcgtgtccgc gggccgcgcc ggcggcgact
     8521 cgggtggcga gggtgtcctg ggaagcgcgg ggcgcacgcg agatcatggg cacgcggttt
     8581 cgactacccc gatcgcctcg cggttacccc gtttcgcacc gggatgccac cgcgcgaggc
     8641 tccggccatg acgccgatca cgccccgccc gctcgagtgg atcgacagcg cccccgtgcg
     8701 cgcctcccgc agccgacgga tcgcccagcc gacctcggtg gtgtgggccg ccatcgccga
     8761 ccacgccggg tggaccgagt ggttcccccg cctcgtggcg gtgaccccgg gagcggtcgc
     8821 cgacgccgtc ggtggcacgc ggacggtcga cctcggtgcc gccgccgtcg aggaggagtt
     8881 cctggcctgg gagcccgagc ggcgcttcgc cttcaccgtc accggctcga cccgccccgg
     8941 cctgcgctcg atggtcgagg acatccggct gacccccgac ggcgaccgag cctgcaccgt
     9001 cacctacacg atgggggtcg accccgtcgg cggcaagctg ctccgacccg tcgccgcacc
     9061 gctcctccgc aaggtgatcg ccgacggcct cgccggcctc gccgtccacg tgcggggctg
     9121 acgggcgccc tcgtgcgggc cagcgggggc cagcggatcg agtggcgcga ggtcgatccc
     9181 gacgtccggc atgcgatcga ggcgcacgtc ggggcgccgg tcgtcgacgc ccgcaaccag
     9241 gctggcggct tctcgcccgg actggcagcg cgctgcacgc tggccgacgg agggcgggtg
     9301 ttcatcaagg cggtgtcgcc cgagcagaac ccgcaggcgt gccggatcca ccggcgcgag
     9361 gccgaggtgg ccgcgcagct cccggccgac ctcccggttc cccggctgct ccacgtggtc
     9421 gacgacgggc gctgggtggc gctggtcttc gaggagatcg agggtgtgca gcccgacgag
     9481 ccgtggacgc acgggcagct cgacgtcgtc ctgcccgccc tccagcgctt cgccgagcga
     9541 gcgacgccga ccccggcacc cgggctgcag accgtcgaag cccgctacgg cacggtgttc
     9601 cacgggtggc gccagatcgc ctccggcgac gtcgacgcct cgtcctacgg ccccgacgtc
     9661 ggcgcggcgg tcgaccggtt ggcgacgctg gaggccacgt ggacggaggc cgctgcgggg
     9721 agcacgctcc tccacaccga cctgcgggcg gacaacctgc tcctcacccc gaccggggag
     9781 gtcgtcctgg tcgactggcc gtgggcgtgc acgggcgccg ccttcttcga cctggtcctg
     9841 ctgctgccct ccgtcggcct ttccggcggt cccgacccgg cgacggtgat cgaggcgcac
     9901 cggctgttcg gcgacgtcga tcccgacgcg ctcgtgtcgg tcttcgccgc gctcgccggc
     9961 ttcttcgtcc gccagtcgct ggatccggcg ccaccggggc tgccgacgat ccgctccttc
    10021 cagcgggccc aggccgaggt cgcgctcggg tggctcctcc cccacatctc ctgaggtggg
    10081 acgatctgct ggcgcaggtc cggacacgag gtcgacgggg taggggtggc cgatgcgatt
    10141 caaccgacgg gcccggctcg attccgacca ggtgcgcaca cgcaagggcg gcgcggcggc
    10201 cgtcggcggt ggtgccggca tcgtcggcat cctcgtggtg ctggcgctcc agttcctcgg
    10261 cggcgggggc ggcgacctcg gcggcgcgct cggtgcgctc gagggcaccc gcaccggcgg
    10321 ccagggggcg tccagcgagc cgatcgaggg ctgcgagacc ggcgccgacg ccgagcggag
    10381 ccaggagtgc cgcatcgtcg ccgtggtcaa cagcatccag gacctgtggt ccacccagct
    10441 ggacggctac cagcccgcag tcaccaactt cttcaccggc ggcgtcacca cgggcggctg
    10501 cggttcggcc agctcggcgg tcgggccctt ctactgcccg gccgatcagc agatctacat
    10561 cgacctcggc ttcttcgacg acctcgagtc ccgcttcggg gcgtccggtg gcgacttcgc
    10621 cgaggcctac gtcctggccc acgagtacgg ccaccacgtc cagaacctca tcggcatccg
    10681 agccgagcgg ggcagcgggc cggagagcga cgccgtgcgc caggagctgc aggccgactg
    10741 cttcgccggc gtgtgggcca accacgccac caccacgccg gacccggcca ccggcgagat
    10801 cctgatcctc gagctgaccc agcaggacat cgacgaagcg ctgagcgcag cagctgccgt
    10861 cggcgacgac cgcatccagg aggccgccac cggccaggtc aacccccaca cctggaccca
    10921 cgggtcggcc gagcagcgcc agcagtggtt cctcaccggc tacgagaccg gtgacccgaa
    10981 ccagtgcgac acgttcgcct ccgacgaact gtagaaaagc cgcgcaacgg tcatacgggt
    11041 gccgggctgg ctccggagaa ggggggaacc agccacctca ccaggagatg cccctcatga
    11101 agctccgcac cctcgccgtc ggcgccgccg ccgccggcat cctcgccctc accgctgccc
    11161 cggcccaggc cgcacacgcg gaggccacga ccaccgtcgg cctcaacggt gccaacgagg
    11221 tccccgagaa gggcgacccc aacggctcgg gcaagatcga catcacggtc ttcggacccc
    11281 gggtcatcaa cgacggcaac cgggccacgg tcgcgttcga cgacgtgtac tacctctgct
    11341 acgacctcgt cacccggaac atcgacgggg tggctgcggc ccacatccac gaggtcgatc
    11401 gtggcgaagg ccaggagcgg accaaccctc gcaagctgac cggtggggtc gtcgtcgacc
    11461 tccagcccgg tgccaccgag acctccgatt cggtgaagtt caagaacgat gcgggccagg
    11521 tcgtcgcgtt cgaggacgcc acgagcacct gcgtcaccgc cctcgatggc agcgacgacg
    11581 aggtcatcga cgagatgacc cgcgagccgc gcgagtacta cgtgaacgtc cacaacgagg
    11641 agttccccgc cggcgcgatc cgtgggcagg tcaacttcga gaccgaccgc tgatcccgca
    11701 gatcgaccgt ggccgggtcc tcggacccgg ccacggtcgc gcctacggga cgatgttgac
    11761 gatgttcggg acgcggacga tgaccttggt cggctcgcca tcgaggtgac cctggaccgc
    11821 ctcgctggcc agcgccagcg agcgggcctc gtcctcggag atgtccggcg agacctcgat
    11881 tcggtcccgc accttgccct tgacctgcac gaccatcgtc acgcggtcga cggccagcat
    11941 gtccgggtcg tgggtgggcc acgcctgctc gtggacgtgg gtgccgtggc gcagctccca
    12001 cagctcggcc gtgacgtggg gggccatcgg cgcgaggagc aggagcaggg agtccacggc
    12061 gaagtcggtg cgaccctgct tgtacagcag gttcgtgaac tccatgaacg ccgccacagc
    12121 cgtgttgtag gaccagcgct cgtactcgtc agacacgcgg gcgatgaggc gatgggcctc
    12181 cttgtcgacg gccaccgctg cgtcgccggt ggcctccgcc accgcatcgg agacggggtg
    12241 ggccaggcgc cacaaccgcc cgatgaaccg gtgggtcccc tcgatgccga cgccctccca
    12301 gtcgacgtcg tccgccggcg gtccgacgaa gagctgaccg aggcgcagcg cgtcggcacc
    12361 ctcccgatcg aggatctcct cgggcgcaac caggttgccc ttcgacttgg acatcttggc
    12421 tccgccgagg cggatcatgc cctgggtgaa gaggcgctgg aacggctccc gcaggtcaga
    12481 cggtgcgaca ccgatgtcag ccagggcctt ggtgaagaac cgggcgtaca tcaggtggag
    12541 gatggcgtgc tcgaccccgc cgatgtactg gtcgaccggc agccagcgct ccacggcctc
    12601 ctgcgagaac ggaaggtcct cgttggtcgg gtcggcgaag cgcaggaagt accacgacga
    12661 gtccacgaac gtgtccatcg tgtcggtctc gcgcatggcc tcgccgccgc agctcggaca
    12721 ggtggtgcgc aggaacccct cgtggtgctt cagcggcgac tcgccggtcg ggaggaactc
    12781 gacgtcgtcg ggagcgatca ccggcagctg gtcgtccggc accggcacgg cgccgctgcc
    12841 gtcgatccct gcgcacttgg tgcagtacac gatcgggatc gggcaccccc agaagcgctg
    12901 gcgggacagc aaccagtccc gcaggcggta gttcacccgg tcctggccga tgccgttggc
    12961 ggtcagccag tcgatggcct tggacttggc ctccgccatc tccaggccgt tcaggtccag
    13021 ctcgtcgccg gtgatggtgg tgccggcgct gttgatggcc gaccccgtgc ccgtgtacgc
    13081 ctcgccgtcg aagtcctccg gcggctgcac ggtgcgcacg atgggcaggt cgtaggcctt
    13141 ggcgaagtcc cagtcgcgct ggtcctcacc gggcacggcc atgatcgccc cggtgccgta
    13201 cgtgaccagc acgtagtcgg cgaggtacag cgggaccggc tcgccgttga acgggttctg
    13261 caggtacgag ccggtgaaca ccccgcgctt gtcgatggcg ccctcggacg agaggcgatc
    13321 catctccgac gtcgagcggg ccttggcgat gaaggcctcg acctcgtcgc ggtgctccgg
    13381 cgtggtgacc gtgagggcca gctcgtgctc gggggcgagg acggcgtagg tcatgccgaa
    13441 ggcggtgtcg gggcgggtcg tgtacacctc gacgcgggcg gcggactcgt gcggttcacc
    13501 ctcggcgtcc acgacgggca tcgagaactg ggcgccctcg gagcggccga tccagttccg
    13561 ctgcatggtc acgacccgct cgggccagtc gaccagctcc aggtcgtcga gcagctgctg
    13621 ggcgtagtcg gtgatcttga agaaccactg ctccaggtcg cgggcctcga ccaggtcgcc
    13681 cgaccgctcg cacgtgccgt ccgccagcac ctgctcgttg gccagcaccg tctggcagcc
    13741 cgggcaccag ttcaccgccg ccttgcgctt gtacgccagg ccccgctcca ggaagcgcag
    13801 gaagatccac tgcgtgaacc ggatgtactc gggcgtgtgg ctggccacgg tgcggcgcca
    13861 gtcgtagacc gcgccgatgc gggtgatcga cgaggtcagc tgcgggatcc gctcctcggt
    13921 gaactcccgg gggtggatgc cggactggat ggcggcgttc tcggccggca acccgaagga
    13981 gtcgaacccc atgggcgaca gcacgccgtg gccctgcatc gtcttgtagc ggacgatcag
    14041 gtcaccgaag gtgtagttgc gcacgtggcc catgtgcgcc ggccccgacg ggtacgggta
    14101 catgttcagc acgtagtact tcgggcgcgg atcgtcgttg tcgatctcgt aggttccctc
    14161 gtcgcgccac cgctgctgcc acttcgcctc gacggtctgg gggtcgtagt cgggcacgcg
    14221 cccgtccgcg acctcgttcg aagcggtgtc tgcggccatg gcccggcatc gtaccgaggg
    14281 cccctgcggc ctccgattcc ggttcttccg ccgaggcgac accgatttcc gtcggcgacg
    14341 gcgacgcgat gtacgctggc cgctccgcga tgcggaccct acggggctat agctcagttg
    14401 gtagagcgct tccatggcat ggaagaggtc aggggttcga ttccccttag ctccacggca
    14461 acccttcggc cgcagggcgg cctcgggttg cttgacggat gcttcgcatc cgtctgcccg
    14521 aggcttgcgt gcctcgtctc cgaccatggc tgatcctgtg atccccctcc cggaccggct
    14581 ggtcgagctg ccgggtggtc gacgtgtcgc tctcgacgat cggggcgacg acgacggcag
    14641 ccccgtcctg tactgccacg gctctccgga ctcccggttc gcccggcacc ccgacgactc
    14701 gctcgctgcg atcgcccgcg tccggctgct cgccgccgat cgccccggca tcggccactc
    14761 cgacgtcgat cccgacgcca cgccgacctc ggtcgccgac gacctggcgg ccgtcctcga
    14821 ccacctcggc atcgagcggg tcggggtcct gtcgtggtcc accggcgcca tccacgccct
    14881 cgccttcgcc ggcacccacc ccgaccggtg ccggcgggtc gtgctcgccg ctcccctggt
    14941 gccggccgac gcctacgacg acccgaccgt gctcgacggc agcgacgacg cccgccgcct
    15001 gttcgccgac gcccaccggg gcatggatcc cggcgacgtc ggcgccgagc tggccccctg
    15061 gctcgtcccg ccggtcatcg acgacgccac ggcacgcgag atgatggccg gctcgatcac
    15121 cgccctggcc gacatccccg gggcggacga ccagctggca ctggccgtgc gcgccagcgt
    15181 cgccgcgggg atgaccgggg tcgagcgcga catcgccgcc caggcgaccc ggctcgacgc
    15241 gctgctcgac cgcatcaccg cgaccgcgtc cgtccacgtc ggcgctgacg acgcggtcac
    15301 gcccccggcg atggcgcggt ggatcggtcg gcggctggat gtccgaccgc aggtgcacga
    15361 cggcatcggc cacgagctgg ccatccgtcg ctgggccgac ctcctgcagg aggcggcggc
    15421 cgagggctga gcgctaccgg gtgccggcgg aggccgccgc ggccggcaga tccgggtcct
    15481 cccaggcgat ctgggggacg tcggaccaga gacgctcgag ctcgtagtac tgccgggcgt
    15541 cctggtggaa cacgtgcacc acgaacgtgc cgtggtcggc caggacccac tcggactcgg
    15601 ccaggccctc gacccgcagc gccttggggc cgcccgccgc cgcgacggct tcctcgcagt
    15661 cctgcacgat ggtgcggacg aggcgggggt tggaggccga ggcgatcacg aagtagtcgg
    15721 tgatcgccag cacgtccccg acctgcagca ccaagatgtc ggtggcaccc ttcgcgaacg
    15781 ccgctcgcgc cgccgcgatc ggaaaggtcg ggtcgagcag gtcgtcggcg aggccgggcg
    15841 aggcgggggc gggtgtggcg gtctgctcgg catcagtcat ggtcggcgcc aagggtaacc
    15901 gtgatgtcga ccacatcact cggtcgaggg tccacgaaca cctcaccgac cccgagcagg
    15961 tcccggatgt cctgtgctgc ctcctcgtgg gacgggttca cgtaggcgat gctggtggtc
    16021 tcctcgtcga gcgagcgggc gtttcccacg gcgacgacct cgaccccgac cggtggcagg
    16081 tcgagcgcca ccgccctggc ctcgtcgagc tccgttgtgc cgttgagcac ccgcacccgc
    16141 ggccgcacgc cgaacgccgg gctgcggggc agcggcacga gctccgcgac gagcgcggcg
    16201 atctcctccg tctcgggcag gaaggtgggc tcgtcgtcga acccctcgac cgtcgtcggc
    16261 tcgaccggca gggtctccac gacccgctca cccgctgcga gggcccggac gaagcgaccg
    16321 accccgctct ccagctcccc cggcaccgcc gacgggtcgc cggatgccgc caccgcgtcc
    16381 agccacgccg tccagaacgt gtggtgccgg aagagtcggg ccaggtcgga ctcaccgtcg
    16441 tggctcgccg cgaggtacgg gccgacgtct tccgcctgca gctcgatcgt gccggcggag
    16501 aaccgcacct ccccgtcgac gacgaggtcg tcggggttgt cgatcgtgag gggtgcgacg
    16561 ggcgcgacca gcccctccca ccgctcggcg tcgacgacgg ccgcctcgcc gatgccggcg
    16621 ttgagcacgt cgcccacgac ctggacctgc agatcgagcg tggccaggtc gtaggccgcc
    16681 tcgatgggcg cgaagcccag catcgggatc tcggcgaccg tgcgcgtcgg caccaggacc
    16741 acgccgccgc cgccaccatc ggcctccggc agggtgagca cggtgagcga tgccaccgtt
    16801 ccctccacgt cgtggaccag gagcatggtc ggggtcgggc tgacgatcgc ctcgtagccg
    16861 ggtgaggccg ggtcggtctg ctcggcgacg acctcgccct cggtgctgga gcggatgatg
    16921 cgggtcccgg cgtacacgag gccggcgccg gtggcgaaca gcccgagcag cacgagggtg
    16981 gcgaggacgg tgcccaggcg gatgcggcgg cgcgatccgg agtcgccggc acccgtctcg
    17041 gcgtcaccgt ccccgtccga tgcggtcgtg cgccggcggc ggccttcgag gggtcccggt
    17101 ccgagctcgt cggggcgcgc cgtgacgcgc accgacagca gcgcctgcag gcgcgcttcc
    17161 cgttcgtcgt cgtcgatgtc cgggtcgccg tcgcccgcct cacccgtcac gtggacccac
    17221 gatagagccg gcgctcccgc acgcaggcca ccacgtcacg gggcagcagg tactcgagcg
    17281 ggcgaccatc ggccacgcgg tcgcgcaggt cggtcgagga cacctccagg cggggcacct
    17341 ccacgtgggt ccagtcccag ccgtcgggca ccaccggcgg gggttcgccg ggccggtcca
    17401 cgatcacgag cgacgccagc gagcggacct cctcgacccg ctcccacgtg ggcagcccgg
    17461 cggcggcgtc cgcgccgagc accaagaaga gctcggcacc ggggtgcagc ccggccagct
    17521 cggtgagggt gtctgcggtg tagctgtggc cgccgcgctg gatctcgagg gcgcaggcct
    17581 cgaggcccgc caccccggcc acggcggcct cgaccatggc gaggcggtcc gatgccggcg
    17641 tgatcggccg gctcccgatc ttctgccacg gctcgttcgc cacgaccagg agcagacggt
    17701 ccaggcgcag gtcgtggcgc acgttgaccg cggtgaccag gtgcccgacg tggggcgggt
    17761 cgaaggtccc cccgaacacc ccgatccgcg ccgctgccat ccggcgacga tattcgaccg
    17821 gcgcgagcgg accgcagcgg ccacactggc cggttccccc ctggccgagc catcccgacg
    17881 acgacgccgg gatgttctcg ctgtcacccc catcgggtac cgatgtacgg acgtgggttc
    17941 gaaccccctc ggacccctgt gactggagtc acgagcccgg tcggaatcct ccacccgcaa
    18001 ccgccgttgg aacgagtgtc atgagcgact ctgttggaca gtatctgaac gagatcggga
    18061 tggtgcccct cctcaccgcc gaggaggagc gcgagctctc ccagatcatc gagaagggcc
    18121 gggaggccca ggagcgcgtg gacgcgggtg agaagaagac ccgcgaggag cgtcgagcca
    18181 tcgccgacgc cgccgccgcc aaggaccgct tcatccgagc gaacctgcgg ctcgtggtgt
    18241 ccatcgcccg tcgctacccg ctgcccccct cgatggagct cctggacctg atccaggagg
    18301 gcaacctggg cctcgagcac gccgtcgaca agttcgactg gcgcaagggc ttcaagttct
    18361 cgacctacgc caccttctgg atccgccagg ccatcggccg ggccctcgac cagaaggcca
    18421 gcctggtccg cctccccggc gaccgctcgg cgagcctgcg ggctgcgctg cgccacgcct
    18481 ccggcgaggg caccgagctc gacgacgagc actcccgcct gcaccggctg accacgccca
    18541 cctccctcga ccgcaccgtg ggtgacgagg ccgacggctc cgagctcatc gacctcctgg
    18601 ccgacaacaa gcccggtccc gagaccctca cgctcaaggc cgccgaggac gagttcgtca
    18661 ccggtctgct cgacgtcctc gagccacggg cccgctacgc cgtcgagcag cgcttcggcc
    18721 tcaacgacgg ccgcacccgc agctaccgcg aggtcggcga ggagctcggc gtcaccgccg
    18781 aggccgcccg ccgcctggtg aagcgggccg tgggcaccgt ccgcgaagaa gccggcaccc
    18841 gcggagcagc ctgacgcccc gcttcggcag ggcccccacg tcgccacggc gaacgatcca
    18901 cccaccacca agacagacgc cctcccggta cctccccctt cccccgccgg gttgcgcgat
    18961 ctgcagcgtt cggaccgctt cccccggcgg tccgaactgc tttttgccca cccgcgagga
    19021 cgtgcgaggc ttgacgacgt gacccagtgg accccgacct cctggcggac caagcccgcc
    19081 gcgcagcagc ccgagtggcc cgacgagggc gcgctcgagg ccgtgctgaa ggagctgggc
    19141 agctatcccc cgctggtgtt cgccggggag gtccgcgacc tcaccgccca gctcgcccag
    19201 gtggccgccg gcgaggcgtt cctgctgcag gccggcgact gcgccgagtc cttcgactcg
    19261 ttctccgccg acgccatccg ggacaagctc aaggtcatcc tccagatggc ggtcgtgctc
    19321 acgtactcgt cgggcgtgcc caccgtgaag gtgggacgca tcgccggcca gttcgccaag
    19381 ccccgctcga gcagcaccga gaccgtcgac ggcaccgagc tgccctcgtt ccgcggccac
    19441 atcgtcaacg acgtcgggtt cacctccgcc tcccgccagg ccgatcccgg ccggctcctg
    19501 caggcgtacc accagtcgag ctccacgttg aacctgctgc gggccttcac caagggcggc
    19561 ttcgccgatc tcggccgggt ccaccagtgg aaccaggagt tcgtcgcgtc gtcgcgcgag
    19621 ggcgagcgct acgaggccct cgcccacgac atcgatcgca ccctgcgctt catgaaggcg
    19681 tgcggcatcg acaccgtgga gaccccgtac ctgcacgagg tcgacttctt caccagccac
    19741 gaggcgctgc tgctcggcta cgaggaggcc ctcacccggc aggactccct caccgacgac
    19801 tggtacgact gctcggccca catgctctgg atcggcgagc gcacccgcca gctcgacggc
    19861 gcgcacgtgg agttcctggc cggggtgcgc aacccgatcg gctgcaaggt ggggcccacc
    19921 gccaccgccg acgacgtcct cgggctgtgc gaggccctca acccggatcg catcccgggc
    19981 cgcctcacgc tcatctcccg gatgggcgcc gacaagatcg ccgagctgct ccctccgctg
    20041 ctcgaggcga cgcgcgacgg cgagcacccc gtggtgtggg cgtgcgatcc catgcacggc
    20101 aacaccttca cctcggagtc cggccgcaag acccgccact tcgaagacgt cgtgcgcgag
    20161 gtggccggct tcttcgccgc ccaccgcgat gcgggcacct ggcccggggg catccacatc
    20221 gagctcaccg gcgacgacgt caccgagtgc ctcggcggtg ccgaggagat cggggacgcc
    20281 gacctcggga accggtacga gacgatgtgc gatccgcgcc tgaacggtcg ccagtcgatc
    20341 gatctggcgt tccgggtctc cgagctcctc cggggctagc agccgcgggc cgagcggcac
    20401 ccagcagaca tctgcgcgga ttctgccgga caatcgggaa tgttgatctg gtcggtcggg
    20461 tttgacacac tgccgtcgtg caatacctga ccgatgtggt caggtttccc ggacacccga
    20521 ggggatcccc cagacatgca gcgccacgag atcgtgctcg accccgagca ggccgccgac
    20581 atcgccaagt tcgagaccca gctcgccgcc tacctcgacg gcacgctggc cgaggacgcc
    20641 ttccgggtgt tccgcctcac gaacggcatc tacggccagc gccagggcgg cacgaaccag
    20701 atggtccggg tcaagatccc ctacggcaag atcgagccgg cacagctgga catgatggcc
    20761 cacatcgcgg acacctacag ccgcggctgg gggcacatca ccacccgcca gaacgtgcag
    20821 ttccacttcg tgccgctgga gcgcatcccg gacgtgatgc gcgacctcgc cgtggtcggg
    20881 atgaccagcc gcgaggcctg cggcgacacc aaccgcaacg tgcagggctg ccacctggcc
    20941 ggcgcctgcc cccacgaggt gctcgacatc tccgcctggg cccaggccac ccacgagcac
    21001 ttcctgcgca acccgctcgt gcagcgcctc cctcggaagt tcaagatcaa cttctccggc
    21061 tgcgccaccg actgcggcca ggccatgttc aacgacgtcg gcgtcatcgc cgtcacccac
    21121 cagcacgagg acggctccct cgaggctggc ttccgggtgt tcgtggctgg cggcctcggc
    21181 gccaaccccc accccgccct cgccctcgag gagttcaccc cccggcaccg cctcctcgag
    21241 acgatcgagg ccatcctgcg ggtgttcaac aaccacggct tccgcgacaa caagct
//
LOCUS       DATISU010000413         6923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_594404, whole genome shotgun sequence.
ACCESSION   DATISU010000413 DATISU010000000
VERSION     DATISU010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594404"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="VLR27_16385"
     CDS             complement(<1..1131)
                     /locus_tag="VLR27_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HSP05091.1"
                     /translation="MRKFPDPPPGRPPGPVMSPDQRFGGRWPWLWAVVGAALLLSWLL
                     TQPSANEEDEVDYAEFRELVISGDVAEVEIDQGSGRIDGTLASGESFAAQGPPGGIPD
                     ADTALLDEHEVERNYVAESSNPLGSILLYLLPFVLIIGVLVWLSRRATNQLTGASAFT
                     RSRARVHRSARTDTTFADVAGYETVKDEISEVVDFLRDPSKFEEIGARIPKGILLVGP
                     PGTGKTLFARAVAGEAGVPFISVTGSEFLEMFVGVGAARVRDLFATARKETPSILFVD
                     EIDAIGRKRGTGLGGGHDEREQTLNQLLAEMDGFETTEGIVVLAATNRPDILDAALLR
                     AGRFDRQVVIPLPTAGERLAILEVHARGKKLAADVDLDLVARG"
     gene            complement(1219..1671)
                     /locus_tag="VLR27_16390"
     CDS             complement(1219..1671)
                     /locus_tag="VLR27_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05092.1"
                     /translation="MHPIRVSGRVPVEAGTQRRHLLWGSCPCGGKSRAERRRKVARGG
                     TDSAREITRGVHHAQQARRQRHARNFLEARDQLGQGEAVEAQIALQAAVQMHRWDTVR
                     VQLGDKIGDRVDHQTFGAIPDAIDRSHVVTVRRLPVPRQGRKTPGRPG"
     gene            1666..4068
                     /locus_tag="VLR27_16395"
     CDS             1666..4068
                     /locus_tag="VLR27_16395"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSP05093.1"
                     /translation="MHVRLLGEDGAVTDLTYGQLRHQARALAAGLLDAGVAPGESVAL
                     MLPTGTDYFVSFMGVVLAGAVPVPIYPPARPAQLEEHLERQSRILDNARAALLITVPE
                     ARRVSRLLRSGVESLRGIRTPGELLTARQTTLPVPGPDDIALLQYTSGSTGSPKGVVL
                     THRQLLANITAMARAARVTPDDVFVSWLPLYHDMGLIGAWLGSLHVGMPLVSMSPLSF
                     LVRPVRWLQVIDEHRATISAAPNFAFELCLRKADDTELRALDLSCWRLAFNGAEAVHP
                     TTIRRFTARLAPSGLRPETLMPVYGLAEAAVGLAFPPVGRPPLIDRIDRSEFVASGRA
                     RPIDDDQLPVDVVDFVACGRPLPGYELRVVDHTDRELPERHEGHLHFRGPSATTGYFR
                     NAAATRAMFHDDWLDTGDLGYLADGDLYVTGRVKDLIIRAGRNLHPDELEQTIGRILG
                     IRRGCVAVFAAPDPTAATERLVVVAETRETDAETLTTIRDRVAAATIALVETPPDDVV
                     LAPPGTVPKTSSGKVRRAETRARYESGTLNPQARAVWRQASRYALGRLPVRGRRIRRS
                     VGDALHTALGWAVLAAIGLPLGALLTVLPGARLRWTLTTWAARSALRLTGTTVHVHGE
                     LPPPGPAVIVANHASWIDGLVLTAVIPGRIRFVAGEVFADQALTGSVLRRIDTVFVER
                     WDREQGVADAAHLAELTHAGARLAVFPEGGLSPLPGLRPFHLGGFAAAAAGAVPVIPV
                     AVRGSRWVLRPGQRHLRRGAIDVFIGDPIEPVGDDWHAAIELRDAARAHILRHGGEPD
                     LA"
     gene            complement(4084..4524)
                     /locus_tag="VLR27_16400"
     CDS             complement(4084..4524)
                     /locus_tag="VLR27_16400"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSP05094.1"
                     /translation="MAVDGLAHLTEDECWRFVEHRHLGRVGVIEFHRRPLVYPVNYVI
                     HRRSVIFRTTPGTKLVAASLGAHAVIEVDEIDENSHSGTSVMVHGTLHEVTEPAERER
                     LRAVGVHPWAPGDRDHFVRLEPERVSGRRIPPTVPGDGLGADAG"
     gene            4689..>6923
                     /locus_tag="VLR27_16405"
     CDS             4689..>6923
                     /locus_tag="VLR27_16405"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HSP05095.1"
                     /translation="MPTLSTVTPGPHSSTIGLTSTAAAARLAVDGANKLPPPVERSAL
                     RRLVAQFVHFFALMLWVAAALAFVAGIPELGAAIALVIVLNALFAFFQESRAGQAAAR
                     LRALLPMQVTVVRDGRRQQVDSTQVVVGDVLALEGGDRIPADGTVLVAHGLLVDTALL
                     TGESEPERMAEGDDISAGTFVVEGEALALVSATGGGTRLADIAAMTTATDSPASPLTI
                     ALHRVVRTVALIAVSVGASFFIIMLAVDRPASDGLVLAIGVTVALVPEALLPTVTLSL
                     AWGAEQMAHRRVLVRNLEAVETLGSTTFICTDKTGTLTHNQMTVVDVWTPTATATIEE
                     PGYSPDSPVLVAGDRSALEALARAAVRCSTGYVHRVQGAWQPHGDPMEVAIDVFAHRL
                     GLDTEGDRALDVARARFPFDPRRRRMSVVVGDELLVKGAPDAVLPRCDDTEGAAIALD
                     RMMAAGLRVLAVSRRPVGPAVPVSADEAETGLELLGLVAMEDPPRLEVRDALAACRRA
                     GVRIAMVTGDHPGPARTIADQVGLRRPDDPVVVGHALPEDDDALGALIDHDGCVVARA
                     TPEDKVRIARALRARGHVVAMTGDGVNDGPSLHEAADLVLLDDAFASIVAGVEQGRAT
                     YGNMRRFLAYHLTDNVAELTPFLVWALSGGNFPLALGVMQILALDLGTDTLSAVALGA
                     EPPGKHLLDGPPVKGRLMNHSVVRRSFGLFGPLVAALTMAAFLVSLFVSGWRPGEVFP
                     TGPAL"
BASE COUNT          964 a         2641 c         2276 g         1042 t
ORIGIN      
        1 gccccgggcg acgaggtcca gatcgacgtc ggcggcgagc ttcttcccac gggcgtggac
       61 ctccaggatc gccagtcgct cccccgcggt cggcaggggg atgacgacct gacggtcgaa
      121 ccggcccgca cgcagcagcg ccgcatcgag gatgtccggt cggttcgtgg ccgcaaggac
      181 gacgatgcct tctgtcgtct cgaagccgtc catctcggcg aggagctggt tgagcgtctg
      241 ctcccgctcg tcgtggccgc caccgagccc ggtgccgcgc ttgcgaccga tcgcatcgat
      301 ctcgtccacg aacaggatcg agggcgtctc cttgcgcgcc gtggcgaaca gatcacgaac
      361 acgagcggcg ccgacaccca cgaacatctc caggaactct gaccccgtga ccgagatgaa
      421 cggcaccccc gcctcgccgg ccaccgcccg ggcgaagagg gtcttgccgg tgccgggcgg
      481 gccgaccagc aggatcccct tggggatgcg ggcgccgatc tcctcgaact tggacggatc
      541 gcgcaggaag tcgacgacct cgctgatctc gtccttcacc gtctcgtacc cggcaacatc
      601 cgcgaacgtc gtgtcggtcc gtgcactgcg gtggacgcga gcccggctgc gcgtgaacgc
      661 cgacgcaccg gtgagctggt tcgtcgcacg acgcgagagc cacaccagca ccccgatgat
      721 cagcacgaac ggcagcaggt acaacaggat gctgccgagc gggttggagc tctcggccac
      781 gtagttgcgc tcgacctcgt gttcgtcgag cagtgccgtg tcggcatccg ggatgccccc
      841 cggcggtccc tgcgcagcga acgactcgcc cgaggcgagg gtgccatcga tcctcccact
      901 gccctggtcg atctccacct ctgcgacatc accggagatg accagctccc ggaactcggc
      961 gtagtcgacc tcgtcctcct cgttcgccga gggctgggtc aacaaccacg agagcagaag
     1021 ggcggcgccg acgaccgccc acagccaggg ccaacgaccc ccgaagcgct ggtccggtga
     1081 catcaccggc ccgggaggcc gaccgggtgg ggggtcaggg aacttcctca cctgctcgat
     1141 gctcctgcgg ggcgttcgct gccacgaggt cacatggtga ctgtcgacga gaccatgccc
     1201 gggcggaaga ggcgaaggtc accccggtct gccgggggtc ttcctaccct ggcgcggtac
     1261 cggcaaccgg cgcaccgtga cgacgtggct tctgtcgatc gcgtccggga tggcaccgaa
     1321 cgtctggtgg tcgacacggt caccgatctt gtcgccgagc tgcacccgca ccgtgtccca
     1381 ccggtgcatc tggacagcag cctggagcgc gatctgggct tcgacagcct cgccctggcc
     1441 gagttggtca cgcgcctcga ggaagttgcg ggcatggcgc tgccggcgag cctgctgcgc
     1501 gtggtggaca cccctcgtga tctcgcgcgc gctgtccgtc ccgccgcgcg cgaccttcct
     1561 gcggcgttcg gctcggctct tcccgccgca ggggcacgaa ccccacagga ggtgtcgacg
     1621 ctgggtgcca gcctcgactg gcacacgtcc actcacccgg atcgggtgca cgtccggctg
     1681 ctcggcgagg acggcgctgt caccgacctc acgtacgggc agcttcgtca ccaggctcgc
     1741 gcgctggcgg ccgggctcct cgacgccggc gtcgcgcccg gggagtccgt cgcgttgatg
     1801 ctgccgaccg ggaccgacta cttcgtgtcg ttcatggggg tggtcctcgc cggggcagtc
     1861 ccggtcccca tctacccgcc ggcgcggccc gcccagctgg aggaacacct cgaacgccag
     1921 agccggatcc tcgacaacgc tcgggccgcg ctgttgatca ccgtgcccga ggcccggcgg
     1981 gtgtctcgcc tcctgcgatc aggagtcgag tcgctgcgcg gcatccgcac cccgggcgaa
     2041 ctgctcaccg ctcgccagac gacgctgccg gtgcccgggc ccgacgacat cgcgctgttg
     2101 cagtacacct cgggcagcac cggcagtccc aagggggtcg tgctcaccca ccggcagctg
     2161 ctggccaaca tcacggcgat ggcccgcgcg gcccgggtca cccccgacga cgtgttcgtc
     2221 agctggctgc ccctctacca cgacatgggc ctgatcggcg cgtggctcgg cagcctccac
     2281 gtcggcatgc ccctcgtgtc gatgtcgccg ctgtcgttcc tggtccggcc cgtccggtgg
     2341 ttgcaggtca tcgacgagca ccgggcaacg atctccgcag caccgaactt cgccttcgag
     2401 ctgtgcctcc gcaaggcgga cgacaccgag ctccgagcac tcgatctgag ctgctggcgt
     2461 ctcgccttca acggcgccga agccgtgcac cccaccacga tccgtcgctt caccgcgcgt
     2521 ctcgcgccga gcggcctccg cccggagacg ctcatgcccg tctacggcct ggccgaagcc
     2581 gccgtcgggc tcgcattccc acccgtgggc aggccaccac tgatcgaccg gatcgaccgc
     2641 agcgagttcg tcgcctccgg ccgcgcccga cccatcgacg acgatcagct ccccgtcgac
     2701 gtcgtcgact tcgtggcctg tggtcgcccc ctgcccggct acgagctgcg cgtcgtcgac
     2761 cacaccgacc gggagctccc ggaacgtcac gagggtcacc tccacttccg cgggccctca
     2821 gccacgacgg gctacttccg caacgccgcc gccacccgcg ccatgttcca cgacgactgg
     2881 ctcgacacgg gcgatctcgg gtacctcgcc gacggcgacc tctacgtcac agggcgggtc
     2941 aaggacctca tcatccgcgc cggtcgcaac ctccaccccg acgagctcga acagaccatc
     3001 ggacgcatcc ttggcatccg ccggggatgc gtcgccgtct tcgccgcgcc cgatcccacc
     3061 gcagccaccg aacgcctcgt cgtcgtcgcc gagacccgcg agaccgacgc ggagaccctg
     3121 accacgatcc gcgaccgggt cgccgccgcc accatcgctc tcgtcgagac cccacccgac
     3181 gacgtcgtgc tcgccccacc cggcaccgtg cccaagacct ccagcggcaa ggtccgccgc
     3241 gccgagaccc gcgcccgcta cgagtccggc accctcaacc cccaggcgcg ggcggtgtgg
     3301 cgccaggcct cccgctacgc cctcggacgc ctccccgtgc gtggccgacg catccgcagg
     3361 tcggtcggcg acgcgctcca caccgcactc ggatgggcag tcctcgccgc catcgggctc
     3421 ccgctgggcg cgctgctcac cgtcctgccc ggcgcccggc tccgctggac cctcaccacg
     3481 tgggcagctc gaagcgccct acgactgacc ggcacgacgg tgcacgtcca cggcgagctg
     3541 ccgccacccg gtcccgccgt catcgtcgcc aaccacgcca gctggatcga cggcctggtc
     3601 ctcacggccg tcatccccgg ccggatccgg ttcgtggccg gcgaggtctt cgctgatcag
     3661 gcgctcaccg gctcggtcct gcgccgcatc gacactgtgt tcgtcgaacg atgggaccga
     3721 gaacaaggcg tcgccgacgc cgcccacctc gccgagctga cccacgccgg cgcccggctc
     3781 gccgtgttcc ctgaaggcgg cctgtcaccc ctccccggtc tgcgaccgtt ccacctcggc
     3841 ggcttcgccg ctgccgccgc cggcgctgtc cccgtcatcc cggtcgccgt gcgcggcagc
     3901 cgctgggtcc tccgccccgg ccagcgccac ctgcgccgtg gagccatcga cgtcttcatc
     3961 ggcgacccca tcgaaccggt cggcgacgac tggcacgccg ccatcgagct ccgcgacgcc
     4021 gcccgcgccc acatcctgcg ccacggcggc gaacccgacc tcgcgtgacg cacagccggg
     4081 ggactagccg gcatcggcgc cgagcccgtc gcctggcacc gtgggtggga tcctgcgccc
     4141 gctgacccgc tcaggctcga gacgcacgaa gtggtctcgg tcaccaggcg cccacggatg
     4201 gacgccgaca gcgcggaggc gctcgcgctc ggcgggctct gtgacctcat ggagcgtgcc
     4261 gtggaccatg acgctcgtcc cgctgtggga gttctcgtcg atctcgtcga cctcgatcac
     4321 ggcgtgggcg ccgagtgaag ctgcgaccag cttggtgccg ggcgtggtgc ggaagatgac
     4381 cgaacggcgg tggatcacgt agttcaccgg gtacacgagc ggacgccggt ggaactcgat
     4441 cactccgacg cgaccgagat gacgatgttc cacgaagcgc cagcactcgt cttcggtcag
     4501 gtgcgcaagg ccatcaactg ccatctccat cacctctctg cacgacaacc tgctcaacgt
     4561 ccatcgtggg gcccgtgcga gcgctgcggt agggcccaat gtccgtcgct ggcgctgcgg
     4621 cgggaccatc ggccctgctg ttgcgcaccg gcgctgcgta acgtccgcgg cacacggcga
     4681 tcgaggaggt gccgacgctg tcgacggtca ccccagggcc acactcgtcg acgatcgggc
     4741 tgacgtccac agccgcggcc gcccgcctcg cagtcgacgg tgccaacaag ctccccccgc
     4801 cggtcgagcg ctcggcgctc cggcgcctcg tcgcccagtt cgtccacttc ttcgcgctga
     4861 tgctgtgggt ggcggccgcg ctcgcgttcg tggccggcat ccccgagctc ggtgcagcca
     4921 tcgccctcgt gatcgtgctc aacgcgctgt ttgccttctt ccaggagagc cgcgccggac
     4981 aggcagccgc ccgcctccgg gccctgctgc ccatgcaggt caccgtcgtg cgcgatggtc
     5041 gccggcagca ggtcgacagc acgcaggtgg tcgtcggcga cgtgttggct ctcgaaggcg
     5101 gggatcgcat ccctgccgac gggacggtcc tcgtcgcgca cgggctgttg gtcgacacgg
     5161 ctctgctgac cggcgagagc gaacccgagc ggatggcaga gggcgacgac atctcggccg
     5221 ggacgttcgt cgtcgaaggc gaggcgctcg ctttggtgtc cgcgaccggc ggggggacgc
     5281 gactggccga catcgccgcc atgacgacgg cgacggattc gccagcgagt cccctcacca
     5341 tcgcgctgca ccgagtcgtc cgcaccgtgg cgctcatcgc ggtgtcggtc ggcgccagct
     5401 tcttcatcat catgctggcg gtggaccgcc cggcctcgga cgggttggtg ctcgcgatcg
     5461 gggtgaccgt ggccctcgtg cccgaggcgc tgctgcccac cgtgacgctc tccctcgcgt
     5521 ggggggcgga gcagatggct caccgccgcg tcctcgtccg gaacctcgag gccgtggaga
     5581 cgctcgggtc caccacgttc atctgcaccg acaagaccgg cacgctcacc cacaaccaga
     5641 tgacggtcgt cgacgtctgg acgcccacgg ccacggcgac gatcgaggag cccggctaca
     5701 gcccggactc ccccgttctg gtcgcgggtg atcgcagcgc gctggaggcg ctcgcgcgtg
     5761 ccgccgtccg gtgttccacc gggtacgtgc atcgcgtgca gggtgcgtgg cagccccacg
     5821 gggacccgat ggaggtggcc atcgacgtgt tcgcccatcg tctcggactg gacaccgaag
     5881 gtgaccgtgc tctggacgtg gctcgtgcca ggttcccctt cgatccgcgc cgacgacgga
     5941 tgtcggtcgt cgtcggagac gagctcttgg tgaagggagc accggacgca gtgctgcccc
     6001 gttgtgacga caccgagggc gctgccatcg cgctcgatcg gatgatggca gcgggtctgc
     6061 gggtcctcgc cgtctcccgc cggcccgtcg gaccagcggt gccggtcagc gccgacgagg
     6121 cggagaccgg cctcgagctg ctcggtctgg tcgccatgga ggacccaccc aggctcgagg
     6181 tccgcgacgc cctggccgcc tgtcgccggg ccggcgtccg gatcgcgatg gtcaccggcg
     6241 accatcccgg acccgctcgc accatcgccg accaggtcgg cctccgccga cctgacgacc
     6301 cggtcgtcgt cggccatgca ctgcccgaag acgacgatgc cctcggcgcg ctgatcgacc
     6361 acgacggctg cgtcgtggcc cgggccacac cggaggacaa ggtgcgcatc gcccgggccc
     6421 tgcgggcgcg cggccacgtg gtggccatga ccggtgacgg cgtcaacgac ggcccgtccc
     6481 tccacgaggc cgccgacctc gtgctgctcg acgacgcctt cgccagcatc gtggccgggg
     6541 tcgagcaggg ccgcgccacc tacggcaaca tgcgccgctt cctcgcctac cacctcaccg
     6601 acaacgtcgc ggagctcacg ccgttcctgg tgtgggcgct gtcgggtggc aacttcccgc
     6661 tcgcactcgg ggtgatgcag atcctggccc tcgacctcgg caccgacacc ctctccgcgg
     6721 tcgcgctcgg cgcggagccg ccgggcaagc acctcctgga cggccccccg gtcaagggtc
     6781 gcctgatgaa ccacagcgtg gtccgtcgct ccttcggtct gttcggaccg ttggtcgctg
     6841 cgctgacgat ggcagcgttc ctggtgtccc tgttcgtcag cggatggcgg cccggcgagg
     6901 tcttccccac cggaccggcc ctg
//
LOCUS       DATISU010000414         4617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_82529, whole genome shotgun sequence.
ACCESSION   DATISU010000414 DATISU010000000
VERSION     DATISU010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82529"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..485)
                     /locus_tag="VLR27_16410"
     CDS             complement(99..485)
                     /locus_tag="VLR27_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05096.1"
                     /translation="MAATRPTPKSTSGATVRPAATRAEKKRTGDKGVATVVSELWTLT
                     IDYAKQEIKDPLTGLVSYVVWGIATMVLVGIGSILLAIGALRALQTQTGSTFTGSLSW
                     APYGIVLFGAVVVLGSVGALIMRGKK"
     gene            complement(570..1199)
                     /locus_tag="VLR27_16415"
     CDS             complement(570..1199)
                     /locus_tag="VLR27_16415"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HSP05097.1"
                     /translation="MPAIPMFPLGSVLFPSLVLPLHIFEPRYRALIHDVLDGDREFGV
                     CLIERGAEVGGEDVRTTIGTVAQVHEAEELPDGRWAVVAVGVRRIRVERWLPDDPYPR
                     AEVVDLPDPEPSASEVEVLPEVVARLRTALARAAELGDTAVPATVELSIDPAMASHQA
                     SALAPIATIDQHALLSAETVGLRLARLHDLLGDAIEVLDLRLAAEGPGS"
     gene            1283..3013
                     /locus_tag="VLR27_16420"
     CDS             1283..3013
                     /locus_tag="VLR27_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSP05098.1"
                     /translation="MFDLVIRGGSVVDGTGAEPTVGDVAVQGDRIVAVGRDLPEGARE
                     IDATGRIVTPGFVDVHTHYDGQATWDEELAPSTPHGVTTVVMGNCGVGFAPVQPGEEQ
                     FLVELMEGVEDIPGTALNEGMTWGWESFEDYLDVLADRRWTADVGAMIAHGALRTYVM
                     GDRGARNEPATADDVSRMAELVARAIEAGAMGFSTSRTIGHTSVSGDPVPGTFAAEDE
                     LFAIARAISEVGRGVFEVAPSGIVSDDHVSPESEIEWMCRLGAETGVPVTYLQLQYDW
                     APDSWRKHLDLVARAREQGVDITAQVAARPFGVLLGLGARHRFLTAPSFAPLVDLPLA
                     EKVERMRDPQLRATLIEESRQMMAVLRDAMPTAAQLLDTFARTYPLGDPVDYEQPYEH
                     SFSGIAEAQGRDPIEVYYDALLERDGQAISMMTLFGNSEGNGDALYEMISEPGTVIGL
                     ADGGAHCNMICDASTPTWTLTHWVRDRDGARLPLELAVKKMTADTADLFRLADRGRIA
                     EGLRADLNVIDLDGLTLREPHLVADLPAGGTRFLQEAEGYEHTIVGGVVTRTGDEFTG
                     TRPGRLLRST"
     gene            complement(3138..>4617)
                     /gene="dacB"
                     /locus_tag="VLR27_16425"
     CDS             complement(3138..>4617)
                     /gene="dacB"
                     /locus_tag="VLR27_16425"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HSP05099.1"
                     /translation="AVAPGAGARRHLVTDSADAARAGGWLHRILVVLPVLALGALIAA
                     SLVMVDRLGETPTAEPALGQPRPSVPLLSVRRDLAPLADEAADRRLRTRLGEFVAGLP
                     PDTCLSVDAGDVRFDHRIDDPQVPASSTKIVTGVAVLVALGGDFRYTTTVSTPTPPVE
                     GVVAGDLHLIGGGDPILAQGEYAGRYRRPQEFSDIRLLVDAIAAAGVVRVEGAIVGDE
                     SRYDTERYHPAWPPRFITQDQTGPLSALLVNDGFVEWPITGAGPVKPALDPAAHAAEV
                     LRLLLEERGITVAGGSGSGPAPAGAPVVAEHRSPPLEDIVGQMLSESDNATAELLLKE
                     LGLQVGGAGTFSAGAASVTRVLGEAGFDVEGTTILDGSGLSSGNSLDCELLMALLEHP
                     PTADAIHDGLAVAGESGTLAERWQDTALAGRIRAKTGTLNQVTALAGVAATEGAGEAT
                     FALVVNVPEPERIQLETIAAQQRLAELLVAHPDTPDLSAFAP"
BASE COUNT          651 a         1689 c         1672 g          605 t
ORIGIN      
        1 cgcgtaggac ttggccgact cggtcgtgtc gtcgacgtcc tccttcagct cacggagctt
       61 ggcctcgatg tcgtcgcggg tgatcttgtc ggtggccact acttcttccc tcgcatgatg
      121 agggcgccga ccgagccgag cacgaccacg gcgccgaaca ggacgatgcc gtagggggcc
      181 cacgacaggc tgcccgtgaa cgtggagccg gtctgggtct gcagggctcg cagcgcgccg
      241 atggccagca ggatggaacc gatgccgacc agcaccatcg tggcgatgcc ccacacgacg
      301 taggagacca ggcccgtgag cgggtccttg atctcctgct tcgcgtagtc gatcgtcagc
      361 gtccacagct cggacacgac cgtggcgacg cccttgtcgc cggtgcgctt cttctcggcg
      421 cgggtggccg ccggtcggac ggtcgcgccg gacgtcgact tcggtgtggg tctggttgcg
      481 gccacgctgc tcctcggtcg gctgcccggc gttctacccg gtccggtcgg ttccgggcaa
      541 ccaccacccc ctacccggtc acgcgccggt caggacccgg ggccctccgc agccagccgc
      601 aggtcgagca cctcgatcgc gtcgccgagc aggtcgtgga gccgggcgag gcggaggccc
      661 acggtctcgg ccgacagcag cgcgtgctgg tcgatggtgg cgatcggtgc cagggcgctg
      721 gcctggtggc tggccatcgc cggatcgatc gacagctcga cggtggccgg gacggcggtg
      781 tcgccgagct cggcggcgcg agccagggcg gtgcgcagcc gggccaccac ctcgggcagc
      841 acctcgacct ccgaggccga cggctccggg tcgggcaggt cgacgacctc ggcccggggg
      901 taggggtcgt ccgggagcca ccgctcgacc cggatgcggc gcacgccgac cgcgaccacg
      961 gcccagcgcc cgtccgggag ctcctcggcc tcgtggacct gggcgacggt gccgatcgtg
     1021 gtgcgcacgt cctccccgcc gacctcggcc cctcgctcga tcaggcagac cccgaactcg
     1081 cggtcgccgt cgagcacgtc gtggatcagg gcccggtagc gcggttcgaa gatgtgcagt
     1141 ggcagcacca ggctcgggaa cagcacggag ccgaggggga acatgggaat cgccggcaca
     1201 cggcgacact acgaccgagg tcccgggtgc gtgccgcgac ctacggtgtc ggcaacagct
     1261 gtttccaagg gggggcacca ccatgttcga tctcgtgatc cgcggcggct cggtcgtcga
     1321 cggcaccggc gccgagccca cggtcggcga cgtcgccgtg cagggcgacc gcatcgtcgc
     1381 cgtcggccgc gaccttcccg agggtgcccg ggagatcgac gccaccggac gcatcgtcac
     1441 gccggggttc gtcgacgtcc acacccacta cgacggccag gccacctggg acgaggagct
     1501 ggcaccgtcc acgccgcacg gcgtcaccac cgtggtgatg ggcaactgcg gcgtcggctt
     1561 cgcgccggtg cagcccggcg aggagcagtt cctcgtcgag ctcatggagg gcgtggagga
     1621 catcccgggc accgccctca acgaggggat gacctggggg tgggagagct tcgaggacta
     1681 cctcgacgtc ctcgccgatc gccgctggac ggccgacgtc ggggccatga tcgcccacgg
     1741 cgcgctgcgc acctacgtga tgggcgaccg cggcgcccgc aacgagcccg ccaccgcaga
     1801 cgacgtgtcc cgcatggccg agctggtcgc ccgcgccatc gaggccggtg ccatgggctt
     1861 ctccacgtcg cgcacgatcg ggcacacgtc ggtctccggc gaccccgtgc ccggcacctt
     1921 cgccgccgag gacgagctgt tcgccatcgc ccgtgccatc agcgaggtgg ggcggggcgt
     1981 gttcgaggtc gccccctccg gcatcgtcag cgacgaccac gtgtcccccg agtccgagat
     2041 cgagtggatg tgccggctcg gggccgagac cggcgtcccg gtgacctacc tgcagctgca
     2101 gtacgactgg gcgcccgaca gctggcgcaa gcacctcgac ctggtggccc gagcccgcga
     2161 gcagggcgtc gacatcaccg cccaggtcgc cgcccgaccc ttcggcgtgc tgctcggcct
     2221 cggtgcccgg caccgcttcc tcaccgcgcc gagcttcgcc ccgctcgtcg acctgcccct
     2281 ggccgagaag gtcgagcgca tgcgcgaccc gcagctgcgg gccacgctca tcgaggagag
     2341 ccgccagatg atggccgtcc tccgggacgc catgcccacg gccgcccagc tgctcgacac
     2401 cttcgcccgc acctacccgc tcggcgatcc ggtcgactac gagcagccct acgagcactc
     2461 gttctccggc atcgccgagg cccagggccg cgatcccatc gaggtgtact acgacgccct
     2521 cctcgagcgc gacgggcagg ccatctcgat gatgacgctg ttcggcaaca gcgagggcaa
     2581 cggcgacgcc ctgtacgaga tgatcagcga gccgggcacg gtcatcggcc tggccgacgg
     2641 cggcgcccac tgcaacatga tctgcgacgc ctcgacgccg acctggaccc tcacccactg
     2701 ggtccgcgac cgagacggcg cccgcctgcc actcgagctc gcggtcaaga agatgaccgc
     2761 cgacaccgcc gacctgttcc gcctggccga ccggggtcgc atcgccgagg gcctgcgcgc
     2821 cgacctcaac gtcatcgacc tcgacgggct gacgctgcgc gagccgcacc tcgtcgccga
     2881 cctgcccgcc ggcggcaccc gcttcctcca ggaggccgaa ggctacgagc acaccatcgt
     2941 cggcggggtg gtcacccgca ccggcgacga gttcaccggt actcgtcccg gacgcctcct
     3001 gcggagcacc tgagccgagc cgacaggcga ggcgacccag gagcacggcg tcattcggcg
     3061 gcgaagccgc caaccgagtc gcgagaggac gtgacgcatc ggcgcaggtc ccaaaaccct
     3121 tctggcagca gctgcggcta cggcgcgaag gccgacaggt ccggcgtgtc ggggtgcgcc
     3181 accagcagct cggccaggcg ctgctgggcg gcgatcgtct cgagctggat gcgctccggc
     3241 tcgggcacgt tgacgaccag cgcgaaggtc gcctccccgg cgccctcggt cgcggccacg
     3301 ccggccaggg ccgtgacctg gttgagggtg ccggtcttgg cccggatgcg tccggccagg
     3361 gcggtgtcct gccagcgctc ggcgagcgtg ccggactccc cggccaccgc caagccgtcg
     3421 tggatggcgt cggctgtcgg tgggtgctcc agcagagcca tgagcagctc gcagtcgagc
     3481 gagttgcccg acgagagccc ggaaccgtcg aggatggtgg tgccctccac gtcgaagccg
     3541 gcctcgccca gcacccgcgt caccgacgcc gcaccggcgg agaacgtgcc ggcgccgccg
     3601 acctgcaggc cgagctcctt gaggagcagc tcggcggtgg cgttgtcgga ctccgacagc
     3661 atctggccca cgatgtcctc cagcggcggc gagcggtgct cggcgacgac gggcgccccg
     3721 gccggggccg gacccgagcc cgagccgccg gcgacggtga tgccccgctc ctcgagcagc
     3781 agtcggagga cctcggcggc gtgggcggcg ggatccagcg ccggcttcac cgggccggca
     3841 ccggtgatcg gccactccac gaagccgtcg ttcaccagga gggcggagag cggaccggtc
     3901 tggtcctggg tgatgaagcg gggcggccag gccgggtggt agcgctcggt gtcgtagcgg
     3961 gactcgtcgc cgacgatggc gccctcgacc cgcaccacgc cggcggcggc gatggcgtcg
     4021 acgagcagcc ggatgtcgga gaactcctgg gggcggcggt accggccggc gtactccccc
     4081 tgggcgagga tcgggtcgcc gccgccgatc aggtggaggt ccccggccac cacgccctcc
     4141 accggtggcg tcggcgtgga cacggtggtc gtgtaccgga agtcgccgcc gagggcgacg
     4201 agcacggcga cgccggtgac gatcttcgtg gacgacgccg gcacctgcgg gtcgtcgatg
     4261 cggtggtcga agcggacgtc accggcatcc acgctgaggc aggtgtccgg cggcagtccg
     4321 gcgacgaact cgcccagccg ggtgcgcagg cgacggtcgg cggcctcgtc ggcgaggggc
     4381 gcgaggtcac gtcgcaccga cagcaggggc acgctgggcc gcggctgtcc gagcgcgggt
     4441 tcggcggtgg gggtctcgcc gagtcgatcg accatcacca gcgacgcggc gatgagcgcg
     4501 ccgagcgcga ggacgggcag caccaccagg atccggtgga gccagccccc cgcccgggct
     4561 gcgtcagcgc tgtcggtcac gaggtgccgt cgagcccccg cgcccggcgc cactgct
//
LOCUS       DATISU010000415         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_921605, whole genome shotgun sequence.
ACCESSION   DATISU010000415 DATISU010000000
VERSION     DATISU010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921605"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VLR27_16430"
     CDS             <1..552
                     /locus_tag="VLR27_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP05100.1"
                     /translation="EHANRFPHEFSGGQRQRIGIARALALEPKVLVLDEPVSALDVSV
                     QAGVINLLEDLQNRLGLAYIFIAHDLSVVRHISDRVAVMYLGQVMETADAGDLYSSPA
                     HPYTQALLSAVPVPDPRRERARTRIVLEGDVPSPVNPPSGCRFRTRCWKATEKCAEEV
                     PALIDRGQGHPVACHYAEVQVVI"
     gene            557..>2174
                     /locus_tag="VLR27_16435"
     CDS             557..>2174
                     /locus_tag="VLR27_16435"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSP05101.1"
                     /translation="MGRSTRPAPWWVALLVVLAVVAAGCSTFSDDEDTGAVEAPEGTL
                     PRAATLMRVAAGDWPDCLNPVTCTDPTSRALVLQHVLPRLMEVDADGRYVPSPVLAGG
                     PEVRVDEDTGEQTITFVLAEEARWHDGRPITSSDVRGTWLAHTSTPGSTARGHDLITA
                     VDDTDPLVARGTLRRPWTDWPELFGGYTGWLLQADAFGSGTDLTDRFDDFLPFGAGPY
                     ELVSFDDQSLVLVARSEHWDPERQAGIDQVRIDHFVDLGTDGAVGRVSVPGSIDLLIP
                     AAEMPEAPSRFDLRRSPTPSVVGLFFDRRTPTLASPGMRTAIDAALDRRDLVDLVGVD
                     ADDLVTCLGWLPGEETCGEDLEEEGVSTDAVDLSLFLDGWALLEDGTRVRDGQPLTVP
                     VSYDPLLEGADEIADAVAEALVAEGFAAETQAVSAGTWARRDRESGTGIGVFAARLGT
                     AERVAALYRCDEGSRNPMGWCEPEPQALATELISAPSERERQTVAADLGELAATSLAW
                     LPLHQRTTRWLVDPERIAVPDRAPLGSGPLG"
BASE COUNT          268 a          862 c          751 g          293 t
ORIGIN      
        1 gagcacgcca accggttccc gcacgagttc tccggcggcc agcgccagcg catcggcatc
       61 gcccgggccc tcgccctcga gcccaaggtg ctggtgctcg acgagccggt ctccgccctc
      121 gacgtgtcgg tccaggccgg cgtcatcaac ctgctcgagg acctccagaa ccgcctcggc
      181 ctcgcctaca tcttcatcgc ccacgacctg tccgtcgtgc gccacatctc ggaccgggtc
      241 gccgtgatgt acctcggcca ggtcatggag accgccgacg ccggcgacct gtacagctcg
      301 ccggcgcacc cgtacacgca ggcgctgctg tcggccgtgc cggtgcccga cccccgccgc
      361 gagcgggccc gcacccgcat cgtgctcgag ggcgacgtgc ccagcccggt gaacccgccc
      421 tcggggtgcc ggttccgcac gcgctgctgg aaggccaccg agaagtgcgc cgaggaggtg
      481 ccggcgctga tcgaccgggg ccagggacat cccgtggcgt gccactacgc cgaggtccag
      541 gtcgtcatct agcccgatgg gtcgctccac ccgcccagcc ccgtggtggg tcgccctgct
      601 cgtggtcctc gccgtggtgg cggccggctg ctccaccttc tccgacgacg aggacacggg
      661 tgccgtcgag gcacccgagg gcacgctgcc ccgggccgcc acgctgatgc gcgtcgccgc
      721 cggcgactgg cccgactgcc tgaacccggt cacctgcacc gatcccacct ctcgggccct
      781 ggtgctgcag cacgtcctgc ccaggctgat ggaggtcgac gccgacggcc gctacgtgcc
      841 gtcgccggtg ctggccggtg gccccgaggt ccgggtcgat gaggacacgg gggagcagac
      901 gatcaccttc gtgctggccg aggaggcccg ctggcacgac ggccgcccga tcacgagcag
      961 cgacgtccgc gggacctggc tggcccacac ctccacgccc ggatcgaccg ctcgtggcca
     1021 cgacctgatc acggcggtgg acgacacgga cccgctggtc gcccggggca cgctgcgccg
     1081 gccgtggacc gactggcccg agctgttcgg cggctacacc ggctggctgc tccaggccga
     1141 tgccttcggc agcggcaccg acctgaccga ccgcttcgac gacttcctgc ccttcggcgc
     1201 cggcccctac gagctggtgt ccttcgacga ccagtccctg gtgctggtcg ctcgctccga
     1261 gcactgggac cccgagcgcc aggccgggat cgaccaggtg cgcatcgacc acttcgtcga
     1321 cctcggcacc gacggcgccg tcggtcgggt gagcgtcccc ggcagcatcg acctgctgat
     1381 ccctgccgcc gagatgcccg aggccccgtc ccgcttcgac ctgcggcgct cgccgacgcc
     1441 gtcggtggtg ggcctgttct tcgaccgccg caccccgacg ctggcctcac cgggcatgcg
     1501 gaccgcgatc gacgcggcgc tggaccgccg ggacctggtc gacctcgtgg gggtggacgc
     1561 cgacgacctg gtcacctgcc tcggctggct gcccggcgag gagacgtgcg gtgaggacct
     1621 cgaggaggag ggcgtgtcga ccgacgccgt cgacctctcg ctcttcctgg acggttgggc
     1681 gctgctggag gacggcaccc gggtccgcga cggccagccc ctcaccgtcc ccgtctccta
     1741 cgaccccctg ctggaggggg ccgacgagat cgccgacgcc gtcgccgagg cgctcgtcgc
     1801 cgagggcttc gccgccgaga cccaggcggt gtcggccggg acctgggccc gccgcgaccg
     1861 cgagagcggg accggcatcg gcgtgttcgc cgcccgcctc ggcaccgccg agcgtgtggc
     1921 tgcgctgtac cgctgcgacg agggctcccg gaacccgatg ggctggtgcg agccggaacc
     1981 ccaggcgctg gccaccgagc tgatcagcgc cccgagcgag cgggagcggc agaccgtcgc
     2041 cgcagacctg ggcgagctgg ccgccacgtc gctggcctgg ctgcccctcc accagcgcac
     2101 cacccgctgg ctggtcgacc ccgagcgcat cgccgtcccc gaccgggccc ccctcggctc
     2161 cggccccctc ggcg
//
LOCUS       DATISU010000416        11807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_780359, whole genome shotgun sequence.
ACCESSION   DATISU010000416 DATISU010000000
VERSION     DATISU010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 11807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11807
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780359"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VLR27_16440"
     CDS             complement(<1..554)
                     /locus_tag="VLR27_16440"
                     /inference="COORDINATES: protein motif:HMM:NF026010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HSP05102.1"
                     /translation="MAEADDDRGAAQSHTLEHLLERWWEQKEARLSPTTAREYERIIA
                     KRLVPTMGTRKVASIAPADLDTYYMRLAKEGLAPASIRQVHAVLSGALGQAVKWRWIT
                     MNPARDATLPKASRTPIDPPTPEQARRLLALADEHSAEIGMFVRLSAAMGCRRAEVCG
                     LRWIDIDQSRRTVKIRRSVVDVAGR"
     gene            complement(814..887)
                     /locus_tag="VLR27_16445"
     tRNA            complement(814..887)
                     /locus_tag="VLR27_16445"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(851..853),aa:Lys,seq:ctt)
     gene            complement(948..1025)
                     /locus_tag="VLR27_16450"
     tRNA            complement(948..1025)
                     /locus_tag="VLR27_16450"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(988..990),aa:His,seq:gtg)
     gene            1107..1180
                     /locus_tag="VLR27_16455"
     tRNA            1107..1180
                     /locus_tag="VLR27_16455"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1141..1143,aa:Arg,seq:tct)
     gene            complement(1207..1770)
                     /locus_tag="VLR27_16460"
     CDS             complement(1207..1770)
                     /locus_tag="VLR27_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05103.1"
                     /translation="MKPLPKLLLAAALLLGAVGLVVALVQPGGLADDDGDTETAQPEL
                     TETETETETQTEDEAGTETDTETDTDTQTEDESETETDTDTATETETTSTPASDGTSS
                     TSTTEATGTAPTTTAAVGSAGATTTTTSRPGGTTGSGGGSGLDSDSGRTVPRDLEDGL
                     ADTGGTSAIGAAVVIGLAGLALRRRTT"
     gene            complement(1847..1920)
                     /locus_tag="VLR27_16465"
     tRNA            complement(1847..1920)
                     /locus_tag="VLR27_16465"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1886..1888),aa:Gly,seq:tcc)
     gene            complement(1928..3391)
                     /gene="mftF"
                     /locus_tag="VLR27_16470"
     CDS             complement(1928..3391)
                     /gene="mftF"
                     /locus_tag="VLR27_16470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03965.1"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="HSP05104.1"
                     /translation="MAEGFPEHIALALDAETRRSADGRSLLGGAPMRLLRFSDAGAAA
                     VDRVLAGGAVPTTGPEALVARRLLDAGMVHPRPARTPGLTLSVVVPVRDHAAELASLL
                     DALHVPAGTEVVVVDDGTPDPAAVPGAVAGRAEVLRHDSSLGPGAARDTGWRASSGDV
                     VAFVDADVHLGDGWLDPLLAHLADPAVGLVAPRVRSRPGPSLLERYERHRSPLDLGAR
                     EARVVPRGRVSYVPTATVVCRRELLEQLDGFEPDMRVGEDVDLVWRAVEAGWTVRYEP
                     AAEVEHRPRGSWGALVRQRMAYGSAAAPLDQRHPGAVAPVELNAWSLLAWVLAVIGGR
                     RGALAGAGVAVGSSLALVPKLRGRVDEPVRSSLELAGGGTLRAGAWLGRSVWRAWLPL
                     ALVGSLRSRRVRRATLAAAVVPALLDRRAAGPQPGVDPVRWTLIHALDQAAYCAGVWR
                     GAWRCRSGRALLPRLSGIPGVTRDRGNRDRSGARLAS"
     gene            3455..3586
                     /gene="mftA"
                     /locus_tag="VLR27_16475"
     CDS             3455..3586
                     /gene="mftA"
                     /locus_tag="VLR27_16475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03969.1"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes
                     in Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin precursor MftA"
                     /protein_id="HSP05105.1"
                     /translation="MTVTEPTTESSAEAEDQEQATEELVTDDVLVEEISIDGMCGVY"
     gene            3605..3889
                     /gene="mftB"
                     /locus_tag="VLR27_16480"
     CDS             3605..3889
                     /gene="mftB"
                     /locus_tag="VLR27_16480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03967.2"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ,
                     a cofactor that derives entirely from a Tyr and a Glu in
                     the precursor PqqA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="HSP05106.1"
                     /translation="MAFDPADRYRLSPRVGLRPEPFGALAYHYDTRRLVFLKSNDLVA
                     LVRSLEQHDSAAAAIDALVDPTTGRRSAYVTALASLCESGVVEPLLEPAA"
     gene            3886..5040
                     /gene="mftC"
                     /locus_tag="VLR27_16485"
     CDS             3886..5040
                     /gene="mftC"
                     /locus_tag="VLR27_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443074.1"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron
                     carrier mycofactocin from the terminal Val-Tyr dipeptide
                     of the precursor peptide MftA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="HSP05107.1"
                     /translation="MSTIPKTPPLVEQLKGGLDAPICLTWELTYACNLACVHCLSSSG
                     RRDPKELSTDEAKDLIDQLAALQVFYVNIGGGEPMLRTDFFEIVEHSVTNGVGVKFST
                     NGTYLDRAAAERLVELPYLDVQISLDGMTPEVNDAVRGEGSHAKAIEAMGHLRDVGFE
                     DFKISIVCTRHNVDQLDEFKALADDHGAQLRITRLRPSGRGVDSYHDLRLTNPQQRQV
                     FHWILDHPDVLTGDSFFHLNALTTETGGPNLPGLNMCGAGRVVCLIDPIGDVYACPFV
                     IHDEFLAGNVRDAGGFAHVWQESSLFTELREPTSAGACASCGSYDACQGGCMATKFFT
                     GLPLDGPDPDCIHGHADEALAGIAAGSAPRPSQDHSRPGAAVPVALTRKS"
     gene            complement(5094..6293)
                     /gene="mftD"
                     /locus_tag="VLR27_16490"
     CDS             complement(5094..6293)
                     /gene="mftD"
                     /locus_tag="VLR27_16490"
                     /EC_number="1.4.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443197.1"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the
                     non-exchangeable NADH of TIGR03971 family SDR-type
                     oxidoreductases; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="HSP05108.1"
                     /translation="MAHKWFESVEIARQRAEKILPRSVFGALKAGSEAGHTLADNTKA
                     FSELGFVPTVADKPAERDQSTQVMGQSISMPVLISPTGVQMVHPEAEIGIARASANRG
                     TALGLSNFGSTPVETVMAENPQTFFQIYWCGTREQMTSRMDRAKRAGVAGLIVTLDWS
                     FSHGRDWGSPDIPEVLDLKTSIKHSPELLTSPRYAWRYGKYTLENRSLPDLHVPNMVP
                     EGEAPPGFFEAYGQWMGTPPPSWDDLRWLREQWDGPFMLKGIMRVDDAKRAVDAGYSA
                     ISVSNHGGNNLDTTPAPIRALPLIAEAVGDQIEVLMDGGIRRGSDVVKAVALGAKAVM
                     IGRASLWALAVGGQPGVENVLDVLRNGVDSAMLGLGKRTLADLGPLDIVVPDDFSDSV
                     RQAEATE"
     gene            6331..7035
                     /gene="mftE"
                     /locus_tag="VLR27_16495"
     CDS             6331..7035
                     /gene="mftE"
                     /locus_tag="VLR27_16495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="HSP05109.1"
                     /translation="MTAPYPEKRQLGSQTWPEVAETPPRVLLVPMGSCEQHGPHLPLD
                     TDTRIAVAVAERVAAGRDGVVVAPAVAYGSSGEHQGFAGTLSIGTAALRSVLIELGRS
                     AFPAADPGAFASLVFVNGHGGNHEALRDAVDVLVGEGRSVSAWWPSIAGGDAHAGRTE
                     TSLLLAIAGDVVGSARPVGATEPLAALLEPMRAGGVGAVAPNGVLGDATDASAEEGEA
                     LLTALVDDLAGRLSVS"
     gene            7131..8285
                     /locus_tag="VLR27_16500"
     CDS             7131..8285
                     /locus_tag="VLR27_16500"
                     /EC_number="1.1.99.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HSP05110.1"
                     /translation="MKTRAAILWEKNQDWSVEEIDLDPPQTGEVLIKLVGSGLCHSDE
                     HLRTGDMVVPQETADALGLKQFPIIGGHEGAGEVVEVGPNVTGLEVGDHVVLGFIPAC
                     GKCPSCSRGRSNLCDYGAFLLAGRQIADFTARHHAADGTDLGLMCLLGTFGEYTVVNE
                     ASCIKIEKDIPLDKAALVGCGVTTGWGSATYAADVQPGETVVVVGAGGIGMNAIQGAA
                     MNGARHIVAVDPVEFKREQAMVFGATHTAASIEEATELVGQITWGQNAEKAIITVGVG
                     DGDLIAPVMGLTAKGGRVVHTSVAPMERNDVALNLFDLTLQQKQLVGSIFGSANPRYD
                     VPRLLRLYQEGKLKLDELITREYDLADINKGYDDMNDGNNVRGFVRYNQS"
     gene            8286..9230
                     /locus_tag="VLR27_16505"
     CDS             8286..9230
                     /locus_tag="VLR27_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSP05111.1"
                     /translation="MTRSDHGFDRSLIESAAVEVVGRRRELELVTAALAASRHVLLEG
                     PPGTGKSTLLRAIAERAGLELVFVEGNAELTPARLAGHFDPARVLTDGYAEDVFVDGP
                     LLRALRDGGLLYVEEINRVPEETLNLLITVMSEGELTVPRLGRVVAAPGFRMVAAMNP
                     YDAVGTARISGAVYDRVCRVSMGYQSLDDELAIVVHEAKTAGATDAAGDDEFVRRVVS
                     VVRGTREHPDVRIGSSVRGAIDMVLIADELAGLRETDASEPDTALDAACMALSGRIRV
                     HESCHRTPEDIVAELWDLYATTVDLGEGGGDGEDPGKG"
     gene            9368..10192
                     /locus_tag="VLR27_16510"
     CDS             9368..10192
                     /locus_tag="VLR27_16510"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HSP05112.1"
                     /translation="MSPELGVLDESAIEESLADRPDETLAMLADMTGATDERLRALAR
                     RLAGRIVIGMARADAARRRGIGKMRSLPMDDTGGDIDMDASIEALQLASATGTAPRAD
                     ELTVRTWARPAVAICLVVDRSGSMSGDRLAASAIAAAAVAERAPDDFSVLAFSDKVMI
                     IKGQSQRRSVDDVVEDVFRLRGHGPTDLGLALRVAADQLASSGAQRKRVVLLSDCRPT
                     KGTEPERDAGAIDELHVIAPAEDAADAEAFARAVGARFAALSGPSQIPSVFAQLAD"
     gene            10229..10996
                     /gene="heR"
                     /locus_tag="VLR27_16515"
     CDS             10229..10996
                     /gene="heR"
                     /locus_tag="VLR27_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HSP05113.1"
                     /translation="MAATDTGMTDERLADLRRWNLGLTVLHVAQAVAVLVLASDFAIT
                     VTSSFPTGPPGAAPPAPEALFDIPIGAAVAVFLALAALDHLVTATIGRRTYEADLRRG
                     INRFRWVEYSFSATLMIVLIASYSGITEITAVIGIAGANVAMILFGWLQERMNPPGRS
                     STSMLAFWFGCVAGVAPWIAIAVNIFGADTVPGFVYGIFVAQLVFFSSFGLNQWLQYR
                     GIGPWSDYAFGEKAYLVLSLGAKSVLAWQIFGGSLAG"
     gene            11034..11573
                     /locus_tag="VLR27_16520"
     CDS             11034..11573
                     /locus_tag="VLR27_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HSP05114.1"
                     /translation="MADELNGKTIAFLVAQEGIEQVELTDPWKKVEESGGQPILVAPE
                     AGTVQAFDHLDAADTFDVDRTTADLRVDDIDGVVLPGGVANPDQLRTDEAAVSFLRDF
                     VQSGKPVAVICHGPWTLVEAGVLEGRTLTSWPSLQTDIRNAGGTWVDQEVCRDGNLVS
                     SRNPGDLPAFTDAMVELFS"
     gene            complement(11582..11658)
                     /locus_tag="VLR27_16525"
     tRNA            complement(11582..11658)
                     /locus_tag="VLR27_16525"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11622..11624),aa:Pro,seq:tgg)
BASE COUNT         1648 a         4363 c         4148 g         1648 t
ORIGIN      
        1 cggccggcga cgtcgacgac ggaccgacgg atcttcaccg tgcgccggga ctgatcgatg
       61 tcgatccatc gcagtccgca cacctcggcg cgccggcaac ccatcgccgc agacaggcga
      121 acgaacatcc cgatctctgc cgagtgctcg tcagcgagcg cgagcagccg gcgcgcctgt
      181 tctggcgtcg gcggatcgat cggtgtgcgt gaggcctttg gcagggtcgc gtcccgggcc
      241 gggttcatcg tgatccagcg ccacttcacc gcctgaccca gcgcgccgct cagcacggcg
      301 tgcacttggc ggatgctggc gggggcgagg ccctccttcg ccagccgcat gtagtaggtg
      361 tcgaggtcgg cgggtgcgat cgaggccacc ttgcgtgtcc ccatcgtagg gacgaggcgc
      421 ttggcgatga tgcgctcgta ctcgcgagca gtggtggggg agagtcgggc ttccttctgc
      481 tcccaccacc tctcgagcag gtgctcgagc gtgtggctct gcgccgcgcc gcggtcgtcg
      541 tcggcctcgg ccacgaggcg ggcgagttcg cgctccgcct gccgcttggg cccctcgaag
      601 gacgcgcttc ggcgccgccg ccggccggtc accgggtcgg gcggcagcgg gacccgcagc
      661 tcccaccggc cgggagtctt ctcccggaga ctgcctcgca tgcgggggac cgtagcaacg
      721 atccgagaaa tttgggggga ttttgggggg atgggccgga gtcgaagctc cggcccatcc
      781 gcttcgcagg cctgtgacct gcgctttttc ctgtaccccc cctgggactc gaccccagaa
      841 cctgcggatt aagagtccga cgctctaacc agttgagcta gaggggcagg tggtccgctc
      901 ccggccgggg ccgggggcgt atgtgagcgg tcgaatctct cgccgcgtgg ggtgaccgag
      961 gggacttgaa cccccgacct ccagggccac aacctggcgc tctaaccgaa ctgagctacg
     1021 gccaccaagg agcggcgatc ctaggccgca gcgacgtcgc cgacgaaacg agaccggcag
     1081 acgtggccgg gtacccttcc gccccggcct ccgtagctca actggagaga gcatccggtt
     1141 tctacccgga cggttgcggg ttcgactcct gccgggggca cgcagacacg cgccggcgca
     1201 gccggctcag gtggtccggc ggcgcagcgc gagcccggcg aggccgatga ccacggcggc
     1261 cccgatggcg gacgtgccgc cggtgtcggc caggccgtcc tcgaggtcgc gagggacggt
     1321 gcggccgctg tcgctgtcga ggccgctgcc gccgcccgat ccggtcgtgc cacccggtcg
     1381 gctggtggtg gtcgtggtgg cgccggcgga gcccacggct gcggtcgtgg tgggggcggt
     1441 gcccgtcgcc tcggtggtcg acgtggagga cgtgccatcc gacgccggcg tcgaggtcgt
     1501 ctcggtctcg gtcgccgtgt ccgtgtccgt ctctgtttcg gactcgtcct cggtctgcgt
     1561 gtccgtgtcc gtctcggtgt cggtctcggt tcccgcctcg tcctcggtct gcgtctcggt
     1621 ctcggtctcg gtctcggtca gctcgggctg ggccgtctcg gtgtcgccgt cgtcgtcggc
     1681 caggcccccg ggctgcacca gggccacgac gaggcccacg gcacccagca gcagggcggc
     1741 cgccagcagg agcttgggga ggggcttcac gtcagggagg tccttcgggg ggacgaggtt
     1801 tcggcgccgg cgtgctgcgg agggtggggc ggaccgacgg gcggattgga gcgggtgacg
     1861 ggaatcgaac ccgcgtgacc agcttggaag gctggggctc tgccattgag ctacacccgc
     1921 agacgggtca cgacgctagc cgcgcgccgc tccggtcccg atttccgcgg tcccgggtga
     1981 ccccggggat ccccgacagc cggggcagga gggcgcgacc ggagcggcac cgccacgccc
     2041 cgcgccacac gcccgcgcag tacgccgcct ggtccagggc gtggatcagg gtccagcgca
     2101 ccgggtcgac ccccggctgc gggccggcgg cgcgccggtc gaggagggcg ggcaccacgg
     2161 cggcggccag cgtcgcccgt cgcacccggc ggctgcggag ggacccgacg agcgcgaggg
     2221 gcaaccaggc ccgccagacc gaccggccca gccacgctcc cgcccgcagc gtgccgccgc
     2281 cggcgagctc gagcgagctc cgcaccggtt cgtccacccg tccccgcagc ttgggcacga
     2341 gggccaggga cgaaccgacc gcgacgccgg caccggccag cgcaccccgg cgaccgccga
     2401 tcacggccag gacccacgcc agcaggctcc acgcgttcag ctcgaccggc gcgaccgcgc
     2461 cgggatggcg ctggtcgagc ggcgcggccg ccgagccgta ggccatccgc tggcgcacca
     2521 aagcgcccca gctgccgcgg gggcggtgct cgacctcggc ggcgggctcg tagcgcacgg
     2581 tccagccggc ctccacggcc cgccacacca ggtcgacgtc ctcgccgacc cgcatgtcgg
     2641 gctcgaaccc atccagctgc tcgagcagct cccgccggca gacgaccgtc gccgtcggga
     2701 cgtaggacac gcgcccgcgg ggcacgaccc gtgcctcccg cgccccgagg tcgagcgggg
     2761 agcggtgccg ctcgtagcgc tcgagcagcg acggtccggg ccggctgcgg acccggggcg
     2821 cgaccaagcc cacggccggg tcggcgaggt gggcgagcag cgggtcgagc cacccgtcgc
     2881 ccagatggac gtcggcgtcg acgaaggcca cgacgtcgcc ggacgaggcc cgccagccgg
     2941 tgtcccgggc cgcaccgggg ccgagggagg agtcgtggcg cagcacctcg gcgcggccgg
     3001 cgaccgcgcc gggtacggcg gcgggatccg gggtgccgtc gtcgaccacc acgacttcgg
     3061 tgccggccgg cacgtggagg gcgtcgagca gggaggcgag ctcggcggcg tggtcacgga
     3121 ccggcacgac caccgacagc gtgagcccgg gggtgcgggc cgggcgcggg tggaccatgc
     3181 cggcgtcgag gaggcggcgg gccaccaggg cctcgggtcc ggtggtcggc acggccccgc
     3241 cggcgaggac ccggtcgacg gcagcggcgc cggcatcgct gaagcgcagg aggcgcatcg
     3301 gcgcaccccc gaggagcgag cggccgtccg cgctgcgacg ggtctcggcg tccagggcga
     3361 gcgcgatatg ttcggggaac ccctcggcca cggtcctcct cgagcgacgg agtggcgagg
     3421 ctagccctgc ccgcacgtcc cccagaggtg tccgatgacc gtcaccgagc ccaccaccga
     3481 gtcctccgcc gaggccgagg accaggagca ggccaccgag gagctcgtga ccgacgacgt
     3541 cctcgtcgag gagatctcca tcgacgggat gtgcggcgtc tactaggcgc ccgccctcga
     3601 cccgatggcg ttcgatcccg ctgaccgcta ccggctcagc ccccgggtgg gcctgcggcc
     3661 cgagcccttc ggcgccctcg cctaccacta cgacacccgg cgcctggtct tcctcaagtc
     3721 caacgacctc gtcgccctgg tgcgctcgct cgagcagcac gacagcgcgg ccgccgccat
     3781 cgacgccctg gtcgacccga ccaccggccg ccgatccgcc tacgtgaccg cgctcgcctc
     3841 gctctgcgag tccggcgtgg tcgaacccct gctggagccc gccgcatgag caccatcccg
     3901 aagaccccgc cgctggtcga gcagctcaag ggcggcctcg atgcccccat ctgcctcacc
     3961 tgggagctca cctacgcctg caacctggcc tgcgtgcact gcctctcgag ctccggtcgc
     4021 cgggacccga aggagctcag caccgacgag gccaaggacc tcatcgacca gctggccgcc
     4081 ctccaggtgt tctacgtgaa catcggcggc ggcgagccga tgctgcgcac ggacttcttc
     4141 gagatcgtcg agcactccgt caccaacggt gtcggcgtga agttctccac caacggcacc
     4201 tacctcgacc gggccgccgc cgagcgcctc gtcgagctgc cctacctcga cgtccagatc
     4261 agcctcgacg gcatgacccc cgaggtgaac gacgccgtcc ggggcgaggg ctcccacgcc
     4321 aaggccatcg aggccatggg ccacctgcgc gacgtgggct tcgaggactt caagatctcc
     4381 atcgtctgca cccgccacaa cgtcgaccag ctcgacgagt tcaaggcgct ggccgacgac
     4441 cacggcgccc agctgcgcat cacccgcctg cgcccctcgg gtcgcggcgt ggacagctac
     4501 cacgacctgc gcctcaccaa cccccagcag cgccaggtct tccactggat cctcgaccac
     4561 cccgacgtgc tcaccggcga ctcgttcttc cacctgaacg cgctcaccac cgagaccggc
     4621 ggaccgaacc tgcccggcct gaacatgtgc ggtgccggcc gggtcgtgtg cctcatcgat
     4681 ccgatcggcg acgtctacgc ctgcccgttc gtgatccacg acgagttcct cgccggcaac
     4741 gtccgcgacg ccggcgggtt cgcccacgtc tggcaggagt cgtcgctgtt caccgagctg
     4801 cgcgagccca ccagcgccgg ggcctgcgcc tcgtgcgggt cctacgacgc gtgccagggc
     4861 gggtgcatgg ccaccaagtt cttcaccggc ctgcccctcg acggaccgga tccggactgc
     4921 atccacggcc acgccgacga ggccctcgcc ggcatcgccg ccggctcggc cccccggccc
     4981 tcacaggacc acagccgccc gggcgcggcc gtgcccgtcg ccctcacccg caagtcgtag
     5041 cgaactggca gcggtgacgg aacgcgtcgc gttccgtggt cgctgccaga tccctactcg
     5101 gtcgcctcgg cctggcggac ggagtcggag aagtcgtcgg gcacgacgat gtcgagcggg
     5161 ccgaggtcgg cgagcgtgcg cttgccgagg ccgagcatgg ccgagtcgac gccgttgcgg
     5221 agcacgtcga gcacgttctc gacgccgggc tggccaccga cggccagggc ccacagcgac
     5281 gcccggccga tcatgacggc cttggcgccg agggcgacgg ccttgacgac gtcgctgccc
     5341 cggcggatgc cgccgtccat cagcacctcg atctggtcac cgaccgcctc ggcgatgagc
     5401 ggcagggccc ggatcggggc cggggtggtg tcgaggttgt tgccgccgtg gttggagacg
     5461 ctgatcgcgc tgtagccggc gtcgacggcc cgcttggcgt cgtcgacgcg catgatgccc
     5521 ttgagcatga agggcccgtc ccactgctcc cggagccagc gcagatcgtc ccagctgggc
     5581 gggggcgtgc ccatccactg cccgtaggcc tcgaagaacc cgggaggggc ctcgccctcg
     5641 gggaccatgt tgggcacgtg cagatcgggg agcgagcggt tctcgagggt gtacttgccg
     5701 tagcgccagg cgtatcgcgg gctggtgagc agctcggggg agtgcttgat cgacgtcttg
     5761 aggtcgagga cctcggggat gtcggggctg ccccagtcgc ggccgtggga gaaggaccag
     5821 tcgagcgtga cgatcagccc ggcgacgccg gcgcgcttgg cccggtccat gcgggaggtc
     5881 atctgctcgc gggtgccgca ccagtagatc tggaagaacg tctgggggtt ctcggccatg
     5941 acggtctcga ccggggtgga cccgaagttg gacaggccga gggccgtgcc ccggttggcc
     6001 gacgcccggg cgatgccgat ctcggcctcg gggtgcacca tctggacgcc ggtcggggag
     6061 atgaggaccg gcatcgagat gctctggccc atcacctgcg tggactggtc ccgctcggcc
     6121 ggcttgtcgg cgacggtcgg cacgaagccg agctcggaga acgccttggt gttgtcggcc
     6181 agggtgtggc cggcctcgga gccggccttg agggcgccga agaccgatcg ggggaggatc
     6241 ttctcggcgc gctgccgggc gatctcgacg ctctcgaacc acttgtgtgc catctgtcca
     6301 cctcgtgccg gtcggagcga tcatgttggc atgaccgctc cgtacccaga gaagcgacag
     6361 ctcggctcgc agacctggcc ggaggtggcc gagacgccac cccgggtcct gctcgtcccg
     6421 atgggcagct gcgagcagca cggcccgcac ctgcccctcg acaccgacac ccgcatcgcc
     6481 gtcgccgtcg ccgagcgggt cgccgcggga cgggacggcg tggtcgtcgc gccggcggtg
     6541 gcgtacggct cgtccggcga gcaccagggc ttcgccggca cgctgtcgat cggcaccgcg
     6601 gcgctgcgtt cggtgctgat cgagctcggg cggtcggcgt tcccggccgc cgatccgggg
     6661 gcgttcgcgt cgctggtctt cgtcaacggc cacggcggca accacgaggc gctccgcgac
     6721 gcggtcgacg tgctggtggg ggaggggcgg tcggtgtcgg cgtggtggcc gtcgatcgcc
     6781 gggggcgacg cccacgccgg acgcaccgag acgtcgcttc tgctggccat cgccggcgac
     6841 gtggtcggct cggcgcgtcc cgtcggcgcg accgaaccgc tggcggcgct gctggagccg
     6901 atgcgggccg gcggcgtcgg tgcggtggcg ccgaacggcg tcctgggcga cgcgaccgat
     6961 gcctcggcgg aggagggcga ggcgctgctg accgccctcg tcgacgacct ggcggggcga
     7021 ctctcggttt cttgaccgaa cagtcaggtt tggcatgaca acggcgcggc gcgggcctag
     7081 gttggcgacc gctcatccga aatcgcacca cgaacccggg ggaacccaac atgaagaccc
     7141 gcgccgccat cctgtgggag aagaaccaag actggagcgt cgaggagatc gatctcgacc
     7201 cgccccagac cggcgaggtg ctcatcaagc tggtcggctc cggcctgtgc cactccgacg
     7261 agcacctgcg caccggcgac atggtcgtcc cccaggagac cgccgacgcc ctcggcctca
     7321 agcagttccc gatcatcggt ggccacgagg gcgccggtga ggtcgtcgag gtcggcccca
     7381 acgtcaccgg cctcgaggtc ggcgaccacg tggtgctcgg cttcatcccc gcctgcggca
     7441 agtgcccgtc gtgctcgagg ggtcggtcga acctgtgcga ctacggcgcg ttcctgctcg
     7501 ccggccgcca gatcgccgac ttcaccgccc gccaccacgc cgccgacggc accgacctcg
     7561 gcctcatgtg cctgctcggc accttcggcg agtacacggt cgtcaacgag gcgtcctgca
     7621 tcaagatcga gaaggacatc cccctcgaca aggccgccct cgtgggctgc ggcgtcacca
     7681 ccggctgggg ctcggccacc tacgccgccg acgtccagcc gggcgagacc gtcgtggtcg
     7741 tcggtgccgg cggcatcggc atgaacgcga tccagggtgc ggccatgaac ggcgcccgcc
     7801 acatcgtcgc cgtcgatccg gtcgagttca agcgcgagca ggccatggtc ttcggcgcca
     7861 cccacacggc cgcctccatc gaagaggcca ccgagctggt cggccagatc acctggggcc
     7921 agaacgccga gaaggcgatc atcacggtgg gcgtcggcga cggcgacctc atcgccccgg
     7981 tcatgggcct caccgccaag ggaggtcgcg tcgtgcacac ctccgtcgca cccatggagc
     8041 gcaacgacgt cgccctcaac ctgttcgacc tgaccctgca gcagaagcag ctcgtcggct
     8101 cgatcttcgg ttcggccaac ccccgctacg acgtcccccg cctgctgcgc ctgtaccagg
     8161 agggcaagct caagctcgac gagctcatca cccgcgagta cgacctcgcc gacatcaaca
     8221 agggctacga cgacatgaac gacggcaaca acgtccgtgg gttcgtgcgc tacaaccagt
     8281 cctgaatgac ccggtccgac cacggcttcg accgctcgct catcgagagc gcggcggtcg
     8341 aggtcgtcgg ccggcggcgt gagctcgagc tcgtcaccgc cgccctcgct gcgtcccgcc
     8401 acgtcctgct ggaagggccg cccggcaccg gcaagtccac gctcctgcgg gccatcgccg
     8461 agcgcgccgg cctcgagctc gtcttcgtcg agggcaacgc cgagctgacg ccggctcgcc
     8521 tcgccggcca cttcgacccg gcgcgggtcc tcaccgacgg ctacgccgag gacgtcttcg
     8581 tcgacggtcc gctcctgcgg gcgctgcgcg acggcggcct cctctacgtc gaggagatca
     8641 accgggtccc cgaggagacg ctgaacctcc tcatcaccgt catgtccgag ggggagctca
     8701 ccgtgccccg cctcgggcgc gtcgtggccg cacccgggtt ccggatggtc gcggcgatga
     8761 acccctacga cgccgtgggc accgcccgca tctccggcgc cgtgtacgac cgggtctgcc
     8821 gggtgtcgat gggctaccag tccctcgacg acgagctggc catcgtcgtg cacgaggcca
     8881 agaccgcggg cgccaccgac gcggccggcg acgacgaatt cgtgcggcgc gtcgtcagcg
     8941 tcgtgcgggg cacccgtgag caccccgacg tgcgcatcgg gtcctcggtg cggggcgcca
     9001 tcgacatggt cctcatcgcc gacgagctcg ccggcctgcg agagaccgat gcatccgagc
     9061 ccgacacggc cctcgatgcc gcctgcatgg cgctctccgg tcgcatccgg gtccacgagt
     9121 cctgccaccg cacccccgag gacatcgtcg ccgagctctg ggacctgtac gccaccaccg
     9181 tcgacctcgg cgagggcggg ggcgacggcg aggacccggg aaaaggctga gcctcccgga
     9241 cggctccggg agggatgagg accgggagcg caccctcgac gaggaggcga cccaggagga
     9301 gctgaccgag tcctcccgcc gcacgacctc ccgccgggag ctgtcccagc acgacgactt
     9361 cgacgaggtg tcgcccgagc tcggcgtcct cgacgagagc gccatcgagg agtcgctggc
     9421 cgatcggccc gacgagaccc tcgccatgct cgccgacatg accggcgcca ccgacgagcg
     9481 cctccgtgcc ctcgcccgcc gcctcgccgg ccggatcgtc atcgggatgg cccgggccga
     9541 tgcggctcgc cgccggggca tcggcaagat gcgctcgctg ccgatggacg acaccggcgg
     9601 tgacatcgac atggacgcca gcatcgaggc cctgcagctc gcgtcggcca ccggcaccgc
     9661 cccccgggcc gacgagctga ccgtgcgcac gtgggcccgt ccggcggtgg cgatctgcct
     9721 cgtcgtcgac cgctcgggct cgatgagcgg tgaccggctc gccgcctctg ccatcgccgc
     9781 cgctgcggtg gccgagcgcg cccccgacga cttctcggtg ctggccttct cggacaaggt
     9841 gatgatcatc aaggggcaga gccagcgccg ctcggtcgac gacgtcgtcg aagacgtgtt
     9901 ccggctccgt ggccacggcc cgaccgacct cgggctggcg ctgcgggtcg ccgccgacca
     9961 gctcgcctcg tccggcgccc agcgcaagcg cgtcgtgctg ctgtcggact gccggcccac
    10021 caagggcacg gagcccgagc gcgacgccgg cgccatcgac gagctccacg tgatcgcacc
    10081 ggccgaggac gccgccgacg ccgaggcctt cgcccgggcc gtcggggccc gcttcgcggc
    10141 tctctccggt ccgagccaga tcccgtcggt cttcgcccag ctggccgact gagccccgga
    10201 gcggtgagcc caggggcaca ctgaccgggt ggctgccacc gacaccggga tgaccgacga
    10261 gcgactcgcc gacctgcgtc ggtggaacct gggcctcacc gtcctgcacg tcgcgcaggc
    10321 cgtcgccgtg ctggtgctgg ccagcgactt cgccatcacg gtgacgtcgt cgttcccgac
    10381 cggtccgccc ggggccgccc cgccggcgcc cgaggcgctc ttcgacatcc cgatcggggc
    10441 agccgtcgcc gtcttcctcg cgctcgccgc cctcgaccac ctcgtcaccg caaccatcgg
    10501 gcgccgcacc tacgaggccg acctgcggcg ggggatcaac cggttccggt gggtcgagta
    10561 ctccttcagc gccacgctga tgatcgtcct catcgccagc tacagcggca tcaccgagat
    10621 cacggcggtc atcggcatcg ccggcgccaa cgtggccatg atcctcttcg gctggctgca
    10681 ggagcgcatg aacccgcccg gccggtcgtc cacgtccatg ctggcgttct ggttcggatg
    10741 cgtcgccgga gtggcgccgt ggatcgccat cgccgtcaac atcttcggcg ccgacaccgt
    10801 gccgggcttc gtgtacggca tcttcgtcgc ccagctcgtg ttcttctcga gcttcggcct
    10861 gaaccagtgg ctgcagtacc gggggatcgg tccgtggtcc gactacgcgt tcggggagaa
    10921 ggcgtatctg gtgctcagcc tcggcgcgaa gtcggtgctg gcctggcaga tcttcggcgg
    10981 gtcgctggcc ggctgagcac atggcgcccg gtcgggtcgg gtacgtgctc ggcatggctg
    11041 atgaactgaa cggcaagacg atcgccttcc tcgtcgccca ggagggcatc gagcaggtgg
    11101 agctgaccga tccctggaag aaggtggagg aatccggcgg ccagccgatc ctggtggcac
    11161 cggaggccgg caccgtccag gcgttcgacc acctcgacgc cgccgacacc ttcgacgtcg
    11221 accgcaccac cgctgacctg cgggtggacg acatcgacgg cgtggtcctc ccgggcggcg
    11281 tcgccaaccc cgaccagctc cgcaccgacg aggccgccgt ctcgttcctg cgggacttcg
    11341 tgcagtccgg caagcccgtc gccgtgatct gccacggccc ctggaccctc gtcgaggccg
    11401 gcgtcctcga ggggcggacc ctcacctcgt ggcccagcct gcagaccgac atccgcaacg
    11461 ccggcggcac ctgggtcgac caggaggtgt gccgcgacgg caacctcgtg agcagccgca
    11521 accccggcga cctgccggcg ttcaccgacg ccatggtgga gctcttctcg tagcgcgcgg
    11581 gtggtcgggg agacaggatt cgaacctgcg accccctgct cccaaagcag gtgcgctacc
    11641 gggctgcgcc actccccgtg gaccggcggg gaactctagc ggccgcccac ccatgcgtcc
    11701 cggcccgggg cccgtcagta cactcggccg ctcctatgag cacgtccccc acccgacaga
    11761 agctggtcac ccgatgaact cgacggtcga ggaactcgat ggcaaca
//
LOCUS       DATISU010000417         6286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_640729, whole genome shotgun sequence.
ACCESSION   DATISU010000417 DATISU010000000
VERSION     DATISU010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640729"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VLR27_16530"
     CDS             complement(<1..320)
                     /locus_tag="VLR27_16530"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HSP05115.1"
                     /translation="MHANAARVVAAAGAEGLTIEVVEYPDGTRTAAEAAAAVGCEVAR
                     IVKSLVFTVDGTPVVALVAGDARLDEALLAEAAGGAVGRADADVVRSATGYPIGGVPP
                     FAHAS"
     gene            379..867
                     /locus_tag="VLR27_16535"
     CDS             379..867
                     /locus_tag="VLR27_16535"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSP05116.1"
                     /translation="MRAWTVAGGLIEHEGRLLLVQNQRRDGSTDWTTPGGVVDPGEEI
                     LEALSREVLEETGLAVTGWEGPLWQVEAEAPDMGWTLRVEVHRATGFEGRIMLDDPDG
                     IVIDAAWLDLAGCHDCLSTSWLPAREPMLAWLGEKWLEPRTYRYRLEGVDRADANVIR
                     LD"
     gene            870..2183
                     /locus_tag="VLR27_16540"
     CDS             870..2183
                     /locus_tag="VLR27_16540"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HSP05117.1"
                     /translation="MPTSGGAGRTILHVDMDAFYASVEQLRRPELRGKPVVVGGSGRR
                     GVVAAASYEARAFGVRSAMPSMRARTLCPHAVFVDGDHAHYAEVSSRVMAIFASMTPL
                     VEPLSLDEAFLDVTGALRLHGDGPTIAAEIRARVLAEEGLTCSVGVATSKFIAKLATE
                     EAKPSASVRGPVPGTGVAVVRPGDERVFLRPLPVGALWGVGPRTLEKLHALGVRTVGQ
                     LERLPLDQLVRSLGDAHGRHLHDLAHAVDPRPVVPDQRPKSISHEETFAEDIDDPRAL
                     RTELVRMADAVARRARHQDVSGRTVAIKVRFGDFTTISRSITLAQAVDTGPAIARAAT
                     QLLDQVDIAPGVRLFGVGLANLADDAPHQLSLDDLTRAAEAPAADGAAWTAVTEAVDA
                     VRARFGDDALLPAALAERGSLRAKRPGEQQWGPHERRASSPDDGV"
     gene            2285..2668
                     /locus_tag="VLR27_16545"
     CDS             2285..2668
                     /locus_tag="VLR27_16545"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HSP05118.1"
                     /translation="MPLSEDEERILKDIEREFYENDPAFAREVRETTLYKHAWRNIKL
                     SLVGFVAGLVLLVAALSSSYLLAFGGFLVMLGCALVIERNARKMGRAGLETMTKNLKS
                     SNGLKDYLGSSSEKMRERFRRDEEQ"
     gene            complement(2688..4985)
                     /locus_tag="VLR27_16550"
     CDS             complement(2688..4985)
                     /locus_tag="VLR27_16550"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HSP05119.1"
                     /translation="MTAAAVGLRPGNRTFPVQAVAEVAFALVSLATVISLRRVFAGGE
                     WFVPLLVHVVVAHATVTVLRRRQVAAPVAAALAAVAAVVVIAWVHAGQATTFGIPTLS
                     TIDVLLTSVNDAFAQFSDIKAPTAPLEGFLVASAVAVWASAVVGDWAAFRADATVEAV
                     LPAAALFGVASVLGADVGRILLAGIWIATVLSFVLVRRADRLGRSSTWVGDHRSRGPQ
                     ALVVLGVSLAVVATVVAAVVGPRLPGAESEAVVSLTDLGDDAPGTRVTVSPLVDIRSR
                     LVQQANVEVFTVRSDQRAYWRLTSLDRFDGSIWSSNGSYGKSSGDLDDGVPVASERLV
                     FDQQFTISALSQIWLPAAYEPQQVEVDEPVRYDEVSGTLIVDTEIPTSDGTAYAVRSA
                     LPQHLPDALAGASAEIPTDIAERYLELPGDFSPDIRALAAEVTAGSTSGYEQAMRLQD
                     FFRTQFSYDLQVDLEHGIGDMQDFLFAVRAGYCEQFAGSFAAMARSLGLPARVAVGFT
                     PGVADPADPTLYRVRGEHAHAWPEVYLGEYGWVAFEPTPGRGAPFAEQYTGVPEQQTQ
                     SGGDGGSATTVPATPSTEVASPNTSIPGQTPPTFPDDPGLDLGTGGEAEPEPNPWPLR
                     IVLALAIALAAAVLYLLAVVAFTAGARLLRRRRADSAVARIALAWDESLAAARRAGVG
                     VRPSATQRQAADEIGRRIPAVDEPIRALASTVEASAYSPTAPDDDAGDVALALADRVG
                     DGTRAAMTPRDRFRTRFDPRLLRRR"
     gene            complement(4982..6169)
                     /locus_tag="VLR27_16555"
     CDS             complement(4982..6169)
                     /locus_tag="VLR27_16555"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSP05120.1"
                     /translation="MRPTRTGLLVGIASAVLVVAGRLLGLAELYAIGAAVGLLALVCG
                     LWVGLRRLDIVVTRSVRPSRVHVGNPCTVAIQVRNRAGRATPVLRLLDPVTGTAGADL
                     LLAPIGLRRTTDVAYRLPTTKRGLVTVGPMQVAMSDPFGLASTRNPAAPAVEVTVLPR
                     VDDIPPLPRSVGPDPDGTAETGSLGRSGEDFAALRPYIVGDDLRRVHWPSSARTGDLL
                     VRQHDVPWQGRVCVVLDLRRQATDEVTLERAISAAASVLRTHLRRGDHVRLVTTTGVD
                     SGYGVGASHLDGLLEYLAMAGRSSSGSLRMAMELVERGASGAVVLVSGTLSGADMELF
                     ERSGSAVSLRRIVRFDGAQRVTTPRRTEVLGVGPGEEFAQAWSESTGATAGRRRRRRQ
                     VVS"
BASE COUNT          856 a         2272 c         2411 g          747 t
ORIGIN      
        1 gaggcgtggg cgaacggcgg cacgccgccg atggggtagc cggtggcgga tcgcaccacg
       61 tcggcgtcgg cccggccgac cgcgccaccg gcggcctcgg ccagcagggc ctcgtcgagg
      121 cgggcgtcgc cggcgaccag cgccaccacc ggcgtgccgt cgacggtgaa cacgagggac
      181 ttcacgatgc gggcgacctc gcagcccacg gccgcggcgg cctccgccgc ggtgcgggtg
      241 ccgtccgggt actcgacgac ctcgatggtg aggccctcgg ctccggcggc agcgacgact
      301 cgggcggcgt tggcgtgcac gacccgcatc ctgcccgacc ggacccggtc gccgacacgt
      361 cgctggcaga ctggcggggt gcgtgcgtgg acggtggccg gaggactgat cgagcacgag
      421 ggccgcctcc tcctcgtcca gaaccagcgc cgcgacggca gcaccgactg gaccacgccc
      481 ggcggcgtcg tcgaccccgg tgaggagatc ctcgaggcgt tgtcgcgcga ggtcctcgag
      541 gagaccggcc tggccgtcac cggctgggag ggcccgctct ggcaggtgga ggccgaggcg
      601 ccggacatgg gctggaccct gcgcgtcgag gtgcaccggg ccaccgggtt cgagggccgg
      661 atcatgctcg acgaccccga cggcatcgtg atcgacgccg cctggctcga cctggccggc
      721 tgccacgact gcctgtccac ctcctggctc ccggcgcgcg agccgatgct ggcctggctg
      781 ggggagaagt ggctcgagcc ccgcacgtac cggtaccggc tcgagggcgt cgaccgggcc
      841 gacgccaacg tgatccggct ggactgacgg tgccgaccag cgggggagcc gggcggacca
      901 tcctccacgt cgacatggac gcgttctacg cgtcggtcga gcagctgcgg cggcccgagc
      961 tgcgcggcaa gcccgtggtc gtgggcggat ccgggcggcg gggcgtggtg gcggctgcct
     1021 cctacgaggc ccgggccttc ggggtccgct cggccatgcc ctcgatgcgg gcccggacgc
     1081 tgtgcccaca cgccgtcttc gtcgacggcg accacgccca ctacgccgag gtgtcgtcac
     1141 gggtgatggc gatcttcgct tcgatgaccc cgttggtgga gccgctcagc ctcgacgagg
     1201 ccttcctcga cgtgacgggc gccctccgcc tccacggcga cggccccacc atcgccgccg
     1261 agatccgcgc ccgcgtcctc gccgaggagg gcctcacctg ctcggtcggc gtcgcgacgt
     1321 cgaagttcat cgccaagctg gccaccgagg aggccaagcc gtccgcctcg gtgcggggcc
     1381 ccgtccccgg gaccggcgtc gccgtggtcc ggcccggcga cgagcgggtc ttcctgcggc
     1441 ccctccccgt cggcgccctc tggggcgtcg gaccccgcac gctggagaag ctccacgcac
     1501 tcggcgtccg caccgtcggc cagctcgagc gcctcccgct cgaccagctc gtccgatcgc
     1561 tcggcgacgc ccacggccgc cacctgcacg acctcgccca cgccgtcgac ccccgcccgg
     1621 tggtgcccga ccagcgcccg aagtcgatca gccacgagga gaccttcgcc gaggacatcg
     1681 atgatccccg ggcgctgcgc accgagctcg tccgcatggc cgacgccgtg gcccgccggg
     1741 cccggcacca ggacgtgtcg gggcgcaccg tggccatcaa ggtgcgcttc ggtgacttca
     1801 ccaccatctc ccggtcgatc accctggccc aggccgtcga caccggaccg gccatcgccc
     1861 gggcagccac gcagctgctg gaccaggtcg acatcgcacc gggcgtgcgc ctgttcgggg
     1921 tggggctggc gaacctggcc gacgacgccc cccaccagct cagcctcgac gacctgaccc
     1981 gggccgccga ggcgcccgca gccgacggtg cggcgtggac cgcggtgacc gaggcggtcg
     2041 acgccgtccg ggctcgcttc ggcgacgacg ccctcctccc ggcggcgctg gccgagcggg
     2101 gctcgctgcg ggcgaagcgg cccggcgagc agcagtgggg tccccacgag cggagggcat
     2161 cgtcgcccga cgatggggta tgagggtggc cgacgcaccg ctccgcgcgt tgttgctcgc
     2221 cgacacgtgt gcgatgatga cgaccggagc aggcgtcccg acgaggacca gaagaggtga
     2281 gcaggtgccc ctttccgagg acgaggagcg catcctcaag gacatcgagc gggagttcta
     2341 cgagaacgac ccggccttcg cccgtgaggt gcgcgagacc acgctctaca agcacgcctg
     2401 gcgcaacatc aagctctccc tcgtgggctt cgtcgccggc ctcgtgctcc tggtggccgc
     2461 cctctccagc tcctacctcc tcgccttcgg cgggttcctc gtcatgctcg gctgcgccct
     2521 cgtcatcgag cgcaacgccc gcaagatggg tcgcgccggc ctcgagacga tgaccaagaa
     2581 cctcaagtcc agcaacggct tgaaggacta cctcggctcg tcctcggaga agatgcgcga
     2641 gcgcttccgc cgcgacgagg agcagtagca gcccacccgg tgccggctca gcgccggcgc
     2701 agcaggcggg ggtcgaagcg ggtgcggaac cggtcgcgcg gcgtcatcgc cgcccgggtg
     2761 ccgtcgccga cgcggtcggc cagggccagc gcgacgtcac cggcgtcgtc gtccggtgcg
     2821 gtgggcgagt aggcgctggc ctccaccgtg gacgccaggg cgcggatcgg ctcgtcgacc
     2881 gcggggatgc gccgtccgat ctcgtccgcc gcctggcgct gcgtggctga cgggcgcacg
     2941 ccgacgccgg cccgtcgtgc ggcggccagc gactcgtccc acgccagcgc gatgcgggcg
     3001 acggccgagt cggcccgccg ccggcgcagc agtcgggcgc cggcggtgaa ggccaccacg
     3061 gcgagcaggt agaggacagc cgccgccagt gcgatcgcca gggcgagcac gatccgcagg
     3121 ggccacgggt tcggctcggg ttcggcctcg ccgccggtgc cgaggtcgag cccggggtcg
     3181 tcggggaagg tgggcggggt ctggccgggg atggacgtgt tcggcgacgc cacctcggtg
     3241 gacggcgtcg ccggcacggt ggtggccgag ccgccgtcgc cgccgctctg ggtctgctgc
     3301 tcggggacgc cggtgtactg ctcggcgaag ggcgcgcccc ggcccggcgt gggctcgaag
     3361 gcgacccagc cgtactcgcc caggtacacc tcgggccagg cgtgggcgtg ctcgccccgc
     3421 acccggtaca gggtcgggtc ggccggatcg gccacccccg gcgtgaagcc gacggccacc
     3481 cgggcgggca gcccgaggct gcgggccatg gcggcgaagg atccggcgaa ctgctcgcag
     3541 tacccggcgc ggacggcgaa gaggaagtcc tgcatgtcgc cgatgccgtg ctcgaggtcg
     3601 acctggaggt cgtagctgaa ctgggtccgg aagaagtcct ggaggcgcat cgcctgctcg
     3661 tagccgctgg tgctccccgc ggtgacctcg gcggcgaggg cgcggatgtc gggggagaag
     3721 tcgccgggta gctccaggta gcgctcggcg atgtcggtgg ggatctccgc cgaggccccg
     3781 gcgagggcat cgggcaggtg ctggggcagg gccgagcgca ccgcgtaggc ggtgccgtcc
     3841 gaggtcggga tctcggtgtc gacgatgagc gtgccggaga cctcgtcgta gcggacgggc
     3901 tcgtcgacct ccacctgctg gggctcgtac gccgccggca gccagatctg ggacagggcc
     3961 gagatcgtga actgctggtc gaagacgagg cgctccgacg cgaccgggac gccgtcgtcg
     4021 aggtcgcccg agctcttccc gtacgacccg ttcgacgacc agatgctgcc gtcgaagcgg
     4081 tcgagggagg tcaggcgcca gtaggcccgc tggtcgctgc ggaccgtgaa cacctcgacg
     4141 ttggcctgct ggacgagccg agagcggatg tccacgagcg ggctcaccgt cacgcgggtg
     4201 cccggcgcgt cgtcgcccag atccgtcagc gagacgaccg cttcggactc ggcgccgggc
     4261 aggcgggggc cgacgacggc ggcgacgacg gtggcgacga cggcgaggct gacgccgagc
     4321 acgaccagcg cctggggtcc ccgggagcgg tggtcgccga cccaggtcga cgagcggccc
     4381 aaccggtcgg cccgccggac gagcacgaag gagagcaccg tggcgatcca gatgccggcg
     4441 agcaggatgc ggccgacgtc ggccccgagg accgacgcga caccgaacag ggctgcggcg
     4501 ggcagcacgg cctcgaccgt ggcgtcggcg cggaaggccg cccagtcgcc caccacggcg
     4561 ctggcccaca ccgcgaccgc cgacgccacc aggaacccct cgagcggcgc cgtcggtgcc
     4621 ttgatgtcgc tgaactgggc gaacgcgtcg ttgaccgagg tcagcagcac gtcgatggtg
     4681 gacagcgtgg ggatcccgaa cgtcgtcgcc tggccggcgt gcacccacgc gatgacgacc
     4741 acggcggcca ccgcggcgag cgcggcggcg accggcgctg cgacctggcg ccggcgcagc
     4801 acggtgacgg tggcgtgggc gaccacgacg tgcacgagga ggggcacgaa ccactcgccc
     4861 ccggcgaaca cccggcggag gctgatgacg gtggccaggc tcacgagcgc gaaggcgacc
     4921 tcggccaccg cctggaccgg gaaggtgcgg ttgccgggac ggagcccgac ggcggcggcg
     4981 gtcacgagac cacctgccgg cgacgccgcc ggcgaccggc ggtggcgccg gtggactcgc
     5041 tccaggcctg ggcgaactcc tcgcccggac cgacaccgag cacctcggtg cgccgggggg
     5101 tggtgacccg ctgggcgccg tcgaagcgga cgatgcggcg cagggagacg gcgctgccgc
     5161 tccgctcgaa cagctccatg tcggcgcccg agagcgtgcc gctcaccagc acgaccgcgc
     5221 cgctggcacc gcgctcgacc agctccatcg ccatgcgcag cgaccccgag ctgctccggc
     5281 cggccatggc caggtactcc agcagaccgt cgaggtgcga ggcacctacg ccgtagccgc
     5341 tgtcgacgcc ggtggtggtg acgagccgga cgtggtcgcc gcggcgcaga tgggtccgca
     5401 gcaccgaggc ggccgcggag atcgcccgct ccagggtcac ctcgtcggtg gcctggcggc
     5461 gcaggtcgag caccacgcac acccgcccct gccaggggac gtcgtgctgg cgcacgagca
     5521 ggtcgccggt gcgtgccgac gacggccagt gcacccgtcg caggtcgtca cccacgatgt
     5581 aggggcggag cgcggcgaag tcctcgcccg agcgtccgag ggatccggtc tcggccgtgc
     5641 cgtcggggtc cgggcccacg ctgcggggca ggggcgggat gtcgtccacc cggggcagga
     5701 cggtgacctc cacggcgggc gcggcggggt tgcgggtgct ggcgaggccg aaggggtcgg
     5761 acatggccac ctgcatcggg ccgaccgtga cgaggccgcg cttggtcgtg ggaagtcggt
     5821 aggcgacatc ggtcgtgcgc cgcaggccga tgggggcgag gagcaggtcg gcgccggccg
     5881 tgcccgtcac cgggtcgagc aggcgcagga ccggggtggc acggccggcc cggttgcgca
     5941 cctggatcgc caccgtgcag gggtttccga cgtgcacccg cgacgggcgc acgctgcggg
     6001 tcacgacgat gtcgaggcgc cggagcccga cccagaggcc gcacaccagg gcgagcagcc
     6061 cgaccgcagc gccgatggca tagagctcgg cgaggccgag caggcggccg gccacgacca
     6121 gcaccgcgct ggcgatgccg accagcaacc cggtgcgggt ggggcgcacg gctcgtcagg
     6181 ccgaccgggg tcgggggacg gggacgctgt cgagcacgtc ggccaccacg tcggccgcgg
     6241 tgacgccctg caggtcggcc tccggggtga cgagcaggcg gtgggc
//
LOCUS       DATISU010000418         7157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_36829, whole genome shotgun sequence.
ACCESSION   DATISU010000418 DATISU010000000
VERSION     DATISU010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36829"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(43..141)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            189..1163
                     /locus_tag="VLR27_16560"
     CDS             189..1163
                     /locus_tag="VLR27_16560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP05121.1"
                     /translation="MSAVTGSAVRIEADQLDLVARAWDAAVDRTEGADEFCASSLWSF
                     AAAASFPDHDPPVVVGDGSSFAGMRRATAEDGSRLLLGLDPVWGFATPMVGHPVQAAR
                     LLGARLGLDDHDVAVVTGQRHGGIGLQCLVQVLGERRRLLQGPTEQRLQADLSDGLEP
                     WLARRSSRFRQRLRQVRRRAEEAGVTVVDCSALAPDDAVDRVLAVEARSWKGEEGTGL
                     ASESLADFYRRMAWRLAASDQLRLLFARQGDRDIGYVLGGVRGRTYRGLQLSHAADAR
                     DLGIGHLLQIHQLEALVGIDVYDLGMDMPYKRRWADRIDETFAVLIAP"
     gene            complement(1194..1793)
                     /locus_tag="VLR27_16565"
     CDS             complement(1194..1793)
                     /locus_tag="VLR27_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05122.1"
                     /translation="MPVDPDHRVVTATIIGVPAPGTVVWRADGERTRDGSPVTGDLDD
                     ADEVLRAGDQVVLEVVRDHFRWLVVDVVDRIPRPRTPARPRRPHAHPARPPGTVLIAW
                     LPFTRDDDEGPGKHRPCVVLRSTDPAVIRARPLYDPGSAVARSSGGVQLQAWRAAGLD
                     KASVAVDPVEIPVARCAQTLGHLSPVDLAGLGITGRRQR"
     gene            complement(1806..1970)
                     /locus_tag="VLR27_16570"
     CDS             complement(1806..1970)
                     /locus_tag="VLR27_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05123.1"
                     /translation="MGLFRRKGKDDFKALSKQRRVVEDEQPWFARDDDGPELDVETGI
                     GSNLRRDDAD"
     gene            2016..2372
                     /locus_tag="VLR27_16575"
     CDS             2016..2372
                     /locus_tag="VLR27_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05124.1"
                     /translation="MGAETVERRCRWCARRFTVTVGPGRPREFCRRSCRQRDYEARQR
                     ASEVGLSEHELVLTRQAMDDLRDRLYVLECAVEDVERDLGGSPTRAEYQEALDWLLDA
                     ARPVVESLGREGRTTD"
     gene            2470..2871
                     /locus_tag="VLR27_16580"
     CDS             2470..2871
                     /locus_tag="VLR27_16580"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HSP05125.1"
                     /translation="MVRITDGGPPMAARIDEKHRRRLRQERKELTDMLEALQGQLRSL
                     VDEGREVAESDFSEEGGDPDGGVVERDRVRARIADTEEHLRQVDRAEQAIEDGTYGTC
                     RVCGNEVPEERLEALPWTTLCVQCKAAGHAA"
     gene            3242..3886
                     /locus_tag="VLR27_16585"
     CDS             3242..3886
                     /locus_tag="VLR27_16585"
                     /inference="COORDINATES: protein motif:HMM:NF039596.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP05126.1"
                     /translation="MAPRSRTGSRPSREERRAQLLEAAVDAIRTIGPNASMEQLAAQG
                     GVTKPILYRHFGDREGLGRAIGEHYATVLMTRLATPLESQAPRDLLWRTVDTYLEFLE
                     DEPQLYTFLTSGGGVRPDLEDAGAIVEAVAREVAVRIGDQLRAQGLDAGGAEPFAHGI
                     VGMVHQAGDWWVRNRTMSRQALTTYLPTLLWSGFEGIARGAGADPAVISGTHVD"
     gene            3919..4692
                     /locus_tag="VLR27_16590"
     CDS             3919..4692
                     /locus_tag="VLR27_16590"
                     /inference="COORDINATES: protein motif:HMM:NF004855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HSP05127.1"
                     /translation="MPATVPHPTPHKLPEFASVEEERLHRKQRLAATFRLFGRFGFNE
                     GVAGHVTARDPEHPELFWVNPFGLSFDLVRVSDLILVDHNGVVVEGDWPVNQAAFAIH
                     SQIHAARPDAVAAAHTHSKAGRAWSTLARPLDMITQDACAFYNDHAVFDDYTGVVLDV
                     EEGKRIAHALGDNKAAILRNHGLLTVGGSVDEAAWWYISMERSCEVQLMAEAAGTPIL
                     IDEDGAKQAHSQVGSAFAGWFSFQPLYDKILRDQPDLLD"
     gene            4697..5125
                     /locus_tag="VLR27_16595"
     CDS             4697..5125
                     /locus_tag="VLR27_16595"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HSP05128.1"
                     /translation="MADVVPISDKLRDAREQVLRRHLEAHCAGDIDAVVATFARPRME
                     LIASGRVLDGAEALRAYLAERRKAFPDQSFEVICHHHSDRAVVSEHWMTGTHLGEVHG
                     VEPTGKRFRARMASIFDFEGEDLVNQRLYYDAGTIARQLA"
     gene            complement(5154..>7157)
                     /gene="glgX"
                     /locus_tag="VLR27_16600"
     CDS             complement(5154..>7157)
                     /gene="glgX"
                     /locus_tag="VLR27_16600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HSP05129.1"
                     /translation="RVELCLFSRDGRETRHELVEVDAHCWHAYLPGVGPGQQYGFRVH
                     GPWAPADGHRGNPHKLLLDPYAKAVTGTVDWDEACFSHRFDDPTRPNVEDSAPHVPKS
                     VVVNPFFHWGNDHAPGTPLEDTIIYEVHVKGFTQTMPGIPDELRGTYAGMAHPAAIQH
                     LRRLGVTAVELLPTHQFIHDHWLVEKGLTNYWGYNSIGFLAPHNGYAAWGDEGGQVQE
                     FRHMVKSMHDAGIEVILDVVYNHTAEGDHDGPTLSFKGIDNAAYYRLEDADRARYRDY
                     TGTGNSMNMRHPHVLQLIMDSLRYWVTEMHVDGFRFDLAATLARELHEVDRLSAFFDL
                     IQQDPIVSQVKLIAEPWDVGEGGYQVGNFPPLWSEWNGKFRDTVRDYWLQQHIPALRD
                     FGYRFTGSSDLYEASSRRPSASVNFVTAHDGFTLADLVAYERKHNEANGEDNRDGTDD
                     NRSSNHGVEGPTDDPEIQAVRRRQRKNLLITVLCSQGVPMLLGGDELGRTQRGNNNAY
                     CQDSELSWFDWSDVDHEMLDFTRRLVDLRREHPVFRRRRWFQGRPIHGSDVFDIAWLK
                     PDGTSMTHDDWNSGLADAVGIFLNGQAMPSVDRRGEPIVGESFLLLLNPTAESIEWTL
                     PGEPLGERWLVELSSDELLSSQLVRGRSMGVCARSATVMRRLG"
BASE COUNT          990 a         2581 c         2549 g         1037 t
ORIGIN      
        1 cttgctcctt cggtgagccc gcaggctgct ctccagaggt gcctcgtccc ggcggtccgg
       61 tggcctgaga ggttccgggg cggttgctcc ttcggcgccc tccccactgg cggtggggcg
      121 ggactctccc gtcgggggtc gacagcaggg ccatcgtaga cccgcatccc gcccctactg
      181 tgcacgccgt gtccgccgtg accggctccg ccgtgcgcat cgaggccgac cagctcgacc
      241 tggtggcccg cgcctgggac gccgccgtcg accgcaccga gggcgcagac gagttctgcg
      301 catcgagcct ctggtccttc gccgccgcgg cgtcgttccc cgatcacgat ccgccggtgg
      361 tcgtcggcga cgggtcctcc ttcgccggca tgcgccgggc gaccgccgag gacggcagcc
      421 ggctgctcct cggtctggac ccggtgtggg gcttcgccac tccgatggtc ggccaccccg
      481 tgcaggcggc ccggctcctc ggcgcgcgcc tcggcctcga cgaccacgac gtcgccgtgg
      541 tcaccggcca gcgccacgga gggatcggcc tgcagtgcct cgtgcaggtg ctgggggagc
      601 gtcgccggtt gctgcagggc cccacggagc agcggctgca ggccgacctg tccgatgggc
      661 tcgagccgtg gttggcccgc cgctccagcc ggttccgcca acggttgcgc caggtccgcc
      721 ggcgcgctga ggaggcgggc gtgaccgtcg tcgactgctc cgccctcgcg cccgacgacg
      781 ccgtcgaccg ggtgctcgcc gtggaggcac gcagctggaa gggcgaggag gggaccggcc
      841 tggccagcga gagcctggcc gacttctacc ggcgcatggc ctggcgcctc gccgcctccg
      901 accagctgcg cctgctgttc gcccgccagg gcgaccgcga catcggctac gtgctcggcg
      961 gtgtgcgggg ccgcacgtac cgcggcctgc agctgtccca cgccgccgac gcccgggacc
     1021 tcggcatcgg gcaccttctc cagatccacc agctcgaggc cctcgtcggc atcgacgtct
     1081 acgacctcgg catggacatg ccctacaagc gccgctgggc cgaccgcatc gacgagacct
     1141 tcgccgtcct catcgccccc tgaccctgtg aactggcagt gctggtgttg cggtcagcgc
     1201 tgccggcgac ccgtgatgcc gaggccggcg aggtcgacag gggagaggtg cccgagggtc
     1261 tgcgcgcacc gagcgaccgg gatctccacc gggtcgaccg ccacgctcgc cttgtccagg
     1321 ccggcagcac gccacgcctg gagctgcacg ccacccgagc tgcgggccac ggcgctgccg
     1381 gggtcgtaga gcggacgggc ccggatcacc gctggatcgg tcgagcgcag caccacgcag
     1441 ggccggtgct tgcccgggcc ctcgtcgtcg tcgcgggtga agggcagcca ggcgatgagc
     1501 accgtccccg gcggccgcgc cgggtgggcg tgggggcgcc ggggtcgggc gggggtccga
     1561 gggcggggga tgcgatcgac cacgtcgacc accagccagc ggaagtggtc gcggaccacc
     1621 tccagcacca cctgatcgcc ggcccggagc acctcgtcgg cgtcgtcgag gtcgccggtg
     1681 acgggggagc cgtcccgggt ccgctcgccg tcggcccgcc acacgaccgt gcccggggcg
     1741 gggacgccga tgatcgtggc ggtgacgacg cggtggtccg ggtcgaccgg cacggggtgg
     1801 ggtgactagt ccgcgtcgtc gcggcgcagg ttcgagccga tgccggtctc gacatcgagc
     1861 tcgggaccgt cgtcgtcccg ggcgaaccac ggctgctcgt cctcgaccac ccggcgctgc
     1921 ttcgagagcg ccttgaagtc gtccttgccc ttcctgcgga acagacccat ggcgagatcc
     1981 tgccaggtcg ctccggcgat cggtaccgtc gagtcgtggg tgccgagacc gtcgagcgac
     2041 gctgccgctg gtgcgcccgg cgcttcacgg tcaccgtcgg accgggccgg ccccgcgagt
     2101 tctgtcgccg gtcgtgccgg cagcgcgact acgaggcccg ccagcgggcg agcgaggtgg
     2161 ggctgtccga gcacgagctg gtgctgaccc gccaggccat ggacgacctg cgcgaccggc
     2221 tctacgtgct ggagtgcgcg gtggaggacg tcgagcgcga cctcggtggc tcgccgaccc
     2281 gggccgagta ccaggaggcg ctcgactggc tgctggacgc agcgcggccg gtggtcgagt
     2341 ccctgggacg cgagggcagg acgacggact agtcactcgt tctgtcaccg tgacgaaatg
     2401 accacacccg tggggcgtac gtctggaagg ggtgcgcatg tgggaaggtg cgccctgcac
     2461 catcagccga tggtgcggat caccgacgga ggaccaccca tggccgctcg catcgacgag
     2521 aagcaccgcc gccggctccg gcaggagcgc aaggagctga ccgacatgct cgaggcactg
     2581 cagggccagc tgcgcagcct cgtcgacgag gggcgtgagg tcgccgagtc ggacttctcc
     2641 gaggagggcg gcgaccccga cggcggggtg gtcgagcgcg accgggtccg ggcccgcatc
     2701 gccgacaccg aggagcacct gcgccaggtc gaccgggccg agcaggcgat cgaggacggc
     2761 acctacggca cctgccgggt gtgcggcaac gaggtgcccg aggagcgctt ggaggcgctg
     2821 ccctggacga cgctgtgcgt ccagtgcaag gccgccggcc acgctgcctg aggagggagg
     2881 aggggtcgtt tcgacgcccc tgcccctcca aggtcgcaac tttacagaac gtggattctc
     2941 ggcgaaggcc agggaagccg ggagtccgcc ggaccccgct ccacctgcgg gaccgtccct
     3001 gcgttctggc agttctcacg tcacgccagc gagcgacagc cgcccgacgc gctggcgggc
     3061 tgtcgagcgg ccacgacgga cgccgcccgg cgctacctcg tcactgccag tttccgtggg
     3121 gcgccgaccc cgggcgcgcc aacacccact tcccccggtt ccccgtcacc cgcccctcgt
     3181 ttcgtcactg tgacgttacg agttgctcag ggcgccggcg gcgcaccggt agcctgtggg
     3241 gatggccccg cgcagcagga ccggctcccg cccgagccgt gaggaacgac gagcccagct
     3301 gctcgaggcc gccgtcgacg ccatccgcac catcgggccg aacgcgtcca tggagcagct
     3361 ggctgcccag ggcggggtga ccaagccgat cctgtaccgc cacttcggcg accgcgaggg
     3421 tctgggacgc gccatcggcg agcactacgc caccgtgctc atgacccgcc tggcgacccc
     3481 gctggagagc caggcacccc gcgacctgct ctggcgcacc gtcgacacct acctcgagtt
     3541 cctcgaggac gagccccagc tctacacgtt cctgacctcg ggcggcggcg tgcgccccga
     3601 cctcgaggat gccggcgcca tcgtcgaggc ggtcgcccgc gaggtcgccg tccgcatcgg
     3661 tgaccagctg cgggcccagg ggctcgacgc cggcggggcc gagcccttcg cccacggcat
     3721 cgtcggcatg gtgcaccagg ccggcgactg gtgggtccgc aaccgcacca tgtcccgcca
     3781 ggccctcacc acctacctcc cgacgctgct gtggagcggc ttcgagggca tcgcccgggg
     3841 cgccggggcc gaccccgcag tgatctccgg cacacacgta gactgaggtc cctcgcgccg
     3901 ccgtcgaagg agacctccgt gcctgccacc gtgccccatc ccaccccgca caagctgccg
     3961 gagttcgcat ccgtcgagga ggagcgcctg caccgcaagc agcgcctcgc cgccacgttc
     4021 cgcctgttcg gccggttcgg cttcaacgag ggcgtcgccg gccacgtcac ggcccgtgac
     4081 cccgagcacc ccgagctgtt ctgggtgaac cccttcgggc tcagcttcga cctggtgcgg
     4141 gtgtccgacc tgatcctcgt ggatcacaac ggcgtcgtgg tcgagggcga ctggccggtc
     4201 aaccaggccg ccttcgccat ccactcccag atccacgctg cccgccccga cgcggtcgct
     4261 gccgcccaca cccactccaa ggccggccgg gcgtggtcga cgctggcccg cccgctggac
     4321 atgatcaccc aggacgcctg cgccttctac aacgaccacg ccgtcttcga cgactacacc
     4381 ggcgtcgtgc tcgacgtcga ggagggcaag cgcatcgccc acgccctcgg cgacaacaag
     4441 gcggcgatcc tgcgcaacca cggcctgctc accgtcggcg gctccgtcga cgaggcggcc
     4501 tggtggtaca tcagcatgga gcgcagctgc gaggtgcagc tcatggccga ggccgccggc
     4561 accccgatcc tcatcgatga ggacggcgcc aagcaggccc acagccaggt cggctccgcc
     4621 ttcgccggct ggttcagctt ccagccgctg tacgacaaga tcctccgcga ccagcccgac
     4681 ctgctggact gacgcggtgg ccgacgtcgt cccgatcagc gacaagctcc gggatgcccg
     4741 ggagcaggtc ctgcgccgcc atctcgaggc gcactgcgca ggcgacatcg acgcggtcgt
     4801 cgccaccttc gcccggcccc gcatggagct catcgcgtcc gggcgggtgc tcgacggcgc
     4861 cgaggcgctc cgggcctacc tcgccgagcg gcgcaaggcg ttccccgacc agtccttcga
     4921 ggtcatctgc caccaccaca gcgaccgtgc cgtggtgtcg gagcactgga tgaccggcac
     4981 gcacctcggt gaggtccacg gcgtcgagcc caccggcaag cggttccgcg cccggatggc
     5041 gtcgatcttc gacttcgagg gcgaggacct ggtcaaccag cgcctctact acgacgccgg
     5101 caccatcgcc cgccagctgg cctagatccg acgcaggccc cctgcgggcg tgcctagccc
     5161 aaccggcgca tgacggtggc cgagcgggcg cacacgccca tcgagcggcc gcgcaccagc
     5221 tggctggaga ggagctcgtc ggacgacagc tccacgagcc accgctcccc cagcggctca
     5281 ccgggcagcg tccactcgat cgactcggcg gtcgggttga gcagcagcag gaagctctcg
     5341 ccgacgatgg gctcgcctcg ccggtcgacc gacggcatcg cctggccgtt gaggaagatg
     5401 ccgacggcgt cggccaggcc cgagttccag tcgtcgtgcg tcatcgacgt gccgtccggc
     5461 ttcagccacg cgatgtcgaa cacgtcggac ccgtggatcg gacgaccctg gaaccagcgg
     5521 cgccggcgga acaccgggtg ctcccggcgc aggtccacga ggcggcgggt gaagtcgagc
     5581 atctcgtggt ccacgtcgga ccagtcgaac cacgacagct cgctgtcctg gcagtaggcg
     5641 ttgttgttgc cccgttgggt gcggccgagc tcgtcaccgc cgagcagcat gggcacgccc
     5701 tgggagcaca gcacggtgat cagcaggttc ttgcgctgcc gccgtcgcac cgcctggatc
     5761 tccggatcgt cggtgggacc ctcgacgccg tggttcgacg agcggttgtc gtcggtgccg
     5821 tcccggttgt cctcgccgtt ggcctcgttg tgcttgcgct cgtaggcgac gaggtcggcg
     5881 agggtgaagc cgtcgtgggc ggtcacgaag ttcaccgatg ccgagggcct gcggctcgat
     5941 gcctcgtaga ggtccgacga gccggtgaag cggtagccga agtcgcgcag ggcggggatg
     6001 tgctgctgga gccagtagtc gcgcaccgtg tcgcggaact tgccgttcca ctcggaccac
     6061 agcggcggga agttgccgac ctggtagccc ccctccccca cgtcccacgg ctcggcgatc
     6121 agcttcacct gcgacacgat cgggtcctgc tggatgaggt cgaagaacgc agacaggcga
     6181 tcgacctcgt gcagctcccg ggccagggtg gcggccaggt cgaagcggaa cccgtcgacg
     6241 tgcatctcgg tgacccagta gcgcagcgag tccatgatga gctggagcac gtgggggtgg
     6301 cgcatgttca tcgagttccc ggtgccggtg tagtcccggt agcgggcccg gtcggcgtcc
     6361 tcgaggcggt agtacgcggc gttgtcgatc cccttgaacg acagcgtcgg cccgtcgtgg
     6421 tcgccctcgg cggtgtggtt gtagaccacg tcgaggatca cctcgatgcc ggcgtcgtgc
     6481 atcgacttga ccatgtggcg gaactcctgg acctgcccgc cctcgtcgcc ccacgccgcg
     6541 tagccgttgt ggggcgccag gaagccgatc gagttgtacc cccagtagtt ggtgaggccc
     6601 ttctcgacca gccagtggtc gtggatgaac tggtgcgtcg ggagcagctc gaccgcggtg
     6661 acccccaggc ggcggaggtg ctggatggcc gccggatggg ccatgccggc gtaggtgccg
     6721 cggagctcgt cggggatgcc cggcatcgtc tgggtgaagc ccttgacgtg gacctcgtag
     6781 atgatcgtgt cctcgagcgg ggtgcccggg gcgtggtcgt tgccccagtg gaagaacggg
     6841 ttgaccacca ccgacttggg gacgtggggg gcgctgtcct ccacattggg ccgggtcggg
     6901 tcgtcgaagc ggtgggagaa gcaggcttcg tcccagtcga cggtgccggt gacggccttg
     6961 gcgtacgggt cgagcagcag cttgtgcggg ttgccgcggt gtccgtccgc cggtgcccac
     7021 gggccgtgga cccggaagcc gtactgctga cccgggccga ccccgggcag gtaggcgtgc
     7081 cagcagtgcg cgtcgacctc gaccagctcg tggcgggtct cacggccgtc gcggctgaac
     7141 aggcacagct cgacgcg
//
LOCUS       DATISU010000419         7939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_501873, whole genome shotgun sequence.
ACCESSION   DATISU010000419 DATISU010000000
VERSION     DATISU010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501873"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="VLR27_16605"
     CDS             complement(<1..1269)
                     /locus_tag="VLR27_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HSP05130.1"
                     /translation="MTGSASFARSEHTAAMARARLDEVAASAAAAEAAQRDLLAGVVE
                     LWPTSAWLAAGATSPKRWLLAYTHCSEQEAHRLERLAGLCHRHPALAEAALSGEVSMA
                     RAHTLGSAANPDREPWLADSLDAFLRLAERGCDDAAWAGAVRHWASLVDQERDVRRVP
                     AHSVVLSQKLFGGGEIHGDLSPNAFLNVATALDAWTDDPDPQDAPYQRSLGERRAGAL
                     DDICRAGLDPDSTRWGSADAHEEDEWEDVEAADTFDGFCPTDDLDEHLASADDIDPLV
                     VLRRRLRKAQAHRRRRARRRTRSRSGVCVNVHIDLRTLADLRDVDDLEDLVLRGDGWN
                     LTRAAADQLLCDAALTAVLFDGKGTLLDANDAAEQWSRKQRRAIAARDGGCVFPGCGR
                     PPRHCDIHHLHHRSRGGPTRTGNGAMLCRFH"
     gene            1318..1854
                     /locus_tag="VLR27_16610"
     CDS             1318..1854
                     /locus_tag="VLR27_16610"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HSP05131.1"
                     /translation="MDVARLEKVELVGEKWGGGPHYAGLVYVLGDDRHGTWLWGPAGR
                     TVSRGGVPVFRSEQDLIAVAPTGEWWMATWWVGHPEVELYVNINTPVERSAAAIRYVD
                     LDLDVVRLVDGACDIVDRDEFELHQVDLGYPPEITSAAATAADEVLSAVASGAPPFDG
                     SAARRWTDAARAARLPAV"
     gene            1982..2986
                     /gene="pheS"
                     /locus_tag="VLR27_16615"
     CDS             1982..2986
                     /gene="pheS"
                     /locus_tag="VLR27_16615"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HSP05132.1"
                     /translation="MTMMDELARIADEARARIAEAPTVETLDEVVRTTLGKKGSLKGL
                     KRELGRLDPDERKSVGQAVNDVIDELQASADDRRHDLERAERTQRLQADRLDLTEVMA
                     GSRAGHLHLVTQAIMELEDVFVGLGFTVAEGPEVETDHYNFGALNFPEGHPARDMYDT
                     LYVDFGEPGRTLLRTHTSPVQVRVMETTEPPIYSVMPGRTFRQDTADATHLPVFHQIE
                     GLVIDRGITLTDLAGTIDRFTKAYFGGEFSSRLRPSYFPFTEPSAEFDIIRPDGTFLE
                     LGGCGMVHPNVLRNCGIDPEEYSGFAFGFGIERLATTKAGVDDLRELITNDVRFLRQF
                     "
     gene            2997..5372
                     /gene="pheT"
                     /locus_tag="VLR27_16620"
     CDS             2997..5372
                     /gene="pheT"
                     /locus_tag="VLR27_16620"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HSP05133.1"
                     /translation="MKVLLSWLRDFAPIETDPVALGDVMSDLGMAVEEMDVLGEGLDG
                     IVVARVLDLRPHPDADKIQLVDVDAGDGEALQICCGAFNMSVGDLVPLATLGTTMPNG
                     MEIARRKLRGEWSNGMLCSPPEVNLPGDPSGILLLPEGLEPGVPLTEALGISADVLYD
                     LEINPNRPDAMSVAGVARDVAARLGLPFQLPSPSITATGPSTAELVSVEILAPERCGR
                     FTARVLQGVTVGPSDPLIANRLTLLGMRPINNVVDASNYLMLELGQPSHPYDLATLGG
                     AGFRVRVAQDGETITTLDDVERTVTPADLLICDATDVPVGIAGIMGGADSEISDATTD
                     VVVEMAWFERMPLAKSSRRLGVRSEASARFERGVDPDGIDLAQDRFVELLGGVGGGSV
                     GSVSDGLVDERGRLPDRTPIPVRPARVNAILGSDLSPQEMKDLLDPIGFTTELGADGE
                     PMRVALPSYRLDSEVEIDVVEEVGRMYGYSRLGKTMPVSSLSGHLTPRQLERRRIRSL
                     LTGLGLSEAAPMPFLAPGDLERAGLPGDGITITNPLVAEESVLRTSLLPGLLKAVRHN
                     VGHRNEGIALFEVGHVFRRPMDPDAELPDERETLAVIVAGADATAAVEAWRTLCDGLS
                     VPNADVANAEMPALHATRSGVVRVEGEPVGAIGEVDPEVLAAYDIPGRAAWLEVDLDR
                     FLAHPHGEPTYTPVSRFPSSDIDLAFEVAEGISATDVAATIERAAGALLGWVRLFDTY
                     RGEGVRDGHRSLTYRVRLQAHDRTLTDGEVGEVRQAIIDAVQSGFPAHLRG"
     gene            5420..5764
                     /locus_tag="VLR27_16625"
     CDS             5420..5764
                     /locus_tag="VLR27_16625"
                     /inference="COORDINATES: protein motif:HMM:NF042718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6614 family protein"
                     /protein_id="HSP05134.1"
                     /translation="MDVYEIQVDLRPGVRDTELVAALDTYLGGLAADGLIESWRLLRR
                     KLGLGAGGEFKVLIETRDLAQLDDAFRLVSSRSDPVEEAHHGVNSLVANFQAALYRDF
                     PDPHRRTGDERF"
     gene            complement(5772..7034)
                     /locus_tag="VLR27_16630"
     CDS             complement(5772..7034)
                     /locus_tag="VLR27_16630"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HSP05135.1"
                     /translation="MSGRMLGAAVALIVALAAGVAPVAAQSDPAGSGLVRPPVLVTGD
                     SLSHQAADDIARSLGALGYHDVRFAVFGGTTIGWSTDRVLEDASPIVVFASGTYNSLL
                     GWTAADQQEAQRAVQVLGQRRCAVWMLPAAARYPSGTPQAHAPSRDTVAGIRAAASGT
                     PVHLAEWDLVADALPEIHVPDGVHIDDGGQALYGDLIAGAVRHRCEDPDPARVAPWER
                     YASWTTTTLLGRLPAADELAVWTDRLAGGHPRLAFTRTLVTTPEWAGARIDDLYLRAL
                     GRTADPAGRTHWLQRLVAGTGFDVVAAHVYSSEEFVRGSGASSRDFVTALYRRLLQRE
                     PDGPGLDAWTRALEAGTSPMAVAVAFHRSPEARGGRVDELYRQILGRDPDPGGRATWI
                     GALARMDDLRLAARLAASGEAFRRAQDG"
     gene            complement(7034..>7939)
                     /locus_tag="VLR27_16635"
     CDS             complement(7034..>7939)
                     /locus_tag="VLR27_16635"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSP05136.1"
                     /translation="EVIVTDQDHEANRGAIERAATRAGATVRVWGSDPDTGLLDPAEL
                     EDLLSAATRLVHLPHASTIVGVLNDVHTLVRLVHEASAWAVVDGVSAVPHGIPDVGHL
                     HADVYLCSLYKVYGVHQGLMTVRRPLLDALPNQGHFFNADVVSKRLNPAGPDHPQVAC
                     SAAVLDMVLEQAEHHGEPEDLRGACDAMAARWQAHETAVATPLLAWLDERDDVRLLGP
                     VDDSAGHRCPTIAFVPEHQEPLAVATALADREIAVGSGHFYAWRTLEAMGVDPRRGVV
                     RASLVHYTSADDVERLIAGLDAVLD"
BASE COUNT         1059 a         2927 c         2868 g         1085 t
ORIGIN      
        1 gtggaagcgg cagagcatcg cgccgttgcc ggtgcgggtc gggccgcccc ggctgcggtg
       61 gtgcaggtgg tggatgtcgc agtgccgtgg tggacgaccg cagccgggga acacgcagcc
      121 gccgtctcgt gccgcgatgg cccgacgctg cttgcgggac cactgctcgg ctgcgtcgtt
      181 ggcatcgagg agcgtgccct tgccgtcgaa gagcacggcg gtcagcgcgg cgtcgcagag
      241 gagctggtcg gcagcggcac gggtcaggtt ccagccgtcg ccccgcagca cgaggtcctc
      301 gaggtcgtcg acatcgcgga ggtcggccag cgttcgcagg tcgatgtgga cgttgacgca
      361 gacccccgag cgggaccgag tccgccggcg ggcccggcga cgacggtggg cctgggcctt
      421 gcggagccgc cggcgcagca ccacgagcgg atcgatgtcg tccgcggagg cgaggtgctc
      481 gtcgaggtcg tcggtcgggc agaacccgtc gaaggtgtct gccgcctcca cgtcctccca
      541 ctcgtcttcc tcgtgggcgt cggcggagcc ccagcgggtg ctgtccggat cgaggccggc
      601 ccggcagatg tcgtcgaggg cgccggcccg gcgctcgccg agggagcgct ggtacggggc
      661 gtcctgcggg tcggggtcgt cggtccaggc gtcgagcgcg gtggcgacgt tcaggaacgc
      721 gttgggggag aggtcgccgt ggatctcgcc gccaccgaac agtttctgag agagcaccac
      781 ggagtgggcc ggcacccggc ggacgtcgcg ctcctggtcg acgagggatg cccagtggcg
      841 caccgcgccg gcccaggcgg cgtcgtcgca gccccgctcg gcgagccgga ggaacgcgtc
      901 cagcgagtcg gccagccacg gctcccgatc ggggttcgcg gcgctgccca gcgtgtgcgc
      961 tcgggccatc gacacctcgc cggacagggc cgcctcggcg agggcggggt ggcggtggca
     1021 gagcccggcg agtcgctcga gccgatgtgc ttcctgctcg gagcagtggg tgtaggcgag
     1081 cagccaccgc ttcggggatg tcgccccggc ggccagccag gccgaggtgg gccacagctc
     1141 gaccacgccg gccaacaggt ctcgctgcgc cgcttccgcg gccgccgccg acgcggccac
     1201 ctcgtccagg cgggcccgcg ccatcgcagc ggtgtgctcg ctgcgggcga aggaggcgga
     1261 accggtcatg tcacccattc tcctcagggg gtctgacagg aatggaagaa tgtgggcatg
     1321 gacgtcgctc ggctcgagaa ggtcgagctg gtcggtgaga agtggggcgg cggaccgcac
     1381 tacgccggcc tcgtctacgt cctgggcgac gatcggcacg gcacctggct ctggggtccg
     1441 gcggggcgca cggtgagtcg cggtggggtc ccggtgttcc gatccgagca ggatctgatc
     1501 gccgtcgcgc ccaccggcga gtggtggatg gcgacgtggt gggtcggcca cccggaggtg
     1561 gagctgtacg tgaacatcaa cacgcccgtg gagcggtcag ccgccgcgat ccgctacgtg
     1621 gacctcgacc tcgacgtggt gcgtctcgtg gacggcgcat gcgacatcgt cgaccgtgat
     1681 gagttcgagc tccaccaggt cgacctcggc tatccgcccg agatcacctc ggccgcagcg
     1741 acggctgccg acgaggtcct gtccgccgtg gcgtcgggcg ccccgccctt cgacggcagc
     1801 gcagcgcgcc ggtggaccga tgcagcgcgt gccgcgcggc tgcccgcggt ctgaacgaca
     1861 tccccgcacc agcagctgtt ccgtggtcgc tgccagtttc ccagcgcgcc cctgggactc
     1921 cgctccgccc gcgagcggct gaagcccttt gccagccgcc gccccggtcg ggcagggttc
     1981 catgaccatg atggatgagc tcgcccgtat cgccgacgag gcacgcgccc gcatcgccga
     2041 ggcgcccacc gtcgagaccc tcgacgaggt cgtgcgcacc acgctgggca agaagggctc
     2101 cctgaagggg ctcaagcgcg agctcggtcg cctcgacccc gacgagcgca agtcggtggg
     2161 ccaggccgtc aacgacgtca tcgacgagct gcaggcgtcc gccgacgatc gccgccacga
     2221 cctcgagcgg gccgagcgga cccagcggct gcaggccgac cgcctcgacc tcaccgaggt
     2281 gatggccggc tcccgggccg ggcacctcca cctcgtcacc caggcgatca tggagctcga
     2341 ggacgtcttc gtcggcctcg gcttcaccgt cgccgagggt cccgaggtcg agaccgacca
     2401 ctacaacttc ggggcgctca acttccccga gggccacccg gcccgggaca tgtacgacac
     2461 gctctacgtg gacttcggcg agcccggccg cacgctgctg cgcacccaca cctcgccggt
     2521 gcaggtgcgg gtgatggaga ccaccgagcc gccgatctac tcggtgatgc cgggccgcac
     2581 gttccgccag gacaccgcgg atgccaccca cctgccggtg ttccaccaga tcgagggcct
     2641 ggtcatcgac cgaggcatca cgctcacgga cctggccggc accatcgacc ggttcaccaa
     2701 ggcctacttc ggcggggagt tctcctcgcg cctgcgcccg tcgtacttcc ccttcaccga
     2761 gccctcggcc gagttcgaca tcatccgccc cgacggcacg ttcctcgagc tcggtggctg
     2821 cggcatggtg caccccaacg tgctgcgcaa ctgcggcatc gaccccgagg agtactccgg
     2881 gttcgccttc ggcttcggca tcgagcgcct ggccaccacc aaggcggggg tcgacgacct
     2941 gcgcgaactg atcaccaacg acgtccgctt cctgcggcag ttctaggagc ggcaccgtga
     3001 aggtccttct gtcctggctg cgcgacttcg cgccgatcga gaccgacccc gtcgcgctcg
     3061 gcgacgtcat gagcgacctc ggcatggccg tcgaggagat ggacgtcctc ggggagggcc
     3121 tcgacggcat cgtcgtcgcc cgggtgctcg acctgcgccc ccatcccgac gccgacaaga
     3181 tccaactggt cgacgtggac gccggcgacg gcgaggcgct gcagatctgc tgcggcgcgt
     3241 tcaacatgtc cgtcggcgac ctcgtcccgc tcgccacgct cggcaccacg atgccgaacg
     3301 gcatggagat cgcccggcgc aagctccgtg gggagtggtc caacggcatg ctctgcagcc
     3361 cgcccgaggt caacctgccc ggcgacccct ccggcatcct gctgctgccc gaaggcctcg
     3421 agccgggcgt gccgctcacc gaggccctcg ggatctcggc cgacgtgctc tacgacctcg
     3481 agatcaaccc gaaccgaccc gatgccatgt cggtggccgg cgtcgcgcgc gacgtcgccg
     3541 cccgcctggg gctgcccttc cagctgccct cgccgagcat caccgcgacc ggcccgtcca
     3601 ccgccgagct cgtctcggtc gagatcctcg cccccgagcg gtgcggccgc ttcacggccc
     3661 gggtcctgca gggcgtcacc gtcggaccct ccgatccgct catcgccaac cggctgacgc
     3721 tgctcggcat gcggccgatc aacaacgtcg tcgacgcctc gaactacctg atgctcgagc
     3781 tcggccagcc ctcccacccc tacgacctcg ccaccctcgg cggtgccggg ttccgagtgc
     3841 gggtggcgca ggacggggag accatcacca ccctcgacga cgtcgagcgc acggtcacgc
     3901 cggccgacct gctgatctgc gacgccaccg acgtgcccgt ggggatcgcc gggatcatgg
     3961 gcggcgccga ctcggagatc tccgatgcca ccaccgacgt cgtcgtggag atggcgtggt
     4021 tcgagcggat gccgctggcc aagtccagcc gccgtctcgg cgtgcgctcc gaggcctcgg
     4081 cccgcttcga gcgcggcgtg gatcccgacg gcatcgacct ggcccaggac cgcttcgtcg
     4141 agctgctcgg cggggtcggc ggcggctccg tcggttcggt gtccgacggc ctcgtcgacg
     4201 agcggggccg gctgcccgac cgcacgccca tcccggtgcg accggcgcgg gtgaacgcca
     4261 tcctcggctc cgacctgtcg ccgcaggaga tgaaggacct cttggatccg atcgggttca
     4321 ccaccgagct cggcgccgac ggggagccga tgcgggtggc gctcccgtcg taccggctcg
     4381 actccgaggt ggagatcgac gtcgtcgaag aggtcggccg catgtacggc tactcccgcc
     4441 tgggcaagac catgccggtc tcgtcgctgt cgggccacct caccccccgc cagctcgagc
     4501 gccgccgcat ccggagcctg ctcaccggcc tgggcctctc cgaggctgcg ccgatgccgt
     4561 tcctcgcccc tggcgacctc gagcgtgccg gcctcccggg cgacggcatc acgatcacca
     4621 acccgttggt cgccgaggag tccgtgctgc gcacgtcgct gctgcccggc ctgctcaagg
     4681 ccgtgcgcca caacgtcggc caccgcaacg agggcatcgc cctgttcgag gtcggccacg
     4741 tgttccgccg cccgatggac cccgatgccg agctccccga cgagcgtgag acgctcgcgg
     4801 tcatcgtcgc gggagccgac gcgaccgcgg ccgtggaggc gtggcgcacc ctgtgcgacg
     4861 gcctgtccgt gccgaacgcc gatgtcgcca acgccgagat gccggcgctg cacgccaccc
     4921 gctccggcgt ggtgcgggtc gagggcgaac cggtcggggc gatcggtgag gtcgaccccg
     4981 aggtgctcgc cgcctacgac atccccggcc gtgccgcctg gctcgaggtc gacctcgacc
     5041 gcttcctcgc ccacccccac ggcgagccca catacacgcc ggtgagccgg ttcccgtcct
     5101 ccgacatcga cctcgcgttc gaggtcgccg agggcatctc ggccaccgac gtcgccgcca
     5161 ccatcgagcg cgccgccggc gccctgctcg gctgggtccg cctgttcgac acctaccggg
     5221 gcgagggcgt ccgggacggc caccgctcgc tgacctaccg ggtccgcctg caggcccacg
     5281 accgcaccct caccgacggc gaggtcggcg aggtccgcca ggccatcatc gacgcagtcc
     5341 agtcgggctt ccccgcccac ctgcgcgggt agcgggaacc ggcgcgtccg cagcccgggg
     5401 gctgtgccag cctgccgccg tggacgtcta cgagatccag gtcgacctcc gtccgggcgt
     5461 gcgcgacacc gagctcgtcg ccgccctcga cacctacctc ggcggcctcg ccgccgacgg
     5521 cctcatcgag tcctggcgcc tgctgcgccg caagctcggg ctcggcgccg gtggcgagtt
     5581 caaggtgctc atcgagaccc gggacctggc ccagctcgac gacgccttcc ggctggtgtc
     5641 gagccgctcg gacccggtgg aggaggccca ccacggggtc aactccctcg tggccaactt
     5701 ccaggccgcg ctgtaccggg acttccccga cccgcaccgg cggaccgggg acgagcggtt
     5761 ctgatcggca ctcagccgtc ctgggcgcgg cggaacgcct caccggacgc ggcgagccgc
     5821 gccgcgaggc gtaggtcgtc catgcgagcc agcgcgccga tccacgtggc cctcccgccc
     5881 ggatccgggt cacgcccgag gatctgccgg tagagctcgt cgacccggcc gccccgggcc
     5941 tccggtgagc ggtggaacgc gactgcgacg gccatgggcg acgtgcccgc ctccagcgcc
     6001 cgggtccacg cgtccaggcc cggtccgtcg ggctcccgct ggaggagccg ccggtacagg
     6061 gccgtgacga agtcgcggct gctcgcgccc gacccgcgga cgaactcctc ggagctgtac
     6121 acgtgggcgg cgacgacgtc gaagcccgtg cctgcgacga ggcgctgcag ccagtgcgtg
     6181 cggccggccg ggtcggcggt gcgcccgagg gcgcggaggt acagatcgtc gatccgggcg
     6241 ccggcccact ccggggtggt caccagcgtg cgggtgaacg ccaggcgggg gtggccgccg
     6301 gcgaggcggt cggtccagac ggcgagctcg tcggccgccg gcagccggcc gagcaacgtc
     6361 gtggtcgtcc acgacgcgta gcgctcccac ggcgcgaccc tcgccgggtc cgggtcctcg
     6421 cagcggtgcc gaaccgcccc ggcgatgagg tcgccgtaga gggcctgccc accgtcgtcg
     6481 atgtgcacgc cgtccgggac gtggatctcg gggagggcgt cggcgacgag gtcccactcg
     6541 gccaggtgca ccggcgtccc gctggcggcg gcgcggatgc cggccacggt gtcccggctc
     6601 ggcgcgtgcg cctgcggggt cccggacggg tagcgggccg cggcggggag catccagacc
     6661 gcgcaccgcc gttgcccgag cacctggacg gcacgctggg cctcctgctg atcggctgcc
     6721 gtccagccca gcagcgagtt gtaggtcccg ctcgcgaaca cgacgatcgg tgaggcgtcc
     6781 tcgagcaccc ggtccgtgga ccagccgatc gtggtgccac cgaacacggc gaagcgcacg
     6841 tcgtggtacc cgagcgcgcc gagcgagcgg gcgatgtcgt ctgcggcctg gtgcgacagg
     6901 gagtcgcccg tgacgaggac cgggggacgc accagcccgg acccggccgg atcgctctgc
     6961 gctgcgacgg gggcgacgcc ggcggccagc gccacgatca gggcgacggc ggcgccgagc
     7021 atccggccgg acatcagtcg agcaccgcgt cgagcccggc gatcagccgc tcgacgtcgt
     7081 cggccgaggt gtagtgcacg agcgaggccc gcacgacccc tcgccgggga tcgacgccca
     7141 tggcctcgag cgtccgccac gcgtagaagt ggccggatcc gacggcgatc tcccgatcgg
     7201 ccagggcggt ggcgaccgcg aggggctcct ggtgctcggg gacgaaggcg atggtcgggc
     7261 agcggtgccc ggccgagtcg tccaccgggc cgaggaggcg gacgtcgtcg cgctcgtcga
     7321 gccaggccag caggggagtg gccaccgcgg tctcgtgggc ctgccaccgc gccgccatgg
     7381 cgtcgcacgc gccccggagg tcctccggct cgccgtggtg ctcggcctgc tcgaggacca
     7441 tgtccaggac cgccgccgag caggccacct gggggtggtc gggaccggcc gggttcaacc
     7501 gcttgctcac cacgtcggcg ttgaagaagt ggccctggtt cgggagcgcg tcgagcaggg
     7561 gccggcgcac ggtcatgagc ccctggtgca cgccgtagac cttgtagagg ctgcagaggt
     7621 agacgtcggc gtgcaggtgg ccgacgtcgg ggatgccgtg gggcacggcc gacaccccgt
     7681 ccacgacggc ccaggcactg gcctcgtgca ccaggcggac cagggtgtgg acgtcgttga
     7741 ggacgccgac gatggtcgag gcgtggggga ggtggaccag ccgggtcgct gccgacagca
     7801 ggtcctccag ctcggccgga tcgagcagcc cggtgtcggg gtcgctgccc cagacccgca
     7861 cggtggcacc cgctcgggtc gcggcgcgct cgatggcccc gcggttggcc tcgtggtcct
     7921 ggtcggtgac gatgacctc
//
LOCUS       DATISU010000420        10150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_316644, whole genome shotgun sequence.
ACCESSION   DATISU010000420 DATISU010000000
VERSION     DATISU010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 10150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10150
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316644"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="VLR27_16640"
     CDS             <1..1092
                     /locus_tag="VLR27_16640"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSP05137.1"
                     /translation="AIAERHQRDPKRITIKAGDVGTDSDLVRQTAYVVDRHHKAAALG
                     RILDVEAATSAIVFCRTRAEVDELTVTMNSRNHRAEALHGGMDQEQRDRVMGRLRDGT
                     AELLIATDVAARGLDVDTLTHVVNYDVPAAPEAYVHRIGRVGRAGRTGTAITLVEPRQ
                     RRLLENIERLTKQRLDLARIPSVDDLRATQIERTAEALEAVLVAGDLDEYGEVLEALD
                     EFDDRSIALAAIKLLREAEGSVDETEIPSASMKGPKGGKDKGKWDKGAKGGTKPTGRS
                     DRGRRTPDGPTGSVYVNLGSKAGVRPADLVGAIANEAGIPGREVGPIRIGDTYAVVGV
                     PEGAVEDVIKALNKGSVRGKPVKARRFVE"
     gene            complement(1103..1828)
                     /locus_tag="VLR27_16645"
     CDS             complement(1103..1828)
                     /locus_tag="VLR27_16645"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HSP05138.1"
                     /translation="MDLDFGDALRALLSTNLGRHDRLGFDLGERRHAAVAVVVVDSDA
                     DVHGADDERIDPALLADIPGAEDLTLTGSVAGTAGGPAVLLTRRASRMRAHAGQWALP
                     GGRLDAGETPIDAARRELQEELALDLPESAVLGVLDDYPTRSGYVITPVVLWGGADPT
                     MEPQPDEVASVHRIAFRELCRPDSPRFVTIPESERPVVQVPIGGDLIHAPTGAVLLQF
                     RRVAIEGGSERVADYEQPVFAWR"
     gene            complement(1852..3954)
                     /locus_tag="VLR27_16650"
     CDS             complement(1852..3954)
                     /locus_tag="VLR27_16650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HSP05139.1"
                     /translation="MALTAPPRPTTAEVEAELAQHVRALAGPDAVPRPEQVAAVQAVV
                     ADGRRTLLVARTGFGKSAVYFSATRMLRDRGWGPTVVVSPLLALMRDQVAAAQRLGLV
                     AETINSSNTDEWDRIEAAITADSVDLLLISPERLANQGFRERVFHLLLEQPMALVCDE
                     AHCISDWGHDFRPDYLRLRHLLDELPSWTPVLATTATANQRVTDDVASQLGSDALVLR
                     TTLDREALHLSVVDLPDDAHRLAWVSEHLDELPGSGIVYCLTQEQAAQTAEWLRLRGH
                     ACEAYTGGTEPEQRLVLEEQLRANELKALVATSALGMGFDKGDLAFCVHLGLPPTPVA
                     YYQQIGRAGRALERAEVVALPRPTEDAAIWRWFEQVSLPPESTCDEVLLQLSSSRPTS
                     VPTLESVVNLGRSRLQTLLKILEVDGAIRAVKGGYLLADEGWTYDREKAERLRRLRRD
                     ESEQMLAFADRPGCRLRFLREALDDAEAEDCGRCDRCLGTVRSTDLDPELVAEAGRHL
                     RAGDVGVEPRRQWPSGLDEPKGRIKPEAQARWGRALCRVGDGGWGPMIDEVLTGDRLL
                     HEDMIRAVAGVLKRWEWEQRPGWICPVPSRRRQGLIDRLCSGLGQLGKLPVHPALVRI
                     EDGGFQADQANSAHQVANVWGRFAVDPDQLPEGALLDGPVLLVDDECDSRWTLTVAAH
                     LLREAGTGPVLPLVLRSR"
     gene            complement(3965..5353)
                     /locus_tag="VLR27_16655"
     CDS             complement(3965..5353)
                     /locus_tag="VLR27_16655"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSP05140.1"
                     /translation="MVEVDHTSADGHAASSPSGDAEHDAFGDAFDLSPIGLAVVALDG
                     SLLRVNPTLCELIGYPASDLASRMFQELTHPDDLDGDVTLAREVLAGIRPTYSVDKRY
                     VHRDGSIVHTRQSVSLVRWADGRPRHFIAQIEDRTDAVAAQRMLEAVVEQMSDQIYVI
                     GPDGRYSWASPSAVALFGVDDFEGAAVGRFMHPDDRDAIVSEMAAALAGTAPIPERRT
                     FRVWSQALEEWRWLEATVSDRTDDPLLGGLLVCSRDVTQREADAAALRHQALHDDLTG
                     LPNRALLVDRVDGAARRAARSGTEIAVLFFDVDHFKQVNDTLGHAAGDRLLIEVAERV
                     RSRLRAQDTLGRLGGDEFVVVLEGLGEQPEAAVSAICDDLVDALAEPMDLDGRRVRMS
                     SSIGVVISDGTDDAEELLRAADEAMYATKASGRGRWLLHTEAMSTAARARFDSAEDEL
                     TETGPVTVRGSR"
     gene            complement(5416..7776)
                     /locus_tag="VLR27_16660"
     CDS             complement(5416..7776)
                     /locus_tag="VLR27_16660"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HSP05141.1"
                     /translation="MDPELTLGETIDPAERERTGTPLVVDASDLTTHGVIVGMTGSGK
                     TGLGVVLIEEALRAGIPTLVLDPKGDMTNLALRFPGLSGEEFAPWVAPGADAAETATT
                     WREGLAGWGLDGTHIAELDERSDVTVYTPGSTAGVAMDLVGSLSAPPDADEEAQADEI
                     DGLVSGLLSMVGITADPLSSREHILLSNLVQRAWTAGEDLDLGQLVHQVQDPPIRKLG
                     VLELESFYPAADRTALAMQLNGLLASPSFAAWSQGVPIDIGSMLSTDDGRAACSVIYL
                     AHLSEEERQFVVTLVLSKLVTWMRGQSGTPDLRALVYMDEVFGYVPPSAAPPAKKPIL
                     TILKQARAFGVGMVLSTQNPVDLDYKALSNAGTWMIGRLQTERDVDRLRDGLQAASGS
                     VDAEQVRTTIAGLGKREFVLHSTKASAPSVFTTRWALDYLAGPLSKDQVGALPGQATA
                     APPAATSTTSTSSTTPVEERPLEVGDDETPVPPAVADGVPVRWVDPAAPWLPEVGGDP
                     SSTRHRAGLAVRVSLLFDETKADLRHTEEFECVLVGLGEHLDPAELVTVDYDDRDLRE
                     AAPTPALYELPAAPIGKATWFRDAKAAVVDHLHAERTVTVLRNADLKLWSRPGESAED
                     FAVRCRAAGEAAAEVDAAKVRTQLERRMGTIRKAIDREQTRVAELEARAGDAKQHELV
                     SAAGDLLGSFLGGRRSARSIVSKVKGVSSRRKQTSAAGQRLETALAGVEAKTLELADL
                     EAELVDELAEIEAGWADVADQVEEVEVGLEKNDIDVRQLALIWVPA"
     gene            7845..8933
                     /locus_tag="VLR27_16665"
     CDS             7845..8933
                     /locus_tag="VLR27_16665"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HSP05142.1"
                     /translation="MPLFQVTPEEIRIACEVETAEEAGLVCRNVYTWTENDLLARVAD
                     WLATVPLRILIIFVIAWVVNRLVRKSIGSFVANMAADDHQEARSRLRKYTPRPLQSTT
                     AVQPLRAASRAQTIGLVLKSTASIVVYSIAVITALAEVGINLAPLIAGAGIVGVALGF
                     GAQSLVKDFLSGLFMLAEDQYGVGDVIDVGEAVGTVEVVNLRTTKLRGVDGTVWHVPN
                     GEIRRVGNMSQQWARALIDVEVAYDTDIGKAEAVVKEVADQLWRDDIWRTDVLEEPEI
                     WGVETLGASGVIIRLVVKTQPGRQFAVSRELNRRIKDRFDTEGIEIPFPQQVMWVRRE
                     AGSSTDSAGGPLEDGSEGAGDEAGAPSA"
     gene            8930..9883
                     /locus_tag="VLR27_16670"
     CDS             8930..9883
                     /locus_tag="VLR27_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSP05143.1"
                     /translation="MTSRPVRARALEATASLNPGAFSVGDWGLFVSIALIWGSSFLFI
                     AIALDSIEPGLITWGRVALGAAVLNVVPKARTRLDPEDRFRMVLLSLLWVGIPFTLFP
                     LAEQHISSAVTGMLNGSMPIFAALVAGVLLRTPPGPTQAAGLVVGLAGTLAVSLDQLG
                     EGDNAWIGVLMALGATVCYGFAINIAAPLQRRYGSLAVMARMLALATVWTAPFGLWSI
                     AGSTFELGPALSVAFIGAVGTGIAFVIMATLVGSVGSTRASMATYLIPVVAMVLGVLV
                     RDERVGVIAVVGVVLVVVGAYFASRPERAVPPAPIAIREPT"
     gene            complement(9926..>10150)
                     /locus_tag="VLR27_16675"
     CDS             complement(9926..>10150)
                     /locus_tag="VLR27_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05144.1"
                     /translation="PEHLFVVDPAVILTAVDRLERKGGREVMARCPTADDADAAAAWL
                     TDRVGRLAPNLGATAEVDEGGGQYLVSLRA"
BASE COUNT         1384 a         3594 c         3788 g         1384 t
ORIGIN      
        1 gccatcgccg agcgccacca gcgggacccg aagcgcatca cgatcaaggc cggcgacgtc
       61 ggcaccgact cggacctggt ccgccagacc gcctacgtcg tcgaccgcca ccacaaggct
      121 gccgccctcg gccgcatcct cgatgtggag gcggccacct cggccatcgt gttctgccgc
      181 acccgggccg aggtcgacga gctcaccgtc acgatgaaca gccgcaacca ccgggccgag
      241 gccctgcacg gcggcatgga ccaggagcag cgcgaccggg tcatgggtcg cctgcgcgac
      301 ggcaccgccg agctgctcat cgccaccgac gtcgcggccc gcggcctcga cgtcgacacc
      361 ctcacccacg tcgtcaacta cgacgtgccg gctgcgcccg aggcctacgt gcaccgcatc
      421 ggccgggtgg gccgcgccgg gcggaccggc accgccatca cgctggtcga gccacgccag
      481 cgacgcctgc tcgagaacat cgagcggctg accaagcagc gcctcgatct ggcccgcatc
      541 cccagcgtcg acgacctccg cgccacccag atcgagcgga ccgccgaggc gctggaggcc
      601 gtgctggtcg ccggcgacct cgacgagtac ggcgaggtgc tcgaggcact cgacgagttc
      661 gacgaccgca gcatcgccct tgccgccatc aagctcctcc gtgaagccga gggatcggtc
      721 gacgagaccg agatccccag cgcctccatg aaggggccca agggcggcaa ggacaagggc
      781 aagtgggaca agggtgccaa gggtgggacc aagccgaccg gccgcagcga ccggggccgg
      841 cgcacccccg acggcccgac cggatccgtg tacgtgaacc tcggctccaa ggccggtgtc
      901 cgtcccgccg acctggtggg tgccatcgcg aacgaggccg gcatccccgg tcgggaggtc
      961 gggccgatcc gcatcggcga cacctacgcc gtcgtcgggg tgcccgaggg tgcggtggag
     1021 gacgtcatca aggccctcaa caagggctcg gtgcggggca agccggtgaa ggcccgccgc
     1081 ttcgtcgagt agcgctcgct ggtcagcgcc aggcgaagac gggctgctcg tagtcggcga
     1141 cccgctcgct cccgccctcg atggcgacgc ggcggaactg cagcagcacg gccccggtgg
     1201 gcgcgtggat gaggtcgccc ccgattggca cctggaccac cgggcgctcc gattcgggga
     1261 tcgtgacgaa ccgcggcgag tccgggcggc acagctcgcg gaacgcgatc cggtggacgg
     1321 aggcgacctc gtcgggctgg ggctccatcg tcgggtcggc gccaccccag agcacgaccg
     1381 gcgtgatgac gtagcccgac cgggtcgggt agtcgtcgag gacgcccagc accgcggact
     1441 cgggcaggtc gagggcgagc tcctcctgca gctcccgacg ggcggcatcg atcggcgtct
     1501 cgccggcatc gagccggccg ccggggagcg cccactgccc ggcgtgggcc cgcatccggc
     1561 tggctcgtcg ggtgagcagc accgccggtc cgccggcggt cccggccacg ctgccggtga
     1621 gcgtcaggtc ctcggccccg gggatgtcgg cgagcagcgc cgggtcgatg cgctcgtcgt
     1681 cggccccgtg cacgtcggcg tcgctgtcca ccacgaccac cgccaccgcg gcgtggcgcc
     1741 gctcgccgag gtcgaagccg aggcggtcgt gccgtcccag attggtgctg aggagggcgc
     1801 ggagcgcgtc cccgaagtcg agatccacgg tgcgcaccgt atggccgggg ctcagcggct
     1861 gcgcagcacc aggggcagga ccgggccggt gccggcctcc cgcaggaggt gggccgccac
     1921 cgtgagggtc cagcgcgagt cgcactcgtc gtcgacgagc aggaccgggc cgtcgagcag
     1981 ggcgccttcg ggcagctggt cgggatcgac ggcgaagcgg ccccacacgt tggcgacctg
     2041 gtgggcggag ttggcctgat cggcctggaa gccgccgtcc tcgatgcgga ccagcgccgg
     2101 gtgcaccggc agcttgccga gctggccgag gcccgagcag agccggtcga tgaggccctg
     2161 gcgccggcgg gacggcaccg ggcagatcca accgggacgc tgctcccact cccagcgctt
     2221 cagcacgccg gcgaccgccc ggatcatgtc ctcgtgcagc agccgatcgc cggtgaggac
     2281 ctcgtcgatc atcggacccc agcccccgtc gcccacccgg cacagggcgc gaccccaccg
     2341 ggcctgcgcc tcgggcttga tgcggccctt gggctcgtcc agccccgacg gccactggcg
     2401 gcggggctcg accccgacgt cgccggcccg caggtgccgt cccgcttcgg ccaccagctc
     2461 ggggtcgaga tcggtgctgc ggaccgtgcc gaggcaacgg tcgcaccggc cgcagtcctc
     2521 ggcctcggcg tcgtcgaggg cctcgcgcag gaaccggagc cggcacccgg gacggtcggc
     2581 gaaggccagc atctgctcgg actcgtcccg gcggagccgg cggaggcgct cggctttctc
     2641 gcggtcgtag gtccagccct cgtcggcgag caggtagccg cccttcaccg cccggatggc
     2701 cccgtcgacc tcgaggatct tgagcagggt ctgcaggcgg ctgcgcccga ggttcacgac
     2761 cgactcgagg gtgggcaccg acgtgggccg tgacgacgag agctggagca gcacctcgtc
     2821 gcacgtggac tcgggcggca ggctgacctg ctcgaaccag cgccagatgg cggcgtcctc
     2881 ggtggggcgg gggagggcga cgacctcggc ccgctcgagg gcgcggccgg cgcggccgat
     2941 ctgctggtag tacgccaccg gcgtgggcgg caggccgagg tggacgcaga acgccaggtc
     3001 gcccttgtcg aagcccatgc cgagggcgga cgtggcgacc agcgccttga gctcgttggc
     3061 ccggagctgc tcctcgagca cgaggcgctg ctcgggctcg gtgcccccgg tgtaggcctc
     3121 gcacgcgtgg ccgcggaggc ggagccactc ggcagtctgc gcggcctgct cctgggtgag
     3181 gcagtagacg atgccggacc cgggcagctc gtcgaggtgc tcggacaccc aggcgaggcg
     3241 gtgggcatcg tcgggcaggt cgaccacgga gaggtgcagc gcctcgcggt cgagcgtggt
     3301 gcgcagcacg agggcgtcgg agccgagctg ggaggcgacg tcgtcggtga cgcgctggtt
     3361 ggccgtggcg gtggtggcca ggaccggcgt ccacgacggg agctcgtcga gcaggtggcg
     3421 caggcgcagg tagtcggggc ggaagtcgtg gccccagtcc gagatgcagt gggcctcgtc
     3481 gcacacgagc gccatcggct gctcgagcag caggtggaac acccgttcgc ggaagccctg
     3541 gttggccagc cgctcggggg agatgaggag caggtcgacc gagtcggccg tgatggccgc
     3601 ctcgatgcga tcccactcgt cggtgttgga ggagttgatg gtctcggcga cgaggccgag
     3661 ccgctgggcg gccgccacct ggtcgcgcat cagggcgagc agcggcgaca cgacgacggt
     3721 ggggccccag ccccggtcgc gcagcatgcg ggtggcggag aagtacacgg ccgacttgcc
     3781 gaagccggtg cgggccacga gcagggtgcg ccggccgtcg gccaccaccg cctgcaccgc
     3841 ggcgacctgc tccgggcggg gcacggcgtc gggcccggcc agggcacgga cgtgctgggc
     3901 gagctcggcc tcgacctcgg cggtcgtggg acgtggggga gcggtgaggg ccacggaggc
     3961 gaacctaacg agaccccctg acagtcaccg gaccggtctc cgtcagctcg tcctccgcgc
     4021 tgtcgaagcg ggcacgtgca gccgtggaca tcgcctcggt gtggaggagc caacgccccc
     4081 gcccgctcgc cttcgtcgcg tacatggcct cgtccgcagc gcgcagcagc tcctcggcgt
     4141 cgtcggtccc gtcggagatc accacgccga tgctcgacga catccggacc cggcgcccgt
     4201 cgaggtccat gggttcggcc agcgcgtcga cgaggtcatc gcagatcgcg gagacggccg
     4261 cctccggctg ctcgccgagg ccctcgagga cgaccacgaa ctcatcgccg ccgagccgcc
     4321 cgagcgtgtc ctgggcgcgc agccgcgacc gcacccgctc ggcgacctcg atgagcaggc
     4381 ggtcgccggc ggcatggccg agggtgtcgt tgacctgctt gaagtggtcg acgtcgaaga
     4441 acaggacggc gatctcggtc cccgaccgcg ccgcccgtcg cgcggcgccg tcgacgcgat
     4501 cgaccagcag ggcgcggttc gggaggccgg tgaggtcgtc gtgcagcgcc tggtggcgca
     4561 gggcggcggc gtcggcctcc cgctgcgtga cgtcgcggct gcacacgagc aggccgccga
     4621 ggagggggtc gtccgttcgg tccgagacgg tggcctccaa ccagcgccac tcctccaggg
     4681 cctgcgacca cacccggaac gtgcgccgct cggggatcgg tgcggtaccg gccagcgccg
     4741 cggccatctc gctgacgatg gcgtcgcggt cgtcgggatg catgaaccgc ccgaccgcgg
     4801 ccccttcgaa gtcgtcgacg ccgaacagcg ccacggccga gggcgacgcc caggagtagc
     4861 gcccgtcggg gccgatcacg tagatctgat ccgacatctg ctcgacgacc gcctccagca
     4921 tccgctgcgc ggcgacggcg tcggtgcggt cctcgatctg ggcgatgaag tgccggggcc
     4981 gcccatcggc ccagcggacg agcgacaccg actggcgggt gtgcacgatg gacccgtcgc
     5041 ggtgcacgta gcgcttgtcc accgagtacg tcggacggat cccggcgagg acctcgcggg
     5101 cgagggtgac gtcgccgtcc aggtcgtcgg ggtgggtcag ctcctggaac atccgggagg
     5161 cgagatcgga ggccgggtac ccgatcagct cgcacagcgt cgggttcacc cgcagcaggg
     5221 agccgtcgag ggcgacgacg gcgagcccga tcggcgacag gtcgaaggca tctccgaagg
     5281 cgtcgtgctc ggcgtcaccc gatggggagg aggcggcgtg tccgtccgcc gacgtgtgat
     5341 ccacctccac cacatcggca gggtgcgccc ggaggtgaag gattggcagc cacgcgggcg
     5401 gcgggtcctc ctcgttcagg cggggaccca gatcagcgcc agctggcgga cgtcgatgtc
     5461 gttcttctcc agcccgacct cgacctcctc gacctggtcg gcgacgtcgg cccacccggc
     5521 ctcgatctcg gcgagctcgt ccaccagctc ggcctcgagg tcggcgagct cgagcgtctt
     5581 ggcctcgacg ccggccaggg cggtctcgag gcgctgccca gccgccgacg tctgcttgcg
     5641 ccgtgaggac acacccttga ccttggacac gatcgatcgg gccgagcgac gcccgccgag
     5701 gaacgacccg agcaggtcgc cggcggcgga gacgagctcg tgctgcttgg cgtcgccggc
     5761 tcgggcctcc agctcggcga cccgggtctg ctcccggtcg atcgccttgc ggatggtgcc
     5821 catgcgccgc tcgagctggg tccggacctt ggcggcgtcg acctcggccg ctgcctcgcc
     5881 ggcggcccgg cagcgcacgg cgaagtcctc cgccgactcg ccgggacggg accacagctt
     5941 cagatcggcg ttgcgcagca cggttaccgt gcgctcggcg tggaggtggt cgacgaccgc
     6001 cgccttcgca tcgcggaacc aggtggcctt gccgatgggc gccgccggca gctcgtacag
     6061 cgcgggggtg ggggcagcct cgcgcaggtc gcggtcgtcg tagtcgaccg tgacgagctc
     6121 ggcggggtcc aggtgctcgc ccagccccac caggacgcac tcgaactcct ccgtgtggcg
     6181 caggtcggcc ttggtctcgt cgaacaggag cgagacccgc acggccaggc cggcgcggtg
     6241 gcgggtggac gacgggtcgc cgccgacctc ggggagccac ggggcggcgg ggtcgaccca
     6301 gcggacgggc acgccgtcgg cgaccgccgg cggcacgggg gtctcgtcgt cgccgacctc
     6361 cagcggccgc tcctcgaccg gcgtggtgct gctggtcgac gtggtcgacg tggccgccgg
     6421 cggcgcggcg gtcgcctggc ccggcagggc gcccacctgg tccttgctga gcggtccggc
     6481 caggtagtcg agcgcccagc gggtggtgaa caccgagggg gccgacgcct tggtggagtg
     6541 cagcacgaac tcgcgcttgc cgaggccggc gatggtggtc cgcacctgct ccgcgtccac
     6601 cgatccggac gccgcctgca gcccgtcgcg caggcggtcg acgtcgcgct cggtctggag
     6661 ccggccgatc atccaggtgc cggcgttcga gagcgccttg tagtccaggt ccaccgggtt
     6721 ctgggtcgac agcaccatgc cgacgccgaa ggcccgggcc tgcttgagga tggtgaggat
     6781 cggcttcttg gccggcggcg cagccgacgg gggcacgtag ccgaagacct cgtccatgta
     6841 gacgagggcc cggaggtccg gggtgcccga ctggccccgc atccaggtga ccagcttgga
     6901 cagcacgagc gtgacgacga actggcgctc ctcctccgag aggtgggcca ggtagatcac
     6961 cgagcacgcg gcccgaccgt cgtcggtcga cagcatcgag ccgatgtcga tggggacgcc
     7021 ctgggaccag gcggcgaagg acggggaggc gagcaacccg ttgagctgca tggcgagcgc
     7081 cgtccggtcc gccgccgggt agaacgactc gagctccagc acgccgagct tgcggatggg
     7141 cgggtcctgg acctggtgga ccagctggcc gaggtcgagg tcctcgccgg cggtccacgc
     7201 ccgctgcacg aggttggaga gcaagatgtg ctcccgcgac gacagcgggt ccgccgtgat
     7261 gccgaccatc gaaagcaggc cgctgaccag gccgtcgatc tcgtcggcct gggcctcctc
     7321 gtcggcgtcg ggcggtgcgg acagggagcc gaccaggtcc atggcgaccc cggccgtcga
     7381 tcccggtgtg tagacggtga cgtcgctgcg ctcgtcgagc tcggcgatgt gggtgccgtc
     7441 gaggccccag ccggccaggc cctcgcgcca ggtcgtggcg gtttcggcag cgtcggcgcc
     7501 gggcgcgacc cagggggcga actcctcgcc ggagaggccg gggaagcgca gcgccaggtt
     7561 ggtcatgtcg cccttggggt ccaggacgag ggtggggatg ccggcccgga gcgcctcctc
     7621 gatgagcacc acgccgagcc cggtcttgcc cgacccggtc atgccgacga tgaccccgtg
     7681 ggtggtcagg tcggaggcgt cgaccaccag gggcgtcccg gtccgctcgc gctcggcggg
     7741 gtcgatcgtc tcgcccaggg tcagctcggg gtccatgggc ggaacctacc ggcggtcgtg
     7801 gaggggtgtg tcgctgtcgg agcccgtggc tacggtgcgc cgacgtgccc ctcttccagg
     7861 tgacccccga agagatccgc atcgcgtgcg aggtcgagac ggccgaagag gccggtctcg
     7921 tgtgccgcaa cgtctacacg tggacggaga acgacctcct cgcccgggtg gccgactggc
     7981 tcgccaccgt gccgctccgg atcctgatca tcttcgtgat cgcgtgggtc gtgaaccgac
     8041 tggtgcgcaa gtccatcggc tccttcgtgg cgaacatggc cgccgacgac caccaagagg
     8101 cccgcagccg gctgcgcaag tacacgccga ggccgctgca gtccacgacc gcggtgcagc
     8161 cgctgcgggc ggcgtctcgc gcccagacca tcggcctggt gctgaagagc acggccagca
     8221 tcgtcgtcta ctcgatcgcc gtcatcaccg cgctggccga ggtcggcatc aacctggcgc
     8281 cgctcatcgc cggcgccggc atcgtgggcg tcgccctcgg cttcggcgcc cagtcgctgg
     8341 tgaaggactt cctgtccggt ctgttcatgc tggccgagga ccagtacggc gtgggcgacg
     8401 tcatcgacgt gggcgaggcc gtcggcaccg tcgaggtcgt gaacctgcgc accaccaagc
     8461 tgcgaggggt cgacggcacg gtctggcacg tgccgaacgg cgagatccgt cgggtgggca
     8521 acatgtccca gcagtgggcc cgcgccctga tcgacgtgga ggtcgcctac gacaccgaca
     8581 tcggcaaggc cgaagcggtg gtcaaagagg tcgccgacca gctgtggcgg gacgacatct
     8641 ggcgcaccga cgtgctcgag gaacccgaga tctggggcgt cgagaccctc ggtgccagcg
     8701 gcgtcatcat ccgcctggtc gtcaagaccc agcccggacg gcagttcgcc gtgagccgcg
     8761 agctgaaccg gcggatcaag gaccgcttcg acaccgaggg catcgagatc ccgttcccgc
     8821 agcaggtcat gtgggttcgg cgcgaagcgg gctcctccac cgacagcgcc ggtggtcccc
     8881 tcgaggatgg ttcggagggc gccggcgacg aggccggcgc cccgagcgct tgaccagcag
     8941 gcccgtccgg gcccgcgcgc tggaggccac cgccagcctg aaccccgggg cgttctcggt
     9001 gggcgactgg ggcctgttcg tcagcatcgc cttgatctgg ggctcgtcgt tcctgttcat
     9061 cgccatcgcg ctggactcga tcgagcccgg gctcatcacc tggggtcgcg tggcgttggg
     9121 tgccgccgtg ctcaacgtgg tgcccaaggc ccgcacccgc ctcgaccccg aggaccgctt
     9181 ccgcatggtg ctgctctcgc tgctgtgggt cggcatcccc ttcacgctgt tcccgctggc
     9241 cgagcagcac atctcctcgg cggtgaccgg catgctgaac gggtcgatgc cgatcttcgc
     9301 ggcgctggtc gccggcgtgc tcctgcgcac gccgcccggt ccgacgcagg ccgcgggcct
     9361 cgtcgtcggc ctcgccggaa cgctggccgt gtccctggac cagctgggcg agggggacaa
     9421 cgcctggatc ggcgtgctca tggccctcgg cgccaccgtc tgctacggct tcgccatcaa
     9481 catcgccgcc ccgctgcagc gccgctacgg atcgctggcg gtgatggccc ggatgctcgc
     9541 tctggccacg gtgtggaccg ccccgttcgg cctctggtcg atcgctggct ccacgttcga
     9601 gctcggcccg gcgctgtccg tggccttcat cggtgccgtc ggcaccggca tcgccttcgt
     9661 gatcatggcc accctcgtcg ggtcggtggg ctccacccgg gcgtcgatgg ccacgtacct
     9721 gatcccggtc gtcgccatgg tcctcggcgt cctggtgcgc gacgagcgcg tcggcgtcat
     9781 cgccgtggtg ggcgtggtgc tcgtcgttgt cggcgcctac ttcgcgtccc gtccggaacg
     9841 ggcggtgccg ccggccccga tcgccatccg cgagcccacc tgacggacgg ttgcagaaaa
     9901 ctggcagtga tggtgtagcg gcggctcagg cccggagcga caccaggtac tggccgccgc
     9961 cctcgtcgac ctcggcggtg gcgcccaggt tcggggccag gcgcccgacc cggtcggtca
    10021 gccacgcggc ggcggcgtcg gcgtcgtcgg cggtggggca gcgggccatg acctcccggc
    10081 cgcccttgcg ctcgaggcgg tcgacggcgg tgaggatgac ggcggggtcc acgacgaaga
    10141 ggtgctcggg
//
LOCUS       DATISU010000421         2273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_317005, whole genome shotgun sequence.
ACCESSION   DATISU010000421 DATISU010000000
VERSION     DATISU010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317005"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1634
                     /locus_tag="VLR27_16680"
     CDS             <1..1634
                     /locus_tag="VLR27_16680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HSP05145.1"
                     /translation="RLADTTFDVLVVGGGITGAGVALDAASRGLRTALVERDDFASGT
                     SSKSSKLVHGGLRYLQQGDVRLVYQALAERQRLRHNAPHLVRILPFLLPIFSRDGLFN
                     RKVAWALGKAMWMYDLTGGARIGKLHQRISKEETLAHMPTLPADRVGASYLYYDAQAD
                     DARLTLTVARTAAELHGAVCVNHAPVVELTHQAGRSTGAIVEDAESGDRIEVRARAVV
                     NAAGVWADDIRALDEGMHPHAIRPAKGIHLTVPWSLVRNDIAVIAPVPKDKRSIFVVP
                     WPAPDGEDPAFTYIGTTDTDYDGPTEDPQCTREDVAYLLDAINHSTTTEITERHVVGT
                     WAGLRPLVKASTTGRTADLSRNHAVVTSASGVVSVTGGKLTTYREMAADTMGAVAEVL
                     DLPRSQRRSRTARLRLLGADGYDPDATGPDQHLHQRYGGEARVLQAMAERDPSLAEPL
                     VAGLPHLRAEAVYAVRYEMATTLADVLERRVRGLMLSRDATADAAAAVAELIGPELGW
                     SDRRRADEVAELRTRATQQRDAPGLPDSVLPGIGS"
     gene            1636..>2273
                     /locus_tag="VLR27_16685"
     CDS             1636..>2273
                     /locus_tag="VLR27_16685"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HSP05146.1"
                     /translation="MVWGRRRRARRWLDGWEQVAETALPQWSLLDDSERARLGARVEH
                     LVRRKHWEAARGFELTQEVVITVAASAGLLALGLDRDCYRHVKAIVVHAKTFTSRGVR
                     ATHVRGVVTRGPQRLLGHTRDGRGPVMLSWRAIRTDIRRPERRQNVIVHEFAHKLDSA
                     GGLFDGTPEITDRADRADWVHVCNREYRRLRRRDEPDPVLRRYATKSPAEFFA"
BASE COUNT          317 a          891 c          783 g          282 t
ORIGIN      
        1 accggctcgc cgacaccaca ttcgacgtgt tggtcgtcgg cggagggatc accggcgccg
       61 gggtcgccct cgacgccgcc tcacgcggcc tgcggaccgc actcgtcgag cgcgacgact
      121 tcgcgtcggg cacgtcgtcg aaatcgtcca agctcgtgca cggcgggctc cgctacctcc
      181 agcagggcga cgtccgcctg gtgtaccagg ccctcgccga gcgccagcgg cttcgccaca
      241 acgcgccgca cctcgtgcgc atcctgccct tcctgctgcc gatcttcagc cgcgacggcc
      301 tgttcaaccg caaggtggcc tgggccctcg gcaaggccat gtggatgtac gacctcaccg
      361 gcggcgcccg catcggcaag ctccaccagc gcatctccaa ggaggagacg ctggcccaca
      421 tgccgacgct gccggccgat cgcgtcggcg ccagctacct ctactacgac gcccaggccg
      481 acgacgcccg cctcaccctc accgtggccc gcaccgcggc cgagctgcac ggggcggtgt
      541 gcgtgaacca cgcgcccgtc gtcgagctga cccaccaggc cggacggtcg accggggcga
      601 tcgtcgagga cgccgagtcc ggcgaccgca tcgaggtgcg ggcccgggcg gtggtcaacg
      661 cggccggggt ctgggccgac gacatccggg ccctcgacga gggcatgcac ccccacgcca
      721 tccgaccggc caagggcatc cacctcaccg tgccgtggtc gctcgtgcgc aacgacatcg
      781 ccgtgatcgc cccggtgccc aaggacaagc ggtcgatctt cgtcgtgccg tggccggcgc
      841 cggacggcga ggaccccgcc ttcacctaca tcggcacgac cgacaccgac tacgacggcc
      901 cgaccgagga cccccagtgc acccgtgagg acgtcgccta cctgctcgac gccatcaacc
      961 actcgaccac caccgagatc accgagcgcc acgtggtcgg cacgtgggcc gggctgcgtc
     1021 cgctggtgaa ggcgtcgacc acgggccgca ccgccgacct gtcccgcaac cacgccgtgg
     1081 tcaccagcgc gtcgggcgtc gtgagcgtca ccggcggcaa gctgaccacc taccgggaga
     1141 tggcggccga cacgatgggg gcggtggcgg aggtgctcga cctgccccgg tcccagcggc
     1201 gatcccgcac cgcacgtctg cgcctgctgg gcgccgacgg ctacgacccc gacgccaccg
     1261 gacccgacca gcacctccac cagcgctacg gcggcgaggc gcgggtcctg caggccatgg
     1321 ccgagcggga cccgtcgctg gccgagccgc tggtcgccgg gctgccgcac ctgcgggccg
     1381 aggccgtgta cgccgtccgc tacgagatgg ccaccaccct cgccgacgtg ctggagcgcc
     1441 gggtgcgtgg cctcatgctc tcgcgggacg ccaccgccga cgccgcggcc gcggtcgccg
     1501 agctgatcgg gcccgagctg ggttggtccg accggcgccg ggccgacgag gtggctgagc
     1561 tgcggacccg cgccacgcag cagcgcgacg cgcccgggtt gcccgactcg gtgctccccg
     1621 gcatcggcag ctgaggtggt gtggggccgg cgtcgccgcg ctcggcggtg gctggacggc
     1681 tgggagcagg tcgcggagac ggcgctcccc cagtggtcgc tgctggatga cagtgagcgg
     1741 gcccggctgg gcgcccgggt cgagcacctc gtgcgccgca agcactggga ggcggccagg
     1801 ggcttcgagc tcacccagga ggtcgtgatc acggtcgcag ccagcgccgg cttgctggcg
     1861 ctcggcctgg accgcgactg ctaccgccac gtgaaggcca tcgtcgtcca cgccaagacc
     1921 ttcaccagcc ggggcgtgcg ggcgacccac gtccgtggcg tggtgacccg cggtccccag
     1981 cgcctgctcg gccacacccg cgacggtcgc ggcccggtca tgctctcgtg gcgtgccatc
     2041 cgcacggaca tccgtcgccc cgagcgccgc cagaacgtga tcgtgcacga gttcgcccac
     2101 aagctggact ctgccggcgg cctcttcgac ggcaccccgg agatcaccga ccgcgccgac
     2161 cgggccgact gggtccacgt ctgcaaccgc gagtaccgcc ggctccgccg ccgggacgag
     2221 cccgacccgg tcctgcgccg ctacgccacc aagagcccgg ccgagttctt cgc
//
LOCUS       DATISU010000422        25340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_269590, whole genome shotgun sequence.
ACCESSION   DATISU010000422 DATISU010000000
VERSION     DATISU010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 25340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25340
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269590"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..80)
                     /locus_tag="VLR27_16690"
     tRNA            complement(7..80)
                     /locus_tag="VLR27_16690"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44..46),aa:Val,seq:gac)
     gene            168..647
                     /locus_tag="VLR27_16695"
     CDS             168..647
                     /locus_tag="VLR27_16695"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HSP05147.1"
                     /translation="MPAAIDDLDRRLLQCLAEHPRAGVMELARQLEVARGTVQARMEK
                     LQQRGVVTGYGPDIDLRSIGYDVIAFVTLEIAQGALETVVAHLSTLPEVLEVHTTTGP
                     GDLLCRVVAPTNADLQPLIGRMLATGGIERTTTQIALTEQIPYRVLPLVAEPGDGSA"
     gene            693..890
                     /locus_tag="VLR27_16700"
     CDS             693..890
                     /locus_tag="VLR27_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05148.1"
                     /translation="MATVAAMRILAHQGGWDEILFVLAPLLIFGGLLALARKRVGEIE
                     QDGATPGGDEPREASKGSDST"
     gene            complement(918..2096)
                     /locus_tag="VLR27_16705"
     CDS             complement(918..2096)
                     /locus_tag="VLR27_16705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP05149.1"
                     /translation="MNRPPRSGPVPSHRRGQRWAVTAVFFVNGVTFASWLPRIPEVRD
                     RLDIGLGELGVVLLGTGLGGLLASAAGGALVDRLGSRRSCVTAGVLLSLGLPLIALAS
                     TPVALGAVLVALGSVDVLTDIGMNVQAAQVQRESSASVMQRFHGAWSVGALTGAAVAS
                     GAAAVEVSLGRQLVATSVVLISTITYASRHLSAHDDAPAPQPDGTRRAPVLALLAAVA
                     LAMAIVEGAPGEWAAVFGADVHDAGPGAAGLGFLAMSAGMVTGRFTGDLATDRLGPTR
                     VLRVALSLVGVGLAGVVLSPGLAVAVAGFAVIGLGVSVLFPALYLSAADRDGIPAGLG
                     LGVMSTGARFGFLVSPPLVGALAGATSLRIALGAVVGTAAVAALGLEAALSRASRTAA
                     "
     gene            complement(2093..3019)
                     /locus_tag="VLR27_16710"
     CDS             complement(2093..3019)
                     /locus_tag="VLR27_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSP05150.1"
                     /translation="MGLCDGRVAIVTGAGRGIGREHALMLAEHGAAVVVNDVGGAPDG
                     SGTDATPAEQVVAEIEGMGGRAVANADDVADWDGAKRMVDQAIDSFGRLDVLVNNAGI
                     LRDRMLVNMSETEWDAVVRVHLKGTFAPARHAAEHWRNQAKAGEEVDARIINTTSVSG
                     IYGNIGQTNYGAAKMGIAGFTIIAALELARYGITVNAISPGALTRLTEGLRAEPPSEA
                     DREAQSPRWIAPICTWLASPLSKNVTGRVFQVHGQEIAIAESWHRGPTTDNVDDPEAM
                     TDVVARLMADARPNADMHGDDAEGPGRPGRTI"
     gene            3165..4985
                     /locus_tag="VLR27_16715"
     CDS             3165..4985
                     /locus_tag="VLR27_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05151.1"
                     /translation="MPENRTNRSRARIAAALVAAAVGAATFTTGPGSAGAAAPPSSGA
                     PPQVLEERMNGEAAIRALGERLPEVARANGISADELRRDLRADDELWLDETGALLFID
                     EGIAAHAELATSNVTDPAWLDHATTDALTLHSRPGATRVIHLDFDGHDPSGSIWKDKT
                     PAQPYDTDGDPATFSDQERRVIIEVWQRVAEDFAPFAIDVTTEDPGIDAIRRTDSADQ
                     LYGTRVVVTPTATNCESCGGVAYLDVFDAFGGSASTGWTHDLYQPAWVYLNDFSAKSI
                     AEAASHEAGHNLDLMHDGTSTIGYYEGHGDWAPIMGVGYYREVSQWSRGEYAGANNSE
                     DDLAVIAANGGVPAADDHGDTLATATPLDSVIDVIGHIGTRADVDAFSFTTAGGALSL
                     TASPVAVGANLDIGLRLVDDSGAVVASVDPAGLGASLDATVPGGTYAVVVDGVGVGDP
                     RTTGYSDYASVGRYRLTGALPDIAGDGSDPDPDEPDPEPELAAPSTPSLTATEADGSV
                     TLDWGDDPTAAPTFAVYRESRHKNGSYRGRTLLGEGTESTWVDTPGSGTFRYQVVATN
                     SSGSAESDLIEVTVTSTTSDGPTKGGKGGGGGKSGAMKTR"
     gene            complement(5118..5495)
                     /locus_tag="VLR27_16720"
     CDS             complement(5118..5495)
                     /locus_tag="VLR27_16720"
                     /inference="COORDINATES: protein motif:HMM:NF039494.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HSP05152.1"
                     /translation="MTTSPTLRRRARRLPLLAVFGIALLFGAACTPQEAVWMHFADNG
                     GSGELHAQAQRVVSCESGWNPTAVSPTNDHGLFQINRRWHEASFTRVTGQSWDKVYDP
                     YWNALYARHIVNTQGWSPWTCRP"
     gene            5765..5938
                     /locus_tag="VLR27_16725"
     CDS             5765..5938
                     /locus_tag="VLR27_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05153.1"
                     /translation="MEIMGRFRNRTETVERTTSDDAHFVLIETDDHEQIVLAGSSGDE
                     MQAKLDDFLTWAS"
     gene            5955..6458
                     /locus_tag="VLR27_16730"
     CDS             5955..6458
                     /locus_tag="VLR27_16730"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HSP05154.1"
                     /translation="MSIEVRQADITTLAVDAIVNAANESLLGGGGVDGAIHRAAGPGL
                     LAECRTLGGCPTGDAKVTSGHDLPARWVIHTVGPVWRGGDHDEAELLASCYHRSMAEA
                     ARLGPASIAFPAISTGVYGFPPDRAARIAVDAIRAELDLDGRPTEVVLCAFSADAADL
                     LEDALRL"
     gene            complement(6560..6634)
                     /locus_tag="VLR27_16735"
     tRNA            complement(6560..6634)
                     /locus_tag="VLR27_16735"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6599..6601),aa:Cys,seq:gca)
     gene            6729..7505
                     /locus_tag="VLR27_16740"
     CDS             6729..7505
                     /locus_tag="VLR27_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05155.1"
                     /translation="MARQPAPPGPPPPHYRPPGSPRPLPGALTPAPAPRPAPSGVSPL
                     AATAVAVLTLLVGLVAGFFLGRAYDDGASVSATSTGTIAPSPTTPTSPPATPPGDTIP
                     QQPPGAPPATDLAPGNLGEFDEPIPAGQAYVLGLYEIEVLGVTRDAGDALAEFAPANP
                     PPPSGQQHVLVELAVRFTDPTGLGSPGSIPFFVSDGDGEWASFEAGCGLVPGDLRDTG
                     FIEVGDEAVGQVCFTVPSDAVDELALGTESFAGPLWFALP"
     gene            complement(7511..8332)
                     /locus_tag="VLR27_16745"
     CDS             complement(7511..8332)
                     /locus_tag="VLR27_16745"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSP05156.1"
                     /translation="MQTAPGTTAVITGAASGIGLALAQRAAADGMNVVLADVDEAGLE
                     SAAGSLDGAPDGGVLTACCDVSDPDAVEDLAARAYDRFGSVELLCNNAGVGGGGTVAE
                     LSRKDWEWVLGVNLWGVIHGVTSFLPRMLESGGPAHVVNTASMAGFFATAGMAPYAVS
                     KFGVVALSESMRAELAGTPVGISVLCPGWVSTRIHESERNRPAELTVERPPRAEELRD
                     MIRQVIESGLPASSVADAVFAAIQADRFWIFTHPEMVRATAARQEEVLAALDDLH"
     gene            8370..8801
                     /locus_tag="VLR27_16750"
     CDS             8370..8801
                     /locus_tag="VLR27_16750"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HSP05157.1"
                     /translation="MQIAVVYASRTGNTRRAAELVGTAIAERGHTVSVASVDDLDYKV
                     LADADLVVLGTWVAGHFVIGQKPGDARRLAKVPKLTGKPVAIFLTYAINPGSALGKLE
                     KALTREGADVIGGYAWKRSNLPAGVEDFVDGVLGAVKPASA"
     gene            8798..9673
                     /locus_tag="VLR27_16755"
     CDS             8798..9673
                     /locus_tag="VLR27_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP05158.1"
                     /translation="MSAPGWFDRALAHTPETRQVQVDGVGISYLAWGRVAAPGLVFIH
                     GGAAHAHWWSFLVPLLADSYRIAAMDLSGHGDSDRRTEYPMSGWAAEVMAVADDLGAA
                     GAPVVVGHSMGGFVAIATGATYGEDLAGIVILDSPVSAPDPETDAARGGKVFGPLRTY
                     PDLDTALGRFRTVPEQQHYLPFVMDHVARHSLREVDGGWSWKYDPEIFVPTRAESREL
                     LARVSCRVALFRAEHGLVTPEIGQYMYDQLGRVAPVVEIPEAGHHLMLDQPLLLLTAL
                     RTLLADWEHSSPRTR"
     gene            complement(9680..10105)
                     /locus_tag="VLR27_16760"
     CDS             complement(9680..10105)
                     /locus_tag="VLR27_16760"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP05159.1"
                     /translation="MTASQPSSPTEDAFQTSFPPRTDALARARTAFSAWLRRRTQGET
                     VDELEIVFSELAANAVDASPDGAEEVTGRAWCEGSELVLDLVNRTDATEEPVARWDLD
                     DPLRAGGRGLVITEALVDSMEVRLAPGQRLVVRCRRRLE"
     gene            complement(10262..10335)
                     /locus_tag="VLR27_16765"
     tRNA            complement(10262..10335)
                     /locus_tag="VLR27_16765"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10299..10301),aa:Gly,seq:gcc)
     gene            complement(10383..10616)
                     /locus_tag="VLR27_16770"
     CDS             complement(10383..10616)
                     /locus_tag="VLR27_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05160.1"
                     /translation="MADNLDVDAMLARFKERAAAVKKRNLPPVAGEERTRFIEQAKTD
                     FMDYAIIADAEGHLEDGILTLTVDLRPEDERGT"
     gene            complement(10638..11276)
                     /locus_tag="VLR27_16775"
     CDS             complement(10638..11276)
                     /locus_tag="VLR27_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05161.1"
                     /translation="MPAAEPHPRRRVALRRALSLLLAASAAILGPGASGAAGQILPTS
                     TTAPATTTTEAPVETTTTEAEAEAEPEPEETTTTTRVTGTTARPTTTAGSDDEDDGDD
                     GVVTEEDVAEDAGTVADDEPAETTSTARDLLVSGDGSDGAQATTTSSTTVVEVAADED
                     GALDEQAQIWLIVAGLVLVALLIGVWTWRYWVRTRPATPDEGPDDTTVFHRG"
     gene            complement(11324..12100)
                     /locus_tag="VLR27_16780"
     CDS             complement(11324..12100)
                     /locus_tag="VLR27_16780"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HSP05162.1"
                     /translation="MPELIEVETYRRQAEAVVGRTITDAPVLDPLGLRGADPDVVRAE
                     LVGHRIEGAERIGKLLLLPTDGPVLGLRFGMTGRLLVDGGATIDGLLYGGQGDDPAWD
                     RYVLTFSDGTDLRIRDPRRLGGLQLDPDTSRLGPEASTVTAAQLRTALAGSRTAIKAR
                     LLDQARLAGLGNLLVDETLWRAGIDPARPCDEIADDEVPLLAEAIRTTVAELMQRGGS
                     HTGDLQPARVPGATCPRDGAVLDRATVGGRTTYRCPRHQR"
     gene            complement(12114..13271)
                     /locus_tag="VLR27_16785"
     CDS             complement(12114..13271)
                     /locus_tag="VLR27_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HSP05163.1"
                     /translation="MSDDAHRETSAIRAGRGPNQGSVASPIWPSTAYELRDLDVSARM
                     AVTPRATEFYARNGTPTVQAFADAVAEVEGAEAGIAFGSGMGAASSVVFALCSPGDRI
                     VAQASMFSVTNQLLTRTCERFGIEVALVDAFDADALRAAVAEKPTQLVWCETPANPAM
                     GIVDLEAVGGLMGPFTVVDSTMATPAVQNPHDHGVHLVFHSATKAIAGHNDALLGVVT
                     GERELIDVLWAHHVVHGAVASPFDAFLGLRGLRTLHVRQRQACETAHVLAERLDAHPA
                     VERVRYPGLASHPQHELAMRQMRSGGSIVVVDLAGGYEAGRAFVERIELAIPAVSLGG
                     PETLVTHPASMSAASLSPDERVAMGIPEGMLRISVGLEHVDDVLADVLAAL"
     gene            complement(13268..14575)
                     /locus_tag="VLR27_16790"
     CDS             complement(13268..14575)
                     /locus_tag="VLR27_16790"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HSP05164.1"
                     /translation="MTTDGEVRDERTTTSSANGARGGPLSGVVDPDRPLADLGRRAHP
                     STLPASGAWQDGDEIGNRRFAPITPGRPFALEGGGQLEGAVVAYETFGTLDADASNAV
                     LVCHALTGDSHVAGAAGPGHLSPGWWDGMVRHGSGIDLDRFFVVCVNVLGGCQGSTGP
                     ASPHPDDGRPYGARFPVVSVRDMVRTQRVVADELGIRRWHAVIGGSMGGMQALEWAIM
                     HPERVGSLIVVASTAAASAQQIGWWSTGRRIIRMDPAWRNGDYYDAAPGDGPHEGLAL
                     ARMVSQITFRSDDVFTDRFGRELVEPLDGFHLWQRFQVERYLEHHGDKLARRFDANSY
                     LVLTKAMDLHDIGRGRGGIDAAMARVRCPVVTVGISSDILYPAHQAAEIAALARAAGL
                     PAHYHRLESPHGHDAFLIEHEEMRDVLAPHLDDPTATSHRSTT"
     regulatory      complement(14626..14743)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            complement(14799..16379)
                     /locus_tag="VLR27_16795"
     CDS             complement(14799..16379)
                     /locus_tag="VLR27_16795"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HSP05165.1"
                     /translation="MPSVAATLAGAFATATRREGFVPDPALRLLHGPVPLDGDLAVDD
                     PHDLGPLHERLVGGTERTRRGAWYTPRWLAEDLVRRAVVADGTVVDPSCGGGVFLLAA
                     ADHLVAGGLDPTDAVGRLWGCDVDPLAVAVCEAALWWWSARAGCPIRMGESLVVGDAL
                     TEVALPPAGSVVGNPPFLGQLRSSTSADADRRAALRARFGDAVRAYTDPAWLFALTAV
                     EQVAPGGHVALVQPQSFLAARDAAAVRDRIDACADLVHTVVDDGGAFAANVQVCAPVF
                     RRRPEGSRVEPNDWVAPLADAAGIPSVALTSDRRLGSLATVHAGFRDEYYGLVDAVSE
                     SEADGGEARPRLVTAGGIDPCRLLARGQRFAGTRWTAPVVHTSSLQGRTARWAAVQSG
                     PKLLVATQTRVLEAVADPDGSLLGSVPVLCVRPHDADALWHLLAALHAPAATAWLFRR
                     SAGTALSADACRPTVALLSALPVPEDGPAWDLAADAAQAASVGDADVTEVARLADAAL
                     GVDDPDLLAWWDARRPRR"
     gene            complement(16393..17559)
                     /locus_tag="VLR27_16800"
     CDS             complement(16393..17559)
                     /locus_tag="VLR27_16800"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSP05166.1"
                     /translation="MTTFAELGIDDDLVECLASQGIESPFPIQELTIPDAVAGRDVCG
                     KAKTGSGKTLAFGVPMVMALPEPDDIHKGPAGLVLCPTRELALQVAEVLEPLAKVKGH
                     TITAVYGGEKIEKQIKSIDDGTGIIVATPGRLIDLLQRGDVKLDEVKMVAVDEADRMA
                     DMGFLPQVEWVLRHVPEGHQTLLFSATLDGSVGALIDRYQTDPVTKEVVSETVTVDQM
                     HHRFIKIHERDKAKVVAAIVENGNRTLIFSNTKRAADRLADDLDELGVKASPIHGDLP
                     QRAREKALQRFTDGKIQALVASDVAARGLDIDLIDTVIHWDPPPDHKTYLHRSGRTAR
                     AGTTGVAVTLIKWDEEMSVKLLQRRLGLDEHPMVEMFSNDERLKDLAGWDPFAG"
     gene            17620..17856
                     /locus_tag="VLR27_16805"
     CDS             17620..17856
                     /locus_tag="VLR27_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05167.1"
                     /translation="MREHLTKAQVEALVARLDGPPDALAEALADAVEALHGQRDVAGV
                     LDSLGRHHDATGVRTRDEATLWALATELNERRYL"
     gene            17903..18520
                     /gene="nthB"
                     /locus_tag="VLR27_16810"
     CDS             17903..18520
                     /gene="nthB"
                     /locus_tag="VLR27_16810"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR03888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HSP05168.1"
                     /translation="MELAHDLGGRDGFGAPIVEEDEPVFHAAWERTARALVFAAMGAH
                     PNPTTSAFRHAIERMDPEHYLASSYYEHWLTAAASIAVETGVVTREELEASAGGAFPL
                     ARPEVRPHVDGSGGQRFAVGDRVRVVAVEHGGHTRCPGYLRGRVGTVTRVDERFSLPD
                     VEAHSTRRVVEGTYGVRFSARDLWADADQHAAVHVDLWDSYLEAT"
     gene            18517..19110
                     /gene="nthA"
                     /locus_tag="VLR27_16815"
     CDS             18517..19110
                     /gene="nthA"
                     /locus_tag="VLR27_16815"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR01323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HSP05169.1"
                     /translation="MTADHHHPTEPAPIERRVAALEQLLTERGLVTPGLVDEVNRAYE
                     EDIGPRNGAQVVARAWVDPEYKRRLLLDGTAAIAELGFGGAEGDHMQVVENTDAVHNV
                     VVCTLCSCYPWPVLGLPPAWYKDPPYRARMVREPRVLLAEMGCQVDDGVEIAVWDSSA
                     EQRYLVLPQRPAGTDDLAEDDLAALVTRDAMIGVERL"
     gene            19107..19385
                     /locus_tag="VLR27_16820"
     CDS             19107..19385
                     /locus_tag="VLR27_16820"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05170.1"
                     /translation="MSGLDRIEVPRRNGELAFDAPWQARVFGLAAVLVETRFDGDWEP
                     FRSELITAIAADEARPYWESWTAALEGLLVAAHLLSVDDLDAATSSRP"
     gene            complement(19398..19679)
                     /locus_tag="VLR27_16825"
     CDS             complement(19398..19679)
                     /locus_tag="VLR27_16825"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HSP05171.1"
                     /translation="MATTFEDAVLAVLAALPAGEVITYGEVAAEAGRPGAARAVGRVM
                     ATSGADVAWWRVVAADGRLAPGKEAEQRRRLVAEGVDVGDRRVRGMRRR"
     gene            19704..20522
                     /locus_tag="VLR27_16830"
     CDS             19704..20522
                     /locus_tag="VLR27_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HSP05172.1"
                     /translation="MRTLTDADADRHAAEIEERGYTIVEDAFDATFATEVLDALDAVI
                     EQRQLTPADNGFEGRDTYRVYNLLAHDPLFQRIPTAPAVLPVVERVLDRECLLSTLSS
                     IDIRPGESAQPIHSDDQVIGLPRTGFPVVCNSMWAITDFTEANGATRLVPGSHRDPEY
                     PVYGETYDTIPAEMPAGSVLLWHGSLWHGGGANRSDERRVGIADNYCAGMIRQQENQQ
                     LGVPRHIAATFDKRLARLCGYGTYHGLIGHIDKTDPLRLLDRDDADDTMLWDAG"
     gene            20525..21349
                     /locus_tag="VLR27_16835"
     CDS             20525..21349
                     /locus_tag="VLR27_16835"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSP05173.1"
                     /translation="MAALDQIDRWDVPTVAAGVLGPDGLLAGRGETDQAFPLASVSKV
                     LAAIAVHVAVEEGTVGLDDDVTDAGGPEGATVRHLLAHASGLPPDGDRTPLGPPERKR
                     IYSNVGFEVLGDHVASRTGMAMTDYVRAALVDPLGLGATTIDGSPAHGYASSVDDLAS
                     ILVALHGGRLLAPSSIEAMTTPAFPDLAGVLPGYGAQDPNPWGLGVEVRGTKDPHWTA
                     PANSPETWGHFGRSGTFLWWDPTVGVGLVVLADREFGQWAIDAWPQLAADVVAEHG"
     gene            complement(21359..22453)
                     /locus_tag="VLR27_16840"
     CDS             complement(21359..22453)
                     /locus_tag="VLR27_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05174.1"
                     /translation="MERSPFPHSGPLEADEVTGRDDEAAELVRLLGGRVPIALVAPRR
                     YGKTSLLRRGAWLLDQVEPTTTVWLDLYGLSSVSDFAVRLDRALGACTGRFREALDRV
                     AGGLRISIGVVSAELRRSARSAPDATATVHALLESLVRVAEHHRTVLVIDEVADAIGV
                     GNVLELMRSHLQPVYRELGLAFAGSKPTMMARLFGDTDQPFYSQARRIELLPLPVEAV
                     VDIVHRGFRSTGRDAGPVASSIAAFGAGHPQRSMELADAAWWLTEPGGIAGPDTWDEA
                     LAGVRSRVAPGMRDFFGMLTSTQQAVLRAVVRTGAPFAASEGRFHDLSRSSITIARDS
                     LVDAGHLLFGLGGSSLTDPLLADWVARELP"
     gene            complement(22549..24345)
                     /locus_tag="VLR27_16845"
     CDS             complement(22549..24345)
                     /locus_tag="VLR27_16845"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HSP05175.1"
                     /translation="MADYTAPLDDIRFALDRIAKLGELSSLEGFGHAEPEMVHSILEE
                     CARFMEDKVAPLNRVGDIETSVWHAEDNSVTTPTGFIDAYKDYVKAGWGGVPFPEEFG
                     GGGFPWLVGIAMQEMMTAANEAFSMAPLLSQGAIDALLEHGDETIKETWLPKMVSGEW
                     TATMNLSEPQAGSDVGAVRTRAERQDDGTYRITGTKIWISWGEHDMADNIVHLVLART
                     PDAPPGTKGISMFIVPKVLIDDDGNLTDRNGVVCQSIEHKLGIKASPTCVLDYDGSVG
                     YIVGEENRGMAYMFTMMNNARLSVGLQGLAVGDRAYQDAVQYANERQQGRAIGAPAGE
                     QSLIIEHPDVRRMLLTMKAQLEAVRAVIYYTAAGIDRADRHEDEQTRTTAQERVDLLI
                     PVCKGWGTDLGVDITSTAIQVFGGMGYVEETGVAQHFRDARIAPIYEGTNGIQAMDLV
                     GRKLPMRAGGVVKDFLADVGATVEKGRAAGGDHAVIADALADALAALTESTDWLMANG
                     LEDVRHALAGATPYMRQFGIVAGGWLLAESALDAAELAAGDGGGFSSEFLEAKQTTVR
                     FYATQLLPQAGGLTGAVTAGFGDLMALTPQQF"
     gene            24402..24878
                     /locus_tag="VLR27_16850"
     CDS             24402..24878
                     /locus_tag="VLR27_16850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP05176.1"
                     /translation="MGPTDAMPPTVHRARFADLSPQDLYGILRLRSDVFVVEQDCVFL
                     DLDERDHEADAEHLWTTDGSGVVATLRLLHEGDGTWSIGRVVARADARSRGVAAHLLQ
                     AGLDRLRELGAETVRIGAQAQLRDWYARFGFAQSGPRYLEDGILHLPMSLSLGADR"
     gene            24875..>25340
                     /locus_tag="VLR27_16855"
     CDS             24875..>25340
                     /locus_tag="VLR27_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05177.1"
                     /translation="MSLVTLDSGILAWLAEDPGDGRPNAGLVVDADAATAIDTLCVPS
                     QTAAFVAAIDVLGLRVRRTVLTASTIEFVGGTAMFTMSGMYGRRQTSVHLDQPPTPDI
                     YRRLLPDVADEFDDELTTKPVSHIVADRVQLTPAISLHPCSGAQEENLVAVVD"
BASE COUNT         3530 a         9271 c         9185 g         3354 t
ORIGIN      
        1 tccgagtggg ccgtgaggga ttcgaacccc cgaccccctg cgcgtcatgc aggtgctcta
       61 gccaactgag ctaacggccc tcgggagcgg gcactgtagc agcgcgcccg ggcgccgcca
      121 catcgtcgat cggcccctcc gccacggctg tggtagaccg ccgagccgtg ccggcagcca
      181 tcgacgacct cgatcgacga ctcctgcagt gcctggcgga gcacccccgg gccggcgtca
      241 tggagctggc ccgccagctc gaggtcgccc gcggcaccgt gcaggcccgc atggagaagc
      301 tgcagcagcg cggcgtggtc accggctacg gacccgacat cgatctgcgc tcgatcggct
      361 acgacgtcat cgccttcgtc accctcgaga tcgcccaggg ggccctcgag acggtggtcg
      421 cccacctctc gacgctgccc gaggtgctcg aggtccacac caccaccggc cccggcgacc
      481 tgctctgccg ggtggtcgcc cccaccaacg ccgacctcca gccgctcatc gggcgcatgc
      541 tggccaccgg cggcatcgag cggaccacca cccagatcgc gctcaccgag cagatcccct
      601 accgggtgct gccgctcgtg gccgagcccg gcgacggcag cgcctgacgg cgcgagtgga
      661 ggacccggcg cagccacccc taccgtgccg ggatggccac cgtcgcggcg atgcggatcc
      721 tcgcccacca gggcggctgg gacgagatcc tgttcgtgct cgctccgctg ctgatcttcg
      781 gcgggctgct cgccctcgcc cgcaagcggg tcggtgagat cgaacaggac ggggcgaccc
      841 cgggcggaga cgagccccgc gaggcgagca agggctccga ctccacctga tcagcgcgcc
      901 cggatctgca gcgagcctca ggccgcggtg cggctcgctc ggctcagcgc cgcctccaga
      961 ccgagcgccg cgaccgcggc ggtgccgacc accgcaccca gcgcgatccg caacgaggtc
     1021 gcgccggcga gggcccccac gaggggcggc gacacgagga agccgaagcg agcgcccgtc
     1081 gacatgacgc ccagcccgag cccggccggg atgccgtcgc ggtcagcggc cgagaggtag
     1141 agcgccggga acaggaccga cacgccgagc ccgatgaccg cgaagccggc gacggcgacc
     1201 gcgagacccg gcgacaggac cacgccggcg agaccgacgc cgaccaggct gagcgcgacg
     1261 cgcaggaccc gggtgggccc gagccggtcg gtggcgaggt caccggtgaa ccggcccgtg
     1321 accatgccgg ccgacatggc caggaacccg agcccggcgg cacccggtcc agcgtcgtgg
     1381 acgtcggccc cgaagacggc agcccactcc cccggtgccc cctcgacgat cgccatggcc
     1441 agtgcgaccg cggccagcag ggccaggacc ggcgcccgcc gggtgccatc gggctggggc
     1501 gccggtgcat cgtcgtgggc cgagaggtgg cgggacgcgt acgtgatcgt cgagatcagc
     1561 accacggagg tggcgacgag ctgccggccg agggagacct ccaccgcggc cgccccggac
     1621 gccaccgccg ccccggtcag ggcaccgacg ctccacgcac cgtggaagcg ctgcatgacg
     1681 gatgcggacg actcgcgctg gacctgcgcg gcctggacgt tcatgccgat gtcggtcagc
     1741 acgtccacgg agccgagggc gacgaggacc gccccgagcg cgaccggcgt ggaggccagg
     1801 gcgatcaggg gcaggccgag ggagagcagg acgccggcgg tgacgcagct gcgccggctc
     1861 ccgaggcggt cgacgagggc accgccggcg gcgctggcca gcagcccgcc gagcccggtg
     1921 ccgagcagca cgaccccgag ctcaccgagg ccgatgtcga gccggtcccg cacctcgggg
     1981 atgcggggca gccaggacgc gaacgtcacg ccgttgacga agaagaccgc ggtgaccgcc
     2041 caccgctgcc cgcggcggtg ggacgggacc gggccggacc tgggcggtcg gttcagatcg
     2101 tgcgaccggg ccgccccggt ccctccgcgt cgtcgccgtg catgtcggcg ttggggcggg
     2161 cgtcggccat cagccgggcc acgacgtcgg tcatcgcctc cgggtcgtcg acgttgtcgg
     2221 tggtcgggcc ccggtgccac gactcggcga tggcgatctc ctggccgtgc acctggaaga
     2281 cccgaccggt gacgttcttc gacagcggcg acgccagcca ggtgcagatc ggcgcgatcc
     2341 agcgaggcga ctgcgcctcc cggtcggcct cggacggcgg ttcggcccgc aggccctcgg
     2401 tgagccgggt gagggcgccg ggcgagatgg cgttcacggt gatgccgtag cgggccagct
     2461 cgagggcagc gatgatcgtg aacccggcga tgcccatctt ggccgcgccg tagttcgtct
     2521 ggccgatgtt gccgtagatg ccagacaccg aggtcgtgtt gatgatgcgg gcgtccacct
     2581 cctcgccggc cttggcctgg ttgcgccagt gctcggcggc gtggcgcgcc ggagcgaagg
     2641 tgcccttcag gtggacgcgc accaccgcgt cccactcggt ctcgctcatg ttcacgagca
     2701 tccggtcccg gaggatcccg gcgttgttga ccagcacgtc gaggcgcccg aacgagtcga
     2761 tcgcctggtc gaccatccgc ttggccccgt cccagtcggc cacgtcgtcc gcgtttgcga
     2821 cggctcggcc gcccatgccc tcgatctcgg cgaccacctg ctcagcgggc gtggcgtctg
     2881 tgccgctgcc gtccggcgct ccgcccacgt cgttgaccac cacggccgca ccgtgctcgg
     2941 ccagcatcag cgcgtgctca cgcccgatgc cccggccggc accggtcacg atcgcaaccc
     3001 gtccgtcgca caaccccatg tcgcattccc ctcgtctcag cgtccagcaa tcgtggcggc
     3061 aaggtagcgg cagggccacc gcgccaaggg cgacggtacg aagtgctcaa cccggcaggg
     3121 gaaacagccg atggtcctgg ccagagggaa cccagggagg gaccgtgccc gagaaccgca
     3181 ccaaccgcag ccgcgcgcgc atcgcggcgg cactcgtcgc tgccgccgtc ggcgcagcca
     3241 cgttcacgac cggacccggc agcgccggag ccgccgcacc cccatcgtcc ggggcgccac
     3301 cgcaggtgct cgaggagcgc atgaacggcg aggccgcgat ccgtgcgctc ggcgagcgcc
     3361 tcccagaggt cgcccgcgcc aacgggatca gcgccgacga gctgcggcgc gacctccgcg
     3421 ccgacgacga gctgtggctc gacgagaccg gcgccctgct gttcatcgac gagggcatcg
     3481 ccgcccacgc cgagctggcg acgagcaacg tcaccgatcc ggcgtggctg gaccatgcga
     3541 ccaccgatgc gctcacgctc cactcccggc cgggcgccac ccgggtcatc cacctcgact
     3601 tcgacgggca cgacccgtcc gggtccatct ggaaggacaa gacgccggcc cagccctacg
     3661 acaccgacgg cgacccggcg accttctccg accaggagcg gcgggtgatc atcgaggtgt
     3721 ggcagcgggt agccgaggac ttcgcgccgt tcgccatcga cgtcacgacc gaggaccccg
     3781 gcatcgacgc gatccgacgc accgactccg ccgaccagct gtacggcacc cgcgtggtcg
     3841 tcacacccac ggccacgaac tgcgagagct gcggcggcgt cgcctacctg gacgtcttcg
     3901 atgccttcgg cgggtcggcc tcgaccggct ggacccacga cctctaccag ccggcgtggg
     3961 tgtacctgaa cgacttctcg gcgaagtcga tcgccgaggc cgccagccac gaggccggtc
     4021 acaacttgga cctcatgcac gacgggacct cgaccatcgg gtactacgag ggtcacggtg
     4081 actgggcccc gatcatgggg gtcgggtact accgggaggt cagccagtgg tcgcgtggtg
     4141 agtacgccgg tgcgaacaac tccgaggacg acctcgccgt catcgccgcc aacggtggcg
     4201 tgccggcggc ggacgaccac ggcgacaccc tcgccaccgc cacgccgctc gactcggtga
     4261 tcgacgtgat cggccacatc gggacccgcg ccgacgtcga cgccttctcc ttcaccaccg
     4321 caggcggggc cctcagcctc acggcgtcac cggtcgccgt cggcgccaac ctcgacatcg
     4381 ggctccggct cgtggacgac agcggcgccg tcgtcgcgag cgtcgaccca gccggcctcg
     4441 gggcgtcgct cgatgcgacg gtcccgggtg ggacctatgc cgtggtcgtg gacggggtcg
     4501 gtgtgggtga tccccggacc accgggtaca gcgactacgc ctcggtcggc cgctaccggc
     4561 tcaccggcgc gctccccgac atcgccggtg acggatcgga ccctgacccc gatgaaccgg
     4621 acccggagcc ggagttggcg gccccgtcca cgccgtcgct gaccgccacc gaggccgacg
     4681 gctcggtcac cctcgactgg ggggacgacc ccaccgcggc accgaccttc gcggtgtacc
     4741 gggagagccg gcacaagaac ggctcgtacc ggggtcggac gctgctcggc gaaggcaccg
     4801 aatccacttg ggtcgacacc cccggctccg gcaccttccg gtaccaggtc gtggccacga
     4861 actcctcggg ttcggccgag tccgacctca tcgaggtcac ggtgacctcg accacctccg
     4921 acggcccgac caagggcggc aagggcggtg gcggcggcaa gtccggcgcc atgaagaccc
     4981 gctgaacgcg acggtcgcag atcctctcgt catccagtgt gaggggtcgg cggcaacgtg
     5041 atgccggcgg cagacgcggg cgaccgccgg cccggaggcc ggcggtcgcc gcaggttcgc
     5101 tcggatgtga ggtggcgtca gggccggcag gtccagggag accatccctg cgtgttcacg
     5161 atgtgccggg catacagggc gttccagtac gggtcgtaga ccttgtccca ggactgaccc
     5221 gtgacccggg tgaagctggc ctcgtgccag cgacggttga tctggaacag cccgtggtcg
     5281 ttggtggggc tcaccgcggt cgggttccag ccggactcgc aggacacgac ccgctgggcc
     5341 tgggcgtgga gctcgccgga gccgccgttg tcggcgaagt gcatccagac ggcttcctgc
     5401 ggcgtgcacg cggcgccgaa gagcagcgcg atgccgaaga cggccagaag cgggaggcga
     5461 cgcgcgcggc gacgcagggt gggagacgtg gtcacgtgga ccctcctggg gggagttgga
     5521 gacggggacg ctgcaccaca tgacggtccc gtgtcgggat ccgtcctggc gcggcgcgcc
     5581 tgtccaggcg ttgcttggga agccaccttt ctatctggat ctcgcaacag aaccgcaaca
     5641 ttgggcctgt gaccttggtc gccccggacg cgcaccggcg tcgccgtctg cgggcccgtc
     5701 cgagatcaga tcgtcacatg caccccggtc tggtccgctc gcgcgctggg gtacggacgc
     5761 ccgcatggag atcatgggac gcttccgcaa ccgcaccgag accgtcgagc ggacgacctc
     5821 ggacgacgca cacttcgtgc tgatcgagac cgacgaccac gagcagatcg tgctcgccgg
     5881 ttcgagcggc gacgagatgc aggccaagct cgacgacttc ctcacctggg cctcctgagc
     5941 tcgctgcgga cccgatgagc atcgaggtcc gccaggccga catcacgacg ctggcggtcg
     6001 acgccatcgt gaacgcggcc aacgagtccc tgctcggcgg gggcggcgtc gacggcgcca
     6061 tccaccgagc agccggtccc ggcctcctgg ccgagtgccg gaccctgggt ggctgcccca
     6121 ccggggatgc caaggtcacc agcggccacg acctccccgc ccgctgggtg atccacaccg
     6181 tcggccccgt ctggcgtggc ggcgaccacg acgaagccga gctcctggcg agctgctacc
     6241 accggagcat ggccgaggcc gcccggctcg gtccggcctc gatcgccttc ccggccatca
     6301 gcaccggggt ctacggcttc ccgccagatc gagcagcacg catcgccgtc gacgcgatcc
     6361 gggcggagct ggacctcgac gggcgtccga ccgaggtcgt cctctgcgcc ttcagcgccg
     6421 acgccgccga tcttctggag gacgctctgc ggctctgagg cgtcggggtg cttggaggca
     6481 gcggcgggaa tcgaaccgcg tacagggatt ttgcagtccc ttgtccgaag acactcggtc
     6541 gggcaaggcc agtggccgtt ggaggcagcg gcgggaatcg aacccgcgta cagggatttg
     6601 cagtcccttg cctaagccac tcggccacgc tgccaggggg caggacccta gcggaacccc
     6661 cacgcggccc cgcatcgtca ccgcccgtga gcggggtccg gggtgggccc gccgatagcg
     6721 tcaccgggat ggctcgccag ccggccccgc cgggtccgcc gccgccgcac taccggcctc
     6781 cgggctcgcc gcgcccgctg cccggtgcgc tcaccccggc gcccgcgccg cgccccgcgc
     6841 cctcgggcgt gagcccgctg gccgccaccg cggtcgcggt gctgaccctg ctcgtcggcc
     6901 tcgtcgccgg cttcttcctc gggcgggcct acgacgacgg cgcgtccgtc tccgccacca
     6961 gcaccggcac gatcgcgccg tcacccacca ctccgacgtc gccacccgcc accccacccg
     7021 gtgacacgat cccccagcaa cccccgggcg ccccgcccgc caccgacctc gcgccgggga
     7081 acctcggcga gttcgacgag ccgatcccgg ccggccaggc ctacgtgctc ggcctgtacg
     7141 agatcgaggt cctcggggtc acgcgagacg ccggcgacgc cctcgccgag ttcgcccccg
     7201 ccaacccccc gccaccgtcg ggccagcagc acgtcctcgt ggagctcgcc gtccggttca
     7261 ccgacccgac cggtctcggc agcccggggt cgatcccgtt cttcgtgtcc gacggcgacg
     7321 gcgagtgggc cagcttcgag gccgggtgtg ggctcgtccc gggcgacctc cgggacacgg
     7381 ggttcatcga ggtgggcgac gaggccgtcg gtcaggtgtg cttcaccgtc ccgagcgacg
     7441 ccgtcgacga gctggcgctc ggcaccgaga gcttcgccgg ccccctctgg ttcgccttgc
     7501 cctgacccgc tcagtgcagg tcgtcgagcg cggcgaggac ctcctcctgg cgcgccgcgg
     7561 tggcccgcac catctcgggg tgggtgaaga tccagaaccg gtcggcctgg atggcggcga
     7621 acacggcatc ggccaccgac gacgctggga gaccgctctc gatcacctgg cggatcatgt
     7681 cccggagctc ctcggcccgg ggcgggcgct cgacggtcag ctccgccggt cggttccgct
     7741 cggactcgtg gatgcgggtg gacacccagc cggggcagag cacggagatg ccgaccgggg
     7801 tcccggcgag ctcggcgcgc atggactcgg acagggcgac cacgccgaac ttcgacaccg
     7861 cgtacggcgc catgccggcg gtggcgaaga acccggccat cgaggccgtg ttcaccacgt
     7921 gcgccggccc gccggactcg agcatgcgag ggaggaagct ggtgaccccg tggatgacgc
     7981 cccagaggtt caccccgagg acccactccc agtctttgcg ggacagctcg gccaccgtcc
     8041 cgcctccccc gacgccggcg ttgttgcaca gcagctccac gctgccgaag cggtcgtagg
     8101 cgcgtgcggc gaggtcctcc acggcgtcgg ggtcggagac gtcgcagcac gcggtcagca
     8161 cgccaccgtc gggggcaccg tcgagcgagc ccgctgccga ttcgaggccg gcttcgtcga
     8221 cgtcggcgag cacgacgttc atgccgtcgg cggcggcccg ctgggccagc gcgaggccga
     8281 tcccgctggc ggcgccggtg atgacggcgg tggttcctgg agctgtctgc attgctctcc
     8341 gatcaggtgg gaagtgccaa gattggccga tgcagatcgc ggtcgtctac gccagcagaa
     8401 ccgggaacac gcgccgcgcg gccgagctcg tcgggaccgc catcgccgag cgcgggcaca
     8461 cggtctcggt cgcctccgtg gacgacctcg actacaaggt gctcgccgac gccgacctcg
     8521 tcgtgctcgg cacctgggtg gcgggtcact tcgtgatcgg ccagaagcca ggcgacgccc
     8581 gccggctggc caaggtgccc aagctcaccg gcaagccggt ggccatcttc ctcacctacg
     8641 cgatcaaccc ggggtcggcc ctcggcaagc tggagaaggc gctgacccgc gagggtgccg
     8701 acgtcatcgg tggctacgcg tggaagcgga gcaacctgcc ggccggggtc gaggacttcg
     8761 tcgacggcgt gctcggcgcg gtcaagcccg cctcggcgtg agcgcgccgg gctggttcga
     8821 ccgggccctg gcgcacaccc ccgagacccg acaggtccag gtcgacggcg tcggcatcag
     8881 ctacctggcc tggggccggg tggccgctcc cggtctcgtc ttcatccacg gcggtgcggc
     8941 ccacgcccac tggtggagct tcctcgtccc gctgctggcc gactcctacc ggatcgcagc
     9001 catggacctg tcgggccacg gcgacagcga ccgacgcacc gagtacccca tgtccggctg
     9061 ggccgccgag gtcatggccg tcgccgacga cctcggtgcg gccggtgcac cggtggtggt
     9121 cggccactcg atgggcggct tcgtggccat cgccaccggg gccacctacg gcgaggacct
     9181 ggcgggcatc gtgatcctcg actccccggt gagcgcaccg gacccggaga ccgatgccgc
     9241 tcggggcggc aaggtgttcg gtccgctgcg cacctacccc gacctcgaca ccgcgctcgg
     9301 ccggttccgc acggtccccg agcagcagca ctacctgccc ttcgtgatgg accacgtggc
     9361 ccggcactcc ctccgagagg tcgacggcgg ctggtcctgg aagtacgacc cggagatctt
     9421 cgtgccgacc cgggccgagt cccgcgagct gctggcccgg gtgagctgcc gggtcgcgct
     9481 gttccgggcc gagcacgggc tggtgacgcc cgagatcggc cagtacatgt acgaccagct
     9541 cggacgggtg gcgcccgtcg tcgagatccc cgaggcgggt caccacctca tgctggacca
     9601 gcccctgctc ctgctcacgg cgctgcgcac gctgctcgcg gactgggagc actcctcgcc
     9661 ccgcacgcgc tgaggcgcgc tactccagcc gtcggcggca ccgcacgacg aggcgctggc
     9721 cgggtgccag gcgcacctcc atgctgtcga cgagggcctc ggtgatcacg agaccgcgcc
     9781 cgccggcgcg cagcgggtcg tcgaggtccc accgggcgac cggctcctcc gtcgcgtccg
     9841 tgcggttcac caggtccagg accagctcgg agccctcgca ccacgctcgc cctgtgacct
     9901 cctccgcgcc gtccggcgac gcgtcgacgg cgttggcggc gagctcgctg aacacgatct
     9961 cgagctcatc gaccgtctcg ccctgggtcc ggcgccgcag ccacgcagag aaggccgtgc
    10021 gggcccgggc cagggcatcc gtgcggggcg ggaaggacgt ctggaaggcg tcctcggtgg
    10081 gggagctcgg ctggctggcg gtcatcggcg ttccgttcgg tcgagcccac ctcgaacttc
    10141 cggtacggcc gactcgtacc cccggctccc ggtgcgcagt acaaccggca cgacccggac
    10201 agaagtcacg tccgggcgca aaacgaagcg gtgcccaggc caaggcctgg gcaccgtcac
    10261 gagcgggcga ccgggatcga accggcgacc tcaaccttgg caaggttgcg ctctagccag
    10321 ctgagctacg cccgcaggga ccggtcacca tagcaaggcg accgggtcgg gtggaatcga
    10381 ggtcaggtgc cccgctcgtc ctcgggccgg aggtcgacgg tgagggtgag gatgccgtcc
    10441 tcgaggtggc cctcggcatc ggcgatgatc gcgtagtcca tgaagtcggt cttggcctgc
    10501 tcgatgaagc gcgtgcgctc ctcccccgcc acgggtggca ggttgcgctt cttcaccgcc
    10561 gccgcccgct ccttgaagcg ggcgagcatc gcatcgacgt cgaggttgtc ggccatggcc
    10621 atgaacctac cgacgggtca accccggtgg aacacggtgg tgtcgtcggg gccctcatcg
    10681 ggggtcgccg ggcgggttcg cacccagtag cgccaggtcc acacgccgat gagcagggcg
    10741 acgaggacga gccccgccac gatgagccag atctgggcct gctcgtcgag cgccccgtcc
    10801 tcgtcagcag cgacctcgac gaccgtggtg gaggaggtgg tggtcgcctg cgctccgtcg
    10861 gagccgtccc cgctcaccag caggtcccgc gccgtgctgg tggtctcggc cggttcgtcg
    10921 tcggcgacgg ttccggcgtc ctcggccacg tcctcctcgg tgacgacgcc gtcgtccccg
    10981 tcgtcctcgt cgtcagaccc ggccgtggtg gtcggccgag cggtcgtgcc ggtgacccgg
    11041 gtcgtggtgg tcgtctcctc cggctcgggt tctgcctccg cctccgcctc ggtcgtcgtg
    11101 gtctcgaccg gcgcctcggt ggtcgtcgtc gccggggcgg tggtgctggt cggcaggatc
    11161 tggccggcgg cgccggacgc ccccgggccg aggatcgccg cgctcgccgc cagcaggagg
    11221 gagagcgcgc gccgcagggc gacgcgccgc cgggggtggg gctccgctgc gggcatcgtc
    11281 ggacgaaggt actgcaggtg ctcgccgccg ccccaggcgg ggatcaccgc tggtggcggg
    11341 ggcagcggta ggtggtgcgg ccaccgacgg tggcgcgatc gagaacggcg ccgtccctcg
    11401 ggcacgtggc ccccgggacc cgggccggct gcaggtcgcc ggtgtggctg ccgccacgct
    11461 gcatgagctc ggcgacggtg gtgcggatgg cctcggccag cagcgggacc tcgtcgtcgg
    11521 cgatctcgtc gcacgggcga gccggatcga tgccggcccg ccacagggtc tcgtcgacca
    11581 gcaggttgcc cagcccggcg agacgggcct ggtcgagcag tcgggccttg atcgccgtgc
    11641 gcgacccggc cagggccgtg cgcagctgtg ccgccgtgac cgtgctcgcc tcggggccga
    11701 ggcggctggt gtcggggtcg agctgcagcc cgccgagccg gcgggggtca cggatgcgga
    11761 ggtcggtgcc gtcgctgaac gtgagcacgt agcggtccca cgccgggtcg tcgccctggc
    11821 cgccgtagag caggccgtcg atggtcgcgc caccgtccac cagcagccgg ccggtcatgc
    11881 cgaagcggag cccgaggacg ggaccgtcgg tggggagcag gagcagcttg ccgatccgct
    11941 cggccccctc gatccggtgg cccaccagct cggcccgcac gacgtcgggg tcggcgccgc
    12001 ggaggccgag cgggtcgagg accggggcgt cggtgatggt gcgaccgacc accgcctcgg
    12061 cctggcggcg gtacgtctcg acctcgatca gctccggcac gggcagccgg cgatcagagg
    12121 gcggcgagca cgtcggcgag cacgtcatcg acgtgctcga gcccgacgga gatgcgcagc
    12181 atcccctcgg ggatccccat ggcgacgcgc tcgtcgggcg acagcgacgc cgccgacatc
    12241 gatgccggat gggtcacgag cgtctcgggt ccgccgagcg acaccgccgg gatggccagc
    12301 tcgatccgct cgacgaaggc ccggcccgcc tcgtagccgc cggccaggtc gaccacgacg
    12361 atggagccgc cgctgcgcat ctggcgcatc gccagctcgt gctgggggtg ggacgccaga
    12421 ccggggtacc ggacccgctc gaccgcgggg tgcgcgtcca ggcgctcggc cagcacgtgc
    12481 gcggtctcgc acgcctggcg ctggcggacg tgcagggtgc gcaggccccg caggccgagg
    12541 aacgcgtcga agggcgaggc gaccgcgccg tgcaccacgt ggtgggccca caggacgtcg
    12601 atcagctccc gctcaccggt caccaccccg aggagggcgt cgttgtgacc cgcgatggcc
    12661 ttggtggccg agtggaacac caggtgcacg ccgtggtcgt gggggttctg caccgccggt
    12721 gtggccatgg tggagtccac gaccgtgaac ggacccatga gcccgccgac ggcctcgagg
    12781 tcgacgatgc ccatcgccgg gttcgccgga gtctcgcacc acaccagctg ggtcggcttc
    12841 tcggccacgg cggcgcgcag ggcgtcggcg tcgaacgcgt cgaccagggc gacctcgatc
    12901 ccgaaccgtt cgcacgtgcg ggtcagcagc tggttggtga ccgagaacat ggatgcctgg
    12961 gccacgatgc ggtcgcccgg ggagcacagg gcgaacacga cgctcgacgc cgcccccatc
    13021 cccgatccga acgcgatgcc ggcctcggcg ccctcgacct cggccacggc gtcggcgaag
    13081 gcctggaccg tgggcgtgcc gttccgggcg tagaactcgg tggcccgggg cgtcacggcc
    13141 atgcgggcgc tcacgtcgag gtcgcgcagc tcgtaggccg tggacggcca gatggggctg
    13201 gcgaccgagc cctggttggg gccgcggccg gcgcggatgg cgctggtctc gcggtgggcg
    13261 tcgtcgctca cgtggtgctc ctgtgggagg tggcggtcgg gtcgtcgagg tggggggcga
    13321 gcacgtcgcg catctcctcg tgctcgatga ggaacgcgtc gtggccgtgc gggctctcga
    13381 ggcggtggta gtgggccggg agaccggcgg cacgggccag cgcggcgatc tcggcggcct
    13441 ggtgggccgg gtagaggatg tcgctgctga tgccgaccgt gacgacgggg caccggacgc
    13501 gcgccatggc cgcgtcgatg ccgccccgac cccgtccgat gtcgtggagg tccatggcct
    13561 tggtcaggac caggtagctg ttggcgtcga agcgccgggc cagcttgtcg ccgtggtgct
    13621 ccaggtagcg ctcgacctgg aagcgctgcc acaggtggaa cccgtcgagg ggctcgacca
    13681 gctcccggcc gaagcggtcg gtgaacacgt cgtcggaccg gaacgtgatc tgggacacca
    13741 tccgggccag ggccagcccc tcgtgcggac cgtcgcccgg agcggcgtcg tagtagtccc
    13801 cgttccgcca cgccgggtcc atgcggatga tccggcgccc ggtcgaccac cacccgatct
    13861 gctgggccga ggctgccgcg gtggaggcga ccacgatcag ggagccgacg cgctcggggt
    13921 gcatgatcgc ccactcgagg gcctgcatcc cgcccatcga gccgccgatc accgcgtgcc
    13981 accggcggat gccgagctcg tcggcgacga cccgctgggt gcggaccatg tcgcgcaccg
    14041 acacgaccgg gaagcgggca ccgtaggggc ggccgtcgtc ggggtgggga gacgccggcc
    14101 ccgtcgagcc ctggcagccg ccgagcacgt tgacgcacac cacgaagaag cggtcgaggt
    14161 cgatgcccga cccgtgtcgg accatgccgt cccaccaccc cggtgagagg tgaccagggc
    14221 cggccgcacc ggccacgtgg ctgtcgccgg tcagggcgtg gcagacgagc acggcgttgg
    14281 aggcgtcggc gtccagggtc ccgaaggtct cgtaggcgac caccgcaccc tccagctggc
    14341 cgccgccctc cagggcgaag ggccgacccg gcgtgatcgg cgcgaaccgt cggttgccga
    14401 tctcgtcacc gtcctgccag gcgccggagg cgggcagggt gctggggtgc gcccggcgac
    14461 cgaggtcggc cagggggcgg tcggggtcga ccacaccgga gagggggccg ccacgagcgc
    14521 cgttcgcgga ggaggtggtg gtgcgctcgt cgcgcacctc gccgtcggtc gtcatgtggt
    14581 ccacctccct ccggagggcg gacaccctgt gacgcttcgg gacggacccg gacgcttggt
    14641 tgccccggac ggggccgcat ccccacccgg aggagggagg caccttgggt gtccgttccc
    14701 aggttgccgg acccggtctc ccgggccgct cgtgatgctc gtgcgggccg gagtgtaggg
    14761 ggacgaccga gcccaggcca caaccggttg tccggagctc agcgccgggg gcggcgggcg
    14821 tcccaccagg cgagcagatc ggggtcgtcg accccgaggg cggcgtcggc caggcgagcg
    14881 acctcggtga cgtcggcgtc gcccacgctc gccgcctggg ctgcgtcggc agccaggtcc
    14941 caggccggcc cgtcctcggg caccggcagc gccgagagca gggcgacggt gggacggcag
    15001 gcatcggcgg acagggcggt gccggcgctg cgccggaaca gccatgccgt cgcggccggg
    15061 gcgtggaggg cggcgaggag gtgccacagc gcatcggcgt cgtgggggcg cacgcacagc
    15121 accggcacgg acccgagcag cgagccgtcg ggatcggcca ccgcctcgag cacccgggtc
    15181 tgggtggcca cgagcagctt ggggcccgac tgcaccgcgg cccaccgggc cgtgcgcccc
    15241 tggagcgacg acgtgtgcac caccggggcg gtccagcgcg tcccggcgaa gcgctgaccc
    15301 cgggcgagca ggcggcacgg gtcgatgccg ccggccgtca ccaggcgggg ccgggcctca
    15361 ccgccatcgg cctcggactc ggacacggcg tcgacgagcc cgtagtactc gtcccggaac
    15421 ccggcgtgca cggtcgccag gctgccgagg cggcggtccg aggtcagcgc gaccgacggg
    15481 atgccggccg cgtcggcgag cggggcgacc cagtcgttgg gctccacacg cgagccctcg
    15541 ggccggcgcc ggaagaccgg cgcgcagacc tgcacgttgg cggcgaaggc gccgccgtcg
    15601 tcgacgacgg tgtgcaccag gtcggcgcag gcatcgatgc ggtcccgcac cgcggcggcg
    15661 tcgcgggcgg ccaggaacga ctggggctgg acgagcgcca cgtggccgcc gggggcgacc
    15721 tgctcgaccg cggtcagagc gaagagccac gccgggtcgg tgtaggcccg gacggcgtcg
    15781 ccgaagcggg cccggagcgc cgctcggcga tcggcatccg cggatgtcga cgaccggagc
    15841 tggccgagga agggtgggtt gcccaccacc gatccggccg ggggcagcgc cacctcggtc
    15901 agggcatcgc cgacgacgag ggactccccc atccggatcg gacaccccgc acgggccgac
    15961 caccaccaca gggcggcctc gcacaccgcc acggccagcg gatcgacgtc gcagccccag
    16021 agccggccga ccgcatccgt cggatccagc cccccggcga cgaggtggtc ggcggcggcg
    16081 agcaggaaga ccccgccgcc gcaggacggg tcgaccacgg tgccgtcggc gacgacggcg
    16141 cgccggacga ggtcctcggc cagccaccgc ggcgtgtacc aggcgccccg ccgggtccgc
    16201 tcggtgccgc cgacgaggcg ctcgtgcagc gggccgaggt cgtgcggatc gtcgaccgcg
    16261 agatcgccgt cgagcgggac gggaccgtgc agcagccgca gggcgggatc cgggacgaag
    16321 ccctcccggc gggtggccgt ggcgaaggcg ccggcgaggg tcgcagcgac cgacggcacc
    16381 gtcgcgcccc gctcagccgg cgaaggggtc ccagccggcg aggtccttga ggcgctcgtc
    16441 gttggagaac atctcgacca tggggtgctc gtcgaggccg aggcgacgct gcaggagctt
    16501 caccgacatc tcctcgtccc acttgatgag cgtcaccgcg acacccgtgg tgccggcgcg
    16561 ggcggtgcgg cccgagcggt gcaggtaggt cttgtggtcc ggcggcggat cccagtggat
    16621 gaccgtgtcg atgaggtcga tgtccaggcc gcgggcggcg acgtcggagg ccacgagggc
    16681 ttggatcttg ccgtcggtga accgctggag cgccttctcc cgggcccgct ggggcaggtc
    16741 gccgtggatg ggcgacgcct tcacgccgag ctcgtcgagg tcgtcggcca gccggtcggc
    16801 ggcgcgcttg gtgttggaga agatcagcgt gcggttgccg ttctcgacga tggccgccac
    16861 gaccttggcc ttgtcccgct cgtggatctt gatgaagcgg tggtgcatct ggtcgacggt
    16921 gaccgtctcg gagacgacct ccttggtgac cgggtccgtc tggtagcggt cgatcagcgc
    16981 gccgaccgag ccgtcgaggg tggcggagaa cagcagggtc tggtggccct cgggcacgtg
    17041 gcgcagcacc cactcgacct ggggcaggaa gcccatgtcg gccatgcggt cggcctcgtc
    17101 gacggcgacc atcttgacct cgtcgagctt cacgtcgccg cgctggagca ggtcgatgag
    17161 ccggcccggc gtggccacga tgatccccgt gccgtcgtcg atggacttga tctgcttctc
    17221 gatcttctcg ccgccgtaca ccgcggtgat ggtgtggccc ttgaccttgg ccagcggctc
    17281 gagcacctcg gcgacctgga gcgcgagctc gcgggtgggg cacaggacca ggccggccgg
    17341 gcccttgtgg atgtcgtcgg gctcggggag cgccatgacc atcgggacgc cgaaggcgag
    17401 ggtcttgccc gagccggtct tggccttgcc gcacacgtcg cggccggcga cggcgtcggg
    17461 gatggtgagc tcttggatgg ggaagggcga ttcgatgccc tgggaggcga ggcactcgac
    17521 caggtcgtcg tcgatgccca gttctgcgaa agtcgtcatg ttggcgacaa cggtaccgtc
    17581 agatcccgcg ggcctcgcga tcgccggtac cgtccgcgtg tgcgcgagca tctgacgaaa
    17641 gcccaggtgg aggccttggt ggcccggctc gatggacctc ccgacgccct ggccgaggcg
    17701 ctcgccgatg ccgtcgaggc cctgcacggc cagcgagacg tggcgggcgt cctcgactcc
    17761 ctcggccggc accacgacgc gaccggcgtg cgcacgcgcg acgaggccac gctgtgggcg
    17821 ctcgccaccg agctcaacga gcgccgctac ctctgaccgt cgcccccgca cctcgtccca
    17881 cgagaactgc aagacttcgc tggtggaact cgcgcacgac ctcgggggga gggacggctt
    17941 cggtgcgccg atcgtggagg aggacgaacc ggtgttccac gcggcctggg agcgcaccgc
    18001 acgcgccctc gtcttcgccg ccatgggcgc ccacccgaac ccgacgacct ccgcgttccg
    18061 ccatgccatc gagcggatgg accccgagca ctacctcgcc agcagctact acgagcactg
    18121 gctcacggct gcggcctcca tcgcggtcga gaccggcgtc gtcacccgcg aggagctcga
    18181 ggcgtcggcc ggcggcgcct tcccgctggc gcggcccgag gtgcgacccc acgtcgacgg
    18241 gagcggcggg cagcgcttcg ccgtcggcga ccgggtccgc gtcgtcgcgg tcgagcacgg
    18301 gggacacacc cggtgcccgg gctacctgcg cggccgggtc ggcaccgtga cccgggtcga
    18361 cgagcgcttc agcctgcccg acgtggaagc gcactcgacc cgccgcgtcg tggagggcac
    18421 ctacggcgtg cgcttctcgg cccgggacct ctgggccgac gccgaccagc acgcggccgt
    18481 gcacgtggac ctgtgggaca gctacctgga ggcgacatga cggccgacca ccaccatccg
    18541 accgagcccg cccccatcga gcggcgcgtg gcggcgctcg agcagctgct caccgagcgc
    18601 gggctggtga cccccggcct cgtcgacgag gtgaacaggg cctacgagga ggacatcggg
    18661 ccccgcaacg gcgcccaggt ggtggcccgc gcctgggtcg accccgagta caagcgccgc
    18721 ctgctcctcg acggcacggc ggccatcgcc gagctgggct tcggaggggc cgagggagac
    18781 cacatgcagg tcgtcgagaa caccgacgcc gtccacaacg tggtcgtctg cacgctctgc
    18841 tcgtgctatc cgtggccggt gctcggcctc cctcccgcct ggtacaagga cccgccctac
    18901 cgtgcccgga tggtgcgcga gccgcgcgtg ctgctcgccg agatgggatg ccaggtcgac
    18961 gacggcgtcg agatcgccgt gtgggactcg tcggccgagc agcgctacct cgtgctgccc
    19021 cagcgacccg ccggcaccga cgatctcgcc gaggatgacc tggctgcgct ggtcacccgc
    19081 gacgcgatga tcggcgtcga gcggctgtga gcggtctcga tcgcatcgag gtcccgcgac
    19141 gcaacggcga gctcgcattc gatgcgccct ggcaggcgcg ggtcttcggg ctcgccgccg
    19201 tgctggtcga gacccggttc gacggtgact gggaaccgtt ccgcagcgag ctcatcaccg
    19261 ccatcgccgc cgacgaggcc cgtccgtact gggagagctg gacggctgcc ctcgaaggcc
    19321 tcctcgtcgc agcccacctc ctctcggtcg acgacctgga tgcagccacc tcgtcgcgac
    19381 cctgaactca acgcggttca gcggcggcgc atgccccgca cccgccggtc gccgacgtcg
    19441 acaccctcgg ccacgagtcg acgtcgctgc tcggcctcct tgcccggggc gagccgaccg
    19501 tcggcggcga cgacccgcca ccacgccacg tcggcaccgc tcgtggccat cacccgcccg
    19561 acggcccggg cggcacccgg acggccggcc tcggcggcga cctcgccgta ggtgatcacc
    19621 tcgcccgccg gcagcgcggc gagcacagcg agcaccgcgt cctcgaacgt cgtggccacc
    19681 gcggttgcct accatcgacg ccgatgcgca cgctcaccga cgccgacgcc gatcgccacg
    19741 ccgccgagat cgaggagcgc ggctacacga tcgtcgagga cgccttcgac gccaccttcg
    19801 ccaccgaggt gctcgatgcc ctcgacgcgg tcatcgagca gcgccagctg accccggccg
    19861 acaacggctt cgagggccgt gacacgtacc gggtgtacaa cctgctcgcc cacgacccgc
    19921 tcttccagcg catcccgacc gcgccggcag tgctccccgt cgtcgagcgg gtgctggacc
    19981 gggagtgcct gctgtccacc ctgtcctcca tcgacatccg cccgggtgag tcggcccagc
    20041 ccatccactc cgacgaccag gtcatcggcc tgccccgcac cgggttcccg gtggtgtgca
    20101 actcgatgtg ggccatcacc gacttcaccg aggccaacgg cgcgacccgc ctggtcccgg
    20161 gctcgcaccg cgaccccgag tacccggtgt acggcgagac ctacgacacc atcccggccg
    20221 agatgcccgc cggcagcgtg ctgctctggc acggcagcct gtggcacggc ggcggtgcca
    20281 accgatccga cgagcgccgc gtcggcatcg ccgacaacta ctgcgccggg atgatccgcc
    20341 agcaggagaa ccagcagctc ggcgtgcccc ggcacatcgc cgccacgttc gacaagcgcc
    20401 tcgcccgcct gtgcggctac ggcacgtacc acgggctcat cggccacatc gacaagaccg
    20461 acccgctccg cctgctcgac cgggacgacg ccgacgacac catgctctgg gacgccggct
    20521 gagcgtggcc gccctcgacc agatcgaccg ctgggacgtc cccaccgtcg ccgccggcgt
    20581 cctcgggccc gacggcctcc tcgccggccg cggcgagacg gaccaggcct ttccgctggc
    20641 gtcggtgagc aaggtgctgg ccgccatcgc cgtccacgtc gcggtcgagg agggcacggt
    20701 cggcctcgac gacgacgtca ccgacgccgg cgggccggag ggcgcgacgg tgcgccacct
    20761 gctcgcccac gccagcggcc tgccgccgga cggcgaccgc acaccgctcg gcccacccga
    20821 gcgcaagcgg atctactcca acgtcggctt cgaggtcctc ggcgaccacg tggcgagccg
    20881 caccggcatg gcgatgaccg actacgtgcg cgccgccctg gtcgacccgc tcggactcgg
    20941 tgccacgacc atcgacggct cgccggccca cggctacgcg tcgtcggtcg acgacctcgc
    21001 gtcgatcctc gtcgccctgc acggcgggcg cctgctcgcg ccgagctcca tcgaggccat
    21061 gacgaccccg gcgttccccg acctcgccgg cgtgctcccc ggctacggcg cccaggaccc
    21121 gaacccgtgg gggctcggcg tggaggtgcg cggcaccaag gacccgcact ggaccgcgcc
    21181 cgccaacagc cccgagacgt ggggccactt cggccggtcc ggcacgttcc tgtggtggga
    21241 ccccaccgtc ggggtgggcc tcgtcgtgct cgccgaccgc gagttcggtc agtgggccat
    21301 cgatgcctgg ccgcagctgg ccgccgacgt ggtcgccgaa cacggctgac ccgaccggtc
    21361 acggcagctc gcgcgccacc cagtcggcca gcagcgggtc ggtgagcgac gacccgccca
    21421 gcccgaagag caggtggccg gcgtcgacga gcgagtcgcg tgcgatcgtg atcgacgagc
    21481 gcgacaggtc gtggaaccgg ccctccgagg cggcgaaggg tgcacccgtg cgcaccacgg
    21541 cgcgcagcac cgcctgctgg gtgctggtga gcatgccgaa gaagtcccgc atgcccgggg
    21601 cgacgcggga gcgcacgccg gccagggcct cgtcccacgt gtcgggaccg gcgatcccgc
    21661 ccggctcggt gagccaccac gcggcatcgg ccagctccat ggagcgctgc gggtggccgg
    21721 caccgaacgc ggcgatcgag gacgccaccg gtccggcgtc ccgaccggtc gagcggaacc
    21781 cccggtggac gatgtcgacc acggcctcga ccgggagcgg cagcagctcg atgcggcggg
    21841 cctgggagta gaagggctgg tcggtgtcgc cgaagaggcg ggccatcatc gtgggcttgg
    21901 agccggcgaa ggcgaggccg agctcgcggt agacgggctg caggtgggag cgcatgagct
    21961 cgagcacgtt cccgacgccg atggcgtcgg cgacctcgtc gatgacgagg acggtgcggt
    22021 ggtgctcggc cacccggacc agcgactcga gcagggcgtg cacggtggcc gtggcgtcgg
    22081 gcgccgagcg ggccgaccgg cgcagctcgg ccgagacgac cccgatgctg atgcgcagac
    22141 cgcccgcgac gcgatcgagc gcctcgcgga accggccggt gcaggcgccg agggcccggt
    22201 cgagccgcac ggcgaagtcc gagaccgagg acagcccgta gaggtcgagc cacacggtgg
    22261 tggtcggctc gacctggtcg aggagccacg cgccccgccg cagcaggctg gtcttgccgt
    22321 accgccgggg cgcgacgagg gcgatgggca cgcggccgcc gaggaggcgg accagctcgg
    22381 ccgcctcgtc gtcgcggccg gtgacctcat cggcctccag cgggccggag tgcgggaagg
    22441 gggatcgctc cacgtacgga acggtacccc ggaccccctg acggttaccg aggtctggga
    22501 gcgaatcgtt accgaactac ggtaacgatt cgctcccgat ccacgggatc agaactgctg
    22561 gggcgtcagc gccatgaggt cgccgaaccc ggcggtgacc gcgccggtga ggccaccggc
    22621 ctgcgggagc agctgggtgg cgtagaagcg gacggtcgtc tgcttggcct cgaggaactc
    22681 ggagctgaag ccgccaccgt cgccggcggc gagctcggca gcgtccagcg cggactcggc
    22741 cagcagccag ccgccggcga cgatgccgaa ctggcgcatg tacggcgtgg cgccggcgag
    22801 ggcgtggcgc acgtcctcga gcccgttggc catgagccag tcagtgctct cggtgagcgc
    22861 ggccagcgcg tcggcgagcg catcggcgat gacggcgtgg tcgcctccgg cggcccgacc
    22921 cttctcgacc gtggcgccca cgtcggcgag gaagtccttc accacgcccc cggcgcgcat
    22981 cggcagcttg cgcccgacca ggtccatcgc ctggatgccg ttggtgccct cgtagatcgg
    23041 ggcgatgcgg gcgtcgcgga agtgctgggc gacgccggtc tcctcgacgt agcccatgcc
    23101 gccgaagacc tggatggcgg tggaggtgat gtcgacgccg aggtcggtgc cccagccctt
    23161 gcagaccggg atgagcaggt cgacgcgctc ctgggcggtg gtgcgggtct gctcgtcctc
    23221 gtggcggtcc gcccggtcga tgccggcagc cgtgtagtag atgacggcgc gcaccgcctc
    23281 gagctgggcc ttcatcgtga gcagcatgcg acgcacgtcg gggtgctcga tgatgaggct
    23341 ctgctccccc gccggcgcgc cgatggcccg gccctgctgg cgctcgttcg cgtactgcac
    23401 ggcgtcctgg taggcgcggt cgccgacggc caggccctgg aggccgaccg acaggcgggc
    23461 gttgttcatc atcgtgaaca tgtacgccat gccccggttc tcctcgccga cgatgtagcc
    23521 gacggagccg tcgtagtcga ggacgcaggt ggggctggcc ttgatgccga gcttgtgctc
    23581 gatggactgg cagaccacgc cgttgcggtc ggtgaggttg ccgtcgtcgt cgatgagcac
    23641 cttggggacg atgaacatcg agatgccctt ggtgccggga ggggcatcgg gcgtgcgggc
    23701 cagcacgagg tggacgatgt tgtcggccat gtcgtgctca ccccagctga tccagatctt
    23761 ggtgccggtg atgcggtagg tgccgtcgtc ctggcgctcg gcgcgcgtgc ggaccgcgcc
    23821 gacgtcggag ccggcctggg gctcggacag gttcatcgtg gcggtccact cgccgctcac
    23881 catcttgggc agccacgtct ccttgatggt ctcgtcaccg tgctccagca gcgcgtcgat
    23941 ggcgccctgg gacagcagcg gggccatgga gaacgcctcg ttggcagcgg tcatcatctc
    24001 ctgcatggcg atgcccacca gccaggggaa gccgccgccg ccgaactcct cggggaacgg
    24061 gaccccgccc cagccggcct tcacgtagtc cttgtaggcg tcgatgaagc cggtgggcgt
    24121 ggtgacgctg ttgtcctcgg cgtgccacac gctggtctcg atgtcgccga cccggttgag
    24181 cggggcgacc ttgtcctcca tgaaccgggc acactcctcg aggatgctgt gcaccatctc
    24241 cggctcggcg tggccgaacc cctcgaggga ggacagctcc cccagcttgg cgatgcggtc
    24301 cagcgcgaag cggatgtcgt cgaggggtgc ggtgtagtca gccatggcat ccgagcctac
    24361 cgaggggtaa ccgaaggctc cgtggcaggc tgctcctgcc catgggaccg accgacgcca
    24421 tgcccccgac cgtgcaccgc gcccggttcg ccgacctctc cccccaggac ctctacggca
    24481 tcctgcgcct ccgctccgac gtgttcgtcg tcgagcagga ctgcgtgttc ctcgacctcg
    24541 acgagcggga ccacgaggcc gacgccgagc acctgtggac caccgacggg tcgggggtgg
    24601 tggccacgtt gcgcctgctg cacgagggtg acggcacgtg gtcgatcggc cgggtcgtgg
    24661 cgcgggccga cgctcgcagc cgcggggtcg ccgcccacct gctgcaggcc ggcctcgatc
    24721 ggctgcggga gctcggcgcg gagaccgtcc gcatcggcgc ccaggcccag ctgcgcgact
    24781 ggtacgcccg cttcgggttc gcccagtccg gcccccgcta cctcgaggac ggcatcctcc
    24841 acctgccgat gtcgctcagc ctcggcgcgg accgatgagc ctcgtcacgc tcgacagcgg
    24901 gatcctcgcc tggctcgccg aggacccggg cgacggccgg cccaacgccg gcctcgtcgt
    24961 cgacgcggac gccgccaccg ccatcgacac cctctgcgtg ccgtcccaga ccgccgcgtt
    25021 cgtcgccgcc atcgacgtgc tcggcctccg ggtgcggcgc accgtcctca ccgcatcgac
    25081 gatcgagttc gtcggcggta cggcgatgtt caccatgtcc ggcatgtacg ggcgccgcca
    25141 gaccagcgtc cacctcgacc agccaccgac gccggacatc taccgccggc tgctccccga
    25201 cgtggccgac gagttcgacg acgagctgac gaccaagccg gtcagccaca tcgtcgccga
    25261 ccgcgtgcag ctcacccccg ccatcagcct gcacccgtgc tcgggcgccc aggaggagaa
    25321 cctcgtcgcc gtggtcgacg
//
LOCUS       DATISU010000423         3108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_735706, whole genome shotgun sequence.
ACCESSION   DATISU010000423 DATISU010000000
VERSION     DATISU010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735706"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..970
                     /gene="fabD"
                     /locus_tag="VLR27_16860"
     CDS             47..970
                     /gene="fabD"
                     /locus_tag="VLR27_16860"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HSP05178.1"
                     /translation="MLTFVFPGQGSQQPGMGAAFADDAAWSVVEQASDATGRDVAHLL
                     LDAAADELVETRNAQLGTFVHSMVVLEALRSRGVQAQAVAGHSLGELSALCAAGVLGF
                     DDAVRLVAERGEAMQVCCTQRTGTMAAVLGLDEPQVIEVCAAVDGDVWVANSNAPGQV
                     VIAGDPAAIDAATIVAKEAGAKRVMPLDVSGAFHTPFMAPAGERLAAAIADATFHDAT
                     TPVAANVDGAIHTDAGEWPALLERQLTSPVRWTGCVESLLAAGATTFVEIGPGAVLTG
                     TIKRIDKDSARHSVMTPDQLEAVVTALGADA"
     gene            967..1971
                     /locus_tag="VLR27_16865"
     CDS             967..1971
                     /locus_tag="VLR27_16865"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HSP05179.1"
                     /translation="MSDRTEAGNAVTGGLRGGRIIGWGTALPDQVLSNADLEANLDTT
                     DAWITERTGIKERRYGGTTAGLAAEAGRKAIESAGVDPASIDLLILCTTSPDRNLPAS
                     ASTVQAQLGLDCGAFDLNAACSGFVYGLAAANGYIAMGTKRILLIGAETLDKMTDPED
                     RGTAILFGNGGGAVVIDSVPEGEPAQLLGWDVGSDGDAEHILYAELGGYMQMDGREVF
                     RKAVRVMVQSANAAMERAGVTAKDIALVVPHQANIRIIDSACDKLGFDREQTALVLQS
                     TGNTSAASIPLALSEALEQGRVRDGDLVLLVGFGAGMTWASAVLRWSEAGPVEIGAAP
                     "
     gene            1968..2678
                     /gene="fabG"
                     /locus_tag="VLR27_16870"
     CDS             1968..2678
                     /gene="fabG"
                     /locus_tag="VLR27_16870"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HSP05180.1"
                     /translation="MSTPRTVLVTGGNRGIGLACTQAFVADGHRVATVSRSGGEVDGA
                     LAVACDIADPAAVDAAIATVEEQLGPIEVLVANAGMTKDGLLVRMSDDDFTSVIETNL
                     TGSFRFARAVTKNMMRARFGRIVFMSSVGAYMGAAGQANYAASKAGVIGLARSMAREL
                     ASRSVTVNVVTPGPIDTDMFAAVTDKRRDELAATVPMNRLGSPDEVAAVVRFLASDEA
                     SFVTGAVVPVDGGLGMGF"
     gene            2727..2960
                     /gene="acpP"
                     /locus_tag="VLR27_16875"
     CDS             2727..2960
                     /gene="acpP"
                     /locus_tag="VLR27_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HSP05181.1"
                     /translation="MSDDFERFQKCAVEVLSVEATQVTKEASFADDLDADSLDLVELV
                     MALEEEFDVTVEEEELEGIETVGAAFDLIKGKL"
BASE COUNT          468 a         1191 c         1028 g          421 t
ORIGIN      
        1 gcacccgcgt gaaacctggc gcgggtggtc gcggtcggac cgattcatgc tcaccttcgt
       61 cttccccggc cagggcagcc agcagcccgg catgggcgcg gcattcgctg acgacgccgc
      121 ctggtcggtg gtggagcagg catcggacgc caccgggcgc gatgtcgccc acctgctgct
      181 cgacgccgcc gccgacgagc tggtcgagac ccgcaacgcc cagctcggca cgttcgtcca
      241 ctcgatggtc gtgctcgagg ccctgcgcag ccgtggcgtc caggcacagg ccgtcgccgg
      301 acacagcctg ggtgagctct ccgccctctg cgccgccggc gtgctcggct tcgatgacgc
      361 tgtccggctg gtggccgagc ggggcgaggc catgcaggtc tgctgcaccc agcgcacggg
      421 caccatggcc gcggtcctcg gcctcgacga accgcaggtg atcgaggtgt gcgccgccgt
      481 cgacggcgat gtctgggtgg ccaacagcaa cgcgcccggc caggtcgtca tcgccggcga
      541 tcctgctgcc atcgacgccg cgacgatcgt ggccaaggaa gccggcgcca agcgggtcat
      601 gccactcgac gtctccggcg ccttccacac cccgttcatg gccccggccg gcgagcgcct
      661 cgccgccgcc atcgccgacg ccaccttcca cgacgccacg acccccgtgg ccgccaacgt
      721 cgacggcgcc atccacaccg acgccggcga gtggccggcg ctgctggagc gccagctcac
      781 cagcccggtc cgctggaccg gctgcgtcga gtcgctgctc gccgccggtg cgaccacgtt
      841 cgtcgagatc ggccccggcg ccgtgctcac cggcacgatc aagcgcatcg acaaggactc
      901 ggcccggcac tcggtgatga cgcccgacca gctcgaagcc gtcgtcaccg ccctcggggc
      961 ggatgcatga gcgaccgcac ggaagccggc aacgccgtca ccggcggcct gcgcggcggg
     1021 cggatcatcg gctggggcac ggccctaccc gaccaggtcc tctccaacgc cgacctcgag
     1081 gccaacctcg acaccaccga cgcgtggatc accgagcgca ccgggatcaa ggagcgccgc
     1141 tacgggggca ccaccgccgg cctcgccgcc gaagccggcc gcaaggccat cgagtccgcc
     1201 ggcgtcgacc ccgcatccat cgacctgctg atcctgtgca ccaccagccc ggaccggaac
     1261 ctgccggcgt cggccagcac ggtccaagcc cagctcggcc tcgactgcgg cgcgttcgac
     1321 ctcaacgcgg cgtgctcggg cttcgtctac ggcttggccg ccgccaacgg ctacatcgcc
     1381 atgggcacca agcgcatcct gctcatcggc gccgagacgc tcgacaagat gaccgacccc
     1441 gaggaccggg gcacggcgat cctgttcggc aacggcggcg gcgcggtcgt catcgattcc
     1501 gtccccgagg gtgaaccggc ccagctcctc ggctgggacg tcggctccga cggcgacgcc
     1561 gagcacatcc tctacgccga gctcggcggc tacatgcaga tggacggccg cgaggtgttc
     1621 cgcaaggcgg tccgcgtcat ggtccagtcc gccaacgccg ccatggagcg tgccggcgtg
     1681 accgcgaagg acatcgccct cgtcgtcccc caccaggcca acatccgcat catcgactcg
     1741 gcctgcgaca agctcggctt cgaccgcgag cagacggccc tcgtgctcca gtccaccggc
     1801 aacacctccg ccgcgtcgat ccccctcgcc ctctccgagg cgctcgaaca ggggcgtgtc
     1861 cgcgacggtg acctcgtcct gctcgtcggc ttcggcgccg ggatgacgtg ggcctcggcg
     1921 gtcctgcgct ggtccgaggc cggcccggtc gagatcggcg ccgccccatg agcacccccc
     1981 gcaccgtcct cgtcaccggc ggcaaccgcg gcatcgggct ggcctgcacc caggccttcg
     2041 tcgccgacgg ccaccgggtc gccaccgtct cccgctccgg cggggaggtc gacggcgcgc
     2101 tcgccgtcgc ctgcgacatc gccgatccgg ctgcggtcga cgccgccatc gccacggtcg
     2161 aggagcagct cgggcccatc gaggtcctgg tcgccaacgc cggcatgacc aaggacggct
     2221 tgctggtgcg gatgtccgac gacgacttca ccagcgtcat cgagaccaac ctcaccggca
     2281 gcttccgctt cgcccgagcc gtcaccaaga acatgatgcg ggcccggttc ggtcgcatcg
     2341 tcttcatgtc ctcggtcggc gcctacatgg gtgcggccgg gcaggcgaac tacgccgcgt
     2401 ccaaggccgg cgtcatcggc ctggctcggt ccatggcccg ggagctggcc agccgcagcg
     2461 tcacggtgaa cgtggtgacc cccggcccca tcgacaccga catgttcgcc gccgtcaccg
     2521 acaagcggcg cgacgagctg gccgccaccg tgcccatgaa ccgattgggt tcgcccgacg
     2581 aggtcgccgc cgtcgtacgg ttcctggcgt ccgacgaggc cagcttcgtc accggcgccg
     2641 tggtgccggt ggacggcggc ctcggcatgg gcttctagac cccacccctt tcgaacaatc
     2701 ccccttcaac acaggagaca agtgcagtga gcgacgactt cgagcggttc cagaagtgcg
     2761 cggtggaggt cctctccgtg gaggccaccc aggtcaccaa ggaggcgagc ttcgccgacg
     2821 acctcgacgc cgacagcctc gacctggtcg agctggtcat ggccctcgag gaggagttcg
     2881 acgtgaccgt cgaggaggag gagctggagg gcatcgagac cgtcggtgcc gccttcgacc
     2941 tcatcaaggg caagctctag caagggcagg acggcaccga tgaccgacga tcgcaaccaa
     3001 cgcacccgcc gccgggtcgc catcacggcg gtcagcgcgg tggccaaggc cggcatcggt
     3061 gccgagcagt tctgggacgg gctgtgcacc gcccccgagg acggcatg
//
LOCUS       DATISU010000424         8290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_828834, whole genome shotgun sequence.
ACCESSION   DATISU010000424 DATISU010000000
VERSION     DATISU010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828834"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1050)
                     /locus_tag="VLR27_16880"
     CDS             complement(136..1050)
                     /locus_tag="VLR27_16880"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HSP05182.1"
                     /translation="MDPDGTIRADGVEVSWEDARADVAYGSADIFDDQSLTRRFFGWL
                     LRAEQLQWLQMAAAGVDHPVFGALLDGGVRITSAHVTDIPIAEYVLAQVLRATVPIEA
                     MDASTAQHQWTKHEWREVHGSRWLVVGLGSIGSAVAVRAQAFGAHVTGIRRSPTGDEP
                     VDEMIAPDDLLAAVPNADVVVLCTPATRHTVGMVDAGFLGRMAPQSILVNVGRGQLVD
                     EVALRRALDGGRPARAILDVAVDEPPADDSWLWDHPSVTLTAHTSAGGDGRHARAADV
                     FAGNLARFVAGQPLADEVTADSREAPPG"
     gene            complement(1289..3397)
                     /locus_tag="VLR27_16885"
     CDS             complement(1289..3397)
                     /locus_tag="VLR27_16885"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HSP05183.1"
                     /translation="MEAIPYLALVSALAGLGLAFYYFGLVKKESSGNERMTELMLEIQ
                     AGAKAFLKQEYTYVAIFVVVLAILVAILVNPLGAITYVLGAALSALAGYVGMTVATMA
                     NARTTEAARVGGPAKALPLAFRGGAVMGFSVAGLALAGLAIVYIVFVQLLEVDEAFEI
                     LTAFGLGASSIALFSRVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPATIADNVGDN
                     VGDVAGMGADLFESYGGSIVAPIALIAFTVFAVSPDMSTAELLTSDPTLKLLLFPFGV
                     ALIGMVASILGSFTVKAGDSTDIHALSKALHMGTNVAMGITVVGVLALGMVMFSGDEF
                     DNAWGLPLSVIGGLLVGYAIGKVAEIWTSDQYGPVKKIAAASETGPATTILGGISAGM
                     ISVGASVVLIGAGVFVAYWGGEWAGDGLGIYGIAIAAIGMLATTGVVVSVDAYGPIAD
                     NAGGIAEMAELDPSVREVTDALDSLGNTTAAIAKGFAVGSAALTALALFKTFEVALVQ
                     AGGDDFTGLNLNVGDVDVFIGLFLGAMLPFVFAALTIDAVGRAANQMIEEVRRQFREI
                     PGLREGKPGVRPDSARCVAISTKASLKEMIIPGALAVVVPLVIGFIDVDTLGGFLAGA
                     LVTGFCLAIFMANSGGAWDNAKKYIEQGNHGGKGSDPHKAAVVGDTVGDPFKDTSGPA
                     MNILIKVMTIVSLVFAGAFV"
     gene            complement(3441..4115)
                     /locus_tag="VLR27_16890"
     CDS             complement(3441..4115)
                     /locus_tag="VLR27_16890"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HSP05184.1"
                     /translation="MDTFSKSERELLKAIYRLSQGVNQAEAHTGALADKLGLSPSTIT
                     TAIKRLADRDLVDHTPYRGVELTETGSRIAVASIRRHRIVERFLADMLGYAWNEADRL
                     AATFEHELPQEVEDRLFVALDRPDSCPHGFPIPAPEVADIPPLPPLYDLEPGDIATVA
                     VPGSTDPEVVAFLDQLGLRPGGRVQIQEKHPFDGPLVLTIDGQTRTLGATVANQVFVQ
                     REHAAT"
     gene            4265..5608
                     /gene="trkA"
                     /locus_tag="VLR27_16895"
     CDS             4265..5608
                     /gene="trkA"
                     /locus_tag="VLR27_16895"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HSP05185.1"
                     /translation="MYVIVVGAGEVGSYVADRLSREHHDVAVIDRDRVALRDVEEHLD
                     VLTVLGSGTNPEILRAAGIDKAEMVVAVTSQDEVNLVCCLVGKQAGVGRTVCRLEDPE
                     LRSRKSAALHDAMGVDLIIDPDFQTSQEILELLEYPGASEVAEMAGGEVVVIGARLPA
                     DAPVVGRSLVEIARDHEPEWEFLFGVITRGSETIIPRGDVTLEADDLVRVLCKRKARR
                     QLMRLLGLARKAPRRIMLLGGGRTAELVAVALESRGSEVVIVERDDDRANELAERLGG
                     ALILKGEITDADLLEESDVGAFDAVLALTGEDDANILACLYAKLQGAKETIAVVHRLS
                     LLPLLAEAGIDVALSPRTASANGVLRFVRGDISAVATFLQGEVEVLEVEVREGSEADG
                     GIIAELRLPKDVLVGAYVRDGKAQIARGRSELRDRDHVVLFAVPGSVDSVRRVFS"
     gene            5615..7180
                     /locus_tag="VLR27_16900"
     CDS             5615..7180
                     /locus_tag="VLR27_16900"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HSP05186.1"
                     /translation="MPTRPPRDALIEADPSLLRAIARAGHVESAVGFVVGVALLACGV
                     LALVSAAAGLIDGRDPEILALLAAVWAVAGGLLVRSCHPPARLRSSTAFAGVLASWLT
                     LIAVSTVTYEVLGTFEHLDDSLFESVAGFTTTASSVLGDPESVARGVLAWRAGTQWLG
                     GLAALVFVVAVLPSIGVGGLDVTSAGYRYSGTSLRSHRTLASMRRLLTLYVIFTVAGV
                     GLYLVAGMGPFDAVTYAATTISTGGFANHAGSFSHFESTAVEWAGFGGMVLGGANMAL
                     LYRTLRGRDLRGAVRSFELRAYLAAIVVGGTIVTLATAPTGGITHESLRHAYFHVASA
                     TSTTGHWVGLGWGEWSVAPQVLLLAIMGVGSMSGSAGGGFRQVRALALVGYLRRELIL
                     QLHPRAVAAVRVGRRPVSEELVGRMIGYQAQYLMVAGLGAVVVAVLGGDLVTSITGSI
                     SALANVGPALGELVPGAGGIRVLSRPERAALLPLMLLGRLEIAPVLVGLGLAATGVRR
                     VVQRGAGRGDTVD"
     gene            complement(7190..8161)
                     /locus_tag="VLR27_16905"
     CDS             complement(7190..8161)
                     /locus_tag="VLR27_16905"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP05187.1"
                     /translation="MAGVTEEPGADSAAPIATEPAPVLLADIADADRSGRNPLADDPV
                     EDDGTSKKRPLGITFWLAVAWIAVVGIAGLFSGFLPTADPNAIDIPAKLTQPFTDGYV
                     LGTDGLGRDILARLVDGARVSLTVSLTAVAVGMLVGGLLGTLAGFVRGFFDVFFVAGI
                     NIMLAFPGIVLLLALVSVLEQNLVNISLVIGVLSIPIYARVARANSLAVSERDYVLAA
                     RALGATQKRILFREILPVVLMPLLAFALTALGVVIVAEGTLAFLGLSVEPPAATWGSM
                     IAEGRRHDETHLHPVIIPSLALFLTVLSFNFVGEALRARFADLDAKV"
BASE COUNT         1160 a         2763 c         3174 g         1193 t
ORIGIN      
        1 tgccgctgaa ggcccgctcg gtgccggcgt tctgggtgac ctcgaactgc tccggggtca
       61 ggcgctcgcg cagctccgct tcgctcggtg gcgtgaagtc cgtgtcctgc tcggtcatgg
      121 ccccagtgtg ccgggtcagc ctggtggcgc ctcgcgcgag tcggcggtga cctcgtcggc
      181 gagaggctgc ccggcgacga accgggcaag gttgccggcg aagacgtcgg cggcgcgggc
      241 gtggcgcccg tcgccaccgg ccgaggtgtg ggcggtgagc gtgaccgacg ggtggtccca
      301 cagccagctg tcgtccgccg ggggctcgtc gacggcgacg tcgaggatgg cccgcgccgg
      361 ccggccaccg tcgagggcgc gccggagcgc gacctcgtcg acgagctggc cccgcccgac
      421 gttgacgagg atcgactgcg gcgccatgcg gccgaggaag ccggcgtcga ccatgccgac
      481 cgtgtggcgg gtggccggcg tgcagaggac gacgacgtcg gcgttcggga cggcggccag
      541 gaggtcgtcc ggtgcgatca tctcgtcgac cggctcgtcg ccggtggggg agcgacggat
      601 gccggtgacg tgggcaccga aggcctgcgc ccgcaccgcg acggcgctgc cgatggagcc
      661 gaggccgacc accagccacc ggctgccgtg gacctcgcgc cactcgtgct tggtccactg
      721 gtgctgcgcc gtgctggcgt ccatggcctc gatcggcacc gtggctcgca ggacctgggc
      781 gagcacgtac tcggcgatgg ggatgtcggt cacgtgcgcg ctggtgatgc gcacgccgcc
      841 gtcgagcagc gccccgaaga cggggtggtc cacgcctgcg gcggccatct gcagccactg
      901 gagctgctcg gcccggagca gccagccgaa gaaccggcgg gtcagggact ggtcatcgaa
      961 gatgtcggcc gagccgtagg cgacgtcggc ccgggcgtcc tcccaggaga cctcgacgcc
     1021 gtcggcccgg atggtgccgt ccgggtccat ggccaggaag cgcgccgcgg gtgcggcggc
     1081 ctggaggcgg tcgccgaacg tttccagctg gtgcgcggtg acgaggacgg tcgtgatcgg
     1141 ggcagcgtac gcggcccagc ttctgggccg cggtgcacgc ctccagggcg tgtgcgactg
     1201 cccggttcgc gcgagctggg cccggacgcg ccggtgccgg cgcccccgag ggagcgccgg
     1261 caccggttcg actgcgtcga gggtcagatc agacgaaggc gccggcgaac accagcgaga
     1321 cgatcgtcat caccttgatg aggatgttca tggccgggcc ggaggtgtcc ttgaacggat
     1381 cgcccaccgt gtcgcccacg acggcggcct tgtgcgggtc ggagcccttg ccgccgtggt
     1441 tgccctgctc gatgtacttc ttggcgttgt cccaggcgcc accggagttg gccatgaaga
     1501 tggccaggca gaagccggtg acgagggcgc cggcgaggaa gccgccgagg gtgtcgacgt
     1561 cgatgaagcc gatgaccagg ggcacgacga ccgcgagggc gccggggatg atcatctcct
     1621 tcagcgaggc cttggtggag atggccacgc agcgggccga gtccggacgc acgcccggct
     1681 tgccctcgcg caggcccggg atctcccgga actggcgacg cacctcttcg atcatctggt
     1741 tcgcagcccg accgacggcg tcgatcgtga gggcggcgaa gacgaagggc agcatggcgc
     1801 cgaggaacag gccgatgaac acgtcgacgt cgccgacgtt caggttcaga ccggtgaagt
     1861 cgtcgccacc ggcctggacc agtgcgacct cgaaggtctt gaacagggcc agtgcggtga
     1921 gggcagccga gccgacggcg aagcccttgg cgatggcggc cgtcgtgttg ccgaggctgt
     1981 cgagggcatc ggtgacctcg cggacgctcg ggtccagctc ggccatctcg gcgatgccgc
     2041 cggcgttgtc ggcgatgggg ccgtaggcgt cgacggagac cacgacgccg gtggtggcca
     2101 gcatgccgat ggcggcgatg gcgatgccgt agatgccgag gccgtcgccg gcccactcgc
     2161 cgccccagta ggccacgaag acaccagcgc cgatcaggac gacggaggcg ccgacggaga
     2221 tcatgccggc ggagatgccg ccgaggatgg tggtggccgg gccggtctcg gaggcggcag
     2281 cgatcttctt gaccgggccg tactggtccg aggtccagat ctcggcgacc ttgccgatgg
     2341 cgtagccgac caggagacca ccgatgaccg acaggggcag gccccaggcg ttgtcgaact
     2401 cgtcgccgct gaacatgacc atgccgaggg cgagcacgcc gacgacggtg atgcccatgg
     2461 ccacgttggt gcccatgtgg agcgccttcg acagggcgtg gatgtcggtg gagtcaccgg
     2521 ccttcaccgt gaaggaaccg aggatggagg cgaccatccc gatgagcgcc acgccgaagg
     2581 ggaacagcag gagcttgagg gtcgggtcgc tggtgagcag ctcggcggtg ctcatgtccg
     2641 gggacacggc gaacaccgtg aacgcgatca gggcgatcgg ggccacgatg gagccaccgt
     2701 aggactcgaa caggtcggcg cccatgccgg cgacgtcgcc gacgttgtcg cccacgttgt
     2761 cggcgatggt cgccgggttg cgggggtcgt cctcggggat gcccgcctcg accttgccga
     2821 cgaggtcggc gccgacgtcg gcggccttgg tgtagatgcc gccaccgacg cgggagaaca
     2881 gcgcgatgga gctggccccg agaccgaagg cggtgaggat ctcgaaggcc tcgtcgacct
     2941 cgagcagctg cacgaagacg atgtagacga tggccaggcc ggccagcgcg aggccggcga
     3001 ccgagaagcc catgacggcg ccgccgcgga aggcgagcgg cagggccttg gccggaccac
     3061 ccacgcgggc ggcctccgtc gtgcgggcgt tggccatggt ggccacggtc atgccgacgt
     3121 agccggccag ggccgacagc gccgcgccga gcacgtaggt gatggcgccg agcgggttca
     3181 cgaggatcgc gaccaggatg gcgaggacga ccacgaagat cgccacgtag gtgtactcct
     3241 gcttcaagaa ggccttggca ccggcctgga tctcgagcat cagctcggtc atccgctcgt
     3301 tgcctgagct ctccttcttg accaggccga agtagtagaa cgcgaggccg agaccggcca
     3361 gcgcactcac caaggcgagg taggggatgg cttccactgg gattgtctcc tgtcgtgggt
     3421 gtgtacgggt gtagggcggt tcaggtggcg gcgtgctcgc gctgcacgaa cacctggttg
     3481 gcgacggtgg caccgagggt gcgggtctgg ccgtcgatgg tcagcacgag cgggccatcg
     3541 aaggggtgct tctcctggat ctgcacccgt ccgcccgggc gcagccccag ctggtcgagg
     3601 aacgcgacga cctcggggtc ggtcgagccc ggcaccgcca cggtggcgat gtcgccgggc
     3661 tcgaggtcgt agagcggcgg cagcggcggg atgtcggcca cctcgggggc ggggatcggg
     3721 aagccgtgcg ggcacgagtc gggccggtcg agggccacga acagccggtc ctcgacctcc
     3781 tggggcagct cgtgctcgaa ggtcgcggcc aggcggtcgg cctcgttcca ggcgtagccg
     3841 agcatgtcgg ccaggaagcg ctcgacgatg cggtggcggc ggatgctggc cacggcgatg
     3901 cggctgccgg tctcggtgag ctccacgccc cggtagggcg tgtggtcgac caggtcccga
     3961 tcggccaggc gcttgatggc cgtggtgatc gtggacgggg acagcccgag cttgtcggcg
     4021 agcgcgccgg tgtgggcctc ggcctggttg acgccctggg agaggcggta gatggccttc
     4081 aggagctctc gctcggactt ggagaaggtg tccatcggac gtgggtctcc cgcgacgggg
     4141 ctcggcggcg gtgcggcacc ctcgggctgg cgggtgcaga acgggcggac cgtaagtttc
     4201 ggagcccgaa aaagtcaact tcgtgccggt cgtggggcag gatggcgccc gtggaggtgc
     4261 ggccgtgtac gtgatcgtcg tcggcgccgg cgaggtcggc agctacgtgg ccgatcggct
     4321 cagccgggag caccacgacg tcgccgtgat cgaccgggac cgcgtcgccc tgcgcgacgt
     4381 cgaggagcac ctcgacgtgc tcacggtgct cgggtccggc accaaccccg agatcctgcg
     4441 ggcggccggc atcgacaagg ccgagatggt cgtggccgtc accagccagg acgaggtcaa
     4501 cctggtgtgc tgcctcgtgg gcaagcaggc cggggtcggg cgcaccgtgt gccgcctcga
     4561 ggacccggag ctgcgctccc gcaagtccgc ggcgctgcac gacgccatgg gcgtggacct
     4621 catcatcgac cccgacttcc agacctccca ggagatcctg gagctgctcg agtacccggg
     4681 ggcgtcggag gtggccgaga tggccggtgg cgaggtcgtc gtcatcggtg cccgcctgcc
     4741 ggcggacgcc ccggtggtgg gacggtccct ggtcgagatc gcacgggacc acgaaccgga
     4801 gtgggagttc ctcttcgggg tcatcacccg cggttcggag accatcatcc cccgtggcga
     4861 cgtgaccctc gaggccgacg acctggtccg ggtgctgtgc aagcgcaagg cccgccgcca
     4921 gctgatgcgg ctcctcggtc tggcccgcaa ggcaccccgc cggatcatgc tcctcggcgg
     4981 tggtcgcacc gccgagctgg tggcggtcgc gctggagtcg cgggggagcg aggtcgtcat
     5041 cgtcgagcgc gacgacgacc gggccaacga gctcgccgag cggctgggcg gggcgctgat
     5101 cctgaagggc gagatcaccg acgccgacct gctcgaggag tccgacgtcg gggccttcga
     5161 cgccgtcctg gccctcaccg gcgaggacga cgccaacatc ctcgcctgcc tctacgccaa
     5221 gctccagggg gccaaggaga ccatcgccgt ggtccaccgc ctgtcgctgc tgcccctgct
     5281 ggccgaggcc ggcatcgacg tggccctctc accccgcacg gccagcgcca acggcgtgct
     5341 gcgcttcgtc cgcggcgaca tctccgcggt ggcgacgttc ctgcagggcg aggtcgaggt
     5401 gctcgaggtc gaggtacggg agggcagcga ggccgacggc ggcatcatcg ccgagctgcg
     5461 gctcccgaag gacgtgctcg tcggcgccta cgtgcgcgac ggcaaggccc agatcgcccg
     5521 ggggcgctcc gagctgcggg accgggacca cgtggtgctg ttcgcggtgc ccggctcggt
     5581 ggacagcgtg cggcgcgtgt tcagctgacg acccgtgccg acccgaccgc cgcgcgacgc
     5641 tctcatcgag gcggacccga gcctcctccg tgcgatcgcc cgggccgggc acgtcgaatc
     5701 ggccgtgggg ttcgtggtcg gcgtggccct cctcgcctgc ggcgtcctcg cgttggtcag
     5761 cgcggcggcg gggctcatcg acggccgtga cccggagatc ctggccctgc tggccgccgt
     5821 ctgggcggtc gccggcggcc tgctggtccg ttcctgccac ccgccggctc ggctccgctc
     5881 gagcacggcg ttcgcgggcg tgctggcctc gtggctgacg ctcatcgccg tgtcgacggt
     5941 gacctatgag gtcctcggca ccttcgagca cctcgacgac tcgctcttcg agtcggtcgc
     6001 cgggttcacc acgacggcgt cgtcggtgct cggcgatccc gagagcgtgg cccggggcgt
     6061 gctggcgtgg cgggccggga cccagtggct cggcgggctg gcagcgctcg tgttcgtcgt
     6121 cgccgtcctg ccctcgatcg gggtcggcgg cctcgacgtc acctcggccg gctaccggta
     6181 ctcgggaacg tcgctgcgca gccaccggac cctggcctcg atgcgccggc tcctcaccct
     6241 ctacgtcatc ttcacggtgg ccggcgtcgg cctctacctc gtcgccggca tgggcccctt
     6301 cgacgccgtc acctacgcgg cgaccacgat ctccaccggc gggttcgcca accacgccgg
     6361 ctccttctcc cacttcgagt cgaccgcggt cgagtgggcc gggttcggcg gcatggtgct
     6421 cggcggggcg aacatggccc tgctgtaccg gaccctgcgg ggacgggacc tgcgaggtgc
     6481 ggtgcgctcg ttcgagctgc gggcctacct ggccgccatc gtcgtcggcg gcaccatcgt
     6541 caccctggcc acggcgccga ccggcggcat cacccacgag tcgctgcgcc acgcctactt
     6601 ccacgtcgcg tcggcgacgt ccaccaccgg gcactgggtc ggcctgggct ggggcgagtg
     6661 gtcggtggcc ccccaggtgc tgctgctggc gatcatgggc gtcgggtcca tgtcgggctc
     6721 cgccggcggc gggttccgcc aggtgcgtgc gctcgcgctc gtcggctacc tgcgtcgcga
     6781 gctcatcctg cagctgcacc cccgggccgt cgccgcggtg cgggtcggcc ggcgtccggt
     6841 gagcgaggag ctggtcggcc ggatgatcgg ctaccaggcg cagtacctca tggtcgccgg
     6901 cctgggcgcg gtggtcgtgg cggtcctcgg cggcgacctc gtcacctcga tcaccgggtc
     6961 catctccgcg ctggccaacg tcggccccgc cctcggggag ctggtgcccg gcgccggcgg
     7021 catccgtgtc ctcagccgtc cggagcgggc ggccctgctg ccgctgatgc tcctcggacg
     7081 cctcgagatc gcccccgtgc tggtggggct gggcctggcc gccaccggag tccgccgcgt
     7141 cgtgcagcgc ggggcaggac gcggcgacac ggtggactga ggctcggcct cagaccttgg
     7201 cgtcgaggtc ggcgaagcgg gctcggagcg cctcgcccac gaagttgaac gacagcacgg
     7261 tgaggaacag cgccagcgac gggatgatca ccgggtggag gtgggtctcg tcgtggcggc
     7321 gaccctcggc gatcatcgag ccccaggtgg cggccggggg ctccacggag aggccgagga
     7381 aggccagcgt gccctcggcc acgatcacca cgccgagggc ggtgagggcg aaggcgagca
     7441 ggggcatcag caccaccggg aggatctccc ggaacaggat ccgcttctgg gtggcgccga
     7501 gggcgcgtgc cgccagcacg tagtcgcgct cggacacagc gagggagttg gcccgggcca
     7561 cccgggcgta gatggggatc gacaggacgc cgatgacgag ggagatgttc accaggttct
     7621 gctcgagcac cgagaccagg gccagcagca ggacgatgcc ggggaaggcg agcatgatgt
     7681 tgatcccggc gacgaagaag acgtcgaaga agccacgcac gaagccggcg agggtgccga
     7741 gcaggccgcc gaccaacatt cccacggcca cggcggtgag gctcaccgtg agcgacaccc
     7801 gagcgccgtc gaccagacgg gcgaggatgt cgcgcccgag cccgtcggtg ccgagcacgt
     7861 agccatcggt gaagggctgg gtgagcttgg cggggatgtc gatggcgttg gggtccgcgg
     7921 tggggaggaa ccccgagaac agcccggcga tgccgacgac ggcgatccac gccacggcca
     7981 gccagaacgt gatgccgagc ggccgcttct tggaggtgcc gtcgtcctcg accggatcgt
     8041 cggccagcgg gttgcgcccg ctgcggtcgg cgtcggcgat gtcggcgagc aggaccggtg
     8101 ccggctcggt ggcgatgggt gcggcgctgt ccgcgccggg ttcttcggtg acgccagcca
     8161 tggttcagac cttgatccgg gggtcgagca gccggtacac gatgtcgacc gcgaagttga
     8221 cgatgacgaa cgaggcggcg atcacgacga ccacgccctg gaccacgatg tagtcccgct
     8281 cgtagatggc
//
LOCUS       DATISU010000425         1909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_829227, whole genome shotgun sequence.
ACCESSION   DATISU010000425 DATISU010000000
VERSION     DATISU010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829227"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VLR27_16910"
     CDS             <1..623
                     /locus_tag="VLR27_16910"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HSP05188.1"
                     /translation="LGVTVNDVVLGMCAGALRTYLDKHGELPEESLRCLIPVSVRTDD
                     EALSYGNRVSGMLAALATDVADPIERLHVIHAETLRAKESLGALPADLLTDVAEFAPP
                     AIFARASRVMARTALANRIDPPFNLIISNVPGPQFPLYAGGAEMLSYIPVSAVADTQG
                     LNMTIMSYNGRCDFGLVACRDAVPDLWDLCHLLEDSLEELKDLAGV"
     gene            complement(635..>1909)
                     /locus_tag="VLR27_16915"
     CDS             complement(635..>1909)
                     /locus_tag="VLR27_16915"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HSP05189.1"
                     /translation="AGVITIEDGDFEASPFFPTGRITTRTEGRAEVPDDVAVLHGGIW
                     DVGKEWRTTSTTPDFPTAGAIFDQLAELAPIGPVDMVVSLDVPALAMMLDATGPVEVD
                     GEVVSSANAVDLLLRDNYLRMGEESEANERRDLQSRIAGTIFETVTERDLDIVDLAAS
                     LTRAAEGRNLMGWSEDPGVQALWESLGADGAVTEDSFLVAAQNASASKRDYYLDPEVD
                     VIPVGPPVDGQRRYRATMTLENPVVSPTAPYVDSLNRFVPVGVHRAYVTFTLPGAATD
                     VEVVQGTHSGVGPEGPTMVAAAWIRVPESEQRSAAVEFSLPVDQRTVELVPGARVRPT
                     TYRFGPHLELTDATARRVPLPRVATLRPQKAQPLAAGALVVAFAGILLGVSRSRRLAR
                     PEPDVVGARADGRFAIGTFALALALAVLAGVG"
BASE COUNT          254 a          696 c          699 g          260 t
ORIGIN      
        1 cgctcggcgt cacggtcaac gacgtcgtgc tcggcatgtg cgccggtgcg ctgcgcacct
       61 acctggacaa gcacggcgag ctgcccgagg agtcgctgcg ctgcctgatc ccggtgtcgg
      121 tccgcaccga cgacgaggcc ctcagctacg ggaaccgggt gtcgggcatg ctcgccgccc
      181 ttgccaccga cgtggccgac ccgatcgagc ggctccacgt catccacgcc gagacgctgc
      241 gggccaagga gagcctcggc gccctcccgg ccgacctgct caccgacgtc gccgagttcg
      301 ccccgccggc catcttcgcc cgcgcctcgc gggtcatggc ccgcacggcc ctcgccaacc
      361 ggatcgaccc gccgttcaac ctgatcatct ccaacgtgcc cggcccgcag ttcccgctct
      421 acgccggcgg cgccgagatg ctgtcgtaca tcccggtgtc cgccgtcgcc gacacccagg
      481 ggctgaacat gacgatcatg tcctacaacg gccggtgcga cttcggcctc gtcgcctgcc
      541 gcgacgccgt ccccgacctg tgggacctgt gccacctgct cgaggactcg ctcgaggagc
      601 tcaaggacct cgccggcgtg tagctgtcgc ccgctcagcc gacgccggcg agcacggcga
      661 gggcgagggc gagggcgaac gtcccgatgg cgaaccggcc gtcggcccgg gcgcccacga
      721 cgtcgggctc gggccgggcc agtcgccgcg accggctcac cccgagcagg atcccggcga
      781 aggccacgac cagcgcgccg gccgccaggg gctgggcctt ctggggtcgc aacgtggcga
      841 ccctcggcag gggcacccgc cgggcggtgg cgtcggtgag ctcgaggtgc ggtccgaacc
      901 ggtaggtggt ggggcggacg cgggcgccgg gcaccagctc gaccgtccgc tggtccacgg
      961 ggagggagaa ctcgacggcg gcgctgcgct gctcggactc gggcacccgg atccacgccg
     1021 cggcgaccat cgtcggcccc tcgggcccca cgccgctgtg cgtcccctgc acgacctcga
     1081 cgtcggtggc ggcgcccggg agcgtgaagg tcacgtaggc ccggtggacg ccgacgggca
     1141 cgaagcggtt gaggctgtcg acgtaggggg cggtggggga gacgaccggg ttctcgagcg
     1201 tcatggtcgc ccggtagcgg cgctggccgt cgacgggcgg gccgaccggg atcacgtcca
     1261 cctccgggtc gaggtagtag tcccgcttgg aggccgacgc gttctgggcg gcgacgagga
     1321 agctgtcctc ggtgacggcg ccgtcggccc cgagggactc ccacagcgcc tgcacgccgg
     1381 ggtcctccga ccagcccatc aggttgcgac cctcggcggc ccgggtgagc gatgcggcga
     1441 ggtcgacgat gtcgaggtcc cgctcggtga ccgtctcgaa gatggtgccg gcgatgcggc
     1501 tctgcaggtc gcggcgctcg ttcgcctcgg actcctcacc catgcgcagg tagttgtcgc
     1561 gcagcagcag gtcgacggcg ttggcgctgg acacgacctc accgtcgacc tcgaccggcc
     1621 cggtggcgtc gagcatcatc gccagggcgg ggacgtcgag ggacacgacc atgtcgaccg
     1681 ggccgatcgg ggccagctcg gcgagctggt cgaagatcgc cccggcggtg gggaagtcgg
     1741 gcgtggtgga cgtggtgcgc cactccttcc cgacgtccca gatgcccccg tgcagaacag
     1801 ccacgtcgtc ggggacctcg gcccgaccct cggtccgggt cgtgatccgg ccggtgggga
     1861 agaaggggct ggcctcgaag tccccgtcct cgatcgtgat caccccggc
//
LOCUS       DATISU010000426         8742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_457958, whole genome shotgun sequence.
ACCESSION   DATISU010000426 DATISU010000000
VERSION     DATISU010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8742
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457958"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1279
                     /locus_tag="VLR27_16920"
     CDS             86..1279
                     /locus_tag="VLR27_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05190.1"
                     /translation="MPVEDQFRSWSELNVQPFDKLEVDPYTRTRVILMNGIEVESIMF
                     SHQFARHTDNLEIKRALATSRRADAMQQKVVNGLNPGDQTPLETTIGYEQVAVDLTAW
                     LARHEPDPMLKQALDFALLEDFDHLYRYANLYELIDGKDATELTEHLTEITPGRPTVD
                     EHRHPADDVRGHYDTHTVDPLSRLHVMTIISAEQQTMNFYMNHGTDWIEPIARGLYAE
                     IALIEEQHVTHYESLLDPLDSWLKQLVFHEYNEVYLYWSMLQQERDRRIKAIWELHLD
                     MEIGQLHAACDLLRRFDGVEPEEILPAELPDTPVTFEPNKAYVRDVLAAQVDLRADGL
                     DYVPVQALPSDHRYYRYQDTVNAGGTPSEQVIDQNRTRSGREFRDETEGEHPIIELRD
                     TAADR"
     gene            1289..2392
                     /locus_tag="VLR27_16925"
     CDS             1289..2392
                     /locus_tag="VLR27_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05191.1"
                     /translation="MQTLTPTKDTGATAKANEVVYVELDQTETDQGLATQLGLDGVNA
                     AFVADFLSACLTHERCGTHLYRSVAARTLNPMLQGKYQQFGRQTVRHVEILEDVIVAG
                     GGNSSYVSPLARATEAADTKALEATYLGSGGLDPMAAEMAMLDAVFMAETVDHANWEA
                     LGQLAAALPDGAMKDALTAAVREVEEDEDEHLSWARDTRARLTMLQASSSTVTEAMAT
                     GEELVARIKNWFTGPDSTIGDADARPTVATASKRSATKKRTAAKKTATKKKAAAKKKA
                     AAKKSATRKKTATKKAAAPKKKAAAKRSTASKKKAASKKKAGAKKKTASKKSAAKKKA
                     ATKKRSAAKKRTPSKKAAAKKRSTAKKATKRSR"
     gene            2429..3196
                     /locus_tag="VLR27_16930"
     CDS             2429..3196
                     /locus_tag="VLR27_16930"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HSP05192.1"
                     /translation="MDDHGDVGADDHAALTYGSYLALDEVLGAQRPRSDEHDELLFIV
                     IHQVYELWFKQLLHELAHLQSLLERGDTPHALRTLRRILTILKVAVAQIDVLETMSPR
                     EFSSFRDRLDAASGFQSAQFRELEAVLGRRDPAVFRHYPEGSGSRASIEAAMARPSLF
                     DSYLTYLGEQGFTVGDDVEEVLLAAYADDGGPAQVAEHLVDLDEGLQEWRYRHVKMVE
                     RTIGTKTGTGGSAGVEYLRTTLSDPAFPALWAVRSRL"
     gene            3193..4407
                     /locus_tag="VLR27_16935"
     CDS             3193..4407
                     /locus_tag="VLR27_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSP05193.1"
                     /translation="MTVTLDDLRRSPNALAAHYSRFDVGGGRILLSGHSHQAWPDVAE
                     AGLLECFADAADAVDGKWALAEAKVEQVRDGYRRFLSDPGGEVALGLNTHDLIVKLLS
                     CLDLRRRPRLVTTEGEFHSLRRQLDRLAEEGLEVVRVPVEPVDTLAERLAGEVDERTA
                     LVGVSAVLFMTAQVVPGLAAVAEACERHGADLLVDAYHALGTVVFDVHELGLASAWVT
                     GGGYKYLQLGEGNAFLRIPPHRHDVRPAITGWFAEFDLLTTGRDDPHHVAYGTPASRF
                     ASGTYDPASAYRGARVFAFFDDQGLTPALLRESYQHQLGVLAGAFDALDLPPEVIDRD
                     RRTPIAGLGGFLSLVTPHAGEVRAALTGRGVLTDSRADRLRVGPAPYLSDRQLEAGMA
                     VIGEVAVAASRG"
     gene            complement(4404..4643)
                     /locus_tag="VLR27_16940"
     CDS             complement(4404..4643)
                     /locus_tag="VLR27_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05194.1"
                     /translation="MGAEEEETPMHPFQSEIHARTELDARWRDAAERRLVRQATPAWS
                     QRTGALLVAVGDRFARTGRRLQSARPAPAACPEVC"
     gene            4694..5863
                     /locus_tag="VLR27_16945"
     CDS             4694..5863
                     /locus_tag="VLR27_16945"
                     /inference="COORDINATES: protein motif:HMM:NF039924.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5937 family protein"
                     /protein_id="HSP05195.1"
                     /translation="MAVTLTFPSDRPVDLIGFAWSPLAEAVLSLRAVVQPKRTPQHLP
                     WVRRARELPDPLLAELRTLFRPFDTALPGLFEVGIHGDSPTFADELDAFLALDPIMVA
                     HELSLSCLGGCELMDESDAMGDAALEDGPFRRRVVEEAEARGHGALARTAYDDPVAIQ
                     RRYADALRAYWDGAFAAEWDRLLPRIEAEVTAGARALVTAGAPGLVRTLLPEGRWDPD
                     QRAIVIAKDWDGSCDIGERGGMVLVPTVYGWPNVLVGLAPPWPVSVYVPLRDLRTPEL
                     PSATDREIAAGFSALGDETRLQIAGLVADEPRSTKELAALLSLSDSSISRHLKILEAA
                     GLVRGARDGYFVLYELVPERIDVLGGALRGSLGLAPDVAGEVPALPVSVPRAPGG"
     gene            6042..6803
                     /locus_tag="VLR27_16950"
     CDS             6042..6803
                     /locus_tag="VLR27_16950"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP05196.1"
                     /translation="MPRKFVVAVGGMTAVALEALRTTWQVKRWFQEYLDQCQFLTSVC
                     ILPVMLVSIPFGATISLQLGQLVSQIGAQSFTGAAAVTATIQQVAPVATALLIAGAGG
                     SAMAADMGARNVRDELAAMEVMSVNPTHRLVTPRLYAAMTVGVLLVAVVIAAGTAGSF
                     FFNVLIQGVSPGAFFDGATSIVRLPDLAIALIKAGIFGFVAAVVACFRGMNCDRSPVG
                     VGKGVTEAVVQTFLAVFILNYVLTTIYLTLYPPTI"
     gene            6803..7678
                     /locus_tag="VLR27_16955"
     CDS             6803..7678
                     /locus_tag="VLR27_16955"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP05197.1"
                     /translation="MASITTTPRGQRMVRRVQRATARPVGAFAQLGSFLQFSGKALRA
                     IVTHIVLKRRFLPEVGRQISDIVVGTGAVVVGGGMVFVIGAMSMATGATVGIQGYEGL
                     RSLGAESLTGLVTSFGNVREVTPIIAGIALAAQVGSSFTAELGAKRISEEIDALEVMS
                     IPPIAYLVSTRILGALIAILPLYLISLFASFFAAEFVTTQLLGLSTGVYDYYFRLFLP
                     PIDVVYSLIKALVFAVLVVLIHCWYGFHATGGPEGVGVAVGKAIRTSIVVIVIVNLFL
                     SYVFWGTSTTVTLTG"
     gene            7675..8715
                     /locus_tag="VLR27_16960"
     CDS             7675..8715
                     /locus_tag="VLR27_16960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HSP05198.1"
                     /translation="MSARTPLTPTRQALVGLIGLVLLLGLAGVMLRAAGGAYADDYEL
                     TALVTRPGFNLDETSTVKVRGVTVGGVKGIDLLDDGTVELTLHMRKEVRIPESATARI
                     EPLSVFGPKFVDIIPGEGEGSGPFLDDGDQLAETVSPSEVVETLETFSDVLASFDPEK
                     LGTILTEMARGLDGLGEDLGATITASDQLAEDFIANRGEIDALLANSVVLGETLQDRG
                     DQLVRITETGASVLDLLRENEDELGSLLLSASQLSADLTTTVDAAAPDVGPLVTGLDR
                     VTAVLISQLGQLPTFTASNERIARFLGEDLIQWDIGDGRLGGVTRAIVVLDPCVIVPT
                     TQGCPPPEQVSP"
BASE COUNT         1290 a         3221 c         2981 g         1250 t
ORIGIN      
        1 gaggtctgtg acgaccgtct cagggacagg gagtggtccc gcgcgtcgat cggagaccct
       61 catgaacccg ttggatcagc caggcatgcc cgtggaggac cagttccgga gctggtcgga
      121 gctgaacgtc cagcccttcg acaagctcga ggtcgacccc tacacccgca cgcgggtgat
      181 cctgatgaac gggatcgagg tcgagtcgat catgttctcg caccagttcg cccgccacac
      241 cgacaacctc gagatcaagc gggcgctggc caccagccgt cgtgccgacg ccatgcagca
      301 gaaggtggtc aacggcctca accccggcga ccagacgccg ctcgagacga cgatcggcta
      361 cgagcaggtg gcggtggacc tcaccgcgtg gctggcccgc cacgagcccg acccgatgct
      421 caagcaggcg ctggacttcg ccctgctcga ggacttcgac cacctgtacc gctacgccaa
      481 cctctacgag ctgatcgacg gcaaggacgc aaccgagctc accgagcacc tcacggagat
      541 caccccgggt cggccgaccg tcgacgagca ccggcacccg gccgacgatg tccgcggcca
      601 ctacgacacc cacacggtgg acccgctgtc ccggctccac gtcatgacga tcatctcggc
      661 cgagcagcag acgatgaact tctacatgaa ccacgggacc gactggatcg agccgatcgc
      721 ccgtggcctc tacgccgaga tcgcgctgat cgaggagcag cacgtcaccc actacgagtc
      781 gctgctcgac ccgctcgaca gctggctcaa gcagctcgtc ttccacgagt acaacgaggt
      841 ctacctgtac tggtcgatgc tgcagcagga gcgggaccgg cgcatcaagg cgatctggga
      901 gctgcacctc gacatggaga tcggccagct ccacgcggcc tgcgacctgc tgcgccgctt
      961 cgacggcgtg gagcccgagg agatcctgcc cgccgagctg cccgacaccc ccgtgacctt
     1021 cgagccgaac aaggcctacg tccgagacgt cctcgccgcc caggtcgacc tgcgcgccga
     1081 cgggctcgac tacgtcccgg tgcaggcgct gccctctgac caccgctact accgctacca
     1141 ggacaccgtg aacgccggtg ggacgccgag cgagcaggtc atcgaccaga accggaccag
     1201 gtccggccgg gagttccggg acgagaccga gggcgagcac ccgatcatcg agctgcgcga
     1261 caccgcggcc gaccgctgag aggacgacat gcagacactc accccgacca aggacacggg
     1321 cgcgacggcc aaggccaacg aggtcgtgta cgtggagctc gaccagaccg agaccgacca
     1381 gggcctggcc acccagctcg gcctcgacgg cgtcaacgca gcgttcgtcg ccgacttcct
     1441 gagcgcctgc ctcacccacg agcggtgcgg cacccacctg taccggtcgg tggcggcccg
     1501 gacgctgaac ccgatgctgc agggcaagta ccagcagttc ggacgccaga ccgtccgcca
     1561 cgtcgagatc ctcgaggacg tgatcgtcgc cggcggcggc aactcgtcct acgtctcgcc
     1621 cctggcgcgc gccaccgagg cggccgacac caaggccctc gaggcgacct acctcggctc
     1681 gggcggcctc gacccgatgg ccgccgagat ggccatgctc gacgcggtgt tcatggccga
     1741 gaccgtggac cacgccaact gggaggccct cggccagctc gcggccgcgc tgcccgacgg
     1801 cgcgatgaag gacgccctga ccgcggccgt ccgagaggtc gaggaggacg aggacgagca
     1861 cctgtcctgg gcgcgggaca cccgggcccg gctgacgatg ctgcaggcca gcagctccac
     1921 ggtgaccgag gcgatggcga ccggtgagga gctggtcgcc cggatcaaga actggttcac
     1981 cggccccgac agcaccatcg gcgacgccga cgcccggccg accgtggcca ccgcgtcgaa
     2041 gaggtcggcg acgaagaagc ggaccgcagc gaagaagacg gccaccaaga agaaggctgc
     2101 cgcgaagaag aaggccgcag cgaagaagtc cgcgaccagg aagaagaccg cgaccaagaa
     2161 ggcggcggcc ccgaagaaga aggcagcggc caagcggtcg acagcgtcga agaagaaggc
     2221 cgcctcgaag aagaaggccg gtgcgaagaa gaagaccgcg tcgaagaagt ccgcagccaa
     2281 gaagaaggcc gccacgaaga agcggtccgc cgccaagaag cggaccccgt cgaagaaggc
     2341 ggcggccaag aagcgctcga cggcgaagaa ggccaccaag cggtctcgct gacctcggcg
     2401 gtgccgggcc ggtggtagga acggcggcgt ggacgatcac ggcgacgtcg gtgccgacga
     2461 ccacgcggcg ctgacctacg gcagctacct ggccctcgac gaggtcctcg gcgcgcagcg
     2521 gccccgctcc gacgagcacg acgagctgct cttcatcgtc atccaccagg tctacgagct
     2581 gtggttcaag cagctcctgc acgagctcgc ccacctgcag tccctgctgg agcgcggcga
     2641 cacgccccac gccctgcgca cgctgcgacg gatcctcacg atcctcaagg tggcggtcgc
     2701 ccagatcgac gtgctcgaga ccatgtcgcc gcgggagttc tccagcttcc gtgaccggct
     2761 cgacgcggcc agcgggttcc agtccgcaca gttccgcgag ctcgaggccg tgctcggccg
     2821 gcgcgacccc gccgtgttcc ggcactaccc cgaaggcagt ggttcgcggg cgtcgatcga
     2881 ggcggcgatg gcccggccgt cgctgttcga ctcctacctc acctacctcg gcgagcaggg
     2941 cttcaccgtg ggcgacgacg tcgaggaggt gctgctcgcc gcctacgccg acgacggcgg
     3001 cccggcccag gtcgccgagc acctggtcga cctcgacgag ggcctgcagg agtggcggta
     3061 ccgccacgtg aagatggtcg agcggaccat cggcaccaag accggcaccg gcgggtccgc
     3121 cggggtggag tacctgcgca cgacgctctc cgacccggcg ttcccagcgc tgtgggccgt
     3181 gcggagccgg ctgtgacggt gacgctcgac gacctgcgcc ggtcgcccaa cgccctcgcc
     3241 gcgcactact cgcgcttcga cgtcggcggt ggtcgcatcc tgctgtcggg ccactcccac
     3301 caggcctggc ccgacgtggc cgaggccggg ctgctcgagt gcttcgccga cgcggccgac
     3361 gcggtcgacg gcaagtgggc gctcgccgag gcgaaggtgg agcaggtgcg cgacggctac
     3421 cgccgcttcc tctccgaccc cggcggcgag gtcgccctcg gcctcaacac ccacgacctc
     3481 atcgtgaagc tgctctcctg cctcgacctg cgccggcgcc cccgcctggt caccaccgag
     3541 ggcgagttcc actccctgcg ccgccagctc gaccggttgg ccgaggaggg cctcgaggtc
     3601 gtgcgggtcc cggtcgagcc ggtggacacc ctggccgagc ggctggccgg cgaggtcgac
     3661 gagcgcaccg cgctggtggg ggtgtcggcc gtgctgttca tgaccgccca ggtcgtgccg
     3721 ggactcgccg ccgtggccga ggcgtgcgag cgccacggtg ccgatctgct cgtcgacgcc
     3781 taccacgccc tcggcaccgt ggtcttcgac gtgcacgagc tcgggctcgc atccgcatgg
     3841 gtcaccggcg gcggctacaa gtacctgcag ctgggggagg gcaacgcctt cctgcgcatc
     3901 ccgccccacc gtcacgacgt ccgcccggcg atcaccgggt ggttcgccga gttcgacctg
     3961 ctcacgaccg ggcgggacga tccgcaccac gtcgcctacg gcacgccggc gagccgcttc
     4021 gccagcggca cctacgaccc ggccagcgcc taccgcggcg cccgggtctt cgccttcttc
     4081 gacgaccagg gcctcacgcc ggccctgctg cgcgagtcgt accagcacca gctgggcgtg
     4141 ctcgcgggag ccttcgacgc cctggacctg ccccccgagg tcatcgaccg ggatcgacgc
     4201 acgccgatcg ccggcctcgg cggcttcctc tcgctcgtga ccccccacgc cggcgaggtg
     4261 cgggcggcgc tcacgggacg gggcgtgctc accgacagcc gggccgaccg gctgcgggtc
     4321 gggccggcgc cgtacctctc cgaccgccag ctcgaggccg gcatggcggt catcggcgag
     4381 gtcgccgtcg cggccagccg gggctagcag acctcggggc aggcggccgg ggccgggcgg
     4441 gccgactgga gccggcggcc ggtgcgggcg aagcggtcgc cgacggcgac gagcagcgcg
     4501 ccggtgcgct gggaccaggc gggcgtggcc tggcggacca ggcggcgctc ggcggcgtcg
     4561 cgccaccgag cgtcgagctc ggtgcgggcg tggatctcgg actggaacgg gtgcatcggg
     4621 gtctcctcct cttctgcccc caccatcaca tggtacgatc atctgatcaa ctgattccag
     4681 gaaaagcgga tcaatggctg tcaccctcac cttcccgtcc gatcgtcccg tcgacctgat
     4741 cggcttcgcc tggtcgcccc tggccgaggc cgtcctgtcg ctgcgcgccg tcgtgcagcc
     4801 caagcgcacg cctcagcacc tgccgtgggt gcgccgggcg cgggagctgc ccgatcccct
     4861 cctcgccgag ctgcgcacgc tgttccgccc cttcgacacg gccctgcccg ggctgttcga
     4921 ggtcggcatc cacggcgaca gccccacctt cgccgacgag ctcgacgcgt tcctcgccct
     4981 ggacccgatc atggtcgccc acgagctgag cctgagctgc ctcggcggct gcgagctcat
     5041 ggacgagtcc gatgcgatgg gcgatgccgc cctcgaggac gggcccttcc gccggcgggt
     5101 ggtcgaggag gcggaggccc ggggccacgg tgccctcgct cgaacggcct acgacgatcc
     5161 cgtggcgatc cagcgccgct acgccgatgc gctccgcgcc tactgggacg gggccttcgc
     5221 tgcggagtgg gatcgcctgc tgccccgcat cgaggccgag gtcaccgctg gggcgcgggc
     5281 gctcgtcacc gccggtgccc cgggcctggt ccgcaccctc ctgcccgagg gccgctggga
     5341 cccggaccag cgagcgatcg tcatcgccaa ggactgggac ggcagctgcg acatcggcga
     5401 gcggggcggc atggtcctcg tgcccacggt gtacgggtgg cccaacgtcc tcgtcggcct
     5461 cgcaccgccg tggccggtct cggtgtacgt gccgctccgc gacctgcgga ccccggagct
     5521 gccctcggcc accgatcggg agatcgccgc cggcttcagc gccctgggtg acgagacccg
     5581 gctgcagatc gccggcctgg ttgccgacga gccccgcagc accaaggagc tggccgcgct
     5641 gctcagcctg tcggactcct cgatctcccg gcacctgaag atcctcgagg cggccggtct
     5701 cgtgcgcggc gcccgggacg gctacttcgt gctctacgag ctggtccccg agcggatcga
     5761 cgtgctgggc ggcgccctgc gcggcagcct cgggctggcc ccggacgtcg ccggcgaggt
     5821 gccggcgctg ccggtcagcg tgccccgcgc cccgggcggc tgagcgcccg aggaaccgtc
     5881 gcgttcgggc gaccccgctg tgcgcaacgt gggctacgtt ctgtcgtcgg ggacgccgtc
     5941 gtggcgaggt gacgacggct gggcagcccc ggcgtccgcg ccggggtccg acacgttggc
     6001 tcggggggtc gagattggac ggtgctgggg cgctcctggc gatgccgagg aagttcgtcg
     6061 tcgcggtcgg tggcatgacc gcggtcgcgc tcgaggccct ccgcaccacc tggcaggtca
     6121 agcgctggtt ccaggagtac ctcgaccagt gccagttcct gacctcggtc tgcatcctgc
     6181 cggtgatgct ggtgtcgatc ccgttcgggg ccaccatctc cttgcagctc ggccagctcg
     6241 tctcccagat cggggcgcag tccttcaccg gtgcggcggc cgtgacggcc acgatccagc
     6301 aggtcgcccc ggtggccacg gcgctgctca tcgccggcgc cggcggctcc gccatggcgg
     6361 ccgacatggg ggcgcgcaac gtgcgcgacg agctcgccgc catggaggtc atgtcggtca
     6421 acccgacgca ccggctcgtg acgccgcgcc tctacgcggc gatgaccgtc ggcgtgctgc
     6481 tggtggccgt ggtgatcgcc gcggggacgg cgggctcgtt cttcttcaac gtcttgatcc
     6541 agggcgtgtc cccgggggcg ttcttcgacg gcgcgacgtc catcgtgcgc ctgcccgacc
     6601 tcgccatcgc gctcatcaag gccgggatct tcggcttcgt cgccgccgtg gtcgcctgct
     6661 tccgcggcat gaactgcgac cgcagcccgg tgggcgtggg caagggcgtc accgaggcgg
     6721 tcgtgcagac cttcctcgcc gtgttcatcc tcaactacgt cctgaccacc atctacctga
     6781 cgctctaccc cccgacgatc tgatggccag catcaccacc acgcctcggg gccagcggat
     6841 ggtgcgccgg gtgcagcgcg ccaccgcccg tccggtcggc gcgttcgccc aactcggctc
     6901 gttcctccag ttctcgggga aggcgctgcg cgccatcgtc acccacatcg tcctgaagcg
     6961 ccggttcctg cccgaggtcg gccggcagat ctccgacatc gtggtcggca ccggcgcggt
     7021 ggtcgtcggt ggcggcatgg tcttcgtcat cggcgccatg tccatggcca ccggggccac
     7081 ggtcggcatc cagggctacg agggcctgcg ctcgctcggg gccgagtcgc tcaccggcct
     7141 cgtcaccagc ttcggcaacg tgcgcgaggt cacgccgatc atcgccggca tcgccctggc
     7201 cgcccaggtc ggatcgagct tcacggcgga gctgggcgcc aagcgcatct cggaggagat
     7261 cgacgccctc gaggtcatga gcatcccgcc gatcgcctac ctggtgtcga cccggatcct
     7321 cggcgccctc atcgccatcc tgccgctgta cctgatctcg ctgttcgcca gcttcttcgc
     7381 cgcggagttc gtcaccaccc agctgctcgg gctctcgacc ggcgtctacg actactactt
     7441 ccggctcttc ctgcccccga tcgacgtcgt ctacagcctc atcaaggcgc tcgtcttcgc
     7501 cgtgctcgtg gtgctcatcc actgctggta cggcttccac gccaccggcg gtccggaggg
     7561 cgtcggcgtg gccgtcggca aggcgatccg cacgtcgatc gtggtcatcg tcatcgtcaa
     7621 cctgttcctg tcctacgtgt tctggggcac ctcgaccacg gtgaccctca ccggatgagc
     7681 gcccgcaccc cgctcacgcc gacccggcag gcgctggtcg ggctcatcgg gctcgtgctc
     7741 ctgctcggcc tggccggggt gatgctgcgc gccgcgggcg gggcctacgc agacgactac
     7801 gagctgaccg ccctcgtcac ccgtccgggc ttcaacctcg acgagaccag cacggtgaag
     7861 gtgcgcgggg tgacggtggg cggcgtcaag ggcatcgacc tcctcgacga cggcaccgtc
     7921 gagctgaccc tgcacatgcg caaggaggtc cggatcccgg agtcggccac ggctcggatc
     7981 gagccgctct cggtgttcgg gccgaagttc gtcgacatca tcccgggcga gggcgagggg
     8041 tccggaccgt tcctcgacga cggcgaccag ctggccgaga ccgtgtcgcc gtcggaggtc
     8101 gtcgagacgc tcgagacctt ctcggacgtc ctggcgtcct tcgaccccga gaagctgggg
     8161 acgatcctga ccgagatggc ccgggggctc gacggtctgg gcgaggacct gggcgccacc
     8221 atcacggcgt cggaccagct ggccgaggac ttcatcgcca accggggcga gatcgatgcg
     8281 ctgctggcga actccgtcgt cctgggcgag acgctccagg accggggcga ccagctcgtg
     8341 cgcatcaccg agaccggcgc ctcggtgctc gacctgctgc gggagaacga ggacgagctg
     8401 ggttcgctcc tgctgtcggc ctcccagctc tcggccgacc tcaccaccac cgtcgacgcc
     8461 gccgcgccgg acgtcgggcc gctcgtcacc ggcctcgacc gggtgaccgc cgtgctgatc
     8521 tcccagctcg gacagctgcc gacgttcacc gcctcgaacg agcgcatcgc ccgcttcctc
     8581 ggtgaggacc tgatccagtg ggacatcggc gacggtcgcc tcggcggtgt cacgcgagcc
     8641 atcgtcgtgc tcgacccgtg cgtgatcgtg cccaccacgc aagggtgtcc accacccgag
     8701 caggtgtcgc catgagcctg cgtcgccact ggcgcttcgc cg
//
LOCUS       DATISU010000427         7355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_551098, whole genome shotgun sequence.
ACCESSION   DATISU010000427 DATISU010000000
VERSION     DATISU010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551098"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VLR27_16965"
     CDS             complement(<1..446)
                     /locus_tag="VLR27_16965"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HSP05199.1"
                     /translation="MTGEHMSPDEFRRLGHEYVDWVASYWERVGELPVQSTVAPGEVF
                     ASLPAGPPATGADDLAAVLADVDELVLPGITHWQHPSFFAFFPANTSGPGVLADLLSS
                     GLGVNGMNWATSPACTEVEMRMLDWFVELLGLPDHLRHDHDGPGGGV"
     gene            484..2016
                     /gene="guaB"
                     /locus_tag="VLR27_16970"
     CDS             484..2016
                     /gene="guaB"
                     /locus_tag="VLR27_16970"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HSP05200.1"
                     /translation="MGARFDQSDAVHPRFAKLGLTYDDVLLVPGESDVLPHEVDTSSM
                     LTPGIRLALPIVSAAMDTVTEARMAIALARLGGIGIVHRNLSIEDQAVEVDRVKRSQS
                     GMIEDPVTLGADAHVYDALDLMAKYKVSGVPITDRDGTLVGLLTNRDLRFVDDHDQSI
                     SAVMRKPPLVTAPVGTTLDEARRILWQNRIEKLPIVDDAGKLCGLITIKDITKRDDFP
                     HATQDDRGRLRVGAAVGVGADALDRCEALVAAGVDVLIVDTAHGHSRGVLDTVKEVKS
                     RWQVEVVAGNIATADAVRALVANGADAVKVGIGPGSICTTRVVAGVGVPQITAVADCA
                     DAANELGVPVIADGGVQHTGDIAKALAAGASTVMLGSMLAGTDEAPGEVIVHQGERFK
                     QYRGMGSMGAMGDRSSGGDEQAEARSFSKDRYFQGHVDEVAKLVPEGIEGRVAYKGPV
                     APLIHQFAGGLRAAMGYCGTPTIDELRTESQFVRITSAALRESHPHDITITKEAPNYR
                     VK"
     gene            complement(2099..2668)
                     /locus_tag="VLR27_16975"
     CDS             complement(2099..2668)
                     /locus_tag="VLR27_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05201.1"
                     /translation="MGEDEASVPGGSARRILLFGVTGSGKTTLARSVGARTGIPWHSA
                     DDELGWLPGWVERPTDEQRKIAERITSGDAWILDTAYSRWRDLVMARVELVVALDYPR
                     WRSLSRLLRRTARRVVRRELVCNGNRETLRRALSRDSIIAWHFRTFREKRAHIDRWMA
                     DPAGPRIVRLRSPAETDRWLETLGPAPDS"
     gene            2723..3484
                     /locus_tag="VLR27_16980"
     CDS             2723..3484
                     /locus_tag="VLR27_16980"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSP05202.1"
                     /translation="MPAISKAAPVFSLDGRRALVTGASTGIGRAMAGALADAGAEVVL
                     VARTRADLGRAADEIGERATWIAADVGQEAEIHRICEHVGDVDILVNAAGVNLRPPMH
                     ELTTDDWAITMAVNLTAPFLLGQCLAPGMVERGWGRILHVGSQQSWRAFGNSGGYGAS
                     KAGLLGLTRSQAEAWSPAGVTVNCVIPGFVRTAMTERIFVEEPERAEALAARTMVGRN
                     GTPEDFAGIAVFLCSDAAAFVTGQAIAVDGGFSAT"
     gene            3501..4310
                     /locus_tag="VLR27_16985"
     CDS             3501..4310
                     /locus_tag="VLR27_16985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP05203.1"
                     /translation="MAASLRPVLDVLRCPDCGAALEPSGPDAVTCRTGHRFDLIAHRA
                     PDLARTVVQRDTPGQRAMRFRPLVSIYESIWRPLFTRLAGGTDPAAETDQLIEWLEPT
                     PGATVLDLACGPGNTTRQLAAGIPGASVVGLDLSVPMLAEAVRATPDSSPIGFARVDA
                     HTLPVGDATVDVVHCAAALYLFGDPAAVIAEVARVIRPGGRFIGMTLVAPLQPLGGVG
                     RRAEQTFARLSGLRYFGARELEEMCRAAGLEGFSARSQGGALLFVADRPGG"
     gene            complement(4324..4605)
                     /locus_tag="VLR27_16990"
     CDS             complement(4324..4605)
                     /locus_tag="VLR27_16990"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSP05204.1"
                     /translation="MIVAFVLIQARPDAITDLGHALADTDGVRETHSVAGSGTDLVAV
                     LAVPDHEAIATVVTEHISKLDGVIDTTTMIAFRQYSTAELDASYDDFVG"
     gene            4739..5164
                     /locus_tag="VLR27_16995"
     CDS             4739..5164
                     /locus_tag="VLR27_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05205.1"
                     /translation="MRRYLVVAHKTLGGAHLVEHLRELREEHPGCRFHLVVPMHHPDH
                     AWTEGEVRAGANRTLDEMLERLASMGMGATGEVGDANPVYAVGVVLRREGPDAFDGIV
                     LSTLPHGISRWWRFDVPSRMTAAYPGLPLTHLVSDEALV"
     gene            complement(5180..5851)
                     /locus_tag="VLR27_17000"
     CDS             complement(5180..5851)
                     /locus_tag="VLR27_17000"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HSP05206.1"
                     /translation="MSRGGPQRIPRPPGTRLGGPPSWASLPEELRHPSLDQVRAALAE
                     GPEPRRSPVEGLTSRCSAVLAPLYEHDGETHVVLTRRAWHMRSHTGEVSFPGGKQEPG
                     ETPWQTALREADEEVALDPASVEQIGELHHLATVTSRSSIVPYVGVLPERPRLVPNPN
                     EVDAILHVPLAELLDPEAYRQERWGLPGIDRPIHFFEIVGDTIWGATASMLVDLLTRI
                     TAPAG"
     gene            complement(5883..6065)
                     /locus_tag="VLR27_17005"
     CDS             complement(5883..6065)
                     /locus_tag="VLR27_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05207.1"
                     /translation="MEIPPPNPEKLLDHWMEWEKGEIPPGELMKQLKMHGMRQLLEGI
                     VAGSDGDGETVGDVLG"
     gene            complement(6161..>7355)
                     /gene="groL"
                     /locus_tag="VLR27_17010"
     CDS             complement(6161..>7355)
                     /gene="groL"
                     /locus_tag="VLR27_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223986.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HSP05208.1"
                     /translation="DPTIGEILADAIDKVGKDGVVTVEESNTFGMDLEFTEGMQFDKG
                     YLSPYFVSDAERQEAVLEDPYILLYGSKISSVQDVLPVLEKVMQSGKPLVIIAEDVEG
                     EALAVLVVNKIRGTFTAAAVKAPGFGERRKAMLQDMAILTGGQVISEEVGLKLESTTL
                     DLLGTARKVIITKDDTTIVEGGGSEADVAGRIEQIKREIDDTDSDWDREKLQERLAKL
                     SGGVAVVRVGAATEVELKEKKHRIEDALSATRAAIEEGVVAGGGTALLRARAAVQKVI
                     DSLEDDQQTGARAVYTALAAPARQIAVNAGMEGAVVVEAIERETGGNGFNAATGEYVD
                     LVKAGVIDPAKVTRAALQNAASIAAMVLTTEALVADKPEEEGAPMGDPTGGMGGMGGM
                     GGMGF"
BASE COUNT         1005 a         2695 c         2605 g         1050 t
ORIGIN      
        1 acgccgccgc cgggtccgtc gtggtcgtgg cgcaggtggt cgggcagccc gagcagctcg
       61 acgaaccagt cgagcatccg catctcgacc tcggtgcacg ccgggctcgt cgcccagttc
      121 atgccgttca cgccgaggcc cgaggacagc aggtcggcga gcacccccgg tcccgacgtg
      181 ttcgccggga agaaggcgaa gaacgacggg tgctgccagt gggtgatgcc gggcaggacg
      241 agctcgtcca cgtcggcgag caccgcggcg aggtcgtcgg cgcccgtcgc gggcgggccg
      301 gccggcagcg aggcgaagac ctcaccgggg gcgaccgtgg actgcaccgg cagctccccg
      361 acccgctccc agtacgacgc gacccagtcc acgtactcgt gtccgaggcg gcggaactcg
      421 tcggggctca tgtgctcgcc ggtcatctgc cgacgctagc cacgaccgcg ttaccctctc
      481 tccatgggag cgcgattcga ccagtccgac gccgtccatc cccggttcgc caagctgggc
      541 ctgacctacg acgacgtcct gctcgtcccc ggtgagtccg acgtcctccc ccacgaggtc
      601 gacacgtcgt cgatgctgac gcccggcatc cgcctcgcgc tgcccatcgt gtcggcggcg
      661 atggacaccg tcaccgaggc ccgcatggcc atcgcgctcg cccggctggg cggcatcggc
      721 atcgtccacc gcaacctgtc catcgaggac caggcggtcg aggtcgaccg ggtgaagcgc
      781 tcgcagtccg ggatgatcga ggaccccgtc accctcggcg ccgacgccca cgtgtacgac
      841 gccctcgacc tgatggccaa gtacaaggtc tccggcgtgc ccatcaccga ccgcgacggc
      901 acgctcgtcg gcctgctcac caaccgggac ctgcgcttcg tcgacgacca cgaccagtcg
      961 atcagcgccg tgatgcgcaa gcccccgctg gtcaccgccc cggtcggcac caccctcgac
     1021 gaggcccgcc ggatcctgtg gcagaaccgg atcgagaagc tgcccatcgt cgacgacgcc
     1081 ggcaagctct gcggcctcat cacgatcaag gacatcacca agcgagacga cttcccccac
     1141 gccacccagg acgaccgggg ccgcctgcgg gtcggcgccg ccgtcggcgt cggggccgat
     1201 gccctcgacc gctgcgaggc cctggtggcc gccggtgtcg acgtcctcat cgtcgacacc
     1261 gcccacggcc actcccgagg cgtgctcgac acggtgaagg aggtcaagtc ccgctggcag
     1321 gtcgaggtcg tggccggcaa catcgccacc gccgacgccg tacgggcgct ggtcgccaac
     1381 ggcgccgacg ccgtcaaggt cgggatcggg ccggggtcca tctgcaccac ccgggtcgtc
     1441 gccggcgtgg gcgtccccca gatcaccgcc gtcgccgact gcgccgacgc ggcgaacgag
     1501 ctcggcgtcc cggtcatcgc cgacggcggc gtccagcaca ccggcgacat cgccaaggcc
     1561 ctcgccgccg gcgccagcac cgtcatgctc ggctccatgc tcgccggcac cgacgaggcc
     1621 ccgggcgagg tcatcgtcca ccagggcgag cgcttcaagc agtaccgggg catgggctcg
     1681 atgggcgcca tgggcgaccg ctcctccggg ggtgacgagc aggccgaggc ccgcagcttc
     1741 tccaaggacc gctacttcca gggccacgtc gacgaggtcg ccaagctcgt gcccgagggc
     1801 atcgagggtc gggtcgccta caagggaccg gtcgccccgc tgatccacca gttcgccggc
     1861 ggcctgcggg cggccatggg ctactgcggc acccccacca tcgacgagct gcgcaccgag
     1921 agccagttcg tgcgcatcac ctcggccgcc ctccgcgagt cccaccccca cgacatcacg
     1981 atcaccaagg aagcgcccaa ctaccgggtg aagtaggcca ggcgcccacg gaccgaagcg
     2041 catcggctcg aacgggcggc cgggctcggc caccgcaacg tcgcgcggcg acggctgctc
     2101 agctgtccgg cgcgggcccc agcgtctcga gccatcgatc ggtctcggcg ggcgagcgga
     2161 gccggacgat ccgggggccc gccgggtcgg ccatccaccg gtcgatgtgc gctcgcttct
     2221 cccggaaggt gcggaagtgc caggcgatga tcgagtcgcg gctgagcgcg cggcgcagcg
     2281 tctcccggtt gccgttgcac acgagctcac gccggaccac ccggcgggcg gtccgccgca
     2341 gcagccggga cagcgaccgc caccgcgggt agtccagcgc cacgacgagc tcgaccctcg
     2401 ccatcaccag gtcccgccag cggctgtacg ccgtgtcgag gatccacgcg tcgccagacg
     2461 tgatccgctc ggcgatcttc ctctgctcgt cggtcgggcg ctcgacccat ccggggagcc
     2521 agccgagctc gtcgtcggcc gagtgccacg ggatcccggt gcgggctccg accgatcgag
     2581 ccaacgtcgt cttgccggac cccgtcacgc cgaagagcag gatccgtcgc gctgatccgc
     2641 ccggaaccga tgcctcgtcc tcgcccacga gccgagcctg ccaccggcgc ctcagccgcc
     2701 gccgggaagt caccgtgccg acgtgccagc catcagcaag gctgccccgg tgttctccct
     2761 cgacggacga cgtgcgctgg tcaccggcgc cagcacgggc atcggccggg ccatggccgg
     2821 tgcgctcgcc gatgccggcg ccgaggtcgt gctggtggcc cggacccgtg ccgacctggg
     2881 gcgagccgct gacgagatcg gcgaacgggc gacctggatc gccgcagacg tcggtcagga
     2941 ggcagagatc caccgcatct gcgagcacgt gggcgacgtc gacatcctcg tgaacgccgc
     3001 cggggtgaac ctccgaccgc cgatgcacga gctcaccacg gatgactggg ccatcacgat
     3061 ggcggtgaac ctcaccgcgc cgttcctgct cggccagtgc ctggcacccg gcatggtcga
     3121 gcgaggctgg ggccgcatcc tgcacgtcgg ctcgcagcag tcctggcggg cgttcgggaa
     3181 cagcggaggc tacggcgcct ccaaggccgg gctcctcggc ctgacccgct cccaggccga
     3241 ggcctggtcc cccgccggcg tcaccgtgaa ctgcgtcatc cccggcttcg tccgcacggc
     3301 catgaccgag cgcatcttcg tggaggagcc cgaacgggcc gaggcgctcg cggcccgcac
     3361 catggtcggt cgcaacggga cgccggagga cttcgccggc atcgcggtgt tcctgtgctc
     3421 cgacgcggcc gcgttcgtca ccgggcaggc catcgccgtc gacggcggct tctccgccac
     3481 ctgacgccta cggttgcgac gtggctgcct ccctccggcc ggtgctggat gtcctccgct
     3541 gccccgactg cggcgcagcg ctggagccat ccggtccgga cgccgtcacc tgccgcaccg
     3601 ggcaccgctt cgacctgatc gcccaccggg cgccggatct ggcccggacc gtggtccagc
     3661 gcgacacacc gggccagcga gccatgcggt tccggcccct tgtgtcgatc tacgagtcca
     3721 tctggcgccc gctgttcacc cgactcgccg gcggcaccga tcccgctgcg gagaccgacc
     3781 agctcatcga gtggctcgag cccacgccgg gtgcgaccgt cctcgacctg gcctgcgggc
     3841 cgggcaacac cacgcgccag ctggcggctg gcattcccgg cgcctccgtc gtcgggctcg
     3901 acctgtccgt gccgatgctc gcggaggcgg tccgggcgac gccggacagc tcccccatcg
     3961 ggttcgcccg ggtcgatgcc cacacgttgc cggtcggcga cgccaccgtc gacgtggtgc
     4021 actgcgccgc ggccctctac ctcttcggcg acccggctgc ggtcatcgcc gaggtggcgc
     4081 gggtcatccg cccgggaggc cggttcatcg gcatgaccct ggtcgcaccg ttgcagccgc
     4141 tcgggggcgt cggccgcagg gcggagcaga ccttcgcccg tctctcgggc ttgcggtact
     4201 tcggagcccg ggagctcgag gagatgtgcc gtgccgccgg cctggagggc ttctccgccc
     4261 ggtcgcaggg cggggcgctg ctgttcgtcg ccgacaggcc aggcggctga ccccgcggcg
     4321 cgactacccg acgaagtcgt cgtagctggc gtcgagctcg gcggtggagt actgccggaa
     4381 ggcgatcatc gtggtggtgt cgatcacgcc gtcgagcttg gagatgtgct ccgtgacgac
     4441 ggtggcgatg gcttcgtggt cgggcacggc cagcacggcc accagatcgg tccccgaccc
     4501 ggcgacggag tgggtctccc ggacaccgtc ggtgtcggcc agggcgtggc cgaggtcggt
     4561 gatggcgtcg ggacgggcct ggatgaggac gaaggcgacg atcacgtcgg cgaggctacg
     4621 cgcccggctc cggggccgcg accggccgcc ggggctggac agccgaaccg gtcaggcgca
     4681 tgctcgaagg agagtccgct cggcatccga gggccgagca tgacgagggg aggctgcgat
     4741 gcgccgctac ctggtcgtcg cccacaagac gctcggcggc gcccacctgg tcgagcacct
     4801 gcgcgagctg cgcgaggagc acccgggctg ccggttccac ctggtcgtcc cgatgcacca
     4861 cccggaccac gcgtggaccg agggcgaggt gcgcgccggg gcgaaccgga ccctggacga
     4921 gatgctcgag cggctggcgt ccatggggat gggcgccacc ggcgaggtcg gcgacgccaa
     4981 ccccgtctac gcggtcgggg tcgtcctgcg ccgcgagggt cccgacgcct tcgacggcat
     5041 cgtcctgtcc accctccccc acgggatctc ccggtggtgg cgcttcgacg tgcccagccg
     5101 gatgaccgcg gcgtacccag gcctcccgct cacccacctg gtgtcggacg aggcgctggt
     5161 gtgacgctcc gtcgacctct cacccggcag gggcggtgat gcgggtgagc aggtcgacga
     5221 gcatcgaggc ggtggcgccc cagatcgtgt cgccgacgat ctcgaagaag tggatgggcc
     5281 ggtcgatgcc gggcagcccc cagcgctcct ggcggtaggc ctccgggtcg aggagctcgg
     5341 ccagcggcac gtggaggatg gcgtccacct cgttcgggtt gggcaccagg cgggggcgct
     5401 cgggcagcac accgacgtac ggcacgatcg aggagcggct cgtgacggtg gcgaggtggt
     5461 ggagctcgcc gatctgctcg acggacgccg ggtcgagcgc gacctcctcg tcggcctcgc
     5521 ggagcgcggt ctgccacggg gtctcccccg gctcctgctt gccgcccggg aagctgactt
     5581 caccggtgtg ggagcgcatg tgccaggccc ggcgggtgag caccacgtgg gtctcaccgt
     5641 cgtgctcgta cagcggtgcg agcaccgccg agcaccggct ggtcagtccc tcgaccggcg
     5701 agcgacgggg ctcggggccc tccgccagcg cggcgcgcac ctggtcgagg ctcgggtggc
     5761 gcagctcctc gggcagcgac gcccacgacg gcggtccgcc gaggcgggtg cccggcggcc
     5821 gggggatgcg ctggggtccg ccgcggctca cgatcggcga gcgcaccggg gctgacgcac
     5881 cgtcagccga gcacgtcacc gacggtctca ccgtcgccgt cggagccggc gacgatgccc
     5941 tcgagcagct ggcgcatgcc gtgcatcttc agctgcttca tgagctcgcc cggggggatc
     6001 tcacccttct cccactccat ccagtggtcg aggagcttct cggggttcgg cggggggatc
     6061 tccatacggg gccgaggcta gccacgcgcc ggacgaaaac ggcgagtgcc gggccactcg
     6121 ggcccggcac ttcgctcgat ctgggttcag acggaagggc tcagaagccc atgccgccca
     6181 tgccacccat gccgcccatg ccaccggtgg ggtcgcccat cggggcgccc tcctcctcgg
     6241 gcttgtcggc gaccagggcc tcggtggtga gcaccatggc tgcgatggac gccgcgttct
     6301 gcagcgccgc gcgggtgacc ttggccgggt cgatgacgcc ggccttgacc aggtcgacgt
     6361 actcgccggt ggcggcgttg aagccgttgc cgccggtctc acgctcgatg gcctcgacca
     6421 cgacggcgcc ttccatgccg gcgttgacgg cgatctgacg ggccggggcg gccagcgcgg
     6481 tgtacacggc gcgggcgccg gtctgctggt cgtcctcgag gctgtcgatc accttctgca
     6541 ccgcggcgcg ggcccggagc agggcggtgc cgccaccggc gaccacgcct tcctcgatgg
     6601 ctgcacgggt cgccgagagg gcgtcctcga tgcggtgctt cttctccttg agctccacct
     6661 cggtggcggc gcccacgcgg accacggcga cgccgccgct gagcttggcg aggcgctcct
     6721 ggagcttctc gcgatcccag tcggagtcgg tgtcgtcgat ctcgcgcttg atctgctcga
     6781 tgcgaccggc cacgtcggcc tcggagccgc caccctcgac gatggtggtg tcgtccttgg
     6841 tgatgatcac cttgcgggcg gtgccgagca ggtcgagggt cgtgctctcg agcttgaggc
     6901 cgacctcttc ggagatgacc tggccaccgg tgaggatggc catgtcctgc agcatcgcct
     6961 tgcggcgctc gccgaagccg ggggccttga ccgccgcggc ggtgaaggtg ccgcggatct
     7021 tgttcacgac gagcacggcc agggcctcgc cctcgacgtc ctcggcgatg atcaccagcg
     7081 gcttgccgga ctgcatgacc ttctcgagga ccgggagcac gtcctggacc gaggagatct
     7141 tggagccgta gaggaggatg taggggtcct cgaggacggc ttcctggcgc tctgcgtcgg
     7201 agacgaagta cggggacagg tagcccttgt cgaactgcat gccctcggtg aactcgaggt
     7261 ccatgccgaa ggtgttggac tcctcgacgg tgaccacgcc gtccttgccg accttgtcga
     7321 tggcgtcggc gaggatctcg ccgatggtcg ggtcg
//
LOCUS       DATISU010000428        16398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_86152, whole genome shotgun sequence.
ACCESSION   DATISU010000428 DATISU010000000
VERSION     DATISU010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 16398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16398
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86152"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..739)
                     /locus_tag="VLR27_17015"
     CDS             complement(125..739)
                     /locus_tag="VLR27_17015"
                     /inference="COORDINATES: protein motif:HMM:NF015018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HSP05209.1"
                     /translation="MTIQHEDRPAGEPWKPRIRPVKFVVAGGFGAGKTTFVGALSEIP
                     PLRTEAAMTTVGARVDHRRGSEAKTTTTVAMDFGRLTVDEYLVMYLFGTPGQERFGFM
                     WDDICTHALGAVVLVDTDRLDQSFPAVDYFEAKDIPFVVAVNRFEGTVRHDLEAVRDA
                     LGVSAHVPMTECDARHTESAKQVLLLLLDELVNAARARAAATVV"
     gene            complement(747..1112)
                     /locus_tag="VLR27_17020"
     CDS             complement(747..1112)
                     /locus_tag="VLR27_17020"
                     /inference="COORDINATES: protein motif:HMM:NF017170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HSP05210.1"
                     /translation="MSAQSPSGPGRTGGHDVVRPFVMTGGRTRSERRDLRIETMLTAV
                     PGASTSGLSAEQVKLLRLCQAPISIAEASAELGLVIGVTTILAADLVSEGRLEVHQTD
                     PVEIELDQLSRMIDAVRAL"
     gene            complement(1109..1531)
                     /locus_tag="VLR27_17025"
     CDS             complement(1109..1531)
                     /locus_tag="VLR27_17025"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HSP05211.1"
                     /translation="MTMQDDVRQFSWMIDSFVEQTPGVTDAIAVSADGLLMAMSKSLD
                     RAGAEQLAAIVSGLVSLGHGTTSCFGFEQLEQVIVAMKGGYLFVSSISDGSCLGVVAS
                     KRCDIGQVGYQASLLVDRARAVLTPELILELQQEVLVS"
     gene            complement(1528..3963)
                     /locus_tag="VLR27_17030"
     CDS             complement(1528..3963)
                     /locus_tag="VLR27_17030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP05212.1"
                     /translation="MKFRIRSKLAAALAVPLVALVVMSGLQAVQAQEESDRIESEADR
                     AFVALGPGSVTTALQNERNYLSLDLIGLAGATTLEVSSADQAVEETDAAIAQLERAVT
                     GMEPQVQAAYAPAFEALDDLPAVRRAFDGYDGDKDLDNQELADEVFSAYTGMVASFFD
                     ATSVIATQVDEAALRNGVELVDAASRRSETIAAATRNVVLDTLTGGTSIDLRVQSIGL
                     IERLESLDARILQLSVDAYADVATETFARPETERYMEIFRGYVSGEDVDITELLANVG
                     TQEGVASIADLTTDALADQASTLSSDAYDEFRFFVLVAAGVIALAIIITYVATTSITR
                     PLRKLRDEADAMAGRRLPEAVRSILDTPFGDDVVIPELAPIEVKTRDEVGEVVEALNK
                     VQDRTLALAADQAVLRRNIADSFVNLGRRNQNLLDRQLEFITELEQLETEPDQLEALF
                     RLDHLATRMRRNAESLLVLAGTESPRQWGLPVDLDAVVRAGLGEVEDYRRVSVRSLED
                     AAIAGGAAAGVSHVVAELTENALQFSPPDEDVEIKGRRSHDGYVIAIADNGIGMSAED
                     LERANRRLSGEESYTVAPSKYLGHYVAGQLATRLGVTVRLQDNPAGGLIATLVVPPSL
                     LEDPVADEAPVPTTPAAETAPEQPADAAPAPEPVEEPQVDQPDVEVHAEADHHDAPTP
                     AGAPASLAEALGDGRLDSVETVADLERAFDGPAEPPAPPTLNGLPRRVPGAQRPDAAP
                     LAAHRRAVEDEKIPEPAPAPVEERPAAEAGSAGLFGFLAAFEDGAARAAQPGDPTTDA
                     TTEPVPGRTEEER"
     gene            complement(3960..5177)
                     /locus_tag="VLR27_17035"
     CDS             complement(3960..5177)
                     /locus_tag="VLR27_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05213.1"
                     /translation="MIRREPDLLHPRLTVVPVDVGPRRAVDHAPGRTVVGRDELEAAT
                     QAVAARRRTARLEQLAARNADLEGALERLAAARTAVDQRRADHDGFVSAAEWCETLPA
                     LAAAHRTDLAAAEAELSEALRHAREASRALDRVLEQRAQADAAISEARRQLHGLDRGE
                     GDPNGERAQAAAALSAQAESLEARLSEAERDARARSEETKRAVTAIERTIEGLVRDQR
                     DRQSRLTALVDCLPGDARPPHDDDPIQHVDAVSAGLRGLAATIAAELTGLTATLDRHR
                     AECEHRRSEVAEVRATLDRISPDDAAEALAGLVTGVAGDVVVLDDVVATDPDGVDDGM
                     LRALEAAEPDAPLVLLSSDPTVLGWAIDLPAERGALVGPRAVDLLDCPTTSTARPTAD
                     TAHAVSSSTGDPR"
     gene            complement(5312..6067)
                     /locus_tag="VLR27_17040"
     CDS             complement(5312..6067)
                     /locus_tag="VLR27_17040"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phosphotransferase"
                     /protein_id="HSP05214.1"
                     /translation="MPSWLAGPPADGAEPPPAVRRLAGDDPVRAVWENQVGGLTFAVG
                     APPTRYVKWLPRDVDPALADLAAEAERLRWARPHTPVPEVLDVGGDDEAAWLVTAALP
                     GTSAVDPRWIADPAPAVRAIGAGLRALHDALPVAGCPFDWSPAHRLGHLQPRFEHLRA
                     RLGDPPPPDRLVVCHGDACAPNTLVGADGAWIGHVDLGSLGVADRWADLAVASMSLGW
                     NHGPGWEDDLFEAYGIEPDPVRIDYHRLLWDAT"
     gene            complement(6072..7472)
                     /gene="zwf"
                     /locus_tag="VLR27_17045"
     CDS             complement(6072..7472)
                     /gene="zwf"
                     /locus_tag="VLR27_17045"
                     /inference="COORDINATES: protein motif:HMM:NF009492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HSP05215.1"
                     /translation="MATTEHSDAVVLFGATGDLAKKKLFPALYQMIVDGSLDDGVPVI
                     GVSSSDWSDDDLRERIAESVEDQVDDVDADALSSLQQRSSYVSGDYQEASTFDELKTV
                     LEGACDLRSPLFYLAIPPALFDDVVEGLAGVGLHEQARVVVEKPFGRDRASAQELDDT
                     LHGAFPEDRVFRIDHFLGKEAIENLLVFRFANSLLEPVWNRNFISSVQITMAEDFGVE
                     GRGKFYDSVGALKDVLQNHLLQIVAFLAMEPPAGPDARSLRDEKVKVFRQVEALQPDQ
                     LVRGQYEGYLDEDGVEAGSDTETFVAARFEIDSWRWAGVPWLIRTGKRMETTQTEAVV
                     ELRTPPSMLFAAEGSPAPHPNHIRFRLNSDTGVTFGLQVKQAGEDLVAEDADLEMSFS
                     HDERRREAYQRLLEDAMEGDARRFAREDGIDQQWRIVDDVLRDPPPAEHYAPGSWGPA
                     SADRLAADLGGWHHPG"
     gene            complement(7474..8397)
                     /gene="gnd"
                     /locus_tag="VLR27_17050"
     CDS             complement(7474..8397)
                     /gene="gnd"
                     /locus_tag="VLR27_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HSP05216.1"
                     /translation="MQLGMIGLGRMGANMAERLGAAGHDVIGFDPFSEASDVESLEAL
                     VAALDAPRTLWSMVPAGAPTTQTVNDLVELLEEGDVLVEGGNSRFTDSVIRAERYAEA
                     GLGYVDAGVSGGIWGLERGYALMVGGDDEHVQRVWPLLVDLAPEGAADTGKGLAHVGP
                     AGAGHFTKMVHNGVEYALMQAFAEGYELMARHPIGVDVPAALGAWQEGSVIQSWLLDL
                     LVRGLEAHPGFEDLDDVASDSGEGRWTVETAVATGVPVPAIAAALFARFESQHEESLA
                     MKAVAALREQFGGHAVVRSDDPRETPAGEGG"
     gene            8535..9062
                     /locus_tag="VLR27_17055"
     CDS             8535..9062
                     /locus_tag="VLR27_17055"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HSP05217.1"
                     /translation="MSPASTTTVESPFKKGDAVIYPQHGAGRVVAVEKRTIGDVERQY
                     LVIELELQDLRVWVPCDEAEQVGVREPTPKDELDDLFELLSDHDVRIPSNFSRRMKNN
                     QSRMNDGDVWQLAEVVRNLAIRRSKKHLSPSEKTLYAHARNLLVAELALSMGKSHEKA
                     EAAIDEHLPEPGEDG"
     gene            complement(9069..10325)
                     /gene="hemL"
                     /locus_tag="VLR27_17060"
     CDS             complement(9069..10325)
                     /gene="hemL"
                     /locus_tag="VLR27_17060"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HSP05218.1"
                     /translation="MTTNEELYAQAEARIPGGVNSPIRAFRAVGGTPYFVERAEGPRV
                     WDVEGTEYLDLVQSYGAVIAGHAHPAIVSAIQEAATKGTSYGAPTEGEVRIAEEICAR
                     VPSVEKVRLVSSGTEATMSAIRLARGHTGRTKLVKFAGNYHGHGDALLAQSGSALAVF
                     SLPESVGVTEATVADTIVVPFNEVPTLDEQVAAVILEPAAANMGLIPPDEGFLQALRD
                     ECDRVGALLIFDEVITGFRVARGGAQELYGVDADLTCFGKVIGGGLNIGAFGGRAEIM
                     DSMAPDGPVFQAGTLSGNPLATAAGRAALDLLDAAAYDRLRAGATRLADGLGKAFADA
                     GIDVVIPRVSSLVGLHLGDTAPRTYDDAKTTDVDRYRRFFHAMLRRGVAMAPGAYEVL
                     FPGLAHTDDVIDDIVARASEAAAEVA"
     gene            complement(10322..11305)
                     /gene="hemB"
                     /locus_tag="VLR27_17065"
     CDS             complement(10322..11305)
                     /gene="hemB"
                     /locus_tag="VLR27_17065"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HSP05219.1"
                     /translation="MAFPERRLRRLRRTPALRRMVAETQVGPDDLIAPLFIREGIDQP
                     NPIESLPGVVQHTVDSARREAAELTALGVPAVILFGVPATKDATGSQSCDPDGIVQVA
                     LRALKADLGDDLVLIADLCVDEYTDHGHCGIVRDDGTVDNDATLELYTMAAVAQARAG
                     ADIVAPSGMMDGQVAAIRYALDQDDHPEIPILAYAAKYASALYGPFRDAVDVTIAGGG
                     DRKGYQQDPANVRESMEEMEADLSEGADMLMVKPALAYLDVISRARTRFDVPLAAYHV
                     SGEYAMVKAAGERGWIDADAVMREHLLGIKRAGADLILTYAARQIAEEHAR"
     gene            complement(11323..12000)
                     /locus_tag="VLR27_17070"
     CDS             complement(11323..12000)
                     /locus_tag="VLR27_17070"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HSP05220.1"
                     /translation="MRDDVPPDAILFDFDGLVLDTETSEFLTVAESFADHGLELSRPD
                     WVAIIGTADHPHWMDMLEEALGRPLDDREAILETRRARHHARISDEPLRPGVIELATA
                     ATEAGIGVAIASSSPLDWVTGHLQRFDVDHLFPVKATRDDVGKERTKPHPDLFLLAAE
                     RLGVDPTRCVVLEDSEPGVTAANAAGCVTVGVPAGMTADAAFAHADLVVPSVAELDLE
                     RLAGLFG"
     gene            complement(11993..13450)
                     /gene="cobA"
                     /locus_tag="VLR27_17075"
     CDS             complement(11993..13450)
                     /gene="cobA"
                     /locus_tag="VLR27_17075"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HSP05221.1"
                     /translation="MTVYLVGAGPGDPGLLTVRGAELLARADVVVHDRLSAEELLDLA
                     PARAVRIDVGKSPGHARVPQGGINELLVEHGRTGATVVRLKGGDPTVFARGGEEAMAL
                     ADAGIPFEFVPGITSAIAVPAYAGIPVTQRFSSTSFTVVTGHEDPSIGDEGSVDWDAV
                     ARVGGTIVLLMGVGRIAKICDRLMAAGRPADTPAAAVIWGTRANQRTVRATLGTLAAQ
                     DLESPATIVIGAVAGLDLAWFERRPLFGRTVVVTRARAQASSLVARLHELGAATVEIP
                     AIRIDDPADGGAALRAAVADLAGNDWVVVTSPNGARRLLAECRDARAFGAAKVAAIGP
                     GTADALRAGNVEADLVPERFVAEGLLEVFPDPPAAGGRVLLARAAEARDVLPETLRER
                     GWDVDVVEAYRTVQGTPSPEELAAAAEADVVTFTSSSTVRNFLAVCDTVPPVVACIGP
                     VTADTAREHGLTVDVEAEVHTIDGLLAALTAHFDA"
     gene            13498..14184
                     /locus_tag="VLR27_17080"
     CDS             13498..14184
                     /locus_tag="VLR27_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05222.1"
                     /translation="MADEERFEEVVDPVDGTRWRIDVGFLESSWTCLWGDGCQGILDE
                     PAAHLMQGCCSVGAEMLDEDEAMLVAALGATLEPEQLQHHAEAVEHGFFSDDTRTNTR
                     VVDGACIFFNRPGFPGGVGCALHRAAEAAGEPVIDWKPEVCWQLPLRIDRDGDTATLR
                     RWSRADWGSDGDTMHWCCTERPSGPDQADAYVGDRPVIESLAEEIEALVGTEVLVEIT
                     RRGGRRPAGG"
     gene            complement(14147..15070)
                     /gene="hemC"
                     /locus_tag="VLR27_17085"
     CDS             complement(14147..15070)
                     /gene="hemC"
                     /locus_tag="VLR27_17085"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HSP05223.1"
                     /translation="MKLRAATRSSPLARWQTEHVIALLEAAHPGIEVEPVLLTSAPDK
                     DLTTPISALGGKGLFAKEVQEAVLDGRADFAVHSAKDLPSQTVPGLTLASVPERGDPR
                     DVLVGAALVDLPVGAVIATGSNRRRVQLQDLRPDLRFVELRGNIQRRVATAGTKIDDG
                     ELAGSRITAIIAAGAAMLRQDMEADITDWLPAYVMTPQVGQGALGIECRADDDATRDL
                     LAAIEHAPSRRRVDAERSFLVALGGDCDLPAGAHATLTDDDPASEITLRMVIGADDGS
                     RLERAVIRGTDAATIGAEGITLLPVADLLGG"
     gene            complement(15126..>16398)
                     /locus_tag="VLR27_17090"
     CDS             complement(15126..>16398)
                     /locus_tag="VLR27_17090"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:NF000744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HSP05224.1"
                     /translation="EIMPVVVIGVNHRTAPLGVLEAMTVGDAALGKTLHALLTKEHVS
                     EAVVLSTCNRTEVYAVAEKFHGGYADVRAVLAELAFLDAEDFADHLYVHHDTAAVAHL
                     FDVVSGLDSAVLGEHEIQGQVKISWEKAQREGAAGTTLNLLFRHALEAGKRARSETGI
                     GQGTASVSYAAVALAAQQLGNLDGRRAIVLGAGEMGSSMAESLASAGVAELIVANRTH
                     DKAKALAAAVGGRAIRFADLTDELVDADVVLTSTGAASVLLTRDDVEVAMAARNGQPL
                     VLVDIAVPRDIEPSVAELAGVTVLDMDDLGAFADRGLAERRREVDAVRAILDEELERY
                     RSATSAREIAPVIVALREGAEALRAAELERARARLTELDDAQIAAVEKLTQGIVAKLL
                     HQPTVALRDAAGTTKGDRLVQALRDLYDIDS"
BASE COUNT         2120 a         5571 c         6488 g         2219 t
ORIGIN      
        1 cgcccccgag aacggcaccg gccccgcctc tgcaggcggg gccggttcgc gtccggggac
       61 gctccgagcg atggttccgg gccgcggcgc acgcctcgac ggcgcgcctc gcagcccgga
      121 tcgatcagac gacggtggcg gcggcccggg cccgggccgc gttgaccagc tcgtcgagca
      181 ggagcagcag cacctgcttg gccgactcgg tgtggcgggc gtcgcactcc gtcatcggca
      241 cgtgggcgct gacgccgagg gcgtcccgca cggcctcgag gtcgtggcgg accgtgccct
      301 cgaagcggtt gaccgcgacg acgaagggga tgtccttggc ctcgaagtag tccacggcgg
      361 ggaagctctg gtcgagccgg tcggtgtcga ccagcacgac ggcgccgagg gcgtgggtgc
      421 agatgtcgtc ccacatgaag ccgaagcgct cctggccggg cgtgccgaac aggtacatga
      481 ccaggtactc gtcgacggtg agccggccga agtccatggc cacggtggtc gtggtcttgg
      541 cctccgagcc gcggcggtgg tcgacgcggg cgccgacggt ggtcatcgcc gcctcggtcc
      601 gcaggggcgg gatctccgac agcgcaccga cgaaggtggt cttgccggcg ccgaagcccc
      661 cggcgaccac gaacttcacc gggcggatgc gcggcttcca gggctccccg gcggggcggt
      721 cctcgtgctg gatcgtcatg gttctcctac agggctcgca cggcgtcgat catgcgcgac
      781 agctggtcga gctcgatctc cacgggatcg gtctggtgca cctcgaggcg gccctcggag
      841 acgaggtcgg cggcgaggat ggtggtgacg ccgatgacca ggccgagctc ggcggaggcc
      901 tcggcgatgg agatcggcgc ctggcagagc cggagcagct tgacctgctc ggccgacagc
      961 cccgaggtcg aggcgccggg gacggccgtc agcatcgtct cgatgcgcag gtcgcgccgc
     1021 tcggaccgcg tccgcccgcc cgtcatcacg aacggtcgga cgacgtcgtg gcccccggtg
     1081 cgcccgggcc ccgacggcga ctgggcgctc atgacacgag gacctcttgc tggagctcga
     1141 ggatgagctc cggcgtgagc acggcgcggg cccggtccac gagcaggctg gcctggtagc
     1201 cgacctggcc gatgtcgcag cgcttggagg ccacgacgcc gaggcagctg ccgtcggaga
     1261 tcgaggacac gaacaggtag ccgcccttca tggcgacgat gacctgctcg agctgctcga
     1321 acccgaagca cgacgtggtg ccgtggccca ggctcacgag gccggacacg atggcggcga
     1381 gctgctcggc gcccgcccgg tccagggact tggacatggc catcagcagc ccgtcggccg
     1441 acacggcgat ggcgtcggtg acgccgggtg tctgctcgac gaagctgtcg atcatccacg
     1501 agaactggcg gacgtcgtcc tgcatggtca tcgctcctcc tcggttcgcc cggggacggg
     1561 ctcggtggtc gcatcggtgg tggggtcgcc aggctgggcg gcacgggccg cgccgtcctc
     1621 gaaggcggcg aggaagccga acaggccggc gctgcccgcc tcggcggcgg ggcgctcctc
     1681 gaccggcgcg ggtgccggct ccgggatctt ctcgtcctcg acggcccggc ggtgggcggc
     1741 gaggggggcg gcgtcggggc gctgggcgcc ggggacccga cggggcagcc cgttcagggt
     1801 cgggggtgcc ggcggctcgg ccgggccgtc gaaggcccgc tcgaggtcgg cgacggtctc
     1861 gacggagtcg agccggccat cgccgagcgc ctcggccagg ctggcggggg caccggccgg
     1921 ggtcggggca tcgtggtggt ccgcctcggc gtggacctcg acgtcgggct ggtccacctg
     1981 gggctcctcc acgggttcgg gtgcaggcgc cgcgtcggcg ggctgctcgg gggcggtctc
     2041 ggccgccggg gtcgtcggca cgggggcctc gtcggcgacg gggtcctcca gcaggctggg
     2101 cggcaccacg agcgtggcga tgaggccgcc ggccgggttg tcctggaggc gcacggtgac
     2161 gccgaggcgg gtggccagct ggccggcgac gtagtggccg aggtacttgg acggggcgac
     2221 cgtgtaggac tcctcgccgg agagccggcg gttggcccgc tcgaggtcct cggcgctcat
     2281 cccgatgccg ttgtcggcga tggcgatcac gtagccgtcg tgggagcggc ggcccttgat
     2341 ctccacgtcc tcgtcgggcg gggagaactg cagggcgttc tcggtgagct ccgccacgac
     2401 gtgggacacg ccggcggcgg cgccaccggc gatggcggcg tcctcgaggc tgcggaccga
     2461 cacccggcgg tagtcctcga cctcgccgag gccggcgcgg acgacggcgt cgaggtcgac
     2521 cggcaggccc cactggcgag ggctctccgt gccggccagc acgaggagcg actcggcgtt
     2581 gcggcgcatg cgggtggcga ggtggtcgag gcggaacagc gcctcgagct ggtcgggctc
     2641 ggtctcgagc tgctcgagct cggtgatgaa ctcgagctgg cggtcgagca ggttctggtt
     2701 gcgccggccc aggttgacga aggagtcggc gatgttgcgg cgcagcacgg cctggtcggc
     2761 ggcgagggcc agcgtgcggt cctggacctt gttgagggcc tcgacgacct cgccgacctc
     2821 gtcccgggtc ttgacctcga tgggggcgag ctcggggatc acgacgtcgt cgccgaaggg
     2881 cgtgtcgagg atcgagcgca cggcctcggg caggcggcgg ccggccatgg cgtcggcctc
     2941 gtcccgcagc ttgcgcagcg gccgggtgat cgacgtggtg gcgacgtagg tgatgatgat
     3001 ggccagggcg atgacgccgg cggccacgag gacgaagaac cggaactcgt cgtaggcgtc
     3061 gctggacagg gtgctggcct ggtcggccag ggcatcggtg gtgaggtcgg cgatgctggc
     3121 cacgccctcc tgggtgccca cgttggcgag cagctcggtg atgtcgacgt cctcgccgct
     3181 gacgtagccc cggaagatct ccatgtaccg ctcggtctcg gggcgggcga aggtctcggt
     3241 ggcgacgtcc gcgtaggcgt cgacggacag ctgcaggatg cgggcgtcga gcgactcgag
     3301 gcgctcgatg aggccgatgg actggacccg gaggtcgatc gacgtgccgc cggtcagggt
     3361 gtcgagcacg acgttgcggg tggcggcggc gatggtctcg ctgcggcggc tggcggcgtc
     3421 gacgagctcg acgccgttgc gcagcgcggc ctcgtcgacc tgggtggcga tgacggaggt
     3481 ggcgtcgaag aacgaggcga ccatccccgt gtaggcggag aagacctcgt cggcgagctc
     3541 ctggttgtcc aggtccttgt cgccgtcgta gccgtcgaag gcccggcgca cggcgggcag
     3601 gtcgtcgagg gcctcgaagg ccggggcgta ggccgcctgc acctggggct ccatgccggt
     3661 cacggcccgc tcgagctggg cgatggcggc gtcggtctcc tcgaccgcct ggtcggccga
     3721 gctgacctcg agggtggtgg ccccggccag gccgatgagg tcgagcgaca ggtagttgcg
     3781 ctcgttctgg agggcggtgg tgacgctgcc cgggccgagg gcgacgaatg cccggtcggc
     3841 ctcggactcg atgcggtcgg actcctcctg ggcctgcacg gcctggaggc ccgacatgac
     3901 cacgagggcc acgaggggca cggccagggc ggcggccagc ttggatcgga tgcggaactt
     3961 catcgtgggt ctcccgttga cgagctgacg gcgtgtgcgg tgtcggcggt gggccgtgcg
     4021 gtggatgtgg tggggcagtc gaggaggtcg acggcgcggg ggccgacgag ggcgccccgc
     4081 tcggcgggca ggtcgatggc ccagccgagg acggtggggt cggagctgag gaggacgagg
     4141 ggtgcgtcgg gctcggcggc ctcgagggcg cgcagcatgc cgtcgtcgac gccgtcggga
     4201 tccgtggcga ccacgtcgtc gaggaccacg acgtcgccgg cgaccccggt gacgaggccg
     4261 gcgagggcct cggctgcgtc atcgggcgag atgcggtcga gcgtggcgcg gacctcggcg
     4321 acctcgctgc ggcggtgctc gcactcggcc cggtgccggt cgagcgtggc ggtcagtccg
     4381 gtcagctcgg ccgcgatcgt cgcggcgagg ccccgcaggc cggcggacac cgcgtccacg
     4441 tgttggatcg ggtcgtcgtc gtggggtggt cgggcgtcac cggggaggca gtcgaccagg
     4501 gcggtgagcc gggactggcg gtcgcgctgg tcgcggacga ggccctcgat ggtgcgctcg
     4561 atcgcggtga cggcgcgctt ggtctcctcg gaccgggccc gggcgtcgcg ctcggcctcg
     4621 ctgaggcggg cctcgaggct ctcggcctgg gcggacaggg cggcggccgc ctgggcccgc
     4681 tccccgttcg ggtcaccctc gccgcggtcg aggccgtgga gctggcggcg ggcctcggag
     4741 atggcggcgt cggcctgggc ccgctgctcg agcacgcggt ccagcgcccg ggaggcctcg
     4801 cgggcgtggc gcagcgcctc ggacagctcg gcttccgccg cagcgaggtc ggtgcggtgg
     4861 gcggcggcga gcgccgggag ggtctcgcac cactcggccg cggagacgaa gccgtcgtgg
     4921 tcggctcggc gctggtcgac ggcggtgcgg gccgcggcca ggcgctcgag ggcgccttcg
     4981 aggtcggcgt tgcgggcggc gagctgctcg aggcgggcgg tgcgccggcg ggccgccacc
     5041 gcctgggtgg ccgcttcgag ctcgtcgcgc ccgacgaccg tgcggcccgg ggcgtggtcc
     5101 acggcgcgac gaggaccgac gtccacgggc accaccgtga ggcgggggtg caacaggtcg
     5161 ggctcgcgtc ggatcacatc tctccctggg actgcccggt ccggccagcg gctcgccggg
     5221 ggcggctctg agcgtcatcg gcagcgcgac ccggagactt gagcgccggc ggcggccgcg
     5281 gccgcgtatg ggccggacga cccaggacgg ctcaggtcgc gtcccagagc agccggtggt
     5341 agtcgatgcg gaccgggtcg ggctcgatgc cgtacgcctc gaagaggtcg tcctcccagc
     5401 cgggtccgtg gttccacccc aggctcatgg acgccacggc gaggtccgcc cagcggtcgg
     5461 cgacgccgag cgagccgagg tcgacgtggc cgatccacgc gccgtcggca cccaccaacg
     5521 tgttcggggc gcaggcgtcg ccgtggcaga cgacgaggcg gtcgggcggg ggcgggtcgc
     5581 cgagccgggc tcgcaggtgc tcgaagcggg gctgcaggtg cccgagccgg tgcgcgggcg
     5641 accagtcgaa cgggcatccc gccaccggga gggcgtcgtg cagggcgcgc aggccggccc
     5701 cgatggcgcg caccgctggg gccggatcgg cgatccagcg ggggtccacg gcgctggtgc
     5761 cgggcagcgc cgccgtcacg agccaggcgg cctcgtcgtc gccaccgaca tcgagcacct
     5821 cggggaccgg ggtgtgcggg cgcgcccagc ggagccgctc ggcctcggcg gcgaggtcgg
     5881 cgagcgcagg gtccacgtca cgggggagcc acttcacgta ccgggtcggc ggtgcaccga
     5941 ccgcgaacgt cagaccgccg acctggttct cccacaccgc ccggaccggg tcgtcaccgg
     6001 cgaggcgacg cacggccggc ggcggctcgg ccccgtccgc cggtggtccg gccagccagc
     6061 tcggcaccgg gtcagcccgg gtggtgccag ccgccgaggt ccgcagcgag gcgatcggcg
     6121 ctggccggcc cccagctgcc gggggcgtag tgctcggccg gcggcgggtc gcgcagcacg
     6181 tcgtcgacga tgcgccactg ctggtcgatg ccgtcctcgc gcgcgaaccg gcgggcgtcg
     6241 ccctccatcg catcctcgag cagccgctgg taggcctccc gccggcgctc gtcgtgggag
     6301 aacgacatct ccaggtcggc gtcctcggcc acgaggtcct cgcctgcctg cttcacctgg
     6361 agcccgaagg tcacgccggt gtcgctgttg agccggaacc ggatgtggtt ggggtgcggc
     6421 gccggtgacc cctcggcggc gaagagcatg ctcggcggcg tgcgcagctc gaccacggcc
     6481 tcggtctggg tggtctccat ccgcttgccg gtgcggatga gccacggcac cccggcccac
     6541 cgccaggagt cgatctcgaa gcgcgccgcc acgaaggtct cggtgtcgct gccggcctcg
     6601 acgccgtcct cgtcgaggta gccctcgtac tggccccgga cgagctgatc gggctggagc
     6661 gcctcgacct ggcggaacac cttcaccttc tcgtcccgga gcgagcgggc gtcggggccg
     6721 gccgggggct ccatcgccag gaaggccacg atctgcagga ggtggttctg caggacgtcc
     6781 ttcagggcac cgaccgagtc gtagaacttg ccgcgcccct cgacgccgaa gtcctcggcc
     6841 atggtgatct gcaccgagga gatgaagttg cggttccaga ccggctcgag cagcgagttg
     6901 gcgaaccgga acacgagcag gttctcgatg gcctccttgc cgaggaagtg gtcgatgcgg
     6961 aacacccggt cctcggggaa cgcgccgtgg agggtgtcgt ccagctcctg ggccgaggcc
     7021 cggtcgcggc cgaagggctt ctcgacgacg acgcgcgcct gctcgtggag cccgacgccg
     7081 gcgaggccct cgaccacgtc gtcgaacagg gcgggtggga tggccaggta gaagagcggt
     7141 gagcggaggt cgcacgcgcc ctcgaggacg gtcttcagct cgtcgaaggt cgaggcctcc
     7201 tggtagtcgc ccgagacgta ggacgaccgc tgctgcaggg agctgagggc gtcggcatcg
     7261 acgtcgtcga cctggtcctc caccgactcc gcgatgcgtt cgcggaggtc gtcgtcgctc
     7321 cagtccgagg agctgacccc gatgaccggg acgccgtcgt cgagcgagcc gtcgacgatc
     7381 atctggtaca gcgccgggaa cagcttcttc ttcgccaggt cgccggtggc gccgaagagg
     7441 accaccgcgt cggagtgctc cgtggtcgcc acctcagccc ccctcccccg ccggggtctc
     7501 gcgagggtcg tcgctgcgca ccaccgcgtg gccgccgaac tgctcgcgca gggcggcgac
     7561 cgccttcatg gcgagggact cctcgtgctg ggactcgaag cgggcgaaca gcgcggccgc
     7621 gatggccggg acgggcacgc cggtggcgac cgcggtctcc acggtccagc gcccctcgcc
     7681 ggagtcggat gcgacgtcgt cgaggtcctc gaagccgggg tgggcctcga ggccgcgcac
     7741 cagcaggtcg agcagccacg actggatgac cgagccctcc tgccacgccc ccagggccgc
     7801 cgggacgtcg acgccgatgg ggtggcgcgc catgagctcg tagccctcgg cgaaggcctg
     7861 catgagcgcg tactcgacac cgttgtggac catcttcgtg aagtggccgg caccggccgg
     7921 tccgacgtgg gccaggccct tgccggtgtc ggcggcgccc tcgggagcga ggtcgacgag
     7981 caggggccac actcgctgca cgtgctcgtc gtcgccgccg accatgagcg cgtagccgcg
     8041 ctcgaggccc cagatgccgc ccgacacgcc ggcgtcgacg tagccgagcc cggcctcggc
     8101 gtagcgctcg gcccggatca cggagtcggt gaagcgggag ttgccgccct cgaccagcac
     8161 gtcgccctcc tcgagcagct ccacgaggtc gttcaccgtc tgggtcgtcg gggcgccggc
     8221 gggcaccatc gaccacaggg tccggggcgc atccagcgcg gcgaccaggg cctcgaggga
     8281 ctcgacgtcg ctggcctcgc tgaacgggtc gaagccgatc acgtcgtgcc cggcggcgcc
     8341 cagccgctcg gccatgttcg cccccatccg tccgagtccg atcatcccga gctgcatgcg
     8401 tgcgtctcct ggccagtccg tggggcgtgg tcgtgccgcc ccttcctacc cgcggatgtt
     8461 ggttttcgcc gttcgagccg ccatggtggg agatgggcat gttctgcccc gagtcgcgcc
     8521 agaggagccc caacgtgtcg ccagcgtcca ccaccaccgt cgaatcgccc ttcaagaagg
     8581 gcgacgctgt catctacccc cagcacgggg ccgggcgggt cgtggcggtc gagaagcgga
     8641 cgatcggcga cgtcgagcgc cagtacctgg tgatcgagct cgagctgcag gacctgcgcg
     8701 tgtgggtgcc ctgcgacgag gcggaacagg tcggcgtgcg cgagccgacc cccaaggacg
     8761 agctcgacga cctgttcgag ctgctgtccg accacgacgt gcgcatcccg tccaacttct
     8821 cccggcgcat gaagaacaac cagagccgca tgaacgacgg cgacgtgtgg cagctggccg
     8881 aggtggtccg gaacctggcg atccgccggt ccaagaagca cctctccccc tccgagaaga
     8941 cgctctacgc ccacgcccgc aacctgctgg tcgccgagct ggccctctcg atgggcaagt
     9001 cccacgagaa ggccgaggcg gccatcgacg agcacctgcc cgagccgggc gaggacggct
     9061 agcgagggct acgccacctc ggcggcggcc tcggatgccc gggccacgat gtcgtcgatg
     9121 acgtcgtcgg tgtgggccag gcccgggaac agcacctcgt aggcacccgg cgccatcgcc
     9181 acgccgcgcc ggagcatggc gtggaagaac cggcggtagc ggtcgacgtc ggtcgtcttg
     9241 gcgtcgtcgt aggtgcgcgg cgcggtgtcc cccaggtgga ggccgacgag cgagctcacc
     9301 cgcgggatga cgacgtcgat gccggcgtcg gcgaaggcct tgccgaggcc gtcggccagg
     9361 cgggtggccc cggcgcggag gcggtcgtag gcggcggcgt cgagcaggtc gagcgcggcg
     9421 cggccggcgg cggtggccag cgggttgccc gagagcgtgc cggcctggaa caccggcccg
     9481 tccggtgcca tcgagtccat gatctcggcc cggccgccga acgcgccgat gttgaggccg
     9541 ccgccgatca ccttgccgaa gcaggtcagg tcggcgtcca cgccgtagag ctcctgggcg
     9601 ccgccgcggg cgacgcggaa gccggtgatg acctcatcga agatgagcag ggcaccgacc
     9661 cggtcgcact cgtcgcgcag cgcctgcagg aagccctcgt ccggcgggat cagacccatg
     9721 ttggcggcgg cgggctccag gatgacggct gcgacctgct cgtcgagggt gggcacctcg
     9781 ttgaacggga ccacgatggt gtcggccacc gtcgcctcgg tgacgccgac gctctcgggc
     9841 agggagaaga cggctagcgc cgagccggac tgggccagca gggcgtcgcc gtggccgtgg
     9901 tagttgccgg cgaacttcac cagcttcgtc cggccggtgt ggccccgcgc caggcggatc
     9961 gccgacatgg tcgcctcggt gccggagctg acgaggcgga ccttctccac gctcgggacc
    10021 cgggcgcaga tctcctcggc gatgcggacc tcgccctcgg tgggcgcgcc gtaggaggtg
    10081 cccttcgtgg ccgcctcctg gatggccgag acgatggccg ggtgggcgtg gccggcgatg
    10141 acggcgccgt agctctggac caggtcgagg tactcggtgc cctcgacgtc ccacacccgg
    10201 ggtccctcgg cccgctcgac gaagtacggc gtgccaccca cggcccggaa ggcgcggatc
    10261 ggcgagttga ccccgccggg gatgcgggcc tcggcctggg cgtagagctc ctcgttggtg
    10321 gtcatcgggc gtgctcctcg gcgatctggc gggcggcgta ggtgaggatg aggtcggccc
    10381 cggcgcgctt gatgccgagc aggtgctcgc gcatgacggc atcggcgtcg atccagcccc
    10441 gctcgccggc ggccttcacc atcgcgtact cgccggacac gtggtaggcg gcgaggggga
    10501 cgtcgaagcg ggtgcgagcc cggctgatca cgtcgaggta ggccagggcg ggcttgacca
    10561 tgagcatgtc ggcgccctcg gacaggtcgg cctccatctc ctccatggac tcacggacgt
    10621 tggccgggtc ctgctggtag cccttgcggt cgccgccgcc ggcgatggtg acgtcgaccg
    10681 cgtcccggaa cgggccgtac agcgcggagg cgtacttggc cgcgtaggcg aggatcggga
    10741 tctcggggtg gtcgtcctgg tcgagggcgt agcggatggc ggcgacctgg ccgtccatca
    10801 tccccgaggg ggccacgatg tcggcgccgg cgcgggcctg ggcgacggcg gccatcgtgt
    10861 agagctcgag ggtggcgtcg ttgtcgaccg tgccgtcgtc gcggacgatg ccgcagtggc
    10921 cgtggtcggt gtactcgtcg acgcagaggt cggcgatgag cacgaggtcg tcgccgaggt
    10981 cggccttcag cgcccgcagc gcgacctgca cgatgccgtc ggggtcgcag gactggctgc
    11041 cggtggcgtc cttggtggcc gggacgccga agaggatgac ggccgggacc ccgagggcgg
    11101 tgagctcagc ggcttcgcgc cgggcgctgt cgacggtgtg ctggaccacg ccgggcagcg
    11161 actcgatcgg gttgggctgg tcgatgccct cccggatgaa gagcggggcg atcaggtcgt
    11221 ccggccccac ctgggtctcg gcgaccatgc ggcggagcgc cggggtgcgt cggaggcggc
    11281 ggaggcgtcg ctcggggaag gccacggggc gagcctatgg cgtcacccga acaggccggc
    11341 caggcgctcc aggtcgagct cggccaccga gggcaccacc aggtcggcgt gggcgaaggc
    11401 cgcatcggcg gtcatgccgg ccggcacccc gaccgtgacg cagccggctg cattcgccgc
    11461 ggtgaccccg ggctcgctgt cctcgagcac cacgcagcgg gtggggtcga ccccgaggcg
    11521 ctcggcggcg agcaggaaca ggtccgggtg gggcttggtg cgctccttgc cgacgtcgtc
    11581 gcgcgtcgcc ttcacgggga agaggtggtc gacgtcgaag cgctgcaggt ggccggtgac
    11641 ccagtcgagc gggctggagc tggcgatggc cacgccgatg ccggcctcgg tcgccgccgt
    11701 cgccagctcg atgaccccgg gacgcaacgg ctcgtcgctg atgcgggcgt ggtggcgggc
    11761 ccggcgggtc tccaggatcg cctcccggtc gtccagcggc cggcccagcg cctcctccag
    11821 catgtccatc cagtgcgggt ggtcggccgt gccgatgatc gccacccagt ccggccggga
    11881 cagctcgagg ccgtggtcgg cgaagctctc ggccacggtc aggaactccg aggtctcggt
    11941 gtcgagcacc agcccgtcga agtcgaacag gatcgcatcg ggtggcacgt cgtcacgcat
    12001 cgaagtgggc ggtgagggca gcgaggaggc cgtcgatggt gtggacctcg gcctcgacgt
    12061 ccacggtgag gccgtgctcc cgggcggtgt cggcggtgac ggggccgatg caggccacga
    12121 cgggcgggac ggtgtcgcac acggcgagga agttccggac cgtggacgac gaggtgaacg
    12181 tgacgacgtc ggcctcggcc gcggcggcga gctcctcggg cgacggggtg ccctggacgg
    12241 tgcggtaggc ctcgacgacg tccacgtccc agccccgctc gcgcagggtc tcgggcagca
    12301 cgtcgcgggc ctcggcggcg cgggccagca gcacccggcc accggcggca ggcgggtcgg
    12361 ggaagacctc gagcagaccc tcggccacga agcgctcggg gacgaggtcg gcctcgacgt
    12421 tgcccgcccg cagcgcgtca gccgtgccgg gaccgatggc cgccaccttc gctgcaccga
    12481 aggcccgggc gtcgcggcac tcggcgagca ggcggcgggc gccgttcggc gaggtcacga
    12541 ccacccagtc gttgccggcc aggtcggcga cggccgctcg gagggcggcg cccccgtccg
    12601 ccgggtcgtc gatgcggatg gccgggatct cgaccgtcgc cgccccgagc tcgtggaggc
    12661 gggcgaccag cgacgacgcc tgggcccggg cgcgggtgac caccaccgtg cgaccgaaca
    12721 gggggcggcg ctcgaaccag gcgaggtcga gcccggcgac cgcgccgatc acgatcgtcg
    12781 ccggcgactc caggtcctgc gcggccagcg tgccgagggt cgcccgcacc gtgcgctggt
    12841 tggcgcgggt gccccagatg acggcggcgg cgggggtgtc ggccggccga ccggcagcca
    12901 tcaggcggtc gcagatcttg gcgatccggc ccacccccat gagcaagacg atggtgccgc
    12961 cgacccgggc gaccgcatcc cagtcgacgg agccctcgtc gccgatggag gggtcctcgt
    13021 ggccggtgac gacggtgaag ctggtggagg agaaccgttg ggtgacgggg atgccggcgt
    13081 aggcgggcac ggcgatggcc gaggtgatgc cggggacgaa ctcgaagggg atgccggcgt
    13141 cggcgagcgc catggcctcc tcgcccccgc gggcgaacac cgtcgggtcg ccgcccttga
    13201 ggcgcacgac cgtcgcgccc gtgcggccgt gctccacgag cagctcgttg atgcccccct
    13261 gcgggacgcg agcgtgtccc ggcgacttgc ccacgtcgat gcgcacggca cgggccgggg
    13321 ccagatcgag cagctcctcg gcagacaggc ggtcgtggac cacgacgtcg gcccgggcga
    13381 gcagctccgc gccccgcacg gtcagcaggc cgggatcacc cgggccggcg ccgacgaggt
    13441 acacggtcat gcgtcgcacc ttacgagcgc acgcccaggt gcccgtagcc tggggcggtg
    13501 gcagacgagg agcgcttcga agaggtggtc gaccccgtcg acggcacccg ctggcgcatc
    13561 gacgtcggct tcctggagtc gagctggacc tgcctgtggg gcgacggctg ccagggcatc
    13621 ctcgacgagc cggccgccca cctgatgcag gggtgctgct cggtgggtgc cgagatgctg
    13681 gacgaggacg aggcgatgct ggtcgccgcc ctcggcgcca cgctcgagcc cgagcagctc
    13741 cagcaccacg ccgaggccgt cgagcacggg ttcttctccg acgacacccg caccaacacc
    13801 cgggtcgtcg acggcgcctg catcttcttc aaccggcccg gcttccccgg tggcgtcggt
    13861 tgcgccctgc accgggcggc cgaggcggcg ggcgagccgg tgatcgactg gaagcccgag
    13921 gtgtgctggc agctgccgct gcgcatcgac cgggacggcg acaccgccac cctgcggcgc
    13981 tggagccggg ccgactgggg ctccgacggc gacaccatgc actggtgctg caccgagcgg
    14041 ccgagcggcc ccgaccaggc cgacgcctac gtcggcgacc gccccgtcat cgagtcgctg
    14101 gccgaggaga tcgaggcgct cgtcggcacc gaggtgctgg tggagatcac ccgccgagga
    14161 ggtcggcgac cggcaggagg gtgatgccct cggcgccgat ggtggcggcg tcggtgcccc
    14221 ggatgacggc ccgctcgagg cgggagccgt cgtcggcgcc gatcaccatg cgcagcgtga
    14281 tctcgctggc cgggtcgtcg tcggtgaggg tggcgtgggc gccggcagga aggtcgcagt
    14341 cgccgccgag ggcgaccagg aacgaccgct cggcgtccac ccggcgccgg ctgggtgcgt
    14401 gctcgatggc ggcgagcagg tcgcgggtgg cgtcgtcgtc ggcccggcac tcgatgccga
    14461 gggcgccctg gccgacctgg ggggtcatca cgtaggcggg cagccagtcg gtgatgtcgg
    14521 cctccatgtc ctggcggagc atggcggcgc cggcggcgat gatcgccgtg atccgcgagc
    14581 cggcgagctc gccgtcgtcg atcttcgtgc cggcggtggc gacccggcgc tggatgttgc
    14641 cccgcagctc cacgaagcgc aggtccggtc gcaggtcctg cagctgcacc cggcggcggt
    14701 tggagccggt ggcgatcacc gcgccgaccg gcaggtcgac gagggcggcg ccgacgagga
    14761 cgtcgcgggg atcgccccgc tcggggacgg acgccagcgt caacccgggg acggtctggg
    14821 acggcaggtc cttggccgag tggacggcga agtcggcccg gccgtcgagc accgcctcct
    14881 ggacctcctt ggcgaacagg cccttgccgc cgagcgcgga gatcggggtg gtcaggtcct
    14941 tgtccggcgc ggaggtgagc agcacgggct cgacctcgat gccggggtgg gccgcctcga
    15001 gcagggcgat gacgtgctcg gtctgccacc gggccagcgg cgacgagcgg gtggcggcgc
    15061 gcagcttcac gagaggcgac cgtaggccag gcgcaacacc agcactgcca gttcgcagag
    15121 ggtgctcagg agtcgatgtc gtagaggtcg cgcagggcct ggaccaggcg gtcgcccttg
    15181 gtggtgccgg ctgcgtcgcg cagggccacg gtgggctggt gcagcagctt ggccacgatg
    15241 ccctgggtga gcttctcgac cgcggcgatc tgggcgtcgt cgagctcggt gaggcgggcc
    15301 cgggcccgct ccagctcggc ggcgcgcagc gcctcggccc cctcgcgcag ggcgacgatg
    15361 accggggcga tctcccgggc ggacgtggcg gagcggtagc gctcgagctc ctcgtcgagg
    15421 atggcgcgga cggcgtcgac ctcccggcgg cgctcggcca ggccccggtc agcgaaggca
    15481 ccgaggtcgt ccatgtcgag gacggtgacg ccggcgagct cggccaccga gggctcgatg
    15541 tcgcggggca cggcgatgtc gacgagcacg aggggctggc cgttgcgggc ggccatcgcc
    15601 acctcgacgt cgtcccgggt gagcagcacc gaggcggcac cggtggaggt gagcacgacg
    15661 tcggcgtcga ccagctcgtc ggtgaggtcg gcgaagcgga tggcccgacc accgacggcg
    15721 gcggcgaggg ccttggcctt gtcgtgggtg cggttggcca cgatcagctc ggccacgccg
    15781 gcggaggcca gggactcggc catggacgaa cccatctcgc cggcgccgag cacgatggcc
    15841 cggcggccgt cgaggttgcc gagctgctgg gcggcgaggg ccacggcggc gtaggacacc
    15901 gaggcggtgc cctggccgat gccggtctcg ctgcgggccc gcttgccggc ctcgagggcg
    15961 tggcggaaga gcaggttcag ggtggtgccg gcggcgccct cgcgctgggc cttctcccac
    16021 gagatcttca cctggccctg gatctcgtgc tcgccgagga cggcggagtc gagcccggag
    16081 accacgtcga agaggtgggc gacggcggcg gtgtcgtggt gcacgtagag gtggtcggcg
    16141 aagtcctcgg cgtcgaggaa cgccagctcg gccagcacgg cgcggacgtc ggcgtagccg
    16201 ccgtggaact tctcggcgac cgcgtagacc tcggtgcggt tgcacgtgga gagcacgacg
    16261 gcctcggaca cgtgctcctt ggtgagcagc gcgtgcagcg tcttgccgag cgcagcgtcg
    16321 ccgacggtca tcgcctcgag caccccgagg ggggccgtgc ggtggttcac cccgatgacg
    16381 acgacaggca tgatctca
//
LOCUS       DATISU010000429         8165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_737520, whole genome shotgun sequence.
ACCESSION   DATISU010000429 DATISU010000000
VERSION     DATISU010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737520"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1865)
                     /locus_tag="VLR27_17095"
     CDS             complement(60..1865)
                     /locus_tag="VLR27_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05225.1"
                     /translation="MNRRLPVLVLLVAFVVAGVVLDREAADDATVEAVPVVPSDFPMA
                     PSPSALASTWFCAGGTADEEAFANHVVTMLNTTEGPLEVDVTAFPGIVAAPAPGAEAD
                     ALDPEAEPEEEEDAAAEDEEAEDAEEGPATDPASVSAEPVTERVEIEPRSRVRLALTD
                     LVTAPIASALVESDGGGLVVEHEVSSIHGVDAKPCATGASDEWHFAWGTTAREARELL
                     VLFNPFPDDAIVEGVFSTEDTVREPGRFAGGLVVPGRSTIGIDLGDDVTRRDEVAATL
                     TTRTGRLVVDRIVRVSEEGGDRGLTVQTGVNEPQRTWVFPDGLVSEEIGERFVVYNPS
                     EELAEVEIELRLDRPEENGIPAPVELSIAPGSHATVDMNEDGRLPPDVPHAAVVRSAN
                     DVPVVAEQVLSARRETGRRGISVTTGSPVEATEWTFAAGSTEEPSAESLAIVNLDPQV
                     LTEVDVFAVVGGRELPVADLQGVVIEAGERLHVNLTEQIANRGDLAVVVRATEPVVAA
                     RTLVLLGEDDRGVSLAVGVPSPDGIRVPADPVDLGLDADLGEDLEEAPTEDDEGIPVA
                     PDDVELPSPDETIVVDPDDESDVPAEGSDTTDGDG"
     gene            complement(1862..5164)
                     /locus_tag="VLR27_17100"
     CDS             complement(1862..5164)
                     /locus_tag="VLR27_17100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSP05226.1"
                     /translation="METPAPSRPAAPAVVAVVVTRDPGPWLEQAVDALAAQDYSNLAV
                     LFIDAASGTDPTPRIARALPEAYVRRLDENRGFGASANHVREIVDGASFYCFLHDDAA
                     PEPSAIRTMVDEAVRSNAGIVGGKLVQLDDRRRLLQVGESVDKTGERSTVVDPGELDQ
                     EQYDAVRDVFVVPGACTLVRADLFEAIGGFDEGIDLLNDDLSLCWRAHVAGARVVVAP
                     DAVIGHAEALGERSDTSLRRQRLVRHRLRTMLVCYSRWHRVRVLPQAALVAALEVVYS
                     LLTGRRSQARDVVAAWRWNWRRRDEIRALHRRVEDFRGVPDAEIRRFQVRGFSRLNAF
                     LRGTSSRGESPSDRAEGAWRTLVGGLRSGELRWPVIAWVITIVIVAFGSRHLIQRPIA
                     TVGGFLPFDEGPGELFDAFISGWRTTGLGSSSPAPTAFGLLAGAGVVFLGAMGTLRQV
                     LLLGAIVAGLVGAYRLLRPAASPRAQAVALVVYGAIPVAYDAVADARWGSLAVYAATP
                     WLLGQLGRAARWAPFGPVDGDTGPGVRAPDLLGRVASIGLLVALVAAIDPAVLPVVAV
                     VVVSLALGSVVAGAARGVPRLLVGGLGGVAVAVVLHLPWMLDFVLPGARWEQVVGHAG
                     VAETPTLGMLFRFDVGPVGGSVLALGFPIAATLPLLIGRGWRFDWAVRAWTVALVLIG
                     LTWAGGDDRLPLALPPAETMLAPAAASMAIAVALGVVAFEIDLRQYHFGWRQVASIVA
                     GVALVVGAIPTMLDAGNGRWYLPGGDLESTFGFLEEEDPGFRVLWIGHPDVLPLPGHS
                     LDLPDRDEPTSDHVVYATSQDGLPGITDGWPGSEDGPTQLIEDALVLAADGETSRLGR
                     LLAPMAIRYVVVPTASAPRPLGGVQRPAPAQVTGTLADQLDLAAVPVNPAYTVYRNEA
                     ALTSPAMIPPGAGSGTAFEVLAGDLEGAEPVLEERVGPTTWTGEVSAGARIHHASAAS
                     AGWELSVEGRSSPETKLFGWAQGFEPATSGDATLRYDTSPVRYGVLAFQAIFWLVAIH
                     FARRSRRQPPVVPAISAPPSTPEPVAPTQPADEPADWLAPAEEWPADPTPTLLDELEP
                     EPEPEPDEEERP"
     gene            5286..5624
                     /locus_tag="VLR27_17105"
     CDS             5286..5624
                     /locus_tag="VLR27_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05227.1"
                     /translation="MTPEITLDDIQKTVRDSFYVTVGLGVIAFQKAQVQRNELRKQLE
                     AGVGDAHQTVSDSVKTVEERLEAVEHRIDAVLDDVEEKLPAQARLVLSQARGVAKDAR
                     QQLRVLVDRA"
     gene            5818..6948
                     /locus_tag="VLR27_17110"
     CDS             5818..6948
                     /locus_tag="VLR27_17110"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HSP05228.1"
                     /translation="MPLRVAVDATPLLGVRTGVGVFTDALLRGLAGRDDIDVTAFPVS
                     VSGRSRFREVAPPGVRVRTPPLPARALHARWQRRSTPRIDHLVGRPDVVHGPNFVVPP
                     ARAARVATVHDLTTVRFPELCHADTLRFPDIVRRAAAEGAWIHTVSEAVRDEVVAELG
                     LDPERVVAVPNGFDAPVGDPAAGRRLAGVDRYVLAVGTVEPRKDLPSLVAAVDRLATE
                     HPDLVLIHAGGDGWQVERLEEALAAMAHPTRVRRLGHVPHDQLGHLYAGARVFAYPSV
                     YEGFGIPVLEAMACDVPVVTTAVPALREVAGDAAAIVPVGDVDALAGAIATAWSDETW
                     RAQAVERGRQRCERYSWEACVDGIVGIYRTAAAGSGRATTHT"
     gene            6959..7906
                     /locus_tag="VLR27_17115"
     CDS             6959..7906
                     /locus_tag="VLR27_17115"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HSP05229.1"
                     /translation="MRALVTGSLGFAGTHLCAHLRESGDDVIGIDRDDGDLTHPETAA
                     RLIGAHRPEVVYHLAGAANVGESWRDPVGTWDANATATLYLLEAARTHDVRRVLLVSS
                     ADVYGTVTEDELPLDESSPVRPTSPYAASKLAAEQVARQAWLGHGLETIQVRAFNHVG
                     PGQSPGFVAPALATAIARNELTGDTEIPIGNLSPRRDFTDVRDVARAYRLLMDQAEAG
                     ELYCVCSGRDIAIQELADTLVGMATSPMELVTDPDRYRPVDIPVLRGSNAKIHAATGW
                     SPSFSIADTLGDLLDECRARARSDVATHGNPKSTPRSSA"
     gene            7903..>8165
                     /locus_tag="VLR27_17120"
     CDS             7903..>8165
                     /locus_tag="VLR27_17120"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HSP05230.1"
                     /translation="MSTNPKALITGITGQDGSYLAELLLEKGYDVIGVARRSSTVTFE
                     RIAHLQEDIEIVFGDLLDQYSMIKLLEDHQPAEVYNLAAQSFVP"
BASE COUNT         1118 a         3074 c         2986 g          987 t
ORIGIN      
        1 caccagcgcg atcgccaccg cgacgacggc gacgaccgcg acgatgagga ggcgctccat
       61 cagccgtccc cgtcggtggt gtcggacccc tcggcgggga cgtcggattc gtcgtcgggg
      121 tcgacgacga tggtctcgtc gggcgacggg agctccacgt cgtcgggggc caccgggatc
      181 ccctcgtcgt cctcggtggg cgcctcctcg agatcctcgc ccaggtcggc gtcgaggccg
      241 aggtcgaccg ggtcggccgg gacgcggatc ccgtccgggg agggcacgcc cacggccagg
      301 gagaccccgc gatcgtcctc gcccagcagg accagcgtgc gagcggcgac cacgggctcg
      361 gtggcccgca cgaccacggc gaggtccccc cggttggcga tctgctccgt gaggttcacg
      421 tgcaggcgct cgccggcttc gatcacgacg ccctgcaggt cggccacggg cagctcccgg
      481 ccaccgacca cggcgaagac gtcgacctcg gtgagcacct gggggtcgag gttcacgatg
      541 gcgagggact cggcgctggg ctcctcggtg ctgccggcgg cgaaggtcca ctccgtggcc
      601 tcgaccggcg agccggtggt caccgagatg ccccggcgcc cggtctcccg ccgcgccgac
      661 agcacctgct cggccacgac cgggacgtcg ttcgcggagc ggaccacggc tgcgtgcggg
      721 acgtccggcg gcaaccggcc gtcctcgttc atgtccacgg tggcgtggga tccgggagcg
      781 atgctgagct cgacgggcgc agggatgccg ttctcctcgg gacggtcgag ccgcagctcg
      841 atctcgacct cggccagctc ctcgctcggg ttgtagacga cgaaccgctc accgatctcc
      901 tcggagacga ggccgtccgg gaagacccag gtgcgctgcg gttcgttcac gccggtctgc
      961 acggtgaggc cgcggtcgcc gccctcctcg ctgacccgca cgatgcggtc gaccaccagc
     1021 cggcccgtgc gggtcgtcag cgtcgccgcc acctcgtcgc ggcgggtcac gtcgtcaccg
     1081 aggtcgatgc cgatggtgct ccggcccggg accaccagcc cgccggcgaa gcgcccgggc
     1141 tcgcgcacgg tgtcctcggt ggagaagacc ccctcgacga tggcgtcgtc ggggaacggg
     1201 ttgaacagca cgagcagctc gcgggcctcg cgggccgtgg tgccccaggc gaagtgccac
     1261 tcgtcgctgg cgcccgtggc gcagggcttg gcgtcgacgc cgtggatgct ggagacctcg
     1321 tgctcgacca cgagaccgcc gccgtcggac tccaccaggg ccgatgcgat cggtgcggtg
     1381 accaggtcgg tgagggcgag gcgcacccgg ctccgcggtt cgatctcgac gcgctcggtc
     1441 acgggttcgg cgctcaccga cgccggatcg gtggccggcc cctcctcggc atcctcggcc
     1501 tcctcgtcct ccgctgccgc gtcttcctcc tcctcgggct cggcctcggg gtcgagggcg
     1561 tcggcctccg cgcccggggc cggggcggcc acgatgccgg ggaaggcggt gacgtcgacc
     1621 tcgaggggac cctcggtggt gttcagcatg gtgacgacgt ggttggcgaa ggcctcctcg
     1681 tcggccgtcc caccggcgca gaaccacgtg gaggcgagcg ctgagggtga gggcgccatc
     1741 gggaagtcgg aggggacgac cggcaccgcc tccaccgtgg cgtcgtcggc cgcctcgcgg
     1801 tcgagcacga cgccggccac gacgaaggcg acgagcagaa cgaggaccgg cagtcgacgg
     1861 ttcacggtcg ttcctcctcg tcgggttcgg gctccggctc cggctcgagc tcgtcgagca
     1921 gggtcggcgt gggatcggcc ggccactcct cggccggtgc caaccagtct gcgggctcat
     1981 cggccggttg ggtgggcgcg acgggctccg gggtgctcgg tggcgccgag atcgccggca
     2041 cgaccggggg ttggcgccgg gagcggcggg cgaagtggat ggcgacgagc cagaagatcg
     2101 cctggaacgc gagcacgccg tagcgcaccg gcgaggtgtc gtatcgcagg gtcgcgtcgc
     2161 cgctggtggc gggctcgaac ccctgcgccc agccgaacag cttggtctcg ggcgacgagc
     2221 gcccctcgac gctcagctcc cacccggccg acgcggccga cgcgtggtgg atgcgggcac
     2281 cggccgacac ctcgccggtc caggtggtgg gaccgacccg ctcctcgagc acgggctcgg
     2341 ctccttcgag gtcgccggcc aggacctcga aggcggtgcc ggaacctgca ccgggcggga
     2401 tcatcgccgg tgacgtcagc gccgcttcgt tgcggtacac ggtgtaggcg gggttgaccg
     2461 gcacggccgc gaggtcgagc tggtcggcca gggtcccggt gacctgcgca ggcgccggtc
     2521 gctgcacgcc gccgagggga cggggggccg acgcggtcgg caccaccacg tagcggatgg
     2581 ccatgggggc gagcagccgg cccagccggc tcgtctcccc gtcggccgcc agcacgagcg
     2641 cgtcctcgat cagctgggtc gggccgtcct cggagcccgg ccacccatcg gtgatgccgg
     2701 gcaggccgtc ctggctcgtg gcgtagacga cgtggtcgga cgtgggctcg tcccggtcgg
     2761 gcaggtcgag ggagtggccg gggaggggca gcacgtcggg gtggccgatc cacagcaccc
     2821 ggaagccggg gtcctcctcc tcgaggaacc cgaacgtgct ctcgaggtcg ccgcccggga
     2881 ggtaccagcg accgttgccg gcatcgagca tggtggggat ggcgcccacg accagggcga
     2941 cgcccgccac gatcgacgcg acctggcgcc agccgaagtg gtactggcgc aggtcgatct
     3001 cgaaggccac cacgccgagg gcgaccgcga tggccatcga cgccgcggcc ggggccagca
     3061 tcgtctccgc cggcggcagg gcgaggggca ggcggtcgtc gcctccggcc caggtcaggc
     3121 cgatcagcac cagcgcgacg gtccaggccc ggacggccca gtcgaagcgc cagccgcgac
     3181 cgatcagcag cgggagggtg gcggcgatcg ggaacccgag ggcgagcacg ctgccgccga
     3241 ccggtccgac gtcgaagcgg aagagcatgc cgagcgtcgg ggtctcggcg acgccggcat
     3301 ggccgaccac ctgctcccag cgggcgccgg ggagcacgaa gtcgagcatc cacggcaggt
     3361 gcagcaccac ggccaccgcc acgccgccga ggccaccgac cagcaggcgg ggcacgccac
     3421 gcgccgcgcc ggcgacgacc gaaccgagtg ccagggacac cacgacgacg gcgacgaccg
     3481 gcaggaccgc cggatcgatg gcggcgacga gcgccacgag gagcccgatc gaggccaccc
     3541 ggccgagcag gtcgggggcg cggaccccgg gaccggtgtc cccgtcgacg gggccgaacg
     3601 gcgcccaccg ggccgcacga ccgagctggc cgagcagcca cggcgtggcc gcgtagaccg
     3661 ccagcgaccc ccagcgggcg tcggcgacgg cgtcgtaggc gacggggatg gcgccgtaca
     3721 cgacgagggc cacggcctgc gcccgcggcg acgccgcggg gcggagcagg cggtaggcgc
     3781 ccaccaggcc ggccacgatg gcgccgagca ggagcacctg acgcaacgtg cccatggcac
     3841 cgaggaagac gaccccggcg ccggccagga ggccgaacgc ggtgggtgcc ggggatgacg
     3901 agccgagccc cgtggtgcgc cagccggaga tgaaggcgtc gaacagctcg ccggggccct
     3961 cgtcgaaggg caggaagccg cccacggtgg cgatggggcg ctggatcagg tgccggctgc
     4021 cgaacgccac gatcacgatc gtgatcaccc aggcgatcac gggccaccgc agctcgcccg
     4081 agcgcagccc gccgacaagg gtgcgccacg cgccctcggc ccggtccgac gggctctcgc
     4141 cgcgcgacga cgtgccccgc aggaaggcgt tgagccggga gaacccgcgg acctggaagc
     4201 ggcggatctc ggcgtcgggg acgccgcgga agtcctcgac ccggcggtgc agcgcccgga
     4261 tctcgtcgcg tcgtcgccag ttccaccgcc aggcggcgac gacgtcgcgc gcctggctgc
     4321 gccggccggt gagcagggag tacacgacct cgagcgcggc gacgagcgcg gcctggggca
     4381 gaacccggac ccggtgccag cggctgtagc agacgagcat cgtgcgcagc cggtggcgga
     4441 cgaggcgctg gcggcgcagg gaggtgtcgg agcgctcgcc cagcgcctcg gcgtggccga
     4501 tcacggcgtc gggggcgacc acgacccggg cgccggcgac gtgggcccgc cagcacaggc
     4561 tgaggtcgtc gttgagcagg tcgatgccct cgtcgaagcc gccgatggcc tcgaacaggt
     4621 cggctcgcac gagggtgcag gcgccgggga cgacgaacac gtcgcgcacg gcgtcgtact
     4681 gctcctggtc gagctcgccg gggtcgacca ccgtggagcg ctcgccggtc ttgtcgaccg
     4741 actcccccac ctgcagcagc cgccggcggt cgtcgagctg caccagcttg ccgccgacga
     4801 tgccggcgtt ggaccggacg gcctcgtcga ccatcgtgcg gatggcgctg ggctccgggg
     4861 cggcatcgtc gtggaggaag cagtagaagg acgcgccgtc gacgatctcg cgcacgtggt
     4921 tcgccgaggc gccgaagcct cggttctcgt ccaggcggcg cacgtacgcc tccggcaggg
     4981 cgcgcgcgat gcggggtgtc gggtcggtgc cgctcgccgc gtcgatgaac agcaccgcca
     5041 ggttggagta gtcctgggcg gcgagtgcgt ccacggcctg ctcgagccac ggcccgggat
     5101 cccgggtcac cacgacggcg acgaccgcag gggccgcggg gcggctcggg gcgggcgtct
     5161 ccacgagtgc ggcagggtac cgggccgagt cgcccggatc gcgaaggtcg gccggttcga
     5221 ccacgcgggg agacatcgtt cacaagtgct tgcaacgcct cgcacgcctg cttagtcttt
     5281 caagcatgac ccccgagatc accctcgacg acatccagaa gaccgtccgc gacagcttct
     5341 acgtgaccgt cggccttggc gtgatcgcgt tccagaaggc ccaggtccag cgcaacgagc
     5401 tgcgcaagca gctcgaggcc ggcgtgggcg acgcccacca gacggtctcc gacagcgtca
     5461 agaccgtcga ggagcgcctc gaggcggtcg agcaccgcat cgacgccgtc ctcgacgacg
     5521 tggaggagaa gctccccgcc caggcccgcc tcgtgctgtc ccaggcccgc ggcgtcgcca
     5581 aggacgcccg ccagcagctc cgggtcctcg tcgaccgggc ctgaccccat tcctccgctc
     5641 gacccgcttc ccccggcagg tcgagcggct gcgacggccg gcccctgacg gcagctccgc
     5701 tgcccaggcc ccggtcgtcg cccacctccg acgccgggca ccggcgcatc cgtcccccct
     5761 tggatgcgca cgcccggcgc ggaggtgccg atctccccgc ccgtaggctc gcccgccgtg
     5821 cccctccgag tcgccgtcga cgccacaccg ctgctcggcg tgcgcaccgg ggtcggggtg
     5881 ttcaccgatg cgctgctccg cgggctcgcc ggccgggacg acatcgacgt caccgcgttc
     5941 cccgtgagcg tgtcggggcg cagccgcttc cgggaggtcg cgccgccggg cgtgcgggtg
     6001 cgcaccccgc cgctgccggc tcgggcgctg cacgcccgct ggcagcggcg ctcgacgccg
     6061 cgcatcgacc acctggtggg acggcccgac gtggtgcacg gccccaactt cgtggtcccc
     6121 ccggcccggg cggcgcgcgt ggccaccgtg cacgacctca ccaccgtccg gttccccgag
     6181 ctgtgccacg ccgacaccct ccgcttcccc gacatcgtgc gacgggctgc cgccgagggg
     6241 gcctggatcc acaccgtgtc cgaggccgtg cgggacgagg tggtcgccga gctcggcctc
     6301 gaccccgagc gggtcgtcgc cgtccccaac ggcttcgacg cgccggtggg cgatccggcc
     6361 gccgggcgcc gcctcgccgg ggtcgaccgg tacgtgctgg ccgtcggcac cgtggagccc
     6421 cgcaaggacc tgccctcgct ggtcgcagcc gtcgaccggc tcgccaccga gcacccggac
     6481 ctcgtcctga tccacgccgg cggcgacggc tggcaggtgg agcggctcga ggaggccctg
     6541 gccgcgatgg cgcacccgac ccgcgtccgc cggctcggcc acgtccccca cgaccagctc
     6601 ggccacctct acgccggcgc acgggtcttc gcgtacccgt cggtgtacga gggcttcggc
     6661 atcccggtgc tcgaggcgat ggcgtgcgac gtgcccgtgg tgaccaccgc cgtgcccgcc
     6721 ctccgcgagg tcgccggcga cgcggccgcg atcgtcccgg tgggcgacgt cgacgccctg
     6781 gccggcgcca tcgccaccgc ctggtcggac gagacgtggc gggcacaggc cgtcgagcgg
     6841 ggccgccagc ggtgcgagcg gtactcctgg gaggcgtgcg tggacggcat cgtcggcatc
     6901 taccggacgg cggccgcagg ttccggccgg gccacgactc acacgtagac tcgcccggat
     6961 gcgtgccctg gtcaccggat cgctcggctt cgccggcacc cacctgtgcg cgcacctgcg
     7021 cgagtccggc gacgacgtca tcggcatcga ccgcgacgac ggcgacctca cgcaccccga
     7081 gaccgccgcc cgcctcatcg gcgcccaccg gcccgaggtc gtctaccacc tcgccggggc
     7141 ggccaacgtc ggcgagtcct ggcgggaccc ggtcggcacc tgggacgcca acgccaccgc
     7201 cacgctctac ctgctcgagg ccgcccgcac ccacgacgtc cggcgcgtgc tgctcgtgtc
     7261 cagcgccgac gtctacggca ccgtcaccga ggacgagctg cccctcgacg agagctcgcc
     7321 cgtgcggccc accagcccgt acgccgcctc gaagctggcg gccgagcagg tggcccgtca
     7381 ggcctggctg ggccacggcc tcgagacgat ccaggtgcgg gcgttcaacc acgtcgggcc
     7441 cggccagtcc cccggcttcg tggcccccgc cctggccacc gccatcgccc gcaacgagct
     7501 gaccggcgac accgagatcc ccatcggcaa cctgagcccc cggcgcgact tcaccgacgt
     7561 ccgcgacgtg gcccgcgcct accggctcct catggaccag gccgaggccg gcgagctgta
     7621 ctgcgtctgc tcgggccgcg acatcgccat ccaggagctg gccgacaccc tcgtcggcat
     7681 ggccaccagc ccgatggagc tggtcaccga ccccgaccgg taccgcccgg tcgacatccc
     7741 cgtcctgcgg ggatccaacg ccaagatcca cgccgccacc ggctggtccc cgtcgttctc
     7801 gatcgccgac accctcggcg acctgctcga cgagtgccgg gcccgggcgc ggtccgacgt
     7861 cgccacccac ggcaacccca agagcacccc acggagcagc gcatgagcac caaccccaag
     7921 gccctcatca ccggcatcac cggccaggac ggctcgtacc tggccgagct gctgctcgag
     7981 aagggctacg acgtcatcgg cgtcgcccgc cggtccagca ccgtcacctt cgagcggatc
     8041 gcccacctcc aggaggacat cgagatcgtc ttcggcgacc tgctcgacca gtactccatg
     8101 atcaagctgc tcgaggacca ccagccggcc gaggtctaca acctggccgc ccagtccttc
     8161 gtgcc
//
LOCUS       DATISU010000430         4210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_412257, whole genome shotgun sequence.
ACCESSION   DATISU010000430 DATISU010000000
VERSION     DATISU010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412257"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VLR27_17125"
     CDS             <1..243
                     /locus_tag="VLR27_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05231.1"
                     /translation="RADQQARLNEELAAARTQAQADIDAAKGQAMSELRGEVATIAIG
                     AAESVVGANLDRDAQTRLIEDYINRVQAAPSGNGQG"
     gene            246..773
                     /gene="atpH"
                     /locus_tag="VLR27_17130"
     CDS             246..773
                     /gene="atpH"
                     /locus_tag="VLR27_17130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HSP05232.1"
                     /translation="MGTERDKAYAMAIAAVGRAEGELDEVQDELFSLSRTLGGSEELL
                     STLTDAQVPVARRQQIVEDLLAGKVSKSTIATVSMVVAAGRARDLTGIADELVALGAA
                     EGGRDVAVVRSAIDLTDDQKARLATALQQAVGRPVDIRVVIDPSVLGGLVAQVGDTVI
                     DGSVRRRLDLLKQAI"
     gene            787..2319
                     /gene="atpA"
                     /locus_tag="VLR27_17135"
     CDS             787..2319
                     /gene="atpA"
                     /locus_tag="VLR27_17135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HSP05233.1"
                     /translation="MTDLNINSGDIAAAIRKNLEGFDPSIGTATVGRIAEVGDGIARV
                     TGLPGCAVNELLEFEDGTKGLALNLDEESIGAVVLGEVDHINEGQAVRATGEILSVPC
                     GDGLLGRVVDALGNPVDGKGPLQNVDTRRMEIQAPGIMGRQPVHEPLQTGIKAIDSMT
                     PIGRGQRELIIGDRKTGKTVVAIDTILNQKGLGVKCIYVAIGQKESTVAQTVSTLAEH
                     GAMDYTVVVVAGGSDPAPFKYLAPYAGCAMGQHWMENSQHALVVYDDLSKQAEAYRQV
                     SLLLRRPPGREAYPGDVFYLHSRLLERAAKLSDELGAGSLTALPIIETKAGDISAYIP
                     TNVISITDGQVFLQEDLFKSGIRPAFDTGQSVSRVGGAAQIKAMKTAVGTLKGDLSQF
                     RELESFAQFGSELDAVSQATLDRGYRLVELLKQDLNSPLRVEEQVIVLYAGTQGFLDS
                     IPVEDVKRYEADLLAWFEARHRDILDGIRETGKIPSEDDLKAALQGFTDQFETTVSTE
                     ES"
     gene            2319..3287
                     /locus_tag="VLR27_17140"
     CDS             2319..3287
                     /locus_tag="VLR27_17140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004145.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HSP05234.1"
                     /translation="MAGGKERVLRRRIKSVQTTKKITKAMELIAASRIVKSQQRVLAA
                     RPYADQITEVIRNLAAAGAGGGAPLLAKREQVQTIATIVVGGDRGLSGAYNSNAIRMG
                     ERNIAAQRREGKDTVLIAVGKKVESYFRFRKYDIADSFTGFLDEPHYEDARRIAEAVV
                     PKFEDGTYDRVDIVYTQFLSAGTQQVVTRQFLPLDTSAITETATDGPSADYEFEPGPE
                     QILDRLLPRYAEARLFSALLEGAASFFAAQQRAMKSATDNAEELITKLSREMNRARQD
                     SITTEIMEIVGGAEALRQSKSEGMDLIPDDALSIRDLLVDTVDYNR"
     gene            3297..>4210
                     /locus_tag="VLR27_17145"
     CDS             3297..>4210
                     /locus_tag="VLR27_17145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222616.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HSP05235.1"
                     /translation="MTVTEPTSQEAGQLKSGRIVAIAGPVVDVEFPTGELPDINFALE
                     MTINVGGEDITVVAETAQQLGNSRVRAICMKPTDGLVRGTEVRNLGHPLQMPVGDGVL
                     GHVLNVLGEPLDVESLDMSKIDGYRPIHRAAPDFADLAPKKEVFETGIKVIDLLTPYL
                     RGGKIGLFGGAGVGKTVLITEMINRVASQFGGVSAFAGVGERTREGTDLFIEMGETMI
                     DENTSVLDKAALVFGQMDEPPGVRLRVALSALTIAEYFRDERNQDVLLFVDNIFRFVQ
                     AGSEVSTLLGRMPSAVGYQPTLADEMGTL"
BASE COUNT          714 a         1585 c         1327 g          584 t
ORIGIN      
        1 cgcgccgacc agcaggcccg cctgaacgag gagctcgccg ctgctcgcac ccaggcccag
       61 gccgacatcg acgccgccaa gggccaggcc atgtccgagc tccgcggcga ggtcgccacc
      121 atcgccatcg gcgcagccga gagcgtcgtg ggcgccaacc tcgaccgcga cgcccagacc
      181 cgcctcatcg aggactacat caaccgggtc caggccgctc cctccggcaa cgggcagggc
      241 tgatcatggg caccgagcgt gacaaggcat acgccatggc gatcgccgcg gtggggcgcg
      301 ccgagggcga gctcgacgag gtgcaggacg agctgttcag cctgtcccgc acgctcggcg
      361 gctccgagga gctgctgtcg acgctgaccg acgcccaggt gccggtcgcc cgtcgacagc
      421 agatcgtcga ggacctcctc gccgggaagg tgtccaagtc caccatcgcc accgtgtcga
      481 tggtcgtggc cgccggccgc gcccgcgacc tcaccggcat cgccgacgag ctcgtcgcgc
      541 tcggtgccgc cgaaggtggc cgcgacgtcg cggtggtgcg gtcggccatc gacctcaccg
      601 acgaccagaa ggcccgcctg gccactgcgc tgcagcaggc cgtgggccga ccggtcgaca
      661 tccgcgtcgt catcgatccc tccgtgctcg gagggctggt cgcccaggtc ggcgacaccg
      721 tcatcgacgg ctccgtccgt cggcgtctcg acctgctgaa gcaagccatc taggggctca
      781 catccaatga ctgatctgaa catcaactcc ggcgacatcg ccgccgccat ccgcaagaac
      841 ctcgaggggt tcgacccgtc gatcggcacg gccaccgtcg gccgcatcgc cgaggtcggc
      901 gacggcatcg ctcgcgtcac cggcctgccc ggctgcgccg tgaacgagct gctcgagttc
      961 gaagacggca ccaagggcct ggccctgaac ctcgacgagg agtcgatcgg tgccgtggtg
     1021 ctcggtgagg tcgaccacat caacgagggc caagcggtcc gcgccaccgg cgagatcctg
     1081 tcggtgccct gcggcgacgg cctcctcggc cgcgtcgtcg acgccctcgg caaccccgtc
     1141 gacggcaagg gcccgctcca gaacgtcgac acccgccgca tggagatcca ggcccccggc
     1201 atcatgggcc gccagcccgt gcacgagccg ctgcagaccg gcatcaaggc catcgactcg
     1261 atgaccccga tcggtcgtgg ccagcgagag ctgatcatcg gcgaccgcaa gaccggcaag
     1321 accgtcgtgg cgatcgacac catcctgaac cagaagggtc tcggggtgaa gtgcatctac
     1381 gtcgccatcg gtcagaagga gtccacggtc gcccagaccg tgagcaccct cgccgagcac
     1441 ggcgccatgg actacacggt cgtggtcgtg gccggcggct ccgatccggc gccgttcaag
     1501 tacctggccc cctacgccgg gtgcgccatg ggccagcact ggatggagaa cagccagcac
     1561 gccctcgtcg tgtacgacga cctgtccaag caggccgagg cgtaccgcca ggtgtcgctg
     1621 ctgctgcgcc gcccgccggg ccgcgaggcc tacccgggcg acgtgttcta cctgcacagc
     1681 cgcctgctcg agcgcgccgc caagctctcc gacgagctcg gcgccggttc gctcaccgcc
     1741 ctgccgatca tcgagaccaa ggccggcgac atctcggcct acatcccgac caacgtgatc
     1801 tcgatcaccg acggccaggt gttcctccag gaggacctct tcaagtccgg catccgtccg
     1861 gcgttcgaca cgggtcagtc ggtgtcccgt gtgggtggcg ccgcgcagat caaggccatg
     1921 aagaccgcgg tgggcacgct caagggcgac ctgtcccagt tccgcgagct cgagtccttc
     1981 gcccagttcg gttccgagct cgacgccgtg tcccaggcca cgctcgaccg cggctaccgc
     2041 ctggtggagc tgctcaagca ggacctcaac tcgccgctgc gggtcgagga gcaggtgatc
     2101 gtgctctacg ccggcaccca gggcttcctc gacagcatcc cggtcgagga cgtgaagcgc
     2161 tacgaggccg acctcctggc ctggttcgag gctcgccacc gcgacatcct cgacggcatc
     2221 cgggagacgg ggaagatccc gtccgaggac gacctcaagg cagcactgca gggcttcacc
     2281 gaccagttcg agaccacggt cagcaccgag gagtcgtagt ggcaggtggc aaggagcgcg
     2341 tcctgcgccg gcgcatcaag tcggtgcaga ccacgaagaa gatcacgaag gcgatggagc
     2401 tcatcgccgc cagtcggatc gtcaagagcc agcaacgcgt cctcgccgcc cgcccgtacg
     2461 ccgaccagat caccgaggtg atccggaacc tcgcggcggc cggtgccggt ggcggcgccc
     2521 cgctgctcgc caagcgcgag caggtccaga ccatcgccac catcgtcgtc ggcggcgacc
     2581 gcggcctgtc gggcgcctac aactccaacg ccatccgcat gggcgagcgc aacatcgccg
     2641 cccagcgccg cgagggcaag gacacggtgc tgatcgccgt gggcaagaag gtcgagagct
     2701 acttccgctt ccggaagtac gacatcgccg actccttcac cggcttcctc gacgagcccc
     2761 actacgagga cgcccggcgc atcgcggagg ccgtcgtccc caagttcgag gacggcacct
     2821 acgaccgggt ggacatcgtc tacacccagt tcctgtcggc cggcacccag caggtggtca
     2881 cccgccagtt cctgccgctc gacacctcgg ccatcaccga gaccgccacc gacggtccct
     2941 ccgccgacta cgagttcgag cccggacccg agcagatcct cgaccggctg ctgcctcgct
     3001 acgccgaggc ccgcctcttc tccgccctcc tcgagggcgc ggcctccttc ttcgccgccc
     3061 agcagcgcgc catgaagtcg gccaccgaca acgcggagga gctcatcacc aagctctccc
     3121 gcgagatgaa ccgcgcccgc caggactcca tcacgaccga gatcatggag atcgtcggtg
     3181 gtgccgaggc cctccgccag agcaagagcg aaggcatgga cctgatcccc gatgatgccc
     3241 tcagcatccg agacctgctc gtcgacaccg tcgactacaa ccgctaggag caccccgtga
     3301 ccgtcaccga gcccaccagc caagaagccg gccagctcaa gtcgggccgc atcgtggcca
     3361 tcgccggccc cgtggtcgac gtggagttcc ccaccgggga gctccccgac atcaacttcg
     3421 cgctcgagat gaccatcaac gtcggcggcg aggacatcac cgtcgtcgcc gagaccgcgc
     3481 agcagctcgg caacagccgc gtgcgggcca tctgcatgaa gcccaccgac ggcctggtcc
     3541 gtggcaccga ggtgcgcaac ctcggccacc ccctccagat gccggtcggc gacggcgtgc
     3601 tcggccacgt gctgaacgtc ctcggcgagc ccctcgacgt cgagtccctc gacatgtcca
     3661 agatcgacgg ctaccgcccg atccaccgcg ccgcccccga cttcgccgac ctcgctccga
     3721 agaaggaggt gttcgagacc ggcatcaagg tcatcgacct cctgaccccg tacctgcgag
     3781 gcggcaagat cggcctgttc ggcggcgccg gcgtgggcaa gaccgtgctc atcaccgaga
     3841 tgatcaaccg cgtcgcctcc cagttcggtg gcgtctccgc cttcgccggg gtgggcgagc
     3901 gcacccgtga gggcaccgac ctgttcatcg agatgggcga gaccatgatc gacgagaaca
     3961 cgtcggtcct cgacaaggcc gccctcgtgt tcggccagat ggacgagccc ccgggcgtgc
     4021 gcctgcgcgt cgccctgtcg gccctcacca tcgccgagta cttccgcgac gagcgcaacc
     4081 aggacgtgct gctcttcgtc gacaacatct tccggttcgt gcaggccggc tccgaggtct
     4141 ccaccctcct cggccgcatg ccctccgccg tgggctacca gcccacgctg gccgacgaga
     4201 tgggcaccct
//
LOCUS       DATISU010000431         8249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_737861, whole genome shotgun sequence.
ACCESSION   DATISU010000431 DATISU010000000
VERSION     DATISU010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737861"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VLR27_17150"
     CDS             complement(<1..587)
                     /locus_tag="VLR27_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05236.1"
                     /translation="MERGTKYLLSSSMRALHAANARNPISRSGPLSIPSFAWGLPGSE
                     LPRPHMALHAASMAWAIARGSHRTLAGKAGLALSAASIATLWKVHQQSTDSEQLVERA
                     LRDQLGDDYRSRMSESPIEATRTKLPRIPTRSVRKRYVDGGHIQYSEYGKRTTLDVWK
                     RPDLPADAGAPVMVQVHGGAWVLGDKEGQAYPLMA"
     gene            complement(639..1646)
                     /gene="ligD"
                     /locus_tag="VLR27_17155"
     CDS             complement(639..1646)
                     /gene="ligD"
                     /locus_tag="VLR27_17155"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HSP05237.1"
                     /translation="MATSDAVELEISGHTVRITSPDKVFFPERGETKLDLVEYYLTVA
                     DPFLRAVGGRPTLLQRFPDGVDGSSFFQKRVPKGAPDWLRTTTMSTPNGTTSNAMVLE
                     DIAHVAWAVNMGCLGFHPWPCRADDPGTTDELRIDLDPSPGVDFPMLREAAQELRSLL
                     DEDGLASFPKTTGNRGIHVHVPIGPGWDSYDVRLGAVAVARELERRRPELITAAWWKE
                     ERGSRVFVDFNQNAPHKTVFGAWSVRARPGAQVSTPFRWDELDQIHPDVLTMETVPPR
                     VEEHGDPWAGIDGVTQSLEPLLTRSAADRQAGLPDAPWPPVYPKMPDEPPRVAPSRAA
                     R"
     gene            1679..2563
                     /locus_tag="VLR27_17160"
     CDS             1679..2563
                     /locus_tag="VLR27_17160"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HSP05238.1"
                     /translation="MTEVQVLGNPAARGGTGDVERVVDEIRAGGRRAALLRADSAEAS
                     AAAARAAVEAGHDRLIVVGGDGLVRIAVDAVAESGITLGIVPQGTGNDFARALGLLDG
                     DLGEHVTRALADPVPVDAMRTNHGWVASVATVGFSGDVTARANGLPWPRGQQRYTVAT
                     VLQLPRLRTIRARITVDGTTHDAATTMLSVGNTAYFGGGMRICPDATPSDGLLHAVVI
                     GDVGRGTFLRVFPRVFRGGHVKHPDVATYRGRTVVVEAADPDAAAVEMWADGDLLGPL
                     PVTCEVVPGALRVAGAHV"
     gene            complement(2613..2972)
                     /locus_tag="VLR27_17165"
     CDS             complement(2613..2972)
                     /locus_tag="VLR27_17165"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HSP05239.1"
                     /translation="MTALAVAAAGAVGALLRWRIGVAIGLRSFPWATLAVNVVGCFAL
                     AAVIAGPGASRWSPTTTTAVAVGLLGSFTTFSTFGYETFTLLRTDEPGRAAAYVALSL
                     SGGLAATALGWAVGRSL"
     gene            complement(2969..3730)
                     /locus_tag="VLR27_17170"
     CDS             complement(2969..3730)
                     /locus_tag="VLR27_17170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSP05240.1"
                     /translation="MTEQHSTTSDEFAGRVAVVTGSSSGIGEEIARRLAAGGASVVVN
                     SATSVEAGTAVAESLPTESIYVQADISDADQCSALVDATIERFGKLDYLINNAGWTTV
                     IPHHDLDALTDDVFRRTFDVNVFGTWSLTKAAMPHLKQSDDGNVVSITSLAGVRPVGS
                     SIAYAMTKAALNHMTVLLAKSHGPVRFNAVAPGLVDTPWTADWGPMHEAVAAMAPVPR
                     SATPEDCAEATLALIRNRYTTGHVFVVDGGLSLVG"
     gene            3796..4758
                     /locus_tag="VLR27_17175"
     CDS             3796..4758
                     /locus_tag="VLR27_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSP05241.1"
                     /translation="MDMEYRRLGRSGLKVSVLSFGSWVTFGPQLDSGLAADCMGAAFD
                     AGVNFFDNAEAYAGGESERLMGEAIRKNGWKRHEYVVSSKFYWGIHDGPNSRLTLNRK
                     YLLEAIPSSLERIGLDSYDLVFCHRADPDTPIEETVWAMSDAIDRGWAYYWGTSEWTA
                     DEIRAAYEIAQRHHLHAPVMEQPQYNLLARRKVEKEYARLYEDIGLGLTIWSPLASGL
                     LTGKYVDGIPEGSRAALEGYEWIKDRVTDEEVNTKVREFMGIADELDATPAQLALAWC
                     AKNPNVSTVITGASRVEQVTENMKAIEVLGRLDDATAERIASIF"
     gene            4764..5237
                     /locus_tag="VLR27_17180"
     CDS             4764..5237
                     /locus_tag="VLR27_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05242.1"
                     /translation="MGVDVEFLEFDPDDPGEVLAAMADLAGGAGWLTLDPAIDDRFPA
                     PEQNLFGKIVSGRGPAVPRATWVPADVERRRPEPMSVGILHGTGADAIGRLAEKDIVL
                     PDRWRVVADHSKRGLVAWVPIDIPLDQVLAWTLTASRALTRVPLTGRWRAAVHRR"
     gene            5248..6075
                     /locus_tag="VLR27_17185"
     CDS             5248..6075
                     /locus_tag="VLR27_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HSP05243.1"
                     /translation="MSDPTEQATFEQMMALEPHGADTYVGVGPRYPWGGLYGGQIVAQ
                     ALRAAGLTVEPEYLPHSLHAYFIRPGDHTEPIRFEVDRIRNGRSFVTRRVVARQSIGA
                     IFNLAASFHVVEDEADVQAATLSPDAGSPDDHLSDGWSHVFDRRPVGDMRRSDAGLSR
                     AWLRMVEPVADDPLLRACAISYVSDDLPTDGAIALHPDRKLVREEGDWERTFMNASLD
                     HAIWFHRTVDPAEWHLHELRGHNLTGARGLAIGEVFSQAGVHAATVVQEVLLRRVRS"
     gene            6080..7132
                     /gene="mraY"
                     /locus_tag="VLR27_17190"
     CDS             6080..7132
                     /gene="mraY"
                     /locus_tag="VLR27_17190"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HSP05244.1"
                     /translation="MIAVLAAALVALLIGLVGTSYLIGWFRRHGIGQPIHTAVTQHAA
                     KAGTPTMGGLSIVLAAPIGYGVGLAVLGRAPTRTGIVLVVAILAGGAVGVLDDWMKIS
                     RGRNTLGLQERQKTLLILAVGLLLFVTQSSDETSACTAPSLARCEPFADLPGPLWLLW
                     VLGVVWLTTNSVNFTDGLDGLLAGTSIAPLALLSLIAFWQFRHQPAYPASEGALDVAL
                     VMVAVTACCAGLLWWSAAPARVFMGETGSLAIGTAIAVAALLLHVDLLIPVFGALYVA
                     EGLSSFAQRVWFKATRRFRRDHEPQRLFRMAPLHHHFELVTPETTVVIRFWIVSAIAS
                     AGAGAIFYWDALRVLE"
     gene            complement(7190..>8249)
                     /locus_tag="VLR27_17195"
     CDS             complement(7190..>8249)
                     /locus_tag="VLR27_17195"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSP05245.1"
                     /translation="IAEAVGATRIGVLQMVGDFLDLDHEFGTLVEMMRASGRPLSFSL
                     AQSPLAPDQFRELLKRLDAAVDDGFDMRAQVAPRAVGLLLGLDCTLNPFLTNPVWREV
                     ADRPLAEQVATMSDPEFRRRVLEAHEAGKAKGKLGGGLIGAFDRMFPLDDPPEYEPDL
                     DDAVTGQAQRAGVGPVEWAYDFMLGDGGAAMFYVPFLNYAEGNLDTTREMLAHPRTVP
                     GLSDGGAHVGTICDGSFPTFLLSHWGRDRQRGELFDIEYLVRQHTQDTARTVGLLDRG
                     VLAPGMRADVNVIDFDRLAVSRPEMHHDLPAGGRRVLQRATGYRHTVVAGVETYADGE
                     ATGELPGRLVRGAQPAPR"
BASE COUNT         1104 a         2968 c         3012 g         1165 t
ORIGIN      
        1 tgggccatca gcgggtacgc ctggccctcc ttgtcaccga ggacccaggc gccgccgtgc
       61 acctgcacca tgaccggggc gccggcgtcg gcggggaggt cgggccgctt ccacacgtcg
      121 agcgtggtcc gcttgccgta ctcgctgtac tggatgtggc cgccgtcgac gtagcgcttg
      181 cggaccgagc gggtggggat ccggggcagc ttggtgcggg tggcctcgat gggcgactcc
      241 gacatgcggc tgcggtagtc gtcgccgagc tggtcccgca gggcccgctc gacgagctgc
      301 tcggagtcgg tcgactgctg gtgcaccttc cacagcgtgg cgatggacgc agcgctgagg
      361 gcgaggccgg ccttgccggc cagggtgcgg tgcgacccgc gggcgatggc ccaggccatc
      421 gacgccgcgt gcagcgccat gtgcggccgg ggcagctcgg agccgggcag cccccaggcg
      481 aaggacggga tgctgagcgg gcccgatcgg gagatcgggt tgcgggcgtt ggcggcgtgc
      541 agggcccgca tcgagctcga gagcaggtac ttcgtgcctc gttccatccg gtcaccctcg
      601 cacccggcgc tgcggggccg ccaccccgtg cccggtggtc agcgggcggc gcggctcggt
      661 gccacccggg gcggttcgtc gggcatcttc gggtagacgg gcggccaggg cgcgtccggc
      721 aacccggcct ggcggtcggc tgcggaccgg gtgagcaggg gctcgagcga ctgggtgacg
      781 ccgtcgatgc cagcccacgg gtcgccgtgc tcctcgaccc ggggcgggac ggtctccatg
      841 gtgaggacgt cggggtggat ctggtcgagc tcgtcccagc ggaacggcgt gctcacctgc
      901 gcgccgggcc gggcccggac cgaccaggcg ccgaacacgg tcttgtgggg tgcgttctgg
      961 ttgaagtcga cgaagacgcg actgccgcgc tcctccttcc accacgccgc ggtgatcagc
     1021 tccggccggc gtcgctccag ctcccgcgcc accgcaacgg cgccgaggcg cacgtcgtag
     1081 gagtcccagc ccggcccgat cggcacgtgc acgtggatgc cgcggttgcc ggtggtcttc
     1141 gggaacgacg ccagaccgtc ctcgtcgagc agcgagcgca gctcctgcgc cgcttcgcgg
     1201 agcatcggga agtccacgcc gggggagggg tcgaggtcga tgcggagctc gtcggtcgtg
     1261 cccgggtcgt cagcccggca cggccagggg tggaacccga ggcaacccat gttgacggcc
     1321 catgcgacgt gcgcgatgtc ctccagcacc atggcgttgg acgtggtgcc gttgggtgtg
     1381 ctcatggtcg tggtccgcag ccagtccggc gcccccttgg gcacccgctt ctggaagaac
     1441 gagctgccgt ccacgccgtc ggggaaccgc tgcagcagcg tcggccggcc gcccaccgcc
     1501 cgcaggaagg ggtcggccac ggtcaggtag tactcgacca ggtccagctt ggtctcgccc
     1561 cgctccggga agaacacctt gtccgggctg gtgatgcgca ccgtgtgccc gctgatctcg
     1621 agctcgacgg cgtcggaggt cgccatcgcc gggacggtag cggcctacgg tgcgagacgt
     1681 gaccgaggtg caggtgctgg ggaaccccgc ggcgcggggc gggacgggcg acgtggagcg
     1741 ggtggtcgac gagatccgtg cgggtgggcg acgagcggcc ctcctccggg cggactccgc
     1801 cgaggcatcg gccgcggctg ctcgcgccgc ggtggaggcc ggccacgacc gcctgatcgt
     1861 cgtcgggggc gacgggctcg tgcgcatcgc cgtcgacgcg gtcgccgagt cggggatcac
     1921 gctggggatc gtgccgcagg gaacgggcaa cgacttcgcc cgggccctcg gcctgctcga
     1981 cggtgacctc ggcgagcacg tcacgagggc gctggccgac ccggtgccgg tcgatgccat
     2041 gcgcacgaac cacggctggg tcgccagcgt ggccacggtc ggcttcagcg gcgacgtcac
     2101 cgcccgggcc aacggactgc cgtggccccg gggccagcag cgctacacgg tggccaccgt
     2161 gctgcagctg ccccggctgc gcaccatccg ggcccgcatc accgtcgacg gcacgacgca
     2221 cgacgccgcc accacgatgc tgtcggtcgg caacaccgcg tacttcggcg gcggcatgcg
     2281 gatctgcccc gacgccaccc cgtccgacgg gctcctccac gcggtcgtga tcggcgacgt
     2341 cgggcgggga acgttcctgc gggtgttccc gcgcgtcttc cggggcggcc acgtgaagca
     2401 ccccgacgtc gccacctacc ggggacgcac cgtggtggtc gaggccgccg atcccgacgc
     2461 cgccgccgtg gagatgtggg ccgacgggga cctgctcggc ccgctccccg tcacctgcga
     2521 ggtcgtgccc ggcgcgctcc gggtcgccgg cgcccacgtc tgatcggttc tgacggcggc
     2581 cacggaacgc gttccgctgt cgctgccaga tctcagaggc tccggcccac ggcccagccc
     2641 agtgcggtgg cggccagccc tccggacagc gacagcgcca cgtacgccgc ggcgcgaccc
     2701 ggctcgtcgg tgcgcaggag cgtgaaggtc tcgtagccga aggtcgagaa ggtggtgaac
     2761 gacccgagca ggcccacggc caccgcggtc gtcgtcgtgg gcgaccagcg cgacgcaccg
     2821 ggtccggcga tcaccgcggc gagcgcgaag cagccgacga cgttcacggc cagggtcgcc
     2881 cacgggaacg accgcagccc gatggcgacc ccgatgcgcc agcggaggag ggcgccgacg
     2941 gccccggcgg ccgcgacggc gagggcagtc aaccgacgag cgacaggccg ccgtcgacga
     3001 cgaagacgtg cccggtcgtg tagcggttgc ggatgagggc gagcgtggcc tcggcgcagt
     3061 cctcgggggt ggcgctgcgc ggcaccgggg ccatggcggc gacggcctcg tgcatcgggc
     3121 cccagtcggc ggtccacggc gtgtcgacca ggccgggggc gacggcgttg aaccgcaccg
     3181 gaccgtgcga cttcgccagc agcaccgtca tgtggttcag cgccgccttc gtcatggcgt
     3241 aggcgatgga ggacccgacc gggcgcacgc cggcgaggga cgtgatggac acgacgttgc
     3301 cgtcgtcgct ctgcttcagg tggggcatgg ccgccttggt gagcgaccac gtgccgaaca
     3361 cgttcacgtc gaaggtgcgg cggaacacgt cgtcggtgag ggcgtcgagg tcgtggtggg
     3421 ggatgaccgt ggtccacccg gcgttgttga tgaggtagtc gagcttcccg aaccgctcga
     3481 tggtggcatc caccagcgcg ctgcactggt cggcgtcgct gatgtcggcc tgcacgtaga
     3541 tcgactccgt cggcagcgac tcggccaccg cggtgccggc ctcgaccgac gtcgccgagt
     3601 tcaccacgac gctcgctccg cccgcggcca gccgtcgggc gatctcctcg ccgatgccgc
     3661 tcgaggagcc ggtcacgacg gcgacgcgcc cggcgaactc atcggaggtg gtgctgtgct
     3721 gctcggtcat ggggcgtgcc taccaccgac gggctcgcgg tgcggctccg gggcggcgct
     3781 cgtagggtgc gagccatgga catggaatat cgccgcctcg gacgttccgg gctcaaggtc
     3841 agcgtgctgt cgttcggcag ctgggtgacc ttcggccccc agctcgactc cggtctcgcc
     3901 gccgactgca tgggcgcggc cttcgacgcc ggcgtcaact tcttcgacaa cgccgaggcc
     3961 tacgccggcg gcgagtccga gcgcctgatg ggcgaggcca tccgcaagaa cgggtggaag
     4021 cgccacgagt acgtcgtgtc gtccaagttc tattggggca tccacgacgg tccgaacagc
     4081 cgcctgaccc tgaaccgcaa gtacctcctg gaggccatcc cctcgtcgct cgagcgcatc
     4141 ggcctcgaca gctacgacct cgtcttctgc caccgcgccg atccggacac gcccatcgag
     4201 gagacggtgt gggccatgtc cgacgccatc gaccgtggct gggcgtacta ctggggcacc
     4261 tcggagtgga cggccgacga gatccgcgcc gcctacgaga tcgcccagcg ccaccacctg
     4321 cacgcgccgg tgatggagca gccccagtac aacctgctcg cccgccgcaa ggtcgagaag
     4381 gagtacgccc gcctgtacga ggacatcggc ctcgggctca ccatctggtc gccgctggcc
     4441 tcgggcctgc tcaccggcaa gtacgtcgac ggcatccccg agggctcccg cgccgcgctc
     4501 gagggctacg agtggatcaa ggaccgcgtc accgacgaag aggtcaacac caaggtgcgc
     4561 gagttcatgg gcatcgccga cgagctcgat gccaccccgg cccagctcgc cctcgcctgg
     4621 tgcgccaaga acccgaacgt gtccacggtc atcaccggcg ccagccgcgt cgagcaggtc
     4681 accgagaaca tgaaggccat cgaggtgctc ggccgcctcg acgacgccac ggcggagcgg
     4741 atcgcctcga tcttctgacc ggcgtgggcg tcgacgtcga gttcctggag ttcgatcccg
     4801 acgaccccgg tgaggtgctg gccgccatgg ccgacctcgc cgggggcgcc ggctggctca
     4861 ccctcgatcc cgccatcgac gaccgcttcc cggcgccgga gcagaacctc ttcggcaaga
     4921 tcgtgtccgg ccgcgggccg gccgtgcccc gtgccacgtg ggtgccggcc gacgtcgagc
     4981 ggcgccggcc cgaaccgatg tcggtgggga tcctccacgg gaccggcgcc gacgccatcg
     5041 gccgcctggc cgagaaggac atcgtgctgc ccgaccgctg gcgggtggtg gccgaccact
     5101 ccaagcgggg cctcgtcgcc tgggtgccga tcgacatccc cctcgaccag gtcctggcct
     5161 ggaccctcac cgcgtcccgc gcgctgaccc gggtcccgct caccggacgt tggcgagccg
     5221 cggtgcatcg ccggtaagac tccccgggtg agcgacccga cggagcaggc gaccttcgag
     5281 cagatgatgg ccctcgagcc gcacggcgcc gacacctacg tcggcgtcgg gccccgctac
     5341 ccgtggggtg gcctgtacgg cggccagatc gtggcccagg cgctgcgggc cgccggcctc
     5401 accgtcgagc ccgagtacct gccgcactcg ctgcacgcct acttcatccg ccccggcgac
     5461 cacaccgagc cgatccgctt cgaggtcgac cgcatccgca acggccgctc gttcgtgacc
     5521 cggcgggtcg tggcccgcca gtccatcggg gccatcttca acctggccgc ctcgttccac
     5581 gtggtcgagg acgaggccga cgtgcaggcc gccaccctct caccggacgc cggatcgccc
     5641 gacgaccacc tctccgacgg gtggagccac gtgttcgacc gccgccccgt gggcgacatg
     5701 cgccgctccg atgcgggcct gtcgcgcgcc tggctgcgca tggtcgagcc ggtcgccgac
     5761 gaccccctgc tgcgggcgtg cgccatctcc tacgtctccg acgacctccc caccgatggg
     5821 gccatcgccc tgcaccccga ccgcaagctg gtccgcgagg agggcgactg ggagcgcacg
     5881 ttcatgaacg ccagcctcga ccacgccatc tggttccacc gcacggtcga tccggccgag
     5941 tggcacctgc acgagctgcg ggggcacaac ctcaccggcg ctcgcgggct ggccatcggc
     6001 gaggtcttct cccaggccgg cgtgcacgcc gcgacggtcg tgcaggaggt cctcctgcgc
     6061 cgcgtccgca gctgatccga tgatcgcggt gctggccgca gcgctcgtcg cgctgctcat
     6121 cggtctggtc gggaccagct acctgatcgg gtggttccgg cgccacggca tcggccaacc
     6181 gatccacacc gccgtgacgc agcacgcggc caaggcgggc acgccgacca tgggcggcct
     6241 gtcgatcgtc ctggcggcac cgatcggcta cggcgtcggg ctcgcggtgc tgggccgggc
     6301 gccgacccgc accggcatcg tgctcgtcgt cgccatcctc gccggcggcg cggtcggtgt
     6361 cctggacgac tggatgaaga tcagccgcgg acggaacacg ctcgggctgc aggagcggca
     6421 gaagacgctg ctgatcctgg ccgtgggcct gctcctcttc gtcacccagt cctccgacga
     6481 gacatcggcg tgcacggcac cctcgctcgc ccgctgcgag cccttcgccg acctccccgg
     6541 accgctctgg ctgctgtggg tgctcggcgt ggtctggctc acgacgaact cggtcaactt
     6601 caccgacggg ctcgacggcc tgctggccgg cacctcgatc gcgccgctcg ccctgctgag
     6661 cctcatcgcg ttctggcagt tccgccacca gccggcgtac ccggcatccg agggggccct
     6721 cgacgtcgcc ctggtgatgg tggccgtcac ggcgtgctgc gcggggctgc tctggtggag
     6781 cgccgcgccc gcccgggtgt tcatgggcga gaccggctcg ctcgccatcg gcaccgccat
     6841 cgccgtcgcc gcgctcctgt tgcacgtcga cctgctgatc ccggtcttcg gcgccctcta
     6901 cgtcgccgag ggcctgtcga gcttcgccca gcgcgtctgg ttcaaggcga cccgccggtt
     6961 ccgtcgggac cacgagccgc agcggctctt ccgcatggcc ccgctccacc accacttcga
     7021 gctcgtcacg cccgagacca cggtggtcat ccggttctgg atcgtcagcg ccatcgcctc
     7081 ggcgggggcc ggcgccatct tctactggga cgcccttcgc gtcctcgagt agccccaacc
     7141 gggcagtcgg gcacgctgcg catgaggcgt gctccgcggc ccagaacgat cagcgagggg
     7201 cgggttgggc gccgcggacc aggcgaccgg gcagctcgcc cgtggcctcg ccgtcggcgt
     7261 aggtctcgac gccggccacc acggtgtggc ggtacccggt ggcccgctgc agcacccgac
     7321 gtccgccggc ggggaggtcg tggtgcatct cggggcggga gacggcgagg cgatcgaagt
     7381 cgatcacgtt gacgtcggcg cgcatgccgg gggcgaggac gccgcggtcg agcaggccca
     7441 cggtgcgggc ggtgtcctgg gtgtgctggc ggaccaggta ctcgatgtcg aacagctcgc
     7501 cgcgctggcg atcccggccc cagtggctga gcaggaacgt cgggaaggag ccgtcgcaga
     7561 tggtgcccac gtgggcgccg ccgtcggaca ggccggggac ggtgcggggg tgggccagca
     7621 tctcccgggt ggtgtccagg ttgccctcgg cgtagttgag gaacggcacg tagaacatgg
     7681 ccgcgccgcc gtcgccgagc atgaagtcgt aggcccactc gaccgggccg accccggcgc
     7741 gctgcgcctg gccggtcacg gcgtcgtcga gatcgggttc gtactcgggc ggatcgtcga
     7801 gcgggaacat gcggtcgaag gcaccgatga gcccgccgcc gagcttgccc ttggccttgc
     7861 ccgcctcgtg ggcctcgagc acccggcgcc ggaactccgg gtcggacatg gtggcgacct
     7921 gctcggccag cggccggtcg gcgacctcgc gccagacggg gttcgtcagg aacgggttga
     7981 gggtgcagtc gagcccgagc agcaggccga cggcgcgggg cgcgacctgg gctcgcatgt
     8041 cgaagccgtc gtcgaccgca gcgtcgaggc gcttgagcag ctcgcggaac tggtcgggag
     8101 cgaggggcga ctgggccagg gagaacgaca gcggccggcc cgaggcccgc atcatctcga
     8161 cgagggtgcc gaactcgtgg tcgaggtcga ggaagtcgcc gaccatctgc aggacgccga
     8221 tgcgggtggc gcccacggcc tcggcgatg
//
LOCUS       DATISU010000432         2658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_132839, whole genome shotgun sequence.
ACCESSION   DATISU010000432 DATISU010000000
VERSION     DATISU010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132839"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VLR27_17200"
     CDS             complement(<1..638)
                     /locus_tag="VLR27_17200"
                     /inference="COORDINATES: protein motif:HMM:NF005928.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HSP05246.1"
                     /translation="MADICDHDPSDGPLDPVDVLDRIVYLLDRRLEEGRRVRAYANAR
                     DLVAELDHDELLARHRERTLTELAGIGPATSAVIAEALDGRVPKKLVELEATNPVDVG
                     EGAELRAALKGDCHLHSNWSDGGAPIEKMAEAARALGHEYMVLTDHSPRLTIAHGLDP
                     DRLREQLDVIDELNGRWDDFRILTGIEVDILADGTLDQDEDLLDRLDVVVASV"
     gene            679..1023
                     /locus_tag="VLR27_17205"
     CDS             679..1023
                     /locus_tag="VLR27_17205"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HSP05247.1"
                     /translation="MRCPVCLDADLVPHIRDGIEIDHCHRCRGMWLDRGELEKLLDLV
                     EARSPEVRIATHEPPRTPVPPPADLRRHERDDDRDRYVDRDRDDDRHRGRKRKRKGAF
                     AEMLEEVFEAFD"
     gene            complement(1073..1651)
                     /locus_tag="VLR27_17210"
     CDS             complement(1073..1651)
                     /locus_tag="VLR27_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05248.1"
                     /translation="MVFRRRDREPTPDPLADLDPASVGPARRGDALAALDAARRYDAI
                     VGRTPAGPVRDRLESLRAEVHAAVRAVFDAAERTDRKAATLGDLGPEEITRRLKAARG
                     ALARAEDDGRDTSDLRAAAESLDRQLASVHAIWDAVERSADELHRLQLRLGEVVASAG
                     AVAVDVPDRAVDRIGDVADELHALRLALIDLS"
     gene            1692..>2658
                     /gene="ychF"
                     /locus_tag="VLR27_17215"
     CDS             1692..>2658
                     /gene="ychF"
                     /locus_tag="VLR27_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HSP05249.1"
                     /translation="MERFGFVGLPNAGKSSLYNALSGGGALAAPYAFATTDPNVGVAK
                     VPDHRLGLLAEMSKSRNVVPASVQFVDIGGLVEGASQGEGLGNQFLAHIREADAIVFV
                     LRAFEDDDVPGPSDPLEHLRILEIELTLADLETVEKRLDKQRRMFKGDKSLAGEIAVL
                     EEAVAVLGEGTPIYRSAMKPAAREELRQHFLLTNRPVIAIVNVGDDQLDDMDAAVKPV
                     VDELGGVGEVIGMCVQLEAEAATLDTEEERVEMLEGLGLGEGALPRFLHTAYQLMGLR
                     TYFTTGEKESRAWTFRAGSKAPEAAGVIHTDFQRGFIRAECIQWDE"
BASE COUNT          370 a          946 c          976 g          366 t
ORIGIN      
        1 acgctggcca cgacgacgtc gaggcggtcg agcaggtcct cgtcctggtc gagggtgccg
       61 tcggcgagga tgtcgacctc gatgccggtg aggatccgga agtcgtccca gcgcccgttg
      121 agctcgtcga tcacgtcgag ctgctcgcgc aggcggtcgg ggtcgaggcc gtgggcgatg
      181 gtcaggcggg gggagtggtc ggtgaggacc atgtactcgt ggccgagcgc tcgggccgcc
      241 tcggccatct tctcgatggg agcgccgccg tcggaccagt tcgagtggag gtggcagtcg
      301 cccttcagcg ccgcccggag ctcggcgccc tcgccgacgt cgaccgggtt ggtcgcctcg
      361 agctcgacga gcttcttggg cacccgtccg tcgagcgcct cggcgatcac cgcgctcgtc
      421 gcaggaccga tgccggccag ctcggtgagc gtgcgctccc ggtggcgggc gagcagctcg
      481 tcgtggtcga gctcggcgac caggtcgcgg gcgttcgcgt aggcccgcac ccgccggccc
      541 tcctcgaggc ggcggtcgag caggtagacg atgcggtcga gcacgtcgac ggggtccagc
      601 gggccgtcgg acgggtcgtg atcgcagatg tcggccacgc ccgaacgcta tcgccggccg
      661 ccactaccct cgccgggcat gcgctgcccc gtgtgcctcg atgccgacct cgtgccgcac
      721 atccgcgacg ggatcgagat cgaccactgc caccgctgcc ggggcatgtg gctcgaccgg
      781 ggcgagctcg agaagctgct cgacctggtc gaggcccgct cgcccgaggt ccgcatcgcc
      841 acccacgagc cgccgaggac cccggttccc ccgccggcgg atctccgtcg tcacgagcgt
      901 gacgacgacc gggatcgcta cgtcgatcgg gatcgcgacg acgaccgcca ccggggcagg
      961 aagcgcaagc gcaagggggc cttcgccgag atgctcgaag aggtcttcga ggcgttcgac
     1021 tgatcgagaa ctggcagtgc tcctgtagcg cgcaacgcca cgactgccag tctcaggaga
     1081 ggtcgatgag ggcgaggcgc agggcgtgga gctcgtcggc gacgtcgccg atacggtcca
     1141 cggcccggtc gggcacgtcg accgccacgg ctccggcgga ggccacgacc tcgccgaggc
     1201 gcagctgcag gcggtgcagc tcgtcggccg agcgctccac cgcgtcccag atggcgtgga
     1261 ccgaggcgag ctggcggtcg agcgactcgg cggcggcccg caggtcggag gtgtcgcggc
     1321 cgtcgtcctc ggcccgggcc agcgcacctc gagccgcctt cagccggcgg gtgatctcct
     1381 cgggcccgag gtcgccgagg gtggccgcct tgcggtcggt ccgctcggct gcgtcgaaca
     1441 cggctcggac ggcggcgtgg acctcggccc ggagcgattc gagccgatcc cggaccggtc
     1501 cggccggggt gcggccgacg atggcgtcgt agcggcgggc cgcgtcgagg gcggcgagcg
     1561 cgtcgccccg ccgcgccggc ccgacggacg ccggatcgag gtcggcgagg ggatccgggg
     1621 tcggctcgcg atccctgcgc ctgaacacca tgacggggga cgataccgac gccggtagcc
     1681 tcgccatccg catggaacgc ttcggattcg tcggcctccc caacgcgggg aagtcctccc
     1741 tctacaacgc cctctccggg ggcggggccc tggccgcgcc gtacgccttc gccaccaccg
     1801 accccaacgt cggcgtggcc aaggtgcccg accaccgcct cgggctgctc gccgagatgt
     1861 ccaagtcgcg caacgtcgtg ccggcgtcgg tgcagttcgt cgacatcggc gggctggtcg
     1921 agggcgcgtc ccagggtgag ggcctcggca accagttcct ggcccacatc cgcgaggccg
     1981 atgccatcgt cttcgtcctt cgagcgttcg aggacgacga cgtgcccggc ccgtccgacc
     2041 cgctcgagca cctgcgcatc ctcgagatcg agctcaccct cgccgacctc gagaccgtcg
     2101 agaagcgcct cgacaagcag cgccgcatgt tcaagggcga caagtccctc gccggcgaga
     2161 tcgccgtgct cgaggaggcg gtggccgtcc tcggcgaggg gacgccgatc taccgctcgg
     2221 ccatgaagcc ggcggcccgc gaggagctgc gccagcactt cctgctcacc aaccgcccgg
     2281 tgatcgccat cgtcaacgtg ggcgacgacc agctggacga catggacgct gcggtcaagc
     2341 cggtcgtcga cgagctcggc ggggtcggtg aggtcatcgg catgtgcgtc cagctcgaag
     2401 cggaggccgc caccctcgac accgaggagg agcgggtgga gatgctcgag ggcctcgggc
     2461 tgggcgaggg cgcgctgccc cgcttcctgc acaccgccta ccagctcatg ggcctgcgca
     2521 cgtacttcac cacgggcgag aaggagagcc gggcgtggac gttccgggcc ggctccaagg
     2581 cgcccgaggc cgccggcgtc atccacaccg acttccagcg cggcttcatc cgggccgagt
     2641 gcatccagtg ggacgagc
//
LOCUS       DATISU010000433        16954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_831001, whole genome shotgun sequence.
ACCESSION   DATISU010000433 DATISU010000000
VERSION     DATISU010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 16954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16954
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831001"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VLR27_17220"
     CDS             <1..273
                     /locus_tag="VLR27_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HSP05250.1"
                     /translation="AIAARDGGCVFPGCGRPPRHCDIHHLHHRSRGGPTRTGNGAMLC
                     RFHHRLIHHHGWRLLVEDGGWVAVDAHGTRWTGRPTSLVSARGDPP"
     gene            408..1349
                     /locus_tag="VLR27_17225"
     CDS             408..1349
                     /locus_tag="VLR27_17225"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP05251.1"
                     /translation="MSTTSDGPEGAGGTVWSDPAAWAARRTPAGCAICRAGRPHSVLA
                     ALETCWVTGALEAPLPGYVCVVARGHHNEPFEMAPDQQARFWQEAMAVAAAVAGVVQP
                     VKVNYEIHGNTLPHLHLHVFPRQVDDPFVGGPIDPRRSSYVRTDAELEALASAVRRLE
                     PSPSQAGPRDALPNLRVSAVTLSTPDVSRLAGFYERLLGWDRVDDSPGWVRLRSPAGG
                     TGLSFHHDEHFRRPVWPPVEDEQQAMAHLDIATDDLEAAVARAVDLGATTADHQPQRS
                     VRVLLDPDGRPFCLFIGPGRGEHDSVAWHGVDADGQR"
     gene            complement(1357..2364)
                     /locus_tag="VLR27_17230"
     CDS             complement(1357..2364)
                     /locus_tag="VLR27_17230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSP05252.1"
                     /translation="MSDLAGWDVLPDAVLAVAPDGTLAAGNAAATDLLGALDDRIGRP
                     AVEALVVRDPVGDPIAADGWPAPGDEVELLMARADGSDVAVTVTARAVGGGTVLAARP
                     VGDRSGIAIVSTVSHELRSPLTSVKGYVSLLLNRWDRIDDDKKREMLTQVHDDADRVT
                     RLVTELLDISRLETGRLVLRRQLVDVAELATTVVDKVAMSHPDLDCEVRVADDVPRVY
                     ADPDKLEQVLTNLVENAAKYGSLQGMAVTAGLDGPAVRVSVSDTGEGIPADDLPRVFT
                     KFFRRDHGRPSGTGLGLWISRGLVEAHGGELVATSEPGQGATFTFTLPTDAFERAHGE
                     T"
     gene            complement(2361..3149)
                     /locus_tag="VLR27_17235"
     CDS             complement(2361..3149)
                     /locus_tag="VLR27_17235"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HSP05253.1"
                     /translation="MTDLLGPRSSALQHLRRLSGRRRARLDAGQFVIDGPTLVAEAIG
                     AGADVVEVYAEPGAPSDVVAAARAAGIPVRDVESGALAKVTSPVTPQPLAALAGLPAV
                     PDDSVLQGLVLVLVGVADPGNAGTLLRVAEASGASAVVSCADAVDLWNPKCVRASAGA
                     LFRVPILSAGDAAEAVARLRDAGMTVLATTLDDATALDDVDLTGPVAVLLGNEAHGLP
                     ADVVDAADVAVRIPMAGRVESLNVAMTGTVIAFEAARQRRVSAP"
     gene            complement(3161..3541)
                     /gene="rplT"
                     /locus_tag="VLR27_17240"
     CDS             complement(3161..3541)
                     /gene="rplT"
                     /locus_tag="VLR27_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HSP05254.1"
                     /translation="MARVKRGVHSKKRRKQVLGRAKGYYGDKSRTFRSANEQVMHSGN
                     YAFRDRRARKGEMRKLWIQRINAACRTHGTTYSRFVAGLREAGVEVDRKHLADLAVRD
                     DAAFGALVGVADQALGAAQSEGAA"
     gene            complement(3578..3772)
                     /gene="rpmI"
                     /locus_tag="VLR27_17245"
     CDS             complement(3578..3772)
                     /gene="rpmI"
                     /locus_tag="VLR27_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HSP05255.1"
                     /translation="MPKMKTHRTSAKRFKITGSGKVSRRQANRHHGNERMSEKRLRRL
                     KGDELVSKADEKRVKRMLGI"
     gene            complement(3829..4434)
                     /gene="infC"
                     /locus_tag="VLR27_17250"
     CDS             complement(3829..4434)
                     /gene="infC"
                     /locus_tag="VLR27_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HSP05256.1"
                     /translation="MAADEPRINDRIRAREVRLVGADGEQIGIKPLPEALAIARQQDL
                     DLVEVADKANPPVCRIMDYGKYKYEAAQRAKESRRKSSSASIKEMKYRPKIGPGDFDT
                     KTRKVIHFLEEGHKVKITLMFRGREMTHPELGRKILDRVVEATAQVGRVETTARQEGR
                     QMTMVLGPDKKAQAAAKAAAKEADAEAAAEAEADAPSETSD"
     gene            4774..5208
                     /locus_tag="VLR27_17255"
     CDS             4774..5208
                     /locus_tag="VLR27_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05257.1"
                     /translation="MSSLWTPGGEVPVDRSRGGSEPPPAEPAGPRSAPGGQPSEEELA
                     AQAAELQRFLLEAPAEEIVAQHAMGLYELAVIHLSQPEPRLDDARLAIDALAALADGL
                     EGRLGEVGAQLRQALPQLQMAFVQIADRSEGGQDPATDRADG"
     gene            5245..6948
                     /locus_tag="VLR27_17260"
     CDS             5245..6948
                     /locus_tag="VLR27_17260"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSP05258.1"
                     /translation="MADVETRAVHTALADHLNDLVAVFDVDGRCLFLNRTGRRLLRLE
                     PDAPLDLRLGDLPGSADRERFANVVSAVRAGDTWTGSMSMASPITGEFPTRSTICPLT
                     LQSGEPGFGWVAKDTTTDRDVIRSLRQRAFYDPLTGLSHRSLFLDRLDLLLRRVSDDP
                     TPVAVLFVTPDRFKEKINRFDQAVGDALLKAIAGRFPEHLGPDQSVHRWGDEDFVVLC
                     EGVQDEAEALAVAEEVARCFRDPFTVGEHVVFLTASCGVALGVPGTMTTDELIRHADA
                     AGQLAKNTGGGSIRLFDEELRARAMRRAELEDELWGAAERGELVLHYQPEVRLLTNEI
                     VGAEVLVRWRHPRWGLVAPNEFIPIAETSSLIIEIGDWVLREALTQCAALRQRHRDRD
                     ALVIAVNISAKQFVAPDFVDKVAAVLADTGARPADVCLEITESVLLDDAEETISRLGQ
                     LKALGIHLAIDDFGTGYSSLSYLRRFPVDILKVDQSFVSGLGHDPEDSAIVQAVIQMG
                     KALRMTTLAEGVETAHHLIELRELECDVAQGFHFARPVPLEQYEALLDAGDDWLTSST
                     G"
     gene            complement(6955..7179)
                     /locus_tag="VLR27_17265"
     CDS             complement(6955..7179)
                     /locus_tag="VLR27_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05259.1"
                     /translation="MPVSTMGTGAGTVTAFDEDRGLGEITTDGGEVHHFHCTGIADGS
                     RTIEVGTHVRFGIIPGRLGRWEAWEIAETS"
     gene            complement(7182..8057)
                     /gene="hisG"
                     /locus_tag="VLR27_17270"
     CDS             complement(7182..8057)
                     /gene="hisG"
                     /locus_tag="VLR27_17270"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HSP05260.1"
                     /translation="MLKLVLPKGSLEKATMELFADADLQVNRSSSVDYKASVDDPRID
                     EVRILRPQEIPSYLSEGLFDLGIAGRDWVEETSADVVSLGQLQYSKATSNPVRIVVAV
                     PQDSPVQSVADLPQGVRVSSEYPRLTERFFADKGIEADIRLSYGATEAKVPDIVDCVV
                     DITETGRALRAAGLKIVDTILTSYTELLANPASAADPEKAHAMAQLLTLLNGSLEARG
                     KVLVKLNVGGGDLDKVIEIIPSMKAPTVNELYGGGGYAVETVVPKNQINRLIPALVDA
                     GASDILELPLSKIVH"
     gene            complement(8106..8588)
                     /gene="ribH"
                     /locus_tag="VLR27_17275"
     CDS             complement(8106..8588)
                     /gene="ribH"
                     /locus_tag="VLR27_17275"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HSP05261.1"
                     /translation="MASGDDRAQVPEDLDGVGLRIGLVRSRWNAEIVERLCAGVERGL
                     LHLGIPFDDIVIETVPGSFEIPMACRVLARSGEVDAIVTIGAVIRGETTHYELVSEGA
                     ANGVQQVQLETGVPIAFGLVTVENTEQALARSEGPGGHNVGEEAALVAVEMARLAQRH
                     "
     gene            complement(8591..9811)
                     /locus_tag="VLR27_17280"
     CDS             complement(8591..9811)
                     /locus_tag="VLR27_17280"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HSP05262.1"
                     /translation="MPFAPIEDAVAAIGRGEIVVVVDDEDRENEGDLIMAAEYATPEA
                     IAFFLHHTSGYICAPVTEQRAHELELDLMVRENTESMRTAFTVSVDVRQGTTTGISAH
                     DRAATIKALVDPSTRPLDLARPGHINPLIAREGGVLKRAGHTEAAVDLARMAGLYPAG
                     VLCEIVDENKMDMARVPELERFAEKHGLLLISIADMIRYRRKTEKLVKRVAEARIPTE
                     WGDFTCYVYESTLDGEEHVALVRGNVAGEQDVLVRVHSECLTGDAFGSLRCDCGPQLH
                     KAMAQIAAEDQGVVVYLRGHEGRGIGIGHKIRAYSLQEQGQDTVDANTSLGLPVDSRE
                     YGIGAQMLVDLGVTTMRILTNNPSKRGGLEGFGLTITERVPIESVPNPENIKYLRTKR
                     ERMGHLLEGLDDVL"
     gene            9895..10386
                     /locus_tag="VLR27_17285"
     CDS             9895..10386
                     /locus_tag="VLR27_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05263.1"
                     /translation="MTAPDAPDGPARWTPTDPEPSWLAADRRPEGIDDDTVAAVGKLG
                     EAMEWVERARGRLYDFHQMMGRADLLAGEAVEKLRDCGQDGLADDVQEHLVGRNVLEG
                     RWTFQIVEEFDATYYATFQDGLHHLERELMGGSPHVFESEMKEDRRTPGARHHEQRPP
                     EAG"
     gene            complement(10389..10979)
                     /locus_tag="VLR27_17290"
     CDS             complement(10389..10979)
                     /locus_tag="VLR27_17290"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HSP05264.1"
                     /translation="MFTGIVEELGEIRSFEGATLRIGAETVLEDASIGDSIAVNGCCL
                     TVTAKGTDERGGWWEADVSHETLRRTNLGDSAPGTVVNLERPVRAQDRLGGHIVQGHV
                     DAVGEIVEAVPDLRVRVPLDLLRYLVVKGSVTVDGVSLTVVDVLEDGFTVAVIPHTAE
                     VTTLGRKGPGDAVNLEVDVTVKYVERLLEWHRDVGG"
     gene            complement(10979..11992)
                     /gene="ribD"
                     /locus_tag="VLR27_17295"
     CDS             complement(10979..11992)
                     /gene="ribD"
                     /locus_tag="VLR27_17295"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HSP05265.1"
                     /translation="MAEATDEGWMRASIAAASDVRATAHPNPWVGCTIVADGELVATG
                     ATRPPGQAHAEIAALEAAGERARGATAYVTLEPCSHTGRTPPCADALVDAGIARVVVA
                     VLDPDPRVRGAGVERLRQAGIAVDVGCAAELVEGQLAPYLHQRRTGRPYVVLKLAATL
                     DGRTAAPDRTSQWITGPVARADAHRLRAESDSIVVGAGTVRDDDPTLTTRDAEGPDPE
                     RVVLGRAPEGARVHPCTEWDGPIEALLDDLGGRGHVQVLFEGGATVAGDLHRSGLVDR
                     YVVYLAPALLGGDDAPGLFRGPGAATIADAWRGRIESVTALGDDLRIDLVPHARSTTE
                     GTT"
     gene            12135..12974
                     /locus_tag="VLR27_17300"
     CDS             12135..12974
                     /locus_tag="VLR27_17300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSP05266.1"
                     /translation="MCDDDPLLRCVLTRVIGPIGYEVCAEAESPSEALEVIDRVHADV
                     VVLDLALRDGRGEDLLRHLVAERPETKVVVFSSHVVDHDALLGAGATAVVEKPDFPAL
                     EVVLSDLVHGGSLRQDLRRPVARAVPALDPPAALSLSGLEPWSSFRKAAEGLLSGDAV
                     LALDLIPSGTDAGNWDHVLRTDHRVSLARCAASVRRDQDRVSLSPDGVPLLLAVASHP
                     EAPEAVFGRVRALWSREVDRGTPVGIYGHVRKGMPAGTMLDHAVEAVLFDEPTPADPL
                     RMI"
     gene            complement(13029..13493)
                     /locus_tag="VLR27_17305"
     CDS             complement(13029..13493)
                     /locus_tag="VLR27_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HSP05267.1"
                     /translation="MDHAKILTVSDGVVEGTREDRSGQAVEDHLRANGFEIDDRLVVP
                     DGIESVARALRALADGWAGLLVTTGGTGFGPRDLTPEGTRAVLDREAPGLAEAMRLVS
                     PLGRLSRQTAGTIDAALVLNLPGSTSGCIECLDAVLDVVPHALELLRGDRPH"
     gene            complement(13558..14703)
                     /locus_tag="VLR27_17310"
     CDS             complement(13558..14703)
                     /locus_tag="VLR27_17310"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HSP05268.1"
                     /translation="MTAPRPPRSKRKPKPKAPVDAAGVAVRRAALDVLGRIEEEGAYA
                     NLATNAVLGRSDLPDRDRHFVTELVFGTTRMRRACEHLVTRHRTGGMSPRVAAALRLG
                     AYQLAFGDVAPHAAVSTTVGAAPKPARGLVNAVLRRIADDVAAGIEWPDLATELSYPD
                     WIVTALTERLGEEEAVRSLRAMNHAATTHVRDDGYVQDAASQRVVASVGAGPGELVLD
                     ACAAPGGKATGLAATGASVVAADPSPARARLVVGNRDRLDLALPVVVADAVASPYRDG
                     TFDRVLVDAPCSGLGVLRRRPDARWRIDADAPDRLAELQVRILRDAARVVRPGGSLVY
                     SVCTLTEAENQGVVDAVALDGFDLEGTETMAPDDDADGMYVARWRRR"
     gene            complement(14700..15548)
                     /locus_tag="VLR27_17315"
     CDS             complement(14700..15548)
                     /locus_tag="VLR27_17315"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HSP05269.1"
                     /translation="MRPRSLVYLGTPAMSVPPLRALHAAGFDIRLVVTNPDTRRGRGG
                     KTAPSPVKAAAVELDIPVSHTVDDVLGAGADLGVVVAFGRIIRPHVLAELAMVNLHFS
                     LLPRWRGAAPVERAILAGDERTGVDLMVVEEGLDTGGVYARAELDIGADETAEELRTR
                     LVEAGTELLVSSLESGLGEPTPQVGEPVYAHKLTSDDLRIDWDAPAEQIHRQVRVGGA
                     WTTLDGERFKVWRTSLPPADDGVVHPTGTTPIELLEVQPAGKARMAASAWANGARWSD
                     GDRLGT"
     gene            complement(15565..16107)
                     /gene="def"
                     /locus_tag="VLR27_17320"
     CDS             complement(15565..16107)
                     /gene="def"
                     /locus_tag="VLR27_17320"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HSP05270.1"
                     /translation="MSAPYNIRVVGDPVLRAAAAEITDIDGKLVKLAHDMLDTMYEAP
                     GLGLAAPQIGVGKRLFVYDLDPEERAEPHVLVNPRIVESDGEWTYEEGCLSIPGLSFE
                     IVRPKVVHLVGVDLDGNEVSIEADELTARLFQHELDHLDGVLMTERMDPDTKKQALKE
                     IRRIRDRFEVAPREPSGFFS"
     gene            16135..16779
                     /locus_tag="VLR27_17325"
     CDS             16135..16779
                     /locus_tag="VLR27_17325"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSP05271.1"
                     /translation="MGAPPDQPSTEPTSGGAEGHYFAAAPTARRRPGHIIVDVHGDRF
                     ELATDSATFSPRRLDPGTAFLLDAAPAPPPAGTFLDLGCGYGPIAAVLARTRPDAHVV
                     AVDVNERSRELCARNATDLGCTNVEVLAPDDVDPALRFDLIWSNPPIRIGKDALHELL
                     LRWFERLTPDGEAVLVMGRNLGADSMQRWLGEQGFPTERLASKRGYRLLRSRRS"
BASE COUNT         2224 a         6082 c         6223 g         2425 t
ORIGIN      
        1 gccatcgcgg cacgagacgg cggctgcgtg ttccccggct gcggtcgtcc accacggcac
       61 tgcgacatcc accacctgca ccaccgcagc cggggcggcc cgacccgcac cggcaacggc
      121 gcgatgctct gccgcttcca ccaccgcctc atccaccacc acggttggcg cctgctggtc
      181 gaggacggcg ggtgggtggc cgtcgacgcc cacggcaccc gatggaccgg ccgaccgacc
      241 tcgctcgtca gcgcgcgcgg cgatccgccc tgacgctgca cgccgccgct acccaggccc
      301 cgtccgtccg ctccgatcca ctcacgctcc gaccccagcc ccggtcgacc cgatccccag
      361 atcgggtcac cggcgcgcca cggctgccga cgtgcgagca tcgggccatg tccacgacct
      421 cggacggtcc agagggcgcg ggcggcacgg tctggtcgga cccggcggcg tgggccgcac
      481 gtcgcacgcc cgccgggtgc gccatctgcc gggccggacg cccccacagc gtgctggccg
      541 cgctcgagac ctgttgggtg accggcgccc tcgaggcgcc gctcccgggc tacgtctgcg
      601 tggtcgcacg cggccaccac aacgaaccct tcgagatggc tcccgaccag caggcgcggt
      661 tctggcagga ggcgatggct gtggccgcgg ccgtcgccgg cgtcgtccag cccgtcaagg
      721 tgaactacga gatccacggc aacacgctcc cccacctcca cctgcacgtc ttcccgcgcc
      781 aggtcgacga ccccttcgtc ggcggaccga tcgacccgcg ccggagctcg tacgtgcgca
      841 ccgacgccga gctggaggcg ctcgcctcgg cggtgcgccg cctggagccc tcgcccagcc
      901 aggccggtcc acgagatgcg ctccccaacc tgcgcgtgtc ggcggtgacc ctgtccacgc
      961 cggacgtgag ccgcctcgcc ggcttctacg agcggttgct gggctgggac cgcgtcgacg
     1021 actcgcccgg gtgggtgcgc ctccgctccc ccgccggcgg gaccggcctc tcgttccacc
     1081 acgacgagca cttccgtcga ccggtgtggc cgcccgtcga ggacgagcag caggcgatgg
     1141 cccacctcga catcgccacc gacgatctgg aggccgccgt ggcccgggcc gtcgacctcg
     1201 gcgccacgac cgccgaccac cagccccagc gcagcgtccg ggtgctgctc gacccggacg
     1261 gccggccctt ctgcctcttc atcggccccg gtcgcggtga gcacgactcg gtggcctggc
     1321 acggcgtcga cgccgacggc cagcgctgag cgacgctcag gtctcgccgt gagcccgctc
     1381 gaaggcgtcg gtggggagcg tgaaggtgaa cgtggcgccc tgcccgggtt ccgaggtggc
     1441 gacgagctcg cccccgtggg cctcgaccag gcctcggctg atccacaggc cgaggcccgt
     1501 gcccgaggga cggccgtggt cccggcggaa gaacttggtg aagacgcggg gcaggtcgtc
     1561 ggctgggatg ccctcgcccg tgtccgacac cgacacccgg acggcgggtc cgtcgagccc
     1621 ggcggtgacg gccatgccct ggaggctgcc gtacttcgcc gcgttctcga ccaggttggt
     1681 gagcacctgc tcgagcttgt ccgggtcggc gtagacccgg ggcacgtcgt cggccacccg
     1741 gacctcgcag tcgaggtcgg ggtgggacat ggcgaccttg tccaccacgg tcgtggccag
     1801 ctcggcgaca tcgaccagct ggcggcggag gacgaggcga ccggtctcga gtcggctgat
     1861 gtcgagcagc tcggtcacga ggcgcgtcac ccggtcggcg tcgtcgtgga cctgggtgag
     1921 catctcgcgc ttcttgtcgt cgtcgatgcg gtcccagcgg ttcagcagca gcgagacgta
     1981 gcccttgacc gacgtcagcg gggaccgcag ctcgtgggag acggtggaca cgatggcgat
     2041 gccggagcgg tcgcccaccg gtcgggcggc gagcacggtg ccgcctccga ccgcacgggc
     2101 cgtgacggtg acggcgacgt cgctcccgtc ggcccgggcc atgaggagct cgacctcgtc
     2161 gccgggcgcc ggccacccgt cggctgcgat ggggtccccg accgggtcgc gcacgaccag
     2221 ggcctcgacc gccggacggc cgatccggtc gtcgagcgcg cccagcaggt cggtggccgc
     2281 ggcgttgccc gccgccagcg tcccgtccgg cgccacggcc aggacggcgt cggggagcac
     2341 atcccacccg gcgaggtcgc tcacggtgcg ctcacgcggc gctggcgggc ggcctcgaag
     2401 gcgatgacgg tgccggtcat cgccacgttc agggattcga cccgcccggc catggggatg
     2461 cgcacggcga cgtcggccgc gtcgaccacg tcggcgggca ggccgtgggc ctcgttgccg
     2521 aggaggacgg cgacgggtcc ggtgaggtcg acgtcgtcca gtgcggtggc gtcatcgagc
     2581 gtggtggcca gcacggtcat gcccgcgtcc cgcaggcggg cgacggcctc ggcggcgtcg
     2641 ccggcggaaa ggatgggcac ccggaacagc gcgccggcgg atgcacggac gcacttgggg
     2701 ttccacaggt ccaccgcatc ggcgcaggac actactgccg aggccccgga cgcttcggcg
     2761 acgcgcagga gggtcccggc gttgcctgga tcggcaacgc cgaccagcac cagcaccagg
     2821 ccctggagga cggagtcgtc gggcacggcc ggcaggccgg ccagcgcggc gaggggctgg
     2881 ggcgtcaccg gtgaggtcac cttggccagc gcaccggact cgacgtcgcg cacggggatg
     2941 ccggcggcgc gggccgccgc gaccacgtcg gagggggccc ccggctcggc gtagacctcg
     3001 accacgtcgg cgccggcccc gatggcttcg gccacgaggg tcggtccgtc gatgacgaac
     3061 tggccggcgt ccagacgcgc cctacggcgc cccgagaggc gccgtaggtg ctgcagtgcg
     3121 ctcgaccggg ggccgagcag gtccgtcacg gaccgcgtcg ctatgcggcg ccctcggact
     3181 gggcggcgcc gagggcctgg tcggccacgc cgacgagcgc cccgaaggcg gcatcgtcgc
     3241 ggacggccag atcggccagg tgcttgcggt cgacctcgac cccggcctca cgcagacccg
     3301 cgacgaagcg cgagtaggtg gtgccgtggg tgcggcaggc ggcgttgatg cgctggatcc
     3361 agagcttgcg catctcgccc ttgcgggctc gacggtcgcg gaacgcgtag ttccccgagt
     3421 gcatgacctg ctcgttggcc gagcggaagg tgcggctctt gtcgccgtag tagcccttcg
     3481 cccgacccag tacctgcttg cgacgcttct tgctgtggac gccgcgcttg acacgtgcca
     3541 tgtctgactc ctctcagtga atgtcggctg ttgcttctca gatgccgagc atccgcttga
     3601 cgcgcttctc gtcggccttg ctgacgagct cgtcgccctt gaggcggcgc aggcgcttct
     3661 cgctcatgcg ctcgttgccg tggtggcggt tggcctggcg acggctcacc ttgccgcttc
     3721 cggtgatctt gaagcgcttg gccgacgtgc ggtgggtctt catcttgggc atggctcttc
     3781 cttcgtcttc gacgtgctct ggttgcaggg cggcgatgcg cccggcgttc agtcggacgt
     3841 ctcgctgggg gcgtcggcct cggcttcggc tgctgcctcg gcgtcggctt ccttggctgc
     3901 ggccttcgcc gccgcctggg ccttcttgtc cggtccgagg accatcgtca tctggcgacc
     3961 ttcttgacga gccgtggtct ccacccggcc cacctgggcc gtggcctcca ccactcggtc
     4021 gaggatcttg cggccgagct ccgggtgggt catctctcga cctcggaaca tcagcgtgat
     4081 cttgaccttg tgaccctcct ccaggaagtg gatcaccttc ctggtcttgg tgtcgaagtc
     4141 accgggcccg atcttgggtc ggtacttcat ctccttgatg gaagcactgg agctcttgcg
     4201 acgagattcc ttcgcccgtt gcgcagcctc gtacttgtac ttcccgtagt ccatgatgcg
     4261 acacacgggt gggttggcct tgtccgccac ctcgaccagg tcgagatctt gctggcgggc
     4321 gatggccaat gcctcgggta ggggcttgat cccgatctgc tcaccgtcag cacccacgag
     4381 gcggacctcg cgggcacgga tgcggtcgtt gatgcggggc tcgtcagcag ctattgccga
     4441 tcactcctcg acaagttgtc cgttcctcct gtggttcgga actccgttcg gaatcacgag
     4501 gaagcggtac ctgccgggag ggcgttgccc ccacacgcgg aagcggacgt ccgaagacgt
     4561 ccgccacacc gatgcgcccc cacccggagg tgacggcgtg gtgttccacg acccgagcgc
     4621 accagcggtg gtcgggtggg gggcgggccc cgcttgctcg atcagttgtg gcaccaatgt
     4681 accgggtcgt ggcgccgacg tggcaagtgc gccaggatca acggtccgac ctgcgctgtg
     4741 cacgccaccc cggtggcgag gaaggagatg cccgtgtcat cgctgtggac accaggaggc
     4801 gaggtccccg tcgatcgctc ccggggcggt tcggagccgc cgccagcgga gccggccggt
     4861 ccgaggtccg ccccgggcgg gcagccgtcc gaggaggagc tggccgccca ggccgccgag
     4921 ctgcagcgct tcctgctcga ggcgccagcg gaggagatcg tcgcccagca cgccatgggg
     4981 ctctacgagc tggccgtcat ccacctgtcc cagcccgagc cccgcctcga cgacgcccgg
     5041 ctggccatcg acgccctcgc ggccctcgcc gatggcctcg agggacgtct cggcgaggtc
     5101 ggcgcccagc tgcgccaggc gctcccccag ctgcagatgg ccttcgtcca gatcgccgat
     5161 cgcagcgaag gcggccagga cccggccacg gatcgcgccg acggataggt tctggcacgc
     5221 tggtgcgagc aagggggagg tgacgtggcc gacgtggaga cccgagcggt gcacaccgcg
     5281 ctcgccgatc acctcaacga cctcgtcgcc gtgttcgacg tcgacggccg gtgcctcttc
     5341 ctgaaccgca ccggtcggcg cctcctgcgc ctggaaccgg acgcgcccct cgacctgcgc
     5401 ctcggcgatc tgcccggcag cgccgaccgg gagcgcttcg ccaacgtcgt gtccgccgtc
     5461 cgggccgggg acacctggac ggggtcgatg tcgatggcct cccccatcac cggcgagttc
     5521 cccacccgct ccacgatctg ccccctcacc ctgcagagcg gcgagcccgg cttcggttgg
     5581 gtcgccaagg acacgaccac cgaccgggac gtgatccgta gcctgcgcca gcgggcgttc
     5641 tacgaccccc tcaccgggct gtcgcaccgc tcgctgttcc tcgaccggct cgacctcctg
     5701 ctgcgacggg tgagcgacga cccgaccccg gtcgcggtgc tgttcgtgac gccggaccgg
     5761 ttcaaggaga agatcaaccg cttcgaccag gccgtcggcg acgccctgct caaggccatc
     5821 gccggtcggt tccccgagca cctcggcccc gaccagagcg tgcaccgctg gggcgacgag
     5881 gacttcgtgg tgctctgcga gggcgtgcag gacgaggccg aggccctcgc cgtcgccgag
     5941 gaggtcgccc gctgcttccg tgacccgttc accgtcggcg agcacgtcgt gttcctcacc
     6001 gcgtcctgcg gggtcgccct cggcgtgccg ggcacgatga ccaccgacga gctcatccgc
     6061 cacgcggatg ccgccggcca gctggccaag aacacgggcg ggggcagcat ccggctgttc
     6121 gacgaggagc tgcgggcccg ggccatgcgc cgagccgagc tcgaggacga gctgtggggc
     6181 gcggccgagc gcggcgagct ggtgctgcac taccagcccg aggtgcggct gctcaccaac
     6241 gagatcgtcg gcgccgaggt gctcgtgcgc tggcggcacc cccggtgggg cctcgtcgcc
     6301 cccaacgagt tcatccccat cgccgagacc agcagcctga tcatcgagat cggcgactgg
     6361 gtcctgcgcg aggccctcac gcagtgcgcg gcgctgcggc agcgccaccg ggaccgcgac
     6421 gcgctggtga tcgcggtcaa catctcggcc aagcagttcg tcgcccccga cttcgtcgac
     6481 aaggtcgccg ccgtgctcgc cgacaccggt gcccgccccg ccgacgtgtg cctggagatc
     6541 accgagagcg tgctgctcga cgacgcggag gagaccatct cccggctcgg ccagctcaag
     6601 gccctcggca tccacctcgc catcgacgac ttcggcaccg gctactcgtc gctgtcgtac
     6661 ctgcggcgct tccccgtcga catcttgaag gtggaccaga gcttcgtcag cgggctcggc
     6721 cacgacccgg aggactccgc catcgtgcag gcggtgatcc agatgggcaa ggcgctgcgg
     6781 atgaccaccc tggccgaggg ggtcgagacc gcgcaccacc tgatcgagct gcgcgagctg
     6841 gagtgcgacg tcgcccaggg cttccacttc gcccgaccgg tccccctcga gcagtacgag
     6901 gcgctgctcg acgccggtga cgactggctg accagctcga ccggctgacg ccggctacga
     6961 cgtctccgcg atctcccagg cctcccagcg gccgaggcgg ccggggatga tgccgaagcg
     7021 cacgtgcgtg ccgacctcga tggtgcggct gccatcggcg atgccggtgc agtggaagtg
     7081 gtggacctcg ccgccatcgg tggtgatctc gcccaggccg cggtcctcgt cgaacgccgt
     7141 gaccgtcccg gccccggtcc ccatcgtgct gacgggcatg gtcagtggac gatcttggac
     7201 aacgggagct cgaggatgtc gctggcgccg gcgtcgacca gggcggggat cagccggttg
     7261 atctggttct tcggcacgac ggtctcgacc gcgtagccgc cgccgccgta gagctcgttg
     7321 acggtggggg ccttcatcga cgggatgatc tcgatgacct tgtcgaggtc gccgccaccc
     7381 acgttgagct tcaccagcac cttgccccgg gcctcgaggg agccgttcag cagcgtcagc
     7441 agctgggcca tggcgtgggc cttctcggga tccgcggcgc tggccgggtt ggcgagcagc
     7501 tcggtgtagc tcgtcaggat cgtgtcgacg atcttgaggc cggcggcgcg cagggcccgg
     7561 ccggtctcgg tgatgtcgac gacgcagtcg acgatgtcgg ggaccttggc ctcggtggcg
     7621 ccgtaggaga ggcggatgtc ggcctcgatg cccttgtcgg cgaagaaccg ctcggtgagg
     7681 cgggggtact cgctggagac ccggacgccc tggggcaggt cggccaccga ctggaccggg
     7741 gagtcctggg gcacggcgac cacgatgcgc accgggttcg aggtggcctt ggagtactgc
     7801 agctggccga ggctgacgac gtcggcgctg gtctcctcga cccagtcacg gccggcgatg
     7861 ccgaggtcga agagcccctc ggagaggtag ctcgggatct cctgcgggcg caggatccgg
     7921 acctcgtcga tgcgggggtc gtcgacggac gccttgtagt cgacgctgct cgaccggttc
     7981 acctggaggt ccgcgtcggc gaacagctcc atcgtggcct tctcgagcga tcccttgggc
     8041 agcaccagct tcagcacgcc ggccacggta cgggtgggcc gacgggccga ccgaatccgt
     8101 ttggttcagt ggcgctgggc gaggcgggcc atctccacgg ccacgagggc ggcttcctcg
     8161 ccgacgttgt ggcccccggg gccttcggag cgggccaggg cctgctcggt gttctccacc
     8221 gtgaccagac cgaacgcgat ggggacgccg gtctcgagct gcacctgctg cacgccgttg
     8281 gctgcaccct cggacaccag ctcgtagtgg gtggtctccc cccggatgac ggcgccgatg
     8341 gtgacgatgg cgtcgacctc accggagcgg gccagcaccc ggcaggccat ggggatctcg
     8401 aaggagccgg gcacggtctc gatcacgatg tcgtcgaagg ggatcccgag gtggagcagg
     8461 ccgcgctcga cgcccgcgca gaggcgctcg acgatctcgg cgttccaccg cgagcgcacc
     8521 aggccgatcc gcaggccgac gccgtcgagg tcctcgggca cctgggcccg gtcgtcaccg
     8581 ctcgccatgg ctacaggacg tcgtcgaggc cctcgaggag gtgccccatg cgctcgcgct
     8641 tggtccggag gtacttgatg ttctccgggt tcggcaccga ctcgatcggg acccgctcgg
     8701 tgatggtgag cccgaagccc tcgaggccac cccgcttcga cgggttgttg gtgaggatcc
     8761 gcatggtcgt gacgccgagg tcgaccagca tctgggcgcc gatgccgtac tcgcgggagt
     8821 cgaccgggag gccgagcgag gtgttggcgt cgacggtgtc ctggccctgc tcctgcagcg
     8881 agtacgcccg gatcttgtgg ccgatgccga tgccgcggcc ctcgtggccg cgcaggtaca
     8941 ccaccacgcc ctggtcctcg gcggcgatct gggccatcgc cttgtgcagc tgggggccgc
     9001 agtcgcagcg cagggagccg aacgcgtcgc cggtgaggca ctcgctgtgc acccggacga
     9061 gcacgtcctg ctcgccggcg acgttgcccc gcacgagggc gacgtgctcc tcgccgtcga
     9121 gggtggactc gtacacgtag caggtgaagt cgccccactc ggtggggatg cgggcctcgg
     9181 ccacccgctt gaccagcttc tcggtcttgc gccggtagcg gatcatgtcg gcgatggaga
     9241 tgagcaggag gccgtgcttc tcggcgaagc gctccagctc gggcacccgg gccatgtcca
     9301 tcttgttctc gtcgacgatc tcgcagagca cgccggccgg gtagaggccg gccatgcggg
     9361 ccaggtcgac cgccgcttcg gtgtgcccgg cccgcttcag cacgccgccc tcacgggcga
     9421 tgagcgggtt gatgtggccg gggcgggcca ggtcgagcgg gcgggtgctc gggtcgacga
     9481 gggccttgat ggtggcggcg cggtcgtggg cggagatgcc ggtggtggtc ccctggcgca
     9541 cgtcgacgga gaccgtgaac gcggtgcgca tcgactcggt gttctcccgc accatcaggt
     9601 cgagctcgag ctcgtgggcc cgctgctcgg tgacgggggc gcagatgtag cccgaggtgt
     9661 ggtggaggaa gaaggcgatg gcctcgggcg tggcgtactc ggccgccatg atgaggtcgc
     9721 cctcgttctc gcggtcctcg tcgtcgacga ccacgacgat ctcgcctcgg ccgatggctg
     9781 cgacggcgtc ttcgatgggt gcgaagggca ttgctctcct tccgggggtg ggccgagcgt
     9841 acccgtggcc ccgcggcggg cacccaacgg ggccggccgg gtaggagaga caccatgacc
     9901 gcacctgacg caccggacgg ccccgcccgc tggaccccga ccgacccgga gccgtcctgg
     9961 ctcgccgcgg accgccgtcc cgagggcatc gacgacgaca cggtcgccgc cgtcggcaag
    10021 ctgggcgagg ccatggagtg ggtcgagcgg gctcgcggcc ggctctacga cttccaccag
    10081 atgatggggc gggccgacct gctcgccggc gaggccgtcg agaagctgcg ggactgcggg
    10141 caggacggtc tggccgacga cgtgcaggag cacctggtcg ggcgcaacgt gctcgagggc
    10201 cgctggacgt tccagatcgt cgaggagttc gacgccacgt actacgccac gttccaggac
    10261 gggttgcacc acctcgagcg cgagctcatg ggcggctcgc cccacgtgtt cgagtccgag
    10321 atgaaggagg accgccgcac gccgggcgcc cgccaccacg agcagcgccc acccgaggcc
    10381 ggctgagctc agccgccgac gtcgcggtgc cactccagga ggcgctcgac gtacttcacc
    10441 gtgacgtcga cctccaggtt cacggcgtca cccggcccct tgcgcccgag ggtggtgacc
    10501 tcggcggtgt gggggatgac cgcgacggtg aagccgtcct cgagcacgtc caccaccgtg
    10561 aggctcaccc cgtcgacggt gaccgagccc ttcacgacca ggtagcgcag caggtcgagc
    10621 ggcacccgca cccgcaggtc aggcacggcc tcgacgatct cgccgaccgc gtcgacgtgg
    10681 ccctgcacga tgtgcccgcc gaggcggtcc tgggcccgca cgggacgctc caggttcacg
    10741 accgtgccgg gcgcggagtc gccgaggttc gtccggcgca gcgtctcgtg ggagacgtcg
    10801 gcctcccacc agccgccgcg ttcgtccgtg cccttggcgg tgacggtcag gcagcagccg
    10861 ttgaccgcga tggagtcgcc gatggaagcg tcctcgagca cggtctctgc gccgatgcgc
    10921 agggtggcgc cttcgaagga gcggatctcg cccagctcct ccacgatgcc ggtgaacatc
    10981 aggtggtccc ttcggtggtg gatcgggcgt gcggcacgag gtcgatgcgc aggtcgtcgc
    11041 ccagcgccgt gaccgattcg atgcggccac gccaggcatc cgcgatggtg gcggcaccag
    11101 gaccgcggaa caagccgggg gcgtcgtcgc cgccgagcag cgccggcgcg aggtagacga
    11161 cgtagcggtc gacgagcccc gagcggtgca ggtcaccggc gacggtggca ccgccctcga
    11221 acagcacctg gacgtgccct cgtccgccca ggtcgtcgag cagcgcctcg atcggaccgt
    11281 cccactcggt gcacgggtgc acccgggcgc cctcgggagc acggccgagg acgacgcgct
    11341 cggggtccgg accctctgca tcgcgggtcg tgagggtggg gtcgtcgtcg cgcaccgtgc
    11401 cggcgccgac gacgatgctg tcgctctcgg cccggagccg gtgggcatcg gcacgggcaa
    11461 cgggcccggt gatccactgg ctggtgcggt ccggagcggc ggtgcgcccg tcgagggtgg
    11521 cagccagctt cagcacgacg tagggccgac cggtgcggcg ctggtgcagg tacggggcga
    11581 gctgtccctc gaccagctcg gcggcgcagc cgacatcgac cgcgatcccc gcctggcgga
    11641 ggcgctcgac cccggcgccc cggacccgag ggtcgggatc gagcaccgcg acgacgaccc
    11701 gcgcgatgcc ggcgtcgacg agcgcatcgg cgcatggcgg cgtgcggccg gtgtggctgc
    11761 acggctcgag ggtcacgtag gccgtggcac ccctggcccg ctcccccgcc gcttcgaggg
    11821 cggcgatctc ggcgtgggcc tggccgggcg ggcgggtggc gccggtggcg acgagctcgc
    11881 cgtcggccac gatcgtgcag cccacccacg ggttcgggtg ggcggtggcc cggacgtcgg
    11941 acgcggcggc gatcgacgcc cgcatccagc cctcgtcggt cgcctcagcc accgggcgag
    12001 cgtacaagcg gccccgaagc tcgccccatc ccccgccgct gtcgccgatc tcgccagaat
    12061 gtccgaagat ccaggaccgg cctggggtgc acgggttcga gcgagggagg gcgatgtcgt
    12121 cgaggtctgc ggtcgtgtgc gatgacgacc cgctgctgcg ctgcgtgctc acccgggtca
    12181 tcgggccgat cggctacgag gtctgcgccg aggccgagag cccgtcggag gcgctggagg
    12241 tcatcgaccg cgtccacgcc gacgtcgtcg tgctcgacct ggccctccgc gacggcaggg
    12301 gcgaggacct gctgcgccac ctcgtcgccg agcgcccgga gaccaaggtc gtcgtgttct
    12361 cgtcccacgt cgtcgaccac gacgccctgc tcggcgccgg cgccacggcc gtggtggaga
    12421 agcctgactt ccccgccctc gaggtggtgc tcagcgacct cgtccacggc ggctcgctcc
    12481 gccaggacct ccgccggccc gtcgcccggg cggtgccagc gctcgatccg ccggcagccc
    12541 tgagcctcag cggcctggag ccgtggtcgt cgttccgcaa ggccgccgag gggctcctga
    12601 gcggcgatgc cgtgctggcc ctcgacctga tcccgtccgg gacggacgcc ggcaactggg
    12661 accacgtcct gcgcaccgac caccgggtct ccctcgcccg ctgcgctgcc tccgtgcgcc
    12721 gcgaccagga ccgggtgagc ctctcccccg acggcgtccc cctgctgctg gcggtcgcct
    12781 cacacccgga ggcacccgag gcggtgttcg gccgggtccg ggcgctgtgg tcccgtgagg
    12841 tcgaccgcgg gacccccgtg ggcatctacg ggcacgtgcg caagggcatg cccgccggga
    12901 cgatgctcga ccacgccgtc gaggccgtgc tgttcgatga gccgacgccg gccgacccgc
    12961 tgcggatgat ctgaccggac cgggacgtcc cacacgcctc cggggcgtgc tgcgcggccc
    13021 ggaacggatc agtgggggcg gtcgccgcgg aggagctcga gcgcgtgggg gacgacgtcg
    13081 aggacggcgt cgaggcactc gatgcagccg ctggtggacc ccggcaggtt caggaccagg
    13141 gcggcgtcga tggtgccggc cgtctggcgg ctcagccggc cgagcgggct gacgaggcgc
    13201 atggcctcgg ccaggccggg tgcctcgcgg tcgagcacgg cccgggtgcc ctcgggcgtg
    13261 aggtcccgtg ggccgaagcc ggtgccgccg gtggtgacga gcaggccggc ccagccgtcg
    13321 gccagggcgc gcagggcccg ggcgaccgac tcgatgccgt cggggacgac gaggcggtcg
    13381 tcgatctcga agccgttggc ccgcaggtgg tcctcgacgg cctggccgga gcggtcctcg
    13441 cgggtgccct cgacgacgcc gtcggagacg gtgaggatct tggcgtggtc catcggcgga
    13501 ccagcctacg gacgacccca ccccggctcc gcgatggcgt ctgacctggg cagacgccta
    13561 ccggcggcgc caccgggcga cgtacatgcc gtcggcatcg tcgtcgggtg ccatggtctc
    13621 ggtgccttcg aggtcgaagc cgtccagcgc gaccgcatcg accacaccct ggttctccgc
    13681 ctcggtgagc gtgcacacgc tgtagaccag cgaacccccc gggcgcacca cgcgggcggc
    13741 gtcgcgcagg atgcggacct ggagctcggc cagccggtcg ggcgcgtcgg cgtcgatgcg
    13801 ccagcgggca tcgggacgtc ggcggagcac accgagaccg gagcacgggg cgtcgaccag
    13861 gacccggtcg aaggtgccgt cccggtacgg ggacgcgacg gcgtcggcca cgacgaccgg
    13921 cagcgccagg tccaggcggt cgcggttgcc caccacgagg cgggcccgcg ccggggacgg
    13981 gtcggcggcc accacggagg caccggtggc ggcgaggccg gtggccttgc ccccgggggc
    14041 ggcgcacgcg tcgaggacga gctcgccggg gccggcaccg accgacgcga cgacccgctg
    14101 ggaggcggcg tcctgcacgt agccgtcgtc tcggacgtgg gtggtggcgg cgtggttcat
    14161 ggcgcgcagg gagcggaccg cctcctcctc gccgagccgt tccgtgaggg cggtgacgat
    14221 ccagtcgggg taggagagct cggtggccag gtcgggccac tcgatccccg cggccacatc
    14281 gtcggcgatg cggcggagca cggcgttcac gagaccccgg gcgggcttcg gcgcggcgcc
    14341 gaccgtggtg ctcacggcag cgtggggggc gacgtcgccg aacgcgagct ggtaggcgcc
    14401 gaggcgcagc gcggccgcga cccggggcga catgccgccg gtgcggtgcc gggtcacgag
    14461 gtgctcgcac gcccggcgca tgcgcgtggt gccgaagacc agctcggtga cgaagtgccg
    14521 atcgcggtcg ggcaggtcgc tgcggccgag caccgcgttg gtcgccaggt tggcgtaggc
    14581 gccctcctcc tcgatgcgac cgaggacgtc gagagcggcc cggcgcaccg ccaccccggc
    14641 ggcgtcaacg ggggccttgg gcttcggctt ccgcttggac ctcggcgggc ggggcgcagt
    14701 cacgtgccga gccggtcgcc gtcggaccag cgggcgccgt tggcccacgc cgatgccgcc
    14761 atgcgggcct tgccggcggg ctggacctcg agcagctcga tgggcgtggt gccggtcggg
    14821 tggaccacgc cgtcgtcggc cggcgggagc gaggtgcgcc agaccttgaa gcgctcaccg
    14881 tccagggtcg tccacgcacc tccgacgcgc acctggcggt ggatctgctc cgccggcgcg
    14941 tcccagtcga tgcgcaggtc gtcggaggtg agcttgtggg cgtacaccgg ctctcccacc
    15001 tgcggggtcg gctcgcccag gccggactcc agcgacgaga cgagcagctc ggtgccggcc
    15061 tcgaccaacc tcgtccggag ctcctccgcc gtctcgtcgg cgccgatgtc gagctcggcg
    15121 cgggcgtaca cgccaccggt gtcgaggccc tcctccacga ccatgaggtc cacgccggtg
    15181 cgctcgtcgc cggccaggat cgcccgctcg accggggcgg ctccccgcca gcgcggcagc
    15241 agggagaagt gcaggttgac catcgccagc tcggcgagca cgtggggccg gatgatgcgg
    15301 ccgaaggcca ccacgacgcc caggtcggca ccggcgccga gcacgtcgtc gacggtgtgg
    15361 gacacgggga tgtcgagctc gacggctgcg gccttcaccg ggctcggtgc ggtcttgccg
    15421 ccgcggccgc gacgggtgtc ggggttggtc acgacgaggc ggatgtcgaa gccggcggcg
    15481 tgcagggcgc gcagcggcgg caccgacatg gccggggtgc cgaggtagac gagcgagcgg
    15541 gggcgcacgg ggcgggggcc gcggctagga gaagaaaccg gacggctccc ggggggcgac
    15601 ctcgaagcga tcgcggatgc gccggatctc cttcagggcc tgcttcttcg tgtcggggtc
    15661 catccgctcg gtcatgagca ccccgtcgag gtggtccagc tcgtgctgga acagccgggc
    15721 ggtgagctcg tcggcctcga tggagacctc gttgccgtcg aggtcgacgc cgaccaggtg
    15781 cacgaccttg ggtcgcacga tctcgaagga cagccccggg atcgacaggc agccctcctc
    15841 gtaggtccac tcgccgtcgg actcgacgat gcgggggttg accagcacgt ggggctcggc
    15901 ccgctcctcg ggatcgaggt cgtagacgaa gaggcgcttg cccaccccga tctggggggc
    15961 ggccaggccg aggcccgggg cctcgtacat ggtgtcgagc atgtcgtggg cgagcttgac
    16021 cagcttgccg tcgatgtcgg tgatctcggc ggcggcggcg cgcagcaccg gatcgccgac
    16081 gactcggatg ttgtagggcg cgctcatgac gggcccaacg ctaccgtcgc cgccgtgggc
    16141 gccccaccgg accagccgtc gaccgagccc acctccggtg gcgccgaggg tcattacttc
    16201 gccgcagcgc cgacggcgcg ccgccgaccc ggccacatca tcgtcgacgt gcacggcgac
    16261 cgcttcgagc tggcgaccga ctcggccacc ttcagccccc gccggctcga ccccggcacc
    16321 gcgttcctcc tcgatgccgc cccggcgccg ccgccggcgg gcacgttcct cgacctcggc
    16381 tgcggctacg gaccgatcgc cgccgtgctc gcccggacgc gccccgacgc ccacgtcgtc
    16441 gccgtggacg tgaacgagcg cagccgcgag ctgtgcgccc ggaacgccac cgacctcggc
    16501 tgcaccaacg tcgaggtgct cgcccccgac gatgtcgacc ccgccctccg cttcgacctc
    16561 atctggtcga acccgccgat ccgcatcggc aaggacgcgc tccacgagct gctgctgcgc
    16621 tggttcgagc ggctcacccc ggacggcgag gcggtcctcg tgatgggacg gaacctcggg
    16681 gccgactcga tgcagcgctg gctgggcgag cagggcttcc ccaccgagcg cctggcatcg
    16741 aagcgcggct accgcttgct ccggtcgcgg cgcagctaga tccgaagcgg gtcgaccgac
    16801 aggcgcaggc gtcccggcgg gcggtgcacc gcggcgagcg cgtcgcagag ggtctggtgg
    16861 tcgggggccc gcacccgcca cgccccgtcg acgggaccct gcacctgcac gtctggtggg
    16921 gccgacccgg tgagcgcggc gacgaacgcc tcgc
//
LOCUS       DATISU010000434        10052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_132994, whole genome shotgun sequence.
ACCESSION   DATISU010000434 DATISU010000000
VERSION     DATISU010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10052
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132994"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /gene="map"
                     /locus_tag="VLR27_17330"
     CDS             <1..533
                     /gene="map"
                     /locus_tag="VLR27_17330"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HSP05272.1"
                     /translation="ICHGIPDDRELVDGDIVNIDVTIFREGVHGDTNRTFFVGEVDEA
                     SRRLVQVTHESLLRGIEAVKPGQPFSVIGQAIQAHAEGEGFGVVRAFVGHGIGQQFHT
                     DLHIPHYHEPRLTEIIEPGMTFTIEPMITVGAHEHVMWSDGWTAVTIDRKRTAQFEHT
                     ILVTDDGAEVLTPHPD"
     gene            complement(588..1808)
                     /locus_tag="VLR27_17335"
     CDS             complement(588..1808)
                     /locus_tag="VLR27_17335"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HSP05273.1"
                     /translation="MPRPTSLSAAQARRIVLAAQGLASPRPAGRVDRRHMRSVIRRLG
                     LVQMDSVNVLARAHHLPFFSRLGPHDAELLDRIAYRDHELFEYWGHEASLIDVELEPL
                     LRWRMADGHRWSGPASVGQRRPDLLAQLEALVHEHGPVSAAELDAMLGIDRDRTGPWW
                     GWGDTKKALENLFWHGRVGAVRRGGFERAYCHPAVTVPADVRARPTPDPEDALRHLVL
                     VAARAHGVATPKDLADYWRLPIRAVRALVPGLVADGQLEAVAVEGWSEPGLRLPDAAL
                     PRRVDACALVSPFDVAMWHRDRVERIHRFRYVIEIYVPEAKRVHGYYVLPFLLGDTYV
                     ARVDLKADRPGRRLLVQSAWAEPDLDQRGTDVAEVAERLHGELRLLADWLALDDVVVV
                     DRGDLAPALAATTT"
     gene            complement(1846..2550)
                     /locus_tag="VLR27_17340"
     CDS             complement(1846..2550)
                     /locus_tag="VLR27_17340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSP05274.1"
                     /translation="MRDAANVTVELPADEDPDPAPLFGVDLSSLITVAHQAAAGDLVS
                     AVLEGRSVVARWDGVEVFTDGDTTELVGFHESNSTEALPLDSTRPIASSHHHAAAVPT
                     TGETDGQVVVLPTRQRATGPMTAIDLAVDAGAPVTAPVDGVVVQVAPIHYRGSDHKIT
                     IRPHANPDVEVSIIHVVEPLVAAGQHVRGGETPVATGPRQLGFESQVDRFTAAARGAA
                     APHVHVEMRATTPAAA"
     gene            2871..3071
                     /locus_tag="VLR27_17345"
     CDS             2871..3071
                     /locus_tag="VLR27_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HSP05275.1"
                     /translation="MGYKVVVDFDLCESNAICMGIAPEVFEVRDDDFLYVLQEHPSEE
                     LRPKVEEAVQRCPKQAISIEDE"
     gene            3083..4282
                     /locus_tag="VLR27_17350"
     CDS             3083..4282
                     /locus_tag="VLR27_17350"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSP05276.1"
                     /translation="MRDIVIVGASIAGFSAAAELRARGHDGPIHLVGEEPVRTYERPP
                     LSKGVLGGEDPTTTEWAAARDDDLGLDWLVGERAVGLDVAGRSVELATGESLPYDGLV
                     IATGASPRRLPGHRDRAGLHVLRSLDDCLGLRADLDAGPERVVVVGAGFIGGEVAATC
                     RHRGHEVTVVEPLSQPLERVLGPTIGAAVAGVHRAAGVDLRLGTGVDAVLGDDHVEGV
                     RTTDGTVIEADLVVVGIGVVPNTAWLDGSGLPVDDGIRCDATTLVAPGITAAGDVASW
                     DSPRYGRLRIEHWDNAADMGRAAGRRLVVSDDEAEAYDPVPWFWSDQHDVRVQVAGRP
                     TLPDQGGELAVVEGQIGEERFVALYGRDGQVTGALGWNWPAKTVRYRMALEEGLAWDE
                     AIAAASP"
     gene            complement(4289..5314)
                     /locus_tag="VLR27_17355"
     CDS             complement(4289..5314)
                     /locus_tag="VLR27_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05277.1"
                     /translation="MSGTDLEHRTEIRKANRTTPIWIGLVALTALVALVVGRGDDDGE
                     AAPDPGPTTVPATPTSETLTTTSPRGLPPVYGLTPAGGEGALFAFSTATGPSGPSGLA
                     MDQVQAAVTTDDVVVVLAGGRVHAAVPGESFEEVVGCCIEGLHPSNEPGHVWLHDEDE
                     VSLLQIEGGGYVTELDIGDGEVLGPASFGVVVRSSDGAVRWHRPSFEPTDLGGSSDDR
                     RAIDAGGERLLVAAPATATSGARLEVWSITGNGLLSTHELADGFEPEGLLAPDGSVVL
                     VPAPGGWEVRDAATGLLHGTLPKVTGPVWVGGNRFAATLDDQLVVSDSGVLPLRWPVL
                     AVAEQSP"
     gene            5338..5973
                     /locus_tag="VLR27_17360"
     CDS             5338..5973
                     /locus_tag="VLR27_17360"
                     /inference="COORDINATES: protein motif:HMM:NF019559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HSP05278.1"
                     /translation="MDGTVIALLAATLVVAAVDWWAVATDRRRVEYVAKPLTMVVLIA
                     AALALDPVDGTARTWFVIALVLSLAGDVFLMLPRDLFVAGLASFLLGHVAYIVGLLSM
                     DTSVAGLAVGVVIIAAALPWLGVRILRAVRTGSEPALAAPVTAYMLVISAMVISAGAS
                     GSAIALAGAVSFYVSDALIAWTRFIQPLAWGRVAVMVTYHAAQIALVASLV"
     gene            complement(5980..7191)
                     /locus_tag="VLR27_17365"
     CDS             complement(5980..7191)
                     /locus_tag="VLR27_17365"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSP05279.1"
                     /translation="MSIKVHESSRRTRFVNALRSHVPPLPPMDGPPRPPKRLEVFGTG
                     TTWIQLTWSALGPGPVEVRCGDQVIPLDTDGGPGAVTVTELSSGSEHRIHVTGPGLPA
                     PIAPLVARTLLPPPGALLHRIATVSDTHIGSRSTGFLHTIIEKPQPEEAHPVRCARAA
                     LREARAWGADELVVKGDLVDHSQPEWWAQAASVLGELDVPISVVPGNHEWSGRGDRDP
                     ADGLARHGLDLVTDVAHRDRPGVRLVLLDSSIPGEDPGRVSDVLERTLALARDTDLPL
                     LVAMHHQPTRWRIPTYLPAGIPGPEADHFLRALGQANPRTIVTSGHTHRHRRRHVGPV
                     TVTEVGSTKDFPGTWAGYEVHEGGIVQTVHRVAEPSCIRWTDHTRRAALGVWQLWSPG
                     RLGDRCFTRTW"
     gene            complement(7188..9782)
                     /locus_tag="VLR27_17370"
     CDS             complement(7188..9782)
                     /locus_tag="VLR27_17370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HSP05280.1"
                     /translation="MPSDDATATSTDHGREAGRLPTEVRPAHYDLRLAPDLATSTFRG
                     AVAITLDVGAPTDAVVCNAAELDVAEAWVDLDGRHIDGEIRLDADLEQLRVSLPETLP
                     AGPAVLHLVFSGVLNDRLRGFYRSTFTAEDGGDRAVAVTQFEPTDARRAFPCWDEPAF
                     KATFGVTLDVAEDLLAVSNGPELSREDLGEGRVRIRYADTMPMSTYLVAFVVGPMETT
                     GPVDVDGVPLRIVHRPGQGELAEFALEVGAFALRYFADYYGIAYPGDKVDMVAVPDFA
                     FGAMENLGCVVYRETLLLVDPESATQSELLAVVDVIAHELAHMWFGDLVTMRWWNGIW
                     LNEAFATFMEAKCAAAFRPAWERWQHFGRERSGAFDVDALATTRPIEYPVRTPADAEG
                     MFDTLTYQKGSAVVRMLEQFLGEDAFRDGIRRYLQTHQFGNTETGDLWDALEAVTGEP
                     VREMMDSWIFQGGFPLVSAGLSDGTLHLSQQRFRYLEASEPDGGEDARWHVPVVVRSA
                     NGTERVLLDGPDAALPLDVATSPGAPVVVNAGGHAFYRVSYDAELHAAVLDGLEALAP
                     LERYAVLDDAHGLTLRGDRRAADLAHVVQRLADVGETDLAIWQLAASSIDVVHRAASE
                     EERPAVSGWVRRLLAPLAVELGEEVDPTDDDRTRALRGLVLGLRGVHGDPDAGARATA
                     VFDQLLHRPADVDAELGAGALKSAAVTADAARFEAMLDRYRHGATPQEQVRHLYAAAT
                     VADGELFGRYLALLETDQVRSQNLAFAYRAALRNLAHERDAWAAVRDGWERIRTRLPF
                     NANHRLVEGITGITDPDLAGEVEEFLAAHPVPEATTLIAQHVERMRVQVAVHQREAGR
                     VASSLPGG"
     gene            9820..>10052
                     /locus_tag="VLR27_17375"
     CDS             9820..>10052
                     /locus_tag="VLR27_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05281.1"
                     /translation="MTPPASEQILHHVRDDGTALDVVGIGNALVDVLSHEADDFVAAH
                     DLVKGAMTLIEPDRATELYEAMGPGIEVSGGSAA"
BASE COUNT         1323 a         3552 c         3846 g         1331 t
ORIGIN      
        1 tcatctgcca cggcatcccc gacgaccgcg agctggtcga cggcgacatc gtgaacatcg
       61 acgtcaccat cttccgcgag ggcgtccacg gcgacaccaa ccgcaccttc ttcgtcggcg
      121 aggtcgacga ggcctcccgc cgcctggtgc aggtcaccca cgagtcgctc ctccggggca
      181 tcgaggcggt gaagccgggc cagccgttct cggtcatcgg ccaggccatc caggcccacg
      241 cggagggcga ggggttcggc gtcgtgcgcg ccttcgtggg gcacggcatc ggccagcagt
      301 tccacaccga cctgcacatc ccgcactacc acgagccccg cctcaccgag atcatcgagc
      361 cgggcatgac cttcacgatc gagcccatga tcacggtcgg cgcccacgag cacgtgatgt
      421 ggagcgacgg gtggacggcg gtcaccatcg accgcaagcg caccgcgcag ttcgagcaca
      481 cgatcctcgt caccgacgac ggcgccgagg tgctcacccc ccaccccgac tgaggcttcc
      541 caacgcgcac atcgacgccg agcggtcgat ctgcgcgaag ggaacgctca cgtggtggtg
      601 gcggcgaggg cgggggcgag atcgccccgg tcgacgacga ccacgtcgtc gagggcgagc
      661 cagtcggcca gcaggcgcag ctcgccgtgg aggcgctcgg ccacctcggc cacgtcggtg
      721 ccccgctggt ccaggtccgg ttcggcccac gccgactgca cgaggaggcg ccgcccgggg
      781 cggtcggcct tgaggtcgac gcgggcgacg taggtgtcgc cgagcaggaa cggcagcacg
      841 tagtagccgt gcacccgctt ggcctcgggc acgtagatct cgatcacgta ccggaaccgg
      901 tggatgcgct ccacccggtc gcggtgccac atggcgacgt cgaacgggga gaccagcgcg
      961 cacgcgtcga cccggcgggg gagcgcggcg tccgggaggc gcaggcccgg ctccgaccag
     1021 ccctccaccg ccaccgcctc cagctgcccg tcggcgacca ggcccgggac cagggcacgg
     1081 acagctctga tcggcagccg ccagtagtcg gccaggtcct tcggggtggc gacgccgtgg
     1141 gcgcgcgccg cgaccaggac caggtggcgc agcgcatcct ccgggtcggg tgtcgggcgg
     1201 gctcggacgt ctgctggcac cgtcacggca gggtggcagt aggcccgctc gaacccgccg
     1261 cgccgcacgg caccgacccg gccgtgccag aagaggttct cgagcgcctt cttggtgtcg
     1321 ccccagcccc accacggccc ggtgcggtcc cggtcgatgc cgagcatcgc atcgagctcg
     1381 gcggccgaca cggggccgtg ctcgtgcacc agcgcctcca gctgcgcgag caggtccggg
     1441 cgacgctggc ccacgctggc cggtccgctc caccggtgcc cgtcggccat gcgccaccgc
     1501 aggagcggct ccagctccac gtcgatgagc gacgcctcgt gcccccagta ctcgaacagc
     1561 tcgtggtccc ggtaggcgat ccggtcgagc agctcggcgt cgtgcgggcc gaggcggctg
     1621 aagaacggca ggtggtgcgc ccgggccagc acgttcaccg agtccatctg gaccaggccg
     1681 aggcggcgga tgacggaccg catgtggcgc cggtcgacac gaccggctgg ccggggtgag
     1741 gcgagccctt gggcggcgag gacgatgcga cgggcctggg cggcgctgag cgaggtcgga
     1801 cgaggcacgg cacggcagtc tggcccctcc gagcccgctg cggggtcagg cggcggccgg
     1861 ggtggtggct cgcatctcca cgtgcacgtg gggggcggcg gcgccccggg ccgccgcggt
     1921 gaagcggtcg acctggctct cgaacccgag ctggcggggg ccggtggcca cgggcgtctc
     1981 accgccgcgg acgtgctggc cggcggcgac gaggggctcg acgacgtgga tgatcgagac
     2041 ctcgacgtcg gggttggcgt gggggcggat ggtgatcttg tggtcgctgc cgcggtagtg
     2101 gatcggtgcg acctgcacga cgacgccgtc gacgggggcg gtgaccgggg cgccggcgtc
     2161 caccgcgagg tcgatcgccg tcatcgggcc ggtggcccgc tggcgggtgg gcaggacgac
     2221 gacctggccg tcggtctcgc cggtggtcgg gaccgccgcg gcgtggtggt ggctcgacgc
     2281 gatcggccgg gtggagtcga gcggcagcgc ttcggtgctg ttggactcgt ggaagccgac
     2341 gagctcggtg gtgtcgccgt cggtgaacac ctccacgccg tcccaccgcg cgacgacgga
     2401 gcggccctcg agcacggcgc tcacgaggtc accggcggcg gcctggtggg cgacggtgat
     2461 cagcgacgac aggtcgaccc cgaagagcgg ggcggggtcg gggtcctcgt cggcggggag
     2521 ctcgacggtg acgttcgcgg cgtcgcgcac gctcgcactg gtcggcgccg gcgaggtcac
     2581 cacgagaccg gtcagacctg cggccaggag gccctgtcga cggctgcgac gccggcgcgc
     2641 cggaggccct ccagcggacg gtcgggtggg gaacaggtcg tcttcggtca tggcacctct
     2701 cggagcggcg gtcgatccag gccctgcggg ggcaccgacg gtgggcgctc gcgccgaagg
     2761 cctcggtggg tccgtaccgc gctgcggcgg gtccgatgca tctcggggcc ggtcggcagg
     2821 cgcggtacgc tccgccgtct ccggacgccg aacgagaagg ggtacacgag atgggctaca
     2881 aggtggtcgt cgacttcgac ctgtgcgagt cgaacgcgat ctgcatgggc atcgccccgg
     2941 aggtgttcga ggtgcgtgac gacgacttcc tctacgtcct ccaggagcac cccagcgagg
     3001 agctgcgccc caaggtcgaa gaggccgtgc agcgctgccc caagcaggcc atctccatcg
     3061 aggacgagta gcgcacccac cgatgcgcga catcgtcatc gtcggcgcat ccatcgccgg
     3121 cttctcggcc gccgccgagc tgcgcgcccg gggccacgac ggcccgatcc acctcgtggg
     3181 cgaggagccg gtccgcacct acgagcggcc gccgctgtcc aagggcgtgc tgggcggcga
     3241 ggacccgacc accaccgagt gggccgcggc gcgcgacgac gacctgggcc tcgactggct
     3301 ggtgggggag cgcgccgtcg gactcgacgt cgccgggcgg tccgtggagc tggccaccgg
     3361 cgagtcgctg ccctacgacg gcctggtcat cgcgaccggc gcatcgcccc gccgcctgcc
     3421 cggccaccgc gaccgggccg gcctgcacgt gctgcggtcc ctcgacgact gcctcggcct
     3481 ccgcgccgac ctcgacgccg gtcccgagcg cgtcgtggtg gtgggggccg gcttcatcgg
     3541 cggtgaggtc gcagccacgt gccgccaccg gggtcacgag gtcacggtcg tcgaacccct
     3601 gtcccagccc ctcgagcgcg tgctcgggcc caccatcggg gcagcggtcg ccggtgtgca
     3661 ccgggccgcc ggcgtcgacc tgcggctcgg caccggtgtc gacgccgtgc tcggcgacga
     3721 ccacgtcgaa ggggtccgga ccaccgacgg cacggtgatc gaggccgacc tcgtggtcgt
     3781 gggcatcggc gtggtgccca acaccgcctg gctcgacggc tccgggctcc cggtcgatga
     3841 cggcatccgg tgcgatgcca ccaccctcgt ggcgccgggc atcaccgcag ccggcgacgt
     3901 cgcgtcgtgg gacagccccc gctacggccg cctgcgcatc gagcactggg acaacgccgc
     3961 ggacatgggc cgcgctgccg gccgccgcct ggtggtgagc gacgacgagg ccgaggccta
     4021 cgacccggtg ccgtggttct ggtccgacca gcacgacgtc cgggtccagg tggccggccg
     4081 cccgacgctg ccggaccagg gaggcgagct cgccgtggtc gagggacaga tcggcgagga
     4141 gcgcttcgtg gccctctacg gccgggacgg gcaggtcacg ggcgcgctgg gctggaactg
     4201 gccggccaag accgtgcgct accgcatggc gctggaggag ggcctcgcct gggacgaggc
     4261 gatcgccgcg gcgtcgccgt agcggcgttc agggcgactg ctcggccacg gccagcaccg
     4321 gccaccgcaa cggcagcacg ccgctgtcgg acaccacgag ctgatcgtcg agcgtggcgg
     4381 cgaaccggtt cccaccgacc cagacgggcc cggtgacctt cggcagcgtg ccgtgcagca
     4441 ggccggtcgc ggcgtcgcgc acctcccagc cgcccggtgc cggcaccagc accaccgatc
     4501 cgtccggtgc cagcaggccc tcgggctcga agccgtcggc gagctcgtgg gtggagagca
     4561 gcccgttgcc ggtgatcgac cagacctcca gacgggcacc ggacgtggcc gtggccggcg
     4621 cggcgaccag gaggcgctcg cccccggcgt cgatggctcg acggtcgtcg gaggaaccac
     4681 ccagatccgt cggctcgaag ctgggccggt gccagcggac cgcgccgtcg ctgctgcgga
     4741 cgacgacccc gaagctcgcc gggccgagga cctccccgtc gccgatgtcg agctcggtga
     4801 cgtatccacc gccctcgatc tgcaggagcg agacctcgtc ctcgtcgtgc agccacacgt
     4861 ggccgggctc gttggagggg tgcaacccct cgatgcagca gcccaccacc tcctcgaagc
     4921 tctcgcccgg caccgcggcg tggaccctgc cgccggccaa caccaccacg acgtcgtcgg
     4981 tcgtcaccgc ggcctgcacc tggtccatgg cgagaccgct gggtcccgac gggccggtgg
     5041 cggtcgagaa cgcgaagagc gcgccctcgc cccccgccgg cgtcagtccg tacaccggcg
     5101 gcaggcctcg gggtgacgtg gtcgtcagcg tctcgctcgt gggcgtcgcc gggaccgtcg
     5161 tcggtccggg atccggtgcg gcctcgccgt cgtcgtcccc gcggcccacg acgagcgcga
     5221 cgagcgcggt cagcgcgacc agcccgatcc agatcggcgt ggtgcggttc gccttccgga
     5281 tctcggtgcg gtgctcgagg tcggtgccgc tcacaggggg tccagtagac catcgccatg
     5341 gacgggaccg tcatcgcgct cctcgccgcc accctggtcg tggccgccgt cgactggtgg
     5401 gcggtggcaa ccgaccgacg acgcgtcgag tacgtggcca agcccctcac gatggtggtg
     5461 ctgatcgccg cggcgcttgc gctcgacccc gtggacggca ccgcccgcac gtggttcgtg
     5521 atcgccctcg tgctgtccct cgccggcgac gtgttcctga tgctgccccg ggacctgttc
     5581 gtggccggcc tggcgtcgtt cctgctcggc cacgtcgcct acatcgtcgg gctgctgtcc
     5641 atggacacct ccgtggccgg actcgccgtc ggcgtggtca tcatcgccgc ggccctcccg
     5701 tggctcggcg tccggatcct gcgggccgtc cgcaccggca gcgagccggc gctcgccgca
     5761 ccggtcaccg cgtacatgct ggtgatcagc gcgatggtga tctcggcggg ggcgtcgggg
     5821 agcgccatcg cgctggccgg cgccgtgtcg ttctacgtgt ccgacgccct gatcgcctgg
     5881 acccgcttca tccagccgct ggcctggggt cgggtcgccg tcatggtcac ctaccacgcc
     5941 gcccagatcg ccctcgtcgc ctccctggtc tgagcggcgt caccaggtcc gggtgaagca
     6001 gcggtcgccg aggcggccgg gcgaccacag ctgccacacg ccgagcgcgg cgcggcgggt
     6061 gtggtcggtc cagcggatgc aggacggctc ggcgacgcgg tgcacggtct ggacgatccc
     6121 gccctcgtgc acctcgtagc cggcccacgt gcccgggaag tccttggtgg agccgacctc
     6181 ggtgacggtg accggtccca cgtggcgtcg ccggtgccgg tgcgtgtgcc ccgacgtgac
     6241 gatcgtgcgg gggttggcct ggccgagggc gcgcaggaag tggtcggcct ccgggcccgg
     6301 gatgccggcg ggcaggtagg tcgggatgcg ccaccgggtc ggttggtggt gcattgccac
     6361 gaggagcggc aggtccgtgt cccgcgccag cgccagcgtg cgctcgagca cgtcggagac
     6421 ccgaccgggg tcctcgccgg ggatggagct gtcgagcagg acgaggcgga cgccggggcg
     6481 gtcgcggtgg gccacgtcgg tgaccaggtc cagcccgtgg cgggcgaggc cgtctgcggg
     6541 gtcgcggtcg ccgcggccgg accactcgtg gttgccgggc acgaccgaga tcggcacgtc
     6601 gagctcgccg agcaccgagg cggcctgggc ccaccactcg ggctggctgt ggtccacgag
     6661 gtcgcccttg acgacgagct cgtcggcacc ccaggcccgg gcctctcgca gcgcggcccg
     6721 ggcgcagcgc accgggtggg cctcctcggg ctggggcttc tcgatgatcg tgtgcaggaa
     6781 cccggtgctg cgggagccga tgtgggtgtc gctgaccgtg gcgatgcggt ggagcagcgc
     6841 cccggggggc gggagcagcg tgcgtgccac cagcggggcg atcggggcgg gcaggcccgg
     6901 cccggtgacg tggatccggt gctcgctgcc gctcgacagc tcggtgacgg tgacggcacc
     6961 gggaccgcca tcggtgtcga gcgggatcac ctggtccccg cagcgcacct cgaccggtcc
     7021 gggtccgagg gcgctccacg tcagctggat ccaggtggtg ccggtgccga acacctcgag
     7081 ccgcttcggc ggccgtgggg gcccgtccat cgggggcagc ggggggacgt ggctgcggag
     7141 ggcgttgacg aagcgggtgc gtcggctgct ctcgtggacc ttgatgctca tccgccgggc
     7201 agcgacgacg cgacgcggcc ggcctcgcgc tggtgaacgg cgacctgcac ccgcatccgc
     7261 tcgacgtgct gcgcgatgag ggtggtcgcc tccggcaccg ggtgggcggc gaggaactcc
     7321 tcgacctcgc cggcgaggtc cgggtcggtg atgccggtga tgccctcgac caggcggtgg
     7381 ttggcgttga acgggagccg ggtgcggatg cgctcccagc cgtcgcgcac tgcggcccag
     7441 gcatcgcgct cgtgggccag gttgcgcagc gcggcccggt aggcgaacgc caggttctgg
     7501 ctgcggacct ggtcggtctc gaggagcgcc aggtaccgac cgaacagctc gccgtctgcg
     7561 acggtggccg cagcgtagag gtggcggacc tgctcctggg gtgtggcgcc gtggcggtag
     7621 cggtcgagca tggcctcgaa gcgggcggca tccgcagtga ccgcagcgga cttcagcgcg
     7681 ccggcgccga gctcggcgtc gacgtccgcg ggccggtgca ggagctggtc gaacaccgcg
     7741 gtggctcggg cacccgcatc ggggtcgccg tgcacgccgc gcagcccgag cacgagcccg
     7801 cgcagcgcac gggtgcggtc gtcgtcggtg gggtcgacct cctcgcccag ctccacggcc
     7861 agcggcgcca ggagccggcg cacccagccc gacaccgccg gacgctcctc ctcggaggcg
     7921 gcccggtgca ccacatcgat gctcgacgca gccagctgcc agatggcgag gtcggtctcg
     7981 ccgacgtcgg cgaggcgctg cacgacgtgg gcgaggtcag cggcccggcg atcgccccgc
     8041 agggtcaggc cgtgggcgtc gtcgagcacc gcgtagcgct cgagcggggc gagcgcctcg
     8101 aggccgtcga ggacggcggc gtggagctcg gcgtcgtagg acacccggta gaaggcgtgg
     8161 ccgccggcgt tgacgaccac cggagcaccg gggctggtcg cgacgtcgag gggaagggcg
     8221 gcgtcgggac cgtcgagcag gacgcgctcg gtgccgttgg cgctgcggac gacgaccggg
     8281 acgtgccagc gggcgtcctc gccgccgtcg ggttcgctcg cctccaggta gcggaaccgc
     8341 tgctggctga ggtggagcgt gccatcggac agcccggcgg acacgagcgg gaacccgccc
     8401 tggaagatcc agctgtccat catctcgcgc accggctcgc cggtgacggc ctcgagcgcg
     8461 tcccacaggt caccggtctc ggtgttgccg aactggtggg tctgcaggta gcgccggatg
     8521 ccgtcgcgga acgcgtcctc gcccaggaac tgctcgagca tgcggaccac cgccgagccc
     8581 ttctggtacg tgagggtgtc gaacatgccc tcggcatcgg cgggcgtgcg caccgggtac
     8641 tcgatggggc gggtggtggc cagcgcatcg acgtcgaagg cgccggagcg ctcgcggccg
     8701 aagtgctgcc agcgctccca cgccggacgg aaggccgcgg cgcacttggc ctccatgaag
     8761 gtggcgaagg cctcgttcag ccagatgccg ttccaccagc gcatcgtcac caggtcgccg
     8821 aaccacatgt gggcgagctc gtgggcgatc acgtcgacca cggcgaggag ctcggactgg
     8881 gtggccgact cggggtcgac gagcaggagg gtctcccggt agacgacgca tcccaggttc
     8941 tccatggcgc cgaaggcgaa gtcgggcacg gccaccatgt cgaccttgtc gcccggatag
     9001 gcgatgccgt agtagtcggc gaagtaccgg agggcgaagg ccccgacctc gagggcgaac
     9061 tcggccagct cgccctggcc gggccggtgc acgatgcgca gcggcacgcc gtcgacgtcg
     9121 acggggccgg tggtctccat cggcccgacg acgaaggcga ccaggtaggt cgacatcggc
     9181 atcgtgtcgg cgtagcggat gcgcacccgg ccctcgccga ggtcctcccg ggacagctcg
     9241 gggccgttgg acaccgccag caggtcctcg gccacgtcga gggtgacgcc gaaggtggcc
     9301 ttgaaggcgg gctcgtccca gcacgggaag gcgcgacgcg cgtcggtcgg ttcgaactgc
     9361 gtcacggcga cggcgcggtc gccgccgtcc tcggcggtga aggtcgaccg gtagaagccc
     9421 cggaggcggt cgttcagcac gccggagaag acgaggtgca gcacggccgg gccggcgggc
     9481 agggtctccg gcagggagac gcggagctgc tcgaggtcgg cgtcgaggcg gatctcgccg
     9541 tcgatgtggc gcccgtcgag gtcgacccat gcctcggcga cgtccagctc ggcggcgttg
     9601 cacaccaccg cgtcggtggg tgcgccgacg tcgagggtga tggcgaccgc accccggaag
     9661 gtgctggtgg cgaggtccgg agcgagccgc aggtcgtagt gggcaggccg gacctcggtc
     9721 ggcaggcgac cggcctcccg gccgtggtcg gtgctcgtcg cagtggcgtc gtcggagggc
     9781 accgggcaac ggtaccggcc gcgtgtaccg tcgccgagcg tgacgccccc tgcatccgag
     9841 cagatcctcc accacgtccg cgacgacggc accgccctcg acgtggtggg catcggcaac
     9901 gccctggtcg acgtgctcag ccacgaagcc gacgacttcg tggccgccca cgacctggtc
     9961 aagggggcga tgacgctcat cgagcccgac cgggccaccg agctgtacga ggccatgggg
    10021 ccgggcatcg aggtgtcggg gggctcggcg gc
//
LOCUS       DATISU010000435         1800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_365918, whole genome shotgun sequence.
ACCESSION   DATISU010000435 DATISU010000000
VERSION     DATISU010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365918"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..1220
                     /locus_tag="VLR27_17380"
     CDS             393..1220
                     /locus_tag="VLR27_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05282.1"
                     /translation="MNTAQKRTFKGVGTAIVVAGLAATGVAGPAFATRGHTPVTICHA
                     TSSETNPYRPITVDEDSTLYKGHLGHADDLIPAPAGGCPGPVEPPVVVPPVVVPPVVT
                     PPVVVPPIEPPVVTPPVVDPPVVVPPIEEPPVVVPPIEEPPVVVPPIDVLPVPPVEVT
                     PPVVVPPVEEPPAVVPPVEEPPAVVPPVVTLPAPPVVATPVVPTPVVPTPVAATPVAA
                     TPVAAAPVAAAAVAAAPVAETEVLARTGVASWTIPASILAAVMMLAGAALTVRRRRA"
     gene            complement(1275..>1800)
                     /locus_tag="VLR27_17385"
     CDS             complement(1275..>1800)
                     /locus_tag="VLR27_17385"
                     /inference="COORDINATES: protein motif:HMM:NF020129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HSP05283.1"
                     /translation="NGADIGEITGDVFPLSRRLRQHLRSTELWNTLVSEKLGLGSDAM
                     RDLGNLYTAALPAWLASGLKHALSEGLELAGQEWLALGYGSGDAAEALPMRVAAGWQD
                     AAARINFETALQGPVDLTEAQYIALHEGRPGDLVPAPAGDQFVVDHLGTANGPDFYDQ
                     GIEYYRYVAPVAEA"
BASE COUNT          254 a          670 c          605 g          271 t
ORIGIN      
        1 tatttattga ccggggccgt gcgcgcggag gccggaaggg cgccggccgc gaccacgccc
       61 gaagcggcga ggacgacggc gatcacgctg gacaggaggc gggttcgagg cttcatggcc
      121 cgactgtact cgcgcccatc ggcccttggg aaggggaact tcagcggagt cccccggtcc
      181 ccgccgcctc gtgtgtccca gacgcgcgcc ctgcacgcgg gggtccgaat gtgccccgaa
      241 ttggggggct cacgtgcccc actctggggg caacggaatc ggacttttcc ggctggcgtg
      301 tcctcataat gggggacaga catggatcgg ctaccctccc tcgctttggc gaggagttcg
      361 atccattgac acccgatgaa agctggatag caatgaatac tgcccaaaaa aggaccttca
      421 agggggtcgg gaccgccatt gtcgtcgccg gcctcgccgc gaccggtgtc gccggacccg
      481 cgttcgcgac acgggggcac actcccgtca ccatctgcca cgccaccagc agtgaaacga
      541 acccgtacag accgatcacc gtggacgagg actcaaccct gtacaaggga cacctgggcc
      601 acgcggacga cctgatcccc gcgcccgccg gcggctgccc cggcccggtc gagccgcccg
      661 tcgtcgtccc gcccgtcgtc gtcccgcccg tcgtgacgcc tcccgtcgtg gtccccccga
      721 tcgagccccc cgtggtgacg cccccggtcg tggatccgcc cgtcgttgtc ccgcccatcg
      781 aagagccacc cgtcgtcgtt ccgcccatcg aagagccgcc cgtcgtggtc ccgcccatcg
      841 acgtcctgcc cgtcccaccc gtcgaggtga ccccgcccgt cgttgtcccg ccagtcgaag
      901 agccgcccgc cgtcgtccca cccgtcgaag agccgcccgc cgtcgtccca cccgtcgtta
      961 ccctgcctgc cccgccagtc gtagcgacgc cggtcgtccc gacgccggtc gtcccgacgc
     1021 cggtcgcagc gacgccggtc gcagcgacgc cggtcgcagc ggcgccggtc gcagcggcag
     1081 ccgtcgcagc cgcgccggtc gccgaaaccg aggtgcttgc ccgcaccgga gtagcgtcct
     1141 ggaccattcc cgcatcgatc ctggctgcgg tcatgatgct tgccggcgcg gccctgaccg
     1201 tcaggcgccg acgcgcctga cgcggtgaat aggaaacggc ccggacttca cagtccgggc
     1261 cgtttcctgt tgcctcaggc ctcggcgacc ggtgcgacgt agcggtagta ctcgatgccc
     1321 tggtcgtaga agtcggggcc gttcgctgtg ccgaggtggt ccacgacgaa ctggtcgccg
     1381 gccggggccg gcaccagatc gcccgggcgt ccctcgtgca gtgcgatgta ctgcgcctcg
     1441 gtcagatcga ccgggccctg cagcgcggtc tcgaagttga tgcgcgcggc ggcgtcctgc
     1501 cagcccgccg cgacccgcat cgggagagcc tcggcggcgt cgccgctgcc atagccgagc
     1561 gcgagccatt cctggccggc aagctcgagg ccctccgaca gggcgtgctt gagaccggat
     1621 gcgagccacg ccggcagcgc cgccgtgtag aggttgccga ggtcgcgcat ggcgtcggag
     1681 ccgaggccga gcttctcgct gaccagggtg ttccagagct cggtgctgcg caggtgctgc
     1741 cgcaggcggc gggacagcgg gaagacgtcg ccggtgatct cgccgatgtc ggccccgttg
//
LOCUS       DATISU010000436         9957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_692664, whole genome shotgun sequence.
ACCESSION   DATISU010000436 DATISU010000000
VERSION     DATISU010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692664"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VLR27_17390"
     CDS             <1..988
                     /locus_tag="VLR27_17390"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HSP05284.1"
                     /translation="QVRMILIDPKRVEMGQYEGLPHLLTQVVTNPKKAANALSWAVKE
                     MERRYDLLSEVGVRDITGYNEAYDRGDLQEVEDAYQGRLVGSEHAPDAPGPERYERLP
                     FIIVVVDELADLMMVAARDVEDSICRIAQMARAVGIHLIIATQRPSVNVITGVIKANV
                     PARLAFAVSSATDSRVILDQVGAERLVGKGDMLFLGPTSSTPARIQGSWVTEEEVRSI
                     AAHWKGQAPQVSYVEDVQGDEGGDPGAMLPGGGTGDDDDDDLALQAMELIVRSQLGST
                     SMLQRKLKVGFARAGRLMDILEQRGVVGPSIGSKARDVLMTEAELDDLLSKS"
     gene            complement(1023..1643)
                     /locus_tag="VLR27_17395"
     CDS             complement(1023..1643)
                     /locus_tag="VLR27_17395"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP05285.1"
                     /translation="MRSAFARRYDKLTARMEDAWLREARADLLAGLSGTVVELGAGTG
                     RNLVHYPDTVERLILTEPTAAMRDQLRARVNAAAHPFDVEVVDATADRLPFNDGIADA
                     VVSTLVLCSVPALGPVVAEARRVLRPGGELHLIEHVAAARNWAHRIQTRLDPVWNWIE
                     GSCHLDHETRTALAAGGFDVSGVSESVQRGMPPLFHRIARGIAVAP"
     gene            complement(1660..2652)
                     /locus_tag="VLR27_17400"
     CDS             complement(1660..2652)
                     /locus_tag="VLR27_17400"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HSP05286.1"
                     /translation="MAIPSRSHAIRFDRTGGPDVLEWTEVDVDEPGPGQVLVRHSAVG
                     LNFVDTYFRTGLYPARLPSGLGMEAAGVVEAVGEDVDLAVGDRVAYGTGPIGAYAEHV
                     TVPAGGLVRVPDEVDDQTAAAIMLKGLTAQYLLRQTYDVGPGTVVLGHAAAGGVGLIL
                     GQWAKALGATVIGTASTPDKRELALENGYDHVIAYRDEGVDIAASVRELTGGRGVDVV
                     YDGVGADTFMASMDSLRPRGMLVTYGNASGPVPAFEPSILATKGSLFVTRPTLASYAD
                     SPAATQAMADDLFAVVASGDVSIRIGQTFPLADAARAHVELEAGRTTGSTVLLP"
     gene            2651..3619
                     /locus_tag="VLR27_17405"
     CDS             2651..3619
                     /locus_tag="VLR27_17405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HSP05287.1"
                     /translation="MAAIMAAVLLALLAVVVGLAILTKAADVFVSGAARLSAALKVPP
                     VIIGAAVIGFGTSAPEMVVSAIAASQGKLDLAVGNVIGSNVANLSLVLGVASLLCVMS
                     VSSSVLRKEAPLSTAAVVLFAVLVQGGLERWEGAALLVALIAALVWLFTGNAGGEDSE
                     LLGEVEEFLHEEDPPSARHESLRAFIGLVGVIVGAQLAVTGASTIADELGLGEGFVAI
                     TLVAVGTSLPELMTAIAAARKGEDELIVGNLLGSNLFNSLAVGAIAGLVGPGLLDDDK
                     LASIGVGAMLLIAGAGWLFMATRRKVERWEGIVLLGAYAGTLPLLA"
     gene            complement(3620..4144)
                     /locus_tag="VLR27_17410"
     CDS             complement(3620..4144)
                     /locus_tag="VLR27_17410"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSP05288.1"
                     /translation="MPRLTTEQITQFLTEPGHLTRIATVDADGAPSVAPIWFLYDDGR
                     IWFTPREKSSWWHHIQREPRVAMTIDEDEAPFRKVFVKGTVTVEHPPGEDDAWRSRYR
                     DIATRYTPDAFADAYINATVHEPRALLSLPCDPAAVTSWRMPLAGEDPAEVWAPQYYH
                     RRPTPEEWVPERRV"
     gene            4199..5146
                     /locus_tag="VLR27_17415"
     CDS             4199..5146
                     /locus_tag="VLR27_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HSP05289.1"
                     /translation="MTADVTTIATDLQFPEGPIAMDDGSILIVEIKRGTLTWLEADGE
                     VRRRFDLGGGPNGAAIGPDGKVYVCNNGGCFHWEDVMGLTIPGHCPPSWSGGSIQRVD
                     LETGDVETLYTESTAEDGSTVPLRAPNDIVFDAHGGFWFSDHGVRLERTSDRTGLHYA
                     SPDGSSCREVVFPVDAPNGIGLSPDGATLYAAETHTGRVFHWPIGEPGTIDQAQANPA
                     GPGGGHLLAGLPGMQFLDSLAVDGEGWVCVATLINGGITSISPDGETVEHLAFEDPLV
                     TNICFGGDDLRTAYVTLSGTGQLVKTTWPRPGMRLAHQA"
     gene            5194..6519
                     /gene="rimO"
                     /locus_tag="VLR27_17420"
     CDS             5194..6519
                     /gene="rimO"
                     /locus_tag="VLR27_17420"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HSP05290.1"
                     /translation="MAQRFWVETLGCPKNSVDSDKVTGRLLADGYLPADAPDHADLVV
                     VNTCAFIEEARQESIDTILSLSDGRRDGAEVVVTGCMAERYGAELADALPEVDRVVGF
                     GGPVTATETGWVPVSIGGIGRAAAEPAVPALDLLNMPRPAASAPWAYVKVAEGCDRKC
                     GFCAIPSFRGPQRSRQRTDVVAEAESLAASGVREIVLVAQDLASYGRDQGQGARELVP
                     LLDDLSAVVDRLRLLYLYPSELTDELIDAICRTGVPYFDLSLQHVSRPLVRRMRRWGE
                     GSRYLERIHAIREREPDAAFRSNFIVGYPGETEADHDALLAFVEEAQLDWCGFFRYSP
                     EDGTYAADLDGEVPDALVADRLFELRDLQDDITGARRDALVGRTVSVLVDEPGVGRSH
                     REAPEIDGIITVPEHLAVGSIVDVEVVAAEGPEVHAQLPTVGASSERSA"
     gene            6516..7097
                     /locus_tag="VLR27_17425"
     CDS             6516..7097
                     /locus_tag="VLR27_17425"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSP05291.1"
                     /translation="MSDAADRMRYGASALATPANAITIARMALAIPFLAFVANGGSSY
                     WALAAWTVLAFSDGLDGHLARKHGTTRSGAFLDPLADKVLVLGAAWALVVSGTFWWVP
                     VLLITVREIGISVFRSWYARRGIAVPARYSAKVKTVVQELAIAFALLPPIAEHAEWVH
                     VSILWAAVVLTIGTGISYVTDGHRSFSHTGDRG"
     gene            7099..8352
                     /locus_tag="VLR27_17430"
     CDS             7099..8352
                     /locus_tag="VLR27_17430"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HSP05292.1"
                     /translation="MRCEVVAIGTELLLGTVVDSNSSWIGSQLALAGIDCLRSVRVGD
                     NHARMVACLRESLERADAVICCGGLGPTQDDVTREAIAEVMGVELVRDPAVEDVIREM
                     FAARGRRMAENNLRQADVPVGATVIPQTRGTAPGLICPVGEDKVVYAVPGVPHEMQEM
                     VLRAVVPDLRLRSGDTSTIVSRTLRTWGESESSLAELLDGRIQAIDEAHAAGTAAPTL
                     AFNASGIEGIRVRITARGDDEAGALALVDAEDEAVRAVLGDLVFGVDGETMEHAVGAL
                     LVQRGLTLGLAESVTGGLVAARIVSVPGASAWFRGSVVAYASEVKFDVLGVPEGPVVS
                     EEAAAALASGARRVLGADVGLGVTGVAGPDTQDGHEVGTVFLASDLEGDVEVRRIQLP
                     GDRERVRQFATISLLDGLRRRLLAG"
     gene            complement(8349..8900)
                     /locus_tag="VLR27_17435"
     CDS             complement(8349..8900)
                     /locus_tag="VLR27_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05293.1"
                     /translation="MTLTERIVALHAALDAAGLPHAFGGALALAFCTAEPRGTQDIDV
                     NVFLSPREVDRLLDALPEGIAVRDPDRELLARDAQARLWWDETPVDVFLSNHPFHDHA
                     EADRRHVAFAGIERLPVLACQDLAVFKSFFARPKDVVDVATMVALGSVDLGALERSVG
                     ALLGDREQRARFFDRVAEAVPEI"
     gene            complement(8897..9238)
                     /locus_tag="VLR27_17440"
     CDS             complement(8897..9238)
                     /locus_tag="VLR27_17440"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSP05294.1"
                     /translation="MDVATFLRSCRAEAGLTQRQLAERAGTSAAAVCHYERGTRIPRA
                     DTLVRLVAATGATLTWTARRFPADPATLDAMEDNGEALAAVLDLAEHLPRRPRPEAIE
                     APVFAELATAP"
     gene            9485..>9957
                     /locus_tag="VLR27_17445"
     CDS             9485..>9957
                     /locus_tag="VLR27_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HSP05295.1"
                     /translation="MALGNIEKQFGKGAVMKMGEKGSMNIESIPTGALALDLALGIGG
                     LPRGRVTEIYGPESSGKSTLAMHVVAEAQRNGGICAYIDAEHAMDPVYARAIGVDVDE
                     LLISQPDTGEQALEICDMLVRSAAIDVIVVDSVAALTPRAEIEGEMGDTHVGLQAR"
BASE COUNT         1346 a         3653 c         3560 g         1398 t
ORIGIN      
        1 ccaggtgcgc atgatcctca tcgaccccaa gcgggtcgag atgggccagt acgagggcct
       61 gccccacctg ctcacccagg tcgtgaccaa cccgaagaag gcggccaacg cgctgtcgtg
      121 ggcggtcaag gagatggagc ggcgctacga cctgctgtcc gaggtcggcg tgcgcgacat
      181 caccggctac aacgaggcct acgaccgcgg cgacctgcag gaggtcgagg acgcgtacca
      241 gggtcgcctc gtcggctccg agcacgcccc cgacgcgccc ggccccgagc gctacgagcg
      301 gctcccgttc atcatcgtcg tggtcgacga gctcgccgac ctcatgatgg tcgccgcccg
      361 cgacgtcgag gactcgatct gccgcatcgc ccagatggcc cgtgccgtgg gcatccacct
      421 catcatcgcc acgcagcgcc cgtcggtgaa cgtcatcacc ggcgtcatca aggccaacgt
      481 ccccgcccgc ctcgccttcg ccgtgtccag cgccaccgac agccgggtga tcctcgacca
      541 ggtcggcgcc gagcgactgg tcggcaaggg cgacatgctc ttcctcggcc cgacgtcgtc
      601 cacgccggcc cgcatccagg gctcgtgggt caccgaggag gaggtgcgct cgatcgccgc
      661 gcactggaag ggccaggccc cccaggtcag ctacgtcgag gacgtccagg gcgacgaggg
      721 cggcgacccc ggggcgatgc tgcccggcgg cggcaccggc gacgacgatg acgacgacct
      781 cgccctccag gccatggagc tcatcgtgcg cagccagctc ggctcgacct cgatgctcca
      841 gcgcaagctc aaggtcggct tcgcccgggc cggccggctc atggacatcc tcgagcagcg
      901 cggcgtggtc ggaccctcca tcggctccaa ggcccgcgac gtgctgatga ccgaggccga
      961 gctggacgac ctgctctcca agagctgacc ctcgaactgg cagtcgtggt gttgcgcccg
     1021 gctcaggggg cgacggcgat gccgcgggcg atgcggtgga acagcggcgg catgccccgc
     1081 tgcacggact cggacacgcc agagacgtcg aagccgccgg cggcgagcgc ggtgcgggtc
     1141 tcgtggtcga ggtggcagga gccctcgatc cagttccaca ccgggtcgag gcgggtctgg
     1201 atgcggtgcg cccagttgcg cgccgcggcc acgtgctcga tgaggtgcag ctcgccgccc
     1261 gggcgcagca cccggcgggc ctcggccacc acgggcccga gggccggcac cgagcagagg
     1321 accagcgtgg acacgacggc gtcggcgatg ccgtcgttga agggcagccg gtcggcggtg
     1381 gcgtcgacca cctcgacgtc gaagggatgc gccgcggcgt tgacgcgggc acggagctgg
     1441 tcgcgcatgg ctgcggtggg ctcggtgagg atgagccgct cgacggtgtc ggggtagtgc
     1501 accaggttgc gtccggtgcc ggcgccgagc tccacgacgg tgcccgacag cccggccagg
     1561 aggtcggcgc gggcttcacg cagccacgca tcctccatgc gggccgtgag cttgtcgtag
     1621 cggcgggcga aggcggagcg catcaggggc gcaccgtact cagggcagca ggacggtgga
     1681 gccggtggtg cggccggcct ccagctcgac gtgggcccgg gcggcgtcgg ccagcgggaa
     1741 ggtctggccg atgcggatcg acacgtcgcc cgatgcgacg accgcgaaca ggtcatcggc
     1801 catcgcctgc gtggcggcgg gagagtcggc gtagctggcc agggtgggcc gggtgacgaa
     1861 cagcgagccc ttcgtggcca ggatcgacgg ctcgaaggcc ggtaccggcc cggatgcgtt
     1921 gccgtaggtg accagcatcc cccgggggcg cagcgagtcc atcgacgcca tgaacgtgtc
     1981 ggccccgacg ccgtcgtaga cgacgtccac gccgcggccg ccggtgagct cccgcaccga
     2041 tgcggcgatg tcgacgccct cgtcccggta ggcgatcacg tggtcgtagc cgttctccag
     2101 cgccagctcg cgcttgtccg gcgtgctggc cgtgccgatc accgtggcgc cgagcgcctt
     2161 tgcccactgg ccgaggatca gcccgacccc gccggccgcg gcgtggccga gcaccaccgt
     2221 gccgggcccg acgtcgtagg tctggcgcag caggtactgg gcggtgaggc ccttgagcat
     2281 gatcgctgca gccgtctggt cgtcgacttc gtcgggcacc cgcacgaggc cgccggcagg
     2341 caccgtgacg tgctcggcgt aggcgccgat gggcccggtg ccgtacgcga cccggtcgcc
     2401 gacggcgagg tcgacgtcct cgccgaccgc ttcgaccacg ccggcggcct ccatgccgag
     2461 cccgctcggc aggcgagccg ggtagagccc ggtgcggaag taggtgtcca cgaagttgag
     2521 cccgacggcg ctgtggcgga cgagcacctg gccgggaccg ggctcgtcga catcgacctc
     2581 ggtccactcg agcacgtcgg gtccaccggt gcggtcgaag cggatggcgt gggatcggga
     2641 ggggatggcc atggctgcaa tcatggccgc cgtgctgctc gccctcctcg ccgtcgtggt
     2701 cggcctggcc atcctgacca aggcggccga cgtgttcgtc agcggcgccg cccggctgtc
     2761 ggctgcgctc aaggtgccgc cggtgatcat cggcgccgcc gtcatcggct tcggcacgag
     2821 cgcgccggag atggtggtgt ccgccatcgc cgccagccag ggcaagctcg acctcgccgt
     2881 cggcaacgtc atcggctcca acgtggccaa cctgtcgctg gtcctcgggg tcgcctccct
     2941 gctgtgcgtg atgagcgtgt cctccagcgt cctgcgaaag gaggcgccgc tctcgaccgc
     3001 cgctgtggtc ctcttcgccg tgctcgtcca gggcgggctc gagcggtggg agggcgccgc
     3061 cctgctcgtg gcgctgatcg ctgcgctggt ctggctgttc accggcaacg ccggcggcga
     3121 ggactccgag ctgctcggcg aggtcgagga gttcctgcac gaggaggacc cgccgtcggc
     3181 ccgccacgag tccctgcgcg ccttcatcgg gctcgtcggc gtgatcgtcg gcgcgcagct
     3241 ggccgtcacc ggggcgtcga ccatcgccga cgagctcggc ctgggcgagg gcttcgtcgc
     3301 catcaccctc gtcgccgtcg gcacgtcgct gcccgagctc atgaccgcca tcgccgcggc
     3361 gcgcaagggc gaggacgagc tcatcgtcgg caacctgctc ggctccaacc tgttcaacag
     3421 cctcgccgtc ggggccatcg ccggactggt cgggcccggg ctgctcgacg acgacaagct
     3481 ggccagcatc ggcgtcggcg ccatgctgct catcgccggc gccggatggc tcttcatggc
     3541 cacccgccgg aaggtggagc ggtgggaggg catcgtgctg ctcggcgcct acgccggcac
     3601 gctgcccctg ctggcgtagt cagacgcgcc gctccggcac ccactcctcg ggcgtcgggc
     3661 gccggtggta gtactgcggc gcccacacct cggcggggtc ctcgccggcg agcggcatgc
     3721 gccacgacgt caccgctgcg gggtcgcacg gcagcgacag cagcgctcgg ggttcgtgca
     3781 ccgtggcgtt gatgtaggca tccgcgaacg catccggcgt gtagcgcgtg gcgatgtcgc
     3841 ggtagcggga gcgccaggcg tcgtcctcgc cgggcgggtg ctcgacggtg accgtgccct
     3901 tgacgaacac cttgcggaac ggcgcctcgt cctcgtcgat ggtcatggcc acgcgcggct
     3961 cgcgctggat gtggtgccac cacgacgact tctcgcgggg cgtgaaccag atgcggccgt
     4021 cgtcgtacag gaaccagatc ggcgccaccg acggcgcgcc gtcggcatcg acggtggcga
     4081 tgcgggtgag gtggcccggc tcggtcagga actgggtgat ctgctccgtg gtcagtcgcg
     4141 gcatgtcgga acgctacgtg gaaacaccgg ttgcctgtcg cgtgccaaga tccgggccat
     4201 gaccgccgac gtcaccacca tcgccaccga cctccagttc cccgagggcc ccatcgccat
     4261 ggacgacggg tcgatcctga tcgtggagat caagcggggc acgctgacct ggctcgaggc
     4321 cgacggcgag gtccgtcgcc gcttcgacct cggcggcggc cccaacggtg ccgccatcgg
     4381 acccgacggc aaggtctacg tgtgcaacaa cggcgggtgc ttccactggg aggacgtcat
     4441 gggcctgacg atcccggggc actgcccacc cagctggagc ggcgggtcca tccagcgggt
     4501 cgacctggag accggtgacg tcgagacgct ctacaccgag tcgacggccg aagacggctc
     4561 caccgtcccc ctccgagccc cgaacgacat cgtcttcgac gcccacggcg ggttctggtt
     4621 cagcgatcac ggcgtccgcc tcgagcgcac ctccgaccgc accgggctgc actacgcgtc
     4681 gccggacggc tcctcgtgcc gcgaggtcgt gttcccggtc gacgcgccga acggcatcgg
     4741 cctctcaccc gacggcgcca ccctctacgc cgccgagacc cacaccggtc gcgtcttcca
     4801 ctggccgatc ggcgaacccg gcacgatcga ccaggcgcag gcgaacccgg caggtcccgg
     4861 cgggggccac ctgctcgccg gcctgcccgg catgcagttc ctcgactcgc tcgccgtcga
     4921 cggcgagggc tgggtctgcg tggccacgct catcaacggc ggcatcacct cgatctcgcc
     4981 cgacggggag accgtggagc acctggcgtt cgaggacccg ctcgtcacca acatctgctt
     5041 cgggggcgac gacctgcgca cggcctacgt cacgctctcc ggcaccggcc agctcgtgaa
     5101 gacgacgtgg ccgcggcccg gcatgcgcct ggcgcaccag gcctgagcgg ggcgactcgg
     5161 gagccggccg ggtaccccgg taccctcacc ccgatggcgc agcgcttctg ggtcgagacg
     5221 ctggggtgcc cgaagaactc ggtcgactcc gacaaggtca ccggtcggct gctcgccgac
     5281 gggtacctcc cggctgacgc gcccgaccac gccgacctcg tcgtggtgaa cacctgcgcg
     5341 ttcatcgagg aggcccgcca ggagtcgatc gacacgatcc tctccctcag cgacgggcgc
     5401 cgcgacggcg ccgaggtcgt cgtcaccggg tgcatggccg agcgctacgg cgccgagctc
     5461 gccgatgcgc tgcccgaggt cgaccgcgtc gtcggcttcg gcggtccggt caccgccacc
     5521 gagaccggct gggtgccggt cagcatcggc ggcatcggcc gagctgcggc cgagccggcc
     5581 gtacccgccc tcgacctgct caacatgccc cgcccggccg cctcggcgcc gtgggcctac
     5641 gtgaaggtgg ccgagggctg cgaccgcaag tgcggcttct gcgccatccc ctccttccgc
     5701 ggcccgcagc gctcacggca gcgcaccgac gtggtcgccg aggccgagtc gctggccgcc
     5761 tccggcgtcc gcgagatcgt gctcgtcgcc caggacctgg cctcctacgg acgggaccag
     5821 ggccaggggg ccagggagct ggtgccgctg ctcgacgacc tgtccgccgt cgtcgaccgc
     5881 ctccgcctgc tctacctcta tccctccgag ctcacggacg agctgatcga cgccatctgc
     5941 cgcaccggtg tcccgtactt cgacctgtcg ctccagcacg tgtcccgtcc gctggtgcgg
     6001 cgcatgcggc gctggggcga gggctcccgc tacctcgagc gcatccacgc catccgcgag
     6061 cgcgagcccg acgccgcgtt ccgctccaac ttcatcgtcg gctacccggg cgagaccgag
     6121 gccgaccacg acgcgctcct cgccttcgtc gaggaggccc agctcgactg gtgcgggttc
     6181 ttccgctact cgcccgagga cggcacctac gccgccgacc tcgacggcga ggtccccgac
     6241 gccctcgtcg ccgatcgcct cttcgagctc cgcgacctgc aggacgacat caccggcgcc
     6301 cggcgcgacg ccctggtcgg gcgcaccgtg tccgtgctcg tcgacgagcc cggggtcggt
     6361 cgcagccacc gcgaggcccc ggagatcgac ggcatcatca ccgtgcccga gcacctcgcc
     6421 gtcggctcca tcgtcgacgt cgaggtcgtc gccgccgagg ggcccgaggt ccacgcccag
     6481 ctgcccacgg tcggagcgag ctcggagcgc agcgcgtgag cgacgccgcc gaccgcatgc
     6541 gctacggcgc atccgccctg gccacgccgg ccaacgccat caccatcgcc cggatggcgc
     6601 tggccatccc gttcctcgcc ttcgtggcca acggcggctc gtcgtactgg gcgctcgcgg
     6661 cctggacggt gctggccttc agcgacggcc tcgacggcca cctggcccgc aagcacggca
     6721 cgacccgctc cggtgcgttc ctcgaccccc tcgccgacaa ggtgctggtg ctcggcgccg
     6781 cgtgggccct cgtggtctcg ggcacgttct ggtgggtgcc ggtgctgctc atcacggtgc
     6841 gcgagatcgg catctcggtc ttccgctcct ggtacgcccg ccggggcatc gccgtcccgg
     6901 cccggtactc ggccaaggtg aagacggtgg tccaggagct ggccatcgcc ttcgcgctgc
     6961 tgcccccgat cgccgagcac gccgagtggg tgcacgtgtc gatcctctgg gcagcggtgg
     7021 tgctgaccat cggcaccggc atctcctacg tcaccgacgg ccaccgctcc ttcagccaca
     7081 ccggcgaccg cggctgacgt gcgctgcgag gtcgtcgcca tcggcaccga gctcctgctc
     7141 ggcacggtcg tggactccaa ctcgtcctgg atcgggtccc agctcgccct cgccgggatc
     7201 gactgcctgc gctcggtccg ggtcggtgac aaccacgcac gcatggtcgc ctgcctgcgg
     7261 gagtccctgg agcgggccga cgccgtgatc tgctgcggcg ggctcgggcc cacccaggac
     7321 gacgtgaccc gggaggccat cgccgaggtc atgggcgtcg agctcgtgcg ggacccggcc
     7381 gtcgaggacg tgatccgcga gatgttcgcc gcccgcggcc ggcgcatggc cgagaacaat
     7441 ctgcgccagg ccgacgtgcc cgtcggtgcg accgtcatcc cccagacgcg gggcacggca
     7501 ccgggcctga tctgcccggt gggcgaggac aaggtcgtct acgcggtgcc gggcgtgccc
     7561 cacgagatgc aggagatggt gctgcgggcc gtcgtgcccg acctgcgcct gcgctccggc
     7621 gacacgtcca ccatcgtgtc ccggaccctc cggacctggg gcgagtccga gtcctcgctg
     7681 gccgagctgc tcgacgggcg catccaggcc atcgacgagg cccacgccgc cggcaccgcg
     7741 gccccgacgc tggcgttcaa cgcctcgggc atcgagggca tccgggtgcg catcaccgcc
     7801 cgcggcgacg acgaggcggg tgcgctcgcc ctcgtcgacg ccgaggacga ggcggtccgc
     7861 gccgtgctcg gcgacctggt gttcggggtc gacggcgaga cgatggagca cgccgtcggt
     7921 gcgctgctgg tccagcgtgg cctcaccctc ggcctggccg aatcggtcac cggtggcctg
     7981 gtcgcggccc gcatcgtgtc ggtgccgggt gccagtgcgt ggttccgcgg ctccgtcgtg
     8041 gcctacgcgt ccgaggtgaa gttcgacgtg ctcggggtgc ccgagggacc ggtcgtgtcc
     8101 gaggaggccg cggccgccct ggcctcggga gcccgacggg tcctcggcgc cgacgtgggc
     8161 ctcggcgtca ccggcgttgc cggacctgac acccaggacg gccacgaggt cggcaccgtg
     8221 ttcctcgcca gcgacctcga gggcgacgtc gaggtgcgcc gcatccagct gcccggcgac
     8281 cgggagcggg tgcgtcagtt cgccaccatc tcgctgctcg acgggctgcg ccggcgtctg
     8341 ctcgccggct agatctccgg cacggcttcg gccacccgat cgaagaaccg ggcccgctgc
     8401 tcgcggtccc cgagcagagc gccgaccgac cgctcgagcg ccccgaggtc gaccgaaccg
     8461 agggccacca tggtcgccac gtcgacgacg tccttgggcc gggcgaagaa cgacttgaac
     8521 acggcgaggt cctggcaggc gagcaccggg aggcgctcga tcccggcgaa ggcgacgtgg
     8581 cgccggtcgg cctcggcgtg gtcgtggaac gggtggttgg acaggaacac gtcgaccggc
     8641 gtctcgtccc accacagccg ggcctgggcg tcgcgcgcca gcagctcccg gtcggggtcg
     8701 cgcacggcga tgccctcggg cagggcgtcg agcaggcggt cgacctcccg gggcgacagg
     8761 aacacgttga cgtcgatgtc ctgggtgccc cgcggctcgg cggtgcagaa cgcgagggcg
     8821 agcgcgccgc cgaaggcgtg ggggaggccg gccgcgtcga gcgccgcgtg cagcgccacg
     8881 atgcgctcgg tgagcgtcac ggtgccgtgg cgagctcggc gaacaccggc gcctcgatgg
     8941 cctcgggccg gggccggcgg gggaggtgct cggccaggtc gaggacggcg gccaaggcct
     9001 cgccgttgtc ctccatggcg tcgagggtcg ccgggtcggc cgggaagcgc ctcgccgtcc
     9061 aggtgagggt ggcaccggtg gcggcgacga gccggaccag cgtgtcggcc cgggggatgc
     9121 gggtgccccg ctcgtagtgg cacacggctg cggccgaggt gccggcccgc tcggcgagct
     9181 ggcgctgggt cagccccgcc tcggcgcggc agctgcggag gaaggtggcg acgtccacga
     9241 caccatgtta tcggatccga caacgcgatt cttccgagag gtgcttgccc gaacgctcgt
     9301 tcgaagttac gctgctgtcg ttcgccatcg ggcggtccgg ccgggccacg agttcctgtc
     9361 agaccccgtc cgtaggttca ccccacagac gcactcgctg ccccggcagc ctcacctccg
     9421 gccacacgag cgggtcggac cctcgaaagg acgaacagca atggagcgag acaaggcact
     9481 cgagatggct ctcgggaaca tcgagaagca gttcggcaag ggcgccgtca tgaagatggg
     9541 ggagaagggc tcgatgaaca tcgagtcgat ccccaccggc gccctggcgc tcgacctcgc
     9601 cctcggcatc ggcggcctcc cccggggccg ggtcaccgag atctacggcc cggagtcctc
     9661 gggcaagtcc accctcgcca tgcacgtcgt ggccgaggcc cagcgcaacg gcggcatctg
     9721 cgcctacatc gacgccgagc acgccatgga cccggtctac gcccgggcca tcggggtcga
     9781 cgtcgacgag ctgctcatct cccagcccga caccggcgag caggccctcg agatctgcga
     9841 catgctcgtg cgctcggccg ccatcgacgt gatcgtggtc gactcggtcg ccgccctcac
     9901 cccccgcgcc gagatcgagg gggagatggg cgacacccac gtcggcctcc aggcccg
//
LOCUS       DATISU010000437         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_133649, whole genome shotgun sequence.
ACCESSION   DATISU010000437 DATISU010000000
VERSION     DATISU010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133649"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..135
                     /locus_tag="VLR27_17450"
     tRNA            62..135
                     /locus_tag="VLR27_17450"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:96..98,aa:Val,seq:gac)
     gene            500..2224
                     /locus_tag="VLR27_17455"
     CDS             500..2224
                     /locus_tag="VLR27_17455"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HSP05296.1"
                     /translation="MGPGGRLGSTGRDAPRGLTRRALLGAAATSVVASSAVARTLQRA
                     GTESLDGVSLVVAQHELRNDRHRLRDLRSAQRAAGALEPFVLVGITAPGGLPADEEHT
                     AWIRTRGADGWSEWMPIGFSPLERPDDAEAAGGPALASQPLWVGWSDAVEIDVPAGID
                     GSAEVHLLRETPRVLGWHGNDAPAATTHATQPVIQSRASWNAVPYKGTPQIADELKLA
                     IVHHTAGRNGYAYGEVASIIAGIQRYHMESNGWNDIGYNFMVDRFGRVWDARQGGTHN
                     AVIGGHAYGHNTSSTGISVLGNFQEAWPSGAAVEAVTAVAAWKLDHHGCDVASPVWYE
                     SKAAGKSGTYSRIIGHRDVGATSGSTSCPGHRLYPQLGAIRADAHMRQLGLEDIPIDS
                     IYAVAVAWMVRAEITTGIGSPPRFRPLDVVSRGQMALFLWRLMGEPFQVVPHGFLDVP
                     PGSFFDDAVRWLAGAGITGGTEPGRFGPGDPVTRAQMVAFLHRLAGEPGGHPAHGFSD
                     VPFDAYYDGAVRWAKAHGITGGNAEGLFDPQRGVSRGQTALFLHRLASQRAAWAEGAD
                     TPPSALFS"
BASE COUNT          314 a          890 c          833 g          343 t
ORIGIN      
        1 cggaggggcc gatcgacgat gtggcggcgc ccgggcgcgc tgctacagtg cccgctcccg
       61 agggccgtta gctcagttgg ctagagcacc tgcatgacgc gcagggggtc gggggttcga
      121 atccctcacg gcccactcgg aacgcccagg tccacggcct gggcgttgtc gcgtctccgg
      181 tggacggccg acgtgtggac cggtccgtac cgagcggtcc cgatccgtcc atgcgtcccc
      241 gtccgggcag cacgtgtggg ctgtcggcgc ccttccgggt tcgatccgtc cacacgtcgt
      301 cggtgttggt gtagatcggc cgctgccgtt ctcaggacct ttgtccctga tctgcgctcc
      361 gcgccgtctc cgggcaccac ccgctgtagc gttggtgcgg gcaggggcca gcgccggggt
      421 cggtgctgcg cgcgccgggc ggaatccgca catcgagcga ccccgaggac acgagcgaat
      481 gccactccac catgacggga tgggccctgg gggccgtctt ggttcgaccg gccgtgacgc
      541 gccgcgcggc ctcacccggc gagcactgct gggtgctgcc gccacctcgg tggtggcgag
      601 ctccgcggtc gcacggacgc tgcagcgggc aggcaccgag tcgctcgacg gcgtctccct
      661 ggtcgtcgcc cagcacgagc tgcggaacga ccgccaccgc ctccgtgacc tgcgcagcgc
      721 ccagcgcgcc gccggcgcgc tggagccgtt cgtgctcgtc ggcatcaccg ccccgggtgg
      781 gctccccgcc gacgaggagc acaccgcatg gatccgcacc cgcggcgccg acggctggag
      841 cgagtggatg ccgatcgggt tctcgccgct cgagcggcct gacgatgccg aggccgccgg
      901 cggtccggcg ctggccagcc agcccctctg ggtcgggtgg tccgatgccg tggagatcga
      961 cgtgcccgcc ggcatcgacg gctcggccga ggtccacctg ctgcgcgaga cgcctcgcgt
     1021 cctgggctgg cacggcaacg acgccccggc agcgacgacg cacgcgacgc agccggtcat
     1081 ccagtcccga gcgtcgtgga acgccgtgcc gtacaagggc acgccgcaga tcgccgacga
     1141 gctgaagctg gccatcgtgc accacacggc gggtcgcaac ggctacgcct acggcgaggt
     1201 cgcgagcatc atcgccggga tccagcgcta ccacatggag tccaacggct ggaacgacat
     1261 cgggtacaac ttcatggtcg atcgcttcgg ccgggtgtgg gacgcccgcc agggcggcac
     1321 gcacaacgcc gtgatcggcg gccacgccta cggccacaac acctcgagca cgggcatctc
     1381 ggtgctgggc aacttccagg aagcatggcc gtcgggcgca gcggtcgagg cggtcaccgc
     1441 cgtcgccgcg tggaagctcg accaccacgg ctgcgacgtg gcatcgccgg tctggtacga
     1501 gtcgaaggcg gccggcaagt cggggacgta ctcgcgcatc atcggccatc gcgatgtcgg
     1561 cgccacctcc ggctcgacct cgtgtcccgg ccatcgcctc tacccgcagc tgggtgccat
     1621 ccgggccgac gcccacatgc gccagctggg cctcgaggac atcccgatcg actcgatcta
     1681 cgcggtcgcc gtcgcctgga tggtgcgagc cgagatcacc accggcatcg ggtccccgcc
     1741 gcggttccgc cccctcgacg tggtctcccg cgggcagatg gcgctgttcc tgtggcggct
     1801 catgggcgag ccgttccagg tcgtgccgca cggcttcctc gacgtgcccc ccgggtcgtt
     1861 cttcgacgac gccgtccggt ggctggcggg tgccggcatc accggcggta ccgagcccgg
     1921 ccggttcgga cccggcgacc ccgtcacccg cgcccagatg gtggcgttcc tgcaccggct
     1981 cgccggcgaa cccggcgggc accccgccca cggcttcagc gacgtcccct tcgacgcgta
     2041 ctacgacggg gcggtgcgct gggccaaggc gcacggcatc accggtggca acgccgaggg
     2101 cctcttcgat ccccagcggg gcgtgtcccg gggccagacc gcgctgttcc tccaccggct
     2161 cgcatcccag cgcgccgcgt gggccgaggg cgccgacacg ccgccgtcgg cgctgttctc
     2221 ctgaggccgg ggcggacgtc cccttccccg tccggatctc ccgtcgtacc ctgacgggag
     2281 gccgaagggg gaccgacatg gacatccacc agtgcccgcg gtgcgagctg cgcttcgtca
     2341 acacgagtga gctccgccac cacttcgagt tcgaccacgc
//
LOCUS       DATISU010000438         6416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_880401, whole genome shotgun sequence.
ACCESSION   DATISU010000438 DATISU010000000
VERSION     DATISU010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880401"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /gene="lexA"
                     /locus_tag="VLR27_17460"
     CDS             <1..580
                     /gene="lexA"
                     /locus_tag="VLR27_17460"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HSP05297.1"
                     /translation="NHQRERGYPPSVREIGSAVGLTSPSSVHAHLATLQRLGYLQRDP
                     TKPRAIEVRWDPTSGAGVERRPVRHVPLVGDVAAGTDVLAQENITETMPLPADLTGDG
                     DLFMLKVRGDSMIEDGILDGDFVVCRAQNTAQKNDVVVAGIPGDEATVKRFEAVGGEI
                     VLHPANARLEPMRFAPDEVHIYGKVVTVLRTL"
     gene            623..1867
                     /locus_tag="VLR27_17465"
     CDS             623..1867
                     /locus_tag="VLR27_17465"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSP05298.1"
                     /translation="MRPPPRHVLRRAVLATSCVLATVVAPAGVADSGDVANDSGLQWN
                     LDRIGAEAAWATGTGEGTTIAVIDSGVALEHEDLVDKLVDGVACRDTDGDPARCTGSP
                     QDDDGHGTHVAGVAGATTGNGVGIAGTAPDARIMPIKVLFSACDTCQSTGEAEDVSAA
                     VRWAADEGATVINLSLGSTTSSVFGPGFADAVRYAWTKGSIPVVAAGNDYVLTADLGD
                     APAVVVSATAHDDTAPDYSNGVGTARWAVAAPGGDGSDTPESCSQRGEPQGVLSTWWS
                     LADPVGGYACLSGTSMAAPHVSGALAVLRGAGLSPEVAVQRLLDTAVDLGDPGADPIF
                     GAGRIDLAAALADVEAVTTASPTSQPVDPVESPSEDAAAPRPPESDELPVGLVLLAGI
                     LVAGTGAVSAVALTRARRPSRP"
     gene            complement(1923..2981)
                     /gene="dapE"
                     /locus_tag="VLR27_17470"
     CDS             complement(1923..2981)
                     /gene="dapE"
                     /locus_tag="VLR27_17470"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HSP05299.1"
                     /translation="MTDLLEATAELVDIASPSLAEGPFADLVEGWLREVPWLTVDRHG
                     DNVVARTDLGHDQRLVLAGHLDTVPANGNDVARIDGDTLWGLGSADMKGGLAVFLELA
                     RTVEAPSCDLTYVFYAAEEIAAEHSGLLQLFRERPDLVAADAAILGEPTDGWIEAGCQ
                     GTARVRLTLTGTRAHTARPWKGRNAIHRLGPILEAIAGYEAREPTIDGCTYREAMQAV
                     FVEGGVAGNVVPDEAVLTVNLRIAPDRTVDEAVDAFRSLVAPWIEDGDGFEVVDRSEP
                     APPSLTHPLLAPLVEAGTPVRAKLGWTDVARFAEHGIPAINFGPGDSSVAHMRDEHVT
                     RESIERVHAALHALLTAP"
     gene            complement(2986..3825)
                     /locus_tag="VLR27_17475"
     CDS             complement(2986..3825)
                     /locus_tag="VLR27_17475"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HSP05300.1"
                     /translation="MSNSLPEQITELWERRDELSPADTDAFEVVRQAIDQLDRGEVRV
                     ADVTGDGVADDEVTVNEWAKLAILLLFRLTSMETMELGPFEYADKIPLKQGYQDAGVR
                     VVPGASARWGSHLSRGVVMMPSYVNIGAWVGENSMVDTWATVGSCAQIGANVHLSGGV
                     GIGGVLEPANASPVIIGDECMIGSRCIVAEGARVGAGTILGAGCVLTASIPVIDAETG
                     DEISRGRVPAWSVAVSATRPRTFAGGEFGLPCVLVIKRLNEGERHDKSKLNSILRDHG
                     ATV"
     gene            complement(3837..5156)
                     /locus_tag="VLR27_17480"
     CDS             complement(3837..5156)
                     /locus_tag="VLR27_17480"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HSP05301.1"
                     /translation="MGVRTSQPSGRGLRRMVRSLTALALVAGGCAADGPEVPIERAAP
                     PVPRSVTRTPVVETVPAPTTTAPVAPPVVAASAAGSVEVPADPAPAPPPEPVVNRPPP
                     RPDRSAPAPAGPPAPASAESTAVHPADFPDPFVLRANGAYWAFATQVGLTAIPTMRSD
                     DLLRWEPVGDAIRGTPSWAEWGHHWAPAVLPRGSVFVLYYTTRHAATGLQCISRVASV
                     LPQGPYLDDSDGPLVCQTERGGSIDPSPFVDGDGRPYLLWKSEGTLAGEPTRLWVQPL
                     SEDGLHLVGEGPVQLLERRLPWEFPIIEGPSMALVDGRHHLLYSGSRWETSGYAVGHA
                     VCDSVLGPCRRSAPGPVLGSRPGEAGPGGQEVVHLRSGEQVLVHHAWEPGAVGYPGGA
                     RRLHLTSLRIDGDRLAAGPPWRDEARADLVQDVVPPGRQVGGARARH"
     gene            complement(5212..6339)
                     /locus_tag="VLR27_17485"
     CDS             complement(5212..6339)
                     /locus_tag="VLR27_17485"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HSP05302.1"
                     /translation="MSAAAPAPFVPPPYPYDRLDPLRAVAAEHDGGAVDLSIGTPFDP
                     PPPAVTEALGSADARGYPASNGLPAFREAAAGWLRSFLGVDLSTDQVAACIGTKELVA
                     GMPQWLRLRSPERDTVLYPEISYPTYEMGATLAGCRAVPVPVRADWTIDLGGIDDADA
                     ARALCLWVNTPGNPAGGLDDLGAAAEWGRARGVPVLSDECYVAFTWDGPARTILHHGT
                     EGVLAVHSLSKRSNLAGGRAGFYAGDADLVSYLREVRKHAGFMVPSPVQAAAIAALGD
                     EGHVEVQRDRYHRRLLALQAALAGIGIDAPMPGGGFYLWVSAPGGDAWGLTERLARDG
                     GAIVSPGEFYGPAGAGHVRVAAVQPDERIALVVERLAANAP"
BASE COUNT          841 a         2359 c         2412 g          804 t
ORIGIN      
        1 gaaccaccag cgagagcggg gctacccgcc ctcggtgcgc gagatcggca gcgcagtcgg
       61 gctcacctcc ccctcctcgg tccacgccca cctcgccacc ctccagcgcc tcggctacct
      121 gcagcgcgac cccaccaagc cccgcgccat cgaggtccgc tgggacccga cctccggcgc
      181 cggggtcgag cgtcgcccgg tgcgccacgt gcccctggtc ggcgacgtcg ccgccggcac
      241 cgacgtgctc gcccaggaga acatcaccga gaccatgccg ttgcccgccg acctcaccgg
      301 cgacggcgac ctgttcatgc tcaaggtgcg gggcgactcc atgatcgaag acggcatcct
      361 cgacggcgac ttcgtcgtgt gccgggccca gaacaccgcc cagaagaacg acgtcgtggt
      421 ggccggcatc cccggcgacg aggccacggt caagcgcttc gaggcggtcg gcggcgagat
      481 cgtcctgcat ccggccaacg cccgactcga gccgatgcgc ttcgcgccgg acgaggtcca
      541 catctacggc aaggtcgtca cggtcctgcg caccctgtag cgcggcaccc gacgggccgg
      601 ccccatcggc gagaatccgg gggtgcgccc cccgccccgc cacgtcctgc gccgcgccgt
      661 gctggccaca tcctgcgtgc tggcgacggt cgtcgcgccg gccggcgtcg ccgactccgg
      721 cgacgtcgcc aacgacagcg gcctccagtg gaacctcgac cgcatcgggg ccgaggcggc
      781 gtgggccacc ggcaccggtg agggcacgac catcgccgtc atcgactccg gcgtcgcgct
      841 cgagcacgag gacctggtcg acaagctcgt cgacggcgtg gcctgccggg acaccgacgg
      901 tgatccggcc cgctgcaccg ggagccccca ggacgacgac ggccacggca cccacgtggc
      961 cggtgtcgcc ggggcgacca ccggaaacgg ggtggggatc gccggcacgg cgccggatgc
     1021 tcgcatcatg ccgatcaagg tcctgttcag tgcctgcgac acgtgccagt ccaccggtga
     1081 ggccgaggac gtgtccgccg cggtgcggtg ggccgccgac gagggcgcca cggtcatcaa
     1141 cctctcgctc ggctcgacca cctcgtccgt gttcggcccc ggcttcgccg acgccgtccg
     1201 ctacgcctgg acgaagggct cgatcccggt cgtcgcggcc ggcaacgact acgtcctcac
     1261 cgccgatctc ggcgatgccc ccgccgtcgt ggtgtccgcc accgcccacg acgacaccgc
     1321 gccggactac tccaacggcg tgggcacggc gcggtgggcg gtggccgcgc cgggcggcga
     1381 cggcagcgac accccggagt cgtgctcgca gcggggcgag ccccaggggg tcctgtccac
     1441 gtggtggtcg ctggccgacc cggtcggggg ctacgcctgc ctgtccggga cgtcgatggc
     1501 ggccccccac gtcagcggcg ccctcgccgt gctccgcggt gctggcctgt cgcccgaggt
     1561 ggcggtgcaa cgcctcctcg acacggcggt ggacctcggc gaccccggtg ccgatccgat
     1621 cttcggcgcc ggccgcatcg acctcgccgc cgccctcgcc gatgtcgagg ccgtgaccac
     1681 cgcatcgccg acctcgcagc ccgtggaccc cgtcgagtcg ccgtccgagg acgccgcggc
     1741 accgcgacct cccgagagcg atgagctgcc cgtcggcctc gtgctgctcg ccggcatcct
     1801 cgtcgccggg accggggccg tctcggccgt ggcgctcacc cgagcccgtc gcccgtcccg
     1861 accgtgatgg ttctggccta cgtccgttcc acctggtggt cgtcgatgta ggccagaacg
     1921 gatcaggggg cggtgagcag ggcgtggagg gcggcgtgca cccgctcgat ggactcgcgg
     1981 gtgacgtgct cgtcccgcat gtgggccacc gaggagtcgc cgggcccgaa gttgatcgcc
     2041 gggatgccgt gctcggcgaa gcgggccacg tcggtccacc ccagcttggc ccgcaccggc
     2101 gtgccggcct cgaccagtgg tgccagcagc gggtgggtca acgacggcgg cgccggctcg
     2161 gagcggtcga cgacctcgaa gccgtccccg tcctcgatcc acggggccac cagggagcgg
     2221 aaggcgtcga cggcctcgtc gacggtgcgg tccggcgcga tgcgcaggtt gacggtgagc
     2281 acggcctcgt ccggcacgac gttgccggcc acgcccccct cgacgaacac ggcctgcatg
     2341 gcctcccggt aggtgcaacc gtcgatggtg ggctcgcgcg cctcgtagcc ggcgatggcc
     2401 tcgaggatcg ggccgaggcg gtggatggcg ttgcgcccct tccacggccg ggccgtgtgg
     2461 gcccgggtgc cggtgagggt cagccgcacc cgtgcggtgc cctggcagcc ggcctcgatc
     2521 cagccgtcgg tcggctcgcc gaggatggcg gcgtcggcgg cgaccaggtc gggacgctcc
     2581 cggaacagct ggagcaggcc gctgtgctcg gcggcgatct cctcggcggc gtagaagacg
     2641 taggtgaggt cgcagctcgg cgcctcgacg gtgcgagcca gctcgaggaa gacggcgagg
     2701 ccgcccttca tgtccgccga cccgagcccc cacagcgtgt cgccgtcgat gcgggccacg
     2761 tcgttgccgt tggccggcac ggtgtcgagg tgcccggcga gcaccaggcg ctggtcgtgc
     2821 ccgaggtcgg tgcgcgccac gacgttgtcg ccgtggcggt cgacggtgag ccacgggacc
     2881 tcgcgcaacc acccctcgac gaggtcggcg aaggggccct cggccaggct gggcgaggca
     2941 atgtcgacca gctcggcggt ggcctcgagg agatccgtca ccggctcaga cagtggcgcc
     3001 gtggtcgcgc aggatgctgt tgagcttgga cttgtcgtgg cgctcgcctt cgttgaggcg
     3061 cttgatgacc agcacgcacg gcaggccgaa ctcgccgccg gcgaaggtcc gggggcgggt
     3121 ggccgagacc gccacgctcc aggccgggac ccggccccgg ctgatctcgt cgccggtctc
     3181 ggcgtcgatg accgggatcg aggcggtgag cacgcagccg gcaccgagga tggtgccggc
     3241 cccgacccgg gcgccctcgg ccacgatgca gcggctgccg atcatgcact cgtcgccgat
     3301 gatcacgggc gaggcgttgg ccggctcgag gacgccgccg atgccgacgc cgccggagag
     3361 gtgcacgttg gcgccgatct gggcgcagga gccgacggtg gcccaggtgt cgaccatgga
     3421 gttctcgccc acccaggcgc cgatgttgac gtaggacggc atcatcacga cgccccggga
     3481 caggtggctg ccccagcggg ccgaggcgcc gggcacgacc cggaccccgg cgtcctggta
     3541 gccctgcttc agcgggatct tgtcggcgta ctcgaacggg ccgagctcca tcgtctccat
     3601 cgaggtgagc cggaacagca gcaggatcgc cagcttggcc cactcgttga ccgtgacctc
     3661 gtcgtcggcc acgccgtcac cggtgacgtc ggcgacccgg acctcgccgc ggtcgagctg
     3721 gtcgatcgcc tggcggacca cctcgaaggc gtcggtgtcg gccggcgaga gctcgtcccg
     3781 gcgctcccag agctcggtga tctgttcggg cagcgagttc gacatggagg gcgaatctag
     3841 tggcgagccc gcgcaccgcc gacctggcga cccggaggga cgacgtcctg cacgagatcg
     3901 gcgcgcgcct catcgcgcca cggcggccca gccgccagtc ggtcgccgtc gatgcgcagc
     3961 gacgtgaggt gcagccgtct cgccccgccc gggtagccga ctgcgccggg ctcccaggcg
     4021 tggtggacca gcacctgctc acctgaccgc aggtggacga cctcctggcc accgggcccg
     4081 gcctcccccg gccgtgatcc gaggaccgga ccgggcgcgc tgcggcggca cggcccgagg
     4141 acggagtcgc acacggcgtg gccgaccgcg taccccgacg tctcccagcg gctcccggag
     4201 tagaggaggt ggtggcgacc gtcgacgagc gccatcgacg ggccctcgat gatcgggaac
     4261 tcccagggca ggcgtcgctc gagcagctgg accggaccct cgccgacgag gtgcaggccg
     4321 tcctcgctga gcggctgcac ccagaggcgg gtgggctcgc cggccagcgt cccctcgctc
     4381 ttccacagca ggtacggccg gccgtcgccg tcgacgaagg ggctgggatc gatcgagccg
     4441 ccccgctcgg tctggcagac gagcggcccg tcgctgtcgt cgaggtacgg gccctggggc
     4501 aggacgctgg cgacccgcga gatgcactgc aggcccgtcg ccgcgtggcg ggtcgtgtag
     4561 tagaggacga agaccgaccc tcgcgggagc acggccggcg cccagtggtg accccactcc
     4621 gcccacgacg gcgtcccccg gatggcatcg ccgacgggtt cccagcggag caggtcgtcg
     4681 gagcgcatgg tcgggatcgc cgtgaggccg acctgggtgg cgaaggccca gtaggcgccg
     4741 ttggcccgga gcacgaaggg gtccgggaag tcggccgggt gcaccgccgt gctctccgcc
     4801 gaggccggtg ccggtgggcc ggcgggcgcc ggcgcggacc ggtccggccg gggcgggggc
     4861 cggttcacga ccggttcggg tgggggcgcc ggggctgggt cggcgggcac ctcgaccgaa
     4921 ccggccgccg aggcggccac gaccggcggt gccaccgggg cggtggtcgt cggcgccggc
     4981 accgtctcga cgaccggggt gcgggtcacc gaccggggca cgggtggggc tgctcgttcg
     5041 atcggcacct cgggaccgtc cgccgcgcac ccgccggcca ccagggcgag cgccgtcagc
     5101 gaccgcacca tccggcgcag gccccggccc gacggctgcg atgtcctgac ccccacgaga
     5161 cgtccttcgc cccggggcac ggcccgtcct gctacgtgcg cactgccagc ttcagggagc
     5221 gttggcggcg aggcgctcga ccacgagggc gatgcgctcg tcgggctgca cggcggcgac
     5281 ccgcacgtgg ccggcgccgg cggggccgta gaactcgccg gggctgacga tggcgccgcc
     5341 gtcgcgagcg aggcgctcgg tgaggcccca cgcatcgcca cccggggccg acacccagag
     5401 gtagaagcca ccgccgggca tgggggcgtc gatgccgatg ccggcgagcg cggcctgcag
     5461 cgccagcaga cgccggtggt agcggtcgcg ctggacctcg acgtggccct cgtccccgag
     5521 tgcggcgatg gcggcggcct gcaccggcga gggcaccatg aacccggcgt gcttgcgcac
     5581 ctcgcgcagg tacgagacga ggtcggcgtc gccggcgtag aagccggccc ggccgccggc
     5641 caggttggag cgcttcgaca gcgagtggac ggcgaggacg ccctcggtgc cgtggtggag
     5701 gatcgtgcgc gccgggccgt cccaggtgaa cgccacgtag cactcgtcgg acagcaccgg
     5761 gacgccacgg gcccggcccc actcggccgc ggcaccgagg tcgtcgaggc ccccggccgg
     5821 gttccctggg gtgttcaccc acaggcacag cgcccgggcg gcatcggcgt cgtcgatgcc
     5881 gccgaggtcg atcgtccagt ccgcccggac ggggacgggg acggcacggc agccggcgag
     5941 ggtggcaccc atctcgtacg tcgggtagct gatctcgggg tacagcaccg tgtcgcgctc
     6001 ggggctccgc agccggagcc actgcggcat gcccgcgacc agctccttgg tgccgatgca
     6061 ggcggcgacc tggtcggtgg agaggtcgac gccgaggaag ctccgcagcc agccggccgc
     6121 ggcttcgcgg aaggcgggca ggccgttgga cgccgggtag ccccgggcgt cggccgagcc
     6181 gagcgcctcg gtgaccgccg gcggaggcgg gtcgaagggg gtgccgatgc tgagatcgac
     6241 cgcaccaccg tcgtgctcgg ccgccaccgc acggagcgga tcgagccggt cgtaggggta
     6301 gggcgggggg acgaacgggg ccggcgcggc agcgctcact ccgactcctc gacctcgaac
     6361 tcgcggaact gggcccggga cgccgcgtcc atgcgggcct ggacgacggt gccggt
//
LOCUS       DATISU010000439         6959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_600878, whole genome shotgun sequence.
ACCESSION   DATISU010000439 DATISU010000000
VERSION     DATISU010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600878"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /gene="ndhC"
                     /locus_tag="VLR27_17490"
     CDS             complement(<1..306)
                     /gene="ndhC"
                     /locus_tag="VLR27_17490"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSP05303.1"
                     /translation="MSEFLRNYLTVAIFGGVAVALLAGVLGLGSLIRPTRRQDGKYEN
                     YESGVDPVGDTWSQSQIRYYIFALMFVIFDVEAVFIFPWATRVEEYGVFGLVEMLVFI
                     "
     gene            complement(419..1255)
                     /locus_tag="VLR27_17495"
     CDS             complement(419..1255)
                     /locus_tag="VLR27_17495"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HSP05304.1"
                     /translation="MAKQRAPHRAGARAVLLLTIGVALGFGAYHAVRSLDVTDAESTV
                     ADGPTPTTEVSTTAAPTTGAPTTTPPPTTAAPTTAAPTTTAPPTTVPGPPSYRAGDGG
                     PEVAALQERLVALGFWVPAVDGDYGSVTQQAVMAFQKHAGLSRDGIAGPATLAALDVA
                     PPVAAREGGDHVELDLERQLLIVVRDGRTTAFNTSTGTSGWRTPPGRFIVQREIDGVR
                     EAPLGDLYRPKYFNAGIAVHGSPSIPGQPASHGCARVHDRVMDLLWSGGLMPVGTPVW
                     VY"
     gene            complement(1333..2088)
                     /locus_tag="VLR27_17500"
     CDS             complement(1333..2088)
                     /locus_tag="VLR27_17500"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSP05305.1"
                     /translation="MTLAESVLIAVAGFLAGGVNAVAGGGSLISFPALVAAGLPTLDA
                     NVTNTTAVWPGYLGSAAAYRSELRTQGPRLRALAVTAVLGAGAGSAILLAAPDAVFDA
                     VVPFLVLAGSVLLALQPRIAKAVLARTEAGGQGQTAQLHVAIFLAAVYGAYFGGGLGV
                     ILLAVLGIFVADHLQRLNGLKAAVSLLVNTVALVAFALFGPVDWSAVAIVAPASLLGG
                     YVGGRLARRLSPDALRTTIVVFGVVVAAWLALR"
     gene            complement(2085..2426)
                     /locus_tag="VLR27_17505"
     CDS             complement(2085..2426)
                     /locus_tag="VLR27_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05306.1"
                     /translation="MASIQSAGDVVEVEDVLVTISPRTGLLARRRSTLEAPVTWPAPR
                     TTVTLHRTDLTGQWQARFTYEGQIVTLVFPPKRLDALVAFAEAHLGGMTNTVMDAEQD
                     RLARRAEDGLR"
     gene            complement(2440..3993)
                     /locus_tag="VLR27_17510"
     CDS             complement(2440..3993)
                     /locus_tag="VLR27_17510"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HSP05307.1"
                     /translation="MHALLANLTAPLQAADEAFDPIATPEIEWFTIVPLLVLVGAALG
                     LMTLTAITRRPLFHGFYPLATSVAAIGAAVCTWPIWDRIQDPERGPFTALAGAVGVDG
                     FSVFMTVVICSSVVIASLLADGYLRREGLDGPELYVLLLLSASGGVVMAAANDLIVLF
                     LGLEILSLAVYTLAAMHLRRVTSQEAGLKYFVLGSFASAFLLYGIALTYGATGSTNLV
                     EILSYLSQVALFDNGLLLAGMAMLLVGLLFKVAAAPFHAWTPDVYQGAPSPVVAYMAS
                     GVKAAGFAGLIRVLYLGFQTYRVDWQPVIYAVAILTLLVGALMAVVQTDVKRMLAYSS
                     INHAGFVLVAVQAATERGVQASMFYLASYTFLVGGSFAIITIVGRTGDDRHTLDDYRG
                     LAKARPALALVFALLLFSQAGVPFTSGFFAKFYAIAAAVDAGSYPLAAVAMLTAVIAA
                     FLYLRITVSMFLGDVDDADIPERRTLPVPVGAAIALVICVVVTLGVGIFPHVLTGPAG
                     DATAVLVGF"
     gene            complement(3996..5771)
                     /locus_tag="VLR27_17515"
     CDS             complement(3996..5771)
                     /locus_tag="VLR27_17515"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSP05308.1"
                     /translation="MLAANLAFPLLTASILVPLVGALLIAIVPATRREIVRPLAVLFS
                     ATTGAFTLWMLGAFDRGDAGFQFETSHAWIEEFGIGWNLGVDGISLFLVVLSGVLFPL
                     TFVAVTPKHDPKAYYAWLLMLQAGCIGAFSALDLFLFFIFFEIVLVPMYFLIGGWGYG
                     QRIYAALKFFLFTMFGSALMLVGIVSLAFLDRDAALERSAADTEELAGLQESIDVRES
                     SGVDVPQEEYDRAEELTASVEGADQLTFDLVEIAEGRAEALAADQEAIEAGEMEARDS
                     WNPLDWEAAKWIFLAFALAFAVKVPLFPLHTWLPDAHTQAPTAGSVILAGVMLKLGTY
                     GFVRFGLYLFPDASLFFAPVIATLGVIGILYGAVVATMQKDIKRLVAYSSVAHLGFIA
                     LGTFAMTTQGIGGGVLQMVNHGISTGALFLLLGMLYERRHTYDIAAMSGLQKATPVLA
                     GVFTLVMLSSIGLPGLNGFVGEFLVLAGAYLTLKWWAVVAAAGVILAALYLLWAYQRV
                     FHGEPTEEDLATPDMRIGELAYMAPLLALIVFLGVYPKPVLERIEPSVAALVSHIEGA
                     TDFVEPDAATSGFSGTTADDHGEGE"
     gene            complement(5771..>6959)
                     /locus_tag="VLR27_17520"
     CDS             complement(5771..>6959)
                     /locus_tag="VLR27_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSP05309.1"
                     /translation="TWIQPTIAWVGAGTALFAATIAVSQKDIKKVLAYSTVSQLGYMF
                     LAVGVGAYVPAVFHMITHAFFKALLFLGSGSVIHGMHEEQDMTRMGGLRKYMPITAAT
                     FIVGWLAIAGVPPFSGFFSKDEILLYAWAENPALWAVGLVTALLTAFYMSRQVFLVFF
                     GDERFTVDAHHGDHDEVAAVDEGDADTALDTEGSEAEALERPATADAPQHDGVHVLHA
                     EPHESPWTMTLPLIALAGLAMVGGAIQLPFTKDLHFLEDWLHPVLGDNEAHIDVATGT
                     KVALAVVAALIALTGIAIAWAIYVRKLRRPVEPAILEEGWRYDRAITSFMGGPGRAGF
                     EAASTFDRTVVDGAVNGVGSLVRRSGGGLRGLQSGFVRNYALGIAGGAVLLLAYFLAQ
                     VTL"
BASE COUNT         1183 a         2199 c         2742 g          835 t
ORIGIN      
        1 gatgaagacg agcatctcga cgaggccgaa gacgccgtac tcctcgacgc gggtggccca
       61 cgggaagatg aagaccgcct ccacgtcgaa gatgacgaac atgagggcga agatgtagta
      121 ccggatctgg ctctgcgacc aggtgtcgcc gaccgggtcg acgccggatt cgtagttctc
      181 gtacttgccg tcctggcgac gggtcggacg gatgagcgaa ccgaggccga ggacgccggc
      241 caacagcgcg accgcaacgc caccgaagat ggcaaccgtc aggtaattac ggaggaactc
      301 cgacacggag gggcacccta ctgaccggcg gagaagtggg ccaagatcgc ccaaccgggc
      361 cccagacgac ccatccgggc ccgaattccg ggcacctgcg gccggtccga ggccccgctc
      421 agtacaccca gaccggcgtc ccgacgggca tcaggccgcc cgaccagagc aggtccatga
      481 cccggtcgtg gacccgcgcg cagccgtggg aggcaggctg gcccgggatg ctcggggagc
      541 cgtgcacggc gatgccggcg ttgaagtact tgggccggta gaggtcgccg agcggcgcct
      601 cccggacgcc gtcgatctcc cgctggacaa tgaagcggcc cggcggggtc cgccagcccg
      661 aggtgccggt ggaggtgttg aacgccgtcg tgcgcccgtc gcgcacgacg atgagcagct
      721 ggcgctcgag gtcgagctcg acgtggtcgc ctccctcgcg cgccgccacc gggggtgcga
      781 cgtcgagtgc ggccagcgtg gcgggcccgg cgatgccgtc ccgcgagagt ccggcgtgct
      841 tctggaaggc catgaccgcc tgctgggtca ccgatccgta gtcgccgtcg accgccggca
      901 cccagaaccc gagggcgacg aggcgctcct gcagggcggc cacctccggt cccccgtcac
      961 cggcgcggta ggacggtggg ccggggacgg tggtgggcgg ggcggtggtg gtgggagcgg
     1021 ccgtggtcgg cgctgcggtg gtcgggggcg gagtggtggt gggtgccccg gtggtcgggg
     1081 cagcggtcgt cgacacctcg gtggtggggg tgggaccgtc ggccaccgtg gactcggcgt
     1141 cggtgacgtc gagggatcgc acggcgtggt aggcgccgaa gccgagcgcc acgccgatcg
     1201 tgaggagcag cacggcgcgt gcgccggcgc ggtggggggc tcgttgcttc gccatcgatg
     1261 atcagacgct ccagccctgt ccgccgttca cgggaaatgt atgacatcgg tcacggcagg
     1321 accagcgcgc gctcatcgca gcgccagcca ggctgcgacc acgaccccga acacgacgat
     1381 ggtggtgcgc agcgcatccg gcgagaggcg gcgggccagg cggccgccga cgtagccgcc
     1441 gagcaggctc gccggcgcca cgatcgccac cgcgctccag tcgaccgggc cgaacagggc
     1501 gaaggcgacg agcgcgacgg tgttgaccaa cagggagacg gcggccttca gaccgttgag
     1561 ccgctgcagg tggtcggcga cgaagatgcc gaggacggcg aggaggatca cgcccaggcc
     1621 accgccgaag taggcgccgt acacggcggc caggaagatg gcgacgtgga gctgggcggt
     1681 ctggccctgg cccccggcct cggtgcgggc caggaccgcc ttggcgatgc ggggctgcag
     1741 ggcgagcagc accgacccgg ccagcacgag gaagggcacc acggcgtcga agacggcatc
     1801 gggggcggcg agcaggatcg ccgagccggc cccggcgccg agcacggccg tgacggcgag
     1861 cgcccggagg cggggaccct gcgtgcgcag ctcgctgcgg taggcggcgg cgctgcccag
     1921 gtagccgggc cacaccgcgg tggtgttggt gacgttggcg tcgagggtcg gcagcccggc
     1981 tgcgacgagg gccgggaagg agatgagcga cccgccaccg gcgacggcgt tgacgccgcc
     2041 ggcgaggaac ccggcgaccg cgatcagcac cgactcggcg agggtcatcg caacccgtcc
     2101 tcggcgcggc gggcgaggcg gtcctgctcg gcgtccatga cggtgttggt catgccgccg
     2161 aggtgggcct cggcgaaggc gacgagggca tcgaggcgct tgggcgggaa caccagggtg
     2221 acgatctggc cctcgtaggt gaaccgggcc tgccactgcc cggtgaggtc ggtgcggtgc
     2281 agcgtcacgg tggtccgggg cgccggccac gtcaccggcg cctcgagcgt ggaccgccgc
     2341 cgggccagga gaccggtgcg gggcgagatg gtcaccagca cgtcctcgac ctcgacgacg
     2401 tcgccggccg attggatgga tgccacggcc ggcggcgggt cagaagccga cgaggaccgc
     2461 ggtggcgtcg ccggcggggc cggtgagcac gtgcgggaag atgcccacgc cgagggtcac
     2521 gaccacacag atgacgaggg cgatggcggc gccgaccggc accggcagcg tgcgccgctc
     2581 ggggatgtcg gcgtcgtcga cgtcgccgag gaacatcgac accgtgatgc gcaggtacag
     2641 gaaggcggcg atgacggcgg tgagcatcgc gaccgcagcc aagggatacg agccggcgtc
     2701 cacggcggcg gcgatggcgt agaacttcgc gaagaagccg ctggtgaagg gcacgcccgc
     2761 ctggctgaac agcagcaggg cgaagaccag ggcgagggcg gggcgggcct tggccaggcc
     2821 ccggtagtcg tcgagggtgt ggcggtcgtc gccggtgcgc ccgacgatgg tgatgatggc
     2881 gaagctgccg ccgaccagga acgtgtagct ggccaggtag aacatcgacg cctgcacgcc
     2941 ccgctcggtg gcggcctgca cggccacgag cacgaagccg gcgtggttga tcgaggagta
     3001 cgccagcatc cgcttcacgt cggtctgcac gacggccatc agggcaccga cgagcagggt
     3061 gaggatggcc acggcgtaga tcaccggctg ccagtcgacc cggtaggtct ggaagccgag
     3121 gtagagcacc cggatgaggc cggcgaaccc ggccgccttc acgccggagg ccatgtaggc
     3181 caccaccggc gacggggcgc cctggtacac gtcgggggtc caggcgtgga acggggcggc
     3241 ggcgaccttg aagaggaggc cgaccaggag catcgccatg ccggcgagca ggaggccgtt
     3301 gtcgaacagg gcgacctggc tgaggtagct gaggatctcg accaggttgg tgctgccggt
     3361 ggcgccgtag gtgagggcga tgccgtacag caagaacgcg gaggcgaacg agccgagcac
     3421 gaagtacttg aggccggcct cctgggaggt gacccggcgc aggtgcatgg cggcgagcgt
     3481 gtagacggcg agcgagagga tctcgaggcc gaggaacagc acgatgaggt cgttggccgc
     3541 cgccatcacc acgccgccgg aggcggagag caggagcagc acgtacagct cggggccgtc
     3601 gaggccctcg cgtcgcaggt agccgtcggc cagcagcgag gcgatgacca ccgaggagca
     3661 gatgaccacg gtcatgaaga ccgagaagcc gtcgacgccg accgcgccgg cgagggcggt
     3721 gaaggggccc cgttcggggt cctggatacg gtcccagatc ggccacgtgc acacggccgc
     3781 accgatggcg gcgaccgagg tggcgagggg gtagaagccg tggaacaggg gccggcgggt
     3841 gatcgccgtg agggtcatca ggccgagcgc cgcaccgacc aggaccagca gcgggacgat
     3901 cgtgaaccac tcgatctcgg gcgtcgcgat ggggtcgaag gcctcgtcgg cggcctggag
     3961 cggcgcggtc aggttcgcga gcagcgcatg catggtcact cgccctcccc gtggtcgtca
     4021 gcggtcgtcc ccgagaaccc ggaggtggcg gcgtcgggct cgacgaagtc ggtcgcgccc
     4081 tcgatgtggc tgacgagcgc ggccacggac ggctcgatgc gctcgagcac cggcttcggg
     4141 tagacgccca ggaacacgat gagggcgagc agcggggcca tgtaggcgag ctcgccgatg
     4201 cgcatgtccg gcgtggccag gtcctcctcg gtgggctcgc cgtggaacac gcgctggtac
     4261 gcccacagca ggtagagcgc ggcgaggatg acgccggcgg cggccaccac ggcccaccac
     4321 ttgagggtga ggtaggcgcc ggccagcacc aggaactcgc cgacgaagcc gttgaggccg
     4381 ggcaagccga tcgaggacag catcacgagc gtgaacacgc cggcgagcac gggggtcgcc
     4441 ttctgcaggc cggacatggc ggcgatgtcg taggtgtggc ggcgctcgta gagcatgccg
     4501 agcaggagga acagcgcgcc ggtggagatg ccgtggttca ccatctgcag cacgccgccg
     4561 ccgatgccct gggtggtcat ggcgaaggtg ccgagggcga tgaagccgag gtgggccacc
     4621 gaggagtagg cgacgaggcg cttgatgtcc ttctgcatgg tggccacgac cgcgccgtac
     4681 aggatgccga tgacgccgag cgtggcgatc accggcgcga agaacagcga cgcgtcgggg
     4741 aagaggtaca gcccgaagcg cacgaagccg tacgtgccca gcttcagcat cacaccggcc
     4801 aggatcaccg acccggcggt gggcgcctgg gtgtgggcgt cgggcagcca ggtgtgcagc
     4861 gggaacagcg gcaccttcac cgcgaaggcc agcgcgaagg cgaggaagat ccacttcgcc
     4921 gcctcccagt ccagcgggtt ccaggagtcc cgggcctcca tctcgccggc ctcgatggcc
     4981 tcctggtcgg cggccagggc ctcggcgcgg ccctcggcga tctcgaccag gtcgaaggtg
     5041 agctggtcgg cgccctcgac cgacgcggtg agctcctcgg cgcggtcgta ctcctcctgg
     5101 gggacgtcga cgccggagga ctcgcggacg tcgatgctct cctgcaggcc ggccagctcc
     5161 tcggtgtcgg ctgcgctgcg ctcgagggcg gcgtcgcggt cgaggaaggc gaggctgacg
     5221 atgccgacca gcatcagagc cgagccgaac atggtgaaga ggaagaactt cagcgccgcg
     5281 tagatgcgct ggccgtagcc ccacccgccg atgaggaagt acatcggcac gagcacgatc
     5341 tcgaagaaga tgaagaacag gaacaggtcc agcgcgctga aggcaccgat gcagccggcc
     5401 tgcagcatga gcagccaggc gtagtacgcc ttcggatcgt gcttgggggt caccgccacg
     5461 aacgtgagcg ggaacagcac gccgctgagc acgacgagga acagcgagat gccgtcgacg
     5521 ccgaggttcc agccgatgcc gaactcctcg atccaggcgt gggaggtctc gaactggaag
     5581 ccggcgtcgc cgcggtcgaa cgcaccgagc atccacaggg tgaacgcgcc ggtcgtggcc
     5641 gagaacagca cggcgagagg ccggacgatc tcccggcggg tggccggcac gatggcgatc
     5701 agcagcgcac cgacgagcgg gacgaggatc gacgccgtga gcagcgggaa ggcgaggttg
     5761 gcggcgagca tcagagcgtg acctgcgcga ggaagtaggc gagcaggagg accgcgccgc
     5821 cggcgatgcc gagggcgtag ttgcggacga agccggactg caggccgcgc aggccgccgc
     5881 cggagcggcg caccagcgag cccacgccgt tgacggcgcc gtcgacgacc gtgcggtcga
     5941 aggtggacgc cgcctcgaac ccggcgcggc ccgggccgcc catgaacgag gtgatggccc
     6001 ggtcgtagcg ccagccctcc tcgaggatgg cgggctcgac gggccggcgc agcttgcgca
     6061 cgtagatggc ccaggcgatg gcgatgccgg tgagggcgat cagcgcggcg acgacggcca
     6121 gcgcgacctt ggtgccggtg gcgacgtcga tgtgggcctc gttgtcaccg agcacggggt
     6181 ggagccagtc ctcgaggaag tggaggtcct tggtgaacgg cagctggatg gcgccaccga
     6241 ccatggcgag gccggccagg gcgatgaggg ggagcgtcat cgtccagggg gactcgtggg
     6301 gctcggcgtg caggacgtgg acgccgtcgt gctggggcgc atccgcggtg gcggggcgct
     6361 cgagcgcctc ggcctcggac ccctcggtgt cgagcgcggt gtcggcgtcg ccctcgtcga
     6421 cggcggcgac ctcgtcgtgg tcgccgtggt gggcgtcgac ggtgaagcgc tcgtcgccga
     6481 agaagacgag gaagacctgg cggctcatgt agaacgcggt gagcagggcg gtgaccaggc
     6541 cgacggccca cagcgccggg ttctcggccc aggcgtagag caggatctcg tccttggaga
     6601 agaagcccga gaacgggggc acaccggcga tggcgagcca gccgacgatg aaggtggccg
     6661 cggtgatcgg catgtacttg cgcagaccgc ccatgcgggt catgtcctgc tcctcgtgca
     6721 tgccgtggat gaccgagccg gagccgagga acagcagcgc cttgaagaag gcgtgcgtga
     6781 tcatgtggaa cacggccggc acgtaggcgc cgacgccgac ggccaggaac atgtagccga
     6841 gctgggagac cgtggagtac gccagcacct tcttgatgtc cttctgggac acggcgatgg
     6901 tggcggcgaa cagcgcggtg ccggcaccga cccaggcgat ggtgggctgg atccaggtg
//
LOCUS       DATISU010000440         1524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_135133, whole genome shotgun sequence.
ACCESSION   DATISU010000440 DATISU010000000
VERSION     DATISU010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135133"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VLR27_17525"
     CDS             <1..203
                     /locus_tag="VLR27_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05310.1"
                     /translation="WSPGSGYNVVTGFDRVGMAWHVAYLAGIAVTLAGVALARHTRAP
                     LVGRLVTVGLVVAVGAGFAQVA"
     gene            200..778
                     /locus_tag="VLR27_17530"
     CDS             200..778
                     /locus_tag="VLR27_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05311.1"
                     /translation="MTSVAGQLLRHPVQRRLCAPAAATAAGASVVLALLAELRAQAVP
                     DAVQLAALVLALATVTAATDPVGVLADASPTPGGRRLAVRAGPVLTTVAAGWLLAIAA
                     LPSALRPPVSVGSATLAALVALVVAGGVLEARRRPRATEPLTAAAAVAMGWTALWLLR
                     PSWSPLLGTDPWASVLIAAVGAVAAVRIASPG"
     gene            complement(858..1289)
                     /locus_tag="VLR27_17535"
     CDS             complement(858..1289)
                     /locus_tag="VLR27_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05312.1"
                     /translation="MTRAVLDDHLLRDLLADDVSEGLAEVLAAHEPATTNLYLYRLSK
                     SVVAAPGGALTGRWSPERRRALGSKLLSLPNEVAIVPMSALSYRMAEIAAAHRVSTLG
                     AECVAAAEHLGAPLYVWEGDDGPGIRAAMDHLDLHYRTVGR"
     gene            complement(1286..>1524)
                     /locus_tag="VLR27_17540"
     CDS             complement(1286..>1524)
                     /locus_tag="VLR27_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05313.1"
                     /translation="MEIQTYLVSSSGQMSLPAGARHRWSLDDGGPVDVIDLGFGVLTV
                     PSGEGRKLLGDLLPRDQHAEFVRTLGDDPDLATT"
BASE COUNT          173 a          573 c          563 g          215 t
ORIGIN      
        1 agtggtcgcc gggctctggc tacaacgtcg tcaccgggtt cgaccgggtg ggcatggcct
       61 ggcacgtcgc ctacctggcg ggcatcgccg tgaccctcgc cggcgtcgcg ctggcccgcc
      121 acacccgggc accgctcgtg ggccggctcg tcaccgtcgg cctggtggtc gccgtcggcg
      181 ccggcttcgc ccaggtcgca tgacctccgt cgccggccag ctgctgcgcc acccggtgca
      241 gcgccggttg tgcgcaccgg cggccgccac cgccgccggc gcgagcgtgg tgctggccct
      301 gctcgccgag ctccgggccc aggccgtgcc cgatgccgtc cagctcgccg ccctcgtgct
      361 cgcgctggcc acggtcaccg ccgccaccga cccggtgggt gtgctggccg acgccagccc
      421 cacgccgggc ggccggcgtc tggcggtgcg ggccgggccg gtgctgacca ccgtggcggc
      481 cggttggctg ctcgccatcg cggccctccc atcggcgctg cgcccacccg tctcggtcgg
      541 ctccgcaacc ctggcggcac tcgtcgccct cgtcgtcgcc ggcggtgtcc tcgaggcccg
      601 ccggcgcccc cgagcgaccg agcccctgac ggcagctgcg gcggtcgcca tggggtggac
      661 ggcactgtgg ctgctccgcc cctcgtggtc gccgctgctc gggacggacc catgggcgtc
      721 ggtgctcatc gccgcagtcg gcgccgtcgc cgccgtgcgc atcgccagcc cggggtgagg
      781 cggcgggcgc acagctggtg ccctggcggc ttcccggcgc gaccggccac aggacgccag
      841 ggcccgcaga cggcggctca gcggccgacc gtccggtagt ggaggtcgag gtggtccatt
      901 gcagcccgga tgcccggtcc gtcgtcgcct tcccacacgt agagcggggc tccgaggtgc
      961 tcggctgcgg cgacgcactc ggccccgagc gtcgacacgc ggtgcgctgc ggcgatctcg
     1021 gccatgcggt acgacagtgc gctcatgggc acgatcgcga cttcgttcgg gaggctcagg
     1081 agcttcgaac ccagagctcg acggcgttcg gggctccagc gaccggtcaa cgccccgccc
     1141 ggagctgcca ccacgctctt ggacagccgg tagaggtaga ggttcgtcgt ggccggttcg
     1201 tgcgcggcga gcacctcagc aagaccctcg gacacgtcat cggccaggag atcgcgcagc
     1261 aggtggtcgt cgaggacggc gcgcgtcatg tcgtggcgag gtcggggtcg tcgccgaggg
     1321 tgcgcacgaa ctcggcgtgc tgatcccgag gcaggagatc cccgagcagc ttgcgtccct
     1381 caccactggg gaccgtcagc acgccgaacc cgagatcgat cacgtcgacg gggccgccgt
     1441 cgtcgaggct ccagcggtgc cgggcgccgg cggggaggga catctgaccc gacgaggaca
     1501 cgaggtacgt ctggatctcc atga
//
LOCUS       DATISU010000441         1894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_229369, whole genome shotgun sequence.
ACCESSION   DATISU010000441 DATISU010000000
VERSION     DATISU010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229369"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /gene="dcd"
                     /locus_tag="VLR27_17545"
     CDS             complement(<1..486)
                     /gene="dcd"
                     /locus_tag="VLR27_17545"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HSP05314.1"
                     /translation="MILSDHTIREELANGRIVIDPLDESFIQPSSVDLTLDPRFLVFR
                     NHTRGMIDVKEDASDLTEMITVEDDQPFVLHPGEFVLGATLERIGVPEDLVARLDGKS
                     SLGRLGLLIHSTAGFIDPGFYGQITLELSNVHNLPITLYPKMKIGQVSFLRMTTAADV
                     PY"
     gene            complement(518..820)
                     /locus_tag="VLR27_17550"
     CDS             complement(518..820)
                     /locus_tag="VLR27_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05315.1"
                     /translation="MEQSREGTIIARVGRGDIEIDLAADSAMREAVAELTRGDVNQRL
                     EAAQRLRHAAEALVAELVAEARKKGATWSDIGDTLGVSTQAAHQKYRWVAARSRQS"
     gene            955..1028
                     /locus_tag="VLR27_17555"
     tRNA            955..1028
                     /locus_tag="VLR27_17555"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:987..989,aa:Gly,seq:ccc)
     gene            1391..>1894
                     /locus_tag="VLR27_17560"
     CDS             1391..>1894
                     /locus_tag="VLR27_17560"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HSP05316.1"
                     /translation="MTAPEHAARQADALPEVIGRAAVFDLDRTLLAGSSLVALARVMA
                     RHGMVSGQQLLSGLAMDQLFRRRGATDDDVDRLRTTALRQVAGTDVARLRALTDEVGR
                     TLSRQVLPGARMLLRRHQEAGDFIVILSASPQELVEAVAARLGAQRAVGTRAEIEGAR
                     YTGGLDGP"
BASE COUNT          260 a          633 c          707 g          294 t
ORIGIN      
        1 gtaggggacg tcggccgcgg tggtcatgcg caggaagctg acctggccga tcttcatctt
       61 cgggtagagc gtgatcggca ggttgtgcac gttggacagc tcgagggtga tctggccgta
      121 gaagcccggg tcgatgaacc cagctgtgga gtggatgagc aggccgaggc ggccgaggct
      181 ggacttgccg tcgaggcggg cgacgaggtc ctcggggacg ccgatgcgct cgagggtggc
      241 gccgaggacg aactcgcccg gatggaggac gaagggctgg tcgtcctcga ccgtgatcat
      301 ctcggtcagg tcgctggcgt cctccttcac gtcgatcatc ccgcgcgtgt ggttgcggaa
      361 cacgaggaac cgggggtcga gggtgaggtc gaccgacgag ggctggatga aggactcgtc
      421 caacgggtcg atgacgatgc gaccgttggc cagctcttcg cggatcgtgt ggtcggagag
      481 gatcacggcg ggcaggttag gtgcccgcgc ggcccggtca ggactggcgg gagcgggcgg
      541 cgacccagcg gtacttctgg tgcgccgcct gcgttgagac gccgagggtg tcaccgatgt
      601 cggaccaggt ggcgcccttc ttgcgggcct cggccaccag ctcggcgacg agggcctcgg
      661 cggcgtggcg gagtcgctgt gcggcctcga ggcgctggtt cacgtcacct cgggtgagct
      721 cggccacggc ctcgcgcatg gccgagtcgg ccgccaggtc gatctcgatg tcgccgcgcc
      781 cgacccgggc gatgatggtg ccctctcggc tctgttccac agcacaacgg tatgccagca
      841 ggtcgggtgt ggcggggatg cccggaaccg cccgtttcgg tgcggtcggg atgacctgca
      901 ccgacccgcc gtgcctagga tgggcgccct cagacggggc tccggcaccg tcctgcgggt
      961 gtagttcaat ggcagaacgt cagcttccca agctgagaac gcgggttcga ttcccgtcac
     1021 ccgctccata cgacagcccg accttcgtga cgaactccga ggtcatggcg cggagcagtg
     1081 cgctgtagtc gacgccccgc cggcgtccgc cgcgagggct cgggtgggtc ccaccgaacg
     1141 ttcaggcgcg aaggggcgtc ccgtcatccg tgggaaacct tccagccacg aggcacccgt
     1201 acggtcgggc agacaccggg tgaggagcag tcggcgctgc acccctggtg ggctcggtgc
     1261 acctctcccc agcggtgcac cgagccactt ctccgctcgg gctgctcgcg gccgggacct
     1321 tcacccatgg ttcacgccgt gtgccccgcc ggtctgcgcc gcggagaggt cgggatggtg
     1381 tcctgggacc atgaccgcac cggagcacgc tgcacgccag gcggatgccc tgcccgaggt
     1441 gatcggccga gccgccgtgt tcgacctcga tcggaccctg ctggcgggtt cgagcctggt
     1501 cgcgctggcc cgggtgatgg ccaggcacgg catggtctcg ggccagcagc tgctctcggg
     1561 gctggcgatg gaccagctct tccggcgtcg tggcgccacc gacgacgacg tcgaccgtct
     1621 ccgaaccact gcgctccgcc aggtcgccgg caccgacgtc gcgcggctgc gcgccctcac
     1681 cgacgaggtc ggccgcacgc tgagccgcca ggtcctcccc ggcgctcgca tgctgctgcg
     1741 gcgccaccag gaggccggcg acttcatcgt catcctgtcg gcgagcccgc aggagctggt
     1801 cgaggcggtc gcagcccggc tcggcgcgca gcgggcggtc ggcacccgcg ccgagatcga
     1861 gggcgcgcgc tacaccggcg gcctcgacgg accg
//
LOCUS       DATISU010000442         5017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_322606, whole genome shotgun sequence.
ACCESSION   DATISU010000442 DATISU010000000
VERSION     DATISU010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322606"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..348)
                     /locus_tag="VLR27_17565"
     CDS             complement(100..348)
                     /locus_tag="VLR27_17565"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSP05317.1"
                     /translation="MLQLHRWEADEHPEMGNPDDPSRGNGILLWFSTDDVAAVVERAR
                     SASADVIDGPLDNPNSHLREIWLRHPDGYVVVVAGPAT"
     gene            complement(370..1215)
                     /locus_tag="VLR27_17570"
     CDS             complement(370..1215)
                     /locus_tag="VLR27_17570"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP05318.1"
                     /translation="MTAPTTNGRTDPSLAAPDPAADHDWREAGSAWGHAAEDWSCLFE
                     HYATAAITAIHRRTEVGPGTTLLDVACGSGLALVHAADAGATVHGLDAAEPLVEIARD
                     RLPGVDVRLGSMYDLPWPAASFDVVTSINGVWGGCDAAVVEAHRVLRPGGWLAISFWG
                     DGEPLDLRPVFKVFARHAPETHFSGMKRLNDIAHHGVAEAMLADGGFVDIERHHRVSV
                     IEWPDPDIAWRALSSTGPAVPALEHVGHAALKEAVLRALEHCRDERGTYRFRNDHQIV
                     LGRRP"
     gene            1385..1948
                     /locus_tag="VLR27_17575"
     CDS             1385..1948
                     /locus_tag="VLR27_17575"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSP05319.1"
                     /translation="MTLTDQPSVDNGVNVQALLDARAALTDAPEAAAFQWRAQAQWVA
                     GTHTRTTVESYFGLGAEQSHRQAFVVDTDHPELFASEDNGMTPIEMVLTGLAGCLTAG
                     IASVATNRDVQLRSVTATITGDMDIQGILGIDPDVRNGFGGITVRYEIDADASRSDIE
                     AIVAQSQKRSAVFDIVANPTSIVVEVA"
     gene            1976..3196
                     /locus_tag="VLR27_17580"
     CDS             1976..3196
                     /locus_tag="VLR27_17580"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HSP05320.1"
                     /translation="MDTTAVVVIGAGPAGLAVSGALAAAGVDHVVLERHGVGHSWRHE
                     RWDTLRTLTPNWMNGLPGLPYRGPDPDGYMTAGELADLLDGYASSIAAPVVTGVEVSA
                     IRPRRRGHVVESDAGSWLADAVVLATGPGQSDVPAVSAHLPESLCQVTASHYRNPGQL
                     GERVLVVGASASGVQIADEVVRSGRDVTLAVGEHVRLPRTYRGRDILWWMQALGISSR
                     RWDDDLTDLARARHVPSPQLVAGRTLDLRTLREQGVELVGRLVGVRDRRLQLSGSLAN
                     VIANADLKQARLLDQIDEFIAEVGVAALAPERPPPTVVDAPPTSVDVAAFDTVLWATG
                     NRYAPPQLDACHLDRRGRVVHDGGLLRTPGLFAIGLPFLRRRSSTFLSGIGRDAVELT
                     IEIRRHLDRATAAA"
     gene            3215..4432
                     /locus_tag="VLR27_17585"
     CDS             3215..4432
                     /locus_tag="VLR27_17585"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSP05321.1"
                     /translation="MDTEGVLYRFGDTEVNCDTFEIVAGGSRVPVEPQVFEVIRFLVE
                     HRERMCTRSEILDAVWGDHFVSDSALASRISAARAAVGDDGRAQRVIRTVHGRGFQFV
                     APVVVDAPGRPPALVAEPDGQRRRQSIRFCQTPDGMHLAVAEVGTGPPLVKAATWMTH
                     VEHDWHSPVWRHWLDELGSRFRYIRYDARGGGLSDRDLTGSRLDELATWTSDLETAVD
                     CSEVERFVLFAMSQGVGPALDYCVQHPDRVSRLVVLGGYVRGMRRRDADAAAQAALIP
                     ELIRVGWGGSNPAFRTVFTTTFVPEATSEQMRWFNELSLQTASAENAVQLETAFHDQD
                     FSDLARAVRVPTLVLHGRDDRAVPFSEGRMLASLIPGAEFVPLDSANHVLLEHEPAWH
                     TFLSHLDRFTAGA"
     gene            4479..>5017
                     /locus_tag="VLR27_17590"
     CDS             4479..>5017
                     /locus_tag="VLR27_17590"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HSP05322.1"
                     /translation="MAITTEPRLGQDARIGVLFERLAELQGQRNVIDAQIVDVVRELE
                     QDDLWGITGCRSLESCVAWKTGSSYAHARSLAAVARRADAFPRCVGGLREGSLSLDQV
                     AVIAEKAIDGSDEHYADLAESATVSQLRTALNLAPRPDTEPPAEPERSVTKITDDEFA
                     IWRVKLPHVEAAVFDAALQS"
BASE COUNT          700 a         1757 c         1840 g          720 t
ORIGIN      
        1 gacgtctgcg gggcgggtct cgatcgtgtg tccctgggtc atgctccgtg caacgagcaa
       61 ccagggctgc ccattccgtg tgggtcggga ctgcgttgct caggttgcgg gcccggccac
      121 cacgacgacg tagccgtcgg ggtgccggag ccagatctcg cgcaggtggg agttggggtt
      181 gtcgagcgga ccgtcgatca cgtcggcgga cgccgagcgg gctcgctcga ccacggccgc
      241 gacgtcgtcg gtggagaacc acaggaggat gccgttgccg cgggacgggt cgtcggggtt
      301 gcccatctcc gggtgctcgt cggcctccca gcggtggagc tgcagcactg ccagttcctt
      361 cggggccggt caggggcgtc ggccgaggac gatctggtgg tcgttgcgga accggtacgt
      421 gccgcgctcg tcacggcagt gctccagcgc gcggagcacc gcctccttga gcgcggcatg
      481 tccgacgtgc tccagcgctg gcacggccgg gcccgtgctc gacagggcgc gccaggcgat
      541 gtcggggtcg ggccactcga tgaccgagac gcggtggtgt cgctcgatgt cgacgaagcc
      601 gccgtcggcg agcatcgcct cggcgacgcc gtggtgcgcg atgtcgttca agcgcttcat
      661 gccactgaag tgggtctccg gagcgtgacg tgcgaagacc ttgaacacgg ggcggaggtc
      721 gaggggttcg ccgtcgcccc agaagctgat cgccagccag ccgcccggcc ggagcacccg
      781 gtgcgcctcc acgaccgccg cgtcgcagcc accccacacg ccgttgatcg aggtcaccac
      841 gtcgaaggag gccgcaggcc aggggaggtc gtacatcgaa cccagccgga cgtcgacgcc
      901 cgggagtcgg tcgcgggcga tctccaccag cggttcggcc gcgtcgaggc catggacggt
      961 cgcgccggcg tcggccgcgt ggaccagggc gagcccggaa ccgcacgcga cgtcgagcag
     1021 cgtcgtgccc gggccgacct ccgtgcgtcg gtggatggcg gtgatcgccg cggtcgcgta
     1081 gtgctcgaac aggcaggacc agtcctccgc cgcatggccc caggcgctcc cggcttctcg
     1141 ccagtcatgg tcggcggcgg ggtccggcgc cgccagcgaa ggatcggttc ggccgttcgt
     1201 ggtgggcgca gtcatggcgt gagcgtgcgg caccaccggc agcgagtctt ggaggcttcg
     1261 tggagaaggt ctcaggagat ctccacgaga tcgcagcatc ctctccaagt cccggcgccg
     1321 gctcccgtcg acagtggcac gagcacccag ccgtgcgacc gcaagcccac gaggagaacc
     1381 gaccatgaca ctcaccgacc agccgagcgt cgacaacggc gtgaacgtcc aggccctgct
     1441 cgatgcccga gccgcgctca ccgatgcacc ggaggcggcc gcgttccagt ggcgggccca
     1501 ggcgcagtgg gtggccggca cccacacccg caccaccgtc gagagctact tcggactcgg
     1561 cgcggagcag tcacaccgcc aggcgttcgt cgtcgacacc gatcatcccg agctgttcgc
     1621 gtccgaggac aacggcatga cgccgatcga gatggtcctg accggcctcg ccggctgcct
     1681 caccgccggc atcgcctccg tggccacgaa ccgcgacgtg cagctccgct cggtcaccgc
     1741 caccatcacc ggcgacatgg acatccaggg catcctcggc atcgaccccg acgtccgcaa
     1801 cgggttcggc ggcatcacgg tgcggtacga gatcgacgcc gacgcatcac gaagcgacat
     1861 cgaggcgatc gtggcgcagt cccagaagcg gtcggcggtg ttcgacatcg tcgccaaccc
     1921 gacgagcatc gtcgtcgagg tggcctgagc catccggcgt cgaggggatc gagccatgga
     1981 cacgacggcg gtcgtcgtga tcggcgccgg gccggcgggt ctggcagtga gcggggcact
     2041 ggcagcggcc ggcgtcgatc acgtggtcct cgagcgccac ggggtcggcc actcgtggcg
     2101 gcacgagcgg tgggacacgc tgcgcaccct gaccccgaac tggatgaacg gtctcccggg
     2161 tctgccgtac cggggtccgg acccggacgg gtacatgacg gcgggcgagc tcgcggacct
     2221 gctcgacggt tacgccagct cgatcgcagc gccggtggtg accggggtcg aggtctccgc
     2281 catccgacct cgccggcgcg gccacgtcgt cgagtccgac gccgggagct ggctcgcgga
     2341 tgccgtggtg ctcgcgacag gaccgggcca gagcgacgtc ccggcggtct cggcccatct
     2401 gcccgagtcc ctgtgccagg tcacggcgtc gcactaccgg aaccccggcc agctcggtga
     2461 gcgggtgctc gtcgtggggg cgtcggcgtc gggcgtccag atcgccgacg aggtggtgcg
     2521 cagcgggcgc gacgtgacgc tggcggtggg cgagcacgtg cggctgcccc ggacctaccg
     2581 ggggcgggac atcctctggt ggatgcaggc gctggggatc agctcccggc gctgggacga
     2641 cgacctcacc gacctggccc gggcccggca cgtgccgtcc ccgcagctgg tcgcgggtcg
     2701 cacgctcgac ctgcgcacgc tgcgcgagca gggcgtggag ctcgtgggcc ggctggtcgg
     2761 cgtccgtgac cggcggctgc agctctccgg ttcgctggcc aacgtgatcg ccaacgccga
     2821 cctgaagcag gcccgcctgc tcgaccagat cgacgagttc atcgccgagg tcggcgtcgc
     2881 cgcactcgcg ccggagcggc cacccccgac cgtggtcgac gccccaccga cctccgtcga
     2941 cgtcgccgcc ttcgacacgg tgctctgggc gacgggcaac cggtacgcgc cgccacagct
     3001 cgacgcctgc cacctggacc ggcgaggccg tgtcgtccac gacggtgggc tgctgcggac
     3061 gccgggcctg ttcgcgatcg ggctgccgtt cctgcgccgg cgcagctcga cgttcctgag
     3121 cggcatcggg cgagacgccg tcgagctcac gatcgagatc cgccggcacc tcgaccgcgc
     3181 caccgcagca gcctgagcac cctcggcggt ccgggtggat actgagggtg tgctctaccg
     3241 gttcggagac acggaggtga actgcgacac cttcgagatc gtcgccggtg gatcgcgggt
     3301 cccggtcgag ccgcaggtgt tcgaggtgat ccgcttcctc gtcgagcacc gggagcggat
     3361 gtgcacccgg agcgagatcc tcgacgcggt ctggggtgac cacttcgtgt ccgacagcgc
     3421 gctggcgagt cggatctccg cggcgcgggc ggcggtgggc gacgacggcc gggcgcagcg
     3481 cgtgatccgc accgtgcacg gtcggggctt ccagttcgtc gccccggtcg tggtggatgc
     3541 gcccgggagg ccgccggcgc tggtcgccga gcccgatggg cagcgccggc gccagtcgat
     3601 ccgcttctgc cagacgcccg acggcatgca cctggcggtg gccgaggtcg ggaccggtcc
     3661 gccgctcgtc aaggcggcga cgtggatgac gcacgtggag cacgactggc acagcccggt
     3721 ctggaggcac tggctcgacg agctcggcag tcgcttccgg tacatccgct acgacgcgcg
     3781 gggcggcggg ctgtcggacc gcgacctcac ggggagtcgt ctggacgagc tcgccacgtg
     3841 gaccagcgac ctcgagaccg ccgtcgactg cagcgaggtc gagcggttcg tgctcttcgc
     3901 gatgtcacag ggggtggggc cggcgctgga ctactgcgtc cagcaccccg atcgggtctc
     3961 gcgcctggtg gtcctgggtg gctacgtccg cgggatgcgc cggcgcgatg cagacgccgc
     4021 ggcccaggcg gcgctgatcc ccgagctgat ccgcgtgggg tggggtgggt ccaaccccgc
     4081 cttccggacg gtgttcacga cgacgttcgt ccccgaggcg acgtcggagc agatgcggtg
     4141 gttcaacgag ctctccctgc agaccgcgtc cgccgagaac gccgtgcagc tcgagacggc
     4201 cttccacgac caggacttca gcgacctcgc ccgagcggtg cgagtgccca cgctggtgct
     4261 ccacggccgg gacgaccggg cagtgccgtt cagcgagggc cggatgctcg catcgctgat
     4321 ccccggcgcc gagttcgtgc cgctcgacag cgccaaccac gtcctgctcg agcacgagcc
     4381 ggcgtggcac accttcctct cgcacctcga ccggttcacc gccggcgcct gacccatttg
     4441 cggagttcct gtcagggggg tcgtgcgacg atcgctccat ggccatcacg accgaacccc
     4501 gactcgggca ggacgcccgg atcggcgtgc tgttcgagcg gttggccgag ctgcagggcc
     4561 agcgcaacgt catcgatgcc cagatcgtcg acgtggttcg cgagctcgag caggacgacc
     4621 tgtgggggat caccgggtgc cggtcgctgg agagctgcgt ggcgtggaag acggggtcgt
     4681 cgtacgcgca tgcgcgctcg ttggcagcgg tggctcgccg ggccgacgcc ttcccccgct
     4741 gcgtcggcgg gctgcgggag ggctcgctgt cgctcgacca ggtggcggtc atcgcggaga
     4801 aggcgatcga cggctccgac gagcactacg ccgacctcgc cgagtcagcc accgtgtccc
     4861 agctgcgcac cgcgctcaac ctggcgcccc ggccggacac cgagccgccg gccgagccgg
     4921 agcggtcggt caccaagatc accgacgacg agttcgccat ctggcgggtc aagctgcccc
     4981 acgtcgaggc tgcggtcttc gacgccgcgc tgcagtc
//
LOCUS       DATISU010000443        26991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_554878, whole genome shotgun sequence.
ACCESSION   DATISU010000443 DATISU010000000
VERSION     DATISU010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 26991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26991
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554878"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..498
                     /locus_tag="VLR27_17595"
     CDS             76..498
                     /locus_tag="VLR27_17595"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HSP05323.1"
                     /translation="MTVTHEGTADAGYEAATDEARHEHHPKDAQYWKVGLALGLITLL
                     EVGTYFIADPPYDHDLAPLLIGSLIFMMVLKFVVIGAYFMHLKFDNRLFRNVFVAGLL
                     LAVGVYLAVLTAFEFWTDRYEDGTYDEDTFLVEDAPAG"
     gene            complement(506..1342)
                     /locus_tag="VLR27_17600"
     CDS             complement(506..1342)
                     /locus_tag="VLR27_17600"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HSP05324.1"
                     /translation="MTVVGTVASIHRFPVKSMQGEQLTEVEIGPDGMVGDREWALRDI
                     ETGKLVSAKRPRPWRAALDCTATGLDDDVEIAMPSGEIFHVHDGGVAVALEALFGRSV
                     AIERSERRQQGTYDSEWPELDGITLSGDMELPTNLAGEGRGFIDLGVLHLLTTSSMAT
                     LQAEDDDLVVDVRRFRPSIVVDTPGFDGFPENDDWAGATLRIGRGDDAVEVTIGIPTP
                     RCIMTTVAQQDDLPRQPAVLQAIARLNRRTDELGSFACLGAYANVARPGTVRAGDEIE
                     LV"
     gene            1428..2351
                     /locus_tag="VLR27_17605"
     CDS             1428..2351
                     /locus_tag="VLR27_17605"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HSP05325.1"
                     /translation="MLFAAEDFFRWQPHPEIWVLVLGLVGLYTYAVRSLGPRAVPAGE
                     PVATPAQKRWFALGIVLLWIAADYPMHDVGEQYLYSVHMFQHMMLAFFIPPVMLLATP
                     EWLARLVIGDGNLGRWFLKLARPVPAAVVFNAVQLLTHWSVVVNASVENGLLHYALHT
                     VVVITAFAVWMPVVSPLPELRTTIPAQCVHLFLISIVPTVPAAWLALADGVLYDVYDQ
                     PARLWGISATHDQQLAGLIMKLGGGMFLWLIIIVLFFRWSTKQDRGTTARRAIVAEDG
                     SVTVEGPPALTYEEVAEAFERTSPPPREPAP"
     gene            complement(2358..3059)
                     /locus_tag="VLR27_17610"
     CDS             complement(2358..3059)
                     /locus_tag="VLR27_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05326.1"
                     /translation="MPARSEPRPVDTDGGGIGTRGESSLHAAVKLLVSEPGDRFEVPL
                     DGFVVDVVRGDTCIEVQTGGFAAMGNKLDRLLGDFHVHVVHPIAVESWIERRDKPTRK
                     SPKRGCLHDVFGELVSVPTMLDHPNLTIEVLLVQVDVLKVEDPTMRRRRGGWRTVDRR
                     LRAVLDRHGLRTPADLVALLPDGLPAEWTTKDVADGAGIPRRTAQQMAYVLKANGLAI
                     EVGRDRAGSRYVTAR"
     gene            3092..4189
                     /locus_tag="VLR27_17615"
     CDS             3092..4189
                     /locus_tag="VLR27_17615"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HSP05327.1"
                     /translation="MLLRLASEPLPSLSRARIYVCGITPYDTTHIGHAATFVWVDALT
                     RVLAHVGIEPEVVRNITDVDDELIREANERGTTWSALATQQTFQFEDDMRKLRVQRPA
                     FEPASRDYVTDVVFLARVLLDRGVAYERDGSVYFRAGDLWKRSGLEQAEAVELYGRRG
                     GRIDDPAKDDPADAAIWQRSAEGEPSWDSPWGRGRPGWHAECAAMATSILGLSIDVHA
                     GGADLAYPHHVYEQAIVEAATGVTPFARRWMHVGTVRRNGEKVAKSTGNLVFVMDLLQ
                     RWAPQVLRLHLLDRPWAEAWDFDEADLAASATRLEALWSAGSTPGGDEAAVRAALEAL
                     MDDLDVPRAIDIAIESGGEAARTVAKVLALL"
     gene            complement(4246..4989)
                     /locus_tag="VLR27_17620"
     CDS             complement(4246..4989)
                     /locus_tag="VLR27_17620"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSP05328.1"
                     /translation="MSALLAQLRTELTLTLRNKESLLLTLGIPIGLLVFFSLVDVLPL
                     DALLGAGDEPVDVLAPGVLALAIMSMAMTGLAIATGFERSYGVLKRLGTTPLGRPRLL
                     AAKIGAVVVVEAIQAAVLVPVALALGWEPSLAVVPLLGAVVLGTAAFAGLGLWMAGTL
                     RAEITLALANGLYLVLLLLGGFVIPLAELPDPLRRFAEALPSSALVDLVRAGFDGATG
                     PGRAWIVLIVWAVAMPLVAARTFRWSPAR"
     gene            complement(4986..5951)
                     /locus_tag="VLR27_17625"
     CDS             complement(4986..5951)
                     /locus_tag="VLR27_17625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP05329.1"
                     /translation="MSAVSVEHLVVRHGDLVAVDGVSFTAEAGEVLALLGPNGAGKTS
                     TVEVLEGYGRPDSGQVRVLGHYPGTERDRIVGRIGVMLQDGGVYPGIRPPEMLHLLAA
                     MYDDPADPDALLERVGLTDRAGSTWRQLSGGEQQRLSLAMALVGRPQVAFLDEPTAGV
                     DVRGRQVIRQIVRELADGGCAVVLCTHELDEAEKVADRVVIVDRGKVVATGTLDELRS
                     GTSGEVRFSAIPELDVDALGHHLGTTVTEVSPGEYVVADGGHDAHLAAKLTGWLANHD
                     VALGDLRAGRQRLEDVFLLLTADDAEPDVRRAMAPRRRGRRGRRS"
     gene            complement(5973..7355)
                     /locus_tag="VLR27_17630"
     CDS             complement(5973..7355)
                     /locus_tag="VLR27_17630"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HSP05330.1"
                     /translation="MHEDPAPARARAAWRTLTLAGAVLAALALGGGLAGAQEAEPEPG
                     VDPSAEEPVSTTQPPPPTTVVLPDGDFGVIPPGATGPGAPPPVEELPPDGLPPEAFPP
                     DEGELPVPEELPVVILPDPTPGLNAALAEIDVTAARRRLVAAALPAIVAEEHAVATRD
                     AMREAVATRRTATERAVAARTRLDALAVNALMYGDPAGLEPVDGPETLEHLRDRELRD
                     VVTDHVDRTFERAVEELRSAIQAEGDATAAHLAARRARHAAEQAHLLARADLERAIAQ
                     ASEARRQVGPAILGLPVLDSGDLVAWYRTYYAADPPFAPITAIVDAYLAIGTEEGVAA
                     DIAFAQAILETGGFTSGHARGFNFAGIGAYDHCAPTCGFRFPDLDSGVRAHVQLLRAY
                     ADPGLTSAQLAAPPNGLVAPERVGVRGCCQRWTGLTGVWATDPNYDRKVLGIYRLMVE
                     TARSLDRVQA"
     gene            7412..8008
                     /locus_tag="VLR27_17635"
     CDS             7412..8008
                     /locus_tag="VLR27_17635"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSP05331.1"
                     /translation="MGAQDLVVAAADYAREVHAADTRKGSGEPYFEGHLAPVAETVAA
                     HGGTPEQVAAAYLHDAAEDHGGQARLDDIRARFGDAVADIVADLSDSLVDTDAGEQKA
                     EWHVRKQAYLAAMRAKPDASLVVAAADKLHNATAIVEDHERVGDELWSRFTTRSGEDQ
                     RWYYESLATVLSERLPDHPTVVRLVDVVRRLGERLDAP"
     gene            complement(8085..9452)
                     /locus_tag="VLR27_17640"
     CDS             complement(8085..9452)
                     /locus_tag="VLR27_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05332.1"
                     /translation="MTEVQARPGGSDHVPPPALEDEPGHRWPSLTPTRLAVLSGMLYL
                     PFTTLGYGTDVDITNVLRSGESLLDGRYRYSRPPGALPYEGLVGVLDRIGGPTAVSVA
                     SALMAVVLLVLLGRLVSDELGERRGRIAVAVVATQPWFWLAATSLGDYVFALGLLVAG
                     ALAARHDRRVLAGLAFATAIAFRSSTAFLVWAFLLAELIGARGGLEGPDAVRPAPPTR
                     WRDALVTGVVTALAGLVWFIPSWLSVGRTGRFLVNEFETTGVVAMAGRWGVKNIAFFG
                     VFSLVVLAALAPVLLAALRLVRTSVLVRFAVLAAVVAELLFLRFPWKLAHLLPVLVCL
                     AILLAASPRLRSWHVGALIASQVLLAFVSVTVAEPDITNAATTGRVHLGVTWGVVVND
                     LDCRIDPPYGEGGWPDLDTPAADYAAIDMFTCQARPWRPGGIPDDYGRGASSGRPPPH
                     ELPEP"
     gene            9539..10504
                     /locus_tag="VLR27_17645"
     CDS             9539..10504
                     /locus_tag="VLR27_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSP05333.1"
                     /translation="MERFTDRIAVITGGGTGMGRELVRQLSAEGCHVALCDVNDDAMA
                     ETVALAEKEAPTGTRLTTFVADVADESQVQGFRDHVVAELDTDCVHLVFNNAGVGGGG
                     SFVLDDRAAWERTFGICWGGVYNGSRAFLPLLVASDGGHLVNVSSVNGLWASIGPFSP
                     HTAYSAAKFAVRGFTEALLTDLRINAPHVQAHVVMPGHIGTSIVLNSAAILGHRPEDM
                     TAEDLDRMRQTAARLGVDVSAVSDDELRGFMQVRGEAFRDGAPTTADQAATTILDGVR
                     AGRWRILVGDDAAVLDERVRADPERAYDAEFFDEMVAAGGMNLGL"
     gene            complement(10509..10931)
                     /locus_tag="VLR27_17650"
     CDS             complement(10509..10931)
                     /locus_tag="VLR27_17650"
                     /inference="COORDINATES: protein motif:HMM:NF014552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HSP05334.1"
                     /translation="MRIAFGTDEQTAVTDAVEAWLAEHGHDVVVVAERGGPWPGVGAA
                     VGRAVASGEADRGVVCCWTGTGVSIAANKVPGVRAALCTDRGTAEGARRWNDANVLAF
                     GLRLTTGAVVEELLDAFLATDVDPDERANIDAVEPPAT"
     gene            10971..11570
                     /locus_tag="VLR27_17655"
     CDS             10971..11570
                     /locus_tag="VLR27_17655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP05335.1"
                     /translation="MAVAVHATAADLPAVTEVLTAAFADDPVQQWLFAPADDPDAGRR
                     ALFEIFVEDYFWLGHAHVVADAGGIAGAAMWSPPDRDVLRGDRVQDLFGALTPHLGDA
                     LVPRLGELARTHEHRPAEPHLYLGILGVDPGRQSRGLGTSLLAPTLAECDRTGLLAHL
                     ESSNERNIPFYERHGFAVVDAYRCGGDGPLMTMMTRRPR"
     gene            11615..12397
                     /locus_tag="VLR27_17660"
     CDS             11615..12397
                     /locus_tag="VLR27_17660"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP05336.1"
                     /translation="MAHFDHDGIRIHYEDTGGDGLPILWSHGFLMDHTMFDPQVAGLD
                     GYRHIRWDERGFGDTDAAGPFSYWDSAEDAIALLDHLDIDRAVLAGMSQGGFLSLRAA
                     LAHPGRVRALILIDTQSGTEDPENVESYRGMIEAVSTGDEETRSGVFDIVGGLIIDDP
                     DLQPEWKAKWMAEDPARIVDPGGALLDRDDITERLGEITCPVLIVHGTADTAIPIEKA
                     ELLRAELPDCRDLVPIEGAAHAPNLTHPAEVNRHIERFLEAL"
     gene            12479..13915
                     /locus_tag="VLR27_17665"
     CDS             12479..13915
                     /locus_tag="VLR27_17665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP05337.1"
                     /translation="MTETTTTATGEVPDQPPATDSGTLAAPTAWGRMKALLSPGDGPP
                     LRQIAAAGGWYPLVIITSLNVVDELDRAVLGVFGPNVQRYFGMSDTTFGLLVGVQVTA
                     LVLLAVPIGYLGTKVDRGRILRWSALAWSIMSFATTFALKLPLFFLARFGVGIGKSGV
                     EPVGKSLLTDYYPPMAWNRVLAVHGAANPLGALIGPALAFGIAFFAGDGDDIWRWAFP
                     VLTVPSVIALIASRRLKEPENQMVRGLAGATLSVTGAPSGMNFREAVTRLLTIPTFKR
                     QILGIGVLGFGLVGVLAFVNIFLERQYGIGADGRAVIGMITATASLLGTLVGGNVGER
                     IFARSPARAVRIVGVAIAGFSMLLAFAVFLPTVWMFVPAAWVGLFALQVGIAPLYTAL
                     SAISPPRLRPLMFSLLGLMVAVMGGIAGGVLVGAIAEATDIRWGLAALAPTGLIGGLL
                     MARGATTIEADIARVAEESVGDPLAAFL"
     gene            13953..14312
                     /locus_tag="VLR27_17670"
     CDS             13953..14312
                     /locus_tag="VLR27_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05338.1"
                     /translation="MASSKYRVHDDGVAKARQMIDANQYDVDTEWSDAAPSADDGNDK
                     IDRDGWDGFGEWHLAIDTEANEETKDRYGFPYGDFRRVSRSGLIHAKQRASQNGHDEV
                     ERAAGELLDQLDRVRTD"
     gene            complement(14380..15546)
                     /locus_tag="VLR27_17675"
     CDS             complement(14380..15546)
                     /locus_tag="VLR27_17675"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSP05339.1"
                     /translation="MPVDLPRVRADTPGVDHVAHLNNAGAALPPRVVTDTVVEHLRRE
                     ATIGGYEAAAEAAERTAAVYASIARLLGASPPEIAVVDNATRAWDMVVYGFPFRRGDR
                     VLTARAEYASNAIALLQLARRHELEIVLVDDDEHGQIDLDHLDAELARGAALVSLTHV
                     PTSGGLVNPAAEVGARCREHGAFFMLDACQSAGQLPLDVTELGCDALSATGRKYLRGP
                     RGTGLLYVRDEWIERIEPPLLDLHSAEWTGPDRYEIRGDARRFETWECSHAARLGLGA
                     AVDHALEVGVAAGWERLRDLGEQLRRRLGELDRVSVHDKGRVRGGIVTFTVDGVPSAD
                     VQAALSRAAVNTSTTTPSHSVQDGRALPAMVRASAHYYNTEDELDRLLDVVAAL"
     gene            complement(15566..16792)
                     /locus_tag="VLR27_17680"
     CDS             complement(15566..16792)
                     /locus_tag="VLR27_17680"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HSP05340.1"
                     /translation="MLTPRTNHARFGLAALALLAPLQLLAIVRADEDRAQPAPVTASA
                     DERAAPEPTAPLVPPVAPAWMAAAAAPSGPSAATQLVTEQVAARTAPAPPPPAPPASG
                     ATFADRFPAHAAATQDPADPATTRWALLIGINEHRGVADNLGSRQDAEDLRTQLLARG
                     WRDDHILLLTDRQATRAGIVEGIAWLADKTDEDSVAVFHYSGHSKKWYGQDVDGDGEV
                     TDEGLWPSDNRFIVDSELVRLLDPVRPHAMWLSFATCNAAGFADPGLARPGRILTFSS
                     SEARKSYEHPSWGNSVWGSLVIDQAFGAGLGDLDGDGRVTVEEAFAWARPRAAETTSQ
                     QRYGAQDAVLMDDVDGDFDLRIPGAPPAPRQPAPDPDPAPPGADPAPPPPQREPEPSE
                     PTEEDHGNRICLLCGR"
     gene            complement(16895..18082)
                     /locus_tag="VLR27_17685"
     CDS             complement(16895..18082)
                     /locus_tag="VLR27_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05341.1"
                     /translation="MARDFGTTWWGEAWLDALEGIAGGRGGRLARGRRWAKANRGADL
                     HIEPGGVNAWVQADRDEPHEVHVGIRAFRDDEWDTVLDAIAAKAAHAAALLDGELDPA
                     VVRDAEAVEVDLLPRPRDLHTVCTCADDVEPCEHAAAACYLLADALDADPFVLLLLRG
                     RRREEVLDQVRRRRGGLPATAEDDETAPPAPEGIDAAEAWSRDLLALPLVPLVAHAPS
                     RPAAWPSDPPAHAPFDGAGLRTLVADAARRAWELCAGTVDTGLELDERADLARRVIGA
                     PPAVKQRLAEHSGVATVDLDVLGAAWQFGGAAGVRAHDEARWNPPPLEMARARDLMVE
                     AGMPIRRVHVSGNRVTGEGRLQLRRSRDGRWYLFTKATGRWAMAAPPEDDVDDLLDHD
                     LTS"
     gene            complement(18082..20856)
                     /locus_tag="VLR27_17690"
     CDS             complement(18082..20856)
                     /locus_tag="VLR27_17690"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSP05342.1"
                     /translation="MRLAPTTEATFVAGLHGRDGALALWDLDVDGDDAIDRTGEVTVV
                     LADGPRTLPADLVAVGDAVATLAALPAAAEVRPSVRAWSVATRLALDAVSRGRLLPAV
                     APDGADMWRLGPLDVADRTRVDQLADALPPAAHATPVADDATSPRLTSPNVATAGFLD
                     AVADSFVRTAAAAATVGFEAFAASHATDVTGAESWLQSTVAGTASDTAPGLRIALPDG
                     PDEPVRCVLQVQSRLDPSLVLDAAELWQAPAAVAARFGTDVETDLLLALRAAARVWAP
                     VERLLQEAAPEVLVLEDAEAEELFGPVADDLAAAGLPVLWPAEVLRPVELRPIIGTPA
                     PESGGGGAGLDLSALGEMRWHATIDGGDLTEAELLQLAEAKRPVVRLRGQWVRADPER
                     LRRLAERTPISTGDVLSAALSGTVTIGGETVEAEVDAPLRNLADRLRSIDTMRAHPAP
                     PELQATLRPYQERGLAWLKEMASLGLGGVLADDMGLGKTIQILALHLLRRGSGPTLIV
                     CPASLLGNWQREAARFCPDVPVRRYHGADRSLAGLADDELVVATYGIVRRDAAVLAEQ
                     PWDLLVADEAQAVKNPMSRTARQIRTIPAAARFALTGTPVENRLADLWALLDFTTPGL
                     LGKLDAFQQHVATPIERERDEEATARLAALVRPFLLRRRKTDPEIAPDLPPKTETDRF
                     VGLTTEQATLYKAVVTELLDDVAEAEGINRRGLVLKLLTALKQICNHPAQYLDQDSPI
                     TGRSGKLEAVTDLVETIASEGDSVLVFTQYVRMGHLLTRHLTALDLDVEFLHGGTPVP
                     GREAMVDRFQAGGPMVFVISLQAGGTGLNLTRATHVIHYDRWWNPAVEDQASDRAWRI
                     GQDRPVQVHRMICEGTLEERIATLLEAKRGLAESVVGGGESWVSELSDDELADLVELG
                     GGLDLGRFDR"
     gene            20906..22024
                     /locus_tag="VLR27_17695"
     CDS             20906..22024
                     /locus_tag="VLR27_17695"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSP05343.1"
                     /translation="MDPILTVPQGTFTLERAHHDPGEPLRAWDAADELALAHLAEVGA
                     AGERWLVVDDAHGALATALATHGPTSWTDSIVTMAATRANLERNGLDPSAVTSVPSTS
                     DPAGPVDVVVVKVPRTLAVLEDELHRLRPLLHAGSVVVGAGMTKAVHRSTIAAFEQAI
                     GPTPTSRARKKARLLLASVDPSLEPGPGPGPLRWVTPDGLEVTGLPGVFSASSLDPGT
                     RLLLDHLPAPEPGAVVVDLGCGTGVVGATVAHRHTDVSLVCCDESFRAVASARQTVGA
                     VASDATFHVTDVLDGVADASADLVLCNPPFHTGGARTTSVAYRMFADSARVLRPGGTI
                     TVVANRHLGHHVALRRWFDDVGVVGSDPRFVVLRGRRR"
     gene            complement(22028..22474)
                     /locus_tag="VLR27_17700"
     CDS             complement(22028..22474)
                     /locus_tag="VLR27_17700"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSP05344.1"
                     /translation="MSERPPFERTDLPRATRVVAAPVADVYAAVTEVTQWPSWMPELL
                     DPVIAKGDDRYLFRRQRNGRTDHNEGVVIVRGPTHTFGVQIDAAERVWFRTRPSPSGT
                     KVDLVLEPFGTATWRQRLRGRRRRNEQERWVHDVLDGLAGRAAPDA"
     gene            complement(22471..23115)
                     /locus_tag="VLR27_17705"
     CDS             complement(22471..23115)
                     /locus_tag="VLR27_17705"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HSP05345.1"
                     /translation="MDGGLREQKKAHTRQRLVQVALSLFEERGFDEVTVEEIADAAVV
                     SPRTFYRYFGSKEAVLYDGQDDQLILLHEVITSQPPEEPPLAAIRAAVLVLARQVAGT
                     AELSLRRARISASTTSLGEYQRSTLIPRWEQALTVAVAERLGVDPGSDPRPLLLAGIG
                     LTVMASVNETFQRVGAADLEAVVLARFADLTDLVADQGAAPTEQPVTEPRTGAG"
     gene            complement(23170..23631)
                     /locus_tag="VLR27_17710"
     CDS             complement(23170..23631)
                     /locus_tag="VLR27_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HSP05346.1"
                     /translation="MPTAARDLPVGRDELLDFVRPRHHLILGTTRQDGRVQLSPVTGG
                     IDAQGHVVVSSYPDRAKTANLRRDPRATALFLSDDFGDEWVQVHGTGEVLDLPDALEP
                     LCDYYRSISGEHPDWDEYREAMTRQGKVLLRITIDDWGPVSRGGFPPRLLD"
     gene            complement(23634..24014)
                     /locus_tag="VLR27_17715"
     CDS             complement(23634..24014)
                     /locus_tag="VLR27_17715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSP05347.1"
                     /translation="MADSEKPHVYVPPGEIPPIKLMIEDLEEGDGPTAASGDTVDVHY
                     VGVSWKTRQQFDASWDRGEPFSFRLGAGMVISGWDEGVQGMKVGGRRRLVLPPDKAYG
                     KQGAGGVIGPNETLVFVVDLLGVR"
     gene            24121..24432
                     /locus_tag="VLR27_17720"
     CDS             24121..24432
                     /locus_tag="VLR27_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009794840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSP05348.1"
                     /translation="MDVGPNLWRTTPMNKSELIEKLAADTDLPKSQVSAVVSAFEDTI
                     VAEVKSGNKVSLPGFLTFEKGHRAARTGKNPQTGEALQIAAADVPKVKVGKSFKDAVN
                     S"
     gene            24700..26508
                     /locus_tag="VLR27_17725"
     CDS             24700..26508
                     /locus_tag="VLR27_17725"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HSP05349.1"
                     /translation="MAAIAALTVGIFAVSPAGAAEPPGAVTTSDFCANAGDASMFEDI
                     DDSTFTDEIVCAELLGMTQGTTLTTYDPDADVTRGQMASFIARMIDKASELELGEVAE
                     LPAADPDAASFSDVDDESPHADAIARLAAVDIVLGGPGALPATAYGPQLPIDRDQMAS
                     LINRAVGFMTGSPLATDQEYFTDVSPENVHAANVNALAAAGITQGIGNEMYAPDQAVT
                     RGQMAAFLVRSFAVLAEAGVIAPPPPAANAAFAATLGELNGSGADGTATVVVEGTVAT
                     VRVDIAGASPDLVHAQHIHIGGNNECPTPANDTDGDGFISTPEGVPSYGGIAVSLTTE
                     GDTNPASGLAIARMPVASASGDVTYERTFRLPTGLTAADVANGVIVSHGVADTTLGTD
                     DSAYDGRDSPLAEGVPQEATLPAACGALDAPAEATSFQTALTELNGSTATGTATVLVN
                     GNLATVTVDIAGASPGLVHAQHIHIGGNNVCPTPADDTNGDGFISTPEGVPSYGGIAV
                     SLTTEGDTSPASGLAIARMPVASDASVVSYERSFVLPAGVTAADVEDGVIVSHGVAAT
                     TLGTDPATYDGPDSPLAEGVPQEATLPAACGALVAT"
     gene            complement(26688..>26991)
                     /locus_tag="VLR27_17730"
     CDS             complement(26688..>26991)
                     /locus_tag="VLR27_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HSP05350.1"
                     /translation="VWVDAAPPEGHVILNTGIMLERMSNGIIPSGWHRVVADPDHDGE
                     RYSVVQFCHPTPWTILAPLPSTVTPDTPARYAGIEAADLLDQVLYEINLVEDARRV"
BASE COUNT         3616 a         9881 c         9918 g         3576 t
ORIGIN      
        1 tcgtcgacat cgtgtgggtc ctgatcttca cgatcgtcta cctcatcccg tgaagccacg
       61 gaggagcacg caccgatgac cgtcacccac gagggcacgg ccgacgccgg ctacgaggcc
      121 gccacggacg aggcgcgcca cgagcaccac ccgaaggacg cccagtactg gaaggtcggg
      181 ctcgcgctcg gcctgatcac gctgctcgag gtcggcacgt acttcatcgc cgacccgccc
      241 tacgaccacg atctggcgcc cctgctcatc ggcagcctca tcttcatgat ggtgctgaag
      301 ttcgtcgtga tcggcgccta cttcatgcac ctgaagttcg acaaccgcct gttccgcaac
      361 gtcttcgtcg ccgggctgct gctggcggtc ggcgtgtacc tggccgtgct caccgccttc
      421 gagttctgga ccgatcgcta cgaggacggc acctacgacg aggacacgtt cctcgtcgag
      481 gacgcccccg ccggctgacg ccccgtcaca ccagctcgat ctcgtcgccc gcccgcaccg
      541 tgccggggcg ggcgacgttc gcgtaggcgc cgaggcaggc gaaggagccg agctcgtcgg
      601 tgcgccggtt gaggcgggcg atggcctgga gcacggcggg ctggcgaggc aggtcgtcct
      661 gctgggccac ggtggtcatg atgcagcggg gcgtggggat cccgatcgtg acctccaccg
      721 cgtcgtcgcc ccgaccgatc cgcagcgtgg cgcccgccca gtcgtcgttc tccgggaacc
      781 cgtcgaagcc cggggtgtcg accacgatgc tgggccggaa ccggcggacg tcgaccacga
      841 ggtcgtcgtc ctcggcctgc agcgtggcca tcgaggacgt ggtcagcagg tgcagcacgc
      901 cgaggtcgat gaagccgcgg ccctcgccgg ccaggttggt cggcagctcc atgtcccccg
      961 agagcgtgat gccgtcgagc tcgggccact ccgagtcgta ggtcccctgc tggcgccgct
     1021 cggaccgctc gatggccacg ctgcggccga acagcgcctc gagggcgacg gcgacgcccc
     1081 cgtcgtggac gtggaagatc tcgcccgagg gcatggcgat ctcgacgtcg tcgtcgagcc
     1141 cggtggcggt gcagtccagc gcggcccgcc acgggcgggg ccgcttggcg ctgaccagct
     1201 tgccggtctc gatgtcgcgc agcgcccact cccggtcgcc gaccatgccg tcggggccga
     1261 tctcgacctc ggtcagctgc tcgccctgca tggacttcac ggggaagcgg tggatggagg
     1321 cgacggtgcc gacgacggtc atggtcggcg agtctggccg accggcgtgc cccctgccac
     1381 cggtggccga ctgtccccgc tcggggggtg acccgtaggc tcaggccgtg ctcttcgccg
     1441 ccgaggactt cttccgctgg cagccgcacc ccgagatctg ggtgctggtc ctcgggctcg
     1501 tcgggctcta cacctacgcc gtccgcagcc tcggccctcg cgccgtgccc gccggcgagc
     1561 cggtggccac ccccgcccag aagcgctggt tcgccctcgg catcgtcctg ctgtggatcg
     1621 ccgccgacta ccccatgcac gacgtggggg agcagtacct gtactccgtc cacatgttcc
     1681 agcacatgat gctggccttc ttcatcccgc cggtgatgct gctcgccacc cccgagtggc
     1741 tggcccgcct cgtgatcggc gacggcaacc tgggccgctg gttcctcaag ctggcccgac
     1801 ccgtccccgc cgccgtcgtg ttcaacgcgg tgcagctgct cacccactgg agcgtcgtcg
     1861 tcaacgcgag cgtcgagaac ggcctcctcc actacgccct gcacaccgtc gtggtgatca
     1921 ccgcgttcgc ggtctggatg ccggtggtgt cgccgctgcc ggagctgcgc accaccatcc
     1981 cggcccagtg cgtccacctg ttcctgatct cgatcgtccc gacggtcccg gctgcctggc
     2041 tcgccctcgc cgacggcgtc ctctacgacg tctacgacca gcccgctcgg ctctggggca
     2101 tctctgccac ccacgaccag cagctcgccg gcctcatcat gaagctcggt gggggcatgt
     2161 tcctctggct catcatcatc gtgctgttct tccggtggag cacgaagcag gaccggggca
     2221 cgacggcccg gcgtgcgatc gtggccgagg acggctccgt caccgtcgag ggcccgccgg
     2281 cgctcaccta cgaagaggtc gccgaggcct tcgagcgcac gtcgccgcca ccccgcgaac
     2341 ccgccccgta gcggcgctca gcgggccgtc acgtagcggg agccggcccg gtcccgcccg
     2401 acctcgatcg ccaggccgtt cgccttgagc acgtaggcca tctgctgcgc ggtgcggcgg
     2461 gggatgccgg cgccgtcggc cacgtccttg gtggtccact cggcgggcag gccgtcgggc
     2521 agcagcgcca cgaggtcggc tggtgtccgg aggccgtggc ggtccagcac ggcccgcagg
     2581 cggcggtcca ccgtgcgcca cccaccacga cgccggcgca tggtggggtc ctccaccttg
     2641 agcacgtcga cctggacgag cagcacctcg atggtgaggt tggggtggtc gagcatggtc
     2701 ggcaccgaca ccagctcgcc gaagacgtcg tgcaggcagc cccgcttggg ggacttgcgg
     2761 gtcggcttgt cccgccgctc gatccaggac tccaccgcga tggggtgcac gacgtggacg
     2821 tggaagtccc cgaggaggcg gtcgagcttg ttgcccatgg cggcgaaccc gccggtctgc
     2881 acctcgatgc aggtgtcgcc ccgcaccacg tccacgacga agccgtcgag gggcacctcg
     2941 aagcggtcgc ccggctcgct caccagcagc ttcaccgccg cgtgcagcga gctctcgccc
     3001 cgggtgccga tgccgccgcc gtcggtgtcg accggacgcg gttcggagcg ggcaggcacg
     3061 ccatgagtct gccggaacgt agcctgcgat cgtgctcctg cgactcgcct ccgaaccgct
     3121 gccctcgctg tcgagagccc gcatctacgt ctgcggcatc acgccctacg acacgaccca
     3181 catcggccac gccgccacct tcgtgtgggt cgacgcgctg acccgcgtgc tcgcccacgt
     3241 cggcatcgag cccgaggtcg tgcgcaacat caccgacgtc gacgacgagc tgatccgcga
     3301 ggccaacgag cggggcacga cgtggtcggc gctggccacc cagcagacgt tccagttcga
     3361 ggacgacatg cgcaagctgc gggtgcagcg cccggcgttc gagccggcgt cacgggacta
     3421 cgtgaccgac gtcgtgttcc tggcccgcgt cctgctcgac cggggcgtgg cctacgagcg
     3481 cgacggctcg gtgtacttcc gggccggcga cctgtggaag cggtcgggcc tcgagcaggc
     3541 cgaggccgtc gagctctacg gccgccgggg cggtcggatc gacgacccgg ccaaggacga
     3601 ccccgccgac gccgccatct ggcagcgctc cgccgagggc gagccatcgt gggactcgcc
     3661 gtggggccgg ggccgccccg gctggcacgc cgagtgcgcc gcgatggcca cctccatcct
     3721 cgggctgtcg atcgacgtcc acgccggcgg cgccgacctc gcctacccgc accacgtcta
     3781 cgagcaggcc atcgtcgagg cggccaccgg ggtcacgccc ttcgcccggc gctggatgca
     3841 cgtcggcacc gtccgccgca acggcgagaa ggtggccaag tccaccggca acctcgtctt
     3901 cgtgatggac ctgctgcagc gctgggcacc ccaggtgctg cgcctccacc tgctcgaccg
     3961 cccctgggcc gaggcgtggg acttcgacga ggccgacctc gccgccagcg ccacccgcct
     4021 cgaggccctg tggtccgccg gcagcacccc aggcggcgac gaggccgcgg tgcgcgccgc
     4081 gctcgaggcc ctcatggacg acctcgacgt gccccgcgcc atcgacatcg ccatcgagtc
     4141 cgggggcgag gccgcccgca cggtcgccaa ggtcctggcg ctcctctgat ccgagaactg
     4201 gcagtgctgc tgtagcgcgc tacagctcga ctgccagttc tcgggtcagc gggctgggga
     4261 ccagcggaac gtccgggcgg cgaccagcgg catggcgacg gcccacacga tcagcacgat
     4321 ccaggcccgg ccggggccgg tggcgccgtc gaatccggcc cgcaccaggt cgacgagggc
     4381 cgacgacggc agggcttcgg cgaagcgacg gagcgggtcg ggcagctcgg cgagcgggat
     4441 cacgaacccg ccgagcagca gcagcaccag gtagagcccg ttggccaggg ccagggtgat
     4501 ctcggcgcgg agcgtgccgg ccatccacag gccgaggccg gcgaacgcgg ccgtcccgag
     4561 cacgaccgcg ccgagcaggg gcacgacggc gagcgacggc tcccagccga gggcgagcgc
     4621 cacgggcacc agcacggcgg cctggatcgc ctccaccacg acgaccgcgc cgatcttggc
     4681 ggcgagcagc cggggccggc cgaggggcgt cgtgccgagg cgcttcagca cgccgtagga
     4741 ccgctcgaag ccagtggcga tggcgaggcc ggtcatggcc atcgacatga tcgccagggc
     4801 gaggacgccg ggggccagca cgtcgaccgg ctcgtcgccg gcgccgagga gggcgtcgag
     4861 cggcaggacg tcgacgaggg agaagaagac cagcagcccg atcgggatgc cgagcgtcag
     4921 cagcagggac tccttgttgc gcagcgtgag ggtcagctcg gtgcgcagct gggcgaggag
     4981 ggcgctcacg agcgccgtcc gcggcggccg cgccgcctcg gcgccatggc ccgccgcacg
     5041 tcgggttcgg cgtcgtcggc ggtgagcagg aggaacacgt cctcgagccg ctgccgcccg
     5101 gcccggaggt cgccgagggc gacgtcgtgg ttggccagcc agccggtgag cttggcggcc
     5161 aggtgggcgt cgtggccgcc gtcggcgacg acgtactcgc ccggggagac ctcggtgacg
     5221 gtggtgccca ggtggtggcc gagggcgtcg acgtccagct cggggatggc cgagaagcgc
     5281 acctcgccgc tcgtgccgga ccgcagctcg tcgagggtgc cggtggccac gaccttgccc
     5341 cggtcgacga tcacgacccg gtcggccacc ttctcggcct cgtccagctc gtgggtgcac
     5401 agcacgacgg cgcacccgcc gtcggccagc tcccggacga tctgacggat gacctgccgg
     5461 ccccgcacgt cgacgccggc ggtgggctca tcgaggaacg cgacctgtgg gcgtccgacg
     5521 agcgccatgg ccaggctgag ccgctgctgc tcaccgccgg acagctggcg ccacgtggac
     5581 ccggcccggt cggtgaggcc gacccgctcg agcagcgcat cggggtcggc ggggtcgtcg
     5641 tacatggcgg cgagcaggtg gagcatctcc ggtgggcgga tgccggggta gacgccgccg
     5701 tcctggagca tgacgccgat gcgcccgacg atccggtcgc gctcggtgcc ggggtagtgg
     5761 ccgagcaccc gcacctgccc gctgtcgggt cgtccgtagc cctccagcac ctcgaccgtg
     5821 ctagtcttgc cggcaccgtt cggtccgagc agggccagca cctcgccggc ctcggccgtg
     5881 aagctcacgc cgtccaccgc caccaggtcg ccgtggcgga cgacgaggtg ctcgacgctg
     5941 acggcggaca tcccggacac gctagggact tgtcaggcct ggacgcgatc gagcgaccgg
     6001 gccgtctcca ccatcaggcg gtagatgccg agcaccttgc ggtcgtagtt cggatcggtg
     6061 gcccacacgc ccgtgagacc cgtccagcgt tgacagcagc cccgcacgcc gacccgctcc
     6121 ggtgcgacca gcccgttcgg cggcgcggcc agctgtgccg acgtcaaccc ggggtcggcg
     6181 taggcccgca gcagctggac gtgggcgcgc acgcccgagt cgaggtcggg gaaccggaag
     6241 ccgcacgtcg gggcgcagtg gtcgtacgcc ccgatcccgg cgaagttgaa gccccgggcg
     6301 tgaccggagg tgaacccacc ggtctcgagg atcgcctggg cgaaggcgat gtcggcggcg
     6361 acgccctcct cggtgccgat ggccaggtag gcgtcgacga tcgcggtgat cggggcgaag
     6421 ggcgggtcgg cggcgtagta ggtgcggtac caggccacga ggtcaccgga gtcgagcacg
     6481 gggaggccga ggatggccgg accgacctgg cgccgcgctt cgctcgcctg ggcgatggcg
     6541 cgctcgaggt cggcccgggc gaggaggtgt gcctgctccg ctgcgtgccg ggcgcgacgg
     6601 gcggcgaggt gggcggccgt ggcgtctccc tcggcctgga tcgccgagcg cagctcctcg
     6661 acggcgcgtt cgaaggtgcg gtcgacgtga tcggtcacca cgtcccggag ctcgcggtcg
     6721 cggaggtgct cgagcgtctc ggggccgtcg acgggctcca agccggcggg gtcgccgtac
     6781 atgagggcgt tcaccgccag ggcgtcgagg cgcgtccgcg ccgcgaccgc ccgctccgtc
     6841 gccgtcctcc tggtcgcgac ggcctcgcgc atcgcgtcgc gggtggccac cgcgtgctcc
     6901 tccgccacga tggcgggcag ggcggccgcc accaggcgac ggcgggcggc ggtgacgtcg
     6961 atctcggcga gggcggcgtt cagccccggg gtcgggtcgg gcaggatcac caccggcagc
     7021 tcctcgggga cgggcagctc gccctcgtcc ggggggaacg cctcgggcgg gaggccgtcc
     7081 ggcgggagct cctccacggg cggcggggcg ccgggcccgg tggcgccggg tgggatcacc
     7141 ccgaagtcgc cgtcggggag cacgacggtg gtcggcggag gcggctgcgt ggtcgagacg
     7201 ggttcctcgg cggacgggtc gacgccgggc tccggctcgg cctcctgggc gccggcgagg
     7261 ccgccgccga gcgcgagcgc ggcgaggacg gcgccggcca gcgtcagggt gcgccaggcc
     7321 gcgcgggcgc gcgccggcgc agggtcctcg tgcacgggcg cagggtacgt ggggggtgac
     7381 gggaccgggg ggagcgtggc aacgttgcgc cgtgggagca caggacctgg tggtggcggc
     7441 cgcggactac gcacgggagg tccacgccgc cgacacccgc aagggcagcg gcgagccgta
     7501 cttcgagggg cacctcgccc cggtcgccga gaccgtcgcc gcccacggcg ggacgccgga
     7561 gcaggtcgcg gcggcctacc tccacgatgc cgccgaggat cacggtggcc aggcccgcct
     7621 cgacgacatc cgtgcccgct tcggcgacgc cgtggccgac atcgtcgccg acctgtccga
     7681 ctcgctggtc gacaccgacg ccggcgagca gaaggccgag tggcacgtgc gcaagcaggc
     7741 ctacctggcc gccatgcggg ccaagccgga cgcgtcgctc gtcgtggcgg ccgccgacaa
     7801 gctgcacaac gccaccgcca tcgtcgagga ccacgagcgc gtcggcgacg agctgtggtc
     7861 ccgcttcacc acccggagcg gcgaggacca gcgctggtac tacgagtcgc tggcgacggt
     7921 gctgtcggag cgtctgcccg accacccgac ggtcgtgcgc ctggtcgacg tggtccgtcg
     7981 cctcggcgag cgcctcgacg ccccctgagg gcaggaccgt tccgggccgc ggtgcacgca
     8041 ccgggggcgt ggggagcggc ctcgaaacga acggtgctcg gccgtcaggg ctcgggcagc
     8101 tcgtgcggtg gcggccggcc cgacgaggcg ccccggccgt agtcgtcggg gatgccgccg
     8161 ggccgccacg gccgggcctg gcaggtgaac atgtcgatgg cggcgtagtc ggcggccggc
     8221 gtgtcgaggt ccggccaccc gccttcgccg tagggcgggt cgatgcggca gtcgaggtcg
     8281 ttgaccacca cgccccacgt cacgccgagg tggacccggc cggtggtggc ggcgttggtg
     8341 atgtcgggct cggccacggt gacggacacg aacgcgagca gcacctggga ggcgatcagc
     8401 gccccgacgt gccaggagcg gaggcgcggg cttgcggcca gcaggatcgc caggcacacc
     8461 agcaccggca gcaggtgcgc cagcttccag gggaagcgca ggaacagcag ctcggcgacc
     8521 accgccgcca ggacggcgaa gcgcacgagc acgctcgtgc ggaccagccg cagggcagcg
     8581 agcagcaccg gagcgagcgc ggcgaggacg acgagggaga acaccccgaa gaaggcgatg
     8641 ttcttgacgc cccagcggcc cgccatggcg acgacgccgg tggtctcgaa ctcgttgacc
     8701 aggaagcgcc cggtgcgtcc gaccgacagc cacgacggga tgaaccagac caggccggcg
     8761 agggcggtca ccacccccgt cacgagggcg tcccgccagc gtgtgggtgg cgccgggcgg
     8821 acggcgtcgg gcccctcgag cccgccgcgg gcgccgatga gctcggcgag caggaaggcc
     8881 cagaccaaga acgccgtgct ggaccggaag gcgatggcgg tggcgaacgc cagcccggcg
     8941 aggacgcggc ggtcgtggcg ggcggcgagc gcgccggcca ccagcaggcc gagggcgaac
     9001 acgtagtcgc cgagcgaggt ggcggcgagc cagaaccacg gttgggtcgc caccacggcc
     9061 acggcgatgc gacctcggcg ctcgccgagc tcgtcggaga ccagccgtcc gagcaggacc
     9121 agcaggacga cggccatgag tgccgacgcc acgctcacgg cggtgggccc gccgatgcgg
     9181 tcgagcacgc cgaccaggcc ctcgtagggg agcgcgcccg gtgggcggga gtaccggtag
     9241 cggccgtcga gcagggactc gccgctgcgg agcacgttgg tgatgtcgac gtcggtgccg
     9301 tagcccaacg tggtgaacgg caggtagagc atgccgctca gcacggcgag ccgggtcggg
     9361 gtgagggacg gccagcggtg gccgggctcg tcctcgaggg ccggtggcgg cacgtggtcc
     9421 gacccgccgg gccgtgcctg cacctcggtc atcgccaggc acggtaccgg gccagtaccc
     9481 ttgaccgccc ggtcaagtcc gcccgacggg cggcggcacg acgtccgtag ggggacacat
     9541 ggagcgcttc accgatcgca tcgccgtcat caccggtggt ggcaccggca tgggacggga
     9601 gctggtccgc cagctgtcgg ccgagggctg ccacgtggcg ctctgcgacg tgaacgacga
     9661 cgccatggcc gagacggtcg cgctggccga gaaggaggcg cccaccggca cccggctcac
     9721 gaccttcgtc gccgacgtcg ccgacgagtc ccaggtgcag ggcttccgcg accacgtggt
     9781 ggccgagctc gacaccgact gtgtccacct ggtgttcaac aacgccggcg tgggcggcgg
     9841 cggctccttc gtcctggacg accgggcggc gtgggagcgc accttcggca tctgctgggg
     9901 cggggtctac aacggctccc gcgccttcct gcccctgctc gtcgcctcgg acggcggcca
     9961 cctcgtcaac gtctccagcg tcaacgggct ctgggcgtcg atcggtccgt tctcgcccca
    10021 caccgcctac agcgccgcca agttcgcggt gcgcggcttc accgaggcgc tgctcaccga
    10081 cctgcggatc aacgcaccgc acgtccaggc ccacgtggtc atgcccggcc acatcggcac
    10141 ctcgatcgtg ctcaactccg ccgccatcct cggccaccgg cccgaggaca tgaccgccga
    10201 ggacctcgac cgcatgcgcc agaccgccgc ccggctcggc gtggacgtga gcgcggtcag
    10261 cgatgacgag ctgcgggggt tcatgcaggt gcggggcgag gcgttccgcg acggcgcgcc
    10321 gaccaccgcc gaccaggccg ccaccaccat cctcgacggc gtgcgggccg gccgctggcg
    10381 catcctcgtg ggcgacgacg ccgccgtgct cgacgagcgg gtgcgcgccg atcccgagcg
    10441 ggcctacgac gccgagttct tcgacgagat ggtcgccgcc ggcgggatga acctgggcct
    10501 gtagagcgtc aggtcgcggg cggctcgacg gcgtcgatgt tcgcccgctc gtcggggtcg
    10561 acgtcggtgg cgaggaacgc gtcgagcagc tcctcgacca cggctccggt ggtgagccgc
    10621 agcccgaagg cgagcacgtt ggcgtcgttc caccggcgag cgccctcggc cgtgccgcgg
    10681 tccgtgcaga gcgcggcccg gaccccgggc accttgttgg cggcgatgct cacgccggtg
    10741 ccggtccagc agcacaccac gccccggtcg gcctccccgg acgccacggc ccgaccgacg
    10801 gctgcgccga cgcccggcca cgggccacca cgctcggcca ccaccacgac gtcgtggccg
    10861 tgctcggcca gccaggcctc gaccgcgtcg gtgacggcgg tctgctcgtc ggtgccgaag
    10921 gcgatgcgca cgtcgcgacc ctaggacgac ggtccaccta cgctccggcg atggccgtcg
    10981 ccgtgcacgc caccgccgcc gacctgccgg ccgtgaccga ggtgctcacc gccgcgttcg
    11041 ccgacgaccc ggtccagcag tggctcttcg cacctgccga cgatcccgat gccggccggc
    11101 gcgccctgtt cgagatcttc gtcgaggact acttctggct cggccacgcc cacgtcgtcg
    11161 ccgacgccgg tggcatcgcg ggcgcggcga tgtggtcgcc acccgaccgc gacgtgctcc
    11221 gcggcgaccg ggtgcaggac ctgttcggcg cgctcacccc ccacctcggc gacgccctgg
    11281 tcccccgcct cggcgagctg gcccggaccc acgagcaccg gccggcggag ccccacctct
    11341 acctcggcat cctcggcgtc gaccccggtc gccagtcccg gggcctcggc acgtccctgc
    11401 tggccccgac cctggccgag tgcgaccgga ccggtctgct cgcccacctc gagtcgtcca
    11461 acgagcggaa catcccgttc tacgagcgcc acgggttcgc cgtggtcgac gcctaccggt
    11521 gcggtggcga cggcccgctc atgacgatga tgacccgccg gccccgctga ccgaccgccg
    11581 agcgcgccct cccgggcggg gctaccgtcc gcgcatggcg cacttcgacc acgacgggat
    11641 ccggatccac tacgaggaca ccggcggcga cggcctgccg atcctctgga gccacgggtt
    11701 cctcatggac cacacgatgt tcgacccgca ggtcgccggc ctcgacgggt accgccacat
    11761 ccgctgggac gagcgtggct tcggcgacac ggacgcggcc ggcccgttca gctactggga
    11821 ctcggccgag gacgcgatcg ccctgctcga ccacctcgac atcgaccgtg ccgtgctggc
    11881 cggcatgtcc cagggcggct tcctgtcgct gcgcgccgcc ctcgcccacc ccggtcgggt
    11941 ccgggcgctg atcctcatcg acacccagtc cggcaccgag gaccccgaga acgtggagtc
    12001 ctaccggggc atgatcgagg cggtgtcgac cggcgacgag gagacccgca gcggggtctt
    12061 cgacatcgtc ggcggtctca tcatcgacga ccccgacctg cagccggagt ggaaggcgaa
    12121 gtggatggcc gaggacccgg cccgcatcgt cgatcccggc ggtgccctgc tcgaccgcga
    12181 cgacatcacc gaacggctcg gggagatcac ctgtcccgtg ctcatcgtgc acggcacggc
    12241 ggacaccgcc atcccgatcg agaaggccga gctgctgcgg gccgagctcc cggactgccg
    12301 cgacctggtg ccgatcgagg gggcggccca cgccccgaac ctcacccacc cggccgaggt
    12361 gaaccgccac atcgagcgct tcctcgaggc gctgtagcga cgtcggtcac gtcagcagga
    12421 aacgcctgta gccacgacga agcacgggcg gaccggcgaa cggcagtggg ggcagcaggt
    12481 gaccgagacg acgacgaccg cgacgggcga ggtcccggac cagccgcctg cgacggactc
    12541 cgggacgctc gccgccccca ccgcctgggg ccggatgaag gccctgctct cacccggcga
    12601 cgggccaccg ctgcgccaga tcgccgcggc cggcggctgg tacccgctgg tcatcatcac
    12661 ctcgctcaac gtcgtcgacg agctcgaccg ggcggtgctc ggcgtgttcg gccccaacgt
    12721 gcagcgctac ttcggcatga gcgacaccac gttcggcctg ctggtcgggg tgcaggtcac
    12781 ggccctggtg ctgctcgccg tgcccatcgg ctacctgggc accaaggtcg accgcggccg
    12841 gatcctgcgc tggtcggccc tggcctggtc gatcatgagc ttcgccacca cgttcgccct
    12901 caagctgccg ctgttcttcc tggcccgctt cggggtgggc atcggcaagt cgggcgtgga
    12961 gccggtgggc aagtcgctgc tcaccgacta ctacccgccg atggcctgga accgggtgct
    13021 ggccgtccac ggggcggcca accccctcgg tgcgctgatc ggccccgcgc tcgccttcgg
    13081 catcgccttc ttcgccggcg acggtgacga catctggcgc tgggcgttcc cggtgctcac
    13141 cgtgccctcg gtgatcgcgc tcatcgccag ccggcgtttg aaggagccgg agaaccagat
    13201 ggtccgcggc ctggccggcg ccaccctgtc ggtcaccggt gcgccgtcgg gcatgaactt
    13261 ccgcgaggcc gtcacccggc tgctcaccat ccccacgttc aagcgccaga tcctcggcat
    13321 cggcgtgctc gggttcggcc tcgtcggtgt cctcgccttc gtgaacatct tcctcgagcg
    13381 ccagtacggc atcggcgccg acggccgcgc cgtcatcggc atgatcaccg ccacggcctc
    13441 gttgctcggc acgctcgtcg ggggcaacgt cggcgagcga atcttcgccc gctccccggc
    13501 gcgcgccgtg cggatcgtgg gtgtggccat cgccggcttc tcgatgctgc tcgcgttcgc
    13561 cgtgttcctc ccgaccgtgt ggatgttcgt ccccgccgcc tgggtcggcc tgttcgcctt
    13621 gcaggtcggt atcgccccgc tctacaccgc gctctccgcc atcagcccgc cccggctgcg
    13681 ccccctgatg ttctcgctgc tcggcctcat ggtggccgtg atgggcggca tcgccggcgg
    13741 cgtcctcgtc ggcgccatcg ccgaggccac cgacatccgc tggggcctgg ccgccctcgc
    13801 cccgaccggg ctgatcggcg ggctgctcat ggcccgaggg gcgaccacca tcgaggccga
    13861 catcgcccgg gtcgccgagg agtccgtcgg tgacccgctg gcagcgttcc tgtagggaca
    13921 tcccggcgcc gggtcgccgg gtagggccag acatggcttc ttccaagtac cgagtgcacg
    13981 acgacggtgt ggccaaggcc cgccagatga tcgacgccaa ccagtacgac gtcgacacgg
    14041 agtggtccga tgccgcgccg tccgccgacg acggcaacga caagatcgac cgcgacggct
    14101 gggatggctt cggtgagtgg cacctcgcca tcgacaccga ggcgaacgag gagaccaagg
    14161 accgctacgg cttcccgtac ggcgacttcc gacgcgtctc ccgcagcggt ctcatccacg
    14221 ccaagcagcg agcgtcccag aacggccacg acgaggtcga gcgagcggcc ggcgagctgc
    14281 tggaccagct cgatcgggtc cgcaccgact gacgtcgccg gaccggatcg ttccgggcag
    14341 cggtgcacgc ctccggagag cgccgagcgt cccggttcct cagagggcgg cgaccacgtc
    14401 gaggaggcgg tccagctcgt cctcggtgtt gtagtagtgc gccgaggccc ggaccatggc
    14461 cggcagggcc cgcccgtcct ggaccgagtg gctcggcgtg gtggtcgagg tgttgaccgc
    14521 agctcgtgac agtgcggcct ggacgtcggc ggacggcacg ccatcgacgg tgaaggtgac
    14581 gatgccgccc ctgacgcgac ccttgtcgtg gacggagacc cggtcgagct cgcccagccg
    14641 gcggcggagc tgctcgccca ggtcccggag gcgctcccag ccggcggcca ccccgacctc
    14701 gagggcgtgg tcgacggccg cgccgaggcc gagccgggcc gcgtggctgc actcccaggt
    14761 ctcgaacctc cgggcatccc cgcggatctc gtagcggtcc gggccggtcc actcggcgga
    14821 gtgcaggtcg agcagcggcg gctcgatgcg ctcgatccac tcgtcgcgca cgtagagcaa
    14881 gccggtgccg cgtggccccc ggaggtactt gcgaccggtg gccgagagcg cgtcgcagcc
    14941 gagctcggtg acgtcgaggg ggagctggcc ggcggactgg caggcgtcga gcatgaagaa
    15001 cgccccgtgc tcccggcacc tggcgcccac ctcggccgcc gggttgacga gtccaccgct
    15061 ggtcggcacg tgggtcagcg acaccagcgc cgcaccccgg gccagctcgg catcgaggtg
    15121 gtcgaggtcg atctggccgt gctcgtcgtc gtcgaccagg acgatctcga gctcgtggcg
    15181 acgggccagc tgcagcagcg cgatggcgtt gctggcgtac tcggcccggg cggtgaggac
    15241 ccggtcgccg cgccggaacg ggaagccgta gacgaccatg tcccaggcgc gggtcgcgtt
    15301 gtccaccacc gcgatctccg gcggcgacgc gccgagcagc cgggcgatcg acgcgtacac
    15361 ggctgcggtc cgctccgccg cctcggccgc ggcctcgtag ccgccgatgg tcgcttcgcg
    15421 gcggaggtgc tcgaccacgg tgtcggtgac cacccggggc gggagggcgg cgccggcgtt
    15481 gttcaggtgg gcgacgtggt cgacgcccgg ggtgtcggcg cggacgcggg gcaggtcgac
    15541 gggcaccgca gcagtctggc cgccgtcacc gcccgcagag caggcagatc cggttcccgt
    15601 ggtcctcctc ggtgggctcg gacggttccg gctcccgttg cgggggtggg ggcgcggggt
    15661 ccgcacccgg cggggccggg tcggggtccg gcgccggctg gcgtggcgcg ggcggggcac
    15721 cggggatccg caggtcgaag tcgccgtcga cgtcgtccat cagcacggcg tcctgcgcgc
    15781 cgtagcgctg ctggctggtc gtctcggcag cgcggggccg ggcccacgcg aaggcctcct
    15841 cgaccgtgac ccgcccgtcg ccgtcgaggt cgccgaggcc ggcgccgaag gcctggtcga
    15901 tcaccagcga gccccacacc gagttgcccc aggacgggtg ctcgtaggac ttccgggcct
    15961 cgctcgagga gaacgtcagg atgcgtccgg gacgggccag gcccgggtcg gcgaagccgg
    16021 ccgcgttgca cgtggcgaag ctgagccaca tggcgtgcgg gcgcaccggg tcgagcagcc
    16081 ggaccagctc ggaatccacg atgaagcggt tgtcgctcgg ccacagcccc tcgtcggtga
    16141 cctcgccatc accgtcgacg tcctggccgt accacttctt ggagtggccc gagtagtgga
    16201 agacggcgac ggagtcctcg tcggtcttgt cggccagcca ggcgatgccc tcgacgatgc
    16261 cggcccgggt cgcctgccgg tcggtgagga gcaggatgtg gtcgtcccgc cacccccggg
    16321 cgaggagctg ggtgcggagg tcctcggcgt cctggcggga gccgaggttg tcggcgacgc
    16381 cgcggtgctc gttgatgccg atgagcagtg cccagcgggt ggtggccggg tccgcgggat
    16441 cctgggtggc ggcggcatgg gccgggaacc ggtcggcgaa ggtggcaccg gaggcgggcg
    16501 gtgccggcgg cggcggtgcg ggtgcggtcc gcgccgcgac ctgctcggtg accagctgcg
    16561 tcgcggccga cgggccggaa ggtgctgcgg ccgcagccat ccacgccggg gcgacgggcg
    16621 ggaccagcgg cgcggtcggc tccggtgccg cccgctcgtc cgccgacgcg gtgacgggcg
    16681 caggctgggc ccggtcctcg tcggctcgca cgatggccag cagctgcagc ggcgcgagca
    16741 gcgccagggc ggcgagcccg aagcgcgcat ggttcgtgcg tggcgtgagc aagcggtgac
    16801 ccccgggcgc tcgtgcggtg ccgagtcctg ggcgtcggca cgttccctgc acggggactt
    16861 cgaccagcgg tcgccgcgct cctgcctgcc ggggtcagga cgtgaggtcg tggtcgagca
    16921 ggtcgtcgac gtcgtcctcg ggcggtgcgg ccatggccca ccgtcccgtg gccttcgtga
    16981 acaggtacca gcgcccgtcc cggctgcgac ggagctgcag gcgcccctcg ccggtgaccc
    17041 ggttgccgga cacgtggacc cgccggatcg gcatcccggc ctcgaccatg agatcgcgcg
    17101 cccgggccat ctccagcggc ggggggttcc agcgcgcctc gtcgtgggcc cgcacgccgg
    17161 cggcgccgcc gaactgccac gccgcgccga gcacgtcgag gtcgaccgtg gcgacaccgc
    17221 tgtgctcggc caggcgctgc ttcacggccg gcggcgcgcc gatgacccgc cgggccaggt
    17281 cggcgcgctc gtccagctcc aggccggtgt cgaccgtgcc ggcgcacagc tcccaggccc
    17341 ggcgggcggc gtcggccacc agcgtgcgca gtccggcgcc gtcgaagggg gcatgcgccg
    17401 gcgggtcgct cggccacgcc gccgggcgac tcggagcgtg ggcgacgagg gggacgagcg
    17461 ggagggccag caggtcccgg ctccaggcct cggccgcgtc gatcccctcg ggggcggggg
    17521 gtgcggtctc gtcgtcctcg gcggtggcgg gcagaccgcc gcgccgccgc cggacctggt
    17581 cgaggacctc ctcccgtcgc cgcccgcgca gcagcagcag gacgaacggg tcggcgtcga
    17641 gggcgtcggc cagcaggtag caggccgcag cggcgtgctc gcacggctcg acgtcgtcgg
    17701 cgcaggtgca gacggtgtgc aggtcgcggg ggcggggcag caggtcgacc tcgaccgcct
    17761 cggcgtcgcg caccacggcg gggtcgagct cgccgtcgag cagggcggcg gcgtgggcgg
    17821 ccttggccgc gatggcgtcg agcaccgtgt cccactcgtc gtcgcggaac gcccggatgc
    17881 cgacgtgcac ctcgtggggc tcgtcccggt cggcctggac ccaggcgttc accccgcccg
    17941 gctcgatgtg caggtcggcg ccccggttgg ccttggccca gcggcgcccg cgagccaggc
    18001 gtccgccccg accgccggcg atgccctcga gcgcgtcgag ccaggcctcg ccccaccacg
    18061 tcgtgccgaa gtcgcgggcc atcagcggtc gaaccgcccg aggtcgaggc cgccgcccag
    18121 ctccaccaga tcggccagct cgtcgtcgga gagctccgac acccaggact cgccgccgcc
    18181 gacgacggac tcggccaggc cccgcttggc ctcgagcagc gtggcgatgc gctcctcgag
    18241 cgtgccctcg cagatcatgc ggtggacctg caccggccgg tcctggccga tgcgccaggc
    18301 ccggtcgctg gcctggtcct cgaccgccgg gttccaccag cggtcgtagt ggatgacgtg
    18361 ggtcgcccgg gtcaggttga ggccggtgcc gcccgcctgg agcgagatca cgaacaccat
    18421 cggcccgccg gcctggaacc ggtcgaccat ggcctcgcgc ccgggcaccg gcgtgccgcc
    18481 gtggaggaac tccacgtcga ggtcgagggc ggtgaggtgg cgggtgagca ggtgccccat
    18541 gcggacgtac tgggtgaaca cgagcaccga gtcgccctcg gacgcgatgg tctcgaccag
    18601 gtcggtgacc gcctcgagct tcccggaccg gccggtgatg gggctgtcct ggtcgaggta
    18661 ctgggccggg tggttgcaga tctgcttgag cgcggtcagg agcttgagga ccaggccccg
    18721 gcggttgatg ccctcggcct cggcaacgtc gtcgagcagc tcggtgacca ccgccttgta
    18781 gagggtggcc tgctcggtgg tgagcccgac gaagcggtcg gtctcggtct tgggcgggag
    18841 gtcgggggcg atctcgggat cggtcttgcg ccgccgcagc aggaacggcc ggaccagggc
    18901 ggccaggcgg gcggtggcct cctcgtcgcg ctcgcgctcg atgggggtgg ccacgtgctg
    18961 ctggaaggcg tcgagcttgc ccagcaggcc cggcgtggtg aagtcgagca gcgcccacag
    19021 gtcggccagg cggttctcga ccggcgtgcc ggtgagggcg aaccgggccg ccgccgggat
    19081 cgtgcggatc tgacgggccg tgcgcgacat ggggttcttc accgcctggg cctcgtcggc
    19141 cacgagcagg tcccacggct gctcggccag caccgcggcg tctcggcgca cgatgccgta
    19201 cgtggccacc acgagctcgt cgtcggcgag gcccgccagg ctccggtcgg cgccgtggta
    19261 gcggcgcacc ggcacgtccg ggcagaagcg ggccgcctcc cgctgccagt tgccgagcag
    19321 cgaggccggg cagacgatga gcgtgggtcc cgagccgcgg cgcagcaggt ggagggcgag
    19381 gatctggatg gtcttgccca ggcccatgtc gtcggcgagg acgccgccga ggccgagcga
    19441 cgccatctcc ttgagccagg ccaggccgcg ctcctggtag ggccgcaacg tggcctggag
    19501 ctcgggcggc gcggggtgcg cccgcatcgt gtcgatgctg cgcaaccggt cggcgaggtt
    19561 ccgcaggggc gcgtcgacct cggcctcgac cgtctcgccc ccgatggtga ccgtgcccga
    19621 cagcgcggcg gacagcacgt cgccggtgct gatcggggtg cgctcggcga ggcggcggag
    19681 gcgctcggga tcggcgcgga cccactggcc acgcagccgc acgacggggc gcttggcctc
    19741 ggccagctgc agcagctcgg cctcggtgag gtcaccgccg tcgatggtgg cgtgccagcg
    19801 catctcgccc agcgccgaga ggtcgagtcc ggccccgccc ccgcccgatt cgggagcggg
    19861 cgtgccgatg atgggtcgca gctcgaccgg gcgcagcacc tcggcgggcc acagcaccgg
    19921 gaggccggcg gcggccaggt cgtcggcgac cggaccgaac agctcctcgg cctcggcgtc
    19981 ctcgagcacc agcacctcgg gcgccgcctc ctgcagcagc cgctcgaccg gtgcccacac
    20041 ccgggccgcg gcgcgcaggg cgagcagcag gtcggtctcc acgtcggtgc cgaagcgggc
    20101 ggcgaccgcc gcgggcgcct gccacagctc ggcggcgtcg agcacgaggc tggggtccaa
    20161 gcggctctgc acctgcagca cgcagcgcac cggctcgtcg ggcccgtccg ggagcgcgat
    20221 gcgcaggccg ggggcggtgt cgctcgccgt gccggccacc gtggactgca gccaggactc
    20281 ggcgcccgtg acgtcggtgg cgtgggaggc ggcgaaggcc tcgaacccga cggtggcggc
    20341 cgccgcagcg gtgcgcacga aggagtcggc caccgcgtcg aggaacccgg cggtggccac
    20401 gttgggcgag gtcaggcgcg gcgacgtggc gtcgtccgcg accggcgtgg cgtgcgcggc
    20461 cggcggcagg gcgtcggcca gctggtccac gcgcgtgcgg tcggccacgt cgagggggcc
    20521 gaggcgccac atgtcggcac cgtcgggggc cacggcgggc aggaggcggc cccggctcac
    20581 ggcatcgagg gcgaggcggg tggcgaccga ccaggcccgc acggacgggc gcacctcggc
    20641 ggcggccggt agggcggcga gggtggcgac ggcatcgccg accgccacca ggtcggcggg
    20701 gagggtgcgg ggaccgtcgg cgagcaccac cgtgacctcg ccggtccggt cgatagcgtc
    20761 gtcgccgtcg acatcgaggt cccacagggc gagcgcgccg tcacggccgt ggaggccggc
    20821 cacgaacgtg gcttcggtcg tcggcgcgag acgcatccgg tcgacggtag cgacgggtgg
    20881 tgacagcgcg gccacactcg tggtcgtgga tcccatcctg acggtgcccc agggcacgtt
    20941 caccctcgaa cgagcccacc acgacccggg cgagccgctc cgggcgtggg acgccgccga
    21001 cgagctcgcc ctggcccacc tcgccgaggt cggggcggcc ggcgagcggt ggctcgtggt
    21061 cgacgacgcg cacggcgcgc tggccaccgc cctcgcgacc cacggcccga catcgtggac
    21121 ggactcgatc gtcacgatgg ccgcgacccg ggccaacctc gagcgcaacg gcctcgatcc
    21181 gtcggcagtg acctccgtgc ccagcacgtc ggaccccgcc ggcccggtgg acgtcgtcgt
    21241 ggtgaaggtg ccgcgcacgc tggcggtcct cgaggacgag ctccaccgcc tccgtccgct
    21301 cctgcacgcc ggctccgtcg tcgtcggcgc cggcatgacc aaggccgtgc accgctcgac
    21361 catcgccgcg ttcgagcagg ccatcggccc gacgcccacc tcacgggctc gcaagaaggc
    21421 ccgcctgctc ctggcctcgg tggacccgtc gctcgagccc gggccgggac ccggccccct
    21481 gcggtgggtg accccggacg gcctcgaggt caccggcctg cccggcgtgt tctcggccag
    21541 cagcctcgac ccgggcaccc gcctgctgct cgaccacctc cccgccccgg agccgggggc
    21601 cgtcgtggtc gacctcgggt gcggcaccgg cgtcgtgggc gccaccgtgg cccaccgcca
    21661 caccgacgtg tcgctcgtgt gctgcgacga gtcgttccgg gccgtggcat ccgcccgcca
    21721 gaccgtgggt gccgtggcct cggacgcgac cttccacgtc accgacgtcc tggacggggt
    21781 ggcggacgcg tcggccgacc tcgtgctctg caacccgccg ttccacaccg gtggcgcccg
    21841 caccacgtcc gtggcctacc ggatgttcgc cgactcggct cgcgtgctgc ggccaggcgg
    21901 caccatcacc gtcgtggcca accgccacct cggccaccac gtcgcgctgc ggcgctggtt
    21961 cgacgacgtc ggcgtggtcg gctccgaccc ccgcttcgtc gtcctgcggg gccgccggcg
    22021 ctgatcgtca ggcgtcggga gcggcgcgcc cggccagccc gtcgagcacg tcgtggaccc
    22081 agcgctcctg ctcgttgcgg cggcgacggc cgcgcaggcg ctgccgccac gtcgcggtcc
    22141 cgaacggctc gaggaccagg tcgaccttgg tccccgacgg gctcggccgg gtgcggaacc
    22201 agacccgctc ggcggcatcg atctgcacgc cgaaggtgtg ggtcggcccg cggacgatca
    22261 ccacgccctc gttgtggtcg gtgcggccgt tgcgctgccg gcggaacagg tagcggtcgt
    22321 cacccttggc gatgaccgga tcgagcagct cgggcatcca ggacggccac tgcgtgacct
    22381 cggtgacggc cgcgtagacg tcggccaccg gcgccgccac cacccgggtg gcgcgaggca
    22441 ggtcggtgcg ctcgaaggga gggcgctcgc tcaccccgca ccggtccggg gttcggtcac
    22501 gggctgctcg gtcggcgcag cgccctggtc ggccacgagg tcggtgagat cggcaaaccg
    22561 ggcgagcacg acggcctcga ggtcggcggc gcccacccgc tggaacgtct cgttcacgga
    22621 ggccatcacg gtgaggccga tgccggccag cagcagcggc cgcgggtcgg aaccggggtc
    22681 gacgccgagt cgctccgcca cggcgacggt gagcgcctgc tcccagcggg ggatcagcgt
    22741 gctgcgctgg tactcgccga ggctggtcgt ggatgcgctg atccgtgccc gccgcaggct
    22801 cagctcggcg gtcccggcga cctggcgggc caggacgagc accgcggccc ggatggcggc
    22861 cagcgggggc tcctccgggg gctgggacgt gatgacctcg tggagcagga tgagctggtc
    22921 gtcctggccg tcgtagagga cggcctcctt ggagccgaag taccggtaga aggtgcgagg
    22981 cgacaccacg gcggcgtcgg cgatctcctc gacggtcacc tcgtcgaagc cgcgctcctc
    23041 gaacagcgag agcgccacct gcacgaggcg ctgcctcgtg tgcgccttct tctgctcgcg
    23101 gagcccgccg tccatccgga cgacgctagg ggccgatctg tcaggtctgc cagttggcag
    23161 gcgtgacagt caatcgagca ggcggggcgg gaagccgccc cggctcaccg ggccccagtc
    23221 gtcgatggtg atgcgcagga ggaccttgcc ctggcgggtc atggcctcgc ggtactcgtc
    23281 ccagtccggg tgctccccgc tgatgctgcg gtagtagtcg cacagcggct cgagcgcgtc
    23341 gggcaggtcg agcacctcgc cggtgccgtg gacctgcacc cactcgtcgc cgaagtcgtc
    23401 ggacaggaac agcgcggtgg cccgggggtc ccgccgcagg ttggcggtct tggcccggtc
    23461 cgggtagctg gacaccacga cgtggccctg ggcgtcgatc ccgccggtga cggggctgag
    23521 ctgcacccgc ccgtcctggc gggtcgtgcc gaggatcagg tggtgccggg gccggacgaa
    23581 gtcgaggagc tcgtcccggc cgacggggag gtcgcgcgcc gcggtcggca cgctcagcgc
    23641 accccgagga ggtcgaccac gaacacgagc gtctcgttgg ggccgatgac cccgcccgca
    23701 ccctgcttgc cgtaggcctt gtccggcggc agcacgaggc gccggcggcc gccgaccttc
    23761 atgccctgca cgccctcgtc ccagccgctg atgaccatgc cggcgccgag gcggaacgag
    23821 aacggctcgc cccgatccca cgacgcatcg aactgctggc gggtcttcca ggacaccccg
    23881 acgtagtgga cgtcgaccgt gtcgccgctg gccgcggtgg gcccgtcgcc ctcctcgagg
    23941 tcctcgatca tcagcttgat gggtgggatc tcgcccggag ggacgtagac atggggcttc
    24001 tcggagtcgg ccatcccccg aggctaggac ctcggaccgg tgcgacgatc ccatactcct
    24061 gtcaccaatc gggacaacat ccagcgggga tccgcgcttt tcccctgatc accgccgaga
    24121 ctggatgtcg ggcccaacct ctggaggaca acacccatga acaagtcaga gctcatcgag
    24181 aagctggccg ccgacacgga cctgccgaag tcgcaggtct ccgccgtcgt ctcggccttc
    24241 gaggacacca tcgtcgccga ggtgaagtcg gggaacaagg tctccctgcc cggcttcctc
    24301 accttcgaaa aggggcaccg tgccgcgcgc acgggcaaga acccccagac gggcgaggcg
    24361 ctgcagatcg cagcggccga cgtgcccaag gtgaaggtcg gcaagagctt caaggacgcc
    24421 gtcaactcct gacgtcgccc cgaagcgaac gaacgtccgc tccggcgccg gcacctgccg
    24481 gcgccggtgg cgcgtgtaca cctgcgatgt ccgggcgtcg agcccggtcg atggttctgc
    24541 agagccgttg actgggtagt ggccttgcgt cggttgacga agttcgtcgt cccctgtgcc
    24601 gacgaacgaa aggtcatgca acgcatgcca agcatcacgt caccgccgcg atccacagct
    24661 ccgccaggac gaggtggacg ctggttccgc tcggccggtt tggccgccat cgctgcgctc
    24721 acggtcggca tcttcgccgt cagccccgcc ggggcggccg agccgcccgg cgcggtcacg
    24781 accagcgact tctgcgccaa cgcaggggac gcgtcgatgt tcgaggacat cgacgacagc
    24841 acgttcaccg acgagatcgt ctgcgccgag ctcctcggca tgacccaggg caccacgctg
    24901 acgacctacg accccgacgc cgacgtcacc cgcggtcaga tggccagctt catcgcccgg
    24961 atgatcgaca aggccagcga gctcgagctc ggcgaggttg ccgagctccc cgctgccgac
    25021 cccgacgcgg cctcgttcag cgacgtggac gacgagtccc cgcacgctga cgccatcgcc
    25081 cgcctggcgg cggtggacat cgtcctcggt ggccccggcg cccttcccgc caccgcctac
    25141 ggcccccagc tcccgatcga cagggaccag atggcatcgc tgatcaaccg ggccgtcggc
    25201 ttcatgaccg ggagcccgct ggccaccgat caggagtact tcaccgacgt ctccccggag
    25261 aacgtccacg ctgcgaacgt caacgcgctc gcggcggccg ggatcacgca aggcatcggc
    25321 aacgagatgt atgcgcccga ccaggcggtg acccgtggcc agatggccgc gttcctcgtc
    25381 cgctccttcg ccgtgctcgc cgaagcgggc gtcatcgcgc ccccgcctcc ggccgccaat
    25441 gccgccttcg cagccaccct cggggagctg aacggctccg gcgccgacgg caccgcgacc
    25501 gtcgtggtgg aaggcaccgt ggcgaccgtg agggtcgaca tcgccggcgc ctcgccggac
    25561 ctggtgcacg cacagcacat ccacatcggt ggcaacaacg agtgccccac gccggcgaac
    25621 gacaccgacg gtgacggctt catctccact ccggaaggcg ttccgtccta cggcggcatc
    25681 gcggtctcgc tgaccaccga gggcgacacc aacccggcca gcggcctggc catcgcccgg
    25741 atgcccgtcg catccgccag cggtgacgtc acctacgagc gcacgttccg gctgcccacc
    25801 ggcctgaccg ccgctgatgt ggcgaacggg gtcatcgtca gccacggcgt ggccgacacc
    25861 acgctcggca cggacgactc ggcctacgac ggccgcgaca gcccgttggc cgagggtgtg
    25921 ccccaggaag cgacgctccc cgcagcctgc ggtgcgctcg acgctcccgc cgaggccacc
    25981 tcgttccaga cggcgctcac ggagctgaac ggctcgaccg ccacggggac cgccacggtg
    26041 ctcgtgaacg gcaacctcgc caccgtgacc gtggacatcg ccggcgcctc gccgggcctg
    26101 gtgcacgcac agcacatcca catcggtggc aacaacgtgt gcccgacgcc cgccgacgac
    26161 accaacggtg acggcttcat ctccactccg gaaggcgttc cgtcctacgg cggcatcgcg
    26221 gtctcgctga ccaccgaggg cgacaccagc ccggccagcg gcctggccat cgcccgcatg
    26281 ccggttgcat cggacgccag cgtcgtgtcc tacgagcgct cgttcgtgct cccggccggc
    26341 gtcaccgccg ctgacgtgga ggacggcgtc atcgtgagcc acggcgtggc cgcgacgacg
    26401 ctgggcaccg accccgccac ctacgacggg ccagacagcc cgctggccga aggcgtgccc
    26461 caagaggcca cgctgccggc cgcgtgtggc gcgctggtcg cgacctgacg ccgaaggctc
    26521 ggccggtccc ggccgttcgg ctgaggtcgt tcgaccgaga cacatgacgc agcggagccc
    26581 cgaccggttc ggtcggggct ccgacgcgtg tcggccaggc agtcctgccc cccgctccgg
    26641 gctcggcgac gagacgcgca ggaaccgccg caaccatctg tcgagcctca gacgcggcgg
    26701 gcgtcctcga ccaggttgat ctcgtacagg acctggtcga gcaggtcggc ggcctcgatg
    26761 ccggcgtagc gggcgggcgt gtccggggtg accgtggagg gcagcggggc caggatcgtc
    26821 cacggcgtgg ggtggcagaa ctggacgacg gagtagcgct cgccgtcgtg gtcggggtcg
    26881 gccaccaccc ggtgccagcc cgacgggatg atcccgttcg acatccgctc gagcatgatc
    26941 ccggtgttga ggatcacgtg gccctccggc ggggcggcgt cgacccagac g
//
LOCUS       DATISU010000444         8233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_275502, whole genome shotgun sequence.
ACCESSION   DATISU010000444 DATISU010000000
VERSION     DATISU010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275502"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..858)
                     /gene="tesB"
                     /locus_tag="VLR27_17735"
     CDS             complement(4..858)
                     /gene="tesB"
                     /locus_tag="VLR27_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HSP05351.1"
                     /translation="MTQRALDAVVELLDLEPIEVNIFRGQSPDEDRQRTFGGQVAGQA
                     LVAASRTVESHALHSLHAYFLRGGDPNAPILYEVDRIRDGRSFTTRRVVAIQHGRAIF
                     HMSVSYQQPEDGFEHQFDSPIASTPPPDDLPDFQTRYAPLAEKLGDWVFRPRAIDTRY
                     VDWEPADRREKLPPRQRVWMRADGTLPDDPVLHQCVVTYASDMTLLDTTLLPHAKWHS
                     PDVFMASLDHAMWFHRPFRADEWLLYDQDTPTATGGRGLARGHLWDLDGNLVASVVQE
                     GLIRQMRR"
     gene            complement(1000..1506)
                     /locus_tag="VLR27_17740"
     CDS             complement(1000..1506)
                     /locus_tag="VLR27_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05352.1"
                     /translation="MPRIRTLTAVGVLAGATVLGGAGLAGAADTSEPKDQELIEDSQT
                     QQLTTFEGDEEIENEEAPTAPELPEAAADQARTALAEAPAFAGGDAESTDPDEETTEE
                     QQVVAPEDGTTDDSEADGTKPDNHGQRVSEVARSDDPGPGPDHGPAVSEEAKKNGQGG
                     DTDKTEED"
     gene            1625..2242
                     /locus_tag="VLR27_17745"
     CDS             1625..2242
                     /locus_tag="VLR27_17745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP05353.1"
                     /translation="MDPELRAAAEAARGFMPPDEGLALHDAAASVEPDGPFLEVGSYC
                     GKSGVYLGAAAAARGTVLFAVDHHRGSEENQPGWEFHEPDLVDPDVGRIDTLPRFRRT
                     IFDAGLEPHVVAVVGDSPTVARHWSTPLAFLFIDGGHGREPAHADFDRWVPHVCVGGT
                     LAIHDVFPDPADGGQPPYELYLRALAGGFDHVSTTGSLRVLRRTT"
     gene            complement(2308..3585)
                     /locus_tag="VLR27_17750"
     CDS             complement(2308..3585)
                     /locus_tag="VLR27_17750"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HSP05354.1"
                     /translation="MAPSTSRSTSTLRVALATCLAGAGVLVLPDPAAATTPDVLVVGD
                     SLTYDASDEIRDAMAAVGFRDVAVVAFGGTDIAWATEQLRARPEHPIVVLASGTNNTP
                     GGWSAGDRADAATAVQALRGRRCSTWVLPASNRHPHGRTIPDADAAATVEGIRSALSG
                     TGVHRTDWSAAAAGRPDWHQADGVHHTPAGQQAYAALLASDLRSACGPRPASTPVDAT
                     TSTDAAAYVDVVFRTFLGRPASAGERAEWAQRLATGTPRSTLTRALASSPEWIGVEID
                     TLYRKALGREADAGGREHWRRSVLSGRRIADIGAEMYGSLEFRQRAGGTDRAFVQALY
                     VEILGRDAEPAGLAHWESVLRRGVPHRDVAAGFYASLESRRQRVQALYERILQRPPDP
                     GGHAHWAAALQTRDDVRLAELLAASGELYDRSR"
     gene            complement(3668..4765)
                     /locus_tag="VLR27_17755"
     CDS             complement(3668..4765)
                     /locus_tag="VLR27_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05355.1"
                     /translation="MTTDASGGLPLPEIDGLLTAEESRQTAEAIAEWQLPSGMIPWFP
                     GGHADAWNHVEAAMALDIGGLRAESERAYEWLVGLQRPDGAWHQYYVSDDAGQISVEQ
                     DKLDANVCAYLACGVWHHFLRHKDAGFLEAMWPTVERAIDFVLDLQTPRGEILWARHA
                     DGSPWTFALLTGSSSICHSLRAAIGIAREVGHERSDWERSASALSHVVANVPDAFAPK
                     HRWAMDWYYPVLAGAVTGERGRTHLEDRYETFAKEDKGIRCVSDRPWITTAETCECAL
                     AYLSVGMRDRALELFTHAQTQRLDDGRYWTGIVYPQEVHFPADETSTYSAAAVLLAAE
                     ALHGATGGASLFVDHDDVLPVLPEHTPDDTL"
     gene            complement(4762..5532)
                     /locus_tag="VLR27_17760"
     CDS             complement(4762..5532)
                     /locus_tag="VLR27_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSP05356.1"
                     /translation="MLTVDYQRLGLQRGELVLDLGSGPGRHAFETLRRGARVVLCDYD
                     TGELTQARTLSSAMVETGEIASTDDIATTQGDGVFLPFPDDTFDRIIAAEVLEHIPDD
                     EGAIAELHRVLKPGGTIAVTIPAWFPEKVCWALSEEYHAPHVPGGHVRIYTENELRRK
                     LRDAGLKPGDAHHAHSLHSPYWWLRCAIDPQHSDDHPLVKAYLKLLTWDIVERPLVTR
                     VADKVLNPVLGKSLIVYARKPESDEVTGRGERSARAAA"
     gene            complement(5532..6839)
                     /locus_tag="VLR27_17765"
     CDS             complement(5532..6839)
                     /locus_tag="VLR27_17765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSP05357.1"
                     /translation="MRADGTTEDAGSEHGPLRIALLAYRGKPHVGGQGVYIRQLAKAL
                     TDLGHSVEILGGQPYPEVPAGVTLVELPSLDIDNEHVPARTPGFWEIKNWKDLVEVLS
                     FSAGQFPEPLAFSLRAWDHLKNRKDDFDIVHDNQTLGYGLLAIEAMGLPVLATIHHPI
                     TVDRRLELEHAPNWRKRWSLGRWYSFTHMQTQVARRLQRVVTVSENSFKDIVADHQVD
                     PDRMAIVPVGVDPDVFKPLPDVERIPGRLVTTASADVTMKGLKYLLEALAKLRTERDD
                     LHLVVIGKEKPGGPSSQTIDRLGLRGHVEFVTGVAERRIIELYSEAELAVVPSLYEGF
                     SLPAIEAMSCGCPLVATTGGAVPEVVGPDGETAMLVPPGDSEALAVRIKAALEDPEGS
                     RAIGLAGRERVKANWSWRHTAERTVAQYRMRLGQIPAQRGGVH"
     gene            complement(6915..7718)
                     /locus_tag="VLR27_17770"
     CDS             complement(6915..7718)
                     /locus_tag="VLR27_17770"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05358.1"
                     /translation="METLEAGGASVTIDHEHGGRLSSLVIGGNELLIDAMGRSDDPTS
                     WGCFPLAPFAGRIRHGRFSFEGRQVTLPRNHPPHAIHGTTHVQPWERTGPGTLRCDLG
                     PDWPWAGHAEQEVELTPTSLTLTLRVETTDDAMPATVGWHPWIRRNVGGADVEIVLPA
                     ERMWRRDDEDIPDGTLVTPTTGPWDDCFTALIGPVELHWPGVLDLYVESDCEHVVVFD
                     QPVGALCVEPQSAPPDAHNSETDLAVVTPDEPLVHRTTWAWSTPGGTPG"
     gene            7795..8232
                     /gene="dtd"
                     /locus_tag="VLR27_17775"
     CDS             7795..8232
                     /gene="dtd"
                     /locus_tag="VLR27_17775"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HSP05359.1"
                     /translation="MRALVQRVSEASVTVGGEVVGAIGPGLCVLIGVTHDDGPDHARK
                     LAAKLAKLRVLDDDAGVMNRSVLDEGGEVLVVSQFTLYGSTAKGNRPSWIDAARPEHA
                     EPLIDDVVAALREHGLTVATGRFQTEMSVALVNDGPVTVLLEI"
BASE COUNT         1060 a         2789 c         3173 g         1211 t
ORIGIN      
        1 cggtcagcga cgcatctggc ggatgagccc ctcctggacc acggaggcga ccaggttgcc
       61 gtcgaggtcc cagaggtgcc cgcgggccag gccccggccg ccggtggccg tcggcgtgtc
      121 ctggtcgtag agcagccact cgtcggcccg gaacgggcgg tggaaccaca tggcgtggtc
      181 caggctggcc atgaacacgt cggggctgtg ccacttggcg tgggggagca gggtggtgtc
      241 gagcagggtc atgtccgacg cgtacgtcac cacgcactgg tggagcaccg ggtcgtcggg
      301 gagggtgccg tcggcccgca tccagacgcg ctggcgcggg ggcagcttct ctcgtcggtc
      361 ggcgggttcc cagtccacgt agcgcgtgtc gatcgctcgt ggccggaaca cccagtcgcc
      421 gagcttctcc gccagcggcg cgtaccgggt ctggaagtcc gggaggtcgt ccggcggggg
      481 cgtgctggcg atcggtgagt cgaactggtg ctcgaagccg tcctcgggct gctggtagct
      541 gaccgacatg tggaagatcg cccggccgtg ctggatggcc acgacccgcc gggtggtgaa
      601 cgaccggccg tcgcggatgc ggtcgacctc gtagaggatc ggggcgttcg ggtcgccgcc
      661 ccggaggaag taggcgtgga gcgagtggag ggcgtgcgac tcgacggtgc ggctggccgc
      721 caccagcgcc tggcccgcca cctggccgcc gaaggtgcgc tggcggtcct catcggggga
      781 ctgccctcgg aagatgttca cctcgatggg ttcgaggtcg aggagctcga cgacggcatc
      841 gagggcacgc tgggtcaccg cgccatcctg ccccaccgcc cggacgcgac gaggcctcgg
      901 ccgaacccac gccgtccggg gtccggccga ggccgcgtca gccgtcagct cgtcggcacc
      961 gccaggaggc caccggcgcc gacatcgctg cgggtggggt cagtcctctt cggtcttgtc
     1021 ggtgtcgccg ccctggccgt tcttcttcgc ctcctcggag acggccgggc cgtggtccgg
     1081 gccggggccg gggtcatcgg agcgagcgac ctcggagacg cgctggccgt ggttgtccgg
     1141 cttggtgccg tcggcctcgg agtcgtcggt cgtgccgtcc tccggcgcga cgacctgctg
     1201 ctcctcggtg gtctcctcgt cggggtccgt cgactcggcg tcgccaccgg cgaaggcggg
     1261 ggcctcggcg agagcggtcc gtgcctggtc ggcagcggcc tcggggagct cgggcgccgt
     1321 cggcgcctcc tcgttctcga tctcctcgtc gccctcgaag gtcgtcagct gctgggtctg
     1381 cgaatcctcg atcagctcct ggtccttcgg ctcggaggtg tcggcggcgc cggcgagacc
     1441 ggctccgccg aggacggtgg cgccggcgag cacgccgaca gcggtgaggg tgcggatgcg
     1501 gggcatgtgg gtacctcgtc tggtttcggt cggggttgac attgcgaaca cattacgaat
     1561 ccatgacggc gctcgcaaca cttcttcgtc gacgccgtcg acgccacgta gcgtccgggc
     1621 cgtgatggat cccgagctcc gcgccgctgc cgaggccgcc cgcggcttca tgcccccgga
     1681 cgagggcctg gccctgcacg acgcggcggc atcggtcgag ccggacgggc ccttcctgga
     1741 ggtcggcagc tactgcggca agtccggcgt gtacctcggt gctgccgccg cggcccgggg
     1801 gaccgtgctc ttcgccgtcg accaccaccg gggctcggag gagaaccagc ccggctggga
     1861 gttccacgag cccgacctgg tcgaccccga cgtcggccgc atcgacacgc tgccgcgctt
     1921 ccgtcgcacg atcttcgacg ccggcctcga gccgcacgtg gtcgccgtcg tcggcgactc
     1981 accgacggtc gcccggcact ggtcgacccc gctggcgttc ctgttcatcg acggcggcca
     2041 cggccgcgaa ccggcccacg ccgacttcga ccgctgggtc ccccacgtct gcgtcggcgg
     2101 cacgctcgcc atccacgacg tcttccccga cccggccgac ggcggccagc cgccctacga
     2161 gctctacctg cgggccctcg ccggcggctt cgaccacgtc tccacgaccg gctccctccg
     2221 ggtcctgcgc cgcaccacct gaaccgatcc gttctggcct acatccatga ccaccaggtg
     2281 gcacatcggt aggccagaac gaattggtta gcggctgcgg tcgtagagct ccccggaagc
     2341 ggcgagcagc tcggcgaggc gcacgtcgtc acgcgtctgc agggcggccg cccagtgggc
     2401 gtgcccgccg gggtccggcg gccgctgcag gatccgctcg tagagcgcct gcacccgctg
     2461 gcggcgtgat tcgagcgatg cgtagaagcc ggcagcgacg tcgcggtggg ggacgccccg
     2521 tcgcagcacg gactcccagt gggcgaggcc ggcgggctcg gcatcgcggc cgaggatctc
     2581 gacgtagagg gcctgcacga aggctcggtc ggtgccgccg gcgcgctgtc ggaactccag
     2641 cgatccgtac atctcggcgc cgatgtcagc gattcgtcga ccactgagga cggagcgccg
     2701 ccagtgctcc cggccgccgg cgtcggcttc gcgcccgagg gcctttcggt agagcgtgtc
     2761 gatctccacg ccgatccact ccgggctgct ggcgagcgca cgggtcagcg tcgagcgcgg
     2821 cgtcccggtg gcgaggcgct gggcccactc ggcccgctca ccggcggacg caggtcgacc
     2881 gaggaacgtc cggaagacga cgtcgacgta ggccgcggcg tccgtcgacg tcgtcgcgtc
     2941 cacgggcgtg ctcgccggcc gtgggccgca ggccgaccgc agatcggagg cgagcagcgc
     3001 ggcgtaggcc tgctgccccg cgggcgtgtg gtgcaccccg tcggcctggt gccagtccgg
     3061 tcgcccggca gcggccgcgc tccagtcggt gcggtgcacg ccggtgccgc tgagcgcgct
     3121 gcggatgccc tcgacggtcg ccgcagcgtc ggcgtcgggg atggtgcggc cgtgcgggtg
     3181 gcggttcgat gccggcagga cccaggtcga gcagcgacgg ccgcgcaggg cctggaccgc
     3241 cgtggccgcg tcggcccggt cgccggcgct ccaaccgccc ggggtgttgt tggtgccgct
     3301 ggcgagcacg acgatcgggt gctcggggcg ggcgcggagc tgctccgtgg cccaggcgat
     3361 gtcggtgccc ccgaacgcga ccacggcgac gtcgcggaag ccgacggcag ccatggcgtc
     3421 gcggatctcg tcggaggcgt cgtacgtcag cgagtcaccc accacgagca cgtcgggcgt
     3481 cgtcgcagcg gccgggtccg ggaggacgag cacgccggca cccgccaggc acgtggccag
     3541 cgcgacgcgc agcgtcgagg tggagcggga cgtcgacggg gccatgcacc ctcatcgggc
     3601 gctcgaccag gtcgggacag atgcacccgc acccgacgaa atggtgcaga tgaaccaacc
     3661 gcggttctca gagggtgtcg tcgggggtgt gctcggggag cacgggcagc acgtcgtcgt
     3721 ggtcgacgaa gagggaggcc ccgccggtgg cgccgtggag cgcctcggcg gccagcagca
     3781 cggcggcggc cgagtaggtc gacgtctcgt cggccgggaa gtggacctcc tgggggtaca
     3841 cgatgccggt ccagtagcgg ccgtcgtcca ggcgctgggt ctgggcgtgg gtgaacagct
     3901 ccagggcccg gtcccgcatg ccgaccgaca ggtacgccag cgcgcactcg cacgtctcgg
     3961 cggtggtgat ccaggggcgg tccgacacgc agcggatgcc cttgtcctcc ttggcgaagg
     4021 tctcgtagcg gtcctcgagg tgggtgcggc cgcgctcccc ggtgacggcc ccggcgagga
     4081 cggggtagta ccagtccatg gcccagcggt gcttgggggc gaaggcgtcg ggcacgttcg
     4141 ccaccacgtg ggacagggcc gaggcggagc gctcccagtc cgaccgctcg tgaccgacct
     4201 cacgagcgat gccgatggcg gcccgcaacg agtggcagat cgaggacgaa ccggtgagca
     4261 gcgcgaaggt ccagggggaa ccgtcggcgt ggcgggccca gaggatctcg ccccggggcg
     4321 tctgcaggtc gagcacgaag tcgatggccc gctcgacggt gggccacatg gcctcgagga
     4381 agccggcgtc cttgtggcgc aggaagtggt gccagacgcc gcacgccagg taggcgcaca
     4441 cgttggcgtc gagcttgtcc tgctccaccg agatctgccc ggcgtcgtcg gacacgtagt
     4501 actggtgcca ggcgccgtcg gggcgctgca gcccgacgag ccactcgtag gcccgctcgg
     4561 actcggctcg cagcccgccg atgtcgagcg ccatggcggc ctcgacgtgg ttccacgcat
     4621 cggcgtggcc gccggggaac cacgggatca tgccggacgg cagctgccac tcggcgatgg
     4681 cctcggccgt ctggcgggac tcctcggcgg tgagcaggcc gtcgatctcc ggcaggggga
     4741 ggcctccgga ggcgtcggtg gtcacgcggc cgccctggcc gaccgctcac cccgcccggt
     4801 cacctcgtcg gactcgggct tgcgggcgta gacgatgagg ctcttgccca gcaccgggtt
     4861 gaggaccttg tcggcgaccc gcgtgacgag cggtcgctcg acgatgtccc acgtgaggag
     4921 cttcaggtac gccttgacga ggggatggtc gtcgctgtgc tgggggtcga tggcgcagcg
     4981 cagccaccag tacggcgagt gcagcgagtg ggcgtggtgg gcgtcgcccg gcttgaggcc
     5041 ggcgtcgcgc agcttccggc gcagctcgtt ctcggtgtag atccgcacgt ggccgcccgg
     5101 gacgtggggg gcgtggtact cctccgacag ggcccagcag accttctccg ggaaccaggc
     5161 cgggatggtc accgcgatgg tgccgccggg cttgagcacc cggtgcagct cggcgatggc
     5221 gccctcgtcg tcggggatgt gctcgagcac ctcggcggcg atgatgcggt cgaaggtgtc
     5281 gtcggggaac ggcaggaaca ccccgtcgcc ctgcgtggtg gcgatgtcgt cggtggaggc
     5341 gatctcgccg gtctccacca tcgccgacga cagcgtccgg gcctgggtca gctcgcccgt
     5401 gtcgtagtcg cacagcacga cgcgggcgcc gcgacgcagc gtctcgaagg cgtggcgacc
     5461 ggggccgctg ccgaggtcga ggacgagctc gccgcgctgc aggccgaggc gctggtagtc
     5521 gacggtgagc atcagtgcac gcccccgcgc tgggccggga tctggccgag gcgcatgcgg
     5581 tactgggcga cggtgcgctc ggcggtgtgg cgccagctcc agttggcctt gacgcgctcg
     5641 cggccggcga ggccgatggc gcgggagccc tcgggatcct cgagggcggc cttgatgcgc
     5701 acggccagcg cctcgctgtc gccgggcggc acgagcatgg cggtctcgcc gtcggggccg
     5761 acgacctcgg gcacggcacc gccggtggtg gcaaccagcg ggcacccgca cgacatggcc
     5821 tcgatggccg gcagcgagaa gccctcgtag agcgacggca cgacggccag ctcggcttcg
     5881 gagtacagct cgatgatgcg acgctcggcc acgccggtga cgaactcgac gtggccgcgc
     5941 aggccgaggc ggtcgatggt ctggctggac gggccgccgg gcttctcctt gccgatgacg
     6001 accaggtgca ggtcgtcgcg ctcggtgcgg agcttggcca gcgcctcgag caggtacttg
     6061 agccccttca tcgtcacgtc cgcgctcgcg gtggtgacca ggcggcccgg gatgcgctcg
     6121 acgtcgggca gcggcttgaa cacgtcgggg tccacgccca ccggcacgat ggccatgcgg
     6181 tcgggatcga cctggtggtc ggcgacgatg tccttgaagg agttctccga cacggtgacg
     6241 acccgctgga ggcggcgggc gacctgggtc tgcatgtgcg tgaacgagta ccagcggccg
     6301 aggctccagc gcttgcgcca gttgggtgcg tgttcgagct ccaggcggcg gtcgacggtg
     6361 atcgggtggt ggatcgtggc cagcaccggc aggcccatgg cctcgatggc gaggaggccg
     6421 tagcccaggg tctggttgtc gtggacgatg tcgaagtcgt ccttgcggtt cttcaggtgg
     6481 tcccaggccc ggagcgagaa cgcgaggggc tcggggaact ggccggcgga gaagctgagg
     6541 acctcgacca ggtccttcca gttcttgatc tcccagaagc cgggcgtgcg ggccgggacg
     6601 tgctcgttgt cgatgtccag gctgggcagc tcgacgagcg tgacgcccgc gggcacctcc
     6661 gggtacggct ggccgccgag gatctcgacg gagtggccga ggtcggtcag ggccttggcg
     6721 agctggcgga tgtagacgcc ctggccaccg acgtggggct tgccccggta cgccagcagg
     6781 gcgatgcgga ggggaccgtg ctcggagccg gcgtcctcgg tggttccgtc ggcgcgcata
     6841 gcggacaatc gtcgccggtc gttgactggt cggtcaagcg aggaccgggc gtctgcccgg
     6901 gctccgcacg ggtttcagcc cggcgttcct cccggcgtgg accacgccca cgtcgtgcgg
     6961 tgcacgaggg gttcgtcggg ggtgacgacg gccaggtcgg tctcggagtt gtgggcgtcc
     7021 ggcggtgcgg actgcggctc gacgcagagc gcgccgaccg gctggtcgaa gaccacgacg
     7081 tgctcgcagt cggattcgac gtagagatcg agcacgccgg gccagtgcag ctcgaccggt
     7141 ccgatgagcg cggtgaagca gtcgtcccac ggtccggtcg tgggggtgac gagcgtgccg
     7201 tcggggatgt cctcgtcgtc acgccgccac atgcgctccg cggggagcac gatctccacg
     7261 tcggcgccgc ccacgttgcg ccggatccag gggtgccacc cgacggtggc cggcatggcg
     7321 tcgtcggtgg tctcgacgcg cagcgtcagg gtcagcgagg tcggggtgag ctccacctcc
     7381 tgctcggcgt gcccggccca gggccagtcc gggccgaggt cgcagcgcag ggtgccgggc
     7441 ccggtgcgct cccacggctg cacgtgcgtg gtgccgtgga tggcgtgcgg ggggtggttg
     7501 cggggcagcg tcacctgccg gccctcgaag gagaagcggc cgtggcggat ccgcccggcg
     7561 aagggggcca gcgggaagca gccccaggac gtcgggtcgt cgctgcgtcc catggcgtcg
     7621 atgagcagct cgttgccgcc gatcacgagc gacgacagcc gaccgccgtg ctcgtggtcg
     7681 atggtgacgc tcgcgccacc ggcctccagg gtctccatcg gcccagtctc tcagggaccc
     7741 cgctcggaac tggcagtgcc cctgtcgcgg tgggctcccc taccctccgc cggcgtgcga
     7801 gcactggtgc agcgggtctc ggaggcgagc gtcaccgtcg gcggtgaggt ggtgggcgcc
     7861 atcggtccgg ggctgtgtgt gctcatcggg gtgacccacg acgacgggcc cgatcacgct
     7921 cgcaagctgg cggcgaagct ggccaagctg cgggtgctcg acgacgacgc cggcgtgatg
     7981 aaccggtcgg tgctcgacga gggcggcgag gtcctcgtcg tcagccagtt cacgctctac
     8041 ggcagcacgg ccaagggcaa ccggccgtcg tggatcgatg cggcccggcc cgagcacgcc
     8101 gagcccctga tcgacgacgt cgtggcggcg ctgcgcgagc acgggctgac ggtggccacc
     8161 gggcgcttcc agaccgagat gtcggtcgcc ctggtcaacg acggtccggt gacggtcctg
     8221 ctcgagatct gag
//
LOCUS       DATISU010000445         6983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_555237, whole genome shotgun sequence.
ACCESSION   DATISU010000445 DATISU010000000
VERSION     DATISU010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555237"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1088
                     /locus_tag="VLR27_17780"
     CDS             54..1088
                     /locus_tag="VLR27_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HSP05360.1"
                     /translation="MSKTNPGNFFEDFRVGQVIEHATPRTVTDGDRALYTALYPTRFA
                     LPSSDEFAAACGLSPAPVEELIGFHVAFGKTVPDISLNAVANLGYAECRFHQPVRPGD
                     TLRATSEVIGVKQNSNGKTGVVYVRSTATNQRGETAIDWARWVMVRKADVDAPAPETV
                     VPDLADAVAAEDLIVPAGLDFTGYDFAAAGEPHRFGDYEVGEKIDHVDGVTLEEAEHM
                     LATRLWQNTAKVHFNTEARDDGRRLIYGGHIISLARALQYNGMANAAMVAAINAGAHT
                     APAFAGDTVYAWSEVLDKAETSAPGVGALRVRLVATKGRDESMTLRGDDGKYADGVLL
                     DLDTWVLCPI"
     gene            1153..2241
                     /locus_tag="VLR27_17785"
     CDS             1153..2241
                     /locus_tag="VLR27_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05361.1"
                     /translation="MRLRSALLVAALATLIGVPVAHAVPDHARANGLRAVAVEDPPFS
                     IDAEQVRQDAVDCVPVEGAAPDVDAVVLVHGTSTRGHEQYAWNYELRLADEGIPYCVV
                     TYPERGFGDMQRSAEYVVVAVHEAAAISPSGDVDLVGHSQGATMPRWAVKYWPSVQVL
                     VDDFVLHAGPNHGTWAGDVATAPSLDGTPLAPLGARPPVFWQFRTDSDFNRYLNHGDE
                     TPGDIDYTSVYAHTDELVQPSGTNGPATAALEVDRPDKVVNVNVQDVCPARLVDHLSL
                     GTTDRFVMELTIATLRTPGPADLTSVDTTLCTLPDQYVTPATVPTLLGAGGATSFEEG
                     SAWLDPSRYVTEEPPVRDYAVAADGSAD"
     gene            2246..3109
                     /locus_tag="VLR27_17790"
     CDS             2246..3109
                     /locus_tag="VLR27_17790"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HSP05362.1"
                     /translation="MRPAQRDLIEWWSREGRDLPWRRSRDPWAVLVSELMLQQTQVSR
                     VTERYVRFLDRFPTVAACAAGPVGDVIDEWAGLGYNRRAVHLHGAAVHCVERHDGALP
                     DTLAELLALPGVGPYTARAVLVFAHERDIGLVDTNAGRFVARALAGRPLPPKEAQSVA
                     DDAVPTGWGWTWGQAVFDLGASVCRKRSPRCDGCPIRPHCAWATAGFPEPDPIAGSAG
                     ISAPQSRFDGSDRQGRGRLVDALRAGPVEHGALAAVMGWPDDPARAARVASTLERDGL
                     AIADHAGYRLP"
     gene            complement(3179..4234)
                     /locus_tag="VLR27_17795"
     CDS             complement(3179..4234)
                     /locus_tag="VLR27_17795"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HSP05363.1"
                     /translation="MPPEHRRGLRLIDTHYGGDVSRIVVGGVPRVPGDSVLAKRDWLQ
                     SEGDALRRLLLGSPYGDPSMCANVIVDTTLDRAEAGYVIMEAMGYPHFSGSNSICVTT
                     ALLESGHIPFGPPGHQTVHLEAPAGLIRAEVEHDGRHVHAVTLACDPAWVAERGRTAD
                     LPEFGTVTYDLVWSGCYYAVVDAEAQGFALTEAERPALAAFGLELCLVAGPELDLVHP
                     EFGDTGDLSFVNFAGPVRDGEDGPEAHTATYVHPGVVCRCPTGTGTAARLALLVDDGR
                     MDVGDRLTTISPTGSRMGGRVDDRITVSGQAATAVSITGQAFTLGITDVVVNLDDDLT
                     EAEGIWDLLAELDPDLP"
     gene            complement(4224..5339)
                     /locus_tag="VLR27_17800"
     CDS             complement(4224..5339)
                     /locus_tag="VLR27_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase family protein"
                     /protein_id="HSP05364.1"
                     /translation="MATERTLPETMHAVVLTGHGGLEMLEYRDDVPVPRPGPGEVLLE
                     ISACGMNNTDVWVREGAYGLEEDPGAVSTWRRGRSTLEFPRIQGTDSVGHIVEVGDGV
                     DGSRIGERVMVDFSIYNRDDDSLADIDYMGHGRDGGYAEYQVVPADHAHVVASDITDA
                     ELATFCCAYLTGEHMLDRVRLGSGERVLITGASGGVGSGLIQLCRARGAIPYAVTSAG
                     KADAVRAIGAEGVVLRDEGDLVEAVAEVTGGHDIDVVADLVAGDMFNDLLRILRPEGR
                     YTTSGAIGGPVVQLDLRTMYLKHLELHGSSQGTRGAFARLVGYIESGTIRPLLASTHR
                     LSEFHDAQRAFMAKGFIGNLVVVPDAKWDAVGAPHAA"
     gene            complement(5346..5864)
                     /locus_tag="VLR27_17805"
     CDS             complement(5346..5864)
                     /locus_tag="VLR27_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255296097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5586..5588),aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HSP05365.1"
                     /translation="MTTVLDPTGERQVAERALLERPASLEGKVVGLLDITKPRGDVFL
                     DHLEQGLRRRGVADVVRFAKPTYTKPAPVDLRHEISTKCDLVIEALADUGSCTSCSVH
                     DIADLERRGVPGVFIASNEFVDAAAAQAKALGFDAARVFVAHPIQDRTDDEMRDLADH
                     ALDEIVAAITGG"
     gene            complement(5877..>6983)
                     /locus_tag="VLR27_17810"
     CDS             complement(5877..>6983)
                     /locus_tag="VLR27_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HSP05366.1"
                     /translation="PGCGSLSVDPMRADELAGRFSASGLRSRRVEFADLEDDFEAMWD
                     RGWSDGLPLVPPTEARVARMLAGTTRAADEVVAVVPPDLVDCTVEKVAVNAVMAGCLP
                     EHLPVVLAALEAACTREFNMHGLLATTFFSGPVLIVNGPIRERIGMNSGVNALGQGNR
                     ANSTIGRALQLVVRNVGGGLPGGVDRATLGSPGKLGFCFAEDEEGSPFTPLSVDRGLD
                     PGVDAVTLFAGHGPTAIIDQKSREPESLARSFAAVLRTNAHPKLPLKIDAVLVVSPEH
                     GRIFREAGWGREQLMARLGELLLLDSDELIQGAGGIAEGIPEAFAGMQLPKFREGGLL
                     VCHAGGGAGLFSAVLGGWVNGEEGSDPVTVPIGT"
BASE COUNT          966 a         2551 c         2505 g          961 t
ORIGIN      
        1 ctgacggggg tcgatgggcg tccggcgccg gggcctttgt aggttctccc gccatgagca
       61 agacgaaccc gggcaacttc ttcgaggact tccgcgtcgg ccaggtcatc gagcacgcca
      121 ccccgcgcac cgtcaccgac ggggaccggg cgctctacac ggccctgtac ccgacccgct
      181 tcgccctgcc gtcctcggac gagttcgcgg ccgcctgcgg gctctcgccg gcgccggtcg
      241 aggagctcat cgggttccac gtcgcgttcg gcaagaccgt gccggacatc tcgctcaacg
      301 ccgtcgccaa cctgggatac gccgagtgcc gcttccacca gccggtgcgg ccgggcgaca
      361 ccctgcgagc cacctccgag gtcatcggcg tcaagcagaa ctccaacggc aagaccggcg
      421 tcgtctacgt ccgctccacc gccacgaacc agcggggcga gaccgccatc gactgggctc
      481 gctgggtgat ggtccgcaag gccgacgtcg acgccccggc gccggagacc gtcgtccccg
      541 acctggccga tgcggtcgcg gcggaggacc tgatcgtgcc cgccggcctg gacttcaccg
      601 gctacgactt cgccgccgcg ggggagccgc accgcttcgg cgactacgag gtcggcgaga
      661 agatcgacca cgtcgacggc gtcacgctcg aggaggccga gcacatgctc gccactcggc
      721 tgtggcagaa caccgccaaa gtccacttca acaccgaggc ccgcgacgac ggccgtcgcc
      781 tcatctacgg cggccacatc atcagcctgg cccgggcgct ccagtacaac ggcatggcca
      841 acgccgccat ggtggccgcc atcaacgccg gcgcccacac cgccccggcc ttcgccggcg
      901 acacggtgta cgcctggtcg gaggtcctcg acaaggccga gaccagcgcc cccggcgtgg
      961 gtgccctgcg cgtgcgcctc gtggcgacga agggccgaga cgagtccatg acgctgcggg
     1021 gcgacgacgg caagtacgcc gacggcgtgc tcctcgacct cgacacctgg gtgctctgcc
     1081 ccatctgaca ctgctggtga cgatgggcgc ccgggggcag ggctgcccgc ccgtcccgtc
     1141 gaacgacgag gtgtgcgcct ccgctccgcc ctcctcgtcg ccgccctcgc cacgctgatc
     1201 ggcgtcccgg tcgcgcacgc tgtcccggac cacgcccggg cgaacgggct gcgcgcggtg
     1261 gccgtcgagg atccgccctt cagcatcgac gccgagcagg tgcgacagga cgccgtcgac
     1321 tgcgtcccgg tcgagggcgc ggcgccggac gtggatgccg tcgtgctcgt ccacggcacc
     1381 tccacccggg gccacgagca gtacgcgtgg aactacgagc tgcgcctcgc cgacgagggc
     1441 atcccctact gcgtcgtcac ctacccggag cggggcttcg gcgacatgca gcgctccgcc
     1501 gagtacgtgg tggtcgcggt gcacgaggcg gcggcgatca gcccgagcgg cgacgtcgac
     1561 ctcgtcggcc acagccaggg ggcgaccatg ccccgctggg ccgtgaagta ctggccgtcg
     1621 gtgcaggtgc tggtcgacga cttcgtgctc cacgctggtc cgaaccacgg cacgtgggcc
     1681 ggcgacgtcg ccaccgcccc gtcgctcgac ggcaccccgc tcgccccgct gggcgcccgc
     1741 ccgccggtgt tctggcagtt ccgcaccgac tccgacttca accggtacct caaccacggc
     1801 gacgagaccc cgggcgacat cgactacacg agcgtctacg cccacaccga cgagctggtg
     1861 cagccgtcgg gcacgaacgg tccggcgacc gcggccctcg aggtcgaccg tcccgacaag
     1921 gtggtcaacg tcaacgtgca ggacgtgtgc ccggcccggc tggtcgacca cctctcgctc
     1981 ggcaccaccg accggttcgt gatggagctg acgatcgcca cgctgcggac gcccggaccc
     2041 gccgacctca cgtccgtgga cacgacgctg tgcacgctgc ccgaccagta cgtcacgccg
     2101 gcgacggtcc cgaccctgct cggtgcgggc ggggcgacgt cgttcgagga ggggtccgcc
     2161 tggctcgacc cgtctcgcta cgtcaccgag gagccgcccg tccgcgacta cgccgtcgcc
     2221 gccgacggga gcgcagactg accgggtgcg cccggcccag cgtgacctga tcgagtggtg
     2281 gtcccgcgag ggccgggacc tgccgtggcg tcgcagccgt gacccctggg cggtgctggt
     2341 gtccgagctg atgctccagc agacccaggt gagccgcgtc accgagcgct acgtccgctt
     2401 cctggaccgg ttcccgacgg tcgccgcctg cgcggcgggt ccggtcggcg acgtcatcga
     2461 cgagtgggcc ggcctcggct acaaccgccg agccgtccac ctccacggcg cggccgtcca
     2521 ctgcgtcgag cgccacgacg gtgcgctgcc cgacacgctg gccgagctgc tggcactgcc
     2581 cggcgtcggc ccctacaccg cccgcgccgt gctggtgttc gcccacgagc gcgacatcgg
     2641 cctggtcgac accaacgccg gtcggttcgt ggctcgggca ctggccgggc gaccgctccc
     2701 gccgaaggag gcccagtccg tcgctgacga cgccgtcccc accggttggg gctggacgtg
     2761 ggggcaggcg gtgttcgacc tcggcgcgtc ggtctgtcgc aagcggtcac cccggtgcga
     2821 cggctgcccg atccgccccc actgcgcctg ggcgacggcc ggcttcccgg agcccgatcc
     2881 gatcgccggc tccgccggca tcagcgcacc ccagtcccgc ttcgacggct cggaccgcca
     2941 gggtcgtggc cgcctggtgg atgccctgcg ggccggcccc gtcgagcacg gcgcgctcgc
     3001 cgcggtgatg ggctggcccg acgaccccgc ccgcgccgca cgcgtcgcct cgaccctgga
     3061 gcgggacggc ctcgccatcg ccgaccacgc cggctaccgc ctcccctgag cggtccgttc
     3121 tggcctacat ccgtgccgcc tgacggtcat ggatgtaggc cagaacgggt tcccggggtc
     3181 aggggaggtc ggggtcgagc tcggcgagca ggtcccagat cccctcggcc tcggtgaggt
     3241 cgtcgtcgag gttcacgacg acgtcggtga tgccgagcgt gaacgcctgg cccgtgatgc
     3301 tcaccgccgt cgccgcctgc ccgctgaccg tgatccgatc gtcgacccgg ccgcccatgc
     3361 ggctgccggt gggggagatg gtggtgaggc gatcgcccac gtccatccgg ccgtcgtcga
     3421 cgagcagcgc gagccgggcc gcggtgccgg tgccggtcgg gcagcggcac accaccccgg
     3481 gatggacgta ggtcgccgtg tgcgcctcgg ggccgtcttc gccgtcgcgg acgggaccgg
     3541 cgaagttcac gaacgagagg tcaccggtgt cgccgaactc ggggtgcacc aggtcgagct
     3601 ccggtccggc caccaggcac agctcgaggc cgaaggcggc cagggcgggt cgctccgcct
     3661 cggtcagcgc gaagccctgc gcctcggcat cgaccaccgc gtagtagcag cccgaccaga
     3721 ccaggtcgta ggtgacggtg ccgaactcgg gcagatcggc ggtgcggccc cgctcggcca
     3781 cccatgccgg gtcgcaggcc agcgtcacgg cgtgcacgtg gcgaccgtcg tgctcgacct
     3841 ccgcgcggat gaggccggcc ggcgcctcga ggtgcacggt ctggtgcccg ggcgggccga
     3901 aggggatgtg gccgctctcg agcagggcgg tggtgacgca gatggagttg gaacccgaga
     3961 agtgcgggta ccccatcgcc tccatgatca cgtagccggc ctcggcgcgg tccagggtgg
     4021 tgtcgacgat gacgttggcg cacatggacg ggtccccgta gggcgagccg agcagcaggc
     4081 gtcgcagggc gtcgccttcg gactggagcc agtcgcgctt ggcgagcacc gagtcgccgg
     4141 ggacgcgggg cacgccgccg acgacgatgc ggctgacgtc gccgccgtag tgggtgtcga
     4201 tcagccggag gccgcggcgg tgctcaggcg gcatggggtg ccccgaccgc atcccacttg
     4261 gcgtcgggga ccacgaccag gttcccgatg aagcccttgg ccatgaacgc tcgctgggcg
     4321 tcgtggaact cgctcagacg gtgtgtgctg gcgaggagtg gtcggatggt gccggactcg
     4381 atgtagccga cgaggcgggc gaacgcgccg cgcgtgccct gggacgagcc gtgcagctcg
     4441 aggtgcttca ggtacatggt ccgcaggtcg agctgcacca ccggaccacc gatggcaccc
     4501 gaggtcgtgt accggccctc cggtcgcagg atgcgcagca ggtcgttgaa catgtcgccg
     4561 gccaccaggt cggcgaccac gtcgatgtcg tgcccgccgg tgacctcggc gaccgcctcg
     4621 accagatcgc cctcgtcgcg caggacgacc ccttcggcgc cgatggctcg cacggcatcg
     4681 gccttgcccg cgctggtgac tgcgtagggg atggcgccgc gggctcggca cagctggatg
     4741 agccccgagc ccacaccccc ggacgcgccg gtgatgagga cccgctcgcc ggatccgagc
     4801 cgcacccggt cgagcatgtg ctcgccggtg aggtaggcgc agcagaacgt ggcgagctcg
     4861 gcgtcggtga tgtcgctggc caccacgtgg gcgtggtcgg cggggaccac ctggtactcg
     4921 gcgtagccgc cgtcacgacc gtgacccatg tagtcgatgt cggcgaggct gtcgtcgtcc
     4981 cggttgtaga tcgagaagtc gaccatgacc cgctcgccga tgcgggagcc gtcgaccccg
     5041 tcgccgacct cgacgatgtg gcccacgctg tcggtgccct ggatccgggg gaactcgagc
     5101 gtggagcgac cccgtcgcca ggtcgagacc gcacccgggt cctcctcgag gccgtacgcg
     5161 ccttcgcgca cccagacgtc ggtgttgttc atgccgcacg cgctgatctc gagcagcacc
     5221 tcgccggggc cgggccgggg gaccggcacg tcgtcgcggt actcgagcat ctcgaggccg
     5281 ccgtgtccgg tcagcaccac cgcgtgcatg gtctcgggca acgtgcgttc ggtcgccatg
     5341 tggcttcagc ctcccgtgat ggcggcgacg atctcgtcga gagcgtggtc ggcgagatcg
     5401 cgcatctcgt cgtcggtgcg gtcctggatg gggtgggcca cgaacacccg ggcggcgtcg
     5461 aacccgaggg ccttcgcctg cgcggccgct gcgtcgacga actcgttcga cgcgatgaac
     5521 acgccgggca cgccccggcg ctccagatca gcgatgtcgt gcacactgca cgacgtgcag
     5581 gaccctcagt cggcgagggc ctcgatcacc agatcgcact tggtggagat ctcgtggcgc
     5641 aggtcgaccg gagccggctt ggtgtaggtg ggcttggcga agcgcacgac gtcggcgacg
     5701 ccccgacggc gcaggccctg ctcgaggtga tcgaggaaga cgtcgccgcg gggcttggtg
     5761 atgtcgagca ggccgacgac cttgccctcg agggaggcag ggcgctcgag cagggcgcgc
     5821 tcggcgacct ggcgttcgcc ggtggggtcg aggaccgtgg tcatggccgc atcatctcat
     5881 gtcccgatcg gcaccgtcac cggatccgat ccctcctcgc cgttcaccca tccgccgagc
     5941 acggcggaga acaggccggc gcccccaccg gcgtggcaga ccagcaggcc gccctcccgg
     6001 aacttgggga gctgcatgcc ggcgaaggcc tcggggatcc cctcggcgat gccgccggcg
     6061 ccctggatca gctcgtcgga gtccaggagc aggagctctc cgagccgcgc catcagctgc
     6121 tcccgccccc acccggcctc ccggaagatc cgtccgtgct cggggctcac cacgagcacg
     6181 gcgtcgatct tgaggggcag cttggggtgg gcgttggtcc gcagcacggc ggcgaacgag
     6241 cgggcgaggg actcgggctc gcgcgacttc tggtcgatga tggcggtggg accgtggccg
     6301 gcgaagaggg tgacggcgtc gacgcccggg tccaggcctc ggtccacgga cagcggcgtg
     6361 aagggggagc cctcctcgtc ctcggcgaag cagaagccga gcttgccggg ggagccgagc
     6421 gtggcccggt cgacgccgcc gggcaggccg ccgcccacgt tgcgcacgac cagctgcagg
     6481 gcccgcccga tggtcgagtt cgcccggttg ccctggccga gcgcgttgac gcccgagttc
     6541 atgccgatgc gttcccggat cgggccgttg acgatcagga ccggacccga gaagaacgtg
     6601 gtggccagca ggccgtgcat gttgaactcc cgcgtgcacg cggcctcgag cgcggcgagc
     6661 acgaccggta ggtgctcggg caggcagccg gccatgaccg cgttcaccgc caccttctcg
     6721 accgtgcagt cgaccaggtc cggcgggacc acggcgacga cctcgtcggc cgcgcgggtc
     6781 gtgccggcca gcatgcgggc cacccgggcc tcggtgggcg ggacgagcgg cagcccgtcg
     6841 ctccagcccc ggtcccacat ggcctcgaag tcgtcctcga ggtcggcgaa ctcgacgcgc
     6901 cggctgcgca ggccgctggc cgagaaccga ccggccagct cgtcggcgcg catcggatcg
     6961 accgagagcg agccgcaacc cgg
//
LOCUS       DATISU010000446         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_741996, whole genome shotgun sequence.
ACCESSION   DATISU010000446 DATISU010000000
VERSION     DATISU010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741996"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2313)
                     /locus_tag="VLR27_17815"
     CDS             complement(<1..>2313)
                     /locus_tag="VLR27_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HSP05367.1"
                     /translation="LPAKQGLYDPAFEHDACGVAFVVDMHGRKSHAMVERGLDSLCNL
                     DHRGATGAEDNVGDGAGILTQIPDAFFRAVCDFDLPEAGSYAVGIGFLPQDAAADKAA
                     DAIDKIVHSEGLRLLGWREVPIDDSMIGRFAQEAQPTFRQLFLAGSEAEPLSGIELER
                     RAFVLRKRVEHEVEDVYFPSLSARTIVYKGMLTTPQLSEFFPDLRDERFISALALVHS
                     RFSTNTFPSWPLAHPYRVVAHNGEINTVQGNENWMRAREGAFASEAFGEHLDKALPIM
                     TAGASDTARFDECLELLHLAGRSLPHAVLMMVPEAWENHETMPDWKRAFYQYHASLME
                     PWDGPASIAFTDGTVIGAVLDRNGLRPSRYWVTEDGLVVMASEVGVLDIDPGTVVRKG
                     RLQPGRMFLVDTAEGRIVEDEEIKRGLAEAEPYEDWIHDGLIRLEDLPPRFLLTPQHS
                     SVVKHQRVFGYTSEELKILLAPMAAKGAEPIGSMGTDTPVAVLSERSRLLFDYFTQQF
                     AQVTNPPLDAIREEQVTSMQTTLGPEGNLLEPTPAACRQVVLPSPIISNEELAKLLYI
                     NDDGDMAGFKPFAIDGLYPVAEGGEGMRRAIHDVFHKVSDAIADGAKVIILSDRHSSS
                     ELAPIPSLLLASAVHHHLIREKTRTQVGLVLECGDAREVHHMALLIGYGVTAINPYLA
                     FETIDDLIDQGVIADISKPQAHRNFIKACTKGVLKIMSKMGISTVASYTGAQVFEAVG
                     LAQDLVDEFFTGTTSKLGGIGLDVIAEEVAR"
BASE COUNT          341 a          723 c          876 g          373 t
ORIGIN      
        1 cgggccacct cctcggcgat gacgtcgagg ccgatgccgc cgagcttgct ggtggtgccg
       61 gtgaagaact cgtccaccag gtcctgggcc aggccgacgg cctcgaacac ctgggcaccc
      121 gtgtaggacg ccacggtgga gatgcccatc ttggacatga tcttgagcac gcccttggtg
      181 caggccttga tgaagttgcg atgcgcctgg ggcttgctga tgtcggcgat gacgccctgg
      241 tcgatgaggt cgtcgatggt ctcgaaggcc aggtacgggt tgatggccgt gacgccgtac
      301 ccgatgagca gcgccatgtg gtgcacctcg cgggcgtcgc cgcactcgag gacgaggccg
      361 acctgggtgc gggtcttctc ccggatcagg tgatggtgca cggcggaggc cagcagcagc
      421 gacgggatcg gggccagctc gctgctcgag tggcggtccg agaggatgat caccttggcg
      481 ccgtcggcga tggcgtcgga caccttgtgg aacacgtcgt ggatcgcccg gcgcatgccc
      541 tcgccgccct cggcgacggg gtagaggccg tcgatggcga agggcttgaa cccggccatg
      601 tcgccgtcgt cgttgatgta gaggagcttg gccagctcct cgttggagat gatcgggctg
      661 ggcaggacga cctggcggca cgcggccggg gtcggctcga gcaggttccc ctcggggccg
      721 agggtggtct gcatcgacgt cacctgctcc tcgcggatgg cgtcgagtgg cgggttggtc
      781 acctgggcga actgctgggt gaagtagtcg aacagcaggc ggctgcgctc gctgagcacc
      841 gcgaccgggg tgtcggtgcc catcgacccg atcggctcgg cccccttggc cgccatcggt
      901 gccagcaaga tcttgagctc ctcgctggtg tagccgaaca cccgctggtg cttgaccacc
      961 gaggagtgct gcggggtcag gaggaaccgg ggcgggaggt cctcgaggcg gatgaggccg
     1021 tcgtggatcc agtcctcgta gggctcggcc tcggccaggc cccgcttgat ctcctcgtcc
     1081 tcgacgatgc gaccctcggc ggtgtccacg aggaacatgc ggcccggctg cagccggccc
     1141 ttgcgcacga cggtgccggg atcgatgtcg agcacgccga cctcggaggc catgacgacg
     1201 aggccgtcct cggtgaccca gtagcgagag gggcgcaggc cgttgcggtc cagcaccgca
     1261 ccgatgaccg tgccgtcggt gaaggcgatg gaggcggggc cgtcccaggg ctccatgagg
     1321 gaggcgtggt actggtagaa ggcccgcttc cagtcgggca tggtctcgtg gttctcccac
     1381 gcctcgggca ccatcatcag caccgcgtgc ggcagcgacc ggccggccag gtggagcagc
     1441 tccaggcact cgtcgaagcg ggcggtgtcc gatgcgcccg ccgtcatgat cggcagggcc
     1501 ttgtcgaggt gctcgccgaa ggcctcggag gcgaaggctc cttcgcgggc ccgcatccag
     1561 ttctcgttgc cctgcaccgt gttgatctcg ccgttgtggg cgacgacccg gtacgggtgg
     1621 gccagcggcc acgacgggaa cgtgttggtg gagaaccggg agtgcaccag cgcgagggcg
     1681 gaaatgaagc gctcgtcgcg caggtcgggg aagaactccg acagctgcgg cgtggtgagc
     1741 atgcccttgt agacgatggt ccgggccgag aggctcggga agtacacgtc ctccacctcg
     1801 tgctcgacgc gcttgcgaag cacgaaggcg cgccgctcga gctcgatgcc gctgagcggc
     1861 tcggcctcgc tgccggccag gaacagctgg cggaaggtgg gctgggcctc ctgggcgaag
     1921 cgcccgatca tcgagtcgtc gatgggcacc tcgcgccagc cgaggaggcg caggccctcg
     1981 ctgtgcacga tcttgtcgat ggcgtcggcg gccttgtcgg cggcggcgtc ctggggcagg
     2041 aacccgatgc ccacggcgta gctgccggcc tcggggaggt cgaagtcgca gacggcccgg
     2101 aagaaggcgt ccgggatctg ggtgaggatg ccggcgccgt caccgacgtt gtcctcggcg
     2161 ccggtggcgc cccggtggtc gaggttgcac agcgagtcca ggcctcgctc gaccatggcg
     2221 tgcgacttgc ggccgtgcat gtcgacgacg aaggcgacac cgcaggcgtc gtgctcgaag
     2281 gcggggtcgt agagaccctg cttcgcaggg aga
//
LOCUS       DATISU010000447        16867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_883407, whole genome shotgun sequence.
ACCESSION   DATISU010000447 DATISU010000000
VERSION     DATISU010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 16867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16867
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883407"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VLR27_17820"
     CDS             <1..445
                     /locus_tag="VLR27_17820"
                     /inference="COORDINATES: protein motif:HMM:NF040139.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05368.1"
                     /translation="IGTFFLTHPEIRIPEGSGSYAVNEANEPAWDDNVRDMVAAFRRQ
                     ESRCGARQARYSGALVADVHRILLKGGVFMYPPTTSKPNGKLRLLYENAPLAFLVAHA
                     GGSATDGRREILDITPTELHQRTPLYLGSSGDVAEVQRLLGSGPD"
     gene            663..1319
                     /locus_tag="VLR27_17825"
     CDS             663..1319
                     /locus_tag="VLR27_17825"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HSP05369.1"
                     /translation="MVPPLVRRAAPVAFLLLLASGIAACGSGDTDGPATLAPTTTLAG
                     VVRAPLLEVADVSLPEAPSGTPMAMRADDGELLVVYFGYTSCPDVCPMTMSDLRVAID
                     DLPEELADKVTVAMATVDPERDQPEVLSQYLGHFFDRTAALRTDDPDELAAAAKAFGV
                     QYEVEAHEPGDATYEVGHSAITYVVDDSGTVRVEWPFGFESVHMTTDLTALLTRPEIR
                     "
     gene            1316..1945
                     /locus_tag="VLR27_17830"
     CDS             1316..1945
                     /locus_tag="VLR27_17830"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HSP05370.1"
                     /translation="MTPTATATATRRLTAVAALVLAGSLLAACGDDDDVAAVSGDASP
                     VTEATDTTEAEEPAGELEVSDAWARTSPAVATAGAAYLDITNGTETDDVLVSASVDES
                     VAAKVELHETTAVEDDGDAGMGEGSGGDMGEGATDTSMAGAPMMQMQPVEDIPVPAGE
                     SVSLEPGGLHIMMLDLAEPLEVGTTIEITLTFEQAGEVVVDAEVRDMAP"
     gene            1942..2304
                     /locus_tag="VLR27_17835"
     CDS             1942..2304
                     /locus_tag="VLR27_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05371.1"
                     /translation="MTLRRRTLRLLLSGLVLLVAAGAIAGCGEDRQAQTVEVVVPAGT
                     QDRLDAGETVDVMPARIELRVGDTLLMRNEDDVDQTVGPYFVTAGGELSLTYSVAGRY
                     EGYCPLSGTDGYEIVVVD"
     gene            complement(2280..3611)
                     /locus_tag="VLR27_17840"
     CDS             complement(2280..3611)
                     /locus_tag="VLR27_17840"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSP05372.1"
                     /translation="MRRDPPDTSPDLASEVPERVVVPQSPVAPGTEPLERITTFHSLR
                     YPEYRLLFISGLFIFLGVQAQQIVRGWLAAELTGSNRGLGGVFFGFAIPMLLLTPFGG
                     VAADRFPKRTVLLVSTALLVISALWVGLADAFGVLEYWMLVGASAVQASGFAIFGPAR
                     ISFTSELVDRRALPNAVILSQISMNSTRIIGPAAGGALIGVAWLGTAGTYLITAALMV
                     IAFVIIFPLPRQAKVDRSDRPSPIAELRAGLRYVRGNRPVLLLIIASYAVVMFAFPYI
                     AFLPLVATEVFDVGSAGYGIMNAASGAAAVIVSLRIARFSSADSWRIQVVAGVVFGVG
                     IVALGLAPSFPLALLALFVIGGGASAFQAMNNTLILTESEPEYHGRVQSLLMLSFSGF
                     GLAALPLGAVADTVGLRPVFVGMGISAVVSMLLYQWARRRARRDQSTTTIS"
     gene            complement(3639..4469)
                     /locus_tag="VLR27_17845"
     CDS             complement(3639..4469)
                     /locus_tag="VLR27_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSP05373.1"
                     /translation="MPLSVTLLGTGSPLPDPNRAGPSTLVQAGDTNLLVDAGRGCVMR
                     LAGAGLFPSMVTGVLLTHLHSDHICALNDVITSHWVTSQAPTPLPIHGPPGTAAVVAG
                     IEQMLQPDVGYRLDHHDDLTAGPQLEVVEHGPGEAFTIGACTVTTHATDHRPVEPTLG
                     YRIEHGGAVAAIAGDTIPCAGLDELCAGADLYVQTVMRDDLVALVPNARLQDILDYHS
                     TVAQAAQTAARCGVATLVLTHYVPPLQPGQEDDWRSIAAEHFDGAIVLGDDLTRAGVD
                     "
     gene            4528..4959
                     /locus_tag="VLR27_17850"
     CDS             4528..4959
                     /locus_tag="VLR27_17850"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
                     /protein_id="HSP05374.1"
                     /translation="MDDESTPARKPSGVSWERWIERQIAEAIERGEFDGLAGAGLPIR
                     DLDARRDEMAWIRDKLRRERAAPPPPPAIQIRIDVDAALGSLGALTTEAEVRSLVDHL
                     NDRIRTVNRTTVSGPPTTLGPLPVEEVLERWHRLQGSSGGA"
     gene            complement(4974..5711)
                     /locus_tag="VLR27_17855"
     CDS             complement(4974..5711)
                     /locus_tag="VLR27_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05375.1"
                     /translation="MLVLSACGVDSGSDSAAPDAAAAETMELAELASARDTLPPAPHT
                     ATSLTVLLTHAGGADDPGIDAAAAALAQRPDIRVAIAATGTSPDGAVTMSGFPVTVVD
                     GTDLVASTIAATGDVPELVVVGIDRTDAPGDAEAIASAAAHAGVPALVVEVEGRDQVD
                     HAAAAMQLLEVLDLELDALLDEPAAVHRLIVPSCRSGSLRGRLVVRPAPVAASLGPSD
                     CTSTDRSVPDDEPSAVGAGYASLSRLP"
     gene            5814..6092
                     /locus_tag="VLR27_17860"
     CDS             5814..6092
                     /locus_tag="VLR27_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HSP05376.1"
                     /translation="MDIAAFQATIAATYGERDADRGTAPTVAWLCEEVGELAQAARKG
                     TPEQQLHELGDVIAWVASLAHQLGLSLDDAAQRYAAGCPVCASMPCDC"
     gene            complement(6100..6942)
                     /locus_tag="VLR27_17865"
     CDS             complement(6100..6942)
                     /locus_tag="VLR27_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05377.1"
                     /translation="MAVVFVCGALFALTLVGVARWRDEVFAAPAAPEAEDGPRARARH
                     YLWWLQVAVATALLCGLLVMGVGGRLAMRLLGATAGDAAQGRITEANEVVGEITVGGT
                     IGFVIFIGLLSGLVMTTLWFAVRRCLPERWIGGLLFGAGLLVVFGTRMEPLRPGNEDF
                     DLVGPWWLAVAAFSALALAFGVSLAAVSARLSRWLPLIGRDRRSLAYGSLLVLVVLFP
                     FGIAALVGGVAYVYGGRVVERVRSLLTGPTALRVAHLAIGAVVLVAAPGFVLAIGDLV
                     GRGP"
     gene            complement(6997..8022)
                     /locus_tag="VLR27_17870"
     CDS             complement(6997..8022)
                     /locus_tag="VLR27_17870"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HSP05378.1"
                     /translation="MRVGILGATGQVGGVMRQLLAERNFPVDELRYFASARSAGRTLP
                     WGDTEITVEDSTEADYSDLDVVLASAGATASRALAERIAASGPVVIDNSSAFRMDDRV
                     PLVVPEVNADALDDLPIGIVANPNCTTMVAMPVLKPLADEAGLEAIVVSTYQAVSGAG
                     LSGVEELDAQVRKVGNDAPGLTHDGRAAEFGEPDNFVKPIAFNVLPFAGDLVDDGLEE
                     TNEEQKLRNESRKILGLPELLVSGTCVRVPVFTGHSLSINARFSRPISPDRARELLAD
                     AAGVQLADVPTPLDSAGIDDTLVGRIRVDPTVEHGLALFLSGDNLRKGAALNTIQIAE
                     ELLRRRS"
     gene            complement(8073..9053)
                     /locus_tag="VLR27_17875"
     CDS             complement(8073..9053)
                     /locus_tag="VLR27_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05379.1"
                     /translation="MRTLLRTFAPLLLVLAVACGDDDDTTAASTTTAVPSTTTESTST
                     TTEAADPVDEDAAPYRVTVEDPGQEPRRELRLVVDAGDTDQVTLRQETSLEVDAGGQV
                     QSAPSPVNEFDVSYTIDEVDGDRFTATGVYDDVRVLDAPGVDPAVADELRSLMEGFRD
                     ARARTTFTSRGGIVEAELEGLELPGAAGAFAEQLAGSFTDSAESMSLPFPDEAIGQGA
                     RWRVDTETEIAGMPVEITTVVSLAELGEDRAAGTIEQTLRFVPGNVQVMGVAAEVISG
                     ELTGGGPIEWDLADGIVPRSDITIAGTAVMEANGVRIEQRQQQRITYLAR"
     gene            complement(9095..10306)
                     /locus_tag="VLR27_17880"
     CDS             complement(9095..10306)
                     /locus_tag="VLR27_17880"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HSP05380.1"
                     /translation="MITVQKFGGTSVADPERIREVAEHAVRTKRRGTDVVLVVSAMGK
                     ETDALLRLADDVSTTQPGREMDMLITAGERKAMALVAMAISDLGVPSQSFTGSQAGFI
                     TDTNHKDAKILELRPERIADALAEGFIPVIGGSQGVSIERDVTFLGRGGSDTTAVAIA
                     HALGAEFCELYTDVAGVYSADPRIVSKARRMAEISFDELLEMTATGCPKPAMRSVEFA
                     RRHHVKLHIRSAFTWEPGTIVTEETTDMEQAVVNAVTHDTSEAKVTVSGVSDQPGVAA
                     QMFTSLAGAGVNVDMIVQNVSDHGRTDISFTCPHSDAERATELINSLSGHVGNAGVGF
                     DHDIGRVSVVGAGMKSNPGVAAKMFQVLADNDINIEMISTSAIRISCVIQQSQVDDAV
                     QVLHTAFDLDA"
     gene            complement(10347..10832)
                     /locus_tag="VLR27_17885"
     CDS             complement(10347..10832)
                     /locus_tag="VLR27_17885"
                     /inference="COORDINATES: protein motif:HMM:NF045311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HSP05381.1"
                     /translation="MASDDDSLSSFSVEVGQRLRAVRRLRKLSLDDVERESGGQWSAS
                     AIGAYERGFRNLSLPRLRELAEFYDVPMSVLLGENDDDDDSDRPSKVILDLVALSGIP
                     EAEAVHRYARSIILERGDFNGRVLSIRRDDLRAICSLLHSTETQTLQQLRDWDALVET
                     L"
     gene            11033..11986
                     /locus_tag="VLR27_17890"
     CDS             11033..11986
                     /locus_tag="VLR27_17890"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSP05382.1"
                     /translation="MGTSKRERQKMARHEKIEELRRQEQRQATRKRGVLIGLGIAAFL
                     AFAIGYSVLTSDDDGDDEVATADDTEAEGAEDETATEDETATEDDGTTDTTEGTSETT
                     ETTVAEVQPGDTECPPADGSAERQIDFDGPFQDCLEDGVDYQARFTTTEGEVLVDLLE
                     EQMPNTVNNFVALARYGYYDDTLLFRTDPSIDIIQGGSPHTQDNTDQGPGYNIPDEGG
                     EFTSDAATGQYTGPFTYEEGQLIMARSMGPDSSSAQFFFSTGPNVELLDSQGTYLLFG
                     DVVEGLDVLQEIIGLHEDDDSGLGGAPSRDVTIESVEILEL"
     gene            complement(11952..13595)
                     /locus_tag="VLR27_17895"
     CDS             complement(11952..13595)
                     /locus_tag="VLR27_17895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP05383.1"
                     /translation="MANRFPDPHQRGALLQGAAAVLAVVAVVVALVQGAMLTALVATV
                     VAALVGLEVRRRGQVTLTAPELDRLREAELALSRATDTHEAARELAEHAIALLGARSA
                     VVLIEGIGDTVRVAAGPTATEDVYGSGSRMRLLDDAGLPCGSIAVSARADGRPYAARH
                     ERILDALAERVSTTLHQLSLLTDVSTERRTLADLVGSSSDGIFSVGSDLRVRAWNPAM
                     ETITGVPAEVAVGEHVSQSFRPTTESGEPATGSADPGRVGRPVTGVVLRIEAGDEERW
                     LTCSYAPLSDGGYVAVARDETARKKLQDDKDGWIAQVSHELRTPLTPIKGFLHTLARR
                     DAELTTEDRARIYEIMIREEQRLEALVDSLLRSTQLDAGGMVVERTEVDWADRVLEQV
                     ELVRRQDPSRTIDLDVDPDAGPVLADEGLAIGILTNLLSNALKYAEGDGGVEVLVEAD
                     GDELITSVIDHGPGIPRSDRTRVFDKFTRLGNHLTRSQQGVGLGLHIAKQSIERLDGR
                     IWVDETPGGGATFRFTLPRTERSRRRAPLRAPGSPRTRS"
     gene            complement(13704..14243)
                     /locus_tag="VLR27_17900"
     CDS             complement(13704..14243)
                     /locus_tag="VLR27_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05384.1"
                     /translation="MRARRRGLTRHGRGRLLDLGGADMHRNLWTGADGVSDTTVLDGV
                     GDRRLGELAEAGERFDTVVSVFQLAASTDLTETLRSVRRVLADDGRVLFLEPGAQVGL
                     PGRLQRLVAPPLGGLTGWRADRDIPAELRTAGLSVIDIRRHRVPTLQLWLRQVLEGVA
                     HHALPPGDGTPPAPPAQPA"
     gene            14364..15212
                     /locus_tag="VLR27_17905"
     CDS             14364..15212
                     /locus_tag="VLR27_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HSP05385.1"
                     /translation="MTIKSVGIIGSGIMGSGIAQVAATSGFDVVLRSRKQESAEQTVA
                     AMQKSLEKLVAKERMSQEDADAAVARVTATSKLDDLADCDLVIESVVEDLATKVALFN
                     DLDHHCKDDAILATNTSTLSVIEMAVETRRPERVCGVHFFNPAPMMSLVEIIKTLVTS
                     DETIAEVRQFAEACGKSPVEVKDRAGFIVNHLLFPYLNNAVRMLENGTASRDDIDSAM
                     KGGANFPMGPFALLDLVGLDTSLSILDALYDEFRDPHYAASPMLRRMVSAGHLGRKSG
                     AGFYEY"
     gene            15437..16108
                     /gene="aat"
                     /locus_tag="VLR27_17910"
     CDS             15437..16108
                     /gene="aat"
                     /locus_tag="VLR27_17910"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HSP05386.1"
                     /translation="MPVEPPPCAWSFPDVDTADESGVVGVGGDLSPGTLLHAYRSGLF
                     PMQVDRGRTLAWWSPDPRGVLPLDGLRVSRSLRRSCARFEIRVDTCFDEVVASCADPK
                     RPHGWITEEIRQAYRQMHRLGWAHSVEAWSREDGTLAGGLYGVSVGGLFAGESMFHRE
                     RDASKVALVGLVDILSADGVNRLLDVQWTTDHLRSLGAIDVPRNDYIDRLSVALRLPM
                     PPAFI"
     gene            complement(16137..16820)
                     /locus_tag="VLR27_17915"
     CDS             complement(16137..16820)
                     /locus_tag="VLR27_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05387.1"
                     /translation="MIRSLAALFAKKTAIVAITGVTVIGGATLALAVEGDPPTDDPAA
                     ALLEPCEGGVDRDEDGSCPEDEPKPIEPCDDGTTPAEGEACPEDEPKPAEPCDDGTTP
                     AEGEACPEDDEGAENDELVDDETDDDVVAEHPENHGKYVSEAAKGGGECAGLSGRDKG
                     QCVAGVAKSDIGKKTAEEPAAETTEKTVTEQSAPVQEPAAAAVTEEQPKGKPAHAGPP
                     AGKGKGGKG"
BASE COUNT         2425 a         5865 c         6248 g         2329 t
ORIGIN      
        1 gatcggcacc ttcttcctca cccatccgga gatccgcatc ccggagggct cgggcagcta
       61 cgcggtcaac gaggccaacg agcctgcgtg ggacgacaac gtccgcgaca tggtcgccgc
      121 cttccgtcgg caggagtccc gctgcggagc ccgccaggcc cgatactccg gcgcgctggt
      181 cgccgacgtc caccgcatcc tgctgaaggg cggcgtcttc atgtacccgc cgacgacgtc
      241 gaagccgaac ggcaagctgc ggctgctcta cgagaacgcg ccgctcgcgt tcctcgtcgc
      301 ccacgccggc ggctcggcga cggacggacg tcgcgagatc ctcgacatca cgccgacgga
      361 gctgcaccag cggacacccc tgtacctcgg cagcagcggc gacgtcgccg aggtgcagcg
      421 actgctcggc tccggaccgg actgaccctc ctcgcccggg gcagggcgtc gaccgatgag
      481 tctgcgccct ccgtccccgc aggcatgccc atgggcaaga tcgacatcgg cgcgctcgag
      541 gcggcccacg ccggggagca gtagcgaccg tcgggctgca agtggcgtga attgcgtcac
      601 caccccatca gcagtgacct gaggcccttc ctgcgttctg ggcgtcgccg tacgttccga
      661 tcatggtccc cccactggtg cgccgggctg cgccggtcgc cttcctcctg ctgctcgcct
      721 cggggatcgc cgcctgcggc tcgggtgaca cggacgggcc ggccacgctc gcgcccacca
      781 cgaccctcgc cggcgtggtg cgggcgccgt tgctcgaggt ggccgacgtg tcgttgcccg
      841 aggcgcccag cgggacgccg atggccatgc gcgccgacga tggcgagctg ctggtcgtct
      901 acttcgggta cacgtcctgc cccgacgtct gcccgatgac gatgagcgac ctgcgcgtcg
      961 ccatcgatga cctccccgag gagctcgcgg acaaggtgac cgtggcgatg gccaccgtcg
     1021 acccggagcg cgaccagccc gaggtcctct cccagtacct cgggcacttc ttcgaccgga
     1081 cggccgcgct gcggaccgac gaccccgacg agctggccgc cgccgccaag gcgttcggcg
     1141 tgcagtacga ggtcgaggcc cacgagccgg gcgacgccac ctacgaggtc gggcactcgg
     1201 cgatcaccta cgtggtcgac gactccggca ccgtgcgcgt cgagtggccc ttcgggttcg
     1261 agagcgtgca catgacgacc gacctgaccg ccctgttgac ccgaccggag atccgatgac
     1321 ccccaccgcc accgccaccg ccacccgccg cctgaccgcc gtcgccgccc tcgtgctcgc
     1381 cggctccctc ctcgccgcgt gcggtgacga cgacgacgtc gccgccgtgt ccggcgacgc
     1441 gtccccggtc accgaggcca ccgacacgac ggaggccgag gagccggctg gtgagctcga
     1501 ggtctcggac gcctgggccc gcacctcgcc ggccgtggcc accgcgggcg cggcctacct
     1561 cgacatcacg aacgggaccg agaccgacga cgtgctggtc tcggcgagcg tcgacgagtc
     1621 cgtggcggcg aaggtcgagc tccacgagac gaccgccgtc gaggacgacg gcgacgccgg
     1681 gatgggcgag gggtcgggtg gcgacatggg cgagggggcc accgacacct cgatggcggg
     1741 cgccccgatg atgcagatgc agccggtgga ggacatcccg gtgccggccg gcgagagcgt
     1801 gtcgctcgaa cccggcgggc tgcacatcat gatgctggac ctcgccgagc ccctcgaggt
     1861 gggcacgacg atcgagatca cgctcacctt cgagcaggcc ggtgaggtcg tggtcgacgc
     1921 cgaggtccgg gacatggccc cgtgaccctg cgccggcgga cactgcggct gctgctgagc
     1981 ggcctggtcc tcctggtcgc cgccggcgcg atcgccggct gcggcgagga tcgccaggcc
     2041 cagacggtgg aggtggtcgt cccggccggc acccaggacc gcctcgacgc gggcgagacc
     2101 gtcgacgtca tgccggcccg catcgagctg cgggtgggcg acacgctcct gatgcgcaac
     2161 gaggacgacg tcgaccagac cgtcggtccc tacttcgtga ccgccggcgg ggagctctcg
     2221 ctcacctaca gcgtggccgg ccggtacgag ggctactgcc cgctgtcggg gaccgacggc
     2281 tacgagatcg tcgtcgtgga ctgatcgcgc cgcgcccggc ggcgggccca ctggtagagc
     2341 agcatcgaga cgaccgcgct gatgcccatg ccgacgaaca ccggccgcag cccgacggtg
     2401 tcggcgacgg cacccagggg gagcgcagcg agcccgaacc cggagaagct cagcatgagc
     2461 agcgactgca cccgcccgtg gtactcgggc tcgctctccg tgaggatcag ggtgttgttc
     2521 atcgcctgga aggccgaggc gccgccgccg atgacgaaga gggcgagcag ggcgagcggg
     2581 aacgacggcg cgagcccgag ggcgacgatg ccgacgccga agaccacgcc ggcgacgacc
     2641 tggatgcgcc agctgtcggc gctggagaac cgggcgatgc ggagcgagac gatcaccgcg
     2701 gcggcgccgc tggcggcgtt catgatcccg tagccggccg agcccacgtc gaagacctcg
     2761 gtggccacga gcgggaggaa ggcgatgtag gggaaggcga acatcaccac tgcgtacgag
     2821 gcgatgatca gcaggaggac cgggcggttg ccgcgcacgt agcgcagccc ggcgcgcagc
     2881 tcggcgatcg ggctcggccg gtcgctgcga tcgaccttgg cctgtcgggg cagcgggaag
     2941 atgatcacga aggcgatgac catcagcgct gcggtgatga ggtaggtgcc ggcggtgccg
     3001 agccaggcga ccccgatgag ggcgccgccg gccgcggggc cgatgatccg cgtgctgttc
     3061 atgctgatct ggctgaggat cacggcgttg ggcagcgccc ggcggtccac cagctcgctg
     3121 gtgaagctga tccgggccgg gccgaagatg gcgaaacctg atgcctggac ggcggacgcg
     3181 ccgacgagca tccagtactc gagcaccccg aaggcgtcgg cgaggccgac ccacagtgcg
     3241 ctgatcacca gcagggccgt cgagaccagc aggaccgtgc gcttggggaa gcggtcagcg
     3301 gcgacaccac cgaagggtgt gaggagcagc atcgggatgg cgaagccgaa gaacacgccg
     3361 ccgaggcccc ggttggagcc ggtgagctcc gcggcgagcc aaccccgcac gatctgctgg
     3421 gcctgcacgc cgaggaagat gaacaggccg gagatgaaca gcagccggta ctcggggtac
     3481 cgcagcgagt ggaacgtggt gatccgctcg agcggctccg tgcccggggc gacgggggac
     3541 tgcgggacga cgacgcgctc ggggacctca gatgccaggt cgggagaggt gtccgggggg
     3601 tctcggcgca tcgccggccc accttattga ccgcgcggtc aatccacgcc ggcccgggtg
     3661 aggtcgtcgc cgaggacgat cgccccgtcg aagtgctcgg cggcgatgct ccgccagtcg
     3721 tcctcctggc cgggctgcag cggcggtacg tagtgggtga ggacgagcgt ggcgacaccg
     3781 caccgggccg cggtctgcgc tgcctgcgcc acggtcgagt ggtagtcgag gatgtcctgc
     3841 agccgggcgt tgggcacgag ggccacgagg tcgtcgcgca tcacggtctg gacgtagagg
     3901 tcggccccgg cgcacagctc gtcgaggccg gcgcacggga tcgtgtcgcc ggcgatggcg
     3961 gcgacggctc ccccgtgctc gatccggtag ccgagggtgg gctcgacggg ccggtgatcg
     4021 gtggcgtggg tcgtcaccgt gcaggcgccg atcgtgaacg cctcgccggg cccgtgctcc
     4081 acgacctcga gctggggccc ggcggtgagg tcgtcgtggt ggtcgagccg gtagcccacg
     4141 tcgggctgca gcatctgctc gatgccggcg acgacggcgg cggtgcccgg cggaccgtgg
     4201 atcggcagcg gggtcggcgc ctggctggtc acccagtgcg acgtgatcac gtcgttcagg
     4261 gcgcagatgt ggtcgctgtg caggtgggtg agcagcacgc cggtgaccat gctcgggaac
     4321 agcccggcgc ccgccagtcg catgacgcac ccacgtccgg catcgacgag gaggttggtg
     4381 tcgccggctt gcacgagggt cgacggtccg gcccggttcg ggtcggggag cgggctgccc
     4441 gtcccgagca gcgtcacgga gaggggcatg gggacagccg aacatctgcg agccagactg
     4501 tcaaatgatt cccgtatcgt cgccggcgtg gacgacgagt cgacgccggc acgcaagcca
     4561 tccggggtct cgtgggagcg ctggatcgag cgacagatcg ccgaggcgat cgagcgcggc
     4621 gagttcgacg gcctcgcggg cgccggcctc ccgatccgcg acctggatgc tcgccgggac
     4681 gagatggcct ggatccggga caagctccgc cgcgagcgcg ccgcgccgcc tccgcctccc
     4741 gcgatccaga tccgcatcga cgtcgatgcc gccctcggtt cgctcggggc gctgacgacc
     4801 gaggcggagg tgcggtcgct ggtcgaccac ctcaacgacc ggatccgcac ggtgaaccgc
     4861 accacggtct cgggaccacc gaccacgctc ggcccgctcc cggtggaaga ggtgctcgag
     4921 cgctggcacc gactgcaggg ctcgagcgga ggcgcctgaa cccggcgtgg ggatcagggc
     4981 agtcggctca gggaggcgta gccggcaccc accgccgacg gctcgtcatc gggaacggac
     5041 cgatcggtgc tggtgcagtc cgaagggccg agcgacgccg cgacaggggc aggccgcacc
     5101 acgagccggc cccgaagcga gccggaccga cagctcggca cgatgagccg gtggacggcc
     5161 gccggctcgt cgaggagggc atccagctcc aggtcgagca cctcgagcag ctgcatcgcc
     5221 gcggcagcgt gatcgacctg gtcgcggccc tccacctcca ccacgagtgc cgggacgccg
     5281 gcgtgggccg cggcggaggc gatcgcctcg gcgtcgccgg gggcgtccgt gcgatcgatg
     5341 ccgacgacga cgagctccgg cacatcgccg gtcgcggcga tcgtcgaggc cacgagatcg
     5401 gtgccgtcca cgaccgtcac cgggaagccg ctcatcgtca cggcaccgtc cggggaggtc
     5461 ccggtcgcgg cgatggccac ccggatgtcg ggacgttggg cgagggcggc ggcggcggcg
     5521 tcgatgcccg gatcgtccgc gcccccggcg tgcgtgagca gcacggtgag cgaggtggcg
     5581 gtgtgcggtg ccggtgggag ggtgtcccgt gcgctcgcca gctcggcgag ctccatcgtc
     5641 tcggccgcag ccgcatcggg cgccgcgctg tcggagccgc tgtccacgcc gcaggcactg
     5701 agcaccagca ccagtgcgac ggcacatccc ccgagccgga accgcatctg tgcgccagtg
     5761 tggacctgtc gcccgggcga tgggtggatg gtccgccggt acgctccgag accgtggaca
     5821 tcgcagcgtt ccaggccacc atcgccgcca cctacggcga gcgcgacgcc gatcggggca
     5881 ccgcgccgac ggtcgcgtgg ctgtgcgagg aggtgggcga gctggcccag gcggctcgca
     5941 agggcacgcc ggagcagcag ctccacgagc tgggcgacgt gatcgcctgg gtcgcgtccc
     6001 tcgcccacca gctcggactc agcctcgacg acgccgcgca gcgctacgca gccgggtgcc
     6061 cggtctgcgc gtccatgccc tgcgactgct gagcccggct cacggcccgc ggccgaccag
     6121 gtcgccgatg gccagcacga agccgggcgc ggcgacgagc acgacggcgc cgatggccag
     6181 gtgggcaacc cgcagcgcgg tcgggccggt cagcagcgac cggacccgct cgacgacgcg
     6241 gcctccgtac acgtaggcga ccccgcccac gagcgccgcg atcccgaacg ggaacagcac
     6301 gaccaggacg agcagcgacc cgtacgccaa cgatcgacgg tcacggccga tcagcgggag
     6361 ccaccggctg agccgggccg acacggcagc gagcgacacc ccgaacgcga gcgccagggc
     6421 ggagaacgcg gcgacggcga gccaccacgg cccgaccagg tcgaagtcct cgttgcccgg
     6481 tcgcagcggc tccatccggg tgccgaagac cacgagcagg ccggcaccga agaggaggcc
     6541 gccgatccag cgctccggga ggcagcggcg cacggcgaac cacagggtcg tcatcaccag
     6601 cccgctcagc aggccgatga agatgacgaa gccgatggtg ccgcccaccg tgatctcgcc
     6661 gacgacctcg ttggcctccg tgatccggcc ctgcgcggcg tcaccggcgg tggcgccgag
     6721 cagccgcatg gccaggcggc cgccgacgcc catcaccagc aacccgcaga gcagcgcggt
     6781 ggcgacggcg acctgcagcc accagaggta gtggcgagcg cgggcgcgcg gaccgtcctc
     6841 ggcctctggc gctgccggcg cggcgaagac ctcgtctcgc cagcgcgcca caccgaccaa
     6901 cgtcagcgcg aacagcgccc cgcacacgaa caccactgcc atggaccaca tggtggcgat
     6961 gagggcttgg aggacccttg gatcctccgt ggggcgtcag ctccggcggc ggagcagctc
     7021 ctcggcgatc tggatggtgt tcagcgccgc gcccttgcgg aggttgtcac ccgacaggaa
     7081 cagggccagg ccgtgctcga cggtgggatc gacgcggatg cggccgacga gggtgtcgtc
     7141 gatgccggcc gagtccagcg gcgtgggcac gtcggcgagc tggacgccgg cggcgtcggc
     7201 cagcagctcg cgggcccggt ccggggagat gggacgggag aagcgggcgt tgatcgacag
     7261 cgagtgcccg gtgaagaccg gcacgcgcac gcaggtgccc gagacgagca gctcggggag
     7321 gccgaggatc ttgcgcgact cgttgcgcag cttctgctcc tcgttggtct cctcgagccc
     7381 gtcgtcgacg aggtctccgg cgaaggggag cacgttgaag gcgatgggct tcacgaagtt
     7441 gtcgggctcg ccgaactcgg ccgcccggcc gtcgtgggtg aggccggggg cgtcgttgcc
     7501 gaccttgcgc acctgggcgt cgagctcctc gacgccggag aggccggcac cggagacggc
     7561 ctggtaggtg gagaccacga tggcttcgag gccggcctcg tcggccaggg gcttgagcac
     7621 cggcatggcg accatggtcg tgcagttcgg gttggcgacg atgccgatgg gcaggtcgtc
     7681 cagcgcgtcg gcgttcacct cgggcacgac gagcggcacg cgatcgtcca tccggaacgc
     7741 cgaggagttg tcgatgacga cagggccaga ggcggcgatg cgctcggcca gggcccggga
     7801 ggcggtggcg ccggcggagg ccagcaccac gtcgaggtcg gagtagtcgg cctcggtgga
     7861 gtcctcgacg gtgatctcgg tgtcacccca cggcagggtg cggcccgccg agcgagccga
     7921 ggcgaagtac cgcagctcgt cgaccgggaa gttccgttcc gccaggagct ggcgcatgac
     7981 gccgccgacc tgacccgttg ctccgaggat tcctacgcgc atggcaactc agggtagggg
     8041 cgtcgcccgg cgatgggaac ttcgttgccc gatcagcggg cgaggtaggt gatccgctgc
     8101 tgctggcgct gctcgatgcg gacgccgttg gcctccatca ccgcggtgcc ggcgatggtg
     8161 atgtccgagc ggggcacgat gccgtcggcg aggtcccact cgatggggcc gccgcccgtg
     8221 agctcaccgc tgatgacctc ggcagccacg cccatcacct gcacgttgcc gggcacgaac
     8281 cgcagggtct gctcgatggt tcccgccgcc cggtcctccc cgagctcggc gagcgacacg
     8341 accgtcgtga tctcgaccgg catgccggcg atctcggtct cggtgtcgac ccgccaccgg
     8401 gccccctggc cgatcgcctc gtcggggaag ggcaggctca tgctctcggc agagtcggtg
     8461 aaggagccgg cgagctgctc ggcgaacgcc ccggcggcgc cggggagctc cagcccctcg
     8521 agctcggcct cgacgatgcc gccccggctc gtgaacgtcg tgcgggctcg ggcatcgcgg
     8581 aagccctcca tgagcgatcg cagctcgtcg gcgacggccg ggtcgacgcc gggtgcgtcg
     8641 agcacgcgca cgtcgtcgta gacgccggtg gccgtgaacc ggtcaccgtc gacctcgtcg
     8701 atcgtgtagc tcacgtcgaa ctcgttcacg ggcgacggtg ccgactgcac ctggccgccg
     8761 gcatcgacct cgagtgacgt ctcctggcgg agggtcacct ggtcggtgtc gccggcgtcg
     8821 acgaccagcc ggagctcccg tcggggctcc tggcccgggt cctcgacggt gacccggtag
     8881 ggcgccgcgt cctcgtcgac cgggtcggcc gcctcggtgg tggtggacgt cgactcggtg
     8941 gtggtcgagg ggaccgcggt ggtcgtcgag gcggccgtgg tgtcgtcgtc gtcgccgcag
     9001 gcgacggcga ggacgaggag gagcggggcg aacgttcgga gcagggtgcg cacggaggac
     9061 gattcgccgg cggtgcgcgg attcctgcgc cgggctatgc gtcgaggtcg aaggccgtgt
     9121 gcaggacctg gacggcgtcg tccacctggg actgctggat cacgcaggag atgcggatgg
     9181 ccgacgtgga gatcatctcg atgttgatgt cgttgtcggc caggacctgg aacatcttgg
     9241 cggcgacgcc cgggttggac ttcatgccgg caccgacgac cgacacgcgg ccgatgtcgt
     9301 ggtcgaagcc gacgccggcg ttgccgacgt gacccgacag cgagttgatg agctcggtgg
     9361 cccgctcggc gtcggagtgc gggcaggtga aggagatgtc cgtgcggccg tggtcggaca
     9421 cgttctgcac gatcatgtcc acgttgacgc cggcgccggc gagcgacgtg aacatctgtg
     9481 cggcgacgcc cggctggtcg gacaccccgg agacggtgac cttggcctcg gaggtgtcgt
     9541 gggtgacggc gttgacgacg gcctgttcca tgtcggtggt ctcctcggtg acgatggtgc
     9601 ccggctccca cgtgaacgcg gaccggatgt ggagcttgac gtggtgccgg cgggcgaact
     9661 cgacggagcg catggccggc ttggggcagc ccgtggcggt catctcgagc agctcgtcga
     9721 agctgatctc ggccatgcgg cgggccttgg acacgatgcg cgggtcggcg gagtacacgc
     9781 cggcgacgtc ggtgtagagc tcgcagaact cggcgccgag tgcgtgggcg atggcgacgg
     9841 cggtggtgtc ggaaccaccc cgtccgagga aggtgacgtc gcgctcgatg gagacgccct
     9901 ggctgccgcc gatgaccggg atgaagccct cggccagcgc gtcggcgatg cgctccggcc
     9961 gcagctcgag gatcttggcg tccttgtggt tcgtgtcggt gatgaagccg gcctgcgagc
    10021 cggtgaacga ctgcgacggc acacccaggt cgctgatcgc catcgccacc agggccatgg
    10081 ccttgcgctc accggcggtg atgagcatgt ccatctcgcg acccggctgg gtcgtggaga
    10141 cgtcgtcggc gaggcggagc agcgcgtcgg tctccttgcc catggccgag accaccagca
    10201 cgacgtcggt gccgcggcgc ttcgtgcgca cggcgtgctc ggccacctcc cggatgcgct
    10261 ccgggtcggc caccgacgtg ccgccgaact tctggaccgt gatcacgtcg gccgaaccta
    10321 ccggcgcgac ccgagagcct cgaacttcag agcgtctcga ccagtgcgtc ccagtcgcgc
    10381 agctgctgca gggtctgggt ctcggtggag tgcagcagcg agcagatggc ccgcaggtcg
    10441 tcgcggcgga tcgacagcac gcgcccgttg aagtcgccgc gctcgaggat gatcgagcgg
    10501 gcgtagcggt ggaccgcctc ggcctcgggg atgccggaca gggccacgag gtcgaggatg
    10561 accttcgagg gccgatcgct gtcgtcgtcg tcgtcgttct cgccgagcag caccgacatc
    10621 ggcacgtcgt agaactcggc gagctcgcgc aggcggggca ggctcaggtt gcggaagccg
    10681 cgctcgtagg ccccgatggc cgacgcgctc cactgcccgc cgctctctcg ctcgacgtcg
    10741 tcgagcgaga gcttccgcag ccgccggacc gcgcgcaggc gctggccgac ctcgaccgag
    10801 aacgacgaga gactgtcgtc gtcgcttgcc atcgcacccc tcccgcactt cttccggccg
    10861 cgcgcacacg gatccgccct ctggtcgtac ggagcgtagc gaagggcgtg gcagaggtca
    10921 cgcgcggggc acagatggcc cggctgcgtg cggacggcca cgcgccgcgc ccggcacggc
    10981 gcgctcggtg tgactcgcgg ctcggcgggc gggacgggta cggtccgcgc ccgtgggtac
    11041 ttccaagcgc gagcgccaga agatggcgcg acacgagaag atcgaagagc tgcgccgcca
    11101 ggagcagcgc caggccaccc gcaagcgcgg ggtcctcatc ggcctgggca tcgccgcctt
    11161 cctggccttc gccatcggct actcggtgct gacctccgac gacgacggtg acgacgaggt
    11221 ggcgaccgcc gacgacaccg aggccgaggg cgccgaggac gagaccgcca cggaggacga
    11281 gacggccacc gaggacgacg gcaccaccga caccacggag ggcacctccg agaccaccga
    11341 gaccacggtg gccgaggtcc agcccggtga caccgagtgc ccgccggccg acgggtccgc
    11401 cgagcgccag atcgacttcg acggcccctt ccaggactgc ctcgaggacg gcgtggacta
    11461 ccaggcccgc ttcaccacca ccgagggcga ggtgctcgtc gacctgctcg aggagcagat
    11521 gccgaacacg gtgaacaact tcgtggccct ggcccgctac ggctactacg acgacaccct
    11581 gctgttccgc accgacccct cgatcgacat catccagggc ggctcgcccc acacgcagga
    11641 caacaccgac cagggaccgg gctacaacat ccccgacgag ggcggcgagt tcacgtccga
    11701 tgcggccacc ggccagtaca cggggccctt cacctacgag gagggccagc tgatcatggc
    11761 ccgctcgatg ggtccggact cctcgtcggc ccagttcttc ttcagcaccg gcccgaacgt
    11821 ggagctgctc gacagccagg gcacctacct gctgttcggt gacgtggtgg aggggctcga
    11881 cgtgctgcag gagatcatcg gcctgcacga ggacgacgac agcgggctcg gtggcgcacc
    11941 gagccgggac gtcacgatcg agtccgtgga gatcctggag ctctgagcgg cgcccgccgc
    12001 cgcgaccgct cggtccgcgg cagcgtgaac cggaacgtgg cgcccccacc gggggtctcg
    12061 tccacccaga tgcgcccgtc gaggcgctcg atcgactgct tggcgatgtg caacccgagc
    12121 cccacgccct gctgggagcg ggtcaggtgg ttgcccagcc gggtgaactt gtcgaacacc
    12181 cgggtccggt ccgagcgtgg gatccccggc ccgtggtcga tgacgctggt gatgagctcg
    12241 tcgccgtcgg cctccaccag gacctcgacg ccgccgtcgc cctcggcgta cttgagggcg
    12301 ttggacagca ggttggtgag gatgccgatg gccaggccct cgtcggccag caccgggccg
    12361 gcgtcggggt cgacgtcgag gtcgatggtg cgggatgggt cctggcgtcg gaccagctcg
    12421 acctgctcca ggacgcgatc ggcccagtcg acctcggtcc gctcgacgac catgccgccg
    12481 gcgtcgagct gggtggagcg caacagggag tcgaccaggg cctcgagccg ctgctcctcg
    12541 cggatcatga tctcgtagat gcgcgcccgg tcctcggtgg tgagctcggc atcgcggcgg
    12601 gcgagggtgt gcaggaaccc cttgatcggg gtcagcggcg tgcgcagctc gtgggagacc
    12661 tgggcgatcc agccgtcctt gtcgtcctgg agcttcttgc gggcggtctc gtccctggcc
    12721 acggccacgt agccgccgtc ggacagcggg gcgtaggagc acgtcagcca gcgctcctcg
    12781 tcgccggcct cgatccgcag cacgacgccg gtgaccggcc ggccgacgcg acccgggtcc
    12841 gccgaccccg tcgccggctc gccgctctcg gtcgtcggac ggaacgactg gctgacgtgc
    12901 tcgccgaccg cgacctcggc cgggacgccc gtgatcgtct ccatggccgg gttccacgcc
    12961 cgcacccgca ggtccgatcc gaccgagaag atgccgtcgg aggatgaccc gacgaggtcg
    13021 gcgagggtgc gtcgctcggt gctgacgtcg gtcagcagcg acagctggtg cagggtggtg
    13081 gagacccgct cggccagggc gtcgaggatg cgctcgtggc gggcggcgta ggggcggccg
    13141 tcggcccggg ccgagacggc gatggagccg cagggcaggc cggcgtcgtc gagcagccgc
    13201 atgcgcgacc cggagccgta cacgtcctcg gtggcggtgg ggccggccgc gacgcggacc
    13261 gtgtccccga tgccctcgat cagcacgacg gcgctgcggg ccccgaggag ggcgatggcg
    13321 tgctcggcga gctcgcgcgc cgcctcgtgg gtgtcggtgg cccgggacag cgcgagctcg
    13381 gcctctcgca accggtccag ctcgggggcg gtgagggtca cctggccgcg ccggcggacc
    13441 tcgaggccga cgagggcggc gacgacggtg gcgaccagcg cggtgagcat cgctccctgc
    13501 acgagcgcga cgacgacggc gaccacggcc agcacggccg cggcgccctg caggagcgcg
    13561 cctcgctggt gcggatcggg gaacctgttg gccatctgtc ctccgggcag ggtcgacgcc
    13621 gcgggtcgac ggggtgagtg tcgcgttcgt ccggcccgcc ccgccatgac ccgttcgggt
    13681 catcttcggt gcgaaaggga cgatcaggcg ggttgggcgg gcggcgccgg cggagtgccg
    13741 tcgcccggtg gcagcgcgtg gtgggcgacg ccctcgagca cctggcggag ccacagctgg
    13801 agggtcggga cccggtgccg gcggatgtcg atgaccgaca gaccggcggt gcgcagctcg
    13861 gccgggatgt cgcggtcggc gcgccagccg gtcagaccgc cgaggggcgg ggcgaccagg
    13921 cgctggaggc gaccgggcag gccgacctgg gccccgggct cgaggaacag gacccgcccg
    13981 tcgtcggcga ggacgcgccg cacggatcgc agggtctcgg tgaggtcggt gctggcggcg
    14041 agctggaaga ccgagacgac ggtgtcgaac cgctctccgg cttcggcgag ctcgccgagg
    14101 cggcggtcgc cgacgccgtc cagcaccgtc gtgtcggaga cgccgtcggc cccggtccac
    14161 aggttgcggt gcatgtcggc gccgcccagg tcgaggaggc ggccccgccc gtgccgggtc
    14221 agcccccgcc ggcgggcgcg cacgagcagc tgcgcctcgg gtgggagcgt gcgctcgtag
    14281 ccggtgggga tgatggcgct gtctgcgatg gggccagtct cgctttttcg ggcgcctggt
    14341 taccgctcgg tacggtgtga cgcatgacga tcaagagcgt gggcatcatc ggttcgggga
    14401 tcatggggtc cggcatcgca caggtcgcag cgacctccgg cttcgacgtg gtgctgcgct
    14461 cccgcaagca ggaatcggcc gagcagaccg tggccgccat gcagaagtcc ctcgagaagc
    14521 tcgtcgccaa ggagcggatg agccaggagg acgccgacgc ggccgtcgcc cgggtcaccg
    14581 ccacctcgaa gctcgacgac ctcgccgact gcgacctggt gatcgagtcc gtggtcgagg
    14641 acctcgccac caaggtggcc ctcttcaacg acctcgacca ccactgcaag gacgacgcca
    14701 tcctggcgac caacacctcc acgctgtcgg tgatcgagat ggcggtcgag acccgtcgcc
    14761 ccgagcgggt gtgcggcgtg cacttcttca acccggcccc gatgatgagc ctggtcgaga
    14821 tcatcaagac gctggtcacg tcggacgaga ccatcgccga ggtgcgccag ttcgccgagg
    14881 cctgcggcaa gtccccggtc gaggtcaagg accgcgccgg cttcatcgtg aaccacctgc
    14941 tgttcccgta cctgaacaac gcggtgcgga tgctggagaa cggcaccgcc agccgtgacg
    15001 acatcgactc ggccatgaag ggcggggcca acttcccgat gggtcccttc gccctgctcg
    15061 acctggtcgg cctcgacacc tcgctgtcga tcctcgacgc cctctacgac gagttccggg
    15121 acccgcacta cgcggcctcc ccgatgctgc gccgcatggt cagcgccggc cacctcggcc
    15181 gcaagtccgg cgccggcttc tacgagtact gatccggcgg cgggcccgac ccgcacccga
    15241 ggacgagcga aggccccggc gatgccgggg ccttcgtcgt cgtgcgtcct gctggtgggc
    15301 tgggtgcgac aactgccgtt ccatcccccg ttcgcgccgg ctcagaccgc gcggatgtga
    15361 cggtttcctt tcgatctggc cgaggcgccg gaacccgaat cggtcgacag cgcgggcgtc
    15421 ggggagaatc gacccgatgc ccgtcgagcc cccgccgtgc gcctggtcgt tcccggacgt
    15481 ggacacggcc gacgagtccg gcgtggtcgg cgtcggcggg gacctctcgc cgggcacgct
    15541 cctgcacgcc taccgcagcg ggctgttccc gatgcaggtg gaccggggcc gcacgctcgc
    15601 ctggtggtcg ccggaccccc ggggcgtcct cccgctcgac gggctgcggg tgtcccgctc
    15661 gctgcgccgg tcgtgcgccc gcttcgagat ccgggtcgac acttgcttcg acgaggtcgt
    15721 cgccagctgc gccgacccca agcgcccgca cggctggatc accgaggaga tccgccaggc
    15781 ctaccgccag atgcaccgcc tcggctgggc ccacagcgtt gaggcctggt cacgggagga
    15841 cgggacgctt gccgggggcc tctacggcgt ctcggtcggc ggcctgttcg ccggcgagtc
    15901 gatgttccac cgtgagcggg acgcctccaa ggtcgccctc gtcggcctgg tcgacatcct
    15961 ctccgccgac ggggtgaacc gcctgctcga cgtccagtgg accaccgacc acctccgctc
    16021 cctcggcgcg atcgacgtcc cccggaacga ctacatcgac aggctcagcg tcgcgctgcg
    16081 cctgccgatg ccgccggcct tcatctagtt ctggcagttg tggtgtagcg gctggatcag
    16141 cccttgccgc ccttgccctt tccggccggc gggccggcgt gggcgggctt gcccttgggc
    16201 tgctcctcgg tgaccgccgc agcagcaggc tcctggaccg gtgccgactg ctcggtcacc
    16261 gtcttctcgg tggtctctgc agccggctcc tcggcggtct tcttgccgat gtcggacttc
    16321 gcgacgccgg cgacgcactg acccttgtcc cggccgctca ggccggcgca ctcgccgccg
    16381 cccttggccg cctcggagac gtacttgccg tggttctcgg ggtgctcagc gacgacgtcg
    16441 tcgtcggtct cgtcatccac cagttcgtcg ttctccgcgc cctcgtcgtc ctcggggcag
    16501 gcctcgcctt cggcgggggt ggtgccgtcg tcgcagggct cggccggctt gggctcgtcc
    16561 tcggggcagg cctcgccttc ggcgggggtg gtgccgtcgt cgcagggctc gatcggcttg
    16621 ggctcgtcct ccggacagct gccgtcttcg tcgcggtcca caccgccttc acacggctcg
    16681 aggagggcgg ctgcaggatc gtcggtgggc gggtcgccct cgacggcgag ggcgagggtg
    16741 gcgccgccga tcacggtcac accggtgatg gcgacgatgg cggtcttctt ggcgaagagt
    16801 gcagcgaggg agcggatcat ggaccggaat ctcggcccgt cgtcagtcgt ccttgagggg
    16861 ccatttc
//
LOCUS       DATISU010000448         4846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_276368, whole genome shotgun sequence.
ACCESSION   DATISU010000448 DATISU010000000
VERSION     DATISU010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276368"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VLR27_17920"
     CDS             <1..360
                     /locus_tag="VLR27_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HSP05388.1"
                     /translation="AEVVGNRTRVKVVKNKVSPPFRQAEFDIMYGKGISREGSLLDIG
                     VDLGLVKKSGAWYTYEGEQLGQGRENAKTFLGENPEIMVEISERIRQQVGIDPGDEAE
                     AAVEDDIDLDAPISLDD"
     gene            468..725
                     /locus_tag="VLR27_17925"
                     /pseudo
     CDS             468..725
                     /locus_tag="VLR27_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
     gene            854..2269
                     /gene="miaB"
                     /locus_tag="VLR27_17930"
     CDS             854..2269
                     /gene="miaB"
                     /locus_tag="VLR27_17930"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HSP05389.1"
                     /translation="MTSRTYRVRTYGCQMNEHDSERIAGLFEADGLRPAADDEQPDVM
                     VLNTCCIRENADNKLYGNLGHLKSVKDANPGMEIVVAGCLAQKDRDLIQQRAPHVDVV
                     VGTHNVGRTVELLRTAREQGPITEIIEAVDADHAEFPSALPTKRELPYAAWVTIQIGC
                     DNSCAFCIVPSVRGPELSRPVDDIVAEVERLAADGVSEVTLLGQNVNSYGRDLTLAAR
                     KAGDDVRIRPLFADLLAATAGIEGIRRVRYTSPHPKDLRPETIAAMATHDEVCNHLHL
                     PLQSGSDAVLAAMHRGYTAERYLERVRAARAAIDDLALTTDIIVGFPGETDADFEATL
                     EVVAEAEYDSAYTFIFSPRPGTEAAELADRFVDPAVVGERYERLRRVVERSGLRKHEA
                     RIGRTEEVLVEGPSRKDPAVLTGRTAQNKLIHFPSPAPIRIGSYATVEVTGAAAHHLQ
                     GELRDIVHVAAHKTRIPVAAI"
     gene            2266..3189
                     /gene="miaA"
                     /locus_tag="VLR27_17935"
     CDS             2266..3189
                     /gene="miaA"
                     /locus_tag="VLR27_17935"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HSP05390.1"
                     /translation="MSGHGALVGPTASGKSTIAHAIARDDPTWELVTVDSMQVYRGMD
                     IGTAKPTPAEQAEVPHHLLDVADPWDDHDLATYQAAVREVLADIEARGRRALLVGGTA
                     LYLRVVVDDLTLPGQFPGVRAELEADVEVHGVAALHARLAELDPDAATRMEPTNHRRV
                     VRALEVTLGSGRPFSSFGPGLDAHPPTRFRLVGLRWDRSELDQRIADRYRSQLDAGFL
                     DEVRRLLDGPRPVSRSAAQALGYKELAAHLAGECTLEEAVDLAVRRTRRFARRQDRWF
                     RRDPRIEWVDVHGDPMDVVDQVREALERPAD"
     gene            3197..3994
                     /gene="dapF"
                     /locus_tag="VLR27_17940"
     CDS             3197..3994
                     /gene="dapF"
                     /locus_tag="VLR27_17940"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HSP05391.1"
                     /translation="MRLTKHHGLGNDFLVLLDRDGTQPIDEDIATAVCDRHRGVGADG
                     LIRVTTTTGEDGAARFAMELRNEDGSRAEMSGNGISCLGQAVVRAGLDADGVVEVDTD
                     AGPRTVTVVETQAPHRHRMRVAMGAARLGDELPEWVDQRILRALHVDMGNPHVVLHVA
                     DAADIPDLVEIGERINSSTPGGTNVELVLPGDPGEIRMLVYERGVGPTQACGTGAVAA
                     AAAAHAWELAPTTVTVHQPGGPAVVELDDVAHLTVPVVAIAAVEWPL"
     gene            4011..>4846
                     /gene="hflX"
                     /locus_tag="VLR27_17945"
     CDS             4011..>4846
                     /gene="hflX"
                     /locus_tag="VLR27_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HSP05392.1"
                     /translation="MTDALTDTLIDRSFREKIVLVGVTLPPDDPEETERGLDELSALI
                     DTAGADEVARVVQRRQAPDPPTFIGKGKAEELLEVALATDCDTVVFDNELTPAQQCNL
                     EKMLGRTAIDRTAVILDIFAQNAASVEGKAQVELALLRYRLPRLRRTGKSFSQQAGGM
                     SAAGGARIGSRGPGETQLETDRRRLVRRIHRLESDLKEIQQHRDTQRKRRKSARTQRV
                     AIVGYTNAGKSTLLNRLTDAGVLVEDRLFATLDATTRRLDLPGGESVLATDTVGFVRK
                     LP"
BASE COUNT          741 a         1880 c         1633 g          592 t
ORIGIN      
        1 gccgaggtcg tgggcaaccg cacccgggtg aaggtggtca agaacaaggt ctccccgccg
       61 ttccgccagg ccgagttcga catcatgtac ggcaagggca tcagccgcga gggctcgctg
      121 ctcgacatcg gcgtcgacct cggcctcgtg aagaagtccg gcgcttggta cacctacgag
      181 ggcgagcagc tcggccaggg ccgggagaac gccaagacct tcctcggtga gaaccccgag
      241 atcatggtcg agatctccga gcggatccgc cagcaggtcg ggatcgaccc gggcgacgag
      301 gccgaggccg ccgtcgagga cgacatcgac ctcgacgccc ccatctccct cgacgactga
      361 ggacgcgccg cccctcccgg ccgggccggt cccggccggg aggagagtcc tcggtgcgcg
      421 tcgaagaagg cgggcactgg ggatcacggc ctctcgaagg cggcggaatg gaacagctca
      481 aggtctcgac gagatcgaac ccgaactcgg tggccggtgc catggcgggc gtcgtgcgct
      541 cgcagggcgc ggtcgaggtg caggtcgtcg gcgccggcgc cctcaaccag gcgatcaagg
      601 cggtggccat cgcctgcggc tacgtggcgg ccagcggcat cgagctggtg tgcatccccg
      661 aattcgccga catcaccatc gacggcgaga agcgcaccgc catccggctg cgcgtcgagg
      721 accgcgtccc ggtccccgac ggggacgacg tggtggccct cgacgatccg gtcgccgagg
      781 ccgccatcgc cctcgacggc tgagctcgtc gccttcagga cccgccgggc ggaggcgtag
      841 cctcggggtc gccatgacgt ctcgcaccta ccgcgtccgc acctacgggt gccagatgaa
      901 cgagcacgac tccgagcgca tcgccggcct gttcgaggcc gacgggctgc gcccggccgc
      961 ggacgacgag cagcccgacg tcatggtgct caacacgtgc tgcatccggg agaacgccga
     1021 caacaagctc tacggcaacc tcggccacct gaagtcggtc aaggacgcca accccggcat
     1081 ggagatcgtc gtcgccggct gcctggccca gaaggaccgg gacctcatcc agcagcgcgc
     1141 cccgcacgtc gacgtcgtcg tcggcaccca caacgtgggc cgcaccgtcg agctgctgcg
     1201 caccgcccgg gagcagggac ccatcaccga gatcatcgag gcggtcgacg ccgaccacgc
     1261 cgagttcccg agcgccctgc ccaccaagag ggagctgccc tacgccgcct gggtgacgat
     1321 ccagatcggc tgcgacaaca gctgcgcgtt ctgcatcgtg ccgtcggtcc gcggccccga
     1381 gctcagccgg cccgtcgacg acatcgtcgc cgaggtcgag cgcctcgccg ccgacggggt
     1441 cagcgaggtc accctcctcg gccagaacgt gaactcctac gggcgcgacc tcaccctcgc
     1501 cgcccgcaag gccggcgacg acgtgcgcat ccggccgctg ttcgccgacc tgctcgccgc
     1561 caccgccggc atcgagggca tccggcgggt ccgctacacc agcccgcacc ccaaggacct
     1621 gcgccccgag accatcgcgg ccatggccac ccacgacgag gtctgcaacc acctccacct
     1681 gccgctgcag tccggctccg atgcggtgtt ggccgccatg caccggggct acacggccga
     1741 gcgctacctc gagcgggtgc gcgccgcccg ggccgccatc gacgacctgg ccctcaccac
     1801 cgacatcatc gtcggcttcc ccggcgagac cgacgccgac ttcgaagcca ccctcgaggt
     1861 cgtcgccgag gccgagtacg actcggccta caccttcatc ttctcgcccc gcccgggcac
     1921 cgaggccgcc gagctcgccg accgcttcgt cgaccccgcc gtggtggggg agcgctacga
     1981 gcggctgcgc cgggtggtcg agcgctccgg cctgcgcaag cacgaggcgc gcatcggccg
     2041 caccgaggag gtgctggtcg agggtccgag ccgcaaggac ccggccgtgc tcaccgggcg
     2101 caccgcccag aacaagctga tccacttccc gtcgccggcc ccgatccgca tcggctccta
     2161 cgccacggtc gaggtcacgg gcgcagcggc ccaccacctc cagggcgagc tgcgcgacat
     2221 cgtgcacgtc gccgcccaca agacccgcat cccggtggca gcgatctgag cggtcacggc
     2281 gccctcgtcg ggcccacggc atcgggcaag tccacgatcg cccacgccat cgcccgggac
     2341 gaccccacgt gggagctcgt cacggtcgac tccatgcagg tgtaccgcgg catggacatc
     2401 ggcacggcca agcccacgcc ggccgagcag gccgaggtgc cccaccacct gctcgacgtg
     2461 gccgacccgt gggacgacca cgacctggcg acctaccagg ccgcggttcg ggaggtgctg
     2521 gccgacatcg aggcccgggg tcgtcgggcg ctgctcgtgg gcggcacggc gctgtacctg
     2581 cgggtggtcg tcgacgacct cacgctgccc gggcagttcc ccggtgtgcg ggccgagctc
     2641 gaggccgacg tcgaggtgca cggcgtggcg gcgctgcacg cccgcctcgc cgagctcgac
     2701 cccgatgccg ccactcggat ggaaccgacg aaccaccggc gcgtggtgcg cgccctggag
     2761 gtgacgctcg gcagcggccg accgttctcc tccttcggac cgggcctcga cgcccacccg
     2821 cccacccgat tccgactggt cgggctgcgc tgggaccgca gcgagctcga ccagcgcatc
     2881 gccgaccgct accggtccca gctcgatgcc gggttcctcg acgaggtccg ccggctcctg
     2941 gacggccccc gacccgtgtc gcgctccgcg gcccaggccc tcggctacaa ggagctggcc
     3001 gcccacctcg ccggcgagtg caccctcgag gaggccgtcg acctggccgt gcgccgcacc
     3061 cgccgcttcg cccgccgcca ggaccgctgg ttccggcggg acccccgcat cgagtgggtc
     3121 gacgtccacg gcgatccgat ggacgtggtc gaccaggtgc gtgaggcgct cgagcgcccg
     3181 gcagactgag cgggacatgc gcctgaccaa gcaccacggc ctcggcaacg acttcctcgt
     3241 cctgctcgac cgcgacggca cccagcccat cgacgaggac atcgcgacag cggtgtgcga
     3301 tcgccaccgg ggcgtcggcg ccgacgggct gatccgggtc accacgacca cgggtgagga
     3361 cggcgccgcc cgcttcgcca tggagctgcg caacgaggac ggctcccggg ccgagatgag
     3421 cggcaacggc atctcctgcc tgggccaggc cgtggtccgg gcgggactcg acgccgacgg
     3481 cgtggtcgag gtcgacaccg acgccggccc ccgcaccgtc accgtcgtgg agacccaggc
     3541 gccgcaccgg caccggatgc gggtggccat gggcgccgcc cgcctcggcg acgagctgcc
     3601 cgaatgggtg gaccagcgca tcctgcgggc cctgcacgtc gacatgggca acccccacgt
     3661 ggtcctccac gtcgccgacg ccgccgacat ccccgacctg gtcgagatcg gcgagcggat
     3721 caacagctcc acgcccgggg gcaccaacgt cgagctcgtc ctgcccggag accccggcga
     3781 gatccggatg ctggtctacg agcggggcgt cggcccgacc caggcctgcg gcaccggcgc
     3841 cgtcgccgcc gcagccgccg cccacgcctg ggagctggcg cccacgaccg tgaccgtgca
     3901 ccagccgggc ggcccggccg tggtggagct cgacgacgtc gcccacctca ccgtgccggt
     3961 cgtggcaatc gctgccgtgg agtggccgct gtgatgggag gatgggcacc ttgaccgacg
     4021 ccctcaccga cacactcatc gaccggtcgt tccgggagaa gatcgtcctg gtgggcgtca
     4081 cgctgccgcc ggacgacccg gaggagaccg agcggggcct cgacgagctc agcgcgctca
     4141 tcgacaccgc cggcgccgac gaggtcgctc gcgtcgtgca gcgccggcag gccccggacc
     4201 cgcccacctt catcggcaag ggcaaggccg aggagctgct cgaggtcgcg ctcgccaccg
     4261 actgcgacac ggtcgtgttc gacaacgagc tcaccccggc ccagcagtgc aacctggaga
     4321 agatgctggg gcgcacggcc atcgatcgca ccgccgtgat cctcgacatc ttcgcccaga
     4381 acgcggccag cgttgagggc aaggcccagg tcgagctggc cctgctgcgc taccggctgc
     4441 cccgcctgcg ccgcaccggc aagtcgttct cccagcaggc aggcggcatg tccgcggccg
     4501 gcggcgcccg catcggttcc cgtggtcctg gtgagaccca gctcgagacc gaccggcggc
     4561 gcctggtgcg ccggatccac cggctcgagt ccgacctgaa ggagatccag cagcaccgcg
     4621 acacccagcg caagcggcgc aagagcgccc gcacccagcg ggtcgccatc gtcggctaca
     4681 ccaacgccgg caagtccacc ctcctcaacc gcctcaccga tgccggcgtg ctggtcgagg
     4741 accgcctctt cgccaccctc gacgccacca cccgccgcct cgacctgccc ggtggcgagt
     4801 ccgtgctggc caccgacacc gtcggcttcg tgcgcaagct gcccca
//
LOCUS       DATISU010000449         7631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_696459, whole genome shotgun sequence.
ACCESSION   DATISU010000449 DATISU010000000
VERSION     DATISU010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696459"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2275
                     /locus_tag="VLR27_17950"
     CDS             <1..2275
                     /locus_tag="VLR27_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HSP05393.1"
                     /translation="IPPDEIRDIVLGQLPNTAMFASRFRECAGRALLLPRRRPDRRQP
                     LWQQRQRAADLLKVAAKYPTFPILLETTRECVNDVFDLPALEEVLRDIRNRRVRVVPV
                     ETSQASPMAQSLLFGWIAVYMYEGDTPLAERRAAALALDRDLLRELLGSEELRELIDP
                     EVLADLELELQRLVEGRRARDADEVHDLLRLLGPLTLEEIDIRCEGDASALVSALADD
                     RRVYVASVAGEDRWAITEDAGRLRDALGVTVPVGLPAAFTDPVDDPMADLVARYARTH
                     APFTAASLAARYGISVERVLPSLERLEADGRVLRGEFRPDGHDREWTDPDVLRTLRRR
                     SLAALRKEVEPVESDALGRFLPAWHDIGRQRRGVDALIDTVSQLQGAPIPASVLETDI
                     LPARLPEYDPSQLDQLCAAGEVVWVGSGSLGAKDGRIRLLFRDHAPLLVPTLDPAPDG
                     EAHRAILDHLDQRGASFWTDIVAACQQAGAPYDEATVLAALWDLVWAGYVTNDGLGPL
                     RSFLGGAPRRGSTNKGRSRPRPGRLVRLGPAAGAGRWSLVAPLLQPTPTPTEAAHAKA
                     RQLLERYGVLTREAALGEGSEGGFAGVYPVLKALEERGEVRRGYFVSGLGAAQFAMGG
                     AVDRLRSSRSVERDETGQGLVLAGTDPAQPYGAALPWPESAGRPARQAGAHVVLVDGE
                     LVAFLERGGHSLSTFATTAEHPEWAELLKGLVRRGRYRSLEIRKVDGEPVRESPVREV
                     LEAAGYKDGYKGLVFRS"
     gene            complement(2272..2889)
                     /locus_tag="VLR27_17955"
     CDS             complement(2272..2889)
                     /locus_tag="VLR27_17955"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSP05394.1"
                     /translation="MTTRRRGRPRRTPAERAAQRDRLVEAAMQTVRERGPDVSMDHLA
                     KGAGVSKPVLYSEFGDKRGVADAIAVTLARRLEASAAADLADQPMEIPSVVRATISAL
                     IDLIDTEPELYSFIVRAIRTGDRGLLDNGLVQVIQERLAVLMALLLPGLDTVRLRALS
                     DGLFGFGFAAVESWTSHRELSKEELIDALSVVVQAGIEAAGTLRD"
     gene            complement(2959..3789)
                     /locus_tag="VLR27_17960"
     CDS             complement(2959..3789)
                     /locus_tag="VLR27_17960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HSP05395.1"
                     /translation="MTTTTPIPARDITVRRPGIDHEQPLPKHFVADDPMRSHIAAMLS
                     SVFPEGEDFFVRSVRAYRDRIDDPELSKQVKGFIGQEAIHGREHRDFNEQLAELGYPT
                     LAVDGIIKRFLAFLNKTTPKSHQLAVTAALEHYTATLAEALLRDDDARAEFATDEVRA
                     LFTWHALEETEHKSVAFDVYEEVSGNYIERVATMFAVHVAFLGIMTGAVTVSVAKDRS
                     AWRKLPKSILGLRTHPFFQRHVVSRLLDYNRKGFHPDDHDTVELLEEWRTRYEAGIAA
                     "
     gene            3926..4642
                     /locus_tag="VLR27_17965"
     CDS             3926..4642
                     /locus_tag="VLR27_17965"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP05396.1"
                     /translation="MPHPAEVDERLRAHLRSWLGAWPPARPVHVVGSERRVEPGWDGA
                     VRALNGVATPDGAVVSVPPAAEARIATVVEELEADGVADVLAALGERLADLLDLPGAR
                     YGAGVFRWSTEPTASDDPGTWLPRSDPRVPEWLHPFNGDVLVALADDGSVAAGVGRKQ
                     HDPHGHELAVVTEEAHRGQGFARRLVTQAARRVLADDAVPVYLHAESNVASARTADAS
                     GFPDVGWRILGLFGGAAGTP"
     gene            4644..5438
                     /locus_tag="VLR27_17970"
     CDS             4644..5438
                     /locus_tag="VLR27_17970"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HSP05397.1"
                     /translation="MPEGDTIHRAAIRMRPALAGHVLTRFEAPRLVGDRPALGERIDD
                     VEARGKHLLVHFSGGLTLRTHMKMTGSWHLYRDGERWRKGAHLARAVVGADGGWLAVC
                     FQAPVVETYHRAAGEPLPLASLGPDLTGPDPDLDAAIARAALAAPGTQLADALLDQRI
                     ASGIGNVYKSEVCFLHGLDPFTPLAEVPDDVLRRVLDTSHRLLVANLGRTRRVTYRDG
                     VAVYGRRGQPCFRCGSVIRMRRQGEMARSTYWCPTCQPAYAPTTPT"
     gene            5550..6425
                     /locus_tag="VLR27_17975"
     CDS             5550..6425
                     /locus_tag="VLR27_17975"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HSP05398.1"
                     /translation="MSTWQARGPRTLERTQVYDLDSSVLGERLQVTVAEPPLAGDGRE
                     PLATLVVLDPMATFDVVVGITRSLSLLAFGAFPPLLVVGVGYDADPLTTMSLRFRDLT
                     PTDAPIPELLAPPVPPAHGAGGADRFLDAIASDVLPHVASEHRGDVSDVTIVGWSLAG
                     LFGCHALLTRPEVFRRYLLASPSIWWDDAGILALEERTAATREHLDAHVYLAVGEREE
                     VAETRAWPVAMPPAAVSAAAMVTNAERLVRRLRSRGTKGLDVHLEVLADEHHTSIFPS
                     AASRGLLHLFGPSAA"
     gene            6522..>7631
                     /locus_tag="VLR27_17980"
     CDS             6522..>7631
                     /locus_tag="VLR27_17980"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSP05399.1"
                     /translation="MQDGSQRPKVVVVGAGFGGLSVLRGLADQPVDVTVVDRNNFHTF
                     LPLLYQVATSGLNAADVAHPIRAIVARHRNIDVRHGTVEGVDWDRHEVRVAGQAPLPF
                     DHLVLAAGSTANDFGIPGVAEHGYPLYTLDDAARLRNRVLERFEAAALDQSLIDDGAL
                     TFAIVGGGPTGVETAGALAELFDKVLRDDFHTLPVQRARVVLIEMADSLLTPFGTRSR
                     DHALDALRRRGVEVRLSTRVSSVGDDHVVLDGGERLPAHTLVWAAGVQANPLAGVLGV
                     AQGPGGRIVVDRDLSVPERPGLWVIGDLAHTPGAVEGGGAAPQLAPAAMQAGELVAAN
                     VLRTVAGEPTQPFRYRDKGTMATIGRRSAVAEIPGV"
BASE COUNT          962 a         2842 c         2735 g         1092 t
ORIGIN      
        1 catcccgccc gacgagatcc gcgacatcgt cctcggccag ctgccgaaca cggcgatgtt
       61 cgcgtcccgc ttccgcgagt gcgccggccg tgccctgctc ctgccccgcc gccggcccga
      121 ccgccgccag ccgctgtggc aacagcgcca gcgggccgcc gacctgctga aggtggcggc
      181 caagtacccg acgttcccga tcctgctcga gaccacgcgc gagtgcgtca acgacgtgtt
      241 cgacctaccg gcgctcgagg aggtcctgcg cgacatccgc aaccgccggg tgcgggtcgt
      301 gccggtggag accagccagg cgtcgccgat ggcgcagtcg ctgctcttcg gctggatcgc
      361 cgtctacatg tacgagggcg acacgccgct ggccgagcgc cgggccgccg cgctggcgct
      421 cgaccgggac ctgctgcgcg agctgctcgg ctcggaggag ctgcgcgagc tcatcgaccc
      481 cgaggtcctg gccgacctcg agctcgagct gcagcgcctc gtcgagggac gtcgcgcccg
      541 cgacgccgat gaggtccacg acctgctccg tctgctcggt cccctcaccc tggaggagat
      601 cgacatccgc tgcgagggcg atgcgtccgc gctcgtctcg gcgctggccg acgaccggcg
      661 ggtctacgtg gcgtcggtcg ccggcgagga ccggtgggcc atcaccgagg acgccggccg
      721 cctgcgcgac gccctcgggg tcaccgtccc cgttggcctc ccggccgcgt tcaccgaccc
      781 ggtcgacgat ccgatggccg acctcgtggc ccgctacgcc cgcacgcacg cccccttcac
      841 ggccgcgtcg ctggctgcgc gctacggcat cagcgtcgag cgtgtgctgc cgtcgctcga
      901 gcgcctggag gccgatggtc gcgtgctccg cggcgagttc cggcccgacg gccacgaccg
      961 cgagtggacc gaccccgacg tgctgcgcac gttgcgccgg cgctccctcg ccgcactgcg
     1021 gaaggaggtg gagccggtcg agtccgacgc gctcggccgc ttcctgcccg cctggcacga
     1081 catcggacgg cagcgccggg gcgtcgacgc gctcatcgac acggtctccc agctgcaggg
     1141 cgcgccaatc cccgcgtcgg tgctggagac ggacatcctc ccggcccggc tccccgagta
     1201 cgacccgtcc cagctcgacc agctctgcgc cgccggtgag gtcgtgtggg tcggcagcgg
     1261 cagcctcggc gccaaggacg gccgcatccg cctgctgttc cgggaccacg ccccgctgct
     1321 cgtcccgacc ctggaccccg ccccggatgg cgaggcccac cgggcgatcc tcgaccacct
     1381 cgaccagcgc ggcgccagct tctggaccga catcgtcgcg gcgtgccagc aggccggtgc
     1441 gccctacgac gaggcgaccg tgctcgccgc gctgtgggac ctggtgtggg ctggctacgt
     1501 aaccaacgat ggcctcggcc cgctgcgctc gttcctcggg ggcgcaccac ggcgggggag
     1561 cacgaacaag ggtcgctcac gcccccgacc cggacggctg gtccggctcg ggcctgcggc
     1621 cggcgctggt cgctggtcgc tggtcgcacc gctgctccag ccgacaccga cgcccaccga
     1681 ggccgcccac gccaaggccc gccagctgct cgagcgctac ggcgtgctca cccgcgaggc
     1741 cgcgctcggt gagggcagcg agggcggctt cgccggcgtg tatccggtgc tcaaggcgct
     1801 ggaggagcgc ggcgaggtcc gtcggggcta cttcgtctcc ggcctcggcg ccgcccagtt
     1861 cgccatgggt ggtgcggtcg accggttgcg ctcgtctcgc tcggtggagc gggacgagac
     1921 cggtcagggc ctggtcctcg ccggcaccga ccccgcccag ccctacggtg ccgcgctccc
     1981 gtggcccgag tccgccggtc gcccggcccg ccaggccggc gcgcacgtcg tgctggtcga
     2041 cggcgagctg gtggcgttcc tcgagcgggg cgggcactcg ctgtcgacct tcgccaccac
     2101 ggccgagcac ccggagtggg ccgagctgct caagggcctg gtccggcgcg ggcgctaccg
     2161 cagcctcgag atccgcaagg tcgacggcga gccggtccgg gagtcgccag tgcgcgaggt
     2221 gctggaagcc gccggctaca aggacggcta caagggcctc gtcttccgca gctagtcccg
     2281 cagcgtgccg gcggcctcga tgccggcctg gacgacgacg gacagggcgt cgatcagctc
     2341 ctccttggac agctcacgat gcgaggtcca cgactccacg gcggcgaagc cgaagccgaa
     2401 caggccgtcg gacagcgccc gcagccgcac ggtgtcgagg cccggcagca gcagcgccat
     2461 cagcacggcg aggcgctcct ggatgacctg caccagcccg ttgtcgagca gcccccggtc
     2521 gccggtgcgg atggcccgga cgatgaacga gtagagctcg ggctcggtgt cgatgaggtc
     2581 gatgagggcg ctgatcgtgg cccgcaccac ggaggggatc tccatgggct ggtcggcgag
     2641 gtcggccgcg gcgctggcct cgaggcggcg ggccagggtc accgcgatgg cgtcggccac
     2701 gccgcgcttg tcaccgaact cgctgtagag caccggcttg ctgacgccgg cgcccttggc
     2761 gaggtggtcc atcgagacgt cgggcccgcg ctcgcggacg gtctgcatcg cggcctcgac
     2821 caggcggtcg cgctgcgcgg cgcgctcggc gggggtgcgg cggggccgac cccggcgacg
     2881 ggtggtcacc gtccgcttcc ttgcgcgcgc atcgaccaag gatggtcgat ccgcgcgcag
     2941 ggaacgagtt ctcgccggtc aggcggcgat gccggcctcg tagcgggtgc gccactcctc
     3001 gaggagctcg acggtgtcgt ggtcgtcggg gtggaagccc ttgcggttgt agtcgagcag
     3061 ccggctgacg acgtgacgct ggaagaacgg gtgcgtccgc aggccgagga tgctcttggg
     3121 cagcttgcgc cacgccgagc ggtccttggc gaccgagacg gtgacggcgc cggtcatgat
     3181 cccgaggaag gccacgtgga cggcgaacat cgtggccacc cgctcgatgt agttgccgct
     3241 gacctcctcg tagacgtcga aggcgaccga cttgtgctcg gtctcctcga gggcgtgcca
     3301 ggtgaacagg gcccgcacct cgtcggtggc gaactcggcc cgggcgtcgt cgtcgcgcag
     3361 cagagcctcg gccagggtgg ccgtgtagtg ctcgagcgct gccgtgacgg cgagctggtg
     3421 gctcttcggc gtcgtcttgt tcaggaaggc gaggaagcgc ttgatgatgc cgtcgacggc
     3481 gagcgtgggg tagcccagct cggccagctg ctcgttgaag tcgcgatgct cacggccgtg
     3541 gatggcctcc tggccgatga agcccttgac ctgcttggac agctccgggt cgtcgatgcg
     3601 gtcgcggtag gcccggacgc tgcgcacgaa gaagtcctca ccttcgggga agaccgagga
     3661 gagcatggcg gcgatgtggc tgcgcatcgg atcgtcggcc acgaagtgct tggggagggg
     3721 ctgctcgtgg tcgatgccgg gtcggcggac ggtgatgtct cgggcgggga tcggggtcgt
     3781 ggtggtcatc agtcgtgctc ccgggggtgg aggacaagga attggctact gggcgtagga
     3841 taaatgcttc ggtccggttc gtaaacggtc tgtgcccaga tatcggtgtg acatgtaccg
     3901 ctggtaaccc cagtagcgtg agcgggtgcc gcaccccgcc gaggtcgacg agcgcctccg
     3961 ggcgcacctg cgctcgtggc tcggtgcctg gccgccggca cggccggtgc acgtcgtggg
     4021 ctccgagcgc cgggtcgagc cggggtggga cggcgccgtc cgtgccctga acggcgtggc
     4081 cacgcccgat ggggccgtgg tctccgtgcc gccggccgcc gaggcgcgga tcgccaccgt
     4141 ggtcgaggag ctggaggccg acggggtcgc tgatgtgctg gccgccctcg gcgagcgcct
     4201 cgccgacctg ctcgacctcc ccggcgcccg gtacggcgca ggggtcttcc gctggtcgac
     4261 cgagcccacg gcatccgacg accccggcac gtggctgccc cgcagcgatc cccgggtgcc
     4321 cgagtggctc cacccgttca acggcgacgt gctggtcgcg ctcgccgacg acggctcggt
     4381 ggcggcaggc gtcggtcgca agcagcacga cccccacggc catgagctgg ccgtggtcac
     4441 cgaggaggcc caccgcggcc agggcttcgc ccgacgactc gtcacccagg ccgcccgacg
     4501 ggtgctcgcc gacgacgccg taccggtgta cctgcacgcc gagagcaacg tcgcctccgc
     4561 ccgcaccgcc gatgccagcg ggttccccga cgtgggctgg cgcatcctcg gcctcttcgg
     4621 cggcgccgcc gggaccccct gacgtgcccg agggcgacac gatccaccgg gccgccatcc
     4681 ggatgcgccc ggcgctggcc ggccacgtgc tcacccgctt cgaagcgccc cggctggtgg
     4741 gggaccggcc ggccctcggc gagcggatcg acgacgtgga ggctcggggc aagcacctgc
     4801 tcgtccactt cagcggcggc ctcaccctcc gcacccacat gaagatgacg gggtcctggc
     4861 acctgtaccg cgacggtgag cgctggcgga agggtgccca cctcgcccga gcggtggtcg
     4921 gcgccgacgg gggttggctc gccgtgtgct tccaggcgcc ggtggtcgag acctaccacc
     4981 gcgccgccgg cgagccgctc ccgctcgcca gcctgggacc cgacctcacc ggacccgacc
     5041 cggacctcga cgccgccatc gcccgagccg cgctcgccgc acccggcacc cagctcgccg
     5101 acgccctcct cgaccagcgc atcgcatcgg ggatcggcaa cgtctacaag tccgaggtct
     5161 gcttcctgca cggcctcgac ccgttcaccc cgctggccga ggtgccggac gacgtgctcc
     5221 ggcgggtgct ggacacctcc catcggctgt tggtcgccaa cctcggccgc acccggcggg
     5281 tgacctaccg cgatggcgtc gccgtctacg gccggcgggg ccagccctgc ttccggtgcg
     5341 gttcggtgat ccgcatgcgc cgccaggggg agatggcccg ctccacctac tggtgcccca
     5401 cctgccagcc ggcctacgcc ccgaccaccc ccacctgacc gttctggcct acatccgatc
     5461 cgccatgcgg ggttcgatgt aggccagaac gaggtggggt cggccgcgga cgcccgacgg
     5521 cgtgcgaaga gctgtcgtac gatccgactg tgagcacgtg gcaggcccgt gggcctcgga
     5581 cgctcgagcg cacccaggtg tacgacctcg attcgagcgt gctcggtgag cggctgcagg
     5641 tgacggtggc agagccgccg ctcgccggtg acggacgcga gcccctggcg acgctcgtcg
     5701 tgctcgaccc catggcgacc ttcgacgtgg tcgtggggat cacgcggtcg ttgagcctgc
     5761 tcgccttcgg ggcgttcccc ccgttgctgg tcgtgggcgt gggctacgac gccgatccgc
     5821 tcacgacgat gtcgctgcgc ttccgggacc tcacgccgac cgacgcgccg atccccgagc
     5881 tgctcgcgcc tcccgttccg ccggcgcacg gtgccggcgg tgccgatcgg ttcctcgacg
     5941 ccatcgccag cgacgtcctg ccgcacgtcg catccgagca ccgcggggat gtgtccgatg
     6001 tgaccatcgt cggctggtcc ctcgccggtc tcttcgggtg ccacgccctg ctgacccgcc
     6061 ccgaggtctt ccggcgctac ctcctcgcca gcccgtcgat ctggtgggac gacgccggga
     6121 tcctcgcgct cgaggagcgc accgccgcga cccgtgagca cctcgacgcg cacgtgtacc
     6181 tcgccgtcgg cgagcgcgag gaggtggccg agacccgggc gtggccggtc gccatgccgc
     6241 ccgctgcggt ctccgctgcg gcgatggtca ccaacgccga gcggctggtc cggcgactcc
     6301 gcagccgggg gacgaagggg ctcgacgtcc acctggaggt gctcgctgac gagcaccaca
     6361 cgtcgatctt ccccagcgcc gcgagccgcg gcctgctcca cctcttcggt ccgtccgccg
     6421 cctgatccgc ggcccgcacg aaaccgcgtg cgggaccggg catgatccgg cacacttcgt
     6481 gaaagcattc acgaagtccg gtcacccgaa ggaggggcca gatgcaggac ggttcccagc
     6541 ggccgaaggt cgtcgtggtc ggtgccgggt tcggtgggtt gtccgtgctg cggggcctgg
     6601 ccgaccagcc cgtcgacgtc accgtggtgg accgcaacaa cttccacacc ttcctaccgc
     6661 tgctgtacca ggtggccacc agcgggctga acgctgccga cgtcgcccac ccgatccggg
     6721 ccatcgtcgc tcgccaccgc aacatcgacg tccgccacgg caccgtcgag ggcgtggact
     6781 gggaccgcca cgaggtgcgc gtcgccggcc aggccccgct cccgttcgac cacctcgtgc
     6841 ttgcggccgg ctccaccgcc aacgacttcg gcatccccgg cgtggccgag cacggctacc
     6901 cgctctacac gctcgacgac gcggcccggc tgcggaaccg cgtgctggag cgcttcgagg
     6961 ccgccgcctt ggaccagtcc ctcatcgacg acggcgccct caccttcgcc atcgtcggcg
     7021 gcggaccgac cggggtcgag accgccggcg ccctggccga gctgttcgac aaggtcctgc
     7081 gcgacgactt ccacaccctt ccggtgcagc gggcccgggt cgtgctcatc gagatggccg
     7141 actcgctgct gacgccgttc ggcacccgca gccgtgacca cgccctcgac gcgctccgtc
     7201 gccggggcgt ggaggtccgg ctgtcgaccc gggtgtcctc ggtcggggac gaccacgtcg
     7261 tgctcgacgg cggcgagcga ctccccgccc acacgctggt gtgggcggcg ggcgtccagg
     7321 ccaacccgct ggccggcgtc ctcggggtcg cccagggtcc gggcggtcgc atcgtcgtgg
     7381 atcgcgacct gtcggtgccg gagcgccccg gcctgtgggt gatcggcgac ctcgcccaca
     7441 cgccaggtgc cgtcgagggc ggtggcgccg caccccagct ggcgcccgcg gccatgcagg
     7501 ccggcgagct ggtcgccgcc aacgtcctgc gcacggtcgc cggcgagccg acccagccgt
     7561 tccgctaccg cgacaagggc accatggcca ccatcggtcg ccgctcggcc gtggcggaga
     7621 tccccggcgt g
//
LOCUS       DATISU010000450         5065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_276838, whole genome shotgun sequence.
ACCESSION   DATISU010000450 DATISU010000000
VERSION     DATISU010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276838"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VLR27_17985"
     CDS             <1..793
                     /locus_tag="VLR27_17985"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HSP05400.1"
                     /translation="FPTVMLILAALALILVIGTGSILLTLRIQRAEQRSMPTPAPAPD
                     PAATAGRRAPENVVERLAVALKRRRVSRALLSVLSIALLVGAAGMIGYPFYTNLYQDR
                     LQSQLDRQLASPDLRDAYVDRRVEEGDSLTRIQIPRIGVDTVVVEGTTASALRAGAGH
                     YPSTPLPCEVGNVAIAGHRTTYGKPFHALDVLEPGDRITLETPIGSCTYEVERSWITV
                     PTDVGVVDNTPGEARLTLTTCHPKGSARQRLILTATMVSAEAFGA"
     gene            786..1958
                     /locus_tag="VLR27_17990"
     CDS             786..1958
                     /locus_tag="VLR27_17990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05401.1"
                     /translation="MPERMRRLLVVLAATVLAAIALAPGAGAQSDEGPLVPSEDPEGR
                     WAGSAFTAPEADATVREPEVDVAGQFTYRKRTPGQRIVAATVEVQPDGFEPPATCETP
                     PSQPIPGNPPHPSDYDMDQPATLGFEVSDLRLPCNGRYLAHATAQLEDGTQWSMQVRF
                     GVAVAPPQVDEVTAELGDAERAATITFTPLELHERSPDEAGYLIERAGPDDGTFTEVT
                     TVTEGSEPRTVDDLGPAEPGRYVYRVRPLRNGVDGPVPSEAAGAPTAEIQLEGPPTTP
                     TTTPGSDAGADRQAPVRRNRSSSRVTSGSRRGTTTTRSVTPTTLDTGFEDTIDYGELP
                     DGSSEELAGDEPVAGQSIVQDEADDPVDLAVPAAGALVLLGWAGHIVYLNRLAKLL"
     gene            complement(1933..4338)
                     /locus_tag="VLR27_17995"
     CDS             complement(1933..4338)
                     /locus_tag="VLR27_17995"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSP05402.1"
                     /translation="MAGDRSDPCDLESLVRRRAAQLLDRDGPHLADASRLVHVERLAA
                     TEPRHAEPADPLPAPVAERLGVEQLYTHQAEALDLVRAGRSVVTATSTSSGKSLIYQA
                     AIGEAAIAPVRPGTALCLFPTKALAQDQLRSLAGLDLPGVQAATYDGDTGRAERGDAR
                     RNASVLLTNPEMLHCGILPHHAGWMRFLSRLRYVVVDELHLFRGIFGSHVAHVLRRLR
                     RVAAHYGADPVFICSSATIGEPAALASALTGVEVTAVTDDGSPSGERVVAIWNPPVLD
                     EESGTRASTHGEAAGLVAELVRADRRTIGFCPSRRSTELVADRVRRQVPASRRGRVAA
                     YRGGFLADERHQIEAALAEGELDAVVTTSALELGIDLEGLDACVLDGFPGTVASLWQR
                     VGRAGRRQQESLAVLVCGADQLDQWIAAHPEELFTRPPEPAVINPANPFVADPHLRCA
                     AFELPLTHDDARYWPGLLDEGVCRLAGSGELIVRPPGVGTAGGRGRRRRVSADSPVAV
                     WTGGGWPVDGIGLRSGSPGQVKIVQPDGTPVGTVDRGRAGEVVHEGAVYVHAGRHHRV
                     VELDLDRARAVVVPDDGSTWTTAITRMDLRILGEDRSREIGPVHLALGPICVTTRVTG
                     YERKDAASGERIERVGLDLPPSTLETRGVWQVVPEEVLAGLGLVPDAWPGVLHAAEHA
                     AIGVLPLFAICDRWDVGGVSTARHVDTGAPTIVIYDGYPGGAGIAELGFDVAERHLAA
                     TAQVIAACPCRAGCPSCVQSPKCGNGNEPLDKVGALRLLAALTGLDPVAAGAAPTAAW
                     RAG"
     gene            4450..4794
                     /locus_tag="VLR27_18000"
     CDS             4450..4794
                     /locus_tag="VLR27_18000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSP05403.1"
                     /translation="MELEIPDTDVDGWTVVAASGEIDVATAPALRDRLTELVEAGSTR
                     LVVDLEDVDFIDSTGLGVLVGGVRRARADDGDLRLVCTNARILKVFEATGLHEVFTIG
                     STVDDAVAAEAQ"
     gene            4804..>5065
                     /locus_tag="VLR27_18005"
     CDS             4804..>5065
                     /locus_tag="VLR27_18005"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP05404.1"
                     /translation="MAEIVLEIPPRLDYLAVVRLVVATAASLDPPLPDSRLDDLRLAV
                     TEACSNAIKAHRPEAADAPVVISCHLEEDLFRVEIRDRGPGFD"
BASE COUNT          685 a         1899 c         1855 g          626 t
ORIGIN      
        1 ttttccgacc gtcatgctca tcctcgccgc cctcgcgctc atcctggtga tcggcaccgg
       61 cagcatcctg ctgacgctgc ggatccagcg ggccgagcag cgctcgatgc ccacgccggc
      121 accagcgccg gacccggccg cgacggccgg tcgacgggcc ccggagaacg tggtcgagcg
      181 gttggccgtg gcgctcaagc gacgccgggt ctcccgggcg ctgctctccg tgctgtccat
      241 cgccctgctg gtcggcgccg ccgggatgat cggctacccc ttctacacga acctctacca
      301 ggaccggctg cagtcccagc tggaccgcca gctggccagc cccgacctcc gggacgccta
      361 cgtcgaccgc cgggtcgagg agggcgacag cctcacccgg atccagatcc cccgcatcgg
      421 cgtcgacacc gtcgtcgtcg agggcacgac ggccagcgcg ctgcgggccg gggccgggca
      481 ctacccgagc accccgttgc cgtgtgaggt cggcaacgtc gccatcgcag ggcaccgcac
      541 cacctacggc aagccgttcc acgccctcga cgtgctcgag cccggcgacc gcatcaccct
      601 cgagacgccg atcggctcgt gcacctacga ggtcgagcgg tcctggatca ccgtgccgac
      661 cgacgtcggc gtggtcgaca acaccccggg cgaggcgcgg ctcacgctga cgacgtgcca
      721 cccgaagggc tcggcccgcc agcgcctgat cctcaccgcc accatggtca gcgccgaggc
      781 cttcggtgcc tgagcgcatg cgccgcctgc tggtcgtgct cgccgcgacc gtgctcgccg
      841 cgatcgcgct cgcgcccggt gcgggcgcgc agtccgacga ggggccgctc gtgcccagcg
      901 aggaccccga gggccggtgg gccggatccg cgttcaccgc gccggaggca gatgccaccg
      961 tgcgcgagcc cgaggtcgac gtggccgggc agttcacgta ccgcaagcgc acccccggcc
     1021 agcgcatcgt cgccgccacc gtcgaggtgc agcccgacgg gttcgagccg ccggccacct
     1081 gcgagacccc gccctcccag ccgatccccg ggaacccgcc gcacccgtcc gactacgaca
     1141 tggaccagcc agcgaccctc ggcttcgagg tgtccgacct ccggctcccc tgcaacgggc
     1201 gctaccttgc ccacgccacg gcgcagctcg aggacggcac gcagtggtcg atgcaggtgc
     1261 gcttcggtgt cgccgtggcg ccaccccagg tcgacgaggt caccgccgag ctcggcgacg
     1321 ccgagcgcgc cgccaccatc accttcaccc cgctcgagct gcacgagcgg tcgcccgacg
     1381 aggccggcta cctcatcgag cgcgccggtc ccgacgacgg gaccttcacc gaggtgacga
     1441 ccgtgaccga gggctccgag ccccgcaccg tcgacgatct cgggccggcc gagcccggcc
     1501 ggtacgtgta ccgggtccgt ccgctccgca acggcgtcga tggtccggtg ccctccgagg
     1561 cggcgggtgc accgaccgcc gagatccagc tcgaggggcc accgaccacc ccgacgacga
     1621 cgccgggatc cgatgcgggg gccgaccgcc aggccccggt ccggcgcaac cggtcgtcca
     1681 gccgcgtgac gtcggggagc cgacgtggca ccaccacgac ccggagcgtc acgccgacga
     1741 cgctggacac cggcttcgag gacaccatcg actacgggga gctccccgac ggcagcagcg
     1801 aggagctcgc cggggacgaa ccggtcgccg ggcagtcgat cgtgcaggac gaggccgacg
     1861 accccgtcga cctcgccgtc ccggccgccg gcgcgctggt gctgctcggc tgggccgggc
     1921 acatcgtcta cctcaaccgg ctcgccaagc tgctgtaggg gccgcacccg ccgccaccgg
     1981 gtcgaggccg gtgagcgcgg cgagcaggcg cagcgcaccg accttgtcga ggggctcgtt
     2041 gccgttgccg cacttgggcg actgcacgca cgacgggcag ccggcgcggc aggggcacgc
     2101 ggcgatcacc tgggccgtgg cggcgaggtg gcgctcggcc acgtcgaagc cgagctcggc
     2161 gatgccggcg cccccggggt agccgtcgta gatcacgatg gtcggggcgc cggtgtcgac
     2221 gtggcgggcg gtggacacgc caccgacgtc ccagcggtcg cagatcgcga acagcggcag
     2281 caccccgatg gcggcgtgct cggcggcgtg gaggacaccc ggccaggcgt cggggacgag
     2341 gccgaggccg gcgaggacct cctcgggcac gacctgccag acgccccggg tctccagggt
     2401 gctgggcggc aggtcgaggc cgacccgctc gatgcgctcc cccgaggccg cgtccttgcg
     2461 ctcgtagccg gtgacgcgtg tggtgacgca gatcggaccg agggcgaggt gcacggggcc
     2521 gatctcccgg gagcggtcct cgccgaggat gcgcaggtcc atccgggtga tcgcggtggt
     2581 ccaggtgctg ccgtcgtcgg gcacgaccac ggcgcgggcc cgatcgaggt cgagctcgac
     2641 cacccggtgg tggcggccgg catggacgta gacggccccc tcgtggacca cctcgccggc
     2701 gcgaccgcgg tcgaccgtgc ccactggtgt gccgtcgggc tggacgatct tgacctggcc
     2761 tggcgagccc gagcgcaggc cgatcccgtc cacgggccat cccccgccgg tccacaccgc
     2821 caccggggaa tccgccgaca cccggcggcg ccgaccccgc ccgccggcgg tgcccacccc
     2881 gggcgggcgc acgatcagct cgccgctgcc ggccagccgg cacacgccct cgtcgagcag
     2941 gccgggccag tagcgggcgt cgtcgtgggt gaggggcagc tcgaaggccg cgcagcgcag
     3001 gtgggggtcg gccacgaagg ggttggccgg gttgatgacc gccggttcgg gtggccgggt
     3061 gaagagctcc tcgggatggg cggcgatcca ctggtcgagc tggtcggcac cgcagaccag
     3121 caccgccagg gactcctgct ggcggcggcc ggctcgtccg acccgctgcc agagtgacgc
     3181 gacggtgccg gggaagccgt cgagcacgca ggcgtcgagg ccctcgaggt cgatgccgag
     3241 ctcgagcgcc gaggtggtga ccacggcgtc gagctcgccc tcggccaggg cggcctcgat
     3301 ctggtggcgc tcatcggcga ggaagccacc ccggtaggcg gcaacccgcc ctcggcgtga
     3361 cgccggcacc tggcgccgga cccggtcggc caccagctcg gtggagcggc ggctcgggca
     3421 gaagccgatg gtgcgtcggt cggcccggac gagctccgca accagtccgg cggcctcacc
     3481 gtgggtggag gcgcgcgtgc cgctctcctc gtcgagcacc ggcgggttcc agatggccac
     3541 cacgcgctcg cccgacggcg acccgtcatc ggtgacggcg gtgacctcca cgccggtgag
     3601 cgccgacgcg agggcggctg gttcgccgat ggtggccgag gagcagatga acaccggatc
     3661 ggcgccgtag tgcgcggcga cgcgccgcag ccgtcgcagc acgtgggcga cgtgggagcc
     3721 gaagatcccc cggaacaggt gcagctcgtc gacgacgacg tagcgcagtc gggacaggaa
     3781 ccgcatccac ccggcgtggt gcgggaggat cccgcagtgc agcatctcgg ggttggtgag
     3841 cagcacgctg gcgttgcggc gggcgtcgcc gcgctcggcc cggccggtgt cgccgtcgta
     3901 ggtggcggcc tgcacgccgg gcaggtcgag gccggcgagc gagcggagct ggtcctgggc
     3961 cagggccttg gtggggaaca ggcacagggc ggtgccggga cggaccgggg cgatcgcggc
     4021 ctcgccgatc gcggcctggt agatcaacga cttgcccgac gaggtgctgg tcgccgtgac
     4081 caccgagcga ccggcccgga ccaggtccag cgcctcggcc tggtgggtgt agagctgctc
     4141 gaccccgagg cgctcggcga ccggcgccgg caacgggtcg gccggctcgg cgtgtcgggg
     4201 ctcggtggcg gcgaggcgct cgacgtggac gagccggctt gcatcggcca ggtgggggcc
     4261 gtcccggtcg aggagctggg cggcgcgccg gcgcacgagc gactcgaggt cgcagggatc
     4321 ggagcgatcg ccggccacgt cggcgatcgt acgcccccac cccgccatca cgacaggcct
     4381 gtagttccac gcctgtgctt cctccggcct gcagtcgaag aggcctcgac cggtagcgtg
     4441 gtcgactgca tggagctgga gatccctgac accgacgtcg acggctggac cgtcgtggcc
     4501 gcctccggcg agatcgacgt cgccaccgcg cccgcgctgc gcgatcggct caccgagctc
     4561 gtcgaagccg gctcgacccg gctcgtcgtc gacctggagg acgtcgactt catcgactcc
     4621 accggcctcg gcgtgctggt cggcggggtc cgccgggcac gggccgatga cggcgacctg
     4681 cggctggtct gcaccaacgc ccggatcctg aaggtcttcg aggccaccgg tctgcacgag
     4741 gtcttcacga tcggttcgac ggtggacgac gccgtcgcgg ccgaggccca gtaggcgacc
     4801 accgtggccg agatcgtcct cgagatcccc cctcggctgg actacctcgc cgtcgtcagg
     4861 ctcgtcgtcg ccaccgccgc gtcgctcgac cccccgctcc ccgattcccg cctcgacgac
     4921 ctccgcctcg cggtcaccga ggcgtgcagc aacgccatca aggcgcaccg gcccgaggcg
     4981 gccgacgctc ccgtggtgat ctcctgccac ctggaggagg acctgttccg ggtcgagatc
     5041 cgcgaccgcg gtcccggctt cgacc
//
LOCUS       DATISU010000451         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_742743, whole genome shotgun sequence.
ACCESSION   DATISU010000451 DATISU010000000
VERSION     DATISU010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742743"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VLR27_18010"
     CDS             <1..675
                     /locus_tag="VLR27_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSP05405.1"
                     /translation="RDFAQGDMIARQMVNFVYLVGDRETGEAVAIDPAYDVQGLVDVL
                     AEDGMRLTGALATHYHPDHVGGDMMGHSIQGVADLLAIAPVPIHIQEEEKPWVQRVTG
                     ASDTDLVTHVSGDTVMVGEIPIELIHTPGHTPGSQCFLVDNRLVAGDTLFLEGCGRTD
                     LPGGDAGQLYESLTQKLAKVPDDATLFPGHLYSPEPSAPMDEVRRFNMVFQPTDKDTF
                     LRMFGR"
     gene            complement(727..1509)
                     /locus_tag="VLR27_18015"
     CDS             complement(727..1509)
                     /locus_tag="VLR27_18015"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSP05406.1"
                     /translation="MHSQDLHVETYGTPSGRPLLAIHGITGFGARFERLAGLVDRPRW
                     ICPDLRGHGRSPTEPPWRTEDHVADLLRVLDAFDLEEVDVVGHSFGGHLALHLLALAP
                     ERVGRMVLLDPATLIDPAGAEAGALDYARDPGWATEDEARAEIATWVPNEGSQPDLAP
                     EIERNLLQDDDGRWRMGFSPVVVVAAYSEMCRPLPALPARDVLLVEADPGQTTVNPTL
                     RDALERAFGARLRREVVEGATHVLFRTELEATAEVMRPFLTT"
     gene            complement(1568..2167)
                     /locus_tag="VLR27_18020"
     CDS             complement(1568..2167)
                     /locus_tag="VLR27_18020"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HSP05407.1"
                     /translation="MELSSPAGAFASAHARRWLLRAAVVIGIVGLVAAVMTSGVLDRF
                     TDQDELQATIDRAGVWGPLLFVGLMVLLVPLNVPGLLFVVPATTLFGTVGGVLLSLIG
                     GFLASTIGIVGARRLGRDAFIARLPPRLRRLEARLCERGFWAVVALRSVTFLLQPVDW
                     LCGLTRMPMRTVLSATFVGLVPPTLVIALTGGGVLDLVL"
     gene            2229..>3048
                     /locus_tag="VLR27_18025"
     CDS             2229..>3048
                     /locus_tag="VLR27_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HSP05408.1"
                     /translation="MPDEILDVDLLAFERGDAAQRRAVVDGVMRSLATGFVYTATDLS
                     ETMLDDAYGMLRRFFELDQERKDAWIAPGTHGQAGYTGLLVETAATSEVPDWKEMLNW
                     GPTVPEGHPMRRKYPHRFHDPVLPEADVPGITAVLTEFHERVFDTQRRFLRIIALGLG
                     AHEALFEDLLKDGPVLNRAIRYPPMSSAPEAPHEWAGAHADINLTTVLPRATTAGLQV
                     RTKAATEDAAAVWVDAAPPEGHVILNTGIMLERMSNGIIPSGWHRVVADPDHDGE"
BASE COUNT          472 a         1098 c         1119 g          359 t
ORIGIN      
        1 cgcgacttcg cccaggggga catgatcgcc cgccagatgg tgaacttcgt ctacctggtg
       61 ggcgaccggg agaccggcga ggcggtggcc atcgacccgg cctacgacgt gcagggcctc
      121 gtcgacgtcc tcgccgagga cggcatgcgc ctcaccggtg cgctcgccac ccactaccac
      181 ccggaccacg tgggcgggga catgatgggc cactcgatcc agggcgtcgc cgacctgctc
      241 gccatcgccc cggtgccgat ccacatccag gaggaggaga agccctgggt ccagcgggtc
      301 accggcgcca gcgacaccga cctggtcacc cacgtctccg gcgacacggt gatggtgggc
      361 gagatcccga tcgagctgat ccacacgccc gggcacacgc cgggcagcca gtgcttcctg
      421 gtcgacaacc gcctcgtcgc cggcgacacg ctgttcctcg aggggtgcgg gcgcaccgac
      481 ctgccggggg gcgacgccgg ccagctctac gagtcgctca cccagaagct ggccaaggtg
      541 cccgacgacg ccacgctctt ccccggccac ctgtactccc cggagccctc cgccccgatg
      601 gacgaggtcc gccgcttcaa catggtgttc cagccgacgg acaaggacac gttcctgcgc
      661 atgttcggcc gctgaggcgc aacaggagca ctgccagaag cgaaaggggg cccgccccgg
      721 cgccggtcac gtggtgagga aggggcgcat gacctcggcg gtcgcctcga gctcggtgcg
      781 gaacagcacg tgggtggcgc cctcgacgac ctcccggcgg aggcgggccc cgaacgcccg
      841 ctccagcgcg tcgcgcaggg tcgggttcac ggtggtctgg cccggatcgg cctcgacgag
      901 cagcacgtcg cgggcgggca gcgcggggag cgggcggcac atctcgctgt aggcggcaac
      961 gaccaccacg ggcgagaagc ccatgcgcca gcggccgtcg tcgtcctgga gcaggttgcg
     1021 ctcgatctcg ggcgccaggt cgggctggga gccctcgttg gggacccagg tggcgatctc
     1081 ggcccgggcc tcgtcctcgg tggcccagcc cgggtcccgg gcgtagtcca gggcgccggc
     1141 ctcggcgccg gccgggtcga tcagcgtcgc cgggtcgagc agcaccatgc gccccacccg
     1201 ctccggggcg agggcgagga ggtggagggc gaggtggccg ccgaaggagt ggccgaccac
     1261 gtcgacctct tcgaggtcga aggcatcgag caccctcagc aggtcggcga cgtggtcctc
     1321 cgtgcgccac ggcggctccg tcggcgagcg gccgtgcccc cgcaggtccg ggcagatcca
     1381 ccggggtcgg tcgaccaggc cggccaaccg ctcgaagcgg gccccgaagc cggtgatgcc
     1441 gtggatggcg aggagcggac gaccggaggg ggtgccgtag gtctcgacgt ggaggtcctg
     1501 ggagtgcacg gcctccggtc taccgccgcg accgcgacac cacgactgcc agttcgagag
     1561 gggcgggtca gaggacgagg tcgagcacac cgccgccggt gagcgcgatg accagcgtcg
     1621 ggggcacgag gccgacgaag gtggcgctca gcacggtccg catcggcatg cgggtgagcc
     1681 cgcacagcca gtcgaccggc tggagcagga aggtgacgct gcggagggcg acgacggccc
     1741 agaagccccg ctcgcacagg cgggcctcga gccggcgcag ccgcggtggc aggcgggcga
     1801 tgaaggcgtc gcggccgagg cggcgggcac cgacgatgcc gatggtgctg gcgaggaagc
     1861 cgccgatcag ggacagcagc acgccgccca ccgtgccgaa cagcgtggtg gccggcacga
     1921 cgaagagcag gcccggcacg ttcagcggca cgagcagcac catgaggccg acgaagagca
     1981 gcggtcccca caccccggcc cggtcgatgg tcgcctggag ctcgtcctgg tcggtgaagc
     2041 ggtcgaggac cccggaggtc atcaccgcgg ccacgagacc gacgatgccg atcaccaccg
     2101 cggcgcgcag gagccagcgc ctggcgtgcg cggacgcgaa ggctccggcg ggggaggaca
     2161 gctccatgca ccggtgttcg ccgccacgtc cgggtgttcc tgccggtaac accagtacgg
     2221 tcggggacgt gcctgacgag atcctcgacg tcgacctgct ggccttcgag cggggcgacg
     2281 ccgcccagcg ccgggcggtg gtcgacggcg tcatgcggag cctggcgacc ggcttcgtgt
     2341 acaccgccac cgacctgtcc gagaccatgc tcgacgacgc ctacgggatg ctgcggcggt
     2401 tcttcgagct ggaccaggag cgcaaggacg cctggatcgc cccgggcacg cacggccagg
     2461 ccggctacac cggcctgctg gtcgagaccg ccgccacgtc cgaggtgccg gactggaagg
     2521 agatgctcaa ctggggcccg acggtccccg aggggcaccc gatgcggcgc aagtacccgc
     2581 accggttcca cgatccggtg ctgcccgagg ccgacgtgcc cgggatcacc gccgtgctca
     2641 ccgagttcca cgagcgcgtc ttcgacaccc agcgccggtt cctgcgcatc atcgccctcg
     2701 gcctcggcgc ccacgaggcg ctgttcgagg acctgctgaa ggacggcccc gtgctcaacc
     2761 gggccatccg ctacccgccg atgtcgtccg cgcccgaggc accgcacgag tgggcgggtg
     2821 cccacgccga catcaacctc accaccgtcc tgccccgggc cacgaccgcg ggcctgcagg
     2881 tgcgcacgaa ggcggccacc gaggacgccg ccgccgtctg ggtcgacgcc gccccgccgg
     2941 agggccacgt gatcctcaac accgggatca tgctcgagcg gatgtcgaac gggatcatcc
     3001 cgtcgggctg gcaccgggtg gtggccgacc ccgaccacga cggcgagc
//
LOCUS       DATISU010000452         7120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_417335, whole genome shotgun sequence.
ACCESSION   DATISU010000452 DATISU010000000
VERSION     DATISU010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417335"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VLR27_18030"
     CDS             complement(<1..279)
                     /locus_tag="VLR27_18030"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HSP05409.1"
                     /translation="MTAELHDIATARLRTTGQRYTTRRRALVEVLDQADGPLTIPPIL
                     ALARSLAQSSAYRNRAVLEQAEVVHRLVTNDDVARYELAEDLTEHHHHL"
     gene            complement(276..1037)
                     /gene="aztA"
                     /locus_tag="VLR27_18035"
     CDS             complement(276..1037)
                     /gene="aztA"
                     /locus_tag="VLR27_18035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein AztA"
                     /protein_id="HSP05410.1"
                     /translation="MPPVPHLRIRDLHVQHGARVALTGVSLDVEAGTTVAVIGANGSG
                     KSTLLRSIAGLQAPSGGSIDTGGLDVALVLQTTTVDGSLPITVRETVRLARAAERGPW
                     RRLRGEDHAIVDQAMDRLEVADLADRALHELSGGQRQRVLVAQGLAQRAPILLLDEPV
                     TGLDMVSKEAILRVIAEEAADGRIVCTSTHDLDEARRCDRAVLLATHLVAEGPPDAVL
                     TEANLQEAFEGRVLRLPDGQLLVDDVHLHHHGSEW"
     gene            1153..2166
                     /locus_tag="VLR27_18040"
     CDS             1153..2166
                     /locus_tag="VLR27_18040"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HSP05411.1"
                     /translation="MPRTRALALPLAAVLAGSLLAACGSDDADAGDEAGRPTVAVTTT
                     VLGDVVSELVGDLAEVVTIMPPGASPHDFQASAQQAAELAGADVIVVNGGGFEEGLLS
                     VVEGAESDGVPVLSALELLGGEDDHADEDDHADEEDHADEEDHADEEEHADEDEHAHD
                     DDAHFFTDPLAVVEVVEALAVSLPEQVADLDAGQLRQQADALVGDLEALHEELVATLA
                     GVPAERRVLVTDHEVFGSFAARYDFEILATVIPAGSTSDGVSGGALAQLAEVLRTAGV
                     TAVFTEQTASADLADTLASEVGDVEVVALNAETLGPDGSEADTYAGMMRLNAQRIADA
                     LGG"
     gene            2179..3423
                     /gene="aztB"
                     /locus_tag="VLR27_18045"
     CDS             2179..3423
                     /gene="aztB"
                     /locus_tag="VLR27_18045"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease AztB"
                     /protein_id="HSP05412.1"
                     /translation="MLDLVTEAFEPAFMQRALLGSLVAVVATSLVGTWVVLRGLAFLG
                     DALAHGVIPGIALAVLWGFSPVIGALGAALVMSGAVTLVATRSAVREDTGIGLLFVGM
                     LSLGVIIVSRSETFTTDVTTLLFGDVLGVTAADIRGQAVAAAVVLAVIVLGHRPFLAL
                     TFNRDKAATLGMHPALAQAALLAVLAVSIVASFQAIGTLLVFGLLVGPPATASLLVRR
                     VPLMMVTSVAAGAVAVVIGLLVSFHHGTAGGATIAAVSVGLFFLVLVGVELRRWQQVR
                     APAVGSAVGGLDQVLGLAQLQDAPGRRGRRTDLQVSARSPEPGGVRADHLDAVAAQGV
                     DPARIDDDPVAAVGSQLAEPAVQHRGRVGAQRPVQHAHRDLSLEAQGDLEDVGGATTL
                     DGGLEQPRRHRAASRRSLQPAP"
     gene            complement(3381..3749)
                     /locus_tag="VLR27_18050"
     CDS             complement(3381..3749)
                     /locus_tag="VLR27_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05413.1"
                     /translation="MSVVRVEHVEGARVIHVEGCATDDDLRQALTSLRDHAGPTVLDL
                     AELTLVGPGVAELVAGLVETCGAVCVTARRHTARVILQRSGIPDLCVVFTSVGDALQA
                     LRLAEAGYGAGWSERLEAAR"
     gene            complement(3940..4296)
                     /locus_tag="VLR27_18055"
     CDS             complement(3940..4296)
                     /locus_tag="VLR27_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05414.1"
                     /translation="MQHADTADAVLTPRPVAEAPVSQDQANRTFSTSVLVSAVRCTLA
                     YVVFPWLLPAFGLASGVGPALGLVIGPIAVAFNVASIRRFHASDHRWKWQITALNSAV
                     IVLLAVLFVLDLRALG"
     gene            complement(4353..5810)
                     /locus_tag="VLR27_18060"
     CDS             complement(4353..5810)
                     /locus_tag="VLR27_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HSP05415.1"
                     /translation="MADIKGFLKYERELPSRRPVPVRLRDWKEVYEDFPEDHLQKQAA
                     RCMDCGIPFCNNGCPLGNLIPDWNDLVYRDRWRDAIERLHATNNFPEFTGRLCPAPCE
                     GACVLGINQDPVTIKQVEVEIIDRAFEEGWVTPVLPSTKTGKKVAVVGSGPAGLAAAQ
                     QLTRAGHDVVVFERADRIGGLLRYGIPEFKMEKRHIDRRLDQMAAEGTEFRTNADVGV
                     DVSVDDLRRDFDAIVLAGGATQWRDLPIPGRDGEGVHQAMEYLPWANKAQHGDIAVDE
                     VPINAAGKNVVIIGGGDTGADCLGTAHRQGAASIHQFEIMPRPPEERPDANPWPTYPM
                     VYKVTSAHEEGGDRVYSVNTEEFVRDGDGNLVGLKAHEVEMVDGRFQKVEGSDFELEC
                     ELVLLAMGFTGPESGGLLDQLGVERDERSNVARDAVYKTNLDDVWVCGDMGRGQSLIV
                     WAIAEGRACAAAVDEALMGETALPVPVAPTDRPLV"
     gene            complement(5816..>7120)
                     /locus_tag="VLR27_18065"
     CDS             complement(5816..>7120)
                     /locus_tag="VLR27_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HSP05416.1"
                     /translation="RDRIVVQCDGQLKTGRDVVIAALLGAEEFGFATAPLVVSGCIMM
                     RVCHLDTCPVGVATQNPELRKRFNGKAEFVVNFMEFIAEEVREYLAQLGFRTMEEAIG
                     QVDALDTTKAVQHWKADGLDLSPILHKPESPWEQDLHCSKTQDHGLDAALDNELIERS
                     RPAIEDGEPVEIELPIRNLNRTVGTMLGNRISVRHGEAGLPDDTIQIRFTGSAGQSLG
                     AFLPKGVTIRLNGDTNDYLGKGLSGGRLVVRPPEESHPKLVAEDNVIAGNVILYGATA
                     GEVYIRGVVGERFCVRNSGATAVVEGVGDHACEYMTGGRAVVLGETGRNFGAGMSGGI
                     AYVWDPEDLFHRNLNNEMVDLDPLDETDAAWLRDTIRTHLAETNSAVAQRILDRWHTN
                     VRHFKKVMPEDFKRVLEAAEKARIEGVDVDEAIMASAALEKE"
BASE COUNT          914 a         2510 c         2609 g         1087 t
ORIGIN      
        1 gaggtggtgg tggtgctcgg tgaggtcctc ggccagctcg tatcgggcga cgtcgtcgtt
       61 cgtgacgagg cggtggacga cctcggcctg ctcgagcacg gcccggttgc ggtacgcgct
      121 cgactgggcg agcgagcgag cgagcgcgag gatcgggggg atcgtgagcg gcccgtcggc
      181 ctggtcgagc acctccacca aggcgcgccg ccgggtggtg tagcgctggc cggtcgttcg
      241 cagccgggcg gtggcgatgt cgtgcagctc ggcggtcacc actcgctccc gtggtggtgg
      301 aggtggacgt cgtcgaccag cagctgcccg tccggcaggc gcaggacccg gccctcgaag
      361 gcctcctgca ggttggcttc ggtgaggacc gcgtcgggcg gaccttctgc gaccaggtgc
      421 gtggcgagca ggacggctcg gtcgcagcgg cgggcctcgt cgaggtcgtg ggtggaggtg
      481 cacacgatgc gtccgtcggc ggcctcctcg gcgatgacgc gcaggatggc ctccttcgac
      541 accatgtcga gcccggtcac cggctcgtcc agcaggagga tcggcgcgcg ctgggcgagg
      601 ccctgggcca ccagcacgcg ctggcgctgg ccgccggaga gctcgtgcag ggcccggtcg
      661 gccaggtcgg cgacctccag gcgatccatg gcctggtcca cgatggcgtg gtcttccccc
      721 cgcagccgcc gccacggacc gcgctcggcg gcccgtgcga gccggacggt ctcccgcacc
      781 gtgatgggca gggacccgtc cacggtcgtc gtctgcagca ccagggccac gtcgaggccg
      841 ccggtgtcga tcgagccgcc cgacggtgcc tggagtccgg cgatgctgcg cagcaacgtc
      901 gacttccccg acccgttggc gccgatgacg gcgacggtcg tgcccgcctc cacatcgagg
      961 ctcacgccgg tcagcgccac gcgggccccg tgctgcacgt gcaggtcacg gatccggagg
     1021 tgggggacgg ggggcacgag gtgacggtac cggaaacggg tccggttctc atttgcagat
     1081 ccgggcgatc gttcgtacgc tcgtctcgat accgagaact gttctcattc accgctcgtt
     1141 cctgggaatc cgatgcctcg cacccgtgcc ctcgccctac ccctcgccgc cgtcctcgcc
     1201 gggtcgctcc tggctgcatg cggctccgac gacgccgacg ccggagacga ggccggtcga
     1261 ccgaccgtgg ccgtcaccac cacggtgctc ggcgacgtgg tctcggagct ggtcggcgac
     1321 cttgccgagg tcgtgacgat catgccgccg ggtgccagtc cccacgactt ccaggcgtcg
     1381 gcgcagcagg cggccgagct cgccggcgcc gacgtcatcg tcgtcaacgg cggcgggttc
     1441 gaggaaggcc tgctgtcggt cgtcgaaggc gcggagtctg acggcgtccc ggtgctgtcg
     1501 gccctggagc tgctgggcgg cgaggacgat cacgccgacg aggacgatca cgccgacgag
     1561 gaggaccacg ccgacgagga ggaccacgcc gacgaagagg agcacgccga cgaggacgag
     1621 cacgcccacg acgacgacgc ccacttcttc accgacccgc tcgccgtcgt cgaggtggtc
     1681 gaggcgctgg ccgtgtccct gcccgagcag gtcgcggatc tcgatgccgg ccagctgcgc
     1741 cagcaggccg atgcgctcgt cggtgacctc gaggcgctgc acgaggagct ggtcgccacc
     1801 ctcgccggcg tgccggcgga acgccgggtg ctggtcaccg accacgaggt cttcggctcc
     1861 ttcgccgccc ggtacgactt cgagatcctg gccacggtga tccccgccgg ctcgacgtcc
     1921 gacggggtga gcggcggcgc gctggcgcag ctcgccgagg tgctgcggac cgccggtgtc
     1981 acagcggtct tcaccgagca gacggcgtcg gccgacctcg ccgacaccct ggcctccgag
     2041 gtcggcgacg tcgaggtcgt cgccctcaac gccgagacgc tcggtcccga cggctccgag
     2101 gccgacacct acgctgggat gatgcggctg aacgcgcagc gcatcgccga cgcgctcggc
     2161 ggctgaggga gcggcgccgt gctcgacctg gtgaccgagg cgttcgagcc ggcgttcatg
     2221 cagcgcgccc tgctcggcag cctcgtcgcc gtggtcgcca cgtcgctggt cggcacctgg
     2281 gtggtgctgc gcggcctcgc cttcctcggc gacgccctgg cccacggcgt gatccccggc
     2341 atcgccctgg ccgtgctgtg ggggttcagc ccggtcatcg gggcgctcgg cgccgcgctg
     2401 gtgatgagcg gcgcggtgac cctcgtggcg acccggagcg ccgtgcgcga ggacaccggc
     2461 atcggcctgc tcttcgtcgg aatgctctcg ctcggcgtga tcatcgtgtc ccgctcggag
     2521 accttcacca ccgatgtgac cacgctgctg ttcggcgacg tcctcggcgt gaccgccgcc
     2581 gacatccggg gccaggcggt ggccgccgcc gtggtcctcg ccgtcatcgt gctcgggcac
     2641 cgccccttcc tggccctgac cttcaaccgg gacaaggccg cgacgctcgg catgcacccg
     2701 gcgctggcgc aggccgccct cctcgccgtg ctcgccgtga gcatcgtcgc cagcttccag
     2761 gccatcggca cgctgctcgt gttcggcctc ctggtcggac caccggcgac ggcgtcgctg
     2821 ctggtgcggc gggtgccgct catgatggtg acctcggtgg ccgccggcgc cgtggcggtc
     2881 gtgatcggcc tgctcgtcag cttccaccac ggcaccgccg gcggtgccac catcgccgcc
     2941 gtgtccgtcg gcctgttctt cctggtcctg gtcggggtgg agctgcgccg ctggcagcag
     3001 gtccgcgccc ccgccgtcgg atcagccgtc ggcggcctcg accaggtgct cggcctggca
     3061 cagctgcagg acgcgccggg ccggcgcgga cggcgaacgg acctgcaggt gtccgcccgc
     3121 agcccggagc cgggcggcgt gcgcgcggat cacctcgacg ccgtcgctgc tcagggagtc
     3181 gaccccgccc gcatcgacga cgacccggtc gcggccgttg gctcgcagct ggccgagcca
     3241 gccgtccagc accggggccg tgtaggcgca cagcggcccg tccagcacgc acaccgtgac
     3301 ctctccctcg aagcgcaggg tgatctcgag gacgtcggtg gcgccaccac cctggacggt
     3361 gggctcgaac aaccacgtcg tcatcgcgcc gcctccaggc gctcgctcca gcccgcgccg
     3421 tagccggcct cggcgaggcg gagggcctgc agggcgtcgc cgacgctggt gaacaccacg
     3481 cacaggtcgg ggatgccgct gcgctggagg atgacgcggg cggtgtggcg gcgggcggtg
     3541 acgcacaccg cgccgcacgt ctcgaccagg ccggcgacca gctcggccac gcccgggccc
     3601 acgagcgtca gctcggccag gtccaggacg gtcggcccgg cgtggtcgcg cagcgacgtc
     3661 agggcctggc ggaggtcgtc gtcggtggcg cagccctcca cgtggatgac gcgtgcgccc
     3721 tcgacgtgct ccacgcgcac gacgctcatc gcgtcgctcc cggtccggtg atcggtgacg
     3781 gtgggtcgcc cggatcgatg tgtgccatcg gttgtccccc tttggatgcg ccgcccgaca
     3841 aggagcgccc gcgggggtca cggcggacga ggatcgacca tacccaggga ttcgcgttgt
     3901 caccaccgtc acaccggatt ctccggcgct ggcgaggcgt cagccgaggg cgcgcaggtc
     3961 caggacgaag aggacggcca gcaggacgat caccgcgctg ttgagggcgg tgatctgcca
     4021 cttccagcgg tggtcgctgg catggaaccg tcggatgctg gccacgttga acgcgaccgc
     4081 gatcggtccg atcacgagcc cgagcgccgg gccgacgccg ctggccaggc cgaaggccgg
     4141 gaggagccac gggaacacga cgtaggcgag cgtgcagcgc accgcggaga cgagcaccga
     4201 ggtgctgaac gtgcggttcg cctggtcctg gctgacgggg gcttccgcca ccggccgggg
     4261 ggtcaggact gcgtcggccg tgtcggcgtg ctgcacccgc ccagtctcgt cgccgagtac
     4321 cgggcggggc aaagcgccgg cgggaccgct cgtcacacca acgggcggtc ggtgggggcc
     4381 accgggacgg gcagggcggt ctcgcccatg agcgcctcgt cgacggcagc cgcgcaggcc
     4441 cgaccctcgg cgatggccca cacgatgagg ctctggcccc ggcccatgtc gccgcacacc
     4501 cacacgtcgt ccaggttcgt cttgtagacg gcgtcgcggg cgacgttcga gcgctcgtcc
     4561 cgctcgacgc cgagctgatc gagcagcccg cccgactccg ggccggtgaa gcccatggcg
     4621 agcagcacga gctcgcactc gagctcgaag tcgctgccct ccaccttctg gaagcggccg
     4681 tcgaccatct ccacctcgtg ggccttcagg ccgaccaggt tgccgtcgcc gtcgcgcacg
     4741 aactcctcgg tgttcacgct gtagacgcgg tcaccgccct cctcgtgggc ggaggtgacc
     4801 ttgtagacca tcggataggt gggccacggg ttggcgtcgg ggcgctcctc cggcgggcgg
     4861 ggcatgatct cgaactggtg gatgctggcc gcgccctggc ggtgggcggt gcccaggcag
     4921 tcggcgccgg tgtcgccgcc gccgatgatc accacgttct tgcccgcagc gttgatgggc
     4981 acctcgtcca cggcgatgtc gccgtgctgg gccttgttcg cccacggcag gtactccatc
     5041 gcctggtgca cgccctcgcc gtcgcgcccg gggatcggca ggtcccgcca ctgggtggcg
     5101 ccgccggcga gcacgatcgc gtcgaagtcg cggcgcaggt cgtcgaccga cacgtcgacg
     5161 cccacgtcgg cgttggtgcg gaactcggtg ccctcggccg ccatctggtc gaggcgccgg
     5221 tcgatgtggc gcttctccat cttgaactcg gggatgccgt agcgcaggag gccgccgatg
     5281 cggtcggcgc gctcgaacac gaccacgtcg tggccggccc gggtcagctg ctgggcggcg
     5341 gcgaggccgg cgggaccgga gccgacgacc gcgaccttct tgcccgtctt ggtcgagggc
     5401 aggacggggg tgacccagcc ctcctcgaac gcccggtcga tgatctcgac ctcgacctgc
     5461 ttgatggtga ccgggtcctg gttgatgccg agcacgcagg cgccctcgca cggcgccggg
     5521 cacaggcggc cggtgaactc ggggaagttg ttggtggcgt gcaggcgctc gatggcgtcg
     5581 cgccagcggt cccggtagac gaggtcgttc cagtcgggga tgaggttccc gagcgggcag
     5641 ccgttgttgc agaacgggat gccgcagtcc atgcaccggg ccgcctgctt ctgcaggtgg
     5701 tcctcgggga agtcctcgta gacctccttc cagtcgcgca accgcacggg caccggacgc
     5761 cgggagggaa gctcgcgctc gtacttgagg aagcccttga tgtcagccat gtcgtctact
     5821 ccttctcgag cgccgcggac gccatgatcg cttcgtccac gtcgacgcct tcgatgcggg
     5881 ccttctccgc tgcctcgagg acccgcttga agtcctcggg catgaccttc ttgaagtggc
     5941 gcacgttggt gtgccaccgg tcgaggatcc gctgggccac ggccgagttg gtctcggcca
     6001 ggtgggtgcg gatcgtgtcg cggagccagg ccgcgtcggt ctcgtcgagc gggtcgaggt
     6061 cgaccatctc gttgttcagg ttgcggtgga acaggtcctc ggggtcccac acgtaggcga
     6121 tgccgcccga catgccggcg ccgaagttgc ggcccgtctc gccgagcacc acggcccggc
     6181 cacccgtcat gtactcgcag gcgtggtcgc ccacaccctc gaccaccgcg gtggcgccgg
     6241 agttgcgcac gcagaagcgc tcgcccacga cgccgcggat gtacacctcg ccggcggtgg
     6301 ccccgtagag gatcacgttg ccggcgatca cgttgtcctc cgcgaccagc ttggggtggc
     6361 tctcctccgg cgggcgcacc acgaggcggc caccggacag gcccttgccg aggtagtcgt
     6421 tggtgtcgcc gttgaggcgg atcgtgacgc ccttgggcag gaacgccccg aggctctggc
     6481 cggcggaccc ggtgaagcgg atctggatgg tgtcgtccgg caggccggcc tcgccgtggc
     6541 ggaccgagat ccggttgccg agcatggtgc cgacggtgcg gttgaggttc cggatcggca
     6601 gctcgatctc caccggctcg ccgtcctcga tggcggggcg ggagcgctcg atcagctcgt
     6661 tgtcgagggc ggcgtcgagg ccgtggtcct gggtcttgga gcagtgcagg tcctgctccc
     6721 acggtgactc cggcttgtgg aggatggggg acaggtcgag gccgtcggcc ttccagtgct
     6781 ggacggcctt ggtggtgtcg agcgcatcga cctggccgat ggcctcctcc atggtgcgga
     6841 agcccagctg ggccaggtac tcgcgcacct cctcggcgat gaactccatg aagttcacga
     6901 cgaactcggc cttgccgttg aaccgcttgc gcagctcggg gttctgcgtg gccacgccca
     6961 ccgggcacgt gtcgaggtgg cagacccgca tcatgatgca gcccgacacc acgagcggtg
     7021 cggtggcgaa gccgaactcc tcggcgccga gcagggcggc gatgacgacg tcgcggccgg
     7081 tcttgagctg gccgtcgcac tgcacgacga tgcggtcgcg
//
LOCUS       DATISU010000453         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_464249, whole genome shotgun sequence.
ACCESSION   DATISU010000453 DATISU010000000
VERSION     DATISU010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464249"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /gene="dnaB"
                     /locus_tag="VLR27_18070"
     CDS             complement(<1..1196)
                     /gene="dnaB"
                     /locus_tag="VLR27_18070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HSP05417.1"
                     /translation="MPPSDLVAEQNLLGAMMLSKDAISATSTMLRPADFSKPAHAHIF
                     DAILSLDSAGEPADPVTVADELARAGVLEAIGGRSLLAEIVSTAPAVSNAARYARIIS
                     DHAVLRKLIGVGGEIAELGYDVPDDVEKAVDTAESLVYELGQRRVSDSMVPIESLLGD
                     TLDRLEALVERGDAITGLPTGFVDLDRLLSGLQPNALIIVGARPAMGKTSFALNLTAN
                     AAFQSRRPVIYFSLEMGNLELTQRLLCAEAQIDSTKVRDGRLQDAEWDRIALGVSRLA
                     EARLYLDDNPNISVMEIRAKARRLKSQVGDLGLVVIDYLQLMQGRGRAESRQIEVSEM
                     SRGLKILARELECPVIALSQLSRTLESRTDKRPMLSDLRESGSLEQDADVVMFLYRDE
                     VYNPDSP"
     gene            complement(1488..1934)
                     /gene="rplI"
                     /locus_tag="VLR27_18075"
     CDS             complement(1488..1934)
                     /gene="rplI"
                     /locus_tag="VLR27_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HSP05418.1"
                     /translation="MRVILRTDVDMVGKRGDLVDVSDGHARNHLIPKGLAMRATADAE
                     LQAEQMRKAREVRDTKAIEAAQEVATALVPQTIHITAKAAENGHLFGAVHESEIATAV
                     KAQTEIELDKKIIIIDEPIKTVGTHYVMAKLHQEVQFPITIEVEAL"
     gene            complement(2105..2294)
                     /gene="rpsR"
                     /locus_tag="VLR27_18080"
                     /pseudo
     CDS             complement(2105..2294)
                     /gene="rpsR"
                     /locus_tag="VLR27_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004005948.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S18"
BASE COUNT          303 a          723 c          880 g          388 t
ORIGIN      
        1 ggtgagtccg ggttgtagac ctcgtcgcgg tacaggaaca tcacgacgtc ggcgtcctgc
       61 tcgagcgagc ccgactcgcg caggtccgac agcatcgggc gcttgtcggt gcgggactcg
      121 agcgtgcggg acagctggga cagggcgatc accgggcact cgagctcgcg ggcgaggatc
      181 ttgaggccac gggacatctc cgagacctcg atctggcggg actcggcccg accccggccc
      241 tgcatgagct gcaggtagtc gatgacgacg aggccgaggt cgcccacctg ggacttgagg
      301 cggcgggcct tggcccggat ctccatgacc gagatgttgg ggttgtcgtc caggtagagg
      361 cgggcctcgg ccaggcggga cacgccgagg gcgatgcggt cccactcggc gtcctggagc
      421 cggccgtcgc gcaccttggt ggagtcgatc tgggcctcgg cgcagagcag gcgctgggtg
      481 agctcgaggt tgcccatctc caggctgaag tagatgaccg gccggcggga ctggaacgcg
      541 gcgttggccg tgaggttcag ggcgaagctc gtcttgccca tggcgggacg ggccccgacg
      601 atgatgaggg cgttgggctg caggcccgac agcagccggt cgaggtcgac gaagccggtg
      661 ggcaggccgg tgatggcatc gccccgctcg accagggcct cgaggcggtc gagggtgtcg
      721 ccgaggagcg actcgatggg gaccatggag tccgacacgc gccgctggcc cagctcgtag
      781 acgagggact cggccgtgtc gacggccttc tccacgtcgt cgggcacgtc gtagccgagc
      841 tcggcgatct cgccgcccac gccgatgagc ttgcgcagca ccgcgtggtc gctgatgatc
      901 cgggcgtagc gggcggcgtt ggacaccgcc ggggcggtgg agacgatctc ggccagcagg
      961 ctgcgcccgc cgatggcctc gagcacgccg gcccgagcca gctcgtcggc cacggtgacg
     1021 gggtcggccg gctcgccggc agagtccagg ctgaggatgg cgtcgaagat gtgggcgtgc
     1081 gccggcttgg agaagtccgc gggccgcagc atcgtcgacg tcgccgagat ggcgtccttg
     1141 gacagcatca tcgccccgag caggttctgc tcggccacca ggtcgctcgg gggcacgcgg
     1201 ccggcaccgc cgggacggga tccaccgccg ttgccgcggc caggggccgg ggccctcgag
     1261 ggggcatcgc tgcgagtcgt cgtgtcggtc aaacgagggg accttccgtg gcaggaggtg
     1321 ggcggcgttc accgtcgcgc catctccctg tgggtcgcta gtggtgtgcg ctggggaaag
     1381 gcctgtggat tctgtggaga agccctggtc aacgggcgcg tcgaagcaga gctggtgtcg
     1441 tgcaggaggt gatggggtgc ggccccccgg ccgcaccccg agtggcgcta gagcgcctcg
     1501 acctcgatcg tgatggggaa ctgcacctct tggtgcagct tggccatcac gtagtgcgtg
     1561 ccgaccgtct tgatcggctc atcgatgatg atgatcttct tgtcgagctc gatctcggtc
     1621 tgggccttga cggccgtggc gatctcggac tcgtggacgg cgccgaacag gtggccgttc
     1681 tcggccgcct tggcggtgat gtggatggtc tggggcacga gcgcggtggc gacctcctgg
     1741 gccgcctcga tggccttggt gtcgcggacc tcgcgcgcct tgcgcatctg ctcggcctgg
     1801 agctcggcgt cggccgtggc ccgcatggcg agccccttgg ggatgaggtg gttgcgggcg
     1861 tggccgtcgc tgacgtcgac cagatcgccg cgcttgccga ccatgtcgac atcggtgcgc
     1921 aggatcaccc tcatgcctcg acctcctggt cctcgctgac ctcgtcggtc gtctcggccg
     1981 actcggtgac ctcggtggac tcgtccacgt cgtcgctgaa ctcctcgtcg gagcgctcgt
     2041 cgcggcgcgg gccacgacgg tcgtcgccac ggccacggcc gccgccacgc tgctgggtga
     2101 cgcggctggg gtagggcagc agcgccatct cgcgagcgtt cttgatggct gcggcgatgt
     2161 cggcctgctg ctgggtgtcg ttgccggtga cgcgacgggc ccggatcttg gcccggtcgg
     2221 actggaaccg gcggagcagg ttcacgtcct tgtagtcgac gtaggagacc ttctcctgga
     2281 tgaggatgct cgtg
//
LOCUS       DATISU010000454         1806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_789536, whole genome shotgun sequence.
ACCESSION   DATISU010000454 DATISU010000000
VERSION     DATISU010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789536"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /gene="disA"
                     /locus_tag="VLR27_18085"
     CDS             <1..567
                     /gene="disA"
                     /locus_tag="VLR27_18085"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HSP05419.1"
                     /translation="SATLSALEVEDLVTVRDVVTVLQRTEMVRRIADELASDIVELGT
                     DGRLVRLQLDELMGGVDDERRQVVRDYFHEESSWHLDEAMTALADLGTEDLLDLRTVS
                     SMLHLPGTLDLDASVQPRGYRLLSRIPRLPDSIIEAIVERFGSLQKIMRATIDDLDDV
                     GGVGETRARSIKDGLARLAESSILDRYS"
     gene            complement(608..1330)
                     /locus_tag="VLR27_18090"
     CDS             complement(608..1330)
                     /locus_tag="VLR27_18090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSP05420.1"
                     /translation="MEQRRVELTTPDGEMTTFVFHPTHGGPHPVVLYLMDAPSIRPAL
                     MDMASRLATAGYYVLLPFLFYRGMEYRELGTSDEDMHKRQELMGTVTPTNILGDAEAL
                     LAFADDDPAAADGPVGAVGFCMSGGLVVSIARAMPDRVKAGASIHGAWLVRDGDDSPH
                     RGLDAVDAELHFAWCADDPTAPVEDREVLEQALRDAGVTYTLDWHDDAVHGFAPPGPR
                     YHRDASELHWERVHSLLRRRIG"
     gene            complement(1333..>1806)
                     /locus_tag="VLR27_18095"
     CDS             complement(1333..>1806)
                     /locus_tag="VLR27_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSP05421.1"
                     /translation="SADGRESVIALMERGDLFGEMPLFDGLGRSTEARALEPSIVTVV
                     PYEPVQKIYEERPALLWLVVALLSTRLRITDSALADSVFLDVTGRTAKRLLELAGDQD
                     EFTLPVTQEELAGMVGASRERVNKAIASFLRLGWLDQRDRRYRITDREQLTRRAT"
BASE COUNT          259 a          644 c          652 g          251 t
ORIGIN      
        1 tcggccaccc tctccgccct cgaggtcgag gacctggtca cggtgcgcga cgtcgtcacc
       61 gtcctgcagc gcaccgagat ggtgcgccgc atcgccgacg agctcgcctc cgacatcgtc
      121 gagctcggca ccgacggccg cctggtgcgc ctccagctcg acgagctcat gggcggcgtc
      181 gacgacgagc ggcgccaggt ggtgcgcgac tacttccacg aggagagcag ctggcacctc
      241 gacgaggcca tgacggccct cgccgacctc ggcaccgagg acctgctcga cctgcgcacc
      301 gtgtcctcga tgctgcacct gcccggcacc ctcgacctcg acgcctccgt gcagccccgc
      361 ggctaccgcc tcctgtcgcg catcccccgc ttgccggact ccatcatcga agccatcgtc
      421 gagcgcttcg gctcgctgca gaagatcatg cgggccacca tcgacgacct cgacgacgtg
      481 ggcggcgtgg gcgagacccg ggcccgttcc atcaaggacg gcctggcccg cctcgccgag
      541 tccagcatcc tcgaccgtta ctcctgagcg aactggcagc ggccacggaa cagggctgcc
      601 gtcggcgtca gccgatgcgg cgacgcagga gcgagtggac ccgctcccag tgcagctcgg
      661 aggcgtcccg gtggtaccgg ggtccgggcg gggcgaagcc gtggacggca tcgtcgtgcc
      721 agtcgagcgt gtaggtgacc ccggcgtcgc gcagcgcctg ctccagcacc tcgcggtcct
      781 cgaccggggc ggtggggtcg tcggcgcacc aggcgaagtg cagctcggcg tccacggcat
      841 cgaggccccg gtggggggag tcgtcgccgt cgcgcaccag ccaggcgccg tggatcgacg
      901 cgcccgcctt cacccggtcg ggcatggccc gggcgatcga cacgacgagg ccgccgctca
      961 tgcagaaccc gacggcaccg accgggccgt cggctgcagc cgggtcgtcg tcggcgaagg
     1021 cgaggagcgc ctcggcgtcg ccgaggatgt tggtgggcgt gacggtgccc atcagctcct
     1081 ggcgcttgtg catgtcctcg tcgctggtgc cgagctcgcg gtactccatg ccccggtaga
     1141 acaggaacgg cagcagcacg tagtacccgg cggtggcgag gcgggacgcc atgtccatga
     1201 gggccgggcg gatgctcggc gcgtccatca ggtacagcac cacggggtgg ggcccgccgt
     1261 gggtggggtg gaagacgaag gtcgtcatct ccccgtcggg ggtggtgagc tcgacccggc
     1321 gctgctccat gctcaggtgg cccggcgggt gagctgctcg cggtcggtga tgcggtagcg
     1381 gcggtcgcgc tggtcgagcc agccgaggcg caggaacgag gcgatcgcct tgttgacccg
     1441 ctcgcgagag gcgccgacca tgccggccag ctcctcctgg gtcaccggga gggtgaactc
     1501 gtcctggtcg ccggccagct cgagcagccg cttggccgtg cgaccggtga cgtcgaggaa
     1561 caccgaatcc gccagtgccg agtccgtgat gcgcaggcgg gtcgacagca gcgccacgac
     1621 cagccacagc agggccggcc gctcctcgta gatcttctgc accggctcgt aggggaccac
     1681 ggtgacgatc gagggctcga gggcacgggc ctcggtggac cggccgagcc cgtcgaacag
     1741 gggcatctcg ccgaacaggt cgcctcgctc catcagcgcg atgacggact cccgcccgtc
     1801 ggccga
//
LOCUS       DATISU010000455         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_277163, whole genome shotgun sequence.
ACCESSION   DATISU010000455 DATISU010000000
VERSION     DATISU010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277163"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1417
                     /gene="proS"
                     /locus_tag="VLR27_18100"
     CDS             <1..1417
                     /gene="proS"
                     /locus_tag="VLR27_18100"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HSP05422.1"
                     /translation="MAKAPVLTPQAEDFPRWYQDVLNKAELADNGPVRGTMVIRPYGY
                     GLWERMQAEMDLRIKACGAENAYFPLFIPESYLKREADHVEGFSPELAVVTHGGGKEL
                     EEPIVVRPTSETVIGEYMGKWIQSYRDLPLLLNAWGNVVRWELRPRLFLRTTEFLWQE
                     GHTAHATREDAAAYAAKILEDAYRAFMVDILAMPVLVGIKPSYDRFAGAINTLACEGM
                     MRDGKALQMGTSHELGQNFAKAFDISFLDADSQQQLCWTTSWGSSTRMVGGLIMCHGD
                     DRGLRVPPRLAHIQAVVMVIRDEDGAGGVAAGIHDELVRAGVRSRLDDRVDTAFGRRS
                     TDWELKGVPIRLEIGPRDVASGQAVLVRRDTGEKTPVPITEITSTVQRLLEEIQADLL
                     AEATTMRDDRTADVDSVEGVLEAASTGFARVPWPVVAGEQAAARLGTKAVTVRCIQRE
                     DGSVPDNEDEEGLYAICARSY"
     gene            1483..2064
                     /gene="rimP"
                     /locus_tag="VLR27_18105"
     CDS             1483..2064
                     /gene="rimP"
                     /locus_tag="VLR27_18105"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HSP05423.1"
                     /translation="MFLDPDTPEEVHAMAKTDLVRSLVEPLAAEAGADIYDVTLAGGK
                     LVVALTRSGGIDLETLTKVSRDLNAVLDEQDPISGSYTLEVTSPGLERNLRTAEHFAG
                     AVGDEVTIRTNPQVEGERRVRGTLRAADDTTVTVELEEGGERTLDIADVERARTVFTW
                     GNPDKPGKKPTKKPAKKKSPQQKTASSKESRST"
     gene            2061..>2308
                     /locus_tag="VLR27_18110"
     CDS             2061..>2308
                     /locus_tag="VLR27_18110"
                     /inference="COORDINATES: protein motif:HMM:NF020118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HSP05424.1"
                     /translation="MSNPDMFEALQALAAEKGISVDTLMSALADALESAYKRMPGALE
                     YAWVTIDPATFDIRVYGQELDEDGEPEGDVFDVTPENFG"
BASE COUNT          386 a          861 c          762 g          299 t
ORIGIN      
        1 catggccaag gcacccgtgc tcacccccca ggcggaggac ttcccccgct ggtaccagga
       61 cgtcctcaac aaggcggagc tggccgacaa cgggccggtg cggggcacga tggtcatccg
      121 cccctacggg tacggcctgt gggagcgcat gcaggccgag atggacctgc gcatcaaggc
      181 ctgcggggcc gagaacgcct acttcccgct cttcatcccc gagagctacc tcaagcgcga
      241 ggccgaccac gtcgagggct tcagtcccga gctggccgtg gtcacccacg gcggcggcaa
      301 ggagctcgag gagcccatcg tcgtgcgccc cacctccgag acggtcatcg gcgagtacat
      361 gggcaagtgg atccagagct accgcgacct tcccctgctg ctgaacgcct ggggcaacgt
      421 cgtgcgctgg gagctgcgcc cccgcctgtt cctgcgcacc accgagttcc tctggcagga
      481 gggccacacc gcccacgcca cccgcgagga cgccgccgcc tacgccgcca agatcctcga
      541 ggacgcctac cgcgccttca tggtcgacat cctcgccatg cccgtgctcg tcggcatcaa
      601 gcccagctac gaccgcttcg ccggggccat caacaccctg gcctgcgagg ggatgatgcg
      661 cgacggcaag gcgctgcaga tgggcaccag ccacgagctg ggccagaact tcgccaaggc
      721 cttcgacatc agcttcctcg acgccgacag ccagcagcag ctgtgctgga ccacgtcgtg
      781 gggcagctcc acccgcatgg tcggcgggct catcatgtgc cacggcgacg atcggggcct
      841 gcgggtgcct ccccgcctcg cccacatcca ggccgtggtg atggtgatcc gcgacgagga
      901 cggcgccggc ggcgtcgccg cgggcatcca cgacgagctg gtgcgtgccg gcgtgcgctc
      961 ccgcctcgac gatcgggtcg acaccgcctt cggccggcgc tccaccgact gggagctcaa
     1021 gggcgtgccg atccgcctgg agatcggtcc ccgcgacgtc gccagcggcc aggccgtgct
     1081 ggtgcggcgc gacacgggcg agaagacccc cgtgcccatc accgagatca cctcgaccgt
     1141 ccagcgcctg ctggaggaga tccaggccga cctgctggcc gaggccacca cgatgcgcga
     1201 cgaccgcacc gcggatgtcg actccgtcga gggcgtgctc gaggccgcgt ccaccggctt
     1261 cgcccgggtg ccgtggcccg tcgtggccgg cgagcaggct gcggcccgcc tcgggaccaa
     1321 ggcggtcacc gtgcgctgca tccagcgcga ggacggcagc gttccggaca acgaggacga
     1381 agagggcctc tacgcgatct gcgcccgttc gtactgaacg gggtagagtc gcctgcgaac
     1441 tcgttcctgc atcggagcgt gggcctcggg cccacgcttc tcttgtttct ggaccccgac
     1501 acgcccgagg aggtgcacgc catggccaag accgatctcg tccgcagcct ggtcgagccc
     1561 ctcgccgccg aggccggggc cgacatctac gacgtgaccc tcgccggcgg caagctcgtc
     1621 gtcgcgctca cccgcagcgg cggcatcgac ctcgagaccc tcacgaaggt gtcgcgggac
     1681 ctgaacgccg tcctcgacga gcaggacccc atcagcggct cctacaccct cgaggtcacc
     1741 agccccggcc tcgagcggaa cctgcgcacg gccgagcact tcgccggtgc cgtgggcgac
     1801 gaggtcacca tccgcaccaa cccccaggtc gagggcgagc gccgggtgcg tggcaccctg
     1861 cgcgccgccg acgacaccac ggtcacggtg gagctcgagg agggcggcga gcgcacgctc
     1921 gacatcgccg acgtcgagcg ggcccgcacg gtgttcacgt ggggcaaccc cgacaagccc
     1981 ggcaagaagc cgacgaagaa gccggccaag aagaagtccc cgcagcagaa gaccgcatca
     2041 agcaaggaat cccgatcgac atgagcaacc cggacatgtt cgaggccctc caggcgctgg
     2101 ccgccgagaa gggcatctcc gtcgacaccc tgatgtctgc cctcgccgac gccctggagt
     2161 ccgcctacaa gcggatgccg ggcgccctcg agtacgcctg ggtcaccatc gacccggcga
     2221 ccttcgacat ccgcgtctac ggccaggagc tggacgagga cggcgagccc gagggcgacg
     2281 tcttcgacgt gacccccgag aacttcgg
//
LOCUS       DATISU010000456         4099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_371016, whole genome shotgun sequence.
ACCESSION   DATISU010000456 DATISU010000000
VERSION     DATISU010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371016"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="VLR27_18115"
     CDS             <1..1278
                     /locus_tag="VLR27_18115"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HSP05425.1"
                     /translation="AWFDMIEPFADYAFNKSHSYGYGFIAYQIAYLKANYPAEYMSAL
                     LTSVKSNLDKAAVYLAECRAMEIPVTVADVNRSLVDFAPELRRGDDGGIDGAEIVFGM
                     SAVRGVGTGLCHLIIAEREANGAYADFYDFCERVDLGVLNKKTVEALIKAGAFDSVGH
                     PRQGLLMVFESIIDRTIARRKEAEAGVMSLFDSMGGDDVSTSGGGGFGFDEREEIPDV
                     EFPKAQKLAFEKEMLGLYVSDHPLFGAEDFLARRTELGLDALAECEDGAIKTVGGVIT
                     NLQRKWTKKGDLMGVFVLEDLESNVETMVFPRTMTEHGHKLEDDAIVLVKARIDKRDD
                     MPKLIAMEIEVVEGVSDSATPFRLRVMPNRLTAELIAQLKEILLEHPGDHPVLLHLGD
                     DKVLRLPPAYGVDAETGLVSEIRVLLGPAALVE"
     gene            1482..2087
                     /locus_tag="VLR27_18120"
     CDS             1482..2087
                     /locus_tag="VLR27_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05426.1"
                     /translation="MADLCAESPFPHEIGAITKEAESWVLVSQAREDGKLKGFTFSTL
                     ERIGGTPCVLIGMGSIKRSSKRDTVLRAIMQEELRRAVLAFPDEDVLVAARLTDPSGY
                     EAFKALTDLIPRREHKASGEERAWGRRLAKRYGIVPSRYADRQFRVKGDGSLPVVFDH
                     ECVKRERVAEYEEFFADLDPESGECVIAFGWAMAEDLEKLA"
     gene            2033..3154
                     /locus_tag="VLR27_18125"
     CDS             2033..3154
                     /locus_tag="VLR27_18125"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSP05427.1"
                     /translation="MRHRLRLGHGRGPREAGVGAEPPGEATHEHGDLRRRSRHPKGRR
                     AWPRRGGLNLSTALVLGAGGTVGMAYHAGVLRALEEVGGFRPDDADLVVGTSAGSMVG
                     AMVRSGLTTDDLYRASLGEHPHLGQHDQRSPWAAAWDGPVDAMRRLLGSMYVLQRSAL
                     RLPVPPLPAFLRRAFPGGFFTIDDAEDTMAEVLPTEWPERPLWLVTVDVRTGRRVVLG
                     SRDPVRTDLHTAVKASCAIPAFFQPQRVRGRTLVDGGVHSTSNLDLATGIAADVVIGI
                     VPMAFDTAPRPALVHQVGRLHAHRRLERELAEVRATGAAVLLLRPTEDDLDAFGGLNM
                     MRRHGNERVTRVAYESASRQLSTPYARSVLDRLQDTVTQ"
     gene            3151..3795
                     /locus_tag="VLR27_18130"
     CDS             3151..3795
                     /locus_tag="VLR27_18130"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HSP05428.1"
                     /translation="MTTLDDLARLLSARHMVRRYADEPVPDDLLGRLLDLARRAPSAG
                     NTQSWSFLVLRDADAERYWATTMPDPDARARFRWQGLLRAPVLVVPYVRPAAYAERYG
                     EPDKSGTGLGAGTDAWPVPYWWVDGGAVVQNLLLLATAAGLGACLFGQFDHEAAVRTA
                     FGVPDDQRAVGTIALGWPAPDEPGRSAGRPRPALDDVVHRGFWQRERNDGRNDG"
     gene            complement(3838..>4099)
                     /locus_tag="VLR27_18135"
     CDS             complement(3838..>4099)
                     /locus_tag="VLR27_18135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05429.1"
                     /translation="VPSAFGVLLGAIDLHHTGTTEIVVAGDRPDLLDAVRPRWMPNAV
                     LAWGERYESPLWTGRDDGRAYVCRDYTCQLPAGTPEELTAQL"
BASE COUNT          595 a         1536 c         1377 g          591 t
ORIGIN      
        1 gcctggttcg acatgatcga gcccttcgcc gactacgcct tcaacaagtc ccactcctac
       61 ggctacggct tcatcgccta ccagatcgcc tacctcaagg cgaactaccc ggccgagtac
      121 atgtcggccc tgctcaccag cgtcaagagc aacctcgaca aggccgccgt gtacctggcc
      181 gagtgccggg ccatggagat cccggtcacc gtggccgacg tcaaccgctc cctggtcgac
      241 ttcgcccccg agctgcggcg cggcgacgac ggcggcatcg acggagccga gatcgtcttc
      301 ggcatgtccg ccgtgcgcgg cgtcggcacc gggctctgcc acctcatcat cgccgagcgc
      361 gaggccaacg gcgcctacgc cgacttctac gacttctgcg agcgcgtcga cctcggcgtg
      421 ctcaacaaga agacggtgga ggccctcatc aaggccggtg ccttcgactc ggtcggccac
      481 ccccgccagg gcctgctgat ggtgttcgag tcgatcatcg accgcaccat cgcccgccgc
      541 aaggaggccg aggccggcgt catgagcctc ttcgactcga tgggcggcga cgacgtcagc
      601 accagcgggg gagggggctt cgggttcgac gagcgcgagg agatccccga cgtcgagttc
      661 cccaaggccc agaagctggc cttcgagaag gagatgctcg ggctctacgt gtccgaccac
      721 ccgttgttcg gtgccgagga cttcctggcc cgacgcaccg agctcggcct cgatgccctc
      781 gccgagtgcg aggacggcgc catcaagacc gttggcggtg tcatcaccaa cctgcagcgg
      841 aagtggacca agaagggcga cctgatgggc gtcttcgtgc tcgaggacct cgagtccaac
      901 gtcgagacga tggtcttccc ccgcacgatg accgagcacg gccacaagct cgaggacgac
      961 gccatcgtcc tggtcaaggc tcgcatcgac aagcgcgacg acatgcccaa gctcatcgcc
     1021 atggagatcg aggtcgtcga gggcgtctcc gactcggcca cgccgttccg gctgcgggtc
     1081 atgcccaacc gcctcaccgc cgagctgatc gcccagctca aggagatcct cctcgagcac
     1141 ccgggcgacc acccggtcct gctccacctc ggcgacgaca aggtcctgcg cctcccgccc
     1201 gcctacggcg tggacgcgga gaccggtctg gtgtcggaga tccgggtcct cctcgggccc
     1261 gcagccctgg tggagtaggg gtccggctcc gccgactcga gttgctcggc agagcctcgc
     1321 aacatcggcc gcacagctgt tcggcgtgat ttgcgcggta cccctcacag cgagtggact
     1381 ggagcgacca caaggccggc aacacggggg cttccacacg catgtcgatc gaggtcgaaa
     1441 cgaaggactg cacggggctc agcgatgtcg agctggccga attggctgat ctctgcgctg
     1501 agagcccgtt cccgcacgag atcggggcga tcaccaagga ggccgagtcc tgggtgctcg
     1561 tctcccaggc tcgcgaggac ggcaagctca agggcttcac cttctcgacc ctcgagcgca
     1621 tcggtggcac cccctgcgtg ctcatcggca tgggctcgat caagcggtcg tccaagcgcg
     1681 acaccgtgct gcgggcgatc atgcaggagg agctgcgccg ggccgtgctg gccttcccgg
     1741 acgaggacgt cctggttgcc gcccggctca ccgatccctc cggctacgag gccttcaagg
     1801 ccctcaccga cctcatcccg cgccgtgagc acaaggcgtc gggcgaggag cgggcctggg
     1861 gtcgtcgcct cgccaagcgc tacggcatcg tcccgtcccg ctacgccgac cgccagttcc
     1921 gggtgaaggg cgacggctcg ctgcccgtgg tgttcgacca cgagtgcgtc aagcgggagc
     1981 gggtcgccga gtacgaggag ttcttcgccg acctcgaccc cgagtccggc gagtgcgtca
     2041 tcgccttcgg ctgggccatg gccgaggacc tcgagaagct ggcgtaggag ccgagccgcc
     2101 aggcgaggcg acccatgagc acggtgatct acggcggcga agccgccatc ccaagggccg
     2161 cagggcgtgg ccgcgtcgcg gaggtctcaa cctgagcact gcgctcgtcc tcggcgccgg
     2221 cggcacggtg ggcatggcct accacgccgg tgtgctgcgg gcgctcgaag aggtcggtgg
     2281 cttccgcccc gacgacgccg acctcgtcgt cggcacctcc gccggctcca tggtcggcgc
     2341 catggtccgc agcgggctca ccaccgacga cctctaccgg gcctcgctcg gtgagcaccc
     2401 gcacctcggc cagcacgacc agcgcagccc gtgggctgcg gcatgggacg gaccggtcga
     2461 cgcgatgcgt cgcctcctcg gctccatgta cgtgctgcag cgctcggcgc tgcgcctgcc
     2521 ggtgccgccg ctgccggcgt tcctccggcg ggccttcccg ggtggcttct tcaccatcga
     2581 cgacgccgag gacacgatgg cggaggtgct gcccaccgag tggcccgagc gccccctgtg
     2641 gctggtgacc gtcgacgtcc gcaccggtcg ccgggtcgtg ctcggcagcc gtgacccggt
     2701 ccgcaccgac ctccacaccg cggtcaaggc gtcgtgcgcc atcccggcct tcttccagcc
     2761 gcagcgggtg cgcggccgca ccctcgtcga cggcggcgtc cactccacca gcaacctcga
     2821 cctcgccacg ggcatcgccg ccgacgtggt catcggcatc gtccccatgg ccttcgacac
     2881 cgcccctcga cccgcgctcg tgcaccaggt cggccggctc cacgcccacc ggcgcctcga
     2941 gcgcgagctg gccgaggtcc gggccaccgg cgccgccgtc ctgctgctgc gacccaccga
     3001 ggacgatctg gacgccttcg gggggctgaa catgatgcgc cggcacggca acgagcgggt
     3061 cacccgggtg gcgtacgagt ccgcgtcgcg ccagctgtcc acgccctacg cccgcagcgt
     3121 cctcgaccgc ctccaggaca ccgtcaccca gtgacgaccc tggacgacct ggcccggctg
     3181 ctctcggccc gccacatggt caggcgctac gccgacgagc cggtccccga cgacctgctc
     3241 ggacggctgc tggatctcgc tcgacgggcg ccgtcggccg gcaacaccca gtcctggtcg
     3301 ttcctggtgc tccgggacgc cgacgccgaa cgctactggg ccacgacgat gcccgacccc
     3361 gacgcccgcg cccgcttccg ctggcagggc ctgctccggg ccccggtcct cgtcgtgccc
     3421 tacgtgcgtc ccgcggccta tgccgagcgc tacggcgagc cggacaagtc cggcaccggc
     3481 ctcggtgccg gcaccgacgc ctggcccgtg ccgtactggt gggtggacgg gggagcggtg
     3541 gtgcagaacc tgctcctcct ggccacggcg gccggcctcg gcgcctgcct cttcggccag
     3601 ttcgaccacg aggccgccgt gcgcactgcg ttcggcgtcc ccgacgacca gcgcgccgtc
     3661 ggcaccatcg cgctcgggtg gcccgccccc gacgagcccg gccgaagcgc cggccgcccc
     3721 cgtcccgcgc tcgacgacgt cgtccaccga gggttctggc agcgagagcg gaacgacggc
     3781 cggaacgacg ggtgaagccg tcgcgctgga tcgtcgttcc gccttcgctg ccagaaccta
     3841 gagctgggcg gtgagctcct cgggggtccc cgccggcagc tggcacgtgt agtcccggca
     3901 gacgtaggcc cggccgtcgt cgcggcccgt ccagaggggc gactcgtagc gctcgcccca
     3961 ggcgagcacg gcgttgggca tccaccgggg ccggacggcg tccagcaggt cggggcggtc
     4021 gccggcgacg acgatctccg tggtgcccgt gtggtgcagg tcgatggcgc cgagcagcac
     4081 gccgaaggcg gacggcacc
//
LOCUS       DATISU010000457        15533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_510446, whole genome shotgun sequence.
ACCESSION   DATISU010000457 DATISU010000000
VERSION     DATISU010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 15533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15533
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510446"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VLR27_18140"
     CDS             <1..1243
                     /locus_tag="VLR27_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSP05430.1"
                     /translation="SRIYYQGKFYDYPINVVNALKNLGPVEAVRCGLSFLWVRVRPPK
                     DLTTLEGYIVSNYGRRLYEHFFETYSTKVWGVPPSEMSADWGAQRIKGMSLWDAVWEP
                     IRGRFSRRDRSKQVTSLIEEFQYPKYGPGMMWERCHELVEGQGSKVQFRAKVTRVEHR
                     DGRAVTVTSTDDSGAETTHDADHVISTMPFPALLRAMDPPPPAEVLAAADDLHFRDFL
                     SVSLVVSEEKVDWNDNWIYIHDPEVKTMRIQNFGSWSPYLVKDGRNVLGLEYTVDEGD
                     EWWTATDEHLIQTGTEELVRLGLIQSSDVEQGYVVRMPKAYPVYDEDYQRNVGVLRRW
                     LEANVSNVHPVGRNGMHRYNNQDHSMMTAMLTVENIYGAEHDIWEVNVEAEYHETSEE
                     GSTTSGSSGTGRDAPILPKRT"
     gene            1269..3311
                     /locus_tag="VLR27_18145"
     CDS             1269..3311
                     /locus_tag="VLR27_18145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HSP05431.1"
                     /translation="MTAPAAPSRQRSTGFAHRPGLDGLRGLAVAGVLAFHSGFAWATG
                     GYLGVSAFFTLSGFLITTLLLGEQRGTGRVDLRAFWIRRFRRLLPAALLGVLAATAYV
                     LAFADQNGLDRFRGDALAAIGYVANWRFVLSDLSYAELFTGDSPLQHYWSLAIEEQFY
                     LVLPLLAAVLLRRRSGWRGLTVLAVAGIVASVIATLVVGGGDRAYYGTDTRMAELLVG
                     VLLAIWHHRRDLGTSLPEGVRSAAGAAALAAMLGLWVATDQSSGALHPWVLLGHAVLT
                     AAIIVSVLAPTPVARVLSLRPLTELGRISYGVYVYHWPVFLWLSPQRTGLDGGGIDDV
                     LLAGLRILVTLAIAVPSHRFVEQPIRHGGLHLGERRGPLLTAGAVAAVALVAVVPATV
                     IDRAPLIDFAAAEDLQDQLLDSPRPTTTTTTTAPPPAGPAESATTTTTAPPGPPPRVA
                     LFGDSTAQLFIPGLIDWAIETQRMDFRSAGTISGCGLGRGGERRNLDSSVEQVPDGCD
                     FTMAWREAISIATPQIAVVEIGLWDMTDRRIPGDDEWRTIGDPVYDEFLRSELRTATD
                     FLLETAGSVIWVLQPPPVRSTTEPGDPIFERLDRWNAMLVDELGDHPQVGFVDLEDWL
                     ASTSEGTDSLRLRPDGTHFSTETSVELAQWLGPAILEAARTHDVVGADDVDGASGP"
     gene            3308..4612
                     /locus_tag="VLR27_18150"
     CDS             3308..4612
                     /locus_tag="VLR27_18150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSP05432.1"
                     /translation="MTEPERWHPDTRWRAGLTGVFLVALAARVTYAIAAAPNILPISD
                     AGGYRLLGQHLAEGLGYIRPYDFARADIVRPTAEFPPGFPSLLAVFHTVGLRSVPAQR
                     LALAVLGALAVVGIALLAQRWFSRRGALVLGALAAVHPALFAVESALLAESLFLWLVV
                     LVLLAVVRFRESPSPGRAVAIGLLGGAATLVRSEGIVLALVLVIPLLVTAPPRRFRVG
                     AGVAVLVAALLLPGAWAVRNLRTFEEPVLVSNNLGSVLSGANCDRTYDSELIGFWYIS
                     EDCFDGFRQEALEVADESVVAKGLRDDGLTYLRDHLGEVPKVAAVRVLRTFQMYEPEQ
                     QGRLATFEGRRLLTERITGWILWATLPLALAGALHLGRARRWVDLWLLTAPIGVVVLV
                     AAATYGNPRFRIAAEVPLLILAGLGIQAGVAAWRQRRQAAEA"
     gene            4609..6639
                     /locus_tag="VLR27_18155"
     CDS             4609..6639
                     /locus_tag="VLR27_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05433.1"
                     /translation="MTTAVAPAVDDGDEQEVRRAYRIGALVGLLAFVVVLMGGQGTLL
                     PWVPAGDFYDAQAESFLDGRLQVDRDVLGIEAFEHDGGTYMYQPPWPAVLRLPVVALT
                     DRFDGRLGAPSMLLAHVVLLVAAERVLLEARRAIPGAAAGGRLGRALCALFGLTLAGG
                     STATFLASRPWVYHESAMWGMAWTVVTFAGLLVLRRRPSWGALAVTALGCGGAVMSRA
                     SVGAGACVAVGVLALAALAAPGRRVLDRLRGPAGAVPLLAVAAVPAAVYAALNTLKFG
                     NPTSIPFEAQGYTQVSAARREMLAEAGGSLFGLRFAPTTLWHYLRPTGIRVVDQLPFV
                     DFPPAGGPVVGDVTFDLVDHTSSITVTLVALVVAAAVGAWALVRRGPTDRAAWFAVAL
                     GGLAGCATIVPFGYVAQRYHADVIPFLVPMAALGVHAVSRTPGRTRRRTSGIVGIGLV
                     LVTLWFQIGLATVYQRLRAPNPAPAQVAGLLEARLDIGADPDVRVAETLPDAAPRDAV
                     VVIGDCRGVYVWSAAPPSDIYPPGWVAAERTRAGGRVELAVDLSQLEPGRRHALVHWT
                     EAPVVAWVTPEDGQVVVGITAGDLEYPSRSVDLGDGTVELEVYLDPALGEGGVRWRGR
                     LLTNGFLPRLDGPATLRIGEPPVGSDYAPSATSEVGSSDGALCARLVDAAAE"
     gene            complement(6558..8072)
                     /locus_tag="VLR27_18160"
     CDS             complement(6558..8072)
                     /locus_tag="VLR27_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HSP05434.1"
                     /translation="MAEVPSPRVGVLVVAYNAASTLASVLDRIPEGYRESIDHVMVAD
                     DASTDSTFLIGVGYRETFAHLPITVVRHDENRGYGGNQKWGYRWAMEHGLDVVVLLHG
                     DGQYAPELLPDMVAPIVDGGADAVMGSRMLPPGSARAGGMPLYKWVGNRILTTVQNRL
                     TGASLSEWHSGYRAYRVDALRDLDLDTMSDGFDFDTQILLGLMGAGRSIVEIPIPTYY
                     GDEISYVNGMRYARQVTGHVLRHRLRRIGFGSGTRGVGYEAKVGAGTSHQLLLERLSG
                     HPAGRVLDLGCADGHLGGALRERGHEVIGVDGEASPEAARRLDRHVVADLDAGLPDEV
                     TGDFDVVLVADVLEHLRDPASLLEEVHGRLRPGGRVVGSIPNFAHWYPRVRVASGLFD
                     YDARGILDRGHLRFYTRRSFRRLCEDAGYRVADLDAAGLPVEVFQRGGAGVVGPVLSA
                     IGAIDRALARVRPTLFAFQFLFELEPAGHSAAASTSRAQSAPSDDPTSLVAEGA"
     gene            complement(8082..10532)
                     /locus_tag="VLR27_18165"
     CDS             complement(8082..10532)
                     /locus_tag="VLR27_18165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HSP05435.1"
                     /translation="MVATSTSSARARPTIPHVEALDGLRGVAVIGVLLFHADHLAGGF
                     LGVDLFFTLSGFLITSLLVVEHRERGRISLRAFWARRARRLLPAMLAVVAAATVVATL
                     TLPSAEMERFGNQALLTLAYVANWGEIASSRDYWALFDAPTPLEHVWSLAIEEQFYVV
                     WPLLVLGSVRLLGRRVGIRALVGAGLVASLVVSWVVVEGAGGVTRAYYGTDTRAASIL
                     VGAALATITAGASRRPSGVERGVAAAAVASLGALLASWVLVDGTELWLYRYGLLAHAV
                     LTAVVIAAVSGPFGGRLGQVLRVRPLVRIGILSYGLYLWHWPVYVVLDPDRTGLEGWS
                     LTGLRLAVSIAIATASYHLLEAPIRSQRWRIPRPALVSAGAVAATAALVVVLVWQAGP
                     SAEERTSDLAVGAAGTEATAVAGRLLVIGNPGTVTLAREVASAGEAQQLRVRVEGEIG
                     CVDCTGWSQRWSSVVAREQPDVVLLLAGWAGTDDRDLGDGVLRGPCDPMYDAHHREEL
                     RDVVDVLGSSGAVVVLATTAPTADGTVDKSSGCLDRISDEVTRGFPSELLDLARMEPA
                     GARSTARWILDGLSRLTGLDQLPFVVVVGDSQALRLVEHAPPFDELDLRVGNLGALGC
                     GLSTQSVVMGGQDIDKAGCGPGLAALPDDLAALEPDAVVVHAGVWEVLDQATDAGRIG
                     FEDPRWADRLRANVEATLAGLAAEHHVVALSTPCFSAGSARAALGAEDLDARLAAYNE
                     ILIEEVERLGGTVVDLAGRLCPDGVAVHELDGVVLRPDGIHLERGGAQAVWPWLADEL
                     RAAVRAADPNPGVSSGDS"
     gene            10626..10940
                     /locus_tag="VLR27_18170"
     CDS             10626..10940
                     /locus_tag="VLR27_18170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP05436.1"
                     /translation="MTQTAPNDLAVTHDEDANRYLLRRGDDLVGTLAYRSVGRPDEET
                     INVFSTNISPAVRGQGLGEVLVRGALDDLRTRGTSVMASCWYVADFLDANPDYQDLRP
                     EA"
     gene            complement(10941..11666)
                     /locus_tag="VLR27_18175"
     CDS             complement(10941..11666)
                     /locus_tag="VLR27_18175"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HSP05437.1"
                     /translation="MRVLVIIPTYDEAENIADVVGRVHAAVPDAHVLVVDDNSPDGTG
                     DLVEAMATDDAELHLLRRAGKAGLGSAYRAGFAWGADEGFEVMVEMDADLSHDPAALP
                     SLLARVEDGADLAIGSRYVPGGSIPDWKWHRKALSRWGNRYAGAVLSLDVADATAGYR
                     AYRASALADIDLDSVRADGYGFQIEMADRVARNGGRIDEVPISFTDRTRGTSKMSGRI
                     VVEALVLVTWWGVRRRVPVGRSS"
     gene            complement(11669..12436)
                     /locus_tag="VLR27_18180"
     CDS             complement(11669..12436)
                     /locus_tag="VLR27_18180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP05438.1"
                     /translation="MNDASDPGSPPSLAEVQARVIDVEGWLTDAQIQALHEAALACPP
                     GGRIVEIGSFRGRSTVVLASSAPADVEIVAIDPHAGNDRGPQEIEGFEAEAATDHEVF
                     NRNLSDAGVAERVRHVRAFSDAAHGDVADPIDVLFIDGAHRFGPARADIRDWGARVAP
                     GGRMLIHDSFSSIGVTLAILVELLVSPDWRYDGRAGSLTSYRRTAQSPAARATSALRQ
                     LAQLPWFARNVAIKVLMVTGRHDWARKLGHDGETWPY"
     gene            12566..13747
                     /locus_tag="VLR27_18185"
     CDS             12566..13747
                     /locus_tag="VLR27_18185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSP05439.1"
                     /translation="MPEHPTTDAKIGDLAAAAGIRRIHVLAWRDLDDVEAGGSELHAH
                     EVCREWAAAGLEVTCRTSFAQGHPPEIMRDGYRVLRKAGRYLVFPRSVAAELAGRNGE
                     RDALVEIWNDMPFLSPIWARGPRITFLHHVHGEMWKMVLPPRLAKMGDLLERRLAPPL
                     YRHSRIVTLSESSRDDMLAQLRPRPERVDVVPPGIDPKFSPGEESPTPLVVAVGRLVP
                     SKHVDRLVRAVAHARRSVPDLRLVVVGEGYERLHVEATVEDVGGEAWVDLPGRVTDDE
                     LIELYRRAWVLASASSHEGWGMSITEAAACATPAVVTDIPGHRDAVVDGVTGTLVDAD
                     GDALPERLGDAMAAVLADPERRRRMSDAAAGHAAGFTWRSTATQLMAILADEAGRPRR
                     S"
     gene            13744..>15533
                     /locus_tag="VLR27_18190"
     CDS             13744..>15533
                     /locus_tag="VLR27_18190"
                     /inference="COORDINATES: protein motif:HMM:NF023275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase family
                     protein"
                     /protein_id="HSP05440.1"
                     /translation="MTAPRTPRARDWWLPLAVLAVLVYVPLLLTDPGHVIADTKSYLY
                     LDPGRLLSRAVSMWDPHVGLGTVPHQQIGYLWPAGPWYWVFEQLGIPDWVAQRLWLGT
                     IMVGAGGGVLFLGRTWRWRPTSATAAAFLYALSPFVLTLAARISVLLLPFAGLPWLLA
                     LTVRALHSKGWRHPALFGLIVVTVGSVNATSVLLVGLVPVLWILYSAFGSRDVSTSRA
                     ATTTAKIGVVTLAVNLWWISGLSVQATDGIEVLRYTETAKVVADASVSHEVLRGLGYW
                     FFYGGDRLGPWIEPSVDYTQRLWLLGLTYALPILGLLALGVVRWRHRTFLVGMLLLGT
                     VIAIGAHPWGSPSPIGGFIKAFLLTDVGLAMRSLPRAVPLVALALALAIGAAVGALAE
                     QVTRRGIIGAVAIAGLAYAALPPLWLREMAPVNLQRPEDIPSYWHDAASTLDERDDGT
                     RVLEIPGIDFASYRWGNTVDPVLPGLMDRPYAARELIPYGTPPSADLLNALDLELQEQ
                     TMSDQGLAAVARLMGVGDVVLRNDTQYERYNTPRPRSLLDLVSSMPGFGDPITFGDDV
                     PNVPIEDAPMSDEQPLARDGALPESPAIVT"
BASE COUNT         2026 a         5882 c         5481 g         2144 t
ORIGIN      
        1 gagccgcatc tactaccagg gcaagttcta cgactacccg atcaacgtcg tgaacgccct
       61 gaagaacctc ggcccggtcg aagccgtccg ctgcgggctg tcgttcctct gggtccgggt
      121 ccgtccgccg aaggacctca ccaccctcga gggctacatc gtctccaact acggccggcg
      181 cctctacgag cacttcttcg agacctacag caccaaggtg tggggcgtgc cgccgtcgga
      241 gatgtcagcg gactggggtg cccagcgcat caagggcatg tcgctctggg atgcggtgtg
      301 ggagcccatc cgcggccggt tctcccggcg cgaccggtcc aagcaggtca ccagcctgat
      361 cgaggagttc cagtacccca agtacgggcc cgggatgatg tgggagcgct gccacgagct
      421 ggtcgagggc caggggtcca aggtgcagtt ccgcgccaag gtgacccggg tcgagcaccg
      481 cgacggtcgc gccgtcaccg tcacctccac cgacgactcc ggcgccgaga ccacccacga
      541 cgccgaccac gtcatctcca ccatgccgtt cccggccctg ctgcgggcca tggacccgcc
      601 gcccccggcc gaggtgctcg ccgccgccga cgacctccac ttccgcgact tcctgtccgt
      661 gtcgctggtc gtctccgagg agaaggtcga ctggaacgac aactggatct acatccacga
      721 ccccgaggtc aagacgatgc ggatccagaa cttcgggtcc tggtccccgt acctggtgaa
      781 ggacgggcgc aacgtgctcg gcctggagta cacggtcgac gagggcgacg agtggtggac
      841 cgccaccgat gagcacctca tccagaccgg caccgaggag ctcgtgcgcc tcgggctcat
      901 ccagagcagc gacgtcgagc agggctacgt cgtgcgcatg cccaaggcgt accccgtcta
      961 cgacgaggac taccagcgca acgtgggtgt gctgcgccgc tggctcgagg ccaacgtctc
     1021 gaacgtgcac cccgtgggcc gcaacggcat gcaccgctac aacaaccagg accactcgat
     1081 gatgacggcg atgctcaccg tggagaacat ctacggcgcg gagcacgaca tctgggaggt
     1141 caacgtggag gccgagtacc acgagacctc cgaggagggc tcgacgacct cgggatcctc
     1201 cggcacgggt cgcgacgccc ccatcctgcc gaagcgcaca tgacgacccg acgggtcacc
     1261 cccggccagt gaccgcgccc gctgccccca gccggcagcg gtccaccggc ttcgcccacc
     1321 gtccgggcct ggacgggctg cggggtctcg ccgtcgccgg cgtcctcgcg ttccacagcg
     1381 gcttcgcctg ggcgaccggg gggtacctcg gggtctcggc gttcttcacc ctgtcggggt
     1441 tcttgatcac gacgctgctc ctcggcgagc agcggggcac aggtcgcgtc gacctgcgcg
     1501 cgttctggat ccgccggttc cggcgcctgc tgcccgccgc cctcctcggc gtgctcgccg
     1561 ccaccgccta cgtcctcgcc ttcgccgacc agaacgggct cgaccgcttc cggggcgacg
     1621 cgctggcggc catcggctac gtggccaact ggcgcttcgt gctgtccgac ctgtcctacg
     1681 ccgagctgtt caccggcgac tcgcccctcc agcactactg gagcctggcg atcgaggagc
     1741 agttctacct ggtgctgccc ctgctggccg cggtgctgct gcgtcggcga tcgggctggc
     1801 ggggcctcac cgtgctcgcc gtcgccggca tcgtcgcctc ggtgatcgcg acgctcgtcg
     1861 tgggtggcgg cgaccgggcc tactacggca ccgacacgcg catggccgag ctcctcgtcg
     1921 gcgtgctcct cgccatctgg caccaccgcc gggacctcgg cacctcgctg cccgagggcg
     1981 ttcgcagcgc ggcgggtgcg gccgccctgg ccgccatgct cggtctctgg gtggcgacag
     2041 accagtccag cggcgccctc cacccctggg tgctgctcgg ccacgccgtg ctgacggcgg
     2101 cgatcatcgt ctcggtgctg gcaccgaccc ccgtggcccg ggtcctgtcg ctgcggccgc
     2161 tgaccgagct cggccgcatc tcctacggcg tctacgtcta ccactggccc gtcttcctgt
     2221 ggctctcccc gcagcgaacc ggtctggacg ggggcggcat cgacgacgtc ctcctggccg
     2281 ggctgcggat cctggtcacg ctggcgatcg ccgtgccgag ccaccgcttc gtggagcagc
     2341 ccatccgcca cgggggcctg cacctcggcg agcggcgcgg tcccctgctg accgccggcg
     2401 ccgtcgccgc ggtcgcgctc gtcgccgtcg tgcccgccac cgtcatcgac cgggccccgc
     2461 tcatcgactt cgccgccgcc gaggacctcc aggaccagct cctcgacagc ccgcgaccga
     2521 ccacgacgac gaccacgacc gcccccccgc cggccggacc ggccgagtcg gcgacgacga
     2581 ccacgaccgc cccgcccggg ccaccacccc gggtcgcgct cttcggcgac tccacggccc
     2641 agctgttcat ccccgggctc atcgactggg cgatcgagac ccagcggatg gacttccggt
     2701 ccgccggcac gatcagcggc tgcggcctcg gccggggcgg cgagcgccgg aacctcgaca
     2761 gcagcgtgga gcaggtgccg gacggctgcg acttcaccat ggcctggcgg gaagcgatct
     2821 cgatcgccac cccgcagatc gccgtcgtcg agatcggcct gtgggacatg accgatcgcc
     2881 gcatccccgg cgacgacgag tggcgcacca tcggggatcc cgtctacgac gagttcctgc
     2941 ggagcgagct gcgtaccgcg accgacttcc tgctggagac cgcgggctcg gtgatctggg
     3001 tgctgcaacc gcccccggtg cgcagcacga ccgaaccagg cgatccgatc ttcgagcggc
     3061 tcgaccggtg gaacgcgatg ctcgtcgacg agctcggcga tcacccccag gtgggcttcg
     3121 tcgacctcga ggactggctg gccagcacga gcgagggaac ggactccctc cggctccgac
     3181 ccgacggcac ccacttctcg acggagacct cggtggagct ggcgcagtgg ctcggccccg
     3241 cgatcctcga agcagcccgc acgcacgacg tggtcggcgc cgatgacgtc gacggggcgt
     3301 ccgggccgtg acggagccgg agcgctggca cccggacacc cggtggcggg ccgggctgac
     3361 gggcgtgttc ctggtggcgc tcgccgctcg ggtcacctac gccatcgccg ccgcccccaa
     3421 catcctcccg atcagcgacg ctggtggcta ccggctgctc gggcagcacc tcgccgaggg
     3481 cctcgggtac atccggccct acgacttcgc ccgggccgac atcgtccgcc ccacggccga
     3541 gttcccgccc gggttcccga gcctgctcgc cgtgttccac accgtcgggc tccgctcggt
     3601 cccggcccag cgcctcgccc tcgcggtgct cggcgctctc gcagtggtcg gcatcgcgct
     3661 cctcgcccag cgctggttct cccgccgcgg cgccctggtc ctcggggcgc tggccgccgt
     3721 gcacccggcg ctgttcgccg tcgagtcggc gctcctcgcc gagtcgctgt tcctctggct
     3781 cgtggtgctc gtgctcctcg ccgtcgtgcg cttccgggag tccccgagcc cgggccgcgc
     3841 cgtggcgatc gggctgctcg gcggggcggc cacgctggtc cgctccgagg gcatcgtgct
     3901 cgcgctggtg ctcgtgatcc cgctgctggt caccgcgcca ccgcgacggt tccgagtcgg
     3961 cgccggcgtc gcggtcctcg tcgcggcgct gctgctcccc ggtgcgtggg cggtccggaa
     4021 cctgcgcacc ttcgaggagc cggtgctcgt gtccaacaac ctgggctcgg tgctgtccgg
     4081 cgccaactgc gatcgcacct acgacagcga gctcatcggc ttctggtaca tcagcgagga
     4141 ctgcttcgac ggcttccgcc aggaggccct cgaggtcgcc gacgagtcgg tcgtggccaa
     4201 gggcctgcgc gacgacggcc tcacctacct gcgggaccac ctcggtgagg tgcccaaggt
     4261 ggccgccgtc cgggtcctgc gcacgttcca gatgtacgaa ccggagcagc agggccgcct
     4321 cgccaccttc gaaggccgtc gcctgctcac cgagcggatc accgggtgga tcctgtgggc
     4381 caccctgccc ctggcgctcg ccggcgccct gcacctgggc agggcccgcc gctgggtcga
     4441 cctgtggctc ctgacggcgc cgatcggcgt cgtggtcctg gtcgccgccg ccacctacgg
     4501 caacccccgc ttccgcatcg cggccgaggt tcccctgctc atcctcgccg gactggggat
     4561 ccaggcgggc gtcgcggcct ggcggcagcg acgccaggca gccgaggcat gacgacggcg
     4621 gtcgcccccg ctgtcgacga cggcgacgag caggaggtgc gccgggcgta ccggatcggc
     4681 gcgctggtcg ggctcctcgc gttcgtcgtg gtgctcatgg gtggccaggg gacgctgctc
     4741 ccctgggtcc ccgccgggga cttctacgac gcccaggccg agtcgttcct ggatggtcgc
     4801 ctccaggtcg accgcgacgt gctcggcatc gaggccttcg agcacgacgg cggcacctac
     4861 atgtaccaac ccccctggcc ggccgtgctg cggctcccgg tggtggccct gaccgatcgc
     4921 ttcgacggac gcctgggcgc gccgtcgatg ctgctcgccc acgtcgtgct gctcgtcgcc
     4981 gccgagcggg tgctgctcga ggcacgccgg gcgatcccgg gtgccgcggc tggggggcgg
     5041 ctcgggcgcg ccctctgcgc cctcttcggc ctcaccctgg ccggcgggtc gacggcgacg
     5101 ttcctcgcca gccggccgtg ggtgtaccac gagtcggcca tgtgggggat ggcgtggacg
     5161 gtggtgacgt tcgccgggct gctcgtcctg cggcgtcggc cctcctgggg cgcgctcgcc
     5221 gtcaccgcgc tcggttgcgg cggtgcggtc atgtcacgag cgtcggtcgg cgccggagcc
     5281 tgcgtcgccg tcggcgtcct cgccctggcc gccctcgccg caccggggcg tcgcgtgctc
     5341 gatcggctgc gcggtcccgc aggcgccgtg cccctgctgg ccgtcgccgc cgtgcccgcc
     5401 gcggtgtacg cagcgctcaa caccctgaag ttcggcaacc cgacgtccat cccgttcgag
     5461 gcccaggggt acacgcaggt gagcgcggca cgccgggaga tgctggcgga agcgggtggc
     5521 agcctgttcg gcctgcggtt cgcgccgacc acgctgtggc actacctccg accgaccggc
     5581 atccgggtcg tcgaccagct cccgttcgtg gacttccccc cggcgggcgg cccggtcgtc
     5641 ggtgacgtca ccttcgacct ggtcgaccac acgtccagca tcaccgtcac cctcgtcgcc
     5701 ctcgtggtcg ccgccgcggt gggggcctgg gcgctggtcc gccgtggacc gaccgaccgg
     5761 gcggcgtggt tcgccgtcgc cctcgggggg ctggcgggat gcgccacgat cgtgcccttc
     5821 gggtacgtgg cgcagcgcta ccacgccgac gtgatcccgt tcctcgtgcc catggccgcc
     5881 ctcggcgtgc acgccgtcag ccgcaccccc gggcggaccc ggcgccggac gtccggcatc
     5941 gtcggcatcg gcctggtcct ggtcaccctg tggttccaga tcgggctcgc caccgtctac
     6001 cagcggctcc gtgccccgaa cccggcgccg gcgcaggtgg ccggcctgct cgaggcccgc
     6061 ctcgacatcg gtgcggaccc cgacgtccgc gtggccgaga ccctcccgga cgccgcgccg
     6121 cgcgatgcgg tggtcgtgat cggcgactgc cgaggcgtct acgtctggag cgcggcgccg
     6181 ccgtcggaca tctacccgcc cggttgggtc gccgccgagc gcacgcgcgc gggcggccgg
     6241 gtcgagctgg ccgtcgatct gtcacagctc gagcccggtc ggcggcacgc cctcgtgcac
     6301 tggaccgagg ccccggtcgt cgcgtgggtc acgccggagg acggccaggt ggtcgtcggc
     6361 atcaccgccg gcgacctcga gtacccctcc cgctcggtgg acctgggcga tggcaccgtc
     6421 gagctcgagg tctacctcga ccccgccctc ggtgaaggtg gcgtccgctg gcggggtcgc
     6481 ctgctcacga acggcttcct cccccgtctc gacggtcccg ccaccctgcg gatcggcgaa
     6541 ccgccggtgg gctcggacta cgccccttcg gcgacgagcg aggtcgggtc gtcggacggc
     6601 gcgctctgcg cacggctggt cgacgccgcg gcggagtgac ccgccggctc cagctcgaag
     6661 aggaactgga aggcgaacag cgtgggccgc acgcgtgcca gcgcccggtc gatcgcaccg
     6721 atcgcagaca ggaccgggcc gacgacgccc gcaccgcccc gctggaagac ctcgacgggg
     6781 aggccggcgg cgtcgaggtc ggcgacccgg tagccggcgt cctcgcacag tcggcggaac
     6841 gagcgtcggg tgtagaagcg caggtggcct cggtcgagga tcccccgcgc gtcgtagtcg
     6901 aacaggcccg acgccacccg cacccgcggg taccagtggg cgaagttcgg aatgctgccc
     6961 acgacgcgcc cgccggggcg gagtcggccg tggacctcct cgagcaacga ggccgggtcg
     7021 cggaggtgct cgagcacgtc ggcgacgagc acgacgtcga agtccccggt cacctcgtcg
     7081 ggcagtccgg cgtcgaggtc ggccacgacg tggcggtcca gccgccgcgc cgcctccggg
     7141 gaggcctcgc cgtcgacgcc gatgacctcg tggccacgct cgcggagcgc gccgccgagg
     7201 tggccgtcgg cgcagccgag gtcgagcacg cgacctgcgg ggtggccgga cagccgctcc
     7261 agcagcagct ggtgcgacgt gccggcaccg accttggcct cgtacccgac gccgcgggtg
     7321 cccgagccga acccgatgcg tcgcagccgg tggcgcagga cgtgccccgt gacctggcgg
     7381 gcgtaccgca tgccgttcac gtaggagatc tcgtcgccgt agtaggtcgg gatcgggatc
     7441 tcgacgatgg agcgaccggc acccatcagg ccgaggagga tctgggtgtc gaagtcgaac
     7501 ccgtccgaca tggtgtcgag gtcgaggtcg cggagcgcgt cgacgcggta cgcccggtag
     7561 cccgagtgcc actccgacag ggacgcgccg gtgagccggt tctgcacggt ggtgaggatc
     7621 cggttgccga cccacttgta gagcggcatc cccccggccc gggccgaccc gggtgggagc
     7681 atccgtgagc ccatcaccgc gtcggcgcca ccgtcgacga tcggagcgac catgtcgggc
     7741 agcagctccg gcgcgtactg gccgtcgccg tggagcagga cgacgacgtc gaggccgtgc
     7801 tccatcgccc accggtagcc ccacttctgg ttcccgccgt agccgcggtt ctcgtcgtgg
     7861 cgcaccaccg tgatcggcag gtgggcgaag gtctcccggt agccgacccc gatcaggaac
     7921 gtgctgtcgg tcgacgcatc gtcggcgacc atgacgtggt cgatcgactc gcggtacccc
     7981 tccgggatgc ggtcgagcac cgacgcgagc gtggacgccg cgttgtaggc gacgacgagc
     8041 acgccgaccc ggggggaggg gacctctgcc acgacggcat cctaggagtc gccgctcgac
     8101 acccccgggt tgggatcggc agcgcgcacg gcggcgcgga gctcatcggc caaccacggc
     8161 cacaccgcct gcgccccgcc acgctcgagg tggatgccgt cggggcgcag caccacgccg
     8221 tccagctcgt ggaccgccac gccgtccggg cacagccggc cggcgagatc cacgaccgtg
     8281 ccgccgaggc gttcgacctc ctcgatcagg atctcgttgt acgccgcgag gcgggcgtcg
     8341 agatcctcgg caccgagcgc ggcgcgggcc gagccggcgg agaagcaggg cgtggacagc
     8401 gcgaccacgt ggtgctccgc agcgagtccc gccagcgtcg cctcgacgtt cgctcggagg
     8461 cggtccgccc aacgtgggtc ctcgaacccg atccgtccgg cgtccgtggc ctggtcgagc
     8521 acctcccaga cgccggcgtg cacgaccacg gcgtcgggct cgagcgccgc caggtcgtcg
     8581 gggagcgcag cgagccccgg accgcaccct gccttgtcga tgtcctgacc gcccatcacg
     8641 accgactggg tcgacagacc gcagccgagg gcgccgaggt tcccgacgcg caggtcgagc
     8701 tcgtcgaacg gcggggcgtg ctcgaccagg cggagcgctt gggagtcgcc gaccacgacg
     8761 acgaacggga gctggtcgag gcccgtgagc cgggagagcc cgtcgaggat ccaccgtgcc
     8821 gtcgaccgcg cacccgccgg ctccatgcgc gccaggtcga gcagctccga cgggaagccc
     8881 cgggtcacct cgtcggagat ccggtcgagg cagccggatg acttgtccac ggtgccgtct
     8941 gcggtcggtg cggtggtggc caggaccacg accgcgccgg acgagccgag cacgtcgacg
     9001 acgtcgcgga gctcctcccg gtggtgggcg tcgtacatcg ggtcgcacgg ccctcgcagg
     9061 acgccgtcac cgaggtcccg gtcgtccgtc ccggcccagc ccgcgaggag cagcaccacg
     9121 tccggctgct cgcgcgcgac gaccgacgac caccgctgcg accagcccgt gcagtccacg
     9181 cacccgatct cgccttcgac ccggacccgc agctgctggg cctccccggc cgacgcgacc
     9241 tcccgggcga gcgtgaccgt tcccgggttg ccgatcacga ggagcctccc ggcgaccgcg
     9301 gtcgcctcgg ttcccgcagc accgaccgcc aggtcggatg tccgctcctc cgcggacgga
     9361 ccggcctgcc agacgaggac gaccacgagc gcggcggtgg ccgcgaccgc cccggcggag
     9421 acgagcgcgg ggcgcgggat gcgccagcgc tggctccgga tcggcgcctc gagcaggtgg
     9481 tacgacgccg tggcgatggc gatggacacg gccaggcgca gcccggtcag cgaccacccc
     9541 tcgaggccgg tgcggtccgg gtcgagcacg acgtacacgg gccagtgcca caggtacagc
     9601 ccgtagctga ggatgccgat ccggacgagg ggtcggacgc gcagcacctg gccgagccgt
     9661 ccgccgaagg ggccggacac ggcagcgatc acgaccgcgg tgagcacggc gtgggcgagc
     9721 aacccgtacc ggtacagcca cagctcggtc ccgtccacca ggacccagct ggcgagcagg
     9781 gcaccgaggg acgcgacggc ggccgcagcg accccgcgct caacgccgga cggccgccgg
     9841 gacgccccgg ccgtgatcgt ggcgagggcc gcgccgacca ggatcgacgc ggcccgggtg
     9901 tcggtgccgt agtacgcccg ggtgaccccg ccggccccct cgacgacgac ccaggaaacg
     9961 accagcgacg cgaccagacc ggcgccgacc agggcacgga tgccgacgcg ccggccgagg
    10021 agacgcacgc tgccgagcac cagcagcggc cacacgacgt agaactgctc ctcgatcgcc
    10081 aggctccaga cgtgctcgag gggcgtgggc gcgtcgaaca gcgcccagta gtcgcggctg
    10141 ctcgcgatct cgccccagtt ggcgacgtag gcgagggtga gcagggcctg gttgccgaag
    10201 cgctccatct cggcgctggg cagtgtgagc gtcgccacca ccgtggcggc cgcgaccacg
    10261 gcgagcatgg cggggagcag ccggcgggcg cgcctcgccc agaaggcgcg cagcgagatc
    10321 cggccgcgct cgcggtgctc caccaccagg agcgacgtga tcaggaagcc ggacagggtg
    10381 aagaacaggt cgacgccgag gaacccgccg gccaggtggt cggcgtggaa caggagcacc
    10441 ccgatgaccg ccacgccgcg gagcccatcg agcgcctcga cgtgcgggat cgtggggcgc
    10501 gcacgggcgc tcgaggtcga ggtggctacc acggtggcgg acgaacctag cgagcgccct
    10561 gcacgtcgac gcagcgcccc gacggtcccg catcgcaccc tccgccgtcg ggtagagcgg
    10621 agcacatgac ccagacggcc cccaacgacc tcgcggtcac ccacgacgag gacgccaacc
    10681 gctacctcct gcgtcggggg gacgacctcg tcggcacgct cgcctaccga agcgtcggac
    10741 gacccgacga ggagacgatc aacgtgttct ccaccaacat ctcccccgcc gtgcgcggcc
    10801 agggcctcgg cgaggtcctg gtgcgaggtg cgctcgacga cctgcgcacc cgcggcacca
    10861 gcgtcatggc ctcgtgctgg tacgtcgccg acttcctcga cgccaacccc gactaccagg
    10921 acctgcgccc cgaggcctga tcagctgctg cggcccaccg gcacccggcg gcgcacgccc
    10981 caccacgtca cgagcacgag ggcctcgacc acgatgcgcc ccgacatctt cgaggtgccc
    11041 cgcgtgcggt cggtgaacga gatcggcacc tcgtcgatgc ggccgccgtt gcgggccacc
    11101 cggtcggcca tctcgatctg gaacccgtag ccgtcggccc gcaccgagtc gaggtcgatg
    11161 tcggccagcg ccgaggcccg gtaggcccgg tagccggcgg tggcgtcggc cacgtcgagc
    11221 gacagcacgg caccggcgta gcggttcccc cagcgcgaca gggccttgcg gtgccacttc
    11281 cagtccggga tcgacccgcc cggcacgtag cgcgatccga tggccaggtc ggcgccgtcc
    11341 tcgacccggg cgagcaggct cggcagcgcg gccgggtcgt gggacagatc ggcgtccatc
    11401 tccaccatga cctcgaagcc ctcgtcggca ccccaggcga agccggcccg gtaggcggat
    11461 ccgagcccgg ccttgccggc gcggcgcagc aggtggagct cggcgtcgtc ggtggccatg
    11521 gcctcgacga ggtcgccggt gccgtccggg ctgttgtcgt ccacgaccag cacgtgcgca
    11581 tccgggacgg cagcatggac ccggcccacc acgtcggcga tgttctcggc ctcgtcgtag
    11641 gtcgggatga tgaccaggac gcgcatggtc agtagggcca ggtctcgccg tcgtggccca
    11701 gcttgcgggc ccagtcgtgg cggccggtga ccatcaggac cttgatcgcc acgttccggg
    11761 cgaaccacgg cagctgggcg agctggcgga gcgccgaggt ggcgcgtgcg gcaggcgact
    11821 gggcggtgcg gcggtaggag gtgagcgagc cggcccgacc gtcgtagcgc cagtcgggcg
    11881 agaccagcag ctcgacgagg atcgccaggg tcacgccgat cgaggagaac gagtcatgga
    11941 tcagcatccg cccgccgggc gccacccggg cgccccagtc gcggatgtcg gcccgggccg
    12001 ggccgaagcg gtgggccccg tcgatgaaca gcacgtcgat cgggtcggcg acgtcgccgt
    12061 gggccgcatc ggagaaggcc cggacgtggc gcacccgctc ggcgacgccg gcgtcggaca
    12121 ggttgcggtt gaagacctcg tggtcggtgg ccgcctcggc ttcgaagccc tcgatctcct
    12181 ggggtccgcg gtcgttgccg gcgtgggggt cgatggccac gatctcgacg tcggcggggg
    12241 ccgagctggc gaggaccacc gtggaccggc cgcggaacga gccgatctcc acgatccgcc
    12301 cgccgggagg gcaggccagg gcggcctcgt gcagcgcctg gatctgggcg tcggtgagcc
    12361 agccctcgac gtcgatgacg cgcgcctgca cctcggcgag gctgggaggg gatccggggt
    12421 cgctggcgtc gttcacggcg ctgcagcgta ggggtggggc tgccccggag ccgtgatcgg
    12481 cggatgccgg gggccgcccg ggcggatcgg tagggtcgcg gtcccctggc accggtccgc
    12541 gtggtgccgt ccgtggagtc cgcacgtgcc cgaacacccc accaccgacg ccaagatcgg
    12601 cgacctcgcc gcggccgccg gcatccgtcg gatccacgtg ctggcctggc gcgacctcga
    12661 cgacgtcgag gcgggcggct ccgagctcca cgcccacgag gtgtgccggg agtgggcggc
    12721 cgccgggctc gaggtgacgt gccgcacgtc gttcgcccag gggcacccgc ccgagatcat
    12781 gcgcgacggc taccgggtgc tgcgcaaggc cggccgctac ctggtgttcc cccgctcggt
    12841 ggccgccgag ctggccggcc gcaacggcga gcgggacgcg ctggtggaga tctggaacga
    12901 catgccgttc ctgtccccca tctgggcccg gggcccgcgc atcaccttcc tccaccacgt
    12961 ccacggcgag atgtggaaga tggtgctccc gccccgcctg gcgaagatgg gcgacctgct
    13021 cgagcgcagg ctcgcacccc cgctgtaccg gcacagccgc atcgtgaccc tgtcggagtc
    13081 ctcccgcgac gacatgctcg cccagctccg cccccgcccc gagcgcgtcg acgtggtccc
    13141 gcccggcatc gatccgaagt tcagccccgg tgaggagtcg ccgacgccgc tggtggtggc
    13201 ggtgggccgg ctggtgccgt ccaagcacgt cgatcgcctg gtgcgggccg tggcccacgc
    13261 ccgccgatcc gtgcccgacc tgcgcctggt cgtggtcggc gagggctacg agcggctcca
    13321 cgtcgaggcc acggtcgaag acgtcggcgg cgaggcctgg gtcgacctgc ccggccgggt
    13381 caccgacgac gagctgatcg agctgtaccg ccgcgcctgg gtgctggcct cggcgtcgtc
    13441 ccacgaggga tggggcatgt ccatcaccga ggcggccgcg tgcgcgaccc ccgccgtggt
    13501 caccgacatc cccgggcacc gcgacgccgt ggtcgacggg gtcaccggca cgctggtcga
    13561 cgccgacggg gacgccctgc ccgaacgcct cggcgacgcc atggccgccg tgctcgccga
    13621 cccggagcgg cgtcgtcgca tgtccgacgc cgccgccggc cacgcggccg ggttcacctg
    13681 gcggtcgacg gcgacgcagc tgatggccat cctcgccgac gaggccgggc gtccgcgccg
    13741 ctcgtgaccg ccccccgcac cccgcgggcg cgggactggt ggctgccgct ggccgtgctg
    13801 gccgtgctgg tctacgtccc cctcctgctc accgatcccg gccacgtcat cgccgacacc
    13861 aagagctacc tgtacctcga ccccggccgc ctgctgtccc gggcggtgtc gatgtgggat
    13921 ccccacgtcg gcctcggcac cgtcccccac cagcagatcg gctacctctg gccggccgga
    13981 ccctggtact gggtcttcga acagctcggc atccccgact gggtcgccca gcggctctgg
    14041 ctgggcacga tcatggtcgg cgccggcgga ggcgtgctgt tcctcggccg cacgtggcgc
    14101 tggcgcccca cctcggccac cgcggcggcg ttcctctacg ccctgagccc gttcgtgctg
    14161 acgctggcgg cgcgcatctc ggtgctgctg ctgccgttcg ccggcctgcc ctggctgctc
    14221 gccctcaccg tgcgggccct gcactcgaag ggctggcggc acccggccct gttcggcctg
    14281 atcgtggtca ccgtcggcag cgtgaacgcc acgtcggtcc tgctcgtcgg cctcgtcccg
    14341 gtgctgtgga tcctgtactc ggccttcggc tcccgcgacg tgtccaccag ccgcgccgcc
    14401 accaccacgg ccaagatcgg tgtcgtcacc ctcgccgtca acctgtggtg gatcagcggc
    14461 ctctcggtcc aggccaccga cggcatcgag gtcctgcgct acaccgagac ggccaaggtc
    14521 gtcgccgatg cgtcggtgtc gcacgaggtc ctgcgcggcc tcgggtactg gttcttctac
    14581 ggcggtgacc gcctggggcc gtggatcgag ccgagcgtgg actacaccca gcggctctgg
    14641 ctcctcgggc tgacgtacgc gctcccgatc ctgggcctgc tcgcgctcgg cgtggtgcgc
    14701 tggcggcacc ggacgttcct cgtcggcatg ctcctgctgg gcacggtcat cgccatcggc
    14761 gcacacccct ggggctcgcc ctctcccatc ggcgggttca tcaaggcgtt cctgctcacc
    14821 gacgtcggcc tcgccatgcg cagcctgccc cgagccgtcc ccctggtcgc cctggccctg
    14881 gccctggcca tcggcgcggc cgtgggcgcg ctggccgagc aggtcacccg gcgcggcatc
    14941 atcggcgccg tcgcgatcgc cggcctcgcc tacgccgccc tcccaccgct gtggctgcgc
    15001 gagatggcgc cggtgaacct gcagcgcccc gaggacatcc cgtcgtactg gcacgacgcc
    15061 gcgtccaccc tcgacgagcg cgacgacggg acccgggtcc tcgagatccc cggcatcgac
    15121 ttcgcctcct accgctgggg caacaccgtc gaccccgtcc tccccggcct gatggaccgc
    15181 ccgtacgcgg cccgggagct catcccctac ggcacgcccc cctcagcgga cctgctgaac
    15241 gccctggacc tggagctgca ggagcagaca atgtcggacc agggcctggc ggccgtggcg
    15301 cggctcatgg gcgtcggcga cgtcgtgctg cgcaacgaca cccagtacga gcgttacaac
    15361 accccccgac cccggtccct gctcgacctc gtgtcgtcga tgcccggctt cggcgacccg
    15421 atcacgttcg gcgacgacgt ccccaacgtg ccgatcgagg acgcgccgat gagcgacgag
    15481 cagcccctgg cccgcgacgg cgctcttccc gagtcgcccg ccatcgtgac gtt
//
LOCUS       DATISU010000458         3469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_185163, whole genome shotgun sequence.
ACCESSION   DATISU010000458 DATISU010000000
VERSION     DATISU010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185163"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VLR27_18195"
     CDS             <1..1186
                     /locus_tag="VLR27_18195"
                     /inference="COORDINATES: protein motif:HMM:NF024622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HSP05441.1"
                     /translation="LDLPWQLPLLEWDVDNTDGRLVRMAHGVSRHVVRFVQYEGRVYA
                     LKSTEESVAHREYGLLRGLAEARLPVVEAIGVVTGRATPDGEPLGAVLVTRYLDFALP
                     YRYLFGNPTGMDLRPRLVDAGVVLLARLHLEGFFWGDCSLNNILFRRDAGALMAYLVD
                     AETGEHHDSISDSMREHDLEIARINIAGDLTDLIASGRIPEIDVVAAVDDVVARYREL
                     WEELTRSEEFPSSERHLIESRIRRLEDLGFDVSEVSLRPTQGGSTIRMRPVLLEEGHH
                     ARELERLTGLRVQENQARRFLADITSWQAYLSQEEGRDVPRAIAAARWLEEVYAPHTV
                     VPPELADRLEPAELFHELLEHRWFLSEQRGGHVSNDEALEDYVSSVLGTRPPERIVVE
                     DD"
     gene            complement(1126..1350)
                     /locus_tag="VLR27_18200"
     CDS             complement(1126..1350)
                     /locus_tag="VLR27_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05442.1"
                     /translation="MTISPLPLLDVTDAGTTWPDDVADLTDRRRLLVRAVLGVDLPRR
                     SVDQVFCLDGAQSSSTTMRSGGRVPSTDDT"
     gene            1476..1754
                     /locus_tag="VLR27_18205"
     CDS             1476..1754
                     /locus_tag="VLR27_18205"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HSP05443.1"
                     /translation="MEDELEVLRRQIDAVDERFVELLAERFRITRRVGHIKSDRGLPA
                     QDSIREAQIDEKVRRLAAEHALDEGLVSDVLRAVIDRVVAEHRELHSR"
     gene            1751..2389
                     /locus_tag="VLR27_18210"
     CDS             1751..2389
                     /locus_tag="VLR27_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05444.1"
                     /translation="MTEPVTSATDIAAPAGRPTSISDRLPAPPAAGHRTRAELDAQLD
                     HLRAAPVDHGTLELVVCRPALLQRRVLEVGTLSCTEGLVGDTWSQRPSKRTPDGSPHP
                     DMQLNIMGSRVARLVAVTDDRMPLAGDQLYVDLDLSEAALPAGTRLAIGTAVVEITDQ
                     PHTGCAKFTERFGLDALRFVNSPIGKELRLRGANAKVVVDGEVRPGDRVRRV"
     gene            2423..3328
                     /locus_tag="VLR27_18215"
     CDS             2423..3328
                     /locus_tag="VLR27_18215"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HSP05445.1"
                     /translation="MAPRPHLVLANDDAGSTSRRDVALAVSRLAEHAPTELRWTSTPE
                     EFERLVREASEQQLVIAGGDGSLHLALQTLDALDRTDEAIGIIPLGTGNDFARNHGIP
                     LDPQEAADVVVGGQPRAVDAIELRDDGTHRELVANNLHLGMGVDSARAAQRFKPVLKR
                     FAYPAATAWTGATGGSAPLRVTVEDEVVWDGPLLACLFLLGPSMGGGVELVDTNEGTI
                     DVVVIEPVEPRDRVALVRAAMRDGLSAADEAHRWTGRRASVQAAGVLDTDIDGELCEH
                     DRTLHLELKPRAWKVLVPQGGSTTG"
BASE COUNT          495 a         1294 c         1205 g          475 t
ORIGIN      
        1 cctcgatctg ccctggcagc tgccgctgct cgagtgggac gtcgacaaca ccgacggccg
       61 gctcgtgcgc atggcgcacg gcgtgtcgcg acacgtcgtg cgcttcgtgc agtacgaggg
      121 gcgcgtctac gcgctgaagt cgaccgagga gtccgtcgcc caccgcgagt acgggctgct
      181 gcgcggtctg gccgaggccc ggctgcccgt ggtcgaggcc atcggcgtcg tcaccgggcg
      241 cgccaccccc gacggcgaac ccctcggtgc cgtcctcgtc acgcggtacc tggacttcgc
      301 cctgccgtac cgctacctgt tcggcaaccc caccgggatg gacctgcggc cccgcctggt
      361 agacgccggc gtcgtgctgc tggcccgcct gcacctggag ggcttcttct ggggcgactg
      421 ctcgctgaac aacatcctct tccggcgcga cgccggcgcc ctgatggcgt acctggtcga
      481 tgccgagacc ggtgagcacc acgactcgat ctccgattcg atgcgcgagc acgacctgga
      541 gatcgcccgc atcaacatcg ccggcgacct caccgacctg atcgcatcgg gccggatccc
      601 ggagatcgac gtggtcgccg cggtcgacga cgtcgtggcg cggtaccggg agctgtggga
      661 ggagctcacc cgctccgagg agttcccgtc ctccgagcgg cacctgatcg agagccgcat
      721 ccgccgcctc gaggacctcg ggttcgacgt gtcggaggtg tcgctgcggc cgacgcaggg
      781 cggctcgacc atccgcatgc gcccggtgct gctggaggag ggccaccacg cccgggagct
      841 ggagcgcctc accgggctgc gggtgcagga gaaccaggcc cggcgcttct tggccgacat
      901 cacctcgtgg caggcctacc tgagccagga ggaggggcgc gacgtgccgc gggccatcgc
      961 ggcggcgcgc tggctcgagg aggtgtacgc cccccacacc gtggtgcccc cggagctggc
     1021 cgaccggctg gagccggccg agctgttcca cgagctgctc gagcaccgct ggttcctgtc
     1081 ggagcagcgg ggcgggcacg tcagcaacga cgaggccctg gaggactacg tgtcgtcggt
     1141 gctcggcacc cggccgccgg agcgcatcgt cgtcgaggac gactgagcgc cgtcgaggca
     1201 gaagacctgg tcgaccgatc ggcggggcag gtccacgccg aggacggcgc gcacgagcag
     1261 gcgacggcga tcggtcagat cagcgacgtc gtcgggccag gtcgtgccgg cgtcggtgac
     1321 gtcgaggagg ggaagcggag agatcgtcac ggcatggtcc ttctggccta ggtgtcggca
     1381 ccccataccc cgatcttgac gaagaattcc gacatcgttg atgccagatc ctgaacgggt
     1441 cgtacgcagg ccggtgcatg ggccacgatg gagcgatgga agacgagctc gaggtgctcc
     1501 gccgccagat cgatgccgtc gacgagcgct tcgtcgagct cctcgccgag cggttccgca
     1561 tcacccggcg ggtcggccac atcaagtcgg atcgaggcct gccggcccag gactcaatcc
     1621 gcgaggccca gatcgacgag aaggtccgcc gcctcgccgc cgagcacgcc ctggacgagg
     1681 gcctcgtctc cgacgtgctg cgcgccgtca tcgaccgcgt cgtggccgag caccgggagc
     1741 tgcactcgcg atgaccgaac ccgtcacctc cgccaccgac attgcggcgc ccgccggtcg
     1801 gccgacgtcc atcagcgacc gcctgcccgc gcctcctgct gccggccacc gcacccgcgc
     1861 cgagctcgac gcccagctcg accacctgcg ggccgccccg gtcgaccacg gcaccctcga
     1921 gctcgtcgtg tgccgtccgg ccctcctgca gcgccgggtg ctcgaggtcg gcaccctctc
     1981 ctgcaccgag ggcctcgtcg gcgacacctg gtcgcagcgt cccagcaagc ggacgccgga
     2041 cggcagcccg cacccggaca tgcagctgaa catcatgggc agccgcgtcg cccgcctcgt
     2101 cgccgtgacc gacgaccgga tgccgctcgc cggcgaccag ctctacgtcg acctcgacct
     2161 gtccgaggcg gcactgccgg ctggcacccg cctggccatc ggcacggccg tggtcgagat
     2221 caccgaccag ccccacaccg gttgcgccaa gttcaccgag cgcttcgggc tcgacgcgct
     2281 gcgcttcgtc aactccccga tcggcaagga gctgcgcctg cggggcgcga acgccaaggt
     2341 cgtcgtcgac ggcgaggtcc gccccggcga ccgcgtccgt cgggtctgac tgggcgggaa
     2401 cgcccggggg tatcggcagc ggatggcccc acgtccccac ctcgtcctcg ccaacgacga
     2461 cgccggcagc acgtcacgtc gtgacgtcgc gctcgccgtc agccgcctcg ccgagcacgc
     2521 ccccaccgag ctgcgttgga ccagcacgcc cgaggagttc gagcggctcg tgcgcgaggc
     2581 cagcgagcag cagctcgtga tcgccggcgg ggacggatcg ctccacctcg ccctgcagac
     2641 cctggatgcg ctcgaccgca ccgacgaggc catcggcatc atccccctcg gcaccggcaa
     2701 cgacttcgcc cgcaaccacg gcatcccgct cgacccgcag gaggccgccg acgtggtcgt
     2761 cgggggccag ccccgggccg tcgatgccat cgagctgcgc gacgacggca cccaccgcga
     2821 gctggtggcg aacaacctgc acctcggcat gggcgtggac tccgcccggg cggcccagcg
     2881 gttcaagccg gtgctgaagc gcttcgccta cccggccgcc accgcgtgga ccggtgccac
     2941 cgggggctcg gcgccgctgc gggtcaccgt cgaggacgag gtggtctggg acggcccgct
     3001 gctggcttgc ctgttcctgc tcgggccgag catgggcggg ggcgtggagc tggtcgacac
     3061 caacgagggc accatcgacg tcgtcgtgat cgaaccggtc gagccccgtg accgggtcgc
     3121 cctggtccgg gccgcgatgc gcgacgggct cagcgccgcc gacgaagccc atcgctggac
     3181 cgggcgacgg gccagcgtgc aggccgccgg cgtgctcgac accgacatcg acggcgagct
     3241 gtgcgagcac gaccgcacgc tccacctgga gctgaagccc cgggcgtgga aggtgctggt
     3301 gccccagggc gggtcgacca cgggctagag cagcccgtcg atcaccttca cggccttctc
     3361 ctcgatccgc tggaggaggc ctcgcttctc ccactcgtcg aggtcgatgg gctcggcgcg
     3421 ctgcaggtcc tcgtccatgt gcccgtcgag caccgcggtc atggcctcg
//
LOCUS       DATISU010000459        11769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_510548, whole genome shotgun sequence.
ACCESSION   DATISU010000459 DATISU010000000
VERSION     DATISU010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 11769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11769
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510548"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VLR27_18220"
     CDS             complement(<1..438)
                     /locus_tag="VLR27_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05446.1"
                     /translation="MRISIEQWAPDYGSPMGPDALEETQAAVDAECEVPVDEWAPRSP
                     ASGTSVPDEIAFVDGVRRVEARVWIESDRGSIHQGICASYAAGVVRCNGAATVVDAQV
                     RRSVFSDAPDLVPLVTRHGRFGVCPVTDSAGDALSLALQGAMAD"
     gene            complement(439..2817)
                     /locus_tag="VLR27_18225"
     CDS             complement(439..2817)
                     /locus_tag="VLR27_18225"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HSP05447.1"
                     /translation="MRPVRVELEGFSAFRETTVVDFTDADLFAFVGPTGAGKSSVIDG
                     MIFALYGSVPRYGREGLVHPVISQGRPEARVRLDFEVHGVAHTAVRIVRRTKTGASTK
                     EARLERGGEVLASDAPSVTAAVGDLLGLDLEQFTKCVVLPQGAFAALLHDTTSKRQDL
                     LVKLLDLGVYEQVAQAARRRVQAADHRIAVIDEHLVDLAHATDDAVAAARARVTEVAT
                     VVDRLDAAAPELAALLEKVRSTDDQAADLRRQSELLGGIRVPSDVASVSSRRAEAERS
                     VSAATDAEDAAVRALDEVRTARSDLGDPAAVQAQLDARASLESLAQRVATGVQMVADR
                     EGAAAPLAEGLRVAQEEAEAAERVLASARVEHAAVDLARHLHPGDDCPVCGGQVRELP
                     AHEAGALDEAEARHRAAVASARHAEKAWQDADRELTALRAKLEERRTELAEAEAATAD
                     APPPEDLRTALAAIAEADAAVAQAQQADRAARQRAAEARRALDEATRAEAAARADLLA
                     TRDRVAAYGPPPTGDDLAADWSALATWAEQQSSEAKRRLADLDGERTRLQHAHDELVG
                     QLVSSATAAGIAVPDRSRLRDACVAAAADARTAVERLDADRQRAAGHRTEREAVADQR
                     AVHELLASELGPRRFEAWLLEEALASLVAGASERLEQLSSGRYAMAIDDKKQFEVIDH
                     ANADERRLARTLSGGETFLASLALALALAERVTELSGSGRASLDAIFLDEGFGTLDPD
                     TLDVVASAIEELGASGRMVGVVSHVRELADRVPVRFEVRRGIESSTVERVDL"
     gene            complement(2814..3950)
                     /locus_tag="VLR27_18230"
     CDS             complement(2814..3950)
                     /locus_tag="VLR27_18230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HSP05448.1"
                     /translation="MRLLHTSDWHVGKLMRGASRADEHRAVLAEMAQVAADESVDLVL
                     VAGDVFETSAPGPESEQVVYRGLLDLAEVAPVLVVSGNHDNARRLEAVEPLLSLGRVQ
                     VRTQVARPDEGGVIRLDAGGVPVQVAMLPFVSKRGIVRADALMRDDAYQLSQAYTDRL
                     GVLVRALTDGFAADTVNLVCAHAFAAGGTTGGGERSVHTIEDYSLSALAFPVTAQYVA
                     LGHLHRAQDVPGATRIRYSGSPLQLDFGETANTSSVTVVDVEPGVPAAVHEVPLEAGR
                     KLRTLRGSLAELEPLAGTTDDDFLRIIVTDRRRAGLADEVRALFPHCVDVLVQSPEDT
                     ATPTHAPVAGRSPGELFRSYLDVADLDDPRLPVAFDELLEEVGS"
     gene            complement(3953..5683)
                     /locus_tag="VLR27_18235"
     CDS             complement(3953..5683)
                     /locus_tag="VLR27_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSP05449.1"
                     /translation="MASDDLDPTTPASPGTVGRVIGTEDATPLEYWVGIADDQFLQLD
                     DVVALDRTLPDGQRVELYGMVEQVRARHEGAQYDSDVFLIEGGLLPAQVSRSAKVITT
                     RVEPEVFVPPLPGTAVRKATGDERGRALHFDDMVQKVPAGLSRDGEPFFIDKEFLDGS
                     RGAHVNISGVSGVATKTTYATFLLYSLFHSGALGAATANTKALIFNVKGEDLLFLDHH
                     NARLDEAQRERYGLLGLQPGPFRSVGIVAPPRPGDANATPQVGSRASGVAPFFWTIAE
                     FCKQRLLPFLFADAEDDRQQYTMVVHNVARRLEEATPTDGGGVVIEGQSITRFRELVE
                     LVDDRLNDDATVADWAGRAIGGGTVGAFVRRLFGAVDHLEHLIRADVRRRSSHPVDLD
                     ANQVTVVDIHNLNDRAKRFVVGVVLRQAFQAKEASGTAEPLQFVVLDELNKYAPREGT
                     SPIKELLLDVAERGRSLGIILIGAQQTASEVERRVVANSAIRVVGRLDSAEATRGEYG
                     WLPVVQRQRSTIIKPGTMIVSQPQLPVPVVVEFPFPAWATRFSETDATTGPSTTPGTT
                     VIPDDPFEGL"
     gene            complement(5743..6285)
                     /locus_tag="VLR27_18240"
     CDS             complement(5743..6285)
                     /locus_tag="VLR27_18240"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSP05450.1"
                     /translation="MAVTIRRATRDDLDALLDLREAVAGEGVWIGAQVPLDREGDRAR
                     HLDTIERQDDGTSGVFLVAELDGVMVGSLSAQARIGIAGLGMQVADGHRGQGIGAALV
                     AEAIEWARSASVHKVELECWPWNRRARALYERFGFVEEGYRRRQYRRKDGALWDSVVM
                     GLVLDEDAPGHDERATEPPT"
     gene            complement(6306..6851)
                     /locus_tag="VLR27_18245"
     CDS             complement(6306..6851)
                     /locus_tag="VLR27_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05451.1"
                     /translation="MVPRSARTPETHHYEGRRASVALGLVCGMVALIGGGLFLAEGPS
                     GPGLVGLAVGLAAIAGWVLLVRRLRARGIRIEPGRLGRVGGGSTTWCDLDAVALATVH
                     RYAVIFGGRQVNLVLWTATGGDRGLAARFARAGMTDEQRQALAAAEASAGTALAPFVV
                     ELSDLPAGASDAVVEHVRHLV"
     gene            6958..7398
                     /locus_tag="VLR27_18250"
     CDS             6958..7398
                     /locus_tag="VLR27_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05452.1"
                     /translation="MEAAQDIQFDFEGSLELARQLWAMAEDAQTVMAGRGRLAQDALN
                     TWLGPHGIDFADRVTSEGRQIDAIAADFRAAANGWASCWQAAMDEQRRIDHARECDRI
                     RNNRSLLDSFVGGIMGHDDLPPPPLPVGLPVAPAFAPTASRWSC"
     gene            7400..>11769
                     /locus_tag="VLR27_18255"
     CDS             7400..>11769
                     /locus_tag="VLR27_18255"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HSP05453.1"
                     /translation="MTTTSADPERLGAFVRGARGARDGLESRQRALTSLYASVHAACG
                     YRRIDCASVGALATLLDGMEANERFVATVRTELLAADSTGSGTVTIDSAVIDNALLAA
                     GVGRAPAAVEFDPAVMVGLPPTSGYVDDPICAANGNMVHQDTDVEFPAIAGALTLTRT
                     WNSLRHRQPGAFGPGWSSVLDVRLAIGDGVVRAHLADGAIVPFVPVDDSADGRRWEVR
                     GRRGLTLTEIDEGFELDVDPVRSFRFDGSGALLGWRSGVAEVTVTRNDHGRVIGLHEQ
                     RTGRSLAVHWAGGYVESVTTDDGRTVSYERTASATIATARSHAGSLRYVWHRETMLRS
                     VVDADGVAAFVNEYDDAARVLRQTSPHGRRTQYHYEAGGSAVITGDDGVQQAMIHDAR
                     GNLTAVVDHDGSVMRIEYDDADRAVRVTERDGAVWHYEYDERDDLVRRVDPDGGTQTW
                     AWDDRHRVVATTDRAGATTRMEYDTDHQAPTRVIGPDGGISTQVLDDRGLPLEITDPD
                     GVVTRFRWDRDGQLVSTTDALGNETRFDHDAHGLLTGLVPPAGAATHLQRGDDGRVQR
                     SIRGDAVASWTYTPAGRITAGVEPGDVPWSATFGAHGDLETFTDGTGATVEMRYDSLG
                     NVVEVVAPDGAVYASTYDAVGRHVSASDPSGATVTKGYDQRGRLVSFTDAEGGTWIRE
                     LDVLGRTVASTAPDGRTTRWMHHPRGEVASVTTPDGRVWTVDVDVAGRPVAVTDPTGA
                     RSEIGYTPAGRLAWRRSPAGRTESFEYDAAGRLVATVDPDGTRRELSLDPLGRVVAVE
                     DAEGRLELSWDDEHHLAEAALPGGGTATYERDAGGRVRRFTDPTGTSTRFEWDERGLP
                     STAFDPAGIPTRFRHDQRGRLQGIDSTGDRTLSLDYGIDGRLAQVSDPTGVTTAYRRD
                     ANGRMTGLRHRDGSGWDRTLDPVGRELSRTSTEGDVTQARAYDAAGRISAATVEDLTT
                     EFLWDDADRLTSVTGVDGGTTRIERDLDGWVVGLVDVDSGERTTFERDGRGRITRVSV
                     DGEGVDVPTRSELPRDLAGRLAIGADGTAYRYDDSGRLAEVLPQDEPATTYAYDDDGL
                     VAVETGPGGIRHFTYDAAGRVVAVRVEGVGTTDIEYDSRGRRVLETRPDRSTVRYGWD
                     ALDRLASIETRRWPDDDEATRLTIRYDALDRPVLVDGDRVDHDPVTGQVRRLGDRRFV
                     PVDSIAYDTATRSWQEPGPRRPLGGTPAGPVWILGARVLDPATHQFLSPDPLLPVAGT
                     NGASSAYTYAWQDPVNHVDPRGLRPVSMEEYDAIRQREEQGRVGQAYQAIREDPWGSL
                     ATVGVVAAGVGLMFVPGGQVVGAGILIGAAAGAGIGFATGNFSPTGVAVSGAFGAIPG
                     GSTMRSALTVGAATGAGETVVTSALTGQGFPSGRELVFGTVTGSAVGGGAHRLTSGLG
                     STVDDLVPARSTAALDHAPTA"
BASE COUNT         1548 a         4294 c         4474 g         1453 t
ORIGIN      
        1 gtcggccatg gcgccctgga gcgcgaggga gagggcgtcg ccggcgctgt cggtgaccgg
       61 gcagacgccg aagcggccgt ggcgggtcac gaggggcacc aggtcgggtg cgtcggagaa
      121 cacgctgcgc cgcacctggg cgtcgaccac ggtggccgca ccgttgcagc ggaccacccc
      181 ggcggcgtag ctcgcgcaga tgccctggtg gatgctgccc cggtcggact cgatccacac
      241 gcgggcctcg acccggcgga cgccgtcgac gaacgcgatc tcgtcgggga cggaggtgcc
      301 ggacgccggc gaccggggcg cccactcgtc caccggcacc tcgcactcgg cgtcgaccgc
      361 ggcctgggtc tcctccaggg cgtcgggtcc catcgggctg ccgtagtccg gcgcccactg
      421 ctcgatcgag atgcgcatct acaggtccac ccgctccacc gttgacgact cgatgccgcg
      481 ccggacctcg aagcgcacgg gcacccggtc ggcgagctcg cgcacgtggg acaccacccc
      541 taccatccgg ccgctggcgc cgagctcctc gatggcgctg gccacgacgt cgagggtgtc
      601 ggggtcgagg gtgccgaagc cctcgtcgag gaagatggcg tcgaggctcg cccgaccgga
      661 gccggagagc tcggtgaccc gctcggcgag ggcgagcgcc agggccaggg aggcgaggaa
      721 cgtctcgccc cccgagaggg tgcgggccag gcggcgctcg tcggcgttgg cgtggtcgat
      781 gacctcgaac tgcttcttgt cgtcgatggc catggcgtag cggcccgacg acagctgctc
      841 gagccgctcg gacgcaccgg ccaccagcga ggccagcgcc tcctcgagga gccaggcctc
      901 gaaccggcgg gggccgagct cgctggcgag cagctcgtgg acggcccgct ggtcggccac
      961 cgcctcgcgc tcggtgcggt ggccggcggc gcgctggcga tcggcgtcga gccgctcgac
     1021 cgcggtgcgg gcatcggcgg cggccgccac gcacgcatcg cggaggcggg accggtcggg
     1081 cacggcgatg cccgctgcgg tggccgagct gacgagctgg ccgaccagct cgtcgtgggc
     1141 gtgctggagc cgggtgcgct cgccgtcgag gtcggccagg cggcgcttcg cctcggacga
     1201 ctgctgctcg gcccaggtgg ccagcgccga ccagtcagcg gcgaggtcgt cgccggtggg
     1261 aggcgggccg taggccgcga cccggtcccg ggtggcgagg aggtcggcgc gggctgctgc
     1321 ttcggcccgc gtggcctcgt cgagggcgcg gcgggcctcc gcagcccgct ggcgggcggc
     1381 ccggtcggcc tgctgcgcct gggcgaccgc agcgtcggct tcggcgatgg cggcgagcgc
     1441 ggtgcgcagg tcctcgggcg ggggcgcgtc cgcagtggcg gcttccgcct cggccagctc
     1501 ggtccggcgc tcctcgagct tcgcccgcag tgcggtgagc tcgcggtcgg cgtcctgcca
     1561 ggccttctcg gcgtggcgag ccgaggcgac cgcggcgcgg tggcgggcct cagcctcgtc
     1621 gagcgcaccg gcctcgtgtg ccggcagctc ccggacctgc ccgccgcaga ccgggcagtc
     1681 gtcgccgggg tgcaggtgcc gggcgaggtc gacggcggcg tgctcgaccc gagcgctcgc
     1741 cagcacgcgc tcggccgcct cggcctcctc ctgcgcgacc cgcagccctt cggcgagggg
     1801 cgcggcggcg ccctctcggt cggcgaccat ctggacaccg gtggcgaccc gctgggcgag
     1861 cgactcgagc gacgcccggg catcgagctg ggcctgcacg gcggccggat cgcccaggtc
     1921 gctgcgagcg gtgcgcacct cgtcgagggc ccggacggcg gcatcctccg cgtccgtcgc
     1981 agcggacacg gaccgctcgg cctcggcccg ccgggacgac accgacgcca cgtcgctcgg
     2041 gacccggatg ccgccgagca gctcggactg gcggcgcagg tcggcggcct ggtcgtcggt
     2101 gctgcggacc ttctcgagga gcgccgcgag ctcgggggcg gcggcgtcga ggcgatcgac
     2161 cacggtggcg acctcggtga cgcgagcccg ggcggccgcg acggcgtcgt cggtggcgtg
     2221 ggcgagatcg accaggtgct cgtcgatgac cgcgatgcgg tggtcggcgg cctgcacgcg
     2281 acgccgggcg gcctgggcga cctgctcgta gacgccgagg tcgaggagct tgacgagcag
     2341 gtcctggcgc ttggaggtgg tgtcgtggag cagggcggcg aaggcgccct ggggaaggac
     2401 gacgcacttg gtgaactgct cgaggtcgag gccgagcagg tcgcccaccg cggcggtgac
     2461 cgagggcgcg tccgaggcca gcacctcgcc gccacgctcg agccgcgcct ccttggtgga
     2521 cgcgcccgtc ttggtgcggc gcacgatgcg gaccgcggtg tgggcgacgc cgtgcacctc
     2581 gaagtcgagc cgcacccgtg cctcgggccg gccctggctg atgaccgggt ggaccaggcc
     2641 ctcccggccg tagcggggca cggacccgta gagggcgaag atcatgccgt cgatgacgct
     2701 ggacttgccg gcgccggtgg gaccgacgaa ggcgaacaga tctgcgtcgg tgaagtcgac
     2761 gaccgtggtc tcgcggaagg cggagaaccc ctcgagctcg acgcggaccg gcctcacgag
     2821 ccgacctcct cgagcagctc gtcgaaggcc acgggaagcc gggggtcgtc gaggtcggcg
     2881 acgtcgaggt agctgcggaa cagctcgccc ggcgaccggc ccgcgaccgg ggcgtgggtg
     2941 ggcgtcgccg tgtcctccgg ggactgcacg agcacgtcga cgcagtgcgg gaacagggcg
     3001 cggacctcgt cggccagccc ggcccggcga cggtcggtca cgatgatgcg gaggaagtcg
     3061 tcgtcggtgg tgccggcgag gggctcgagc tcggcgagcg acccccgcag cgtccgcagc
     3121 ttccgcccgg cctcgagggg gacctcgtgc acggcggcgg gcacaccggg ctcgacgtcg
     3181 acgaccgtga ccgagctggt gttggccgtc tcgccgaagt cgagctgcag cggcgagccg
     3241 ctgtagcgga tgcgggtggc gccggggacg tcctgggccc ggtggaggtg gccgagggcc
     3301 acgtactgcg cggtgaccgg gaaggcgagg gcgctgaggc tgtagtcctc gatggtgtgg
     3361 acggagcgct cgccgccgcc ggtggtgccg ccggcggcga aggcatgggc gcagacgagg
     3421 ttgaccgtgt cggcggcgaa gccgtcggtg agggcgcgga cgagcacgcc gagccggtcg
     3481 gtgtaggcct gggacagctg gtaggcgtcg tcgcgcatga gggcgtcggc gcgcacgatg
     3541 ccccgcttgg agacgaacgg gagcatggcg acctgcaccg ggaccccgcc ggcgtcgagg
     3601 cggatgacgc cgccctcgtc gggtcgggcg acctgggtgc gcacctgcac ccggccgagg
     3661 ctgagcagcg gctcgacggc ctcgaggcgc cgggcgttgt cgtggttgcc ggacacgacg
     3721 agcacggggg cgacctcggc caggtcgagc aggccccggt agacgacctg ctcggactcg
     3781 gggccgggcg ccgaggtctc gaacacgtcg ccggcgacga ggacgaggtc gaccgactcg
     3841 tcggccgcga cctgggccat ctcggccagg acggcgcggt gctcatcggc gcggctggcc
     3901 ccgcgcatca gcttgcccac gtgccagtcg gaggtgtgga ggaggcgcac ggctacagcc
     3961 cctcgaacgg gtcgtcgggg atcacggtgg tgcccggggt ggtggagggt ccggtggtgg
     4021 cgtcggtctc ggagaaccgg gtggcccacg ccgggaacgg gaactcgacg acgacgggca
     4081 cgggcagctg gggctggctc acgatcatgg tgcccggctt gatgatggtg gagcgctggc
     4141 gctgcacgac gggcagccag ccgtactcgc cccgggtggc ctcggccgag tcgagccggc
     4201 ccaccacgcg gatggcggag ttggccacga cccgacgctc gacctcgctg gcggtctgct
     4261 gggcgccgat gaggatgatg ccgagggagc ggccgcgctc ggccacgtcg agcaggagct
     4321 ccttgatggg cgaggtgccc tcgcgtggcg cgtacttgtt gagctcgtcg agcaccacga
     4381 actgcagcgg ttcggccgtg ccggaggcct ccttggcctg gaacgcctgg cgcagcacca
     4441 cgccgacgac gaagcgcttg gcccggtcgt tgaggttgtg gatgtcgacc acggtgacct
     4501 ggttggcgtc gaggtcgacc gggtggctgc tgcggcgccg gacatcggcc cggatgaggt
     4561 gctcgaggtg gtcgaccgcg ccgaacaggc gccggacgaa cgcgccgacc gtgccgccgc
     4621 cgatggcccg gccggcccag tcggccacgg tggcgtcgtc gttgaggcgg tcgtcgacca
     4681 gctcgaccag ctcgcggaag cgggtgatgg actggccctc gatgaccacg ccgccgccgt
     4741 cggtgggcgt ggcctcctcc agccggcggg ccacgttgtg cacgaccatc gtgtactgct
     4801 ggcggtcgtc ctcggcgtcg gcgaacagga acggcagcaa ccgctgcttg cagaactcgg
     4861 cgatggtcca gaagaagggc gccacgccgc tggcccggga gccgacctgg ggggtggcgt
     4921 tggcgtcacc cggccggggc ggggccacga tgccgacgct gcggaacggg ccgggctgga
     4981 ggccgaggag gccgtagcgc tcgcgctggg cctcgtcgag gcgggcgttg tggtggtcga
     5041 ggaagagcaa gtcctcgccc ttgacgttga agatgagcgc cttggtgttg gcggtggcgg
     5101 cgccgagggc gccggagtgg aacagcgagt agagcaggaa ggtggcgtag gtggtcttgg
     5161 tggccacacc ggagacgccc gagatgttca cgtgcgcgcc ccggctgccg tcgaggaact
     5221 ccttgtcgat gaagaacggc tcgccgtcgc gcgagagccc ggccggcacc ttctgcacca
     5281 tgtcgtcgaa gtggagggcc cgacctcgct cgtcgccggt ggccttgcgc accgccgtgc
     5341 ccggcagcgg cggcacgaac acctcgggct cgacgcgggt ggtgatgacc ttggccgacc
     5401 gcgacacctg cgccggaagc aggccgccct cgatgaggaa gacgtcgctg tcgtactggg
     5461 cgccctcgtg gcgggcccgg acctgctcga ccatgccgta cagctccacg cgctggccgt
     5521 cgggcagggt gcggtcgagc gcgaccacgt cgtcgagctg gaggaactgg tcgtccgcga
     5581 tgccgaccca gtactcgaga ggggtggcgt cctcggtgcc gatgacgcgc ccgacggtgc
     5641 cgggcgatgc cggggtcgtg gggtcgaggt cgtcggaggc catggcgatc gacggtaccg
     5701 acggggggtg acaggaacgg ggagccgccc gtcaccccgg gttcaggtcg gcggctcggt
     5761 cgcccgctcg tcgtgacccg gagcgtcctc gtcgagcacc aggcccatga ccaccgagtc
     5821 ccacagggcg ccgtccttgc gccggtactg gcgacgccgg tagccctcct cgacgaaccc
     5881 gaaccgctcg tacagtgccc tggcccggcg gttccacggc cagcactcga gctcgacctt
     5941 gtgcacgctc gcgctgcggg cccactcgat cgcctcggcc accagcgcgg cgccgatgcc
     6001 ctgcccccgg tggccgtcgg cgacctgcat gcccaagccg gcgatgccga tccgcgcctg
     6061 cgcggacagc gacccgacca tgaccccgtc gagctcggcg acgaggaaca cgccgctggt
     6121 cccgtcgtcc tgccgctcga tcgtgtcgag gtgccgggcc cgatcgccct cccggtccag
     6181 cggcacctgc gcaccgatcc agacgccctc cccggccacc gcctcgcgga ggtcgagcag
     6241 ggcgtcgagg tcgtcgcgcg tcgcccggcg gatggtcacg gccatgggga cagtgtgcgg
     6301 cgcggtcaga cgaggtgacg cacgtgttcc acgaccgcgt cggaggcacc ggcggggagg
     6361 tcgctgagct cgacgacgaa gggggcaagc gccgtgccgg cggacgcttc tgccgcggcg
     6421 agggcctggc gctgctcgtc ggtcatgccg gcccgggcga agcgcgccgc cagcccgcgg
     6481 tcaccccccg ttgcggtcca caggacgagg ttgacctggc ggccgccgaa gatgaccgcg
     6541 taccggtgca cggtggcgag cgccacggcg tcgaggtcgc accacgtggt ggagccgccg
     6601 cccacccggc cgagccgacc gggctcgatc cggatcccgc gggcccggag gcggcgcacg
     6661 agcagcaccc agccggcgat cgccgcaagg ccgaccgcga ggccgacgag cccgggaccg
     6721 gagggtccct cggcgaggaa gaggccaccg ccgatcagcg cgaccatgcc gcagacgagg
     6781 cccagggcga cgctcgcccg cctcccctcg tagtggtggg tttcgggcgt tcgggcggat
     6841 cgcggtacca cgggcgcagg ctaggctcgc ccgccgcgca ggtggtcgac ggcacggcag
     6901 gatctcgccc accgttgtcg aagagtggat cggcgatcgt ctggcggtgg aggaacggtg
     6961 gaggcggccc aggacatcca gttcgacttc gagggcagcc tcgagctggc ccgccagctc
     7021 tgggccatgg ccgaagacgc gcagaccgtc atggccggcc gcggccgcct ggcccaggac
     7081 gcgctgaaca cctggctcgg cccccacggc atcgacttcg ccgaccgcgt gacctccgag
     7141 ggtcgccaga tcgatgccat cgccgccgac ttccgcgctg ccgccaacgg gtgggcatcc
     7201 tgctggcagg cggccatgga cgagcagcgc cgcatcgacc acgcccgcga gtgcgaccgc
     7261 atccgcaaca accgcagcct gctcgactcc ttcgtcggcg gcatcatggg ccacgacgac
     7321 ctcccgccac cgccgctgcc ggtcgggctg cccgtcgccc ccgccttcgc gcccaccgcc
     7381 tcgcgctgga gctgctgaga tgaccacgac gtcggccgac ccggagcgcc tcggcgcgtt
     7441 cgtgcgtggc gcccggggtg cccgcgacgg actcgaatcc cgccagcgcg ccctgacctc
     7501 gctgtacgca tcggtgcacg ccgcctgcgg ctaccggcgc atcgactgcg cgtccgtcgg
     7561 cgcgctcgcc acgctgctcg acgggatgga ggccaacgag cgcttcgtcg ccaccgtccg
     7621 caccgagctc ctcgccgccg actcgaccgg cagcggcacg gtcacgatcg acagcgcggt
     7681 catcgacaac gccctcctgg cggccggcgt gggccgggca ccggccgcgg tcgagttcga
     7741 tccggcggtg atggtggggc tgccccccac ctcgggctac gtcgacgacc ccatctgcgc
     7801 ggccaacggc aacatggtcc accaggacac cgacgtggag ttcccggcca tcgcgggggc
     7861 gctcaccctc acccgcacgt ggaactcact gcgccaccgc cagcccggcg cgttcggacc
     7921 ggggtggtcg tcggtgctcg acgtgcgcct cgccatcggc gacggcgtgg tccgagcgca
     7981 cctcgccgac ggtgccatcg tgcccttcgt cccggtcgac gacagcgccg acggcaggcg
     8041 atgggaggtc cgcggccggc gcggcctgac gctcaccgag atcgacgagg gcttcgagct
     8101 cgacgtcgac ccggtgcggt cgttccggtt cgacggctcc ggcgcgctgc tggggtggcg
     8161 gagcggggtc gccgaggtca ccgtcacccg caacgaccac gggcgcgtca tcgggctgca
     8221 cgagcagcgc accgggcgct ccctcgccgt gcactgggcc ggtggctacg tcgagtcggt
     8281 gaccaccgac gacgggcgca ccgtctccta cgagcgcacg gcgtcggcca ccatcgccac
     8341 ggcccggagc cacgccggat cgctccgcta cgtgtggcac cgcgagacca tgctgcgctc
     8401 ggtcgtcgac gccgacggcg tggccgcgtt cgtgaacgag tacgacgacg cggcccgggt
     8461 gctgcgccag accagcccgc acgggcgccg cacgcagtac cactacgagg cgggcggctc
     8521 ggccgtgatc acgggggacg acggcgtcca gcaggccatg atccacgatg cgcggggcaa
     8581 cctcaccgcc gtcgtcgacc acgacggctc ggtgatgcgc atcgagtacg acgacgccga
     8641 ccgggccgtg cgggtcaccg agcgcgacgg cgccgtctgg cactacgagt acgacgagcg
     8701 cgacgacctg gtccgccgcg tcgaccccga cggggggacc cagacctggg cgtgggacga
     8761 ccgccaccgc gtggtggcca ccaccgaccg ggccggggcc acgacccgga tggagtacga
     8821 caccgaccac caggccccga cccgggtgat cggacccgac ggcgggatca gcacccaggt
     8881 gctcgacgac cgcggcctgc ccctcgagat caccgatccc gacggcgtcg tcacccgctt
     8941 ccgctgggac cgcgacggcc agctggtgtc gaccaccgac gcgctcggca acgagacccg
     9001 gttcgaccac gacgcccacg gcctgctgac cgggctcgtc ccgccggccg gcgcggccac
     9061 ccacctgcag cgcggcgacg acggtcgcgt gcagcgatcg atccgcggcg acgccgtcgc
     9121 gtcgtggacc tacaccccgg ccgggcggat caccgccggt gtcgagcctg gagacgtccc
     9181 gtggtcagcg acgttcggtg cccacggcga cctcgagacc ttcaccgacg gcaccggcgc
     9241 cacggtcgag atgcgctacg actccctcgg caacgtcgtc gaggtcgtcg cccccgacgg
     9301 tgcggtgtac gccagcacct acgacgcggt gggtcgccac gtctccgcga gcgacccctc
     9361 gggcgccacc gtcaccaagg gctacgacca gcgcgggcgg ctggtgtcgt tcaccgacgc
     9421 cgagggcggc acctggatcc gggagctcga cgtcctcggc cggaccgtgg cctccaccgc
     9481 ccccgacggg cgcacgacgc gctggatgca ccacccgcgc ggcgaggtgg catccgtgac
     9541 cacgcccgac gggcgggtct ggaccgtcga cgtggacgtg gcgggccgcc ccgtggccgt
     9601 caccgacccg accggggcgc ggtccgagat cggctacacg ccggcgggtc gcctggcctg
     9661 gcgacgcagc ccggcgggac gcaccgagtc cttcgagtac gacgccgccg gacgattggt
     9721 cgccacggtg gaccccgacg gcacccggcg cgagctgtcg ctcgaccccc tcgggcgggt
     9781 cgtggccgtc gaggacgccg agggtcgact cgagctgtcc tgggacgacg agcaccacct
     9841 cgccgaggcc gccctgcccg gcggcgggac ggccacctac gagcgcgacg ccggcggccg
     9901 ggtgcgccgc ttcaccgacc cgaccggcac gtcgacccgc ttcgagtggg acgagcgggg
     9961 cctgccctcg accgcgttcg accccgccgg gatcccgacc cggttccgcc acgaccagcg
    10021 tggacgcctg cagggcatcg actcgacagg ggatcgcacg ctctcgctgg actacggcat
    10081 cgacggccgc ctcgcccagg tcagcgaccc caccggggtg accaccgcct accggcgcga
    10141 cgccaacggc cggatgaccg gtctgcgcca ccgcgacggc tcggggtggg accgcacgct
    10201 cgacccggtg ggtcgcgagc tcagccggac cagcaccgag ggcgacgtga cccaggcgcg
    10261 cgcctacgac gcggcgggcc ggatcagcgc cgcgaccgtc gaggacctca ccaccgagtt
    10321 cctgtgggac gacgccgacc gcctcacctc ggtgacgggc gtcgacggcg gcacgacgag
    10381 gatcgagcgc gacctcgacg gctgggtggt cgggctggtc gacgtcgact ccggggagcg
    10441 cacgacgttc gagcgcgacg gacggggccg aatcacgagg gtgtcggtgg acggcgaagg
    10501 cgtcgacgtg ccgacccgca gcgagctgcc gcgcgacctc gccggacgcc tcgccatcgg
    10561 cgcggacggg acggcctacc gctacgacga cagcggccgg ctggccgagg tgctgcccca
    10621 ggacgagccc gccaccacct acgcctacga cgacgacggc ctggtcgccg tcgagaccgg
    10681 gccgggcggg atccgccact tcacctacga cgccgccggc cgcgtcgtgg ccgtccgggt
    10741 cgagggcgtc ggcaccaccg acatcgagta cgactcccga gggcgtcggg tgctcgagac
    10801 ccgcccggac cgctcgacgg tccgctacgg gtgggacgcg ctcgaccgcc tggcgtccat
    10861 cgagacccgt cgctggcccg acgacgacga ggccacccgg ctgaccatcc ggtacgacgc
    10921 actcgaccgc ccggtcctcg tcgatggcga ccgtgtcgac cacgaccccg tcaccgggca
    10981 ggtccggcgg ctgggcgacc gccgcttcgt ccccgtcgac tcgatcgcct acgacaccgc
    11041 gacccgatcg tggcaggagc ccggtccccg tcgacccctc ggcggcacgc cggccggccc
    11101 ggtctggatc ctcggggccc gcgtcctcga ccccgccacg caccagttcc tctcccccga
    11161 tcccctgctc ccggtcgccg ggaccaacgg cgcgtccagc gcgtacacgt acgcgtggca
    11221 ggacccggtc aaccacgtgg acccgcgggg cctccggccg gtgtcgatgg aggagtacga
    11281 cgccatccgc cagcgcgagg agcagggtcg cgtcgggcag gcctaccagg ccatccgcga
    11341 ggacccgtgg gggtccctcg ccacggtcgg cgtcgtggcc gccggcgtcg ggctgatgtt
    11401 cgtgcccggc gggcaggtgg tcggcgccgg catcctgatc ggtgcggctg cgggggccgg
    11461 catcggcttc gccaccggca acttctcccc caccggggtc gcggtcagcg gggccttcgg
    11521 cgccatcccc ggcggttcga cgatgcgcag cgcgctcacg gtcggcgccg ccaccggagc
    11581 cggcgagacg gtcgtgacca gcgcgctcac cggccagggg ttcccgagcg ggcgcgagct
    11641 cgtcttcggc acggtcaccg ggagcgcggt gggcggcggc gcccaccgcc tcacgtccgg
    11701 cctggggtcg accgtggacg acctggtgcc ggctcgctcg accgccgcgc tcgaccacgc
    11761 gccgaccgc
//
LOCUS       DATISU010000460        16684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_46230, whole genome shotgun sequence.
ACCESSION   DATISU010000460 DATISU010000000
VERSION     DATISU010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 16684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16684
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46230"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="VLR27_18260"
     CDS             complement(<1..1467)
                     /locus_tag="VLR27_18260"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HSP05454.1"
                     /translation="MLSSEPLLLGAAVLVLGGVLASKLANRLGVPALLLFIGLGMLAG
                     SDGLGGIEFDDVEMTQGIGVAALALILFDGGIATRWEAIRPVLAPGGVLATIGVAITA
                     GITGVLASAILDVPIEVGLLLGAIVSSTDAAAVFSVLRSRRAGLKGGIQPMLELESGA
                     NDPMAVFLTIGLVEIVTGDTDSWWSLLPMFGQQVVLGALLGVLGGWIGRLLLNKVNLG
                     IDGLYPVLTLAVAVATYAGTVLLGGSGFLAVYLCGLWLGNHEVLHRNSVMRFHDAIAW
                     LAQIGMFLVLGLLVFPRDLPDVTWQGLLVATVLVVVARPIAVALSLTPFRVPVRQQAV
                     VAWVGLRGATPIILATFPLVAGVDEAVLLFDVVFFVVLTSVLIQGTTLGPVARLARVT
                     VPVPNRMPAPLEAGEPLPDGTSLRQLEIPSRSFGDGRALVELRLPERALLVLVDRDGT
                     YIVPTGATRLAAGDNVLLLADDGTFRDARDRLTAPDEDA"
     gene            1492..2316
                     /locus_tag="VLR27_18265"
     CDS             1492..2316
                     /locus_tag="VLR27_18265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HSP05455.1"
                     /translation="MDVGIHLPQYGRVASSEAITTAARRAEELGFADVWVSDHIVHPA
                     EQDYPSPFLFDPMVALTWAAAVTERIGLGTSVLVLPSHVPLELANQLASLDALSGGRV
                     TVAGGVGWSEREFDALGHGFADRGPRADEIIDALRVAWTEDPATFEGEHVSFRDVRVL
                     PQPAHAIPIWIGGMSERALRRGVEKGDGHQIIGVKPPEAAPVIERLRRDRPEESFTIS
                     LRTGWDPQGMDPDEIRAEADAYAEAGVQHVVSAPWRSDLDSWLRSMELLAELVLDR"
     gene            2313..3002
                     /locus_tag="VLR27_18270"
     CDS             2313..3002
                     /locus_tag="VLR27_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05456.1"
                     /translation="MSELEIVKPAVDVGIQTNDLEAHQAFWGDELGLRFDHLLKVGGG
                     VHQHRYDLHGGVLKLNAHRDPLPADAPTGYTAIAIVDAARALEGIQGRQGPDGLRVAL
                     TPPTQDGTRVNVMVEASDVDRTLGVLHRTFGGDRVEGTLRIGETKLSVKLRHGRPPTT
                     SRDGLGIRYLTVQVRDVHAAHDHALASGMAEGLAPVRLGDVAHISFVRLPDGDFIELS
                     QRASLTGPLPD"
     gene            complement(3010..3606)
                     /locus_tag="VLR27_18275"
     CDS             complement(3010..3606)
                     /locus_tag="VLR27_18275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HSP05457.1"
                     /translation="MDHHERNDGLDQLPPWTPPADLPAFAAFGPYSELAQPTVSIENP
                     AGIHVGAFVKIGAYAVLEALVPERGIGLRIADAAYIGHFARITAVNEVIIGEEAMVAD
                     RVYVSDTGHVYEDVTQPIKRQGLRDGRRVEIGRGAWICIGAVIVGNVRVGEGAVVGAN
                     AVVTQDVPDFTVVAGNPAQVVRQHDGDDWRWLTPRGPA"
     gene            3735..6662
                     /locus_tag="VLR27_18280"
     CDS             3735..6662
                     /locus_tag="VLR27_18280"
                     /inference="COORDINATES: protein motif:HMM:NF005898.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSP05458.1"
                     /translation="MQLIPREVTRSVVRLGQAAQNALEVARFGGLETGEEHTPYEVVT
                     SHRMYRLRRYGNDGAGDGRRSPVLLVPPMMLSAEIYDVSPTSSAVGALAAAGLDPWVI
                     DFGAPEREEGGLERTLTDHVLALSEAVDEVVERTGGPIHLSGYSQGGMFCYQAAAFRR
                     SEGIASLITYGSPVDTRGVLPPGLDDDLAVEAIDFLADKVLPYFTVPAWMSRAGFRML
                     DPLATARNQLQFVRQLHDRESLLPREGQRRFLMDEGWVAWPGPAIAELVKQFLVSNRM
                     LSGGFVIDEQPVTLADIDCPILCFVGTSDDIAPPEVVRAVAQAAPSALVHEATIDAGH
                     FGLVVGSQAAVTTWPTVTEWTRWLDADGPAPTGTTPLSPDDPPSRRPKSDLARAVNLA
                     TNITAGVGRTLGTGAKIGLRGARSLAMEGVGSLARLNRIEEVRPTSQVSMGRIFAEQA
                     ALAPDDTAFLFEGRGHTYAAANERIDNVVRGLIRIGVRRGSHVGVLMDTRPSALGIVA
                     ALNRLGAVAVLLRPDGPLEREIELGRVGRIIADPEHGEQATRLKGVVKVHVLGGGGGP
                     RDLGSAVDDMEAIDPDSVELPGWYEPNPGRAHDLAFVLFTGSGERTRVNRITNGRWAL
                     SAFGTAASASLNRSDTVLSLTPIHHPSALLTGIGAALASRARLAMTRGFDPDTFWEEV
                     RRYGATAVTYTWTMAADMLDAPTGGRDKHHPVRLFIGSGMPTGLWRRVEQHFAPAKVL
                     EFYASTEGAAVLVNLTGEKVGSMGRRLPGSAPLKIARFDVETSRLIEGPDGFAEECAV
                     GEVGMLLAKLSHRDGAYPDRSLRALFEGDDAWLATGDLFLVDEDGDHWLIGGVQSLIR
                     TPDGATPPIPVQDAVSALDEVHLSVAYPVEQDDGTTAVAVAISLRPGLSVDLDRLAGR
                     LTDAVQVLSPADRPRYVRVVDEIPRTTWYRVRTGPLRAEGIPADGSVLELDDETGTYR
                     PIP"
     gene            complement(6666..7754)
                     /locus_tag="VLR27_18285"
     CDS             complement(6666..7754)
                     /locus_tag="VLR27_18285"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSP05459.1"
                     /translation="MSTDALHELDDDAIRLMAFRVWGYKQGEQVSLLIHLGDRLGLFR
                     AMAGAGPLTVDDLAGRTELHDRWIREWLWAMAAAELVEHTPGEPDRFELTRAGQAVLV
                     DEDSSLFFAAGAFSEPTPPDIIDDLADAFRTGIGLPYDRQGPRGAHRTERMLGPWARL
                     ALVPVILPMLDGVVDKLEAGADVCDVGCGSGVALEAMAKAYPASRFTGYDLSRNAVEI
                     ARQKMADAGIDNVEIVHARAEDLPAEPAFDLVLTFDCLHDMTRPDDALAAVRGSLRDD
                     GTLLVKEIKCADTLEQNKANPMLAMMLGFSITSCMSSAMSEPGGMGLGTVGLPESRLR
                     ELAEAAGFSSVVRHDPGEPSNVYYEVRP"
     gene            7808..8461
                     /locus_tag="VLR27_18290"
     CDS             7808..8461
                     /locus_tag="VLR27_18290"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HSP05460.1"
                     /translation="MVGVDGARGGWVGVRWDGDSLTCAFAATLTGLVDEVWPVAVVAV
                     DMPIELETSTTRACEDQARPLLGARRSSLFQSPSLGALDFADDDYPSANAWSKATTGR
                     GISKQAWFLVPKIREVRALAETCEVPVRECMPELSFRAMHGEPLAHAKTTWSGHALRV
                     RLLREHGIDLPDDPGPAGRVAPDDLTDAAAVAWSARRIAAGTAECAPPEGGAAAIWW"
     gene            complement(8585..9415)
                     /locus_tag="VLR27_18295"
     CDS             complement(8585..9415)
                     /locus_tag="VLR27_18295"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSP05461.1"
                     /translation="MSALQDQAALVTGGGSGIGLACARALLRDGASVTIAGRGEERLR
                     AAVAELEADAPDGAKVGHAVCDVAVEDQVAAAAEQAASLTGSLQLCVAAAGTGTMAPV
                     VAMSSEQWEQVLGVNLTGAFFTLKHVAPRMVAAGGGSFVGISSIAAPLTHRYMSAYCV
                     SKIGLEALIKTAADELGTSNVRCNAVRPGLVPTELSSALEGDPAVHDDYIAQMPLART
                     GTPEDVANAVRYLCGPESGWVTGQALGVDGGHSLRRGPDLEHWSRALFGDDVTEGRVG
                     "
     gene            9441..10688
                     /locus_tag="VLR27_18300"
     CDS             9441..10688
                     /locus_tag="VLR27_18300"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSP05462.1"
                     /translation="MTDDATDAPPGLLDGVVVLDLTRVLAGPICGRALADLGARVIKV
                     EPPAGDLSRFSVPRHGPISGYYAQQNAGKENVSIDLDHADGRALLFDLACRADVVLEN
                     FRPGVCERWGVGYDAVAAKNPGVVYASLTGFGQTGPWAGRRAYAVIGHAVAGTTAGQL
                     QHQEVVAGQAVLANDAYSHGDVYTGLVALSGVLAALFARQSTGRGQHVDVSMVDSLLF
                     VNEHAQEQLAGETEPGDPAILGSGWSPVVEVANGERVSVAGDPAGHGYADFLRLLGRD
                     DLVDDPDMRSWDGRRSQATRLRQDFRDFAATIPTGEDLVALLDEVGTAAGVVRSVPEL
                     AGEDHIVQRGSIVEVDDGAGGTFAIPQAPWRFSDAAAGVAGPPAWRGEHNWTVVTGLL
                     GRTDADVDALEAAGVLVARPPRG"
     gene            10698..11660
                     /gene="ligD"
                     /locus_tag="VLR27_18305"
     CDS             10698..11660
                     /gene="ligD"
                     /locus_tag="VLR27_18305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HSP05463.1"
                     /translation="MPEIPLPMKATAGELPTGDGWVYEVKWDGMRAIVEVEDGHVRLQ
                     SANGKDATVSFPELASLGGALGVRSAVLDGEIVAFDEQGRPSFSRLQQRMHVSDRRAA
                     ATRAETTPCTLAVFDLLALDGRSLIGLPWAERRRLLEDLVEPGPHWQVPAVNDDGQAL
                     LDAATANDLEGIVAKRVDSTYRPGGRTREWVKVKVRRRQEFVVGGWAPGKGNRGGGIG
                     GLLVGYHDEPGSPPLRYAGRVGSGLGQAELRTLEERFADMVVDACPFDPPPPRTHAVD
                     AVWVRPELVVEVEYAEWTNDGRLRHPTYAGRRIDKDADQVTAEP"
     gene            11701..12090
                     /locus_tag="VLR27_18310"
     CDS             11701..12090
                     /locus_tag="VLR27_18310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05464.1"
                     /translation="MDGVTSRDTIRMTIPALLAYVRLPRVAIAGLATRSGFSYDEVED
                     LRLAVGEVCQVLLDGTDRDGTLTIDFTVARGTVGVEVAIDAPPGRHDGLAERLAEQIL
                     AATVGKFEVGDDGRRITFAMSAADDDD"
     gene            complement(12145..12588)
                     /gene="dut"
                     /locus_tag="VLR27_18315"
     CDS             complement(12145..12588)
                     /gene="dut"
                     /locus_tag="VLR27_18315"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HSP05465.1"
                     /translation="MDALTIPVRRLDPDLPLPGYAHEGDAGADLVAREDALLSAGGGR
                     ALVPTGIAIAIPTGWAGFVQPRSGLALRHGVTVLNSPGLIDAGYRDELKVVLVNTDPT
                     EDYRVHRGDRIAQLVLRPVATAAFEPVEELPDSHRSTGGFGSSGR"
     gene            12620..13192
                     /gene="orn"
                     /locus_tag="VLR27_18320"
     CDS             12620..13192
                     /gene="orn"
                     /locus_tag="VLR27_18320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HSP05466.1"
                     /translation="MLAWMDLEMTGLEPERHVIVEIATIITDDDLQIVAEGPDLIVHA
                     TEDQLALMDDVVVRMHTESGLLEKIRSSELSLADAGAQTLAFLQEHIPAARSVPLCGN
                     SIGTDRRFLARYLPEIEDHLHYRSVDVSTIKELAKRWMPSLLQGAPTKVGSHRALDDI
                     RESIEELRYYREALFRDGGTDASPADAPAS"
     gene            complement(13143..14240)
                     /locus_tag="VLR27_18325"
     CDS             complement(13143..14240)
                     /locus_tag="VLR27_18325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSP05467.1"
                     /translation="MSVVDVVLVVVRLAIGVWLLWSLPRLGRLGSFDLDRVSVVVPAR
                     NEAAQLPRLLRSLSGTRAEVVVVDDDSTDGTPSVASSHGASVVSSTPLPDGWGGKAWA
                     CQVGAGAAGGDVLVFVDADVRFAPGVLGRVVAALDEHGGLVSVQPFHVPGSPAEHLAA
                     LFNVVAIAATDVASPTGRRAGARGAFGPVLACRRADLERAGGHAVVGARVNDDVALAE
                     AFRAQGLPVTVLAGGDDVAFRMYPLGLGQLVEGFTKNLAAGAGAVRRTTVLLVVAWLT
                     LLVQASVAPVRALLGSADVLPAAALYAVVAAQVWWMARRVGRFGPITALAFPLLLVTF
                     LAVFARSMVATARGSVSWRGRRLATRPSRRP"
     gene            14360..15436
                     /locus_tag="VLR27_18330"
     CDS             14360..15436
                     /locus_tag="VLR27_18330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSP05468.1"
                     /translation="MGTTVERQERQPASTEVTEVAPDILRMQLPISLPGLGHVNTYAL
                     CDDRGVAVVDPGLPGKASWDALSDRLRQAGYGVKDVHTVIVTHSHPDHFGGAGILRHR
                     VGAEVVTHRAFRTWLDPDEGDDAGPDEITDGDDAVPDQRSASPWQKEMPWRSGQFRPP
                     MKRRLGYRLGRTFGRRWMRAPEPSRRVDDGNILTFAGREWQAVHTPGHTEDHLCLFSA
                     DDGVVLTGDHVLPTITPHISGLHAGHDPLDEFFTSLDRMAELQGATLALPAHGHPFED
                     LTGRCNAIRDHHHERLETLREAARSLGEASVVELSKHLFKQRSWGPMAESETYAHLEH
                     LRRAGEMVSRRVDEDLHFQLVEPA"
     gene            complement(15486..16238)
                     /locus_tag="VLR27_18335"
     CDS             complement(15486..16238)
                     /locus_tag="VLR27_18335"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HSP05469.1"
                     /translation="MAPAGTDDPLAIQPPPEGRITGHRRRVQLADGSPKGRLRLDAAA
                     RMLQDVSDEDTTDAGFPLSEPWVVRRVEMLIAEFPVFRQHVDVRTWCSGIGARWAERR
                     VRVESVEGPGLVEAAVLWVHLGDQGRPTRLPDSFESLYATAARGRKVRARLHHDARPP
                     EGTETWSFPLRFTDFDMMDHVNNAVSWEPVEQALAERPHLRAPLRVSVEHPAPIEAGS
                     EPQVAVADVDGGFDLWVLVDGAACSTARVRTA"
     gene            16394..16624
                     /locus_tag="VLR27_18340"
     CDS             16394..16624
                     /locus_tag="VLR27_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05470.1"
                     /translation="MKTTLLHLLQTAASVVAPRPKTAVLALAPVCAGAPAYGPTETVV
                     IGNGITGSWLATSRLSMTVPAFHDRRPLCSTT"
BASE COUNT         2397 a         6229 c         5967 g         2091 t
ORIGIN      
        1 ggcgtcctcg tcgggcgcag tgagccggtc ccgggcgtcg cggaaggtgc cgtcgtcggc
       61 gagcaggagg acgttgtcgc cggcggcgag gcgggtcgca ccggtgggga cgatgtaggt
      121 gccgtcccgg tcgacgagca cgagcaacgc ccgttcgggc aggcggagct cgaccagggc
      181 acggccgtcg ccgaaggacc gggacgggat ctcgagctgg cgcagggagg tcccgtcggg
      241 gagcggctcg ccggcttcca ggggtgcggg catccggttc ggcacgggga ccgtgacgcg
      301 ggcgaggcgg gcgaccggcc cgagggtggt cccctggatg agcaccgagg tcagcacgac
      361 gaagaagacg acgtcgaaga gcaagacggc ctcgtccacg ccggcgacga gcgggaacgt
      421 ggccaggatg atcggcgtgg cgccgcggag gccgacccag gccaccacgg cctgctggcg
      481 gaccggcacc cggaagggtg tgagcgagag cgcgacggcg atgggtcggg cgacgacgac
      541 gaggacggtg gcgacgagca ggccctgcca ggtgacgtcg ggcaggtccc tcgggaagac
      601 gagcagaccg aggacgagga acatgccgat ctgggccagc cacgcgatgg cgtcgtggaa
      661 gcgcatgacg gagttgcggt gcagcacctc gtggttcccc agccacagcc cgcagaggta
      721 gacggcgagg aagccgctgc cgccgagcag cacggtgccg gcataggtgg cgacggcgac
      781 cgccagggtg agcaccgggt agaggccgtc gatcccgagg ttgaccttgt tgagcagcag
      841 ccggccgatc cagccaccga gcacgccgag caacgcgccg agcaccacct gctggccgaa
      901 catcgggagc agggaccacc agctgtcggt gtcgccggtg acgatctcga ccaggccgat
      961 ggtgaggaag acggccatcg ggtcgttggc gccggactcg agctcgagca tcggctggat
     1021 cccgcccttg agccccgccc ggcgggagcg cagcaccgag aacaccgcag cagcgtcggt
     1081 ggaggacacg atggcgccga gcaggaggcc gacctcgatc ggcacgtcga ggatcgccga
     1141 ggcgagcaca ccggtgatcc cggcggtgat ggcgacgccg atggtggcga gcaccccgcc
     1201 gggggcgagc acgggacgga tggcctccca gcgcgtggcg atcccgccgt cgaagaggat
     1261 gagggccagg gcggcgaccc cgatgccctg ggtcatctcg acgtcgtcga actcgatgcc
     1321 gcccaacccg tcggacccgg cgagcatgcc gaggccgatg aacagcagga gcgcggggac
     1381 cccgagccgg ttggccagct tcgacgcgag cacgcccccc agcaccagca cggccgcccc
     1441 gaggaggagg ggctcggagc tcagcacgat cgacagcgta cggtcggcct catggacgtc
     1501 ggcatccacc tcccccagta cggacgggtc gcctccagcg aggccatcac gacggcggcg
     1561 cggcgggccg aggagctcgg cttcgccgac gtgtgggtca gcgaccacat cgtccacccg
     1621 gccgagcagg actacccgtc gccgttcctg ttcgacccga tggtggccct cacgtgggcg
     1681 gcggccgtca ccgagcggat cggtctcggc accagcgtgc tggtcctgcc cagccacgtg
     1741 ccgctcgagc tggccaacca gctggcctcc ctcgacgcgc tgagcggggg ccgggtcacg
     1801 gtcgccggcg gcgtgggttg gtccgagcgg gagttcgatg ccctcggcca cggcttcgcc
     1861 gaccgggggc cgcgcgccga cgagatcatc gacgcgctgc gggtcgcgtg gaccgaggac
     1921 ccggccacgt tcgagggcga gcacgtctcg ttccgcgacg tccgggtgct gccgcagccg
     1981 gcgcacgcga tcccgatctg gatcggcggc atgagcgagc gggcgctgcg acgtggcgtc
     2041 gagaagggcg acggccacca gatcatcggc gtcaagcccc ccgaggccgc accggtcatc
     2101 gagcggctcc gacgggaccg cccggaggag tccttcacca tctccctgcg caccggctgg
     2161 gacccccaag ggatggaccc cgacgagatc cgggccgagg ccgacgccta tgccgaggcc
     2221 ggggtgcagc acgtggtgtc ggcgccgtgg cgcagcgacc tcgacagctg gctccgctcg
     2281 atggagctgc tcgccgagct ggtgctggac cgatgagcga gctcgagatc gtcaagccgg
     2341 ccgtcgacgt cggcatccag accaacgacc tcgaggccca ccaggcgttc tggggcgacg
     2401 agctcgggct gcggttcgac cacctcctga aggtcggcgg gggcgtgcac cagcaccgct
     2461 acgacctgca cggcggcgtg ctgaagctca acgcccaccg cgacccgctg ccggcggacg
     2521 cgccgaccgg ctacaccgcc atcgccatcg tcgatgcggc ccgggcactg gagggcatcc
     2581 agggtcgcca ggggcctgac ggcctgcgcg tcgccctgac gccgccgacc caggacggca
     2641 cccgggtgaa cgtgatggtc gaggcatccg acgtcgaccg gaccctcggc gtgctgcacc
     2701 gcaccttcgg gggagaccgg gtcgagggca ccctccgcat cggcgagacc aagctcagcg
     2761 tgaagctgcg ccacggccgg ccgccgacga cctcccgtga cggcctcggc atccgctacc
     2821 tcaccgtcca ggtccgcgac gtgcacgccg cccacgacca cgccctcgcc agcgggatgg
     2881 ccgaggggct ggcacccgtg cggctcggcg acgtggccca catctccttc gtgcgcctgc
     2941 cggacggcga cttcatcgag ctgtcccagc gagcctcgct caccggcccg ctccccgact
     3001 gagcgccggt caggcgggac cgcggggcgt gagccagcgc cagtcgtcgc catcgtgctg
     3061 gcgcacgacc tgggccgggt tgccggccac gacggtgaag tccggaacgt cctgggtgac
     3121 cacggcgttc gcccccacca ccgcaccctc gccgacccgc acgttgccga cgatgaccgc
     3181 cccgatgcag atccacgccc cccgaccgat ctccacgcga cgtccgtctc gcagaccctg
     3241 gcgcttgatc ggctgggtga cgtcctcgta cacgtgcccg gtgtcggaca cgtacacccg
     3301 gtccgccacc atcgcctcct cgccgatgat cacctcgttc acggcggtga tgcgcgcgaa
     3361 gtgcccgatg taggcggcat cagcgatgcg cagaccgatc ccgcgctcgg gcaccagcgc
     3421 ctcgaggacc gcgtaggcgc cgatcttcac gaacgcgccg acgtggatgc cggcggggtt
     3481 ctcgatcgag accgtcggct gggcgagctc gctgtacggg ccgaaggccg caaaggcggg
     3541 caggtcggcc ggcggcgtcc aaggcggcag ctggtcgagg ccgtcgttgc gctcgtggtg
     3601 gtccaccgcc acagtgtccc cgaccgggcc ggcgggatcg gtgacccgtc gaacccgaca
     3661 tttcggtcac gcgaaggatc accggtagcc tgcgcggcac aagccgccac agacacggat
     3721 cccgaggtac cccggtgcag ctgatcccga gagaggtcac ccggtcggtg gtacggctcg
     3781 ggcaggcggc gcagaacgcg ctcgaggtcg cccgcttcgg cgggttggag accggcgagg
     3841 agcacacgcc ctacgaggtc gtcacctcgc accgcatgta ccgcctgcgc cgctacggca
     3901 acgacggcgc cggcgacggg cgccgctcgc ccgtgctgct ggtgccgccg atgatgctgt
     3961 cggccgagat ctacgacgtg tcgccgacgt ccagcgcggt cggcgccctc gccgctgccg
     4021 ggctggaccc gtgggtcatc gacttcggcg cccccgagcg ggaggagggc ggcctcgagc
     4081 gcacgctcac cgaccacgtg ctcgccctgt ccgaggcggt cgacgaggtc gtcgagcgca
     4141 ccggcggccc gatccacctc tccggctaca gccagggcgg gatgttctgc taccaggcgg
     4201 ctgcgttccg ccggtccgag ggcatcgcga gcctcatcac ctacggcagc ccggtcgaca
     4261 cgcggggcgt gctgcctccc ggcctcgacg acgacctcgc cgtggaggcc atcgacttcc
     4321 tcgccgacaa ggtcctcccc tacttcaccg tcccggcgtg gatgagccgc gccggcttcc
     4381 ggatgctcga tcccctggcc accgcccgga accagctgca gttcgtgcgc cagctccacg
     4441 accgcgagtc gctcctgccg cgcgagggcc agcgccggtt cctcatggac gagggttggg
     4501 tggcctggcc cggcccggcc atcgccgagc tggtgaagca gttcctcgtg tccaaccgca
     4561 tgctgtcggg cgggttcgtg atcgacgagc agcccgtcac cctcgccgac atcgactgcc
     4621 cgatcctgtg cttcgtcggc acctctgacg acatcgcccc gcctgaggtc gtccgcgccg
     4681 tcgcccaggc cgccccgagc gccttggtcc acgaggccac catcgacgcc ggccacttcg
     4741 gcctggtggt cggctcccag gcagcggtga ccacctggcc caccgtcacc gagtggaccc
     4801 ggtggctcga cgccgacggc cccgcgccga ccggcaccac gccgctctcc cccgacgacc
     4861 ccccgagccg acggcccaag agcgacctgg cccgagcggt caacctcgcc accaacatca
     4921 ccgccggcgt cggtcgcacc ctcggcaccg gcgccaagat cggcctccgt ggcgcgcgct
     4981 cgctcgccat ggagggggtc ggctccctgg cgcgcctcaa ccgcatcgag gaggtccgcc
     5041 ccaccagcca ggtgtcgatg ggtcgcatct tcgccgagca ggccgcgctc gcccccgacg
     5101 acaccgcctt cctgttcgag gggcgcggcc acacctacgc cgcggccaac gagcgcatcg
     5161 acaacgtggt gcgcgggctg atccgcatcg gcgtgcggcg tggctcccac gtgggcgtgc
     5221 tcatggacac ccggccgagc gcgctcggca tcgtggccgc cctcaaccgg ctcggcgcgg
     5281 tcgccgtgct cctgcgtccc gacggccccc tggagcgcga gatcgagctc ggccgggtcg
     5341 ggcgcatcat cgccgacccc gagcacggcg agcaggccac ccgcctgaag ggcgtggtga
     5401 aggtgcacgt gctcggcggt ggcggcggac cccgcgacct cggttcggcc gtcgacgaca
     5461 tggaggccat cgaccccgac tcggtcgagc tgccggggtg gtacgaaccg aacccggggc
     5521 gggcccacga cctggccttc gtcctgttca ccggcagcgg cgagcgcacc cgcgtcaacc
     5581 gcatcaccaa cgggcgatgg gccctgtcgg ccttcggcac agcggcgtcg gcctcgctca
     5641 accgatcgga cacggtgctc agcctcacgc cgatccacca cccgtccgcc ctcctcaccg
     5701 gcatcggcgc cgcgctggcg tcccgggccc ggctggccat gacccgcggc ttcgaccccg
     5761 acacgttctg ggaggaggtg cgccgctacg gcgccaccgc ggtcacctac acctggacga
     5821 tggcggccga catgctcgac gcgccgaccg gcggccgcga caagcaccac ccggtccggc
     5881 tcttcatcgg ctccggcatg cccaccggcc tgtggcgccg ggtcgagcag cacttcgccc
     5941 cggccaaggt cctggagttc tacgcgtcca ccgagggcgc tgctgtgctg gtcaacctca
     6001 ccggcgagaa ggtcgggtcc atgggccggc gcctgcccgg gagcgccccg ctgaagatcg
     6061 cccgcttcga cgtcgagacc agccggctca tcgaggggcc cgacggcttc gccgaggagt
     6121 gcgccgtcgg cgaggtcggc atgctgctgg ccaagctcag ccaccgcgac ggcgcctacc
     6181 ccgaccgctc gctccgggcg ctgttcgagg gcgacgacgc ctggctcgcc accggcgacc
     6241 tgttcctcgt cgacgaggac ggggaccact ggctgatcgg cggcgtccag tcgctgatcc
     6301 gcaccccgga cggcgccacg ccacccatcc ccgtccagga cgcggtgagc gcgctggacg
     6361 aggtccacct cagcgtcgcc tacccggtcg agcaggacga cgggaccacc gccgtcgccg
     6421 tggcgatctc gctgcggccc gggctctccg tcgacctcga ccgcctggcc ggccggctga
     6481 cagacgcggt gcaggtcctc tcgcccgccg accggccccg gtacgtgcgg gtcgtcgacg
     6541 agatcccgcg caccacctgg taccgggtgc gcaccggccc gctgcgcgcc gagggcatcc
     6601 cggccgacgg ctcggtgctc gagctcgacg acgagaccgg cacctaccgg cccatcccct
     6661 gatcgctacg ggcgcacctc gtagtagacg ttcgacggtt cgcccgggtc gtggcgcacc
     6721 acgctggaga agccggccgc ctcggccagc tcgcgcaggc ggctctcggg caggccgacg
     6781 gtgccgaggc ccatgccgcc gggttcgctc atggccgacg acatgcacga ggtgatcgag
     6841 aacccgagca tcatcgccag catcgggttg gccttgttct gctcgagggt gtcggcgcac
     6901 ttgatctcct tcacgaggag cgtgccgtcg tctcgcagcg acccccggac ggcggcgagc
     6961 gcgtcgtcgg gacgggtcat gtcgtggagg cagtcgaagg tgagcaccag gtcgaacgcg
     7021 ggctcggccg ggagatcctc ggcccgggcg tgcacgatct cgacgttgtc gatcccggca
     7081 tcggccatct tctgccgggc gatctcgacg gcgttgcggg acaggtcgta gccggtgaac
     7141 cgggacgccg ggtacgcctt cgccatcgcc tcgagggcga cgccggaccc gcagccgacg
     7201 tcgcacacgt ccgccccggc ctccagcttg tcgaccacgc cgtcgagcat cggcaggatc
     7261 accgggacga gggcgaggcg ggcccacggg ccgagcatgc gctcggtgcg atgggcgccc
     7321 cggggcccct ggcggtcgta ggggaggccg atgccggtgc ggaacgcgtc ggcgaggtcg
     7381 tcgatgatgt cgggcggggt gggttcgctg aacgcccccg cggcgaagaa cagcgagctg
     7441 tcctcgtcga cgagcacggc ctggccggcg cgggtcagct cgaagcggtc gggctcgccc
     7501 ggcgtgtgct cgaccagctc ggcggcggcc atcgcccaca gccactcgcg gatccagcgg
     7561 tcgtgcagct cggtgcgtcc ggccaggtcg tccaccgtca gcgggcccgc accggccatg
     7621 gcccggaaga gcccgagccg gtccccgagg tggatgagga gcgacacctg ctcgccctgc
     7681 ttgtagcccc acacccggaa ggccatgagg cggatggcgt cgtcgtccag ctcgtgcagt
     7741 gcgtcggtgc tcatggtcgg gcagggtggc acacgtgatg cgccgacgtt cgggagacgg
     7801 gtctgccgtg gtcggtgtcg acggtgcccg cggcggctgg gtcggtgtcc gctgggacgg
     7861 cgactcgctc acgtgcgcgt tcgccgcgac gctcaccggc ctggtcgacg aggtctggcc
     7921 ggtggccgtg gtggccgtcg acatgccgat cgagctggag acctcgacca cccgggcctg
     7981 cgaggaccag gcccgacccc tgctcggcgc ccgccggtcg agcctgttcc agtccccgtc
     8041 gctcggcgcg ctcgacttcg cagacgacga ctacccgagc gccaacgcct ggtcgaaggc
     8101 cacgaccggg cgcggcatct ccaagcaggc ctggttcctg gtccccaaga tccgtgaggt
     8161 ccgggcgctg gccgagacct gcgaggtgcc ggtgcgcgag tgcatgccgg agctgtcgtt
     8221 ccgggccatg cacggcgagc cgctggccca cgccaagacg acctggtccg gccacgccct
     8281 gcgggtccgc ctcctgcgcg agcacggcat cgatctcccc gacgaccccg gccccgccgg
     8341 ccgcgtcgcc cccgacgacc tcaccgacgc cgccgccgtc gcctggtcag cccggcggat
     8401 cgccgccggt accgccgagt gcgcaccccc cgagggcggg gcagcagcca tctggtggta
     8461 gggcggccgg cacaacccgg acagaaactg gcagttgaga ggtcacggcg ggcttctgtg
     8521 acctcacaac tgccagtttc cagagtcgga acacaggagg aggacgaacc cgccgccagc
     8581 aacctcagcc gacgcgccct tcggtgacgt cgtcgccgaa cagcgcccgg gaccagtgct
     8641 cgaggtccgg cccacgccgc agcgagtgcc caccgtcgac gccgagggcc tggccggtga
     8701 cccagcccga ctcgggaccg cacaggtagc ggaccgcgtt ggccacgtcc tcgggggtgc
     8761 cggtgcgagc gaggggcatc tgggcgatgt agtcgtcgtg gacggcgggg tcgccctcga
     8821 gcgcggacga cagctcggtg gggaccaggc cgggtcgcac ggcgttgcag cgcacgttgg
     8881 aggtgccgag ctcgtcggct gcggtcttga tgagggcctc gaggccgatc ttggacacgc
     8941 agtaggccga catgtagcgg tgcgtgagcg gggcggcgat cgaggagatg ccgacgaagg
     9001 acccgccacc ggccgccacc atgcgcgggg ccacgtgctt gagggtgaag aaggcgccgg
     9061 tgaggttcac gccgagcacc tgctcccact gctccgacga catggccacg accggcgcca
     9121 tcgtcccggt gccggcggcg gcgacgcaca gctgcagcga gccggtgagc gacgcggcct
     9181 gctcggcggc cgcggcgacc tggtcctcga cggcgacgtc gcagacggcg tggccgacct
     9241 ttgcgccgtc gggcgcatcg gcctcgagct cggcgacggc ggcccgcagc cgctcctcac
     9301 cgcggccggc gatggtgacc gacgcgccgt cgcgcaggag ggcgcgagcg caggcgaggc
     9361 cgatgccgct cccaccaccg gtgacgaggg cggcctgatc ctggagagct gacatggagg
     9421 gtgaacctag agtccggtcg gtgaccgacg acgccaccga cgcacccccc ggactgctcg
     9481 acggggtggt cgtgctcgac ctcacccggg tcctggccgg gccgatctgc ggccgggcgc
     9541 tcgccgacct gggggcacgg gtcatcaagg tcgagccgcc cgcgggcgac ctcagccggt
     9601 tctccgtccc ccgccacggc cccatctcgg gctactacgc ccagcagaac gccgggaagg
     9661 agaacgtgtc gatcgacctc gaccacgccg acgggcgcgc cctgctgttc gacctggcct
     9721 gtcgagccga cgtggtgctc gagaacttcc ggccgggtgt ctgcgagcgc tggggcgtgg
     9781 gctacgacgc ggtggccgcc aagaaccccg gtgtcgtcta cgcctcgctc accgggttcg
     9841 gccagaccgg gccgtgggcc ggccgccgtg cctacgcggt gatcggccac gccgtcgccg
     9901 gcaccaccgc cggccagctc cagcaccagg aggtcgtggc cgggcaggcg gtgctggcca
     9961 acgacgccta ctcccacggc gacgtctaca ccgggctcgt cgcgctgtcc ggcgtgctcg
    10021 ctgccctctt cgcccggcag tccaccggcc ggggccagca cgtcgacgtc tccatggtcg
    10081 actcgctcct cttcgtcaac gagcacgccc aggagcagct ggccggggag accgagccgg
    10141 gcgatccggc gatcctgggc agcgggtggt ccccggtcgt cgaggtggcc aacggcgagc
    10201 gggtgagcgt cgccggcgac ccggccgggc acggctacgc cgacttcctc cgcctgctgg
    10261 gtcgcgacga cctcgtcgac gaccccgaca tgcgctcgtg ggacgggcgg cgcagccagg
    10321 cgacccgcct gcgccaggac ttccgggact tcgccgccac catcccgacc ggcgaggacc
    10381 tggtggctct cctcgacgag gtcggcaccg ccgccggcgt ggtgcggtcg gtcccggagc
    10441 tggcaggcga ggaccacatc gtgcagcggg gcagcatcgt cgaggtcgac gacggcgccg
    10501 gcggcacctt cgccatcccc caggcgccct ggcggttctc ggatgccgcg gccggggtcg
    10561 ccggtccgcc ggcgtggcgg ggcgagcaca actggacggt cgtcaccggc ctgctcgggc
    10621 gcaccgacgc cgacgtcgac gcgctggagg ccgcgggcgt gctggtcgcc cgaccgccac
    10681 gggggtagga accggtggtg cccgagatcc cgctgcccat gaaggcgacc gccggcgagc
    10741 tgccgacggg cgatggctgg gtctacgagg tcaagtggga cgggatgcgc gccatcgtcg
    10801 aggtcgagga cggtcacgtc cgtctgcagt cggccaacgg caaggacgcc accgtgtcgt
    10861 tcccggagct ggcatcgctc ggcggggcgc tcggcgtccg gtcggcggtg ctcgacggcg
    10921 agatcgtcgc gttcgacgag cagggccgcc cgtcgttcag ccgcctccag cagcgcatgc
    10981 acgtcagcga ccggcgcgcc gcagccaccc gggccgagac gacgccgtgc acgctcgccg
    11041 tgttcgacct gctcgccttg gacgggcggt ccctgatcgg gctcccctgg gccgagcggc
    11101 gccggctgct ggaggacctg gtggagccgg gaccgcactg gcaggtcccc gccgtgaacg
    11161 acgacggcca ggccctgctc gacgcggcca cggcgaacga cctcgagggc atcgtggcca
    11221 agcgcgtcga ctcgacctac cgccccggcg gacggacccg ggagtgggtg aaggtcaagg
    11281 tgcgccggcg ccaggagttc gtggtcgggg gttgggcgcc gggcaagggc aaccgcggcg
    11341 ggggcatcgg cggcctgctt gtcggctacc acgacgaacc gggcagtccc ccgctccgct
    11401 acgccggccg ggtcgggagc gggctcggcc aggccgagct gcgcacgctc gaggagcggt
    11461 tcgccgacat ggtcgtggac gcgtgcccgt tcgatcctcc gccccctcgg acccatgcgg
    11521 tggacgcggt ctgggtgcgt cccgagctgg tcgtcgaggt cgagtacgcc gagtggacga
    11581 acgacggtcg cctccgccac ccgacctacg ccggtcgccg catcgacaag gacgccgacc
    11641 aggtgacggc cgagccgtga ttcggtcggt cggtggcggg gtgcgcgagg atcgggcccg
    11701 atggacgggg tgacgagccg ggacacgatc cgcatgacga tcccggccct gctcgcgtac
    11761 gtccgcctgc cccgggtcgc catcgccggc ctcgccaccc gatccggctt ctcctacgac
    11821 gaggtcgagg acctgcgtct cgccgtcggc gaggtgtgcc aggtgctgct cgacggcacc
    11881 gatcgggacg gcacgctcac catcgacttc accgtcgccc gcggcacggt cggcgtggag
    11941 gtcgccatcg acgccccgcc cgggcgccac gacggcctcg ccgagcggct cgccgagcag
    12001 atcctcgccg ccacggtcgg gaagttcgag gtcggcgacg acggccgccg catcaccttc
    12061 gcgatgagcg ccgccgacga cgacgactag cgatctggag cacctcccac gaccgcagcg
    12121 gtcccgacgg gtgccccaga acggtcaccg accgctggag ccgaagccgc ccgtgctgcg
    12181 atggctgtcg ggcagctcct ccaccggttc gaacgccgcg gtggccacgg ggcgcagcac
    12241 gagctgggcg atgcggtcgc cgcggtgcac ccggtagtcc tcggtcgggt cggtgttgac
    12301 gaggacgacc ttcagctcgt ctcggtagcc ggcgtcgatc aggccggggc tgttcagcac
    12361 cgtcacgccg tggcggaggg ccaggccgga gcggggctgc acgaagccgg cccacccggt
    12421 ggggatggcg atggcgatgc cggtcggcac cagggcccgt ccgccgcccg cggacagcag
    12481 cgcgtcctcc cgggcgacga ggtcggcgcc ggcgtcgccc tcgtgggcgt agccgggcag
    12541 cgggaggtcg gggtcgagcc ggcggaccgg gatggtgagg gcgtccaccc cgccaaggta
    12601 gggcggtagc gtcgccacga tgttggcctg gatggacctg gagatgaccg gactcgaacc
    12661 ggagcgccac gtgatcgtgg agatcgccac gatcatcacc gacgacgacc tccagatcgt
    12721 cgccgagggt cccgacctca tcgtccacgc caccgaggac cagctggcgc tcatggacga
    12781 cgtcgtcgtg cgcatgcaca ccgagtcggg tctgctggag aagatccgca gctccgagct
    12841 gagcctcgcc gacgccggcg cccagaccct ggccttcctg caggagcaca tcccggcggc
    12901 gcgctcggtg cccctgtgcg ggaactccat cggcaccgac cggcgcttcc tcgcccggta
    12961 cctgcccgag atcgaggacc acctccacta ccgcagcgtc gacgtctcca ccatcaagga
    13021 gctggccaag cgctggatgc cctcgctgct ccagggcgcc ccgaccaagg tcggctcgca
    13081 ccgggccctg gacgacatcc gggagtccat cgaggagctg cgctactacc gcgaggccct
    13141 gttcagggac ggcgggacgg acgcgtcgcc agccgacgcc cccgccagct gaccgacccc
    13201 cgggccgtcg ccaccatcga gcgggcgaac acggcgagga acgtgacgag cagcagcggg
    13261 aacgccagcg cggtgatcgg gccgaaccgg ccgacccgcc gggccatcca ccacacctgg
    13321 gcggcgacca ccgcgtagag ggcggcggcg ggcagcacgt ccgccgaccc gaggagggcg
    13381 cggaccgggg cgacgctcgc ctgcaccagc agggtgagcc aggccaccac caggagcacg
    13441 gtggtgcggc ggacggcacc cgcgccggcg gccaggttct tggtgaaccc ctcgaccagc
    13501 tgcccgaggc cgagcgggta catccggaac gccacgtcgt cgccgccggc caggacggtg
    13561 accggcaggc cctgggcgcg gaacgcctcg gccagggcga cgtcgtcgtt cacccgggcg
    13621 ccgaccacgg cgtgcccacc ggcgcgctcg aggtccgccc gacggcaggc cagcaccgga
    13681 ccgaacgcgc cgcgagcccc ggcccgccgg ccggtggggc tggccacgtc ggtcgccgcg
    13741 atcgccacga cgttgaagag cgccgccagg tgctccgccg gcgaccccgg cacgtggaag
    13801 ggctgcacgc tgaccaaccc gccgtgctcg tcgagcgcgg cgacgacccg accgagcacg
    13861 cccggcgcga agcgcacgtc ggcgtcgacg aagacgagga cgtcgccgcc ggcagccccg
    13921 gccccgacct ggcacgccca cgccttccca ccccagccgt ccggcagcgg ggtagacgag
    13981 acgacgctcg ccccgtgcga cgaggcgacg ctcggggtgc cgtcggtgga gtcgtcgtcg
    14041 accacgacca cctcggcccg ggtgcccgac agcgaccgca gcagccgggg cagctgcgcc
    14101 gcctcgttgc gggccggcac gaccaccgag acccgatcca ggtcgaagga cccgagccgc
    14161 ccgagccggg gcagcgacca cagcagccag acgccgatcg ccagccgcac gacgacgagc
    14221 accacgtcca ccacgctcac cggaccccca cccccgaccc gttctggcct acatccatga
    14281 ccaccaggcg gcacggatgt aggccagaac gacgaccggc gggcgcactt gaccgaacgg
    14341 tcaagtatgg tgcggcgcca tggggacgac cgtcgaacgc caggagcgcc agcccgcgtc
    14401 gaccgaggtc accgaggtgg cgccggacat cctgcggatg cagctgccca tctcgctgcc
    14461 cggcctcggc cacgtgaaca cctacgccct gtgcgacgac cggggcgtgg ccgtggtcga
    14521 tcccgggctg cccggcaagg cgtcgtggga cgcgctgtcc gatcgcctgc gccaggccgg
    14581 ctacggggtc aaggacgtcc acaccgtcat cgtgacgcac agccaccccg accacttcgg
    14641 tggggccggc atcctgcgcc accgggtcgg cgccgaggtg gtcacccacc gcgccttccg
    14701 cacctggctc gaccccgacg agggtgacga cgccggcccc gacgagatca ccgacggcga
    14761 cgacgccgtc cccgaccagc ggtcggcctc gccgtggcag aaggagatgc cgtggcggtc
    14821 ggggcagttc cgcccgccga tgaagcgccg cctcggctac cgcctgggcc gcaccttcgg
    14881 ccggcgctgg atgcgcgctc ccgagccgag ccggcgcgtc gacgacggca acatcctcac
    14941 cttcgccggt cgggagtggc aggccgtgca cacgccgggt cacaccgagg accacctctg
    15001 cctgttctcc gccgacgacg gcgtcgtgct caccggcgac cacgtgctgc ccaccatcac
    15061 gccgcacatc tccggcctcc acgccggcca cgacccgctg gacgagttct tcaccagcct
    15121 cgaccgcatg gccgagctgc agggcgccac cctcgccctc cccgcccacg gccacccgtt
    15181 cgaggacctc accggccgct gcaacgcgat ccgggaccac caccacgagc gtctcgagac
    15241 cctgcgcgag gcggcccgat cgctcggcga ggccagcgtg gtcgagctgt ccaagcacct
    15301 gttcaagcag cggtcgtggg gtccgatggc cgagtccgag acctacgccc acctcgagca
    15361 cctccgccgg gccggcgaga tggtcagccg ccgggtggac gaggacctgc acttccagct
    15421 ggtcgaaccg gcctgacgcc ggcgcaacac cagcactgcc agaacgatca ggactgcccg
    15481 aacgatcagg cggtgcggac ccgggcggtc gagcaggccg cgccgtcgac caggacccac
    15541 aggtcgaagc cgccgtcgac gtcggccacc gccacctgcg gttcgctgcc cgcctcgatc
    15601 ggggcggggt gctccaccga cacccgcagc ggggcccgca ggtgcggccg ctcggcgagg
    15661 gcctgctcca ccggctccca cgagaccgcg ttgttgacgt ggtccatcat gtcgaagtcg
    15721 gtgaagcgca gcgggaagga ccaggtctcg gtcccctccg gtggccgggc gtcgtggtgc
    15781 aaccgggccc ggaccttgcg gcctcgggcc gccgtggcgt acagcgactc gaagctgtcg
    15841 ggcaggcggg tgggccgccc ctgatcaccc aggtgcaccc acagcaccgc ggcctccacg
    15901 aggcccggac cctcgaccga ctccacccgc acgcggcgct cggcccaccg ggcgccgatg
    15961 cccgagcacc acgtgcggac gtccacgtgc tggcggaaca cggggaactc cgcgatcagc
    16021 atctcgaccc ggcgcaccac ccacggctcg ctcagcggga agccggcgtc ggtggtgtcc
    16081 tcgtcgctca cgtcctgcag catccgggcc gcggcgtcga gccgcagccg ccccttgggc
    16141 gagccgtcgg ccagctggac ccgccggcgg tgacccgtga tgcgcccctc cgggggcggc
    16201 tggatggcga ggggatcgtc ggttccggcg ggggccatcg gggcatgttg cccgagcgct
    16261 gccgacttct tcgataattc gattgaccag gtcctggcgt tgtgatgcca tgaccggcac
    16321 ggctgcgaca gggggctctc cctctccggc ctcggacctg cccaccgcac gcacccggag
    16381 cgaggtgaac acgatgaaga cgaccctcct gcacctgctg cagaccgccg cctccgtcgt
    16441 cgcaccgcgc ccgaagaccg ccgtgctcgc gctggctccc gtctgcgccg gtgcgcccgc
    16501 ctatggcccg accgagacgg tcgtcatcgg caacggcatc accggttcct ggctcgccac
    16561 ctcgcgcctc tccatgaccg tgccggcgtt ccacgatcga aggcctctgt gttccaccac
    16621 ctgaccactg gtcaggcacc aaggaccgca gaggccgtcg ggaacatccc gacggccttt
    16681 cgct
//
LOCUS       DATISU010000461         7571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_510747, whole genome shotgun sequence.
ACCESSION   DATISU010000461 DATISU010000000
VERSION     DATISU010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510747"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VLR27_18345"
     CDS             <1..201
                     /locus_tag="VLR27_18345"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HSP05471.1"
                     /translation="GKNYEDHVVIDEKFYRPAEVDLLIGDATKAEKQLGWKPKTSFPE
                     LVAMMVDADIALLKGDLKGISH"
     gene            198..1136
                     /gene="cofD"
                     /locus_tag="VLR27_18350"
     CDS             198..1136
                     /gene="cofD"
                     /locus_tag="VLR27_18350"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HSP05472.1"
                     /translation="MSDTAVLCGGVGAARFLRGLLAVVPPSSVTAIVNVADDTELHGL
                     HISPDIDTVTYTLADAIDPERGWGLRDETWHAMTMFERYGNPSWFNLGDKDLATHIVR
                     TERLRAGQALSEVTAHLARAWDLECTLLPVTDDRLRTFVTTEHGELSFQEYFVGRQHA
                     VPISGVRFEGADTARPAPGVLDALAGADRIVVAPSNPIVSIGPLLALDEVREALVRHR
                     DRVVAVSPIVAGAALKGPADRMLTELGHEPTVVGVARLYRDIAATLVIDTADAAHAPA
                     VEAEGMACVVTGTVMSDTERSAALARAVLTASGAER"
     gene            1133..1870
                     /gene="cofE"
                     /locus_tag="VLR27_18355"
     CDS             1133..1870
                     /gene="cofE"
                     /locus_tag="VLR27_18355"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HSP05473.1"
                     /translation="MTVSPPPAPGELRILPVHGIGEIRPRDDLAGIIADACNAVPATA
                     LVDGDVLVVTQKIVSKAEDRIEVIDTDDPRGHKAIVERESVRILRRRGDLIIAETSHG
                     FVCANAGVDLSNIEAGYAALLPVDSDRSARRIRDAIRHRLGVEVAVIVSDTFGRPWRR
                     GLTDVAIGCAGIRPIADLRGSTDSLGRELQVTEVCIVDELAGAADLVCGKASGVPVAL
                     CRGIDPAWLGDGSVADLIRHPSEDLFR"
     gene            1867..2589
                     /locus_tag="VLR27_18360"
     CDS             1867..2589
                     /locus_tag="VLR27_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSP05474.1"
                     /translation="MIPLRHEPRPCLSVVMPAYNEQATVAAAIEAVLASPWVAQLVVV
                     DDCSTDGTSAILDGITDERVTRLRHGVNQGKGAALRTGIAHATSEFVVIQDADLEYDP
                     AEYAKLVQPLLDGSADVVYGSRFAGGESHRVLYFWHSVGNKMLTLASNMVTNLNLTDM
                     ETCYKTFRRELIQSVVIEEDRFGFEPEITAKLARSGARFYEVGISYDGRTYEEGKKIG
                     WRDGVQAIVCITKYGLLRRAEV"
     gene            complement(2593..3489)
                     /locus_tag="VLR27_18365"
     CDS             complement(2593..3489)
                     /locus_tag="VLR27_18365"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSP05475.1"
                     /translation="MDVLVTGGAGFIGSALVERLVAEGHHVEVVDDLTTGSLANLAAA
                     RDQARHEGAGALRIHQADVHDDGIAALVERRHPEVVIHLAGATAKSGRRATAQVVHEV
                     AGTVHVLDAAVAAGARKIVCVAGVRSQADVVRGIPARAVHEYLTAYEDRHGLAHTTVV
                     LPTVYGPRQTTATESSVVAVFVQRALAGQPCVVHGDGRQSRDLLYVDDAVDALVKAAL
                     HADGATVEVGTGVATTVSDLQVLVARVAGVECPQVPGERRVHEPGEVVTDPRRAATLL
                     GWEPWTTLEDGLAQTVAAARSA"
     gene            complement(3512..4504)
                     /locus_tag="VLR27_18370"
     CDS             complement(3512..4504)
                     /locus_tag="VLR27_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HSP05476.1"
                     /translation="MKAVILVGGFGTRLRPLTLTTPKNLLPVGHVPMVERVIGHLAKH
                     GVTEAVLSLGYKPDTFLDAYPDGTCAGVELHYAVEDEPLDTAGAIRFAAGEAGIDERF
                     VVVNGDVLTDLDVTRLIRFHDASGAEATIALHTVEDPSRYGVVVTDVAGQVEAFIEKP
                     PADEAPSNAINAGTYVLEPSVLGRIPVGRKVSVERETFPAIVADGSLYALDGNAYWLD
                     AGTPETYLQANLDLLLPDGASVHPEAFVADSAHVERSVVGAGASIGEDATLTEAVVLE
                     GATIEAGARVHRSIVGAGATVRAGAELLDLTVIGADVDIEPSARLTGDRVPADS"
     gene            complement(4501..5358)
                     /locus_tag="VLR27_18375"
     CDS             complement(4501..5358)
                     /locus_tag="VLR27_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05477.1"
                     /translation="MGKASSNKKVARAAKAGGGRARAAGERNILFPAALAAVVILGTL
                     LVVYAREERTAEALEAPLAFEDHWHSAYGIYVCDTFEPDLPEFTAPQNGGNHTHGDGL
                     FHIHPFSPARAGENATLVNWVTDAGAVLGGGDELSESRLGVPGGETYVEGEDSCEGVD
                     GDPIVQVAVWDSAFAASEGDDPDRVVTEDFGSIRFEDDGMAFTIAFAPEGAEIPAPPS
                     LDDLASVGSDLGVDPEDIPDDAELDEDFGEPAEGEPTDGADTEETDEEEGADESTTSS
                     TPTTADDGS"
     gene            complement(5371..6504)
                     /locus_tag="VLR27_18380"
     CDS             complement(5371..6504)
                     /locus_tag="VLR27_18380"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HSP05478.1"
                     /translation="MSVLRVGVNLCRIDPNELHHADHALLDSLGAVGADPPDDLQLVL
                     FASRSLIKARPALAAAFETHTFPFPPGAAPVRVAAEHTWLRLAVRRARLDLLHDAGGT
                     APGSVDVPRILSVHDLSPLERPRGVGWLRVAYHRRMVPRAVAQAAAVIVPSAFVRERL
                     VQTLHADESRISVVPWPLPPHGEAAPIETVKARRGIIGQIVLLPASTKPQEEHLVAVR
                     AMRHLAARHKETTLVLIGGEGPAERQVQAAIQELHLEERVVRLEGVSEAVRSALFENA
                     AVVVYPAVYGSFAHPVLEAMACGVPVVVADAGAAPELVADAGAVIAHGDDAQLAVEVH
                     RVLDDPQWRRSMVDAGLERARAYTPQRTAEGLLLAYRSVTAAL"
     gene            complement(6501..>7571)
                     /locus_tag="VLR27_18385"
     CDS             complement(6501..>7571)
                     /locus_tag="VLR27_18385"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HSP05479.1"
                     /translation="LEQCWAEVPGGTARTMIDLAGALDARADVEVVGVAARHDGPPPP
                     AWRPPVPVHHLPLPGHLLRESWHALRRPRIERATGPVDVCHAVGGAIPATAAPLVVTV
                     HDLAFVHFPELFSRQGRRFFHRALTLTRAHAVQVQVPSQATLEDCVAAGIERDRLRLV
                     PWGCTVTDPSPEDVAEAAARFGLPERYVVCVGTLEPRKNLPRLLQAWDRLARDDVELV
                     LIGPSGWGDARDLSTSGRNVTMTGFVDARTRDALYRGAVASCYPSLFEGFGLPVLESM
                     ALGCPVVTSSGTATAELAAGGAALTVEPTDVDAIAGALAEVLDDADLRARLAAAGSAR
                     AEEHTWERAAELVATGYEEVLA"
BASE COUNT         1040 a         2751 c         2775 g         1005 t
ORIGIN      
        1 ggcaagaact acgaggacca cgtcgtgatc gacgagaagt tctaccgccc ggccgaggtc
       61 gacctgctca tcggcgacgc caccaaggcc gagaagcagc tgggttggaa gcccaagacg
      121 tccttccccg agctggtggc gatgatggtc gatgccgaca tcgcgctgct caagggcgat
      181 ctgaagggca tctcgcactg agtgacaccg cggtcctctg cggcggggtg ggggcggcgc
      241 ggttcctgcg cggcctgctc gccgtcgtgc ccccgtcgtc ggtgaccgcg atcgtcaacg
      301 tcgccgacga caccgagctg cacgggctcc acatcagccc cgacatcgac acggtcacct
      361 acaccctcgc cgacgccatc gaccccgagc ggggttgggg gctgcgcgac gagacctggc
      421 acgccatgac gatgttcgag cgctacggca acccgtcgtg gttcaacctc ggcgacaagg
      481 acctggccac ccacatcgtg cgcaccgagc gcctgcgggc cggccaggcc ctgtccgagg
      541 tcaccgccca cctcgctcgg gcctgggacc tcgagtgcac gctgctaccc gtcaccgacg
      601 accggctccg gaccttcgtc accaccgagc acggcgagct cagcttccag gagtacttcg
      661 tgggtcgcca gcacgccgtc ccgatcagcg gggtgcgctt cgagggcgcc gacaccgccc
      721 gcccggcacc cggggtgctc gacgccctcg ccggcgccga ccgcatcgtg gtggcgccgt
      781 cgaaccccat cgtctccatc ggcccgctgc tcgccctcga cgaggtccgc gaggccctgg
      841 tgcgccaccg cgaccgggtg gtggccgtgt cgcccatcgt cgccggcgcc gccctgaagg
      901 gaccggccga tcgcatgctc accgagctcg gccacgagcc caccgtcgtc ggggtcgccc
      961 gcctctaccg cgacatcgcc gccacgctcg tcatcgacac cgccgatgcc gcgcacgccc
     1021 ctgcggtcga ggccgagggc atggcctgcg tcgtcaccgg caccgtcatg tccgacaccg
     1081 aacggtccgc cgccctggcc cgcgccgtcc tgaccgcgtc cggggccgag cgttgaccgt
     1141 ctcgccgccg ccggcgccgg gtgagctgcg catcctgccg gtgcacggca tcggggagat
     1201 ccgcccccgc gacgacctcg ccggcatcat cgccgacgcc tgcaacgccg tgccggccac
     1261 cgccctggtc gacggggacg tcctcgtcgt cacccagaag atcgtgtcca aggccgagga
     1321 ccgcatcgag gtcatcgaca ccgacgaccc gcgcgggcac aaggccatcg tcgagcgcga
     1381 gtcggtccgg atcctgcgcc ggcggggtga cctgatcatc gccgagacgt cgcacgggtt
     1441 cgtgtgcgcc aacgccggcg tcgacctgtc caacatcgag gccggctacg ccgccctgct
     1501 cccggtggac tccgaccggt cggcccgtcg catccgcgac gccatccgcc accgcctcgg
     1561 cgtcgaggtg gccgtcatcg tgtccgacac gttcgggcgc ccctggcggc gcggcctcac
     1621 cgacgtcgcc atcggctgcg ccgggatccg acccatcgcc gacctgcgcg gctccaccga
     1681 ctcgctcggc cgggagctgc aggtcaccga ggtctgcatc gtcgacgagc tcgccggtgc
     1741 cgccgacctc gtgtgcggca aggcctcggg cgtgcctgtg gcactgtgcc gtggcatcga
     1801 ccccgcctgg ctcggcgacg ggagcgtcgc cgacctcatc cgacacccca gcgaggacct
     1861 gttccggtga tccccctccg ccacgagccc cgcccctgcc tctcggtcgt gatgccggcc
     1921 tacaacgagc aggccaccgt cgccgctgcc atcgaggcgg tcctggcgtc gccctgggtc
     1981 gcccagctgg tcgtggtgga cgactgctcc accgacggca cgtccgccat cctcgacggc
     2041 atcaccgacg agcgggtcac caggctgcgc cacggcgtga atcagggcaa gggcgccgcc
     2101 ctgcgcaccg gcatcgccca cgccacctcc gagttcgtgg tcatccagga cgccgacctc
     2161 gagtacgacc cggccgagta cgccaagctg gtgcagccgc tgctcgacgg cagcgcggac
     2221 gtggtctacg ggtcccgctt cgccggcggg gagtcccacc gcgtcctgta cttctggcac
     2281 tcggtcggga acaagatgct cacgctcgcc tccaacatgg tgaccaacct gaacctcacc
     2341 gacatggaga cctgctacaa gacgttccgc cgcgagctga tccagtcggt ggtgatcgag
     2401 gaggaccgct tcgggttcga gcccgagatc accgccaagc tcgcccgcag cggcgcccgc
     2461 ttctacgagg tcggcatctc ctacgacggt cgcacgtacg aggagggcaa gaagatcggc
     2521 tggcgcgacg gcgtgcaggc catcgtctgc atcaccaagt acgggctcct ccgccgcgcc
     2581 gaggtctgac cgctaggccg atcgggccgc ggcgaccgtc tgggccagcc cgtcctcgag
     2641 ggtcgtccac ggctcccagc cgaggagggt ggcggcgcga cgtgggtcgg tgaccacctc
     2701 gcccggttcg tggaccctgc gctcccccgg cacctgcggg cactcgacgc cggccacccg
     2761 ggcgaccagc acctgcaggt cggacacggt ggtggcgacc ccggtgccga cctcgacggt
     2821 ggccccgtcg gcgtgcaggg cggccttcac gagggcgtcg acggcgtcgt cgacgtagag
     2881 caggtcgcgg gactggcggc cgtcgccgtg caccacgcag ggctggccgg ccagcgcccg
     2941 ctgcacgaag acggcgacca cgctggattc ggtggccgtc gtctggcgcg ggccgtagac
     3001 ggtggggagc acgaccgtgg tgtgggcgag gccgtggcgg tcctcgtagg ccgtcaggta
     3061 ctcgtgcacg gcccgggcgg ggatgccccg caccacgtcc gcctgcgagc gcacgccggc
     3121 gacgcacacg atcttgcggg cgccggcggc gacggcggcg tcgaggacgt gcacggtccc
     3181 ggccacctcg tgcaccacct gggcggtggc tcgccggccc gacttggcgg tggcgccggc
     3241 gaggtggatc acgacctctg ggtggcggcg ctcgacgagg gcggcgatgc cgtcgtcgtg
     3301 gacgtcggcc tggtggatgc gcagcgcccc cgcaccctcg tggcgggcct ggtcgcgcgc
     3361 cgcggccagg ttggcgagcg agccggtggt gaggtcgtcc acgacctcga cgtggtggcc
     3421 ctcggccacg agccgctcga cgagggccga gccgatgaac ccggcgccgc cggtcacgag
     3481 gacgtccaca ccagcccccg gtcgcgcccg gtcaggagtc cgccgggacc cggtcgccgg
     3541 tgaggcgggc agagggctcg atgtcgacgt cggcgccgat caccgtgagg tcgagcagct
     3601 cggcaccggc ccgcacggtg gcgccggccc cgacgatgga gcggtgcacg cgggcaccgg
     3661 cttcgatggt ggcgccctcg agcaccacgg cctcggtgag cgtggcgtcc tcgccgatgc
     3721 tggcgccggc cccgaccacg gaccgctcca cgtgggccga gtcggccacg aaggcctccg
     3781 ggtgcacgct ggcgccgtcg ggcaggagca ggtcgaggtt ggcctgcagg taggtctccg
     3841 gcgtgccggc gtcgagccag taggcgttgc cgtcgagggc gtagagcgag ccgtcggcca
     3901 cgatcgcggg gaacgtctcc cgttcgaccg agaccttgcg ccccaccggg atccgcccga
     3961 gcacgctcgg ctcgagcacg taggtaccgg cgttgatggc gttgctcggg gcctcgtcgg
     4021 ccgggggctt ctcgatgaag gcctcgacct ggccggccac gtcggtgacg accacgccgt
     4081 agcgggacgg gtcctcgacg gtgtggagag cgatggtggc ttcggcgccg gaggcgtcgt
     4141 ggaagcggat gagccgggtg acgtcgaggt cggtgagcac gtcgccgttc acgaccacga
     4201 agcgctcgtc gatgccggcc tcacccgcgg cgaagcggat ggcgccggcg gtgtcgaggg
     4261 gctcgtcctc gacggcgtag tgcagctcca ccccggcgca ggtgccgtcc gggtaggcgt
     4321 cgaggaacgt gtccggcttg tagccgagcg acagcaccgc ctcggtcacg ccgtgcttgg
     4381 ccaggtgccc gatgacccgc tcgaccatcg gcacgtggcc gacggggagc aggttcttgg
     4441 gcgtggtcag cgtgaggggt cggagacgcg tcccgaagcc accgaccagg atgacggcct
     4501 tcacgagccg tcgtcagccg tggtgggggt cgacgaggtg gtcgactcgt ccgcaccctc
     4561 ctcctcgtcg gtctcctcgg tgtccgcgcc gtcggtgggc tcgccctcgg ccggctcacc
     4621 gaagtcctcg tcgagctcgg cgtcgtcggg gatgtcctcg ggatcgacgc cgaggtcgga
     4681 gccgacgctg gccaggtcgt cgaggctggg cggtgccggg atctcggcgc cttcgggcgc
     4741 gaaggcgatg gtgaaggcca tgccgtcgtc ctcgaagcgg atggaaccga agtcctcggt
     4801 gacgacgcgg tcgggatcat cgccctcgga ggcggcgaac gcggagtccc acaccgcgac
     4861 ctgcacgatc gggtcgccgt cgacgccctc gcaggagtcc tcgccctcga cgtaggtctc
     4921 gccaccgggc acgccgaggc ggctctcgct gagctcgtcg ccgccgccga gcacggcgcc
     4981 ggcgtcggtg acccagttca cgagggtggc gttctcgccg gcgcgggccg gggagaacgg
     5041 gtggatgtgg aacaggccgt caccgtgggt gtggttgccg ccgttctggg gcgcggtgaa
     5101 ctcgggcaga tcgggctcga aggtgtcgca gacgtagatg ccgtaggcgg agtgccagtg
     5161 gtcctcgaag gccagcggcg cctcgagggc ctccgcggtg cgctcctccc gggcgtacac
     5221 gacgaggagc gtgcccagga tcaccacggc cgccagtgcg gccgggaaga ggatgttccg
     5281 ctcgcccgca gcgcgagcgc ggccgccacc ggccttcgca gcccgtgcaa ccttcttgtt
     5341 cgaggaggcc ttgcccatag cgaaacgacg ttacagggca gctgtcaccg aacggtaagc
     5401 gaggaggagt ccctcggcgg tccgctgggg ggtgtaggcc cgcgctcgct cgagcccggc
     5461 gtcgaccatc gagcgccgcc actgcggatc gtcgagcacc cggtggacct cgacggccag
     5521 ctgggcgtcg tcgccgtggg cgatcacggc accggcgtcg gcgacgagct ccggggccgc
     5581 accggcgtcg gccaccacga ccgggacacc gcaggccatg gcctcgagga ccgggtgggc
     5641 gaagctgccg tagacggcgg ggtacaccac gaccgcagcg ttctcgaaca gggcggatcg
     5701 gacggcttcg gacaccccct cgaggcggac gacacgctcc tcgaggtgca gctcctggat
     5761 cgcggcctgc acctggcgct cggccggccc ctccccgccg atcagcacca gcgtggtctc
     5821 cttgtggcgg gctgcgaggt ggcgcatcgc ccgcacggcg acgaggtgct cctcctgggg
     5881 cttggtgctc gccggaagca gcacgatctg gccgatgatc ccgcggcggg ccttgacggt
     5941 ctcgatgggt gccgcctcgc cgtggggcgg cagcggccag ggcaccaccg agatgcgcga
     6001 ctcatccgcg tgcagggtct ggaccaggcg ttcacggacg aacgccgagg gcacgatcac
     6061 cgccgcggcc tgggcgacgg cacgggggac catccggcgg tggtaggcga cccggagcca
     6121 gcccacgccg cggggtcgct cgagggggct gaggtcgtgc accgagagga tgcggggcac
     6181 gtcgaccgag ccgggtgcgg tgccaccggc gtcgtggagg aggtcgaggc gagcccggcg
     6241 cacggccagg cgcagccagg tgtgctcggc tgcgacccgg accggtgccg cccccggagg
     6301 gaacgggaac gtgtgggtct cgaacgccgc ggccagggcc gggcgggcct tgatgaggga
     6361 tcgggaggcg aacagcacga gctggaggtc gtcgggcggg tccgctccca cggcgccgag
     6421 cgagtcgagc agggcgtgat cagcgtggtg gagctcgttc ggatcgatcc ggcacaggtt
     6481 gaccccgacc cgcagcacgc tcatgccagc acctcctcgt agccggtggc gaccagctcg
     6541 gcggcccgct cccacgtgtg ctcctcggct cgtgccgagc cggcagcggc caggcgggcc
     6601 cgcaggtcgg cgtcgtccag cacctcggcg agggcaccgg cgatcgcatc gacgtcggtg
     6661 ggctccacgg tgagggcggc gccgccggcg gccagctcgg ccgtggccgt gcccgacgac
     6721 gtcacgaccg ggcagcccag cgccatcgac tccagcaccg ggaggccgaa gccctcgaac
     6781 aggctcggat agcacgacgc gaccgcgccg cggtacaggg catcacgggt tcgggcgtcc
     6841 acgaaccccg tcatcgtcac gttccgcccc gaagttgaca gatctctcgc gtcaccccaa
     6901 ccgctgggcc cgatcagcac cagctcgacg tcgtcgcggg ccagccggtc ccaggcctgc
     6961 agcaggcggg gcaggttctt gcggggctcc agcgtgccga cgcagaccac gtagcgctcc
     7021 gggagcccga agcgagcggc ggcctcggcg acgtcctcgg ggctcggatc ggtgacggtg
     7081 cacccccacg gcacgagccg caggcggtcc cgctcgatgc ccgccgccac gcagtcctcc
     7141 aaggtggcct gcgatggcac ctgcacctgc accgcgtgcg cccgggtcag ggtgagcgcc
     7201 cggtggaaga accgccgacc ctggcggctg aacagctcgg ggaagtggac gaaggccagg
     7261 tcgtggaccg tgaccaccag cggcgccgcc gtggcgggga tggcaccgcc gacggcgtgg
     7321 cagacgtcga ccggaccggt cgcccgctcg atccggggcc ggcggagggc gtgccagctc
     7381 tcgcgcagga ggtgcccggg caggggcagg tggtggaccg gcaccggggg acgccaggcc
     7441 gggggtggcg ggccgtcgtg gcgggcggcg acgccgacca cctccacgtc cgcccgggca
     7501 tcgagggcac cggccaggtc gatcatggtg cgcgccgtgc cacccggcac ctcggcccag
     7561 cactgctcca g
//
LOCUS       DATISU010000462         2855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_743663, whole genome shotgun sequence.
ACCESSION   DATISU010000462 DATISU010000000
VERSION     DATISU010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743663"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="VLR27_18390"
     CDS             complement(<1..1245)
                     /locus_tag="VLR27_18390"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HSP05480.1"
                     /translation="MSIATPTYVTERHRADLPNPILPEFWVRPADDIDADLAVLRAEG
                     PVFLPEPEIPEDIPLPQGPGAWAVTNHADILHISRHPDLFSSAQGIVVMDQPMQIVEY
                     FSSMIAMDDPRHARLRRIVSAGFTPRMLKRLEDSVQGLASQIVDDIIDRGECDFVVDV
                     AARLPLKIVCDLMGVPDSQLQFVFDRTNVILGVGDPEYTPEGTDILTALLTAGQELSA
                     LMDEVAEDKRGGDGTDLTSMLVNAELEDDRLTHGDLASFFILLVVAGNETTRNAIAWG
                     LHLLTQHPDQRAIWTEDFEGVAGTAVEEIVRIASPVTYMRRTATQDTELHGQVIPAGD
                     KLGMFYLAANRDEAVFDDPHRFDVRRTPNNHVGFGGPGPHFCLGAHLARREVSVVFRQ
                     LLARLPDSEVTGEVVPLEAAGIP"
     gene            complement(1242..>2855)
                     /locus_tag="VLR27_18395"
     CDS             complement(1242..>2855)
                     /locus_tag="VLR27_18395"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSP05481.1"
                     /translation="FAGLITGARLKEAGIDDVRIIEKGGDFGGTWYWNRYPGAQCDVE
                     SYIYLPLLEETGYIPKEKYTRAPEILEHCHRIARQYELYDNACLSTEVQAIDWDADRD
                     RWVIRTNRGDAMTARFLIMGNGPLHRPKLPGIPGVESFQGHTFHTSRWDYAYTGGDPD
                     GNLENLRDKRVGIIGTGATAVQCVPHLAESAQELFVFQRTPSSIDVRNNRPTDPEWAA
                     SLEPGWQKRRIENFTVLTSGGMADEDLVMDGWTDIIGKLLVRFRQSDNPDLTPSGIAS
                     TMELADFDKMNEIRSRVDELVHDQNTAEALKPWYRQFCKRPCFHDDYLQAFNRPNVHL
                     VDTDGQGVSRITETSVVVNGPDGEREYELDCLIYATGFEVGTAYTRRAGYDVTGARGQ
                     TLSDHWEGGMRSLHGMHVHGFPNMFLLGHAQGGFTANYPHLLEEASLQLVHVLRHAIE
                     NDVTQVEATEEAVEEWIQTLDEKARDMRSFQQSCTPGYYNNEGMPLQGVGLIGASYGE
                     GPMAFFRLMAEWRESGDMAGLELRHQGDR"
BASE COUNT          389 a          918 c         1066 g          482 t
ORIGIN      
        1 ggggatgccg gccgcctcca gcggcacgac ctcgccggtc acctcgctgt cggggagccg
       61 ggcgagcagc tggcggaaca ccacggagac ctcgcgacga gccaggtggg cgccgaggca
      121 gaagtgaggg ccggggccgc cgaagcccac gtggttgttc ggcgtgcgcc gcacgtcgaa
      181 gcggtgcggg tcgtcgaaca ccgcctcgtc gcggttggcc gccaggtaga acatgccgag
      241 cttgtcaccg gccgggatca cctggccgtg gagctcggtg tcctgggtgg cggtgcggcg
      301 catgtaggtg accgggctgg cgatgcgcac gatctcctcg accgcggtgc cggcgacgcc
      361 ctcgaagtcc tcggtccaga tggcgcgctg gtccgggtgc tgggtcagca ggtgcaggcc
      421 ccacgcgatg gcgtttcggg tggtctcgtt gccggcgacg acgaggagga tgaagaagct
      481 ggccaggtcg ccgtgggtga ggcggtcgtc ctcgagctcg gcgttgacca gcatgctggt
      541 gaggtcggtg ccgtcgccgc cccgcttgtc ctcggccacc tcgtccatga gcgccgacag
      601 ctcctggccg gcggtgagca gggcggtgag gatgtcggtg ccctcgggcg tgtactcggg
      661 atcgccgacg ccgaggatca cgttggtgcg gtcgaacacg aactgcagct ggctgtcggg
      721 cacccccatc aggtcgcaca cgatcttcag cggcaggcgg gcggcgacgt cgaccacgaa
      781 gtcgcactcg ccccggtcga tgatgtcgtc gacgatctgg gaggccagcc cctggaccga
      841 gtcctcgagc cgcttcagca tgcgtggcgt gaacccggcc gacacgatgc ggcgaagccg
      901 ggcgtggcgc gggtcgtcca tggcgatcat cgacgagaag tactcgacga tctgcatggg
      961 ctggtccatg acgacgatgc cctgcgccga ggagaagagg tccgggtgcc ggctgatgtg
     1021 gaggatgtcg gcgtggttgg tgaccgccca ggcccccggc ccctgcggga gcgggatgtc
     1081 ctcggggatc tccggctcgg gcaggaacac cggcccctcg gcgcggagca cggcgaggtc
     1141 ggcgtcgatg tcgtcggccg gtcgcaccca gaactcgggc aggatcgggt tgggcaggtc
     1201 cgcccgatgc cgttcggtga cgtacgtcgg cgtggcgatg ctcatcggtc cccctggtgg
     1261 cgcagctcga ggccggccat gtcgccggat tcccgccact ctgccatgag ccggaagaac
     1321 gccatcgggc cctccccgta gctggccccg atcaggccga cgccctgcag cggcatgccc
     1381 tcgttgttgt agtagccggg cgtgcacgac tgctggaacg agcgcatgtc gcgtgccttc
     1441 tcgtcgaggg tctggatcca ctcctcgacg gcttcctcgg tggcctcgac ctgggtcacg
     1501 tcgttctcga tggcgtggcg caggacgtgg accagctgga ggctggcctc ctcgagcagg
     1561 tgcgggtagt tggcggtgaa cccaccctgg gcgtgaccga gcaggaacat gttcgggaag
     1621 ccgtggacgt gcatgccgtg cagcgagcgc atgccgccct cccagtggtc cgagagggtc
     1681 tggccgcggg cgccggtgac gtcgtagccg gctcgccggg tgtaggccgt gccgacctcg
     1741 aacccggtgg cgtagatgag gcagtcgagc tcgtactcgc gctcgccgtc cggaccgttc
     1801 accaccacgc tcgtctcggt gatgcgcgag acgccctggc cgtcggtgtc gaccaggtgc
     1861 acgttcggcc ggttgaacgc ctgcaggtag tcgtcgtgga agcacggccg cttgcagaac
     1921 tggcggtacc agggcttgag ggcctcggcg gtgttctggt cgtgcaccag ctcgtcgacc
     1981 cgcgagcgga tctcgttcat cttgtcgaag tcggccagct ccatcgtcga ggcgatgccg
     2041 ctcggcgtga ggtccgggtt gtcgctctgg cggaaccgga cgagcagctt gccgatgatg
     2101 tcggtccagc cgtccatcac gaggtcctcg tcggccatcc cacccgaggt gagcaccgtg
     2161 aagttctcga tgcggcgctt ctgccaaccg ggctcgaggg acgccgccca ctccgggtcg
     2221 gtggggcggt tgttgcggac gtcgatggac gacggggtcc gctggaagac gaagagctcc
     2281 tgggccgact cggccaggtg gggcacgcac tgcaccgcgg tggcgccggt gccgatgatg
     2341 cccacccgct tgtcccgcag gttctcgagg ttcccgtccg gatccccgcc ggtgtaggcg
     2401 tagtcccacc ggctggtgtg gaacgtgtgc ccctggaagg actccacgcc ggggatgccg
     2461 ggcagcttcg gtcggtgcag cggcccgttg cccatgatca ggaaccgggc ggtcatggcg
     2521 tcgccgcggt tggtgcggat cacccagcgg tcgcggtcgg cgtcccagtc gatcgcctgc
     2581 acctcggtgg acaggcaggc gttgtcgtac agctcgtact ggcgggcgat gcggtggcag
     2641 tgctcgagga tctcgggcgc ccgggtgtac ttctccttgg ggatgtagcc ggtctcctcc
     2701 agcagcggga ggtagatgta ggactccacg tcgcactggg cgcccgggta gcggttccag
     2761 taccaggtgc cgccgaagtc gccgcccttc tcgatgatgc gcacgtcgtc gatgccggcc
     2821 tccttgaggc gggcgccggt gatcaggccg gcgaa
//
LOCUS       DATISU010000463         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_557753, whole genome shotgun sequence.
ACCESSION   DATISU010000463 DATISU010000000
VERSION     DATISU010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557753"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1658
                     /locus_tag="VLR27_18400"
     CDS             <1..1658
                     /locus_tag="VLR27_18400"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSP05482.1"
                     /translation="GFTTQRAQIVAIALLTFVVWALESLFQYLHQIAWRNLAQDLQHD
                     LRLDAYRNVQELEHAWFQERATGDLMAVLNDDVNQLERFLDIGANEIIQVLTTVVAVG
                     AIFFWLDPSVAMLAIAPMPLILWGSFWFQRRLEPRYEAVRNQAGFLNGQLANNLLGIS
                     TVKSFTAEDREAARIAGESDRYRQTNRRAISLSSAFVPLIRMAILVGFTGTMVWGGYE
                     AIKTATDGTGSLSVGQYSVLLFLSQRLLWPLTRLGETFDQYQRAMASTNRILDVVDTE
                     PAITGGDERLELDLGQGEVSFEDVTFAYEPGARPALDGVSLTIEAGRTTAVVGPTGSG
                     KSTLMRLLLRFYDVDEGRVCIEGHDVRDLDLGDLRHLTGLVSQDVYLFHGTVRENIAY
                     GRPDAPLEDVVAAATVAEIHDFVQTLPQGYDTIVGERGQKLSGGQRQRLSIARAVLKD
                     PVVLLLDEATSAVDNETEAAIQRSLERISAGRTTLVVAHRLSTVRNADVIHVLEAGRI
                     VESGTHDQLVTREGAYASLWAVQTGEAVHHDPHRAGPFRARPS"
     gene            complement(1639..>2849)
                     /locus_tag="VLR27_18405"
     CDS             complement(1639..>2849)
                     /locus_tag="VLR27_18405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSP05483.1"
                     /translation="QTRSVPAMPRRLLAFAAVSVLPTAVDVGLLVALREGLGWPLLVA
                     DLVAIAVASVVSYGAHRVATFRSNPYFRWVRQPLSFALLASAAAVVDALVLRATFTAF
                     GYTTVAGLLAAKAISLTVAAAVRAIGYRWVLGEAIREHRTPSGRPLEDGTCRLTVVIP
                     AYREPDRIGTTVRRIHDELADVAADGGLEVVVVDDGSQDDTAAHALAAGAEQVIVQPQ
                     NRGKGAAVRAGMLASRGRVALFTDADLAYDPPQLRRALEAVEDGWDVVIGNRRHVDSV
                     VSRATGLRALGSRVVNRLSAAVLLAAPRDTQCGLKAFRGDVARSLFAHARIDGFAFDI
                     EILHLVERAELSLGEIPVQLDESGSPSTVRVGHDVVRLALDLARVRRWSSIGAYDDVG
                     VVPTFTTDAP"
BASE COUNT          429 a         1070 c          985 g          365 t
ORIGIN      
        1 tcgggttcac cacgcaacgg gcccagatcg tcgccatcgc cctgctcacc ttcgtcgtct
       61 gggcgctcga gtcgctgttc cagtacctgc accagatcgc gtggcggaac ctggctcagg
      121 acctccagca cgacctgcgc ctcgacgcgt accgcaacgt gcaggagctg gagcacgcct
      181 ggttccagga gcgggccacc ggcgacctca tggccgtcct caacgacgac gtgaaccagc
      241 tcgagcggtt cctcgacatc ggcgccaacg agatcatcca ggtgctgacc accgtggtgg
      301 cggtcggcgc catcttcttc tggctcgacc cgtcggtggc catgctcgcc atcgccccca
      361 tgccgctgat cctgtggggg tcgttctggt tccagcggcg gctcgaaccg cgctacgagg
      421 cggtgcggaa ccaggccggg ttcctcaacg ggcagctggc caacaacctg ctcggcatct
      481 ccaccgtgaa gtccttcacc gccgaggacc gcgaggccgc ccgcatcgcc ggcgagagcg
      541 accgctaccg ccagaccaac cgccgggcca tctcgctgtc gtcggccttc gtcccgctca
      601 tccgcatggc gatcctggtc ggcttcaccg gcacgatggt ctggggcggc tacgaggcga
      661 tcaagaccgc caccgacggc accgggtcgc tgtcggtcgg ccagtacagc gtgctgctgt
      721 tcctgtccca gcgcctgctg tggcccctca cgcgcctggg tgagaccttc gaccagtacc
      781 agcgggccat ggcgtccacg aaccgcatcc tcgacgtcgt cgacaccgag ccggccatca
      841 ccggtggcga cgagcgcctc gagctcgacc tcggccaggg cgaggtgtcc ttcgaggacg
      901 tcacgttcgc ctacgaaccc ggcgcccgac cggcgctcga cggcgtgtcc ctgaccatcg
      961 aagcgggacg gaccacggcg gtggtcgggc cgaccggctc gggcaagtcc acgctcatgc
     1021 gcctcctgct gcgcttctac gacgtcgacg agggccgggt gtgcatcgag ggccacgatg
     1081 tccgcgacct cgacctcggc gacctgcgcc acctcaccgg cctggtcagc caggacgtgt
     1141 acctgttcca cggcacggtc cgggagaaca tcgcgtacgg acgccccgac gccccgctcg
     1201 aggacgtggt ggccgcggcg accgtcgccg agatccacga cttcgtccag accctccccc
     1261 agggctacga caccatcgtc ggcgaacggg gccagaagct ctccggcggc cagcgccagc
     1321 ggctctccat cgcccgggcg gtgctgaagg acccggtcgt gctgctgctc gacgaggcca
     1381 cctcggcggt cgacaacgag accgaggccg ccatccagcg ctcgctcgag cggatctcgg
     1441 ccgggcgcac caccctcgtc gtggcccacc ggctctcgac cgtgcgcaac gccgacgtga
     1501 tccacgtgct ggaggccggg cgcatcgtgg agtcgggcac ccacgaccag ctggtgaccc
     1561 gcgagggcgc ctacgcctcg ctgtgggccg tgcagaccgg cgaggccgtg caccacgacc
     1621 cccaccgggc gggtccgttc agggcgcgtc cgtcgtgaag gtcgggacca cgcccacgtc
     1681 gtcgtaggcc ccgatcgagg accaccgccg cacccgagcc aggtccaggg cgaggcgcac
     1741 gacgtcgtgg ccgacccgga ccgtggacgg cgagccggac tcgtcgagct gcaccgggat
     1801 ctccccgagc gacagctcgg cccgctccac caggtgcagg atctcgatgt cgaacgcgaa
     1861 cccgtcgatg cgggcgtggg cgaacagcga gcgggccacg tcgccgcgga acgccttcag
     1921 cccgcactgg gtgtcgcgcg gggcggcgag caggacggct gcggacagcc ggttgaccac
     1981 ccggctcccg agggcgcgca ggccggtggc ccgcgacacc acggagtcga cgtggcggcg
     2041 gttgccgatc acgacgtccc agccgtcctc caccgcctcg agcgcccgcc gcagctgggg
     2101 cgggtcgtag gccaggtcgg cgtcggtgaa cagcgccacc cgcccccgcg acgcgagcat
     2161 gccggcgcgc accgccgccc ccttgccgcg gttctgcggc tgcacgatga cctgctcggc
     2221 gccggcggcg agggcgtgcg cggccgtgtc gtcctgagac ccgtcgtcca cgaccaccac
     2281 ctcgagaccg ccgtcggcgg cgacgtcggc cagctcgtcg tggatgcgcc gcaccgtggt
     2341 cccgatccgg tcgggctccc ggtacgccgg gatcaccacg gtcaaccggc acgtgccgtc
     2401 ctccaggggc cgacccgacg gcgtgcggtg ctcgcggatg gcctcgccca gcacccagcg
     2461 gtacccgatg gcacggacgg cagccgccac ggtcaacgag atggccttgg cagcgagcag
     2521 gccggccacc gtcgtgtacc cgaaggcggt gaacgtggcc cgcagcacga gggcgtcgac
     2581 gacggcagcc gccgaggcca gcagggcgaa cgagagcggc tggcggaccc agcggaagta
     2641 cgggttggag cggaacgtgg ccacccggtg ggcgccgtag gagacgaccg aggcgacggc
     2701 gatggcgacg aggtcggcga cgagcagcgg ccagcccagc ccctcgcgca gcgccaccaa
     2761 caggccgacg tcgaccgcgg tcggcagcac cgacacggca gcgaaggcga gcaggcggcg
     2821 aggcatcgcc gggacgctac gcgtctgct
//
LOCUS       DATISU010000464         3169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_604328, whole genome shotgun sequence.
ACCESSION   DATISU010000464 DATISU010000000
VERSION     DATISU010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604328"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VLR27_18410"
     CDS             complement(<1..143)
                     /locus_tag="VLR27_18410"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF014332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HSP05484.1"
                     /translation="MMKSKIHRATVTDANLHYVGSVTVDEDLLDAADIMEFEQVAIVD
                     VDNG"
     gene            complement(161..1057)
                     /gene="panC"
                     /locus_tag="VLR27_18415"
     CDS             complement(161..1057)
                     /gene="panC"
                     /locus_tag="VLR27_18415"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HSP05485.1"
                     /translation="MRRIETVAELRTVLDAERAAGRRVGFVPTMGYLHEGHVSLMRAA
                     RADADVVVTSIFVNPLQFAAHEDLDDYPRDLERDASLAEDAGVDLLFVPSVAEMYPDP
                     VPGALLATTVSVAGVSEHMEGASRPTHFAGVATVVAKLFSIVGPCRAYFGEKDFQQLA
                     VVRRMVADLSMPVEVVGCETVREDDGLAMSSRNAYLSPEQRAVAPVVHRALRAGAAAV
                     LAGERDPGAVRAAIVGVVAQEPLAELDYAEIVSASDLTVPSTIDPSDALGLRLLAAVR
                     FGSTRLIDNLGVTAPAAPTVRS"
     gene            complement(1054..1782)
                     /locus_tag="VLR27_18420"
     CDS             complement(1054..1782)
                     /locus_tag="VLR27_18420"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HSP05486.1"
                     /translation="MTAVDARIIGPGRAGGALATALSDAGWRVDGPLGRDHDPATATT
                     GARVVLLAVPDRAVAGVAASLASGEAVVAHVAGSLGLDVLAPHQRVASVHPLVSLPDA
                     GIGARRLAGAWFAVAGDPVAAEIVDALGGRAVDVADEDRTTYHAAAAVAANHLVALLG
                     QVERLARQAGVPLEAYLDLARGALDNVAQVGPAAALTGPVARGDRATVARHLAAIPQD
                     ERATYAILAEAAQWLVDHAAEVPA"
     gene            complement(1828..2346)
                     /gene="folK"
                     /locus_tag="VLR27_18425"
     CDS             complement(1828..2346)
                     /gene="folK"
                     /locus_tag="VLR27_18425"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HSP05487.1"
                     /translation="MLLGLGSNLGDRRAHLRDAVSSLPGVTGVSDVYETDPVGGPEQD
                     RYLNIVVALDVPVVPGGGPPRPPEELSTDGTLPPITPLELLAICQRIESAAGRVRDVR
                     WGPRTLDVDVLWIDGVELDDPDLVVPHPRMFERRFVLAPLSELAPDILPEGWERDAEG
                     QVDHLGPLSSNP"
     gene            complement(2352..2720)
                     /gene="folB"
                     /locus_tag="VLR27_18430"
     CDS             complement(2352..2720)
                     /gene="folB"
                     /locus_tag="VLR27_18430"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HSP05488.1"
                     /translation="MSAGAIEIRGLRVLANVGVPEAERVHAQPLSIDLELTVDLDAAG
                     RSDDVADTVDYGAVAIAVADAVADAPVALLERVASLAADAVLDLDPRTEAVTVTITKL
                     RPPIPRDVEATAVRLHRTRP"
     gene            complement(2728..>3169)
                     /locus_tag="VLR27_18435"
     CDS             complement(2728..>3169)
                     /locus_tag="VLR27_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05489.1"
                     /translation="IIWLREQGFTMVVSLAVSPHNLHNYDEMGMPWRHRPFTRSDDQE
                     AYLSRLFPELRQLLLQGGKLVLHQDELGDRLSGLMAAYVLWNQLVPGGPQAISVVEQL
                     LHRQLGPDARSLVALVPRLPAPGTTTVSVDEPDPLPAVDPDEDA"
BASE COUNT          427 a         1063 c         1289 g          390 t
ORIGIN      
        1 ccgttgtcga cgtcgacgat cgcgacctgc tcgaactcca tgatgtcggc ggcgtcgagg
       61 aggtcctcgt cgacggtgac cgaccccacg tagtggaggt tcgcgtcggt gacggtggcg
      121 cggtggatct tggacttcat catgcgacgg cgcacgtgga tcagctcctc acggtgggcg
      181 ccgccggagc ggtcaccccc aggttgtcga tgagacgggt ggaaccgaag cgcacggcgg
      241 ccagcaggcg caaaccgagc gcatcggagg ggtcgatggt ggacgggacg gtgagatcgg
      301 aggccgagac gatctcggcg tagtcgagct cggcgagggg ttcctgggcc acgacgccga
      361 cgatggcggc acggacggcg ccggggtcgc gctcgccggc gaggacggcg gcggcgccgg
      421 cgcgcagggc ccggtgcacg accggtgcga cggcccgctg ctcgggcgag aggtaggcgt
      481 tgcggctgga catggccagg ccgtcgtcct cccgcacggt ctcgcagccg accacctcga
      541 ccggcatcga gaggtcggcg accatgcggc gcacgacggc gagctgctgg aagtccttct
      601 cgccgaagta cgcccggcac ggtcccacga tcgagaagag cttggccacg acggtggcga
      661 cgccggcgaa gtgggtgggc cgggaggcac cctccatgtg ctcggagaca ccggcgaccg
      721 agaccgtggt ggccagcagc gcgccgggca ccgggtcggg gtacatctcg gccaccgacg
      781 gcacgaacag caggtcgacg ccggcgtcct cggcgagcga cgcatcccgc tcgaggtcgc
      841 gggggtagtc gtcgaggtcc tcgtgcgccg cgaactggag ggggttcacg aagatcgacg
      901 tgacgacgac gtcggcgtcg gctcgggcgg ctcgcatcag cgagacgtgc ccttcgtgca
      961 ggtagcccat ggtcggcacg aacccgaccc ggcggccggc ggcccgttcg gcgtcgagca
     1021 cggtccgcag ctcggcgacg gtctcgatgc gcctcatgcc ggcacctcag cagcgtggtc
     1081 gaccagccac tgcgcggcct cggcgaggat ggcgtaggtg gcccgctcgt cctgggggat
     1141 ggcggccagg tgccgggcca cggtggcgcg gtcgccgcgg gcgaccggcc cggtcagcgc
     1201 cgcggccggt ccgacctggg cgacgttgtc gagcgcgcca cgagccaggt cgaggtaggc
     1261 ctcgaggggg acgccggcct ggcgggccag gcgctccacc tggccgagca gggcgacgag
     1321 gtggttggcg gcgacggcag cggcggcgtg gtaggtcgtg cggtcctcgt cggcgacgtc
     1381 cacggcccgt ccgccgagtg cgtccacgat ctcggccgcc accgggtccc cggccacggc
     1441 gaaccaggcg ccggcgaggc ggcgggcgcc gatcccggcg tcgggcagcg acacgagcgg
     1501 gtgcacggag gcgacccgct ggtgcggcgc cagcacgtcg aggccgagcg atccggcgac
     1561 gtgggcgacc accgcctcgc cggaggcgag cgaggcggcc acgccggcga cggcacggtc
     1621 ggggaccgcc agcagcacga cccgggcgcc ggtcgtcgcc gtggcgggat cgtggtcccg
     1681 accgagggga ccgtcgaccc gccagcccgc gtcggagagc gcggtggcga gggcgccgcc
     1741 ggcccgtccc gggccgatga tgcgcgcgtc gacagcagtc atgctcgctc cgatcgctca
     1801 gtggatccag ccccgtgcgg gtgccggtca ggggttggag gatagcggcc cgaggtggtc
     1861 gacctgcccc tcggcgtcgc gctcccaccc ctcggggagg atgtccggtg cgagctcgga
     1921 cagcggcgcg agcacgaagc ggcgctcgaa catgcggggg tggggcacga cgaggtcggg
     1981 gtcgtccagc tcgacgccgt cgatccagag cacgtcgacg tcgagcgtgc gcggccccca
     2041 gcggacgtcc cgcacccggc cggccgcgga ctcgatgcgc tggcagatgg cgagcagctc
     2101 cagcggggtg atcggcggga gcgtgccgtc ggtcgacagt tcctcgggtg ggcggggcgg
     2161 gccgccgccg ggcacgacgg gcacgtcgag ggcgaccacg atgttcaggt agcggtcctg
     2221 ctcggggccg cccaccgggt cggtctcgta cacgtcggac accccggtga cgccgggcag
     2281 cgaggacacg gcgtcgcgca ggtgcgcccg ccggtcgccg aggttggacc ccagcccgag
     2341 cagcacccgg gtcacggtcg ggtgcggtgc agccgcaccg cggtggcctc cacgtcgcgc
     2401 gggatgggag gtcggagctt ggtgatggtg acggtgaccg cctcggtccg ggggtcgagg
     2461 tcgagcaccg cgtcggcggc gagcgatgcg acccgctcga gcagcgcgac cggggcgtcg
     2521 gccaccgcgt cggcgacggc gatggcgacc gcgccgtagt cgacggtgtc ggccacgtcg
     2581 tcggagcgac cggcggcgtc gaggtcgacg gtgagctcca ggtcgatgga caacggctgg
     2641 gcgtggaccc gctcggcctc ggggacgccg acgttggcga ggacccgcag gccccggatc
     2701 tcgatggcgc ccgcgctcat cgcgtgctca cgcgtcctcg tcggggtcca ccgccggcag
     2761 cgggtcgggc tcgtcgaccg agacggtggt ggtgcccggg gcggggagcc gcgggaccag
     2821 cgcgacgagg ctgcgggcgt cggggccgag ctggcggtgg agcagctgct cgacgaccga
     2881 gatggcctgt gggccaccgg ggacgagctg gttccacagg acgtaggcgg ccatgaggcc
     2941 gctgagccgg tcgcccagct cgtcctggtg cagcacgagc ttgccgccct gcagcagcag
     3001 ctggcgcagt tcggggaaca ggcggctgag gtaggcctcc tggtcgtcgc tgcgggtgaa
     3061 ggggcggtgc cgccagggca tgcccatctc gtcgtagttg tgcaggttgt gcggggagac
     3121 ggccagcgac acgaccatgg tgaagccctg ctcgcgcagc cagatgatc
//
LOCUS       DATISU010000465         4756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_418371, whole genome shotgun sequence.
ACCESSION   DATISU010000465 DATISU010000000
VERSION     DATISU010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418371"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VLR27_18440"
     CDS             <1..496
                     /locus_tag="VLR27_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05490.1"
                     /translation="VGLRVTDDDTGADSDSTWVVVTGTDDRLRRPGWWYNQYDFSGRR
                     NKNNISEDTATCYLEAVRHMSRVFSAHRDCADFTEAMAILNTRQSSDENEIMVRQLLA
                     SWLNFAHGSVGWFDLVDTDGDKVADTPFHEVVAGAEALRLDPTATRAELLAYEDVLER
                     INGD"
     gene            complement(550..3348)
                     /locus_tag="VLR27_18445"
     CDS             complement(550..3348)
                     /locus_tag="VLR27_18445"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSP05491.1"
                     /translation="MSGQDAGLHGRDGERDRLTGMLHEAERGRGSTVVVSGPAGIGKT
                     RLTEFAMDYASAHRFNVRRCEFTLLADDPLGPVMGMLERADPLASARAEVLSSSVAER
                     TPGRSLFTITTPVAHTRLLDHLGRSAEALASRGPALLVVEDAHWGTLTTFAALAELQR
                     RASQGCLGLLVTTRTPVTPTTRQGHAALLEAGAAELPLGPLGDVAVERLVENLVGAPP
                     GASLRKSIDDTGGNPLFVSELVRGLSREGALSIVDGRVESEAGVVPSSLAGQVRRRLD
                     DLGPDARELLHAGAVLGSGFRVEELQLATGRSATSLLVPLDELLRSGLLEDTGVSLEF
                     RHELVRSVIYDDIPASVRLAVHRQVAETLQRSGASVHRVAAHYARGALAGDQDAVDAM
                     HVAARDLLGTASADALELLDRALEFEPSPRRRVELLLDRLEALAFGGRLDEAEALGRE
                     LLHVVEDPRRRLDLELDLGRILLISNRPSDAMEFFEAAAREPSARTMALAEASLAALV
                     AGDAIRSEQLADAAAADPEASPVARSLVLSMQSRAACHHLDLSRSRSLAEEAVRAADT
                     SEHLEGHRYQPHYFLCSTLFDLDLCEEAVRCAVTGRAVAERLGHSFGVPVYDAVEALI
                     HLKAGDLQRAMGAADEAIANCERTGSYLSLSLGEAVRALALVHLGRLDAARDAAQRCA
                     SALDENPPLLGSDIALLASALVKEAGGDPVGARDELAAATELFRSIGLPHMAAVFVVD
                     LARLAAATDQPSAGAVALEVVEDMASALDTPGWRGAALEVRGHLEREPALLAQAAERY
                     GRSPRRLDRARALEAAADLAADAGDGERSQPWYMRAIALYDACGASHDHDRAAAALRG
                     VGGSVPPPRVHPGGDPLSTREREIATLVRDGMTNADIADALYLSRRTVESHLRRIFHK
                     LELSSRVELALWLDANER"
     gene            3499..>4756
                     /gene="rpsA"
                     /locus_tag="VLR27_18450"
     CDS             3499..>4756
                     /gene="rpsA"
                     /locus_tag="VLR27_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HSP05492.1"
                     /translation="MTDTTSSATLVPAGLTESPSEMGTFNDEGEYTPRQITSNDVGSF
                     ADLIDDTMVTVEDGQLVEGKVVRIDKDEVLLDIGFKSEGVVPSRELSIRNDVDPSEVV
                     SLGDIVEALVLTKEDKEGRLILSKKRAQYERAWGKIEEIKEAEGVVTGPVIEVVKGGL
                     IIDIGLRGFLPASLVELRRVRDLAPYVGRSLEAKIIELDKNRNNVVLSRRAWLEETQR
                     EQREDFLQNLKPGEVRRGVVSSVVNFGAFVDLGGMDGLVHVSELSWKHVDHPGSVVAV
                     GDEIEVQVLEVDNDRERISLSLKATQQDPWQEFAGAHRVGELVYGRVTKLVPFGAFVQ
                     VGEGIEGLVHISEMSAHHVDLPEQVVTPGEELWVKIIDLDLQRRRISLSIKQAAEGGV
                     VAAEYQDAFGEHQYDDEGNFIGEVPED"
BASE COUNT          728 a         1707 c         1671 g          650 t
ORIGIN      
        1 ggtcgggctg cgggtgaccg acgacgacac gggtgccgac agcgacagca cgtgggtcgt
       61 ggtgaccggg accgacgacc ggttgcgccg gccgggctgg tggtacaacc agtacgactt
      121 ctcggggagg cgcaacaaga acaacatcag cgaggacacc gccacctgct acctggaggc
      181 ggtgcgccac atgagccggg tcttcagcgc ccaccgcgac tgcgccgact tcaccgaggc
      241 gatggccatc ctcaacaccc gccagagcag cgacgagaac gagatcatgg tccgccagct
      301 gctggcgagc tggctcaact tcgcccacgg ttcggtcggc tggttcgacc tcgtcgacac
      361 cgatggtgac aaggtcgccg acaccccgtt ccacgaggtc gtcgccggcg ccgaggcact
      421 ccgtctcgac ccgactgcga cccgggccga gctgctggcc tacgaggacg tgctcgagcg
      481 catcaacggc gactgaccag atcccgccgg ctcggatcgg taccacctcc ggtccgggcc
      541 ggtggacgct cagcgctcgt tcgcgtcgag ccagagggcg agctccaccc gtgaggacag
      601 ctcgagcttg tggaagatcc gccgcaggtg gctctcgacc gtgcgtcgcg acaggtacag
      661 cgcgtcggcg atgtcggcgt tggtcatgcc gtctcgcacc aacgtggcga tctcacgctc
      721 acgtgtgctc agcgggtcgc cccccggatg gacccggggc ggcggcaccg aacctccgac
      781 gcctcggagc gcagcggcgg cccgatcgtg gtcatgggat gcgccgcatg cgtcgtagag
      841 ggcgatggcg cgcatgtacc acggctgcga tcgctcgccg tcgccggcgt cggccgccag
      901 atcggcggcg gcctcgagcg cccgtgcccg gtccaggcgg cggggactgc gcccgtaccg
      961 ctcggccgcc tgggccagca gcgccggttc gcgctccagg tggccgcgca cctcgagggc
     1021 ggcccctcgc catccgggcg tgtcgagtgc gctcgccatg tcctcgacga cctcgagggc
     1081 gacggcaccg gcggacggtt ggtcggtcgc cgccgcgagt cgagccagat cgacgacgaa
     1141 gaccgctgcc atgtggggga ggccgatgga gcggaacagc tcggtggcgg ccgcgagttc
     1201 gtccctggcg ccgaccgggt cgcccccggc ctccttgacc agcgccgacg ccagcagcgc
     1261 gatgtcggac ccgagcagcg gtgggttctc gtcgagggcc gacgcgcacc gctgtgcggc
     1321 gtcgcgagcc gcatcgaggc gcccgaggtg gaccagcgcc aacgcgcgca cggcttcgcc
     1381 gaggctgagc gagaggtacg aaccggtccg ctcgcagttg gcgatcgctt cgtccgcggc
     1441 gcccatcgcc cgctggaggt ctcccgcctt gaggtggatc agcgcctcga ctgcgtcgta
     1501 cacgggcacg ccgaaggagt gcccgagccg ttcggccacg gcacgcccgg tgacggcgca
     1561 ccgcactgcc tcctcgcaca ggtcgaggtc gaagagggtc gagcacaaga agtagtgcgg
     1621 ctggtaccgg tgcccctcga ggtgctcgga cgtgtcggcg gcccggaccg cctcctcggc
     1681 cagggaacgg ctgcgggaga gatcgaggtg gtggcacgcg gctcgcgatt gcatcgagag
     1741 cacgagcgac cgggccaccg ggctcgcctc cgggtcggcg gcggccgcgt cggccagctg
     1801 ctcggatcgg atggcgtcgc ctgcgacgag cgcagcgagc gaggcctcgg cgagtgccat
     1861 cgtccgtgcc gacggctcgc gcgcggcggc ttcgaagaac tccatggcgt cgctcgggcg
     1921 gttcgagatg aggaggatcc ggccgagatc cagctccagg tcgaggcggc ggcgcgggtc
     1981 ctcgaccacg tggagcagct cgcggcccag cgcttcggcc tcgtcgagcc ggccgccgaa
     2041 agcgagcgcc tccagccggt cgagcagcag ctcgacgcga cgccggggcg acggttcgaa
     2101 ctccagcgcg cggtcgagca gctcgagggc gtccgccgac gccgtgccca gcaggtcacg
     2161 ggcggccacg tgcatcgcgt ccacggcgtc ctggtcgcca gcgagcgcac cgcgggcgta
     2221 gtgggcggcg acacggtgca ctgacgcccc gctccgctgc agggtctcgg cgacctggcg
     2281 gtgcacggcc agccggaccg atgcggggat gtcgtcgtag atcaccgagc ggacgagctc
     2341 gtgccggaac tcgagcgaca cgcccgtgtc ctcgaggagg ccggagcgga gcagctcgtc
     2401 cagcggcacc aacaggctgg ttgcgctgcg tcctgtcgcc agctggagct cctcgacgcg
     2461 aaacccggag ccgagcaccg ccccggcgtg gagcagctca cgtgcatcgg gacccaggtc
     2521 atcgaggcgg cggcgcacct gtccggcgag gctcgacggg accacccccg cctcggactc
     2581 gactcgtccg tcgacgatcg acaaggcgcc ctcgcggctg agcccccgta ccagctcgga
     2641 gacgaagagg gggttccccc cggtgtcgtc gatgctcttg cgcagcgagg cacccggcgg
     2701 ggcgccgacc aggttctcca ccagtcgttc gacggccacg tcaccgagcg gcccgagggg
     2761 cagctcggcc gctcctgcct cgagcagcgc cgcgtgcccc tgcctcgtcg tcggcgtgac
     2821 cggagtgcgt gtggtcacca ggaggcccag gcaaccctgg ctcgcccgcc gctggagctc
     2881 ggccagcgcg gcgaaggtgg tgagcgtccc ccagtgcgca tcctcgacga cgaggagcgc
     2941 cggcccccgg ctggcgagtg cctccgccga cctcccgagg tggtccagga gccgcgtgtg
     3001 cgccaccggc gtggtgatgg tgaacagcga gcgcccgggc gtccgctccg ccaccgagga
     3061 cgacaggacc tccgcccgtg ccgaagccag tgggtcggcc cgctcgagca tgcccatcac
     3121 cgggccgagc gggtcgtcgg cgagcaacgt gaactcgcag cgacgaacgt tgaaccggtg
     3181 cgctgacgcg tagtccatcg cgaactcggt gaggcgggtc ttgccgatgc ctgctgggcc
     3241 ggacacgacc accgtcgacc cgcgtccacg ctcggcctcg tggagcatcc cggtgaggcg
     3301 gtcccgctcg ccgtcgcgcc cgtgcagacc ggcgtcctgc ccgctcacgt gccagatcat
     3361 acgacccgtc gtcatctgca ggtccggcca caggctggtc gccccgcccg ggtcgctgct
     3421 acctttgctg attggcctct cgcgccctgc gaccaggcct tgtccggaaa acccaagcga
     3481 ccgagttaga agaacccttt gaccgacacc acgtccagcg ccaccctcgt ccccgcgggc
     3541 ctcaccgaga gccccagcga gatggggacc ttcaacgacg agggcgagta cacgccccgc
     3601 cagatcacct ccaacgacgt cggctccttc gccgacctca tcgacgacac gatggtcacc
     3661 gtcgaggacg gccagctcgt cgagggcaag gtcgtccgca tcgacaagga cgaggtcctg
     3721 ctggacatcg gcttcaagag cgagggcgtc gtgccctccc gtgagctgag catccgcaac
     3781 gacgtcgacc cgtccgaggt cgtctccctc ggcgacatcg tcgaggccct cgtcctcacc
     3841 aaggaggaca aggaaggccg cctgatcctg tccaagaagc gggcgcagta cgagcgtgcc
     3901 tggggcaaga tcgaggagat caaggaggcc gagggcgtcg tcaccggccc ggtcatcgag
     3961 gtcgtcaagg gcggtctcat catcgacatc ggcctgcgtg gcttcctgcc cgccagcctc
     4021 gtcgagctgc gccgtgtgcg cgacctggcc ccctacgtcg gtcgcagcct cgaggccaag
     4081 atcatcgagc tcgacaagaa ccgcaacaac gtggtcctgt cccgccgggc ctggctcgag
     4141 gagacccagc gcgagcagcg cgaggacttc ctccagaacc tcaagcccgg cgaggtccgc
     4201 cgtggcgtgg tctccagcgt cgtgaacttc ggcgccttcg tcgacctcgg cggcatggac
     4261 ggcctcgtgc acgtgtccga gctgtcctgg aagcacgtcg accaccccgg ctccgtcgtg
     4321 gccgtgggcg acgagatcga ggtccaggtc ctcgaggtcg acaacgaccg cgagcgcatc
     4381 agcctctccc tcaaggccac ccagcaggat ccgtggcagg agttcgccgg cgcccaccgc
     4441 gtgggcgagc tggtctacgg ccgggtcacc aagctggtcc ccttcggtgc gttcgtccag
     4501 gtgggcgagg gcatcgaggg tctggtccac atctcggaga tgtcggccca ccacgtcgac
     4561 ctccccgagc aggtcgtcac cccgggcgag gagctgtggg tcaagatcat cgacctcgac
     4621 ctccagcgcc gccgcatcag cctgtcgatc aagcaggccg ccgagggtgg cgtcgtggcc
     4681 gccgagtacc aggacgcctt cggcgagcac cagtacgacg acgagggcaa cttcatcggc
     4741 gaggtccccg aggacg
//
LOCUS       DATISU010000466        15591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_278185, whole genome shotgun sequence.
ACCESSION   DATISU010000466 DATISU010000000
VERSION     DATISU010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 15591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15591
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278185"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VLR27_18455"
     CDS             complement(<1..1025)
                     /locus_tag="VLR27_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HSP05493.1"
                     /translation="MTTESTAAPRFDIAQPEFWRRPLQERMDDFAAMRAQGPFWDSEI
                     PDTMGMLDGPVPFHAVTTYDEVVEISRKPKQFCSGQGAVAIPDMPAEALEFFGSFINM
                     DDPRHARQRGIVSRAFTPRRLESVLDSVQHICDEVIDDFCEKGEVDLVEAMSAPFPLL
                     VICDMMGIPRSEFKTVFDATNIILGGGDPEFVGDDIMGALFGAGMTLTGLLHEVAEDR
                     RKNPKDDLTTALITADVDDQMMTPEELGPFFILLAVAGNDTTRTAISYAMQFLTENPE
                     QRRIWQDDIEGVTPTAVEEVVRYASPVVFMRRTATEDVTLAGRDFSAGERFVMFYGAA
                     NRDPAHF"
     gene            complement(1077..1406)
                     /locus_tag="VLR27_18460"
     CDS             complement(1077..1406)
                     /locus_tag="VLR27_18460"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HSP05494.1"
                     /translation="MVLKRWRRDRDGGLPGGRGIPPAPTPPGEERPARRRREEPELVE
                     LVVGFDRFEASALVARLEASDIPVRLLTMDDHGLVVGMVARHPHRILIKADDEERVRA
                     IIERTAS"
     gene            complement(1431..1751)
                     /locus_tag="VLR27_18465"
     CDS             complement(1431..1751)
                     /locus_tag="VLR27_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HSP05495.1"
                     /translation="MAWPLLIVAGLLEVAWASVLPSTEGLTRLGPTLAFLGLLALSMV
                     ALAKAAEVIPIGTAYGVWVGVGAVGAAIVGIALHGDPATPARLFFLALLVTSIVGLKV
                     TGAS"
     gene            1907..2563
                     /locus_tag="VLR27_18470"
     CDS             1907..2563
                     /locus_tag="VLR27_18470"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HSP05496.1"
                     /translation="MLDDIVTWVTDVVDALGYLGVAFLVALENVFPPIPSEVVLPLAG
                     FVAGRGDANVVGMIVAATAGSVVGAWVLYGVSAAIGPMRLRAFIVRFGRWFSVREHHL
                     DRAEEWFDRRADAAVLIGRCVPLIRSVVSVPAGFRHMSPVRFTVLTAIGSAMWNTALI
                     AAGAVLGERWERVGDFVGLLQGAVILAIGGLLGWFVWRRFVRPRLAAAGDLPPEDHEG
                     "
     gene            complement(2571..3842)
                     /locus_tag="VLR27_18475"
     CDS             complement(2571..3842)
                     /locus_tag="VLR27_18475"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSP05497.1"
                     /translation="MSAERQRGLASAGRPVDDVIADLAAKRDGDVRWRDGRTFGLVFD
                     GGPGVHEAAERAAMLYLHENALNTMAFPSLGRIQSEVVGWTADLLHGPDTAAGFLTSG
                     GTESILCGVKAARERGRTERGITEPEMIVAESAHAAFHKAAHLFGITLHKTPVRDDWT
                     ADVGAMAEHVNANTVLIVGSAPPYPQGVMDPIPEIAALAASVDANCHVDACMGGFVLP
                     FAEMVGREVPPWDFRVEGVSSISADIHKLGYAPKGVSVILHRTKELRAHQTFVFDGWL
                     GGFYASPNLQGTRSGVPMAAAWAVMQHLGVDGYQALTRTVLDNADAMRAGIRAIDGLT
                     VLGDPTYHLLAIAPDPDAADPIDIFAVGDALQRRGWFHDRQSPPDNLHATVSNTNTGV
                     IEEYLAALRDSVDEVRGASADDRSTGYATLE"
     gene            complement(3839..4363)
                     /locus_tag="VLR27_18480"
     CDS             complement(3839..4363)
                     /locus_tag="VLR27_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05498.1"
                     /translation="MHRRLLAALAAGALLLSACGDDDSGNAGSGDDEYVEAMAASMQE
                     DEDLPFADEDIDCLSNEFVDALGGAERLEEEGITPEDLGSDEGLQDLGLELGQDEADG
                     IAASFGNCDVSLAELVLSEAGEDVPPDVRECVEENLDEDVLADFFATVLVDEETGEEP
                     PAELLEPLFTCFQS"
     gene            complement(4435..5175)
                     /locus_tag="VLR27_18485"
     CDS             complement(4435..5175)
                     /locus_tag="VLR27_18485"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSP05499.1"
                     /translation="MSGAEIAVVLGASLLGAFVKSVTGMGYPLLAVPLIALALGIEDA
                     VVIVSGPNAVANFLLCWQARGGRGDTRDLPVLVGTSFLGAFLGTFLLVTLPEDPLLIV
                     LAVTIVVFVVNFLRSPELRLREETTRRWSPVVGSLAGVMQGALGVSGPVVGAWMHGYR
                     LTSQAYVFSVTLIFGVAGSVQLVLLAASGRYTGERLGVSVLALIPVLLMIPVGMRSRT
                     RLGGRGFDLAVLGVLLASGAALVLRVLG"
     gene            5278..5856
                     /locus_tag="VLR27_18490"
     CDS             5278..5856
                     /locus_tag="VLR27_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05500.1"
                     /translation="MTARWVVDEVAPARDLQVTWQPISLFFKNEPDEGSEYYEPVWFT
                     HRLLRVLESVRAEGGDVEQLYLEYGRRIHHEQDRDFDVADALRAVGLDDAHAKAFDDD
                     RWDAEIRTRMDIGLALCGDDVGTPIIAMDDKHGKRAGYFGPVITRVPDTETSLELWDA
                     LRTMISVPGFWELKRTRTEGPEFGDPPTISGA"
     gene            complement(5843..7111)
                     /locus_tag="VLR27_18495"
     CDS             complement(5843..7111)
                     /locus_tag="VLR27_18495"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HSP05501.1"
                     /translation="MTAVDDQASDVQQQGDEVLANLFFSPEGIADPNPWYHRLRELAP
                     VHRSGTGTIFLTRFDDCNEVLRDNRFGNGDSDAGLGLPSADPATDEFRRDQRRRMADR
                     PQSLLFLDPPDHTRQRRLVSRAFTPRRVEQMRGTISDLARRYVDDLAEAGEGDLLDLV
                     GFPFPVSVIGALVGVPEADWPRFRSLITRGAVGIEPTATIDELRDGEQANVETWDYFV
                     DLVAERRARPQDDLLSDLIRVEEEGDQLSEAEVIVVTILIFAAGFETTTNLIGNGTGA
                     LLRNPAEMDRLWADEALLPTAVEEMLRWDSPVQFDVRRALRPAEVAGEAVDEGTAVVT
                     LLGAANRDPEHFTDPDRFDVGRDEGTPMSFASGIHYCLGANLARAEGQEVFRAMRDRF
                     RTIELAGDLVQRPRTTLRGYEAVPVRVTPR"
     gene            7135..7683
                     /locus_tag="VLR27_18500"
     CDS             7135..7683
                     /locus_tag="VLR27_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05502.1"
                     /translation="MTDGSVAAVERSTTWRSSERSRNGGLDRIPVPDAPGALWLCGKH
                     LIGPDVEAAITRVDADVVLCLNERHELEPRYPDYIAWLRTDPRAWWVPVPDLHAPPLP
                     DALALVETMAAHLEAGRTVLTHCGAGIGRAGTVAAAVLVARGATVEDAVAMVSASRPG
                     AGPEAGAQQALLEALGDRRNPE"
     gene            complement(7793..9469)
                     /locus_tag="VLR27_18505"
     CDS             complement(7793..9469)
                     /locus_tag="VLR27_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HSP05503.1"
                     /translation="MTPSPHDETAPSDFIREKIDADLAAGRFDRVQTRFPPEPNGYLH
                     IGHAKSICLNFGLAEQYGGPCKLRFDDTNPVAEDPEYVDAIIDDIRWLGFEPADVVHA
                     SDYFDQLYAWAEELVGKGMAYVDDQDAETISAQRGGFGEPGVESPFRDRTPEENLGLL
                     RQMRDGAFADGEKVLRAKIDMSASVMTMRDPVLYRIRRLPHHRTEDRWVIYPTYDWAH
                     GQSDAIEGVTHSLCTLEFADHRPLYDWCLEHLELPHEKPEQTEFARLGLTHTVMSKRL
                     LRTLVEDGVVDGWDDPRMPTIRGMRKRGYPPEAIRAFVDEIGVAKTNSTVEIELLESH
                     VRRHHNAHALRRMAVLDPVKVVITNWPEGQVEHVEAVNNPEDESAGTRQVPFSGELWI
                     ERDDFMADPPPKYYRLTPGREVRLRAGYFITATDAVTDADGNVTEVHATYDPETKGGQ
                     APDGRKVKATIHWVSAAHAEDLTVHLYERLFTDSHPGADGDAFDSLNASSRQTVQAKG
                     EPALADVAPGEVVQFERLGYFAADPDDPRTLHRTVGLRDEWANIQKRKGS"
     gene            9600..11234
                     /locus_tag="VLR27_18510"
     CDS             9600..11234
                     /locus_tag="VLR27_18510"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HSP05504.1"
                     /translation="MLPADLLDRLLPDTEPTSRWEEANPPRDLPAGAEVTRFAPSPTG
                     FLHIGGVYTATVAKELAERSGGSYFVRIEDTDQARAIEGSDEHFARAFPHFGIEPTEG
                     PEAAWGPYRQSERSEIYRSFVRALLESGHAYPDFVTKEELADITERQQTAKVDLGYWG
                     EWAVWRDAEPERVAAALDDGLPFTIRFRAPDAPGRVAFDDAIRGRIEADDNRNDVVLL
                     KSSAEPLPLPTYHLAHAVDDHLMRVTLVLRGDEWISSVPLHLQLFHALGFEPVRYAHV
                     APLMKGEGKSRRKLSKRKDPEASVDFWMAEGYPVGAVRHYLRGLANSRLADLPFAEAA
                     AEPLRLEEFGASGPLVDIPKLDDISADYVAEMPASDIRDRLLTWADEHDAELAGLLRA
                     KGDDLLAIIDVDRVDTDRVRKDIVKWSVFRERYGFFFPELFELVDDPGDERFLGVPPE
                     VIAAFATDFVAGYDADTASPGWFDQVRGLAERHGFALDRKAYKADPDAFHGTMREASN
                     IVRVTLTGSGQSPSLDQIAGVLGADEVRRRVGAPAG"
     gene            11240..12400
                     /locus_tag="VLR27_18515"
     CDS             11240..12400
                     /locus_tag="VLR27_18515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSP05505.1"
                     /translation="MDLSPDPDHEAIAEAVDAVCTRFDDAYWSDLDERHEFPWDFYRA
                     MAEGGWVGICIPEAYGGGGQGITEGALVLNRVAASGACMNGSTALHLTMFGLNPVVRF
                     GNDRLKDAFLPRAAVGDLHVAFGVTEPDAGTDTSRIATRAVDDGAGGFVVSGRKIWTS
                     KALESEVCLLLARTDGSPGTFDGLTLFLVDLDPAHCDIAPIPKVGRNAVASCEVAYDE
                     LPVEGWRMVGERGQGFRHILHGLNPERILLAAMACGIGEAALRRAVSYAGEREVFGRP
                     IGANQAIAHPLAQAHMQLTAAKAVMRDAAWRYDHDLPCGEQANTAKYLAAEASWFAAD
                     QAVQTHGGLGYATEYHVERYWREARLQRLAPVSQEMTCNFIAQNVLGLPRSY"
     gene            12660..13598
                     /locus_tag="VLR27_18520"
     CDS             12660..13598
                     /locus_tag="VLR27_18520"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HSP05506.1"
                     /translation="MAALAMFVAACGDDDDTDTSTDDTSADTDSDADSEDGEQASGSI
                     EAVEPFFPLDAEGNEVLPCGGYGFRLEDGDFYEAFNEQLNAFREDGTTMEIITGYEDF
                     SDADVEKANDLTAADIASNANAGMESGSGTLAELQEAGSITIGIANEVPFGFTGEDGE
                     ATGIAPDVAKAVLAELGITEVEANVVEFGQLIGGLQAGQYDLIAAGMYINDERAEQIL
                     FSDPDYCVAEGLAVPAGNPEGIVDYTSFVDNPDLTLAVATGTVEVGYAEDAGIPDDQL
                     QVFADIDSMYAALEAGEVDAVTGTAATVQRQVDARS"
     gene            13700..14437
                     /locus_tag="VLR27_18525"
     CDS             13700..14437
                     /locus_tag="VLR27_18525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HSP05507.1"
                     /translation="MDSWLLDSSQYELIWEGALVTLQILAYSFLLGLALSLVVGVARL
                     STRQWVRGVAFAYVEVARGISSIVLLFVIAIAVPILFEIEQQSLILLASIALGINMGG
                     YGAEIVRGSIQAIPKGQTEATIALNLSPTQRLRHVILPQAMRLILPPMGNLTIEILKG
                     TALVSLIGVTDIMQATRLINQQRLFTQSAADLTVLYVNVLVLYFVLSQIIAVLFRLAE
                     RRVESRYRGGDAEVPAAPSAPIPGVTK"
     gene            14434..15294
                     /gene="ehuD"
                     /locus_tag="VLR27_18530"
     CDS             14434..15294
                     /gene="ehuD"
                     /locus_tag="VLR27_18530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="HSP05508.1"
                     /translation="MTTTEPTTGAAEPTPSDEVQYRPHRSWYRRTDVVVPMILVALGY
                     LLLIVAVEEKSFFRTRLTAKGSENFDWMYFWHLVPLMWQGLQVLAKATVLGFTIAAVL
                     GLVLALGRRSGNRFVRWPFVAFIEFIRSTPLLVQLFFAQAYVRTSDSLSWSPITILVV
                     VLGIHYATYCSEAYRAGINSVPPGQWEAATALNLGPTTKWLRVILPQAIPNVLPSLGN
                     FLVAAFKDAPLGFIVGVPELLFFANTVRGSDFRPTEPYILAGVGFLLVSIPAAWAVRQ
                     LERKIAYERT"
     gene            15281..>15591
                     /locus_tag="VLR27_18535"
     CDS             15281..>15591
                     /locus_tag="VLR27_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSP05509.1"
                     /translation="MSAPETTASNPDISAKGAAAVRMRGVDKAFGANKVLAELDLDVE
                     PGERVVIIGPSGSGKTTILRVIMTLERPDAGTIQVGGRHLYHEVHGNGLKPASEKHIR
                     SV"
BASE COUNT         2241 a         5576 c         5496 g         2278 t
ORIGIN      
        1 tcgaagtggg ccgggtcgcg gttggcggcg ccgtagaaca tgacgaagcg ctcgccggcg
       61 gagaagtccc ggccggccag ggtgacgtcc tcggtggccg tgcggcgcat gaacaccacc
      121 ggagaggcgt agcgcacgac ctcctcgacc gccgtgggcg tgacgccctc gatgtcgtcc
      181 tgccagatgc ggcgctgctc ggggttctcg gtgaggaact gcatggcgta gctgatcgcc
      241 gtgcgggtgg tgtcgttgcc ggcgacggcc aggaggatga agaacggccc gagctcctcg
      301 ggcgtcatca tctggtcgtc gacgtcggcg gtgatgaggg cggtggtgag gtcgtccttg
      361 gggttcttgc gccggtcctc ggccacctcg tgcagcaggc cggtgagggt catgccggcg
      421 ccgaagaggg cgcccatgat gtcgtcgccc acgaactcgg ggtcaccgcc gccgaggatg
      481 atgttggtgg cgtcgaacac ggtcttgaac tccgaccgcg ggatgcccat catgtcgcag
      541 atcacgagca gggggaacgg ggcggacatg gcctcgacca ggtcgacctc gcccttctcg
      601 cagaagtcgt cgatgacctc gtcgcagatg tgctgcaccg agtcgagcac cgactcgagg
      661 cggcgggggg tgaacgcccg ggagacgatg ccgcgctgac gggcgtggcg ggggtcgtcc
      721 atgttgatga acgacccgaa gaactccagc gcctcagccg gcatgtcggg gatggccacc
      781 gcaccctggc ccgagcagaa ctgcttgggc ttgcggctga tctccacgac ctcgtcgtag
      841 gtggtgaccg cgtggaacgg caccgggccg tcgagcatcc ccatggtgtc ggggatctcg
      901 gagtcccaga acggaccctg ggcccgcatg gcggcgaagt cgtccatccg ctcctggagc
      961 ggtcggcgcc agaactccgg ctgggcgatg tcgaagcggg gggcggcggt cgactcggtc
     1021 gtcatgggcc gattctgctc actggcaacg tatgtgtcca agtgccgcga ggagcctcag
     1081 gacgccgtgc gctcgatgat cgcccgcacc cgctcctcgt cgtcggcctt gatgaggatg
     1141 cggtgcgggt gccgggcgac catccccacg accaggccgt ggtcgtccat cgtcaggagc
     1201 cggaccggga tgtcgctggc ctccagccgg gcgacgagcg cgctggcctc gaagcggtcg
     1261 aagcccacga cgagctcgac cagctcgggc tcctcgcggc gtcggcgggc cgggcgctcc
     1321 tcgcccggcg gggtcggcgc cggtgggatg ccccggccgc cggggagccc gccgtctcga
     1381 tcacgtcgcc accgcttgag gaccatgcgc catcgtcgcg caggcacgcc tcagctggcg
     1441 ccggtcacct tcagccccac gatggacgtg accagcaggg ccaggaagaa caggcgggcc
     1501 ggcgtcgccg ggtcgccgtg cagggcgatg cccacgatgg cggcgccgac cgcgcccacc
     1561 ccgacccaga cgccgtaggc ggtgccgatg gggatgacct cggcggcctt ggccagcgcc
     1621 accatgctga gggccaggag cccgaggaac gccagcgtcg gtccgagtcg ggtcagtccc
     1681 tcggtggagg gcagcaccga cgcccacgcc acctcgagca accccgcgac gatcagcagt
     1741 ggccaggcca tggaacgctc ctgagccccg tcttcgcgtt gccggtacgg gacaccctcg
     1801 tcggcggcgg agctgctccg cccagcgggc tccgacgata cccgcggtcg gtgggatcgg
     1861 catcaccccc acctacgctc accggacctg gccgagcgga ggaccgttgc tggacgacat
     1921 cgtcacctgg gtcaccgatg tggtcgacgc actgggctac ctcggcgtcg cgttcctcgt
     1981 cgccctcgag aacgtgttcc cgccgatccc ctccgaggtc gtcctgcccc tcgccggctt
     2041 cgtcgccggc cggggcgacg cgaacgtcgt cggcatgatc gtggccgcca ccgccgggtc
     2101 ggtcgtcggc gcctgggtcc tgtacggcgt gtcggcggcg atcggcccga tgcggctgcg
     2161 cgccttcatc gtccgcttcg gccgctggtt cagcgtgcgc gagcaccacc tcgaccgggc
     2221 cgaggagtgg ttcgaccggc gcgccgacgc ggcggtgctg atcggtcggt gcgtgccgct
     2281 gatccgctcg gtcgtgtccg tcccggcggg cttccgccac atgtcaccgg tccgcttcac
     2341 cgtcctcacc gccatcggca gcgcgatgtg gaacacggca ctgatcgccg ccggcgcggt
     2401 gctgggcgag cgctgggagc gggtgggcga cttcgtcggc ctgctccagg gcgcggtcat
     2461 cctcgccatc ggcgggctgc tcggctggtt cgtgtggcgg cgcttcgtcc gcccccgcct
     2521 cgccgccgcc ggcgacctgc cgcccgagga ccacgagggc tgagcggggc ctactcgagc
     2581 gtggcgtagc ccgtgctgcg atcgtcggcg ctggcgccgc gcacctcgtc caccgagtcg
     2641 cgcagcgcgg ccaggtactc ctcgatcacg ccggtgttgg tgttggacac ggtggcgtgg
     2701 aggttgtccg gcgggctctg gcggtcgtgg aaccaacccc gccgctgcag ggcgtcgccg
     2761 acggcgaaga tgtcgatcgg gtcggcggcg tcgggatcgg gggcgatggc cagcaggtgg
     2821 taggtggggt cgccgagcac ggtgagcccg tcgatcgccc ggatgccggc ccgcatcgcg
     2881 tcggcgttgt cgagcacggt gcgggtgagg gcctggtagc cgtcgacgcc gaggtgctgc
     2941 atgaccgccc acgccgcggc catcggcacg ccggagcggg tgccttggag gttcggggac
     3001 gcgtagaagc caccgagcca gccgtcgaag acgaacgtct ggtgagcgcg cagctccttg
     3061 gtgcggtgca ggatcaccga cacgcccttc ggcgcgtagc ccagcttgtg gatgtcagcc
     3121 gagatcgacg acaccccctc gacccggaag tcccacggcg gcacctcccg accgaccatc
     3181 tcggcgaagg gcaggacgaa cccgcccatg cacgcgtcga cgtggcagtt ggcgtcgacc
     3241 gaggcggcca gcgcggcgat ctcggggatg gggtccatga cgccctgggg gtacgggggc
     3301 gctgagccga cgatgagcac ggtgttggcg ttgacgtgct cggccatggc gccgacgtcg
     3361 gccgtccagt cgtcgcgcac cggggtcttg tgcagcgtga tcccgaacag gtgggcggcc
     3421 ttgtggaacg cggcgtgcgc gctctcggcc acgatcatct ccggctcggt gatcccccgc
     3481 tcggtgcggc cccgctcccg ggcggccttg acgccgcaga ggatggactc ggtgccgccg
     3541 ctggtgagga agccggccgc ggtgtcgggc ccgtggagga gatcggccgt ccagcccacg
     3601 acctcggact ggatccggcc gagggacggg aacgccatgg tgttgagggc gttctcgtgc
     3661 aggtagagca tggcggcgcg ctcggcggcc tcgtgcacgc ccgggccgcc gtcgaacacg
     3721 aggccgaagg tgcgcccgtc ccgccagcgc acgtcgccgt cgcgcttggc ggcgaggtcg
     3781 gcgatgacgt cgtcgacggg acgcccggca gaggcgaggc cgcgctggcg ttcggcgctc
     3841 atgactggaa gcaggtgaag agcggctcga gcagctcggc ggggggctcc tcgccggtct
     3901 cctcgtcgac gagcacggtg gcgaagaagt cggccaggac gtcctcgtcc aggttctcct
     3961 cgacgcactc gcggacgtcg gggggcacgt cctccccggc ctcggagagc accagctcgg
     4021 cgagggacac gtcgcagttg ccgaacgagg cggcgatgcc gtcggcctcg tcctggccca
     4081 gctccaagcc gaggtcctgg aggccctcgt cgctgccgag gtcctcgggg gtgatcccct
     4141 cctcctcgag gcgctcggcg ccgcccagcg catcgacgaa ctcgttggag aggcagtcga
     4201 tgtcctcgtc ggcgaagggc agatcctcgt cctcctgcat cgaagccgcc atggcctcca
     4261 cgtactcgtc gtcgccgctg ccggcgttgc cggagtcgtc gtcgccgcag gcgctgagca
     4321 gcagggcgcc ggcagcgagc gcggccagga gtcgtcggtg catcggatgc cttccctcgg
     4381 ggtcgtccgt cgccgcgccg tcgggggcgg gccggtcgac gaccctaacc gtgctcagcc
     4441 caggacccgc aggaccaggg cggcaccgga cgccagcagc accccgagca cggcgaggtc
     4501 gaacccccgt ccgccgaggc gggtccggga ccgcatgccg accgggatca tcagcagcac
     4561 cgggatcagg gccaggaccg agacccccag gcgctcgccg gtgtagcgac cgctggcggc
     4621 gagcagcacc agctgcaccg acccggcgac gccgaagatg agcgtcacgc tgaacacgta
     4681 ggcctgggag gtcagccggt agccgtgcat ccacgcaccc accaccggac cggacacgcc
     4741 gagcgccccc tgcatgaccc cggcgaggga gccgacgacc ggggaccagc gacgggtggt
     4801 ctcctcccgg aggcggagct cggggctgcg gaggaagttc acgacgaaca ccacgatcgt
     4861 cacggccagc acgatcagca gcgggtcctc cggcagggtc accagcagga aggtgccgag
     4921 gaacgcgccg aggaagcttg tgccgacgag caccggcagg tcccgcgtgt cgcctcgtcc
     4981 gcctcgggcc tgccagcaca gcaggaagtt cgccacggcg ttcgggcccg acacgatgac
     5041 caccgcgtcc tcgatgccga gggcgagcgc gatgagcggc acggccagca gcgggtagcc
     5101 catgccggtc accgacttca cgaaggcgcc gagcaggctg gcgccgagca ccacggcgat
     5161 ctcggccccg ctcacagcgg caggctaggg acggagggtg gcaacgaccg attcccacgg
     5221 catgctgcgc cgatggacat cgagttctgg atcgacccca tctgaccgtg gtgctgggtg
     5281 acggcccgtt gggtcgtcga cgaggttgca ccggcccgtg acctgcaggt cacgtggcag
     5341 ccgatcagct tgttcttcaa gaacgagccc gacgaggggt ccgagtacta cgagccggtg
     5401 tggttcaccc accggctgct ccgggtcctc gagtccgtgc gggccgaggg cggcgacgtc
     5461 gaacagctgt acctggagta cggccggcgc atccaccacg agcaggaccg cgacttcgac
     5521 gtcgccgacg cgctccgcgc cgtcggcctc gacgatgcgc acgccaaggc cttcgacgac
     5581 gatcgctggg atgccgagat ccgcacccgg atggacatcg gcctcgccct ctgcggcgac
     5641 gacgtcggca cgccgatcat cgccatggac gacaagcacg ggaagcgggc cggctacttc
     5701 ggtccggtga tcacccgggt gcccgacacc gagacctcac tcgagctgtg ggacgcgctg
     5761 cgcaccatga tcagcgtgcc cggcttctgg gagctcaagc gcacccgcac cgagggtccc
     5821 gagttcggcg acccgccgac gatcagcggg gcgtgacccg tacgggcacg gcctcgtagc
     5881 cgcgcagggt ggtgcggggg cgctgcacga ggtcgccggc gagctcgatg gtgcggaacc
     5941 ggtcgcgcat ggcccggaag acctcctggc cctcggcgcg cgccaggttg gcgccgaggc
     6001 agtagtggat gccggaggcg aacgacatcg gggtgccctc gtcccgaccg acgtcgaagc
     6061 ggtcggggtc ggtgaagtgc tcggggtcgc ggttggcggc gccgagcagg gtcaccaccg
     6121 cggtgccctc gtccaccgcc tcgccggcaa cctctgccgg gcggagggcg cggcgcacgt
     6181 cgaactggac cggtgagtcc cagcgcagca tctcctcgac ggcggtcggg aggagggcct
     6241 cgtcggccca gagccggtcc atctcggcgg ggttgcgcag cagcgccccg gtgccgttgc
     6301 cgatgaggtt cgtcgtggtc tcgaacccgg cggcgaagat gaggatcgtg acgacgatga
     6361 cctcggcctc gctgagctgg tcgccctcct cctcgacccg gatcaggtcc gacagcaggt
     6421 cgtcctgcgg acgggcccgg cgctcggcca ccaggtcgac gaagtagtcc cacgtctcga
     6481 cgttggcctg ctcgccgtcc cgcagctcgt cgatggtggc ggtgggctcg atgccgacgg
     6541 caccgcgggt gatcagcgac cggaaccggg gccagtccgc ctcgggcacc ccgacgagag
     6601 caccgatgac cgacacgggg aacgggaagc cgacgaggtc gagcaggtcg ccctcccccg
     6661 cctcggcgag gtcgtccacg tagcggcgag cgaggtcgga gatcgtcccg cgcatctgct
     6721 cgacccgacg gggggtgaag gcacgggaga cgaggcgccg ctggcgggtg tggtccggcg
     6781 ggtcgaggaa cagcagcgac tgggggcggt cggccatgcg gcggcgctgg tcccggcgga
     6841 actcgtcggt ggccgggtcg gcgctcggca gacccaggcc ggcgtcggag tcgccgttgc
     6901 cgaaccggtt gtcccggagc acctcgttgc agtcgtcgaa gcgggtgagg aagatcgtgc
     6961 cggtcccgct gcggtggacc ggggccagct cccgcaggcg gtggtaccag gggttcggat
     7021 cggcgatgcc ctcggggctg aagaagaggt tggccagcac ctcgtcgccc tgctgctgca
     7081 cgtcgctggc ctggtcgtcc actgcggtca tcggtcgtcc ccctcggtcg ttcggtgacc
     7141 gacggtagcg tcgccgcggt ggagcggtcg acgacgtggc gcagcagcga gcgctcccgc
     7201 aacggcggcc tcgaccggat ccccgtcccc gatgcccccg gcgccctctg gctctgcggc
     7261 aagcacctca tcggacccga cgtcgaagcc gccatcaccc gggtcgacgc cgacgtggtc
     7321 ctgtgcctca acgagcgcca cgagctcgag ccccgctacc ccgactacat cgcctggctg
     7381 cgcaccgacc cgagggcctg gtgggtcccc gtccccgacc tgcacgcgcc cccgctcccc
     7441 gacgcgctcg ccctcgtcga gaccatggca gcccacctcg aagccggccg caccgtcctc
     7501 acccactgcg gcgccggcat cggccgagcc ggcacggtgg cagcagcagt gctcgtcgcc
     7561 agaggagcga ccgtcgagga cgccgtggca atggtgtcgg ccagccgccc cggcgcaggc
     7621 ccagaggccg gcgcccaaca agccctgctc gaagccctgg gcgacaggcg caacccggaa
     7681 tgaaactggc agccgaagcg tcacgcagcc ccgcaggggg ttgtgtgacg aggcggctgc
     7741 cagtttccag agtcggaaca caagaggcgg ccgaagatgc cgccagcaac cgctacgagc
     7801 ccttccgctt ctggatgttg gcccactcgt cccgaagccc gacggtgcgg tgcagcgtgc
     7861 gggggtcgtc ggggtcggcg gcgaagtagc cgaggcgctc gaactggacg acctcgccgg
     7921 gggcgacgtc ggcgagcgcc ggttcgccct tggcctgcac ggtctggcgc gacgaggcgt
     7981 tgagcgagtc gaaggcgtcg ccgtcggcac cggggtggct gtcggtgaac agccgctcgt
     8041 agaggtggac ggtgaggtcc tcggcgtggg cggcggagac ccagtggatg gtggccttga
     8101 ccttgcgtcc gtcgggggcc tggccgccct tggtctcggg gtcgtaggtg gcgtggacct
     8161 cggtgacgtt gccgtcggcg tcggtgaccg cgtcggtggc ggtgatgaag tagccggccc
     8221 gcaggcggac ctcccggccc ggggtgaggc ggtagtactt cggcggcggg tcggccatga
     8281 agtcgtcccg ctcgatccac agctcgccgc tgaagggcac ctgccgggtg ccggcggact
     8341 cgtcctcggg gttgttgacc gcctccacgt gctcgacctg gccctcgggc cagttggtga
     8401 tgaccacctt caccgggtcg agcacggcca tgcggcgcag ggcgtgggcg ttgtggtgcc
     8461 ggcgcacgtg ggactcgagc agctcgatct cgacggtgga gttggtcttg gccacgccga
     8521 tctcgtcgac gaaggcgcgg atggcctcgg gcgggtagcc ccgcttgcgc atgccgcgga
     8581 tggtgggcat gcgggggtcg tcccacccgt cgaccacacc gtcctccacc agcgtgcgca
     8641 gcagtcgctt ggacatgacg gtgtgggtga ggccgagtcg ggcgaactcg gtctgctcgg
     8701 gcttctcgtg gggcagctcg aggtgctcga ggcaccagtc gtagagcggc cggtggtcgg
     8761 cgaactcgag cgtgcacagc gagtgggtga cgccctcgat ggcgtcggac tggccgtggg
     8821 cccagtcgta ggtcgggtag atgacccagc ggtcctcggt gcggtggtgg ggcaggcgac
     8881 ggatccggta cagcaccggg tcgcgcatcg tcatgacgga cgccgacatg tcgatcttgg
     8941 cccgcagcac cttctcaccg tcggcgaacg cgccgtcgcg catctggcgg agcaggccga
     9001 ggttctcctc cggcgtacgg tcccggaacg ggctctcgac gcccggttcc ccgaagccgc
     9061 cgcgctgggc cgagatggtc tcggcgtcct ggtcgtccac gtaggccatc cccttgccga
     9121 cgagctcctc ggcccaggcg tacagctggt cgaagtagtc gctggcgtgc acgacgtcgg
     9181 cgggctcgaa gccgagccac cggatgtcgt cgatgatcgc gtcgacgtac tcggggtcct
     9241 cggcgacggg gttggtgtcg tcgaagcgca gcttgcacgg gccgccgtac tgctcggcca
     9301 ggccgaagtt gaggcagatc gacttggcgt ggccgatgtg gaggtagccg ttgggctccg
     9361 gtgggaagcg ggtctgcacc cggtcgaagc gacccgcggc caggtcggcg tcgatcttct
     9421 cccggatgaa gtcgctggga gcggtctcgt cgtgggggga cggggtcacc ctccaatgct
     9481 ggcacctcag gagccgagcg cagcgaagcg acccatgagc acgggcattc cggcggcgga
     9541 gccgccaacc tggtcgcaag tgggtgcacc ccgtccgcgc acgtatcgtc ggccccacca
     9601 tgcttcccgc cgacctcctc gatcgactgc tgcccgacac ggagcccacc tcgcgctggg
     9661 aggaggccaa cccgccccgc gacctgcctg cgggcgccga ggtcacccgg ttcgccccca
     9721 gccccaccgg cttcctccac atcggtggcg tgtacacggc gacggtggcc aaggagctgg
     9781 ccgagcgcag cggcggcagc tacttcgtcc gcatcgagga caccgaccag gcccgcgcca
     9841 tcgagggctc cgacgagcac ttcgcccgtg ccttcccgca cttcggcatc gagcccaccg
     9901 agggacccga ggcggcctgg ggcccgtacc gccagtccga gcggtccgag atctaccgca
     9961 gcttcgtccg ggcgctgctc gagtccggcc acgcctaccc ggacttcgtc accaaggagg
    10021 agctggccga catcaccgag cgccagcaga cggccaaggt cgacctcggc tactggggcg
    10081 agtgggccgt gtggcgcgac gccgagcccg agcgggtcgc cgccgcgctc gacgacggcc
    10141 tccccttcac catccgcttc cgggctccgg atgcacccgg ccgggtcgcg ttcgacgacg
    10201 ccatccgggg ccgcatcgag gccgacgaca accgcaacga cgtggtcctc ctcaagagct
    10261 cggccgagcc actccccctc cccacctacc acctcgccca cgcggtcgac gaccacctga
    10321 tgcgggtcac actcgtgctc cgcggcgacg agtggatctc ctcggtgccg ctgcacctgc
    10381 agctgttcca cgccctcggc ttcgagccgg tgcgctacgc ccacgtcgcc cccctcatga
    10441 agggcgaggg caagagccgg cgcaagctgt ccaagcgcaa ggaccccgag gcgtcggtcg
    10501 acttctggat ggccgagggc tacccggtcg gcgcagtccg tcactacctg cggggcctgg
    10561 ccaactcccg cctcgccgac ctgcccttcg ccgaggccgc cgccgagccc ctccggctcg
    10621 aggagttcgg cgcctccgga ccgctcgtcg acatccccaa gctcgacgac atctccgccg
    10681 actacgtggc cgagatgccg gcctcggaca tccgggaccg gttgctgacc tgggccgacg
    10741 agcacgacgc cgagctggcc gggctgctgc gagccaaggg cgacgacctg ctcgccatca
    10801 tcgacgtcga ccgcgtcgac accgaccggg tccgcaagga catcgtgaag tggtcggtgt
    10861 tccgcgagcg gtacggcttc ttcttccccg agctgttcga gctggtcgac gaccccggcg
    10921 acgagcgctt cctgggggtg ccgcccgagg tgatcgctgc gttcgccacc gacttcgtgg
    10981 ccggctacga cgccgacacc gcctcccccg gatggttcga tcaggtgcgc ggcctggccg
    11041 agcgccacgg cttcgccctc gaccgcaagg cgtacaaggc cgaccccgac gccttccacg
    11101 gcaccatgcg cgaggcgtcc aacatcgtgc gggtcaccct caccggctcg ggccagagcc
    11161 cgagcctcga ccagatcgcc ggggtgctcg gcgccgacga ggtccgccgc cgcgtcggcg
    11221 cgccggccgg ctgacgccga tggacctcag ccccgacccc gatcacgagg cgatcgccga
    11281 ggccgtcgac gccgtctgca cccgcttcga cgacgcctac tggtcggacc tggacgagcg
    11341 ccacgagttc ccgtgggact tctaccgggc catggccgag ggcggctggg tcggcatctg
    11401 catccccgag gcctacggcg gaggcggcca gggcatcacc gagggtgcgc tcgtgctgaa
    11461 ccgggtggcg gcgtccggcg cgtgcatgaa cggttccacc gccctgcacc tgaccatgtt
    11521 cggcctgaac ccggtggtgc gcttcggcaa cgaccggctc aaggacgcgt tcctcccccg
    11581 cgccgcggtc ggcgacctgc acgtcgcgtt cggcgtcacc gagcccgacg ccggcaccga
    11641 caccagccgc atcgccaccc gggccgtcga cgacggcgcc ggcggcttcg tcgtgtccgg
    11701 ccgcaagatc tggacgtcga aggcgctcga gtccgaggtg tgcctgctgc tcgcccgcac
    11761 cgacggcagc cccggcacct tcgacggcct cacgctgttc ctcgtcgacc tcgacccggc
    11821 ccactgcgac atcgccccca tccccaaggt gggtcgcaac gccgtcgcct cctgcgaggt
    11881 cgcctacgac gagctccccg tcgagggctg gcgcatggtg ggcgagcgcg gccaggggtt
    11941 ccgccacatc ctccacggcc tcaacccgga gcggatcctg ctggccgcca tggcgtgcgg
    12001 catcggtgag gcggcgctgc gccgagccgt gtcctacgcc ggcgagcgtg aggtcttcgg
    12061 ccgacccatc ggcgccaacc aggccatcgc ccacccgctc gcccaggccc acatgcagct
    12121 caccgcggcg aaggccgtca tgcgcgatgc ggcgtggcgc tacgaccacg acctgccgtg
    12181 cggcgagcag gccaacacgg ccaagtacct cgccgccgag gccagctggt tcgccgccga
    12241 ccaggcggtg cagacccacg gcggtctcgg ctacgccacc gagtaccacg tggagcgcta
    12301 ctggcgcgag gcacgactcc agcggctggc gccggtgagc caggagatga cgtgcaactt
    12361 catcgcccag aacgtcctgg ggctgccccg gagctactga agcccgccgc tgctttcact
    12421 gcggttgtgt tgcgatccgt ggcagatcgg aaatcccgtg ctaggggttg ttgccgtccc
    12481 gggccgtcgc ggcacgtctg cgttcggtgt cgagtcgagt cactggtctc cttcgggagc
    12541 gatcggtggc ccgcctcggc gcaggccgtc cggggacaca caacccaacg aacggagcga
    12601 agcagatgcc tgcctcaaag tcccgaactc gtgccccctg gctacgactc atcgcactca
    12661 tggccgccct ggccatgttc gtcgccgcct gcggcgatga cgacgacacc gacaccagca
    12721 ccgacgacac gtcggccgac acggacagcg acgccgacag cgaggacggc gagcaggcct
    12781 cgggcagcat cgaggccgtc gagcccttct tcccgctcga cgccgagggc aacgaggtcc
    12841 tcccctgcgg tggctacggc ttccggctcg aggacggcga cttctacgaa gccttcaacg
    12901 agcagctcaa cgccttccgt gaggacggca ccacgatgga gatcatcacg gggtacgagg
    12961 acttctccga cgccgacgtc gagaaggcca acgacctgac cgctgccgac atcgcctcca
    13021 acgccaacgc gggcatggag tccggctcgg gcacgctcgc cgagctgcag gaagccggtt
    13081 ccatcaccat cggcatcgcc aacgaggtgc ccttcggctt caccggtgaa gacggcgagg
    13141 ccaccggcat cgcgcccgac gtggccaagg ccgtgctcgc cgagctcggc atcaccgagg
    13201 tcgaggccaa cgtggtcgag ttcggtcagc tcatcggcgg cctgcaggcc ggccagtacg
    13261 acctgatcgc tgcgggcatg tacatcaacg acgagcgggc cgagcagatc ctgttctccg
    13321 atccggacta ctgcgtggcc gagggcctcg ccgtcccggc gggcaacccc gagggcatcg
    13381 tcgactacac gtccttcgtc gacaacccgg acctgaccct cgctgtcgcc accggcaccg
    13441 tcgaggtcgg ctacgccgag gacgccggca tccccgacga ccagctccag gtcttcgcgg
    13501 acatcgactc gatgtacgcc gcgctcgagg ccggtgaggt cgacgccgtg accggcacgg
    13561 ccgccaccgt ccagcgccag gtggacgccc ggtcctgatc cgatcggaac acgccgaccc
    13621 ggatcgagcg gtgcttgacg gcgccgctcg gtccgggtcg actcatgctt gaccgcagag
    13681 ctgcgaaggc gaggtcgcga tggacagctg gttgctcgat tcgagccagt acgagctgat
    13741 ctgggaaggg gcgctggtca ccctccagat cctggcgtac tcgttcctgc tcggcctggc
    13801 gctgtcgctc gtggtgggcg tcgcccgcct ctccacccgc cagtgggtgc gcggggtcgc
    13861 cttcgcctac gtggaggtcg cccgcggcat ctcctcgatc gtgctgctgt tcgtcatcgc
    13921 catcgcggtg ccgatcctgt tcgagatcga gcagcagtcc ctgatcctgc tggcctcgat
    13981 cgccctcggc atcaacatgg gcggctacgg cgcggagatc gtccgcggct cgatccaggc
    14041 catccccaag ggccagaccg aggccaccat cgcgctgaac ctgagcccca cccagcggct
    14101 gcgccacgtg atcctgcccc aggccatgcg gctcatcctg ccgccgatgg gcaacctcac
    14161 catcgagatc ctcaagggga cggcgctggt cagcctgatc ggcgtcaccg acatcatgca
    14221 ggcgacgcgc ctcatcaacc agcagcggtt gttcacccag tcggccgccg acctcaccgt
    14281 gctgtacgtg aacgtgctgg tcctctactt cgtgctgtcc cagatcatcg ccgtgctctt
    14341 ccgcctcgcc gagcggcggg tggagagccg ctaccggggc ggtgacgccg aggtgcccgc
    14401 cgcaccgagc gcgccgatcc cgggggtgac caagtgacca ccaccgaacc cacgaccggc
    14461 gccgcagagc ccacgccgag cgacgaggtc cagtaccggc cccaccggtc ctggtaccgc
    14521 cgcaccgacg tcgtggtgcc gatgatcctc gtcgccctcg gctacctcct gctcatcgtg
    14581 gccgtggagg agaagtcgtt cttccgcacc cggctcacgg cgaagggcag cgagaacttc
    14641 gactggatgt acttctggca cctggtgccg ctcatgtggc agggcctcca ggtgctggcc
    14701 aaggccacgg tgctcggctt caccatcgcc gccgtcctcg gcctcgtcct cgccctcggg
    14761 cgccgctcgg ggaaccggtt cgtgcgctgg cccttcgtcg cgttcatcga gttcatccgg
    14821 tccaccccgc tgctcgtgca gctgttcttc gcgcaggcgt acgtgcggac ctcggactcc
    14881 ctgtcctggt ctccgatcac gatcctggtg gtcgtcctgg gcatccacta cgccacctac
    14941 tgctccgagg cgtaccgggc cggcatcaac agcgtgccgc ccggccagtg ggaggcggcc
    15001 accgcgctca acctgggccc gaccaccaag tggctgcggg tgatcctgcc ccaggccatc
    15061 cccaacgtct tgccgtccct cggcaacttc ctggtggcgg cgttcaagga cgcgcccctc
    15121 gggttcatcg tgggcgtgcc cgagctgctg ttcttcgcca acaccgtgcg gggctccgac
    15181 ttccgcccca ccgagcccta catcctcgcc ggcgtggggt tcctgctggt gagcatcccg
    15241 gccgcctggg cggtccgcca actcgagagg aagatcgcgt atgagcgcac ctgagaccac
    15301 ggcctcgaac ccggacatct ccgccaaggg ggccgctgcc gtccggatgc gcggcgtcga
    15361 caaggccttc ggcgccaaca aggtgctggc cgagctcgac ctcgacgtcg aacccggcga
    15421 gcgggtcgtc atcatcggcc cctccgggtc gggcaagacc acgatcctgc gcgtgatcat
    15481 gaccctcgag cgccccgacg ccggcacgat ccaggtcggt gggcggcacc tctaccacga
    15541 ggtccacggc aacgggctca agccggccag cgagaagcac atccgctcgg t
//
LOCUS       DATISU010000467         2667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_558740, whole genome shotgun sequence.
ACCESSION   DATISU010000467 DATISU010000000
VERSION     DATISU010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558740"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /gene="arc"
                     /locus_tag="VLR27_18540"
     CDS             <1..1182
                     /gene="arc"
                     /locus_tag="VLR27_18540"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HSP05510.1"
                     /translation="DGLFRLSGWDWQLLALWPTEVEELVLEEVPDVGYDDIGGLDDQI
                     EQIADAVELPFLHQELFAEFELPAPKGILLYGPPGCGKTLIAKAVANSLAKKVAESTG
                     NEARSFFLNIKGPELLNKYVGETERQIRLVFQRAREKSEEGWPVIVFFDEMDSMFRTR
                     GSGISSDMESTIVPQLLAEIDGVESLKNVIVIGASNREDLIDPAILRPGRLDVKIKID
                     RPNEISAAQIFARYLTDSLPIDGGEVQSLGGGDVHKAIAAMVESTVEEMYSTEDRNRF
                     LEVTYQNGDKEVMFFKDFSSGAMIENIVRRAKKLAIKRLLAGGERGIRTEDLLASIKQ
                     EFKEHEDLPNTTNPDDWAKISGKKGERIVYVRTLIQKDPEDEDLQGGRSIERVATGQY
                     L"
     gene            1201..>2667
                     /gene="dop"
                     /locus_tag="VLR27_18545"
     CDS             1201..>2667
                     /gene="dop"
                     /locus_tag="VLR27_18545"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HSP05511.1"
                     /translation="MAVPKIVGIETEFGIVTRGGGESNPITASSVLINAYVQELVRTG
                     QSARTAPRVGWDFVDKMPHVDARGTTPGDARPPEVETHLVNAVLTNGARYYVDHAHPE
                     CSTPECADAREIVVWDRAAEEILRRSMAAAGSLLGPGEEILVHKNNSDGKGNSYGCHE
                     NYLLARETPFGRIVRQITPFFVTRQVFAGAGKVGVEAPGLTSQDVPYQLSQRADFFEE
                     EVGLETTLKRPIVNTRDEPHADAQKYRRLHVIIGDANMSEVATYLKVGTTAIVLAMIE
                     DDELGLDLMPATPIAALRQVSYDVGLSRPFELAAGGTITALEAQWEYFDRARKYAETR
                     GLDAVGADVGADVMARWEAVLTALETDPVQAADVVDWVAKRRLLEGYRDRHDLEWGDP
                     RLAAIGLQYHDLRPERCLAERAGLQRLVEPAEVAHAVSNPPESTRAYFRGRCLQKWPD
                     AIVAANWDSLVFDVGGDPLRRVPMMEPSRGTAEHVGTLI"
BASE COUNT          460 a          940 c          896 g          371 t
ORIGIN      
        1 gacgggctct ttcgtttaag cggctgggat tggcaactcc tcgcgttgtg gccgaccgag
       61 gtcgaggagc tcgtgctcga ggaggtgccc gacgtcggct acgacgacat cggcggcctc
      121 gacgaccaga tcgagcagat cgccgacgcg gtggagctgc cgttcctgca ccaggagctc
      181 ttcgccgagt tcgagctccc ggcgccgaag gggatcctcc tgtacggccc gcccgggtgt
      241 ggcaagaccc tcatcgccaa ggcggtggcc aacagcctgg ccaagaaggt ggcggagagc
      301 accggcaacg aggcccgcag cttcttcctc aacatcaagg gccccgagct gctgaacaag
      361 tacgtcggcg agaccgagcg ccagatccgc ctcgtgttcc agcgggcccg ggagaagagc
      421 gaggagggtt ggccggtcat cgtgttcttc gacgagatgg actcgatgtt ccgcacccgc
      481 gggtcgggca tcagctccga catggagtcc accatcgtcc cccagctgct cgccgagatc
      541 gacggcgtcg agtcgctgaa gaacgtcatc gtcatcggtg cctcgaaccg cgaggacctg
      601 atcgacccgg ccatcctgcg cccggggcgc ctcgacgtga agatcaagat cgaccgcccc
      661 aacgagatct ccgcggccca gatcttcgcc cgctacctca ccgactcgct gcccatcgac
      721 ggcggcgagg tccagtccct cggcggcggc gacgtgcaca aggccatcgc ggcgatggtc
      781 gagtccacgg tcgaggagat gtactccacc gaggaccgca accggttcct cgaggtcacg
      841 taccagaacg gtgacaagga ggtgatgttc ttcaaggact tcagctccgg ggccatgatc
      901 gagaacatcg tgcggcgggc caagaagctg gccatcaagc ggctcctggc cggcggcgag
      961 cgcggcatcc gcaccgagga cctcctcgcc tccatcaagc aggagttcaa ggagcacgag
     1021 gacctgccca acaccaccaa ccccgacgac tgggccaaga tctcgggcaa gaagggcgag
     1081 cggatcgtct acgtgcgcac gctcatccag aaggaccccg aggacgagga cctgcagggc
     1141 ggccgttcca tcgagcgggt cgccaccggt cagtacctgt agggaccgtc gacgaccgcc
     1201 atggctgtcc ccaagatcgt cggcatcgag accgagttcg gcatcgtcac gcgaggtggc
     1261 ggggagtcga acccgatcac cgcgtcctcg gtgctcatca acgcctacgt gcaggagctg
     1321 gtgcggaccg gccagtcggc ccgcaccgcc ccccgggtgg gttgggactt cgtggacaag
     1381 atgccccacg tggacgcccg gggcaccacg cccggcgatg cccggccacc ggaggtcgag
     1441 acccacctgg tcaacgccgt gctcaccaac ggggctcgct actacgtcga ccacgcccac
     1501 ccggagtgct ccaccccgga gtgcgccgat gcccgcgaga tcgtcgtgtg ggaccgggcg
     1561 gcggaggaga tcctgcggcg ctcgatggcc gccgccggca gcctgctcgg gcccggtgaa
     1621 gagatcctcg tccacaagaa caactccgac ggcaagggca acagctacgg ctgccacgag
     1681 aactacctgc tcgcccgcga gacccccttc ggccgcatcg tccgccagat cacgcccttc
     1741 ttcgtcaccc gccaggtgtt cgccggcgcc ggcaaggtcg gtgtcgaggc gccgggcctc
     1801 acgtcccagg acgtgcccta ccagctgtcc cagcgggccg acttcttcga ggaggaggtc
     1861 ggcctcgaga ccacgctgaa gcggcccatc gtcaacaccc gcgacgaacc ccacgccgac
     1921 gcccagaagt accgccgcct gcacgtcatc atcggcgacg ccaacatgag cgaggtcgcc
     1981 acctacctga aggtgggcac caccgcgatc gtgctggcga tgatcgagga cgacgagctc
     2041 gggctcgacc tcatgccggc cacacccatc gccgccctgc gccaggtgtc ctacgacgtg
     2101 gggctgtcga ggccgttcga gctggccgcg ggcggcacca tcaccgcgct cgaggcccag
     2161 tgggagtact tcgaccgggc ccgcaagtac gccgagaccc gggggctgga cgctgtcggc
     2221 gcggacgtcg gcgccgacgt catggcccgg tgggaagccg tgctcaccgc cctcgagacc
     2281 gatccggtgc aggcggctga cgtcgtcgac tgggtggcca agcgccggct gctcgagggc
     2341 taccgggacc gccacgacct cgagtggggc gacccgcggc tggcggcgat cgggctgcag
     2401 taccacgacc tccgacccga gcggtgcctg gcggagcgag ccggcctgca gcgcctggtc
     2461 gagcccgccg aggtcgccca cgccgtgtcg aacccgccgg agtccacccg cgcctacttc
     2521 cggggacggt gcctgcagaa gtggcccgac gccatcgtgg ccgcgaactg ggactcgctg
     2581 gtgttcgacg tgggcggcga cccgctgcgg cgggtgccga tgatggagcc gtcgcgtgga
     2641 accgccgagc acgtgggtac attgatc
//
LOCUS       DATISU010000468         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_605432, whole genome shotgun sequence.
ACCESSION   DATISU010000468 DATISU010000000
VERSION     DATISU010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605432"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1562)
                     /locus_tag="VLR27_18550"
     CDS             complement(<1..1562)
                     /locus_tag="VLR27_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSP05512.1"
                     /translation="MHDLVIRGGTVVDGSGAPARAADIAIDGDRIVEVVDRDDGGSAG
                     PGRREIDATDLLVTPGWVDVHTHYDAQATWDPYLTPSGFHGVTTVVMGNCGVGFAPVA
                     PDRRDWLIGLMEGVEDIPGAAMSDGIEWEWEDFEGYLDALDRRRYVCDVGAQIPHGAL
                     RTYVMGERGAANEPATADDIARMAAMVESGLRAGALGFSTSRTPIHKSVDGELVPGTT
                     AGTDEILGIGEAIRRVGHGVFEYSPDHVKVGDEFGWMRDLAALTGQPVSFNLNQPDTD
                     PDYWQTSLRLLEEAAADGLPVVAQVAGRAIGILFAFELTLHPFLFKPAWGEMAFLEPA
                     EKAARFQDPAWRQRVLDEEPIDLGGDFARFVTTTWERMFPFEGDSSYEPGYEDSVAGI
                     AAATGRRPEEVAYDHLARDGGTGMLYFPLFNYAYGDLELTRQLQLHPQTRMGLSDGGA
                     HCGAVCDGGMPTFMLSYWARDRTQGSGPMPLEHVVHRQTRQTAELYGLLDRGLLAPGM
                     RADVNVIDHATLG"
     gene            complement(1589..>2146)
                     /locus_tag="VLR27_18555"
     CDS             complement(1589..>2146)
                     /locus_tag="VLR27_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05513.1"
                     /translation="HGRFGVCPVTDSAGDALSLALQGAMADLESAVSAPVPDDCLLVV
                     DGPLRERRGMDHAVGYVKTHHVAYLPAVVQDVIPMLEPGQRTPVLLIGDRFRRWSWYL
                     RLPGDRSHGWAGVVRCEISPDRTVADAASVADACTLALPRFASEAHKDSRAPQNLYPI
                     AGLERELRHRLGDARLLYRSLRQAA"
BASE COUNT          290 a          715 c          855 g          286 t
ORIGIN      
        1 ccgagggtgg cgtggtcgat cacgttgacg tcggcccgca tccccggcgc cagcaggccg
       61 cggtcgagca gcccgtagag ctcggcggtc tggcgcgtct ggcggtggac gacgtgctcg
      121 aggggcatcg ggcccgaccc ctgcgtgcgg tcccgcgccc agtagctgag catgaaggtg
      181 ggcatgccgc cgtcgcacac ggcgccgcag tgggcaccgc cgtcggacag gcccatgcgg
      241 gtctgggggt gcagctgcag ctggcgggtg agctcgaggt cgccgtaggc gtagttgaag
      301 agcgggaagt acagcatccc ggtgccgccg tcgcgggcga ggtggtcgta ggcgacctcc
      361 tcgggtcgcc ggccggtggc cgcggcgatg ccggcgacgg agtcctcgta gcccggctcg
      421 taggacgagt cgccctcgaa cgggaacatg cgctcccacg tggtggtgac gaagcgggcg
      481 aagtcaccgc cgaggtcgat gggctcctcg tcgaggacgc gctgccgcca cgccgggtcc
      541 tggaaccggg ccgccttctc ggccggctcg aggaacgcca tctcgcccca cgccggcttg
      601 aacaggaacg ggtgcagggt gagctcgaac gcgaagagga tgccgatggc gcgccccgcc
      661 acctgggcga cgacgggcag cccgtcggcg gcggcctcct cgaggaggcg cagcgacgtc
      721 tgccagtagt cggggtcggt gtcgggctgg ttgaggttga agctgaccgg ctggccggtg
      781 agggcggcca ggtcgcgcat ccagccgaac tcgtcgccga ccttgacgtg gtcggggctg
      841 tactcgaaca ctccgtggcc gacgcggcgg atggcctcgc cgatgccgag gatctcatcg
      901 gtgccggcgg tggtgccggg cacgagctcg ccgtcgacgg acttgtggat cggggtgcgg
      961 ctggtggaga agcccagggc accggcccgc aggccggact cgaccatggc ggccatgcgg
     1021 gcgatgtcgt cggcggtggc cggctcgttc gccgcacccc gctcgcccat cacgtacgtg
     1081 cgcagggccc cgtgggggat ctgcgcgccc acgtcgcaga cgtagcggcg gcggtcgagg
     1141 gcgtcgaggt agccctcgaa gtcctcccac tcccactcga tgccgtcgga catggcggcg
     1201 ccggggatgt cctcgacgcc ctccatcagt ccgatgagcc agtcccgccg gtcgggggcg
     1261 accggggcga agcccacccc gcagttgccc atgacgacgg tggtgacgcc gtggaagccc
     1321 gacggggtga ggtacgggtc ccatgtggcc tgggcgtcgt agtgggtgtg cacgtcgacc
     1381 cagccgggcg tgaccagcag gtcggtggca tcgatctccc ggcgtccggg acccgcggac
     1441 ccaccatcgt cgcggtcgac cacctcgacg atgcggtcgc cgtcgatggc gatgtcggcg
     1501 gcccgggccg gtgcgccgga gccgtcgacg accgtccccc ctcggatgac caggtcgtgc
     1561 atggcccgat ggtagggcgg gctcgggcct acgcggcttg acggagcgac cggtacagga
     1621 gtcgggcgtc gccgaggcgg tggcgcagct cccgctcgag gcccgcgatg gggtacaggt
     1681 tctgcggcgc ccgggagtcc ttgtgggcct cggaggcgaa ccggggcagg gcgagcgtgc
     1741 aggcatcggc caccgacgcg gcatcggcca cggtgcggtc ggggctgatc tcgcaacgca
     1801 cgaccccggc ccagccgtgg gaccggtcgc cgggcaggcg caggtaccag gaccagcgcc
     1861 ggaagcggtc gccgatgagc agcaccggcg tgcgctggcc aggctcgagc atcgggatga
     1921 cgtcctggac gaccgcgggc aggtaggcga cgtggtgggt cttgacgtag ccgacggcgt
     1981 ggtccatgcc ccgccgctcg cgcaacgggc cgtcgacgac cagcaggcag tcgtcgggga
     2041 ccggcgccga gacggcggac tcgaggtcgg ccatggcgcc ctggagcgcg agggagaggg
     2101 cgtcgccggc gctgtcggtg accgggcaga cgccgaagcg gccgtg
//
LOCUS       DATISU010000469         1701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_791579, whole genome shotgun sequence.
ACCESSION   DATISU010000469 DATISU010000000
VERSION     DATISU010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791579"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1047)
                     /gene="rsmI"
                     /locus_tag="VLR27_18560"
     CDS             complement(226..1047)
                     /gene="rsmI"
                     /locus_tag="VLR27_18560"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HSP05514.1"
                     /translation="MTGRLVVVGTPIGNLGDLSPRAVQSLTDADVIACEDTRRTGRLL
                     AAAGIDRTDLVVANDHTEARVAGRLVERARAGAVVALVTDAGMPGVSDPGNDVVAAFV
                     DAGLGVEVVPGPTAVTTALAASGLPTGRFVFEGFLPRKGAGRTERLAAVAAEPRTVVL
                     YEAPHRAERTIADLAGACGADRPVTVARELTKLHEEWFRGDLAGAAEWLAAEPKGEFV
                     IVLGGAAPPPEVTDDELREALAEEVAAGATTRDAVAAVVARYGVAKRRVYDLAIR"
     gene            complement(1044..1391)
                     /locus_tag="VLR27_18565"
     CDS             complement(1044..1391)
                     /locus_tag="VLR27_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HSP05515.1"
                     /translation="MPWLLRDDRVLASLEIASSRSDRRRGLLGRDGFEGALLIEGTRS
                     VHTIGMRFPIDVAHLDDEGTVLRCGTMRRHRLGMPVLRARKVLEAEAGSFERWGICPG
                     QVLEVRVQHPDTP"
     gene            complement(1448..>1701)
                     /locus_tag="VLR27_18570"
     CDS             complement(1448..>1701)
                     /locus_tag="VLR27_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HSP05516.1"
                     /translation="TYFTTGEKESRAWTFRAGSKAPEAAGVIHTDFQRGFIRAECIQW
                     DELLECGSWNAAKDVGKLRVEGKDYVVQDGDVLEFRFNV"
BASE COUNT          236 a          625 c          624 g          216 t
ORIGIN      
        1 gaccaccacg tcttcgtgct ccacaccgac gaggaggagg cggtctggga gctcagcgcc
       61 gacggcgtcg ccggcctcca ccaggcgctc ctcgccaccc accgcccgtc gacgatcgac
      121 gaggccctcg ccgccgccaa cggctgagga acgaacgcgc cgatcggttc gttcgttcca
      181 cccagcggaa ccaacggacc gatcggttcg ttggtccccg gctcctcagc ggatggcgag
      241 gtcgtagacc cggcgcttgg cgacgccgta gcgggcgacc acggcggcga cggcgtcgcg
      301 ggtggtggcg ccggcggcga cctcctcggc gagggcctcg cgcagctcgt cgtcggtgac
      361 ctccggaggc ggggcggccc cgccgagcac gatgacgaac tcgcccttgg gctcggcggc
      421 cagccactcg gcggcgccgg cgagatcgcc ccggaaccac tcctcgtgga gcttggtcag
      481 ctcccgggcc acggtgacgg gacggtcggc gccgcaggcc ccggccaggt ccgcgatggt
      541 tcgctcggcg cggtgcggtg cctcgtacag caccacggtg cggggttcgg cggcgacggc
      601 ggcgaggcgc tcggtccgac cggcaccctt gcggggcagg aagccctcga acacgaaccg
      661 cccggtgggg agcccgctgg ccgccagcgc ggtggtgacc gcggtgggtc cgggcacgac
      721 ctccacgccg agcccggcgt cgacgaacgc ggcgaccacg tcgttgcccg gatcggagac
      781 gccgggcatg ccggcgtcgg tcaccagcgc cacgaccgcc ccggcgcggg cccgctcgac
      841 cagccggccg gcgacccgtg cttcggtgtg gtcgttggcg acgacgaggt cggtccgatc
      901 gatcccggcg gcggccagca accggccggt gcggcgggtg tcctcgcagg cgatgacgtc
      961 ggcatcggtg agggactgca ccgcccgggg cgacaggtca ccgaggttgc cgatgggcgt
     1021 gccgacgacg acgaggcgcc cggtcacggg gtgtcggggt gctggacgcg gacctcgagg
     1081 acctggccgg ggcagatccc ccaccgctcg aacgagccag cctcggcctc cagcaccttc
     1141 cgggcccgca ggacgggcat gccgagtcgg tgtcgacgca tcgtcccgca gcgcaggacc
     1201 gtgccctcgt cgtcgaggtg ggccacgtcg atcgggaacc gcatgccgat cgtgtggacc
     1261 gaccgggtgc cctcgatcag gagcgcaccc tcgaagccgt cacggccgag gagcccccgg
     1321 cgccggtcgg accgggacga ggcgatctcg aggctcgcca gcacccggtc gtcacgcagg
     1381 agccacggca tgggcgcatt ttgcccgacg gatgcgcagc agccgtcaac gaacccgaag
     1441 ggctcgctca gacattgaag cggaactcga ggacgtcgcc gtcctggacc acgtagtcct
     1501 tgccctcgac ccggagcttg cccacgtcct tggccgcgtt ccacgagccg cactcgagca
     1561 gctcgtccca ctggatgcac tcggcccgga tgaagccgcg ctggaagtcg gtgtggatga
     1621 cgccggcggc ctcgggcgcc ttggagccgg cccggaacgt ccacgcccgg ctctccttct
     1681 cgcccgtggt gaagtacgtg c
//
LOCUS       DATISU010000470         4272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U15937
            k141_838381, whole genome shotgun sequence.
ACCESSION   DATISU010000470 DATISU010000000
VERSION     DATISU010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936647
            Sequence Read Archive: SRR9691033
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 09:35:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,719
            CDSs (total)                      :: 3,678
            Genes (coding)                    :: 3,672
            CDSs (with protein)               :: 3,672
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838381"
                     /isolate="SMAG_U15937"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="China"
                     /lat_lon="29.19 N 90.59 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VLR27_18575"
     CDS             <1..417
                     /locus_tag="VLR27_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSP05517.1"
                     /translation="SIRILVAGDEPFGPRAALAASLMPWDVVQDLPVTPSGLWEVCWR
                     QALEEGRIETIHHHGPFVDCADAVDYLRANLASAGGSVVGEGAVVEGTIEESVVWPGA
                     RVREREHLVRAIRTDADTTVLVRRDVSMRLDHDHRH"
     gene            complement(473..1528)
                     /gene="rlmN"
                     /locus_tag="VLR27_18580"
     CDS             complement(473..1528)
                     /gene="rlmN"
                     /locus_tag="VLR27_18580"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HSP05518.1"
                     /translation="MPTRYDLDREAFADLLADEPRYRVDQVWQGLYEHHTDPADITNL
                     PKALRARLADELPLALEPAAESVSDDGDTVKWLWRLADGAAVETVLMHYPDRSTVCVS
                     SQAGCAMACSFCATGQAGFERQMTVGEIVEQVVIAARVAKPRRLSNVVFMGMGEPLAN
                     YDATWGAVERIHGALGLSARHITVSTVGVVPGIERLTQESLPVNLAVSLHAANDELRD
                     ELVPLNRRYPIARLLEACSRYVDTTHRRLSFEWALIDGVNDREQDAQELAVAARAVRA
                     HVNLIPLNPTPGYAVRGTPPQRVREFRDRLEQLGVNATVRRNRGTEIDAACGQLRANH
                     EATPVRAPRRRTRTSTP"
     gene            1593..2336
                     /locus_tag="VLR27_18585"
     CDS             1593..2336
                     /locus_tag="VLR27_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HSP05519.1"
                     /translation="MVDPLTSDPDGVRPEPPVAEHQHRDVRGGAARAAVFGVSDGLVS
                     NVGLILGVAGADPAPAVVRVAGLAGLVAGAISMAAGEYNSMRVQAELFEREVALERIE
                     IRRNPHVETAELDQLYQSRGVAPAAARALAESVMADEELAVRTHAREELGIDPDELGS
                     PTGAAVSSFAAFCAGAVIPLVPWFITSGMAATVASLIAALVAAVIIGVVIARFTEKPA
                     WRVAGRQLAFTAVPAALTYTIGTLVGVNV"
     gene            complement(2415..3314)
                     /gene="mshD"
                     /locus_tag="VLR27_18590"
     CDS             complement(2415..3314)
                     /gene="mshD"
                     /locus_tag="VLR27_18590"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HSP05520.1"
                     /translation="MAELQIKHHLDDVDVREVEHLIERVYLADGHRPIDEHRWVDAAH
                     GGRSEFAGLVLTEEGHDHLVAYSQVTRGEQSWAIDLVIDPHHRYDALIIAPRLLGAAV
                     DLIAEEGGGHVHYWVYEPNEAHTRIANLVGLAPGRDLWQMQVPLPLAETTDLVTRPFV
                     PGQDEEAWLEVNNAAFAWHPEQGGWTLDDVRQREDEPWFDPEGFLLHERDGRLAGFCW
                     TKVHEEPPVGEIYVIAVHPDFAGQGLGRALTVAGLTHLAATTDTGMLYVDADNTPAVK
                     LYEDLGFTVQSTNRAFVGDVKPS"
     gene            3357..4100
                     /gene="nth"
                     /locus_tag="VLR27_18595"
     CDS             3357..4100
                     /gene="nth"
                     /locus_tag="VLR27_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HSP05521.1"
                     /translation="MAARVQNPPPPPGRRRPRTAAGRARATHEALAEAYPGTAVELCE
                     LDHRDPFELLVATILSAQCTDERVNMTTPEVFARWPDPESLAGADPEELEQVIHPTGF
                     FRQKAKSLLGMSARLLEHHDGEVPHRIDDLVALPGVGRKTANVIRSVALDEPGLPVDT
                     HVGRVAIRLGLTEETDPVKVEHELGSMVPASERGLLSLRLILHGRRTCFARRPACAEC
                     PLAWFCPSFGIANLPTSPHKPKKSRSGGI"
BASE COUNT          559 a         1538 c         1554 g          621 t
ORIGIN      
        1 tccatccgca tcctcgtcgc cggcgacgaa ccgttcgggc cccgtgccgc gctggccgcg
       61 tcgttgatgc cgtgggacgt cgtgcaggac ctgcccgtca ccccgtcggg gctgtgggag
      121 gtgtgctggc gccaggcgct cgaggagggt cggatcgaga cgatccacca ccacggcccc
      181 ttcgtcgact gcgccgacgc cgtcgactac ctgcgcgcca acctcgcatc cgccggcggc
      241 agcgtcgtcg gcgagggcgc cgtcgtcgag ggcaccatcg aggagtcggt ggtgtggccg
      301 ggcgcccggg tgcgcgagcg ggagcacctc gtgcgcgcca tccgcaccga cgccgacacc
      361 accgtgctgg tccgccgcga cgtctccatg cgcctcgacc acgaccaccg ccactgatcc
      421 gttctgggct acatccatga ccgtcaggcg gtacggaggt aggccagaac gctcaggggg
      481 tggaggtgcg ggtgcgccgg cggggggctc ggaccggggt ggcttcgtgg ttggcccgga
      541 gctggccgca cgccgcgtcg atctcggtgc cgcggttgcg ccggacggtg gcgttcacgc
      601 cgagctgctc gaggcggtcg cggaactcgc gcacccgctg cggcggcgtg ccccgcaccg
      661 cgtagccggg cgtcgggttg agcgggatga ggttgacgtg ggctcgcaca gcccgggcgg
      721 cgaccgccag ctcctgggcg tcctgctccc ggtcgttcac gccgtcgatg agcgcccact
      781 cgaacgacag gcgccggtgg gtggtgtcga cgtagcggct gcaggcctcg aggagccggg
      841 cgatggggta gcggcggttg agggggacca gctcgtcgcg cagctcgtcg ttcgcggcgt
      901 gcagggacac ggccaggttc acgggcagcg actcctgggt gagccgctcg atgccgggca
      961 ccacgcccac ggtggacacc gtgatgtggc gggcggacag gccgagtgcg ccgtggatgc
     1021 gctcgaccgc accccacgtg gcgtcgtagt tggcgagcgg ctcgcccatg cccatgaaca
     1081 cgacgttcga caggcgccgg ggcttggcca cgcgggcggc gatgacgacc tgctcgacga
     1141 tctcgccgac ggtcatctgg cgctcgaagc cggcctggcc ggtggcgcag aagctgcagg
     1201 ccatggcgca gcccgcctgg ctcgacacgc acaccgtgga gcggtcgggg tagtgcatga
     1261 gcacggtctc gaccgccgca ccgtcggcga ggcgccacag ccacttgacc gtgtctccgt
     1321 cgtcgctgac cgactcggcg gccggctcga gggcgagggg cagctcgtcg gcgaggcggg
     1381 cccgcagcgc cttgggcagg ttggtgatgt cggccggatc ggtgtggtgc tcgtagaggc
     1441 cctgccacac ctggtcgacc cggtagcgcg gctcatcggc gagcaggtcg gcgaaggcct
     1501 cgcgatcgag gtcgtagcgg gtgggcacga tcgaccagcg tacgggccgc gggatgggcg
     1561 gccacccgtg ggcgacgccg tagcctggtc ccgtggtcga ccctctcacc agcgatcccg
     1621 acggggtccg ccccgaaccg ccggtcgccg agcaccagca ccgcgacgtg cggggtgggg
     1681 cggcccgggc tgcggtgttc ggggtgagcg acggcctcgt ctccaacgtc ggcctgatcc
     1741 tcggcgtcgc cggcgccgat ccggccccgg ctgtcgtccg ggtggccggc ctggccgggc
     1801 tcgtcgccgg agccatctcc atggcggccg gtgagtacaa ctcgatgcgg gtccaggccg
     1861 agctgttcga gcgcgaggtc gccctcgagc gcatcgagat ccgccgaaac ccccacgtgg
     1921 agaccgccga gctggatcag ctgtaccagt cccgcggcgt ggcgcctgcc gcggcccgtg
     1981 cgctggccga gtcggtcatg gccgacgagg agctggccgt acgcacccac gcccgcgagg
     2041 agctgggcat cgaccccgac gagctcggct cgcccaccgg tgccgccgtg tcctccttcg
     2101 ctgctttctg tgccggcgcc gtcatcccgc tcgtgccgtg gttcatcacc tcgggcatgg
     2161 ccgccaccgt ggcctccctg atcgccgccc tcgtggccgc cgtgatcatc ggcgtcgtca
     2221 tcgcccgctt caccgagaag ccggcctggc gggtcgccgg tcgccagctc gccttcaccg
     2281 cggtgcccgc cgccctcacc tacacgatcg gcaccctcgt cggcgtcaac gtctgaccga
     2341 aaactggcag ccgaatcgtc acacacgggc ttcgtgtgac gaggcggctg ccagtttcca
     2401 gcggatggac cgcctcagga cggcttcaca tctcccacga acgccctgtt ggtggactgc
     2461 acggtgaagc cgaggtcctc gtacagcttc acggccgggg tgttgtcggc gtcgacgtag
     2521 agcatgccgg tgtcggtcgt ggcggcgagg tgggtgaggc cggcgacggt gagcgcccga
     2581 cccaaaccct ggccggcgaa gtcggggtgg acggcgatca cgtagatctc gcccaccggg
     2641 ggctcctcgt ggaccttggt ccagcagaac ccggccaggc gcccgtcgcg ctcgtggagc
     2701 aggaagccct cggggtcgaa ccacggctcg tcctcgcgct ggcggacgtc gtcgagggtc
     2761 cagccgccct gctcgggatg ccaggcgaag gccgcgttgt tcacctcgag ccacgcctcc
     2821 tcgtcctggc cgggcacgaa cggccgggtg acgaggtcgg tggtctcggc gagggggagg
     2881 ggcacctgca tctgccacag gtcccggccg ggggcgaggc cgacgaggtt ggcgatgcgg
     2941 gtgtgggcct cgttgggctc gtacacccag tagtggacgt ggcccccgcc ctcctcggcg
     3001 atgaggtcca cggctgcacc gagcaggcgg ggggcgatga tgagggcgtc gtagcggtgg
     3061 tgcgggtcga tgacgaggtc gatggcccac gactgctcgc cccgggtgac ctgggagtag
     3121 gcgaccaggt ggtcgtggcc ctcctcggtg agcaccaggc cggcgaactc ggaccggccg
     3181 ccatgggccg cgtccaccca gcggtgctcg tcgatgggac ggtggccgtc ggcgaggtac
     3241 acccgctcga tcaggtgctc cacctcgcgg acgtcgacgt cgtcgaggtg gtgcttgatc
     3301 tggagctcgg ccatgggcgg ccgaggctac cgaggcccgt cggtagcctc cgacccatgg
     3361 ccgcccgagt gcagaaccct ccgccaccac cgggtcggcg ccgaccccgc acggcggcgg
     3421 gccgggcccg cgccacccac gaggcgctcg ccgaggccta cccgggcacc gccgtggagc
     3481 tctgcgagct cgaccaccgc gaccccttcg agctgctcgt cgccacgatc ctgtcggccc
     3541 agtgcaccga cgagcgcgtg aacatgacca cccccgaggt gttcgcccgc tggcccgacc
     3601 ccgagtccct cgccggcgcc gacccggagg agctggagca ggtcatccac cccaccgggt
     3661 tcttccgcca gaaggccaag tcgctgctcg ggatgtcggc ccgcctgctc gagcaccacg
     3721 acggcgaggt cccgcaccgc atcgacgacc tcgtcgccct ccccggcgtg gggcgcaaga
     3781 cggccaacgt catccgctcg gtggccctcg acgagcccgg cctgccggtc gacacccacg
     3841 tcgggcgggt cgccatccgc ctcggcctca ccgaggagac cgacccggtg aaggtggagc
     3901 acgagctggg gtcgatggtg ccggcgagcg agcgtggact gctcagcctg cggctgatcc
     3961 tgcacggccg ccgcacgtgc ttcgcccgcc gcccagcgtg cgcggagtgc ccgctggcat
     4021 ggttctgccc gtcgttcggg atcgcgaatc tgcccacttc accccacaaa ccgaaaaaat
     4081 ctcggagtgg tggcatctag tccgtccgga tcatgtagcg tttctctcag tccaggttcc
     4141 cgccacctgg aactgacgcg ttgcacggga tccgccgccc ggcaacgcca gcgacggcgc
     4201 ccactcccac ccgggcgccg tcgccgcgtc acggggtcgg acaccacggt gttcggcccc
     4261 tgacccgcgc cc
//
